LOCUS       DAZFLD010000001        74355 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.001, whole genome shotgun sequence.
ACCESSION   DAZFLD010000001 DAZFLD010000000
VERSION     DAZFLD010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 74355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 74355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..74355
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.001"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            117..641
                     /gene="aroL"
                     /locus_tag="ACHKBL_000001"
     CDS             117..641
                     /gene="aroL"
                     /locus_tag="ACHKBL_000001"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroL"
                     /protein_id="HFO5816933.1"
                     /translation="MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMT
                     VAEIVEREEWAGFRARETAALEAVTAPSTVIATGGGIILTEFNRHFMQNNGIVVYLCA
                     PVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPSQV
                     ISEIRSALAQTINC"
     gene            691..882
                     /gene="yaiA"
                     /locus_tag="ACHKBL_000002"
     CDS             691..882
                     /gene="yaiA"
                     /locus_tag="ACHKBL_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YaiA"
                     /protein_id="HFO5816934.1"
                     /translation="MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRADHPKPDSLISEH
                     PTAQEAMDAKKRYEDPDKE"
     gene            1140..1817
                     /gene="aroM"
                     /locus_tag="ACHKBL_000003"
     CDS             1140..1817
                     /gene="aroM"
                     /locus_tag="ACHKBL_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HFO5816935.1"
                     /translation="MSASLAILTIGIVPMQEVLPLLTEYIDEDNISHHSLLGKLSREE
                     VMAEYAPEAGEDTILTLLNDNHLAHVSRRKVERDLQGVVEVLDNQGYDVIILMSTANI
                     SSMTARNTIFLEPSRILPPLVSSIVEDHQVGVIVPVEELLTVQAQKWQILQKPPVFSL
                     GNPIHDSEQKIIDAGKELLAKGADVIMLDCLGFNQRHRDLLQKQLDVPVLLSNVLIAR
                     LAAELLM"
     gene            1889..2173
                     /gene="ppnP"
                     /locus_tag="ACHKBL_000004"
     CDS             1889..2173
                     /gene="ppnP"
                     /locus_tag="ACHKBL_000004"
                     /EC_number="2.4.2.1"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HFO5816936.1"
                     /translation="MLQSNEYFSGKVKSIGFSSSSTGRASVGVMVEGEYTFSTAEPEE
                     MTVISGALNVLLPDATDWQVYEAGSLFNVPGHSEFHLQVAEPTSYLCRYL"
     gene            2330..2659
                     /locus_tag="ACHKBL_000005"
                     /pseudo
     CDS             2330..2659
                     /locus_tag="ACHKBL_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308469.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
     gene            2656..2736
                     /locus_tag="ACHKBL_000006"
     CDS             2656..2736
                     /locus_tag="ACHKBL_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5816937.1"
                     /translation="MTQRPWSKLQHKTHNIAALRIIARCS"
     gene            complement(2817..3728)
                     /gene="rdgC"
                     /locus_tag="ACHKBL_000007"
     CDS             complement(2817..3728)
                     /gene="rdgC"
                     /locus_tag="ACHKBL_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HFO5816938.1"
                     /translation="MLWFKNLMVYRLSREISLRAEEMEKQLASMAFTPCGSQDMAKMG
                     WVPPMGSHSDALTHVANGQIVICARKEEKILPSPVIKQALEAKIAKLEAEQARKLKKT
                     EKDSLKDEVLHSLLPRAFSRFSQTMMWIDTVNGLIMVDCASAKKAEDTLALLRKSLGS
                     LPVVPLSMENPIELTLTEWVRSGSAAQGFQLLDEAELKSLLEDGGVIRAKKQDLTSEE
                     ITNHIEAGKVVTKLALDWQQRIQFVMCDDGSLKRLKFCDELRDQNEDIDREDFAQRFD
                     ADFILMTGELAALIQNLIEGLGGEAQR"
     gene            3853..4761
                     /gene="mak"
                     /locus_tag="ACHKBL_000008"
     CDS             3853..4761
                     /gene="mak"
                     /locus_tag="ACHKBL_000008"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
                     /protein_id="HFO5816939.1"
                     /translation="MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIA
                     TLVDMAEQATGQRGTVGMGIPGSISPYTGVVKNANSTWLNGQPFDKDLSARLQREVRL
                     ANDANCLAVSEAVDGAAAGAQTVFAVIIGTGCGAGVAFNGRAHIGGNGTAGEWGHNPL
                     PWMDEDELRYREEVPCYCGKQGCIETFISGTGFATDYRRLSGHALKGSEIIRLVEESD
                     PVAELALRRYELRLAKSLAHVVNILDPDVIVLGGGMSNVDRLYQTVPQLIKQFVFGGE
                     CETPVRKAKHGDSSGVRGAAWLWPQE"
     gene            complement(4900..6084)
                     /gene="araJ"
                     /locus_tag="ACHKBL_000009"
     CDS             complement(4900..6084)
                     /gene="araJ"
                     /locus_tag="ACHKBL_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414930.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter AraJ"
                     /protein_id="HFO5816940.1"
                     /translation="MKKVILSLALGTFGLGMAEFGIMGVLTELAHNVGISIPAAGHMI
                     SYYALGVVVGAPIIALFSSRYSLKHILLFLVALCVIGNAMFTLSSSYLMLAIGRLVSG
                     FPHGAFFGVGAIVLSKIIKPGKVTAAVAGMVSGMTVANLLGIPLGTYLSQEFSWRYTF
                     LLIAVFNIAVMASVYFWVPDIRDEAKGKLREQFHFLRSPAPWLIFAATMFGNAGVFAW
                     FSYVKPYMMFISGFSETAMTFIMMLVGLGMVLGNVLSGRISGRYSPLRIAAVTDFIIV
                     LALLMLFFFGGMKTTSLIFAFICCAGLFALSAPLQILLLQNAKGGELLGAAGGQIAFN
                     LGSAVGAYCGGMMLTLGLAYNYVALPAALLSFAAMSSLLLYGRYKRQQAADSPVLAKP
                     LG"
     gene            complement(6210..9353)
                     /gene="sbcC"
                     /locus_tag="ACHKBL_000010"
     CDS             complement(6210..9353)
                     /gene="sbcC"
                     /locus_tag="ACHKBL_000010"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcC"
                     /protein_id="HFO5816941.1"
                     /translation="MKILSLRLKNLNSLKGEWKIDFTREPFASNGLFAITGPTGAGKT
                     TLLDAICLALYHETPRLSNVSQSQNDLMTRDTAECLAEVEFEVKGEAYRAFWSQNRAR
                     NQPDGNLQVPRVELARCADGKILADKVKDKLELTATLTGLDYGRFTRSMLLSQGQFAA
                     FLNAKPKERAELLEELTGTEIYGQISSMVFEQHKSARTELEKLQAQASGVALLTPEQV
                     QSLTASLQVLTDEEKQLLTAQQQEQQSLNWLTRLDELQQEASRRQQALQQALAEEEKA
                     QPQLAALSLAQPARNLRPHWERIAEHSAALAHTRQQIKEVNTRLQSTMALRASIRHHA
                     AKQSAELQQQQQSLNAWLQEHDRFRQWNNELAGWRAQFSQQTSVREHLRQWQQQLTHA
                     EQKLNALTAITLTLTADEVATALAQHAEQRTLRQRLVALHGQIVPQQKRLAQLQVAIQ
                     NVTQEQTQRNAALNEMRQRYKEKTQQLADVKTICEQEARIKTLEAQRAQLQAGQPCPL
                     CGSTSHPAVEAYQALEPGVNQSRLLALENEVKKLGEEGATLRGQLDALTKQLQRDENE
                     AQSLRQDEQALTQQWQAVTASLNITLQPQDDIQPWLDAQDEHERQLRLLSQRHELQGQ
                     IAAHNQQIIQYQQQIEQRQQQLLTALAGYALTLPQEDEEESWLATRQQEAQSWQHRQN
                     ELTALQNRMQQLTPILETLPQSDELPHSEETVVLENWRQVHEQCLALHSQQQTLQQQD
                     VLAAQSLQKAQAQFDTALQASVFDDQQAFLAALMDEQTLTQLEQLKQNLENQRRQAQT
                     LVTQTAETLAQHQQHRPDGLALTVTMEQIQQELAQTHQKLRENTTSQGEIRQQLKQDA
                     DNRQQQQTLMQQIAQMTQQVEDWGYLNSLIGSKEGDKFRKFAQGLTLDNLVHLANQQL
                     TRLHGRYLLQRKASEALEVEVVDTWQADAVRDTRTLSGGESFLVSLALALALSDLVSH
                     KTRIDSLFLDEGFGTLDSETLDTALDALDALNASGKTIGVISHVEAMKERIPVQIKVK
                     KINGLGYSKLESAFAVK"
     gene            complement(9350..10552)
                     /gene="sbcD"
                     /locus_tag="ACHKBL_000011"
     CDS             complement(9350..10552)
                     /gene="sbcD"
                     /locus_tag="ACHKBL_000011"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HFO5816942.1"
                     /translation="MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETAQAHQVDAII
                     VAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNT
                     TVVASAGHAPQILPRRDGTPGAVLCPIPFLRPRDIITSQAGLNGIEKQQHLLAAITDY
                     YQQHYADACKLRGDQPLPIIATGHLTTVGASKSDAVRDIYIGTLDAFPAQNFPPADYI
                     ALGHIHRAQIIGGMEHVRYCGSPIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVT
                     QPMAVLKGDLASITEQLEQWRDVSQEPPVWLDIEITTDEYLHDIQRKIQALTESLPVE
                     VLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEELEESQQQRLQHLFATTLH
                     SLAGEHEA"
     gene            10742..11431
                     /gene="phoB"
                     /locus_tag="ACHKBL_000012"
     CDS             10742..11431
                     /gene="phoB"
                     /locus_tag="ACHKBL_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate response regulator transcription
                     factor PhoB"
                     /protein_id="HFO5816943.1"
                     /translation="MARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNE
                     PWPDLILLDWMLPGGSGIQFIKHLKRESMTRDIPVVMLTARGEEEDRVRGLETGADDY
                     ITKPFSPKELVARIKAVMRRISPMAVEEVIEMQGLSLDPTSHRVMAGEEPLEMGPTEF
                     KLLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEPGGHDRMVQTV
                     RGTGYRFSTRF"
     gene            11489..12784
                     /gene="phoR"
                     /locus_tag="ACHKBL_000013"
     CDS             11489..12784
                     /gene="phoR"
                     /locus_tag="ACHKBL_000013"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HFO5816944.1"
                     /translation="MLERLSWKRLVLELLLCCLPAFILGAFFGYLPWFLLASVTGLLI
                     WHFWNLLRLSWWLWVDRSMTPPPGRGSWEPLLYGLHQMQLRNKKRRRELGNLIKRFRS
                     GAESLPDAVVLTTEEGGIFWCNGLAQQILGLRWPEDNGQNILNLLRYPEFTQYLKTRD
                     FSRPLNLVLNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGARRNFFANVSHELRTP
                     LTVLQGYLEMMDEQPLEGAVREKALHTMREQTQRMEGLVKQLLTLSKIEAAPTHLLNE
                     KVDVPMMLRVVEREAQTLSQKKQTFTFEIDNGLKVSGNEDQLRSAISNLVYNAVNHTP
                     EGTHITVRWQRVPHGAEFSVEDNGPGIAPEHIPRLTERFYRVDKARSRQTGGSGLGLA
                     IVKHAVNHHESRLNIESTVGKVTRFSFVIPERLIAKNSD"
     gene            13191..14510
                     /gene="brnQ"
                     /locus_tag="ACHKBL_000014"
     CDS             13191..14510
                     /gene="brnQ"
                     /locus_tag="ACHKBL_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter carrier
                     protein BrnQ"
                     /protein_id="HFO5816945.1"
                     /translation="MTHQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTA
                     AFGFLITAVGLPVLTVVALAKVGGGVDSLSTPIGKVAGVLLATVCYLAVGPLFATPRT
                     ATVSFEVGIAPLTGDSALPLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALV
                     ILSVAAIIWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGV
                     TEARLLTRYTVWAGLMAGVGLTLLYLALFRLGSDSASLVDQSANGAAILHAYVQHTFG
                     GGGSFLLAALIFIACLVTAVGLTCACAEFFAQYVPLSYRTLVFILGGFSMVVSNLGLS
                     QLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPPMFISLLFGILDGIKASAF
                     SDILPSWAQRLPLAEQGLAWLMPTVVMVVLAIIWDRAAGRQVTSSAH"
     gene            14586..15959
                     /gene="proY"
                     /locus_tag="ACHKBL_000015"
     CDS             14586..15959
                     /gene="proY"
                     /locus_tag="ACHKBL_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline-specific permease ProY"
                     /protein_id="HFO5816946.1"
                     /translation="MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSV
                     LLAYIIGGVAAYIIMRALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIV
                     AIADVTAFGIYMGVWFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
                     IIMIVAGFGIIIWGIGNGGQPTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEII
                     GITAGEAKDPEKSIPRAINSVPMRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQH
                     MGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPKIFSKTSRRGIPWVT
                     VLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRRLPPEEVK
                     SLKFKVPGGVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVVLLIGWMFKRRHD
                     RQLAENH"
     gene            16115..17932
                     /gene="malZ"
                     /locus_tag="ACHKBL_000016"
     CDS             16115..17932
                     /gene="malZ"
                     /locus_tag="ACHKBL_000016"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308480.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004558 - alpha-1,4-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin glucosidase"
                     /protein_id="HFO5816947.1"
                     /translation="MMLNAWHLPVPPFVKQSKDQLLITLWLTGEDPPQRIMLRTEHDN
                     EETSVPMHKQRSQPQPGVTAWRAAIDLSGGLPRRRYSFKLLWHDRQRWFTPQGFSRMP
                     PARLEQFAVDVPDIGPQWAADQIFYQIFPDRFARSLPREAEQDHVYYHHAAGQEIILR
                     DWDEPVTAQAGGSTFYGGDLDGISEKLPYLKKLGVTALYLNPVFKAPSVHKYDTEDYR
                     HVDPQFGGDGALLRLRHNTQQLGMRLVLDGVFNHSGDSHAWFDRHNRGTGGACHNPES
                     PWRDWYSFSDDGTALDWLGYASLPKLDYQSESLVNEIYRGEDSIVRHWLKAPWNMDGW
                     RLDVVHMLGEAGGARNNLQHVAGITEAAKETQPEAYIVGEHFGDARQWLQADVEDAAM
                     NYRGFTFPLWGFLANTDISYDPQQIDAQTCMAWMDNYRAGLSHQQQLRMFNQLDSHDT
                     ARFKTLLGRDIARLPLAVVWLFTWPGVPCIYYGDEVGLDGKNDPFCRKPFPWQVEKQD
                     TALFALYQRMIALRKKSQALRRGGCQVLYAEDNVVVFVRVLYQQRVLVAINRGEACEV
                     VLPASPLLNVAQWQRKEGHGQLTDGILALPAISATVWMN"
     gene            complement(17937..18518)
                     /gene="acpH"
                     /locus_tag="ACHKBL_000017"
     CDS             complement(17937..18518)
                     /gene="acpH"
                     /locus_tag="ACHKBL_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008770 - [acyl-carrier-protein]
                     phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
                     /protein_id="HFO5816948.1"
                     /translation="MNFLAHLHLAHLAESSLSGNLLADFVRGNPEESFPPDVVAGIHM
                     HRRIDVLTDNLPEVREAREWFRNETRRVAPITLDVMWDHFLSRHWSQLSPDFPLQEFT
                     CYAREQVMTILPDSPPRFINLNNYLWSEQWLVRYRDMDFIQSVLNGMASRRPRLDALR
                     NSWYDLDAHYDALETCFWQFYPRMMEQASRKAL"
     gene            18611..19681
                     /gene="queA"
                     /locus_tag="ACHKBL_000018"
     CDS             18611..19681
                     /gene="queA"
                     /locus_tag="ACHKBL_000018"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051075 - S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HFO5816949.1"
                     /translation="MRVTDFSFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFT
                     DLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPK
                     PGAELLLGDDERINATMTARHGALFEVEFNDQRSVLDILNSIGHMPLPPYIDRPDEDA
                     DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVD
                     TIEDHIMHSEYAEVPQDVVDAVLAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPF
                     FDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHTMNAYKAAVEEKYRF
                     FSYGDAMFITYNPQAINERVGE"
     gene            19736..20863
                     /gene="tgt"
                     /locus_tag="ACHKBL_000019"
     CDS             19736..20863
                     /gene="tgt"
                     /locus_tag="ACHKBL_000019"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HFO5816950.1"
                     /translation="MKFELDTTDGRARRGRLVFDRGVVETPCFMPVGTYGTVKGMTPE
                     EVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIR
                     KITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
                     MSLRWAKRSRERFDSLGNKNALFGIIQGSVYEDLRDISVKGLVDIGFDGYAVGGLAVG
                     EPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGH
                     LFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHN
                     LRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD"
     gene            20886..21218
                     /gene="yajC"
                     /locus_tag="ACHKBL_000020"
     CDS             20886..21218
                     /gene="yajC"
                     /locus_tag="ACHKBL_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HFO5816951.1"
                     /translation="MSFFISDAVAATGAPAQGSPMSLILMLVVFGLIFYFMILRPQQK
                     RTKEHKKLMDSIAKGDEVLTNGGLVGRVTKVAENGYIAIALNDTTEVVIKRDFVAAVL
                     PKGTMKAL"
     gene            21246..23093
                     /gene="secD"
                     /locus_tag="ACHKBL_000021"
     CDS             21246..23093
                     /gene="secD"
                     /locus_tag="ACHKBL_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HFO5816952.1"
                     /translation="MLNRYPLWKYVMLIVVIVIGLLYALPNLFGEDPAVQITGARGVA
                     ASEQTLIQVQKTLQEEKITAKSVALEEGAILARFDSTDTQLRAREALMGVMGDKYVVA
                     LNLAPATPRWLAAIHAEPMKLGLDLRGGVHFLMEVDMDTALGKLQEQNIDSLRSDLRE
                     KGIPYTTVRKENNYGLSITFRDAKARDEAIAYLSKRHPDLVISSQGSNQLRAVMSDAR
                     LSEAREYAVQQNINILRNRVNQLGVAEPVVQRQGADRIVVELPGIQDTARAKEILGAT
                     ATLEFRLVNTNVDQAAAASGRVPGDSEVKQTREGQPVVLYKRVILTGDHITDSTSSQD
                     EYNQPQVNISLDSAGGNIMSNFTKDNIGKPMATLFVEYKDSGKKDANGRAVLVKQEEV
                     INIANIQSRLGNSFRITGINNPSEARQLSLLLRAGALIAPIQIVEERTIGPTLGMQNI
                     EQGLEACLAGLLVSILFMIIFYKKFGLIATSALIANLILIVGIMSLLPGATLSMPGIA
                     GIVLTLAVAVDANVLINERIKEELSNGRTVQQAIDEGYRGAFSSIFDANITTLIKVII
                     LYAVGTGAIKGFAITTGIGVATSMFTAIVGTRAIVNLLYGGKRVKKLSI"
     gene            23104..24075
                     /gene="secF"
                     /locus_tag="ACHKBL_000022"
     CDS             23104..24075
                     /gene="secF"
                     /locus_tag="ACHKBL_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HFO5816953.1"
                     /translation="MAQEYTVEQLNHGRKVYDFMRWDYWAFGISGLLLIAAIVIMGVR
                     GFNWGLDFTGGTVIEITLEKPAEIDVMRDALQKAGFEEPMLQNFGSSHDIMVRMPPAE
                     GETGGQVLGSQVLKVINESTNQNAAVKRIEFVGPSVGADLAQTGAMALMAALLSILVY
                     VGFRFEWRLAAGVVIALAHDVIITLGILSLFHIEIDLTIVASLMSVIGYSLNDSIVVS
                     DRIRENFRKIRRGTPYEIFNVSLTQTLHRTLITSGTTLMVILMLYLFGGPVLEGFSLT
                     MLIGVSIGTASSIYVASALALKLGMKREHMLQQKVEKEGADQPSILP"
     gene            24205..24552
                     /gene="yajD"
                     /locus_tag="ACHKBL_000023"
     CDS             24205..24552
                     /gene="yajD"
                     /locus_tag="ACHKBL_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH nuclease YajD"
                     /protein_id="HFO5816954.1"
                     /translation="MAIIPKNYARLESGYREKALKIYPWVCGRCSREFVYSNLRELTV
                     HHIDHDHTNNPEDGSNWELLCLYCHDHEHSKYTEADQYGTTVIAGEDAQKDVGEAKYN
                     PFADLKAMMNKKK"
     gene            complement(24590..25474)
                     /gene="tsx"
                     /locus_tag="ACHKBL_000024"
     CDS             complement(24590..25474)
                     /gene="tsx"
                     /locus_tag="ACHKBL_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HFO5816955.1"
                     /translation="MKKTLLAAGAVLALSSSFTVNAAENDKPQYLSDWWHQSVNVVGS
                     YHTRFGPQLRNDTYLEYEAFAKKDWFDFYGYADAPVFFGGNSDAKGIWNHGSPLFMEI
                     EPRFSIDKLTNTDLSFGPFKEWYFANNYIYDMGRNKDGRQSTWYMGLGTDIDTGLPMS
                     LSMNVYAKYQWQNYGAANENEWDGYRFKVKYFVPITDLWGGQLSYIGFTNFDWGSDLG
                     DDSGYANNGIKTRTNNSIASSHILALNYDHWHYSVVARYWHNGGQWNDDAELNFGNGN
                     FNVRSTGWGGYLVVGYNF"
     gene            complement(25773..26312)
                     /gene="yajI"
                     /locus_tag="ACHKBL_000025"
     CDS             complement(25773..26312)
                     /gene="yajI"
                     /locus_tag="ACHKBL_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HFO5816956.1"
                     /translation="MNTNVFRLLLLGSLFSLSACVQQSEVRQMKHSVSTLNQEMTQLN
                     KETVKITQQNKLNAKSSSGVYLLPGAKTPARLESQIGTLRMSLVNITPDTDGTTLTLR
                     IQGESNDPLPAFSGTVEYGQIQGTIDNFQEINVQNQLINAPASVLAPSDVDIPLQLKG
                     MSVDQLGFVRIHDIQPVMQ"
     gene            26463..26912
                     /gene="nrdR"
                     /locus_tag="ACHKBL_000026"
     CDS             26463..26912
                     /gene="nrdR"
                     /locus_tag="ACHKBL_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HFO5816957.1"
                     /translation="MHCPFCFAVDTKVIDSRLVGEGSSVRRRRQCLVCNERFTTFEVA
                     ELVMPRVVKSNDVREPFNEEKLRSGMLRALEKRPVSSDDVEMAINHIKSQLRATGERE
                     VPSKMIGNLVMEQLKKLDKVAYIRFASVYRSFEDIKEFGEEIARLED"
     gene            26916..28019
                     /gene="ribD"
                     /locus_tag="ACHKBL_000027"
     CDS             26916..28019
                     /gene="ribD"
                     /locus_tag="ACHKBL_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HFO5816958.1"
                     /translation="MQDEYYMARALKLAQRGRFTTHPNPNVGCVIVKDGEIVGEGYHQ
                     RAGEPHAEVHALRMAGEKAKGATAYVTLEPCSHHGRTPPCCDALIAAGVARVVAAMQD
                     PNPQVAGRGLYRLQQAGIDVSHGLMMSEAEQLNKGFLKRMRTGFPYIQLKLGASLDGR
                     TAMASGESQWITSPQARRDVQRLRAQSHAILTSSATVLADDPALTVRWSELDEQTQGL
                     YPQQNLRQPVRIVIDSQNRVTPEHRIVQQPGETWFARTQEDSREWPETVRTLLIPEHK
                     GHLDLVVLMMQLGKQQINSIWVEAGPTLAGALLQAGLVDELIVYIAPKLLGNDARGLC
                     TLPGLEKLADAPQFKFKEIRHVGPDVCLHLVGA"
     gene            28108..28578
                     /gene="ribE"
                     /locus_tag="ACHKBL_000028"
     CDS             28108..28578
                     /gene="ribE"
                     /locus_tag="ACHKBL_000028"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HFO5816959.1"
                     /translation="MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQV
                     KDENITVVWVPGAYELPLAAGALAKTGKYDAVIALGTVIRGGTAHFEYVAGGASNGLA
                     HVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTALEMINVLKAIKA"
     gene            28598..29017
                     /gene="nusB"
                     /locus_tag="ACHKBL_000029"
     CDS             28598..29017
                     /gene="nusB"
                     /locus_tag="ACHKBL_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HFO5816960.1"
                     /translation="MKPAARRRARECAVQALYSWQLSQNDIADVEYQFLAEQDVKDVD
                     VLYFRELLAGVATNTAYLDGLMKPYLSRLLEELGQVEKAVLRIALYELSKRSDVPYKV
                     AINEAIELAKSFGAEDSHKFVNGVLDKAAPVIRPNKK"
     gene            29095..30072
                     /gene="thiL"
                     /locus_tag="ACHKBL_000030"
     CDS             29095..30072
                     /gene="thiL"
                     /locus_tag="ACHKBL_000030"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HFO5816961.1"
                     /translation="MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAI
                     STDTLVAGNHFLPDIDPADLAYKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLES
                     FSDSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRSGAKPGDWIYVTGT
                     PGDSAAGLAILQNRLQVADAKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLI
                     SDLGHIVKASDCGARIDLALLPFSDALSRHVEPEQALRWALSGGEDYELCFTVPELNR
                     GALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTLDWKGYDHFATP"
     gene            30050..30568
                     /gene="pgpA"
                     /locus_tag="ACHKBL_000031"
     CDS             30050..30568
                     /gene="pgpA"
                     /locus_tag="ACHKBL_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HFO5816962.1"
                     /translation="MTILPRHKDVAKSRLKMSNPWHLLAVGFGSGLSPIVPGTMGSLA
                     AIPFWYLMTFLPWQLYSLVVMLGICIGVYLCHQTAKDMGVHDHGSIVWDEFIGMWITL
                     MALPTNDWQWVTAGFVIFRILDMWKPWPIRWFDHNVHGGMGIMIDDIVAGVISAGILY
                     FIGHHWPLGILS"
     gene            complement(30622..31596)
                     /gene="yajO"
                     /locus_tag="ACHKBL_000032"
     CDS             complement(30622..31596)
                     /gene="yajO"
                     /locus_tag="ACHKBL_000032"
                     /EC_number="1.17.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxyxylulose-5-phosphate synthase YajO"
                     /protein_id="HFO5816963.1"
                     /translation="MQYNPLGKTDLRVSRLCLGCMTFGEPDRGNHAWTLPEESSRPII
                     KRALEGGINFFDTANSYSDGSSEEIVGRALRDFARREDVVVATKVFHRVGDLPEGLSR
                     AQILRSIDDSLLRLGMDYVDILQIHRWDYNTPIEETLEALNDVVKAGKARYIGASSMH
                     ASQFAQALELQKQHGWAQFVSMQDHYNLIYREEEREMLPLCYQEGVAVIPWSPLARGR
                     LTRPWGETTARLVSDEVGKNLYQESNENDAQIAERLTGVSEELGATRAQVALAWLLSK
                     PGIAAPIIGTSREEQLDELLNAVDITLKPEQIAELETPYKPHPVVGFK"
     gene            complement(31651..33513)
                     /gene="dxs"
                     /locus_tag="ACHKBL_000033"
     CDS             complement(31651..33513)
                     /gene="dxs"
                     /locus_tag="ACHKBL_000033"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HFO5816964.1"
                     /translation="MSFDIAKYPTLALVDSTQELRLLPKESLPKLCDELRRYLLDSVS
                     RSSGHFASGLGTVELTVALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKG
                     GLHPFPWRGESEYDVLSVGHSSTSISAGIGIAVAAEKEGKNRRTVCVIGDGAITAGMA
                     FEAMNHAGDIRPDMLVVLNDNEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSG
                     VPPIKELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQ
                     FLHIMTKKGRGYEPAEKDPITFHAVPKFDPSSGCLPKSSGGLPSYSKIFGDWLCETAA
                     KDNKLMAITPAMREGSGMVEFSRKFPDRYFDVAIAEQHAVTFAAGLAIGGYKPIVAIY
                     STFLQRAYDQVLHDVAIQKLPVLFAIDRAGIVGADGQTHQGAFDLSYLRCIPEIVIMT
                     PSDENECRQMLYTGYHYNDGPSAVRYPRGNAVGVELTPLEKLPIGKGIVKRRGEKLAI
                     LNFGTLMPEAAKVAESLNATLVDMRFVKPLDEALILEMAASHEALVTVEENAIMGGAG
                     SGVNEVLMAHRKPVPVLNIGLPDFFIPQGTQEEMRAELGLDATGMEAKIKAWLA"
     gene            complement(33538..34437)
                     /gene="ispA"
                     /locus_tag="ACHKBL_000034"
     CDS             complement(33538..34437)
                     /gene="ispA"
                     /locus_tag="ACHKBL_000034"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HFO5816965.1"
                     /translation="MDFPQQLEACVKQANQALSRFIAPLPFQNTPVVETMQYGALLGG
                     KRLRPFLVYATGHMFGVSTNTLDAPAAAVECIHAYSLIHDDLPAMDDDDLRRGLPTCH
                     VKFGEANAILAGDALQTLAFSILSDADMPEVSDRDRISMISELASASGIAGMCGGQAL
                     DLDAEGKHVPLEALERIHRHKTGALIRAAVRLGALSAGDKGRRALSVLDKYAESIGLA
                     FQVQDDILDVVGDTATLGKRQGADQQLGKSTYPALLGLEQARKKARDLIDDARQSLKQ
                     LAEQSLDTSALEALADYIIQRNK"
     gene            complement(34437..34679)
                     /gene="xseB"
                     /locus_tag="ACHKBL_000035"
     CDS             complement(34437..34679)
                     /gene="xseB"
                     /locus_tag="ACHKBL_000035"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HFO5816966.1"
                     /translation="MPKKNEAPASFEKALSELEQIVTRLESGDLPLEEALNEFERGVQ
                     LARQGQAKLQQAEQRVQILLSDNEDASLTPFTPDNE"
     gene            34885..36333
                     /gene="thiI"
                     /locus_tag="ACHKBL_000036"
     CDS             34885..36333
                     /gene="thiI"
                     /locus_tag="ACHKBL_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HFO5816967.1"
                     /translation="MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVV
                     RHWDNIEVRAKDENQRLAIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQL
                     EGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPDVTVHLEVEDDRLL
                     LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEI
                     GVRQVAHYLWNRFGSSHRVRFVAINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVA
                     ERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTE
                     DFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILDKVVEEANNVDIREIAQQTE
                     QEVVEVETVNGFGPNDVILDIRSIDEQEDKPLKVEGIDVVSLPFYKLSTKFGDLDQNK
                     TWLLWCERGVMSRLQALYLREQGFNNVKVYRP"
     gene            complement(36387..36977)
                     /gene="yajL"
                     /locus_tag="ACHKBL_000037"
     CDS             complement(36387..36977)
                     /gene="yajL"
                     /locus_tag="ACHKBL_000037"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase YajL"
                     /protein_id="HFO5816968.1"
                     /translation="MSASALVCLAPGSEETEAVTTIDLLVRGGIKVTTASVASDGNLA
                     ITCSRGVKLLADAPLVEVADGEYDVIVLPGGIKGAECFRDSTLLVETVKQFHRSGRIV
                     AAICAAPATVLVPHDIFPIGNMTGFPTLKDKIPAEQWQDKRVVWDARVKLLTSQGPGT
                     AIDFGLKIIDLLVGREKAHEVASQLVMAAGIYNYYE"
     gene            complement(36940..37851)
                     /gene="panE"
                     /locus_tag="ACHKBL_000038"
     CDS             complement(36940..37851)
                     /gene="panE"
                     /locus_tag="ACHKBL_000038"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFO5816969.1"
                     /translation="MKITVLGCGALGQLWLTALCKQGHDVQGWLRVPQPYCSVNLVET
                     DGSIFNESLTANDPDFLATSDLLLVTLKAWQVSDAVKSLASTLPVTTPILLIHNGMGT
                     IEELQNIQQPLLMGTTTHAARRDGNVIIHVANGITHIGPARQQDGDYSYLADILQTVL
                     PDVAWHNNIRAELWRKLAVNCVINPLTAIWNCPNGELRHHPQEIMQICEEVAAVIERE
                     GHHTSAEDLRDYVMQVIDATAENISSMLQDIRALRHTEIDYINGFLLRRARAHGITVP
                     ENTRLFEMVKRKESEYERIGTGLPRPW"
     gene            38019..38510
                     /gene="yajQ"
                     /locus_tag="ACHKBL_000039"
     CDS             38019..38510
                     /gene="yajQ"
                     /locus_tag="ACHKBL_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide binding protein YajQ"
                     /protein_id="HFO5816970.1"
                     /translation="MPSFDIVSEVDLQEARNAVDNASREVESRFDFRNVEASFELNDA
                     SKTIKVLSESDFQVNQLLDILRAKLLKRGIEGSSLDVPENIVHSGKTWFVEAKLKQGI
                     ESATQKKIVKMIKDSKLKVQAQIQGDEIRVTGKSRDDLQAVMAMVRGGDLGQPFQFKN
                     FRD"
     gene            complement(38638..40002)
                     /gene="yajR"
                     /locus_tag="ACHKBL_000040"
     CDS             complement(38638..40002)
                     /gene="yajR"
                     /locus_tag="ACHKBL_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO5816971.1"
                     /translation="MNDYKMTPGERRATWGLGTVFSLRMLGMFMVLPVLTTYGMALQG
                     ASEALIGIAIGIYGLTQAVFQIPFGLLSDRIGRKPLIVGGLAVFAAGSVIAALSDSIW
                     GIILGRALQGSGAIAAAVMALLSDLTREQNRTKAMAFIGVSFGITFAIAMVLGPIITH
                     KLGLHALFWMIAILAATGIALTIWVVPNSSTHVLNRESGMVKGSFSKVLAEPRLLKLN
                     FGIMCLHILLMSTFVALPGQMADAGFPAAEHWKVYLATMLIAFGSVVPFIIYAEVKRK
                     MKQVFVFCVGLIVVAEIVLWNAQTQFWQLVVGVQLFFVAFNLMEALLPSLISKESPAG
                     YKGTAMGVYSTSQFLGVAIGGSLGGWIDGMFDGQGVFLAGAMLAAVWLAVASTMKEPP
                     YVSSLRIEIPADTAANEALEVRLLETEGVKEVLIAEEEHSAYVKIDSKVTNRFEVEQA
                     IRQA"
     gene            complement(40151..41041)
                     /gene="cyoE"
                     /locus_tag="ACHKBL_000041"
     CDS             complement(40151..41041)
                     /gene="cyoE"
                     /locus_tag="ACHKBL_000041"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HFO5816972.1"
                     /translation="MMFKQYLQVTKPGIIFGNLISVIGGFLLASKGSIDYPLFIYTLV
                     GVSLVVASGCVFNNYIDRDIDRKMERTKNRVLVKGLISPAVSLVYATLLGIAGFMLLW
                     FGANPLACWLGVMGFVVYVGVYSLYMKRHSVYGTLIGSLSGAAPPVIGYCAVTGEFDS
                     GAAILLAIFSLWQMPHSYAIAIFRFKDYQAANIPVLPVVKGISVAKNHITLYIIAFAV
                     ATLMLSLGGYAGYKYLVVAAAVSVWWLGMALRGYKVADDRIWARKLFGFSIIAITALS
                     VMMSVDFMVPDSHTLLAAVW"
     gene            complement(41053..41382)
                     /gene="cyoD"
                     /locus_tag="ACHKBL_000042"
     CDS             complement(41053..41382)
                     /gene="cyoD"
                     /locus_tag="ACHKBL_000042"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HFO5816973.1"
                     /translation="MSHSTDHSGASHGSVKTYMTGFILSIILTVIPFWMVMTGAASPA
                     VILGTILAMAVVQVLVHLVCFLHMNTKSDEGWNMTAFVFTVLIIAILVVGSIWIMWNL
                     NYNMMMH"
     gene            complement(41382..41996)
                     /gene="cyoC"
                     /locus_tag="ACHKBL_000043"
     CDS             complement(41382..41996)
                     /gene="cyoC"
                     /locus_tag="ACHKBL_000043"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HFO5816974.1"
                     /translation="MATDTLTHATAHAHEHGHHDAGGTKVFGFWIYLMSDCILFSILF
                     ATYAVLVNGTAGGPTGKDIFELPFVLVETFLLLFSSITYGMAAIAMYKNNKSQVVSWL
                     ALTWLFGAGFIGMELYEFHHLIVNGMGPDRSGFLSAFFALVGTHGLHVTSGLIWMAVL
                     MVQIARRGLTSTNRTRIMCLSLFWHFLDVVWICVFTVVYLMGAM"
     gene            complement(41986..43977)
                     /gene="cyoB"
                     /locus_tag="ACHKBL_000044"
     CDS             complement(41986..43977)
                     /gene="cyoB"
                     /locus_tag="ACHKBL_000044"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
                     /protein_id="HFO5816975.1"
                     /translation="MFGKLSLDAVPFHEPIVMVTIAGIILGGLALVGLITYFGKWTYL
                     WKEWLTSVDHKRLGIMYIIVAIVMLLRGFADAIMMRSQQALASAGEAGFLPPHHYDQI
                     FTAHGVIMIFFVAMPFVIGLMNLVVPLQIGARDVAFPFLNNLSFWFTVVGVILVNVSL
                     GVGEFAQTGWLAYPPLSGIEYSPGVGVDYWIWSLQLSGIGTTLTGINFFVTILKMRAP
                     GMTMFKMPVFTWASLCANVLIIASFPILTVTVALLTLDRYLGTHFFTNDMGGNMMMYI
                     NLIWAWGHPEVYILILPVFGVFSEIAATFSRKRLFGYTSLVWATVCITVLSFIVWLHH
                     FFTMGAGANVNAFFGITTMIIAIPTGVKIFNWLFTMYQGRIVFHSAMLWTIGFIVTFS
                     VGGMTGVLLAVPGADFVLHNSLFLIAHFHNVIIGGVVFGCFAGMTYWWPKAFGFKLNE
                     TWGKRAFWFWIIGFFVAFMPLYALGFMGMTRRLSQQIDPQFHTMLMIAASGAVLIALG
                     ILCLVIQMYVSIRDRDQNRDLTGDPWGGRTLEWATSSPPPFYNFAVVPHVHERDAFWE
                     MKEKGEAYKKPDHYEEIHMPKNSGAGIVIAAFSTIFGFAMIWHIWWLAIVGFAGMIIT
                     WIVKSFDEDVDYYVPVAEIEKLENQHFDEITKAGLKNGN"
     gene            complement(43999..44946)
                     /gene="cyoA"
                     /locus_tag="ACHKBL_000045"
     CDS             complement(43999..44946)
                     /gene="cyoA"
                     /locus_tag="ACHKBL_000045"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit II"
                     /protein_id="HFO5816976.1"
                     /translation="MRLRKYNKSLGWLSLFAGTVLLSGCNSALLDPKGQIGLEQRSLI
                     LTAFGLMLIVVIPAILMAVGFAWKYRASNKDAKYSPNWSHSNKVEAVVWTVPILIIIF
                     LAVLTWKTTHALEPSKPLAHDEKPITIEVVSMDWKWFFIYPEQGIATVNEIAFPANTP
                     VYFKVTSNSVMNSFFIPRLGSQIYAMAGMQTRLHLIANEPGTYDGISASYSGPGFSGM
                     KFKAIATPDRAAFDQWVAKAKQSPNSMSDMAAFEKLAAPSEYNQVEYFSNVKPDLFAD
                     VINKFMAHGKSMDMTQPEGEHSAHEGMEGMDMSHAESAH"
     gene            complement(45406..46881)
                     /gene="ampG"
                     /locus_tag="ACHKBL_000046"
     CDS             complement(45406..46881)
                     /gene="ampG"
                     /locus_tag="ACHKBL_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="muropeptide MFS transporter AmpG"
                     /protein_id="HFO5816977.1"
                     /translation="MSSQYLRIFQQPRSAILLILGFASGLPLALTSGTLQAWMTVENI
                     DLKTIGFFSLVGQAYVFKFLWSPLMDRYTPPFFGRRRGWLLATQILLLVAIAAMGFLE
                     PGTQLRWMAALAVVIAFCSASQDIVFDAWKTDVLPAEERGAGAAISVLGYRLGMLVSG
                     GLALWLADKWLGWQGMYWLMAALLIPCIIATLLAPEPTDTIPVPKTLEQAVVAPLRDF
                     FGRNNAWLILLLIVLYKLGDAFAMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGA
                     LYGGILMQRLSLFRALLIFGILQGASNAGYWLLSITDKHLYSMGAAVFFENLCGGMGT
                     SAFVALLMTLCNKSFSATQFALLSALSAVGRVYVGPVAGWFVEAHGWSTFYLFSVAAA
                     VPGLILLLVCRQTLEYTRVNDNFISRTEYPAGYAFAMWTLAAGVSLLAVWLLLLTMDA
                     LDLTHFSFLPALLEVGVLVALSGVVLGGLLDYLALRKTHLT"
     gene            complement(46925..47503)
                     /gene="yajG"
                     /locus_tag="ACHKBL_000047"
     CDS             complement(46925..47503)
                     /gene="yajG"
                     /locus_tag="ACHKBL_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFO5816978.1"
                     /translation="MFKKILFPLVALFMLAGCAKPPTTIEVSPTITLPQQDPSLMGVT
                     VSINGADQRTDQALAKVTRDNQIVTLTASRDLRFLLQEVLEKQMTARGYMVGPNGPVN
                     LQIIVSQLYADVSQGNVRYNIATKADIAIIATAQNGNKMTKNYRASYNVEGAFQASNK
                     NIADAVNSVLTDTIADMSQDTSIHEFIKQNAR"
     gene            47808..48125
                     /gene="bolA"
                     /locus_tag="ACHKBL_000048"
     CDS             47808..48125
                     /gene="bolA"
                     /locus_tag="ACHKBL_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BolA"
                     /protein_id="HFO5816979.1"
                     /translation="MMIRERIEEKLRAAFQPVFLEVVDESYRHNVPAGSESHFKVVLV
                     SDRFTGERFLNRHRMIYSTLAEELSTTVHALALHTYTIKEWEGLQDTVFASPPCRGAG
                     SIA"
     gene            48469..49767
                     /gene="tig"
                     /locus_tag="ACHKBL_000049"
     CDS             48469..49767
                     /gene="tig"
                     /locus_tag="ACHKBL_000049"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFO5816980.1"
                     /translation="MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGF
                     RKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGED
                     FTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDGAVEA
                     EDRVTIDFTGSVDGEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFTIDVTFP
                     EEYHAENLKGKAAKFAINLKKVEERELPELTAEFIKRFGVEDGSVEGLRAEVRKNMER
                     ELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKQALELPR
                     ELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNK
                     ELMDNMRNVALEEQAVEAVLAKAKVTEKETTFNELMNQQA"
     gene            50013..50636
                     /gene="clpP"
                     /locus_tag="ACHKBL_000050"
     CDS             50013..50636
                     /gene="clpP"
                     /locus_tag="ACHKBL_000050"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006809880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HFO5816981.1"
                     /translation="MSYSGERDNFAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIF
                     LTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVS
                     TICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVK
                     GRMNELMALHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN"
     gene            50762..52036
                     /gene="clpX"
                     /locus_tag="ACHKBL_000051"
     CDS             50762..52036
                     /gene="clpX"
                     /locus_tag="ACHKBL_000051"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HFO5816982.1"
                     /translation="MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLC
                     NDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNG
                     DTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVEN
                     IIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVISHRVETGSGIGFGATVKA
                     KSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALT
                     KQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMED
                     VEKVVIDESVIDGQSKPLLIYGKPEAQQASGE"
     gene            52224..54578
                     /gene="lon"
                     /locus_tag="ACHKBL_000052"
     CDS             52224..54578
                     /gene="lon"
                     /locus_tag="ACHKBL_000052"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HFO5816983.1"
                     /translation="MNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAM
                     DHDKKIMLVAQKEASTDEPGVNDLFTVGTVASILQMLKLPDGTVKVLVEGLQRARISA
                     LSDNGEHFSAKAEYLESPTIDEREQEVLVRTAISQFEGYIKLNKKIPPEVLTSLNSID
                     DPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVK
                     KQMEKSQREYYLNEQMKAIQKELGEMDDAPDENEALKRKIDAAKMPKEAKEKAEAELQ
                     KLKMMSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILDTDHYGLERVKDRI
                     LEYLAVQSRVNKIKGPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGVRDEAEIR
                     GHRRTYIGSMPGKLIQKMAKVGVKNPLFLLDEIDKMSSDMRGDPASALLEVLDPEQNV
                     AFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRLSGYTEDEKLNIAKRHLLP
                     KQIERNALKKGELTVDDSAIIGIIRYYTREAGVRGLEREISKLCRKAVKQLLLDKSLK
                     HIEINGDNLHDYLGVQRFDYGRADNENRVGQVTGLAWTEVGGDLLTIETACVPGKGKL
                     TYTGSLGEVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGI
                     AMCTALVSCLTGNPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFEN
                     KRDLEEIPDNVIADLDIHPVKRIEEVLTLALQNEPSGMQVVTAK"
     gene            54769..55059
                     /gene="hupB"
                     /locus_tag="ACHKBL_000053"
     CDS             54769..55059
                     /gene="hupB"
                     /locus_tag="ACHKBL_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013356995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein HU-beta"
                     /protein_id="HFO5816984.1"
                     /translation="MKRKRRVNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKE
                     GDDVALVGFGTFAVKERAARTGRNPQTGKEITIAAAKVPSFRAGKALKDAVN"
     gene            55251..57122
                     /gene="ppiD"
                     /locus_tag="ACHKBL_000054"
     CDS             55251..57122
                     /gene="ppiD"
                     /locus_tag="ACHKBL_000054"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFO5816985.1"
                     /translation="MMDSLRTAANSLVLKIIFGIIIVSFILTGVSGYLIGGGNNYAAK
                     VNDQEISRGQFENAFNSERNRMQQQLGDQYSELAANEGYMKTLRQQVLNRLIDEALLD
                     QYARELKLGISDEQVKQAIFATPAFQVDGKFDNSRYNGILNQMGMTADQYAQALRNQL
                     TTQQLINGVAGTDFMLKGETDELAALVVQQRVVREATIDVNALAAKQPVTEQEIASYY
                     EQNKNNFMTPEQFRVSYIKLDAATMQQPVSDADIQSYYDQHQDQFTQPQRTRYSIIQT
                     KTEDEAKAVLDELNKGGDFAALAKEKSADIISARNGGDMGWLEDATIPDELKNAGLKK
                     KGQLSGVIKSSVGFLIVRLDDIQPAKVKSLDEVRDDIAAKVKHEKALDAYYALQQKVS
                     DAASNDTESLAGAEQAAGVKATQTGWFSKDNLPEELNFKPVADAIFNGGLVGENGAPG
                     INSDIITVDGDRAFVLRISEHKPEAVKPLADVQEQVKALVQHNKAEQQAKVDAEKLLV
                     DLKAGKGAEAMQAAGLKFGEPKTLSRSGRDPISQAAFALPLPAKDKPSYGMATDMQGN
                     VVLLALDEVKQGSMPEDQKKAMVQGITQNNAQIVFEALMSNLRKEAKIKIGDALEQQ"
     gene            57273..57644
                     /gene="ybaV"
                     /locus_tag="ACHKBL_000055"
     CDS             57273..57644
                     /gene="ybaV"
                     /locus_tag="ACHKBL_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HFO5816986.1"
                     /translation="MKHGIKALLITLSLACAGMSHSALAAAPAAKPTTVETKAEAPAA
                     QSKAAVPAKASDEEGTRVSINNASAEELARAMNGVGLKKAQAIVSYREEYGPFKTVED
                     LKQVPGMGNSLVERNLAVLTL"
     gene            57738..58136
                     /gene="fadM"
                     /locus_tag="ACHKBL_000056"
     CDS             57738..58136
                     /gene="fadM"
                     /locus_tag="ACHKBL_000056"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain acyl-CoA thioesterase FadM"
                     /protein_id="HFO5816987.1"
                     /translation="MQTQIKVRGYHLDVYQHVNNARYLEFLEEARWDGLENSDSFQWM
                     TAHNIAFVVVNININYRRPAVLSDLLTITSQLQQLNGKSGILSQVITLEPEGQVVADA
                     LITFVCIDLKTQKALALEGELREKLEQMVK"
     gene            complement(58188..58883)
                     /gene="queC"
                     /locus_tag="ACHKBL_000057"
     CDS             complement(58188..58883)
                     /gene="queC"
                     /locus_tag="ACHKBL_000057"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HFO5816988.1"
                     /translation="MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID
                     VARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNIL
                     FLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPL
                     MWIDKAETWALADYYGKLDLVRNETLTCYNGIKGDGCGHCAACNLRANGLHHYLADKP
                     TVMAAMKQKTGLK"
     gene            complement(58948..60648)
                     /gene="ybaE"
                     /locus_tag="ACHKBL_000058"
     CDS             complement(58948..60648)
                     /gene="ybaE"
                     /locus_tag="ACHKBL_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgrR family transcriptional regulator"
                     /protein_id="HFO5816989.1"
                     /translation="MRLLNRLNQYQRLWQPSAGKPQTVTVSELAERCFCSERHVRTLL
                     RQAQEAGWLEWQAQSGRGKRGQLRFLVTPESLRNAMMEQALETGKQQDVLELAQLAPG
                     ELRTLLQPFMGGQWQNDTPTLRIPYYRPLEPLQPGFLPGRAEQHLAGQIFSGLTRFDN
                     NTQRPIGDLAHHWETSTDGLRWDFYLRSTLHWHNGDAVKASHLHQRLLLLLQLPARDQ
                     LFISVKRIEVTHPQCLTFFLHRPDYWLAHRLASYCSHLAHPQFPLIGTGPFRLTQFTA
                     ELVRLESHDYYHLRHPLLKAVEYWITPPLFEKDLGTSCRHPVQITIGKPEELQRVSQV
                     SSGISLGFCYLTLRKSPRLSLWQARKVISIIHQSGLLQTLEVGENLITASHALLPGWT
                     IPQWQVPDEVKLPKTLTLVYHLPIELHTMAERLQATLAAEGCELTIIFHNAKNWDDTT
                     LLAHADLMMGDRLIGEAPEYTLEQWLRCDPLWPHVFDAPAYAHLQSTLDAVQVMPDEE
                     NRFNALKAVFSQLMADATLTPLFNYHYRISAPPGVNGVRLTPRGWFEFTEAWLPAPSQ
                     "
     gene            60748..61566
                     /gene="cof"
                     /locus_tag="ACHKBL_000059"
     CDS             60748..61566
                     /gene="cof"
                     /locus_tag="ACHKBL_000059"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HMP-PP phosphatase"
                     /protein_id="HFO5816990.1"
                     /translation="MARLAAFDMDGTLLMPDHHLGEKTLSTLARLRERDITLTFATGR
                     HALEMQHILGALSLDAYLITGNGTRVHSLEGELLHRDDLPADVAELVLYQQWDTRASM
                     HIFNDDGWFTGKEIPALLQAFVYSGFRYQIIDVKKMPLGSVTKICFCGDHDDLTRLQI
                     QLYEALGERAHLCFSATDCLEVLPVGCNKGAALTVLTQHLGLSLRDCMAFGDAMNDRE
                     MLGNVGSGFIMGNAMPQLRAELPHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE"
     gene            61719..62177
                     /gene="decR"
                     /locus_tag="ACHKBL_000060"
     CDS             61719..62177
                     /gene="decR"
                     /locus_tag="ACHKBL_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DecR"
                     /protein_id="HFO5816991.1"
                     /translation="MLDKIDRKLLALLQQDCTLSLQALAEAVNLTTTPCWKRLKRLED
                     DGILIGKVALLDPEKIGLGLTAFVLIKTQHHSSEWYCRFVTVVTEMPEVLGFWRMAGE
                     YDYLMRVQVADMKRYDEFYKRLVNSVPGLSDVTSSFAMEQIKYTTSLPIE"
     gene            62207..63979
                     /gene="mdlA"
                     /locus_tag="ACHKBL_000061"
     CDS             62207..63979
                     /gene="mdlA"
                     /locus_tag="ACHKBL_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SmdA family multidrug ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HFO5816992.1"
                     /translation="MRLFAQLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVD
                     GVTEQHFTTGQILMWIATMVLIAVVVYLLRYVWRVLLFGASYQLAVELREDYYRQLSR
                     QHPEFYLRHRTGDLMARATNDVDRVVFAAGEGVLTLVDSLVMGCAVLIMMSTQISWQL
                     TLFALLPMPVMAIMIKRNGDALHERFKLAQAAFSSLNDRTQESLTSIRMIKAFGLEDR
                     QSALFAEDAEDTGKKNMRVARIDARFDPTIYIAIGMANLLAIGGGSWMVVQGSLTLGQ
                     LTSFMMYLGLMIWPMLALAWMFNIVERGSAAYSRIRAMLAEAPVVNDGSEPVPEGRGE
                     LDVNIRQFTYPQTDHPALENVNFALKPGQMLGICGPTGSGKSTLLSLIQRHFDVSEGD
                     IRFHDIPLTKLQLDSWRSRLAVVSQTPFLFSDTVANNIALGCPNATQQEIEHVARLAS
                     VHDDILRLPQGYDTEVGERGVMLSGGQKQRISIARALLVNAEILILDDALSAVDGRTE
                     HQILHNLRQWGQGRTVIISAHRLSALTEASEIIVMQHGHIAQRGNHDELAQQSGWYRD
                     MYRYQQLEAALDDAPEIREEAIDA"
     gene            63972..65753
                     /gene="mdlB"
                     /locus_tag="ACHKBL_000062"
     CDS             63972..65753
                     /gene="mdlB"
                     /locus_tag="ACHKBL_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SmdB family multidrug efflux ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HFO5816993.1"
                     /translation="MRSFSQLWPTLKRLLAYGSPWRKPLGIAVIMMWVAAAAEVSGPL
                     LISYFIDNMVAKNNLPLKVVAGLAAAYVGLQLFAAGLHYAQSLLFNRAAVGVVQQLRT
                     DVMDAALRQPLSEFDTQPVGQVISRVTNDTEVIRDLYVTVVATVLRSAALVGAMLVAM
                     FSLDWRMALVAIMIFPVVLVVMVIYQRYSTPIVRRVRAYLADINDGFNEIINGMSVIQ
                     QFRQQARFGERMGEASRSHYMARMQTLRLDGFLLRPLLSLFSSLILCGLLMLFGFSAS
                     GTIEVGVLYAFISYLGRLNEPLIELTTQQAMLQQAVVAGERVFELMDGPRQQYGNDDR
                     PLQSGTIDVDNVSFAYRDDNLVLKNINLSVPSRNFVALVGHTGSGKSTLASLLMGYYP
                     LTEGEIRLDGRPLSTLSHSALRQGVAMVQQDPVVLADTFLANVTLGRDISEERVWQAL
                     EIVQLAELARSMSDGIYTPLGEQGNNLSVGQKQLLALARVLVETPQILILDEATASID
                     SGTEQAIQHALAAVREHTTLVVIAHRLSTIVDANTILVLHRGQAVEQGTHQQLLAAQG
                     RYWQMYQLQLAGEELAASVREEESLSA"
     gene            65934..66272
                     /gene="glnK"
                     /locus_tag="ACHKBL_000063"
     CDS             65934..66272
                     /gene="glnK"
                     /locus_tag="ACHKBL_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HFO5816994.1"
                     /translation="MKLVTVIIKPFKLEDVREALSSIGIQGLTVTEVKGFGRQKGHAE
                     LYRGAEYSVNFLPKVKIDVAIADDQLDEVIDIVSKAAYTGKIGDGKIFVAELQRVIRI
                     RTGEADEAAL"
     gene            66302..67588
                     /gene="amtB"
                     /locus_tag="ACHKBL_000064"
     CDS             66302..67588
                     /gene="amtB"
                     /locus_tag="ACHKBL_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter AmtB"
                     /protein_id="HFO5816995.1"
                     /translation="MKIATIKTGLASLAMLPGLVMAAPAVADKADNAFMMICTALVLF
                     MTIPGIALFYGGLIRGKNVLSMLTQVTVTFALVCILWVVYGYSLAFGEGNNFFGNING
                     LMLKNIELTAVMGSIYQYIHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTL
                     SYIPIAHMVWGGGLLASHGALDFAGGTVVHINAAIAGLVGAYLIGKRVGFGKEAFKPH
                     NLPMVFTGTAILYIGWFGFNAGSAGTANEIAALAFVNTVVATAAAILGWIFGEWALRG
                     KPSLLGACSGAIAGLVGVTPACGYIGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPC
                     DVFGVHGVCGIVGCIMTGIFAASSLGGVGFAEGVTMGHQLLVQLESIAITIVWSGVVA
                     FIGYKLADLTVGLRVPEEQEREGLDVNSHGENAYNA"
     gene            complement(67637..68497)
                     /gene="tesB"
                     /locus_tag="ACHKBL_000065"
     CDS             complement(67637..68497)
                     /gene="tesB"
                     /locus_tag="ACHKBL_000065"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HFO5816996.1"
                     /translation="MSQALKNLLTLLNLEKIEEGLFRGQSEDLGLRQVFGGQVVGQAL
                     YAAKETVPEERLVHSFHSYFLRPGDSKKPIIYDVETLRDGNSFSARRVAAIQNGKPIF
                     YMTASFQAPEAGFEHQKTMPSAPAPDGLPSETQIAQSLAHLLPPVLKDKFICDRPLEV
                     RPVEFHNPLKGHVAEPHRQVWIRANGSVPDDLRVHQYLLGYASDLNFLPVALQPHGIG
                     FLEPGIQIATIDHSMWFHRPFNLNEWLLYSVESTSASSARGFVRGEFYTQDGVLVAST
                     VQEGVMRNHN"
     gene            68715..69284
                     /gene="ybaY"
                     /locus_tag="ACHKBL_000066"
     CDS             68715..69284
                     /gene="ybaY"
                     /locus_tag="ACHKBL_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaY family lipoprotein"
                     /protein_id="HFO5816997.1"
                     /translation="MKLVHMASGLAVAIALAACADKSADIQTPAPAANTSISATQPAI
                     QQPNVSGTVWIRQKVALPPDAVLTVTLSDASLADAPSKVLAQKAVRTEGKQSPFSFVL
                     PFNPADVQPNARILLSAAITVNDKLVFITDTVQPVINQGGTKADLTLVPVQQTAVPVQ
                     ASGGATATVPSTSPTQVNPSSAVPAPTQY"
     gene            complement(69315..69626)
                     /locus_tag="ACHKBL_000067"
     CDS             complement(69315..69626)
                     /locus_tag="ACHKBL_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HFO5816998.1"
                     /translation="MEKEDSFPQRVWQIVAAIPEGYVTTYGDVAKLAGSPRAARQVGG
                     VLKRLPEGSTLPWHRVVNRHGTISLTGPDLQRQRQALLAEGVMVSGSGQIDLQRYRWN
                     Y"
     gene            69789..69885
                     /gene="ffs"
                     /locus_tag="ACHKBL_000068"
     ncRNA           69789..69885
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ACHKBL_000068"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            70005..70358
                     /gene="ybaA"
                     /locus_tag="ACHKBL_000069"
     CDS             70005..70358
                     /gene="ybaA"
                     /locus_tag="ACHKBL_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HFO5816999.1"
                     /translation="MKYVDGFVVAVPADKKDAYREMAAKAAPLFKEFGALRIVECWAS
                     DVPDGKVTDFRMAVKAEENEEVVFSWIEYPSKEVRDAANQKMMSDPRMKEFGESMPFD
                     GKRMIYGGFESIIDE"
     gene            complement(70400..71950)
                     /gene="pdeB"
                     /locus_tag="ACHKBL_000070"
     CDS             complement(70400..71950)
                     /gene="pdeB"
                     /locus_tag="ACHKBL_000070"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeB"
                     /protein_id="HFO5817000.1"
                     /translation="MRTRHLVGLISGVLILSVLLPVGLSIWLAHQQVETSFIEELNTY
                     SSRVAIRANKVATQGKDALQELERWQGAACSEAHLMEMRRVSYSYRYIQEVVYIDNNV
                     PQCSSLEHESPPDTFPEPGKISKDGYRVWLTSHNDLGIIRYMVAMGTAHYVVMIDPAS
                     FIDVIPYSSWQIDAAIIGNAHNVVITSSDEIAQGIITRLQKTPGEHIENNGIIYDILP
                     FPEMNISIITWSSTKMLQKGWHRQVFIWLPLGLVIGLLAAMFVLRILRRIQSPHHRLQ
                     DAIENRDICVHYQPIVSLANGKIVGAEALARWPQTDGSWLSPDSFIPLAQQTGLSEPL
                     TLLIIRSVFEDMGDWLRQHPQQHISINLESTVLTSEKIPQLLREMINHYQVNPRQIAL
                     ELTEREFADPKTSAPIISRYREAGHEIYLDDFGTGYSSLSYLQDLDVDILKIDKSFVD
                     ALEYKNVTPHIIEMAKTLKLKMVAEGIETSKQEEWLRQHGVHYGQGWLYSKALPKEDF
                     LRWAEQHL"
     gene            complement(72114..72584)
                     /gene="ylaC"
                     /locus_tag="ACHKBL_000071"
     CDS             complement(72114..72584)
                     /gene="ylaC"
                     /locus_tag="ACHKBL_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaC family protein"
                     /protein_id="HFO5817001.1"
                     /translation="MTEIQRLLTETIESLNTREKRDNKPRFSISFIRKHPGLFIGMYV
                     AFFATLAVMLQSETLSGSVWLLVVLFILLNGFFFFDVYPRYRYEDIDVLDFRVCYNGE
                     WYNTRFVPAALVEAILNSPRVADVHKEQLQKMIVRKGELSFYDIFTLARAESTS"
     gene            complement(72700..73251)
                     /gene="maa"
                     /locus_tag="ACHKBL_000072"
     CDS             complement(72700..73251)
                     /gene="maa"
                     /locus_tag="ACHKBL_000072"
                     /EC_number="2.3.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose O-acetyltransferase"
                     /protein_id="HFO5817002.1"
                     /translation="MSTEKEKMIAGELYHSADEALSRDRLRARRLIHRYNHSLAEEHT
                     LRQQILADLFGQVTEAYIEPTFRCDYGYNIFLGNNFFANFDCVMLDVCPIRIGDNCML
                     APGVHIYTATHPIDPVARNSGAELGKPVTIGNNVWIGGRAVINPGVTIGDNVVVASGA
                     VVTKDVPDNVVVGGNPARIIKKL"
     gene            complement(73424..73642)
                     /gene="hha"
                     /locus_tag="ACHKBL_000073"
     CDS             complement(73424..73642)
                     /gene="hha"
                     /locus_tag="ACHKBL_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HHA domain-containing protein"
                     /protein_id="HFO5817003.1"
                     /translation="MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFY
                     SAADHRLAELTMNKLYDKIPSSVWKFIR"
     gene            complement(73668..74042)
                     /gene="tomB"
                     /locus_tag="ACHKBL_000074"
     CDS             complement(73668..74042)
                     /gene="tomB"
                     /locus_tag="ACHKBL_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha toxicity modulator TomB"
                     /protein_id="HFO5817004.1"
                     /translation="MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAI
                     NLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYLDDTFMLFSSYGINMQDLQKWRK
                     SGNRLFRCFVNATKENPASLSC"
BASE COUNT        17982 a        18462 c        19913 g        17998 t
ORIGIN      
        1 ttcactttaa gtggaatttt ttctttacaa tcgaaattgt actagtttga tggtatgatc
       61 gctattctca tgacaccggc tttcgccgca ttgcgaccta ttggggaaaa cccacgatga
      121 cacaacctct ttttctgatc gggcctcggg gctgtggtaa aacaacggtc ggaatggccc
      181 ttgccgattc gcttaaccgt cggtttgtcg ataccgacca gtggttgcaa tcacagctca
      241 atatgacggt cgcggagatc gtcgaaaggg aagagtgggc gggattccgc gccagagaaa
      301 cggcggcgct ggaagcggta actgcgccat ccaccgttat cgctacaggc ggcggcatta
      361 ttctgacgga atttaatcgt cacttcatgc aaaataacgg gatcgtggtt tatttgtgtg
      421 cgccagtatc agtcctggtt aaccgactgc aagctgcacc ggaagaagat ttacggccaa
      481 ccttaacggg aaaaccactg agcgaagaag ttcaggaagt gctggaagaa cgcgatgcgc
      541 tatatcgcga agttgcgcat attatcatcg acgcaacaaa cgaacccagc caggtgattt
      601 ctgaaattcg cagcgctctg gcacagacga tcaattgttg attttcgagc gcctatactt
      661 aacgttcatc ccgtgaaata aggaagaacg atgccaacga aaccgcctta tcctcgtgaa
      721 gcatatatag tgacgattga aaaaggaaag ccaggacaga cggtaacctg gtaccaactc
      781 agagccgatc atcctaaacc agactcgttg atcagtgaac atccgaccgc tcaggaagcg
      841 atggatgcga aaaaacgcta tgaggaccct gacaaagagt gaccgcatca gactgctcgg
      901 aagggattct gagtgccgcc acaagggatc tgcgtcacat ttttcacaat tcatgttttt
      961 caaataatta gaatattaaa caataacaat ccattactgg aatcatttgg aatctttaca
     1021 ttatgcagtg caggtctgct gctacgcttt ttgtcatttg tagcacaagt aagtgtcagc
     1081 agtggtgctt aacacttgcc cggtaattaa cgacgaaata aaagtaaggt ggaagaacaa
     1141 tgagtgcgtc gttggcgatc ctgaccatcg gcattgtacc tatgcaggaa gttttgccgc
     1201 tcctgacaga atacattgac gaagataata tttcccatca tagcctgctg gggaagttaa
     1261 gccgcgaaga agtgatggcg gagtacgcgc cagaagcagg cgaagacacc attctcacat
     1321 tattaaatga caaccatctg gcccatgttt cgcgtcgcaa agtggagcga gacctgcaag
     1381 gtgtggttga agtgctcgat aatcagggtt acgacgtcat tatattaatg agtacagcaa
     1441 acattagtag tatgaccgcg cgtaatacga tctttcttga gccgtcgcgg atattgccac
     1501 cgctggtttc ctctattgtt gaagatcatc aggtgggggt tatcgtaccg gttgaggagt
     1561 tgctgaccgt tcaggcgcaa aaatggcaaa ttttgcagaa accgccagta ttttcattgg
     1621 gtaatcccat tcatgattcg gaacaaaaaa tcattgatgc cgggaaagaa ttactggcaa
     1681 aaggtgcaga tgtcatcatg ctggattgtt tgggatttaa ccagcgtcat cgcgatttac
     1741 tgcaaaaaca gctcgatgtt cctgtcttgc tctctaacgt attgattgca cggctggctg
     1801 cggaattact gatgtaattt tgcgtgacag ccagcgcctc tggcccctat agtgaagtag
     1861 atgttcaact accagacagg gccagtttat gcttcaaagt aatgagtact tttccggcaa
     1921 agtgaaatcg atcggctttt ccagcagcag cactggtcgc gccagcgtgg gtgttatggt
     1981 tgaaggtgaa tacaccttca gcaccgctga gcctgaagag atgacggtaa tcagtggcgc
     2041 gctgaatgtg ttactgcctg acgcgaccga ctggcaagtg tatgaagccg gttcgctgtt
     2101 taatgttccc ggtcacagcg agtttcatct gcaagttgcc gaacccacct cttatctgtg
     2161 ccgctatctg taattcctcg ccttcccctt gaacgggagg gcatttttct gaaatgtcct
     2221 ctctttaacc cataataata tttcctttgc tgggattttt tcgatttccg atatattcat
     2281 aattcatcaa ggttgatata aatatcagcg aagatctcca gatactattg cggaactggc
     2341 gacgttaaaa gataaatcag atgatgaatg gtggtgtgca ttgctgcaaa gtcgcaatga
     2401 tcatttgcgc caaacggcat tgcacaacgc acatactccg gtgttgttaa cgacattgac
     2461 tgagcctcag gagcggtcgc tggctattaa taattcacag ctggctgccg atgtgaaaac
     2521 ggcgtggtta aaagaggagc catcattact cttatttgtc gaacaacccg atctttcgct
     2581 gttacgtgat ttagtgaaaa acggggcaac gcgaaaaatt cgcagtgaag cgcgtcaccg
     2641 gcttgaggaa aaacaatgac acaacgcccc tggagcaagt tacagcataa aacgcataat
     2701 attgctgcgc taagaattat tgcccgctgt agctaacgat tatattcctg gtgtggcttc
     2761 gtacgccgga taagacgcgg caggcgtcgc atccggcatt aaaggtaaat cagcaattaa
     2821 cgttgtgctt cgccacctaa tccttcaatc aggttttgaa ttaacgctgc cagttcacca
     2881 gtcatcagga tgaaatcggc atcaaagcgc tgggcgaaat cttcacggtc gatatcttcg
     2941 ttttgatcgc gcagctcgtc gcagaacttc agacgcttga gcgaaccatc gtcgcacatc
     3001 acaaactgaa tgcgctgctg ccagtcgagc gccagtttag tcaccacttt tccggcttca
     3061 atgtggttgg tgatctcttc gctggtcaga tcttgtttct tcgcgcggat cactccgcca
     3121 tcttccagca acgatttcag ctcggcttca tcaagcagct ggaagccctg tgccgcgctg
     3181 ccggagcgaa cccattcggt cagcgtcagt tcaatcgggt tttccatgct caatggtaca
     3241 accggtaacg accccaggct tttacgcagc agtgccagcg tatcttccgc ttttttggca
     3301 ctggcgcagt ccaccataat caaaccgtta accgtgtcga tccacatcat tgtctggcta
     3361 aaacggctga aagcacgcgg tagcagagag tgcagcactt cgtctttcag cgaatctttt
     3421 tcggttttct tcagcttacg cgcctgttcc gcttccagtt tggcgatttt cgcttccagc
     3481 gcctgtttaa tcaccggaga cgggaggatt ttttcttctt tgcgcgcgca gataacaatt
     3541 tgaccattgg caacgtgcgt taacgcatcg ctgtgcgatc ccatcggagg aacccagccc
     3601 atcttcgcca tgtcctggct gccgcatggg gtaaatgcca tcgaggctag ctgtttttcc
     3661 atctcttctg cacgcaacga aatctcgcgg ctaagacggt aaaccattaa atttttgaac
     3721 cacagcatga taatttccac ggccttgtcg ttaaatttag cgggcatgat aacgaattgt
     3781 cggcggcctt gcattgccaa tccagttgtc cgtctctacg ctattgatat tgaaaaaaat
     3841 aaggagagta ccgtgcgtat aggtatcgat ttaggcggca ccaaaactga agtgattgca
     3901 ctgggcgatg caggggagca gttgtaccgc catcgtctgc ccacgccgcg tgatgattat
     3961 cggcagacta ttgaaacgat cgccacgttg gttgatatgg cggagcaggc aacagggcag
     4021 cgcggaacgg taggtatggg cattcctggt tcgatttcgc cctacaccgg cgtggtgaag
     4081 aatgccaatt caacctggct caacggtcag ccattcgata aagacttaag cgcgaggttg
     4141 cagcgggaag tgcggctggc aaatgacgcc aactgtctgg cggtttcaga agcagtagat
     4201 ggcgcggcag cgggagcgca gacggtattt gccgtgatta tcggcacggg atgcggcgcg
     4261 ggcgtggcat tcaatgggcg ggcgcatatc ggcggcaatg gcacggcagg tgagtgggga
     4321 cacaatccgc taccgtggat ggacgaagac gaactgcgtt atcgcgagga agtcccttgt
     4381 tattgcggta aacaaggttg tattgaaacc tttatttcgg gcacgggatt tgcgacggat
     4441 tatcgtcgtt tgagcggaca tgcgctgaaa ggcagtgaaa ttatccgcct ggttgaagaa
     4501 agcgatccgg tagcggaact ggcattgcgt cgctacgagc tgcggctggc aaaatcgctg
     4561 gcacatgtcg tgaatattct cgatccggat gtgattgtcc tggggggcgg gatgagcaat
     4621 gtagaccgtt tatatcaaac ggttccgcag ttgattaaac aatttgtctt cggcggcgaa
     4681 tgtgaaacgc cggtgcgtaa ggcgaagcac ggtgattcca gcggcgtacg cggcgctgcg
     4741 tggttatggc cacaagagta aaaaaaccag gcaattagcg catcatgcct gatgcgacgc
     4801 ttgccgcgtc ttatcaggcc tacaaaatga gcagcccgta ggccggataa ggcgtttacg
     4861 ccgcatccgg caataagcaa cgcgcacggt gacagtaatc taccccagtg gtttcgccag
     4921 caccggacta tccgccgctt gctggcgctt atagcgacca tacagcagca acgacgacat
     4981 cgcagcaaac gaaagcaggg cggcaggcag cgccacgtaa ttatatgcca gccccagcgt
     5041 cagcatcata ccaccgcaat atgcgccgac ggcgctaccg aggttaaacg ctatttgccc
     5101 acctgcggca cctaataact ctccgccttt ggcattttgc agtaacaata tttgcagtgg
     5161 tgctgaaagg gcaaataatc ccgcgcaaca aataaatgca aaaataagcg acgttgtttt
     5221 catgccgccg aagaaaaaga gcatcagcag tgccagcaca ataataaagt cagttactgc
     5281 cgcaatgcgc agtggtgaat aacgtcctga aatccggcca cttagcacat tccccagcac
     5341 catccctagc ccaactaaca tcataataaa agtcatcgcc gtttccgaaa aaccggaaat
     5401 aaacatcatg tatggcttta cgtagctgaa ccaggcaaat acacctgcgt tgccaaacat
     5461 ggtggcggcg aaaattaacc acggggccgg gctgcgtaaa aagtgaaatt gttcgcgcag
     5521 ctttcctttc gcttcgtcgc gaatatccgg cacccaaaaa tagaccgatg ccatcaccgc
     5581 aatattaaaa acagcgatca ataaaaaggt gtagcgccag ctaaattcct gacttaaata
     5641 cgttcccagc ggaatgccca gcaaattggc gactgtcatc ccggaaacca tccccgccac
     5701 ggcggcggtg acttttccgg gtttgataat ttttgataac acgatcgcgc cgacgccaaa
     5761 aaatgcgcca tgcggaaagc cggataccag ccgaccaatg gcgagcatca ggtaagacga
     5821 agagagcgtg aacatggcgt tgccaatgac gcacaacgcc accagaaaca ataagatatg
     5881 tttgagtgag tagcggctgg aaaagagtgc gatgattggc gcaccgacca ccacccccag
     5941 cgcataatac gagatcatat gcccggcagc aggaatcgaa attcctacgt tatgcgccag
     6001 ctccgtgagc acgcccataa tgccaaattc ggccatcccc aaaccaaacg tgcccagagc
     6061 caaagataaa atgacttttt tcataccacc atcacaacca ccagaacggc taccaatcac
     6121 cgtagccaat aaagtgatag cttgcaggat aacgaccagc aaggccaaga gataattcgc
     6181 ccctctgtat tcattatcct gctgaatagt tatttcactg caaacgcact ttccagtttg
     6241 ctgtagccca ggccgttgat cttcttcact ttgatctgca ccggaatacg ctctttcatc
     6301 gcttcaacgt ggctaatcac accgatggtt ttgccactgg cattcagggc atccagcgca
     6361 tcaagggcgg tatccagcgt ttcgctatcc agcgtgccaa aaccttcatc aaggaacagc
     6421 gagtcaatac gcgttttatg gctgaccaga tccgaaagcg ccagcgccag cgccagactg
     6481 acgaggaaac tttcgccgcc ggaaagggta cgggtatcgc gtaccgcatc tgcctgccag
     6541 gtatcaacaa cctcgacttc cagcgcctcg ctggctttgc gctgtaacag atagcgcccg
     6601 tgcagccggg taagttgctg attagcgaga tggactaaat tatccagcgt cagcccctgg
     6661 gcaaacttgc ggaatttatc gccctctttg gaacctatta gcgaattcag atatccccag
     6721 tcctcaacct gctgcgtcat ttgagcaatt tgctgcatta aggtttgttg ttgctgacgg
     6781 ttgtctgcat cctgcttcag ctgctggcga atctcgccct gactggtggt gttttcacgc
     6841 aacttttggt gagtttgcgc taactcttgc tgaatctgct ccatcgtcac agtgagagcc
     6901 aacccgtcag gtcggtgttg ctgatgctgt gccagcgttt ctgctgtctg agtgaccagc
     6961 gtttgcgcct gacggcgctg gttttccaga ttctgcttga gctgttccag ttgcgttagt
     7021 gtttgttcat ccattagcgc cgcaaggaac gcctgctgat cgtcaaagac gctggcctgt
     7081 agcgcggtgt caaactgcgc ctgggctttt tgcagacttt gcgccgccag aacgtcctgt
     7141 tgctgtaacg tctgctgctg gctgtgtaat gcgagacatt gttcatgaac ctgccgccag
     7201 ttttccagca ccacagtttc ttcgctgtgc gggagttcat cactttgcgg caacgtttcc
     7261 agaatcggcg tcagctgctg catacggttt tgcagcgcgg ttaattcgtt ctggcgatgc
     7321 tgccagctct gcgcttcttg ctgacgcgtc gccaaccagc tctcttcttc atcttcctgt
     7381 ggcaatgtca gtgcataacc cgccaatgcc gttaagagct gctgctggcg ttgttcaatt
     7441 tgctgttgat actggataat ttgctgatta tgcgcggcaa tctgcccttg taattcatgt
     7501 cgttggctga gtaaccgcag ctggcgttcg tgctcatctt gtgcatccag ccacggttga
     7561 atatcgtcct gtggctgcaa ggtgatattg aggctggccg tgacggcttg ccattgttga
     7621 gtaagtgctt gctcatcttg tcggaggctt tgcgcttcgt tttcatcgcg ctgaagttgc
     7681 tttgttaagg cgtccagttg cccacgtagc gtcgcacctt cttcaccgag ctttttaact
     7741 tcgttttcca gcgccagtaa tcgagactga ttaacgccag gctccagcgc ctgatacgcc
     7801 tcgaccgccg ggtggctggt ggaaccacaa agtggacaag gctgacccgc ctgtaactgt
     7861 gcacgttgag cttccagcgt tttgatgcgc gcttcctgct cgcaaatagt tttcacatcg
     7921 gcaagttgct gcgtcttttc tttataacgc tggcgcattt cgttaagtgc ggcgttacgt
     7981 tgcgtctgtt cttgcgtgac attctggata gcgacctgta attgcgccag gcgtttttgt
     8041 tggggaacaa tctgtccatg cagcgcgacc aggcgctgac gcagcgtgcg ttgctcagca
     8101 tgttgcgcca gggcggtagc aacttcatcg gcggttaacg tcaacgtgat cgccgtaagc
     8161 gcattaagtt tttgctcagc atgggttaac tgttgctgcc attgccgcag atgctcgcga
     8221 acgctggttt gttgggagaa ctgcgcacgc caacccgcca gttcgttgtt ccactgacgg
     8281 aagcggtcgt gttcctgtaa ccaggcattc aggctttgtt gctgctgctg taattctgct
     8341 gactgcttcg ccgcgtggtg gcgaatgctc gcccgaagcg ccattgtgct ctgtaagcga
     8401 gtatttactt ctttaatctg ctggcgagta tgcgccagcg ccgcgctgtg ttctgcgatg
     8461 cgttcccagt gcggacgaag atttcgtgcc ggttgtgcca gactgagcgc cgccagttga
     8521 ggttgcgctt tttcttcttc ggctaacgcc tgttgcaagg cctgctgacg gcggctggct
     8581 tcttgctgca attcgtccag acgcgttaac cagtttagtg attgttgttc ttgctgctgc
     8641 gcggtaagta actgtttttc ttcgtcagta agtacctgca aactcgctgt cagcgattgc
     8701 acttgttccg gcgtgagcaa cgcgacgccg ctggcctgcg cttgcagctt ctctagctca
     8761 gtgcgggccg atttgtgctg ctcaaacacc atcgacgaga tttgtccgta gatttcagtg
     8821 ccggttaact cctcgagcaa ttccgcgcgt tctttgggtt tggcattcag gaaggcagca
     8881 aattgcccct gcgaaagcag catcgaacgg gtgaagcgcc cgtaatccag tccggttaac
     8941 gtcgccgtca gttccagctt atctttcact ttgtcggcga gaattttgcc atcggcgcag
     9001 cgcgccagct ctacgcgtgg cacctgcaaa ttaccgtcgg gctggttacg cgcccgattc
     9061 tggctccaga acgcgcgata cgcttcacct ttcacttcaa actccacctc cgccagacat
     9121 tcggcggtat cgcgggtcat gagatcattt tgcgattgtg aaacgttaga aaggcgcgga
     9181 gtttcgtgat acagcgccag acaaatggcg tccagcaggg tggttttccc cgcaccggtt
     9241 gggccggtaa tggcaaacag cccgttgctg gcgaacggct cgcgggtgaa atcaatcttc
     9301 cattcgcctt ttaatgagtt caggtttttc aggcgcaggc tgagaatttt catgcttcgt
     9361 gttctccggc gaggctatgc aacgtcgtgg cgaaaagatg ctgcagacgt tgctgctgcg
     9421 attcctccag ttcttccagt gccagacggc gattgaacac ctcttcgacg ctgagttcgc
     9481 tgagggtttc acgctgttgg ctggctaaca cacgctcgcg ctgttcacga ctccggcgta
     9541 ccagcaatac ttcgacaggc aatgattcgg ttaatgcctg gattttgcgc tgaatatcat
     9601 gcagatactc atcagtagtg atttcgatat ccagccagac aggtggctcc tgtgatacat
     9661 cgcgccactg ttccagctgt tcggtaatcg acgccagatc gcctttcagc accgccatgg
     9721 gttgcgttac cggtacgttc aggttttcca cgctctctaa tttgccgttt gaaaatgtca
     9781 caagatggac atatttactc ttaccgcatt catcaaaact cagtggaatg ggggaaccgc
     9841 aatatcgaac atgttccatg ccgccaataa tctgtgcgcg gtgaatatgc ccgagcgcga
     9901 tgtagtcggc tggtggaaag ttttgtgccg gaaacgcgtc cagcgtgcca atatagatgt
     9961 cacgcacggc atcactttta ctggcaccca cggtcgttaa atgtcccgtg gcgatgatgg
    10021 gcagaggctg atcgccgcgc agtttgcagg catcggcata gtgttgttga taataatcag
    10081 taatagccgc cagcaaatgc tgctgttttt caataccgtt aagccccgct tggctggtaa
    10141 taatgtcacg tgggcgtaaa aacggaatgg ggcacagtac tgcgcctggt gtcccgtcgc
    10201 gacgaggcaa gatttgcggc gcatgtccgg cgctggcgac cacggttgta ttgaggaacg
    10261 ccatgatatc gcgcgattca ttcagcgtgg cgaccgagtc atggtttcct gccagtacca
    10321 ccagatgaca gccagtttgc tgtaaattga caacaaaacg gttgtataac gtgcgggcgt
    10381 aactgggcgg cgagccggta tcgaaaacat caccggcaac aataatcgca tccacctgat
    10441 gggcttgtgc tgtctccagc agccagtcaa gaaaagcctg atgttcggct tcgcggcttt
    10501 tactgtagaa gttctggccg agatgccagt ctgaggtgtg aaggatgcgc ataacggttc
    10561 cctggcgaaa aagcatgggc gcgattatac ccaaacagat gtgccatttg cttttttctg
    10621 cgccacggaa atcaataacc tgaagatatg tgcgacgagc ttttcataaa tctgtcataa
    10681 atctgacgca taatgacgtc gcattaatga tcgcaaccta tttattacaa cagggcaaat
    10741 catggcgaga cgtattctgg tcgtagaaga tgaagctcca attcgcgaaa tggtctgctt
    10801 cgtgctcgaa caaaatggct ttcagccggt cgaagcggaa gattatgaca gtgctgtgaa
    10861 tcaactgaat gaaccctggc cggatttaat tctccttgac tggatgttac ctggcggctc
    10921 cggtatccag ttcatcaaac acctcaagcg cgagtcgatg acccgggata ttccagtggt
    10981 gatgttgacc gccagagggg aagaagaaga tcgcgtgcgc ggccttgaaa ccggcgcgga
    11041 tgactatatc accaagccgt tttcgccgaa ggagctggtg gcgcgaatca aagcggtaat
    11101 gcgccgtatt tcgccaatgg cggtggaaga ggtgatcgag atgcaggggc taagcctcga
    11161 cccgacatct caccgggtga tggcgggcga agagccactg gagatggggc cgacagaatt
    11221 taaactgctg cactttttta tgacgcatcc tgagcgcgtg tacagccgcg agcagctgtt
    11281 aaaccacgtc tggggaacta acgtgtatgt ggaagaccgc acggtcgatg tccacattcg
    11341 tcgcctgcgt aaagcactgg agcccggcgg gcatgaccgc atggtgcaga ccgtgcgcgg
    11401 tacaggatat cgtttttcaa cccgctttta acgccttgct catcggacgc agagcagggc
    11461 ttatgatttc ttaactggag tatcttacgt gctggaacgg ctgtcgtgga aaaggctggt
    11521 gctggagctg ctactttgct gcctcccggc tttcatcctg ggtgcatttt ttggttacct
    11581 gccctggttt ttgctggcat cggtaacagg actgcttatc tggcatttct ggaatttatt
    11641 gcgcctttca tggtggctgt gggtggatcg cagtatgacc ccgccaccgg ggcgtggtag
    11701 ctgggaaccg ctactatacg gcttacacca gatgcagctg cgaaataaaa aacgccgccg
    11761 cgaactgggc aatctgatta aacgctttcg tagtggcgcg gagtcgctgc ccgacgcagt
    11821 agtgctgacc acggaagagg gcggtatttt ctggtgtaac ggtctggcgc aacagattct
    11881 tggtttgcgc tggccggaag ataacgggca gaacatcctt aacctgctgc gttacccgga
    11941 gtttacgcaa tatctgaaaa cgcgtgattt ttctcgcccg cttaatctgg tgctcaacac
    12001 cgggcggcat ctggaaattc gcgttatgcc ttatacccac aaacagttgc tgatggtggc
    12061 gcgtgatgtc acgcaaatgc atcaactgga aggggcgcgg cgtaactttt ttgccaacgt
    12121 gagccatgag ttacgtacgc cattgaccgt gttacagggt tacctggaga tgatggatga
    12181 gcagccgctg gaaggcgcgg tacgcgaaaa agcgttgcac accatgcgcg agcagaccca
    12241 gcggatggaa gggttggtga agcaattgct gacgctgtcg aaaatagaag ccgcaccgac
    12301 gcatttgctc aatgaaaagg ttgatgtgcc gatgatgctg cgcgtcgttg agcgcgaggc
    12361 tcagactctg agtcagaaaa aacagacatt tacctttgag atagataacg gcctcaaggt
    12421 gtcaggcaac gaagatcagc tacgcagtgc gatttcgaac ctggtctata acgccgtgaa
    12481 tcatacgccg gaaggcacgc atatcaccgt acgctggcag cgagtgccgc acggtgccga
    12541 atttagtgtt gaagataacg gaccgggcat tgcaccggag catattccgc gcctgaccga
    12601 gcgtttttat cgcgttgata aagcgcgttc ccggcaaacc ggtggtagcg gattagggtt
    12661 agcgatcgtg aaacatgcgg tgaatcatca cgaaagtcgc ctgaatattg agagtacagt
    12721 aggaaaagtt acacgtttca gttttgttat cccggaacgt ttaattgcca aaaacagcga
    12781 ttaattcgcc tttgtcatct tttattgcca taagccagcc gatgctggct tattttcttt
    12841 gcagtcgaaa tacgggcgtt agattttaca acggttggtg attttttgtt cgcatgatta
    12901 gccatgtctt tttcattgaa atagtgtttt atactggttg gtgatttctt atcgctatat
    12961 acctctggtt tttagatccc ttcttgcttt aaaacgttat aagcgtttaa attgcgcttc
    13021 aggtgctgtc atactgactg cattaacgcg gtaaatcgaa aaactattct tcgccgcgcc
    13081 tggttgggag tatttcccgc taaaattgtt taaatatacc gctgtatcat ccccagggat
    13141 tggcacaaaa atttaacgtt acaacaccac atccacaggc agtatgattt atgacccatc
    13201 aattaagatc gcgcgatatc atcgctctgg gctttatgac atttgcgttg ttcgtcggcg
    13261 caggtaacat tattttccct ccaatggtcg gcttgcaggc aggcgaacac gtctggactg
    13321 cggcattcgg cttcctcatt actgccgttg gcctgccggt attaacggtg gtggcgctgg
    13381 caaaagttgg cggcggtgtt gacagcctca gcacgccaat cggtaaagtt gctggcgtac
    13441 tgctggcaac ggtgtgttac ctggcggtgg ggccgctgtt cgctacgccg cgtacagcta
    13501 ccgtttcttt tgaagtgggg attgcgccgc tgacgggtga ttccgcactg ccgctgttta
    13561 tttacagcct ggtctatttc gctatcgtta ttctggtttc gctctatccg ggcaagctgc
    13621 tggataccgt gggcaacttc cttgcgccgc tgaaaattat cgcgctggtc atcctgtctg
    13681 ttgccgcaat tatctggccg gcgggttcta tcagcacggc tactgaggct tatcaaaacg
    13741 ctgcgttttc taacggcttc gtcaacggct atctgaccat ggatacgctg ggcgcaatgg
    13801 tgtttggtat cgttattgtt aacgcggcgc gttctcgtgg cgttaccgaa gcgcgtctgc
    13861 tgacccgtta taccgtctgg gctggcctga tggcgggtgt tggtctgact ctgctgtacc
    13921 tggcgctgtt ccgtctgggt tcagacagcg cgtcgctggt cgatcagtct gcaaacggtg
    13981 cggcgatcct gcatgcttac gttcagcata cctttggcgg cggcggtagc ttcctgctgg
    14041 cggcgttaat cttcatcgcc tgcctggtca cggcggttgg cctgacctgt gcttgtgcag
    14101 aattcttcgc ccagtacgta ccgctctctt atcgtacgct ggtgtttatc ctcggcggct
    14161 tctcgatggt ggtgtctaac ctcggcttga gtcagctgat tcagatctct gtaccggtgc
    14221 tgaccgccat ttatccgccg tgtatcgcac tggttgtatt aagttttaca cgctcatggt
    14281 ggcataattc gtcccgcgtg attgctccgc cgatgtttat cagcctgctt tttggtattc
    14341 tcgacgggat caaagcatct gcattcagcg atatcttacc gtcctgggcg cagcgtttac
    14401 cgctggccga acaaggtctg gcgtggttaa tgccaacagt ggtgatggtg gttctggcca
    14461 ttatctggga tcgcgcggca ggtcgtcagg tgacctccag cgctcactaa atcactgaac
    14521 atttgtttta accacggggc tgcgatgccc cgtggttttt tattgtgttg atgggttagg
    14581 aattgatgga aagtaagaac aagctaaagc gtgggctaag tacccgccac atacgcttta
    14641 tggcactggg ttcagcaatt ggcaccgggt tgttttacgg ttcggcagac gccatcaaaa
    14701 tggccggtcc gagcgtgttg ttggcctata ttatcggtgg tgtcgcggcg tatatcatta
    14761 tgcgcgcgct gggggaaatg tcggtacata acccggccgc cagctctttc tcgcgttatg
    14821 cgcaggaaaa cctcggcccg ctggctggtt acattaccgg ctggacctac tgctttgaaa
    14881 tccttattgt cgccatcgcc gatgtgaccg cttttggtat ctatatgggt gtctggttcc
    14941 cgacggtgcc gcactggatt tgggtactga gcgtggtgct gatcatttgc gccgtaaacc
    15001 tgatgagcgt gaaggtattc ggtgagctgg agttctggtt ctcgttcttt aaagtcgcca
    15061 ccatcatcat catgattgtc gccggtttcg gcatcatcat ctgggggatt ggcaacggcg
    15121 ggcaaccgac cggtattcat aacctgtgga gcaacggcgg cttcttcagt aacggctggc
    15181 ttgggatggt gatgtcgttg caaatggtga tgtttgctta cggtgggatc gaaattatcg
    15241 ggattaccgc cggtgaagcg aaagatcctg agaaatcgat tccgcgtgcg attaactccg
    15301 tgccgatgcg tattctggtg ttctacgtcg gtacgctgtt cgtcattatg tctatctacc
    15361 cgtggaatca ggttggcact gccggtagcc cgttcgtact gacgttccag catatgggca
    15421 ttacctttgc cgccagcatt cttaactttg ttgtgctgac cgcttcgctg tcggcaatta
    15481 acagtgacgt atttggcgta ggccgtatgc tccacggtat ggcagagcag ggcagcgcac
    15541 caaaaatttt cagcaaaacc tcgcgtcgcg gtattccgtg ggttacggtg ctggtgatga
    15601 ctaccgcgct gctgtttgct gtgtatctga actacatcat gccggaaaac gtcttcctgg
    15661 tgatcgcttc gctggcaacc ttcgccacgg tgtgggtgtg gattatgatc ctgctgtcgc
    15721 aaatcgcctt ccgtcgccgt ttaccgccag aagaagttaa gtcgctgaaa tttaaagtac
    15781 cgggtggggt agcaaccacc atcggcggtt tgattttcct gctctttatt atcgggttga
    15841 ttggttatca cccggatact cgtatctcgc tgtatgtcgg tttcgcgtgg attgttgtgc
    15901 tgttgattgg ctggatgttt aagcgccgcc acgatcgtca gctggctgaa aaccactaat
    15961 ccttgcgttc tgtaagccgg ttcaggagga aatcctgatc cggctattcc gaaagttatc
    16021 cgcccccgtc ctcctccccc aaatatcctt cagatgatga gtgatcctgc attaggctat
    16081 ggcaaggtga tcagattttc atcacagggg aattatgatg ttaaatgcat ggcacctgcc
    16141 ggtgccccca tttgttaaac aaagcaaaga tcaactgctc attacactgt ggctgacggg
    16201 cgaagaccca ccgcagcgca ttatgctgcg tacagaacac gacaacgaag aaacgtcagt
    16261 accgatgcat aagcagcgca gtcagccgca gcctggtgtt accgcatggc gggcggcgat
    16321 cgatctctcc ggcggactac ctcggcggcg ttatagtttc aaattactgt ggcacgatcg
    16381 ccagcgttgg tttacaccgc agggcttcag ccgaatgccg ccggcacgac tggagcagtt
    16441 tgccgtcgat gtaccggata tcggcccaca atgggctgcg gatcagattt tttatcagat
    16501 cttccctgat cgtttcgccc gtagtcttcc tcgtgaagct gaacaggatc atgtctatta
    16561 ccatcacgca gccggacaag agatcatctt gcgtgactgg gatgaaccgg tcacggcgca
    16621 ggcgggcgga tcaacgttct atggcggcga tctagacggg ataagcgaaa aactgccgta
    16681 tctgaaaaaa cttggcgtga cggcgctgta tctcaatccg gtgtttaaag ctcccagcgt
    16741 acataaatac gataccgagg attatcgcca tgtcgatccg cagtttggcg gtgatggggc
    16801 gttgttgcgt ttgcgacaca atacgcagca gctgggaatg cggctggtgc tggacggcgt
    16861 gtttaaccac agtggcgatt cccatgcctg gtttgacaga cacaatcgcg ggacgggtgg
    16921 tgcttgtcac aaccccgaat cgccctggcg cgactggtac tcgtttagtg acgatggcac
    16981 ggcgctcgac tggctgggct atgccagctt gccgaaactg gattatcagt cggaaagtct
    17041 ggtgaatgaa atttatcgtg gggaagacag tatcgtccgc cactggctga aagcgccgtg
    17101 gaatatggac ggctggcggc tggatgtggt gcatatgctg ggggaagcgg gtggggcgcg
    17161 caataatttg cagcacgtag ccgggatcac cgaagcggcg aaagaaaccc agccggaagc
    17221 gtatatcgtt ggagagcatt ttggcgatgc acggcaatgg ttacaggccg atgtggaaga
    17281 tgccgccatg aactatcgcg gcttcacatt cccgttgtgg ggatttcttg ccaataccga
    17341 catctcttac gatccgcagc aaattgacgc ccaaacctgt atggcctgga tggataatta
    17401 ccgcgccgga ctttctcatc aacagcaact gcgtatgttt aatcagctcg atagccacga
    17461 tactgcgcga tttaaaacgc tgctcggtcg ggatattgcg cgcctgccgc tggcggtggt
    17521 ctggttgttc acctggcctg gtgtaccgtg catttattac ggtgatgaag tgggactgga
    17581 tggtaaaaac gatccgtttt gccgtaaacc gttcccctgg caggtggaaa agcaggatac
    17641 ggcgttattc gcgctgtacc agcgaatgat tgcgctgcgt aagaaaagtc aggcgctgcg
    17701 tcgtggcggc tgtcaggtac tgtatgcgga agataacgtg gtggtatttg tccgcgtgct
    17761 gtatcagcag cgtgtactgg tggcaatcaa ccgtggcgaa gcctgtgaag tggtgctacc
    17821 cgcgtcgccg ttgcttaatg tcgcgcaatg gcaacgcaaa gaaggtcacg ggcaactgac
    17881 agacgggatt ctggctttgc ctgccatttc ggctacggtg tggatgaatt aacgtcttat
    17941 aacgccttgc gtgacgcctg ctccatcatc cgcggataaa actgccagaa gcaggtttcg
    18001 agagcatcgt aatgggcgtc taaatcgtac caggagttac gcagggcatc cagacgtggg
    18061 cgacggcttg ccatgccgtt taacacgctc tggatgaaat ccatatcgcg atagcgcacc
    18121 aaccactgct ctgaccataa gtagttgttc agattgataa agcgtggcgg tgagtctgga
    18181 agaatagtca tcacttgctc ccgggcataa caggtaaatt cctgtagcgg aaaatcgggc
    18241 gacagctgcg accagtggcg ggacagaaag tgatcccaca tcacatccag cgtaataggc
    18301 gcgacacggc gcgtttcatt acgaaaccac tcccgcgctt cgcggacttc tggcagattg
    18361 tcggttaata cgtcgattcg ccgatgcata tgaatgccag ccacgacgtc gggcggaaaa
    18421 ctttcttcag gatttccgcg tacgaaatca gccagtaaat tgccggaaag cgagctttcc
    18481 gcgagatggg ctaaatgcag gtgagctaaa aaattcatcg attatattct atccaaaggg
    18541 gggtaaaggt tgcagggaga gcgccccggc actagactac ccgcctctta ttttagtctg
    18601 agtcagtgtc atgcgcgtta ccgatttctc ttttgaattg cccgaatccc tgattgccca
    18661 ctatcccatg cctgaacgca gtagctgtcg tttactgtcg ctggacgggc cgacgggcgc
    18721 gctgacgcac ggtactttca ccgatttact cgataagctc aaccccggcg atcttctggt
    18781 ttttaataat acccgcgtga tcccggcgcg tctgtttggg cgtaaagcca gcggcggcaa
    18841 gattgaagtg ctggttgagc ggatgctcga cgacaaacgc attcttgcgc atattcgcgc
    18901 ctcgaaagcg ccaaaacctg gcgcagaact gctgctgggc gatgacgaac gtattaacgc
    18961 aacaatgacc gcgcgccacg gcgcactgtt tgaagtcgaa tttaatgatc aacgttcggt
    19021 gctggatatt ctcaacagca tcggccatat gccgctgccg ccgtacatcg accgtccgga
    19081 cgaagatgca gaccgcgaac tttatcaaac ggtttatagc gaaaaacccg gcgcggtagc
    19141 agcaccgacc gccggactgc actttgacga acctttgctg gaaaaattac gcgccaaagg
    19201 cgtggagatg gcgtttgtga cgttgcacgt tggtgcgggc accttccagc cggtgcgcgt
    19261 cgacaccatt gaagatcaca tcatgcactc ggaatacgct gaagtgccgc aggatgtggt
    19321 agacgcggta ctggcggcga aagcgcgcgg taaccgggtg attgcggttg gtaccacctc
    19381 agtacgttcg ctggaaagtg cggctcaggc agcgaaaaac gatctcattg aaccgttctt
    19441 tgacgatacc caaatcttta tctatccggg tttccagtac aaagtggtcg atgcgctggt
    19501 gacgaacttc caccttccgg aatcgacgct gattatgctg gtttcggcat ttgctggtta
    19561 tcaacacacc atgaatgcct ataaagcggc ggtagaagag aaatatcgct tttttagtta
    19621 cggtgatgcg atgtttatca cgtacaatcc gcaggcaatt aatgagcgcg ttggggagta
    19681 attccgcggc gctggtttaa acgttggact gtttttctga cgtagtggag aaaaaatgaa
    19741 atttgaactg gacaccaccg acggtcgcgc acgccgtggc cgcctggtct ttgatcgtgg
    19801 cgtagtggaa acgccttgtt ttatgcctgt tggcacttac ggcaccgtaa aagggatgac
    19861 gccggaagaa gttgaagcca ctggcgcaca aattattctc ggtaacacct tccacctgtg
    19921 gctgcgcccg ggccaggaaa tcatgaaact gcatggcgat ttacacgatt ttatgcagtg
    19981 gaaagggccg attcttaccg actccggcgg cttccaggtc ttcagccttg gtgatattcg
    20041 taaaatcacc gaacagggcg tgcacttccg taacccgatc aacggcgacc cgattttcct
    20101 cgacccggaa aagtcgatgg agattcagta cgatcttggt tcggatatcg tcatgatctt
    20161 tgatgagtgt acgccgtatc ctgctgactg ggattacgca aaacgctcca tggagatgtc
    20221 tctgcgttgg gcgaagcgta gccgtgagcg ttttgacagt ctcggaaaca aaaatgcact
    20281 gtttggtatc attcagggca gcgtttacga agatttacgt gatatttctg ttaaaggtct
    20341 ggtagatatc ggttttgatg gctacgctgt cggcggtctg gctgtgggtg agccgaaagc
    20401 agatatgcac cgcattctgg agcatgtatg cccgcaaatt ccggcagaca aaccgcgtta
    20461 cctgatgggc gttggtaaac cagaagacct ggttgaaggc gtgcgtcgcg gtatcgatat
    20521 gtttgactgc gtaatgccaa cccgcaacgc ccgaaatggt catttgttcg tgaccgatgg
    20581 cgtggtgaaa atccgcaatg cgaagtataa gagcgatact ggcccactcg atcctgagtg
    20641 tgattgctac acctgtcgca attattcacg cgcttacttg catcatctcg accgttgcaa
    20701 cgaaatatta ggcgcgcgac tcaataccat tcataacctt cgttactacc agcgtttgat
    20761 ggcgggttta cgcaaggcta ttgaagaggg taaattagag agcttcgtaa ctgattttta
    20821 ccagcgtcag gggcgagaag taccaccttt gaacgttgat taatattaat aatgagggaa
    20881 atttaatgag cttttttatt tctgatgcgg tagcggcaac gggtgcaccg gcgcaaggta
    20941 gcccgatgtc tttgattttg atgctggtgg tattcggtct gattttctat ttcatgatcc
    21001 tgcgtccaca gcagaagcgc accaaagaac acaaaaagct gatggactcc atcgctaaag
    21061 gtgatgaagt gctgacgaac ggtggcctgg tcggtcgcgt aaccaaagta gcggaaaacg
    21121 gctacattgc tatcgcgctg aatgacacca ctgaagtagt tattaaacgt gacttcgtag
    21181 ctgccgttct gccgaaaggc accatgaagg cgctgtaatt aaaatttttc cctaagggaa
    21241 ttgccgtgtt aaaccgttat cctttgtgga agtacgtcat gctgatcgtg gtgattgtca
    21301 tcggtctgct gtatgcgctt cccaacctgt ttggtgagga tccggctgtt cagatcactg
    21361 gtgcgcgcgg agtcgccgcc agtgagcaaa cgctgatcca ggtccagaaa acgttacaag
    21421 aagaaaaaat aactgctaag tctgtggcac tggaagaggg cgctattctt gcgcgcttcg
    21481 actccactga cacccagctg cgcgctcgtg aagcattaat gggcgttatg ggtgacaaat
    21541 acgtcgtggc gcttaacctt gccccggcaa cgccgcgctg gctggcagct attcacgctg
    21601 agccgatgaa gctcggcctt gacctgcgtg gcggcgttca cttcctgatg gaagtggata
    21661 tggacaccgc gcttggcaaa ctccaggaac aaaacatcga tagcctgcgc agcgacctgc
    21721 gcgaaaaggg catcccgtat accaccgttc gtaaagaaaa caactacggc ctgagcatca
    21781 ctttccgcga tgctaaagct cgtgatgaag ccattgcgta tctgagcaag cgccatccgg
    21841 acctggtgat tagcagccag ggcagcaacc agctgcgtgc ggtaatgagc gatgctcgcc
    21901 tgagtgaagc gcgtgaatat gcggtgcagc agaacattaa tatcctgcgt aaccgtgtaa
    21961 accaacttgg cgtggcggag ccggtggttc agcgtcaggg tgctgaccgt atcgttgttg
    22021 aactgccagg tattcaggac acagcgcgtg cgaaagagat tctgggtgcg acggcaacgc
    22081 tggaattccg tctggtaaac accaacgttg atcaggccgc tgcggcatcc ggtcgcgtac
    22141 cgggcgactc tgaagtgaaa cagacccgcg aaggtcagcc ggttgtgctg tacaaacgcg
    22201 taattctgac cggtgaccat atcaccgact ccacttccag ccaggacgaa tacaaccagc
    22261 cgcaggttaa catctcgctc gatagcgcgg gtggtaacat catgtctaac ttcaccaagg
    22321 acaacatcgg caaaccgatg gcaaccctgt ttgtggagta caaagacagc ggtaagaaag
    22381 atgcaaacgg tcgtgcggtt ctggtgaaac aggaagaggt gattaatatc gccaacatcc
    22441 agtctcgtct gggtaacagc ttccgtatta ctggcatcaa taacccaagc gaagcgcgtc
    22501 agctttcact gctgctgcgt gccggtgcgt tgatcgcgcc gattcagatt gttgaagaac
    22561 gtaccattgg cccaaccctg ggtatgcaga acattgagca ggggctggag gcttgccttg
    22621 ccggtctgct ggtttctatt ctgttcatga tcatcttcta taagaagttt ggtctgattg
    22681 cgaccagtgc tctgattgcc aacttgatct tgattgtcgg tattatgtcg ctgttgccag
    22741 gcgcaacgct gagtatgcca ggtatcgcgg gtatcgtctt aacccttgcg gtagcggtcg
    22801 atgcgaacgt actgatcaac gaacgtatta aagaagagtt gagcaacgga cgtactgttc
    22861 aacaggcaat tgatgaaggt tatcgtggcg cattcagttc tatcttcgat gcgaacatca
    22921 ccacgctgat taaagtcatc atcctgtacg cagtgggtac cggggcaatt aaagggttcg
    22981 cgattactac cggtatcggt gtggcgacgt cgatgtttac cgcgattgtc ggtacgcgtg
    23041 ccatcgtaaa cctgctatat ggcggcaagc gcgtcaagaa gctgtcaatc tgaggagtgc
    23101 gatgtggcac aggaatatac tgttgaacaa ctaaaccacg gccgtaaagt ctatgacttt
    23161 atgcgctggg actactgggc ttttggcatc tctggtctgc tgttaatcgc cgctatcgtt
    23221 attatgggcg tgcgcggctt taactggggg ctggatttca ccggtggtac ggttattgaa
    23281 attacgctcg aaaaaccggc tgaaattgac gtaatgcgtg atgcattgca aaaagccggt
    23341 tttgaagagc cgatgctgca aaactttggt agcagccacg acatcatggt ccgtatgccg
    23401 cctgctgaag gcgaaaccgg cggtcaggtg ctgggcagcc aggtcctgaa ggtgattaac
    23461 gaatccacca atcagaatgc ggcagtgaag cgtattgagt tcgtcggtcc gagcgtgggg
    23521 gcagaccttg cgcaaaccgg tgcgatggcg ttgatggcgg cgctgctgtc tatcctcgtg
    23581 tacgtaggtt tccgctttga gtggcgactg gcggcagggg ttgttattgc gctggcacac
    23641 gacgttatca ttacgctggg tattttgtcg ttattccata tcgagattga cctgaccatt
    23701 gtggcatcgt tgatgtcagt tatcggttac tcgcttaacg acagtatcgt ggtatcggac
    23761 cgtattcgtg aaaacttccg caagatccgt cgcggtacgc cttacgaaat ctttaacgtg
    23821 tccttgaccc agacgctgca ccgtaccttg atcacatccg gtactacctt gatggttatc
    23881 ctgatgctgt acctcttcgg tggtccggta ctggaaggct tctcgctgac catgcttatc
    23941 ggtgtttcca tcggtactgc atcttccatc tatgtggcat cggcgctggc tctgaaactg
    24001 ggtatgaagc gcgaacacat gttgcagcag aaagtggaaa aagaaggggc ggatcagccg
    24061 tcaattctgc cttaatcaag tttcagctga tgttgaaaat cccggtcaga agatcgggat
    24121 tttttttgat gtaaggtgat tgcgaaaatc cccgcatctt gggaaactgc gcgtaaccct
    24181 acatttcatc caggtaactt tttcatggct atcatcccaa aaaactatgc gcggttagaa
    24241 agcggctatc gcgaaaaagc attaaaaatc tatccgtggg tctgcggtcg ctgttcccgc
    24301 gagtttgttt attccaacct gcgtgaactt accgttcacc acattgatca cgaccatact
    24361 aataacccgg aagatggcag taactgggaa ttactttgtc tctattgcca cgatcatgag
    24421 cattcgaaat acaccgaagc ggatcagtat ggtacgaccg ttatcgcagg ggaagatgcg
    24481 cagaaagatg tcggtgaagc gaagtacaac ccattcgctg atttgaaagc gatgatgaac
    24541 aagaagaagt gataaaaaat gccggatatg aaatccggca ttctcacaat tagaagttgt
    24601 aacctactac cagataacca ccccagccgg tagagcgaac gttgaagttg ccgttgccga
    24661 agttcagttc tgcatcgtca ttccactgac caccgttatg ccagtaacgg gcaacgacag
    24721 agtagtgcca gtgatcgtaa ttcagcgcca gaatatggct ggacgcaata gagttattgg
    24781 tacgtgtttt aataccgttg ttcgcataac cgctgtcatc gcccaggtcg gaaccccagt
    24841 cgaagttggt gaagccgatg tagctcagct ggccgcccca cagatcggta atcggcacaa
    24901 agtacttcac tttgaaacgg taaccgtccc actcgttttc gttcgctgcg ccgtagttct
    24961 gccactggta tttcgcatag acgttcatgg acaggctcat cggcagacca gtatcgatgt
    25021 cggtacccag acccatgtac caggtgctct ggcggccatc tttattacga cccatgtcgt
    25081 aaatgtagtt gttcgcgaag taccactctt tgaacggacc aaagctaagg tcagtattgg
    25141 tcagcttgtc gatggagaaa cgcggttcga tttccataaa cagcggagaa ccgtggttcc
    25201 agataccttt tgcatcggag ttaccgccga agaataccgg cgcatccgca taaccataga
    25261 agtcgaacca gtctttttta gcgaatgctt cgtactcaag gtaggtatcg ttgcggagct
    25321 gcggtccgaa acgggtgtga tagcttccga caacgttaac gctctggtgc caccagtcgg
    25381 aaagatactg cggtttgtcg ttttcagctg cgttgacagt aaaagacgaa gagagcgcca
    25441 gtaccgcacc ggctgccagt aatgtttttt tcatatgtat gccactgttt gaaaatccct
    25501 tgcgggagtg aaaaaggcgc aaattgcgtt tctaaatatt tcgtatacag aggagcctat
    25561 tataaaaatc attgctcaca aaaatatgtt tcgtttcaca gttctatcat ttacgtaatc
    25621 gattgcgttc acgtttacac acattcgggc ggggattgta ctgactttca cccttgttgc
    25681 aaacttttac tatcaatcca ggtgatgtaa gcggagtaac aaaatgacgg agaaagatgt
    25741 tgcgggacgc acaatatcgc cccgcaatac gtttactgca taacaggttg aatgtcgtgg
    25801 atacgtacaa agcctaactg atccacagac atacccttta actgtaacgg aatatcaaca
    25861 tcgctggggg cgaggacgct ggcaggtgcg ttaatcaatt gattctgcac attgatttcc
    25921 tgaaagttgt ctattgttcc ctgaatttgc ccatattcaa cggttccgct gaatgcgggt
    25981 aaagggtcat tggattcgcc ctgaatacgt agcgtcaggg tagtgccatc tgtgtcaggc
    26041 gtaatattca ccagcgacat acgtaaagta ccgatctggc tttccagtct tgctggtgtt
    26101 tttgctccag gcagaaggta aaccccactg ctggattttg cattcagctt gttttgctga
    26161 gtgattttga ccgtttcttt attgagttgc gtcatctcct ggttcagcgt gctgacgctg
    26221 tgtttcatct gtcgcacttc actttgctgc acacaggcgc taaggctgaa caggcttccc
    26281 agcaggagca gtcgaaaaac gttagtattc ataggtcgtc cctttctcgt cgcaattagc
    26341 ctaatggtag agggtaaaag cagtgaaagc atcgatcctt tatctcaaaa gcgttgcgcc
    26401 tttgttgtat cgtcagttca gggtaaaata gatttctgtt aaccacctgg tcaggacgcc
    26461 gtatgcattg cccattctgt ttcgccgtgg acactaaggt aattgactct cgtctcgtgg
    26521 gcgagggttc atccgtacgc cgccgtcggc agtgtctggt gtgtaatgaa cgtttcacca
    26581 cctttgaagt ggcggagctg gttatgccgc gtgttgtaaa aagcaacgac gtgcgtgaac
    26641 cgtttaatga agagaaatta cgtagcggaa tgctgcgggc gctggaaaaa cgtccggtga
    26701 gttccgatga cgtcgaaatg gcaatcaatc atataaaatc gcagctgcgc gccaccggtg
    26761 agcgcgaagt gccgagcaag atgattggca atctggtgat ggagcaattg aaaaagctcg
    26821 ataaagtcgc ctatatccgt tttgcctctg tctaccgcag tttcgaagat atcaaagaat
    26881 ttggcgaaga gatcgcgcgc ctggaggact aagccgtgca ggacgagtat tacatggcgc
    26941 gggcgctaaa gctggcgcaa cgaggacgtt ttaccacgca tcccaacccg aatgtcgggt
    27001 gcgtcattgt caaagatggc gaaattgtcg gtgaaggtta ccatcaacgc gcgggtgaac
    27061 cgcatgccga ggtacacgcg ttgcgcatgg ccggcgaaaa agccaaaggc gcaacggcgt
    27121 atgtcacact cgaaccctgt agccatcatg gtcgtacgcc accgtgttgt gatgcattaa
    27181 ttgctgcggg cgtggcgcgc gtggttgctg caatgcaaga ccccaatccg caggtcgctg
    27241 gacgtggact ttaccgtctg caacaggctg gcattgacgt cagccacggg ctgatgatga
    27301 gtgaagctga gcaattgaat aaaggctttc tcaaacggat gcgcaccggc tttccttata
    27361 ttcagttaaa acttggcgca tcgcttgatg gtcgcacggc gatggcgagt ggcgaaagcc
    27421 agtggatcac ttcacctcag gcgaggcgcg acgtacaacg actgcgcgcg caaagtcatg
    27481 ccattttaac cagcagcgcc acggtgctgg cggatgatcc agccttaaca gtgcgttggt
    27541 ctgaactgga tgaacaaact cagggactct acccgcaaca aaatctccgc cagccggtac
    27601 gtattgtgat tgatagccaa aatcgcgtga cgccggaaca tcgcattgtg cagcagcccg
    27661 gcgaaacctg gttcgcgcgt acgcaggaag attctcgtga gtggccggaa acggtgcgta
    27721 ccttgctgat tccagagcat aaaggtcacc tggatctggt tgtactaatg atgcaactgg
    27781 gtaaacagca gattaacagc atctgggtgg aagcggggcc aacgctcgct ggcgcattgc
    27841 tacaggcggg tttagtcgat gagctgattg tctatatcgc acctaaacta ttaggcaacg
    27901 acgcccgtgg attatgcacg ctgccagggc ttgagaaatt agccgacgct ccccaattta
    27961 aattcaaaga gatacgtcat gtaggcccgg atgtttgcct gcatttagtg ggtgcatgat
    28021 ctcccggctc gaaagggaag cagcgcacga aatattatgc taaaatccgc ccccctgcgg
    28081 ggccatactc gaacccgaag gaagaaaatg aacattattg aagctaacgt tgctaccccg
    28141 gacgctcgcg tcgccatcac catcgcgcgt ttcaacaact ttatcaatga cagcctgctg
    28201 gaaggtgcaa ttgacgcact gaaacgtatc ggtcaggtaa aagatgaaaa cattaccgtt
    28261 gtttgggtgc caggtgccta tgagctgccg ctggcggcgg gtgcactggc taaaaccggt
    28321 aaatacgacg cggtgattgc gctgggtacg gttattcgtg gtggcactgc ccactttgaa
    28381 tatgtcgctg gtggtgcaag caacggcctg gcgcatgttg cccaggacag cgaaattccg
    28441 gttgcttttg gggttctgac cactgaaagc attgaacaag cgatcgaacg tgctggcacc
    28501 aaagctggca acaaaggtgc agaagctgca ctgaccgcgc ttgaaatgat taatgtattg
    28561 aaagccatca aggcctgaaa ttagtaaggg gaaatccgtg aaacctgctg ctcgtcgccg
    28621 cgctcgtgag tgtgccgtcc aggcgctcta ctcctggcag ttgtcccaga acgacatcgc
    28681 tgatgttgaa taccagttcc tggctgaaca ggatgtaaaa gacgttgacg tcctgtactt
    28741 ccgtgagctg ctggccgggg tggcgactaa caccgcatac ctcgacggac tgatgaagcc
    28801 gtacctgtcc cgtctgctgg aagaactggg ccaggtagaa aaagcagtac tgcgcattgc
    28861 gctgtacgag ctgtctaaac gtagcgatgt gccatacaaa gtggccatta acgaagcgat
    28921 cgaactggcg aaatcgttcg gcgcagaaga cagccataag ttcgtcaacg gcgtactcga
    28981 taaagcagca cctgtgattc gccctaacaa aaagtaatat ccaggccggt agattcacgg
    29041 aagaccgttc catgatcgcc ggccttttct tttttacctg ctgaggcata acgtatggca
    29101 tgtggcgagt tctccctgat tgcccgttat tttgaccgtg taagaagttc tcgtcttgat
    29161 gtcgaactgg gcatcggcga cgattgcgct cttctcaata tccccgagaa acagaccctg
    29221 gcgatcagca ctgatacgct ggtggcgggc aaccacttcc tccctgatat cgatcctgct
    29281 gatctggctt ataaagcact ggcggtgaac ctaagcgatc tggcagcgat gggggccgat
    29341 ccggcctggc tgacgctggc attaacctta ccggacgtag acgaagcgtg gcttgagtcc
    29401 ttcagcgaca gcttgtttga tcttctcaat tattacgata tgcaactcat tggcggcgat
    29461 accacgcgtg ggccattatc aatgacgttg ggtatccacg gctttgttcc gatgggacga
    29521 gccttaacgc gctctggggc gaaaccgggt gactggatct atgtgaccgg tacaccgggc
    29581 gatagcgctg ccgggctggc gattttgcaa aaccgtttgc aggttgccga tgctaaagat
    29641 gcggactact tgatcaaacg tcatctccgt ccgtcgccgc gtattttaca ggggcaggca
    29701 ctgcgcgatc tggcaaattc agctatcgat ctctctgacg gtctgatttc cgatctcggg
    29761 catatcgtga aagccagcga ctgcggcgca cgtattgacc tggcattgct gccgttttct
    29821 gatgcgcttt ctcgccatgt tgaaccggaa caggcgctgc gttgggcgct ctctggtggt
    29881 gaagattacg agttgtgttt cacggtgccg gaactgaacc gtggcgcgct ggatgtggct
    29941 ctcggacacc tgggcgtacc gtttacctgt atcgggcaaa tgaccgccga tatcgaaggg
    30001 ctttgtttta ttcgtgacgg cgaacctgtc acgttagact ggaaaggata tgaccatttt
    30061 gccacgccat aaagatgtcg cgaaaagtcg cctgaaaatg agtaatccgt ggcatctgct
    30121 tgctgtcgga ttcggaagtg gattaagccc gatcgttcct ggcacgatgg ggtcgctggc
    30181 ggcgattccc ttctggtatc tgatgacttt tttgccctgg cagctctatt cgctggtggt
    30241 aatgctgggg atctgtatcg gcgtctatct ttgtcatcaa acggcgaaag acatgggcgt
    30301 gcacgatcac ggcagtattg tctgggacga atttattggt atgtggatta cgctcatggc
    30361 gctgccgacc aatgactggc agtgggttac cgccggattt gtgattttcc gtattctgga
    30421 tatgtggaag ccgtggccga tccgctggtt tgatcacaat gtacatggcg gcatggggat
    30481 catgattgac gatattgtcg ccggggtgat ctccgctggc atcctgtatt ttatcggtca
    30541 tcactggccg ctgggtattc tgtcgtagtt gtgcaccgat gcctgatgtg acgcttgtca
    30601 cgtctcatca ggcctgcata cctatttaaa tcctacgacc ggatgcggtt tatacggcgt
    30661 ttccagctcg gcgatctgtt ccggcttcaa ggtgatatcc accgcgttta gcagttcatc
    30721 aagctgttcc tcccgagaag ttccgataat cggtgcggca atgcccggtt tactcaacaa
    30781 ccaggccagc gcaacttgtg ctcgcgttgc accaagttct tcactgacgc ccgttaagcg
    30841 ttctgcaatt tgtgcgtcat tttcattgct ttcttggtag agatttttcc ccacttcatc
    30901 cgacaccagc cgtgcggtgg tttctcccca aggacgcgtc agacggcccc gcgccagcgg
    30961 actccatgga atcaccgcca cgccttcctg ataacacagc ggcagcatct cgcgctcttc
    31021 ttcacgataa atcagattgt agtgatcctg catactgaca aactgcgccc agccgtgctg
    31081 tttttgtagt tccagtgcct gagcaaactg cgaagcgtgc atggatgacg cgccgatata
    31141 acgtgctttc ccggctttta ccacgtcgtt cagggcttcc agcgtctctt cgatcggcgt
    31201 gttgtaatcc cagcgatgaa tttgcaggat atcgacataa tccatgccga gacggagcag
    31261 gctgtcgtcg atagagcgca aaatttgtgc acgggataat ccttccggta aatcaccaac
    31321 gcgatggaac actttggtcg caacgaccac gtcttcacga cgggcgaaat cccgcagtgc
    31381 gcgaccgacg atctcttcgc tgctgccatc ggaatagcta ttggcggtat caaagaaatt
    31441 tatgccgcct tccagtgcgc gtttaatgat gggacggctg ctctcttccg gcagtgtcca
    31501 tgcgtgatta ccgcgatctg gctcgccaaa ggtcatacag ccgaggcaaa gtcgggaaac
    31561 acgaaggtcg gtttttccta aggggttgta ttgcatgctg ccactcctgc catgaataat
    31621 ttcttaagca tagcaggagt agagtaggga ttatgccagc caggctttga ttttggcttc
    31681 cataccggtg gcatcgaggc cgagttcggc gcgcatttct tcctgagttc cttgtggaat
    31741 aaagaagtcc ggcagaccaa tgttcagcac gggtactggt ttacgatggg ccatcagcac
    31801 ttcgttcacg ccgctgcctg caccacccat aatggcgttt tcttctacgg taaccagcgc
    31861 ttcatggctg gcggccattt ccagaattaa cgcttcatca agcggtttca cgaaacgcat
    31921 atcgaccagc gtagcgttca gcgattcagc gactttcgcc gcttctggca tcagcgtacc
    31981 aaagttaagg atcgccagtt tctcaccacg acgcttcaca atgcctttgc caattggcag
    32041 tttttccagt ggcgtcagtt ccacgccaac tgcgttgccg cgcgggtagc gcaccgcgga
    32101 cgggccatcg ttatagtgat agccggtata gagcatctgg cgacattcgt tttcatcgct
    32161 cggggtcata atgacaattt ccggtatgca gcgcaggtaa gagagatcaa aagcgccctg
    32221 gtgggtttga ccgtcagcac caacaatgcc cgcacggtcg atggcgaaca ggaccggaag
    32281 cttttgaatc gccacgtcat gcagcacctg atcataggcg cgttgcagga aggtggagta
    32341 gatcgcaaca atgggtttgt agcctccaat cgccagaccc gcagcaaagg tcactgcgtg
    32401 ttgctcggca attgccacgt cgaagtagcg atccgggaat ttacgtgaaa actcgaccat
    32461 gccggaacct tcacgcatcg ccggagtaat cgccatcagc ttgttatctt tcgctgcggt
    32521 ttcgcacaac cagtcgccaa agatttttga atagcttggc aaaccgccgc tacttttcgg
    32581 cagacaaccg ctggagggat caaatttagg cacggcgtgg aaggtgattg ggtctttttc
    32641 tgccggttca taaccacgac cttttttggt catgatatgc aggaactgcg ggcctttcag
    32701 gtcgcgcatg ttcttaagcg tggtgataag ccccagcaca tcgtgaccgt ccaccgggcc
    32761 gatgtagtta aagcccagct cttcaaacaa cgtgccaggc actaccatgc ctttaatatg
    32821 ttcttcggta cgtttgagca gctctttaat gggtggcacg ccagagaaaa ctttttttcc
    32881 gccttcgcgc agtgaagagt aaagcttacc ggaaagcagc tgcgccagat ggttgttgag
    32941 cgcgccaaca ttttcggaaa tcgacatttc attgtcgttg agaaccacca gcatatcagg
    33001 acggatatcg cccgcgtgat tcatcgcttc aaacgccatg ccagcagtaa tcgcgccatc
    33061 gccaatgaca cagacggtgc ggcgattttt gccttctttc tcggcagcaa ctgcgatccc
    33121 aattccggca ctgatggagg ttgatgaatg cccgacgctt aatacgtcat attcgctttc
    33181 gccgcgccac gggaacgggt gcaggccacc tttctgacgg atggtgccga ttttgtcgcg
    33241 gcgcccggtc aaaattttat gcggataagc ctgatgcccc acatcccaga ttaactggtc
    33301 aaacggggta ttgtagacat agtgcagcgc cacggtcagt tcgaccgtgc ccagcccgga
    33361 ggcgaagtgc ccgctggaac ggctcacgct gtcgagtaaa tagcggcgca gttcgtcgca
    33421 gagtttcggt aaactctctt tcggcaacag acgtaactcc tgggtggagt cgaccagtgc
    33481 cagggtcggg tatttggcaa tatcaaaact catcaggggc ctattaatac ttattgttta
    33541 tttattacgc tggatgatgt agtccgctag cgcttccagt gccgaggtat cgagtgactg
    33601 ttcagccagt tgtttcagcg actgacgggc atcgtcgatc agatcccggg ctttcttccg
    33661 ggcttgctca agacccagaa gtgcagggta ggtactttta ccaagttgct ggtcggcacc
    33721 ctggcgtttt cccaacgttg cagtatctcc caccacatcc aggatgtcat cctgaacctg
    33781 gaaggcaagg ccgatgctct ctgcgtactt gtcgagtaca gacagagcac gacgtccttt
    33841 atctccggcg cttaatgcac caaggcgaac ggcggcgcga atcaatgcgc cggttttatg
    33901 acgatgaata cgctcaagcg cctccagagg tacgtgtttg ccttccgcgt ctaaatctaa
    33961 tgcctgacca ccgcacattc cggcaatacc gctggcgctc gccagttcag aaatcatcga
    34021 aattctgtcg cgatccgaca cttccggcat atcagcatcg cttaaaatcg agaacgccag
    34081 cgtttgtaaa gcatcgccag cgagaatcgc gttcgcttcg ccaaacttca catggcaggt
    34141 cggcaaaccg cgacgcagat cgtcatcatc catcgccggt aaatcatcat gaattaatga
    34201 gtaagcgtgg atacactcaa cggcggcagc gggtgcgtcc agcgtgtttg tgctaacgcc
    34261 gaacatatga ccggtggcat aaaccaggaa aggtcgcagg cgcttaccac ctaataatgc
    34321 gccatactgc atggtttcga ccacgggagt gttctgaaag ggcagtgggg cgataaaacg
    34381 gctcagcgcc tggttggcct gcttaacgca ggcttcgagt tgctgcggaa agtccattac
    34441 tcattgtccg gtgtaaaagg ggttagagag gcgtcttcat tgtcagacag cagaatttgc
    34501 acgcgctgtt cggcttgttg taatttggcc tgcccctgac gtgccagttg cacgccgcgt
    34561 tcgaactcgt tcagcgcctc ttccagcggc aggtcgccac tttccagacg ggttacaatc
    34621 tgttccagct cgctcagcgc cttttcaaag ctggcgggcg cctcattttt cttcggcata
    34681 atgaatgtct gactctcaat atttttcgcc ccgtcatggt aacggactca gggcaaatag
    34741 caaataacgc gcaatggtaa ggtgatgtgc gcagcaaagc gatgttagtg gtatacttcc
    34801 gcgcctggat gcagccgcag gtgtgggctg ctgtattttt ccctatacaa gtcgcttaag
    34861 gcttgccaac gaaccattgc cgccatgaag tttatcatta aattgttccc ggaaatcacc
    34921 atcaaaagcc aatctgtgcg cttgcgcttt ataaaaatcc ttaccgggaa cattcgtaac
    34981 gttttaaagc actatgatga gacgctcgct gtcgtccgcc actgggataa catcgaagtt
    35041 cgcgcaaaag atgaaaacca gcgtctggct attcgcgacg ccctgacccg tattccgggc
    35101 atccaccata ttctcgaagt cgaagacgtg ccgtttaccg acatgcacga tattttcgag
    35161 aaagcgttgg ttcagtatcg cgatcagctg gaaggcaaaa ccttctgcgt gcgcgtgaag
    35221 cgccgtggca aacatgattt tagctcgatt gatgtggaac gttacgtcgg cggcggttta
    35281 aatcagcata ttgagtccgc gcgcgtgaag ctgaccaatc cggatgtgac tgtccatctg
    35341 gaagtggaag acgatcgtct cctgctgatt aaaggtcgct acgaaggtat tggcggtttc
    35401 ccgattggta cccaggaaga tgtgctgtcg ctcatttccg gtggttttga ctccggcgtt
    35461 tccagttata tgttgatgcg tcgcggatgt cgtgtgcatt actgcttctt taaccttggc
    35521 ggcgcggcgc atgaaattgg cgtgcgtcag gtggcgcatt atctgtggaa ccgctttggc
    35581 agctcccacc gcgtgcgttt tgtcgctatt aatttcgaac ctgtcgtcgg ggaaattctc
    35641 gagaaaatcg acgacggtca gatgggtgtt atcctcaaac gtatgatggt gcgtgccgcg
    35701 tctaaagtgg ctgaacgtta cggtgtacag gcgctggtca ctggcgaagc actgggccag
    35761 gtgtccagcc agacgctgac taacctgcgc ctgattgata acgtctccga cacgctgatc
    35821 ctgcgtccgc tgatttctta cgacaaagag cacatcatca acctggctcg ccagattggc
    35881 actgaagact ttgcccgcac gatgcccgaa tattgcggtg tgatctccaa aagcccgacg
    35941 gtgaaagcgg ttaaatcgaa gattgaagcg gaagaagaga agttcgactt cagcattctc
    36001 gataaagtgg ttgaggaagc gaataacgtt gatatccgcg aaatcgccca gcagaccgag
    36061 caggaagtgg tggaagtgga aaccgtcaat ggcttcggcc cgaacgacgt gatcctcgat
    36121 atccgttcta tcgatgaaca ggaagataag ccgctgaaag tcgaagggat tgatgtggtt
    36181 tctctgccgt tctataaact gagcaccaaa tttggcgatc tcgaccagaa caaaacctgg
    36241 ctgttgtggt gtgagcgcgg ggtgatgagc cgcctgcagg cgctctatct gcgcgagcag
    36301 ggctttaaca atgtgaaggt atatcgcccg taatttgtgg tttttacgtc gcatctggtc
    36361 agatgcgacg tttgccgcat ccgccactac tcgtaataat tataaatccc tgccgccatc
    36421 accagttgtg atgccacttc atgggctttt tcacgcccaa ccagcaggtc gataattttc
    36481 agaccaaagt cgatagctgt acccggcccc tgactggtca gcaattttac ccgtgcatcc
    36541 cagacgacgc gcttgtcctg ccattgttcg gcgggtattt tgtctttcag cgtcgggaag
    36601 ccggtcatat tgccaatcgg gaagatatcg tgcgggacca gcacggtggc tggtgcggcg
    36661 caaatagccg cgacgatacg cccggaacgg tggaactgtt taacggtttc aaccagcagg
    36721 gtgctatcgc gaaaacactc tgcgccttta atgcctccag gcagcacgat cacgtcatat
    36781 tcgccatcag ctacttcgac cagcggcgca tccgccagca gcttcacgcc gcgcgagcag
    36841 gtaatcgcca ggttgccatc gctggcgacg ctggcagtgg tgactttgat accgccgcga
    36901 accagcagat cgatagtggt gacggcttca gtctcttcac taccaggggc gaggcaaacc
    36961 agtgccgatg cgctcatatt cactctcctt tctttttacc atttcaaaca ggcgggtgtt
    37021 ttccggtacg gtaatcccat gcgcgcgggc gcggcgtaag agaaaaccat tgatatagtc
    37081 gatttcggtg tggcgcagcg cgcggatatc ctgcaacatc gacgagatat tttccgctgt
    37141 ggcatcaatc acctgcatca cgtaatcacg caaatcttct gctgaagtat gatgtccttc
    37201 gcgttcgatc actgccgcga cttcctcgca tatctgcata atttcttgcg gatgatgacg
    37261 taattcaccg ttcgggcaat tccagatggc agtcagtgga ttaatcacac agttgactgc
    37321 cagcttgcgc cacagctcgg cgcgaatatt gttatgccag gcaacgtcag gcaacacggt
    37381 ttgcaaaata tccgccagat aactgtaatc gccatcctgt tgccgtgccg gaccaatatg
    37441 cgtgataccg tttgccacat gaataatgac attgccgtcg cggcgagcgg catgggtggt
    37501 ggtgcccatc agtaatggct gctgaatgtt ttgcaactct tcgatggtgc ccatgccgtt
    37561 gtgaattaac agtattggcg tagttacagg cagtgtggac gcgaggcttt tgacggcatc
    37621 ggaaacctgc catgctttca gcgtcaccag gagcagatcg ctggtggcga gaaaatcggg
    37681 atcgttggcg gtcagcgatt cgttaaatat cgaaccatct gtctcaacca gattcacgct
    37741 acaataaggt tgcggtacgc gcagccagcc ctgaacgtcg tgaccctgtt tacaaagtgc
    37801 tgtaagccat aattgcccta aggcaccgca tcccaatacg gtaattttca ttgttcctcc
    37861 tcacccgcaa ccactccggg tgttcaataa ggctatccct taattgtgca tgctgttgcg
    37921 actatgcaca attaagggat acgtcctggt gcaggactgt tggttattta actttgcagg
    37981 tattatgctt cgcatcaaaa atgaagggag aggaaaagat gccatctttc gatattgtct
    38041 ctgaagttga tcttcaggaa gcacgtaacg cggtcgataa cgcgagccgc gaagtggagt
    38101 cccgttttga cttccgtaat gttgaagcct catttgagct gaacgacgcc agcaaaacca
    38161 tcaaagtgtt gagcgagtcc gatttccagg tcaatcagtt gctggatatt ctgcgtgcca
    38221 agctgctgaa gcgcggcatt gaaggcagtt cgctggatgt accggaaaat atcgttcata
    38281 gcggtaaaac ctggtttgtg gaagcgaaac tgaaacaggg cattgagagc gcgactcaga
    38341 agaaaatcgt caaaatgatc aaagacagca aactgaaagt gcaggcgcaa attcagggcg
    38401 atgagatccg cgtaacgggt aaatctcgtg atgatttgca ggctgtcatg gcgatggtac
    38461 gtggtggcga tctcggtcag ccgttccagt tcaaaaactt ccgcgattaa tcgcgacaac
    38521 ctgcgctttg ttcatgtcag atgcggcatg aacgcctgat ttggcttaca aaggtttgta
    38581 actcaggaac ttagtaggcc tgataagcgt agcgcagcag gcaatttcgc ctcgctttta
    38641 tgcctggcga attgcctgtt caacctcaaa gcgattcgtc actttgctgt cgattttcac
    38701 atacgctgaa tgttcttctt ctgcaatcaa cacttctttg acgccttcag tttccagcaa
    38761 acgtacttct aacgcttcgt ttgcggcagt gtccgccggg atttcaatgc gcaagctgct
    38821 gacatacggc ggttctttca tggtactggc gactgccagc cacactgcgg ccagcattgc
    38881 gccagcgaga aatactccct gaccgtcaaa catgccgtca atccagccgc ccagcgaacc
    38941 gccaatcgcc acgccaagaa actggctggt ggagtaaaca cccatcgccg tacctttgta
    39001 acctgctggc gattctttac tgataagtga aggcagaagg gcttccatca aattaaacgc
    39061 cacaaagaaa agctgcacgc cgaccaccag ttgccagaac tgcgtttgtg cgttccacaa
    39121 cacaatttcc gcaaccacga tcagcccgac gcaaaagaca aagacttgct tcattttgcg
    39181 cttaacttca gcgtagataa tgaaaggcac gaccgagcca aaggcgatta acatcgttgc
    39241 caggtagacc ttccagtgtt cagccgccgg gaaccccgca tcagccatct gtccgggcag
    39301 ggcaacaaag gtcgacatca gcaaaatatg cagacacata atgccaaagt tgagtttcag
    39361 cagccgcggt tccgccagca ctttactgaa acttcctttc accattccgg actcacgatt
    39421 aagtacgtga gtgctactgt tgggcacaac ccaaatggtc aacgcaatgc cggttgctgc
    39481 cagaatagcg atcatccaga acagcgcgtg cagcccaagt ttgtgagtga tgatcgggcc
    39541 aagcaccatc gcaatggcaa aggtaatgcc aaagctcacg ccgataaacg ccatcgcttt
    39601 ggtgcggttt tgttcgcgcg tgagatcgga aagcagcgcc ataacggcag cggcaatcgc
    39661 accggagcct tgtagcgccc ggcccagaat aattccccag atggagtcag agagcgcagc
    39721 gataacgcta ccggcggcaa acactgccag cccaccgaca attaatggtt tgcgaccaat
    39781 gcggtcggaa agcaggccaa acggaatctg aaaaacggcc tgagtcagac cataaatacc
    39841 aatggcaata ccgattaatg cttcgctggc accttgcaga gccatgccgt acgtggtcag
    39901 aaccggcaga accatgaaca tgcccagcat gcgcaacgag aatacggtcc ctaaacccca
    39961 ggtcgcgcgt cgctcacctg gcgtcatttt ataatcgttc attaccacct ctgttttaaa
    40021 ttcgcgacta gtgtaaagcg gcaaacgaat ggggtaaaca tgcggatttt ttagtaaata
    40081 tttcgagagg gatatgattc taatgctaag aaaaaaggtg ccgtagcacc tttttaactg
    40141 agaggttgtg ttaccacaca gcagccagca gcgtatgcga gtccggcacc ataaaatcaa
    40201 cggacatcat caccgagagg gcagtgatgg cgatgataga gaagccgaac agcttgcgcg
    40261 cccagattct gtcatcagca actttataac cgcgcagagc catgcctaac caccagacgc
    40321 tcaccgccgc ggcgactacc agatatttat acccagcgta accgccaaga gagagcatca
    40381 gcgtggcaac ggcaaaggcg atgatataca gcgtgatgtg attcttcgcc accgaaatgc
    40441 cttttaccac tggcaatacc ggaatgtttg ccgcctggta atccttaaag cggaaaatgg
    40501 cgatggcata ggagtgaggc atctgccaca gactgaagat agcaagcagg atcgctgcgc
    40561 cactatcgaa ctcgccggtt actgcacagt agccgatcac cggcggcgca gcgccggaga
    40621 gcgaaccaat caatgtgccg tagactgagt ggcgtttcat gtacaggcta taaacgccga
    40681 cataaaccac aaagcccatc acccccagcc agcaggccag cggattcgcg ccaaaccaca
    40741 gcagcataaa gccagcaata cccagcaagg tggcgtacac cagcgagaca gcaggtgaga
    40801 tcaggccttt aaccagcacc cgattcttcg tcctttccat ctttctgtcg atatccctgt
    40861 cgatgtagtt gttaaacaca caacccgacg ccacaaccag tgacacccca accagcgtgt
    40921 agataaacag gggataatca atgctgccct ttgaggccaa caggaatccc ccaatcaccg
    40981 agatcaggtt gccaaagatg atgcctggtt tcgttacttg caggtattgc ttaaacatca
    41041 taaccgccgc tcttagtgca tcatcatgtt gtagttgagg ttccacataa tccagatgga
    41101 gcctacaacc aggatagcga tgattagcac ggtgaagaca aacgccgtca tgttccagcc
    41161 ttcatctgat ttggtattca tgtgcaggaa gcacaccaga tgcaccagaa cctgtaccac
    41221 tgccattgcc aggattgttc ccagaattac ggccggagag gcagctcctg tcatcaccat
    41281 ccagaacgga atcaccgtca gaatgatcga caggataaag cctgtcatgt aggtttttac
    41341 gctgccatgg gacgcgccgc tgtgatcggt agaatgactc attacatcgc ccccatcaga
    41401 taaacaacag tgaatacaca gatccaaacc acatccagga agtgccagaa caggctcagg
    41461 cacatgatgc gggtacgatt agtgctggtc aggccgcgac gggcgatttg caccatcagc
    41521 accgccatcc agataagacc ggaagtgacg tgcagaccgt gcgtgccgac cagcgcaaag
    41581 aacgctgaca ggaagccgct gcgatccgga cccatgccgt taacaatcag atgatggaat
    41641 tcatagagct ccatcccgat aaatccggcg ccaaacaacc aggtcaacgc cagccaggag
    41701 acaacctggc ttttgttgtt tttgtacatg gcgatagccg ccatgccgta ggtgatggag
    41761 ctgaacaaca gcaggaaagt ttcaaccaga acgaacggca gttcgaaaat gtccttacct
    41821 gtcgggccgc ctgcggtgcc gttcaccaga acggcatagg tagcaaacag gatagagaac
    41881 agaatgcagt cgctcatcag gtagatccag aatccgaaga ctttggttcc gcctgcatcg
    41941 tggtgcccgt gttcgtgcgc gtgggcagtc gcgtgcgtca aagtatcagt tgccattttt
    42001 cagccctgcc ttagtaatct catcgaaatg ctggttttcc agtttttcga tttctgccac
    42061 cggcacgtag taatccacgt cctcgtcgaa gcttttcacg atccaggtga tgatcatgcc
    42121 tgcgaagcca acaatcgcca gccaccagat atgccagatc atggcgaaac cgaagatggt
    42181 ggagaaagcc gcaatgacga tacctgcacc gctgtttttc ggcatatgaa tttcttcata
    42241 gtggtcaggc tttttgtacg cttcgccttt ctctttcatt tcccagaatg catcacgctc
    42301 gtgaacgtgc ggcacaacgg caaagttata gaacggaggc ggggaagagg ttgcccactc
    42361 cagcgtacgg ccaccccacg ggtcgccagt caggtcacgg ttctggtcgc ggtcacgaat
    42421 agaaacgtac atctgaataa cgaggcagag aatacccagc gcaatcagta ctgcaccgct
    42481 ggctgcaatc atcagcatgg tgtggaactg cgggtcaatc tgctggctca aacgacgggt
    42541 catgcccatg aagcccagcg catacagcgg cataaaggca acgaagaagc cgatgatcca
    42601 gaaccagaac gcgcgtttac cccaggtttc gttcagtttg aaaccgaacg ctttaggcca
    42661 ccagtaggtc atccctgcga agcagccgaa gaccacgccg ccgatgatca cgttatggaa
    42721 gtgcgcaatc aggaacaggc tgttatgcag aacgaagtcc gcgcccggta ctgccagcag
    42781 cacgccagtc ataccgccta ccgagaaggt gacgataaaa ccgatggtcc acagcatcgc
    42841 agaatggaac acgatgcggc cctgatacat ggtgaacagc cagttgaaga tcttcacccc
    42901 ggtcgggatg gcgataatca tagtggtgat accaaagaag gcgtttacgt tcgcgcccgc
    42961 acccatcgta aagaagtggt gcagccagac gatgaacgac agcacggtga tacagacggt
    43021 tgcccatacc agcgaggtat aaccaaacag acgtttacgc gagaaggttg ccgcaatttc
    43081 ggagaacaca ccgaaaacag gcaggatcag gatgtaaact tccgggtggc cccaggccca
    43141 aatcaggttg atgtacatca tcatgttgcc acccatatcg ttggtaaaga aatgggtgcc
    43201 cagatagcga tccagggtca acaacgcaac ggtaaccgtc agaattggga aggaagcaat
    43261 aatcagtacg ttcgcgcaca gtgatgccca ggtaaatact ggcatcttga acatggtcat
    43321 gcccggtgcg cgcatcttca gaatggtaac gaagaagttg ataccggtaa gcgttgtacc
    43381 tataccggat agctggagac tccatatcca gtaatcgaca ccgactcccg gactgtactc
    43441 tattcccgat agcggtggat aggccagcca gccggtctgc gcaaattcgc ccacgccgag
    43501 agaaacgtta accagaatca caccaacaac ggtaaaccag aagcttaggt tgttgaggaa
    43561 cgggaacgca acgtcacgcg cgccgatctg cagcggaacc accaggttca tcagaccgat
    43621 aacgaaaggc atcgccacga agaagatcat aatcacgccg tgcgcggtga agatctgatc
    43681 gtagtggtga ggtggcagga agcccgcttc gcccgccgag gcaagagcct gctggctacg
    43741 catcataatg gcgtcagcaa aaccacgcag caacatcaca atcgccacga tgatatacat
    43801 gataccgagg cgtttatggt cgacggaggt cagccactct ttccacaggt aggtccactt
    43861 accgaagtaa gtgatcaggc caacgagcgc cagacctccc aaaataatgc cagcgatcgt
    43921 aaccatgacg ataggttcat ggaacgggac tgcatcaagt gataattttc cgaacatctt
    43981 tattcttcct caaccccttt aatgggcgga ttccgcgtgg ctcatgtcca tgccttccat
    44041 accttcatgt gcgctgtgct caccttctgg ctgggtcatg tccatgctct taccgtgagc
    44101 cataaacttg ttaatcacat cggcaaacaa gtctggtttc acgttggaga aatattccac
    44161 ctggttgtat tcgctaggcg cggccagttt ttcgaacgct gccatgtcag acatgctgtt
    44221 cggcgactgt ttcgcttttg cgacccattg gtcgaatgcg gcgcgatccg gtgttgcaat
    44281 agctttgaac ttcatgcctg agaagcccgg gccgctgtag ctggcggaga taccgtcata
    44341 agtaccgggt tcgttggcga tcagatgcag gcgagtctgc ataccggcca tggcataaat
    44401 ctggctaccc agacgcggaa tgaagaagga gttcatcacg gagttggagg tcactttgaa
    44461 gtacaccgga gtgttcgccg ggaaagcgat ttcattcacg gttgcaatgc cctgttccgg
    44521 gtagatgaag aaccatttcc agtccatgga aaccacttcg atggtaatgg gcttctcgtc
    44581 gtgtgccagc ggcttgctag gctcaagagc gtgagtggtt ttccaggtca gtaccgcaag
    44641 gaagatgatg attaagatag gtaccgtcca gaccacagct tccactttat tggagtgtga
    44701 ccagttcggg ctgtacttag catctttatt gctcgcacgg tacttccagg cgaaaccaac
    44761 agccatcaag attgcgggaa taacgacaat caacatcagg ccaaatgccg tcagtatcag
    44821 tgaacgttgc tccagaccaa tctgtccttt gggatctaac agcgcagaat tacagccact
    44881 gagcaataca gtgcctgcaa ataatgacaa ccatcccaaa cttttattgt atttcctgag
    44941 tctcatttaa cgacctcaat tccacgggat ctggtggcgt ttaaagtgtg tgggcatttt
    45001 acgggaaggt tacattactg taaacatgat taaattcctg ttacttggat ttggcttaca
    45061 ggtcacatat gttgcaaaac atatcaggtt gattttaaga agctgttgca aaagggcgtt
    45121 ataacaaaag gggagtgcag aaggagtctg ggcgggcaat tggtataacc aatgcgaaat
    45181 aaaacaatta tttaacaaat aattatcatt aaggtgacaa ctgggtagtg ggaaaataaa
    45241 gactcattag ctgaatcgtg taagaaaaat agaattcata aatggcgtca ataatttcca
    45301 gacgctaagc cgcacaaaag aacaaatatt aataagcgat gctgatgttt atttagccgt
    45361 aataattacg gcgagtgatt actacggcta aataatattt acagattacg tcagatgcgt
    45421 ttttcgtagc gccagataat ccagtaaacc accaagcacg acgccagaaa gggcgactaa
    45481 aaccccgact tccagcagag caggcaggaa agagaagtgc gtcaaatcca gcgcgtccat
    45541 cgtcaacagc agtaaccaca cggccaacag gctgacgccc gccgccagtg tccacatggc
    45601 aaaggcataa cctgccggat attcggtacg ggagataaag ttatcattta ctcgtgtata
    45661 ttcaagcgtc tggcgacaaa ccagcagcaa gataagccct ggtacggcag cggcgacgga
    45721 gaatagatag aatgtcgacc agccgtgtgc ttcaacaaac caacccgcca cggggccgac
    45781 ataaactcgc cctacagcag aaagcgctga gagcagggca aattgagtag cggaaaatga
    45841 cttattacac agcgtcatta acagcgcgac aaaggctgat gtacccatcc cgccacagag
    45901 gttttcgaaa aagacggctg cgcccatgct gtagagatgc ttatcagtaa tcgacagcag
    45961 ccagtaaccg gcgttagatg caccttgtaa aatgccgaaa atcagcagtg cacggaacag
    46021 cgacaggcgc tgcatcaaaa tcccaccgta caatgcgcca acaatggtcg ccagtaagcc
    46081 aagcgttttg ttaaccacgc ctacttcacc cgcatcaaac ccgacgccgc gaatcaaaaa
    46141 cgtggttgtc aggctcatag cgaatgcgtc gcccagctta tacagcacga taagaagcaa
    46201 aataagccag gcattattgc gaccaaagaa atctcgcaga ggtgcaacaa ccgcttgttc
    46261 cagcgttttg ggcacaggaa tggtgtcggt gggttctggt gcaagcaacg tcgcgataat
    46321 acaggggatc aacagcgccg ccatcaacca gtacatgccc tgccagccca gccatttatc
    46381 tgccagccac agggctaagc cgccggaaac cagcatccct aaacggtaac ccagcacgct
    46441 gattgccgcg cccgcaccac gttcttctgc cgggagcaca tcggttttcc acgcatcgaa
    46501 gacaatatcc tgggaggcag agcaaaaagc gatcaccacc gccagtgccg ccatccagcg
    46561 gagctgggtg cctggttcga gaaaacccat cgccgcaatg gcgactaata acagaatttg
    46621 cgtggcgagc agccaaccgc gccgccgccc gaaaaatggg ggcgtgtagc ggtccatcag
    46681 tggtgaccag aggaatttaa aaacgtaagc ctggcctacc agagagaaga aaccaatggt
    46741 tttgagatcg atattctcga ccgtcatcca ggcctgtaag gtgccggacg tcagggcgag
    46801 cggtagcccg gaagcaaaac ccaggatcag caatatggct gaacgcggct gttgaaaaat
    46861 acgtaaatat tgactggaca tgggcgtcta caggcccggc ttgcaccggg ccagagggca
    46921 gaaattaacg cgcgttctgc ttgatgaatt cgtggatgct ggtgtcctga gacatatcag
    46981 cgatggtatc ggtcagcaca ctgttaaccg catcggcgat atttttgttg gaggcctgga
    47041 acgcaccttc aacgttgtag ctggcacgat agtttttggt cattttgttg ccattctgcg
    47101 cggtagcgat gatggcgata tccgctttgg tcgcgatgtt gtagcgcacg ttgccctggg
    47161 acacgtcagc atacagttgg ctaacgatga tttgcagatt aaccgggcca ttcggaccaa
    47221 ccatgtatcc acgggcggtc atttgttttt ccagcacttc ttgcagcagg aaacgcagat
    47281 cgcgggaggc ggtcagggta acgatttgat tatcgcgggt gacttttgcc agcgcctgat
    47341 cggtacgctg atcggcacca ttaatgctta cggtgacgcc catcaggctt ggatcctgct
    47401 gtggcagtgt aatcgtcggg gaaacttcaa tagttgttgg cggttttgcg catcctgcaa
    47461 gcataaacag agcaactaac gggaagagga tttttttgaa catgttcggg ctctcagaga
    47521 ctcttaagcg tgtttggtaa aaattcccgc catcataaca ttgccaacgg cgaggggaag
    47581 tgggtaaggc atgtaaattc atcatgttga cgaaataatc gcccctggta aaagaaacgc
    47641 tgatgcgagg cctgtgtttc aatctttaaa tcagtaaact tcatacgctt gacagaaaaa
    47701 ccaggacgaa acctaaatat ttgttgttaa gctgcaatgg aaacggtaaa agcggctagt
    47761 atttaaaggg atggatgaca tctcagcgtt gtcggaggag atatttcatg atgatacgtg
    47821 agcggataga agaaaaatta agggcggcgt tccaacccgt attcctcgaa gtagtggatg
    47881 aaagctatcg tcacaatgtc ccagccggct ctgaaagcca ttttaaagtt gtgctggtca
    47941 gcgatcgttt tacgggtgaa cgttttctga atcgtcatcg aatgatttac agtactttag
    48001 cggaggaact ctctactacc gttcatgcgc tggctctgca tacttacact attaaggagt
    48061 gggaagggtt gcaggacacc gtctttgcct ctcctccctg tcgtggagca ggaagcatcg
    48121 cgtaaaaacg catttgcaac tgtcggcgct tttccagtat gttgctaaag attttatgaa
    48181 aaacggcctg cgggccgttt tgttttgtct ggattttgcg ctttttgccc agcattcaga
    48241 cgaaaattgc ccgggaattg tgaaaaaata cgcgacagcg cgcaataacc gttctcgact
    48301 cataaaagtg atgccgctat aatgccgcgt cctatttgaa tgctttcggg atgattctgg
    48361 taacagggaa tgtgattgat tataagaaca tcccggttcc gcgaagccaa caacctgtgc
    48421 ttgcggggta agagttgacc gagcactgtg attttttgag gtaacaagat gcaagtttca
    48481 gttgaaacca ctcaaggcct tggccgccgt gtaacgatta ctatcgctgc tgacagcatc
    48541 gagaccgctg ttaaaagcga gctggtcaac gttgcgaaaa aagtacgtat tgacggcttc
    48601 cgcaaaggca aagtgccaat gaatatcgtt gctcagcgtt atggtgcgtc tgtacgccag
    48661 gacgttctgg gtgacctgat gagccgtaac ttcattgacg ccatcattaa agaaaaaatc
    48721 aatccggctg gcgcaccgac ttatgttccg ggcgaataca agctgggtga agacttcacc
    48781 tactctgtag agtttgaagt ttatccggaa gttgaactgc agggtctgga agcgatcgaa
    48841 gttgaaaaac cgatcgttga agtgaccgac gctgacgttg acggcatgct ggatactctg
    48901 cgtaaacagc aggcgacctg gaaagaaaaa gacggcgctg ttgaagcaga agaccgcgta
    48961 accatcgact tcaccggttc tgtagacggc gaagagttcg aaggcggtaa agcgtctgat
    49021 ttcgtactgg cgatgggcca ggggcgtatg attccgggct ttgaagacgg tatcaaaggc
    49081 cacaaagctg gcgaagagtt caccatcgac gtgaccttcc cggaagaata ccacgcagaa
    49141 aacctgaaag gtaaagcagc gaaattcgct atcaacctga agaaagttga agagcgtgaa
    49201 ctgccggaac tgactgcaga attcatcaaa cgtttcggcg ttgaagatgg ttccgtagaa
    49261 ggtctgcgcg ctgaagtgcg taaaaacatg gagcgcgagc tgaagagcgc catccgtaac
    49321 cgcgttaagt ctcaggcgat cgaaggtctg gtaaaagcta acgacatcga cgtaccggct
    49381 gcgctgatcg acagcgaaat cgacgttctg cgtcgccagg ctgcacagcg tttcggtggc
    49441 aacgaaaaac aagctctgga actgccgcgt gaactgtttg aagaacaggc taaacgtcgc
    49501 gtagttgttg gcctgctgct gggcgaagtt atccgcacca acgagctgaa agctgacgaa
    49561 gagcgcgtga aaggcctgat cgaagagatg gcttctgcgt acgaagatcc gaaagaagtt
    49621 atcgagttct acagcaaaaa caaagaactg atggacaaca tgcgcaatgt tgctctggaa
    49681 gaacaggctg ttgaagctgt actggcgaaa gcgaaagtga ctgaaaaaga aaccactttc
    49741 aacgagctga tgaaccagca ggcgtaattt acgcagcata acgcgctaaa ttcgcacaaa
    49801 ggcccgtcac cgccaggtgg tgggcttttt tttgtcatga attttgcatg gaaccgtgcg
    49861 aaaagcctct ttcggtgtta gcgtaacaac aaaagattgt tatgcttgaa atatggtgat
    49921 gccgtaccca taacacaggg actagctgat aatccgtcca taaggttaca atcggtacag
    49981 caggtttttt caattttatc caggagacgg aaatgtcata cagcggcgaa cgagataact
    50041 ttgcacccca tatggcgctg gtgccgatgg tcattgaaca gacctcacgt ggtgagcgct
    50101 cttttgatat ctattctcgt ctacttaagg aacgcgtcat ttttctgact ggccaggttg
    50161 aagaccacat ggctaacctg attgtggcgc agatgctgtt cctggaagca gaaaacccag
    50221 aaaaagatat ctatctgtac attaactccc caggcggggt gatcactgcc gggatgtcta
    50281 tctatgacac catgcagttt atcaagcctg atgtcagcac catctgtatg ggccaggcgg
    50341 cttcgatggg cgctttcttg ctgactgcag gggcaaaagg taaacgtttc tgcctgccga
    50401 attcgcgcgt gatgattcac cagccgttgg gcggctacca gggccaggcg accgatatcg
    50461 aaattcatgc ccgtgaaatt ctgaaagtta aagggcgcat gaatgaactt atggcgcttc
    50521 atacgggtca atcattagaa cagattgaac gtgataccga gcgcgatcgc ttcctttccg
    50581 cccctgaagc ggtggaatac ggtctggtcg attcgattct gacccatcgt aattgatgcc
    50641 agaggcgcaa ctgtgccgct atacttatcc agggcggcac aacgctgtaa gcggcttgcg
    50701 cctgagaatg gcatttgcgt cgtcgtgtgc ggcacaaaga acaaagaaga ggttttgacc
    50761 catgacagat aaacgcaaag atggctcagg caaattgctg tattgctctt tttgcggcaa
    50821 aagccagcat gaagtgcgca agctgattgc cggtccatcc gtgtatatct gcgacgaatg
    50881 tgttgattta tgtaacgaca tcattcgcga agagattaaa gaagttgcac cgcatcgtga
    50941 acgcagtgcg ctaccgacgc cgcatgaaat tcgcaaccac ctggacgatt acgttatcgg
    51001 ccaggaacag gcgaaaaaag tgctggcggt cgcggtatac aaccactaca aacgtctgcg
    51061 caacggcgat accagcaatg gtgtcgagtt gggcaaaagt aacattctgc tgatcggtcc
    51121 gaccggttcc ggtaaaacgc tgctggctga aacgctggcg cgcctgctgg acgtcccgtt
    51181 caccatggcc gacgcaacca cgctgaccga agccggttat gtgggcgaag acgttgaaaa
    51241 catcattcag aagctgttgc agaagtgcga ttacgacgta cagaaagcgc agcgcgggat
    51301 tgtctacatc gatgaaatcg acaagatttc tcgtaagtca gacaacccgt ccattacccg
    51361 agacgtttcc ggtgaaggcg tacagcaggc actgttgaaa ctgatcgaag gtacggtagc
    51421 tgctgttcca ccgcaaggtg ggcgtaaaca tccgcagcag gaattcttgc aggttgatac
    51481 ctctaagatc ctgtttattt gtggcggtgc gtttgccggt ctggataaag tgatttccca
    51541 tcgtgtagaa accggctccg gcattggttt tggcgcgacg gtaaaagcga agtccgacaa
    51601 agcaagcgaa ggcgagctgc tggcgcaggt tgaaccggaa gatctgatca agtttggtct
    51661 tatccctgag tttattggtc gtctgccggt tgtcgcaacg ttgaatgaac tgagcgaaga
    51721 agctctgatt cagatcctca aagagccgaa aaacgccctg accaagcagt atcaggcgct
    51781 gtttaatctg gaaggcgtgg atctggaatt ccgtgacgag gcgctggatg ctatcgctaa
    51841 gaaagcgatg gcgcgtaaaa ccggtgcccg tggcctgcgt tccatcgtag aagccgcact
    51901 gctcgatacc atgtacgatc tgccgtccat ggaagacgtc gaaaaagtgg ttatcgacga
    51961 atcggtaatt gatggtcaaa gcaagccgtt gctgatttat ggcaagccgg aagcgcaaca
    52021 ggcatctggt gaataattaa ccattcccat acaattagtt aaccaaaaag gggggatttt
    52081 atctcccctt taatttttcc tctattctcg gcgttgaatg tgggggaaac atccccatat
    52141 actgacgtac atgttaatag atggcgtgaa gcacagtcgt gtcatctgat tacctggcgg
    52201 aaattaaact aagagagagc tctatgaatc ctgagcgttc tgaacgcatt gaaatccccg
    52261 tattgccgct gcgcgatgtg gtggtttatc cgcacatggt catcccctta tttgtcgggc
    52321 gggaaaaatc tatccgttgt ctggaagcgg cgatggacca tgataaaaaa attatgctgg
    52381 tcgcgcagaa agaagcttca acggatgagc cgggtgtaaa cgatcttttc accgtcggga
    52441 ccgtggcctc tatattgcag atgctgaaac tgcctgacgg caccgtcaaa gtgctggtcg
    52501 aggggttaca gcgcgcgcgt atttctgcgc tctctgacaa tggcgaacac ttttctgcga
    52561 aggcggagta tctggagtcg ccgaccattg atgagcggga acaggaagtg ctggtgcgta
    52621 ctgcaatcag ccagttcgaa ggctacatca agctgaacaa aaaaatccca ccagaagtgc
    52681 tgacgtcgct gaatagcatc gacgacccgg cgcgtctggc ggataccatt gctgcacata
    52741 tgccgctgaa actggctgac aaacagtccg ttctggagat gtccgacgtt aacgaacgtc
    52801 tggaatatct gatggcaatg atggaatcgg aaatcgatct gctgcaggtt gagaaacgca
    52861 ttcgcaaccg cgttaaaaag cagatggaga aatcccagcg tgagtactat ctgaacgagc
    52921 aaatgaaagc tattcagaaa gaactcggtg aaatggacga cgcgccggac gaaaacgaag
    52981 ccctgaagcg caaaattgac gcggcgaaaa tgccgaaaga ggcaaaagag aaagcggaag
    53041 cagagttgca gaagctgaaa atgatgtctc cgatgtcggc agaagcgacc gtagtgcgtg
    53101 gttatatcga ctggatggta caggtgccgt ggaatgcgcg cagcaaggtc aaaaaagacc
    53161 tgcgtcaggc gcaggaaatc cttgataccg accattatgg tctggagcgc gtgaaagatc
    53221 gcatccttga gtatcttgcg gttcaaagcc gtgtcaacaa aatcaaggga ccgatcctct
    53281 gcctggtagg gccgccgggg gtaggtaaaa cctctcttgg tcagtccatt gccaaagcca
    53341 ccgggcgtaa atatgtccgt atggcgctgg gcggcgtgcg tgatgaagcg gaaatccgtg
    53401 gtcaccgccg tacttacatc ggttctatgc cgggtaaact gatccagaaa atggcgaaag
    53461 tgggcgtgaa aaacccgctg ttcctgctcg atgagatcga caaaatgtct tctgacatgc
    53521 gtggcgatcc ggcctccgca ctgcttgaag tgctggatcc agagcagaac gtagcgttca
    53581 gcgaccacta cctggaagtg gattacgacc ttagcgacgt gatgtttgtc gcgacgtcga
    53641 actccatgaa cattccggca ccgctgctgg atcgtatgga agtgattcgc ctctccggtt
    53701 ataccgaaga tgaaaaactg aacatcgcca aacgtcacct gctgccgaag cagattgaac
    53761 gtaatgcact gaaaaaaggt gagctgaccg tcgacgatag cgccattatc ggcattattc
    53821 gttactacac ccgtgaggcg ggcgtgcgtg gtctggagcg tgaaatctcc aaactgtgtc
    53881 gcaaagcggt taagcagtta ctgctcgata agtcattaaa acatatcgaa attaacggcg
    53941 ataacctgca tgactatctc ggtgttcagc gcttcgacta tggtcgcgcg gataacgaaa
    54001 accgtgtcgg tcaggtaact ggtctggcgt ggacggaagt gggcggtgac ttgctgacca
    54061 ttgaaaccgc gtgcgttccg ggtaaaggca aactgaccta taccggatcg cttggcgaag
    54121 tgatgcagga gtccattcag gcggcgttaa cggtggttcg tgcgcgtgcg gaaaaactgg
    54181 ggatcaaccc tgatttttat gaaaaacgcg acatccacgt ccacgtaccg gaaggtgcga
    54241 cgccgaaaga tggtccgagt gccggtattg ctatgtgcac cgcgctggtt tcttgcctga
    54301 ccggtaaccc ggttcgtgct gatgtggcaa tgaccggtga gatcactctg cgtggtcagg
    54361 tactgcctat cggtggtttg aaagaaaaac tactggcagc gcatcgcggc gggatcaaaa
    54421 cagtgttaat tccgttcgaa aataaacgcg atctggaaga gattcctgac aacgtaattg
    54481 ccgatctgga tattcatcct gtgaaacgca ttgaggaagt tctgactctg gcgctgcaaa
    54541 atgaaccgtc tggcatgcag gttgtgactg caaaatagtg acctcgcgca aaatgcacta
    54601 ataaaaacag ggctggcagg ctaattcgga cttgccagcc tttttttgtc tcgctaagtt
    54661 agatggcgga ttgggcttgc ccttattaag gggtgttgta aggggatggc tggcctgata
    54721 taactgctgc gcgttcgtac cttgaaggat tcaagtgcga tataaattat aaagaggaag
    54781 agaagagtga ataaatctca attgatcgac aagattgctg caggggctga tatctctaaa
    54841 gctgcggctg gccgtgcgtt agatgctatt attgcttccg taactgaatc tctgaaagaa
    54901 ggggatgatg tagcactggt aggttttggt acttttgccg ttaaagagcg tgctgcccgt
    54961 actggccgca atccgcagac cggtaaagag atcaccatcg ctgctgctaa agtaccgagc
    55021 ttccgtgcag gtaaagcact gaaagacgcg gtaaactaag cgttgtcccc agtggggatg
    55081 tgacgaagtt caagggcgca tctactgatg tgcctttttt atttgtattc ggtgactttc
    55141 tgcgtcttgt gggctgacaa ttgcccccgt ttcttgtcac aatatgcctt tgcgcgcatc
    55201 gatacgttgc gtgaggtaca cagtcatcta cagcggagtg ttgttacacc atgatggaca
    55261 gcttacgcac ggctgcaaac agtctcgtgc tcaagattat tttcggtatc attatcgtgt
    55321 cgttcatatt gaccggcgtg agtggttacc tgattggcgg aggcaataac tacgccgcaa
    55381 aagtgaatga ccaggaaatc agtcgtgggc aattcgagaa cgccttcaac agcgagcgta
    55441 atcgcatgca gcaacagctg ggcgatcaat actccgagct ggcagcgaac gaaggctata
    55501 tgaaaaccct gcgtcaacag gtgctgaatc gtctgatcga cgaggcgctg ctggatcagt
    55561 acgctcgtga gctgaaactg ggtatcagcg atgagcaggt taaacaggcg attttcgcga
    55621 ccccagcctt ccaggttgac ggcaaatttg ataacagccg ctataacggt atcctcaacc
    55681 agatggggat gaccgccgat cagtacgccc aggcgctgcg taaccagctc actacccaac
    55741 agctgattaa cggcgttgcc ggtaccgatt ttatgctgaa aggtgaaacc gacgagctgg
    55801 cggcactggt agttcaacaa cgcgtggtgc gtgaagcgac tatcgatgtt aacgcgctgg
    55861 cggcgaagca gcctgtgacc gaacaggaaa ttgccagcta ctacgaacaa aacaaaaaca
    55921 atttcatgac gccggaacaa ttccgcgtga gttacatcaa gctggatgcc gcaacgatgc
    55981 agcaaccggt tagcgatgcg gatatccaga gctactacga tcagcatcag gatcaattca
    56041 cccagccgca gcgtacccgt tacagcatca tccagaccaa aactgaagat gaagcgaaag
    56101 cggtacttga tgagctgaat aagggcggtg attttgctgc attagccaaa gaaaaatctg
    56161 ccgatattat ctctgcccgc aacggcggtg atatgggttg gttagaagat gccactatcc
    56221 cggacgaact gaaaaatgct ggtctgaaaa aaaaaggcca actgtctggt gttatcaaat
    56281 cttcggtcgg tttcctgatt gtgcgtctgg acgacattca gccagcgaaa gtgaaatcgt
    56341 tagacgaagt acgtgacgac attgcggcga aagtgaaaca cgaaaaagcc ctcgatgcgt
    56401 actacgcgct gcagcagaaa gtgagcgatg cggcaagcaa cgacaccgag tctctggccg
    56461 gtgcagagca agctgccggc gttaaagcca ctcagacggg ttggttcagc aaagataacc
    56521 tgccggaaga gttgaacttc aagccagttg ccgacgctat cttcaacggc ggtctggtcg
    56581 gtgaaaacgg cgcgccgggc atcaactctg acatcatcac cgtagacggc gaccgcgcat
    56641 tcgtgctgcg catcagcgag cacaaaccgg aagcggtgaa accgttggca gatgttcagg
    56701 aacaagttaa ggcactggtt cagcacaaca aagctgaaca acaggcgaaa gtggatgctg
    56761 agaaactgct ggttgatttg aaagcaggca aaggtgcgga agctatgcag gctgccggtc
    56821 tgaaatttgg cgagccgaaa accttaagtc gttccggtcg tgacccgatt agccaggctg
    56881 cgtttgcact gccgctgcca gcgaaagaca aaccgagcta cggtatggcg accgatatgc
    56941 aaggtaatgt agttctgctg gcgctggatg aagtgaagca aggttcaatg ccggaagatc
    57001 agaaaaaagc gatggtgcag ggtatcaccc agaacaacgc acaaatcgtc tttgaagctc
    57061 tgatgagtaa cctgcgtaaa gaggcgaaaa tcaaaattgg cgatgcgctg gaacagcaat
    57121 aatcctgaag ccgcctcgca aaaaaatgct ttgcagctgt aacaagaaaa ggtcgctttc
    57181 gcggcctttt ccatttctga acattgccat ttgtttactg ttttcactgc cgttaaggtg
    57241 attccactgt taacaaacaa ggagaaaaca gtatgaaaca cggaattaaa gcactgctca
    57301 ttaccctgtc cctggcctgt gccggaatgt ctcatagtgc tctggcggca gctcctgcgg
    57361 caaaaccgac aacggtagaa accaaagcgg aagctcctgc agcacaaagt aaagcagcag
    57421 ttccggcgaa agccagtgac gaagaaggca cccgggtcag cattaataat gccagcgcgg
    57481 aagagttagc ccgcgcgatg aatggcgttg gcctgaagaa agcgcaggcg attgtcagtt
    57541 atcgcgaaga gtacggtccg tttaaaacgg tggaggatct aaagcaggtg ccggggatgg
    57601 gcaattcgct ggtggagcgt aatctggcgg tattaaccct gtaattaatt tgcatagtgg
    57661 caattttttg ccagactgaa gaggtcatac cagttatgac ctctgtactt ataacaacaa
    57721 cgtaaggttc tcgcgctatg caaacacaaa tcaaagttcg tggatatcat ctcgatgttt
    57781 accagcacgt taataacgcc cgctatctgg agtttctcga agaagcccgc tgggatgggt
    57841 tggaaaatag cgacagtttt cagtggatga cggcccataa catcgctttc gtggtggtga
    57901 atatcaatat taactatcgt cgcccggcgg tattaagcga cttgttaacc attacgagcc
    57961 agttacagca attaaacggt aaaagcggca tcttaagcca ggtcattaca ctggagccgg
    58021 aagggcaggt ggtagcggat gcgcttatta cgtttgtttg tattgatctt aaaacgcaga
    58081 aagcattagc tctggaaggg gaattgcgcg aaaagctcga gcagatggtt aagtaaacgt
    58141 tttgtggtgc cggatgctca agccgcatcc ggcgacaccc ggaataatta cttcaacccg
    58201 gttttctgct tcatcgctgc catcaccgtc ggtttatcgg ccagataatg atgcaaaccg
    58261 ttggcacgta aattacacgc cgcacaatga ccgcaaccgt cgcctttaat gccgttataa
    58321 caggtcagcg tttcgttacg gactaaatcc agcttgccgt aataatctgc cagcgcccag
    58381 gtttccgctt tatcaatcca catcagcggc gtttcaaaac gaatgtcttt cgccataccc
    58441 agactgacgg catggttcag tgctttcaca aactcatcgc ggcaatccgg gtagccggag
    58501 aaatccgttt cgcagacgcc ggtaatcacc gcttctgctt ttacctgata cgcatatatt
    58561 gccgccagtg tcaggaacaa aatattacgc ccagggacaa acgtattcgg gatgccatcg
    58621 gcttcaggtt cataatctgg cactggaatg ctgtcacgtg tcaggctgct gaccgccagc
    58681 tcgttgagca gcgttacatc aagaacctta tgcgcgcgtg cccccagttt cagcgccagt
    58741 tcgcgtgcca cgtcgatttc cgcgcgatgt cgctgaccgt aatcgaacgt cacgcaatgg
    58801 acttcatcat attgttgtaa tgcctgcacc agacaggtgg tagaatcctg gcctccactg
    58861 aacacaacga cagcacgttt catagataat cctgcttgaa caataaaagc gttatggtaa
    58921 cgcctgcgat gaacccggac cagctattca ttgcgatggc gcgggaagcc aggcttcggt
    58981 aaattcaaac cagcctcgcg gtgtcagtcg cacaccgttc acgccgggag gggcgctaat
    59041 gcgatagtga tagttgaaca gcggcgtcag cgtcgcatct gccattaact ggctaaaaac
    59101 cgctttcagg gcattaaatc ggttttcctc atcaggcatt acttgcaccg cgtccagtgt
    59161 cgattgcaaa tgtgcatatg ctggagcgtc gaaaacatgt ggccatagtg gatcgcaacg
    59221 cagccattgc tccagggtat attccggtgc ttcgccaatt aatctgtcgc ccatcatgag
    59281 gtctgcgtgt gccagtaggg tcgtgtcgtc ccagtttttt gcgttatgaa aaataattgt
    59341 gagttcacag ccttccgctg ccagtgtcgc ctgtagacgt tctgccatgg tatgaagttc
    59401 tatcggtagg tgataaacca gcgtcaaggt tttcggtagt ttgacctcat ccggtacttg
    59461 ccattgcgga atagtccagc ctggcagtaa tgcatgactg gcggtgatca ggttttctcc
    59521 gacttctaac gtttgtaata aaccggattg atgaataatg gaaatcactt ttcgcgcctg
    59581 ccagagggac agtcggggac ttttgcgcaa cgttaaatag caaaaaccta aactgatgcc
    59641 actactgacc tggctgaccc gttgcagctc ctccggtttg ccgatggtga tttgcacggg
    59701 atgccgacaa ctggttccca aatctttttc gaaaagcggc ggagttatcc agtactcaac
    59761 cgctttaagc agcggatgac gtaaatggta ataatcatgg ctttccaggc gcaccagctc
    59821 cgctgtgaat tgtgttaagc gaaaaggacc cgtgccgatc agtgggaatt gcggatgcgc
    59881 cagatggctg caatagctcg ccagtcggtg cgcaagccag taatcggggc gatgtaaaaa
    59941 gaaggtcaga cactgcggat gggtgacttc aatacgcttc acgctaataa ataattgatc
    60001 ccgtgctggc agttgtaaca gcagcaataa tcgctggtgt aagtgtgagg cttttactgc
    60061 atcgccgtta tgccagtgta gggttgaacg aagataaaag tcccagcgta acccgtcagt
    60121 agaggtttcc cagtgatgtg ctaaatcgcc aatcgggcgc tgagtattat tatcgaagcg
    60181 ggtcaggccg gaaaatatct gcccggccag atgctgctcg gcacggccgg gcaaaaagcc
    60241 cggttgtagc ggttcgagcg ggcgatagta gggaatacgc aacgtgggcg tatcgttttg
    60301 ccattgtccg cccataaacg gctgtaacag agtgcgcagc tcacctgggg ccagttgcgc
    60361 cagctccagc acatcttgct gctttccggt ttccagcgcc tgttccatca tcgcattgcg
    60421 tagcgattct ggcgtgacca gaaagcgtag ttgcccgcgt tttccgcgtc ctgactgcgc
    60481 ctgccactcc agccatcccg cctcctgtgc ctggcgtaac agcgtacgaa catggcgttc
    60541 gctgcaaaaa cagcgttcgg ccagttcgct gacggtgacg gtttgcggct ttccggcgga
    60601 aggttgccac agacgttgat actggttaag acggttgagc agtcgcatat aaacccggaa
    60661 caatattatt taactattca ctattacttc cgtatatatc aggtgatact caatcaccat
    60721 taaccgtgtc acagagtgga gaaagaaatg gctcgtctgg cagcatttga tatggatggc
    60781 actttattga tgcctgacca tcatttaggt gagaaaaccc tctctacttt ggcgcgactg
    60841 cgtgaacgcg acattaccct cacttttgcc actgggcgtc atgcgctgga gatgcagcat
    60901 attctcgggg cgctatcgct ggatgcatat ttgattaccg gcaacggaac gcgcgtgcat
    60961 tctctggaag gtgaactttt acatcgtgat gatttacctg cggatgtcgc ggagctggtg
    61021 ctgtatcagc agtgggatac ccgagccagc atgcatatct tcaatgacga cggttggttt
    61081 accgggaaag agatccctgc gttgttgcag gcatttgtct atagtggttt tcgttatcag
    61141 ataatcgatg tcaaaaaaat gccactcggc agcgtcacca agatctgctt ctgtggcgat
    61201 cacgacgatc ttacacgctt gcagatccag ctatacgaag cattaggcga gcgtgcacat
    61261 ttgtgttttt ccgccacgga ttgcctcgaa gtgctgccgg tgggctgcaa taaaggcgct
    61321 gcattgacgg tgctgaccca acatttaggt ttatcgttgc gcgattgcat ggcctttggt
    61381 gatgcgatga acgatcgcga aatgttaggc aacgtcggta gcggatttat tatgggcaat
    61441 gcgatgccgc aattgcgcgc ggagctcccg catttaccgg tgattggaca ttgccgaaat
    61501 caggctgtct ctcactattt gacgcactgg ctggactatc cacatctacc ttattccccc
    61561 gaataacgag atcccttcca gcaccgggca attgcccggt tttttttgcg ttgaatttgt
    61621 cattttgtgc cgtggtgttt aaaccgcaca gaataaattg tcgcgatttc acctttaaaa
    61681 tagaattaaa agagaaaaaa attctctgtg gaagggctat gttagataaa attgaccgta
    61741 agctgctggc cttactacag caggattgta ccctctcttt gcaggcactg gctgaagccg
    61801 ttaatctgac aaccaccccg tgctggaagc gtctgaaacg gctggaggac gacggtattc
    61861 ttatcggcaa agtcgccctg ctggatccgg aaaaaatagg cctcggcctg accgcctttg
    61921 tgctgataaa aacgcaacat cacagcagcg aatggtattg ccgctttgtc acggtggtta
    61981 ccgaaatgcc agaagtgctg gggttctggc gcatggccgg tgaatacgat tatctgatgc
    62041 gcgtccaggt tgccgacatg aaacgctacg acgagtttta taagcgtttg gtaaacagcg
    62101 tgccggggct gtcggacgtc acttccagct tcgcgatgga acagattaaa tacaccactt
    62161 ctttacccat cgaataaata tccagaatca ggtcaggaca caacgcgtgc gattatttgc
    62221 tcaattaagc tggtatttcc gtcgggaatg gcgtcgctat ctcggggctg tcgccttgct
    62281 tgtcattatc gcgatgctgc aactggttcc gccaaaagtg gttggtattg ttgttgatgg
    62341 cgtgacagaa caacacttta ctaccgggca gatcctgatg tggatcgcca ccatggtgct
    62401 gattgccgtt gtggtttatc tcctgcgtta cgtctggcgt gtattgctgt ttggcgcgtc
    62461 ttatcaactg gctgttgaac tgcgtgaaga ttattaccgt cagctaagcc ggcagcatcc
    62521 tgagttttac ctgcgtcatc gcacgggtga cctcatggct cgtgcgacca atgacgtcga
    62581 tcgcgtcgtg tttgccgccg gagaaggggt gctgacgctg gtggattcac tggtaatggg
    62641 ctgcgctgtg ttgattatga tgtctacgca aattagctgg cagttgacct tatttgccct
    62701 gttgccgatg ccagtgatgg cgatcatgat taagcgcaac ggcgatgcct tgcatgaacg
    62761 ctttaagctg gcgcaggcgg cgttttccag tcttaatgac cgcacccagg aaagcctcac
    62821 cagtatccgc atgatcaaag cttttggtct ggaagatcgc cagtcggcgt tatttgccga
    62881 ggatgccgaa gataccggca aaaaaaacat gcgggtggcg cgtattgatg ctcgtttcga
    62941 cccgaccatc tatatcgcga ttggtatggc gaacttgctg gcgattggcg gcggtagttg
    63001 gatggtggtg cagggcagtt taacgctggg ccagctcacc agttttatga tgtatttagg
    63061 tctgatgatt tggccaatgc tggcgctggc atggatgttt aacatagtgg aacgtggtag
    63121 tgctgcgtac agccgcattc gcgcgatgct ggcggaagcg ccggtggtga atgatggtag
    63181 cgaacccgtg ccggaaggtc gtggcgaact ggatgtaaat attcgccagt tcacgtatcc
    63241 gcagactgac catcctgcgc tggaaaacgt caatttcgcc ctgaaacccg gtcagatgct
    63301 gggtatttgc gggccgactg gttccggcaa aagtaccctg ttgtcgctca ttcagcgtca
    63361 tttcgacgtc agcgaggggg atatccgctt tcatgatatt cctctgacga agttacaact
    63421 cgatagctgg cgtagccgcc tggcggtagt tagccagaca ccgttccttt tttccgacac
    63481 cgtagcgaac aacatcgcgt tgggttgccc gaatgccacc cagcaagaga ttgagcatgt
    63541 cgcgcggtta gccagcgtgc atgacgatat tttgcgttta ccgcaaggtt acgatacaga
    63601 agtgggcgag cgcggcgtga tgctttccgg tgggcaaaaa cagcgtatct ccattgctcg
    63661 tgcgttatta gtcaacgcgg aaatcctcat ccttgatgat gcgctttcgg cagtagacgg
    63721 acgcacagag caccagatcc tgcataacct gcgtcagtgg gggcagggaa gaacggtaat
    63781 catcagcgcc catcgccttt ctgcactgac ggaagccagt gaaattattg tgatgcagca
    63841 cggacatatc gctcagcgtg gcaatcatga tgagctggcg caacaaagcg gctggtatcg
    63901 cgatatgtat cgctatcaac aactggaggc ggcgctcgac gacgctccgg aaattcgcga
    63961 ggaggccatc gatgcgtagt tttagccaac tgtggccgac tctcaagcgt ctgttagcgt
    64021 acggttcgcc gtggcgtaaa ccgctgggga ttgcggtcat aatgatgtgg gttgcggcag
    64081 cggcagaagt cagtgggccg ctgcttatca gctattttat cgacaatatg gtagcgaaaa
    64141 ataacctgcc gttgaaagtg gttgcagggc tggctgcggc gtatgttggg ctgcaactgt
    64201 ttgccgccgg gctacattac gcgcagtcgc tgctgtttaa tcgggcggca gtaggcgtag
    64261 tgcaacagtt gcgtaccgac gtgatggatg ctgcgttacg ccagccatta agcgagtttg
    64321 atacccaacc cgtcgggcag gtgatttccc gcgtcactaa tgacactgaa gtgatccgcg
    64381 acctctacgt taccgtagtg gcaactgtcc tgcgtagcgc cgcactggtg ggcgcgatgc
    64441 tggtggcgat gttcagcctc gactggcgaa tggcgctggt ggcgataatg attttcccgg
    64501 tggtgctggt ggtaatggtg atatatcagc gttacagcac gccgattgtc cgtcgtgtgc
    64561 gcgcttatct ggcagatatc aacgacggct ttaacgaaat tatcaatggc atgagcgtta
    64621 tccagcagtt ccgtcagcag gcgcgatttg gcgaacgtat gggtgaggcc agtcgttcac
    64681 actatatggc gaggatgcaa accctgcgcc tcgacggttt tctgctgcgt ccgctgctga
    64741 gtctgttttc atcgctcatt ctttgtggct tgttgatgct gtttggcttc tccgccagcg
    64801 gcaccattga agtgggcgtg ttgtatgcgt tcatcagtta tttggggcga cttaacgaac
    64861 ccttaatcga actgaccacg cagcaggcga tgctgcaaca ggctgttgtt gctggtgagc
    64921 gcgtttttga gctgatggac ggaccgcgcc agcaatatgg caatgatgat cgcccgttgc
    64981 agagcggcac catcgacgtc gataacgtgt catttgctta tcgcgatgac aatctggtgc
    65041 taaagaacat taatctctct gtgccttcgc gcaactttgt ggcgctggtc gggcataccg
    65101 gcagtggcaa aagcaccctc gccagtttat tgatgggcta ttacccgcta acggaaggtg
    65161 agattcgcct tgatggtcgt ccattaagta cgctaagtca cagcgcgctg cgccagggcg
    65221 tggcaatggt gcagcaagat ccggtggtgc tggcggatac cttcctcgcc aacgtgacgc
    65281 tgggacgtga tatctctgaa gaacgcgtct ggcaggcgct ggaaatcgtg cagctggcgg
    65341 agctggcgcg tagcatgagt gatggtattt acacgccgct tggcgagcag gggaataatc
    65401 tctcagtcgg gcaaaagcaa ctgctggcac tggcgcgcgt gctggtggag acgccgcaaa
    65461 tcctgatcct tgatgaggca accgccagca ttgactccgg gactgaacag gcgattcaac
    65521 atgctctggc ggcggtgcgt gaacatacta cgcttgtggt gattgctcac cgcttatcga
    65581 ctattgttga tgccaacacc attctggtgc ttcatcgtgg gcaagccgtg gagcagggca
    65641 ctcaccagca actgctggcg gcccagggac gatactggca gatgtatcaa ctgcaacttg
    65701 cgggcgaaga gctggcagcc agcgtgcgtg aagaggaatc attgagcgcc tgaatagcgc
    65761 aatatttcat cgttggtgca aaaatgtaac gcactgtgca ctgtcatagt gcgttttcat
    65821 tttcaatctt cttaacttcc tgctctcttt ctcgtttttc atttttggca caccgcttgc
    65881 aataccttct tcgtgtagca gaaccattac cgaattctga ccggagggga tctatgaagc
    65941 tggtgaccgt gattatcaaa ccattcaaac ttgaagacgt tcgtgaagcg ttatcttcca
    66001 ttggtattca gggcctgacc gtcaccgaag tgaaaggttt cgggcgtcag aaaggacatg
    66061 ccgagctgta ccggggggcg gaatacagcg tcaatttcct gccaaaagta aaaattgatg
    66121 tggcgattgc tgatgaccaa ctcgatgaag tgatcgatat cgtcagtaag gcggcttaca
    66181 ccggaaaaat tggcgacggc aaaatcttcg tcgctgaatt gcaacgcgtc attcgtattc
    66241 gtaccggcga agccgacgaa gcggcgctgt aatctctggc acacagcaac aggaacgaaa
    66301 aatgaagata gcgacgataa aaactgggct tgcttcactg gcgatgctgc cgggactggt
    66361 aatggctgca cctgcggtgg ccgataaagc cgacaatgcg tttatgatga tttgtactgc
    66421 gctggtgctg tttatgacta ttccggggat tgccctgttt tacggtgggt tgattcgcgg
    66481 caaaaacgtg ctgtcgatgc tgacgcaggt gacggtgacg tttgcactgg tctgtattct
    66541 ctgggtggtt tacggttact cgctggcgtt tggtgaggga aacaacttct tcggcaacat
    66601 taacgggttg atgctgaaaa acatcgaact gacggcggtg atgggcagca tttatcagta
    66661 tatccacgta gcgtttcagg gatcgtttgc ctgcattacc gtaggcttga tagttggagc
    66721 actggcggaa cgaatccgct tctcagctgt gttgattttc gtggtggtat ggctgacgct
    66781 ctcctacatt ccgattgcgc atatggtctg gggcggcggt ttgctggctt ctcacggtgc
    66841 actggatttc gcgggtggca cggtggtgca cattaacgcc gcaatcgccg gtctggtggg
    66901 cgcgtatctg ataggcaaac gcgtgggctt cggcaaagag gcgtttaaac cgcacaacct
    66961 gccgatggtc ttcaccggga ctgccattct ctatatcggt tggtttggct ttaacgccgg
    67021 gtcagcgggc acggcgaatg aaatcgcagc actggcattt gtgaatactg tggtcgcaac
    67081 ggcggcggca attcttggct ggatcttcgg tgaatgggcg ctgcgtggta agccttcact
    67141 gctgggagcg tgttcaggcg cgattgccgg tctggtcggc gtgacgccag cgtgtggcta
    67201 cattggtgtt ggcggcgcgt tgattatcgg cgtggtagct ggtctggcgg gcttgtgggg
    67261 cgttaccatg ctcaaacgct tgttgcgggt ggatgatccc tgcgatgtct tcggtgtgca
    67321 cggcgtttgt ggcattgtcg gctgtatcat gaccgggatt tttgctgcca gctcgctggg
    67381 cggcgtgggc ttcgctgaag gtgtgacgat gggccatcag ttgctggtac agctggaaag
    67441 catcgccatt acgatcgtct ggtccggtgt ggtggcattt attggctaca aactggcgga
    67501 tctgacggtt ggtctgcgtg tgccggaaga acaagagcga gaagggctgg acgtcaacag
    67561 ccacggcgag aatgcctata acgcgtaaca agcactgcaa aaaacagccg gacggttttc
    67621 acctccggct atttttttaa ttatgattac gcattacccc ttcctgaacg gtcgaggcaa
    67681 ccagcacgcc gtcctgagta taaaactcac cgcgcacaaa gccgcgtgcg ctggacgccg
    67741 aggtgctctc cacgctatac agcagccatt cattcaaatt aaacgggcga tggaaccaca
    67801 tggaatggtc gatggtcgct atctgaattc ccggttcgag aaaaccgatg ccgtgcggct
    67861 gtagagctac cggcaggaag ttaagatcag aagcgtaacc gagcagatac tgatgaacgc
    67921 gcagatcatc cggcacgcta ccatttgcgc ggatccacac ctgacgatgc ggttctgcga
    67981 cgtgaccttt caatgggtta tgaaattcca ccggacggac ttccagcgga cgatcgcaga
    68041 tgaatttatc tttcagcact ggcggcagca ggtgcgccag cgattgggcg atttgtgttt
    68101 ccgaaggcag gccatcaggc gctggcgcgg acggcattgt tttttgatgt tcgaaacccg
    68161 cttctggtgc ctggaaagag gccgtcatat aaaaaatcgg tttgccgttt tgaatagcag
    68221 caacccggcg ggcgctgaag ctgttaccgt cacgcagcgt ttcgacatca taaataatcg
    68281 gcttcttact atcgcctggg cgaagaaagt agctgtgaaa cgaatgtacc agccgctctt
    68341 cagggacggt ctcttttgca gcatacaagg cctgacccac gacctggccg ccaaacacct
    68401 ggcgtaaacc taaatcttca ctctggccgc gaaagagtcc ttcctcaatt ttttccagat
    68461 ttaacaatgt cagtaaattt tttagcgcct gactcatata actctccagt aacaaagctg
    68521 ccgcagcaag ccaaagtgag ttgagtataa cgcaaatttg ccactggtcc gatgggtgca
    68581 atggtctgaa ttacgggcta attacaggca gaaatgcgtg atgtgtgcca cacttgttga
    68641 cgttactatt ttgttaacca ctcttccggc gaggaaagtt agcccgctgg tgcattgata
    68701 ataaggagaa gtgaatgaaa ctcgtgcaca tggccagtgg tttagcggtt gcgattgcgt
    68761 tggcggcttg cgcagataaa agcgcggata ttcagacgcc agcaccggct gcaaatacgt
    68821 ctatttcagc aacacaacct gctatccagc aaccgaatgt ctccggtacc gtctggatcc
    68881 gtcagaaagt cgcactgccg cctgatgctg tgctgaccgt gacactttct gacgcgtcgt
    68941 tagccgatgc gccgtcaaaa gtgctggcgc agaaagcggt gcgtaccgaa ggtaaacagt
    69001 caccattcag ctttgttctg ccatttaacc cggcagatgt tcagccgaac gcgcgtattc
    69061 tattgagtgc ggcgattacc gtgaatgaca aactggtatt tattaccgat accgttcagc
    69121 cggtgatcaa tcagggcgga actaaagccg acctgacatt ggtgccggtg cagcaaaccg
    69181 ccgtgccggt tcaggccagc ggtggcgcaa cggctaccgt accttcgact tcaccaactc
    69241 aggtgaatcc gtcttcggca gttccagctc ctacgcaata ttaagcgcag ttaaccctct
    69301 ccgcccggag agggttagta gttccagcga taacgctgca agtcgatttg cccgcttccc
    69361 gataccatca caccttctgc cagtaatgcc tgtcgttgac gctgtaaatc cggtccggtt
    69421 agcgaaattg tgccgtggcg attaaccacc cggtgccagg gcaaggtgct gccttcgggg
    69481 agacgcttta acacaccgcc tacctggcgc gcggcgcgcg gcgatcccgc cagtttcgcc
    69541 acatcaccgt aagtggtgac atagccttcg ggaatagcgg cgacgatttg ccagacgcgt
    69601 tggggaaatg aatcttcttt ttccatcttt tcttcctgag gtaatttttc agcataatct
    69661 ggaaaaacgc ccgagtgaag ccgcattgcg caagaaacca gcatctggca cgcaatgggt
    69721 tgcaattagc cggggcagca gtgataatgc gcctgcgcgt tggttctcaa cgctctcaat
    69781 gggggctctg ttggttctcc cgcaacgcta ctctgtttac caggtcaggt ccggaaggaa
    69841 gcagccaagg cagatgacgc gtgtgccggg atgtagctgg cagggccccc acccatttct
    69901 gcctcccccc gtttcgtcaa aaaatcccaa catggctaaa ctttaaccac aactgacgtc
    69961 gcaagaattg tctggctgcg cagtacgctt cggaggtatg tctgatgaag tatgttgatg
    70021 gttttgttgt tgccgttcct gccgataaaa aggatgccta tcgggaaatg gccgctaagg
    70081 ccgcgccatt atttaaagag ttcggtgcgc ttcgtattgt cgaatgctgg gccagcgatg
    70141 taccggatgg taaagtgacc gattttcgta tggcggtgaa agcggaagag aatgaagagg
    70201 ttgtctttag ctggattgaa tacccttcaa aagaggtccg cgacgctgct aatcaaaaga
    70261 tgatgtcgga cccacggatg aaagagttcg gcgagtccat gccgtttgac ggcaagcgaa
    70321 tgatctatgg tggattcgaa tcaatcatcg acgagtaggt agcgtgacgg gctgtgcggc
    70381 gtgccgtcag ccctaaaatt cacaaatgtt gctcggccca gcgtaagaaa tcctctttcg
    70441 gtaatgcctt gctgtagagc cagccctgac cgtagtgcac gccatgctgg cgtaaccact
    70501 cttcttgttt actggtttcg attccctccg ctaccatttt cagtttcagt gtttttgcca
    70561 tttcgatgat atgcggcgtg acatttttat attccagcgc atcaacgaaa gatttatcga
    70621 tcttcagaat gtcgacatcc agatcctgta aataacttaa acttgaatac cccgtaccaa
    70681 aatcatcgag ataaatttca tggcccgcct cccggtagcg agaaattatc ggggcgctgg
    70741 ttttcggatc ggcaaactcg cgttcagtga gttcaagcgc gatctgtctg ggattaacct
    70801 gatagtgatt gatcatttca cgcagcaatt gcgggatttt ttccgaggtg agcacggtgg
    70861 attcaagatt gatcgaaata tgctgctgcg gatgctgacg cagccagtcg cccatatctt
    70921 cgaagacgct tcttatgatc agtagcgtca acggctcaga aaggcccgtt tgctgtgcca
    70981 gcggaataaa actatctggt gacaaccaac taccgtctgt ctgcggccag cgcgccagtg
    71041 cctcagcacc gacaattttg ccattggcta aggagacaat cggctgatag tgcacgcaaa
    71101 tatcacgatt ttcgatagca tcctgcagcc gatgatgcgg tgactggata cggcgcaaaa
    71161 tacgcagcac aaacatcgct gccagcaggc caatcaccaa cccgagcggt aaccaaataa
    71221 agacttgccg atgccagcct ttctgcaaca ttttcgttga agaccaggtg atgatcgaaa
    71281 tattcatttc cgggaagggc aggatatcgt aaatgattcc attattttcg atatgctcac
    71341 cgggtgtttt ttgtagtctg gtaataattc cctgagcaat ttcatcgctg ctggttatca
    71401 caacgttatg ggcattgcca ataatggcgg catcaatttg ccatgagcta tagggaatga
    71461 catcaataaa ggaagcgggg tcgatcatga cgacataatg tgccgttccc atggcaacca
    71521 tgtaacggat aatgcctaaa tcgttatgcg atgttaacca gacacgataa ccatctttcg
    71581 aaattttacc tggctcgggg aaggtatcgg gcgggctttc atgctccaga gacgaacact
    71641 ggggaacgtt gttatcgata taaaccactt cctgaatata gcgataactg taagataccc
    71701 gacgcatttc catgagatgg gcttcgctac aggcagcgcc ttgccatctt tccagctctt
    71761 gcagcgcatc tttcccttgt gtcgccacct tattggcccg aatagcgacg cgggaggaat
    71821 acgtattcag ctcttcaata aatgatgttt ctacctgctg atgggccagc cagatgctta
    71881 agccgacagg cagcaatact gaaagaatca gtactcccga aataaggccg accagatgtc
    71941 gtgttctcac cagcatatcc ttattaacac agtgtaggga aagtatatct gattgtggaa
    72001 aaaggctggc aggagataag gaggtagagc cgggatttca tgaagggatg ggtggaaacg
    72061 aacgttgtca tttgaccagg cgcagagctt tacgcctggt atgtaaccct aacttaagat
    72121 gttgattcgg cgcgagcgag ggtaaaaata tcgtaaaaag atagttcacc tttacggacg
    72181 atcatttttt gcagttgttc tttatgaaca tccgcgacac gcggagagtt caggatggct
    72241 tcaactagcg cggcaggtac aaagcgcgtg ttgtaccatt cgccgttata gcaaacgcgg
    72301 aaatccagca cgtcgatatc ttcatagcgg tagcgtgggt agacatcgaa aaagaagaaa
    72361 ccattaagca ggataaataa tacaaccagt agccagacag agcctgaaag cgtttcggac
    72421 tgcaacatca ctgccagggt ggcaaaaaaa gcaacgtaca taccgataaa caaccccgga
    72481 tgtttacgga taaaactgat actaaagcgg ggtttgttgt cgcgtttttc gcgggtattt
    72541 agagactcaa ttgtttcggt cagcaggcgt tgtatttcgg tcatcgtttg ccttcgtggt
    72601 atctgcaact ttccagggaa caccctattt taacgtggga atagtagagg gaaagtaaca
    72661 gatttaccac aattttgcat aacagttgcg aaaaaccgat tacaattttt taattattct
    72721 ggctggatta ccgcccacga caacgttgtc cgggacatct tttgtgacaa ctgcacctga
    72781 ggctaccacg acgttatcac caatggtcac accagggtta atgaccgcac gtccgccaat
    72841 ccagacgtta ttaccgatgg tgacgggttt ccccagttca gcaccgctat tacgtgctac
    72901 agggtcgatg ggatgtgttg ccgtgtagat atgaacgcct ggtgccaaca tacagttatc
    72961 accaatgcga atagggcaga catcaagcat cacgcaatcg aagttggcga aaaaattatt
    73021 gccgagaaaa atgttataac catagtcaca gcgaaacgtt ggctcaatat aagcctctgt
    73081 cacctgaccg aataaatcag caagaatttg ctggcgtaat gtgtgctctt ccgccaggga
    73141 atgattgtat cggtgaataa gccgacgagc gcgcaggcga tcgcgagata acgcctcatc
    73201 tgccgagtga tacaactcac cagcaatcat cttttctttt tctgtgctca ttactgacct
    73261 ccggttgctg aaaatgcggc aacacagtaa gacgaaaagt cagtgaagag aacgttccca
    73321 ttacatctgt gtgatgaaga tcacataggg tatgccttca tggcgaaaga attataaaac
    73381 tcttaataaa cagccggtta tagctccgaa agcgagttat ttattagcga ataaatttcc
    73441 atactgagga agggatcttg tcgtacagtt tattcatggt caattcggcg aggcggtgat
    73501 ctgcggctga gtaaaatacc gccagctcat tatctgataa ttcgtattta tttttctcga
    73561 taacacgctc cagcgtgtca attgtctggc aacgacgtaa acgcattaaa taatcggttt
    73621 ttgttaaagg tttttcggac atacttctac ccatggttgt aataattcta acaagataaa
    73681 ctcgcaggat tctctttcgt cgcattgaca aaacaacgga atagtcgatt acctgacttc
    73741 cgccatttct gaagatcctg catattaata ccataactac tgaacaacat aaaggtgtca
    73801 tccagatatt cgtcgatctg ctcaatgagc ttattgtctt cattatactt aattttgtaa
    73861 ttaagtgcga aggtcgcaat atgctcaatc agttcattca actggaggtt gatcgccgag
    73921 gttgggtcgt taacccagcc atgattgctt tcttcaaggt ttgcaagaca gtcatgatac
    73981 agggtttcac agagaaactt aagctgcgcg atatcatgtc ttttgggtga gtattcatcc
    74041 ataacgcgtc cccttcttag cggttgaact aacggacacc tttcgggatg gaaaaaactt
    74101 actgacctgg acttgccctc tcgtttgtta gcttaactat aagccactct ttgcaggttt
    74161 tcatcgcatt attacgaaaa attacaatta ggaaggaagc gggacaggac tcactttttc
    74221 atactagcac taacccgcag caggtaaaag cagtttgctc gaactgttca taacatttca
    74281 ggattatcgc cagatgaaat tggtaacact aataccagga ttgctctgaa tatgacgtaa
    74341 taaccgagga atgaa
//
LOCUS       DAZFLD010000002        65998 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.002, whole genome shotgun sequence.
ACCESSION   DAZFLD010000002 DAZFLD010000000
VERSION     DAZFLD010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 65998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65998
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.002"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(56..535)
                     /gene="folK"
                     /locus_tag="ACHKBL_000075"
     CDS             complement(56..535)
                     /gene="folK"
                     /locus_tag="ACHKBL_000075"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HFO5817005.1"
                     /translation="MTVAYIAIGSNLASPLEQVNAALKALGDIPESRILAVSSFYRTP
                     PLGPQDQPDYLNAAVALKTTLAPEELLNHTQRIELQQGRVRKAERWGPRTLDLDIMLF
                     GNEVINTERLTVPHYDMKNRGFMLWPLFEIAPELLFPDGLSLVEALQAKGFNELDKW"
     gene            complement(532..1929)
                     /gene="pcnB"
                     /locus_tag="ACHKBL_000076"
     CDS             complement(532..1929)
                     /gene="pcnB"
                     /locus_tag="ACHKBL_000076"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414685.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:1990817 - poly(A) RNA polymerase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HFO5817006.1"
                     /translation="MFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQHAISRKDI
                     SENALKVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKLFRNCRLV
                     GRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGSIEEDAQR
                     RDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLRAVRFAAK
                     LGMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYETYKLLCEYHLFQPLF
                     PTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLLETAQKIA
                     QESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQGKRAWKLL
                     EHPKFRAAYDLLALRAEVERNAELQRLVKWWGEFQVSAPPDQKGMLNELDEEPSPRRR
                     TRRPRKRAPRREGTA"
     gene            complement(1989..2885)
                     /gene="gluQRS"
                     /locus_tag="ACHKBL_000077"
     CDS             complement(1989..2885)
                     /gene="gluQRS"
                     /locus_tag="ACHKBL_000077"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001432381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HFO5817007.1"
                     /translation="MTDTHYIGRFAPSPSGELHFGSLIAALGSYLQARARQGRWLVRI
                     EDIDPPREVPGAAETILRQLEHYGLHWDGDVLWQSQRHDAYREALAWLHEQGLSYYCT
                     CTRARIQSIGGIYDGHCRELHHGPDNAAVRIRQQHPVTLFTDQLRGIIHANEKLARED
                     FIIHRRDGLFAYNLAVVVDDHFQGVSEIVRGADLIEPTVRQISLYQLFGWKVPDYIHL
                     PLALNTQGAKLSKQNHAPALPKGDPRPVLIAALQFLGQQAEAHWQDFSVEQILQSAVK
                     NWRLTAVPESAIVNSTFSNASC"
     gene            complement(2952..3407)
                     /gene="dksA"
                     /locus_tag="ACHKBL_000078"
     CDS             complement(2952..3407)
                     /gene="dksA"
                     /locus_tag="ACHKBL_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002209354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HFO5817008.1"
                     /translation="MQEGQNRKTSSLSILAIAGVEPYQEKPGEEYMNEAQLAHFRRIL
                     EAWRNQLRDEVDRTVTHMQDEAANFPDPVDRAAQEEEFSLELRNRDRERKLIKKIEKT
                     LKKVEDEDFGYCESCGVEIGIRRLEARPTADLCIDCKTLAEIREKQMAG"
     gene            complement(3585..4289)
                     /gene="sfsA"
                     /locus_tag="ACHKBL_000079"
     CDS             complement(3585..4289)
                     /gene="sfsA"
                     /locus_tag="ACHKBL_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HFO5817009.1"
                     /translation="MEFSPPLQRATLIQRYKRFLADVITPDGRELTLHCPNTGAMTSC
                     ATPGDTVWYSTSDNTKRKYPHTWELTQSQSGAFICVNTLWANRLTKEAILNESISELS
                     GYSSLKSEVKYGAERSRIDFMLQADSRPDCYIEVKSVTLAENEQGYFPDAVTERGQKH
                     LRELMSVAAEGQRAVIFFAVLHSAITRFSPARHIDEKYAQLLSEAQQRGVEILAYKAE
                     LSAEGMALKKSLPVTL"
     gene            complement(4304..4834)
                     /gene="thpR"
                     /locus_tag="ACHKBL_000080"
     CDS             complement(4304..4834)
                     /gene="thpR"
                     /locus_tag="ACHKBL_000080"
                     /EC_number="3.1.4.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004113 - 2',3'-cyclic-nucleotide
                     3'-phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0008664 - RNA 2',3'-cyclic
                     3'-phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HFO5817010.1"
                     /translation="MSEPQRLFFAIDLPAEIREQIIHWRATHFPPDAGRPVAADNLHL
                     TLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQL
                     ANMLRSQAARSGCFQSNRPFHPHITLLRDASEAVTIPPPGFNWSYTVTEFTLYASSFA
                     RGRTRYTPLKRWALTQ"
     gene            4908..7337
                     /gene="hrpB"
                     /locus_tag="ACHKBL_000081"
     CDS             4908..7337
                     /gene="hrpB"
                     /locus_tag="ACHKBL_000081"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HFO5817011.1"
                     /translation="MSSLPVAAVLPELLAALDCASQVLLSAPTGAGKSTWLPLQLLAH
                     PGINGKIILLEPRRLAARNVAQRLAELLNEKPGDTVGYRMRAQNCVGPNTRLEVVTEG
                     VLTRMIQRDPELSGVGLVILDEFHERSLQADLALALLLDVQQGLRDDLKLLIMSATLD
                     NDRLQQMLPEAPVVISEGRSFPVERRYLPLPAHQRFDEAVAVATAEMLRQESGSLLLF
                     LPGVGEIQRVQEQLASRIGSDVLLCPLYGALSLNDQRKAILPAPQGMRKVVLATNIAE
                     TSLTIEGIRLVVDCAQERVAHFDPRTGLTRLVTQRVSQASMTQRAGRAGRLEPGICLH
                     LIAKEQAERAAAQSEPEILQSDLSGLLMELLQWGCSDTAQMSWLDQPPTVNLQAAKRL
                     LQMLGALEGERLSAQGQKMAVLGSDPRLAAMLVSAKNDDEAATAAKIAAILEEPPRMG
                     NSDLGVAFSRNQPAWQQRSQQLLKRLNVRGGEADSSLIAPLLAGAYADRIACRRGQDG
                     RYQLANGMGAMLDADDALSRYEWLIAPLLLQGSASPDARILLALPVDIDELVQRCPQL
                     VQQSDTVEWDDAQGTLKAWRRLQIGQLTVKVQPLAKPSEDELHQAMLNGIRDKGLSVL
                     NWTAEAEQLRLRLLFAAKWLPEYDWPAVDDESLLATLETWLLPHMSGVHSLRGLKSLD
                     IYQALRGLLDWGMQQRLDSELPAHYTVPTGSRIAIRYHEDKPPALAVRMQEMFGEATN
                     PTIAQGRVPLVLELLSPAQRPLQITRDLSAFWKGAYREVQKEMKGRYPKHVWPDDPAN
                     TAPTRRTKKYS"
     gene            7431..9962
                     /gene="mrcB"
                     /locus_tag="ACHKBL_000082"
     CDS             7431..9962
                     /gene="mrcB"
                     /locus_tag="ACHKBL_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyl
                     transferase/transpeptidase"
                     /protein_id="HFO5817012.1"
                     /translation="MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDYDDYDDYEDEEP
                     MPRKGKGKGKGRKPRGKRGWLWLLLKLAIVFAVLIAIYGVYLDQKIRSRIDGKVWQLP
                     AAVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFDF
                     PDSKEGQVRARLTFDGDHLATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPRS
                     GFPDLLVDTLLATEDRHFYEHDGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNLF
                     LSSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGDNEIRGFPLASLYYF
                     GRPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDQELYDM
                     LSARPLGVQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAAE
                     KAAVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRSIG
                     SLAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGQVWSPQNDDRRYSESGRVMLVD
                     ALTRSMNVPTVNLGMALGLPAVTETWIKLGVPKDQLNPVPAMLLGALNLTPIEVAQAF
                     QTIASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGTG
                     RQLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTITWVGRDNNQPTKLYGASGAMS
                     IYQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQQSEM
                     QQQPSGNPFDQSSQPQQQPQQQPAQQEQKDSDGVAGWIKDMFGSN"
     gene            10182..12371
                     /gene="fhuA"
                     /locus_tag="ACHKBL_000083"
     CDS             10182..12371
                     /gene="fhuA"
                     /locus_tag="ACHKBL_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0038023 - signaling receptor activity
                     [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrichrome porin FhuA"
                     /protein_id="HFO5817013.1"
                     /translation="MARSKTAQPKHSLRKIAVVVATAVSGMSVYAQAAVEPKEDTITV
                     TAAPAPQESAWGPAATIAAKHSATATKTDTPIEKTPQSISVVTNEEMQMHQFQSVKEA
                     LGYTPGVTVSSRGASNTYDFVIIRGFSSVGLNQNNYLDGLKLQGNFYNDAVIDPYMLE
                     RVELMRGPTSVLYGKSNPGGIISMVSKRPTTEPLKEIQFKMGTDNLFQTGFDFSDALD
                     DNGEFSYRLTGLARSTNEQQKNSESQRYTIAPSFSWRPDDKTNFTFLSYFQNEPETGY
                     YGWLPKEGTVEPLPNGKRLPTDFNEGASNNTYSRNQKMVGYSFEHGFNDTFTVRQNLR
                     FSEMKTSQKSVYGTGIASDGHTLNRGTVVDNERLQNFSVDTQLESKFATGEVEHTLLT
                     GVDFMRMRNDINASFGSAPSIDLYNKYHPEYFAFGNAEPYQMNESKQTGIYVQDQAEW
                     NKWVFTLGGRYDWSKQATTVRENSYTPTEGYIERNDHQFTWRGGVNYLFDNGISPYFS
                     YSQSFEPSAFDLWSNPRVSYKPSKGEQYEAGVKYVPNDMPVVVTGAVYQLTKTNNLTA
                     DPTNPLAQVPAGEIRARGVELEAKAALNANINLTASYTYTDAEYTKDTNLKGKTPEQV
                     PEHMASLWGDYTFNEGPLSGLTLGTGGRFIGSSYGDPANTFKVGSAAVMDAVVKYDLA
                     RFGMAGSSLAVNVNNLLDREYVASCFQTYGCFWGAERQVVATATFRF"
     gene            12422..13219
                     /gene="fhuC"
                     /locus_tag="ACHKBL_000084"
     CDS             12422..13219
                     /gene="fhuC"
                     /locus_tag="ACHKBL_000084"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe3+-hydroxamate ABC transporter ATP-binding
                     protein FhuC"
                     /protein_id="HFO5817014.1"
                     /translation="MQEYTNHSDTTFALRNISFRVPGRTLLHPLSLTFPAGKVTGLIG
                     HNGSGKSTLLKMLGRHQPPSEGEILLDAQPLESWSSKAFARKVAYLPQQLPPAEGMTV
                     RELVAIGRYPWHGALGRFGAADREKVEEAISLVGLKPLAHRLVDSLSGGERQRAWIAM
                     LVAQDSRCLLLDEPTSALDIAHQVDVLALVHRLSQERGLTVIAVLHDINMAARYCDYL
                     VALRGGEMIAQGTPAEIMRGETLEMIYGIPMGILPHPAGAAPVSFVY"
     gene            13219..14109
                     /gene="fhuD"
                     /locus_tag="ACHKBL_000085"
     CDS             13219..14109
                     /gene="fhuD"
                     /locus_tag="ACHKBL_000085"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter
                     substrate-binding protein FhuD"
                     /protein_id="HFO5817015.1"
                     /translation="MSGLPLISRRRLLTAMALSPLLWQMNTAHAAIIDPNRIVALEWL
                     PVELLLALGIVPYGVADTINYRLWVSEPPLPDSVIDVGLRTEPNLELLTEMKPSFMIW
                     SAGYGPSPEMLARIAPGRGFNFSDGKQPLAMARKSLTEMADLLNLQSAAETHLAQYED
                     FIRSMKPRFVKRGARPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWG
                     STAVSIDRLAAYKDVDVLCFDHDNSKDMDALMATPLWQAMPFVRAGRFQRVPAVWFYG
                     ATLSAMHFVRILDNAIGGKA"
     gene            14106..16088
                     /gene="fhuB"
                     /locus_tag="ACHKBL_000086"
     CDS             14106..16088
                     /gene="fhuB"
                     /locus_tag="ACHKBL_000086"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter permease
                     FhuB"
                     /protein_id="HFO5817016.1"
                     /translation="MSKRIALFPALLLALLVIVATALTWMNFSQALPRSQWAQAAWSP
                     DIDVIEQMIFHYSLLPRLAISLLVGAGLGLVGVLFQQVLRNPLAEPTTLGVATGAQLG
                     ITVTTLWAIPGAMASQFAALAGACVVGLIVFGVAWGKRLSPVTLILAGLVVSLYCGAI
                     NQLLVIFHHDQLQSMFLWSTGTLTQTDWGGVERLWPQLLGGVMLTLLLLRPLTLMGLD
                     DGVARNLGLALSLARLAALSLAIVISALLVNAVGIIGFIGLFAPLLAKMLGARRLLPR
                     LMLASLIGALILWLSDQIILWLTRVWMEVSTGSVTALIGAPLLLWLLPRLRSISAPDM
                     KVNDRVAAERQHVLAFALAGGVLLLMAVVVALSFGRDAHGWTWASGALLEDLMPWRWP
                     RIMAALFAGVMLAVAGCIIQRLTGNPMASPEVLGISSGAAFGVVLMLFLVPGNAFGWL
                     LPAGSLGAAVTLLIIMIAAGRGGFSPHRMLLAGMALSTAFTMLLMMLQASGDPRMAQV
                     LTWISGSTYNATDAQVWRTGIVMVILLAINPLCRRWLTILPLGGDTARAVGMALTPTR
                     IALLLLAACLTATATMTIGPLSFVGLMAPHIARMMGFRRTMPHIVISALVGGLLLVFA
                     DWCGRMVLFPFQIPAGLLSTFIGAPYFIYLLRKQSR"
     gene            complement(16123..17403)
                     /gene="hemL"
                     /locus_tag="ACHKBL_000087"
     CDS             complement(16123..17403)
                     /gene="hemL"
                     /locus_tag="ACHKBL_000087"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HFO5817017.1"
                     /translation="MSKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYL
                     YDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLVTEL
                     VPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
                     GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPHEIACIIVEPVAGNMNCVPPLPE
                     FLPGLRALCDEFGALLIIDEVMTGFRVALAGAQDYYGVVPDLTCLGKIIGGGMPVGAF
                     GGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGVHETLDELTTRLAEG
                     LLEAAEEAGIPLVVNHVGGMFGIFFTDAESVTCYQDVMACDVERFKRFFHMMLDEGVY
                     LAPSAFEAGFMSVAHSMEDINNTIDAARRVFAKL"
     gene            17569..17616
                     /gene="yadX"
                     /locus_tag="ACHKBL_000088"
     CDS             17569..17616
                     /gene="yadX"
                     /locus_tag="ACHKBL_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YadX"
                     /protein_id="HFO5817018.1"
                     /translation="MERASKMPSSYLYDQ"
     gene            17628..19049
                     /gene="clcA"
                     /locus_tag="ACHKBL_000089"
     CDS             17628..19049
                     /gene="clcA"
                     /locus_tag="ACHKBL_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HFO5817019.1"
                     /translation="MKTDTPSLETPQAARLRRRQLIRQLLERDKTPLAILFMAAVVGT
                     LVGLAAVAFDKGVAWLQNQRMGALVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAP
                     EAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGGLGTLGGGMVLGREGPTVQIGGNIGR
                     MVLDVFRLKGDEARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAV
                     FIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGM
                     QDLLHRVHGGNITKWVLMGGAIGGLCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLV
                     FIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVAVELFPQYHLEAGTFAIA
                     GMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLLAQFTGGKPLYSAILA
                     RTLAKQEAEQLARSKAASASENT"
     gene            19073..19138
                     /gene="yadW"
                     /locus_tag="ACHKBL_000090"
     CDS             19073..19138
                     /gene="yadW"
                     /locus_tag="ACHKBL_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small protein YadW"
                     /protein_id="HFO5817020.1"
                     /translation="MAIIIGLEFAQLPMSFGAKYE"
     gene            19131..19475
                     /gene="erpA"
                     /locus_tag="ACHKBL_000091"
     CDS             19131..19475
                     /gene="erpA"
                     /locus_tag="ACHKBL_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HFO5817021.1"
                     /translation="MSDDVALPLEFTDAAANKVKSLIADEDNPNLKLRVYITGGGCSG
                     FQYGFTFDDQVNEGDMTIEKQGVGLVVDPMSLQYLVGGSVDYTEGLEGSRFIVTNPNA
                     KSTCGCGSSFSI"
     gene            complement(19522..20145)
                     /gene="yadS"
                     /locus_tag="ACHKBL_000092"
     CDS             complement(19522..20145)
                     /gene="yadS"
                     /locus_tag="ACHKBL_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HFO5817022.1"
                     /translation="MLVYWLDIVGTAVFAISGVLLAGKLRMDPFGVLVLGVVTAVGGG
                     TIRDMALDHGPVFWVKDPTDLVVAMVTSMLTIVLVRQPRRLPKWMLPVLDAVGLAVFV
                     GIGVNKAFNAEAGPLIAVCMGVITGVGGGIIRDVLAREIPMILRTEIYATACIIGGIV
                     HATAYYTFSVPLETASMMGMVVTLLIRLAAIRWHLKLPTFALDENGR"
     gene            complement(20183..20983)
                     /gene="btuF"
                     /locus_tag="ACHKBL_000093"
     CDS             complement(20183..20983)
                     /gene="btuF"
                     /locus_tag="ACHKBL_000093"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter substrate-binding
                     protein BtuF"
                     /protein_id="HFO5817023.1"
                     /translation="MAKSLFRALVALSFLAPLWLNAAPRVITLSPANTELAFAAGITP
                     VGVSSYSDYPPQAQKIEQVSTWQGMNLERIVALKPDLVIAWRGGNAERQVDQLASLGI
                     KVMWVDATSIEQIANALRQLVPWSPQPDKAEQAAQSLLDQYAQLKAQYADKPKKRVFL
                     QFGINPPFTSGKESIQNQVLEVCGGENIFKDSRVPWPQVSREQVLARAPQAIVITGGP
                     DQIPKIKQYWGEQLKIPVIPLTSDWFERASPRIILAAQQLCNALSQVD"
     gene            complement(20976..21674)
                     /gene="mtnN"
                     /locus_tag="ACHKBL_000094"
     CDS             complement(20976..21674)
                     /gene="mtnN"
                     /locus_tag="ACHKBL_000094"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HFO5817024.1"
                     /translation="MKIGIIGAMEEEVTLLRDKIENRQTISLGGCEIYTGQLNGTEVA
                     LLKSGIGKVAAALGATLLLEHCKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDADV
                     TAFGYEYGQLPGCPAGFKADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAK
                     IRHNFPQAIAVEMEATAIAHVCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQS
                     SLMVESLVQKLAHG"
     gene            21758..23275
                     /gene="dgt"
                     /locus_tag="ACHKBL_000095"
     CDS             21758..23275
                     /gene="dgt"
                     /locus_tag="ACHKBL_000095"
                     /EC_number="3.1.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008832 - dGTPase activity [Evidence IEA];
                     GO_process: GO:0006203 - dGTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HFO5817025.1"
                     /translation="MAQIDFRKKINWHRRYRSPQGVKTEHEILRIFESDRGRIINSPA
                     IRRLQQKTQVFPLERNAAVRTRLTHSMEVQQVGRYIAKEILSRLKELKLLEAYGLDEL
                     TGPFESIVEMSCLMHDIGNPPFGHFGEAAINDWFRQRLHPEDAESQPLTDDRCSVAAL
                     RLRDGEEPLNELRRKIRQDLCHFEGNAQGIRLVHTLMRMNLTWAQVGGILKYTRPAWW
                     RGETPETHHYLMKKPGYYLSEEAYIARLRKELNLALYSRFPLTWIMEAADDISYCVAD
                     LEDAVEKRIFTVEQLYHHLHEAWGQHEKGSLYSLVVENAWEKSRSNSLSRSTEDQFFM
                     YLRVNTLNKLVPYAAQRFIDNLPAIFAGTFNHALLEDASECSDLLKLYKNVAVKHVFS
                     HPDVEQLELQGYRVISGLLEIYRPLLSLSLSDFTELVEKERVKRFPIESRLFNKLSTR
                     HRLAYVEAVSKLPSDSPEFPLWEYYYRCRLLQDYISGMTDLYAWDEYRRLMAVEQ"
     gene            23405..24829
                     /gene="degP"
                     /locus_tag="ACHKBL_000096"
     CDS             23405..24829
                     /gene="degP"
                     /locus_tag="ACHKBL_000096"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegP"
                     /protein_id="HFO5817026.1"
                     /translation="MKKTTLALSALALSLGLALSPLSATAAETSSATTAQQMPSLAPM
                     LEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGQGGN
                     GGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLSDGRKFDAKMVGKDPRS
                     DIALIQIQNPKNLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNA
                     ENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNL
                     TSQMVEYGQVKRGELGIMGTELNSELAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGD
                     VITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSSS
                     IFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKV
                     LDSKPSVLALNIQRGDSTIYLLMQ"
     gene            24984..26141
                     /gene="cdaR"
                     /locus_tag="ACHKBL_000097"
     CDS             24984..26141
                     /gene="cdaR"
                     /locus_tag="ACHKBL_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CdaR"
                     /protein_id="HFO5817027.1"
                     /translation="MAGWHLDTKMAQDIVARTMRIIDTNINVMDARGRIIGSGDRERI
                     GELHEGALLVLSQGRVVDIDDAVARHLHGVRQGINLPLRLEGEIVGVIGLTGEPENLR
                     KYGELVCMTAEMMLEQSRLMHLLAQDSRLREELVMNLIQAEENTPALTEWAQRLGIDL
                     NQPRVVAIVEVDSGQLGVDSAMAELQQLQNALTTPERNNLVAIVSLTEMVVLKPALNS
                     FGRWDAEDHRKRVEQLITRMKEYGQLRFRVSLGNYFTGPGSIARSYRTAKTTMVVGKQ
                     RMPESRCYFYQDLMLPVLLDSLRGDWQANELARPLARLKAMDNNGLLRRTLAAWFRHN
                     VQPLATSKALFIHRNTLEYRLNRISELTGLDLGNFDDRLLLYVALQLDEQR"
     gene            complement(26256..26642)
                     /gene="yaeH"
                     /locus_tag="ACHKBL_000098"
     CDS             complement(26256..26642)
                     /gene="yaeH"
                     /locus_tag="ACHKBL_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3461 family protein"
                     /protein_id="HFO5817028.1"
                     /translation="MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAK
                     SVKFKYPRQRKTVVADGIGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILD
                     DLRHLESVVTNKISEIEADLEKLTRK"
     gene            complement(26800..27612)
                     /gene="yaeI"
                     /locus_tag="ACHKBL_000099"
     CDS             complement(26800..27612)
                     /gene="yaeI"
                     /locus_tag="ACHKBL_000099"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase YaeI"
                     /protein_id="HFO5817029.1"
                     /translation="MITRRRFLQAAAATLAAGSSLSYMHYWEPGWLELIRHRLAFFKD
                     NAAPFKILFLADLHYSRFVPLSLISDAIALGVAQKPDLILLGGDYVLFDMQLNFSAFS
                     DVLSSLAECAPTFACYGNHDRPVGTEKNRLIGEALKSAGITVLFNEAMLITTQKQQFE
                     LVGTGDLWTGQCKPPPASEANLPRLVLAHNPDSKEVMRNEKWDLMLCGHTHGGQLRIP
                     LVGEPFAPIEDKRYAAGLNAFGERQIYTTRGVGSLYGLRLNCRPEVTILELV"
     gene            complement(27665..28489)
                     /gene="dapD"
                     /locus_tag="ACHKBL_000100"
     CDS             complement(27665..28489)
                     /gene="dapD"
                     /locus_tag="ACHKBL_000100"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HFO5817030.1"
                     /translation="MQQLQNIIETAFERRAEITPANADTVTREAVNQVIALLDSGALR
                     VAEKIDGQWVTHQWLKKAVLLSFRINDNQVIEGAESRYFDKVPMKFADYDEARFQKEG
                     FRVVPPAAVRQGAFIARNTVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVIVEEGSVISMGVYIGQSTRIYDRE
                     TGEIHYGRVPAGSVVVSGNLPSKDGKYSLYCAVIVKKVDAKTRGKVGINELLRTID"
     gene            complement(28520..31192)
                     /gene="glnD"
                     /locus_tag="ACHKBL_000101"
     CDS             complement(28520..31192)
                     /gene="glnD"
                     /locus_tag="ACHKBL_000101"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HFO5817031.1"
                     /translation="MNTLPEQYANTALPTLPGQPQNPCAWPRDELTVCGIKAHIDTFQ
                     RWLGDAFDNGISAEQLIEARTEFIDQLLQRLWIEAGFSQIADLALVAVGGYGRGELHP
                     LSDIDLLILSRKKLPDDQAQKVGELLTLLWDVKLEVGHSVRTLEECMLEGLSDLTVAT
                     NLIESRLLIGDVALFLELQKHIFSEGFWPSDKFYAAKVEEQNQRHQRYHGTSYNLEPD
                     IKSSPGGLRDIHTLQWVARRHFGATSLDEMVGFGFLTSAERAELNECLHILWRIRFAL
                     HLVVSRYDNRLLFDRQLSVAQRLNYSGEGNEPVERMMKDYFRVTRRVSELNQMLLQLF
                     DEAILALPADEKPRPIDDEFQLRGTLIDLRDETLFMRQPEAILRMFYTMVRNSAITGI
                     YSTTLRQLRHARRHLQQPLCNIPEARKLFLSILRHPGAVRRGLLPMHRHSVLGAYMPQ
                     WSHIVGQMQFDLFHAYTVDEHTIRVMLKLESFASEETRQRHPLCVDVWPRLPSTELIF
                     IAALFHDIAKGRGGDHSILGAQDVVHFAELHGLNSRETQLVAWLVRQHLLMSVTAQRR
                     DIQDPEVIKQFAEEVQTENRLRYLVCLTVADICATNETLWNSWKQSLLRELYFATEKQ
                     LRRGMQNTPDMRERVRHHQLQALALLRMDNIDEEALHQIWSRCRANYFVRHSPNQLAW
                     HARHLLQHDLSKPLVLLSPQATRGGTEIFIWSPDRPYLFAAVCAELDRRNLSVHDAQI
                     FTTRDGMAMDTFIVLEPDGSPLSADRHEVIRFGLEQVLTQSSWQPPQPRRQPAKLRHF
                     TVETEVTFLPTHTDRKSFLELIALDQPGLLARVGKIFADLGISLHGARITTIGERVED
                     LFIIATADRRALNNELQQEVHQRLTEALNPNDKG"
     gene            complement(31254..32048)
                     /gene="map"
                     /locus_tag="ACHKBL_000102"
     CDS             complement(31254..32048)
                     /gene="map"
                     /locus_tag="ACHKBL_000102"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HFO5817032.1"
                     /translation="MAISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRI
                     CNDYIVNEQHAVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIK
                     DGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFVEAE
                     GFSVVREYCGHGIGRGFHEEPQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMK
                     DGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE"
     gene            32416..33141
                     /gene="rpsB"
                     /locus_tag="ACHKBL_000103"
     CDS             32416..33141
                     /gene="rpsB"
                     /locus_tag="ACHKBL_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HFO5817033.1"
                     /translation="MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLE
                     KTVPMFNEALAELNKIASRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQSQDGTFDKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
                     LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVA
                     ATVREGRSQDLASQAEESFVEAE"
     gene            33399..34250
                     /gene="tsf"
                     /locus_tag="ACHKBL_000104"
     CDS             33399..34250
                     /gene="tsf"
                     /locus_tag="ACHKBL_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HFO5817034.1"
                     /translation="MAEITASLVKELRERTGAGMMDCKKALTEANGDIELAIENMRKS
                     GAIKAAKKAGNVAADGVIKTKIDGNYGIILEVNCQTDFVAKDAGFQAFADKVLDAAVA
                     GKITDVEVLKAQFEEERVALVAKIGENINIRRVAALEGDVLGSYQHGARIGVLVAAKG
                     ADEELVKHIAMHVAASKPEFIKPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEG
                     RMKKFTGEVSLTGQPFVMEPSKTVGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEV
                     AAMSKQS"
     gene            34397..35122
                     /gene="pyrH"
                     /locus_tag="ACHKBL_000105"
     CDS             34397..35122
                     /gene="pyrH"
                     /locus_tag="ACHKBL_000105"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HFO5817035.1"
                     /translation="MATNAKPVYKRILLKLSGEALQGTEGFGIDASILDRMAQEIKEL
                     VELGIQVGVVIGGGNLFRGAGLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNA
                     RLMSAIPLNGVCDSYSWAEAISLLRNNRVVILSAGTGNPFFTTDSAACLRGIEIEADV
                     VLKATKVDGVFTADPAKDPTATMYEQLTYSEVLEKELKVMDLAAFTLARDHKLPIRVF
                     NMNKPGALRRVVMGEKEGTLITE"
     gene            35414..35971
                     /gene="frr"
                     /locus_tag="ACHKBL_000106"
     CDS             35414..35971
                     /gene="frr"
                     /locus_tag="ACHKBL_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HFO5817036.1"
                     /translation="MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEY
                     YGTPTPLRQLASVTVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPL
                     PPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDV
                     QKLTDAAIKKIEAALADKEAELMQF"
     gene            36063..37259
                     /gene="ispC"
                     /locus_tag="ACHKBL_000107"
     CDS             36063..37259
                     /gene="ispC"
                     /locus_tag="ACHKBL_000107"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HFO5817037.1"
                     /translation="MKQLTILGSTGSIGCSTLDVVRHNPEHFRVVALVAGKNVTRMVE
                     QCLEFSPRYAVMDDEASAKLLKTMLQQQGSRTEVLSGQQAACDMAALEDVDQVMAAIV
                     GAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVKQSKAQLLPVDSEHNAIFQSL
                     PQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKI
                     SVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDM
                     RTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYDRYPCLKLAMEAFEQGQAATTA
                     LNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDANAREVARKE
                     VMRLAS"
     gene            37448..38206
                     /gene="ispU"
                     /locus_tag="ACHKBL_000108"
                     /gene_synonym="uppS"
     CDS             37448..38206
                     /gene="ispU"
                     /locus_tag="ACHKBL_000108"
                     /gene_synonym="uppS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002094 - polyprenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008834 -
                     di-trans,poly-cis-undecaprenyl-diphosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0043164 - Gram-negative-bacterium-type cell
                     wall biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl-diphosphate-specific
                     ditrans,polycis-undecaprenyl-diphosphate synthase"
                     /protein_id="HFO5817038.1"
                     /translation="MLSATQPLSEKLPAHGCRHVAIIMDGNGRWAKKQGKIRAFGHKA
                     GAKSVRRAVSFAANNGIEALTLYAFSSENWNRPAQEVSALMELFVWALDSEVKSLHRH
                     NVRLRIIGDTSRFNSRLQERIRKSEALTAGNTGLTLNIAANYGGRWDIVQGVRQLAEK
                     VQQGNLQPDQIDEEMLNQHVCMHELAPVDLVIRTGGEHRISNFLLWQIAYAELYFTDV
                     LWPDFDEQDFEGALNAFANRERRFGGTEPGDETA"
     gene            38219..39076
                     /gene="cdsA"
                     /locus_tag="ACHKBL_000109"
     CDS             38219..39076
                     /gene="cdsA"
                     /locus_tag="ACHKBL_000109"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFO5817039.1"
                     /translation="MLKYRLISAFVLIPVVIAALFLLPPVGFAIVTLVVCMLAAWEWG
                     QLSGFTTRSQRVWLAVLCGLLLALMLFLLPEYHRNIHQPLVEISLWASLGWWIVALLL
                     VLFYPGSAAIWRNSKTLRLIFGVLTIVPFFWGMLALRAWHYDENHYSGAIWLLYVMIL
                     VWGADSGAYMFGKLFGKHKLAPKVSPGKTWQGFIGGLATAAVISWGYGMWANLDVAPV
                     TLLICSIVAALASVLGDLTESMFKREAGIKDSGHLIPGHGGILDRIDSLTAAVPVFAC
                     LLLLVFRTL"
     gene            39088..40440
                     /gene="rseP"
                     /locus_tag="ACHKBL_000110"
     CDS             39088..40440
                     /gene="rseP"
                     /locus_tag="ACHKBL_000110"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HFO5817040.1"
                     /translation="MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGF
                     GKALWRRTDKLGTEYVIALIPLGGYVKMLDERAEPVVPELRHHAFNNKSVGQRAAIIA
                     AGPVANFIFAIFAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
                     DWDAVRLQLVDKIGDESTTITVAPFGSDQRRDVKLDLRHWAFEPDKEDPVTSLGIRPR
                     GPQIEPVLENVQPNSAASKAGLQAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEI
                     ERQGSPLSLTLIPESKPGNGKAIGFVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKT
                     WQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGI
                     INLFPLPVLDGGHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL
                     "
     gene            40470..42902
                     /gene="bamA"
                     /locus_tag="ACHKBL_000111"
     CDS             40470..42902
                     /gene="bamA"
                     /locus_tag="ACHKBL_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0071709 - membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HFO5817041.1"
                     /translation="MAMKKLLIASLLFSSATVYGAEGFVVKDIHFEGLQRVAVGAALL
                     SMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGN
                     KSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPR
                     NRVDLKLVFQEGVSAEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQK
                     LAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEGDQYKLSGVEVSGN
                     LAGHSAEIEQLTKIEPGELYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKT
                     VKLRVNVDAGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRL
                     GFFETVDTDTQRVPGSPDQVDVVYKVKERNTGSFNFGIGYGTESGVSFQAGVQQDNWL
                     GTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKS
                     YGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFK
                     TDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDD
                     HKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNY
                     DPDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFITPTPFISDKYANSVRTSFFWD
                     MGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDK
                     AEQFQFNIGKTW"
     gene            43024..43509
                     /gene="skp"
                     /locus_tag="ACHKBL_000112"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
     CDS             43024..43509
                     /gene="skp"
                     /locus_tag="ACHKBL_000112"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Skp"
                     /protein_id="HFO5817042.1"
                     /translation="MKKWLLAAGLGLALATSAQAADKIAIVNMGSLFQQVAQKTGVSN
                     TLENEFKGRASELQRMETDLQAKMKKLQSMKAGSDRTKLEKDVMAQRQTFAQKAQAFE
                     QDRARRSNEERGKLVTRIQTAVKSVANSQDIDLVVDANAVAYNSSDVKDITADVLKQV
                     K"
     gene            43513..44538
                     /gene="lpxD"
                     /locus_tag="ACHKBL_000113"
     CDS             43513..44538
                     /gene="lpxD"
                     /locus_tag="ACHKBL_000113"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HFO5817043.1"
                     /translation="MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVN
                     PKYREHLGLCQASAVVMTQDDLPFAKSAALVVKNPYLTYARMAQILDTTPQPAQNIAP
                     SAVIDATAKLGNNVSIGANAVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTI
                     YHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRG
                     ALDDTVIGNGVIIDNQCQIAHNVVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGH
                     MEICDKVTVTGMGMVMRPITEPGVYSSGIPLQPNKVWRKTAALVMNIDDMSKRLKSLE
                     RKVNQQD"
     gene            44643..45098
                     /gene="fabZ"
                     /locus_tag="ACHKBL_000114"
     CDS             44643..45098
                     /gene="fabZ"
                     /locus_tag="ACHKBL_000114"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFO5817044.1"
                     /translation="MTTNTHTLQIEEILELLPHRFPFLLVDRVLDFEEGRFLRAVKNV
                     SVNEPFFQGHFPGKPIFPGVLILEAMAQATGILAFKSVGKLEPGELYYFAGIDEARFK
                     RPVVPGDQMIMEVTFEKTRRGLTRFKGVALVDGKVVCEATMMCARSREA"
     gene            45102..45890
                     /gene="lpxA"
                     /locus_tag="ACHKBL_000115"
     CDS             45102..45890
                     /gene="lpxA"
                     /locus_tag="ACHKBL_000115"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HFO5817045.1"
                     /translation="MIDKSAFVHPTAIVEEGASIGANAHIGPFCIVGPHVEIGEGTVL
                     KSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGEPTRVEIGDRNRIRESVTIHRGT
                     VQGGGLTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMTAVH
                     QFCIIGAHVMVGGCSGVAQDVPPYVIAQGNHATPFGVNIEGLKRRGFSREAITAIRNA
                     YKLIYRSGKTLDEVKPEIAELAETYPEVKAFTDFFARSTRGLIR"
     gene            45890..47038
                     /gene="lpxB"
                     /locus_tag="ACHKBL_000116"
     CDS             45890..47038
                     /gene="lpxB"
                     /locus_tag="ACHKBL_000116"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HFO5817046.1"
                     /translation="MTEQRPLTIALVAGETSGDILGAGLIRALKERVPNARFVGVAGP
                     RMQAEGCEAWYEMEELAVMGIVEVLGRLRRLLHIRADLTTRFGELKPDVFVGIDAPDF
                     NITLEGNLKKQGIKTIHYVSPSVWAWRQKRVFKIGRATDLVLAFLPFEKAFYDKYNVP
                     CRFIGHTMADAMPLDPDKNAARDVLGIPHDAHCLALLPGSRGAEVEMLSADFLKTAQL
                     LRQTYPDLEIVVPLVNAKRREQFERIKAEVAPDLSVHMLDGMGREAMVASDAALLASG
                     TAALECMLAKCPMVVGYRMKPFTFWLAKRLVKTDYVSLPNLLAGRELVKELLQEECEP
                     QKLAAALLPLLANGKTSHAMHDTFRELHQQIRCNADEQAAQAVLELAQ"
     gene            47035..47631
                     /gene="rnhB"
                     /locus_tag="ACHKBL_000117"
     CDS             47035..47631
                     /gene="rnhB"
                     /locus_tag="ACHKBL_000117"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HFO5817047.1"
                     /translation="MIEFVYPHTHLVAGVDEVGRGPLVGAVVTAAVILDPARPIAGLN
                     DSKKLSEKRRLALCEEIKEKALSWSLGRAEPHEIDELNILHATMLAMQRAVAGLHIAP
                     EYVLIDGNRCPKLPMPAMAVVKGDSRVPEISAASILAKVTRDAEMAALDIVFPQYGFA
                     QHKGYPTAFHLEKLAEYGATEHHRRSFGPVKRVLGLAS"
     gene            47668..51150
                     /gene="dnaE"
                     /locus_tag="ACHKBL_000118"
     CDS             47668..51150
                     /gene="dnaE"
                     /locus_tag="ACHKBL_000118"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HFO5817048.1"
                     /translation="MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDF
                     TNLCGLVKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLTLLIS
                     KAYQRGYGAAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNSALVDECVAFYEE
                     HFPDRYFLELIRTGRPDEESYLHAAVELAEARGLPVVATNDVRFIDSSDFDAHEIRVA
                     IHDGFTLDDPKRPRNYSPQQYMRSEEEMCELFADIPEALANTVEIAKRCNVTVRLGEY
                     FLPQFPTGDMSTEDYLVKRAKEGLEERLAFLFPDEEERVKRRPEYDERLETELQVINQ
                     MGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFL
                     NPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMAAKAVIRDVGRVLG
                     HPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPEIYEADEEVKALIDMARKLEGVTRNA
                     GKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAGLVKFDFLGLRTLTIIN
                     WALEMINKRRAKNGEPPLDIAAIPLDDKKSFDMLQRSETTAVFQLESRGMKDLIKRLQ
                     PDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREEISYPDVQWQHESLKPVLEPTYG
                     IILYQEQVMQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGINAEL
                     AMKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTADMDNTEKVV
                     GLVDECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGAIKGVGEGPIEAIIEARNKG
                     GYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHRAALMNSLGDALKAADQHAK
                     AEAIGQADMFGVLAEEPEQIEQSYASCQPWPEQVVLDGERETLGLYLTGHPINQYLKE
                     IERYVGGVRLKDMHPTERGKVITAAGLVVAARVMVTKRGNRIGICTLDDRSGRLEVML
                     FTDALDKYQQLLEKDRILIVSGQVSFDDFSGGLKMTAREVMDIDEAREKYARGLAISL
                     TDRQIDDQLLNRLRQSLEPHRSGTIPVHLYYQRADARARLRFGATWRVSPSDRLLNDL
                     RGLIGSEQVELEFD"
     gene            51163..52122
                     /gene="accA"
                     /locus_tag="ACHKBL_000119"
     CDS             51163..52122
                     /gene="accA"
                     /locus_tag="ACHKBL_000119"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HFO5817049.1"
                     /translation="MSLNFLDFEQPIAELEAKIDSLTAVSRQDEKLDINIDEEVHRLR
                     EKSVELTRKIFADLGAWQIAQLARHPQRPYTLDYVRLAFDEFDELAGDRAYADDKAIV
                     GGIARLDGRPVMIIGHQKGRETKEKIRRNFGMPAPEGYRKALRLMQMAERFKMPIITF
                     IDTPGAYPGVGAEERGQSEAIARNLREMSRLGVPVVCTVIGEGGSGGALAIGVGDKVN
                     MLQYSTYSVISPEGCASILWKSADKAPLAAEAMGIIAPRLKELKLIDSIIPEPLGGAH
                     RNPEAMAASLKAQLLADLADLDVLSTEDLKNRRYQRLMSYGYA"
     gene            52221..54362
                     /gene="ldcC"
                     /locus_tag="ACHKBL_000120"
     CDS             52221..54362
                     /gene="ldcC"
                     /locus_tag="ACHKBL_000120"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HFO5817050.1"
                     /translation="MNIIAIMGPHGVFYKDEPIKELESALVAQGFQIIWPQNSVDLLK
                     FIEHNPRICGVIFDWDEYSLDLCSDINQLNEYLPLYAFINTHSTMDVSVQDMRMALWF
                     FEYALGQAEDIAIRMRQYTDEYLDNITPPFTKALFTYVKERKYTFCTPGHMGGTAYQK
                     SPVGCLFYDFFGGNTLKADVSISVTELGSLLDHTGPHLEAEEYIARTFGAEQSYIVTN
                     GTSTSNKIVGMYAAPSGSTLLIDRNCHKSLAHLLMMNDVVPVWLKPTRNALGILGGIP
                     RREFTRDSIEEKVAATTQAQWPVHAVITNSTYDGLLYNTDWIKQTLDVPSIHFDSAWV
                     PYTHFHPIYQGKSGMSGERVAGKVIFETQSTHKMLAALSQASLIHIKGEYDEEAFNEA
                     FMMHTTTSPSYPIVASVETAAAMLRGNPGKRLINRSVERALHFRKEVQRLREESDGWF
                     FDIWQPPQVDEAECWPVAPGERWHGFNDADADHMFLDPVKVTILTPGMDEQGNMSEEG
                     IPAALVAKFLDERGIVVEKTGPYNLLFLFSIGIDKTKAMGLLRGLTEFKRSYDLNLRI
                     KNMLPDLYAEDPDFYRNMRIQDLAQGIHKLIRKHDLSGLMLRAFDTLPEMIMTPHQAW
                     QRQIKGEVETIALEQLVGRVSANMILPYPPGVPLLMPGEMLTKESRTVLDFLLMLCSV
                     GQHYPGFETDIHGAKQDEDGVYRVRVLKMAG"
     gene            54419..54808
                     /gene="yaeR"
                     /locus_tag="ACHKBL_000121"
     CDS             54419..54808
                     /gene="yaeR"
                     /locus_tag="ACHKBL_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFO5817051.1"
                     /translation="MLGLKQVHHIAIIATDYAVSKAFYCDILGFTLQSEVYREARDSW
                     KGDLALNGQYVIELFSFPFPPERPSRPEACGLRHLAFSVDDIDAAVAHLESHNVKCEA
                     IRVDPYTQKRFTFFNDPDGLPLELYEQ"
     gene            54873..56171
                     /gene="tilS"
                     /locus_tag="ACHKBL_000122"
     CDS             54873..56171
                     /gene="tilS"
                     /locus_tag="ACHKBL_000122"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HFO5817052.1"
                     /translation="MTLTLNRQLLSSRQILVAFSGGLDSTVLLHQLVQWRMENPGVTL
                     RAIHVHHGLSANADAWVRHCENICQQWQVPLVVERVQLAQEGLGIEAQARQARYQAFA
                     RTLLPGEVLVTAQHLDDQCETFLLALKRGSGPAGLSAMGEVSEFAGTRLIRPLLARTR
                     GELVQWARQYDLRWIEDESNQDDSYDRNFLRLRVVPLLQQRWPHFAEATARSAALCAE
                     QESLLDELLADDIAHCQTSQGTLQIAPMLAMSDARRAAIIRRWLAGKNAPMPSRDALV
                     RIWQEVALAREDASPCLRLGAFEIRRYQSQLWWIKSVTGQSETIVPWQTWLQPLELPA
                     GLGSVQLTAGGDIRPPRADEAVSVRFKAPGLLHIIGRNGGRKLKKIWQELGVPPWLRD
                     TTPLLFYGETLIAAAGVFVTQEGVAEGENGVSFVWQKTLS"
     gene            complement(56220..56480)
                     /gene="rof"
                     /locus_tag="ACHKBL_000123"
     CDS             complement(56220..56480)
                     /gene="rof"
                     /locus_tag="ACHKBL_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho-binding antiterminator"
                     /protein_id="HFO5817053.1"
                     /translation="MSMNDTYQPINCDDYDNLELACQHHLMLTLELKDGEKLQAKASD
                     LVSRKNVEYLVVEAAGTTRELRLDKITSFSHPEIGTVVVSES"
     gene            complement(56467..56667)
                     /gene="yaeP"
                     /locus_tag="ACHKBL_000124"
     CDS             complement(56467..56667)
                     /gene="yaeP"
                     /locus_tag="ACHKBL_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeP family protein"
                     /protein_id="HFO5817054.1"
                     /translation="MEKYCELIRKRYAEIASGDLGYVPDALGCVLKVLNEMAADDALS
                     EAVREKAAYAAANLLVSDYVNE"
     gene            56833..57378
                     /gene="yaeQ"
                     /locus_tag="ACHKBL_000125"
     CDS             56833..57378
                     /gene="yaeQ"
                     /locus_tag="ACHKBL_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HFO5817055.1"
                     /translation="MALKATIYKATVNVADLDRNQFLDASLTLARHPSETQERMMLRL
                     LAWLKYADERLQFTRGLCADDEPEAWLRNDHLGIDLWIELGLPDERRIKKACTQAAEV
                     ALFAYNSRAAQIWWQQNQSKCAQFSNLSIWYLDDEQLAKVSAFADRTMTLQATIQDGV
                     IWLSDDKNNLEVNLTAWQQPS"
     gene            57375..57797
                     /gene="arfB"
                     /locus_tag="ACHKBL_000126"
     CDS             57375..57797
                     /gene="arfB"
                     /locus_tag="ACHKBL_000126"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HFO5817056.1"
                     /translation="MIVISRHVAIPDGELEITAIRAQGAGGQHVNKTSTAIHLRFDIR
                     ASSLPEYYKERLLAASHHLISSDGVIVIKAQEYRSQELNREAALARLVAVIKDLTTEQ
                     KARRPTRPTRASKERRLASKAQKSSVKAMRGKVRSGRE"
     gene            57811..58521
                     /gene="nlpE"
                     /locus_tag="ACHKBL_000127"
                     /gene_synonym="cutF"
     CDS             57811..58521
                     /gene="nlpE"
                     /locus_tag="ACHKBL_000127"
                     /gene_synonym="cutF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414734.1"
                     /note="NlpE, an outer membrane lipoprotein, interacts
                     directly with CpxA, the sensor histidine kinase of the Cpx
                     system for response to envelope stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response activation lipoprotein
                     NlpE"
                     /protein_id="HFO5817057.1"
                     /translation="MVKKAIVTAMAVISLFTLMGCNNRAEVDTLSPAQAAELKPMPQS
                     WRGVLPCADCEGIETSLFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTD
                     SKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEPAQSSLPMTPMTLRGMYFYMADAA
                     TFTDCATGKRFMVANNAELERSYLAARGHSEKPVLLSVEGHFTLEGNPDTGAPTKVLA
                     PDTAGKFYPNQDCSSLGQ"
     gene            complement(58551..59375)
                     /gene="yaeF"
                     /locus_tag="ACHKBL_000128"
     CDS             complement(58551..59375)
                     /gene="yaeF"
                     /locus_tag="ACHKBL_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeF family permuted papain-like enzyme"
                     /protein_id="HFO5817058.1"
                     /translation="MDKPKAHCRLLLPCFLFLSACTVDISQPDSSATAVDVEAKTWVV
                     KFQHQSSFTEQSIKEITAPDLKPGDLLFSSSLGVTSFGIRVFSTSSVSHVAIFLGDNN
                     VAEATGAGVQIVSLKKAMKHSDKLFVLRVPDLTPQQATDITAFANKIKDSGYNYRGIV
                     EFIPFMVTRQVCSLNPFSEDFRQQCVSGLAKAQLSSVGEGDKKSWFCSEFVTDAFAKA
                     GHPLTLAQSGWISPADLMHMRTGDISAFKPETQLQYVGHMKPGIYIKAGRFVGLTQ"
     gene            complement(59428..61146)
                     /gene="proS"
                     /locus_tag="ACHKBL_000129"
     CDS             complement(59428..61146)
                     /gene="proS"
                     /locus_tag="ACHKBL_000129"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HFO5817059.1"
                     /translation="MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLP
                     TGVRVLKKVENIVREEMNNAGAIEVLMPVVQPSELWQESGRWEQYGPELLRIADRGDR
                     PFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
                     YSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGE
                     DDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEK
                     TVKTLLVKAVEGSSFPLVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVKAG
                     PGSLGPVNMPIPVVIDRTVAAMSDFAAGANIDGKHYFGINWDRDVATPEIADIRNVVA
                     GDPSPDGQGTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTR
                     VVAAAIEQNYDERGIVWPDAIAPFQVAILPMNMHKSFRVQELAEKLYSELRAQGIEVL
                     LDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTGDIVEYL
                     VKQIKG"
     gene            complement(61257..61964)
                     /gene="tsaA"
                     /locus_tag="ACHKBL_000130"
     CDS             complement(61257..61964)
                     /gene="tsaA"
                     /locus_tag="ACHKBL_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HFO5817060.1"
                     /translation="MSSFQFEQIGVIRSPYKEKFAVPRQPGLVKSANGELHLIAPYNQ
                     ADAVRGLEAFSHLWILFVFHQTMEGGWRPTVRPPRLGGNARMGVFATRSTFRPNPIGM
                     SLVELKEVVCHKDSVILKLGSLDLVDGTPVVDIKPYLPFAESLPNASASYAQSAPAAE
                     MAVSFTAEVEKQLLTLEKRYPQLTLFIREVLAQDPRPAYRKGEETGKTYAVWLHDFNV
                     RWRVTDAGFEVFALEPR"
     gene            complement(61961..62365)
                     /gene="rcsF"
                     /locus_tag="ACHKBL_000131"
     CDS             complement(61961..62365)
                     /gene="rcsF"
                     /locus_tag="ACHKBL_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rcs stress response system protein RcsF"
                     /protein_id="HFO5817061.1"
                     /translation="MRALPICLVALMLSGCSMLSRSPVEPVQSTAPQPKAESAKPKVP
                     RATPVRIYTNAEELVGKPFRDLGEVSGDSCQASNQDSPPSIPTARKRMQINASKMKAN
                     AVLLHSCEVTSGTPGCYRQAVCIGSALNITAK"
     gene            complement(62483..63298)
                     /gene="metQ"
                     /locus_tag="ACHKBL_000132"
     CDS             complement(62483..63298)
                     /gene="metQ"
                     /locus_tag="ACHKBL_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HFO5817062.1"
                     /translation="MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQ
                     VAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYK
                     LVAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDG
                     VGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKDGI
                     FVEDKESPYVNLIVTREDNKDAENVKKFVQAYQSDEVYEAANKVFNGGAVKGW"
     gene            complement(63338..63991)
                     /gene="metI"
                     /locus_tag="ACHKBL_000133"
     CDS             complement(63338..63991)
                     /gene="metI"
                     /locus_tag="ACHKBL_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease MetI"
                     /protein_id="HFO5817063.1"
                     /translation="MSEPMMWLLVRGVWETLAMTFVSGFFGFVIGLPVGVLLYVTRPG
                     QIIANAKLYRTVSAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAP
                     FIARMVENALLEIPTGLIEASRAMGATPMQIVRKVLLPEALPGLVNAATITLITLVGY
                     SAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK"
     gene            complement(63984..65015)
                     /gene="metN"
                     /locus_tag="ACHKBL_000134"
     CDS             complement(63984..65015)
                     /gene="metN"
                     /locus_tag="ACHKBL_000134"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HFO5817064.1"
                     /translation="MIKLSNITKVFHQGTRTIQALNNVSLHVPAGQIYGVIGASGAGK
                     STLIRCVNLLERPTEGSVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGN
                     VALPLELDNTPKDEIKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPK
                     VLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGE
                     LIEQDTVSEVFSHPKTPLAQKFIQSTLHLDIPEDYQERLQAEPFTDCVPMLRLEFTDQ
                     SVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAITWLQEHH
                     VKVEVLGYV"
     gene            65203..65775
                     /gene="gmhB"
                     /locus_tag="ACHKBL_000135"
     CDS             65203..65775
                     /gene="gmhB"
                     /locus_tag="ACHKBL_000135"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001608862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HFO5817065.1"
                     /translation="MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFIDGVIDAMRELKK
                     MGFALVVVTNQSGIARGKFTEAQFETLTEWMDWSLADRDVDLDGIYYCPHHPQGSVEE
                     FRQVCDCRKPHPGMLLSARDYLHIDMAASYMVGDKLEDMQAAAAANVGTKVLVRTGKP
                     VTPEAENAADWVLNSLADLPQAIKKQQKPA"
BASE COUNT        15699 a        16327 c        18108 g        15864 t
ORIGIN      
        1 ttggcaataa cttgaggttg ttttcagaat atatttatat tcacacgaat aataattacc
       61 acttatctaa ttcattaaag ccttttgcct gtagagcttc aactaatgat aagccatcag
      121 gaaatagcaa ttccggcgca atttcaaaca gtggccacag cataaagccg cgatttttca
      181 tatcgtagtg cggaacggtc agacgttcgg tatttattac ttcattacca aacagcatga
      241 tgtcgagatc cagcgtgcgc ggtccccagc gttcagcttt acggacgcga ccttgctgca
      301 attcgatacg ctgtgtgtga ttgagtagct cttcaggggc aagagtggtt ttcagcgcga
      361 cggctgcatt taagtaatcg ggttgatctt gcggccccag cggtggggtg cggtaaaacg
      421 aagaaacagc aagaatgcgg ctttctggaa tatcgcctaa tgctttcagg gcagcattga
      481 cctgctccag cggagaggcc agattgctgc ctatggcaat atacgccact gtcatgcggt
      541 accctcacga cgcggtgcgc gtttgcgtgg acgacgagta cggcgacgcg gtgacggttc
      601 ttcatccagt tcgttaagca tccctttttg gtctggtggc gcggaaacct ggaactcacc
      661 ccaccatttc accagacgtt gcagttcagc gttacgctca acttcggctc gtaaggccaa
      721 caggtcgtat gccgcacgga acttaggatg ctccagcagt ttccaggcgc gtttaccctg
      781 acgtcgggac atacgcaact gcaactgcca gatatcgcgg gttaacgtcg tcagacgttt
      841 cgggatcgcc agtgaacgac aggcttcgtc cagcacgtcg ttcatcgcca gcgcgaaagc
      901 gtcgtgatag gtcaggccgc tttcctgggc gatcttctgt gccgtctcca gcagtgggta
      961 ccagaacatg gcggcaaaca ggaacgccgg gttcacgcgc atatcgttat ggatacgcgt
     1021 atcggtattc ttcagcacct gttcaatgat ccgctccatc gggctgtcgc cattttccgt
     1081 gaagtagcgg gtaatggtcg ggaacagcgg ctggaacagg tgatattcac acagcagctt
     1141 ataggtttcg taaccgtagc ccgcttgcag cagtttcaaa gattcttcaa acagacgtgc
     1201 cggtggaata tcgttcagca gggtagccag gcgagggatc ggttctgcgg tttcaggact
     1261 gatacgcata cccaatttgg cggcaaaacg taccgcgcgc agcatccgta ccggatcttc
     1321 acggtagcgc gtttccgggt taccaatcag acggataacg ccgtccttca gatccttcat
     1381 gccgccaacg taatcacgga cggtaaaatc tgccacgctg taatacaggc tgttgatagt
     1441 gaaatcgcgg cgctgggcgt cttcttcgat ggagccgaaa atgttgtcgc gcagcaacat
     1501 gccgttttgc ccgcgttggg aggtcgtgcg gtcgctgacg ttaccttcgt gatgtccacg
     1561 gaaggtcgca acttcgataa tctccgggcc aaacatcaca tgagccagac ggaaacggcg
     1621 acccaccagg cggcagttac ggaacagttt acgtacctgt tcaggcgtgg cgttagtggt
     1681 tacgtcaaaa tctttcggct ttttgccaag taacaggtcg cgcacgccac cgccaaccag
     1741 ccaggcttcg tatcccgctt tattgagcct gtacattacc ttcagggcat tttcactgat
     1801 atctttgcgg gaaatagcat gctgctcacg cgggatcacc gtcacctgtg gacgggcgac
     1861 tgcctgttca gcctcgcttt cctcgcggct tagcaccttg cggcaaaaat tagcgactcg
     1921 ggtaaaaata gtacacctcg gtagtgtcaa acatcattca ggacaaaaaa atagcggcta
     1981 atcatagctc agcatgacgc atttgagaat gttgaattta caattgccga ctcaggcacg
     2041 gcggtaagcc gccagttttt gacggctgac tgaaggattt gctcgacgct gaaatcctgc
     2101 cagtgtgctt ctgcctgctg acccagaaat tgaagtgccg cgattagtac cgggcgcgga
     2161 tcgcctttcg gcaacgcagg cgcatgattc tgcttggaaa gtttagcgcc ttgtgtgtta
     2221 agcgccagcg gtaaatgaat gtaatctggc actttccagc caaaaagctg gtacagcgag
     2281 atttgccgca ctgttggttc gatcaaatct gccccgcgga caatttcgct cacgccctgg
     2341 aagtgatcat ccaccaccac cgccaggtta taggcaaaca aaccatcacg gcgatgaata
     2401 ataaaatctt cccgcgccag tttttcgttg gcgtgaataa tgccgcgcag ctgatcagta
     2461 aatagcgtga ccggatgctg ctggcggata cgtactgcgg cgttgtctgg tccatgatgc
     2521 aattcccggc aatgaccgtc gtaaataccg ccaatgcttt gaatacgcgc gcgcgtgcag
     2581 gtgcagtaat aacttagtcc ttgctcatgt aaccaggcga gtgcttcgcg ataggcatcg
     2641 tgacgttgcg attgccagag aacgtcgccg tcccagtgca gaccgtaatg ttccagctgg
     2701 cgcaggatag tctctgcggc accgggaact tcacgaggcg ggtcgatatc ttcaatgcgt
     2761 accagccagc gaccttgccg ggcgcgagcc tgtaaatagc tgccaagcgc ggcaatcaga
     2821 gagccaaaat gaagctcgcc ggaaggagag ggggcaaagc ggccaatata gtgtgtgtct
     2881 gtcatctcgt tgaacaaaaa ataaggcggg agcatttccc gcctgtggta aacgtgatgg
     2941 aacggctgta tttagccagc catttgtttt tcgcgaattt cagccagcgt tttgcagtcg
     3001 atgcacagat cggctgtcgg gcgcgcttcc agacggcgaa taccaatttc aacaccgcag
     3061 gattcgcagt agccgaaatc ttcgtcttcc acttttttca gcgtcttctc gatctttttg
     3121 atcagcttac gctcgcgatc gcggttacgc agttcgaggc tgaactcttc ttcctgggct
     3181 gcacggtcta ccgggtccgg gaagttggct gcttcatcct gcatatgtgt aacggtgcga
     3241 tcgacttcat ccctgagttg attacgccat gcttccagaa tacgacggaa gtgcgccagc
     3301 tgggcttcat tcatatactc ttcgcccggc ttctcctgat atggttccac cccagcgatg
     3361 gcgagaatac tcagggacga tgttttacgg ttttgccctt cttgcatgtt gcttctcctt
     3421 aacacgcact atcgatcccc atgttcgggg gaaaaatgag gccgctataa atagcagatg
     3481 cttttccgga tagcaattat ctaaacgtaa cacttgacaa ctgtgtgagg aaaagcgtat
     3541 ttgcgcacgc gaccagaatg taaattaacc agttacatac tttactacaa tgtaaccggc
     3601 agtgattttt taagagccat gccttcagca gaaagttccg ctttgtaagc cagaatttct
     3661 acccccctct gttgagcttc tgacaatagt tgcgcgtatt tctcatcgat gtggcgcgcg
     3721 ggtgaaaacc gtgtaatggc tgaatgcagc acggcgaaaa agataaccgc acgctggcct
     3781 tcagccgcta cgctcatcaa ctcccgaagg tgtttctgac ctcgttcagt gaccgcatcg
     3841 ggaaaatatc cctgttcgtt ctccgctaac gtaaccgatt tcacttcaat atagcagtct
     3901 ggacgcgaat ccgcctgcaa cataaagtca atacggctgc gttcggcgcc gtattttact
     3961 tcgcttttca gcgagctata gcctgacagc tctgaaattg attcattaag gatagcctct
     4021 ttcgtcaacc tgttagccca aagcgtgttg acgcaaataa atgcgccgct ctggctttga
     4081 gtcaattccc aggtgtgtgg gtatttccgt ttggtgttgt ctgaagtcga ataccagacg
     4141 gtatcgccag gcgttgcaca actggtcatc gcacccgtat tcgggcagtg tagcgttaat
     4201 tcgcgaccat cgggtgtgat cacatcggct aaaaaacgtt tgtaacgctg aattagcgtc
     4261 gcgcgctgta gagggggaga aaattccatt acgacaatcc ttgttattgc gttagcgccc
     4321 agcgttttag cggcgtgtag cgtgtgcgtc cacgggcaaa cgaggaggcg taaagggtga
     4381 actccgtcac cgtatacgac cagttaaaac ctggcggcgg gattgtcacc gcctcgctgg
     4441 cgtcgcgcaa taaggtaata tgtggatgaa acggacgatt gctttgaaaa caaccgctgc
     4501 gggcagcctg tgaacggagc atattcgcca gctggattaa gccgcgtggt ggctgacgca
     4561 tccctaacca caccacacgc gaacgcagcc attgaccggc gtcatcaagc gtgagtgtga
     4621 aacccggttg acgaatccgc ccggctaaaa gagaaagcgc cttctctttc tcggcgctca
     4681 cttcgcctaa aaatgccaga gtcagatgca aattatcggc ggcgaccgga cgtcccgcgt
     4741 caggtgggaa gtgtgtggcg cgccagtgga taatctgttc gcggatttct gcaggtaagt
     4801 cgatagcaaa gaacagccgt tgcggttcag acatgtgagg cactcggtta tgaattaccg
     4861 cgatgctaca atgtggcgcg aagaatgtta accctctgga gcgttttgtg tcgtcgttgc
     4921 ccgttgctgc cgtcttacct gagttacttg ctgctctcga ttgtgcatcg caggtgttat
     4981 taagtgcgcc gaccggggcc gggaaatcaa cctggctgcc gctgcaactg ctggcgcatc
     5041 ccggcattaa cgggaaaatt atcctgctgg agccgcgtcg tctggcggcg cgtaacgttg
     5101 cgcaacgact ggcggagctg cttaacgaaa agccaggcga taccgttggc taccggatgc
     5161 gtgcgcaaaa ctgcgtcggg ccgaataccc gcctggaagt ggttaccgaa ggcgtgctga
     5221 cgcggatgat ccagcgtgac ccggaactga gcggtgttgg attggtgatc cttgatgaat
     5281 ttcatgagcg cagcttgcag gcggatttgg cgttggcgct gttactcgat gtgcaacaag
     5341 gtctgcgtga tgaccttaaa ctgctgatta tgtcggctac gctggacaac gaccgcttgc
     5401 agcaaatgct gccagaagcg cccgtcgtca tctcagaagg gcgctcgttt ccggttgaac
     5461 gccgttattt gccgctgccc gctcatcagc gttttgacga agccgtcgcg gttgccaccg
     5521 ccgaaatgct gcgtcaggaa agtggatcat tactgttatt tttacctggc gtcggagaaa
     5581 ttcagcgtgt gcaggaacaa ctggcttcac gcatcggcag tgatgtgttg ctctgcccgc
     5641 tgtatggcgc gttgtcgctg aacgatcagc gaaaagcgat cctcccggca ccgcaaggga
     5701 tgcgtaaagt ggtgctggcg accaatattg ctgaaaccag tttaaccatc gaaggtattc
     5761 gtctggtggt ggattgtgcc caggagcgtg tggcgcattt tgatccgcgt acagggctta
     5821 cgcggctggt tactcaacgc gttagtcagg cgtcgatgac gcaacgtgcc gggcgtgccg
     5881 ggcgtctgga gccgggtatc tgcctgcatt taatcgccaa agaacaagca gaacgcgctg
     5941 ccgcgcaaag tgaaccggag atcttacaaa gcgatctttc cggtttgctg atggaattac
     6001 tgcaatgggg atgcagcgat acggcgcaga tgagctggct ggatcaaccg ccaacggtga
     6061 atctacaggc cgcgaaacgg ctgttacaaa tgctgggggc gctggagggt gaacggctta
     6121 gcgcacaagg gcaaaaaatg gcagtgctgg gtagcgatcc gcgtttagcg gcaatgctgg
     6181 ttagcgcgaa gaacgacgac gaagctgcta ccgcggcaaa aattgccgcc attctcgaag
     6241 agccgccacg gatgggcaat agtgacctgg gcgtggcgtt ttcgcgcaat caacccgcct
     6301 ggcagcaacg tagtcagcaa ctgttaaaac gcttaaacgt acgcggcggt gaggcagaca
     6361 gttcgcttat cgcgccgcta cttgccgggg cgtatgccga tcgcattgct tgtcgccgtg
     6421 ggcaagatgg acgctatcaa ctggcaaacg gcatgggggc gatgctcgat gccgacgatg
     6481 cgctaagccg ctacgaatgg ttgatcgcac cgttattatt gcagggcagc gcctcgccgg
     6541 atgcgcggat tttactggcg ctaccggtcg atatcgatga attagtacaa cgctgtccgc
     6601 agctggtaca gcagtcggac accgtggaat gggatgacgc gcaaggcacg ctgaaagcct
     6661 ggcgtcggct gcaaatcggt cagttgacgg tgaaagtgca gccactggcg aaaccctcgg
     6721 aagacgagtt gcatcaggcg atgcttaacg gcatccgtga taaaggttta agcgtgctca
     6781 actggacggc ggaagcggaa cagctacgct tgcgtttgtt attcgccgca aagtggttgc
     6841 cggaatatga ctggccagcg gttgatgatg agagtttgtt ggcgacgctg gaaacgtggc
     6901 tactgccgca tatgagcggc gtgcattcac tacgcggcct gaaatcactc gacatttatc
     6961 aggcgctacg cggattactt gattggggaa tgcagcaacg tctggatagt gaattgcctg
     7021 cgcattacac tgtgccgacg ggaagccgga tcgccattcg ttatcatgaa gataaaccgc
     7081 ccgcgctggc ggtgagaatg caggagatgt ttggtgaggc caccaatccg acgatcgccc
     7141 aggggcgcgt gccgctggtg ctggagttgc tttcccctgc ccaaaggcca ttacaaatca
     7201 cacgagattt gagcgccttc tggaaaggag cgtaccgtga ggtgcaaaaa gagatgaaag
     7261 ggcgttatcc caaacatgtc tggccggacg acccggcaaa tactgcaccg acgcgacgga
     7321 cgaaaaagta ttcgtgaaga ccaaagcaaa tttgagagat atcttcttct gtcttgcaac
     7381 agaagaacag aaaatcgggc ttttgcgcct gaatattgcg gagaaaaagc atggccggga
     7441 atgaccgcga gccaattgga cgcaaaggga aaccgacgcg tccggtcaaa caaaaggtaa
     7501 gccgtcgtcg ttacgaagat gacgattacg acgattatga tgactatgag gatgaagaac
     7561 cgatgccgcg caaaggtaag ggcaaaggca aagggcgtaa gcctcgtggc aaacgcggct
     7621 ggctatggct gctgctaaaa ctggctatcg tttttgccgt gctgatcgcc atttacggcg
     7681 tttatctcga tcaaaaaatt cgcagccgta ttgatggcaa ggtctggcaa ttgcccgcgg
     7741 cagtttatgg ccgaatggtc aaccttgagc cagacatgac catcagcaag aacgaaatgg
     7801 tgaagctgct ggaggcgacc cagtatcgtc aggtgtcgaa aatgacccgt cctggcgaat
     7861 ttaccgtgca ggccaacagc attgagatga tccgccgtcc gtttgatttc ccggacagta
     7921 aagaagggca ggtgcgtgcg cgtctgacct ttgatggcga tcatctggcg acgatcgtca
     7981 atatggagaa caaccgtcag tttggtttct tccgtcttga tccgcgcctg atcaccatga
     8041 tctcctcgcc aaacggtgag cagcgtttgt ttgtgccgcg cagtggtttc ccggatttgc
     8101 tggtggatac tttgctggcg acagaagacc gccatttcta cgagcatgat gggatcagtc
     8161 tctactccat cggacgtgcg gtgctggcaa acctgaccgc cggacgcacg gtgcagggtg
     8221 cgagtacgct gacgcaacag ctggtgaaaa acctgttcct ctccagcgag cgctcttact
     8281 ggcgtaaagc gaacgaagct tacatggcgc tgatcatgga cgcgcgttac agcaaagacc
     8341 gtattcttga gctgtatatg aacgaggtgt atctcggtca gagcggcgac aacgaaatcc
     8401 gcggcttccc gctggcgagc ttgtattact ttggtcgccc agtagaagaa ttgagcctcg
     8461 accagcaggc gctgttggtg ggtatggtga aaggggcgtc catctacaat ccgtggcgta
     8521 acccgaaact ggcgttggag cgacgtaatc tggtactgcg tctgctgcaa cagcaacaga
     8581 ttattgatca agaactctat gacatgttga gtgcacgtcc gctgggggtt cagccgcgcg
     8641 gtggggtgat ctctccacag ccagccttta tgcaactggt gcgtcaggag ctgcaggcaa
     8701 aactgggcga taaggtaaaa gatctctccg gcgtgaagat cttcactacc tttgactcgg
     8761 tggcccagga cgcggcagaa aaagccgccg tggaaggcat tccggcactg aagaaacagc
     8821 gtaagttgag cgatcttgaa accgcgattg tggtcgtcga ccgctttagt ggtgaagttc
     8881 gtgcgatggt tggaggttct gagccgcagt ttgcgggcta caaccgtgcg atgcaggcgc
     8941 gtcgttcgat tggttccctt gcaaaaccag cgacttatct gacggcctta agccagccga
     9001 aaatctatcg cctgaacacg tggattgcgg atgcgccaat cgcgctacgt cagccgaatg
     9061 gccaggtctg gtcaccgcag aacgatgacc gtcgttatag cgaaagcggc agagtgatgc
     9121 tggtagatgc gttgacccgt tcgatgaacg tgccgacggt gaatctgggc atggcgttgg
     9181 ggctgcctgc ggttacggag acctggatta aactgggtgt gccgaaagat caactgaatc
     9241 cagttccagc aatgttgttg ggggcgttga acttaacgcc aatcgaagtg gcgcaggcgt
     9301 tccagaccat cgccagcggt ggtaaccgtg ctccgctttc tgcactgcgt tcggtaatcg
     9361 cggaagatgg caaggtgctg tatcagagct tcccgcaggc ggaacgcgct gttccggcgc
     9421 aggcggcgta tctgacacta tggaccatgc agcaggtggt acaacgtggt acggggcgtc
     9481 agcttggggc gaaatacccg aacctgcatc tggcagggaa aacagggact accaacaata
     9541 acgtagatac ctggtttgcg ggcattgacg gcagtacggt gaccatcacc tgggtaggcc
     9601 gtgataacaa ccaaccgacc aaattgtatg gagccagcgg ggcaatgtcg atttatcagc
     9661 gttatctggc taaccagacg ccaacaccgc tgaatcttgt tccgccagaa gatattgcag
     9721 atatgggcgt ggactatgac ggcaactttg tttgcagcgg tggcatgcgt gtcttgccgg
     9781 tctggaccag cgatccgcaa tcgctgtgcc agcagagtga gatgcagcag cagccgtcag
     9841 gcaatccgtt tgatcagtct tctcagccgc agcaacagcc gcaacagcaa cctgctcagc
     9901 aagagcagaa agacagcgac ggtgtagccg gttggatcaa ggatatgttt ggtagtaatt
     9961 aacgtctaag cgtgacatac cggatggcga gtagccatcc ggtaaaataa catcccaccc
    10021 aaagtattaa ccctttcttt tcatctggtt gtttattaac ccttcaggaa agctcagatt
    10081 gcgtaccgct tgcgaacccg ccagcgtttc gaatattatc ttatctttat aataatcatt
    10141 ctcgtttacg ttatcattca ctttatatca gagatatacc aatggcgcgt tccaaaactg
    10201 ctcagccaaa acactcactg cgtaaaatcg cagttgtagt agccacagcg gttagcggca
    10261 tgtctgttta tgcacaggca gcggttgaac cgaaagaaga cactatcacc gttaccgctg
    10321 cacctgcgcc gcaagaaagc gcatggggac cggctgcaac tattgcggcg aagcactctg
    10381 ctactgcgac taaaacggat acaccaattg aaaaaacgcc gcagtctatt tcggttgtga
    10441 caaatgaaga gatgcagatg catcaatttc agtctgtaaa agaagcatta ggttatacac
    10501 ctggcgttac ggttagcagt cgtggtgctt ctaatacata tgattttgtt attattcgtg
    10561 gtttctcatc tgttggtctg aatcaaaata attaccttga tggactaaaa cttcagggca
    10621 acttttataa cgatgctgtg attgatcctt acatgctcga gagggttgaa ctgatgcgcg
    10681 ggccgacgtc tgttctctat gggaaaagta atcctggtgg gattatttct atggtgagta
    10741 agcgtccgac aactgaaccc ctgaaagaaa ttcagtttaa aatggggacg gataatctgt
    10801 ttcagaccgg attcgatttc agtgatgcgc tggatgataa cggtgagttc tcctaccgtt
    10861 tgactggcct cgcacgctca acaaacgaac agcagaaaaa ctctgaatct cagcgttata
    10921 ccattgctcc atcattctca tggcgtccag acgacaaaac caattttact ttcctgtcct
    10981 attttcagaa cgaaccagaa acgggttatt acggttggtt gccgaaagag gggaccgttg
    11041 agccattgcc taatggtaag cgtctaccga ctgacttcaa tgaaggtgca tcgaataata
    11101 catactcccg taaccagaaa atggtgggat atagttttga acatggtttt aatgacacct
    11161 tcaccgtgcg tcagaatctg cgtttcagtg aaatgaaaac ctcacagaaa agtgtttatg
    11221 gcacagggat tgccagcgat ggtcataccc taaaccgcgg gacagtggtg gataatgagc
    11281 gtctgcaaaa ctttagcgtt gatacccaac ttgaaagtaa atttgctaca ggtgaagtcg
    11341 agcatacttt gctgacaggg gtagacttca tgcgtatgcg caatgatatc aatgccagct
    11401 ttggatctgc accatccatc gatctttata acaaatatca tcctgaatac tttgcatttg
    11461 gtaacgcaga gccataccaa atgaatgaaa gcaaacaaac aggtatttat gttcaggatc
    11521 aggcggaatg gaataaatgg gtattcactc tggggggacg ctacgattgg tctaagcaag
    11581 cgaccactgt tcgtgaaaac tcttatacgc cgactgaagg ttatattgag cgtaatgatc
    11641 atcagttcac ctggcgcggt ggtgtaaact acttattcga taatggtatt tcaccttact
    11701 ttagttatag ccagtccttt gaaccgagtg ctttcgatct gtggagcaac ccgcgcgttt
    11761 cctataagcc atcgaaaggt gaacagtatg aagctggcgt aaaatatgtt ccgaatgata
    11821 tgccggtcgt tgttacgggc gcagtctatc aattgacgaa aacaaataac ctgacagcag
    11881 acccaacaaa cccgttagcg caagtcccag caggtgagat tcgcgctcgt ggtgtggaac
    11941 ttgaagcaaa agcggcgtta aatgccaata ttaacttgac ggcttcttat acctacacag
    12001 atgcggaata caccaaagac accaatctca aaggtaaaac tccagaacaa gtaccggagc
    12061 atatggcatc tctctggggg gattatacct tcaatgaagg gccgctttct ggtttaacat
    12121 tgggaacagg tggtcgtttt attggttcca gctatggtga tccggcgaac acttttaaag
    12181 tgggtagcgc agctgtaatg gatgctgttg taaaatatga tctggcacgc tttggtatgg
    12241 cgggatccag ccttgctgtg aacgtcaaca atttgctcga tcgtgagtat gttgccagtt
    12301 gcttccagac ctatggctgc ttctggggcg cagaacgtca ggtcgttgca accgcaacct
    12361 tccgtttcta attttttctt tggggcacgg atttccgtgc ccgcttcaca agttggctgt
    12421 tatgcaggaa tacacgaatc attccgatac cacttttgca ctgcgtaata tctcctttcg
    12481 tgtgccaggg cgcacgcttt tgcatccgct gtcgttaacc tttcctgccg ggaaagtgac
    12541 tggtttgatt ggtcacaacg gttctggtaa atccactctg ctcaaaatgc ttggccgtca
    12601 tcagccaccg tcggaaggcg aaattcttct tgatgcccag ccgctggaaa gctggagcag
    12661 caaagcgttc gcccgcaaag tggcttattt gccgcagcag cttcctccgg cagaagggat
    12721 gaccgtgcgt gaactggtgg cgattggtcg ttacccgtgg catggcgcac tggggcgctt
    12781 tggtgcggca gatcgcgaaa aggtcgaaga ggcgatttcg ctggttggct taaaaccgct
    12841 ggcgcatcgg ctggtcgata gtctctctgg cggcgaacgt cagcgggcgt ggatcgctat
    12901 gctggtggcg caggatagcc gttgtctgtt gctcgacgaa ccgacctcgg cgctggatat
    12961 cgcccaccag gttgatgtgc tggcgctggt gcatcgttta agtcaggaac gtggtctgac
    13021 ggtcattgcc gtgctgcacg atattaatat ggctgcgcgc tactgtgatt atctggtcgc
    13081 cctgcgcggt ggtgaaatga ttgctcaggg aacgcctgcg gaaattatgc gcggcgaaac
    13141 cctcgaaatg atttatggca tcccgatggg tattctgccg catccggcgg gtgctgcacc
    13201 tgtgagtttt gtttattgat gagcggctta cctcttattt cgcgccgccg actgttaacg
    13261 gcgatggcgc tttctccgtt gttatggcag atgaataccg cccacgcggc gattattgat
    13321 cccaatcgta ttgtggcgct ggagtggttg ccggtggaat tactgctggc gctcggcatc
    13381 gtgccttacg gcgtggcgga caccatcaac tatcgcctgt gggtcagcga accgccattg
    13441 ccggactcag tgattgacgt tggtttgcgt acagaaccca accttgaact gctgaccgaa
    13501 atgaaaccgt cgtttatgat ctggtcggca ggatatggcc cttcaccaga aatgctggca
    13561 cgtattgcgc cggggcgcgg atttaacttc agcgacggca aacagccgct ggcgatggcg
    13621 cgtaaatcac tgacggaaat ggcagattta cttaacctgc aaagcgcagc agaaacgcat
    13681 ttagcgcaat atgaagactt tatccgcagc atgaaacccc gctttgtgaa gcgtggtgca
    13741 cgcccgttat tgctgacgac gcttatcgat ccgcgccata tgttggtctt cggtccaaac
    13801 agcttgttcc aggaaattct tgatgagtac ggcatcccaa atgcctggca aggggaaacc
    13861 aacttctggg gcagtaccgc cgtcagtatc gatcgtctgg cggcgtataa agacgttgat
    13921 gtgctctgtt ttgatcacga caacagcaaa gacatggatg cgctaatggc aaccccgctg
    13981 tggcaggcca tgccgtttgt ccgtgcagga cgttttcagc gcgttccggc ggtctggttc
    14041 tatggcgcga cgctttcggc aatgcacttt gtgcgtattc tggataacgc catcggaggt
    14101 aaagcgtgag taaacgaatt gcgctttttc cggcgttatt gctggcgctg ctggtgattg
    14161 tcgctacggc gctcacctgg atgaacttct cgcaggcgtt gccgcgcagc cagtgggcgc
    14221 aggccgcctg gtcgccggat attgacgtca tcgagcagat gatttttcac tacagcttgt
    14281 tgccgcgtct ggcgatttcg ctgctggtgg gcgcgggcct ggggctggtg ggcgtgctgt
    14341 ttcagcaagt gctgcgtaac ccgctggcgg aaccgacgac gttgggcgtt gcaacaggcg
    14401 cgcaactggg gattaccgtc actacgctct gggcgatccc aggtgctatg gcgagccagt
    14461 ttgctgcgct ggcaggggct tgcgttgttg gcttaattgt ctttggcgtc gcgtggggga
    14521 aacggctgtc gccggtaacg ctgatcctcg cggggctggt agtgagcctt tattgtgggg
    14581 caatcaatca gttactggtt atcttccatc acgaccagct acaaagcatg ttcctgtgga
    14641 gcaccggaac gctgacgcaa accgactggg gcggcgttga gcgtttatgg ccgcagctgc
    14701 tgggcggcgt gatgctgacg ttattgctac ttcgcccgtt aaccctgatg gggcttgatg
    14761 atggcgtggc gcgcaatctc ggtctggcct tgtcgctggc gcgtctggcg gcgctgtcgc
    14821 tggcgatagt catcagtgcg ctgctggtga atgcagtggg gattatcggc tttatcgggt
    14881 tgttcgcgcc gctgctggca aaaatgctgg gggcgcggcg tctgctgcca cggttaatgc
    14941 tggcgtcgct gattggtgcg ttaattctgt ggctttctga tcaaatcatc ctctggctga
    15001 ctcgcgtgtg gatggaagtt tccaccggtt cggtcactgc gttgatcggt gcgccgctgc
    15061 tactgtggct gctgccgcgt ttacgcagca ttagcgcgcc ggatatgaag gtcaacgatc
    15121 gtgtcgcggc tgaacgccaa catgtgctgg cgtttgccct cgcgggcggc gtgctgctgt
    15181 tgatggctgt ggtagtggcg ctgtcgtttg gtcgtgatgc gcacggctgg acgtgggcga
    15241 gcggggcgtt gctcgaggat ttaatgccct ggcgctggcc gcgaattatg gcggcgctgt
    15301 ttgcgggcgt catgctggcg gtggcgggct gtattattca gcggctgacc ggaaacccga
    15361 tggcaagccc ggaagtgctg gggattagct ccggcgcggc gtttggcgtg gtgttgatgc
    15421 tgtttctggt gccgggtaat gcctttggct ggctgttacc tgcaggcagt ctcggcgcgg
    15481 cggtgacgct gttgatcatt atgatcgccg ccggacgcgg tggattttcc ccacaccgca
    15541 tgttactggc ggggatggcg ttaagtaccg cgttcaccat gcttttgatg atgttgcagg
    15601 caagtggtga cccgcgaatg gcgcaagtgc tgacctggat ctccggttcg acctacaacg
    15661 cgaccgatgc gcaggtctgg cgcacgggaa ttgtgatggt aattttgctg gcgattaacc
    15721 cgctgtgccg ccgctggctg accattttac cgctgggcgg tgataccgca cgagccgtag
    15781 gaatggcgct gacgccgacg cgaattgcgc tgctgctatt agcggcttgc ctgacggcga
    15841 ccgcgacgat gactattgga ccgttgagtt ttgttggttt aatggcaccg catattgcgc
    15901 ggatgatggg ttttcgacgg acgatgccac acatcgtaat ttcggcgctg gtgggtggtt
    15961 tactgctggt gttcgctgac tggtgtgggc ggatggtatt gtttccattc cagatcccgg
    16021 cggggctgct gtcaaccttt atcggcgcgc catattttat ctatttgttg agaaagcaga
    16081 gccgttaata gaatgcctcg cccggtgggc gaggcaaaat catcacaact tcgcaaacac
    16141 ccgacgtgca gcatcgatgg tgttattgat atcttccatg ctgtgcgcca cggacataaa
    16201 gcccgcttca aacgctgacg gtgccagata aacaccttcg tccagcatca tatggaagaa
    16261 acgcttaaag cgttccacgt cacaggccat cacatcctga tagcacgtca cggactcggc
    16321 gtcggtaaag aaaataccga acatgccgcc aacgtggttt accaccagcg gaattccggc
    16381 ttcttctgcc gcttccagca gaccttctgc cagacgtgtt gtcagctcat ccagcgtctc
    16441 gtgaacgccc ggctgtgcga cttcattcag acaggcgaaa ccagctgcca tcgcaatcgg
    16501 gttaccggaa agcgtacccg cctgatagac cggaccagtc ggggccagcg catccattac
    16561 atcacgacga ccaccgaatg cgcctaccgg cattccaccg ccgatgattt tgccgaggca
    16621 ggttaaatct ggcactacgc cgtaataatc ctgtgcgcca gctagcgcta cgcggaaacc
    16681 ggtcatcact tcatcgatga tcagcaacgc gccaaattcg tcacacagcg cacgcagacc
    16741 tggcaggaac tctggcagcg gtggaacgca gttcatattg cctgccaccg gctcgacgat
    16801 aatacaggca atctcatgcg ggtactgttc gaacgcggcg cgtacagagg ccagatcgtt
    16861 ataagtacag gttaaggtat gtttggcgaa atctgccgga acacccggcg agtttggctg
    16921 gcctaacgtg agtgcgccag aaccggcttt caccagcagg cagtcagcgt gaccgtggta
    16981 acaaccttca aatttaatga ttttgtcgcg accggtaaaa ccacgggcca ggcggattgc
    17041 gctcatggtc gcttcagtgc cggagttcac catgcgcacc atatccatgg tcgggaccag
    17101 ttcggtcacc agttgcgcca ttttcacttc catttcggtc ggtgcaccaa agcttaaacc
    17161 acgctcggcg gcttcaatca cggcattgcg gatcgccgga tggttatggc ccagcaccat
    17221 cggcccccag gaaccgacat aatcgatata ggctttgcca tcaacatcgt acagataagc
    17281 gccgtccgct ttttcgataa acagtggagt gccgcccacg ccagtaaagg cgcgaacagg
    17341 ggagttcaca ccgccaggga tcagctcgcg cgctgcgctg taaagatttt cagacttact
    17401 catggagggt tcctgattcg tagaaaaagt gaatggctgc tattctatgt tattcataac
    17461 aagttaaata ctcgtcaaac atcaggctgc ttgtactggt gcaatcctga tttcttagag
    17521 tataaaagtt ttgtgcattt gaaacattac gctttgcaaa ggattttcat ggaacgtgcg
    17581 agtaaaatgc cgtcatctta tttgtatgac caataagtga tcattggatg aaaactgata
    17641 ctccctcttt agaaacaccg caggccgcgc gcctgcgacg cagacaactg attcgccaac
    17701 ttcttgagcg cgataaaacc ccgttagcca ttttgtttat ggcggcagtc gtcggcacgc
    17761 ttgtcgggct ggctgcggtt gcttttgaca aaggtgtcgc ctggttgcag aaccaacgta
    17821 tgggggcact ggtacatact gctgataatt atccgctact gttaaccgtc gcttttctct
    17881 gttcggcggt gctggcgatg ttcggctact ttttggtgcg caaatacgcg ccggaagcag
    17941 gtggttcggg gatcccggaa attgaagggg cgctggaaga tcaacgtccc gttcgctggt
    18001 ggcgtgtgtt gccggtgaaa ttctttggcg ggctgggaac actcggcgga ggcatggtgc
    18061 tgggccgcga agggccaacc gtacagatcg gcggcaatat cggtcgcatg gtgcttgatg
    18121 ttttccgcct gaaaggtgat gaagcgcgcc atacgctgct ggcaaccggt gctgcggcgg
    18181 ggctggctgc ggcctttaac gccccgctgg cgggtatttt gtttattatc gaagagatgc
    18241 gtccgcagtt tcgctatacg ttaatttcga ttaaagcggt atttattggc gtcattatgt
    18301 cgaccattat gtaccggatt tttaatcatg aagttgcgtt gattgacgtc ggtaaacttt
    18361 ctgacgcgcc gcttaatacg ctgtggcttt atctgatcct cggtattatt tttggcattt
    18421 tcggccctat ttttaataaa tgggtgctgg ggatgcagga tttgctgcac cgtgtgcacg
    18481 gcggcaatat taccaaatgg gtactgatgg gcggtgcaat tggcggtctg tgtggattgc
    18541 tggggtttgt ggcaccagca acgtcgggcg gcggttttaa cctgattcct atcgctaccg
    18601 cggggaattt cagcatggga atgctggtgt ttatcttcgt cgcgcgggtc attaccacct
    18661 tactctgctt ctcttccggc gcgccgggcg gtatttttgc cccgatgctg gcgctgggta
    18721 ctgtgctggg aaccgctttc ggaatggttg ccgttgagct gtttccgcaa tatcaccttg
    18781 aggcggggac atttgctatt gccggaatgg gggcattact ggcggcatct attcgcgcgc
    18841 cgttaacggg gatcattctg gttctggaaa tgaccgataa ctaccagctc attttgccaa
    18901 tgattattac cggtcttggc gcaacactat tagcgcaatt taccggcggg aaaccgctat
    18961 actcggcgat tcttgcgcgc acgctggcga aacaggaagc tgagcaactg gcgcgaagca
    19021 aggccgcatc agccagcgag aatacttgaa cgaaatacca gggtattaga taatggcgat
    19081 tattattggg ttagaatttg cccaattgcc gatgtcgttt ggagcaaaat atgagtgatg
    19141 acgtagcact gccgctggag tttaccgacg cagcagccaa caaagttaaa agcctgatcg
    19201 ctgacgaaga taacccgaat ctgaaattac gcgtgtatat taccggtggc ggttgcagcg
    19261 gcttccagta tggtttcacc tttgatgatc aggtgaacga aggcgatatg accatcgaaa
    19321 aacagggcgt tggcctggtg gttgatccga tgagcctgca atatctggtc ggcggttccg
    19381 ttgactatac cgaaggtctg gaaggttctc gtttcatcgt gaccaacccg aacgcgaaaa
    19441 gcacctgcgg ttgcggttct tcctttagca tctaatctgt tgttcatgtg ccggataaac
    19501 tatccggcac atcttctctc ttcaacgccc attctcatcc agcgcaaacg tcggtagctt
    19561 aagatgccaa cgaatagccg ccagccgaat caatagcgtc acgaccatgc ccatcatact
    19621 ggctgtttcc agtggtacgg aaaatgtgta ataagccgta gcgtggacaa taccgccgat
    19681 aatacaggct gttgcgtaga tttctgtacg taaaatcatg gggatctcgc gggcaagtac
    19741 atcacgaatg atcccgccgc caacgccagt aatgacgccc atacaaaccg cgattaacgg
    19801 accggcttcc gcattaaagg ctttattcac gccaatgccg acaaacactg ccagaccaac
    19861 ggcgtccagc accggcaaca tccattttgg taagcgtctt ggctggcgca ccagcacgat
    19921 ggtcagcatg ctggtgacca ttgcaacgac cagatcggtg ggatctttca cccaaaatac
    19981 cgggccgtga tccagcgcca tgtcgcgaat tgttccgccg cctactgcgg taaccacgcc
    20041 caataccaga acaccaaaag ggtccatacg caattttccg gctaacaaaa cgccagagat
    20101 agcaaatacc gctgtgccga ctatatccag ccaatagacg agcattgttc aatccccact
    20161 gagcacctga aaaggtcagg cattaatcta cctgtgaaag cgcattacag agctgttgtg
    20221 cagcgaggat aatacgtggg cttgcacgtt caaaccagtc actcgtgaga ggaataacgg
    20281 gaattttgag ctgttcaccc cagtattgtt tgattttagg aatttggtcc ggtccgcctg
    20341 taatgacaat cgcctgtggc gcgcgtgcta acacctgttc gcggctaact tgcggccagg
    20401 gaacccggct gtctttaaag atgttttctc cgccacaaac ttcgagtacc tggttttgaa
    20461 tcgactcttt tccactagta aatggcggat taatgccgaa ttgcagaaaa acacgttttt
    20521 taggtttatc agcatattgc gctttcaatt gcgcgtactg gtccagcagg gattgcgcgg
    20581 cttgttcggc cttatccggt tgcggactcc aggggaccag ttgacgtaac gcattggcaa
    20641 tttgttcaat gcttgtcgca tcgacccaca tcacttttat tcccagagaa gccagctggt
    20701 caacctgccg ctcggcatta cctccacgcc aggcaatcac cagatcgggt ttcagcgcca
    20761 caatgcgttc cagattcatc ccctgccagg tggaaacctg ctcaattttt tgcgcttgtg
    20821 gaggatagtc ggaatagctg ctgaccccaa ccggcgtgat cccggcggca aaggcaagtt
    20881 cagtgttggc gggagaaagc gtgatgacgc gcggcgcggc gttgagccac agcggcgcga
    20941 gaaaagacag ggcgaccagc gccctgaaca gtgacttagc catgtgccag tttctgcacc
    21001 agtgactcaa ccatcaggct ggactgttta gcggcaacag ccaggaactc atcgaagcta
    21061 agatgagact gttgatcggc cacgtcggaa atggcgcgta cgacaacaaa cgggacgttg
    21121 aaattgtggc agacatgggc gattgccgtc gcttccatct ctacagcaat ggcctgtggg
    21181 aagttgtggc ggattttcgc cagaccaaca gaaccattga tgaaagcgtc gccgctgaca
    21241 atcaggccac gtacagcgtt tagattcagt tcggcaatgc aggcctcagc ggcagcgatc
    21301 agtttatcgt cagctttaaa gcctgccggg cagcctggta actggccgta ttcataacca
    21361 aatgccgtga catccgcgtc gtgataacgc gcttcgtccg agacaacgat atcgcccact
    21421 ttcagtgttg gtgccaggcc accggcagaa ccggtgttaa taatcacatc tggcttgcag
    21481 tgttccaaca gcaaagtggc acccagcgcc gcagcgactt taccgatgcc cgatttcaga
    21541 agcgcaacct cggttccatt cagttggccg gtatagattt cgcaaccgcc gagactgata
    21601 gtttgacggt tttcgatttt gtcacgcagt agcgtaactt cttcttccat tgcaccaatg
    21661 atgccgattt tcatagattt actcgcgata agcccgattt gaaggcatag tttaccatgc
    21721 gcttacgggg aagcgtattt ctcacgcggg agaggacatg gcacagattg atttccgaaa
    21781 aaaaataaac tggcatcgtc gttaccgttc accgcagggc gttaaaaccg aacatgagat
    21841 cctacggatc ttcgagagcg atcgcgggcg tatcatcaac tctccggcaa ttcgtcgtct
    21901 gcaacaaaaa acccaggttt ttccactgga gcgcaatgcc gccgtgcgca cgcgtcttac
    21961 ccactcgatg gaagtccagc aggtggggcg ctacatcgcc aaagaaattt taagccgtct
    22021 gaaagagctt aaattactgg aagcatacgg cctggatgaa ctgaccggcc cctttgaaag
    22081 cattgttgag atgtcatgcc ttatgcacga tatcggcaat ccgccgtttg gtcattttgg
    22141 cgaagcggcg ataaatgact ggtttcgcca gcgtttgcac ccggaagatg ccgaaagcca
    22201 gcctctgact gacgatcgct gcagcgtggc agcactacgt ttacgggacg gggaagaacc
    22261 gcttaacgag ctgcggcgca aaatacgtca ggacttatgt cattttgagg ggaatgcaca
    22321 aggcattcgt ctggtgcata cattgatgcg gatgaatctc acctgggcac aggttggcgg
    22381 tattttaaaa tatactcgtc cggcgtggtg gcgtggtgaa acgcctgaga cacatcacta
    22441 tttaatgaaa aagccgggtt attatctttc tgaggaagcc tatattgccc ggttgcgtaa
    22501 agaacttaat ttggcgcttt acagtcgttt tccattaacg tggattatgg aagctgccga
    22561 cgacatctcc tattgtgtgg cagaccttga agatgcggta gagaaaagaa tatttaccgt
    22621 tgagcagctt tatcatcatt tgcacgaagc gtggggccag catgagaaag gttcgctcta
    22681 ttcgctggtg gttgaaaatg cctgggaaaa atcacgctca aatagtttaa gccgcagtac
    22741 ggaagatcag ttttttatgt atttacgggt aaacacccta aataaactgg taccctacgc
    22801 ggcacaacga tttattgata atctgcctgc gattttcgcc ggaacgttta atcatgcatt
    22861 attggaagat gccagcgaat gcagtgatct tctaaagcta tataaaaatg tcgctgtaaa
    22921 acatgtattt agccatccag atgtcgagca gcttgaattg cagggctatc gggtcattag
    22981 cggattatta gagatttatc gccctttatt aagcctgtcg ttatcagact ttactgaact
    23041 ggtagaaaaa gaacgggtga aacgtttccc tattgaatcg cgcttattca acaaactctc
    23101 gacgcgccat cggctggcct atgtcgaggc tgtcagtaaa ttaccgtcag attctcctga
    23161 gtttccgcta tgggaatatt attaccgttg ccgcctgctg caggattata tcagcggtat
    23221 gaccgacctc tatgcgtggg atgaataccg acgtctgatg gccgtagaac aataaccagg
    23281 cttttgtaaa gacgaacaat aaatttttac cttttgcaga aactttagtt cggaacttcg
    23341 cgctataaaa cgaatctgaa gaacacagca attttgcgtt atctgttaat cgagactgaa
    23401 atacatgaaa aaaaccacat tagcactgag tgcactggct ctgagtttag gtttggcgtt
    23461 atctccgctc tctgcaacgg cggctgagac ttcttcagca acgacagccc agcagatgcc
    23521 aagccttgca ccgatgctcg aaaaggtgat gccttcagtg gtcagcatta acgtagaagg
    23581 tagcacaacc gttaatacgc cgcgtatgcc gcgtaatttc cagcagttct tcggtgatga
    23641 ttctccgttc tgccaggaag gttctccgtt ccagagctct ccgttctgcc aggggggcca
    23701 gggcggtaat ggtggcggcc agcaacagaa attcatggcg ctgggttccg gcgtcatcat
    23761 tgatgccgat aaaggctatg tcgtcaccaa caaccacgtt gttgataacg cgacggtgat
    23821 taaagtccag ctgagcgatg gccgtaagtt cgacgcgaag atggttggca aagatccgcg
    23881 ctctgatatc gcgctgatcc agatccagaa cccgaaaaat ctgaccgcaa ttaagatggc
    23941 ggattctgat gcactgcgcg tgggtgatta caccgtagca attggtaacc cgtttggtct
    24001 gggcgagacg gtaacttccg ggattgtctc tgcgctgggg cgtagcggcc tgaatgctga
    24061 aaactacgaa aacttcatcc agaccgatgc agcgatcaac cgtggtaact ccggtggtgc
    24121 gttggttaac ctgaacggcg aactgatcgg tatcaacacc gcgatcctcg caccggacgg
    24181 cggcaacatc ggtatcggtt ttgctatccc gagtaacatg gtgaaaaacc tgacctcgca
    24241 gatggtggaa tacggccagg tgaaacgcgg tgagctgggt attatgggga ctgagctgaa
    24301 ctccgaactg gcgaaagcga tgaaagttga cgcccagcgc ggtgctttcg taagccaggt
    24361 tctgcctaac tcctccgctg caaaagcggg cattaaagcg ggtgatgtga tcacctcact
    24421 gaacggtaag ccaatcagca gctttgccgc cctgcgtgct caggtgggta ctatgccggt
    24481 gggcagcaaa ctgaccctgg gcttactgcg cgacggtaag caggttaacg tgaacctgga
    24541 actgcagcag agcagccaga atcaggttga ttccagctcc atcttcaacg gcattgaagg
    24601 cgctgagatg agcaacaaag gcaaagatca gggcgtggta gtgaacaacg tgaaaacggg
    24661 cactccggct gcgcagatcg gcctgaaaaa aggtgatgtg attattggcg cgaaccagca
    24721 ggcagtgaaa aacatcgctg aactgcgtaa agttctcgac agcaaaccgt ctgtgctggc
    24781 actgaacatt cagcgcggcg acagcaccat ctacctgtta atgcagtaat ctccctcaac
    24841 cccttcctga atacgggaag gggttctcct tacaatctgt gaacttcgcc acaactccat
    24901 acatcttcat catcctttgg gcatttgcac aatgccgtac gttacgtact tccttatgct
    24961 aagccgtgca taacggagga cttatggctg gctggcatct tgataccaaa atggcgcagg
    25021 atatcgtagc acgtaccatg cgcatcatcg ataccaatat caacgtaatg gatgcccgcg
    25081 ggcgaattat cggcagcggc gatcgtgagc gtattggtga attgcacgaa ggagcattgc
    25141 tggtactttc acagggacga gtcgtcgata tcgatgacgc ggtggcacgt catctgcacg
    25201 gtgtgcggca ggggattaat ctaccgttac ggctggaagg tgaaattgtc ggcgtaattg
    25261 gcctgacagg tgaaccagag aatctgcgta aatatggcga actggtctgc atgacggctg
    25321 aaatgatgct ggaacagtcg cggttgatgc acctgttggc gcaggatagc cgtttgcggg
    25381 aagaactggt gatgaacctc attcaggctg aggagaatac tcccgcactt actgaatggg
    25441 cgcaacggct ggggatcgat ctcaatcaac cgcgagtggt ggctattgtt gaggtcgaca
    25501 gcggtcagct tggcgtggac agcgcaatgg cggagttaca gcaactgcaa aatgcgctga
    25561 ctacgcccga gcgtaataat ctggtggcga ttgtctcgct aaccgaaatg gtggtgttga
    25621 aaccggcgtt gaactctttt gggcgctggg acgcagaaga tcatcgtaag agagttgaac
    25681 aactgattac ccgcatgaaa gagtatggcc agttgcgttt tcgcgtttcg ctgggcaact
    25741 attttaccgg tcctggcagt attgcccgat cctatcgtac ggcgaaaacg acgatggtag
    25801 tggggaaaca gcggatgcca gaaagtcgct gctattttta tcaggatctg atgttacctg
    25861 tgttactcga cagtttgcgt ggcgactggc aggccaacga actggcgcga ccgctggcgc
    25921 ggctgaaagc gatggataat aacggcttgc tgcgacgtac gctggcggcg tggtttcgcc
    25981 ataacgtgca gccgctggca acgtcaaagg cgctgtttat tcatcgtaat accctggagt
    26041 atcggcttaa tcgtatatcg gaactcacag gcttagatct ggggaatttt gatgacaggt
    26101 tattgttgta tgtggcgtta caactggatg aacagcgata gggtaaaaat agtaaatgcc
    26161 gggaaatatg ctcgtcttaa ttcaagttgc atgtacggcg actcgaatta ttcagcgtat
    26221 actttacccg gctggcagaa tggcaatatg caaaattatt tacgtgtcag cttttctaaa
    26281 tctgcttcta tctcgctgat tttattggtc accactgact ccaggtgacg caagtcgtcg
    26341 agaattttac gcttaaggtc aacttcgcta cgatcacgct ggcagatttg atcaagctca
    26401 tcaatgatat aacgaagatt agggctgatt tcctgaactt ctttatatcc ctggccaata
    26461 ccatcggcga ccaccgtttt gcgctgacgc ggatatttaa acttcacact cttggcgaaa
    26521 aactcgcctt tatctttctg aaaatagatt ttcaaaatat cgttattggc ttcctgccgg
    26581 aggctgtaac gatcaatttc ctcaggattg gtaatgccca aacttttcag attgtcgtac
    26641 atagttgtac ctcaaaagag tcagtaattg tgcttatctt agcatttcgc ctggcggttt
    26701 agctcgacgg agatcgcagg aagccttttc ttccagactt ttatcatggc ctaaaaatca
    26761 aaaaattacc tgctttattc tgataagaga attcgctttc tacaccagtt ccaggattgt
    26821 cacttccggg cggcaattca gacgcaagcc atacaaactt ccgacacccc gagtcgtgta
    26881 aatttgtcgc tcaccaaagg catttaaacc ggcggcgtag cgtttatctt ctatgggcgc
    26941 aaaaggctca ccaaccaacg gtatgcgtaa ctgcccgccg tgggtgtgac cacagagcat
    27001 cagatcccac ttttcattac gcatgacttc tttgctgtcg ggattatgcg ccagcaccag
    27061 tcgtggcaga tttgcttcgc tggcgggagg cggtttgcat tgtccggtcc ataaatcacc
    27121 cgtgccaacc agttcaaatt gctgcttttg agtggtgatt agcatggctt cgttaaataa
    27181 caccgtgatc cccgccgatt ttaatgcctc gccaattaag cgatttttct ctgtaccaac
    27241 agggcgatcg tggttgccgt aacaggcaaa tgtcggcgca cattcggcaa gggaggagag
    27301 tacgtcacta aacgccgaaa aattcagctg catatcaaat aatacgtaat cgccgcccag
    27361 caatatcaaa tcgggctttt gtgctacgcc aagagcaatt gcgtcagaaa tcaggcttaa
    27421 aggaacaaaa cgagagtaat ggagatctgc cagaaaaagg attttgaaag gagcagcgtt
    27481 gtctttaaaa aaggcgaggc ggtgacggat taattcaagc cagccaggtt cccaataatg
    27541 catatagctg aggcttgatc cggcagctaa cgtcgcagcg gctgcctgta aaaaacgcct
    27601 gcgtgtgatc atcatccatc cctgcaaaaa atcgggcagc gtcatgctgc ccgtgtactg
    27661 atttttagtc gatggtacgc agcagttcgt taatgccgac tttgccgcga gttttcgcgt
    27721 caactttctt aacgataact gcacaataaa ggctgtattt gccatctttc gacggcaggt
    27781 tgccagaaac aaccaccgaa ccagccggaa cgcgaccgta gtggatttcg ccggtttcac
    27841 ggtcgtaaat acgggtgctc tgaccaatgt atacgcccat agagatgacg gaaccttctt
    27901 cgacaatcac cccttcaacc acttcagagc gtgcaccgat gaagcaatta tcttcaataa
    27961 tggtcgggtt agcctgcagc ggctccagca cgccgccgat gcctacaccg ccagacagat
    28021 ggacgttttt accaatttgt gcacaggaac cgacagtggc ccaggtatca accatagtgc
    28081 cttcatcaac atatgcgccg atgttgacgt aagacggcat cagcacggtg ttacgggcaa
    28141 taaatgcacc ctgacgtacc gccgctggtg gcacaacgcg gaagccttct ttctgaaaac
    28201 gcgcttcgtc gtagtcggcg aatttcatcg gcactttatc gaagtagcgg ctttctgccc
    28261 cttcgatcac ctgattatca ttaatacgga aagagagcag caccgctttt ttcaaccact
    28321 gatgcgtcac ccactgaccg tcaatttttt ccgctacacg cagtgcgccg gaatccagca
    28381 gggcgatcac ctgatttacc gcttcgcggg ttacggtgtc tgcattggct ggcgtgatct
    28441 cggcacggcg ttcaaaagcg gtttcaataa tgttctgtaa ctgctgcatt gttaaactct
    28501 tttcatatca gtaaacacat caccctttat cgtttggatt gagggcctct gtcaaccgct
    28561 gatgcacttc ctgctgcaac tcgttattaa gcgcacgccg gtcagcggtg gcaattatga
    28621 ataaatcttc tactcgctcg ccaatggttg taattcgggc gccatgaagc gaaattccca
    28681 gatcggcaaa aattttcccg actcgcgcca gcagtccagg ttggtcgagg gcgatcagtt
    28741 cgaggaacga tttgcggtcg gtatgggtcg gcaaaaacgt cacttcggtt tcaacagtaa
    28801 aatggcgcaa tttggcgggt tggcgacggg gctgcggtgg ctgccagcta ctttgcgtca
    28861 gcacctgctc cagaccaaac cgaataacct catgacgatc tgcggatagc gggctaccat
    28921 cgggttccag cacgatgaag gtatccatcg ccataccgtc gcgagtggtg aaaatttgtg
    28981 cgtcgtgaac acttaaattg cggcggtcta attcggcaca gacggcggca aacagatagg
    29041 ggcggtccgg gctccagata aaaatctcgg tgcctccacg cgtagcctgc ggactaagca
    29101 ataccagcgg tttgcttaaa tcatgctgta ataaatggcg cgcatgccag gccagttgat
    29161 ttgggctatg gcggacaaaa tagttagcac gacagcgtga ccaaatttgg tgcagcgcct
    29221 cttcgtcgat gttatccatg cgcagtagtg ccagcgcctg gagttggtga tggcgaaccc
    29281 gttcgcgcat atccggcgtg ttttgcattc cgcgtcgtag ctgcttttcg gtggcgaaat
    29341 agagttcgcg caacagactt tgcttccagc tattccacag cgtttcgttg gtggcgcata
    29401 tatcggccac tgtcaggcat accagataac gaagtcggtt ttctgtttgc acttcttcgg
    29461 caaactgctt gatcacttcc gggtcctgaa tatcgcggcg ttgggcggtc accgacatca
    29521 gcaagtgctg gcgaaccagc cacgccacca gctgtgtttc gcgcgagtta agcccgtgga
    29581 gttcggcaaa atgcaccacg tcttgtgcac cgagaatgga gtggtcgccg ccacgccctt
    29641 tggcgatatc gtgaaacagc gcggcgatga aaatcagctc agttgacggc aggcgcggcc
    29701 agacgtctac acacaacgga tggcgctggc gcgtttcttc actggcaaaa ctctccagtt
    29761 tcagcatcac gcgaatagta tgttcatcca ccgtgtaggc gtggaacagg tcaaactgca
    29821 tctgcccgac gatatgcgac cattggggca tatacgcgcc gagcacgcta tgacgatgca
    29881 tcggtaatag tccgcgacgc accgcaccag ggtgacgcag aatgctcaaa aacagttttc
    29941 gcgcttccgg aatattacac agcggttgtt gcaaatggcg acgggcgtgg cgtaactggc
    30001 gcagcgtggt agagtaaatg ccggtgatcg cactgttgcg caccatggtg tagaacatac
    30061 gcaagatggc ttccggctgg cgcataaata gtgtttcatc acgcaggtcg atcagcgttc
    30121 cgcgtagctg aaactcatcg tcgattggac gtggtttttc gtcggcggga agggcgagga
    30181 tggcttcatc gaacagttgc agcagcatct ggttgagttc actgacgcgg cgtgtaacgc
    30241 ggaagtaatc cttcatcatc cgctcgaccg gttcgttacc ttcgccactg taattcagac
    30301 gctgggcgac gctaagctgg cgatcaaaca gcaggcgatt atcgtaacgg ctgacgacca
    30361 gatgcagggc aaagcgaata cgccacaata tatgcagaca ttcgtttaat tccgctcgct
    30421 ccgctgaggt taagaagcca aacccgacca tttcatccag cgatgttgcg ccaaaatgac
    30481 ggcgggccac ccattgcaga gtgtgaatat cgcgcaagcc gccaggacta cttttgatgt
    30541 ctggttcaag gttgtagctg gtgccgtggt aacgctgatg gcgctggttc tgttcttcaa
    30601 ctttcgctgc gtagaacttg tcggaaggcc agaatccttc gctaaaaata tgtttttgca
    30661 gttcgaggaa gagcgctaca tcgccaatta ataagcggga ttcgattaaa ttggtggcga
    30721 cggttaaatc cgataaccct tccagcatgc actcttcaag cgtgcgcacg ctatgaccga
    30781 cttccagctt tacatcccag agcagcgtta acagctcgcc cactttttgc gcctgatcat
    30841 ccgggagctt tttacggctt aaaatcagta aatcgatgtc ggaaagtgga tgcagctcgc
    30901 cacgaccata accaccgacg gcaaccaatg ccaggtcggc aatctggctg aatcccgctt
    30961 caatccataa tcgttgcagg agttggtcga taaactcggt gcgtgcctca atcaactgtt
    31021 ctgcggagat cccattgtca aaggcatcac ccagccaacg ctggaaagta tcgatatggg
    31081 cttttatccc acagacggtt aattcatcac ggggccaggc gcatggattt tgcggttgac
    31141 cgggcagggt ggggagagcg gtgtttgcgt actgttctgg aagggtattc attgtgcgcc
    31201 acccataaga ttaaattatc gcattaaaaa agccggcatt cgccggcttc atcttattcg
    31261 tcgtgcgaga ttatcgccgg gatggtgtca tccttgcgta gcgtcagaat ttcgcagccg
    31321 ttatcagtca ccacaatagt atgctcatat tgtgcagaca agctgcgatc tttggttttt
    31381 accgtccagc catctttcat ggtgcggatc tcttttttac ccgcgttgac cattggctcg
    31441 atggtgaacg tcatcccagg tttcagtacg acgttggttt cacgggagtc atagtgcagc
    31501 acctgcggtt cttcgtggaa gccgcgacca ataccgtgtc cacaatattc acgaacgacg
    31561 gagaagcctt ctgcttcgac aaatttctga attgccgcac cgatttcgcg cagattaatg
    31621 ccaggtttta ccatgcgtag cgccaggtac aggctttctt gcgtgatgcg gcacagacgt
    31681 tcgcccatga tggtcggctt accgacgata aacattttcg aggtatcgcc gtggaaacca
    31741 tctttgatta cggtgacatc aatgttaacg atatcgccat ctttcagcag cttggcgtcg
    31801 tccgggatac cgtggcacac cacttcatta atagaaatgc aaacggattt cggatagccg
    31861 tgatagccga ggcaggcaga aaccgcgtgt tgttcattaa caatgtaatc attacagatg
    31921 cgatccagct cgccggtgct gacgcccggt ttaacatacg gttcgatcat ctccagcact
    31981 tcggcagcca gtcggccagc gacgcgcatt ttttcgatat cttctggggt cttgattgag
    32041 atagccatta attctgtcca tcagcgtcgg tgataccgac aatatatatg taagtgccgt
    32101 caatggtatc acacccgggc aaattgagaa tcattctgaa tttcgccaaa cgtgccactg
    32161 aaggttttct ataatagaaa attcaacgtc tgatgctgta cacagcgcca acaattattg
    32221 gtgtccacga cgtatttgtg gtataaagcg cgccggactt ccgatccatt tcgtatacac
    32281 agactggacg gaagcgacaa tctcactttg tgtaacaaca cacacgtatc ggcacatatt
    32341 ccggggtgcc ctttggggtc ggtaatatgg gatacgtgga ggcataaccc caacttttat
    32401 atagaggttt taatcatggc aactgtttcc atgcgcgaca tgctcaaggc tggtgttcac
    32461 ttcggtcacc agacccgtta ctggaacccg aaaatgaagc cgttcatctt cggtgcgcgt
    32521 aacaaagttc acatcatcaa ccttgagaaa actgtaccga tgttcaacga agctctggct
    32581 gaactgaaca agattgcttc tcgcaaaggt aaaatccttt tcgttggtac taaacgcgct
    32641 gcaagcgaag cggtgaaaga cgctgctctg agctgcgacc agttcttcgt gaaccatcgc
    32701 tggctgggcg gtatgctgac taactggaaa accgttcgtc agtccatcaa acgtctgaaa
    32761 gacctggaaa ctcagtctca ggacggtact ttcgacaagc tgaccaagaa agaagcgctg
    32821 atgcgcactc gtgagctgga gaaactggaa aacagcctgg gcggtatcaa agacatgggc
    32881 ggtctgccgg acgctctgtt tgtaatcgat gctgaccacg aacacattgc tatcaaagaa
    32941 gcaaacaacc tgggtattcc ggtatttgct atcgttgata ccaactctga tccggacggt
    33001 gttgacttcg ttatcccggg taacgacgac gcaatccgtg ctgtgaccct gtacctgggc
    33061 gctgttgctg caaccgtacg tgaaggccgt tctcaggatc tggcttccca ggcggaagaa
    33121 agcttcgtag aagctgagta ataaggcttg ataactcccc caaaatagtt cgagttgcag
    33181 aaaggcggca agctcgagaa ttcccgggag cttacatcag taagtgaccg ggatgagcga
    33241 gcgaagataa cgcatctgcg gcgcgaaata tgaaggggga gagcccttat agaccaggta
    33301 gtacacgttt ggttaggggg cctgcatatg gccccctttt tcacttttat atctgtgcgg
    33361 tttaatgccg ggcagatcac atctccgagg attttagaat ggctgaaatt accgcatccc
    33421 tggtaaaaga gctgcgtgag cgtactggcg caggcatgat ggattgcaaa aaagcactga
    33481 ctgaagctaa cggcgacatc gagctggcaa tcgaaaacat gcgtaagtcc ggtgctatta
    33541 aagcagcgaa aaaagcaggc aacgttgctg ctgacggcgt gatcaaaacc aaaatcgacg
    33601 gcaactacgg catcattctg gaagttaact gccagactga cttcgttgca aaagacgctg
    33661 gtttccaggc gttcgcagac aaagttctgg acgcagctgt tgctggcaaa atcactgacg
    33721 ttgaagtact gaaagcacag ttcgaagaag aacgtgttgc gctggtagcg aaaattggtg
    33781 aaaacatcaa cattcgccgc gttgctgcgc tggaaggcga cgttctgggt tcttatcagc
    33841 acggtgcgcg tatcggtgtt ctggttgctg ctaaaggcgc tgacgaagag ctggttaaac
    33901 acatcgctat gcacgttgct gcaagcaagc cagaattcat caaaccagaa gacgtatccg
    33961 ctgaagtggt agaaaaagaa taccaggtac agctggatat cgcgatgcag tctggtaagc
    34021 cgaaagaaat cgcagagaaa atggttgaag gccgcatgaa gaaattcacc ggcgaagttt
    34081 ctctgaccgg tcagccgttc gttatggaac caagcaaaac tgttggtcag ctgctgaaag
    34141 agcataacgc tgaagtgact ggcttcatcc gcttcgaagt gggtgaaggc atcgagaaag
    34201 ttgagactga ctttgcagca gaagttgctg cgatgtccaa gcagtcttaa ttatcgaaaa
    34261 ggagccgcct gagggcggct tctttttgtg cccatcttgt aaattcagct aacccttgtg
    34321 gggctgcgct gaaaagcgac gtacaatgtc gctagtatta attcatttca atcgttgaca
    34381 gtctcaggaa agaaacatgg ctaccaatgc aaaacccgtc tataaacgca ttctgcttaa
    34441 gttgagtggc gaagctctgc agggcactga aggcttcggt attgatgcaa gcatactgga
    34501 tcgcatggct caggaaatca aagaactggt tgaactgggt attcaggttg gtgtggtgat
    34561 tggtgggggt aacctgttcc gtggcgctgg tctggcgaaa gcgggtatga accgcgttgt
    34621 gggcgaccac atggggatgc tggcgaccgt aatgaacggc ctggcaatgc gtgatgcact
    34681 gcaccgcgcc tatgtgaacg ctcgtctgat gtccgctatt ccattgaatg gcgtgtgcga
    34741 cagctacagc tgggcagaag ctatcagcct gttgcgcaac aaccgtgtgg tgatcctctc
    34801 cgccggtaca ggtaacccgt tctttaccac cgactcagca gcttgcctgc gtggtatcga
    34861 aattgaagcc gatgtggtgc tgaaagcaac caaagttgac ggcgtgttta ctgctgatcc
    34921 ggcgaaagat ccaaccgcaa ccatgtacga gcaactgact tacagcgaag tgctggaaaa
    34981 agagctgaaa gtcatggacc tggcggcctt cacgcttgct cgtgaccata aattaccgat
    35041 tcgtgttttc aacatgaaca aaccgggtgc gctgcgccgt gtggtaatgg gtgaaaaaga
    35101 agggacttta atcacggaat aattcccgtg atggataaat aagggtaaga ttccgcgtaa
    35161 gtatcgcggg ggcgtaagtc tggttataag gcgttattgt tgcaggcagt ttggtcacgg
    35221 ccagcgcgca gcaaccggag cgtacaaaag tacgtgagga tggcgagcac tgcccggggc
    35281 caaaatggca aataaaatag cctaataatc cagacgatta cccgtaatat gtttaatcag
    35341 ggctatactt agcacacttc cactgtgtgt gactgtctgg tctgactgag acaagttttc
    35401 aaggattcgt aacgtgatta gcgatatcag aaaagatgct gaagtacgca tggataaatg
    35461 cgtagaagcg ttcaaaaccc aaatcagcaa aatacgcacg ggtcgtgctt ctcccagcct
    35521 gctggatggc attgtcgtgg aatattacgg cacgccgacg ccgctgcgtc agctggcaag
    35581 cgtaacggta gaagattccc gtactctgaa aatcaacgtg tttgatcgtt caatgtctcc
    35641 ggccgttgaa aaagcgatta tggcgtccga tcttggcctg aacccgaact ctgcgggtag
    35701 cgacatccgt gttccgctgc cgccgctgac ggaagaacgt cgtaaagatc tgaccaaaat
    35761 cgttcgtggt gaagcagaac aagcgcgtgt tgcagtacgt aatgtgcgtc gtgacgcgaa
    35821 cgacaaagtg aaagcactgt tgaaagataa agagatcagc gaagacgacg atcgccgttc
    35881 tcaggacgat gtacagaaac tgactgatgc tgcaatcaag aaaattgaag cggcgctggc
    35941 agacaaagaa gcagaactga tgcagttctg atttcttgaa cgacaaaaac gccgctcagt
    36001 agatccttgc ggatcggctg gcggcgtttt gctttttatt ctgtctcaac tctggatgtt
    36061 tcatgaagca actcaccatt ctgggctcga ccggctcgat tggttgcagc acgctggacg
    36121 tggtgcgcca taatcccgaa cacttccgcg tagttgcgct ggtggcaggc aaaaatgtca
    36181 ctcgcatggt agaacagtgc ctggaattct ctccccgcta tgctgtaatg gatgatgaag
    36241 cgagtgcgaa acttcttaaa acgatgctac agcaacaggg tagccgcacc gaagtcttaa
    36301 gtgggcaaca agccgcttgc gatatggcag cgcttgagga tgttgatcag gtgatggcag
    36361 ccattgttgg cgctgctggg ctgttaccta cgcttgctgc gatccgcgcg ggtaaaacca
    36421 ttttgctggc caataaagaa tcactggtta cctgcggacg tctgtttatg gacgccgtaa
    36481 agcagagcaa agcgcaattg ttaccggtcg atagcgaaca taacgccatt tttcagagtt
    36541 taccgcaacc tatccagcat aatctgggat acgctgacct tgagcaaaat ggcgtggtgt
    36601 ccattttact taccgggtct ggtggccctt tccgtgagac gccattgcgc gatttggcaa
    36661 caatgacgcc cgatcaagcc tgccgtcatc cgaactggtc gatgggacgt aaaatttctg
    36721 tcgattcggc taccatgatg aacaaaggtc tggaatacat tgaagcgcgt tggctgttta
    36781 acgccagcgc cagccagatg gaagtgctga ttcacccgca gtcagtgatt cactcaatgg
    36841 tgcgctatca ggacggcagt gttctggcgc agctggggga accggatatg cgtacgccaa
    36901 ttgcccacac catggcatgg ccgaatcgcg tgaactctgg cgtgaagccg ctcgattttt
    36961 gcaaactaag tgcgttgaca tttgccgcac cggattatga tcgttatcca tgcctgaaac
    37021 tggcgatgga ggcgttcgaa caaggccagg cagcgacgac agcattgaat gccgcaaacg
    37081 aaatcaccgt agctgctttt cttgcgcaac aaatccgctt tacggatatc gccgcgttga
    37141 atttatccgt actggaaaaa atggatatgc gcgaaccaca atgtgtggac gatgtgttat
    37201 ctgttgatgc gaacgcgcgt gaagtcgcca gaaaagaggt gatgcgtctc gcaagctgag
    37261 gataatccgg ctacagagag tcgcgctatt tgttagcgta gggcttcagt gatatagtct
    37321 gcgccatctg atcgtaagta gttggcttta taaggtcagg tatgccgtgg ttttacacgg
    37381 cttttttttg tataggcttc agtattcctg agtaccgtaa accctgtcag ggaataagaa
    37441 acgcgtgatg ttgtctgcta ctcaaccact tagcgaaaaa ttgccagcgc atggctgtcg
    37501 tcatgttgcg atcattatgg acggcaatgg tcgctgggca aaaaagcaag ggaagattcg
    37561 tgcctttggg cataaagccg gggcaaaatc cgtccgccgg gctgtctctt ttgcggccaa
    37621 caacggtatt gaggcgttaa cgctgtatgc ctttagtagt gaaaactgga accgaccagc
    37681 gcaggaagtc agtgcgttaa tggaactgtt tgtgtgggcg ctcgatagcg aagtaaaaag
    37741 tctgcaccga cataacgtgc gtctgcgtat tattggcgat accagtcgct ttaactcgcg
    37801 tttgcaagaa cgtattcgta aatctgaagc gctaacagcc gggaataccg gtctgacgct
    37861 gaatattgcg gcgaactacg gtggacgttg ggatatagtc cagggagtca ggcaactggc
    37921 tgaaaaggtg cagcaaggaa acctgcaacc agatcagata gatgaagaga tgctaaacca
    37981 gcatgtctgt atgcatgaac tggcccctgt agatttagta attaggactg ggggggagca
    38041 tcgcattagt aactttttgc tttggcaaat tgcctatgcc gaactttact ttacagatgt
    38101 tctctggccc gatttcgatg aacaagactt tgaaggggcg ttaaatgcct ttgctaatcg
    38161 agagcgtcgt ttcggcggca ccgagcccgg tgatgaaaca gcctgatggg ggtcgctttt
    38221 gctgaagtat cgcctgatat ctgcttttgt gttaataccc gttgtcatcg cggcgttgtt
    38281 tctgttgccg ccggtggggt tcgccattgt aacgctggtg gtctgcatgc tggcagcgtg
    38341 ggaatgggga cagcttagcg gttttaccac tcgttcgcag cgagtatggt tggcggtgtt
    38401 atgcgggtta ttgttggcgc tgatgctttt tctgttgccg gaatatcacc gaaatattca
    38461 tcaaccgctg gttgaaatct cactttgggc ttcgctgggt tggtggattg tcgcgctatt
    38521 gctggtgctg ttttaccctg gttccgcagc aatctggcgt aactctaaaa cattgcgcct
    38581 tatttttggc gtgctaacca ttgttccctt cttctggggc atgctggcgt tacgggcctg
    38641 gcactacgac gagaatcatt acagtggcgc aatatggctg ctctatgtca tgatcctggt
    38701 atggggcgct gactctggcg catatatgtt tggcaaattg tttggtaaac ataagctggc
    38761 accgaaggtt tctccgggta aaacctggca aggctttata ggtggactcg cgaccgcagc
    38821 ggtaatctca tggggttatg gcatgtgggc gaatctcgac gtcgctcccg tcaccttact
    38881 catttgctct attgtcgcag cgttagcctc agtgctcggc gatctgaccg agagtatgtt
    38941 taagcgtgaa gcaggaatta aggacagcgg tcatttaatt ccaggacacg gtggtatttt
    39001 agatcgtatt gatagcctga cggctgcggt accggtcttt gcttgcttgt tgttactggt
    39061 attcaggacg ctttaacgga aggtaatatg ctgagttttc tctgggattt ggcttcgttc
    39121 atcgttgcac tgggtgtact tatcaccgtg catgaatttg gtcatttctg ggttgcccgg
    39181 cgttgtggtg ttcgcgttga gcgtttctca atagggtttg gtaaggcgct ctggcggcga
    39241 actgataagc tcggcaccga atatgttatc gccctgatcc cgctgggcgg ttatgtcaaa
    39301 atgctggatg agcgcgcaga accggtcgtt ccggaactcc gccaccatgc cttcaataat
    39361 aaatctgtcg gccaacgagc ggcgattatt gccgcaggtc cggttgcaaa cttcattttt
    39421 gctatctttg cctactggct ggtttttatt attggtgtgc ctggcgtacg tccggtggtt
    39481 ggcgaaatag cagccaattc gatagctgcg gaagcacaaa ttgcaccagg tacggaacta
    39541 aaagccgtag atggtatcga aacgcctgat tgggatgccg tgcgtttgca gttggtcgat
    39601 aaaattggcg atgaaagcac caccattacg gtagcgccat ttggcagcga ccaacggcgg
    39661 gatgtaaagc tcgatttacg tcactgggcg tttgagcctg ataaagaaga tccggtaact
    39721 tcgctgggga ttcgtcctcg tgggccgcaa attgaacctg tactggaaaa tgtgcagcca
    39781 aactcggcgg caagcaaggc aggtttgcaa gcaggcgaca ggatcgttaa agtcgatggt
    39841 cagcccttaa cgcagtgggt gacctttgtg atgcttgtcc gggataaccc gggtaaatcc
    39901 ttagcgttag aaatcgaaag gcaggggagt cccttgtctt tgacattaat cccggagagt
    39961 aaaccgggta atggtaaagc gattggtttt gtcggtattg agccgaaagt cattcctttg
    40021 ccagatgagt ataaagttgt acgccagtat gggccgttca acgccatcgt cgaagccacg
    40081 gacaaaacgt ggcagctgat gaagctgacg gtcagtatgc tgggaaaatt gatcaccggt
    40141 gatgtgaaac tgaacaacct cagtgggccg atctctatcg ccaagggggc tgggatgaca
    40201 gcggaactcg gggttgttta ttacctgccg tttcttgcgc ttattagcgt gaacttaggg
    40261 ataattaacc tgtttccgtt gcccgtactt gacggggggc atctgctgtt ccttgcgatc
    40321 gaaaagatca agggcggacc ggtatccgag cgggttcaag acttttgtta tcgcattggc
    40381 tcgattttgc tggtgctgtt aatggggctt gcacttttca atgatttctc tcggttatga
    40441 gagttagtta ggaagaacgc ataataacga tggcgatgaa aaagttgctc atagcgtcgc
    40501 tgctgtttag cagcgccacc gtatacggtg ctgaagggtt cgtagtgaaa gatattcatt
    40561 tcgaaggcct tcagcgtgtc gccgttggtg cggccctcct cagtatgccg gtgcgcacag
    40621 gcgacacggt taatgatgaa gatatcagta ataccattcg cgctctgttt gctaccggca
    40681 actttgagga tgttcgcgtc cttcgtgatg gtgataccct tctggttcag gtaaaagaac
    40741 gtccgaccat tgccagcatt actttctccg gtaacaaatc ggtgaaagat gacatgctga
    40801 agcaaaacct cgaggcttct ggcgtgcgtg tgggcgaatc cctcgatcgc accaccattg
    40861 ccgatatcga gaaaggtctg gaagacttct actacagcgt cggtaaatat agcgccagcg
    40921 ttaaagctgt cgtgaccccg ctgccgcgca accgtgttga cctaaaactg gtgttccagg
    40981 aaggtgtgtc agctgaaatc cagcaaatta acattgttgg taaccatgct ttcaccaccg
    41041 acgaactgat ctctcatttc caactgcgtg acgaagtgcc gtggtggaac gtggtaggcg
    41101 atcgtaaata ccagaaacag aaactggcgg gcgaccttga aaccctgcgc agttactatc
    41161 tggatcgcgg ttatgcccgt ttcaacatcg actctaccca ggtcagtctg acgcctgata
    41221 aaaaaggtat ttacgtcacg gtgaacatca ccgaaggcga tcagtacaag ctttctggcg
    41281 ttgaagtgag cggcaacctt gccgggcact ccgctgaaat tgagcagctg actaagatcg
    41341 agccgggcga gctgtataac ggcaccaaag tgaccaaaat ggaagatgac attaaaaagc
    41401 ttcttggtcg ctatggttat gcctatccgc gcgtacagtc gatgcctgaa attaacgatg
    41461 ccgacaaaac cgttaaatta cgtgtgaacg ttgatgcggg taaccgtttc tacgtgcgta
    41521 agatccgttt tgaaggtaac gatacctcga aagatgccgt cctgcgtcgc gaaatgcgtc
    41581 agatggaagg tgcatggctg gggagcgatc tggtcgatca gggtaaggag cgtctgaatc
    41641 gtctgggctt ctttgaaact gtcgataccg atacccaacg cgttccgggt agcccggacc
    41701 aggttgatgt agtctacaag gtgaaagaac gtaacaccgg tagcttcaac ttcgggattg
    41761 gttacggtac tgaaagtggc gtgagcttcc aggctggtgt gcaacaggat aactggttag
    41821 gtacgggtta tgctgttggt atcaacggga ccaaaaacga ttaccagacc tatgctgaac
    41881 tgtcggtaac caacccgtac ttcaccgtag atggtgtaag cctcggtggt cgtctcttct
    41941 ataatgactt ccaggcagat gacgccgacc tgtccgacta taccaacaag agttatggta
    42001 cagacgtgac gttgggcttc ccgatcaacg aatataactc gctgcgtgca ggtctgggtt
    42061 atgtacataa ctccctgtcc aacatgcagc ctcaggttgc gatgtggcgt tatctgtact
    42121 ctatgggtga acatccgagc acctctgatc aggataacag cttcaaaacg gacgacttca
    42181 cgttcaacta tggttggacc tataacaagc ttgaccgtgg ttacttcccg acagatggtt
    42241 cacgtgtcaa cctgaccggt aaagtgacca ttccgggatc ggataacgaa tactacaaag
    42301 tgacgttaga cacggcgact tatgtgccga ttgatgacga tcacaaatgg gttgttctgg
    42361 gtcgtacccg ctggggttat ggtgatggtt taggcggcaa agagatgccg ttctatgaga
    42421 acttctatgc cggtggttcc agcaccgtgc gtggcttcca gtccaatacc attggtccga
    42481 aagcagttta cttcccgcat caggccagta attatgatcc ggactatgat tacgaatgtg
    42541 cgactcagga cggcgcgaaa gatctgtgta aatcggatga tgctgtaggc ggtaacgcca
    42601 tggcggttgc cagcctcgag ttcatcaccc cgacgccgtt tattagcgat aagtatgcta
    42661 actcggttcg tacttccttc ttctgggata tgggtaccgt ttgggataca aactgggatt
    42721 ccagccaata ttctggatat ccggactata gtgatccaag caatatccgt atgtctgcgg
    42781 gtatcgcatt acaatggatg tccccattgg ggccgttggt gttctcctac gcccagccgt
    42841 tcaaaaagta cgatggagac aaggcagaac agttccagtt taacatcggt aaaacctggt
    42901 aagtgttctc cacaaaggaa tgtagtggta gtgtagcgat gactttaggc gatcaatata
    42961 agatcgccgg gccacgcaaa gaactgcacc ctccggtgca aatgggatgg taaggagttt
    43021 attgtgaaaa agtggttatt agctgcaggt ctcggtttag cactggcaac ttctgctcag
    43081 gcggctgaca aaattgcaat cgtcaatatg ggcagcctgt tccagcaggt agcgcagaaa
    43141 accggagttt ctaacacgct ggaaaatgag ttcaaaggcc gtgccagcga actgcagcgt
    43201 atggaaaccg atctgcaggc taaaatgaaa aagctgcagt ccatgaaagc gggcagcgat
    43261 cgcaccaaac tggaaaaaga cgtgatggcc cagcgccaga cttttgctca gaaagcgcag
    43321 gcttttgagc aggatcgcgc acgtcgttcc aacgaagaac gcggcaaact ggttactcgt
    43381 atccagactg ctgtgaaatc cgttgccaac agccaggata tcgatctggt tgttgatgca
    43441 aacgccgttg cttacaacag cagcgatgta aaagacatca ctgccgacgt actgaaacag
    43501 gttaaataag taatgccttc aattcgactg gctgatttag cgcagcagtt ggatgcagaa
    43561 ctacacggtg atggcgatat cgtcatcacc ggcgttgcgt ccatgcaatc tgcacaaaca
    43621 ggtcacatca cgttcatggt taacccaaaa taccgtgagc atttaggctt gtgccaggcg
    43681 tccgcggttg tcatgaccca ggacgatctt cctttcgcga aaagtgccgc gctggtagtg
    43741 aagaatccct acctgactta cgcgcgcatg gcgcaaattt tagataccac gccgcagccc
    43801 gcgcagaaca ttgcacccag tgcggtgatt gacgcgacgg cgaagctggg taacaacgta
    43861 tcgattggcg ctaacgcggt gattgagtcc ggcgttgaac tgggcgataa cgtgattatc
    43921 ggtgccggtt gcttcgtagg taaaaacagc aaaatcggtg caggttcgcg tctctgggcg
    43981 aacgtaacca tttaccatga gatccagatc ggtcagaatt gcctgatcca gtccggaaca
    44041 gtggtaggcg cagacggctt tggttatgcc aacgatcgtg gtaactgggt gaagatccca
    44101 cagattggtc gcgtaattat tggcgatcgc gtggagatcg gtgcctgcac taccattgat
    44161 cgtggcgcgc tggatgacac tgttattggc aatggtgtga tcattgataa ccagtgccag
    44221 attgcacata acgtcgtgat tggcgacaat acggcagttg ccggtggcgt cattatggcg
    44281 ggcagcctga aaattggtcg ttactgcatg atcggcggag ccagcgtaat caacgggcat
    44341 atggaaatat gcgacaaagt gacggttacg ggcatgggta tggtgatgcg tcccatcact
    44401 gaaccaggcg tctattcctc aggcattccg ctgcaaccca acaaagtctg gcgcaaaacc
    44461 gctgcactgg tgatgaacat tgatgacatg agcaagcgtc tgaaatcgct tgagcgcaag
    44521 gttaatcaac aagactaacg ttccatcttt tgttcgccaa actttacggc ctgtctcatt
    44581 cttacgattg cggcaggccg tgttattatt gtcgtttctt atattttgac aggaagagta
    44641 tcttgactac taacactcat actctgcaga ttgaagagat tttagaactt ctgccgcacc
    44701 gtttcccgtt cttactggtg gatcgcgtgc tggattttga agaaggtcgt tttctgcgcg
    44761 cagtaaaaaa tgtctctgtc aatgagccat tcttccaggg ccatttccct ggaaaaccga
    44821 ttttcccggg tgtgctgatt ctggaagcaa tggcacaggc aacaggtatt ctggcgttta
    44881 aaagcgtagg aaaactggaa ccgggtgagc tgtactactt cgccggtatt gacgaagcgc
    44941 gctttaagcg cccggtcgtg cctggcgatc aaatgatcat ggaagtcact ttcgaaaaaa
    45001 cgcgccgcgg cctgacccgt tttaaagggg ttgctctggt cgacggtaaa gtagtttgcg
    45061 aagcaacgat gatgtgtgct cgtagccggg aggcctgata cgtgattgat aaatccgcct
    45121 ttgtgcatcc aaccgccatt gtggaagagg gcgcgtcgat tggcgcgaac gctcacattg
    45181 gtcctttttg tatcgttgga ccccatgtcg aaattggtga gggtaccgta ctgaaatctc
    45241 acgttgtcgt gaatggtcat actaaaattg gccgcgacaa tgagatttat cagttcgcct
    45301 ccatcggcga agtcaaccag gatcttaaat atgctggcga accgacccgt gtggaaatcg
    45361 gcgatcgtaa ccgcattcgc gaaagcgtca ccattcatcg tggcacagtc cagggcggtg
    45421 gattgacgaa ggtgggcagc gacaacttac tgatgatcaa cgcgcacatt gcgcacgatt
    45481 gtacggtagg taaccgctgt attctcgcca acaacgcaac gctggcgggt cacgtatcgg
    45541 ttgacgactt cgcgatcatc ggcggcatga ccgcagtcca tcagttctgc atcattggtg
    45601 cgcacgtgat ggttggcggc tgctccggtg tggcgcagga cgtccctcct tatgtcattg
    45661 cgcagggtaa ccacgcaacg ccgttcggtg tcaatatcga agggctgaag cgccgcggat
    45721 tcagccgtga ggcgattacc gctatccgca atgcgtataa gctgatttat cgtagcggta
    45781 aaacgctcga tgaagtgaaa ccggaaattg ctgaactggc ggaaacatat ccggaagtga
    45841 aagcctttac cgatttcttt gcacgctcaa cgcgcggtct gattcgttaa tgactgaaca
    45901 gcgtccatta acgattgccc tggtcgccgg agaaacctcc ggcgatatcc tgggggccgg
    45961 tttaatccgc gctctgaaag aacgtgtgcc caacgcccgc tttgttggtg ttgccgggcc
    46021 acgaatgcag gctgaaggct gcgaagcctg gtacgaaatg gaagaactgg cggtgatggg
    46081 cattgttgaa gtgctcggtc gtctgcgtcg cttactgcat attcgtgccg atctgacaac
    46141 gcgttttggc gaactgaagc cagatgtttt tgtcggtatt gatgcgcctg acttcaatat
    46201 tacccttgaa ggtaacctta aaaagcaggg tatcaaaacc attcattatg tcagtccgtc
    46261 cgtctgggcg tggcgacaga aacgcgtttt caaaataggc agagccaccg atctggtgct
    46321 cgcatttctg cctttcgaaa aagcgtttta tgacaaatat aacgtgccgt gccgttttat
    46381 cggtcacacc atggctgatg ccatgccgtt agatccagat aaaaacgccg cgcgtgatgt
    46441 gctggggatc cctcacgatg cccactgtct ggcattgttg ccgggaagcc gtggcgcgga
    46501 agtggagatg cttagcgccg atttcctgaa aacggcccag cttttgcgcc agacgtatcc
    46561 tgatctggag atcgtggtgc cgctggtaaa tgccaaacgc cgtgagcaat ttgaacgcat
    46621 caaagctgaa gtcgcgccag acctttcggt tcatatgctg gatggaatgg gccgtgaggc
    46681 gatggtcgcc agcgatgcgg cgctattggc gtcgggtacg gctgcactgg agtgcatgct
    46741 ggcgaaatgc ccgatggtgg tggggtatcg catgaagcct ttcaccttct ggttggcgaa
    46801 gcggctggtg aaaaccgatt atgtctcgct gccaaacctg ctggcgggca gagagttagt
    46861 taaagaatta ttgcaggaag agtgtgagcc gcaaaaactg gctgcggcgc tgttaccgct
    46921 gttggcgaac gggaaaacca gccacgcgat gcacgatacc ttccgtgaac tgcatcagca
    46981 gatccgctgc aatgccgatg agcaggcggc acaagccgtt ctggagttag cacaatgatc
    47041 gaatttgttt atccgcacac gcacctggtt gcgggtgtgg atgaagtcgg acgcgggccg
    47101 ttagttggcg cggtcgtcac cgctgcggtg atcctcgacc cggcgcgccc gattgccggg
    47161 ctgaatgatt ccaaaaagct gagcgaaaaa cgccgtctgg cgctctgcga agagatcaaa
    47221 gagaaagcgt tgagctggag tctgggccga gcggaacctc acgaaatcga cgaactgaat
    47281 attttacacg cgaccatgct ggcgatgcag cgtgccgtcg ctgggctgca tattgcgccg
    47341 gaatatgtgt tgattgatgg taaccgctgc ccgaaattac cgatgcctgc gatggctgtg
    47401 gtgaaaggcg atagccgcgt accggaaatc agtgccgcgt ctatcctggc gaaagtgacg
    47461 cgtgacgccg aaatggcggc gctggatatt gttttcccgc aatatggttt tgcccaacat
    47521 aaagggtatc caaccgcttt tcatctggaa aaactggctg aatatggcgc gactgaacat
    47581 catcgtcgca gctttgggcc tgtcaaacgc gtactgggac ttgcgtcctg attcttgtgt
    47641 cgagattaag taaaccggaa tctgaagatg tctgaaccac gtttcgtaca cctgcgggtg
    47701 cacagcgact actcgatgat cgatggtctg gccaaaaccg caccgctggt aaaaaaggcg
    47761 gcggcgttgg gtatgccagc actggcgatc accgatttca ccaacctttg cggtctggtg
    47821 aagttctacg gagcgggaca tggcgcaggg attaagccta tcgtcggggc agattttaat
    47881 gtccagtgcg acctgctggg tgatgagtta acccacctga cggtgctggc ggcgaacaat
    47941 accggctatc agaatctgac gttgctgatc tcaaaagcgt atcagcgcgg gtacggtgca
    48001 gctgggccga tcatcgatcg cgactggctt atcgaattaa acgaagggtt gatccttctt
    48061 tccggcgggc gcatgggcga cgtcggacgc agtcttttgc gtggtaacag cgcgctggta
    48121 gatgagtgtg tcgcgtttta tgaagaacac ttcccggatc gctattttct cgagctgatc
    48181 cgcaccggca ggccggacga agaaagctat ctgcacgctg cggtggaact ggcggaagca
    48241 cgcggtttgc ccgttgtggc gaccaacgac gtgcgcttta tcgacagcag cgactttgac
    48301 gcacacgaaa tccgcgtcgc gatccacgac ggctttaccc tcgacgatcc taaacgcccg
    48361 cgtaactatt cgccgcagca atatatgcgt agcgaagagg agatgtgcga gctttttgcc
    48421 gacattcccg aagcccttgc caacaccgtt gagatcgcca aacgttgtaa cgtaaccgtg
    48481 cgtcttggtg aatacttcct gccgcagttc ccgaccgggg acatgagcac cgaagattat
    48541 ctggtcaagc gtgcaaaaga ggggctggaa gagcgtctgg cctttttatt ccctgacgag
    48601 gaagaacgtg ttaagcgccg cccggaatat gacgaacgtc tggagactga acttcaggtt
    48661 atcaaccaga tgggcttccc gggttacttc ctcatcgtta tggaatttat ccagtggtcg
    48721 aaagataacg gcgtaccagt agggccaggc cgtggctccg gtgcgggttc actggtggcc
    48781 tacgcgctga aaatcaccga cctcgatccg ctggaatttg acctgctgtt cgaacgtttc
    48841 cttaacccgg aacgtgtctc catgcctgac ttcgacgttg acttctgtat ggagaaacgc
    48901 gaccaggtta tcgagcacgt agcggacatg tacggtcgtg atgcggtatc gcagatcatc
    48961 accttcggta cgatggcggc gaaagcggtg atccgcgacg taggtcgcgt gctggggcat
    49021 ccgtacggct ttgtcgatcg tatctcgaaa ctgatcccgc ccgatccggg gatgacgctg
    49081 gcgaaagcgt ttgaagccga gccgcagctg ccggaaatct acgaagcgga tgaagaagtt
    49141 aaggcgctga tcgacatggc gcgcaaactg gaaggggtca cccgtaacgc cggtaagcac
    49201 gccggtgggg tggttatcgc gccgaccaaa attaccgatt ttgcgccgct ttactgcgat
    49261 gaagagggca aacatccggt cacccagttt gataaaagcg acgttgaata cgccggactg
    49321 gtgaagttcg acttccttgg tttgcgtacg ctcaccatca tcaactgggc gctggagatg
    49381 atcaacaagc ggcgggcgaa gaatggcgag ccgccgctgg atatcgccgc gatcccgctg
    49441 gatgataaga aaagcttcga catgctgcaa cgctcggaaa ccactgcggt attccagctt
    49501 gaatcgcgcg gcatgaagga cctgatcaag cgtctgcaac ctgactgctt cgaagatatg
    49561 atcgcactgg tggcactgtt ccgccccggt ccgttgcaat cagggatggt ggataacttt
    49621 atcgaccgta aacatggtcg cgaagagatc tcctatccgg acgtgcagtg gcagcatgaa
    49681 agcctgaaac cggtactgga gccaacctac ggcatcatcc tgtatcagga acaggtcatg
    49741 cagattgccc aggtgctttc tggttatacc ctcggtggcg cagatatgct gcgtcgtgcg
    49801 atgggtaaga aaaagccgga agagatggct aagcagcgtt ctgtatttgc tgaaggtgca
    49861 gaaaagaacg gaatcaacgc cgaactggcg atgaaaatct tcgacctggt ggagaaattc
    49921 gcgggttacg gatttaacaa atcgcactct gcggcctatg ctttggtgtc atatcaaacg
    49981 ttatggctga aagcgcacta tccggcagag tttatggcgg cggtaatgac cgccgatatg
    50041 gacaataccg agaaggtggt gggcctggtg gatgagtgct ggcggatggg gctgaaaatc
    50101 ctgccaccag atataaactc cggtctttac catttccacg tcaacgacga cggcgaaatc
    50161 gtgtatggta ttggcgcgat caaaggggta ggtgaaggtc cgattgaggc catcatcgaa
    50221 gcccgtaata aaggcggcta cttccgcgaa ctgtttgatc tctgcgcccg aaccgacacc
    50281 aaaaagttaa accggcgggt gctggaaaaa ctgatcatgt ccggggcgtt tgaccgtctt
    50341 gggccacacc gtgcggcgct gatgaactcg ctgggcgatg cgttaaaagc ggcagatcaa
    50401 cacgcgaaag cggaagctat cggtcaggcc gatatgttcg gcgtgctggc cgaagaaccg
    50461 gaacaaattg aacaatccta cgccagctgc caaccgtggc cggagcaggt ggtattagat
    50521 ggggaacgtg aaacgttagg tctgtacctg acgggacacc ctatcaacca gtatctaaaa
    50581 gagattgagc gttatgtcgg aggcgtaagg ctgaaagaca tgcacccgac agaacgtggt
    50641 aaagtcatca cggctgcggg gctcgttgtt gccgcgcggg ttatggtcac caagcgcggc
    50701 aatcgtatcg gtatctgcac gctggatgac cgttccgggc ggctggaagt gatgttgttt
    50761 actgacgccc tggataaata ccagcaattg ctggaaaaag accgcatact tatcgtcagc
    50821 ggacaggtca gctttgatga cttcagcggt gggcttaaaa tgaccgctcg cgaagtgatg
    50881 gatattgacg aagcccggga aaaatatgct cgcgggcttg ctatctcgct gacggacagg
    50941 caaattgatg accagctttt aaaccgactc cgtcagtctc tggaacccca ccgctctggg
    51001 acaattccag tacatctcta ctatcagagg gcggatgcac gcgcgcggtt gcgttttggc
    51061 gcgacgtggc gtgtctctcc gagcgatcgt ttattaaacg atctccgtgg cctcattggt
    51121 tcggagcagg tggaactgga gtttgactaa tacaggaata ctatgagtct gaatttcctt
    51181 gattttgaac agccgattgc agagctggaa gcgaaaatcg attctctgac tgcggttagc
    51241 cgtcaggatg agaaactgga tattaacatc gatgaagaag tgcatcgtct gcgtgaaaaa
    51301 agcgtagaac tgacgcgtaa aatcttcgcc gatctcggtg catggcagat tgcgcaactg
    51361 gcacgccatc cacagcgtcc ttataccctg gattacgttc gcctggcatt tgatgaattt
    51421 gacgaactgg ctggcgaccg cgcgtatgca gacgataaag ctatcgtcgg tggtatcgcc
    51481 cgtctcgatg gtcgtccggt gatgatcatt ggtcatcaaa aaggtcgtga aaccaaagaa
    51541 aaaattcgcc gtaactttgg tatgccagcg ccagaaggtt accgtaaagc actgcgtctg
    51601 atgcaaatgg ctgaacgctt taagatgcct atcatcacct ttatcgacac cccgggggct
    51661 tatcctggcg tgggcgcaga agagcgtggt cagtctgaag ccattgcacg caacctgcgt
    51721 gaaatgtctc gcctcggcgt accggtagtt tgtacggtta tcggtgaagg tggttctggc
    51781 ggtgcgctgg cgattggcgt gggcgataaa gtgaatatgc tgcaatacag cacctattcc
    51841 gttatctcgc cggaaggttg tgcgtccatt ctgtggaaga gcgccgacaa agcgccgctg
    51901 gcggctgaag cgatgggtat catcgctccg cgtctgaaag agctgaaact gatcgactcc
    51961 atcatcccgg aaccgctggg cggcgctcat cgtaacccgg aagcgatggc ggcatcgttg
    52021 aaagcgcaac tgctggcgga tctggccgat ctcgacgtgt taagcactga agatttaaaa
    52081 aatcgtcgtt atcagcgcct gatgagctac ggttacgcgt aattcgcaaa agttctgaaa
    52141 aagggtcact tcggtggccc ttttttatcg ccacggtttg agcaggctat gattaaggaa
    52201 ggattttcca ggaggaacac atgaacatca ttgccattat gggaccgcat ggcgtctttt
    52261 ataaagatga gcccatcaaa gaactggagt cggcgctggt ggcgcaaggc tttcagatta
    52321 tctggccaca aaacagcgtt gatttgctga aatttatcga acataaccct cgaatttgcg
    52381 gcgtgatttt tgactgggat gagtacagtc tcgatttatg tagcgatatc aatcagctta
    52441 atgaatatct cccgctttat gccttcatca acacccactc gacgatggat gtcagcgtgc
    52501 aggatatgcg gatggcgctc tggttttttg aatatgcgct ggggcaggcg gaagatatcg
    52561 ccattcgtat gcgtcagtac accgacgaat atcttgataa cattacaccg ccgttcacga
    52621 aagccttgtt tacctacgtc aaagagcgga agtacacctt ttgtacgccg ggtcatatgg
    52681 gcggcaccgc atatcaaaaa agcccggttg gctgtctgtt ttatgatttt ttcggcggga
    52741 atactcttaa ggctgatgtc tctatttcgg ttaccgagct tggttcgttg ctcgaccaca
    52801 ccgggccaca cctggaagcg gaagagtaca tcgcgcggac ttttggcgcg gaacagagtt
    52861 atatcgttac caacggaaca tcgacatcga acaaaattgt gggtatgtac gccgcgccat
    52921 ccggcagtac gctgttaatt gaccgcaatt gtcataaatc gctggcgcat ctgttgatga
    52981 tgaacgatgt agtgccagtc tggctgaaac cgacgcgtaa tgcgttgggg attcttggtg
    53041 ggatcccgcg ccgtgaattt actcgcgaca gcatcgaaga gaaagtcgct gctaccacgc
    53101 aagcacaatg gccggttcat gcggtgatta ccaactccac ctatgatggc ttgctctaca
    53161 acaccgactg gatcaaacag acgctggatg tcccgtcgat tcacttcgat tctgcctggg
    53221 tgccgtacac ccattttcat ccgatctacc agggtaaaag tggtatgagc ggcgagcgtg
    53281 ttgcgggaaa agtgatcttc gaaacgcaat cgacccacaa aatgctggcg gcgttatcgc
    53341 aggcttcgct gatccacatt aaaggcgagt atgacgaaga ggcgtttaac gaagccttta
    53401 tgatgcatac caccacctcg cccagttatc ccattgttgc ttcggttgag acggcagcgg
    53461 cgatgctgcg tggtaatccg ggcaaacggc tgattaaccg ttcagtagaa cgagcgctgc
    53521 attttcgcaa agaggtccag cggctgcggg aagagtctga cggctggttt ttcgatatct
    53581 ggcaaccgcc gcaggtggat gaagccgaat gctggcccgt tgcgccaggc gaacgatggc
    53641 acggctttaa cgatgcggat gccgatcata tgtttctcga tccggttaaa gtcactattc
    53701 tgacaccggg gatggacgag cagggcaata tgagcgagga ggggatcccg gcggcgctgg
    53761 tggcaaaatt cctcgacgaa cgtgggatcg tagtagagaa aaccggcccg tataacctgc
    53821 tgtttctttt tagtattggc atcgataaaa ccaaggcaat gggattattg cgtgggttga
    53881 cggaattcaa acgctcttac gatctcaacc tgcggatcaa aaatatgcta cccgatctct
    53941 atgctgaaga tcccgatttc taccgcaata tgcgtattca ggatctggcg caagggatcc
    54001 ataagctgat tcgtaaacac gatctttccg gtttgatgtt gcgggcattt gataccttgc
    54061 cggagatgat catgacgcca catcaggcat ggcagagaca gattaaaggc gaagtggaaa
    54121 ccattgcgct ggaacaactg gtcggtagag tatcggcaaa tatgatcctg ccttatccac
    54181 ctggcgtacc gctgctgatg ccaggagaaa tgctgaccaa agagagccgc acggtactcg
    54241 attttctact gatgctttgt tccgtagggc aacattaccc cggttttgaa acggatattc
    54301 acggcgcgaa acaggacgaa gacggcgttt accgcgtacg agtcctaaaa atggcgggat
    54361 aacttgccag agcggcttcc gggcgagtaa cgtgctgtta acaaataaag gagacgttat
    54421 gctgggttta aaacaggttc accatattgc gattattgcg acggattatg cggtgagcaa
    54481 agcgttttac tgcgatattc ttggtttcac gctgcaaagc gaagtctatc gcgaagcgcg
    54541 cgactcgtgg aaaggggatt tggcgcttaa tgggcaatat gtgattgagc ttttctcatt
    54601 tccgttcccg ccggagcgcc ccagccgacc ggaagcttgc ggcctgcgtc atctggcttt
    54661 tagcgttgat gatatcgatg cggcagtggc gcaccttgaa agccataacg tgaagtgtga
    54721 agccatccgt gtcgatccat acacgcaaaa acgcttcacg ttctttaacg atccggacgg
    54781 gctgccgttg gaactgtatg agcagtaagg cttgtcatcg ccgcatttgc ccggtaacgt
    54841 gccgggcatt gctactgtaa aatcgcatca tcatgacact cacgctcaat agacaacttc
    54901 tctcctcacg ccagattctg gtggccttta gcggcgggct tgactccacc gttctgctgc
    54961 atcagttggt gcagtggcgg atggaaaatc cgggcgtcac tctgcgcgct atccatgtgc
    55021 atcacggttt aagtgctaat gccgatgcct gggttaggca ttgcgagaac atctgccaac
    55081 agtggcaggt gccgctggtg gtcgagcgcg tgcaacttgc gcaagaagga ctgggcattg
    55141 aggcccaggc gcggcaggca cgttatcagg catttgcccg caccttgtta cccggtgaag
    55201 tgctggtcac cgcgcaacat ctcgacgatc aatgtgaaac ctttctgctg gcgctaaaac
    55261 gcggtagcgg ccctgccggg ctttcggcta tgggggaagt ttcggagttt gccggaacac
    55321 ggcttatccg cccgttgctc gcccgcacgc ggggagaact ggtgcagtgg gcgcgtcagt
    55381 atgatttacg ctggattgaa gacgaaagta atcaggatga tagctacgat cgtaacttcc
    55441 tgcgcctgcg cgtggtgccg ttattgcagc agcgttggcc gcattttgcc gaagcaacgg
    55501 cccgcagcgc tgcgctttgc gctgaacaag agagcctgtt ggatgaactg ctggcggatg
    55561 atatagcaca ctgtcaaacg tcgcagggga cgctgcagat tgcgccaatg ctggcgatga
    55621 gtgatgcccg ccgcgcggcg attatccgcc gctggctggc agggaagaat gcaccgatgc
    55681 cttcccgcga cgcgttggtg aggatctggc aggaagtggc gctggcgcgg gaagatgctt
    55741 ccccctgttt acgcctgggc gcgtttgaaa tccgccgcta tcagtcgcaa ctgtggtgga
    55801 ttaaatccgt cactgggcaa agcgaaacca ttgtgccgtg gcagacgtgg ctacaaccgc
    55861 tggaattacc ggcgggactg ggaagtgtac agcttactgc gggaggcgat attcgccctc
    55921 cgcgtgcaga cgaagcggtc agcgtgcgtt tcaaagcgcc aggattgctg catattatcg
    55981 ggcgcaacgg cggacgtaag ctgaagaaaa tctggcaaga gctgggtgtg ccgccgtggc
    56041 tacgtgacac cacgccactg ctgttttatg gcgaaacgtt gattgcggcg gcaggggtat
    56101 ttgtgacgca agaaggtgtg gctgaaggtg agaatggcgt cagttttgtc tggcagaaaa
    56161 cgcttagtta agtgaaagcc ggataagacg cttcaaacgt cgcatccggc gaaagtcaat
    56221 caggactcgc tcaccaccac cgtaccgatt tccgggtggc taaagctggt aattttatcc
    56281 agacgcagct cgcgggttgt accagcggcc tcgacgacca gatactccac atttttgcgg
    56341 gagactaaat cgctggcctt cgcctgcaat ttttcgccat ctttcagctc aagtgtcagc
    56401 attaaatgat gctggcaggc gagctcaaga ttatcgtaat catcacaatt gattggttga
    56461 tacgtatcat tcattgacat aatcgctcac cagtaagttt gccgcagcgt atgctgcttt
    56521 ttccctgaca gcctcagaaa gggcgtcgtc ggcagccatt tcattcagca ctttcaaaac
    56581 gcagcccagc gcgtccggaa cgtatcctaa gtctccgctg gcgatttccg cgtaccgctt
    56641 gcgtattaac tcacaatatt tttccacatg ccctcctgtc agcactctga cttaaccgtg
    56701 gatgcaagtc taagcctacg aagataaact ctgtttcgca aggtgactat accacactca
    56761 tttctgcaat atcagcgccg caactgcacg tattccgtta caatggcctc ctgattcgaa
    56821 aggagttttc ttatggcgct taaagcgaca atttataaag cgacggttaa tgtggccgat
    56881 ctcgaccgca accagtttct cgatgcctct ctgacgctgg cgcgccaccc ttcagaaacc
    56941 caggagcgca tgatgctgcg cctgctggcg tggctaaaat atgccgatga acgtctgcaa
    57001 tttacccgtg gtttgtgtgc cgatgatgag ccggaagcgt ggctgcgtaa cgatcatttg
    57061 ggtattgatt tgtggattga gctggggctg ccggatgagc gacggattaa gaaagcctgc
    57121 acccaggccg cagaagtggc gctgtttgcc tataatagtc gggcggcgca aatctggtgg
    57181 cagcaaaatc agagcaaatg cgcgcagttt tccaatcttt ccatctggta tctggacgat
    57241 gaacaactgg cgaaagtgag cgcctttgcc gatcgtacca tgacgctgca ggcaacgatt
    57301 caggatggcg tgatctggtt atcggatgat aagaataatc tggaagtgaa cttaaccgcc
    57361 tggcaacaac cttcatgatt gtgatttccc gacatgttgc tatccccgat ggtgagcttg
    57421 agatcaccgc cattcgtgcg cagggcgcgg gcgggcagca tgttaataag acctcaacgg
    57481 ctattcatct gcgttttgac attcgggcgt ccagcctgcc agagtattac aaagaacgtc
    57541 tgctcgccgc cagccatcac ttgatcagca gtgatggcgt gattgtcatt aaggcacagg
    57601 aataccgcag tcaggaactg aaccgcgaag cggctctggc ccggctggtg gcagtgatta
    57661 aagatttaac aacagaacaa aaagcccgac gacccacgcg ccccacccgt gcatcgaaag
    57721 agcgcaggct ggcatcgaaa gcacaaaaat caagcgtgaa ggcgatgcgc ggcaaagtgc
    57781 gcagcggtcg ggaataaaaa gaaggaatgg atggtgaaaa aagcgatagt gacagcgatg
    57841 gctgtaatca gcctctttac tctgatgggt tgtaataatc gggccgaagt cgatacgctt
    57901 tctccggcgc aggctgccga actgaaaccg atgccgcaaa gttggcgcgg cgtgctgccg
    57961 tgtgctgatt gcgaaggaat cgaaacctct ctgttcctcg aaaaagacgg aacatgggtg
    58021 atgaatgagc gttatctcgg tgctcgtgaa gaaccttcct ccttcgcttc ctacggtaca
    58081 tgggcgcgaa ccgctgacaa gctagtatta accgacagca aaggtgaaaa gtcatattat
    58141 cgggcgaaag gcgatgcgct ggagatgctc gatcgtgaag gcaatccgat tgaatcgcag
    58201 ttcaactata cgctagaacc ggcacaatcc agtttaccta tgacgccgat gaccctgcgg
    58261 ggcatgtatt tttatatggc tgatgcggcg accttcactg attgcgcgac cggaaaacgt
    58321 ttcatggtag cgaataacgc agagctggag cgtagctacc tggctgcgcg cggtcacagt
    58381 gaaaaaccgg tgttattgtc agtagaaggt cactttacgc ttgagggtaa tccggatacc
    58441 ggtgcgccga ctaaagtatt ggcacccgat acggcgggta aattttatcc gaaccaggat
    58501 tgcagtagtt tggggcagta attgaaagcc ggagcattct ccggctttta tcactgagtc
    58561 agcccaacaa agcgacccgc cttgatataa atccctggct tcatgtgccc aacatactgt
    58621 aactgcgttt ctggcttaaa cgcagagata tcgccagtac gcatatgcat cagatcggca
    58681 gggctaatcc agcctgattg cgccagcgta agcggatgcc cggctttggc aaatgcatca
    58741 gtgacaaatt ccgaacaaaa ccacgacttt ttgtctcctt cgcccacact gcttaactgc
    58801 gctttcgcca ggccgctgac gcactgttga cgaaaatcct cggagaacgg attcagtgag
    58861 cacacctggc gagtcaccat aaagggaata aattcgacaa tgccgcgata gttataaccg
    58921 ctatctttga ttttattagc aaacgcggtg atatctgtgg cttgttgcgg ggtaagatcc
    58981 gggactcgta agacgaaaag cttatcactg tgcttcatcg cttttttaag ggaaacgatc
    59041 tggacgccag cgcctgtcgc ttctgcaacg ttattatcac ccagaaagat tgcaacgtga
    59101 ctcacagagg aagtgctgaa gacgcggatt ccaaatgagg ttaccccaag gcttgaggag
    59161 aacagcaaat cgccgggttt gagatcaggt gccgttattt cttttattga ttgttcggtg
    59221 aaagagcttt gatgctggaa tttgactacc catgtttttg cctcgacatc taccgctgtg
    59281 gcagaggagt ctggctggct gatatcaacg gtacaggccg aaaggaaaag aaagcagggg
    59341 agaagcaggc ggcaatgcgc ctttggttta tccattttat acaatccatg taaaaaaggg
    59401 ccctgaaatt caggaccctt tctggcatca gcctttaatc tgtttcacca gatattcgac
    59461 gatgtcacca gtcttaatta actgtttctc gccgttacga cgatatttat attcgatatc
    59521 atcgttgtcg aggttacggt cgcccagcac aatagtgtgc ggaataccga tcagttccat
    59581 atcagcaaac atcacgcccg gacgctcttt gcggtcatcc agcagcactt cgataccttg
    59641 tgcacgcagt tcgctgtaca gtttctcagc aagctcttgt acgcggaagg atttgtgcat
    59701 gttcatcggc agaatcgcca cctggaaagg cgcgatagcg tcaggccata cgatgccgcg
    59761 ttcgtcatag ttctgctcaa ttgccgcagc cacaacgcgc gttaccccga taccgtagca
    59821 acccatcgtc aggatttgat tacggccatc ttcaccctgt acggaggctt tcagtgcttc
    59881 ggagtacttg gtacccagct ggaagatgtg accaacttcg ataccacgtt tgatcagcag
    59941 cgtaccctga ccatccgggc ttggatcgcc agccaccacg ttgcggatat cagcaatttc
    60001 cggggtagcg acatcgcgat cccagttgat accgaagtag tgtttaccat cgatgttagc
    60061 accagcagcg aaatcactca tcgccgcaac ggtacggtca atcaccaccg gaattggcat
    60121 gtttaccgga cccagtgagc cagggccggc tttaaccacg gcacgaattt cttcttcggt
    60181 cgcgaaagtc agcgggcttg caacctgcgg cagtttttct gctttaactt cgttcagctc
    60241 gtggtcaccg cgcaccagca gcgcaaccag cgggaagcta ctgccttcaa ccgctttaac
    60301 cagcagggtc ttaaccgttt tctcaatcgg cagattgaac tgttcaacca gttccgcgat
    60361 ggtcttcgcg ttcggcgtat caaccagcgt catttcctgg gtagcagcag cgcgcggttc
    60421 tttcggcgcg atagcttctg ccagttcaat gttcgctgca tagtcagagg tgtcggagaa
    60481 gaccacatcg tcttcaccgc tttgcgccag cacctggaat tcgtgagagg cgctaccgcc
    60541 gatagaaccg gtatcggcct gtacggcgcg gaaatccagc cccatgcggc tgaagatttt
    60601 gctgtaggcc gcatacattg catcgtaggt ttcctgcagg gattcctgag aggtatggaa
    60661 agagtaagca tctttcatca ggaattcgcg ggaacgcatg acgccgaaac gcgggcgcac
    60721 ttcatcacgg aacttagtct ggatctgata gaagttcagc ggcagctgtt tgtaagagct
    60781 cagctcgtta cgaatcaggt cggtaatcac ttcttcatga gttgggccaa gtacgaacgg
    60841 acggtcacca cggtcagcaa tacgcagcaa ttccgggcca tactgttccc aacgaccact
    60901 ctcttgccac agttcagatg gctgaaccac cggcattaac acctcaatcg caccggcgtt
    60961 gttcatctct tcacgcacga tgttttcgac ttttttcaga acgcgcacgc cggtcggcag
    61021 ccaggtgtat aacccggagg ccagcttgcg gatcatcccg gcgcgcagca tcagctgatg
    61081 gctgatcacc tcggcgtcgg caggtgtctc cttgagagtg gagagcaggt attggctagt
    61141 acgcatgttg ttacggttcc agttggaagg tagaacaggc tcaaggtgag cctgggacaa
    61201 aaaaagtgat ttagtttacc agtgcaaaag aaatgtcaaa agagaagggc gtgaatttaa
    61261 cgcggttcca gcgcaaagac ttcaaaacct gcgtcggtga cgcgccagcg aacgttaaaa
    61321 tcatgcagcc agacggcata ggttttgccc gtttcctcac ctttacgata ggccgggcgc
    61381 gggtcctgcg ctagtacttc gcggataaac agcgttaact gcggataacg cttctccagc
    61441 gtcaaaagct gcttttcgac ctctgcggta aaactcaccg ccatctctgc agctggcgca
    61501 ctttgcgcat agctggcact ggcatttgga agcgattcgg caaaggggag atacggtttg
    61561 atatccacta ccggcgtacc atcgaccaga tccagactgc cgagcttcag aatcacgctg
    61621 tctttatggc aaacaacctc tttcagctct accagcgaca taccaattgg gttagggcga
    61681 aaagtggagc gtgttgcgaa aacccccatt ctggcattac cgccaagacg aggcggacgc
    61741 acagtcggac gccagccgcc ttccatcgtt tgatgaaaga cgaaaagtat ccataaatgg
    61801 ctgaacgctt ccaggccgcg aacggcgtcg gcttggttgt agggagcaat gagatgcagt
    61861 tcgccgttgg cgctttttac cagacctggt tggcgcggaa cggcgaattt ttctttatag
    61921 ggcgagcgaa taacgcctat ttgctcgaac tggaaactgc tcatttcgcc gtaatgttaa
    61981 gcgcagaacc gatacataca gcctgacgat agcagcctgg cgtaccgctg gtgacttcgc
    62041 agctatgcag taataccgca ttggctttca ttttcgaggc gttgatttgc atccgcttac
    62101 gcgcggttgg aatgctcggc ggagagtcct gattagaggc ttggcaagag tcgccactga
    62161 cttcaccgag atcgcggaac ggtttgccga ctaattcttc tgcattggta taaattcgga
    62221 ccggcgtggc gcgcggcact ttcggttttg cagactccgc tttcggctgg ggtgcagtgc
    62281 tttgaacggg ttcgacaggg gatctgctta acatggaaca gccgcttagc atgagtgcta
    62341 ctaaacagat cggtaaagca cgcatagtat ttcctcaatg tatgatcaaa acgtcaatat
    62401 tgaatcagga gcttgtaaaa atgacaagac gggcaagcgc ccgtcctgaa tgatattaca
    62461 aattgtggaa acagcctaaa aattaccagc ctttaacagc gccgccgtta aacactttgt
    62521 ttgctgcttc gtaaacttcg tcagactgat aagcctggac gaatttcttc acgttctcgg
    62581 cgtctttgtt atcttcacgc gttacgatca ggtttacgta cggggactct ttatcttcga
    62641 caaagatacc gtctttcgcc ggagtcaggc caatctggct ggcataggtg gtattgataa
    62701 ctgccagagc gatttgcgcg tcgtccagag aacgcggcag ctgcggtgct tccagctcaa
    62761 caattttcag atttttgggg ttctcaacaa catcaagaac ggtcggcagc aggccaacgc
    62821 catctttcag tttgatcaag cccacttttt gcagcagcag cagtgaacga ccaaggttag
    62881 ttgggtcgtt tggcacggca acctgcgaac catcctgcag ttcatccagt gatttgattt
    62941 tcttggagta acctgcaatc ggataaacaa aagtgttgcc tactgcgacc agtttgtagc
    63001 cacgatcttt cagttgctga tcaaggtacg gtttatgctg gaaggcgttg gcgtcgatat
    63061 cgcctttgct caatgcttcg tttggcagaa catagtcgtt gaatgttacc agctcaacgt
    63121 ccaggccata tttgtctttc gcaactttct gcgcgacttc tgcaacctgc tgttcggcac
    63181 caacaatcac gccgacttta atgtggtttg gatctttttc atcctgaccg cagcctacca
    63241 gtgccagtga tccaatcagg gctcccactg ccgcaaaggt tttgaatttg aacgccatac
    63301 cttattcctt cttcaattat ttatgttgtg ttgaacgtta cttgcgagtg acagcccgga
    63361 cgatgcggtc gcctgcgaac tgaattaaat aaaccagaat gaccagcaat accagtaccg
    63421 tattcatcac cgtcgcgttg tagccgatgt agccatactg atagccaatc tgacctaaac
    63481 caccggcacc gactgcacca cccatcgcgg aataaccgac cagggtaatc agggtgatag
    63541 ttgccgcatt taccagaccc ggcaacgctt ccggtaacag cactttacgg acgatctgca
    63601 ttggcgtggc acccattgcg cgggaagctt caattaaccc ggttgggatc tccagcagag
    63661 cgttctcgac catacgggca ataaacggtg ctgcaccaac ggttaacgga acaatcgctg
    63721 cctgcaaacc aatcgatgta ccaacaataa cgcgggtaaa cggaatcatc cataccagca
    63781 agataatgaa cgggatggaa cggaaaatgt tcacaatcgc agaaacggta cgatacagct
    63841 tcgcgttagc aataatttgc cccggacgcg tgacataaag caggacgcca accggcagac
    63901 caatcacaaa tccaaaaaaa ccggatacga aggtcattgc cagcgtttcc catacgccac
    63961 gaaccagcag ccacatcatc ggctcagaca taacccagta cctctacttt tacatggtgt
    64021 tcctgcagcc aggtaatggc ggcttgcgta tcttgttgtg tgccgtgcat ttcagtcagc
    64081 atgatgccga acttaacgcc accggcgtaa tccatctgcg cgctaataat gttgttgttg
    64141 acgttgaaac gacgcgcggt ttcagaaagc agtggggcat cgaccgattg atcggtaaac
    64201 tccagacgca acatcggcac gcagtcagtg aatggctccg cttgcagacg ttcctggtaa
    64261 tcttccggga tatccagatg cagggtcgac tgaataaact tctgcgccag cggcgttttc
    64321 ggatgcgaga acacttcact taccgtgtcc tgctcgatca gttcgccatt gctgatgacc
    64381 gccacgcaat cacaaatacg cttcacaacg tccatttcgt gggtgatcaa cagaatcgtc
    64441 aaacccagac ggcggttgat gtctttcagc agttcgagaa tagaacgtgt cgttgccgga
    64501 tccagcgcgc tggtggcctc atcacacagc aatactttgg gattgctggc taacgcacgg
    64561 gcaatcgcca cacgctgttt ctgcccaccg gaaagattcg acgggtagct atcatgctta
    64621 tcgccaagac caaccaatga cagcaattcc gtcacgcgac gtttgatctc atctttcggt
    64681 gtgttgtcca gctccagcgg cagagccacg ttgccaaaaa cagtacgcga agagagcagg
    64741 ttaaaatgct ggaaaatcat accaatctgg cggcgagctt tggtcaactc ggattctgac
    64801 agcgtggtca gttcctggcc atcgaccagc acgctaccct cggttgggcg ctccagcagg
    64861 tttacacaac gtataagcgt actcttaccc gcgccagagg caccgataac gccataaatt
    64921 tgtccagctg gcacatgcag gctgacgttg ttcaacgcct ggatggtgcg ggtgccctgg
    64981 tggaacactt tggtgatatt cgaaagtttt atcattgatt atttattatc gtcattaagt
    65041 tagtcgtggc atttcgaatg cctggaacgg gcaacgcctt caatgaaatg gatgttaagg
    65101 catccagacg tctaaatcaa tcaggtttat gcgaagagca ctttcttgct ggtcgaaaca
    65161 tgcgatacta gcgtcacatg ccttattaag gagctataaa aggtggcgaa gagcgtaccc
    65221 gcaatttttc ttgaccgtga tggcaccatt aatgtcgatc acggctatgt ccatgagatc
    65281 gacaactttg aatttatcga cggtgttatt gacgccatgc gcgagctaaa aaaaatgggc
    65341 tttgcgctgg tggtagtaac caaccagtct ggcattgctc gcggtaaatt tactgaagca
    65401 cagtttgaaa cgctgaccga gtggatggac tggtcgctgg cggaccgcga tgtcgatctg
    65461 gatggtatct attattgccc gcatcatccg cagggtagtg ttgaagagtt tcgccaggtc
    65521 tgcgactgcc gcaaaccaca tccggggatg cttttgtcag cacgcgatta tttgcatatt
    65581 gatatggccg cttcttatat ggtgggcgat aaattagaag atatgcaggc agcagctgcg
    65641 gcgaacgtgg gaacaaaagt gctggtgcgt acgggtaaac ctgttacgcc tgaagcagaa
    65701 aacgcggcgg attgggtgtt aaatagcctg gcagacctgc cgcaagcgat aaaaaagcag
    65761 caaaaaccgg cgtaatgatt aaaagatgag cggttgaaat aaaaatgcat ttttccgctt
    65821 gtcttcctga gccgactccc tataatgcgc ctccatcgac acggcggatg tgaatcactt
    65881 cacacaaaca gccggttcgg ttaaagagaa aaatcctgaa attcagggtt gactctgaaa
    65941 gaggaaagcg taatatacgc cacctcacga cagtgagctg aaagccgcgt cgcaactg
//
LOCUS       DAZFLD010000003        63299 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.003, whole genome shotgun sequence.
ACCESSION   DAZFLD010000003 DAZFLD010000000
VERSION     DAZFLD010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 63299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 63299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..63299
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.003"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(196..272)
                     /locus_tag="ACHKBL_000136"
     tRNA            complement(196..272)
                     /locus_tag="ACHKBL_000136"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(236..238),aa:Arg,seq:acg)
     gene            complement(276..368)
                     /locus_tag="ACHKBL_000137"
     tRNA            complement(276..368)
                     /locus_tag="ACHKBL_000137"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(332..334),aa:Ser,seq:gct)
     gene            complement(684..869)
                     /gene="csrA"
                     /locus_tag="ACHKBL_000138"
     CDS             complement(684..869)
                     /gene="csrA"
                     /locus_tag="ACHKBL_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HFO5817066.1"
                     /translation="MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHR
                     EEIYQRIQAEKSQQSSY"
     gene            complement(1104..3734)
                     /gene="alaS"
                     /locus_tag="ACHKBL_000139"
     CDS             complement(1104..3734)
                     /gene="alaS"
                     /locus_tag="ACHKBL_000139"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HFO5817067.1"
                     /translation="MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGM
                     NQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDY
                     FKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDN
                     KGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQ
                     FNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDL
                     SNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKL
                     VGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDG
                     ETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMI
                     RVNSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGD
                     KGELKGANFSFAVEDTQKYGQAIGHIGKLAAGSLKVGDAVQADVDEARRARIRLNHSA
                     THLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNL
                     PIETNIMDLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRI
                     ISESGTAAGVRRIEAVTGEGAIATVHADSDRLNEVAHLLKGDSNNLADKVRSVLERTR
                     QLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLG
                     STIIVLATVAEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTD
                     AAALPAALASVKGWVSAKLQ"
     gene            complement(3862..4362)
                     /gene="recX"
                     /locus_tag="ACHKBL_000140"
     CDS             complement(3862..4362)
                     /gene="recX"
                     /locus_tag="ACHKBL_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HFO5817068.1"
                     /translation="MTESTSRRPAYARLLDRAVRILAVRDHSEQELRRKLAAPIMGKN
                     GPEEIDATAEDYERVIAWCHEHGYLDDSRFVARFIASRSRKGYGPARIRQELNQKGIS
                     REATEKAMRECDIDWCALARDQATRKYGEPLPTVFSEKVKIQRFLLYRGYLMEDIQDI
                     WRNFAD"
     gene            complement(4431..5492)
                     /gene="recA"
                     /locus_tag="ACHKBL_000141"
     CDS             complement(4431..5492)
                     /gene="recA"
                     /locus_tag="ACHKBL_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HFO5817069.1"
                     /translation="MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTG
                     SLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDP
                     IYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEI
                     GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKF
                     YASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELV
                     DLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNS
                     TPDFSVDDSEGVAETNEDF"
     gene            complement(5572..6069)
                     /gene="pncC"
                     /locus_tag="ACHKBL_000142"
     CDS             complement(5572..6069)
                     /gene="pncC"
                     /locus_tag="ACHKBL_000142"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HFO5817070.1"
                     /translation="MTDSELMQLSEQVGQSLKARGATVTTAESCTGGWVAKVITDIAG
                     SSAWFERGFVTYSNEAKAQMIGVREETLAQHGAVSEPVVVEMVIGALKAARADYAVSI
                     SGIAGPDGGSEEKPVGTVWFAFATARGEGITRRECFSGDRDAVRRQATAYALQTLWQQ
                     FLQNT"
     gene            complement(6214..7299)
                     /gene="mltB"
                     /locus_tag="ACHKBL_000143"
     CDS             complement(6214..7299)
                     /gene="mltB"
                     /locus_tag="ACHKBL_000143"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HFO5817071.1"
                     /translation="MFKRRYVTLLPLFVLLAACSSKPKPTETETTTGTPSGGFLLEPQ
                     HNVMQMGGDFANNPNAQQFIDKMVNKHGFDRQQLQEILSQAKRLDSVLRLMDNQAPTT
                     SVKPPSGPNGAWLRYRKKFITPDNVQNGVVFWNQYEDALNRAWQVYGVPPEIIVGIIG
                     VETRWGRVMGKTRILDALATLSFNYPRRAEYFSGELETFLLMARDEQDDPLNLKGSFA
                     GAMGYGQFMPSSYKQYAVDFSGDGHINLWDPVDAIGSVANYFKAHGWVKGDQVAVMAN
                     GQAPGLPNGFKTKYSISQLAAAGLTPQQPLGNHQQASLLRLDVGTGYQYWYGLPNFYT
                     ITRYNHSTHYAMAVWQLGQAVALARVQ"
     gene            7555..8118
                     /gene="srlA"
                     /locus_tag="ACHKBL_000144"
                     /gene_synonym="gutA"
     CDS             7555..8118
                     /gene="srlA"
                     /locus_tag="ACHKBL_000144"
                     /gene_synonym="gutA"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HFO5817072.1"
                     /translation="MIETITHGAEWFIGLFQKGGEVFTGMVTGILPLLISLLVIMNAL
                     INFIGQHRIERFAQRCAGNPVSRYLLLPCIGTFVFCNPMTLSLGRFMPEKYKPSYYAA
                     ASYSCHSMNGLFPHINPGELFVYLGIASGLTTLNLPLGPLAVSYLLVGLVTNFFRGWV
                     TDLTTAIFEKKMGIQLEQKVHLAGATS"
     gene            8115..9074
                     /gene="srlE"
                     /locus_tag="ACHKBL_000145"
                     /gene_synonym="gutE"
     CDS             8115..9074
                     /gene="srlE"
                     /locus_tag="ACHKBL_000145"
                     /gene_synonym="gutE"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HFO5817073.1"
                     /translation="MTRIRIEKGTGGWGGPLELEATPGKKIVYITAGTRPAIVDKLAQ
                     LTGWQAIDGFKEGEPAEAEIGVAVIDCGGTLRCGIYPKRRIPTINIHSTGKSGPLAQY
                     IVEDIYVSGVKEENITVVGDATPQPSSVGRDYDTSKKITEQSDGLLAKVGMGMGSAVA
                     VLFQSGRDTIDTVLKTILPFMAFVSALIGIIMASGLGDWIAHGLAPLASHPLGLVMLA
                     LICSFPLLSPFLGPGAVIAQVIGVLIGVQIGLGNIPPHLALPALFAINAQAACDFIPV
                     GLSLAEARQDTVRVGVPSVLVSRFLTGAPTVLIAWFVSGFIYQ"
     gene            9085..9456
                     /gene="srlB"
                     /locus_tag="ACHKBL_000146"
                     /gene_synonym="gutB"
     CDS             9085..9456
                     /gene="srlB"
                     /locus_tag="ACHKBL_000146"
                     /gene_synonym="gutB"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFO5817074.1"
                     /translation="MTVIYQTTITRIGASATDALSDQMLITFREGAPADLEEYCFIHC
                     HGELKGALHPGLQFSLGQHRYPVTAVGSVAEDNLRELGHVTLRFDGLNEAEFPGTVHV
                     AGPVPDDIAPGSVLKFESVKE"
     gene            9460..10239
                     /gene="srlD"
                     /locus_tag="ACHKBL_000147"
     CDS             9460..10239
                     /gene="srlD"
                     /locus_tag="ACHKBL_000147"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HFO5817075.1"
                     /translation="MNQVAVVIGGGQTLGAFLCHGLAAEGYRVAVVDIQSDKAANVAQ
                     EINAEYGEGMAYGFGADATSEQSVLALSRGVDEIFGRVDLLVYSAGIAKAAFISDFQL
                     GDFDRSLQVNLVGYFLCAREFSRLMIRDGIQGRIIQINSKSGKVGSKHNSGYSAAKFG
                     GVGLTQSLALDLAEYGITVHSLMLGNLLKSPMFQSLLPQYATKLGIKPDQVEQYYIDK
                     VPLKRGCDYQDVLNMLLFYASPKASYCTGQSINVTGGQVMF"
     gene            10345..10704
                     /gene="gutM"
                     /locus_tag="ACHKBL_000148"
     CDS             10345..10704
                     /gene="gutM"
                     /locus_tag="ACHKBL_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HFO5817076.1"
                     /translation="MVSALITVAVIAWCAQLALGGWQISRFNRAFDTLCQQGRVGVGR
                     SSGRFKPRVVVAIALDDQQRIVDTLFMKGLTVFARPQKIPAITGMYAGDLQPDVIFPH
                     DPLSQNALSLALKLKRG"
     gene            10771..11544
                     /gene="srlR"
                     /locus_tag="ACHKBL_000149"
                     /gene_synonym="gutR"
     CDS             10771..11544
                     /gene="srlR"
                     /locus_tag="ACHKBL_000149"
                     /gene_synonym="gutR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucitol operon DNA-binding transcriptional
                     repressor SrlR"
                     /protein_id="HFO5817077.1"
                     /translation="MKPRQRQAAILEYLQKQGKCSVEELAQYFDTTGTTIRKDLVILE
                     HAGTVIRTYGGVVLNKEESDPPIDHKTLINTHKKELIAEAAVSFIHDGDSIILDAGST
                     VLQMVPLLSRFNNITVMTNSLHIVNALSELDNEQTILMPGGTFRKKSASFHGQLAENA
                     FEHFTFDKLFMGTDGIDLNAGVTTFNEVYTVSKAMCNAAREVILMADSSKFGRKSPNV
                     VCSLESVDKLITDAGIDPAFRQALEKKGIDVIITGESNE"
     gene            11537..12502
                     /gene="gutQ"
                     /locus_tag="ACHKBL_000150"
     CDS             11537..12502
                     /gene="gutQ"
                     /locus_tag="ACHKBL_000150"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase GutQ"
                     /protein_id="HFO5817078.1"
                     /translation="MSEALLNAGRQTLMLELQEASRLPERLGDDFVRAANIILHCEGK
                     VVVSGIGKSGHIGKKIAATLASTGTPAFFVHPAEALHGDLGMIESRDVMLFISYSGGA
                     KELDLIIPRLEDKSIALLAMTGKPTSPLGLAAKAVLDISVEREACPMHLAPTSSTVNT
                     LMMGDALAMAVMQARGFNEEDFARSHPAGALGARLLNKVHHLMRRDDAIPQVALTASV
                     MDAMLELSRTGLGLVAVCDDQRLVKGVFTDGDLRRWLVGGGALTTPVNEAMTVGGTTL
                     QSQSRAIDAKEILMKRKITAAPVVDENGKLTGAINLQDFYQAGII"
     gene            complement(12499..14013)
                     /gene="norR"
                     /locus_tag="ACHKBL_000151"
     CDS             complement(12499..14013)
                     /gene="norR"
                     /locus_tag="ACHKBL_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HFO5817079.1"
                     /translation="MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALL
                     RYDSRQFIPLAIDGLAKDVLGRRFALEGHPRLEAIARAGDVVRFPADSELPDPYDGLI
                     PGQESLKVHACVGLPLFAGQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNA
                     LLIEQLESQNMLPGDAAPFEAVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETG
                     TGKELVAKAIHEASPRAVNPLVYLNCAALPESVAESELFGHVKGAFTGAISNRSGKFE
                     MADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRSLRVDVRVLAATNRDLREE
                     VLAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRLGLSRVVLSAGAR
                     NLLQHYSFPGNVRELEHAIHRAVVLSRATRSGDEVILEAQHFAFPEVTLPPPEAAAVP
                     IVKQNLREATEAFQRETIRQALAQNHHNWAACARMLETDVANLHRLAKRLGLKD"
     gene            14200..15639
                     /gene="norV"
                     /locus_tag="ACHKBL_000152"
     CDS             14200..15639
                     /gene="norV"
                     /locus_tag="ACHKBL_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016966 - nitric oxide reductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HFO5817080.1"
                     /translation="MSIVVKNNIHWVGQRDWEVRDFHGTEYKTLRGSSYNSYLIREEK
                     NVLIDTVDHKFSREFVQNLRNEIDLTDIDYIVINHAEEDHAGALTELMAQIPDTPIYC
                     TANAIDSINGHHHHPEWNFNVVKTGDTLDIGNGKQLIFVETPMLHWPDSMMTYLTGDA
                     VLFSNDAFGQHYCDEHLFNDEVDQTELFEQCQRYYANILTPFSRLVTPKITEILGFNL
                     PVDMIATSHGVVWRDNPTQIVELYLKWAADYQEDRITIFYDTMSNNTRMMADAIAQGI
                     AETDPRVAVKIFNVARSDKNEILTNVFRSKGVLVGTSTMNNVMMPKIAGLVEEMTGLR
                     FRNKRASAFGSHGWSGGAVDRLSTRLQDAGFEMSLSLKAKWRPDQDALELCREHGREI
                     ARQWALAPLPQSTVNTVVKEETSATTTADLGPRMQCSVCQWIYDPAKGEPMQDVAPGT
                     PWSEVPDNFLCPECSLGKDVFDELASEAK"
     gene            15636..16769
                     /gene="norW"
                     /locus_tag="ACHKBL_000153"
     CDS             15636..16769
                     /gene="norW"
                     /locus_tag="ACHKBL_000153"
                     /EC_number="1.18.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016731 - oxidoreductase activity, acting
                     on iron-sulfur proteins as donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavorubredoxin reductase NorW"
                     /protein_id="HFO5817081.1"
                     /translation="MSNGIVIIGSGFAARQLVKNIRKQDACIPLTLIAADSMDEYNKP
                     DLSHVISQRQRADDLTRQTAGEFAEQFNLHLFPHTWVTDIDAEAHVVKSQNNQWQYDK
                     LVLATGASAFVPPVPGRELMLTLNSQQEYRACETQLRNARRVLIVGGGLIGSELAMDF
                     CRAGKAVTLIDNAASILAALMPPEVSSRLQHRLTEMGVHLLLKSQLQGLEKTDSGILA
                     TLDRQRSIEVDAVIAATGLRPETALARRAGLTINRGVCVDSYLQTSNADIYALGDCAE
                     INGQVLPFLQPIQLSAMVLAKNLLGNNTPLKLPAMLVKIKTPELPLHLAGETQRQDLR
                     WQINTERQGMVARGVDDADQLRAFVVSEDRMKEAFGLLKTLPV"
     gene            complement(16993..19245)
                     /gene="hypF"
                     /locus_tag="ACHKBL_000154"
     CDS             complement(16993..19245)
                     /gene="hypF"
                     /locus_tag="ACHKBL_000154"
                     /EC_number="6.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016743 - carboxyl- or
                     carbamoyltransferase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HFO5817082.1"
                     /translation="MAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGD
                     GVEVRLLEDPETFLVQLHQHCPPLARIDSVEREPFIWSQLPTEFTIRQSAGGVMNTQI
                     VPDAATCPACLAEMNTPGERRWRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAFPLCP
                     ACDKEYRDPLDRRFHAQPVACPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGNIVAI
                     KGIGGFHLACDARNSNAVATLRARKHRPAKPLAVMLPVAEGLPDAARQLLTTPAAPIV
                     LVDKKYVPELCDDIAPGLNEVGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISN
                     EQALADLQGIADGFLIHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALALPPGFKNVP
                     PVLCLGADLKNTFCLVRGEQAVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPQYVV
                     HDAHPGYVSSQWAREMNLPTQTVLHHHAHAAACLAEHHWPLDGGDVIALTLDGIGMGE
                     NGALWGGECLRVNYRECQHLGGLPAVALAGGDLAAKQPWRNLLAQCLRFVPEWQNYPE
                     TASVQQQNWSVLARAIERGINAPLASSCGRLFDAVAAGLGCAPATLSYEGEAACALEA
                     LAASCDGVTHPVTMPLVDNQLDLATFWQQWLSWQAPVNQRAWAFHDALAQGFAALMRE
                     QATMRGITTLVFSGGVIHNRLLRARLAHYLADFTLLFPQSLPAGDGGLSLGQGVIAAA
                     RWLAGEVQNG"
     gene            complement(19398..19925)
                     /gene="hydN"
                     /locus_tag="ACHKBL_000155"
     CDS             complement(19398..19925)
                     /gene="hydN"
                     /locus_tag="ACHKBL_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport protein HydN"
                     /protein_id="HFO5817083.1"
                     /translation="MNRFIIADASKCIGCRTCEVACVVSHQENQDCASLTPETFLPRI
                     HVIKGVNISTATVCRQCEDAPCANVCPNGAISRDKGFVHVMQERCIGCKTCVVACPYG
                     AMEVVVRPVIRNSGAGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
                     EQLSAEKRRRTALMF"
     gene            complement(20074..21084)
                     /gene="ascG"
                     /locus_tag="ACHKBL_000156"
     CDS             complement(20074..21084)
                     /gene="ascG"
                     /locus_tag="ACHKBL_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator AscG"
                     /protein_id="HFO5817084.1"
                     /translation="MTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESG
                     YRPNLLARNLSAKSTQTLGLVVTNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKHS
                     AEEERQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRKNSSHSVW
                     CDHKQTSFNAVAELINAGHQEIAFLTGSMDSPTSVERLAGYKDALAQHGIALNEKLIA
                     NGKWTPASGAEGVEMLLERGAKFSALVASNDDMAIGAMKALHERGVAVPEQVSVIGFD
                     DIAIAPYTVPALSSVKIPVTEMIQEIIGRLIFMLDGGAYTPPKTFSGKLIRRDSLIAL
                     SR"
     gene            21344..22801
                     /gene="ascF"
                     /locus_tag="ACHKBL_000157"
     CDS             21344..22801
                     /gene="ascF"
                     /locus_tag="ACHKBL_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose/arbutin/salicin transporter
                     subunit IIBC"
                     /protein_id="HFO5817085.1"
                     /translation="MAKNYAALARSVIAALGGVDNISAVTHCMTRLRFVIKDDQLIDS
                     PTLKTISGVLGVVRSDNQCQVIIGNTVSQAFQEVVSLLPGDMQPAQPVGKPKLTLRRI
                     GAGILDALIGTMSPLIPAIIGGSMVKLLAMILEMSGVLTKGSPTLTILNVIGDGAFFF
                     LPLMVAASAAIKFKTNMSLAIAIAGVLVHPSFIELMAKAAQGEHVEFALIPVTAVKYT
                     YTVIPALVMTWCLSYIERWVDRITPAVTKNFLKPMLIVLIAAPLAILLIGPIGIWIGS
                     AISALVYTIHGYLGWLSVAIMGALWPLLVMTGMHRVFTPTIIQTIAETGKEGMVMPSE
                     IGANLSLGGSSLAVAWKTKNPELRQTALAAAASAIMAGISEPALYGVAIRLKRPLIAS
                     LISGFICGAVAGMAGLASHSMAAPGLFTSVQFFDPANPMSIVWVFAVMALAVVLSFIL
                     TLLLGFEDIPVEEATAEARKHQSAQPTVAKEVSLN"
     gene            22810..24234
                     /gene="ascB"
                     /locus_tag="ACHKBL_000158"
     CDS             22810..24234
                     /gene="ascB"
                     /locus_tag="ACHKBL_000158"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase AscB"
                     /protein_id="HFO5817086.1"
                     /translation="MSVFPEGFLWGGALAANQSEGAYREGGKGLTTVDMIPHGEHRMA
                     VKLGLEKRFQLRDDEFYPSHEATDFYHRYKEDIALMAEMGFKVFRTSIAWSRLFPQGD
                     ELTPNQQGIAFYRSVFEECKKYGIEPLVTLCHFDVPMHLVTEYGSWRNRKLVEFFSRY
                     ARTCFEAFDGLVKYWLTFNEINIMLHSPFSGAGLVFEEGENQDQVKYQAAHHQLIASA
                     LATKIAHEVNPQNQVGCMLAGGNFYPYSCKPEDVWAALEKDRENLFFIDVQARGAYPA
                     YSARVFREKGVTIDKAPGDDEILKNTVDFVSFSYYASRCASAEMNANNSSAANVVKSL
                     RNPYLQVSDWGWGIDPLGLRITMNMMYDRYQKPLFLVENGLGAKDELAANGEINDDYR
                     ISYLREHIRAMGEAIADGIPLMGYTTWGCIDLVSASTGEMSKRYGFVYVDRDDAGNGT
                     LARTRKKSFWWYKKVIASNGEDLE"
     gene            complement(24392..24862)
                     /gene="hycI"
                     /locus_tag="ACHKBL_000159"
     CDS             complement(24392..24862)
                     /gene="hycI"
                     /locus_tag="ACHKBL_000159"
                     /EC_number="3.4.23.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation peptidase HycI"
                     /protein_id="HFO5817087.1"
                     /translation="MTDVLLCVGNSMMGDDGAGPLLAEKCAAAPKGNWVVIDGGSAPE
                     NDIVAIRELRPTRLLIVDATDMGLNPGEIRIIDPDDIAEMFMMTTHNMPLNYLIDQLK
                     EDIGEVIFLGIQPDIVGFYYPMTQPIKDAVETVYQRLEGWEGNGGFAQLAVEEE"
     gene            complement(24855..25265)
                     /gene="hycH"
                     /locus_tag="ACHKBL_000160"
     CDS             complement(24855..25265)
                     /gene="hycH"
                     /locus_tag="ACHKBL_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase assembly protein HycH"
                     /protein_id="HFO5817088.1"
                     /translation="MSEKVVFSQLSRKFIDENDATPAEAQQVVYYSLAIGHHLGVIDC
                     LEAALTCPWDDYLAWIATLEAGSEARRKMEGVPKYGEIVIDINHVPMLANAFDKARAA
                     QTSQQKEWSTMLLSMLHDIHQENAIYLMVRRLRD"
     gene            complement(25262..26029)
                     /gene="hycG"
                     /locus_tag="ACHKBL_000161"
     CDS             complement(25262..26029)
                     /gene="hycG"
                     /locus_tag="ACHKBL_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycG"
                     /protein_id="HFO5817089.1"
                     /translation="MSNLLGPRDANGIPVPMTVDESIASMKASLLKKIKRSAYVYRVD
                     CGGCNGCEIEIFATLSPLFDAERFGIKVVPSPRHADILLFTGAVTRAMRSPALRAWQS
                     APDPKICISYGACGNSGGIFHDLYCVWGGTDKIVPVDVYIPGCPPTPAATLYGFAMAL
                     GLLEQKIHARGPGEQDEQPAEILHGDMVQPLRVKVDREARRLAGYRYGRQIADDYLTQ
                     LGQGEEQVARWLEAENDPRLNEIVSHLNHVVEEARIR"
     gene            complement(26029..26571)
                     /gene="hycF"
                     /locus_tag="ACHKBL_000162"
     CDS             complement(26029..26571)
                     /gene="hycF"
                     /locus_tag="ACHKBL_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycF"
                     /protein_id="HFO5817090.1"
                     /translation="MFTFIKKVIKTGTATSSYPLEPIAVDKNFRGKPEQNPQQCIGCA
                     ACVNACPSNALTVETDLATGELAWEFNLGRCIFCGRCEEVCPTAAIKLSQEYELAVWK
                     KEDFLQQSRFALCNCRVCNRPFAVQKEIDYAIALLKHNGDSRAENHRESFETCPECKR
                     QKCLVPSDRIELTRHMKEAI"
     gene            complement(26581..28290)
                     /gene="hycE"
                     /locus_tag="ACHKBL_000163"
     CDS             complement(26581..28290)
                     /gene="hycE"
                     /locus_tag="ACHKBL_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycE"
                     /protein_id="HFO5817091.1"
                     /translation="MSEEKLGQHYLAALNEAFPGVVLDHAWQTKDQLTVTVKVNYLPE
                     VVEFLYYKQGGWLSVLFGNDERKLNGHYAVYYVLSMEKGTKCWVTVRVEVDANKPEYP
                     SVTPRVPAAVWGEREVRDMYGLIPVGLPDERRLVLPDDWPDELYPLRKDSMDYRQRPA
                     PTTDAETYEFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHR
                     GMEKLAETRMGYNEVTFLSDRVCGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILL
                     EVERLHSHLLNLGLACHFTGFDSGFMQFFRVRETSMKMAEILTGARKTYGLNLIGGIR
                     RDLLKDDMIQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEIARDFSNVGP
                     MVRASGHARDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLD
                     NLPGGPLMVEGFTYIPHRFALGFAEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPT
                     LRYMLRGNTVSDAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYSIERKNS
                     PLK"
     gene            complement(28308..29231)
                     /gene="hycD"
                     /locus_tag="ACHKBL_000164"
     CDS             complement(28308..29231)
                     /gene="hycD"
                     /locus_tag="ACHKBL_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycD"
                     /protein_id="HFO5817092.1"
                     /translation="MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYR
                     DIIKLLGRQSVGPDASGWVFRLTPYVMVGVMLTIATALPVVTVGSPLPQLGDLITLLY
                     LFAIARFFFAISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNI
                     TDTVYHWPLSQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFG
                     VMKWGISLKQLVVLQMFVGVFIPWGQMETFTVGGLLLALVIAIVKLVVGVLVIALFEN
                     SMARLRLDITPRITWAGFGFAFLAFVSLLAA"
     gene            complement(29234..31060)
                     /gene="hycC"
                     /locus_tag="ACHKBL_000165"
     CDS             complement(29234..31060)
                     /gene="hycC"
                     /locus_tag="ACHKBL_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit 3"
                     /protein_id="HFO5817093.1"
                     /translation="MSAISLINSGVAWFVAAAVLAFLFSFQKALSGWIAGIGGAVGSL
                     YTAAAGFTVLTGAVGVSGALSLVSYDVQISPLNAIWLITLGLCGLFVSLYNIDWHRHA
                     QVKCNGLQINMLMAAAVCAVIASNLGMFVVMAEIMALCAVFLTSNSKEGKLWFALGRL
                     GTLLLAIACWLLWQRYGTLDLRLLDMRMQQLPLGSDIWLLGVIGFGLLAGIIPLHGWV
                     PQAHANASAPAAALFSTVVMKIGLLGILTLSLLGGNAPLWWGIALLVLGMITAFVGGL
                     YALMEHNIQRLLAYHTLENIGIILLGLGAGVTGIALEQPALIALGLVGGLYHLLNHSL
                     FKSVLFLGAGSVWFRTGHRDIEKLGGIGKKMPVISIAMLVGLMAMAALPPLNGFAGEW
                     VIYQSFFKLSNSGAFVARLLGPLLAVGLAITGALAVMCMAKVYGVTFLGAPRTKEAEN
                     ATCAPLLMSVSVVALAICCVIGGVAAPWLLPMLSAAVPLPLEPANTTVSQPMITLLLI
                     ACPLLPFIIMAICKGDRLPSRSRGAAWVCGYDHEKSMVITAHGFAMPVKQAFAPVLKL
                     RKWLNPVSLVPGWQCEGSALLFRRMALVELAVLVVIIVSRGA"
     gene            complement(31057..31668)
                     /gene="hycB"
                     /locus_tag="ACHKBL_000166"
     CDS             complement(31057..31668)
                     /gene="hycB"
                     /locus_tag="ACHKBL_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycB"
                     /protein_id="HFO5817094.1"
                     /translation="MNRFVIADSTLCIGCHTCEAACSETHRQHGLQSMPRLRVMLNEK
                     ESAPQLCHHCEDAPCAVVCPVNAITRVDGAVQLNESLCVSCKLCGIACPFGAIEFSGS
                     RPLDIPANANTPKAPPAPPAPARVSTLLDWVPGIRAIAVKCDLCSFDEQGPACVRMCP
                     TKALHLVDNTDIARVSKRKRELTFNTDFGDLTLFQQAQSGEAK"
     gene            complement(31793..32254)
                     /gene="hycA"
                     /locus_tag="ACHKBL_000167"
     CDS             complement(31793..32254)
                     /gene="hycA"
                     /locus_tag="ACHKBL_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase regulator HycA"
                     /protein_id="HFO5817095.1"
                     /translation="MTIWEISEKADYIAQRHRRLQDQWHIYCNSLVQGITLSKARLHH
                     AMSCAPDKELCFVLFEHFRIYVTLADGFNSHTIEYYVETKDGEDKQRIAQAQLSIDGM
                     IDGKVNIRDREQVLEHYLEKIAGVYDSLYTAIENNVPVNLSQLVKGQSPAA"
     gene            32466..32816
                     /gene="hypA"
                     /locus_tag="ACHKBL_000168"
     CDS             32466..32816
                     /gene="hypA"
                     /locus_tag="ACHKBL_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HFO5817096.1"
                     /translation="MHEITLCQRALELIEQQAAKHGAKRVTGVWLKIGAFSCVETSSL
                     AFCFDLVCRGSVAEGCKLHLEEQEAECWCETCQQYVTLLTQRVRRCPQCHGDMLQIVA
                     DDGLQIRRIEIDQE"
     gene            32820..33692
                     /gene="hypB"
                     /locus_tag="ACHKBL_000169"
     CDS             32820..33692
                     /gene="hypB"
                     /locus_tag="ACHKBL_000169"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HFO5817097.1"
                     /translation="MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPKMKITGIK
                     APEFTPSQTEEGDLHYGHGEAGTHAPGMSQRRMLEVEIDVLDKNNRLAERNRARFAAR
                     KQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQV
                     NTGKGCHLDAQMIADAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEG
                     EDKPLKYPHMFAAASLMLLNKVDLLPYLNFDVEKCIACAREVNPEIEIILISATSGEG
                     MDQWLNWLETQRCA"
     gene            33683..33955
                     /gene="hypC"
                     /locus_tag="ACHKBL_000170"
     CDS             33683..33955
                     /gene="hypC"
                     /locus_tag="ACHKBL_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation protein HypC"
                     /protein_id="HFO5817098.1"
                     /translation="MCIGVPGQIRTIDGNQAKVDVCGIQRDVDLTLVGSCDENGQPRV
                     GQWVLVHVGFAMSVINEAEARDTLDALQNMFDVEPDVGALLYGEEK"
     gene            33955..35076
                     /gene="hypD"
                     /locus_tag="ACHKBL_000171"
     CDS             33955..35076
                     /gene="hypD"
                     /locus_tag="ACHKBL_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HFO5817099.1"
                     /translation="MRFVDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGH
                     THAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRIDTCVEIASHPEVIFCTFGDAMRV
                     PGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQ
                     AKARDVQNFYFFCQHITLIPTLRSLLEQPDNGIDAFLAPGHVSMVIGTDAYNFIASDF
                     HRPLVVAGFEPLDLLQGVVMLVEQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCV
                     NGDSEWRGLGVIESSGVHLTPDYQRFDAETHFRPAPQQVCDDPRARCGEVLTGKCKPH
                     QCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQESEA"
     gene            35073..36083
                     /gene="hypE"
                     /locus_tag="ACHKBL_000172"
     CDS             35073..36083
                     /gene="hypE"
                     /locus_tag="ACHKBL_000172"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417210.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation carbamoyl dehydratase
                     HypE"
                     /protein_id="HFO5817100.1"
                     /translation="MNNIQLAHGSGGQAMQQLINSLFMEAFANPWLAEQEDQARLDLA
                     QLVAEGDRLAFSTDSYVIDPLFFPGGNIGKLAICGTANDVAVSGAIPRYLSCGFILEE
                     GLPMETLKAVVTSMAETARAAGIAIVTGDTKVVQRGAADKLFINTAGMGAIPANIHWG
                     AQTLTAGDVLLVSGTLGDHGATILNLREQLGLDGELVSDCAVLTPLIQTLRDIPGVKA
                     LRDATRGGVNAVVHEFAAACGCGIELSEAALPVKPAVRGVCELLGLDALNFANEGKLV
                     IAVERNAAEQVLAALHSHPLGKDAALIGEVVERKGVRLAGLYGVKRTLDLPHAEPLPR
                     IC"
     gene            36157..38235
                     /gene="flhA"
                     /locus_tag="ACHKBL_000173"
     CDS             36157..38235
                     /gene="flhA"
                     /locus_tag="ACHKBL_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase transcriptional activator
                     FlhA"
                     /protein_id="HFO5817101.1"
                     /translation="MSYTPMSDLGQQGLFDITRTLLQQPDLASLCEALSQLVKRSALA
                     DNAAIVLWQAQTQRASYYASREKDTPIKYEDETVLAHGPVRSILSRPDTLHCSYEEFC
                     ETWPQLATGGLYPKFGHYCLMPLAAEGHIFGGCEFIRHDDRPWSEKEFNRLQTFTQIV
                     SVVTEQIQSRVVNNVDYELLCRERDNFRILVAITNAVLSRLDMDELVSEVAKEIHYYF
                     DIDDISIVLRSHRKNKLNIYSTHYLDKQHPAHEQSEVDEAGTLTERVFKSKEMLLINL
                     HERDDLAPYERMLFNTWGNQIQTLCLLPLMSGDTMLGVLKLAQCEEKVFTTTNLSLLR
                     QIAERVAIAVDNALAYQEIHRLKERLVDENLALTEQLNNVDSEFGEIIGRSEAMYSVL
                     KQVEMVAQSDSTVLILGETGTGKELIARAIHNLSGRNNRRMVKMNCAAMPAGLLESDL
                     FGHERGAFTGASAQRIGRFELADKSSLFLDEVGDMPLELQPKLLRVLQEQEFERLGSN
                     KIIQTDVRLIAATNRDLKKMVADREFRSDLYYRLNVFPIHLPPLRERPEDIPLLAKAF
                     TFKIARRLGRNIDSIPAETLRILSNMEWPGNVRELENVIERAVLLTRGNVLQLSLPDI
                     ALPEPETPPAATVVAQEGEDEYQLIVRVLKETNGVVAGPKGAAQRLGLKRTTLLSRMK
                     RLGIDKSALI"
     gene            38280..38708
                     /locus_tag="ACHKBL_000174"
     CDS             38280..38708
                     /locus_tag="ACHKBL_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012134844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HFO5817102.1"
                     /translation="MAVSARNQLTGTVSTVAMGAVNDEVELTLAGGAKLVAIVTHSSQ
                     QALGLAKGKEAIALIKAPWVTLATEDCGLKFSARNQFAGSVSTITEGAVNATVHIKTD
                     AGFEIVAVVTNESQDEMKLTTGSRVIALIKASAILIATKA"
     gene            complement(38747..39091)
                     /gene="ygbA"
                     /locus_tag="ACHKBL_000175"
     CDS             complement(38747..39091)
                     /gene="ygbA"
                     /locus_tag="ACHKBL_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HFO5817103.1"
                     /translation="MSGKRISREKLTIKKMIDLYQAKCPQASAEPEHYEALFAYAQKR
                     LDKCVFGEEKPACKQCPVHCYQPAKREEMKQIMRWAGPRMLWRHPILTVRHLIDDKRP
                     VPELPEKYRPKK"
     gene            39377..41938
                     /gene="mutS"
                     /locus_tag="ACHKBL_000176"
     CDS             39377..41938
                     /gene="mutS"
                     /locus_tag="ACHKBL_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFO5817104.1"
                     /translation="MRTIENFDAHTPMMQQYLKLKAQHPEILLFYRMGDFYELFYDDA
                     KRASQLLDISLTKRGASAGEPIPMAGIPYHAVENYLAKLVNQGESVAICEQIGDPATS
                     KGPVERKVVRIVTPGTISDEALLQERQDNLLAAIWQDSKGFGYATLDISSGRFRLSEP
                     ADRETMAAELQRTNPAELLYAEDFAEMSLIEGRRGLRRRPLWEFEIDTARQQLNLQFG
                     TRDLVGFGVENAPRGLCAAGCLLQYAKDTQRTTLPHIRSITMERQQDSIIMDAATRRN
                     LEITQNLAGGAENTLASVLDCTVTPMGSRMLKRWLHMPVRDTRVLLERQQTIGALQDF
                     TAELQPVLRQVGDLERILARLALRTARPRDLARMRHAFQQLPELRAQLEGVDSAPVQA
                     LREKMGEFAELRDLLERAIIDTPPVLVRDGGVIATGYNEELDEWRALADGATDYLERL
                     EVRERERTGLDTLKVGFNAVHGYYIQISRGQSHLAPINYMRRQTLKNAERYIIPELKE
                     YEDKVLTSKGKALALEKQLYEELFDLLLPHLEALQQSASALAELDVLVNLAERAYTLN
                     YTCPTFIDKPGIRITEGRHPVVEQVLNEPFIANPLNLSPQRRMLIITGPNMGGKSTYM
                     RQTALIALMAYIGSYVPAQKVEIGPIDRIFTRVGAADDLASGRSTFMVEMTETANILH
                     NATEYSLVLMDEIGRGTSTYDGLSLAWACAENLANKIKALTLFATHYFELTQLPEKME
                     GVANVHLDALEHGDTIAFMHSVQDGAASKSYGLAVAALAGVPKEVIKRARQKLRELES
                     ISPNAAATQVDGTQMSLLSVPEETSPAVEALENLDPDSLTPRQALEWIYRLKSLV"
     gene            42044..42700
                     /gene="pphB"
                     /locus_tag="ACHKBL_000177"
     CDS             42044..42700
                     /gene="pphB"
                     /locus_tag="ACHKBL_000177"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HFO5817105.1"
                     /translation="MPSTRYQKINAHHYHHIWAVGDIHGDYQLLQSRLHQLSFCPETD
                     LLISVGDNIDRGPESLNVLRLLNQPWFISVKGNHEAMALEAFETGDGNMWLASGGDWF
                     FDLNDSEQQEAIDLLLKFHHLPHIIEITNDTIKYVIAHADYPGKEYQFGKEIAESELL
                     WPVDRVQKSLNSELQKINGADYFIFGHMMFDNIQTFANQIYIDTGSPKSGRLSFYKIK
                     "
     gene            complement(42751..43518)
                     /gene="ygbI"
                     /locus_tag="ACHKBL_000178"
     CDS             complement(42751..43518)
                     /gene="ygbI"
                     /locus_tag="ACHKBL_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor YgbI"
                     /protein_id="HFO5817106.1"
                     /translation="MIPVERRQIILEMVAEKGIVSIAELTDRMNVSHMTIRRDLQKLE
                     QQGAVVLVSGGVQSPGRVAHEPSHQVKTALAMTQKAAIGKLAASLVQPGRCIYLDAGT
                     TTLAIAQHLTHMEPLTVVTNDFVIADYLLDNSNCTIIHTGGAVCRENRSCVGEAAATM
                     LRSLMIDQAFISASSWSVRGISTPAEDKVTVKRAIASASRQRVLVCDATKYGQVATWL
                     ALPLSEFDQIITDDGLPESASRALAKLDLSLLVAKNE"
     gene            43714..44622
                     /gene="ltnD"
                     /locus_tag="ACHKBL_000179"
     CDS             43714..44622
                     /gene="ltnD"
                     /locus_tag="ACHKBL_000179"
                     /EC_number="1.1.1.411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonate dehydrogenase"
                     /protein_id="HFO5817107.1"
                     /translation="MKTGSEFHVGIVGLGSMGMGAALSCVRAGLSTWGADLNSNACAT
                     LKEAGACGVSDNAATFAEKLDALLVLVVNAAQVKQVLFGETGVAQHLKPGTAVMVSST
                     IASADAQEIATALAGFDLEMLDAPVSGGAVKAANGEMTVMASGSDIAFERLAPVLEAV
                     AGKVYRIGAEPGLGSTVKIIHQLLAGVHIAAGAEAMALAARAGIPLDVMYDVVTNAAG
                     NSWMFENRMRHVVDGDYTPHSAVDIFVKDLGLVADTAKALHFPLPLASTALNMFTSAS
                     NAGYGKEDDSAVIKIFSGITLPGAKS"
     gene            44619..45881
                     /gene="otnK"
                     /locus_tag="ACHKBL_000180"
     CDS             44619..45881
                     /gene="otnK"
                     /locus_tag="ACHKBL_000180"
                     /EC_number="2.7.1.217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxo-tetronate kinase"
                     /protein_id="HFO5817108.1"
                     /translation="MIKIGVIADDFTGATDIASFLVENGLPTVQINGVPTGKMPEAID
                     ALVISLKTRSCPVVEATQQSLAALSWLQQQGCKQIYFKYCSTFDSSAKGNIGPVTDAL
                     MDALDTPFTVFSPALPVNGRTVYQGYLFVMNQLLAESGMRHHPVNPMTDSYLPRLVEA
                     QSTGRCGVVSAHVFEQGVDAVRQELARLQQEGYRYAVLDALTEHHLEIQGEALRDTPL
                     VTGGSGLAIGLARQWAQENGNQAREAGRPLAGRGVVLSGSCSQMTNRQVAHYRQIAPA
                     REVDVARCLSTETLAAYAHELAEWVLGQESVLAPLVFATASTDALAAIQQQYGAQKAS
                     QAVETLFSQLAARLAAEGVTRFIVAGGETSGVVTQSLGIKGFHIGPTISPGVPWVNAL
                     DKPVSLALKSGNFGDEAFFSRAQREFLS"
     gene            45878..46516
                     /gene="ygbL"
                     /locus_tag="ACHKBL_000181"
     CDS             45878..46516
                     /gene="ygbL"
                     /locus_tag="ACHKBL_000181"
                     /EC_number="4.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HFO5817109.1"
                     /translation="MSDFAKVEQSLREEMTRIASSFFQRGYATGSAGNLSLLLPDGNL
                     LATPTGSCLGNLDPQRLSKVTADGEWLSGDKPSKEVLFHLALYRNNPRCKAVVHLHST
                     WSTALSCLEGLDSNNVIRPFTPYVVMRMGNVPLVPYYRPGDKRIAQDLAELAADNQAF
                     LLANHGPVVCGESLQEAANNMEELEETAKLIFILGDRPIRYLTAGEIAELRS"
     gene            46521..47297
                     /gene="ygbM"
                     /locus_tag="ACHKBL_000182"
     CDS             46521..47297
                     /gene="ygbM"
                     /locus_tag="ACHKBL_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HFO5817110.1"
                     /translation="MPRFAANLSMMFTEVPFIERFAAARKAGFDAVEFLFPYDYSPQQ
                     IQQQLAQNHLTLALFNTAPGDINAGEWGLSALPGREHEARADIDLALEYALALNCEQV
                     HVMAGVVPAGEDAERYRAVFIDNLRYAADHFAPHGKRILVEALSPGVKPHYLFSSQYQ
                     ALAIVEEVARDNVFIQLDTFHAQKVDGNLTHLIRDYAGKYAHVQIAGLPDRHEPDDGE
                     INYPWLFRLFDEVGYQGWIGCEYKPRGLTEEGLGWFDAWR"
     gene            47386..48750
                     /gene="ygbN"
                     /locus_tag="ACHKBL_000183"
     CDS             47386..48750
                     /gene="ygbN"
                     /locus_tag="ACHKBL_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015128 - gluconate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035429 - gluconate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family transporter"
                     /protein_id="HFO5817111.1"
                     /translation="MSTITLLCIALTGVIMLLLLVIKAKVQPFVALLLVSLLVALAAG
                     IPAGEVGKVMIAGMGGVLGSVTIIIGLGAMLGRMIEHSGGAESLANYFSRKLGDKRTI
                     AALTLAAFFLGIPVFFDVGFIILAPIIYGFAKVAKISPLKFGLPVAGIMLTVHVAVPP
                     HPGPVAAAGLLHADIGWLTIIGIAISIPVGIVGYFAAKIINKRQYAMSVEVLEQMQLA
                     PASEEGATKLSDKINPPGVALVTSLIVIPIAIIMAGTVSATLMPPSHPLLGTLQLIGS
                     PMVALMIALVLAFWLLALRRGWSLQHTSDIMGSALPTAAVVILVTGAGGVFGKVLVES
                     GVGKALANMLQMIDLPLLPAAFIISLALRASQGSATVAILTTGGLLSEAVMGLNPIQC
                     VLVTLAACFGGLGASHINDSGFWIVTKYLGLSVADGLKTWTVLTTILGFTGFLITWCI
                     WAVI"
     gene            complement(48788..49024)
                     /locus_tag="ACHKBL_000184"
     CDS             complement(48788..49024)
                     /locus_tag="ACHKBL_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-oxidative hydroxyarylic acid decarboxylases
                     subunit D"
                     /protein_id="HFO5817112.1"
                     /translation="MICPRCADEQIEVMAKSPVKDVWMVYQCQHCLYTWRDTEPLRRT
                     SREHYPEAFRMTQKDIDDAPMVPSIPPLLAEGKR"
     gene            complement(49035..50462)
                     /locus_tag="ACHKBL_000185"
     CDS             complement(49035..50462)
                     /locus_tag="ACHKBL_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-oxidative hydroxyarylic acid decarboxylases
                     subunit C"
                     /protein_id="HFO5817113.1"
                     /translation="MAFDDLRSFLQALDDHGQLLKISEEVNAEPDLAAAANATGRIGD
                     GAPALWFDNIRGFIDARVAMNTIGSWQNHAISLGLPPNTPVKKQIDEFIRRWDNFPIA
                     PERRANPAWAQNTVDGDEINLFNILPLFRLNDGDGGFYLDKACVVSRDPLDPDNFGKQ
                     NVGIYRMEVKGKRKLGLQPVPMHDIALHLHKAEERGEDLPIAITLGNDPIITLMGATP
                     LKYDQSEYEMAGALRESPYPIATAPLTGFDVPWGSEVILEGVIESRKREIEGPFGEFT
                     GHYSGGRNMTVVRIDKVSYRTKPIFESLYLGMPWTEIDYLMGPATCVPLYQQLKAEFP
                     EVQAVNAMYTHGLLAIISTKKRYGGFARAVGLRAMTTPHGLGYVKMVIMVDEDVDPFN
                     LPQVMWALSSKVNPAGDLVQLPNMSVLELDPGSSPAGITDKLIIDATTPVAPDNRGHY
                     SQPVVDLPETKAWAEKLTAMLAARQ"
     gene            complement(50462..51055)
                     /locus_tag="ACHKBL_000186"
     CDS             complement(50462..51055)
                     /locus_tag="ACHKBL_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-oxidative hydroxyarylic acid decarboxylases
                     subunit B"
                     /protein_id="HFO5817114.1"
                     /translation="MKLIVGMTGATGAPLGVALLQALREMPNVETHLVMSKWAKTTIE
                     LETPYSARDVAALADFSHNPADQAATISSGSFRTDGMIVIPCSMKTLAGIRAGYADGL
                     VGRAADVVLKEGRKLVLVPREMPLSTIHLENMLALSRMGVAMVPPMPAFYNHPETVDD
                     IVHHVVARVLDQFGLEHPHARRWQGLPQARNFSQENE"
     gene            51202..51609
                     /locus_tag="ACHKBL_000187"
     CDS             51202..51609
                     /locus_tag="ACHKBL_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001208086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFO5817115.1"
                     /translation="MALRNKAFHQLRQLFQQHTARWQHELPDLTKPQYAVMRAIADKP
                     GIEQVALIEAAVSTKATLAEMLARMENRGLVRREHDAADKRRRFVWLTAEGEKVLAAA
                     IPIGDSVDEEFLGRLSAEEQELFMQLVRKMMSK"
     gene            complement(51730..52722)
                     /gene="rpoS"
                     /locus_tag="ACHKBL_000188"
     CDS             complement(51730..52722)
                     /gene="rpoS"
                     /locus_tag="ACHKBL_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HFO5817116.1"
                     /translation="MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEE
                     LLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLV
                     VKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
                     QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNER
                     ITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLAR
                     RFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQGLNIEALFRE"
     gene            complement(52785..53879)
                     /gene="nlpD"
                     /locus_tag="ACHKBL_000189"
     CDS             complement(52785..53879)
                     /gene="nlpD"
                     /locus_tag="ACHKBL_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator NlpD"
                     /protein_id="HFO5817117.1"
                     /translation="MSLVSLWLAGCSDTSNPPAPVSSVNGNAPANTNSGMLITPPPKM
                     GTTSTAQQPQIQPVQQPQIQATQQPQIQPVQPVAQQPVQMENGRIVYNRQYGNIPKGS
                     YSGSTYTVKKGDTLFYIAWITGNDFRDLAQRNNIQAPYALNVGQTLQVGNASGTPITG
                     GNAITQADAAEQGVVIKPAQNSTVAVASQPTITYSESSGEQSANKMLPNNKPTATTVT
                     APVTVPTASTTEPTVSSTSTSTPISTWRWPTEGKVIETFGASEGGNKGIDIAGSKGQA
                     IIATADGRVVYAGNALRGYGNLIIIKHNDDYLSAYAHNDTMLVREQQEVKAGQKIATM
                     GSTGTSSTRLHFEIRYKGKSVNPLRYLPQR"
     gene            complement(54064..54690)
                     /gene="pcm"
                     /locus_tag="ACHKBL_000190"
     CDS             complement(54064..54690)
                     /gene="pcm"
                     /locus_tag="ACHKBL_000190"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HFO5817118.1"
                     /translation="MVSRRVQALLDQLRAQGIQDELVLNALAAVPREKFVDEAFEQKA
                     WDNIALPIGQGQTISQPYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCS
                     VERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMT
                     QLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA"
     gene            complement(54684..55445)
                     /gene="surE"
                     /locus_tag="ACHKBL_000191"
     CDS             complement(54684..55445)
                     /gene="surE"
                     /locus_tag="ACHKBL_000191"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HFO5817119.1"
                     /translation="MRILLSNDDGVHAPGIQTLAKALREFADVQVVAPDRNRSGASNS
                     LTLESSLRTFTFENGDIAVQMGTPTDCVYLGVNALMRPRPDIVVSGINAGPNLGDDVI
                     YSGTVAAAMEGRHLGFPALAVSLDGHKHYDTAAAVTCSILRALCKEPLRTGRILNINV
                     PDLPLDQIKGIRVTRCGTRHPADQVIPQQDPRGNTLYWIGPPGGKCDAGPGTDFAAVD
                     EGYVSITPLHVDLTAHSAQDVVSDWLNSVGVGTQW"
     gene            complement(55426..56475)
                     /gene="truD"
                     /locus_tag="ACHKBL_000192"
     CDS             complement(55426..56475)
                     /gene="truD"
                     /locus_tag="ACHKBL_000192"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HFO5817120.1"
                     /translation="MIEFDNLTYLHGKPQGTGLLKANPEDFVVVEDLGFEPDGEGEHI
                     LVRILKNGCNTRFVADALAKFLKIHAREVSFAGQKDKHAVTEQWLCARVPGKEMPDLS
                     AFQLEGCQVLEYARHKRKLRLGALKGNAFTLVLREVSNRDDVEQRLIDICVKGVPNYF
                     GAQRFGIGGSNLQGALRWAQTNTPVRDRNKRSFWLSAARSALFNQIVAERLKKADVNQ
                     VVDGDALQLAGRGSWFVATTEELAELQRRVNDKELMITAALPGSGEWGTQREALAFEQ
                     AAVAEETELQTLLVREKVEAARRAMLLYPQKLSWNWWDDVTVEIRFWLPAGSFATSVV
                     RELINTTGDYAHIAE"
     gene            complement(56472..56951)
                     /gene="ispF"
                     /locus_tag="ACHKBL_000193"
     CDS             complement(56472..56951)
                     /gene="ispF"
                     /locus_tag="ACHKBL_000193"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HFO5817121.1"
                     /translation="MRIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHAL
                     TDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYALGNVDVTIIAQA
                     PKMLPHIPQMRVFIAEDLGCHMDDVNVKATTTEKLGFTGRGEGIACEAVALLIKATK"
     gene            complement(56951..57661)
                     /gene="ispD"
                     /locus_tag="ACHKBL_000194"
     CDS             complement(56951..57661)
                     /gene="ispD"
                     /locus_tag="ACHKBL_000194"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HFO5817122.1"
                     /translation="MATTHLDVCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVYA
                     LLAHPRVKRVVIAISPGDSRFAQLPLANHPRITVVDGGEERADSVLAGLKAAGDAQWV
                     LVHDAARPCLHQDDLARLLALSETSRTGGILAAPVRDTMKRAEPGKNAIAHTVDRNGL
                     WHALTPQFFPRELLHDCLTRALNEGATITDEASALEYCGFHPQLVEGRADNIKVTRPE
                     DLALAEFYLTRTIHQENT"
     gene            complement(57680..57991)
                     /gene="ftsB"
                     /locus_tag="ACHKBL_000195"
     CDS             complement(57680..57991)
                     /gene="ftsB"
                     /locus_tag="ACHKBL_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HFO5817123.1"
                     /translation="MGKLTLLLLAILVWLQYSLWFGKNGIHDYTRVNNDVAAQQATNA
                     KLKARNDQLFAEIDDLNGGQEALEERARNELSMTRPGETFYRLVPDASKRAQSAGQNN
                     R"
     gene            complement(58184..58507)
                     /gene="ygbE"
                     /locus_tag="ACHKBL_000196"
     CDS             complement(58184..58507)
                     /gene="ygbE"
                     /locus_tag="ACHKBL_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3561 family protein"
                     /protein_id="HFO5817124.1"
                     /translation="MRNSHNITLTNNDSLTEVEDTTWSLPGAVVGFLSWLCALAIPML
                     IYGSNTLLFFIYTWPFFLALMPVAVVVGIALHSLMDGKLRYSIVFTLATVGIMFGALF
                     MWLLG"
     gene            complement(58557..59162)
                     /gene="cysC"
                     /locus_tag="ACHKBL_000197"
     CDS             complement(58557..59162)
                     /gene="cysC"
                     /locus_tag="ACHKBL_000197"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HFO5817125.1"
                     /translation="MALHDENVVWHSHPVTPQQREQHHGHRGVVLWFTGLSGSGKSTV
                     AGALEEALHKLGVSTYLLDGDNVRHGLCSDLGFSDADRKENIRRVGEVANLMVEAGLV
                     VLTAFISPHRAERQMVRERVGEGRFIEVFVDTPLAICEARDPKGLYKKARAGELRNFT
                     GIDSVYEAPESAEIHLNGEQLVTNLVQQLLDLLRQNDIIRS"
     gene            complement(59162..60589)
                     /gene="cysN"
                     /locus_tag="ACHKBL_000198"
     CDS             complement(59162..60589)
                     /gene="cysN"
                     /locus_tag="ACHKBL_000198"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HFO5817126.1"
                     /translation="MNTALAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLI
                     GRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYF
                     STEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
                     KHLVVAINKMDLVDYSEETFTRIREDYLTFAGQLPGNLDIRFVPLSALEGDNVASQSE
                     SMPWYSGPTLLEVLETVEIQRVVDAQPMRFPVQYVNRPNLDFRGYAGTLASGRVEVGQ
                     RVKVLPSGVESNVARIVTFDGDREEAFAGEAITLVLTDEIDISRGDLLLAADEALPAV
                     QSASVDVVWMAEQPLSPGQSYDIKIAGKKTRARVDGIRYQVDINNLTQREVENLPLNG
                     IGLVDLTFDEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPVSQATATPSEFSA
                     FELELNALVRRHFPHWGARDLLGDK"
     gene            complement(60591..61499)
                     /gene="cysD"
                     /locus_tag="ACHKBL_000199"
     CDS             complement(60591..61499)
                     /gene="cysD"
                     /locus_tag="ACHKBL_000199"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HFO5817127.1"
                     /translation="MDQKRLTHLRQLEAESIHIIREVAAEFSNPVMLYSIGKDSSVML
                     HLARKAFYPGTLPFPLLHVDTGWKFREMYEFRDRTAKAYGCELLVHKNPEGVAMGINP
                     FVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
                     KNQRPELWHNYNGQINKGESIRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLE
                     RDGMLMMIDDNRIDLQPGEVIKKRMVRFRTLGCWPLTGAVESNAQTLPEIIEEMLVST
                     TSERQGRVIDRDQAGSMELKKRQGYF"
     gene            61751..62788
                     /gene="iap"
                     /locus_tag="ACHKBL_000200"
     CDS             61751..62788
                     /gene="iap"
                     /locus_tag="ACHKBL_000200"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase isozyme conversion
                     aminopeptidase"
                     /protein_id="HFO5817128.1"
                     /translation="MFSALRHRTAALALGVCFILPVHASSPKPGDFANTQARHIATFF
                     PGRMTGTPAEMLSADYIRQQFQQMGYRSDIRTFNSRYIYTARDNRKSWHNVTGSTVIA
                     AHEGKAPQQIIIMAHLDTYAPLSDADADANLGGLTLQGIDDNAAGLGVMLELAERLKN
                     TPTEYGIRFVATSGEEEGKLGAENLLKRMSDTEKKNTLLVINLDNLIVGDKLYFNSGV
                     KTPEAVRKLTRDRALAIARSHGIAATTNPGLNKNYPKGTGCCNDAEIFDKAGIAVLSV
                     EATNWNLGNKDGYQQRAKTAAFPAGNSWHDVRLDNQQHIDKALPGRIERRCRDVMRIM
                     LPLVKELAKAS"
     repeat_region   62870..63262
                     /inference="COORDINATES: alignment:crt:1.2"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=62931..62957
                     /rpt_unit_seq="cggtttatccccgctggcgcggggaac"
BASE COUNT        14880 a        17287 c        16471 g        14661 t
ORIGIN      
        1 gggaaactta ctttactgca gattttttga taccgctact aaagccgtat caagtaagag
       61 atggtgcatc cgggaggatt actcgccttg cggctcgccc ttcgggccgt tgctaaagca
      121 acgttatcct ccctggtgcc tgcggttatt accgccacct cttccatacc ttcatcgctg
      181 aaggtacgta gaatatggtg catccgggag gattcgaacc tccgaccgct cggttcgtag
      241 ccgagtactc tatccagctg agctacggat gcaaatggcg gtgaggcggg gattcgaacc
      301 ccggatgcag cttttgaccg catactccct tagcagggga gcgccttcag cctctcggcc
      361 acctcaccac acgcctctta cgagtgcttc gaagaacttg tttatcgctc atcgtcgctg
      421 cgtggcgcac atattacttt ctgagactta taagtcaaac aatttttccc acacttttat
      481 cgtttgcaca cttcacgctc aattagtctg taaaaacgtc aaaaagagtg ttttatcaac
      541 agaaaaacgg aggtctgtca gatagcagta atgcaaaaaa atggagactt aagttgaatg
      601 aacgggagta aagcgaaaag acaatggagt gaagaagaaa ttttgagggt gcgtctcacc
      661 gataaagatg agacgcggaa agattagtaa ctggactgct gggatttttc agcctggata
      721 cgctggtaga tctcttcacg gtgaacagaa acttccttcg gggcatttac gccaatacgt
      781 acctggttgc cctttacccc taaaactgtc acggtgacct catccccaat catgagggtc
      841 tcaccaactc gacgagtcag aatcagcatt ctttgctcct tgaaagatta aaagagtcgg
      901 gtctctctgt atcccggcat tatccatcat ataacgccaa aaagtaagca atgacaaaca
      961 cattacatct aagcagtcat ggcattacat tctgttaaac ctaagtttag ccgatataca
     1021 caacttcaac ctgactttat cgttgtcgat agcgttaatg cgaatgccgt gaagcgagtc
     1081 cacggcattg cctgacgctt atattattgc aatttcgcgc tgacccagcc tttcacactg
     1141 gctaacgctg caggtaaggc cgcagcatcc gtaccaccgg cttgcgccat gtcaggacgt
     1201 ccaccaccct tgccgcccac ctgctgagcg accataccaa tcagttcccc tgctttcaca
     1261 cgatctgtga cgtccttaga tacgcctgca atcagagaaa ccttaccttc ggctaccgtt
     1321 gccagcacga taattgtcga ccccagctga ttttttaaat cgtcaaccat ggtacgcaac
     1381 attttcggct caacaccgct aagctcgcta accaacagct taacaccatt aacatcaatt
     1441 gccttactgg aaagatttgc gctctcctgt gcggcagctt gttctttaag ctgttgtaac
     1501 tctttttcca gctgacgcgt acgttccagt actgagcgca ctttatcagc cagattattg
     1561 ctatcgcctt tcagcagatg cgcgacttcg tttaagcgat cgctgtctgc atgaacggtg
     1621 gcgatagcgc cttctccggt taccgcttcg atacgacgaa cgcctgcagc agtacccgat
     1681 tcagagatga tgcggaacag accaatatca ccagtgcgac tggcgtgagt accgccacac
     1741 agctcggtgg agaaatcgcc catgctcagc acacgtacac gttcatcata cttctcgccg
     1801 aacagcgcca tcgcaccttt cgctttcgcc gcttcgagat ccatgatgtt ggtttcaatc
     1861 ggcaagttac ggcgaatctg tgcgttcacc aggtcttcga ccgcacgaat ctcttctggt
     1921 ttcatcgctt cgttgtgtga gaagtcgaag cgcagcactt tgtcgttaac cagtgaacct
     1981 ttctgcgata catgagtacc cagaacctgg cgcagcgcag cgtgcatcag gtgcgttgcg
     2041 gagtgattca gacgaatacg ggcgcgacga gcttcatcaa catcagcctg caccgcgtcg
     2101 cccactttca gagaacccgc agcaagttta ccgatgtgac caatcgcctg gccgtatttc
     2161 tgcgtatctt ccaccgcaaa ggagaagtta gcgcctttca gttcgccttt atcaccaacc
     2221 tgaccgccgg attccgcata gaatggcgtt tgatccagca cgaccacagc ttcctggcct
     2281 gcattgatgg catcaaccgc tttaccatca acaaacagcg cggtcacttt gccgttcagt
     2341 tccagatggt catagccttt aaattcagat gcactgttaa cacggatcat tgcgttgtaa
     2401 tcggcaccaa agccgctggc ttcgcgcgca cgacgacgct gctcttccat tgctgcttca
     2461 aatccagctt cgtcaacttt gatgttgcgc tcacgacaaa catcagccgt caggtcaacc
     2521 gggaagccat aggtgtcgta cagacggaaa gccgtttcac catccagcgt atcaccagaa
     2581 agttttgcca gctcttcgtc cagcagcgcc agaccgcgct ccagagtacg ggcaaactgc
     2641 tcttcttcag tcttcagcac ctgctcaacc tgcgcctgct ggcgtttcag gtcttcaccc
     2701 gcagagccca ttacgtcgat cagcggacca accagtttgt agaagaaggt ttctttcgcg
     2761 cccagcatat tgccgtgacg cactgcgcga cgaatgatac gacgcagtac ataaccacgg
     2821 ttttcgttgg acggcattac gccatccgcg atcaggaacg cacaagaacg aatgtggtca
     2881 gcgattacgc gcagcgattt gttgctcaaa tcggtcgcgc cagtgacttt cgctaccgcc
     2941 tggatcagcg tacggaacag gtcgatgtca tagttagagt taacgtgttg cagcaccgca
     3001 gcaatacgct ccagacccat accggtatct acagacggct tcggcagcgg ttccatcgtg
     3061 ccatctgcct ggcggttgaa ctgcatgaag acgatgttcc agatctcaat gtagcggtcg
     3121 ccgtcttctt ccggacttcc cggagggcca ccccaaatgt ggtcgccgtg atcgtagaag
     3181 atttcggtgc acgggccgca cggaccagtg tcacccatct gccagaagtt gtcagatgcg
     3241 tatggcgcac ctttgttatc gccgatgcga ataatacgtt cgcgcgggat cccgacttct
     3301 ttttcccaga tttcgtaggc ttcgtcgtcg ctttcataga cggtaaccca cagacgctct
     3361 ttcggcaggg caaaccattt ttcgctggtc agcagttccc atgcaaactg aatggcatcg
     3421 tgtttgaaat agtcgccgaa gctgaagttg cccagcattt cgaagaaggt atggtgacgc
     3481 gcggtgtaac cgacgttttc caggtcgttg tgtttaccac ccgcacgcac gcagcgttgg
     3541 gaagtggtag cgcgggaata attacgcttg tcgagcccaa ggaacacatc cttgaactgg
     3601 ttcatcccgg cgttggtaaa caacaaagtt gggtcgttat ggggtaccag ggagctgctg
     3661 gcaactacct gatgtccctt actatggaaa aagtcgagaa acgcctgacg gatctcagcg
     3721 gtgctcttgc tcataattat cctgaaatca agctaacgaa atatcgccac cagctacagc
     3781 gtgttttaac cgccgggctg gtaactgaaa agtgggaata agataagttt tcttgactgg
     3841 gaagtaaaat cccgtatgcg ttcagtcggc aaaatttcgc caaatatcct ggatatcttc
     3901 catcagatag ccacgataga gcagaaaacg ctggatctta actttttctg aaaagacagt
     3961 tggcaaaggt tcgccatatt ttcgcgtcgc ctgatcgcgc gccagtgcgc accagtcgat
     4021 gtcacattca cgcatcgctt tttctgtcgc ttcgcgggaa atacctttct gattcagttc
     4081 ctgacgaata cgcgcaggtc cataaccttt gcggctacgg ctggcgataa agcgcgcaac
     4141 aaatcggctg tcatcgagat agccatgttc atggcaccag gcgataacgc gctcgtaatc
     4201 ttctgccgtg gcatcaatct cttccgggcc atttttgccc atgatcggtg ccgcgagttt
     4261 acgtcgcaat tcttgctcac tgtgatcgcg caccgccaga atgcgtaccg cacgatccaa
     4321 caggcgagca tatgccgggc gacgggacgt tgattctgtc atggcatatc cttacaactt
     4381 aaaaaagcaa aagggccgca gatgcgaccc ttgtgtatca aacaagacga ttaaaaatct
     4441 tcgttagttt ctgctacgcc ttcgctgtca tctacagaga aatccggcgt tgagttcggg
     4501 ttgctcagca gcaactcacg tactttcttc tcgatctctt tcgcggtttc cgggttatct
     4561 ttcagccagg cagttgcatt cgctttacct tgaccgatct tctcaccttt gtagctgtac
     4621 cacgcgcctg ctttctcgat cagcttctct ttcacgccca ggtcaaccag ttcgccgtag
     4681 aagttgatac cttcgccgta gaggatctgg aattcagcct gtttaaacgg cgcagcgatt
     4741 ttgttcttca ccactttcac acgggtttcg ctccccacca cgttttcgcc ctctttcacc
     4801 gcgccgatac gacggatgtc gagacgaaca gaggcgtaga atttcagcgc gttaccaccg
     4861 gtagtggttt ccgggttacc gaacattaca ccaattttca tacggatctg gttgatgaag
     4921 atcagcagcg tgttggactg cttcaggtta cccgccagct tacgcatcgc ctggctcatc
     4981 atacgtgccg caaggcccat gtgagagtcg ccgatttcgc cttcgatttc cgctttcggc
     5041 gtcagtgccg ccacggagtc aacgacgata acgtctactg cgccagaacg cgccagggcg
     5101 tcacagattt ccagtgcctg ctcgccggta tccggctggg agcacagcag gttgtcgata
     5161 tcaacgccca gtttacgtgc gtagattggg tccagcgcgt gttcagcatc gataaacgca
     5221 caggttttac cttcgcgctg cgctgcggcg atcacctgca acgtcagcgt ggttttaccg
     5281 gaagattctg gtccgtagat ttcgacgata cggcccatcg gcagaccacc tgccccaagc
     5341 gcgatatcca gtgaaagcga accggtagag atggtttcca catccatgga acggtcttca
     5401 cccaggcgca tgatggagcc tttaccaaat tgtttctcaa tctggcccag tgctgccgcc
     5461 aacgctttct gtttgttttc gtcgatagcc atttttactc ctgtcatgcc gggtaatacc
     5521 ggatagtcaa tatgttctgt tgaagcaatt atactgtatg ctcatacagt atcaagtgtt
     5581 ttgtagaaat tgttgccaca aggtctgcaa tgcatacgca gtagcctgac gacgcaccgc
     5641 atcacggtca ccgctgaaac attcccgccg ggtaatgcct tcaccacggg cagtggcaaa
     5701 agcaaaccag acggtgccga caggtttctc ttcactgccg ccatccggcc cggcgatacc
     5761 actaatagac acggcgtaat cagcacgagc cgctttcagt gcgcctatca ccatttccac
     5821 cacgacaggt tcgctcaccg cgccatgctg tgccagcgtc tcttcgcgta caccgatcat
     5881 ttgcgctttt gcttcgttac tgtaggtgac aaatccgcgt tcaaaccagg cggaactgcc
     5941 ggcaatatcg gtaatcactt tcgctaccca accgccggta caagactcag cagttgttac
     6001 ggttgcgcca cgggctttca gcgactgccc aacctgttcg cttaactgca tcagttcact
     6061 gtcagtcatc acaacctctg tcagtcaaaa atttatgccg gacaagataa cactttcgtc
     6121 aggaagatgg tgttggcgtt gcaattttac gaggaggatt cagaaaaaag ctgattagcc
     6181 agagggaagc tcacgccccc ctcttacaaa tagttactgt acgcgcgcca gcgccacggc
     6241 ttgtcctaac tgccagaccg ccattgcgta atgggtgctg tggttataac gggtaatggt
     6301 gtagaagttc ggcagaccgt accagtactg gtagccggtg ccaacatcca gacgcagcag
     6361 gctggcttgt tgatggttgc ccagcggctg ctgtggcgtt aaacctgccg cggcaagctg
     6421 cgaaatgctg tacttggttt tgaagccatt tggcaagcct ggagcctgac cgtttgccat
     6481 caccgcaacc tgatcacctt tcacccagcc gtgcgctttg aaatagttcg ccacactacc
     6541 aatggcatca accggatccc acaggttgat atgcccgtcg ccgctgaaat ctaccgcata
     6601 ttgtttgtaa gacgacggca taaactgtcc gtagcccatc gccccggcaa aggaaccttt
     6661 cagattgagc ggatcgtcct gctcgtcgcg cgccatcagc aggaaggttt ccagctcgcc
     6721 agaaaaatac tccgcgcggc gtgggtagtt aaatgacagc gttgccagcg catcgaggat
     6781 gcgagttttc cccatcacgc gcccccagcg ggtttcaacg ccgataatcc cgacgataat
     6841 ttccggcggt acaccataca cctgccacgc gcgattcaac gcatcttcat actgattcca
     6901 gaaaaccaca ccgttctgca cgttgtccgg cgtaataaat tttttgcgat aacgcagcca
     6961 tgcgccgttc ggacctgatg gcggtttcac cgatgtggtt ggtgcctggt tatccatcag
     7021 ccgcagtacc gaatccagac gtttcgcctg ggagagaatt tcctgcaact gctgacgatc
     7081 gaaaccgtgt ttgttcacca ttttgtcgat gaactgctgg gcattcggat tattggcgaa
     7141 gtcaccgccc atctgcatca cattgtgctg cggctcaagc aggaagccgc cagacggcgt
     7201 tccggtggtc gtttcagtct cagtaggttt tggcttgctg ctacaggcgg caagcaacac
     7261 aaaaagggga agcaatgtta cataacgacg cttgaacatg aggggtccat ttaacagatt
     7321 caaccagggg caagtatggt aaagcatcac gccccgcaca aggaagcggt agtcactgcc
     7381 cgatacggac tttacataac tcaactcatt ctcctcgcta tccttttatt caaactttca
     7441 aattaaaata tttatctttc attttgcgat caaaataaca cttttaaatc tttcaatctg
     7501 attagattag gttgccgttt ggtaataaaa caataaatcc tgaaggagag aacaatgata
     7561 gaaaccatta ctcatggtgc agagtggttt atcgggctgt tccaaaaggg cggagaggtg
     7621 tttaccggga tggtgaccgg cattcttccg ctgttgatta gtctgctggt tatcatgaac
     7681 gcactgatta attttatcgg tcagcatcgt attgaacgtt ttgctcaacg ttgcgccggt
     7741 aaccctgttt cccgttacct gctgttaccg tgcattggca cgtttgtctt ttgcaatccg
     7801 atgaccttaa gcctgggtcg ctttatgccg gaaaagtaca aacccagcta ctacgcggcc
     7861 gcctcttata gctgccactc aatgaatggc ctcttccccc atatcaaccc aggcgaactg
     7921 tttgtttatc ttggcattgc cagcggtctg acaacactga acctgccact tggcccactg
     7981 gcggtgagtt atctgctggt tggtctggtc accaacttct tccgcggctg ggtgaccgat
     8041 ctgaccaccg ccatttttga gaaaaagatg ggcattcaac ttgaacaaaa agttcatctg
     8101 gcaggagcaa catcatgacg cgtattcgga tcgaaaaagg aacgggtggc tggggcggcc
     8161 cgcttgagct ggaagccacg ccgggcaaaa aaatcgtcta tatcaccgcc ggtacccggc
     8221 ctgcgattgt tgacaaactg gcacagctta ctggctggca ggctattgac ggatttaaag
     8281 aaggtgaacc cgcggaggcg gaaattggtg tcgcggtaat cgactgtggc ggcacattac
     8341 gctgcggcat ttatccgaaa cggcgtattc ccaccattaa tatccactcg acgggcaagt
     8401 ccggtccact agcgcaatac attgtagaag atatttatgt ctctggcgta aaagaagaaa
     8461 acatcactgt agtgggtgat gcgacaccac aaccctcttc cgtgggccgt gactatgaca
     8521 ccagcaagaa aatcaccgaa caaagcgatg gtttactggc gaaggtggga atgggtatgg
     8581 gttctgccgt tgccgtgttg tttcaatctg gtcgtgacac catcgacact gtattaaaaa
     8641 ccattctgcc gtttatggca ttcgtttcgg cgcttatcgg catcattatg gcttccggtc
     8701 ttggcgactg gattgcccac ggtcttgctc cgctggcgag ccatccactg ggtctggtca
     8761 tgctggcgct catctgctcc ttcccgctgc tttcaccttt cctcggccca ggcgcagtta
     8821 tcgcacaggt tatcggcgta ttgattggcg tgcagattgg tctcggcaat attccgccgc
     8881 atctggcttt acctgcactg tttgccatca acgcgcaggc ggcctgcgac ttcatcccgg
     8941 tcggtttgtc gctggcggaa gcccgtcagg acacggttcg cgtcggtgtc ccttctgttc
     9001 tggtgagccg ctttttaacc ggtgcgccaa ctgtactgat cgcctggttt gtctccggtt
     9061 ttatctatca atagaggctg aaacatgacc gttatttatc agaccaccat cacccgtatt
     9121 ggcgcgagtg ccactgacgc cctcagcgac cagatgctca tcacctttcg tgaaggcgcg
     9181 ccagcggatc tcgaagagta ttgcttcatt cattgccacg gcgagttgaa aggtgcactc
     9241 catcccggtt tgcaattttc actcgggcag catcgctatc cggtgaccgc cgttggcagc
     9301 gtggcggaag acaaccttcg cgaactgggt catgtcaccc tgcgcttcga tggtttaaac
     9361 gaagcggaat ttccgggcac tgtccatgtg gcaggccctg tacccgacga tatcgcgccg
     9421 ggatcggttt tgaagtttga atctgttaag gagtaaaaaa tgaatcaggt tgccgttgtc
     9481 atcggtggtg ggcaaacctt aggcgcgttc ctgtgccacg gtctggctgc cgaggggtat
     9541 cgcgtcgcgg ttgtcgatat tcagagcgac aaagccgcaa atgtggcaca agaaattaac
     9601 gccgaatatg gtgaaggtat ggcgtacggt tttggtgctg acgccactag cgagcaaagc
     9661 gttctggcgc tctctcgtgg ggtagatgaa atctttggtc gcgtggattt gctggtctac
     9721 agcgccggaa tagcaaaagc agcctttatc agcgacttcc agctcggcga ttttgaccgt
     9781 tcgctacagg tgaatctggt gggttatttc ctgtgtgccc gtgaattttc gcgtttgatg
     9841 atccgcgacg ggattcaggg gcgcattatt cagatcaact cgaaatccgg caaagtgggc
     9901 agcaaacaca actctggcta cagcgcagcg aaatttggtg gcgtcgggct gactcaatca
     9961 ctggcgctgg atctggcgga gtacggcatt acggtgcatt cactgatgct cggtaatctg
    10021 ctgaaatcgc cgatgtttca gtcgctattg ccacaatatg cgaccaagct gggtatcaaa
    10081 ccggatcaag tcgagcagta ttacatcgac aaagtaccgc tcaaacgcgg ctgcgattat
    10141 caggatgtgc tgaatatgct gctgttctac gccagtccta aggcgtcgta ctgcactggt
    10201 cagtcgatca atgtcaccgg cggtcaggtg atgttctgat taacagcgga gatccgttaa
    10261 ggatctccgc gagactaata aaatgcctga tgcgctacgc tcatcaggca tacaggactt
    10321 ccgccactac attaaggaaa agttatggta tccgcactca tcaccgtcgc cgttatcgcc
    10381 tggtgtgcgc aactggcctt aggcggttgg caaatttctc gttttaaccg tgccttcgac
    10441 acactatgcc agcaagggcg ggttggcgtg ggccgttcca gcgggcgctt taaaccgcgg
    10501 gtcgtggtcg ccatcgccct ggacgatcag caacgcatcg tcgacacctt gtttatgaaa
    10561 ggactgaccg tcttcgcccg accacaaaaa attcccgcaa ttaccggtat gtatgcgggt
    10621 gatttacagc ccgatgtgat ctttccccat gatccactat cacagaatgc tctatcattg
    10681 gcgcttaaac tgaaacgtgg ataatttcgt tgtgaatgtt acttgcttgc gaagttatca
    10741 ttttgaaacc taaatcaggt aatcacgccc atgaaacctc gtcagcgtca ggccgccatt
    10801 ctggagtatc tgcaaaagca gggtaaatgc tcggttgaag aattggcgca atactttgac
    10861 accacaggca caaccattcg caaagatctg gtcattctgg aacatgccgg aaccgtcatt
    10921 cgtacttatg gcggagtggt gttgaataaa gaggaatccg atccgccaat cgatcataaa
    10981 acactcatca acacccacaa gaaagagctg attgcagaag ctgccgttag ttttatccat
    11041 gatggcgatt cgatcattct tgatgctggc agtaccgttt tgcagatggt ccccctgctc
    11101 tcgcgcttta ataacatcac agtgatgacc aacagcctgc atatcgtcaa tgcgctatcc
    11161 gaactggata acgaacaaac tatcctgatg ccaggcggaa catttcgtaa aaaatcggcc
    11221 tcatttcacg ggcaactggc agagaatgcc ttcgagcatt tcaccttcga taaattgttt
    11281 atgggcaccg acggcatcga tctcaatgcg ggcgtaacca cgttcaacga ggtttatacc
    11341 gtcagtaagg caatgtgcaa tgccgcgcgc gaagtgattt tgatggcgga ctcatcaaag
    11401 tttggccgta aaagccccaa cgtagtttgc agtcttgaaa gcgtcgataa gctaattacc
    11461 gacgcaggta tcgatccagc gtttcgtcag gcgctggaaa agaaagggat cgacgtgatc
    11521 ataaccggag agagcaatga gtgaagcact actgaacgcg ggacgtcaga cgttaatgct
    11581 ggagttgcag gaagcaagcc gtttaccgga acgtctgggc gatgattttg ttcgcgccgc
    11641 caatatcatc ctgcactgcg aaggcaaagt ggtggtttcg ggaattggca aatcgggcca
    11701 cattggtaag aaaatcgccg caacgcttgc cagcaccggc actcctgcct tttttgtcca
    11761 tccggcagag gcattgcacg gcgacctggg gatgatcgaa agccgcgatg tgatgctgtt
    11821 tatctcttac tccggtggcg cgaaggaact ggatctgatt attccgcgtc tggaagataa
    11881 atctatcgcg ctgctggcga tgaccggcaa accgacgtca ccgctgggcc tggcggcgaa
    11941 agcggtgctg gatatctccg tagaacgcga agcctgcccg atgcaccttg cgccgacctc
    12001 cagcaccgtc aataccctga tgatgggtga cgcgctggcg atggcggtca tgcaggcgcg
    12061 cggatttaat gaagaagatt ttgcccgctc ccaccctgct ggagcactgg gcgctcgctt
    12121 gctgaataaa gtgcatcatc tgatgcgccg tgacgatgcc atcccgcagg tggcgttaac
    12181 cgccagcgtg atggacgcga tgctggaact tagccgcacc ggtttaggtc tggtggcagt
    12241 gtgcgatgat caacgactgg tgaaaggcgt ctttaccgac ggtgatttac gtcgctggct
    12301 ggttggcggc ggcgcactca ccacgccagt caatgaagcg atgacggtcg gcggcaccac
    12361 gttgcaatcg caaagccgtg ccatcgacgc caaagaaatc ctgatgaaac gcaaaatcac
    12421 cgccgcaccg gtagtggatg aaaacggcaa actcaccggc gcaattaacc tgcaggattt
    12481 ctatcaggcc gggattattt aatccttcag ccccagacgt ttcgccagcc gatgcaggtt
    12541 ggcgacgtcg gtttccagca tccgcgcaca ggcggcccag ttgtgatgat tttgcgccag
    12601 tgcctgacga atggtttcac gctggaacgc ttctgtcgct tcacgcaggt tttgcttaac
    12661 gataggcacc gccgccgctt ctggcggcgg caacgtcacc tcaggaaaag caaaatgttg
    12721 cgcctcaaga atcacttcat cgccgctgcg agtggctctc gacagtacta ccgcccgatg
    12781 aatagcatgt tccagttcgc gcacgtttcc cggaaaactg tagtgttgca gtaaatttcg
    12841 cgccccggca cttaatacca cgcgggagag ccccagccgc aaacgacact gctcgcagaa
    12901 ataccccgcc agcagaatga catcatcgcc ccgctcacgc agcggcggca ccgaaagtgg
    12961 aaacacgctc aggcgatgaa acaaatcggc gcggaatcgc cctgccagca cctcttcgcg
    13021 taaatcgcgg ttagtcgccg ccagcacgcg cacatcgacc cgcaaactgc ggtcatcgcc
    13081 aacgcgctga atatcgccat actgcaacac cctcagcagc ttggcctgca atgccaacga
    13141 caactcgccg atctcatcga gaaacagcgt gccgttatcc gccatttcaa acttcccgct
    13201 gcggttactg atagcaccag taaacgcccc tttcacatgc ccgaacaact cactttccgc
    13261 cacactttcc ggcagtgcgg cacagttgag atagaccagc ggattcaccg cccgtggcga
    13321 ggcttcatga atcgctttcg ccaccagctc cttaccggtt cccgtctcac cgctaattaa
    13381 aacgttgaga tcggatgccg ccacaatctc aatctctttt ttcaattgcg tcatgcctgg
    13441 ggacaaacca atcatctgcg tctgtttcac cgcttcaaac ggcgcggcat cgcctggcag
    13501 catattctgg ctttccagtt gttcaatcag taacgcatta cttaacgctc ccgccgccag
    13561 tgcggcaatc agccgtagct cttcgtcgct gaaaacatcg aactgatcgg gctgcatccc
    13621 gtcgagcgtc aaggcaccga tcaggttttg cccggcaaac aacggcaaac caacgcaggc
    13681 gtgaaccttc agactctcct gccctggaat caaaccgtca taaggatcgg gcaattcgct
    13741 gtctgcggga aagcgcacca catcacccgc gcgggcaatc gcttccagcc gtggatgacc
    13801 ttccagcgca aagcgtctac cgagtacatc cttcgccagc ccgtcgatgg caagcggaat
    13861 aaactgccgc gagtcgtaac gtagcaacgc agacgcatcg cactccagca cctgacgcag
    13921 cgtggtgatc aggcgctgaa aacgatcctg atgaccaatc ccacgctgca attcgatggc
    13981 gatattcgcc agcacatcaa cggaaaaact catctttgcc tcactgtcaa tttgactata
    14041 gatattgtca tatcgaccat tttattgata gtcattttga ctactcatta atgagcacaa
    14101 ttttatttat agagtaaaaa caattagata aaaaactggc acgcaatctg caatcagcaa
    14161 gacatctttt tagaacacgc tgaataaatt gaggttgcta tgtctattgt ggtgaaaaat
    14221 aacattcatt gggttggtca acgtgactgg gaagtgcgtg attttcacgg tacagaatat
    14281 aaaacgctgc gcggcagcag ctacaacagc tacctcatcc gcgaagaaaa aaacgtgctg
    14341 atcgacaccg tcgaccataa attcagccgc gaatttgtgc agaacctgcg taatgaaatc
    14401 gatctgacgg atatcgatta catcgtaatt aaccatgctg aagaggacca cgccggggcg
    14461 ctgaccgaac tgatggcaca aattcccgat acgccgatct actgtactgc caacgctatc
    14521 gactcgataa atggtcatca ccatcatccg gagtggaatt ttaatgtggt gaaaacaggc
    14581 gacacgctgg atatcggcaa cggcaaacag ctcatttttg tcgaaacgcc aatgttgcac
    14641 tggccggaca gcatgatgac ttacctgaca ggcgacgcgg tactgttcag taacgatgct
    14701 ttcggtcagc actactgcga cgagcatctg ttcaacgatg aagtggatca gacggagctt
    14761 ttcgagcagt gccagcgtta ttacgccaat atcctgacgc cgttcagccg cctggtaacg
    14821 ccgaaaatta ccgagatcct gggctttaac ttaccagtcg atatgatagc gacctctcac
    14881 ggcgtggtat ggcgcgataa cccgacgcaa attgtcgagc tgtacctgaa atgggcggcg
    14941 gattatcagg aagacagaat caccattttc tacgacacca tgtcgaataa tacccgcatg
    15001 atggccgatg ctatagccca gggtattgcg gaaaccgacc cacgcgtagc ggtgaaaatt
    15061 ttcaacgtcg cccgaagcga taaaaacgaa atcctgacca atgtcttccg ctcaaaaggc
    15121 gtgctggtcg gcacttcgac gatgaataac gtgatgatgc caaaaatcgc cgggctggtg
    15181 gaggagatga ctggtttacg ctttcgtaac aaacgcgcca gtgctttcgg ctctcacggc
    15241 tggagcggcg gtgcggtgga tcgtctttcc acacgcctgc aggatgcggg tttcgaaatg
    15301 tcgcttagcc taaaagccaa atggcgaccg gaccaggacg ctctggagct atgtcgtgaa
    15361 cacggtcgcg aaatcgcccg tcagtgggcg ctcgcgccgc tgccgcagag cacggtgaat
    15421 acggtggtta aagaagaaac ctctgccacc acgacggctg acctcggccc acggatgcag
    15481 tgcagcgtct gccagtggat ttacgatccg gcaaaaggcg agccaatgca ggacgttgcg
    15541 ccaggaacgc cgtggagtga agttccggat aacttcctgt gcccggaatg ctcccttggc
    15601 aaagacgtct ttgacgaact ggcatcggag gcaaaatgag caacggcatt gtgatcattg
    15661 gttcgggctt cgccgcccgc caactggtga aaaatattcg caaacaggac gcctgtattc
    15721 cattaaccct gattgccgcc gacagcatgg atgagtacaa caaaccggac ctcagccatg
    15781 ttatcagcca gaggcaacgt gccgatgacc ttacccgcca gacggcgggt gaatttgccg
    15841 agcagtttaa tctgcacctg tttccgcaca cctgggtaac ggatatcgat gccgaagccc
    15901 atgtggtgaa aagtcagaat aatcagtggc aatacgacaa gttagtgctg gcaaccggtg
    15961 ccagcgcctt tgtcccgcca gtgcccgggc gtgagttaat gctgacgtta aatagtcagc
    16021 aagagtatcg cgcctgtgaa acgcaactgc ggaatgcccg acgcgtgttg attgttggcg
    16081 gtggcttgat tggtagcgaa ctggcgatgg atttttgtcg tgcaggcaaa gcggtcacgc
    16141 taatcgacaa cgctgccagt attctggcag cgttaatgcc accggaagta agcagccgct
    16201 tgcagcatcg gttgacggag atgggcgttc atctgctgtt aaaatctcag ttgcaggggc
    16261 tggaaaaaac ggattctggc attctggcaa cgctggaccg ccagcgcagc atcgaagtgg
    16321 atgcggtaat tgccgccacc ggactgcgcc cggaaaccgc cctggcacga cgcgccgggc
    16381 tgacgattaa tcgcggcgtt tgcgtcgata gttatctgca aaccagtaat gccgatattt
    16441 atgcgctggg cgattgcgcg gaaattaacg gtcaggtatt gccgttcctc cagccgattc
    16501 aacttagcgc gatggtgctg gcaaaaaatc ttctcggcaa taacacgccg ctgaaactcc
    16561 cggcgatgct ggtgaaaatc aaaacgccgg aattaccgct gcatctggca ggcgaaaccc
    16621 agcgtcagga tttacgctgg caaattaata ccgaacgcca gggaatggtg gcgcgcggtg
    16681 ttgatgatgc cgaccagctt cgcgcctttg tggtcagtga ggatcggatg aaagaggcgt
    16741 ttggattgtt gaaaacgttg ccggtgtagg cgagctacag tgccccaaat gtcggatgcg
    16801 gcgctgacgc gtcttatccg acctacaggg aacgcatgtg ttgatcacat aatacgttta
    16861 tgtcgcatcc tgcactgtcc caaaatgtcg gatgcgacgc tggcgcgtct tatccgacct
    16921 acggggacgc acgtgtaggc cggataaggc gtttacgccg catccggcaa tggtgtccaa
    16981 atgcaacacg ctttatccgt tctggacttc acccgctaac cagcgcgccg cagcaataac
    17041 cccctgcccc agagacaatc caccatcgcc cgccggtaaa ctctgcggga agagcaacgt
    17101 aaaatcagca agataatgcg ccagacgtgc acgcagcaaa cggttatgaa taactccgcc
    17161 gctaaatacc agcgtagtga taccccgcat cgtggcctgc tcacgcatca acgcggcaaa
    17221 accctgcgcc agcgcatcat gaaacgccca cgcgcgttga ttaaccggag cctgccagct
    17281 cagccactgc tgccagaaag tggcgagatc cagttgattg tccaccagcg gcatcgtcac
    17341 cggatgcgtc actccgtcgc acgaagctgc gagcgcctcc agagcacaag ccgcttcacc
    17401 ttcataactt aacgtggctg gcgcacagcc cagtcccgcc gccacagcat cgaacaaacg
    17461 cccacacgat gacgccagcg gcgcgttaat tccacgctca atggcccgcg ccagcacgct
    17521 ccagttttgc tgttgcacac ttgccgtttc agggtaattc tgccactccg gcacaaagcg
    17581 caggcactgc gccagcaggt tacgccacgg ctgcttcgcc gccaaatcgc ccccagcaag
    17641 cgccactgca ggcaagccgc ccaggtgctg gcattcgcga tagttcaccc gcaggcactc
    17701 gccgccccac aaagcgccgt tctcccccat accgataccg tcgagcgtca acgcaatgac
    17761 atcaccgcca tccagtggcc agtgatgttc tgccagacac gccgccgcat gagcatgatg
    17821 atgcagcacc gtttgcgtcg gcagattcat ctcacgcgcc cactggctgg agacatagcc
    17881 cggatgcgcg tcatgcacaa cgtattgcgg ggtgaaatcg tagatgtttt gcatcaggcg
    17941 taacgcttcg cgccactgca tctggatgcc atcgtcactt aaatcgccca gatgctggct
    18001 caacaccgct tgttcaccgc gcaccaggca gaaggtattt ttcagatccg cgccgagaca
    18061 cagcacaggc ggaacatttt taaagcccgg aggcaaagcc agcgcatccg gcacataccc
    18121 ccgcgaacgg cgcagcattt cgccgctttc gcgcaccacc gaatcatcca tccgctgcac
    18181 gatgtcgcgg ttatgtatca agaatccgtc ggcaatgccc tgcaaatccg ccagcgcctg
    18241 ttcgttgctg atagccggtg gtttaccgct caggttgccg gaggtcatca ccagcgggca
    18301 ttgcagttcc tgtaacagca aatgctggag cgggttcgca ggcaacatca ccccaacttc
    18361 gttaaggcca ggggcgatat catcacaaag ctcaggaacg tattttttat ccaccagcac
    18421 aatcggcgcg gcgggtgtgg taagcaactg acgggcagcg tctggtaaac cttctgccac
    18481 tggcaacata accgccagcg gtttcgccgg gcgatgtttg cgcgcccgca gtgtcgccac
    18541 cgcgttactg ttacgcgcat cgcaggcgag atgaaatccg ccaatccctt tgatggcgac
    18601 aatgttgccc atttttaact gtgcgatagc tgcctgtaat gccgcctctt gttctgcatg
    18661 ttcaccatga cttacccatt caagatgggg gccacactcc gggcaggcca ccggctgggc
    18721 gtggaagcga cgatcgagcg ggtcacggta ctctttgtca caggccggac acagcggaaa
    18781 cgccgccatc acggtaaacg ggcggtcgta aggcatggcg cgaataatgg tgaaacgcgg
    18841 accacagtga gtacagttga taaacggata acgccagcgt cgttcgcctg gggtattcat
    18901 ttcggcaaga caagcagggc aagtcgcggc atccgggaca atttgcgtat tcatgacgcc
    18961 acccgcgctc tggcggatgg tgaactcagt gggcagttgt gaccagataa acggctcacg
    19021 ctcgacgcta tcaatacgcg ccagtggcgg gcagtgctga tgcaactgca caagaaacgt
    19081 ttccggatct tccagcagcc ggacttctac gccatcgccg tcattacaga catcgccgtg
    19141 aagatttaat tgctgtgcca gttgccagac aaacggacga aaaccgacgc cctgcacttt
    19201 gccacgaata cgcagttgga caccgcaaga tgtgtttttt gccattgagt tattcccgcc
    19261 atcatgaatt gcgtaacccg cccagccgga cacgacagcg tcgcatccgg cagtcacagg
    19321 tcggcgatac tgcccgcttc gtattctacg aatatttccg ggaattcctt tgatgccaga
    19381 acagttcagt aagattttta gaacatcagc gccgtacggc gacgtttttc tgcgctcagt
    19441 tgttcaagtt tattacgatc gacacaaatc agcgcatggg tcgggcaagc cgccatacac
    19501 gccgggccgt cttcacgatg gttgcacagg tcgcatttat tggcttcggc tttgtcagcc
    19561 cgtacattca gccccgcgcc gctgttgcga atcaccggac gtaccaccac ttccatcgcg
    19621 ccatacgggc aagccacaac gcaggttttg caaccaatac aacgttcctg catcacatga
    19681 acaaaccctt tatcacgact gatagcacca ttcggacaga cgttagcgca cggtgcatct
    19741 tcacactgac ggcaaactgt cgccgtggaa atgttcacac ctttaatgac atggattcgc
    19801 ggtaaaaaag tttccggggt cagcgatgca cagtcctgat tttcctgatg agaaaccacg
    19861 cacgccactt cacaggtacg gcaaccaata catttactcg cgtcagcaat gatgaaacgg
    19921 ttcatcaaat tctccagcaa tgacagttaa tgcgccgata cattcacaaa tcatgccagt
    19981 ttttaattta ctgttattta aagaaattaa attctgtaat gcaggaaaaa cgatgtcatc
    20041 gacactagtg acgatgacat gtgatgacaa tgtttatcgc gaaagagcaa tgagggagtc
    20101 gcggcggatc agttttccgc tgaaggtttt cggcggtgtg taagcaccac catcgagcat
    20161 aaatatcagc cgcccaatta tttcctgaat catctcagtc accggaattt ttacgctgga
    20221 gagcgccgga acggtgtagg gggcaatagc gatatcatcg aatccgataa ctgacacctg
    20281 ctctggcacc gctacgccgc gctcgtgtaa cgctttcatc gcacctatcg ccatatcgtc
    20341 gttactggca actaacgcgc taaatttagc cccacgttcg agcaacattt ctaccccttc
    20401 ggccccgctg gcaggcgtcc atttaccgtt agcgataagt ttttcattga gcgcaatacc
    20461 atgctgcgcc agcgcgtctt tatacccggc aagacgttca acgctggtgg gggaatccat
    20521 cgaaccggta aggaaagcaa tctcctgatg tccggcgttt atcaactctg ccacggcgtt
    20581 aaagctggtc tgtttatgat cgcaccagac gctatggctg ctgtttttgc gcaggcggcg
    20641 attaagcacc attatcggct gactgtgtgc gtcaatgatg tcatcaatct catccacgct
    20701 taaaaaacgc gggtaaatca taatggcgtc gcagcgcaaa tccagcagat actgaatcgc
    20761 ctggcgctct tcttcggcgc tgtgtttacc atctgccaat agcaactgtc gccctttctc
    20821 ttccgccatt cgcgcggcat gaaagagtaa ttcactaaaa taaatgccgt ggtaaagcgt
    20881 gttggtcacg accagcccca gcgtctgagt actcttcgcc gacagattgc gcgccagcaa
    20941 gtttggacgg taaccgctct cttctaccgc ctgaaacacg cgatctttag tctcctggct
    21001 gacgtagcca ttacctgaaa gcacgcggga aacggtcgct tttgaaaccc cggcgcgctt
    21061 cgccacttcc agcatcgtcg tcatcatttt catcccttta cacgcaatca acgcagtgta
    21121 ctgcaccgtt tgccgattgt ccttgcgcaa tcggcgggaa aaatattcag gtgaccggtt
    21181 tcacaaatat aaaaaatgaa caattaactc tcttgcttat tcagtgacaa ctattcatga
    21241 ttttgtgaaa ccggtttcct aattccgttt cagcatcggc atttttccgt cacgtcgact
    21301 gataacaact acatctaccc tactgataac aggataaaat ccgatggcca aaaattatgc
    21361 ggcgctggca cgctcggtga tagcggcact gggcggcgtt gataacatct cggcagtcac
    21421 gcactgtatg acgcgtttgc gctttgttat caaagatgac cagcttatcg acagtccgac
    21481 gttgaaaacc atctccggcg tgctcggcgt ggtacgtagt gacaaccagt gtcaggtgat
    21541 tatcggcaat accgtttcac aagcctttca ggaagtcgtc agcctgctgc cgggagatat
    21601 gcagcccgca cagcccgtgg gtaaacccaa acttacgcta cgtcgcatag gtgcggggat
    21661 cctcgatgcg ctgatcggca ccatgtcacc gctgatcccg gcgattatcg gcggatcgat
    21721 ggtcaaactg ctggcgatga tcctcgagat gagcggcgtg ctgacaaaag gatcgccgac
    21781 cttaaccatt ctgaatgtga ttggtgacgg tgctttcttc ttcctgccgc tgatggtcgc
    21841 ggcttcggcc gccatcaaat ttaaaaccaa tatgtcgctg gcgattgcca tcgcgggtgt
    21901 tctggtacat ccgagtttta ttgaactgat ggcgaaagcg gcccagggtg aacatgtcga
    21961 atttgccctg atcccggtca ccgcggtgaa atacacctac acggtgatcc cggcgctggt
    22021 gatgacctgg tgcctgtcat atatcgaacg ctgggtggat cgcattacac cggcggtgac
    22081 taaaaacttc ctcaagccaa tgctgattgt gttgattgcc gcaccgctgg caatcctgct
    22141 gattggcccg attggtatct ggatcggtag cgccatttcg gcgctggttt acaccattca
    22201 tggttatctg ggctggcttt cagtcgccat tatgggcgcg ctgtggcctc tgctggtaat
    22261 gaccgggatg caccgcgtct ttacgccaac catcattcag accattgccg aaaccggcaa
    22321 agaagggatg gtcatgccgt cagagatcgg cgctaacctg tcgctgggcg gttcatcact
    22381 ggcagtggcg tggaaaacga aaaacccgga actgcgtcag acggcgctgg ctgcggcggc
    22441 atcagccatt atggcgggga tttccgaacc ggcgttatat ggcgtggcga tccgcctgaa
    22501 acgtccgctt atcgccagtc ttatcagcgg ttttatttgc ggcgcggttg ccggtatggc
    22561 ggggcttgcc agccactcaa tggcagcgcc ggggctattt accagcgtgc agttcttcga
    22621 tccggcgaat ccaatgagca tcgtctgggt gttcgccgtc atggcgctgg cggtcgtgct
    22681 gtcgttcatc ctcacactgt tgctcggctt tgaggatatt cctgttgagg aagcgactgc
    22741 cgaggcgcga aagcatcaga gcgcacaacc gaccgtcgcc aaagaagtaa gtcttaattg
    22801 aggatgaaaa tgtcagtatt tccagaaggt tttttatggg gcggcgcgct tgccgccaac
    22861 cagtctgaag gtgcgtaccg ggaaggtggc aaagggctga ccaccgtcga tatgatccca
    22921 cacggcgagc atcgaatggc ggtgaaactg gggctggaaa aacgttttca gttgcgcgat
    22981 gacgagtttt atcccagcca tgaggcgacg gatttttatc atcgttataa agaagatatc
    23041 gccctgatgg cagagatggg attcaaagtg ttccgtacct caattgcctg gagccgcctc
    23101 ttcccgcagg gcgatgaact gacgcccaac cagcagggca ttgcttttta ccgttctgtc
    23161 tttgaagagt gtaaaaagta cggtatcgaa ccgctggtca cgttgtgcca cttcgatgtg
    23221 ccgatgcatc tggtcaccga atatggctcc tggcgtaacc gcaagctggt ggagtttttc
    23281 agccgctacg cccggacctg ctttgaagca tttgatggtc tggtgaaata ctggctcacc
    23341 ttcaatgaaa tcaacattat gttgcatagc ccgttctccg gcgcgggtct ggtgtttgaa
    23401 gaaggcgaaa atcaggatca ggtgaaatat caggctgcgc atcaccaact gattgccagt
    23461 gcattagcca ccaaaatcgc ccatgaggtt aacccgcaaa atcaggtggg ctgtatgctg
    23521 gcgggcggta acttctaccc ttacagttgc aagccggaag atgtctgggc ggcgctggag
    23581 aaagatcggg aaaacctgtt ttttatcgat gtgcaggcgc ggggcgcgta tccggcttac
    23641 tctgcccgcg tattccgcga aaaaggggta accatcgaca aagcaccggg cgatgatgag
    23701 atcctgaaaa acaccgtcga ttttgtctct ttcagctatt acgcctcacg ctgcgcctcg
    23761 gcggagatga acgccaacaa cagcagtgcg gcgaacgtgg tgaaatcgct gcgtaaccca
    23821 tatctacagg taagcgactg gggctggggg attgatccac tcggtctgcg tatcaccatg
    23881 aacatgatgt atgaccgcta tcagaagccg ctgtttctgg tggaaaacgg cctcggcgca
    23941 aaagatgaac ttgctgccaa tggcgagatt aacgatgact atcgcatcag ctatttacgc
    24001 gaacatatcc gcgcaatggg cgaagcgatt gcagatggca ttccgctgat gggctacacc
    24061 acttggggct gtattgattt ggtttccgcc tcaacgggtg aaatgagcaa acgctacggt
    24121 tttgtctacg tagaccgtga cgacgcaggc aacggtacgc tggcgcgcac gcgtaagaaa
    24181 tcgttctggt ggtataaaaa agtgattgcc agtaatggtg aggatttaga gtaagtaaca
    24241 gtgccggatg cggcgtgaac gccttatccg gcctacatgt cgcacgaacc aggtaggccc
    24301 gataagcgca gcgcatcggg caatctggcg tttgctatca gttctgattc cctcatcaag
    24361 aacatccctg tcctgattcc ttactgaaaa actactcttc ttccaccgct aactgcgcga
    24421 atccgccatt cccttcccag ccttccagtc gttgataaac ggtttctacc gcatctttaa
    24481 tcggctgggt catcgggtag taaaagccga cgatatccgg ctggatgccg aggaaaatca
    24541 cttcgccaat gtcttctttt aactggtcga taaggtagtt caacggcatg ttatgggtag
    24601 tcatcataaa catctcggcg atatcatccg gatcgatgat gcggatctcg ccggggttta
    24661 accccatatc cgtggcgtcg acaatcagca gccgtgtcgg gcgcagttcg cggatggcga
    24721 cgatgtcgtt ttccggagcg ctaccgccgt caatcaccac ccagttacct ttcggcgcgg
    24781 cggcgcactt ttccgccagc agcgggcctg cgccatcatc gcccatcatg ctattgccaa
    24841 cacaaagtaa aacgtcagtc acgcagtctc ctcaccatca aatagatggc gttttcctga
    24901 tgaatatcat gcagcatact taacagcatt gtactccatt ctttctgctg cgaagtttgc
    24961 gctgcccggg ctttatcgaa tgcgttagcc agcatcggca catggttaat gtcgatgacg
    25021 atctcaccat atttcggcac gccttccatt ttgcggcggg cttcgctgcc cgcctccaga
    25081 gtggcaatcc atgccagata gtcatcccac gggcaggtaa gcgccgcttc caggcaatcg
    25141 ataaccccaa ggtggtgacc aatcgccagg ctgtaataga ccacctgctg cgcctcggcg
    25201 ggcgtagcat cgttctcatc aataaattta cggctcagtt gactgaacac caccttttca
    25261 ctcatcggat acgcgcctct tcaacaacat gattcagatg gctgacaatc tcgttcagac
    25321 gcggatcgtt ttccgcttcc agccagcgtg caacctgttc ttcgccctgc cctaactgtg
    25381 taaggtaatc atcggcaatc tgacgaccgt aacgataacc cgccaggcgg cgtgcttcgc
    25441 gatccacttt cacacgcagc ggctgcacca tatcgccatg caggatctcc gccggttgtt
    25501 catcctgctc acccggccca cgggcgtgaa ttttctgctc cagcaggccg agcgccattg
    25561 caaagccgta tagcgtagcg gcaggcgttg gcgggcagcc agggatgtag acatctaccg
    25621 ggacgatttt atccgtaccg ccccacacgc agtagagatc gtggaagatc ccgccactgt
    25681 taccgcaggc accgtaggag atacagattt tcgggtccgg cgcggactgc cacgcacgca
    25741 gcgcagggga tcgcattgca cgggtgaccg cgccggtaaa cagtaaaata tccgcatgac
    25801 gcggtgaagg aacgacttta atgccgaagc gttctgcatc aaacagcggc gaaagcgtgg
    25861 cgaaaatttc gatttcgcag ccgttgcagc caccgcagtc cacgcggtaa acgtaggcag
    25921 aacgtttgat ttttttcagt aacgacgcct tcatgctggc gatggattca tccaccgtca
    25981 tcgggaccgg aatgccgttg gcgtcacggg ggcctaataa attgctcatc agatggcctc
    26041 tttcatatgg cgagtcagtt caatacggtc ggacggcacc aggcatttct ggcgcttaca
    26101 ttccgggcaa gtctcaaagc tttcgcggtg gttttccgcg cggctgtcac cattgtgctt
    26161 aagcagcgca atggcgtagt cgatctcttt ctggacggcg aaaggacgat tgcagacgcg
    26221 gcagttgcac agcgcgaagc gggactgttg gaggaagtct tctttcttcc acaccgccag
    26281 ttcgtactct tgcgacagtt tgatcgccgc cgtcgggcaa acttcttcgc agcgtccaca
    26341 gaagatgcag cgcccgagat taaactccca ggccagctct cctgtggcga ggtcggtttc
    26401 aaccgttaag gcgtttgacg ggcaggcatt gacgcaggcc gcgcagccga tgcactgctg
    26461 cgggttctgc tctggcttac cacggaagtt tttatcaacc gcaatcggct ccagcggata
    26521 agacgaggtc gccgtgccgg ttttgatgac ttttttgata aaggtaaaca tggcgattcc
    26581 ttatttcagc ggcgagtttt tacgctcaat gctgtaacgc tcgagttctt tgtacggcac
    26641 cactttgctc ttcttcttac gcacatcgac cacggtcatg cggtcggtac aggagtagca
    26701 agggtccagg ctaccgataa tcagcggcgc atcggaaacg gtgttgccgc gcagcatgta
    26761 gcgcagggtc ggccagttcg cgtaggtcgc ggcacggcag cgccagcggt acagcttctg
    26821 gttgtcgccg gtcatgctcc agtggatatc gtcgccgcgc ggcgcttcgg caaagcccag
    26881 cgcaaaacgg tgcggaatgt aggtaaaacc ttctaccatc aacggaccgc ccggcaggtt
    26941 atccagaccg tagtcgatca tgttcagcgc ggtatagacc tcgttgattc gcactttcag
    27001 acgggaaata acgtcgcagc cctgctcgct gtggacttcc attggcagca ggccataacc
    27061 gacaaacggg tgatcggcgc gagtatcgcg ggcgtgaccg ctggcgcgaa ccatcgggcc
    27121 aacgttactg aaatcgcggg cgatttccgg gtccagacga ccaatgccga cggtgcgctg
    27181 ttccatgttc ggtgtgctca gcagcacatc caccagctcc tgcacttcac gacgcatctg
    27241 ttgtgccagc tggcgggtct ggatcatgtc gtctttcagc agatcgcgac gaatcccgcc
    27301 gatcaggttc aggccgtagg ttttacgtgc accggtaagg atctctgcca ttttcatgga
    27361 ggtttcacgc acgcggaaga actgcataaa gccggagtcg aagccggtaa agtgacaggc
    27421 gaggccgaga ttgagcagat gcgagtgcag gcgttccacc tccaacagaa tggcgcggat
    27481 catctgcgca cgttctggca cctgaatacc catcgcgttt tccaccgacg tggtgtaggc
    27541 ggtgctgtga gcaaagccgc agatcccgca cacacggtca gagaggaagg tcacttcgtt
    27601 gtaacccata cgggtttccg ccagtttttc catgccgcga tggacgtaga acaggcggta
    27661 gtcggcgtcg ataatgtttt caccatcgac aaacagacgg aagtgacccg gttcgtcaga
    27721 agtgacgtgc aggggaccaa tcggcacgac gttgtttttc ttgtcgccca gttcgttgat
    27781 gaactcgtag gtttcagcat cggtagtcgg tgccggacgc tgacgataat ccatgctgtc
    27841 tttacgcaga ggataaagtt catccggcca gtcatccggc agcaccagac ggcgttcatc
    27901 cggcagacca accggaatca aaccgtacat atcgcgcact tcacgctcgc cccacactgc
    27961 cgccggaacg cgcggcgtca cggaaggata ctccggtttg ttggcgtcaa cttcgacgcg
    28021 caccgttacc caacacttag tccccttctc catcgacagc acgtaataaa cggcgtaatg
    28081 accattcagt ttgcgttcgt cgttaccaaa cagcaccgac agccagcccc cctgcttgta
    28141 gtagagaaac tccaccactt ccggcaggta gttcaccttc acggtcacgg tcagctgatc
    28201 tttggtctgc caggcgtggt ccagcacgac gcccggaaat gcctcattca gcgcggcgag
    28261 ataatgttga cctaattttt cttcagacat gctcaaactc tctttaatca cgccgccagc
    28321 aaggagacga acgctaaaaa tgcaaagcca aaaccagccc aggtaatgcg tggagtaata
    28381 tcaagacgca aacgggccat gctgttttcg aacagcgcga taaccagtac gccgaccacc
    28441 agtttgacga tggcaatcac cagcgccagc agcagcccac cgacggtgaa ggtttccatt
    28501 tgtccccacg gaataaacac cccgacgaac atctgcaaca ccaccagctg tttcaggctg
    28561 atgccccatt tcatgacgcc aaagccgctg ccgctatact cagagagcgg accttcctgc
    28621 aactcctgct cggcttccgc caggtcgaac ggcagtttgc ccatttcgat aaaagtggcg
    28681 aacgcacagg cacaaagcgc cagcaccagc gggatgctct ggctcagtgg ccagtgataa
    28741 acggtgtcgg tgatgttgct gatgttggtg gaaccggcaa cctgtgcggc gacccacaga
    28801 ccaagcagca gcatcggttc aaccaacacg ccaagcatcg cttcacggct cgcgccgata
    28861 gcggtaaacg ggctaccagt atccagacca gaaatggcaa agaagaaacg cgcgatggcg
    28921 aagagataca gtaaggtgat caaatcgccc agttgcggca gcggagagcc gacggtcacc
    28981 accggcagcg cagtggcgat agtcagcatg acgcccacca tcacatacgg cgtcaggcgg
    29041 aacacccagc cggaggcatc cgggccaacg ctctgacgcc ccagcagttt gataatgtcg
    29101 cgatactcct gcaacacgcc cggcccacga cggttatgca agcgggcgcg cgctacgcgg
    29161 gtaataccgg agagcagcgg cgcaacggca aataacacca gcgcctgaat taacggatat
    29221 aaaacactca ttctcaggct cctcgtgaaa caataatcac caccagtacc gccagttcaa
    29281 ccagcgccat ccggcggaac agcaacgcac tcccctcgca ctgccagccc ggcaccagcg
    29341 acaccggatt cagccatttg cgtagtttca gcaccggcgc aaacgcctgt ttcaccggca
    29401 tggcaaaacc gtgagcggta atcaccattg atttttcatg atcgtagccg cacacccagg
    29461 ccgcgccgcg ggaacgcgac ggcaaacgat cgcctttgca aatcgccata atgatgaatg
    29521 gcagcagcgg gcaggcaatc agcagcaacg tgatcatggg ttgagaaacg gtggtgttag
    29581 caggctccag cggcagaggt acagcagcag agagcatcgg cagtagccac ggcgcagcaa
    29641 caccgccaat tacgcagcaa atcgccagtg ccactacgct tacgctcatc aggagcggcg
    29701 cacaggtggc gttttcggcc tctttggtac gcggcgcgcc gaggaaagta acgccataaa
    29761 ctttcgccat acacatcacc gccagcgcac cggtaattgc cagccccacg gcgagcagcg
    29821 gcccgagaag acgggcaaca aacgcgccac tattgctcag tttgaagaag gattgataga
    29881 taacccattc cccggcaaac ccgttcagcg gcggcagcgc agccattgcc atcagcccga
    29941 ctaacatggc gatggagata accggcattt tcttgccaat accaccgagt ttttcgatat
    30001 cgcgatgacc ggtacggaac cagacgctcc ccgcaccaag gaacagtacg ctcttgaaca
    30061 ggctatggtt aagcagatgg tacagaccac caaccagacc aagggcaatc agcgccggtt
    30121 gttcgagcgc gatacccgtt acgccagcgc ccagccccag caggatgatg ccgatatttt
    30181 ccagggtgtg gtaagccagc agacgttgga tattatgttc catcaacgca tacagaccgc
    30241 cgacgaatgc ggtgatcatg ccgagtacca gcagtgcgat cccccaccac agcggtgcat
    30301 taccgcccag tagtgacaga gttaaaatgc ccagcaggcc aattttcatg actaccgtgg
    30361 aaaacagcgc ggcagctggc gcagaggcgt tggcatgtgc ctgtggcacc cagccgtgca
    30421 gcggaataat cccggccagc aggccaaagc caatcacacc gagcagccag atatcggaac
    30481 cgagcggcag ctgttgcata cgcatatcca gcaggcgcag atccagcgtg ccgtaacgct
    30541 gccacagcag ccagcaggca atcgccagca gcagagtgcc aagacgcccc agcgcaaacc
    30601 acagtttgcc ctctttgctg ttgctggtga ggaacaccgc acacagggcc atgatttcgg
    30661 ccattaccac gaacatgccg aggttgctgg cgatgacggc gcagacggcg gcagccatca
    30721 acatattgat ctgcaagccg ttgcacttca cctgcgcgtg gcgatgccag tcaatgttgt
    30781 agaggctgac aaacagaccg cataggccga gcgtaatcag ccaaatcgca ttaagcggag
    30841 agatttgcac atcgtagctt accagcgaca gcgcaccgct cacgccaacc gcgccagtca
    30901 gcactgtgaa gcccgcggct gccgtataca ggctaccaac cgcgccgcca attccggcta
    30961 tccagccgct tagcgccttt tgaaaagaaa agagaaatgc cagaacagcg gcggcgacaa
    31021 accacgccac gccgctattg atcagggaaa ttgcgctcat ttagcctctc cactttgagc
    31081 ctgctgaaac aaggtgagat cgccaaaatc cgtgttaaaa gtcagctcac gcttacgttt
    31141 gctgacgcgg gcgatatcgg tgttatccac cagatgcagg gctttagtcg ggcacatccg
    31201 cacgcaggcc ggaccttgtt catcaaagct acaaaggtca catttgacgg cgatcgcgcg
    31261 aatacctggc acccagtcaa gcaatgtgct gacacgcgcc ggagcaggcg gtgccggtgg
    31321 cgctttcggg gtattggcgt ttgccggaat atccagcgga cggctgccgg aaaattcaat
    31381 tgcgccaaac gggcaggcga tgccgcacag tttgcagctt acgcacagac tttcattcaa
    31441 ctgcacggcc ccatcaacgc gggtgatggc gttaaccggg caaaccaccg cacagggcgc
    31501 atcttcacag tggtgacaga gctgcggcgc agattctttt tcattgagca tcactcgcag
    31561 gcgcggcatt gattgcaggc cgtgctggcg atgcgtctct gaacaggcgg cctcacaggt
    31621 gtggcagccg atacagagcg tggagtcagc aattacaaaa cgattcacca ggcattcctc
    31681 aggtgattgt catttttgac gaaaacatgc cgatgaaatg tcattttcga cactcatcga
    31741 cacgcccatc cccaaacagg cgtaacgcct gcaaaacggg caaagcctca gctcatgctg
    31801 ccgggctttg tccctttacc agttggctta aattcaccgg cacattgttt tcaatggcgg
    31861 tgtataagct gtcgtaaacg ccagcgattt tttcgagata gtgttccaga acctgttcgc
    31921 gatcgcggat gttgaccttg ccatcaatca tgccgtcaat gctcagttgc gcctgcgcaa
    31981 tccgctgttt gtcttcacca tcttttgttt cgacgtaata ctcgatggtg tggctgttaa
    32041 agccatccgc cagggtgacg taaatacgaa aatgttcaaa aaggacgaaa cagagttctt
    32101 tgtccggcgc gcagctcatg gcgtgatgca ggcgcgcttt cgataacgtg attccctgaa
    32161 ccagcgaatt gcagtagatg tgccactggt cctgtaggcg acgatgccgc tgtgcgatgt
    32221 aatcggcttt ctcgcttatt tcccaaatag tcattgtcag gttacccgtt taacagagat
    32281 gccagcttta agcatttttt gtgccaactt ttaatttatt gttattaaag cgatttttaa
    32341 gctaaagatg aatttcgtcg ccgtgtcgac gtgtcatttc gacatcatcg acattattca
    32401 ccgcagggat aatcaacact ggcacaatta ttgcttgtag ctggcaatag ttaatgggag
    32461 gcgatatgca cgaaataacc ctctgccaac gggcactgga attgatcgaa cagcaggccg
    32521 caaaacacgg cgcaaaacgc gtaactgggg tctggctcaa aattggcgca ttttcttgtg
    32581 tcgaaaccag ctctcttgcc ttttgttttg atctggtttg ccgcggcagc gtggcggaag
    32641 gttgtaaact gcacctcgaa gaacaagagg ccgaatgctg gtgtgaaaca tgccaacagt
    32701 atgtgacgct actgacccag cgcgtccgcc gctgtccaca gtgtcatggt gacatgctac
    32761 agattgtggc agacgacggt ttacagattc ggcggataga aatagaccag gagtgagcga
    32821 tgtgtacaac atgcggttgc ggtgaaggca acctctatat tgagggtgat gaacataacc
    32881 ctcattccgc gtttcgtagc gcgccatttg ccccggcggc acgcccgaag atgaaaatca
    32941 ccggcattaa agcgcctgaa tttaccccca gccagactga agaaggcgac ctgcattacg
    33001 gtcatggcga agcgggcact cacgcgccgg gcatgagcca gcgtcggatg ctggaagtcg
    33061 aaattgacgt gctggacaaa aataaccgtc tggctgaacg caaccgcgcg cgctttgctg
    33121 cccgcaagca actggtgctc aacctggttt ccagccctgg ttccggtaaa accaccctgc
    33181 tgacggaaac tttaatgcgc ctgaaagaca gcgttccgtg cgcggttatt gaaggtgacc
    33241 agcaaaccgt gaacgatgcc gcacgcattc gcgctaccgg caccccagcg attcaggtga
    33301 acaccggtaa aggctgccat cttgacgcac agatgattgc cgacgccgcg ccgcgcctgc
    33361 cactggacga taacggtatt ctgtttatcg aaaacgtcgg taacctcgtt tgcccggcca
    33421 gcttcgatct cggtgaaaaa cacaaagtgg cggtgctttc cgttaccgaa ggtgaagaca
    33481 aaccgctgaa atatccgcac atgtttgccg ccgcctcgct gatgctgctc aacaaagttg
    33541 acttgctgcc gtatctcaat ttcgacgttg agaagtgcat cgcctgcgcc cgtgaagtca
    33601 atccagaaat tgaaatcatc cttatttccg ccaccagcgg cgaagggatg gaccagtggc
    33661 tgaactggct ggagacacag cgatgtgcat aggcgttccc ggccagatcc gcactattga
    33721 cggcaaccag gcgaaagtcg acgtctgcgg cattcagcgc gatgtcgatt taacgttagt
    33781 cggcagctgc gatgaaaacg gtcagccacg cgtgggccag tgggtactgg ttcacgttgg
    33841 ctttgccatg agcgtaatta atgaagccga agcacgcgac actctcgacg ccttacaaaa
    33901 catgtttgac gttgagccag atgtcggcgc gctgttgtat ggcgaggaaa aataatgcgt
    33961 tttgttgatg aatatcgcgc gccggaacag gtgatgcagt taattgagca tctgcgcgaa
    34021 cgtgcttcac atctctctta caccgccgaa cgccctctgc ggattatgga agtgtgtggc
    34081 ggtcataccc acgccatttt taaattcggc ctcgaccagt tgctaccgga aaacgttgag
    34141 tttatccacg gtccgggttg cccggtgtgc gtactgccaa tgggtagaat cgacacctgc
    34201 gtggagattg ccagccatcc ggaagtcatc ttctgtacct ttggcgatgc catgcgcgta
    34261 ccagggaaac aggggtcgct gttgcaggca aaagcacgcg gtgccgatgt gcgcatcgtt
    34321 tactcgccaa tggatgcgtt aaaactggcg caggagaatc caacccgtaa agtggtattc
    34381 ttcggcttag gttttgaaac caccatgccg accaccgcca tcactctgca acaggcgaaa
    34441 gcccgcgatg tgcagaattt ttacttcttc tgccagcaca ttacgcttat cccgacgctg
    34501 cgcagtttgc tggaacagcc agataacggt atcgacgcgt tcctcgcgcc gggccacgtc
    34561 agtatggtta tcggcactga tgcctataat tttatcgcca gcgattttca tcgcccgctg
    34621 gtggtcgctg gtttcgaacc ccttgatcta ctgcaaggcg tggtgatgct ggtggagcag
    34681 aaaatagcgg cccacagcaa ggtagagaat cagtatcgtc gggtggtacc ggatgccggt
    34741 aacctgctgg cgcaacaggc gattgccgat gtgttctgtg tcaacggcga cagtgaatgg
    34801 cgcggcttag gcgtgattga atcttctggc gtgcacctga cgccggatta tcaacgattc
    34861 gatgccgaaa cacatttccg cccggcaccg cagcaggtct gcgatgaccc gcgtgcgcgt
    34921 tgtggcgaag tcttgacggg caaatgtaag ccgcatcaat gcccgctgtt tggtaacacc
    34981 tgtaatcctc aaaccgcgtt tggtgcgctg atggtttcct ccgaaggagc gtgcgccgcg
    35041 tggtatcagt atcgtcagca ggagagtgaa gcgtgaataa tatccaactc gcccacggta
    35101 gcggcggcca ggcgatgcag caattaatca acagcctgtt tatggaagcc tttgccaacc
    35161 cgtggctggc agagcaggaa gatcaggccc gtcttgattt ggcacagctg gtagcggaag
    35221 gcgaccgtct ggcgttctca accgacagtt acgtcattga cccgctgttc tttcctggcg
    35281 gtaatatcgg caaactggcg atttgcggca ccgccaatga cgttgcggtc agtggcgcaa
    35341 ttccgcgcta tctctcctgt ggctttatcc tcgaagaagg attgccgatg gagacactga
    35401 aagccgtagt gaccagcatg gcagaaaccg cccgcgcggc aggcattgcc atcgttactg
    35461 gcgataccaa agtggtacag cgcggcgcgg cagataaact gtttatcaac accgctggca
    35521 tgggcgcaat tccggcgaat attcactggg gcgcacagac gctaaccgca ggcgatgtat
    35581 tgctggttag cggtacactc ggcgaccatg gggcgactat ccttaacctg cgtgagcaac
    35641 tagggctgga tggagaactg gtcagcgact gcgcggtgct gacgccgctt attcagacgc
    35701 tgcgtgacat tcccggcgtg aaagcgctgc gtgatgccac ccgtggtggt gtaaacgcgg
    35761 tggttcatga gttcgcggca gcctgcggtt gtggtattga actttcagaa gcggcactgc
    35821 cggttaaacc tgctgtgcgc ggcgtttgcg aactgctggg actggacgcc cttaactttg
    35881 ccaacgaagg caaactggtg atcgccgttg aacgcaacgc agcagagcaa gtgctggcgg
    35941 cgttacattc ccatccattg ggaaaagatg cggcgctgat tggcgaagta gtagaacgta
    36001 aaggtgttcg tcttgccggt ctgtatggcg tgaaacgaac cctcgatcta ccgcacgccg
    36061 aaccgcttcc gcgtatatgc taataaaatt ctaaatctcc tatagttagt caatgacctt
    36121 ttgcaccgct ttgcggtgct ttcctggaag aacaaaatgt catatacacc gatgagtgat
    36181 ctcggacagc aagggttgtt cgacatcact cggacactat tgcagcagcc cgatctggcc
    36241 tcgctgtgtg aggctctttc gcaactggta aagcgttctg cgctcgccga caacgcggct
    36301 attgtgttgt ggcaagcgca gactcaacgt gcgtcttatt acgcatcgcg tgaaaaagac
    36361 acccccatta aatatgaaga cgaaactgtt ctggcacacg gtccggtacg cagcattttg
    36421 tcgcgccctg atacgctgca ttgcagttac gaagaatttt gtgaaacctg gccgcagctg
    36481 gccacaggtg ggctataccc caaatttggt cactattgcc tgatgccact ggcggcggaa
    36541 gggcatattt ttggtggctg tgaatttatt cgtcatgacg atcgcccctg gagcgaaaaa
    36601 gagttcaatc gtctgcaaac atttacgcag atcgtttctg tcgtcaccga acaaatccag
    36661 agccgcgtcg ttaacaatgt cgactatgag ttgttatgcc gggaacgcga taacttccgc
    36721 atcctggtcg ccatcactaa cgcggtgctt tcccgcctgg atatggacga actggtcagc
    36781 gaagtcgcca aagaaatcca ctactacttc gacattgacg atatcagcat cgtcttacgc
    36841 agccaccgta aaaacaaact caacatctac tccactcact atcttgataa acagcatccc
    36901 gctcacgaac agagcgaagt cgatgaagcc ggaaccctca ccgaacgcgt cttcaaaagt
    36961 aaagagatgc tgttgattaa tctccacgag cgggacgatt tagcccccta tgaacgcatg
    37021 ttgttcaaca cctggggcaa ccagattcaa accttgtgcc tgttaccgct gatgtctggc
    37081 gacaccatgc tgggcgtgct aaaactggcg caatgcgaag agaaagtctt taccactacc
    37141 aatctgagct tactgcgcca gattgccgaa cgtgtggcaa tcgctgtcga taacgccctc
    37201 gcctatcagg aaattcatcg tctgaaagaa cggctggttg atgaaaacct cgccctgacc
    37261 gagcagctca acaatgttga tagtgaattt ggcgagatta ttggccgcag cgaagccatg
    37321 tacagcgtac ttaaacaagt ggaaatggtg gcgcaaagtg acagtaccgt tctgatcctc
    37381 ggtgaaactg gtacaggtaa agagctgatt gcccgcgcga tccataatct cagtgggcgt
    37441 aataatcgcc gtatggtcaa aatgaactgc gcggcgatgc ctgccggatt gctggaaagc
    37501 gatctatttg gtcatgagcg tggggctttt accggtgcca gcgctcagcg cattggtcgt
    37561 tttgaactgg cggataaaag ctcactattc ctcgatgaag tgggcgatat gccgctggag
    37621 ttacagccga agttgctgcg tgtattacag gaacaggagt ttgaacgtct cggcagcaac
    37681 aaaatcattc agacggacgt acgtttaatt gccgcgacta accgcgatct gaaaaaaatg
    37741 gtcgccgacc gagagttccg tagcgatctc tattaccgtc tgaacgtatt cccgatccac
    37801 ctgccgccac tacgcgagcg tccggaagat attccgctgc tggcgaaagc ctttaccttc
    37861 aaaattgccc gtcgtctggg gcgcaatatc gacagcattc ctgccgagac gctgcgcatc
    37921 ctgagcaaca tggaatggcc gggcaacgtg cgtgagctgg aaaacgtcat tgagcgcgcg
    37981 gtattgctaa cacgcggcaa cgtgctgcag ctgtcattgc cagatattgc tttaccagaa
    38041 cctgaaacgc cgcctgccgc aacggttgtc gcccaggagg gcgaagatga atatcagttg
    38101 attgttcgcg tgctgaaaga aacaaacggc gtggttgccg ggcctaaagg cgctgcgcaa
    38161 cgtctggggc tgaaacgcac gactctgctg tcacggatga agcgactggg aattgataaa
    38221 tcggcattga tttaacagat gtggttatac tcgatcaaca ttcaatgaaa ggaggcagta
    38281 tggcagtttc agcaagaaat caattaaccg gaacagttag cacagtagca atgggcgctg
    38341 tgaatgatga agtagaactg acgctggcag gtggtgcaaa gctggtggca attgtgactc
    38401 acagcagcca gcaagcgctg ggtctggcga aagggaaaga agcgattgcc ctgattaaag
    38461 ccccgtgggt cacgctggca acggaagact gtggactgaa attctctgct cgcaaccagt
    38521 ttgctggcag cgtgtcgaca atcaccgaag gcgcagtgaa cgccactgtc catatcaaaa
    38581 cggatgcggg ttttgaaatt gtggcagtgg taacaaatga aagtcaggat gaaatgaaac
    38641 tcaccactgg ctcgcgcgtt attgcattga tcaaagcatc agccatcctg attgccacca
    38701 aagcgtaaac gccttatttg ccggagagta tggctctccg gcagatttat ttctttggac
    38761 gatatttttc tggcaactcc ggcaccggac gcttgtcatc gatgagatga cgcacggtta
    38821 agatcggatg acgccacagc attctcggcc cggcccaacg cataatctgt ttcatctctt
    38881 cacgctttgc aggctggtaa cagtgcaccg gacactgctt acaggctggt ttctcttcac
    38941 cgaacacaca tttatccagc cgcttttgcg cgtaagcaaa caacgcctcg taatgctccg
    39001 gctctgctga cgcctgcggg catttcgctt gataaagatc gatcattttt ttaatcgtca
    39061 gtttttcacg agagatacgc ttgccggaca tgctgcctcc atactcttaa ggtgcattta
    39121 tattacaact taattttaaa ggggactatg gctctgaagg aggaggtttc ttcaaccgac
    39181 gaatttggcg atcttgtttc atcgtcatca cgcaaaagct gcaaagcagc atttttcgcg
    39241 gaaccgacat caagaactca cctttcgcgt cttcccctga aatgattaac tccggtatca
    39301 tgtgcgcctt atgtgattac aacgaaaata aaaaccatca cactacattt aacatcaggg
    39361 agccggactt aaccccatga gaacaataga aaatttcgac gcccatacgc ctatgatgca
    39421 gcagtatctc aagctgaaag cccagcatcc cgagatcctg ctgttttacc ggatgggcga
    39481 cttttatgaa ctgttttatg acgacgcaaa acgggcgtcg caactgctgg atatttcact
    39541 gaccaaacgc ggtgcttcag cgggagagcc aatcccgatg gcggggattc cctaccatgc
    39601 ggtggaaaac tacctcgcca aactggtgaa tcagggcgaa tcggttgcta tttgtgaaca
    39661 gattggcgat ccggcgacca gcaaagggcc ggtcgagcgc aaagttgtcc gtatcgttac
    39721 gccgggcacc atcagcgatg aagcgctgtt gcaggaacgt caggacaacc tgctggcggc
    39781 tatctggcag gacagcaagg ggttcggcta cgccacgctg gatatcagct ccgggcgttt
    39841 tcgcctgagc gaaccggcgg accgcgaaac gatggcggca gaactgcaac gtacgaatcc
    39901 agcggaatta ctgtatgcgg aagatttcgc cgagatgtcg ctgattgaag gtcgccgcgg
    39961 cctgcgtcgt cgcccgctgt gggagtttga aatcgacacc gctcgccagc aattgaacct
    40021 gcaatttggc acccgagatc tggtcggttt tggtgtggag aacgcaccac gcggactttg
    40081 tgctgccggt tgtctgttgc agtatgcgaa agatacccaa cgcacgaccc tgccgcatat
    40141 tcgttctatc actatggaac gtcagcagga cagcatcatt atggatgccg cgacgcgtcg
    40201 taacctggaa attactcaga acctggccgg cggtgcggaa aatacgctgg cttcagtgct
    40261 cgactgtact gtaacgccga tgggtagtcg tatgctgaaa cgctggctgc atatgccagt
    40321 gcgcgatacc cgcgtgttgc ttgagcgcca gcaaactatt ggcgcattgc aggatttcac
    40381 cgccgagctg cagccggtac tgcgtcaggt cggcgacctg gaacgtattc tggcgcgtct
    40441 ggctttacga actgctcgtc cacgcgatct ggcccgtatg cgtcacgctt tccagcaact
    40501 gccggagctg cgcgcgcaac tggaaggtgt cgatagcgcc ccggtacaag cactgcgtga
    40561 gaagatgggc gagtttgccg aactgcgcga cctgctggag cgagcaatca tcgacacgcc
    40621 gccggtgctg gtacgcgacg gtggtgttat tgcgactggt tataacgaag aactggatga
    40681 atggcgcgcg ctggctgacg gcgcgaccga ttatctggag cgtctggaag tccgcgagcg
    40741 tgaacgtact ggcctggaca ccctgaaagt cggctttaac gcggttcatg gttattacat
    40801 tcagatcagc cgtggacaaa gccatctggc acccatcaat tacatgcgtc gccagacgct
    40861 gaaaaacgcc gagcgctaca ttattccaga gctaaaagag tacgaagaca aagttctcac
    40921 ttcaaaaggc aaagcactgg cactggaaaa acagctttat gaagagttgt tcgacctgct
    40981 gttgccgcat ctggaagcgt tgcaacagag cgcgagcgcg ctggcggaac tcgacgtgct
    41041 ggtgaacctg gcggaacggg cctatacgct gaactacacc tgcccgacct ttattgataa
    41101 accgggtatt cgcatcaccg aaggccgcca tccggtggtt gaacaggtgc tgaatgagcc
    41161 gtttatcgcc aacccgctga acctgtcgcc gcagcgtcgc atgttgatca ttaccggtcc
    41221 gaatatgggc ggtaaaagta cctatatgcg ccagaccgca ctgattgcgc tgatggccta
    41281 catcggcagc tatgtaccgg cacaaaaagt cgagattgga cctatcgatc gcatctttac
    41341 ccgcgtaggg gcagcggatg atctggcatc cgggcgttca acctttatgg tggaaatgac
    41401 cgaaaccgcc aatattttac ataacgccac cgagtacagt ctggtgctga tggatgagat
    41461 tgggcgtgga acgtccactt acgatggtct gtcgctggcg tgggcgtgcg cggaaaatct
    41521 ggcgaataag attaaagcgt tgacgttgtt tgctacccac tatttcgagc tgacccagtt
    41581 accggagaaa atggaaggcg tcgccaacgt gcatctcgat gcactggagc acggcgacac
    41641 cattgccttt atgcacagcg tgcaggatgg cgcagcgagc aaaagctacg gcctggcggt
    41701 tgcagctctg gccggcgtgc caaaagaggt tattaagcgc gcacggcaaa aactgcgtga
    41761 gctggaaagc atttcgccga acgccgccgc tacgcaagtg gatggtacgc aaatgtcttt
    41821 gctgtcagtg ccagaagaaa cgtcgcctgc ggtcgaagca ctggaaaatc tcgacccgga
    41881 ttcactgact ccgcgtcagg cgctggagtg gatttatcgc ttgaagagtc tggtgtaata
    41941 acaattcccg atagtctttt gctatcggga atattaacga caactgacga atcaaataaa
    42001 aataccctgt ataataggaa atcttatttt acagggtaaa accatgccat ctacacgcta
    42061 ccaaaaaatc aatgcccatc actatcacca tatatgggcc gttggtgata ttcatggtga
    42121 ctatcaatta ttacaatccc gcttacatca actctctttt tgtcctgaaa ccgacttgct
    42181 tatttctgtc ggcgataaca tcgatcgtgg gcccgagagt cttaacgtgc tgcgcctgtt
    42241 aaaccagccc tggtttatct ccgtcaaagg caaccacgaa gcgatggcgc ttgaggcatt
    42301 cgaaactggc gatggaaata tgtggcttgc cagcggtggt gactggtttt tcgatttaaa
    42361 tgattcagag caacaagagg caatagatct gttgctgaaa ttccatcacc ttccacatat
    42421 tattgaaatc actaacgaca ccataaaata tgtcatcgca catgcagatt atccggggaa
    42481 ggagtatcag tttggcaaag aaatagcgga gagcgaatta ctctggcctg ttgatcgtgt
    42541 gcagaaatca cttaatagcg aattacaaaa aataaacggc gctgattatt ttatatttgg
    42601 acatatgatg tttgataaca ttcagacgtt cgcaaaccag atttatattg ataccggatc
    42661 gccgaaaagc gggcggctgt cattttataa aataaagtag tctcatgctt cttctgtgaa
    42721 gcatgagtaa cccggcgtta ttgcaggtca ttattcattt ttcgctacca gcaaagagag
    42781 atccagcttc gccagcgcgc ggctggcact ctccggcaga ccatcgtcag taatgatctg
    42841 atcaaactcg cttaatggta acgccagcca tgtcgccacc tgtccatatt tcgtcgcatc
    42901 acagaccaaa actcgctggc ggctggcact ggcaatcgcc cgtttcaccg tgactttatc
    42961 ttctgctggc gtagagatcc cccgcacact ccatgacgat gcagaaataa aagcctgatc
    43021 aatcatcagg ctacgcagca tggtcgcagc ggcttcccca acacaggaac ggttttcccg
    43081 acacactgcg ccgccagtgt gaataattgt gcaattactg ttgtcgagca agtagtccgc
    43141 aataacgaag tcgttagtaa ccactgtgag tggctccatg tgagtcagat gctgtgcaat
    43201 cgctaacgtg gtcgttcccg catccagata gatacaacgt cccggctgaa caagacttgc
    43261 cgccagcttg ccaatagccg ctttttgcgt cattgccagc gcagttttta cctgatgaga
    43321 aggttcatgc gccacccgtc ccggagactg gacgcccccg gacaccaaca caacggctcc
    43381 ctgctgctcc agtttttgta aatcccgacg aatggtcata tgtgacacat tcattctgtc
    43441 cgttagttca gcaatactaa caatgccttt ttcagctacc atctcaagga tgatttggcg
    43501 acgctctacg ggtatcaact tttgctcctt cctttgtcct gctgacattc tacgctattt
    43561 gcctgcgaaa cgtgcgcggc gcaactaacg cttagttcac ataaaataac atacaatgtt
    43621 aatttttgtg aatcagctca ccatatcgtt atctttccgc gcttatattc acaacatcaa
    43681 acaaaatatc acttaaatta acaaggagag cagatgaaaa cgggatcgga gtttcatgtc
    43741 ggtatcgttg gcttagggtc aatgggaatg ggagcagcac tgtcatgtgt ccgcgcaggt
    43801 ctttctacct ggggcgcaga cctgaacagc aatgcctgcg ctacgttgaa agaggcaggt
    43861 gcttgcggag tttctgataa cgccgcgacg tttgccgaaa aactggacgc tctgctggtg
    43921 ctggtggtca atgcggccca ggttaaacag gtgctgtttg gtgaaacagg cgttgcacaa
    43981 catctgaaac ccggtacggc agtaatggtt tcttccacta tcgccagtgc tgatgcgcaa
    44041 gaaattgcta ccgctctggc tggattcgat ctggaaatgc tggatgcgcc agtttctggt
    44101 ggtgcagtaa aagccgctaa cggtgaaatg actgtcatgg cctccggtag cgatattgcc
    44161 tttgaacgac tagcacccgt gctggaagcc gttgccggaa aagtttatcg cataggtgca
    44221 gaaccgggac taggttcgac cgtaaaaatt attcaccagt tgttagcggg cgtacatatt
    44281 gctgccggag ccgaagcgat ggcacttgca gcccgtgcgg ggatcccgct ggatgtgatg
    44341 tatgacgtcg tgaccaatgc cgccggaaat tcatggatgt tcgaaaaccg gatgcgtcat
    44401 gtggtggatg gcgattacac cccgcattca gccgtcgata tttttgttaa ggatcttggt
    44461 ctggttgccg ataccgccaa agccctgcac ttcccgctgc cattggcctc aacagcattg
    44521 aatatgttca ccagcgccag taacgcgggt tacgggaaag aagacgatag cgcagttatc
    44581 aagattttct ctggcatcac cctaccggga gcgaaatcat gatcaagatt ggcgttatcg
    44641 ccgatgattt taccggcgcg acggatatcg ccagttttct ggtggaaaac ggtctaccaa
    44701 cggtacaaat taacggtgtt ccaacaggta aaatgccgga agcaatcgac gcactggtga
    44761 tcagcctgaa aacgcgctcc tgtccggtgg ttgaagccac acagcaatcg ctggcggctc
    44821 tgagctggtt gcaacagcaa ggttgcaaac agatctattt caaatactgc tctactttcg
    44881 acagttcggc gaaaggtaat atcggcccgg ttaccgatgc attaatggat gctctcgaca
    44941 cgccgtttac ggtcttctct ccggccctgc cggtcaacgg acgtacggtt tatcaggggt
    45001 atttgttcgt aatgaatcaa ctgctggccg aatccgggat gcgccatcac ccggtaaatc
    45061 ccatgaccga cagctatctt ccccgtctgg ttgaagcgca atccacaggg cgctgcggcg
    45121 tcgtttcggc acatgttttc gaacaaggtg tggatgccgt tcgtcaggag ctggctcgct
    45181 tacagcaaga gggctaccgc tacgcggtgc ttgatgcgct gaccgaacac catctggaaa
    45241 ttcagggaga agccttgcgc gataccccac tggtaacggg cggttctggt ctggcgattg
    45301 gccttgcccg gcagtgggcg caagaaaacg gtaaccaggc tcgcgaagca gggcgtccgc
    45361 tcgctgggcg cggcgtagtg ctctccggtt catgctctca aatgaccaac cgccaggtgg
    45421 cacattaccg tcaaattgca ccagcccgtg aggttgatgt ggcacgctgc ctctcaaccg
    45481 aaactctggc cgcttatgca cacgagctgg cagagtgggt tctgggccag gaaagtgtac
    45541 ttgctccact ggtttttgcc accgccagca ctgacgcatt ggcagcaatt caacagcaat
    45601 acggtgcaca aaaagccagt caggcagtgg aaacactgtt ttctcaacta gcggcgcggt
    45661 tagcagcgga aggcgtgaca cgctttattg tcgcaggcgg tgagacctcc ggcgtagtca
    45721 cacagagcct gggcataaaa ggttttcata ttggcccaac catttccccc ggcgtgccgt
    45781 gggtaaacgc actggataag cctgtctcac tcgcccttaa atctggcaac ttcggtgatg
    45841 aagccttttt ttcacgagcc caaagagagt ttttatcatg agcgatttcg caaaagtaga
    45901 gcagtctttg cgagaggaga tgacgcggat tgccagttca ttctttcagc gcggatatgc
    45961 aactggttcg gctggcaatc tgtcgctgct tttacctgac gggaatttac tggcgacacc
    46021 gacaggttca tgcctgggca atctcgatcc gcagcggctt tccaaagtca ccgcggatgg
    46081 cgaatggtta agtggtgaca aaccctcgaa agaggtgctc tttcatctgg cgctgtatcg
    46141 caacaatccg cgctgtaaag cggtagtgca tttgcacagt acgtggtcga cggcgctttc
    46201 ctgcctggaa gggctggaca gcaataacgt tattcggccg ttcacaccat acgtggtgat
    46261 gcggatggga aacgtcccgc tggtgcccta ttaccgaccg ggcgataaac gcatcgcaca
    46321 ggatctggcg gaactggcag cagacaatca ggctttttta ctggcaaatc atggcccagt
    46381 ggtttgcggt gaaagcctgc aagaagccgc caacaatatg gaagagctag aggaaacggc
    46441 aaagctgatt tttattctcg gtgaccgccc gatccgttat ctgaccgcag gtgaaattgc
    46501 ggaattaagg agttaatgca atgcctcgtt ttgcagctaa tttatccatg atgttcaccg
    46561 aagtgccttt tattgaacgc ttcgccgcag cgcgaaaagc cgggttcgat gctgtggagt
    46621 ttctgtttcc ctatgactac tccccccagc aaatccagca gcaactggcg caaaaccacc
    46681 tgacgctggc gctgttcaat accgctcccg gagatattaa tgcgggagaa tggggattat
    46741 ccgcccttcc cggacgtgag cacgaagcac gtgcagacat cgacctggca ctggaatatg
    46801 cattggcgct caattgtgaa caagtccatg tgatggcagg cgtcgtgccc gctggcgaag
    46861 atgctgaacg gtaccgggca gtatttattg ataatctccg ctacgctgcc gaccactttg
    46921 cgccacacgg caagcgaata ttggttgaag cactcagccc cggcgttaag cctcattatc
    46981 tcttctccag ccagtatcag gcactggcta ttgttgaaga ggttgcgcga gataacgttt
    47041 ttattcaact agatactttc catgcgcaaa aagtagatgg caacctgacg catttaatcc
    47101 gcgactacgc cgggaaatat gcgcacgtac aaattgccgg actacctgat cgacatgaac
    47161 cggacgatgg agaaatcaac tatccgtggc tgttccgcct gttcgatgaa gtgggatatc
    47221 agggctggat cggttgtgaa tacaaacctc gtggcctcac cgaagaagga cttggctggt
    47281 ttgacgcctg gcgctaattc gttaaatccc taattacaac gtacccatac atccccccat
    47341 ctacggatgg ggtaggttaa ctataacttt cagacagggt ttcccatgtc cacaattaca
    47401 ttgttatgca ttgcgttaac tggcgtaatc atgctgttgc tgctggtcat caaggcaaag
    47461 gtacaaccat tcgttgctct gctcctcgtc agcctgttag tcgcacttgc ggcgggtata
    47521 ccggcgggcg aagtgggtaa agtgatgatc gccgggatgg gcggtgtgct tggttccgtc
    47581 actattatta ttggtctggg cgctatgctg gggcgtatga tcgaacactc tggtggtgca
    47641 gagtcactgg ctaattattt cagtcgcaag ttaggcgaca aacgaactat cgctgcgctg
    47701 actctggcag cgttcttcct cggtattccc gtcttctttg atgtcggctt tattattctt
    47761 gcgccaatca tttacggttt tgccaaggtt gccaaaatat caccactcaa atttggtctg
    47821 cctgtcgctg ggatcatgct cactgttcac gtggcggtac cgcctcatcc aggccctgtc
    47881 gccgcagcgg ggttactcca cgcagacatc ggctggctaa ccatcatcgg tattgcgatt
    47941 tctattcccg tagggattgt tggctacttt gcagcgaaaa taatcaataa gcgccaatat
    48001 gcgatgtcgg tcgaggtact ggaacagatg caactggctc cggccagtga ggaaggcgcg
    48061 acaaaattaa gcgataaaat aaatccaccg ggcgtcgcgc tggtcacctc gctaattgtt
    48121 attcctatcg cgattatcat ggcgggtacg gtttccgcaa cactgatgcc gccttcgcat
    48181 cccctgcttg gtacgctaca gctgatcggc tcaccaatgg tagctctaat gattgcgctg
    48241 gtgctggcat tctggttatt ggctttacgt cgcggctgga gcttacaaca caccagcgac
    48301 attatgggct cagcccttcc cactgcggcg gtagtgattt tggttactgg tgctggaggg
    48361 gtatttggca aagtgctggt ggaatcgggc gtcggtaaag cccttgccaa catgttacaa
    48421 atgattgacc tgcctctgtt accagccgca tttattattt cactggcgct gcgtgcatcg
    48481 caggggtcag caaccgtagc aatcctgaca accggcgggt tactctcaga agcggtgatg
    48541 ggattgaatc caattcagtg cgtattggtg acgctggcag cctgctttgg tgggcttggc
    48601 gcgtcacata ttaatgactc agggttctgg attgtgacca aatatctggg gttgtcggta
    48661 gcagacgggc tgaaaacctg gacagtgtta acgaccattc tcggttttac cggattctta
    48721 attacctggt gcatatgggc ggtaatttga ccttaataaa aggtccgatg ggcatcggac
    48781 cttttattta gcgcttacct tccgccagca gcggcgggat gctcggcacc atcggcgcgt
    48841 catcaatatc tttctgcgtc atgcggaacg cttcgggata atgttcgcgg ctggtgcggc
    48901 gtagcggttc ggtatcgcgc caggtataga ggcaatgctg gcactggtag accatccaga
    48961 catcttttac cggcgatttc gccatcactt caatctgttc atcggcacaa cgtggacaaa
    49021 tcatcttgtt ctccttattg acgtgcggcc agcatagcgg tcagtttttc agcccaggct
    49081 ttggtttccg gtaagtccac caccggctgg ctgtagtgac cacggttgtc cggggcgaca
    49141 ggcgtggtgg cgtcgataat cagcttgtcg gtgatccccg ccggacttga gccagggtcg
    49201 agttccagca cggacatatt cggcaactgt accaaatccc ctgccgggtt cactttcgag
    49261 gagagcgccc acatcacctg cggcaggttg aacggatcaa cgtcttcatc gaccataatc
    49321 accatcttca cgtagcccag accgtgcggc gtggtcatcg cacgcaggcc caccgcgcgg
    49381 gcaaagccgc cgtagcgttt tttggtggag ataatcgcca gcaggccgtg ggtgtacatg
    49441 gcgtttaccg cctgcacttc cgggaactcg gctttcagtt gttgatacag cggcacgcag
    49501 gtggcaggcc ccatcaggta gtcgatttcg gtccacggca taccaagata gagcgattcg
    49561 aaaatcggtt tggtacggta agagacttta tcgatacgca ccacggtcat gttacgcccg
    49621 ccggagtagt ggccggtaaa ctcaccgaac ggcccttcaa tctcacgttt acgactttcg
    49681 ataacccctt cgaggatcac ttccgagccc cacggcacat caaaaccggt cagcggggcg
    49741 gtggcgatcg ggtacgggct ttcgcgcagc gcgcctgcca tttcgtactc agactgatcg
    49801 tatttcagcg gcgtggcccc cattagggtg atgatcggat cgttaccgag tgtgatggca
    49861 atcggcagat cttcaccgcg ctcttctgct ttatgcagat gcagggcgat atcgtgcatc
    49921 ggcaccggtt gcaggccgag cttacgcttg cccttcactt ccatgcggta gatgccgacg
    49981 ttctgcttgc cgaagttatc cgggtcgagc ggatcgcggg aaaccacgca cgctttatcg
    50041 agatagaaac cgccatcacc gtcgtttaaa cgaaacagcg gcagaatatt gaacaggttg
    50101 atctcgtcgc catcgacggt gttctgcgcc caggctggat tggcgcggcg ctccggggca
    50161 atggggaagt tatcccagcg gcggataaac tcatcaatct gttttttaac cggggtgttt
    50221 ggcggcaggc cgagggaaat cgcgtggttc tgccaggaac cgatggtgtt cattgccacg
    50281 cgggcatcga taaagccgcg aatattatca aaccaaagcg cgggcgcgcc gtcgccgata
    50341 cgcccggtgg cgttggctgc ggctgccaga tccggttccg cgttcacctc ttcgctgatt
    50401 ttcagtaact ggccgtggtc atcaagcgcc tgtaaaaagc tgcgtaaatc atcaaatgcc
    50461 attattcatt ctcctgagaa aaattccggg cctgcggcaa tccttgccag cgcctggcgt
    50521 gaggatgttc gaggccaaat tgatccagca cgcgggctac cacgtggtgg acaatgtcat
    50581 ctaccgtttc gggatggtta taaaaggcag gcatcggcgg caccatcgcc acgcccatgc
    50641 gcgaaagtgc gagcatattt tcgagatgga ttgtgctaag cggcatttca cgcggcacca
    50701 gcaccagttt gcggccttct ttgagcacga cgtccgccgc gcgccccacc aggccatcgg
    50761 cgtaaccagc gcggataccg gcgagcgttt tcatactgca tggaataacg atcatgccgt
    50821 cggtacgaaa ggagccggaa gagatagtcg ccgcctgatc cgccgggtta tggctgaagt
    50881 ctgcgagggc ggcaacatcg cgagcgctgt aaggcgtttc cagttcaatc gtggttttcg
    50941 cccacttcga catcactaga tgagtctcga cattcggcat ctcccgcagc gcttgcagta
    51001 atgccacacc aagaggcgca ccggtagccc ctgtcatccc gacgatcagt ttcattgctt
    51061 actctcttaa ttgttcgtat acgaacgtta ttattaacat actccttcat ttccacagcg
    51121 gcaaggattg aaaagtaaca acatcttctt cgtcactgaa aaaccgctat gatagcgaca
    51181 gagaattagc ccggagttta catggcgtta cgaaataaag cgttccatca gttacgacag
    51241 ctttttcagc agcatacagc tcgctggcag cacgagttac ctgacctgac caaaccacag
    51301 tacgcggtga tgcgcgccat tgctgataag cctggtatcg aacaggtggc actgatagaa
    51361 gcagcagtca gcaccaaagc aacgctggca gaaatgttgg caagaatgga aaatcgtggc
    51421 ttagtcagac gtgaacatga cgccgctgac aagcggcgac gctttgtctg gctgactgcc
    51481 gaaggagaaa aggtacttgc tgcggcgatc cccattggtg acagtgtgga tgaggaattt
    51541 ttggggcgtt tgagtgcaga agagcaggag ctgtttatgc agttggtgcg caagatgatg
    51601 agcaaatagg atgctgattg ccgggtagga cgctgacgag tcttatccag gcgacaaaac
    51661 acaccgactt cccaaacaaa agaaaaaggc cagcctcgct tgagactggc ctttctgaca
    51721 gatgcttact tattcgcgga acagcgcttc gatattcagc ccctgcgttt gcaggatttc
    51781 gcgcaaacgg cgcaggcctt caacctgaat ctggcgaaca cgttcacggg tgaggccaat
    51841 ttcacgacct acatcttcca gtgttgccgc ttcgtacccc agcaaaccga atcgacgtgc
    51901 cagtacttca cgctgtttgg cgttcagctc gaacagccat ttgacgatgc tctgcttcat
    51961 atcgtcatct tgcgtggtat cttccggacc gttctctttt tcatcggcca ggatgtccag
    52021 caacgctttt tcggaatcac cacccagcgg ggtgtctacc gaggtaatgc gctcgttaag
    52081 acgaagcata cggctgacgt catcaactgg cttatccagt tgctctgcga tctcttccgc
    52141 acttggttcg tggtccagtt tatgggacaa ctcgcgtgcg gttcgcaggt aaacgttcag
    52201 ctcctttacg atgtgaatag gcaaacgaat agtacgggtt tggttcataa tcgcccgttc
    52261 gatcgtctgg cgaatccacc aggttgcgta tgttgagaag cggaaaccac gttccgggtc
    52321 aaacttctct accgcgcgga tcagccccag gttgccctct tcgatcaggt ccagcaacgc
    52381 cagaccacga ttgccataac ggcgggcaat ttttaccacc agacgcaagt tactctcgat
    52441 catccggcgg cgggaggcaa catctccacg cagtgcgcga cgcgcaaaat aaacttcttc
    52501 ttcggccgtt aacagtggtg aataaccaat ctcaccaagg taaagctgag tcgcgtccag
    52561 cacacgctgt gtggctccct gcgataacag ttcctcttcg gccaaatcgt tatcactggg
    52621 ttcctcttct actaaggcct tttcgtcaaa aacctcaact ccgttctcat caaattccgc
    52681 atcttcattt aaatcatgaa ctttcagcgt attctgactc ataaggtggc tcctacccgt
    52741 gatcccttga cggaacattc aagcaaaagc ctggttccgc cgatttatcg ctgcggcaaa
    52801 taacgcagcg ggtttacgga tttccccttg taacgaattt caaaatgcaa gcgtgttgaa
    52861 ctggttccgg tgctacccat ggttgctatt ttttgccccg ccttcacttc ttgttgttcc
    52921 cggaccagca ttgtgtcgtt atgggcgtag gcactcaggt aatcatcatt atgtttgatg
    52981 ataatcagat taccgtagcc gcgcagcgcg ttaccggcat aaacaacgcg gccatctgcg
    53041 gtcgcgataa ttgcctgtcc tttgctgcct gcgatatcaa tccccttgtt gcccccctca
    53101 gaagcgccaa aggtttcgat cactttgccc tcagtcggcc agcgccaggt ggagataggc
    53161 gtactggttg atgtactgct gacagtcggc tcggttgtgc ttgctgttgg taccgttaca
    53221 ggcgctgtga ccgtggtcgc agttggcttg ttgttcggca acattttgtt agcactctgt
    53281 tcacccgaag actcagaata cgtaattgtc ggttgcgacg caacagcaac ggtggaattt
    53341 tgtgcaggct tgatcacaac tccttgctct gctgcgtcgg cctgggtaat ggcatttccg
    53401 ccagtgattg gcgtaccgga agcattaccc acctgtaagg tctgaccgac gttcagcgcg
    53461 tatggtgcct gaatattgtt gcgctgagca aggtcacgga aatcgttgcc agtaatccag
    53521 gcgatataga aaagtgtgtc gccttttttc acggtatagg tactgccgct ataactgcct
    53581 ttcggaatgt tcccatactg acggttgtag acgatgcgtc cgttttccat ctgtaccggc
    53641 tgctgagcta ctggctgtac tggctggatt tgcggttgtt gagtagcctg aatttgtggc
    53701 tgctgtaccg gctgaatttg cggttgctgc gctgtagacg tcgtccccat tttcggcggc
    53761 ggcgtaatca acataccaga attggtattt gcaggcgcat tgccattaac ggagctgacc
    53821 ggtgccggtg gatttgaagt gtcagaacag cctgccagcc atagcgaaac cagtgacaaa
    53881 gccgcaatgc ggcgaacggt gaattttggg cttcccgcgc tcatttatcc cccaggaaaa
    53941 attggttaat aaccagtgac ataattaccg tgcaaggcac catactgaac actggaaaag
    54001 atgttcacga tacgctgacc tgcggcaaaa taaccaggaa aaatccaggt atttcctcac
    54061 gttttaagcc agctcaccct tcactaaagg gacaaagcgc acggcctcca cggtatcgat
    54121 aataaattcg cctccccgac gacgcacccg tttcaaatac tggtgctcct cccctacggg
    54181 taagacgaga attccgcctt cgtccagctg cgtcattagc gcagttggaa tttccggcgg
    54241 tgccgccgta acaatgatag cgtcaaacgg cgcacgtgcc tgccaacctt gccatccatc
    54301 gccatgacgg gttgaaacat tatgtaaatc aagatttttc aggcggcgac gcgcctgcca
    54361 ctgcaagcct ttaatccgtt caaccgagca aacatgctgg acaagatgcg ccaggattgc
    54421 cgtttgatat cccgaaccgg tgccaatttc cagcacccgc gactgcggcg tcagttcgag
    54481 taattcggtc attcgcgcca ccatatacgg ctgcgaaatt gtctgcccct gacctatcgg
    54541 caaggcgata ttgtcccagg ctttttgttc aaacgcttca tcaacgaatt tttcacgcgg
    54601 cacggcggca agtgcattca gcaccagctc atcctgaata ccttgcgcac gtaattgatc
    54661 cagaagtgct tgtacgcgtc tgcttaccat tgcgtgccaa ctcccacgct gtttaaccag
    54721 tctgaaacca catcttgcgc gctatgcgca gttaaatcca catgcagcgg cgtgatggaa
    54781 acatagccct catctaccgc agcaaaatcg gtccccggac cggcatcaca tttaccgccc
    54841 ggcgggccaa tccagtacag cgtattgccg cgcggatctt gctgcgggat cacctgatct
    54901 gccggatgtc gtgtaccgca gcgcgtcacg cgaatacctt tgatttgatc caatggtaaa
    54961 tccggaacgt taatattaag aatacgtccg gtgcgcagcg gctctttaca cagtgcgcgc
    55021 aaaattgaac aggttaccgc cgcggcagtg tcgtaatgtt tatgcccgtc aagcgagacg
    55081 gccagcgccg gaaaacctaa atgacggcct tccatcgcgg cggctaccgt accggaataa
    55141 ataacatcat cccccagatt cggcccggcg ttaattccgg acacaacaat gtccgggcgc
    55201 ggacgcatca gagcattcac gccaagatag acgcaatcgg tcggggttcc catttgcaca
    55261 gcaatatcac cattttcaaa ggtaaacgtg cgcagggagg attccagtgt cagagaattt
    55321 gaagcgccgc tgcggttacg atcgggggcg accacctgaa cgtcagcaaa ctcacgcaag
    55381 gctttcgcca gcgtttgtat accgggtgca tgtaccccgt catcattact cagcaatatg
    55441 cgcataatca cctgttgtgt tgataagttc cctgacaacg ctggttgcaa aacttcccgc
    55501 cggaagccag aaacggattt ctacggtgac gtcatcccac cagttccagc ttaatttttg
    55561 cggatacagc agcatcgctc tgcgcgcggc ttcaactttt tcacgcacca gtaaagtttg
    55621 taattcagtt tcttcggcga cagctgcttg ttcgaatgcc agcgcttcac gctgagttcc
    55681 ccattcgcca ctgcccggca atgcggcggt tatcatcagc tctttatcgt tgacgcgacg
    55741 ctgtaattcc gccagttctt cggtggttgc aacaaaccag ctaccacgtc cggctaattg
    55801 tagcgcatcg ccgtcaacaa cttgattaac gtctgctttt ttgaggcgct cagcaacaat
    55861 ctgattaaac aacgcactgc gggctgccga caaccaaaaa ctccgtttat tgcgatcgcg
    55921 caccggagta ttggtttgcg cccaacgcag cgcgccctgc aagttgctac cgccaatccc
    55981 aaaacgttgg gcaccgaagt agttcggtac accttttacg caaatatcga tcagacgttg
    56041 ttcaacgtca tcgcgattgc tcacttcgcg tagtaccagg gtaaaggcgt tacctttcag
    56101 cgcccctaaa cgcagcttgc gcttgtgccg cgcatactcc agcacctggc agccttccag
    56161 ttgaaaggcg ctcagatcgg gcatctcttt gcccggcacg cgagcgcata accactgttc
    56221 cgtaacagca tgtttatctt tttgcccagc gaagctgact tcacgggcat gaattttcag
    56281 gaatttcgcc agtgcatccg ccacaaaacg ggtattgcag ccgtttttga ggattctaac
    56341 cagaatatgc tcaccttcac catcaggctc aaagcccaaa tcttccacca ccacaaagtc
    56401 ttccggattg gctttcagca gcccggtgcc ttgcggttta ccgtggaggt aagtgagatt
    56461 atcaaactca atcattttgt tgccttaatg agtagcgcca ccgcttcaca ggcaatccct
    56521 tccccacgtc cggtaaatcc cagtttttcc gtagtagtgg ctttcacgtt aacatcatcc
    56581 atatggcagc cgagatcttc ggcaataaac acgcgcattt gtggaatgtg cggcaacatc
    56641 ttcggtgcct gagcgatgat agtgacatcg acgttgccga gggcataacc tttcgcctga
    56701 atacgacgcc aggcttcgcg caacagctcg cggctgtcgg cacctttaaa tgccggatcg
    56761 gtatccggga acagcttgcc tatatctccc agcgccgccg cgccaagcaa tgcatcggtc
    56821 aacgcatgga gcgccacgtc gccatcagaa tgcgccagca gccctttttc gtaaggaatg
    56881 cgtacgccac caatgataat tgggccttca ccgccaaagg cgtgtacgtc aaaaccgtgt
    56941 ccaattcgca ttatgtattc tcctgatgga tggttcgggt gaggtaaaat tcggccagcg
    57001 ccaaatcctc cgggcgcgtt accttgatgt tatccgcgcg gccttcaaca agttgcggat
    57061 gaaaaccgca atattccagc gccgaggcct catcggtaat ggtagcgcct tcatttagag
    57121 cgcgcgtcag gcagtcatgt aacagctcac gagggaaaaa ttgcggcgtc agcgcgtgcc
    57181 ataagccgtt gcgatcaacg gtatgagcaa tggcattttt gcccggttcg gcacgtttca
    57241 tcgtatcgcg caccggcgca gcgaggatcc cccccgtgcg gctggtttcg ctcaacgcca
    57301 acaatcgcgc gaggtcatcc tgatgcagac aaggacgagc ggcgtcatgc accaataccc
    57361 actgcgcgtc gccagcggct ttcagacctg ccagcacgga atcggcacgc tcttcaccac
    57421 catcaacaac cgtgattcgt ggatgattcg ccagaggaag ttgtgcaaag cggctatcgc
    57481 caggacttat ggcaatgacg acacgtttca ccctgggatg cgccagcagc gcatacaccg
    57541 agtgttcaag aatggtttga ttaccgattg agagatattg cttaggacat tccgtttgca
    57601 ttcggcggcc aaatccggcc gccggaacca cggcgcaaac atccaaatga gtggttgcca
    57661 tgttaattcc cgggctgatt tatcgattat tttgccccgc agactgtgcg cgctttgacg
    57721 cgtcaggcac cagacgataa aacgtttcgc ccggcctggt catgctgagt tcattacgcg
    57781 cacgctcttc gagcgcctcc tggccgccat tgagatcgtc aatttcggca aaaagctgat
    57841 cgtttcgcgc tttaagtttc gcgtttgtag cttgctgtgc cgccacatca ttattgacgc
    57901 gagtatagtc atgtataccg ttcttaccga accacagcga atactgtagc cagaccagaa
    57961 tagccagcaa cagcagcgtt agtttaccca tcctgccccc tgaaaaacgg catcttcatc
    58021 ccatgcatcc gaagacgact ctacatcctc tgttggggat accgcgacaa cgcgggcaaa
    58081 tgtaccacat ttgtccattg ttacgtatac ccagggcgtg cagaacataa tctcattatt
    58141 agttacggat tgaattatga acagaataga gaggaaagaa caattaaccc agtagccaca
    58201 taaacagcgc accaaacata atgccaaccg tcgccagagt gaaaacaata ctgtagcgta
    58261 gctttccgtc catcaatgaa tgcagtgcaa tccccaccac tacagcgacg ggcatcagcg
    58321 ccagaaagaa aggccaggtg tagataaaaa agagcagcgt gttagagcca taaatcaaca
    58381 tcggaattgc caacgcgcac agccacgata aaaaaccaac cacagcgcct ggcagcgacc
    58441 atgtggtgtc ttcaacctca gtaaggctgt cgttatttgt taatgtaatg ttgtggctgt
    58501 tacgcatatc tgatcctgtt actttgacga accgggcatg gaaacccggc ggtgtctcag
    58561 gatctgataa tatcgttctg tctcaacaga tctaataatt gctgtaccaa atttgttact
    58621 aattgttcac cattgagatg aatttctgcc gattcaggcg cttcgtaaac ggaatctatt
    58681 cccgtaaagt tgcgcagttc accggcacgc gctttcttat ataaaccttt tggatcgcgg
    58741 gcttcgcaaa tcgccagcgg cgtatcgaca aacacttcga taaagcgccc ttctcctacg
    58801 cgttcgcgaa ccatctggcg ttcggcgcgg tgtggcgaga taaatgcggt cagcaccacc
    58861 agtccggctt caaccatcaa attcgccact tcaccaaccc gacggatatt ctctttacgg
    58921 tcggcatcgc taaaaccgag atcgctgcat aatccgtggc gaacgttgtc gccatccagc
    58981 agatacgtac tgacgccaag tttatgtaac gcctcctcca gcgcccccgc gacggttgat
    59041 ttaccggaac cagagaggcc ggtaaaccac agcacaacac cacgatgacc gtggtgctgt
    59101 tcgcgttgtt gcggagttac tgggtggcta tgccagacga cgttttcgtc atgcagcgcc
    59161 attatttatc tcccagtaaa tcgcgcgcac cccaatgcgg gaaatggcgg cgaaccaggg
    59221 cattcaattc cagttcgaat gcactgaatt cagatggcgt agcagttgcc tggctaactg
    59281 gttcgtgcac cataccggca cctacggtca cattactcag gcgatcgata aaaatcagcc
    59341 caccggtaac cgggttttgc tgataacgat ctaacaccag tggctcgtca aaggtgagat
    59401 ctaccaggcc gatgccgttc agcggcaggt tttcaacttc gcgttgggta aggttattga
    59461 tatcaacctg ataacgaatg ccatcaacac gagcacgcgt cttcttaccg gcaattttga
    59521 tgtcataact ctggcccggg gaaagcggct gttccgccat ccataccaca tccaccgacg
    59581 cgctctgcac agccggtaac gcttcgtctg ccgccagcag cagatcgcca cggctgatgt
    59641 cgatctcatc cgtcagcacc agggtgatcg cttctccggc aaaggcttct tcgcgatcgc
    59701 catcaaaagt cacgatccgc gcgacgtttg attccacacc agagggcagc actttgacac
    59761 gttgcccgac ttccacgcga ccggatgcca gcgttccggc gtagccacga aaatcgagat
    59821 tcggacggtt aacgtactgc accgggaagc gcattggctg agcatccacc actcgctgga
    59881 tctccacggt ttccagcact tctagcagtg tcggaccgct gtaccacggc atactttcac
    59941 tttgcgaagc cacgttgtcg ccttccagtg cggagagcgg cacaaagcgg atatccagat
    60001 tacccggcag ctgcccggca aaagtcagat aatcttcacg aatacgggtg aacgtctctt
    60061 cactgtaatc caccagatcc attttgttga tcgccacgac cagatgtttg atccccaaca
    60121 gtgtggagat aaaactatga cgacgggttt gatcgagcac gcctttacgg gcatcgatca
    60181 gtaagatcgc cagttcacat gtcgatgcgc cagtcgccat attgcgggtg tactgctcgt
    60241 gccctggggt gtcggcgata ataaatttac gcttctcggt agagaaatag cggtaggcca
    60301 catcaatagt gatgccctgt tcgcgctcag cttgcaggcc gtccaccagc agtgccagat
    60361 ccagcttttc gccctgggtg ccgtgacgct tactgtcgtt atgcagcgat gagagctgat
    60421 cttcgtagat ttggcgggta tcgtgcagca ggcgaccaat cagggtactt ttgccgtcat
    60481 cgacgctacc acaggtcaga aaacgcagca ggcttttatg ttgttgcgca atcatccatg
    60541 cttcgacgcc gccttcattg gcgatttgtt gtgcaagtgc ggtgttcatc ttaaaaatac
    60601 ccctgacgtt ttttcagctc catagacccc gcctggtcgc ggtcaattac gcggccctga
    60661 cgttcactgg tggtggaaac cagcatctct tcgatgatct ccggcagtgt ttgcgcattt
    60721 gactccaccg cgccggtcag cggccaacag cccagtgtgc ggaaacgcac catccgtttt
    60781 ttaatcactt cgcccggttg caggtcgata cggttgtcat caatcatcat caacataccg
    60841 tcgcgttcca gaaccggacg ttccgcagca agatacagcg gaacaatgtc gatattttcc
    60901 agccagatgt attgccagat atcctgctcg gtccagttag agagcgggaa gacgcggatg
    60961 ctttcgcctt tgttaatttg cccgttgtag ttgtgccaca gctccgggcg ctgatttttc
    61021 ggatcccagc gatggaagcg gtcacggaaa gagtagattc gctctttagc gcgggatttc
    61081 tcttcgtcac ggcgcgcacc accgaaggcg gcatcaaaac cgtatttgtt cagcgcctgt
    61141 ttcaggcctt cagttttcat aatatcggta tgtttcgcgc tgccgtgtac gaatggatta
    61201 atccccatcg ccacgccttc cgggttttta tgcaccagca gctcgcagcc gtaggctttc
    61261 gccgtacgat cgcggaactc atacatctcg cggaatttcc agccggtatc gacatgcagc
    61321 aacgggaaag gcagcgtacc tggataaaac gccttgcgcg ccagatgcag catgacgctg
    61381 gaatctttac cgatagagta gagcatcacc ggatttgaga attctgcagc cacctcgcga
    61441 ataatgtgga tgctttccgc ctccagttgc cgcaggtggg taagtcgttt ttgatccata
    61501 accgttcctt tgcaataccg ctattttctt gccatcagat gtttcgacta tagggagcgt
    61561 aagagaacga atgaaattac caattagaat gagtagttcc ttaacggaat aacgatttgg
    61621 caaagctaat atcaaaaagt gcttaaggca ccggatttcg ggcgtttagg aagatttgaa
    61681 attgttttag cgcagcggca gtttcatact atgacggtaa aaaaatttgc atggtattta
    61741 aggactcact atgttttccg cattgcgcca ccgtaccgct gccctggcgc tcggcgtatg
    61801 ctttatcctc cccgtacacg cctcgtcacc taaacctggc gattttgcta atacccaggc
    61861 acgacatatt gctactttct ttccgggacg catgaccgga actcctgcag aaatgttatc
    61921 tgccgattat attcgccaac agtttcagca aatgggttat cgcagtgata ttcggacatt
    61981 taatagtcgg tatatttata ccgcccgcga taatcgcaag agctggcata acgtgacggg
    62041 aagtacggtg attgccgctc atgaaggcaa agctccgcag cagatcatca ttatggcgca
    62101 tctggatact tacgctccgc tgagcgatgc tgacgccgat gccaatctcg gcgggctgac
    62161 gttacaagga atagatgata acgccgcagg tttaggtgtc atgctggaac tggcagaacg
    62221 cctgaaaaat acgcctaccg agtatggtat tcgatttgtg gcgaccagtg gtgaagagga
    62281 agggaaatta ggtgctgaga atttactcaa gcggatgagt gacaccgaaa agaaaaatac
    62341 gctgctggtg attaatctcg ataacttaat tgttggcgat aaattgtatt tcaatagtgg
    62401 tgtaaaaacc cctgaagcag taaggaaatt aacgcgcgac agggcgctgg caattgcgcg
    62461 tagtcatgga attgccgcaa cgaccaatcc gggtttgaat aaaaattatc cgaaaggcac
    62521 tgggtgttgt aatgacgcag aaatattcga caaagccggt attgctgtac tttcggtgga
    62581 agcgactaac tggaatcttg ggaataagga tggttatcag caacgcgcaa aaacagccgc
    62641 attccctgcg ggaaatagct ggcatgacgt aagactggat aatcaacaac atattgataa
    62701 ggcgcttcct ggaagaatag aacgtcgctg ccgtgatgtt atgcggataa tgctacctct
    62761 ggtgaaggag ttggcgaagg cgtcttgatg ggtttgaaaa tgggagttgg gagttctacc
    62821 gcaggggcgg ggaattctaa gtgatatcca tcatcgcatc cagtgcgccc ggtttatccc
    62881 cgctgacgcg gggaacacac atgaatgtcg gttcagaccg tgtttttacc cggtttatcc
    62941 ccgctggcgc ggggaacacg aggctgaaca gggagggggc taccggcaca gcggtttatc
    63001 cccgctggcg cggggaacac ttgccaaccg ccaaagactg gaaaaccaat ctcggtttat
    63061 ccccgctggc gcggggaaca cgaactggcg ttgagtgata aatttgacgg aatcggttta
    63121 tccccgctgg cgcggggaac tcttcagttt cacactcggt aatgccgtta tttacggttt
    63181 atccccgctg gcgcggggaa ctcctcgggg ccagcctccc ccattaaccc ggcaccggtt
    63241 tatccccgct ggcgcgggga actctctaaa agtatacatt tgttcttaaa gcatttttt
//
LOCUS       DAZFLD010000004        62791 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.004, whole genome shotgun sequence.
ACCESSION   DAZFLD010000004 DAZFLD010000000
VERSION     DAZFLD010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 62791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 62791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..62791
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.004"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..162
                     /gene="yhfL"
                     /locus_tag="ACHKBL_000201"
     CDS             <1..162
                     /gene="yhfL"
                     /locus_tag="ACHKBL_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfL family protein"
                     /protein_id="HFO5817129.1"
                     /translation="KFIKVALVGAVLATLTACTGHIENRDKNCSYDYLLHPAISISKI
                     IGGCGPTAQ"
     gene            complement(269..1354)
                     /gene="yhfS"
                     /locus_tag="ACHKBL_000202"
     CDS             complement(269..1354)
                     /gene="yhfS"
                     /locus_tag="ACHKBL_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFO5817130.1"
                     /translation="MKTFPLQSLTLIEAQQKQFALVDTICRHFPGSEFLTCGDLGLTP
                     GLNQPRITQRVEQVLADAFHAPAAALVQGAGTGAIRAALAALLKPGQRLLVHDAPVYP
                     TTRVIIEQMGLTLITADFNDLSALKQVVDEQQPDAALVQHTRQQPQDRYVLADVLATL
                     RAAGVPALTDDNYAVMKVSRIGCECGANVSTFSCFKLFGPEGVGAVVGDADVISRIRA
                     TLYSGGSQIQGAQALEVLRGLVLAPVMHAVQAGVSERLLALLNGGAVAEVKSAVIANA
                     QSKVLIVEFHQPIAARVLEEAQKRGALPYPVGAESKYEIPPLFYRLSGTFRQANPQLE
                     HYAIRINPNRSGEETVLRILRESIASI"
     gene            complement(1366..2670)
                     /gene="yhfT"
                     /locus_tag="ACHKBL_000203"
     CDS             complement(1366..2670)
                     /gene="yhfT"
                     /locus_tag="ACHKBL_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfT family protein"
                     /protein_id="HFO5817131.1"
                     /translation="MDLYIQIIVVACLTGMTSLLAHRSAAVFHDGIRPILPQLIEGYM
                     NRREAGSIAFGLSIGFVASVGISFTLKTGLLNAWLLFLPTDILGVLAINSLMAFGLGA
                     IWGVLILTCLLPVNQLLTALPVDVLGSLGELSSPVVSAFALFPLVAIFYQFGWKQSLI
                     AAVVVLMTRVVVVRYFPHLNPESIEIFIGMVMLLGIAITHDLRHRDENDIDASGLSVF
                     EERTSRIIKNLPYIAIVGALIAAVASMKIFAGSEVSIFTLEKAYSAGVTPEQSQTLIN
                     QAALAEFMRGLGFVPLIATTALATGVYAVAGFTFVYAVGYLSPNPMVAAVLGAVVISA
                     EVLLLRSIGKWLGRYPSVRNASDNIRNAMNMLMEVALLVGSIFAAIKMAGYTGFSIAV
                     AIYFLNESLGRPVQKMAAPVVAVMITGILLNVLYWLGLFVPA"
     gene            complement(2682..3035)
                     /gene="yhfU"
                     /locus_tag="ACHKBL_000204"
     CDS             complement(2682..3035)
                     /gene="yhfU"
                     /locus_tag="ACHKBL_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2620 domain-containing protein"
                     /protein_id="HFO5817132.1"
                     /translation="MKKIGVAGLQREQIKKTIEATAPGCFEVFIHNDMEAAMKVKSGQ
                     LDYYIGACNTGAGAALSIAIAVIGYNKSCTIAKPGIKAKDEHIAKMIAEGKVAFGLSV
                     EHVEHAIPMLINHLK"
     gene            complement(3046..3924)
                     /gene="php"
                     /locus_tag="ACHKBL_000205"
     CDS             complement(3046..3924)
                     /gene="php"
                     /locus_tag="ACHKBL_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
                     /protein_id="HFO5817133.1"
                     /translation="MSFDPTGYTLAHEHLHIDLSGFKNNVDCRLDQYAFICQEMNDLM
                     AQGVRNVIEMTNRYMGRNAQFMLDVMRETGINVVACTGYYQDAFFPEHVATRSVQELA
                     QEMVDEIEQGIDGTELKAGIIAEIGSSEGKITPLEEKVFIAAALAHNQTGRPISTHTS
                     FSTMGLEQLALLQAHGVDLSRVTVGHCDLKDNLDNIVKMIDLGAYVQFDTIGKNSYYP
                     DEKRIAMLHALRDRGLLNRVMLSMDITRRSHLKANGGYGYDYLLTTFIPQLRQSGFSQ
                     ADVDVMLRETPSQFFQ"
     gene            complement(3921..5147)
                     /gene="yhfW"
                     /locus_tag="ACHKBL_000206"
     CDS             complement(3921..5147)
                     /gene="yhfW"
                     /locus_tag="ACHKBL_000206"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFO5817134.1"
                     /translation="MARFVVLVIDSFGVGAMKDVTLVRPQDAGANTCGHILSQLPHLQ
                     LPMLEKMGLINALGYAPGDMQPSDSATWGVAELQHEGGDTFMGHQEILGTRPLPPLRM
                     PFRDVIDRVEQALLNADWQVERRGDDLQFLWVNQAVAIGDNLEADLGQVYNITANLSV
                     ISFDDAIKIGRIVREQVQVGRVITFGGLLTDSQRILDAAESKEGRFIGINAPRSGAYD
                     NGFQVVHMGYGVDEKVQVPQKLYEVGVPTVLVGKVADIASNPYGVSWQNLVDSQRIMD
                     ITFNEFNTHPTAFICTNIQETDLAGHAEDVARYAERLQVVDRNLARLIEAMQPDDCLV
                     VMADHGNDPTIGHSHHTREVVPVLVYQQGMVATQLGVLTTLSDVGATVCEFFRAPPPQ
                     NGRSFLSSLRFAGDTL"
     gene            complement(5147..6310)
                     /gene="yhfX"
                     /locus_tag="ACHKBL_000207"
     CDS             complement(5147..6310)
                     /gene="yhfX"
                     /locus_tag="ACHKBL_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfX family PLP-dependent enzyme"
                     /protein_id="HFO5817135.1"
                     /translation="MFVEALKRQNPALISAALSLWQQGKIAPDSWVIDVDQILENGKR
                     LIETARLYGIELYLMTKQFGRNPWLAEKLLALGYSGIVAVDYKEARVMRRAGLPLAHQ
                     GHLVQIPCHQVSDAVAQGTDVITVFTLDKAREVSAAAVKAGRVQSVLLKVYSDDDFLY
                     PGQESGFVQHSLHEVVADIKKLPGLHLAGLTHFPCLLWDEAAGKVLPTPNLHTLIQAR
                     DQLAKSGIAIEQLNAPSATSCTSLPLLAEYGVTHAEPGHALTGTIPANQQGDQPERIA
                     MLWLSEISHHFRGDSYCYGGGYYRRGHAQHALVFTPENQKITETNLKTVDDSSIDYTL
                     PLAGEFPVSSAVVLCFRTQIFVTRSDVVLLSGIHRGEPEIVGRYDSLGNSLGA"
     gene            complement(6394..6756)
                     /gene="yhfY"
                     /locus_tag="ACHKBL_000208"
     CDS             complement(6394..6756)
                     /gene="yhfY"
                     /locus_tag="ACHKBL_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFO5817136.1"
                     /translation="METRLNLLCEAGVIDKDICKGMMQVVNVLETECHLPVRSEQGMM
                     AMTHMASALMRSRRGEEIEPLDDELLAELAQSSHWQAVVQLHQVLLKEFALEVKPCEE
                     GYLLANLYGLWMAANEEV"
     gene            complement(6773..7678)
                     /gene="yhfZ"
                     /locus_tag="ACHKBL_000209"
     CDS             complement(6773..7678)
                     /gene="yhfZ"
                     /locus_tag="ACHKBL_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator YhfZ"
                     /protein_id="HFO5817137.1"
                     /translation="MRRTFIKKEGVVITTLARYLLGEKCGNRLKTIDELATECRSSVG
                     LTQAALKTLESSGAIRIERRGRNGSYLVEMDNKALLSHVDINNVVCAMPLPYTRLYEG
                     LASGLKAQFDGIPFYYAHMRGADIRVECLLNGVYDMAVVSRLAAESYLTQKGLCLALE
                     LGPHTYVGEHQLICRKGESANVNRVGLDNRSADQKIMTDVFFGDSDVERVDLSYHESL
                     QRIVKGDVDAVIWNVVAENELTMLGLEATPLTDDPRFLQATEAVVLTRVDDYPMQQLL
                     RAVVDKQALLAHQQRVVSGEQEPSY"
     gene            complement(7968..8972)
                     /gene="trpS"
                     /locus_tag="ACHKBL_000210"
     CDS             complement(7968..8972)
                     /gene="trpS"
                     /locus_tag="ACHKBL_000210"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HFO5817138.1"
                     /translation="MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCIYCIVDQ
                     HAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFG
                     ELSRMTQFKDKSARYAENINAGLFDYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
                     AQRFNALYGEIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVK
                     KIKRAVTDSDEPPVVRYDVQNKAGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGE
                     VADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASAHASRTLKAVYEAIGFVAKP
                     "
     gene            complement(8965..9723)
                     /gene="gph"
                     /locus_tag="ACHKBL_000211"
     CDS             complement(8965..9723)
                     /gene="gph"
                     /locus_tag="ACHKBL_000211"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008967 - phosphoglycolate phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HFO5817139.1"
                     /translation="MNKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPVAGEE
                     RVITWIGNGADILMERALTWARQERATLRKTMGKPPVDDDIPAEEQVRILRKLFDRYY
                     GEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
                     DDVQNKKPHPDPLLLVAERMGIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGE
                     AIDLSQPDVIYQSINDLLPALGLPHSENQESKND"
     gene            complement(9716..10393)
                     /gene="rpe"
                     /locus_tag="ACHKBL_000212"
     CDS             complement(9716..10393)
                     /gene="rpe"
                     /locus_tag="ACHKBL_000212"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFO5817140.1"
                     /translation="MKQYLIAPSILSADFARLGEDTAKALAAGADVVHFDVMDNHYVP
                     NLTIGPMVLKSLRNYGITAPIDVHLMVKPVDRIVPDFAAAGASIITFHPEASEHVDRT
                     LQLIKENGCKAGLVFNPATPLSYLDYVMDKLDVILLMSVNPGFGGQSFIPQTLDKLRE
                     VRRRIDESGFDIRLEVDGGVKVNNIGEIAAAGADMFVAGSAIFDQPDYKKVIDEMRSE
                     LAKVSHE"
     gene            complement(10411..11247)
                     /gene="dam"
                     /locus_tag="ACHKBL_000213"
     CDS             complement(10411..11247)
                     /gene="dam"
                     /locus_tag="ACHKBL_000213"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HFO5817141.1"
                     /translation="MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLN
                     TDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQ
                     DPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKPYFPEAELYHFAEKAQNAFF
                     YCESYADSMARADDASVVYCDPPYAPLSATANFTAYHTNNFTLEQQAHLAEIAEGLVD
                     RHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPA
                     KK"
     gene            complement(11354..12640)
                     /gene="damX"
                     /locus_tag="ACHKBL_000214"
     CDS             complement(11354..12640)
                     /gene="damX"
                     /locus_tag="ACHKBL_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_process: GO:0032506 - cytokinetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DamX"
                     /protein_id="HFO5817142.1"
                     /translation="MDEFKPEDELKPDPSDRRTGRSRQSSERSERTERGEPQINFDDI
                     ELDDTDDRRPTRAQKERNEEPELEEEIDESEDETVDEERVERRPRKRKKAASKPASRQ
                     YMMMGVGILVLLLLIIGIGSALKAPSTSSSDQTASGEKSIDLAGNATDQANGVQPAPG
                     TTSAENTQQDVSLPPISSTPTQGQTPAATDGQQRVEVQGDLNNALTQPQNQQQLNNVA
                     VNSTLPTEPATVAPVRNGNASRDTAKTQTAERPATTRPARQQAVIEPKKPQATVKTEP
                     KPVAQTPKRTEPAAPVASTKAPAATSTPAPKETATTAPVQTASPAQTTATPAAGGKTA
                     GNVGSLKSAPSSHYTLQLSSSSNYDNLNGWAKKENLKNYVVYETTRNGQPWYVLVSGV
                     YASKEEAKKAVSTLPADVQAKNPWAKPLRQVQADLK"
     gene            complement(12732..13820)
                     /gene="aroB"
                     /locus_tag="ACHKBL_000215"
     CDS             complement(12732..13820)
                     /gene="aroB"
                     /locus_tag="ACHKBL_000215"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HFO5817143.1"
                     /translation="MERIVVTLGERSYPITIASGLFNEPASFLPLKSGEQVMLVTNET
                     LAPLYLDKVRGVLEQAGVNVDSVILPDGEQYKSLAVLDTVFTALLQKPHGRDTTLVAL
                     GGGVVGDLTGFAAASYQRGVRFIQVPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     ASVVVDLDCLKTLPPRELASGLAEVIKYGIILDGAFFNWLEENLDALLRLDGPAMAYC
                     IRRCCELKAEVVAADERETGLRALLNLGHTFGHAIEAEMGYGNWLHGEAVAAGMVMAA
                     RTSERLGQFSSAETQRIITLLTRAGLPVNGPREMSAQAYLPHMLRDKKVLAGEMRLIL
                     PLAIGKSEVRSGVSHELVLNAIADCQSA"
     gene            complement(13877..14398)
                     /gene="aroK"
                     /locus_tag="ACHKBL_000216"
     CDS             complement(13877..14398)
                     /gene="aroK"
                     /locus_tag="ACHKBL_000216"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HFO5817144.1"
                     /translation="MAEKRNIFLVGPMGAGKSTIGRQLAQQLNMEFYDSDQEIEKRTG
                     ADVGWVFDLEGEEGFRDREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVY
                     LETTIEKQLARTQRDKKRPLLHVETPPREVLEALANERNPLYEEIADVTIRTDDQSAK
                     VVANQIIHMLESN"
     gene            complement(14799..16037)
                     /gene="hofQ"
                     /locus_tag="ACHKBL_000217"
     CDS             complement(14799..16037)
                     /gene="hofQ"
                     /locus_tag="ACHKBL_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA uptake porin HofQ"
                     /protein_id="HFO5817145.1"
                     /translation="MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQE
                     KLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGLITRQEGNILSVHSVAWQNDNIA
                     RQEAEQARAQANLPLENRSITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
                     KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQQAGGVGQVTT
                     LGSDLSVATATTHVGFNIGRINGRLLDLELSALEQKQQLDIIASPRLLASHLQPASIK
                     QGSEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKLHISQNVPGQVLQQA
                     DGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDG
                     KEDERRELVVFITPRLVSSE"
     gene            complement(15949..16353)
                     /gene="hofP"
                     /locus_tag="ACHKBL_000218"
     CDS             complement(15949..16353)
                     /gene="hofP"
                     /locus_tag="ACHKBL_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofP"
                     /protein_id="HFO5817146.1"
                     /translation="MRVKRWLLAGITLCLLTGMRDPFKPPEDLCRISELSQWRYQGMV
                     GRGERIIGVIKDGQKKWRRVQQNDVLENGWTILQLTPDALTLGTGTNCEPPHWLWQRQ
                     GDTNEAMDSRTTVDAYTRRTGGKAAKSDADGG"
     gene            complement(16343..16783)
                     /gene="hofO"
                     /locus_tag="ACHKBL_000219"
     CDS             complement(16343..16783)
                     /gene="hofO"
                     /locus_tag="ACHKBL_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofO"
                     /protein_id="HFO5817147.1"
                     /translation="MDMLFDWWFATSPRLRQLCWAVWLLMLVTLIFLSSTHHEESDAL
                     IRLRASHHQQWAALYRLVDTTPFSEEKTLPFSPLDFQLSGAQLVYWHPSAQGGELALK
                     TLWEAVPSAFTRLAERNVSVSRFSLSVEGDDLLFTLQLETPHEG"
     gene            complement(16767..17306)
                     /gene="hofN"
                     /locus_tag="ACHKBL_000220"
     CDS             complement(16767..17306)
                     /gene="hofN"
                     /locus_tag="ACHKBL_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofN"
                     /protein_id="HFO5817148.1"
                     /translation="MNPPINFLPWRQQRRTAFLRFWLLMFVAPLLLAVGITLILRLTS
                     NAEARVDTVLLQAEQQLARSLQITKPRLLERQQLREQRLQRQLQRQFTRDWQSALEAL
                     AALLPEHAWLTTISWQQGTLEIKGLTTSITALNALETSLRQDASFHLNQRGATQQDAQ
                     GRWQFEYQLTRKVSDGHAL"
     gene            complement(17306..18075)
                     /gene="hofM"
                     /locus_tag="ACHKBL_000221"
                     /pseudo
     CDS             complement(17306..18075)
                     /gene="hofM"
                     /locus_tag="ACHKBL_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417854.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofM"
     gene            18195..20747
                     /gene="mrcA"
                     /locus_tag="ACHKBL_000222"
     CDS             18195..20747
                     /gene="mrcA"
                     /locus_tag="ACHKBL_000222"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase/peptidoglycan
                     DD-transpeptidase MrcA"
                     /protein_id="HFO5817149.1"
                     /translation="MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVATLKDVRL
                     QIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVKAFIATEDSRFYEHHGVDPVGIF
                     RAASVALFSGHASQGASTITQQLARNFFLSPERTLMRKIKEVFLAIRIEQLLTKDEIL
                     ELYLNKIYLGYRAYGVGAAAQVYFGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRA
                     VARRNVVLSRMLDEGYITQQQFDQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRY
                     GESAYEDGYRIYTTITRKVQQAAQQAVRNNVLDYDMRHGYRGPANVLWKVGESAWDNN
                     KITDTLKALPTYGPLLPAAVTSANPQEATAMLADGSTVALSMEGVRWARPYRSDTQQG
                     PTPRKVTDVLQTGQQIWVRQVGDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQ
                     SKFNRATQALRQVGSNIKPFLYTAAMDKGLTLASMLNDVPISRWDAGAGSDWQPKNSP
                     PQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDYAAEYLQRFGFPAQNIVHTESLALGSA
                     SFTPMQVARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDT
                     QKSNVLENNDVEDVAISREQQNVSVPMPQLEQANQALVAKTGAQEYAPHVINTPLAFL
                     IKSALNTNIFGEPGWQGTGWRAGRDLQRHDIGGKTGTTNSSKDAWFSGYGPGVVTSVW
                     IGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPG
                     IVTVNIDRSTGQLANGGNSREEYFIEGTQPTQQAVHEVGTTIIDNGEAQELF"
     gene            complement(20915..21475)
                     /gene="nudE"
                     /locus_tag="ACHKBL_000223"
     CDS             complement(20915..21475)
                     /gene="nudE"
                     /locus_tag="ACHKBL_000223"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HFO5817150.1"
                     /translation="MSKSLQKPTILNVETVARSRLFTVESVDLEFSNGVRRVYERMRP
                     TNREAVMIVPIVDDHLILIREYAVGTESYELGFSKGLIDPGESVFEAANRELKEEVGF
                     GANDLTFLKKLSMAPSYFSSKMNIVVAQDLYPESLEGDEPEPLPQVRWPLAHMMDLLE
                     DPDFNEARNVSALFLVREWLKGQGRL"
     gene            21795..23930
                     /gene="igaA"
                     /locus_tag="ACHKBL_000224"
     CDS             21795..23930
                     /gene="igaA"
                     /locus_tag="ACHKBL_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular growth attenuator protein IgaA"
                     /protein_id="HFO5817151.1"
                     /translation="MSTIVIFLAALLACSLLAGWLIKVRSRRRQLPWTNAFADAQTRK
                     LTPEERSAVENYLESLTQVLQVPGPTGASAAPISLALNAESNNVMMLTHAITRYGIST
                     DDPNKWRYYLDSVEVHLPPFWEQYINDENTVELIHTDSLPLVISLNGHTLQEYMQETR
                     GYALQPVPSTQASIRGEESEQIELLNIRKETHEEYALSRPRGLREALLIVASFLMFFF
                     CLITPDVFVPWLAGGALLLLGAGLWGLFAPPAKSSLREIHCLRGTPRRWGLFGENDQE
                     QINNISLGIIDLVYPAHWQPYIAHDLGQQTDIDIYLDRHVVRQGRYLSLHDEVKNFPL
                     QHWLRSTIIAAGSLLVLFMLLFWIPLDMPLKFTLSWMKGAQTIEATSVKQLADAGVRV
                     GDTLRISGTGMCNIRTSGTWSAKTNSPFLPFDCSQIIWNDARSLPLPESELVNKATAL
                     TEAVNRQLHPKPEDESRVSASLRSAIQKSGMVLLDDFGDIVLKTADLCSAKDDCVRLK
                     NALVNLGNSKDWDALVKRANAGKLDGVNVLLRPVSAESLDNLVATSTAPFITHETARA
                     AQSLNSPAPGGFLIVSDEGSDFVDQPWPSASLYDYPPQEQWNAFQKLAQMLMHTPFNA
                     EGIVTKIFTDANGTQHIGLHPIPDRSGLWRYLSTTLLLLTMLGSAIYNGVQAWRRYQR
                     HRTRMMKIQAYYESCLNPQLITPSESLIE"
     gene            23995..24663
                     /gene="yrfG"
                     /locus_tag="ACHKBL_000225"
     CDS             23995..24663
                     /gene="yrfG"
                     /locus_tag="ACHKBL_000225"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HFO5817152.1"
                     /translation="MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWGAKNG
                     VTPQEAMEYMRQQYHDVQHTLNWYCLDYWSEQLGLDICAMTTEMGPRAVLREDTIPFL
                     EALKASGKQRILLTNAHPHNLAVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAE
                     ATGLKAERTLFIDDSEAILDAAAQFGIRYCLGVTNPDSGIAEKQYQRHPSLNDYRRLI
                     PSLM"
     gene            24674..25075
                     /gene="hslR"
                     /locus_tag="ACHKBL_000226"
     CDS             24674..25075
                     /gene="hslR"
                     /locus_tag="ACHKBL_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HFO5817153.1"
                     /translation="MKEKPAVEVRLDKWLWAARFYKTRALAREMIEGGKVHYNGQRSK
                     PSKIVELNATLTLRQGNDERTVIVKAITEQRRPASEAALLYEETAESVEKREKMALAR
                     KLNALTMPHPDRRPDKKERRDLLRFKHGDSE"
     gene            25100..25978
                     /gene="hslO"
                     /locus_tag="ACHKBL_000227"
     CDS             25100..25978
                     /gene="hslO"
                     /locus_tag="ACHKBL_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HFO5817154.1"
                     /translation="MPQHDQLHRYLFENFAVRGELVTVSETLQQILENHDYPQPVKNV
                     LAELLVATSLLTATLKFDGDITVQLQGDGPMNLAVINGNNNQQMRGVARVQGEIPENA
                     DLKTLVGNGYVVITITPSEGERYQGVVGLEGDTLAACLEDYFMRSEQLPTRLFIRTGD
                     VDGKPAAGGMLLQVMPAQNAQQDDFDHLATLTETIKTEELLTLPANEVLWRLYHEEEV
                     TVYDPQDVEFKCTCSRERCADALKTLPDEEVDSILAEDGEIDMHCDYCGNHYLFNAMD
                     IAEIRNNASPADPQVH"
     gene            complement(26116..27840)
                     /gene="yhgE"
                     /locus_tag="ACHKBL_000228"
     CDS             complement(26116..27840)
                     /gene="yhgE"
                     /locus_tag="ACHKBL_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HFO5817155.1"
                     /translation="MDNVELSPATRWGMIATGLLQGLVCYLLIAWLAGKNHSWIVYGV
                     PATVAFSSVLLFSVISFKQKRLWGWLALVFIATLGMSGWLKWQIDGMNPWRAEKALWD
                     FGCYLLLMAMLLLPWIQQSLRIRNDSSRYRYFYQSVWHNVLILLVIFLANGLTWLVLL
                     LWGELFKLVGITFFKTLFFATDWFIYLTLGLVTALAVILARTQSRLIDSIQKLFTLIA
                     TGLLPLVSLLTLMFIITLPFTGLSAISRHISAAGLLLTLAFLQLILMAIVRDPQKASL
                     PWTGPLRCLIKTALLVAPLYVFVAAWALWLRVAQYGWTVDRLQGALAVLVLLVWSLGY
                     FVSIVWRKGQNPLVLQGKVNLAVSLLVLVILVLLNSPVLDSMRISVNSHMARYQSGKN
                     TPDQVSLYMLEQSGRYGRAALESLKSDAEYMKDPKRRRGLLMAFDGVQRLQQQISEKA
                     LADNVFIASGSGKPDTIFWSAVMKNRYNAMICIEKDACVLVEQDLNSDGRAERILFAF
                     NDERYIIYGIDPAGKEWKEITMNSLPAVITKEKLLMAAEDGKLGTKPKVWRDLTVDGE
                     RLDLNLNE"
     gene            28219..29841
                     /gene="pckA"
                     /locus_tag="ACHKBL_000229"
     CDS             28219..29841
                     /gene="pckA"
                     /locus_tag="ACHKBL_000229"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HFO5817156.1"
                     /translation="MRVNNGLTPQELEAYGISDVHDIVYNPSYDLLYQEELDPSLTGY
                     ERGVLTNLGAVAVDTGIFTGRSPKDKYIVRDDTTRDTFWWADKGKGKNDNKPLSPETW
                     QHLKGLVTKQLSGKRLFVVDAFCGANPDTRLSVRFITEVAWQAHFVKNMFIRPSDEEL
                     AGFKPDFIVMNGAKCTNPQWKEQGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSM
                     MNYLLPLKGIASMHCSANVGEKGDVAVFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDD
                     GVFNFEGGCYAKTIKLSKEAEPEIYNAIRRDALLENVTVREDGTIDFDDGSKTENTRV
                     SYPIYHIENIVKPVSKAGHATKVIFLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLA
                     GTERGITEPTPTFSACFGAAFLSLHPTQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRI
                     SIKDTRAIIDAILNGSLDNAETFTLPMFNLAIPTELPGVDTRILDPRNTYASPEQWQE
                     KAETLAKLFIDNFDKYTDTPAGAALVAAGPKL"
     gene            29956..30273
                     /locus_tag="ACHKBL_000230"
     CDS             29956..30273
                     /locus_tag="ACHKBL_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO5817157.1"
                     /translation="MFTFIELQGFSKRRQLLLPDDEFRAFQELLIQDPTAGDIIAGTG
                     SFRKIRWNRSGMGKRGGIRIIYYNVTRNGRIYLALIYPKNEQDDLTEEQRKALKLLSE
                     KLL"
     gene            30332..30628
                     /gene="nadS"
                     /locus_tag="ACHKBL_000231"
     CDS             30332..30628
                     /gene="nadS"
                     /locus_tag="ACHKBL_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NadS family protein"
                     /protein_id="HFO5817158.1"
                     /translation="MKDELFADLLASAEEMVRIEKGEETPEPEHVHTFSEIDVKAIRE
                     ATGLRQQDFAVAVGVSYDLVKSWETKRRQPTGAPRKLLLLLQANPFIINQLKTI"
     gene            complement(30660..32012)
                     /gene="envZ"
                     /locus_tag="ACHKBL_000232"
     CDS             complement(30660..32012)
                     /gene="envZ"
                     /locus_tag="ACHKBL_000232"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     EnvZ"
                     /protein_id="HFO5817159.1"
                     /translation="MRRLRFSPRSSFARTLLLIVTLLFVSLVTTYLVVLNFAILPSLQ
                     QFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWA
                     QHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
                     FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTR
                     AFNHMAAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEE
                     CNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIEVKMHP
                     LSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVR
                     GDSARTISGTGLGLAIVQRIVDNHNGMLELGSSERGGLSIRAWLPVPVTRAQGTTKEG
                     "
     gene            complement(32009..32728)
                     /gene="ompR"
                     /locus_tag="ACHKBL_000233"
     CDS             complement(32009..32728)
                     /gene="ompR"
                     /locus_tag="ACHKBL_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HFO5817160.1"
                     /translation="MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLL
                     TRESFHLMVLDLMLPGEDGLSICRRLRSQSNPMPIIMVTAKGEEVDRIVGLEIGADDY
                     IPKPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAFGKFKLNLGTREMFREDEPMP
                     LTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRRMVEEDPAH
                     PRYIQTVWGLGYVFVPDGSKA"
     gene            32956..33432
                     /gene="greB"
                     /locus_tag="ACHKBL_000234"
     CDS             32956..33432
                     /gene="greB"
                     /locus_tag="ACHKBL_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HFO5817161.1"
                     /translation="MKTPLVTREGYEKLKQELNYLWREERPEVTKKVTWAASLGDRSE
                     NADYQYNKKRLREIDRRVRYLTKCMENLKIVDYSPQQEGKVFFGAWVEIENDDGVTHR
                     FRIVGYDEIFGRKDYISIDSPMARALLKKEVGDLAVVNTPAGEASWYVNAIEYVKP"
     gene            33529..35850
                     /gene="yhgF"
                     /locus_tag="ACHKBL_000235"
     CDS             33529..35850
                     /gene="yhgF"
                     /locus_tag="ACHKBL_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HFO5817162.1"
                     /translation="MMNDSFCRIIAGEIQARPEQVDAAVRLLDEGNTVPFIARYRKEI
                     TGGLDDTQLRNLETRLSYLRELEERRQAILKSISEQGKLTDELANAINATLSKTELED
                     LYLPYKPKRRTRGQIAIEAGLEPLADLLWSDPSHTPEVAAAQYIDADKGVADTKAALD
                     GARYILMERFAEDAALLAKVRDYLWKNAHLVSTVVNGKEEEGAKFRDYFDHHEPLSTV
                     PSHRALAMFRGRNEGVLQLSLNADPQFEEPPKESYCEQIIMDHLGLRLNNAPADSWRK
                     GVVSWTWRIKVLMHLETELMGTVRERAEDEAINVFARNLHDLLMAAPAGLRATMGLDP
                     GLRTGVKVAVVDATGKLVATDTIYPHTGQAAKAAMTVAALCEKHNVELVAIGNGTASR
                     ETERFYLDVQKQFPKVTAQKVIVSEAGASVYSASELAAQEFPDLDVSLRGAVSIARRL
                     QDPLAELVKIDPKSIGVGQYQHDVSQTQLARKLDAVVEDCVNAVGVDLNTASVPLLTR
                     VAGLTRMMAQNIVAWRDENGQFQNRQQLLKVSRLGPKAFEQCAGFLRINHGDNPLDAS
                     TVHPEAYPVVERILAATQQALKDLMGNSSELRNLKASDFTDEKFGVPTVTDIIKELEK
                     PGRDPRPEFKTAQFADGVETMNDLQPGMILEGAVTNVTNFGAFVDIGVHQDGLVHISS
                     LSNKFVEDPHTVVKAGDIVKVKVLEVDLQRKRIALTMRLDEQPGETNARRGGGNDRPQ
                     NNRPAAKPRGREAQPAGNSAMMDALAAAMGKKR"
     gene            36288..36515
                     /gene="feoA"
                     /locus_tag="ACHKBL_000236"
     CDS             36288..36515
                     /gene="feoA"
                     /locus_tag="ACHKBL_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter A"
                     /protein_id="HFO5817163.1"
                     /translation="MQYTPDTAWKITGFSREISPAYRQKLLSLGMLPGSSFNVVRVAP
                     LGDPIHIETRRVSLVLRKKDLALLEVEAVSC"
     gene            36532..38853
                     /gene="feoB"
                     /locus_tag="ACHKBL_000237"
     CDS             36532..38853
                     /gene="feoB"
                     /locus_tag="ACHKBL_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HFO5817164.1"
                     /translation="MKKLTIGLIGNPNSGKTTLFNQLTGSRQRVGNWAGVTVERKEGQ
                     FSTTDHQVTLVDLPGTYSLTTISSQTSLDEQIACHYILSGDADLLINVVDASNLERNL
                     YLTLQLLELGIPCIVALNMLDIAEKQNIRIEIDALSARLGCPVIPLVSTRGRGIEALK
                     LAIDRYKANENVELVHYAQPLLNEADSLAKVMPSDIPLKQRRWLGLQMLEGDIYSRAY
                     AGEASQHLDAALARLRNEMDDPALHIADARYQCIAAICDVVSNTLTAEPSRFTTAVDK
                     IVLNRFLGLPIFLFVMYLMFLLAINIGGALQPLFDVGSVALFVHGIQWIGYTLHFPDW
                     LTIFLAQGLGGGINTVLPLVPQIGMMYLFLSFLEDSGYMARAAFVMDRLMQALGLPGK
                     SFVPLIVGFGCNVPSVMGARTLDAPRERLMTIMMAPFMSCGARLAIFAVFAAAFFGQN
                     GALAVFSLYMLGIVMAVLTGLMLKYTIMRGEATPFVMELPVYHVPHVKSLIIQTWQRL
                     KGFVLRAGKVIIIVSIFLSAFNSFSLSGKIVDNINDSALASVSRVITPVFKPIGVHED
                     NWQATVGLFTGAMAKEVVVGTLNTLYTAENIQDEEFNPAEFNLGEELFSAVDETWQSL
                     KDTFSLSVLMNPIEASKGDGEMGTGAMGVMDQKFGSAAAAYSYLIFVLLYVPCISVMG
                     AIARESSRGWMGFSILWGLNIAYSLATLFYQVASYSQHPTYSLVCILAVILFNIVVIG
                     LLRRARSRVDIELLATRKSVSSCCAASTTGDCH"
     gene            38853..39089
                     /gene="feoC"
                     /locus_tag="ACHKBL_000238"
     CDS             38853..39089
                     /gene="feoC"
                     /locus_tag="ACHKBL_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-S]-dependent transcriptional repressor FeoC"
                     /protein_id="HFO5817165.1"
                     /translation="MASLIQVRDLLALRGRMEAAQISQTLNTPQPMINAMLQQLESMG
                     KAVRIQEEPDGCLSGNCKSCPEGKACLREWWALR"
     gene            39292..40173
                     /gene="rpnA"
                     /locus_tag="ACHKBL_000239"
     CDS             39292..40173
                     /gene="rpnA"
                     /locus_tag="ACHKBL_000239"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnA"
                     /protein_id="HFO5817166.1"
                     /translation="MSKKQSSTPHDALFKLFLRQPETARDFLAFHLPAPIHALCDMKT
                     LKLESSSFIDDDLRESYSDVLWSVKTEQGPGYIYCLIEHQSTSNKLIAFRMMRYAIAA
                     MQNHLDAGYKTLPMVVPLLFYHGIESPYPYSLCWLDCFADPKLARQLYASAFPLIDVT
                     VMPDDEIMQHRRMALLELIQKHIRQRDLMGLVEQMACLLSSGYANDRQIKGLFNYILQ
                     TGDAVRFNDFIDGVAEHSPKHKESLMTIAERLRQEGFQKGIRAIAKTMLDAGVPLEDV
                     LRFTSLTVEDLAEINHQ"
     gene            40245..40364
                     /locus_tag="ACHKBL_000240"
                     /pseudo
     CDS             40245..40364
                     /locus_tag="ACHKBL_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312280.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(40393..41163)
                     /gene="bioH"
                     /locus_tag="ACHKBL_000241"
     CDS             complement(40393..41163)
                     /gene="bioH"
                     /locus_tag="ACHKBL_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HFO5817167.1"
                     /translation="MNNIWWQTKGQGNVHLVLLHGWGLNAEVWRCIDEELSSHFTLHL
                     VDLPGFGRSRGFGAMSLADMAEAVLRQAPDKAIWLGWSLGGLVASQIALTHPERVQAL
                     VTVASSPCFSARDEWPGIKPDVLAGFQQQLSDDFQRTVERFLALQTMGTETARQDARA
                     LKKTVLALPMPEVDVLNGGLEILKMVDLRQPLQNVSMPFLRLYGYLDGLVPRKVVPML
                     DKLWPHSESYIFAKAAHAPFISHPAEFCRMLVALKQRV"
     gene            41201..41884
                     /gene="gntX"
                     /locus_tag="ACHKBL_000242"
     CDS             41201..41884
                     /gene="gntX"
                     /locus_tag="ACHKBL_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein GntX"
                     /protein_id="HFO5817168.1"
                     /translation="MLTVPGLCWLCRMPLALGHWGICSVCSRAARTDKTLCPQCGLPA
                     THSHLPCGRCLQKPPPWQRLVTVADYAPPLSPLIHQLKFSRRSEIASALARLLLLEVL
                     HARRTTGLQLPDRIISVPLWQQRHWRRGFNQSDLLCQPLSRWLHCRWDSEAVTRTRAT
                     ATQHFLSARLRKRNLKNAFRLELPVQGRHMVIVDDVVTTGSTVAEIAQLLLRNGAATV
                     QVWCLCRTL"
     gene            41943..42518
                     /gene="nfuA"
                     /locus_tag="ACHKBL_000243"
     CDS             41943..42518
                     /gene="nfuA"
                     /locus_tag="ACHKBL_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016942932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HFO5817169.1"
                     /translation="MIRISDAAQAHFAKLLANQEEGTQIRVFVINPGTPNAECGVSYC
                     PPDAVEATDTALKFDLLTAYVDELSAPYLEDAEIDFVTDQLGSQLTLKAPNAKMRKVA
                     DDAPLMERVEYMLQSQINPQLAGHGGRVSLMEITEDGYAILQFGGGCNGCSMVDVTLK
                     EGIEKQLLNEFPELKGVRDLTEHQRGEHSYY"
     gene            42879..44195
                     /gene="gntT"
                     /locus_tag="ACHKBL_000244"
     CDS             42879..44195
                     /gene="gntT"
                     /locus_tag="ACHKBL_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HFO5817170.1"
                     /translation="MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLD
                     KVIGSIKAGVGGTLGSLALIMGFGAMLGKMLADCGGAQRIATTLIAKFGKKHIQWAVV
                     LTGFTVGFALFYEVGFVLMLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGP
                     TAIATIFNADMGKTLLYGTILAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEE
                     MPSFGVSVWTSLVPVVLMAMRAIAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFT
                     FGLNRGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQVLVDSGVDKYIASMMHETNI
                     SPLLMAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSH
                     VNDPGFWLFKEYFNLTIGETIKSWSMLETIISVCGLVGCLLLNMVI"
     gene            complement(44240..46324)
                     /gene="malQ"
                     /locus_tag="ACHKBL_000245"
     CDS             complement(44240..46324)
                     /gene="malQ"
                     /locus_tag="ACHKBL_000245"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HFO5817171.1"
                     /translation="MESKRLDNAALAAGISPNYINAHGKPQSISAETKRRLLDAMHQR
                     TATKVAVTPVPNVMVYTSGKKMPMVVEGSGEYSWLLTTEEGTQYKGHVTGGKAFNLPT
                     KLPEGYHTLTFTQEDQRVHCRVIVAPKRCYEPQALLNKQKLWGACVQLYTLRSEKNWG
                     IGDFGDLKAMLVDVAKRGGSFIGLNPIHALYPANPESASPYSPSSRRWLNVIYIDVNA
                     VEDFHLSEEAQAWWKLPTTQQTLQQTRDADWVDYSTVTALKMTALRMAWKGFAQRDDE
                     QVAAFRQFVAEQGDSLFWQAAFDALHAQQVKEDEMRWGWPAWPEMYQNVDSPEVRQFC
                     EEHRDDVDFYLWLQWLAYSQFAACWEISQGYEMPIGLYRDLAVGVAEGGSETWCDREL
                     YCLKASVGAPPDILGPLGQNWGLPPMDPHIITARAYEPFIELLRANMQNCGALRIDHV
                     MSMLRLWWIPYGETADQGAYVHYPVDDLLSILALESKRHRCMVIGEDLGTVPVEIVGK
                     LRSSGVYSYKVLYFENDHEKTFRAPKAYPEQSMAVAATHDLPTLRGYWESGDLTLGKT
                     LGLYPDEVVLRGLYQDRELAKQGLLDALHKYGCLPKRAGHKASLMSMTPTLNRGLQRY
                     IADSNSALLGLQPEDWLDMADPVNIPGTSYQYKNWRRKLSATLEAMFADDGVNKLLKD
                     LDRRRRAAAKKK"
     gene            complement(46334..48727)
                     /gene="malP"
                     /locus_tag="ACHKBL_000246"
     CDS             complement(46334..48727)
                     /gene="malP"
                     /locus_tag="ACHKBL_000246"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HFO5817172.1"
                     /translation="MSQPIFNDKQFQEALSRQWQRYGLNSAAEMTPRQWWLAVSEALA
                     EMLRAQPFAKPVANQRHVNYISMEFLIGRLTGNNLLNLGWYQDVQDSLKAYDINLTDL
                     LEEEIDPALGNGGLGRLAACFLDSMATVGQSATGYGLNYQYGLFRQSFVDGKQVEAPD
                     DWHRSNYPWFRHNEALDVQVGIGGKVTKDGRWEPEFTITGQAWDLPVVGYRNGVAQPL
                     RLWQATHAHPFDLTKFNDGDFLRAEQQGINAEKLTKVLYPNDNHTAGKKLRLMQQYFQ
                     CACSVADILRRHHLAGRKLHELAEYEVIQLNDTHPTIAIPELLRVLIDEHQMSWDDAW
                     AITSKTFAYTNHTLMPEALERWDVKLVKGLLPRHMQIINEINTRFKTLVEKTWPGDEK
                     VWAKLAVVHDKQVHMANLCVVGGFAVNGVAALHSDLVVKDLFPEYHQLWPNKFHNVTN
                     GITPRRWIKQCNPALAALLDKSLKKEWTNDLDQLINLEKFADDAKFRQQYREIKQANK
                     VRLAEFVKVRTGIEINPQAIFDIQIKRLHEYKRQHLNLLHILALYKEIRENPQADRVP
                     RVFLFGAKAAPGYYLAKNIIFAINKVADVINNDPQVGDKLKVVFLPDYCVSAAEKLIP
                     AADISEQISTAGKEASGTGNMKLALNGALTVGTLDGANVEIAEKVGEENIFIFGHTVE
                     QVKAILAKGYDPVKWRKKDKVLDAVLKELESGKYSDGDKHAFDQMLHSIGKQGGDPYL
                     VMADFAAYVEAQKQVDVLYRDQEAWTRAAILNTARCGMFSSDRSIRDYQARIWQAKR"
     gene            49349..52054
                     /gene="malT"
                     /locus_tag="ACHKBL_000247"
     CDS             49349..52054
                     /gene="malT"
                     /locus_tag="ACHKBL_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MalT"
                     /protein_id="HFO5817173.1"
                     /translation="MLIPSKLSRPVRLDHTVVRERLLAKLSGANNFRLALITSPAGYG
                     KTTLISQWAAGKNDIGWYSLDEGDNQQERFASYLIAAVQQATNGHCAICETMAQKRQY
                     ASLTSLFAQLFIELAEWHSPLYLVIDDYHLITNPVIHESMRFFIRHQPENLTLVVLSR
                     NLPQLGIANLRVRDQLLEIGSQQLAFTHQEAKQFFDCRLSSPIEAAESSRICDDVSGW
                     ATALQLIALSARQNTHSAHKSARRLAGINASHLSDYLVDEVLDNVDLATRHFLLKSAI
                     LRSMNDALITRVTGEENGQMRLEEIERQGLFLQRMDDTGDWFCYHPLFGNFLRQRCQW
                     ELAAELPEIHRAAAESWMAQGFPSEAIHHALAAGDALMLRDILLNHAWSLFNHSELSL
                     LEESLKALPWDSLLENPQLVLLQAWLMQSQHRYGEVNTLLARAEHEIKDIREGTMHAE
                     FNALRAQVAINDGNPDEAERLAKLALEELPPGWFYSRIVATSVLGEVLHCKGELTRSL
                     ALMQQTEQMARQHDVWHYALWSLIQQSEILFAQGFLQTAWETQEKAFQLINEQHLEQL
                     PMHEFLVRIRAQLLWAWARLDEAEASARSGIEVLSSYQPQQQLQCLAMLIQCSLARGD
                     LDNARSQLNRLENLLGNGKYHSDWISNANKVRVIYWQMTSDKAAAANWLRHTAKPEFA
                     NNHFLQGQWHNIARAQILLGEFEPAEIVLEELNENARSLRLMSDLNRNLLLLNQLYWQ
                     AGRKSDAQRVLLDALKLANRTGFISHFVIEGEAMAQQLRQLIQLNTLPELEQHRAQRI
                     LREINQHHRHKFAHFDENFVERLLNHPEVPELIRTSPLTQREWQVLGLIYSGYSNEQI
                     AGELEVAATTIKTHIRNLYQKLGVAHRQAAVQHAQKLLKMMGYGV"
     gene            complement(52097..53113)
                     /gene="rtcA"
                     /locus_tag="ACHKBL_000248"
     CDS             complement(52097..53113)
                     /gene="rtcA"
                     /locus_tag="ACHKBL_000248"
                     /EC_number="6.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003963 - RNA-3'-phosphate cyclase
                     activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HFO5817174.1"
                     /translation="MKRMIALDGAQGEGGGQILRSALSLSMITGQPFTITGIRAGRAK
                     PGLLRQHLTAVKAAAEICRATVEGAELGSQRLVFRPGTVRGGDYRFAIGSAGSSTLVL
                     QTVLPALWFADGPSRVEVSGGTDNPSAPPADFIRRVLEPLLAKMGIHQQTTLLRHGFY
                     PAGGGVVATEVSPVALFNTLQLGERGNIVQMRGEVLLAGVPRHVAEREIATLAGSFSL
                     HEQNIHNLPRDQGPGNTVSLEVESENITERFFVVGEKRVSAEVVAAQLVKEVKRYLAS
                     PAAVGEYLADQLVLPMALAGAGEFTVAHPSCHLLTNIAVVERFLPVRFSLVEADGVTR
                     VSIE"
     gene            complement(53117..54343)
                     /gene="rtcB"
                     /locus_tag="ACHKBL_000249"
     CDS             complement(53117..54343)
                     /gene="rtcB"
                     /locus_tag="ACHKBL_000249"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-splicing ligase RtcB"
                     /protein_id="HFO5817175.1"
                     /translation="MNYELLTTENAPVKMWTKGVPVEADARQQLINTAKMPFIFKHIA
                     VMPDVHLGKGSTIGSVIPTKGAIIPAAVGVDIGCGMNALRTALTAADLPENLAELRQA
                     IETAVPHGRTTGRCKRDKGAWENPPVNVDAKWAELEAGYQWLTQKYPRFLNTNNYKHL
                     GTLGTGNHFIEICLDESDQVWIMLHSGSRGIGNAIGTYFIDLAQKEMQETLETLPSRD
                     LAYFMEGTEYFDDYLKAVAWAQLFASLNRDAMMENVVTALQSITQKTVRQPQTLAMEE
                     INCHHNYVQKEQHFGEEIYVTRKGAVSARAGQYGIIPGSMGAKSFIVRGLGNEESFCS
                     CSHGAGRVMSRTKAKKMFSVEDQIRATAHVECRKDAEVIDEIPMAYKDIDAVMAAQSD
                     LVEVIYTLRQVVCVKG"
     gene            54532..56130
                     /gene="rtcR"
                     /locus_tag="ACHKBL_000250"
     CDS             54532..56130
                     /gene="rtcR"
                     /locus_tag="ACHKBL_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RtcR"
                     /protein_id="HFO5817176.1"
                     /translation="MRKTVAFGFVGTVLDYAGRGSQRWSKWRPSLCLCQQESLVINRL
                     ELLHDARSRSLFETLKCDIASVSPETEVVSVEIELHNPWDFEEVYACLHDFARGYAFQ
                     PDKEDYLIHITTGTHVAQICWFLLAEARYLPARLIQSSPPRKKEQPRGAGEVTIIDLD
                     LSRYNAIASRFAEERQQTLDFLKSGIATRNPHFNRMIEQIEKVAIKSRAPILLNGPTG
                     AGKSFLARRIFELKQARHQFSGAFVEMNCATLRGDTAMSTLFGHVKGAFTGARESREG
                     LLRSANGGMLFLDEIGELGADEQAMLLKAIEEKTFYPFGSDRQVSSDFQLIAGTVRDL
                     RQLVAEGKFREDLYARINLWTFTLPGLSQRQEDIEPNLDYEVERHASLTGDSVRFNTE
                     ARRAWLAFATSPQATWRGNFRELSASVTRMATFATSGRITREVVEDEINRLRYNWQES
                     RPSAITALLGAEAENIDLFDRMQLEHVIAICRQAKSLSAAGRQLFDVSRQGKASVNDA
                     DRLRKYLARFGLTWEAVQDQHSSS"
     gene            complement(56112..56870)
                     /locus_tag="ACHKBL_000251"
     CDS             complement(56112..56870)
                     /locus_tag="ACHKBL_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HFO5817177.1"
                     /translation="MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELA
                     EQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTT
                     PEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
                     SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVN
                     MGSISMVDAVYTDAPPPVSVMQVLTDHHIQLELC"
     gene            complement(56887..57717)
                     /gene="glpG"
                     /locus_tag="ACHKBL_000252"
     CDS             complement(56887..57717)
                     /gene="glpG"
                     /locus_tag="ACHKBL_000252"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease
                     GlpG"
                     /protein_id="HFO5817178.1"
                     /translation="MLMITSFANPRVAQAFVDYMATQGVILTIQQHNQSDVWLADESQ
                     AERVRAELARFLENPADPRYLAASWQAGHTGSGLHYRRYPFFAALRERAGPVTWVMMI
                     ACVVVFIAMQILGDQEVMLWLAWPFDPTLKFEFWRYFTHALMHFSLMHILFNLLWWWY
                     LGGAVEKRLGSGKLIVITLISALLSGYVQQKFSGPWFGGLSGVVYALMGYVWLRGERD
                     PQSGIYLQRGLIIFALIWIVAGWFDLFGMSMANGAHIAGLAVGLAMAFVDSLNARKRK
                     "
     gene            complement(57762..58088)
                     /gene="glpE"
                     /locus_tag="ACHKBL_000253"
     CDS             complement(57762..58088)
                     /gene="glpE"
                     /locus_tag="ACHKBL_000253"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate sulfurtransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HFO5817179.1"
                     /translation="MEQFECINVAVAHQKLQEKEAVLVDIRDPQSFAMGHAVQAFHLT
                     NDTLGAFMRDNDFDTPVMVMCYHGNSSKGAAQYLLQQGYDVVYSIDGGFEAWRRQFPA
                     EVAYGA"
     gene            58278..59783
                     /gene="glpD"
                     /locus_tag="ACHKBL_000254"
     CDS             58278..59783
                     /gene="glpD"
                     /locus_tag="ACHKBL_000254"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HFO5817180.1"
                     /translation="METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSA
                     SSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIR
                     IGLFMYDHLGKRTSLPGSTGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANAQMVV
                     RKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYSWQARGLVNATGPWVKQFFDDGM
                     HLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYK
                     GDPKAVKIEESEINYLLKVYNTHFKKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDY
                     TLDIHDENGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIE
                     GDRDDYAARLRRRYPFLTESLARHYARTYGSNSELLLGNAGTVSDLGEDFGHEFYEAE
                     LKYLVDHEWVRRADDALWRRTKQGMWLNAEQQSRVSQWLVEYTQQKLSLAS"
     gene            complement(59837..60442)
                     /locus_tag="ACHKBL_000255"
     CDS             complement(59837..60442)
                     /locus_tag="ACHKBL_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000589351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5817181.1"
                     /translation="MIKFRLYIPPVILGFVIVPLLVWPTVIALAVLIFTLTFLAEIIF
                     SFPLLVVRISLQELQLELMVEYALFFSVMGGIGWQFSRRTPPELKNRLHYWLVFSPAY
                     FWLILSNFILYITPEKSALLENIRNFFLTFVWLPLNFSPFWPQPWTDFVGPISAQLGF
                     ALGYYCQWRSKNRSQGQKWVEWGTCLSLAILAQGPLFNYLQ"
     gene            complement(60445..61221)
                     /locus_tag="ACHKBL_000256"
     CDS             complement(60445..61221)
                     /locus_tag="ACHKBL_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5817182.1"
                     /translation="MQPSSAMQALIEQVYHIFRRYPVPQQFVVCCEYCLSQQEQKALR
                     NTSLRAIPYSLINAWNSSPGPDPQNSDEVRYFLPRLLEFVAQGQFDNIHEVFSLRRIN
                     LASKENWREDERAILQRFACQYMADWVSGDEAVELQYMLEMFSRADIALSPLLDAIIS
                     VPGYQPTVSMACLLWHNREENIQNSRFEQDDDDKAITAQINAWAFNNQFILKERARQA
                     IENLLNQPEQGTHYQVWEDDWMIDECLCAMYDASSESPGK"
     gene            complement(61224..61934)
                     /locus_tag="ACHKBL_000257"
     CDS             complement(61224..61934)
                     /locus_tag="ACHKBL_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5817183.1"
                     /translation="MELVDTLFASLSGTDPFTGVDITIANCKSTYWDEGIIQQLINQV
                     LDEGEKFAGTVGLEGLSRYDVTLNIGLTSSNVWPGFSLDTATIGRLCACGADFGFDPY
                     ISDVPDVQCDLNTTNDFTVHFTAMLNPDERVIIAKRPLKKCDSWIEDVYIFQVFKEAW
                     KFHTDNSLRGFRDKQAELKLYARHYSVENCTEESCRDCNYCIRPSFSLSRSSIIRLNA
                     ANARFVYQPFTRDQRKRG"
     gene            complement(62108..>62791)
                     /locus_tag="ACHKBL_000258"
     CDS             complement(62108..>62791)
                     /locus_tag="ACHKBL_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001588797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO5817184.1"
                     /translation="NYSTNYPADRSKADYWSSGTEGYIKIRLDKKIISDVSLNNVLLV
                     SLYASQRQTEHGPVPLFNAWISNLDIQVPQGCTINEGTSFTVNMPDVWASELSRAGAG
                     AKPAGVTPVATTIPINCTNKDTDAVMTLVFDGNISATRDTNGKQSIIQAQDNPDVGIM
                     IMDSQQNSVDLNALATSVGVPFRLVENQAASAQTADVTFLALPVSTTGKVPAAGRYNA
                     LAVLRVEYQ"
BASE COUNT        15227 a        17091 c        15659 g        14814 t
ORIGIN      
        1 aaatttatta aagttgcact ggtaggtgcg gtactggcta cgttaactgc atgtactggt
       61 catattgaaa accgtgataa gaactgctct tacgactacc tgctgcaccc ggcaatttct
      121 atttctaaaa tcattggcgg ttgcggtcct actgcacagt aagtctctga ctgaaagcgg
      181 ctaacaccgg tttaattgcc tgacgccctc ggggttaccc gagggcgtat ttatttctgc
      241 attcttttcg aaaacatcat gcacaagatt aaatactggc aatactctca cgcaaaatcc
      301 gcagcaccgt ctcttcaccg ctgcgatttg ggttaatacg aatcgcgtaa tgttcgagtt
      361 gcggattcgc ctggcgaaac gttccggaaa gtcgataaaa aagtggcgga atttcatatt
      421 tcgactctgc accaaccggg taaggcaagg caccgcgctt ttgcgcctct tccagtactc
      481 tggcggcaat cggctgatga aactccacaa tcaacacctt cgactgcgca ttggcaatca
      541 ccgcgctttt cacttccgcc actgcgccac cgttaagtaa agccagcaac cgttcagata
      601 cccccgcctg caccgcgtgc atcactggcg cgagcaccag accacgcaac acctctaatg
      661 cctgcgcccc ctgaatctga ctgccgccgg aatagagcgt ggcgcgaatc cggctaataa
      721 catcggcatc gcctaccacc gcaccaacgc cctctggccc aaatagcttg aagcaggaaa
      781 atgtcgacac attcgccccg cattcgcagc caattcgcga caccttcatc accgcatagt
      841 tgtcatcggt taacgctgga acacctgccg cgcgcaacgt tgccagcacg tctgccagca
      901 catagcggtc ctgcggctgc tggcgcgtat gctgcaccag cgcggcatca ggttgttgct
      961 cgtcaacaac ctgcttcagt gccgacagat cattgaaatc agccgtaatg agcgttagcc
     1021 ccatctgctc aataatgacg cgcgtcgttg gataaaccgg cgcatcatgc accagaagac
     1081 gttgccccgg tttgagcaaa gccgccagtg ccgcacgaat cgcgccagta cccgcgccct
     1141 gtaccagtgc cgccgcgggt gcatgaaacg cgtcagccag cacctgttcc acacgctggg
     1201 taatgcgggg ctgatttagc cccggcgtta agcccaaatc accgcaggta agaaactcgc
     1261 tgccaggaaa atgacggcaa atcgtgtcca ccagcgcaaa ctgcttttgc tgcgcctcaa
     1321 taagcgtcag gctttgcaga ggaaacgtct tcataggtgc cttccttaag ccggaacgaa
     1381 caggccaagc cagtaaagaa cattcagcag gatgccggtg atcattactg ctacgaccgg
     1441 tgccgccatt ttctgtaccg gacggcccag ggattcgttg aggaagtaaa tggcaaccgc
     1501 gatagagaat ccggtatagc ccgccatttt gattgctgca aagatcgaac cgaccagcaa
     1561 cgccacttcc atcagcatat tcatggcgtt acggatgtta tccgacgcat tacgtaccga
     1621 cgggtagcgc cccaaccatt tgccgatcga acgaagcagc aagacttccg ccgaaataac
     1681 cactgcgcct aataccgctg caaccatcgg attcggcgag agatagccca ccgcataaac
     1741 aaaggtaaag cccgcaactg catacacacc agtggctaac gcggtggtgg caatcaacgg
     1801 tacaaaaccc agcccgcgca taaattccgc cagagctgcc tggttgatta gtgtttgcga
     1861 ttgttccggc gttacgcctg cggaatatgc tttctccagt gtgaagatcg acacttcact
     1921 gccagcaaaa atcttcatgc tggcaacggc ggcaatcagc gccccgacaa tagcgatata
     1981 cggtaagttt ttgataatcc gtgacgtgcg ttcttcaaac accgaaagcc cgctggcatc
     2041 aatgtcattt tcatcacgat gacgcaggtc gtgagtaatc gcgatcccca gcagcatcac
     2101 catgccaata aagatttcga tggattccgg attgagatgc gggaaatagc gcactactac
     2161 cacacgggtc atcagtacca ccacggcggc gatcagactt tgcttccagc caaactggta
     2221 gaaaatcgcc accaacggga acagtgcaaa agctgaaacc accggcgagc ttaattcccc
     2281 caggctacct aatacatcca ccggtagcgc ggtcagcagc tggtttaccg gtaacaggca
     2341 agtaaggatc aacacgcccc agatagcgcc aagaccaaac gccatcaggc tgtttatcgc
     2401 cagtacgccg aggatatcgg taggaagaaa gagtaaccat gcgttgagca gtccggtttt
     2461 cagggtaaaa gagatcccca ccgaagccac aaaaccaatg ctcagaccaa aagcgatact
     2521 ccccgcctcg cgacggttca tatagccttc aatcagttgc ggcaggatcg ggcggatacc
     2581 gtcatgaaaa acggccgccg agcgatgcgc cagaagcgat gtcatacccg tcaggcacgc
     2641 caccacgata atctgaatat acagatccat agtcgtgcct tttatttcag atggttaatc
     2701 agcatcggaa tcgcgtgttc gacgtgctca acggaaagcc caaacgccac tttcccttcg
     2761 gcgatcattt tggcgatatg ttcgtctttc gctttaatgc ctggtttggc aatggtgcaa
     2821 cttttgttat agccaatcac cgcgatggca atcgacaatg ccgcgcccgc accggtatta
     2881 cacgcgccga tgtagtaatc cagttgcccg gatttgactt tcattgccgc ttccatgtcg
     2941 ttgtgaatga aaacttcaaa acagccagga gccgtcgctt caatggtttt tttaatctgc
     3001 tcacgctgta agcctgcaac accaatcttt ttcatgagtc tgtccttatt ggaaaaattg
     3061 agagggagtt tcacgtaaca tcacatccac atcggcctga ctgaatcctg actggcgcaa
     3121 ttgcggaata aaggtggtca ataaatagtc atagccataa ccaccgttgg cttttaaatg
     3181 ggagcggcgc gtaatatcca tcgacagcat gacgcggtta agcaaacctc ggtcacgtag
     3241 cgcgtggagc atcgcaatgc gcttttcgtc cgggtagtaa ctgttcttac cgatggtgtc
     3301 gaactgcaca tacgcgccga gatcgatcat cttcacaatg ttgtcgaggt tgtctttcag
     3361 atcgcagtga ccaacggtga cgcgcgaaag atcaactccg tgggcttgca gtagcgccag
     3421 ttgttccagc cccatggtgc taaaagaggt atgcgtggag atcgggcgtc cggtctggtt
     3481 atgcgccagc gcggcggcaa taaatacctt ctcttccagc ggcgtgattt ttccttcgct
     3541 ggagcctatc tccgcgatga tcccggcttt cagctccgtg ccatcgatac cctgttcaat
     3601 ttcatcgacc atctcttgcg ccagttcctg cacgctgcgg gtcgccacat gttccgggaa
     3661 aaacgcgtcc tggtaataac cggtacaggc caccacgttg atcccggttt cgcgcattac
     3721 atcgagcata aattgcgcat tgcgccccat gtaacggttg gtcatctcaa tcacattacg
     3781 cacgccctgg gccatcaggt cgttcatctc ctggcaaatg aacgcatact gatcaaggcg
     3841 gcagtccacg ttgtttttaa agccggagag atcaatatgc agatgctcat gggcgagggt
     3901 gtagcccgtc ggatcaaaac tcatagggtg tctcctgcaa accggaggga ggaaagaaaa
     3961 gagcgaccat tttgtggcgg tggcgcgcgg aaaaattcac atacggtagc ccccacatca
     4021 gaaagcgtgg tgagcacacc aagctgcgta gcgaccatcc cttgctgata aaccagcact
     4081 ggcaccactt cgcgggtatg gtggctgtga ccaatggtcg gatcgttacc gtgatccgcc
     4141 atcacgacca ggcaatcatc tggctgcatc gcctcaataa gccgggcaag gttacggtca
     4201 acgacctgca aacgttccgc ataacgtgcg acgtcttctg catgaccagc gaggtcggtt
     4261 tcctggatat tggtacaaat aaacgccgtc ggatgggtgt taaattcgtt gaaggtgata
     4321 tccataatcc gctggctatc caccaggttt tgccagctca cgccataagg attactggca
     4381 atatccgcca ctttccccac cagcacggtt ggtacgccca cttcatacag tttttgtggc
     4441 acctgcactt tttcatcgac gccgtagccc atatgcacga cctggaagcc gttgtcataa
     4501 gcgccagaac gcggcgcatt gatgccaata aagcgccctt ctttgctttc tgcggcatcg
     4561 aggatgcgct ggctgtcggt taacaggcca ccaaatgtaa tgacccgacc gacctgtacc
     4621 tgctcacgca cgatacgacc aattttgatt gcgtcgtcaa aagagatcac agagagattg
     4681 gcggtgatgt tatagacctg acctaaatcc gcctcgagat tatcgccaat cgcaaccgcc
     4741 tgattgaccc acagaaattg cagatcatcg ccacggcgct ccacctgcca gtcagcgtta
     4801 agtaatgctt gctcaacacg gtcaatcaca tcgcgaaagg gcatccgtag cggcggtaac
     4861 gggcgcgtgc ctaaaatttc ctgatgcccc ataaaggtat cgccaccttc atgttgcagc
     4921 tctgccacgc cccaggttgc ggagtctgac ggctgcatat ctccaggcgc ataacctaat
     4981 gcgttgatta gccccatttt ctccagcatt ggtagctgca aatgcggcaa ctggctcagg
     5041 atgtgaccac aggtatttgc cccggcatcc tgcggacgca ccaacgtgac atctttcatt
     5101 gcccctacgc caaagctatc aatcactaac accacaaatc gcgccattac gcccccagag
     5161 agtttccaag actgtcataa cgaccgacga tttctggttc gccacggtga ataccggaca
     5221 gcaacaccac atcgctacgg gtgacaaaaa tctgcgtgcg aaaacagagc actactgcac
     5281 tgcttaccgg aaactcgcct gccagcggca gggtgtagtc gatactgctg tcatccacag
     5341 ttttgagatt ggtttcagta atcttttgat tttctggcgt aaacaccagc gcatgttgcg
     5401 cgtgaccacg acggtagtaa ccgccgccgt agcagtagct gtcgccacga aaatgatggg
     5461 agatttcact taaccagagc atcgcgatac gttcaggctg atcgccctgc tggtttgccg
     5521 gaatcgtccc cgtcagcgca tgaccgggtt cggcatgagt cacaccgtat tccgccagca
     5581 atggcagcga agtgcagctg gtcgcagaag gtgcgttcag ttgctcaatc gcaataccag
     5641 atttcgccag ttgatcccgt gcctgtatca gcgtgtgaag attcggtgtc ggcaaaactt
     5701 tcccggcagc ctcatcccaa agcaggcaag ggaaatgggt tagtccggct aaatgcagcc
     5761 ccggcagttt cttaatatca gcaaccacct cgtgcagcga atgctgtaca aaaccgctct
     5821 cctgccccgg ataaagaaaa tcatcgtcgc tataaacttt gagcagcaca gactgcacac
     5881 gtccggcctt caccgctgct gcagaaactt cccgcgcttt atcgagagta aacacggtga
     5941 tgacgtcagt tccctgtgca acggcgtcag aaacctggtg acaagggatt tgcaccagat
     6001 gcccctgatg cgccaacggc agaccggcgc ggcgcatgac ccgcgcctct ttgtaatcca
     6061 ccgccacaat gccgctgtag cccagtgcca gaagtttttc cgccagccac ggattacggc
     6121 caaactgctt ggtcatcaga tacagttcaa tgccgtaaag tcgcgccgtc tcaatcagcc
     6181 gcttaccgtt ttccagtatc tgatccacat cgatcaccca gctgtccggg gcgatcttgc
     6241 cctgctgcca caaacttagt gcggcggaaa tcagcgccgg gttctggcgt ttcaatgctt
     6301 ctacaaacat cttgttgcct cgtttctgtt ccttgaatta aatattcatt tttctgaata
     6361 tttagattat gcgacgtgga tttgtcggga gcttcaaacc tcttcgttag cagccatcca
     6421 taatccataa agattagcca gcaaatagcc ttcttcacac ggcttaactt ccagtgcgaa
     6481 ttccttcaac aacacctgat gcaattgcac aacggcttgc cagtggctgg attgcgccag
     6541 ctctgccagc aattcgtcat ccagcggctc tatttcttca ccacggcgac tgcgcatcag
     6601 tgcgctcgcc atatgtgtca tcgccatcat tccttgctcg ctgcgcaccg gtagatgaca
     6661 ctctgtttcc agtacgttga cgacctgcat catgcctttg cagatgtcct tatcaataac
     6721 gcctgcctca caaagcaggt tgagtctggt ttccatttca gatgcctttc gtttaataac
     6781 tcggttcctg ttccccactg accactcgtt gttgatgggc aagcagggcc tgtttatcaa
     6841 caactgcacg cagcaattgt tgcattgggt aatcatcgac tcgcgtcaaa actacggctt
     6901 cggtggcctg taaaaatcgc ggatcgtctg tgagcggcgt cgcttctaat cccagcatgg
     6961 tcagttcgtt ttccgccacc acgttccaga tcaccgcatc aacatcgcct ttaacaatgc
     7021 gttgtaaact ctcgtgataa gagagatcga ctcgttccac atcactatcg ccaaaaaaga
     7081 catcggtcat gattttctga tccgccgaac ggttatccag ccccacgcga ttcacgtttg
     7141 cggactcgcc tttacggcaa atcaactggt gctcgccaac gtaggtgtgc ggccccaact
     7201 ccagcgcgag gcataaccct ttttgcgtga gataactttc cgccgccagt cgggaaacaa
     7261 ccgccatgtc atacacgccg ttaagtaaac actccacgcg aatatccgcg ccgcgcatgt
     7321 gcgcatagta aaaaggaata ccatcaaact gagctttcaa tccgctcgcc aggccttcgt
     7381 acaaacgggt atagggcaag ggcatcgcgc acaccacatt gttgatatcc acatgactca
     7441 gcaatgcttt gttatccatt tccaccagat aactgccatt acgcccacgg cgttctatcc
     7501 gtatcgctcc gcttgattcc agcgttttca acgcggcctg cgtcaggcca acggatgaac
     7561 ggcattcagt tgccagttca tctatggttt tcagtcgatt accgcacttt tcacccaaca
     7621 aataacgggc cagtgtcgtg atgacgacgc cttctttttt gataaacgtt cgacgcataa
     7681 taaattttca gtaaattgaa tatttatatc ttcaggaatt tgaagataag gcgcaacagc
     7741 gagatgtgga aacggcgagg cacttcacat tttttcggat tattgcaaat gaggataaaa
     7801 aaaccgggtt tccccggttt cagagtgatg ataaaagcaa aattgcctga tgcgctacgc
     7861 ttatcaggcc tacatttcct tgcaatatgt gcattacttt gtaggccgga taaggcgttc
     7921 acgccgcatc cggcatgaac aaagcgcaat ttgccagcaa tagtgaatta cggcttcgcc
     7981 acaaaaccaa tcgcttcgta caccgctttc agcgtacgag aagcgtgtgc actggctttt
     8041 tccgcgccat ctttcatcac ctgttgcagg aaggcttcgt cattgcggaa acggtgatag
     8101 cgttcctgca attcagtcag cataccggaa acggcatcag ccacttcgcc tttcagatga
     8161 ccatacatct tgccttcgaa ctgtttttcc agttccggga tgctctggcc cgttaccgca
     8221 gaaaggatat ccagcaggtt ggaaacgccc gctttgttct gcacatcgta gcgaactacc
     8281 ggcggctcgt cggagtcagt gaccgcacgt ttgattttct tcactaccga tttcggatct
     8341 tccagcaggc cgataacgtt attgcggtta tcgtcagact tggacatctt tttggtcggc
     8401 tccagcagcg acattacgcg cgcgccagat ttcggaataa acggctccgg cactttaaag
     8461 atctcgccat acagcgcgtt gaaacgctgg gcgatatcgc ggctcagttc caggtgctgt
     8521 ttctggtctt caccgaccgg taccagatta gtttgataca gcaggatgtc cgcagccatc
     8581 agcaccggat agtcaaacag accagcattg atgttctcgg cataacgcgc agatttatct
     8641 ttaaactgcg tcatgcggct cagttcgccg aagtaggtat agcagttcag tgcccagcct
     8701 aactgtgcat gttccggcac gtgggactga acaaaaatgg tgcttttctc aggatcgata
     8761 ccacaagcca gatacaaggc cagcgtatcc agcgtcgctt tacgcagctt ctgtgcatcc
     8821 tggcgcacgg tgatcgcgtg ttggtcaacg atacagtaaa tgcaatgata gtcatcctgc
     8881 atgtttaccc actgacgcag cgcacccatg tagttaccaa tggtcaattc acctgagggc
     8941 tgtgcgccac taaaaacgat gggcttagtc atttttcgat tcctgatttt cgctatgcgg
     9001 aagccctaat gcgggcagaa ggtcatttat agactgataa attacatcag gctggctgag
     9061 atcgatagcc tcgccgtagt tatatccgta ggttaagcca actgatgggc aacctgccgc
     9121 ttttgctgcc tgaatatcat tgcgtgagtc gccaacaaac agcatctgtt gtggggcaat
     9181 tcccatccgc tcagccacca gtaacagcgg gtccggatgc ggttttttgt tttgcacatc
     9241 atcaccgccg ataaccacgc tgaagtattt ggcgatatct aaggcttcga gcagcggcgc
     9301 gacgaacggc gtcggtttgt tggtgaccag gcctagcggc aggcctttag cctgcaatgc
     9361 gcccagcgta tcggcaacgt gcgggaacaa aaacgtcccc tcttcggcaa cctcgccata
     9421 gtagcgatcg aacagtttac gcagaatacg tacctgttct tctgccggaa tgtcgtcatc
     9481 aacgggcggt ttacccattg ttttacgcag agtcgcacgt tcctgacgcg cccaggtcaa
     9541 tgcgcgctcc atcagaatat ctgcgccgtt accaatccag gtaataacgc gttcttcacc
     9601 tgcgacaggc aactccagcg catacagcgc catatctacc gcagcagcaa gaccgggtgc
     9661 actgtcaacc agcgtaccgt cgagatcaaa agcgacgccg cgaatatctt caaacttatt
     9721 catgacttac ctttgccagt tcactgcgca tttcatcaat gacttttttg tagtctggct
     9781 ggtcgaagat tgccgaacca gcaacaaaca tatccgcgcc cgccgcagcg atttcgccaa
     9841 tgttgttcac cttcacgcca ccgtccactt ccaatcgaat gtcaaagcca gactcgtcga
     9901 tacgacgacg tacttcgcgc agtttatcca gtgtttgagg aatgaaagac tgaccgccga
     9961 aaccaggatt gacggacatc agcaggatca catccagctt atccatcacg taatccagat
    10021 aactcagagg tgtcgccggg ttaaatacca gacccgcttt acagccattt tctttaatca
    10081 gttgcagcgt gcggtcaaca tgctcggagg cttctggatg aaaggtaatg atgctggcac
    10141 cagcggcagc gaaatcaggc acaatgcgat cgacgggttt caccatcagg tgtacgtcga
    10201 taggggcggt aatgccgtag ttacgcaagg atttcagcac cattggccca atagtcagat
    10261 tgggaacata gtggttatcc atgacgtcaa aatgcacgac atcagcgcca gctgccaggg
    10321 cttttgcggt atcttcaccc aggcgggcaa aatcagccga cagaattgag ggggcaatca
    10381 aatactgttt catccgcttc tccttgagaa ttattttttc gcgggtgaaa cgactcctgg
    10441 tttgtacaaa gccagcagtt cgtccacctt tttacgtgtg ccgccgttgc tgcttatact
    10501 gcgtcgaact ttgacgacat gcaattttgc gcgctgatac cactcacgcg ttaacatcgt
    10561 atcgtgattg gagatcagca ctggaatatg gcgatcaacc agaccttcgg cgatctccgc
    10621 cagatgcgct tgttgttcaa gcgtaaaatt gtttgtgtga tacgccgtaa agttggcggt
    10681 cgcagacagc ggcgcataag gcggatcgca atagacgacg gatgcatcat ctgcgcgcgc
    10741 catgctatcg gcgtaagact cacagtagaa aaaggcattc tgcgcttttt cagcgaagtg
    10801 atacaactct gcttccggga aatagggttt tttgtagcgg ccaaacggca cgttaaactc
    10861 accgcgcaga ttgtaacgac acagaccgtt gtaaccgtag cggttcaaat ataaaaacag
    10921 taccgcccga cggaacggat cctggctttt gttgaactct tcgcggaact gatagtaaac
    10981 ctcggcgcaa tttgtttcgg gaacaaacag ctcgcgtgcg gcctgtacgt actcatcagt
    11041 acgcatcttc acaatgttat agagactgat caggtcgcta ttgatatcgg caaggatata
    11101 acgagaaaag tcggtgttga gaaacaccga cccggcacct acaaaaggct caaccagaca
    11161 ttcgcccttg ggcaaatgcc gtttaatatc atcaagcagg ggatacttgc cccctgccca
    11221 cttcaaaaaa gcgcgatttt tcttcatgct gactaactaa ttacaccttc tccggctgtg
    11281 gagaaagctc cgacagcatc cagcgcttca ggcaactccc gcgatgataa accattgcgg
    11341 gagataacct tgattacttc agatcggcct gtacctgacg cagcggtttc gcccacgggt
    11401 ttttggcctg gacatcggct ggcaatgtag ataccgcttt tttcgcctct tctttcgaag
    11461 cgtacacgcc agaaaccagg acataccacg gctgaccatt acgcgtcgtt tcatagacaa
    11521 cgtagttttt cagattctct ttcttcgccc aaccgttcag gttgtcgtag ttagaggaac
    11581 tgctcagctg cagagtgtaa tggctggacg gtgccgattt caacgaacca acattacctg
    11641 cggtcttccc tccagcggct ggtgttgccg tggtttgcgc cggggatgcc gtctgtactg
    11701 gagccgtagt cgccgtctct tttggtgctg gcgtagaagt cgcagccggt gccttcgtgc
    11761 ttgccacagg agcagctggt tcagtacgct tcggcgtctg tgctaccggc ttcggctccg
    11821 ttttcacggt tgcttgcggt tttttcggtt caatcaccgc ctgctggcga gctggacgcg
    11881 tagtggccgg acgttcagcg gtttgcgttt tcgccgtgtc acgcgatgca ttgccattgc
    11941 gaacaggcgc gaccgtcgcg ggttcagtcg gcaacgtgga attgaccgcc acattattca
    12001 actgttgctg attttgtggc tgggtcagcg cattgttcag gtcaccctgc acttcaacac
    12061 gttgttgacc atccgttgcc gccggggttt gcccttgagt cggcgtagaa gagatcggcg
    12121 gcagagaaac atcctgctga gtattttccg cagacgtggt tcccggcgct ggctgcacgc
    12181 cattcgcctg atcggtcgca ttgccagcaa gatcaatact cttctcgcca gacgcggttt
    12241 gatcgctgga agaggtcgag ggggctttta gcgcagaacc gataccgatg atcaacagca
    12301 ggagaaccag aatgccgacg cccatcatca tatactgacg agaagcgggt ttactggctg
    12361 cttttttgcg cttacgtgga cgacgctcta cgcgctcttc atccacggtt tcatcttcgg
    12421 attcgtcaat ttcttcttcg agttccggtt cctcattgcg ctctttttgc gcacgagtcg
    12481 gacggcgatc gtcagtgtca tcaagttcaa tatcatcaaa attgatctgc ggttcgccac
    12541 gttcagtacg ctcagaacgt tcagaagatt gacgagaacg accagtacga cgatcgctgg
    12601 gatcgggttt cagctcgtct tctggtttga attcatccat ttaacacccc actaaaaggt
    12661 taatgcttac cacgttgcaa ttaacctgaa gctaatagac cgcttgataa gcggcctgac
    12721 ctttcttgtt gttacgctga ttgacaatcg gcaatggcgt taagaacaag ctcgtgcgaa
    12781 acgccgctgc gaacttcact cttaccaatt gccaacggaa gaattaagcg catctctccc
    12841 gcaaggactt tcttgtcacg cagcatatgc ggtaaatacg cctgcgcaga catttcgcgc
    12901 ggcccattga ccggtaaccc agcccgcgtg agcagggtta taatacgctg cgtttcggca
    12961 gaactaaact gcccgagacg ttccgacgtc cgcgccgcca tcaccatacc cgcagcaacc
    13021 gcttcaccgt gtaaccaatt gccatacccc atttcagctt caatggcatg accaaaggtg
    13081 tgtcccagat tcagtaaagc acgtaacccg gtttcgcgct cgtcggcggc gacaacttct
    13141 gccttcagtt cacaacaacg gcgaatacag tacgccattg ccggaccgtc cagacgcaac
    13201 aacgcatcca gattctcttc cagccagttg aaaaacgcac cgtcaagaat aatgccgtat
    13261 ttgatgactt ctgccagccc cgacgctaac tcacgcgggg gaagcgtttt cagacagtcg
    13321 agatccacca ccactgaagc aggctggtag aacgcgccaa tcatgttttt accgagggga
    13381 tggttgaccg cagttttgcc gccaacggag gaatcgacct gcgacagcaa tgtcgtcggg
    13441 acttgaatga aacgtacacc gcgctgataa ctcgccgccg caaagccggt cagatcgccc
    13501 actacgccgc cgccaagcgc caccagcgta gtatcgcgac catgcggctt ttgtaacaac
    13561 gccgtaaaga cggtatcgag tacagccagg cttttatact gctcgccgtc agggaggata
    13621 acgctatcga cgttaacacc cgcctgttca agtacgccgc ggaccttatc gagatacagg
    13681 ggagccaggg tttcgttggt aaccaacatg acctgctcgc ccgatttcag cggtaagaat
    13741 gaagctggtt cattaaacaa accagatgcg atggtaattg ggtaactacg ttccccgaga
    13801 gtaacgacaa tcctctccat aacgcgacat ccaccttaat tactgtaccc gcagacgagt
    13861 gtatataaag ccagaattag ttgctttcca gcatgtgaat aatctggttt gcaaccactt
    13921 tagcgctttg atcatcagta cgaatggtca cgtcggcaat ctcttcatac agcggattgc
    13981 gttcattggc caacgcttcc agaacttcac gcggcggtgt ttcaacgtgc agcaacgggc
    14041 gttttttatc acgctgcgtg cgtgcaagtt gcttttcgat ggtcgtttca agataaacga
    14101 caacgccacg agcggaaaga cggttacgcg tttcacggga tttcacagag ccgccgccag
    14161 tagccagcac aataccctgt ttctcggtca actcattgat gaccttttct tcgcgatcgc
    14221 ggaagccttc ttcgccttct aaatcgaaaa cccagcccac atcagctccg gttcgtttct
    14281 caatctcttg atcggaatcg taaaattcca tattgagttg ttgagctaac tggcgcccaa
    14341 tagtgctttt tccggcaccc ataggcccaa ccagaaagat attgcgtttc tctgccattt
    14401 tttcggtact actaagacta ttcgttaatg ataaacccgc ttcgctcaga gagcgccgca
    14461 ggacatgaac tgaaacctca taagatattg cgagagtcag actgaaaatt atctcaatac
    14521 tccagcgggt ttggcaactg aataaatcac ccatccctct gcatatctgg tcgctgcaag
    14581 cgcgctgcct tgctaccacc gctctggcga ttaatcaccg ggtaagatta gcgtaaaaaa
    14641 gacagcaaaa tgccgcctga atgataaatc atcatcatgg aacgcaggca gcgaagagca
    14701 ccaactctca aatcggtact ccttgtatgc taaatacctg cgcgcgtcaa atagatgaaa
    14761 cacgttcagc gtaaaaacat taccgctttt acggctgttt actcactgga aaccagtcgt
    14821 ggcgtgataa acaccactaa ctcgcgtcgt tcatcttctt ttccgtcatg acgaaataat
    14881 tgcccgaacc agggaatgtc gccaagcaac ggtacgctat cctgacccga tttattttta
    14941 cgggtaaaaa tgccgcccag cgccaacgtt tctccgcttt tgacctcgac ctgcgtttcg
    15001 atctcctgct tatcaatcgc caacacttcg ccatcggcct gctgtagcac ctgccccgga
    15061 acgttctggc tgatgtgtaa tttcagccgg atgcgacctt tttgtaacac cgtgggcgta
    15121 acttccatcc ccaggacggc ctctttaaat tccaccgacg tcgcgccact ttccccgctg
    15181 gaaacctgat atggaatttc gctcccctgt ttaatgctgg caggctgaag atgtgaggcc
    15241 agcagacgcg gactggcgat aatatccagc tgctgttttt gttcgagcgc ggaaagctca
    15301 agatccagta aacgtccgtt gatgcgtcca atgttaaaac cgacatgcgt tgtcgccgtc
    15361 gctacggaga ggtcgctgcc aagcgtggtg acttgcccaa cgccaccagc ttgctgcgca
    15421 tcagccaacg tccatttcac gcctaactca cgcaaacttt tttcattaat ggtgacaata
    15481 tgcgccgaca gctcaacctg cccgaccggc agatccattt gcgctaccca ctgttcaagc
    15541 gcgcttaacg ccgttttgtt atctcgcagc aaaaggcgat tggtgcgttt atcgacggtc
    15601 atactccctt tggcactcag tagcttctcc ccggctttcg ccagttctcc cgcgtcggcg
    15661 tattgcaggg ttatactgcg attttccagc ggcagatttg cctgcgcccg cgcctgctcc
    15721 gcctcctgac gggcgatatt gtcattctgc caggcaacgg aatgcaccga gagaatgttg
    15781 ccctcctggc gcgttatcaa tccggcgctt ttcactacag tttgtagtgc ctgcttccag
    15841 ggaacgtcag ttaaatgtaa cgacaccgta ccgctgacgt ctggcgaaac caccaggttc
    15901 aacttctcct gttcagccag cgcctgcaac acctgagcta ccggaacgtc atccaccatc
    15961 agcgtcactt tttgcggctt tgccgcctgt acgccgggta taagcatcaa cagtagtgcg
    16021 gctatccatt gcttcatttg tatctccttg ccgttgccac aaccagtgtg gcggttcgca
    16081 gtttgtcccg gttcccagcg ttagtgcgtc tggcgtcagc tgtaaaattg tccagccgtt
    16141 ttccagcacg tcgttttgct gcacccgtcg ccattttttt tgcccgtctt ttattacacc
    16201 gatgatgcgc tcacctcgcc ctaccatccc ctgatagcgc cactggctaa gttcgctaat
    16261 ccggcataga tcttccggcg gtttaaaagg gtcacgcata ccggttaaaa ggcacaatgt
    16321 aatacctgcc aacaaccagc gtttaaccct catgcggcgt ctccagttgt agcgtgaaca
    16381 aaaggtcatc accttccacg cttaacgaaa aacggctcac gctgacgttg cgctctgcca
    16441 gccgtgtaaa tgccgacggc actgcttccc acagcgtttt caacgccaac tcacctccct
    16501 gcgcggatgg atgccagtaa accagttgcg cgccggataa ctgaaaatcc agtggcgaaa
    16561 agggcagcgt tttttcctcg ctgaagggag tggtgtcaac caggcgatac agtgcggccc
    16621 actgctggtg atgacttgcc cgtagtcgaa ttaatgcgtc gctctcttca tggtgtgtcg
    16681 atgacagaaa aatgagcgta actaacatca gcaaccagac tgcccagcaa agctggcgga
    16741 ggcggggtga tgtggcgaac caccagtcaa agagcatgtc catcgctaac cttccttgtt
    16801 aactgatact caaattgcca gcgtccctgc gcatcctgct gcgtggcccc ccgctgattg
    16861 agatgaaaag atgcatcctg gcggagtgac gtttccagcg cgtttaacgc ggtaatgctt
    16921 gttgtcagcc ccttgatctc cagcgtacct tgctgccagc ttatcgttgt cagccaggca
    16981 tgttcgggta aaagcgccgc cagtgcttcc agcgcagatt gccagtcacg ggtaaattgt
    17041 cgctggagct gcctttgcaa acgctgttcg cgtaactgct ggcgttccag caaacgtggt
    17101 ttcgttatct gtaagctgcg ggcgagttgt tgttccgcct gaagcaaaac ggtgtctacg
    17161 cgtgcttcgg cgttgcttgt cagacgcagt attagcgtta tcccgacagc cagcagcaga
    17221 ggcgcaacga acatcagcaa ccagaaacgc agaaaagcgg tccggcgttg ctgtcgccag
    17281 ggcaaaaaat taattggcgg gttcatcagt acgccttccc tagcgccagc cccagcgcga
    17341 tggcaaagtc tccacctggc ggcggtagcg gcggctgacg aacagaaacc gcctcccagg
    17401 gatcaaatcc gccttcgcca catatcgcga cgctttcggg atcaacggat aacgctgccg
    17461 ccagctcctt cgcgctagtc atccccaccg ccagtttgcg cccccagcga tagcgcgtcg
    17521 cccacagcca ctgttcgtta tcacgccagg ccagacattg ctgatgagaa ggtaaaaaag
    17581 gcaggaatcg ctgtaatgca ctggcatccg gggtgatcgc gctaacatga acacgcaacc
    17641 tttctgccag agtaagcagc gttgccaact ctttgctttg cgcggcagtc acgttatagg
    17701 cgggactgag tgagtcttcg ctgtaatcga agcgcaggga gtccggatcc atatccagct
    17761 cgcgggccat cgtcccggac agccaggccg tttgctcccg ctcaccaagg gacatcgacg
    17821 gacgtggaaa tgaccgctgt aatgtgcgac tggcgggaaa cgccaacata atgtgatgac
    17881 gctgcggcag ttcgcgactc cacggtaaca acgttttagc cagccgctgc acatcaacaa
    17941 tccgcccatc tttgataatg tcgttctcca gcggcaaccg ccaccagcgt tgcaaaaagc
    18001 attcttttgt gccccaaccg ctaccgcttc ttgctgttgt aaatgcaaac caatttgcca
    18061 gatcttaaat gccattgtga tgatctcctt atcacccgtc actctgacgg gtatatcaat
    18121 gcgtctggct tgcctttata ctaccgcgcg tttgtttata aactgcccaa atgaaactaa
    18181 atgggaaatt tccagtgaag ttcgtaaagt attttttgat ccttgcagtc tgttgcattc
    18241 tgctgggagc aggctcgatt tatggcctat accgctacat cgagccacaa ctgccggatg
    18301 tggcgacatt aaaagatgtt cgcctgcaaa ttccgatgca gatttacagc gccgatggcg
    18361 agctgattgc tcaatacggt gagaaacgtc gtattccggt tacgttggat caaatcccgc
    18421 cggagatggt gaaagccttt atcgcgacgg aagacagccg cttctacgag catcacggcg
    18481 ttgacccggt ggggatcttc cgtgcggcaa gtgtggcgct gttctccggc cacgcgtcac
    18541 aaggggcgag taccattacc cagcagctgg cgagaaactt cttcctcagt ccagaacgca
    18601 cgctgatgcg taagattaag gaagtcttcc tcgcgattcg cattgaacag ttgctgacga
    18661 aagacgagat cctcgagctt tatctgaaca agatttacct cggttaccgc gcctatggtg
    18721 tcggtgctgc ggcacaagtc tatttcggta aaacggtcga ccaactgacg ctgaacgaaa
    18781 tggcggtgat agccgggctg ccgaaagcgc cttccacctt taatccgctc tactcaatgg
    18841 atcgtgccgt cgcgcggcgt aacgtcgtac tgtcgcggat gctggatgaa gggtatatca
    18901 cccaacaaca gttcgatcag acacgcactg aggcgattaa cgctaactat cacgcgccgg
    18961 agattgcttt ctctgcgccg tacctgagcg aaatggtgcg ccaggagatg tataaccgtt
    19021 atggcgaaag tgcctatgaa gacggttatc gcatttacac caccatcacc cgcaaagtgc
    19081 agcaggccgc acagcaggcg gtacgtaata acgtgctgga ctacgacatg cgccacggct
    19141 atcgcggccc ggcaaatgtg ctgtggaaag tgggcgagtc ggcgtgggat aacaacaaga
    19201 ttaccgatac gctgaaggcg ctgccgacct atggtccgct gctgcctgcc gcagtcacca
    19261 gcgccaatcc tcaggaagcg acggcgatgc tggcggacgg gtcgaccgtc gcattgagta
    19321 tggaaggcgt tcgctgggcg cgtccttacc gttcggatac tcagcaggga ccgacgccgc
    19381 gtaaagtgac cgatgttctg caaacgggtc agcaaatctg ggttcgtcag gttggcgatg
    19441 catggtggct ggcgcaagtg ccggaagtga actcggcgct ggtgtcgatc aatccgcaaa
    19501 acggtgccgt tatggcgctg gtcggtggct ttgatttcaa tcagagcaag tttaaccgcg
    19561 ccacccaggc actgcgtcag gtaggttcca acatcaaacc gttcctctac accgcggcga
    19621 tggataaagg tctgacgctg gcaagtatgt tgaacgatgt gccaatttcc cgctgggatg
    19681 caggtgccgg ttctgactgg cagccgaaga actcaccacc gcagtatgcc ggtccaattc
    19741 gcttacgtca gggactgggc cagtcgaaaa acgtggtgat ggtacgcgca atgcgggcga
    19801 tgggcgtcga ctacgctgca gaatatctgc aacgcttcgg cttcccggcg caaaacattg
    19861 tccacaccga atcgctggcg ctgggttcag cgtcattcac cccaatgcag gtggcgcgcg
    19921 gctacgcggt catggcgaac ggcggcttcc tggtggaccc gtggtttatc agcaaaattg
    19981 aaaacgatca gggcggcgtg attttcgaag cgaaaccgaa agtagcctgc ccggaatgcg
    20041 atattccggt gatttacggt gatacgcaga aatcgaacgt gctggaaaat aacgatgttg
    20101 aagatgtcgc tatctcccgc gagcagcaga atgtttctgt accaatgccg cagctggagc
    20161 aggcaaatca ggcgttagtg gcgaagaccg gcgcgcagga atacgcaccg cacgtcatca
    20221 acactccgct ggcattcctg attaagagtg ccttgaacac caatattttt ggtgaacccg
    20281 gctggcaggg tactggctgg cgagcaggtc gtgatttaca acggcatgat ataggcggaa
    20341 aaacgggtac cactaacagt tcgaaagatg cgtggttctc gggttacggt ccgggcgttg
    20401 tgacctcggt ctggattggc tttgatgatc accgtcgtaa tctcggtcat acaacagctt
    20461 ccggagcgat taaagatcag atctcaggtt acgaaggcgg tgccaagagt gcccaacctg
    20521 catgggacgc ttatatgaaa gccgttcttg aaggtgtgcc ggagcagccg ctgacgccgc
    20581 cgccgggtat tgtgacggtg aatatcgatc gcagcaccgg gcagttagct aatggtggca
    20641 acagccgcga agagtatttc atcgaaggta cgcagccgac acaacaggcg gtgcacgagg
    20701 tgggaacgac cattatcgat aatggcgagg cacaggaatt gttctgatta aaaaggcgct
    20761 tcagcgcctt tttaagtttg ctgacaaagt gcactttgtt tataccggat acggcgtgaa
    20821 cactttaccc gtctaaaaac atctagaaaa atcaatatat tgcaggagcc acacaggcct
    20881 gataagcatt atgcatcagg caaacttcac gcatttacaa ccgcccctgc cccttcaacc
    20941 attcgcgcac gaggaaaagc gcgctgacat tacgcgcttc gttgaagtca gggtcttcca
    21001 gcaaatccat catatgcgcc agcggccagc gcacctgtgg tagcggctct ggctcatcgc
    21061 cttccagcga ttccgggtag agatcttgcg ctaccacgat attcattttg ctggaaaagt
    21121 aagacggtgc catgctgagc ttcttcaaaa aagtcagatc gttcgcacca aatcccacct
    21181 cttcttttag ctcgcggttc gcggcttcga agacgctttc acccggatca attaatcctt
    21241 tcgaaaaacc taattcgtag gattcagttc ccactgcgta ttcgcggatc aggatcaggt
    21301 gatcgtccac aatcggcaca atcatcactg cttcccggtt ggttggacgc attcgttcat
    21361 aaacacgccg cacgccattg ctgaactcca gatccacgct ttcgacggta aacagtcggg
    21421 aacgggctac agtttcaaca ttcagaatgg tgggtttttg taatgatttg ctcatcgtgg
    21481 gatctatgct gtgaaatcag cggttattgt gcgatatcgg acacgctttc ggcaatgtga
    21541 attgcatgtt atttacattt atgtaactta ataaataatc gtcctcaaat caaattaaaa
    21601 gtcaataggt tgaaataact ccaggaattt gctgatattc cgccttcaga gggtttgcta
    21661 tgatcagcgg ttactgtgat gtgcttaatg atgctcaagt taaactccac gcttgccgat
    21721 agccaaccgc agaatcatgt tttgtgtccg gtgcgactga ccacgcctga cagactaagt
    21781 aagatgggga aagcatgagc accattgtga tttttttagc tgctttgctg gcctgctcac
    21841 tacttgcggg atggctgata aaagtgcgat ccagacggcg tcagctgccc tggaccaacg
    21901 ccttcgcgga tgcgcaaacg cgtaaactca cacctgaaga acgtagcgcc gttgaaaatt
    21961 atcttgagag cctgacgcag gtattacagg tgcctggccc aacgggagcc agcgcggcac
    22021 cgatttctct ggcgctgaat gccgaaagca acaacgtcat gatgctaaca cacgctatca
    22081 cgcgttacgg catctctacc gacgatccga ataaatggcg ttactacctc gattcggtag
    22141 aagtccacct gccccctttc tgggagcagt acatcaacga tgagaatacc gttgaactga
    22201 ttcataccga ttcgctgccg ctggttattt cactcaacgg tcatacgctg caggagtaca
    22261 tgcaggaaac tcgcggctat gccttgcaac ctgttccgtc aacacaggcg tcgattcgcg
    22321 gggaagagag tgagcaaatc gagctactca atattcgcaa agaaacgcac gaagagtatg
    22381 cgctaagtcg tccgcgcggg ctgcgcgaag cgttgctgat tgtcgcctcc ttcctgatgt
    22441 tctttttctg cctgattacc ccggatgtat ttgttccgtg gctggcaggc ggcgcgttac
    22501 tgctgctggg cgcaggtctg tgggggctat tcgcgccccc ggcaaaatcc tccctgcggg
    22561 aaattcattg tctgcgcggt acaccccgtc gttggggatt gtttggcgaa aacgatcagg
    22621 aacagatcaa caatatttca ctcggtatta tcgacctggt ctaccccgca cactggcagc
    22681 cctacattgc tcatgatctc ggccaacaaa ccgatatcga tatctatctc gaccgccacg
    22741 tagtgcgtca ggggcgatat ctttcgctgc atgatgaagt aaaaaacttt ccgttacagc
    22801 actggctgcg cagtacgatt atcgctgcgg gttcgctgct ggtgctgttt atgctgttat
    22861 tctggatccc gctggatatg ccgctgaaat tcactctctc atggatgaaa ggcgcgcaga
    22921 ccattgaagc caccagcgtg aaacaactgg ctgacgctgg cgtacgggtt ggcgatacat
    22981 tgcgtattag cggtacggga atgtgtaata ttcgcacttc cggtacctgg agcgcgaaaa
    23041 ccaactcacc ttttttaccg tttgactgct cgcagatcat ctggaatgac gcccgctcat
    23101 tgccattacc agaatctgaa ttggtcaaca aagcgacggc attgaccgaa gcagttaatc
    23161 gccagctgca ccctaaaccg gaagatgaat ctcgcgtcag cgcctcatta cgttcagcaa
    23221 ttcaaaaatc cggcatggta ttgcttgatg attttggcga catagtactg aagacagcgg
    23281 atctgtgttc tgccaaagat gactgtgtgc gactgaaaaa tgcgctggtc aatctcggca
    23341 acagtaaaga ctgggacgcg ctggtaaaac gcgccaacgc cgggaaactc gatggcgtga
    23401 atgtgttatt acgcccggtg agtgcggaat cgctggataa cctggtggca acctccaccg
    23461 cgccgtttat cacgcatgaa acggcgcgag cggcacaatc actaaacagt ccggcccccg
    23521 gcggattcct gattgtcagc gacgaaggca gcgattttgt tgatcagccc tggccttcgg
    23581 catcacttta cgactacccg ccgcaagaac agtggaacgc tttccagaaa ctggcacaaa
    23641 tgctgatgca tacgccgttt aacgccgagg gtatcgtcac aaaaatcttc actgacgcca
    23701 atggtacgca gcatataggc cttcatccga tcccggatcg ttccggcctg tggcgctatc
    23761 tcagcaccac gctgctgcta ctgacgatgc tgggtagcgc catttataat ggcgtacagg
    23821 cctggcggcg ttaccagcgt catcgtactc gcatgatgaa gattcaggcc tattatgaaa
    23881 gctgcctgaa cccacaactg atcacccctt cagaaagcct tatcgaataa cacgtttgcg
    23941 cggcaggtta tgctaccctg tcgcgcaaat tgcttcactc tggagatttc cctcatgcat
    24001 atcaacattg cctggcagga cgtagatacc gttctgctgg atatggacgg cacgttgctc
    24061 gacctcgcct tcgataacta tttctggcaa aagctggtgc ctgaaacatg gggcgcgaaa
    24121 aacggggtta cgccacagga agcgatggaa tatatgcgcc agcaatatca cgacgtacag
    24181 catacgctaa actggtactg tcttgattac tggagtgagc aactgggtct ggatatctgt
    24241 gcgatgacca ccgagatggg accgcgcgcc gtactgcgtg aagataccat tccgtttctt
    24301 gaggcactga aagccagcgg taagcagcga attttgctca ccaatgcgca tccgcacaac
    24361 ctggcggtaa aacttgagca taccggtctg gacgcacacc ttgatttatt actttccacc
    24421 cacacatttg gttatccgaa agaggatcag cggttatggc atgcggtggc cgaagctacg
    24481 ggtctgaaag ctgaaagaac gctgtttatt gatgacagcg aagcgattct cgatgctgcc
    24541 gcgcaatttg gtattcgtta ctgcctcggc gtgactaatc ctgactccgg gattgccgag
    24601 aaacagtatc aacgccatcc gtcactgaat gactaccgcc gcctgatccc ctcgctaatg
    24661 tgaaggagac gccatgaaag aaaaacctgc tgttgaggtt cgactggata aatggctatg
    24721 ggctgcccgt ttttataaaa cccgcgcgct ggcccgtgaa atgattgaag gcggtaaggt
    24781 gcattacaac gggcagcgca gcaagccgag caaaatcgtc gagctgaatg ccacgctcac
    24841 tctgcgccag ggaaatgacg aacgcacggt gattgtaaag gcgattactg aacagcgtcg
    24901 ccccgccagc gaggcagcct tgctgtatga agagactgcg gaaagtgtag agaaacgcga
    24961 aaaaatggcg ctggcacgta aacttaatgc cttaaccatg ccgcacccgg accgacgccc
    25021 ggacaaaaaa gagcgccgcg acctgttacg atttaaacac ggcgacagtg aataactgtc
    25081 acctgcaaga gagatgatta tgccgcaaca tgaccaatta catcgctatc tgtttgaaaa
    25141 ctttgccgtt cgcggcgaac tggtaaccgt ttcggaaacc ctgcaacaga tccttgagaa
    25201 ccacgattat ccgcagcccg ttaaaaacgt gctggcagaa ctgctggttg cgaccagcct
    25261 gttaaccgct acgctgaagt ttgatggtga tatcaccgta cagctgcaag gcgacggtcc
    25321 gatgaatctg gcggttatta acggtaacaa caaccagcag atgcgcggtg tggcgcgcgt
    25381 gcagggcgaa attccagaaa atgccgacct gaaaacgctg gtcggcaatg gttacgtggt
    25441 gatcaccatc accccgagcg aaggtgaacg ctatcagggc gtggtgggtc tggaaggtga
    25501 tactctggcg gcctgcctgg aagattactt tatgcgttct gaacagctgc cgacgcgcct
    25561 gtttattcgc accggcgacg tagacggcaa accggctgca ggcggtatgt tgttgcaggt
    25621 aatgcctgcg caaaatgccc agcaggacga ctttgaccac ctggcgacgc taaccgaaac
    25681 catcaaaacc gaagaactgt tgaccttacc ggcaaacgaa gtgttgtggc gtttgtatca
    25741 cgaagaagag gtgacggttt atgatccgca ggatgtggag ttcaaatgca cctgctcgcg
    25801 tgaacgttgc gccgacgcgc taaaaacgct gccggatgaa gaagtcgata gcatcctggc
    25861 ggaagatggc gaaattgaca tgcattgtga ttactgcggt aaccactatc tgttcaatgc
    25921 gatggatatt gctgaaatcc gcaacaacgc gtctccggca gatccgcaag ttcattaatg
    25981 agtatgtccg gcagagaggg ctctgccgga ttttacgacg taatggcctg atgcgcttat
    26041 cagacctacg gattcaatgc aatttatcgc aggtaagata atgtgccgaa tccaataaaa
    26101 atcagatatg cgctgtcatt cattcagatt caaatccagt ctttcgccat ccaccgtaag
    26161 atcgcgccac actttaggct tcgtccccag ttttccatcc tccgcagcca tgagtaattt
    26221 ttctttcgtt attacagccg ggagtgaatt catcgtaatc tctttccatt ctttcccagc
    26281 aggatcaata ccatagataa tgtatctttc atcattaaag gcaaacagga tccgctctgc
    26341 cctgccatca ctattcagat cttgctctac cagcacgcag gcatcttttt caatgcatat
    26401 cattgcgtta tagcgatttt tcatcactgc cgaccagaat atcgtatccg gttttccaga
    26461 acctgaggca ataaatacat tatctgctaa tgctttttcc gatatctgct gctgaagacg
    26521 ttgtactcca tcaaatgcca tcagcagacc ccgcctgcgt ttcgggtctt tcatatactc
    26581 ggcatcgctt ttcagcgact caagcgccgc acgtccatag cgaccgctct gttcgagcat
    26641 atagagactt acctggtctg gcgtgttttt gccgctctga taacgcgcca tatgactgtt
    26701 cacgctaatg cgcatactgt ccagcaccgg agaattaaga agcaccagta ttaccaacac
    26761 caataacgaa accgcaaggt tcactttgcc ctgaagaaca agggggtttt gccctttacg
    26821 ccagacgata ctgacaaaat accccagcga ccataccagt aacaccagca ccgccagcgc
    26881 gccctgcaag cggtcgacag tccagccgta ctgagcgacc cgcagccata acgcccaggc
    26941 ggcgacgaac acatacagcg gagcaaccag caaagcggtt ttaatcaggc aacgcaacgg
    27001 ccccgtccag ggaagagacg ctttttgcgg atcgcggaca atagccatta agatcaattg
    27061 caaaaaggcc agcgttaaca gcaacccagc ggcggagatg tggcgagaaa tcgcgctcag
    27121 gcccgtaaac ggcagggtga taataaacat cagggttagc aatgatacta acggcagcaa
    27181 ccccgtggcg attaacgtga acaacttttg aatagagtcg attaaacgtg actgtgtacg
    27241 cgcgaggatc accgccagtg cggtaaccag acctaacgtg agataaataa accagtcggt
    27301 tgcaaaaaag agcgttttga aaaacgtgat gccaacaagt ttaaacaact ctccccacag
    27361 taaaagtacc agccacgtca ggccattggc gagaaaaatc accagtaata taagtacgtt
    27421 atgccatact gactggtaaa aatagcgata gcggctactg tcattgcgga tgcgaaggct
    27481 ttgttgtatc cacggtagca acaacattgc catcagcagc agatagcaac caaaatccca
    27541 aagcgccttt tctgctctcc aggggttcat gccatcaatt tgccacttta gccaaccgct
    27601 catccctagc gtggcaataa acaccagcgc cagccatccc caaaggcgct tctgtttaaa
    27661 ggagatcacg gagaagagta aaacggatga aaacgcgacg gtcgcaggca cgccataaac
    27721 aatccaactg tgattttttc cggcaagcca ggcgatcagc aggtagcaaa ccagaccctg
    27781 aagtaatccg gtagcaatca tcccccagcg tgttgccggt gaaagttcaa cgttatccat
    27841 gttagttatc cagaatcaaa aagtgggtta attattgcat ccgggccgta gtattttgct
    27901 tttttcagaa aataatcaaa aaaagttagc gtggtgaatc gatactttac cgattgaatt
    27961 tgcatcaatt tcattcagga atgcgattcc actcacaata ttcccgccat ataaaccaag
    28021 atttaacctt ttaagaacat tttccacacc taaaatgcta tttctgcgat aatagcaacc
    28081 gtttcgtgac aggaatcacg gagttttttg tcaaatatga atttctccag atacgtaaat
    28141 ctatgagcct tgtcgcggtt aacaccccca aaaagacttt actattcagg caatacatat
    28201 tggctaagga gcagtgaaat gcgcgttaac aatggtttga ccccgcaaga actcgaggct
    28261 tatggtatca gtgacgtaca tgatatcgtt tacaacccaa gctacgacct gctgtatcag
    28321 gaagagctcg atccgagcct gacaggttat gagcgcgggg tgttaactaa tctgggtgcc
    28381 gttgccgtcg ataccgggat cttcaccggt cgttcaccaa aagataagta tatcgtccgt
    28441 gacgatacca ctcgcgatac tttctggtgg gcagacaaag gcaaaggtaa gaacgacaac
    28501 aaacctctct ctccggaaac ctggcagcat ctgaaaggcc tggtgaccaa acagctttcc
    28561 ggcaaacgtc tgttcgttgt cgacgccttc tgtggtgcga acccggatac tcgtctttcc
    28621 gtccgtttca tcaccgaagt ggcctggcag gcgcattttg tcaaaaacat gtttattcgc
    28681 ccgagcgatg aagagctggc aggtttcaaa ccagacttta tcgttatgaa cggcgcgaag
    28741 tgcactaacc cgcagtggaa agaacagggt ctgaactccg aaaacttcgt ggcgtttaac
    28801 ctgaccgagc gcatgcagct gattggcggc acctggtacg gcggcgaaat gaagaaaggg
    28861 atgttctcaa tgatgaacta cctgctgccg ctgaaaggta tcgcttctat gcactgctcc
    28921 gccaacgttg gcgagaaagg cgatgttgcg gtgttcttcg gcctttccgg caccggtaaa
    28981 accacccttt ccaccgaccc gaaacgtcgc ctgattggcg atgacgagca cggctgggac
    29041 gatgacggtg tgtttaactt tgaaggcggc tgctacgcaa aaactatcaa gctgtcgaaa
    29101 gaagcggaac ctgaaatcta caacgctatc cgtcgtgatg cgctgctgga aaacgtcacc
    29161 gtgcgtgaag atggcactat cgactttgat gatggttcaa aaaccgagaa cacccgcgtt
    29221 tcttatccga tctatcacat cgaaaacatc gttaagccgg tgtccaaagc gggccacgca
    29281 actaaagtta tcttcctgac cgcagacgcc tttggtgtgc tgccgccggt ttctcgtctg
    29341 actgccgatc aaacccagta ccacttcctc tctggcttca ccgccaaact ggccggtaca
    29401 gagcgcggca tcaccgaacc gacgccaacc ttctccgcct gcttcggcgc ggcattcctg
    29461 tcgctgcacc cgacgcagta cgcagaagtg ctggtgaaac gtatgcaggc ggcaggcgcg
    29521 caggcgtatc tggttaacac cggctggaac ggcactggca aacgtatctc cattaaagat
    29581 acccgcgcca ttatcgacgc catcctcaac ggttcgctgg ataacgcaga aaccttcact
    29641 ctgccgatgt ttaacctggc gatcccaacc gaactgccgg gcgtagacac caggatcctc
    29701 gatccgcgta acacctacgc ctctccggaa cagtggcagg aaaaagctga aacgctggcg
    29761 aaactgttta tcgacaactt cgataaatac accgacaccc ctgcgggtgc cgcgctggta
    29821 gcggcaggtc ctaagctgta atcagctgat atctcattga ccccttatcc aaacggataa
    29881 ggggtttttt tatctaatta gctaccaata tgtacacggt acacttataa ttatcaatag
    29941 accgcaggat gcattatgtt cacctttatt gaactacaag gatttagcaa acgccgccag
    30001 ttattgctac ctgatgatga gttccgggct tttcaggaac tactcattca agatcccact
    30061 gccggtgaca ttatcgcagg cacaggcagt tttagaaaaa ttcgctggaa ccgttctgga
    30121 atggggaaac ggggtggcat tcggataatt tattacaacg taacacgcaa tggccgaatt
    30181 tatctggcgt tgatttatcc taaaaatgaa caggatgatt taacagaaga acaaagaaag
    30241 gcattgaaac tattgtccga gaagctgctt taatgttgaa gctccacaaa ttccaccatg
    30301 gccgaaatgt caacggaggc attgaaaaca gatgaaagat gaattatttg ctgatcttct
    30361 tgccagcgca gaagaaatgg tacgcatcga aaagggtgaa gaaacgcctg aaccagagca
    30421 cgtccatact tttagcgaaa tcgatgtaaa agcgatccgt gaagcaacgg gtttacgtca
    30481 gcaagatttt gctgtcgccg taggggtaag ttatgacctg gtcaaaagct gggaaaccaa
    30541 acgccgtcaa cctaccggag caccccgaaa attgctcctg cttttgcaag cgaatccttt
    30601 cattatcaat caattgaaaa caatctaaaa acaggaggca ccttcgcctc ccgttttatt
    30661 tacccttctt ttgtcgtgcc ctgcgcccgc gttaccggca ctggcagcca ggcgcgaatg
    30721 gaaagcccgc cccgctcgct gctgccaagc tccagcatgc cgttatggtt atccacgata
    30781 cgctgcacaa tcgccagccc taatcccgtg ccgctaatgg tgcgcgcact gtcgccgcga
    30841 acaaacggct ggaacaggtg cttacgttgt tccggcgcaa ttcccggacc atcatcttcc
    30901 acctggaacc aggcgcgatt cggctccgtt ccgctgctga ctttgatcca gccattgcca
    30961 tagcgggcag cgttcaccac catattcgcc accgcacgtt tgatcgacag cgggtgcatt
    31021 ttcacctcaa tgctgccggg gtaaagcgcg gtttcaattt cccgctcata gccactttcg
    31081 gcggcaatca cctcaccgag cactgcgttg agatccgcca tttccatcgg catctcctgc
    31141 ccggtgcgca ggtagtcgat aaactgctca atgatggcgt tgcactcttc gatatcttta
    31201 ttgatcgatt cggccaggta gccatcctgc tcgctcatca tctcggtcgc caggcgaatg
    31261 cgcgtcagcg gcgtacgcag atcatgactt acccccgcca tcagcagcgt gcgatcatcc
    31321 gccagttgct taacaccagc cgccatatgg ttaaaggcgc gggtaacgga acgcacctcc
    31381 gaagcgccat attcacgcag cggcggcgga ataatccctt taccgacctg cagagcagca
    31441 tgttcgagat cgaccaacgg tcggttctgg atacggataa acagccacgc cccgcctatc
    31501 gccaatagca taatcgccag cgtatagcgg aacagcggag aaaaatcacc ctgatgaatt
    31561 tcggtcagcg gcacgcgcac ccagatattg ggcgacagcc aggttttcag ccagacaaca
    31621 ggcgaacttt tgttgacctc aacgcgcact tccgtcgggc cgcccagttg ctgcgccatc
    31681 tgatggctta agaattcata gtgttgtgcc caacgcagac ctgcctcttc ggcagcttcg
    31741 ttggaataga gagagatccc cagctcacgg tagatctccc gacggaaagc gggaggcaca
    31801 accaactgcg tgccgtcctc cagttgcagt ttgtcggtca tcaacatacg cacttcgtac
    31861 gcgaggactt tattaaactg ctggaggctc ggcaaaatcg cgaagttcag caccaccaga
    31921 taagtcgtca ccaggctgac gaacagcaag gtgacgatga gcaataacgt acgggcaaat
    31981 gaacttcgtg gcgagaagcg caatcgcctc atgctttaga accgtccggt acaaagacgt
    32041 agcccagacc ccagacggtc tgaatgtaac gcggatgcgc cggatcttct tccaccatgc
    32101 ggcgtagacg cgaaatctgc acgtcgatgg agcgttccat tgcggagtat tcacggccac
    32161 gggcaaggtt catcagctta tcgcgggaga gcggctcacg cggatggctg accagtgcct
    32221 tcagtaccgc aaactcaccg ctggtgagcg gcatcggctc atcttcgcgg aacatttcac
    32281 gcgtaccgag gttaagtttg aacttaccga aagcaattac cgcctcttcc tgtgacggcg
    32341 cgcccggcag ttcgttggcc tgacgacgca gcaccgcacg aatgcgggcc agcaattcac
    32401 gcgggttaaa cggttttgga atgtagtcgt cagcgccaat ctccaggcca acgatacggt
    32461 ccacttcttc ccctttcgcc gtcaccataa tgatcggcat cgggttgctc tggctacgaa
    32521 gacgtcggca aatcgacaag ccatcttcac caggtaacat caaatccagt accataagat
    32581 ggaaagattc acgagtcagc aggcgatcca tctgttctgc attagcgacg cttcgaacct
    32641 ggaagccttg ttcggtgaga taacgttcca gcagcgcgcg caggcgcata tcgtcatcga
    32701 ccaccagaat cttgtagttc tcttgcattg tttgtactcc caaaggttcg caacaatttg
    32761 taagcgtgta ttcttaaaaa agctcacgtt cgtcaccagc taaatctggt atgaatttca
    32821 gcctaaattg ttacaaagca tattaaacag cagcttaagt atacaattta ttcggcgaaa
    32881 cattattgat tctgttgata tgatcacgtt atacccaatg tgcgcattat caaacagaca
    32941 aagggaatca acgagatgaa aacgcccctg gttacccggg aagggtatga aaaactcaaa
    33001 caagagctta attatctctg gcgtgaagaa cgcccggagg tcacaaaaaa ggtgacctgg
    33061 gccgcaagtc tgggcgaccg cagcgaaaat gctgactatc agtataataa aaagcgcctg
    33121 cgtgaaatcg accgtcgcgt gcgctatctc actaaatgca tggaaaatct caaaatcgtc
    33181 gattactccc ctcagcagga aggcaaagtc ttttttggtg cgtgggtgga gattgaaaac
    33241 gacgatggcg tgactcaccg tttccgtatt gtcggctacg atgaaatttt tggccgtaaa
    33301 gattacatct ctatcgattc cccgatggct cgcgcattgt tgaaaaaaga ggtgggcgat
    33361 ttggcggtgg tgaatacccc tgccggggaa gcgagctggt atgtgaatgc tatcgagtac
    33421 gtgaaaccgt aaggaagagt cttaacctcc tgccgatggc tggcattttt gccagccagt
    33481 ccgtataact atcccctgat ttttgatccg aaaagatgaa ctcaaaccat gatgaatgat
    33541 tcgttctgcc gcattattgc gggtgaaatt caggcgcgcc cggaacaggt tgacgctgcc
    33601 gttcgcctgc ttgacgaagg gaataccgtg ccgtttatcg cacgttatcg taaggaaatc
    33661 accggcggtc tggatgacac gcagctgcgt aatctggaaa cgcgtctgag ctatctgcgc
    33721 gagctggaag agagacgtca ggcgatcctc aagtccattt ccgagcaagg caaactcact
    33781 gacgagctgg cgaatgccat caacgccaca ctaagcaaaa ccgaactcga agacctctac
    33841 ctgccctaca aacctaaacg ccgcacccgc gggcaaatcg ccattgaagc agggcttgag
    33901 ccgttggctg acctgctgtg gagcgatccg tcacacacgc cagaagtcgc cgctgcacaa
    33961 tatattgatg ccgataaagg cgtggcagat accaaagccg cgctggacgg cgcgcgctat
    34021 atcctaatgg aacggtttgc cgaagatgcc gcgctgctgg cgaaagtgcg tgattatctg
    34081 tggaagaacg cgcatttggt ctcaaccgtg gtgaatggta aagaagagga aggggcgaaa
    34141 ttccgcgact atttcgatca tcacgaaccg ctgtccacgg tgccttctca ccgcgcgctg
    34201 gcgatgttcc gtggtcgtaa cgaaggcgta cttcagcttt cgctgaatgc cgatccgcag
    34261 ttcgaagagc cgccgaaaga gagctattgc gagcaaatca tcatggatca ccttggcctg
    34321 cgcctgaaca atgccccggc ggatagctgg cgcaaaggtg tggtgagctg gacgtggcgc
    34381 atcaaggtgt tgatgcatct ggaaactgaa ctgatgggca ccgtgcgcga acgcgcggaa
    34441 gatgaagcaa tcaacgtctt tgcccgtaac ctgcacgatc tgctgatggc ggcccctgcc
    34501 ggactgcgtg caacgatggg cctcgatccg ggtctgcgta ccggggtaaa agtggcggta
    34561 gtcgatgcga caggtaaact ggtggcgacc gataccattt atccgcatac cgggcaggcc
    34621 gcaaaagcag cgatgaccgt tgctgcgctg tgtgaaaagc ataacgttga actggtggcg
    34681 atcggcaacg gtacagcttc tcgcgaaacc gaacgtttct atctcgacgt gcagaagcag
    34741 ttcccgaaag tgaccgcgca gaaagtgatc gtcagcgaag ctggcgcgtc ggtttactcg
    34801 gcttccgagc tggcagcaca ggaattcccg gatctcgacg tttcgctgcg tggcgcggtg
    34861 tctatcgccc gtcgtttgca ggatccgctg gcggagctgg tgaaaatcga tccgaaatct
    34921 atcggcgtag gtcagtatca gcatgacgtc agccagacgc agctggcacg caaactggat
    34981 gcagtagtag aagactgcgt aaacgccgtt ggcgtcgatc tcaacaccgc ttctgttcct
    35041 ctgttaaccc gcgtggcggg cctgacgcgc atgatggcgc aaaacatcgt ggcctggcgc
    35101 gatgagaacg gccagttcca gaaccgtcag caactgttaa aagtgagccg tctggggccg
    35161 aaagccttcg agcagtgcgc gggcttcttg cgcattaacc atggtgataa cccgctggac
    35221 gcgtctaccg tccacccgga agcctatccg gtggtggaac gcattctggc agcaacacag
    35281 caggcactga aagatctgat gggtaacagc agcgagctgc gtaacctgaa agcgtctgac
    35341 tttactgatg aaaaattcgg tgtgccgacg gtaaccgaca tcatcaaaga gctggaaaaa
    35401 ccgggtcgcg atccgcgtcc ggaatttaaa accgctcagt ttgccgatgg cgtcgagaca
    35461 atgaacgacc tgcaaccggg tatgatcctc gaaggtgcgg tgaccaacgt caccaacttt
    35521 ggcgcgtttg tcgatattgg cgtgcatcag gacggcctgg ttcacatctc ttcattgtcg
    35581 aacaagtttg tggaagatcc gcataccgtg gtgaaagcgg gcgacattgt gaaggtgaaa
    35641 gtgctggaag tggatcttca gcgtaaacgt atcgccctga ccatgcgtct ggacgagcag
    35701 cctggcgaaa ccaacgcccg tcgcggtggc ggtaacgatc gcccgcaaaa caaccgcccg
    35761 gcagccaaac cgcgtgggcg tgaagcgcag cctgccggta atagcgcgat gatggatgcg
    35821 ctggcggcgg caatgggcaa aaaacgttaa acgcccggac tggcccctaa acctgcgcga
    35881 ttaggcgttc acgccacatc tggcaacgaa cgccttgcct gacataaaag tgccggagaa
    35941 tatctccggc ctttttattc cacaagcaaa ctcataatat atcccattaa tatttatcac
    36001 ttcattaaca actgaaacct taattaaaca ttaaccagcc ctaataattc gtcatgcgaa
    36061 ttatattttc gctgcgatat aaccttgagc catatcaaca ttgagtcaga ttattattca
    36121 aaccaacatt cacacacatt ttaagtattg ctgatagaaa ccattctcat tatcattgta
    36181 ctgttgatta tttaatctct ccttcgttgg caaatcatct ggtctcatgt cgctgtcaaa
    36241 cgccccatga ggtagttatc cagttaatga gaaacaagta ggcacctatg caatacactc
    36301 cagatactgc gtggaaaatt actggctttt cccgtgaaat cagcccggca tatcgccaaa
    36361 aactgctttc tcttggcatg ttacctggct cctcttttaa tgtggtgcgc gtcgctccac
    36421 tcggcgaccc cattcatatc gaaacccgtc gtgtgagcct ggtattacga aaaaaagatc
    36481 tggccttatt agaagtggaa gcggtttcct gttaatacgg tgataacaac aatgaaaaaa
    36541 ttaaccattg gcttaattgg taatccaaat tctggcaaga caacgttatt taaccagctc
    36601 actggctcac gtcagcgtgt aggtaactgg gctggcgtta ccgtcgaacg taaagaaggg
    36661 caattctcca ccaccgatca tcaggtcacg ctggtggacc tgcccggcac ctattctctg
    36721 accaccatct catcgcagac ctcgctcgat gagcaaatcg cctgtcacta cattttgagt
    36781 ggcgacgcag acctgctgat taacgtggtg gatgcgtcta accttgagcg taacctgtac
    36841 ctgacgctgc aactgttgga actcggcatt ccctgcatcg tggcactgaa catgctcgac
    36901 attgccgaga agcaaaatat tcgtattgaa attgatgctc tgtcggcgcg tcttggctgc
    36961 ccggtgatcc cgctggtttc aacccgtggt cgcggtattg aagcgctcaa gctggcgatt
    37021 gatcgctata aagctaacga gaatgtggaa ctggtgcatt acgcacagcc gctactcaac
    37081 gaagcagatt cactggcaaa agtgatgcct tccgacatcc cgctgaaaca acgtcgctgg
    37141 ctgggcctgc aaatgctgga aggcgatatc tacagccgcg cctacgccgg tgaagcgtcg
    37201 cagcatctgg atgccgccct cgcccgtctg cgtaatgaga tggacgatcc ggcgctgcac
    37261 attgccgatg cgcgttacca gtgcattgct gccatctgtg atgtggtaag caacaccctg
    37321 acggcagaac ccagccgttt caccactgcg gtagataaaa tcgtgctcaa ccgtttcctc
    37381 ggtctgccga ttttcctctt tgtgatgtac ctgatgttcc tgctggctat caacatcggc
    37441 ggggcgttac agccgctgtt tgatgtcggt tccgtggcgc tgtttgtgca tggtattcag
    37501 tggattggct acacgctcca cttcccggac tggctgacta tcttcctcgc ccagggcctg
    37561 ggtggcggta ttaacaccgt gctgccactg gtgccgcaga tcggcatgat gtacctgttc
    37621 ctctccttcc ttgaggactc cggctatatg gcgcgtgcgg cgtttgtgat ggaccgcctg
    37681 atgcaggcgc tgggcttgcc ggggaaatcc tttgtaccgc tgatcgtcgg cttcggttgt
    37741 aacgtaccat cggtaatggg cgcacgtacg cttgatgcac cgcgtgaacg cctgatgacc
    37801 atcatgatgg caccgtttat gtcctgcggc gcacgtctgg ctatcttcgc agtattcgcg
    37861 gctgccttct tcgggcagaa cggtgcgctg gcggtcttct cgctgtatat gctgggtatc
    37921 gtaatggcgg tgctgacggg cctgatgctc aagtacacca tcatgcgcgg tgaagcgacg
    37981 ccgtttgtca tggagctgcc ggtctaccat gtaccacacg ttaaaagcct gattatccag
    38041 acctggcagc gtctgaaagg cttcgttctg cgtgctggta aagtgattat catcgtcagc
    38101 attttcctga gcgccttcaa cagcttctcg ctgagcggaa aaatcgtcga taacatcaac
    38161 gactcggcgc tggcttccgt cagccgggtg atcaccccag tcttcaagcc gattggcgtg
    38221 catgaagata actggcaggc aacggttggc ctgtttacag gcgcaatggc gaaagaagtg
    38281 gtggtgggca cgctcaacac cctctacacc gcagaaaata ttcaggacga agagttcaat
    38341 ccggcagaat ttaacctcgg tgaagagctg ttcagcgcgg tagatgaaac ctggcagagc
    38401 ctgaaagaca ccttcagcct tagcgtactg atgaatccca ttgaagcgag caaaggcgac
    38461 ggcgaaatgg ggaccggggc gatgggcgtg atggatcaga aattcggtag cgcagctgcc
    38521 gcatacagct acctgatttt cgtcctgctg tatgtaccgt gcatctcggt gatgggggct
    38581 atcgcccgtg aatcaagccg tggctggatg ggcttctcca tcctgtgggg gctgaatatc
    38641 gcttactcac tggcaacatt gttctatcag gtcgccagct acagccagca cccaacttac
    38701 agcctggtgt gcattctggc ggttatcctg tttaacatcg tggttatcgg tttgctgcgt
    38761 cgcgcgcgta gccgggtgga tatcgaactg ctggcaacgc gcaagtcggt aagcagttgc
    38821 tgcgcagcca gcaccaccgg tgattgccat taatggcttc acttattcag gtgcgtgatt
    38881 tgctggcgtt acggggccgt atggaagcgg cccagataag ccagacattg aacaccccgc
    38941 agccaatgat taacgccatg ctgcaacagc tggaaagcat gggcaaagcc gtgcggattc
    39001 aggaagaacc tgacggctgc ctctctggca attgtaaaag ctgcccggaa ggcaaagcct
    39061 gcctgcgcga gtggtgggcg ctgcgttaac catactccat cacctgtttt gaaaagcccg
    39121 gtatgcgttt gcatccgggc ttttttgcgt gcggctttcc ataaaaatgc aacccttgca
    39181 gcgcgacgta aattcctctg aaagcgtctc gcagggatga aaactcgcta atgcacaggt
    39241 gtggagtggt gcgtagagtc gcggcattca aacaacaggt gaaggaacgc catgagcaaa
    39301 aagcagagtt ccaccccaca cgatgcgctg ttcaaactct ttttacgcca accggagacg
    39361 gcgcgcgatt ttcttgcgtt tcatttaccg gcacctattc acgcgctctg tgatatgaaa
    39421 accctcaagc tggagtcgag cagctttatt gatgacgatc tgcgtgaaag ctattccgac
    39481 gtgctgtggt cggtgaaaac ggaacaaggg ccaggatata tctattgtct gattgaacat
    39541 caaagcacct caaacaaact gatcgcattt cgcatgatgc gttacgccat tgccgcaatg
    39601 caaaatcacc tggatgccgg atacaaaaca ttgccgatgg tggtgccatt gttgttttac
    39661 cacggtattg aaagccccta tccctattcg ctgtgttggc ttgattgttt cgccgatccc
    39721 aaactggcaa ggcagcttta tgcctccgca tttccgctga ttgatgtcac cgtcatgcct
    39781 gatgatgaaa tcatgcagca ccgacgtatg gcgctgctgg agttaattca aaaacatatt
    39841 cgtcaacgcg atctgatggg gctggtagag caaatggcct gcttattaag tagtggatac
    39901 gctaatgaca gacaaatcaa agggctgttt aattacatct tgcaaaccgg cgatgcggta
    39961 cgttttaacg attttatcga cggcgttgcc gaacattcac cgaaacacaa ggagagctta
    40021 atgactattg cggaaagatt gcggcaagag gggtttcaga aaggtatacg agctatcgcc
    40081 aaaaccatgc tggatgccgg agtccctctt gaggacgttc tgcgatttac cagcctgacg
    40141 gttgaagacc ttgctgaaat aaatcatcaa taacgtaaag gatggtttta atgacagatg
    40201 cagaaagaga atatcttatt agttacctga ttggttattt attgggtcga cgcctggaag
    40261 ccctggatat cgccaaaaga atgctgtaat ccggagtccc tcttgcagac atcatgcgct
    40321 ttaccggggt gtcagaagaa gagttggctg cggcgagcca gtaaagttct gtctcgccat
    40381 ttcaaaagcc acctacaccc tctgcttcaa cgccaccagc atgcgacaaa actcggccgg
    40441 atgcgaaata aacggcgcat gggccgcttt ggcgaagatg tatgattcgc tgtgaggcca
    40501 aagtttatcc agcatcggca ccactttgcg cggcaccaga ccgtcgagat agccatacaa
    40561 tcgcaaaaac ggcatggaca cgttttgcag cggctgacgg agatcgacca ttttcaggat
    40621 ttccagcccg ccattaagca cgtcaacctc cggcatcggt aacgccagaa cggttttctt
    40681 cagcgcccgc gcatcctggc gcgccgtttc agtacccatg gtttgtaacg ccaggaaccg
    40741 ctccaccgta cgctgaaaat catcactgag ttgctgctga aatcccgcca gcacgtccgg
    40801 ttttatcccc ggccactcgt cacgagcact aaaacaaggt gacgacgcca cggtgaccag
    40861 cgcctgaaca cgctcgggat gggttaacgc aatctggctt gccaccagcc cgcccagact
    40921 ccagcctaac caaatggctt tatcaggtgc ctgtcgcagc acggcttcgg ccatatcagc
    40981 aagtgacatc gcaccaaatc cccggctacg cccgaagccg ggcagatcaa caaggtgcag
    41041 cgtaaaatgc gagctcagtt cctcgtcaat gcaacgccac acttcggcat tcagtcccca
    41101 tccgtgcagc agcacaagat gaacattccc ctgacctttg gtctgccacc agatgttatt
    41161 catccgctat tgttctcttt tgacttacaa ggatgaacat atgctaacag taccgggatt
    41221 atgctggcta tgccgaatgc cgctggcgtt aggtcattgg gggatttgtt cggtatgctc
    41281 acgcgccgcc cgtacagata aaacgttatg cccccaatgt ggattacccg ccacacactc
    41341 acatcttccc tgcggtcgct gcctgcaaaa accgccgccc tggcaaagac tggtcacggt
    41401 tgccgactat gcgccgccgt taagtccgct tatccaccag cttaagtttt cccggcgcag
    41461 cgaaatcgcc agcgccctgg cacgtctgtt actattggaa gtcttacacg ctcgtcgcac
    41521 caccgggttg caattgccgg atcgcattat cagcgttccg ttatggcagc agcgtcactg
    41581 gcgtcgggga tttaatcaga gcgatttgct gtgtcagccg ttatcacgct ggttgcactg
    41641 ccgatgggat agcgaagccg tcacacgaac acgggccact gcgacccagc attttctcag
    41701 tgcccggctg cgcaagcgca acctgaaaaa tgcctttcgc cttgaattgc ctgtgcaagg
    41761 tcgccatatg gtgattgtgg atgatgtcgt caccaccgga agtaccgtcg cagagattgc
    41821 gcagttgctt ttacgcaatg gtgcggcgac tgtccaggtc tggtgccttt gtcgaacctt
    41881 gtagagcctc gatgatgggc gtattataac caactaaaat agtcaactat taggccatta
    41941 ctatgatccg tatttccgat gctgcacaag cgcactttgc caaactgctg gcaaatcagg
    42001 aagaagggac acaaatccgc gtatttgtga ttaaccctgg cacgcctaac gctgaatgtg
    42061 gcgtttctta ttgtccgccg gacgctgtag aagccaccga cactgccctg aaatttgacc
    42121 tgctgaccgc gtatgttgat gagttaagcg ctccttacct ggaagatgca gagatcgact
    42181 ttgttacaga ccagttgggt tcccagttaa cgctgaaagc gcctaacgcc aaaatgcgta
    42241 aagtcgcaga cgatgcaccg ctgatggagc gcgtggagta tatgctgcag tcgcagatca
    42301 acccacagct tgctggtcac ggtggtcgcg tttcgctgat ggaaatcacc gaagacggtt
    42361 acgccattct gcaatttggc ggcggctgta acggttgttc tatggtagat gtgacgctga
    42421 aagaagggat cgagaagcag ctgctgaacg aattcccgga gctgaaaggt gtgcgcgatc
    42481 tcaccgaaca ccagcgcggc gagcactcct actactaagt tatcccctct tttgtagatt
    42541 gcccgatgcg acgctaaagc gtcatttcgg gcctacgttg aacatgtgcc agacgttggc
    42601 gcagcattgc tttggcaaac ccctcccttc gtttccccgc gataatatga ccaacctctc
    42661 ataatttaaa tttaccccgc tctggtgatt ctcaaacgcc ggatgttacc cgtatcattc
    42721 acgtgggtac caaacatact cctgacatct gactacaata attagtttta gtgggtatca
    42781 gtcgtggtgc cgcaatatct ctgttcccga ttgggataat tagagtttgt cgtcagaaaa
    42841 ttgacgttac ccataacaaa tgaaaggcca ggtaaatcat gccattagtc attgttgcta
    42901 tcggtgtaat cttgttgttg ctcctgatga tccgtttcaa aatgaacggc ttcatcgctc
    42961 tcgtcctcgt ggcgcttgct gttggattaa tgcaaggaat gccgctggat aaagttattg
    43021 gctccatcaa agcaggtgtc ggcggaacgc tcggtagcct tgccctgatc atgggttttg
    43081 gcgcaatgct gggcaaaatg ctggcagact gcggtggcgc acaacgtatc gccaccacgc
    43141 tgattgctaa atttggtaaa aaacacatcc agtgggcggt ggtactgacc ggttttaccg
    43201 ttggttttgc cctgttctat gaagtgggct ttgtgctgat gctgccgctg gtgtttacca
    43261 tcgcggcttc cgcgaatatt ccactgctgt atgttggtgt accaatggcg gctgcactgt
    43321 ctgtgaccca cggcttcctg ccaccgcatc cgggcccgac tgcgattgcc accattttca
    43381 atgccgatat gggtaaaacc ctgctgtacg gtactattct ggcaatcccg accgtgattc
    43441 tcgccggtcc ggtttacgct cgcgtgctga aaggtataga taagccgatc ccggaaggtc
    43501 tgtacagcgc gaaaaccttc agcgaagaag agatgccaag ttttggcgtc agcgtctgga
    43561 cctctctggt cccggttgtg ctgatggcga tgcgtgcgat tgccgaaatg atcctgccga
    43621 aaggtcacgc tttcctgccg gtagcggagt tcctcggtga cccggtaatg gctacgctga
    43681 ttgccgtact gattgcgatg ttcacctttg gtctgaaccg tggtcgttca atggatcaga
    43741 ttaacgacac gctggtttct tccatcaaaa tcattgcgat gatgctgttg attatcggtg
    43801 gtggcggtgc gttcaagcag gtgctggtag acagcggcgt ggacaaatac attgcttcca
    43861 tgatgcacga aaccaacatt tctccgctgc tgatggcctg gtcgattgct gccgtactgc
    43921 gtatcgcgct gggttccgca accgttgcgg caatcactgc gggtggtatc gcggcaccgc
    43981 tgattgcaac gacgggtgtt agcccggagc tgatggttat tgcggttggt tccggtagtg
    44041 tgattttctc tcacgtgaac gatccgggct tctggctgtt caaagagtac tttaacctga
    44101 ctatcggcga gaccatcaaa tcctggtcga tgctggaaac cattatctcg gtgtgcggtc
    44161 tggtaggctg tctgctgctg aatatggtga tttgataaca ctgactgccg gatgcatcgc
    44221 atccggcatc aaccgcactc tacttcttct tcgctgccgc tctgcgccgt ctgtccaaat
    44281 ccttcagcaa cttgttcaca ccatcatcgg caaacatcgc ctcaagcgtt gcggagagct
    44341 tgcgccgcca gtttttatac tggtaactgg tgccgggaat atttaccggg tccgccatat
    44401 ccagccagtc ttctggctgt agtcctaaca gggcgctgtt gctatcggcg atgtagcgct
    44461 gtaagccacg attcagcgtc ggtgtcatcg acatcaacga tgccttatgc ccggcacgtt
    44521 ttggcaggca accatattta tgcagagcat ccagtagtcc ttgcttcgcc agttcgcgat
    44581 cctgatacag accgcgcagt accacttcat ccggatacag ccccagcgtt ttgcccagcg
    44641 ttaaatcccc gctctcccag tagccgcgta gcgtaggcag gtcgtgtgtc gccgcaaccg
    44701 ccatcgactg ctctgggtac gctttcggtg cgcggaatgt cttctcgtgg tcgttttcga
    44761 aatagagcac tttgtaggag tacacaccgc tgctgcgcag cttaccgaca atctctaccg
    44821 gtacggtacc gagatcttca ccaatcacca tacagcgatg acgtttactt tcgagtgcca
    44881 gaatcgagag cagatcatcc accggatagt gaacatacgc gccctgatct gccgtctcgc
    44941 catacggtat ccaccacaaa cgcagcatcg acatcacgtg atcaattcgt aatgcgccgc
    45001 agttttgcat attggcacgc aacagctcga taaacggttc ataggcacgc gcggtgataa
    45061 tatgcgggtc cattggcggc aatccccagt tctgccccaa cgggccgagg atatccggcg
    45121 gtgctccaac cgacgctttc aggcaataca actcccggtc gcaccaggtt tctgatccac
    45181 cctctgctac accaaccgcc agatcacgat acaagccaat cggcatttca tagccctggc
    45241 ttatctccca gcaggcggca aactggctgt aagccagcca ctgcaaccag agataaaaat
    45301 cgacgtcatc acgatgttct tcgcagaact ggcgcacttc tggtgaatcc acgttctgat
    45361 acatctctgg ccatgcaggc cagccccagc gcatttcgtc ctctttcact tgctgggcat
    45421 gtagcgcgtc aaacgccgcc tgccagaaca ggctatcgcc ttgctcggca acaaactgac
    45481 gaaacgcggc cacctgttca tcatcacgtt gcgcgaaacc tttccacgcc atgcgcaatg
    45541 ctgtcatttt tagggcggta accgtggagt aatcgaccca gtcggcatcc cgcgtctgtt
    45601 gcagcgtctg ttgcgtggtc ggcaacttcc accaggcctg agcctcttcg ctaagatgga
    45661 aatcttcgac ggcgttaacg tcgatataaa tcacattcag ccaacggcga gaagacgggc
    45721 tgtatgggct ggcactttcc ggatttgccg gatagagcgc atgaatcggg ttcagaccga
    45781 tgaacgaccc gccacgtttt gccacatcca ccagcatcgc tttgagatcg ccaaaatccc
    45841 caatacccca gtttttttcc gatcgcagcg tataaagctg aacgcaggca ccccacagct
    45901 tttgtttatt cagcaacgcc tgcggttcgt aacagcgttt cggggcgaca atcaccctgc
    45961 aatgcacgcg ctggtcttcc tgggtaaacg tcagcgtgtg ataaccttcc ggcagcttcg
    46021 tcggtagatt gaacgctttg ccccccgtta catggccttt gtactgcgtt ccttcttcgg
    46081 tggtcagcag ccagctatat tcgccgctgc cctccaccac catcggcatt tttttgccgc
    46141 tggtataaac catgacattc gggactggcg ttaccgccac tttcgtggcg gtacgttgat
    46201 gcatcgcgtc aagcaaacgc cgtttggttt cggcgctaat cgactgcggt ttaccgtggg
    46261 cattgatgta attggggcta atccccgccg ccagcgcggc attatccaga cgtttgcttt
    46321 ccatcgagct tccttagcgt tttgcctgcc agatacgagc ctgataatcg cgaatagagc
    46381 gatccgagct aaacataccg cagcgggcgg tattgaggat cgccgcgcga gtccaggcct
    46441 cctggtcgcg gtacagcaca tccacctgct tttgtgcctc tacataggct gcgaaatccg
    46501 ccatcaccag atacggatcg ccgccctgtt tgccgatact gtgcagcatc tgatcgaagg
    46561 catgcttatc gccgtcgctg tatttgccgc tttccagctc tttcaatact gcgtccagca
    46621 ccttatcttt cttccgccat ttcaccgggt cgtagccttt ggccagaatt gccttcactt
    46681 gttccacggt atgaccaaaa ataaagatat tttcttcacc aactttctcg gcgatttcaa
    46741 cgttcgcacc atccagcgta ccgacagtaa gcgcaccatt gagcgccagt ttcatattgc
    46801 cggtaccgga agcttcttta cctgccgtcg aaatttgttc ggagatatcc gccgccggga
    46861 tcagtttttc cgccgccgaa acgcaataat ccggcaggaa caccaccttc aatttgtcgc
    46921 caacctgcgg atcgttgttg atcacgtcag ccactttgtt gatcgcaaag ataatgtttt
    46981 tcgccaggta gtagcccggt gccgctttcg cgccaaagag gaagacgcgc ggtacgcgat
    47041 cagcctgcgg gttttcacga atttctttgt acaacgccag aatatgcagc agattcaggt
    47101 gctggcgttt gtactcatgc agacgtttga tctgaatatc gaaaatcgcc tgtggattga
    47161 tctcaatacc ggtacgaact ttcacaaact ctgccagacg gactttattc gcctgcttga
    47221 tctcgcgata ttgctgacgg aatttcgcat catcagcgaa tttttccaga ttgatcagtt
    47281 gatcgagatc gttagtccac tcttttttca gtgatttatc caacagagcc gccagtgccg
    47341 ggttgcactg tttgatccag cgacgtgggg taataccgtt ggtgacgtta tggaatttgt
    47401 tcggccatag ctggtgatat tccgggaaca gatctttcac caccagatcc gagtgcaggg
    47461 ccgcaacacc gtttaccgcg aaaccgccaa ccacacacag gttcgccata tgcacttgtt
    47521 tgtcgtgtac caccgccagt ttggcccata ctttttcatc gcccggccag gttttttcca
    47581 ccagcgtttt aaagcgtgta ttaatttcgt taataatctg catgtggcgc ggcagtaagc
    47641 ctttcaccag tttcacatcc cagcgttcca gcgcttctgg catcaaggta tggttggtgt
    47701 aagcgaaagt tttactggtg atggcccagg catcatccca gctcatctgg tgctcatcga
    47761 tcagcacgcg cagcagttct ggaatcgcga tagttgggtg ggtatcgttc agctgaataa
    47821 cttcgtactc cgccagctcg tgcagtttac gccccgccag atgatggcga cgcaaaatat
    47881 ccgctaccga gcaggcacac tggaagtatt gctgcatcag gcgcagcttt ttaccggcag
    47941 tatggttgtc gtttggatag agaactttgg tcagtttttc cgcgttgatg ccctgctgtt
    48001 cggcacgcag gaaatcacca tcgttaaatt tagtcagatc aaacggatgc gcatgcgtcg
    48061 cctgccacag acgcagcggc tgcgccacgc cattacgata gccgacaacg gggagatccc
    48121 acgcttgacc ggtaatggta aactccggct cccagcgtcc gtctttcgtc actttaccgc
    48181 caatccctac ctgcacatcc agtgcttcgt tgtggcggaa ccacgggtag ttactgcgat
    48241 gccagtcatc cggcgcttca acctgtttgc catcgacaaa tgactggcgg aacaagccat
    48301 attgataatt aaggccgtaa ccagtagctg actgcccgac agttgccatt gagtcgagga
    48361 agcacgccgc cagacgtccc agaccaccgt tacccagcgc cgggtcgatc tcttcttcca
    48421 gcaagtcagt caggttgatg tcataagcct tcaacgaatc ctgtacatcc tgataccagc
    48481 cgagattcaa cagattgttg cccgtcaggc gaccaatcaa aaactccatt gagatgtagt
    48541 taacatgccg ctgattcgcc accggtttgg caaatggctg agcacgcagc atttcggcca
    48601 gtgcttcgct cactgccaac caccactgac gaggggtcat ttcagccgca gaatttaagc
    48661 cataacgctg ccactgacgt gaaagcgctt cctgaaattg cttatcgtta aaaataggtt
    48721 gtgacatagg agttccactt ttcttagatt ttcaacacaa cgttatcgct agtttgccag
    48781 gctcgatgtt gaccttcctc atcctgcggg ggattaggca gggaggagtt gcggggatga
    48841 gcaaggaaat gtgatctcga ccacttaaag ctagtgcaaa ccacaggatt agcagcaaat
    48901 caatgccata cagcgcagaa aatctgtatc aaagtgcaaa aaatggccgt tgcgtatttt
    48961 caaaaagcgg aaggtaactc tataattaag taaaggagtg aaacagtttc acaagtaaaa
    49021 tatccagtgt gctccatctc attcttaata gatttattaa gatcatcttt ttagatggca
    49081 ctttcatcag aaatgaagaa gaaacccttg cttaaatgaa actgatgaac ataaggaaaa
    49141 taccgtacaa cccagtattc acgctggatc agcgtcgttt taggtgagtt gttaataaac
    49201 atttggaatt gtgacacagt gcaaattcag acacataaaa aaacgtcatc gcttgcatta
    49261 gaaaggtttc tggccgacct tataaccatt aattacgaag cgcaaaaaaa ataatatttc
    49321 ctcattttcc acagtgaagt gattaactat gctgattccg tcaaaattaa gtcgcccggt
    49381 tcgactcgac cataccgtgg ttcgtgagcg cctgctggct aaactttccg gcgcgaacaa
    49441 cttccggctg gcgctgatca caagtcctgc gggctacgga aagaccacgc tcatttccca
    49501 gtgggcggca ggcaaaaacg atatcggctg gtactcgctg gatgaaggtg ataaccagca
    49561 agagcgtttc gccagctatc tcattgccgc cgtgcaacag gcaaccaatg gtcactgcgc
    49621 gatatgtgag acgatggcgc aaaaacggca atatgccagc ctgacgtcac tcttcgccca
    49681 gcttttcatt gagctggcgg aatggcatag cccactttat ctggtcatcg atgactatca
    49741 tctgatcact aatcctgtga tccacgagtc aatgcgcttc tttattcgcc atcaaccaga
    49801 aaatctcacc cttgtggtgt tgtcacgcaa ccttccgcaa ctgggcattg ccaatctgcg
    49861 tgttcgtgac cagctgctgg aaattggcag tcagcaactg gcatttaccc atcaggaagc
    49921 gaagcagttt tttgattgcc gtctgtcatc gccaattgaa gccgcagaaa gcagtcggat
    49981 ttgcgatgac gtttccggtt gggcgacggc actgcagcta atcgccctct ccgcccggca
    50041 gaatactcac tcagcccata agtcggcacg ccgcctggcg ggaatcaatg ccagccatct
    50101 ttcggattat ctggtcgatg aggttttgga taacgtcgat ctcgcaacgc gccattttct
    50161 gttgaaaagc gccattttgc gctcaatgaa cgatgcactc atcacccgtg tgaccggcga
    50221 agaaaacggg caaatgcgcc tcgaagagat tgagcgtcag gggctgtttt tacagcggat
    50281 ggatgatacc ggcgattggt tttgctatca cccactgttt ggtaacttcc tgcgccagcg
    50341 ctgccagtgg gaactggcgg cggagctgcc ggaaatccac cgtgccgccg cagaaagctg
    50401 gatggcccag ggatttccca gcgaagcgat tcatcatgcg ctggcggcag gcgatgcgct
    50461 gatgctgcgc gatattctgc ttaatcacgc ctggagtttg ttcaaccata gcgaactgtc
    50521 gctgctggaa gagtcgctta aggccctgcc gtgggacagt ttgctggaaa atccgcagtt
    50581 ggtgttattg caggcgtggc tgatgcaaag ccagcatcgc tacggcgaag ttaacaccct
    50641 actggcccgc gctgaacatg aaatcaagga cattagagaa ggcaccatgc acgcagaatt
    50701 taacgctctg cgcgcccagg tagcgattaa cgatggtaat ccggatgaag cggaacggct
    50761 ggcaaaactg gcactggaag agctgccgcc gggctggttc tatagccgca ttgtggcaac
    50821 ctcggtgctg ggtgaagtgc tgcactgcaa aggcgaattg acccgctcac tggcgctaat
    50881 gcagcaaacc gaacagatgg cacgccagca cgacgtctgg cactacgcct tatggagttt
    50941 aatccagcaa agtgaaattc tgtttgctca gggattcctg caaaccgcgt gggaaacgca
    51001 ggaaaaagcg ttccagctga tcaacgagca gcatctggaa cagctgccaa tgcatgagtt
    51061 tctggtgcgc atccgagcac aactgttatg ggcctgggca cggctggatg aagccgaagc
    51121 gtcggcgcgt agcgggattg aagtcttgtc gtcttatcag ccacagcaac agcttcagtg
    51181 cctggcaatg ttgattcaat gctcgctggc ccgtggtgat ttagataacg ctcgtagcca
    51241 gcttaaccgt ctggaaaacc tgctggggaa tggcaaatat cacagcgact ggatctctaa
    51301 cgccaacaaa gtccgggtga tttactggca aatgaccagc gataaagccg ccgctgccaa
    51361 ctggttgcgt catacggcta aaccggagtt tgcgaacaac cacttcctgc aaggtcaatg
    51421 gcacaacatt gcccgtgcac aaatcttgct gggcgagttt gaaccggcag aaattgttct
    51481 cgaagaactc aatgaaaacg cccgcagtct gcggttgatg agcgatctca accgtaacct
    51541 gttgctgctt aatcaactgt actggcaggc cggacgtaaa agtgacgccc agcgtgtgtt
    51601 gctggacgca ttaaaactgg cgaatcgcac cggatttatc agtcattttg tcatcgaagg
    51661 cgaagcgatg gcgcaacaac tgcgccagct tattcaactc aatacgctgc cggagctgga
    51721 acagcatcgc gcacagcgta ttctgcgaga aatcaatcaa catcatcggc ataaattcgc
    51781 ccatttcgat gagaatttcg ttgaacgtct gctaaatcat ccggaagtgc cagaactgat
    51841 ccgcaccagc ccgttgaccc aacgtgaatg gcaggtcctg gggctgattt actccggtta
    51901 cagcaacgag caaattgcag gtgagctgga agtggcggca accaccatca agacgcatat
    51961 ccgcaatctg taccaaaaac tcggtgtcgc ccaccgtcag gccgcggtac aacacgcgca
    52021 gaaactgctg aagatgatgg gatatggggt gtgagtttag ccggataata cgtcggatcc
    52081 ggcttacatc tttatatcat tcaatgctca cccgcgttac gccatccgcc tccaccagac
    52141 taaaccgcac tggcaagaaa cgctccacca ccgcaatatt ggtcagcaga tggcatgagg
    52201 gatgggcgac cgtaaattct cccgcgcccg ccagcgccat cggcagtacc agctggtcgg
    52261 cgagatattc ccccaccgcc gccggacttg ccaggtagcg tttcacctct ttcaccaact
    52321 gtgccgcgac cacttcggcg ctgacgcgct tttcaccgac gacaaaaaag cgttcggtga
    52381 tattttcact ttcgacttca agcgaaacgg tattacccgg tccctggtca cgcggcaggt
    52441 tatgaatatt ctgctcatgc agggaaaaac tccccgccag tgtagcgatt tcacgctcag
    52501 cgacatggcg cggcacgcca gctaatagca cctctccacg catctgcaca atgttcccgc
    52561 gctcgccaag ttgcaaggtg ttaaacaatg ccaccggcga gacttccgtt gccaccaccc
    52621 cgcctccagc aggataaaaa ccgtggcgta ataatgtggt ttgctgatga atgcccattt
    52681 tcgccagcag cggctccagc acccggcgga taaaatccgc aggcggtgcc gacgggttat
    52741 cggtgccgcc gctcacttca acacgcgaag gcccatcagc aaaccacagc gcgggtagca
    52801 ccgtttgcag caccagcgtg ctgcttccgg cactaccgat agcaaagcgg taatcgccgc
    52861 cacgcacggt accgggccgg aagaccagac gctgtgaccc cagctccgcg ccttccaccg
    52921 ttgccctaca aatttccgca gccgctttta ccgcggtcag atgttggcgc aacagccccg
    52981 gtttcgcccg cccggcacga atgccggtga tggtaaatgg ctggccggtt atcatcgaca
    53041 ggctcagcgc cgagcgcagg atctgcccgc cgccttcgcc ctgtgcgcca tccagcgcaa
    53101 tcatcctttt catcatttat ccttttacgc acaccacctg acgcagggta tagataactt
    53161 ccaccagatc gctttgtgcc gccatcaccg catcaatatc tttatacgcc atcgggattt
    53221 cgtcgatcac ttcggcatct ttacggcatt ccacatgcgc ggtggcacga atttgatctt
    53281 ccacgctgaa catttttttc gctttagtac ggctcataac ccgcccggca ccgtggctgc
    53341 acgaacagaa cgactcttca tttcccagcc cacggacgat aaagcttttc gctcccatcg
    53401 accctggaat aattccatat tgaccagcac gcgcagacac cgcgccttta cgcgtcacgt
    53461 agatctcttc accaaagtgc tgttcttttt gcacatagtt gtggtgacag ttgatctctt
    53521 ccatcgccag cgtttgtggc tgtctgaccg ttttctgcgt aatgctctgc aacgccgtta
    53581 ccacgttttc catcatcgca tcgcggttaa ggctggcaaa aagctgcgcc caggccacgg
    53641 ctttcaggta atcatcaaag tattccgtac cttccataaa gtacgccaga tcacgcgacg
    53701 gcaatgtttc aagcgtttcc tgcatctctt tttgcgccag atcgataaag taagtcccga
    53761 tggcgttacc aattccgcgt gaaccggagt gcagcataat ccacacctgg tccgactcat
    53821 caaggcagat ttcaataaag tggttacccg ttcccagcgt tcccaggtgt ttatagttgt
    53881 tagtgttaag gaaacgggga tatttttgcg ttaaccactg ataaccggct tccagctcag
    53941 cccatttcgc atcgacgtta acaggcggat tttcccatgc gcctttatca cgtttacaac
    54001 gtccggtagt acgtccgtgc ggcacggctg tttcaatcgc ctgacgcagc tccgccaggt
    54061 tttccggcag gtctgccgcc gttaacgcgg tacgcagcgc gttcattcca caaccgatat
    54121 ccacgcccac cgccgccgga ataatcgccc ctttggtcgg gatcacgcta ccaatggtgg
    54181 aacctttacc caggtgtaca tccggcatta ccgcaatatg tttgaaaata aacggcatct
    54241 tcgccgtatt aataagttgc tgacgcgcat cggcctctac cggcacgcct ttggtccaca
    54301 tttttaccgg ggcattttca gtggtcagta attcgtaatt cattttgttt ttctcttttc
    54361 gttgtttgct gtcctgataa ttgcaaccgt cgtgccagaa aattaaataa gcagcttaat
    54421 ttttttattt attgttttta aaaagattat atctttacgt ccgtaaccgg agatttcccg
    54481 caaagccgat ttaccgataa tgaaatctcg tcttttataa ggatatctaa gatgcgtaaa
    54541 acagtggctt ttggctttgt cggaacggtg ctggattatg ccgggcgcgg cagtcagcgc
    54601 tggtcgaaat ggcgtccgtc actctgtcta tgccagcaag aatcgttagt catcaatcga
    54661 ctggaattgt tgcacgacgc ccgctcgcgc tcgctgtttg aaacgcttaa atgcgatatc
    54721 gccagcgttt cgccagaaac tgaagtggtg agcgttgaga ttgaactgca taacccgtgg
    54781 gatttcgaag aggtttacgc ctgcctgcat gatttcgccc gtggctacgc gtttcaacct
    54841 gataaagaag actatttaat tcacatcacc actggcaccc acgtcgcaca gatttgctgg
    54901 tttctgctgg cggaagcgcg ttacctgccc gcccggctga tacaatcttc cccaccgcgc
    54961 aaaaaagaac agccgcgcgg cgcaggtgaa gtgacgatta tcgatctcga tttaagccgt
    55021 tataacgcca tcgccagccg ctttgccgag gaacggcagc aaacgctcga ttttcttaag
    55081 tccggcattg ccacgcgtaa cccccacttc aaccgcatga ttgagcagat tgaaaaagtg
    55141 gcgattaaat cccgcgcacc gatcctgctt aacggcccga caggtgcggg caagtcattt
    55201 ctggcacgac gcatcttcga attaaaacag gcacggcatc agtttagcgg cgcatttgta
    55261 gaaatgaact gcgccactct gcgtggcgat accgccatgt cgacgctatt tggtcatgtg
    55321 aaaggcgcgt ttaccggggc gcgggagtcg cgggaaggat tattacgcag cgccaacggc
    55381 gggatgttat ttcttgatga gattggcgaa ctgggcgcag acgaacaggc aatgctgctg
    55441 aaagccattg aagagaaaac cttttatccg tttggcagcg atcgccaggt gagtagcgac
    55501 tttcagctta tcgccggaac ggtgcgcgat ttgcgtcagc tggttgccga aggcaaattc
    55561 cgcgaagatc tgtacgcgcg gatcaatctc tggaccttca ccttgccggg gctgagccag
    55621 cgccaggaag atattgaacc gaaccttgat tatgaagtgg agcgccacgc ctcactcact
    55681 ggcgacagcg tgcgttttaa caccgaagcg cggcgcgcct ggctggcttt tgcgacctct
    55741 ccccaggcaa catggcgcgg taactttcgc gaactttctg ccagcgtcac gcggatggca
    55801 acctttgcca ctagcggacg catcactcgg gaagtggtgg aagatgagat aaaccgtctg
    55861 cgctataact ggcaggagag tcgcccctcc gcgattacgg cgttgctggg cgcagaggca
    55921 gaaaacatcg atctcttcga ccgtatgcaa ctggaacacg taatagccat ctgccgccag
    55981 gcaaagtcac tttctgctgc cggtcgccag ctttttgatg tttcgcgcca gggcaaagcc
    56041 agcgtcaacg acgcagatcg gctacgcaaa tacctggcgc gttttggtct gacgtgggaa
    56101 gccgtgcagg atcagcacag ctccagttga atatgatggt ccgtcagcac ctgcatgacg
    56161 cttactggcg gcggggcgtc ggtgtagaca gcatcaacca tgctgatgct ccccatattg
    56221 accatcgcat tacggccaaa tttcgagtga tcgacaacca gcataacgtg gcgcgagttc
    56281 tcaataatgg cgcgtttggt gcgaacttcg tggtaatcga attccagcaa cgagccgtcg
    56341 ctatcgatgc cgctgatccc cagaatgccg aaatcaaggc ggaactggga gataaaatcg
    56401 agcgtcgctt cgcctatgat cccaccatcg cggctacgta attcgccacc ggcgagaata
    56461 atgcgaaaat cctcttttac catcagcgtg ttagcgacgt taagattgtt ggtgacaatg
    56521 cgcaaattgc tgtgattgag cagcgcgtgc gccaccgctt ccggggtggt gccgatatcg
    56581 ataaacagcg ttgagccatt aggaatttgc tccgccactt tgcgggcgat gcgctctttt
    56641 tcttcggtct gggtggcttt acgatcgtgc catggcgtgt taaccgaact ggaaggcagc
    56701 gccgcaccgc catgatggcg caggatcagg ttttgctctg ccagctcgtt gaggtcgcga
    56761 cgaatagtct gcgggctgac ggagaagtgc tccaccagtt cctcggtact gacataaccc
    56821 tgctgtttaa ccagttcgat aataccgttg tgacgttgtg tttgtttcat ttataaatcc
    56881 ctggaattat tttcgttttc gcgcattgag cgaatcaaca aaagccatcg ctaaacccac
    56941 ggctaacccg gcgatgtgtg ctccgttcgc catcgacatc ccaaacaaat caaaccatcc
    57001 ggcgacaatc cagatcagcg caaagataat taacccacgt tgcaggtaaa tgccactttg
    57061 cggatcgcgt tcgccacgta gccagacgta gcccatcagc gcatacacca cgccagaaag
    57121 cccgccaaac cacggcccgc tgaatttttg ctgcacatag ccgcttaaca gggcgctaat
    57181 gagagtaatg acaattagct taccgctacc gaggcgtttt tccaccgcac cgccgagata
    57241 ccaccaccag agcaggttaa agaggatatg catcagcgag aagtgcatta acgcgtgggt
    57301 gaagtaacgc cagaactcaa atttcagtgt tggatcgaat ggccaggcca gccataacat
    57361 cacttcctga tcgccgagaa tttgcatggc aataaacacc accacgcagg cgatcatcat
    57421 cacccaggtt accggacctg cgcgttcacg caaggcagca aagaaaggat aacggcgata
    57481 atgcaggcca ctgccggtat ggcccgcctg ccagctggcc gccagataac gcggatctgc
    57541 cgggttttcg agaaaacgcg ccagctctgc ccgcacgcgc tcggcctggg actcatccgc
    57601 cagccagaca tcgctttggt tatgttgttg aatcgtgagg ataacaccct gcgtcgccat
    57661 gtaatcaaca aacgcctgcg ccacgcgggg gttagcaaaa gaggtaatca tcaacatcgt
    57721 tgctgtcgct tattccacac aaaaggggac agtataaagc gttacgcgcc gtacgccacc
    57781 tctgcaggaa actgacgccg ccaggcttca aagccgccgt caatgctata gaccacatcg
    57841 tagccctgtt gcagcagata ctgcgccgcg cctttgctgc tattgccgtg ataacacatc
    57901 accatcaccg gtgtgtcaaa gtcgttatca cgcataaacg cgcccagtgt gtcgttagtt
    57961 aaatggaaag cctgcaccgc atgccccatt gcgaaactct gtggatcgcg aatatcgacc
    58021 agcaccgcct ctttttcctg caacttctgg tgcgcgacgg caacgttaat acattcgaac
    58081 tgctccatgc gtctctcttt ctttacaaac aagtgggcaa atttaccgca cagtttacgt
    58141 cgaagcggca gataaacgcc ataatgttat acatatcact ctaaaatgtt ttttcaatgt
    58201 tacctaaagc gcgattcttt gctaatatgt tcgataacga acatttatga gctttaacga
    58261 aagtgaatga gggcagcatg gaaaccaaag atctgattgt gataggtggc ggcatcaatg
    58321 gtgctggtat cgcggcagac gccgctggac gcggtttatc cgtgctgatg ctggaggcgc
    58381 aggatctcgc ttgcgcgacc tcttccgcca gttcaaaact cattcacggt ggcctgcgct
    58441 accttgagca ctatgaattc cgcctggtca gcgaggcgct ggctgaacgt gaagtgctac
    58501 tgaaaatggc cccgcatatc gccttcccga tgcgttttcg cctgccgcat cgtccgcatc
    58561 tgcgcccggc gtggatgatt cgcattggtc tgtttatgta cgatcatctg ggtaaacgca
    58621 ccagcctgcc gggatcaacc ggtttgcgtt ttggcgcaaa ttcagtgtta aaaccggaaa
    58681 ttaagcgcgg attcgaatat tccgactgtt gggtagacga cgcccgtctg gtactcgcca
    58741 acgcccagat ggtagtgcgt aaaggcggtg aagtgcttac tcgtactcgt gccacttctg
    58801 ctcgccgcga aaacggcctg tggattgtgg aagcggaaga tatcgatacc ggcaaaaaat
    58861 atagctggca agcgcgcggc ctcgtgaacg ccaccggccc gtgggtaaag cagttcttcg
    58921 acgacgggat gcatctgcct tcgccttatg gcattcgcct gatcaaaggc agccatattg
    58981 tcgtgccgcg cgttcataca caaaagcagg cctacattct gcaaaacgaa gataaacgta
    59041 ttgtcttcgt gatcccgtgg atggacgagt tttccatcat cggcaccacc gatgtggaat
    59101 acaaaggtga tccaaaagca gtgaagattg aagagagtga aatcaattac ctgctgaaag
    59161 tgtataacac gcactttaaa aagcagttaa gccgggacga tatcgtctgg acttactccg
    59221 gcgtgcgtcc gctgtgtgat gatgagtccg attcgccgca ggctatcact cgtgattaca
    59281 cccttgatat ccatgatgaa aacggcaaag cgccgctgct gtcggtattc ggcggtaagc
    59341 tgaccaccta ccgcaaactg gcggagcacg cgctggaaaa actgacgcca tattatcagg
    59401 gcattggtcc ggcgtggacg aaagagagcg tgctgccggg tggtgccatt gaaggcgacc
    59461 gcgacgatta tgccgctcgc ctgcgccgcc gctatccgtt cctgactgaa tcgctggcac
    59521 gccattacgc gcgcacgtac ggcagcaaca gcgagttgct gctcggcaat gcggggacgg
    59581 taagcgatct cggggaagat ttcggtcatg agttctacga agcggagctg aaatacctgg
    59641 tggatcacga atgggtccgc cgcgccgacg atgccctgtg gcgtcgcaca aaacaaggca
    59701 tgtggctgaa tgcggaacaa caatctcgtg tgagtcagtg gctggtggag tatacgcagc
    59761 agaagttgtc gctggcgtcg taaggatgat gcccggttgt gaagtgcaac cgggctattt
    59821 ggattcctta gactattcat tgtaaataat tgaataacgg cccctgagct aaaatcgcca
    59881 aacttaagca cgttccccac tcaacccact tctgcccctg gcttctattt ttgctacgcc
    59941 actggcagta atatcccaac gcaaaaccaa gctgtgcact aatcgggccg acaaaatcag
    60001 tccacggctg cggccaaaaa ggggaaaaat tcaggggaag ccagacaaac gtcagaaaga
    60061 agtttcggat attttccagc aacgctgatt tctctggagt aatataaaga atgaaattcg
    60121 agagaattaa ccagaaatag gccggagaaa agaccagcca gtaatgcagt ctgtttttta
    60181 attcaggagg cgttctgcgg gagaattgcc aaccgatgcc acccattaca ctgaaaaaaa
    60241 gtgcatattc aaccattaac tcaagttgta attcctgaag agaaatacgc acaaccagga
    60301 gcggaaagga gaatattatt tcagccagaa aagttaacgt gaatataagt acggctaagg
    60361 caataaccgt cggccatacc aataatggca cgataacaaa accgagaatt acagggggaa
    60421 tataaagccg gaatttaatc atttttattt tcctggtgat tcggatgatg cgtcatacat
    60481 cgcgcataaa cactcatcaa tcatccagtc gtcttcccag acctgataat gtgtgccctg
    60541 ttcgggctga ttaagcaggt tctcaatcgc ctgccgcgca cgttctttca ggatgaattg
    60601 attgttgaag gcccaggcat tgatttgggc ggtaatggct ttatcgtcat catcctgctc
    60661 aaaacgacta ttctgaatat tctcttccct gttatgccac agcaaacatg ccatcgatac
    60721 tgtgggttgg taaccgggta cagagatgat ggcatcaagc aatggcgaaa gagcgatatc
    60781 ggcacgggaa aacatttcca gcatatactg taattcgaca gcctcatcgc cagataccca
    60841 atcagccata tactggcagg caaatcgctg caatattgcc cgttcatcct cacgccagtt
    60901 ttctttactg gcaaggttga tacgccgtag agaaaatact tcgtgaatat tgtcaaattg
    60961 cccctgcgca acaaactcca gtaaacgagg aagaaaataa cgaacctcat cgctgttttg
    61021 cggatcaggt cctggactac tattccaggc attgattaag gaataaggaa tcgctcgcaa
    61081 cgatgtattc cgtaatgctt tttgttcctg ttgactcagg caatattcac aacaaaccac
    61141 aaattgctgc ggcaccggat aacgacgaaa aatatgataa acctgctcaa ttaatgcctg
    61201 catggcggag ctgggttgca taattacccc ctcttccgct ggtcgcgtgt aaacggttga
    61261 taaacaaaac gagcattcgc cgcattcagg cgaataatgg aactacgaga gagggaaaag
    61321 ctgggccgaa tgcaataatt acaatcccga cagctttcct ctgtgcaatt ttccactgag
    61381 taatgacgcg cgtagagttt cagttcagcc tgcttatccc gaaaaccgcg taagctgtta
    61441 tcggtatgga atttccatgc ttctttaaac acctgaaaga tataaacatc ctcaatccag
    61501 gagtcacatt ttttaagcgg gcgctttgct atgatcaccc gctcatccgg attgagcatt
    61561 gccgtaaaat gaacggtaaa gtcatttgtc gtattcaggt cacattgaac atctggtaca
    61621 tcactgatat aggggtcaaa tccaaaatca gcaccacagg cacaaagacg accgatcgtg
    61681 gcggtatcga gggaaaatcc cggccataca ttgctggagg ttaagccaat atttaatgtg
    61741 acatcatatc gagataatcc ctcaagaccg acagtaccgg cgaacttttc cccttcatcc
    61801 aggacctggt taatgagttg ctgaataatg ccttcatccc agtatgttga tttgcagttg
    61861 gcaatcgtaa tatccacgcc agtgaatgga tcagtaccgg agaggcttgc aaataacgta
    61921 tcaactaatt ccatttttat tatccggctt aatgcaacat tttatccatc ggcacataac
    61981 ctacattttc caccagattt tgtccctgta gaattaaaaa tatttttttg ttttacattc
    62041 tgttcatccc tattattgca ttttattttg cggtgatggc taaaccgatt ctgtactagc
    62101 tcaaaaatta ctgatattcc actcgcaata cagccagtgc attataacga cctgccgcag
    62161 gcacttttcc agttgtacta acaggtaaag ccaggaaagt aacatcagca gtttgcgcag
    62221 aagcagcctg gttttccacc aatctgaacg gaacgcctac tgatgttgcc agagcattta
    62281 aatctacgga gttttgctga ctatccataa tcataatacc aacatcagga ttatcttgtg
    62341 cctgaataat actttgtttc ccattggtat cacgtgtagc tgaaatgtta ccgtcgaata
    62401 ccaacgtcat taccgcatct gtatctttat tcgtacaatt aatcggaata gttgttgcta
    62461 caggagtaac accagcaggc ttcgctccag caccagcccg gctcaattca ctggcccaca
    62521 catccggcat attaacagta aaactagtac cctcattaat agtgcaacct tgcggaacct
    62581 gaatatccag gttactgatc caggcattaa ataatggtac tggcccatgt tccgtttgcc
    62641 gctgactggc atacaatgaa actaaaagta cattattcag agagacatca gagatgattt
    62701 ttttatccaa cctaatttta atatagcctt ctgttcctga agaccagtaa tctgctttac
    62761 ttctgtcagc aggataatta gtactatagt t
//
LOCUS       DAZFLD010000005        59835 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.005, whole genome shotgun sequence.
ACCESSION   DAZFLD010000005 DAZFLD010000000
VERSION     DAZFLD010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 59835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59835
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.005"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            253..4140
                     /gene="purL"
                     /locus_tag="ACHKBL_000259"
                     /gene_synonym="purI"
     CDS             253..4140
                     /gene="purL"
                     /locus_tag="ACHKBL_000259"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HFO5817185.1"
                     /translation="MMEILRGSPALSAFRINKLLARFQAARLPVHTIYAEYVHFADLN
                     APLNDDEHAQLERLLKYGPALASHAPQGKLLLVTPRPGTISPWSSKATDIAHNCGLQQ
                     VNRLERGVAYYVEAGTLTNEQWQQVTAELHDRMMETVFFALDDAEQLFAHHQPTPVTS
                     VDLLGQGRQALIDANLRLGLALAEDEIDYLQDAFTKLGRNPNDIELYMFAQANSEHCR
                     HKIFNADWVIDGEQQPKSLFKMIKNTFETTPDHVLSAYKDNAAVMEGSEVGRYFADHE
                     TGRYDFHQEPAHILMKVETHNHPTAISPWPGAATGSGGEIRDEGASGRGAKPKAGLVG
                     FSVSNLRIPGFEQPWEEDFGKPERIVTALDIMTEGPLGGAAFNNEFGRPALNGYFRTY
                     EEKVNSHNGEELRGYHKPIMLAGGIGNIRADHVQKGEINVGAKLVVLGGPAMNIGLGG
                     GAASSMASGQSDADLDFASVQRDNPEMERRCQEVIDRCWQLGDANPILFIHDVGAGGL
                     SNAMPELVSDGGRGGKFELRDILSDEPGMSPLEIWCNESQERYVLAVAADQLPLFDEL
                     CKRERAPYAVIGEATEELHLSLHDRHFDNQPIDLPLDVLLGKTPKMTREVQTLKAKGD
                     ALVREGITIADAVKRVLHLPTVAEKTFLVTIGDRSVTGMVARDQMVGPWQVPVANCAV
                     TTASLDSYYGEAMAIGERAPVALLDFAASARLAVGEALTNIAATQIGDIKRIKLSANW
                     MAAAGHPGEDAGLYEAVKAVGEELCPALGLTIPVGKDSMSMKTRWQEGNEEREMTSPL
                     SLVISAFARVEDVRHTITPQLSTEDNALLLIDLGKGNNALGATALAQVYRQLGDKPAD
                     VRDVAQLKGFYDAIQALVAQRKLLAYHDRSDGGLLVTLAEMAFAGHCGIDADIASLGD
                     DRLAALFNEELGAVIQVRAADREAVEAVLAQHGLADCVHYVGQAVSGDRFVITANGQT
                     VFSESRTTLRVWWAETTWQMQRLRDNPECADQEHQAKSNDADPGLNVKLSFDINEDVA
                     APYIATGARPKVAVLREQGVNSHVEMAAAFHRAGFDAIDVHMSDLLAGRTGLEDFHAL
                     VACGGFSYGDVLGAGEGWAKSILFNDRVRDEFATFFHRPQTLALGVCNGCQMMSNLRE
                     LIPGSELWPRFVRNTSDRFEARFSLVEVTQSPSLLLQGMVGSQMPIAVSHGEGRVEVR
                     DAAHLAALESKGLVALRYVDNFGKVTETYPANPNGSPNGITAVTTESGRVTIMMPHPE
                     RVFRTVSNSWHPENWGEDGPWMRIFRNARKQLG"
     gene            4697..6124
                     /gene="qseE"
                     /locus_tag="ACHKBL_000260"
     CDS             4697..6124
                     /gene="qseE"
                     /locus_tag="ACHKBL_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two component system sensor histidine kinase
                     QseE/GlrK"
                     /protein_id="HFO5817186.1"
                     /translation="MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQA
                     ALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHA
                     GVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSR
                     GQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLG
                     NSVSFSGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLA
                     DQVVGPLTPEQKEVVSILDSSSRNLQKLIEQLLDYNRKQADSAVELENVELAPLVETV
                     VSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSS
                     HGARVYIDVINTGTPIPQEERAMIFEPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQG
                     ELYLVDESGQDVCFRIDLPSSKNTK"
     gene            6289..7002
                     /gene="qseG"
                     /locus_tag="ACHKBL_000261"
     CDS             6289..7002
                     /gene="qseG"
                     /locus_tag="ACHKBL_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system QseEF-associated
                     lipoprotein QseG"
                     /protein_id="HFO5817187.1"
                     /translation="MRHIFQRLLPRRLWLAGLPCLALLGCVQSHNKPAIDTPAEEKIP
                     VYQLADYLSTECSDIWALQGKSTETNPLYWLRAMDCADRLMPAQSRQQARQYDDGNWQ
                     NTFKQGILLADAKITPYERRQLVARIDALSTEIPAQVRPLYQLWRDGQALQLQLAEER
                     QRYSKLQQSSDSELDTLRQQHHVLQQQLELTTRKLENLTDIERQLSTRKPAGNFSPDT
                     PHESEKPAPSTDEVTPDEP"
     gene            6992..8326
                     /gene="glrR"
                     /locus_tag="ACHKBL_000262"
     CDS             6992..8326
                     /gene="glrR"
                     /locus_tag="ACHKBL_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator GlrR"
                     /protein_id="HFO5817188.1"
                     /translation="MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGAEGLRV
                     LNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFS
                     FLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSV
                     LINGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVS
                     NREGLFQAAEGGTLFLDEIGDMPAPLQVKLLRVLQERKVRPLGSNRDIDIDVRIISAT
                     HRDLPKAMARGEFREDLYYRLNVVSLKIPALAERTEDIPLLANHLLRQAAERHKPFVR
                     AFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFV
                     EARNQFELNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE"
     gene            8387..8725
                     /gene="glnB"
                     /locus_tag="ACHKBL_000263"
     CDS             8387..8725
                     /gene="glnB"
                     /locus_tag="ACHKBL_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HFO5817189.1"
                     /translation="MKKIDAIIKPFKLDDVREALAEVGITGMTVTEVKGFGRQKGHTE
                     LYRGAEYMVDFLPKVKIEIVVPDDIVDTCVDTIIRTAQTGKIGDGKIFVFDVARVIRI
                     RTGEEDDAAI"
     gene            complement(8770..9960)
                     /gene="hmpA"
                     /locus_tag="ACHKBL_000264"
     CDS             complement(8770..9960)
                     /gene="hmpA"
                     /locus_tag="ACHKBL_000264"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008941 - nitric oxide dioxygenase NAD(P)H
                     activity [Evidence IEA];
                     GO_function: GO:0019825 - oxygen binding [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0051409 - response to nitrosative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HFO5817190.1"
                     /translation="MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFN
                     MSNQRNGDQREALFNAIAAYASNIENLPALLPAVEKIAQKHTSFQIKPEQYNIVGEHL
                     LATLDEMFSPGQEVLDAWGKAYGVLANVFINREAEIYNENASKAGGWEGTRDFRIVAK
                     TPRSALITSFELEPVDGGAVAEYRPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYR
                     IAVKREEGGQVSNWLHNHANVGDVVKLVAPAGDFFMAVADDTPVTLISAGVGQTPMLA
                     MLDTLAKAGHTAQVNWFHAAENGDVHAFADEVKELGLSLPRFTAHTWYRQPSEADRAK
                     GQFDSEGLMDLSKLEGAFSDPTMQFYLCGPVGFMQFAAKQLVDLGVKQENIHYECFGP
                     HKVL"
     gene            10289..11542
                     /gene="glyA"
                     /locus_tag="ACHKBL_000265"
     CDS             10289..11542
                     /gene="glyA"
                     /locus_tag="ACHKBL_000265"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HFO5817191.1"
                     /translation="MLKREMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQAN
                     FAVYTALLEPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIVPYGIDASGHIDYADLEK
                     QAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVP
                     HAHVVTTTTHKTLAGPRGGLILAKGGSEELYKKLNSAVFPGGQGGPLMHVIAGKAVAL
                     KEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEAD
                     AALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEAEAKELAGWMCDVLDS
                     INDEAVIERIKGKVLDICARYPVYA"
     gene            complement(11605..12798)
                     /locus_tag="ACHKBL_000266"
     CDS             complement(11605..12798)
                     /locus_tag="ACHKBL_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFO5817192.1"
                     /translation="MRACINNQQIRHHNKCVILELLYRQKRANKSTLARLAQISIPAV
                     SNILQELESEKRVVNIDDESQTRGHSSGTWLIAPEGDWTLCLNVTPTSIECQVANACL
                     SPKGEFEYLQIDAPTPQALLSEIEKCWHRHRKLWPDRTINLALAIHGQVDPVTGVSQT
                     MPQAPWTTPVEVKYLLEEKLGIRVMVDNDCVMLALAEKWQNNSQERDFCVINVDYGIG
                     SSFVINEQIYRGCLYGSGQIGHTIVNPDGVVCDCGRYGCLETVASLSALKKQARVWLK
                     SQPVNTQLDPEKLTTAQLIAAWQSGEPWITSWVDRSANAIGLSLYNFLNILNINQIWL
                     YGRSCAFGEHWLNTIIRQTGFNPFDRDEGPSVKATKIGFGQLSRAQQVLGIGYLYVEA
                     QLRQI"
     gene            12916..16197
                     /locus_tag="ACHKBL_000267"
     CDS             12916..16197
                     /locus_tag="ACHKBL_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
                     /protein_id="HFO5817193.1"
                     /translation="MTPVKVWQERVEIPTYETGPQDIHPMFLENRVYQGSSGAVYPYG
                     VTDTLSEQKTLKSWQAVWLENDYIKVMILPELGGRVHRAWDKVKQRDFVYHNEVIKPA
                     LVGLLGPWISGGIEFNWPQHHRPTTFMPVDFTLEAHEDGAQTVWVGETEPMHGLQVMT
                     GFTLRPDRAALEIASRVYNGNATPRHFLWWANPAVKGGEGHQSVFPPDVTAVFDHGKR
                     AVSAFPIATGTYYKVDYSAGVDISRYKNVPVPTSYMAEKSQYDFVGAWCHDEDGGLLH
                     VANHHIAPGKKQWSWGHSEFGQAWDKSLTDNNGPYIELMTGIFADNQPDFTWLDAYEE
                     KRFEQYFLPYHSLGMVQNASRDAVIKLQRSERGIEWGLYAISPLKGYRLAIREIGKCN
                     ALLDDAVALTPATAIQGVLHGINPERLTIELSDADGNITLSYQEHQPQELPLPDVAKA
                     PLAAQDITSTDEAWFIGQHLEQYHHASRSPFDYYLRGVALDPLDYRCNLALAMLEYNR
                     ADFPQAVAYATQALKRAHALNKNPQCGQASLIRASAYERQGQYQQAEEDFWRAVWSGN
                     SKAGGYYGLARLAARNGHFDAGLDFCQQSLRACPTNQEVLCLHNLLLVLSGRQDNARL
                     QREKLLRDYPLNATLWWLNWFDGRSESALAQWRGLCQGRDVNALMTAGQLINWGMPTL
                     AAEMLNALDCQRTLPLYLQASLLPKAERGELVTKAIDVFPQFVRFPNTLEEVAALESI
                     EECWFARHLLACFYYNKCSYNKAITLWQRCVEMSPEFADGWRGLAIHAWNKQHDYELA
                     ARYLDNAYQLAPQDARLLFERDLLDKLSGVTPEKRLARLENNLEIALKRDDMTAELLN
                     LWHLTGQADKAADILATRKFHPWEGGEGKITSQFILNQLLRAWQHLDAREPQQASELL
                     HAALHYPENLSEGRLPGQTDNDIWFWQAICANAQGDETEATRCLRLAATGDRTINIHS
                     YYNDQPVDYLFWQGMALRLLGEQHTAQQLFSEMKQWAQEMAKTSIEADFFAVSQPDLL
                     SLYGDLQQQHKEKCLMVAMLASAGLGEVAHYESARAELMAINPAWPKAALFTTVMPFI
                     FSYVH"
     gene            16294..17277
                     /locus_tag="ACHKBL_000268"
     CDS             16294..17277
                     /locus_tag="ACHKBL_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HFO5817194.1"
                     /translation="MPKKMTRTRNLLLMATLLGSALFARAADKEMTIGAIYLDTQGYY
                     AGVRQGVQDAAKDSSVQVQLIETNAQGDISKESTFVDTLVARNVDAIILSAVSENGSS
                     RTVRRASEAGIPVICYNTCINQKGVDKYVSAYLVGDPLEFGKKLGNAAADYFIANKID
                     QPKIAVINCEAFEVCVQRRKGFEEVLKSRVPGAQIVANQEGTVLDKAISVGEKLIIST
                     PDLNAIMGESGGATLGAVKAVRNQNQAGKIAVFGSDMTTEIAQELENNQVLKAVVDIS
                     GKKMGNAVFAQTLKVINKQADGEKVIQVPIDLYTKTEDGKQWLATHVDGLP"
     gene            17300..18811
                     /locus_tag="ACHKBL_000269"
     CDS             17300..18811
                     /locus_tag="ACHKBL_000269"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HFO5817195.1"
                     /translation="MFTATEAVPVAKVVAGNKRYPGVVALDNVNFTLNKGEVRALLGK
                     NGAGKSTLIRMLTGSERPDSGDIWIGETRLEGDEATLTRRAAELGVRAVYQELSLVEG
                     LTVAENLCLGQWPRRNGMIDYLQMAQDAQRCLQALGVDVSPEQLVSTLSPAQKQLVEI
                     ARVMKGEPRVVILDEPTSSLASAEVELVISAVKKMSALGVAVIYVSHRMEEIRRIASC
                     ATVMRDGQVAGDVMLENTSTHHIVSLMLGRDHVDIAPVSPQEIVDQAVLEVRALRHKP
                     KLEDISFTLRRGEVLGIAGLLGAGRSELLKAIVGLETYEQGEIVINGEKITRPDYGDM
                     LKRGIGYTPENRKDAGIIPWLGVDENTVLTNRQKISANGVLQWSTIRRLTEEVMQRMT
                     VKAASSETPIGTLSGGNQQKVVIGRWVYAASQILLLDEPTRGVDIEAKQQIYRIVREL
                     AAEGKSVVFISSEVEELPLVCDRILLLQHGTFSQEFHAPVNVDELMSAILSVH"
     gene            18836..19834
                     /locus_tag="ACHKBL_000270"
     CDS             18836..19834
                     /locus_tag="ACHKBL_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO5817196.1"
                     /translation="MSASSLLLPQGKSVSLKQFVSRHINEIGLLVVIAILYLVFSLNA
                     PGFISLNNQMNVLRDAATIGIAAWAMTLIIISGEIDVSVGPMVAFVSVCLAFLLQFEV
                     PLAVACLLVLLLGALMGTLAGVLRGVFNVPSFVATLGLWSALRGMGLFMTNALPVPID
                     ENEVLDWLGGQFLGVPVSALIMMVLFALFVFISRKTAFGRSVFAVGGNATAAQLCGIN
                     VRRVRILIFTLSGLLAAVTGILLAARLGSGNAGAANGLEFDVIAAVVVGGTALSGGRG
                     SLFGTLLGVLVITLIGNGLVLLGINSFFQQVVRGVIIVVAVLANILLTQRSSKAKR"
     gene            19867..20961
                     /locus_tag="ACHKBL_000271"
     CDS             19867..20961
                     /locus_tag="ACHKBL_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HFO5817197.1"
                     /translation="MKINRIVNEGFMKTMLAAYLPGNSTVDLREVAVPTPGINQVLIK
                     MKSSGICGSDVHYIYHQHRATAAAPDKPLYQGFINGHEPCGQIVAMGQGCRHFKEGDR
                     VLVYHISGCGFCPNCRRGFPISCTGEGKAAYGWQRDGGHAEYLLAEEKDLILLPDALS
                     YEDGAFISCGVGTAYEGILRGEVSGSDNVLVVGLGPVGMMAMMLAKGRGAKRIIGVDM
                     LPERLAMAKQLGVMDHGYLATTEGLPQIIAELTHGGADVALDCSGNAAGRLLALQSTA
                     DWGRVVYIGETGKVEFEVSADLMHHQRRIIGSWVTSLFHMEKCAHDLTDWKLWPRNAI
                     THRFSLEQAGDAYALMASGKCGKVVINFPD"
     gene            20973..21845
                     /locus_tag="ACHKBL_000272"
     CDS             20973..21845
                     /locus_tag="ACHKBL_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HFO5817198.1"
                     /translation="MTIYTLSHGSLKLGVSDQGGVIEGFWRDTTPLLRPGKKNGVATD
                     ASCFPLVPFANRVSGNRFVWQGREYQLQPNVEWDAHYLHGDGWLGQWQCVSRNDDILC
                     LVYEHRSGVYHYRVSQTFHLTADTLTVTLSVTNQGAETLPFGTGWHPYFPLSPQTRIQ
                     AQASGYWLEREQWLAGEFCEQLPQELDFNQLAPLPRQWVNNGFAGWNGQARIEQPQEG
                     YAIIIETTPPAPCYFIFVSDPAFDKGYAFDFFCLEPMSHAPDDHHRPEGGDLIALAPG
                     ESTTSEMSLRVALL"
     gene            complement(21994..22416)
                     /locus_tag="ACHKBL_000273"
     CDS             complement(21994..22416)
                     /locus_tag="ACHKBL_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HFO5817199.1"
                     /translation="MNSLRYFDFGAARPVLLLIARIAVVLIFIIFGFPKMMGFDGTVQ
                     YMASLGAPMPMLAAIIAVVMEVPAAILIVLGFFTRPLAVLFIFYTLGTAVIGHHYWDM
                     TGDAVGPNMINFWKNVSIAGAFLLLAITGPGAISLDRR"
     gene            complement(22513..23715)
                     /gene="hcaD"
                     /locus_tag="ACHKBL_000274"
     CDS             complement(22513..23715)
                     /gene="hcaD"
                     /locus_tag="ACHKBL_000274"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylpropionate dioxygenase ferredoxin
                     reductase subunit"
                     /protein_id="HFO5817200.1"
                     /translation="MKEKTIIIVGGGQAAAMAAALLRQQGFSGELHLFSDEQHLPYER
                     PPLSKSMLLEDSPQLQSVLPAHWWQENNVHLHSGVTIKTLGRDTQELVLANGESWHWD
                     QLFIATGAAARPLPLLDALGERCFTLRHAGDAARLREVLQPERSVVIVGAGTIGLELA
                     ASATQRGCKVTVIELAASVMGRNAPPPVQRYLLPRHQQAGVRILLNNAIEHVVDGEKV
                     ELTLQSGETLRADVVIYGIGISANDQLAREANLDTANGIVIDEACRTCDPAIFAGGDV
                     AITRIDNGALHRCESWENANNQAQIAAAAMLGLPLPRLPPPWFWSDQYSDNLQFIGDM
                     HGDDWICRGNPETQKAIWFNLQNGVLIGAVTLNQGREIRLIRKWIQSGKTFDAKLLID
                     EDIALKSL"
     gene            complement(23725..24537)
                     /gene="hcaB"
                     /locus_tag="ACHKBL_000275"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
     CDS             complement(23725..24537)
                     /gene="hcaB"
                     /locus_tag="ACHKBL_000275"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
                     /EC_number="1.3.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate-dihydrodiol/cinnamic
                     acid-dihydrodiol dehydrogenase"
                     /protein_id="HFO5817201.1"
                     /translation="MSDLHNESIFITGGGSGLGLALAERFIEEGAQVATLELSAAKVA
                     SLRQRFGEHILAVEGNVTCYADYQRAVNQILTRSGKLDCFIGNAGIWDHNASLVNTPA
                     ETLETGFHELFNVNVLGYLLGAKACAPALIASEGSMIFTLSNAAWYPGGGGPLYTASK
                     HAATGLIRQLAYELAPKVRVNGVGPCGMASDLRGPQALGQSETSIMQSLTPEKIAAIL
                     PLQFFPQPADFTGPYVMLASRRNNRALSGVMINADAGLAIRGIRHVAAGLDL"
     gene            complement(24534..24854)
                     /locus_tag="ACHKBL_000276"
     CDS             complement(24534..24854)
                     /locus_tag="ACHKBL_000276"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phenylpropionate/cinnamic acid
                     dioxygenase ferredoxin subunit"
                     /protein_id="HFO5817202.1"
                     /translation="MNRIYACPVADVPEGEALRIDTSPVIALFNVGGEFYAINDRCSH
                     GNASMSEGYLEDDATVECPLHAASFCLKTGKALCLPATDPLTTYPVHVEGGDIFIDLP
                     EAQP"
     gene            complement(24854..25372)
                     /gene="hcaF"
                     /locus_tag="ACHKBL_000277"
     CDS             complement(24854..25372)
                     /gene="hcaF"
                     /locus_tag="ACHKBL_000277"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008695 - 3-phenylpropionate dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0019380 - 3-phenylpropionate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit beta"
                     /protein_id="HFO5817203.1"
                     /translation="MSAQVSLELHHRISQFLFHEASLLDDWKFRDWLAQLDEEIRYTM
                     RTTVNAQTRDRRKGVQPPTTWIFNDTKDQLERRIARLETGMAWAEEPPSRTRHLISNC
                     QVSETDIPNVFAVRVNYLLYRAQKERDETFYVGTRFDKVRRLEDGNWRLLERDIVLDQ
                     AVISSHNLSVLF"
     gene            complement(25369..26730)
                     /gene="hcaE"
                     /locus_tag="ACHKBL_000278"
     CDS             complement(25369..26730)
                     /gene="hcaE"
                     /locus_tag="ACHKBL_000278"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008695 - 3-phenylpropionate dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0019380 - 3-phenylpropionate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit alpha"
                     /protein_id="HFO5817204.1"
                     /translation="MTTPSDLNIYQLIDTQNGRVTPRIYTDPDIYQLELERIFGRCWL
                     FLAHESQIPKHGDFFNTYMGEDAVVVVRQKDGSIKAFLNQCRHRAMRVSYADCGNTRA
                     FTCPYHGWSYGINGELIDVPLEPRAYPQGLCKSHWGLNEVPCVESYKGLIFGNWDTSA
                     PGLRDYLGDIAWYLDGMLDRREGGTEIVGGVQKWVINCNWKFPAEQFASDQYHALFSH
                     ASAVQVLGAKDDGSDKRLGDGQTARPVWETAKDALQFGQDGHGSGFFFTEKPDANVWV
                     DGAVSSYYRETYAEAEQRLGEVRALRLAGHNNIFPTLSWLNGTATLRVWHPRGPDQVE
                     VWAFCITDKAASDEVKAAFENSATRAFGPAGFLEQDDSENWCEIQKLLKGHRARNSKL
                     CLEMGLGQEKRRDDGIPGITNYIFSETAARGMYQRWADLLSSESWQEVLDKTAAYQQE
                     VMK"
     gene            26866..27756
                     /gene="hcaR"
                     /locus_tag="ACHKBL_000279"
     CDS             26866..27756
                     /gene="hcaR"
                     /locus_tag="ACHKBL_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HcaR"
                     /protein_id="HFO5817205.1"
                     /translation="MELRHLRYFVAVAQALNFTRAAEKLHTSQPSLSSQIRDLENCVG
                     VPLLVRDKRKVALTAAGECFLQDALAILEQAENAKLRARKIVQEDRQLTIGFVPSAEV
                     NLLPKVLPMFRLRQPDTLIELVSLITTEQEEKIRRGELDVGLMRHPVYSPEIDYLELF
                     DEPLVVVLPVDHPLAHEKEITAAQLDGVNFVSTDPAYSGSLAPIVKAWFAQENSQPNI
                     VQVATNILVTMNLVGMGLGVTLIPGYMNNFNTGQVVFRPIAGNVPAIALLMAWKKGEM
                     KPALRDFIAIVQERLASVTA"
     gene            27916..29055
                     /locus_tag="ACHKBL_000280"
     CDS             27916..29055
                     /locus_tag="ACHKBL_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HFO5817206.1"
                     /translation="MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLL
                     LGAGLVARFLGSLLIAPRVSDPSRLISALRVLALLTLLFAVAFWAGAHVAWLMLVMIG
                     FNLFFSPLVPLTDALANTWQKQFPLDYGKVRLWGSVAFVIGSSLTGKLVTMFDYRVIL
                     ALLTLGVASMLLGFLIRPTIQPQGASRQQESTGWSAWLALVRQNWRFLACVCLLQGAH
                     AAYYGFSAIYWQAAGYSASAVGYLWSLGVVAEVIIFALSNKLFRRCSARDMLLISAIC
                     GVVRWGIMGATTALPWLIVVQILHCGTFTVCHLAAMRYIAARQGSEVIRLQAVYSAVA
                     MGGSIAIMTVFAGFLYQYLGHGVFWVMALVALPAMFLRPKVVPSC"
     gene            complement(29047..30327)
                     /gene="csiE"
                     /locus_tag="ACHKBL_000281"
     CDS             complement(29047..30327)
                     /gene="csiE"
                     /locus_tag="ACHKBL_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase inducible protein CsiE"
                     /protein_id="HFO5817207.1"
                     /translation="MMPTLAPPSVLSAPQRRCQILLTLFQPGLTATTATFSELNGVDD
                     DIASLDISETGREILRYHQLTLTTGYDGSYRVEGTVLNQRLCLFHWLRRGFRLCPSFI
                     TSHFTPALKSELKRRGIARNFYDDTNLQALVNLCSRRLQKRFETRDIHFLCLYLQYCL
                     LQHHAGITPQFNPLQRRWAESCLEFQVAQEIGRHWQRRALQPVPPDEPLFMALLFSML
                     RVPDPLRDAHQRDRQLRKSIKRLVNHFRELGNVRFYDEQGLCDQLYTHLAQALNRSLF
                     AIGIDNTLPEEFARLYPRLVRTTRAALAGFESEYGVHLSDEESGLVAVIFGAWLMQEN
                     DLHEKQIILLTGNDSEREAQIEQQLRELTLLPLNIKHMSVKAFLQTGAPRSAALIIAP
                     YTMPLPLFSPPLIYTDLTLTTHQQEQIRKMLESA"
     gene            complement(30518..31372)
                     /locus_tag="ACHKBL_000282"
     CDS             complement(30518..31372)
                     /locus_tag="ACHKBL_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417029.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFO5817208.1"
                     /translation="MALPVNKRVLKILFILFVVAFCIYLIPRIAINFFYYPDDKIYGP
                     DPWSAESVEFTAKDGTRLHGWFIPSATGPAEKAIATIIHAHGNAGNMSAHWPLVSWLP
                     ERNFNVFMFDYRGFGKSKGTPSQAGLLDDTQSAINVVRHRSDVNPQRLVLFGQSIGGA
                     NILDVIGQGDREGIRAVILDSTFASYATIANQMIPGSGYLLDESYSGENYIASVSPIP
                     LLLIHGKADHVIPWQHSEKLYSLAKEPKRLILIPDGEHIDAFSDRHGDVYREQMVDFI
                     LSALNPQN"
     gene            complement(31517..32320)
                     /gene="suhB"
                     /locus_tag="ACHKBL_000283"
     CDS             complement(31517..32320)
                     /gene="suhB"
                     /locus_tag="ACHKBL_000283"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0046854 - phosphatidylinositol phosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HFO5817209.1"
                     /translation="MHPMLNIAVRAARKAGNLIAKNYETPDAVEASQKGSNDFVTNVD
                     KAAEAVIIDTIRKSYPQHTIITEESGELEGTDQDVQWVIDPLDGTTNFIKRLPHFAVS
                     IAVRIKGRTEVAVVYDPMRNELFTATRGQGAQLNGYRLRGSTARDLDGTILATGFPFK
                     AKQYATTYINIVGKLFNECADFRRTGSAALDLAYVAAGRVDGFFEIGLRPWDFAAGEL
                     LVREAGGIVSDFTGGHNYMLTGNIVAGNPRVVKAMLANMRDELSDALKR"
     gene            32439..33179
                     /gene="trmJ"
                     /locus_tag="ACHKBL_000284"
     CDS             32439..33179
                     /gene="trmJ"
                     /locus_tag="ACHKBL_000284"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HFO5817210.1"
                     /translation="MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDS
                     QAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVA
                     EAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW
                     LATQENGEQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTR
                     ARPESQELNILRGILASIEQQNKGNKAE"
     gene            33449..33937
                     /gene="iscR"
                     /locus_tag="ACHKBL_000285"
     CDS             33449..33937
                     /gene="iscR"
                     /locus_tag="ACHKBL_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HFO5817211.1"
                     /translation="MRLTSKGRYAVTAMLDVALNSEAGPVPLADISERQGISLSYLEQ
                     LFSRLRKNGLVSSVRGPGGGYLLGKDASSIAVGEVISAVDESVDATRCQGKGGCQGGD
                     KCLTHALWRDLSDRLTGFLNNITLGELVNNQEVLDVSGRQHTHDAPRTRTQDAIDVKL
                     RA"
     gene            34049..35263
                     /gene="iscS"
                     /locus_tag="ACHKBL_000286"
     CDS             34049..35263
                     /gene="iscS"
                     /locus_tag="ACHKBL_000286"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017459011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HFO5817212.1"
                     /translation="MKLPIYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFG
                     WQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKT
                     EHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVV
                     QDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYV
                     RRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLRGLRNR
                     LWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLAVSSGSACTSASLE
                     PSYVLRALGLNDELAHSSIRFSLGRFTTEEEIDYTIELVRKSIGRLRDLSPLWEMYKQ
                     GVDLNSIEWAHH"
     gene            35291..35677
                     /gene="iscU"
                     /locus_tag="ACHKBL_000287"
     CDS             35291..35677
                     /gene="iscU"
                     /locus_tag="ACHKBL_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HFO5817213.1"
                     /translation="MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQ
                     IKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLDEAQAIKNTDIAEELELPPV
                     KIHCSILAEDAIKAAIADYKSKREAK"
     gene            35694..36017
                     /gene="iscA"
                     /locus_tag="ACHKBL_000288"
     CDS             35694..36017
                     /gene="iscA"
                     /locus_tag="ACHKBL_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HFO5817214.1"
                     /translation="MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEF
                     VDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKEGLNEGFKFTNPNVKDECGCG
                     ESFHV"
     gene            36113..36628
                     /gene="hscB"
                     /locus_tag="ACHKBL_000289"
     CDS             36113..36628
                     /gene="hscB"
                     /locus_tag="ACHKBL_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0097428 - protein maturation by iron-sulfur
                     cluster transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HFO5817215.1"
                     /translation="MDYFTLFGLPACYQLDTQALSLRFQDLQRQYHPDKFASGSQAEQ
                     LAAVQQSATINQAWQTLRHPLMRAEYLLSLHGFDLASEQHTVRDTAFLMEQLELREEL
                     DEIEQAKDEARLESFIKRVKKMFDTRHQLMVEQLDNETWDAAADTVRKLRFLDKLRSS
                     AEQLEEKLLDF"
     gene            36645..38495
                     /gene="hscA"
                     /locus_tag="ACHKBL_000290"
     CDS             36645..38495
                     /gene="hscA"
                     /locus_tag="ACHKBL_000290"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HFO5817216.1"
                     /translation="MALLQISEPGLSAAPHQRRLAAGIDLGTTNSLVATVRSGQAETL
                     ADHEGRHLLPSVVHYQQQGHSVGYDARTNAALDTANTISSVKRLMGRSLADIQQRYPH
                     LPYQFQASENGLPMIETAAGLLNPVRVSADILKALAARATEALAGELDGVVITVPAYF
                     DDAQRQGTKDAARLAGLHVLRLLNEPTAAAIAYGLDSGQEGVIAVYDLGGGTFDISIL
                     RLSRGVFEVLATGGDSALGGDDFDHLLADYIREQAGIPDRSDNRVQRELLDAAIAAKI
                     ALSDADSVTVNVAGWQGEISREQFNELIAPLVKRTLLACRRALKDAGVEADEVLEVVM
                     VGGSTRVPLVRERVGEFFGRPPLTSIDPDKVVAIGAAIQADILVGNKPDSEMLLLDVI
                     PLSLGLETMGGLVEKVIPRNTTIPVARAQDFTTFKDGQTAMSIHVMQGERELVQDCRS
                     LARFALRGIPALPAGGAHIRVTFQVDADGLLSVTAMEKSTGVEASIQVKPSYGLTDSE
                     IASMIKDSMSYAEQDVKARMLAEQKVEAARVLESLHGALAADAALLSTAERQVIDDAA
                     AHLSEVAQGDDVDAIEQAIKNVDKQTQDFAARRMDQSVRRALKGHSVDEV"
     gene            38497..38832
                     /gene="fdx"
                     /locus_tag="ACHKBL_000291"
     CDS             38497..38832
                     /gene="fdx"
                     /locus_tag="ACHKBL_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HFO5817217.1"
                     /translation="MPKIVILPHQDLCPDGAVLEANSGETILDVALRNGIEIEHACEK
                     SCACTTCHCIVREGFDSLPESSEQEDDMLDKAWGLEPESRLSCQARVTDEDLVVEIPR
                     YTINHAREH"
     gene            38844..39044
                     /gene="iscX"
                     /locus_tag="ACHKBL_000292"
     CDS             38844..39044
                     /gene="iscX"
                     /locus_tag="ACHKBL_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HFO5817218.1"
                     /translation="MGLKWTDSREIGEALYDAYPDLDPKTVRFTDMHQWICDLEDFDD
                     DPQASNEKILEAILLVWLDEAE"
     gene            39103..40386
                     /gene="pepB"
                     /locus_tag="ACHKBL_000293"
     CDS             39103..40386
                     /gene="pepB"
                     /locus_tag="ACHKBL_000293"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417018.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HFO5817219.1"
                     /translation="MTEAMKITLSTQPADARWGEKATYSINNDGITLHLNGTDDLGLI
                     QRAARKIDGLGIKHVQLSGEDWDADRCWAFWQGYKAPKGTRKVEWPDLDDAQRQELDN
                     RLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVAGDRVTYRITKGEDLRDQGYMGL
                     HTVGRGSERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSD
                     MGGAATVTGALAFAITRGLNKRVKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNT
                     DAEGRLVLADGLIDASAQKPELIIDAATLTGAAKTALGNDYHALFSFDDALAARLLAS
                     AAQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGW
                     LHIDCSATYRKAPVEQWSAGATGLGVRTIANLLTA"
     gene            40505..41281
                     /gene="sseB"
                     /locus_tag="ACHKBL_000294"
     CDS             40505..41281
                     /gene="sseB"
                     /locus_tag="ACHKBL_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB"
                     /protein_id="HFO5817220.1"
                     /translation="MSETKNELEDLLEKAATEPAHRPAFFRTLLESTVWVPGTAAQGE
                     AVVEDSALDLQHWEKEDGTSVIPFFTSLEALQQAVEDEQAFVVMPVRTLFEMTLGETL
                     FLNAKLPTGKEFMPREISLLIGEEGNPLSSQEVLEGGESLILSEVAEPPAQMIDSLTT
                     LFKTIKPVKRAFICSIKENEEAQPNLLIGIEADGDIEEIIQAAGSVATDTLPGDEPID
                     ICQVKKGEKGISHFITEHIAPFYERRWGGFLRDFKQNRII"
     gene            complement(41313..41438)
                     /gene="timP"
                     /locus_tag="ACHKBL_000295"
     CDS             complement(41313..41438)
                     /gene="timP"
                     /locus_tag="ACHKBL_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small toxic inner membrane protein TimP"
                     /protein_id="HFO5817221.1"
                     /translation="MKIRCFCIVLIVSGALLSEVNNNRSLSGNNLLAVNNLQSSK"
     gene            complement(42100..42945)
                     /gene="sseA"
                     /locus_tag="ACHKBL_000296"
     CDS             complement(42100..42945)
                     /gene="sseA"
                     /locus_tag="ACHKBL_000296"
                     /EC_number="2.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate sulfurtransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
                     /protein_id="HFO5817222.1"
                     /translation="MSTTWFVGADWLAEHIDDPEIQIIDARMASPGQEDRNVAQEYLN
                     GHIPGAVFFDIEALSDHTSPLPHMLPRPETFAVAMRELGVNQDKHLIVYDEGNLFSAP
                     RAWWMLRTFGVEKVSILGGGLAGWQRDDLLLEEGAVELPEGEFNAAFNPEAVVKVTDV
                     LLASHENTAQIIDARPAARFNAEVDEPRPGLRRGHIPGALNVPWTELVREGELKTTDE
                     LDAIFFGRGVSYDKPIIVSCGSGVTAAVVLLALATLDVPNVKLYDGAWSEWGARADLP
                     VEPEK"
     gene            43152..48113
                     /gene="yfhM"
                     /locus_tag="ACHKBL_000297"
     CDS             43152..48113
                     /gene="yfhM"
                     /locus_tag="ACHKBL_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha2-macroglobulin"
                     /protein_id="HFO5817223.1"
                     /translation="MKKLRVAACMLMLALAGCDNNDNAPTAVKKDAPSEVTKAASSEN
                     VSSAKLSAPERQKLAQQSAGKALTLLDLSEVQLDGAATLVLTFSIPLDPDQEFSRVIH
                     VVDKKSGKVDGAWELSDNLKELRLRHLEPKRDLIVTIGKEVKALNNATFSKDYEKTIT
                     TRDIQPSVGFASRGSLLPGKVIEGLPVMALNVNNVDVNFFRVKPESLPAFISQWEYRN
                     SLANWQSDKLLQMADLVYTGRFDLNSARNTREKLLLPLGDIKPLQQAGVYLAVMNQAG
                     RYDYSNPATLFTLSDIGVSAHRYHNRLDIFTQSLENGAAQQGVEVSLLNEKGQTLTQA
                     TSDAQGHVQLENDKNAALLLARKDGQTTLLDLKLPALDLAEFNIAGAPGYSKQFFMFG
                     PRDLYRPGETVILNGLLRDADGKALPDQPIKLDVIKPDGQVLRSVVSQPENGLYHFTW
                     PLDSNAATGMWHIRANTGDNQYRMWDFHVEDFMPERMALNLTGEKTPLTPKDEVRFSV
                     VGYYLYGAPANGNTLQGQLFLRPLREAVSALPGFEFGDIAAENLSRTLDEVQLTLDDK
                     GRGEVSTESQWKETHSPLQVIFQGSLLESGGRPVTRRAEQAIWPADALPGIRPQFASK
                     SVYDYRTDSTVKQPIVDEGSNAAFDIVYSDAQGVKKAVSGLQVRLIRERRDYYWNWSE
                     DEGWQSQFDQKDLIENEQTLDLKADETGKVSFPVEWGAYRLEVKAPNEAVSSVRFWAG
                     YSWQDNSDGSGAVRPDRVTLKLDKASYRPGDTIKLHIAAPTAGKGYAMVESSEGPLWW
                     QEIDVPAQGLDLTIPVDKTWNRHDLYLSTLVVRPGDKSRSATPKRAVGVLHLPLGDEN
                     RRLDLALETPTKMRPNQPLTVKIKASNKNGEMPKQVNVLVSAVDSGVLNITDYVTPDP
                     WQAFFGQKRYGADIYDIYGQVIEGQGRLAALRFGGDGDELKRGGKPPVNHVNIVAQQA
                     LPVTLNEQGEGSVTLPIGDFNGELRVMAQAWTADDFGSNESKVIVAAPVIAELNMPRF
                     MASGDTSRLTLDITNLTDKPQKLNVALTASGLLELVSDSPAAVELAPGVRTTLFIPVR
                     ALPGYGDGDIQATISGLALPGETVADQHKQWKIGVRPAFPAQTVNYGTALQPGETWAL
                     PADGLQNFSPVTLEGQLLLSGKPPLNIARYIKELKAYPYGCLEQTTSGLFPSLYTNAA
                     QLQALGIKGDSDEKRRASVDIGISRLLQMQRDNGGFTLWDKNGDEEYWLTAYVMDFLV
                     RAGEQGYSVPTDAINRGNERLLRYLQDPGMMSIPYADNLKASKFAVQSYAALVLARQQ
                     KAPLGALREIWEHRADAASGLPLLQLGVALKTMGDAMRGEEAIVLALKTPRNSDERIW
                     LGDYGSPLRDSALMLSLLEENKLLPDEQYSLLNTLSQQAFGERWLSTQESNALFLAAR
                     TLQDLPGKWQAQTTFSAEPLTGEKAQTSNLNSDQLATLQVTNSGDQPLWLRVDASGYP
                     QSAPLSASNVLQIERHILGTDGKSKSLDSLRSGDLVLVWLQVKASNSVPDALVVDLLP
                     AGLELENQNLANGSASLEQSGGEVQNLLNQMQQASIKHIEFRDDRFVAAVAVDEYQPV
                     TLVYLARAVTPGTYQVPQPMVESMYVPQWRATGAADDLLIVRP"
     gene            48114..50426
                     /gene="pbpC"
                     /locus_tag="ACHKBL_000298"
     CDS             48114..50426
                     /gene="pbpC"
                     /locus_tag="ACHKBL_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417014.1"
                     /note="penicillin-binding protein 1C; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase PbpC"
                     /protein_id="HFO5817224.1"
                     /translation="MPRLLTKRGCWIMLAAAPFIIILAAWAADKLWPLPLQEVNPARV
                     VVAQDGTPLWRFADADGIWRYPVTIEDVSPRYLEALINYEDRWFWMHPGVNPFSVARA
                     AWQDLTSGRVISGGSTLTMQVARLLDPHPKTFGGKIRQLWRALQLEWHLSKREILTLY
                     LNRAPFGGTLQGIGAASWAYLGKSPANLSYSEAAMLAVLPQAPSRIRPDRWPERAEAA
                     RNKVLERMAVQGVWSRERVKESREEPIWLAPRQMPQLALLFSRMMLGKSKSDKIVTTL
                     DAGLQRRLEELAQNWKGRLPPRSSLAMIVVDHTDMRVRGWVGSVDLNDDSRFGHVDMV
                     NAIRSPGSVLKPFVYGLALDEGLIHPASLLQDVPRRTGDYRPGNFDSGFHGPISMSEA
                     LVRSLNLPAVQVLEAYGPKRFAAKLRNVGLPLYLPNGAAPNLSLILGGAGAKLEDMAA
                     AYTAFARHGKAGKLRLQPDDPLLERPLMSSGAAWIIRRIMADEAQPLPDGALPRVAPL
                     AWKTGTSYGYRDAWAIGVNARYVIGIWTGRPDGTPVVGQFGFASAIPLLNQVNNILLS
                     RSVNLPEDPRPDSVSRGVICWPGGQSLAEGDGNCRRRLATWLLDGSQPPTLLLPEQEG
                     INGIRFPIWLDENGKRVAADCPQARQEMINVWPLPLEPWLPASERRAVRLPPASTICP
                     PYGHDAQLPLQLTGVRDGAIIKRLPGAAEATLPLQSSGGAGERWWFLNGEPLTERGRN
                     VTLHLTDKGDYQLLVMDEVGQIATVKFVMQ"
     gene            50575..51006
                     /gene="ndk"
                     /locus_tag="ACHKBL_000299"
     CDS             50575..51006
                     /gene="ndk"
                     /locus_tag="ACHKBL_000299"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HFO5817225.1"
                     /translation="MAIERTFSIIKPNAVAKNVIGSIFARFEAAGFKIVGTKMLHLTV
                     EQARGFYAEHDGKPFFDGLVEFMTSGPIVVSVLEGENAVQRHRDLLGATNPANALAGT
                     LRADYADSLTENGTHGSDSVESAAREIAYFFGEGEVCPRTR"
     gene            51156..52310
                     /locus_tag="ACHKBL_000300"
     CDS             51156..52310
                     /locus_tag="ACHKBL_000300"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HFO5817226.1"
                     /translation="MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKPFRADQ
                     VMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQ
                     RVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
                     AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPA
                     LDKLGDMIDVALAISLHAPNDEIRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVT
                     IEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPGAPYGRSSNSRIDRFSKVL
                     MSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV"
     gene            52595..53602
                     /gene="rodZ"
                     /locus_tag="ACHKBL_000301"
     CDS             52595..53602
                     /gene="rodZ"
                     /locus_tag="ACHKBL_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HFO5817227.1"
                     /translation="MNTEATHDQNEALTTGARLRNAREQLGLSQQAVAERLCLKVSTV
                     RDIEEDKAPADLASTFLRGYIRSYARLVHIPEEELLPGLEKQAPLRAAKVAPMQSFSL
                     GKRRKKRDGWLMTFTWLVLFVVIGLSGAWWWQDHKAQQEEITTMADQSSAELNNNQSQ
                     SVPLDTSTTTDQAMATTPTSPVDTTATNTQTPAVTAPAPAVDPQQNAVVPPSQANVDT
                     AATPAPAATTTPDGAAPLPTDQAGVTTPAVDPNALVMNFTADCWLEVTDATGKKLFSG
                     MQRKDGNLNLTGQAPYKLKIGAPAAVQIQYQGKPVDLSRFIRTNQVARLTLNAEQSPA
                     Q"
     gene            53629..54747
                     /gene="ispG"
                     /locus_tag="ACHKBL_000302"
                     /gene_synonym="gcpE"
     CDS             53629..54747
                     /gene="ispG"
                     /locus_tag="ACHKBL_000302"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity (ferredoxin) [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HFO5817228.1"
                     /translation="MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEAT
                     VNQIKALERVGADIVRVSVPTMDAAEAFKLIKQQVNVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
                     AMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAV
                     KSAIGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGV
                     RKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVEK"
     gene            54858..56132
                     /gene="hisS"
                     /locus_tag="ACHKBL_000303"
     CDS             54858..56132
                     /gene="hisS"
                     /locus_tag="ACHKBL_000303"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HFO5817229.1"
                     /translation="MAKNIQAIRGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLP
                     IVEQTPLFKRAIGEVTDVVEKEMYTFEDRNGDSLTLRPEGTAGCVRAGIEHGLLYNQE
                     QRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWWRTLGISEH
                     VTLELNSIGSLEARANYRDALVAFLEQHKEKLDEDCKRRMYTNPLRVLDSKNPEVQVL
                     LNDAPALGDYLDEESREHFAGLCKLLESAGIAYTVNQRLVRGLDYYNRTVFEWVTNSL
                     GSQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNPEFKADPVVDIYLV
                     ASGADTQSAAMALAERLRDELPGVKLMTNHGGGNFKKQFARADKWGARVAVVLGESEV
                     ANGTAVVKDLRSGEQTAVAQDSVAAHLRTLLG"
     gene            56150..56770
                     /gene="yfgM"
                     /locus_tag="ACHKBL_000304"
     CDS             56150..56770
                     /gene="yfgM"
                     /locus_tag="ACHKBL_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ancillary SecYEG translocon subunit YfgM"
                     /protein_id="HFO5817230.1"
                     /translation="MEIYENENDQVEAVKRFFAENGKALAVGVILGVGALIGWRYWNS
                     HQVDSARSASLAYQNAVTAVSEGKPDSIPAAEKFAAENKNTYGALASLELAQQFVDKN
                     ELEKAAAQLQQGLADTSDENLKAVINLRLARVQVQLKQADAALKTLDAIKGEGWAAIV
                     ADLRGEALLSKGDKQGARSAWEAGVKSDVTPALSEMMQMKINNLSI"
     gene            56781..57959
                     /gene="bamB"
                     /locus_tag="ACHKBL_000305"
     CDS             56781..57959
                     /gene="bamB"
                     /locus_tag="ACHKBL_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HFO5817231.1"
                     /translation="MQLRKLLLPGLLSVTLLSGCSLFNSEEDVVKMSPLPTVENQFTP
                     TTAWSTSVGSGIGNFYSNLHPALADNVVYAADRAGLVKALNADDGKEIWSVNLAEKDG
                     WFSKDPALLSGGVTVSGGHVYIGSEKAQVYALNTSDGTVAWQTKVAGEALSRPVVSDG
                     LVLIHTSNGQLQALNEADGAVKWTVNLDMPSLSLRGESAPATAFGAAVVGGDNGRVSA
                     VLMEQGQMIWQQRISQATGSTEIDRLSDVDTTPVVVNGVVFALAYNGNLTALDLRSGQ
                     IMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWTQSDLLHRLLTSPVLYN
                     GNLVVGDSEGYLHWINVEDGRFVAQQKVDSSGFQTEPVAADGKLLIQAKDGTVYSITR
                     "
     gene            58077..59549
                     /gene="der"
                     /locus_tag="ACHKBL_000306"
     CDS             58077..59549
                     /gene="der"
                     /locus_tag="ACHKBL_000306"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HFO5817232.1"
                     /translation="MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGR
                     AEIEGREFICIDTGGIDGTEDGVETRMAEQSLLAIEEADVVLFMVDARAGLMPADEAI
                     AKHLRSREKPTFLVANKTDGLDPDQAVVDFYALGLGEIYPIAASHGRGVLSLLEHVLL
                     PWMEDLAPQEEVDEDAEYWAQFEAEENGEEEEADDFDPQSLPIKLAIVGRPNVGKSTL
                     TNRILGEERVVVYDMPGTTRDSIYIPMERDGREYVLIDTAGVRKRGKITDAVEKFSVI
                     KTLQAIEDANVVMLVIDAREGISDQDLSLLGFILNSGRSLVIVVNKWDGLSQEVKEQV
                     KETLDFRLGFIDFARVHFISALHGSGVGNLFESVREAYDSSTRRVGTSMLTRIMTMAV
                     EDHQPPLVRGRRVKLKYAHAGGYNPPIVVIHGNQVKDLPDSYKRYLMNYFRKSLDVMG
                     SPIRIQFKEGENPYANKRNTLTPTQMRKRKRLMKHIKKSK"
     gene            59612..59827
                     /locus_tag="ACHKBL_000307"
     CDS             59612..59827
                     /locus_tag="ACHKBL_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001544841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HFO5817233.1"
                     /translation="MALNCPTCKTPLERNGETAHCATCDKDFTVQALCPECHQALQVL
                     KACGAVDYFCQHGHGLISKKRVEFIIA"
BASE COUNT        14131 a        15040 c        16652 g        14012 t
ORIGIN      
        1 aattctctgt gtcgtgcgcg tcccagcttg aaaaaacgta ataatagtga aaggtttact
       61 cataaatgag cggcattttg cgtaaacctg cgccagttgg caacttatta cagccattgg
      121 cggcacgcgt tgctaattca cgatggtgat tttatttcca cgcaaacggt ttcgtcagcg
      181 catcagattc tttataatga cgcccgtttc cccccccttg ggtacaccga aagcttagaa
      241 gacgagagac ttatgatgga aattctgcgt ggttcgcctg cattgtcggc attccgaatc
      301 aacaaactgc tggcacgttt tcaggctgcc aggctcccgg ttcacactat ttacgccgag
      361 tatgtccatt ttgctgacct caatgcgccg ttaaacgatg atgagcacgc acaacttgaa
      421 cgcctgctga aatatggccc ggcactcgcc agccacgccc cgcaaggcaa actcctgctg
      481 gtgaccccgc gtcctggcac catctctccc tggtcttcga aagcgaccga tatcgcccat
      541 aattgcggtc tacaacaggt aaaccgcctt gagcgcggcg ttgcttacta tgtagaagcc
      601 ggtacgctga ccaatgaaca atggcagcag gttaccgctg aactgcacga ccgcatgatg
      661 gaaacggtct tttttgcttt agatgatgca gagcagctgt ttgcacacca tcaaccgact
      721 ccggtcacca gcgttgattt gctggggcag ggccgtcagg cgctaatcga cgctaacctg
      781 cgtcttggcc tggcactggc ggaagatgaa attgactatt tgcaggatgc tttcacaaag
      841 ctgggtcgta acccgaacga catcgaactg tatatgtttg cccaggcgaa ctccgagcac
      901 tgccgccaca aaatttttaa cgccgactgg gttatcgatg gtgaacagca gccgaaatca
      961 ctgtttaaga tgatcaaaaa caccttcgaa accacgccgg atcacgttct ctctgcttat
     1021 aaagataacg ccgccgtaat ggaaggttct gaagtgggcc gctactttgc tgaccacgaa
     1081 acgggccgct acgatttcca tcaggaacct gcgcatatcc tgatgaaagt tgaaactcac
     1141 aaccacccga cggcgatttc tccgtggccg ggcgcggcga ccggttccgg cggtgaaatc
     1201 cgcgatgaag gtgcctccgg gcgcggcgcg aagccgaaag ctggtctggt cggtttctcc
     1261 gtgtccaacc tgcgaattcc tggcttcgaa cagccgtggg aagaagattt cggtaaacct
     1321 gagcgcattg ttaccgcgct ggacatcatg accgaaggcc cgctgggcgg tgcggcgttt
     1381 aacaacgaat ttggtcgtcc ggcactgaat ggctacttcc gtacttatga agaaaaagtg
     1441 aacagccaca atggcgaaga gctgcgcggt tatcacaaac cgatcatgct ggcgggcggg
     1501 atcggcaaca ttcgtgccga tcacgtacaa aaaggcgaga tcaacgttgg tgcgaagctg
     1561 gtcgttctcg gcggcccggc aatgaacatc ggtcttggcg gcggcgcggc gtcttccatg
     1621 gcgtctggtc agtctgatgc cgacctcgac ttcgcttccg tacagcgcga caacccggag
     1681 atggagcgtc gctgccagga agtgatcgac cgttgctggc agcttggtga tgccaaccca
     1741 atcctgttta tccacgacgt tggcgcaggc ggtctttcta acgccatgcc ggaactggtg
     1801 agcgacggcg ggcgcggcgg taaattcgaa ctgcgcgata tcctgagcga cgaaccgggc
     1861 atgagcccgc tggaaatctg gtgtaacgaa tcccaggaac gctacgtgct ggcagttgct
     1921 gccgatcagt taccgctgtt tgacgaactg tgtaagcgtg agcgcgcgcc atacgcggtg
     1981 attggtgaag cgaccgaaga actgcatctt tctttgcacg atcgtcattt tgataatcag
     2041 ccgatcgatc tgccgctgga cgtactgctt ggtaaaacgc cgaagatgac ccgcgaagta
     2101 caaacgctga aagcgaaggg tgatgcgctg gttcgtgaag ggattaccat tgctgacgcg
     2161 gtgaaacgcg tgctgcacct gccgaccgtg gcggagaaaa ctttcctggt gaccattggt
     2221 gaccgcagcg taaccggcat ggtggcgcgc gatcagatgg tgggtccgtg gcaggtgccg
     2281 gtcgctaact gcgcggtcac taccgccagc ctcgatagct actacggtga agcgatggcg
     2341 attggcgagc gtgcgccggt tgcgctgctg gatttcgccg cttctgcccg tctggcggtc
     2401 ggtgaagcgt taaccaacat cgccgcaaca caaattggcg atatcaaacg cattaaactt
     2461 tccgccaact ggatggcggc ggcaggccac cctggtgaag atgcaggcct gtatgaagcc
     2521 gttaaagccg tgggtgaaga actttgtccg gcgctgggcc tgacgatccc ggtgggtaaa
     2581 gactctatgt cgatgaaaac ccgttggcag gaaggtaacg aagagcgcga aatgacgtcg
     2641 ccgctgtcgc tggtgatttc tgcatttgcc cgcgtggaag atgtacgtca caccatcacg
     2701 ccacagcttt ctaccgaaga taacgcactg ctgctgattg atttgggcaa aggcaataac
     2761 gcgctgggcg caacggcgct ggcgcaggtt tatcgtcagc ttggcgacaa accggcagat
     2821 gtacgcgatg tcgcgcaact gaaaggcttc tatgacgcga ttcaggcgct ggttgcacag
     2881 cgtaagctgc tggcgtatca cgaccgctct gatggcggcc tgctggtaac gctggcggaa
     2941 atggcctttg ctggtcattg cggcattgac gcggatatcg cctctctggg tgacgatcgc
     3001 ctggcggcgt tgtttaacga agaactgggt gcggtgattc aggttcgtgc cgctgaccgt
     3061 gaagccgttg aagcggtact ggcacagcat gggcttgcgg attgcgtcca ttatgtaggg
     3121 caggcggttt ccggtgaccg ttttgtgatt accgccaacg ggcagactgt attcagcgaa
     3181 agccgcacta cgttgcgtgt ctggtgggca gaaactacct ggcagatgca gcgcctgcgt
     3241 gacaacccgg agtgtgccga tcaggagcac caggcgaaat caaacgacgc cgatccgggc
     3301 ctgaatgtga aactgtcgtt cgatatcaac gaagatgtgg cagcaccgta tattgccact
     3361 ggtgcgcgtc cgaaagttgc cgtgctgcgt gagcaggggg tgaactcgca tgttgaaatg
     3421 gcggcagctt tccaccgtgc aggcttcgat gcaatcgatg tgcatatgag cgacctgctg
     3481 gctggacgca cgggcctgga ggatttccac gctctggtag cgtgcggtgg attctcctac
     3541 ggtgacgtgc tgggtgctgg tgaaggctgg gcgaagtcta tcctgttcaa tgaccgtgta
     3601 cgcgatgagt ttgcaacctt cttccaccgt ccgcaaacgc tggcgctggg ggtatgtaac
     3661 ggttgccaga tgatgtctaa cctacgcgaa ctgatcccag gtagtgagtt gtggccacgt
     3721 tttgtgcgca atacctccga tcgctttgaa gcgcgtttca gcctggttga agtaacccaa
     3781 agcccgtctc tgctgttgca ggggatggtg ggctcgcaaa tgccgattgc tgtctctcat
     3841 ggcgaagggc gcgtggaagt gcgtgatgcg gcgcatctgg cggcactgga aagcaaaggg
     3901 ctggtggcac tgcgctatgt cgataacttc ggcaaagtca ctgaaaccta cccggctaac
     3961 ccgaacggtt ctccgaatgg tattacggca gtcacgactg aaagtggtcg agtcaccatt
     4021 atgatgccgc acccggaacg tgttttccgt actgtcagca actcctggca tccggaaaac
     4081 tggggcgagg atgggccgtg gatgcgtatt ttccgcaatg cacgtaagca gttggggtaa
     4141 gccgtcaacc cattggtttt gcagccggat gaggcattaa gcaccaacta ctattataag
     4201 ctcccaactt cgggagcttt tttgtgtctg taaatcacga caatgggtgg tttaccgcgt
     4261 cgctttctgg cgacacttaa ctaattgatt ttaatggcat ctaataagtt tatcttaagt
     4321 tgtctctttt tagcgacaca gtggctgatt tacagccaaa tagcgaagaa gccataaccc
     4381 cttcaaaaca gcgtacaatc agtacgttac caaactattt tctttattgg cacagttact
     4441 gcataatagt aaccagtggc tcattcaccg acttatgtca gccccttcgg gacgtgctac
     4501 ataaaatacg aatgacgcac aacaaggtgc ctgccgtcca acttctgata tcagcgtagc
     4561 tatatcaacc atcgggcgaa acgtcgagtt aggcaccgcc ttattccata acaaagccgg
     4621 gtaattcccg gctttgttgt atctgaactt cccctcggtt agcatcaggc tattcgcgtc
     4681 tgacgagagt aacaccttga aacgctggcc cgtttttccc cgctcattac gacaactggt
     4741 aatgctggca tttttgctga ttctgctgcc cctgttggtg ctggcatggc aagcctggca
     4801 aagcctgaat gcgcttagcg atcaggcggc gctggttaac cgcactacgc ttattgatgc
     4861 ccggcgtagt gaagcgatga ccaacgcggc gctggagatg gagcgtagct accgtcagta
     4921 ttgcgtgctg gacgacccta cgcttgcgaa ggtttatcaa agccagcgca agcgttacag
     4981 cgaaatgctc gatgcccacg caggcgtact gccagacgat aaactttacc aggcattgcg
     5041 tcaggatttg aacaatctgg cccaacttca gtgtaacaac agtggtcccg atgctgccgc
     5101 cgccgcgcgt cttgaagcct ttgccagtgc caataccgaa atggtgcagg ccacgcgcac
     5161 agtggtgttc tctcgtgggc agcaacttca gcgtgaaatc gccgaacgtg ggcaatattt
     5221 tggttggcaa tcgctggtgc tatttctggt gagtctggtg atggtgctac ttttcacgcg
     5281 gatgattatc gggccggtga aaaatatcga gcgcatgatc aaccgtctgg gggaagggcg
     5341 ttctctgggc aatagcgttt cgttcagtgg accgagcgag ttacgctcgg ttgggcaacg
     5401 tattctttgg ttaagtgagc gcctgtcatg gctggaatcc caacgccatc aatttttaag
     5461 acatttatct catgaattaa aaacgccact ggcgagtatg cgcgagggca ctgaattact
     5521 ggctgaccag gttgtcgggc cgcttacgcc agagcaaaaa gaggtggtga gcattcttga
     5581 tagcagcagc cgcaatttgc aaaaactgat cgaacaactg cttgattaca accgtaaaca
     5641 ggcggacagt gcggtggaac tggagaatgt tgaattagca ccgctggtgg agacagtggt
     5701 ttctgctcat agcctgcccg cacgggctaa aatgatgcat accgacgtcg atctcaaagc
     5761 aacagcttgc ctggcggagc caatgctgct gatgagcgta ctggataatc tttactccaa
     5821 tgcggtgcac tacggggctg aatccggtaa catttgcctt cgcagcagtt cacatggtgc
     5881 gcgggtttat attgatgtca tcaatacagg cacgcccatt ccgcaagagg aacgcgccat
     5941 gatcttcgaa cccttttttc agggaagcca ccagcgaaaa ggggcggtga agggcagcgg
     6001 tctgggatta agcattgcca gggattgtat tcgccgtatg caaggggaac tgtatctggt
     6061 cgacgagagc gggcaagacg tttgtttccg cattgattta ccgtcgtcga aaaacacgaa
     6121 ataatgctaa cagcccatcc catcaggcta ttttatttgt cagttcggcc ccgggcagtg
     6181 ctcgaaatct tcacgtacga catgtacgct ctggtttctc cgcgctgtcc atgtccaaac
     6241 tgcctgcaac aattacgccc actgggatag gctttaagtc tggtgaatat gcgacacatt
     6301 tttcaacgat tattgccccg acggctgtgg ctggcagggc tgccatgtct ggcattgctg
     6361 ggttgcgtgc agagtcacaa taagccagcc attgatacgc cagcagaaga aaaaattccg
     6421 gtttatcaac tggctgacta tctttctact gaatgtagcg acatctgggc gctgcaaggt
     6481 aaatcaaccg aaaccaatcc gctttactgg ctgcgggcga tggattgtgc tgatcgttta
     6541 atgcctgcgc agtcgcgtca gcaagcccgc caatatgacg acggcaactg gcaaaatacc
     6601 tttaaacaag ggattttgct cgccgacgcc aaaattacgc cgtatgagcg ccgccagctg
     6661 gtcgcgcgca ttgatgcgct aagtaccgag atcccggcac aggttcgccc tctgtatcaa
     6721 ctctggcgcg acggtcaggc gctgcaactg caactggcgg aagagcgcca acgctacagt
     6781 aaactccagc agtccagcga cagcgagctg gatactttgc gccagcaaca tcacgttcta
     6841 caacagcaac tggaactcac tacccgcaag ctggaaaacc tgaccgatat tgaacgccag
     6901 ctctcgaccc gcaaaccggc tggaaatttc tcgccggata cgccgcacga gagtgaaaag
     6961 cccgcgccat ccactgatga ggtcactcct gatgagccat aaacctgcgc atttattact
     7021 ggtcgatgac gatccgggat tgctgaaact gcttggcctg cgcctgacca gcgaaggcta
     7081 cagcgtggtc acggcggaaa gtggtgctga aggattacgg gtactgaatc gcgaaaaagt
     7141 agatttagtt atcagcgacc tgcggatgga tgaaatggat ggtatgcagc tgtttgctga
     7201 aatccagaaa gtgcagccgg gaatgccggt aattattctt accgcacatg gttctattcc
     7261 cgatgccgtt gctgcaacac agcagggagt gttcagtttc ctcaccaagc ctgtcgataa
     7321 agacgcgcta tatcaggcaa ttgacgatgc gctggaacaa tccgcgccag ccaccgatga
     7381 acgctggcgc gaggcgattg tcacccgcag cccgctgatg ctgcgtttgc tggaacaggc
     7441 gcggctggtg gcgcaatcag acgttagcgt tttgattaac ggtcagagcg gcaccgggaa
     7501 agagattttc gcccaggcta tccacaacgc cagcccgcgc aatagcaaac catttattgc
     7561 tattaactgt ggtgcattgc ccgagcaatt gctggagtcg gagctgtttg gtcatgcgcg
     7621 tggcgcgttt actggcgctg tcagcaatcg cgaaggttta ttccaggcgg cggaaggcgg
     7681 tacgctattt ctcgatgaga ttggcgatat gcctgcaccg ttgcaggtca aactgctgcg
     7741 tgtgttgcag gagcgtaaag tgcgcccgct gggcagtaac cgcgatattg atatcgacgt
     7801 gcggattatt tccgccactc accgcgacct gccaaaagcg atggcgcgcg gggaattccg
     7861 tgaagatctc tattaccgcc tcaacgttgt cagcctgaaa attccggctc tggcagagcg
     7921 cacagaagac attccgctac tggcaaatca cctgttgcgc caggcggcag agcgacataa
     7981 accgtttgtc cgcgcgttct ccaccgatgc gatgaaacgc ctgatgaccg ctagctggcc
     8041 gggtaatgta cgccagttgg tcaacgtgat tgaacagtgc gtggcgctga cctcatctcc
     8101 ggtgattagc gatgcattag tagagcaggc gctggagggc gaaaacacgg cgctgccaac
     8161 ctttgttgag gcacgtaatc agtttgaact caactatttg cgtaagctgc tgcaaatcac
     8221 caaaggcaac gtcacccacg cggcgagaat ggcggggcgc aaccggacag aattttataa
     8281 actgctttcc cgacacgagc tggatgcaaa cgatttcaag gaatgaattg gcgttatgtg
     8341 ttacgtttag cagatcaaaa gacaggcgac cttttcaagg aatagcatga aaaagattga
     8401 tgcgattatt aaacccttca agctggacga tgttcgcgaa gcactggctg aagtcggcat
     8461 taccggcatg acggtcactg aggtgaaagg ttttggccgc cagaaaggcc ataccgagct
     8521 gtaccgcggc gcggagtata tggtggattt tctgccgaaa gtgaaaattg agattgtcgt
     8581 accggacgac attgtcgata cctgtgtcga taccattatt cgcacggcgc aaaccggcaa
     8641 aatcggtgac ggtaaaatct tcgtctttga cgtggcacgg gtcattcgta tccgtaccgg
     8701 tgaagaagac gacgcggcaa tttaaaccgc gtcaagggtt gccggatgtt tccatccggc
     8761 aacatcaaat cacagcacct tatgcgggcc aaagcattcg taatgaatat tttcctgctt
     8821 cacgcccaga tccactaact gtttcgcggc aaactgcatg aagccaaccg ggccgcagag
     8881 atagaactgc attgtcggat cgctgaacgc accttccagt ttgctcaaat ccatcagacc
     8941 ttcgctatca aactgacctt tagcgcgatc ggcttcgctc ggctgacgat accaggtgtg
     9001 cgcggtaaag cgcggcagtg acagccccag ttccttaact tcatcagcaa aggcgtgaac
     9061 atcgccattt tctgccgcat ggaaccagtt cacttgtgct gtgtggcctg cttttgccag
     9121 tgtgtcgagc attgccagca ttggcgtttg accgacaccg gcagagatta acgtcactgg
     9181 tgtgtcatct gcgacagcca taaaaaaatc acctgctgga gcgaccagtt tcacgacatc
     9241 gccaacattg gcgtgattgt gcagccagtt ggatacctgc ccaccctctt cgcgtttcac
     9301 cgcaatacga tagcctttgc catccggttt gcgagtcaaa gagtactgac gaatttcctg
     9361 atgcgggaaa ccttccggct tcagccagac gccgagatat tgccccggac ggtattctgc
     9421 cactgcgcca ccgtcgaccg gctccagttc gaagctggtg ataagcgcgc tgcgcggtgt
     9481 tttagccaca atgcggaaat cgcgagtacc ttcccagcca ccggctttgc tggcgttttc
     9541 gttatagatt tccgcctcgc gattgataaa tacattagcc agtacgccat aggctttacc
     9601 ccacgcgtcc agcacttcct ggcccgggct gaacatttcg tccagcgtcg ccaacaggtg
     9661 ttcaccgaca atgttgtact gttccggttt gatctggaag ctggtgtgct tctgcgcgat
     9721 tttttctacc gctggcagca gcgcaggcag gttttcaata ttactggcgt aggcggcaat
     9781 agcgttaaac agggcttcac gttgatcgcc attgcgctgg ttactcatgt taaaaatttc
     9841 tttgagttct gggttatgag taaacatacg gtcgtagaaa tgggcggtta actttggtcc
     9901 cgtttccacc agtaaaggga tggtggcttt tactgtagcg atggtttgag cgtcaagcat
     9961 atggtcttcc ttttttttgc atcttaattg atgtatctca aatgcatctt ataaaaaata
    10021 gccctgcaat gtaaatggtt ctttggtgtt tttcagaaag aatgtgatga agtgaaaaat
    10081 ttgcatcaca aacctgaaaa gaaatccgtt tccggttgca agctctttat tctccaaagc
    10141 cttgcgtagc ctgaaggtaa tcgtttgcgt aaattccttt gtcaagacct gttatcacac
    10201 aatgattcgg ttatactgtt cgccgttgtc caacaggacc gcctataaag gccaaaaatt
    10261 ttattgttag ctgagtcagg agatgcggat gttaaagcgt gaaatgaaca ttgccgatta
    10321 tgatgccgaa ctgtggcagg ctatggagca ggaaaaagta cgtcaggaag agcacatcga
    10381 actgatcgcc tccgaaaact acaccagccc gcgcgtaatg caggcgcagg gttctcagct
    10441 gaccaacaaa tatgctgaag gttatccggg caaacgctac tacggcggtt gcgagtatgt
    10501 tgatatcgtt gaacaactgg cgatcgatcg tgcgaaagaa ctgttcggtg ctgactacgc
    10561 taacgtccag ccgcactccg gctcccaggc taactttgcg gtgtacaccg cgctgctgga
    10621 accaggtgat accgttctgg gtatgaacct ggcgcatggc ggtcacctga ctcacggttc
    10681 tccggttaac ttctccggta aactgtacaa catcgttcct tacggtatcg atgctagcgg
    10741 tcatatcgac tacgccgatc tggaaaaaca agccaaagaa cacaaaccga aaatgattat
    10801 cggtggcttc tctgcatatt ccggcgtggt ggactgggcg aaaatgcgtg aaatcgctga
    10861 cagcatcggt gcttacctgt tcgttgatat ggcgcacgtt gcgggcctgg ttgcagctgg
    10921 cgtctacccg aacccggttc ctcatgctca cgttgttact accaccactc acaaaaccct
    10981 ggcgggtccg cgcggcggcc tgatcctggc gaaaggtggt agcgaagagc tgtacaaaaa
    11041 actgaactct gcggttttcc ctggtggtca gggtggtccg ttgatgcacg taatcgccgg
    11101 taaagcggtt gctctgaaag aagcgatgga gcctgagttc aaaacttacc agcagcaggt
    11161 cgctaaaaac gctaaagcga tggtagaagt gttcctggaa cgcggctaca aagtggtttc
    11221 cggcggcact gataaccatc tgttcctggt tgatctggtt gataaaaacc tgaccggtaa
    11281 agaagctgac gccgctctgg gccgtgctaa catcaccgtc aacaaaaaca gcgtaccgaa
    11341 cgatccgaag agcccgtttg tgacctccgg tattcgcgtg ggtactccgg cgattacgcg
    11401 tcgcggcttc aaagaagcag aagcgaaaga actggctggc tggatgtgtg acgtgctgga
    11461 cagcatcaat gatgaagccg ttatcgagcg catcaaaggt aaagttctcg acatctgtgc
    11521 acgttacccg gtttacgcat aagcgaaacg gttaatctgt tgaaaaaggc ttccttcggg
    11581 aagcctttct acgttatcgc gccatcaaat ctgtcgtaac tgcgcctcaa catacaaata
    11641 gccaatcccc agcacctgtt gtgcgcggct taattgccca aagccaattt tcgtcgcttt
    11701 cacgctcggt ccttcgtcgc ggtcgaacgg gttaaatccc gtctggcgaa taatggtatt
    11761 aagccagtgc tcaccaaagg cacaactgcg accgtacaac caaatctgat taatattgag
    11821 gatgttgagg aagttataca gactcaaacc aatggcattg gcagagcgat caacccagct
    11881 ggtgatccac ggttctccac tctgccaggc agcgattaac tgcgctgttg tcagtttttc
    11941 aggatcaagc tgagtattaa ccggttgtga ttttagccat acccgcgcct gtttttttaa
    12001 tgcacttaac gaggcgacgg tttccaggca gccgtaacgt ccacagtcgc agacgacgcc
    12061 atccggatta acgatggtgt gaccaatctg tccgctacca tacaaacagc cgcgataaat
    12121 ttgctcgtta atcacgaacg acgagccaat gccgtaatca acgttgatca cgcagaaatc
    12181 ccgttcctgc gaattatttt gccatttctc cgccagcgcc agcatcacgc agtcattatc
    12241 gaccatcacc cgaatgccga gcttctcttc cagcagatac tttacctcaa ccggcgttgt
    12301 ccacggcgct tgcggcatgg tttgcgacac gccggtcacc ggatcaacct gaccgtggat
    12361 tgccagcgcc aggttgatgg tacggtccgg ccacaattta cggtggcgat gccagcattt
    12421 ttcgatttcg gacagcagcg cctgcggcgt cggtgcatca atctgtaaat actcaaattc
    12481 acctttcgga cttaaacaag cattagcaac ctgacactca atactggtgg gcgtcacgtt
    12541 caggcacaac gtccagtcac cttccggcgc aatcagccat gtaccgctac tatgcccgcg
    12601 cgtctggctt tcatcgtcga tattcaccac ccgtttttcg ctttccagtt cctgcaaaat
    12661 attactgacc gccggaatcg aaatttgcgc cagccgggcc agcgttgatt tattggcgcg
    12721 cttttgccgg tacagcagtt ccagaatcac gcatttgtta tggtggcgaa tctgttgatt
    12781 attaatgcag gctctcatct tcactaaacc ttgtttagtt aattgcgtga ttattccgcg
    12841 aaaacattgc cagattgttc aatacactgc cacaaatctt ttaattcagt atgtcttgtt
    12901 aatattgagg gcaccatgac tccagtaaaa gtgtggcaag agcgcgttga gatcccgacc
    12961 tatgaaaccg ggccgcagga tatacatccc atgttcctgg aaaatcgcgt ttatcaggga
    13021 tcgtccggcg cggtttatcc ctacggcgtg accgatacgc tgagcgagca gaaaaccctg
    13081 aaatcctggc aggcggtgtg gctggaaaac gactacatca aagtgatgat cctgccggag
    13141 ctgggcggtc gggtgcatcg cgcgtgggat aaagtgaaac agcgcgattt tgtctatcat
    13201 aatgaagtca ttaaaccggc gctggtgggg ttgctggggc cgtggatctc cggcgggatt
    13261 gagtttaact ggccgcaaca ccatcgcccg accaccttta tgcccgtcga tttcaccctc
    13321 gaagcccatg aagacggcgc acaaaccgtg tgggtcggcg aaacggagcc gatgcacggt
    13381 ttacaggtga tgacaggttt caccctgcgc cctgaccggg cggcactgga aattgccagc
    13441 cgcgtctata acggcaacgc cacgccgcgt catttcttgt ggtgggccaa cccggcagtg
    13501 aaagggggtg aagggcatca gagcgtcttc ccgccggatg taacggcggt gtttgatcac
    13561 ggcaaacggg ccgtctccgc tttccccatc gccaccggca cttactacaa agtggactac
    13621 tccgccggag tggacatttc tcgctataaa aatgtgcccg ttccaacctc atatatggct
    13681 gaaaaatcac agtacgattt tgttggcgcg tggtgtcacg atgaagatgg cggtttgctg
    13741 cacgttgcca accaccatat tgcgccaggt aaaaaacagt ggagctgggg acacagtgaa
    13801 tttggccagg cgtgggataa gagtctgacc gacaataacg gcccgtatat cgaactgatg
    13861 actggtattt ttgccgataa ccagcccgat tttacctggc ttgatgctta cgaagagaag
    13921 cgttttgagc agtatttcct gccttaccat tctttgggca tggtgcaaaa cgcctcccgc
    13981 gatgcggtga ttaaactcca gcgtagcgag cgggggattg agtgggggct gtatgccatc
    14041 tctccgttga aaggataccg tctggcgatc cgcgaaatcg gcaaatgcaa cgcgttgctc
    14101 gatgatgccg tggccctgac accagcgacc gccatccagg gcgtgttgca cggtatcaat
    14161 cctgaaaggc tgaccattga actctccgat gccgacggca atatcacact gagttatcag
    14221 gaacatcagc cgcaagagtt gccgttgccg gacgtcgcca aagcgccact ggcagcacaa
    14281 gacattacca gtacagatga agcctggttt atcggtcagc atctggagca atatcatcac
    14341 gccagccgtt caccgttcga ttactacctg cgcggcgtgg cgctggatcc gctggattat
    14401 cgctgtaacc tggcgctggc gatgctggaa tataaccgag cagatttccc gcaagcggtg
    14461 gcgtatgcca ctcaggcgct gaaacgcgca catgcgctaa acaaaaatcc gcagtgcgga
    14521 caggcgagtt tgattcgcgc cagtgcttac gaacgtcagg ggcaatatca acaagccgaa
    14581 gaggatttct ggcgggcggt ctggagcggc aacagtaaag caggtggcta ttatggtctg
    14641 gcacggctgg cggcgcgtaa tggtcacttc gacgctggtc tggatttttg ccaacaaagt
    14701 cttcgcgcct gcccaaccaa tcaggaagtg ctttgcctgc ataatctgct gctggtgtta
    14761 agtggtcgtc aggacaacgc gcgtttgcag cgcgagaaac tgctgcgtga ttatccgctg
    14821 aacgccactc tgtggtggct gaactggttc gatggtcgta gcgaatcagc cctcgcgcag
    14881 tggcgcggtc tgtgtcaggg acgcgacgtt aacgccctga tgaccgccgg gcaactgatt
    14941 aactggggaa tgcccaccct ggcggcagag atgctgaacg cactggactg ccagcgcacg
    15001 ctgccgcttt acttgcaagc cagcttgctg ccgaaagctg aacgcggcga actggtcaca
    15061 aaagccattg atgtcttccc acagtttgtc cgtttcccga atacgctgga agaagtggcg
    15121 gcgctggaga gtattgaaga gtgctggttt gcccgtcatt tactggcctg cttctactac
    15181 aacaagtgca gctacaacaa agccattact ttatggcaac gttgcgtaga gatgtcgccg
    15241 gagtttgccg acggctggcg cgggttagcg atccatgcgt ggaataagca acacgattat
    15301 gagctggccg cgcgttatct tgataatgct tatcagcttg cgccgcagga tgcacgtctg
    15361 cttttcgaac gggatttgct ggataagtta agcggtgtca caccggagaa acgactggcg
    15421 cgtctggaaa ataatctgga aattgcgctg aaacgcgacg acatgaccgc agaactgctc
    15481 aatttgtggc atctcacggg tcaggcagac aaagcggcgg acattctcgc cacgcgcaaa
    15541 ttccacccgt gggaaggcgg ggaagggaag atcaccagtc agtttatcct caaccagtta
    15601 ttacgcgcct ggcagcatct tgatgccaga gagccgcagc aggccagcga actgcttcat
    15661 gccgcgttgc attatccgga gaatttaagc gaaggccgtt taccggggca aactgacaac
    15721 gacatctggt tctggcaggc gatatgcgcc aacgcgcagg gcgatgaaac tgaagcgacg
    15781 cgttgtttac gtctggcggc gactggcgat cgcaccatta acattcacag ttattacaac
    15841 gatcagccgg ttgattatct cttctggcaa ggaatggcgc tgcgactgct gggcgaacaa
    15901 cacaccgcac agcaactgtt tagtgaaatg aaacagtggg cgcaagagat ggcgaaaacc
    15961 agtatcgagg cggatttctt tgctgtttca caacctgacc tgttgtcgct gtatggcgat
    16021 ttacaacagc agcataaaga aaaatgcctg atggtggcga tgctggcgtc cgcgggactc
    16081 ggggaggttg cgcactatga atctgctcgc gctgaattga tggcgattaa tccagcctgg
    16141 ccgaaagcgg cattattcac caccgtgatg ccttttattt ttagctacgt tcactaaacc
    16201 ttattaactg taatatttga gcggcacgcg ctgccgctca cgctcaaata attaaaataa
    16261 gccgttgtaa ataaaaaccc tacaggaggc actatgccta aaaaaatgac aagaacccgt
    16321 aatttattat taatggctac gctattgggt agcgcactat ttgccagggc tgcggataaa
    16381 gaaatgacta ttggcgcaat ataccttgat acccagggat attacgccgg agtgcgtcag
    16441 ggcgttcagg atgcggcaaa agattcttca gtacaggtac agttaattga aactaacgct
    16501 cagggcgata tttcgaaaga aagtaccttt gttgataccc tcgtggcgcg taatgtcgat
    16561 gccattattt tatcggcagt gtctgaaaat ggcagtagtc gtaccgttcg tcgcgccagt
    16621 gaagcgggta ttccggtgat ttgctacaac acctgtatta atcaaaaagg tgtcgataaa
    16681 tatgtctcgg cgtatctggt cggcgatccg ctggaatttg gtaaaaaact gggtaacgct
    16741 gccgccgatt attttattgc caataaaatt gaccagccga aaattgccgt catcaattgc
    16801 gaagcctttg aagtttgtgt gcagcgacgt aaaggatttg aagaggtatt aaaatcccgc
    16861 gttcccggcg cgcaaatagt cgctaatcag gaagggactg ttttagataa agcgatttcc
    16921 gttggtgaaa aactaattat ctccacgccg gatctcaacg ccattatggg ggaatcgggc
    16981 ggtgcgacac tcggcgcggt aaaagcggta cgtaatcaaa atcaggccgg aaaaattgct
    17041 gttttcggtt cggatatgac cactgaaatt gctcaggagc tggaaaacaa tcaggtgctg
    17101 aaagcggtgg tggatatttc cggtaagaaa atgggcaatg ctgttttcgc gcaaactttg
    17161 aaggttatca ataaacaagc cgacggtgaa aaagtgattc aggtgccaat cgatctctat
    17221 accaaaacgg aagacggtaa acagtggctg gcaacgcacg ttgatggtct gccctaatcg
    17281 ctgattcgca gaggtcccta tgttcacagc aacagaggca gtcccggtag caaaagtggt
    17341 ggcaggaaat aagcgttatc ccggcgtcgt tgcgctggat aacgttaact tcacgctcaa
    17401 taaaggcgaa gttcgtgcgc tgttaggcaa aaacggtgcg ggaaaatcga ccctcattcg
    17461 aatgcttacc ggtagcgaac gtccggatag cggtgatatc tggattggcg agacgcgact
    17521 ggaaggtgac gaagctacgc tgactcgccg tgccgctgaa ctgggggtac gcgcggttta
    17581 tcaggagtta agtctggtgg aagggctgac agtggcggaa aacctctgcc tcggtcagtg
    17641 gccccgccgc aacggcatga ttgattacct gcaaatggcg caggacgccc aacgttgctt
    17701 gcaggcgttg ggcgttgacg ttagtcctga acaacttgtt tcaacactaa gcccggcgca
    17761 aaagcagctg gtggaaattg cgcgggtgat gaagggcgag ccgcgcgtgg tcattcttga
    17821 tgaacccacc agctcgcttg ccagtgcgga ggttgaactg gtgatcagcg cggtgaaaaa
    17881 gatgtcggca ctgggcgtgg cggtgattta tgtcagccac cggatggaag aaattcgccg
    17941 cattgcctcc tgtgccaccg ttatgcgcga tggtcaggtg gcgggcgatg tgatgctcga
    18001 aaacacctcc acgcatcata ttgtgtcgct aatgctcggg cgcgatcacg ttgatattgc
    18061 gccggtttcc cctcaggaaa ttgtcgatca ggccgtgctg gaagtccgtg cgttacgcca
    18121 taagcccaag ctggaggata tcagctttac gctccgtcgt ggcgaagtac tcggcattgc
    18181 tggcctgctg ggggcagggc gcagtgaatt gttgaaagcc atagttgggc tggagacgta
    18241 tgaacagggc gaaattgtta tcaacggcga gaaaatcact cgccccgatt acggcgacat
    18301 gctgaaacgc ggcattggct atacgccaga aaaccgcaaa gatgcgggga tcattccctg
    18361 gctgggcgtt gacgaaaata cagtgctgac caatcggcaa aaaatcagcg ccaacggtgt
    18421 gctgcaatgg tccaccatcc gccgcctgac cgaagaggtg atgcagcgga tgacggtcaa
    18481 ggccgccagt agcgaaacgc ccatcggcac gctttctggc ggcaatcagc aaaaagtggt
    18541 gatcggtcgt tgggtctatg ccgccagcca gattttgttg ctcgacgagc caacgcgtgg
    18601 cgtcgatatc gaagccaaac agcagattta ccgtattgtc cgcgagctgg ctgccgaagg
    18661 aaaaagcgtg gtgtttatct ccagtgaagt ggaggagttg ccgctggtgt gcgaccgcat
    18721 tctgttgtta cagcacggta cgttctcgca ggagtttcac gctccggtca atgtggatga
    18781 gctgatgtcc gccattctgt ctgtgcactg atgaatttta ccgaggaacc tgaagatgtc
    18841 tgcttcgtca ttactattgc cgcagggcaa gagcgtctcg ctcaaacaat ttgtcagtcg
    18901 ccatattaat gagatcggtt tgctggtggt gatcgccatt ctttatctgg tcttctctct
    18961 gaacgcgcct ggctttatct cattgaataa ccagatgaac gtgctgcgcg atgccgccac
    19021 catcgggatc gccgcatggg cgatgacgct gattattatc tccggtgaaa ttgatgtcag
    19081 cgttggtccg atggtggctt ttgtctcggt gtgcctggca tttttgctgc aatttgaagt
    19141 tccgctggcg gtggcgtgtc tgctggtgtt gctgttaggc gcgctgatgg gaacgctcgc
    19201 cggggtgctg cgcggcgtgt ttaacgtgcc aagttttgtt gccacgctgg gtttatggag
    19261 cgccctgcgc ggaatggggc tgtttatgac caacgccttg ccagtgccga ttgatgaaaa
    19321 cgaagtgctg gactggctgg ggggacaatt tctcggtgtg ccggtatccg cgctgatcat
    19381 gatggtattg tttgcgctgt ttgtgttcat cagccgcaaa accgccttcg ggcgctcggt
    19441 ttttgccgtt ggcggtaatg ccactgcagc acagttgtgc ggtattaacg ttcgtcgggt
    19501 acgcattctt atctttaccc tttcgggatt attagcggcg gtgaccggca ttttgttggc
    19561 ggcgcgcctc ggttccggta acgcaggtgc cgcaaacggt ctggagtttg acgtcatcgc
    19621 cgcggtcgtg gttggcggta cggcgctttc cggtggtcgc ggctcgttgt tcggtacgct
    19681 gcttggcgta ctggtgatta cgctaatcgg taacggtctg gtgctgctcg ggattaactc
    19741 ctttttccag caggtggtgc gcggcgtcat catcgtggtg gcggtgctgg cgaatatctt
    19801 gctgactcag cgtagcagta aagcgaaacg ctaaacctta acgcccctgg cgcgggtgcg
    19861 gcagggatga aaattaatcg tattgttaat gagggattta tgaaaacgat gctggcagct
    19921 tatttaccag gaaattcgac cgtcgatctg cgggaagttg cggtgccgac gccggggatt
    19981 aaccaggtac tgatcaaaat gaaatcctcc gggatttgcg gaagcgatgt ccactatatc
    20041 tatcatcaac accgtgccac ggcagcggca cccgataaac cgttatacca gggatttatc
    20101 aacggtcatg aaccgtgcgg gcagattgtg gcgatggggc aaggctgccg ccattttaaa
    20161 gagggcgacc gtgtgctggt gtatcacatt tctggctgtg gtttttgccc gaactgccgt
    20221 cgcggctttc ctatctcttg tactggcgaa ggaaaagcgg cttacggctg gcagcgtgac
    20281 ggcggtcatg ccgaatatct gctggcggaa gaaaaagatc tgatcctcct gccggatgca
    20341 ctgagttacg aagatggtgc gtttatcagt tgcggcgttg gtacagcgta tgaagggatt
    20401 ttgcgcggcg aagtttccgg cagtgataac gtactggtgg tcggtctggg gccggtcggc
    20461 atgatggcga tgatgctggc gaaaggtcgc ggtgcaaaaa ggatcatcgg cgttgatatg
    20521 ctgccggaac gtctggcgat ggcgaaacag ttaggagtga tggatcacgg ctatttagcg
    20581 accaccgaag gtctgccgca gataatcgcc gaactcactc acggcggcgc ggatgttgcg
    20641 cttgattgtt ccggtaatgc cgcaggtcgc ttactggcac tgcaatccac cgctgactgg
    20701 ggacgcgtgg tttacattgg tgaaaccgga aaagtcgagt tcgaggttag cgccgacctg
    20761 atgcaccatc aacggcggat tatcggctcc tgggtgacca gtctgttcca tatggaaaaa
    20821 tgcgcccacg atttaacgga ctggaaactg tggccgcgta acgccattac ccaccgcttc
    20881 tcgctggaac aggcgggaga tgcttatgca ctgatggcaa gcggcaaatg cgggaaagtt
    20941 gtgattaact tcccggatta aggagttagc ggatgaccat ctatacctta tcccacgggt
    21001 cgctaaagct gggcgtttct gaccagggcg gcgtaatcga aggcttctgg cgcgatacga
    21061 cgccgctctt acgtcctggt aaaaaaaacg gtgtggcaac cgatgcctct tgttttccgc
    21121 tggtgccatt tgcaaaccgg gtgagcggca accgttttgt ctggcagggg cgtgagtatc
    21181 aactgcaacc gaatgttgag tgggatgcgc actatctgca cggcgacggc tggctggggc
    21241 agtggcaatg tgtctcgcgc aatgatgata tcttgtgtct ggtgtatgaa catcgcagcg
    21301 gtgtttatca ctatcgggta agccagacgt ttcatttaac ggcggatacg ctgacggtga
    21361 cactctctgt caccaatcaa ggggcagaga cgctgccatt tggtaccggc tggcatcctt
    21421 attttccgtt gtcgccgcaa acgcggattc aggcacaggc gagcggttac tggctggagc
    21481 gggagcagtg gctggcgggt gagttttgcg agcagctacc gcaagaattg gattttaacc
    21541 agctcgcgcc gttgccgcgc cagtgggtga acaatggttt tgccggatgg aatggtcagg
    21601 cccgcatcga gcagccgcag gagggttatg ccatcatcat agaaacgacg ccacctgcac
    21661 cgtgttattt catctttgtt tccgacccgg cgtttgataa aggatatgcg tttgatttct
    21721 tctgtctgga accgatgagc catgcgccag atgaccatca tcgcccggaa ggtggtgacc
    21781 tcattgcgct tgcaccaggg gaatcaacaa cttcagagat gtcgttgcgg gttgcgttac
    21841 tgtaatttat aaaatgccgg ataaggcgtt cacaccgcat ccggcactcg gcgcacgatg
    21901 cctgatgcga cgcttgccgc gtcttatcag gcctacaatt ggtgttggaa cggtaggcag
    21961 gataaggcgt tcacgccgca tccggcatct ttcctaacgc cgatcgagag aaatcgcccc
    22021 cggcccggta attgccaaca gcaggaatgc gccagcgata ctgacattct tccagaaatt
    22081 aatcatattt ggcccaaccg catcgccggt catatcccag taatggtgac caatcaccgc
    22141 cgtacccagc gtgtagaaaa taaacagcac cgccagcgga cgggtgaaaa agccaagcac
    22201 gattaatatc gcggcgggca cttccataac taccgcaata atcgctgcca gcatcggcat
    22261 cggcgcgccc agcgaggcca tatattggac cgtaccgtca aagcccatca ttttgggaaa
    22321 accaaaaata atgaaaatta agaccacggc gatacgggca attaataaca aaacggggcg
    22381 tgcagcacca aaatcaaaat aacgtagtga gttcataacc aatccctttg caagataatg
    22441 tgatttaaac gtaatacaca ctcttgcacc tcgccacgcc cctggcattg agatattcgc
    22501 taattatcct ggttacagtg atttaagcgc gatgtcctca tctatcagca gtttcgcatc
    22561 aaacgttttg ccgctctgga tccatttgcg gattaagcga atctcacgcc cctgattcag
    22621 cgttaccgca ccgataagca cgccgttttg cagattaaac caaatcgcct tctgagtttc
    22681 cgggttgcca cgacaaatcc agtcatcgcc atgcatatcg ccaataaact gtaagttatc
    22741 actgtactga tcgctccaga accacggcgg cggcagtcgc ggaagcggta gccccaacat
    22801 tgcggcagcg gcaatttgcg cctggttatt ggcgttttcc cagctttcgc agcggtgtag
    22861 tgcaccatta tcaatacggg tgattgccac atcgccaccg gcaaagatcg cgggatcgca
    22921 ggtgcggcaa gcttcatcaa tgacaatacc attggcagta tcaaggttgg cctcgcgagc
    22981 cagttggtcg ttggcgctga taccaatacc gtaaatcacc acatcggccc gaagcgtttc
    23041 gccactttgc agcgtcagtt ctactttttc accatcgacc acatgttcaa tggcattatt
    23101 gagcagaatg cgcacaccag cctgctgatg gcgcggtaaa agatagcgtt gcacgggcgg
    23161 tggtgcatta cggcccatga cgcttgccgc cagttcaatc actgtcacct tacatccacg
    23221 ctgcgtggcg ctggcagcca gttccagacc aatagttccg gcaccgacaa tcacgactga
    23281 ccgttcgggc tgcagaactt ctcgcagtct ggcggcatca ccggcatggc gcagggtaaa
    23341 gcagcgttct cccagtgcat caagcaacgg cagcggtcga gctgccgcgc cggttgctat
    23401 aaaaagctga tcccagtgcc agctttcgcc gttagctaac actaactctt gtgtgtcgcg
    23461 gcccaatgtt ttgatggtta caccggaatg cagatgaaca ttgttttcct gccaccagtg
    23521 agcgggtaac acagactgca actgtgggga atcttccagc aacatggatt tcgagagcgg
    23581 ggggcgttca taaggaagat gttgctcatc ggaaaacaga tgcagctcac cgctgaaccc
    23641 ttgctggcgt agcaaggccg cagccattgc cgccgcttgc ccgccaccga caataatgat
    23701 cgttttttct ttcatcgtgc ttccttaaag atccagccca gccgctacgt ggcgaatgcc
    23761 gcgaatcgct aaacccgcat cagcgttgat catcacaccg cttaatgcgc gattattgcg
    23821 ccgcgatgcc aacatcacat acggacccgt aaaatccgcc ggttgcggga aaaattgcag
    23881 cggtaaaatg gcggcaattt tctccggcgt cagagactgc attatcgagg tttcactttg
    23941 cccgagcgcc tgtgggccgc gcaggtcgct ggccatacca cacgggccga cgccattcac
    24001 ccgcactttc ggtgccagtt cataagccag ttggcgaata agtccggttg cggcatgttt
    24061 actggcggtg tacagcgggc caccgccgcc gggataccag gcggcgtttg acagtgtgaa
    24121 aatcatgctg ccttcactgg cgattaacgc cggagcgcag gcttttgcgc ccagcaggta
    24181 accgagtacg ttgacattaa acagctcgtg gaagccggtt tcgagcgtct ctgcgggagt
    24241 gttaaccagt gaggcattgt gatcccagat gcccgcattg ccgataaaac aatccagctt
    24301 gccagaacga gtcaggatct gattaaccgc gcgttgataa tcggcatagc aggtcacgtt
    24361 accttccacc gccagaatat gttcgccaaa tcgctgacgc agactggcga cttttgccgc
    24421 cgacagttcc agcgtggcaa cctgcgcgcc ttcttcgata aatcgctcgg ccagcgccag
    24481 gcctaatccc gatccgccgc cggtaataaa gatggactcg ttatgcagat cgctcatggc
    24541 tgcgcctccg gtaagtcgat gaagatgtca ccaccttcaa cgtgtactgg ataagtggtt
    24601 agcggatcgg tggcgggcag gcataacgct ttccccgttt tcaggcaaaa actggcggcg
    24661 tgcagcgggc actccaccgt ggcgtcatct tccagatacc cttctgacat cgacgcatta
    24721 ccatggctgc aacgatcgtt aatggcataa aactcgccgc ctacgttgaa cagggcgatg
    24781 acgggcgagg tatcgatccg gagagcctca ccctccggca catccgcaac gggacacgca
    24841 taaattcgat tcatcagaac agtacactca ggttatggga ggagatgacc gcttgatcca
    24901 ggacgatatc ccgttccagc aagcgccagt tcccgtcctc cagacggcga actttgtcga
    24961 aacgcgttcc aacatagaat gtttcatcgc gctctttttg tgcccgataa agtagataat
    25021 tcaccctcac ggcaaatacg tttgggatgt cggtttcgct tacctggcag ttgctgatta
    25081 agtgacgagt gcgtgacggc ggttcttctg cccaggccat gccggtttcc agacgggcga
    25141 ttcgccgttc cagctggtct ttggtgtcat taaaaatcca ggttgtcggc ggttgaacgc
    25201 ctttgcggcg gtcgcgcgtt tgtgcgttaa ccgtggtgcg catggtgtaa cgaatctctt
    25261 cgtcgagctg tgccagccag tcacgaaatt tccagtcatc cagcagactg gcttcgtgaa
    25321 agagaaactg gctaatgcgg tgatgtaact ctagtgaaac ttgcgcactc atttcatcac
    25381 ctcctgctgg taagcggcgg ttttatcgag cacttcctgc cagctttcgc tactcagaag
    25441 atcggcccag cgttggtaca ttccacgagc ggccgtttct gagaagatat agttagtaat
    25501 gcctggaatg ccgtcgtcgc gacgcttttc ctgaccaagc cccatttcca gacacagttt
    25561 gctgttgcgg gcgcggtggc ctttcagcaa tttctggatt tcacaccagt tctccgagtc
    25621 atcctgctcg agaaaaccag caggaccaaa agcacgagtg gcgctgtttt caaaagcggc
    25681 tttaacttca tcggaggcgg ctttgtcagt aatacagaac gcccacactt cgacttgatc
    25741 agggccgcgc ggatgccaga cgcggagcgt ggcagtgccg ttgagccatg aaagcgtggg
    25801 gaaaatattg ttatgacccg ccaggcgcag ggcgcgaact tcacctaaac gttgttctgc
    25861 ttcggcatag gtttcgcggt aatagctgga aaccgcgcca tcgacccaga cattagcatc
    25921 cggtttttca gtaaagaaga aaccgctacc gtgaccgtcc tgaccaaatt gcagcgcatc
    25981 tttggcggtt tcccacaccg ggcgggcggt ttgtccatca ccgagacgct tatcgctgcc
    26041 atcatctttc gcccctaata cctgaacggc agaagcatgg ctgaacagag catgatattg
    26101 gtcactggcg aactgctctg ccgggaattt ccagttgcag ttgatcaccc acttttgtac
    26161 accgccgaca atttcggtgc cgccttcgcg acgatccagc atgccatcca gataccaggc
    26221 aatgtcaccc aggtaatcac gcaggcccgg tgcgctggta tcccagttgc caaaaatcag
    26281 ccctttataa ctctccacac aaggaacttc gtttagtccc cagtgggatt tacacaaccc
    26341 ttgtgggtag gcgcgaggtt ccagcggtac atcgatcaac tcgccgttaa tgccataaga
    26401 ccagccgtga tacggacagg taaaggcgcg agtgttaccg caatctgcat aactcacacg
    26461 catggcccgg tggcggcatt ggttgagaaa cgccttaatg ctgccgtctt tctgacgcac
    26521 tacgacaacc gcatcttccc ccatgtaggt gttaaagaaa tcaccatgtt ttgggatctg
    26581 gctttcgtgg gcgagaaata gccagcaacg accgaaaatg cgctcaagct ccagttggta
    26641 aatatccggg tcggtataaa tacgcggagt gacccgaccg ttttgggtgt caatcagttg
    26701 gtaaatgttc aaatctgagg gtgtggtcat attttttaat cctgaaagat aagatgtatt
    26761 gaccttctat ttttgtgcca taaggatgtt gctggttggt tgctgatatg tgaaatagtt
    26821 aatttctacc agctgagacg ttttttcgat aacaagggga aggtgatgga actacggcat
    26881 ttacgctatt tcgtcgcagt ggcgcaggca ctgaacttta cccgtgcggc ggaaaaactg
    26941 catacctcac agccttcgtt aagcagccag atccgcgatc ttgaaaactg tgtcggtgtt
    27001 ccgttgctgg tgagggataa gcgcaaagtc gcgttgacgg cggcgggaga atgttttctc
    27061 caggacgcgc tggcaatcct cgaacaagcg gaaaatgcca aattacgggc gcggaaaatt
    27121 gttcaggaag acagacaatt aaccattggc tttgtgccat cggcggaagt gaatttactg
    27181 ccaaaagtat taccgatgtt tcgtctcaga cagccagaca ccttaattga gctggtgagt
    27241 ttaatcacca cggaacagga agaaaaaatt cgccgtggtg aactcgacgt cggcttaatg
    27301 cgccatcctg tttatagccc ggagattgat tatctggagc tttttgacga accattagtg
    27361 gtggtgttac cggttgatca ccctttagca catgaaaaag agatcactgc cgcgcaactg
    27421 gatggcgtga atttcgtcag tactgatcca gcgtattccg gttcgcttgc gccgatcgtt
    27481 aaagcgtggt ttgcgcaaga aaacagccag ccaaatatcg tccaggtggc aacgaatatt
    27541 ctggtgacca tgaatctggt ggggatgggg ctgggcgtca ctttgatacc cggttatatg
    27601 aataatttta ataccggcca ggtggtattt cgacccattg ccggaaatgt gcctgccatt
    27661 gctttactga tggcgtggaa gaaaggggag atgaagccgg cattacgcga tttcatcgcc
    27721 attgtgcagg aacgtttggc aagcgtaacg gcataattac gcattatttt tgcaaaacgc
    27781 gtcgcggatc gcagtttttt agtgcttgtg aactggcgtt acagacatcg ggaagcaggc
    27841 tcgaatattc ttatttttca acttgcacca acattcgatt aaccccagac taacgcctcc
    27901 tgacgggagg gactcatggt tttgcaatcc acgcgctggt tggcgctcgg ctatttcaca
    27961 tactttttta gttacggcat ttttctacct ttctggagcg tttggcttaa ggggattggt
    28021 ttaacgccag aaaccatcgg cctgttattg ggggcaggtc tggttgcccg ttttctcggg
    28081 agcttgctca tcgcgccccg cgtcagcgat ccttctcgcc tgatttccgc cttgcgcgtg
    28141 ctggcactgc tgacacttct ctttgctgtc gccttctggg cgggggcgca cgtagcgtgg
    28201 ctgatgctgg tgatgattgg ctttaacctc tttttctcac cgctggtacc gttgaccgat
    28261 gcactggcga atacgtggca aaagcagttc ccgcttgatt acggcaaagt gcgattgtgg
    28321 ggctcggtgg cgtttgtcat tggctcgtcg ctgacgggca aactggtcac tatgtttgat
    28381 tatcgggtga tcctcgcgct gttgacgttg ggcgtggcat ccatgctgct cggctttctc
    28441 atccgtccga cgattcagcc acaaggggca agccgccagc aggagagcac cggttggtct
    28501 gcgtggttgg cgttggttcg ccagaactgg cgctttctgg cctgcgtttg tttattgcag
    28561 ggggcgcatg cggcctatta cggttttagc gccatttact ggcaggctgc tggctactcg
    28621 gcctcggcgg tgggctattt gtggtcgctg ggcgtggtgg cggaagtcat tatctttgcg
    28681 ctgagtaata aacttttccg ccgttgtagt gcacgcgata tgctgttgat ctcagcgatt
    28741 tgcggcgtag tgcgctgggg cattatggga gcaactacgg cgttgccgtg gttgatagtg
    28801 gtgcaaattc tgcattgtgg caccttcacg gtctgccacc tggccgccat gcgctatatt
    28861 gccgctcgcc agggtagcga agtcatccgt ttacaggcgg tttactctgc cgtcgcgatg
    28921 ggcggcagta ttgcgatcat gaccgttttc gccggtttcc tgtatcaata tctgggccac
    28981 ggcgtgttct gggtgatggc gctggtggcg cttccggcaa tgtttttgcg cccgaaagtt
    29041 gttccctcat gctgattcga gcattttgcg gatctgctcc tgttgatgtg ttgtcaacgt
    29101 caggtccgta tagatcagcg gtggtgaaaa gagcggtaac ggcatggtat aaggagcaat
    29161 aatcagtgcc gcgctgcgcg gagcgcctgt ctgcaaaaat gcctttaccg acatatgttt
    29221 aatattgagc ggcagtaacg ttagttcgcg tagctgctgc tcaatctgcg cttctcgctc
    29281 gctatcattc ccggtcagta gaataatctg tttctcatgc aggtcgtttt cctgcattag
    29341 ccaggcacca aaaatcaccg cgaccagacc gctttcctca tcagaaagat ggacgccgta
    29401 ttcactttca aatccggcca gcgccgcgcg ggtggtgcgc accaggcgtg gatacaggcg
    29461 ggcgaactct tccggcaggg tgttatcaat accgatggca aacaaactgc gatttaacgc
    29521 ctgggcgagg tgggtataaa gctgatcgca taacccctgt tcatcataaa aacgaacatt
    29581 tcccagctca cgaaaatggt ttaccagacg tttgatagac ttacgcagtt gtctgtcccg
    29641 ctgatgcgca tcccgcagtg gatcgggaac ccgcaacatg gaaaaaagta atgccataaa
    29701 cagtggctca tcaggcggta caggctggag cgcccgacgc tgccagtggc gtccaatttc
    29761 ctgcgctacc tgaaattcaa ggcaggattc cgcccagcga cgttggagcg gattaaactg
    29821 aggcgtaatt ccagcgtggt gttgcagcaa acaatattgc agatacagac acaggaaatg
    29881 aatatcgcgc gtttcaaagc gtttttgcag ccgtcgggag cagagattca ctaacgcttg
    29941 tagattggta tcgtcgtaaa agttacgcgc aattccgcgc cgcttcagtt cactcttcag
    30001 ggcgggggtg aaatggctgg taataaatga cggacacaga cggaaaccac gtcgtagcca
    30061 gtgaaataaa cacaaacgtt ggttaagcac tgtaccttca acccggtagc taccgtcata
    30121 accagtagtc agtgtgagtt gatgatagcg caggatctcc cgtcctgttt cgctgatatc
    30181 aagactggca atatcatcat ccacaccatt aagctcgctg aaagttgccg tggtggcggt
    30241 caaccccggc tgaaagagcg tcagcaagat ctggcagcgg cgctggggag ccgaaaggac
    30301 agatggtgga gcaagcgtag gcatcatctg tagatctccc ataagcataa ttgataagaa
    30361 tagctaaagt ttgctcaggg aaatggcgaa gggaatgcta atcatcagaa atgttggcaa
    30421 gcgtcacaga atttttctca tcttaaactg ttctgaccag tggattagca tgtcttaagg
    30481 tggttttgtg aatgattatc gtgctagcga tcatgggtta gttctgcgga ttcaacgcgc
    30541 taaggataaa atccaccatc tgttcgcgat aaacatcgcc gtgacgatcg gaaaaagcat
    30601 caatgtgttc gccatccggg attaggatca accgttttgg ctcttttgcc aggctataca
    30661 acttttcgct gtgctgccat gggataacgt gatcagcttt accgtgaatc agtaaaagcg
    30721 ggatcgggct gacgctggcg atgtaatttt cgccgctgta actctcatca agtaagtagc
    30781 cactgccggg gatcatctgg ttggcgatgg tcgcataaga ggcaaatgtg gagtcgagga
    30841 tcaccgcacg tatgccttca cgatcaccct gaccaataac atccaaaata ttcgccccgc
    30901 caatactctg cccgaacagc accagccgtt gtgggtttac atcactgcga tggcgcacca
    30961 cattgatggc actttgcgta tcgtccagca atccggcctg ggacggcgtg ccttttgatt
    31021 taccaaaccc gcgataatca aacataaaaa cgttgaaatt acgctcgggt aaccaactga
    31081 ccagcggcca gtgggcggac atatttccgg cattgccgtg agcatgaata atggtggcaa
    31141 tggccttttc agcggggcca gttgcagaag ggataaacca accatgtagg cgagtgccat
    31201 ctttagcggt gaattctact gattcggctg accagggatc ggggccgtag attttgtcgt
    31261 cagggtaata gaaaaaattg atggcgatgc gcggtattaa ataaatgcag aaggcaacaa
    31321 caaagagaat aaacagaatt ttgagaacgc gtttgttcac tggcagcgcc ataacctttc
    31381 cttgcgactt ctttatcatc cattaatcgg ggaaagatgg tgggagagtg gcgaaagaaa
    31441 gtaaagcggt gaggaagtat ttcggaatag cgcctgaaag gcgagggcgg gtgagtgaca
    31501 tcacccgcca gagtcattaa cgcttcaggg cgtcgcttaa ctcgtcacgc atgttcgcca
    31561 gcatggcttt aacaacgcgc gggttaccag caacgatgtt accggtcagc atgtagttat
    31621 gaccaccggt gaagtcgctg acgatgccgc ccgcttcacg aaccagcagc tcgcctgccg
    31681 cgaaatccca cggacgcaga ccgatttcaa agaaaccgtc aacgcgaccc gcagcaacgt
    31741 aagccagatc cagcgccgca gaaccggtac gacggaagtc tgcacattcg ttgaacagtt
    31801 tgccgacgat gttgatgtag gtagtggcgt actgttttgc tttgaacggg aagccggtcg
    31861 ccagaatagt accgtcgaga tcgcgagcgg tgctgccgcg cagacggtag ccgttcagct
    31921 gtgcgccctg accgcgagtg gcggtgaaca gttcgttacg cataggatcg taaaccacag
    31981 caacttcagt gcggcctttg atacgaacgg cgatagatac cgcgaagtgc ggcagacgtt
    32041 tgataaagtt ggtagtgcca tccagtggat cgataaccca ttgaacatcc tgatcagtac
    32101 cttcaagttc accgctttct tcggtgatga tggtgtgctg tgggtaagat ttacgaatcg
    32161 tgtcgataat caccgcttcg gcagctttat ctacgttggt cacgaagtcg ttactgcctt
    32221 tctggctcgc ttctacagcg tccggggttt catagttttt ggcaattaaa ttacccgcct
    32281 tgcgcgctgc gcgcacggcg atgttcagca tcggatgcat cggtctctct cactggatgt
    32341 taaagaacgg gaaaacggcg cagagtatag cagcgagatc ggcatctgtc ttcgttttat
    32401 gataagatgc gtgaataatc tttagatgaa gaaagacaat gctgcaaaat attcgaattg
    32461 tgctggtgga gacgtcacac accggcaata tggggtctgt tgcccgtgcc atgaaaacaa
    32521 tgggattaac caatctgtgg ctggttaatc cactggtgaa acctgactcc caggcgattg
    32581 ccctggcagc aggggccagc gatgtgattg gtaatgctca catcgtcgat acgctcgacg
    32641 aagcgttagc cggttgtagc ctggtggtgg gcaccagcgc acgctcccgc acgctgccgt
    32701 ggccgatgct cgacccgcgc gaatgcggtc tgaaaagcgt cgctgaagcg gcaaataccc
    32761 cggtggcact ggtgtttggt cgcgagcgcg tcggcttgac caatgaagag ctgcagaaat
    32821 gccattatca tgtcgcgatt gcggctaacc cggaatacag ttcgctgaac ctggcgatgg
    32881 cggttcaggt tattgcctat gaagtgcgca tggcctggct ggcaactcag gaaaatggcg
    32941 agcaggtcga gcatgaagag acgccgtatc cgctggtcga tgatctggag cgtttttatg
    33001 gtcatctgga acaaacgctg ctggcaaccg gttttattcg tgaaaaccat ccggggcagg
    33061 tgatgaataa attgcgccgt ctgtttaccc gtgcgcgccc ggaaagccag gaattgaata
    33121 tcctgcgcgg gattctggct tctatagagc agcagaataa aggtaacaag gccgaataat
    33181 agtggttgcc tgatgcgacg cttaacgcgt cttatcaggc ctgcagtgca cagagcccct
    33241 gggcggataa ggcgttcacg tcgcatccga caacaggtac aaacgccacg ataaaaaaat
    33301 ggcactgaag gttaaatacc cgactaaatc agtcaagtaa atagttgacc aatttactcg
    33361 ggaatgtcag acttgaccct gctatgcaat acccccactt ttacaataaa aaaccccggg
    33421 caggggcgag tttgaggtga agtaagacat gagactgaca tctaaagggc gctatgccgt
    33481 gaccgcaatg cttgacgttg cgctcaactc tgaagcgggc ccggtaccgt tggctgatat
    33541 ttccgaacgt cagggaattt ccctttctta tctggaacaa ctgttttccc gtctgcgtaa
    33601 aaatggtctg gtttccagcg tacgtggacc aggcggtggt tatctgttag gcaaagatgc
    33661 cagcagcatc gccgttggcg aagtgattag cgccgttgac gaatctgtag atgccacccg
    33721 ttgtcagggt aaaggcggct gccagggcgg cgataaatgc ctgacccacg cgctgtggcg
    33781 tgatttgagc gaccgtctta ccggttttct caataacatt actttaggcg aactggttaa
    33841 taaccaggaa gtgctggatg tgtctggtcg tcagcatact cacgacgcgc cacgcacccg
    33901 cacacaagac gcgatcgacg ttaagttacg cgcttaataa aaagaattca gaatcaggcc
    33961 ggagtgctaa acactccgta aacacggtcg tacatccagc cggtagcctg gttccttgca
    34021 ttgagtgatg tacggagttt atagagcaat gaaattaccg atttatctcg actactccgc
    34081 aaccacgccg gtggacccgc gtgttgccga gaaaatgatg cagtttatga cgatggacgg
    34141 aacctttggt aacccggcct cccgttctca ccgtttcggc tggcaggctg aagaagcggt
    34201 tgatatcgcc cgtaatcaga ttgccgatct ggtcggcgct gacccgcgtg aaatcgtctt
    34261 tacctctggt gcaaccgaat ctgacaacct ggcgatcaaa ggtgcagcca acttttatca
    34321 gaaaaaaggc aagcacatca tcaccagtaa aaccgaacat aaagcggtac tggatacctg
    34381 ccgtcagctg gagcgcgaag gttttgaagt cacttacctg gcaccgcagc gtaatggcat
    34441 tatcgacctg aaagaactcg aagcagcgat gcgtgacgac accatccttg tttccatcat
    34501 gcacgtaaat aacgaaatcg gcgtggtgca ggatatcgcg gctatcggcg aaatgtgccg
    34561 tgctcgtggc attatctatc acgttgatgc aacccagagc gtgggcaaac tgcctatcga
    34621 tctgagccag ttgaaagttg acctgatgtc tttctccggt cacaaaatct atggcccgaa
    34681 aggtattggt gcgctgtatg tgcgtcgtaa accgcgcgta cgtatcgaag cacaaatgca
    34741 tggcggcggt cacgagcgcg gtatgcgttc cggcactctg cctgttcacc agatcgtcgg
    34801 tatgggcgaa gcctatcgca tcgcaaaaga agagatggcg accgagatgg aacgtctgcg
    34861 cggcctgcgt aaccgtctgt ggaacggcat caaagatatc gaagaagttt acctgaacgg
    34921 tgacctggaa cacggtgcgc cgaacattct caacgtcagc ttcaactacg ttgaaggtga
    34981 gtcgctgatt atggcgctga aagacctcgc ggtttcttca ggttccgcct gtacgtcagc
    35041 aagcctcgaa ccgtcctacg tgctgcgcgc gctggggctg aacgacgagc tggcacatag
    35101 ctctatccgt ttctctttag gtcgttttac tactgaagaa gagatcgact acaccatcga
    35161 gttagttcgt aaatccatcg gtcgtctgcg tgacctttct ccgctgtggg aaatgtacaa
    35221 gcagggcgtg gatctgaaca gcatcgaatg ggctcatcat taatcggtat cggaatcagg
    35281 agaatttata atggcttaca gcgaaaaagt tatcgaccat tacgagaatc cgcgtaacgt
    35341 gggttccttt gacaacaacg acgagaacgt cggcagcggc atggtgggtg caccggcctg
    35401 tggcgacgtg atgaagttgc agattaaagt caacgatgaa ggtatcattg aagacgcgcg
    35461 ttttaaaact tacggctgcg gttctgctat cgcttccagc tccctggtca ccgaatgggt
    35521 gaaagggaag tctctcgacg aagcgcaggc gatcaaaaac accgatattg ctgaagaact
    35581 tgaactgccg ccggtgaaaa ttcactgttc tattctggca gaagacgcga tcaaagccgc
    35641 cattgcggac tataaaagca aacgtgaagc aaaataagag ttgaggtttg gttatgtcga
    35701 ttacactgag cgacagtgca gcagcgcgag taaatacctt tctggctaac cgcggcaaag
    35761 ggtttggcct gcgtctgggc gtgagaacct ccggatgttc aggtatggct tatgtactgg
    35821 aatttgttga cgaaccgacg ccggaagaca tcgtgtttga agacaaaggc gtgaaagtcg
    35881 tggtcgatgg caaaagcctg caatttctgg acggtacgca gctggacttc gtaaaagaag
    35941 gcctgaacga agggtttaaa ttcaccaacc cgaacgtcaa agatgagtgt ggttgcggcg
    36001 aaagcttcca cgtttgatgc gcataccgat aaccccaccg tggtcgcctg cgcgtggggt
    36061 ttgttttacc tgattcgccg ttatcgcggc ggatcgcagc cctgagaatg ttatggatta
    36121 cttcaccctc tttggcttgc ctgcctgtta tcaactcgat acccaggcgc tgagcctgcg
    36181 ttttcaggat ctacaacgtc agtatcatcc tgataaattc gccagcggaa gccaggcgga
    36241 acaactcgcc gccgtacagc aatctgcaac cattaaccag gcctggcaaa cgctgcgtca
    36301 tccgttaatg cgcgcggaat atttgctttc tttgcacggt tttgatcttg ccagcgagca
    36361 gcatactgtg cgcgacaccg cgttcctgat ggaacagctg gaactgcgcg aagaactgga
    36421 cgagatcgaa caggcgaaag atgaagcgcg gctggaaagc tttatcaaac gtgtgaaaaa
    36481 gatgtttgat acccgccatc agttgatggt tgaacagtta gacaacgaga cgtgggacgc
    36541 ggcggcggat accgtgcgta agctgcgttt tctcgataaa ctgcgaagca gtgccgaaca
    36601 actcgaagaa aaactgctcg atttttaatt tctggaagct aaacatggcc ttattacaaa
    36661 ttagtgaacc tggtttgagt gctgcgccgc atcagcgtcg tctggcggcc ggtatcgacc
    36721 ttggcacaac caactcgctg gtggcgacag tgcgcagcgg tcaggccgaa acgttagccg
    36781 accatgaagg ccgtcacctg ctgccgtctg ttgttcacta tcaacagcaa gggcattcgg
    36841 tgggttatga tgcgcgtact aacgcagcgc tcgataccgc caacaccatt agttctgtta
    36901 aacgcctgat ggggcgctcg ctggctgata tccagcaacg ctatccacat ctgccttatc
    36961 aattccaggc cagcgaaaac ggcctgccga tgattgaaac ggcggcgggg ctgctgaacc
    37021 cggtgcgcgt ttctgcggac atcctcaaag cactggcggc gcgggcaact gaagccctgg
    37081 caggcgagct ggatggtgta gttatcaccg ttccggcgta ctttgacgat gcccagcgtc
    37141 agggcaccaa agacgcggcg cgtctggcgg ggttgcatgt tctgcgctta cttaacgaac
    37201 cgaccgctgc ggctatcgcc tacgggctgg attccggtca ggaaggcgtg atcgccgttt
    37261 atgacctcgg cggcgggacg tttgatattt ccattctgcg cttaagtcgc ggcgtgtttg
    37321 aagtgctggc gaccggcggt gattccgcgc tcggcggcga tgatttcgac catctgctgg
    37381 cggattacat tcgcgagcag gcgggcattc ctgatcgtag cgataaccgc gttcagcgtg
    37441 aactgctgga tgccgccatt gcagccaaaa tcgcgctgag cgatgcggac tccgtgactg
    37501 tgaatgttgc gggctggcag ggcgaaatca gccgtgaaca attcaacgaa ctgatcgcgc
    37561 cactggtaaa acgaaccttg ctggcttgtc gtcgcgcgct gaaagacgct ggcgtagaag
    37621 ctgatgaagt gctggaagtg gtgatggttg gcggttctac tcgcgtgccg ctagtgcgtg
    37681 aacgagtagg cgaatttttc ggtcgtccac cgctgacttc tatcgacccg gataaagtcg
    37741 tcgctattgg cgcggcgatt caggcggata ttctggtggg taacaagccg gacagcgaaa
    37801 tgctgttgct ggatgtgatc ccgctgtcgc tgggcctcga aacgatgggc ggcctggtgg
    37861 agaaagtgat tccgcgtaat accactattc cggtggcgcg cgctcaggat ttcaccacct
    37921 ttaaagatgg tcagacggcg atgtctatcc atgtaatgca gggtgagcgc gaactggtgc
    37981 aggactgccg ctcgctggcg cgttttgcgc tgcgtggtat tccggcgtta ccggctggcg
    38041 gcgcgcatat tcgcgtgacg ttccaggtcg atgccgacgg tcttttgagc gtgacggcga
    38101 tggagaaatc caccggcgtt gaggcatcta ttcaggtcaa accgtcctac ggtctgaccg
    38161 acagcgaaat cgcctcgatg atcaaagact caatgagcta tgccgagcag gacgtaaaag
    38221 cccgtatgct ggcagaacaa aaagtagaag cggcgcgtgt gctggaaagt ttgcacggcg
    38281 cgctggctgc tgatgccgcg ctgttaagca ccgcagagcg tcaggtcatt gacgatgctg
    38341 ccgctcacct gagtgaagtg gcgcagggcg atgatgttga cgccatcgaa caagcgatta
    38401 aaaacgtaga caaacaaacc caggatttcg ccgctcgccg catggaccag tcggttcgtc
    38461 gtgcgctgaa aggccattcc gtggacgagg tttaatatgc caaagattgt tattttgcct
    38521 catcaggatc tctgccctga tggcgctgtt ctggaagcga atagtggtga aaccattctc
    38581 gacgttgcgc tgcgtaacgg tatcgagatt gaacacgcct gtgaaaaatc ctgtgcttgc
    38641 accacctgcc actgcatcgt tcgcgaaggt tttgactcgc tgccggaaag ctcagagcag
    38701 gaagacgaca tgctggacaa agcctgggga ctggagccgg aaagccgttt aagctgccag
    38761 gcgcgcgtta ccgacgaaga tttagtggtc gaaatcccgc gttacactat caaccatgcg
    38821 cgtgagcatt aacagaggtt agtatgggac ttaagtggac cgatagccgc gaaattggcg
    38881 aagcactgta cgatgcgtat cccgatcttg atccgaaaac ggttcgattc accgatatgc
    38941 atcagtggat ttgcgatctg gaagatttcg acgacgaccc gcaggcatcg aacgagaaaa
    39001 tcctcgaagc gattttgttg gtctggctgg acgaagccga gtgaatgaac gggctgcctg
    39061 ggggtagccc gttttgctgt taaacgaata aggataacta aaatgacaga agcgatgaaa
    39121 attacccttt ctacccaacc tgccgacgcg cgctggggag aaaaagcgac ttacagcatt
    39181 aataatgatg gcattaccct gcatttgaac gggacagacg atctggggct gatccagcgt
    39241 gcggcgcgca agattgacgg tctgggcatc aagcatgttc agttaagcgg tgaagactgg
    39301 gatgcggatc gctgctgggc attctggcaa ggttacaaag ccccgaaagg cacacgtaaa
    39361 gtggagtggc cggatctgga cgatgcccaa cggcaggaac tggataaccg cctgatgatc
    39421 atcgactggg tgcgtgacac catcaacgca ccggcggaag aactggggcc atcgcaactg
    39481 gcacagcgtg ctgttgatct gatcagcaac gtcgcgggcg atcgtgtgac ttaccggatc
    39541 accaaaggcg aagatctgcg tgatcaaggt tatatggggc tgcacacggt cgggcgcggt
    39601 tcagaacgtt ccccggtatt gctggcgctg gattacaacc caacaggcga taaagaagcg
    39661 ccagtgtacg cgtgcctggt tggtaaaggc atcacctttg attccggcgg ctacagtatc
    39721 aaacagaccg catttatgga ctcgatgaag tcggacatgg gcggcgcggc aacggttacc
    39781 ggagcgctgg cgtttgccat tacgcgcgga ctgaacaagc gcgtgaagct gttcctctgc
    39841 tgtgcggata acctgatcag cggcaatgcg ttcaagctgg gcgatatcat tacctatcgc
    39901 aacggtaaaa aagttgaagt gatgaacacc gatgcggaag ggcgtctggt gctggccgat
    39961 ggcctgattg atgccagtgc gcagaaaccg gaactgatca ttgatgcggc gaccctcacc
    40021 ggggcggcga aaaccgcgct gggtaatgat tatcacgcgc tgttcagttt tgacgatgcg
    40081 cttgccgctc gcttgctggc gagtgccgcg caggagaatg aaccattctg gcgtctgccg
    40141 ctggcggagt tccaccgcag ccagctgccg tctaactttg ccgaactgaa caataccgga
    40201 agcgcggcgt atccggcagg tgcaagcacg gcggcgggct tcctgtcgca ctttgttgag
    40261 aactatcagc aaggctggct gcatatcgac tgctcggcga cttaccgtaa agcgccggtt
    40321 gaacagtggt ctgcgggcgc gaccgggcta ggtgtgcgca cgattgctaa tctgttaacg
    40381 gcgtaaacgt cttatttggc ctacaaaatt gcgaaaatcc aatagattga gattcccttg
    40441 taggcctgat aagcgtagcg catcaggcac ttgtgccccg aacgttgaat gaattgtggt
    40501 tcttatgtcc gaaacaaaaa acgaacttga agacctgctg gaaaaagcag caaccgaacc
    40561 ggcgcaccgc ccggcctttt tccgtactct actggaatcc accgtctggg tgccgggtac
    40621 ggcggcgcag ggcgaggctg tggttgaaga tagcgcgctt gatttacagc actgggaaaa
    40681 agaagacggc accagcgtca ttcctttttt tacctcgtta gaagcacttc aacaggcggt
    40741 tgaagacgaa caggcatttg tcgtaatgcc cgttcgcaca ttgtttgaga tgacccttgg
    40801 cgaaacgctc ttccttaatg ccaaactgcc aaccggtaaa gaatttatgc cgcgtgaaat
    40861 cagtttgctg attggtgaag aaggaaatcc gctgagcagc caggaagtac tggaaggcgg
    40921 tgaatcgctg atattatcgg aagtcgcaga gccaccagca caaatgattg attcactcac
    40981 caccttattt aaaactatta agccggtgaa gcgtgctttt atttgttcaa ttaaagagaa
    41041 cgaagaggca cagcctaatt tacttattgg cattgaagcc gatggtgata tcgaagaaat
    41101 tattcaggcg gcgggaagtg tagcgaccga tacattacct ggcgatgaac ccatcgatat
    41161 ttgtcaggtg aaaaaagggg aaaaaggaat tagccacttt attaccgaac atattgcgcc
    41221 attctatgaa cgtcgctggg gtggtttttt gcgtgacttt aaacagaacc ggataatcta
    41281 aaatatgccg ccccaaaggg cggcattcgg gcttacttgc tgctctgaag gttgttaacc
    41341 gccagtaaat tgttgccgga gagactccgg ttattattta cctcgcttaa cagcgcacca
    41401 gaaacaataa gtacgataca aaagcaccgt atcttcattg tgcggtccat gcgaagaccg
    41461 catcacgaca gcaccactgg cgatattgcc gcgatagcaa gcgctgagac gcgcttgctc
    41521 taacggtcgc ggaacatcct ttccttaagc cagcgcccgc ttgcgagacg gcggaaaggt
    41581 ccgcagtgga taatacacta acagaacaat aataactctc ttttacttga caaaaagaga
    41641 gttactggcg agtattgtgt ccgctgagac gcgcttgcat tactacggta atacaatctg
    41701 ctggaaaggc cgcgaaccaa accagcaaca aataaccgcc tgattttggc ggttttttgt
    41761 tttttaaagg aaagtgaatt tacatgttta agtaatatct tttaccggtt agtattttta
    41821 attttgagca ggatttgttt atatttgtct gaattcggac taataagcca ggttatttta
    41881 ttaccgaaca cggcggaaag ggccggtgtc gataatacgc taaataaaca atgattactc
    41941 tctttcgctt gacaaaaaga gagttactgg cgagtattgc tttgctgtga agcacctgcg
    42001 ttgctcatgc aacacaagcc aatctgctgg aaaggccgcg aaccagacca gcaataaaaa
    42061 accgccagat tcggcggttt tttattgcct gttaaaagct tatttctctg gctcaactgg
    42121 taaatctgct cgtgcgcccc attcactcca tgcgccgtcg tacagtttca cgtttggcac
    42181 atcaagtgtc gcgagtgcca acaaaaccac ggctgccgtt acaccagagc cgcagctgac
    42241 gataataggt ttgtcataac ttacgccgcg accaaaaaat atcgcatcca gttcatcggt
    42301 cgtttttagt tcaccttcgc gcaccagttc cgtccacgga acatttagtg caccgggaat
    42361 atgtccgcga cgtaaacctg ggcgaggttc atcaacttct gcgttaaaac gtgcagccgg
    42421 gcgggcatcg ataatttgcg ccgtattttc atggcttgcc aataacacat cggttacttt
    42481 caccacggct tcaggattaa acgcggcgtt aaactctcct tccggcagct ctactgcgcc
    42541 ttcttctaat agcagatcat cacgctgcca gcctgcaagt ccaccaccca gaatcgacac
    42601 tttctctaca ccaaaggtgc gcagcatcca ccatgctcgt ggggctgaga aaagattacc
    42661 ttcgtcatag acaatcaggt gcttatcctg gttaacgcct aattcacgca tcgccacggc
    42721 gaacgtttcc gggcgcggca gcatgtgcgg aagcggggaa gtgtgatcag aaagcgcttc
    42781 tatatcaaaa aacactgcgc cgggaatatg tccattcaga tactcctgag caacgttacg
    42841 atcttcctgt ccaggcgacg ccatgcgggc atcgataatc tgaatttccg ggtcatcaat
    42901 atgttcggcg agccagtcgg ctcctacaaa ccatgtcgtg gacataggca tctccatttt
    42961 tgccggggtt aggctaaatt gtcggatgtt tagtgaaagg cgacaagcaa aagaggctga
    43021 tttggctgtc tgagccacaa ttctcactaa aattcggatt gtatgggcgt gatactgaat
    43081 tacataacgt aaattctcac gcataataat tattcgacgt tgctaacggg ccattgtggc
    43141 agggatgaaa aatgaaaaag ttacgcgtag ccgcctgcat gctaatgctg gcgctggcag
    43201 ggtgcgacaa caacgataac gcgccaacag cggtgaaaaa agatgcgcct tctgaagtta
    43261 ctaaagcggc ctcttcagaa aacgtgagtt cagcaaaact ttccgcgcca gagcgacaaa
    43321 aactggccca acagagtgcc ggtaaggcgc tgacattgct ggatctctct gaagttcaac
    43381 ttgatggcgc agccacgctg gtgctgacat tctccatccc tctcgacccg gatcaggaat
    43441 tctcacgcgt tatccatgtc gtcgataaga aaagcggcaa agtggatggt gcctgggagc
    43501 tgtcagataa tcttaaagag ttgcgtttac gccacctcga accgaaacgt gatttgatcg
    43561 tcactattgg caaggaggtc aaagcactca acaacgcaac cttcagtaaa gattacgaaa
    43621 aaactataac tacccgagat atccaaccca gcgtcggttt tgccagccgt ggctcgttgt
    43681 tacctggtaa agttattgaa gggctgccgg taatggcgct caacgttaac aatgtcgatg
    43741 ttaacttctt ccgtgttaag ccagaatctc tgcctgcatt cattagccaa tgggaatacc
    43801 gcaattcgct ggcgaactgg cagtctgaca aactgctgca gatggcggat ctggtttata
    43861 ccggacggtt tgatctcaat tctgcgcgta acacccgtga aaaattattg ctgccgctgg
    43921 gcgatatcaa accgcttcag caggcaggcg tgtatctggc tgtgatgaat caggctggac
    43981 gttacgatta cagtaatccc gcgacgctgt ttacgttaag tgatatcggc gtttcagctc
    44041 accgttatca caatcgtctg gatatcttta cccaaagcct ggaaaacggc gcggcccagc
    44101 aaggagttga agtctcatta ttaaatgaga aagggcagac tctgactcag gcaaccagtg
    44161 atgcccaggg gcatgtgcag ctggaaaatg ataaaaacgc ggcattactg ttggcgcgta
    44221 aagacggtca gacaacgcta ctcgatttaa aacttccggc gctggactta gcagaattta
    44281 acattgctgg cgcgccaggc tatagcaaac agtttttcat gtttggcccg cgcgatcttt
    44341 atcgccctgg tgaaacagtc atcctcaatg gtttattgcg tgatgcagat ggtaaagcat
    44401 tgcccgatca acccatcaag ttagacgtga ttaaacccga cgggcaggta ctcaggagcg
    44461 tcgttagtca gccggagaat ggcctctacc actttacctg gccactcgat agcaatgcgg
    44521 caaccggtat gtggcatatt cgcgctaaca cgggcgataa ccagtatcgg atgtgggatt
    44581 tccacgtcga agattttatg ccagagcgca tggcgctgaa tctgaccggt gagaaaaccc
    44641 cgctaacgcc gaaagatgaa gtgagattct ccgtggtggg gtactacctg tatggtgcgc
    44701 ctgctaatgg taatactttg caagggcaac ttttcctgcg cccactgcgt gaagctgtgt
    44761 cagccttacc tggtttcgaa ttcggcgata tagctgccga aaatctttcc cgcacgctgg
    44821 atgaagttca gttgacgctg gatgataaag ggcgtggcga agtttctaca gaaagccagt
    44881 ggaaggaaac gcattcccca ttacaggtta ttttccaggg tagtttgttg gaatcgggcg
    44941 gtcgcccggt gacgcgccgc gctgagcagg ctatctggcc tgccgatgca ttgccgggga
    45001 tccgtccgca gttcgcctcg aaatcggttt acgattatcg cactgacagc acggtgaaac
    45061 agcccattgt tgatgaaggc agtaacgccg cttttgacat cgtttatagc gatgcgcaag
    45121 gcgtaaaaaa agccgtgtcg ggcttgcagg tgcgcctgat tcgcgaacgc cgcgattact
    45181 actggaactg gtcagaagat gaaggctggc agtcacagtt tgatcaaaaa gatctgatcg
    45241 aaaatgaaca aactctggat ctgaaagcgg acgaaaccgg caaggtcagt ttcccggtag
    45301 agtggggcgc ttatcgtctg gaagtcaaag cgccgaatga agcggtcagt agtgtgcgtt
    45361 tctgggctgg ctatagctgg caggacaaca gcgacgggag cggtgccgtg cgacccgacc
    45421 gtgtcacgct gaaactggat aaagccagct atcgccctgg cgataccatt aagttgcata
    45481 ttgccgcgcc aacggcgggt aaaggttatg cgatggtcga gtccagtgaa gggccgctgt
    45541 ggtggcaaga gattgatgtt ccggctcaag ggctggatct gacgattccg gtcgataaaa
    45601 cctggaatcg tcatgatctg tatttaagta cgctggtggt acgtcctggc gataaatctc
    45661 gctccgcgac gccaaaacgc gcggttgggg tgttgcatct gccgcttggt gatgaaaacc
    45721 gtcgcctcga tctggcgctg gaaaccccta ccaaaatgcg ccccaatcag ccattaaccg
    45781 tgaaaattaa agccagcaat aaaaatggtg agatgccaaa acaggtgaat gtgctggtgt
    45841 ctgccgttga tagcggtgtg ctgaatatta ctgattacgt cacgccagat ccgtggcagg
    45901 cgttctttgg tcagaaacgc tatggcgcag acatttacga tatttacggt caggtcattg
    45961 aaggtcaggg gcgtctggca gctctgcgtt ttggtggtga tggtgatgag ctgaaacgtg
    46021 gtggtaaacc gccggtcaat cacgtcaata ttgtcgcgca gcaggcgctg ccggtaacgc
    46081 tcaacgaaca gggcgaaggc tcggttacac tgccaattgg cgattttaac ggtgaattgc
    46141 gcgtcatggc gcaagcctgg acggcagatg acttcggaag caacgaaagc aaagtgattg
    46201 ttgctgcacc ggtgattgct gaactgaaca tgccacgctt tatggcgagt ggcgatacct
    46261 cgcgcctgac gctggatatc actaatctta ccgataaacc gcaaaaactt aatgttgccc
    46321 tgaccgccag tggtttgctt gaactggtca gcgattcacc cgcagccgtt gaattagcgc
    46381 caggggtgcg tactacgctg tttatcccgg tgcgtgcatt gccgggttat ggcgatggag
    46441 acattcaggc caccattagc gggttagcgt tacccgggga aaccgttgcc gatcagcata
    46501 agcagtggaa aatcggcgtc cgtccggcat tcccggcaca aacggtcaat tacggtacgg
    46561 cgttacagcc tggtgaaacc tgggcgctcc cggctgatgg attgcaaaac ttctcgcctg
    46621 ttacgctgga agggcaattg ttgttgagcg gcaaaccacc gctgaacatc gcacgttata
    46681 tcaaagagtt aaaagcgtat ccgtacggct gtctcgagca aaccaccagc ggcctgttcc
    46741 cgtcacttta taccaatgca gcccaactgc aggcgttggg catcaaaggc gacagtgatg
    46801 agaaacgccg tgcatcggtc gatatcggca tttcccgttt gctgcaaatg caacgtgata
    46861 acggcggctt tacgctgtgg gataaaaacg gtgacgaaga gtactggctg acggcttacg
    46921 tgatggattt cctggtccgc gcaggcgaac agggttacag cgtaccgaca gacgccatta
    46981 accggggtaa tgagcgtctg ctgcgctatt tacaagatcc gggcatgatg tcgatcccgt
    47041 acgctgataa tctcaaagcc agtaaattcg ccgtacagtc ttacgctgcg ctggtgttgg
    47101 cccgtcagca aaaagcacca ctgggtgcgc tgcgtgaaat ctgggagcat cgtgcagatg
    47161 ccgcttctgg tttaccgctg ctgcaacttg gcgttgcgct gaaaaccatg ggtgatgcaa
    47221 tgcgtggtga agaagcgatt gtgctggcgc tgaaaacgcc gcgtaatagt gatgagcgga
    47281 tatggctggg tgattacggt agtccactgc gcgacagcgc gttgatgctc tcattgctgg
    47341 aagagaacaa actgctaccc gatgagcagt actccctgtt gaacacactt tcgcagcagg
    47401 cgttcggtga acgctggcta tcgacgcagg aaagtaacgc gttgttcctg gctgcccgta
    47461 ctcttcagga tttaccgggc aaatggcagg cgcaaaccac tttctctgct gagccgctga
    47521 caggcgagaa agcgcaaacc agcaatctga atagcgatca acttgccacc ttgcaggtga
    47581 ccaacagtgg cgatcagccg ttatggctgc gtgtggatgc cagcggttat ccgcaatccg
    47641 cacctttatc ggcgagcaat gtgctgcaaa ttgagcgtca tattctgggt actgacggta
    47701 agagcaaatc gctggactcg ttacgtagcg gcgatctggt gctggtgtgg ttgcaggtta
    47761 aagccagtaa cagtgtaccg gatgcgttag tcgtggatct gttgcctgcg gggctggaac
    47821 tggaaaacca gaatctggcg aacggcagcg ccagcctgga gcaaagtggt ggtgaagtgc
    47881 agaacttgct gaaccagatg cagcaggcga gcattaagca catagagttc cgtgacgatc
    47941 gctttgtggc ggcggttgcc gttgatgaat atcaaccggt aacgctggtg tatctggcgc
    48001 gggcggtgac gccgggaacg tatcaggtgc cgcagcctat ggtggaatca atgtatgttc
    48061 ctcaatggcg ggcgactggt gcggctgacg atctgttgat agtcagaccg taaatgcctc
    48121 gcctgttaac caaacgcggc tgctggataa tgctggcagc cgcgcctttt atcatcattc
    48181 tggcagcatg ggcagcagat aaactctggc ctctgccatt gcaagaagtg aatcctgcac
    48241 gcgtggtggt ggcgcaggat ggtacgccac tctggcgttt cgccgatgct gacggtatct
    48301 ggcgttatcc ggtaacaatc gaagatgttt ctccacgtta ccttgaagcg ctgatcaatt
    48361 atgaagatcg ctggttctgg atgcatccgg gggtgaatcc gttctcggtg gcgcgtgcag
    48421 cctggcaaga tctcacgtcg ggtcgggtca tttccggcgg cagcacgctc accatgcagg
    48481 tggcgcgcct gcttgatcct caccccaaaa cgtttggcgg caaaattcgc cagctctggc
    48541 gcgcgttgca actggaatgg catctgtcta aacgtgaaat tctgaccttg tatcttaacc
    48601 gtgccccgtt tggcggtacg ttgcagggga ttggtgcggc aagttgggct tacctcggga
    48661 aatcgcctgc gaatttaagc tattccgagg cggcaatgct ggcggttttg ccgcaagcgc
    48721 ccagccgtat tcgcccggat cgttggccgg agcgtgccga agccgcgcgt aataaagtgc
    48781 tcgaacggat ggccgtgcaa ggtgtgtggt cccgtgagcg ggtaaaagag tcaagggaag
    48841 aacccatctg gctggcaccc cgacaaatgc cgcaactggc actgctgttt tcgcgcatga
    48901 tgctcggtaa aagcaaaagc gacaaaatcg ttactacgct ggatgccggt cttcaacgac
    48961 gtctggaaga actggcgcaa aactggaaag gacgattgcc accgcgcagc tcactggcga
    49021 tgatcgtggt tgatcatacc gatatgcgtg ttcgcggctg ggtgggatca gttgatctca
    49081 acgatgattc acgctttggt catgttgata tggtcaatgc gatccgatcg ccaggatcgg
    49141 tgctcaaacc gtttgtttat ggtctggcgc tggatgaagg cttgatccac ccggcatcgc
    49201 tgttgcaaga cgtccccaga cgcaccggtg attaccgacc tggcaacttt gacagcggtt
    49261 ttcatggccc gatcagcatg agtgaggcgc tggtgcgatc gctgaactta cctgctgtgc
    49321 aggtgctgga agcctatgga ccgaaacggt ttgcggcaaa gttacgcaat gttggattgc
    49381 cgttatattt gcccaacggt gctgcaccga atctttcgct cattctcggc ggcgctggcg
    49441 caaaactgga agatatggcg gcagcgtata ccgcgtttgc tcgccacggc aaggcaggca
    49501 aattgcgctt acagcctgac gatccgctgc ttgaacggcc tttaatgtcg tcgggggctg
    49561 cgtggatcat tcgccggatt atggctgatg aagcgcaacc cttgccggat ggtgccttgc
    49621 cgcgcgtcgc cccactggca tggaaaacgg gcaccagcta tggctatcgt gatgcctggg
    49681 cgattggggt taacgctcgc tatgtcattg ggatctggac tggcagaccg gacggcacgc
    49741 ccgttgttgg tcagtttggc tttgccagtg ccataccgct gttgaatcag gttaataata
    49801 tcttactgtc gcgcagtgtg aatttgccgg aagacccacg tcctgactct gtaagtcgtg
    49861 gcgttatctg ctggccgggc ggacagtctt tggcggaagg tgacggtaac tgtcgccgcc
    49921 gcctggcaac ctggctgctg gacgggagcc agccgccaac tctattactg ccggagcagg
    49981 aaggcattaa tggcattcgt ttccccatct ggctggatga aaacggcaaa cgtgttgccg
    50041 ctgattgccc tcaggcgcga caagaaatga taaatgtctg gccgctaccg ctggaaccct
    50101 ggctgcccgc atcagaacgt cgcgctgtgc gcttgccacc ggcctcgaca atttgcccgc
    50161 catacggtca cgatgcacaa ctcccgctgc agctaacagg tgtgcgcgat ggcgcgatta
    50221 ttaaacgttt accgggcgca gcggaagcga ctttgccgtt gcaatcaagt ggaggggcag
    50281 gtgaacgctg gtggtttctg aacggcgaac cgttaactga acgtgggcgc aacgtgaccc
    50341 tgcatttgac ggataaaggc gattatcaat tgctggtgat ggatgaggtg gggcaaatag
    50401 cgacagtgaa atttgtcatg caatagtctg ttttgtccct gattgttgct taaactgggt
    50461 ttattttaaa aaaatgttac cttcatcaat agtcaacggc tctgttgctc attataatcc
    50521 gcgccatctc gtacgctggt acagacaaca acagaacaat ttacagaggt aaaaatggct
    50581 attgaacgta ctttttccat catcaaaccg aacgcggtag caaaaaacgt gattggtagt
    50641 atctttgcgc gctttgaagc tgcagggttc aaaattgtcg gcaccaaaat gctgcacctg
    50701 accgttgaac aggctcgtgg cttttatgct gaacacgatg gaaaaccgtt ctttgatggt
    50761 ctggttgaat tcatgacttc tggcccgatc gttgtttccg tgctggaagg tgaaaatgct
    50821 gttcagcgtc accgcgatct gctgggcgcg accaatccgg caaacgcact ggccggtact
    50881 ctgcgtgctg attacgctga cagcctgacc gaaaatggta cccacggttc tgattccgtc
    50941 gaatctgccg ctcgcgaaat cgcttatttc ttcggcgaag gcgaagtgtg cccgcgcacc
    51001 cgttaataat ttcgtaaatg ccgcgtgcaa acgtggcatc cgtgcgccag aatttgtaca
    51061 atgcagcgcc cccggacgag cagccgctca ccggggcgtt tcttttttca accctccagg
    51121 ggccataacg tgtaataacg aggccggaat agcatatgtc tgaacaatta gtcacacctg
    51181 aaaacgtcac cactaaagat ggaaaaatca acctgctgga tctcaaccgt cagcagatgc
    51241 gggagttttt taaagattta ggtgaaaaac ccttccgcgc cgatcaggtg atgaagtgga
    51301 tgtatcacta ttgctgcgac aactttgatg agatgaccga catcaacaaa gtgttgcgcg
    51361 gcaaactgaa agaggtggcg gagatccgcg caccggaagt ggttgaagaa cagcgttcat
    51421 ctgacggcac cattaaatgg gcaattgccg ttggcgatca gcgcgtcgaa acggtgtata
    51481 tcccggaaga cgaccgtgcc acgctctgcg tctcttcgca ggtggggtgt gcgctggagt
    51541 gtaaattctg ttccaccgcc cagcagggct ttaaccgcaa cctgcgggta tcggaaatta
    51601 tcggccaggt gtggcgtgcg gcgaaaattg tcggcgcagc aaaagtcacc ggtcagcgcc
    51661 caatcactaa cgtcgtgatg atgggtatgg gcgagccgct gctcaacctg aacaacgtcg
    51721 ttccggcgat ggaaatcatg cttgatgatt tcggttttgg cctgtcaaaa cgtcgcgtca
    51781 cgctttccac ttccggtgtg gttccggcgc tggataaact gggcgatatg atcgacgttg
    51841 cgctggctat ctccctgcac gcgccgaacg acgaaattcg tgacgaaatt gtgccgatca
    51901 acaaaaagta caatatcgaa acgttccttg cggcggtgcg ccgttatctg gagaaatcca
    51961 acgccaatca gggccgagtc actattgaat acgtgatgct tgaccacgtt aacgacggca
    52021 ctgaacacgc gcaccaactg gcggaactgc tgaaagatac gccgtgtaag atcaacctga
    52081 tcccgtggaa cccgttcccg ggcgcgccgt atggacgcag ttcgaacagc cgtatcgacc
    52141 gtttctcaaa agtgctgatg agctacggtt tcaccactat tgtgcgtaaa acgcgtggtg
    52201 atgacattga tgctgcctgt ggtcagttgg cgggcgatgt tatcgaccgt acgaaacgta
    52261 ccctgcgtaa acgaatgcag ggtgaagcca tcgacattaa agcggtctga tagccattac
    52321 gccacggtta ctttatgtac cgtggcgtaa tcttttatga atgaagtatt aagttgcaat
    52381 tgtctggtca atattaacgg tgcgtttttg ctgactttaa ggcagtatgt aacgatgcaa
    52441 cagtaagtta gccttagcga atgtgggctt tgtcacgagc acacagacgg tcttatactg
    52501 tatgataacg gttaacttaa cggatgtttc gcggtgtggg tgggcatgat ggttcaccgg
    52561 catctcaatt ctcatttaaa cgtacctgca gcgaatgaat actgaagcca cgcacgacca
    52621 aaatgaagca cttactaccg gcgctcgcct gcgtaatgct cgcgaacaac taggacttag
    52681 tcaacaggcc gttgccgagc gactttgcct gaaggtttcc acggtacgcg acattgaaga
    52741 agataaggca cccgccgatc ttgcttcaac attcctgcgc ggatatatcc gctcttatgc
    52801 gcgtctggta catattccag aagaagaact gctgccaggg ctggaaaagc aggctccact
    52861 acgggctgca aaagttgcgc cgatgcagag tttttccctc ggtaaacgcc gcaaaaaacg
    52921 tgacggctgg ctgatgacct tcacctggct ggtattgttt gtggttattg gcctgagcgg
    52981 tgcctggtgg tggcaagacc acaaagctca gcaggaagag atcaccacta tggccgatca
    53041 atcttctgcg gaactgaata ataatcaaag ccagagtgta ccgttagata cgtcgacaac
    53101 cacagatcag gctatggcga ccacgccgac atcacctgtg gataccaccg caaccaacac
    53161 gcaaacacct gccgtaactg cgccagcacc agctgttgat ccgcaacaga atgcggttgt
    53221 tccaccttcg caggcaaatg ttgataccgc tgcgactccg gcaccagcgg caacaacaac
    53281 accagatggc gctgcgccgt tgccaaccga tcaggctggc gtgaccacgc cggcggttga
    53341 cccgaatgcg ctggtgatga actttactgc cgattgctgg ctggaagtca ccgatgctac
    53401 cggtaaaaaa ctgtttagcg gtatgcagcg taaagacggt aatttgaact taaccggcca
    53461 ggcaccgtac aaactgaaaa ttggtgcgcc agccgcagta cagatccagt atcaagggaa
    53521 acctgtcgat ctgagtcgtt ttatcagaac taaccaggtt gcgcgtctga ccctcaatgc
    53581 cgaacaatca ccggcgcagt aacagacggg taacgcggga gatttttcat gcataaccag
    53641 gctccaattc aacgtagaaa atcaacacgt atttacgttg ggaatgtgcc gattggcgat
    53701 ggtgctccca tcgccgtaca gtccatgacc aatacgcgca cgacagacgt cgaagcaacg
    53761 gtcaatcaaa tcaaggcgct ggaacgtgtt ggcgctgata tcgtccgtgt ctccgtaccg
    53821 acgatggacg cggcagaagc gttcaaactc atcaaacagc aggttaacgt gccgctggtg
    53881 gctgacatcc acttcgacta tcgcattgcg ctgaaagtag cggaatacgg cgtcgattgt
    53941 ctgcgtatta accctggcaa tatcggtaat gaagagcgta ttcgcatggt ggttgactgt
    54001 gcgcgcgata aaaacattcc gatccgtatt ggcgttaatg ccggatcgtt ggaaaaagat
    54061 ctgcaagaaa agtatggcga accgacgccg caggcgttgc tggaatctgc catgcgtcat
    54121 gttgatcatc tcgatcgcct gaacttcgat cagttcaaag tcagcgtgaa agcgtctgac
    54181 gtcttcctcg ctgttgagtc ttatcgtttg ctggcaaaac aaatcgatca gccgctgcat
    54241 ctggggatca ccgaagccgg tggtgcgcgt agcggggcag taaaatccgc tattggttta
    54301 ggtctgctgc tgtctgaagg catcggcgac acgctgcgcg tatcgctggc ggccgatccg
    54361 gtcgaagaga tcaaagtcgg tttcgatatt ttgaaatcgc tgcgtatccg ttcgcgcggg
    54421 atcaacttca tcgcctgccc gacatgttcg cgtcaggaat ttgatgttat cggtacggtt
    54481 aacgcgctgg agcaacgcct ggaagatatc atcactccga tggacgtttc gattatcggc
    54541 tgcgtggtga atggcccagg tgaggcgctg gtttctacac tcggcgtcac tggcggcaac
    54601 aagaaaagcg gcctctatga agatggcgtg cgcaaagacc gtctggacaa caacgatatg
    54661 atcgatcagc tggaagcgcg cattcgtgcg aaagccagtc agctggacga agcgcgtcga
    54721 attgacgttc agcaggttga aaaataataa cgtgatggga agcgcctcgc ttcccgtgta
    54781 tgattgaacc cgcatggctc ccgaaacatt gagggaagcg ttgagggttc atttttatat
    54841 tcagaaagag aataaacgtg gcaaaaaaca ttcaagccat tcgcggcatg aacgattacc
    54901 tgcctggcga aacggccatc tggcagcgca ttgaaggcac actgaaaaac gtgctcggca
    54961 gctacggtta cagtgaaatc cgcttgccga ttgtagagca gaccccgcta ttcaaacgtg
    55021 cgattggtga agtcaccgac gtggttgaaa aagagatgta cacctttgag gatcgcaatg
    55081 gcgacagcct gactctgcgc cctgaaggga cggcgggctg tgtacgcgcc ggcatcgagc
    55141 atggtcttct gtacaatcag gaacagcgtc tgtggtatat cgggcctatg ttccgtcacg
    55201 agcgtccgca gaaagggcgt tatcgtcagt tccatcagtt gggctgcgaa gttttcggtc
    55261 tgcaaggtcc ggatatcgac gccgaactga ttatgctcac tgcccgctgg tggcgcacgc
    55321 tgggtatttc cgagcacgta actcttgagc tgaactctat cggttcgctg gaagctcgtg
    55381 cgaattaccg cgatgcgctg gtggcattcc ttgagcagca taaagaaaag ctggacgaag
    55441 actgcaaacg ccgcatgtac actaacccgt tgcgcgtact cgattcaaaa aatccggaag
    55501 tgcaggtgct tctcaacgac gctccggcat taggcgatta tctggacgag gaatctcgtg
    55561 agcactttgc cggtctgtgc aaactgcttg agagcgcggg tatcgcttac accgtaaacc
    55621 agcgtctggt gcgcggtctg gattactata accgtaccgt tttcgagtgg gtgactaaca
    55681 gcctcggttc ccagggcacc gtgtgtgcag gtggtcgtta tgacggtctg gtggaacaac
    55741 tgggtggtcg tgcaacaccg gcagtcggtt ttgcgatggg cctcgaacgt cttgtattgt
    55801 tagttcaggc cgttaatccg gaatttaaag ccgatcctgt tgtcgatata tacctggtgg
    55861 cttcaggtgc tgacacacaa tctgcggcta tggcattagc agagcgtctg cgtgatgaat
    55921 taccgggcgt gaaactgatg accaaccacg gcggcggcaa ctttaagaaa cagtttgccc
    55981 gtgctgataa atggggtgcc cgcgttgctg tggtgctggg tgagtctgaa gtggctaacg
    56041 gcacagcagt agtgaaggat ttgcgctctg gtgagcaaac tgcagttgcg caggatagcg
    56101 tagccgcgca tttgcgcacg ttactgggtt aaggaaggag aaggacagcg tggaaattta
    56161 cgagaacgaa aacgaccagg ttgaagcggt taaacgcttt tttgctgaaa atggcaaagc
    56221 actggctgtt ggggtgattt tgggcgttgg cgcactgatt ggctggcgct actggaatag
    56281 ccatcaggtt gattctgcac gctccgcttc tcttgcctat caaaatgcgg ttaccgcagt
    56341 gagcgaaggc aaaccggata gcatcccagc ggcggaaaaa tttgctgctg aaaataaaaa
    56401 tacttatggt gcgctggctt ctttggaact tgcgcagcaa tttgttgaca aaaatgaact
    56461 agagaaagct gccgcccagt tacaacaggg gctggcagac acgagcgatg aaaatctcaa
    56521 agccgtgata aatctgcgtc ttgctcgcgt tcaggtacag ctcaagcagg ctgatgccgc
    56581 gctaaaaacc cttgatgcta tcaaaggtga agggtgggct gccattgttg ccgacctgcg
    56641 tggtgaagca ttgctgagca aaggtgataa gcaaggtgcg cgtagtgcat gggaagcagg
    56701 cgtgaaaagc gatgttaccc cggcactgag cgaaatgatg cagatgaaaa ttaataattt
    56761 gtccatctga gagggacccg atgcaattgc gtaaattact gctgccagga ctgctttccg
    56821 ttaccctttt aagcggctgt tcgctgttta acagcgaaga agatgtggta aagatgtccc
    56881 cattgccaac cgttgaaaac cagtttacgc cgactactgc gtggagcact tccgttggta
    56941 gcggcattgg caacttctat tccaatcttc atccggcact ggcggacaac gttgtctatg
    57001 cagcggaccg cgctggttta gtaaaagcgc tgaatgcgga tgacggcaaa gaaatctggt
    57061 ctgtcaacct ggccgagaaa gatggctggt tctctaaaga tcctgcatta ctttctggcg
    57121 gcgtgaccgt gtctggtggg catgtctaca ttggcagcga aaaggcgcag gtttacgcgc
    57181 tgaataccag cgatggtact gtggcatggc aaactaaagt cgcgggtgaa gcactttcgc
    57241 gcccggtggt cagcgacggt ctggtgttaa tccacaccag taacggtcag ttacaagcgc
    57301 tgaacgaagc tgacggcgct gtcaaatgga cagttaacct cgatatgccg tcgctctctt
    57361 tgcgtggcga gtctgcgccg gcaacggctt ttggtgcggc cgtcgtgggt ggcgataatg
    57421 gtcgcgtcag cgcagtgctg atggaacagg gccagatgat ttggcagcag cgtatttccc
    57481 aggcgaccgg ttctaccgaa attgaccgtc tgagcgatgt tgacacgact cccgtcgttg
    57541 ttaacggcgt tgttttcgcg ctggcctata atggtaacct gacggcgctt gatctgcgca
    57601 gcggtcagat tatgtggaaa cgcgaactgg gttcggtgaa tgatttcatc gtcgacggca
    57661 accgcatcta tctggtcgat caaaatgacc gggtgatggc gttgaccatt gatggcggcg
    57721 ttacgctgtg gacacaaagc gatctgctgc atcgcctgct gacttctccg gtgctgtata
    57781 atggcaacct ggtggtaggt gacagtgaag gttatctgca ctggattaac gtcgaagatg
    57841 gtcgtttcgt tgcccagcaa aaagttgata gttccggttt ccagactgaa ccggttgctg
    57901 ctgacggcaa actgctgatc caggcaaaag acggaaccgt gtactctatt acacgttaat
    57961 cgtctctgtc gttcactttg aaaacggctc ctggacaggg gccgttttcc tgtttttaac
    58021 aacgacacga atatagcgtg cgttgtctga tgatttataa aaatgaggct ttaaacatgg
    58081 tacctgtggt cgcgcttgtc gggcgcccta acgtaggaaa atccacgtta tttaaccgtc
    58141 taactcgcac ccgagatgcg ctggttgcgg atttcccggg tctgactcgt gaccgtaagt
    58201 acggtcgtgc ggaaattgaa ggccgcgagt ttatctgcat tgataccggc gggattgatg
    58261 gcaccgaaga cggtgtagag acacgtatgg cggaacagtc gctgctggcg attgaagaag
    58321 cggacgttgt actgtttatg gtggatgcgc gcgcgggcct gatgccggcg gatgaagcga
    58381 ttgctaaaca tctgcgctcc cgtgaaaaac cgaccttcct ggtggcaaac aaaactgacg
    58441 gtctggatcc cgatcaggca gtggttgatt tctacgcgct tggtttaggt gaaatctacc
    58501 cgatcgccgc gtctcacggt cgtggcgtat taagtctgct ggagcacgtg ctgctgccgt
    58561 ggatggaaga tctcgcaccg caagaagaag tcgacgaaga cgccgaatac tgggcgcaat
    58621 ttgaagcgga agagaacggc gaagaagaag aggcagacga cttcgacccg caaagtctgc
    58681 cgatcaaact ggcgattgtg ggtcgtccga acgtaggtaa gtctacactc actaaccgta
    58741 ttcttggtga agagcgcgtt gttgtttacg acatgcctgg cacgacgcgt gacagtatct
    58801 acattccaat ggaacgcgac ggacgtgagt atgtgctcat tgacaccgct ggtgtacgta
    58861 aacgcggcaa aatcaccgat gcggtagaga aattctcggt aatcaaaacg ttgcaggcca
    58921 ttgaagatgc caacgtggtg atgttggtga ttgatgcgcg tgaaggtatt tccgatcagg
    58981 atctctcctt gctgggcttt attctcaata gtgggcgctc acttgtgatt gtagtgaaca
    59041 agtgggacgg cctgagccag gaagtgaaag agcaggtgaa agagacgctg gacttccgtc
    59101 tgggctttat cgattttgct cgtgtgcact ttatctctgc cttgcacggc agtggtgttg
    59161 gtaacttgtt tgaatcagta cgtgaagcgt atgacagctc cacccgtcgt gtggggacct
    59221 ctatgctgac gcgcatcatg acgatggctg ttgaagatca ccaaccaccg ctggtacgcg
    59281 gtcgtcgtgt gaagctgaaa tacgcccacg ccgggggtta taacccgccg attgtggtga
    59341 ttcacggcaa tcaggtgaaa gacctgcctg attcctacaa gcgttacctg atgaactact
    59401 tccgcaaatc gctggacgta atgggatcgc cgattcgtat tcagttcaaa gaaggggaaa
    59461 acccgtatgc gaacaagcgt aacaccctga cgccaaccca gatgcgtaaa cgtaagcgtc
    59521 tgatgaagca tatcaagaaa agtaagtaat cacttatctg gtttcccttt ccacacggag
    59581 agggaagccg aataacacgc gaggagtatt tatggcactc aactgtccaa cctgcaaaac
    59641 ccctctcgaa cgcaatggcg agactgctca ctgtgcaact tgcgacaaag actttaccgt
    59701 ccaggcactt tgccctgagt gtcatcaggc gttacaagtg ttaaaagctt gtggtgctgt
    59761 ggattatttc tgccaacatg ggcacggttt aatatctaaa aaacgcgttg aatttattat
    59821 tgcttaatat catgt
//
LOCUS       DAZFLD010000006        55796 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.006, whole genome shotgun sequence.
ACCESSION   DAZFLD010000006 DAZFLD010000000
VERSION     DAZFLD010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 55796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55796
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.006"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(255..1655)
                     /gene="asnS"
                     /locus_tag="ACHKBL_000308"
     CDS             complement(255..1655)
                     /gene="asnS"
                     /locus_tag="ACHKBL_000308"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HFO5817234.1"
                     /translation="MSVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDG
                     SCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKVVASPGQGQQFEIQASKVEVAGW
                     VEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWV
                     STPLITASDTEGAGEMFRVSTLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGET
                     YACALSKIYTFGPTFRAENSNTSRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKA
                     VLEERADDMKFFAERVDKDAVSRLERFIEADFAQVDYTDAVTILENCGRKFENPVYWG
                     VDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIG
                     GSQREERLDVLDERMLEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERLIAYVTGVQN
                     VRDVIPFPRTPRNASF"
     gene            complement(1824..3026)
                     /gene="pncB"
                     /locus_tag="ACHKBL_000309"
     CDS             complement(1824..3026)
                     /gene="pncB"
                     /locus_tag="ACHKBL_000309"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HFO5817235.1"
                     /translation="MTQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGD
                     DLLGIYADAIREQVQAMQYLRLQDDEYQWLSALPFFKADYLNWLREFRFNPEQVTVSN
                     DNGKLDIRLSGPWREVILWEVPLLAVISEMVHRYRSPQADVAQALDTLESKLADFSAL
                     TAGLDMSRFHLMDFGTRRRFSREVQETIVKRLQQESWFVGTSNYDLARRLSLTPMGTQ
                     AHEWFQAHQQISPDLANSQRAALAAWLEEYPDQLGIALTDCITMDAFLRDFGVEFASR
                     YQGLRHDSGDPVEWGEKAIAHYEKLGIDPQSKTLVFSDNLDLRKAVELYRHFSSRVQL
                     SFGIGTRLTCDIPQVKPLNIVIKLVECNGKPVAKLSDSPGKTICHDKAFVRALRKAFD
                     LPHIKKAS"
     gene            3292..5904
                     /gene="pepN"
                     /locus_tag="ACHKBL_000310"
     CDS             3292..5904
                     /gene="pepN"
                     /locus_tag="ACHKBL_000310"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HFO5817236.1"
                     /translation="MTQQPQAKYRHDYRAPDYQITDIDLTFDLDAQKTVVTAVSQAVR
                     HGASDVPLRLNGEDLKLVSVYINDEPWTAWKEEEGALVISNLPERFTLKIVNEISPAA
                     NTALEGLYQSGDALCTQCEAEGFRHITYYLDRPDVLARFTTKIIADKTKYPFLLSNGN
                     RVAQGELENGRHWVRWQDPFPKPCYLFALVAGDFDVLRDTFTTRSGREVALELYVDRG
                     NLDRAPWAMTSLKNSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKYV
                     LARTDTATDKDYLDIERVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSSDL
                     GSRAVNRINNVRTMRGLQFAEDASPMAHPIRPDMVIEMNNFYTLTVYEKGAEVIRMIH
                     TLLGEENFQKGMQLYFERHDGSAATCDDFVQAMEDASNVDLSHFRRWYSQSGTPVVTV
                     KDDYNPETEQYTLTISQRTPATPDQAEKQPLHIPFAIELYDNEGKVIPLQKGGHPVNS
                     VLNVTQAEQTFVFDNVYFQPVPALLCEFSAPVKLEYKWSDQQLTFLMRHARNDFSRWD
                     AAQSLLATYIKLNVARHQQGQLLSLPVHVADAFRAVLLDEKIDPALAAEILTLPSVNE
                     MAELFDIIDPIAIAEVREALTRTLATELADELLAIYNANYQSEYRVEHEDIAKRTLRN
                     ACLRFLAFGETHLADVLVSKQYHEANNMTDALAALSAAVAAQLPCRDALMQEYDDKWH
                     QDGLVMDKWFILQATSPAANVLETVRGLLQHRSFTMSNPNRIRSLIGAFAGSNPAAFH
                     AEDGSGYQFLVEMLTDLNSRNPQVASRLIEPLIRLKRYDAKRQEKMRAALEQLKGLEN
                     LSGDLYEKITKALA"
     gene            complement(6110..6877)
                     /gene="ssuB"
                     /locus_tag="ACHKBL_000311"
     CDS             complement(6110..6877)
                     /gene="ssuB"
                     /locus_tag="ACHKBL_000311"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonates ABC transporter ATP-binding
                     protein"
                     /protein_id="HFO5817237.1"
                     /translation="MNTARLNQGTPLLLNAVSKQYAENIVLNQLDLHIPAGQFVAVVG
                     RSGGGKSTLLRLLAGLETPTAGDVLAGTTPLAEIQEDTRMMFQDARLLPWKSVIDNVG
                     LGLKGQWRDAARRALAAVGLENRAGEWPAALSGGQKQRVALARALIHRPGLLLLDEPL
                     GALDALTRLEMQDLIVSLWQEHGFTVLLVTHDVSEAVAMADRVLLIEEGKIGLDLTVD
                     IPRPRRLGSVRLAELEAEVLQRVMQRGHSEQPIRRHG"
     gene            complement(6874..7665)
                     /gene="ssuC"
                     /locus_tag="ACHKBL_000312"
     CDS             complement(6874..7665)
                     /gene="ssuC"
                     /locus_tag="ACHKBL_000312"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter permease
                     SsuC"
                     /protein_id="HFO5817238.1"
                     /translation="MATPVKKWLLRVAPWFLPVGIVAVWQLASSVGWLSTRILPSPEG
                     VVMAFWTLSASGELWQHLAISSWRALIGFSIGGSLGLILGLISGLSRWGERLLDTSIQ
                     MLRNVPHLALIPLVILWFGIDESAKIFLVALGTLFPIYINTWHGIRNIDRGLVEMARS
                     YGLSGIPLFIHVILPGALPSIMVGVRFALGLMWLTLIVAETISANSGIGYLAMNAREF
                     LQTDVVVVAIILYALLGKLADVSAQLLERLWLRWNPAYHLKEATV"
     gene            complement(7677..8822)
                     /gene="ssuD"
                     /locus_tag="ACHKBL_000313"
     CDS             complement(7677..8822)
                     /gene="ssuD"
                     /locus_tag="ACHKBL_000313"
                     /EC_number="1.14.14.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008726 - alkanesulfonate monooxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMNH2-dependent alkanesulfonate monooxygenase"
                     /protein_id="HFO5817239.1"
                     /translation="MSLNMFWFLPTHGDGHYLGTEEGSRPVDHGYLQQIAQAADRLGY
                     TGVLIPTGRSCEDAWLVAASMIPVTQRLKFLVALRPSVTSPTVAARQAATLDRLSNGR
                     ALFNLVTGSDPQELAGDGVFLDHSERYEASAEFTQVWRRLLLGETVNFNGKHIHVRGA
                     KLLFPPIQQPYPPLYFGGSSDVAQELAAEQVDLYLTWGEPPELVKEKIEQVRAKAAAH
                     GRKIRFGIRLHVIVRETNDEAWQAAERLISHLDDETIAKAQAAFARTDSVGQQRMAAL
                     HNGKRDNLEISPNLWAGVGLVRGGAGTALVGDGPTVAARINEYAALGIDSFVLSGYPH
                     LEEAYRVGELLFPHLDVAIPEIPQPQPLNPQGEAVANDFIPRNVAQS"
     gene            complement(8819..9778)
                     /gene="ssuA"
                     /locus_tag="ACHKBL_000314"
     CDS             complement(8819..9778)
                     /gene="ssuA"
                     /locus_tag="ACHKBL_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter
                     substrate-binding protein SsuA"
                     /protein_id="HFO5817240.1"
                     /translation="MRKIIKLALAGLLSVSTLAVAAESSPEALRIGYQKGSIGMVLAK
                     SHQLLEKRYPQTKISWVEFPAGPQMLEALNVGSIDLGSTGDIPPIFAQAAGADLVYVG
                     VEPPKPKAEVILVAENSPIKTVADLKGHKVAFQKGSSSHNLLLRALRQAGLKFTDIQP
                     TYLTPADARAAFQQGNVDAWAIWDPYYSAALLQGGVRVLKDGTDLNQTGSFYLAARPY
                     AEKNGAFIQGVLTTFSEADALTRSQREQSIALLAKTMGLPAPVIASYLDHRPPTTIKP
                     LSAEVAALQQQTADLFYENRLVPKKVDIRQRIWQPTQLEGKQL"
     gene            complement(9771..10346)
                     /gene="ssuE"
                     /locus_tag="ACHKBL_000315"
     CDS             complement(9771..10346)
                     /gene="ssuE"
                     /locus_tag="ACHKBL_000315"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_process: GO:0046306 - alkanesulfonate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFO5817241.1"
                     /translation="MRVITLAGSPRFPSRSSSLLEYAREKLNGLDVEVYHWNLQNFVP
                     EDLLYARFDSPALKTFTEQLQQADGLIVATPVYKAAYSGALKTLLDLLPERALQGKVV
                     LPLATGGTVAHLLAVDYALKPVLSTLKAQEILHGVFADDSQVIDYQHKPQFTPNLQTR
                     LDTALETFWQALHRRDVQVPDLLSLRGNAHA"
     gene            10583..11593
                     /gene="pyrD"
                     /locus_tag="ACHKBL_000316"
     CDS             10583..11593
                     /gene="pyrD"
                     /locus_tag="ACHKBL_000316"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HFO5817242.1"
                     /translation="MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPA
                     KPVNCMGLTFKNPLGLAAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFR
                     LVDAEGLINRMGFNNLGVDNLVENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICME
                     KIYAYAGYIAINISSPNTPGLRTLQYGEALDDLLTAIKNKQNDLQAMHHKYVPIAVKI
                     APDLSEEELIQVADSLVRHNIDGVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKST
                     EIIRRLSQELNGRLPIIGVGGIDSVIAAREKIAAGASLVQIYSGFIFKGPPLIKEIVT
                     HI"
     gene            11767..12309
                     /gene="zapC"
                     /locus_tag="ACHKBL_000317"
     CDS             11767..12309
                     /gene="zapC"
                     /locus_tag="ACHKBL_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapC"
                     /protein_id="HFO5817243.1"
                     /translation="MRIKPDDNWRWYYDEEHDRMMLDLANGMLFRSRFARKMLTPDAF
                     SPAGFCVDDAALYFSFEEKCRDFNLSKEQKAELVLNALVAIRYLKPQMPKSWHFVSHG
                     EMWVPMPGDAACVWLSDTHEQVNLLVVESGENAALCLLAQPCVVIAGRAMQLGDAIKI
                     MNDRLKPQVNVDSFNLEQAV"
     gene            complement(12306..13415)
                     /gene="ycbX"
                     /locus_tag="ACHKBL_000318"
     CDS             complement(12306..13415)
                     /gene="ycbX"
                     /locus_tag="ACHKBL_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein YcbX"
                     /protein_id="HFO5817244.1"
                     /translation="MATLTRLFIHPVKSMRGIGLTHALADVSGLAFDRIFMITEPDGT
                     FITARQFPQMVRFTPSPVHDGLHLTAPDGSSAYVRFADFATQDAPTEVWGTHFTARIA
                     PDAINKWLSGFFSREVQLRWVGPQMTRRVKRHNTVPLSFADGYPYLLANEASLRDLQQ
                     RCPASVKMEQFRPNLVVSGASAWEEDSWKVIRIGDVVFDVVKPCSRCIFTTVSPEKGQ
                     KHPAGEPLKTLQSFRTAQDNGDVDFGQNLIARNSGVIRVGDEVEVLATAPAKIYGAAA
                     ADDTVNITQQPDANVDIDWQGQAFRGNNQQVLLEQLENQGIRIPYSCRAGICGSCRVQ
                     LLEGEVTPLKKSAMGDDGTILCCSCVPKTALKLTR"
     gene            13659..15767
                     /gene="rlmKL"
                     /locus_tag="ACHKBL_000319"
     CDS             13659..15767
                     /gene="rlmKL"
                     /locus_tag="ACHKBL_000319"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HFO5817245.1"
                     /translation="MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLV
                     YQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAINWTEMFNPGATFAVHFSGLNDTI
                     RNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLH
                     LRGYRDRAGIAPIKETLAAAIVMRSGWQPGTPLLDPMCGSGTLLIEAAMLATDRAPGL
                     HRGRWGFSGWAQHDEAIWQEVKAEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNAR
                     LAGIGELITFEVKDVAQLTNPLPKGPYGTVLSNPPYGERLDSEPALIALHSLLGRIMK
                     NQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPVMAA
                     EDYANRLRKNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYADWVVVQEYAPPKTI
                     DAHKARQRLFDIIAATISVLGIAPNKLVLKTRERQKGKNQYQKLGEKGEFLEVTEYNA
                     HLWVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGGARSTTT
                     VDMSRTYLEWAERNLRLNGLTGRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKR
                     MEDAFDVQRDHLALMKDLKRLLRAGGTIMFSNNKRGFRMDLDGLAKLGLKAQEITQKT
                     LSQDFARNRQIHNCWLITAA"
     gene            15779..17686
                     /gene="uup"
                     /locus_tag="ACHKBL_000320"
     CDS             15779..17686
                     /gene="uup"
                     /locus_tag="ACHKBL_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO5817246.1"
                     /translation="MSLISMHGAWLSFSDAPLLDNAELHIEDNERVCLVGRNGAGKST
                     LMKILNREQGLDDGRIIYEQDLIVARLQQDPPRNVEGSVYDFVAEGIEEQAEYLKRYH
                     DISRLVMNDPSEKNLNELAKVQEQLDHHNLWQLENRINEVLAQLGLDPNVALSSLSGG
                     WLRKAALGRALVSNPRVLLLDEPTNHLDIETIDWLEGFLKTFNGTIIFISHDRSFIRN
                     MATRIVDLDRGKLVTYPGNYDQYLLEKEEALRVEELQNAEFDRKLAQEEVWIRQGIKA
                     RRTRNEGRVRALKAMRRERGERREVMGTAKMQVEEASRSGKIVFEMEDVCYQVDGKQL
                     VKDFSAQVLRGDKIALIGPNGCGKTTLLKLMLGQLQADSGRIHVGTKLEVAYFDQHRA
                     ELDPDKTVMDNLAEGKQEVMVNGKPRHVLGYLQDFLFHPKRAMTPVRALSGGERNRLL
                     LARLFLKPSNLLILDEPTNDLDVETLELLEELIDSYQGTVLLVSHDRQFVDNTVTECW
                     IFEGGGKIGRYVGGYHDARGQQEQYVALKQPAVKKIEEAAAPKAETVKRSSSKLSYKL
                     QRELEQLPQLLEDLEAKLEALQTQVADASFFSQPHEQTQKVLADMAAAEQELEQAFER
                     WEYLEALKNGG"
     gene            17816..19069
                     /gene="pqiA"
                     /locus_tag="ACHKBL_000321"
     CDS             17816..19069
                     /gene="pqiA"
                     /locus_tag="ACHKBL_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiA"
                     /protein_id="HFO5817247.1"
                     /translation="MCEHHHAAKHILCSQCDMLVALPRLEHGQKAACPRCGTTLTVAW
                     DAPRQRPTAYALAALFMLLLSNLFPFVNMNVAGVTSEITLLEIPGVLFSEDYASLGTF
                     FLLFVQLVPAFCLITILLLVNRAELPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFV
                     KLMAYGSIGVGSSFLPWCLFCVLQLRAFQCVDRRWLWDDIAPMPELRQPLKPGVTGIR
                     QGLRSCSCCTAILPADDPVCPRCGTKGYVRRRNSLQWTLALLVTSIMLYLPANILPIM
                     VTDLLGSKMPSTILAGVILLWSEGSYPVAAVIFLASIMVPTLKMIAIAWLCWDAKGHG
                     KRDSERMHLIYEVVEFVGRWSMIDVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIM
                     TMFSAMTFDPRLSWDRQPESEHEES"
     gene            19074..20714
                     /gene="pqiB"
                     /locus_tag="ACHKBL_000322"
     CDS             19074..20714
                     /gene="pqiB"
                     /locus_tag="ACHKBL_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HFO5817248.1"
                     /translation="MESNNGEAKIQKVKNWSPVWIFPIVTALIGAWVLFYHYSHQGPE
                     VTLITANAEGIEGGKTTIKSRSVDVGVVESATLADDLTHVEIKARLNSGMEKLLHKDT
                     VFWVVKPQIGREGISGLGTLLSGVYIELQPGAKGSKMDKYDLLDSPPLAPPDAKGIRV
                     ILDSKKAGQLSPGDPVLFRGYRVGSVETSTFDTQKRNISYQLFINAPYDRLVTSNVRF
                     WKDSGIAVDLTSAGMRVEMGSLTTLLSGGVSFDVPEGLDLGQPVAPKTAFVLYDDQKS
                     IQDSLYTDHIDYLMFFKDSVRGLQPGAPVEFRGIRLGTVSKVPFFAPNMRQTFNDDYR
                     IPVLIRIEPERLKMQLGENADVVEHLGELLKRGLRGSLKTGNLVTGALYVDLDFYPNT
                     PAITGIREFNGYQIIPTVSGGLAQIQQRLMEALDKINKLPLNPMIEQATSTLSESQRT
                     MKNLQTTLDSMNKILASQSMQQLPTDMQSTLRELNRSMQGFQPGSAAYNKMVADMQRL
                     DQVLRELQPVLKTLNEKSNALVFEAKDKKDPEPKRAKQ"
     gene            20711..21274
                     /gene="pqiC"
                     /locus_tag="ACHKBL_000323"
     CDS             20711..21274
                     /gene="pqiC"
                     /locus_tag="ACHKBL_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309063.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HFO5817249.1"
                     /translation="MKKWLVTIAALWLAGCSSGEINKNYYQLPVVQSGTQSTASQGNR
                     LLWVEQVAVPDYLAGNGVVYQTSDVKYVIANNNLWASPLDQQLRNTLVANLSTQLPGW
                     VVASQPLGSAQDTLNVTVTEFNGRYDGKVIVSGEWLLNHQGQLIKRPFRLEGVQTQDG
                     YDEMVKVLASVWSQEAASIAQEIKRLP"
     gene            21530..21697
                     /gene="rmf"
                     /locus_tag="ACHKBL_000324"
     CDS             21530..21697
                     /gene="rmf"
                     /locus_tag="ACHKBL_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0141014 - ribosome hibernation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HFO5817250.1"
                     /translation="MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLNQRSQWLGGW
                     REAMADRVVMA"
     gene            complement(21767..22285)
                     /gene="fabA"
                     /locus_tag="ACHKBL_000325"
     CDS             complement(21767..22285)
                     /gene="fabA"
                     /locus_tag="ACHKBL_000325"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014915875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008693 -
                     (3R)-3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HFO5817251.1"
                     /translation="MVDKRESYTKEDLLASGRGELFGAKGPQLPAPNMLMMDRVVKMT
                     ETGGNFDKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEG
                     KGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLADGEVLVDGRLIYTASD
                     LKVGLFQDTSAF"
     gene            complement(22354..24114)
                     /gene="ycbZ"
                     /locus_tag="ACHKBL_000326"
     CDS             complement(22354..24114)
                     /gene="ycbZ"
                     /locus_tag="ACHKBL_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFO5817252.1"
                     /translation="MTITKLAWRDLVPDTDSYQEIFAQPHLIDENDPLFSDTQPRLQF
                     ALEQLLHTRASSSFMLAKAPEESEYLNLIADAARALQSDAGQLVGGHYEVSGHTIRLR
                     NAVSADDNFATLTQVVAADWVEAEQLFGCLRQFNGDITLQPGLVHQANGGILIISLRT
                     LLAQPLLWMRLKNIVNRERFDWVAFDESRPLPVSVPSMPLKLKVILVGERESLADFQE
                     MEPELSEQAIYSEFEDTLQIVDAESVSQWCRWVTLTARHNHLPAPGADAWPVLIREAA
                     RYTGEQETLPLSPQWILRQCKEVASLCDGDTFSGEQLNLMLQQREWREGFLAERMQDE
                     ILQEQILIETEGERIGQINALSVIEFPGHPRAFGEPSRISCVVHIGDGEFTDIERKAE
                     LGGNIHAKGMMIMQAFLMSELQLEQQIPFSASLTFEQSYSEVDGDSASMAELCALISA
                     LADVPVNQSIAITGSVDQFGRAQPVGGLNEKIEGFFAICQQRELTGKQGVIIPTANIR
                     HLSLHSELVKAVEEGKFTIWAVDDVTDALPLLLNLVWDGEGQTTLMQTIQERIAQASQ
                     QEGRHRFPWPLRWLNWFIPN"
     gene            24300..24752
                     /gene="matP"
                     /locus_tag="ACHKBL_000327"
     CDS             24300..24752
                     /gene="matP"
                     /locus_tag="ACHKBL_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HFO5817253.1"
                     /translation="MKYQQLENLESGWKWKYLVKKHREGELITRYIEASAAQEAVDVL
                     LSLENEPVLVNGWIDKHMNPELVNRMKQTIRARRKRHFNAEHQHTRKKSIDLEFIVWQ
                     RLAGLAQRRGKTLSETIVQLIEDAENKEKYANKMSSLKQDLQALLGKE"
     gene            complement(24828..25880)
                     /gene="ompA"
                     /locus_tag="ACHKBL_000328"
     CDS             complement(24828..25880)
                     /gene="ompA"
                     /locus_tag="ACHKBL_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HFO5817254.1"
                     /translation="MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGF
                     IPNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGDNINGAYKAQGVQLTA
                     KLGYPITDDLDVYTRLGGMVWRADTKSNVPGGASTKDHDTGVSPVFAGGVEYAITPEI
                     ATRLEYQWTNNIGDAHTIGTRPDNGMLSLGVSYRFGQGEAAPVVAPAPAPAPEVQTKH
                     FTLKSDVLFTFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQA
                     LSERRAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRV
                     EIEVKGIKDVVTQPQA"
     gene            complement(26236..26745)
                     /gene="sulA"
                     /locus_tag="ACHKBL_000329"
     CDS             complement(26236..26745)
                     /gene="sulA"
                     /locus_tag="ACHKBL_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0051782 - negative regulation of cell
                     division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS-induced cell division inhibitor SulA"
                     /protein_id="HFO5817255.1"
                     /translation="MYTSGYAHRASSFSSAASKIARVSTENTTAGLISEVVYREDQPM
                     MTQLLLLPLLQQLGQQSRWQLWLTPQQKLSREWVQASGLPLTKVMQISQLSPCHTVES
                     MVRALRTGNYSVVIGWLADDLTEEEHAELVDAANEGNAMGFIMRPVSASSHATRQLSG
                     LKIHSNLYH"
     gene            26963..27592
                     /gene="sxy"
                     /locus_tag="ACHKBL_000330"
     CDS             26963..27592
                     /gene="sxy"
                     /locus_tag="ACHKBL_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRP-S regulon transcriptional coactivator Sxy"
                     /protein_id="HFO5817256.1"
                     /translation="MKSLSYKRIYKSQEYLATLGTIEYRSLFGSYSLTVDDTVFAMVS
                     DGELYLRACEQSAQYCVKHPPVWLTYKKCGRSVTLNYYRVDESLWRNQLKLVRLSKYS
                     LDAALKEKSTRNTRERLKDLPNMSFHLEAILGEVGIKDVRALRILGAKMCWLRLRQQN
                     SLVTEKILFMLEGAIIGIHEAALPVARRQELAEWADSLTPKQEFPAELE"
     gene            complement(27555..29717)
                     /gene="yccS"
                     /locus_tag="ACHKBL_000331"
     CDS             complement(27555..29717)
                     /gene="yccS"
                     /locus_tag="ACHKBL_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309071.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YccS family putative transporter"
                     /protein_id="HFO5817257.1"
                     /translation="MAFMLSPLLKRYTWNSAWLYYARIFIALCGTTAFPWWLGDVKLT
                     IPLTLGMVAAALTDLDDRLAGRLRNLIITLFCFFIASASVELLFPWPWLFAIGLTLST
                     SGFILLGGLGQRYATIAFGALLIAIYTMLGTSLYEHWYQQPMYLLAGAVWYNVLTLIG
                     HLLFPVRPLQDNLARCYEQLARYLELKSRMFDPDIEDESQAPLYDLALANGQLMATLN
                     QTKLSLLTRLRGDRGQRGTRRTLHYYFVAQDIHERASSSHIQYQTLREHFRHSDVLFR
                     FQRLMSMQGQACQQLSRCILLRQPYQHDPHFERAFTHIDAALERMRDNGAPADLLKTL
                     GFLLNNLRAIDAQLATIESEQAQALPHNNDENELADDSPHGLSDIWLRLSRHFTPESA
                     LFRHAVRMSLVLCFGYAIIQITGMHHGYWILLTSLFVCQPNYNATRHRLKLRIIGTLV
                     GIAIGIPVLWFVPSLEGQLVLLVITGVLFFAFRNVQYAHATMFITLLVLLCFNLLGEG
                     FEVALPRVIDTLIGCAIAWAAVSYIWPDWKFRNLPRMLERATEANCRYLDAILEQYHQ
                     GRDNRLAYRIARRDAHNRDAELASVVSNMSSEPNVTPQIREAAFRLLCLNHTFTSYIS
                     ALGAHREQLTNPEILAFLDDAVCYVDDALHHQPADEERVNQALAGLKQRMQQLEPRAD
                     SKEPLVVQQVGLLIALLPEIGRLQRQITQVPQETPVSA"
     gene            complement(29727..30173)
                     /gene="yccF"
                     /locus_tag="ACHKBL_000332"
     CDS             complement(29727..30173)
                     /gene="yccF"
                     /locus_tag="ACHKBL_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HFO5817258.1"
                     /translation="MRTVLNILNFVLGGFATTLGWLLATLVSIVLIFTLPLTRSCWEI
                     TKLSLVPYGNEAIHVDELNPAGKNVLLNTGGTVLNIFWLIFFGWWLCLMHIATGIAQC
                     ISIIGIPVGIANFKIAAIALWPVGRRVVSVETAQAAREANARRRFE"
     gene            30296..32350
                     /gene="helD"
                     /locus_tag="ACHKBL_000333"
     CDS             30296..32350
                     /gene="helD"
                     /locus_tag="ACHKBL_000333"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase IV"
                     /protein_id="HFO5817259.1"
                     /translation="MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLL
                     AIHCKRGLVWGELEFVLPGEKVVRLHGTEWGETQRFYHHLDAHWQRWSSEMSEIASGV
                     LRQQLDLIATRTGENKWLTREQTSGVQQQIRQALSALPLPVNRLEEFDNCREAWRKCQ
                     AWLKDIESARLQHNQAYTEAMLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLAGA
                     GSGKTSVLVARAGWLLARGEASPEQILLLAFGRKAAEEMDERIRERLHTEDITARTFH
                     ALALHIIQQGSKKVPIVSKLENDTAARHELFIAEWRKQCSEKKAQAKGWRQWLTEEMQ
                     WSVPEGNFWDDEKLQRRLASRLDRWVSLMRMHGGAQAEMIASAPEVIRDLFSKRIKLM
                     APLLKAWKGALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISPQRAALL
                     AALRKQNSQTTLFAVGDDWQAIYRFSGAQMSLTTAFHENFGEGDRCDLDTTYRFNSRI
                     GEVANRFIQQNPGQLKKPLNSLTNGDKKAVTLLDESHLDALLDKLSGYAKPEERILIL
                     ARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVIIVGLQEGSDGFPAAARES
                     IMEEALLPSVEDFPDAEERRLMYVALTRARHRVWALFNKENPSPFVEILKNLDVPVAR
                     KP"
     gene            complement(32382..32840)
                     /gene="mgsA"
                     /locus_tag="ACHKBL_000334"
     CDS             complement(32382..32840)
                     /gene="mgsA"
                     /locus_tag="ACHKBL_000334"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HFO5817260.1"
                     /translation="MELTTRTLPARKHIALVAHDHCKQMLMSWVERHQPLLEQHVLYA
                     TGTTGNLISRATGMNVNAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPHDPDV
                     KALLRLATVWNIPVATNVATADFIIQSPHFNDAVDILIPDYQRYLADRLK"
     gene            complement(32936..33598)
                     /gene="yccT"
                     /locus_tag="ACHKBL_000335"
     CDS             complement(32936..33598)
                     /gene="yccT"
                     /locus_tag="ACHKBL_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HFO5817261.1"
                     /translation="MKTGIVTTLIALCLPVSVFATTLRLSTDVDLLVLDGKKVSSSLL
                     RGADSIELDNGPHQLVFRVEKTIHLSNSEERLYISPPLVVSFNTQLINQVNFRLPRLE
                     NEREANHFDAAPRLELLDGDATPIPVKLDILAITSTAKTIDYEVEVERYNKSAKRASL
                     PQFATMMADDSTLLSGVSELDAIPPQSQVLTEQRLKYWFKLADPQTRNTFLQWAEKQP
                     SS"
     gene            33771..34184
                     /gene="yccU"
                     /locus_tag="ACHKBL_000336"
     CDS             33771..34184
                     /gene="yccU"
                     /locus_tag="ACHKBL_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HFO5817262.1"
                     /translation="MKETDIAGILTSTHTIALVGASDKPDRPSYRVMKYLLDQGYHVI
                     PVSPKVAGKTLLGQQGYGTLAEVPEKVDMVDVFRNSEAAWGVAQEAIAIGAKTLWMQL
                     GVINEQAAVLARDAGLNVVMDRCPAIEIPRLGLAK"
     gene            complement(34229..34546)
                     /gene="hspQ"
                     /locus_tag="ACHKBL_000337"
     CDS             complement(34229..34546)
                     /gene="hspQ"
                     /locus_tag="ACHKBL_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HFO5817263.1"
                     /translation="MIASKFGIGQQVRHSLLGYLGVVVDIDPVYSLSEPSPDELAVND
                     ELRAAPWYHVVMEDDNGLPVHTYLAEAQLSSELQDEHPEQPSMDELAQTIRKQLQAPR
                     LRN"
     gene            complement(34604..35794)
                     /gene="rlmI"
                     /locus_tag="ACHKBL_000338"
     CDS             complement(34604..35794)
                     /gene="rlmI"
                     /locus_tag="ACHKBL_000338"
                     /EC_number="2.1.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
                     /protein_id="HFO5817264.1"
                     /translation="MSVRLVLAKGREKSLLRRHPWVFSGAVARMEGKASLGETIDIVD
                     HQGKWLARGAYSPASQIRARVWTFDPNESIDIAFFTRRLQQAQKWRDWLAQKDGLDSY
                     RLIAGESDGLPGITIDRFGNFLVLQLLSAGAEYQRAALISALQTLYPECAIYDRSDVA
                     VRKKEGMELTQGPITGELPPALLPIEEHGMKLLVNIQHGHKTGYYLDQRDSRLATRRY
                     VENKRVLNCFSYTGGFAVSALMGGCSQVVSVDTSQEALDIARQNVELNKLDLSKAEFV
                     RDDVFKLLRTYRDRGEKFDVIVMDPPKFVENKSQLMGACRGYKDINMLAIQLLNEGGI
                     LLTFSCSGLMTSDLFQKIIADAAIDAGRDVQFIEQFRQAADHPVIATYPEGLYLKGFA
                     CRVM"
     gene            35889..36167
                     /gene="yccX"
                     /locus_tag="ACHKBL_000339"
     CDS             35889..36167
                     /gene="yccX"
                     /locus_tag="ACHKBL_000339"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000048233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HFO5817265.1"
                     /translation="MSKVCIIAWVYGRVQGVGFRYTTQYEAKKLGLAGYAKNLDDGSV
                     EVVACGEEGQVEKLMQWLKSGGPRSARVERVLSEPHHPSGELTDFRIH"
     gene            complement(36164..36493)
                     /gene="tusE"
                     /locus_tag="ACHKBL_000340"
     CDS             complement(36164..36493)
                     /gene="tusE"
                     /locus_tag="ACHKBL_000340"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusE"
                     /protein_id="HFO5817266.1"
                     /translation="MLIFEGKEIETDTEGYLKESSQWSEPLAVVIAENEGITLSPEHW
                     EVVRFVRNFYLEFNTSPAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGL
                     PKPVKCI"
     gene            complement(36584..37243)
                     /gene="yccA"
                     /locus_tag="ACHKBL_000341"
     CDS             complement(36584..37243)
                     /gene="yccA"
                     /locus_tag="ACHKBL_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease modulator YccA"
                     /protein_id="HFO5817267.1"
                     /translation="MDRIVSSSHDRTSLLSTHKVLRNTYFLLSLTLAFSAITATASTV
                     LMLPSPGLILTLVGMYGLMFLTYKTANKPTGIISAFAFTGFLGYILGPILNTYLSAGM
                     GDVIAMALGGTALVFFCCSAYVLTTRKDMSFLGGMLMAGIVVVLIGMVANIFLQLPAL
                     HLAISAVFILISSGAILFETSNIIHGGETNYIRATVSLYVSLYNIFVSLLSILGFASR
                     D"
     gene            complement(37452..37539)
                     /locus_tag="ACHKBL_000342"
     tRNA            complement(37452..37539)
                     /locus_tag="ACHKBL_000342"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(37503..37505),aa:Ser,seq:tga)
     gene            37966..39084
                     /gene="hyaA"
                     /locus_tag="ACHKBL_000343"
     CDS             37966..39084
                     /gene="hyaA"
                     /locus_tag="ACHKBL_000343"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 small subunit"
                     /protein_id="HFO5817268.1"
                     /translation="MNNEETFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAW
                     ALENKPRIPVVWIHGLECTCCTESFIRSAHPLAKDVILSLISLDYDDTLMAAAGTQAE
                     EVFEDIITQYNGKYILAVEGNPPLGEQGMFCISSGRPFIEKLKRAAAGASAIIAWGTC
                     ASWGCVQAARPNPTQATPIDKVITDKPIIKVPGCPPIPDVMSAIITYMVTFDRLPDVD
                     KMGRPLMFYGQRIHDKCYRRAHFDAGEFVQSWDDDAARKGYCLYKMGCKGPTTYNACS
                     STRWNDGVSFPIQSGHGCLGCAENGFWDRGSFYSRVVDIPQMGTHSTADTVGLTALGV
                     VAAAVGVHAVASAVDQRRRHNQQPTETEHQPGNEDKQA"
     gene            39081..40874
                     /gene="hyaB"
                     /locus_tag="ACHKBL_000344"
     CDS             39081..40874
                     /gene="hyaB"
                     /locus_tag="ACHKBL_000344"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase large subunit"
                     /protein_id="HFO5817269.1"
                     /translation="MSTQYETQGYTINNAGRRLVVDPITRIEGHMRCEVNINDQNVIT
                     NAVSCGTMFRGLEIILQGRDPRDAWAFVERICGVCTGVHALASVYAIEDAIGIKVPDN
                     ANIIRNIMLATLWCHDHLVHFYQLAGMDWIDVLDALKADPRKTSELAQSLSSWPKSSP
                     GYFFDVQNRLKKFVEGGQLGIFRNGYWGHPQYKLPPEANLMGFAHYLEALDFQREIVK
                     IHAVFGGKNPHPNWIVGGMPCAINIDESGAVGAVNMERLNLVQSIITRTADFINNVMI
                     PDALAIGQFNKPWSEIGTGLSDKCVLSYGAFPDIANDFGEKSLLMPGGAVINGDFNNV
                     LPVDLVDPQQVQEFVDHAWYRYPNDQVGRHPFDGITDPWYNPGDVKGSDTNIQQLNEQ
                     ERYSWIKAPRWRGNAMEVGPLARTLIAYHKGDAATVESVDRMMSALNLPLSGIQSTLG
                     RILCRAHEAQWAAGKLQYFFDKLMTNLKNGNLATASTEKWEPATWPTECRGVGFTEAP
                     RGALGHWAAIRDGKIDLYQCVVPTTWNASPRDPKGQIGAYEAALMNTKMAIPEQPLEI
                     LRTLHSFDPCLACSTHVLSDDGSELISVQVR"
     gene            40893..41600
                     /gene="hyaC"
                     /locus_tag="ACHKBL_000345"
     CDS             40893..41600
                     /gene="hyaC"
                     /locus_tag="ACHKBL_000345"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase b-type cytochrome subunit"
                     /protein_id="HFO5817270.1"
                     /translation="MQQKSDNVVSHYVFEAPVRIWHWLTVLCMAVLMVTGYFIGKPLP
                     SVSGEATYLFYMGYIRLIHFSAGMVFTVVLLMRIYWAFVGNRYSRELFIVPVWRKSWW
                     QGVWYEIRWYLFLAKRPSADIGHNPIAQAAMFGYFLMSVFMIITGFALYSEHSQYAIF
                     APFRYVVEFFYWTGGNSMDVHSWHRLGMWLIGAFVIGHVYMALREDIMSDDTVISTMV
                     NGYRSHKFGKISNKERS"
     gene            41597..42184
                     /gene="hyaD"
                     /locus_tag="ACHKBL_000346"
     CDS             41597..42184
                     /gene="hyaD"
                     /locus_tag="ACHKBL_000346"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 maturation protease"
                     /protein_id="HFO5817271.1"
                     /translation="MSEQRVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEYVEIVDG
                     GTQGLNLLGYVESASHLLILDAIDYGLEPGTLRTYAGERIPAYLSAKKMSLHQNSFSE
                     VLALADIRGHLPAHIALVGLQPAMLDDYGGSLSELAREQLPAAEQSALAQLAAWGIVP
                     QPAKESRCLNYDCLSMENYEGVRLRQYRMRLEEQG"
     gene            42181..42579
                     /gene="hyaE"
                     /locus_tag="ACHKBL_000347"
     CDS             42181..42579
                     /gene="hyaE"
                     /locus_tag="ACHKBL_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-1 operon protein HyaE"
                     /protein_id="HFO5817272.1"
                     /translation="MSNDTPFNALWQRMLARGWTPVSECRLDDWLTQAPDGVVLLSSD
                     PKRTPEVSDNPVMIGELLREFPDYTWQVAIADLEQSEAIGDRFGVFRFPATLVFTGGS
                     YRGVLNGIHPWAELINLMRGLVEPQQERAS"
     gene            42576..43433
                     /gene="hyaF"
                     /locus_tag="ACHKBL_000348"
     CDS             42576..43433
                     /gene="hyaF"
                     /locus_tag="ACHKBL_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HFO5817273.1"
                     /translation="MSETFFHLLGPGTQPNDDSFSMNPLPITCQVNDEPSMAALEQCA
                     HSPQVIALLNELQQQISERQPPLGEVLAVDLLNLNADDRHFINTLLGEGEVSVRIQQA
                     DDSESEIQEAIFCGLWRVRRRRGDKLLEDKLEAGCAPLALWQAATQNVLPTDSLLPPP
                     IDGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIG
                     YGESYINATGLRHVWHLRCTDTLKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVC
                     QWLAEGAPT"
     gene            43567..45111
                     /gene="appC"
                     /locus_tag="ACHKBL_000349"
     CDS             43567..45111
                     /gene="appC"
                     /locus_tag="ACHKBL_000349"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit 1"
                     /protein_id="HFO5817274.1"
                     /translation="MWDVIDLSRWQFALTALYHFLFVPLTLGLIFLLAIMETIYVVTG
                     KTIYRDMTRFWGKLFGINFALGVATGLTMEFQFGTNWSFYSNYVGDIFGAPLAMEALM
                     AFFLESTFVGLFFFGWQRLNKYQHLLVTWLVAFGSNLSALWILNANGWMQYPTGAHFD
                     IDTLRMEMTSFSELVFNPVSQVKFVHTVMAGYVTGAMFIMAISAWYLLRGRERDVALR
                     SFAIGSVFGTLAIIGTLQLGDSSAYEVAQVQPVKLAAMEGEWQTEPAPAPFHVVAWPE
                     QDQERNAFAIKIPALLGILATHSLDKPVPGLKNLMAETYPRLQRGRMAWLLMQEISQG
                     NREPHVLQAFRELEGDLGYGMLLSRYAPDMNHVTAAQYQAAMRGAIPQVAPVFWSFRI
                     MVGCGSLLLLVMLIALVQTLRGKIDQHRWVLKMALWSLPLPWIAIEAGWFMTEFGRQP
                     WAIQDILPTYSAHSALTTGQLAFSLIMIVGLYTLFLIAEVYLMQKYARLGPSAMQSEQ
                     PTQQQG"
     gene            45123..46259
                     /gene="appB"
                     /locus_tag="ACHKBL_000350"
     CDS             45123..46259
                     /gene="appB"
                     /locus_tag="ACHKBL_000350"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HFO5817275.1"
                     /translation="MFDYETLRFIWWLLIGVILVVFMISDGFDMGIGCLLPLVARNDD
                     ERRIVINSVGAHWEGNQVWLILAGGALFAAWPRVYAAAFSGFYVAMILVLCSLFFRPL
                     AFDYRGKIADARWRKMWDAGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGS
                     FWQLLTPFPLLCGLLSLGMVILQGGVWLQLKTVGVIHLRSQLATKRAALLVMLCFLLA
                     GYWLWVGIDGFVLLAQDANGPSNPLMKLVAVLPGAWMNNFVESPVLWIFPLLGFFCPL
                     LTVMAIYRGRPGWGFLMASLMQFGVIFTAGITLFPFVMPSSVSPISSLTLWDSTSSQL
                     TLSIMLVIVLIFLPIVLLYTLWSYYKMWGRMTTETLRRNENELY"
     gene            46272..46364
                     /gene="cbdX"
                     /locus_tag="ACHKBL_000351"
     CDS             46272..46364
                     /gene="cbdX"
                     /locus_tag="ACHKBL_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit CbdX"
                     /protein_id="HFO5817276.1"
                     /translation="MWYLLWFVGILLMCSLSTLVLVWLDPRLKS"
     gene            46444..47742
                     /gene="appA"
                     /locus_tag="ACHKBL_000352"
     CDS             46444..47742
                     /gene="appA"
                     /locus_tag="ACHKBL_000352"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acid phosphatase/4-phytase"
                     /protein_id="HFO5817277.1"
                     /translation="MKAILIPFLSLLIPLTPQSAFAQSEPELKLESVVIVSRHGVRAP
                     TKATQLMQDVTPDAWPTWPVKLGWLTPRGGELIAYLGHYQRQCLVADGLLAKKGCPQP
                     GQVAIIADVDERTRKTGEAFAAGLAPDCAITVHTQADTSSPDPLFNPLKTGVCQLDNA
                     NVTDAILSRAGGSIADFTGHRQTAFRELERVLNFSQSNLCLNREKQDESCSLTQALPS
                     ELKVSADNVSLTGAVSLASMLTEIFLLQQAQGMPEPGWGRITDSHQWNTLLSLHNAQF
                     YLLQRTPEVARSRATPLLDLIMAALTPHPPQKQAYGVTLPTSVLFIAGHDTNLANLGG
                     ALELNWTLPGQPDNTPPGGELVFERWRRLSDNSQWIQVSLVFQTLQQMRDKTPLSLNT
                     PPGEVKLTLAGCEERNAQGMCSLAGFTQIVNEARIPACSL"
     gene            complement(47862..50042)
                     /gene="etk"
                     /locus_tag="ACHKBL_000353"
     CDS             complement(47862..50042)
                     /gene="etk"
                     /locus_tag="ACHKBL_000353"
                     /EC_number="2.7.10.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
                     /protein_id="HFO5817278.1"
                     /translation="MTTKNMNTPPGSTQENEIDLLRLVGELWDHRKFIVSVTALFTLI
                     AVAYSLLSTPIYQADTLVQVEQKQGNAILSGLSDMIPNSSPESAPEIQLLQSRMILGK
                     TIAELNLRDMVEQKYFPIVGRGWARLTKEKPGELAISWMHIPQLNGQDQQLTLTVGEN
                     GHYTLEGEGFTVNGMVGQRLEKDGVALTIADIKAKPGTQFVLSQRTELEAINALQETF
                     TVSERSKESGMLELTMTGDDPQLITRILNSIANNYLQQNIARQAAQDSQSLEFLQRQL
                     PEVRSELDQAEEKLNVYRQQRDSVDLNLEAKAVLEQIVNVDNQLNELTFREAEISQLY
                     KKDHPTYRALLEKRQTLEQERKRLNKRVSAMPSTQQEVLRLSRDVEAGRAVYLQLLNR
                     QQELSISKSSAIGNVRIIDPAVTQPQPVKPKKALNVVLGFILGLFISVGAVLARAMLR
                     RGVEAPEQLEEHGISVYATIPMSEWLDKRTRLRKKNLFSNQQRHRTKNIPFLAVDNPA
                     DSAVEAVRALRTSLHFAMMETENNILMITGATPDSGKTFVSSTLAAVIAQSDQKVLFI
                     DADLRRGYSHNLFTVSNEHGLSEYLAGKDELNKVIQHFGKGGFDVITRGQVPPNPSEL
                     LMRDRMRQLLEWANDHYDLVIVDTPPMLAVSDAAVVGRSVGTSLLVARFGLNTAKEVS
                     LSMQRLEQAGVNIKGAILNGVIKRASTAYSYGYNYYGYSYSEKE"
     gene            complement(50062..50508)
                     /gene="etp"
                     /locus_tag="ACHKBL_000354"
                     /gene_synonym="yccY"
     CDS             complement(50062..50508)
                     /gene="etp"
                     /locus_tag="ACHKBL_000354"
                     /gene_synonym="yccY"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine-phosphatase Etp"
                     /protein_id="HFO5817279.1"
                     /translation="MAQLKFNSILVVCTGNICRSPIGERLLRKRLPGVKVKSAGVHGL
                     VKHPADATAADVAANHGVSLEGHAGRKLTAEMARNYDLILAMESEHIAQVTAIAPEVR
                     GKTMLFGQWLEQKEIPDPYRKSQDAFEHVYGMLERASQEWAKRLSR"
     gene            complement(50496..51635)
                     /gene="gfcE"
                     /locus_tag="ACHKBL_000355"
     CDS             complement(50496..51635)
                     /gene="gfcE"
                     /locus_tag="ACHKBL_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HFO5817280.1"
                     /translation="MKKNIFKFSVLTLAVLSLTACTLVPGQNLSTSNKDVIELPDNQY
                     DLDKMVNIYPVTPGLIDQLRAKPIMSQANPELEQQIANYEYRIGIGDVLMVTVWDHPE
                     LTTPAGQYRSASDTGNWVNADGAIFYPYIGRLKVAGKTLTQVRNEITARLDSVIESPQ
                     VDVSVAAFRSQKAYVTGEVSKSGQQPITNIPLTIMDAINAAGGLTADADWRNVVLTQN
                     GVKTKVNLYALMQRGDLRQNKLLHPGDILFIPRNDDLKVFVMGEVGKQSTLKMDRSGM
                     TLAEALGNAEGMNQDVADATGIFVIRATQNKQNGKIANIYQLNAKDASAMILGTEFQL
                     EPYDIVYVTTAPLARWNRVISLLVPTISGVHDLTETSRWIQTWPN"
     gene            complement(51681..53777)
                     /gene="gfcD"
                     /locus_tag="ACHKBL_000356"
     CDS             complement(51681..53777)
                     /gene="gfcD"
                     /locus_tag="ACHKBL_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HFO5817281.1"
                     /translation="MKKNSYLLSCLAIAVSSACHAEVLTYPDPLGSSQSDFGGTGLLQ
                     MPNARIAPEGEFSVNYRDNDQYRFYSTSVALFPWLEGTIRYTDVRTRKYSQWEDFSGD
                     QSYKDKSFDFKLRLWEEGYWLPQVAFGKRDIAGTGLFDGEYLVASKQAGPFDFTLGMA
                     WGYAGNAGNITNPFCRVSDKYCHRAESHDAGDISFSDIFRGPASIFGGIEYQTPWNPL
                     RLKLEYDGNNYQNDFAGKLPQASHFNVGAVYRAASWADLNLSYERGNTLMFGFTLRTN
                     FNDLRPALRDTPKPAYQPAPESEGLQYTTVANQLTALKYNAGFDAPEIQLRDKTLYMS
                     GQQYKYRDSREAVDRANRILVNNLPQGVEKISVTQKREHMAMVTTETDVASLRKQLAG
                     TAPGQSEPLQQQRVEAEDLSAFGRGYRIREDRFSYSFNPTLSQSLGGPEDFYMFQLGL
                     MSSARYWFTDHLLLDGGIFTNIYNNYDKFKSSLLPADSTLPRVRTHIRDYVRNDVYLN
                     NLQANYFADLGNGFYGQVYGGYLETMYAGVGSELLYRPLDASWALGVDVNYVKQRDWD
                     NMMRFTDYSTPTGFVTAYWNPPTLNGVLMKLSVGQYLAKDKGATIDVAKRFDSGVAVG
                     VWAAISNVSKDDYGEGGFSKGFYISIPFDLMTIGPNRNRAVVSWTPLTRDGGQMLSRK
                     YQLYPMTAEREVPVGQ"
     gene            complement(53777..54523)
                     /gene="gfcC"
                     /locus_tag="ACHKBL_000357"
     CDS             complement(53777..54523)
                     /gene="gfcC"
                     /locus_tag="ACHKBL_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC D2 domain-containing
                     protein"
                     /protein_id="HFO5817282.1"
                     /translation="MNKLQSYFIASVLYVMTPHAFAQGTVTIYLPGEQQTLSVGPVEN
                     VVQLVTQPQLRDRLWWPGALLTDSAAKAKALKDYQHVMAQLASWEAEADDDVVATIKS
                     VRQQLLNLNITGRLPVKLDPDFVRVDENSNPPLVGDYTLYTVQRPVTITLLGAVSGAG
                     QLPWLAGRSVTDYLQDHTRLAGADKNNVMVITPEGEAVVAPVALWNKRHVEPPPGSQL
                     WLGFSAHVLPEKYADLNDQIVSVLTQRVPD"
     gene            complement(54520..55164)
                     /gene="gfcB"
                     /locus_tag="ACHKBL_000358"
     CDS             complement(54520..55164)
                     /gene="gfcB"
                     /locus_tag="ACHKBL_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein GfcB"
                     /protein_id="HFO5817283.1"
                     /translation="MRPLILSIFALFLAGCTHSQQSMVDTFRASLFDNQDITVADQQI
                     QALPYSTMYLRLNEGQRIFVVLGYIEQEQSKWLSQDNAMLVTHNGRLLKTVKLNNNLL
                     EVTNSGQDPLRNALAIKDGSRWTRDILWSEDNHFRSATLSSTFSFAGLETLNIAGRDV
                     LCNVWQEEVTSTLPEKQWQNTFWVDSATGQVRQSRQMLGAGVIPVEMTFLKPAP"
     gene            complement(55271..55576)
                     /locus_tag="ACHKBL_000359"
     CDS             complement(55271..55576)
                     /locus_tag="ACHKBL_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5817284.1"
                     /translation="MKHKLSAILMAFMLTTPAAFAAPEAANGTEATTGTTGTTTTTTG
                     ATTTAATTGGVAAGAVGTATVVGVATAVGVATLAVVAANDSGDGGSHNTSTTTSTTR"
BASE COUNT        13696 a        14116 c        14670 g        13314 t
ORIGIN      
        1 cctggcacaa aatcctcttt cagacatcca gaatgccaaa aaatagtatg aaaattgtgc
       61 tatcagctaa gaaaaaacta atccgcaata agaatgcggg ttattttttg tgcgtaaatg
      121 ttgcaatgta atattccgta acaggatgat cgttatggcg gggttgcaaa tagattgctt
      181 gacttaacaa acagataact tgacagaaag gataaaaaaa gagccagcgg gtgctggctc
      241 ttgaagacgc taaattagaa gctggcgtta cgcggagtac gtgggaacgg aatcacatca
      301 cgcacgtttt gcacaccagt tacgtaagca atcaggcgtt caaaaccaag accgaaacct
      361 gaatgcggaa cagtaccgta gcgacgcaga tcgcgatacc accagtaatc ttctttattc
      421 aggcccattt ccagcatacg ctcgtccagc acgtccagac gttcttcacg ctgggagcca
      481 ccaatgatct caccgatgcc cggagccaga acgtccatag ccgcaacggt tttaccgtct
      541 tcgttaaggc gcatatagaa cgctttaata tctttcggat agtttttaac cactaccggt
      601 gctttaaagt gttcttccgc cagataacgc tcatgctcag aagagagatc gacgccccag
      661 taaaccgggt tttcaaactt cctgccgcag ttttcgagaa tggtcactgc gtcggtgtaa
      721 tccacctgcg cgaaatcggc ctcaatgaag cgttccagac gtgaaacggc atctttatct
      781 acgcgttcag cgaagaattt catgtcgtca gcgcgttctt cgagaaccgc tttgaagaca
      841 tatttcagca tggcttcagc cagacccgcg atatcgttca ggttagcaaa cgccacttcc
      901 ggctccagca tccagaattc cgccaggtga cggctggtgt tggagttttc agcacggaaa
      961 gtcgggccga aggtataaat tttggacaat gcgcaagcgt aggtttcgcc gttcaactgg
     1021 ccagatacgg tcaggaaaga ctctttacca aagaagtctt tgtcgaaatc cactttgccc
     1081 tgatcgttac gcggcagatt ttccagatcc agcgtagaaa cacggaacat ttcgcctgca
     1141 ccttcggtat cagatgcggt aatcagtggc gttgaaaccc agaaaaatcc ctgctcgtta
     1201 aagaagcgat gcagcgcctg cgccagcgta tggcgaacgc gcgcgacggc accaatcagg
     1261 tttgtgcgcg gacgcaggtg agcgacttca cgcagatact caatgctgtg gcgttttgcc
     1321 gccatcgggt aagtgtctgg atcttcaacc caaccagcaa cttcaacctt gctggcctga
     1381 atttcaaatt gttgcccctg gcccggcgac gccacgactt tacccgtcac aatgaccgag
     1441 cagccggtgg tcagacgcaa gacgtcttca ttgtaattgg gcagagaatt attgatgaca
     1501 gcctgtacag gatcaaagca ggaaccgtca taaacggcga ggaaggagat gccagctttt
     1561 gaatctcggc gggtacgtac ccatccgcgc acggtgactt cgctgtcaac ggctacgcgg
     1621 ccctggagta cgtcggctac aggcacaacg ctcataatat tctctctgtt aatagtcgga
     1681 aaaaataaac acttgtccac ccgtaaatgg ggggatacct atgttacctg gcatctgcaa
     1741 tcagacaagc agaattcgca aatgcagcga aagatttcgg aattaaaggt aaaaaaaggg
     1801 agccgattag ctccctgatg atattaactg gcttttttaa tatgcggaag gtcgaacgct
     1861 ttgcgtagcg cccgaacaaa cgctttatcg tggcagatag ttttgcctgg gctgtcagaa
     1921 agtttcgcca ctggtttacc gttacactct accaacttaa tgacaatatt caggggtttt
     1981 acctggggga tatcgcaggt caggcgagtc ccaataccaa aacttaattg cacgcgggaa
     2041 gagaagtggc ggtatagctc aaccgctttg cgtaaatcca gattgtcaga gaaaaccagc
     2101 gttttactct gtggatcaat tcccagcttt tcataatgtg caatggcttt ttcaccccat
     2161 tcaaccgggt caccagagtc atggcgcagg ccctgatagc gactggcgaa ctcgacaccg
     2221 aaatctcgca ggaaagcatc catagtgatg cagtcggtta acgcaatgcc aagttggtcg
     2281 ggatactctt ccagccaggc agcaagtgca gctcgctggc tgttggctag atccgggctg
     2341 atttgctgat gcgcctggaa ccattcgtgt gcctgtgttc ccatcggcgt gagggaaagc
     2401 cgacgcgcca gatcgtagtt gctggtgccc acgaaccagg attcctgttg cagacgtttc
     2461 acgatggttt cttgtacttc acgagaaaaa cgtcggcggg tgccaaaatc catcagatgg
     2521 aagcgcgaca tatcaagacc ggcggttaat gccgagaagt cggctaattt gctttccagc
     2581 gtgtcgaggg cttgcgcaac gtcggcctgc ggtgagcgat agcgatgtac catttcactg
     2641 ataaccgcca gcaaaggaac ttcccagagg atgacttcac gccacgggcc gcttaaacga
     2701 atatccagct tgccattatc gttggacacg gtgacttgtt ccgggttaaa gcggaactcg
     2761 cgtagccagt taagatagtc ggccttaaag aaaggcaggg cagagagcca ctgatattca
     2821 tcatcctgca ggcgcaggta ctgcatcgcc tgaacctgtt cacgaatagc atcggcataa
     2881 atacccagca gatcgtcacc tcggcaacga aactccgccg cgacatgcac atcgtaatag
     2941 tgatgaaaca cggcttgctg catatgcaac ttataagcat ctgtatccag caacgagtgc
     3001 agaacaggag aagcgaattg tgtcataggt gcgcagtagc gtcctcgtac aggagcgttt
     3061 agtacaataa acatcttcag gaactgctgg agtatacctt gtttcgcaat ttattgaacc
     3121 ccgatcacac catatgccac ctttctggtc gatggcatta cgcggtgtat gttataaaaa
     3181 tgtagcaata aaggcgtttg tacctgaaaa gatgaagatt ctgcatagcg cgatttacgc
     3241 aacaggaata gactgaacac cagactctat aaaagatgct aaaggttatt tatgactcaa
     3301 cagccacaag ccaaataccg tcacgattat cgtgcgccgg attaccagat tactgatatt
     3361 gacttgacct ttgacctgga cgcgcaaaag acggtcgtta ccgcggtcag ccaggctgtc
     3421 cgtcatggtg catcagatgt tccgcttcgt ctcaacggcg aagacctcaa actggtttct
     3481 gtttatatta atgatgagcc gtggaccgcc tggaaagaag aagagggcgc actggtcatc
     3541 agtaatttgc cggagcgttt tacgcttaag atcgttaatg aaattagccc ggcggcgaat
     3601 actgcgctgg aagggcttta tcagtcaggc gatgcgcttt gcacccagtg tgaagccgaa
     3661 ggtttccgcc atattacgta ttatctcgac cgcccggatg tgctggcgcg ttttaccacc
     3721 aaaattattg ccgataaaac caaatatccc ttcctgcttt ccaacggtaa ccgcgttgcg
     3781 caaggtgaac tggaaaatgg acgtcattgg gtacggtggc aggacccgtt cccgaaaccg
     3841 tgctacctgt ttgcgctggt ggcaggcgac tttgatgtgc tgcgcgacac tttcaccacg
     3901 cgttctggtc gcgaagtagc gctggagctg tatgtcgatc gtggcaacct tgatcgtgcg
     3961 ccgtgggcaa tgacctcgct gaaaaactcc atgaaatggg atgaagaacg cttcggcctg
     4021 gagtatgacc tcgacatcta tatgatcgtc gcggtggatt tcttcaatat gggcgcaatg
     4081 gagaataagg ggctgaatat ctttaactcc aaatatgtgc tggcccgcac cgacaccgcc
     4141 accgacaaag attacctcga tattgaacgc gttatcggcc atgaatattt ccataactgg
     4201 accggcaacc gtgtcacctg tcgcgactgg ttccagctca gtctgaaaga aggtttaacc
     4261 gtcttccgcg atcaggaatt cagctctgac cttggttccc gcgcggtaaa ccgtatcaat
     4321 aatgtgcgca ccatgcgcgg attgcagttt gcagaagacg ccagcccgat ggcgcaccca
     4381 attcgcccgg atatggtcat tgaaatgaac aacttctaca ccctgaccgt ttacgagaag
     4441 ggcgcggaag tgattcgtat gatccacact ctgcttggcg aagaaaactt ccagaaaggg
     4501 atgcagctct atttcgagcg tcatgatggt agcgcagcga cctgtgacga ctttgtgcag
     4561 gcgatggaag atgcgtcgaa tgtcgatctt tcccatttcc gccgttggta cagccaatcc
     4621 ggcacgccgg ttgtgactgt caaagacgac tacaatccgg aaactgagca gtacaccctg
     4681 accatcagcc agcgcacgcc agctacgccg gatcaggcag aaaaacagcc gctgcatatt
     4741 ccgtttgcca tcgaactgta tgataacgaa ggcaaagtga tcccgttgca gaaaggcggt
     4801 catccggtga attcggtgct gaacgtcacc caggctgaac agacctttgt ttttgataat
     4861 gtctacttcc agccggtgcc tgcgctgctg tgcgaattct ctgcgccagt gaaactggaa
     4921 tataagtgga gcgatcagca actgaccttc ctgatgcgtc atgcgcgtaa tgatttctcc
     4981 cgttgggatg cggcgcaaag cctgctggca acctacatca agctaaacgt cgcccgtcat
     5041 cagcaagggc agctgctgtc tctgccggtg catgtggctg acgctttccg cgcggtgctg
     5101 ctcgatgaga agattgatcc agcgctggcg gcagaaatcc tgacgctgcc ttctgtcaat
     5161 gaaatggctg aactgttcga tatcatcgac ccgattgcta ttgccgaagt acgcgaagca
     5221 ctcactcgta ctctggcgac tgaactggcg gatgagctac tggctattta caacgcgaat
     5281 taccagagcg agtaccgtgt tgagcatgaa gatattgcaa aacgcactct gcgtaatgcc
     5341 tgcctgcgct tccttgcttt tggtgaaacg catctggctg acgtgctggt gagcaagcaa
     5401 taccatgaag caaacaatat gaccgatgcg ctggcagcgc tttctgcggc ggttgctgca
     5461 cagctgcctt gccgtgacgc gctgatgcag gagtacgacg acaagtggca tcaggatggt
     5521 ctggtgatgg ataaatggtt tatcctgcaa gccaccagcc cggcggcgaa tgtgctggag
     5581 acggtgcgtg gcctgttgca gcatcgctca tttaccatga gcaacccgaa ccgtattcgt
     5641 tcgttgattg gtgcgtttgc gggcagcaac ccggcagcgt tccatgccga agatggcagc
     5701 ggttaccagt tcctggtgga aatgcttacc gacctcaaca gccgtaaccc gcaggtggct
     5761 tcacgcctga ttgagccgct gattcgcctg aaacgttacg atgccaaacg tcaggagaaa
     5821 atgcgcgcgg cgctggagca gttgaaaggg ctggaaaatc tctctggcga tctgtacgag
     5881 aagataacta aagcactggc ttgatacttt tgagccaaaa tgccggatgc gacgcttgac
     5941 gcgtcttatc aggcctacaa gtccgttgaa aatgctggtt ttgtaggctg gataaggcgt
     6001 tcacgccgca tccggcagcc gtgcccaaat gcctgatgcg acgcttgacg cgtcttatca
     6061 ggcctacaat ccgttgaaac tgctggtttt gtgtgccaca tccggcatat taaccatgac
     6121 gccgaatcgg ttgttcagag tgcccacgct gcatcacccg ttgcaaaact tccgcttcca
     6181 gctccgctaa tcgcaccgac cctaaccggc gcggacgggg gatatccacc gtcaaatcca
     6241 gaccaatttt tccctcttca attaacaata cccggtcagc cattgctacc gcttcgctca
     6301 catcatgcgt caccaacagt acggtaaagc cgtgctcctg ccaaagtgac acaatcaaat
     6361 cctgcatctc gagccgagtc agggcgtcca gcgccccgag cggttcatca agcaataata
     6421 aacccggtcg atgaattaac gctcttgcca gcgccacacg ctgtttctgc ccgccagaaa
     6481 gtgcggcagg ccattcccct gcgcggttct ccagtcctac tgcagccaac gcccgacgcg
     6541 cggcatcgcg ccactggcct ttaaggccta acccaacgtt atcaatcacc gatttccacg
     6601 gtagcagacg cgcatcctga aacatcattc gcgtgtcttc ctgaatctca gccagcggtg
     6661 tggtgcccgc taacacgtcg cctgcggttg gcgtttccag acctgccagc aggcgcagca
     6721 gggtactttt gccaccaccg ctgcggccca ccaccgccac aaactgacct gccggaatat
     6781 gtaaatccag ttggttcagg acgatatttt ccgcgtattg tttgcttact gcattgagca
     6841 acaatggtgt gccctggttc agacgagcag tattcatacc gtggcctcct tcaaatgata
     6901 agccgggttc cagcgcaacc agagacgctc taacagctgc gcgctgacgt cagcaagttt
     6961 gccgagcagg gcgtaaagaa taatagcgac taccaccacg tccgtttgca gaaactcccg
     7021 cgcattcatc gccagataac caatgccgga attggcagaa atagtttcgg caacgatcag
     7081 cgtcagccac atcaggccca aagcaaaacg cacgccgacc ataattgagg gcagggcgcc
     7141 aggcaggatc acatggataa acagcggtat gccggataat ccatagctac gcgccatttc
     7201 caccagaccg cgatcgatat tacggatgcc gtgccaggtg ttgatataaa tggggaatag
     7261 cgtgccgagc gccaccagaa agatttttgc ggattcatca atgccaaacc acaaaatcac
     7321 cagcgggatc agcgccagat gcggcacgtt acgcaacatc tgaatggagg tatccagcag
     7381 tcgttctccc cagcgagata gcccgctaat cagtcccaaa atcaacccca gcgatccgcc
     7441 aattgaaaag ccaatgagcg cccgccagga gctgatcgcc agatgctgcc acaattcgcc
     7501 gctggcggag agcgtccaga acgccatcac cactccttcc ggtgagggca aaatacgcgt
     7561 tgacagccag ccaactgagg aggccagttg ccacaccgcc acgatgccca ccggtaaaaa
     7621 ccagggggca acgcgcaata accacttttt cactggcgtt gccattatcg tattccttag
     7681 ctttgcgcga cgttacgggg gataaaatca tttgccaccg cttcgccttg cggattcagc
     7741 ggctgcggct ggggaatttc cgggatggcg acatccagat gcgggaacag caactcgcca
     7801 acccgatacg cttcttccag atgcgggtag cccgaaagca caaagctgtc gatgccaagc
     7861 gcggcgtatt cgttgattcg cgcagcgacc gtcggaccat cgcccaccag tgccgtcccg
     7921 gcaccgccgc gcactaagcc aacgcccgcc cataaattgg ggctgatctc cagattgtcg
     7981 cgcttgccgt tatgtagcgc cgccattcgc tgttgtccta cggaatccgt ccgggcgaat
     8041 gcggcttgtg ctttggcgat agtctcatca tcaagatgcg agattaaccg ctcggcggcc
     8101 tgccacgctt cgtcgttggt ttcacgaaca atcacatgca gacgaatacc gaaacgaatt
     8161 ttgcgtccat gcgcggcagc tttcgcccgc acttgttcga ttttctcttt aaccagttcc
     8221 ggcggttcgc cccaggtgag gtagagatca acttgttctg ccgccagctc ctgggcgaca
     8281 tctgacgatc cgccaaagta aagcggagga tacggctgtt gaatcggcgg gaagagcagt
     8341 ttggctccgc gcacatgaat atgtttaccg ttgaaattga cggtttcccc cagcaataag
     8401 cgccgccaga cctgggtaaa ttccgccgag gcttcgtagc gctcgctatg atcaaggaac
     8461 acgccgtcgc ctgccagctc ttgtggatcg ctgcctgtga ccagattaaa caacgcacga
     8521 ccattcgaga gacggtcaag cgtggcggcc tggcgggcgg caacggtagg tgaggttacg
     8581 ctgggacgca gggcgacaag aaacttcagc cgttgcgtca ccgggatcat cgacgcggca
     8641 accagccacg catcttcaca cgatcgtcct gttgggatca gcacaccggt ataacccaga
     8701 cgatccgccg cttgcgcaat ttgttgcaga taaccgtgat caaccggacg tgaaccttct
     8761 tccgttccca gatagtgccc gtcaccatgg gtcggtaaaa accagaacat attcagactc
     8821 ataattgttt tccttccagt tgagtgggct gccagatgcg ctggcgaata tcgacttttt
     8881 tcggcaccag acggttttca taaaacagat ctgccgtttg ctgctgtaag gcggcaactt
     8941 cagcgctcaa cggtttgatg gtggtgggag gacgatgatc cagatatgag gcaataaccg
     9001 gtgccggtaa gcccatcgtt tttgccagta aagcgatgct ttgttcgcgc tggctgcggg
     9061 ttaacgcatc ggcctcacta aaggttgtca gcacaccctg aataaaagcg ccgttttttt
     9121 ctgcatacgg acgagctgcc agataaaacg atccagtttg attgagatcg gtgccgtctt
     9181 tcagcacccg cacgccgccc tgtaataatg cagcggagta gtagggatcc cagatagccc
     9241 aggcgtcaac gttaccttgc tggaacgcgg cgcgggcatc ggcaggcgtc aggtaagtgg
     9301 gctgaatatc cgtgaattta agcccggcct ggcgaagggc tcgcagtaaa agattgtgcg
     9361 aactggaacc tttctgaaag gcaactttgt gacctttaag atcggctacg gttttgatcg
     9421 ggctgttttc tgccaccaga atcacttcgg ctttgggttt cggtggctcg acgcccacgt
     9481 ataccaaatc agccccggca gcctgggcaa agattggcgg aatatccccg gtactgccga
     9541 gatcgatact gccaacgttt aacgcctcca gcatctgtgg gccagcgggg aactccaccc
     9601 aggagatttt tgtttgcgga tagcgttttt ccagtaactg gtggcttttt gccagcacca
     9661 taccaatact gcctttttga tagcctatac gtaacgcctc tggcgaagac tccgcggcaa
     9721 cagcaagtgt agagacgcta agtaaacccg ccagcgccag tttaatgatc ttacgcatgg
     9781 gcattacctc gcagagacag aaggtcagga acctgaacat cgcggcggtg caacgcctgc
     9841 cagaaagttt ctagcgcggt atcaagacga gtttgcagat ttggcgtgaa ctggggtttg
     9901 tgttggtaat caattacttg cgagtcatcg gcaaacacgc cgtgcaggat ctcctgggct
     9961 ttcagcgtac ttaaaactgg cttaagggca taatcgaccg ccagcagatg ggccacggtg
    10021 ccgcctgtcg ccagcggcag caccactttg ccttgcaacg cacgttctgg cagcaggtcg
    10081 agcagggttt tcagcgcgcc ggaataggcg gctttataca caggcgtggc gacaatcagc
    10141 ccatcggcct gttgcagctg ttcggtgaag gtcttgagtg ccggactatc gaaacgagca
    10201 taaagcagat cttccgggac gaagttttgc aaattccagt gataaacctc tacatccagg
    10261 ccatttagtt tttcccgcgc atattccagc aaggagctgg agcgagaagg aaagcgagga
    10321 ctacccgcca gggtgatgac acgcatactc tctccttata accaattgtt cttgttttgt
    10381 taacattgat aacaattcgg tcagtctgac agagggcgtc cgcgagtcga aatgatttat
    10441 ctgaggaaga aaagccaaaa aaagcataaa aaaagcgcaa taagaaaacg tttgctattt
    10501 attgccgcag gtcaattccc ttttggtccg aactcgcaca taatacgccc ccggtttgca
    10561 caccgggaat ccaggagagt tcatgtacta ccccttcgtt cgtaaagccc ttttccagct
    10621 cgatccagag cgcgctcatg agtttacttt tcagcaatta cgccgtatta ccggaacgcc
    10681 gtttgaagca ctggtgcggc agaaagtgcc tgcgaaacct gttaactgca tgggcctgac
    10741 gtttaaaaat ccgcttggtc tggcagccgg tcttgataaa gacggggagt gcattgacgc
    10801 gttaggcgcg atgggatttg gatcgatcga gatcggtacc gtcacgccac gtccccagcc
    10861 aggtaatgac aagccgcgtc tctttcgtct ggtagatgcc gaaggtttga tcaaccgtat
    10921 gggctttaat aatcttggcg ttgataacct cgtagagaac gtaaaaaaag cccattatga
    10981 cggcgttctg ggtattaaca tcggcaaaaa taaagatacg ccggtggagc agggtaaaga
    11041 tgactatctg atttgtatgg aaaaaatcta tgcctatgcg ggatatatcg ccatcaatat
    11101 ttcatcgcca aataccccag gattacgcac actgcaatat ggcgaagcgc tggatgatct
    11161 cttaaccgcg attaaaaata agcaaaatga tttgcaagcg atgcaccata aatatgtgcc
    11221 gatcgcagtg aagatcgcgc cggatctttc tgaagaagaa ttgatccagg ttgccgatag
    11281 tttagttcgc cataatattg atggcgtgat tgcaaccaat accacgctcg atcgttctct
    11341 ggttcaggga atgaaaaatt gtgatcaaac cggtggctta agtggtcgtc cgcttcagtt
    11401 aaaaagcaca gaaattattc gccgcttgtc acaggaatta aacggtcgct taccgatcat
    11461 cggtgttggc ggcattgatt cggttatcgc tgcgcgtgaa aagattgctg cgggggcctc
    11521 actggtgcaa atttattctg gttttatttt taaaggtccg ccgctgatta aagaaatcgt
    11581 tacccatatc taattatttc ttcgaattcc tctttataag ataatcaggg ctttatttta
    11641 agccctggtt gttttatatt catcgctgtt gcttatttag acattttgta cttttattgt
    11701 tgaggttatt aagcgaagcg acaatggatt gtgttgctgc ggttttatag ggtaggggag
    11761 aggcagatgc gaattaaacc agacgataac tggcgttggt attacgatga agagcacgat
    11821 cgaatgatgc tcgatttagc caatggtatg ctatttcgct cacgttttgc gcgcaaaatg
    11881 ttgactccag atgccttttc tcccgcaggc ttttgcgttg acgacgccgc gctctatttc
    11941 tcatttgaag aaaaatgccg cgattttaat ttatctaaag aacaaaaagc cgaactggtg
    12001 cttaatgcgc tggtagcaat tcgttatctc aaaccgcaaa tgccgaaaag ctggcatttt
    12061 gtttcccatg gtgaaatgtg ggtgcccatg ccgggcgatg cggcctgcgt ttggttgagc
    12121 gacactcacg agcaagtcaa tttgctggtc gttgaatctg gcgaaaatgc cgcactatgc
    12181 ctgctggcac aaccctgcgt tgttattgcg ggtcgcgcga tgcagctggg cgacgccatc
    12241 aaaatcatga acgacaggct gaaaccgcag gttaatgttg acagcttcaa cctcgaacag
    12301 gcagtctaac gcgtcagctt aagcgcagtc ttcggtacac agctacagca aagaatggtg
    12361 ccatcatcgc ccattgctga ttttttcagc ggcgtgactt cgccttctaa aagctgaaca
    12421 cggcaacttc cacaaatgcc cgcgcggcaa gaataaggga tacgaattcc ctgattttct
    12481 aattgctcca gcaacacctg ctggttattt ccacgaaatg cctgtccctg ccagtcaata
    12541 tctacatttg cgtccggttg ttgcgtgatg ttgacggtat catcagcggc ggctgcgccg
    12601 taaattttag ccggagccgt tgccagaact tccacctcat cgccaacgcg aattacgccg
    12661 ctattacggg caattaaatt ctgaccaaaa tcgacatcgc cgttatcctg ggcagtgcgg
    12721 aaagattgca atgtttttaa tggttcgcct gccggatgtt tttgcccttt ttctggactg
    12781 acggtggtga aaatacagcg gctacaaggt ttaaccacat caaacaccac atcaccaatg
    12841 cgaatcactt tccagctatc ttcttcccag gctgacgcgc cggaaaccac cagattgggg
    12901 cggaattgct ccatttttac actggccgga caacgttgtt ggagatcacg taacgaggct
    12961 tcgttagcaa gaaggtaagg atagccatca gcaaatgaca ggggtacagt gttgtggcgt
    13021 ttcacgcgcc gcgtcatttg tggccccacc cagcgcaact gcacttcgcg ggagaaaaat
    13081 ccacttagcc atttgttgat cgcgtctggt gcaattcgcg cggtaaaatg tgtgccccaa
    13141 acttcggttg gtgcgtcttg tgtggcgaaa tcagcaaaac gaacatatgc gctactgcca
    13201 tctggtgcgg tgagatgcaa gccatcatgc acaggtgaag gagtaaaccg taccatctgg
    13261 ggaaactggc gagcggtaat aaacgtaccg tcaggttccg tgatcataaa gatgcgatcg
    13321 aaggccagac cactgacatc tgccagggca tgtgtaagac caatgccgcg catcgattta
    13381 acaggatgaa taaaaagccg ggttaatgtc gccacggtgc ggtcctcaaa tgaaaataag
    13441 ccctcaactt tatgacatga cgcgcttatt agctataatg cgcaacaatt ttcttagcgc
    13501 ctgtcccatt aggctatttt atttgtcagt ttggcccagg gaagtgctcg aaatcctcac
    13561 gtacttgtgt acgctccggt ttctccgcgc tgtccatgtc cagactgact gcaacaatta
    13621 cgcctactgc ggtaggctct tagagtaaaa gtgacgatat gaattctctg tttgccagta
    13681 cggcccgtgg gctggaagag ctgttaaaaa ctgaactgga aaacctgggg gccgttgaat
    13741 gccaggtggt tcagggtggg gtccatttca agggcgacac acggcttgtt taccagagcc
    13801 tgatgtggag ccgcctggcc tcgcgtatta tgttgccgct gggcgagtgt aaggtttaca
    13861 gcgatttaga cctctatctc ggtgttcagg cgatcaactg gacagagatg tttaatcctg
    13921 gcgcgacctt cgctgtccac ttcagtggtt tgaatgacac catccgcaac agtcagtacg
    13981 gcgcgatgaa agtgaaagac gcgatcgtcg atgctttcac gcggaaaaat ctgccgcgtc
    14041 caaatgttga tcgcgatgcg ccggatatcc gcgttaacgt ctggctgcat aaagaaaccg
    14101 ccagtattgc ccttgatctc agtggtgatg gtttacatct gcgtggctat cgcgatcgtg
    14161 ctggtattgc gccgatcaaa gaaaccctgg cagccgcgat tgttatgcga tccggctggc
    14221 agccaggaac accgctgctc gatccgatgt gtggttccgg tacgttgttg attgaagcgg
    14281 cgatgctggc gaccgatcgc gcaccaggct tgcaccgtgg acgttggggc tttagcggct
    14341 gggcacagca tgatgaagcc atctggcagg aagtgaaagc agaagcgcaa acccgcgccc
    14401 gtaaaggcct ggctgagtat agctctcatt tttacggttc ggacagcgac gcacgggtga
    14461 ttcaacgtgc gcgcactaac gcccgtcttg cggggattgg tgaactgatc acctttgaag
    14521 tgaaagatgt cgcgcaactg accaatccgc tgccgaaagg gccgtacggt acagtgttga
    14581 gcaacccgcc atacggtgaa cgtctggaca gcgaaccggc gctgattgcg ctgcatagtc
    14641 tgctgggccg aatcatgaaa aaccagttcg gcggctggaa tctctcttta ttcagtgcct
    14701 cgccggatct gttaagttgc ctgcagttgc gtgcagacaa acagtacaaa gcgaaaaacg
    14761 gtccattaga ctgcgtgcag aaaaattacc acgttgccga aagcacacca gacagcaaac
    14821 cggtaatggc agcggaagac tacgccaacc gtctgcgtaa gaacctcaaa aaattcgaga
    14881 agtgggcgcg tcaggaaggc attgaatgct accgcctgta cgacgccgat ctaccggaat
    14941 acaacgttgc agttgaccgc tacgccgatt gggtggtggt gcaggagtat gcgccgccaa
    15001 aaactattga tgcgcacaaa gcgcgtcagc gtctgttcga tattatcgct gcaaccattt
    15061 cggtattggg gattgcgcca aacaaactgg tgctgaaaac ccgtgaacgc cagaagggca
    15121 aaaatcagta ccagaaactg ggcgagaagg gcgagtttct cgaagttacc gaatataacg
    15181 ctcacttgtg ggtgaacctg acggattacc tcgataccgg tctgttcctc gatcaccgta
    15241 tcgcccgtcg tatgctcggt cagatgagca aaggcaaaga tttcctcaac ctgttctctt
    15301 ataccggcag cgccaccgtg cacgcgggat taggcggtgc acgcagcact accaccgtgg
    15361 atatgtcgcg tacttatctg gagtgggcag aacgcaacct gcgtctgaat ggcctaaccg
    15421 ggcgtgcgca tcgcctgatt caggccgatt gcctggcgtg gctgcgtgag gcaaatgaac
    15481 agttcgatct gatttttatc gatccgccaa ccttctctaa ctcaaaacga atggaagatg
    15541 cgtttgatgt tcagcgcgat catctggcgc tgatgaaaga tttgaaacgt ctgctgcgtg
    15601 ctggtgggac gatcatgttc tcgaacaaca aacgtggctt ccgtatggat cttgacggac
    15661 tggcaaaact gggactgaaa gcacaagaaa ttacgcaaaa aacgctctcc caggatttcg
    15721 cccgtaaccg ccagatccac aactgctggc tgattaccgc agcctgaaag gaatagtaat
    15781 gtcattaatc agtatgcatg gcgcatggct gtcgttcagc gacgcgccgc ttctcgataa
    15841 cgcagaactg catatcgaag ataacgaacg tgtttgtctg gtgggccgca acggcgcagg
    15901 caaatcgacg ttaatgaaaa tcctcaaccg tgaacaaggg ctggatgacg gtcgcattat
    15961 ttacgagcaa gatttgattg tagctcgtct gcaacaggat ccgccgcgta acgttgaggg
    16021 gagtgtttat gatttcgttg ccgaaggcat tgaagaacaa gcggaatacc tgaaacgcta
    16081 tcacgatatt tcgcgcctgg tgatgaacga cccgagcgag aaaaatctca acgaactggc
    16141 gaaggttcag gaacagttgg atcaccacaa cctgtggcag ctggaaaacc gcattaacga
    16201 agtgctggcg caactggggt tagatcctaa cgttgcgctg tcgtcgcttt ccggcggctg
    16261 gttgcgtaaa gcggcattag gacgcgcgct ggtgagcaat ccgcgcgtat tgctgctcga
    16321 cgaaccgact aaccacctgg atatcgaaac catcgactgg ctggaaggat tccttaaaac
    16381 cttcaatggg acgataattt tcatctccca cgaccgttcg tttatccgca atatggcgac
    16441 gcgcattgtt gatctcgatc gcggcaagct tgtgacctat cctgggaatt acgaccagta
    16501 cctgctggaa aaagaagaag ccctgcgcgt ggaagaacta caaaatgccg agttcgatcg
    16561 caaactggcg caggaagagg tgtggatccg ccaggggatc aaagcacgcc gtacccgtaa
    16621 tgaaggccgc gtacgcgccc tgaaagcaat gcgtcgtgag cgtggcgaac gtcgcgaagt
    16681 gatgggtacc gcaaagatgc aggtggaaga ggccagccgc tccggtaaga tcgttttcga
    16741 aatggaagac gtttgctacc aggttgacgg taagcaactg gtgaaagatt tttctgccca
    16801 ggttctacgt ggcgacaaaa ttgccctgat tggtccgaat ggatgcggca aaaccacgct
    16861 gctgaaactg atgctcggtc agcttcaggc ggacagcggg cgtattcacg ttggcaccaa
    16921 actggaagtg gcttatttcg atcagcaccg tgcggaactg gatcccgata aaacggtgat
    16981 ggataacctt gctgaaggta agcaagaggt gatggttaac ggcaagccac gtcatgtttt
    17041 gggctatttg caggacttcc tgttccatcc gaaacgggcg atgacgccgg tacgtgcgct
    17101 ttctggcggt gagcgtaacc gcttgctgtt ggcgcgtttg ttcctcaaac caagcaactt
    17161 attgattctt gacgaaccga ccaacgatct tgatgttgaa acgctggaac tgctggaaga
    17221 actgatcgac agctaccagg gcacggtatt gctggtgagc cacgatcgtc agtttgtcga
    17281 taacaccgtt acagaatgct ggatcttcga aggcggcggt aaaattggtc gttatgtcgg
    17341 cggttatcat gatgcccgtg gtcagcaaga acagtatgtg gcgctcaaac agcctgcggt
    17401 gaaaaaaatc gaagaagccg ccgcgccaaa agcagaaact gtaaaacgca gcagtagcaa
    17461 actaagctat aaattgcagc gcgaactgga gcagctaccg caattgctcg aagatctgga
    17521 ggcgaagctg gaagccctac agacgcaggt ggcggatgct tccttcttca gtcagccgca
    17581 tgagcagacg caaaaagtgc tcgccgatat ggctgctgca gagcaggagc tggagcaagc
    17641 ctttgaacgc tgggagtatc ttgaagcgtt aaaaaatggt ggctgatcgc aaaatagtcg
    17701 atttaaacag agacagctga caacggtaaa attgcctgat gaactgggtt tatcaggcat
    17761 gaaaaccaac actttgttag tcatctgtaa aatagcgcat cattaaggag taccaatgtg
    17821 cgaacatcat catgccgcga agcacatcct gtgctcgcag tgtgacatgc tggtggcgtt
    17881 accgcgcctt gagcatggtc agaaagcggc atgtccccgg tgtggcacaa cgttaaccgt
    17941 ggcgtgggat gcccctcggc agcgtcccac cgcctatgcg ttggctgcac tgttcatgct
    18001 gttgctgtcc aacttgtttc cttttgtgaa tatgaacgtt gcgggagtca ccagtgaaat
    18061 tacattactg gaaattcccg gcgtgctttt ttctgaggac tacgccagcc tcggcacctt
    18121 tttcctgttg tttgtgcaac tggttcccgc gttttgtctg ataaccattc tgttactggt
    18181 gaatcgcgca gaattaccgg tccgtttaaa agagcaactg gcacgggtgc tttttcaact
    18241 caaaacctgg ggaatggcgg agattttcct cgcgggtgtg ctggtcagtt tcgttaaact
    18301 gatggcttac ggcagcattg gcgtaggcag cagctttctc ccctggtgtt tattttgtgt
    18361 cctgcaactg cgtgcttttc agtgcgttga tcgtcgctgg ttatgggacg acatcgcccc
    18421 gatgccagaa ctgcgccagc cgctaaaacc tggcgtcacg gggatacgcc aggggttacg
    18481 ttcgtgctcc tgttgtacag caattcttcc agctgatgat ccggtgtgtc cgcgctgtgg
    18541 tactaaaggg tacgttcgtc gtagaaacag cctgcaatgg acactcgcgc tgcttgttac
    18601 gtccatcatg ctgtatcttc cggctaatat tttgcccatc atggtgacgg atttattagg
    18661 ctcgaagatg ccatcgacga ttctcgctgg ggtcatcctg ttatggagcg aaggatctta
    18721 tcccgtcgct gcggttatct ttctggccag tattatggtg ccaacgttaa agatgatcgc
    18781 catagcgtgg ttgtgttggg atgccaaagg gcatggcaag cgcgacagtg aaagaatgca
    18841 tttgatttat gaagttgttg agtttgtagg ccgctggtcg atgattgacg ttttcgttat
    18901 cgcggtgctc tcggcgctgg tgcgtatggg aggtttaatg agtatttatc cggcaatggg
    18961 tgcattaatg tttgctttag tcgtcataat gacaatgttt tctgctatga cgtttgaccc
    19021 gcgtttgtcg tgggatcgtc aacctgaatc agagcatgag gagtcctgac agtatggaat
    19081 ctaataatgg ggaagccaaa atccagaaag tgaagaactg gtctcccgtg tggatatttc
    19141 ctatcgtcac ggcgctcatt ggggcctggg ttctttttta tcattacagc catcaggggc
    19201 cggaagtgac cctgatcacc gcgaatgcgg aaggaataga aggtggtaaa accaccatta
    19261 aaagccgtag cgttgacgtc ggcgtggttg aaagcgccac actggctgat gatttgacgc
    19321 acgttgaaat caaagcgcgg ctgaattccg gtatggaaaa attgctgcat aaagataccg
    19381 tcttttgggt ggtgaaaccg cagattggtc gagaagggat tagcggcctg ggaacgctgc
    19441 tgtctggtgt ttatatcgaa ctgcagccag gcgcgaaagg cagcaaaatg gataaatacg
    19501 atttgctgga ctcaccaccg ttggccccgc ctgatgcgaa aggtatccgt gtgattctcg
    19561 atagcaaaaa agccgggcag ctctcgccag gagatcccgt gctgttccgt ggctatcggg
    19621 taggttcggt tgaaaccagc acctttgata cgcaaaaacg caatatcagc tatcaactgt
    19681 tcatcaatgc accttatgac cgactggtga ccagcaatgt tcgcttctgg aaagatagtg
    19741 gcattgcggt tgatttgacg tcagcgggaa tgcgtgtgga gatgggttca ttgacaacgc
    19801 tgcttagtgg cggtgtcagc tttgatgtgc cggaaggtct ggatttaggg cagccagtgg
    19861 caccgaaaac agctttcgtt ttgtatgatg atcagaagag cattcaggat tcgttgtaca
    19921 ccgatcacat tgattatctg atgttcttta aagattcggt acgcggtctg caaccgggag
    19981 ctccggtaga attccggggt attcgcctgg gtaccgtaag caaagtgcca ttctttgcgc
    20041 cgaatatgcg tcagacattt aacgatgatt accgtattcc ggtactgatt cgtatcgagc
    20101 cagagcggct gaaaatgcag cttggcgaaa atgcggatgt tgttgagcac cttggcgaat
    20161 tgttgaaacg tggtttacgc ggatcgctga aaaccggaaa cctggtcact ggcgcactgt
    20221 atgttgatct cgatttctat ccaaatacgc ctgcaataac cggtattcgt gaatttaatg
    20281 gttatcagat tatcccgacc gttagcggcg gtctggcgca aatccagcaa cgactgatgg
    20341 aagcgttgga taagatcaac aaactgccat tgaatccgat gattgaacag gcaaccagta
    20401 cgctttctga aagtcagcgc acaatgaaaa acctgcaaac gacgctggat agcatgaaca
    20461 agatcctcgc cagccagtcg atgcagcagc tgccgacgga tatgcagtca acgttgcgtg
    20521 aattgaatcg cagcatgcag ggcttccagc ctggctccgc agcctacaac aagatggtgg
    20581 cggatatgca gcgccttgat caggtgttgc gagaactgca accggtgctg aaaacgctca
    20641 atgagaagag taacgcgctg gtatttgaag cgaaggacaa aaaagatcca gagccgaaga
    20701 gggcgaaaca atgaaaaagt ggctagtgac gattgcggca ctgtggctgg ccggatgcag
    20761 ctccggcgaa attaataaaa actattacca gttacctgtg gtgcagagcg gtacacaaag
    20821 caccgccagc cagggtaatc gcctgttatg ggttgagcag gtcgctgttc ctgactatct
    20881 ggcggggaat ggtgtggttt atcaaaccag tgatgtgaag tatgtgattg ccaacaacaa
    20941 cctgtgggcc agcccgttgg atcaacagtt gcgcaacact ctggttgcca acctgagcac
    21001 gcaactgccc ggctgggtgg ttgcctccca gcctctggga agcgcccagg atacgctcaa
    21061 tgttaccgta acggagttta acgggcgata tgatggcaag gtcattgtca gtggtgagtg
    21121 gctgttgaac caccagggac aactgatcaa acgtccgttc cgtctggaag gggtgcaaac
    21181 tcaggatggt tacgatgaaa tggttaaagt gctggccagt gtctggagtc aggaagccgc
    21241 ttctattgcg caagagataa agcgtctacc gtaatcataa agatttgtaa atataaccgt
    21301 ctccggtatg ttgcctgagg cggttttttt gtttctaagg tgcggaaaaa gttgttcctc
    21361 ttcacatttt ttgtacaact gacatgcccg tgtagctcac aaatatgaca gtggcgtgaa
    21421 ttttgcgcat tgacggcagt tatgattcgc ggtattgctt aactgtgatt gcacatttag
    21481 taatcactgt tttcttttcc accagaaacc agtatgaggg aaacgaggca tgaagagaca
    21541 aaaacgagat cgcctggaac gggcacatca acgtggttat caggccggca tcgccggacg
    21601 ctcaaaagaa atgtgtccct atcagacgct gaatcaaagg tcacaatggc tgggaggctg
    21661 gcgagaagcc atggcggaca gggtagtaat ggcctgattc tgtctcttta aaaagaaacc
    21721 tccgcattgc ggaggtttcg ccttttgata ctctgtctga ttataatcag aaggcagacg
    21781 tatcctggaa cagaccgact ttcaggtcgc tggcggtata gatcagacga ccatcaacca
    21841 gcacttcgcc atctgccaga cccataatca gacgtcgatt aacaatgcgt ttaaagtgaa
    21901 tacggtaggt cacttttttc gctgtcggca gtacctgacc ggtgaatttc acttcgccaa
    21961 cgcccagcgc gcggccttta ccttcgccgc ccagccagcc gaggtagaac cctaccagct
    22021 gccacattgc gtccaggccc aggcatcccg gcataaccgg atcgccaatg aagtggcatc
    22081 cgaagaacca cagatccgga ttgatatcca gttctgcttc aacataccct ttgtcgaagt
    22141 taccacccgt ttcggtcatt ttgaccacac ggtccatcat cagcatgttc ggtgctggca
    22201 attgcgggcc tttagcgcca aacagttcac cgcgaccaga ggcaagaagg tcttcttttg
    22261 tataggattc gcgtttatct accatgttct ctgtaagcct tattttattg aagcacgcag
    22321 gatagctaac acgtgtacgc tgaacaagtc cgatcagttc ggaataaacc agttcagcca
    22381 acgcaatggc catggaaaac ggtgacgtcc ttcctgttgc gatgcttgcg cgatacgttc
    22441 ctggatggtt tgcatcagcg tcgtttggcc ttcgccatcc cacaccagat ttaataataa
    22501 cggcagtgcg tcagtcacat cgtctactgc ccagatggtg aatttgcctt cttctaccgc
    22561 tttcaccagt tcactgtgaa gacttaaatg gcgaatgtta gcagtgggga taatgacacc
    22621 ttgtttcccg gttaactcac gttgctggca aatagcaaag aagccttcga ttttctcatt
    22681 taaaccaccg accggctggg cacgaccgaa ctgatcgact gaacctgtga tagcgatact
    22741 ctgattcacc ggcacatcgg cgagggcgct tatcagggcg cagagttcag ccatcgaggc
    22801 actatcgcca tccacttcac tgtatgactg ctcaaatgtc agcgatgctg agaaggggat
    22861 ctgttgctca agctgtagtt ccgacatcag gaacgcctgc atgatcatca tccctttcgc
    22921 atggatattg ccgccaagct ccgctttgcg ttcgatgtcg gtgaactcac catcgccaat
    22981 atgcacaacg cagctaatgc gagaaggttc gccaaaagcg cgtggatgac ccggaaattc
    23041 aatgaccgaa agggcgttaa tttgcccgat gcgttcgcct tcggtttcaa tcaggatttg
    23101 ctcctgaaga atctcatcct gcatgcgttc agcgaggaaa ccttcacgcc attcacgctg
    23161 ctgcagcatt aagtttagct gctcgccgga gaaggtgtcg ccatcgcaca gggaggcgac
    23221 ctctttacac tggcggagga tccactgcgg gctaagcgga agtgtttctt gttcaccggt
    23281 gtagcgggct gcttcgcgga taagtaccgg ccaggcatcc gctcccggtg caggtaagtg
    23341 attatgtctt gcggtcaatg ttacccagcg acaccactgg cttactgact ccgcatcgac
    23401 aatctgcaga gtatcttcaa attcgctata aatagcctgc tctgaaagct ctggttccat
    23461 ctcctggaaa tcagccaatg attcgcgttc gcctaccaga atgactttca gcttcaatgg
    23521 catcgaaggc acagagacgg ggagagggcg cgactcatca aacgcaaccc agtcaaaacg
    23581 ctcgcggtta acgatatttt tcagccgcat ccacagcaga ggttgcgcca gcagtgtacg
    23641 caaagagatg atgagaatac cgccatttgc ctgatgcacc agacctggct gcagggtaat
    23701 gtcgccatta aactggcgca ggcagccaaa gagttgttcc gcttctaccc agtcggcagc
    23761 gacaacttgc gttaaagtcg caaaattatc atctgcactc actgcgttac gtaagcggat
    23821 ggtgtgcccg gaaacctcat agtgaccgcc caccagttgg cctgcatcgc tttgtagcgc
    23881 acgcgcggca tcggcaataa gattcagata ctcagactct tccggggcct tcgccagcat
    23941 aaaagaggag gatgctcgcg tatgcagcaa ctgctccagc gcaaattgca gtcgcggttg
    24001 agtatcactg aataaaggat cgttttcgtc aatcaaatgt ggctgagcaa atatttcctg
    24061 atagctatcg gtatcaggaa ccaggtcacg ccatgcaagt ttcgtaatgg tcaaagttga
    24121 tgttttttag tctgttgtca aagccgcgat tataccgtaa ccggcactac agcacacgta
    24181 gaaagcaccg acaatactcc tggcatgggc gttaaagctc acaggatgga gattcttttc
    24241 ttcactggcc caaaaagctg atattctgta aagagttaca cggtaacatt gagatcgcta
    24301 tgaaatatca acaacttgaa aatcttgaaa gcggttggaa atggaagtat ctggtgaaga
    24361 agcaccgcga aggggagtta atcacccgtt acatagaagc cagtgccgcc caggaagccg
    24421 tcgatgtgtt gctctcgctg gaaaatgaac ccgtgctggt aaatggctgg attgacaagc
    24481 atatgaatcc ggaactggtc aatcgcatga agcaaaccat tcgggccaga cggaagcgcc
    24541 attttaatgc ggaacatcag catacgcgca aaaaatcgat cgatctggaa tttattgtct
    24601 ggcaacgtct ggctggtctt gcgcagcgtc gcggtaaaac gctttctgaa acgattgttc
    24661 agctgattga agatgcggaa aacaaagaga aatacgcgaa taaaatgtct tctttgaagc
    24721 aggatctgca ggcattgctg ggtaaggaat aactgacgaa agtcagttca attttctaaa
    24781 gacgaaaaaa aaccccgcag cagcggggtt ttttaccaga cgagaactta agcctgcggc
    24841 tgagttacaa cgtctttgat gcctttaact tcgatctcta cgcgacgatc cggagccagg
    24901 caatcgatca gtgcagcacg ctgtttcacg ttgtcacagg tgttgccagt aaccgggttg
    24961 gattcgccca taccacgtgc ggagattttg tctgccggga tacctttaga gatcaggtaa
    25021 tcaacaacgg actgagcacg acgctcggac agagcctggt tataagcgtc agaaccgatg
    25081 cggtcagtgt aacccagaac aactacggaa ccgtctttcg gatccaggtt gctcagctgg
    25141 ctgtacagct gatccagagc agcctgacct tccggtttca gggttgcttt gttgaaggtg
    25201 aacagaacgt cagacttcag agtgaagtgc ttggtctgta cttccggtgc cggagctgga
    25261 gccggagcaa ctactggagc tgcttcgccc tgaccgaaac ggtaggaaac acccaggctc
    25321 agcatgccgt tgtccggacg agtgccgatg gtgtgtgcgt caccgatgtt gttggtccac
    25381 tggtattcca gacgggtagc gatttcagga gtgatcgcat actcaacacc gccagcgaag
    25441 accggagaaa cgccggtgtc gtggtcttta gtggatgcgc caccaggtac gttagacttg
    25501 gtgtctgcac gccataccat accacccaga cgagtgtaaa cgtccagatc gtcagtgatt
    25561 gggtaaccca gtttagcggt cagctgaacg ccctgagctt tgtatgcgcc gttgatgttg
    25621 tcgcctttgt acggcatacg acctaaccag tcgtaaccca tttcaaagcc aacatacggg
    25681 ttaacctggt aaccaccaaa agcacctgca cccagttggt tttcgtgggt cggaccattg
    25741 ttaggaataa aaccagtgtc atggtactgg gaccagccca gtttagcacc agtgtaccag
    25801 gtgttatctt tcggagcggc ctgcgctacg gtagcgaaac cagccagtgc cactgcaatc
    25861 gcgatagctg tctttttcat tttttgcgcc tcgttatcat ccaaaatacg ccatgaatat
    25921 ctccaacgag ataacacggt taaatccttc accggggatc tgctcaatat taactctacc
    25981 gatatcttcg gcttatgccg agcacccctg gcgatgtaaa gtctacaacg tagttgaaaa
    26041 cttacaagtg tgaactccgt caggcatatg aaaaaaaagt cttgtataag gtacgtttaa
    26101 tcttttttgt cagcgacaat ttacagaaga aaatcgcgga aaccgcttca gacaagcctc
    26161 cgcaaggaaa attagtcacg actgaaagca ttggctgggc gacaaaaaaa gttccaggat
    26221 taatcctaaa tttacttaat gatacaaatt agagtgaatt tttagcccgg aaagttgtct
    26281 cgttgcgtga gaggatgcgc ttaccggacg cataataaac cccatagcgt taccttcatt
    26341 tgccgcatca acaagttcag catgctcttc ttcagtcaaa tcatctgcca accaaccgat
    26401 caccacgctg taattgcccg tgcgtaaagc gcgaaccatt gactcaacgg tgtggcaagg
    26461 ggagagctgg ctaatctgca ttactttcgt taagggtagc ccagatgcct gaacccattc
    26521 gcgactcagt ttttgttgcg gtgttaacca gagttgccag cgcgattgct gaccgagttg
    26581 ctgtaacaat ggcaacagta gaagttgcgt catcatgggc tgatcttcgc gatagacaac
    26641 ttcactgata agcccggctg tagtgttttc cgtagagaca cgcgcaattt tacttgctgc
    26701 ggatgagaac gacgaagcac gatgtgcata gcctgaagtg tacataatca atccagccct
    26761 gtgggttact gtatgaatat acagtacatc cggtgacgac aaagatcaac cctattttcg
    26821 gaaagagcct cgcaaatttt gtcgttagtg acgggaaaac ataaattaat cttgcccctt
    26881 aagaataagt tgcctatttt cgtagttaac ggatccgtta atgtgaatca ttcttttatg
    26941 ttatgatttt aaaaggaatt ttatgaaaag cctctcctat aagcggatct ataaatcaca
    27001 agaatacctg gcaacgttgg gcacaattga ataccgatca ttgtttggca gttacagcct
    27061 gaccgttgac gacacggtgt ttgcgatggt ttctgatggt gagttgtatc ttcgggcttg
    27121 tgagcaaagt gcacagtact gtgtaaaaca tccgcctgtc tggctgacat ataaaaagtg
    27181 tggccgatcc gttaccctca attactatcg ggttgatgaa agtctatggc gaaatcaact
    27241 gaagctggtg cgtctgtcga aatattctct cgatgctgcg ctgaaagaga aaagcacgcg
    27301 caatacccgg gaaagactga aagatttgcc caatatgtct tttcatctgg aagcgattct
    27361 cggggaggtg gggattaagg atgtacgggc gttacgtata cttggggcaa aaatgtgttg
    27421 gttgcgactg cggcagcaaa acagtctggt gacagaaaag attctgttta tgcttgaagg
    27481 ggccattatc ggcattcatg aagctgcgct cccggtggca cgccgccagg agcttgcaga
    27541 atgggctgac tctcttacgc cgaaacagga gtttcctgcg gaacttgagt aatctggcgt
    27601 tgcagacgac caatctctgg cagtaatgca atcaataacc caacttgttg tacgaccaga
    27661 ggttctttgc tgtctgcccg tggttcaagt tgctgcatcc gctgtttcag gccagctaat
    27721 gcctgattga cgcgttcttc atcagcaggt tgatgatgta acgcgtcatc aacatagcaa
    27781 actgcgtcat caagaaacgc cagaatttca ggattagtta actgctcccg gtgagcaccg
    27841 agggctgaga tatagctggt aaacgtatgg ttaaggcaca gcaaccgaaa cgcggcttcg
    27901 cgaatttgcg gggtaacgtt cggttcgctg gacatatttg ataccaccga cgccagttca
    27961 gcatcacggt tgtgtgcatc gcggcgggca atacgatacg ccagacggtt atcacgcccc
    28021 tgatggtatt gctccagtat ggcatcgaga taccggcagt tggcctcggt ggcgcgttcg
    28081 agcattcgcg gcagattgcg aaacttccag tcaggccaga tgtagctcac tgccgcccac
    28141 gcaatggcac aaccaatcag cgtatcgatt acgcgaggta acgccacttc aaaaccttca
    28201 cccagcaagt tgaaacacag tagcaccaaa agtgtgatga acatcgttgc atgggcgtat
    28261 tgcacgttac ggaaggcaaa aaagagcacg ccggtaataa ccagcagcac cagctgccct
    28321 tccagtgatg gcacaaacca cagcacagga ataccaatcg cgatacctac cagcgtacca
    28381 ataatcctta acttcaggcg gtggcgcgtg gcgttatagt ttggctggca gacaaacaaa
    28441 cttgtcagca agatccaata cccgtgatgc attccggtta tctgaatgat ggcgtagccg
    28501 aagcacaaca ccagcgacat tcttaccgca tgacggaaga gggcggattc cggcgtgaag
    28561 tgacggctaa gacgcagcca gatatcactc aatccgtgcg ggctgtcatc agcgagctca
    28621 ttttcgtcat tattatgggg cagtgcctgg gcctgttctg attcaattgt tgccagttgg
    28681 gcatcaatgg cacgtaaatt gttcagtaaa aatcctagtg ttttgagtaa atcagcagga
    28741 gcgccgttat cgcgcatccg ctccagcgca gcatcaatat gcgtaaaagc gcgctcaaaa
    28801 tgcggatcat gttgataagg ctgacgcagc aaaatacagc gtgacagttg ctggcacgcc
    28861 tggccctgca tcgacatcag ccgctgaaaa cggaacagca cgtcgctgtg gcgaaaatgt
    28921 tcacgcaatg tttgatattg aatatgagaa gagctggcac gctcgtgaat atcctgcgcg
    28981 acaaagtaat aatgcagtgt gcgacgcgtt cctcgttgac cacgatcgcc acgtaagcgg
    29041 gtcagcagcg agagtttcgt ctgattcaat gtcgccatca gctgaccgtt ggcgagagcc
    29101 aaatcgtaca gcggtgcctg gctttcatct tcaatatcag gatcaaacat gcgcgacttg
    29161 agctcaagat aacgcgccag ttgttcatag caacgcgcca ggttgtcctg cagcgggcgg
    29221 accgggaaca gcagatgacc aataagtgtc aggacgttgt accagacggc accggccagc
    29281 agatacatcg gctgctgata ccagtgctca tacagtgacg tccccaacat agtgtaaatg
    29341 gcgatcagta acgcaccgaa ggcaattgtt gcatagcgtt gacccagacc gccgagcaaa
    29401 atgaagccgc tggtagagag cgttaaacca attgcaaata gccagggcca gggaaacagc
    29461 aattctactg aggccgaggc gataaaaaag cagaacagcg taatgatgag gttacgcaaa
    29521 cgtcccgcca gtcggtcatc gagatcggtc agcgccgctg ccaccatccc cagcgttagc
    29581 ggaatcgtca gttttacatc acccagccac cacggaaacg ctgtggttcc acaaagcgca
    29641 ataaaaatac gcgcgtaata cagccaggcg ctgttccagg tatagcgttt gagcaaagga
    29701 cttagcataa aggccatatt agtggattat tcaaaacgac gacgtgcatt ggcttcacgc
    29761 gcagcttgcg ctgtttctac cgataccacg cgacgaccga ccggccatag tgcaatagcg
    29821 gcaattttaa agttcgcaat gccgacagga atgccaatga tggaaataca ttgtgcgatg
    29881 cccgttgcaa tgtgcatcag gcataaccac cagccaaaga aaatcagcca gaaaatattc
    29941 aataccgtac cgccagtatt tagcagcaca tttttgccag ccgggttcag ttcatcgaca
    30001 tgaatagctt cattgccata aggcaccaga gacagtttag tgatctccca gcaggatcgt
    30061 gtcagcggta aggtaaaaat cagcacaata ctgaccagag tcgccaacag ccagcccaga
    30121 gtggtggcaa atccgccaag cacaaagttc agaatgttca aaacggtacg cataaaacct
    30181 cgctttactg tggttttcag taatgggcgg caattgtaac gtttttttgg gctggagcac
    30241 gttttctctg acggttacac tgataagaaa taatttcgtg tggatctaca gagtcatgga
    30301 actgaaagcg acaacgcttg gaaaacgtct ggcacagcac ccttacgatc gggcggtgat
    30361 cctcaatgcc gggattaaag tctccggcga tcgccacgaa taccttattc ctttcaatca
    30421 attactggcg attcactgta agcgcggtct ggtatggggc gagctggaat ttgtactgcc
    30481 gggcgaaaaa gtggtgcgtc tgcacggtac cgaatggggc gagacgcagc gtttttacca
    30541 tcatcttgat gctcactggc agcggtggag tagcgagatg agcgaaattg catctggtgt
    30601 tttacgccag caactggatt tgattgccac gcgtactgga gaaaataaat ggctgacgcg
    30661 tgagcaaacc tctggcgttc agcaacaaat ccgccaggct ttgtcggcgt tgccgttgcc
    30721 ggttaaccga ctggaagaat tcgataactg ccgtgaggca tggcgtaaat gtcaggcctg
    30781 gttgaaagat attgaaagcg ctcggttgca gcataaccag gcgtataccg aagccatgct
    30841 taccgagtat gcggattttt tccgccaggt cgagtcttca ccgctgaatc cggcgcaggc
    30901 ccgggcagtc gttaatggcg agcattctct gttagtgctg gctggtgcag gaagcggaaa
    30961 aacgtcggtg ctggtggccc gtgcaggctg gttgctggcg cgtggtgaag cgtcccctga
    31021 gcaaatttta ttgctggcgt ttggtcgcaa agccgctgaa gagatggacg agcgtattcg
    31081 agaacgacta cataccgaag acattaccgc acgcacattt catgcgctgg cgctgcatat
    31141 tattcagcag ggcagcaaaa aagttccgat agtcagcaaa ctggaaaatg ataccgctgc
    31201 ccgtcatgaa ctttttattg ctgagtggcg caagcaatgc agcgaaaaga aagcgcaggc
    31261 gaagggctgg cggcaatggc tgacggaaga aatgcagtgg tcagtgccag aaggtaattt
    31321 ctgggatgat gaaaaattac agcgtcgtct tgcctctcgc ctcgatcgct gggtaagtct
    31381 gatgcggatg cacggtggtg cacaggcaga aatgattgcc agtgcacccg aagtgattcg
    31441 cgatctgttc agtaaacgta tcaagttgat ggccccgtta ttaaaagcct ggaaaggtgc
    31501 gctgaaggca gaaaacgctg tcgatttttc gggccttatt caccaggcga ttgtgattct
    31561 ggagaaaggt cgctttatca gcccgtggaa gcatattctg gttgatgaat ttcaggatat
    31621 ctcgccgcag cgcgcagcgt tgttagcggc attacgcaag caaaacagtc agacgacgtt
    31681 gttcgccgtt ggtgatgact ggcaggcgat ttatcgcttc agcggtgcgc aaatgtcgct
    31741 caccaccgct ttccatgaaa actttggtga aggtgatcgc tgcgatttag acacgactta
    31801 ccgttttaac agtcgtatcg gtgaggtggc aaaccggttt attcagcaga acccaggcca
    31861 gctgaaaaag ccgctaaaca gcttaaccaa tggagacaaa aaagccgtca cgttattgga
    31921 tgagagccat cttgacgctt tgctggataa gctctctggt tatgccaaac cggaagagcg
    31981 cattctgatc ctggcgcgtt accatcacat gaggcctgcc agcctggaaa aagcggcaac
    32041 acgctggccg aagttgcaaa tcgactttat gaccattcat gccagcaaag ggcaacaggc
    32101 ggattacgtc atcatcgttg gcttgcagga ggggagtgat ggttttccgg ctgcggcgcg
    32161 ggagtcgatt atggaagagg cgctactgcc atcggttgag gatttcccgg acgctgaaga
    32221 acggcggtta atgtacgtgg cgctgacccg ggcacgccat cgggtatggg cactgtttaa
    32281 caaagagaat ccctctccat ttgtcgaaat actgaaaaat cttgatgtgc cggtggcgag
    32341 aaaaccgtaa gaaacaggtg gcgtttgcca cctgtgcaat attacttcag acggtccgcg
    32401 agataacgct gataatcggg gatcagaata tcgaccgcgt cgttgaaatg cggcgactgg
    32461 ataatgaagt ctgccgttgc cacgttggtg gcgaccggaa tgttccatac cgtcgccaga
    32521 cgcagcaagg ctttcacgtc agggtcgtgc ggcacggcgt ttagtgggtc ccagaagaaa
    32581 atcaatacat caattttccc ttctgagatc aatgcgccaa cctgctggtc accccccatt
    32641 gggccactca acatcgcgtt gacgttcatg ccggttgcac gggaaattaa gttaccggta
    32701 gtgcctgttg catacagtac gtgttgttcc agtaacggtt gatgccgttc cacccagctc
    32761 atcagcattt gtttgcagtg atcgtgtgcc accagcgcaa tatgtttccg cgcaggtaaa
    32821 gtgcgtgtcg tcagttccat aatgtacatc cgtagttaac tttcctacag attactgtaa
    32881 gcacttatcg ctgcaagata aagaccgaaa aagcctgcgc acaggcacaa aaatctcagg
    32941 aagatggttg tttttccgcc cattgcagga aagtatttcg cgtttgtggg tcagccagtt
    33001 taaaccaata cttcagccgt tgttctgtga gcacctgaga ctgcggtgga atagcatcca
    33061 gctcggaaac acccgaaagc agcgtactat catctgccat catcgtggca aattgcggta
    33121 gtgaagcgcg tttcgcggat ttgttatagc gttcaacctc gacctcataa tcaatcgttt
    33181 ttgccgttga ggtaatggcg agaatatcca gctttaccgg aatcggcgtc gcatcgccat
    33241 ccaacaattc aaggcgcggc gcggcatcaa aatggttagc ttcccgctca ttctccagac
    33301 gaggcaggcg aaaattgacc tggttgatga gctgggtatt aaaactgacc accagcggtg
    33361 gggagatata gagccgttct tcactattgg aaagatgaat cgtcttctca acgcgaaaca
    33421 ctaactgatg cggtccgtta tccagttcaa tgctgtccgc accgcgcagc agagagctgg
    33481 aaaccttctt gccgtcgagc accagaagat cgacatcggt tgaaagccgc agcgtggtgg
    33541 caaaaacgga taccggcaaa cataatgcaa tcaaggtggt cacgatgccg gttttcatag
    33601 gtagctcctg tcaaaagacc gcccgaataa taatgtgtca aaatttttct ttaatcatac
    33661 ttactaacat atagacatat ttcccgcttt tgctctcatt cattcgtatt agctgcatgg
    33721 ttggcatgtc ggctttgtcg tacactttgt taaacagtct ggaggaaata atgaaagaaa
    33781 ccgatattgc aggcatttta acgtccactc acaccattgc gctggtgggg gcaagcgaca
    33841 aacccgatcg tcccagctat cgcgtgatga aatatctact cgaccagggg tatcacgtca
    33901 ttccggtttc gccaaaagtt gctggcaaaa cactgctggg ccagcagggg tatggcacgc
    33961 tggcagaagt tcctgaaaaa gtcgatatgg tcgatgtttt ccggaattca gaagcggcgt
    34021 ggggcgtagc gcaggaagcg atcgccatcg gcgcgaaaac cctctggatg caactggggg
    34081 tgattaatga gcaagcggca gtgctggctc gggatgccgg attaaatgta gtgatggatc
    34141 gctgcccggc tattgagatc cctcgcctgg gcctggccaa ataaaaaatc cccggaaggc
    34201 aaaaaccttc cggggatttg ttcagggatt agttacgcag acgcggggcc tggagttgtt
    34261 tacggatggt ctgcgccagc tcatccatgg atggctgttc aggatgttca tcctgcaatt
    34321 cgctacttag ctgggcttca gccaggtagg tatgaaccgg taggccgtta tcgtcctcca
    34381 tcaccacgtg ataccacgga gcggcgcgaa gctcgtcatt caccgccagc tcatcaggcg
    34441 acggttcaga gagcgaataa accgggtcga tatccacgac cactccgaga taacctaaca
    34501 gggaatggcg gacctgctgg ccgataccga atttgctggc aatcatagtc acctcccggg
    34561 aaatctttac ctttgatata agggtaaaat tccacatttc aagttacatg acgcgacagg
    34621 caaacccttt cagatatagc ccttccggat aggtagcgat caccggatga tcggctgcct
    34681 gacggaactg ctctataaat tgtacatcac gaccggcatc aattgcggca tccgcgatga
    34741 ttttctgaaa taaatcgctg gtcatcagac cggaacagga gaaagtcagg agaataccgc
    34801 cttcattcag cagctgaatc gccagcatgt tgatgtcttt ataaccacga cacgcgccca
    34861 tcaactggct tttattctca acaaacttcg gcgggtccat cacgataacg tcaaattttt
    34921 caccgcgatc gcgataagta cgcagcaatt taaagacatc atcacggaca aactcagcct
    34981 tgctcagatc cagtttgttc agctcaacgt tctgccgtgc aatatccagg gcttcctggg
    35041 aggtatcaac gctgacaacc tggctgcaac cgcccatcag tgccgatacg gcgaaaccac
    35101 cggtatagga gaaacagtta agtacgcgtt tattttcgac gtagcggcgg gtagccaggc
    35161 ggctgtcacg ctgatccagg tagtagcccg ttttgtgtcc gtgctgaata ttcaccagca
    35221 gtttcatgcc gtgttcttca atcggcagca gggcaggcgg caactcgccg gtgatggggc
    35281 cctgagtcag ctccatccct tcttttttac gtaccgcgac gtcgctgcga tcgtaaatgg
    35341 cacattccgg gtacagcgtt tgcagggcgc taattaatgc cgcgcgctga tactctgcgc
    35401 cagcactcag cagttgcagc accagaaaat taccgaaacg atcgatagta atacccggca
    35461 ggccatcaga ttctccggcg attaaacgat agctgtcgag gccatctttt tgcgccagcc
    35521 agtcacgcca tttttgtgct tgctgcaaac ggcgggtaaa aaaagcaatg tcgatagatt
    35581 cattcgggtc aaacgtccag acgcgcgccc ggatttgcga agctggcgaa taagcgccgc
    35641 gtgctaacca ttttccctga tgatcaacaa tatcgatggt ttcaccgagg ctggctttac
    35701 cttccatgcg ggcaacggcc ccggaaaaga cccacggatg gcgacgaagt aatgattttt
    35761 cgcgcccttt ggctaacact aaacgtacac tcataattta cttttctgtc gatgcaaaga
    35821 aatgggcgtc attgtccgga gttttccatg gatttgcaac gcgtccatta aggataagga
    35881 gagcagcaat gtcgaaagtc tgcataattg cctgggttta cggtcgagtt cagggcgttg
    35941 gatttcgcta taccacgcag tacgaagcga aaaaactggg attagccggg tacgccaaaa
    36001 atcttgacga tggcagcgtg gaagtggttg cctgcggtga agaagggcag gtagaaaaat
    36061 taatgcagtg gttaaagagt ggcggcccgc gttctgcgcg tgtagaacgg gtgctcagcg
    36121 agccgcatca tccctcgggg gaattaaccg attttcgtat ccattaaata cattttaccg
    36181 gttttggcag gccagcaatt ttggtggctt gcttagccgg acctttcgga aacagtcggt
    36241 ataaatagcg gctgtttcct ttttcttcgc caaatttatt cgccatcgct tttaccagca
    36301 tacgaatcgc cggagaagta ttgaattcca gatagaaatt acgcacaaaa cgcaccactt
    36361 cccagtgttc tggcgacagc gtaatccctt cgttctctgc aatcaccact gccagcggct
    36421 cactccactg gctgctttct ttgagatagc cttcggtatc cgtttctatc tctttacctt
    36481 cgaagatcag cataattatt actaccttaa tcagactggc ggcagtgtaa caaaaaacaa
    36541 agccccgcat aaagcgaggc tatgaaagtg ttagcgggta agattaatcg cggctggcga
    36601 agcccagaat gctcagcagg ctgacgaaga tgttgtacag cgaaacatac aggctaaccg
    36661 tggcacgaat atagttcgtt tcaccgccat gaatgatgtt gctggtttca aacaagatag
    36721 cgccagagga gatcagaatg aagaccgcgc tgatcgccag atgcagagca ggcagttgca
    36781 ggaagatatt agcaaccata ccaatcagca ccaccacaat acccgccatc agcataccgc
    36841 cgaggaacga catatctttg cgggtggtca gcacatatgc ggagcagcag aagaacacta
    36901 acgccgttcc gcccagtgcc atagcgatta cgtcacccat tccggcagac agataggtat
    36961 tcagaattgg tccgaggata taacccagaa aaccggtaaa ggcgaatgcg gagataatcc
    37021 cggtcggctt attcgccgtt ttataggtca ggaacatcaa accatacata cccaccagcg
    37081 tcagaatcag acccggagat ggcagcatca gcaccgtact ggcagttgcg gtaatcgccg
    37141 aaaaggccag cgtcaggctc agcagaaaat aggtattacg cagcacctta tgggtgctaa
    37201 gcagcgatgt acggtcatgt gaagaactaa caatacgatc cattgagtca ctctctatga
    37261 cagatgtaat taattaagca gcataatgat aatgcgtaag ggcacccaga agttttaccc
    37321 atctttacgc atttgatctg gaacaggttt aacagcggat tatcaggtta ttaagcaatg
    37381 atataacgct ctgagaattt cgacagacaa aagaaaaagg ggttagcatt tagctaaccc
    37441 cttatcttat ttggcggaag cgcagagatt cgaactctgg aaccctttcg ggtcgccggt
    37501 tttcaagacc ggtgccttca accgctcggc cacacttccg gaatgacgcg cactataaac
    37561 atcccgatgc ggcgtgtaaa cccctaattt gtttgtttgc ctgaaaaaca gccaaaagtg
    37621 cattgatagc atgaaataac agcagattga tcatttcatc accatgaatt ccttctcttt
    37681 tactcgttta gcaaccggct aaacatcccc actgcccggc aaaaagaaaa ataggtccat
    37741 ttttatcgct aaaagataaa tccacacagt ttgtattgtt ttgtgcaaaa gtttcactac
    37801 gctttattaa cattactttc tggcgacgtg cgccagtgca gaaggatgag ctttcgtttt
    37861 cagcatctca cgtgaagcga tggtttgcct tgctacaggg acgtcgcttg ccgaccataa
    37921 gcgcccggtg tcctgccggt gtcgcaagga gaagagacgt gcgatatgaa taacgaggaa
    37981 acattttacc aggccatgcg gcgtcagggc gttacccggc gcagctttct caaatattgt
    38041 agtctggctg ccacgtcgct gggattaggc gcgggaatgg caccaaagat tgcctgggcg
    38101 ctggagaaca aaccgcgcat tccggtggta tggatccacg gtctggaatg cacctgctgt
    38161 accgaatctt ttatccgctc cgctcaccca ctggcgaagg acgtcatcct ttccctgatt
    38221 tccctcgatt atgacgatac tttgatggct gccgccggaa cccaggcgga agaagtcttt
    38281 gaagacatca tcacgcaata caatggcaaa tatatcctcg cagtagaagg taatccgccg
    38341 ctgggcgagc aggggatgtt ctgtatcagc agcggacgac cgtttattga gaaactcaaa
    38401 cgtgccgctg ccggagccag tgcgattatc gcctggggaa cctgcgcgtc ctggggctgc
    38461 gtgcaggccg cgcgacccaa tccgacgcag gcaacgccta tcgacaaagt catcaccgac
    38521 aaacccatta tcaaagtacc tggctgcccg ccgatcccgg atgtgatgag cgccatcatt
    38581 acttacatgg tgacctttga tcgcttgcca gatgtcgaca aaatgggccg tccactgatg
    38641 ttttatggtc agcgtatcca cgataaatgt tatcgccgcg ctcacttcga cgccggagag
    38701 ttcgtccaga gttgggatga tgacgctgcc cgcaaaggtt actgcctgta caaaatgggc
    38761 tgcaaagggc ctaccaccta taacgcctgt tcctccacac gctggaacga tggcgtttcc
    38821 ttcccgatcc agtcaggtca cggctgcctg ggttgtgcgg aaaatggttt ctgggatcgc
    38881 ggttcgttct acagccgcgt ggtcgatatt ccgcaaatgg gtacccattc caccgccgat
    38941 accgtcggtt taaccgcact tggcgtggtg gcagcggctg ttggtgtgca cgcagtcgcc
    39001 agcgccgttg accagcgcag acgccataac cagcaaccta cagaaaccga acatcagcca
    39061 ggcaatgagg ataaacaggc atgagcactc agtacgaaac tcagggatac accatcaata
    39121 atgccggacg ccgcctggtg gtcgacccga ttacgcgcat tgaaggccac atgcgctgcg
    39181 aagtgaatat taacgatcag aatgtgatca ccaatgccgt ctcctgcggc accatgtttc
    39241 gtgggctgga gatcatcctg caagggcgcg acccgcgcga tgcgtgggcg ttcgttgaac
    39301 gtatctgcgg cgtctgtact ggcgtacacg ctctggcttc ggtttacgcc atcgaagatg
    39361 ccatcggtat taaagtgccg gacaacgcca atatcatccg caacattatg ctggcaacgc
    39421 tctggtgcca cgatcatctg gtgcacttct atcagcttgc cgggatggac tggatcgatg
    39481 tgttagatgc gctgaaagcc gacccgcgga aaacctccga actggcgcaa agcctctcct
    39541 catggccgaa atcatcccct ggctatttct tcgacgtaca aaaccgcctg aagaaattcg
    39601 ttgaaggcgg gcagttgggg atcttccgca atggctactg ggggcacccg cagtacaaac
    39661 tgccgccaga agccaacctg atgggctttg cccactatct tgaagctctc gatttccaac
    39721 gtgaaattgt caaaatccac gcggtctttg gcggtaaaaa cccgcatcca aactggattg
    39781 tcggcgggat gccttgtgcc attaacattg acgaaagcgg cgcggtcggg gcggtcaata
    39841 tggaacgcct gaacctggtg cagtcaatca ttacccgtac ggcggatttc attaacaacg
    39901 tgatgatccc cgacgcctta gccatcggtc agttcaacaa gccgtggagc gaaatcggca
    39961 ctggcctttc ggataaatgc gttctcagtt acggcgcgtt cccggacatc gccaacgact
    40021 ttggcgagaa aagtctactg atgcctggcg gcgcggtgat taacggcgac ttcaacaatg
    40081 tgctgccagt ggatttggtt gatccgcagc aggtgcagga gtttgtcgat cacgcctggt
    40141 atcgttatcc caacgatcag gtcgggcgtc atccgttcga tggcattacc gacccgtggt
    40201 acaaccccgg cgatgtcaaa ggcagcgata ccaacattca gcagctgaat gaacaggaac
    40261 gctactcgtg gatcaaagcg ccacgctggc gcggtaatgc gatggaagtg gggccgctgg
    40321 cacgcacgtt aatcgcttat cacaaaggcg atgcggcgac cgttgagtcg gtcgatcgca
    40381 tgatgtcggc gttgaacctg ccgctttccg gcatccagtc aacattaggc cgcattttgt
    40441 gtcgcgcgca cgaagcgcag tgggccgcag gcaagttgca atatttcttc gacaagctga
    40501 tgaccaatct caaaaacggc aatctcgcca ccgcctccac tgagaagtgg gaaccagcaa
    40561 cctggccgac agagtgccgt ggtgtcggct ttaccgaagc gccgcgcgga gcgttaggcc
    40621 actgggccgc cattcgcgat ggcaagattg atctctacca gtgcgtggtg ccgaccacct
    40681 ggaacgccag cccgcgcgat cctaaagggc aaattggcgc ttatgaagcg gcgctgatga
    40741 acaccaaaat ggcgatcccc gagcaaccgc tggagatcct gcgtactctg cacagctttg
    40801 atccgtgcct cgcctgttca acacacgtgc tgagcgacga cggtagcgag ctgatctccg
    40861 tgcaggtgcg ttaacagcga aggagaatca tcatgcaaca gaaaagcgac aacgttgtca
    40921 gccactatgt ctttgaagcg ccagtgcgta tctggcactg gctgacggtg ttatgcatgg
    40981 cggtgttgat ggtcaccggt tactttatcg gcaagccgct accttccgtc agcggcgagg
    41041 cgacgtatct gttctatatg ggctacatca ggttaattca cttcagcgcc gggatggttt
    41101 ttaccgtggt cttgctgatg cggatctact gggcttttgt cggcaatcgc tactcacgcg
    41161 agctgtttat cgtgccagta tggcgtaaaa gctggtggca gggcgtgtgg tatgaaatcc
    41221 gctggtatct gtttctggca aaacgtccga gtgccgatat aggtcataac cccatcgccc
    41281 aggcggcgat gttcggctat ttcctgatgt cagtttttat gatcatcacc ggctttgcgc
    41341 tgtacagcga acacagccag tacgctattt ttgcgccgtt ccgttatgtg gtggaatttt
    41401 tctactggac gggcggcaac tcaatggacg ttcacagctg gcatcggctg gggatgtggc
    41461 tgattggcgc gtttgtgatc ggtcatgtct acatggcgct gcgtgaagat atcatgtccg
    41521 acgacacggt gatctccacc atggtcaacg gctaccgtag ccacaaattt ggcaaaataa
    41581 gtaacaagga gcgttcatga gcgagcaacg cgtggtggta atggggctgg gcaacctgct
    41641 gtgggccgat gaaggcttcg gcgtgcgggt ggcggaacgg ctgtatgccc attaccactg
    41701 gcccgagtat gtggagattg tcgatggcgg tactcaggga ctgaacttgc tggggtatgt
    41761 cgaaagcgcc agtcacttgt tgattctcga tgccattgac tacgggctgg aaccaggaac
    41821 gctgcgaacc tatgccggag aacgcattcc ggcttatctc agcgcgaaga aaatgagcct
    41881 gcaccagaac agcttctccg aagtgctggc gctggcggat atacgtggtc atttgccagc
    41941 acatattgcc ctcgtcggtc tgcaacccgc aatgctcgac gactacggcg gtagcctgag
    42001 cgaactggcg cgggagcaac tgcccgctgc ggaacaatcg gcgctggcgc agctggctgc
    42061 gtggggaatt gtgccgcaac cggctaagga atcgcgctgt ctcaattatg actgtctgtc
    42121 gatggaaaat tacgaaggcg ttcgcttgcg tcagtacagg atgaggctgg aggaacaggg
    42181 atgagcaacg acacaccatt taatgcgttg tggcaacgaa tgctggcgcg cggctggacg
    42241 ccggtcagtg aatgccgtct tgacgactgg cttacgcaag cgccagacgg cgtggtgtta
    42301 ttaagtagtg acccgaaacg cacgccagaa gtcagcgata acccggtaat gattggcgaa
    42361 ttactgcgcg agtttcccga ctatacatgg caggtagcga ttgctgatct tgagcagagc
    42421 gaagccatcg gtgatcgttt tggcgtattt cgttttcccg ccacactggt gtttaccggt
    42481 ggtagctatc gcggcgtgct gaatggtatt cacccgtggg cggaactgat aaacctgatg
    42541 cgtgggcttg tcgaaccaca acaggagcgt gcctcatgag cgaaactttt ttccatctgt
    42601 tggggccagg aacgcaaccg aacgatgaca gtttcagcat gaatccactg ccgatcacct
    42661 gtcaggtgaa tgatgaaccg agtatggcgg ccctggagca atgtgctcac agcccgcagg
    42721 tgattgcgct gttaaacgag ttacaacaac aaataagcga acgccagcca ccgttgggcg
    42781 aggtgctggc agtcgatctg ttaaatctca acgccgacga tcgtcacttt atcaatacgc
    42841 ttctcgggga aggggaagtg tcagtgcgca tacagcaggc tgacgacagt gaaagtgaaa
    42901 tacaggaggc gatcttctgc ggattatggc gggtgcgcag acgtcgcggc gacaagttgc
    42961 tggaggacaa actggaggct ggctgcgcgc cgctggcatt gtggcaggcg gcaacgcaaa
    43021 acgtcttgcc gacagattcg ctgttaccgc cgcccattga tggcctgatg aatggcctac
    43081 cgttggcgca tgagctactg gcgcatgtac gtaaccccga cgcgcagccg cacagcatta
    43141 atctgacgca attacccatc agcgaggctg atcggctttt tctctcacgt ctctgtgggc
    43201 cgggaaatat tcagattcgc accattggct atggcgagag ctatatcaac gccacggggt
    43261 tacgccatgt ctggcattta cgctgcacgg acaccttaaa aggcccgtta ctggaaagtt
    43321 atgaaatctg cccaataccg gaagtggtgc tggcagcgcc agaagatttg gtcgactctg
    43381 cgcagcggct tagcgaggta tgccagtggc tggcggaagg tgcaccgacg taaaaagacg
    43441 gtaagtatcg ctttcaggct tatgaatatc gcaatcggcg aatacctctg gtcgtagagt
    43501 ttcaggataa agagggagat ctaccattaa cgggttattt ttctctcttc gcctacagga
    43561 gtgcgcatgt gggatgtcat tgatttatcg cgctggcagt ttgctctgac cgcgctgtat
    43621 cactttttat ttgtacccct taccctgggg ctgatttttt tgctggctat catggaaacc
    43681 atttacgtgg tcaccggcaa aacaatctac cgcgatatga cgcgcttctg gggtaagctc
    43741 ttcggtatca actttgctct tggcgtggct accggcctga cgatggagtt tcagtttggt
    43801 actaactggt cattctattc caactatgtg ggcgatattt ttggcgcacc gctggcgatg
    43861 gaagcattaa tggccttctt cctcgaatcc acctttgtcg ggctgttctt cttcggctgg
    43921 cagcggctga ataaatacca gcacctgctg gtgacgtggc tggtggcgtt cggttcaaat
    43981 ctctctgcat tgtggatatt gaatgccaac ggttggatgc aatacccgac cggtgcgcat
    44041 tttgatatcg acaccctgcg tatggagatg accagtttca gcgagttggt ctttaacccg
    44101 gtcagccagg tgaaatttgt gcacactgta atggcgggct acgtgaccgg ggccatgttt
    44161 attatggcga tcagcgcctg gtatttactg cgcggacggg agcgcgatgt tgcattacgc
    44221 tcgtttgcca tcggttccgt cttcggtact ctggcgatta tcggcacgct gcaactcgga
    44281 gacagttctg cgtatgaagt cgcgcaagta caaccggtaa aactggcggc gatggaaggg
    44341 gagtggcaaa cggaacctgc acctgcaccg ttccatgtgg ttgcctggcc ggaacaggat
    44401 caagagcgta acgcctttgc tatcaaaatt cccgcgctgc tagggatcct cgccactcac
    44461 tcattagata aacccgtgcc gggtctgaag aatttgatgg ctgaaaccta cccacgcttg
    44521 caacgcggac gtatggcctg gctgttaatg caggaaatat cgcaaggcaa tcgtgagccg
    44581 catgtgttgc aggcatttcg ggagctggaa ggcgaccttg gttacggcat gttgctctcc
    44641 cgctatgcgc cggatatgaa ccatgtcaca gctgcacagt accaggcggc gatgcgtggc
    44701 gcgatacctc aggttgcgcc ggtattctgg agtttccgca tcatggtggg ctgtggttct
    44761 ctgctgctac tggtgatgct gattgcgctt gtccagacgc tgcgtggcaa aattgaccag
    44821 catcgctggg tgctgaaaat ggcgctctgg agtttgccgc tgccgtggat tgcgattgaa
    44881 gccgggtggt ttatgaccga gtttggtcgt cagccgtggg cgatacagga catcttaccg
    44941 acatactccg cgcactccgc cttaaccaca ggacaactgg ctttctcact gatcatgatc
    45001 gtagggcttt acaccctgtt cttaatcgcc gaagtctacc tgatgcagaa atatgcccgt
    45061 ctggggccga gcgcgatgca gagtgaacaa ccgacgcagc aacaggggta aaggagaaaa
    45121 tcatgtttga ttatgaaaca ttgcgcttca tctggtggct gctgattggc gtgatcctgg
    45181 tggtctttat gatctccgac ggatttgaca tggggatcgg ctgtctgctg ccgctggtgg
    45241 cgcgtaatga tgatgaacgt cggatagtga taaacagcgt tggcgcgcac tgggaaggca
    45301 atcaggtctg gttgatcctc gcaggtgggg cattatttgc cgcctggccc agagtgtatg
    45361 cagcggcgtt ttccggcttt tatgtggcga tgattctggt gctgtgctcg ctgttcttcc
    45421 gcccgctggc ctttgattat cgcggaaaaa tcgccgatgc acgctggcgt aaaatgtggg
    45481 atgccggact ggtcatcggc agtctggtgc cgccggtagt cttcggtatt gcattcggca
    45541 acttgctgct cggcgtgccg tttgctttca caccgcaatt acgcgtggaa tatctcggca
    45601 gcttctggca actgctgacg ccattccctt tattgtgtgg attgctcagt cttgggatgg
    45661 tgattttgca aggtggcgtc tggttacaac tgaaaactgt tggtgtgatt catctgcgtt
    45721 cacagctggc gaccaaacgc gccgcactac tggtgatgct gtgctttttg ctggcgggtt
    45781 actggctgtg ggttggtatt gatggctttg tactgctcgc ccaggatgct aacggtcctt
    45841 ccaatccgtt aatgaaactg gtggcagtgc tacctggtgc ctggatgaat aatttcgtcg
    45901 aatcgcccgt tttgtggatc ttcccgctgc tggggttctt ctgcccattg ctgacggtga
    45961 tggcgattta tcgtggtcgc ccgggttggg gatttttgat ggcgtcattg atgcaatttg
    46021 gcgtgatttt cacggcaggc atcacgctgt tcccctttgt catgccgtca agcgtgagtc
    46081 cgatctccag cctgacgttg tgggacagca cttccagtca gctgacgctg agcattatgt
    46141 tggtaatcgt gctgatattt ttgcctattg tgttgctcta cactctctgg agctactaca
    46201 aaatgtgggg acgcatgacg acagaaactc tccgccgtaa cgaaaacgag ctgtactaag
    46261 gagcaggaac aatgtggtat ttactttggt tcgtcggcat tttgttgatg tgttcgctct
    46321 ccacccttgt gttggtatgg ctggacccgc gtctgaaaag ttaacgaacg taagcctgat
    46381 gtggtgcatt agcatcgcat caggcaatca ataatgtcag atatgaaaag cggaaacata
    46441 tcgatgaaag cgatcttaat cccattttta tctcttctga ttccgttaac cccgcaatct
    46501 gcattcgctc agagtgagcc ggagctgaag ctggaaagtg tggtgattgt cagtcgtcat
    46561 ggtgtgcgtg ctccaaccaa ggccacgcaa ctgatgcagg atgtcacccc agacgcatgg
    46621 ccaacctggc cggtaaaact gggttggctg acaccgcgcg gtggtgagct aatcgcttat
    46681 cttggacatt accaacgcca gtgtctggtg gctgacggat tgctggcgaa aaagggctgc
    46741 ccgcagcctg gtcaggtcgc gattattgct gatgtcgacg agcgtacccg taaaacaggc
    46801 gaagccttcg ccgccgggct ggcacctgac tgtgcaataa ccgtacatac ccaggcagat
    46861 acgtccagtc ccgatccgtt atttaatccc ctaaaaactg gcgtttgcca actggataat
    46921 gcgaacgtga ctgacgcgat cctcagcagg gcaggagggt caattgctga ctttaccggg
    46981 catcggcaaa cggcgtttcg cgaactggaa cgggtgctta atttttcgca atcaaacttg
    47041 tgccttaacc gtgagaaaca ggacgaaagc tgttcattaa cgcaggcatt accatcggaa
    47101 ctcaaggtga gcgccgacaa tgtctcatta accggtgcgg taagcctcgc atcaatgctg
    47161 acggagatat ttctcctgca acaggcacaa ggaatgccgg agccggggtg gggaaggatc
    47221 actgattcac accagtggaa caccttgcta agtttgcata acgcgcaatt ttatttacta
    47281 caacgcacgc cagaggttgc ccgcagtcgc gccaccccgt tattggattt gatcatggca
    47341 gcgttgacgc cccatccacc gcaaaaacag gcgtatggtg tgacattacc cacttcagtg
    47401 ctgtttattg ccggacacga tactaatctg gcaaatctcg gcggcgcact ggagctcaac
    47461 tggacgcttc caggtcagcc ggataacaca ccgccaggtg gtgaactggt gtttgaacgc
    47521 tggcgtcggc taagcgataa cagccagtgg attcaggttt cgctggtctt ccagacttta
    47581 cagcagatgc gtgataaaac gccgctgtca ttaaatacgc cgcccggaga ggtgaaactg
    47641 accctggcag gatgtgaaga gcgaaatgcg cagggcatgt gttcgttggc aggttttacg
    47701 caaatcgtga atgaagcacg cataccggcg tgcagtttgt aatgcataaa aaagagcatt
    47761 cagttatctg aatgctctga ggctgatgac aaacgaagaa ctgtctaatg cgtagaccgg
    47821 aaaaggcgtt cacgccccat ccggccacct taccttcagt tttactcttt ctcggagtaa
    47881 ctataaccgt aatagttata gccgtaactg taagcggtgc tggcgcgttt aatcacacca
    47941 ttgaggattg cgcctttaat attaacgcca gcctgttcca gacgctgcat tgacaagctc
    48001 acctctttgg cggtgttcaa gccaaaacgc gcaaccagca ggctggtgcc gacagagcgt
    48061 cccacgaccg cagcatcgct caccgccagc atcggcggcg tatcgacaat caccagatcg
    48121 taatggtcgt tcgcccattc cagtaattga cgcatccgat cgcgcatcag cagttcagac
    48181 gggttaggtg gcacctgacc gcgagtaatc acatcaaagc ctcctttgcc aaaatgctgg
    48241 atcactttgt tgagctcatc tttacctgcc agatattccg acaagccatg ttcattactc
    48301 acggtaaaca ggttatgcga ataaccacgg cgtaagtcgg catcaataaa taacactttt
    48361 tgatcggact gggcgatcac cgctgccaga gttgaactga cgaacgtttt accactgtct
    48421 ggcgtcgcac cggtgatcat cagaatgtta ttctctgtct ccatcatagc gaagtgcaga
    48481 ctggttcgta gcgcacgtac ggcctccaca gcagaatccg ccgggttatc caccgccagg
    48541 aaggggatat ttttagtacg atggcgctgc tgattagaaa ataaattttt cttacgcaga
    48601 cgggtacgtt tatccagcca ctcggacatt gggatagtgg cataaacgct gatgccgtgc
    48661 tcttccagtt gttccggggc ttctacacca cgacgcaaca tcgcacgcgc cagcacggca
    48721 cccaccgaaa taaacaggcc aagaataaaa ccaagcacca cattcaacgc ttttttcggt
    48781 ttcactggct gcggctgagt gactgccggg tcgataatcc gcacgttacc aatcgcactg
    48841 gatttcgaaa tactcaactc ctgctggcgg ttaagtaatt gcagatatac cgcacggccc
    48901 gcttctacgt cacgacttaa acgcaacact tcctgttggg tggaaggcat tgccgatacc
    48961 cgcttattca ggcgtttgcg ttcttgctcc agtgtctggc gtttttccag cagcgcacga
    49021 taagttgggt gatctttctt atacagctgg gagatctctg cctcgcggaa agtcagctca
    49081 ttgagttgat tatcaacgtt cacaatctgc tcaagaacgg ctttggcttc caggttaagg
    49141 tcaaccgaat cgcgctgttg gcgataaacg ttgagttttt cttccgcctg gtccagctcg
    49201 ctgcgcactt ctggcaactg gcgctgtaag aattcaaggc tttgtgaatc ctgcgccgcc
    49261 tggcgagcga tattctgttg caaatagttg ttagcgatgc tgttcagaat acgagtaatc
    49321 aactggggat catcaccggt catggtaagt tccagcatcc cgctttcttt actgcgttcg
    49381 ctaacggtaa aggtttcctg caacgcgtta atcgcttcca gttcggtacg ctggctcagg
    49441 acaaactgcg ttcctggttt ggccttaatg tccgcgatag tcagcgcaac gccgtctttt
    49501 tccagacgct ggccgaccat accattgacg gtgaaccctt caccttccag cgtatagtgg
    49561 ccgttttccc caactgtgag tgtcagttgc tgatcctgac cattcagttg tgggatgtgc
    49621 atccagctga tcgccagctc acctggtttc tctttggtta atctcgccca gccgcgaccc
    49681 acaatcggaa aatacttctg ctcaaccatg tcgcgcagat tcagttcagc aatggtttta
    49741 ccgagaatca tgcgcgattg cagcagttgg atctccggtg ctgactcggg cgatgagtta
    49801 gggatcatat cgctcaggcc gctgagaatg gcgttgccct gtttctgctc aacctggacc
    49861 agagtatctg cctgataaat tggcgtgctt aacagcgagt aagcgacagc gatcagcgtg
    49921 aacaacgcgg tcacgctgac aataaactta cggtgatccc ataactcgcc gaccagacga
    49981 agcagatcga tctcattttc ctgagtgctg cctggtggcg tattcatatt tttagttgtc
    50041 atcgtatttt ataaactcaa attaccggct gaggcgcttc gcccattcct gactggcgcg
    50101 ctccaacata ccgtagacat gctcaaatgc gtcctgactt ttacgatagg gatccgggat
    50161 ctctttctgt tccagccatt gcccaaacag cattgtcttc ccgcggacct cgggagcgat
    50221 tgccgtaacc tgagcaatat gttccgactc cattgccaga atcagatcgt aatttcgtgc
    50281 catctcagca gtgagcttac gtccggcatg tccttcaaga gatacgccat gattagcggc
    50341 aacatctgcc gctgtcgcat ctgcagggtg ctttaccaga ccatgaacac ctgccgattt
    50401 gacttttacg cctggcagac gtttacgcag caagcgctcg ccaatgggcg aacggcaaat
    50461 gttgccggta caaaccacca ggattgagtt aaattttagt tgggccatgt ttgaatccac
    50521 cgtgaagtct ctgtcaggtc atgcaccccg gaaatcgtcg gtaccagtag agaaatcaca
    50581 cggttccaac gagccagcgg cgcagtagtg acataaacaa tgtcgtaagg ttcaagctgg
    50641 aattccgttc cgagtatcat tgccgatgcg tccttcgcat tcagctgata aatattggcg
    50701 attttgccat tctgcttgtt ttgcgttgca cgaataacaa aaatccccgt cgcatcagcg
    50761 acatcctgat tcataccctc agcgttaccc agcgcttccg ccagggtcat accgctgcgg
    50821 tccattttca gcgtgctctg tttacccact tcacccatca cgaagacttt cagatcatcg
    50881 ttgcgcggaa taaataaaat atcgcccggg tgcagcagtt tgttctggcg caagtcgcca
    50941 cgctgcatca gcgcatagag gttcactttg gtctttacgc cattctgggt gagcaccacg
    51001 ttgcgccagt ctgcatctgc ggtcagcccc cccgcggcgt taatcgcatc catgatggtc
    51061 agaggaatat tggtaatcgg ctgctgaccc gatttgctga cttcaccggt gacgtacgct
    51121 ttttgcgagc ggaacgccgc gacgctgaca tcgacctgcg ggctttcaat gactgaatcc
    51181 agacgcgctg taatctcgtt gcgtacctgg gtaagcgttt tcccggccac tttcaggcga
    51241 ccaatatagg ggtagaaaat tgcaccatca gcgttaaccc agttgccggt atcactggcg
    51301 ctacggtatt gccctgcggg tgtggttagt tccgggtgat cccagaccgt aaccatcaac
    51361 acatcaccaa tgccgatacg atattcatag ttcgcgattt gctgctccag ctccgggtta
    51421 gcctgcgaca taatcggttt ggcgcgcagc tgatcaatca atcctggcgt gacgggatag
    51481 atattgacca ttttgtccag gtcatactga ttgtcaggta gttcaatgac atccttgtta
    51541 ctggtcgata aattctgacc aggaacaagg gtacatgctg tcaaagacag aacagccaat
    51601 gtcagtaccg aaaacttaaa aatgttcttt ttcattggtt tatatttatc agtcattgta
    51661 atgttatgaa ttacgtcagg ttattgtcca accggtactt ctcgctctgc cgtcattgga
    51721 tagagctggt atttgcgtga cagcatttgt ccaccatcac gcgtcaatgg tgtccacgaa
    51781 accaccgcgc ggttgcggtt aggtccaatg gtcatcaagt cgaacggaat cgagatataa
    51841 aaacctttac taaagccgcc ttcgccgtag tcatcttttg atacgttact gattgccgcc
    51901 cataccccta ctgccacgcc gctgtcaaag cgtttggcga cgtcgatcgt tgcaccttta
    51961 tcttttgcca gatattgccc aacgctaagt ttcatcagta cgccattgag cgtcggcggg
    52021 ttccagtaag ccgtcacgaa acctgttggc gtagaataat cggtgaagcg catcatgttg
    52081 tcccagtcac gctgcttcac gtagttaacg tccacaccca gcgcccagct ggcatctagc
    52141 gggcgataaa gcagctcgga accgacaccg gcatacatcg tttccagata accgccatac
    52201 acctggccat agaaaccgtt gcctaagtcg gcaaagtagt tcgcttgcaa gttgttgaga
    52261 taaacgtcat tgcgaacgta atcacggata tgcgtgcgca cgcggggcag ggtagagtcc
    52321 gcgggtaata gcgaagactt aaacttgtcg tagttattgt aaatattggt gaaaataccg
    52381 ccatcaagca gcaggtggtc ggtaaaccag tagcgggcgc tggacatcag ccccagctgg
    52441 aacatataga aatcttccgg gccgcccagc gactgtgaaa gtgttgggtt gaaagagtag
    52501 ctaaagcgat cttcacgaat acggtagccc cgaccaaagg cagaaagatc ttcagcttca
    52561 acacgttgtt gttgcagtgg ctctgattga ccaggcgctg taccggccag ttgtttgcgc
    52621 aggctggcta cgtcggtttc ggtagtcacc atcgccatat gctcgcgctt ttgcgtcacg
    52681 ctaatcttct caacgccttg cggcaggtta ttcaccagaa tccggttggc acgatcgacc
    52741 gcttcgcgag agtcacggta tttgtattgc tgaccagaca tatacagcgt cttatcgcgt
    52801 agctgaattt ctggcgcgtc aaagcccgcg ttatacttca gggcggtaag ttggtttgct
    52861 accgtggtgt actgcaatcc ttcagattcc ggcgcaggtt gatatgccgg ttttggcgta
    52921 tcgcgcaggg cagggcgcag atcgttgaaa ttggtccgta acgtgaagcc aaacatcaac
    52981 gtgttaccgc gttcataact caggttgaga tctgcccagc tggcagcgcg atagactgcg
    53041 ccgacgttga aatggcttgc ctgaggcagt ttgcccgcaa aatcattctg gtaattgttg
    53101 ccatcgtatt cgagtttcag acgcagagga ttccacggcg tttgatactc aataccgcca
    53161 aaaatagagg ctggaccacg aaagatatcg ctaaagctga tatctcccgc atcgtgagac
    53221 tctgcgcgat ggcaatattt atcgcttacc cgacaaaacg ggttggtaat attgcccgca
    53281 ttaccggcgt agccccaggc catcccgagg gtgaaatcga acgggcccgc ttgcttgctg
    53341 gccaccaaat attcaccgtc aaacagaccc gtaccagcaa tatcacgttt accaaacgcc
    53401 acttgcggta gccagtaacc ttcttcccac aggcgaagtt taaaatcgaa agatttgtct
    53461 ttgtatgact gatcgccgct gaaatcttcc cactggctat atttgcgtgt gcgcacatcc
    53521 gtataacgaa tggtgccttc cagccatggg aacagcgcca cggaggtgga gtagaaacgg
    53581 tattgatcgt tatcccggta gtttacgctg aattcacctt ccggtgcgat gcgcgcattt
    53641 ggcatctgca acaatcctgt gccgccaaag tctgattgcg acgaacccag cggatccgga
    53701 taggttaata cttcagcatg acaggcactg gagacggcaa tggccaggca gcttaaaaga
    53761 taagagttct tcttcatcag tcaggaacac gttgcgttaa aacagacaca atctggtcat
    53821 ttaaatcagc atatttttcg ggcagtacat gcgcagaaaa gcctaaccaa agctggctgc
    53881 caggcggtgg ttccacatgt cttttattcc acagggcaac aggggcgacg acagcctcac
    53941 cctcaggcgt aatcaccata acgttattct tgtccgcgcc tgcaagacgg gtgtgatctt
    54001 gcagataatc ggtaacgcta cggcctgcca gccagggcaa ttgcccagcc ccagaaaccg
    54061 cacctagcag agtaatagtc accggacgct gtacggtata gagcgtatag tccccgacca
    54121 gcgggggatt gctgttctca tccactcgta caaaatcagg gtccagtttg accggtaagc
    54181 gaccagtgat attgagattg agtaactgct ggcgcacgga tttaatcgtt gccaccacat
    54241 cgtcgtcagc ttctgcttcc caggaagcaa gctgtgccat gacatgttgg tagtctttaa
    54301 gcgctttagc tttcgccgca gagtcggtca gcaaggcacc aggccaccag aggcgatcgc
    54361 gcagttgcgg ttgtgtcacc aactggacaa cattttccac ggggccaacg gaaagtgtct
    54421 gttgttcgcc aggcagataa atagtcaccg ttccttgcgc aaaggcatgg ggtgtcatta
    54481 cgtaaagtac gctggcaatg aaatacgact gtaatttatt catggtgcgg gtttaagaaa
    54541 cgtcatttct acgggaataa cccctgcgcc taacatttgc cgactttgac gaacctggcc
    54601 agtagccgaa tcgacccaga atgtgttttg ccactgtttt tctggcagcg tggaagtcac
    54661 ctcttcctgc cagacattac acagcacatc gcgacccgca atattcagcg tctctaatcc
    54721 agcaaaggaa aaagtggaac tcagggtcgc agagcgaaaa tggttgtctt cactccagag
    54781 aatatcgcgc gtccagcggc tgccgtcttt tattgccagc gcgttacgca gagggtcctg
    54841 cccggaattt gtcacttcca gcagattatt attaagtttg acggttttta aaagacgtcc
    54901 attgtgggta accagcatgg cgttatcctg ggataaccat ttgctttgtt cttgttctat
    54961 atatcccagt accacaaaga ttcgttgccc ttcattaagg cgtaaataca tcgtggaata
    55021 aggcaacgcc tggatctgct gatccgctac ggtgatatcc tgattatcga aaaggctggc
    55081 gcgaaatgta tcgaccatac tttgctggct gtgcgtacat cccgcaagaa atagtgcgaa
    55141 aatcgataaa ataagagggc gcacctttaa tccttgaaag gttaattaaa aaaatgcaaa
    55201 tctctaaagc ccggtacgaa tatcgcaacc gggtatcatt taactttaat tttgacgcgt
    55261 taaagcagta ttagcgcgta gtggacgtgg tggttgaggt gttatgagaa ccgccatcgc
    55321 cactgtcatt tgctgcaact actgccaacg tcgccacacc aacagccgtt gccacaccga
    55381 caaccgtcgc cgtgccgaca gcgccagcag ccacgccacc agtggtagca gcagtcgtgg
    55441 ttgcacctgt agtggtcgtt gtggtgccag tagtacccgt cgttgcctca gtcccgtttg
    55501 ctgcttcagg ggcagcaaat gcagctggcg tcgtcaacat gaaggccata aggattgcag
    55561 aaagcttgtg tttcataact tttcctttat tcatcgcatg gacaatacgt acgcttgaga
    55621 tcaggattat ctactttgag tataacatcc ttgtgaaatc tccctttcct cataccgcca
    55681 gcactatata ctgcgggtgg gaaaggcgcg cagagggaaa tataagattg ttaactattc
    55741 ccctctggaa atacgacttg tgagcataag ataaatctta gcaaaataca aattaa
//
LOCUS       DAZFLD010000007        50543 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.007, whole genome shotgun sequence.
ACCESSION   DAZFLD010000007 DAZFLD010000000
VERSION     DAZFLD010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 50543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50543
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.007"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            45..677
                     /gene="comR"
                     /locus_tag="ACHKBL_000360"
     CDS             45..677
                     /gene="comR"
                     /locus_tag="ACHKBL_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family copper-responsive transcriptional
                     repressor ComR"
                     /protein_id="HFO5817285.1"
                     /translation="MATDSTQCVKKSRGRPKVFDRDAALDKAMKLFWQHGYEATSLAD
                     LVEATGAKAPTLYAEFTNKEGLFRAVLDRYIDRFAAKHEAQLFCEEKSVESALADYFA
                     AIANCFTSKDTPAGCFMINNCTTLSPDSGDIANTLKSRHAMQERTLQQFLCQRQARGE
                     IPTHCDVTHLAEFLNCIIQGMSISAREGASLEKLMQIARTTLRLWPELVK"
     gene            complement(739..1278)
                     /gene="ycfJ"
                     /locus_tag="ACHKBL_000361"
     CDS             complement(739..1278)
                     /gene="ycfJ"
                     /locus_tag="ACHKBL_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HFO5817286.1"
                     /translation="MNKSMLAGIGIGVAAALGVAAVASLNVFERGPQYAQVVSATPIK
                     ETVKTPRQECRNVTVTHRRPVQDENRITGSVLGAVAGGVIGHQFGGGRGKDVATVVGA
                     LGGGYAGNQIQGSLQESDTYTTTQQRCKTVYDKSEKMLGYDVTYKIGDQQGKIRMDRD
                     PGTQIPLDSNGQLILNNKV"
     gene            complement(1505..2809)
                     /gene="ndh"
                     /locus_tag="ACHKBL_000362"
     CDS             complement(1505..2809)
                     /gene="ndh"
                     /locus_tag="ACHKBL_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017384716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone dehydrogenase"
                     /protein_id="HFO5817287.1"
                     /translation="MTTPLKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHS
                     HLWKPLLHEVATGSLDEGVDALSYLAHARNHGFQFQLGSVIDIDREAKTITIAELRDE
                     KGELLVPERKIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLK
                     YSANLGANGKVNIAIVGGGATGVELSAELHNAVKQLHSYGYKGLTNEALNVTLVEAGE
                     RILPALPPRISAAAHSELTKLGVRVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGI
                     KAPDFLKDIGGLETNRINQLVVEPTLQTTRDPDIYAIGDCASCPRPEGGFVPPRAQAA
                     HQMATCAMNNILAQMNGKPLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRI
                     ARFVYISLYRMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH"
     gene            complement(3209..3751)
                     /gene="ycfP"
                     /locus_tag="ACHKBL_000363"
     CDS             complement(3209..3751)
                     /gene="ycfP"
                     /locus_tag="ACHKBL_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase YcfP"
                     /protein_id="HFO5817288.1"
                     /translation="MIIYLHGFDSNSPGNHEKVLQLQFIDPDVRLISYSTRHPKHDMQ
                     HLLKEVDKMLQLNVDERPLICGVGLGGYWAERIGFLCDIRQVIFNPNLFPYENMEGKI
                     DRPEEYADIATKCVTNFREKNRDRCLVILSRNDEALNSQRTSEELHHYYEIVWDEEQT
                     HKFKNISPHLQRIKAFKTLG"
     gene            complement(3774..4799)
                     /gene="nagZ"
                     /locus_tag="ACHKBL_000364"
     CDS             complement(3774..4799)
                     /gene="nagZ"
                     /locus_tag="ACHKBL_000364"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HFO5817289.1"
                     /translation="MGPVMLDVEGYELDAEEREILAHPLVGGLILFTRNYHDPAQLRE
                     LVRQIRAASRNHLVVAVDQEGGRVQRFREGFTRLPAAQSFAALLGMEEGGKLAREAGW
                     LMASEMIAMDIDISFAPVLDVGHISAAIGERSYHSDPEKALAIASRFIDGMHEAGMKT
                     TGKHFPGHGAVTADSHKETPCDPRPQAEIRAKDMSVFSSLIRENKLDAIMPAHVIYSD
                     VDPRPASGSPYWLKTVLRQELGFDGVIFSDDLSMEGAAIMGSYAERGQASLDAGCDMI
                     LVCNNRKGAVSVLDNLSPIKAERVTRLYHKGSFSRQELMDSARWKAISARLNQLHERW
                     QEEKAGH"
     gene            complement(4810..5634)
                     /gene="thiK"
                     /locus_tag="ACHKBL_000365"
     CDS             complement(4810..5634)
                     /gene="thiK"
                     /locus_tag="ACHKBL_000365"
                     /EC_number="2.7.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019165 - thiamine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine kinase"
                     /protein_id="HFO5817290.1"
                     /translation="MPFRSNNPITRDELLSRFFPQFHPVTTFNSGLSGGSFLIEHQGQ
                     RFVVRQPHDPDAPRFAFLRQYRALSQLPACIAPKPHLYLRDWMVVDYLPGEVKTYLPD
                     TNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRRLKRLRKAREPRP
                     LRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDIALELAAVWVENTDQHRQLVNDYA
                     TRAKIYPAQLWRQVRRWFPWLLMLKAGWFEYRWRQTGDQQFIRLADDTWRQLLIKQ"
     gene            complement(5615..6256)
                     /gene="lpoB"
                     /locus_tag="ACHKBL_000366"
     CDS             complement(5615..6256)
                     /gene="lpoB"
                     /locus_tag="ACHKBL_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HFO5817291.1"
                     /translation="MTKMSRYALITALAMFLAGCVGQREPAPVEEVKPAPEQPAEPQQ
                     PVPTVPSVPTIPQQPGPIEHEDQTAPPAPHIRHYDWNGAMQPMVSKMLGADGVTAGSV
                     LLVDSVNNRTNGSLNAAEATETLRNALANNGKFTLVSAQQLSMAKQQLGLSPQDSLGT
                     RSKAIGIARNVGAHYVLYSSASGNVNAPTLQMQLMLVQTGEIIWSGKGAVSQQ"
     gene            complement(6270..6647)
                     /gene="ycfL"
                     /locus_tag="ACHKBL_000367"
     CDS             complement(6270..6647)
                     /gene="ycfL"
                     /locus_tag="ACHKBL_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HFO5817292.1"
                     /translation="MRKGCFGLVSLALLVLVGCRSHPEIPVNDEQSLVMESSLLAAGI
                     SAEKPVLSTSDIQPSASSTLYNERQEPVTVHYRFYWYDARGLEMHPLERPRSVTLPAH
                     SAVTLYGSANFLGAHKVRLYLYL"
     gene            complement(6650..7009)
                     /gene="hinT"
                     /locus_tag="ACHKBL_000368"
     CDS             complement(6650..7009)
                     /gene="hinT"
                     /locus_tag="ACHKBL_000368"
                     /EC_number="3.9.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphoramidase"
                     /protein_id="HFO5817293.1"
                     /translation="MAEETIFSKIIRREIPSDIVYQDDLVTAFRDISPQAPTHILIIP
                     NILIPTVNDVSAEHEQALGRMITVAAKIAEQEGIAEDGYRLIMNTNRHGGQEVYHIHM
                     HLLGGRPLGPMLAHKGL"
     gene            7343..9532
                     /gene="fhuE"
                     /locus_tag="ACHKBL_000369"
     CDS             7343..9532
                     /gene="fhuE"
                     /locus_tag="ACHKBL_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0038023 - signaling receptor activity
                     [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric-rhodotorulic acid/ferric-coprogen
                     receptor FhuE"
                     /protein_id="HFO5817294.1"
                     /translation="MLSTQFNRDNQYQTTSKPSLLAACIALALLPSAAFAAPATEETV
                     IVEGSATTPVDDENDYSVTSTSAGTKMQMTQRDIPQSVTIVSQQRMEDQQLQTLGEVM
                     ENTLGISKSQADSDRALYYSRGFQIDNYMVDGIPTYFESRWNLGDALSDMALFERVEV
                     VRGATGLMTGTGNPSAAINMVRKHATSREFKGDVSAEYGSWNKERYVADLQSPLTEDG
                     KIRARIVGGYQNNDSWLDRYNSEKTFFSGIVDADLGDLTTLSAGYEYQRIDVNSPTWG
                     GLPRWNTDGSSNSYDRARSTAPDWAYNDKEINKVFMTLKQRFADTWQATLNATHSEVE
                     FDSKMMYVDAYVNKADGMLVGPYSNYGPGFDYVGGTGWNSGKRKVDALDLFADGSYEL
                     FGRQHNLMFGGSYSKQNNRYFSSWAYIFPDEIGSFYNFNGNFPQTDWSPQSLAQDDTT
                     HMKSLYAATRVTLADPLHLILGARYTNWRVDTLTYSMEKNHTTPYAGLVFDINDNWST
                     YASYTSIFQPQNDRDSSGKYLAPITGNNYELGLKSDWMNSRLTTTLAIFRIEQDNVAQ
                     STGTPIPGSNGETAYKAVDGTVSKGVEFELNGAITDNWQLTFGATRYIAEDNEGNAVN
                     PNLPRTTVKMFTSYRLPVMPELTVGGGVNWQNRVYTDTVTPYGTFRAEQGSYALVDLF
                     TRYQVTKNFSLQGNVNNLFDKTYDTNVEGSIVYGAPRNFSITGTYQF"
     gene            complement(9592..11025)
                     /gene="ptsG"
                     /locus_tag="ACHKBL_000370"
     CDS             complement(9592..11025)
                     /gene="ptsG"
                     /locus_tag="ACHKBL_000370"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005355 - glucose transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:1904659 - glucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HFO5817295.1"
                     /translation="MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVV
                     SHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLH
                     LPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLA
                     AIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPF
                     QMQIGEYTNAAGQVFHGDIPRYMAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPEN
                     RAKVGGIMISAALTSFLTGITEPIEFSFMFVAPILYIIHAILAGLAFPICILLGMRDG
                     TSFSHGLIDFIVLSGNSSKLWLFPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATED
                     AKATGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVV
                     AGSGVQAIFGTKSDNLKTEMDEYIRNH"
     gene            complement(11320..12117)
                     /gene="ycfH"
                     /locus_tag="ACHKBL_000371"
     CDS             complement(11320..12117)
                     /gene="ycfH"
                     /locus_tag="ACHKBL_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFO5817296.1"
                     /translation="MFLVDSHCHLDGLDYESLHKDVDDVLAKAAARDVKFCLAVATTL
                     PGYLHMRDLVGERDNVVFSCGVHPLNQNDPYDVEDLRRLAAEEGVVALGETGLDYYYT
                     PETKVRQQESFIHHIQIGRELNKPVIVHTRDARADTLAILREEKVTDCGGVLHCFTED
                     RETAGKLLDLGFYISFSGIVTFRNAEQLRDAARYVPQDRLLVETDSPYLAPVPHRGKE
                     NQPAMVRDVAEYMAVLKGVAVEELAQVTTDNFARLFHIDASRLQSIR"
     gene            complement(12128..13132)
                     /gene="holB"
                     /locus_tag="ACHKBL_000372"
     CDS             complement(12128..13132)
                     /gene="holB"
                     /locus_tag="ACHKBL_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HFO5817297.1"
                     /translation="MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYAL
                     SRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYYTLAPEKGKNTLGIDAVREVTEKLNE
                     HARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCR
                     LHYLAPPPEQYAVTWLSREVTMSQDALLAALRLSAGSPGAALALFHGDNWQARETLCQ
                     ALAYSVPSGDWYSLLAALNHEQAPARLHWLATLLMDALKRHHGAAQVTNVDVPGLVVE
                     LANHLSPSRLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL
                     "
     gene            complement(13129..13770)
                     /gene="tmk"
                     /locus_tag="ACHKBL_000373"
     CDS             complement(13129..13770)
                     /gene="tmk"
                     /locus_tag="ACHKBL_000373"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFO5817298.1"
                     /translation="MRSKYIVIEGLEGAGKTTARNVVVETLEQLGIRDMVFTREPGGT
                     QLAEKLRSLVLDIKSVGDEVITDKAEVLMFYAARVQLVETVIKPALANGTWVIGDRHD
                     LSTQAYQGGGRGIDQHMLATLRDAVLGDFRPDLTLYLDVTPEVGLKRARARGELDRIE
                     QESFDFFNRTRARYLELAAQDKSIHTIDATLPLEAVMDAIRTTVTNWVKELDA"
     gene            complement(13760..14782)
                     /gene="yceG"
                     /locus_tag="ACHKBL_000374"
     CDS             complement(13760..14782)
                     /gene="yceG"
                     /locus_tag="ACHKBL_000374"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YceG"
                     /protein_id="HFO5817299.1"
                     /translation="MKKVLLIILLLLVVLGIAAGVGVWKLRHLADSKLLIKEETIFTL
                     KPGTGRLALGEQLYADKIINRPRVFQWLLRIEPDLSHFKAGTYRFTPQMTVREMLKLL
                     ESGKEAQFPLRLVEGMRLSDYLKQLREAPYIKHTLSDDKYATVAQALELENPEWIEGW
                     FWPDTWMYTANTTDVALLKRAHKKMVKAVDSAWEGRADGLPYKDKNQLVTMASIIEKE
                     TAVASERDQVASVFINRLRIGMRLQTDPTVIYGMGERYNGKLSRADLETPTAYNTYTI
                     TGLPPGAIATPGADSLKAAAHPAKTPYLYFVADGKGGHTFNTNLASHNKSVQDYLKVL
                     KEKNAQ"
     gene            complement(14785..15594)
                     /gene="pabC"
                     /locus_tag="ACHKBL_000375"
     CDS             complement(14785..15594)
                     /gene="pabC"
                     /locus_tag="ACHKBL_000375"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008696 - 4-amino-4-deoxychorismate lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HFO5817300.1"
                     /translation="MFLINGHKQESLAVSDRATQFGDGCFTTARVIDGKVSLLSAHIQ
                     RLQDACQRLMISCDFWPQLELEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATR
                     ILSVTVYPAHYDRLRNEGMTLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNAD
                     EALVLDSEGWVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVE
                     VQASLEEALQADEMVICNALMPVMPVRACGDVSFSSATLYEYLAPLCERPN"
     gene            complement(15714..16955)
                     /gene="fabF"
                     /locus_tag="ACHKBL_000376"
     CDS             complement(15714..16955)
                     /gene="fabF"
                     /locus_tag="ACHKBL_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HFO5817301.1"
                     /translation="MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTS
                     AYATKFAGLVKDFNCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLEITEENATRIG
                     AAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISI
                     ATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQA
                     ASRPWDKERDGFVLGDGAGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPEN
                     GAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKTIFGEAASRVLVSS
                     TKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNPDEGCDLDFVPHEARQVSGME
                     YTLCNSFGFGGTNGSLIFKKI"
     gene            complement(17043..17279)
                     /gene="acpP"
                     /locus_tag="ACHKBL_000377"
     CDS             complement(17043..17279)
                     /gene="acpP"
                     /locus_tag="ACHKBL_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFO5817302.1"
                     /translation="MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELV
                     MALEEEFDTEIPDEEAEKITTVQAAIDYINGHQA"
     gene            complement(17490..18224)
                     /gene="fabG"
                     /locus_tag="ACHKBL_000378"
     CDS             complement(17490..18224)
                     /gene="fabG"
                     /locus_tag="ACHKBL_000378"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HFO5817303.1"
                     /translation="MNFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGAQA
                     ITDYLGANGKGLMLNVTDPASIESVLEKIRAEFGEVDILVNNAGITRDNLLMRMKDEE
                     WNDIIETNLSSVFRLSKAVMRAMMKKRHGRIITIGSVVGTMGNGGQANYAAAKAGLIG
                     FSKSLAREVASRGITVNVVAPGFIETDMTRALSDDQRAGILAQVPAGRLGGAQEIANA
                     VAFLASDEAAYITGETLHVNGGMYMV"
     gene            complement(18237..19166)
                     /gene="fabD"
                     /locus_tag="ACHKBL_000379"
     CDS             complement(18237..19166)
                     /gene="fabD"
                     /locus_tag="ACHKBL_000379"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFO5817304.1"
                     /translation="MTQFAFVFPGQGSQTVGMLADMAASYPIVEETFAEASAALGYDL
                     WALTQQGPAEELNKTWQTQPALLTASVALYRVWQQQGGKAPAMMAGHSLGEYSALVCA
                     GVIDFADAVRLVEMRGKFMQEAVPEGTGAMAAIIGLDDASIAKACEEAAEGQVVSPVN
                     FNSPGQVVIAGHKEAVERAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVELAK
                     ITFNAPTVPVVNNVDVKCETNGDAIRDALVRQLYNPVQWTKSVEYMAAQGVEHLYEVG
                     PGKVLTGLTKRIVDTLTASALNEPSAMAAALEL"
     gene            complement(19182..20135)
                     /gene="fabH"
                     /locus_tag="ACHKBL_000380"
     CDS             complement(19182..20135)
                     /gene="fabH"
                     /locus_tag="ACHKBL_000380"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HFO5817305.1"
                     /translation="MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHI
                     AAPNETVSTMGFEAATRAIEMAGIEKDQIGLIVVATTSATHAFPSAACQIQSMLGIKG
                     CPAFDVAAACAGFTYALSVADQYVKSGAVKYALVVGSDVLARTCDPTDRGTIIIFGDG
                     AGAAVLAASEEPGIISTHLHADGSYGELLTLPNADRVNPENSIHLTMAGNEVFKVAVT
                     ELAHIVDETLAANNLDRSQLDWLVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTS
                     AASVPCALDEAVRDGRIKPGQLVLLEAFGGGFTWGSALVRF"
     gene            complement(20203..21273)
                     /gene="plsX"
                     /locus_tag="ACHKBL_000381"
     CDS             complement(20203..21273)
                     /gene="plsX"
                     /locus_tag="ACHKBL_000381"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HFO5817306.1"
                     /translation="MTRLTLALDVMGGDFGPSVTVPAALQALNSNSQLTLLLVGNPDA
                     ITPLLAKADFEQRSRLQIIPAQSVIASDARPSQAIRASRGSSMRVALELVKEGRAQAC
                     VSAGNTGALMGLAKLLLKPLEGIERPALVTVLPHQQKGKTVVLDLGANVDCDSTMLVQ
                     FAIMGSVLAEEVVEIPNPRVALLNIGEEEVKGLDSIRDASAVLKTIPSINYIGYLEAN
                     ELLTGKTDVLVCDGFTGNVTLKTMEGVVRMFLSLLKSQGEGKKRSWWLLLLKRWLQKS
                     LTRRFSHLNPDQYNGACLLGLRGTVIKSHGAANQRAFAVAIEQAVQAVQRQVPQRIAA
                     RLESVYPAGFELLDGGKSGTLR"
     gene            complement(21354..21527)
                     /gene="rpmF"
                     /locus_tag="ACHKBL_000382"
     CDS             complement(21354..21527)
                     /gene="rpmF"
                     /locus_tag="ACHKBL_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFO5817307.1"
                     /translation="MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHIT
                     ADGYYRGRKVIAK"
     gene            complement(21579..22100)
                     /gene="yceD"
                     /locus_tag="ACHKBL_000383"
     CDS             complement(21579..22100)
                     /gene="yceD"
                     /locus_tag="ACHKBL_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HFO5817308.1"
                     /translation="MQKVKLPLTLDPVRTAQKRLDYQGIYTPDQVERVAESVVSVDSD
                     VECSMSFAIDNQRLAVLNGDAKVTVTLECQRCGKPFTHQVYTTYCFSPVRSDEQAEAL
                     PEAYEPIEVNEFGEIDLLAMVEDEIILALPVVPVHDSEHCEVSEADMVFGELPEEAQK
                     PNPFAVLASLKRK"
     gene            22299..22883
                     /gene="yceF"
                     /locus_tag="ACHKBL_000384"
     CDS             22299..22883
                     /gene="yceF"
                     /locus_tag="ACHKBL_000384"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-methyl-GTP pyrophosphatase"
                     /protein_id="HFO5817309.1"
                     /translation="MPKLILASTSPWRRALLEKLQISFECAAPEVDETPRSDESPRQL
                     VLRLAQEKAQSLASRYPDHLIIGSDQVCVLDGEITGKPLTEENARLQLRKASGNIVTF
                     YTGLALFNSANGHLQTEVEPFDVHFRHLSEAEIDNYVRKEHPLHCAGSFKSEGFGITL
                     FERLEGRDPNTLVGLPLIALCQMLRREGKNPLMG"
     gene            complement(22996..23955)
                     /gene="rluC"
                     /locus_tag="ACHKBL_000385"
     CDS             complement(22996..23955)
                     /gene="rluC"
                     /locus_tag="ACHKBL_000385"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HFO5817310.1"
                     /translation="MKTETPSVKIVAINADEAGQRIDNFLRTQLKGVPKSMIYRILRK
                     GEVRVNKKRIKPEYKLEAGDEVRIPPVRVAEREEEAVSPHLQKVAALADVILYEDDHI
                     LVLNKPSGTAVHGGSGLSFGVIEGLRALRPEARFLELVHRLDRDTSGVLLVAKKRSAL
                     RSLHEQLREKGMQKDYLALVRGQWQSHVKSVQAPLLKNILQSGERIVRVSQEGKPSET
                     RFKVEERYAFATLVRCSPVTGRTHQIRVHTQYAGHPIAFDDRYGDREFDRQLTEAGTG
                     LNRLFLHAAALKFTHPGTGEVMRIEAPMDEGLKRCLQKLRNAR"
     gene            24528..27713
                     /gene="rne"
                     /locus_tag="ACHKBL_000386"
     CDS             24528..27713
                     /gene="rne"
                     /locus_tag="ACHKBL_000386"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008995 - ribonuclease E activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HFO5817311.1"
                     /translation="MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKG
                     KITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPANYSAHGRPNIKDVLREGQEVIV
                     QIDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLEL
                     PEGMGLIVRTAGVGKSAEALQWDLSFRLKHWEAIKKAAESRPAPFLIHQESNVIVRAF
                     RDYLRQDIGEILIDNPKVLELARQHIAALGRPDFSSKIKLYTGEIPLFSHYQIESQIE
                     SAFQREVRLPSGGSIVIDSTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQL
                     RLRDLGGLIVIDFIDMTPVRHQRAVENRLREAVRQDRARIQISHISRFGLLEMSRQRL
                     SPSLGESSHHVCPRCSGTGTVRDNESLSLSILRLIEEEALKENTQEVHAIVPVPIASY
                     LLNEKRSAVNAIETRQDGVRCVIVPNDQMETPHYHVLRVRKGEETPTLSYMLPKLHEE
                     AMALPSEEEFAERKRPEQPALATFAMPDVPPAPTPAEPAAPVVAPAPKAATATPASPA
                     QPGLLSRFFGALKALFSGGEETKPAEQPAPKAEAKPERQQDRRKPRQNNRRDRNERRD
                     TRSERAEGNDNREENRRNRRQAQQQTAETRESRQQAEVTEKARTTDEQQAPRRERSRR
                     RNDDKRQAQQEAKALNVEEQSVQETEQEERVRPVQPRRKQRQLNQKVRYEQSVAEEAV
                     VAPVVEETVAAEPIVQEAPAPRTELVKVPLPVVAQTAPEQQEENNADNRDNGGMPRRS
                     RRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACASPELASGKVWIRYPIVRPQDVQV
                     EEQREQEEVQVQPMVTEVPVAAAVEPVVSAPVVEEVAEVVEAPVQVAEPQPEVAETTH
                     PEVIAAAVTEQPQVITESDVAVAQEVAEHAEPVVEPQEETADIEEVAETAEVVVAEPE
                     VVAQPAAPVVAEVAAEVETVAAVEPEITVEHNHATAPMTRAPAPEYVPEAPRHSDWQR
                     PTFAFEGKGAAGGHTATHHASAAPARPQPVE"
     gene            27847..28140
                     /locus_tag="ACHKBL_000387"
     CDS             27847..28140
                     /locus_tag="ACHKBL_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HFO5817312.1"
                     /translation="MPAANNIPARRESLNLRIKPAERDLIDRAAKARGKNRTEFVLEA
                     ARAAAEEALIEQRIFRIDSDDYQLFLNRLDQPPAPNAALRKTMQTPAPWEQKK"
     gene            28137..28631
                     /locus_tag="ACHKBL_000388"
     CDS             28137..28631
                     /locus_tag="ACHKBL_000388"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO5817313.1"
                     /translation="MIQAPEPLMARHQFTSFCSGVETMDNWLKQRALKNQLAGASRTF
                     VSCDTYSNVLAYYSLASSAVETYVATGRFRRNMPEPIPVVVLGRLAIDKSLQGQGIDR
                     AMVRDAGLRVLQAAEVIGIRGMLVHALSDQAREFYLRVGFEPSPVDSMILMATLADLQ
                     ECLK"
     gene            complement(28726..29679)
                     /gene="flgL"
                     /locus_tag="ACHKBL_000389"
     CDS             complement(28726..29679)
                     /gene="flgL"
                     /locus_tag="ACHKBL_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HFO5817314.1"
                     /translation="MRFSTQMMYQQNMRGITNSQAEWMKYGEQMSTGKRVVNPSDDPI
                     AASQAVVLSQAQAQNSQYTLARTFATQKVSLEESVLSQVTTAIQNAQEKIVYASNGTL
                     SDDDRASLATDIQGLRDQLLNLANTTDGNGRYIFAGYKTESAPFSEADGKYEGGAESI
                     KQQVDASRSMVIGHTGDKIFDSITSNAVAEPDGSASETNLFAMLDSAIAALKTPVADS
                     EADKETAAAALDKTNRGLKNSLNNVLTVRAELGTQLNELESLDSLGSDRALGQTQQMS
                     DLVDVDWNATISSYIMQQTALQASYKAFTDMQGLSLFQLNK"
     gene            complement(29691..31334)
                     /gene="flgK"
                     /locus_tag="ACHKBL_000390"
     CDS             complement(29691..31334)
                     /gene="flgK"
                     /locus_tag="ACHKBL_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HFO5817315.1"
                     /translation="MSSLINNAMSGLNAAQAALNTASNNISSYNVAGYTRQTTIMAQA
                     NSTLGAGGWVGNGVYVSGVQREYDAFITNQLRAAQTQSSGLTARYEQMSKIDNMLSTS
                     TSSLATQMQDFFTSLQTLVSNAEDPAARQALIGKSEGLVNQFKTTDQYLRDQDKQVNI
                     AIGASVDQINNYAKQIASLNDQISRLTGVGAGASPNNLLDQRDQLVSELNQIVGVEVS
                     VQDGGTYNITMANGYSLVQGSTARQLAAVPSSADPSRTTVAYVDGTAGNIEIPEKLLN
                     TGSLGGILTFRSQDLDQTRNTLGQLALAFAEAFNTQHKAGFDANGDAGEDFFAIGKPA
                     VLQNTKNKGDVAIGATVTDASAVLATDYKISFDNNQWQVTRLASNTTFTVTPDASGKV
                     AFDGLELTFTGTPAVNDSFTLKPVSDAIVNMDVLITDEAKIAMASAEDTGDSDNRNGQ
                     ALLDLQSNSKTVGGAKSFNDAYASLVSDIGNKTATLKTSSTTQGNVVTQLSNQQQSIS
                     GVNLDEEYGNLQRFQQYYLANAQVLQTANAIFDALINIR"
     gene            complement(31400..32341)
                     /gene="flgJ"
                     /locus_tag="ACHKBL_000391"
     CDS             complement(31400..32341)
                     /gene="flgJ"
                     /locus_tag="ACHKBL_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HFO5817316.1"
                     /translation="MISDSKLLASAAWDAQSLNELKAKAGEDPAANIRPVARQVEGMF
                     VQMMLKSMRDALPKDGLFSSEHTRLYTSMYDQQIAQQMTAGKGLGLAEMMVKQMTPEQ
                     PLPEESTPAAPMKFPLETVVRYQNQTLSQLVQKAVPRNYDDSLPGDSKAFLAQLSLPA
                     QLASQQSGVPHHLILAQAALESGWGQRQIRRENGEPSYNLFGVKASGNWKGPVTEITT
                     TEYENGEAKKVKAKFRVYSSYLEALSDYVGLLTRNPRYAAVTTAASAEQGAQALQDAG
                     YATDPHYARKLTSMIQQMKSISDKVSKTYSMNIDNLF"
     gene            complement(32341..33438)
                     /gene="flgI"
                     /locus_tag="ACHKBL_000392"
     CDS             complement(32341..33438)
                     /gene="flgI"
                     /locus_tag="ACHKBL_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415598.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HFO5817317.1"
                     /translation="MIKFLSALILLLVTTAAQAERIRDLTSVQGVRQNSLIGYGLVVG
                     LDGTGDQTTQTPFTTQTLNNMLSQLGITVPTGTNMQLKNVAAVMVTASLPPFGRQGQT
                     IDVVVSSMGNAKSLRGGTLLMTPLKGVDSQVYALAQGNILVGGAGASAGGSSVQVNQL
                     NGGRITNGAVIERELPSQFGVGNTLNLQLNDEDFSMAQQIADTINRVRGYGSATALDA
                     RTIQVRVPSGNSSQVRFLADIQNMQVNVTPQDAKVVINSRTGSVVMNREVTLDSCAVA
                     QGNLSVTVNRQANVSQPDTPFGGGQTVVTPQTQIDLRQSGGSLQSVRSSASLNNVVRA
                     LNALGATPMDLMSILQSMQSAGCLRAKLEII"
     gene            complement(33450..34148)
                     /gene="flgH"
                     /locus_tag="ACHKBL_000393"
     CDS             complement(33450..34148)
                     /gene="flgH"
                     /locus_tag="ACHKBL_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HFO5817318.1"
                     /translation="MQKNAAHTYAISSLLVLSLTGCAWIPSTPLVQGATSAQPVPGPT
                     PVANGSIFQSAQPINYGYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKT
                     NFGFDTVPRYLQGLFGNARADVEASGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGN
                     LHVVGEKQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVADARIEYVGNGYINEAQN
                     MGWLQRFFLNLSPM"
     gene            complement(34201..34983)
                     /gene="flgG"
                     /locus_tag="ACHKBL_000394"
     CDS             complement(34201..34983)
                     /gene="flgG"
                     /locus_tag="ACHKBL_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HFO5817319.1"
                     /translation="MISSLWIAKTGLDAQQTNMDVIANNLANVSTNGFKRQRAVFEDL
                     LYQTIRQPGAQSSEQTTLPSGLQIGTGVRPVATERLHSQGNLSQTNNSKDVAIKGQGF
                     FQVMLPDGSSAYTRDGSFQVDQNGQLVTAGGFQVQPAITIPANALSITIGRDGVVSVT
                     QQGQAAPVQVGQLNLTTFMNDTGLESIGENLYTETQSSGAPNESTPGLNGAGLLYQGY
                     VETSNVNVAEELVNMIQVQRAYEINSKAVSTTDQMLQKLTQL"
     gene            complement(35154..35909)
                     /gene="flgF"
                     /locus_tag="ACHKBL_000395"
     CDS             complement(35154..35909)
                     /gene="flgF"
                     /locus_tag="ACHKBL_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
                     /protein_id="HFO5817320.1"
                     /translation="MDHAIYTAMGAASQTLNQQAVTASNLANASTPGFRAQLNALRAV
                     PVEGLSLPTRTLVTASTPGADMTPGKMDYTSRPLDVALQQDGWLAVQTADGSEGYTRN
                     GSIQVDPTGQLTIQGHPVIGEAGPIAVPEGAEITIAADGTISALNPGDPANTVAPVGR
                     LKLVKATGSEVQRGDDGIFRLSAETQTTRGPVLQADPTLRVMSGVLEGSNVNAVAAMS
                     DMIASARRFEMQMKVISSVDDNAGRANQLLSMS"
     gene            complement(35929..37134)
                     /gene="flgE"
                     /locus_tag="ACHKBL_000396"
     CDS             complement(35929..37134)
                     /gene="flgE"
                     /locus_tag="ACHKBL_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HFO5817321.1"
                     /translation="MAFSQAVSGLNAAATNLDVIGNNIANSATYGFKSGTASFADMFA
                     GSKVGLGVKVAGITQDFTDGTTTNTGRGLDVAISQNGFFRLVDSNGSVFYSRNGQFKL
                     DENRNLVNMQGLQLTGYPATGTPPTIQQGANPTNISIPNTLMAAKTTTTASMQINLNS
                     SDPLPSVNAFDASNADSYNKKGSVTVFDSQGNAHDMSVYFVKTGDNNWQVYTQDSSDP
                     TGTAEPAMTLVFNANGVLTSDPTANISTGAINGAEPATFSLSFLNSMQQNTGANNIVA
                     TTQNGYKPGDLVSYQINDDGTVVGNYSNEQTQLLGQIVLANFANNEGLASEGDNVWSA
                     TQSSGVALLGTAGTGNFGTLTNGALEASNVDLSKELVNMIVAQRNYQSNAQTIKTQDQ
                     ILNTLVNLR"
     gene            complement(37159..37854)
                     /gene="flgD"
                     /locus_tag="ACHKBL_000397"
     CDS             complement(37159..37854)
                     /gene="flgD"
                     /locus_tag="ACHKBL_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HFO5817322.1"
                     /translation="MSIAVTTTDPTNTGVSTTSSSSLTGSNAADLQSSFLTLLVAQLK
                     NQDPTNPMENNELTSQLAQISTVSGIEKLNTTLGSISGQIDNSQSLQASNLIGHGVMI
                     PGTTVLAGTGSEEGAVTTTTPFGVELQQAADKVTATITDKNGAVVRTIDIGELTAGVH
                     SFTWDGTLTDGTTAPNGSYNVAISASNGGTQLVAQPLQFALVQGVIRGNNGNTLDLGT
                     YGTTTLDEVRQII"
     gene            complement(37866..38270)
                     /gene="flgC"
                     /locus_tag="ACHKBL_000398"
     CDS             complement(37866..38270)
                     /gene="flgC"
                     /locus_tag="ACHKBL_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HFO5817323.1"
                     /translation="MALLNIFDIAGSALTAQSQRLNVAASNLANADSVTGPDGQPYRA
                     KQVVFQVNAAPGAATGGVKVADVIESQAPDKLVYEPGNPLADAKGYVKMPNVDVVGEM
                     VNTMSASRSYQANVEVLNTVKSMMLKTLTLGQ"
     gene            complement(38274..38690)
                     /gene="flgB"
                     /locus_tag="ACHKBL_000399"
     CDS             complement(38274..38690)
                     /gene="flgB"
                     /locus_tag="ACHKBL_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HFO5817324.1"
                     /translation="MLDKLDAALRFQQEALNLRAQRQEVLAANIANADTPGYQARDID
                     FASELKKVMQRGRDATSVVALTMTSTQHIPAQALTPPTAELQYRIPDQPSLDGNTVDM
                     DRERTQFADNSLQYQMSLSALSGQIKGMMNVLQSGN"
     gene            38846..39505
                     /gene="flgA"
                     /locus_tag="ACHKBL_000400"
     CDS             38846..39505
                     /gene="flgA"
                     /locus_tag="ACHKBL_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HFO5817325.1"
                     /translation="MLAIKRSVAIIAILFSPLSAASNLTSQLHTFFSAQLAGVSDEVR
                     VSIRTAPNLLPPCEQPLLSMSNNSRLWGNVNVLARCGNDKRYLQVNVQATGNYVVAAM
                     PIVRGGKLEAGNVKLKRGRLDTLPPRTVLDINQLVDAVSLRDLSPDQPIQLTQFRQAW
                     RVKAGQRVNVIASGDGFSANAEGQALNNAAVAQNARVRMVSGQVVSGVVDADGNILIN
                     L"
     gene            39581..39874
                     /gene="flgM"
                     /locus_tag="ACHKBL_000401"
     CDS             39581..39874
                     /gene="flgM"
                     /locus_tag="ACHKBL_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HFO5817326.1"
                     /translation="MSIDRTSPLKPVSTVQPRETTDAPVTNTRAAKTTASTSTSVTLS
                     DAQAKLMQPGSSDINLERVEALKLAIRNGELKMDTGKIADALINEAQQDLQSN"
     gene            39879..40295
                     /gene="flgN"
                     /locus_tag="ACHKBL_000402"
     CDS             39879..40295
                     /gene="flgN"
                     /locus_tag="ACHKBL_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FlgN"
                     /protein_id="HFO5817327.1"
                     /translation="MTRLAEILDQMSAVLNDLKTVMDQEQQHLSMGQINGSQLQWITE
                     QKSSLLATLDYLEQLRRKEPNAANSVDISQRWQEITVKTQQLRQMNQHNGWLLEGQIE
                     RNQQALEMLKPHQEPTLYGANGQTSTTHRGSKKISI"
     gene            complement(40335..41870)
                     /gene="murJ"
                     /locus_tag="ACHKBL_000403"
     CDS             complement(40335..41870)
                     /gene="murJ"
                     /locus_tag="ACHKBL_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HFO5817328.1"
                     /translation="MNLLKSLAAVSSMTMFSRVLGFARDAIVARIFGAGMATDAFFVA
                     FKLPNLLRRIFAEGAFSQAFVPILAEYKSKQGEDATRVFVSYVSGLLTLALAVVTVAG
                     MLAAPWVIMVTAPGFADTADKFALTSQLLKITFPYILLISLASLVGAILNTWNRFSIP
                     AFAPTLLNISMIGFALFAAPYFNPPVLALAWAVTVGGILQLVYQLPHLKKIGMLVLPR
                     INFHDAGAMRVVKQMGPAILGVSVSQISLIINTIFASFLASGSVSWMYYADRLMEFPS
                     GVLGVALGTILLPSLSKSFASGNHDEYNRLMDWGLRLCFLLALPSAVALGILSGPLTV
                     SLFQYGKFTAFDALMTQRALIAYSVGLIGLIVVKVLAPGFYSRQDIKTPVKIAIVTLI
                     LTQLMNLAFIGPLKHAGLSLSIGLAACLNASLLYWQLRKQKIFTPQPGWMAFLLRLVV
                     AVLVMSGVLLGMLHIMPEWSLGTMPWRLLRLMAVVLAGIAAYFAALAVLGFKVKEFAR
                     RTV"
     gene            complement(41980..42903)
                     /gene="yceM"
                     /locus_tag="ACHKBL_000404"
     CDS             complement(41980..42903)
                     /gene="yceM"
                     /locus_tag="ACHKBL_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
                     /protein_id="HFO5817329.1"
                     /translation="MKKLRIGVVGLGGIAQKAWLPVLAAASDWMLQGAWSPTRAKALP
                     ICESWRIPYADSLSSLAASCDAVFVHSSTASHFDVVSTLLNTGVHVCVDKPLAENLRD
                     AERLVELAARKKLTLMVGFNRRFAPLYGELKTQLATAASLRMDKHRSNSVGPHDLYFT
                     LLDDYLHVVDTALWLSGGKASLDGGTLLTNDAGEMLFAEHHFSAGPLQITTCMHRRAG
                     SQRETVQAVTDGALIDITDMREWREERGQGVVHKPIPGWQSTLEQRGFVGCARHFIEC
                     VQNQTVPQTAGEQAVRAQRIVDKIWRDAMSE"
     gene            complement(42905..43552)
                     /gene="yceH"
                     /locus_tag="ACHKBL_000405"
     CDS             complement(42905..43552)
                     /gene="yceH"
                     /locus_tag="ACHKBL_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HFO5817330.1"
                     /translation="MKYQLTALEARVIGCLLEKQVTTPEQYPLSVNGVVTACNQKTNR
                     EPVMNLSESEVQEQLDNLVKRHYLRTVSGFGNRVTKYEQRFCNSEFGDLKLSAAEVAL
                     ITTLLLRGAQTPGELRSRAARMYEFSDMAEVESTLEQLANREDGPFVVRLAREPGKRE
                     SRYMHLFSGEVEDQPAVMDMSNAVDGDLQARVEALEIEVAELKQRLDSLLAHLGD"
     gene            complement(43563..44147)
                     /gene="rimJ"
                     /locus_tag="ACHKBL_000406"
     CDS             complement(43563..44147)
                     /gene="rimJ"
                     /locus_tag="ACHKBL_000406"
                     /EC_number="2.3.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S5-alanine
                     N-acetyltransferase"
                     /protein_id="HFO5817331.1"
                     /translation="MFGYRSNVPKVRLTTDRLVVRLVHDRDAWRLADYYAENRHFLKP
                     WEPVRDESHCYPSGWQARLGMINEFHKQGSAFYFGLFDPDEKEIIGVANFSNVVRGSF
                     HACYLGYSIGQKWQGKGLMFEALTAAIRYMQRTQHIHRIMANYMPHNKRSGDLLARLG
                     FEKEGYAKDYLLIDGQWRDHVLTALTTPDWTPGR"
     gene            44383..45591
                     /gene="mdtH"
                     /locus_tag="ACHKBL_000407"
     CDS             44383..45591
                     /gene="mdtH"
                     /locus_tag="ACHKBL_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtH"
                     /protein_id="HFO5817332.1"
                     /translation="MSRVSQARNLGKYFLLIDNMLVVLGFFVVFPLISIRFVDQMGWA
                     AVMVGIALGLRQFIQQGLGIFGGAIADRFGAKPMIVTGMLMRAAGFATMGIAHEPWLL
                     WFSCLLSGLGGTLFDPPRSALVVKLIRPQQRGRFFSLLMMQDSAGAVIGALLGSWLLQ
                     YDFRLVCATGAVLFVLCAAFNAWLLPAWKLSTVRTPVREGMTRVMRDKRFVTYVLTLA
                     GYYMLAVQVMLMLPIMVNDVAGAPSAVKWMYAIEACLSLTLLYPIARWSEKHFRLEHR
                     LMAGLLIMSLSMMPVGMVSGLQQLFTLICLFYIGSIIAEPARETLSASLADARARGSY
                     MGFSRLGLAIGGAIGYIGGGWLFDLGKSAHQPELPWMMLGIIGIFTFLALGWQFSQKR
                     AARRLLERDA"
     gene            45655..46302
                     /gene="grxB"
                     /locus_tag="ACHKBL_000408"
     CDS             45655..46302
                     /gene="grxB"
                     /locus_tag="ACHKBL_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 2"
                     /protein_id="HFO5817333.1"
                     /translation="MKLYIYDHCPYCLKARMIFGLKNIPIELHVLLNDDAETPTRMVG
                     QKQVPILQKDDSRYMPESMDIVHYVDKLDGKPLLTGKRSPAIEEWLRKVNGYANKLLL
                     PRFAKSAFDEFSTPAARKYFVDKKEASAGNFADLLAHSDGLIKNISDDLRALDKLIVK
                     PNAVNGELSEDDIQLFPLLRNLTLVAGINWPSRVADYRDNMAKQTQINLLSSMAI"
     gene            46436..46996
                     /gene="yceB"
                     /locus_tag="ACHKBL_000409"
     CDS             46436..46996
                     /gene="yceB"
                     /locus_tag="ACHKBL_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFO5817334.1"
                     /translation="MNKFLFAAALIVSGLLVGCNQLTQYTITEQEINQSLAKHNNFSK
                     DIGLPGVADAHIVLTNLTSQIGREEPNKVTLTGDANLDMNSLFGSQKATMKLKLKALP
                     VFDKEKGAIFLKEMEVVDATVQPEKMQTVMQTLLPYLNQALRNYFNQQPAYVLREDGS
                     QGEAMAKKLAKGIEVKPGEIVIPFTD"
     gene            47102..48148
                     /gene="pyrC"
                     /locus_tag="ACHKBL_000410"
     CDS             47102..48148
                     /gene="pyrC"
                     /locus_tag="ACHKBL_000410"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_process: GO:0019856 - pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HFO5817335.1"
                     /translation="MTAPSQVLKIRRPDDWHLHLRDGDMLKTVVPYTSEIYGRAIVMP
                     NLAPPVTTVEAAVAYRQRILDAVPAGHDFTPLMTCYLTDSLDPNELERGFNEGVFTAA
                     KLYPANATTNSSHGVTSVDAIMPVLERMEKIGMPLLVHGEVTHADIDIFDREARFIES
                     VMEPLRQRLTALKVVFEHITTKDAADYVRDGNERLAATITPQHLMFNRNHMLVGGVRP
                     HLYCLPILKRNIHQQALRELVASGFNRVFLGTDSAPHARHRKESSCGCAGCFNAPTAL
                     GSYATVFEEMNALQHFEAFCSVNGPQFYGLPVNDTFIELVRKEQQVAESIALTDDTLV
                     PFLAGETVRWSVKQ"
     gene            48222..48467
                     /gene="dinI"
                     /locus_tag="ACHKBL_000411"
     CDS             48222..48467
                     /gene="dinI"
                     /locus_tag="ACHKBL_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein I"
                     /protein_id="HFO5817336.1"
                     /translation="MRIEVTIAKTSPLPAGAIDALAGELSRRIQYAFPDNEGHVSVRY
                     AAANNLSVIGATKEDKQRISEILQETWESADDWFVSE"
     gene            48757..49011
                     /gene="bssS"
                     /locus_tag="ACHKBL_000412"
     CDS             48757..49011
                     /gene="bssS"
                     /locus_tag="ACHKBL_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309465.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssS"
                     /protein_id="HFO5817337.1"
                     /translation="MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSEQEGT
                     EVGQTLWLTTDVARQFISILEAGIAKIESGDFPVNEYRRH"
     gene            49126..50244
                     /gene="solA"
                     /locus_tag="ACHKBL_000413"
     CDS             49126..50244
                     /gene="solA"
                     /locus_tag="ACHKBL_000413"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050131 - N-methyl-L-amino-acid oxidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HFO5817338.1"
                     /translation="MKYDLIIIGSGSVGAAAGYYATRAGLNVLMTDAHMPPHQHGSHH
                     GDTRLIRHAYGEGEKYVPLVLRAQTLWDELSRHNEEDPIFVRSGVINLGPADSTFLAN
                     VAHSAEQWQLNVEKLDAQGIMARWPEIRVPDNYIGLFETDSGFLRSELAIKTWIQLAK
                     EAGCAQLFNCPVTAIRHDDDGVTIETADGEYQAKKAIVCAGTWVKDLLPELPVQPVRK
                     VFAWYQADGRYSVKNKFPAFTGELPNGDQYYGFPAENDALKIGKHNGGQVIHSADERV
                     PFAEVVSDGSEAFPFLRNVLPGIGCCLYGAACTYDNSPDEDFIIDTLPGHDNTLLITG
                     LSGHGFKFASVLGEIAADFAQDKKSDFDLTPFRLSRFQ"
     gene            50292..50405
                     /locus_tag="ACHKBL_000414"
     CDS             50292..50405
                     /locus_tag="ACHKBL_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceO family protein"
                     /protein_id="HFO5817339.1"
                     /translation="MRRLLHYLINNIREHLMLYLFLWGLLAIMDLIYVYYF"
BASE COUNT        12252 a        13597 c        12543 g        12151 t
ORIGIN      
        1 tttataataa cctttcattc acagtcacca ggtacaacgg catcatggca actgactcaa
       61 cacaatgtgt aaaaaaaagc cgcggccgcc caaaagtgtt cgacagggat gccgcgcttg
      121 ataaggccat gaaattgttc tggcaacacg gttatgaagc gacttctctt gcggacctcg
      181 tcgaagcgac cggagccaaa gcgcccacgc tatacgcgga atttaccaac aaagaggggt
      241 tattccgcgc cgtactcgac cgctatatcg atcgttttgc cgctaagcat gaagcacagc
      301 tgttttgtga agagaaaagc gtggagtctg cgctggctga ctattttgct gccatcgcca
      361 actgctttac cagcaaagac accccggcag gctgcttcat gatcaacaac tgcaccaccc
      421 tctccccaga ttcaggagat atcgccaata cgctgaaatc acgccatgcg atgcaagagc
      481 gcactttgca gcagttttta tgtcaacgac aagcgcgcgg ggaaatcccg acccactgtg
      541 acgtgacgca tctggcagaa ttccttaatt gtattattca ggggatgtcg atcagcgcac
      601 gcgaaggtgc atcgctggaa aaactgatgc agattgcccg aacgactttg cgtttatggc
      661 ccgaactggt gaaataaggc gcaaaaaaag cccctcagcc tgagcgaatg aggggccaaa
      721 ttgcagagta cagccttgtt atactttgtt attcaaaatc agttgcccgt tgctatctag
      781 cgggatctgc gtacccggat cgcggtccat gcggattttg ccctgctgat cgccaatctt
      841 ataggtcaca tcataaccga gcattttttc tgacttgtca tacaccgttt tacaacgctg
      901 ttgcgtagtc gtgtaagtat cgctttcctg gagagagccc tggatctggt taccggcata
      961 tccaccaccc agcgccccca caacagtagc gacatcttta ccgcgaccac caccaaactg
     1021 atgccctatc acgccgccag caacagcgcc gagcaccgac ccggtaatgc gattttcatc
     1081 ctgcaccggt cgacgatggg tcactgtgac gttacgacac tcctgacgcg gtgttttaac
     1141 cgtttccttg attggggttg cagaaacaac ctgagcgtat tgcgggccac gttcaaacac
     1201 gttcagactg gccactgccg ctacgcccaa cgcagctgcg acaccaatcc cgatacccgc
     1261 caacattgat ttattcacgg gaatacctcc ttctttgcag ttatgccaat catgcaagcg
     1321 aaggagaaaa gcagctatca gacagaagat gaaaagcgcg ggaatggctg gaaatactta
     1381 gggattcgga ggagtctgaa ggaatgcctg atgcgacact taacgcgtct tatcaggcct
     1441 accgctcgta ccatttgtcc agggcggata aggcgtttat gccccatccg ccagtacaag
     1501 ccgattaatg caacttcaaa cgtggacgga taacgcggtt aatactcccc accagcatca
     1561 ttaatccagt tttaaagtaa ccatgcagcg caatctgatg cattcggtat agcgagatat
     1621 atacaaagcg cgcaattcgt ccttcaatca tcattgagcc gcgtgtcagg ttacccatca
     1681 ggctaccgac ggtggaaaag ttcgacagcg ataccagcga accgtgatct ttatactgat
     1741 aatttttcag cggcttaccg ttcatctgcg ccagaatgtt gttcattgcg caggttgcca
     1801 tctgatgtgc ggcctgagcg cgcggcggaa caaagccccc ttccggacgc gggcatgacg
     1861 cgcagtcgcc aatagcgtat atgtctggat cgcgagtggt ttgcagcgtc ggttccacca
     1921 ccagctggtt gatacggttg gtttcaagac caccgatatc tttcaggaag tctggcgctt
     1981 tgatcccggc tgcccacacc atcagatcag cctcaatata ttcgccatct ttagtgtgca
     2041 ggccgccttc atcagcactg gtgaccatgg tttgcgtcag cacgcgaacg ccaagtttcg
     2101 ttaactcact atgggccgca gcagagatac gtggcggcaa tgcaggcaaa atacgttcgc
     2161 ccgcttctac cagcgttacg ttcagggctt cgttggtcag acctttataa ccgtaactgt
     2221 gcagttgctt gaccgcgttg tgcaattcag cggagagttc tacacccgtc gcgccgccgc
     2281 cgacaatcgc aatgtttact ttgccattcg cgcccaggtt ggcggagtat ttcaggaaca
     2341 gattcagcat ctcctggtgg aagcgacgcg cctggtgcgg gttatcgagg aaaatgcagt
     2401 tctctttgac acctggcgta ttgaaatcgt tggaggtgct acccagcgcc attaccaggg
     2461 tgtcataggc gattttacgt tccggaacca gcaattcacc tttttcgtcg cgcagttctg
     2521 caatagtgat tgttttcgct tcacgatcaa tatcaattac ggaacccagc tggaactgga
     2581 aaccatgatt gcgcgcatgg gccagatagc tcaacgcatc gacgccttca tcaagcgagc
     2641 cagtcgccac ttcgtgcagc agcggtttcc acaggtggct gtggttacga tcgaccagcg
     2701 taattttggc ttttttcttg cgtcccagct tatgccccag ctgtgttgcc atttccagcc
     2761 caccagcacc gccgccgaca atcacaattt ttttcaatgg cgtagtcaac gtgaccccct
     2821 taaatttatt aaccaattgt taattaatgg ttattaacat agctcttaat taacaacagg
     2881 ttacagcaat gaaaatgttc ttcactattg agaataacat gaatggtgca ttggtcatac
     2941 caaaattgat gttaatcaag ttttgttgct gaaaagatag gcgactttac cgggcgagat
     3001 caaaaaacag agtttattgt ttattatttg agcgaatttg tgagagtgat atagagtgag
     3061 gggtgtatgg agtgcgttcg gtaggcctga taagaggcgc atcaggcatt gatgctcgac
     3121 tgtcggatgc ggcgtggacg ccttatccga cttacagaat tcggagcgca tcaggtaccg
     3181 gcaaggttac ctgatgcgac gagggcattt acccgagggt tttgaacgcc ttaatgcgct
     3241 gcaaatgcgg ggagatattc ttgaatttgt gcgtctgctc ttcgtcccag acaatctcgt
     3301 aataatgatg cagctcttca gatgtccgct ggctgttaag agcttcatca ttacgcgaca
     3361 aaatcaccaa acaacgatcg cgattctttt cgcggaagtt ggtcacacac ttagtggcaa
     3421 tatcggcata ctcttccggg cgatcaatct tgccttccat gttctcataa gggaacaaat
     3481 tagggttgaa gatcacctgg cggatatcgc aaagaaaacc aatccgttcc gcccagtatc
     3541 cgcccagacc aacgccgcaa atcagcggac gctcgtcgac gttcagttgc aacattttgt
     3601 ccacttcttt gagcagatgc tgcatatcat gtttcggatg ccgcgtactg tagcttatca
     3661 agcgtacatc cgggtcaata aactgcaatt gtaagacttt ctcgtggtta cccggactgt
     3721 tagagtcaaa accgtgtaaa tagataatca tcgcttcctc acataagcca gggttagtga
     3781 cctgctttct cttcctgcca gcgttcatgt aactgattca gacgggcgct gatcgctttc
     3841 cagcgagccg agtccatcag ttcctgtcgc gaaaatgaac ctttatgata caaacgtgta
     3901 acacgttctg ccttgatcgg tgacagatta tctaacacgc tgacggcccc tttacgatta
     3961 ttgcagacca ggatcatatc gcaacccgcg tccagtgatg cctgcccgcg ttcggcataa
     4021 ctgcccataa tcgcggctcc ttccatcgat aaatcgtcag agaaaatcac gccatcaaaa
     4081 cccagttcct gacgcaaaac ggttttcagc caatagggag aaccgctcgc cggacgcgga
     4141 tcgacatcac tgtagatcac atgcgcaggc ataatggcgt cgagtttatt ttcgcggatt
     4201 aaggaactga agaccgacat atctttagcg cgaatctccg cttgcggacg tgggtcgcac
     4261 ggtgtttctt tgtgtgagtc tgccgttact gcaccgtgtc ctgggaagtg tttcccggtc
     4321 gttttcattc cggcttcatg cataccatca ataaaccggc tggcaattgc cagggctttt
     4381 tcgggatcgg aatgataaga acgctcgcca atcgccgcgc tgatatgccc gacgtccagc
     4441 actggcgcaa agctgatatc aatatccata gcaatcattt cgctggccat caaccaacct
     4501 gcctctcgcg ccagttttcc gccttcttcc attcccaaca gcgcagcaaa tgattgtgcc
     4561 gctggcaagc gggtaaaacc ttcacgaaaa cgctgcacgc gtccaccttc ctgatcaacc
     4621 gccaccacca gatgattgcg cgaagctgcg cggatctggc gcaccagttc acgtaactgg
     4681 gcaggatcat gatagttacg cgtaaagaga atcagccctc ccaccagcgg atgcgccagt
     4741 atttcacgct cttccgcgtc cagttcgtaa ccttcgacat ccaacattac tggacccaca
     4801 ctgctctcct tattgtttta ttaatagctg ccgccaggtg tcatcggcca gcctgataaa
     4861 ttgttgatcg ccggtttgtc gccagcggta ctcaaaccac cctgctttga gcatcagcag
     4921 ccagggaaac catcgcctga cctgacgcca taattgcgcc ggataaatct tcgcgcgagt
     4981 ggcatagtca ttgaccaatt gccggtgctg gtcagtattt tccacccaca ccgccgccag
     5041 ttccagcgcg atatcaccat ctccggcata ctcccagtcg atgagtttta accctgacgc
     5101 gctatgcact aaatttccgg cgtggacatc catatgcaat ggacttaagc gtaaaggccg
     5161 tggttcccgc gctttgcgca gacgttttaa ccttcgcagc caacccactg tccgccgcgc
     5221 cggatcgctt tgctgccagt acagttccag taacggcaac agcgttattc gccagccaaa
     5281 acgcggttgt tgatgtagat aatacagcaa gcctgccagt tcgttggtat ccggcaaata
     5341 cgtttttacc tcgccgggca gatagtcgac caccatccag tcacggagat ataaatgcgg
     5401 cttcggtgca atgcatgcgg gtagttgtga taaagcccga tactggcgca agaacgcgaa
     5461 ccgcggcgca tcaggatcgt gcggctgacg cacaacaaaa cgctggccct gatgttcaat
     5521 gagaaaactc ccgccactaa gcccactatt aaacgtcgtg acgggatgaa actgcgggaa
     5581 aaagcgcgac agcaattcgt cgcgcgtgat gggattattg ctgcgaaacg gcacctttac
     5641 ctgaccagat aatttcgccc gtctgcacca gcatcagctg catttgaagg gtcggagcgt
     5701 taacattgcc agaggcgctg gagtacagca cgtaatgagc gccgacattg cgggcaatgc
     5761 ctatggcttt actacgggtg cctaaactgt cctgcggcga caaacctaac tgttgctttg
     5821 ccatcgacag ctgctgggcg gaaaccaggg taaatttacc gttattagcc agcgcatttc
     5881 gcagcgtttc ggtcgcttct gcggcattca gcgaaccgtt agtacggttg ttaacgctat
     5941 caaccagcag gacgctaccc gcagtcacgc cgtcagcccc aagcatctta ctgaccatcg
     6001 gctgcattgc gccattccag tcataatggc gaatatgcgg cgcaggcggt gcagtttgat
     6061 cttcgtgctc aattgggcct ggctgctgcg gtatcgtcgg caccgatggc actgtgggga
     6121 caggctgttg cggctcggct ggttgttccg gcgctggttt cacttcttct accggcgcag
     6181 gttcacgttg ccccacacac ccggcgagaa acatcgccag cgcggtaatc aaggcgtagc
     6241 gactcatttt ggtcatcaag attcacccct tacaaatata gataaagtct gactttgtgc
     6301 gcccccagaa aattggcgct gccgtacagc gttaccgccg aatgtgcggg aagggtaacg
     6361 ctgcgtggcc tttccagagg atgcatctcc agccctctgg cgtcatacca gtaaaaacga
     6421 taatgaacgg taacgggttc ttgcctttcg ttataaagcg ttgaggatgc tgaaggttga
     6481 atatcagacg tcgaaaggac gggcttttct gcactgatgc ccgcagccaa taaagatgac
     6541 tccatcacca gcgattgctc atcattcacc ggaatttccg gatgtgaacg acagcccacc
     6601 agcactaaca acgccagaga caccagccca aagcatcctt ttctcattgt tacagacctt
     6661 tatgcgccag cattggtccc agtggacggc cacccaacaa atgcatatgg atgtggtaaa
     6721 cctcttgtcc gccatggcgg ttggtgttca tgatcaggcg atagccatct tcggcaatac
     6781 cttcttgctc agcaattttt gccgctacgg tgatcatgcg tcccagcgcc tgctcatgct
     6841 cagctgacac gtcgttcaca gtcggaatga ggatattcgg aatgatcaga atatgcgttg
     6901 gcgcttgcgg cgaaatatcg cgaaacgccg ttaccagatc atcctggtag acgatatcgg
     6961 aggggatctc acgacgaata attttgctga atatagtttc ttctgccacg acattttcct
     7021 ttttcaaata gccatgcgtt gcgccatgct acgccgcgcc acactgcgag tatagagtat
     7081 ggtcgagtta aacctgctct ttcaacctta actcacgatt tcttaagcaa aaaatcgttt
     7141 tgctgccgta tatatcgcca ttattcccat ttctgcctgc gaatgcgctg aaaagacatt
     7201 ctctgtttgg tttacacgtc aaatatgaat gcgtatattt ctcatttgca tttacatgca
     7261 aaattattcg cacactaaat ataaccgctc atcaccctcc tccggcatga gcctgacgac
     7321 aacataaacc aagagatttc agatgctttc aacacaattt aacagggata atcaatatca
     7381 aaccacctcc aaaccgtcac tgcttgccgc ctgcatagca ctggcactct taccttccgc
     7441 cgcttttgct gcaccagcca ctgaagaaac ggtgattgtt gagggttcag ccactactcc
     7501 agttgatgac gaaaatgatt acagcgtaac gtctacctct gcgggtacca aaatgcagat
     7561 gactcaacgt gatattcctc agtcggtcac tattgttagt cagcagcgga tggaagatca
     7621 gcagttacaa acgctgggcg aagtgatgga aaacacgctg gggatcagca aaagccaagc
     7681 ggattccgat cgtgctcttt attattcccg cggattccag atcgataact atatggttga
     7741 tggtatcccc acctattttg aatcgcgctg gaatctgggc gacgcacttt ctgatatggc
     7801 actgtttgaa cgcgtagaag tagtgcgtgg cgcgacagga ctcatgaccg ggacgggtaa
     7861 tccatctgcg gcaattaata tggttcgaaa acacgcgacc agtcgtgaat ttaaaggcga
     7921 tgtctcggcg gaatacggta gctggaacaa agaacggtat gtggcggatt tacaaagccc
     7981 actcaccgaa gatggtaaaa tccgcgcacg aattgtcggc ggctaccaga ataacgactc
     8041 atggctggac cgctacaaca gtgaaaagac cttcttctcc ggcattgtcg atgctgattt
     8101 aggcgatctt acgacgcttt cagccggtta cgaatatcag cgcattgatg ttaatagccc
     8161 aacctggggc ggtttaccgc gctggaatac tgatggcagc agcaacagtt acgatcgcgc
     8221 acgcagtacc gcacctgact gggcgtataa cgataaagag atcaacaagg tctttatgac
     8281 cctcaagcag cggtttgctg atacctggca agcgacactg aatgccaccc actccgaagt
     8341 cgaatttgac agtaaaatga tgtatgtcga tgcctatgta aacaaagcgg atggtatgct
     8401 ggttgggcca tacagtaatt atggacctgg ctttgattat gtcggcggta ccggttggaa
     8461 cagcggcaaa cgtaaagttg atgcgctgga tttgtttgct gacggtagtt atgaattgtt
     8521 tggtcgtcag cacaatctaa tgtttggtgg cagttacagc aaacaaaaca atcgttactt
     8581 cagttcatgg gcctacatct tcccggatga aattggcagt ttctacaact ttaatggcaa
     8641 tttcccacaa accgactggt caccacagag cctggcgcag gacgatacca cacatatgaa
     8701 atcgttatat gctgccactc gtgtcaccct tgccgatccg ctgcatctga tcctcggcgc
     8761 acgttatacc aactggcggg ttgatacgct gacttacagc atggagaaaa accacaccac
     8821 gccttacgct ggtctggtgt ttgacatcaa tgacaactgg tcgacctacg ccagctatac
     8881 ctctattttc cagccgcaaa atgatcgtga cagttcaggc aaatatctgg ctccaatcac
     8941 cggtaacaac tacgagctgg gtctgaaatc ggactggatg aatagccgtc tgaccaccac
     9001 gttagccatc ttccgtattg agcaggataa tgtcgctcag tccaccggta cacctatccc
     9061 cggcagcaac ggcgaaaccg cctataaagc ggtggatggg acagtcagta aaggtgtgga
     9121 atttgaactc aacggcgcaa ttaccgacaa ctggcagctg acgtttggcg caacgcgcta
     9181 tattgcagag gataacgaag gaaacgccgt taatcctaat ctgccacgca ccacggttaa
     9241 aatgttcacc agctaccggt tgcctgtcat gccagagttg acggtcggcg gtggtgttaa
     9301 ctggcaaaat cgcgtgtata ccgacaccgt gacaccgtat ggcaccttcc gcgccgagca
     9361 aggtagctac gcgctggtgg atctcttcac ccgctaccag gtgacgaaaa acttctcgtt
     9421 acaggggaat gtcaataacc tgttcgacaa aacctacgat accaacgtgg aaggttctat
     9481 tgtctacggc gcaccgcgta atttcagcat taccggcacg tatcaattct gaataacacc
     9541 tgtaaaaaag gcagccatct ggctgcctta gtctccccaa cgtcttacgg attagtggtt
     9601 acggatgtac tcatccatct cggttttcag gttatcggat ttagtaccga aaatcgcctg
     9661 aacaccagaa ccagcaacca ctacgcccgc tgcgcccagt ttcttcaggc cagcctgatc
     9721 cactttagac acatcagcaa cgctgacgcg cagacgggta atacatgcgt cgaggttagt
     9781 aatgttttct ttaccaccaa atgcagcaac cagagccggt gccatttcgc tggtacctgt
     9841 cgcttttgcg tcttcagtcg cgtcttcacg acccggcgtt ttcagatcca gtgctttaat
     9901 cagcacgcgg aagatggtgt agtaaacaat ggcataaccg ataccgacga tcgggaacag
     9961 ccacagtttg ctgctgttac cagacagaac gatgaagtcg atcagaccgt gcgagaacga
    10021 cgtaccgtca cgcatcccca gaagaataca gattgggaat gccaggcctg ccagaatcgc
    10081 gtggatgatg tacaggatcg gcgcaacgaa catgaaggag aactcgatcg gctcggtgat
    10141 accggtcagg aacgaggtca gcgccgcgga gatcataata ccgcccactt tcgcgcggtt
    10201 ttctggttta gcagagtgcc agatagcaat tgcggcagct ggcagaccgt acattttgaa
    10261 caggaagcca ccagacagtt tacccgcagt cgggtcaccc gccatataac gcggaatgtc
    10321 gccgtggaaa acctgacctg ctgcgttggt gtattcacca atctgcatct ggaaaggtac
    10381 gttccagatg tggtgcagac caaacggtac caggcaacgt tcgatgaaac cgtaaatgcc
    10441 aaacgcaact accgggttct ggtaagcagc ccactgagag aaggtctgga ttgcagaacc
    10501 aatcggcggc caaatgaagg acagcacaac gccagtaaag atggcagcca ggcctgaaat
    10561 gatcggcaca aagcgtttac cggcaaagaa gccaagatac tcaggcagct taatacggta
    10621 gaaacggtta aacatgtacg ctgcgatcgc accggagata atccccccga gtacgccagt
    10681 atccgccagg tgtttagagg cgatttcttc agcaggtaaa tgcagtacca gtggcgcaac
    10741 cacggccatg gttttaacca tgatgccata ggcaacaact gcggccagcg cggatacgcc
    10801 atcgttattg gtaaagccga gggcgacacc gatcgcaaaa atcagtggca tgtttgcaaa
    10861 gacggaaccg cctgcttctg ccataacatg cgatacaacg gcgggcagcc agctgaaatt
    10921 cgcggaaccg acgcccagca gaatacctgc gataggcagt acggataccg gcagcatcag
    10981 cgatttaccg accttttgca ggttagcaaa tgcattctta aacataattg agagtgctcc
    11041 tgagtatggg tgcttttttt acgttttcac gcgtggcaag gggggagagc ctcgccgtgt
    11101 acagggcatc taagcgccct ttatttatta cacagagtaa aataattcag tgccgatttg
    11161 tttgacggct atcacgtttc aacttcagat cattggagaa gttgcacatt ttgacaaaac
    11221 atatgtcaca atatttcaaa cgtggttatt caccgcccaa ttttaggcgg tgaactttac
    11281 tcgttttagc cattaattac gagctttaaa aaaactcatt caacggatgg attgaaggcg
    11341 ggaagcgtcg atgtgaaaca gacgggcgaa gttatcggtg gttacctgcg ccagttcttc
    11401 aacggcaaca cctttcaaca cagccatgta ttctgcaacg tcacgaacca tcgcaggttg
    11461 attctctttt cctcgatgcg gaaccggtgc aaggtaaggt gagtcagttt ctaccagtaa
    11521 ccgatcctgg gggacataac gcgcggcatc gcgcagttgc tccgcattac ggaaggtcac
    11581 aatgccggaa aaggagatgt aaaatccgag atccagtaat ttacccgccg tttctctgtc
    11641 ctctgtaaaa cagtgtagta cgccaccgca atccgtcact ttttcttcgc gcagaattgc
    11701 cagcgtatcg gcgcgggcgt cacgggtatg gacgataacc ggcttgttca gttcacgacc
    11761 aatctggata tgatggatga aggattcttg ctgccgtact ttagtttccg gcgtgtaata
    11821 ataatccaac ccggtttcac ccagcgcaac aacgccctct tctgccgcca gacggcgtaa
    11881 atcttctaca tcgtagggat cattctggtt gagtggatgc acgccacaag aaaatacgac
    11941 gttgtcacgt tcgcctacca gatcccgcat gtgtaaataa cccggtaatg ttgtggcgac
    12001 tgccagacaa aatttcacat cgcgtgcggc ggctttcgcc agaacgtcat ccacgtcctt
    12061 atgcaaagat tcataatcca gaccatcgag atggcagtgt gagtcgacta aaaacatgat
    12121 gtctctctta aagatgagga accggtagca caacgcccgg ttgcaggtaa tgctcaatac
    12181 gcagtaaaag atcggtgatg agaagctcgc ggttaatacc tgtgacagac attaactgtt
    12241 cacgaatgtg gcaaacatcc cccagtatag cctgcaggcg cgagggagaa agatggtttg
    12301 ccagttcgac gaccaggccc ggcacatcaa cattggtcac ctgcgcagcg ccatgatggc
    12361 gttttagcgc atccatcagc aacgttgcca gccagtgtaa acgcgccgga gcttgttcat
    12421 gattaagggc cgctagcagc gaataccagt cgcccgatgg cacgctatat gccaaagcct
    12481 gacacaatgt ttcacgagcc tgccagttat ctccgtgaaa caacgccagt gccgcgccag
    12541 gcgaaccggc acttaagcgc aatgcggcaa gtaatgcatc ctgtgacatt gtcacttcgc
    12601 gtgaaagcca ggtcacggcg tactgttccg gcggcggcgc aaggtaatgt aaccgacaac
    12661 gactacgtaa tgttgccagt aaacgttctg gctcgcgggt agcaaggaaa aaccacgttt
    12721 ctgctggtgg ctcttcaagc gttttcagca atgcgttagc cgcggcgtcg gtcagtaagg
    12781 cagcatcggt tacccagaca actttcgcac cacctaagcg tgcgtgctca ttcagctttt
    12841 cggtgacctc acgtaccgca tcaatgccca gcgtattttt ccctttttcg ggagccaggg
    12901 tgtagtaatc gggatgcgtg ccagcctgca tcaactgaca tccacgacag tgaccgcaac
    12961 ttttgtggcc ctgcggttgt tggcagagta aataacggct cagggcgtag attaaagcat
    13021 catcgcccat gcccggtaac gcctgaatga gcagcgcatg gtgacctctt ccggcctgat
    13081 agctggctac cagtttttcg aaatcaggtc gtaaccatgg ataccatctc atgcgtccaa
    13141 ctccttcacc cagttggtca cggtagtgcg gattgcatcc atcacagcct ccagcggcag
    13201 ggtggcatca atggtatgaa tgcttttatc ttgtgctgcc agttccagat agcgggcgcg
    13261 ggtgcgatta aaaaaatcga aagactcttg ctcaatacga tccagctcgc cgcgtgcacg
    13321 cgcgcgtttt aagccaactt ccggggtaac atcgagatag agcgttaagt cggggcgaaa
    13381 atccccgaga acagcatcac gcagtgttgc cagcatatgt tggtcaatac cacgtccgcc
    13441 gccctgatac gcctgagtgg agagatcgtg gcgatcgcca atcacccaag tgccgttagc
    13501 cagcgcaggt ttgatgaccg tttctaccag ttgaacgcgc gcggcataaa acatcagaac
    13561 ttcggcttta tcggtaatga cttcatcgcc taccgatttg atatccagca ccaggcttct
    13621 taacttttcg gcaagttgcg taccgccagg ttcccgagtg aaaaccatgt cgcggatacc
    13681 cagttgctca agcgtctcaa ccaccacgtt acgcgcggta gttttgcctg cgccttccag
    13741 cccctcaatg acgatatact tactgcgcat ttttttcctt aagcactttc agataatcct
    13801 gcacagactt gttatgactg gcaagattgg tattaaacgt gtgaccacct ttaccatcgg
    13861 ccacaaaata gagatacggc gtttttgccg gatgcgcagc agccttcagc gaatccgccc
    13921 ccggcgtcgc tatcgcacct ggcggcaaac cggtaatggt ataggtgtta tacgctgtcg
    13981 gcgtttccag gtctgcacga gaaagtttgc cattataacg ctctcccatc ccgtaaatca
    14041 cggtcgggtc ggtctgcagg cgcataccaa tgcgtaaccg gttgataaat accgaggcaa
    14101 cctgatcgcg ttcactggca acggcggttt ctttttcgat aattgatgcc atcgtcacca
    14161 actggttttt atctttataa ggcagaccgt ccgcacgccc ttcccaggcg ctatcgaccg
    14221 ctttcaccat tttcttgtgc gctcgcttga gtaacgcgac atcggtggta ttggcggtat
    14281 acatccaggt gtctggccag aaccaacctt caatccactc cgggttttcc agttcaagtg
    14341 cctgcgctac ggtggcgtac ttatcgtcgc tcagcgtgtg cttgatatag ggggcctcac
    14401 gcaattgctt gaggtaatcg ctcagacgca tcccttctac cagtcgcaga gggaactgtg
    14461 cttctttacc gctttccagc aatttcagca tctcgcgcac ggtcatctgc ggtgtaaagc
    14521 ggtaagtccc ggctttaaag tgagaaagat ccggttcgat acgcagcagc cattggaaaa
    14581 cccgtgggcg attgatgatc ttatcggcat aaagctgttc accgagcgcc agacgtccgg
    14641 tccctggctt cagggtaaat atcgtctctt ctttgataag taatttgctg tcggcaagat
    14701 ggcgaagctt ccagacgccc acaccagcgg cgatacccag taccaccagc aataacaaga
    14761 taatcaataa cacttttttc atgactaatt cgggcgctca caaagtgggg ctaaatattc
    14821 atataacgtt gctgacgaaa aggagacatc gccacaggca cgtacgggca tcactggcat
    14881 taacgcatta caaataacca tctcatctgc ctgcaacgcc tcctccagcg aggcttgcac
    14941 ttcgacaagc tgataagagg attgtgccag caaacggata cagaattgtc gcataatgcc
    15001 gttaacacct gcctgatcca gtcgcggcgt ataaactacg ttgcccttcc gccagaacaa
    15061 attagccgca cagcattccg taacccaccc ttcgctgtca aggaccagcg cctcatcagc
    15121 gtttgtctgc tcaagatgag agcggatcaa tacttgctca agccgattaa gatgtttaat
    15181 acctgcaaga tgaggattgc gccccagccg caccgggctt agcgccaacg tcatcccctc
    15241 gttacgcaaa cggtcgtaat gtgcgggata aaccgtaacg gagagaatcc gcgttgccgg
    15301 tccgctgttc aatgtgctgt atcctcgccc gccactaccg cgactgatca ccactttcag
    15361 cacaccattt tgctgttctg ctgccagcgt tttcatctct agttcaagct gaggccagaa
    15421 gtcacaggaa atcatcaacc gctgacaagc atcctgtagt cgctggatat gcgccgataa
    15481 caaactgact ttaccgtcga taactctggc ggtggtaaaa caaccatcac caaactgcgt
    15541 tgcccgatcg cttactgcca gcgattcctg cttatgaccg ttaattaaga acataccggc
    15601 tccttatcag agaattgtta gtgtggcagc atgttcacta cggaacaagt cggaataaaa
    15661 gctaagaaaa aaggcccgca agcggacctt ttataagggt gggaaatgac aacttagatc
    15721 tttttaaaga tcaaagaacc attagtgcca ccgaagccga aggagttaca cagcgtgtat
    15781 tccattccgc tgacctgacg tgcttcgtgc ggtacgaaat ccagatcgca accttcatcc
    15841 gggttatcca ggttgatggt tggcggaaca gcctgatcgc gcagcgccag gatggagtag
    15901 atagattcta ctgcacccgc cgcacctaac aggtgaccgg tcatagattt cgtggagctt
    15961 accaacacac ggcttgcagc ttcaccgaag atggttttca ccgcctgcgc ttcagcttta
    16021 tcgccagccg gcgtagaagt accgtgagcg ttaacgtagc caatctgact cgcttcaatg
    16081 cctgcatcac gcagagcatt tgccattgcc agagctgcgc ctgcgccatt ttctggcggt
    16141 gacgtcatat gataagcatc gctgctcata ccaaagccga cgagttcagc gtaaattttc
    16201 gcaccgcgtt ttttcgcgtg ttcgtactct tcaagtacca gcataccggc accatcgccc
    16261 agtacaaaac catcacgctc tttatcccac gggcggctcg ccgcttgcgg gttatcattg
    16321 cgggtagata atgcacgtgc cgcgccaaaa ccaccaacgc ccagcggcgt actggctttc
    16381 tctgcgccac ctgcaaccat cacgtcagca tcgccatacg cgataatacg cgcagcatgg
    16441 ccaatgttgt gcacgccgga agtacaggcg gtcgcgatag agatgcttgg gccacgcagg
    16501 ccatacatga tagtcagatg acctgccacc atgttcacaa tcgttgacgg aacgaagaat
    16561 gggctgatct tacgtggacc accgttcatc agagatgtgt ggttttcttc gatcagtccg
    16621 aggccgccaa tcccggagcc aattgcggca ccaatgcggg ttgcgttctc ttccgttatt
    16681 tcaaggccag aatcctgcat ggcctgaacg ccagcgacaa ttccatattg aatgaaggca
    16741 tccatcttgc gctgttcttt gcgcgagata atgtcctcac agttaaaatc ctttactaag
    16801 ccagcaaatt tcgttgcata ggcgctagta tcgaaatggt cgattaggct gatgccactc
    16861 tgaccggcaa gcagagcttt ccaggtagac tctacggtat tgccgacagg agacaacatg
    16921 cccagtccgg tcacaactac acgacgctta gacacgtttg tcctccaggg agggaaaaaa
    16981 tgattctagt gggacaaaaa gataaaactc aggcggtcga acgaccgcct ggagatgttc
    17041 acttacgcct ggtggccgtt gatgtaatca atggcagcct gaacggtggt gattttctca
    17101 gcttcttcgt ccggaatctc agtatcaaac tcttcttcca gagccattac cagctcaacg
    17161 gtgtcaagag aatccgcgcc caggtcttca acgaaagaag cattgttggt aacttcttcc
    17221 tgcttaacgc ccagctgttc gccgataatt ttcttaacgc gttcttcgat agtgctcata
    17281 ctcttaaatt tcctatcaaa actcgctttc gcgatggttt tcgtagtgta taaaatgttg
    17341 aaaaatttgc aactaaatcc cggcaggtct taccacgatt ttacgttatt ttgaggcctt
    17401 accccccaat aacgcaaatc attttgcact aattattact cataaccacg cagactgcgc
    17461 gcaacatgag cattttgtgc aaatcgcggt cagaccatgt acatcccgcc gttcacatgc
    17521 aaagtttcac ccgtgatgta agctgcttcg tcggatgcca ggaatgcaac cgcgttggcg
    17581 atttcctgtg cgccgccgag gcgacccgca ggaacctgcg ccaggatacc cgcacgctgg
    17641 tcatcgctca gcgcacgtgt catgtccgtt tcaataaagc ccggagcaac aacgtttaca
    17701 gtaataccgc gtgacgcaac ttcgcgcgcc agtgatttac tgaagccgat caagcccgct
    17761 ttcgccgcag cgtagttggc ctgaccgcca tttcccatgg taccaaccac agaaccgata
    17821 gtgataatac gaccatgacg ctttttcatc atagcgcgca ttaccgcttt tgacagacgg
    17881 aaaacagatg aaaggttggt ttcgataata tcgttccact cttcatcttt cattcgcatt
    17941 aacaggttat cacgagtgat accggcatta ttgaccagga tatccacttc accaaattct
    18001 gcgcgaattt tttccagaac agattcgata gatgccgggt cggtcacatt caacatcaga
    18061 cctttgccgt tggcacctaa ataatcagtg atcgcctgag cgccattttc actggtcgca
    18121 gtgccgataa ctttcgcgcc acgggctgcg agcgtttcag caattgcgcg gccaattccg
    18181 cggcttgcac cggttaccag tgcgattttt ccttcaaaat tcatgatttt cctcttttaa
    18241 agctcgagcg ccgctgccat cgctgaaggt tcgttcagcg ccgaggcggt cagggtgtcg
    18301 acaatgcgtt tcgtcaggcc agtaagcact ttgcccgggc cgacttcata gagatgttct
    18361 acgccttgcg ctgccatgta ttcaacagac ttcgtccact gaaccgggtt atacaactga
    18421 cgtaccagtg cgtcacggat ggcatcacca ttggtttcgc atttcacatc aacgttattc
    18481 acaacaggaa ctgttggtgc gttaaaggtg attttcgcta attctactgc cagtttgtcg
    18541 gctgctggtt tcatcagcgc acagtgagac ggtacgctca ctggtaacgg cagagcgcgt
    18601 tttgcgcccg cagctttaca ggcagcgcca gcacgctcaa ctgcttcttt atgaccggca
    18661 ataaccacct gtcccggaga gttaaagttt accggagaaa cgacctgacc ttctgcagct
    18721 tcttcacacg ctttcgcaat agacgcatca tccagaccga tgattgccgc catagcaccc
    18781 gttccttccg gtacggcttc ttgcatgaac ttgccgcgca tctcaaccag acgcaccgca
    18841 tcagcgaaat caatcacacc agcgcaaacc agcgcggagt attcccccag gctgtgacca
    18901 gccatcattg ccggtgcttt accgccctgc tgctgccata cgcgatacag cgcaacagat
    18961 gcagtcaaca gcgctggctg ggtttgccag gttttattca gttcttcagc cggcccctgc
    19021 tgggtcagcg cccacaggtc gtagcccagc gccgcagaag cttcagcaaa cgtttcttcg
    19081 acaattggat agctcgccgc catatcagcc agcattccaa cggtttgaga accctgtcca
    19141 gggaacacaa atgcaaattg cgtcatgttt taatccttat cctagaaacg aaccagcgcg
    19201 gagccccagg tgaatccacc gccaaaggct tcaagcagaa ccaactgccc cggcttaatg
    19261 cgcccgtcgc gtacagcttc atccagagcg cacgggacag aggccgcaga ggtattaccg
    19321 tggcgatcca gcgtcaccac gacattgtcc atcgacatgc cgagtttttt cgctgttgca
    19381 ctgataatac gcaggttagc ctgatgcgga accagccagt ccagttgaga acggtcaaga
    19441 ttattcgccg ccagcgtctc atctacgatg tgcgccagtt ctgttaccgc aaccttgaag
    19501 acttcgttgc ccgccatcgt cagatgaatt gaattctctg gattcacacg gtcagcatta
    19561 ggcagcgtca gcaactcacc atagctaccg tcggcatgca gatgggtgga gatgattccc
    19621 ggctcttcag aggcagccag caccgcagcg cccgcgccat cgccaaaaat aataatagtc
    19681 ccacgatcgg ttggatcgca ggtgcgcgcc agtacatcgg aaccgacgac cagagcatac
    19741 ttcaccgccc cagatttcac gtattgatcg gctacgctta atgcataggt gaaacctgcg
    19801 caggctgctg caacgtcaaa tgccgggcaa cctttaatgc ccagcatgct ttgaatctga
    19861 caagctgcgc tcgggaaagc gtgcgtagca gaagtcgttg ccacaacgat caggccaatc
    19921 tggtctttct caatgcccgc catctcaatt gcgcgtgtcg ccgcttcaaa gcccatggtt
    19981 gaaacggttt cgtttggcgc ggcaatgtgg cgttcgcgga taccggtacg agtgacaatc
    20041 cactcgtcag aggtgtccac cattttttcc aaatcggcgt ttgtccgcac ttgttcgggc
    20101 agatagctgc cagtaccaat aatcttcgta tacatgtacg ctcagtcact tttcggttat
    20161 ataccgtcac ttgcaaactg cgagttcgct ggcagcgtcc tgctaccgca gagttccgct
    20221 tttgccaccg tccagcagct caaaaccagc tgggtataca gattccaggc gagcggcaat
    20281 tcgctgagga acttgtcgct gcaccgcctg cactgcctgt tcaatcgcga ccgcaaaagc
    20341 tcgctgattg gctgcaccat gactttttat caccgtgccg cgcaatccta acagacaggc
    20401 gccgttatac tggtcggggt tgaggtgact gaatcgcctc gtcaggctct tttgtagcca
    20461 acgctttaat aacagtagcc accacgaccg ttttttccct tcaccctgag atttcagcag
    20521 agaaaggaac atcctgacaa caccttccat cgtctttaat gtgacatttc ctgtaaagcc
    20581 gtcacaaacc agcacatctg tcttgccagt taacaactca ttggcttcaa gatagccgat
    20641 ataattgata gaagggattg ttttaagcac cgctgaggca tcccgaatac tgtcgagacc
    20701 ctttacttct tcttcaccaa tattgagcaa cgccacgcga ggattaggaa tttccaccac
    20761 ctcttcagcc agaactgagc ccataatggc aaattgcacc aacattgtac tgtcacaatc
    20821 gacgttggcc cctaaatcga ggaccaccgt tttgcccttt tgctgatgcg gtaataccgt
    20881 caccagcgcc ggacgctcaa tcccctccag gggcttgagt aataattttg ccagccccat
    20941 tagcgccccg gtattaccgg cactgacaca ggcttgcgct cgaccttctt tcaccagctc
    21001 cagtgccacg cgcattgaac tcccacgact ggcgcggata gcttgcgaag gccgggcatc
    21061 actggcgata actgactgcg caggaataat ctgcagacgc gaacgttgtt caaagtcagc
    21121 tttagcaagt aatggcgtga tggcgtcggg attgccgact aaaagaagag tgagttgcga
    21181 attagaattc agtgcctgca atgctgcagg cactgtcacg gaagggccaa aatcccctcc
    21241 catgacatct aacgccaggg ttagacgtgt caaggtatcg tcgccgcccg gtttggtctt
    21301 tccccagttg cctggggaaa atcctcacta agcttcatca cgcagacgcg tgattactta
    21361 gcgatgacct tgcggccgcg gtagtaaccg tcggcagtga tgtggtgacg caggtgtttt
    21421 tcaccagaag ttttgtctac agacaggctg gtgactgcgg tcagcgcgtc atgggaacga
    21481 cgcatgccac gtttggaacg ggttggttta ttctgttgta cggccatgga ccttactcct
    21541 caattacggt ttatccccga tccaacgggg agcaccaatt acttacgctt taagctggct
    21601 aatacggcaa atgggtttgg cttttgcgct tcttcaggca gttcaccaaa gaccatgtcc
    21661 gcttcggaca cttcacagtg ttcagaatca tgcaccggaa ctaccggcaa ggcgaggatg
    21721 atttcatctt caaccattgc aagcagatcg atttcaccga attcgttaac ctcaatcggt
    21781 tcatacgctt ccggcagtgc ttcagcctgt tcgtctgaac gcacaggact aaaacaatac
    21841 gttgtgtaga cctgatgagt aaacggcttc ccgcaacgct gacactcgag cgttaccgtc
    21901 accttcgcat cgccgtttaa cactgcgaga cgttggttat cgatagcgaa cgacatggag
    21961 cattccacat cactgtccac actgactacg gattcggcga cgcgctcaac ctgatcagga
    22021 gtatagatac cctggtaatc aaggcgtttt tgagccgtac gaaccggatc gagagtcagg
    22081 ggtaatttta ccttttgcat agggcgcgca tattaacttt gtaacgtcat agagtcaaag
    22141 aaaaaggcag cctgtggttg ccttttgcca ataattcgca cacattgcgg gttactgctt
    22201 tattttcgtt cagtgctgat aacgaacatc aacctgcaaa acgaagcgca tagtttaaaa
    22261 tggctatcat caatacgcta tatctgatgg aaaaaaatat gcctaaactt attttagcct
    22321 ccacctcgcc ctggcgtcgc gctcttctgg aaaaactgca aatctctttt gaatgtgcag
    22381 cacccgaggt cgacgaaact ccacgcagcg acgaatcacc gcgacagttg gtgcttcgac
    22441 tggcacaaga aaaagcgcaa tctctggcgt cacgttatcc ggatcattta attattggtt
    22501 cagaccaggt atgtgttctt gatggcgaaa tcaccggtaa accgttaacg gaagaaaatg
    22561 cccgtctgca attacgcaaa gccagcggca atatcgtcac cttctatact ggactggcgt
    22621 tgtttaattc ggcgaatggg catctacaaa cagaagtgga gccttttgac gtccatttcc
    22681 gtcacctgag cgaggcggag attgataatt acgtgcgtaa agagcatccc ctgcactgcg
    22741 cgggtagctt taagagtgaa ggatttggca ttacgctgtt tgagcgttta gaggggcgag
    22801 atcctaacac gctggttggt ttaccgctaa tcgcgctgtg tcagatgtta cgtcgggaag
    22861 ggaaaaaccc gctgatggga tagttttctg cgccggatgc ggttacgtcg catccggcaa
    22921 tcggtgctgg cggcctgatg cggcgtaaac gccttatcaa ggccaaccgc ccattatcag
    22981 gtttatatgc ttgtattagc gcgcgttacg cagcttttgc aaacaacgct tcaaaccttc
    23041 atccatcggc gcttcgatac gcatcacctc accggtcccc ggatgggtaa acttcaacgc
    23101 tgcggcgtgc aggaacagac gatttaatcc tgtgcctgct tcagtgagct gtctgtcaaa
    23161 ttcacggtca ccgtagcgat cgtcaaaggc aatcggatga cccgcatact gtgtatgcac
    23221 acggatctga tgagtacgcc ctgttaccgg gctacaacgc accagggtgg caaaagcata
    23281 gcgttcttcc actttaaagc gtgtttccga cggtttgcct tcctgactca cacgcacgat
    23341 acgttcgccg ctttgcagaa tatttttcag caacggcgct tgaacgctct tcacatgcga
    23401 ctgccactga ccgcgcacca gcgccaggta atctttttgc atcccttttt cacgtaattg
    23461 ctcatgcaga gaacgcaacg ccgagcgttt tttcgctacc agcaacacac ctgaagtgtc
    23521 ccggtcgaga cgatgaacca gttcaaggaa ccgcgcttcc gggcgcaacg cccgcaaacc
    23581 ttcgataacg ccgaagctta aaccactgcc gccatgtacc gccgtaccgg aaggtttatt
    23641 cagcaccagg atatggtcat cttcatataa gatgacgtcc gccagcgccg ccaccttttg
    23701 cagatgtggc gaaaccgcct cttcttcccg ttcagcaacg cgaaccggtg ggatacgcac
    23761 ctcatcaccc gcttcgagtt tatattcagg cttaatacgt tttttgttca cccgcacttc
    23821 gcctttacgc aaaatacggt aaatcatact ttttggtacg cctttcaatt gggtacgcaa
    23881 aaagttatcg atacgttgcc ccgcttcgtc agcgttgata gcaacaattt ttacggatgg
    23941 agtctctgtt ttcatggtgg gcgattctaa ttagccaaca ggattcgcgc cactcatttt
    24001 tctatgctta tatttagttt tgcaccttat tacttcacta cgtgatcact ttattgatgg
    24061 ttattaaacc aatcaccagc aagaagtgaa aaaactgtga gtaagcgggt gataaatggt
    24121 aaaagtcatc ttgctataac aaggcttgca gtggaataat gaggccgttt ccgtgtccat
    24181 ccttgttaaa acaagaaatt ttacggaata acccattttg cccgaccgat catccacgca
    24241 gcaatggcgt aagacgtatt gatctttcag gcagttagcg ggctgcgggt tgcagtcctt
    24301 accggtagat ggaaatattt ctggagagta atacccagtc tgtttctttg ataattgcgc
    24361 tgtttttccg catgaaaaac gggcaaccga cactctgcgc ctctttgagc tgacgataac
    24421 cgtgaggttg gcgacgcgac tagacacgag gccatcggtt cacacccgga aaggcgttac
    24481 tttgcccgca gcttagtcgt caatgtaaga ataatgagta agttacgatg aaaagaatgt
    24541 taatcaacgc aactcagcag gaagagttgc gcgttgccct tgtagatggg cagcgtctgt
    24601 atgacctgga tatcgaaagt ccagggcacg agcagaaaaa ggcaaacatc tacaaaggta
    24661 aaatcacccg cattgaaccg agtctggaag ctgcttttgt tgattacggc gctgaacgtc
    24721 acggtttcct cccactaaaa gaaattgccc gcgaatattt ccctgctaac tacagtgctc
    24781 atggtcgtcc caacattaaa gatgtgttgc gtgaaggtca ggaagtcatt gttcagatcg
    24841 ataaagaaga gcgcggcaac aaaggcgcgg cattaaccac ctttatcagt ctggcgggta
    24901 gctatctggt tctgatgccg aacaacccgc gcgcgggtgg catttctcgt cgtatcgaag
    24961 gcgacgatcg taccgaatta aaagaagcac tggcaagcct tgaactgccg gaaggcatgg
    25021 gacttatcgt gcgcaccgct ggcgtcggca aatctgctga ggcgctgcaa tgggatttaa
    25081 gcttccgtct gaaacactgg gaagccatca aaaaagccgc tgaaagccgc cctgctccat
    25141 tcctgattca tcaggagagc aacgtcatcg ttcgcgcatt ccgcgattac ttacgtcagg
    25201 atatcggcga aatccttatc gataacccga aagtgctcga actggcacgc cagcatatcg
    25261 ctgcattagg tcgcccggat ttcagcagca aaatcaaact gtacaccggc gagatcccgc
    25321 tgttcagcca ctaccagatt gagtcacaga ttgagtccgc cttccagcgt gaagttcgtc
    25381 tgccatctgg tggttccatt gttatcgaca gcaccgaagc gttaaccgcc atcgacatca
    25441 actccgcacg cgcgacccgc ggcggcgata tcgaagaaac cgcgtttaac accaacctcg
    25501 aagctgccga tgagattgct cgtcagctgc gcctgcgtga cctcggcggc ctgattgtta
    25561 tcgacttcat cgacatgacg ccagtacgcc accagcgtgc ggtagaaaac cgtctgcgtg
    25621 aagcggtgcg tcaggaccgg gcgcgtattc aaatcagcca tatttctcgc tttggcctgc
    25681 tggaaatgtc ccgtcagcgt ctgagcccat cactgggtga atccagccat cacgtctgcc
    25741 cgcgctgctc cggtaccggt accgtgcgtg acaacgaatc gttgtcgctc tctattctgc
    25801 gtctgatcga agaagaagcg ctgaaagaga acactcagga agttcacgcc attgttcctg
    25861 tgccaatcgc gtcttacctg ctgaatgaaa aacgttctgc agtgaatgcg attgaaactc
    25921 gtcaggacgg cgtgcgctgt gtgattgtgc caaacgatca gatggaaacc ccgcactacc
    25981 atgtgctgcg cgtgcgtaaa ggggaagaaa ctccaacctt aagctacatg ctgccgaagc
    26041 tgcatgaaga agcgatggcg ctgccgtctg aagaagagtt cgctgaacgt aagcgtcctg
    26101 aacaacctgc gctggcaacc tttgccatgc cggatgtgcc gccagctcca accccagctg
    26161 aacctgccgc gcctgttgta gccccagcac ctaaagctgc aacggcaaca ccagcatctc
    26221 ctgcacaacc agggttgttg agccgcttct tcggcgcact gaaagcgctg ttcagcggtg
    26281 gtgaagaaac caaaccggcc gagcaaccag caccgaaagc agaagcgaaa ccggaacgtc
    26341 aacaggatcg tcgcaagcct cgtcagaaca accgccgtga ccgtaatgag cgccgcgaca
    26401 cccgtagtga acgtgctgaa ggcaacgata atcgcgaaga aaaccgtcgt aatcgtcgcc
    26461 aggcacagca gcagactgcc gagacgcgtg agagccgtca gcaggctgag gtaacggaaa
    26521 aagcacgtac caccgacgag cagcaagcgc cgcgtcgtga acgtagccgc cgccgtaacg
    26581 atgataaacg tcaggcgcaa caagaagcga aggcgctgaa tgttgaagag caatctgttc
    26641 aggaaaccga acaggaggaa cgtgtacgtc cggttcagcc gcgtcgtaaa cagcgtcagc
    26701 tcaatcagaa agtgcgttac gagcaaagcg tagccgaaga agcggtagtc gcaccggtgg
    26761 ttgaagaaac tgtcgctgcc gaaccaattg ttcaggaagc gccagctcca cgcacagaac
    26821 tggtgaaagt cccgctgcca gtcgtagcgc aaactgcacc agaacagcaa gaagagaaca
    26881 atgctgataa ccgtgacaac ggtggcatgc cgcgtcgttc tcgccgctcg cctcgtcacc
    26941 tgcgcgtgag tggtcagcgt cgtcgtcgct atcgtgacga gcgttatcca acccaatcgc
    27001 caatgccgtt gaccgtagcg tgcgcgtctc cggaactggc ctctggcaaa gtctggatcc
    27061 gctatccaat tgtacgtccg caagatgtac aggtcgaaga gcagcgcgaa caggaagaag
    27121 tacaagtgca gccgatggtg actgaggtcc ctgtcgccgc cgctgtcgaa ccggttgtta
    27181 gcgcgccagt tgttgaagaa gtggctgaag ttgtagaagc ccccgttcag gttgccgaac
    27241 cgcaaccgga agtggctgaa acgacgcatc ctgaagtaat tgccgccgcg gtaactgaac
    27301 agccgcaggt gattaccgag tctgatgttg ccgtagccca ggaagttgca gaacacgcag
    27361 aaccggtggt tgaaccgcag gaagagacgg ctgatattga agaagttgcc gaaactgctg
    27421 aggttgtggt tgctgaacct gaagttgttg ctcaacctgc cgcgccagtc gtcgctgaag
    27481 tcgcagcaga agttgaaacg gtagccgcgg ttgaaccaga gatcaccgtt gagcataacc
    27541 acgctaccgc gccaatgacg cgcgctccgg caccggaata tgttccggag gcaccgcgtc
    27601 acagtgactg gcagcgccct acttttgcct tcgaaggcaa aggtgccgca ggtggtcata
    27661 cggcaacgca tcatgcctct gccgctcctg cgcgtccgca acctgttgag taataattag
    27721 ctcaagtaat caagccctgg taactgccag ggctttttta tttcaggggt agcatctttg
    27781 ctgtaatgac attagcgtta catgtactgt ataattatca tccacgcaaa gccgaaggat
    27841 atatttatgc cagcagccaa taacattcca gccaggcgcg aatctttaaa cttgcgcata
    27901 aaacccgcag aacgcgatct tattgaccgg gccgctaaag cccgaggaaa aaatcgcacc
    27961 gaatttgtcc ttgaggccgc cagagccgca gctgaagagg cgctcattga gcagagaatt
    28021 ttcaggatcg attctgatga ttatcagcta tttttgaatc gtctggatca gccccccgcg
    28081 cccaatgctg cattacgcaa aaccatgcaa acacccgcgc cgtgggagca aaaaaaatga
    28141 ttcaggcccc cgagccactc atggcaaggc atcaattcac ctctttttgc tcgggagtag
    28201 aaacgatgga taactggcta aagcagagag ccctaaaaaa tcagcttgcc ggcgcatcac
    28261 gtacatttgt tagctgtgat acgtatagca acgttttggc atattattcg cttgcttcca
    28321 gcgccgttga aacgtatgtc gcgacaggac gatttcgccg aaatatgcca gaaccgatcc
    28381 ctgttgtagt acttggtcgt ctggcaatag ataaatcatt acagggtcag ggcatcgatc
    28441 gtgcgatggt cagagatgcc ggtctacgag tactacaggc ggctgaagtc attggtattc
    28501 gcggaatgtt ggtccatgcg ttatcagacc aggcccgtga gttttatctg cgagtggggt
    28561 ttgaaccatc gcctgtcgat tctatgatac tgatggcaac attggctgat ttgcaggaat
    28621 gtcttaaata gttcgttatg tctcatgcaa aaaaatgccc gccccggcga tcccagagca
    28681 ggcagacaaa acatacccag tttcatgata tgttttaaag cgaaattatt tgttgagctg
    28741 gaagagcgac aatccctgca tatcggtaaa tgctttatac gacgcctgca atgccgtttg
    28801 ctgcatgatg taagatgaaa tagttgcatt ccagtccaca tcaaccagat cgctcatctg
    28861 ctgcgtttgc cctaaagcgc gatcgctacc taatgaatcc agcgactcca gttcgttcag
    28921 ctgcgtgcct aattccgcgc gaacagtcag cacattgttc agcgagtttt tcagtccgcg
    28981 gttggtttta tccagtgccg cagcggcggt ttctttatcc gcttcgctat ccgcgaccgg
    29041 cgttttcagg gctgcgatgg cgctatccag catggcaaaa agattggttt cagaagcgct
    29101 accgtctggt tccgctaccg cgttgctggt aatactgtcg aaaattttgt cacccgtgtg
    29161 ccctatcacc atcgaacgcg aagcatcgac ctgttgttta atactttctg ctccgccttc
    29221 gtattttcca tccgcctcgc taaacggtgc ggattctgtt ttataaccgg caaaaatgta
    29281 gcgcccgtta ccgtcagtgg tgttcgccag attcagcaac tggtcacgaa gcccctgaat
    29341 atccgtagcc agcgaggccc ggtcatcgtc actcaaggtg ccattgctgg cgtagacaat
    29401 tttttcctga gcattctgga tagcggtggt gacctggcta agtacactct cttccagtga
    29461 cactttttga gtggcgaaag tacgcgccag cgtgtactgg ctgttttgcg cctgtgcctg
    29521 ggagagaaca acagcttgtg atgcagcaat gggatcatct gaagggttaa cgactcgctt
    29581 acccgtcgac atctgttcgc cgtacttcat ccattctgcc tgagaattgg tgataccacg
    29641 catgttttgc tggtacatca tctgggtact gaaacgcatc ttatctcccc ttagcgaatg
    29701 ttaatcagcg catcaaaaat cgcgtttgcc gtctgcagaa cctgcgcatt cgccaggtaa
    29761 tactgctgaa aacgttgcag atttccgtac tcctcatcga gattgacacc ggaaatcgac
    29821 tgctgctgat tggaaagctg cgtcaccaca ttaccttgcg tggtgctact ggttttcaac
    29881 gtcgcggttt tattaccgat atcactcact aacgaggcat aagcgtcgtt aaaggatttc
    29941 gcaccgccca ccgttttact gttgctttgc agatcgagca gggcctgacc gttgcggttg
    30001 tcgctatcac ctgtatcttc tgcgctcgcc atggcgattt tcgcttcgtc ggtgattaat
    30061 acatccatgt tgacgatggc gtcacttacc ggtttcagcg tgaagctgtc gttaacggca
    30121 ggcgttcctg taaacgtcaa ctccagacca tcaaatgcca ctttaccgct ggcatccggc
    30181 gtcaccgtaa aagtggtatt actggcaagg cgggtgacct gccactgatt attatcgaac
    30241 gagattttgt aatctgtcgc cagtaccgcg gaggcatcag ttaccgttgc accgatcgca
    30301 acgtcacctt tgtttttcgt gttttgcaga accgcgggct taccgatagc aaagaaatct
    30361 tcaccggcat cgccgttagc atcaaatccg gctttgtgtt gggtgttgaa agcctcggca
    30421 aatgccagcg ccagttgtcc aagcgtatta cgcgtctggt ccagatcctg agaacggaat
    30481 gtcagaatgc cgcccagcga cccggtattc agtaatttct ccgggatctc aatattgcct
    30541 gccgtcccat caacataagc gacagtcgta cgagaagggt cagcgctgga aggaactgcc
    30601 gccagttgcc gagccgtact tccctgaacc agtgagtaac cattggccat cgtgatgtta
    30661 taagtgccac catcctgaac gctgacttct acaccaacaa tctggtttaa ttcgctcacc
    30721 agttgatcgc gttgatccag cagattgtta ggtgacgccc ctgcccccac gcctgtcagg
    30781 cgggagattt gatcgttcag gctggcgatt tgtttagcgt aattgttgat ctgatcaacg
    30841 ctggcaccta tcgcgatatt gacctgtttg tcctggtcgc gcagatattg atcggtggtt
    30901 ttaaactgat tcaccaaccc ttctgatttc ccaatcagcg cctggcgcgc tgccggatct
    30961 tccgcgttac tcaccagcgt ttgcaggctg gtgaagaaat cctgcatctg tgttgccagc
    31021 gaagaggtac tggtggagag catattgtcg attttcgaca tctgctcata gcgggcggtc
    31081 aggccgctac tttgcgtctg cgcagcacgt aactggttgg taataaacgc atcatactca
    31141 cgctgcacac cagaaacgta gacgccattg ccaacccagc cgccagcgcc caacgtgcta
    31201 ttggcctgcg ccataatagt ggtttggcgg gtatatccgg caacgttata gctggagata
    31261 ttattacttg ccgtatttaa cgccgcctgg gccgcgttca gtccgctcat ggcgttattg
    31321 atcaagctgg acatggaggt tccttataag ccttcaatta cagagtatta tcggcagcgc
    31381 cccgccggac ttgagttatt cagaacagat tatcaatgtt catgctgtag gttttgctca
    31441 ccttgtcgct tatcgatttc atctgctgaa tcatgctggt gagtttgcgg gcatagtgag
    31501 gatcggtggc atagcccgcg tcctgtaggg cctgcgcccc ctgctccgca ctcgcggcgg
    31561 tcgtcacggc ggcgtagcgc gggttacgcg ttaacagccc aacgtaatcc gacaatgctt
    31621 ccagatacga gctgtagacc cgaaacttcg cttttacttt cttcgcttcg ccattttcat
    31681 attcagtcgt ggtgatttca gtgactggcc ctttccagtt gccagaggct ttgacgccaa
    31741 acaggttata gctcggctcg ccgttttcac ggcggatttg ccgttgtccc cagccagatt
    31801 ccagcgccgc ctgagcgagg atcaaatgat gtggcacacc gctttgctgg cttgccagtt
    31861 gggcgggcaa cgagagttgc gcgaggaatg ctttactgtc acccggcagc gaatcatcgt
    31921 agttacgtgg tacggccttt tgcaccagct gcgaaagcgt ctgattttga taacgcacca
    31981 cggtttcgag cgggaatttc atcggtgctg ctggcgtgga ctcctctggc aatggttgtt
    32041 ctggcgtcat ctgtttaacc atcatctctg ccagccccag acctttgccc gccgtcatct
    32101 gttgggcaat ctgctggtca tacatactgg tatacaggcg agtgtgctcg ctgctgaaca
    32161 ggccatcttt tggtaaagcg tcgcgcatgc ttttcaacat catctgcacg aacatccctt
    32221 ccacctggcg ggccaccgga cggatatttg ccgccggatc ttcgcccgct ttcgccttaa
    32281 gttcgttgag cgattgtgca tcccaggccg cacttgccag tagtttgctg tcgctgatca
    32341 tcagatgatt tccagttttg cccgcagaca tcccgcactt tgcattgatt gcaaaataga
    32401 catcagatcc atcggcgtag cgcccagcgc attgagcgca cgcaccacgt tattgaggct
    32461 ggcgctggaa cgtacgcttt gcagcgaacc gccgctctgg cgtaaatcga tctgcgtttg
    32521 tggagtaacc acagtctgtc caccaccaaa cggtgtatct ggctggctga cattggcctg
    32581 gcgattaact gttactgaga gatttccctg cgctaccgcg cagctgtcga gggtcacttc
    32641 gcgattcatc accaccgaac cggtgcgcga gttaatcact actttagcgt cctgcggggt
    32701 aacattaacc tgcatattct ggatatcggc aaggaaacgg acctgggaac tgttgccact
    32761 cggtacgcgc acctgaatag tccgcgcatc caacgcggtg gcgctgccat atccacgcac
    32821 tcggttgatg gtgtcagcga tttgctgtgc catgctgaaa tcttcgtcgt taagttgcaa
    32881 attaagggta ttcccgacgc caaactggct gggcaattca cgttcaataa ccgcaccatt
    32941 ggtgatccgt ccaccgttca gttggttcac ctgaacactg ctaccgccag cggaggcccc
    33001 tgcgccgcca accagaatat tgccctgcgc cagcgcatac acctgactgt caacgccctt
    33061 aagcggtgtc atcaataacg taccgccacg cagacttttg gcatttccca tggaagaaac
    33121 caccacgtca atggtttgcc cctgacgtcc aaacggtgga agtgacgctg tcaccataac
    33181 cgcagcgacg ttttttagct gcatattagt gcccgtcgga acggtaattc ccagctgtga
    33241 gagcatgtta ttaagcgttt gtgtggtaaa cggcgtctga gttgtctggt caccagttcc
    33301 atccagcccc accaccaggc catagccaat cagtgagttt tgccttaccc cctgaacact
    33361 ggtgagatcg cgaatacgct cagcctgagc cgccgtcgta accagtagaa gaattaatgc
    33421 agagagaaat ttaatcacca cagcctcact tacattggag acaggttaag aaagaaacgc
    33481 tgcaaccagc ccatattttg cgcttcgtta atgtagccat tgcctacgta ttcaatgcgc
    33541 gcatccgcca cctgagtaga cggtacggta ttgctgccgc tgatagtgcg tggattaacc
    33601 acgccagaga agcgaataaa ttcggtaccc tgattaatgg cgatctgttt ttcacccacc
    33661 acatgcaggt tgccgttaac cagtacctgg tcaaccgtca ccgtcaacgt gccgctaaag
    33721 gtattactgg cattggcccc gccttttccg ttgaacgtgt taccaccgga ggcttcgaca
    33781 tcggcacgag cgttaccaaa cagcccctgc aaatagcgcg gcacagtatc aaagccaaaa
    33841 ttagttttac cgtcacggct ggcattcgca gaggagcttt tgctggcgct gacgttctcc
    33901 tgcaacacga tggtcagcgt atcgccaata ttgcgtggtc gacgatcttc aaacagcggt
    33961 tgatagccat agttaatcgg ctgagcagac tggaaaatag aaccgttggc gacgggcgtc
    34021 ggaccgggaa ccggttgtgc actggtcgcc ccctgtacca gcggcgtgga gggtatccag
    34081 gcgcagccgg ttagtgaaag caccaacaag ctggaaatgg cataagtatg cgcagcgttt
    34141 ttttgcatgg ctatcatctt caaaaatcag taaaccggtg aatctgccac cggttatgcc
    34201 ttagagttgc gtcagttttt gcagcatctg atcggtggtg gataccgctt tactgttgat
    34261 ttcgtaagcg cgttgcacct gaatcatatt gaccagttct tccgccacgt tgacgttaga
    34321 cgtttcaaca tacccttgat acagcagtcc cgcgccgttc aggcccggcg tgctttcgtt
    34381 cggtgcaccg gaggattgcg tttcggtgta gaggttttcg ccaatactct ccagtccggt
    34441 atcattcata aaggtggtga gattgagctg cccaacctga accggagctg cctggccttg
    34501 ttgtgttacg ctgaccacgc catcacgacc gatggtgata cttagcgcat tcgccggaat
    34561 ggtgatcgct ggctgcacct gaaaaccacc tgccgtcacc agctgcccgt tctgatccac
    34621 ctggaaagag ccgtcacggg tataggctga tgagccatct ggcaacatca cctggaaaaa
    34681 gccctgccct ttaatcgcga catctttgct gttgttggtc tgcgacagat tgccctggct
    34741 gtgtaagcgt tcagtggcga ccgggcgtac acccgtgccg atttgtaatc cggatggtaa
    34801 ggtggtttgt tcggaagact gagcacctgg ctggcgaatg gtttgataaa gcagatcttc
    34861 aaataccgcg cgctgacgct taaaaccgtt ggtactgacg tttgccaggt tgttggcaat
    34921 gacgtccata ttggtttgct gggcatccag gcccgttttg gcgatccata atgaacttat
    34981 cataaaatgt cctgtagtgg attagtgaca aagtgcatct gtcttcttgc cggatgcggc
    35041 gtaaacgcct tatccggcct acaagagctc gcgaaatcaa taaattgcga ttgccccata
    35101 gacccgataa acttgcgcat cgggcaactt atacttgtca tgtatccttt caattaactc
    35161 atcgacagca gttggttggc tcggcctgcg ttatcatcga cgctgctgat caccttcatc
    35221 tgcatttcaa aacgccgcgc gctggcaatc atgtcgctca ttgccgcaac ggcattgacg
    35281 ttactgcctt ccagaacacc cgacatcaca cgcaaggttg gatctgcctg cagtaccggc
    35341 ccacgcgtgg tctgggtttc tgcgcttaaa cgaaaaatgc cgtcgtcacc gcgctggact
    35401 tcgctgcccg tggctttcac cagtttcaga cgccccactg gcgcaaccgt atttgccgga
    35461 tcgcccggat tcagcgccga gattgtgcca tcggcagcaa tagtgatttc cgccccttcc
    35521 ggcacagcaa ttggcccagc ctcgcctatc accggatgtc cctgaattgt cagttgcccg
    35581 gtgggatcaa cctgaatgct gccattacgc gtataccctt cgctaccgtc agcggtctgc
    35641 acggccagcc agccatcctg ctgcaacgca acgtccagcg ggcgcgaggt gtaatccatc
    35701 ttgccgggcg tcatgtctgc acccggcgtt gacgccgtta ccagcgtgcg cgtaggcagt
    35761 gaaagccctt ccaccggcac tgcgcgcaag gcgtttaact gcgcgcgaaa accgggcgtt
    35821 gaggcattgg ccagattact ggcggttacc gcctgttgat tcaacgtctg gctggctgct
    35881 cccatcgcgg tataaattgc gtgatccatt gagctatccc gtcagcgatt agcgtaagtt
    35941 aaccagcgtg ttgaggatct ggtcctgggt tttgatggtc tgggcgttag actgatagtt
    36001 acgctgagca acgatcatat tgaccagttc tttactgaga tcgacgttgg acgcttccag
    36061 cgcaccgttg gtcagggtgc caaagttgcc cgtcccggct gtccccaaca gcgccacgcc
    36121 agaagattgc gtcgcagacc agacgttgtc gccttcggat gccagacctt cgttgttggc
    36181 aaagttcgcc agtacaatct gccccagcag ttgggtttgt tcgttggaat agttgccgac
    36241 aaccgtaccg tcatcattga tttgataact caccagatcg cccggtttgt agccgttctg
    36301 ggtggttgcc acaatgttgt tagcgccggt attttgctgc atggagttga ggaagctcag
    36361 gctaaacgtg gcgggttctg caccgttaat tgcgccagtg gaaatattcg ctgttggatc
    36421 tgaggtcaga acgccattgg cattaaacac cagcgtcatt gcaggctcag ctgtacctgt
    36481 tggatcactg ctatcctggg tgtagacctg ccagttatta tccccggtct tcacaaagta
    36541 gacgctcatg tcatgagcat taccctgact atcgaaaaca gtcaccgaac cttttttgtt
    36601 atagctatcc gcattgctag catcaaatgc gttaacagag ggaagcggat cactggaatt
    36661 caggttgatc tgcatcgacg ccgtggtggt agttttcgct gccatcaggg tattcggaat
    36721 cgaaatattg gtcggattcg ccccttgctg aatagtcggc ggcgtaccgg ttgccgggta
    36781 acccgtcagc tgtaaacctt gcatattcac caggttacgg ttttcatcca gcttaaattg
    36841 tccgttacgg ctgtagaaca ccgaaccgtt gctgtctacc agacggaaaa aaccgttctg
    36901 gctgatagca acgtccagac ctcgcccggt gttggtggtc gtgccatcgg taaagtcctg
    36961 agtgatacca gcaactttta cccccagtcc cactttcgaa ccggcaaaca tatcggcaaa
    37021 agaggccgtg cctgatttaa agccgtaggt ggcagagttg gcgatattgt tgccaataac
    37081 atcgaggttg gtggcggcag cgtttaatcc gctaaccgct tgagaaaagg ccatgactga
    37141 ctcctgaaat gtgaaggctt aaattatctg ccgtacttcg tcgagggtgg tggtgccgta
    37201 agtgccgaga tccagcgtat taccgttgtt gccgcggatc acaccctgca ccagagcaaa
    37261 ctgcagcggc tgggcaacca gttgtgtacc accgttactg gcgctaatcg ctacgttgta
    37321 agaaccgttc ggcgcagtgg tgccgtcagt caacgtaccg tcccaggtga aactgtggac
    37381 tccggcggtc agttcaccaa tatcaatggt acggacaacc gcaccgtttt tatcggtgat
    37441 ggtggccgtc actttgtctg ccgcctgttg cagctcaaca ccaaacggcg tggtcgtggt
    37501 cacagcccct tcttcactgc cggttcccgc aagaacagtg gtgccgggga tcatcacgcc
    37561 gtgaccgatc aggttactgg cctgtaacga ctggctgtta tcaatctgtc cggaaataga
    37621 tccgagcgtg gtattgagtt tttcaatccc gctgaccgtg ctgatttgag ccaattgcga
    37681 cgtcagctcg ttgttttcca ttggattggt cgggtcctgg tttttcagct gcgccaccaa
    37741 caaagtcaga aaactgcttt gtaaatctgc ggcgttgctg cccgtgagcg aactactact
    37801 ggtggtgctg acgccggtat ttgtcggatc ggtggtggtt accgcaatgg acatagtttt
    37861 ctcctttatt gaccgagcgt aagggttttc agcatcatgc ttttcaccgt gttgagcact
    37921 tcaacattgg cctgatagct gcgtgacgcc gacatggtgt taaccatctc tccgacgaca
    37981 tcaacgtttg gcattttcac gtagcccttt gcatccgcca gcggattacc cggttcataa
    38041 accagtttgt ccggggcctg actttctata acatcggcaa cctttacgcc gcctgtcgca
    38101 gcacctggcg cagcgttaac ctgaaatacc acctgttttg cccgatatgg ctgtccatcg
    38161 ggaccggtca cgctatcagc attcgccaga ttactggccg ccacgttcag gcgctgggac
    38221 tgagcagtta acgccgaccc ggcgatatca aaaatattca gcagtgccat ccgttaattt
    38281 ccgctctgta aaacgttcat catgcctttg atttgcccgc tcaacgcgct aaggctcatc
    38341 tggtattgca ggctgttatc ggcaaactgg gtgcgttcgc gatccatatc gacagtatta
    38401 ccatccagcg agggctggtc cggaatacgg tattgcagtt ctgcggtagg aggcgtcagc
    38461 gcctgcgccg gaatgtgttg cgttgaggtc atcgtcagtg caaccacgct tgttgcatcc
    38521 cgtccacgtt gcatgacttt tttaagttca ctggcaaaat cgatatcgcg cgcctgataa
    38581 ccaggggtat cggcattggc gatgtttgct gccagcactt cctgacgctg ggcgcgcaga
    38641 ttgagcgcct cttgttgaaa acgtaaggcg gcgtcgagct tatcgagcat atctcctccg
    38701 caggtatcaa aattcggcca tcagcttaaa tgcctttacg cgcgcgttat cggcggaata
    38761 aacgcaaaat gggtcgctat ttatgccgtt gatggtcatt gcggacaggt acaattcacg
    38821 ttgtagaaat ggctgggggg cgaaaatgct ggcaataaaa cgtagcgtgg cgatcatcgc
    38881 gatactgttc agtccgttaa gtgcggcgag caatctcaca tcgcaattgc acaccttttt
    38941 tagcgcccag ctcgcggggg taagtgatga ggttcgtgtt tctattcgta cagcgcccaa
    39001 tctactaccg ccgtgcgagc agccattgct ttcgatgagc aataattccc gcctgtgggg
    39061 caatgtgaat gtgttggcac gctgcggtaa cgacaaacga tatttacagg ttaatgtgca
    39121 ggccacagga aattatgtgg ttgccgcaat gcccattgtg cggggaggaa agctggaagc
    39181 tggcaatgtc aaactgaaac gcggacggct ggacaccttg ccaccgcgta cggtgctgga
    39241 tatcaatcaa cttgttgatg ccgttagcct gcgcgatcta tcacccgatc aacctatcca
    39301 gttaacccag tttcgccagg catggcgggt aaaagcggga caacgcgtca acgtgatcgc
    39361 cagcggtgat gggtttagcg ccaacgcaga aggtcaggcg ctgaacaatg ctgccgtcgc
    39421 acagaatgcg cgggtgcgca tggtatcggg acaagtggtc agcggcgttg ttgatgcaga
    39481 tgggaatatt cttataaacc tgtaagctgt taaagattac ccgtcccttg ccgataaata
    39541 agcaacacat gataaaagcg ccctcaatga ggaataaacc atgagtattg atcgcacttc
    39601 gcctctgaag cccgtaagca ccgttcaacc gcgcgaaacc actgacgcgc cggtaacgaa
    39661 cacccgggcg gcaaaaacaa ccgcctccac cagcaccagt gtgacgttaa gcgacgcgca
    39721 agcaaaactg atgcaacccg gcagcagtga tatcaatctt gaacgtgtcg aagcgttaaa
    39781 actagcgatt cgtaacggtg aactaaaaat ggacaccggc aaaattgccg atgcgctgat
    39841 caacgaagcg cagcaagact tgcagagtaa ctgatcgtat gacgcgtctt gcagagatcc
    39901 tcgaccagat gtccgctgtg cttaacgatc tcaaaacggt aatggatcaa gagcagcaac
    39961 atctctctat ggggcagatc aacggcagcc agttgcaatg gattacagaa caaaaaagct
    40021 ctctgctggc gacgctggat tacctcgaac agttacgcag gaaagaaccc aatgcagcaa
    40081 atagcgttga tattagtcaa cgctggcagg aaattactgt gaaaacgcag caactacgcc
    40141 aaatgaatca acataacggc tggttactgg aaggacagat tgagcgcaat caacaggcgc
    40201 tggaaatgtt gaaaccgcat caggaaccga cgctatatgg agcgaacggt cagacctcaa
    40261 caacccatcg cggcagtaaa aagatttcga tctgaagatt atctccggcc tgcactgcag
    40321 gccggaatgc ctttttacac cgtccggcgg gcaaactctt taactttgaa gcccagtacc
    40381 gccagtgctg caaagtacgc ggcaatcccc gccagcacga ccgccattaa acgcagtaaa
    40441 cgccagggca tggtacccaa tgaccactcc ggcatgatat gtaacatacc taaaagcacg
    40501 ccagacatca ccagtaccgc caccaccaga cgcaacagaa acgccatcca gccgggttgc
    40561 ggggtaaaga ttttctgctt acgcaactgc cagtaaagca gcgaagcatt cagacacgcc
    40621 gccagaccaa tagaaagtga cagcccggca tgtttcaacg gaccgataaa cgccaggttc
    40681 atcaattgcg ttaaaatcag cgtaacgatg gcaattttca ctggcgtttt aatgtcctgg
    40741 cgggaataaa agccaggagc caacactttc actacaatca ggccaatcaa acccaccgag
    40801 taggcaatta acgcccgctg ggtcatcagc gcatcaaagg cggtaaattt accgtactgg
    40861 aacagcgaaa cggttaacgg acctgaaaga atgcccaacg caaccgcact cggcagcgcc
    40921 aacaggaaac aaagacgcag cccccagtcc atcaaacggt tgtattcatc atgattgcca
    40981 ctggcaaaac ttttcgacag cgacggcagc aaaatggtgc caagcgccac gcccagtaca
    41041 ccggacggaa actccattaa gcggtcggcg taatacatcc aagacaccga accggaagca
    41101 agaaacgagg caaaaatggt gttgatgatt aaggagatct ggctcacaga gacgccaagg
    41161 atcgccggtc ccatctgttt caccacgcgc attgctccgg catcgtggaa gttaatgcgc
    41221 ggcaggacca gcatgccgat cttcttcagg tgcggtagct gatacaccag ctgcaggatg
    41281 ccgccgaccg taacagccca cgccagcgcc agcaccggtg ggttaaagta cggtgcggca
    41341 aacagcgcga aaccaatcat gctgatatta agcagtgttg gagcaaacgc cggaatcgag
    41401 aagcggttcc acgtattcag aatcgctccc accagcgacg ccagggagat cagcaagata
    41461 tagggaaagg ttatctttag tagctggctg gtcagggcaa atttgtcggc agtgtcagcg
    41521 aagcctggcg cggtcaccat gatcacccac ggtgcagcga gcatgccagc gaccgtcaca
    41581 accgccagcg cgagtgtcag caggccagaa acataagaga caaagacccg cgtggcgtct
    41641 tcaccctgct tgcttttata ttctgccaga atcggtacaa atgcctggga aaatgcccct
    41701 tcggcaaaga tacggcgcaa caagttagga agtttaaaag cgacgaaaaa ggcgtcggtt
    41761 gccatccctg cgccaaagat tctggcgaca attgcgtcac gtgcgaagcc aagcacacgc
    41821 gaaaacatgg tcatcgagct gacggcggcc agcgatttta ataaattcat cggtgttcta
    41881 atccagaccc acgggcaaaa cgcctgcaaa gcaggcgctg aaagaggcga atagtctacc
    41941 tggattatgg tgaattgcta ccgccagatg ttacagggtt tattcactca tcgcatcgcg
    42001 ccagatcttg tcaacgatac gttgcgcccg cactgcctgt tcgccggcgg tttgcggaac
    42061 tgtctgattt tgcacgcatt caatgaagtg ccgcgcacag ccgacaaatc cacgttgctc
    42121 aagcgtactc tgccaaccag gaatcggttt atgcactacg ccctgcccgc gctcctcacg
    42181 ccattcgcgc atatccgtaa tgtcgatgag cgcaccgtca gtcacggcct gcacggtttc
    42241 acgctggctt ccggcacggc gatgcataca ggtggtgatc tgcaaaggac cggccgaaaa
    42301 atggtgctcg gcaaacagca tttcgccagc gtcgttagtc agtagcgtac cgccatccag
    42361 agaggctttg ccgcccgaca accacagcgc ggtatccacc acatgcagat aatcatccag
    42421 caacgtgaaa taaagatcgt gtggcccgac gctattgcta cgatgtttat ccattcttag
    42481 cgaggctgcg gtggcgagtt gcgtttttaa ctctccgtag agtggtgcga aacgacggtt
    42541 aaaaccgacc atcaacgtca gttttttccg cgccgccagt tccaccaacc gttcagcatc
    42601 gcgcagattt tctgccagcg gtttatcgac acagacatgt acccccgtat tgagtaacgt
    42661 actgaccacg tcaaagtggc tggcggtgct ggaatgcaca aaaaccgcat cgcaactggc
    42721 ggcaaggctg gataacgaat cggcataagg aatgcgccag ctttcacaaa ttggcagggc
    42781 tttcgcgcgc gtaggcgacc aggctccttg taacatccag tcagacgctg ccgccagcac
    42841 cggtaaccac gctttttgcg caatgccgcc taatcccact acgccgatac gtaatttttt
    42901 cactttaatc tcccagatgg gcaagcaacg aatcaagacg ctgtttcagt tctgccactt
    42961 cgatttccag ggcttcgacg cgagcctgta aatcaccgtc aaccgcgttc gacatatcca
    43021 tcaccgccgg ctgatcttca acctcaccac tgaaaagatg catgtagcgg ctttcacgtt
    43081 tacccggttc gcgggccaga cgcaccacaa aaggaccatc ttcgcgattt gccagttgtt
    43141 ccagcgtcga ctccacttcc gccatatcgc tgaattcata cattcgcgcg gcgcggctgc
    43201 gcagttcacc cggcgtctgg gcaccacgca ataacaacgt ggtgattaac gccacttctg
    43261 cggcgctcag tttcagatcg ccaaattctg aattacaaaa acgttgctca tatttggtga
    43321 cccgattacc aaaaccgctc actgtgcgta gataatgacg tttgaccaga ttatccagct
    43381 gttcctgcac ttcggattca ctcagattca tgaccggttc acggttcgtt ttctgattac
    43441 aggccgtgac tacaccattg actgagagcg ggtattgctc cggcgtggtc acctgctttt
    43501 ccagcaagca accaatcacg cgagcttcaa gggcagtaag ttgatatttc atgagattct
    43561 ccttagcggc cgggcgtcca gtctggggta gttaatgccg tcagtacgtg atcgcgccat
    43621 tgtccatcaa tcaacagata gtctttcgca tagccttctt tttcaaaacc cagtcgcgcc
    43681 agtaaatcac cgctgcgttt attgtgcggc atataattag ccataatgcg atgaatatgt
    43741 tgggtgcgct gcatataacg aatggctgcg gtcagggctt caaacatgag tcctttgccc
    43801 tgccattttt gcccaatcga ataaccgaga tagcaggcat gaaaagagcc acgaacaaca
    43861 ttggaaaaat tggcaacgcc aataatctct ttttcgtccg ggtcgaataa gccaaagtag
    43921 aaagctgaac cttgtttatg aaattcgtta atcatcccta gcctggcctg ccagcctgat
    43981 ggataacagt ggctttcgtc gcgcactggc tcccagggct tgaggaaatg gcgattctct
    44041 gcgtaataat ccgccagacg ccaggcatca cgatcatgca ccagacgcac gaccagtcgg
    44101 tctgtggtta agcgcacttt tggcacgtta ctgcgatagc caaacattct atacctactc
    44161 cttcccgtaa cgtctttaat acgctgtaag tattactata cctgcgcaac cagaccctgt
    44221 gaaaagtcca gaatgccatt ttctgcatta ttcacatatt tgataaaaac tctctatcaa
    44281 agcgacgttt tgataaaaaa atattgtccc cgaagtgatg ggaaaagtct tagcggcaat
    44341 gcagaataga aagtgctcat cttttatttc ccgggagggg aaatgtcccg cgtgtcgcag
    44401 gccaggaacc tgggtaaata tttcctgctc atcgataata tgctggtcgt gctggggttc
    44461 tttgttgtct tcccgctgat ctctatccgc ttcgttgatc aaatgggctg ggccgccgtc
    44521 atggtcggta ttgccctcgg cctacgccag tttattcagc aaggtctggg tattttcggc
    44581 ggagcaattg ccgaccgctt tggtgccaaa ccgatgattg ttaccggtat gctgatgcgt
    44641 gccgccggat tcgccacaat gggtatcgcc cacgaaccgt ggctactgtg gttttcatgc
    44701 ctgctttcgg gactcggcgg cacgttgttt gatcctccgc gttcggcgct ggtggtgaaa
    44761 ttaatccgtc cacagcagcg tggtcgtttt ttctcgctgt tgatgatgca ggacagtgcc
    44821 ggtgcggtca ttggcgcatt gttggggagc tggctgttgc aatacgactt tcgcctggtc
    44881 tgcgccacag gggcagtttt gtttgtgctg tgtgcagcgt ttaatgcgtg gttgttaccg
    44941 gcatggaaac tctccaccgt acgcacgccc gttcgcgaag gcatgacccg cgtgatgcgc
    45001 gacaagcgtt ttgtcaccta tgtactgacg ctggcgggtt actacatgct ggctgtacaa
    45061 gtgatgctga tgctgccaat tatggtcaac gacgtggcgg gcgcgccctc tgccgttaaa
    45121 tggatgtatg ccattgaagc gtgtctgtcg ttaacgttgc tctaccctat cgcccgctgg
    45181 agtgaaaagc attttcgtct ggaacaccgg ttgatggctg ggctattgat aatgtcatta
    45241 agcatgatgc cggtgggcat ggtcagcggt ctgcaacaac ttttcaccct gatttgtctg
    45301 ttttatatcg ggtcaatcat tgccgagcct gcgcgtgaaa ccttaagtgc ttcgctggcg
    45361 gacgcaagag ctcgcggcag ctatatgggg tttagccgtc tgggtctggc gattggcggc
    45421 gctattggtt acatcggtgg cggctggctg tttgacctgg gcaaatcggc gcaccagcca
    45481 gagcttccgt ggatgatgct gggcattatt ggcatcttca ctttccttgc gctgggttgg
    45541 cagtttagcc agaaacgcgc cgcgcgtcgt ttgctggaac gcgacgcctg atttgatact
    45601 cccccctgac tcctccatac tgaaagtgaa ctgacagcca aatggaggag tcacgtgaag
    45661 ctatacattt acgatcactg cccttactgc ctcaaagccc gcatgatttt cggcctgaaa
    45721 aatatcccca tcgaattaca tgttctgctc aacgacgacg cagaaacacc cacccggatg
    45781 gtcggtcaaa aacaggttcc cattctgcaa aaagatgaca gccgctatat gccagaaagc
    45841 atggacatcg ttcactatgt cgataaactc gacggcaaac cgttactgac cggcaaacgt
    45901 tcccctgcca ttgaagagtg gctgcgcaag gtcaatggct acgccaacaa actgctgttg
    45961 ccgcgttttg ccaaatcggc atttgatgag ttttctactc ctgccgcgcg caaatatttc
    46021 gtcgacaaga aagaggctag cgcgggtaat tttgccgacc tgctggccca ctctgacggt
    46081 ctgattaaga atatcagcga tgatttacgt gcgctggaca aactgatcgt caaaccgaac
    46141 gccgtgaatg gcgaactttc ggaagatgat attcagctat ttccgctcct gcgtaatctg
    46201 acgctggtag ccggaatcaa ctggccaagc cgcgttgctg attaccgcga taatatggcg
    46261 aaacagacac aaatcaattt gttatcatca atggcgattt aatcctgacc cgccgcggca
    46321 gcctgtattc gcgggttgcc gccgttttca gttttttctg ctggcgcgtt tatcgctggt
    46381 gacacatgct gcgttgtgtc aggatattga cgcagatttc atgtcgagga ctaccatgaa
    46441 caagttttta tttgccgctg cattgatcgt cagtggcctg ctcgttggct gtaatcaact
    46501 cacccaatac accatcaccg aacaagaaat taaccagtcg cttgcgaaac ataataattt
    46561 ctcaaaagat atcggtttac ccggcgtggc tgacgcccat attgttctga caaacctgac
    46621 cagccaaatt ggtcgcgaag agccgaataa ggttacccta accggagacg ccaatctgga
    46681 catgaactcc ctgttcggta gccagaaagc gaccatgaaa ctgaagctga aagcgctgcc
    46741 cgtgtttgat aaagaaaaag gcgctatctt cctgaaagag atggaagtgg tcgatgcgac
    46801 ggtacaaccg gaaaaaatgc aaacggtgat gcaaacgttg cttccctatt taaaccaggc
    46861 attacgcaat tactttaacc agcaacctgc ttacgtcctg cgcgaagatg gcagccaggg
    46921 cgaagcaatg gcgaagaaac tggcgaaagg cattgaagtg aagccaggcg aaattgtcat
    46981 tccgtttact gattaatcac gagggcgcat tcgcgccctt tatttttcgt gcaaaggaaa
    47041 acgtttccgc ttatcctttg tgtccggcaa aaacatccct tcagccggag catagagatt
    47101 aatgactgca ccatcccagg tattaaagat ccgccgccca gacgactggc accttcacct
    47161 ccgcgatggc gacatgttaa aaactgtcgt gccgtatacc agcgaaattt atggacgggc
    47221 tatcgtaatg cccaatctgg ctccgcccgt gaccaccgtt gaggctgccg tggcttatcg
    47281 ccagcgtatt cttgacgccg tacctgccgg gcacgatttc accccgctga tgacctgtta
    47341 tttgacagat tcactagacc ctaatgagct tgagcgtgga tttaacgaag gcgtgttcac
    47401 cgctgccaaa ctttacccgg caaacgcaac cactaactcc agtcacggcg tgacgtcagt
    47461 tgacgcaatc atgccggtac ttgagcgcat ggaaaaaatc ggtatgccgt tactggtgca
    47521 tggtgaagtg acacatgcag atatcgacat ttttgatcgt gaagcgcgct ttattgaaag
    47581 cgtgatggaa ccgctacgcc agcgtctgac tgcgctgaaa gtcgtttttg agcacatcac
    47641 caccaaagac gctgccgact atgtccgtga cggaaatgaa cggctggctg ccaccatcac
    47701 tccgcagcat ctgatgttta accgcaacca tatgctggtt ggtggcgtgc gtccgcacct
    47761 gtattgtcta cccatcctca aacgcaatat ccaccaacag gcattgcgtg aactggtcgc
    47821 cagcggtttt aatcgcgtat tcctcggtac ggattctgcg ccgcatgcac gtcatcgcaa
    47881 agagagcagc tgcggctgcg cgggctgctt caacgcccca accgcgctgg gcagttacgc
    47941 taccgtcttt gaagagatga atgctctgca gcactttgaa gcattctgtt ctgtaaacgg
    48001 cccacagttc tatggcttgc cggtcaacga cacattcatc gaactggtcc gcaaagagca
    48061 acaggttgct gaaagcatcg cattgactga tgacacgctg gtgccattcc tcgccgggga
    48121 aacggtacgc tggtccgtta aacaataaaa atcgtagctt cctgttgtca ttaggttatt
    48181 ttaactgtat aaataaccag tatattcaac agggggctat tatgcgaatt gaagtcacca
    48241 tagcgaaaac ttctccattg ccagctgggg ctattgacgc cctggctggc gaactttccc
    48301 gccgtattca gtatgcgttt cctgataatg aaggccacgt atcggtacgt tatgccgcag
    48361 cgaataattt atcggttatt ggcgcgacaa aagaagataa acagcgcatt agcgaaattc
    48421 tccaggaaac gtgggaaagc gccgatgact ggtttgtcag cgaataatat gcagtgattt
    48481 ttgtttttgc cgggtcgccc cggctttttt attttacttg cgaattgtga gtacaaaatt
    48541 tctacacttc ctcaaaataa ttgcacaata tgctaaaaaa atatgaatga catgctgttt
    48601 tattgttcat ataaatctta aaagcaaaaa tttatctgtt ccccccttta aaaattcggt
    48661 gaataccctt acttattggt atactgaagt tgcttcagat aaaaacgcat tgaacctcga
    48721 ataacgttgt ctagtaacac gaattagggg gccatgatgg aaaaaaataa tgaagtcatt
    48781 cagactcatc cgctcgtagg gtgggacatc agcaccgttg atagctatga tgcgctgatg
    48841 ttgcgtttgc actaccagac cccaaataag tccgagcagg aagggactga agttggtcag
    48901 acgctctggt taaccactga tgttgccaga caatttattt cgatattaga agcaggaatc
    48961 gccaaaattg aatccggtga tttcccggta aacgagtatc ggcgtcatta acatgacatg
    49021 accatccacc caaaaggcac ctcaccggtg cctttaccat ttctgggttg tatatctgca
    49081 ttcagttaat tactctgctt aaaccatgag agcagagaga aaaaaatgaa atacgatctc
    49141 atcattattg gcagcggttc cgtaggcgct gccgccgggt attatgcaac ccgtgccggt
    49201 ttaaacgtgc taatgaccga cgcccatatg ccaccgcatc aacacggcag ccaccacggc
    49261 gatacgcgat taattcgcca tgcttatggt gaaggcgaaa agtatgtccc gctggttctc
    49321 cgcgcgcaaa cgctgtggga tgaactctcc cgccacaacg aagaagatcc catttttgta
    49381 cgctctggcg tcattaacct tggcccggct gattccacat ttctcgccaa cgtcgcccac
    49441 agcgccgaac aatggcaact caacgttgaa aagctcgatg cgcaagggat tatggcccgc
    49501 tggccagaaa tacgcgtccc ggacaactac atcggcttat ttgagactga ttccggcttt
    49561 ttacgcagcg aactggcgat taaaacctgg atccaactgg cgaaggaagc gggctgtgcg
    49621 cagctgttca actgcccggt caccgcaatt cgtcatgacg atgatggcgt aactattgaa
    49681 acggctgacg gtgagtatca ggcgaaaaaa gcgattgtct gcgcgggaac atgggtaaaa
    49741 gacctgctcc cggagttgcc tgtccagccc gtacgcaaag tatttgcctg gtatcaggcc
    49801 gatgggcgct atagcgtgaa gaataaattc ccggcgttta ccggtgaact tcccaatggc
    49861 gatcaatatt atggttttcc ggcagaaaac gacgcgttga agattggcaa acataacgga
    49921 ggccaggtta tccattcagc ggatgaacgt gttccgtttg cggaagtggt cagcgatggt
    49981 tcggaagcct tcccgttctt gcgcaatgta ttgccgggta tcggttgctg cctgtacggc
    50041 gccgcctgca cctatgataa ttcgcctgac gaagatttta ttatcgatac cctacccggc
    50101 cacgataata cactgctcat taccggcctg agtgggcacg gttttaaatt tgcgtcagtt
    50161 ttaggggaaa tagctgccga ttttgcgcaa gacaaaaaaa gcgattttga tttgacgcca
    50221 ttcaggcttt cccgcttcca ataatcatca ttacggcctc cagaatgagg ccgtttttac
    50281 aggagtattt aatgcgtcgg ctgttgcact atctcatcaa taatattcgc gagcatctga
    50341 tgctttatct ttttctctgg ggattgttgg caattatgga tctgatctat gtgtattatt
    50401 tttagttttc cgcactgaca taaactgaca aatatttaat ctccctttca tttagcgtga
    50461 aaaattgaat gagattattc ctgcttcaac agcactcacc agacatctct gatgctgaac
    50521 ccactaaact tatgaagatt ctt
//
LOCUS       DAZFLD010000008        49997 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.008, whole genome shotgun sequence.
ACCESSION   DAZFLD010000008 DAZFLD010000000
VERSION     DAZFLD010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 49997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49997
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.008"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(133..273)
                     /gene="yjjY"
                     /locus_tag="ACHKBL_000415"
     CDS             complement(133..273)
                     /gene="yjjY"
                     /locus_tag="ACHKBL_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjjY"
                     /protein_id="HFO5817340.1"
                     /translation="MTKVRNCVLDALSINVNNIISLVVGTFPQDPTVSKTAVILTILT
                     AT"
     gene            369..1085
                     /gene="arcA"
                     /locus_tag="ACHKBL_000416"
     CDS             369..1085
                     /gene="arcA"
                     /locus_tag="ACHKBL_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003621071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HFO5817341.1"
                     /translation="MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILS
                     EYDINLVIMDINLPGKNGLLLARELREQANVALMFLTGRDNEVDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYK
                     LPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDT
                     PEIIATIHGEGYRFCGDLED"
     gene            complement(1144..2496)
                     /gene="creD"
                     /locus_tag="ACHKBL_000417"
     CDS             complement(1144..2496)
                     /gene="creD"
                     /locus_tag="ACHKBL_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HFO5817342.1"
                     /translation="MLKSPLFWKMTTLFGAVLLLLIPIMLIRQVIVERADYRSDVEDA
                     IRQSTSGPQKLVGPLIAIPVTELYTVQEEDKTVERKRSFIHFWLPESLMVDGNQNVEE
                     RKIGIYTGQVWHSDLTLKADFDVSRLSELNAPNITLGKPFIVISVGDARGIGVVKAPE
                     VNGTALTIEPGTGLEQGGQGVHIPLPEGDWRKQNLKLNMALNLSGTGDLSVVPGGRNS
                     EMTLTSNWPHPSFLGDFLPAKREVSESGFQAHWQSSWFANNLGERFASGNDTGWENFP
                     AFSVAVTTPADQYQLTDRATKYAILLIALTFIAFFVFETLTAQRLHPMQYLLVGLSLV
                     MFYLLLLALSEHTGFTVAWIIASLIGAIMNGIYLQAVLKGWCNSMLFTLALLLLDGVM
                     WGLLNSADSALLLGTSVLVVALAGMMFVTRNIDWYAFSLPKMKASKEVTTDDELRIWK
                     "
     gene            complement(2554..3978)
                     /gene="creC"
                     /locus_tag="ACHKBL_000418"
     CDS             complement(2554..3978)
                     /gene="creC"
                     /locus_tag="ACHKBL_000418"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HFO5817343.1"
                     /translation="MRIGMRLLLGYFLLVAVAAWFVLAIFVKEVKPGVRRATEGTLID
                     TATLLAELARPDLLSGDTTHGQLAQAFNQLQHRPFRANIGGINKVRNEYHVYMTDAQG
                     KVLFDSANKAVGQDYSRWNDVWLTLRGQYGARSTLQNPTDPESSVMYVAAPIMDGSRL
                     IGVLSVGKPNAAMAPVIKRSERRILWASAILLGIALVIGAGMVWWINRSIARLTRYAD
                     SVTDNKPVPLPDLGSSELRKLAQALESMRVKLEGKNYIEQYVYALTHELKSPLAAIRG
                     AAEILREGPPPEVVARFTDNILTQNARMQALVETLLRQARLENRQEVVLTAVDVAALF
                     RRVSEARTVQLAEKNITLHVTPTEVNVAAEPALLEQALGNLLDNAIDFTPESGCITLS
                     AEVEQEHVTLKVLDTGSGIPDYALSRIFERFYSLPRANGQKSSGLGLAFVSEVARLFN
                     GEVTLRNVQEGGVLASLRLHRHFT"
     gene            complement(3978..4667)
                     /gene="creB"
                     /locus_tag="ACHKBL_000419"
     CDS             complement(3978..4667)
                     /gene="creB"
                     /locus_tag="ACHKBL_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HFO5817344.1"
                     /translation="MQRETVWLVEDEQGIADTLVYMLQQEGFAVEVFERGLPVLDKAR
                     QQVPDVMILDVGLPDISGFELCRQLLTLHPALPVLFLTARSEEVDRLLGLEIGADDYV
                     AKPFSPREVCARVRTLLRRVKKFSTPSPVIRIGHFELNEPAAQISWFDTPLTLTRYEF
                     LLLKTLLKSPGRVWSRQQLMDSVWEDAQDTYDRTVDTHIKTLRAKLRAINPDLSPINT
                     HRGMGYSLRGL"
     gene            complement(4680..5153)
                     /gene="creA"
                     /locus_tag="ACHKBL_000420"
     CDS             complement(4680..5153)
                     /gene="creA"
                     /locus_tag="ACHKBL_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CreA"
                     /protein_id="HFO5817345.1"
                     /translation="MKYKHLILSLSLIMLGPLAHAEEIGSVDTVFKMIGPDHKIVVEA
                     FDDPDVKNVTCYVSRAKTGGIKGGLGLAEDTSDAAISCQQVGPIELSDRIKNGKAQGE
                     VVFKKRTSLVFKSLQVVRFYDAKRNALAYLAYSDKVVEGSPKNAISAVPVMPWRQ"
     gene            5364..6233
                     /gene="robA"
                     /locus_tag="ACHKBL_000421"
     CDS             5364..6233
                     /gene="robA"
                     /locus_tag="ACHKBL_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     RobA"
                     /protein_id="HFO5817346.1"
                     /translation="MDQAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKD
                     VTGHAIGAYIRARRLSKSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPAL
                     YRRSPEWSAFGIRPPLRLGEFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMR
                     YQFWHDFLGNAPTIPPVLYGLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVM
                     LQGGEYVMFTYEGLGTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRP
                     INLRCELLIPIRR"
     gene            complement(6230..6877)
                     /gene="gpmB"
                     /locus_tag="ACHKBL_000422"
     CDS             complement(6230..6877)
                     /gene="gpmB"
                     /locus_tag="ACHKBL_000422"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase GpmB"
                     /protein_id="HFO5817347.1"
                     /translation="MLQVYLVRHGETQWNAERRIQGQSDSPLTAKGEQQAMQVATRAK
                     ELGITHIISSDLGRTRRTAEIIAQACGCDIIFDSRLRELNMGVLETRNIDSLTEEEEN
                     WRRQLVNGTVDGRIPEGESMQELSDRVNAALESCRDLPQGSRPLLVSHGIALGCLVST
                     ILGLPAWAERRLRLRNCSISRVDYQESLWLASGWVVETAGDISHLDAPALDELQR"
     gene            6929..7444
                     /gene="yjjX"
                     /locus_tag="ACHKBL_000423"
     CDS             6929..7444
                     /gene="yjjX"
                     /locus_tag="ACHKBL_000423"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/xanthosine triphosphatase"
                     /protein_id="HFO5817348.1"
                     /translation="MHQVVCATTNPAKIQAILQAFHEIFGEGSCHIASVAVESGVPEQ
                     PFGSEETRAGARNRVANARRLHPEADFWVAIEAGIDGDSTFSWVVIENASQRGEARSA
                     TLPLPAVILEKVREGEALGPVMSRYTGIDEIGRKEGAIGVFTAGKLTRASVYHQAVIL
                     ALSPFHNAVYQ"
     gene            complement(7438..7764)
                     /gene="trpR"
                     /locus_tag="ACHKBL_000424"
     CDS             complement(7438..7764)
                     /gene="trpR"
                     /locus_tag="ACHKBL_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HFO5817349.1"
                     /translation="MAQQSPYSAAMAEQRHQEWLRFVDLLKNAYQNDLHLPLLNLMLT
                     PDEREALGTRVRIVEELLRGEMSQRELKNELGAGIATITRGSNSLKAAPVELRQWLEE
                     VLLKDH"
     gene            complement(7854..9791)
                     /gene="sltY"
                     /locus_tag="ACHKBL_000425"
     CDS             complement(7854..9791)
                     /gene="sltY"
                     /locus_tag="ACHKBL_000425"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase"
                     /protein_id="HFO5817350.1"
                     /translation="MEKAKQVTWRLLAAGVCLLTVSSVARADSLDEQRSRYAQIKQAW
                     DNRQMDVVEQMMPGLKDYPLYPYLEYRQITDDLMNQPAVTVTNFVRANPTLPPARTLQ
                     SRFVNELARREDWRGLLAFSPEKPGTTEAQCNYYYAKWNTGQSEEAWQGAKELWLTGK
                     SQPNACDKLFSVWRASGKQDPLAYLERIRLAMKAGNTGLVTVLAGQMPADYQTIASAI
                     ISLANNPNTVLTFARTTGATDFTRQMAAVAFASVARQDAENARLMIPSLAQAQQLNED
                     QIQELRDIVAWRLMGNDVTDEQAKWRDDAIMRSQSTSLIERRVRMALGTGDRRGLNTW
                     LARLPMEAKEKDEWRYWQADLLLERGREAEAKEILHQLMQQRGFYPMVAAQRIGEEYE
                     LKIDKAPQNVDSALTQGPEMARVRELMYWNLDNTARSEWANLVKSKSKTEQAQLARYA
                     FNNQWWELSVQATIAGKLWDHLEERFPLAYNDLFKRYTSGKEIPQSYAMAIARQESAW
                     NPKVKSPVGASGLMQIMPGTATHTVKMFSIPGYSSPGQLLDPETNINIGTSYLQYVYQ
                     QFGNNRIFSSAAYNAGPGRVRTWLGNSAGRIDAVAFVESIPFSETRGYVKNVLAYDAY
                     YRYFMGDKPTLMSATEWGRRY"
     gene            10127..11794
                     /gene="ettA"
                     /locus_tag="ACHKBL_000426"
     CDS             10127..11794
                     /gene="ettA"
                     /locus_tag="ACHKBL_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017145039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HFO5817351.1"
                     /translation="MAQFVYTMHRVGKVVPPKRHILKNISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGIDKDIEGEARPQPDIKIGYLPQEPQLNPEHTVRESIEEAVSEVVNALK
                     RLDEVYALYADPDADFDKLAAEQGRLEEIIQAHDGHNLNVQLERAADALRLPDWDAKI
                     ANLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDFEGTVVAITHD
                     RYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKDQRLAQEASQEAARRKSIEKELEWV
                     RQGTKGRQSKGKARLARFEELNSTEYQKRNETNELFIPPGPRLGDKVLEVSNLRKSYG
                     DRLLIDSLSFSIPKGAIVGIIGPNGAGKSTLFRMISGQEQPDSGTITLGETVKLASVD
                     QFRDSMDNSKTVWEEVSGGLDIMKIGNTEMPSRAYVGRFNFKGVDQGKRVGELSGGER
                     GRLHLAKLLQVGGNMLLLDEPTNDLDIETLRALENALLEFPGCAMVISHDRWFLDRIA
                     THILDYQDEGKVEFFEGNFTEYEEYKKRTLGADALEPKRIKYKRIAK"
     gene            complement(11850..12134)
                     /gene="nadS"
                     /locus_tag="ACHKBL_000427"
     CDS             complement(11850..12134)
                     /gene="nadS"
                     /locus_tag="ACHKBL_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NadS family protein"
                     /protein_id="HFO5817352.1"
                     /translation="MSFFDELKTSLEEAVEIKQGLKEPARVTRYEIADVKAIREQLNV
                     SQSEMAKALGTSVDTIKSWESKRRNPTGLAAKVLATIKENPDFFRELASH"
     gene            complement(12136..12468)
                     /locus_tag="ACHKBL_000428"
     CDS             complement(12136..12468)
                     /locus_tag="ACHKBL_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015873600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO5817353.1"
                     /translation="MGKAIIFIETPMFTRQIKQIATDDELKELQKELIGTPDKGDLIQ
                     QTGGLRKVRMAAGSQGKSGSVRIIYFLATEEIIYLIMAYPKNAKDSLTDTEKAQLKKL
                     TKLLKGEI"
     gene            complement(12560..13792)
                     /gene="nadR"
                     /locus_tag="ACHKBL_000429"
     CDS             complement(12560..13792)
                     /gene="nadR"
                     /locus_tag="ACHKBL_000429"
                     /EC_number="2.7.1.22"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418807.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0050262 - ribosylnicotinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
                     /protein_id="HFO5817354.1"
                     /translation="MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPS
                     AQKLEALHRFLGLEFPRQKKTIGVVFGKFYPLHTGHIYLIQRACSQVDELHIIMGFDD
                     TRDRALFEDSAMSQQPTVPDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNG
                     IKKFMAEKGIQPDLIYTSEEADAPQYMEHLGIETVLVDPKRTFMSISGAQIRENPFRY
                     WEYIPTEVKPFFVRTVAILGGESSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDE
                     IALQYSDYDKIALGHAQYIDFAVKYANKVAFIDTDFVTTQAFCKKYEGREHPFVQALI
                     DEYRFDLVILLENNTPWVADGLRSLGSSVDRKEFQNLLVEMLEENNIEFVRVEEDDYD
                     SRFLRCVELVREMMGEQR"
     gene            complement(13813..15195)
                     /gene="radA"
                     /locus_tag="ACHKBL_000430"
     CDS             complement(13813..15195)
                     /gene="radA"
                     /locus_tag="ACHKBL_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HFO5817355.1"
                     /translation="MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPT
                     VARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGG
                     NPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
                     QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMV
                     GHVTKDGSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQG
                     LREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHSMMANPRRVAVGLE
                     QNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQ
                     DLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKL
                     SDALSVFDDL"
     gene            complement(15244..16212)
                     /gene="serB"
                     /locus_tag="ACHKBL_000431"
     CDS             complement(15244..16212)
                     /gene="serB"
                     /locus_tag="ACHKBL_000431"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HFO5817356.1"
                     /translation="MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAGRSGWLLYG
                     RGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLAGSLTARATRLAHEAQLDVAPLG
                     KIPHLRTPGLLVMDMDSTAIQIECIDEIAKLAGTGERVAEVTERAMRGELDFTASLRS
                     RVATLKGADANILQQVRENLPLMPGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKL
                     RLTAVVANELEIMDGKFTGNVIGDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGAND
                     LPMIKAAGLGIAYHAKPKVNEKTEVTIRHADLMGVFCILSGSLNQK"
     gene            16318..16962
                     /gene="ytjB"
                     /locus_tag="ACHKBL_000432"
     CDS             16318..16962
                     /gene="ytjB"
                     /locus_tag="ACHKBL_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
                     /protein_id="HFO5817357.1"
                     /translation="MARTKLKFRLHRAVIVLFCLALLVALMQGASWFSQNHQRQRNPQ
                     LEELARTLARQVTLNVAPLMRTDSPDEKRIQAILDQLTDESRILDAGVYDEQGDLIAR
                     SGESVDVRDRLALDGKKAGGYFNQQIVEPIAGKNGPLGYLRLTLDTHTLATEAQQVDN
                     TTNILRLMLLLSLAIGVVLTRTLLQGKRTRWQQSPFLLTASKPVPEEEESEKKE"
     gene            16990..18006
                     /gene="lplA"
                     /locus_tag="ACHKBL_000433"
     CDS             16990..18006
                     /gene="lplA"
                     /locus_tag="ACHKBL_000433"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase LplA"
                     /protein_id="HFO5817358.1"
                     /translation="MSTLRLLISDSYDPWFNLAVEECIFRQMPATQRVLFLWRNADTV
                     VIGRAQNPWKECNTRRMEEDNVRLARRSSGGGAVFHDLGNTCFTFMAGKPEYDKTIST
                     SIVLNALNALGVSAEASGRNDLVVKTAEGDRKVSGSAYRETKDRGFHHGTLLLNSDLS
                     RLANYLNPDKKKLAAKGITSVRSRVTNLTELLPGITHEQVCEAITEAFFAHYGERVEA
                     EIISPDKTPDLPNFAETFARQSSWEWNFGQAPAFSHLLDERFTWGGVELHFDVEKGHI
                     TRAQVFTDSLNPAPLEALAGRLQGCLYRADMLQQECEALLVDFPEQEKELRELSAWMA
                     GAVR"
     gene            18038..18301
                     /locus_tag="ACHKBL_000434"
     CDS             18038..18301
                     /locus_tag="ACHKBL_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO5817359.1"
                     /translation="MIDLENQEREIINLMFSQGISWLTAVRIRHKLSLAEVSKMLGIS
                     INSLKQIEKTERLSSNIKSKMAGIYGCPPELLICPSWMTAEHK"
     gene            complement(18462..19181)
                     /gene="deoD"
                     /locus_tag="ACHKBL_000435"
     CDS             complement(18462..19181)
                     /gene="deoD"
                     /locus_tag="ACHKBL_000435"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFO5817360.1"
                     /translation="MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVR
                     GMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLR
                     DVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGVDARVGNLFSADLF
                     YSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAER
                     QTTFNDMIKIALESVLLGDKE"
     gene            complement(19238..20461)
                     /gene="deoB"
                     /locus_tag="ACHKBL_000436"
     CDS             complement(19238..20461)
                     /gene="deoB"
                     /locus_tag="ACHKBL_000436"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFO5817361.1"
                     /translation="MKRAFIMVLDSFGIGATEDAERFGDVGADTLGHIAEACAKGEAD
                     HGRKGPLNLPNLTRLGLAKAHEGSTGFIPAGMDGNAEVIGAYAWAHEMSSGKDTPSGH
                     WEIAGVPVLFEWGYFSDHENSFPQELLDKLVERANLPGYLGNCHSSGTVILDQLGEEH
                     MKTGKPIFYTSADSVFQIACHEETFGLDKLYELCEIAREELTNGGYNIGRVIARPFIG
                     DKAGNFQRTGNRHDLAVEPPAPTVLQKLVDEKHGQVVSVGKIADIYANCGITKKVKAT
                     GLDALFDATIKEMKEAGDNTIVFTNFVDFDSSWGHRRDVAGYAAGLELFDRRLPELMS
                     LLRDDDILILTADHGCDPTWTGTDHTREHIPVLVYGPKVKPGSLGHRETFADIGQTLA
                     KYFGTSDMEYGKAMF"
     gene            complement(20513..21835)
                     /gene="deoA"
                     /locus_tag="ACHKBL_000437"
     CDS             complement(20513..21835)
                     /gene="deoA"
                     /locus_tag="ACHKBL_000437"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009032 - thymidine phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HFO5817362.1"
                     /translation="MFLAQEIIRKKRDGHALSDEEIRFFINGIRDNTISEGQIAALAM
                     TIFFHDMTMPERVSLTMAMRDSGTVLDWKSLHLNGPIVDKHSTGGVGDVTSLMLGPMV
                     AACGGYIPMISGRGLGHTGGTLDKLESIPGFDIFPDDNRFREIIKDVGVAIIGQTSSL
                     APADKRFYATRDITATVDSIPLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSE
                     ALAEAIVGVANGAGVRTTALLTDMNQVLASSAGNAVEVREAVQFLTGEYRNPRLFDVT
                     MALCVEMLISGKLAKNDAEARAKLQAVLDNGKAAEVFGRMVAAQKGPTDFVENYAKYL
                     PTAMLTKAVYADTEGFVSEMDTRALGMAVVAMGGGRRQASDTIDYSVGFTDMARLGDQ
                     VDGQRPLAVIHAKDENSWQEAAKAVKAAIKLADKAPESTPTVYRRISE"
     gene            complement(21913..22692)
                     /gene="deoC"
                     /locus_tag="ACHKBL_000438"
     CDS             complement(21913..22692)
                     /gene="deoC"
                     /locus_tag="ACHKBL_000438"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HFO5817363.1"
                     /translation="MTDLKASSLRALKLMDLTTLNDDDTDEKVIALCHQAKTPVGNTA
                     AICIYPRFIPIARKTLKEQGTPEIRIATVTNFPHGNDDIEIALAETRAAIAYGADEVD
                     VVFPYRALMAGNEQVGFDLVKACKEACAAANVLLKVIIETGELKDEALIRKASEISIK
                     AGADFIKTSTGKVAVNATPESARIMMEVIRDMGVEKTVGFKPAGGVRTAEDAQKYLAI
                     ADELFGADWADARHYRFGASSLLASLLKALGHGDGKSASSY"
     gene            22950..24500
                     /gene="yjjI"
                     /locus_tag="ACHKBL_000439"
     CDS             22950..24500
                     /gene="yjjI"
                     /locus_tag="ACHKBL_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
                     /protein_id="HFO5817364.1"
                     /translation="MPTSHENALQQRCQQIVTSPVLSPEQKRHFLALEAENNLPYPQL
                     PAEARRALDEGVICDMFEGHAPYKPRYVLPDYARFLANGSEWLELEGAKDLDDALSLL
                     TILYHHVPSVTSMPVYLGQLDALLQPYVRILTQDEIDIRIKRFWRYLDRTLPDAFMHA
                     NIGPSDSPITRAILRADAELKQVSPNLTFIYDPEITPDDLLLEVAKNICECSKPHIAN
                     GPVHDKIFTKGGYGIVSCYNSLPLAGGGSTLVRLNLKAIAERSESLDDFFTRTLPHYC
                     QQQIAIIDARCEFLYQQSHFFENSFLVKEGLINPERFVPMFGMYGLAEAVNLLCEKEG
                     IAARYGKEAAANEVGYRISAQLAEFVANTPVKYGWQKRAMLHAQSGISSDIGTTPGAR
                     LPYGDEPDPITHLQTVAPHHAYYYSGISDILTLDETIKRNPQALVQLCLGAFKAGMRE
                     FTANVSGNDLVRVTGYMVRLSDLEKYRAEGSRTNTTWLGEEAARNTRILERQPRVISH
                     EQQMRFSQ"
     gene            24472..25335
                     /gene="yjjW"
                     /locus_tag="ACHKBL_000440"
     CDS             24472..25335
                     /gene="yjjW"
                     /locus_tag="ACHKBL_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HFO5817365.1"
                     /translation="MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTM
                     GRCNDCGECVPQCPHQALQIVDGKVVWNAVVCEQCDTCLKMCPQHATPMAQSMSVDEV
                     LSHIRKAVLFIEGITVSGGEATTQLPFVVALFTAIKNDPQLRHLTCLVDSNGMLSETG
                     WEKLLPVCDGAMLDLKAWGSECHQQLTGRDNQQIKRSICLLAERGKLAELRLLVIPGQ
                     VDYLQHIEELAALIKGLGDVPVRLNAFHAHGVYGEAQSWPSATPEDVEQLADALWERG
                     VSRLIFPALYL"
     gene            complement(25553..26332)
                     /gene="yjjV"
                     /locus_tag="ACHKBL_000441"
     CDS             complement(25553..26332)
                     /gene="yjjV"
                     /locus_tag="ACHKBL_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026291.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFO5817366.1"
                     /translation="MICRFIDTHCHFDFPPFSGDEEASLQRAAQAGVGKIIVPATEAE
                     NFARVQALAEKYQPLYAALGLHPGMLKKHSDVSLDQLQQALERRPAKVVAVGEIGLDL
                     FGDDPQFERQQWLLDEQLKLAKRYDLPVILHSRRTHDKLAMHLKRHDLPRTGVVHGFS
                     GSLQQAERFVLLGYKIGVGGTITYPRASKTRDVIAKLPLASLLLETDAPDMPLNGFQG
                     QPNRPEQAARVFDVLCELRPEPEDEIAEVLLNNTYTLFNVP"
     gene            complement(26329..27402)
                     /gene="yjjU"
                     /locus_tag="ACHKBL_000442"
     CDS             complement(26329..27402)
                     /gene="yjjU"
                     /locus_tag="ACHKBL_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HFO5817367.1"
                     /translation="MGQRIPVTLGNIAPLSLRPFQPGRIALVCEGGGQRGIFTAGVLD
                     EFMRAQFNPFDLYLGTSAGAQNLSAYICNQPGYARKVIMRYTTKREFFDPLRFVRGGN
                     LIDLDWLVEATASQMPLQMDTAARLFDSGKSFYMCACRQDDYAPNYFLPTKQNWLDVI
                     RASSAIPGFYRSGVSLEGINYLDGGISDAIPVKEAARQGAKTLVVIRTVPSQMYYTPQ
                     WFKRMERWLGDSSLQPLVNLVQHHETSYRDIQQFIEKPPGQLRIFEIYPPKPLHSIAL
                     GSRIPALREDYKLGRLCGRYFLATVGKLLTEKAPLTRHLVPVVTPESIVIPPAPVAND
                     TLVAEVIDAPQANDPTFNNEDLA"
     gene            complement(27524..27685)
                     /gene="ytjA"
                     /locus_tag="ACHKBL_000443"
     CDS             complement(27524..27685)
                     /gene="ytjA"
                     /locus_tag="ACHKBL_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HFO5817368.1"
                     /translation="MFRWGIIFLVIALIAAALGFGGLAGTAAGAAKIVFVVGIILFLV
                     SLFMGRKRP"
     gene            complement(27812..28417)
                     /gene="osmY"
                     /locus_tag="ACHKBL_000444"
     CDS             complement(27812..28417)
                     /gene="osmY"
                     /locus_tag="ACHKBL_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone OsmY"
                     /protein_id="HFO5817369.1"
                     /translation="MTMTRLKISKTLLAVMLTSAVATGSAYAENNAQTTNESAGQKVD
                     SSMNKVGNFMDDSAITAKVKAALVDHDNIKSTDISVKTDQKVVTLSGFVESQAQAEEA
                     VKVAKGVEGVTSVSDKLHVRDAKEGSVKGYAGDTATTSEIKAKLLADDIVPSRHVKVE
                     TTDGVVQLSGTVDSQAQSDRAESIAKAVDGVKSVKNDLKTK"
     gene            complement(28810..30399)
                     /gene="prfC"
                     /locus_tag="ACHKBL_000445"
     CDS             complement(28810..30399)
                     /gene="prfC"
                     /locus_tag="ACHKBL_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HFO5817370.1"
                     /translation="MTLSPYLQEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQSAG
                     TVKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRT
                     LTAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVEN
                     ELKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAA
                     VGEDLAQQLRDELELVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLDGLVEWA
                     PAPMPRQTDTRTVEASEDKFTGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQV
                     RTAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPN
                     FAPELFRRIRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVV
                     ARLKSEYNVEAVYESVNVATARWVECADAKKFEEFKRKNESQLALDGGDNLAYIATSM
                     VNLRLAQERYPDVQFHQTREH"
     gene            complement(30490..31167)
                     /gene="yjjG"
                     /locus_tag="ACHKBL_000446"
     CDS             complement(30490..31167)
                     /gene="yjjG"
                     /locus_tag="ACHKBL_000446"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HFO5817371.1"
                     /translation="MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQA
                     VNKPLWVDYQNGAITSLQLQHGRFESWAERLNVEPGKLNEAFINAMAEICTPLPGAVS
                     LLNAIRGNAKIGIITNGFSALQQVRLERTGLRDYFDLLVISEEVGVAKPNKKIFDYAL
                     EQAGNPDRSRVLMVGDTAESDILGGINAGLATCWLNAHNREQPEGIAPTWTVSSLHEL
                     EQLLCKH"
     gene            complement(31182..31628)
                     /gene="rimI"
                     /locus_tag="ACHKBL_000447"
     CDS             complement(31182..31628)
                     /gene="rimI"
                     /locus_tag="ACHKBL_000447"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006474 - N-terminal protein amino acid
                     acetylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFO5817372.1"
                     /translation="MNTISSLETTDLPAAYHIEQRAHAFPWSEKTFASNQGERYLNFQ
                     LTQNGKMAAFAITQVVLDEATLFNIAVDPDYQRQGLGRALLEHLIDELEKRGVATLWL
                     EVRASNAAAIALYESLGFNEATIRRNYYPTTDGREDAIIMALPISM"
     gene            complement(31597..32010)
                     /gene="holD"
                     /locus_tag="ACHKBL_000448"
     CDS             complement(31597..32010)
                     /gene="holD"
                     /locus_tag="ACHKBL_000448"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HFO5817373.1"
                     /translation="MTSRRDWQLQQLGITQWSLRRPGALQGEIAIAIPAHVRLVMVAN
                     DLPALTDPIVSDVLRALTVSPDQVLQLTPEKIAMLPQGSRCNSWRLGTDEPLSLEGAQ
                     VASPALTELRANPTARAALWQQICTYEHDFFPRND"
     gene            32113..33144
                     /gene="rsmC"
                     /locus_tag="ACHKBL_000449"
     CDS             32113..33144
                     /gene="rsmC"
                     /locus_tag="ACHKBL_000449"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HFO5817374.1"
                     /translation="MSAFTPASEVLLRHSDDFEQSRILFAGDLQDDLPARLDTAASRA
                     HTQQFHHWQVLSRQMGDNARFSLVATADDVADCDTLIYYWPKNKPEAQFQLMNLLSLL
                     PVGTDIFVVGENRSGVRSAEQMLSDYAPLNKMDSARRCGLYFGRLEKQPVFDADKFWG
                     EYSVDGLTVKTLPGVFSRDGLDVGSQLLLSTLTPHTKGKVLDVGCGAGVLSVAFARHS
                     PKIRLTLCDVSAPAVEASRATLAANGVEGEVFASNVFSEVKGRFDMIISNPPFHDGMQ
                     TSLDAAQTLIRGAVRHLNSGGELRIVANAFLPYPDVLDETFGFHEVIAQTGRFKVYRA
                     IMTRQAKKG"
     gene            33344..33430
                     /locus_tag="ACHKBL_000450"
     tRNA            33344..33430
                     /locus_tag="ACHKBL_000450"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:33378..33380,aa:Leu,seq:cag)
     gene            complement(33469..33705)
                     /gene="yjjZ"
                     /locus_tag="ACHKBL_000451"
     CDS             complement(33469..33705)
                     /gene="yjjZ"
                     /locus_tag="ACHKBL_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1435 domain-containing protein"
                     /protein_id="HFO5817375.1"
                     /translation="MLQRTLGSGWGVLLPGLLIAGLMYADLSPDQWRIIILMGLVLTP
                     AMLYHKQLRHYVLLPSCLALIAGIMLMIMNLKQG"
     gene            33846..34634
                     /gene="fhuF"
                     /locus_tag="ACHKBL_000452"
     CDS             33846..34634
                     /gene="fhuF"
                     /locus_tag="ACHKBL_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-iron reductase FhuF"
                     /protein_id="HFO5817376.1"
                     /translation="MAYRSAPLYEDVIWRTHLQPQDAGLAQAVRATIAEHREHLLEFI
                     RLDEPAPLRAMTLAQWSSPNTLSSQLAVYSDHIYRNQPLMIRENKPLISLWAQWYIGL
                     MVPPLMLALLTQEKALDVSPEHFHVEFHETGRAACFWVDVCEDKNATLHSPQQRMETL
                     ISQALVPVVQALEATGEINGKLIWSNTGYLINWYLTEMKQLLGEATVESLRHALFFEK
                     MLTNGEDNPLWRTVVLRDGLLVRRTCCQRYRLPDVQQCGDCTLK"
     gene            complement(34671..35348)
                     /gene="bglJ"
                     /locus_tag="ACHKBL_000453"
     CDS             complement(34671..35348)
                     /gene="bglJ"
                     /locus_tag="ACHKBL_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator BglJ"
                     /protein_id="HFO5817377.1"
                     /translation="MEHSRIKKRNVALIEKCVMSSIGIESLFRKFAGNPYKLHTYTSQ
                     ESFQDAMSRISFAAVIFSFSAMRSERREGLSCLTELAIKFPRTRRLVIADDDIEARLI
                     GSLSPSPLDGVLSKASTLEIFHQELFLSLNGVRQATDRLNNQWYINQSRTLSPTEREI
                     LRFMSRGYSMTQIAEQLKRNIKTIRAHKFNVMSKLGVSSDAGLLEAADILLCMRHCEA
                     SNVLHPY"
     gene            complement(35306..36031)
                     /gene="yjjQ"
                     /locus_tag="ACHKBL_000454"
     CDS             complement(35306..36031)
                     /gene="yjjQ"
                     /locus_tag="ACHKBL_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO5817378.1"
                     /translation="MLPGCCKNGIVISKIPVMQAGLKEVMRTHFPEYEIISSASAEDL
                     TLLQLRRSGLVIADLAGESEDPRSVCEHYYSLISQYREIHWVFMVSRSWYSQAVELLM
                     CPTATLLSDVEPIENLVKTVRSGNTHAERISAMLTSPAMTETHDFSYRSVILTLSERK
                     VLRLLGKGWGINQIASLLKKSNKTISAQKNSAMRRLAIHSNAEMYAWINSAQGARELN
                     LPSVYGDAAEWNTAELRREMSHS"
     gene            36650..37420
                     /gene="yjjP"
                     /locus_tag="ACHKBL_000455"
     CDS             36650..37420
                     /gene="yjjP"
                     /locus_tag="ACHKBL_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HFO5817379.1"
                     /translation="MQTEQQRAVTRLCIQCGLFLLQHGAESALVDELSSRLGRALGMD
                     SVESSISSNAIVLTTIKDGQCLTSTRKNHDRGINMHVVTEVQHIVILAEHHLLDYKGV
                     EKRFSQIQPLRYPRWLVALMVGLSCACFCKLNNGGWDGAVITFFASTAAMYIRQLLAQ
                     RHLHPQINFCLTAFAATTISGLLLQLPTFSNTPTIAMAASVLLLVPGFPLINAVADMF
                     KGHINTGLARWAIASLLTLATCVGVVMALTIWGLRGWV"
     gene            37411..37884
                     /gene="yjjB"
                     /locus_tag="ACHKBL_000456"
     CDS             37411..37884
                     /gene="yjjB"
                     /locus_tag="ACHKBL_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HFO5817380.1"
                     /translation="MGVIEFLLALAQDMILAAIPAVGFAMVFNVPVRALRWCALLGSI
                     GHGSRMILMTSGLNIEWSTFMASMLVGTIGIQWSRWYLAHPKVFTVAAVIPMFPGISA
                     YTAMISAVKISQLGYSEPLMITLLTNFLTASSIVGALSIGLSIPGLWLYRKRPRV"
     gene            37992..38531
                     /gene="dnaT"
                     /locus_tag="ACHKBL_000457"
     CDS             37992..38531
                     /gene="dnaT"
                     /locus_tag="ACHKBL_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaT"
                     /protein_id="HFO5817381.1"
                     /translation="MSSRVLTPDVVGIDALVHDHQTVLAKAEGGVVAVFANNAPAFYA
                     VTPARLAELLALEEKLARPGSDVALDDQLYQEPQAAPVAVPMGKFAMYPDWQPDADFI
                     RLAALWGVALREPVTTEELASFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPK
                     RDVNTVSEPDSQIPPGFRG"
     gene            38534..39271
                     /gene="dnaC"
                     /locus_tag="ACHKBL_000458"
     CDS             38534..39271
                     /gene="dnaC"
                     /locus_tag="ACHKBL_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HFO5817382.1"
                     /translation="MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGAIRSAAL
                     ERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEGQMNALSKARQYVEEFDGNIASF
                     IFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDL
                     SNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVM
                     DRMRLGNSLWVIFNWDSYRSRVTGKEY"
     gene            39320..39814
                     /gene="yjjA"
                     /locus_tag="ACHKBL_000459"
     CDS             39320..39814
                     /gene="yjjA"
                     /locus_tag="ACHKBL_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein YjjA"
                     /protein_id="HFO5817383.1"
                     /translation="MMKTVKHLLCCAIAASALISTGVHAASWKDALSSAASELGNQNS
                     TTQEGGWSLASLTNLLSSGNQALSADNMNNAAGILQYCAKQKLASVTDAENIKNQVLE
                     KLGLNSEEQKEDTNYLDGIQGLLKTKDGQQLNLDNIGTTPLAEKVKTKACDLVLKQGL
                     NFIS"
     gene            40068..42359
                     /gene="opgB"
                     /locus_tag="ACHKBL_000460"
     CDS             40068..42359
                     /gene="opgB"
                     /locus_tag="ACHKBL_000460"
                     /EC_number="2.7.8.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol--membrane-oligosaccharide
                     glycerophosphotransferase"
                     /protein_id="HFO5817384.1"
                     /translation="MSELLSFALFLASVLIYAWKAGRNTWWFAATLTVLGLFVVLNIT
                     LFASDYFTGDGINDAVLYTLTNSLTDAGVSKYILPGIGIVLGLTAVFGALGWILRRRR
                     HHPHHFGYSLLALLLALGSVDASPAFRQITELVKSQSRDGDPDFAAYYKEPSKTIPDP
                     KLNLVYIYGESLERTYFDNEAFPDLTPELGALKNEGLDFSHTQQLPGTDYTIAGMVAS
                     QCGIPLFAPFEGNASASVSSFFPQNICLGDILKNSGYQNYFVQGANLRFAGKDVFLKS
                     HGFDHLYGSEELKSVVADPHYRNDWGFYDDTVLDEAWKKFEELSRSGQRFSLFTLTVD
                     THHPDGFISRTCNRKKYDFDGKPNQSFSAVSCSQENIATFINKIKASPWFKDTVIVVS
                     SDHLAMNNTAWKYLNKQDRNNLFFVIRGDKPQQETLAVKRNTMDNGATVLDILGGDNY
                     LGLGRSSLSGQSMSEIFLNIKEKTLAWKPDIIRLWKFPKEMKEFTIDQQKNMIAFSGS
                     HFRLPLLLRVSDKRVEPLPESEYSAPLRFQLADFAPRDNFVWVDRCYKMAQLWAPELA
                     LSTDWCVSQGQLGGQQIVQHVDKTTWKGKTAFKDTVIDMARYKGNVDTLKIVDNDIRY
                     KADSFIFNVAGAPEEVKQFSGISRPESWGRWSNAQLGDEVKIEYKHPLPKKFDLVITA
                     KAYGNNASRPIPVRVGNEEQTLVLGNEVTTTTLHFDNPTDADTLVIVPPEPVSTNEGN
                     ILGHSPRKLGIGMVEIKVVEREG"
     gene            complement(42497..43519)
                     /gene="lgoD"
                     /locus_tag="ACHKBL_000461"
     CDS             complement(42497..43519)
                     /gene="lgoD"
                     /locus_tag="ACHKBL_000461"
                     /EC_number="1.1.1.414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-galactonate oxidoreductase"
                     /protein_id="HFO5817385.1"
                     /translation="MSTMNVLICQQPKELVWKQREIPIPGDNEALIKIKSVGICGTDI
                     HAWGGNQPFFSYPRVLGHEICGEIVGLGKNIADLKNGQQVAVIPYVACQQCPACKSGR
                     TNCCEKISVIGVHQDGGFSEYLSVPVANILPADGIDPQAAALIEPFAISAHAVRRAAI
                     APGEQVLVVGAGPIGLGAAAIAKADGALVAVADTSPARREHVATRLELPVLDPSAEDF
                     DAQLRAQFGGSLAQKVIDATGNQHAMNNTVNLIRHGGTVVFVGLFKGELQFSDPEFHK
                     KETTMMGSRNATPEDFAKVGRLMAEGKITADMMLTHRYPFATLAETYERDVINNRELI
                     KGVITF"
     gene            43658..44572
                     /gene="lgoR"
                     /locus_tag="ACHKBL_000462"
     CDS             43658..44572
                     /gene="lgoR"
                     /locus_tag="ACHKBL_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HFO5817386.1"
                     /translation="MSRSQNLRHNVINQVIDDMARGHIPSPLPSQSALAEMYNISRTT
                     VRHILSHLRECGVLTQVGNDYVIARKPDHDDGFACTTASMSEQNKVFEQAFFTMINQR
                     QLRPGETFSELQLARAAGVSPVVVREYLLKFGRYNLIQSEKRGQWSMKQFDQSYAEQL
                     FELREMLETHSLQHFLNLPDHDPRWLQAKTMLERHRLLRDNIGNSFRMFSQLDRDFHS
                     LLLSAADNIFFDQSLEIISVIFHFHYQWDESDLKQRNIIAVDEHMTILSALICRSDLD
                     ATLALRNHLNSAKQSMIRSINENTRYAH"
     gene            44787..46148
                     /gene="lgoT"
                     /locus_tag="ACHKBL_000463"
     CDS             44787..46148
                     /gene="lgoT"
                     /locus_tag="ACHKBL_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO5817387.1"
                     /translation="MEKENITLDPRSSFTPSSSADIPVPPDGLVQRSTRIKRIQTTAM
                     LLLFFAAVINYLDRSSLSVANLTIREELGLSATEIGALLSVFSLAYGIAQLPCGPLLD
                     RKGPRLMLGLGMFFWSLFQAMSGMVHSFTQFVLVRIGMGIGEAPMNPCGVKVINDWFN
                     IKERGRPMGFFNAASTIGVAVSPPILAAMMLVMGWRGMFITIGVLGIFLAIGWYMLYR
                     NREHVELTAVEQAYLNAGSVNARRDPLSFAEWRSLFRNRTMWGMMIGFSGINYTAWLY
                     LAWLPGYLQTAYNLDLKSTGLMAAIPFLFGAAGMLVNGYVTDWLVKGGMAPIKSRKIC
                     IIAGMFCSAAFTLVVPQATTSMTAVLLIGMALFCIHFAGTSCWGLIHVAVASRMTASV
                     GSIQNFASFICASFAPIITGFIVDTTHSFRLALIICGCVTAAGALAYIFLVRQPINDP
                     RKD"
     gene            complement(46197..47861)
                     /gene="tsr"
                     /locus_tag="ACHKBL_000464"
     CDS             complement(46197..47861)
                     /gene="tsr"
                     /locus_tag="ACHKBL_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004888 - transmembrane signaling receptor
                     activity [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HFO5817388.1"
                     /translation="MLKRIKIVTSLLLVLAVFGLLQLTSGGLFFNALKNDKENFTVLQ
                     TIRQQQSTLNGSWVALLQTRNTLNRAGIRYMMDQNNIGSGSTVAELMQSASISLKQAE
                     KNWADYEALPRDPRQSTAAASEIKRNYDIYHNALAELIQLLGAGKINEFFDQPTQGYQ
                     DGFEKQYVAYMEQNDRLYDIAVSDNNASYSQAMWILVGVMIVVLAVILAVWFGIKASL
                     VAPMNRLIDSIRHIAGGDLVKPIEVDGSNEMGQLAESLRHMQGELMRTVGDVRNGANA
                     IYSGASEIATGNNDLSSRTEQQAASLEETAASMEQLTATVKQNAENARQASHLALSAS
                     ETAQRGGKVVDNVVQTMRDISTSSQKIADIISVIDGIAFQTNILALNAAVEAARAGEQ
                     GRGFAVVAGEVRNLAQRSAQAAREIKSLIEDSVGKVDVGSTLVESAGETMAEIVSAVT
                     RVTDIMGEIASASDEQSRGIDQVGLAVAEMDRVTQQNAALVEESAAAAAALEEQASRL
                     TEAVAVFRIQQQQQQQRETSAVVKTVTPATPRKMAVADSGENWETF"
     gene            48043..48906
                     /locus_tag="ACHKBL_000465"
     CDS             48043..48906
                     /locus_tag="ACHKBL_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFO5817389.1"
                     /translation="MAYSIDIISCITERFMALTATEKRIAQFILDDVSAAVELPIAEL
                     ARLTQTSQASVTRFARALGCKDVRELKVKLAQSLAVGQRFILDVPDLDGVQGIYEAII
                     SVLETHRRSLDMNAISQAVSWLSQARQILALGTGGGSTICSQEIQYRLFRLGLPVVSQ
                     NDALMMRMMCSAVMPQDVVIVLSLGGYTPEIIESAAIARQYGARVIAITPAQTPLAEQ
                     VDLVLPLLVQESDYIFKPTPSRYAMLAMVDVLATELAMANKAQAKDRLRRIKLVLDSH
                     RGGANRQPLGD"
     gene            complement(48916..>49997)
                     /locus_tag="ACHKBL_000466"
     CDS             complement(48916..>49997)
                     /locus_tag="ACHKBL_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001084240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HFO5817390.1"
                     /translation="GWLGAHILGGSMYLAWATGIDLTVAKLIIALAFAIYVIIGGYSA
                     VVWTDTIQALILFFGFILMAILAVVHVGGWSAIEQAMDPKAMSLFAIDKMGVLPALSL
                     ALVIGVGVLATPSYRQRIYSGKDVPSVRRSFVITGVLYLFFSILPAIIGMAAFTMNPN
                     LENSNYAFLFATSFLPAILGLVVLIAGLSATMSSASSDAIAAVAIMMRDVYTMITGRM
                     PPEDKAITYSRWMLTFVIGLALVFALTSNDIISYITKMISMLMSGLFICSILGRFWLR
                     FNWQGALAALVSGMVMSIVVLMNADWLAYWGNPCIPSVLGSLVAGVLVTLVTPASQVS
                     REEALAIITNERENQSVVITKPEEA"
BASE COUNT        12027 a        13448 c        12488 g        12034 t
ORIGIN      
        1 attattaaaa taatgtgacg aaagctagca tttagatacg atgatttcat caaactgtta
       61 acgtgctaca attgaacttg atatatgtca acgaagcgta gttttattgg gtgtccggcc
      121 cctcttagcc tgttatgttg ctgttaaaat ggttaggatg acagccgttt ttgacactgt
      181 cgggtcctga gggaaagtac ccacgaccaa gctaatgatg ttgttgacgt tgatggaaag
      241 tgcatcaaga acgcaattac gtactttagt catgttacgc cgatcatgtt aatttgcagc
      301 atgcatcagg caggtcaggg acttttgtac ttcctgtttc gatttagttg gcaatttagg
      361 tagcaaacat gcagaccccg cacattctta tcgttgaaga cgagttggta acacgcaaca
      421 cgttgaaaag tattttcgaa gcggaaggct atgatgtttt cgaagcgaca gatggcgcgg
      481 aaatgcatca gatcctctct gaatatgaca tcaacctggt gatcatggat atcaatctgc
      541 cgggtaagaa cggtcttctg ttagcgcgtg aactgcgcga gcaggcgaat gttgcgttga
      601 tgttcctgac tggccgtgac aacgaagtcg ataaaattct cggcctcgaa atcggtgcag
      661 atgactacat caccaaaccg ttcaacccgc gtgaactgac tattcgtgca cgcaacctgc
      721 tgtcccgtac catgaatctg ggtactgtca gcgaagaacg tcgtagcgtt gaaagctaca
      781 agttcaatgg ttgggaactg gacatcaaca gccgttcgtt gatcggccct gatggcgagc
      841 agtacaagct gccgcgcagc gagttccgcg ccatgcttca cttctgtgaa aacccaggca
      901 aaattcagtc ccgtgctgaa ctgctgaaga aaatgaccgg ccgtgagctg aaaccgcacg
      961 accgtactgt agacgtgacg atccgccgta ttcgtaaaca tttcgaatct acgccggata
     1021 cgccggaaat catcgccacc attcacggtg aaggttatcg cttctgcggt gatctggaag
     1081 attaatcggc tttaccaccg tcaaaaaaac ggcgcttttt agcgccgttt ttatttttca
     1141 accttatttc cagatacgta attcatcgtc cgttgtaact tctttactgg ctttcatttt
     1201 cggcagtgaa aacgcatacc agtcgatatt acgggtcaca aacatcatgc cggccagcgc
     1261 caccaccagc acactggttc ccaacaacag cgcgctatcg gcagagttga gcagtcccca
     1321 catcacacca tccagcaaca acagcgcgag ggtaaacaac atgctgttgc accaaccttt
     1381 caataccgcc tgcaaataaa taccgttcat tatcgcccca atcagactgg cgattatcca
     1441 tgccacggta aaaccggtat gttcagaaag cgccagcaag agcaaataaa acatcaccaa
     1501 tgaaagcccc accagcaaat attgcattgg gtgtaaacgt tgcgcggtga gcgtttcaaa
     1561 aacaaagaac gctataaaag tcagtgcaat cagcagaatg gcgtacttag tcgcccggtc
     1621 agttaattgg tattgatcgg ctggcgtcgt tactgcgacg ctaaacgccg ggaagttttc
     1681 ccagccggta tcattgcctg aagcaaaacg ctcaccgaga ttattagcaa accagctgct
     1741 ttgccagtgc gcctgaaaac ctgactcgct aacttcccgt ttggctggta gaaaatcacc
     1801 taaaaaactg ggatgcggcc agttgctggt taaggtcatt tcgctattac gcccgccagg
     1861 caccacagaa agatcgccgg taccgcttaa attcagggcc atattcagct tcaggttctg
     1921 cttccgccag tccccttcag gtaaagggat atgcacgccc tgcccgcctt gctctaagcc
     1981 ggtgccgggt tcaatggtca gcgccgttcc gttaacttca ggtgctttca ccacaccaat
     2041 accacgcgca tccccgacgc taatcacaat aaatggcttg cctaaggtga tatttggcgc
     2101 gttgagttcg ctaagacgcg aaacatcgaa atcggctttt agcgttaaat cactgtgcca
     2161 gacctgaccg gtataaatcc ctatcttgcg ttcttccacg ttctgattgc catcaaccat
     2221 caatgactca ggtaaccaaa aatggataaa acttcgtttc cgctccacgg ttttatcctc
     2281 ttcctgcacc gtataaagct cggtcacagg aatagcgatg agcggcccaa cgagtttttg
     2341 cggcccgctg gtactttggc gaatcgcatc ttccacatcg ctacggtaat cagcacgttc
     2401 gacaatcacc tgccgaatca gcattatcgg aatcaacaac agcaatactg caccaaacag
     2461 ggtggtcatt ttccagaaca ggggggattt caacatagtc ttctcctttg caatggcagc
     2521 aggatacgaa ggctatgtgg gaagaatttg aagctatgtg aagtgacggt gaagtcgaag
     2581 cgaagccagc acgccacctt cctgcacatt gcgcagcgtg acttcgccgt taaacaaacg
     2641 ggcgacctca ctgacgaacg ccaaccccag accgctgctt ttttgcccat ttgcacgagg
     2701 caaagagtaa aagcgttcaa aaatacgtga aagcgcgtag tcaggaatac cactaccggt
     2761 atccagcacc ttaagcgtga cgtgttcctg ctccacttcg gcgcttagcg tgatgcaacc
     2821 gctctcgggg gtaaaatcga tggcgttatc cagtaaattc cccagagcct gctccagtaa
     2881 cgccggttca gcagcaacgt taacctcggt gggcgtaaca tgtagagtga tgtttttttc
     2941 tgccaactgc acggtgcgcg cttcgctgac gcggcggaat aatgccgcca catcaacagc
     3001 agttagaacg acttcctgac gattctccag tcttgcctgg cgtagtaacg tttctaccaa
     3061 tgcctgcata cgcgcatttt gcgtcagaat gttgtcagta aaacgagcca ccacttccgg
     3121 cggcggacct tcgcgtaaaa tttccgccgc tccacgaatc gccgccagtg ggctttttag
     3181 ctcatgagtt aatgcgtaaa catactgctc aatatagttt ttcccttcca gcttcacgcg
     3241 catactttcc agcgcctgcg cgagtttacg cagctcgcta ctaccgagat cggggagagg
     3301 aacgggctta ttgtcagtga cggaatcagc atagcgagtg agccgggcaa tggagcggtt
     3361 gatccaccaa accatgcctg cgccaatcac cagtgcaatc cccaacaaaa tggcgctggc
     3421 ccataaaatt cgccgctcgc tgcgtttaat gaccggagcc atcgccgcgt tcggtttgcc
     3481 tacgctcaaa acgccaataa gccgcgagcc gtccataatc ggcgcggcaa catacatcac
     3541 cgaactttcg ggatcggtag gattttgcaa cgtgctgcgc gcaccatact gaccacgcaa
     3601 cgttagccag acgtcattcc agcgcgaata atcctgtcca acggctttat ttgccgaatc
     3661 gaacaatact ttgccctgcg catcggtcat atagacatga tattcattgc gcactttgtt
     3721 aatgccaccg atattggcgc gaaacgggcg atgctgtagc tgattaaacg cctgcgccag
     3781 ttgcccatgc gttgtgtccc cagagagcaa atcgggacgc gccagctccg ccagcaacgt
     3841 tgcggtgtcg atcaacgtcc cctccgttgc tcttcgcacg cccggtttaa cttctttgac
     3901 aaaaatggcc agtacgaacc aagctgccac cgccaccagt aaaaaatagc ccagcaacaa
     3961 ccgcatgccg atacgcatta caggcccctc aggctatatc ccatgccgcg atgagtatta
     4021 atcggtgaaa gatcggggtt gatggcgcgc agcttggcac gcagcgtttt aatgtgggta
     4081 tcgacggtgc gatcgtaggt gtcctgcgca tcttcccaca cgctatccat cagttgctgg
     4141 cgggaccaga cgcggcctgg cgacttgagt aacgtcttca gcaataaaaa ctcataccgc
     4201 gtcagcgtta atggcgtgtc aaaccagctg atctgcgccg cgggttcatt caattcaaaa
     4261 tgaccaatac ggatgacggg agacggcgtc gagaacttct tcacccgacg cagtaaggtg
     4321 cgcaccctgg cgcacacttc gcggggtgaa aacggtttag ccacatagtc gtcagcgcca
     4381 atttccagcc caagcaggcg atcgacctct tcacttcggg cggtcaggaa cagtacaggt
     4441 aacgccggat ggagcgtcag taactggcgg cacaattcaa agccactgat atcaggcaaa
     4501 ccaacgtcga gaatcatgac atcgggaacc tgctggcgag ctttatccag caccggcaag
     4561 cctcgctcaa agacctcgac ggcaaaacct tcctgctgca acatgtagac cagcgtgtcg
     4621 gctatccctt gctcatcttc cactaaccag accgtttccc gttgcataaa tcgcctctgt
     4681 tattgccgcc acggcatgac aggaaccgcg ctaatcgcgt ttttcggcga accttctaca
     4741 actttgtcgg agtaagccag ataagcgagc gcgttgcgtt tagcgtcata aaagcgcacg
     4801 acctgtaacg acttaaagac cagggacgtg cgttttttga atactacctc gccctgagct
     4861 ttgccgtttt taatacgatc cgacagttca atcggcccga cttgctgaca agaaatggcc
     4921 gcatcggagg tatcttctgc caggcccaat ccccctttaa taccaccggt tttcgcccga
     4981 ctcacataac aggtgacatt tttcacatcg ggatcgtcaa aggcttccac aacaattttg
     5041 tgatccgggc cgatcatttt aaatacggtg tcgaccgaac caatctcttc tgcatgagcc
     5101 aatggcccca gcattatcag gcttaaagac aggatcaaat gcttgtattt catattgtta
     5161 ccatttttat ctattacacg agcaattagt tcgtcacggt aaataacata ttgattaaga
     5221 acaaaatctc aatactttta tttccgtcaa gccctaaaac atactctact aagaaaaaaa
     5281 cactgaatgc taaaacagca aaaaatgcta ttatccaatt acctgatgtc aggtgctcgt
     5341 tgttgaaagg atgaggatat tttatggatc aggccggcat tattcgcgac cttttaatct
     5401 ggctggaagg tcatctggat cagcccctgt cgctcgacaa tgtagcggcg aaagcaggtt
     5461 attccaagtg gcacttacag agaatgttta aagatgtcac tggccatgct attggcgcgt
     5521 atattcgtgc tcgtcgtttg tcgaaatcgg cggtcgcact acgcctgact gcgcgtccga
     5581 ttctggacat cgcgctgcaa taccgcttcg actctcaaca gacatttacc cgcgcattca
     5641 agaagcagtt tgcccagact cctgcacttt accgccgttc tcctgaatgg agcgcctttg
     5701 gtattcgccc gccgctacgt ctgggtgaat tcactatgcc agagcacaaa tttgtcaccc
     5761 tggaagatac gccgctgatt ggtgttaccc agagctactc ctgttcgctg gagcaaatct
     5821 ctgatttccg ccatgaaatg cgttatcagt tctggcacga ttttctcggc aacgcgccga
     5881 ccattccgcc ggtgctctac ggcctgaatg aaacgcgtcc gagtcaggat aaagacgacg
     5941 aacaagaagt attctatacc accgcgttag cccaggatca ggcagatggc tatgtactga
     6001 cggggcatcc ggtgatgctg cagggcggcg aatatgtgat gtttacctat gaaggtctgg
     6061 gaaccggcgt gcaggagttt atcctgacgg tatacgggac gtgcatgcca atgctcaacc
     6121 tgacgcgccg taaaggtcag gatattgagc gatactaccc ggcagaagat gccaaagcgg
     6181 gagatcgccc aattaatctg cgctgtgaac tactgattcc gatccgtcgt taacgctgca
     6241 gctcatctaa tgcaggggcg tccagatgcg agatgtcccc tgcggtttca acgacccagc
     6301 cagacgccag ccacaggctt tcctgataat ccacgcgcga aatagaacag ttacgcagac
     6361 gtaagcggcg ctctgcccat gctggtaatc cgagaatcgt actcaccaga catcccagtg
     6421 caataccatg gctcaccagc agcggtcggc ttccctgcgg taagtcccgg caagattcca
     6481 gtgccgcatt gacgcgatcg ctgagctcct gcattgactc gccttcagga atacgcccgt
     6541 caacggtgcc attgaccagc tgccgacgcc agttctcttc ttcttcggtg agtgaatcga
     6601 tatttcttgt ttccagtaca cccatattta attcacgcag gcgagaatca aagatgatat
     6661 cacagccgca ggcctgggcg atgatttccg ccgtgcgccg ggtccgtcct aaatcgctac
     6721 tgatgatatg cgtaatgcca agctctttgg cacgggttgc cacctgcatc gcctgttgct
     6781 cacctttggc ggtcagcggg ctgtcagact ggccctgaat acgtcgctcg gcgttccact
     6841 gcgtttcacc gtggcggact aggtatacct gtaacatgct ttttttccgt atactgctgt
     6901 aacgttaatt ttctgagtaa tgctgattat gcaccaagtt gtctgtgcga ccaccaatcc
     6961 cgctaaaatt caggccattc tgcaggcatt tcacgagatc ttcggcgaag gatcctgcca
     7021 tattgcatcc gttgccgtcg agagcggtgt accggaacaa ccctttggca gtgaggaaac
     7081 gcgcgctggc gcacgaaatc gggtagcaaa tgcccgccgt ttacatccag aggctgattt
     7141 ttgggtggcg attgaagcag gcattgatgg cgacagcacc ttcagttggg tggtgattga
     7201 aaacgccagc cagcgcggcg aggcgcgttc ggcaactttg ccgttacctg cggtgattct
     7261 ggaaaaagtg cgtgaaggcg aggcgctcgg tcccgtgatg tcacgttata ccggtattga
     7321 tgagattggt cgtaaggaag gggcgatcgg cgtgtttacc gccgggaaac ttacccgcgc
     7381 cagcgtctat caccaggcgg tgatcctggc gctgagtccg tttcataatg cggtgtatca
     7441 gtgatctttc agcaacacct cttccagcca ctggcgcagc tccacgggcg cggctttcag
     7501 gctgttagat ccacgcgtaa tcgtcgcgat gcccgcgccg agttcatttt ttaactcacg
     7561 ctggctcatt tcgccgcgca acagctcttc gacaatacgc acgcgagtcc ccaacgcttc
     7621 gcgctcatct ggcgtcagca tcaggtttaa caacggtaaa tggagatcgt tttggtaggc
     7681 attcttaagc aggtcgacaa aacgtaacca ctcctggtga cgctgttctg ccatcgctgc
     7741 tgaatagggt gattgttggg ccataatatg tcgccattgt tagcggggga agcaaaatgc
     7801 ctcccccggt tgtactcgct aaagagtacg atagcatatc ataaacgtgc ggatcagtaa
     7861 cgacgtcccc attccgtggc gctcatcaac gtcggtttat cccccatgaa atagcggtag
     7921 taagcgtcat aagccagcac gttcttcaca taaccgcgcg tctcggagaa cggaatactc
     7981 tcgacaaatg ccacagcgtc gatacgcccg gcgctgttgc caagccaggt tcgcacccgt
     8041 ccaggtccgg cgttataagc tgctgaggag aaaatacggt tattgccaaa ctgctgataa
     8101 acatattgca ggtaactggt gccaatgttg atgttcgttt ccggatccag caattgccca
     8161 gggctgctat aaccaggaat agagaacatc ttcaccgtat gggtcgccgt gcccggcata
     8221 atctgcatca ggccgctggc ccctaccggt gatttcactt tcggattcca ggcgctctcc
     8281 tgacgggcaa tcgccatcgc atagctttgc gggatctcct tcccactggt gtaacgtttg
     8341 aaaagatcgt tgtaggccag cgggaatctc tcttccagat gatcccaaag cttcccggcg
     8401 atcgttgcct gaacgctaag ttcccaccat tggttattga aagcataccg agccagttgc
     8461 gcctgctctg tttttgactt gctcttcacc agattggccc actcgctacg cgcggtatta
     8521 tcgagattcc agtacatcaa ctcgcgcacg cgcgccatct ccggcccctg agtcagggcg
     8581 ctgtcaacat tctgcggcgc tttatcaatc ttcagctcat actcttcacc gatgcgttgt
     8641 gctgcaacca tcgggtagaa accacgctgt tgcatgagtt gatgcaaaat ctcttttgct
     8701 tcagcttcac gtccgcgttc cagcagtaaa tccgcctgcc agtaacgcca ttcatctttc
     8761 tctttcgcct ccatcggcaa acgcgccagc caggtattca ggccgcgacg atcgccggtg
     8821 ccaagcgcca ttcgcacgcg ccgttcaata agcgaagtag attgcgaacg cataatggcg
     8881 tcatcgcgcc atttcgcctg ctcatcggtg acatcattgc ccatcaaacg ccaggcgacg
     8941 atatcgcgca gctcctgaat ctgatcttca ttaagctgct gcgcctgggc aagcgacggg
     9001 atcatcagcc gtgcattttc agcatcctgc cgcgccacac tggcaaacgc caccgccgcc
     9061 atttgacggg taaaatcggt cgctccggtt gtacgcgcga aggtcagtac cgtattaggg
     9121 ttgttcgcca gtgaaatgat tgccgaggcg atagtctggt aatcggcagg catctgccct
     9181 gccagcactg ttacgagtcc agtgttaccc gctttcatcg ccagacggat acgctctaaa
     9241 tacgccagcg gatcttgttt acctgacgca cgccagacgc taaataactt gtcacaggcg
     9301 ttaggctggc tcttgccggt tagccacagc tctttcgccc cttgccaggc ttcttcactc
     9361 tgcccggtgt tccatttcgc atagtagtaa ttacattgcg cttcggtagt tccgggcttt
     9421 tccgggctaa aggctaacaa gccacgccag tcttcacgcc gcgccagttc attgacgaaa
     9481 cgagattgca gcgtgcgagc gggaggaagc gtggggttag cgcgaacaaa gttagtgacc
     9541 gtcaccgccg gttgattcat caggtcatcg gtgatctgac ggtattccag gtagggataa
     9601 agcggataat ctttcagtcc aggcatcatt tgttcgacca catccatttg tcgattatcc
     9661 caggcctgct tgatttgggc gtagcggcta cgctgctcat ccagtgagtc ggctcgcgcc
     9721 acgctgctga ccgtcagcag acagacaccg gcagccaaca gccgccaggt aacttgtttg
     9781 gctttttcca caagcgcatc ctctaagtgt aaatacatca atgcagcgtc atgccgcgta
     9841 atagatttat gctaaccagt cattgccgtt tacgccacgt tacggacact tttttacttt
     9901 tatactctaa ataattcgag ttgcaggaag gcgataagtg agtgagtccc caggagctta
     9961 cagaagtaag tgactggggt gagcgagcgt aagcgcagca catgcaactt gaagtatgac
    10021 gagtatactg cgagggtgat cgaccggctg ggattagata aggaaacagg ctacacttcg
    10081 cctttgaaaa ctcatcatca cgataaacat agaggcgaag tccaacgtgg ctcaattcgt
    10141 ttataccatg catcgtgtcg gcaaagttgt tccgccgaaa cgtcatattt tgaaaaacat
    10201 ctctctgagt ttcttccctg gggcaaaaat tggtgtcctg ggtctgaacg gcgcgggtaa
    10261 gtccaccctg ctgcgcatta tggcgggcat tgataaagac atcgaaggcg aagcgcgtcc
    10321 gcaaccggat atcaagattg gttatctgcc gcaggaaccg cagttgaacc cggaacacac
    10381 cgtgcgtgag tccattgaag aagcggtttc tgaagtggtt aacgccctga aacgcctgga
    10441 tgaagtgtat gcactgtacg ccgatccgga tgccgatttt gacaaactgg ccgctgaaca
    10501 gggccgtctg gaagagatca ttcaggctca cgacggtcac aacctgaacg ttcagctgga
    10561 gcgtgcggcg gatgcgctgc gtctgccgga ttgggacgcg aaaatcgcta acctctccgg
    10621 tggtgagcgt cgtcgcgtgg cgttgtgccg cctgctgctg gaaaaaccag acatgctgct
    10681 gctcgacgaa ccgaccaacc acctggatgc cgaatccgtg gcctggctgg aacgcttcct
    10741 gcacgacttc gaaggcaccg ttgtggcgat tacccacgac cgttacttcc tcgataacgt
    10801 tgcgggctgg atcctcgaac tggaccgcgg tgaaggtatt ccgtgggaag gtaactactc
    10861 ctcctggctg gagcagaaag atcagcgtct ggcgcaggaa gcttcacaag aagctgcgcg
    10921 tcgtaagtcg attgagaaag agctggagtg ggtacgtcaa ggtactaaag gccgtcagtc
    10981 gaaaggtaaa gcacgtctgg cgcgctttga agaactgaac agcaccgaat atcagaaacg
    11041 taacgaaacc aacgaactgt ttattccacc tggaccgcgt ctgggcgaca aagtgctgga
    11101 agtcagcaat ctgcgtaaat cctatggcga tcgtctgctg attgactccc tgagtttctc
    11161 gatcccgaaa ggggcgatcg tcgggatcat cggtccgaac ggtgcgggta aatcgacgct
    11221 gttccgtatg atctctggtc aggaacagcc ggacagcggc accatcactt tgggtgaaac
    11281 ggtgaaactg gcatcggttg atcagttccg tgactcaatg gataacagca aaaccgtttg
    11341 ggaagaagtt tccggcgggc tggatatcat gaaaatcggc aacaccgaga tgccaagccg
    11401 cgcctacgtt ggccgcttta actttaaagg ggttgatcag ggtaaacgcg ttggcgaact
    11461 ctccggcggt gagcgcggtc gtctgcatct ggcgaagttg ctgcaagttg gcggcaacat
    11521 gctgctgctc gacgaaccaa ccaacgacct ggatatcgaa accctgcgcg cgctggaaaa
    11581 cgccctgctg gagttcccgg gctgcgcgat ggttatctcg cacgaccgtt ggttcctcga
    11641 ccgtatcgcc acgcacatcc tggattacca ggatgagggt aaagttgagt tcttcgaagg
    11701 taactttacc gagtacgaag agtacaagaa acgcacgctg ggcgcagacg cgctggagcc
    11761 gaagcgtatc aagtacaagc gtattgcgaa gtaatgcgta aaatgccgga tgcggcgcgc
    11821 cagcatcgca tccggtatta gttaaatgat taatgggagg caagttcccg gaaaaagtca
    11881 ggattttctt taattgtggc aagtactttt gcagccaatc ccgtaggatt tcttcgcttc
    11941 gattcccagc ttttaatggt atccacactg gttcccagag cttttgccat ctcactttgc
    12001 gaaacgttga gttgctcacg aatagcttta acatccgcaa tttcgtagcg ggtcacccgt
    12061 gcaggttctt ttaaaccttg tttaatctcg acagcctctt ccagagaggt tttcaactca
    12121 tcaaaaaaac tcattttata tctcgccttt gagcagtttc gtcaacttct ttagttgagc
    12181 tttttccgta tctgtcagac tatctttggc atttttcgga taggccataa tcaaataaat
    12241 aatctcttcc gtcgccagaa agtaaattat tctgacactg ccactttttc cctgtgaacc
    12301 cgcagccatt cggactttac gcaatccacc agtctgctga atgagatcac ctttatcggg
    12361 agttccgatg agttcttttt gtaattcctt taattcatca tcggttgcaa tctgcttgat
    12421 ctgacgcgta aacattggcg tctctataaa tattatcgcc ttacccatta cccctccctg
    12481 gaaggtacat tgtacaccaa aatggagtac taagtacact tttgaatcag taatcccact
    12541 caacatctta taaagatgat tatctttgct cccccatcat ctcccgcacc agctccacgc
    12601 agcgcaggaa acggctgtca taatcgtctt cttcaacccg cacaaattcg atattgttct
    12661 cttccagcat ctccaccagc aagttctgga actctttgcg atccaccgaa ctgccgaggc
    12721 tgcgtaaacc atccgccacc caaggggtgt tgttctccag caagataacc agatcaaaac
    12781 ggtattcatc gatcagcgcc tgcacgaacg gatgctcccg cccttcgtac tttttgcaga
    12841 acgcctgggt agtgacaaaa tcagtgtcga taaacgccac tttattggca tatttcactg
    12901 caaaatcaat gtattgtgcg tgtcccagcg cgattttatc gtagtcggaa tactgcaatg
    12961 cgatctcatc accgccgagg tgtgaaaaga cataatcgcg accatattcc cacgcactgg
    13021 tggtattgaa gatattggca agtttgttta ccagggtgga tttaccgctc gactcgccgc
    13081 caaggatcgc cacggtacgc acaaaaaacg gcttcacttc agtggggata tattcccagt
    13141 agcggaacgg gttttcgcgg atctgcgcac cgctgatact cataaaggtg cgtttcggat
    13201 cgaccagcac cgtttcgatc cccagatgtt ccatatactg tggcgcatcg gcttcttccg
    13261 aggtgtagat caggtccggc tggatcccct tttcagccat aaactttttg atgccgttgc
    13321 tccatacatc ccagccgtgc ggatatggct ccatgccctc ttcattgaaa gcatgaatac
    13381 ggatattttt ctgatattta aaggtttgca ataaccagcg cagacgatcc ggcaccgtgg
    13441 gctgctgcga catggcgctg tcttcgaaca acgcgcggtc gcgggtatcg tcaaagccca
    13501 taatgatatg cagctcgtca acctggctac aggcgcgctg gataaggtag atatgtccgg
    13561 tatgcagcgg gtagaactta ccgaatacga caccaatggt tttcttctgt cggggaaatt
    13621 caagccccaa aaaacggtgc aacgcctcca gcttttgcgc gctggggctt ttaattttgg
    13681 cattcagtaa ctggcttaaa taccctttgg tcataccgct ggcatcagcg acctgctgta
    13741 gcgtgcagcc ctgttgcttg atggcagttt tcaggtaatc aaatgacgac atatctccct
    13801 ccgtatctct cattataagt cgtcgaacac gctaagcgcg tcggagagtt ttttaacgcc
    13861 aaaaatctgc atcccttccg gcgctttttt cggcacgtta gccgccggaa caatcgcccg
    13921 gcgaaaaccg tgtttcgccg cttcagaaat tcgttcctgg ccgctgggca ccgggcggat
    13981 ctcccctgcc agcccgactt caccaaacac caccagatcc tgcggcagcg gtctgtcgcg
    14041 caggctggaa accatcgcca gcagtaacgc taagtcggcg ctggtttccg ttaccttcac
    14101 gccgccgacc acgttaacaa atacatcctg atcggccatt tgcaacccac cgtgacggtg
    14161 cagcaccgcc agcaggattg ccagacggtt ttgctccagc cctactgcga cgcggcgtgg
    14221 gttcgccatc atcgagtgat cgaccagcgc ctggatctcc accaacagtg gacgcgttcc
    14281 ttcccacact accatcactg agctaccgga ggtcacttca tcaccgcggc ttaaaaagat
    14341 ggcggaaggg ttgctaactt cacgcagccc ctgttcggtc atcgcgaaga cgcccagctc
    14401 attcaccgcg ccgaagcggt ttttatggct gcgtaaggtg cgaaaacgag agtcggcatc
    14461 gccatccaga agcaccgaac agtcgataca gtgttccagc actttagggc cagccagcga
    14521 gccatctttg gttacgtgcc caaccatgac aatcgccacg ccgcgcgttt tggcgaagcg
    14581 cgtcagataa gccgccgttt cacgcacctg cgccacgctg ccaggcgatg actgaacatc
    14641 cgccatatgc atcacctgga tggagtcaat taccatcagc ttcggttgct cttcttcggc
    14701 aatcaggcag atctgctcga tgctggtttc cgacagcata ttaagattgt cagtcggcag
    14761 gccaaggcga tgagcgcgca ttgccacctg ttgcagcgac tcttcgccag tgacatacag
    14821 cgttttcatc tgctgcgcca gtttgcacag cgtttgcagc aacagcgtgg atttccccgc
    14881 acctgggtta ccgccaatca gaatggcgct tcccggcacc accccgccgc ctaatacgcg
    14941 gtcgaactct ttaaatccgg tggaaaaacg cggcagctcc tcaaggctga tgtcggaaag
    15001 tttctggact tttgccactc cggcgctacc ggcatagcca ctgagacgct cgttacgcgc
    15061 caccgttggc gacgcagcaa gacgcacctc ggtgatggtg ttccaggcat gacaggcgct
    15121 gcactgcccc tgccagcgcg gataatcggc cccgcattca ttacaaacaa aggcgcgttt
    15181 tggagctttt gccacggtgt acctcgttaa tgctgtgccg cccgcaggat gacgggcagg
    15241 caattacttc tgattcaggc tgccagagag gatgcagaat acccccatca ggtcagcgtg
    15301 acggatggtg acttccgtct tttcattcac ttttggcttg gcatggtagg caatccccag
    15361 ccctgccgct ttgatcatcg gcaggtcatt ggctccatcg ccaatcgcca cggtctgcgc
    15421 cagcgggatt tcatactcct gcgccaggcg ggtcagggtt ttcgctttat attgcgcgtc
    15481 aacgatgtcg ccgatcacat tgccggtaaa tttaccgtcc atgatctcca gttcattggc
    15541 taccactgct gtcaggcgca gcttgtcgcg caggtattca gcaaagaaag taaagccgcc
    15601 ggaggcaatc gccactttcc agcccagcgt ttccagcttg agtaccagct gcgttaagcc
    15661 tggcatcagc ggcagatttt cacgcacctg ttgcagaata ttggcgtcag cgcctttcag
    15721 cgtcgccacg cggctgcgca ggctggcggt aaaatcgagt tcgccgcgca tcgcccgttc
    15781 ggttacctcc gccaccctct cgcccgttcc ggccagtttg gcaatttcat caatacattc
    15841 aatctggatg gcggtggaat ccatgtccat caccagcaaa cccggcgtgc gcaggtgcgg
    15901 gattttcccc agcggcgcga catccagctg cgcttcgtgg gccaggcgcg tagcccgcgc
    15961 ggtgagtgaa cctgccagac gaatcacctg ataatcttcc acgcaccagg cggcaacaat
    16021 caccatcgcc gcgcccagtt tgctctggta ttgggtcaga cgttgtttat ccagcccacg
    16081 accatacagc agccagccgc tacgacctgc gtggtaatcc agtggcatca cttcatcacc
    16141 acttaatgaa agaggcagac ccggccataa agagacatct tcaggcaggt cgcaccaggt
    16201 aatgttaggc attaaggctc ctgtaaaatc gttcgaagca gggaaaataa cgcatgaggc
    16261 taccttgtat ccattgcttc tggcaacatt aagtctcaaa ttttcaaagg gtggaagatg
    16321 gctcgcacaa aactgaaatt ccggctgcat cgggcagtga ttgtcctgtt ctgtcttgcc
    16381 ttgttagtgg cgctgatgca gggagcgtca tggtttagtc aaaaccacca gcgacagcgt
    16441 aatccacagc tggaagaact ggcccgcacc ctggcgcgtc aggtgacgct gaacgttgca
    16501 ccgctgatgc gtaccgactc accggatgaa aaacgcattc aggcgatcct tgatcagtta
    16561 acggatgaaa gccgtatcct cgacgcgggc gtgtatgacg aacaaggcga tcttatcgcc
    16621 cgttctggcg aaagcgtcga cgtgcgcgac cggttggcgc tcgacggcaa aaaagcgggc
    16681 ggctatttta accagcagat tgtcgaacca attgccggga aaaacggacc gctcggctat
    16741 ctgcgcctta cgctcgacac ccatacgctc gccaccgaag cccaacaggt ggataacacc
    16801 actaacattt tacgcctgat gttgctgctc tcactggcaa tcggcgtagt gctgacccgc
    16861 acgctgctac agggtaaacg cacccgctgg cagcaatcgc ccttcctgtt aaccgccagt
    16921 aaaccggtgc cggaagagga agaaagcgag aaaaaagagt gacccattac tacaagaaag
    16981 gaaatcgtta tgtccacatt acgcctgctc atctctgact cttacgaccc gtggtttaac
    17041 ctggcggtag aggagtgtat ttttcgccag atgccggcta cgcagcgcgt tctgtttctc
    17101 tggcgcaatg ccgacacggt agtgattggt cgcgcgcaga acccgtggaa agagtgtaat
    17161 acccggcgga tggaagaaga taacgtccgc ctggcgcggc gcagtagcgg tggcggcgca
    17221 gtgttccacg atctcggcaa tacctgtttt acctttatgg ctggcaagcc ggagtacgat
    17281 aaaaccatct ccacgtcgat tgtgctcaat gcgctgaacg cgctcggcgt cagcgccgaa
    17341 gcttccgggc gtaacgatct ggtggtgaaa accgccgaag gcgatcgcaa agtctcaggc
    17401 tcggcctatc gcgaaaccaa agatcgtggc ttccaccacg gcactttgct gctcaattcc
    17461 gaccttagcc gcctggcaaa ctatctcaat ccggataaaa agaaactggc ggcgaaaggc
    17521 attacctcag tgcgttcccg cgtgaccaat ctgaccgaat tgctaccggg gatcacccat
    17581 gagcaggttt gcgaggccat aaccgaggcc tttttcgccc attatggcga gcgcgtggaa
    17641 gcggaaatca tctccccgga caaaacgcca gacttgccaa acttcgccga aacctttgcc
    17701 cgccagagta gctgggaatg gaacttcggt caggctccgg cattctcgca tctgctggat
    17761 gaacgcttta cctggggcgg cgtggaactg catttcgacg ttgaaaaagg ccatatcacc
    17821 cgcgcccagg tgtttaccga cagtctcaac cccgcgccgc tggaagccct cgccgggcga
    17881 ctgcaaggct gtctgtaccg cgcggatatg ctgcaacagg agtgcgaagc gctgctggtt
    17941 gacttcccgg aacaggaaaa agagctacgg gagttgtcag cgtggatggc aggggcggta
    18001 aggtaactcg ctgattaaaa tatccaggga aggaaatatg atcgatctgg aaaaccagga
    18061 acgggagatt atcaatttaa tgttcagcca gggtatatcc tggctaaccg ccgtgcgtat
    18121 caggcataaa ctatcacttg ctgaagtaag taaaatgtta ggtatctcca ttaattctct
    18181 aaaacagatc gaaaagacgg aacggcttag ttcaaacatc aaaagcaaaa tggcaggaat
    18241 ttatggttgt ccaccagagt tgctgatatg cccttcctgg atgacggctg aacataaatg
    18301 aatagattcg tgaatagata cttgaatgga ttttattttc tatccatatg aaaaataata
    18361 gtaaatctat aaatcagttg ctattgatga ataaatacta aaaagccgga gcagtctccc
    18421 actccggctt cacacgacaa tcgcctttca gcaaaacaca attactcttt atcgcccagc
    18481 agaacggatt ccagcgcgat tttgatcatg tcgttgaagg tggtctgacg ctcagcggca
    18541 gtggtctgct cgtgagtgcg gatgtggtca gacacggtgc agatggtcag ggctttcgcg
    18601 ccaaattctg cagcgacgcc gtagatacca gccgcttcca tttccacgcc gaggatgccg
    18661 tatttttcca tcacgtcgaa catttcgccg tccggagagt agaacaggtc agcggagaac
    18721 aggttaccaa cgcgagcatc aacgcccagt gcttttgccg cgtctaccgc gttacgcacc
    18781 atgtcgaagt cagcgatagc ggcaaagtca tggtctttaa aacggatgcg gttaactttg
    18841 gaatcggtgc aggcacccat accgataacg acgtcacgca gttttacgtg cggcagaaca
    18901 gcaccacagg aacctacgcg gataattttc ttcacgccga aatcggtgat cagttctttg
    18961 gtgtagatgg agcaggacgg gatacccata ccgtgaccca ttacggaaat tttgcggcct
    19021 ttataagtac cggtgaagcc cagcataccg cgaacgttgt tcacttcacg ggcatcttca
    19081 aggaaagttt cagcaatata cttcgcacgc agcgggtcgc ctggcatcaa aactacgtca
    19141 gcaaaatcgc ccatttctgc attaatgtgt ggagtagcca tcgttttatc ctttttgtga
    19201 catattaaag gcggagtcac tgctccgccc aaatccatca gaacatggct ttgccatatt
    19261 ccatatcaga agtaccaaaa tattttgcca gagtctggcc gatatccgcg aaggtttcac
    19321 gatgccccag cgaacctggt ttcactttcg ggccgtaaac cagtaccgga atgtgttcac
    19381 gcgtgtggtc agtaccggtc caggtcgggt cacaaccgtg gtcagcagtg agaatcagaa
    19441 tatcgtcatc gcgcagcaaa gacatcagct ccggcagacg gcggtcgaac agttccagac
    19501 ccgcagcata accggcgacg tcgcgacggt ggccccagga agagtcgaag tcaacgaagt
    19561 tggtgaagac gatggtgtta tcacccgctt ctttcatctc tttgatggtg gcgtcaaaca
    19621 gcgcgtccag gccggtcgct ttcacttttt tggtgatacc gcagttggca tagatgtctg
    19681 caattttacc gaccgaaacc acctggccgt gtttttcatc aaccagtttc tgcagcacgg
    19741 tcggtgctgg cggctcaaca gccaggtcgt gacggttacc ggtacgctgg aagttaccgg
    19801 ctttgtcgcc gataaacgga cgagcgataa cacgaccaat gttgtagccg ccgttggtca
    19861 gctcttcacg ggcgatttcg cacagttcgt agagtttatc cagaccaaag gtttcttcgt
    19921 ggcaagcaat ctggaacacg gagtcagcgg aggtatagaa aatcggcttg ccggttttca
    19981 tgtgctcttc gcccagttga tccagaatga ccgtaccgga agagtggcag ttaccgaggt
    20041 aacccggcag attagcgcgt tcaaccagtt tatccagcag ctcttgcggg aagctgtttt
    20101 cgtgatcgga gaaatatccc cactcaaaca gaaccgggac accggcgatt tcccagtgac
    20161 cagacggggt gtctttaccg gatgacattt cgtgcgccca tgcgtacgcg ccgataactt
    20221 cagcgttgcc atccattccc gccggaatga aaccggtaga accttcgtgt gctttcgcca
    20281 gcccaagacg ggtcagattt ggcagattga gcgggccttt acgaccgtgg tcagcttcgc
    20341 ctttggcaca agcttctgcg atatgaccca gggtgtcagc cccgacgtca ccaaagcgtt
    20401 ctgcatcttc tgtagcgccg atgccgaatg agtccagcac cataataaat gcacgtttca
    20461 tatgttctcc gtactcagtg cttcaaattt aaatgatcag atcagtatac cgttattcgc
    20521 tgatacggcg atagacagtt ggtgtgcttt ccggtgcttt atcggcaagt ttaattgccg
    20581 ctttcaccgc tttcgccgct tcctgccagc tgttttcgtc tttcgcgtgg ataactgcca
    20641 gcggacgctg accgtctacc tggtcgccca gacgcgccat atcagtaaag ccgacgctgt
    20701 aatcgatggt gtcagatgcc tgacggcgtc cgccgcccat tgcaaccact gccatcccca
    20761 gtgcgcgggt atccatttca ctgacaaacc cttcggtatc agcatagact gctttcgtca
    20821 gcatcgctgt cggcagatac ttcgcgtagt tctcaacgaa gtcggtcggg cctttttgtg
    20881 ccgcgaccat acgaccaaag acttctgccg ctttaccgtt gtccagcacc gcctgcaatt
    20941 tcgcgcgcgc ttcggcgtca tttttcgcca gtttgccgga gatcagcatc tccacgcaca
    21001 gtgccatcgt gacatcaaac agacgcgggt tacgatattc acccgtcagg aactgcaccg
    21061 cttcacgaac ttcaaccgcg ttacctgcac tggaggccag tacctgattc atatcggtga
    21121 gcagcgcggt ggtgcgcacg ccagcgccgt tagccacgcc aacaatcgct tcggcaaggg
    21181 cttcagagag ttcgtaggtc ggcataaacg cgccgctacc tactttcacg tccatcacca
    21241 gcgcgtccag accttccgcc agtttcttcg ccagaataga ggcagtgatc agcgggatgg
    21301 agtccacggt tgcggtaata tcacgggtcg cgtagaaacg tttatccgcc ggagccagtg
    21361 agctggtctg gccgataatc gccacgccga cgtctttaat aatttcgcgg aaacggttgt
    21421 catccgggaa aatgtcgaag ccagggatgg attccagttt gtcgagcgta ccgccagtat
    21481 gaccgaggcc gcgaccggag atcatcggaa tatagccgcc gcaggctgcg accatcggcc
    21541 ccaacatcag cgaagtgaca tcgccgacgc cgccggtcga gtgtttatca acaatcgggc
    21601 cattcagatg caggcttttc cagtcgagaa cggttcctga atctcgcata gccatggtca
    21661 gcgagacacg ctcaggcatt gtcatatcgt ggaagaaaat ggtcatcgcg agggcggcaa
    21721 tctgcccttc ggagatagtg ttgtcgcgaa taccgttgat aaagaaacga atttcttcat
    21781 cgctcagcgc atgaccatca cgttttttac gaataatttc ttgtgcgaga aacaaggtaa
    21841 cctcccgtgg aaaagagacg aggtaatcgg ggaaccggat gagcatcgct gcatcatccg
    21901 gcaaatcaag ccttagtagc tgctggcgct cttaccgtcg ccgtggccca gagctttcag
    21961 caggcttgcc agcaggctgg aagcaccaaa gcggtagtga cgcgcatcag cccagtcagc
    22021 accgaacagt tcatcggcga tggcgagata tttctgcgca tcttccgcag tacgcacgcc
    22081 gcccgccggt ttgaaaccaa cggttttttc tacgcccata tcacggatca cttccatcat
    22141 gatgcgcgcg ctttccggcg tcgcgttcac agccacttta ccggtagagg ttttgatgaa
    22201 gtccgcaccc gctttgatgg agatttcaga cgctttacgg atcagcgctt cgtcttttag
    22261 ttcgccggtt tcgatgatca ctttcagcag tacattcgct gccgcgcaag cctctttaca
    22321 ggctttcacc aggtcaaaac caacctgctc gttacccgcc atcagcgcgc ggtacgggaa
    22381 cacaacgtca acttcatcag caccgtaggc gattgccgca cgggtttctg ccagcgcgat
    22441 ttcgatgtcg tcgttaccgt gtgggaagtt ggttaccgta gcgatacgga tttccggggt
    22501 accctgctct ttcagcgttt tgcgagcaat tgggataaag cgaggataga tacagatagc
    22561 ggcggtattg ccgaccggag ttttggcctg atgacacaga gcaattactt tctcgtcggt
    22621 gtcgtcgtca ttcagggtgg tcaggtccat caatttcagt gcacgcaggc tgcttgcttt
    22681 cagatcagtc atttcattct cctggcttgt cgccaataaa attcaccttg cgagtttgtt
    22741 agtattctaa catctactcc gcaacacact tcgatataca tcacaattaa ggaaatctac
    22801 ttacaagttt gcatcactgt aatgcgatct ggttcaaata attcactttc aaatgaatgc
    22861 gtcagtggtg gcaaacgcat caggatcaaa gtgaacatca cgaaacttct tacaatggcg
    22921 catcaccttc ggcataaaag gaaaagaaga tgcccacttc tcatgaaaat gcactgcaac
    22981 aacgttgcca gcaaattgtc accagcccgg tacttagccc ggagcagaag cgccattttc
    23041 tggcactgga agcagaaaac aatctgcctt acccacagct tcctgccgaa gcccgccgcg
    23101 cgctggatga aggtgtaatc tgcgatatgt ttgaaggtca tgcgccgtac aaaccgcgct
    23161 atgtcttacc cgattacgcc cgttttctgg cgaacggttc cgaatggctg gagctggaag
    23221 gcgcgaaaga tcttgatgat gcactctctc tgctgaccat tctttaccac cacgtaccat
    23281 cggtcacatc gatgccggtc tacctggggc aactggatgc gttgttgcaa ccgtatgtta
    23341 gaattctaac acaagacgag atcgatattc gaataaaacg tttctggcgt tacctcgaca
    23401 gaaccctgcc agacgccttt atgcacgcca atatcggccc gtctgattcg cccattaccc
    23461 gcgcgatctt acgtgcagac gcagagttga agcaggtttc accgaacctg acctttatct
    23521 acgatcctga aatcacccct gatgacctac tgctggaagt ggcgaagaac atctgtgaat
    23581 gtagcaaacc gcacatcgcc aacggtccgg tgcatgataa aattttcaca aaagggggct
    23641 acgggattgt gagctgttac aactcactgc cgctggcggg tggtggcagc acgctggtac
    23701 gccttaacct gaaagccatt gccgagcgca gtgaatcgct ggatgacttc tttacacgca
    23761 ctctaccgca ctactgccag cagcagatcg ccatcatcga tgcgcggtgt gaattcctct
    23821 atcaacaatc acacttcttt gagaatagct tcctggtgaa agaagggctg attaaccctg
    23881 aacgttttgt gccaatgttt ggcatgtacg gattggcgga agcagttaac ttgctgtgtg
    23941 agaaagaagg gattgccgcg cgctacggta aagaagccgc cgcaaatgaa gtgggttatc
    24001 gcatcagcgc acaactggcg gagtttgtcg ccaatacccc cgtgaaatat ggctggcaaa
    24061 aacgcgccat gctacacgca cagtcgggga tcagttccga tatcggcacc acgcctggcg
    24121 cacgcttgcc gtatggtgat gagccagatc cgattaccca tctgcaaact gtcgccccgc
    24181 atcatgctta ttattattcc ggcatcagcg acattctgac gctcgacgaa accatcaaac
    24241 gtaatccgca ggcgctggta cagctttgcc tcggtgcctt taaagccgga atgcgtgaat
    24301 ttaccgccaa tgtcagcggt aacgatctgg ttcgcgttac cggttatatg gtgcgtttgt
    24361 cggatttaga aaaatatcgc gccgaaggtt cacgcaccaa caccacctgg ctgggagaag
    24421 aagccgcacg caacactcgt attctggaac gccagccgcg cgtgataagc catgaacagc
    24481 agatgcgctt tagtcagtaa gattatcccc ttctcctgcg ttgacgggcc aggcagtcgt
    24541 ctggctctgt ttttgcaggg ctgcaatctg cgctgcaaaa actgtcacaa tccgtggacg
    24601 atggggcgct gtaatgattg cggagagtgc gtgccgcagt gtccgcatca ggcgttgcag
    24661 attgtcgacg gcaaagtggt gtggaacgct gtggtctgcg agcaatgtga tacctgcctg
    24721 aagatgtgtc cgcaacatgc cacgccaatg gcgcagtcca tgagcgtgga tgaggtgctt
    24781 agccatatcc gcaaagcagt gctgtttatc gaagggataa cggtaagcgg cggcgaagcc
    24841 acgacccagc taccgtttgt ggtggcgctg tttactgcta tcaaaaacga tccgcaactg
    24901 cgccacctca cctgtctggt ggacagtaat ggcatgttga gcgaaaccgg ctgggaaaaa
    24961 ttgcttccgg tgtgcgacgg cgcaatgctc gatctcaaag cgtgggggag cgaatgtcat
    25021 cagcaattaa ccggacgcga taatcagcag atcaagcgca gcatctgttt gctggcagag
    25081 cgcggtaagc tggcggaact gcgtttgctg gtaatccctg gccaggtgga ttatttgcaa
    25141 cacatcgaag aactggcggc gttaatcaag ggacttggcg atgttccggt acgcctgaac
    25201 gcgtttcatg cccacggcgt gtatggcgag gcgcaaagct ggccgagcgc cacgccggag
    25261 gatgtggaac aactggctga tgcgttatgg gagcgcgggg tgagccggtt aatatttccg
    25321 gcgctatatt tgtgacatgc cggatacggc ctacggatcg gcacagacct gtaggcatga
    25381 taagatgcgt caagcgtcgc atcaggcatt gtgcgccagc tgccggatgc ggcgtgaacg
    25441 ccttatccgg cctacggttc ggcacagact tgtaggcatg ataagacgcg tcaagcgtcg
    25501 catcaggcat tgtgcgccaa ctgccggatg cggcgtgaac gccttatccg gcctacggca
    25561 cgttaaacaa cgtatacgta ttatttagca acacttcggc aatctcatct tctggttccg
    25621 ggcgtaactc gcaaagtacg tcaaacaccc gcgcagcctg ttccgggcgg ttaggctgcc
    25681 cctgaaagcc gttgagcggc atatccggcg cgtcggtttc cagcaacaac gatgccagcg
    25741 gtaattttgc gatgacatcg cgggttttac tggcgcgtgg ataggtgata gtaccgccta
    25801 cgccgatttt gtagcccagc agtacaaacc tttcggcctg ttgcaggctg ccggaaaaac
    25861 cgtgaaccac gccagtgcgt ggcaaatcgt ggcgtttaag atgcatcgcc agtttgtcgt
    25921 gtgtgcgccg tgaatgcagg atcaccggca gatcgtagcg tttcgccagt ttcagttgtt
    25981 cgtcgagtaa ccactgctgc ctctcaaatt gcggatcgtc gccgaagaga tccagaccga
    26041 tctcccccac cgccaccacc ttcgccggac gcctttccag cgcctgctgt agctgatcaa
    26101 gagacacatc gctatgtttt ttcaacatac caggatgcaa gcccaatgcg gcatacagcg
    26161 gttgatattt ttccgctaac gcctgcacgc gggcaaaatt ttccgcctct gtcgccggaa
    26221 caatgatctt gcctacgccc gcttgtgccg cgcgttgcag gctggcctct tcatcaccac
    26281 taaaaggcgg gaaatcaaaa tggcagtggg tgtcgataaa acggcaaatc aagccagatc
    26341 ctcattgtta aatgtcgggt cgttcgcctg cggagcgtcg atcacttcgg caaccagcgt
    26401 atcgttggcg actggcgcag gcggaatgac aatcgattcc ggcgtcacca ccggcaccag
    26461 atggcgggta agcggcgctt tttcagtcaa taacttaccg accgttgcca gaaaataacg
    26521 accgcataaa cgcccaagtt tatagtcttc acgcagcgcc ggaatccgac tgccaagcgc
    26581 gatactatgc aacggcttcg gcggataaat ttcgaatatc cgcagctggc ccggtggttt
    26641 ctcaataaat tgctgaatgt cacgatagct ggtttcatga tgctgcacca gattgaccag
    26701 cggctgcagg ctactgtcac ccagccagcg ttccatgcgt ttgaaccact gcggcgtgta
    26761 gtacatttgc gacggcacgg tgcggatgac gaccaaggtt tttgctccct gccttgccgc
    26821 ctctttaacc ggaatcgcat cactgatccc gccatccagg tagttaatgc cttccagcga
    26881 cactccgcta cgataaaagc caggtatcgc actggaggcg cgaatcacat ccagccagtt
    26941 ttgtttggtt ggtaaaaagt aattcggcgc atagtcatcc tgacgacagg cgcacatata
    27001 aaacgatttg ccgctgtcaa acaaccgcgc ggcggtatcc atttgcaacg gcatctggct
    27061 tgcagtggcc tccaccagcc agtcgagatc gataagattt cctccacgga caaagcgcaa
    27121 tggatcgaaa aattcgcgtt ttgtggtata gcgcatgatg actttgcgcg cgtaaccggg
    27181 ctgattgcag atatacgccg agaggttctg cgccccggca gatgtgccga gataaagatc
    27241 gaaaggatta aactgcgcgc gcataaactc atccagcacg ccagccgtga aaattccacg
    27301 ctgtccgccg ccttcgcaca ccagagctat tcgtccaggc tggaacggcc ttagcgacaa
    27361 cggcgcaata ttaccaagcg ttacaggtat tcgctgcccc acctctgcct tcctgttttt
    27421 gttgtgaaag aatgacaaag taacgcaatt tattattcgc taaaaccgca aaagccagtc
    27481 cgctggactg gctttggata aaattttctt aatggtctga aatctagggt cgttttcggc
    27541 ccatgaacaa actcaccagg aacagaataa tcccgacgac aaagacaatt ttagctgcgc
    27601 ctgcagcggt accggccaga ccaccaaacc caagtgcggc ggcgattaac gcgataacca
    27661 gaaatatgat gccccaacga aacataagcg tctcctttac catagttaat gtcaccgctt
    27721 gatatgagcg tgaaaatcac tcaatgcgat atttttagtg tggtgcacat cgcgcctccc
    27781 gacaaagttc gggaggacga attacgacga attacttagt tttcagatca tttttaacgc
    27841 ttttcacacc atctaccgct ttggcgatac tttcagcacg gtcgctctgt gcctgggaat
    27901 cgacggtacc ggagagctga accacgccgt cggtggtttc aactttcaca tgacgggaag
    27961 ggacgatatc gtccgccagc agtttggctt tgatttcact ggtggtggcg gtgtcacccg
    28021 cgtagccctt caccgagcct tctttagcgt cgcgaacgtg cagtttgtcg ctgacagagg
    28081 tcaccccttc aacgcctttc gccactttca ctgcctcttc ggcctgagcc tggctttcaa
    28141 cgaaaccgct cagggtcacg actttttgat cggttttgac agagatatcg gtgctcttga
    28201 tgttgtcatg atccaccagc gccgccttca ctttcgcggt gatggcgctg tcatccatga
    28261 aattaccgac tttattcata gagctatcga ctttttgccc tgcgctttca ttggtagtct
    28321 gtgcgttgtt ttccgcgtag gcagagccgg tcgcgacggc agaggtcaac attacagcca
    28381 gcagagtttt cgaaatcttc agtcttgtca tagtcatcga tttattcctg tatgtttgct
    28441 cgtaatttga gcctggcaac acgaggttgc attgctgaat agggagagac ttcaccctct
    28501 acagaagtca atggccgcca tcacaaaagc gatgggtgat gaataacgac cattacagcc
    28561 tctgaatcag ttattaatat cggtagaatg acaatcgacg gctttagata ctgatatcta
    28621 cgcattgaac ggtatttaac gccgtcagaa atgtcattac tttgttaaat atagatcaca
    28681 attttgaaac cgctcgggat atcagcgaaa acataagcaa aagtgaatgt tttaagaaca
    28741 ttccgtaagc ggctaataag gaagggaaat tgacagggcg cagcggctac cgcgccctgg
    28801 aggcaagaat taatgctcgc gggtctggtg gaactgaacg tctggataac gttcctgcgc
    28861 caggcgcagg ttgaccatgc tggtagcgat gtaagcgagg ttatcgccgc catcaagcgc
    28921 cagttggctt tcgttcttac gcttgaactc ttcgaatttc ttcgcgtccg cacattctac
    28981 ccagcgggca gtggcaacgt tgactgactc atacactgct tcaacgttgt attcgctctt
    29041 cagacgcgct accaccacat caaactgcag cacaccaacc gcaccaacga tcagatcgtt
    29101 gttggagatc ggacggaaca cctgcaccgc gccctcttcg gaaagctgta ccagcccttt
    29161 aagcagctgt ttttgcttca gcggatcttt caggcggata cgacggaaca gttctggcgc
    29221 gaagttcgga ataccggtga acttcatcat ctcaccctgg gtaaaggtgt cgccgatctg
    29281 aatggtgccg tggttgtgca gaccaaggat atcgccagga tacgcttctt caacgtgcga
    29341 acggtcaccc gccataaagg tcagcgcgtc ggagatcacc acatctttcg cagtacgcac
    29401 ctggcgcagc ttcatgcctt tttcatattt accggacacc acacgcataa acgccacgcg
    29461 gtcgcggtgt ttcgggtcca tgttggcctg aattttaaat acgaagccgg taaatttgtc
    29521 ttcgctcgcc tctacggtac gggtatcagt ctgacgcggc atcggcgcag gagcccactc
    29581 caccaggcca tccagcatat gatcaacgcc gaagttaccc agcgcagtac cgaagaatac
    29641 cggagtgatt tcgcccgcaa ggaacagctc tttgtcgaac tcgttagacg cgcctttcac
    29701 cagctccagt tcgtcacgta gctgctgtgc cagatcttca ccaaccgcag cgtcgagatc
    29761 cgggttattc agccctttaa caatgcggac ttcctgaatc gtgtggcctt taccgctctg
    29821 atagagatag gtttcatctt tataaaggtg gtaaacgcct ttaaacagct tgccgcagcc
    29881 aatcggccag gtgatcggtg cgcagccgat tttcagctcg ttctcaactt catcgagcag
    29941 ctccatcggg tcgcggatat cacggtcaag tttgttcata aaggtgagga tcggcgtgtc
    30001 gcgcagacgg gtaacttcca tcagcttacg ggtacgatct tcaacacctt ttgcggcgtc
    30061 gataaccatc aggcagcagt ccaccgccgt cagggtacga taggtatctt ccgagaagtc
    30121 ttcgtgcccc ggggtgtcga gcaggttaac caggcaatcg tgatacggaa actgcatcac
    30181 agacgtagta atggagatcc cacgctgctt ttccatctcc atccagtccg acttagcgtg
    30241 ctggttggaa ccacggcctt ttactgtacc ggcgctctga atggcctgtc cgaacagcag
    30301 caccttctcg gtgatggtag tcttaccggc gtccgggtga gaaataatgg caaaagtgcg
    30361 gcgcttcgcc acctcttgca aataaggaga caacgtcata attctttctt cttgagtaag
    30421 cggcatcgcg ccgcgcttgt tgaaaacgaa aaattgcggc tattttaccc atcaacgggg
    30481 gggaggcaat cagtgtttac acaggagctg ctccagttcg tgcaacgaag aaacggtcca
    30541 ggtgggcgcg atgccttctg gttgctcgcg attgtgcgca ttcagccagc aggtcgcaag
    30601 cccggcgttg atgccaccga gaatatcgga ctcagctgtg tcgccaacca tcagcacacg
    30661 tgaacggtca ggattgcctg cctgttccag cgcataatcg aaaattttct tattcggttt
    30721 ggcaacgcca acttcttcgg aaatcaccag caaatcgaaa taatcacgca ggcccgtgcg
    30781 ttccagacgc acttgctgca aggcgctaaa gccgttggtg atgatgccaa ttttagcgtt
    30841 gccacgaatg gcgttaagca gagaaaccgc gcccggcagt ggcgtacaga tttccgccat
    30901 cgcattaata aaggcttcgt tgagtttacc cggctcgacg ttcagccgtt cggcccagct
    30961 ctcaaaccgc ccgtgctgaa gctgtaatga agtgatcgcg ccgttttgat aatccaccca
    31021 cagtggcttg ttaacggcct gatagtcctg aaaatcttca gcggtaaagg tgacgctgta
    31081 atcaagaaac atccgctgca agccggtgaa tgagtcaaag gtaaacagcg tttcatcggc
    31141 atcaaagaaa atccagtccc acttcattat tccaccttgt cttacatact gattggcaac
    31201 gccatgatga tggcgtcttc gcgaccgtcc gtggtggggt agtaattgcg gcgaattgtc
    31261 gcctcgttaa agcctaaact ttcgtacagg gcaatggcgg cagcgtttga agcacggact
    31321 tccagccata gcgtcgccac gccgcgtttt tccagttcgt cgatcagatg ttccagcagc
    31381 gcccttccca atccctgacg ctgatagtca ggatcgaccg caatattgaa caatgtagct
    31441 tcatccagca ccacttgcgt aatcgcaaac gccgccattt tgccgttttg cgttaactga
    31501 aagttgagat aacgctcgcc ctggttgctg gcaaaggttt tttcactcca cggaaaggcg
    31561 tgggcgcgtt gttcaatgtg gtaagccgct ggtaaatcag tcgtttcgag ggaagaaatc
    31621 gtgttcatat gtgcaaattt gttgccataa cgcggcgcgt gccgttgggt ttgcccgtaa
    31681 ttcggtgagc gccggtgatg ccacctgagc gccttccagt gatagcggtt cgtcagtacc
    31741 caaccgccag ctgttgcagc gactgccttg cggcagcatc gcgatttttt ctggcgtcag
    31801 ttgcagcact tgatcggggc tgacggttaa cgcgcgcaga acatcgctca ctataggatc
    31861 ggtcagggcg ggaagatcgt ttgccaccat caccagacgg acgtgtgccg ggatcgcaat
    31921 ggcaatctcc ccctgcaacg cgccagggcg acgcagcgac cactgggtaa tgcccagttg
    31981 ctgtaactgc cagtctcgtc gggatgtcat agcgaatcgc tcctgttgtc aggggcgcaa
    32041 atatagcaaa ttcgtcgata ccgcgccaac atatggctat aatcgccgcc agtattaatt
    32101 gaggagcatt ccatgtctgc atttaccccg gcaagtgaag tcttgctgcg tcacagtgat
    32161 gatttcgaac aaagccgtat tctttttgcc ggagacttac aggatgacct gcccgcgcgt
    32221 ttagataccg cggccagccg tgctcatacc cagcaattcc accactggca ggtgttaagc
    32281 cgccagatgg gggataacgc ccgttttagt ctggtcgcca cggcggatga tgtcgcagat
    32341 tgcgatacgc tgatttacta ctggccgaag aacaaaccgg aagcccagtt ccagttgatg
    32401 aatttacttt ctctgctgcc agtgggcacg gatatttttg tcgttggcga gaaccgcagc
    32461 ggcgtgcgca gcgccgagca gatgctgtca gattatgcgc cgttgaataa aatggacagc
    32521 gctcgtcgct gtggcctcta ttttggtcgt ctggaaaaac agccagtatt tgatgccgat
    32581 aaattctggg gcgaatacag cgtcgatggc ctgacggtca aaacgctgcc tggcgtgttt
    32641 agccgcgacg gtctggatgt cggtagccag ttgctgctct cgacgttaac cccgcacacg
    32701 aaaggtaaag tgctggatgt cggctgtggc gcgggcgtac tttcggttgc ctttgcgcgc
    32761 cattcgccga aaattcgtct cactctgtgc gatgtctctg caccagcggt agaagccagc
    32821 cgcgcaacac ttgcggccaa cggtgttgaa ggtgaagtct ttgccagtaa cgtcttttcc
    32881 gaggtgaaag gtcgttttga tatgatcatc tccaacccgc cgttccacga tgggatgcaa
    32941 accagcctgg atgccgcgca aacgctgatt cgcggcgcgg tgcgtcatct taatagcggc
    33001 ggcgagctgc gaattgtagc gaacgccttc ctgccttatc cggacgtgct ggatgagaca
    33061 tttggcttcc acgaagtgat cgcgcaaacc gggcgcttca aggtgtatcg cgccattatg
    33121 acccgccagg cgaagaaagg ttgagtatct gatatatgtc gccgcatacc gtgtcggatg
    33181 cggcgtacac gccttatctg accgacaaac gaattatcca caatccgctg atggacattt
    33241 ttccagcaat tacacctctg tcgataatta actattgacg aaaagctgaa aaccactaga
    33301 atgcgcctcc gtggtagcaa ttctttttaa gaattgacgg tatgcgaagg tggcggaatt
    33361 ggtagacgcg ctagcttcag gtgttagtgt ccttacggac gtgggggttc aagtcccccc
    33421 cctcgcacca attatcttta cttcctttct tgtttcttcc ctgttttatc atccctgctt
    33481 caaattcatt atcatcagca tgatgccagc aataagcgcc aggcacgatg gcagcaaaac
    33541 gtaatgccgc aactgtttgt gatacagcat cgccggcgtc aatactaatc ccatcagaat
    33601 gataatccgc cattgatccg gcgataaatc cgcatacatc agccctgcaa tcagcaatcc
    33661 cggcagcaac actccccagc cactgcccag cgtacgttgc aacatggttt catctctttc
    33721 attgataatg ataaccaata tcatatgata atttttatca tttgcaagcc agagaaatcc
    33781 cttgctatcg ggtaaaccta tcgctaggat tagcaatcat tatcatttag attactatca
    33841 cgattatggc ctatcgttcc gcaccgctct atgaagatgt tatctggcga acgcatctcc
    33901 agccgcagga tgctgggctt gcacaagccg tacgggcgac gatcgccgaa catcgtgaac
    33961 atttgctgga gtttatccgt ctggatgaac ccgcaccgct ccgtgccatg acgctggcgc
    34021 aatggtcatc accgaatacg ctaagttctc aactggcggt ctattccgat catatctacc
    34081 gcaaccaacc cttgatgatc cgcgagaaca aaccgctgat ctcactatgg gcacaatggt
    34141 atatcggcct gatggtgcca ccattaatgt tggcgctgct gacgcaggaa aaagcattag
    34201 atgtgtcgcc ggaacatttc cacgttgagt ttcacgaaac cggacgcgcc gcctgtttct
    34261 gggtcgatgt gtgtgaagat aaaaacgcaa cactacattc gccgcagcag cgaatggaaa
    34321 cgttaatcag ccaggcgctt gttccggtag tgcaggcatt agaagcgacg ggagagatca
    34381 acggcaaact tatctggagc aataccggtt atttgatcaa ctggtatctg actgagatga
    34441 aacagctgct tggcgaggcg acggttgaat cgctgcgcca tgccctcttt tttgagaaaa
    34501 tgctcacgaa cggtgaagat aatccactct ggcgtaccgt ggtactgcgc gacggcctgc
    34561 tggtgcgccg cacttgctgc cagcgttatc gcttaccgga tgtgcagcaa tgtggcgatt
    34621 gtacgctgaa atgaagcact gccggggaag taaacccggc atcatgtgga ttaataggga
    34681 tgcaacacat tacttgcttc gcaatgccgc atacataaca gaatatctgc ggcctccaac
    34741 aaccctgcat cagaactgac gcccagtttc gacatcacat taaatttatg tgcacggatc
    34801 gttttgatat tgcgtttaag ctgctcggca atttgtgtca ttgagtagcc acgcgacata
    34861 aagcgcaata tttctctctc cgtcgggctt agcgttcggc tttgattaat gtaccactga
    34921 ttgttcagtc ggtcggtcgc ctgacgtaca ccatttaatg acaaaaagag ttcctgatga
    34981 aaaatctcca gcgttgacgc tttacttaat acaccgtcca acggtgatgg cgacaatgaa
    35041 ccaatcagcc gagcttcaat atcatcatcc gcaataacta aacgccgggt acgcggaaac
    35101 ttaatcgcca gttcagtcag gcaagataat ccctctctgc gctcacttct catggcagaa
    35161 aaagaaaaaa tgaccgccgc aaacgagatc cgcgacatgg catcctgaaa tgactcctga
    35221 ctggtatagg tatggagctt ataagggtta cccgcaaact ttctgaataa actctcaata
    35281 ccgatactgc tcatgacgca tttttctatg agtgcgacat ttctcttctt aattcggctg
    35341 tgttccattc tgcggcatct ccataaacag aaggcaagtt aagttctctt gcaccctgcg
    35401 cgctatttat ccatgcatac atttcagcgt tgctgtgaat tgccagtcga cgcatcgcac
    35461 tgtttttttg ggcgctgata gttttattac ttttcttaag caatgaagct atctggttga
    35521 tgccccatcc tttacctaat agccgcagta ccttgcgctc tgaaagagtg agaatgacgg
    35581 agcgatagct aaaatcatga gtttcagtca ttgccggaga ggtcagcatg gcgctgatac
    35641 gctctgcgtg cgtattgccg gaacgtacgg tcttgaccag attctcaatg ggttcaacat
    35701 cagacaataa cgtcgccgta gggcacataa gcagttctac tgcctgggaa taccaggagc
    35761 gtgacaccat gaaaacccag tgaatttccc gatattgtga gattaaagaa taataatgtt
    35821 cacaaacaga acgtggatct tcactttcac cggctaaatc agcaatgact aatccggaac
    35881 gacgtaattg taataaggta aggtcctccg cagaggcgct ggatattatt tcatattcag
    35941 ggaagtgagt cctcatgacc tcttttaacc ctgcttgcat aacaggtatt ttactgataa
    36001 caattccatt tttgcagcat cctggcaaca tatttcctcc acatccactg tctaaaaatc
    36061 atgatggttt attcaggata ttcattcctt aaatgtttcc agcaaattaa tagattaagc
    36121 gaataaatta tccttagcat tcactttcat atcagcatac tcctgctgca caacaactcc
    36181 actgcatgtg tcagaaggtt agcatcaaat gataacctgg tgaaatagcc tggagacatt
    36241 taaaatatct acattaagtt gttagaaata cttaatgaaa aacagaattc tcattcattt
    36301 aatttataat taactgatat cccttccagc agaatgcaaa atcacacaat caatatttat
    36361 tcaataaaat ctgatagttt taacttgtaa aaattaaaaa acgcgcccag ttatctttta
    36421 tcctgattcg taagggctaa acaataaatt cattatagtg ataatattca tgcgataatt
    36481 tgtttgcgac gagagataat tcgcttttta acaccgagct tctcccgata accaggatta
    36541 atcagcgcaa ttttgatctt aacctgccgc aaaacaaccg tgcttcatga tacaaatgga
    36601 ttctcattat ctgaacaata cgcagcacgt ctatgacaaa gggcgagtta tgcaaactga
    36661 gcaacagcga gccgtaacac ggctttgtat ccagtgtgga ttatttcttt tgcaacatgg
    36721 tgcggaaagc gcgttggttg atgagctttc ctcacgactg ggtcgggcgt tgggaatgga
    36781 cagcgtcgaa agttctatct cttcgaacgc catagtgctg acaactatta aagatgggca
    36841 atgcctgaca tcgacacgta aaaatcacga tcgcggcatt aatatgcatg tggtgactga
    36901 agtccagcac attgtgattc tggcggagca ccatctgctg gattacaaag gcgtagagaa
    36961 acgatttagc caaattcagc cattacgtta cccaagatgg ctggtagcct taatggttgg
    37021 cctttcttgc gcctgtttct gtaaactcaa taacggtggc tgggatggtg ccgtcatcac
    37081 cttctttgcc agtacggccg cgatgtatat ccgccagctg ctggcacaac gtcatcttca
    37141 tccacagatc aacttttgcc tcaccgcttt cgccgccacc accatttccg gtttgctttt
    37201 gcaacttccc actttcagca atacccccac catcgcgatg gccgccagcg ttctgctgct
    37261 ggtgccgggc tttccgttga ttaatgccgt cgccgatatg tttaaaggcc acatcaatac
    37321 cggactggca cgctgggcga tcgccagtct gctgacactg gctacctgcg tcggcgtggt
    37381 gatggcactg acgatttggg ggctacgcgg atgggtgtga tcgaatttct gttagcgttg
    37441 gcgcaggata tgatcctcgc cgccattcct gcggtcggct ttgcgatggt gttcaacgtt
    37501 cccgtgcggg cgttacgctg gtgtgcgttg cttggctcga taggtcatgg ttcccgaatg
    37561 atcttaatga ccagcgggct gaatattgag tggtcaactt ttatggcttc tatgctggtc
    37621 ggcaccattg gtattcaatg gtcgcgctgg tatctggcgc atccgaaagt gtttaccgtg
    37681 gcggctgtta ttcctatgtt ccctggcata tcggcttata ccgcaatgat ttcggcggta
    37741 aaaatcagcc agctaggtta cagcgaaccg ttgatgatta ccctgttaac caactttctt
    37801 acagcttcat cgattgttgg tgcgttatcc atcggtcttt ccattcctgg attatggttg
    37861 taccgcaagc gccctcgcgt ataaaattgc ctcacttaaa gcacgcggat gagagacagc
    37921 ctcctctcct ccgtgtgtta ctataaaagt tatctccctt ctcgttcatc gttccatatt
    37981 tgagaaacag tatgtcttcc agagttttga ccccggacgt cgttggtatt gacgccctgg
    38041 tacacgatca ccaaaccgtt ctggcaaaag ctgaaggcgg tgtggttgcc gtatttgcta
    38101 acaatgcccc ggcgttttat gccgttacgc ctgcacgcct ggctgaactg ctggcgctgg
    38161 aagaaaagct ggcgcgtccg ggaagcgatg tcgctctgga cgatcaactc tatcaggaac
    38221 cgcaagccgc tcccgttgct gtacccatgg ggaaattcgc catgtatccg gactggcaac
    38281 ccgatgccga ctttatccgc ctggcggcgc tatggggcgt ggcgctaaga gagccggtga
    38341 ccaccgaaga actggcctca ttcattgcct actggcaggc ggaaggtaaa gtctttcacc
    38401 atgtgcagtg gcaacaaaaa ctggcgcgca gcctgcaaat cggtcgtgcc agcaacggcg
    38461 gactgccgaa acgagatgtg aatacggtca gcgaacctga cagccaaatt ccaccaggat
    38521 tcagagggta acgatgaaaa acgttggcga cctgatgcaa cgcctgcaaa aaatgatgcc
    38581 tgcccatatc aaacctgcgt tcaaaacggg tgaagaacta ctggcctggc agaaagaaca
    38641 aggagcgatc cgctccgccg ctctcgaacg tgaaaatcgg gcgatgaaaa tgcagcgcac
    38701 ctttaaccgc tccggtattc gtccactgca tcagaactgc tcctttgaga actatcgcgt
    38761 tgagtgtgaa gggcagatga atgcgttaag caaagcgcgc cagtatgtcg aagagtttga
    38821 cggcaacatc gccagcttca tcttttctgg caagcccgga accggcaaaa accatctggc
    38881 ggcggcaatc tgcaacgagc tgctgctacg cggtaaatcc gtattgatca tcaccgtggc
    38941 cgatattatg tcggcgatga aagatacctt caggaatagc ggtaccagcg aagaacaact
    39001 gcttaacgat ctgagcaacg ttgatctgct ggtgatcgat gagatcggcg tgcagaccga
    39061 atcgaaatac gaaaaagtga tcatcaacca gatcgtcgat cgccgctctt cttccaaacg
    39121 cccaaccggg atgctgacca acagtaatat ggaagagatg accaagttgc tgggcgaacg
    39181 cgtgatggac cgtatgcgcc tgggtaacag tttgtgggtg atcttcaact gggatagcta
    39241 ccgtagccgg gtaacgggta aagagtatta atccccgttt ctggcggtat actgcgacac
    39301 aattccacct tgagtaatga tgatgaaaac tgttaaacat cttctgtgct gtgccattgc
    39361 cgccagcgca ttaatttcca ccggggtgca tgctgcgtcc tggaaagatg cgctctccag
    39421 cgccgccagc gaacttggca accaaaacag cacgacacag gaaggcggtt ggtcgctcgc
    39481 gtcattaact aacttgctta gcagcggaaa ccaggcctta agcgcagata acatgaacaa
    39541 tgccgcaggc attctgcaat actgcgcgaa gcaaaagctg gcttcggtaa ccgacgccga
    39601 aaacatcaag aatcaggtac tggaaaaact gggcctgaac agtgaagagc aaaaagaaga
    39661 caccaactat ctggatggca ttcagggttt gctgaaaacc aaagatggtc agcaactcaa
    39721 tctggataat attggaacga ctccgctggc agaaaaggtg aaaaccaaag cctgcgatct
    39781 ggtgttaaag caggggctga acttcatttc ctgaaccgcg aaacgccgcg ttttagctgt
    39841 tacccggctg acgcggctct ctcctgccct tttccctgct cctggacggt tttccccctg
    39901 ttacacggga agcgaccgcg atcaaaaaag tctgtttata aactaattcc taacaacatc
    39961 gcattttttt gacactagaa ttgcagcaat atggcgacgc cattacattg tgcttctcaa
    40021 aaagcttaaa cgttggctaa acggcccggt ttagtgagga tcgcccgttg tcagaactac
    40081 tctctttcgc cctttttctc gcctctgtgc tgatttacgc atggaaagcg ggacgtaaca
    40141 cctggtggtt tgcagccaca ttaacggtac tggggctatt tgtcgtttta aatatcaccc
    40201 tgtttgccag cgactatttt actggcgatg gtattaacga cgcggttctc tataccttaa
    40261 ccaacagcct gaccgatgct ggtgtcagta aatacatcct gcctggtatc ggcattgtgc
    40321 tggggctgac agcggtgttc ggtgcgctgg gctggatcct gcgccgtcgt cgtcatcatc
    40381 cgcaccattt tggttacagc ctgctggcac tcttactggc gttgggttca gtggatgcca
    40441 gcccggcatt tcgtcagata acggaactgg tgaaatccca gtcacgcgac ggcgacccgg
    40501 actttgcggc ttattataaa gagccgtcga aaactatccc tgacccgaaa ctcaacctgg
    40561 tttatatcta cggcgaaagt ctcgagcgga cctattttga taacgaggct ttcccggatc
    40621 tcacgcctga actgggcgcg ttgaaaaatg aaggcctgga tttcagccac acgcagcagc
    40681 tgccaggaac ggattacacg attgcgggca tggtggcttc tcagtgcgga ataccgctgt
    40741 ttgccccctt tgaaggcaac gcctccgctt ctgtttccag cttcttcccg cagaacatct
    40801 gtctgggcga tatcctgaaa aactcgggtt atcagaacta tttcgtgcag ggcgcgaatc
    40861 tgcgttttgc cggtaaagat gtgttcctga agtcgcatgg cttcgaccac ttatatggct
    40921 cagaagagct gaaaagcgtg gtggccgacc cgcactatcg caacgactgg ggattctacg
    40981 acgataccgt tctcgacgaa gcgtggaaaa agtttgaaga gctttcccgc tcaggtcagc
    41041 gattctcact gtttaccctg acagtcgata cccatcaccc ggatggtttt atctcgcgta
    41101 cctgtaaccg caaaaaatat gattttgacg gtaagccgaa tcagtcattc agcgcggtaa
    41161 gttgcagcca ggagaatatc gcgacgttta tcaacaaaat caaagcgtca ccgtggttta
    41221 aagataccgt catcgtcgtc tcttctgacc atttagcgat gaacaacacg gcgtggaaat
    41281 accttaataa acaggatcgc aataacctgt tttttgtcat ccgtggcgac aagccgcagc
    41341 aagagacgct ggcggtgaag cgtaacacga tggataacgg cgcgacggtg ctggacattc
    41401 tcggtggcga taactatctc ggacttggtc gtagcagttt atccgggcag tcaatgtcgg
    41461 aaatcttcct caatatcaaa gagaaaacat tggcgtggaa gccggatatc atccgcctgt
    41521 ggaaattccc taaagagatg aaagagttca ccatcgacca gcagaaaaac atgattgcct
    41581 tctcgggtag ccatttccgt ttgccgctac tgttgcgggt ttcagacaaa cgcgtggaac
    41641 cgctgccgga aagtgaatac tcagcaccgc tgcgtttcca gctggccgat ttcgctccac
    41701 gcgacaattt cgtctgggtt gaccgttgct acaagatggc acaactttgg gctccggaac
    41761 tggcactctc caccgactgg tgtgtctcgc aagggcagct tggcggtcag caaattgtcc
    41821 aacatgttga caaaacaaca tggaagggca aaacggcatt taaagatacg gtcatcgaca
    41881 tggcgcgtta caaaggcaat gtcgatacgc tgaagattgt tgataacgat attcgctaca
    41941 aagccgacag tttcatcttt aacgtcgccg gtgcgccaga agaggtgaaa cagtttagcg
    42001 ggatttcccg tccggagtcg tggggccgct ggtccaacgc tcagctgggc gacgaagtaa
    42061 aaatcgagta caagcatccg ctgccgaaga aatttgacct ggtgattacc gccaaagcat
    42121 acggcaataa cgccagccgt cctattccgg tacgcgtagg caatgaagaa caaactcttg
    42181 tgctgggcaa tgaagtgacc accaccacgc tgcatttcga taacccaacc gatgccgaca
    42241 cgctggtaat tgtgccgccg gaacctgtct caaccaacga agggaatatc ctcggacact
    42301 cgccgcgtaa gctagggatc ggcatggtgg aaattaaagt ggtagaacgt gaagggtaat
    42361 tcctgactga tgtgtaggtc gcataaggtg cgacctacac gccttctccg gcttacaaca
    42421 cactattaag aaacaaaaaa aacagcgccc aacacttccc cggacgctgc atggcaactt
    42481 taataatctc gccttatcag aaagtaatta cacccttaat caactcacga ttgttaatca
    42541 catcacgctc gtaggtttct gccagcgtgg cgaacgggta gcgatgggtt aacatcatgt
    42601 cggcagtgat tttcccttcc gccatcagtc gaccgacttt agcgaaatct tccggcgtgg
    42661 cgttgcggct acccatcatc gtcgtttctt ttttatggaa ttctggatcg gagaactgca
    42721 actcaccttt aaacagaccg acaaatacca ccgtgccgcc gtgacgaatc agattcacgg
    42781 tgttattcat cgcatgttga ttacctgtcg cgtcgatcac tttctgcgcc agcgaaccac
    42841 caaactgcgc ccgcagctgc gcgtcaaaat cttcggctga tgggtccagc acaggcaact
    42901 ccagacgcgt tgccacatgt tcacggcgcg ccggactggt atccgccacc gctaccagtg
    42961 cgccatcggc tttagcgatt gccgccgcgc ccagaccgat tggccccgcc ccgaccacca
    43021 gcacctgctc gccgggagca atggctgcgc gacgcaccgc atgagcgcta atagcgaaag
    43081 gttcaatcaa tgctgccgcc tgcgggtcaa taccgtctgc gggcaaaatg ttcgccaccg
    43141 gcacgctcaa atactcacta aaaccgccat cctgatgcac gccaatgact gaaatttttt
    43201 cacagcaatt ggtacgcccg cttttacacg ccgggcattg ctgacaggca acataaggga
    43261 tcacagcaac ttgctgacca tttttaagat cagcaatatt tttacccagc ccaacaatct
    43321 ccccacatat ttcatggcct aaaacgcgtg gataactaaa aaatggttga tttccacccc
    43381 aggcatgaat atcggtaccg caaatcccga cagacttaat ttttattaat gcttcattgt
    43441 cacccggaat aggtatctcg cgttgtttcc agactaattc tttcggctgc tggcaaatta
    43501 aaacattcat cgtagacatg attcggctct ccattttttg ttgccttaga tatcaatgaa
    43561 aatgaaaaat ggcagcggaa cggaaaatct gttttgtgaa aacacacgca taaaaatgtt
    43621 ttaaatcggg ttttaatgga ctcacaagga ggaaaatatg agtcgttcac aaaatttacg
    43681 ccacaatgtg attaaccagg tcattgatga tatggcgcgg ggtcatatcc cttccccact
    43741 tccatcgcaa agtgcgctgg cggaaatgta caacatcagc cgcactacag tgcgtcatat
    43801 tctcagccac ttacgcgaat gcggcgtctt aacgcaggtt ggaaacgact atgtgattgc
    43861 gcgtaaacct gaccatgatg acggtttcgc ctgtactacc gcttcaatga gtgaacaaaa
    43921 caaagtgttt gagcaggctt ttttcacgat gataaaccag cgccagttac gcccagggga
    43981 aactttttct gaactgcaac tggctcgggc cgcgggtgtc agcccggttg tagtaagaga
    44041 atatctttta aaattcggcc gttataatct tattcaaagc gaaaagcgcg gccagtggag
    44101 catgaagcaa ttcgatcaat cctacgctga gcagctgttt gagctacgcg aaatgctcga
    44161 aacacattca cttcagcact ttctcaacct gcctgatcat gatccccgct ggctgcaggc
    44221 gaaaaccatg ctcgaacgtc atcggttact gcgtgacaac atcggcaata gctttcgcat
    44281 gttctcgcaa cttgacagag attttcactc cttactgctt tcagctgccg acaatatctt
    44341 ttttgatcaa tcgcttgaga tcatctcagt catctttcat tttcactatc aatgggacga
    44401 aagcgatctt aaacagcgca atatcattgc cgtcgatgag catatgacca ttctcagtgc
    44461 tctgatttgc cgcagtgacc ttgatgccac ccttgcgctc cgtaaccatt tgaattcagc
    44521 taaacaatca atgattcgat ccattaatga gaacacgcgt tatgctcatt aagtacggat
    44581 taaaaacagc aaactattag cgggtgcacg gttttatcaa aagataaata ccgggaaatt
    44641 cccgcttacc tatgctcaca atcagtcgac taaaaatgtt tttgtgagcg gtagtaaagt
    44701 cctaaaactt taacctgccg ttaatgtcaa ccttgcgtaa tgcacaatta cgtaaacaat
    44761 taaaattata aatacaggag tccggcgtgg aaaaagaaaa tatcaccctc gatccgcgtt
    44821 cttcatttac tccatcttcg tcggcagata ttcccgtgcc accagatgga ttagttcaac
    44881 gcagtacccg aattaaacgc attcaaacca ccgccatgtt gttattattt tttgcggcgg
    44941 taatcaatta tctcgaccgc agttcgctgt cggttgcaaa tttaacgatt cgtgaagaat
    45001 tgggattaag tgctaccgaa atcggcgctt tgctctccgt gttttcactc gcttacggga
    45061 ttgcgcaact tccttgcggc ccactattgg atcgtaaagg cccacgcctg atgctgggac
    45121 tggggatgtt cttctggtca ctgttccagg ccatgtctgg catggtgcac agctttacgc
    45181 agttcgtgtt ggtgcgtatc ggtatgggga ttggtgaagc gccgatgaac ccatgcggtg
    45241 taaaagtcat taacgactgg ttcaacatca aagagcgcgg acgcccgatg ggcttcttca
    45301 acgcagcttc tactattggc gttgccgtaa gcccaccgat tctggcggcg atgatgctgg
    45361 tgatgggctg gcgtgggatg tttattacca ttggtgtgct ggggattttt ctcgccattg
    45421 gctggtatat gctctatcgc aaccgcgagc acgtagaact gactgccgtt gaacaagctt
    45481 acctcaatgc tggtagcgtc aatgcccgcc gagatccgct cagttttgcc gaatggcgca
    45541 gcctgttccg taaccgcaca atgtggggaa tgatgatcgg attcagtggc atcaactaca
    45601 ctgcgtggct gtatctggcc tggcttcctg gttacctgca aacagcctat aacctggatt
    45661 taaaaagcac agggttgatg gcggctatcc ctttcctgtt tggggctgcc gggatgctgg
    45721 tcaacggtta cgttaccgac tggctggtca aagggggaat ggctccgatt aaaagccgta
    45781 agatctgcat tattgccggg atgttctgtt ctgccgcctt tacgctggtc gtaccgcaag
    45841 cgacaacatc catgacagcg gttctgctga ttggcatggc actgttttgt attcactttg
    45901 ccggaacatc ctgctggggc ttgatccacg tcgcagttgc ttctcgcatg acggcgtcgg
    45961 tgggcagtat ccagaacttt gccagcttca tctgcgcctc tttcgcgccg atcattactg
    46021 gttttattgt tgataccacc cactcattcc gtctggcact aatcatctgc ggttgcgtca
    46081 ccgcagcggg ggcactggcg tacatcttcc tggttcgtca gccgatcaac gacccgcgta
    46141 aagattaaac tccctatgcc cgataacatt ttgcttatcg ggcattttta tggcgattaa
    46201 aacgtttccc aattctcgcc gctatctgcc acggccattt tacgcggcgt agctggcgtc
    46261 acggttttta ctacagccga tgtttcacgc tgctgttgct gctgttgctg aatccggaac
    46321 actgccactg cttcggtcag gcgactggcc tgctcttcca gcgcggcggc ggcagcggca
    46381 gattcttcca ccagcgcggc gttttgttgg gttacccggt ccatctcagc aaccgctaag
    46441 ccaacctgat cgataccacg gctctgctca tcagaagcag aagcaatttc gcccataatg
    46501 tccgtcacgc gggtcacggc actgacaatc tccgccattg tttccccggc gctttcgacc
    46561 agcgtagagc caacatccac tttccccacc gagtcttcaa tcaggctttt aatttcacga
    46621 gcagcctgag cgctacgctg ggccagatta cgcacttctc ccgcaaccac cgcaaaaccg
    46681 cgcccttgct cacccgcgcg cgctgcttca accgccgcgt tcaaagccag aatattggtc
    46741 tggaaggcaa taccgtcaat tacgctgata atatcggcga ttttctgcga actggtggag
    46801 atatcgcgca tagtctgcac cacgttatcc accactttgc cgccgcgttg cgccgtttcg
    46861 gaagcactta acgccaggtg gctggcctga cgcgcatttt cggcgttctg cttcaccgtt
    46921 gcggtcagtt gctccatgct ggctgccgtc tcttccagcg aagcggcctg ttgctcggtg
    46981 cgcgaagaga gatcgttatt gccggtggcg atttcgctgg caccgctata gatggcattg
    47041 gccccgttgc gcacatcacc gacggtacgc atcagctctc cctgcatatg gcgcaaactc
    47101 tctgccagtt gccccatctc attagagcca tccacctcaa tcggtttcac cagatcgccg
    47161 cctgcaatat gacgaatgct gtcaatcagg cgattcattg gcgcgaccag cgaggcttta
    47221 ataccgaacc agacggcgag gatgaccgcc agtacgacga tcatcacgcc taccagaatc
    47281 cacatcgcct ggctgtagga ggcattgtta tcgctgacgg cgatatcata gagccgatcg
    47341 ttttgctcca tgtaagccac atactgcttc tcgaaaccgt cctgatatcc ctgggtcggt
    47401 tgatcaaaga actcgttgat tttgcctgca cctaacagtt ggatcagctc cgccagcgca
    47461 ttgtgataaa tatcgtaatt acgtttgatc tctgacgctg cggcggtgct ctgacgcggg
    47521 tcacgcggca acgcttcgta atccgcccag tttttttccg cctgtttcag cgaaatactg
    47581 gcactctgca tcagctcagc aacggttgaa ccgctaccaa tattattctg atccatcatg
    47641 tagcggatac ccgcgcggtt gagggtgtta cgcgtctgca acaacgcaac ccagctgcca
    47701 ttcagcgtgg attgctgctg gcgaatggtt tgtaaaacag tgaaattttc tttgtcattc
    47761 tttaaggcat taaagaacag accgcctgat gtcagttgta aaaggccaaa aacggccaaa
    47821 accagcagta agctggtcac gatcttgata cgttttaaca tggtttctct ttccactaga
    47881 cagatacact ggttgtcggc ctggtggtgg aaaactttat gctgtggaaa agcgtcggca
    47941 ctgcttttgt tgcagcaaac ttgcgttttt tgtgagggtc acactctata gcggcagttt
    48001 atggtacaaa gtatcaatct tcttcgaacc agtgatactc ctatggctta tagcatcgat
    48061 atcatctcct gcattaccga acggtttatg gcgttaacag ccactgaaaa gcgtatcgca
    48121 caatttattc ttgatgacgt ctctgccgca gtagagttgc caattgctga gctggcacgg
    48181 ttaacgcaaa ccagtcaggc gtcggtgacg cgttttgccc gtgcactggg gtgcaaagat
    48241 gtccgcgagt tgaaagtcaa actggcgcag tcactggctg tcgggcagcg gtttattctc
    48301 gatgtaccgg atcttgacgg tgttcagggg atctacgaag cgattattag cgtgctggag
    48361 acgcatcgtc gttcgctgga tatgaatgcc atcagccagg ctgtaagctg gttaagtcag
    48421 gcgcggcaaa ttcttgcgtt aggaactggc ggcggttcca ctatctgttc acaggagatt
    48481 cagtaccgtt tattccgtct ggggctaccg gtggtcagtc agaatgatgc cctgatgatg
    48541 agaatgatgt gctcggcagt catgccgcag gatgtggtga ttgtgctttc gctgggcggt
    48601 tatacaccgg aaatcatcga aagtgcggca atcgcccgtc agtatggcgc gcgtgttatt
    48661 gccattacgc cagcacaaac accgctggcg gaacaggtgg atctggtatt gccgttactg
    48721 gttcaggaaa gtgattacat ctttaagccc accccttccc gctacgcgat gttagccatg
    48781 gtcgatgtac tggcaacaga actggcaatg gcaaataaag cgcaggcgaa agatcgcctg
    48841 cggcggataa aactcgtgtt agacagccac cgtgggggcg ctaaccgcca gccacttggc
    48901 gattagcatt gtcggttatg cttcttcagg tttggtgatc acgacactct ggttttcacg
    48961 ctcattggtg ataatagcca aagcttcttc ccggctgacc tgacttgctg gcgttaccaa
    49021 cgtcaccagt acgccagcga ccaggctgcc cagcactgac ggaatacacg ggttacccca
    49081 gtaagccagc cagtcggcat tcatcagcac cacaattgac atcaccatac cgctcactaa
    49141 cgccgccagc gcgccctgcc agttaaagcg tagccagaag cgaccgagaa tcgagcagat
    49201 aaacagcccg gacatcagca tcgaaatcat tttggtaatg tagctgatga tgtcattaga
    49261 ggtcagagca aagaccagcg ccaggccaat aacaaaggtc aacatccagc gtgagtaagt
    49321 aatggccttg tcctccggcg gcattctgcc ggtaatcatg gtgtagacgt cgcgcatcat
    49381 aatcgccacc gccgcgatgg catcggagct ggcagaagac atggtggctg aaagcccggc
    49441 aatcagcacc accagcccaa gaatggcagg taagaaactg gtcgcgaaca agaaggcgta
    49501 gttgctgttt tccagatttg ggttcatggt gaacgccgcc atgccgataa tggcaggcaa
    49561 aatcgagaag aacagatata acaccccagt aataacaaac gaacggcgta cagagggaac
    49621 atctttaccc gagtaaatac gctggcgata agacggtgtt gccagcacac ctacgccaat
    49681 aaccagtgcc agcgaaagcg caggcagcac gcccatttta tcaatcgcaa acaggctcat
    49741 cgcttttggg tccatcgcct gctcaatcgc actccagccg ccaacgtgaa caacggcaag
    49801 aatagccatt aatataaagc caaagaacag aattagggcc tgaatagtat ctgtccacac
    49861 taccgccgaa taaccgccga taataacata aatcgcaaaa gctaaggcga taattaattt
    49921 tgccacagta agatcaatac ccgtcgccca ggcaagatac atacttccac ctaatatatg
    49981 cgcccccagc cagccaa
//
LOCUS       DAZFLD010000009        47372 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.009, whole genome shotgun sequence.
ACCESSION   DAZFLD010000009 DAZFLD010000000
VERSION     DAZFLD010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 47372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47372
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.009"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            131..634
                     /gene="dps"
                     /locus_tag="ACHKBL_000467"
                     /gene_synonym="pexB"
     CDS             131..634
                     /gene="dps"
                     /locus_tag="ACHKBL_000467"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016722 - oxidoreductase activity, acting
                     on metal ions [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HFO5817391.1"
                     /translation="MSTAKLVKSKATNLLYTRNDVSDSEKKATVELLNRQVIQFIDLS
                     LITKQAHWNMRGANFIAVHEMLDGFRTALIDHLDTMAERAVQLGGVALGTTQVINSKT
                     PLKSYPLDIHNVQDHLKELADRYAIVANDVRKAIGEAKDDDTADILTAASRDLDKFLW
                     FIESNIE"
     gene            1038..1784
                     /gene="glnH"
                     /locus_tag="ACHKBL_000468"
     CDS             1038..1784
                     /gene="glnH"
                     /locus_tag="ACHKBL_000468"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015599 - ATPase-coupled L-glutamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1903803 - L-glutamine import across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter substrate-binding
                     protein GlnH"
                     /protein_id="HFO5817392.1"
                     /translation="MKSVLKVSLAALTLAFAVSSHAADKKLVVATDTAFVPFEFKQGD
                     KYVGFDVDLWAAIAKELKLDYELKPMDFSGIIPALQTKNVDLALAGITITDERKKAID
                     FSDGYYKSGLLVMVKANNNDVKSVKDLDGKVVAVKSGTGSVDYAKANIKTKDLRQFPN
                     IDNAYMELGTNRADAVLHDTPNILYFIKTAGNGQFKAVGDSLEAQQYGIAFPKGSDEL
                     RDKVNGALKTLRENGTYNEIYKKWFGTEPK"
     gene            1923..2582
                     /gene="glnP"
                     /locus_tag="ACHKBL_000469"
     CDS             1923..2582
                     /gene="glnP"
                     /locus_tag="ACHKBL_000469"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease GlnP"
                     /protein_id="HFO5817393.1"
                     /translation="MQFDWSAIWPAIPLLIEGAKMTLWISVLGLAGGLVIGLLAGFAR
                     TFGGWIANHVALVFIEVIRGTPIVVQVMFIYFALPMAFNDLRIDPFTAAVVTIMINSG
                     AYIAEITRGAVLSIHKGFREAGLALGLSRWETIRYVILPLALRRMLPPLGNQWIISIK
                     DTSLFIVIGVAELTRQGQEIIAGNFRALEIWSAVAVFYLIITLVLSFILRRLERRMKI
                     L"
     gene            2579..3301
                     /gene="glnQ"
                     /locus_tag="ACHKBL_000470"
     CDS             2579..3301
                     /gene="glnQ"
                     /locus_tag="ACHKBL_000470"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HFO5817394.1"
                     /translation="MIEFKNVSKHFGPTQVLHNIDLNIAQGEVVVIIGPSGSGKSTLL
                     RCINKLEEITSGDLIVDGLKVNDPKVDERLIRQEAGMVFQQFYLFPHLTALENVMFGP
                     LRVRGANKEEAEKLARELLAKVGLAERAHHYPSELSGGQQQRVAIARALAVKPKMMLF
                     DEPTSALDPELRHEVLKVMQDLAEEGMTMVIVTHEIGFAEKVASRLIFIDKGRIAEDG
                     DPQVLIKNPPSQRLQEFLQHVS"
     gene            3418..5643
                     /gene="ybiO"
                     /locus_tag="ACHKBL_000471"
     CDS             3418..5643
                     /gene="ybiO"
                     /locus_tag="ACHKBL_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel protein"
                     /protein_id="HFO5817395.1"
                     /translation="MRWILFILFCLLGAPAHAVSIPGVTTTTTTDSTTEPAPEPDIEQ
                     KKAAYGALADVLDNDTSRKELIDQLRTVAATPPAEPVPKIVPPTLVEEQTVLQKVTEV
                     SRHYGEALSARFGQLYRNITGSPHKPFNPQTFSNALTHFSMLAVLVFGFYWLIRLCAL
                     PLYRKMGQWARQKNRERSNWLQLPAMIIGAFIIDLLLLALTLFVGQVLSDNLNAGSRT
                     IAFQQSLFLNAFALIEFFKAVLRLIFCPNVAELRPFTIHDETARYWSRRLSWLSSLIG
                     YGLIVAVPIISNQVNVQIGALANVIIMLCMTIWALYLIFRNKKEITQHLLNFAEHSLA
                     FFSLFIRAFALVWHWLASAYFIVLFFFSLFDPGNSLKFMMGATVRSLAIIGIAAFVSG
                     MFSRWLAKTITLSPHTQRNYPELQKRLNGWLSAALKTARILTVCVAVMLLLSAWGLFD
                     FWNWLQNGAGQKTVDILIRIALILFFSAVGWTVLASLIENRLASDIHGRPLPSARTRT
                     LLTLFRNALAVIISTITIMIVLSEIGVNIAPLLAGAGALGLAISFGSQTLVKDIITGV
                     FIQFENGMNTGDLVTIGPLTGTVERMSIRSVGVRQDTGAYHIIPWSSITTFANFVRGI
                     GSVVANYDVDRHEDADKANQALKDAVAELMENEEIRGLVIGEPNFAGIVGLSNTAFTL
                     RVSFTTLPLKQWTVRFALDSQVKKHFDLAGVRAPVQTYQVLPAPGAAPAEPLPPGEPT
                     L"
     gene            complement(5640..6566)
                     /gene="rlmF"
                     /locus_tag="ACHKBL_000472"
     CDS             complement(5640..6566)
                     /gene="rlmF"
                     /locus_tag="ACHKBL_000472"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008988 - rRNA
                     (adenine-N6-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HFO5817396.1"
                     /translation="MSAQKPGLHPRNRHHSRYDLATLCQVNPELRQFLTLTPAGEQSV
                     DFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEASGTIP
                     ANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSAQAIISANPGLNRAIRLR
                     RQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSERKRRNLGLNKDDALNFGG
                     QQQELWCEGGEVAFIKKMIEESKGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVV
                     KKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR"
     gene            6842..7102
                     /gene="mcbA"
                     /locus_tag="ACHKBL_000473"
     CDS             6842..7102
                     /gene="mcbA"
                     /locus_tag="ACHKBL_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family periplasmic protein McbA"
                     /protein_id="HFO5817397.1"
                     /translation="MKKCLTLLIATVLSGISLTAYAAQPMSNLDSGQLRPAGTVSATG
                     ASNLSDLEDKLAEKAREQGAKGYVINSAGGNDQMFGTAIIYK"
     gene            7367..9649
                     /gene="fiu"
                     /locus_tag="ACHKBL_000474"
     CDS             7367..9649
                     /gene="fiu"
                     /locus_tag="ACHKBL_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
                     /protein_id="HFO5817398.1"
                     /translation="MENNRNFPARQFHSLTFFAGLCIGITPVAQALAAEGQANADDTL
                     VVEASTPSLYAPQQSADPKFSRPVADTTRTMTVISEQVIKDQGATNLTDALKNVPGVG
                     AFFAGENGNSTTGDAIYMRGADTSNSIYIDGIRDIGSVSRDTFNTEQVEVIKGPSGTD
                     YGRSAPTGSINMISKQPRNDSGIDASASIGSAWFRRGTLDVNQVIGDTTAVRLNVMGE
                     KTHDAGRDKVKNERYGVAPSVAFGLGTANRLYLNYLHVTQHNTPDGGIPTIGLPGYSA
                     PSAGTAALNHSGKVDTHNFYGTDSDYDDSTTDTATMRFEHDINDNTTIRNTTRWSRVK
                     QDYLMTAIMGGASNITQPTSDVNSWTWSRTANTKDVSNKILTNQTNLTSTFYTGAIGH
                     DVSTGVEFTRETQTNYGVNPVTLPAVNIYHPDSSIHPGGLTRNGANANGQTDTFAIYA
                     FDTLQITRDFELNGGIRLDNYHTEYDSATACGGSGRGAITCPAGVAKGSPVTTVDTAK
                     SGNLVNWKAGALYHLTENGNVYINYAVSQQPPGGNNFALAQSGSGNSANRTDFKPQKA
                     NTSEIGTKWQVLDKRLLLTAALFRTDIKNEVEQNDDGTYSQYGKKRVEGYEISVAGNI
                     TPAWQVIGGYTQQKATIKNGKDVAQDGSSSLPYTPEHAFTLWSQYQATDDISVGAGAR
                     YIGSMHKGSDGAVGTPAFTEGYWVADAKLGYRVNRNLDFQLNVYNLFDTDYVASINKS
                     GYRYHPGEPRTFLLTANMHF"
     gene            9691..10368
                     /gene="ybiX"
                     /locus_tag="ACHKBL_000475"
     CDS             9691..10368
                     /gene="ybiX"
                     /locus_tag="ACHKBL_000475"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase YbiX"
                     /protein_id="HFO5817399.1"
                     /translation="MMYHIPGVLSPQDVARFREQLEQAEWVDGRVTTGAQGAQVKNNQ
                     QVDTRSTLYAALQNEVLNAVNQHALFFAAALPRTLSTPLFNRYQNNETYGFHVDGAVR
                     SHPQNGWMRTDLSATLFLSDPQSYDGGELVVNDTFGQHRVKLPAGDLVLYPSSSLHCV
                     TPVTRGVRVASFMWIQSMIRDDKKRAMLFELDNNIQSLKSRYGESEEILSLLNLYHNL
                     LREWSEI"
     gene            10442..10708
                     /gene="ybiI"
                     /locus_tag="ACHKBL_000476"
     CDS             10442..10708
                     /gene="ybiI"
                     /locus_tag="ACHKBL_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc finger protein YbiI"
                     /protein_id="HFO5817400.1"
                     /translation="MASGWANDDAVNEQINSTIEDAIARARGEIPRGESLYECEECGA
                     PIPQARREAIPGVRLCIHCQQEKDLQKPAYTGYNRRGSKDSQLR"
     gene            10973..11233
                     /gene="ybiJ"
                     /locus_tag="ACHKBL_000477"
     CDS             10973..11233
                     /gene="ybiJ"
                     /locus_tag="ACHKBL_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YbiJ"
                     /protein_id="HFO5817401.1"
                     /translation="MKTINTVVAAMALSTLSFGVFAAEPVTASQAQNMNKIGVVSADG
                     ASTLDALEAKLAEKAAAAGASGYSITSATNNNKLSGTAVIYK"
     gene            complement(11373..12458)
                     /gene="hcxB"
                     /locus_tag="ACHKBL_000478"
     CDS             complement(11373..12458)
                     /gene="hcxB"
                     /locus_tag="ACHKBL_000478"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcXB"
                     /protein_id="HFO5817402.1"
                     /translation="MESGHRFDAQTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAG
                     HDSHGIGMIPSYVRSWSQGHLQINHHAKIVKEAGAVVTLDGDRAFGQVAAHEAMALGI
                     EKAHQHGIAAVALHNSHHIGRIGYWAEQCAAAGFVSIHFVSVVGIPMVAPFHGRDSRF
                     GTNPFCVVFPRKDNFPLLLDYATSAIAFGKTRVAWHKGVPVPPGCLIDVNGMPTTNPA
                     VMQESPLGSLLTFAEHKGYALAAMCEILGGALSGGKTTHQKTLQTSPDAILNCMTTII
                     INPELFGAPDCSAQTEAFAEWVKASPHDDDKPILLPGEWEVNTRRERQEQGIPLDAGS
                     WQAICDAARKVGMPEETLQAFCQQLAS"
     gene            complement(12599..13561)
                     /gene="ybiB"
                     /locus_tag="ACHKBL_000479"
     CDS             complement(12599..13561)
                     /gene="ybiB"
                     /locus_tag="ACHKBL_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HFO5817403.1"
                     /translation="MDYRKIIKEIGRGKNHARDLDRDTARGLYAHMLNGEVPDLELGG
                     VLIALRIKGEGEAEMLGFYEAMQNHTIKLTPPADKPMPIVIPSYNGARKQANLTPLLA
                     ILLHKLGFPVVVHGVSEDPTRVLTETIFELMGITPTLHGGQAQAKLDEHQPVFMPVGA
                     FCPPLEKQLAMRWRMGVRNSAHTLAKLATPFAEGEALRLSSVSHPEYIGRVAKFFSDI
                     GGRALLMHGTEGEVYANPQRCPQINLIDREGMRVLYEKQDTAGSELLPQAKDPETTAQ
                     WIKRCLAGSEPIPESLKIQMACCLMATGEAATINDGLARINQAF"
     gene            complement(13589..15739)
                     /gene="dinG"
                     /locus_tag="ACHKBL_000480"
     CDS             complement(13589..15739)
                     /gene="dinG"
                     /locus_tag="ACHKBL_000480"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HFO5817404.1"
                     /translation="MALTAALKAQIAAWYKALQEQIPDFIPRAPQRQMIADVAKTLAG
                     EEGRHLAIEAPTGVGKTLSYLIPGIAIAREEQKTLVVSTANVALQDQIYSKDLPLLKK
                     IIPDLKFTAAFGRGRYVCPRNLTALASTEPTQQDLLAFLDDELTPNNQEEQKRCAKLK
                     GDLDTYKWDGLRDHTDIAIDDDLWRRLSTDKASCLNRNCYYYRECPFFVARREIQEAE
                     VVVANHALVMAAMESEAVLPDPKNLLLVLDEGHHLPDVARDALEMSAEITAPWYRLQL
                     DLFTKLVATCMEQFRPKTIPPLAIPERLNAHCEELYELIASLNNILNLYMPAGQEAEH
                     RFAMGELPDEVLEICQRLAKLTEMLRGLAELFLNDLSEKTGSHDIVRLHRLILQMNRA
                     LGMFEAQSKLWRLASLAQSSGAPVTKWATREEREGQLHLWFHCVGIRVSDQLERLLWR
                     SIPHIIVTSATLRSLNSFSRLQEMSGLKEKAGDRFVALDSPFNHCEQGKIVIPRMRFE
                     PSIDNEEQHIAEMAAFFREQVESKKHLGMLVLFASGRAMQRFLDYVTDLRLMLLVQGD
                     QPRYRLVELHRKRVANGERSVLVGLQSFAEGLDLKGDLLSQVHIHKIAFPPIDSPVVI
                     TEGEWLKSLNRYPFEVQSLPSASFNLIQQVGRLIRSHGCWGEVVIYDKRLLTKNYGKR
                     LLDALPVFPIEQPEVPEGIVKKKEKTKSPRRRRR"
     gene            15843..16043
                     /locus_tag="ACHKBL_000481"
                     /pseudo
     CDS             15843..16043
                     /locus_tag="ACHKBL_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706676.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADAR domain-containing protein"
     gene            complement(16278..17639)
                     /gene="rhlE"
                     /locus_tag="ACHKBL_000482"
     CDS             complement(16278..17639)
                     /gene="rhlE"
                     /locus_tag="ACHKBL_000482"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlE"
                     /protein_id="HFO5817405.1"
                     /translation="MSFDSLGLSPDILRAVAEQGYREPTPIQQQAIPAVLEGRDLMAS
                     AQTGTGKTAGFTLPLLQHLITRQPHAKGRRPVRALILTPTRELAAQIGENVRDYSKYL
                     NIRSLVVFGGVSINPQMMKLRGGVDVLVATPGRLLDLEHQNAVKLDQVEILVLDEADR
                     MLDMGFIHDIRRVLTKLPAKRQNLLFSATFSDDIKALAEKLLHNPLEIEVARRNTASD
                     QVTQHVHFVDKKRKRELLSHMIGKGNWQQVLVFTRTKHGANHLAEQLNKDGIRSAAIH
                     GNKSQGARTRALADFKSGDIRVLVATDIAARGLDIEELPHVVNYELPNVPEDYVHRIG
                     RTGRAAATGEALSLVCVDEHKLLRDIEKLLKKEIPRIAIPGYEPDPSIKAEPIQNGRQ
                     QRGGGRGQGGGRGQQQPRRGEGGAKSASAKPAEKPSRRLGDAKPAGEQQRRRRPRKPA
                     AAQ"
     gene            17868..18539
                     /gene="cecR"
                     /locus_tag="ACHKBL_000483"
     CDS             17868..18539
                     /gene="cecR"
                     /locus_tag="ACHKBL_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CecR"
                     /protein_id="HFO5817406.1"
                     /translation="MNNPAMTIKGEQAKKQLIAAALAQFGEYGMNATTREIAAQAGQN
                     IAAITYYFGSKEDLYLACAQWIADFIGEQFRPHAEEAERLFAQPQPDRAAIRELILRA
                     CRNMIKLLTQDDTVNLSKFISREQLSPTAAYHLVHEQVISPLHSHLTRLIAAWTGCDA
                     SDTRMILHTHALIGEILAFRLGKETILLRTGWTAFDEEKTELINQTVTCHIDLILQGL
                     SQRSL"
     gene            18542..19537
                     /gene="hlyD"
                     /locus_tag="ACHKBL_000484"
     CDS             18542..19537
                     /gene="hlyD"
                     /locus_tag="ACHKBL_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein HlyD"
                     /protein_id="HFO5817407.1"
                     /translation="MKKPVVIGLAVVVLAAVVAGGYWWYQSRQDNGLTLYGNVDIRTV
                     NLSFRVGGRVESLAVDEGDAIKAGQVLGELDHKPYEIALMQAKAGVSVAQAQYDLMLA
                     GYRDEEIAQAAAAVKQAQAAYDYAQNFYNRQQGLWKSRTISANDLENARSSRDQAQAT
                     LKSAQDKLRQYRSGNREQDIAQAKASLEQAQAQLAQAELNLQDSTLIAPSDGTLLTRA
                     VEPGTVLNEGGTVFTVSLTRPVWVRAYVDERNLDQAQPGRKVLLYTDGRPDKPYHGQI
                     GFVSPTAEFTPKTVETPDLRTDLVYRLRIVVTDADDALRQGMPVTVQFGNEAGHE"
     gene            19530..21266
                     /gene="ybhF"
                     /locus_tag="ACHKBL_000485"
     CDS             19530..21266
                     /gene="ybhF"
                     /locus_tag="ACHKBL_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFO5817408.1"
                     /translation="MNDAVITLNGLEKRFPGMDKPAVAPLDCTIHAGYVTGLVGPDGA
                     GKTTLMRMLAGLLKPDSGSATVIGFDPIKNDGALHAVLGYMPQKFGLYEDLTVMENLN
                     LYADLRSVTGEARKQTFARLLEFTSLGPFTGRLAGKLSGGMKQKLGLACTLVGEPKVL
                     LLDEPGVGVDPISRRELWQMVHELAGEGMLILWSTSYLDEAEQCRDVLLMNEGELLYQ
                     GEPTALTQTMAGRSFLMTSPHEGNRKLLQRALKLPQVSDGMIQGKSVRLILKKETTPD
                     DIRHADGMPEININETTPRFEDAFIDLLGGAGTSESPLGAILHTVEGTPGETVIEAKE
                     LTKKFGDFAATDHVNFAVKRGEIFGLLGPNGAGKSTTFKMMCGLLVPTSGQALVLGMD
                     LKESSGKARQHLGYMAQKFSLYGNLTVEQNLRFFSGVYGLRGRAQNEKISRMSEAFGL
                     KSIASHATDELPLGFKQRLALACSLMHEPDILFLDEPTSGVDPLTRREFWLHINSMVE
                     KGVTVMVTTHFMDEAEYCDRIGLVYRGKLIASGTPDDLKAQSANDEQPDPTMEQAFIQ
                     LIHDWDKEHSNE"
     gene            21259..22392
                     /gene="ybhS"
                     /locus_tag="ACHKBL_000486"
     CDS             21259..22392
                     /gene="ybhS"
                     /locus_tag="ACHKBL_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO5817409.1"
                     /translation="MSNPILSWRRVRALCVKETRQIVRDPSSWLIAVVIPLLLLFIFG
                     YGINLDSSKLRVGILLEQRSEAALDFTHTMTGSPYIDATISDNRQELIAKMQAGKIRG
                     LVVIPVDFAEQMERANATAPIQVITDGSEPNTANFVQGYVEGIWQIWQMQRAEDNGQT
                     FEPLIDVQTRYWFNPAAISQHFIIPGAVTIIMTVIGAILTSLVVAREWERGTMEALLS
                     TEITRTELLLCKLIPYYFLGMLAMLLCMLVSVFILGVPYRGSLLILFFISSLFLLSTL
                     GMGLLISTITRNQFNAAQVALNAAFLPSIMLSGFIFQIDSMPAVIRAVTYIIPARYFV
                     STLQSLFLAGNIPVVLVVNVLFLIASAVMFIGLTWLKTKRRLD"
     gene            22403..23509
                     /gene="ybhR"
                     /locus_tag="ACHKBL_000487"
     CDS             22403..23509
                     /gene="ybhR"
                     /locus_tag="ACHKBL_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO5817410.1"
                     /translation="MFHRLWTLIRKELQSLLREPQTRAILILPVLIQVILFPFAATLE
                     VTNATIAIYDEDNGEHSVELTQRFARASAFTHVLLLKSPQEIRPTIDTQKALLLVRFP
                     ADFSRKLDTFQTAPLQLILDGRNSNSAQIAANYLQQIVKNYQQELLEGKPKPNNSELV
                     VRNWYNPNLDYKWFVVPSLIAMITTIGVMIVTSLSVAREREQGTLDQLLVSPLTTWQI
                     FIGKAVPALIVATFQATIVLAIGIWAYQIPFAGSLALFYFTMVIYGLSLVGFGLLISS
                     LCSTQQQAFIGVFVFMMPAILLSGYVSPVENMPVWLQNLTWINPIRHFTDITKQIYLK
                     DASLDIVWNSLWPLLVITATTGSAAYAMFRRKVM"
     gene            complement(23471..23881)
                     /gene="ybhQ"
                     /locus_tag="ACHKBL_000488"
     CDS             complement(23471..23881)
                     /gene="ybhQ"
                     /locus_tag="ACHKBL_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhQ family protein"
                     /protein_id="HFO5817411.1"
                     /translation="MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGL
                     CALYCVTVVMMLVFQRHPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLL
                     AIAGLAILAGPAVVSLLAKDKKLHHLTSKHRVRR"
     gene            24014..24775
                     /gene="ybhP"
                     /locus_tag="ACHKBL_000489"
     CDS             24014..24775
                     /gene="ybhP"
                     /locus_tag="ACHKBL_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFO5817412.1"
                     /translation="MPDQTQQFSFKVLTINIHKGFTAFNRRFILPELRDAVRTVSADI
                     VCLQEVMGAHEVHPLHVENWPDTSHYEFLADTMWSDFAYGRNAVYPEGHHGNAVLSRY
                     PIEHYENRDVSVDGAEKRGVLYCRIVPPMTGKAIHVMCVHLGLREAHRQAQLAMLAEW
                     VNELPDGEPVLVAGDFNDWRQKANHPLKMQAGLDEIFTRAHGRPARTFPVQFPLLRLD
                     RIYVKNASASAPTALPLRTWRHLSDHAPLSAEIHL"
     gene            24772..26013
                     /gene="clsB"
                     /locus_tag="ACHKBL_000490"
     CDS             24772..26013
                     /gene="clsB"
                     /locus_tag="ACHKBL_000490"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HFO5817413.1"
                     /translation="MKCSWREGNKIQLLENGEQYYPAMFKAIGEAQERIILETFIWFE
                     DDVGKQLHAALLAAAQRGVKAEVLLDGYGSPDLSDEFVNELTAAGVVFRYYDPRPRLF
                     GMRTNVFRRMHRKIVVIDARIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDIL
                     QFELENLPGQSAARRWWRRHHKAEENRQPGEAQVLLVWRDNEEHRDDIERHYLKMLTQ
                     ARREVIIANAYFFPGYRFLHALRKAARRGVRIKLIIQGEPDMPIVRVGARLLYNYLVK
                     GGVQVFEYRRRPLHGKVALMDDHWATVGSSNLDPLSLSLNLEANVIIHDRHFNQTLRD
                     NLNGIIAADCQQVDETMLPKRTWWNLTKSVLAFHFLRHFPALVGWLPAHTPRLAQVDP
                     PAQPTMETQDRVETENTGVKP"
     gene            26013..26969
                     /gene="ybhN"
                     /locus_tag="ACHKBL_000491"
     CDS             26013..26969
                     /gene="ybhN"
                     /locus_tag="ACHKBL_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HFO5817414.1"
                     /translation="MSKSHPRWRLAKKILTWLFFIAVIVLLVVYAKKVDWEEVWKVIR
                     DYNRVALLSAVGLVVVSYLIYGCYDLLARFYCGHKLAKRQVMLVSFICYAFNLTLSTW
                     VGGIGMRYRLYSRLGLPGSTITRIFSLSITTNWLGYILLAGIIFTAGVVELPDHWYVD
                     QTTLRILGIGLLMIIAVYLWFCAFAKHRHMTIKGQKLVLPSWKFALAQMLISSVNWMV
                     MGAIIWLLLGQSVNYFFVLGVLLVSSIAGVIVHIPAGIGVLEAVFIALLAGEHTSKGS
                     IIAALLAYRVLYYFIPLLLALICYLLLESQAKKLRAKNEAAM"
     gene            complement(27005..27394)
                     /locus_tag="ACHKBL_000492"
     CDS             complement(27005..27394)
                     /locus_tag="ACHKBL_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5817415.1"
                     /translation="MESYSQSSNKLDFQHEARILNGIWLITALGLVTTAGLALVIIRN
                     AALMSERPIWVISCLMIVLWSGIISHGRNKLLELAGKCHSEELWSPVSCAFTGALTLY
                     YYFIGFFGILAAIAITLVWQRHTRFFH"
     gene            complement(27600..28304)
                     /gene="ybhL"
                     /locus_tag="ACHKBL_000493"
     CDS             complement(27600..28304)
                     /gene="ybhL"
                     /locus_tag="ACHKBL_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFO5817416.1"
                     /translation="MDRFPRSDSIVQPRAGLQTYMAQVYGWMTVGLLLTAFVAWYAAN
                     SAAVMELLFTNRVFLIGLIIAQLALVIVLSAMIQKLSAGVTTMLFMLYSALTGLTLSS
                     IFIVYTAASIASTFVVTAGMFGAMSLYGYTTKRDLSGFGNMLFMALIGIVLASLVNFW
                     LKSEALMWAVTYIGVIVFVGLTAYDTQKLKNMGEQIDTRDTSNLRKYSILGALTLYLD
                     FINLFLMLLRIFGNRR"
     gene            complement(28441..28893)
                     /gene="moaE"
                     /locus_tag="ACHKBL_000494"
     CDS             complement(28441..28893)
                     /gene="moaE"
                     /locus_tag="ACHKBL_000494"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HFO5817417.1"
                     /translation="MAETKIVVGPQPFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLG
                     DSVKALTLEHYPGMTEKALAEIVDEARNRWPLGRVTVIHRIGELWPGDEIVFVGVTSA
                     HRSSAFEAGQFIMDYLKTRAPFWKREATPEGDRWVEARESDQQAAKRW"
     gene            complement(28895..29140)
                     /gene="moaD"
                     /locus_tag="ACHKBL_000495"
     CDS             complement(28895..29140)
                     /gene="moaD"
                     /locus_tag="ACHKBL_000495"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HFO5817418.1"
                     /translation="MIKVLFFAQVRELVGTDATEVAADFPTVEALRQHLAAQSDRWAL
                     ALEDGKLLAAVNQTLVSLDHPLTDGDEVAFFPPVTGG"
     gene            complement(29133..29618)
                     /gene="moaC"
                     /locus_tag="ACHKBL_000496"
     CDS             complement(29133..29618)
                     /gene="moaC"
                     /locus_tag="ACHKBL_000496"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061799 - cyclic pyranopterin
                     monophosphate synthase activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HFO5817419.1"
                     /translation="MSQLTHINAAGEAHMVDVSAKAETVREARAEAFVTMRSETLAMI
                     IDGRHHKGDVFATARIAGIQAAKRTWDLIPLCHPLMLSKVEVNLQAEPEHNRVRIETL
                     CRLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMVIGPVRLLAKSGGKSGDFKVEAD
                     D"
     gene            complement(29621..30133)
                     /gene="moaB"
                     /locus_tag="ACHKBL_000497"
     CDS             complement(29621..30133)
                     /gene="moaB"
                     /locus_tag="ACHKBL_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HFO5817420.1"
                     /translation="MSQVSTEFIPTRIAILTVSNRRGEEDDTSGHYLRDSAQEAGHHV
                     VDKAIVKENRYAIRAQVSAWIASDDVQVVLITGGTGLTEGDQAPEALLPLFDREVEGF
                     GEVFRMLSFEEIGTSTLQSRAVAGVANKTLIFAMPGSTKACRTAWENIIAPQLDARTR
                     PCNFHPHLKK"
     gene            complement(30155..31144)
                     /gene="moaA"
                     /locus_tag="ACHKBL_000498"
     CDS             complement(30155..31144)
                     /gene="moaA"
                     /locus_tag="ACHKBL_000498"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HFO5817421.1"
                     /translation="MASQLTDAFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNK
                     GFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDFTDIIAAVRENDAIRQIAVTTNG
                     YRLERDVANWRDAGLTGINVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEAGFEKVK
                     VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELL
                     RRGWIHQLRQRSDGPAQVFCHPDYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLF
                     GEGGVNLRDLLEDDTQQQALEARISAALREKKQTHFLHQNNTGITQNLSYIGG"
     regulatory      complement(31132..31276)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            31541..32449
                     /gene="yvcK"
                     /locus_tag="ACHKBL_000499"
     CDS             31541..32449
                     /gene="yvcK"
                     /locus_tag="ACHKBL_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HFO5817422.1"
                     /translation="MRNRTLADLDRVVALGGGHGLGRVLSSLSSLGSRLTGIVTTTDN
                     GGSTGRIRRSEGGIAWGDMRNCLNQLITEPSVASAMFEYRFGGNGELSGHNLGNLMLK
                     ALDHLSVRPLEAINLIRNLLKVDAHLIPMSEHPVDLMAIDDQGHEVYGEVNIDQLTTP
                     IQELLLTPNVPATREAVHAISEADLIIIGPGSFYTSLMPILLLKEIAQALRRTPAPMV
                     YIGNLGRELSLPAANLKLESKLAIMEQYVGKKVIDAVIVGPKVDVSAVKERIVIQEVL
                     EASDIPYRHDRQLLHSALEKALQALG"
     gene            complement(32641..34662)
                     /gene="uvrB"
                     /locus_tag="ACHKBL_000500"
     CDS             complement(32641..34662)
                     /gene="uvrB"
                     /locus_tag="ACHKBL_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HFO5817423.1"
                     /translation="MSKPFKLNSAFKPSGDQPEAIRRLEEGLEDGLAHQTLLGVTGSG
                     KTFTIANVIADLQRPTMVLAPNKTLAAQLYGEMKEFFPENAVEYFVSYYDYYQPEAYV
                     PSSDTFIEKDASVNEHIEQMRLSATKAMLERRDVVVVASVSAIYGLGDPDLYLKMMLH
                     LTVGMIIDQRAILRRLAELQYARNDQAFQRGTFRVRGEVIDIFPAESDDIALRVELFD
                     EEVERLSLFDPLTGQIVSTIPRFTIYPKTHYVTPRERIVQAMEEIKEELAARRKVLLE
                     NNKLLEEQRLTQRTQFDLEMMNELGYCSGIENYSRFLSGRGPGEPPPTLFDYLPADGL
                     LVVDESHVTIPQIGGMYRGDRARKETLVEYGFRLPSALDNRPLKFEEFEALAPQTIYV
                     SATPGNYELEKSGGDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIRQRAAINERVL
                     VTTLTKRMAEDLTEYLEEHGERVRYLHSDIDTVERMEIIRDLRLGEFDVLVGINLLRE
                     GLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNVNGKAILYGDKITPSMAKAIG
                     ETERRREKQQKYNEEHGITPQGLNKKVVDILALGQNIAKTKAKGRGKSRPIVEPDNVP
                     MDMSPKALQQKIHELEGLMMQHAQNLEFEEAAQIRDQLHQLRELFIAAS"
     gene            complement(35241..35918)
                     /gene="bioD"
                     /locus_tag="ACHKBL_000501"
     CDS             complement(35241..35918)
                     /gene="bioD"
                     /locus_tag="ACHKBL_000501"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HFO5817424.1"
                     /translation="MSKCYFVTGTDTEVGKTVASCALLQAAKAAGYRTAGYKPVASGS
                     EKTPKGLRNSDALALQRNSSLQLDYATVNPYTFAEPTSPHIISAQEGRPIESLVMSAG
                     LRALEQQADWVLVEGAGGWFTPLSDTFTFADWVTQEQLPVILVVGVKLGCINHAMLTA
                     QAILHAGLTLAGWVANDVTPPGKRHAEYMTTLTRMIPAPLLGEIPWLAENPENAATGK
                     YINLALM"
     gene            complement(35911..36666)
                     /gene="bioC"
                     /locus_tag="ACHKBL_000502"
     CDS             complement(35911..36666)
                     /gene="bioC"
                     /locus_tag="ACHKBL_000502"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010340 - carboxyl-O-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HFO5817425.1"
                     /translation="MATVNKQAIAAAFGRAAAHYEQHADLQRQSADALLAMLPQRKYT
                     HVLDAGCGPGWMSRRWRERHAQVTALDLSPPMLVQARQKDAADHYLAGDIESLPLATA
                     TFDLAWSNLAVQWCGNLSMALRELYRVVRPGGVVAFTTLVQGSLPELHQAWQAVDERP
                     HANRFLPPDKIEQSLNAVHYQHYIQPITLWFDDALSAMRSLKGIGATHLHEGRDPRIL
                     TRSQLQRLQLAWPQQQGRYPLTYHLFLGVIARE"
     gene            complement(36653..37807)
                     /gene="bioF"
                     /locus_tag="ACHKBL_000503"
     CDS             complement(36653..37807)
                     /gene="bioF"
                     /locus_tag="ACHKBL_000503"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HFO5817426.1"
                     /translation="MSWQEKINAALDARRAADALRRRYPVAQGAGRWLVADDRQYLNF
                     SSNDYLGLSHHPQIIRAWKQSAEQFGVGSGGSGHVSGYSVAHQALEEELAEWLGYSRA
                     LLFISGFAANQAVIAAMMAKEDRIVADRLSHASLLEAASLSPSPLRRFAHNDVTHLAR
                     LLASPCPGQQLVVTEGVFSMDGDSAPLEEIQQVTQQHDGWLMVDDAHGTGVIGEQGRG
                     SCWLQKVKPELLVVTFGKGFGVSGAAVLCSDTVADYLVQFARHLIYSTSMPPAQAQAL
                     RASLAVIRSDEGDTRREKLAALITRFRAGVQDLPFTLADSCSAIQPLIVGDNSRALQL
                     AEKLRQQGCWVTAIRPPTVPAGTARLRLTLTAAHEMQDIDRLLEVLHGNG"
     gene            complement(37804..38844)
                     /gene="bioB"
                     /locus_tag="ACHKBL_000504"
     CDS             complement(37804..38844)
                     /gene="bioB"
                     /locus_tag="ACHKBL_000504"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HFO5817427.1"
                     /translation="MAHRPRWTLSQVTELFEKPLLDLLFEAQQVHRQHFDPRQVQVST
                     LLSIKTGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLASARKAKAAGSTRFCMGAAW
                     KNPHERDMPYLEQMVQGVKAMGLEACMTLGTLSESQAQRLANAGLDYYNHNLDTSPEF
                     YGNIITTRTYQERLDTLEKVRDAGIKVCSGGIVGLGETVKDRAGLLLQLANLPTPPES
                     VPINMLVKVKGTPLADNDDVDAFDFIRTIAVARIMMPTSYVRLSAGREQMNEQTQAMC
                     FMAGANSIFYGCKLLTTPNPEEDKDLQLFRKLGLNPQQTAVLAGDNEQQQRLEQALMT
                     PDTDEYYNAAAL"
     gene            38931..40220
                     /gene="bioA"
                     /locus_tag="ACHKBL_000505"
     CDS             38931..40220
                     /gene="bioA"
                     /locus_tag="ACHKBL_000505"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HFO5817428.1"
                     /translation="MTTDDLAFDQRHIWHPYTSMTSPLPVYPVASAEGCELILSDGRR
                     LVDGMSSWWAAIHGYNHPQLNAAMKTQIDAMSHVMFGGITHAPAIELCRKLVAMTPQP
                     LECVFLADSGSVAVEVAMKMALQYWQAKGEARQRFLTFRNGYHGDTFGAMSVCDPDNS
                     MHSLWKGYLPENLFAPAPQSRMDGEWDERDMVGFARLMAAHRHEIAAVIIEPIVQGAG
                     GMRIYHPEWLKRIRKMCDREGILLIADEIATGFGRTGKLFACEHADIAPDILCLGKAL
                     TGGTMTLSATLTTREVAETISNGEAGCFMHGPTFMGNPLACAAANASLAILESGDWQH
                     QVAAIEGQLREQLAPACDAEMVADVRVLGAIGVVETTRPVNMAALQKFFVEQGVWIRP
                     FGKLIYLMPPYIILPQQLQRLTVAVNRAVQDETFFCQ"
     gene            40279..40755
                     /gene="ybhB"
                     /locus_tag="ACHKBL_000506"
     CDS             40279..40755
                     /gene="ybhB"
                     /locus_tag="ACHKBL_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase inhibitor"
                     /protein_id="HFO5817429.1"
                     /translation="MKLISNDLRDGDKLPHRHVFNGMGYDGDNISPHLAWDDVPAGTK
                     SFVVTCYDPDAPTGSGWWHWVVVNLPADTRVLPQGFGSGLVAMPDGVLQTRTDFGKTG
                     YDGAAPPKGETHRYIFTVHALDVERIDVDEGASGAMVGFNIHFHSLASASITAMFS"
     gene            40907..42190
                     /gene="ybhC"
                     /locus_tag="ACHKBL_000507"
     CDS             40907..42190
                     /gene="ybhC"
                     /locus_tag="ACHKBL_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative acyl-CoA thioester hydrolase"
                     /protein_id="HFO5817430.1"
                     /translation="MNTFSVSRLALALAFGVTLTACSSTPPDQRPSDQTAPGTSSRPI
                     LSAKEAQNFDAQHYFASLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQAAVD
                     AAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPA
                     DWRHDVNPRGKYMPGKPAWYMYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENT
                     LGDSVDAGNHPAVALRTDGDQVQINNVNILGRQNTFFVTNSGVQNRLETNRQPRTLVT
                     NSYIEGDVDIVSGRGAVVFDNTEFRVVNSRTQQEAYVFAPATLSNIYYGFLAVNSRFN
                     ASGDGVAQLGRSLDVDANTNGQVVIRDSAINEGFNTAKPWADAVISNRPFAGNTGSVD
                     DNDEVQRNLNDTNYNRMWEYNNRGVGSKVVAEAKK"
     gene            complement(42331..44592)
                     /gene="ybhJ"
                     /locus_tag="ACHKBL_000508"
     CDS             complement(42331..44592)
                     /gene="ybhJ"
                     /locus_tag="ACHKBL_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HFO5817431.1"
                     /translation="MIKLSEKGVFLASNNEIIAEEHFTGEIKKEEAKKGTIAWSILSS
                     HNTSGNMDKLKIKFDSLASHDITFVGIVQTAKASGMERFPLPYVLTNCHNSLCAVGGT
                     INGDDHVFGLSAAQRYGGIFVPPHIAVIHQYMREMMAGGGKMILGSDSHTRYGALGTM
                     AVGEGGGELVKQLLNDTWDIDYPGVVAVHLTGKPAPYVGPQDVALAIIGAVFKNGYVK
                     NKVMEFVGPGVAALSTDFRNSVDVMTTETTCLSSVWQTDEEVHNWLALHGRGQDYCQL
                     NPQPMAYYDGCISVDLSAIKPMIALPFHPSNVYEIDTLNQNLTDILREIEIESERVAH
                     GKAKLSLLDKVENGRLKVQQGIIAGCSGGNYENVIAAANALRGQSCGNDTFSLAVYPS
                     SQPVFMDLAKKGVVADLIGAGAIIRTAFCGPCFGAGDTPINNGLSIRHTTRNFPNREG
                     SKPANGQMSAVALMDARSIAATAANGGYLTSASELDCWDNVPEYAFDVTPYKNRVYQG
                     FVKGATQQPLIYGPNIKDWPELGALTDNIVLKVCSKILDEVTTTDELIPSGETSSYRS
                     NPIGLAEFTLSRRDPGYVGRSKATAELENQRLAGNVSELTEVFARIKQIAGQEHIDPL
                     QTEIGSMVYAVKPGDGSAREQAASCQRVIGGLANIAEEYATKRYRSNVINWGMLPLQM
                     AEAPTFEVGDYIYIPGIKAALDNPGTTFKGYVIHEDAPVTEITLYMESLTAEEREIIK
                     AGSLINFNKNRQM"
     gene            complement(44775..46208)
                     /gene="ybhI"
                     /locus_tag="ACHKBL_000509"
     CDS             complement(44775..46208)
                     /gene="ybhI"
                     /locus_tag="ACHKBL_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HFO5817432.1"
                     /translation="MNKKSLWKLILILAIPCIIGFMPAPAGLSELAWVLFGIYLAAIV
                     GLVIKPFPEPVVLLIAVAASMVVVGNLSGGEFKTTAVLSGYSSGTTWLVFSAFTLSAA
                     FVTTGLGKRIAYLLIGKIGSTTLGLGYVTVFLDLVLAPATPSNTARAGGIVLPIINSV
                     AVALGSEPEKSPRRVGHYLMMSIYMVTKTTSYMFFTAMAGNILALKMINDILHLQISW
                     GGWALAAGLPGIIMLLVTPLVIYTMYPPEIKKVDNKTIAKAGLAELGPMKIREKMLLG
                     VFVLALLGWIFSKSLGVDESTVAIVVMATMLLLGIVTWEDVVKNKGGWNTLIWYGGII
                     GLSSLLSKVKFFEWLAEVFKNNLAFDGHGNVAFFVIIFLSIIVRYFFASGSAYIVAML
                     PVFAMLANVSGAPLMLTALALLFSNSYGGMVTHYGGAAGPVIFGVGYNDIKSWWLVGA
                     VLTILTFLVHITLGVWWWNMLIGWNML"
     gene            complement(46284..47336)
                     /gene="ybhH"
                     /locus_tag="ACHKBL_000510"
     CDS             complement(46284..47336)
                     /gene="ybhH"
                     /locus_tag="ACHKBL_000510"
                     /EC_number="5.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalomesaconate tautomerase"
                     /protein_id="HFO5817433.1"
                     /translation="MKKIPCVMMRGGTSRGAFLLAEHLPEDQTQRDKILMAIMGSGND
                     LEIDGIGGGNPLTSKVAIISRSSDPRADVDYLFAQVIVHEQRVDTTPNCGNMLSGVGA
                     FAIENGLIAATSPVTRVRIRNVNTGTFIEADVQTPNGVVEYEGSARIDGVPGTAAPVA
                     LTFLNAAGTKTGKVFPTDNQIDYFDDVPVTCIDMAMPVVIIPAEYLGKTGYELPAELD
                     ADKALLASIESIRLQAGKAMGLGDVSNMVIPKPVLISPAQKGGAINVRYFMPHSCHRA
                     LAITGAIAISCSCALEGTVTRQIVPSVGYGNINIEHPSGALDVHLSNEGQDATTLRAS
                     VIRTTRKIFSGEVYLP"
BASE COUNT        11228 a        12661 c        12152 g        11331 t
ORIGIN      
        1 ttggttttgt tttttcacgc ttgttaccac tattagtgtg ataggaacag ccagaatagc
       61 ggaacacata gctggtgcta tacttagtct cgttaattac tgggacataa catcaagagg
      121 atatgaaatt atgagtaccg ctaaattagt taaatcaaaa gcgaccaatc tgctttatac
      181 ccgcaacgat gtctctgata gcgagaaaaa agcaacagta gagttgctga atcgccaggt
      241 tatccagttt attgatcttt ctttgattac caaacaagcg cactggaaca tgcgcggcgc
      301 taacttcatt gccgtacatg aaatgctgga tggcttccgc actgcactga tcgatcatct
      361 ggataccatg gcagaacgtg cagtgcagct gggcggtgta gctctgggga ccactcaagt
      421 tattaacagc aaaactccgc tgaaaagtta cccgctggac atccacaacg ttcaggatca
      481 cctgaaagag ctagctgacc gttacgcaat cgtcgctaat gacgtacgca aagcgattgg
      541 cgaagcgaaa gatgacgaca ccgcagatat cctgaccgcc gcgtctcgcg acctggataa
      601 attcctgtgg tttatcgaat ctaacatcga ataaatccat cgctgatggt gcagaacttt
      661 agtacacgat aaaggcggct tcctgacagg aggccgtttt gttttgtagc ccgcctcaac
      721 gcacttattt agtgcatcca tctgctatct tcagctgatt aagtaaattt tttgtatcca
      781 catcatcaca caatcgttac ataaagattg ttttttcatc aggttttacg ctaaataatc
      841 actgtgttga gtgcacaatt ttagcgcacc agtttggtgc cccagaatgg tgcatcttca
      901 gggtattgcc ctataaatcg tgcatcacgt ttttgccgca tctcgaaaaa tcaatgagtt
      961 gcaaaactgg cacgattttt tcatatatgt gaatgtcacg caggggatcg tcccgtggat
     1021 agaaaaaagg aaatgctatg aagtctgtat taaaagtttc actggctgca ctgaccctgg
     1081 cttttgcggt ttcttctcat gccgcggata aaaaattagt tgtcgcgacg gataccgcct
     1141 tcgttccgtt tgaatttaaa cagggcgata aatacgtggg ctttgacgtt gatctgtggg
     1201 ctgccatcgc taaagaactg aagctggatt acgaactgaa gccgatggat ttcagtggga
     1261 tcattccggc actgcaaacc aaaaacgtcg atctggcgct ggcgggcatt accatcaccg
     1321 acgagcgtaa aaaagcgatc gatttctctg acggctacta caaaagcggc ctgttagtga
     1381 tggtgaaagc caacaataac gatgtgaaaa gcgtgaaaga tctcgacggg aaagtggttg
     1441 ctgtgaagag cggcactggc tccgttgatt acgcgaaagc aaacatcaaa actaaagatc
     1501 tgcgtcagtt cccgaacatc gataacgcct atatggaact gggcaccaac cgcgcagacg
     1561 ccgttctgca cgatacgcca aacattctgt acttcatcaa aaccgccggt aacggtcagt
     1621 tcaaagcggt aggtgactct ctggaagcac agcaatacgg tattgcgttc ccgaaaggta
     1681 gcgacgagct gcgtgataaa gtcaacggcg cgttgaaaac cctgcgcgag aacggaactt
     1741 acaacgaaat ctacaaaaaa tggttcggta ctgaaccgaa ataataacgc tacgcctgta
     1801 aggcgcactg gcagttccct ctcccctctg gggagaggat tagggtgagg ggcgcaaacc
     1861 cgctccgggg ccattaatta ccctgaattt gattatttac accacggtaa caggaacaac
     1921 atatgcagtt tgactggagt gccatctggc ctgccattcc gcttctgatt gaaggtgcca
     1981 aaatgaccct gtggatctcg gtcctcggtc tggctggcgg cctggtgatc ggattgctgg
     2041 caggttttgc acgcaccttc ggtggttgga tagccaacca cgtcgcgctg gtctttattg
     2101 aagtgatccg cggcacacct atcgtcgtcc aggtgatgtt tatctatttc gccctgccga
     2161 tggcatttaa cgacttacgc atcgacccat ttactgcggc ggtggtcacc atcatgatca
     2221 actccggcgc atacattgcg gaaatcacgc gtggtgcggt gctgtctatt cacaaaggtt
     2281 ttcgtgaagc aggactggcg ctcggtcttt cacgttggga aaccattcgc tacgtcattt
     2341 taccgctggc actgcgtcgt atgctgccgc cgctgggtaa ccagtggatc atcagcatta
     2401 aagacacctc gctgtttatt gttatcggcg tggcggaact gacccgtcag gggcaagaaa
     2461 ttattgccgg taacttccgc gcccttgaga tctggagcgc cgtggcggtg ttctatctga
     2521 ttattaccct ggtgctgagc tttattctgc gtcgtctgga aagaaggatg aaaatcctgt
     2581 gattgaattt aaaaacgtct ccaagcattt tggtccaacc caggtgctgc acaatatcga
     2641 tttgaacatt gctcagggcg aagtcgtggt gattatcggg ccgtccggtt ccggtaaatc
     2701 gaccctgctg cgctgcatca acaaactgga agaaatcact tccggcgatc tgattgtcga
     2761 tggcctgaag gttaacgatc cgaaagttga cgagcgcctg attcgccagg aagcaggtat
     2821 ggtgttccag cagttttacc tcttcccgca tctgacggcg ctggaaaacg tcatgtttgg
     2881 tccgctacgc gtgcgtggcg cgaacaaaga agaggcggaa aaactggcgc gtgagctgct
     2941 ggcgaaagtc ggtctggcag aacgtgcaca tcactaccct tccgaacttt ctggtggtca
     3001 acagcagcgt gtggcgatcg cacgcgcgct ggcggtgaag ccgaaaatga tgctgtttga
     3061 tgaaccgact tccgctcttg acccggaact gcgccatgaa gtgctgaagg ttatgcagga
     3121 tctggctgaa gaagggatga cgatggtgat cgtgacacac gaaatcggtt ttgccgagaa
     3181 agtagcttcg cgcctgatct ttatcgacaa aggccggatt gcggaagatg gcgatccgca
     3241 ggtgttgatc aagaacccac cgagccagcg cttgcaggaa tttttgcagc acgtctctta
     3301 acttctcagc ctccctttcc tccgaaaggg aggcacacca gattcctctc atttaaaatc
     3361 gcccctcctc cagcatctat acttatcttt ttgctctgtt ttctcactgg aggagtcatg
     3421 cggtggatcc tgttcatcct cttctgcctg ctgggcgcac ctgcccacgc ggtatccata
     3481 cccggcgtta caaccacaac gacaacggac tcaacgactg aaccggcccc ggaaccggat
     3541 atcgaacaaa aaaaagcggc ctatggtgca ctggcggatg tgctggataa tgacacctca
     3601 cgtaaagagt tgatcgacca gttgcgcacc gtcgccgcga cgccccctgc ggaacccgta
     3661 ccgaagatcg tgccgccgac gctggttgaa gagcaaaccg tgctgcaaaa agtcaccgaa
     3721 gtcagccgcc attatggtga agccctttcc gcccgcttcg gacaacttta tcgcaatatc
     3781 accggctccc cgcataagcc gtttaatcca caaaccttca gcaatgcgtt gacccatttt
     3841 tcaatgttag cggtattagt gtttggtttt tactggctga ttcgcctgtg cgcacttccg
     3901 ctgtatcgca aaatgggcca gtgggcgcgg caaaaaaatc gtgagcgcag taactggttg
     3961 cagcttccgg cgatgattat cggggcgttt attatcgacc tgctgttact ggcactgaca
     4021 ttatttgtcg gtcaggtttt aagcgacaac ctgaatgcgg gcagtcgcac catcgctttc
     4081 caacaaagtt tgtttctcaa cgcctttgcc ctgattgaat ttttcaaagc tgtactacgc
     4141 ctgatttttt gcccaaatgt ggcggagctg cgcccgttca cgattcatga cgagaccgcc
     4201 cgttactgga gccgtcgcct gagctggtta agcagtttga taggttatgg cctgattgtg
     4261 gccgtgccga ttatctctaa tcaggtgaat gtacaaattg gcgcgctggc gaacgtcatc
     4321 attatgctgt gcatgaccat ctgggcgttg tacctgatct ttcgtaataa aaaagagatt
     4381 acccaacatt tgctcaactt tgcggagcat tcgctggcct ttttcagctt gtttatccgc
     4441 gcctttgcgc tggtgtggca ctggctggca agcgcctatt ttatcgtgct gtttttcttt
     4501 tcgttgttcg atccgggcaa cagcctgaaa tttatgatgg gtgcaacggt gcgcagcctg
     4561 gcgattattg gtatcgcagc atttgtttcc ggtatgtttt cccgctggct ggcgaaaacc
     4621 atcaccctct cgccacatac tcagcgaaat tatccggagc tgcaaaaacg gctgaatggc
     4681 tggctttcgg cggcgctgaa aacggcgcgt attctgaccg tctgcgtggc ggtaatgctg
     4741 ctgttgagcg catggggatt attcgatttc tggaactggc tgcaaaacgg tgcggggcag
     4801 aaaaccgtag atatcctgat ccgtatcgca ctcattcttt tcttctcggc ggttggctgg
     4861 acggtgctcg ccagtttgat cgaaaaccgg ctggcttcgg atattcatgg ccgcccacta
     4921 cccagcgccc gtacgcgtac cctgctgacg ctgttccgta acgcgctggc ggtgattatc
     4981 agtaccatca ccatcatgat tgtgttgtcg gaaatcggcg ttaatatcgc gccattgctg
     5041 gcaggtgccg gggcattagg tctggctatc tcttttggtt cgcaaacgct ggtgaaagat
     5101 attatcaccg gggtatttat tcagtttgaa aacggcatga acactggaga tttggtgact
     5161 atcgggccgt tgaccggcac tgtggaacgg atgtcgattc gctccgtggg cgtgcgccag
     5221 gataccgggg cgtatcacat cattccgtgg tcttcgataa ccacctttgc taacttcgtc
     5281 cgcggtattg gttcggtagt ggcaaactac gatgttgatc gccatgaaga tgctgataaa
     5341 gccaatcagg cactgaaaga tgcggtagca gaattgatgg aaaacgaaga aattcgcggg
     5401 ctggttattg gtgaaccgaa ctttgccggg attgtcggct taagcaatac cgcgtttaca
     5461 ctgcgtgttt cgttcaccac gctgccactc aaacagtgga cggtccgctt tgccctcgac
     5521 agccaggtga aaaaacattt cgacctggcg ggtgttcgcg cgccggtgca gacttatcag
     5581 gtgctgccag ctccgggcgc ggccccggct gaaccgttgc cgccggggga gccaacgctt
     5641 taacgctggc gattgacaaa acggcggcgc tgctcgtcgt tcataaaggt ccaggctata
     5701 aagcggctct gcttttgccc ctgggccatc tcttttttta ccaccttcac cgcacctacg
     5761 tccgtcaaag cacgatacaa cggcggtaag ttttcaccac gagataccag cgaggtaaac
     5821 cacatcacct gcttcgcgaa gcctttgctc tcttcaatca tctttttgat aaaagcgacc
     5881 tcaccgcctt cacaccacaa ctcctgttgc tgaccaccaa agttcagtgc atcgtctttg
     5941 ttcagcccca ggttacggcg tttacgttca ctacctgccc gtgccgcagc ggcggaatcg
     6001 tggaatggcg ggttacacaa ggtcgcatcg tattgctcgt ttttatggat gatgccgtta
     6061 aaaatcgccc cactctcttt ttgccgacgc agacgaatgg cgcggttaag ccccggatta
     6121 gcactgatga tcgcctgcgc actgcttaac gcctggctgc tggtttcact accggtaaaa
     6181 cgccagccat attcatgtac gccaatcagc ggataaatac agttcgcacc aacaccgata
     6241 tccagaatgc tggcattagc tggaattgtt ccgcttgctt cagccagtaa atcggcaagg
     6301 tgatgaatat aatccgcccg gcccggtact ggcgggcaga gaaaaccgtc cgggatatcc
     6361 cagttcgcta cggcgtaaaa atgggccagc aacgccttat tgagcgcctt caccgccagc
     6421 ggattggcaa agtctacgct ttgctccccg gcgggtgtaa gcgtgaggaa ttgcctgagt
     6481 tcaggattga cctgacaaag cgtggcgaga tcgtagcggc tgtgatgacg gttgcgcgga
     6541 tgcaaccccg gtttctgggc ggacatagca ttctccttta agcagcggcg taagataccc
     6601 gcataacgcg acagggtaaa tatctggctg cgtttatcat ttctaaaaac cttcacatgg
     6661 ctatggttta tggtaattca ccataaaaat catcatcatg catgtcattg atttatctta
     6721 ttttgtaaaa atatattgtt caaaaaataa gcgcatctct gtcaattttt tgcgtcattt
     6781 tcatcgcgca ggcgacctac acttatctgg cactacccgt tgtatttatg aggatgtcat
     6841 tatgaaaaag tgcctcacac tactgattgc caccgtcctg agcggaatct ctctcacggc
     6901 ttatgccgcg caaccgatga gtaacctgga cagtggtcaa ctacggcccg ccggtaccgt
     6961 ttcggcaact ggcgcatcaa acctaagcga tctggaggat aaactggcag aaaaagcgcg
     7021 cgaacaaggc gcgaaaggtt atgtcattaa ttccgcaggc ggaaatgacc agatgttcgg
     7081 tactgcaatc atctacaaat aaccccacca acgtaactgc attcgcctct ggatgcagtt
     7141 ttcgtccaat ttgtaagcaa aaaacatcaa atatgaacgc aatgtaaata aatgtatttc
     7201 tttttcgcgc aatgggtgat agaaaatcgc gccaagtgat aatgcttatc aaaattatta
     7261 tcacttgcac ggacgctatc acgggattaa cagtggcatc gcatccgcag agatgctttc
     7321 tcgtgacagt gaaaatttca ccatataaga aaaagtcacc tgcaaaatgg aaaacaatcg
     7381 caatttccct gccagacaat ttcattcgct cacgttcttt gccggtcttt gtattggtat
     7441 tacgcctgtg gcgcaggcac tcgctgccga agggcaagct aacgcggatg acacgctggt
     7501 tgtcgaagca tcaacgcctt cgctttatgc gccacaacaa tctgccgatc cgaaattctc
     7561 gcgtccggta gcggatacta cccgcacgat gacggtgatt tctgaacaag tgattaaaga
     7621 tcagggtgca accaacctta ccgacgcgct caaaaacgtc cccggcgtgg gtgcgttttt
     7681 tgcgggtgag aacggtaact ccaccactgg cgacgccatt tatatgcgcg gtgccgatac
     7741 ctctaacagt atttatatcg atggcattcg cgatatcggc agcgtctcgc gcgacacctt
     7801 caataccgag caggtcgaag taattaaagg gccgtccggc accgactacg ggcgcagcgc
     7861 gccgacaggc tcgatcaata tgatcagcaa acagccgcgc aatgattccg gcattgacgc
     7921 ctccgccagt attggcagcg cctggttccg ccgcggcacg ctggacgtca atcaggtcat
     7981 tggtgatacc actgcggtgc gcctgaatgt aatgggcgaa aaaacgcacg atgccggacg
     8041 cgacaaagtc aaaaatgagc gttacggcgt cgccccttct gtcgcttttg gccttggtac
     8101 agcgaatcgt ttgtatctta attatctgca tgtcacccag cacaacacgc cagacggcgg
     8161 cattccgact atcggtttac cgggttattc tgccccatct gcgggaacag cggctctgaa
     8221 tcattccgga aaagttgata ctcataactt ttacggcacg gattccgatt acgacgattc
     8281 gaccaccgac accgctacca tgcgttttga gcacgacatc aacgataaca ccaccattcg
     8341 caatactacc cgttggtcgc gcgtgaagca ggattacctg atgacggcga ttatgggcgg
     8401 ggcgtcgaat attacccagc ccaccagcga tgtgaatagc tggacctggt cacgcacggc
     8461 gaatactaaa gatgtgagta ataaaattct caccaaccag accaacctga cctcgacgtt
     8521 ctataccggc gctatcggtc atgatgtcag taccggcgtg gaatttaccc gtgaaacgca
     8581 gactaactac ggcgttaatc cggtgacgtt acctgcggta aatatttatc atcctgacag
     8641 cagcattcat cccggcggcc tgacgcgcaa cggcgcaaac gccaatggtc agacggatac
     8701 cttcgcaatt tacgccttcg atacgctgca aatcacccgt gattttgagc tgaacggcgg
     8761 gatccgtctg gataattatc atactgaata tgacagtgcc accgcctgcg gcggcagcgg
     8821 acgcggtgcc atcacctgcc ctgctggcgt ggcaaaaggt tctccggtca ccaccgtcga
     8881 taccgccaag tcgggcaatc tggtgaactg gaaagccggg gcgctgtatc acctgacgga
     8941 aaacggcaat gtctatatta actatgccgt ttcccagcag cctccgggcg gcaacaactt
     9001 cgcccttgcg cagtctggca gcggtaacag tgccaaccgc accgatttta aaccgcaaaa
     9061 agccaacacc agcgagattg gcaccaaatg gcaggttctg gataaacgcc tgttgctcac
     9121 cgccgcgctg ttccgtactg atatcaaaaa tgaagttgag caaaatgatg acggaactta
     9181 ctcgcaatac ggtaagaaac gcgtcgaagg ctatgagata tccgtggccg ggaatatcac
     9241 tcccgcgtgg caggtgattg gcggctatac ccagcaaaaa gcaaccatca aaaacggcaa
     9301 agatgttgcc caggatggtt cctcatcgct gccgtatacc ccggagcacg cctttacctt
     9361 atggagccag tatcaggcaa ccgatgatat ttccgttggt gcgggcgcac gctatatcgg
     9421 cagtatgcat aaaggttctg acggcgcggt gggaacgcca gcgtttaccg aaggttattg
     9481 ggtcgccgac gctaaactgg ggtatcgggt taatcgcaat ctcgacttcc agctaaacgt
     9541 ctacaacctg tttgataccg attacgtcgc ctcaatcaac aagagcggct accgttatca
     9601 cccgggcgag ccaagaacct tcttgctcac ggccaatatg catttctgat tgagatgtgg
     9661 ggcgcaggcc ccactttttg gagaacttgt atgatgtacc acattcccgg cgtgctatcg
     9721 ccacaggacg tcgcccgttt tcgcgaacaa ctggaacaag ccgaatgggt ggatggacgc
     9781 gtcaccactg gcgcacaagg tgcgcaagtt aagaacaatc aacaggtaga cacccgcagc
     9841 acgttatacg ccgccctgca aaatgaggtg ctgaacgcgg ttaaccaaca tgctttgttc
     9901 tttgccgcgg ccttgccgcg taccctttcc acgccgctgt ttaatcgcta tcagaacaat
     9961 gaaacctatg gttttcatgt ggatggcgca gtacgcagtc acccacaaaa cggctggatg
    10021 cgcactgacc tttctgccac gctgttttta agcgatccac aaagctacga tggcggcgaa
    10081 ctggtcgtta atgacacctt cgggcaacat cgggtaaaac ttccggcagg cgatctggtg
    10141 ttgtatccct ccagcagcct gcattgcgtg acacccgtaa cccgcggcgt acgagtggca
    10201 tcatttatgt ggatccagtc gatgatccgc gatgataaaa agcgcgccat gctgtttgaa
    10261 ctggacaaca atattcagtc gctgaaaagc cgctacggcg aaagtgaaga gatcctgtcg
    10321 ctgctcaatc tttatcataa tctgctgcgg gaatggtcgg agatctgacg cttgaactgc
    10381 ccgagttcgt ccctatatta gctacactta actgtacaag tattgatatg gggaggacga
    10441 tatggcatcc ggttgggcta acgatgacgc cgtcaacgaa cagatcaaca gtacaattga
    10501 agatgcgatt gcccgcgctc ggggtgaaat tccgcgtggc gaaagcctgt atgaatgtga
    10561 agagtgcgga gcccccatcc cgcaagcccg tcgggaagcc attcctggtg tgcgcttatg
    10621 cattcattgt cagcaggaga aagatttaca aaaaccagct tatacaggat ataatcgcag
    10681 aggttcgaaa gacagccagt tacgttaact acaactggcg ggatgtatag cgagtataat
    10741 ttgtattttg cgtggcattc cgtgccttta acgctgtcgt gactgccacg ctgcataaaa
    10801 caagtccata agttcaacaa ataaatggat tatcagtatg ttagatatca atcaatttat
    10861 ttgaacaagg cggtcaattc tcttcgattt tatctctcga aaaaaaacgt gatactcatc
    10921 acatcgacga aacaacgtca cttatacaaa aatcacctgc gagagattaa ttatgaaaac
    10981 tatcaatact gttgttgctg ctatggctct ttcaactctg tcatttggcg tgtttgccgc
    11041 ggaaccggta acggcatccc aggcacagaa catgaataaa atcggcgtgg tttctgccga
    11101 tggcgcatcc accctcgatg ccctggaagc gaaactggct gagaaagctg cggcagctgg
    11161 ggcgagtgga tacagcatca cttctgccac caacaacaat aaattaagcg gtactgcggt
    11221 aatttataag taattcgtag aacggataag gcgtttacgc tacgtccagc acaagtattt
    11281 ccctgtctgt ttgccgacag acgcatatgc tctaaccctc attgatccta tgttaccctt
    11341 gtttgcccgt ccgccactgg acgggctttt ttttagctgg ctaactgctg acagaaagcc
    11401 tgcaacgtct cttccggcat accaaccttt cgcgccgcat cacaaatggc ctgccagctt
    11461 cccgcatcca atggaatccc ctgctcctgc cgttcgcgac gcgtgttaac ttcccactcg
    11521 cccggtagca aaatcggctt atcgtcatca tgcggcgagg ctttcaccca ctcggcaaag
    11581 gcttcggtct gcgcgctaca atccggcgcg ccgaacagtt ccgggttgat gatgatagtg
    11641 gtcatgcagt taagaatggc atcgggactg gtttgtaacg ttttctgatg cgtcgtttta
    11701 ccgccggaaa gcgccccacc aagaatttca cacatcgctg caagggcgta gcctttatgt
    11761 tcggcaaagg tcaacagcga acccaacggc gactcctgca ttaccgccgg attggtcgtc
    11821 ggcatgccgt taacgtcaat caggcaacct ggcggcacgg ggacgccttt atgccaggcg
    11881 acgcgggttt tgccaaatgc gatggcgctg gtggcgtaat caagcaacag cgggaaatta
    11941 tctttacgag ggaaaaccac acagaacgga ttggtgccaa agcggctgtc gcgaccgtgg
    12001 aacggcgcga ccattggaat accgaccacg ctaacaaagt ggatagagac aaaccccgcc
    12061 gctgcacact gctccgccca gtaaccgata cggccgatat gatgcgagtt atgtagcgcc
    12121 acggcagcaa taccgtgctg atgcgctttc tcaatcccca gcgccatcgc ttcgtgtgcc
    12181 gcgacctgac caaatgcgcg atcgccatca agcgtaacca ccgcgcccgc ttctttaacg
    12241 attttggcat ggtggttaat ttgcagatgc ccctgactcc aggaacgcac atagcttggg
    12301 atcatgccaa taccatgtga atcatgccct gccaggtttg ccgcgattaa atgatcggca
    12361 actaatttcg cttcttgttc ctcgctaccc atctgacgaa atacagcctg aataaaactg
    12421 tgcagcgtct gagcatcaaa gcgatgacca ctttccatgg tttactcctg tttatgttat
    12481 gtgtttgttg tttttttatg ctgcacgccg ggcgcgggaa ggtcaataaa gatgcaggaa
    12541 tttcatattg cggtggggta aaagccggat gacatggctc atccggctga gataaaaatt
    12601 aaaatgcctg attaatgcgc gccaggccgt cgttgatagt tgccgcttca cccgtagcca
    12661 tcaggcagca agccatctgg attttcagcg attcaggaat cggttcgctg ccagcaaggc
    12721 aacgcttaat ccactgcgcc gtggtttccg gatcttttgc ttgtggcagt aactcgctac
    12781 cagcagtgtc ctgtttttca tacagtaccc gcatcccttc acggtcaatg agattgatct
    12841 gcgggcagcg ctgcggatta gcatacactt caccttcagt accatgcatt agtagcgccc
    12901 gcccgccgat atcactaaag aacttcgcga cgcgtccaat gtattccgga tgcgaaacgc
    12961 tggaaagacg cagtgcctca ccttcggcaa atggcgtcgc cagtttcgcc agggtatgtg
    13021 cactgttacg cacgcccatc cgccagcgca tcgccagttg tttttccagc ggcgggcaaa
    13081 acgcgccgac aggcataaac accggttgat gttcgtcgag cttcgcctgc gcctgcccgc
    13141 cgtgcaacgt tggcgtaata cccatcagtt cgaaaatggt ttctgtcagc acgcgggttg
    13201 gatcttcgct aaccccgtga accaccacag gaaaaccgag tttgtgcagg agaatcgcca
    13261 acaacggcgt caggttggcc tgtttacgcg cgccgttgta actggggatg acaatcggca
    13321 tcggcttgtc tgcaggcggc gtcagcttga tggtgtgatt ttgcatggct tcgtaaaagc
    13381 cgagcatctc tgcttcccct tcccctttga tacgcagcgc aatcaatacg ccgcccaact
    13441 caaggtcagg gacttcacca ttgagcatat gggcatacag accgcgcgca gtatcccggt
    13501 ctaaatcgcg cgcgtggttt ttcccgcgcc cgatctcttt aatgattttg cgatagtcca
    13561 tttacgactc cttacctgac tcacatcatt aacgccgccg acggcgtggg gatttcgttt
    13621 tttctttctt tttaactata ccttcaggga cttccggttg ctctatcgga aataccggta
    13681 atgcatccag tagtcgcttg ccataatttt tggtcagcaa gcgtttgtcg tagataacca
    13741 cttcccccca gcaaccgtgg cttcgaatca gtcgcccaac ctgctgaatc aggttaaacg
    13801 aagcgctcgg caggctttgc acctcaaacg gatagcggtt gaggcttttc agccattcac
    13861 cttcggtgat caccaccgga ctgtcgatgg gcggaaaagc gatcttgtgg atatgcacct
    13921 gactgagcag atcgcctttc agatcaagac cttcggcaaa ggactgtaag cccaccagca
    13981 cgctacgctc accgttggca acgcgtttgc ggtgcagttc aactaagcga taacgtggct
    14041 gatcgccctg caccagcaac atcaggcgta aatccgtcac atagtcgaga aagcgctgca
    14101 tcgcacgccc gctggcaaac agtaccaaca taccgagatg ttttttgctc tccacctgct
    14161 cacggaaaaa ggctgccatt tcagcaatgt gctgctcttc gttgtcgatg gaaggctcaa
    14221 agcgcatccg gggaataaca attttgccct gttcgcagtg gttaaagggc gaatccagcg
    14281 ccacaaagcg gtcgcccgct ttctctttca gaccactcat ctcctgtaaa cgcgaaaaac
    14341 tgttcagcga acgtaaggtc gcggaagtga caataatgtg cggaatactg cgccacagca
    14401 gcctttccag ctgatcgctg acacgaatac ccacacagtg aaaccagaga tgcagctgcc
    14461 cttcgcgctc ttcccgcgtc gcccatttag tcaccggcgc accggaagat tgcgccagag
    14521 acgccagccg ccagagtttg ctttgcgcct cgaacatccc caacgcgcga ttcatctgca
    14581 aaatcaaccg atgcagacgt acaatgtcat ggctaccggt tttctcactt aaatcgttaa
    14641 gaaataactc cgccaggcca cgcagcatct cggtgagttt tgccagccgc tggcagatct
    14701 ccagcacttc atcaggcagt tcgcccatcg caaaacggtg ctctgcctcc tgcccggcag
    14761 gcatgtagag attgagaatg ttgtttaacg aggcgataag ctcatacagc tcttcacaat
    14821 gcgcattcaa acgttcaggg atcgccagcg gtgggatggt cttcgggcga aactgctcca
    14881 tgcaggtagc gaccagtttc gtgaacaagt ccagctgtag ccgataccag ggcgcggtga
    14941 tttcggcgct catctccagc gcatcccgcg ccacatctgg caggtgatga ccttcgtcca
    15001 gcaccagcag cagatttttc gggtcaggta gtacggcttc gctttccatc gccgccatca
    15061 ccagcgcatg gtttgccacc accacttccg cttcctgaat ctcccgacga gcaacaaaaa
    15121 acgggcattc acgatagtag taacagttgc ggttgaggca gctggctttg tcggtactta
    15181 aacgacgcca gagatcgtca tcaatggcga tatccgtatg atcgcgcaga ccatcccatt
    15241 tataagtgtc gagatcgccc ttcagcttcg cacaacgttt ttgctcttcc tgattgttcg
    15301 gcgtcagctc gtcgtcaaga aacgccagca gatcctgttg cgtgggttca gtactggcaa
    15361 gcgctgtcag attacgcgga caaacgtagc gcccacgccc aaaagcagca gtgaatttaa
    15421 gatcgggaat gatctttttc agcagaggca aatctttact gtagatctga tcctgcaatg
    15481 ccacgttggc ggtactcacc accagcgttt tttgctcttc gcgggcaatg gcgatgccgg
    15541 gaatcaaata ggagagcgtt ttcccaacgc cggtgggtgc ttcaatcgcc agatgccgcc
    15601 cttcttctcc ggccagcgtt ttggcgacgt ccgcaatcat ctgccgctgc ggcgcacggg
    15661 gaataaagtc ggggatctgt tcctgaagcg ccttatacca ggcggcaatt tgcgctttaa
    15721 gcgcggcggt taatgccatg agaaaacctg aaatactgta taaacagcca atattgtggc
    15781 atttttcggt tttcaggggt aatggttttt ctgtttctgg aaagcggctc gcaataacca
    15841 gcaggatgat gcgtaaagcg cttcgcgcta aattcgaaca acatgcagaa ctgcgtacac
    15901 tcttgctggc aaccgcgtcc gcaaaactgg ttgagcatac gcaaaatgat gcttactggg
    15961 gagatagcgg taatggtcag ggcaagaatc gcctggggta ccttttaatg gcgttgcgcg
    16021 gacaattggc tgcggagaag taacgcatcg ggtggcttgt gggcgttaaa acagagaaat
    16081 ctttttgccg cgctcgcaaa tggctgacaa gtgcttgttg tgaaatgccg gatgcggcgt
    16141 aaacgcctta tccggcctac aaaagcatgc aaattcaata tattacagag ttcacgtagg
    16201 cctgataagc gcagcgcatc aggcagtttt gcgtttgttc atcagcctga tgccgggcat
    16261 cgcccggcat caaaagatta ctgcgcagcg gcaggtttac gtggacggcg gcgacgttgt
    16321 tgttcgcctg ccggtttggc atcgccgagg cggcgagacg gtttttctgc aggtttcgca
    16381 cttgcagatt ttgcgccacc ttctccacgg cgtggttgct gttgaccgcg accaccacct
    16441 tgcccacgac cgccgccacg ttgctggcga ccgttctgga tcggttcggc tttgattgac
    16501 gggtccggct cataacccgg aatcgcaatg cgcgggatct cttttttcag cagtttttcg
    16561 atatcacgca gcagtttgtg ttcatcaaca cacaccagcg acaacgcttc accggtagca
    16621 gccgcacgac cggtacgccc gatacggtgg acataatctt ccggtacgtt tggcagttca
    16681 tagttgacca cgtgcggcag ctcttcaata tccaggccgc gcgcagcgat gtcagttgcc
    16741 accagtacac gaatatcgcc cgatttaaaa tcagccagcg cacgagtacg cgcaccttgc
    16801 gatttattac cgtggatcgc cgcactacgg atgccatctt tattaagctg ttcagccaga
    16861 tggttagcgc catgtttggt acgggtaaac accagcacct gctgccagtt ccctttccca
    16921 atcatatgcg acagcaattc gcgtttgcgt ttcttatcga caaagtgaac gtgctgggtc
    16981 acctgatcag acgcggtatt gcggcgtgcc acttcgattt ccagcgggtt gtgcaacagt
    17041 ttttccgcca gggctttaat atcgtcagag aaggtcgcgg agaacaacag gttctggcgc
    17101 ttcgctggca gttttgttaa cacgcgacgg atatcgtgga taaagcccat gtcgagcatg
    17161 cggtcagctt catcaaggac gaggatttca acctgatcca gcttcactgc attttgatgt
    17221 tccaggtcca gcagacgtcc cggcgttgcc accagcacat caacgccgcc gcgcaacttc
    17281 atcatttgtg ggttaatgct gacaccgcca aacaccacca gcgaacgaat gttcagatat
    17341 ttgctgtaat cacggacgtt ttcgccaatc tgcgccgcca gttcacgggt cggggtaaga
    17401 atgagcgcgc gtaccggacg acgccctttg gcgtgcggct ggcgagtgat caggtgttgc
    17461 aacagcggca gcgtaaagcc cgctgttttg ccggtaccgg tctgggcgct ggccatcagg
    17521 tcgcggcctt ccagcaccgc agggatcgcc tgctgctgaa taggggtggg ttcacggtaa
    17581 ccctgctcgg caacggcgcg caggatatca gggcttaaac ccaaagaatc gaaagacata
    17641 actactccga accgccccga ccgttaccgg tgtagttttc agggagatac tgaaaaggaa
    17701 aatgacaaaa accacaatgt catgagggag cggagtgtag cagtttttgt gacgcagcgc
    17761 ataaattatc cctgtgactg gcgttgctaa aattcttagc catactggac aactctcata
    17821 tggagtcata atcttaatca atcatttgat taagaatgga ctgtgcgatg aataatcctg
    17881 ccatgacaat caagggtgaa caggcgaaga aacagctgat tgctgccgca ctggcgcagt
    17941 ttggtgaata tggaatgaac gccaccaccc gcgagatagc cgcccaggcc gggcagaata
    18001 tcgctgccat cacctattac ttcggttcga aagaggattt gtacctcgcc tgcgcccagt
    18061 ggattgccga ttttattggc gagcagttcc gtccacacgc cgaagaagcc gaacgtttgt
    18121 tcgcgcaacc acagcctgat cgcgcagcta tccgtgaact gatccttcgc gcctgcagga
    18181 acatgattaa gctgctcacc caggatgaca ccgtcaacct cagcaaattt atctcccgtg
    18241 agcagctctc tcccacggca gcctatcacc tggtgcacga acaggtgatt agtccgctac
    18301 acagccacct tacacgcctg attgccgcct ggaccggctg cgacgctagc gatacccgca
    18361 tgatccttca tacccatgcg ttgattggtg agattctggc gtttcgtctt ggcaaagaaa
    18421 ctattctgtt acgtaccggc tggaccgcgt tcgatgaaga aaagaccgaa ctgatcaacc
    18481 agacggtgac ctgtcatatt gacctgattt tgcaaggatt atcgcaaagg agtttgtagt
    18541 gatgaaaaaa cctgtcgtga tcggactggc ggtagtggta cttgccgccg tggttgccgg
    18601 aggctactgg tggtatcaaa gccgccagga taacggcctg acgctgtatg gcaacgtgga
    18661 tattcgtacg gtgaatctta gtttccgtgt tggggggcgc gttgagtcgc tggcggtgga
    18721 cgaaggtgat gcgatcaaag cgggccaggt actgggcgaa ctggatcaca agccgtatga
    18781 gattgccctg atgcaggcga aagcgggcgt ttcggtggct caggcgcagt atgacctgat
    18841 gcttgccggg tatcgagatg aagaaatcgc tcaggccgcc gcagcggtga aacaggcgca
    18901 agctgcctat gactatgcgc agaacttcta taaccgccag caaggactgt ggaaaagccg
    18961 caccatttcg gcaaatgacc tggaaaatgc ccgctcctcg cgcgaccagg cgcaggccac
    19021 gctgaaatca gcgcaggata aattgcgtca gtaccgttcc ggtaaccgcg aacaggacat
    19081 cgcccaggcg aaagccagcc tcgaacaagc gcaggcgcaa ctggcgcagg cggagctgaa
    19141 tttacaggac tcaacgttga tagccccgtc tgatggcacg ctgttaacgc gcgcggtgga
    19201 gcctggcacg gtgctcaatg aaggtggcac agtctttacc gtctcactga cacgtccggt
    19261 gtgggtacgt gcttatgttg atgaacgtaa tctcgaccag gcccagccgg ggcgcaaagt
    19321 gctgctttat accgacggtc gcccggacaa accgtatcac gggcagattg gtttcgtttc
    19381 gcctaccgct gaatttaccc cgaaaactgt cgaaacgccg gatctgcgta ccgacctcgt
    19441 ctatcgcctg cgtattgtgg tgaccgacgc cgatgatgcc ttacgccagg gaatgccggt
    19501 gacggtgcaa ttcggtaacg aggcaggaca tgaatgatgc cgttattacg ctgaacggtc
    19561 tggaaaaacg ctttccgggc atggacaagc ccgccgtcgc gccgctcgac tgtaccattc
    19621 atgctggtta tgtaacgggg ctggtggggc cggatggcgc aggtaaaacc acactgatgc
    19681 gcatgctggc gggattactg aaacccgata gcggcagcgc cacggtgatt ggctttgatc
    19741 cgatcaaaaa cgacggcgcg ctgcacgccg tgctcggcta tatgccgcag aaattcggcc
    19801 tgtatgaaga tctgacggtg atggagaacc tcaatctgta cgcggatttg cgcagcgtca
    19861 ccggtgaagc gcggaagcag acttttgctc gcctgctgga atttacatct cttggcccgt
    19921 ttaccggacg cctggcgggc aagctctccg gcggaatgaa gcaaaaactt ggcctggcct
    19981 gtaccctggt gggcgaaccg aaagtgttgc tgctcgatga acccggcgtc ggcgttgacc
    20041 ctatctcacg ccgcgaactg tggcagatgg tgcatgagct ggcaggcgaa ggaatgttaa
    20101 ttctctggag cacctcgtat ctcgacgaag ccgaacagtg ccgtgacgtg ttactgatga
    20161 acgaaggcga gctgctgtat cagggagaac cgacggccct gactcaaact atggccggac
    20221 gcagctttct gatgaccagc ccgcacgagg gcaaccgcaa actgttgcaa cgggcattga
    20281 aactgccgca ggtcagcgac ggcatgattc aggggaaatc ggtacgtctg atcctcaaaa
    20341 aagagaccac gccagacgat attcgccatg ccgacgggat gccggaaatc aacatcaacg
    20401 aaactacgcc gcgttttgaa gatgcgttta ttgatttgct gggcggtgcc ggaacctcgg
    20461 aatcgccgct gggcgcaata ttacatacgg tagaaggcac acccggcgag acggtgatcg
    20521 aagcgaaaga gctgaccaaa aagtttggcg attttgccgc caccgatcac gtcaactttg
    20581 ccgtcaaacg cggggagatt tttggtttgc tggggccaaa cggcgcgggt aaatcgacca
    20641 cctttaagat gatgtgcggt ttgctggtgc cgacctctgg tcaggcgctg gtgctgggca
    20701 tggatctgaa agagagttcc ggtaaagcgc gccaacatct cggctatatg gcgcaaaaat
    20761 tttcgctcta tggcaacctg acggtcgaac agaatttacg ctttttctct ggtgtgtatg
    20821 gcttacgcgg tcgggcgcag aacgaaaaaa tctcccgtat gagcgaggcg ttcggcctga
    20881 aaagtatcgc ctcccacgcg accgatgaac tgccattagg ttttaaacag cggctggcgc
    20941 tggcctgttc actgatgcat gaaccggaca ttctgtttct cgacgaaccg acgtccggcg
    21001 ttgatcccct cacccgccgt gaattttggc tacatatcaa cagtatggta gagaaaggcg
    21061 tcacggtgat ggtcaccacc cactttatgg atgaagcgga atattgcgac cgcatcggcc
    21121 tggtgtaccg cgggaaatta atcgccagcg gcacaccgga cgatttgaaa gcgcagtcgg
    21181 ccaacgatga gcaacccgat cccacgatgg agcaagcctt tattcagttg atccacgact
    21241 gggataagga gcatagcaat gagtaatccg atcctgtcct ggcgtcgcgt gcgggcgctg
    21301 tgcgtcaaag agacgcggca gatcgttcgc gatccgagta gctggctgat tgcggtagtg
    21361 atcccactgc tactgctgtt tatttttgga tacggcatta acctcgactc cagcaagctg
    21421 cgggtcggga ttttactgga acagcgtagc gaagcggcgc tggatttcac ccacaccatg
    21481 accggttcgc cctacatcga cgccaccatc agcgataacc gtcaggaact gatcgccaaa
    21541 atgcaggcgg ggaaaattcg cggtctggtg gttattccgg tggatttcgc cgaacagatg
    21601 gagcgcgcca acgccaccgc accgattcag gtgatcaccg acggcagtga gccgaatacc
    21661 gctaactttg tgcaggggta tgtcgaaggg atctggcaga tctggcaaat gcagcgagcg
    21721 gaggataacg ggcagacttt tgaaccgctt attgatgtac aaacccgcta ctggtttaac
    21781 ccggcggcaa taagccagca ctttattatc cccggtgcgg tgaccattat catgacggtc
    21841 atcggcgcga ttctcacctc gctggtggtg gcgcgagaat gggaacgcgg caccatggag
    21901 gcgttactct ctacagagat tacccgcacg gaactgctgc tgtgtaaact gatcccttat
    21961 tactttctcg ggatgctggc gatgttgctg tgtatgctgg tgtcagtgtt tattctcggc
    22021 gtgccgtatc gcggttcgct gctgattctg ttttttatct ccagcctgtt tttactcagt
    22081 accctgggga tggggctgct gatttccacg ataacccgca accagttcaa tgccgctcag
    22141 gtcgctctga acgcggcttt tctgccgtcg attatgcttt ccggctttat ttttcaaatc
    22201 gacagtatgc ccgcggtgat ccgcgcggtg acgtacatta ttcccgctcg ttatttcgtc
    22261 agcaccctgc aaagcctgtt cctcgccggg aatattccag tggtgctggt ggtaaacgtg
    22321 ctgtttttga tcgcttcggc ggtgatgttt atcggcctga cgtggctgaa aaccaaacgt
    22381 cggctggatt agggagaaga gcatgtttca tcgcttatgg acgttaatcc gcaaagagtt
    22441 gcagtcgctg ctgcgcgaac cgcaaacccg tgcgattctt attttacccg tgctgattca
    22501 ggtgatcctg ttcccgttcg ccgccacgct ggaagtgacc aacgccacca tcgccatcta
    22561 cgatgaagat aacggcgaac actcggtaga acttactcaa cgttttgccc gcgccagcgc
    22621 ctttacccat gtgctgctgt tgaaaagccc gcaggagatc cgcccaacca tcgacacaca
    22681 aaaggcatta ctcctggtgc gtttcccggc tgacttctcg cgcaaactgg ataccttcca
    22741 gaccgcgccg ttgcagttga tcctcgacgg gcgtaactcc aacagtgcgc aaattgccgc
    22801 caactacctg caacagatcg tcaaaaatta tcagcaggag ctgctggaag gaaaaccgaa
    22861 acctaacaac agcgagctgg tggtacgcaa ctggtataac ccgaatctcg actacaaatg
    22921 gtttgtggtg ccgtcgctga tcgccatgat caccactatc ggcgtaatga tcgtcacttc
    22981 actttctgtc gcccgcgaac gtgaacaagg tacgctcgat cagttactgg tctcaccgct
    23041 caccacctgg cagatcttca tcggcaaggc cgtaccggca ttaattgtcg ccaccttcca
    23101 ggccaccatt gtgctggcga ttggtatctg ggcgtatcaa atccccttcg ccggatcgct
    23161 ggcgctgttc tactttacga tggtgattta cggtttatcg ctggtgggat tcggtctgtt
    23221 gatttcatca ctctgttcaa cacaacagca ggcgtttatc ggcgtgtttg tctttatgat
    23281 gcccgccatt ctcctttccg gttacgtttc gccggtggaa aacatgccag tatggctgca
    23341 aaacctgacg tggattaacc ctattcgcca ctttacggac attaccaagc agatttattt
    23401 gaaagatgcg agtctggata ttgtgtggaa tagtttgtgg ccgctactgg tgataacggc
    23461 cacgacaggg tcagcggcgt acgcgatgtt tagacgtaag gtgatgtaac ttcttatctt
    23521 tcgccagcaa agacactacc gccgggccag caaggattgc cagccctgca atcgccagca
    23581 aaaagttgtt ttccagtacg cgggacaaca gccacagcac aatcagcgct gcgccaaaat
    23641 accaggtcgt ggtcagctct tccagcacgt ctgccacctc acgccagcgt tgctcggggt
    23701 ggcgctgaaa caccagcatc atcactaccg tgacacaata cagtgcgcat aatcccacgc
    23761 cgacgtgaac cagagtctta ctcatccagc ccaccaccag cagcgccact accagtaaat
    23821 gcaacataat ctgccagcaa cttagacccg ttgcgacacg aacacgttgt tgccacttca
    23881 tggcttctct cctgaagaat ttatctctac ttactcagag taccgaactt tacggaaaat
    23941 tccgtaagac cgcacctttt tgtgacgccg actacactat ttcaatgaga taagaggcaa
    24001 agacaggaaa attatgcccg atcaaacaca acaattttcg ttcaaggtgc tcaccatcaa
    24061 tattcacaaa ggctttaccg cgtttaaccg acgcttcatt ttgccggaac ttcgcgacgc
    24121 cgtgcgtacc gtcagcgccg atattgtttg cctgcaggaa gtgatgggcg cgcacgaagt
    24181 tcatccgctg catgtggaaa actggcccga tacctcgcac tacgagtttc tcgccgacac
    24241 catgtggagc gatttcgcct acggtcgcaa tgccgtatac ccggaagggc atcacggcaa
    24301 cgcggttctt tcacgttatc ccattgaaca ttatgagaat cgcgatgttt cggtcgatgg
    24361 tgcggaaaag cgcggcgtgc tctactgccg cattgtgccg ccgatgaccg ggaaagcgat
    24421 tcatgtgatg tgcgtacatc tgggcctgcg tgaggcgcac cgtcaggcgc agcttgcgat
    24481 gctcgccgaa tgggtgaatg agctgccgga cggcgaacca gtattggtgg cgggtgattt
    24541 caacgactgg cggcaaaaag ctaatcatcc gttaaaaatg caggccgggc tggatgagat
    24601 atttacccgc gcccacggac gcccggcgcg cacgtttccg gtgcaatttc ctctactacg
    24661 tctggacagg atctatgtca aaaatgccag cgccagcgcg ccaaccgcgt tgccgctgcg
    24721 gacatggcga cacctttctg atcatgcccc tttaagtgcg gagattcatt tatgaaatgt
    24781 agctggcgcg aaggcaataa gatccagttg ctggaaaacg gcgagcaata ttatcccgcg
    24841 atgtttaagg cgattggcga ggcgcaagaa cgcatcattc ttgaaacgtt tatctggttt
    24901 gaggatgacg tcggcaaaca gctgcatgcg gcgctactgg cagcagcgca acgcggggtg
    24961 aaagcggaag tcttgctgga tggctacggt tcgccggatc tcagcgatga gtttgtcaat
    25021 gaactgacgg cagctggcgt ggtatttcgc tactacgatc cccgtcctcg cctttttggt
    25081 atgcgcacca atgtgtttcg ccggatgcat cgcaaaattg tggtgatcga cgcgcgtatc
    25141 gcctttattg gcgggctgaa ttactccgcc gagcatatgt ccagctacgg tccagaggct
    25201 aaacaggatt acgcggtacg ccttgaaggg ccgattgtcg aagatattct ccagtttgag
    25261 ctggaaaacc tgcctggaca gagcgcggca cgacgctggt ggcgacgtca tcacaaagcg
    25321 gaagagaacc gccagccggg agaagcgcag gtattgctgg tctggcgcga taacgaagaa
    25381 catcgcgatg atattgaacg tcactatctg aaaatgctca ctcaggcgcg gcgagaagtc
    25441 attatcgcca acgcctactt cttccccggc tatcgatttt tacacgcctt gcgtaaagcg
    25501 gcacggcgcg gggtgcggat caaactgatc attcagggcg aaccggatat gccgattgtc
    25561 agagtcggtg cgcgcttgct gtataactat ctggttaaag gcggcgttca ggtatttgag
    25621 taccgccgcc gcccgctcca cggcaaagtg gcattgatgg acgatcactg ggcgacagta
    25681 gggtccagta atctcgatcc gctcagtttg tcactgaatc tcgaagcaaa tgtcatcatc
    25741 cacgatcgtc attttaacca gacgctgcgc gataatctga acggcattat tgccgcagat
    25801 tgtcagcagg tcgatgagac catgctgccg aaacgcacct ggtggaacct gaccaaaagc
    25861 gtgctggcgt tccacttttt acgccacttc ccggcgctgg ttggctggct tccggcacat
    25921 acaccacgtc tggcgcaggt tgatcccccc gcacaaccga caatggaaac gcaggatcgg
    25981 gtagaaactg aaaacacggg ggtaaaaccc tgatgagtaa atcacacccg cgctggcgct
    26041 tagcaaagaa gatcctcacc tggctgtttt ttatcgcggt gattgtgttg ctggtggtct
    26101 acgccaaaaa agtggactgg gaagaggtct ggaaggtcat ccgcgactac aatcgcgttg
    26161 cgctgctcag tgcggtcggg ctggtggtcg tcagctatct gatttacggc tgctatgacc
    26221 tgctcgcccg tttctactgc ggtcacaaac tggcgaagcg ccaggtgatg ctggtgtcgt
    26281 ttatctgcta cgccttcaac ctgacgctca gtacctgggt cggcggcata ggtatgcgct
    26341 atcgtttgta ctcgcggcta gggttaccgg gcagcactat tacgcggatt ttctcgctca
    26401 gtattaccac caactggctg ggctatattt tgctggccgg gattatcttt accgcaggcg
    26461 tggtggagct gccggatcac tggtatgtcg atcaaaccac gctgcgtatt ctcggcattg
    26521 gcttattgat gattatcgcg gtttatttgt ggttttgcgc tttcgcgaag caccgccata
    26581 tgaccatcaa agggcaaaag ctggtgctgc cttcatggaa attcgccctc gcccagatgc
    26641 tgatttccag cgttaactgg atggtcatgg gagcgataat ctggctgtta cttggtcaaa
    26701 gcgtgaacta tttctttgta ctgggcgtgt tgctggttag tagtattgcc ggggtgattg
    26761 tgcatattcc ggcggggatt ggtgtgctgg aagcggtgtt tatcgcacta ctggctgggg
    26821 agcatacctc caaaggtagc attatcgccg ccctactcgc ttaccgtgtg ctgtattact
    26881 ttatcccact gctgctggcg ctgatctgtt acctgctact ggaaagccag gcgaagaagc
    26941 tgcgggcgaa aaatgaagcg gcgatgtgaa gttcggttaa gttctttagt atgtgcattt
    27001 acgactaatg aaaaaaccgc gtatgccttt gccagacaag cgttatagct atcgcggcaa
    27061 gtatcccaaa gaagccgata aaatagtaat agagtgttaa tgcacctgta aaagcgcaac
    27121 taaccggact ccacaactct tcgctatggc attttcctgc cagttcaagg agcttatttc
    27181 gtccatgcga gataatgcct gaccataaaa caatcattaa gcaacttatt acccaaatgg
    27241 gccgttcact cattaatgcg gcattcctga tgatcaccag ggccagtcct gcggttgtca
    27301 ccaaacccaa agcggtaatg agccatatgc cgtttaatat cctggcttcg tgctgaaaat
    27361 ctaatttatt gctgctttgc gagtatgatt ccattctttt agttatcctt agaaaaaata
    27421 ttaatattca ccatcaataa atttaaagcc acgcatacaa aatacttttg tatttatgga
    27481 tacggaaaac acttccgctc ggccataagg catattttat acttggtact tgttaagtgt
    27541 cgttttttcg taagaaaaaa atcccgacac atggccggga tattgatggt gaaaaagaat
    27601 taacggcggt tgccgaagat ccgcaacaac atcaggaaca agttgatgaa gtccagatac
    27661 aaggttaacg cgccaagaat ggaatatttg cgcaggttcg acgtgtcgcg ggtatcaatc
    27721 tgctcaccca tatttttcaa tttctgcgtg tcatacgccg tcaatccgac aaagacaatc
    27781 acgccaatgt aggtaactgc ccacatcaat gcttcgcttt tcagccagaa gttgaccagc
    27841 gatgccagca caatgccgat taacgccata aacagcatat tgccaaagcc gcttagatcg
    27901 cgcttcgtgg tgtaaccgta caggctcatt gcgccaaaca ttccggcagt aacgacgaaa
    27961 gtactggcga tagaagcagc ggtataaaca atgaaaatac tggaaagcgt aagacccgtc
    28021 agcgccgaat aaagcataaa gagcatcgtc gttacacctg cgctcagctt ttgaatcatc
    28081 gctgataaca caataaccaa tgctaattgc gcgatgatca gaccgattaa aaagacacgg
    28141 ttagtgaaca acagctccat cacagccgcg gaattagccg cataccaggc aacaaatgcg
    28201 gtcagcaaca agccaacggt catccagcca tagacttgcg ccatataagt ttgcaagcca
    28261 gcccggggtt gtacaattga atcagaacgt gggaatctgt ccatgacgat ctcctgaaga
    28321 tataaggaat atcttaagga tactgcaaaa tgatgaggct gtgcatcgac gcagcgtaaa
    28381 cgcatgtact gagcggtgaa attgccggac gcagcggtgc cttatccggc taacaaaaaa
    28441 ctaccagcgt tttgccgcct gctgatcgct ctcccgagct tcaacccagc ggtcgccttc
    28501 cggcgtggct tcacgcttcc agaacggtgc gcgggttttg agataatcca taataaactg
    28561 cccggcttca aacgcactgc tgcgatgcgc actggtgaca ccgacaaaaa cgatttcatc
    28621 tcccggccat aattccccga tgcggtgaat caccgtgacg cgccccagcg gccagcggtt
    28681 acgcgcttca tcgacaattt ctgccagtgc tttttcagtc atccccggat agtgttcgag
    28741 ggttaatgct ttgacgctgt cgccaaggtt atggttgcgc accttaccgg taaaggtgac
    28801 taccgcaccg tcttcatcac gctccgccag ccacggatac tcttctccta cgctgaatgg
    28861 ctgcgggccg acaacaattt tggtttctgc catcttaacc tccggttacc ggcgggaaga
    28921 aagccacttc gtcgccgtca gtcagcggat ggtcaagact caccagcgtc tggttgacgg
    28981 cagccagcaa tttgccatct tccagcgcca gcgcccagcg atcgctctgc gcagccagat
    29041 gttggcgtaa cgcttcaacg gttgggaaat ccgcagccac ttcggttgca tctgttccca
    29101 ccaactcgcg cacctgggcg aaaaaaagaa ctttaatcat ccgcttccac cttaaagtca
    29161 cccgacttgc cgccgctctt cgccagcaaa cgtaccggac caattaccat atctttttgc
    29221 accgctttgc acatgtcata aatggtcagc gccgccacgg aggccgcggt taacgcttcc
    29281 atttcgacac cggttttccc ggtcaggcgg cataaggttt ctatacgtac ccgattgtgc
    29341 tccggctcgg cctgtaaatt gacttcaact ttgctgagca tcagcgggtg acacagcggg
    29401 atcagatccc aggtgcgttt tgccgcctga ataccggcga tacgcgcagt ggcaaatacg
    29461 tcgcctttgt ggtggcgacc atcaataatc atcgccagcg tctcgctgcg catggtgaca
    29521 aaggcttccg cccgcgcttc acgcacggtt tccgctttgg cggagacatc caccatgtgt
    29581 gcttcgccag cggcgttgat atgggtcagt tgcgacatac ttatttcttc aaatgtggat
    29641 ggaaattgca tggacgtgta cgggcatcga gctgcggcgc gatgatattt tcccacgcgg
    29701 tgcggcacgc tttggtcgaa cccggcatgg cgaaaatcag cgttttgtta gcgacgcccg
    29761 ctaccgcacg agattgcaac gttgaggtgc caatctcttc aaacgacaac atccggaaca
    29821 cttcaccaaa accttcaact tcacggtcga acaacggcag cagcgcttcg ggagcctgat
    29881 caccttccgt caggccagta ccacccgtaa tcaataccac ttgtacatcg tcgctggcga
    29941 tccacgcaga tacctgagcg cgaatagcgt agcggttttc tttcacaatg gctttatcga
    30001 caacgtgatg gcccgcttcc tgcgccgaat cgcgcagata gtgaccggag gtatcgtctt
    30061 cttcaccacg acgattagaa accgtaagaa tagcaatacg ggtcgggata aattcagtgc
    30121 ttacctgact catctgatct ctccttttga cgttttagcc gccaatgtac gataagtttt
    30181 gcgtaatacc ggtgttgttt tgatgcagga aatgggtctg tttcttctcc cgcagcgccg
    30241 ctgaaatacg cgcttccagc gcctgttgct gggtatcgtc ttccagcaga tcgcgcaggt
    30301 taacgccgcc ttcaccaaac aggcagagat ggagtttacc aatggaggaa acgcgcaggc
    30361 ggttgcaagt ggcgcagaag tctttttcat acggcatgat aaggccaatc tctccggcgt
    30421 aatccggatg acaaaagact tgcgcgggac cgtcgctgcg ttgacgtaat tggtggatcc
    30481 agccgcgacg cagtagctcg tcacgcagaa cctgaccaga gatgtgatgc ttacggaaga
    30541 gctcgctgcc ctcacccgtt tccatcaatt cgatgaaacg cagctggata gggcgatgct
    30601 ggatccagtt cagaaaggtg tcgagctgat gatgattaac atcacgcatc agcacggtat
    30661 tgactttgac cttctcaaaa ccggcctcaa atgcagcatc gatccctgcc atgacctggt
    30721 tgaatttatc ctgcccggta atggcatgaa actggcgagc gtcaaggcta tcaacgctga
    30781 cgttgatgcc agtaagtccc gcatcgcgcc agttcgccac atcgcgttcc agacggtaac
    30841 cattggtggt gaccgcaatc tggcggatag cgtcgttttc ccgcacagcg gcgatgatat
    30901 cggtaaagtc gcggcgtaaa gacggctcac cgcctgtcag gcgcactttt tcggtgccca
    30961 gactggcaaa ggcgcgcgta acccggcgaa tttcatcgac ggtaagaaag cctttattgg
    31021 tgacgccgct cggtttgtag ccatccggca ggcagtaggt gcaacgaaag ttacacacat
    31081 cagtaatcga caggcgcaag tagtaaaact tacgcgcaaa tgcatcagtc agttgtgaag
    31141 ccatgtacac ctttccagat acgggaggcg aagtcatttc ttccttcgcc ctggtggcaa
    31201 tctttacgag cattgtcacg gccaaagcac cgtatcagtt gacccaggtg cagaggctag
    31261 agtgtttagt ggttaagccg atactagcgt gtaaatgaga tttttaccat ccacactttc
    31321 gctatgtaat catgtatata gcgtcatgat cgtgctattt cgctacagaa taagggaaaa
    31381 tggcggtttt actttgatat acatcatcag gaaggcatag cgtacatcgt cctttagagg
    31441 tagaagaaaa tagtttgtat cctcaatatt ggcaggttaa ttgctgtttc cccgcaattt
    31501 gcgctactgt agcgcggata atctgactcc aggaaactat atgcgcaatc gtacgctggc
    31561 tgaccttgat cgtgtcgttg ctctcggcgg agggcatgga ctgggacgcg ttctctcatc
    31621 actttcgtct ttgggttctc gtttaacggg tatcgtcacc accacggata atggcggatc
    31681 gacagggcgt attcgccgct cagaaggcgg tattgcctgg ggcgatatgc gtaactgcct
    31741 caaccagctg ataactgaac ccagcgtcgc ttccgcaatg tttgaatatc gtttcggcgg
    31801 taatggcgaa ctttccgggc acaaccttgg aaatctgatg ctgaaggcac tggatcacct
    31861 tagcgtgcga cctcttgaag caatcaatct gattcgcaat ctgctgaaag tggacgcaca
    31921 tttgattccg atgtcggagc atccggtgga tctgatggcg attgacgatc aggggcatga
    31981 agtttacggc gaggtcaata tcgaccagtt aactacgccg attcaggagt tactgttaac
    32041 gccgaatgta cccgcaacgc gtgaggctgt tcacgctatc agtgaagcgg atctcatcat
    32101 tattgggcct ggcagttttt acaccagcct gatgccaatt ctgctgctga aggaaatcgc
    32161 acaggcatta cgccgcacgc cagcgccgat ggtctatata ggcaatctgg ggcgtgagtt
    32221 gagtttacct gcggctaatt tgaagctgga aagcaagctg gcaattatgg agcagtatgt
    32281 cggtaaaaag gtcattgatg cggtcatcgt cgggccaaaa gtggatgtct cggcggtgaa
    32341 agagcggatt gtgattcagg aggtactgga ggccagcgat attccgtatc gtcatgaccg
    32401 ccagttgtta catagcgcgc tggaaaaggc attacaggct ttaggttaac ggaaagccga
    32461 aggaaaattc cggcttccta ttgaagacaa agtgcgcgtt gtttatgccg gatgcggcgt
    32521 gaaagcctta tccggcctac aaaccgcgct aattcaatgt attgcagaga tcatgtaggc
    32581 ctgataagcg tagcacatca ggcaattttg cttttgtcat ctgtcttctt cgttatcctg
    32641 tcacgaagcc gcaatgaaca actcacgcag ctgatgcaac tggtcacgaa tttgtgccgc
    32701 ttcttcgaac tccagattct gcgcgtgttg catcatcaac ccttccagtt catggatttt
    32761 ctgctgcaac gctttaggcg acatatccat cggcacatta tccggctcaa caatcggtcg
    32821 cgattttcct ctgcccttcg ctttggtttt ggcaatgttc tgccccagcg ccaggatatc
    32881 gaccactttc ttgttcaagc cttgcggcgt aatgccgtgt tcctcgttgt acttctgctg
    32941 tttctcgcgg cgacgttcgg tttcgccaat cgctttcgcc attgatgggg tgatcttatc
    33001 gccgtagaga atcgctttac cgttaacgtt acgtgccgca cgaccaatgg tctggatcaa
    33061 cgaacgttcg gaacgcagga agccttcttt gtcagcgtcg aggatcgcca ccagcgaaac
    33121 ttccggcata tccagacctt cgcgcagtaa gttgatccct accaatacgt cgaactcacc
    33181 cagacgcaag tcgcggataa tctccatacg ttcgacggtg tcgatatctg agtgaagata
    33241 acgcacgcgc tcaccgtgtt cttcgagata ttcagtgaga tcttccgcca tccgcttggt
    33301 cagagttgta accagtacgc gttcgttaat tgccgctcgc tgacgaatct ccgaaagaag
    33361 atcatccacc tgtgttgcca ctggccgcac ttcgataatc gggtcgagta agcctgttgg
    33421 acgcaccacc tgatccacca catcgccacc ggatttttcc agctcgtaat tacccggcgt
    33481 cgccgaaaca tagatggttt gcggcgctaa tgcttcgaac tcttcaaatt tcagcggacg
    33541 gttatccagc gctgatggca ggcggaagcc gtactccacc agtgtctctt tacgcgcccg
    33601 gtcaccgcga tacatgccgc caatttgtgg aatggtgacg tgagattcat cgaccaccag
    33661 cagcccatca gcaggcaggt aatcaaacag cgtcggcggt ggctcacccg gtccacgacc
    33721 ggagaggaag cgcgagtagt tctcaatccc cgaacagtag cctagctcgt tcatcatctc
    33781 cagatcaaac tgggtacgct gggtcagccg ctgctcttcc agcagtttgt tgttttccaa
    33841 tagcacttta cgtctggcgg ccagctcttc tttgatctcc tccatcgcct ggacgatgcg
    33901 ctcgcgtggc gtgacgtagt gcgttttcgg gtagatggta aaacgtggaa tagtggaaac
    33961 aatctgcccg gtcagcgggt caaataacga caatcgttcc acttcctcgt caaacagctc
    34021 cacgcgaagt gcaatgtcat ccgattctgc cgggaagata tcaatcacct cgccacgaac
    34081 acggaaagta ccacgctgga aggcttgatc attacgagca tattgcagct ccgccagtcg
    34141 gcgcaggatc gcgcgctgat cgataatcat accgaccgtg agatggagca tcatcttgag
    34201 atataaatca ggatcgccca gaccataaat cgcggaaaca gacgccacca caaccacatc
    34261 acgccgctcc agcatcgctt tggtggcgga caaacgcatc tgttcgatat gttcgtttac
    34321 cgaggcatct ttctcaatga aggtgtcgga actcggtaca taggcctccg gctgatagta
    34381 gtcgtagtag gagacgaaat attccaccgc gttttccggg aagaattctt tcatttcgcc
    34441 atacagctgg gccgccagcg ttttgttggg cgcaagtacc atggttgggc gctgaaggtc
    34501 agcaatgaca ttggcaatgg tgaaggtttt cccggagcca gtcacgccaa gtaacgtctg
    34561 gtgcgccagg ccatcttcca gcccctcttc gagacgtcga atcgcctccg gctgatcgcc
    34621 agaaggttta aaagcggaat tcagtttgaa cggtttactc atgagtcgct acctgaagga
    34681 gttgggcggg caggtatgta attttactcg tcgtacttaa ttatgccaac aaattatact
    34741 ggataaaaaa acagttcatc accataatat ttctgttaca gcgtaaactc cgctgtcaat
    34801 catgagcaaa atttactctg cggcgagata aaactccggc cttaccgggt tatccccaaa
    34861 gcaacggctt ttttaacatt tgtcaagatg attgatgaca gttttatggc aagagatgcc
    34921 tgttcagtga ctgccattgc aattatctaa cctgttaaaa aataaaagat atttctttga
    34981 gccgtcttta acgccaattc gcgtgcaagc cgcgtattct ctcgcttgcc tcgtgttttc
    35041 taactctaat acacatggtt atccacagga atagtggata actgtctgca gcccctatcc
    35101 cccgccgcct gggcaattcc cacattcacc gcatgatgcg gaggtaaaat ttttttcgtg
    35161 atttttttag aatttaattg gttaaattgc agtcaatcgg agacacgatc tcgctcgcaa
    35221 tttaaccaat tacaggatga ctacattaag gcaagattga tgtactttcc ggttgccgca
    35281 ttttctggat tttctgcaag ccagggaatt tcgcccagca gcggcgcggg aatcatgcgg
    35341 gtgagcgtgg tcatatattc agcgtgacgt tttcccggag gcgtgacatc gttcgccacc
    35401 caccccgcca gagtcagtcc ggcgtgtagt attgcctgcg cagtcaacat cgcgtgatta
    35461 atacagccga gtttcacacc aactaccagt atcaccggca gttgttcctg tgttacccaa
    35521 tccgcaaaag tgaaagtgtc agaaagcggc gtaaaccagc cgccagcacc ttccactaac
    35581 acccagtcag cctgttgttc aagcgcgcgt aatccggcgc tcattaccaa tgattctatc
    35641 ggcctgccct cttgcgcgct gatgatatgc ggcgaggtgg gttctgcgaa ggtgtaagga
    35701 tttaccgttg cgtaatccag ctgcaggcta ctgttgcgct gtaacgccag cgcgtcacta
    35761 ttacgtaagc ctttcggggt cttttcgctg ccagaggcca ccggtttata acctgccgtc
    35821 cggtagcctg ctgcctttgc ggcttgtaaa agcgcgcagc tggcgacagt tttccccact
    35881 tcggtatccg ttccggtgac aaaataacat ttactcacga gcaatcactc ccaaaaaaag
    35941 atgatacgtc agaggatatc gcccctgttg ttgcggccag gctagttgca atcgctgcaa
    36001 ctgcgaacgc gttaatattc gcgggtcgcg cccttcatga agatgcgtgg caccgatgcc
    36061 tttcagcgaa cgcatggcac tgagcgcatc atcaaaccac agcgtaatgg gctgaatata
    36121 atgttgataa tgcacggcgt ttagcgactg ttcgatttta tctggcggta aaaagcgatt
    36181 agcatgcggg cgctcgtcca ccgcctgcca cgcctgatgc agttcgggta acgatccctg
    36241 caccagcgtg gtaaacgcga ccacgcctcc ggggcgcacc acccgataca gctcgcggag
    36301 tgccatggat aaattaccgc accactgcac cgcgagattg ctccatgcaa gatcgaacgt
    36361 tgcagtcgct aacggcaggg attcgatatc tcccgccaga taatggtctg cggcatcctt
    36421 ctggcgtgcc tgaacaagca ttggcggaga gagatctaac gcagtcacct gcgcgtgacg
    36481 ctctcgccag cggcggctca tccagcctgg tccacaaccc gcatccagta cgtgggtgta
    36541 tttacgctgt gggagcattg ccagtaaggc gtcagcactc tggcgctgta gatcggcgtg
    36601 ttgctcatag tgtgcggctg cccgtccaaa tgccgctgca atggcttgtt tattaaccgt
    36661 tgccatgcag cacctccagc agacggtcga tatcctgcat ttcatgcgcg gctgttagtg
    36721 ttaagcgcag tcgcgcagta ccagcaggta cggttggcgg gcgaatcgcc gtgacccagc
    36781 agccttgctg gcgcagtttt tctgccagtt gtaacgcacg gctgttatca ccaacaatca
    36841 atggctggat ggcgctgcat gaatcagcaa gcgtaaacgg caaatcctgt actccggcac
    36901 gaaaacgcgt aatgagtgcc gccagttttt cgcgccgtgt atcaccctca tcactgcgaa
    36961 tgaccgccag cgatgcacgt aatgcctgcg cctgagcggg cggcatactg gtgctgtaaa
    37021 taagatggcg ggcaaattgc accagataat ccgccaccgt atcggagcaa agcaccgctg
    37081 ccccgctgac gccaaatcct ttgccaaaag tcaccaccag caattctggt tttacctttt
    37141 gcagccagca gctgccgcgc ccctgctccc cgataacgcc cgtgccgtgg gcgtcatcga
    37201 ccatcaacca gccatcgtgc tgttgcgtta cctgctggat ttcctccagt ggcgcactat
    37261 cgccgtccat gctgaacaca ccttctgtca ctaccagttg ctgccccgga cagggggaag
    37321 caagcagtcg cgccagatga gtgacatcgt tatgagcaaa acggcgaagc ggcgacgggc
    37381 ttaaacttgc agcctccagc aatgaggcat ggctaagccg gtcggcaaca atacggtcct
    37441 ctttcgccat catcgcggca atgactgcct gattagccgc aaaaccggag ataaacagca
    37501 gcgcccgcga atagcccagc cactcggcca gttcttcttc cagcgcctga tgtgccacgc
    37561 tataaccgct gacgtgaccg gagccgccgc taccgacgcc aaattgctcc gcactctgct
    37621 tccaggcacg gataatttgc ggatgatggc ttaaacctaa ataatcgtta ctggaaaagt
    37681 tcagatactg gcgatcgtcc gccaccagcc agcgtccggc cccttgcgcc accggataac
    37741 ggcgacgcag ggcatcggca gcacgccgcg catcgagcgc cgcgttgatt ttctcctgcc
    37801 agctcatagt gctgccgcgt tgtaatattc gtcagtgtcc ggggtcatca gtgcctgctc
    37861 cagacgctgc tgttgttcgt tatcccctgc cagcacggca gtttgctgtg gatttagccc
    37921 cagtttgcgg aacagttgca ggtctttatc ttcttccgga ttcggcgtgg ttagcagttt
    37981 gcaaccgtag aaaatcgagt tagccccggc cataaagcac atcgcctgag tctgttcatt
    38041 catctgctcg cgtccggcag aaaggcgcac gtatgaggtc ggcatcatga tccgcgctac
    38101 ggcaatggtg cgaataaaat caaaggcatc gacatcatcg ttatctgcca gtggagtacc
    38161 tttcaccttc acgagcatgt tgattggcac gctttccggc ggcgtcggca ggtttgccag
    38221 ttgcagtaat aatccggcac gatcttttac cgtttcgcct aaacccacaa tgccacctga
    38281 gcagactttg atcccggcat cgcgcacttt ttccagcgta tcaaggcgtt cctgataagt
    38341 acgtgtggtg atgatattgc cgtaaaactc cggcgaggtg tcgaggttat ggttgtagta
    38401 atccagcccg gcattcgcaa ggcgctgcgc ctgagattca ctcaacgtac ccagcgtcat
    38461 acacgcctcc agccccatcg cttttactcc ctgcaccatt tgttccaggt agggcatatc
    38521 gcgttcgtgg ggattcttcc acgccgcgcc catgcagaag cgcgtcgatc ctgccgcttt
    38581 cgctttgcgc gccgacgcca gcacctgttc aacttccatc aaccgctcgg cttccagccc
    38641 ggttttgtag cgcgagcttt gcgggcagta tttgcaatct tccggacaag ctccggtctt
    38701 aattgacagc aaagtgctga cctgcacctg acgtggatcg aaatgctgac gatgcacctg
    38761 ctgcgcttca aacagcagat ccagcaacgg tttttcaaat aattctgtga cttgcgacaa
    38821 tgtccagcgt gggcggtgag ccatggggct tctccaaaac gtgttttttg ttgttaattc
    38881 ggtgtagact cgtaaaccta aatcttttca atttggttta caagtcgatt atgacaacgg
    38941 acgatcttgc ctttgaccaa cgccatatct ggcacccata cacatccatg acctccccgc
    39001 taccggttta tccggtggcg agcgccgaag gttgcgagct aattttgtct gacggcagac
    39061 gcctggttga cggtatgtcg tcctggtggg cggcgatcca cggctacaat cacccgcagc
    39121 ttaatgcggc gatgaagacg caaattgatg ccatgtcgca tgtgatgttt ggtggtatca
    39181 ctcatgcgcc agccattgag ctgtgccgca aactggtggc gatgacgccg caaccgctgg
    39241 aatgcgtttt tctcgcggac tccggttccg tagcggtgga agtggcgatg aaaatggcgt
    39301 tgcagtactg gcaggccaaa ggcgaagcgc gccagcgttt tctgaccttc cgcaatggtt
    39361 atcatggcga tacctttggc gcgatgtctg tgtgcgatcc ggacaactca atgcacagcc
    39421 tgtggaaagg ctatctgcca gaaaacttgt ttgctcccgc cccgcaaagc cgcatggatg
    39481 gtgaatggga tgaacgcgat atggtgggct ttgcccgcct gatggcggca catcgtcatg
    39541 aaatcgccgc cgtgatcatt gaaccgattg tccagggcgc aggcgggatg cgcatctacc
    39601 atccggaatg gttaaaacga atccgcaaaa tgtgcgatcg cgaaggtatc ttgctgattg
    39661 ctgacgagat cgccaccgga tttggtcgta ccggcaaact gtttgcctgt gaacatgcag
    39721 atatcgcgcc ggatattttg tgtctcggta aagccttaac cggcggcaca atgacccttt
    39781 ccgccacgct caccacgcgc gaggttgcgg aaaccatcag taacggcgaa gccggctgct
    39841 ttatgcatgg gccaactttt atgggcaatc cgctggcctg cgcagcagca aacgccagcc
    39901 tggcgattct cgaatctggc gactggcagc atcaggtggc ggctattgaa gggcagctac
    39961 gcgagcaact tgcccccgcc tgtgacgccg aaatggttgc cgatgtgcgc gtactggggg
    40021 caatcggcgt ggtcgaaacc actcgtccgg tgaatatggc agcgctgcaa aaattctttg
    40081 tcgaacaggg tgtctggatc cggccttttg gcaaactgat ttacctgatg ccgccctata
    40141 ttattctccc gcaacagttg cagcgtctga ccgtagcggt taaccgtgcg gtgcaggatg
    40201 aaacattttt ttgccaataa ccgggagtca gcgtgagggg ttctggctac actttctgca
    40261 aactagaaag gagggttcat gaaactcatc agtaatgatc tgcgcgatgg cgataagttg
    40321 ccacatcgtc atgtctttaa cggcatgggt tacgatggcg ataatatttc accgcatctg
    40381 gcgtgggatg atgttcctgc gggaacgaaa agttttgttg tcacctgcta cgacccggat
    40441 gcgccaaccg gctccggctg gtggcactgg gtagttgtta acttacccgc tgatacccgc
    40501 gtattaccgc aagggtttgg ctctggtctg gtagcaatgc cagacggcgt tttgcagacg
    40561 cgtaccgact ttggtaaaac cgggtacgat ggtgcagcgc cgccgaaagg cgaaacccat
    40621 cgctacattt ttaccgttca tgcgctggat gtagaacgta ttgatgtcga tgaaggtgcc
    40681 agcggcgcga tggtcgggtt taacatccat ttccactctc tggcaagcgc atcgattacc
    40741 gcgatgttca gttaattact ctgccagatg gcgaaaagcc atctggtatc acttaaaggt
    40801 attaaaaaca actttttgtc tttttacctt cccgtttcgc taaagttagt ataaaaaagc
    40861 aggcctcaac ggattcattt ttctatttca tagcccggag caacctgtga acacattttc
    40921 agtttcccgt ctggcgctgg cattggcttt tggcgtgacg ctgaccgcct gtagctcaac
    40981 accgcccgat caacgtcctt ctgatcaaac cgcgcctggt acctcttctc gcccgattct
    41041 gtcggcaaaa gaagcgcaga atttcgatgc tcaacactat tttgcatccc tgacaccagg
    41101 tgcggcagcg tggaatcctt ccccgattac cctgcctgca caacctgact ttgttgtcgg
    41161 cccggcgggt actcaaggtg taacgcatac cacgattcag gcggcggtag atgcggcaat
    41221 tatcaagcgt accaacaagc gccagtatat tgccgtgatg cctggtgagt atcagggaac
    41281 ggtatatgtc cctgccgctc cgggtggaat tactctgtac ggtacaggtg aaaaaccgat
    41341 tgatgtgaag attgggcttt cccttgatgg gggcatgagc cccgccgact ggcgtcacga
    41401 cgtcaacccg cgcggcaaat atatgccagg taaaccagcg tggtatatgt acgatagctg
    41461 ccagagcaaa cgcagcgaca gtatcggcgt tctctgctct gcggtcttct ggtcacaaaa
    41521 caatggactg caactgcaaa acctgaccat cgaaaacacg ctgggcgata gcgtagatgc
    41581 gggtaaccat ccggcggtgg cactgcgtac tgatggtgac caggtacaga ttaacaacgt
    41641 taacattctc ggtcgtcaga acaccttctt tgtcaccaac agcggtgtgc agaaccgtct
    41701 ggaaacgaat cgtcagccgc gtacgctggt gaccaacagc tacattgaag gggatgtgga
    41761 tatcgtttct ggtcgcggcg cagtggtgtt cgataatacc gaattccgcg tggtgaactc
    41821 acgtactcag caagaagcgt atgtgtttgc accggctacg ctgtccaaca tttactacgg
    41881 tttcctcgcc gtaaacagtc gtttcaatgc ttccggtgat ggcgtggcgc aactgggccg
    41941 ctcgctggat gttgatgcca ataccaacgg tcaggtggtg atccgtgata gcgccatcaa
    42001 cgaaggtttt aacacagcca aaccgtgggc tgatgcggtg atttccaatc gtccattcgc
    42061 gggtaacacc ggcagcgttg atgataacga cgaagtacag cgcaatctga atgacactaa
    42121 ctacaaccgc atgtgggaat acaataaccg cggcgtgggt agcaaagtgg ttgcagaggc
    42181 gaagaagtag gagctgttaa ctatgtgccg gatgcggcgt aaacgcctta tccggcctac
    42241 ggttcgatgc gatttgtagg tcggataaga tgcgccagca tcgcatccga caataagtgc
    42301 cggatgcgac ctacattcac atggcgcttt ttacatctga cggtttttat tgaagttaat
    42361 caaactaccc gccttgataa tctcgcgctc ttcagcagtc agactttcca tatagagcgt
    42421 aatctccgtt accggtgcat cttcatggat cacataacct ttaaacgtcg ttccaggatt
    42481 atccagcgcc gctttaatgc cagggatgta aatgtaatcc cccacttcaa acgttggtgc
    42541 ttccgccatt tgcagcggta acatccccca gttgatgacg ttagaacggt agcgtttagt
    42601 cgcgtattcc tcggcaatat tcgccagacc gccaatcaca cgctggcagc tcgccgcctg
    42661 ctcacgcgca gaaccatcgc ctggtttcac cgcatagacc atgctgccaa tttcagtttg
    42721 cagcggatca atatgctcct gaccagcaat ctgcttaatg cgcgcaaaca cctctgtcag
    42781 ctcgctgaca ttccccgcca gacgctgatt ttccagctca gcagtcgctt tacttctgcc
    42841 aacataaccg ggatcgcggc gtgacagggt aaactccgcc agaccaatcg gatttgaacg
    42901 ataagaagag gtttcaccgg aaggaatcag ttcgtcggtg gtggtgactt cgtcgaggat
    42961 cttcgagcac actttgagga cgatattgtc agtcagcgcg cccaattccg gccagtcttt
    43021 gatgttcggt ccgtagatca gcggttgctg ggtcgcccct ttcacaaagc cttgatagac
    43081 gcgattttta tacggcgtta catcgaaggc gtactccggc acgttgtccc agcaatcgag
    43141 ttcgctggca gaggttaaat agccaccgtt tgccgcagtt gcagcgatag aacgagcgtc
    43201 catcaacgcc accgctgaca tctgcccatt agctggctta gagccttcgc ggttcgggaa
    43261 gttacgcgtg gtgtggcgaa tactcaaacc gttgttgatt ggcgtatcgc ccgcaccaaa
    43321 gcatgggccg cagaacgcgg ttctgatgat tgcgcctgcg ccaatcaaat ctgctaccac
    43381 accttttttg gcgagatcca taaacaccgg ctgtgatgac gggtaaaccg ccaatgagaa
    43441 ggtatcgttg ccgcaggatt gaccgcgcag cgcattcgcc gccgcgatga cgttttcgta
    43501 gttaccgcca gaacagcccg cgataatccc ctgctgcact ttcaggcgac cattttccac
    43561 tttatccagc agcgagagtt tggctttacc gtgcgccacg cgttcggact caatttcaat
    43621 ctcacgcaga atgtcggtca ggttctggtt cagcgtgtcg atttcataca cgttgctcgg
    43681 gtggaatggc agcgcaatca ttggtttgat ggcgcttaaa tcaacgctga tgcagccatc
    43741 gtagtacgcc attggttgag ggttaagttg gcagtaatcc tggccgcgac cgtgcagcgc
    43801 cagccagtta tggacttctt catcggtttg ccagacagaa cttaaacagg tcgtttcagt
    43861 ggtcatcacg tcaacgctgt tacggaaatc ggtagagagc gctgcaacac cgggacctac
    43921 gaactccatc actttgtttt tgacgtaacc gtttttgaac acggcaccga tgatagccag
    43981 cgccacatcc tgcggcccca catacggcgc aggttttccg gtcagatgca ccgcaaccac
    44041 gcccggatag tcgatatccc aggtgtcatt aagcagctgt tttaccaact cgccgccgcc
    44101 ctcgccaacc gccattgtcc ctaacgcacc gtaacgggta tggctgtctg acccgaggat
    44161 cattttgccg ccgcccgcca tcatttcacg catatattga tggatgaccg caatatgcgg
    44221 tggcacaaaa ataccgccat aacgctgggc cgccgataaa ccaaaaacat ggtcatcacc
    44281 gttaatggtg ccgcccacag cacagagtga gttatggcag ttggtcagca catacggcag
    44341 cgggaaacgt tccataccgg acgctttagc ggtctgtaca ataccaacaa aggtaatatc
    44401 gtgagaggct aatgaatcaa acttaatttt aagtttatcc atatttccgg acgtattatg
    44461 agaagagaga atagaccagg caatagtgcc tttttttgct tcttcttttt ttatttcgcc
    44521 ggtgaaatgt tcttcggcaa ttatttcgtt attgctggcg agaaacacgc ctttttcaga
    44581 taacttgatc atcaaatgct ccagatatct gcgcaccgtg aagtgcgcag aaagataata
    44641 aatggatata ctctaaataa ttcgagttgc aggaaggcga caagcgagtg aatccccagg
    44701 agcttacata agtaagtgac tggggtgagc gaacgcagac gcagcacatg caacttgaag
    44761 tatgacgagt atatttacag catgttccag ccgatcagca tattccacca ccacacgccg
    44821 agggtgatat gcaccaggaa ggttaatatc gtcagtaccg caccgaccaa ccaccaggat
    44881 ttaatatcgt tgtaacccac gccaaagatg accggacctg ccgcgccgcc atagtgagta
    44941 accatgccgc cataggagtt agagaacaac agtgccagcg cggttaacat taacggtgcg
    45001 ccggagacgt tcgccagcat ggcaaataca ggtaacatag caacgatata ggcactaccg
    45061 gaagcgaaga aataacgcac gatgatgctg aggaaaataa taacgaagaa agcaacgtta
    45121 ccgtgaccat caaatgccag gttattttta aagacttcag ctaaccattc aaagaattta
    45181 actttcgata ataaggagct taagccgata ataccgccgt accagattaa ggtattccag
    45241 ccgcctttat ttttaaccac gtcttcccag gtaacgatac ccagcagcag catagtcgcc
    45301 ataacaacga ttgccacggt ggattcatca acccccagtg acttactgaa aatccagccc
    45361 agcagcgcca gcacgaagac accgagcagc attttttcgc ggattttcat cggtcccagt
    45421 tcggcaaggc ccgctttggc gatggttttg ttatccacct tcttaatttc tggcggatac
    45481 atggtgtaaa tcaccagcgg ggtgaccagc agcataatga tacccggcaa tccagcggcc
    45541 agcgcccatc caccccagct aatttgcagg tgcagaatgt cgttgatcat tttcagcgcc
    45601 agaatgttcc ccgccattgc ggtaaagaac atatagctgg tggttttagt gaccatgtaa
    45661 atggacatca tcaggtaatg tccaacacga cgcggacttt tttccggttc tgatcccaaa
    45721 gccactgcca cgctgttgat gatcggtaac acgatgccgc ccgcacgcgc ggtgttagac
    45781 ggtgttgccg gagccagtac cagatcgaga aataccgtaa cgtaacccag acccagggta
    45841 gtgctaccaa ttttaccaat cagcagatag gcaatacgtt tacctaagcc tgtggttaca
    45901 aacgcggcgc ttaaagtaaa cgcagaaaac accagccagg tggtaccgga agagtaaccg
    45961 cttaatacgg cggtggtttt aaattcccca cctgataagt tacccaccac taccatcgat
    46021 gcggcgacgg caattaacag tacgacaggt tccgggaaag gcttgataac cagccccaca
    46081 atggccgcca ggtaaatacc aaaaagcacc cacgccagtt cgcttaatcc tgccggagct
    46141 ggcataaagc caataataca tgggatcgct aatatcagaa ttagcttcca taacgatttt
    46201 ttattcattt aatatcctgt tctttctgta tgactcatac agaattcctt tattgattgt
    46261 cattatcacg acaacaaaat ttttcaggga agataaactt caccggaaaa tatttttctt
    46321 gtcgtccgaa taacagatgc gcgtaacgtc gtggcatcct gaccttcatt acttaaatga
    46381 acgtcgagcg caccactggg gtgttcaata ttgatattgc cgtatcctac agaagggacg
    46441 atttgtcggg tgacggtgcc ttccaatgca caactacagg aaatagcaat agcaccggtt
    46501 atcgccaaag cgcgatggca agaatgcggc ataaaataac gcacattaat tgccccgcct
    46561 ttctgcgctg gagaaataag tacaggttta gggataacca tattactgac atcgcctaag
    46621 cccattgctt tacccgcttg tagacggata gattcaatgc tggctaataa tgctttgtcg
    46681 gcatccagct ctgccggtaa ttcatagcct gtttttccca gatattcagc aggaataatg
    46741 acaactggca tcgccatatc gatacaggtc accgggacat cgtcaaaata atcaatctga
    46801 ttatccgtcg ggaagacttt tccggttttc gttccggcgg cattcaggaa agtgagcgca
    46861 accggtgcgg cagtacccgg tacgccgtca attctggcgc taccctcgta ctcgacaaca
    46921 ccatttggcg tttgcacatc agcttcgata aacgtacccg tattgacgtt gcggatacgt
    46981 acgcgggtaa ctggcgaagt cgctgcaatc aaaccatttt caatggcgaa tgccccaacg
    47041 ccagacagca tattgccgca gttaggcgtg gtatcgacac gttgctcatg gacgataact
    47101 tgagcaaaca gataatcgac atctgcacgc ggatcgctgg agcggctaat aatggcgact
    47161 ttactggtca gcggattacc gccgccaata ccgtcaattt ccagatcgtt accggaaccc
    47221 ataattgcca tcaatatttt atcgcgctgc gtttgatctt cgggtagatg ttccgctaac
    47281 aggaacgcgc ccctcgaggt tccacctcgc atcatcacgc agggtatttt tttcataata
    47341 atgccctcaa tttaaaaaca taacttattg cg
//
LOCUS       DAZFLD010000010        45688 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.010, whole genome shotgun sequence.
ACCESSION   DAZFLD010000010 DAZFLD010000000
VERSION     DAZFLD010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 45688)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45688)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45688
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.010"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(356..442)
                     /locus_tag="ACHKBL_000511"
     tRNA            complement(356..442)
                     /locus_tag="ACHKBL_000511"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(406..408),aa:Leu,seq:gag)
     gene            complement(457..789)
                     /gene="secG"
                     /locus_tag="ACHKBL_000512"
     CDS             complement(457..789)
                     /gene="secG"
                     /locus_tag="ACHKBL_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HFO5817434.1"
                     /translation="MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFG
                     SSGSGNFMTRMTALLATLFFIISLVLGNINSNKTNKGSEWENLSAPAKTEQIQPAAPA
                     KPTSDIPN"
     gene            complement(1017..2354)
                     /gene="glmM"
                     /locus_tag="ACHKBL_000513"
     CDS             complement(1017..2354)
                     /gene="glmM"
                     /locus_tag="ACHKBL_000513"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HFO5817435.1"
                     /translation="MSNRKYFGTDGIRGRVGDAPITPDFVLKLGWAAGKVLARHGSRK
                     IIIGKDTRISGYMLESALEAGLAAAGLSALFTGPMPTPAVAYLTRTFRAEAGIVISAS
                     HNPFYDNGIKFFSIDGTKLPDAVEEAIEAEMEKEISCVDSAELGKASRIVDAAGRYIE
                     FCKATFPNELSLSELKIVVDCANGATYHIAPNVLRELGANVIAIGCEPNGVNINAEVG
                     ATDVRALQARVLAEKADLGIAFDGDGDRVIMVDHEGNKVDGDQIMYIIAREGLRQGQL
                     RGGAVGTLMSNMGLELALKQLGIPFARAKVGDRYVLEKMQEKGWRIGAENSGHVILLD
                     KTTTGDGIVAGLQVLAAMARNHMSLHDLCSGMKMFPQILVNVRYTAGSGDPLEHESVK
                     AVTAEVEAALGSRGRVLLRKSGTEPLIRVMVEGEDEAQVTEFAHRIADAVKAV"
     gene            complement(2347..3195)
                     /gene="folP"
                     /locus_tag="ACHKBL_000514"
     CDS             complement(2347..3195)
                     /gene="folP"
                     /locus_tag="ACHKBL_000514"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HFO5817436.1"
                     /translation="MKLFAQGTSLDLSHPHVMGILNVTPDSFSDGGTHNSLIDAVKHA
                     NLMINAGATIIDVGGESTRPGAAEVSVEEELQRVIPVVEAIAQRFEVWISVDTSKPEV
                     IRESAKVGAHIINDIRSLSEPGALEAAAETGLPVCLMHMQGNPKTMQEAPKYHDVFAE
                     VNRYFIEQIARCEQAGIAKEKLLLDPGFGFGKNLSHNYSLLARLAEFHHFNLPLLVGM
                     SRKSMIGQLLNVGPSERLSGSLACAVIAAMQGAHIIRVHDVKETVEAMRVVEATLSAK
                     ENKRYE"
     gene            complement(3285..5219)
                     /gene="ftsH"
                     /locus_tag="ACHKBL_000515"
     CDS             complement(3285..5219)
                     /gene="ftsH"
                     /locus_tag="ACHKBL_000515"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HFO5817437.1"
                     /translation="MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNND
                     QVREARINGREINVTKKDSNRYTTYIPVQDPKLLDNLLTKNVKVVGEPPEEPSLLASI
                     FISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVAGCDEA
                     KEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISG
                     SDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLN
                     QMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVH
                     MRRVPLAPDIDAAIIARGTPGFSGADLANLVNEAALFAARGNKRVVSMVEFEKAKDKI
                     MMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLP
                     EGDAISASRQKLESQISTLYGGRLAEEIIYGPEHVSTGASNDIKVATNLARNMVTQWG
                     FSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQLLTD
                     NMDILHAMKDALMKYETIDAPQIDDLMARRDVRPPAGWEEPGASNNSGDNGSPKAPRP
                     VDEPRTPNPGNTMSEQLGDK"
     gene            complement(5319..5948)
                     /gene="rlmE"
                     /locus_tag="ACHKBL_000516"
     CDS             complement(5319..5948)
                     /gene="rlmE"
                     /locus_tag="ACHKBL_000516"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016436 - rRNA (uridine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HFO5817438.1"
                     /translation="MTGKKRSASSSRWLQEHFSDKYVQQAQKKGLRSRAWFKLDEIQQ
                     SDKLFKPGMTVVDLGAAPGGWSQYVVTQIGGKGRIIACDLLPMDPIVGVDFLQGDFRD
                     ELVMKALLERVGDSKVQVVMSDMAPNMSGTPAVDIPRAMYLVELALEMCRDVLAPGGS
                     FVVKVFQGEGFDEYLREIRSLFTKVKVRKPDSSRARSREVYIVATGRKP"
     gene            6074..6367
                     /gene="yhbY"
                     /locus_tag="ACHKBL_000517"
     CDS             6074..6367
                     /gene="yhbY"
                     /locus_tag="ACHKBL_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HFO5817439.1"
                     /translation="MNLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHH
                     ELIKVKIATEDRETKTLIVEAIVRETGACNVQVIGKTLVLYRPTKERKISLPR"
     gene            complement(6523..6999)
                     /gene="greA"
                     /locus_tag="ACHKBL_000518"
     CDS             complement(6523..6999)
                     /gene="greA"
                     /locus_tag="ACHKBL_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HFO5817440.1"
                     /translation="MQAIPMTLRGAEKLREELDFLKSVRRPEIIAAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIKDIEAKLSNAQVIDVTKMPNNGRVIFGATVTVLNLDSDEE
                     QTYRIVGDDEADFKQNLISVNSPIARGLIGKEEDDVVVIKTPGGEVEFEVIKVEYL"
     gene            7247..8680
                     /gene="dacB"
                     /locus_tag="ACHKBL_000519"
     CDS             7247..8680
                     /gene="dacB"
                     /locus_tag="ACHKBL_000519"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004185 - serine-type carboxypeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HFO5817441.1"
                     /translation="MRFSRFIIGLTSCIAFSVQAANVDEYITQLPAGANLALMVQKVG
                     ASAPAIDYHSQQMALPASTQKVITALAALIQLGPDFRFTTTLETKGNVENGVLKGDLV
                     ARFGADPTLRRQDIRNMVATLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCF
                     SAPPAAAIVDRNCFSVSLYSAPKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCE
                     LDVVPGDLNRFTLTGCLPQRSEPLPLAFAVQDGASYAGAILKDELKQAGITWSGTLLR
                     QTQVNEPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGHARFNVPGTWRAGSD
                     AVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPL
                     AGYDGSLQYRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAV
                     EPADQRNRRIPLVRFESRLYKDIYQNN"
     gene            complement(8721..9893)
                     /gene="cgtA"
                     /locus_tag="ACHKBL_000520"
     CDS             complement(8721..9893)
                     /gene="cgtA"
                     /locus_tag="ACHKBL_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HFO5817442.1"
                     /translation="MKFVDEASILVVAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGD
                     VWMEADENLNTLIDYRFEKSFRAERGQNGASRDCTGKRGKDVTIKVPVGTRVIDQGTG
                     ETMGDMTKHGQRLLVAKGGWHGLGNTRFKSSVNRTPRQKTNGTPGDKRELLLELMLLA
                     DVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRMDNEKSFVVADIPGLI
                     EGAAEGAGLGIRFLKHLERCRVLLHLIDIDPIDGTDPVENARIIISELEKYSQDLAAK
                     PRWLVFNKIDLLDKAEAEEKAKAIAEALGWEDKYYLISAASGLGVKDLCWDVMTFIIE
                     NPVVQAEEAKQPEKVEFMWDDYHRQQLEEIAEEDDEDWDDDWDEDDEEGVEFIYKR"
     gene            complement(9909..10874)
                     /gene="yhbE"
                     /locus_tag="ACHKBL_000521"
     CDS             complement(9909..10874)
                     /gene="yhbE"
                     /locus_tag="ACHKBL_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFO5817443.1"
                     /translation="MKQQAGIGILLALTTAICWGALPIAMKQVLEVMEPPTIVFYRFL
                     MASIGLGAILAVKKRLPPLRVFRKPRWLILLAVATAGLFGNFILFSSSLQYLGPTASQ
                     VIGQLSPVGMMVASVFILKEKMRSTQVVGALMLLSGLVMFFNTSLVEIFTKLTDYTWG
                     VIFGVGAATVWVSYGVAQKVLLRRLASPQILFLLYTLCTIALFPLAKPGVIAQLSHWQ
                     LACLIFCGLNTLVGYGALAEAMARWQAAQVSAIITLTPLFTLFFSDLLSLAWPDFFAR
                     PMLNLLGYLGAFVVVAGAMYSAIGHRIWGGLRKHTTVVSQPRAGE"
     gene            complement(11001..11258)
                     /gene="rpmA"
                     /locus_tag="ACHKBL_000522"
     CDS             complement(11001..11258)
                     /gene="rpmA"
                     /locus_tag="ACHKBL_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HFO5817444.1"
                     /translation="MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE"
     gene            complement(11279..11590)
                     /gene="rplU"
                     /locus_tag="ACHKBL_000523"
     CDS             complement(11279..11590)
                     /gene="rplU"
                     /locus_tag="ACHKBL_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HFO5817445.1"
                     /translation="MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANG
                     EEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGIS
                     A"
     gene            11849..12820
                     /gene="ispB"
                     /locus_tag="ACHKBL_000524"
     CDS             11849..12820
                     /gene="ispB"
                     /locus_tag="ACHKBL_000524"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HFO5817446.1"
                     /translation="MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGG
                     KRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAA
                     FGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
                     ENYMRVIYSKTARLFEAAAQCSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNA
                     DGEQLGKNVGDDLNEGKPTLPLLHAMHHGTPEQAQMIRTAIEQGNGRHLLEPVLEAMN
                     ACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALIGLAHIAVQRDR"
     gene            13048..13326
                     /gene="sfsB"
                     /locus_tag="ACHKBL_000525"
     CDS             13048..13326
                     /gene="sfsB"
                     /locus_tag="ACHKBL_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SfsB"
                     /protein_id="HFO5817447.1"
                     /translation="MESNFIDWHPADIIAGLRKKGTSMAAESRRNGLSSSTLANALSR
                     PWPKGEMIIAKALGTDPWIIWPSRYHDPQTHEFIDRTQLMRSYTKPKK"
     gene            complement(13374..14633)
                     /gene="murA"
                     /locus_tag="ACHKBL_000526"
     CDS             complement(13374..14633)
                     /gene="murA"
                     /locus_tag="ACHKBL_000526"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFO5817448.1"
                     /translation="MDKFRVQGPTKLQGEVTISGAKNAALPILFAALLAEEPVEIQNV
                     PKLKDVDTSMKLLSQLGAKVERNGSVHIDARDVNVFCAPYDLVKTMRASIWALGPLVA
                     RFGQGQVSLPGGCTIGARPVDLHISGLEQLGATIKLEEGYVKASVDGRLKGAHIVMDK
                     VSVGATVTIMCAATLAEGTTIIENAAREPEIVDTANFLITLGAKISGQGTDRIVIEGV
                     ERLGGGVYRVLPDRIETGTFLVAAAISRGKIICRNAQPDTLDAVLAKLRDAGADIEVG
                     EDWISLDMHGKRPKAVNVRTAPHPAFPTDMQAQFTLLNLVAEGTGFITETVFENRFMH
                     VPELSRMGAHAEIESNTVICHGVEKLSGAQVMATDLRASASLVLAGCIAEGTTVVDRI
                     YHIDRGYERIEDKLRALGANIERVKGE"
     gene            complement(14688..14942)
                     /gene="ibaG"
                     /locus_tag="ACHKBL_000527"
     CDS             complement(14688..14942)
                     /gene="ibaG"
                     /locus_tag="ACHKBL_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family iron metabolism protein IbaG"
                     /protein_id="HFO5817449.1"
                     /translation="MENNEIQSVLMNALSLQEVHVSGDGSHFQVIAVGELFDGMSRVK
                     KQQTVYGPLMEYIADNRIHAVSIKAYTPAEWARDRKLNGF"
     gene            complement(15102..15395)
                     /gene="mlaB"
                     /locus_tag="ACHKBL_000528"
     CDS             complement(15102..15395)
                     /gene="mlaB"
                     /locus_tag="ACHKBL_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HFO5817450.1"
                     /translation="MSESLSWMQTGDTLALSGELDQDVLLPLWEMREEAVKGITCIDL
                     SRVSRVDTGGLALLLHLIDLAKKQGNNVTLQGVNDKVYTLAKLYNLPADVLPR"
     gene            complement(15395..16030)
                     /gene="mlaC"
                     /locus_tag="ACHKBL_000529"
     CDS             complement(15395..16030)
                     /gene="mlaC"
                     /locus_tag="ACHKBL_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HFO5817451.1"
                     /translation="MFKRLMMVALLVIAPLSAATAADQTNPYKLMDEAAQKTFDRLKN
                     EQPQIRANPDYLRTIVDQELLPYVQVKYAGALVLGQYYKSATPAQRDAYFAAFREYLK
                     QAYGQALAMYHGQTYQIAPEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGN
                     WQAYDMIAEGVSMITTKQNEWGTLLRTKGIDGLTAQLKSISQQKITLEEKK"
     gene            complement(16049..16600)
                     /gene="mlaD"
                     /locus_tag="ACHKBL_000530"
     CDS             complement(16049..16600)
                     /gene="mlaD"
                     /locus_tag="ACHKBL_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HFO5817452.1"
                     /translation="MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTEPTYTLYA
                     TFDNIGGLKARSPVSIGGVVVGRVADITLDPKTYLPRVTLEIEQRYNHIPDTSSLSIR
                     TSGLLGEQYLALNVGFEDPELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNK
                     NSGDAPAAAPGNNETTEPVGTTK"
     gene            complement(16605..17387)
                     /gene="mlaE"
                     /locus_tag="ACHKBL_000531"
     CDS             complement(16605..17387)
                     /gene="mlaE"
                     /locus_tag="ACHKBL_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HFO5817453.1"
                     /translation="MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHAPLL
                     VRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPV
                     VAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTV
                     IFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLVNCLIKSVVFAITVTWISL
                     FNGYDAIPTSAGISRATTRTVVHSSLAVLGLDFVLTALMFGN"
     gene            complement(17395..18204)
                     /gene="mlaF"
                     /locus_tag="ACHKBL_000532"
     CDS             complement(17395..18204)
                     /gene="mlaF"
                     /locus_tag="ACHKBL_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HFO5817454.1"
                     /translation="MEQSVANLVDMRDVSFTRGNRCIFDNISLTVPRGKITAIMGPSG
                     IGKTTLLRLIGGQIAPDHGEILFDGENIPAMSRSRLYTVRKRMSMLFQSGALFTDMNV
                     FDNVAYPLREHTQLPAPLLHSTVMMKLEAVGLRGAAKLMPSELSGGMARRAALARAIA
                     LEPDLIMFDEPFVGQDPITMGVLVKLISELNSALGVTCVVVSHDVPEVLSIADHAWIL
                     ADKKIVAHGSAQALQANPDPRVRQFLDGIADGPVPFRYPAGDYHADLLPGS"
     gene            18414..19391
                     /gene="yrbG"
                     /locus_tag="ACHKBL_000533"
     CDS             18414..19391
                     /gene="yrbG"
                     /locus_tag="ACHKBL_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HFO5817455.1"
                     /translation="MLLATALLIVGLLLVVYSADRLVFAASILCRTFGIPPLIIGMTV
                     VSIGTSLPEIIVSLAASLHEQRDLAVGTALGSNIINILLILGLAALVRPFTVHSDVLR
                     RELPLMLLVSVVAGSVLYDGQLSRSDGIFLLFLAVLWLLFIVKLARQAERQGTDSLTR
                     EQLAELPRDGGLPVAFLWLGIALIIMPVATRMVVDNATVLANYFAISELTMGLTAIAI
                     GTSLPELATAIAGVRKGENDIAVGNIIGANIFNIVIVLGLPALITPGEIDPLAYSRDY
                     SVMLLVSIIFALLCWRRSPQPGRGVGVLLTGGFIVWLAMLYWLSPILVE"
     gene            19405..20391
                     /gene="kdsD"
                     /locus_tag="ACHKBL_000534"
     CDS             19405..20391
                     /gene="kdsD"
                     /locus_tag="ACHKBL_000534"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase KdsD"
                     /protein_id="HFO5817456.1"
                     /translation="MSHVELQPGFDFQQAGKEVLAIERECLAELDQYINQNFTLACEK
                     MFWCKGKVVVMGMGKSGHIGRKMAATFASTGTPSFFVHPGEAAHGDLGMVTPQDVVIA
                     ISNSGESSEITALIPVLKRLHVPLICITGRPESSMARAADVHLCVKVAKEACPLGLAP
                     TSSTTATLVMGDALAVALLKARGFTAEDFALSHPGGALGRKLLLRVNDIMHTGDEIPH
                     VKKTASLRDALLEVTRKNLGVTVICDDNMMIEGIFTDGDLRRVFDMGVDVRQLSIADV
                     MTPGGIRVRPGILAVEALNLMQSRHITSVMVADGDHLLGVLHMHDLLRAGVV"
     gene            20412..20978
                     /gene="kdsC"
                     /locus_tag="ACHKBL_000535"
     CDS             20412..20978
                     /gene="kdsC"
                     /locus_tag="ACHKBL_000535"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HFO5817457.1"
                     /translation="MSKAGASLATCYGPVSADVIAKAENIRLLILDVDGVLSDGLIYM
                     GNNGEELKAFNVRDGYGIRCALTSDIEVAIITGRKAKLVEDRCATLGITHLYQGQSNK
                     LIAFSDLLEKLAIAPENVAYVGDDLIDWPVMEKVGLSIAVADAHPLLIPRADYVTRIA
                     GGRGAVREVCDLLLLAQGKLDEAKGQSI"
     gene            20975..21550
                     /gene="lptC"
                     /locus_tag="ACHKBL_000536"
     CDS             20975..21550
                     /gene="lptC"
                     /locus_tag="ACHKBL_000536"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HFO5817458.1"
                     /translation="MSKARRWVIIVLSLAVLVMIGINMAEKDDTAQVVVNNNDPTYKS
                     EHTDTLVYNPEGALSYRLIAQHVEYYSDQAVSWFTQPVLTTFDKDKIPTWSVKADKAK
                     LTNDRMLYLYGHVEVNALVPDSQLRRITTDNAQINLVTQDVTSEDLVTLYGTTFNSSG
                     LKMRGNLRSKNAELIEKVRTSYEIQNKQTQP"
     gene            21519..22076
                     /gene="lptA"
                     /locus_tag="ACHKBL_000537"
     CDS             21519..22076
                     /gene="lptA"
                     /locus_tag="ACHKBL_000537"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter
                     substrate-binding protein LptA"
                     /protein_id="HFO5817459.1"
                     /translation="MKFKTNKLSLNLVLASSLLAASIPAFAVTGDTDQPIHIESDQQS
                     LDMQGNVVTFTGNVIVTQGTIKINADKVVVTRPGGEQGKEVIDGYGKPATFYQMQDNG
                     KPVEGHASQMHYELAKDFVVLTGNAYLQQVDSNIKGDKITYLVKEQKMQAFSDKGKRV
                     TTVLVPSQLQDKNNKGQTPAQKKGN"
     gene            22083..22808
                     /gene="lptB"
                     /locus_tag="ACHKBL_000538"
     CDS             22083..22808
                     /gene="lptB"
                     /locus_tag="ACHKBL_000538"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HFO5817460.1"
                     /translation="MATLTAKNLAKAYKGRRVVEDVSLTVNSGEIVGLLGPNGAGKTT
                     TFYMVVGIVPRDAGNIIIDDDDISLLPLHARARRGIGYLPQEASIFRRLSVYDNLMAV
                     LQIRDDLSAEQREDRANELMEEFHIEHLRDSMGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKRIIEHLRDSGLGVLITDHNVRETLAVCERAYIVSQGHLIAH
                     GTPTEILQDEHVKRVYLGEDFRL"
     gene            22856..24289
                     /gene="rpoN"
                     /locus_tag="ACHKBL_000539"
     CDS             22856..24289
                     /gene="rpoN"
                     /locus_tag="ACHKBL_000539"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HFO5817461.1"
                     /translation="MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESN
                     PLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPEELPLDASWDTIYTAGTPSGTSG
                     DYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVPLED
                     ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARL
                     IISDHLDLLANHDFRTLMRVTRLKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDV
                     LVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIRSNLQDAKWLIKSLE
                     SRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHS
                     PRGIFELKYFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQG
                     IMVARRTVAKYRESLSIPPSNQRKQLV"
     gene            24312..24599
                     /gene="hpf"
                     /locus_tag="ACHKBL_000540"
     CDS             24312..24599
                     /gene="hpf"
                     /locus_tag="ACHKBL_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HFO5817462.1"
                     /translation="MQLNITGNNVEITEALREFVTAKFAKLEQYFDRINQVYVVLKVE
                     KVTHTSDATLHVNGGEIHASAEGQDMYAAIDGLIDKLARQLTKHKDKLKQH"
     gene            24717..25208
                     /gene="ptsN"
                     /locus_tag="ACHKBL_000541"
     CDS             24717..25208
                     /gene="ptsN"
                     /locus_tag="ACHKBL_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HFO5817463.1"
                     /translation="MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLS
                     LPPQVVFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQ
                     PVDLLFALLVPADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTEGTP
                     DEA"
     gene            25254..26108
                     /gene="rapZ"
                     /locus_tag="ACHKBL_000542"
     CDS             25254..26108
                     /gene="rapZ"
                     /locus_tag="ACHKBL_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HFO5817464.1"
                     /translation="MVLMIVSGRSGSGKSVALRALEDMGFYCVDNLPVVLLPDLARTL
                     ADREISAAVSIDVRNMPESPEIFEQAMSNLPDAFSPQLLFLDADRNTLIRRYSDTRRL
                     HPLSSKNLSLESAIDKESDLLEPLRSRADLIVDTSEMSVHELAEMLRTRLLGKREREL
                     TMVFESFGFKHGIPIDADYVFDVRFLPNPHWDPKLRPMTGLDKPVAAFLDRHTEVHNF
                     IYQTRSYLELWLPMLETNNRSYLTVAIGCTGGKHRSVYIAEQLADYFRSRGKNVQSRH
                     RTLEKRKP"
     gene            26105..26377
                     /gene="npr"
                     /locus_tag="ACHKBL_000543"
     CDS             26105..26377
                     /gene="npr"
                     /locus_tag="ACHKBL_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS phosphocarrier protein NPr"
                     /protein_id="HFO5817465.1"
                     /translation="MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEA
                     EANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALFNSGFDED"
     gene            26590..27222
                     /gene="yrbL"
                     /locus_tag="ACHKBL_000544"
     CDS             26590..27222
                     /gene="yrbL"
                     /locus_tag="ACHKBL_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP regulatory network protein YrbL"
                     /protein_id="HFO5817466.1"
                     /translation="MIRLSEQSPLGTGRHRKCYAHPEDAQRCIKIVYHRGDGGDKEIR
                     RELKYYAHLGRRLKDWSGIPRYHGTVETDCGTGYVYDVIADFDGKPSITLTEFAEQCR
                     YEEDIAQLRQLLKQLKRYLQDNRIVTMSLKPQNILCHRISESEVIPVVCDNIGESTLI
                     PLATWSKWCCLRKQERLWKRFIAQPALAIALQKDLQPRESKTLALTSREA"
     gene            complement(27219..27947)
                     /gene="mtgA"
                     /locus_tag="ACHKBL_000545"
     CDS             complement(27219..27947)
                     /gene="mtgA"
                     /locus_tag="ACHKBL_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HFO5817467.1"
                     /translation="MSKSRLTVFSFVRRFLLRLMVVLAVFWGGGIALFSVAPVPFSAV
                     MVERQVSAWLHGNFRYVAHSDWVSMDQISPWMGLAVIAAEDQKFPEHWGFDVASIEKA
                     LAHNERNENRIRGASTISQQTAKNLFLWDGRSWVRKGLEAGLTLGIETVWSKKRILTV
                     YLNIAEFGDGVFGVEAAAQRYFHKPASKLTRSEAALLAAVLPNPLRFKVSAPSGYVRS
                     RQAWILRQMYQLGGESFMQQHQLD"
     gene            complement(27944..28597)
                     /gene="elbB"
                     /locus_tag="ACHKBL_000546"
     CDS             complement(27944..28597)
                     /gene="elbB"
                     /locus_tag="ACHKBL_000546"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HFO5817468.1"
                     /translation="MKKIGVILSGCGVYDGSEIHEAVLTLLAISRSGAQAVCFAPDKQ
                     QVDVINHLTGEAMTETRNVLIEAARITRGEIRPLAQADAAELDALIVPGGFGAAKNLS
                     NFASLGSECTVDRELKALAQAMHQAGKPLGFMCIAPAMLPKIFDFPLRLTIGTDIDTA
                     EVLEEMGAEHVPCPVDDIVVDEDNKIVTTPAYMLAQNIAEAASGIDKLVSRVLVLAE"
     gene            complement(28827..31163)
                     /gene="arcB"
                     /locus_tag="ACHKBL_000547"
     CDS             complement(28827..31163)
                     /gene="arcB"
                     /locus_tag="ACHKBL_000547"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic respiration two-component sensor
                     histidine kinase ArcB"
                     /protein_id="HFO5817469.1"
                     /translation="MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTM
                     VLHGQVESIDVIRSIFFGLLITPWAVYFLSVVVEQLEESRQRLSRLVQKLEEMRERDL
                     SLNVQLKDNIAQLNQEIAVREKAEAELQETFGQLKIEIKEREETQIQLEQQSSFLRSF
                     LDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPADVYSPEAAAKVIETDEK
                     VFRHNVSLTYEQWLDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDAL
                     ERASRDKTTFISTISHELRTPLNGIVGLSRILLDTELTAEQEKYLKTIHVSAVTLGNI
                     FNDIIDMDKMERRKVQLDNQPVDFTSFLADLENLSALQAQQKGLRFNLEPTLPLPHQV
                     ITDGTRLRQILWNLISNAVKFTQQGQVTVRVRYDEGDMLHFEVEDSGIGIPQDELDKI
                     FAMYYQVKDSHGGKPATGTGIGLAVSRRLAKNMGGDITVTSEQGKGSTFTLTIHAPSV
                     AEEVDDAFDEDDMPLPALNVLLVEDIELNVIVARSVLEKLGNSVDVAMTGKAALEMFK
                     PGEYDLVLLDIQLPDMTGLDISRELTKRYPREDLPPLVALTANVLKDKQEYLNAGMDD
                     VLSKPLSVPALTAMIKKFWDTQDDEESTVTTEENSKSEALLDIPMLEQYLALVGPKLI
                     TDGLAVFEKMMPGYVSVLESNLTAQDKKGIVEEGHKIKGAAGSVGLRHLQQLGQQIQS
                     PDLPAWEDNVGEWIEEMKEEWRHDVEVLKAWVAKATKK"
     gene            complement(31259..32188)
                     /gene="yhcC"
                     /locus_tag="ACHKBL_000548"
     CDS             complement(31259..32188)
                     /gene="yhcC"
                     /locus_tag="ACHKBL_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562163.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HFO5817470.1"
                     /translation="MQLQKLVNMFGGDLTRRYGQKVHKLTLHGGFSCPNRDGTIGRGG
                     CTFCNVASFADEAQQHRSIAEQLAHQANLVNRAKRYLAYFQAYTSTFAEVQVLRSMYQ
                     QAVSQANIVGLCVGTRPDCVPDAVLDLLCEYKDQGYEVWLELGLQTAHDKTLHRINRG
                     HDFACYQRTTQLARQRGLKVCSHLIVGLPGEGQTECLQTLERVVETGVDGIKLHPLHI
                     VKGSIMAKAWEAGRLNGIELEDYTLTAGEMIRHTPPEVIYHRISASARRPTLLAPLWC
                     ENRWTGMVELDRYLNEHGVQGSALGRPWLPPTA"
     gene            32863..37323
                     /gene="gltB"
                     /locus_tag="ACHKBL_000549"
     CDS             32863..37323
                     /gene="gltB"
                     /locus_tag="ACHKBL_000549"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HFO5817471.1"
                     /translation="MLYDKSLERDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGA
                     ILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAKNYAVGMLFLNKDPELAAAARRIV
                     EEELQRETLSIVGWRDVPTNEGVLGEIALSSLPRIEQIFVNAPAGWRPRDMERRLFIA
                     RRRIEKRLEADKDFYVCSLSNLVNIYKGLCMPADLPRFYLDLADLRLESAICLFHQRF
                     STNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLIPDLHDAAPFVNET
                     GSDSSSMDNMLELLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWD
                     GPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKGRVG
                     PGELMVIDTRSGRILHSAETDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSRELD
                     DDTLASYQKQFNYSAEELDSVIRVLGENGQEAVGSMGDDTPFAVLSSQPRIIYDYFRQ
                     QFAQVTNPPIDPLREAHVMSLATSIGREMNVFCEAEGQAHRLSFKSPILLYSDFKQLT
                     TMKEDHYRADTLDITFDVTKTTLEATVKELCDKAEKMVRSGTVLLVLSDRNIAKDRLP
                     VPAPMAVGAIQTRLVEQSLRCDANIIVETASARDPHHFAVLLGFGATAIYPYLAYETL
                     GRLVDTHAIAKDYRTVMLNYRNGINKGLYKIMSKMGISTIASYRCSKLFEAVGLHDDV
                     VGLCFQGAVSRIGGASFEDFQQDLLNLSKRAWLARKPISQGGLLKYVHGGEYHAYNPD
                     VVRTLQQAVQSGEYSDYQEYAKLVNERPATTLRDLLAITPGENAVNIADVEPASELFK
                     RFDTAAMSIGALSPEAHEALAEAMNSIGGNSNSGEGGEDPARYGTNKVSRIKQVASGR
                     FGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLISPPPHH
                     DIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADLITIAGYDGGT
                     GASPLSSVKYAGCPWELGLVETQQALVANGLRHKIRLQVDGGLKTGVDIIKAAILGAE
                     SFGFGTGPMVALGCKYLRICHLNNCATGVATQDDKLRKNHYHGLPFKVTNYFEFIARE
                     TRELMAQLGVTRLVDLIGRTDLLKELDGFTAKQQKLALSKLLETAEPHAGKALYCTEN
                     NPPFDNGLLNAQLLQQAKPFVDERQSKTFWFDIRNTDRSVGASLSGYIAQTHGDQGLA
                     ADPIKAYFNGTAGQSFGVWNAGGVELYLTGDANDYVGKGMAGGLIAIRPPVGSAFRSH
                     EASIIGNTCLYGATGGRLYAAGRAGERFGVRNSGAITVVEGIGDNGCEYMTGGIVCIL
                     GKTGVNFGAGMTGGFAYVLDESGDFRKRVNPELVEVLSVDDLAIHEEHLRGLITEHVQ
                     HTGSQRGEEILANWSTFATKFALVKPKSSDVKALLGHRSRSAAELRVQAQ"
     gene            37336..38754
                     /gene="gltD"
                     /locus_tag="ACHKBL_000550"
     CDS             37336..38754
                     /gene="gltD"
                     /locus_tag="ACHKBL_000550"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit GltD"
                     /protein_id="HFO5817472.1"
                     /translation="MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQA
                     DRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEAAELSHQTNTLPEVCGRVCPQDR
                     LCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDLSGVKQTGKKVAIIGAGPAGLA
                     CADVLTRNGVKAVVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTE
                     VGRDVQLDDLLSDYDAVFLGVGTYQSMRGGLENEDADGVYAALPFLIANTKQLMGFGE
                     TRDEPFVSMEGKRVVVLGGGDTAMDCVRTSVRQGAKHVTCAYRRDEENMPGSRREVKN
                     AREEGVEFKFNVQPLGIEVNGNGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHIVPA
                     DAVIMAFGFRPHNMEWLAKHSVELDSQGRIIAPEDNDNAFQTSNPKIFAGGDIVRGSD
                     LVVTAIAEGRKAADGIMNWLEV"
     gene            38937..40064
                     /gene="yhcG"
                     /locus_tag="ACHKBL_000551"
     CDS             38937..40064
                     /gene="yhcG"
                     /locus_tag="ACHKBL_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcG family protein"
                     /protein_id="HFO5817473.1"
                     /translation="MESLSERTSTGYQQIHDGIIHLVDSARTETVRSVNALMTATYWE
                     IGRRIVEFEQGGEARAAYGAQLIKRLSKDLSLRYKRGFSAKNLRQMRLFYLFFQHVEI
                     RQTVSGELTPLGIPQTPSAEFPSAKIWQTLSAKSFPLPWSTYVRLLSVKNPDARSFYE
                     KETLRCGWSVRQLERQITTQFYERTLLSHDKSAMLQQHAPAEMHILPQQAIRDPFVLE
                     FLELKDEYSESDFEEALINHLMDFMLELGDDFAFVGRQRRLRIDDNWFRVDLLFFHRR
                     LRCLLIVDLKVGKFSYSDAGQMNMYLNYAKEHWTLPDENPPIGLVLCAEKGVGEAHYA
                     LAGLPNTVLASEYKMQLPDEKRLADELVRTQAVLEEGYRRR"
     gene            complement(40124..40588)
                     /gene="nanQ"
                     /locus_tag="ACHKBL_000552"
     CDS             complement(40124..40588)
                     /gene="nanQ"
                     /locus_tag="ACHKBL_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006974 - DNA damage response [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate anomerase"
                     /protein_id="HFO5817474.1"
                     /translation="MIMGEVQSLPSAGLHPALQDALTLALAARPQEKAPGRYELQCDN
                     IFMNVMTFNTQSPVEKKAELHEQYIDIQLLLNGEERILFGTAGTARQCEEFHHEDDYQ
                     LCSAIENEQAIILKPGMFAVFMPGEPHKPGCVVGEPGEIKKVVVKVKADLMA"
     gene            complement(40585..41460)
                     /gene="nanK"
                     /locus_tag="ACHKBL_000553"
     CDS             complement(40585..41460)
                     /gene="nanK"
                     /locus_tag="ACHKBL_000553"
                     /EC_number="2.7.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HFO5817475.1"
                     /translation="MTTLAIDIGGTKLAAALIGADGQIRDRRELPTPASQTPEALHDA
                     LSALVSPLQVHAQRVAIASTGIIRDGSLLALNPHNLGGLLHFPLVKTLEQLTDLPTIA
                     INDAQAAAWAEYQALEGDITEMVFITVSTGVGGGVVSGGKLLTGPGGLAGHIGHTLAD
                     PHGPVCGCGRTGCVEAIASGRGIAAAAQGELAGADARTIFTRAGQGDEQAQQLIHCSA
                     HVLARLIADIKATTDCQCVVVGGSVGLAEGYLALVETYLAQEPAAFHVDLLAAHYRHD
                     AGLLGAALLAQGEKL"
     gene            complement(41457..42146)
                     /gene="nanE"
                     /locus_tag="ACHKBL_000554"
     CDS             complement(41457..42146)
                     /gene="nanE"
                     /locus_tag="ACHKBL_000554"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFO5817476.1"
                     /translation="MSLLAQLDQKIAANGGLIVSCQPVPDSPLDKPEIVAAMALAAEQ
                     AGAVAIRIEGVANLQATRAVVSVPIIGIVKRDLEDSPVRITAYIEDVDALAQAGADII
                     AIDGTDRPRPVPVETLLARIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTP
                     ETPEEPDLALVKTLSDAGCRVIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQ
                     WYNTAMKKAVL"
     gene            complement(42194..43684)
                     /gene="nanT"
                     /locus_tag="ACHKBL_000555"
     CDS             complement(42194..43684)
                     /gene="nanT"
                     /locus_tag="ACHKBL_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid transporter NanT"
                     /protein_id="HFO5817477.1"
                     /translation="MSTTTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLT
                     EVQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGDRYGRRLAMVTSIVLFSAGTLAC
                     GFAPGYITMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVA
                     AQVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHGGKAPVRTMVDILY
                     RGEHRIANIVMTLAAATALWFCFAGNLQNAAIVAVLGLLCAAIFISFMVQSTGKRWPT
                     GVMLMVVVLFAFLYSWPIQALLPTYLKTDLAYDPHTVANVLFFSGFGAAVGCCVGGFL
                     GDWLGTRKAYVCSLLASQLLIIPVFAIGGANVWVLGLLLFFQQMLGQGIAGILPKLIG
                     GYFDTDQRAAGLGFTYNVGALGGALAPIIGALIAQRLDLGTALASLSFSLTFVVILLI
                     GLDMPSRVQRWLRPEALRTHDAIDGKPFSGAVPFGRAKNDLVKTKS"
     gene            complement(43794..44687)
                     /gene="nanA"
                     /locus_tag="ACHKBL_000556"
     CDS             complement(43794..44687)
                     /gene="nanA"
                     /locus_tag="ACHKBL_000556"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008747 - N-acetylneuraminate lyase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HFO5817478.1"
                     /translation="MATNLRGVMAALLTPFDQQQALDKASLRRLVQFNIQQGIDGLYV
                     GGSTGEAFVQSLSEREQVLEIVAEEAKGKIKLIAHVGCVSTAESQQLAASAKRYGFDA
                     VSAVTPFYYPFSFEEHCDHYRAIIDSADGLPMVVYNIPALSGVKLTLDQINTLVTLPG
                     VGALKQTSGDLYQMEQIRREHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYQ
                     GIVKALKEGDIQTAQKLQTECNKVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGP
                     VDEKYLPELKALAQQLMQERG"
     gene            complement(44809..45600)
                     /gene="nanR"
                     /locus_tag="ACHKBL_000557"
     CDS             complement(44809..45600)
                     /gene="nanR"
                     /locus_tag="ACHKBL_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312126.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HFO5817479.1"
                     /translation="MGLMNAFDSQTEDSSPAIGRNLRSRPLARKKLSEMVEEELEQMI
                     RRREFGEGEQLPSERELMAFFNVGRPSVREALAALKRKGLVQINNGERARVSRPSADT
                     IIGELSGMAKDFLSHPGGIAHFEQLRLFFESSLVRYAAEHATDEQIDLLAKALEINSQ
                     SLDNNAAFIRSDVDFHRVLAEIPGNPIFMAIHVALLDWLIAARPTVADQALHEHNNVS
                     YQQHIAIVDAIRRHDPDEADRALQSHLNSVSATWHAFGQTTNKKK"
BASE COUNT        11191 a        12429 c        11413 g        10655 t
ORIGIN      
        1 gttagttttt tctttttaaa tgatgcggcc attgttctca gtaattaaat aacattatca
       61 atgcgtctct aaacattacg cagaacgtag ccatcagtag ttatactgac aggagcagga
      121 tggtttatcg agaagtaaac atatccgtat tcgtaccgga actggagttg acacaataaa
      181 gtgccaatta tgtcagcaga agggaaaagt ttataactaa ggcgtaccga taccatcgtt
      241 tcaaagtacc agctacgagt aaagcaactg gacgagatac agatacctga caaccattcc
      301 tcagaccagg accaaaacga aaaaagacgc ttttcagcgt ctcttttctg gaatttggta
      361 ccgaggacgg gacttgaacc cgtaagccct attgggcact accacctcaa ggtagcgtgt
      421 ctaccaattc caccacctcg gcacggatac tactttttag ttcgggatat cgctggtcgg
      481 cttagccgga gctgctggct gaatttgttc ggttttcgcc ggcgcactca ggttttccca
      541 ttcgctacct ttattggttt tgttgctatt gatgttaccc agcaccagac tgatgatgaa
      601 gaataacgtt gccagcagcg ctgtcatgcg ggtcatgaag ttaccagaac cacttgaacc
      661 aaacagcgta gcggaagcgc ctgctccgaa ggaggctccc atatcagcgc ctttaccttg
      721 ctgcagcatg atcagaccaa caaggccaat tgccacaata aggaaaacta ctaaaagagc
      781 ttcgtacata atcaacctgt tccttgcgga gttgccgcgt accaatgctt caaccaataa
      841 agcgggagtt tttaatcttt cccactgaag cgggtgtgaa tactaaccaa agcgaatgac
      901 cttcgcaagg gcaattttat tgcattgtat caactgcgga aaaaaacagc aaaaccgatg
      961 tgttcgctga gaaaaaaggc gacaatcgcc gcctttttag ccagttattt atcgttttaa
     1021 acggctttta ctgcatcggc gatgcggtgt gcaaattcag tcacctgcgc ttcgtcttcg
     1081 ccttccacca tcacgcgaat taacggttcg gtgccggatt tacgcaacag cacacgtcca
     1141 cggctaccca gcgcagcttc aacttctgcg gtcacagctt tgactgactc atgctccagc
     1201 ggatcgccgc tgcctgctgt gtaacgcacg ttaaccagaa tctgcgggaa cattttcatg
     1261 ccgctgcaaa ggtcgtgcag gctcatatgg ttacgcgcca tcgccgccag cacctgcaag
     1321 ccagcaacaa tgccgtcacc ggtagtggtt ttatccagca ggatcacatg accggagttc
     1381 tctgcaccga tacgccagcc tttctcctgc attttttcca gtacgtagcg gtcacccact
     1441 ttcgcgcgcg caaatggaat acctaactgt ttcagcgcca gttcaagccc catgttgctc
     1501 atcaatgtac ccacagcacc accacgcagc tggccctgac gaagaccttc acgcgcgatg
     1561 atatacatga tctgatcgcc atcgacttta ttgccttcat ggtcaaccat aatcacgcga
     1621 tcgccgtcgc cgtcgaaggc aataccgaga tccgcttttt cagccagcac acgagcctgg
     1681 agcgcgcgaa cgtcggtagc ccccacttcg gcattgatgt ttacaccgtt cggctcacaa
     1741 ccgatagcga taacgttcgc ccccagttcg cgcagcacgt tcggcgcgat gtgataagtc
     1801 gcaccgtttg cacaatccac cacaatcttc agttcactga ggctaagttc gttcgggaac
     1861 gtggctttgc aaaactcgat atagcgaccc gcagcatcaa cgatacggct ggctttaccc
     1921 agttctgccg aatcaacgca gctgatctct ttttccattt ccgcttcgat ggcctcttct
     1981 accgcatccg gtagtttggt gccgtcgata gagaagaatt taatgccgtt atcgtagaat
     2041 gggttatgcg atgcagaaat tacaattcca gcctctgcgc ggaaggtacg cgtcagataa
     2101 gccacggccg gtgttggcat cgggccagtg aagagcgcgg aaagaccggc tgccgcaagc
     2161 cccgcttcca gtgctgactc cagcatatag ccagaaatac gcgtgtcttt accaataata
     2221 atcttacggg agccgtggcg cgccagcact ttacccgcgg cccaacccag cttaagcaca
     2281 aaatcagggg tgatcggcgc atcccctaca cgaccacgaa tcccatcggt accgaaatat
     2341 ttacgattac tcatagcgtt tgttttcctt tgcagacaga gtggcttcca ccacccgcat
     2401 cgcttctacg gtttctttga cgtcatgaac acgaatgatg tgcgcgcctt gcattgcggc
     2461 aatgaccgca caggccagac taccgctcaa acgctcggac ggccccacgt tcagcagctg
     2521 tccaatcatc gattttcgtg acatacccac caacagcggc aggttgaaat ggtgaaattc
     2581 agccaggcgc gccagtaatg aatagttatg ggagagattt ttaccgaaac cgaatccggg
     2641 gtcgagcaac aatttctctt ttgcgatacc cgcctgctcg caacgtgcta tttgctcaat
     2701 aaagtagcga ttcacttctg caaagacatc gtgatacttc ggggcttcct gcatggtttt
     2761 tggatttccc tgcatatgca tcagacaaac cggtaaaccg gtttctgcag ccgcctccag
     2821 agcgccaggt tcggaaaggg agcggatatc gttaataatg tgagcgccaa ctttcgctga
     2881 ctcacggatg acttctggtt tggatgtatc taccgagatc cagacttcga agcgttgagc
     2941 aattgcctca accacaggaa taacacgttg taactcttct tcaacgctaa cttccgccgc
     3001 ccctggacgt gtggactcgc caccaacgtc aatgatcgtc gcgccagcgt tgatcatcag
     3061 attcgcatgt ttcaccgcat ctatcagcga gttatgcgtg ccaccatccg aaaaggaatc
     3121 aggcgtgacg ttgaggatcc ccattacgtg aggatggcta aggtccagtg aagtaccctg
     3181 ggcaaagagt ttcataatgt tatccctggt atgaattgat aagaaaaaac cccggagcac
     3241 gccccggggt ttttggtaca aatacagtca tctgatgcgg gaacttactt gtcgcctaac
     3301 tgctctgaca tggtgttacc cgggttcggc gtacgcggtt catcaaccgg acgaggagcc
     3361 tttggactac cattgtcgcc agaattgtta gaagcgcctg gttcttccca gcccgctggc
     3421 ggacgtacat cgcgacgtgc catcaggtca tcaatctgcg gtgcgtcgat agtctcatat
     3481 ttcatgagag catctttcat cgcatgcagt atatccatat tgtcggtcag aagctgacgc
     3541 gcacgattat agttgcgctc aatcagtgct ttcacttcct ggtcgatgat acgtgcagtt
     3601 tcatcggaca tatgtttcgc cttcgctacg ctacggccga ggaacacttc accttcttct
     3661 tccgcgtaca gcagtggacc caatttctca gagaagcccc actgagtcac catgttacgt
     3721 gccaggttgg tcgcaacttt aatatcgttg gacgcaccgg tagaaacatg ttccggcccg
     3781 tagatgatct cttctgccag acgaccaccg tacagcgtag aaatctggct ttccagtttc
     3841 tgacggctag cgctgattgc gtcgccctca ggcaagaaga aagtcacacc cagcgcacga
     3901 ccgcgtggga taatcgtcac tttgtgcacc ggatcatgtt ccggcaccag gcgaccgata
     3961 atcgcatgac ccgcttcgtg gtaagccgtc gattctttct gcgcttccgt catcaccatg
     4021 gagcgacgtt ccgcacccat catgattttg tctttcgctt tctcgaactc aaccatcgac
     4081 acaacgcgtt tgttgccacg agcagcgaac agtgccgctt cgttcaccag gttcgccagg
     4141 tcagcaccgg agaaaccagg agtaccacgg gcaatgattg ccgcgtcgat atcgggtgcc
     4201 aatggtacgc gacgcatgtg aactttcagg atctgttcac gaccgcgaac atctggcaag
     4261 ccaaccacaa cctgacggtc gaaacggcca ggacgcagca gcgccgggtc gagaacgtcc
     4321 ggacggttag tcgcggcgat aacgatgata ccttcgttac cttcgaagcc atccatctca
     4381 accagcatct ggttcagagt ctgttcacgt tcatcgtgac caccgcccag acctgcgcca
     4441 cgctggcggc ctacggcgtc gatttcatcg ataaagatga tgcacggtgc cgctttcttc
     4501 gcctgttcga acatgtcacg aacacgggat gcacccacac cgacgaacat ttctacgaag
     4561 tcagaaccgg agatagtaaa gaacggaact ttcgcttcgc ctgcaatcgc tttcgccagc
     4621 aacgttttac cggtacccgg aggaccgacc atcaggacgc ctttcgggat cttaccgccg
     4681 agtttctgga agcggctcgg ctcgcgcaga tactcaacca gttcagcaac ttcttctttt
     4741 gcttcgtcgc agcccgcaac gtcagcaaag gtcgttttga tctgatcttc agtcagcatg
     4801 cgcgctttgc tcttaccaaa cgacatggca cctttgccac cgccgccctg catttgacgc
     4861 atgaagaaga tccagacacc aatcagcaac agcatcggga accaggagat gaagatagaa
     4921 gccagcaggc ttggttcttc aggcggttca ccgacaacct tgacgttctt ggtcaacaag
     4981 ttatccagta atttcggatc ctgaaccgga atgtaagtgg tataacggtt actatctttc
     5041 ttggtaacgt tgatttcacg tccgttgata cgcgcttcac gaacctggtc gttattgacc
     5101 tcttgtagga aggtagagta atccacctta cggccattag actcgctggg cccaaagctc
     5161 tggaatactg acatcagcac aacggcaatg accagccaga gtattaggtt tttcgccatg
     5221 tcactcaagg gattaacctc ttattacaac tgtgttaaaa acagcgtcag gatactctat
     5281 atccagcatc tttcaaactt tcgtctgaaa tctcccggtt agggtttacg cccggtcgct
     5341 acaatataca cttcccgcga acgtgcgcga gaagagtccg gcttacgaac tttgaccttc
     5401 gtaaacaggg agcgaatttc ccttagatac tcatcgaaac cttcgccctg gaacaccttc
     5461 actacaaaac tgccacctgg cgctaataca tcacgacaca tttctagcgc cagttccacc
     5521 agatacatgg cacgggggat atccaccgcc ggtgttccgc tcatgtttgg tgccatatcg
     5581 gacatgacaa cctggacttt gctgtcgcca acgcgctcca gcagtgcttt catcaccagt
     5641 tcatcacgaa aatcgccctg aagaaagtcc acaccaacga taggatccat aggtaaaaga
     5701 tcgcaagcga tgatacggcc tttaccgcca atttgggtga ccacatattg tgaccaacca
     5761 cccggcgcag caccaaggtc gacaaccgtc attcccggtt taaagagttt gtcactttgc
     5821 tgtatttcat caagtttaaa ccaggcacgg gaacgtaacc cctttttctg tgcctgttga
     5881 acatatttat cgctaaagtg ttcctgaagc cagcggctgg agctggcaga acgcttctta
     5941 cctgtcattt aactttccca ttgggataac tcatcgtaac caattgcgta aatttttacg
     6001 cgcctatttg gcgatatatg ggagatggcg gtagaatgac ccgttttcaa tcccaacgta
     6061 agcaaaaaat acgatgaatc tgagtactaa acaaaaacag cacctgaaag gtctggcaca
     6121 tccgctcaag ccagttgttc tgcttggcag taatggtttg accgaagggg tgctggccga
     6181 gattgaacaa gcgttagagc accatgaact catcaaggtg aaaatcgcca ccgaagatcg
     6241 cgaaactaaa accttgatcg tggaagctat cgtgcgcgaa accggcgcct gtaatgtaca
     6301 ggtcatcggt aaaacgctgg tgctttatcg cccaactaaa gaacgtaaaa tctcgctgcc
     6361 acgctaagat tatcctaaag ttacacacat ttgctgtgta aaacgagggg ttttccgcag
     6421 gcaggagagc aaaatgccac gctctgttcg ttgataaaag gccgcatagc ggcctttttc
     6481 ctttctttac aatacatcaa catcttgagt attgggtaat tcttacaggt attccacctt
     6541 aattacttca aattctactt cgccgcccgg cgttttgatg accacaacat catcttcttc
     6601 tttgccgatc aggccacgag caataggcga gtttacagaa atcaggtttt gtttaaagtc
     6661 agcttcgtca tcgccaacga tgcgataagt ctgttcttcg tcagaatcca gattcagcac
     6721 cgttacggta gcaccaaaaa taacgcgccc attgttgggc attttggtga catcaatcac
     6781 ctgcgcgttc gacagcttgg cttcgatgtc tttgatacgg ccttcgcaga agccctgctg
     6841 ttcacgagct gcgtggtatt cggcgttttc tttcaggtcg ccatgctcac gcgcttccgc
     6901 gatagcagca atgatttcag gacggcgcac agatttcaga aaatccagct cttcgcgtaa
     6961 tttttcagcg ccgcgtaagg tcatcggaat agcttgcatt tgttatacct cttgaatatt
     7021 cctgatgggg caagtcttca cccaccccgg ctgttcggcc tgcccggcat aactcctcac
     7081 cgggaccaga agcaaaaaaa taccgacccg ggtacaagtc ccaggtcagc tacaattcac
     7141 attttgatag tcattttacc ctgaagttcc cgaagggtca tcgtttactt tatagggcgt
     7201 tgcgccgtag tatgacggct cgattccagg ttgttagcgc gagattatgc gattttccag
     7261 atttatcatc ggattgacca gctgtatagc gttcagtgtt caggccgcaa atgttgatga
     7321 gtacattact caactccccg ctggtgccaa ccttgccctg atggtgcaaa aagtcggcgc
     7381 gtcggccccc gctattgatt accacagtca gcagatggcg ctgcccgcca gtacgcagaa
     7441 agtgattact gcactggcgg cgttgattca actcggcccc gattttcgtt ttaccacgac
     7501 gcttgaaacc aaaggcaatg tggaaaacgg cgtacttaag ggtgacttag tggcgcgatt
     7561 tggtgccgat ccgacgttaa gacgtcagga tattcgcaat atggtcgcga ctttgaaaaa
     7621 atctggcgtc aaccaaatcg atggcaatgt gttgatagat acctccattt tcgccagcca
     7681 cgataaagcc cccggctggc catggaatga catgacacaa tgctttagcg ctccgcctgc
     7741 cgccgccata gttgaccgca actgtttctc cgtctcgctc tatagtgccc caaaacctgg
     7801 tgatatggct tttatacgcg tggcatctta ttaccccgtt acgatgttca gccaggtacg
     7861 caccctcccc cgtggttctg ccgaagcgca atactgcgag ctggatgtgg tgccaggcga
     7921 cctgaaccgc tttacgctga ccggatgcct gccacaacgt tctgagccgc tcccgttggc
     7981 ttttgccgtg caggatggtg ccagctatgc tggagcaatt ctgaaagatg agttaaaaca
     8041 ggcaggtatc acctggagcg gaacactgct gcgtcagacg caggttaacg aacccggaac
     8101 ggtagttgcc agtaaacagt cggccccgtt gcacgatctg cttaagatta tgctgaaaaa
     8161 gtcggacaac atgattgccg atacggtttt ccgcatgatc ggccatgcgc gcttcaatgt
     8221 gcctggaaca tggcgggccg ggtcggacgc cgtgcgtcag atcctgcgcc agcaagccgg
     8281 tgtcgatatt ggaaatacca ttattgccga tggttcaggg ctttcgcggc ataacctgat
     8341 tgctcccgcc accatgatgc aggtgctgca atacattgcc caacacgaca atgaacttaa
     8401 ctttatctct atgctgccgc tggcgggcta tgacggctct ttgcagtacc gtgcaggtct
     8461 gcatcaggcg ggcgtggatg gaaaagtctc agcgaaaacc ggttcgttgc agggggtata
     8521 taacctggcg ggattcatta ccacagcgag cgggcaacga atggcgtttg tgcaatatct
     8581 ttctggctat gcagtagaac cggcggatca gcgtaatcgc cgtattccgt tggtacgttt
     8641 tgaaagccgt ttgtataagg atatttacca gaacaattag tctaaagaga aaccccggca
     8701 catggccggg gttttgctga ttaacgcttg taaatgaact caacgccttc ttcgtcgtct
     8761 tcgtcccagt cgtcatccca gtcttcatca tcctcttcag caatctcttc aagctgctgg
     8821 cgatgataat catcccacat gaattcgact ttctctggct gtttcgcttc ttcagcctgc
     8881 acgaccgggt tttcaatgat aaaggtcatc acatcccagc agagatcttt cacgcccagt
     8941 ccgctcgccg cagagatcag ataatattta tcttcccaac ccagcgcctc agcgatcgct
     9001 ttcgctttct cttcggcttc tgccttatcc agcaggtcga tcttgttgaa gactaaccaa
     9061 cgcggtttcg ccgccagatc ctggctgtat ttttccagct cgctgataat aatacgcgcg
     9121 ttttcaaccg gatcggtgcc atcaatcgga tcgatatcga tgaggtgcaa caggacgcgg
     9181 caacgctcca ggtgcttcag gaagcgaatg cccagacctg cgccttctgc agcgccttca
     9241 atcagtcctg gaatatcggc aacaacgaag ctcttttcgt tgtccattcg taccacacca
     9301 agacttggca ccagagtggt aaacggataa tccgccactt tcggtttagc cgccgatact
     9361 gcgcggataa atgtcgattt acccgcgttt ggcatcccca gcataccgac gtcagccagc
     9421 agcatcagct ccagtagcag ctcacgctta tcgcccggcg taccgttagt tttctgccgt
     9481 ggtgtacggt taactgacga tttgaaacgg gtattgccca gaccgtgcca gccgccctta
     9541 gcaaccagca gacgctgacc gtgtttggtc atatcgccca tggtttcacc ggtaccctgg
     9601 tcgataacac gcgtacctac cggcacttta atcgtcacgt ctttaccgcg cttaccggta
     9661 cagtcgcggc ttgcgccatt ctgaccacgc tctgcgcgga aagatttttc aaaacgataa
     9721 tcgataagcg tgttcaggtt ctcgtcggct tccatccata cgtcaccgcc gtcaccgccg
     9781 tcgccgccat ccgggccgcc tttcggaata tacttttcgc ggcggaagct cacgcaacca
     9841 ttaccgccat cacctgcaac gaccagaatc gatgcttcat caacaaactt cattttattc
     9901 tccgtaaatc attcgcctgc gcggggttgc gataccaccg ttgtatgctt acgtaatccg
     9961 ccccaaatac gatgaccaat ggcggaatac atcgcgcccg caaccacgac aaacgcaccg
    10021 agataaccta aaaggtttaa catcggtctg gcgaagaaat cgggccaggc cagtgataaa
    10081 agatctgaaa aaaacagcgt aaacagtggg gtgagcgtaa tgatcgcgct cacctgcgct
    10141 gcctgccagc gagccatcgc ttccgccagg gcgccatatc ctaccaatgt attcagtccg
    10201 caaaaaatta aacatgcgag ctgccagtgg ctaagctgcg ctatcactcc aggctttgcc
    10261 agagggaaga gcgcaattgt acataaagtg tacagcaaaa acaggatctg cggtgaggcc
    10321 agtcgacgca ataaaacctt ttgcgccacg ccataactca cccacaccgt cgccgcaccg
    10381 accccaaaga ttactcccca ggtgtaatcg gtgagctttg taaatatctc gaccagactg
    10441 gtgttaaaaa acatcaccag gccgctcagg agcatcaatg cccctacaac ctgagtgctg
    10501 cgcattttct ctttcaggat aaatacgctg gcaaccatca tgccaactgg cgagagttgc
    10561 ccaatcacct gcgaagcggt cggacccagg tattgcaagg atgagctgaa caggatgaag
    10621 ttcccaaaca gcccggcggt cgccactgcc aacaaaatca accagcgtgg cttacgaaac
    10681 acgcgtaatg gcggcaaccg cttcttcacc gcaagaatgg cacccaggcc aatactcgcc
    10741 atcaagaaac ggtaaaacac gattgtcgga ggttccatca cctccagcac ctgcttcatt
    10801 gcgattggca acgcccccca gcaaattgct gtggtgagcg ccaaaagaat gccaatgcct
    10861 gcctgctgct tcataccgta ttccctgcaa agcgcatttg cggattaacg tcgcaccatg
    10921 cgcattttta ccggttatcg aatgtaaaaa gccccgcaac gtgttgcggg gctttcatcc
    10981 gttaccggga cgcgaaaaac ttattcagct tcgatgctga taaatttacg gtttttcggg
    11041 cctttaactt cgaatttcac tttaccgtct gctttagcaa acagagtgtg gtcacgaccg
    11101 caacctacgt tagcgccagc gtggaatttg gtaccacgtt gacgaacgat gatgctaccc
    11161 gccagaacgg attcgccacc gaaacgctta acgcccaggc gtttagcttc tgaatcgcga
    11221 ccgttacgtg tggagccgcc agccttttta tgtgccattt gaaatctctc ctcaggtctt
    11281 aggcgctgat gccagtaatt ttcacatcag tgaaccactg acgatggccc tgctgcttac
    11341 gatagtgttt acgacgacga aacttaacga ttttaacttt ctcgccacga ccgtgagcaa
    11401 caacttcagc tttgattacg ccgccatcaa cgaaaggaac gccgattttg acttcttcac
    11461 cgtttgcgat catcagcact tcagcgaact caacagtttc gccagttgcg atgtccagct
    11521 tttccaggcg aacggtctga ccttcgctta ctcggtgttg tttaccacca ctttggaaaa
    11581 ccgcgtacat ataaaactcc gcttccgcgc acaccttttc aatgattcag agtgcgctat
    11641 aaatattcac aatagggcgc gaatattacg caaaacgcac gcctttgaca agtgctacag
    11701 tcaatacacg aagaaaaaaa acacaacttg tacggtaacg tttatctgtg ccattttttc
    11761 agtacaatca ccctatattc ctaaccataa accctaagtt gcctttgttc acagtaaggt
    11821 aatcggggcg aaaagcccgg cttttgcgat gaatttagaa aaaatcaatg agttaaccgc
    11881 gcaagatatg gcgggtgtta atgcggcaat ccttgagcag cttaattccg acgtccaact
    11941 gatcaatcag ttaggctatt acatcgtcag cggcggcggt aaacgtattc gtccgatgat
    12001 tgctgtactg gctgcacgag ctgttggcta tgagggaaat gcgcatgtca ccattgctgc
    12061 cctgatcgag tttatccaca cggcgactct gctgcacgac gacgttgtgg atgaatcaga
    12121 tatgcgcagg ggtaaagcta ccgccaacgc tgcatttggc aatgccgcca gcgtgctggt
    12181 aggcgatttt atttataccc gcgcgttcca gatgatgacc agcctcggct cgctcaaagt
    12241 gctggaagtc atgtcagaag ccgtaaacgt catcgcagaa ggtgaagttc tgcaactgat
    12301 gaacgttaac gatccggaca tcactgaaga aaactacatg cgcgttatct atagcaaaac
    12361 cgcgcgtctg tttgaggctg ccgcgcagtg ttccgggatt ctggctggct gtacgccgga
    12421 ggaggagaaa ggcctgcagg attatgggcg ctatctcggc actgctttcc agttgatcga
    12481 cgatttactc gattacaatg ccgatggcga acagttaggt aaaaacgtcg gcgacgatct
    12541 gaacgaaggt aaaccgacgc tgccgctgct gcatgcaatg catcatggca caccagaaca
    12601 ggcacagatg attcgtaccg ccatcgaaca gggtaacggt cgccatcttc tggaaccggt
    12661 tctggaagca atgaacgctt gtggatctct tgaatggacg cgtcagcgtg ccgaggaaga
    12721 agcagacaaa gccatcgcag cgttacaggt gctcccggac accccgtggc gagaagcact
    12781 catcggcctc gcgcacatcg ctgttcaacg cgatcgttaa tcccctcccc tcatcccgcg
    12841 cagcgcgcgg gatgattcca gtaaattcca taaaaacact tattcagctc tataacacct
    12901 gaaaaatagg gacgtacatc tttatatatt ctgaatattc acactcttta caggaacttt
    12961 ttagagcaat aggccatcag gagtatagtg atgctcgaca gaagaagtgt tctgaatgaa
    13021 agcgactaac ttaaggagtg aggaaagatg gaaagtaatt tcattgactg gcatcccgct
    13081 gacatcattg cgggtttgcg caagaaggga acgtcaatgg cggcggaatc tcgcagaaat
    13141 ggtttgagtt cctcaacgct ggcgaacgca ttatcgcgcc cctggccgaa aggagagatg
    13201 attattgcga aagccctggg aactgacccc tggattatct ggccatctcg ctaccatgat
    13261 ccgcagaccc atgagtttat cgacagaacg cagttgatgc gtagctacac taaaccgaaa
    13321 aaatgagtgg tctggcggta gccccgcgaa cggggctgcc agctctcaga caattactcg
    13381 cctttcacac gctcaatatt tgcacctaaa gcgcgcagtt tgtcttcaat gcgttcgtag
    13441 ccacgatcga tgtgataaat acgatcaact accgtcgtcc cttccgcaat acagccagcc
    13501 agcaccaggc ttgctgatgc acgcagatcg gttgccataa cctgtgcgcc agaaagtttt
    13561 tcaacaccgt gacaaataac ggtattgctt tcgatttcgg cgtgcgcgcc catacggctc
    13621 agctctggca catgcataaa gcggttttca aagaccgttt cggtgatgaa gccggtccct
    13681 tctgccacca ggttcaacag cgtgaactgg gcctgcatat cggtcgggaa tgccggatgc
    13741 ggcgcggtac gtacgttaac agccttcgga cgtttgccat gcatatccag gctaatccag
    13801 tcttcgccga cttcgatgtc tgctccagcg tcacgcagtt tcgccagcac ggcgtcgaga
    13861 gtatctggct gcgcgttacg gcagataatt ttgccgcgag agatcgccgc cgccaccagg
    13921 aaagtaccgg tttcgatacg atccggcagc acgcgataga caccgccgcc taaacgttcc
    13981 acaccttcga tgacgatacg atcggtgccc tgaccgctaa ttttcgcacc cagcgtaatc
    14041 aggaagttcg cggtatcgac gatttccggt tcacgcgctg cgttttcaat aatcgtggtg
    14101 ccttccgcca gggttgcagc acacatgatg gtcaccgttg cgccaacgct gactttatcc
    14161 atcacgatat gtgcgccttt cagacgacca tcgacggaag ctttaacgta accttcttcc
    14221 agtttgatgg tcgcgcctaa ttgttcgaga ccagaaatgt gtagatcaac cggacgtgca
    14281 ccaatcgtac aaccgccagg cagtgaaact tgcccctgac caaagcgcgc taccagcggc
    14341 cccagcgccc agatagaagc acgcatggtt ttaaccagat cgtaaggtgc gcagaataca
    14401 ttaacgtcgc gggcatcaat atgcacagaa ccattacgtt ctactttcgc acccagctgg
    14461 cttagcagct tcattgatgt atcgacgtct tttagtttcg ggacgttctg gatctctacc
    14521 ggttcttccg ccagtagtgc ggcaaaaagg ataggcagag cagcattttt agcgccggaa
    14581 attgtgactt cgccctggag cttcgttggc ccctgaacac gaaatttatc catttagttt
    14641 gttctcagtt aacaattcat atccgctacc ggcgaatcgc ccatagctca aaagccgttc
    14701 agtttgcgat cgcgcgccca ctccgcaggg gtatacgctt tgatcgacac agcatgaatg
    14761 cggttatccg caatatattc catcagcggg ccatagaccg tctgctgttt tttaacccga
    14821 ctcatgccgt caaacaactc acccacggca ataacctgaa agtggctgcc atcgccggaa
    14881 acgtggactt cctggaggga gagagcgttc atcaacacgc tctgaatttc attattttcc
    14941 atgggatctt caatcatcag ttaataaacc agcgaaacat cttagagcaa agttgcgctg
    15001 gcataaataa gcaaaaagcc tcgctgataa atcagacaag gctcgacttg caggcaggtt
    15061 tgccggacag gcggttaacg ccatatccgg cctgaaaaaa tttaacgagg cagaacatca
    15121 gcaggcaaat tatacaattt cgccagggta tacactttgt cgtttacccc ctgaagcgtc
    15181 acattgttgc cctgcttttt cgccagatcg ataagatgga gcagcagcgc cagtccacct
    15241 gtatcaacgc gggatacacg gctaagatcg atacaggtaa tccccttcac cgcttcctca
    15301 cgcatttccc aaagcggtag cagaacgtcc tgatccagct ctccggataa cgccagcgtg
    15361 tcacccgtct gcatccagct cagtgactcg ctcattattt tttctcttcc agagtgattt
    15421 tctgttgaga aatcgatttc agttgcgcag tcaggccgtc aatacctttg gtacgcagca
    15481 gcgttcccca ctcgttttgt ttggtggtga tcatactgac gccttcagca atcatgtcgt
    15541 aagcctgcca gttgcccgtc tgggagtttt tacgccactg gaagtccaga cgcaccggcg
    15601 ggcggccatt cgggtcaata atggtaacgc gaataggcac aatggtttta tcacccagcg
    15661 gctgttctgg cgcaatctga taggtttgac cgtgatacat cgccagcgcc tgaccgtaag
    15721 cctgcttcag gtactcacgg aaagcggcaa agtaagcatc acgttgagct ggggtcgcac
    15781 tcttgtaata ctgacccagc accagcgcac cggcgtattt cacctgtacg tatggcagca
    15841 gttcctgatc aacaatggta cgcagataat ccgggttggc ccgaatttgt ggttgctcat
    15901 tcttcaggcg atcgaacgtt ttctgcgccg cctcgtccat cagcttatac ggattggtct
    15961 ggtctgccgc ggttgccgca ctcagcggtg caatcaccag caaagcgacc atcattaaac
    16021 gtttaaacat gcgtcggttc tcctgaaatt atttcgttgt acccacaggt tcagtggttt
    16081 cattattacc tggcgcagca gctggcgcat cgccactatt cttattgtca tcgcctttac
    16141 taccgtaaag gaactgacca ataagatctt ccagcaccat cgcagacttg gtgtcctgaa
    16201 ttgtatcgcc atccttcagg atagcagtcc ccagttccgg gtcttcaaaa ccgacgttta
    16261 atgccagata ttgttccccc agcaggccgg aagtacgaat gctcagcgaa ctggtatcag
    16321 gaatgtggtt ataacgttgt tcaatttcca gcgttacgcg cggcagatag gttttcgggt
    16381 ccagcgtaat atccgccacc cggcccacaa caacaccacc aatgctgacc ggagagcgcg
    16441 ctttcaggcc gccaatgtta tcgaacgtcg cataaagcgt gtaggtcggt tcagtacgta
    16501 tggacgtcac gttcgccgcc ttcaggcaaa caaacagcgc cgccagtagt gctgctaata
    16561 aaaagatacc cacccaaatt tcattttttt tcgtttgcat gaactcaatt cccaaacatc
    16621 aatgcggtca gcacaaaatc cagccccaga acagccagag acgagtggac aacggtgcga
    16681 gtggttgccc ggctaatccc ggcagacgtc gggatggcgt cgtagccgtt aaacaacgaa
    16741 atccacgtca ccgtgatggc aaacaccacg ctcttaatca gacagttgac cagatccata
    16801 cgccagtcga cggcattttg cattgccgac cagaagaacc cgctatcaat gcctttccaa
    16861 ctgacgccga ccagagatcc gccccagatc cccacggcga cgaaaataac cgtcaacagt
    16921 ggtaatgaaa taaccccagc ccagaaacgg ggagaaataa cccgacgcag cggatccacc
    16981 gccatcatct ccatactgga gagttgctct gtagcgcgca tcaggccgat ttctgcggtt
    17041 agcgccgaac cagcacgccc ggcaaacaac aacgcggcaa ccaccggccc cagttcacgc
    17101 agtagcgata acgccaccag catacccaga ctggtttccg cactataagt ggtcagaacc
    17161 agataacctt gcagccccaa caccattccg atgaacacgc cagaaaccac aataatcagc
    17221 atcgacagga cgccgacatt atagagctgg cgcaccagca gcggcgcatg tttgcgaaat
    17281 tccggtttgc cgaccagcgc attgaataac attaacccgg cccgcccgaa cgttctcagg
    17341 gttttaatcc ctttatgtcc gagcgacgcc agcgcattta acagcatgag tggcttaact
    17401 ccctggtaaa agatcagcgt gataatcgcc ggcaggatag cggaacggaa caggcccgtc
    17461 agctatcccg tccagaaact gacgtacgcg tggatcagga ttcgcctgca acgcctgggc
    17521 actgccatga gcgacaattt ttttgtccgc caggatccag gcgtgatcgg caatacttaa
    17581 cacttccggc acatcgtgag aaaccaccac acaagtcaca cccagcgcgc tgttcagctc
    17641 agaaatcagc ttcaccagta cgcccatggt gatgggatct tgcccaacaa agggttcatc
    17701 aaacatgatg agatccggct ccagcgcaat cgcacgcgcc agcgctgcac gccgcgccat
    17761 cccaccggaa agttcagaag gcattagttt agccgctcca cgcagcccca cggcctccag
    17821 cttcatcatc accgtactat gcaacaatgg cgcgggaagt tgggtatgtt cgcgcagtgg
    17881 ataggcgacg ttgtcaaata cgttcatatc agtgaacaac gcccctgact gaaataacat
    17941 gctcatccgt ttgcgcactg tatacaggcg cgaacgagac atcgccggaa tattctcacc
    18001 atcgaaaaga atctcaccat gatctggtgc gatttgcccg ccaatcagac ggagtagcgt
    18061 cgttttaccg atgcccgatg gccccatgat cgccgtgatc ttccctcgcg gcacggtcag
    18121 ggaaatatta tcgaagatgc agcgattgcc acgcgtaaaa ctgacatcgc gcatatcgac
    18181 taaattcgcc acagactgct ccataattca cccttcgtct tgcgttgatt ttctaagcat
    18241 ggcgctcaat ttaaccttga acccaacata tttacagaat attacccgcc gtggttagcg
    18301 aaagctggca tttgttttac tttttagccg cataaagtca aaattaagaa tccgttacgg
    18361 ctttctgaaa atcttcagcg gaccggcgag tatacctgaa gaaaggacgt tagatgcttt
    18421 tagctacggc actgttaatt gttggtttac ttttggtcgt ttacagtgcc gaccgcctgg
    18481 tttttgccgc gtctattctt tgccgaacct ttggcatccc gccgctaatc atcggcatga
    18541 cggtggtcag tattggtaca tcgttaccag aaatcatcgt ctcgcttgcc gcgtctctgc
    18601 acgaacaacg cgatttagcc gttggtacag ccctcggctc aaacattatc aatatattgc
    18661 tgatcctcgg tctggctgcg ctggttcgtc cttttaccgt ccattctgat gttctacgcc
    18721 gtgaattacc cttaatgttg ttggtcagcg tggtggccgg ttccgtactc tatgacggac
    18781 aacttagtcg aagcgatggt atctttctcc tctttctggc tgtgctatgg ctgctgttca
    18841 ttgttaaact tgcacgtcag gctgaacgtc aggggactga cagcctgacc agagagcagc
    18901 ttgcagagct gcctcgtgac ggcggattgc ccgtcgcgtt tttatggctc ggcattgcgc
    18961 ttatcatcat gccagtggcc acgcggatgg tggttgataa cgccacggtg ctggcgaatt
    19021 actttgccat cagcgagttg acgatgggtc tgacggcaat tgctatcgga accagcctgc
    19081 cggaactggc aaccgcaata gcgggggttc gcaaaggtga aaacgacatt gctgtcggaa
    19141 atatcattgg cgcaaacatt tttaatattg tcatcgtatt gggtttaccc gcgctgataa
    19201 cgccaggaga gattgatcca ctggcgtaca gtcgtgacta cagcgtgatg ttgctggtga
    19261 gcattatttt tgcgttgctg tgctggcggc gctccccgca accgggccgt ggtgtagggg
    19321 tattattaac tggcggattt atcgtatggc tggcgatgtt gtactggtta tcgccaatac
    19381 tcgttgaata actggaaacg cattatgtcg cacgtagagt tacaaccggg ttttgacttt
    19441 cagcaagcag gtaaagaagt cctggcgatt gaacgtgaat gcctggcgga gcttgatcaa
    19501 tacatcaatc agaatttcac gcttgcctgt gaaaagatgt tctggtgtaa agggaaagtt
    19561 gtcgtcatgg ggatggggaa atcggggcac atcgggcgca aaatggccgc aacgtttgcc
    19621 agcaccggta caccttcatt tttcgtccat cctggtgaag ccgcgcatgg tgatttaggc
    19681 atggtcaccc cacaggatgt ggtgattgct atctctaact caggtgaatc cagcgaaatc
    19741 acggccttaa ttccagtgct taagcgtctt cacgtaccgt taatctgcat caccggtcgc
    19801 ccggagagca gcatggcgcg cgccgcagat gtgcatctgt gtgttaaagt agcgaaagaa
    19861 gcctgtccgt tagggctggc accgaccagc agcaccaccg ccacgctggt tatgggcgat
    19921 gccctcgctg tcgcgctgtt aaaagcacgc ggctttactg ctgaagattt tgcgctctca
    19981 cacccaggcg gcgcactggg tcgtaaactt ctgctgcgcg taaacgatat tatgcatacg
    20041 ggcgatgaga tcccgcatgt taagaaaacg gccagtctgc gtgacgcatt gctggaagtt
    20101 acccgcaaaa atcttggtgt gactgtcatt tgcgatgaca atatgatgat tgaaggcatc
    20161 tttaccgacg gtgatttacg ccgtgtcttc gatatgggcg tggatgttcg tcagttaagt
    20221 attgccgatg tgatgacgcc ggggggaata cgtgtgcgcc ctggcattct ggccgttgag
    20281 gcactgaact taatgcagtc ccgccatatc acctccgtga tggttgccga tggcgaccat
    20341 ttactcggtg tgttacatat gcatgattta ctgcgtgcag gcgtagtgta aagattcaag
    20401 gataaacaac aatgagcaaa gcaggtgcgt cgcttgcgac ctgttacggc cctgtcagcg
    20461 ccgacgttat agcaaaagca gagaacattc gtctgctgat cctcgatgtc gatggcgtac
    20521 tgtcagatgg cctgatttat atgggcaata atggcgaaga gctgaaagcg ttcaatgttc
    20581 gtgacggtta tggcattcgt tgtgcgctca cctctgatat tgaagtcgct atcattaccg
    20641 ggcgaaaggc taaactggta gaagatcgtt gtgccacatt ggggatcact cacttgtatc
    20701 aggggcagtc aaacaaactg atcgccttta gcgatttgct ggaaaaactg gcgattgccc
    20761 cggaaaatgt ggcttatgtc ggcgatgatc tcatcgactg gccggtaatg gaaaaagtgg
    20821 gtttaagcat cgccgtggcc gatgcgcatc cactgttaat cccgcgcgcc gattacgtga
    20881 cgcgcattgc tggcggtcgt ggtgcagtgc gtgaagtttg cgacttatta ctcctggcgc
    20941 agggcaaact ggatgaagcc aaagggcaat cgatatgagt aaagccagac gttgggttat
    21001 cattgtgctg tcactggcgg ttctggtgat gatcggcatt aatatggccg aaaaagacga
    21061 taccgcccag gtggtcgtca acaacaatga tcccacctat aaaagcgagc atacggacac
    21121 gctcgtctat aacccagaag gggcactaag ctatcgattg attgctcaac acgttgaata
    21181 ttattccgat caggccgttt cgtggtttac gcagccggta cttaccacgt ttgataagga
    21241 taaaatcccg acatggtccg taaaagcaga taaagccaag ctgaccaatg accggatgct
    21301 ctatttatat ggacacgttg aagtcaacgc actcgtgccg gactctcaac ttcgcagaat
    21361 cacgacggat aacgcgcaga tcaatctggt gacgcaggat gttacctctg aagacctcgt
    21421 cacgttatac ggaacaacat ttaactccag cggtctgaaa atgcgcggca acttacgcag
    21481 caagaacgcc gagctgattg aaaaggttag aacatcctat gaaattcaaa acaaacaaac
    21541 tcagccttaa tcttgtgctt gccagctcac ttctggccgc cagcattccg gcatttgccg
    21601 taaccggaga cactgatcag ccgatccaca ttgaatcgga ccagcaatct cttgatatgc
    21661 aaggcaacgt ggttaccttt accggcaatg tcatcgtcac ccagggcacc atcaaaatta
    21721 atgccgacaa agtggtcgtt acccgtccgg gcggcgaaca aggtaaagaa gtgattgacg
    21781 gctacggtaa accggcaacg ttctaccaga tgcaggacaa cggtaaaccc gttgaaggtc
    21841 acgcttcaca gatgcactac gaactggcaa aagattttgt cgttctgacg ggtaatgctt
    21901 atctgcagca ggtcgatagc aacattaagg gcgataagat cacttacctg gtgaaagagc
    21961 agaaaatgca ggctttcagc gacaaaggca agcgcgtaac caccgttctg gtgccgtcgc
    22021 agctgcagga caaaaacaat aaaggccaga ccccggcaca gaagaagggt aattaattcg
    22081 ttatggcaac attaactgca aagaaccttg caaaagccta taaaggccgt cgcgtggtag
    22141 aagacgtcag cctgaccgtc aactccgggg aaattgtcgg tctgctgggg ccaaacggtg
    22201 ccggtaagac caccactttc tacatggttg ttggcattgt gccgcgcgat gcgggcaaca
    22261 tcattattga tgatgacgat atcagtctgc tgcctctgca tgcacgcgcg cgccgcggta
    22321 tcggctatct gccgcaggaa gcctccattt tccgtcgcct cagcgtttac gataacctga
    22381 tggcggtact gcaaattcgt gacgacttgt ctgctgaaca acgtgaagac cgcgcgaacg
    22441 agctgatgga agagtttcac attgagcacc tgcgtgacag catggggcag tcactctccg
    22501 ggggtgaacg tcgccgtgta gaaattgccc gtgcactggc tgcgaatccg aaatttattc
    22561 tgctcgacga accgtttgcc ggggttgacc cgatctcggt tatcgacatt aaacgcatca
    22621 ttgagcacct gcgcgacagc ggcctgggcg tactgatcac tgaccacaac gtgcgtgaaa
    22681 cactggcggt ttgtgaacgc gcttatatcg tcagtcaggg gcatttgatc gcccacggca
    22741 cgcctacaga aatcttacaa gacgaacacg ttaagcgtgt ataccttggg gaagacttca
    22801 gactctgata gggtagaagt ttgcgatgtt ttagcaggag agtacgattc tgaacatgaa
    22861 gcaaggtttg caactcaggc ttagccaaca actggcgatg acgccacagc tccaacaggc
    22921 aattcgtctg ttgcagttgt cgacgctgga acttcagcag gagctacagc aggcgctgga
    22981 gagtaatccg ctgcttgagc aaatcgacac tcatgaagaa atcgacaccc gcgaaacgca
    23041 agacagtgaa acactggaca ccgccgacgc gctcgaacaa aaagagatgc cggaagagct
    23101 gccgctcgat gccagttggg acaccattta caccgctggt acaccatccg gcaccagcgg
    23161 tgactacatt gacgacgagc tgccggtcta tcagggcgaa acgacgcaga ccttgcagga
    23221 ttacctgatg tggcaggtcg agctgacacc gttttccgac actgaccgcg ctattgctac
    23281 ctctatcgtc gatgccgttg atgacaccgg ttatctgact gtcccgctgg aagatattct
    23341 cgaaagtatg ggcgatgaag agattgacat cgacgaagtt gaagccgtcc ttaagcggat
    23401 ccaacggttt gatccggtcg gtgtggcggc aaaagatctg cgtgactgtc tgctgatcca
    23461 actctcccaa ttcgataaaa ccacgccatg gctggaagag gccagactga tcattagcga
    23521 tcatctcgat ctgttagcca atcacgactt ccgcacttta atgcgcgtca cgcggctgaa
    23581 agaagatgtg ctgaaagaag ccgtcaatct gatccagtcg ctcgatccgc gccccgggca
    23641 gtcgatccag acgggcgaac ctgagtatgt cattccagat gtgctggtgc gtaagcataa
    23701 cggtcactgg acggtagaac tcaacagtga cagcattccg cgtctgcaaa tcaaccagca
    23761 ctacgcctcg atgtgcaata acgcgcgcaa cgatggtgac agccagttta tccgcagcaa
    23821 tctgcaggat gccaaatggt tgatcaagag cctggaaagc cgtaacgata cgctactgcg
    23881 cgtgagtcgc tgtatcgttg aacagcagca agccttcttt gagcaaggcg aagaatatat
    23941 gaaaccgatg gtactggccg atatcgccca ggctgtcgaa atgcatgaat cgacgatatc
    24001 tcgcgtgacc acgcaaaaat acctgcatag tccacgaggc atttttgaac tgaagtattt
    24061 cttttccagt cacgtcaata ccgagggcgg cggcgaagct tcctccacgg cgattcgtgc
    24121 gctggtgaag aaattaatcg cggcggaaaa cccagcgaaa ccgttgagcg acagcaagtt
    24181 aacctctttg ctgtcggaac aaggtatcat ggtggcacgc cgcactgttg cgaagtaccg
    24241 agagtcttta tccattccgc cgtcaaacca gcgtaaacag ctcgtttgac ccaaccgata
    24301 aggaagacac tatgcagctc aacattaccg gaaataacgt cgagatcacc gaggcactgc
    24361 gcgaatttgt tacagccaaa tttgccaaac ttgagcaata ttttgaccga atcaaccagg
    24421 tctatgttgt tctgaaagtg gagaaagtca cccacacctc agatgcaaca ctgcatgtaa
    24481 acggtggtga aattcatgcc agcgcggaag gtcaggacat gtacgctgcc attgatggct
    24541 taattgataa gctggcacgc cagttgacca aacataaaga taaactgaaa caacactaat
    24601 tgtccgggca attagcatgt gcatggcggc ctgttgtgcg gcacaacggg ccatttgtac
    24661 ggttaatgct ccgagcctgt tccactgttt gagtgggcag gttcttaggt gaaattatga
    24721 caaataatga tacaactcta cagcttagca gtgttcttaa cagggaatgt acgcgaagcc
    24781 gcgtccactg tcagagcaaa aaacgcgccc tggaaatcat cagcgagctg gcggcgaaac
    24841 aacttagtct gccgcctcag gtggtttttg aagctatcct gacgcgggaa aaaatgggca
    24901 gtaccggtat cggcaatggt attgccattc cgcatggcaa actggaagaa gatactctgc
    24961 gcgccgttgg cgttttcgtt cagctcgaaa cgcctatagc tttcgatgcc attgacaacc
    25021 aaccggtaga tttactattt gcgttgctgg ttccggctga ccaaaccaaa acgcacctgc
    25081 atactctgtc gctggtggcg aaacgtctgg cggacaaaac catctgccgc cgtttacgcg
    25141 cagcccagag cgatgaagag ctgtatcaaa tcattacgga taccgaaggt actccggatg
    25201 aagcgtagtt attcggtaat gtctctttta gacgttgtga ggagaaacag tacatggtac
    25261 tgatgatcgt cagcggacgt tcaggttcag gtaaatctgt cgccctgcgt gcgctggaag
    25321 atatgggttt ttactgcgtg gataaccttc ccgtagtgtt gttacccgat ctggctcgaa
    25381 ccctggccga tcgcgagatt tctgccgccg tcagcattga tgttcgtaat atgccggagt
    25441 caccagaaat attcgaacag gcgatgagta acctgcctga cgctttctca ccgcaactcc
    25501 tgttcctgga tgccgaccgt aataccttaa ttcgtcgtta cagtgacacg cgccgactgc
    25561 atccgctttc cagcaaaaac ctgtcgctgg aaagtgctat cgacaaagaa agcgatttgc
    25621 tggagcctct gcgttcgcga gcggatctga ttgtcgatac ctcagaaatg tccgttcacg
    25681 agctggcaga aatgctgcgt acccgtctgc tgggtaaacg tgaacgtgaa ctgaccatgg
    25741 tctttgagtc tttcggcttc aaacacggta tccctatcga tgcagattac gtctttgacg
    25801 tgcgcttctt gccgaacccg cactgggatc cgaaactgcg tccaatgaca ggtcttgata
    25861 aacctgtcgc cgcgttcctc gaccgccaca cagaagtaca caattttatc taccagacgc
    25921 gaagctatct tgagctatgg ttacctatgc tggaaaccaa caaccgtagc tacctgacgg
    25981 tcgccattgg ttgtaccggc gggaagcacc gttcggtgta tattgcagag caactggcag
    26041 actacttccg ctcgcgcggt aaaaacgtcc agtcacgcca tcgtacgctg gaaaaacgta
    26101 aaccatgacc gtcaagcaaa ctgttgaaat cacaaacaag ctgggcatgc atgcccggcc
    26161 tgcaatgaag ctgtttgaat taatgcaagg ttttgacgca gaagtgctgt tacgtaatga
    26221 cgaaggtacc gaggccgaag ccaacagcgt tattgcactg ctgatgctgg attctgccaa
    26281 aggacggcag attgaagttg aagcgaccgg tccacaggaa gaggaagcac tggccgccgt
    26341 tatcgccctc tttaattctg gttttgatga agattaatct tcatcacttt gacattcaaa
    26401 tcccttcaag ctcccccgac aataagaaaa gcgcgtgctg aaatcgttct caatcaacgt
    26461 catttgtaca ttttgtgcgc ttttcacttt cagaagaacc ttaagaaaac cttaagaagc
    26521 attgtttagg tttcgtttaa gttaatcgac catactggag atcgtcagaa aatatttcca
    26581 ggagatggca tgattcgctt atctgaacaa agtcccctgg gcaccggaag acatcggaaa
    26641 tgttatgcgc atccggaaga tgcccaacgc tgtatcaaga ttgtctacca tcgtggcgat
    26701 ggtggcgata aagagatccg ccgtgagtta aagtactacg cgcatcttgg tcgccggtta
    26761 aaagactgga gtggaatacc gcgctatcac ggtaccgttg agacggattg cggaaccggt
    26821 tatgtctacg atgtgattgc agattttgac ggcaaaccct ccattacgct gaccgaattt
    26881 gctgaacaat gccgttacga agaagacatt gcacaactgc gccagctact gaaacagctg
    26941 aagcgttatt tgcaggataa ccgtatcgtg acgatgtcgc tgaagccgca gaatatcctc
    27001 tgtcaccgca tcagtgagtc agaggttatc cccgtcgtct gcgacaacat tggtgaaagc
    27061 acattgattc cactggcgac ctggtcgaag tggtgttgtc tgcgtaagca agagcggtta
    27121 tggaaacgat ttatcgccca accggcgctg gctattgccc tgcagaaaga tctgcaacct
    27181 cgtgagagca aaacgctggc cctcacttcg cgcgaagctt aatccagctg gtgctgttgc
    27241 ataaacgact caccgcctaa ctgatacatc tgccgtaaaa tccacgcctg acggctacgc
    27301 acgtagcccg atggcgcgga gactttgaaa cgaagtggat taggtaatac agcagccagt
    27361 aatgcagctt ccgagcgggt aagtttgctc gcgggtttgt ggaaataacg ttgtgccgca
    27421 gcttcgacgc caaacacacc gtcgccaaat tcggcgatat tcaggtaaac cgtcaggata
    27481 cgctttttgc tccagactgt ttctatcccc agcgttaatc cagcttccag cccttttcgt
    27541 acccagctac gcccatccca taaaaagaga tttttggccg tctgctgaga aatcgttgaa
    27601 gcaccgcgaa tacggttttc attgcgctcg ttgtgcgcca gggctttctc aatggaagcg
    27661 acatcaaagc cccagtgctc aggaaatttc tgatcttctg cggcaatcac cgccagtccc
    27721 atccacggcg agatttgatc catactgacc cagtcagaat gtgccacgta acgaaaattg
    27781 ccatgcagcc aggcgctgac ctgtcgctcg accattaccg ctgagaaggg aacaggcgca
    27841 acactaaaca acgcgatgcc cccgccccag aaaacggcga ggacaaccat taaccgcaaa
    27901 agaaaacggc gaacgaaact aaacaccgtt aagcggcttt tactcattca gccagaacca
    27961 gcacgcggga aaccagctta tcaatgccgc tcgccgcttc tgcaatgttc tgcgccagca
    28021 tatatgctgg ggtggtgaca atcttattgt cttcatcaac cacgatatca tcgacaggac
    28081 atggcacatg ctccgcgccc atctcttcca gcacttctgc ggtatcgata tcagtaccga
    28141 tggtcaaacg cagcgggaaa tcgaaaattt tcggcagcat cgccggggca atacacataa
    28201 aaccaagcgg ttttccggcc tgatgcatcg cttgtgccag cgcctttaat tcacggtcaa
    28261 cggtgcattc gctaccaaga ctggcaaaat tgcttaaatt cttcgccgcg ccaaaacccc
    28321 ccggcacaat caacgcatcc agttcagcgg catcggcctg ggccagagga cggatttcac
    28381 cgcgcgttat tcgtgccgct tcaatcagca cattgcgcgt ttccgtcatc gcttcgcccg
    28441 ttaaatggtt gataacatca acctgctgct tatccggtgc aaagcagacc gcctgcgcac
    28501 cgctgcgtga aatagctaac aacgtcaaca ccgcttcatg aatttcagaa ccgtcataga
    28561 cgccgcatcc gctcagaatt acgccaattt tcttcattgt gatcatcctt tcgcaattga
    28621 ctgaaacaca ttaatttttt taataaaaat ggtacgcatc acacatttaa ctgattcatg
    28681 taacaaatca tttaagtttt gctatcttaa ctgcgtgcgg cctgaaaaac agtgctgtgc
    28741 ccttgtaact catcataata atttacggcg cagccaagat ttccctggtg ttggcgcagt
    28801 attcgcgcac cccggtctag ccggggtcat tttttagtgg cttttgccac ccacgctttc
    28861 agcacttcta cgtcgtgacg ccactcttct ttcatctctt caatccattc accgacgtta
    28921 tcttcccagg caggaaggtc aggagactga atttgctgac ccagctgttg cagatggcgt
    28981 aaccccactg accccgccgc acctttaatt ttatgtcctt cctcaacaat gcctttttta
    29041 tcctgcgccg tcagattcga ctccagcacg ctgacatagc ccggcatcat cttctcaaac
    29101 accgctaacc cgtcggtgat cagcttcggt cctacaagtg cgagatactg ttccagcatg
    29161 ggaatatcga gcaatgcttc tgatttactg ttctcttctg tcgtcaccgt actctcctca
    29221 tcatcctggg tatcccagaa tttcttgatc atcgcggtta gcgccggaac cgaaagcggc
    29281 ttactcagca catcatccat tccagcattg aggtactctt gtttgtcttt cagcacgtta
    29341 gcggttaagg ccaccagcgg cggtaaatcc tcgcgcggat aacgcttcgt cagttcacga
    29401 gagatatcca gcccggtcat atctggcaac tgaatatcca gcaacaccag gtcgtattcg
    29461 cccggtttaa acatctccag cgccgccttg ccggtcatgg cgacatcaac gctgttacct
    29521 aatttttcca gcacagaacg cgcaacaatc acgttcagtt caatgtcttc caccagcagc
    29581 acattcagcg ccggtaaagg catatcgtct tcatcaaacg catcatcgac ctcttctgct
    29641 accgacggtg cgtggatcgt caacgtaaag gttgaacctt tgccctgttc gctggtaacc
    29701 gtaatatcgc cgcccatatt tttcgccaga cgacgagaaa cggccagacc aataccggtg
    29761 ccggtggcag gtttaccgcc atgactgtct ttcacctggt aatacatggc gaaaatttta
    29821 tccagctcat cctgcggaat gccgatacca gagtcttcca cttcaaaatg cagcatatcg
    29881 ccttcatcgt agcgcacgcg cacggtaacc tggccttgct gggtgaattt gacggcgtta
    29941 ctgatgagat tccacaggat ctgccgtaaa cgcgtcccgt cggtaatgac ctgatgcggt
    30001 aatggcagcg taggctccag gttaaagcgc aatccttttt gttgcgcctg caatgcggaa
    30061 agattttcca gatcggcaag gaagctggtg aaatcaaccg gctgattatc aagctggacc
    30121 ttacgccgtt ccatcttatc catgtcgata atatcgttga agatattccc gagcgtgacg
    30181 gccgaaacat ggatggtttt gagatatttt tcctgctcgg cggtgagttc ggtatccagc
    30241 agaatgcggc tcaggccgac gataccattc agcggtgtac gcaattcgtg actgatggtg
    30301 gagataaacg tcgttttgtc gcggctggcc cgttcaagcg catcctgata ccgcttacgc
    30361 tcggtaatgt cgcgaccaaa gcccatcaaa ccgtgacgtt tacccacgcg gtcgtagtac
    30421 ggcactttac ggatttcaaa gcaggctttg cgtccgtcgg gataatccag ccactgctca
    30481 taggtcagtg acacattatg acggaacact ttttcatcgg tttcaatgac tttcgcagcg
    30541 gcttccggtg agtaaacatc agcaggtttc agatgaacca gttgtttttc gctttttccg
    30601 gtcagcagct ccatcgcgcg gttacagccg gaaaactctt tatcttcgtt acgataaaaa
    30661 accaggtcgg gtgaagcgtc aaggaaggaa cgtaagaatg aggattgctg ctcgagctga
    30721 atttgtgtct cttcgcgctc tttgatttca attttcagtt ggccgaaggt ttcctgcagt
    30781 tctgcttccg ctttttcacg aacggcgatt tcctgattta gctgggcaat attatctttt
    30841 aactgaacgt tgaggctcaa atcgcgctcg cgcatctcct ccagtttttg caccagtcgt
    30901 gacagacgtt gtcgtgactc ctccagttgc tcgacgacca ccgatagaaa gtagaccgcc
    30961 cacggcgtaa tcagcaaacc aaagaagata gaacgaataa catcaatgct ttcgacctga
    31021 ccatgcagca ccatggttac cgccatttgt accacaatgg caagaacgac gagggccagc
    31081 gccagtaaca ttgagaagcg caccagacct aacttcatca tcaggtcaac ataatactgc
    31141 gccagcagac gaatttgctt cattagggaa ttccttcacg acaacctgcc ccaataatac
    31201 ccaattctga cagttacgtt ataaattgtg tgagaaatgc ggaattcccc tcaatggcct
    31261 acgccgttgg aggtagccac ggacgtccca gcgccgatcc ctgtacgcca tgttcattca
    31321 gataacgatc cagctcgacc attcccgtcc atcgattttc gcaccacagc ggagcaagca
    31381 gcgtcgggcg acgggcgctg gcggaaatgc ggtggtagat cacttccggt ggcgtatggc
    31441 gaatcatttc tccggcagtg agcgtgtaat cctccagttc aattccgttc aaacgccccg
    31501 cttcccaggc tttcgccata atactgcctt tcacaatatg cagcggatgc agctttatgc
    31561 cgtccacgcc ggtttcaacc actcgctcca gcgtttgcaa acattccgtc tgcccttcgc
    31621 caggcaggcc gacaattaag tgggaacaca ccttcagccc gcgctgacgt gccagctggg
    31681 ttgtacgctg ataacaggca aaatcatggc cgcggttgat gcgatgcagc gttttgtcgt
    31741 gggcggtttg tagccccagc tccagccaca cttcgtaacc ctggtcctta tattcgcaaa
    31801 gcagatccag caccgcatcc ggcacgcagt ccgggcgggt accaacacac aaaccgacaa
    31861 tattggcctg gctcaccgcc tgctgataca tagaacgcag cacctgaact tccgcaaagg
    31921 tgctggtata cgcctgaaag taggccagat agcgtttagc gcggttaact aaattcgcct
    31981 ggtgcgccag ttgctcggca atggaacgat gctgctgcgc ttcatcggca aacgaggcca
    32041 cattacagaa tgtgcagcca ccacgcccga tggtaccgtc acggttaggg cagctaaaac
    32101 cgccgtgcag cgtcagctta tgcacctttt gcccataacg acgggtgaga tcaccaccaa
    32161 acatattgac taatttctgt aactgcataa tctgatagac cgcgccttga aaagaggcca
    32221 aagcctgcca tttttagccc aattcggcga tgacctggat caatcgtccc ggcccgcttt
    32281 tatcaactgc ataatcaatc aaaattaccg aaatttcatg cacaatcaca taaatcactt
    32341 ttgcttatct tgtgtcagat ttttttatct ccttatggat tttaggcaaa aacagtagca
    32401 tgaaacgtta ttaccaatta aggcagtata aaatgctggt tttctcgtca gttcaaggca
    32461 ggataagggt taacacgcct ttatgacagt caggaattgg ctgtttctct aacgacgccc
    32521 cttttagcct taaatagaaa atccacttta atttcagcca attaataaag aattttgcgc
    32581 taaagcacat ttctgtacca ataagcttgc catttgacct gtatcagctt tcccgataag
    32641 ttggaaatcc gctggaagct ttctggatga gcagcctgct catcatattt atgcagtaat
    32701 tgagatcccc tcttcaccgt attaaccgat gcgaaaagga caacaagggg gcgaatgcga
    32761 ggcgcgcgta tgacacgcaa accccgtcgc cacgctcttt ctgtgcccgt gcgcagcggt
    32821 tcggaagtgg ggttcccgca gagcctgggg gaggttcacg atatgttgta cgataaatcc
    32881 cttgagaggg ataactgtgg tttcggcctg atcgcccaca tagaaggcga acctagccac
    32941 aaggtagtgc gtactgcaat acacgcactg gcccgcatgc agcaccgtgg cgcgattctc
    33001 gccgatggta aaaccggtga cggttgcggc ttgctgttac aaaaaccgga tcgctttttt
    33061 cgcatcgttg cgcaggagcg cggctggcgt ttagcaaaaa actacgccgt cgggatgctc
    33121 ttcctgaata aagatcctga actcgccgct gccgcacgcc gcatcgttga agaagagctg
    33181 caacgcgaaa cgttgtcgat tgtgggctgg cgtgatgtcc ccactaacga aggcgtgctg
    33241 ggtgaaatcg ccctctcctc tctgccacgc attgagcaaa tttttgtgaa cgccccggca
    33301 ggctggcgtc cgcgcgatat ggagcgccgt ctgtttatcg cccgccgccg cattgaaaag
    33361 cgtctcgaag ccgacaaaga cttctacgtc tgtagcctgt cgaatctggt caatatttat
    33421 aaaggtctgt gtatgccggc ggatctgccg cgcttctacc tggacctcgc ggacctgcgt
    33481 ctggaatcgg ctatctgcct gtttcaccag cgcttttcca ctaacaccgt accgcgctgg
    33541 ccgctggcgc agccgttccg ctacctcgcg cataacggtg aaatcaacac catcaccggt
    33601 aaccgccaat gggcgcgtgc gcgtacctat aaattccaga cgccgctgat ccctgacctg
    33661 cacgacgccg cgccgttcgt caacgaaacc ggctctgact ccagttcgat ggataacatg
    33721 ctggaactcc tgctggcagg cgggatggat atcatccgtg ccatgcgtct gttagtgcca
    33781 ccagcctggc agaacaaccc ggatatggac ccggaactgc gtgccttctt tgactttaac
    33841 tccatgcata tggagccgtg ggatggtccg gcagggatcg tgatgtccga tggccgtttc
    33901 gccgcctgta acctcgaccg taacggtctg cgtccggcac gctacgtcat caccaaagat
    33961 aaactgatca cctgcgcctc tgaagtcggt atctgggatt atcagcctga cgaagtggtc
    34021 gaaaaaggcc gcgtagggcc aggcgaactg atggtgatcg acactcgcag tgggcgcatt
    34081 ctacactcgg cagaaaccga tgatgacctg aaaagccgcc atccctataa agagtggatg
    34141 gagaaaaacg tccgccgtct ggtaccgttt gaagatctgc ccgatgaaga agtgggtagc
    34201 cgcgaactgg acgacgacac gcttgccagc taccagaaac aatttaacta cagcgcggaa
    34261 gagctggatt cggtgatccg cgtactgggt gaaaacggtc aggaagcggt cggttcgatg
    34321 ggcgacgata ccccattcgc cgtgctctcc agccagccgc gcattattta cgactacttc
    34381 cgccagcagt ttgctcaggt aaccaacccg ccaatcgacc cgctgcgcga agcgcatgtt
    34441 atgtcgctcg ccaccagtat cggtcgtgaa atgaacgtct tctgtgaagc agaaggccag
    34501 gcgcaccgtt taagctttaa atcgccgatt ctgctctatt ccgatttcaa acagctcacg
    34561 acgatgaaag aggatcacta ccgcgcagat acgctggata tcacctttga cgtcactaaa
    34621 accacgctcg aagcgacggt gaaagagctg tgcgacaaag ccgaaaaaat ggtacgtagt
    34681 ggcaccgtgc tgctggtgct ctccgaccgg aatatcgcta aagatcgcct gccggttccg
    34741 gctccgatgg cggttggcgc gatccagacc cgtcttgtcg agcaaagcct gcgttgcgat
    34801 gccaacatca tcgtagaaac cgccagcgcc cgtgatccgc accacttcgc cgtgttgctg
    34861 ggtttcggcg cgacggctat ttatccatac cttgcctatg aaacgctggg ccgcctggta
    34921 gacacccacg cgattgccaa agattatcgt accgtgatgc tcaactatcg taacggcatc
    34981 aacaaaggct tgtacaaaat catgtccaaa atgggcatct ccaccatcgc ctcttaccgc
    35041 tgctcgaaac tgtttgaagc ggtcggtctg cacgatgatg tcgtgggcct gtgcttccag
    35101 ggggcggtta gccgcattgg gggggctagc tttgaagact tccagcagga tctgctgaat
    35161 ctgtcgaaac gagcctggct ggcgcgtaag cctatcagcc agggcggcct gctgaaatac
    35221 gtgcacggtg gcgaatacca cgcctacaat ccggacgtgg tgcgcacgct gcaacaggcg
    35281 gtacagagcg gcgagtatag cgactatcag gaatacgcga agctggttaa tgagcgtccg
    35341 gcaaccacgc tacgcgatct gctggcaatt acgccgggtg aaaacgcggt caacattgct
    35401 gatgttgaac cggcaagcga actgtttaaa cgcttcgata ccgccgcgat gtctatcggc
    35461 gcgttaagcc cggaagccca cgaggcgctg gcggaagcga tgaacagcat cggcggtaac
    35521 tcaaactccg gcgaaggcgg tgaagacccg gcgcgctatg gcactaacaa agtgtcgcgc
    35581 atcaagcagg tggcgtccgg tcgcttcggc gttactccgg cgtacctggt caatgccgac
    35641 gtcattcaga taaaagtcgc ccagggcgcg aagccgggtg aaggaggtca gttaccgggt
    35701 gataaagtca ctccttacat tgccaaactg cgctattcgg tgccgggcgt gacgctgatc
    35761 tccccgccgc cgcaccacga tatctactct attgaggatt tagcgcagct gattttcgac
    35821 ctcaagcagg ttaacccgaa agcgatgatc tccgtgaagc tggtttccga accgggcgta
    35881 ggcaccatcg cgactggcgt ggcaaaagct tatgccgatt taatcaccat cgcaggctat
    35941 gacggcggca ccggcgcaag tccgctttca tcggtgaaat acgcaggctg tccgtgggag
    36001 ctggggcttg ttgaaaccca acaggcgctg gttgccaacg gcttgcgtca caagatccgt
    36061 ttgcaggtcg atggcggcct gaaaacgggt gtcgatatca tcaaagcagc gattcttggc
    36121 gcggaaagtt ttggcttcgg cactggcccg atggtagcgc ttggctgtaa atatctgcga
    36181 atttgccacc tgaacaactg cgccacgggt gtagcaactc aggatgacaa actgcgtaag
    36241 aatcactatc acggcctgcc gttcaaagtg actaattact ttgagtttat cgcccgtgaa
    36301 acccgcgagc tgatggcaca gctgggggta acgcgtctgg tggatctgat tggtcgcacc
    36361 gacctgctga aagagctgga cggtttcacc gccaaacagc agaaactggc actgtcgaag
    36421 ctgctggaga ctgctgagcc gcatgctggt aaagcgctct actgcaccga aaacaacccg
    36481 ccgtttgata acggcctgct gaacgcgcag ttgctgcaac aggcaaaacc gtttgtcgat
    36541 gaacgccaga gcaaaacctt ctggttcgat atccgcaaca ccgaccgttc tgtcggcgca
    36601 tcgctttcag gctatatcgc ccagacgcac ggcgatcagg ggctggctgc cgatcctatc
    36661 aaagcgtact tcaacggcac cgcaggccag agcttcggcg tgtggaacgc gggcggcgtg
    36721 gaactgtacc tgaccggtga tgccaacgac tatgtcggta aaggcatggc gggcggctta
    36781 atcgccattc gtcctccggt tggctcagcc ttccgcagcc atgaagcgag cattatcggc
    36841 aacacctgcc tgtatggcgc gaccggtggt cgtctgtatg ccgcaggccg cgcgggtgaa
    36901 cgtttcggcg tgcgtaactc cggtgctatc accgtggtag aaggcattgg cgacaacggc
    36961 tgtgaatata tgacgggtgg tatcgtctgt attctgggta aaaccggcgt taacttcggc
    37021 gcaggcatga ctggcggctt cgcttacgtt ctcgatgaaa gcggcgattt ccgcaaacgc
    37081 gttaacccgg aactggtcga ggtcttaagc gttgacgatc tggcgatcca tgaagagcat
    37141 ctgcgcggtc ttatcaccga gcatgttcag cataccggtt ctcagcgcgg tgaagagatt
    37201 ctggcgaact ggtcaacctt cgccactaaa tttgcgctgg ttaaaccgaa gtccagtgat
    37261 gtaaaagcac tgctgggtca ccgtagtcgt agcgcagcag agttgcgcgt gcaggcgcag
    37321 taaggggtag caacaatgag tcagaatgtt tatcaattta tcgacctgca gcgcgttgat
    37381 ccgcctaaga aaccgctgaa gatccgcaaa attgagtttg ttgaaattta cgagccgttt
    37441 tccgaaggcc aggccaaagc gcaggctgac cgctgcctgt cgtgcggcaa cccatactgc
    37501 gagtggaaat gcccggtaca caactacatc ccgaactggc tgaagctcgc caacgagggg
    37561 cgtatttttg aagcggcgga actgtcgcac cagaccaaca ccctgccgga agtatgcgga
    37621 cgtgtctgcc cgcaagaccg tctgtgcgaa ggttcctgca ccctgaacga tgagtttggc
    37681 gctgtgacca tcggcaacat tgagcgttat atcaacgata aagcgttcga aatgggctgg
    37741 cgtccggatc tgtccggcgt gaaacagacc ggtaaaaaag tggcgattat cggcgctggc
    37801 ccggcgggtc tggcgtgtgc ggatgttctg acgcgcaacg gcgtaaaagc ggtcgtcttc
    37861 gaccgtcacc cggaaattgg cggattgctg accttcggta ttccggcttt caagctggaa
    37921 aaagaggtaa tgacgcgccg ccgtgaaatc ttcaccggca tgggtattga attcaaactc
    37981 aataccgaag tgggccgcga cgtgcagctg gacgatctgc tgagtgatta cgatgccgtg
    38041 ttccttggcg tcgggactta tcagtcaatg cgcggcgggc tggaaaacga agacgccgat
    38101 ggcgtgtacg cagcgctgcc gttccttatc gccaacacca aacagttaat gggctttggt
    38161 gaaacccgcg acgaaccgtt cgtcagcatg gaaggcaaac gcgtggtggt ccttggcggt
    38221 ggcgacactg cgatggactg cgtgcgcacg tctgtacgcc agggggcaaa gcacgttacc
    38281 tgtgcctatc gtcgtgatga agagaacatg ccgggttccc gccgcgaagt gaaaaacgcg
    38341 cgggaagaag gcgtagagtt caaattcaac gtccagccgc tgggtataga agtgaatggc
    38401 aacggcaaag tcagcggcgt aaaaatggtg cgcacagaaa tgggcgaacc ggatgccaaa
    38461 ggccgtcgcc gcgcggagat tgtggcaggt tccgaacata tcgttccggc agatgcggtg
    38521 atcatggcgt ttggtttccg tccacacaac atggaatggc tggcaaaaca cagcgtcgag
    38581 ctggattcgc aggggcgcat tatcgccccg gaagacaacg acaacgcttt ccagaccagc
    38641 aacccgaaaa tctttgctgg cggcgatatc gtccgtggtt ccgatctggt ggtgaccgct
    38701 attgccgaag gtcgtaaggc ggcagacggt attatgaact ggctggaagt ttaagcctgt
    38761 cccagcgccg cctcctcaga aaagagtcgg cgctgaaatt ctcctttctt tctggaaagc
    38821 gcctcgcata atctgaagaa aaccaatgta atacgctcat ctggattgtt accggtagat
    38881 gcccggcgta tagctgattc cattattcaa cgcagcgcca tataaaggga cagataatgg
    38941 aatcgctctc agagagaacc tcaacaggct accagcaaat ccacgacggt attattcacc
    39001 tggtcgatag cgcccggacg gaaacggtac gtagcgttaa cgcgctgatg accgcgacat
    39061 actgggaaat tggccgacga attgtcgaat ttgaacaagg tggcgaggcc agggctgcgt
    39121 atggtgcgca gctaatcaag cgactatcaa aggatttaag tctaaggtat aagcgtgggt
    39181 tctctgcaaa aaacttacgc caaatgaggc ttttttacct cttttttcaa catgttgaaa
    39241 ttcgccagac agtgtctggc gaattaacac cattgggaat tccgcagaca ccatctgcag
    39301 aattcccatc ggcaaagatt tggcagacgc tgtctgccaa atctttcccc ctgccctggt
    39361 ccacctacgt ccgtttactt tccgttaaaa accctgatgc ccgcagcttt tatgaaaaag
    39421 agacgctccg ctgtggttgg tctgtccgcc agctagagcg gcaaattacg acccaatttt
    39481 atgagcgaac actactgtca catgacaaat cagccatgct gcaacaacac gctcctgccg
    39541 agatgcatat tcttccgcaa caggcgatac gcgatccctt tgtgctcgaa tttctggagt
    39601 tgaaagatga atactcagaa tctgattttg aggaggcgct gatcaaccac ctgatggatt
    39661 tcatgctgga acttggggat gattttgcct ttgttggtcg gcagcgaagg ttacgcattg
    39721 atgacaactg gtttcgggtc gatctgctgt ttttccaccg ccgtttacgc tgcctgctaa
    39781 tcgtcgatct aaaagtgggc aaattcagct atagcgatgc cggacagatg aatatgtatc
    39841 tcaactacgc caaagagcac tggacactac cggatgaaaa tccgcccatc ggtctggttc
    39901 tctgtgcaga gaaaggagtc ggcgaagcgc attatgcact ggctggtttg cctaacaccg
    39961 ttctggcgag tgaatataag atgcaactac ctgatgaaaa acgactcgcg gatgaactcg
    40021 ttcgaacaca ggcggtgtta gaggaaggct atagacgccg ttaatttcag cggtttcgaa
    40081 ttctccagac actgtctgga gaatctttaa aaagaactaa gttttaagcc attaaatcag
    40141 ccttaacctt caccacaacc tttttaatct caccaggttc gccgacaacg catcctggtt
    40201 tatgcggttc tcccggcata aacacggcga acattcccgg ttttaagatg atggcttgct
    40261 cgttctcaat ggcgctgcaa agctggtaat catcctcatg gtggaactct tcacactgac
    40321 gcgcagtgcc tgccgtgcca aacagaatcc gttcctcacc gtttaataac agctggatat
    40381 caatgtattg ctcgtgcagc tccgcttttt tctcaacggg cgattgagtg ttaaacgtca
    40441 tgacattcat aaagatattg tcgcactgta attcgtaacg acccggtgct ttttcttgcg
    40501 gcctggcggc taaggctagt gttaacgcgt cctgtaacgc aggatgcaat ccggcagacg
    40561 gtaacgactg tacttcaccc attatcataa tttttctccc tgggccaaca acgcagcccc
    40621 aagtaaacct gcatcgtggc ggtagtgcgc cgccagtaaa tcaacatgaa atgccgctgg
    40681 ctcctgcgcc agatacgttt ccaccaacgc cagataccct tctgccagac caacgctgcc
    40741 accgaccact acgcactggc aatcagttgt ggctttaata tcagcgatca gccttgccag
    40801 cacatgtgcg gagcagtgaa tcagttgctg cgcctgctca tcgccctgcc cggcgcgtgt
    40861 gaaaatagtt ctcgcatccg cgcccgccag ttcaccctgc gctgccgctg caataccgcg
    40921 accagaagca atcgcttcca cacaaccagt gcgtccacag ccgcagactg gaccatgagg
    40981 atcagccagc gtatgcccga tgtgccccgc cagaccgcca gggccggtga gcagtttgcc
    41041 gccgctcact acaccgccgc caacaccagt ggaaacggtg ataaacacca tctcggttat
    41101 atcgccttcc agcgcctgat actccgccca cgctgcggcc tgcgcgtcgt taatggcaat
    41161 ggtcggcaaa tcggtaagtt gttccaaagt tttgactaac ggaaagtgta gcaatccacc
    41221 gagattatgc ggattcagcg ccagcaggct gccgtcgcga ataattccgg ttgaagcgat
    41281 agcaacccgc tgcgcatgaa cttgcaacgg agagactaat gcggataagg catcatgcaa
    41341 ggcttctggt gtctggctgg ctggcgtagg aagttcacga cgatcgcgga tctgcccatc
    41401 agcgccaatc agcgcggcgg caagtttagt accgccgata tcaatcgcca gtgtggtcat
    41461 agcaccgcct ttttcatcgc tgtgttgtac cactgacaaa tgtgttcaag acgcgtaatg
    41521 gcagaaccga ccgtcaccgc ccacgcgccg tggcgcatcg catctgccgc ctgagcaggc
    41581 gtgttgtaac gcccttcggc aatcacccga catccggcgt cgctcaacgt tttcaccagc
    41641 gccagatccg gctcttctgg cgtttcaggc gtggtatagc cagaaagcgt agtgccaata
    41701 atttcggctc ccagcttttg gcatgccagg ccgtcttccg gcgttgagca gtcggtcatc
    41761 gccagtaaac cgtgatggtg aatacgtgcc agcagcgttt caacaggcac cggacgcggt
    41821 cggtcggtgc cgtcaatggc gataatgtcc gcgcccgcct gcgccagcgc atcaacatct
    41881 tcaatatagg ccgtgatgcg taccggagaa tcctccagat cgcgtttcac aatcccgata
    41941 attggcacgc tcaccaccgc acgcgtggct tgcagatttg ccacaccttc aatgcgaatg
    42001 gcaaccgcgc ccgcctgttc tgccgctaat gccatggcgg cgacgatttc gggtttatcg
    42061 agcgggctgt ccggaaccgg ctggcaggag acaatcaggc caccgttagc agcgattttt
    42121 tgatccagtt gtgcaagtaa cgacatacat cttcccttag cgaaaggccc ggtacagaga
    42181 ccgggcaaca ggattaactt ttggttttga ctaaatcgtt tttggcgcgg ccaaatggca
    42241 cggcaccgct aaatggtttg ccgtcgatag cgtcatgagt acgcaatgct tccgggcgca
    42301 gccaacgctg aacgcgggaa ggcatatcca gaccaatcag caggatcacc acaaatgtca
    42361 gactgaacga gagcgatgcc agcgcagtac ccagatccag acgttgagcg atcaacgcgc
    42421 cgatgattgg ggccagtgca ccgcccaatg cgccgacgtt gtaggtaaag cccaggcccg
    42481 ctgcacgctg gtcggtatcg aaataaccgc caatcagttt cggtaagatc ccggcgatcc
    42541 cttgtccaag catttgctgg aagaacagta acagaccgag cacccagacg tttgcgccgc
    42601 caatcgcaaa taccggaata atcagcagct gcgaggccag cagactacaa acgtacgctt
    42661 tgcgggttcc cagccagtca ccgaggaagc caccgacgca gcaccccacc gccgcgccaa
    42721 agccactaaa gaacagcaca ttggcgacag tatgcgggtc ataagccaga tcggttttca
    42781 gatacgttgg cagcagcgcc tgaatcggcc atgagtagag gaaagcaaac aacacgacca
    42841 ccatcagcat tacgcccgtt ggccagcgtt tgcccgtact ctgcaccata aagctgataa
    42901 agattgcggc gcataacagc ccgagtacag cgacgattgc ggcattttgc aggttaccgg
    42961 cgaagcagaa ccacagcgca gtagccgccg ccagcgtcat tacgatattg gcaatgcgat
    43021 gttcaccacg gtaaagaatg tctaccatcg tgcgtactgg tgctttaccc ccgtgtttct
    43081 ctttccagtc ttccgcttcc gggatgtttt tacgcagcca gagagcaaag atgattggca
    43141 aaatgccgat aaagaacagc gctctccagc cccagaccgg aaccaccaga ctatagacct
    43201 gagcggcaac gacggccccc acagagaagc ctgaaatcaa aaaaccactg gctttgttac
    43261 gcagatgttt tggccagctt tcaatgacat aggtggcgct ggaaccgtat tcacccgcca
    43321 tccccatgcc gatgaccaga cgcgcgataa acatggtgat gtagcctggc gcaaagccgc
    43381 aggccagcgt cccggccgag aagagaacga tgctggtgac cattgccaga cgacgcccgt
    43441 agcggtcacc catagcgccg agcatcaggc cgccgaacca gcgagagata aaggctgcgg
    43501 agatcagact tgccgcctgc accgtcgtca gcccgaattc accctgtact tcggtgagta
    43561 ccagggcgat taaaacgaaa tcaaaaccgt caagcagata tcccaaccag gcagcggaaa
    43621 atgcgcgcca ttgtgcacgg ttgagatggc gataccacgg gatattctgg gttgtagtac
    43681 tcattgtgag tctcccgcgg tgggcgatgc ccacacgctt tggtatgaaa attgtagggt
    43741 acagatgcgt ttatttcccc tcacccggta ggggcgagcg aggggaaaac aactcacccg
    43801 cgctcttgca tcaactgctg ggccagcgcc ttcagttctg gcagatattt ttcatctacc
    43861 ggtccaaacg gtttgcggca cagcggcaca gaaacgacat ccatataatg caggacagtt
    43921 ttcaggccgc ggaatacgcc cgttttgatc agtaaatcaa tgactttgtt gcactcagtt
    43981 tgcagtttct gcgcggtctg gatatcgcct tctttcagcg ccttcacgat cccctgatat
    44041 cgccagccca tgatgttgta agtactgccg ataccaccat cagcgcctgc cagcaagccg
    44101 gaagcgaaga tttcgtcgta accgttatag agcaccagat caggatgttc acgacggatc
    44161 tgttccatct gatagagatc gccagaagtc tgtttcagcg caccgacgcc aggcaacgta
    44221 accagggtgt tgatctgatc cagggtcagt tttaccccac tcagggctgg aatgttgtac
    44281 accaccatcg gcaaaccatc cgccgaatca ataattgccc gatagtgatc gcagtgttct
    44341 tcaaagctga aaggatagta gaacggcgtg acggcggaga cggcatcgaa gccataacgt
    44401 ttagccgatg ccgcaagttg ttggctttcg gcggtgctga cgcaaccgac gtgggcgatg
    44461 agtttaatct tacctttcgc ctcttcagcg acgatttcca gtacctgttc acgctcggaa
    44521 aggctttgta caaaggcttc gccggtagaa ccacccacgt ataaaccgtc gatgccctgc
    44581 tgaatattga actgaaccag gcgacgcaga ctcgctttat ccagtgcttg ttgttgatca
    44641 aaaggagtca ggagtgcggc cattacgcca cgtaaattcg ttgccataaa tacctctgaa
    44701 gtgatgctta tctgataaac gatatacctt tatacctgtt ataccagatc aattaagcaa
    44761 caccccatac agaaagctta taatgcgatc tgcttcacta aagtggcatt atttcttttt
    44821 gttggtggtc tgaccgaagg cgtgccaggt agcagagacg ctgttgagat gcgattgcaa
    44881 cgcacgatcg gcttcatcgg gatcgtggcg gcggatcgcg tcaacgatcg caatatgctg
    44941 ttgatagcta acgttgttat gctcgtgcag agcctgatca gccacggttg gacgtgcggc
    45001 gatcagccag tcgagcaagg caacgtggat cgccatgaag attgggttac cggggatctc
    45061 cgccagcacg cggtggaaat caacgtctga acgaatgaat gccgcgttgt tatccagcga
    45121 ctgactgttg atttccagtg cttttgccag caaatcgatt tgctcatcgg tggcgtgttc
    45181 agccgcatag cgcaccaggc tggattcaaa gaacagacgt aattgttcga aatgggcaat
    45241 cccgccagga tgagaaagga aatctttcgc catgccggaa agctcgccga tgatggtgtc
    45301 cgcagaagga cgtgagacgc gagcgcgttc gccgttgttt atctgcacca gacctttgcg
    45361 ttttaacgct gccagcgctt cgcgcaccga aggacgcccg acgttaaaga acgccatcag
    45421 ttcgcgttca gacggtaatt gttcaccttc gccaaattca cgacggcgga tcatctgttc
    45481 cagctcttct tccaccattt cggagagttt tttacgcgcc agcgggcggc tgcgtaagtt
    45541 gcggccaatt gcaggtgaag aatcttcggt ttgcgaatca aatgcgttca taaggcccat
    45601 tctgtaaggt cagtgtgatt aacatcatca gtgacatcct atcacaggat tgaaagtagg
    45661 gggaaatggc ggggttttct ctttgtgc
//
LOCUS       DAZFLD010000011        42195 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.011, whole genome shotgun sequence.
ACCESSION   DAZFLD010000011 DAZFLD010000000
VERSION     DAZFLD010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 42195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42195
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.011"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            91..981
                     /gene="alsR"
                     /locus_tag="ACHKBL_000558"
     CDS             91..981
                     /gene="alsR"
                     /locus_tag="ACHKBL_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-allose utilization transcriptional regulator
                     AlsR"
                     /protein_id="HFO5817480.1"
                     /translation="MSQSEFDSALPNGIGLAPYLRMKQEGMTENESRIVEWLLKPGNL
                     SCAPAIKDVAEALAVSEAMIVKVSKLLGFSGFRNLRSALEDYFSQSEQVLPSELAFDE
                     APQDVVNKVFNITLRTIMEGQSIVNVDEIHRAARFFYQARQRDLYGAGGSNAICADVQ
                     HKFLRIGVRCQAYPDAHIMMMSASLLQEGDVVLVVTHSGRTSDVKAAVELAKKNGAKI
                     ICITHSYHSPIAKLADYIICSPAPETPLLGRNASARILQLTLLDAFFVSVAQLNIEQA
                     NINMQKTGAIVDFFSPGALK"
     gene            1040..1975
                     /gene="alsB"
                     /locus_tag="ACHKBL_000559"
     CDS             1040..1975
                     /gene="alsB"
                     /locus_tag="ACHKBL_000559"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-allose transporter substrate-binding protein"
                     /protein_id="HFO5817481.1"
                     /translation="MNKYLKYFSGTLVGLMLSTSAFAAAEYAVVLKTLSNPFWVDMKK
                     GIEDEAKTLGVSVDIFASPSEGDFQSQLQLFEDLSNKNYKGIAFAPLSSVNLVMPVAR
                     AWKKGIYLVNLDEKIDMDNLKKAGGNVEGFVTTDNVAVGAKGASFIIDKLGAEGGEVA
                     IIEGKAGNASGETRRNGATEAFKKASQIKLVASQPADWDRIKALDVATNVLQRNPNIK
                     AIYCANDTMAMGVAQAVANAGKTGKVLVVGTDGIPEARKMVEAGQMTATVAQNPADIG
                     ATGLKLMVDAEKSGKVIPLDKAPEFKLVDSILVTQ"
     gene            2103..3635
                     /gene="alsA"
                     /locus_tag="ACHKBL_000560"
     CDS             2103..3635
                     /gene="alsA"
                     /locus_tag="ACHKBL_000560"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-allose ABC transporter ATP-binding protein
                     AlsA"
                     /protein_id="HFO5817482.1"
                     /translation="MATPYISMAGIGKSFGPVHALKSVNLTVYPGEIHALLGENGAGK
                     STLMKVLSGIHEPTKGTITINNINYNKLDHKLAAQLGIGIIYQELSVIDELTVLENLY
                     IGRHLTKKICGVNIIDWREMRVRAAMMLLRVGLKVDLDEKVANLSISHKQMLEIAKTL
                     MLDAKVIIMDEPTSSLTNKEVDYLFLIMNQLRKEGTAIVYISHKLAEIRRICDRYTVM
                     KDGSSVCSGMVSDVSNDDIVRLMVGRELQNRFNAMKENVSNLAHDTVFEVRNVTSRDR
                     KKVRDISFSVCRGEILGFAGLVGSGRTELMNCLFGVDKRAGGEIRLNGKDISPRSPLD
                     AVKKGMAYITESRRDNGFFPNFSIAQNIAISRSLKDGGYKGAMGLFHEVDEQRTAEAQ
                     RELLALKCHSVNQNITELSGGNQQKVLISKWLCCHPEVIIFDEPTRGIDVGAKAEIYK
                     VMRQLADDGKVILMVSSELPEIITVCDRIAVFCEGRLTQILTNRDDMSEEEIMAWALP
                     HE"
     gene            3614..4594
                     /gene="alsC"
                     /locus_tag="ACHKBL_000561"
     CDS             3614..4594
                     /gene="alsC"
                     /locus_tag="ACHKBL_000561"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-allose ABC transporter permease"
                     /protein_id="HFO5817483.1"
                     /translation="MGFTTRVKSEASEKKPFNFALFWDKYGTFFILAIIVAIFGSLSP
                     EYFLTTNNITQIFVQSSVTVLIGMGEFFAILVAGIDLSVGAILALSGMVTAKLMLAGI
                     DPFLAALIGGVLVGGALGAINGCLVNWTGLHPFIITLGTNAIFRGITLVISDANSVYG
                     FSFDFVNFFAASVIGIPVPVIFSLIVALILWFLTTRMRLGRNIYALGGNKNSAFYSGI
                     DVKFHILVVFIISGVCAGLAGVVSTARLGAAEPLAGMGFETYAIASAIIGGTSFFGGK
                     GRIFSVVIGGLIIGTINNGLNILQVQTYYQLVVMGGLIIAAVALDRLISK"
     gene            4605..5300
                     /gene="alsE"
                     /locus_tag="ACHKBL_000562"
     CDS             4605..5300
                     /gene="alsE"
                     /locus_tag="ACHKBL_000562"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-allulose-6-phosphate 3-epimerase"
                     /protein_id="HFO5817484.1"
                     /translation="MKISPSLMCMDLLKFKEQIEFIDNHADYFHIDIMDGHFVPNLTL
                     SPFFVSQVKKLASKPLDCHLMVTRPQDYIAQLARAGADFITLHPETINGQAFRLIDEI
                     RRHGMKVGLILNPETPVEAMKYYIHKADKITVMTVDPGFAGQPFIPEMLDKLAELKAW
                     REREGLEYEIEVDGSCNQATYEKLMAAGADVFIVGTSGLFNHAENIDEAWRIMTAQIL
                     AAKSEVQPHAKTA"
     gene            5284..6213
                     /gene="alsK"
                     /locus_tag="ACHKBL_000563"
     CDS             5284..6213
                     /gene="alsK"
                     /locus_tag="ACHKBL_000563"
                     /EC_number="2.7.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0019316 - D-allose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allose kinase"
                     /protein_id="HFO5817485.1"
                     /translation="MQKQHNVVAGVDMGATHIRFCLRTAEGETLHCEKKRTAEVIAPD
                     LVSGIGEMIDEQLRRFNARCHGLVMGFPALVSKDKRTIISTPNLPLTAADLYDLADKL
                     ENTLNCPVEFSRDVNLQLSWDVVENRLTQQLVLAAYLGTGMGFAVWMNGAPWTGAHGV
                     AGELGHIPLGDMTQHCACGNSGCLETNCSGMALRRWYEQQPRNYPLSDLFVHAENAPF
                     VQSLLENAARAIATSINLFDPDAVILGGGVMDMPAFPRETLIAMTQKYLRRPLPYQVV
                     RFIAASSSDFNGAQGAAILAHQRFLPQSCAKVP"
     gene            6486..8471
                     /gene="yjcS"
                     /locus_tag="ACHKBL_000564"
     CDS             6486..8471
                     /gene="yjcS"
                     /locus_tag="ACHKBL_000564"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl sulfatase YjcS"
                     /protein_id="HFO5817486.1"
                     /translation="MNNSRLFRLSRIVIALTAASSMMVNTANATDEAKAATQYTQQVN
                     QNYAKSLPFSDRQDFDDAQRGFIAPLLDEGILRDANGKIYYRANDYKFDINAAAPETI
                     NPSLWRQSQINGISGLFKVTDKMYQVRGQDISNITFVEGEKGIIVIDPLVTPPAAKAA
                     LDLYFQHRPQKPIVAVIYTHSHADHYGGVKGIISEADVKSGKVQVIAPAGFMDEAISE
                     NVLAGNIMSRRALYSYGLLLPHNAQGNVGNGLGVTLATGDPSIIAPTKTIVRTGEKMI
                     IDGLEFDFLMTPGSEAPAEMHFYIPALKALCTAENATHTLHNFYTLRGAKTRDTSKWT
                     EYLNETLDMWGNDAEVLFMPHTWPVWGNKHINDYIGKYRDTIKYIHDQTLHLANQGYT
                     MNEIGDMIKLPPALANNWASRGYYGSVSHNARAVYNFYLGYYDGNPANLHPYGQVEMG
                     KRYVQALGGSARVINLAKEANKQGDYRWSAELLKQVIAANPGDQVAKNLQADNFEQLG
                     YQAESATWRGFYLTGAKELREGVHKFSHGTTGSPDTIRGMSVEMLFDFMSVRLDSAKA
                     AGKNISLNFNMSNGDNLNLTLNDSVLNYRKTLQPEANASFYISREDLHAVLTGQVKMV
                     DLVKAKKAKIIGNGAKLEEIIACLDNFDLWVNIVTPN"
     gene            8680..8955
                     /gene="ytcA"
                     /locus_tag="ACHKBL_000565"
     CDS             8680..8955
                     /gene="ytcA"
                     /locus_tag="ACHKBL_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtcA family lipoprotein"
                     /protein_id="HFO5817487.1"
                     /translation="MPTVLSRMAMQLKKTAWIIPVFMVSGCSLSPAIPVIGAYYPGWF
                     FCAIASLILTLITRRIIQRANINLAFVGIIYTALFALYAMLFWLAFF"
     gene            8974..10005
                     /gene="mdtN"
                     /locus_tag="ACHKBL_000566"
     CDS             8974..10005
                     /gene="mdtN"
                     /locus_tag="ACHKBL_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter periplasmic adaptor
                     subunit MdtN"
                     /protein_id="HFO5817488.1"
                     /translation="MESTPKKAPRSKFPALLVVALALVALVFVIWRVDSAPSTNDAYA
                     SADTIDVVPEVSGRIVELAVTDNQAVKQGDLLFRIDPRPYEANLAKAEASLAALDKQI
                     MLTQRSVDAQQFGADSVNATVEKARAAAKQATDTLRRTEPLLKEGFVSAEDVDRARTA
                     QRAAEADLNAVLLQAQSAASAVSGIDALVAQRAAVEADIALTKLHLEMATVRAPFDGR
                     VISLKTSVGQFASAMRPIFTLIDTRHWYVIANFRETDLKNIRSGTPATIRLMSDSGKT
                     FEGKVDSIGYGVLPDDGGLVLGGLPKVSRSINWVRVAQRFPVKIMVDKPDPEMFRIGA
                     SAVANLEPQ"
     gene            10005..12056
                     /gene="mdtO"
                     /locus_tag="ACHKBL_000567"
     CDS             10005..12056
                     /gene="mdtO"
                     /locus_tag="ACHKBL_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313090.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter permease subunit
                     MdtO"
                     /protein_id="HFO5817489.1"
                     /translation="MSALNSLPLPVVRLLAFFHEELSERRPGRVPQTVQLWVGCLLVI
                     LISMTFEIPFVALSLAVLFYGIQSNAFYTKFVAILFVVATVLEIGSLFLIYKWSYGEP
                     LIRLIIAGPILMGCMFLMRTHRLGLVFFAVAIVAIYGQTFPAMLDYPEVVVRLTLWCI
                     VVGLYPTLLMTLIGVLWFPSRAITQMHQALNDRLDDAISHLTDSLAPLPETRIEREAL
                     ALQKLNVFCLADDANWRTQSAWWQSCVATVTYIYSTLNRYDPTSFADSQAIIEFRQKL
                     ASEINKLQHAIAEGQCWQSDWRITESEAMAARECNLENICQTLLQLGQMDPNTPPTPA
                     TKPPSMVADAFTNPDYMRYAVKTLLACLICYTFYSGVDWEGIHTCMLTCVIVANPNVG
                     SSYQKMVLRFGGAFCGAILALLFTLLVMPWLDNIVELLFVLAPIFLLGAWIATSSERS
                     SYIGTQMVVTFALATLENVFGPVYDLVEIRDRALGIIIGTVVSAVIYTFVWPESEART
                     LPQKLAGALGMLSKVMRIPRQQEVTALRTYLQIRIGLHAAFNACEEMCQRVALERQLD
                     SEERALLIERSQTVIRQGRDILHAWDATWNSAQALDNALQPDRAGQFADALEKYAAGL
                     ATALSRSPQITLEETPASQAILPTLLKQEQHVCQLFARLPDWTAPALTPATEQVQGAT
                     Q"
     gene            12053..13519
                     /gene="mdtP"
                     /locus_tag="ACHKBL_000568"
     CDS             12053..13519
                     /gene="mdtP"
                     /locus_tag="ACHKBL_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MdtP"
                     /protein_id="HFO5817490.1"
                     /translation="MINRQLSRLLLCSILGSTTLISGCALVRKDSAPHQQLKPEQIKL
                     ADDIHLASSGWPQAQWWKQLNDPQLDALIQRTLSGSHTLAEAKLREEKAQSQADLLDA
                     GSQLQVAALGMLNRQRVSANGFLSPYAMDAPALGMDGPFYTEATVGLFAGLDLDLWGV
                     HRSAVAAAIGAHNAALAETAAVELSLTTGVAQLYYSMQASYQMLDLLEQTRDVIDYAV
                     KAHQSKVAHGLEAQVPFHGARAQILAVDKQIVAVKGQITETRESLRALIGAGASDMPE
                     IKPVALPQVQTGIPATLSYELLARRPDLQAMRWYVQASLDQVDSARALFYPSFDIKAF
                     FGLDSIHLDTLFKKTSRQFNFIPGLKLPLFDGGRLNANLEGTRAASNMMIERYNQSVL
                     NAVRDVAVNGTRLQTLNDEREMQAERVEATRFTLRAAEAAYQRGLTSRLQATEARLPV
                     LAEEMSLLMLDSRRVIQSIQLMKSLGGGYQASPVVEKK"
     gene            13717..15864
                     /gene="fdhF"
                     /locus_tag="ACHKBL_000569"
     CDS             13717..15864
                     /gene="fdhF"
                     /locus_tag="ACHKBL_000569"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:14134..14136,aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HFO5817491.1"
                     /translation="MKKVVTVCPYCASGCKINLVVDNGKIVRAEAAQGKTNQGTLCLK
                     GYYGWDFINDTQILTPRLKTPMIRRQRGGKLEPVSWDEALNYVAERLSAIKEKYGPDA
                     IQTTGSSRGTGNETNYVMQKFARAVIGTNNVDCCARVUHGPSVAGLHQSVGNGAMSNA
                     INEIDNTDLVFVFGYNPADSHPIVANHVINAKRNGAKIIVCDPRKIETARIADMHIAL
                     KNGSNIALLNAMGHVIIEENLYDKAFVASRTEGFEEYRKIVEGYTPESVEDITGVSAS
                     EIRQAARMYAQAKSAAILWGMGVTQFYQGVETVRSLTSLAMLTGNLGKPHAGVNPVRG
                     QNNVQGACDMGALPDTYPGYQYVKDPANREKFAKAWGVESLPAHTGYRISELPHRAAH
                     GEVRAAYIMGEDPLQTDAELSAVRKAFEDLELVIVQDIFMTKTASAADVILPSTSWGE
                     HEGVFTAADRGFQRFFKAVEPKWDLKTDWQIISEIATRMGYPMHYNKTQEIWDELRHL
                     CPDFYGATYEKMGELGFIQWPCRDTSDTDQGTSYLFKEKFDTPNGLAQFFTCDWVAPI
                     DKLTDEYPMVLSTVREVGHYSCRSMTGNCAALAALADEPGYAQINTEDAKRLGIEDEA
                     LVWVHSRKGKIITRAQVSDRPNKGAIYMTYQWWIGACNELVTENLSPITKTPEYKYCA
                     VRVEPIADQRAAEQYVIDEYNKLKTRLREAALA"
     gene            15958..16647
                     /gene="yjcO"
                     /locus_tag="ACHKBL_000570"
     CDS             15958..16647
                     /gene="yjcO"
                     /locus_tag="ACHKBL_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sel1 family TPR-like repeat protein YjcO"
                     /protein_id="HFO5817492.1"
                     /translation="MKKIIALMLFLTFFAHANDSEPGSQYLKAAEAGDRRAQYFLADS
                     WFSSGDLSKAEYWAQKAADSGDADACALLAQIKITNPVSLDYPQAKVLAEKAAQAGSK
                     EGEVTLAHILVNTQAGKPDYPKAISLLENASEDLENDSAVDAQMLLGLIYANGVGIKA
                     DDDKATWYFKRSSAISRTGYSEYWAGMMFLNGEEGFIEKNKQKALHWLNLSCMEGFDT
                     GCEEFEKLTNG"
     gene            complement(17065..18378)
                     /gene="gltP"
                     /locus_tag="ACHKBL_000571"
     CDS             complement(17065..18378)
                     /gene="gltP"
                     /locus_tag="ACHKBL_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006835 - dicarboxylic acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate:proton symporter GltP"
                     /protein_id="HFO5817493.1"
                     /translation="MKNIKFSLAWQILFAMVLGILLGSYLHYHSDSRDWLVVNLLSPA
                     GDIFIHLIKMIVVPIVISTLVVGIAGVGDAKQLGRIGAKTIIYFEVITTVAIILGITL
                     ANVFQPGAGVDMSQLATVDISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEM
                     LPIIFFSVLFGLGLSSLPATHREPLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVT
                     VANFGFSSLWPLAKLVLLVHFAILFFALVVLGIVARLCGLSVWILIRILKDELILAYS
                     TASSESVLPRIIEKMEAYGAPASITSFVVPTGYSFNLDGSTLYQSIAAIFIAQLYGID
                     LSIWQEIILVLTLMVTSKGIAGVPGVSFVVLLATLGSVGIPLEGLAFIAGVDRILDMA
                     RTALNVVGNALAVLVIAKWEHKFDRKKALAYEREVLGKFDKTADQ"
     gene            complement(18720..19316)
                     /gene="nrfG"
                     /locus_tag="ACHKBL_000572"
     CDS             complement(18720..19316)
                     /gene="nrfG"
                     /locus_tag="ACHKBL_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfG"
                     /protein_id="HFO5817494.1"
                     /translation="MKQSQIPVKMLTTLTILMAFLCIGSYLLSPKWQAVRAEYQRQRD
                     PLHQFASQQTPEAQLQALQDKIRANPQNSEQWALLGEYYLWQNDYSNSLLAYRQALQL
                     RGENAELYAALATVLYYQASQHMTAQTRAMIDKALALDSNEITALMLLASDAFMQANY
                     AQAIELWQKVMDLNSPRINRTQLVESINMAKLLQRRSD"
     gene            complement(19313..19696)
                     /gene="nrfF"
                     /locus_tag="ACHKBL_000573"
     CDS             complement(19313..19696)
                     /gene="nrfF"
                     /locus_tag="ACHKBL_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HFO5817495.1"
                     /translation="MNKGLLTLLLLFTCFARAQVVDTWQFANPQQQQQALNIASQLRC
                     PQCQNQNLLESNAPVAVSMRHQVYSMVAEGKNEAEIIGWMTERYGDFVRYNPPLTGQT
                     LVLWALPVVLLLLMALILWRVRAKR"
     gene            complement(19689..21407)
                     /gene="nrfE"
                     /locus_tag="ACHKBL_000574"
     CDS             complement(19689..21407)
                     /gene="nrfE"
                     /locus_tag="ACHKBL_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HFO5817496.1"
                     /translation="MDVFLPEVGFLALLLSLGVNVLTPLTAFAGVRLRWPAMMRLTCI
                     GILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSYSLLSWELKLAAVWGGHEGSLLLW
                     VLLLSAWSALFAWHYRQQTDPLFPLTLAVLSLMLAALLLFVVLWSDPFVRIFPPAIEG
                     RDLNPMLQHPGLIFHPPLLYLGYGGLMVAASVALASLLRGEFDGACARICWRWALPGW
                     SALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATALLHSLSLTRQRGIFRH
                     WSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFALISLASLALYGW
                     RARDGGPAVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPY
                     FNRATLPFGLLMLVVIVLATFVSGKRVQLPALVAHAGVLLFAAGIVVSSVSRQEISLN
                     LQPGQQVTLAGYTFRFERLDLQARGNYTSEKAIVALFDHQQRIGELTPERRFYEARRQ
                     QMMEPSIRWNGIHDWYAVMGEKTGADRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWR
                     GRKRDE"
     gene            complement(21427..22383)
                     /gene="nrfD"
                     /locus_tag="ACHKBL_000575"
     CDS             complement(21427..22383)
                     /gene="nrfD"
                     /locus_tag="ACHKBL_000575"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HFO5817497.1"
                     /translation="MMQTSAFHFESLVWDWPIAIYLFLIGISAGLVTLAVLLRRFYPQ
                     AGGADSTLLRTTLIVGPGAVILGLLILVFHLTKPWTFWKLMFHYSFTSVMSMGVMLFQ
                     LYMVVLVLWLAKIFEHDLLALQQRWLPKLGIVQKVLSLLTPVHRGLETLMLVLAVLLG
                     AYTGFLLSALKSYPFLNNPILPVLFLFSGISSGAAVALIAMAIRQRKNPHSTEAHFVH
                     RMEIPVVWGEIFLLVAFFVGLALGDDGKVRALVAALGGGFWTWWFWLGVAGLGLIVPM
                     LLKPWVNRSSGIPTVLAACGASLVGVLMLRFFILYAGQLTVA"
     gene            complement(22380..23051)
                     /gene="nrfC"
                     /locus_tag="ACHKBL_000576"
     CDS             complement(22380..23051)
                     /gene="nrfC"
                     /locus_tag="ACHKBL_000576"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HFO5817498.1"
                     /translation="MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDES
                     LCIGCTACMDACREVNKVPEGVSRLTIIRSEPQGEFPDVKYRFFRKSCQHCDHAPCVD
                     VCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTNL
                     QAGKLPACVEACPTKALTFGNLDDPNSEISQLLRQKPTYRYKLALGTKPKLYRVPFKY
                     GEVSQ"
     gene            complement(23048..23614)
                     /gene="nrfB"
                     /locus_tag="ACHKBL_000577"
     CDS             complement(23048..23614)
                     /gene="nrfB"
                     /locus_tag="ACHKBL_000577"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HFO5817499.1"
                     /translation="MSVLRSLLTAGVLASGLLWSLNGITATPAAQASGDRYEVTQQRN
                     PDAACLDCHKPDTEGMHGKHASVINPNNKLPVTCTNCHGQPSPQHREGVKDVMRFNEP
                     MYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
                     KICVDCHSDQRTNPNFNPASVPLLKEQP"
     gene            complement(23659..25095)
                     /gene="nrfA"
                     /locus_tag="ACHKBL_000578"
     CDS             complement(23659..25095)
                     /gene="nrfA"
                     /locus_tag="ACHKBL_000578"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0042279 - nitrite reductase (cytochrome,
                     ammonia-forming) activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HFO5817500.1"
                     /translation="MTRIKINARRIFSLLIPFFFFTSVHAEQTAAPAKPVTVEAKNET
                     FAPQHPDQYLSWKATSEQSERVDALAEDPRLVILWAGYPFSRDYNKPRGHAFAVTDVR
                     ETLRTGAPKNAEDGPLPMACWSCKSPDVARLIQKDGEDGYFHGKWARGGPEIVNNLGC
                     ADCHNTASPEFAKGKPELTLSRPYAARAMEAIGKPFEKAGRFDQQSMVCGQCHVEYYF
                     DGKNKAVKFPWDDGMKVENMEQYYDKIAFSDWTNSLSKTPMLKAQHPEYETWTAGIHG
                     KNNVTCIDCHMPKVQNAEGKLYTDHKIGNPFDNFAQTCANCHTQDKAALQKVVAERKQ
                     SINDLKIKVEDQLVHAHFEAKAALDAGATEAEMKPIQDDIRHAQWRWDLAIASHGIHM
                     HAPEEGLRMLGTAMDKAADARTKLARLLATKGITHEIQIPDISTKEKAQQAIGLNMEQ
                     IKAEKQDFIKTVIPQWEEQARKNGLLSQ"
     gene            25488..27446
                     /gene="acs"
                     /locus_tag="ACHKBL_000579"
     CDS             25488..27446
                     /gene="acs"
                     /locus_tag="ACHKBL_000579"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HFO5817501.1"
                     /translation="MSQIHKHTIPANIADRCLINPQQYEAMYQQSINAPDTFWGEQGK
                     ILDWIKPYQKVKNTSFAPGNVSIKWYEDGTLNLAANCLDRHLQENGDRTAIIWEGDDA
                     SQSKHISYKELHRDVCRFANTLLELGIKKGDVVAIYMPMVPEAAVAMLACARIGAVHS
                     VIFGGFSPEAVAGRIIDSNSRLVITSDEGVRAGRSIPLKKNVDDALKNPNVTSIEHVV
                     VLKRTGGKIDWQEGRDLWWHDLVEQASDQHQAEEMNAEDPLFILYTSGSTGKPKGVLH
                     TTGGYLVYAALTFKYVFDYHPGDIYWCTADVGWVTGHSYLLYGPLACGATTLMFEGVP
                     NWPTPARMAQVVDKHQVNILYTAPTAIRALMAEGDKAIEGTDRSSLRILGSVGEPINP
                     EAWEWYWKKIGNEKCPVVDTWWQTETGGFMITPLPGATELKAGSATRPFFGVQPALVD
                     NEGNPLEGATEGSLVITDSWPGQARTLFGDHERFEQTYFSTFKNMYFSGDGARRDEDG
                     YYWITGRVDDVLNVSGHRLGTAEIESALVAHPKIAEAAVVGIPHNIKGQAIYAYVTLN
                     HGEEPSPELYAEVRNWVRKEIGPLATPDVLHWTDSLPKTRSGKIMRRILRKIAAGDTS
                     NLGDTSTLADPGVVEKLLEEKQAIAMPS"
     gene            27828..28142
                     /gene="yjcH"
                     /locus_tag="ACHKBL_000580"
     CDS             27828..28142
                     /gene="yjcH"
                     /locus_tag="ACHKBL_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HFO5817502.1"
                     /translation="MNGTIYQRIEDNAHFRELVEKRQRFATILSIIMLAVYIGFILLI
                     AFAPGWLGTPLNPNTSVTRGIPIGVGVIVISFVLTGIYIWRANGEFDRLNNEVLHEVQ
                     AS"
     gene            28139..29788
                     /gene="actP"
                     /locus_tag="ACHKBL_000581"
     CDS             28139..29788
                     /gene="actP"
                     /locus_tag="ACHKBL_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015123 - acetate transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015370 - solute:sodium symporter activity
                     [Evidence IEA];
                     GO_function: GO:0031402 - sodium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation/acetate symporter ActP"
                     /protein_id="HFO5817503.1"
                     /translation="MKRVLTALAATLPFAANAADAISGAVERQPTNWQAIIMFLIFVV
                     FTLGITYWASKRVRSRNDYYTAGGNITGFQNGLAIAGDYMSAASFLGISALVFTSGYD
                     GLIYSLGFLVGWPIILFLIAERLRNLGRYTFADVASYRLKQGPIRILSACGSLVVVAL
                     YLIAQMVGAGKLIELLFGLNYHIAVVLVGVLMMMYVLFGGMLATTWVQIIKAVLLLFG
                     ASFMAFMVMKHVGFSFNNLFSEAMAVHPKGVDIMKPGGLVKDPISALSLGLGLMFGTA
                     GLPHILMRFFTVSDAREARKSVFYATGFMGYFYILTFIIGFGAIMLVGANPEYKDAAG
                     HLIGGNNMAAVHLANAVGGNLFLGFISAVAFATILAVVAGLTLAGASAVSHDLYANVF
                     KKGATEREELRVSKITVLILGVIAIILGVLFENQNIAFMVGLAFAIAASCNFPIILLS
                     MYWSKLTTRGAMLGGWLGLITAVVLMILGPTIWVQILGHEKAIFPYEYPALFSISVAF
                     LGIWFFSATDNSAEGARERELFRAQFIRSQTGFGVEQGRAH"
     gene            complement(29871..31520)
                     /gene="yjcE"
                     /locus_tag="ACHKBL_000582"
     CDS             complement(29871..31520)
                     /gene="yjcE"
                     /locus_tag="ACHKBL_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HFO5817504.1"
                     /translation="MEIFFTILIMTLVVSLSGVVTRVMPFQIPLPLMQIAIGALLAWP
                     TFGLHVEFDPELFLVLFIPPLLFADGWKTPTREFLEHGREIFGLALALVVVTVVGIGF
                     LIYWVVPGIPLIPAFALAAVLSPTDAVALSGIVGEGRIPKKIMGILQGEALMNDASGL
                     VSLKFAVAVAMGTMIFTVGGATVEFMKVAIGGILAGFVVSWLYGRSLRFLSRWGGDEP
                     ATQIVLLFLLPFASYLIAEHIGVSGILAAVAAGMTITRSGVMRRAPLAMRLRANSTWA
                     MLEFVFNGMVFLLLGLQLPGILETSLMAAEIDPNVEIWMLFTDIILIYAALMLVRFGW
                     LWTMKKFSNRFLKKKPMEFGSWTTREILIASFAGVRGAITLAGVLSIPLLLPDGNVFP
                     ARYELVFLAAGVILFSLFVGVVMLPILLQHIEVADHSQQLKEERIARAATAEVAIVAI
                     QKMEERLAADTEENIDNQLLTEVSSRVIGNLRRRADGRNDVESSVQEENLERRFRLAA
                     LRSERAELYHLRATREISNETLQKLLHDLDLLEALLIEENQ"
     gene            complement(31671..33020)
                     /gene="ghxP"
                     /locus_tag="ACHKBL_000583"
                     /gene_synonym="yjcD"
     CDS             complement(31671..33020)
                     /gene="ghxP"
                     /locus_tag="ACHKBL_000583"
                     /gene_synonym="yjcD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxP"
                     /protein_id="HFO5817505.1"
                     /translation="MSTPSARTGGSLDAWFKISQRGSTVRQEVVAGLTTFLAMVYSVI
                     VVPGMLGKAGFPPAAVFVATCLVAGLGSIVMGLWANLPLAIGCAISLTAFTAFSLVLG
                     QHISVPVALGAVFLMGVLFTVISATGIRSWILRNLPHGVAHGTGIGIGLFLLLIAANG
                     VGLVIKNPLDGLPVALGDFTTFPVMMSLVGLAVIIGLEKLKVPGGILLTIIGISIVGL
                     IFDPNVHFSGVFAMPSLSDENGNSLIGSLDIMGALNPVVLPSVLALVMTAVFDATGTI
                     RAVAGQANLLDKDGQIIDGGKALTTDSMSSVFSGLVGAAPAAVYIESAAGTAAGGKTG
                     LTAITVGVLFLLILFLSPLSYLVPGYATAPALMYVGLLMLSNVAKIDFADFVDAMAGL
                     VTAVFIVLTCNIVTGIMIGFATLVIGRLVSGEWRKLNIGTVVIAVALVTFYAGGWAI"
     gene            complement(33566..34030)
                     /gene="soxR"
                     /locus_tag="ACHKBL_000584"
     CDS             complement(33566..34030)
                     /gene="soxR"
                     /locus_tag="ACHKBL_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HFO5817506.1"
                     /translation="MEKKLPRIKALLTPGEVAKRSGVAVSALHFYESKGLISSIRNSG
                     NQRRYKRDVLRYVAIIKIAQRIGIPLATIREAFGVLPEGHTLSAKEWKQLSSQWREEL
                     DRRIHTLVALRDELDGCIGCGCLSRSDCPLRNPGDRLGEEGTGARLLEDEQN"
     gene            34116..34439
                     /gene="soxS"
                     /locus_tag="ACHKBL_000585"
     CDS             34116..34439
                     /gene="soxS"
                     /locus_tag="ACHKBL_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide response transcriptional regulator
                     SoxS"
                     /protein_id="HFO5817507.1"
                     /translation="MSHQKIIQDLIAWIDEHIDQPLNIDVVAKKSGYSKWYLQRMFRT
                     VTHQTLGDYIRQRRLLLAAVELRTTERPIFDIAMDLGYVSQQTFSRVFRRQFDRTPSD
                     YRHRL"
     gene            complement(34442..36028)
                     /gene="pdeC"
                     /locus_tag="ACHKBL_000586"
     CDS             complement(34442..36028)
                     /gene="pdeC"
                     /locus_tag="ACHKBL_000586"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
                     /protein_id="HFO5817508.1"
                     /translation="MSHRARHQLLALPGIIFLVLFPIILSLWIAFLWAKSEVNSQLQT
                     FAQLALDKSELVIRQADLVSDAAERYQGQVCTPAHQKRMLNIIRGYLYINELIYARDN
                     HFLCSSLIASVNGYTIAPADYKREPNVSIYYYRDTPFFSGYKMTYMQRGNYVAVINPL
                     FWSEVMSDDPTLQWGVYDTVTKTFFSLSNEASAATFSPLIHLNDLTVQRNGYLYATVY
                     STKRPIAAIVATSYQRLITHFYNHLIFALPAGILGSLVLLLLWLRIRQNYLSPKRKLQ
                     RALEKHQLCLYYQPIIDIKTEKCIGAEALLRWPGEQGQVMNPAEFIPLAEKEGMIEQI
                     TDYVIDNVFRDLGVYLATHVDRYVSINLSASDFHTSRLIARTNQKTEQYAVRPQQIKF
                     EVTEHAFLDVDKMTPIILAFRQAGYEVAIDDFGIGYSNLHNLKSLNVDILKIDKSFVE
                     TLTTHKTSHLIAEHIIELAHSLGLKTIAEGVETEEQVNWLRKRGVRYCQGWFFAKAMP
                     PQVFMQWMEQLPARELTRGQ"
     gene            36458..36739
                     /gene="yjcB"
                     /locus_tag="ACHKBL_000587"
     CDS             36458..36739
                     /gene="yjcB"
                     /locus_tag="ACHKBL_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcB family protein"
                     /protein_id="HFO5817509.1"
                     /translation="MATLTTGVVLLRWQLLSAVMMFLASTLNIRFRRSDYVGLAVISS
                     GLGVVSACWFAMGLLGITMADITAIWHNIESVMIEEMNQTPPQWPMILT"
     gene            complement(36839..37375)
                     /gene="ssb1"
                     /locus_tag="ACHKBL_000588"
     CDS             complement(36839..37375)
                     /gene="ssb1"
                     /locus_tag="ACHKBL_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SSB1"
                     /protein_id="HFO5817510.1"
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDK
                     ATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEV
                     VVNVGGTMQMLGGRQGGGAPAGGNIGGGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSA
                     PAAPSNEPPMDFDDDIPF"
     gene            37630..40452
                     /gene="uvrA"
                     /locus_tag="ACHKBL_000589"
     CDS             37630..40452
                     /gene="uvrA"
                     /locus_tag="ACHKBL_000589"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HFO5817511.1"
                     /translation="MDKIEVRGARTHNLKNINLVIPRDKLIVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIHDYLRLLYARVGEPRCPDHDVPLAAQTVSQMVDNVLSQPEGKRLMLLAPIIKERKG
                     EHTKTLENLASQGYIRARIDGEVCDLSDPPKLELQKKHTIEVVVDRFKVRDDLTQRLA
                     ESFETALELSGGTAVVADMDDPKAEELLFSANFACPICGYSMRELEPRLFSFNNPAGA
                     CPTCDGLGVQQYFDPDRVIQNPELSLAGGAIRGWDRRNFYYFQMLKSLADHYKFDVEA
                     PWGSLSANVHKVVLYGSGKENIEFKYMNDRGDTSIRRHPFEGVLHNMERRYKETESSA
                     VREELAKFISNRPCASCEGTRLRREARHVYVENTPLPAISDMSIGHAMEFFNNLKLAG
                     QRAKIAEKILKEIGDRLKFLVNVGLNYLTLSRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYVLDEPSIGLHQRDNERLLGTLIHLRDLGNTVIVVEHDEDAIRAADHVIDIGPGAGV
                     HGGEVVAEGPLEAIMAVPESLTGQYMSGKRKIEVPKKRVPANPEKVLKLTGARGNNLK
                     DVTLTLPVGLFTCITGVSGSGKSTLINDTLFPIAQRQLNGATIAEPAPYRDIQGLEHF
                     DKVIDIDQSPIGRTPRSNPATYTGVFTPVRELFAGVPESRARGYTPGRFSFNVRGGRC
                     EACQGDGVIKVEMHFLPDIYVPCDQCKGKRYNRETLEIKYKGKTIHEVLDMTIEEARE
                     FFDAVPALARKLQTLMDVGLTYIRLGQSATTLSGGEAQRVKLARELSKRGTGQTLYIL
                     DEPTTGLHFADIQQLLDVLHKLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGE
                     ILVSGTPETVAECEASHTARFLKPML"
     gene            complement(40487..40843)
                     /gene="yjbR"
                     /locus_tag="ACHKBL_000590"
     CDS             complement(40487..40843)
                     /gene="yjbR"
                     /locus_tag="ACHKBL_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFO5817512.1"
                     /translation="MTISELLQYCMAKPGAEQSVHNDWKATQIKVEDVLFAMVKEVEN
                     RPAVSLKTSPELAELLRQQHSDVRPSRHLNKAHWSTVYLDGSLPDSQIYYLVDASYQQ
                     AVNLLPEEKRKLLVQL"
     gene            complement(40847..41263)
                     /gene="yjbQ"
                     /locus_tag="ACHKBL_000591"
     CDS             complement(40847..41263)
                     /gene="yjbQ"
                     /locus_tag="ACHKBL_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HFO5817513.1"
                     /translation="MWYQKTLTLSAKSRGFHLVTDEILNQLADMPRVNIGLLHLLLQH
                     TSASLTLNENCDPTVRHDMERFFLRAVPDNGNYEHDYEGADDMPSHIKSSMLGTSLVL
                     PVHKGRIQTGTWQGIWLGEHRIHGGSRRIIATLQGE"
     gene            complement(41374..42087)
                     /gene="aphA"
                     /locus_tag="ACHKBL_000592"
     CDS             complement(41374..42087)
                     /gene="aphA"
                     /locus_tag="ACHKBL_000592"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003993 - acid phosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HFO5817514.1"
                     /translation="MRKITQAISAVCLLFALNSSAVALASSPSPLNPGTNVARLAEQA
                     PIHWVSVAQIENSLAGRPPMAVGFDIDDTVLFSSPGFWRGKKTFSPESEDYLKNPVFW
                     EKMNNGWDEFSIPKEVARQLIDMHVRRGDAIFFVTGRSPTKTETVSKTLADNFHIPAT
                     NMNPVIFAGDKPGQNTKSQWLQDKNIRIFYGDSDNDITAARDVGARGIRILRASNSTY
                     KPLPQAGAFGEEVIVNSEY"
BASE COUNT        10435 a        11188 c        10888 g         9684 t
ORIGIN      
        1 aattttattt ttttgtgaag ttgccagcat cttttctgtt cttgctgtgg tgatatagtg
       61 gcgtcttcaa ttcaaggaca agagaacgtg atgagccagt cagagtttga ttcagcgctt
      121 ccgaacggta tcgggttagc gccttacctg cgaatgaagc aggaaggaat gacagaaaac
      181 gaaagccgca tcgtggagtg gttactcaaa cccggtaacc tgagttgtgc acccgcaatt
      241 aaagatgtcg cagaagctct ggcggtatct gaagcgatga tcgttaaggt atcaaagctg
      301 ctggggttta gcggctttcg taacttacgc agtgcgctgg aagattattt ttctcagtca
      361 gaacaagtat tgccttccga gttggctttt gatgaagcgc cgcaggatgt ggtgaataag
      421 gtatttaaca tcactttacg caccattatg gaaggtcagt cgatcgtcaa cgttgatgag
      481 atccaccgtg ccgcccgctt tttctatcag gccagacagc gggatttgta cggtgccgga
      541 ggatcaaatg caatctgtgc tgatgtacag cacaagtttt tgcgcattgg cgtacgctgc
      601 caggcctatc cggacgctca catcatgatg atgtctgctt cgttgttaca ggaaggagat
      661 gtggtgctgg tggtgaccca ttccgggcga accagtgatg taaaagcggc tgtagaactg
      721 gcaaaaaaga acggggcaaa gattatttgt ataacgcata gctaccattc accgatagcg
      781 aagctggccg attatattat ttgctcaccg gccccggaaa caccgttatt aggtcgtaat
      841 gcctcggcaa gaatattaca actaactttg ctggacgcgt tttttgtctc tgtcgcccag
      901 ctcaacattg aacaagctaa tattaatatg caaaaaaccg gcgcaattgt tgatttcttc
      961 tcaccaggcg cgctgaaata agaaaaaggt aattccgcca tcgcggagtt acctatcccc
     1021 ttaccgtgag aacatgatta tgaataaata tctgaaatat ttcagcggca cactcgtggg
     1081 cttaatgttg tcaaccagcg cttttgctgc cgccgaatat gccgtcgtat tgaaaacact
     1141 ctccaatcca ttttgggtag atatgaaaaa aggcattgaa gatgaagcaa aaacgctggg
     1201 cgtcagcgtt gatatttttg cctctccttc agaaggcgat tttcaatctc aattgcagtt
     1261 atttgaagat ctcagtaata aaaattacaa aggtatcgcc ttcgcaccat tatcctcagt
     1321 gaacctggtc atgcctgtcg cccgcgcatg gaaaaaaggc atttatctgg tcaatctcga
     1381 tgaaaaaatc gacatggata atctgaaaaa agcaggcggt aatgtggaag gttttgtcac
     1441 caccgataat gttgccgtcg gggcgaaagg cgcgtcattc attattgaca agctgggtgc
     1501 cgaagggggt gaagtcgcaa tcattgaggg taaagccggt aacgcctccg gtgaaacgcg
     1561 tcgtaatggt gccaccgaag ccttcaaaaa agcaagccag atcaagcttg tcgccagcca
     1621 gcctgccgac tgggaccgta ttaaagcact ggatgtcgcc actaacgtgt tgcaacgtaa
     1681 tccgaatatt aaagcgatct attgcgcgaa tgacacgatg gcaatgggtg ttgctcaggc
     1741 tgtcgcaaac gccggaaaaa cgggaaaagt gctggtcgtc ggtacagatg gcattccgga
     1801 agcccgcaaa atggtggaag ccggacaaat gaccgcgacg gttgcccaga acccggcgga
     1861 tatcggcgca acgggtctga agctgatggt tgacgctgaa aaatccggca aggttatccc
     1921 gctggataaa gcaccggaat ttaaactggt cgattcaatc ctggtcactc aataaaacta
     1981 aatgatgccg gatggtgatg ctaatgaagt gtcttatccg gccaacaaaa taacctgaac
     2041 gtagacctga taagtgtagc ccatcaggca ataatacgat atttcgtgca gagaggttat
     2101 ttatggccac gccatatata tcgatggcgg ggatcggcaa gtcctttggt ccggttcacg
     2161 cattaaagtc ggttaattta acggtttatc ctggtgaaat acatgcatta ctaggagaaa
     2221 atggcgcggg taaatccacg ctaatgaaag ttttatccgg aatacatgag ccgaccaaag
     2281 gcaccattac cattaataac attaactata acaaactgga tcataaatta gcggcacaac
     2341 tcggcatcgg gattatttat caagaactca gcgttattga tgaattaacc gtactggaaa
     2401 atttatatat tggtcgtcat ctgacgaaaa aaatctgtgg cgtcaatatt atcgactggc
     2461 gagaaatgcg tgtccgcgcc gccatgatgt tattacgcgt gggcttgaaa gttgatttag
     2521 atgagaaagt ggcgaattta tctatcagcc acaagcagat gctggaaatt gccaaaacgc
     2581 tgatgctcga cgccaaagtc atcatcatgg atgaacccac ctcctcactc actaataaag
     2641 aggtggacta tttgtttctg atcatgaatc agttgcgtaa ggagggtacg gccatcgtct
     2701 atatctcgca taaattggcg gaaattcgcc gtatttgcga ccgctatacg gtgatgaaag
     2761 acggcagcag cgtttgcagc ggcatggtga gcgatgtgtc aaatgacgat atcgtccgtc
     2821 tgatggtagg tcgcgaactg caaaaccgtt ttaacgcgat gaaggagaat gtcagcaacc
     2881 ttgcgcacga tacggttttt gaggtgcgca acgtcaccag ccgtgacaga aaaaaggtcc
     2941 gggatatctc atttagcgtc tgccggggag aaatattagg ctttgccgga ctggtcggtt
     3001 ccggacgtac tgaactgatg aactgcctgt ttggcgttga taaacgcgct ggcggagaaa
     3061 tccgtcttaa tggcaaagat atctctccac gttctcccct ggatgccgtg aaaaaaggga
     3121 tggcttacat cactgaaagc cgccgggata acggcttttt ccccaacttt tctatcgctc
     3181 agaacatagc tatcagccgc agtctgaaag acggcggcta taaaggcgcg atggggttgt
     3241 ttcatgaagt tgacgagcaa cgtaccgctg aagcccagcg cgaactgctg gcgctgaaat
     3301 gtcattcggt aaaccagaac atcactgaac tctccggcgg taaccagcag aaagtcctga
     3361 tctccaaatg gctgtgctgt cacccggaag tgatcatttt cgatgaacct acccgtggca
     3421 tcgacgttgg cgcgaaagcc gaaatttaca aagtgatgcg ccaactggcg gacgacggaa
     3481 aagtcatcct gatggtgtca tctgaactac ctgaaattat caccgtctgc gaccgcatcg
     3541 ccgtgttctg cgaaggacga ctgacgcaaa tcctgaccaa tcgcgatgac atgagcgaag
     3601 aggagattat ggcatgggct ttaccacacg agtaaaaagc gaagcgagcg agaagaaacc
     3661 gttcaacttt gcgctgttct gggataaata cggtactttt tttatcctgg cgatcatcgt
     3721 cgccattttt ggttcgctgt cgccagaata ttttctgacc accaataata ttacccagat
     3781 ttttgtgcaa agctccgtga cggtactgat cggcatgggg gaattcttcg ctatcctggt
     3841 cgccggtatc gacctttcgg ttggcgcgat tctggcgctt tccggtatgg tgaccgctaa
     3901 actgatgttg gcaggtattg atccgtttct cgcggcgcta attggcggtg tactggttgg
     3961 cggcgcactg ggggcgatca acggctgcct ggtcaactgg acgggactac acccgttcat
     4021 tatcaccctt ggcaccaacg ctattttccg tgggatcacg ctggtgatct ccgatgccaa
     4081 ctcggtatac ggcttctcat ttgacttcgt gaacttcttt gccgccagcg taattgggat
     4141 acctgtcccc gttatcttct cgctaattgt cgcgctcatc ctttggtttc tgacaacgcg
     4201 tatgcggctc gggcgcaaca tctacgcact gggcggcaac aaaaactcgg cgttctattc
     4261 cgggattgac gtgaaattcc acatcctggt ggtgtttatc atctccggtg tttgtgcagg
     4321 tctggcaggc gtcgtctcaa ctgcacgact cggtgccgca gaaccgcttg ccggtatggg
     4381 ttttgaaacc tatgccattg ccagcgccat cattggcggc accagtttct tcggcggcaa
     4441 ggggcgcatt ttctctgtgg tgattggcgg gttgatcatc ggcaccatca acaacggtct
     4501 gaatattttg caggtacaaa cctattacca actggtggtg atgggcggat taattatcgc
     4561 ggctgtcgcc cttgaccgtc ttatcagtaa gtaaggaatt gaacatgaaa atctccccct
     4621 cgttaatgtg tatggatctg ctgaaattta aagaacagat cgaatttatc gacaaccatg
     4681 ccgattactt tcatatcgat atcatggacg gccactttgt gccgaatctg acgctctcac
     4741 cgttcttcgt aagccaggta aaaaaactgg cgagtaaacc gctcgactgc catctgatgg
     4801 taacgcgtcc gcaggattac attgctcaac tggcgcgtgc gggagctgat ttcatcactc
     4861 tgcatccgga aaccatcaac ggccaagcgt tccgcctgat tgatgaaatc cgccgtcatg
     4921 gcatgaaagt ggggctgatt cttaatccgg aaacgccggt tgaggccatg aaatactata
     4981 tccataaggc tgataaaatt acggtcatga ctgtcgatcc cggttttgcc ggacaaccgt
     5041 tcattcctga aatgctggat aaacttgccg aactgaaggc atggcgtgaa cgagaaggtc
     5101 tggagtacga aattgaggtg gacggttcct gcaaccaggc aacttacgaa aaactaatgg
     5161 cggcaggggc ggatgtcttt atcgtcggca cttccggcct atttaatcat gcggaaaata
     5221 tcgacgaagc atggagaatt atgaccgcgc agattctggc tgcaaaaagc gaggtacagc
     5281 ctcatgcaaa aacagcataa cgtcgtagcg ggcgtggata tgggggcaac gcatatccgc
     5341 ttttgtctgc ggacagcaga aggtgaaacg ctacactgcg aaaaaaagcg gaccgcagaa
     5401 gttattgctc ccgacctggt gtcgggtatc ggcgaaatga ttgacgagca actcaggcgc
     5461 tttaacgctc gctgtcatgg tctggtgatg ggatttccgg cgctggtcag taaagataaa
     5521 cgcaccatta tttctacgcc taacctgccg ttaacagcgg cagatttata tgatctcgcc
     5581 gataagctcg aaaatacgct gaattgtccg gttgagtttt cccgcgacgt taacctgcaa
     5641 ctctcctggg acgtagtaga aaatcgcctt acgcaacaac tggttctggc agcctatctc
     5701 ggtacgggga tggggttcgc agtgtggatg aacggtgcgc cgtggacggg tgcgcacggt
     5761 gtggcaggcg aactgggcca tatccccctg ggagatatga cccaacactg cgcgtgtggc
     5821 aattctgggt gcctggaaac caattgctct ggaatggcgc taagacgctg gtacgaacaa
     5881 cagccccgaa attacccatt gagcgatctt ttcgtccatg cggaaaacgc ccctttcgtc
     5941 cagagtctgc ttgaaaacgc ggcacgagcc attgccacca gcattaatct gttcgatccc
     6001 gatgcggtga ttctgggtgg tggcgtgatg gatatgcccg ccttcccacg cgagactctc
     6061 attgccatga cccaaaagta cctgcgccgt ccactgccgt atcaggttgt gcgctttatt
     6121 gccgcctcat cttctgactt taatggcgct cagggtgcag caatattggc gcatcaacgt
     6181 tttttgccac agtcctgtgc taaagtccca tgagtaaagc gttggcacag tagcgcaatg
     6241 tcccgctgtg ccaacgacag gtacaatata tataataatt acataaaaca gcccatatta
     6301 ttcccagcaa tgatagttct actgtacttt tccctcttca gcacaaatgc ctaataaaat
     6361 ggcgcgttca gctcacactt ctacttatta aagtcgcaat ttcccaatta tgataacgcc
     6421 agctttaaat gacgtatttt gcatggatta catatttacg acataaatga taaatggaga
     6481 tttatatgaa taactctcgg ttattccgtt tgagcaggat tgttattgcg ttaactgccg
     6541 ccagcagcat gatggtaaat accgctaatg caacagatga agcgaaagcc gccactcaat
     6601 atacccaaca ggttaatcag aattacgcca aatcattacc gtttagcgat cgtcaggatt
     6661 ttgacgatgc tcagcgtgga tttatcgctc cgctgctgga tgaaggtatt ctgcgtgatg
     6721 cgaacggtaa aatttactac cgcgcgaatg attacaaatt tgatattaat gccgctgcgc
     6781 cggaaaccat aaaccccagc ctgtggcgtc agtcgcagat caacggcatt tcaggcctgt
     6841 tcaaagtcac cgataaaatg tatcaggtgc gcggccagga tatctctaac attacgttcg
     6901 ttgagggcga gaaaggcatt attgttatcg acccgctggt gacgccgcct gcagcaaaag
     6961 ccgcacttga tctttacttc cagcatcgtc cgcaaaaacc gattgttgcc gttatctaca
     7021 ctcacagcca cgccgaccac tatggcggcg tgaaaggtat tatctctgaa gccgatgtta
     7081 aatccggcaa agtgcaggtg attgctccgg caggctttat ggacgaagcc atcagcgaaa
     7141 acgtgctggc gggtaacatc atgagccgcc gtgcgctcta ctcttacggt ctgttactgc
     7201 cgcacaacgc gcaaggcaac gtgggcaacg gcctgggcgt gacgctggca acgggcgacc
     7261 cgagcattat tgcaccgact aaaactatcg tcagaactgg cgagaagatg attatcgacg
     7321 gcctggaatt tgacttcctg atgactccgg gtagcgaagc gccagccgaa atgcacttct
     7381 atattccggc cctgaaagcc ctgtgtaccg ccgagaacgc cacgcatacc ctgcacaact
     7441 tctacactct gcgcggcgcg aaaacccgcg ataccagcaa gtggaccgag tacctgaacg
     7501 aaacgctgga tatgtggggt aacgacgcgg aagtactgtt tatgccgcac acctggccgg
     7561 tctggggcaa taagcatatc aatgattata ttggtaaata tcgcgatacc atcaagtaca
     7621 ttcacgacca gaccctgcac ctggcgaacc agggctacac catgaatgaa atcggcgaca
     7681 tgattaagct gccgcctgcg cttgccaata actgggccag ccgcggctat tacggttctg
     7741 tcagccacaa cgcccgcgcg gtgtataact tctatcttgg ctattacgac ggtaacccgg
     7801 ctaacctgca tccgtatggt caggtggaga tgggtaaacg ttacgtgcaa gctctgggcg
     7861 gttctgcccg cgtcatcaac ctggcgaaag aagcgaacaa gcaaggtgat taccgctggt
     7921 cggcagaact gctgaaacag gtgattgccg ccaaccctgg tgaccaggtc gcgaagaatc
     7981 tgcaagcgga taactttgaa cagctgggct atcaggccga gtccgccaca tggcgcggtt
     8041 tctacctgac cggcgcgaaa gagctgcgcg aaggggtaca taagttcagc cacggcacca
     8101 ccggttcccc ggacaccatt cgcggaatgt cggtcgaaat gctgttcgac tttatgtccg
     8161 ttcgcctcga tagtgcgaaa gccgcgggta aaaatatcag cctgaacttc aatatgagca
     8221 acggcgataa cctcaacctg acgctgaacg atagcgtgct taactaccgt aaaacgctgc
     8281 aacctgaagc caacgcctct ttctacatca gccgtgaaga tctgcatgcc gtgctgaccg
     8341 gacaagtcaa aatggtggat ctggtaaaag cgaagaaagc caaaattatt ggcaatggcg
     8401 cgaaactgga agaaattatt gcctgtctgg ataatttcga tttgtgggtg aatatcgtaa
     8461 ccccaaatta atgctactca cttttggggg cgcttaagcc cccaaaagca tttattttaa
     8521 aaatatatac aacaaattaa tacataaaac atataagtta tgaatgcggt ggctgaataa
     8581 atctattttt attcatcgag aaaatagcca tactgacgcc ttcaatggat attgatattc
     8641 actacagata ttgttcgcag cagacaggat gtatttaata tgccaacagt tctctctcgc
     8701 atggcgatgc aactcaaaaa aacagcctgg ataattcccg tcttcatggt ttcgggatgc
     8761 tcattatctc cggcaatccc ggtgatcggc gcttattatc ccggctggtt tttctgcgcc
     8821 attgccagcc ttattttgac gctcatcacg aggcgaatta ttcagcgggc aaatatcaat
     8881 ctggcatttg tcggaattat ttataccgcc ctttttgctc tctacgccat gctgttctgg
     8941 ctggcatttt tctaatctat ttgagacgcg ctcatggaaa gtacgccgaa aaaagctcct
     9001 cgcagtaaat tccctgctct gttagtggtt gcgttggcgc tggttgccct tgttttcgtt
     9061 atctggcgcg tagacagtgc gccatcaact aatgacgctt acgcgtcagc agataccatt
     9121 gatgtggtgc cggaagtcag cggtcgcatt gtagaactgg cggtcaccga caaccaggcg
     9181 gtgaaacagg gtgatttgct attccgcatc gacccacgcc catacgaagc caatctggcg
     9241 aaagccgaag cctccctcgc ggcgctggat aagcaaatta tgctcaccca gcgtagcgtt
     9301 gacgcgcaac agtttggtgc cgactcggtt aatgccacgg tagaaaaagc ccgtgccgcc
     9361 gcgaaacagg ccacagatac attacgccgc accgagccat tactgaaaga aggttttgtc
     9421 tcagcggaag acgttgaccg tgcaagaact gcgcagcgcg ccgcagaagc ggatcttaat
     9481 gccgtattgt tacaggcgca gtcagccgcc agcgccgtca gcggcatcga tgcgctggtg
     9541 gcgcaacgtg cggcggtcga agcggatatt gccctgacca aactgcatct ggaaatggcg
     9601 accgttcgcg cgccgtttga tggccgggtc atttccctca aaacctccgt cgggcaattt
     9661 gcttctgcca tgcgccctat ttttacccta atcgacactc gtcactggta cgtgatcgcc
     9721 aacttccgcg aaaccgatct gaaaaatatt cgctcaggta cacccgcaac cattcgcctg
     9781 atgagcgaca gcggcaaaac cttcgagggt aaagtggatt cgattggcta cggcgtgcta
     9841 ccggatgacg gcggcctggt gctgggcggc ctgccgaaag tgtctcgttc tattaactgg
     9901 gtccgcgttg cccagcgttt tccggtcaaa atcatggtcg ataaacctga cccggaaatg
     9961 ttccgcatcg gcgcttcggc agtcgctaat cttgagccgc aataatgagc gcgctcaact
    10021 ccctgccatt accggtggtc aggctgctgg cgttctttca tgaagagtta agcgagcggc
    10081 ggccaggtcg cgtgccgcag accgtgcaac tctgggtagg ctgcctgctg gtaattctga
    10141 tctcgatgac ctttgagatc ccttttgtgg cgttatcgct ggcagtgctg ttttacggta
    10201 ttcagtcgaa cgcgttttac accaaatttg tcgcgatctt gtttgtggtt gccacggtgc
    10261 tggagatcgg cagcctgttt ttgatctaca aatggtcata cggcgaaccg ttgatccgat
    10321 tgatcatcgc cggaccgatc ctgatgggct gtatgttttt gatgcgcacc catcggttgg
    10381 ggctggtctt tttcgccgtc gccatagtcg ctatttacgg gcaaaccttc cccgccatgc
    10441 tcgactatcc ggaagtggtc gtgcgcttaa ccctgtggtg tatcgttgtc ggcctctatc
    10501 caaccttgct gatgacgtta atcggcgtgc tgtggtttcc cagccgcgct atcacgcaga
    10561 tgcatcaggc gcttaatgat cggcttgatg atgccattag ccacctgacg gacagcctcg
    10621 caccgctacc cgaaacgcgg attgaaagag aggcgctggc gctgcaaaaa ctcaatgtct
    10681 tttgcctcgc ggacgatgcc aactggcgaa ctcagagcgc atggtggcaa agctgcgtgg
    10741 caacggtaac ctacatttac tcgacgctga atcgctacga tcccacctct tttgctgatt
    10801 ctcaggcaat tattgaattt cggcaaaaat tagcctcaga aatcaacaag ctgcagcatg
    10861 ccattgccga aggtcagtgc tggcaaagcg actggcggat cactgaaagt gaagcgatgg
    10921 cggcacggga atgtaacctg gagaatatct gccagacgtt attgcaactg ggtcagatgg
    10981 acccgaatac gccgccaacg cccgccacca aaccgccatc aatggtcgcc gacgctttta
    11041 ccaatccaga ctatatgcgc tacgcggtaa aaacgctgct cgcctgtttg atctgttaca
    11101 ccttctacag cggcgtggac tgggaaggca ttcacacctg tatgctgacc tgcgtgatcg
    11161 tcgctaatcc caatgtcggt tcgtcgtacc agaagatggt gctgcgtttt ggcggggcct
    11221 tttgcggcgc gattctggcg ctgttattca cgttattggt catgccctgg ctggacaata
    11281 ttgtcgaatt gctgtttgtg ctggcaccga ttttcctgtt gggcgcatgg attgccacca
    11341 gctctgaacg ctcttcttat atcggcacac agatggtggt caccttcgcg ctcgccacgc
    11401 tcgaaaacgt ttttggcccg gtgtacgacc tggtggaaat tcgcgatcgc gctctgggta
    11461 tcatcattgg taccgtggtg tcggcggtga tttacacctt tgtctggcct gaaagcgaag
    11521 cgcgcacgct gccgcaaaaa ctggctggcg cgctgggtat gttgagtaaa gtaatgcgga
    11581 tcccacgcca gcaggaagtc acagctctgc gcacttatct gcaaattcgc ataggtctgc
    11641 atgcggcgtt taatgcctgt gaagagatgt gccaacgcgt ggcgctggag cgtcaactgg
    11701 acagcgaaga acgcgcttta ctgattgaac gttcgcaaac ggttattcgt cagggccgcg
    11761 atattcttca cgcctgggat gcaacctgga actcggcgca ggcgctggat aacgcactac
    11821 agccggacag agcaggtcag tttgccgacg cactggagaa atacgccgcc ggtctggcaa
    11881 ccgcactcag tcgttctcct caaataacgc ttgaagaaac acccgcctcg caggccatcc
    11941 tgccgacctt attaaaacag gagcaacacg tctgccagct tttcgcccgc ttgccagact
    12001 ggacagcccc ggcattaacg cccgccacgg aacaggtaca aggagccacg caatgatcaa
    12061 tcgtcaactt tcacgcctgc tgttgtgcag cattctcggc agcacgacgc tgatttccgg
    12121 ctgtgccctg gtacgtaagg attctgcgcc tcatcaacag ctcaaaccgg aacaaatcaa
    12181 actggccgat gatattcatc ttgccagctc cggctggccg caggcgcagt ggtggaaaca
    12241 actcaatgac ccgcagttgg atgccctgat ccaacggacg ctaagtggtt cacacaccct
    12301 cgccgaagcg aaactgcggg aagaaaaagc gcagtcgcag gccgatttgt tagatgccgg
    12361 ttcacaatta caggtcgcag cgttagggat gctcaaccgc caacgcgtct cggcgaacgg
    12421 ctttttaagc ccttatgcga tggatgcgcc agcactgggt atggacgggc cgttctatac
    12481 ggaagccaca gtcggtttgt ttgccggact ggatctcgat ttgtggggtg tgcatcgctc
    12541 agcggttgct gccgccattg gcgcgcataa tgccgcgctg gcagaaaccg cagcagtaga
    12601 gctatcgctg accacgggcg tagcgcagct ttattacagt atgcaggcca gctatcagat
    12661 gctcgatctg ttagaacaaa cacgcgatgt gattgattac gcggtgaaag cgcaccagag
    12721 taaagtggcg cacggtctgg aagcgcaagt gcctttccac ggcgcgcggg cacagattct
    12781 ggcggtcgat aaacaaattg ttgccgttaa agggcaaatc accgaaacgc gagaatctct
    12841 gcgtgcattg attggcgcgg gagccagcga tatgccggag atcaaaccgg tggcattacc
    12901 gcaagtccag accggcattc cggcgacact ctcttatgag ttgctcgcca gacgcccgga
    12961 tctgcaagcg atgcgctggt atgttcaggc gtcattagat caggtggatt ccgcgcgggc
    13021 gctgttctat ccgagctttg atatcaaagc gtttttcggc ctggactcta tccacctgga
    13081 taccttattc aaaaaaacca gtcgccagtt caactttatc ccgggtctga aattgccgct
    13141 gtttgacggt ggacggttga atgccaatct cgaaggcact cgcgccgcca gcaacatgat
    13201 gattgaacgt tacaaccagt cagtactgaa tgcggtgcgc gacgttgccg tcaacggcac
    13261 gcgtctgcaa acgctcaacg acgagcgaga gatgcaggct gaacgcgtgg aagcaacgcg
    13321 ctttaccctg cgcgctgccg aggccgccta tcagcgcggc ttaaccagcc gcttacaggc
    13381 caccgaagcc cggttgccag tacttgccga agagatgtca ttactgatgc tggacagccg
    13441 ccgggtgatc caaagcattc agttgatgaa atcgctgggc ggcgggtatc aggcaagtcc
    13501 cgtcgtcgag aaaaaataaa atgtctgccg cgtgatggct gtcacgcggt atttcgtttc
    13561 gtcacgtcaa aactgacgac agcctgtttt tcgtcagagt tttgaataaa tagtgcccgt
    13621 aatatcaggg aatgaccaca aataaaatgt ggcataaaag atgcatactg tagtcgagag
    13681 cgcgtatgcg tgatttgatt aactggagcg agaccgatga aaaaagtcgt cacggtttgc
    13741 ccctattgcg catcaggttg caaaatcaac ctggtcgtcg ataacggcaa aatcgtccgg
    13801 gcggaggcag cgcaggggaa aaccaaccag ggtaccctgt gtctgaaggg ttattatggc
    13861 tgggatttca ttaacgatac ccagatcctg accccgcgcc tgaaaacccc catgatccgt
    13921 cgccagcgtg gcggcaaact cgaacctgtt tcctgggatg aggcactgaa ttacgttgcc
    13981 gagcgcctga gcgccatcaa agagaagtac ggtccggatg ccatccagac gaccggctcc
    14041 tcgcgtggta cgggtaacga aaccaactat gtaatgcaaa aatttgcgcg cgccgttatt
    14101 ggtaccaata acgttgactg ctgcgctcgt gtctgacacg gcccatcggt tgcaggtctg
    14161 caccaatcgg tcggtaatgg cgcaatgagc aatgctatta acgaaattga taataccgat
    14221 ttagtgttcg ttttcgggta caacccggcg gattcccacc caatcgtggc gaaccacgta
    14281 attaacgcta aacgtaacgg ggcgaaaatt atcgtctgcg atccgcgcaa aattgaaacc
    14341 gcgcgcattg ctgacatgca cattgcactg aaaaacggct cgaacatcgc gctgttgaat
    14401 gcgatgggcc atgtcattat tgaagaaaac ctgtacgaca aagctttcgt cgcttcccgt
    14461 acagaaggct ttgaagagta tcgtaaaatc gttgaaggct acacgccgga gtcggttgaa
    14521 gatatcaccg gcgtcagcgc cagtgagatt cgtcaggcgg cgcggatgta tgcccaggcg
    14581 aaaagcgccg ccatcctgtg gggcatgggt gtaacccagt tctaccaggg cgtggaaacc
    14641 gtgcgttctc tgaccagcct cgcgatgctg actggtaacc tcggtaagcc tcatgcgggt
    14701 gtaaacccgg ttcgtggtca gaacaacgtg cagggtgcct gcgatatggg cgcgctgccg
    14761 gatacgtatc cgggatacca gtacgtgaaa gatccggcta accgcgagaa attcgccaaa
    14821 gcctggggcg tggaaagcct gcctgctcat accggttatc gcatcagcga gctgccgcac
    14881 cgcgcagcgc atggtgaagt gcgtgccgcg tacattatgg gcgaagatcc gctgcaaact
    14941 gacgcggaac tgtcggcagt acgtaaagcc tttgaagatc tggaactggt tatcgttcag
    15001 gacatcttta tgaccaaaac cgcgtcggcg gcggatgtca ttttaccgtc aacgtcgtgg
    15061 ggcgagcatg aaggcgtgtt tactgcggct gaccgtggct tccagcgttt cttcaaggcg
    15121 gttgagccga agtgggatct gaaaacggac tggcagatta tcagtgaaat cgccacccgt
    15181 atgggttatc cgatgcacta caacaaaacc caggagatct gggatgagtt gcgtcatctg
    15241 tgcccggatt tctacggtgc gacttacgaa aaaatgggcg aactgggctt cattcagtgg
    15301 ccttgccgcg atacttcaga taccgatcag gggacttctt atctgtttaa agagaagttt
    15361 gataccccga acggtctggc gcagttcttc acctgcgact gggtagcgcc aatcgacaaa
    15421 ctcaccgacg agtacccgat ggtactgtca acggtgcgtg aagttggtca ctactcttgc
    15481 cgttcgatga ccggtaactg tgcggcactg gcggcgctgg ctgatgaacc tggctacgca
    15541 caaatcaata ccgaagacgc caaacgtctg ggtattgaag atgaggcatt ggtttgggtg
    15601 cactcgcgta aaggcaaaat tatcacccgt gcgcaggtca gcgatcgtcc gaacaaaggg
    15661 gcgatttaca tgacctacca gtggtggatt ggtgcctgta acgagctggt taccgaaaac
    15721 ttaagcccga ttacgaaaac gccggaatac aaatactgcg ccgtgcgcgt cgagccgatc
    15781 gccgatcagc gcgccgccga gcagtacgtg attgacgagt acaacaagtt gaaaactcgc
    15841 ctgcgcgaag cggcactggc gtaatacagt cctttctaca gcctcctttc ggaggctgtt
    15901 tttttatcca ttcgaactct ttatactggt tactccctac ccaatcgtat tatcaaaatg
    15961 aaaaaaatta tcgcattgat gttgtttttg acattctttg cccacgcgaa cgactccgag
    16021 cctggcagcc agtatttaaa ggcagcagag gcaggggacc gacgcgcaca atattttctt
    16081 gccgacagct ggtttagctc cggcgatttg agcaaagccg aatattgggc acagaaagcc
    16141 gccgacagcg gtgatgctga tgcctgcgcg ctgttagcgc agatcaaaat caccaatccg
    16201 gtcagtctgg actatccaca agcaaaagtt cttgcagaga aagcggcgca agcgggcagt
    16261 aaagaaggtg aagtaacgct ggcgcatatt ctggtaaata cgcaagcggg taaaccggat
    16321 tatccaaagg caatttcgct gttagaaaac gcctcggaag atctggagaa cgactctgcc
    16381 gtcgatgccc aaatgctgct tggtttgatt tacgccaacg gcgtgggcat taaggccgac
    16441 gatgacaagg caacctggta tttcaaacgc agctctgcga tttcccgaac cggttattcc
    16501 gagtactggg cgggaatgat gttcttaaac ggtgaagaag gttttatcga gaagaacaaa
    16561 caaaaggcgc tgcactggtt aaatctgagc tgtatggaag ggtttgatac cgggtgtgaa
    16621 gagtttgaaa aattaactaa cggttaaggt aggcctgata agacgcggta agcgtcgcat
    16681 caggcattga tgtcggatgc ggcgtaaacg ccttatccga cctacaaaat cacgctaaat
    16741 caggctattg cgcagtctgg cgtaggcctg ataagacgcg gtaagcgtcg catcagatat
    16801 tgatgtcgga tgcggcgtaa acgccttatc cgacctacaa aatcacccta aatcaagcta
    16861 ttgcgcagtc tggcgtaggc ctgataagac gcggcaagcg tcgcatcaga tattgatgtc
    16921 ggatgcggcg taaacgcctt atccgaccta caaaatcacc ctaaatcaag ctattgcgca
    16981 gtctggcgta ggcctgataa gacgcggtaa gcgtcgcatc aggcatccac atattgccgg
    17041 gtggatatcc ccggcaatct tcaattattg atccgcagtt ttatcaaatt tgcccagcac
    17101 ttcgcgctca taagccagcg ctttcttacg gtcaaatttg tgttcccact tggcaatcac
    17161 cagcaccgcc agcgcattac ccaccacgtt cagcgcagta cgcgccatgt cgaggatacg
    17221 gtcaacaccg gcaataaacg ccagaccttc cagcggaata cctacgctac ccagcgttgc
    17281 cagcaacacc acaaacgaca cgcccggcac gccagcaatc cctttcgagg tcaccatcag
    17341 cgtcagcacc agaatgattt cctgccagat ggacaggtca atgccataca actgcgcgat
    17401 gaaaatagcg gcaatacttt gatacagcgt cgaaccatca aggttaaaag agtaaccggt
    17461 cggcaccacg aaactggtga tcgacgccgg tgctccgtag gcttccatct tctcaataat
    17521 tcgcggcagc acgctttcag agctggcagt ggagtacgcc agaatcagct catctttcag
    17581 aatacgaatc aggatccaga cgcttaaccc gcacaggcgc gccacaattc ccagcactac
    17641 cagcgcgaag aacagaatgg cgaaatgcac cagcagcacc agtttcgcca gtggccacag
    17701 agacgagaaa ccaaagttag ccaccgtcac cgcaatcagc gcaaacacac ccaccggcgc
    17761 ataacgcatc accatgtgag tcactttaaa catggtttca gagatggagc ggaaaacggt
    17821 caccagcggt tcacgatgcg tcgcgggcag ggatgaaagc cccagaccaa acagcaccga
    17881 gaagaagatg atcggcagca tttcgccttt cgccatcgac gccacaatgt tcgtcggcac
    17941 cagcgacaaa atcgtgccca taatgccgtg ggaactgctt tgtaccgctt ccgtagtgct
    18001 ctgatatttc gagatatcga cggtcgccag ctgcgacata tccaccccgg caccgggctg
    18061 gaagacgttc gccagagtga tccccaaaat gatggcgacg gtggtgatca cctcgaagta
    18121 aataatggtt ttcgcgccaa tacgcccgag ctgtttggca tcaccaacac ccgcgatacc
    18181 caccaccagc gtggagatca caatcggcac aacaatcatt ttgatcagat ggatgaagat
    18241 atcacccgcc ggagagagca aattgacgac cagccagtcg cggctgtcgc tatggtagtg
    18301 caggtagctt cccaggagaa tgcccagcac catagcaaac agaatctgcc aggccaggct
    18361 gaattttata tttttcatca gaataacttc ctcaatggag cgccgttccc cgttcagaaa
    18421 cgcgagaacg tgacatactg ccagggcatg aattgtgttg cgttggtttg tagatttttt
    18481 taacgcttat gatcagtatc atttgcgcgg tatgttgcgc aaccctttaa caacagagct
    18541 tttcgctgca ttaatgcata ataacgcgag tttatggcag gattaatgaa taaccatttg
    18601 ttatggaaag atttttattt gtttaatctg caataatact gattggtgat tatttttggc
    18661 atatgttcat tcgattattt tttatgcagc tcaataacat aatcttcatc accagatgat
    18721 taatctgatc tccgctgcaa caatttcgcc atattaatcg actcgaccag ctgtgtccgg
    18781 ttgatccggg gtgagttgag atccatcact ttttgccata gttcgatggc ctgcgcgtaa
    18841 ttcgcctgca taaacgcatc ggaagccagc agcatcagag cggtgatttc gttagagtcc
    18901 agcgcgaggg ctttatcgat cattgcgcgt gtctgggcgg tcatatgctg gctggcctgg
    18961 taataaagca ccgtcgccag cgccgcatac agttcagcgt tctcaccacg tagttgcaac
    19021 gcctgacggt acgccagcag cgaattgctg taatcatttt gccacagata gtactcgccc
    19081 agtaacgccc actgttcgct gttttgtgga ttagcgcgga ttttatcctg caatgcctga
    19141 agctgcgctt ctggggtttg ctggctggca aactgatgca gcggatcgcg ctgacgctga
    19201 tactccgcac gtaccgcctg ccattttggg cttaacagat aactgccgat acagagaaat
    19261 gccatcaaaa tcgtgagtgt tgtcagcatt ttcaccggta tttgtgactg tttcatcgct
    19321 tcgccctcac tcgccagagg atcagtgcca tcagcagtaa caacaccact ggcagcgccc
    19381 acaacaccag cgtctggccc gttaacggtg ggttatagcg aacaaaatct ccgtagcgtt
    19441 cggtcatcca gccgatgatt tcagcttcgt tcttcccctc cgccaccatg ctgtaaacct
    19501 gatggcgcat actgaccgcc accggcgcgt tggattccag taagttttga ttctggcact
    19561 gcggacaacg taactggctg gcaatattta acgcctgttg ctgttgttgc ggattggcga
    19621 attgccaggt gtctacgacc tgagcgcgcg caaaacaggt aaataacagc agtagcgtga
    19681 gaagtccttt attcatcgcg cttcctcccc cgccatccgc ttaacaatgc gcccgcaatc
    19741 atcaacaatc ctccccccca gatccagcgc acaccgcttt gtacatacaa acgaaaagcg
    19801 taacgatccg ctccggtttt ctcgcccatg accgcatacc agtcatggat gccgttccag
    19861 cgaattgacg gttccatcat ttgctgacgg cgtgcttcat aaaaacgccg ctccggcgtc
    19921 agttcaccaa tgcgttgctg atggtcaaac agcgccacta tcgctttttc gctggtgtaa
    19981 ttgcctctgg cttgcagatc gagtcgctca aaacggaagg tgtatcctgc cagcgtcacc
    20041 tgctgacccg gctgtaaatt caggctgatc tcctgacgac tgacgctgga aaccacgatc
    20101 cccgcggcaa ataacagcac gccagcatga gctaccagcg ccggaagctg cacgcgtttg
    20161 ccagagacaa acgtcgccag cacaatcacc accagcatca acagaccaaa cggtaacgtc
    20221 gcgcggttaa aatacggcgc gccaacggag aggcgtcccc agcccagcag accgtaaatc
    20281 atcggataaa gcgttcccac cagcacgatc agtagcactg cgcaaaacag caacagcgta
    20341 gcgaggatta acatttcccg cgataacccc gaaaaacgca ccgccgggcc accgtcccgc
    20401 gctcgccagc catacagagc cagagacgca aggctaatca gtgcaaacag gctgaacaac
    20461 ggcacggcgc ggacgttatc cagtgcgaac gcatgaaccg aaaccagaat gccagaacgg
    20521 acaattaagg tgcccagcag cgacagcatc agagtaacta tcgccagcag cagcgaccag
    20581 tggcggaaaa tcccccgctg gcgtgtcagg gacaaactgt gcagcagcgc agtggcagaa
    20641 agccagggta ataaagaggc gttttccacc ggatcccaga accaccagcc gccccagccc
    20701 agttcgcaat aggcccacca ggaaccgagg atgatcccag ccgttaatgc actccagcca
    20761 ggtaacgccc agcgccagca aattcgggcg caggcaccat caaactcgcc gcgcagtaaa
    20821 ctcgccagcg ccacgctcgc cgccaccatc aaaccgccat agccaaggta aagcagcggt
    20881 ggatgaaaga taagaccggg atgttgcagc atcggattga gatcgcggcc ttcgattgct
    20941 ggtggaaata tccgcacgaa gggatcggac cacagcacta caaacagtag cagtgcggcg
    21001 agcatgagag ataaaacggc tagcgttagc ggaaatagcg gatcggtttg ctgccgataa
    21061 tgccaggcaa acagcgcgct ccaggcggaa agcagcagca cccaaagcag cagcgaacct
    21121 tcatgaccgc cccacaccgc cgccagtttg agttcccacg acagcaggct gtagctatgt
    21181 tgggcgacat aaatgaccga gaaatcgctg atgagaaaac aatacgtcag tacgccaaag
    21241 gcgagcagca ggagcgcgaa ctgcgccaga atgccgatgc aagtgagtcg catcatggca
    21301 ggccagcgca accgcactcc cgcgaaggcc gtcaacgggg tcaacacgtt gaccccgaga
    21361 cttaacaaca acgccagaaa accgacttca ggaaggaata cgtccagaaa ccacctcttt
    21421 tctggcttac gccaccgtca actgtccggc gtagagaatg aaaaagcgca gcatcaacac
    21481 gccgaccaga ctggccccac acgccgccag cacggtagga atgccggaac tgcgattgac
    21541 ccacggtttg agcaacattg gcacaatcag ccccagcccg gcgacaccaa gccagaacca
    21601 ccacgtccag aaaccgccac ctaatgccgc caccagcgca cgcactttac cgtcatcgcc
    21661 cagcgccaga ccgacaaaaa acgccaccag caggaagatt tcaccccata ccacagggat
    21721 ttccatacgg tgaacaaagt gcgcttccgt ggaatgcgga tttttgcgtt ggcgtatcgc
    21781 catggcgatc agcgctaccg ccgcaccgga cgagatgccg gagaagagga acagcaccgg
    21841 caggatcggg ttattgagga acggatacga tttcagcgcc gacagcagaa agccggtata
    21901 agcccccagc agcaccgcca gcaccagcat caatgtttcc agtccgcgat gaacgggcgt
    21961 cagcaggctc agaacctttt gcacgatccc cagcttcggc aaccagcgtt gttgcagggc
    22021 aagcaaatca tgttcaaaga ttttcgccag ccacagcacc agcaccacca tgtagagctg
    22081 aaacaacatc acccccatcg acatcaccga ggtaaaactg tagtggaaca tcagcttcca
    22141 gaaggtccac ggctttgtca ggtggaagac gaggatcaac agaccgagga tcaccgcgcc
    22201 aggcccgaca attagcgtgg tgcgcagcaa cgtactgtct gcaccgcccg cctgcgggta
    22261 gaagcgacgt aacagcacgg ccagcgtcac cagaccggca gaaataccaa tcaaaaacag
    22321 gtagatggca atcggccagt cccacaccag cgattcaaaa tgaaatgcgg aagtctgcat
    22381 cattggctca cctccccgta tttaaacggt acgcggtaca gcttcggttt ggttcccagc
    22441 gccagcttgt agcggtaagt gggcttctgg cgcagcagtt gcgaaatctc actgttggga
    22501 tcgtccagat tgccaaacgt cagtgccttg gtcgggcaag cttcaacgca cgcgggcagc
    22561 ttaccggcct gcaaattggt cttacggcag aaatcgcatt tgtccgccgt cttcgtgacc
    22621 ggatggataa agcgcacgcg gtacggacag gcggcgatgc agtactgaca accgacgcag
    22681 agatcaggat taacatcgac aatcccgcta gcggcatcac gaaaagacgc accggtcggg
    22741 cagacgtcaa cgcacggcgc atgatcgcag tgctggcaag acttacggaa gaagcgatat
    22801 ttcacatcag gaaattcgcc ctgcggctcg ctacgaataa tcgtcaggcg cgagacgcct
    22861 tccggcacct tgttcacttc ccgacaagca tccatacagg cggtacagcc gatgcataac
    22921 gactcgtcat gcaccatgcc gtaacgcacc ccattgatat tcaacgtttt cgccaccacg
    22981 cgccctgcgg tgccgctgac ggctgccagc acgccgacgc cggtgagaaa ctggcgacga
    23041 gaccaggtca tggctgctcc ttaagcaacg gaacagacgc cgggttaaag ttcggattgg
    23101 tgcgctgatc gctgtggcaa tcgacgcaaa tcttaatccg tcctttgtca cttaacgtct
    23161 gcatcgtatc ttgctgtgga tgcagggaat ggcagctggc gcacgccact ttggttacgt
    23221 ggacatcgtg tggccagaac gctttttgca actgttccgg cagatggcag gacatacaga
    23281 cgctgttctg ctctccaacc ttgtacatcg gctcgttaaa gcgcatcaca tctttcaccc
    23341 cttcgcggtg ttgcggtgat ggctgaccgt ggcagttggt gcaggtgacc ggcagtttgt
    23401 tattcgggtt gataacggaa gcatgtttgc catgcatacc ttcggtatct ggcttatgac
    23461 agtccagaca ggcggcatcc gggttacgct gctgggtaac ttcgtagcga tcaccagatg
    23521 cctgcgccgc aggggtagcg gtaattccgt tcaggctcca caacaggccc gacgccagca
    23581 ccccggcagt taataacgaa cgtaatacgc tcatattcac tccattgtgt tttccacccc
    23641 ttgcgaggcg gaacggggtt attggcttaa cagaccgttt ttacgtgcct gctcttccca
    23701 ctgcgggatc accgttttga tgaagtcctg cttctcggcc ttgatttgct ccatgttcag
    23761 gccaatggcc tgctgggctt tctctttggt cgagatatcc gggatctgga tttcatgggt
    23821 aatgcctttg gtcgccagca ggcgcgccag tttggtgcgt gcatccgccg ctttatccat
    23881 cgccgtaccg agcatcctca gaccttcttc cggtgcgtgc atatgaatgc cgtgggaagc
    23941 gatcgccaaa tcccaacgcc actgggcatg acggatatcg tcctgaatcg gcttcatttc
    24001 ggcttccgtt gcgcctgcat ccagcgccgc tttcgcttcg aagtgagcgt gaaccagttg
    24061 atcttcaacc ttgattttca ggtcgttaat cgactgctta cgttccgcga ccactttttg
    24121 caaggcagct ttgtcctggg tatggcagtt cgcacaagtc tgggcgaagt tatcaaacgg
    24181 attaccaatt ttatggtcgg tgtagagttt gccttcggcg ttctgcactt ttggcatatg
    24241 gcaatcgata caggtcacgt tgtttttgcc gtgaatgccc gctgtccagg tttcatattc
    24301 cgggtgctgc gctttcagca ttggcgtttt cgacagggag ttagtccagt cagagaaggc
    24361 aattttgtcg taatactgct ccatattttc gactttcatg ccgtcatccc acgggaattt
    24421 aaccgctttg tttttgccgt cgaagtaata ctccacatgg cactgaccgc aaaccatcga
    24481 ttgctggtcg aaacgtccgg ctttctcaaa aggtttacca atggcttcca tcgcgcgagc
    24541 cgcatacgga cgggaaaggg ttaactccgg tttgcctttg gcgaactctg gtgaggcggt
    24601 gttatggcaa tcggcacaac ctaagttgtt gacgatttcc gggccgccgc gcgcccattt
    24661 tccgtggaag tagccatctt cgccgtcttt ctggatcaga cgcgccacat ctgggctttt
    24721 acaactccag catgccatcg gtagcggacc atcttcagcg tttttcggcg caccggtacg
    24781 cagggtttca cgcacatcgg tcacagcaaa ggcatgtcca cgcggcttgt tgtaatcgcg
    24841 cgagaaggga taccccgccc acaggatcac cagccgtgga tcttccgcca gggcgtcaac
    24901 acgctctgac tgttccgagg tggctttcca ggagagatat tgatcgggat gttgcggggc
    24961 aaaggtttca ttcttcgctt caacagttac aggttttgca ggagctgccg tttgttcagc
    25021 gtgaacagaa gtgaaaaaga aaaaaggaat caataagctg aagatacggc gtgcgtttat
    25081 ttttatcctt gtcatagggg cttcatccga attgcgccat tgttgcaatg gcggttttta
    25141 ttgtttttca cgacagtaac cgcacctaca ctgtcatgac attgctcgcc cctatgtgta
    25201 acaaataacc acactgtgaa tgttgtcttt aatcaattgt aagtgcatgt aaaataccac
    25261 tttagagtta gtcagtatct tcctcttttt caacagcatg cataactgca tgttcctcaa
    25321 agaattaatc aacttttgtt gctgaccttc aaaaattacc ctgccgttta tttgcacaat
    25381 tctacttttg cgtgatctgt cgcccaaata ctaaacaaaa ctgccaatac ccctacattt
    25441 aacgcttatg ccacatatta ttaacatcct acaaggagaa caaaagcatg agtcaaattc
    25501 acaaacacac cattcctgcc aacatcgcag accgttgcct gataaaccct cagcagtacg
    25561 aggcgatgta tcaacaatct attaacgcac ctgatacctt ctggggcgaa cagggaaaaa
    25621 ttctcgattg gatcaaacct taccagaagg tgaaaaacac ctcctttgcc cccggtaatg
    25681 tgtccattaa atggtacgag gacggcactc tgaatctggc ggcaaactgc cttgaccgcc
    25741 atctgcaaga aaacggcgat cgtaccgcca tcatctggga aggcgacgac gccagccaga
    25801 gcaaacatat cagctataaa gagctgcacc gcgacgtctg ccgcttcgcc aataccctgc
    25861 tcgagctggg cattaaaaaa ggtgatgtgg tggcgattta tatgccgatg gtgccggaag
    25921 ccgcggtcgc gatgctggcc tgcgcccgca ttggcgcggt gcattcggta attttcggcg
    25981 gcttctcgcc ggaagccgtt gccggacgca ttatcgattc taactcacga ctggtgatca
    26041 cttccgacga aggcgtgcgt gccgggcgta gtattccgct gaagaaaaac gttgatgacg
    26101 cgctgaaaaa cccgaacgtc accagcattg aacatgtggt ggtactgaag cgtactggcg
    26161 ggaaaattga ctggcaggaa gggcgcgacc tgtggtggca cgacctggtt gagcaagcca
    26221 gcgatcagca ccaggcggaa gagatgaacg ccgaagatcc gctgtttatt ctctacacct
    26281 ccggttctac cgggaagccg aaaggcgtgc tgcacactac cggcggttat ctggtgtacg
    26341 cggcgctgac ctttaaatat gtctttgatt atcatccggg tgatatctac tggtgcaccg
    26401 ccgatgtggg ctgggttacc ggacacagtt atttgctgta cggcccgctg gcctgcggcg
    26461 cgaccacgct gatgtttgaa ggcgtaccga actggccgac gcctgcccgt atggcgcagg
    26521 tggtggacaa gcatcaggtc aatattctct ataccgcgcc cacggcgatc cgcgcgctga
    26581 tggcggaagg cgataaagcg atcgaaggca ctgaccgttc gtcgctgcgc attctcggtt
    26641 ccgtaggcga gccaatcaac ccggaagcgt gggagtggta ctggaaaaaa atcggcaacg
    26701 agaaatgccc ggtggtcgat acctggtggc agaccgaaac cggcggtttc atgatcaccc
    26761 cgctgcctgg cgctaccgag ctgaaagccg gttcggcaac acgtccgttc ttcggcgtgc
    26821 aaccggcgct ggtcgataac gaaggtaacc cgctggaagg ggccaccgaa ggcagtctgg
    26881 tgatcaccga ctcctggccg ggtcaggcgc gtacgctgtt tggcgatcac gaacgttttg
    26941 agcagaccta cttctccacc ttcaaaaata tgtatttcag cggcgacggc gcgcgtcgcg
    27001 atgaagatgg ctattactgg atcaccgggc gcgtggacga cgtgctgaac gtctcgggtc
    27061 accgtctggg aacggcggag attgagtcgg cgctggtggc gcatccgaaa atcgccgaag
    27121 ccgctgtcgt cggtattccg cacaatatta aaggtcaggc gatctacgcc tacgtcacgc
    27181 ttaatcatgg ggaggaaccg tcaccagaac tgtacgcaga agtccgcaac tgggtgcgta
    27241 aagagattgg cccgctggcg acgccagacg tgctgcactg gaccgactcc ctgcctaaaa
    27301 cccgctccgg caaaattatg cgccgtattc tgcgcaaaat tgcggcgggc gataccagca
    27361 acctgggcga tacctcgacg cttgccgatc ctggcgtagt cgagaagctg cttgaagaga
    27421 agcaggctat cgcgatgcca tcgtaaccca caattgcccg atgcgagtcg gtaacggttt
    27481 gtaggcctga taagacgcga cagcgtcgca tcaggcgctg gttgccggat gcggcgtgaa
    27541 cgccttatcc ggcctacatt cggcaacggc tgtaggcctg ataagacgcg acagcgacgc
    27601 atcaggcgtt gattgccgga tgcggcgtaa acgccttatc cggcctacat tcggcaacgg
    27661 ctgtaggcct gataagacgc gacagcgtcg catcaggcgt tgattgccgg atgcggcgtg
    27721 aatgccttat ccggcctaca ttcggcaagg gttacccaag cgttaacctt ctcccataag
    27781 ggagcgggaa ttaaaacaat ccctacatta cctctggaga atctgtgatg aatggtacga
    27841 tttatcagcg gatagaagac aatgcgcatt tcagggagtt agtcgaaaaa cggcaacggt
    27901 ttgccaccat cctgtcgatt attatgctgg cagtttatat cggctttatt ttactgatcg
    27961 ccttcgcgcc cggctggctg ggcaccccgc tgaatccgaa caccagcgtc acacgcggta
    28021 ttccgattgg tgttggagta attgtgatct cctttgttct cactggtatc tacatctggc
    28081 gggcgaacgg cgaattcgac cgtcttaata atgaagtcct gcatgaggta caagcatcat
    28141 gaaaagagtt ctgacggcgc ttgccgccac actccctttc gcagctaacg ccgcggatgc
    28201 tattagcggg gccgtagagc gccagccaac aaactggcag gcgattatta tgttcctgat
    28261 tttcgtcgtg tttacgctcg gcattaccta ctgggcgtca aaacgcgtac gttcgcgtaa
    28321 tgactactac accgcaggcg gcaatatcac cggttttcag aatgggctgg cgattgccgg
    28381 ggactatatg tccgccgcct cattcttggg gatctccgcg ctggtgttta cctccggcta
    28441 tgacggctta atttactcgc tgggcttcct ggtaggctgg ccaatcattc tgttcctgat
    28501 tgccgaacgt ctgcgtaacc tgggccgcta cacctttgcc gatgtagcct cttaccgcct
    28561 gaaacaaggg ccgattcgta ttctttcggc ctgtggttct ctggtggtgg tggcgcttta
    28621 ccttatcgcc cagatggtgg gcgcaggtaa actcatcgag ctgctgtttg gccttaacta
    28681 tcacattgcg gtggtgctgg tcggcgtgct gatgatgatg tacgtcctgt tcggcggcat
    28741 gctggcgacc acctgggtac agattatcaa agccgttctg ttgctgttcg gtgccagctt
    28801 tatggccttt atggtgatga aacacgtcgg ctttagcttc aacaatctgt tcagcgaagc
    28861 gatggcggta cacccgaaag gtgtcgatat catgaagcca ggcgggctgg tgaaagatcc
    28921 gatctcagca ctgtcgctgg ggctcggact gatgtttggt acggcgggct tgccgcatat
    28981 tctgatgcgc ttctttacgg tcagcgatgc ccgcgaagca cgtaagagcg tgttctacgc
    29041 caccggattt atgggctact tctacatcct gacctttatt atcggcttcg gcgcaatcat
    29101 gctggttggt gcgaatccgg aatataaaga tgctgcgggc catctgattg gtggtaacaa
    29161 catggcggca gttcacctgg ccaatgcggt gggcggcaac ctgttcctcg gctttatttc
    29221 agcggttgct ttcgccacta tcctcgcggt ggtagcgggg ctgacgctgg cgggcgcatc
    29281 ggcggtgtct cacgacctgt acgctaacgt cttcaaaaaa ggcgcgaccg aacgtgaaga
    29341 gctgcgggta tcgaaaatca ccgttctgat tttgggtgtg attgccatta tcctcggcgt
    29401 gctgtttgag aatcagaaca tcgcctttat ggtggggctg gcgtttgcca tcgcggcgag
    29461 ctgtaacttc ccgatcattc tgctttccat gtactggtcg aaactgacta cccgtggcgc
    29521 aatgctgggc ggctggttag gactgattac tgcggtggtg ctgatgatcc tcggtccgac
    29581 gatttgggta cagatcctcg gtcacgaaaa agccatcttc ccgtatgaat acccggcact
    29641 gttctctatc agcgtggcat tcctcggtat ctggttcttc tcggcaacgg ataattcagc
    29701 ggaaggtgcg cgcgagcgtg aactgttccg tgcgcagttt atccgctccc agactggctt
    29761 tggcgttgag caaggccgcg cgcattaatc tccactagcc tctggtcacc tgaccaacgg
    29821 taaaaaggtg cgatgtactg caccttttta ccgattacac cgccaaagcg ttattggttc
    29881 tcttcaatca gcaacgcttc aagcaaatcg agatcgtgca gtaatttttg cagcgtttcg
    29941 ttactgatct cccgcgtggc gcgcaggtgg tacagttcag cacgttcaga acgcaatgcc
    30001 gccagacgga agcgacgctc aaggttctct tcctgcacgg aactttcaac gtcgttacgt
    30061 ccatcggcgc gacgacgcag gttaccaatg acgcgagaac taacctctgt gagcagctgg
    30121 ttatcgatat tctcttcggt atccgccgcc agacgctcct ccattttctg gatcgccaca
    30181 atcgccactt ctgccgttgc cgctcgcgca atacgttcct ctttcaattg ttgcgaatgg
    30241 tccgcgactt caatgtgttg tagcagaata ggcaacatca ccacgccgac aaacagcgag
    30301 aagagaatga caccagccgc caggaacacc agctcatagc gcgccgggaa gacgttacca
    30361 tccggcaaga gcagcgggat tgagagcaca ccagccagag tgatcgcccc acgcaccccg
    30421 gcgaaagacg cgatcaggat ttctcgtgtg gtccacgaac caaactccat cggcttcttc
    30481 ttcaggaagc ggttgctgaa ctttttcatc gtccacagcc agccgaaacg gaccagcatc
    30541 agcgccgcat aaatcagaat gatatcggta aacagcatcc agatttcgac gttagggtcg
    30601 atttctgccg ccatcagcga cgtctccaga atacccggca gctgcagacc taacagcagg
    30661 aataccatgc cgttaaagac aaattccagc atcgcccagg tactgtttgc gcgcaggcgc
    30721 attgccagcg gcgcacggcg catcacaccg gaacgggtga tggtcatccc ggcggcaacc
    30781 gcagcgagga taccggaaac gccaatatgt tcggcaatca gataagaagc gaatggcagc
    30841 aggaacagca ggacgatctg cgtcgcgggt tcatcaccgc cccagcggct gaggaatcgc
    30901 agcgagcgac cgtacagcca gctcaccaca aaaccggcga gaataccgcc aatggccact
    30961 ttcataaatt cgaccgtcgc accaccgacg gtgaagatca tcgtccccat cgccactgcc
    31021 acggcaaact tcaacgacac cagaccggag gcgtcattca tcaacgcttc gccctgcaaa
    31081 atgcccatga tttttttcgg gatgcgccct tcgccaacaa tccccgagag cgccacagca
    31141 tcggtcggag aaagcaccgc cgccagcgca aaggcgggga tcagcggaat gcccggcacc
    31201 acccagtaaa taagaaagcc aatgccgacc acggtgacca ccaccagcgc cagcgcgagg
    31261 ccgaaaatct ctcgaccatg ttcgagaaat tcacgggtcg gcgttttcca gccatcagcg
    31321 aacagcaacg gcgggataaa caagactaaa aagagttcag gatcaaactc cacatgcaaa
    31381 ccaaacgtcg gccacgccag tagcgcaccg atggcgattt gcataagcgg aagcgggatc
    31441 tgaaagggca tgacacgagt gaccaccccg gacagcgaga ccacgagggt cattatcagt
    31501 atggtgaaga agatttccat gcgttccctg tttgcgatgt tttttatgat ctgagaaagg
    31561 cgttatgcct gaattctcat tttttcagaa tatcgcaaaa gctcacacaa tgtgcggaga
    31621 aaataaaaac gggcagccat tgccacccgt tttcagaatc atatgaaaga ttagatagcc
    31681 cagccacccg catagaaggt caccagcgcc acagcgataa cgaccgtacc gatgttcaac
    31741 ttgcgccatt cgccggaaac cagacgacca atcaccagag tcgcgaagcc gatcatgatg
    31801 cctgttacga tgttacaggt cagcacgatg aataccgccg taaccagacc cgccatcgca
    31861 tcaacaaaat cagcaaagtc gattttcgcc acgttactca gcatcagcag gccaacgtac
    31921 atcagcgccg gagccgttgc ataccccgga acgaggtaag agagcggaga gaggaacaga
    31981 atcagcagga acagcacgcc aacggtgata gccgtcaaac cggttttacc gcccgccgcc
    32041 gtacccgccg cagactcgat gtataccgct gccggagctg cacccaccag gccagagaaa
    32101 acgctgctca tggagtcagt ggtcagtgct ttcccgccgt cgatgatctg cccatcttta
    32161 tccagcaggt tcgcctggcc ggcgacggca cggatagttc cggtggcatc aaatactgcc
    32221 gtcatcacca gcgccagaac gcttggcagg actacaggat tcagcgcgcc cataatgtcc
    32281 aggctgccaa ttagtgaatt gccgttttca tcgctcaatg aaggcatggc gaaaacgccg
    32341 gagaaatgga cgttaggatc gaagatcaaa ccgacaattg agataccgat aatggtcagc
    32401 agaatgccac cagggacttt cagtttttcc aggccgataa tcactgccag ccccaccaac
    32461 gacatcatca ccggaaaggt cgtgaaatcc cccagcgcga cgggcagacc atcaagtggg
    32521 tttttaatca ccagaccgac accattagca gcaatcagca gcaggaacag accgatacca
    32581 atccccgtgc cgtgcgccac accgtgcggc aagttgcgca aaatccagct acggataccc
    32641 gtggcagaaa tgaccgtaaa cagcacaccc atcaggaaca cggcacccag cgcgacaggt
    32701 acgctaatat gttgccccag caccagactg aatgcggtaa acgccgtcag ggagatggcg
    32761 caaccaatcg ccaacggcag attagcccac agacccatca cgatagaacc gagtccggca
    32821 accagacagg tcgcaacgaa aactgccgca ggcgggaagc ctgctttacc caacatgcct
    32881 ggaacgacga taaccgagta gaccatcgcc agaaatgttg ttaacccggc aaccacttcc
    32941 tgacggacag tgcttccacg ttgtgaaatt ttaaaccagg cgtcgagtga accgccggta
    33001 cgcgctgatg gcgtagacat aagaaaacat cccctgagag tttaattttc gtcaagatgc
    33061 gtggcggaca atcccctgcc agcgaaacgt tatcttcctg ctccaggttt gcgacaaatt
    33121 ggtgcgtcgc aaaaaagcaa acgtttagct ccgcgcatac aaaacgggtg gtaaaaaaag
    33181 caaacgatta tccggcgatc atagcggctt tttcaaccga agtttgctgc ttttttctct
    33241 tttattgtct tgacgatgaa aaaccaggca attgatgcgc aaacgttatc gtcgatgcgc
    33301 aagattgcag cgttatcgag ccgtataccg ttcaggattg cgataaaaaa tcgtctgaga
    33361 tgacaaaaat attgcggcta aaaagtgtac ttttacactg gcgaagaaac gccggtgaca
    33421 tacaattaaa gcatcaacac caaccggaac ctccaccacg tgctcgaatg aggtgtgttg
    33481 acgtcggggg aaaccctcct gtgtaccagc gggatagaga gaaagacaaa gaccggaaaa
    33541 caaactaaag cgcccttgtg gcgctttagt tttgttcatc ttccagcaag cgtgcgcctg
    33601 tgccttcttc tcctaagcgg tcgcccgggt tacgcaatgg gcaatcactg cgcgaaaggc
    33661 agccacaacc aatacatccg tccagctcgt cacgcagcgc cactaaggta tgaatgcgcc
    33721 gatccaactc ttctcgccat tgggacgaaa gctgtttcca ctctttcgca cttaatgtat
    33781 gcccttcggg caacacgcca aatgcttcac gaatggtcgc cagcggaatg ccaatacgct
    33841 gagcaatttt gatgattgcg acatatcgca acacatcacg tttatatcgc cgctgattgc
    33901 cactattacg gatactgcta atcaaccctt tgctttcata gaaatgcagc gccgataccg
    33961 ccacaccgct gcgttttgcc acttcgccgg gggttagcag cgctttaatg cggggtaatt
    34021 tcttttccat aaatcgcttt acctcaagtt aacttgagga attatactcc ccaacagatg
    34081 aattaacgaa ctgaacactg aaaagaggca gatttatgtc ccatcagaaa attattcagg
    34141 atcttatcgc atggattgac gagcatattg accagccgct taacattgat gtcgtcgcaa
    34201 aaaaatcagg ctattcaaag tggtacttac agcggatgtt ccgtactgtg acgcatcaga
    34261 cactaggtga ttacattcgc caacgccgcc tgttactggc cgccgttgag ttgcgcacca
    34321 ccgagcgtcc gatttttgat atcgcaatgg acctgggtta tgtctctcag cagaccttct
    34381 cccgcgtttt ccgtcggcag tttgatcgca ctcccagcga ttatcgccac cgcctgtagt
    34441 tttattgccc gcgcgttaac tcccgcgcgg gtaattgctc catccattgc ataaacacct
    34501 gcggcggcat cgccttcgca aagaaccatc cctgacaata gcgcacgccg cgtttgcgca
    34561 gccagttaac ctgctcctca gtttcgacgc cttcagcgat cgtttttaac cccagactgt
    34621 gcgccagctc gatgatatgt tccgcaatca aatgactggt tttatgggtg gtcagcgtct
    34681 caacaaacga tttgtcgatt ttcaaaatat cgacattcaa tgatttaagg ttatgcaagt
    34741 tagagtagcc aataccaaaa tcatcaattg ccacttcgta accagcctgg cggaaagcca
    34801 gaataatcgg cgtcattttg tcgacatcaa gaaatgcatg ttcagtcact tcaaatttaa
    34861 tttgctgcgg acgcaccgca tattgctctg ttttctggtt ggttcgcgct atcaaccgtg
    34921 acgtatgaaa atcggaggcc gacaggttaa tagaaacata gcgatcgaca tgtgttgcca
    34981 ggtacacgcc cagatcgcgg aagacattat caataacata atcagttatc tgttcgatca
    35041 tcccctcctt ttctgccagc ggaataaact ctgccggatt cattacttgc ccctgctcac
    35101 caggccaacg taacaatgct tcagctccga tacatttttc tgttttgata tcgattattg
    35161 gctggtaata aaggcaaagt tgatgttttt cgagggcgcg ttgcaatttg cgtttgggag
    35221 acaaatagtt ttgtcgaata cgtagccaga gtaatagcag aacaagactc cccaaaatac
    35281 cggcgggcaa cgcaaaaata agatgattat aaaaatgagt tataagacgt tgatatgaag
    35341 tcgcaacaat ggctgcaatt gggcgttttg tcgaataaac tgtcgcatat aaatagccat
    35401 ttcgttgtac ggttaaatca ttcaaatgaa tcagcggaga aaacgttgct gccgaggcct
    35461 cgttgcttaa cgagaaaaag gttttcgtca ccgtatcata cactccccat tgcaatgtcg
    35521 gatcatcaga catcacttca ctccagaaga gagggttgat aaccgccaca taatttcccc
    35581 gctgcatgta ggtcatttta tagccagaga aaaaaggcgt atcacggtaa taatagatag
    35641 aaacgttagg ttcacgctta taatcggccg gtgcaatcgt atagccattt acagacgcta
    35701 tcagcgatga gcataaaaaa tggttatcac gggcatagat caattcatta atataaagat
    35761 agccacgaat aatattcaac attcgctttt gatgggctgg agtgcaaact tgcccctgat
    35821 agcgttctgc tgcatcgctc actaaatctg cctggcgaat gaccagctcg gatttatcca
    35881 gcgccagctg agcaaaagtt tggagctgac tattcacttc tgattttgcc caaaggaagg
    35941 caatccatag cgacagaatg atgggaaaga gaactaaaaa gataatgccc ggcaacgcca
    36001 gtaattggtg tcgtgcacga tgactcattg ctatcaattt cctgcctgtt tatacccggc
    36061 cattgcaagg tgaaaaccct gggcaccgac gtctttagat aaatcttacg gtataaatag
    36121 cacttttgcc tgatttgaaa ttgatacctt tttaatggtc acctggataa aaataattca
    36181 ttgatattca aaactaattt tctacaggaa tgcagaggcg gcgggaagat atgcccttaa
    36241 tgccactgaa aaaccacata atctgtacag caataaaagt cacggcctaa tctggctaac
    36301 aaacaatcaa ttgtgatata gttcacaaaa ttaatgaaac aaacagagtg tttcattttt
    36361 gtgtcctgca agccccgcga tagccagagt gtaccacgcc tcccgtgaac aacgccgcgc
    36421 tgtccagggc atcggctctt ttcagaggaa ttgtttgatg gctaccctca ccactggcgt
    36481 ggttctactt cgctggcaac ttcttagtgc cgtaatgatg tttctggcca gcacgctcaa
    36541 catccgtttt cgtcggtctg attatgtcgg gcttgcagtg atcagcagcg gtctgggcgt
    36601 ggtttctgcc tgctggttcg caatggggtt gcttggcatc acaatggcgg atatcaccgc
    36661 catctggcac aacatcgagt cggtgatgat tgaagagatg aatcagacac cgccacaatg
    36721 gccaatgatt ttaacttgat atgtagaagc ctccataatg gaggcttctt tttttatggc
    36781 tggcagatgc tttaatcatc caccttaaaa caatataacc tattgtttta atgacaaatc
    36841 agaacggaat atcgtcatca aaatccatcg gcggctcgtt agacggtgct gccggagcgg
    36901 actgctgcgg gcgagactgc gcgccgccgc tgaactgatt gccgccctgc ggctgctgag
    36961 gctgacccca accgccctgc ggctgaccac caccgatatt gccacctgca ggagcgccac
    37021 caccctgacg accacccagc atctgcatgg tgccgccaac gttcaccacg acttctgtgg
    37081 tgtagcgatc ctgaccggat tgatcggtcc atttgcgggt acgcagctga ccttcgatat
    37141 aaacctgaga acctttacgc agatattcgc tggctacttc tgccagtttg ccgaacagca
    37201 caacgcggtg ccactcagtc tgctctttca tctcgccggt cgctttgtca cgccaggatt
    37261 cggaagtagc cagcgtaatg ttggcaacag cgccaccatt tggcatgtag cgtacttccg
    37321 ggtcctgacc cagattacca acgagaataa ccttgtttac gcctctgctg gccatgttcg
    37381 tgtctcctga aaaaaaatcg ttctgaataa gtgtaaacgc gcgattgtac cattaccaat
    37441 agcgctttta ctatgttgtg acctcggttc cgggaaacaa acctggccag acattgttac
    37501 acaacactcc gggtaatgca ttccaatact gtatattcat tcaggtcaat ttgtgtcata
    37561 attaaccgtt tgtgatcgcc ggtagcacca tgccaccggg caaaaaagcg tttaatccgg
    37621 gaaaggtgaa tggataagat cgaagttcgg ggcgcccgca cccataatct caaaaacatc
    37681 aacctcgtta tcccccgcga caagctcatt gtcgtgaccg ggctttcggg ttctggcaaa
    37741 tcctcgctcg ctttcgacac cttatatgcc gaagggcagc gccgttacgt tgaatccctt
    37801 tccgcctacg cgcggcagtt tctgtcactg atggagaagc cggacgtcga ccatattgag
    37861 gggctttctc ctgccatctc aattgagcag aaatcgacgt ctcataaccc gcgctctacg
    37921 gtggggacaa tcaccgaaat ccacgactat ttgcgtctgc tgtacgctcg cgttggtgag
    37981 ccgcgctgcc cggaccacga tgttccgctg gcggcgcaaa ccgtcagcca gatggtggat
    38041 aacgtgcttt cgcagccgga aggcaagcgt ctgatgctgc tcgcgccaat cattaaagag
    38101 cgcaaaggcg aacacaccaa aacgctggaa aatctggcaa gccagggtta catccgtgct
    38161 cgtattgatg gcgaagtctg cgatctttcc gatccgccga aactggaact gcaaaagaaa
    38221 cataccattg aagtggtggt tgatcgcttc aaggtgcgtg acgatctcac ccaacgtctt
    38281 gccgagtcgt ttgaaaccgc gctggagctt tccggtggta ccgcggtagt tgccgatatg
    38341 gacgatccaa aagcggaaga gctgctattt tccgccaact tcgcctgccc aatttgcggc
    38401 tacagtatgc gtgaactgga accacgcctg ttttcgttta acaacccggc gggtgcctgc
    38461 ccgacctgtg acggccttgg cgtacagcaa tatttcgatc ctgaccgcgt gatccagaat
    38521 ccggaactgt cgctggcagg tggtgcgatc cgaggctggg atcgccgcaa cttctattac
    38581 ttccagatgc tgaaatcgct ggcagatcac tataagttcg acgtcgaagc gccgtggggc
    38641 agcctgagcg cgaatgtgca taaagtggtg ttgtacggtt ctggcaaaga aaatattgaa
    38701 ttcaaataca tgaacgatcg tggcgatacc tccattcgtc gtcatccgtt cgaaggcgtg
    38761 ctgcacaata tggagcgccg ctataaagag acggaatcca gtgcggtacg tgaagaatta
    38821 gccaagttta tcagcaatcg tccgtgcgcc agctgcgaag ggacgcgtct gcgtcgggaa
    38881 gcgcgccacg tttatgtcga gaatacgccg ctacctgcta tctccgacat gagcatcggt
    38941 catgcgatgg aattcttcaa caatctcaaa ctcgcaggtc agcgggcgaa gattgcagaa
    39001 aaaatcctta aagagatcgg cgatcgtctg aaattcctcg ttaacgtcgg cctgaattac
    39061 ctgacacttt cccgctcggc agagacactt tctggcggtg aagcccagcg tatccgtctg
    39121 gcgagccaga ttggtgcggg cctggttggc gttatgtacg tgctggacga gccgtctatc
    39181 ggcctgcacc agcgcgataa cgagcgcctg ctgggtacgc ttatccatct gcgcgatctc
    39241 ggtaataccg tgattgtggt ggagcacgac gaagacgcga ttcgcgccgc tgatcatgtg
    39301 atcgatatcg gtccgggtgc gggtgtacac ggcggtgaag tcgtcgcgga aggtccgctg
    39361 gaagcaataa tggcggtgcc agagtcgttg accgggcagt acatgagcgg taaacgcaag
    39421 attgaagtgc cgaagaagcg cgttccggca aatccggaaa aagtgctgaa actgacaggc
    39481 gcacgcggca acaacctgaa agacgtgacg ctgacgctgc cggtgggtct gtttacttgc
    39541 atcacagggg tttcaggttc cggtaaatcg acactgatta acgacacact gttcccgatt
    39601 gcccaacgcc agttgaatgg tgcgaccatc gccgaaccgg caccgtatcg cgatattcag
    39661 ggactggaac atttcgacaa agtgatcgat atcgaccaaa gcccaattgg tcgtacccca
    39721 cgttccaatc cggcaaccta taccggcgtg tttacacctg tacgcgaact gttcgcaggc
    39781 gtaccagaat cccgtgcgcg tggttatacg ccagggcgtt tcagctttaa cgtccgtggc
    39841 ggacgctgcg aggcctgtca gggcgacggc gtgatcaaag tggagatgca cttcctgccg
    39901 gacatttacg taccgtgcga ccagtgcaaa ggcaaacgct ataaccgtga aacgctggag
    39961 attaagtaca aaggcaaaac catccacgaa gtgctggata tgaccatcga agaggcgcgt
    40021 gagttctttg atgcggtgcc agctctggca cgtaagctgc aaacgctgat ggatgttggc
    40081 ctgacgtaca ttcgcctcgg gcagtccgca accacacttt ctggtggtga agcccagcgc
    40141 gtgaagctgg cgcgtgagct gtcaaaacgc ggcaccgggc agacgctgta tattctcgac
    40201 gagccgacca ccggtctgca cttcgccgat attcagcaac tgctcgacgt actgcataaa
    40261 ctgcgcgatc agggcaatac cattgtggta attgagcaca atctcgacgt gatcaaaacc
    40321 gctgactgga ttgtcgacct gggaccggaa ggcggcagtg gcggcggcga aatcctcgtc
    40381 tccggtacgc cagaaaccgt cgcagagtgc gaagcttcgc atacggcgcg cttcctcaag
    40441 ccgatgctgt aatcgttaag gccgctttca gagcggcctt cttctttcag agttgcacca
    40501 gcaatttacg tttttcttcc ggcagcaaat tcaccgcctg ctgataagac gcatccacca
    40561 gataatagat ttgcgaatcc ggcagcgaac cgtcgagata cacggtgctc cagtgcgctt
    40621 tattcagatg gcggcttgga cgcacatcgc tgtgctgctg acgtagcagc tccgccagct
    40681 ccgggctggt tttcagcgaa acagctgggc gattttctac ttctttcacc atcgcaaaca
    40741 gcacatcttc cactttgatc tgcgtcgctt tccagtcgtt atgcacgctc tgttctgcgc
    40801 ctggttttgc catgcaatat tgtagcaact ccgaaatggt catttttcac tccccttgta
    40861 gtgtcgcgat gatgcgacgc gatccgccgt ggatgcgatg ttcccccagc cagatgcctt
    40921 gccaggtgcc ggtctgaata cgccctttat gcaccggcaa tacaagcgat gttcccagca
    40981 ttgaggattt gatatgagaa ggcatatcgt ctgctccctc atagtcatgc tcataatttc
    41041 cgttatcggg aacggcgcgg aggaaaaaac gctccatgtc gtggcgtacg gtgggatcgc
    41101 agttctcatt aagtgtcaga gaggcggagg tatgttgcag caacagatgc agtaaaccga
    41161 tgttaacgcg cggcatatca gccagctgat tcagaatttc atccgttacc agatgaaacc
    41221 cacgagattt ggcgctaagc gtgagcgtct tttgatacca catgtgctgc tccttgataa
    41281 aactctctta atcagtttgc agcaagacag cgaaaggata aaggtgtgat taaaaaaaca
    41341 gcattcggga gagtattacg ctctcccgct ctgtcagtat tctgaattga cgatcacctc
    41401 ttcaccaaac gcacccgctt gtggcaaggg tttgtaggta gagttggagg cgcgcagaat
    41461 gcggatacca cgagcgccga catcgcgtgc ggcggtaata tcgttatcgg aatcaccata
    41521 aaaaattcgg atatttttat cctgcagcca ttgcgatttt gtattttgcc ctggtttatc
    41581 gcccgcaaag atcaccggat tcatgttggt ggcaggaata tgaaaattat ccgccagcgt
    41641 ttttgaaacc gtttctgttt tggtcgggct acgaccagtc acaaagaaga tcgcgtcacc
    41701 gcggcgtaca tgcatatcaa tcagctggcg agcgacctct tttggaatgc tgaattcatc
    41761 ccagccattg ttcatttttt cccagaacac aggatttttc agataatctt cgctttctgg
    41821 cgagaaggtt tttttgccgc gccagaagcc cggactggaa aaaagtaccg tgtcatcgat
    41881 atcaaacccc accgccattg gcggacgccc tgcgaggcta ttttcaattt gtgcgaccga
    41941 aacccaatga attggtgcct gttcagcaag cctggcaacg ttagtaccag ggttaagcgg
    42001 tgaaggagat gaggccaggg caacagcgga actgtttaga gcgaacaata agcaaacggc
    42061 actgattgcc tgtgtgatct tgcgcatatt tttccctaaa tagtcagtat gttgaaactt
    42121 tttattgtga gatttgttgc aaaaaccatc tgaccataac gccagcagcc ggggaaagga
    42181 aggattatct gcggt
//
LOCUS       DAZFLD010000012        41010 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.012, whole genome shotgun sequence.
ACCESSION   DAZFLD010000012 DAZFLD010000000
VERSION     DAZFLD010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 41010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41010
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.012"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            94..1092
                     /locus_tag="ACHKBL_000593"
     CDS             94..1092
                     /locus_tag="ACHKBL_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HFO5817515.1"
                     /translation="MKLFRILDPFTLTLITVVLLASFFPARGDFVPFFENLTTAAIAL
                     LFFMHGAKLSREAIIAGGGHWRLHLWVMCSTFVLFPILGVLFAWWKPVNVDPMLYSGF
                     LYLCILPATVQSAIAFTSMAGGNVAAAVCSASASSLLGIFLSPLLVGLVMNVHGAGGS
                     LEQVGKIMLQLLLPFVLGHLSRPWIGDWVSRNKKWIAKTDQTSILLVVYTAFSEAVVN
                     GIWHKVGWGSLLFIVVVSCVLLAIVIVVNVFMARRLGFNKADEITIVFCGSKKSLANG
                     IPMANILFPTSVIGMMVLPLMIFHQIQLMVCAVLARRYKRQTEQLQAQQESSADKA"
     gene            complement(1089..1307)
                     /locus_tag="ACHKBL_000594"
     CDS             complement(1089..1307)
                     /locus_tag="ACHKBL_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HFO5817516.1"
                     /translation="MEKEQLIEIANTIMPFGKYKGRRLIDLPEEYLLWFARKDEFPAG
                     KLGELMQITLLIKTEGLTQLVQPLKRPL"
     gene            complement(1309..3324)
                     /gene="ligA"
                     /locus_tag="ACHKBL_000595"
     CDS             complement(1309..3324)
                     /gene="ligA"
                     /locus_tag="ACHKBL_000595"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HFO5817517.1"
                     /translation="MESIEQQLTELRTTLRHHEYLYHVMDAPEIPDAEYDRLMRELRE
                     LETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRL
                     KSNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH
                     GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKR
                     PLTFFCYGVGVLEGGELPDTHLGRLMQFKAWGLPVSDRVTLCESAEEVLAFYHEVEKD
                     RPTLGFDIDGVVIKVNSLAQQEQLGFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRT
                     GAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVL
                     SERPEDTREVVFPTHCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMD
                     VDGMGDKIIDQLVEKEYVHTPADLFKLTAVKLTGLERMGPKSAQNVVNALEKAKETTF
                     ARFLYALGIREVGEATAAGLAAYFGTLEALEAASIEELQKVPDVGIVVASHVHNFFAE
                     ESNRNVISELLAEGVHWPAPVVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVEL
                     GAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIEVIDETEMLRLLGS"
     gene            complement(3395..4393)
                     /gene="zipA"
                     /locus_tag="ACHKBL_000596"
     CDS             complement(3395..4393)
                     /gene="zipA"
                     /locus_tag="ACHKBL_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HFO5817518.1"
                     /translation="MMQDLRLILIIVGAIAIIALLVHGFWTSRKERSSMFRDRPLKRM
                     KSKRDDDSYDEDVEDDEGVGEVRVHRVNHAPANAQEHEAARPSPQHQYQPPYASAQPR
                     QPVQQPPEAQVPPQHAPRPAQLVQQPVQQPAYQPQPEQPLQQPVSPQVAPAPQPVHSA
                     PQPAQQAFQPAEPVAAPQPEPVAEPAPVMDKPKRKEAVIIMNVAAHHGSELNGELLLN
                     SIQQAGFIFGDMNIYHRHLSPDGSGPALFSLANMVKPGTFDPEMKDFTTPGVTIFMQV
                     PSYGDELQNFKLMLQSAQHIADEVGGVVLDDQRRMMTPQKLREYQDIIREVKDANA"
     gene            4623..5384
                     /gene="cysZ"
                     /locus_tag="ACHKBL_000597"
     CDS             4623..5384
                     /gene="cysZ"
                     /locus_tag="ACHKBL_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HFO5817519.1"
                     /translation="MVSSFTSAPRSGFYYFAQGWKLVSQPGIRRFVILPLLVNILLMG
                     GAFWWLFTQLDVWIPTLMSYVPDWLQWLSYLLWPLAVISVLLVFGYFFSTIANWIAAP
                     FNGLLAEQLEARLTGATPPDTGIFGIMKDVPRIMKREWQKFAWYLPRAIVLLILYFIP
                     GIGQTVAPVLWFLFSAWMLAIQYCDYPFDNHKVPFKEMRTALRTRKITNMQFGALTSL
                     FTMIPLLNLFIMPVAVCGATAMWVDCYRDKHAMWR"
     gene            5569..6540
                     /gene="cysK"
                     /locus_tag="ACHKBL_000598"
     CDS             5569..6540
                     /gene="cysK"
                     /locus_tag="ACHKBL_000598"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HFO5817520.1"
                     /translation="MSKIFEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCR
                     IGANMIWDAEKRGVLKPGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRK
                     LLKALGANLVLTEGAKGMKGAIQKAEEIVASNPEKYLLLQQFSNPANPEIHEKTTGPE
                     IWEDTDGQVDVFIAGVGTGGTLTGVSRYIKGTKGKTDLISVAVEPTDSPVIAQALAGE
                     EIKPGPHKIQGIGAGFIPANLDLKLVDKVIGITNEEAISTARRLMEEEGILAGISSGA
                     AVAAALKLQEDESFTNKNIVVILPSSGERYLSTALFADLFTEKELQQ"
     gene            6924..7181
                     /gene="ptsH"
                     /locus_tag="ACHKBL_000599"
     CDS             6924..7181
                     /gene="ptsH"
                     /locus_tag="ACHKBL_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HFO5817521.1"
                     /translation="MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASA
                     KSLFKLQTLGLTQGTVVTISAEGEDEQKAVEHLVKLMAELE"
     gene            7226..8953
                     /gene="ptsI"
                     /locus_tag="ACHKBL_000600"
     CDS             7226..8953
                     /gene="ptsI"
                     /locus_tag="ACHKBL_000600"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HFO5817522.1"
                     /translation="MISGILASPGIAFGKALLLKEDEIVIDRKKISADQVDQEVERFL
                     SGRAKASAQLETIKTKAGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADA
                     AAHEVIEGQASALEELDDEYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVA
                     TDLTPSETAQLNLKKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYL
                     ILDAVNNQVYVNPTNEVIDKMRAVQEQVASEKAELAKLKDLPAITLDGHQVEVCANIG
                     TVRDVEGAERNGAEGVGLYRTEFLFMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTM
                     DIGGDKELPYMNFPKEENPFLGWRAIRIAMDRKEILRDQLRAILRASAFGKLRIMFPM
                     IISVEEVRALRKEIEIYKQELRDEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFS
                     IGTNDLTQYTLAVDRGNDMISHLYQPMSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     ERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDELMTLVN
                     KFIEEKTIC"
     gene            8994..9503
                     /gene="crr"
                     /locus_tag="ACHKBL_000601"
     CDS             8994..9503
                     /gene="crr"
                     /locus_tag="ACHKBL_000601"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFO5817523.1"
                     /translation="MGLFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAE
                     KIVGDGIAIKPTGNKMVAPVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGE
                     GFKRIAEEGQRVKVGDTVIEFDLPLLEEKAKSTLTPVVISNMDEIKELIKLSGSVTVG
                     ETPVIRIKK"
     gene            complement(9545..10396)
                     /gene="pdxK"
                     /locus_tag="ACHKBL_000602"
                     /gene_synonym="thiJ"
     CDS             complement(9545..10396)
                     /gene="pdxK"
                     /locus_tag="ACHKBL_000602"
                     /gene_synonym="thiJ"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine/pyridoxal/pyridoxamine kinase"
                     /protein_id="HFO5817524.1"
                     /translation="MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGL
                     NVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQ
                     IKILAEWLTALRKDHPDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNI
                     FELEILTGKNCRDLDSAIAAAKSLLSDTLKWVVITSASGNEENQEMQVVVVSADSVNV
                     ISHSRVKTDLKGTGDLFCAQLISGLLKGKALNDAVHRAGLRVLEVMRYTQQHESDELI
                     LPPLAEA"
     gene            10501..10869
                     /locus_tag="ACHKBL_000603"
     CDS             10501..10869
                     /locus_tag="ACHKBL_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001592994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeK family protein"
                     /protein_id="HFO5817525.1"
                     /translation="MKKIICLVITLLMTLPVYAKLTAHEEARINAMLEGLAQKKDLIF
                     VRNGDDHTCDEAVSHLRLKLGNTRNRIDTAEQFIDKVASSSSITGKPYIVKMPGKSDE
                     NAQPFLHALIAQTDKTVPAQ"
     gene            complement(10872..11783)
                     /gene="cysM"
                     /locus_tag="ACHKBL_000604"
     CDS             complement(10872..11783)
                     /gene="cysM"
                     /locus_tag="ACHKBL_000604"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase B"
                     /protein_id="HFO5817526.1"
                     /translation="MSTLEQTIGNTPLVKLQRMAPDNGSEVWLKLEGNNPAGSVKDRA
                     ALSMIVEAEKRGEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAA
                     MLAYGAELILVTKEQGMEGARDLALEMANRGEGKLLDQFNNPDNPFAHYTTTGPEIWQ
                     QTGGRITHFVSSMGTTGTITGVSRFMREQSKPVTIVGLQPEEGSSIPGIRRWPAEYLP
                     GIFNASLVDEVLDIHQRDAENTMRELAVREGIFCGVSSGGAVAGALRVAKANPGAVVV
                     AIICDRGDRYLSTGVFGEEHFSQGAGI"
     gene            complement(11917..13014)
                     /gene="cysA"
                     /locus_tag="ACHKBL_000605"
     CDS             complement(11917..13014)
                     /gene="cysA"
                     /locus_tag="ACHKBL_000605"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter ATP-binding
                     protein CysA"
                     /protein_id="HFO5817527.1"
                     /translation="MSIEIANIKKSFGRTQVLNDISLDIPSGQMVALLGPSGSGKTTL
                     LRIIAGLEHQTSGHIRFHGTDVSRLHARDRKVGFVFQHYALFRHMTVFDNIAFGLTVL
                     PRRERPNAAAIKAKVTKLLEMVQLAHLADRYPAQLSGGQKQRVALARALAVEPQILLL
                     DEPFGALDAQVRKELRRWLRQLHEELKFTSVFVTHDQEEATEVADRVVVMSQGNIEQA
                     DAPDQVWREPATRFVLEFMGEVNRLQGTIRGGQFHVGAHRWPLGYTPAYQGPVDLFLR
                     PWEVDISRRTSLDSPLPVQVLEASPKGHYTQLVVQPLGWYNEPLTVVMHGDDAPQRGE
                     RLFVGLQHARLYNGDERIETRDEELALAQSA"
     gene            complement(13004..13879)
                     /gene="cysW"
                     /locus_tag="ACHKBL_000606"
     CDS             complement(13004..13879)
                     /gene="cysW"
                     /locus_tag="ACHKBL_000606"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HFO5817528.1"
                     /translation="MAEVTQLKRYDARPINWGKWFLIGIGMLVSAFILLVPMIYIFVQ
                     AFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVNLVFGILLAWLVTRFNFPGRQLL
                     LTLLDIPFAVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPF
                     VVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEF
                     GAVSVVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRL
                     ENQEKRAQQEEHHEH"
     gene            complement(13879..14712)
                     /gene="cysT"
                     /locus_tag="ACHKBL_000607"
     CDS             complement(13879..14712)
                     /gene="cysT"
                     /locus_tag="ACHKBL_000607"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysT"
                     /protein_id="HFO5817529.1"
                     /translation="MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLAQMSW
                     AQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDL
                     PFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQ
                     PVLEELGPEYEEAAETLGATRWQSFRKVVLPELSPALVAGVALSFTRSLGEFGAVIFI
                     AGNIAWKTEVTSLMIFVRLQEFDYPAASAIASVILAASLLLLFSINTLQSRFGRRVVG
                     H"
     gene            complement(14712..15728)
                     /gene="cysP"
                     /locus_tag="ACHKBL_000608"
     CDS             complement(14712..15728)
                     /gene="cysP"
                     /locus_tag="ACHKBL_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate/sulfate ABC transporter
                     substrate-binding protein CysP"
                     /protein_id="HFO5817530.1"
                     /translation="MAVNLLKKNSLVLVASLLLAGHVQATELLNSSYDVSRELFAALN
                     PPFEQQWAKDNGGDKLTIKQSHAGSSKQALAILQGLKADVVTYNQVTDVQILHDKGKL
                     IPADWQSRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
                     TYLAAWGAADKADGGDKAKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFE
                     SEVNNIRKQYEAQGFEVVIPKTNILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQA
                     QTIITDYYYRVNNPEVMGKLKDKFPQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLA
                     AGRK"
     gene            complement(15886..16677)
                     /gene="ucpA"
                     /locus_tag="ACHKBL_000609"
     CDS             complement(15886..16677)
                     /gene="ucpA"
                     /locus_tag="ACHKBL_000609"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase UcpA"
                     /protein_id="HFO5817531.1"
                     /translation="MGKLTGKTALITGALQGIGEGIARTFARHGANLILLDISPEIEK
                     LADELCGRGHRCTAVVADVRDPASVAAAIKRAKEKEGRIDILVNNAGVCRLGSFLDMS
                     DEDRDFHIDINIKGVWNVTKAVLPEMIARKDGRIVMMSSVTGDMVADPGETAYALTKA
                     AIVGLTKSLAVEYAQSGIRVNAICPGYVRTPMAESIARQSNPEDPESVLTEMAKAIPM
                     RRLADPLEVGELAAFLASDESSYLTGTQNVIDGGSTLPETVSVGI"
     gene            complement(16806..17663)
                     /gene="murR"
                     /locus_tag="ACHKBL_000610"
     CDS             complement(16806..17663)
                     /gene="murR"
                     /locus_tag="ACHKBL_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MurR"
                     /protein_id="HFO5817532.1"
                     /translation="MLYLTKIRNAESEFTGNEQKIADFLRANVSELKSVSSRKMAKQL
                     GISQSSIVKFAQKLGAQGFTELRMALIGEYSASREKTNATALHLHSSITSDDSLEVIA
                     RKLNREKEQALEQTCALFDYARLQKIIEVISKAPFIQITGLGGSELVGRDLSFKLMKI
                     GYRVACEADTHVQATVSQALKKGDVQIAISYSGSKKEIVLCAEAARKQGATVIAITSL
                     TDSPLRRLAHYTLDTVSGETEWRSSSMSTRTAQNSVTDLLFVGLVQLNDVESLKMIER
                     SSELTQRLK"
     gene            17827..18723
                     /gene="murQ"
                     /locus_tag="ACHKBL_000611"
     CDS             17827..18723
                     /gene="murQ"
                     /locus_tag="ACHKBL_000611"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFO5817533.1"
                     /translation="MQLEKMITEGSNAASAEIDRVSTLEMCRIINDEDKTVPLAVERV
                     LPDIAAAIDVIHAQVSGGGRLIYLGAGTSGRLGILDASECPPTYGVKPGLVVGLIAGG
                     EYAIQHAVEGAEDSQEGGVNDLKNIGLTAQDVVVGIAASGRTPYVIAGLEYARQLGCR
                     TVGISCNPGSAVSTTAEFAITPIVGPEVVTGSSRMKAGTAQKLVLNMLSTGLMIKSGK
                     VFGNLMVDVVATNEKLHVRQVNIVKNATGCSAEQAEAALIACERNCKTAIVMVLKNLD
                     AAEAKKRLDQHGGFIRQVLDKE"
     gene            18727..20151
                     /gene="murP"
                     /locus_tag="ACHKBL_000612"
     CDS             18727..20151
                     /gene="murP"
                     /locus_tag="ACHKBL_000612"
                     /EC_number="2.7.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylmuramic acid transporter subunit
                     IIBC"
                     /protein_id="HFO5817534.1"
                     /translation="MAKEISSELLNTILTRVGGPGNIASCGNCMTRLRLGVHDSSLVD
                     PNIKTLEGVKGVILTSDQVQVVFGPGKAHRAAKAMSELLGEAPVQDAAEIAAQNKRQL
                     KAKQTSGVQQFLAKFATIFTPLIPGFIAAGLLLGIATLIATVMHVPADAQGTLPDALN
                     FMKVFSKGLFTFLVILVGYNAAQAFGGTGVNGAIIAALFLLGYNPTATTGYYAGFHDF
                     FGLPIDPRGNIIGVLIAAWACARIEGMVRRFMPDDLDMLLTSLITLLITATLAYLIIM
                     PLGGWLFEGMSWLFMHLNSNPLGCAVLAGLFLIAVVFGVHQGFIPVYLALMDSQGFNS
                     LFPILSMAGAGQVGAALALYWRAQPHSALRSQVRGAIIPGLLGVGEPLIYGVTLPRMK
                     PFVTACLGGAAGGLFIGLIAWWGLPMGLNSAFGPSGLVALPLMTSAQGILPAMAVYAG
                     GILVAWVCGFIFTTLFGCRNVNLD"
     gene            20156..21457
                     /gene="pbp4b"
                     /locus_tag="ACHKBL_000613"
     CDS             20156..21457
                     /gene="pbp4b"
                     /locus_tag="ACHKBL_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015674721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin binding protein PBP4B"
                     /protein_id="HFO5817535.1"
                     /translation="MKRTMLYLSLLAVSCSVSAAKYPVLTESSPEKAGFNVERLNQMD
                     RWISQQVDAGYPGVNLLIIKDNQIVYRKAWGAAKKYDGSALMAQPVKATTGTLYDLAS
                     NTKMYATNFALQKLMSEGKLHPDDRIAKYIPGFADSPNDAIKGKNTLRISDLLHHSGG
                     FPADPQYPNKAVAGALYSQDKGQTLEMIKRTPLEYQPCSKHIYSDVDYMLLGFIVESV
                     TGQPLDRYVEESIYRPLGLTHTVFNPLLKGFKPQQIAATELNGNTRDGVIHFPNIRTS
                     TLWGQVHDEKAFYSMGGVSGHAGLFSDTGDIAVLMQTMLNGGGYGDVQLFNAETVKMF
                     TTSSKEDATFGLGWRVNGNATMTPTFGTLASPQTYGHTGWTGTVTVIDPVNHMAIVML
                     SNKPHSPVADPQKNPNMFESGQLPIATYGWVVDQVYAALKQ"
     gene            complement(21515..22414)
                     /gene="yfeX"
                     /locus_tag="ACHKBL_000614"
     CDS             complement(21515..22414)
                     /gene="yfeX"
                     /locus_tag="ACHKBL_000614"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311329.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphyrinogen peroxidase"
                     /protein_id="HFO5817536.1"
                     /translation="MSQVQSGILPEHCRAAIWIEANVKGEVDVLRAASKTFADKLATF
                     EAKFPDAHLGAVVAFGNNTWRALSGGVGAEELKDFPGYGKGLAPTTQFDVLIHILSLR
                     HDVNFSVAQAAMEAFGDCIEVKEEIHGFRWVEERDLSGFVDGTENPAGEETRREVAVI
                     KDGVDAGGSYVFVQRWEHNLKQLNRMSIHDQEMMIGRTKEANEEIDGDERPETSHLTR
                     VDLKEDGKGLKIVRQSLPYGTASGTHGLYFCAYCARLHNIEQQLLSMFGDTDGKRDAM
                     LRFTKPVTGGYYFAPSLDKLMAL"
     gene            complement(22510..23085)
                     /locus_tag="ACHKBL_000615"
     CDS             complement(22510..23085)
                     /locus_tag="ACHKBL_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoE-regulated lipoprotein"
                     /protein_id="HFO5817537.1"
                     /translation="MKSLRLMLCAMPLMLTGCSTMSSVNWSAANPWNWFGSSTKVSEQ
                     GVGELTASTPLQEQAIADALDGDYRLRSGMKTANGNVVRFFEVMKGDNVAMVINGDQG
                     TISRIDVLDSDIPADTGVKIGTPFSDLYSKAFGNCQKADGDDNRAVECKAEGSQHISY
                     QFSGEWSGPEGLMPSDDTLKNWKVSKIIWRR"
     gene            complement(23146..23595)
                     /locus_tag="ACHKBL_000616"
     CDS             complement(23146..23595)
                     /locus_tag="ACHKBL_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2919 family protein"
                     /protein_id="HFO5817538.1"
                     /translation="MKSTEFHPVHYDAYGRLRLPLLFWLVLLLQARTWVLFVIAGASR
                     EQGTALLNLFYPDHDNFWLGLIPGIPAVLAFLLSGRRASFPRIWHVLYFLLLLAQVVL
                     LCWQPWLWLNGESVSGIGLALVVADIVALIWLLTNRRLRACFNEEKE"
     gene            complement(23582..24007)
                     /locus_tag="ACHKBL_000617"
     CDS             complement(23582..24007)
                     /locus_tag="ACHKBL_000617"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HFO5817539.1"
                     /translation="MEIRVFRQEDFEEVITLWERCDLLRPWNDPEMDIERKMNHDVSL
                     FLVAEVNGEVVGTVMGGYDGHRGSAYYLGVHPEFRGRGIANALLNRLEKKLIARGCPK
                     IQINVPEDNDMVLGMYERLGYEHADVLSLGKRLIEDEEY"
     gene            24221..25090
                     /gene="amiA"
                     /locus_tag="ACHKBL_000618"
     CDS             24221..25090
                     /gene="amiA"
                     /locus_tag="ACHKBL_000618"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiA"
                     /protein_id="HFO5817540.1"
                     /translation="MSTFKPLKTLTSRRQVLKAGLAALTLSGMSQAIAKEEPLKTSNG
                     HSKPKAKKSGGKRVVVLDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDA
                     RLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMA
                     KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKL
                     HSRNTEQAAFVVLKSPSVPSVLVETSFITNPEEERLLGTAAFRQKIATAIAEGVISYF
                     HWFDNQKAHSRKR"
     gene            25094..25993
                     /gene="hemF"
                     /locus_tag="ACHKBL_000619"
     CDS             25094..25993
                     /gene="hemF"
                     /locus_tag="ACHKBL_000619"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HFO5817541.1"
                     /translation="MKPDAHQVKQFLLNLQDTICQQLSAVDGTEFVEDSWQREAGGGG
                     RSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYV
                     PTSHANVRFFIAEKPGAEPVWWFGGGFDLTPFYGFEDDAIHWHRTARDLCLPFGEDVY
                     PRYKKWCDDYFYLKHRNEQRGIGGLFFDDLNTPDFDHCFAFMQAVGKGYTDAYLPIVE
                     RRKTMAYGERERNFQLYRRGRYVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEY
                     DYQPEDGSPEAALSEFIKVRDWV"
     gene            complement(25927..27051)
                     /gene="eutR"
                     /locus_tag="ACHKBL_000620"
     CDS             complement(25927..27051)
                     /gene="eutR"
                     /locus_tag="ACHKBL_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001735543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator EutR"
                     /protein_id="HFO5817542.1"
                     /translation="MKKTRTANLHHLYHEPLPENLKLTPKVEVDNVHQRQTTDVYEHA
                     LTITAWQQIYDQLHPGKFHGEFTEILLDDIQVFREYTGLALRQSCLVWPNSFWFGIPA
                     TRGEQGFIGSQCLGSAEIATRPGGTEFELSTPDDYTILGVVLSEDVITRQANFLHNPD
                     RVLHMLRSQSALEVKEQHKAALWGFVQQALATFCENPENLHQPAVRKVLGDNLLMAMG
                     AMLEEAQPMVTAESISHQSYRRLLSRAREYVLENMSEPVTVLDLCNQLHVSRRTLQNA
                     FHAILGIGPNAWLKRIRLNAVRRELISPWSQSTTVKDAAMQWGFWHLGQFATDYQQLF
                     AEKPSLTLHQRMREWGGGSYTQSLTLINSLNAASGLPSSG"
     gene            complement(27097..27597)
                     /gene="eutK"
                     /locus_tag="ACHKBL_000621"
     CDS             complement(27097..27597)
                     /gene="eutK"
                     /locus_tag="ACHKBL_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311336.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutK"
                     /protein_id="HFO5817543.1"
                     /translation="MINALGLLEVDGMVAAIDAADAMLKAANVRLLSHEVLDPGRLTL
                     VVEGDLAACRAALDAGCAAAMRTGRVISRKEIGRPDDDTQWLVTGFNRQPKQPVKEPD
                     APVIVAESADELLALLTSVRQGMTAGEVAAHFGWPLEKARNALEQLFSAGTLRKRSSR
                     YRLKPH"
     gene            complement(27610..28269)
                     /gene="eutL"
                     /locus_tag="ACHKBL_000622"
     CDS             complement(27610..28269)
                     /gene="eutL"
                     /locus_tag="ACHKBL_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HFO5817544.1"
                     /translation="MPALDLIRPSVTAMRVIASVNAEFARELKLPPHIRSLGLISADS
                     DDVTYIAADEATKQAMVEVVYGRSLYAGAAHGPSPTAGEVLIMLGGPNPAEVRAGLDA
                     MVAHIENGAAFQWANDAENTAFLAHVVSRTGSYLSSTAGITLGDPMAYLVAPPLEATY
                     GIDAALKSADVQLVTYVPPPSETNYSAAFLTGSQAACKAACNAFTDAVLEIARNPIQR
                     A"
     gene            complement(28279..29166)
                     /gene="eutC"
                     /locus_tag="ACHKBL_000623"
     CDS             complement(28279..29166)
                     /gene="eutC"
                     /locus_tag="ACHKBL_000623"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit beta"
                     /protein_id="HFO5817545.1"
                     /translation="MDQKQIEEIVRSVMASMGQAAPAPSEAKCATTTCTTPVTSESCA
                     LDLGSAEAKAWIGVENPHRADVLTELRRSTVARVCTGRAGPRPRTQALLRFLADHSRS
                     KDTVLKEVPEEWVKAQGLLEVRSEISDKNLYLTRPDMGRRLCAEAVEALKAQCVANPD
                     VQVVISDGLSTDAITVNYEEILPPLMAGLKQAGLKVGTPFFVRYGRVKIEDQIGEILG
                     AKVVILLVGERPGLGQSESLSCYAVYSPRMATTVEADRTCISNIHQGGTPPVEAAAVI
                     VDLAKRMLEQKASGINMTR"
     gene            complement(29187..30548)
                     /gene="eutB"
                     /locus_tag="ACHKBL_000624"
     CDS             complement(29187..30548)
                     /gene="eutB"
                     /locus_tag="ACHKBL_000624"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit alpha"
                     /protein_id="HFO5817546.1"
                     /translation="MKLKTTLFGNVYQFKDVKEVLAKANELRSGDVLAGVAAASSQER
                     VAAKQVLSEMTVADIRNNPVIAYEDDCVTRLIQDDVNETAYNQIKNWSISELREYVLS
                     DETSVDDIAFTRKGLTSEVVAAVAKICSNADLIYGAKKMPVIKKANTTIGIPGTFSAR
                     LQPNDTRDDVQSIAAQIYEGLSFGVGDAVIGVNPVTDDVENLSRVLDTIYGVIDKFNI
                     PTQGCVLAHVTTQIEAIRRGAPGGLIFQSICGSEKGLKEFGVELAMLDEARAVGAEFN
                     RIAGENCLYFETGQGSALSAGANFGADQVTMEARNYGLARHYDPFIVNTVVGFIGPEY
                     LYNDRQIIRAGLEDHFMGKLSGISMGCDCCYTNHADADQNLNENLMILLATAGCNYIM
                     GMPLGDDIMLNYQTTAFHDTATVRQLLNLRPSPEFERWLESMGIMANGRLTKRAGDPS
                     LFF"
     gene            complement(30560..31963)
                     /gene="eutA"
                     /locus_tag="ACHKBL_000625"
     CDS             complement(30560..31963)
                     /gene="eutA"
                     /locus_tag="ACHKBL_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HFO5817547.1"
                     /translation="MNTRQLLSVGIDIGTTTTQVIFSHLELVNRAAVSQVPRYEFIKR
                     EISWQSPVFFTPVDKQGGLKEAELKTLILEQYQAAGIAPESVDSGAIIITGESAKTRN
                     ARPAVMTLSQSLGDFVVASAGPHLESVIAGHGAGAQTLSEQRLCRVLNIDIGGGTANY
                     ALFDAGKISGTACLNVGGRLLETDSQGRVVYAHKPGQMIVDECFGAGTEARSLTGAQL
                     VQVTRRMAELIVEVIDGTLSPLAQALMQTGLLPVGVTPEIITLSGGVGECYRHQPADP
                     FCFADIGPLLATALHDHPRLREMNVQFPAQTVRATVIGAGAHTLSLSGSTIWLEGVQL
                     PLRNLPVAIPIDEKDLVSAWQQALIQLDLDPKTDAYVLALPASLPVRYAAVLTVINAL
                     VDFVARFPNPHPLLVVAGQDFGKALGMLLRPQLQQLPLAVIDEVIVRAGDYIDIGTPL
                     FGGSVVPVTVKSLAFPS"
     gene            complement(31960..33186)
                     /gene="eutH"
                     /locus_tag="ACHKBL_000626"
     CDS             complement(31960..33186)
                     /gene="eutH"
                     /locus_tag="ACHKBL_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0034228 - ethanolamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034229 - ethanolamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HFO5817548.1"
                     /translation="MGINEIIMYIMMFFMLIAAVDRILSQFGGSARFLGKFGKSIEGS
                     GGQFEEGFMAMGALGLAMVGMTALAPVLAHVLGPVIIPVYEMLGANPSMFAGTLLACD
                     MGGFFLAKELAGGDVAAWLYSGLILGSMMGPTIVFSIPVALGIIEPSDRRYLALGVLA
                     GIVTIPIGCIAGGLVAMYSGVQINGQPVEFTFALILMNMIPVLIVAVLVALGLKFIPE
                     KMINGFQIFAKFLVALITLGLAAAVVKFLLGWELIPGLDPIFMAPGDKPGEVMRAIEV
                     IGSISCVLLGAYPMVLLLTRWFEKPLMSVGKVLNMNNIAAAGMVATLANNIPMFGMMK
                     QMDTRGKVINCAFAVSAAFALGDHLGFAAANMNAMIFPMIVGKLIGGVTAIGVAMMLV
                     PKEDASAAKTEAEAQS"
     gene            complement(33403..34590)
                     /gene="eutG"
                     /locus_tag="ACHKBL_000627"
     CDS             complement(33403..34590)
                     /gene="eutG"
                     /locus_tag="ACHKBL_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization ethanol dehydrogenase
                     EutG"
                     /protein_id="HFO5817549.1"
                     /translation="MQSELQTALFQAFDTLNLQRVKTFSVPPVTLCGPGAVSSCGQQA
                     QTRGLKHLFVMADSFLHQAGMTAGLTRSLAVKGIAMTLWPCPVGEPCITDVCAAVAQL
                     RESGCDGVIAFGGGSVLDAAKAVALLVTNPDSTLAEMSETSVLQPRLPLIAIPTTAGT
                     GSETTNVTVIIDAVSGRKQVLAHASLMPDVAILDAALTEGVPSHVTAMTGIDALTHAI
                     EAYSALNATPFTDSLAIGAIAMIGKSLPKAVGYGHDLAARESMLLASCMAGMAFSSAG
                     LGLCHAMAHQPGAALHIPHGLANAMLLPTVMEFNRMVCRDRFSLVGRALRTKKSDDRD
                     AINAVSELIAEVGIGKRLGDVGATSAHYGAWAQAALEDICLRSNPRTASLEQIVGLYA
                     AAQ"
     gene            complement(34580..35416)
                     /gene="eutJ"
                     /locus_tag="ACHKBL_000628"
     CDS             complement(34580..35416)
                     /gene="eutJ"
                     /locus_tag="ACHKBL_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutJ"
                     /protein_id="HFO5817550.1"
                     /translation="MAHDEQWLTPRLQTAATLCNQTPAATESPLWLGVDLGTCDVVSM
                     VVDRDGQPVAVCLDWADVVRDGIVWDFFGAVTIVRRHLDTLEQQFGRRFSHAATSFPP
                     GTDPRISINVLESAGLEVSHVLDEPTAVADLLQLDNAGVVDIGGGTTGIAIVKKGKVT
                     YSADEATGGHHISLTLAGNRRISLEEAEQYKRGHGDEIWPAVKPVYEKMADIVARHIE
                     GQGITDLWLAGGSCMQPGVAELFRKQFPALQVHLPQHSLFMTPLAIASSGREKTEGIY
                     AK"
     gene            complement(35427..36830)
                     /locus_tag="ACHKBL_000629"
     CDS             complement(35427..36830)
                     /locus_tag="ACHKBL_000629"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004029 - aldehyde dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0008774 - acetaldehyde dehydrogenase
                     (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HFO5817551.1"
                     /translation="MNQQDIEQVVKAVLLKMQSSDTPPAAVHEMGVFASLDDAVAAAK
                     VAQQGLKSVAMRQLAIAAIREAGEKHARDLAELAVSETGMGRVEDKFAKNVAQARGTP
                     GVECLSPQVLTGDNGLTLIENAPWGVVASVTPSTNPAATVINNAISLIAAGNSVIFAP
                     HPAAKKVSQRAITLLNQAIVAAGGPENLLVTVANPDIETAQRLFKFPGIGLLVVTGGE
                     AVVEAARKHTNKRLIAAGAGNPPVVVDETADLARAAQSIVKGASFDNNIICADEKVLI
                     VVDSVADELMRLMEGQHAVKLTAEQAQQLQSVLLKNIDERGKGTVSRDWVGRDAAKIA
                     AAIGLKVPEQTRLLFVETTAEHPFAVTELMMPVLPVVRVANVADAIALAVKLEGGCHH
                     TAAMHSRNIENMNQMANAIDTSIFVKNGPCIAGLGLGGEGWTTMTITTPTGEGVTSAR
                     TFVRLRRCVLVDAFRIV"
     gene            complement(36842..37129)
                     /gene="eutN"
                     /locus_tag="ACHKBL_000630"
     CDS             complement(36842..37129)
                     /gene="eutN"
                     /locus_tag="ACHKBL_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutN"
                     /protein_id="HFO5817552.1"
                     /translation="MKLAVVTGQIVCTVRHHGLAHDKLLMVEMIDPQGNPDGQCAVAI
                     DNIGAGTGEWVLLVSGSSARQAHKSETSPVDLCVIGIVDEVVSGGQVIFHK"
     gene            complement(37236..37529)
                     /gene="eutM"
                     /locus_tag="ACHKBL_000631"
     CDS             complement(37236..37529)
                     /gene="eutM"
                     /locus_tag="ACHKBL_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutM"
                     /protein_id="HFO5817553.1"
                     /translation="MEALGMIETRGLVALIEASDAMVKAARVKLVGVKQIGGGLCTAM
                     VRGDVAACKAATDAGAAAAQRIGELVSVHVIPRPHGDLEEVFPIGLKGDSSNL"
     gene            complement(37568..38584)
                     /gene="pta"
                     /locus_tag="ACHKBL_000632"
     CDS             complement(37568..38584)
                     /gene="pta"
                     /locus_tag="ACHKBL_000632"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFO5817554.1"
                     /translation="MIIERCRKLALRAPARVVFPDALDQRVLKAAQYLHQQGLATPIL
                     VANPFELRQFALSHGVAMDGLQVIDPHGNLAMREEFALRWLARAGEKTPPDALDKLTD
                     PLMFAAAMVSAGTADVCIAGNLSSTANVLRAGLRIIGLQPGCKTLSSIFLMLPQYSGP
                     ALGFADCSVVPQPTAAQLADIALASAETWRAITGEEPRVAMLSFSSNGSARHPCVANV
                     QQATEIVRERAPKLVVDGELQFDAAFVPEVAAQKAPASPLQGKANVMVFPSLEAGNIG
                     YKIAQRLGGYRAVGPLIQGLAAPMHDLSRGCSVQEIIELALVAAVQRQTEVNRESSLQ
                     TLVE"
     gene            complement(38581..39384)
                     /gene="eutT"
                     /locus_tag="ACHKBL_000633"
     CDS             complement(38581..39384)
                     /gene="eutT"
                     /locus_tag="ACHKBL_000633"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006580 - ethanolamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization cob(I)yrinic acid
                     a,c-diamide adenosyltransferase EutT"
                     /protein_id="HFO5817555.1"
                     /translation="MKDFITEAWLRANHTLSEGAEIHLPADSRLTPSARELLESRHLR
                     IKFIDEQGRLFVDDEQQQPQPVHGLTSSDEHPQACCELCRQPVAKKPDTLTHLSAEKM
                     VAKSDPRLAFRAVLDSTIALAVWLQIELAEPWQPWLADIRSRLGNIMRADALGEPLGE
                     QAIVGLSDEDLHRLSHQPLRYLDHDHLVPEASHGRDAALLNLLRTKVRETETVAAQVF
                     ITRSFEVLRPDILQALNRLSSTVYVMMILSVTKQSLTVKQIQQRLGETQ"
     gene            complement(39381..40082)
                     /gene="eutQ"
                     /locus_tag="ACHKBL_000634"
     CDS             complement(39381..40082)
                     /gene="eutQ"
                     /locus_tag="ACHKBL_000634"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutQ"
                     /protein_id="HFO5817556.1"
                     /translation="MKKLITANDIREAHARGELAMSVVLRASIITPEAREVADLLGFT
                     ITECDESIPVTASVPASASADKTESQRIRETIIAQLPEGQFTESLVAQLMEKVMKEKQ
                     SLEQGALQPSFKSVTGKGGIKVIDGSSVKFGRFDGAEPHCVGLTDLVTGDDGSSMAAG
                     FMQWENAFFPWTLNYDEIDMVLEGELHVRHEGETMIAKAGDVMFIPKGSSIEFGTTSS
                     VKFLYVAWPANWQSL"
     gene            complement(40057..40536)
                     /gene="eutP"
                     /locus_tag="ACHKBL_000635"
     CDS             complement(40057..40536)
                     /gene="eutP"
                     /locus_tag="ACHKBL_000635"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutP"
                     /protein_id="HFO5817557.1"
                     /translation="MKRIAFVGTVGAGKTTLFNALQGNYTLARKTQAVEFNDNGDIDT
                     PGEYFSHPRWYHALITTLQDVDMLIYVHGANDPESRLPAGLLDIGVSKRQIAVISKTD
                     MPDADVAATRKLLLETGFEEPIFELNSHDPQSVQQLVDYLASLTKQEEAGEKTHHSE"
     gene            complement(40549..40884)
                     /gene="eutS"
                     /locus_tag="ACHKBL_000636"
     CDS             complement(40549..40884)
                     /gene="eutS"
                     /locus_tag="ACHKBL_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutS"
                     /protein_id="HFO5817558.1"
                     /translation="MDKERIIQEFVPGKQVTLAHLIAHPGEELAKKIGVPDAGAIGIM
                     TLTPGETAMIAGDLALKAADVHIGFLDRFSGALVIYGSVGAVEEALSQTVSGLGRLLN
                     YTLCEMTKS"
BASE COUNT         9422 a        11536 c        10537 g         9515 t
ORIGIN      
        1 tcatcttttt tcttcatgaa tggatgcaat aaatcaattt tatttctcaa actggaagaa
       61 gcacaataga accatcgatc atctggagtc tttatgaaac tttttcgtat cctcgatcct
      121 ttcaccttaa ccctgatcac ggtggtgttg ctggcctctt tctttccggc cagaggcgat
      181 ttcgtcccct tctttgaaaa tctgaccact gcagctattg ccctgctgtt ctttatgcac
      241 ggcgcgaagt tgtcgcgtga ggcgattatt gctggcggtg ggcactggcg actgcatttg
      301 tgggtaatgt gcagcacctt cgtattgttt ccgattctgg gcgtgctgtt tgcctggtgg
      361 aaaccggtaa atgtcgaccc gatgctctac tccggttttc tctacttgtg cattctcccc
      421 gctaccgtgc agtctgcaat cgccttcacg tcaatggcgg gcggtaacgt tgcggcggcg
      481 gtttgttctg cgtcagcatc cagcctgctt gggattttcc tttcaccatt gctggttggt
      541 ctggtgatga atgttcacgg tgcgggaggc agccttgagc aggtcggtaa aattatgctg
      601 caattgctac tgccgtttgt gttggggcat ctttcccggc cgtggattgg tgactgggtg
      661 tcgcgcaata aaaaatggat tgcgaaaact gaccagacgt ccattctgtt ggtggtttat
      721 acggcattca gcgaagccgt cgttaacggt atctggcaca aagttggctg gggatcattg
      781 ctgtttatcg tggtggtcag ttgcgttctt ctggctatcg tgattgtagt taacgtcttt
      841 atggcacgcc gactgggctt caataaggca gatgaaatta ctatcgtctt ttgtggttcg
      901 aaaaagagtc tggcaaatgg catcccgatg gcaaacattc tgttccccac atcggtgatc
      961 ggtatgatgg tgctgcccct gatgattttc catcagatcc aattgatggt ctgtgcggtg
     1021 ctggcgcgtc gatacaaacg ccagaccgaa cagttacagg cgcagcagga aagcagcgcc
     1081 gataaagctt aaagcggacg cttcaggggc tggaccagtt gcgtcagccc ctcggtttta
     1141 atcagcagcg tgatttgcat tagctcaccg agcttccctg ccgggaactc atctttgcgg
     1201 gcaaaccaca gcagatactc ctccggcagg tcgattaagc gacgcccttt gtacttgcca
     1261 aacggcatta tcgtattggc tatttcaatc agctgctctt tttccatctc agctacccag
     1321 caaacgcagc atttccgttt cgtcgatgac ttcaataccc agttcctgcg ccttcgccag
     1381 tttagatcct gcagcttcac ccgctatcac cagatcggtt ttcttcgaca cgctgcccgc
     1441 gactttcgcg cccagttcga ccagtcgagc tttagcgtca tcacgcgaca tctggcttaa
     1501 gctgcccgta agcaccacgg ttttaccagc aaacgggctg tcaatctctt ccgcgttaat
     1561 caccaccggc gcaggccagt gaacgccttc cgccaacagc tcgctgatga cattgcggtt
     1621 gctttcttcg gcaaagaagt tgtgaacgtg ggaggcaacg acaatgccaa catcaggcac
     1681 cttttgcagc tcttcaatcg aagcggcttc cagcgcttcc agcgtgccga aatatgccgc
     1741 cagacctgct gcggtggcct cgccgacttc acggatgcca agtgcataga ggaagcgagc
     1801 aaaggtggtt tctttcgctt tttccagcgc gttaaccacg ttttgtgccg atttcggccc
     1861 catacgctcc agtccggtca gtttgactgc ggtgagtttg aacagatccg ccggagtgtg
     1921 gacatattct ttttcaacca gttgatcgat gattttgtcg cccattccgt caacatccat
     1981 cgcacggcgg gaaacaaagt gtttcagaga ctctttacgc tgcgcaccgc aaatcaggcc
     2041 accggtacag cgggcaaccg cttcaccttc cacacgctcg acgtcagaac cacataccgg
     2101 acaatgcgtc gggaatacaa cctcacgggt atcttccggg cgttcagaaa gcacgacgtt
     2161 aaccacctgc gggatcacgt cgccagcgcg gcgaataacc actttatcgc caatgcgtaa
     2221 accaagacgt tcgatttcgt cagcattatg cagtgtagcg ttactcacca acacgccagc
     2281 aacatggaca ggttccagac gcgcaacagg cgtaatcgcg ccagtacgcc caacctgaaa
     2341 ctcgacgtca cgcacaaagg tcatctgttc ctgcgccggg aatttaaacg ctaccgccca
     2401 gcgcggggca cgcgcgacaa agccaagctg ctcctgctgt gccagtgagt tgaccttaat
     2461 caccacgccg tcgatatcaa agcccagcgt cggacggtct ttttctactt cgtgataaaa
     2521 cgccagcact tcttccgccg attcacaaag tgttacccga tcactgacag gtaatcccca
     2581 cgctttaaat tgcattaagc gtcccaaatg ggtatccggc aattcaccgc cttccagaac
     2641 accaacgcca tagcagaaga aagtgagcgg ccgcttcgct gtaatacgcg gatcaagctg
     2701 acgcagtgaa ccagctgccg cattacgtgg gttagcaaac actttcccgc ccgtgcgtcg
     2761 cgcatcttcg ttaatctttt cgaaccccgc ctgcggcagg aacacttcac cacgcacttc
     2821 cagacgcgcc gggatattct ctccgtgtag cttcagcgga atggcgcgaa tagtacgcac
     2881 attagacgtg atatcttccc cggtggtgcc atcgccacgg gtggcggcac tgactaaaac
     2941 accattttca tacagaatac tgacggcaag accatccagc ttcagctcac agcaccaggt
     3001 gactttctcg ttgcttttca gacggtcctg cacacgtttg ttgaaagcaa gaaagctttc
     3061 ttcatcaaaa acgttatcca gcgacagcat tggcacttca tggcggatct ggctgaaagc
     3121 cgccagcggc gcagcaccta cacgttgggt aggcgaatca ggcgtaatca gttctggatg
     3181 tttggtttcc agctcgcgca attcgcgcat cagcctgtcg tattcagcgt cgggaatttc
     3241 cggcgcatcc atcacatgat aaagatattc atgatggcga agcgtcgttc gcagttctgt
     3301 cagttgttgt tcgattgatt ccatatcgca ccatcaatgc taaaaacccc cgacaagcgg
     3361 gggttcgaag aggagttaat ttgccttaag tgtatcaggc gttggcgtct ttgacttcgc
     3421 ggatgatgtc ctggtactcg cgcaatttct gcggagtcat catacggcgc tgatcgtcaa
     3481 gaacaacacc gcccacttca tcggcaatat gctgcgcaga ttgcagcatc agcttgaagt
     3541 tctgcagctc gtcaccgtaa gacggtacct gcataaagat agtgacaccc ggagtagtga
     3601 aatccttcat ttcaggatca aaggttcccg gtttcaccat attcgccagg ctgaataacg
     3661 ccgggccgct gccatccggg ctaagatgac gatggtaaat attcatatcg ccaaaaatga
     3721 agcccgcttg ttgaatgctg ttaagaagca gttcaccgtt tagctcgcta ccgtgatgcg
     3781 ccgcgacgtt catgataatc accgcttctt tgcgcttcgg tttatccata actggagccg
     3841 gttccgctac aggctcaggc tgtggtgccg ctacgggttc tgcaggctgg aaagcctgtt
     3901 gtgccggttg cggtgctgaa tgcactggct gcggcgctgg cgcgacctgt ggcgaaactg
     3961 gctgctgcaa cggctgttca ggctgcggct gataggcagg ctgctgcacg ggttgctgca
     4021 ccagctgcgc tggacgcgga gcatgttgcg gcggtacctg cgcttcaggc ggctgctgga
     4081 ccggttgacg cggctgcgca gacgcataag gcggttggta ctggtgttgc ggcgacggac
     4141 gagcagcctc atgctcctgg gcgttagccg gggcatgatt cacgcggtga actcgaacct
     4201 caccaacgcc ctcatcatct tcgacatcct cgtcataaga atcgtcgtca cgttttgact
     4261 tcattcgttt taatggccga tcgcggaaca tagaagatcg ttctttacgg ctggtccaga
     4321 aaccatgtac cagtaaagcg attatggcga tcgcgccaac aatgattaat atcagacgca
     4381 aatcctgcat cattatattc tctgttgttc taacaccttg ccaccacggc aaacatttac
     4441 tcactaagag tatttgtcga ttacctcaag tgcaagtgca ctattaactt tcacagcaca
     4501 aagatagatg aaatcgtgct ttttgctgtt ttttcgaaca tatcctaact gtccattgcg
     4561 caattacccg gtaaaatacg cagaattttc ctgggattgg tcaaaaggag ctcatcctga
     4621 ttatggtttc atcattcaca tctgccccac gcagcggttt ttactatttt gcgcaaggct
     4681 ggaagctcgt ctcgcaacct gggattcggc gtttcgttat tttaccgctg ctggtcaata
     4741 ttttgttgat ggggggcgca ttctggtggc tctttacgca actcgatgtc tggatcccga
     4801 ctctcatgag ttatgttccg gactggttac aatggctgag ctatctgttg tggcccctgg
     4861 ctgtcatctc tgtgctgtta gtatttggtt atttcttttc aacgattgct aactggattg
     4921 ccgctccgtt caacggttta ttggctgaac aactggaagc aagactgact ggcgctacac
     4981 cgccagatac cggaattttc ggtatcatga aagatgtgcc gcgaatcatg aaacgcgaat
     5041 ggcaaaaatt tgcctggtat ctgccgcgcg caattgtatt gctaattctt tacttcatcc
     5101 ctggtattgg gcaaaccgtc gcgccagtac tgtggttcct gtttagcgcc tggatgttag
     5161 ccatccagta ttgcgattac cccttcgata accacaaagt gccgtttaaa gagatgcgca
     5221 ccgccctgcg cacacgcaaa atcaccaata tgcagtttgg tgctttaacc agcctgttta
     5281 cgatgatccc gttgcttaat ctgttcatca tgcccgttgc cgtttgtggc gcgacggcga
     5341 tgtgggtcga ttgctatcgc gataaacacg cgatgtggcg gtaacaatct accggttatt
     5401 ttgtaaaccg tttgtgtgaa acaggggtgg cttatgccgc cccttattcc atcttgcatg
     5461 tcattatttc ccttctgcat atagatatgc taaatcctta cttccgcata ttctctgagc
     5521 gggtatgcta cctgttgtat cccaatttca tacagttaag gacaggccat gagtaagatt
     5581 tttgaagata actcgctgac tatcggtcac acgccgctgg ttcgcctgaa tcgcatcggt
     5641 aacggacgca ttctggcgaa ggtggaatct cgtaacccca gcttcagcgt taagtgccgt
     5701 atcggtgcca acatgatttg ggatgccgaa aagcgcggcg tgctgaaacc aggcgttgaa
     5761 ctggttgaac caaccagcgg taataccggg attgcactgg cctatgtcgc tgccgctcgc
     5821 ggttacaaac tcaccctgac tatgccagaa accatgagta ttgaacgccg caagctgctg
     5881 aaagcgttag gtgcaaacct ggtgctgacc gaaggtgcta aaggcatgaa aggcgcaatc
     5941 caaaaagcag aagaaattgt cgccagcaat ccagagaaat acctgctgct gcaacaattc
     6001 agcaatccgg caaaccctga aattcacgaa aaaaccaccg gtccggagat ctgggaagat
     6061 accgacggtc aggttgatgt atttattgct ggcgttggga ctggcggtac gctgactggc
     6121 gtcagccgct acattaaagg caccaaaggc aagaccgatc ttatctctgt cgccgttgag
     6181 ccaaccgatt ctccagttat cgcccaggcg ctggcaggtg aagagattaa acctggtccg
     6241 cataaaattc agggtattgg cgctggtttt atcccggcta accttgatct caagctggtc
     6301 gacaaagtca ttggcatcac caatgaagaa gccatttcta ccgcacgtcg tctgatggaa
     6361 gaagaaggca ttcttgctgg tatctcttct ggagcagctg ttgctgcggc gttgaaacta
     6421 caagaagatg aaagctttac caataagaat attgtggtta ttctgccgtc atcgggtgag
     6481 cgttatttaa gcactgcatt gtttgccgat cttttcactg agaaagaact gcaacagtaa
     6541 tgccagcttg taaaaaatgc gtaaaaaagc acctttttag gtgctttttt gtggcctgct
     6601 tcaaactttc gcccctcctg gcattgattc agcctgtcgg aactggtatt taaccagact
     6661 aattattttg atgcgcgaaa ttaatcgtta caggaaaagc caaagctgaa tcgattttat
     6721 gatttggttc aattcttcct ttagcggcat aatgtttaat gacgtacgaa acgtcagcgg
     6781 tcaacacccg ccagcaatgg actgtattac gctcttcgtg cgtcgcgtct gttaaaaact
     6841 ggcgctaaca atacaggcta aagtcgaacc gccaggctag actttagttc cacaacacta
     6901 aacctataag ttggggaaat acaatgttcc agcaagaagt taccattacc gctccgaacg
     6961 gtctgcacac ccgccctgct gcccagtttg taaaagaagc taagggcttc acttctgaaa
     7021 ttactgtgac ttccaacggc aaaagcgcca gcgcgaaaag cctgtttaaa ctgcagactc
     7081 tgggcctgac tcaaggtacc gttgtgacta tctccgcaga aggcgaagac gagcagaaag
     7141 cggttgaaca tctggttaaa ctgatggcgg aactcgagta atttcccggg ttcttttaaa
     7201 aatcagtcac aagtaaggta gggttatgat ttcaggcatt ttagcatccc cgggtatcgc
     7261 tttcggtaaa gctctgcttc tgaaagaaga cgaaattgtc attgaccgga aaaaaatttc
     7321 tgccgaccag gttgatcagg aagttgaacg ttttctgagc ggtcgtgcca aggcatcagc
     7381 ccagctggaa acgatcaaaa cgaaagctgg tgaaacgttc ggtgaagaaa aagaagccat
     7441 ctttgaaggg catattatgc tgctcgaaga tgaggagctg gagcaggaaa tcatagccct
     7501 gattaaagat aagcacatga cagctgacgc agctgctcat gaagttatcg aaggtcaggc
     7561 ttctgccctg gaagagctgg atgatgaata cctgaaagaa cgtgcggctg acgtacgtga
     7621 tatcggtaag cgcctgctgc gcaacatcct gggcctgaag attatcgacc tgagcgccat
     7681 tcaggatgaa gtcattctgg ttgccactga tctgacgccg tccgaaaccg cacagctgaa
     7741 cctgaagaag gtgctgggtt tcatcaccga cgcgggtggc cgtacttccc acacctctat
     7801 catggcgcgt tctctggaac tgcctgctat cgtgggtacc ggtagcgtca cctctcaggt
     7861 gaaaaatgac gactatctga ttctggatgc cgtaaataat caggtttacg tcaatccaac
     7921 caacgaagtt attgataaaa tgcgcgctgt tcaggagcaa gtggcttctg aaaaagcaga
     7981 gcttgctaaa ctgaaagatc tgccagctat tacgctggac ggtcaccagg tagaagtgtg
     8041 cgctaacatt ggtacggttc gtgacgttga aggtgcagag cgtaacggcg ctgaaggcgt
     8101 tggtctgtat cgtactgagt tcctgttcat ggaccgcgac gcgctgccca ctgaagaaga
     8161 acagtttgct gcttacaaag cagtggctga agcgtgtggc tcgcaggcgg ttatcgttcg
     8221 taccatggac atcggcggcg acaaagagct gccatacatg aacttcccga aagaagagaa
     8281 cccgttcctc ggctggcgcg ctatccgtat cgcgatggac cgtaaagaga tcctgcgcga
     8341 tcagctccgc gctatcctgc gtgcctcggc tttcggtaaa ttgcgcatta tgttcccgat
     8401 gatcatctct gttgaagaag tacgtgcact gcgcaaagag atcgaaatct acaaacagga
     8461 actgcgcgac gaaggtaaag cgtttgacga gtcaattgaa atcggcgtaa tggtggaaac
     8521 accggctgcc gcaacaattg cacgtcattt agccaaagaa gttgatttct ttagtatcgg
     8581 caccaatgat ttaacgcagt acactctggc agttgaccgt ggtaatgata tgatttcaca
     8641 cctttaccag ccaatgtcac cgtccgtgct gaacttgatc aagcaagtta ttgatgcttc
     8701 tcatgctgaa ggcaaatgga ctggcatgtg tggtgagctt gcaggcgatg aacgtgctac
     8761 acttctgttg ctggggatgg gtctggacga attctctatg agcgccattt ctatcccgcg
     8821 cattaagaag attatccgta acacgaactt cgaagatgcg aaggtgttag cagagcaggc
     8881 tcttgctcaa ccgacaacgg acgagttaat gacgctggtt aacaagttca ttgaagaaaa
     8941 aacaatctgc taatccacga gatgcggccc aatttactgc ttaggagaag atcatgggtt
     9001 tgttcgataa actgaaatct ctggtttccg acgacaagaa ggataccgga actattgaga
     9061 tcattgctcc gctctctggc gagatcgtca atatcgaaga cgtgccggat gtcgtttttg
     9121 cggaaaaaat cgttggtgat ggcattgcta tcaaaccaac tggaaacaaa atggtcgcgc
     9181 ctgtagacgg caccattggt aaaatctttg aaaccaacca cgcgttctct atcgaatctg
     9241 atagcggcgt tgaactgttc gtccacttcg gtatcgacac cgttgaactg aaaggcgaag
     9301 gcttcaagcg tattgctgaa gaaggtcagc gcgtgaaagt tggcgatact gtcattgaat
     9361 ttgatctgcc gctgctggaa gagaaagcca agtctaccct gactccggtt gttatctcca
     9421 acatggacga aatcaaagaa ctgatcaaac tgtccggtag cgtgactgtg ggtgaaaccc
     9481 cggttatccg catcaagaag taattcttgc cgcagtgaaa aatggcgccc atcggcgcca
     9541 ttttttatgc ttccgccagc ggcggcaaaa tcaattcatc gctctcatgc tgctgggtgt
     9601 agcgcatcac ttccagtacg cgcaaccccg cacggtgcac cgcatcgttt aacgccttcc
     9661 ctttcagcaa tccactgata agctgagcac aaaacaggtc gccagtccct ttcaggtcgg
     9721 tttttacccg tgaatgggaa atgacattca cgctgtcggc actgaccacc accacctgca
     9781 tctcctgatt ttcttcatta ccggaggcgc tggtaatcac cacccatttt aatgtgtctg
     9841 aaagcagact ttttgcggca gcaatggcac tgtcgagatc gcggcaattt ttaccggtca
     9901 ggatttccag ctcaaagata ttgggggtaa ttccctgcgc cagcggcagt aaatattgtc
     9961 gatacgcctc gggaaggtca ggtttgacat aaattccgct atcaatatcg ccaatcaccg
    10021 gatcgaccat gatcaatagg tcaggatggt ctttgcgtag cgcagtcagc cactcggcaa
    10081 ggattttgat ttgcgatgcc gttcccatat agcccgtggt tacagctcga agttggcgca
    10141 gcgcatcacg ctcctgaagc gcacgcaaat agccgctaaa ccattcatcc ggaatcgcac
    10201 caccgtagaa agtgtcataa tgcggtgtat tgctcagcaa taccgtcggc acggcaaaga
    10261 cattcaggcc gttctgtttg atagcaggca ccgcaatgct gttgcctacg ctgccgtaca
    10321 ctacctgcga ctgcacggcg acgatatccg cctgcaatgc cctgctctta tcgttaaaca
    10381 acaacaaact actcatttaa ttttttctcc ttgccgatga tcctcatcgt attccaaccg
    10441 aaactttacc tgattctggc agtcaaatcg gctatcacaa aacaaggata aggtaattca
    10501 atgaagaaaa tcatttgtct ggtcattacg ctgctaatga cactccccgt ttacgcgaag
    10561 ttaactgccc atgaagaagc ccgtatcaac gccatgctgg agggattagc acagaaaaag
    10621 gatttgatat ttgtgcgtaa cggcgatgat catacctgcg atgaagcggt ttcccacctg
    10681 cgtctgaagc tcggcaatac ccgtaaccgc attgacactg ccgagcagtt tattgataag
    10741 gtagcctcgt cgtcatcgat taccgggaag ccgtatatcg tgaagatgcc cggtaaaagc
    10801 gatgagaacg cacaaccttt tttacatgcg ctgattgcgc agacggataa aacggtgcct
    10861 gcgcaataat cttaaatccc cgccccctgg ctaaaatgct cttccccaaa caccccggta
    10921 gaaaggtagc gatcgccgcg atcgcagatg atcgccacaa ccaccgcgcc ggggttagct
    10981 tttgccacgc gcagtgctcc ggcaaccgcg ccgccggagc tgacgccaca gaatattcct
    11041 tcccgcactg ccagttcgcg catggtgttt tccgcatcgc gctgatgaat atccagcacc
    11101 tcatccacca gcgaagcgtt gaaaatcccc ggcagatatt ccgcaggcca gcggcgaatg
    11161 ccgggaatgc tactgccctc ttccggctgc aggccaacaa tggtcaccgg tttggattgt
    11221 tcgcgcataa agcgtgaaac gccggtgata gtgccggttg ttcccatgct ggagacaaaa
    11281 tgggtaatgc gcccgccggt ttgctgccag atttccggcc cggtggtggt gtaatgggcg
    11341 aagggattat caggattatt gaactgatcg agcagctttc cttcaccacg attggccatc
    11401 tccagcgcca gatcgcgcgc gccttccatg ccctgctctt tggtgacaag aatcagttcc
    11461 gcaccgtatg ccagcatcgc tgcacggcgc tcctggctca tgttgtcggg catcagcaat
    11521 ttcatgcgat agcctttaag cgcggcaatc attgccagcg caatgccggt gttaccactg
    11581 gtggcttcaa tcaggacatc gcccggtttg atttctccgc gcttttccgc ctcgacgatc
    11641 atcgaaagcg ctgcacgatc tttcaccgaa cctgccgggt tattgccttc cagtttcaac
    11701 cagacttcac tgccgttatc cggcgccatt cgctgcaact tcaccagagg cgtattgcca
    11761 attgtttgtt ctaatgtact cacgatctct gtccatacgt ggtgttgcct gatgcgacgc
    11821 ttgcgcgtct tatcaggcct acaggttaca aaccttgcca ttaaaaagcc cggatcgcgg
    11881 gaagcgcctc cgggcgttta acattcactc aacctatcag gcgctttgtg cgagagcaag
    11941 ttcctcatcg cgggtttcaa tacgctcgtc gccgttatac agccgcgcat gttgcagacc
    12001 aacgaataaa cgctcgccac gctgcggggc atcgtcgcca tgcatcacaa ccgtcagcgg
    12061 ttcgttgtac caccccagcg gctgcacgac taattgggtg tagtggcctt tcgggctggc
    12121 ttccagtacc tgtaccggca gcggcgaatc gaggctggta cggcggctga tatccacttc
    12181 ccacggacgc aggaagagat ccaccggccc ctgatacgca ggtgtatagc ccagcggcca
    12241 gcgatgtgcg ccaacatgga actgcccgcc gcgaatggtt ccctgcaggc ggttcacttc
    12301 gcccataaat tcgagtacaa aacgggtcgc tggttcgcgc catacctggt ccggtgcatc
    12361 agcctgctcg atattgccct ggctcatcac cactacacga tcggctactt cggtcgcttc
    12421 ttcctggtca tgggtcacaa acacgctggt gaatttcagc tcttcatgta actgacgcag
    12481 ccagcgacgc aactctttac gcacctgcgc atccagcgcg ccaaacggtt catcaagcag
    12541 cagaatttgc ggttctacag ccagcgcgcg cgccagtgcc acacgctgtt tctgaccgcc
    12601 ggaaagctgt gctggataac gatccgccag atgcgcaagc tgcaccatct ccagcaattt
    12661 tgtcactttc gctttgatgg ctgcggcatt cgggcgctcg cgacgcggca gcaccgtcag
    12721 gccgaaagcg atattgtcga acaccgtcat atggcggaac agcgcatagt gctggaacac
    12781 gaaaccgact ttacgatcgc gcgcatgcag gcggctgacg tcggtgccgt ggaagcgaat
    12841 atgcccgctg gtttgatgct ccagcccggc gataatgcgc agcagcgtgg ttttcccgga
    12901 accggacggc cccagcaacg cgaccatctg accagaagga atatccagtg agatatcgtt
    12961 cagcacctgg gtgcgaccaa aagacttctt aatattggca atctcaatgc tcatgatgtt
    13021 cctcctgctg cgcgcgtttt tcctgattct ccagacgcca ctgcaacata ctctttaaaa
    13081 acagggtgat aatcgccatc agcgtcaaca acgcggcagc ggtaaaggag ccgacggtgt
    13141 tgtagtcttg ctccagcaat tcaatctgta acggcagcga aagtgtttcg ccgcgaatcg
    13201 agccggaaac caccgacacc gcaccaaact cgccaatcgc gcgggcgttg gtcagcacca
    13261 cgccataaag cagcgcccag cggatgttcg gcagcgtgac gcggcgaaac atctgccagc
    13321 cagacgcgcc aagcaaaatc gccgcttcgt cttcctggct gccctggctt aacatcaccg
    13381 gcaccagctc acgcaccaca aacggacacg tcacgaagat ggtgaccagc accattcccg
    13441 gccaggagaa cataatttgc agattatgtt catccagcca gccgcccagc gggccgttag
    13501 agccgtagaa cagcagatac accagaccag caaccaccgg cgacacggca aacggaatgt
    13561 ccaacagcgt cagcaatagc tggcgtccgg ggaagttaaa gcgcgtcacc agccaggcca
    13621 gcagaatacc gaacaccagg tttaccggta cggcaatcag cgcgatcatc accgtcagcc
    13681 agatggcgtg cagcatgtcc ggatcggcca gattctgtaa aaccggcatc agccctttgc
    13741 tgaacgcctg tacgaagata taaatcatcg gcaccagcag gatgaacgcc gataccagca
    13801 tcccgatgcc aatcagaaac catttgcccc agttaatcgg gcgcgcgtca taacgcttca
    13861 actgtgtcac ttccgccatt aatgacctac cacacgccga ccaaagcgac tttgcagagt
    13921 gttaattgag aacagcagca gcagcgatgc cgcgaggatc accgaggcaa tcgcgctcgc
    13981 cgccgggtaa tcaaactcct gtaagcgaac aaagatcatc agcgacgtca cttctgtttt
    14041 ccaggcgata ttcccggcga taaaaatcac cgcgccaaac tcaccgaggc tgcgggtaaa
    14101 cgacagcgcc acacccgcca ccagcgccgg agatagttcc ggcagcacca ctttgcgaaa
    14161 actctgccag cgtgttgcgc cgagcgtttc tgccgcctct tcatattccg ggcctaactc
    14221 ttccagcacc ggctgcacgg tgcgcaccac aaacggaatg ctggtaaagg ccatcgccac
    14281 cgcaatcccc agccaggtat aggtgacttt gatatcaaac ttcgccagcc attcaccgta
    14341 aaaaccgttc acggaaaaga gcgaggccag cgttaaaccg gcgactgccg ttggcagcgc
    14401 aaagggtaaa tccatcagcg catcaagcag cgtgcgaccc ggaaagcgat agcgggttaa
    14461 gatccacgcc atcagcagac cgaaaacgcc gttaaaaatc gatgccacaa acgccgacag
    14521 cagcgtcact ttataggctg cgaccacctg cgggttggtg atcacctccc agtactgcgc
    14581 ccagctcatc tgggccagtt gcatcaccag cgcggagagc ggcagcagca aaatcaggca
    14641 cacaaacagc agactggtgc cgaggcttaa ggtaaagccc ggcagcacgc gtcttgaaga
    14701 aactgcaaac attacttacg ccccgccgct aacagcttgt ctaactcgcc gccgctggtg
    14761 aagtgggttt tcatcacttc cggccaggag ccaaatttat cttccacgcg gaacagctcg
    14821 gtctgcggga atttatcttt cagtttgccc atgacttccg ggttatttac gcggtagtaa
    14881 tagtcggtga tgatggtttg cgcctgcggg ctgtagagcc agttcaggta ggcttttgcc
    14941 gctttttccg tgccgttggc ctgtacgttt ttatccaccc acgccaccgg gaattccgcc
    15001 agaatgttgg ttttcggaat caccacttca aagccctgcg cttcatactg tttacggatg
    15061 ttgttcactt ccgactcgaa gctgatcagc acatcgccca ggccgcgctc ggcaaaagtg
    15121 gtggtcgcgc cacgaccgcc agtatcgaac acttcaacgt ttttcaggaa ctgggtcata
    15181 aactgttcgg ttttggcttt atcaccaccg tcggctttgt ccgctgcgcc ccatgccgca
    15241 aggtaggtat aacgcgcgtt acccgacgtt ttcgggttcg ggaaaatcag cttcacgtca
    15301 gagcgtacca ggtcgttcca gtcgtggata ttctttgggt tgcccttacg caccaggaag
    15361 cccatggtgg agtagaacgg cgagctatta ttcggcaggc gcgactgcca gtccgctggg
    15421 atcagcttgc ctttatcgtg caggatttgt acgtcggtca cctggttata agtgacaacg
    15481 tcggctttta aaccctgcaa aattgccagc gcctgttttg atgatccggc atgagattgt
    15541 ttaatcgtca gtttgtcgcc accgttatct tttgcccatt gttgctcaaa cggcgggttc
    15601 agggcggcaa acagctcgcg ggagacgtca taagaactgt tcagcagttc cgttgcctgt
    15661 acatggcccg ccagtagcag agaagcaacc agcacgagtg agttcttttt cagtaagtta
    15721 acggccattg cgcaccctta taaatttaat gactttctaa tagccatcat atttataacg
    15781 gacgtgaaag gagtaacggt tttatatacc gtttggtgat ttggaagttg aaaagggaat
    15841 aagacgaaat cctccccaca aatgcaggga ggaaacagag gtgaatcaga tgccgacgct
    15901 aaccgtctcc ggcagtgtgc tgccgccatc aatcacattc tgtgtaccgg ttaaatagct
    15961 ggattcatcc gatgcgagga aggccgccag ttcgccgact tccagcggat cggcgaggcg
    16021 acgcatcggg attgctttcg ccatttcagt cagcaccgat tctggatctt ccgggttcga
    16081 ctggcgggca atgctttccg ccattggcgt gcgaacgtat ccagggcaga tggcgttaac
    16141 gcgaatacca gactgcgcgt actccaccgc cagcgattta gtcaggccaa caatcgccgc
    16201 tttcgttaag gcgtacgccg tttcgcccgg gtctgccacc atatcgccag tgactgaaga
    16261 catcatcaca atccggccat ctttgcgggc aatcatctcc ggcagcaccg ccttcgtgac
    16321 gttccatacg ccttttatat tgatatcaat atggaaatcg cgatcttcat cgctcatatc
    16381 aaggaaactg cccagacgac aaacgcctgc gttattcacc agaatatcaa tgcgcccttc
    16441 tttttcctta gcgcgcttaa tagctgcggc gaccgacgcc gggtcacgca catcggcgac
    16501 aaccgccgta cagcgatgac cacggccaca cagttcgtcc gccagctttt cgatctcagg
    16561 ggagatatcc agcaagatta ggtttgcgcc atgacgcgca aaagttctgg caattccttc
    16621 gccaattccc tgcaatgcgc ccgtaatcag tgctgtcttg cccgtgagtt tacccatttt
    16681 aatgctctcc ttgttaatac agagcgttac actccattcc ctctgaaaat acaggataaa
    16741 caaaatattg ctgcggatca gctcactaat tcaggaaaat ctcaatccag tcgctgcttt
    16801 atgcatcatt tcaggcgttg cgttagctca ctgctgcgct caatcatttt caatgactcc
    16861 acatcattga gttgcacaag gccaacaaac agtaaatcgg taacagaatt ttgcgccgtg
    16921 cgggtggaca tcgacgagct gcgccattcg gtttcgccag aaacggtatc gagggtgtaa
    16981 tgcgccagcc gccgcagcgg ggaatcggtt aatgaggtaa tggcaatcac cgttgccccc
    17041 tgctttcgcg ctgcctcggc gcacaagaca atctcttttt tactgccact gtaagagata
    17101 gcgatttgta catcgccttt ttttagcgcc tgagaaacgg tggcttgtac gtgggtgtcg
    17161 gcctcacagg ccacgcgata accaattttc atcagtttga acgacagatc gcgccccacc
    17221 agctccgacc cgcccaggcc cgttatctgg ataaacggtg ctttactgat gacctcaatg
    17281 attttttgca gccgcgcgta gtcaaacaac gcgcaggttt gctccagcgc ctgctctttt
    17341 tcgcgattca gtttacgggc aatcacttcc agcgaatcat cactggtaat cgaactgtgc
    17401 aggtgcagcg ccgtggcatt cgttttctcc cggctggcac tgtattcgcc aattaacgcc
    17461 atacgcaatt cagtaaatcc ttgcgcgccg agcttctggg caaacttcac aatactcgac
    17521 tggctgatgc cgagctgttt cgccatcttg cgcgaagaga cggatttcag ctcactcacg
    17581 ttggcccgca gaaagtcagc aattttttgc tcgttcccgg taaattcact ttccgcattg
    17641 cgtattttcg tcaggtacaa catcttccac tctcctgggc tggcagcaaa aattccgctt
    17701 aagttcttat gacgctcttc acactctgcg agtgtagact caatgattcc tttaagtctg
    17761 tcaattcggg atatatgatt ccatacatct gcaattattt gatcgtaaat agtaaggtca
    17821 ccaccgatgc aacttgaaaa gatgattacc gaaggctcga acgccgcttc ggctgaaatt
    17881 gaccgcgtat caacgctgga aatgtgccgg attatcaacg atgaagataa aaccgtacca
    17941 ctcgccgttg agcgcgtact gccggatatc gccgcagcga tcgatgttat ccacgcccag
    18001 gtcagcggcg gcggtcgtct gatttatctc ggtgcgggaa catcaggtcg tctggggatt
    18061 ctggatgcca gcgaatgtcc gccgacctac ggcgtgaaac cgggtctggt ggttggatta
    18121 attgctggcg gcgaatatgc cattcagcac gcggtggaag gcgcggaaga tagccaggaa
    18181 ggcggtgtga atgatctgaa aaacatcgga ttaaccgcgc aggatgtggt ggttggtatt
    18241 gccgccagtg gtcgcacgcc gtatgtgatt gccggactgg aatacgcgcg ccagctcggc
    18301 tgccgcacag tgggaatttc ctgtaatcca gggagcgcgg tttccaccac cgctgagttt
    18361 gctattacgc cgatcgttgg tcccgaggtg gttaccggtt cttcacgaat gaaagcaggc
    18421 acagcgcaga aactggtgct caatatgctc tccaccgggc tgatgattaa atccggcaaa
    18481 gtgttcggca acctaatggt cgatgtggtc gccaccaacg aaaaattgca tgtgcgccag
    18541 gtcaatatcg tcaaaaacgc taccggatgt agcgcagagc aagcggaagc agcgttgatt
    18601 gcctgcgagc gcaactgtaa aacggccatt gtgatggtgc tgaaaaatct cgatgccgca
    18661 gaagctaaaa aacgcctgga tcaacacggc ggctttattc gtcaggtttt agacaaggaa
    18721 taacccatgg ccaaagagat cagcagtgaa cttctgaaca ccattcttac ccgggtcggt
    18781 ggaccgggaa atatcgccag ttgtggtaac tgtatgacgc gcctgcgtct gggtgtacat
    18841 gacagttcac tggttgatcc caatatcaaa acgctggaag gcgtgaaggg cgtcatttta
    18901 accagcgacc aggtgcaggt cgtttttggg cctggtaaag cgcatcgtgc cgcaaaagcg
    18961 atgagcgagc tgctgggaga agcaccggta caggatgccg cagaaatcgc cgcccagaac
    19021 aaacgtcagt taaaagccaa acaaacctcc ggcgtgcaac aatttctcgc caaattcgcc
    19081 accatcttca cgccgctgat ccccggtttt attgccgctg gtctgctgct ggggatagcg
    19141 acgttaattg ccacggtgat gcacgttccg gcagatgctc agggcacgct acccgatgcg
    19201 ctgaatttta tgaaggtgtt cagcaaaggc ctgttcacct tcctggtgat tctggtgggc
    19261 tataacgccg cccaggcatt tggcggcacg ggcgtcaatg gcgcaattat cgccgcgctc
    19321 tttttgctcg gttataaccc aactgcaacc accggttact acgccggatt tcacgatttc
    19381 tttggtctgc ccatcgatcc gcgcggcaat attatcggcg tgttgattgc cgcgtgggcc
    19441 tgcgcccgca tcgaaggcat ggtgcgccgc tttatgccgg acgatctcga catgctgttg
    19501 acctcgttaa tcaccctgct aatcaccgct acgcttgcgt acctgatcat tatgccgctg
    19561 ggcggctggc tgtttgaagg catgtcgtgg ctgtttatgc acctgaacag taatccgctc
    19621 ggttgtgcag tattagccgg gctgttcctg atcgccgtgg tgtttggcgt gcatcagggc
    19681 tttattcccg tttacctcgc gttaatggac agtcagggat tcaacagctt atttcccatc
    19741 ctttcaatgg caggcgcggg ccaggtgggc gcggcgctgg cactctactg gcgggcgcaa
    19801 ccgcacagtg cgctacgcag ccaggtgcgc ggggcgatta ttcccggtct gttgggcgtt
    19861 ggcgaaccgc tgatttacgg cgtcaccctg ccccgcatga aaccgtttgt taccgcctgt
    19921 ttaggtggcg cggcgggtgg tttgtttatc gggctgatag cctggtgggg cctgccgatg
    19981 ggcttaaaca gtgcctttgg gccgtctggt ctggtggcgc tgccgctgat gacatcagca
    20041 caaggtatcc ttccggcaat ggcggtttat gcaggcggga ttctggtggc atgggtttgc
    20101 gggtttattt tcaccacgct ctttggctgc cgtaacgtca atctggactg ataatatgaa
    20161 acggacaatg ctctatcttt ctctgctggc tgtcagctgt agcgtcagcg ccgcaaaata
    20221 ccctgttctg acagaaagtt cgccagagaa agcagggttt aacgtcgaac ggcttaatca
    20281 gatggatcgc tggataagcc agcaagtgga cgccggttat cccggcgtaa acctgctgat
    20341 tataaaagat aatcagattg tttatcgcaa agcctggggc gcggcgaaaa agtacgatgg
    20401 cagcgcgtta atggcgcagc ctgtcaaagc caccaccggg acgctgtatg acctggcttc
    20461 caataccaag atgtacgcca ctaacttcgc cctgcaaaag ctgatgagcg aaggcaaact
    20521 gcatcctgac gatcggattg cgaaatatat tccagggttt gccgacagcc cgaacgatgc
    20581 gatcaaaggc aaaaacacgc tgcggatttc tgatctgctg catcacagcg gcggtttccc
    20641 ggcagatccg caatacccga ataaagcggt cgcgggcgcg ttatattccc aggataaagg
    20701 ccagacgctg gagatgatca agcgcacacc gctggagtat caaccctgca gcaagcatat
    20761 ctacagcgat gtcgattata tgctgcttgg atttatcgtc gagtcggtta ccggtcagcc
    20821 acttgaccgc tatgttgaag agtcgattta tcgcccgctc ggcctgacgc acacggtatt
    20881 taatccgctg ctgaaaggct ttaaaccaca gcaaattgcc gccacggaac tgaacggcaa
    20941 tacccgcgat ggcgtgattc acttcccgaa tatccgcact tccactctct ggggtcaggt
    21001 gcacgatgaa aaagcctttt attcgatggg cggcgtttcc gggcacgcag gtttgttttc
    21061 cgataccggc gatattgcgg tgttaatgca aacgatgctg aacggcggtg gctatggtga
    21121 tgtgcagctg ttcaatgcgg aaacggtgaa gatgttcacc accagctcta aggaagatgc
    21181 cacttttggc cttggctggc gcgtgaatgg taatgccacc atgacgccga cgtttggcac
    21241 gctggcaagc ccgcaaacct acggtcacac tggctggacc gggacggtga ccgttatcga
    21301 tccggtgaat catatggcga ttgtgatgtt aagcaacaag ccacattcgc cagtagccga
    21361 tccgcaaaag aatcccaata tgtttgaaag cggtcagttg ccgattgcaa cttatggttg
    21421 ggttgttgat caggtgtatg cggcgttaaa gcagtaataa aaaggccagt cagttgtgat
    21481 actggctggc ctttaatcat cgaatcagaa acgcttacaa cgccattaac ttgtccagcg
    21541 acggcgcgaa gtaatagccg ccagtgaccg gtttggtaaa acgcaacatc gcatcacgtt
    21601 tgccatcggt atcgccaaac atgctcagca gttgctgctc aatgttatgc agacgcgcgc
    21661 agtaggcgca gaagtacaga ccgtgcgtac cgctggcggt gccgtacggc aggctctgtc
    21721 gaacaatctt cagccctttg ccatcttctt tcagatcaac gcgggtcagg tgagaggttt
    21781 ccggacgttc gtcgccgtca atctcttcgt tggcctcttt agtgcgcccg atcatcatct
    21841 cttgatcgtg aatgctcatc cggttgagtt gcttcaggtt gtgctcccaa cgctgcacaa
    21901 acacatagct gccgccggca tccacgccat ctttgataac cgccacttcg cggcgcgtct
    21961 cttcacccgc cgggttttct gtaccgtcaa caaagccgct caggtcacgc tcttcaaccc
    22021 aacggaagcc gtggatctct tctttcactt caatgcaatc accaaacgct tccatcgccg
    22081 cctgggcgac agagaagttt acgtcgtgac gcagagaaag aatgtggatc aacacatcga
    22141 actgggtcgt cggtgcaaga cctttaccgt agcccggaaa atctttcagc tcttcggccc
    22201 caacgccgcc gctcagagcg cgccaggtat tgttaccaaa ggcaaccacc gcaccaagat
    22261 gcgcgtccgg gaatttcgct tcaaaagttg ccagcttgtc ggcaaatgtt ttactggccg
    22321 cacgcaggac gtcaacttcc cctttcacgt tggcttcgat ccaaatcgcc gcgcggcaat
    22381 gttctggcaa aatgccactc tgaacctgag acattgttcc tcctgaaaat aagaatgcca
    22441 cgcaatccgt ggcattgttg ggcgctattt tacgtgtttt ttcgcggcgg cggtttgttc
    22501 agacgcaaat taacgccgcc agataatttt actgactttc cagtttttca gggtatcgtc
    22561 cgaaggcatt aacccttcag gaccactcca ttccccgctg aactggtagc taatatgttg
    22621 actgccttcg gctttacatt cgacggcacg attatcatca ccatcagctt tttggcaatt
    22681 gccaaatgct ttgctgtaaa ggtcgctaaa cggtgtaccg attttaacac cggtgtcagc
    22741 aggaatatcg ctatccagca cgtcaatgcg actgatcgtg ccttgatcgc cgttaatcac
    22801 catcgccacg ttgtcgccct tcatcacttc aaaaaagcgc accacgttgc cgttcgcggt
    22861 tttcattccg ctgcgcaggc gataatcgcc atcaagcgca tcggcaatgg cttgttcttg
    22921 cagtggtgtg gacgccgtta attcaccaac gccctgctcg ctcactttgg tggatgatcc
    22981 aaaccagttc cacggattag cggcagacca gttaacggac gacatcgtgg aacagccggt
    23041 cagcatcaac ggcatagcgc ataacattaa acgcagcgat ttcatgtcac ttcctttggt
    23101 tattcaataa cgttgcttgg agtgcaaatt caccaaaaag tgccgttatt ctttttcttc
    23161 attaaaacaa gcgcgtaaac gtcgattggt cagcagccag attaacgcca caatatccgc
    23221 cactaccagc gccagaccga taccgctaac ggattcaccg ttcagccaca accacggctg
    23281 ccagcaaagt aaaaccactt gcgccaacag caacagaaaa tagagcacat gccagatacg
    23341 gggaaatgaa gcccgccgac cactcagcaa aaacgccagc accgccggaa tgccgggaat
    23401 caaccccagc cagaaattat cgtgatcggg ataaaacaga tttagcagtg cagtaccctg
    23461 ctcgcgcgac gcaccggcaa tgacaaacag cacccaggtt cgcgcctgaa gcaatagcac
    23521 aagccagaag agcaagggta aacgcaggcg accgtacgca tcataatgga caggatgaaa
    23581 ctcagtactc ttcatcttca atcaaacgct tacccagact cagcacgtcg gcgtgttcat
    23641 atcccaggcg ttcatacatt ccgagcacca tgtcgttatc ttccggcacg ttgatctgaa
    23701 ttttcgggca gccgcgagca atcagctttt tctccaaccg attaagcaac gcattagcaa
    23761 tcccacgccc acgaaactct ggatgcacgc caagataata agcagacccg cgatgcccgt
    23821 cataaccgcc catcaccgtt ccgaccactt caccgttcac ctcagcgacc agaaacaaac
    23881 tgacgtcatg gttcatctta cgctcaatgt ccatttccgg atcgttccac ggacgcagca
    23941 agtcgcaacg ctcccaaagg gtgatgacct cttcgaaatc ttcctggcga aatacgcgta
    24001 tctccatggt attcgttacc tttttgcggg ttaaaaggct gattatggcg tgaacggtcg
    24061 aattagccaa tatctgacga aaatcggttg aaaaagtggc ataatgggga gttgtcaact
    24121 attgaaatga aaagtaaaac aattctcaac agcaaacggt cgtaacggat tacgcgatac
    24181 gatataacat ctggaacttt attattacaa ctcaggccgt atgagcactt ttaaaccact
    24241 aaaaacactc acttcgcgcc gccaggtgct gaaagcagga cttgcagcgc tgacgttgtc
    24301 aggaatgtcg caagccatcg ccaaagaaga acctttaaaa accagcaacg gacacagcaa
    24361 gccgaaagca aaaaaatctg gtggcaaacg tgtcgttgtt ctcgatccag gccacggcgg
    24421 gattgatacc ggagcgatcg gacgcaacgg ttcaaaagaa aaacatgtgg tgctggcaat
    24481 tgccaaaaac gtccgttcca ttttgcgtaa tcatggcatt gatgcgcgtt taacgcgttc
    24541 tggcgatacg tttatcccac tgtacgatcg cgttgaaatc gcccataaac atggcgcaga
    24601 tctgtttatg tcaattcatg ccgacggctt taccaacccg aaagctgccg gtgcttcggt
    24661 atttgccctc tctaaccgtg gggcaagtag cgcaatggcg aaatacctgt ctgaacgcga
    24721 aaaccgcgcc gatgaagttg ccggtaaaaa ggcgactgac aaggatcacc tattgcagca
    24781 agtgctgttt gatctggtgc aaaccgatac tattaaaaac agcctgacgc tcggctcgca
    24841 tattctgaaa aagattaagc cggtgcataa actgcacagc cgcaacaccg aacaagccgc
    24901 gtttgtggtg ttgaaatcgc cgtcggttcc ttcggtgctg gtggaaacct cgtttatcac
    24961 caacccggaa gaagagcggc tgttaggcac ggcggcattt cgccagaaaa tcgccacagc
    25021 gattgccgaa ggcgtgatca gttatttcca ctggtttgac aaccagaaag cacactcgag
    25081 aaagcggtaa gtgatgaaac ccgacgcaca ccaggttaaa cagtttctgc tcaaccttca
    25141 ggatacaatt tgtcagcagt tgagcgccgt cgatggcaca gaatttgtcg aagatagctg
    25201 gcagcgcgaa gctggcggcg gcggacgtag ccgggtgttg cgtaatggtg gtgttttcga
    25261 acaggcgggt gtcaactttt cacatgtcca cggtgaggcg atgcctgctt ccgccaccgc
    25321 tcatcgcccg gaacttgccg ggcgcagttt cgaggcgatg ggcgtttcac tggtagtgca
    25381 tccgcataat ccgtatgttc ccaccagcca cgcgaatgtg cgctttttta ttgccgaaaa
    25441 accgggtgcc gaacccgtct ggtggtttgg cggcggcttc gacttaaccc ctttctatgg
    25501 ttttgaagac gatgctattc actggcaccg caccgcccgt gacctgtgcc tgccatttgg
    25561 cgaagacgtt tatccgcgtt acaaaaagtg gtgcgacgac tacttctacc tcaaacatcg
    25621 taacgaacaa cgcggcattg gcgggctgtt ctttgatgat ctgaacacgc cagatttcga
    25681 ccactgtttt gcctttatgc aggcggtagg caaaggctac accgacgctt atttaccaat
    25741 tgtagagcga cgtaaaacga tggcctacgg cgagcgcgag cgcaatttcc agttatatcg
    25801 tcgcggtcgt tatgtcgagt tcaatctggt ctgggatcgt ggcacactgt ttggcctgca
    25861 aaccggcggg cgcaccgagt ctatcctgat gtcaatgccg ccgctggtac gctgggagta
    25921 tgattatcag ccagaagatg gcagcccgga agcggcgtta agtgagttta ttaaggtcag
    25981 ggattgggtg taactccctc ccccccattc ccgcatccgc tgatgcagcg tcagtgacgg
    26041 cttctcggca aacagctgct ggtaatccgt ggcgaattgc cccagatgcc agaatcccca
    26101 ctgcatggcg gcgtctttta ccgttgtgct ttgcgaccaa gggcttatca gttcgcggcg
    26161 tacggcgttc aggcgaatgc gtttcagcca cgcgtttggg ccaatgccta aaatagcgtg
    26221 aaacgcgttt tgtagcgtgc ggcggctgac atgcagttga ttacacaagt ccagcaccgt
    26281 cactggttcg gacatgtttt ccagcacata ttcacgggcg cgggaaagca atcgacggta
    26341 actctgatga ctgatgcttt ccgccgtcac cattggctgc gcttcttcca gcatcgcccc
    26401 catcgccatt agcaaattat cccccagcac ttttcgcact gcaggctgat ggagattttc
    26461 cgggttttcg caaaacgtcg ccagcgcctg ttggacaaag ccccacagag cggctttatg
    26521 ctgctctttc acttccaacg ccgactggct acgcagcata tgtaataccc gatccggatt
    26581 atgcaaaaag ttagcctgac gagtgatgac atcttcagaa agcactacgc caaggatcgt
    26641 gtaatcgtcc ggcgtactta attcaaactc ggttccaccg gggcgcgtcg ctatttctgc
    26701 gcttcccaga cattgcgaac cgataaatcc ctgctcaccg cgcgtcgccg gaatgccaaa
    26761 ccagaacgag ttcggccaga ccaggcacga ctggcgcagc gccagaccgg tgtattcacg
    26821 gaaaacctga atatcatcga gtagaatttc cgtaaattca ccatgaaact tgcccggatg
    26881 cagctgatcg taaatctgct gccaggcggt aattgtcaaa gcatgttcat agacatccgt
    26941 tgtctgtcgt tgatgaacat tatccacttc gaccttcggc gtgagcttca ggttttcggg
    27001 taagggttca tgataaagat ggtgcaaatt ggctgtacgg gtctttttca tgatgttaat
    27061 gccgggtgtt gtaggacacc cggcacctcc gacaggttaa tggggcttga gacgataacg
    27121 actactgcgt ttacgtaacg tcccggcaga aaagagctgt tcgagcgcat ttctggcttt
    27181 ttccagcggc cagccaaagt gggcagccac ttctcctgcc gtcattccct gacgtactga
    27241 tgttaacagc gccaacaact cgtcagcaga ttccgcgacg ataactggcg cgtcgggttc
    27301 ctttacgggt tgcttaggct ggcggttaaa gccagtaacc agccactggg tgtcatcgtc
    27361 tggccgaccg atctccttac ggctgatgac acgcccggta cgcatcgcgg cagcacaacc
    27421 tgcgtccagc gccgcacgac acgccgccag atcgccttcc accaccagcg ttaggcgacc
    27481 agggtcaagc acttcgtgac tgagcagacg aacgttagct gctttgagca tggcatcagc
    27541 tgcatctatc gcggcgacca tgccgtccac ttccagcaat cccagtgcat tgatcatcgg
    27601 caacctccgt tacgcacgct ggattggatt acgcgcgatt tccagcactg catcggtaaa
    27661 ggcgttacag gctgctttac acgcggcctg gctaccggtt aaaaatgctg ccgagtagtt
    27721 ggtttcagac ggtggcggga cataggtcac cagctgtacg tcggcagatt tcagggctgc
    27781 atcaatgccg taggtcgctt ccagcggcgg tgccaccaga tacgccatcg gatcgccaag
    27841 cgtgatcccg gcggttgatg agagataaga accggtacgc gaaactacat gtgccaggaa
    27901 ggctgtgttt tctgcgtcat tagcccactg gaaagcagcg ccattttcaa tatgcgcaac
    27961 catcgcatcc agaccagcac gcacttccgc cgggtttggc ccaccaagca taatcagcac
    28021 ttcaccggca gtcggtgacg ggccgtgtgc ggctcccgca tacagcgagc ggccatacac
    28081 cacttccacc atcgcctgct tggtcgcttc gtcagccgca atgtaggtga cgtcatctga
    28141 atcagcagaa atcagcccga ggctacgaat atgcggcggc aatttcagtt cacgcgcaaa
    28201 ttcggcgtta acggaggcaa tcacccgcat ggcggttacc gacggtcgaa tcaaatctaa
    28261 agctggcatg atgcctcctt atcgggtcat gttgatgccg gatgctttct gctccagcat
    28321 acgtttcgcc aaatccacaa tgacggctgc ggcttcaacc ggcggcgtgc cgccctggtg
    28381 aatgttagag atacaggtac ggtcggcctc aacggtggtt gccatacgcg gcgagtaaac
    28441 ggcgtagcag gagaggcttt ctgactgccc taatcccggg cgttccccca ccagtaggat
    28501 cactactttc gcgccgagga tctcaccgat ctgatcttca atcttcacgc gaccataacg
    28561 aacaaagaat ggtgtaccga ctttcagccc ggcctgtttc aggcccgcca tcagcggtgg
    28621 caggatctct tcgtagttca cggtgatcgc atcagttgac aggccatcag aaataacgac
    28681 ctgtacgtcc ggattagcaa cacactgtgc tttcagcgct tcaacagctt ctgcacacag
    28741 gcggcggccc atatccggac gagtcaggta gagatttttg tcgctaattt cagagcggac
    28801 ttccagcaag ccctgcgctt tcacccactc ttccggtact tctttcagta cggtatcttt
    28861 cgaacgagag tgatcggcca ggaaacgcag caacgcctgg gtacgcggac gcggaccggc
    28921 acgaccggta caaacgcggg ccacggtgct gcgacgcagt tctgttaata cgtctgcgcg
    28981 atgcgggttt tccacaccaa tccacgcttt tgcttccgca gaacctaaat ccagcgcgca
    29041 gctttccgag gtcactggtg tcgtacaggt ggtggtggcg cactttgctt ctgacggcgc
    29101 gggggccgct tgtcccattg acgccatcac gctgcgtaca atttcttcaa tctgtttttg
    29161 atccatgatg tgttatctcc gcgtcatcag aagaacagtg acggatcgcc cgcccgtttg
    29221 gtcaggcgac cgtttgccat aatgcccatg ctttccagcc agcgttcaaa ctccggtgac
    29281 gggcgcaggt tgagtaactg acgcacagtg gcggtgtcgt ggaaggccgt agtctggtag
    29341 ttgagcatga tgtcatcacc cagcggcatc cccatgatgt agttacagcc tgcggtggcg
    29401 agcaggatca tcaggttttc gttgaggttc tggtcagcgt cagcgtggtt ggtgtagcag
    29461 cagtcacagc ccatagagat gccgctcagc ttgcccataa agtgatcttc taagcccgcg
    29521 cggataatct ggcggtcgtt gtagagatac tccggcccaa taaaaccgac cacggtgttg
    29581 acgataaacg gatcgtaatg acgcgccagc ccatagttac gcgcttccat cgtcacctgg
    29641 tcagcgccga agttagcgcc agcggacagc gccgaacctt gtccggtttc gaagtagagg
    29701 cagttttccc cggcgatacg gttaaactct gcacccactg cgcgcgcttc gtcgagcatc
    29761 gccagctcca cgccaaactc tttcagccct ttttcgctgc cgcaaatact ctggaaaatc
    29821 agcccgcccg gtgcgccgcg acgaatcgct tcgatctggg tggtgacgtg cgccagtacg
    29881 cagccctgag ttgggatgtt gaatttgtcg atcacgccat agatggtatc caacacgcgg
    29941 cttaagtttt ccacgtcgtc agtcaccggg ttaacgccga tcaccgcatc gcccaccccg
    30001 aaggaaagcc cttcgtagat ttgcgcggcg atactttgca catcgtcacg ggtatcgttt
    30061 ggctgcaagc gggcgctaaa ggtgcccgga ataccgatgg tggtattggc ctttttgatc
    30121 accggcattt tcttcgcgcc gtagatcagg tccgcgttgg agcaaatctt cgctaccgcc
    30181 gctaccactt ccgaggtcag ccctttgcgg gtaaaggcaa tgtcgtccac gctggtttca
    30241 tcgctcagca catactcacg cagttcgctg atgctccagt ttttaatctg gttgtaggcc
    30301 gtttcgttaa catcgtcctg aatcagccgc gtcacgcagt catcttcata ggcaatcacc
    30361 ggattattgc ggatgtccgc tacggtcatt tccgacaaca cctgctttgc cgccacgcgc
    30421 tcctgtgagc ttgccgcagc aacgcccgcc agcacatccc ccgaacgcag ttcgttggct
    30481 ttagccagca cctcttttac atccttaaac tgatatacat tgccgaacaa tgtggtcttt
    30541 agtttcataa gtcgttccct caggaaggaa atgcgagtga tttcaccgtc accggcacaa
    30601 ccgatccgcc aaaaagaggc gtaccaatgt cgatatagtc ccccgcgcgg acaatcactt
    30661 cgtcaatgac tgccaacggg agttgttgta gctgtgggcg caacaacatg cccagagctt
    30721 taccaaagtc ctgcccggcc accaccagca ggggatgcgg attcggaaaa cgcgcgacga
    30781 aatcgaccaa cgcgttgatg accgtcagta ccgcggcgta acgcacaggc agcgaggcgg
    30841 gaagcgccag cacgtacgcg tcagttttag gatcaagatc cagctgaatc agcgcctgtt
    30901 gccaggcact caccagatcc ttttcatcaa ttgggatcgc caccggcaag ttgcgcagcg
    30961 gcagttgtac gccctccagc cagattgtgc tgccagagag cgaaagggta tgtgcacccg
    31021 cgccaatcac cgtggcgcgc acggtttgcg ccggaaactg cacgttcatc tcacgcaggc
    31081 gcggatggtc atgcagcgcc gttgccagaa gcgggccaat atcggcaaaa cagaacgggt
    31141 cggcgggctg gtggcgataa cattcgccaa cgccgccaga aagcgtaatg atttcgggcg
    31201 taacacctac gggcagcaaa ccggtttgca tcaatgcctg cgcgagcggc gaaagcgttc
    31261 cgtcaatcac ttcgacaatc agctctgcca tccgccgggt aacctgcacc agctgcgcgc
    31321 cggtcagcga acgggcttcg gtgcctgcac cgaagcactc atccacaatc atctgccccg
    31381 gtttatgagc gtaaaccaca cgcccctggc tgtcggtttc cagcaggcga ccgccgacat
    31441 tgaggcaggc ggtgccgctg atttttccgg catcgaacag ggcgtagttc gcggtgccac
    31501 cgccgatatc gatattcagg acccgacaca gccgttgttc agaaagggtt tgcgccccgg
    31561 caccgtgacc ggcgatcacg gattcgagat gcggtccagc gctggcgacg acaaaatcgc
    31621 ccagcgactg ggagagcgtc atcaccgccg ggcgagcgtt gcgggttttc gcgctttcac
    31681 cggtgatgat gatggcacca gaatcaacgc tttccggcgc aatgcccgca gcctgatatt
    31741 gctcgaggat taaggttttc agttccgctt cttttaaacc accctgttta tcgacagggg
    31801 taaagaacac cgggctttgc cagctaattt cgcgtttgat gaattcgtag cgcggcacct
    31861 gcgacaccgc cgcacggtta accagctcca ggtgggagaa aatcacctgg gtggtggtgg
    31921 tgccgatatc gataccgacg ctcagtagct ggcgagtgtt cacgattgcg cctccgcttc
    31981 ggttttagcc gcgctcgcgt cttctttagg taccagcatc atcgccacgc caatcgccgt
    32041 gacgccgccg atcagcttgc cgacaatcat cgggaagatc atggcgttca tgttggcagc
    32101 cgcgaagcct aaatggtcgc ccagggcgaa agcagcggaa acggcgaagg cacagttgat
    32161 gactttgccg cgggtatcca tctgcttcat catgccaaac atcgggatgt tgttggcaag
    32221 cgttgccacc atgccggctg ccgctatatt gttcatattc agcaccttac cgacactcat
    32281 cagcggtttt tcaaaccagc gagtcagcag cagcaccatc ggatacgccc ccagcagcac
    32341 gcaggagata gaaccgataa cttcaatggc gcgcatcact tcaccgggtt tgtcgcctgg
    32401 agccatgaag atcggatcca gaccggggat cagttcccaa ccgagcagga atttcactac
    32461 cgcagcggca agaccgaggg tgatcaatgc aacgaggaat ttggcgaaga tctggaagcc
    32521 gttgatcatt ttttccggga tgaatttcag ccccagcgcc accagcaccg caacgataag
    32581 taccgggatc atgttcatca ggatcagcgc aaaggtgaat tccaccggct gaccgttgat
    32641 ctgcacaccg gagtacatag caaccagacc accagcaata cagccaatcg gaatggtcac
    32701 aatgcccgcc agcacgccga gcgccagata acgacggtca gaaggttcga taatgccgag
    32761 cgccaccgga atggaaaaca caatcgttgg ccccatcatc gacccgagaa ttaacccaga
    32821 gtatagccac gctgctacgt cgccgcccgc cagctctttg gcgaggaaga agccgcccat
    32881 atcgcacgcc agcagtgttc cggcgaacat cgatgggtta gcgccgagca tttcgtaaac
    32941 cgggataatc accggcccga gtacgtgggc cagtaccggt gccagcgcgg tcataccgac
    33001 catagccagg cccaaagcgc ccattgccat aaagccttct tcgaactgac cgccggatcc
    33061 ttcgatactt ttaccgaact taccgaggaa acgagcagaa ccgccgaact gcgacaggat
    33121 cctgtctacg gcagctatca gcataaagaa catcatgatg tacatgatga tttcgttaat
    33181 acccatcgcc tttactccct gttagttgtt atttattggc ggatgcggcg tgaacgcctt
    33241 atccgcccta catgtgcaat cccgaaggcc agataagacg cggcaagcgt cgcatccggc
    33301 aattgcaccg cgccactggc ggatgcggcg tgaacgcctt atccgcccta catgtgcaat
    33361 cccgtaggcc ggataagacg cggcaagcgt cgcatccggc atttattgcg ccgctgcgta
    33421 caggccgaca atctgctcca ggctggcggt acgcgggtta ctgcgcagac aaatatcttc
    33481 cagcgcggcc tgcgcccatg cgccgtaatg cgcagatgtc gcaccaacat cgcccagtcg
    33541 tttaccaatc ccaacttccg ctatcagctc acttaccgca ttaatagcgt cacgatcgtc
    33601 ggatttttta gttcgcagcg cccgaccaac caagctaaac cgatcacgac aaaccatccg
    33661 gttaaattcc atcaccgttg gcaacaacat ggcgttcgcc agaccgtgcg gaatatgcag
    33721 cgccgcccct ggctgatgcg ccatcgcgtg gcacaaccca agtccggcac tggaaaacgc
    33781 cattcccgcc atacatgaag ccagcaacat gctctcgcgc gcggcaaggt catgaccgta
    33841 gcccaccgct ttcggcagcg atttgccaat catcgcaatg gcaccaatcg ccagactgtc
    33901 ggtaaacggt gtggcgttca gcgcgctgta cgcttcaatg gcatgggtta acgcatcaat
    33961 gccagtcatc gccgtgacat gcgacggcac accttcggtc aatgcggcgt cgagaatcgc
    34021 cacatccggc atcagcgagg catgggctaa cacctgcttg cgcccgctca ccgcgtcgat
    34081 aatcaccgtt acgttggtgg tttcagagcc ggttccggcg gtcgttggaa tggcaatcaa
    34141 cggcaggcgc ggttgcagaa cgctggtttc tgacatttct gccagcgtgc tatcggggtt
    34201 cgtcaccagc aacgccacgg ctttcgccgc atccagcacc gagccgccgc caaacgcgat
    34261 caccccgtca caacctgatt cacgcaactg cgccacggct gcacacacgt cggtgatgca
    34321 cggttcgccc accggacatg gccagagcgt catggcgata cctttaacgg ccaggctacg
    34381 cgtcagcccg gcggtcatcc ccgcctgatg caaaaagctg tctgccatca cgaacagatg
    34441 tttcagccca cgcgtttgcg cttgctgccc acaactgctc accgcgcccg gaccgcaaag
    34501 cgtcaccggt ggaacgctaa atgttttcac ccgctgcaga ttcagggtat cgaatgcctg
    34561 aaaaagcgcc gtttgcaatt cactttgcat agatcccctc cgttttctct cgcccactac
    34621 tggcgatcgc cagcggggtc ataaacaaac tgtgctgcgg taaatgcacc tgtaacgccg
    34681 ggaattgttt gcgaaacagt tccgctacgc ccggttgcat acaggaaccg cccgccagcc
    34741 ataaatccgt aatgccctgc ccttcaatat gccgggccac aatgtccgcc attttttcgt
    34801 acaccggttt caccgcgggc caaatctcgt caccgtgacc gcgtttgtac tgctctgcct
    34861 cttccagcga aatacggcga tttccggcga gagtcagaga gatgtgatgt ccgccggtgg
    34921 cttcatccgc cgagtacgtc actttgccct ttttcacgat ggcaatgccg gtagtgccgc
    34981 cgccgatatc cactacaccg gcgttgtcca gttgcagcag atccgcaact gcggttggct
    35041 catccagcac atggctcact tccagtccag cagactccag cacgttgatg gaaatacgcg
    35101 ggtccgtacc tggaggaaaa gaggtcgccg catggctaaa acggcgaccg aattgctgct
    35161 cgagggtgtc gagatggcga cgaacaatgg tgaccgcacc gaagaaatcc cagacgatgc
    35221 cgtcgcggac cacatcagcc cagtcgaggc ataccgccac cggctgaccg tcgcggtcga
    35281 caaccatcga caccacatcg caggtgccca gatccacccc cagccacagc ggagattccg
    35341 tcgcggcggg cgtctggtta cacagcgttg ccgccgtttg cagacgtggg gtgagccatt
    35401 gttcgtcgtg cgccatctgt tactccttaa acaatgcgaa acgcatcgac taatacacag
    35461 cgacgcagac ggacaaacgt ccgcgcgctg gttacccctt caccggttgg cgtggtgatg
    35521 gtcatagtgg tccagccttc cccgcccagc cccagcccgg caatgcacgg tccgttctta
    35581 acgaaaatgc tggtatcaat ggcattcgcc atctggttca tgttctcgat attgcgcgag
    35641 tgcattgccg ccgtgtggtg gcaaccgcct tccagtttca ccgccagcgc aatggcatcc
    35701 gccacgttgg cgacgcgcac gacgggcagc accggcatca tcagttcagt cacagcaaac
    35761 ggatgttctg cggtggtttc tacaaacagc agtcgcgttt gttccggaac tttaaggccg
    35821 attgccgccg cgattttcgc cgcatcgcga ccaacccagt cacggctgac ggtgcctttt
    35881 ccgcgctcgt cgatattttt cagcaacacc gattgcagtt gctgcgcctg ctctgcggtc
    35941 agtttcaccg catgctggcc ttccatcaga cgcatcagtt catcggctac gctatcaaca
    36001 acaatcagta ccttttcgtc ggcacaaatg atgttgttat cgaaagaagc acctttgacg
    36061 atggactgag ctgcacgggc gaggtcggcg gtttcatcca ccactaccgg cgggttaccg
    36121 gcacctgccg caatcaggcg tttattggtg tgtttgcgcg ccgcttccac taccgcttcg
    36181 ccgccggtca ccaccagcag gccgataccc ggaaacttga acaagcgttg cgcggtttcg
    36241 atatccggat ttgctacggt aaccagtaag ttttccggcc cgcctgcggc aacaatcgcc
    36301 tggttgagca gcgtaatcgc ccgctgggag acttttttcg ccgccggatg cggggcaaaa
    36361 atgacgctgt tgcccgcggc aatcaggctg atggcgttat taatgacggt tgccgccggg
    36421 ttagtggaag gcgtcaccga agccactacg ccccagggcg cgttttcaat taaagtcagg
    36481 ccgttgtcgc cagtcagcac ctgtggagag aggcactcaa cgcctggtgt gccgcgcgcc
    36541 tgagcgacgt ttttggcaaa tttatcttca acgcgcccca tgccggtttc actgacggca
    36601 agttccgcta aatctctggc gtgtttttcg cctgcttcac gaatggcagc aatggctaac
    36661 tggcgcattg ccacgctttt taacccttgc tgggcgactt tggctgccgc aacggcgtca
    36721 tccagggacg cgaaaacgcc catctcatga acggcggcgg gcggcgtgtc actgctttgc
    36781 attttcagca gtaccgcttt caccacctgt tcaatatcct gttgattcat gatgttctgc
    36841 cttatttgtg gaaaatgacc tgaccgccag acaccacctc atcgacgatg ccaatcacgc
    36901 acagatcgac cggtgacgtt tcgcttttat gcgcctggcg ggcggaactg ccactcacca
    36961 gcaacaccca ctccccggtt cccgcgccaa tattgtcgat ggcgacggcg cattgcccgt
    37021 caggattacc ttgtggatca atcatttcca ccatcagcaa tttgtcatgc gccagtccgt
    37081 gatggcgtac ggtacaaaca atttgtccag tgacgactgc cagtttcata cccgcctccg
    37141 tggcgtattt caggtaaaag ctccccctac cctccgcaga aggtaaaatg aaaaaggaga
    37201 gagcgtgacg cccgaatcga cgtcacacag ggtgattaca ggttgctgct atcgcctttc
    37261 aggccgatcg ggaagacttc ttccaggtcg ccgtgcggac gcgggataac gtgtacagat
    37321 accagctcgc cgatacgctg tgccgctgcg gcacctgcat cggtcgcggc tttacaggct
    37381 gcaacatcac cacgaaccat ggcagtacac aggccgccgc caatctgctt aacaccaacc
    37441 agcttgacgc gcgcagcttt aaccatcgcg tcagaagcct caatcagtgc aaccaggccc
    37501 cgggtttcga tcattcctaa tgcttccatt gtgctttcct ctttatcagg gtccagaacg
    37561 ggaccgttca ttcaaccagt gtttgtaaac tgctttcgcg gttcacttct gtctgacgct
    37621 gcacagctgc caccagcgcc agttcgataa tttcctgcac gctacaacca cgagagagat
    37681 cgtgcatcgg cgcggcaagt ccttgtatca atggcccgac ggcacgatat ccgccaagtc
    37741 gttgtgcgat tttgtagcca atatttccgg cttccagcga cggaaaaacc atcacattag
    37801 ctttgccctg taacgggctg gcaggcgctt tttgcgccgc cacttccggc acgaaggcgg
    37861 cgtcaaactg caactcgcca tcgacaacca gctttggtgc gcgctcacgg acgatttctg
    37921 tcgcctgctg gacgttggca acgcaggggt gacgggcact accgttgctg gaaaacgaca
    37981 acatcgccac gcgcggttct tctccggtga tggcgcgcca ggtttcggcg ctggcaagcg
    38041 cgatatccgc cagttgcgcc gccgtcggct gtggcactac gctgcaatcg gcaaagccca
    38101 atgcggggcc actgtactgt ggcagcatca ggaaaatgga ggagagcgtt ttacagcctg
    38161 gctgtaagcc gataatgcgt aatccggcac gcagcacatt cgccgtggaa gagagattgc
    38221 ccgcgataca gacatccgct gtcccggcgc tgaccattgc ggcagcgaac atcagcggat
    38281 cggtgagttt atccagcgca tccggcggcg ttttttcgcc cgcacgggcc agccagcgga
    38341 gagcaaattc ttcccgcatt gcgaggttgc catgcggatc aatcacctgt agcccgtcca
    38401 tcgccacgcc gtgactgagc gcaaactgac gaagttcaaa cggattggcg accagaatgg
    38461 gcgttgccag accttgttga tgtaaatatt gcgccgcttt cagcacacgt tgatctaacg
    38521 catccggaaa aactactctg gcgggcgctc gcaacgccag tttacgacaa cgttcaataa
    38581 tcattgcgtt tcccccagtc gttgttggat ctgcttcacc gttagcgact gcttcgttac
    38641 gctcaggatc atcatcacgt aaaccgtgct ggaaaggcgg ttcagcgcct gcaagatgtc
    38701 cgggcgtaat acttcaaagc tgcgggtgat aaacacctgc gccgctacag tttcggtttc
    38761 ccgcactttg gtgcgtagca gattgagcaa cgccgcatca cgcccgtggc tggcttccgg
    38821 caccagatga tcgtggtcga gatagcgcag cggctggtga gaaagccgat gcagatcttc
    38881 gtcagaaagc ccgacaatcg cttgttcgcc cagcggttcc cccagcgcat cggcgcgcat
    38941 aatgttgcca agacgcgaac ggatatccgc cagccacggc tgccacggtt ccgccagttc
    39001 aatttgcagc cacactgcca gcgcaatggt gctgtcgaga acagcgcgaa aagccaggcg
    39061 cggatcgctt ttggcaacca ttttttccgc cgacaggtga gtcagcgtat ccggcttttt
    39121 tgccaccggc tggcggcaca gttcgcagca cgcctgcgga tgttcatcgc tactggttag
    39181 cccgtgaacg ggctgcggct gctgctgttc atcgtcaaca aacaggcggc cctgctcgtc
    39241 aataaacttg atgcgcagat ggcggctttc cagtaactcc cgggcagagg gcgtcaggcg
    39301 actgtccgca gggagatgga tctctgcgcc ttcgctgagc gtatgattcg ctcttagcca
    39361 tgcttcggtg atgaaatctt tcataaggat tgccagttag ccggccaggc gacatacagg
    39421 aatttcacgc tggatgtcgt accaaattcg atgctggagc ctttcgggat aaacatcaca
    39481 tcgcccgctt tggcgatcat agtttcgcct tcgtggcgaa catgcagctc cccttccagc
    39541 accatgtcga tttcgtcgta gttcagggtc cacggaaaga aggcgttctc ccactgcatg
    39601 aaaccagcgg ccatactgct gccgtcgtct ccggtcacga gatcagtcaa gcctacgcag
    39661 tgcggttccg cgccatcgaa acgaccaaac ttgacgctgc tgccatcaat aactttgatg
    39721 ccgcctttgc cggtcaccga tttaaagctc ggctgcagtg caccctgctc cagcgactgt
    39781 ttttccttca tcactttttc catcagctgc gcgaccaggc tttcggtaaa ctgaccttcc
    39841 ggcagttggg cgataatggt ttcacggatg cgctggcttt cggttttatc ggctgatgcg
    39901 ctggcgggaa cagacgccgt taccgggatc gattcatcac attcggtgat ggtaaaaccc
    39961 aacagatcag ccacttcacg agcttccggg gtaataatgc tggcgcgcag aaccacggac
    40021 attgccagtt cgccgcgcgc gtgtgcttca cgaatatcat tcgctgtgat gagttttttc
    40081 acctgcttcc tcctgtttgg tgagagatgc cagataatcc accagttgct gcacgctttg
    40141 cgggtcgtgg ctgtttagct cgaaaatcgg ctcttcgaag cccgtctcaa gcaataactt
    40201 tcgcgttgcg gcgacgtcag catctggcat gtccgtttta ctgatgacgg cgatttgccg
    40261 tttgctgacg ccaatatcca gcaacccggc aggcaaccga ctttccgggt cattcgcgcc
    40321 gtggacatag atcagcatgt ccacatcctg tagcgtggta attaatgcgt gataccagcg
    40381 gggatggcta aaatattcac ccggcgtatc aatatcgcca ttatcattaa attccacggc
    40441 ctgcgttttt ctggcgaggg tataattccc ctgcaacgca ttaaataacg ttgtttttcc
    40501 tgccccgacg gtacccacaa acgcaattcg tttcatgaat gcctcatatt aacttttggt
    40561 catttcacaa agcgtataat ttaataagcg tcctaaacca ctgacggttt gtgataacgc
    40621 ttcttctact gcgcccaccg aaccataaat caccagcgcg ccgctaaaac ggtcgagaaa
    40681 accgatatgc acatcggcgg ctttcaacgc taaatcaccg gcgatcatcg ccgtttctcc
    40741 gggagttagc gtcataatgc caattgcgcc cgcatccgga acgccgattt tcttcgccag
    40801 ttcctcgccg ggatgcgcga tgagatgcgc cagcgtgacc tgtttgccgg gcacaaattc
    40861 ctgaatgatg cgttctttat ccatgagtcg ccgcctgtga aaaactttcg tgtatcgtga
    40921 caatttttcg tcagagtaaa taacaaaatt cggcaagcgc gtttaaaagg tgaggtagat
    40981 cactaagaat ggagagatat ttactgttat
//
LOCUS       DAZFLD010000013        38663 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.013, whole genome shotgun sequence.
ACCESSION   DAZFLD010000013 DAZFLD010000000
VERSION     DAZFLD010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 38663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38663
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.013"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            174..1829
                     /gene="aslA"
                     /locus_tag="ACHKBL_000637"
     CDS             174..1829
                     /gene="aslA"
                     /locus_tag="ACHKBL_000637"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase AslA"
                     /protein_id="HFO5817559.1"
                     /translation="MEFSFSPKLLVVAVAAALPLMANAADTPSTATARKGFAGYDHPN
                     QYLVKPATTIADNMMPVMQHPAQDKETQQKLAELEKKTGKKPNVVVFLLDDVGWMDVG
                     FNGGGVAVGNPTPDIDAVASQGLILTSAYSQPSSSPTRATILTGQYSIHHGILMPPMY
                     GQPGGLQGLTTLPQLLHDQGYVTQAIGKWHMGENKESQPQNVGFDDFRGFNSVSDMYT
                     EWRDVHVNPEVALSPDRSEYIQKLPFSKDDVHAVRGGEQEAIADITPKYMEDLDQRWM
                     EYGVKFLDKMAKSDKPFFLYYGTRGCHFDNYPNAKYAGSSPARTSYGDCMVEMNDIFA
                     NLYKALEKNGQLDNTLIVFTSDNGPEAEVPPHGRTPFRGAKGSTWEGGVRVPTFVYWK
                     GMIQPRKSDGIVDLADLFPTALDLAGHPGAKVANLVPKTTFIDGVDQTSFFLGTNGQS
                     NRKAEHYFLNGKLSAVRMDEFKYHVLIQQPYAYTQSGYQGGFTGTVMQTAGSSVFNLY
                     TDPQESDSIGVRHIPMGVPLQTEMHAYMEILKKYPPRAQIKSD"
     gene            complement(1987..3222)
                     /gene="aslB"
                     /locus_tag="ACHKBL_000638"
     CDS             complement(1987..3222)
                     /gene="aslB"
                     /locus_tag="ACHKBL_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HFO5817560.1"
                     /translation="MLQQVPTRAFHVMAKPSGSDCNLNCDYCFYLEKQSLYREKPVTH
                     MDDDTLEAYVRHYIAASETQNEVAFTWQGGEPTLLGLDFYRRAVALQAKYGAGRKISN
                     SFQTNGVLLDDEWCAFLAENHFLVGLSLDGPAEIHNQYRVTKGGRPTHKLVMRALTLL
                     QKHHVDYNVLVCVNRTSALQPLQVYDFLCDAGVEFIQFIPVVERLADETAAHAGLKLH
                     APGDIQGELTEWSVCPQEFGEFLVAIFDHWIKRDVGKVFVMNIEWAFANFVGAPGAVC
                     HHQPTCGRSVIVEHNGDVYACDHYVYPQYRLGNMLQQTIAEMIDSPQQQAFGEDKFKQ
                     LPAQCRSCNVLKACWGGCPKHRFMLDASGKPGLNYLCAGYQRYFRHLPPYLKAMADLL
                     AHGRPASDIMQAHLMVVNK"
     gene            complement(3369..3445)
                     /locus_tag="ACHKBL_000639"
     tRNA            complement(3369..3445)
                     /locus_tag="ACHKBL_000639"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3409..3411),aa:Pro,seq:tgg)
     gene            complement(3488..3574)
                     /locus_tag="ACHKBL_000640"
     tRNA            complement(3488..3574)
                     /locus_tag="ACHKBL_000640"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3538..3540),aa:Leu,seq:cag)
     gene            complement(3595..3670)
                     /locus_tag="ACHKBL_000641"
     tRNA            complement(3595..3670)
                     /locus_tag="ACHKBL_000641"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3635..3637),aa:His,seq:gtg)
     gene            complement(3729..3805)
                     /locus_tag="ACHKBL_000642"
     tRNA            complement(3729..3805)
                     /locus_tag="ACHKBL_000642"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3769..3771),aa:Arg,seq:ccg)
     gene            complement(3908..5293)
                     /gene="yifK"
                     /locus_tag="ACHKBL_000643"
     CDS             complement(3908..5293)
                     /gene="yifK"
                     /locus_tag="ACHKBL_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFO5817561.1"
                     /translation="MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPS
                     VLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWSYWFMWMA
                     VGISEITAIGVYVQFWFPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTT
                     IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVEL
                     IGITAGEAKNPQVTLRSAVGKVLWRILIFYVGAIFVIVTIFPWNEIGSNGSPFVLTFA
                     KIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLPTAMAKVSRHGVPVA
                     GVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAA
                     IASHPFRSILFPWANYVTMAFLICVLIGMYFNEDTRMSLFVGIIFMLTVTAIYKVFGL
                     NRHGKAHKLEK"
     gene            complement(5484..6224)
                     /gene="wecG"
                     /locus_tag="ACHKBL_000644"
     CDS             complement(5484..6224)
                     /gene="wecG"
                     /locus_tag="ACHKBL_000644"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047241 - lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HFO5817562.1"
                     /translation="MNNNTTAPTYTLRGLQLIGWRDMQHALDYLFADGQLKQGTLVAI
                     NAEKMLTIEDNAEVRELINAAEFKYADGISVVRSVRKKYPQAQVSRVAGADLWEELMA
                     RAGKEGTPVFLVGGKPEVLAQTEAKLRNQWNVNIVGSQDGYFKPEQRQALFERIHASG
                     AQIVTVAMGSPKQEIFMRDCRLVHPDALYMGVGGTYDVFTGHVKRAPKIWQTLGLEWL
                     YRLLSQPSRIKRQLRLLRYLRWHYTGNL"
     gene            complement(6227..7579)
                     /gene="wzyE"
                     /locus_tag="ACHKBL_000645"
     CDS             complement(6227..7579)
                     /gene="wzyE"
                     /locus_tag="ACHKBL_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECA oligosaccharide polymerase"
                     /protein_id="HFO5817563.1"
                     /translation="MSLLQFSGLFVVWLLCTLFIATLTWFEFRRVRFNFNVFFSLLFL
                     LTFFFGFPLTSVLVFRFDVGVAPPEILLQALLSAGCFYAVYYVTYKTRLRKRVADAPR
                     RPLFTMNRVETNLTWVILMGIALVSVGIFFMHNGFLLFRLNSYSQIFSSEVSGVALKR
                     FFYFFIPAMLVVYFLRQDSKAWLFFLVSTVAFGLLTYMIVGGTRANIIIAFAIFLFIG
                     IIRGWISLWMLAAAGVLGIVGMFWLALKRYGMNVSGDEAFYTFLYLTRDTFSPWENLA
                     LLLQNYDNIDFQGLAPIVRDFYVFIPSWLWPGRPSMVLNSANYFTWEVLNNHSGLAIS
                     PTLIGSLVVMGGALFIPLGAIVVGLIIKWFDWLYELGNRETNRYKAAILHSFCFGAIF
                     NMIVLAREGLDSFVSRVVFFIVVFGACLMIAKLLYWLFESAGLIHKRTKSSLRTQVEG
                     "
     gene            complement(7576..8655)
                     /gene="rffT"
                     /locus_tag="ACHKBL_000646"
                     /gene_synonym="wecF"
     CDS             complement(7576..8655)
                     /gene="rffT"
                     /locus_tag="ACHKBL_000646"
                     /gene_synonym="wecF"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HFO5817564.1"
                     /translation="MTVLIHVLGSDIPHHNRTVLRFFNDALAATSEHAREFMVVGKDD
                     GLSDSCLALSVQFFPGKKSLAEAVIAKAKANRQQRFFFHGQFNPTLWLALLSGGIKPS
                     QFYWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGCVFATRGDLSFFAKTHPKVRGEL
                     LYFPTRMDSSLNTMVNDRQREVKMTILVGNSGDRSNEHIAALRAVHQQFGDTVKVVVP
                     MGYPPNNEAYIEEVRQAGLELFSEENLQVLSEKLEFDAYLTLLRQCDLGYFIFARQQG
                     IGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDIVREAQRQLASVDKN
                     TIAFFSPNYLQGWQRALAIAAGEVA"
     gene            complement(8652..9902)
                     /gene="wzxE"
                     /locus_tag="ACHKBL_000647"
     CDS             complement(8652..9902)
                     /gene="wzxE"
                     /locus_tag="ACHKBL_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid III flippase WzxE"
                     /protein_id="HFO5817565.1"
                     /translation="MSLAKASLWTAASTLVKIGAGLLVGKLLAVSFGPAGLGLAANFR
                     QLITVLGVLAGAGIFNGVTKYVAQYHDNPQQLRRVVGTSSAMVLGFSTLMALVFVLAA
                     APISQGLFGNTDYQELVRLVALVQMGIAWGNLLLALMKGFRDAAGNALSLIVGSLIGV
                     LAYYVSYRLGGYEGALLGLALIPALVVIPAAIMLIKRGVIPLSYLKPSWDNGLAGQLS
                     KFTLMALITSVTLPVAYIMMRKLLAAQYSWDEVGIWQGVSSISDAYLQFITASFSVYL
                     LPTLSRLTEKRDITREVVKSLKFVLPAVAAASFTVWLLRDFAIWLLLSNKFTAMRDLF
                     AWQLVGDVLKVGAYVFGYLVIAKASLRFYILAEVSQFTLLMVFAHWLIPAHGALGAAQ
                     AYMATYIVYFSLCCGVFLLWRRRA"
     gene            complement(9904..11034)
                     /gene="rffA"
                     /locus_tag="ACHKBL_000648"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(9904..11034)
                     /gene="rffA"
                     /locus_tag="ACHKBL_000648"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019180 -
                     dTDP-4-amino-4,6-dideoxygalactose transaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HFO5817566.1"
                     /translation="MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRF
                     GSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRP
                     DTMNIDETLIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKYNLFVVEDAAQGVMST
                     YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFR
                     GQVDKYTWRDIGSSYLMSDLQAAYLWAQLEAADRINQQRLALWQNYYDALAPLAKAGR
                     IELPSIPDGCVQNAHMFYIKLRDIDDRSALINFLKEAEIMAVFHYIPLHGCPAGERFG
                     EFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS"
     gene            complement(11039..11713)
                     /gene="rffC"
                     /locus_tag="ACHKBL_000649"
                     /gene_synonym="wecD"
     CDS             complement(11039..11713)
                     /gene="rffC"
                     /locus_tag="ACHKBL_000649"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HFO5817567.1"
                     /translation="MPVRASIEPLTWENAFFGVNSAIVRISPEAPLLTPDALAPWSRV
                     QAKIAASNTDELDALQQLGFSLVEGEVDLALPVNNVSDSGAVVAQETDIPALRQLASA
                     AFAQSRFRAPWYAPDASGRFYAQWIENAVRGTFDHQCLILRSASGDIRGYVSLRELNA
                     TDARIGLLAGRGAGAELMQTALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVEST
                     AYWLYR"
     gene            complement(11691..12572)
                     /gene="rfbA"
                     /locus_tag="ACHKBL_000650"
     CDS             complement(11691..12572)
                     /gene="rfbA"
                     /locus_tag="ACHKBL_000650"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFO5817568.1"
                     /translation="MKGIILAGGSGTRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLA
                     GIREILIITTPEDKCYFQRLLGDGSEFGIQLEYAEQPSPDGLAQAFIIGETFLNGEPS
                     CLVLGDNIFFGQGFSPKLRHVAARTQGATVFGYQVMDPERFGVVEFDDNFRAISLEEK
                     PKQPKSNWAVTGLYFYDSKVVEYAKQVKPSERGELEITSINQMYLEAGNLTVELLGRG
                     FAWLDTGTHDSLIEASTFVQTVEKRQGFKIACLEEIAWRNGWLDDEGVKRAASSLAKT
                     GYGQYLLELLRARPRQY"
     gene            complement(12591..13658)
                     /gene="rffG"
                     /locus_tag="ACHKBL_000651"
     CDS             complement(12591..13658)
                     /gene="rffG"
                     /locus_tag="ACHKBL_000651"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFO5817569.1"
                     /translation="MRKILITGGAGFIGSALVRYIINETSDAVVVVDKLTYAGNLMSL
                     APVAQSERFAFEKVDICDRAELARVFTEHQPDCVMHLAAESHVDRSIDGPAAFVETNI
                     VGTYTLLEAARAYWNALTEDKKSAFRFHHISTDEVYGDLHSTDDFFTETTPYAPSSPY
                     SVSKASSDHLVRAWLRTYGLPTLITNCSNNYGPYHFPEKLIPLMILNALAGKPLPVYG
                     NGQQIRDWLYVEDHARALYCVATTGKVGETYNIGGHNERKNLDVVETICELLEELAPN
                     KPHGVAHYRDLITFVADRPGHDLRYAIDASKIARELGWLPQETFESGMRKTVQWYLAN
                     ESWWKQVQDGSYQGERLGLKG"
     gene            complement(13658..14920)
                     /gene="wecC"
                     /locus_tag="ACHKBL_000652"
     CDS             complement(13658..14920)
                     /gene="wecC"
                     /locus_tag="ACHKBL_000652"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0089714 - UDP-N-acetyl-D-mannosamine
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HFO5817570.1"
                     /translation="MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINR
                     GEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAA
                     RSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGELADVNIAYCPER
                     VLPGQVMVELIKNDRVIGGMTPVCSARASELYKIFLEGECVVTNSRTAEMCKLTENSF
                     RDVNIAFANELSLICADQGINVWELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVA
                     QNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRASELKIACFGLAFKPNI
                     DDLRESPAMEIAELIAQWHSGETLVVEPNIHQLPKKLTGLCTLAQLDEALATADVLVM
                     LVDHSQFKVINGDNVHQQYVVDAKGVWR"
     gene            complement(14917..16047)
                     /gene="wecB"
                     /locus_tag="ACHKBL_000653"
     CDS             complement(14917..16047)
                     /gene="wecB"
                     /locus_tag="ACHKBL_000653"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-hydrolyzing UDP-N-acetylglucosamine
                     2-epimerase"
                     /protein_id="HFO5817571.1"
                     /translation="MKVLTVFGTRPEAIKMAPLVHALAKDPFFEAKVCVTAQHREMLD
                     QVLKLFSIVPDYDLNIMQPGQGLTEITCRILEGLKPILAEFKPDVVLVHGDTTTTLAT
                     SLAAFYQRIPVGHVEAGLRTGDLYSPWPEEANRTLTGHLAMYHFSPTETSRQNLLREN
                     VADSRIFITGNTVIDALLWVRDQVMSSDTLRSELAANYPFIDPNKKMILVTGHRRESF
                     GRGFEEICHALADIATTHQDIQIVYPVHLNPNVREPVNRILGHVKNVILIDPQEYLPF
                     VWLMNHAWLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVTAGTVRLVGTDKQRIVE
                     EVTRLLKDENEYQAMSRAHNPYGDGQACSRILEALKNNRISL"
     gene            complement(16103..17149)
                     /gene="wzzE"
                     /locus_tag="ACHKBL_000654"
     CDS             complement(16103..17149)
                     /gene="wzzE"
                     /locus_tag="ACHKBL_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECA polysaccharide chain length modulation
                     protein"
                     /protein_id="HFO5817572.1"
                     /translation="MTQPMPGKPAEDAENELDIRGLFRTLWAGKLWIIGMGLAFALIA
                     LAYTFFARQEWSSTAITDRPTVNMLGGYYSQQQFLRNLDVRSNMASADQPSVMDEAYK
                     EFVMQLASWDTRREFWLQTDYYKQRMVGNSKADAALLDEMINNIVFIPGDFTRAVNDS
                     VKLIAETAPDANNLLRQYVAFASQRAASHLNDELKGAWAARTIQMKAQVKRQEEVAKA
                     IYDRRMNSIEQALKIAEQHNISRSATDVPAEELPDSEMFLLGRPMLQARLENLQAVGP
                     AFDLDYDQNRAMLNTLNVGPTLDPRFQTYRYFRTPEEPVKRDSPRRAFLMIMWGIVGG
                     LIGAGVALTRRCSK"
     gene            complement(17161..18264)
                     /gene="wecA"
                     /locus_tag="ACHKBL_000655"
     CDS             complement(17161..18264)
                     /gene="wecA"
                     /locus_tag="ACHKBL_000655"
                     /EC_number="2.7.8.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0036380 -
                     UDP-N-acetylglucosamine-undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HFO5817573.1"
                     /translation="MNLLTVSTDLISIFLFTTLFLFFARKVAKKVGLVDKPNFRKRHQ
                     GLIPLVGGISVYAGICFTFGIVDYYIPHASLYLACAGVLVFIGALDDRFDISVKIRAT
                     IQAAVGIVMMVFGKLYLSSLGYIFGSWEMVLGPFGYFLTLFAVWAAINAFNMVDGIDG
                     LLGGLSCVSFAAIGMILWFDGQTSLAIWCFAMIAAILPYIMLNLGILGRRYKVFMGDA
                     GSTLIGFTVIWILLETTQGKTHPISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPD
                     RQHIHHLIMRAGFTSRQAFVLITLAAALLASIGVLAEYSHFVPEWVMLVLFLLAFFLY
                     GYCIKRAWKVARFIKRVKRRLRRNRGGSPNLTK"
     gene            complement(18504..19763)
                     /gene="rho"
                     /locus_tag="ACHKBL_000656"
     CDS             complement(18504..19763)
                     /gene="rho"
                     /locus_tag="ACHKBL_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HFO5817574.1"
                     /translation="MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAK
                     SGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIR
                     PPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTAR
                     VLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTE
                     MQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEE
                     FKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGE
                     IDAMEFLINKLAMTKTNDDFFEMMKRS"
     gene            complement(19848..19949)
                     /gene="rhoL"
                     /locus_tag="ACHKBL_000657"
     CDS             complement(19848..19949)
                     /gene="rhoL"
                     /locus_tag="ACHKBL_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rho operon leader peptide RhoL"
                     /protein_id="HFO5817575.1"
                     /translation="MRSEQISGSSLNPSCRFSSAYSPVTRQRKDMSR"
     gene            complement(20090..20419)
                     /gene="trxA"
                     /locus_tag="ACHKBL_000658"
     CDS             complement(20090..20419)
                     /gene="trxA"
                     /locus_tag="ACHKBL_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HFO5817576.1"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLA"
     gene            20550..21815
                     /gene="rhlB"
                     /locus_tag="ACHKBL_000659"
     CDS             20550..21815
                     /gene="rhlB"
                     /locus_tag="ACHKBL_000659"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HFO5817577.1"
                     /translation="MSKTHLTEQKFSDFALHPKVVEALEKKGFHNCTPIQALALPLTL
                     AGRDVAGQAQTGTGKTMAFLTSTFHYLLSHPAIADRKVNQPRALIMAPTRELAVQIHA
                     DAEPLAQATGLKLGLAYGGDGYDKQLKVLESGVDILIGTTGRLIDYAKQNHINLGAIQ
                     VVVLDEADRMYDLGFIKDIRWLFRRMPPANQRLNMLFSATLSYRVRELAFEQMNNAEY
                     IEVEPEQKTGHRIKEELFYPSNEEKMRLLQTLIEEEWPDRAIIFANTKHRCEEIWGHL
                     AADGHRVGLLTGDVAQKKRLRILDEFTRGDLDILVATDVAARGLHIPAVTHVFNYDLP
                     DDCEDYVHRIGRTGRAGASGHSISLACEEYALNLPAIETYIGHSIPVSKYNPDALMTD
                     LPKPLRLTRPRTGNGPRRTGAPRNRRRSG"
     gene            21949..23433
                     /gene="gppA"
                     /locus_tag="ACHKBL_000660"
     CDS             21949..23433
                     /gene="gppA"
                     /locus_tag="ACHKBL_000660"
                     /EC_number="3.6.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008894 -
                     guanosine-5'-triphosphate,3'-diphosphate diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0015974 - guanosine pentaphosphate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine-5'-triphosphate,3'-diphosphate
                     diphosphatase"
                     /protein_id="HFO5817578.1"
                     /translation="MGSTSSLYAAIDLGSNSFHMLVVREVAGSIQTLTRIKRKVRLAA
                     GLNSENALSNEAMERGWQCLRLFAERLQDIPPSQIRVVATATLRLAVNAGDFIAKAQE
                     ILGCPVQVISGEEEARLIYQGVAHTTGGADQRLVVDIGGASTELVTGTGAQTTSLFSL
                     SMGCVTWLERYFADRNLAQENFDAAEKAAREVLRPVADELRYHGWKVCVGASGTVQAL
                     QEIMMAQGMDERITLEKLQQLKQRAIHCGRLEELEIDGLTLERALVFPSGLAILIAIF
                     TELNIQCMTLAGGALREGLVYGMLHLTVEQDIRSRTLRNIQRRFMIDIDQAQRVAKVA
                     ANFYDQVENEWHLEAISRDLLISACQLHEIGLSVDFKQAPQHAAYLVRNLDLPGFTPA
                     QKKLLATLLLNQTNPVDLSSLHQQNAVPPRVAEQLCRLLRLAIIFASRRRDDLVPEMT
                     LQANHELLTLTLPQGWLTQHPLGKEIIDQESQWQSYVHWPLEVH"
     gene            complement(23480..25501)
                     /gene="rep"
                     /locus_tag="ACHKBL_000661"
     CDS             complement(23480..25501)
                     /gene="rep"
                     /locus_tag="ACHKBL_000661"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HFO5817579.1"
                     /translation="MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCG
                     YQARHIAAVTFTNKAAREMKERVGQTLGRKEARGLMISTFHTLGLDIIKREYAALGMK
                     ANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPAQAAAEAKGER
                     DRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQRNEEVRERWQNKIRYLLVDEYQD
                     TNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALKVIKLEQN
                     YRSSGRILKAANILIANNPHVFEKRLFSELGYGTELKVLSANNEEHEAERVTGELIAH
                     HFVNKTQYKDYAILYRGNHQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRV
                     LTNPDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTLSGRGY
                     EALTRFTHWLAEIQRLAEREPIAAVRDLIHGMDYESWLYETSPSPKAAEMRMKNVNQL
                     FSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESEEELDQVQLMTLHASKGLEFP
                     YVYMVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQKELTFTLCKERRQYGELVRP
                     EPSRFLLELPQDDLIWEQERKVVSAEERMQKGQSHLANLKAMMAAKRGK"
     gene            25588..25869
                     /gene="ppiC"
                     /locus_tag="ACHKBL_000662"
     CDS             25588..25869
                     /gene="ppiC"
                     /locus_tag="ACHKBL_000662"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PpiC"
                     /protein_id="HFO5817580.1"
                     /translation="MAKTAAALHILVKEEKLALDLLEQIKNGADFGKLAKKHSICPSG
                     KRGGDLGEFRQGQMVPAFDKVVFSCPVLEPTGPLHTQFGYHIIKVLYRN"
     gene            complement(25915..27390)
                     /gene="ilvC"
                     /locus_tag="ACHKBL_000663"
     CDS             complement(25915..27390)
                     /gene="ilvC"
                     /locus_tag="ACHKBL_000663"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004455 - ketol-acid reductoisomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HFO5817581.1"
                     /translation="MANYFNTLNLRQQLAQLGKCRFMGRDEFADGASYLQGKKVVIVG
                     CGAQGLNQGLNMRDSGLDISYALRKEAIAEKRASWRKATENGFKVGTYEELIPQADLV
                     VNLTPDKQHSDVVRTVQPLMKDGAALGYSHGFNIVEVGEQIRKDITVVMVAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGMAIAKAWAAATGGHRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSLLCFDKLVEEGTDPAYAEKLIQFGWETVTEALKQGGITLMMDR
                     LSNPAKLRAYALSEQLKEIMAPLFQKHMDDIISGEFSSGMMADWANDDKKLLTWREET
                     GKTAFETAPQYEGKIGEQEYFDKGVLMIAMVKAGVELAFETMVDSGIIEESAYYESLH
                     ELPLIANTIARKRLYEMNVVISDTAEYGNYLFSYACVPLLKPFMAELQPGDLGKAIPE
                     GAVDNAQLRDVNEAIRSHAIEQVGKKLRGYMTDMKRIAVAG"
     gene            27540..28430
                     /gene="ilvY"
                     /locus_tag="ACHKBL_000664"
     CDS             27540..28430
                     /gene="ilvY"
                     /locus_tag="ACHKBL_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001053418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HFO5817582.1"
                     /translation="MDLRDLKTFLHLAESRHFGRSARAMHVSPSTLSRQIQRLEEDLG
                     QPLFVRDNRTVTLTEAGEELRVFAQQTLLQYQQLRHTIDQQGPSLSGELHIFCSVTAA
                     YSHLPPILDRFRAEHPSVEIKLSTGDAADAMEKVVTGEADLAIAGKPETLPGAVAFSM
                     LENLAVVLIAPALPCPVRNQVSVEKPDWSTVPFIMADQGPVRRRIELWFRRNKISNPM
                     IYATVGGHEAMVSMVALGCGVALLPEVVLDNSPEPVRNRVMILERSDEKTPFELGVCV
                     QKKRLHEPLIEAFWKILPNH"
     gene            complement(28427..29971)
                     /gene="ilvA"
                     /locus_tag="ACHKBL_000665"
     CDS             complement(28427..29971)
                     /gene="ilvA"
                     /locus_tag="ACHKBL_000665"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HFO5817583.1"
                     /translation="MADSQPLSGAPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRL
                     DNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLG
                     VKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHP
                     MVIAGQGTLALELLQQDAHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSA
                     CLKAALDAGHPVDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKD
                     LFEDVRAVAEPSGALALAGMKKYIAQHNIRGERLAHILSGANVNFHGLRYVSERCELG
                     EQREALLAVTIPEEKGSFLKFCQLLGGRSVTEFNYRFADAKNACIFVGVRLSRGLEER
                     KEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESPGALLRF
                     LNTLGTHWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGYDCHDETNNPAF
                     RFFLAG"
     gene            complement(29974..31824)
                     /gene="ilvD"
                     /locus_tag="ACHKBL_000666"
     CDS             complement(29974..31824)
                     /gene="ilvD"
                     /locus_tag="ACHKBL_000666"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HFO5817584.1"
                     /translation="MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFT
                     QFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSDQII
                     KLDLVDAMIQGADPKVSDSQSDQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGN
                     GSLLATHADRKQLFLNAGKRIVELTKRYYEQNDDSALPRNIASKAAFENAMTLDIAMG
                     GSTNTVLHLLAAAQEAEIDFTMSDIDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIG
                     ILGELDRAGLLNRDVKNVLGLTLPQTLEQYDVMLTQDDAVKNMFRAGPAGIRTTQAFS
                     QDCRWDTLDDDRANGCIRSLEHAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTG
                     PAKVYESQDDAVEAILGGKVVAGDVVVIRYEGPKGGPGMQEMLYPTSFLKSMGLGKAC
                     ALITDGRFSGGTSGLSIGHVSPEAASGGSIGLIEDGDLIAIDIPNRGIQLQVSDAELA
                     ARREAQEARGDKAWTPKNRERQVSFALRAYASLATSADKGAVRDKSKLGG"
     gene            complement(31889..32818)
                     /gene="ilvE"
                     /locus_tag="ACHKBL_000667"
     CDS             complement(31889..32818)
                     /gene="ilvE"
                     /locus_tag="ACHKBL_000667"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HFO5817585.1"
                     /translation="MTTKKADYIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYD
                     SHKGPVVFRHREHMQRLHDSAKIYRFPVSQSIDELMEACRDVIRKNNLTSAYIRPLIF
                     VGDVGMGVNPPAGYSTDVIIAAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAK
                     AGGNYLSSLLVGSEARRHGYQEGIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSAL
                     PGITRDAIIKLAKELGIEVREQVLSRESLYLADEVFMSGTAAEITPVRSVDGIQVGEG
                     RCGPVTKRIQQAFFGLFTGETEDKWGWLDQVNQ"
     gene            complement(32838..33101)
                     /gene="ilvM"
                     /locus_tag="ACHKBL_000668"
     CDS             complement(32838..33101)
                     /gene="ilvM"
                     /locus_tag="ACHKBL_000668"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HFO5817586.1"
                     /translation="MMQHQVNVSARFNPETLERVLRVVRHRGFHVCSMNMAAASDAQN
                     INIELTVASPRSVDLLFSQLNKLVDVAHVAICQSTTTSQQIRA"
     gene            complement(33098..34744)
                     /gene="ilvG"
                     /locus_tag="ACHKBL_000669"
     CDS             complement(33098..34744)
                     /gene="ilvG"
                     /locus_tag="ACHKBL_000669"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HFO5817587.1"
                     /translation="MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLC
                     RHEQGAAMAAIGYARATGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAP
                     FIGTDAFQEVDILGLSLACTKHSFLVQSLEELPRIMAEAFDVASSGRPGPVLVDIPKD
                     IQLASGDLEPWFTTVENEVTFPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVSALRE
                     FLAATKMPATCTLKGLGAVEADYPYYLGMLGMHGTKAANFAVQECDLLIAVGARFDDR
                     VTGKLNTFAPHASVIHMDIDPAEMNKLRQAHVALQGDLNALLPALQQPLNIDDWQQHC
                     AQLRDEHAWRYDHPGDAIYAPLLLKQLSDRKSADCIVTTDVGQHQMWAAQHIVHTRPE
                     NFITSSGLGTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKI
                     VLLDNQRLGMVRQWQQLFFQERYSETTLTDNPDFLMLASAFGIPGQHITHKDQVEAAL
                     DTMLNSDGPYLLHVSIDELENVWPLVPPGASNSEMLEKLS"
     gene            complement(34747..34797)
                     /gene="ilvX"
                     /locus_tag="ACHKBL_000670"
     CDS             complement(34747..34797)
                     /gene="ilvX"
                     /locus_tag="ACHKBL_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide IlvX"
                     /protein_id="HFO5817588.1"
                     /translation="MNNSTKFCFSRFRTGN"
     gene            complement(34884..34982)
                     /gene="ilvL"
                     /locus_tag="ACHKBL_000671"
     CDS             complement(34884..34982)
                     /gene="ilvL"
                     /locus_tag="ACHKBL_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ilv operon leader peptide"
                     /protein_id="HFO5817589.1"
                     /translation="MTALLRVISLVVISVVVIIIPPCGAALGRGKA"
     gene            35335..36855
                     /gene="yifB"
                     /locus_tag="ACHKBL_000672"
     CDS             35335..36855
                     /gene="yifB"
                     /locus_tag="ACHKBL_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HFO5817590.1"
                     /translation="MSLSIVHTRAALGVNAPPITVEVHISKGLPGLTMVGLPETTVKE
                     ARDRVRSAIINSGYEYPAKKITINLAPADLPKEGGRYDLPIAIALLAASEQLTANKLD
                     EYELVGELALTGALRGVPGAISSATEAIKSGRKIIVAKDNEDEVGLINGEGCLIADHL
                     QAVCAFLEGKHALERPKPTDAVSRALQHDLSDVVGQEQGKRGLEITAAGGHNLLLIGP
                     PGTGKTMLASRINGLLPDLSNEEALESAAILSLVNAESVQKQWRQRPFRSPHHSASLT
                     AMVGGGAIPGPGEISLAHNGVLFLDELPEFERRTLDALREPIESGQIHLSRTRAKITY
                     PARFQLVAAMNPSPTGHYQGNHNRCTPEQTLRYLNRLSGPFLDRFDLSLEIPLPPPGI
                     LSKTVVQGENSTTVKQRVMAARERQFKRQNKLNAWLDNPEIRQFCKLESEDAQWLEET
                     LIHLGLSIRAWQRLLKVARTIADIDQSDIITRQHLQEAVSYRAIDRLLIHLQKLLT"
     gene            complement(36881..37219)
                     /gene="maoP"
                     /locus_tag="ACHKBL_000673"
     CDS             complement(36881..37219)
                     /gene="maoP"
                     /locus_tag="ACHKBL_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ori organization protein MaoP"
                     /protein_id="HFO5817591.1"
                     /translation="MAESFTTTNRYFDNKHYPRGFSRHGDFTIKEAQLLERHGYAFNE
                     LDLGKREPVTEEEKLFVAVCRGEREPVTEAERVWSKYMTRIKRPKRFHTLSGGKPQVE
                     GAEDYTDSDD"
     gene            37338..38177
                     /gene="hdfR"
                     /locus_tag="ACHKBL_000674"
     CDS             37338..38177
                     /gene="hdfR"
                     /locus_tag="ACHKBL_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator HdfR"
                     /protein_id="HFO5817592.1"
                     /translation="MDTELLKTFLEVSRTRHFGRAAESLYLTQSAVSFRIRQLENQLG
                     VNLFTRHRNNIRLTAAGEKLLPYAETLMSTWQAARKEVAHTSRHNEFSIGASASLWEC
                     MLNQWLGRLYQNQDVHTGLQFEARIAQRQSLVKQLHERQLDLLITTEAPKMDEFSSQL
                     LGYFTLALYTSAPSKLKGDLNYLRLEWGPDFQQHEAGLIGADEVPILTTSSAELAQQQ
                     IATLNGCTWLPVSWARKKGGLHTVVDSTTLSRPLYAIWLQNSDKNALIRDLLKINVLD
                     EVY"
     gene            complement(38272..38347)
                     /locus_tag="ACHKBL_000675"
     tRNA            complement(38272..38347)
                     /locus_tag="ACHKBL_000675"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(38312..38314),aa:Trp,seq:cca)
     gene            complement(38356..38432)
                     /locus_tag="ACHKBL_000676"
     tRNA            complement(38356..38432)
                     /locus_tag="ACHKBL_000676"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(38396..38398),aa:Asp,seq:gtc)
     gene            complement(38487..38602)
                     /gene="rrf"
                     /locus_tag="ACHKBL_000677"
     rRNA            complement(38487..38602)
                     /gene="rrf"
                     /locus_tag="ACHKBL_000677"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         9461 a        10556 c         9756 g         8890 t
ORIGIN      
        1 gccagaggta ggattgaaaa cgctctcctg attttccaat tcattttcta gataaataaa
       61 taatttattt ttatcactat tatttatgca atcatcctgt cagggagagg gatctcaatt
      121 atcaatgctt aattacgtca tcattttgac gacatgaaac cggagcattt tgcatggaat
      181 tttcgttttc acccaaactt cttgttgtgg ctgtcgccgc cgctcttcca ctcatggcca
      241 acgcagcaga taccccgtca accgccaccg cacgcaaagg ctttgccgga tacgatcacc
      301 caaaccagta tctggttaaa ccggcgacca ctattgccga caacatgatg ccggtgatgc
      361 agcatcctgc acaagacaaa gaaacccagc agaagctggc agaacttgag aaaaaaaccg
      421 gtaagaaacc gaacgtggtt gttttcttgc tggatgatgt gggctggatg gatgttggct
      481 ttaacggcgg cggcgtggcg gtgggtaacc ctacaccaga tatcgacgcc gttgccagcc
      541 aggggctgat tttaacttcg gcgtattctc aaccgagttc ttccccaacc cgcgccacga
      601 ttcttaccgg acaatactcc atccaccacg gcattctgat gccgccaatg tacgggcaac
      661 cgggcggtct gcaagggtta accacgctgc cgcagttgct gcacgatcag ggctacgtca
      721 ctcaggccat cgggaaatgg catatggggg aaaacaaaga gtcgcagccg cagaacgttg
      781 gctttgatga tttccgcggc tttaactcgg tatccgatat gtataccgaa tggcgtgatg
      841 ttcacgtcaa cccggaagtc gccctgagcc cggatcgttc tgaatacatc caaaaactgc
      901 cgttcagcaa agatgacgtt catgccgtgc gcggcggtga acaggaagcc attgccgaca
      961 ttacgccgaa atatatggaa gacctggatc aacgctggat ggaatatggc gttaagttcc
     1021 tcgacaagat ggcgaagagc gataagcctt tcttcctcta ctacggcact cgcggctgtc
     1081 acttcgataa ctacccaaat gcgaaatatg cgggtagctc tccggcgcgc acctcttacg
     1141 gcgactgcat ggtggagatg aacgatatct tcgccaatct gtataaagca ctggagaaaa
     1201 acggtcagct tgataacacg ctgattgtgt tcacctctga taacggtccg gaagccgaag
     1261 ttccgccgca cggtcgcaca ccgttccgcg gtgcgaaagg ttctacatgg gaaggcggcg
     1321 ttcgtgtgcc aaccttcgtt tactggaaag gcatgatcca accgcgtaaa tctgacggca
     1381 ttgtcgatct ggcggatctc ttccctacgg cgctggatct ggcggggcat cctggggcga
     1441 aagtagcgaa tttagtaccg aaaaccacct ttatcgatgg tgtggaccag acatctttct
     1501 tcctgggaac aaatggtcag tctaaccgta aggccgagca ctacttcctc aacggtaaac
     1561 tttctgctgt gcgtatggat gagttcaagt atcacgtcct gattcagcaa ccttacgctt
     1621 atacccagag cggatatcag ggtggattca ccggcacagt aatgcaaacg gcgggatcgt
     1681 cggtgtttaa cctgtacacc gatccgcagg aaagcgactc tatcggcgtg cgccatattc
     1741 cgatgggtgt accgctacag accgaaatgc acgcgtatat ggagatcctg aaaaaatatc
     1801 caccacgcgc gcagattaaa tctgactaag ccggcgctat cgctgagatc tgcctttgcc
     1861 ggatgcgatg ctgacgcatc ttatccagcc tacagaacgc tgcaatttat tgaatttgca
     1921 atgattgtag gccggataag gcgttcacgc cgcatccggc aatcaaccgc aggcggccgc
     1981 cgatttctac ttattcacca ccatcaaatg cgcctgcata atgtcgctgg ccggacgacc
     2041 gtgcgccagc aaatcagcca ttgctttaag atatggcggt agatggcgga aataacgctg
     2101 atacccggca cacaaataat tcagcccggg tttgccgctg gcatcgagca taaagcggtg
     2161 tttcgggcag cctccccagc acgcttttaa cacgttgcaa ctgcgacact gtgccggtaa
     2221 ctgcttgaat ttatcttcac caaatgcctg ctgttgcggg gaatcaatca tttctgcaat
     2281 cgtctgctga agcatattcc ctagtcgata ctgcggataa acatagtgat cgcaggcgta
     2341 aacatcgccg ttgtgttcaa caatcaccga gcgcccacag gttggctgat gatggcaaac
     2401 cgcgcccggt gcgccgacaa aattggcgaa cgcccattcg atattcatta cgaaaacctt
     2461 gccgacgtcg cgcttaatcc agtggtcgaa aatcgccacc agaaactcac cgaactcttg
     2521 cgggcacacc gaccattccg tcagttcgcc ctgaatatcg ccgggagcat gtagcttaag
     2581 tccagcatga gcagccgttt catcagccag gcgctcgacc accggaataa actggatgaa
     2641 ctcaacacct gcatcacaca gaaaatcata gacttgcaaa ggttgtaacg cactggtgcg
     2701 atttacgcag accagcacgt tatagtcgac atgatgtttt tgcaggagcg tcagggcacg
     2761 catcaccagc ttatgcgttg gtctgccgcc tttggtcacg cgatattgat tgtggatctc
     2821 agccggacca tccagcgata acccaacaag aaaatgattt tctgccagaa atgcacacca
     2881 ttcgtcatca agcagcacgc cgttagtctg gaagctgtta cttatcttcc tgccagcacc
     2941 atatttcgcc tgtagcgcca cggcacggcg gtaaaaatcc agcccgagta acgttggttc
     3001 gccgccctgc caggtaaaag ccacttcgtt ttgggtttcg ctggcagcga tatagtgacg
     3061 gacatacgct tccagcgtgt catcgtccat atgcgtgact ggcttttcgc ggtaaaggga
     3121 ttgtttttcg agataaaaac agtagtcaca gttcagatta caatcggaac cgctcggttt
     3181 cgccatcaca tgaaaagcac gcgttggaac ctgttgcagc atggtcgctc cttgcctgaa
     3241 gtcatccttc aataagttaa gggcgaaatc ataccgtggc gcaatgcgtt attacgggcg
     3301 tttattgcga agggaaagtg acgaaatgca cagaaaacaa aaaaccccgc cgaagcgggg
     3361 ttcaaaattg gtcggcgaga gaggattcga acctccgacc cactggtccc aaaccagttg
     3421 cgctaccaag ctgcgctact cgccgaaata ctgctttttg aatttttagt tcaattcttt
     3481 aaagtcgtgg tgcgatgggg gggacttgaa cccccacgtc cgtaaggaca ctaacacctg
     3541 aagctagcgc gtctaccaat tccgccacct tcgcattgtc acaacttcta ataatggggt
     3601 ggctaatggg attcgaaccc acgacaactg gaatcacaat ccagggctct accaactgag
     3661 ctatagccac cactacaaat cttgttacgc ggtattacta ccaccgcagc tcaagcgccg
     3721 ggactaaatg gcgcgcccga caggattcga acctgagacc tctgcctccg gagggcagcg
     3781 ctctatccag ctgagctacg ggcgcttagc gccgttgcgg gggtggataa tacggacttc
     3841 ccacccctct gtctagtccc tttttaaata aattgcgcgt ttggttacgg tttgtgcgct
     3901 ttgctgctta tttctccagt ttatgcgctt taccgtggcg attaagccca aaaactttat
     3961 aaatcgccgt caccgtcagc ataaagatga tgccgacaaa cagcgacata cgcgtatctt
     4021 cattaaagta catgccgatc aaaacgcaaa tcaggaatgc cattgttacg tagttggccc
     4081 acgggaacag gattgagcgg aacggatggc tggcaattgc cgctttatgc gcacgacgaa
     4141 aacgcagctg gctaatcaat atcacaaacc atggcaccat ccccggaagc acgctggcac
     4201 tgtagacgta gacaaacaca cgctgcggat tgggaatgat gtagttcagg catgagccaa
     4261 ttaacaggat agcaatagat actgccacac ccgcaaccgg tacgccgtga cgggaaactt
     4321 tcgccatcgc cgtcggtaac tgacggtttt tcgccaatgc gtagagcata cgtccgcaac
     4381 tgtacatgcc gctgttacag ccagagagcg cagccgtcag caccacaaag ttgataatgc
     4441 ccgccgctgc ggtaatgccg attttggcga aagtcagtac gaacgggctg ccgttactgc
     4501 ctatttcatt ccacgggaag atggtgacga taacgaaaat cgcgcctacg tagaaaatca
     4561 ggatacgcca caacactttg cctacggcac tgcgcagcgt cacctgcgga ttcttcgctt
     4621 caccggcagt aatgccaatc agctccacgc cctggtagga cgccaccaca atacacaatg
     4681 cggtcaggaa ccctttccag ccacccgcaa agaaaccgcc atgctctgtg agattgctaa
     4741 aaccaatcga ctgcccgcca ttgccaaaac caaagaaaat cacgcccagg ccaatgacaa
     4801 tcatcacgat aatcgtggtg actttgatca tcgcgaacca gaactcgatt tcgccgtaca
     4861 accgcactgc cgccagattc gccagcgcca ccagcgccac cgcgatcaat gcgggtatcc
     4921 actgcgccat ctccgggaac cagaactgga cataaacgcc aatggcggtg atttctgaga
     4981 tccccaccgc catccacata aaccagtaag accaggcggt gagatagcca aaaaacgggc
     5041 tcatataacg atgcgcataa acggcgaacg aaccggtaac cggttcgagg aacaacattt
     5101 cgcccattga acgcatgatg aaaaagacga acagcccggc gatgatatag gccaacaata
     5161 cggatggccc ggcccatttc agggtactgg cggcccccat aaacaggccg acgccaatgg
     5221 tgccccccag ggcgatgagt tcgatatgtc gagcttccag cccacgctgt agctctggtt
     5281 tgttatctgc cataaatcct cgtgttgtgt ttgcatgctt tccggtgtta ccggttatcg
     5341 ttatgggtac atcgagtgtt gcgaatgttt tcgtaattca ggagaaatgg caaataaagc
     5401 attaaaaatt tgaatgcttt gtgtaataaa aaagcagaca ggcgacggag tgaccactcc
     5461 gtcgctttac aaagagagga aaatcagagg ttgccggtgt agtgccagcg taaataacgc
     5521 agcaaacgaa gctgacgctt aatgcggctc ggctgcgaaa gcaggcggta gagccactcc
     5581 agccccagcg tttgccagat tttcggtgcg cgttttacgt gaccggtgaa aacatcgtag
     5641 gtcccgccaa cgcccatata cagcgcatct ggatgcacca gacggcagtc gcgcatgaag
     5701 atctcctgct ttggcgatcc catcgcaacg gtgacgattt gcgctccgct ggcatgaatg
     5761 cgttcaaaca gcgcctgacg ctgctcgggt ttaaaataac catcctgact gccaacgata
     5821 ttcacattcc actggttgcg cagtttagct tcagtttgcg ccagcacttc aggtttaccg
     5881 cccacaagaa ataccggcgt cccttctttg cctgcgcgcg ccatcagctc ttcccagaga
     5941 tcggcaccgg caacgcggga aacctgcgcc tgcgggtact ttttacgtac tgaacgtaca
     6001 acgctgatgc catccgcata tttaaattcg gcagcgttaa ttagctccct gacctcagca
     6061 ttatcttcaa tggtcagcat tttttcggca ttaatggcaa ccagcgttcc ctgcttaagc
     6121 tgcccgtcag caaacagata atcgagggcg tgctgcatat cacgccaacc aatcaactgt
     6181 aagccacgca gcgtataggt cggtgccgtg gtgttgttat tcattgttat ccttcaacct
     6241 gcgtccggag cgatgatttt gtacgtttat gaatgagtcc ggcgctttca aaaagccagt
     6301 acaacagttt tgcgatcatc agacatgcgc cgaagaccac gataaaaaag actacgcgtg
     6361 agacaaacga atccagccct tcacgtgcca gcacgatcat attgaagatc gccccgaagc
     6421 agaaactgtg caatatcgca gccttatagc gattagtctc gcggttgccc agctcataca
     6481 gccagtcgaa ccatttgatg atcagaccaa ccacaatcgc cccgagcggg atgaacaacg
     6541 cgccgcccat caccaccagt gagcctataa gcgtaggcga gatcgccagc ccggagtggt
     6601 tattcagcac ttcccaggta aagtagttgg cggagttcag taccatactc gggcgacccg
     6661 gccacagcca ggaagggata aagacataga aatcgcggac aatgggagcc aggccctgga
     6721 agtcgatgtt gtcgtagttc tgcaacagca acgccagatt ctcccacggc gaaaaggtat
     6781 cgcgggtgag atagagaaac gtatagaacg cttcatcgcc gctcacattc attccataac
     6841 gttttagcgc cagccagaac atgcccacaa tccccagcac gcccgccgcc gccagcatcc
     6901 acaacgaaat ccagccgcga ataatgccaa taaacaggaa gatagcgaat gcgatgatga
     6961 tattggcgcg agtgccgccg acaatcatgt aagtcaacaa gccaaaggca accgtgctga
     7021 cgaggaaaaa cagccacgct ttgctatcct ggcgcagaaa ataaaccacc agcatcgccg
     7081 ggatgaaaaa gtaaaagaag cgttttaatg ccacgccgga gacttcgctg gaaaagatct
     7141 gactgtaaga attaagccgg aacagcaaaa agccgttgtg catgaagaag atgccgacgc
     7201 tcaccagagc gatgcccatc aggatcaccc acgtgagatt ggtctccacg cggttcatgg
     7261 taaacagcgg acggcgcggt gcatcagcaa cgcgtttgcg tagacgggtt ttgtaggtga
     7321 catagtaaac cgcgtagaag cagcctgcag aaagcaacgc ctgcaacaag atttctggcg
     7381 gcgcgacacc aacatcaaag cgaaatacca gcacgctggt cagcggaaag ccgaagaaaa
     7441 aggtgagcaa aaacagcaat gaaaagaaga cattgaagtt aaagcgcaca cggcgaaact
     7501 caaaccaggt cagcgtggca ataaacaacg tgcagagcag ccagacaaca aacaggccac
     7561 tgaattgcag cagactcatg cgacctcccc ggcggcaatc gccaacgccc gctgccagcc
     7621 ttgtagatag ttagggctaa agaaggcaat ggtgttttta tccaccgacg ccaactggcg
     7681 ctgcgcttca cgcacaatat cctcgttgag atcatcggta gtaaacagta ctggcaaatg
     7741 ctgttccgtc atatcctgcc agaacggatt ttcccggtta agcacacaag gaatgcccgc
     7801 ctgaatcagt aagcaaagcg taccaatgcc ctgctggcgg gcaaaaataa agtaaccgag
     7861 atcgcactga cgaagtagcg tcagataggc gtcaaattcc agtttttcgc tcagaacttg
     7921 cagattttct tcgctgaata actccagtcc cgcctgacga acttcctcga tgtacgcttc
     7981 gttattaggc ggatatccca tcggcaccac cacttttacc gtatcgccaa attgctgatg
     8041 aacggcgcgc aaggcagcaa tatgctcatt gctgcggtca ccggagttcc ccaccagaat
     8101 ggtcattttt acttcacgtt gccgatcgtt caccatcgta ttaagagaag agtccatccg
     8161 cgtcgggaag tacagcagtt cgccccgcac ctttgggtgc gttttggcaa aaaagctcaa
     8221 atcaccgcgg gtggcaaata cacagccgac tcgcttttgc gccaggcgac gtagtgggta
     8281 aaaaagctta tatctcaagc cactggaaag ttcgtacagg tctgcccccc agatatgcca
     8341 gtaaaactgg ctgggcttaa tgccaccact cagcagagcc agccacagtg tgggattgaa
     8401 ctgaccgtgg aagaaaaaac gctgctgacg gttagctttt gctttcgcga tgaccgcttc
     8461 cgccagcgat tttttcccag ggaaaaattg cacagaaagc gccagacagc tatcacttaa
     8521 gccgtcgtcc ttgccaacaa ccataaactc gcgcgcgtgc tcgctcgtcg cggccagcgc
     8581 gtcgttgaaa aaccgcagaa cggttcggtt atggtgaggg atatccgatc ccagtacgtg
     8641 aatcagtaca gtcatgcccg cctacgccag agtaaaaaca cgccacaaca aagagaaaaa
     8701 tagacgatat aggttgccat ataggcctgc gctgcgccca gtgcaccatg cgcagggatt
     8761 agccagtggg caaataccat caataaagtg aactggctga cttccgccag aatataaaac
     8821 cgcagtgacg ctttggcgat caccagataa ccaaagacat aagcgcccac ttttaacaca
     8881 tcacccacta actgccaggc aaagagatcg cgcatagcgg taaatttatt cgacaacagc
     8941 agccagatag caaaatcacg cagcaaccag acggtaaaac tcgccgccgc cactgccggt
     9001 aagacgaatt ttagcgattt aaccacttcc cgggtgatat cgcgcttttc cgttagccgc
     9061 gacaacgtgg gcagcaaata tacgctgaac gatgccgtaa taaattgcag gtaggcatcg
     9121 gaaatactgc tcaccccttg ccagatcccc acctcatccc agctatactg cgccgccagc
     9181 agtttacgca tcatgatgta agcaacaggc aaggtcaccg acgtaatcaa cgccatgagc
     9241 gtaaatttgc tcaactgccc tgccagaccg ttatcccagc tgggtttcag atagcttaac
     9301 gggatgacac cacgtttgat caacatgatg gcggcaggaa ttaccaccag cgcgggaatc
     9361 agcgccagac ccagcaacgc cccttcataa ccgcccaaac ggtaactgac gtagtacgca
     9421 agaacgccaa tcaagctgcc aacaatcagc gataacgcat tacctgcggc atcgcgaaag
     9481 cctttcatca acgccagtaa caggttgccc caggcgatcc ccatttgcac caacgccact
     9541 aaacgcacca gctcctgata gtcggtatta ccaaacaatc cctggctgat tggcgcagct
     9601 gccagcacaa aaaccagcgc catcagcgta gagaaaccaa gtaccatcgc tgatgaagtg
     9661 ccgaccacgc ggcgcagctg ttggggatta tcatggtact gggcaacgta tttggttacg
     9721 ccgttaaaga tgcctgcccc ggcaagcacg ccgagcacgg taatcaactg gcggaaattt
     9781 gccgccagcc caagccccgc cggaccaaat gacactgcca gcaacttacc gaccagtaac
     9841 ccggcaccaa tcttgaccag tgtactggcc gcagtccaca aggacgcttt tgccaacgac
     9901 atatcaggag aagtagttca acaaggtcgc aattaccgta cgctgattga cgggcgacag
     9961 gttgtagaac aacggcaggc gcagcaggcg ctcgctctct ttggtggtgt agcgatcttc
    10021 accgtggaac tcaccaaagc gttcccccgc agggcaaccg tgcagcggaa tgtaatggaa
    10081 caccgccatg atttccgctt ctttcagaaa gttaatcaac gcgctccggt catcaatatc
    10141 ccgcagttta atgtagaaca tatgcgcgtt ctgcacgcag ccatcgggaa tcgacggcag
    10201 ctcgatacgc ccggctttcg ccagaggtgc taacgcatcg tagtagtttt gccacagcgc
    10261 cagacgttgc tgattgatac gatccgctgc ttccagttgc gcccacaggt acgcagcttg
    10321 caaatcggac atcaaatagc tggagccgat atcgcgccag gtatatttat cgacctgacc
    10381 acggaagaac tggctgcggt tagtgccttt ttcacggatg atctcggctc gttcgatcaa
    10441 cgctttatcg ttaatcagcg tcgcaccgcc ttcaccgccc gccgtgtagt ttttggtttc
    10501 atggaagcta aagcagccaa tatgaccaat ggttcccagt gcacgccctt tgtaagtgga
    10561 cataacgccc tgagcggcat cttccaccac aaacagatta tactttttcg ccaacgccat
    10621 aatggtgtcc atttcgcagg ccacacccgc gtaatggacc ggcacgataa cgcgcgtttt
    10681 gtcggtgatc gccgcttcaa tcagcgtttc gtcgatgttc atagtgtccg ggcgaacatc
    10741 cacaaagacg attttcgcgc cacgcagcac aaaggcattg gcggtggaga caaaggtgta
    10801 gctcggcatg atcacttcat cgccaggctg gatatcgagc agcagcgccg ccatctccag
    10861 agaagcggtg caggacggcg tcaacagcac tttggcgctg ccaaaacgtt gttccagcca
    10921 ctgctggcag cgacgggtaa aaccgccatc gccacacagt ttgccgctac ccattgccga
    10981 ctgcatatag tcgagttcgg ttcccaccac cggcggtgcg ttaaatggaa tcatgtgatc
    11041 acctgtataa ccagtacgcg gtgctttcta cattcgcacc actttgtatg tatcgtttaa
    11101 gcgcggcggt gttgcccatc tgggtcgcca cccgcaaagt tgttttaccg cgagcatacg
    11161 cccagtttag cgctgtttgc atcagctcag cacctgcacc gcgtccagcc agcaggccaa
    11221 ttcgcgcatc tgtcgcattg agttcccgta aagagacata gccgcgaata tcgccggacg
    11281 ccgaacgtaa aatgaggcat tgatgatcaa aggtgccgcg cacggcattt tcaatccact
    11341 gtgcataaaa gcgaccgctg gcgtcaggcg cataccacgg cgcacgaaaa cggctttgcg
    11401 caaatgcggc gctggctaac tgacgtaatg cgggaatatc ggtctcttgt gccactacag
    11461 caccactatc actgacattg tttacgggta gcgccaaatc aacttcacct tctaccaggg
    11521 agaatcccag ctgttgcaag gcatccagtt catccgtatt tgatgccgca attttggcct
    11581 gcacccgtga ccacggcgct aaggcgtctg gcgtcaggag cggtgcttca ggactaatgc
    11641 gcacaatggc gctgttaaca ccaaagaagg cgttttccca ggttagtggc tcaatactgg
    11701 cgcggacggg cacgaagtaa ctccagcaga tattggccgt agccagtttt cgccagcgag
    11761 ctggcggcgc gcttcacgcc ttcgtcatca agccagccgt tacgccaggc gatctcttcc
    11821 aggcaggcaa tcttaaagcc ctggcgtttt tccaccgtct gcacaaaggt gctggcttca
    11881 atcaggctgt cgtgagtgcc agtgtccagc caggcaaatc cgcgcccgag cagttcaacg
    11941 gtcaggttgc ccgcctcgag gtacatctgg ttgatggagg taatctccag ttcaccacgc
    12001 tccgacggct tcacctgctt tgcgtactcc acgactttac tgtcgtagaa ataaagcccg
    12061 gtcaccgccc agtttgactt cggctgtttt ggcttttctt ccagcgagat agcgcggaaa
    12121 ttgtcgtcaa actccacgac gccaaagcgt tccggatcca tcacctgata gccaaatacc
    12181 gtcgcgcctt gcgtgcgcgc cgcaacatga cgcagcttcg gactgaagcc ctgtccgaag
    12241 aagatgttat cgcccagcac cagacaagaa ggttcaccgt tgaggaaggt ttcaccgatg
    12301 ataaaggcct gcgccagacc gtccggactg ggctgttcgg catattccag ctggataccg
    12361 aactcactac catcgcccag caggcgctgg aaataacatt tatcttccgg cgtagtgatg
    12421 atgagaattt cgcggatacc ggccagcatc agcaccgaca gcggatagta aatcattggc
    12481 ttatcgtaaa tcggcaacag ttgcttcgat acgccgcgcg taatcggatg caatcgggtg
    12541 ccggaaccgc ccgccaggat aatacctttc atgtttgcct ccgctgaaaa ttagcctttc
    12601 agacctaaac gctcgccctg atagctgccg tcctgcacct gcttccacca gctttcatta
    12661 gccagatacc actgaaccgt tttacgcatt ccactttcaa aggtttcctg cggcagccag
    12721 ccaagttcac gggcaatttt cgaagcatca atggcataac gcagatcatg ccccggacgg
    12781 tcagcgacaa aggtgatcag gtcacgatag tgcgccacgc cgtgcggctt gttcggagcc
    12841 agttcttcca gcagctcgca aatggtttcc acaacatcga gattcttacg ctcgttgtga
    12901 ccaccaatat tataggtttc accgactttc ccggtggtcg ccacgcaata cagcgcgcgg
    12961 gcgtgatctt ccacatacag ccagtcacgg atttgctgcc cgttgccata taccggcagc
    13021 ggtttacccg ccagcgcgtt gaggatcatc agcgggatca gtttttccgg aaagtggtaa
    13081 gggccgtagt tattcgagca gttggtgata agcgtcggca gaccataggt ccgcagccag
    13141 gcgcgcacca gatggtcgct gctggctttt gacacggaat aagggctgct cggcgcatac
    13201 ggcgtggttt cggtgaagaa atcatccgtc gagtgcaggt caccatatac ttcgtcggta
    13261 gagatatgat gaaaacggaa cgctgatttt ttatcttccg tcagcgcatt ccagtaagcc
    13321 cgcgccgctt caagcaatgt ataagtcccg acaatgttgg tttcaacaaa cgctgccggg
    13381 ccgtcaatag aacggtcaac atggctttcg gctgccagat gcatgacaca gtctggctga
    13441 tgctcagtga atacgcgcgc cagttctgcc cgatcgcaga tatcaacttt ctcaaaggca
    13501 aagcgctcgc tttgcgcgac cggtgccagc gacatcagat ttccggcgta ggtcagctta
    13561 tcgaccacta ccaccgcgtc gctcgtttcg ttgatgatat aacgcaccag cgccgagcca
    13621 ataaacccgg caccacctgt tatcagaatt tttctcatca gcgccagact cctttggcat
    13681 cgacgacata ctgctgatgg acattgtcgc cattgataac tttgaactga ctatgatcga
    13741 ccagcatcac cagcacgtct gccgttgcca gcgcctcgtc aagctgcgcc agtgtacaaa
    13801 gcccggtcag ttttttcggc aactggtgga tgttaggctc aacaaccaga gtttcgccgc
    13861 tatgccactg ggcgatcagt tcagcgattt ccatcgctgg gctttcgcgc aggtcatcaa
    13921 tattcggttt aaacgccaga ccaaagcagg cgattttcag ttcactggcg cgtttatcgg
    13981 tagcagcaag gcagtcggca accgccgctt tcacctgatc gataacccag aacggtttgt
    14041 gatcgttcac ttcgcgcgcg gtacggataa gccgcgcctg ctgcgggttc tgtgccacga
    14101 taaaccacgg atcaacagca atgcagtgac cgcccacgcc aggaccaggc tgaagaatat
    14161 taacgcgagg gtgacgattc gccaggcgaa tcagttccca gacgttaatt ccctgatcgg
    14221 cacaaatcag cgacagttcg ttagcaaaag cgatattcac atcgcggaag ctgttttcgg
    14281 tgagcttaca catttccgcc gtccgcgagt tagtgacgac acactcacct tcgaggaaaa
    14341 ttttgtacag ttcgctggcg cgggccgaac aaaccggcgt cataccacca atcacgcgat
    14401 cgtttttaat cagctcgacc ataacctggc ccggtaacac gcgttccggg cagtaagcaa
    14461 tgttgacgtc cgccaactcg cccacctgct gtgggaaagt gagatctgga cgcatctctg
    14521 ctaaccattc tgccatcttc tcggttgacc ccaccggcga ggtggattca aggatcacca
    14581 gcgcgccttt tttcagcact ggcgcaatgg agcgagcagc cgattcaacg taggtcatat
    14641 ctggctcatg atcgccttta aacggcgtgg gtacagcaat cagccaggca tccgcttcaa
    14701 ctggcgtcgt gctcgctcgt aaaaaaccgc cttctacggc agtttttact acgctcgcca
    14761 aatcaggttc gacgatatgg atttcgccac gattgatggt atcaaccgcg tgttggttga
    14821 tatcgacacc aatcacctgt ttttgccgtg aggcaaacgc agctgctgta ggcagcccga
    14881 tgtaacccag tccgataaca gaaatggtcg caaaactcat agtgatatcc gattattttt
    14941 taacgcttcc agaatgcgag agcatgcctg accatcacca tacgggttat gggcgcggct
    15001 catagcttga tattcgtttt cgtcttttaa aagacgcgtc acttcctcga caattcgctg
    15061 cttatccgtg cctaccagac gcaccgtacc cgccgtcacc gcttccggac gctcagtggt
    15121 atcgcgcatt accagcaccg gtttccccag cgaaggcgct tcttcctgaa tgccgcctga
    15181 gtcggtcaaa atcagccagg cgtggttcat cagccagaca aacggtaaat actcctgggg
    15241 atcgatcaga atgacatttt tcacgtgccc cagaatgcga ttgaccggct ctctgacgtt
    15301 cgggttgaga tgcactggat agacaatctg gatgtcctgg tgcgtggtgg cgatgtctgc
    15361 cagcgcatgg cagatttctt caaagccacg accgaagctc tcacgcctgt gaccggtcac
    15421 cagaatcatt tttttattgg ggtcgataaa cgggtaattt gccgccagtt ctgaacgcag
    15481 cgtgtcgctg ctcatcacct ggtcacgcac ccataacagt gcatcaatga ctgtattacc
    15541 agtaatgaag attcggctat ccgcaacgtt ttcacgcagc aagttttgcc gggaagtttc
    15601 ggttggagag aagtgataca tcgccagatg cccggtcaat gtacggttag cctcttccgg
    15661 ccacggcgaa tagagatcgc ccgtgcgcag accagcctca acgtgaccaa caggaatacg
    15721 ctgataaaac gccgccaggc tggttgccag cgtcgtcgta gtatcgccgt gaaccagcac
    15781 gacgtctggt ttgaactcgg caagaatagg ttttagccct tccagaatcc gacaggttat
    15841 ctctgtaagg ccctgtcctg gctgcattat gttgagatcg tagtcaggta caatggaaaa
    15901 gagtttcagc acctgatcga gcatctcccg atgctgcgca gtgacgcaaa ctttagcctc
    15961 aaaaaaagga tcttttgcca acgcatgcac caacggcgcc atcttgatgg cttccgggcg
    16021 cgtaccaaat acagtcagta ctttcacatc gattctcttc gaataagcgg cgagcgcctt
    16081 tgcgctcacc gcagcagtgt tgctatttcg agcaacggcg ggttaatgcg acaccagccc
    16141 cgatcagtcc cccgacaatg ccccacataa tcatcaggaa ggcacgacgt gggctatcgc
    16201 gttttaccgg ttcttccggc gtacgaaaat agcgataggt ctgaaaacgc ggatccaggg
    16261 ttggaccaac attcagggtg tttaacatgg cccgattctg atcatagtcg agatcaaagg
    16321 ccggaccgac ggcctgtaaa ttttccagtc gagcctggag cattggacgc ccaagcagga
    16381 acatttctga atcaggtaat tcctcggcag gcacatctgt cgcactgcgc gaaatattat
    16441 gctgctcagc aattttcagc gcctgttcaa tactgttcat ccggcgatcg tagatggctt
    16501 tcgccacctc ttcctgacgc ttcacctgag ctttcatctg gatggtgcgt gccgcccatg
    16561 cgcctttcag ctcatcattc agatggctgg ctgcacgctg gctggcaaaa gcaacatact
    16621 gacgtaacag gttattagcg tcaggcgcag tttcggcaat cagcttcacg ctgtcattga
    16681 ccgcgcgggt gaagtcaccg ggaataaaca cgatgttgtt gatcatttca tccagcaacg
    16741 ccgcatcggc tttgctgttg cccaccatcc gctgtttgta atagtcggtt tgcagccaga
    16801 actctctgcg ggtatcccac gaggccagtt gcataacaaa ctctttataa gcttcgtcca
    16861 tgaccgatgg ttggtcggca gaagccatgt ttgaacggac atccaggtta cgcaaaaatt
    16921 gctgctgtga gtaatatccc cccagcatat tcaccgttgg acgatcggta atcgctgtcg
    16981 agctccactc ctggcgagca aaaaaagtat acgccagcgc gattaacgca aacgccagcc
    17041 ccatgccaat aatccacagc ttcccagccc acaaggtacg aaacaaccca cgaatatcca
    17101 gttcattttc agcgtcttcg gccggtttcc caggcattgg ttgtgtcatc acatcctcat
    17161 ttatttggtt aaattggggc tgccaccacg atttctacgc agtctgcgtt ttacgcgctt
    17221 aataaagcga gcaactttcc aggcacgctt gatgcaatat ccatagagga agaatgctag
    17281 caaaaagagc accagcatga cccactccgg gacaaaatga gaatattctg ccagcacgcc
    17341 aatggaagcg agcagtgctg cggcaagggt aatcagcaca aacgcctggc gggaagtaaa
    17401 cccggcacgc atgatcaaat ggtgaatatg ctgacggtct ggagagaatg ggctcatgcc
    17461 tttacgcagg cgacggtaca taatcgccac catatccatt agcggaatgg cgattatcca
    17521 caaagcggta accgggctga tgggatgagt tttgccctgg gtcgtttcga gcaggatcca
    17581 gataacggta aaaccaatca gcgtactgcc cgcatccccc ataaagactt tgtagcggcg
    17641 acccaggata ccaaggttaa gcatgatgta tggcaggatg gcggcgatca tcgcaaagca
    17701 ccagattgcg agactggttt gcccgtcgaa ccacaaaatc ataccgattg ctgcaaacga
    17761 gacgcaggac aacccaccca gcaagccatc aatgccatca accatgttga acgcattaat
    17821 ggccgcccag acggcaaata gcgtcaggaa gtaaccaaac ggtccgagca ccatctccca
    17881 ggagccaaag atataaccta ggctactgag ataaagtttg ccgaacacca tcataacaat
    17941 gccaacagcg gcctgtatgg tggcacggat ttttacgctg atatcaaaac ggtcatccag
    18001 cgcgccaatg aaaacaagca caccagcaca agcgagatag agagatgcat gcggaatata
    18061 gtaatcgaca attccgaacg tgaagcaaat ccctgcgtaa accgaaatcc ccccaacgag
    18121 aggtatcaat ccctggtgac gtttgcggaa gttaggttta tccactaaac cgactttttt
    18181 tgccacctta cgggcaaaaa acagaaacag tgtcgtgaat aaaaaaatac tgatgagatc
    18241 agtactcact gtcagtaaat tcacaatgca tgctctcaga gaaaattatt agcagaagta
    18301 taaccacgaa gacctttatt cagaagggaa agtccgttcc gaacccacca gtcctgtgca
    18361 aaaattaacc atttgatatg caaattgcat aacacaccag caaaatccag gaattttcct
    18421 aacattgcca gcgcggcatt aagattacag atataaaagc aaaacgccac gtaaacacgt
    18481 ggcgtttttg gcataagaca aatttatgag cgtttcatca tttcgaagaa atcgtcattg
    18541 gtcttggtca ttgccagttt attaatgagg aattccattg catcgatttc gcccatcggg
    18601 tgaatgattt tgcgcaggat ccacattttc tgcagttctt cctgagtagt gagcagctct
    18661 tctttacggg taccggaacg gttgtagtcg attgccggga agacgcgttt ttcagcgatc
    18721 ttacgagaga ggtgcagttc catgttgcct gtacctttaa actcttcgta gataacttcg
    18781 tccatcttgg aaccggtatc gataagcgcg gtagcgatga tggtcaggct gccgccctct
    18841 tccacgttac gcgccgcacc gaagaagcgt ttcggacgat gcagggcgtt ggcatccaca
    18901 ccaccggtca gcactttacc tgacgccgga acaacggtgt tgtaagcgcg cgccagacga
    18961 gtgatggagt cgagcaggat gataacgtct ttcttgtgct caacaaggcg tttcgccttc
    19021 tcgatcacca tttccgcgac ctgaacgtgg cgagatgctg gttcgtcaaa ggtagaagca
    19081 acaacttcac cttttaccag acgctgcatc tcggttactt cttccggacg ttcgtcgatc
    19141 agcagaacca tcagcacaca atccgggtgg ttgtaagcaa tgctctgagc aatgttctgc
    19201 agcagcatgg ttttaccggc tttcggcggt gccacaatca gaccacgctg accacgaccg
    19261 ataggtgatg ccagatccag tacgcgagcg gttaaatctt cagtagaacc gttaccacgt
    19321 tccatacgca gacgagagtt tgcgtgcagc ggggttaagt tctcaaagag gattttgttg
    19381 cgggcgtttt caggtttgtc gaagttaact tcgttaactt tcagcagcgc aaaatagcgt
    19441 tcaccttctt tcggcgggcg aatcttacca gagatggtat caccagtgcg gaggttgaaa
    19501 cggcggattt ggctagggga aacgtagatg tcatcaggac cggcgaggta ggagctgtct
    19561 gcggaacgga ggaaaccaaa tccatcctgc aatatctcca gtacaccatc accaaagata
    19621 tcttcgccac tctttgcgtg ctgcttcagg atggcaaaaa taatgtcctg cttacgcata
    19681 cgagccaggt tttccagccc catattttcg ccgagagtga tcagctcaga aaccggcgta
    19741 ttctttaatt cggtaagatt catagtggtg tgagttctta aacttggggt aaatctcgaa
    19801 cttaatgttg tgaatggtat ggcaggatca tccatgcctg tttacggtca tcgactcatg
    19861 tcttttcgct gcctggtcac aggagagtac gcagaactga aacgacaaga cggattgagt
    19921 gacgagccag aaatctgttc acttcgcatt taagataaaa cgggaagcgt tggaaattac
    19981 aagattcaaa cttaataagg tatgtttaat acgaagtcaa cactaagtta gcatgactca
    20041 cgccgggcgt ccagttttta gcgacggggc acccgaacat gaaattccct tacgccaggt
    20101 tagcgtcgag gaactctttc aactgacctt tagacagtgc gcccactttg gttgccgcca
    20161 cttcaccgtt tttgaacagc agcaaagtcg ggataccacg gatgccatat ttcggcgcag
    20221 tgccagggtt ttgatcgatg ttcagttttg caacggtcag tttgccctga tattcgtcag
    20281 cgatttcatc cagaatcggg gcgatcattt tgcacggacc gcaccactct gcccagaaat
    20341 cgacgaggat cgccccgtcc gctttgagta catccgtgtc aaaactgtcg tcagtcaggt
    20401 gaataatttt atcgctcata tataactcca caggaataag cctggcgtgt tggtttcgtt
    20461 gttggtgtaa cattaaccaa ctaaaggttg actttatttc accggatacg ctttcgtaaa
    20521 gcaatagtaa gctgatattc taccacacta tgagcaaaac acatttaaca gaacagaagt
    20581 tttccgactt cgccctgcat ccgaaggttg tagaagccct tgaaaaaaaa gggtttcata
    20641 actgtacgcc cattcaggca ctggcccttc cgctgacgct ggcgggtcgt gacgtagccg
    20701 ggcaggcgca aaccggtacc gggaaaacga tggcgtttct tacgtcaacg tttcattatc
    20761 ttctctctca tcccgcgatt gccgatcgca aggtgaatca accgcgtgcc ttaattatgg
    20821 caccgacgcg tgaacttgcc gtgcagatcc acgccgacgc ggagccactt gcgcaagcta
    20881 ccggcctgaa gctgggtctg gcttacggtg gtgatggcta cgacaaacag ctgaaagtgc
    20941 tggaaagcgg cgttgacatt ctgattggca ccacggggcg tttaattgac tacgccaagc
    21001 agaaccacat taacctcggt gccattcagg tagtggtact ggacgaagcc gatcgtatgt
    21061 acgatctggg ctttattaaa gatatccgct ggctgttccg ccgtatgccg cctgcaaacc
    21121 agcgcctcaa catgctgttc tccgccacgc tttcgtaccg ggtacgtgaa ctggcgttcg
    21181 agcagatgaa caatgccgaa tatattgaag tggaaccgga acagaaaacg ggccaccgta
    21241 ttaaagaaga gcttttctac ccttctaacg aagaaaaaat gcgtttgctg caaacgctga
    21301 tcgaagaaga gtggccagac agagcgatta ttttcgccaa caccaaacac cgttgtgaag
    21361 agatctgggg ccacctggcg gcagatggtc atcgtgtcgg tttgttgaca ggcgatgtcg
    21421 cgcagaaaaa acgtctgcgt attcttgatg aatttacccg tggtgatttg gatattctgg
    21481 ttgccaccga tgttgccgcg cgtggcctgc atatcccggc tgtgacgcac gtctttaact
    21541 acgatttacc cgatgactgt gaagattacg ttcaccgtat tggtcgtaca ggtcgcgctg
    21601 gcgcaagtgg tcattccatc agcctggcat gtgaagagta tgcattgaat ttgcctgcta
    21661 ttgagaccta tattggtcac tcaattccgg taagcaaata caatccggac gcattgatga
    21721 ccgatctgcc aaaaccgctg cgcctcacgc gcccgcgtac aggcaatggt ccgcgtcgta
    21781 ctggtgctcc gcgtaatcgt cgtcgttcag gttaaataaa atgtcatact caaaatcatt
    21841 caaactgtat caagatggca agagaatgaa tcccgatgcg catgctgtag taagtgattt
    21901 gggtgaaaga acgcagccaa cacagagaca gattgaagga tgaagagtat gggttccacc
    21961 tcgtcgctgt atgcagccat tgatctcggt tcgaatagtt ttcatatgct ggttgtgcgc
    22021 gaggtggctg gaagcatcca gacgctgacg cgaattaaac gcaaagtgcg tctggctgct
    22081 ggcctgaata gcgaaaatgc cctgtctaat gaagcaatgg agcgcggttg gcaatgtctg
    22141 cgcctgtttg ctgaacgtct gcaagatatc cctccctcgc aaattcgcgt tgtcgctacg
    22201 gcgacattac gactagccgt caatgcgggt gattttatcg ccaaagcaca ggaaatcctt
    22261 ggctgcccgg tacaagtcat cagcggtgaa gaagaagccc gcctgattta tcagggggtg
    22321 gcccacacaa caggcggtgc cgatcagcgc ctggtggtgg atataggcgg tgccagtact
    22381 gaactggtaa ccggcacggg tgcacaaacc acctcgttgt tcagcctgtc gatgggctgc
    22441 gtcacctggc tggaacgcta ttttgccgat cgtaatctgg cgcaggaaaa ttttgatgct
    22501 gcagaaaaag cggcacgcga agtgttacgt ccggttgcag atgaattacg gtatcacggc
    22561 tggaaagtgt gcgttggcgc ttccggcacc gtgcaggcgt tacaggaaat catgatggca
    22621 caggggatgg atgaacgcat taccctggaa aagttgcagc aactgaaaca acgcgccatt
    22681 cattgcggtc ggctggaaga gctagagatt gacgggctga cgctggaacg tgcgttagtg
    22741 ttcccgagtg gtctggcgat cctgatagcc atttttaccg aactgaatat tcagtgtatg
    22801 accctggcgg gcggtgcgct gcgtgaaggc ctggtctacg gtatgttgca tcttaccgtc
    22861 gagcaggata ttcgcagccg tacgctgcgt aatattcagc gccgctttat gatcgatatt
    22921 gatcaggcac agcgcgtagc caaagttgcg gctaacttct acgatcaggt ggaaaatgaa
    22981 tggcatcttg aagcaataag ccgcgatttg ctcatcagcg cctgtcagct tcatgaaatc
    23041 ggcctgagcg ttgacttcaa acaagcgccg caacacgctg cttatctggt acgtaatctg
    23101 gatcttcccg gttttacccc cgcacagaaa aaactgctgg cgacgctact gctcaaccag
    23161 actaatccgg tcgatctctc atcgctgcat cagcaaaatg ccgtaccacc gcgcgtcgca
    23221 gaacaactct gccgtttact ccgcctggct atcatttttg ccagccgtcg ccgtgacgat
    23281 ctcgtgccag agatgacatt acaggctaac catgaactgt tgaccttgac gcttccgcaa
    23341 ggttggctaa cccaacatcc gctgggtaaa gagattattg atcaggaaag ccagtggcag
    23401 agctatgtcc actggccgct ggaagtgcat taatgagtaa gtgccggatg cgatgctgac
    23461 acatcttttc cggccttgat tatttccctc gttttgccgc catcatcgct ttcagattcg
    23521 ccagatggct ttgccctttc tgcatccgtt cttcggcgct gaccactttg cgctcctgtt
    23581 cccaaatcaa atcatcctgc ggcagctcca gcaggaagcg gctcggctcc gggcgcacca
    23641 gttctccgta ctgacggcgt tctttacaca gcgtaaaggt caattccttc tgggcgcggg
    23701 taatgccgac ataggccagc cgccgctcct catcgatatt atcttcatcg atgctgctct
    23761 ggtgcggcaa aaacccctct tccataccga ccatgtagac ataaggaaac tccagccctt
    23821 tcgacgcgtg gagagtcatc agttgcacct gatccagctc ttcttcactc tcaccacgct
    23881 ccatcatgtc gcgcaaagta aagcgcgtca ccacctgggt gagcgtcatc ggctcatcca
    23941 gttcgctgcc ttccagcatc tccgtcatcc agctaaacag ttggttgacg ttcttcatgc
    24001 gcatttcggc ggctttcggg ctgggcgatg tttcgtacag ccaggattca taatccatgc
    24061 cgtggatcag atcacgcacc gcggcaatcg gctcccgctc cgccagacgc tggatttctg
    24121 ccaaccagtg agtgaagcgg gtcaatgctt cataaccacg tccgctaagc gtctgactca
    24181 ggcccatatc aaagctggcg gtaaacatgc ttttattgcg cgtcatcgcc cattcaccca
    24241 actttttcag cgtcgccggg ccaatctctc gcttcggcgt gttaacgata cgcaggaacg
    24301 cactatcatc gtccgggtta gtcagcacgc gcagataagc cagcaagtcc ttgatttcag
    24361 gacgagagaa aaacgacgta ccaccagaaa ttttgtacgg gatgcggttt tgcatcagga
    24421 atttttcaaa cacccgcgac tgatggttac cgcgataaag aatggcgtaa tctttgtact
    24481 gcgttttatt gacgaagtga tgggcaatca gctcgccagt aacgcgctcg gcttcatgtt
    24541 cctcgttatt cgcgcttaaa acttttagct ctgtgccgta acccagttcg gagaacagac
    24601 gcttttcaaa gacgtgcggg ttattggcga tcaggatgtt cgccgctttc aaaatacgtc
    24661 cggaagagcg gtagttctgc tcaagcttaa tcaccttcag cgccggaaaa tcctgactca
    24721 gcagcaccag gttttgcgga cgtgcgccgc gccaggagta gatcgactgg tcatcgtcac
    24781 ccaccacggt aaagcgcgcg cggctgccca ccagcagttt caccagctca tactggctgg
    24841 tgttggtgtc ctgatattcg tccaccagca ggtagcgaat tttgttctgc cagcgctcgc
    24901 ggacttcttc attgcgttgc aacagcaacg ttggcaataa aatcagatca tcgaaatcga
    24961 gaacgttaca ggctttcagg tgtgcatcat acagcccata acaatgggca aaaatacggt
    25021 cccgctcgcc ctttgcctct gccgccgcct gcgccggtgt tttgagatcg ttcttccagt
    25081 tggagatggt cgaaattagc tgttgcagga gaactttgtc atcttcaatc agcccctcgg
    25141 tcaactcttt aagcaaagca agctgatcgg tatcgtcaaa cagcgaaaag ttagccttca
    25201 tccccaacgc cgcgtactca cgcttaatga tatccagccc cagcgtgtga aaggtggaga
    25261 tcatcagccc acgcgcctct ttacgcccca gcgtctgccc gacacgctct ttcatctcgc
    25321 gtgccgcctt attggtaaag gtcaccgccg cgatatgtcg cgcctgataa ccgcaaccgc
    25381 ggatcagatg ggcgatttta ttggtgataa cacgagtttt accggaaccc gcgcccgcca
    25441 gcaccaggca ggggccggta acgaattcga cagcttgttg ttggccgggg tttagacgca
    25501 taggaaaatc gctcaatgaa agtcgggagg ggaaaataac agcgtggtag tatagccagc
    25561 ctcatcctta ccactcaagg cacgatcatg gcaaaaacag cagcagcact gcatatcctt
    25621 gtaaaagaag agaaactggc tctggatctt ctcgagcaga tcaagaatgg tgccgatttc
    25681 ggcaagctgg cgaagaaaca ctccatttgc ccgtcaggca aacgcggcgg tgatttgggt
    25741 gaattccgcc agggtcaaat ggttccggcg ttcgataaag tggttttctc ttgtccggta
    25801 ctggagccaa ccggcccgct gcatacccag ttcggttacc acatcattaa ggtgctgtac
    25861 cgtaactaac agcaaggcct tctccgggag aaggccttga actctgaatc gctattaacc
    25921 cgcaacagca atacgtttca tatccgtcat atagccgcgc agtttcttac ctacctgctc
    25981 aatcgcatgg ctgcgaatcg cttcatttac atcacgcagc tgcgcgttat ctaccgcacc
    26041 ttccggaata gctttaccca ggtcgcccgg ttgcagctct gccataaacg gtttcagcag
    26101 cggcacacaa gcgtaagaga acagatagtt accgtactcg gcagtatcgg agataaccac
    26161 gttcatttcg tacagacgct tacgggcgat ggtgttggca atcagcggca attcgtgcag
    26221 tgattcatag taagcagatt cttcgatgat gccggaatca accatggttt caaacgccaa
    26281 ctcaacgcct gcttttacca tcgcgatcat cagtacgcct ttatcgaagt actcctgttc
    26341 gccgattttg ccttcatact gcggcgcggt ttcgaatgcg gttttgccag tctcttcacg
    26401 ccaggtcagc agtttcttat cgtcgttggc ccagtcagcc atcatgccgg aggagaattc
    26461 gccggagatg atgtcgtcca tatgtttctg gaacagcggc gccatgatct ctttcagttg
    26521 ctcagagagc gcgtaagcac gcagtttcgc cgggttagaa agacggtcca tcatcagggt
    26581 gatgccgccc tgtttcagcg cttcggtgac ggtttcccaa ccgaactgaa tcagtttttc
    26641 tgcgtatgcc ggatcggtgc cttcttccac cagcttgtcg aagcacagca gagaaccagc
    26701 ttgcaacata ccgcacagga tggtttgctc gcccatcagg tcagatttca cttccgcaac
    26761 gaaagaggat tccagcacgc ccgcacggtg accgccggtt gcagccgccc atgctttagc
    26821 gatcgccatg ccttcgcctt tcggatcgtt ttccgggtga acggcaatca gcgtcggtac
    26881 gccgaatcca cgtttgtact cttcacgtac ttcggtgcca gggcatttcg gcgcaaccat
    26941 tacgacggtg atgtctttac ggatctgctc gcccacttcg acgatgttga aaccgtgtga
    27001 gtaacccagc gccgcgccgt ctttcatcag tggctgtacg gtgcgtacta catcagagtg
    27061 ctgcttgtcc ggcgtcaggt taaccaccag atccgcctgc gggatcagtt cttcgtaagt
    27121 acccacttta aaaccatttt cggttgcttt acgccaggat gcgcgcttct cggcaatcgc
    27181 ttctttacgc agagcgtaag aaatatccag accagaatca cgcatgttca ggccctggtt
    27241 cagcccctgt gcgccacagc cgacgatgac tactttttta ccctgaaggt agctcgcgcc
    27301 atcggcgaat tcatcgcgcc ccataaagcg acatttgccc agctgtgcca gctgctggcg
    27361 cagattcagt gtattgaagt agttagccat ggtgattcct cgtgatgttg tgcttcttat
    27421 tgttcggttc gcgtttgcga gattgaattc actatatgac aggaaattta ttgcggaaat
    27481 tgatatattc acaacgtcac attgcaattt ctgcaacgtc aacattgagg gctgcgccat
    27541 tggatttacg cgatctgaaa accttcctgc atctggcgga aagccgccat tttggccgta
    27601 gcgcgcgggc aatgcacgtt agcccgtcta cgctttcgcg acaaatacag cgcctggaag
    27661 aagatctcgg tcagccgctg tttgtgcgtg ataaccgcac ggtaacgctg acggaagcgg
    27721 gcgaagagct gcgcgttttc gcccagcaaa cgctgttgca gtatcagcaa ttgcgccaca
    27781 ccatcgacca gcaagggccg tcgctctctg gcgaattaca tatcttctgc tcggtgaccg
    27841 ctgcctacag ccatctgccg ccgattctgg atcgcttccg cgcggaacat ccgtcagtgg
    27901 agatcaaact ctctaccggt gatgcggccg atgcgatgga aaaggtggtc actggcgaag
    27961 cggatctggc gattgccggg aaaccggaaa ccttgccggg cgcagtggcg ttttcgatgc
    28021 tggagaatct ggcggtggtg ctgattgctc ccgcgctgcc ctgcccggtg cgtaatcagg
    28081 tatctgtcga aaagcccgac tggtctacag tgccgtttat catggccgat cagggaccgg
    28141 tacgtcgccg cattgaactg tggtttcggc gtaacaaaat cagcaaccca atgatttacg
    28201 ccacggttgg cgggcatgaa gcgatggtat cgatggtggc gctcggctgt ggcgtggcgc
    28261 tgttgccgga agtggtgctg gataacagcc cggaaccggt gcgtaaccgc gtgatgattt
    28321 tagagcgcag tgatgaaaaa acgccgtttg agctgggcgt gtgcgtacaa aaaaagcggc
    28381 ttcatgagcc gctgattgag gcgttctgga agattttgcc gaaccactaa cccgccaaaa
    28441 agaacctgaa cgccgggtta ttggtttcgt cgtggcaatc gtagcccagt tcattcagcc
    28501 gggtttcaaa atccggttca tgatcgccaa gctcgaacgc cgccagtacg cgcccgtagt
    28561 cggtaccgtg gctgcgataa tggaacagcg agatgttcca gtgcgtaccc agcgtgttga
    28621 ggaagcgcag cagcgcgccc ggcgattccg ggaattcgaa gctgtagagg cgttcctgca
    28681 acggatgcga tggacgcccg ccaaccatat agcgcacatg cagcttcgcc atttcgtcgt
    28741 cggagagatc caccacgctg tagccgccat cgttgagcat ctgcaaaatt tctttgcgct
    28801 cttcgaggcc acggctcagg cgcacgccga caaaaatgca ggcgtttttg gcatcagcaa
    28861 agcggtagtt gaactcggtg accgagcgcc caccaagtaa ctggcagaat ttgaggaagc
    28921 tgcccttttc ttccggaatg gtcaccgcca gtaacgcttc acgctgttcg cccagttcgc
    28981 agcgttccga gacgtagcgc agaccgtgaa agttcacgtt ggcaccggaa agaatatgcg
    29041 ccagccgctc accgcgaatg ttgtgctggg cgatgtattt tttcatccct gccagcgcca
    29101 gcgcgccaga gggttcagcc accgcgcgta catcttcgaa caggtctttc atcgccgcac
    29161 agatggcatc gctatcgacg gtgatgatgt cgtcaagata ctcctggcac aaacggaagg
    29221 tttcatcgcc gatgcgtttt accgcgacgc cttcagcaaa cagccccacg cggggcagat
    29281 caacgggatg acctgcatca agtgccgctt tcaggcaggc ggaatcttcc gcttctacgg
    29341 cgatcacttt gatttgtggc atcagttgtt tgatcagcac cgccacgccc gccgccagac
    29401 cgccgccgcc gaccggaaca aatacgcggt cgagatgagc atcctgctgg agcagttcca
    29461 gcgccagcgt accctgacca gcgatcacca tcggatgatc gaagggtggc acccaggtga
    29521 agccctgctg ctgtgacagt tcgattgctt tcgctttcgc ttcgtcaaag ttcgcgccat
    29581 gaagcagcac ttcgccgcca aagccgcgca ccgcatcaac tttgatatcg gcggtggcgg
    29641 ttggcatgac gatcagcgcc ttcacgccta accgtgcgga agaaaacgcg acgccctgcg
    29701 cgtggttacc cgcagaagcg gtaatcacgc cgtgcgcttt ttgttcttcc gtcaggcccg
    29761 ccatcatggc gtatgcacca cgaagcttaa agctgtgcac tggctggcga tcttcgcgct
    29821 tcaccagaat cacgttatca agacgcgacg acagtttttc cattttctgt agcggcgtga
    29881 cctgcgccgc ttcgtagacc ggtgcgcgca gcaccgctct taaatattcg gcaccttccg
    29941 gggcaccgga caggggttgt gagtctgcca tcattaaccc cccagtttcg atttatcgcg
    30001 caccgcgcct ttgtcggcgc tggttgccag gctggcgtag gcgcgcaggg caaaggagac
    30061 ctgacgttca cggtttttcg gcgtccaggc tttgtcaccc cgagcttcct gcgcttcacg
    30121 acgcgccgcc agttcggcat cgcttacctg taactgaata ccacggttcg gaatgtcgat
    30181 ggcgatcagg tcgccgtctt caatcaggcc aatgctgccg ccgcttgccg cttccggtga
    30241 gacgtggccg atagaaaggc cagaggtacc gccagagaaa cgaccgtcgg tgatcagcgc
    30301 acaggcttta ccgagaccca ttgatttcag gaagctggtt gggtagagca tttcctgcat
    30361 ccccggaccg cctttcgggc cttcatagcg aattactacc acatctccgg cgacaacttt
    30421 accgccgaga atcgcttcta ccgcatcgtc ctggctttcg tacactttcg ccgggccggt
    30481 gaatttgagg atgctgtcat ccacgcccgc ggttttaacg atgcagccgt tttccgcaaa
    30541 gttaccgtag agcaccgcca ggccgccgtc tttgctgtag gcgtgttcca gcgagcggat
    30601 acagccattg gcgcgatcgt cgtccagcgt atcccaacgg caatcttgcg agaatgcctg
    30661 ggtggtacgg atacccgcag ggccggcgcg gaacatattt tttaccgcgt catcctgggt
    30721 cagcataacg tcgtattgtt ccagcgtttg cggcaacgtc aggccgagca cgtttttcac
    30781 atcacggttc agtaaccccg cgcgatccag ttcgccgaga ataccgataa caccaccagc
    30841 acggtgaaca tcttccatat ggtatttctg ggtgctcggc gcaactttac acagctgtgg
    30901 aaccttgcgg gaaagcttat cgatatcact catggtgaag tcgatttccg cttcctgcgc
    30961 cgccgccagc agatgaagta cggtgttggt agagccgccc atcgcgatat ccagcgtcat
    31021 ggcgttttca aacgccgcct tactggcgat attacgcggc agtgcactat cgtcgttttg
    31081 ctcgtaataa cgtttggtca attcaacaat gcgtttacca gcattgagga acagctgctt
    31141 acggtccgcg tgggttgcca gcaacgagcc gttgcccggc tgcgacaggc ccagtgcttc
    31201 ggtcaggcag ttcattgagt tagcagtaaa catcccggag caggaaccgc aggttgggca
    31261 ggcggatcgt tcaacctgat cgctctggga gtcagaaact ttcgggtctg caccctggat
    31321 catcgcatca accagatcga gcttaatgat ctgatcggac agtttggttt tcccggcttc
    31381 catcgggccg ccggaaacaa agatcaccgg aatattcagg cgcagggaag ccatcagcat
    31441 ccccggggtg attttgtcgc agttagagat gcagaccatg gcgtcggcgc agtgagcgtt
    31501 gaccatatac tcaacggaat cagcgatcaa ttcgcgagat ggcagtgaat aaagcatccc
    31561 cccgtggccc atggcaatcc catcatccac cgcaatggtg ttgaactctt tggcaacgcc
    31621 gccagccgct tcaatttgtt cggcgaccag tttaccgaga tcgcgcagat ggacgtgacc
    31681 cggtacaaat tgggtgaacg agttcacaac cgcgataatc ggcttaccga aatcggcgtc
    31741 ggtcattccg gtggcgcgcc acagcgcacg agcacccgcc atattacgac catgagtggt
    31801 ggtggcggaa cggtacttag gcatacttta tttactccca gtgtctgtct cgtaaatggg
    31861 acggtgcgtg ccgtcccatt ttttgtattt attgattaac ttgatctaac cagccccatt
    31921 tatcttcggt ttcgccagtg aagaggccga agaaggcttg ctgaatgcgt ttggtaaccg
    31981 ggccgcaacg gccttcacca acctgaatac cgtctacact gcgcactggc gtgatttctg
    32041 ccgcagtacc ggacataaac acttcatccg ccaggtacag tgattcgcgc gacagcacct
    32101 gctcacgcac ttcaattccc agctctttcg ccagtttgat gatggcgtca cgggtaatac
    32161 ccggcagcgc ggatgaggtg aacggtgggg tgaacagcac accgtctttc acttcaaaca
    32221 ggttttcgcc tgcgccttca gagatgtaac cgttcacatc cagcgcgata ccttcctgat
    32281 aaccgtggcg gcgtgcttca ctacccacca gcagggaaga gaggtagtta ccaccggctt
    32341 ttgccgcggt tgggatggtg tttggtgctg cgcggttcca ggaggaaacc atcgcatcga
    32401 tcccctgctc cagcgcttct gcgcccagat acgcccccca cgggaaagcg gcgataatca
    32461 cgtcggttga gtatcccgct ggcgggttta cgcccatgcc aacatcacca acgaagatca
    32521 gcggacggat ataggcgctg gtgagattgt ttttgcggat cacgtcacga caagcttcca
    32581 tcagctcatc aatgctctgc gaaaccggga agcgatagat tttggcggag tcatgcagac
    32641 gctgcatatg ctcacgatgg cggaatacaa ccggtccttt gtgcgagtcg tagcaacgga
    32701 tgccttcaaa aaccgaggtg ccatagtgca gcgcgtgcga catcacatgc accttcgcgt
    32761 cttcccagcg aaccatctcc ccattgaacc aaatgtaatc agctttcttc gtggtcattt
    32821 ttatattcct tttgcgctca ggcgcggatt tgttgtgatg tggttgtgct ctggcagatg
    32881 gcaacgtgtg cgacgtccac cagtttattt aactgactaa acagtaagtc gaccgaccgt
    32941 gggctggcaa cggtcaattc gatatttata ttttgtgcat cgctggcggc ggccatattc
    33001 attgaacaga cgtggaaacc acgatgacgc accacgcgta aaacacgttc taaggtttcc
    33061 ggattgaagc gagccgatac attgacctga tgttgcatca tgataatttc tccaacattt
    33121 ctgaattact ggcaccaggc ggcaccagcg gccagacgtt ctcaagttcg tcgattgaga
    33181 catgaagcag gtatggccca tcactgttca gcatggtgtc gagtgccgct tcaacctggt
    33241 ctttatgggt gatgtgttgg ccagggatgc cgaaggcgct ggctaacatg aggaaatcgg
    33301 ggttatcggt aagggtggtt tcgctgtatc gttcctggaa aaacagttgc tgccattgtc
    33361 gaaccatccc taaccgttgg ttatcgagta agacgatttt caacggtaac tgcttgcgtt
    33421 ttacggtgcc cagctcttgc acattcatca tgaaagagcc gtcaccggag atacagacga
    33481 cagtatcgtt cggtcgcgcg acttgtgcgc caaccgccgc cggtaaaccg aaacccatgg
    33541 tgcctaagcc gctggaggtg atgaagtttt ccgggcgagt gtggacgatg tgctgggcag
    33601 cccacatctg gtgctgcccc acatctgtgg tcacgatgca atccgcagat ttacgatccg
    33661 acagttgttt taacaacaac ggcgcgtaga tagcgtcacc aggatggtcg taacgccagg
    33721 catgttcatc acgcagttgc gcgcagtgtt gctgccagtc atcgatattt aacggctgct
    33781 gtaatgctgg taacagagca tttaaatcac cctgtaatgc cacatgtgcc tgacgcagct
    33841 tgttcatttc tgccggatcg atatccatat ggataacgct ggcgtgtggt gcgaaggtgt
    33901 tcagtttgcc ggtcacccgg tcatcaaaac gtgcgcccac ggcgatcagt agatcgcact
    33961 cctgcactgc gaagttcgcc gctttggtgc cgtgcatccc cagcatgccc agatagtacg
    34021 gataatctgc ttctactgcg cccagccctt tcaacgtaca ggtggcaggc atttttgtgg
    34081 cagcgagaaa ttcacgcaaa gccgaaactg cctgcgccat gcccacgcca ccaccaacgt
    34141 acagcatcgg tttttgcgct tttgccagca tctgacgcgc ttgctcgact tcggcatgtg
    34201 ggaaagtcac ttcgttttca acagtggtga accacggttc cagatcgccg ctggctaact
    34261 ggatatcttt tggaatatcg accaaaaccg gaccaggacg accagagctg gcgacgtcaa
    34321 acgcttcggc catgatacgc ggtaactctt ccagtgattg caccaggaaa ctgtgcttgg
    34381 tgcaggctaa cgacaacccc agaatatcca cctcctgaaa tgcgtcagtg ccgataaacg
    34441 gtgcggatac ctgaccggtg atagcaacaa cggggatgga atctaacagc gcgtccgcaa
    34501 gcccggttat caggttggtt gcacctggac cagacgtggc gatgcaaacg cccgttttgc
    34561 cggtagcacg ggcataaccg atagccgcca ttgctgcacc ttgctcgtgt cggcacagca
    34621 agtgttccac gccaccgtca tacaatgcat cgtaaaccgg cataattgcg ccacccggat
    34681 aaccgaaaac ggtgttcaca ccctgtgccc gcaacgcatg taccacccac tgtgcgccat
    34741 tcatagttag ttccccgtcc tgaatcttga gaaacagaat tttgtgctgt tattcattgt
    34801 ctgctcctca gttatgtttt taaggtcaaa aaaaaccccc ggacctttcg gtgcgggggt
    34861 cttagttcgt taaggcttga tctctaagcc tttcctcgtc caagtgcagc cccgcacggt
    34921 gggataataa tcaccaccac gctaatcacg accaggctaa tcactcgtag aagggctgtc
    34981 attttgtctt ttcttgcatc ttgttcgaag gaatgcctaa agagttacca tagattttgc
    35041 gaatagcaca agatattttt tggccgattt ttatgatgtc tttaataatt tcaagagaaa
    35101 ttgttgtttt ttaaaaagaa aatagtgagt gaaaaaaatt caatttcagt atgaatgttg
    35161 ttcccttcct cgtagttcat cctttgcccg ctgcattttt gcgagcatca ttccaccgcc
    35221 agaacaactc aacttcactt gcacaaataa acaggaatca gccactaaaa ttcacgaatt
    35281 agcgggctac agcagcgata tcttatgcac ataatggtga ctcaaggagg gcttatgtca
    35341 ctgtcaattg ttcatacccg cgcagccctg ggagtaaatg cgcccccaat cactgttgag
    35401 gtacatatca gtaaaggtct accaggctta acgatggtgg gcttaccaga aacaacggta
    35461 aaagaagctc gcgatcgcgt gcgcagcgcc attatcaata gcggatatga atatccggcg
    35521 aaaaaaatca ccatcaacct tgctccagcc gatctgccaa aagaaggggg acgatatgat
    35581 ttgcctatcg ctattgcgtt actggcggcc tcagaacagc ttacagccaa taagttagat
    35641 gaatatgaat tagtcggaga actggcgctt acaggcgctc tgcgtggcgt tcccggcgca
    35701 atctccagtg caactgaagc cattaagtcg ggcagaaaaa ttatcgtcgc gaaagataac
    35761 gaggatgaag tggggctaat taacggtgaa ggatgcctga tagccgatca tctgcaggct
    35821 gtctgtgcgt ttctggaggg taagcacgct ctcgaacgcc cgaaaccaac tgatgcagta
    35881 tcccgggcgc tacaacatga tctcagtgat gttgtcggtc aggagcaagg aaagcgagga
    35941 ctggaaatta ccgccgctgg cgggcacaac cttttactga ttgggccgcc gggaacaggt
    36001 aaaacaatgc tcgccagccg tattaatggc ctgttgccag atttaagcaa tgaagaggca
    36061 ctggagagcg ctgcgatatt aagtctggta aatgctgaat cagtacaaaa acaatggcgg
    36121 cagcgcccat tccgctcacc tcatcacagt gcgtcgttaa ctgcgatggt gggcggtggt
    36181 gcaattccag ggccaggtga aatttcgctg gcgcataacg gcgtgctttt tcttgatgag
    36241 ctacctgaat ttgaacggcg tacactggat gccttgcgag agccgattga atccgggcag
    36301 atccatcttt cacgcacacg agcaaaaata acctatccag cccgtttcca acttgttgcg
    36361 gcgatgaatc ccagccctac cggacattat cagggaaacc ataaccgctg cacaccagaa
    36421 cagacattac gttatctcaa ccggctctcg gggccctttc tcgaccgctt cgatctctca
    36481 ctagagatcc cattaccgcc ccccggcatt ttgagtaaaa cggtagtgca gggagaaaac
    36541 agcaccaccg ttaaacaacg tgtaatggcc gccagagagc gccaatttaa gcggcagaat
    36601 aaactgaacg cctggctgga taatccggaa atacgccaat tctgcaagct tgagagcgaa
    36661 gatgcgcagt ggctggaaga aacgctgatc catctggggt tatcgattcg tgcctggcag
    36721 cggttattga aagttgcacg aaccattgct gatattgatc agtctgacat tatcacacgt
    36781 cagcatttgc aggaggcagt tagctatcgt gcgattgacc gtttgctcat ccatctgcaa
    36841 aaactactga cataaaaaaa agggcatttc gcccttttta ttaatcgtca gaatcggtgt
    36901 agtcttcagc accttcaacc tgcggtttac cgccggaaag ggtgtgaaaa cgttttggac
    36961 gcttgatacg cgtcatatac ttggaccaca cgcgttctgc ttctgtcact ggctcacgtt
    37021 cgccacggca tactgctacg aagagtttct cttcctcggt aaccggctcg cgtttgccaa
    37081 gatccaactc attgaaggca taaccatgac gctcaagcag ttgtgcctct ttgatggtga
    37141 agtcaccatg acgagagaat ccacgtggat aatgtttatt gtcgaaatat cgattagtcg
    37201 tcgtaaagct ttccgccatc ctgcacgctc ctaattcttt gaccgagcta gttatggcgc
    37261 ggagtattag ttacgcttga cagagtgtaa aacaaaacat ttaaatcata acgacaaata
    37321 attttgcgga gagcactgtg gatacggaat tgttaaaaac tttcctggaa gttagccgaa
    37381 cgcgtcactt tggtcgagca gctgaatcgc tctatctgac ccaatcagcc gtgagctttc
    37441 gaatcagaca actggaaaat caactgggtg tgaacctttt cacccgccac agaaacaata
    37501 tccgtttaac cgctgctggt gaaaaactac tgccttatgc agaaacgctc atgagcacct
    37561 ggcaggccgc ccgtaaggag gtggcgcata cctcacgaca taacgagttt tctatcggtg
    37621 ccagcgcctc gttgtgggaa tgtatgctta atcagtggct gggacgcttg tatcaaaatc
    37681 aggatgtcca tacaggctta cagttcgaag cgcgaattgc ccaacggcag tctctggtaa
    37741 aacagctgca tgaacgccag cttgatcttc ttattactac tgaagcgccc aaaatggacg
    37801 aatttagcag tcagttgctc gggtatttca ctttagcgct ttataccagt gccccttcta
    37861 aactaaaggg agatcttaac tatctgcgac tggaatgggg gccagatttt caacagcatg
    37921 aggcaggttt gatcggtgct gacgaagttc ccattctgac aaccagttct gctgaactgg
    37981 cacagcaaca gattgcgacg cttaatggtt gcacctggct acccgtcagt tgggcgcgta
    38041 aaaagggcgg cctgcatacc gttgtcgata gcacaacact ttcacggccg ctttatgcca
    38101 tatggctgca aaatagcgat aaaaatgcgt tgattcgcga tctattgaaa attaacgtgc
    38161 tggatgaagt gtattaatct gaatggctgg caaggatgcc ggtagaagga tttacttcgg
    38221 agaggttatt tcagataaaa aaaatcctta gctttcgcta aggatgattt ctggcagggg
    38281 cggagagact cgaactccca acacccggtt ttggagaccg gtgctctacc aattgaacta
    38341 cgcccctaat tagggtggcg gaacggacgg gactcgaacc cgcgaccccc tgcgtgacag
    38401 gcaggtattc taaccgactg aactaccgct ccaccgaatt cttttacaac caccggtttt
    38461 atgaccggct tactgcttaa tttgatgcct ggcagttccc tactctcgca tggggagacc
    38521 ccacactacc atcggcgcta cggcgtttca cttctgagtt cggcatgggg tcaggtggga
    38581 ccaccgcgct acggccgcca ggcaaattct gtttcatcag accgcttctg cgttctgatt
    38641 taatctgtat caggctgaaa atc
//
LOCUS       DAZFLD010000014        36223 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.014, whole genome shotgun sequence.
ACCESSION   DAZFLD010000014 DAZFLD010000000
VERSION     DAZFLD010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 36223)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36223)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36223
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.014"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            141..1514
                     /gene="aroP"
                     /locus_tag="ACHKBL_000678"
     CDS             141..1514
                     /gene="aroP"
                     /locus_tag="ACHKBL_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter AroP"
                     /protein_id="HFO5817593.1"
                     /translation="MMEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSA
                     GPGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVL
                     YVLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVKVFGEMEFWFAIIK
                     VIAVVAMIIFGGWLLFSGNGGPQASVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLE
                     LVGITAAEADNPEQSIPKATNQVIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIF
                     HELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPV
                     NTILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWAMISLAHMKFRRAKQEQG
                     VVTCFPALLYPLGNWICLLFMAAVLVIMLMTPGMAISVYLIPVWLIVLGIGYLFKEKT
                     AKAVKAH"
     gene            complement(1557..2411)
                     /gene="ampE"
                     /locus_tag="ACHKBL_000679"
     CDS             complement(1557..2411)
                     /gene="ampE"
                     /locus_tag="ACHKBL_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase regulator AmpE"
                     /protein_id="HFO5817594.1"
                     /translation="MTLFTTLLVLIFERLFKLGEHWQIDHRLEAFFRRVKHFSLGRTL
                     GMTIIAMGVTFLLLRALQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLTAASRND
                     SHARATMAGELTMIHGVPAGCDEREYLRELQNALLWINFRFYLAPLFWLIVGGTWGPV
                     TLMGYAFLRAWQYWLARYQTPHHRLQSGIDAVLHVLDWVPVRLAGVVYALIGHGEKAL
                     PAWFASLGDFHTSQYQVLTRLAQFSLAREPHVDKVETPKAAVSMAKKTSFVVLVVIAL
                     LTIYGALV"
     gene            complement(2408..2959)
                     /gene="ampD"
                     /locus_tag="ACHKBL_000680"
     CDS             complement(2408..2959)
                     /gene="ampD"
                     /locus_tag="ACHKBL_000680"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HFO5817595.1"
                     /translation="MLLEQGWLVGARRVPSPHYDCRPDDETPTLLVVHNISLPPGEFG
                     GPWIDALFTGTIDPQAHPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQY
                     QGRERCNDFSIGIELEGTDTLAYTDAQYQQLVAVTRALIDRYPDIANNMTGHCDIAPD
                     RKTDPGPAFDWARFRALVSKETT"
     gene            3047..3940
                     /gene="nadC"
                     /locus_tag="ACHKBL_000681"
     CDS             3047..3940
                     /gene="nadC"
                     /locus_tag="ACHKBL_000681"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HFO5817596.1"
                     /translation="MPPRRYNPDTRRDELLERINLDIPGAVAQALREDLGGTVDANND
                     ITAKLLPENSRSHATVITRENGVFCGKRWVEEVFIQLAGDDVTIIWHVDDGDVINANE
                     PLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQLLDTRKTLPGLR
                     SALKYAVLCGGGANHRLGLSDAFLIKENHIIASGSVRQAVEKASWLHPDAPVEVEVEN
                     LEELDEALKAGADIIMLDNFETEQMREAVKRTNGKALLEVSGNVTDKTLREFAETGVD
                     FISVGALTKHVQALDLSMRFR"
     gene            4143..4583
                     /gene="ppdD"
                     /locus_tag="ACHKBL_000682"
     CDS             4143..4583
                     /gene="ppdD"
                     /locus_tag="ACHKBL_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HFO5817597.1"
                     /translation="MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQ
                     TFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTRYVSAMSVAKGVVSLTGQESLNG
                     LSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN"
     gene            4593..5978
                     /gene="gspE"
                     /locus_tag="ACHKBL_000683"
     CDS             4593..5978
                     /gene="gspE"
                     /locus_tag="ACHKBL_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspE"
                     /protein_id="HFO5817598.1"
                     /translation="MNIPQLTALCLRYQGVLLDASEEVVHVAVVDAPSHELLDALHFA
                     TTKRIEITCWTRQQMEGHASRTQQTLPVAIHEKHQPKAELLTRTLQSALEQRASDIHI
                     EPADNAYRIRLRIDGVLHPLPDVLPDAGVALTARLKVLGNLDIAEHRLPQDGQFTVEL
                     AGNAISFRIATLPCRGGEKVVLRLLQQVSQALDVNTLGMQPLQLADFAHALQQPQGLV
                     LVTGPTGSGKTVTLYSALQTLNTADINICSVENPVEIPIAGLNQTQIHPRAGLTFQGV
                     LRALLRQDPDVIMIGEIRDGETAEIAINAAQTGHLVLSTLHTNSTCETLVRLQQMGVA
                     RWMLSSALTLVIAQRLVRKLCPHCRRQQGEPIHIPDNVWPSPLPRWQAPGCVHCYHGF
                     YGRTALFEVLPITPVIRQLISANTDVESLETHARQAGMRTLFENGCLAVEQGLTTFEE
                     LIRVLGMPHGE"
     gene            5968..7170
                     /gene="hofC"
                     /locus_tag="ACHKBL_000684"
     CDS             5968..7170
                     /gene="hofC"
                     /locus_tag="ACHKBL_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein transport protein HofC"
                     /protein_id="HFO5817599.1"
                     /translation="MASKQLWRWRGITGDGNAQDGMLWAESRALLLMALQQQMVTPLS
                     LKRIAINSAQWRGDKSAEVIHQLATLLKAGLTLSEGLALLAEQHPSKQWQALLQSLAH
                     DLEQGVAFSNALFPWSEVFPPLYQAMIRTGELTGKLDECCFELARQQKAQRQLTDKVK
                     SALRYPIIILAMAIMVVVAMLHFVLPEFAAIYKTFNTPLPVLTQGIMTLADFSGEWGW
                     LLVLFGFLLAIANKLLMRRPTWLIARQKLLLRIPIMGSLMRGQKLTQIFTILALTQSA
                     GITFLQGVESVRETMRCPYWVQLLTQIQHDISNGQPIWLALKNAGEFSPLCLQLVRTE
                     EASGSLDLMLDNLAHHHRDNTMALADNLAALLEPALLIITGGIIGTLVVAMYLPIFHL
                     GDAMSGMG"
     gene            complement(7205..8248)
                     /gene="guaC"
                     /locus_tag="ACHKBL_000685"
     CDS             complement(7205..8248)
                     /gene="guaC"
                     /locus_tag="ACHKBL_000685"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902560 - GMP reductase complex [Evidence
                     IEA];
                     GO_function: GO:0003920 - GMP reductase activity [Evidence
                     IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HFO5817600.1"
                     /translation="MRIEEDLKLGFKDVLIRPKRSTLKSRSDVELERQFTFKHSGQSW
                     SGVPIIAANMDTVGTFSMASALASFDILTAVHKHYSFEEWQAFINNSSADVLKHVMVS
                     TGTSDADFEKTKQILDLNPALNFVCIDVANGYSEHFVQFVAKAREAWPTKTICAGNVV
                     TGEMCEELILSGADIVKVGIGPGSVCTTRVKTGVGYPQLSAVIECADAAHGLGGMIVS
                     DGGCTTPGDVAKAFGGGADFVMLGGMLAGHEESGGRIVEENGEKFMLFYGMSSESAMK
                     RHVGGVAEYRAAEGKTVKLPLRGPVENTARDILGGLRSACTYVGASRLKELTKRTTFI
                     RVLEQENRIFNNL"
     gene            complement(8404..8448)
                     /locus_tag="ACHKBL_000686"
     CDS             complement(8404..8448)
                     /locus_tag="ACHKBL_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5817601.1"
                     /translation="MIIKTLKMSARKRN"
     gene            8473..9093
                     /gene="coaE"
                     /locus_tag="ACHKBL_000687"
     CDS             8473..9093
                     /gene="coaE"
                     /locus_tag="ACHKBL_000687"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308134.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HFO5817602.1"
                     /translation="MRYIVALTGGIGSGKSTVANAFADLGINVIDADIIARQVVEPGA
                     PALHAIADHFGANMIAADGTLQRRALRERIFANPEEKNWLNALLHPLIQQETQHQIQQ
                     ATSPYVLWVVPLLVENSLYKKANRVLVVDVSPETQLKRTMQRDDVTREHVEQILAAQA
                     PREARLAVADDVIDNNGAPDAIASDVARLHAHYLQLASQFVSQEKP"
     gene            9093..9836
                     /gene="zapD"
                     /locus_tag="ACHKBL_000688"
     CDS             9093..9836
                     /gene="zapD"
                     /locus_tag="ACHKBL_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HFO5817603.1"
                     /translation="MQTQVLFEHPLNEKMRTWLRIEFLIQQLTVNLPVVDYAGALHFF
                     RNVSELLDVFERGEVRTELLKELDRQQRKLQTWIGVPGVDQSRIEALIQQLKAAGSVL
                     ISAPRIGQFLREDRLIALVRQRLSIPGGCCSFDLPTLHIWLHLPQAQRDSQVETWIAS
                     LNPLTQALTMVLDLIRQSAPFRKQTSLNGFYQDNGGDADLLRLNLSLDSQLYPQISGH
                     KSRFAIRFMPLDSENGQVPERLDFELACC"
     gene            9846..10043
                     /gene="yacG"
                     /locus_tag="ACHKBL_000689"
     CDS             9846..10043
                     /gene="yacG"
                     /locus_tag="ACHKBL_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HFO5817604.1"
                     /translation="MSETITVNCPTCGKTVVWGEISPFRPFCSKRCQLIDLGEWAAEE
                     KRIPSSGDLSESDDWSEEPKQ"
     gene            complement(10245..10643)
                     /gene="mutT"
                     /locus_tag="ACHKBL_000690"
     CDS             complement(10245..10643)
                     /gene="mutT"
                     /locus_tag="ACHKBL_000690"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HFO5817605.1"
                     /translation="MKKLQIAVGIIRNENNEIFITRRAADAHMANKLEFPGGKIEMGE
                     TPEQAVVRELQEEVGITPQHLSLFEILEYEFPDRHITLWFWLVESWEGEPWGKEGQPG
                     EWMSLVGLNADDFPPANEPVLAKLKRVYVG"
     gene            complement(10703..13408)
                     /gene="secA"
                     /locus_tag="ACHKBL_000691"
     CDS             complement(10703..13408)
                     /gene="secA"
                     /locus_tag="ACHKBL_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HFO5817606.1"
                     /translation="MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELK
                     GKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEM
                     RTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPG
                     MPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDE
                     ARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTE
                     RGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVI
                     IVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTG
                     TADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQ
                     PVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAG
                     RGTDIVLGGSWQAEVAALENPTAEQIEKIKADWQVRHDAVLAAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKPGEAIEHPWVT
                     KAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIRED
                     VFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPIAEWLDKEPELHEETLRERIL
                     AQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKD
                     PKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQ
                     LSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ"
     gene            complement(13470..13982)
                     /gene="secM"
                     /locus_tag="ACHKBL_000692"
     CDS             complement(13470..13982)
                     /gene="secM"
                     /locus_tag="ACHKBL_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308128.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secA translation cis-regulator SecM"
                     /protein_id="HFO5817607.1"
                     /translation="MSGILTRWRQFGKRYFWPHLLLGMVAASLGLPALSNAAEPNAPA
                     KATTRNHEPSAKVNFGQLALLEANTRRPNSNYSVDYWHQHAIRTVIRHLSFAMAPQTL
                     PVAEESLPLQAQHLALLDTLSALLTQEGTPSEKGYRIDYAHFTPQAKFSTPVWISQAQ
                     GIRAGPQRLS"
     gene            complement(14213..15130)
                     /gene="lpxC"
                     /locus_tag="ACHKBL_000693"
     CDS             complement(14213..15130)
                     /gene="lpxC"
                     /locus_tag="ACHKBL_000693"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HFO5817608.1"
                     /translation="MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDL
                     NPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIP
                     IMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVRVEDGDKWAEFKPYNGFSLDFTID
                     FNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVV
                     DDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFTAYKSGHALNNKLLQAVL
                     AKQEAWEYVTFQDDAELPLAFKAPSAVLA"
     gene            complement(15231..16382)
                     /gene="ftsZ"
                     /locus_tag="ACHKBL_000694"
     CDS             complement(15231..16382)
                     /gene="ftsZ"
                     /locus_tag="ACHKBL_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HFO5817609.1"
                     /translation="MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNT
                     DAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAA
                     GMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSL
                     ITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMS
                     EMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFE
                     TVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQVQQP
                     VMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD"
     gene            complement(16443..17705)
                     /gene="ftsA"
                     /locus_tag="ACHKBL_000695"
     CDS             complement(16443..17705)
                     /gene="ftsA"
                     /locus_tag="ACHKBL_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HFO5817610.1"
                     /translation="MIKATDRKLVVGLEIGTAKVAALVGEVLPDGMVNIIGVGSCPSR
                     GMDKGGVNDLESVVKCVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVPISEE
                     EVTQEDVENVVHTAKSVRVRDEHRVLHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHL
                     ITCHNDMAKNIVKAVERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDI
                     AVYTGGALRHTKVIPYAGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVE
                     VPSVGGRPPRSLQRQTLAEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVL
                     TGGAAQIEGLAACAQRVFHTQVRIGAPLNITGLTDYAQEPYYSTAVGLLHYGKESHLN
                     GEAEVEKRVTASVGSWIKRLNSWLRKEF"
     gene            complement(17702..18532)
                     /gene="ftsQ"
                     /locus_tag="ACHKBL_000696"
     CDS             complement(17702..18532)
                     /gene="ftsQ"
                     /locus_tag="ACHKBL_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HFO5817611.1"
                     /translation="MSQAALNTRNSEEEVSSRRNNGTRLAGILFLLTVLTTVLVSGWV
                     VLGWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQR
                     LPWIKQVSVRKQWPDELKIHLVEYVPIARWNDQHMVDAEGNTFSVPPDRTSKQVLPML
                     YGPEGSANEVLQGYREMGQMLAKDRFTLKEAAMTARRSWQLTLNNDIKLNLGRGDTMK
                     RLARFVELYPVLQQQAQTDGKRISYVDLRYDSGAAVGWAPLPPEESTQQQNQAQAEQQ
                     "
     gene            complement(18534..19454)
                     /gene="ddlB"
                     /locus_tag="ACHKBL_000697"
     CDS             complement(18534..19454)
                     /gene="ddlB"
                     /locus_tag="ACHKBL_000697"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFO5817612.1"
                     /translation="MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDANPVDPKE
                     VDVTQLKSMGFQKVFIALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSMDKLRSKL
                     LWQGAGLPVAPWVALTRAEFEKGLSDKQLAEISALGLPVIVKPSREGSSVGMSKVVAE
                     NALQDALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSIRIQPSGTFYDYEAKYLS
                     DETQYFCPAGLEASQEANLQALVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPG
                     MTSHSLVPMAARQAGMSFSQLVVRILELAD"
     gene            complement(19447..20922)
                     /gene="murC"
                     /locus_tag="ACHKBL_000698"
     CDS             complement(19447..20922)
                     /gene="murC"
                     /locus_tag="ACHKBL_000698"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HFO5817613.1"
                     /translation="MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGY
                     QISGSDLAPNPVTQQLMNLGATIYFNHRPENVRDASVVVVSSAISADNPEIVAAHEAR
                     IPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAG
                     VHARLGHGRYLIAEADESDASFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFL
                     HNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFSEDADVRVEDYQQIGPQGHFTLLR
                     QDKEPMRVTLNAPGRHNALNAAAAVAVATEEGIDDEAILRALESFQGTGRRFDFLGEF
                     PLEPVNGKSGTAMLVDDYGHHPTEVDATIKAARAGWPDKNLVMLFQPHRFTRTRDLYD
                     DFANVLTQVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDPILVPDPAQVAEML
                     APVLTGNDLILVQGAGNIGKIARSLAEIKLKPQTPEEEQHD"
     gene            complement(20976..22043)
                     /gene="murG"
                     /locus_tag="ACHKBL_000699"
     CDS             complement(20976..22043)
                     /gene="murG"
                     /locus_tag="ACHKBL_000699"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HFO5817614.1"
                     /translation="MSGQGKRLMVMAGGTGGHVFPGLAVAHHLMAQGWQVRWLGTADR
                     MEADLVPKHGIEIDFIRISGLRGKGIKALIAAPLRIFNAWRQARAIMKAYKPDVVLGM
                     GGYVSGPGGLAAWSLGIPVVLHEQNGIAGLTNKWLAKIATKVMQAFPGAFPNAEVVGN
                     PVRTDVLALPLPQQRLAGREGPVRVLVVGGSQGARILNQTMPQVAAKLGDSVTIWHQS
                     GKGSQQSVEQAYAEAGQPQHKVTEFIDDMAAAYAWADVVVCRSGALTVSEIAAAGLPA
                     LFVPFQHKDRQQYWNALPLEKAGAAKIIEQPQLSVDAVANTLAGWSRETLLTMAERAR
                     AASIPDATERVANEVSRAARA"
     gene            complement(22040..23284)
                     /gene="ftsW"
                     /locus_tag="ACHKBL_000700"
     CDS             complement(22040..23284)
                     /gene="ftsW"
                     /locus_tag="ACHKBL_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HFO5817615.1"
                     /translation="MRLSLPRLKMPRLPGFSILVWISTALKGWVMGSREKDTDSLIMY
                     DRTLLWLTFGLAAIGFIMVTSASMPIGQRLTNDPFFFAKRDGVYLILAFILAIITLRL
                     PMEFWQRYSATMLLGSIILLMIVLVVGSSVKGASRWIDLGLLRIQPAELTKLSLFCYI
                     ANYLVRKGDEVRNNLRGFLKPMGVILVLAVLLLAQPDLGTVVVLFVTTLAMLFLAGAK
                     LWQFIAIIGMGISAVVLLILAEPYRIRRVTAFWNPWEDPFGSGYQLTQSLMAFGRGEL
                     WGQGLGNSVQKLEYLPEAHTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKAL
                     EIDHRFSGFLACSIGIWFSFQALVNVGAAAGMLPTKGLTLPLISYGGSSLLIMSTAIM
                     MLLRIDYETRLEKAQAFVRGSR"
     gene            complement(23284..24600)
                     /gene="murD"
                     /locus_tag="ACHKBL_000701"
     CDS             complement(23284..24600)
                     /gene="murD"
                     /locus_tag="ACHKBL_000701"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HFO5817616.1"
                     /translation="MADYQGKNVVIIGLGLTGLSCVDFFLARGVTPRVMDTRMTPPGL
                     DKLPEAVERHTGGLNDEWLMAADLIVASPGIALAHPSLSAAADAGIEIVGDIELFCRE
                     AQAPIVAVTGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDDECELYVLELS
                     SFQLETTSSLQAVAATILNVTEDHMDRYPFGLQQYRAAKLRIYENAKICVVNADDALT
                     MPIRGADERCVSFGVNMGDYHLNHQQGETWLRVKGEKVLNVKEMKLSGQHNYTNALAA
                     LALADAAGLPRASSLKALTTFTGLPHRFEVVLEHNGVRWVNDSKATNVGSTEAALNGL
                     HVDGTLHLLLGGDGKSADFSPLARYLNGDNVRLYCFGRDGAQLAALRPEVAEQTETME
                     QAMRLLAPRVQPGDMVLLSPACASLDQFKNFEQRGNEFARLAKELG"
     gene            complement(24603..25685)
                     /gene="mraY"
                     /locus_tag="ACHKBL_000702"
     CDS             complement(24603..25685)
                     /gene="mraY"
                     /locus_tag="ACHKBL_000702"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HFO5817617.1"
                     /translation="MLVWLAEHLVKYYSGFNVFSYLTFRAIVSLLTALFISLWMGPRM
                     IAHLQKLSFGQVVRNDGPESHFSKRGTPTMGGIMILTAIVISVLLWAYPSNPYVWCVL
                     VVLVGYGVIGFVDDYRKVVRKDTKGLIARWKYFWMSVIALGVAFALYLVGKDTPATQL
                     VVPFFKDVMPQLGLFYILLAYFVIVGTGNAVNLTDGLDGLAIMPTVFVAGGFALVAWA
                     TGNMNFASYLHIPYLRHAGELVIVCTAIVGAGLGFLWFNTYPAQVFMGDVGSLALGGA
                     LGIIAVLLRQEFLLVIMGGVFVVETLSVILQVGSFKLRGQRIFRMAPIHHHYELKGWP
                     EPRVIVRFWIISLMLVLIGLATLKVR"
     gene            complement(25679..27037)
                     /gene="murF"
                     /locus_tag="ACHKBL_000703"
     CDS             complement(25679..27037)
                     /gene="murF"
                     /locus_tag="ACHKBL_000703"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HFO5817618.1"
                     /translation="MISVTLSQLTDILNGELKGADITLDAVTTDTRKLTPGCLFVALK
                     GERFDAHDFADQAKAGGAGALLVNRPLDIDLPQVIVKDTRLAFGELAAWVRQQVPARV
                     VALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTPEYDYAVIELG
                     ANHQGEIAWTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMN
                     ADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGTEFTLQTPTGSVDVLLP
                     LPGRHNIANALAAAALSMSVGATLDAIKAGLANLKAVPGRLFPIQLAENQLLLDDSYN
                     ANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEACHIQVGEAAKAAGIDRVLSVGK
                     QSHAISTASGVGEHFSDKTALIARLKSLIAEQQVITILVKGSRSAAMEEVVRALQENG
                     TC"
     gene            complement(27034..28521)
                     /gene="murE"
                     /locus_tag="ACHKBL_000704"
     CDS             complement(27034..28521)
                     /gene="murE"
                     /locus_tag="ACHKBL_000704"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HFO5817619.1"
                     /translation="MADRNLRDLLAPWVPDAPSRALREMTLDSRVAAAGDLFVAVVGH
                     QADGRRYIPQAIAQGVAAIIAEAKDEATDGEIREMHGVPVIYLSQLNERLSALAGRFY
                     HEPSDNLRLVGVTGTNGKTTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENTTG
                     SAVDVQHELAGLVDQGATFCAMEVSSHGLVQHRVAALKFAASVFTNLSRDHLDYHGDM
                     EHYEAAKWLLYSEHHCGQAIINADDEVGRRWLAKLPDAVAVSMEDHINPNCHGRWLKA
                     TEVNYHDSGATIRFSSSWGDGEIESHLMGAFNVSNLLLALATLLALGYPLADLLKTAA
                     RLQPVCGRMEVFTAPGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVFGCGGDRDK
                     GKRPLMGAIAEEFADVAVVTDDNPRTEEPRAIINDILAGMLDAGHAKVMEGRAEAVTC
                     AVMQAKENDVVLVAGKGHEDYQIVGNQRLDYSDRVTVARLLGVIA"
     gene            complement(28508..30274)
                     /gene="ftsI"
                     /locus_tag="ACHKBL_000705"
     CDS             complement(28508..30274)
                     /gene="ftsI"
                     /locus_tag="ACHKBL_000705"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase FtsI"
                     /protein_id="HFO5817620.1"
                     /translation="MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAW
                     LQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAG
                     GISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNPDMAEYIKKLKLPGIH
                     LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVI
                     EDISSTDSQAAHNLALSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMA
                     NSPSYNPNNLSGTPKEAMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTVPY
                     RINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALAMPSSALVDTYSRFGLGKATNLG
                     LVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVD
                     PPVPGERVFPESIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPDGRYI
                     NKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRTMNIEPD
                     ALTTGDKNEFVINQGEGTGGRS"
     gene            complement(30290..30655)
                     /gene="ftsL"
                     /locus_tag="ACHKBL_000706"
     CDS             complement(30290..30655)
                     /gene="ftsL"
                     /locus_tag="ACHKBL_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HFO5817621.1"
                     /translation="MISRVTEALSKVKGSMGSHERHALPGVIGDDLLRFGKLPLCLFI
                     CIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIAT
                     EKLQMQHVDPSQENIVVQK"
     gene            complement(30652..31593)
                     /gene="rsmH"
                     /locus_tag="ACHKBL_000707"
     CDS             complement(30652..31593)
                     /gene="rsmH"
                     /locus_tag="ACHKBL_000707"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HFO5817622.1"
                     /translation="MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQ
                     LGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDL
                     GVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEERF
                     AKRIARAIVERNREQPMTRTKELAEVVAAATPVKDKFKHPATRTFQAVRIWVNSELEE
                     IEQALKSSLNVLAPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKL
                     GGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA"
     gene            complement(31595..32053)
                     /gene="mraZ"
                     /locus_tag="ACHKBL_000708"
     CDS             complement(31595..32053)
                     /gene="mraZ"
                     /locus_tag="ACHKBL_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HFO5817623.1"
                     /translation="MFRGATLVNLDSKGRLSVPTRYREQLLENAAGQMVCTIDIHHPC
                     LLLYPLPEWEIIEQKLSRLSSMNPVERRVQRLLLGHASECQMDGAGRLLIAPVLRQHA
                     GLTKEVMLVGQFNKFELWDETTWHQQVKEDIDAEQLATGDLSERLQDLSL"
     gene            complement(32655..33659)
                     /gene="cra"
                     /locus_tag="ACHKBL_000709"
     CDS             complement(32655..33659)
                     /gene="cra"
                     /locus_tag="ACHKBL_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009750 - response to fructose [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite repressor/activator"
                     /protein_id="HFO5817624.1"
                     /translation="MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVRE
                     HNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQ
                     PDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANDPFPIVALDRALDREHFTSVV
                     GADQDDAEMLAEELRKFPAETVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYAN
                     SYEREAAAQLFEKWLETHPMPQALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDN
                     ELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTRIKRNLYRRGVLSRS
                     "
     gene            complement(33741..33827)
                     /locus_tag="ACHKBL_000710"
     CDS             complement(33741..33827)
                     /locus_tag="ACHKBL_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fruR/shl operon leader peptide"
                     /protein_id="HFO5817625.1"
                     /translation="MRNLQPNMSRWAFFAKSVGTWNKSSCRS"
     gene            complement(33839..34330)
                     /gene="ilvN"
                     /locus_tag="ACHKBL_000711"
     CDS             complement(33839..34330)
                     /gene="ilvN"
                     /locus_tag="ACHKBL_000711"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HFO5817626.1"
                     /translation="MRRILSVLLENESGALSRVIGLFSQRGYNIESLTVAPTDDPTLS
                     RMTIQTVGDEKVLEQIEKQLHKLVDVLRVSELGQGAHVEREIMLVKIQASGYGRDEVK
                     RNTEIFRGQIIDVTPSLYTVQLAGTSDKLDAFLASIRDVAKIVEVARSGVVGLSRGDK
                     IMR"
     gene            complement(34333..36057)
                     /gene="ilvI"
                     /locus_tag="ACHKBL_000712"
     CDS             complement(34333..36057)
                     /gene="ilvI"
                     /locus_tag="ACHKBL_000712"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HFO5817627.1"
                     /translation="MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGID
                     HVLVRHEQAAVHMADGLARATGEVGVVLVTSGPGATNAITGIATAYMDSIPLVVLSGQ
                     VATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
                     LPKDILNPANKLPYVWPESVSMRSYNPTTSGHKGQIKRALQTLVAAKKPVVYVGGGAI
                     TAGCHQQLKETVEALNLPVVSSLMGLGAFPATHRQALGMLGMHGTYEANMTMHNADVI
                     FAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVTADIPIVGDARQVLEQMLEL
                     LSQESVHQPLDEIRDWWQQIEQWRARQCLKYDTHSEKIKPQAVIETLWRLTNGDAYVT
                     SDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGS
                     IQMNIQELSTALQYELPVLVVNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRLA
                     EAYGHVGIQISHPQELESKLSEALEQVRNNRLVFVDVTVDGSEHVYPMQIRGGGMDEM
                     WLSKTERT"
     gene            36176..36220
                     /locus_tag="ACHKBL_000713"
     CDS             36176..36220
                     /locus_tag="ACHKBL_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5817628.1"
                     /translation="MKRNDKILHYRGLN"
BASE COUNT         8664 a        10158 c         8903 g         8498 t
ORIGIN      
        1 cattatcttg tgatagttaa aagtagcatt atcataattc agttacatga aacgtaaaga
       61 aatcaataca acaaacggaa ttgcaaactt acacacgcat cactgcgtag atcaaaaaaa
      121 caatcaccgc acgaggtttc atgatggaag gtcaacagca cggcgagcag ctaaagcgcg
      181 gtcttaaaaa ccgccatatt cagcttatcg cgctgggtgg cgcgataggg accgggctat
      241 tcctgggtag cgcctccgta atacagtccg cagggccagg gattatcctg ggttacgcca
      301 ttgctggttt tatcgccttt ctgatcatgc gtcagctagg tgaaatggtg gtcgaagaac
      361 ctgtcgcagg ctcctttagc cactttgctt ataaatactg gggcagtttt gccggtttcg
      421 cctctggctg gaactactgg gtactgtacg ttttagttgc catggctgag ctgactgccg
      481 tgggtaaata cattcagttc tggtatccgg aaatcccaac ctgggtttct gccgccgtgt
      541 tctttgtggt gattaacgcc atcaacctga ccaacgttaa agtgtttggc gagatggagt
      601 tctggtttgc cattatcaaa gttatcgcgg tggtagcgat gatcatcttc ggcggctggc
      661 tgctattcag cggcaacggc ggcccgcagg cgagcgttag caacctgtgg gatcagggtg
      721 gtttcctgcc gcacggcttc accgggctgg tgatgatgat ggcgattatc atgttctctt
      781 tcggcggtct ggaactggtg gggatcaccg cagcagaagc tgataatccg gagcaaagta
      841 tcccgaaagc aactaaccag gttatctacc gcatcctgat tttctatatt ggttcgttag
      901 ccgttctgct ctcgctgatg ccatggaccc gcgttactgc tgataccagt ccgtttgtac
      961 tgatcttcca cgaattgggc gatacctttg tggctaatgc gctgaacatc gtggtactga
     1021 ctgctgcgct ctccgtgtac aacagctgcg tatattgcaa cagccgtatg ctgtttggtc
     1081 tggcacaaca gggcaacgcg ccaaaagcgc tggcgtctgt cgacaaacgc ggtgtaccgg
     1141 ttaacactat tctggtgtct gcgctggtta cggcattgtg cgtgctgatt aactaccttg
     1201 caccggaatc cgcattcgga ctgttaatgg cactggtggt atctgcactg gtaatcaact
     1261 gggcgatgat tagcctggcg catatgaaat tccgtcgcgc caagcaggaa caaggcgtgg
     1321 taacttgctt ccctgctctg ctttatccgc tgggtaactg gatctgcctg ctgtttatgg
     1381 cggcggtact ggtgattatg ctgatgaccc caggaatggc gatttcggta tacctgatcc
     1441 cggtatggct gatcgtgtta ggtatcggct atctgtttaa agagaaaacc gccaaagccg
     1501 taaaagcgca ttaatctctc tacgccctca cccgtgcagg gtgagggcaa taatctttac
     1561 accaacgccc cgtaaatcgt cagtagcgca attaccacca ggaccacgaa cgaggttttc
     1621 ttcgccatgg aaaccgctgc cttcggcgtc tccaccttat cgacatgcgg ttcacgcgcc
     1681 agagagaact gtgccagacg tgttaatacc tgatattgcg aagtatggaa atcacccagc
     1741 gaagcaaacc aggccggtaa cgctttctcc ccatgaccga tcaaggcata taccacaccc
     1801 gcaagacgaa ccggcaccca atccagtaca tgaagcacgg catcaatgcc ggactgtaaa
     1861 cgatgatgcg gcgtctggta acgcgccagc cagtattgcc atgcacgcaa aaacgcatac
     1921 cccatcagcg taacgggtcc ccaggttccc cccacaatca gccagaacag cggtgcaaga
     1981 taaaaacgaa agttaatcca cagcaatgca ttttgcagct cacgcaaata ctcacgttca
     2041 tcgcagcctg ccgggacgcc gtgaatcatg gtgagttcgc cagccatcgt ggcacgggca
     2101 tggctatcat tacgtgaagc agctgtcaga taagcatgat aatgaagacg aactttacct
     2161 gcgccaatac acagcaaacc aatcagcagc cacaccagca gcgtgggaac gttgaacaat
     2221 actccctgca atgcgcgtaa cagtaaaaaa gtcacgccca tcgcaataat ggtcatgcct
     2281 aatgtgcgcc cgagagaaaa atgtttcacc cgccgaaaga acgcttcaag acgatgatca
     2341 atctgccagt gctcgcccaa cttaaacagg cgctcgaaaa ttaacaccag taaggttgta
     2401 aatagcgtca tgttgtctcc ttgctgacca gtgcacgaaa ccgtgcccaa tcaaatgcag
     2461 ggccgggatc ggttttccga tccggcgcaa tatcacaatg gcccgtcatg ttattagcga
     2521 tatctggata gcgatcaatc agtgcccgcg taaccgccac aagctgttga tactgcgcat
     2581 cggtatacgc cagcgtatcg gtgccttcca gctcaatccc aatagaaaaa tcattgcagc
     2641 gttcgcgccc ctgatactga gagactccgg catgccaggc acgtttatcg aaaggaacat
     2701 actggactat ttcaccatcg cggcgaatca aacagtgagc ggagacgcgc aaatgggcga
     2761 tctcagcaaa gaaaggatgc gcctgcggat caatagttcc agtgaataat gcgtcgatcc
     2821 acggaccgcc aaactcgcct ggcggcaggc taatattgtg caccaccagc agggtgggtg
     2881 tttcgtcatc cgggcggcaa tcgtaatgtg gtgagggaac gcggcgcgcg ccaaccagcc
     2941 acccctgttc taacaacatg caggatctcc ttatatgtgg tgctaatacc cggttcagag
     3001 tagcatgttt ctaccttatg attcgttagc tatctggagt tttaacatgc cgcctcgccg
     3061 ctataaccct gacacccgac gtgacgagct gctggaacgc attaatctcg atatccccgg
     3121 cgcggtggcc caggcgctgc gggaagattt aggcggaaca gtcgatgcca acaatgacat
     3181 tacggcaaaa cttttaccgg aaaactctcg ctctcatgcc acagtgatca cacgcgagaa
     3241 tggcgtattt tgcggcaaac gctgggttga agaggtgttt attcaactgg caggcgacga
     3301 tgtcaccata atctggcatg tggatgacgg cgatgtcatc aacgccaatg aacccttgtt
     3361 cgaacttgaa ggcccatccc gcgtgctgtt aacgggcgaa cgcactgcac ttaattttgt
     3421 gcaaaccctt tcaggggttg ccagtaaggt acgccactat gtcgaattac tggagggcac
     3481 caacacgcag ttgttggata cgcgcaaaac cttacccggc ctgcgttcag ctctgaaata
     3541 cgcggtactt tgcggcggcg gagcgaatca ccgcctgggg ctttctgatg ccttcctgat
     3601 caaagaaaac catattattg cctccggctc agtgcgccag gcggtcgaaa aagcgtcctg
     3661 gctgcaccca gatgcgccag tagaagtcga agtagagaat ctggaagaac ttgatgaagc
     3721 cctgaaagca ggtgccgata tcatcatgct ggataacttc gaaacagaac agatgcgcga
     3781 agccgtcaaa cgcaccaacg gcaaggcgct actggaagtg tctggcaacg tcactgacaa
     3841 aacactgcgc gaatttgccg aaacgggcgt ggactttatc tccgtcggtg cgctaactaa
     3901 acacgtgcag gcactcgacc tttcaatgcg ttttcgctga ttgctgacag atttatccgc
     3961 tccatcgcgg gcggataatc tcccctttcc caactttctt cgtaacgcct cgcaaatttc
     4021 ttgtctttcg ctgcaacgta ataaaaaact ccggaagctg ctttcccgtt tcgttttttg
     4081 cccctcgctt gtgctgatgg cgtttgccaa agtagcgcca accaaatcaa ggagcgaaac
     4141 aaatggacaa gcaacgcggt tttacactta tcgaactgat ggtggttatt ggcatcattg
     4201 ccattttaag cgccattggt attcccgctt atcaaaacta cctgcgcaaa gccgcactca
     4261 ccgacatgct acaaaccttt gtgccttacc gtaccgccgt agagttgtgc gcgctggaac
     4321 atggtggatt agatacctgc gacggtggca gcaatggcat tccctcgcct accaccaccc
     4381 gctatgtttc agccatgagt gtggcaaagg gcgtggtgtc gctgaccggg caagaaagtc
     4441 tcaatgggct aagcgtcgtc atgacaccgg gttgggataa cgcaaacggc gtcaccggct
     4501 ggacgcgcaa ctgcaatatt caaagtgaca gcgcattgca gcaagcctgc gaagatgtct
     4561 ttcgctttga tgacgccaac taaggagcgg caatgaatat tccacagctc accgccctgt
     4621 gtctgcgtta tcagggagtc ttgctggatg ccagcgaaga ggtggttcat gttgcggtgg
     4681 tcgatgcccc ttcgcatgag ctactggacg cattgcattt cgctaccacc aaacgtattg
     4741 agatcacctg ctggacgcgc caacaaatgg aaggtcacgc cagtcgcacc caacagacat
     4801 tgcccgtagc tattcatgag aagcatcagc ccaaagcaga gttgctgact cgaacgttac
     4861 aatctgcgct ggaacaacgc gcgtctgata ttcatatcga accagcggac aatgcctacc
     4921 gcatccgctt gcgtatcgac ggtgtattgc atcctttacc ggatgtttta ccggatgccg
     4981 gagtcgcatt aaccgccaga ttaaaagtgc tgggcaacct ggatattgcg gaacatcgcc
     5041 tgccgcagga cgggcaattc actgtcgaac tggcaggaaa cgccatctca tttcgtattg
     5101 cgaccttacc atgtcggggt ggtgaaaagg tggtattaag gttgttacag caggtgagtc
     5161 aggcactgga tgtcaacacg cttggaatgc agccgttaca actggcggac tttgctcatg
     5221 ccttgcaaca accacaggga ctggtgctgg taacaggccc tacaggcagc ggcaaaacgg
     5281 tcacgcttta tagtgccctg caaacgctga ataccgctga cattaatatt tgtagcgtcg
     5341 aaaatccggt tgagatcccc atagccggac taaaccagac gcaaatccat ccgcgtgccg
     5401 gactcacctt tcagggcgtt ttacgtgcgt tattgcgcca ggatcctgac gtcattatga
     5461 tcggagagat ccgcgatggc gaaacggcag aaattgccat taacgccgca caaacgggtc
     5521 atctggtatt gtccacccta cacaccaact ccacctgcga aacgctggta cgtttacagc
     5581 aaatgggagt cgcccgctgg atgctctcat cagcgcttac gctggtaata gcccagcgtc
     5641 tggtacgcaa actttgccca cattgtcgcc gacagcaagg ggagccaatc catattccag
     5701 acaatgtatg gccgtcgccg ctgccccgct ggcaagcacc cggttgtgta cattgctacc
     5761 acggttttta tggtcgcacg gccttatttg aagttctgcc cataacaccg gtcatacgtc
     5821 agcttatttc cgctaatacc gacgttgaat cgctggaaac gcacgcccga caggcgggta
     5881 tgcgaacgct ttttgaaaac ggctgtctgg ccgtggagca aggcttaacc acctttgaag
     5941 agttaatccg cgtattgggg atgccgcatg gcgagtaagc aactctggcg ctggcgtggc
     6001 ataaccggcg acggcaatgc gcaagatggg atgctatggg cagagagccg tgctttgctg
     6061 cttatggcac tacagcaaca gatggttacc ccactaagcc tgaagcgaat cgccatcaat
     6121 tctgcgcagt ggcgaggaga taaaagcgcg gaagtcattc atcaactggc gacgctactc
     6181 aaagccgggt taacgctttc tgaagggctg gcactgctgg cggaacagca tcccagtaag
     6241 caatggcaag cgttgctgca atcgctggcg cacgatcttg aacagggcgt tgccttttcc
     6301 aatgccttat ttccctggtc agaggtattt ccgccgctct atcaggcgat gatccgcacg
     6361 ggtgaactga ccggtaagct ggatgaatgc tgctttgaac tggcgcgtca gcaaaaagcc
     6421 cagcgtcagt tgaccgacaa agtgaaatca gcgttacgtt atcccattat cattttagcg
     6481 atggcaatca tggtggttgt ggcaatgctg cattttgtgc tgccggagtt tgctgctatt
     6541 tataagacct tcaacacccc actaccggta ctaacgcagg ggatcatgac gctggcagac
     6601 tttagtggcg aatggggctg gctgctggtg ttgttcggat ttctgctggc gatagccaat
     6661 aagttgctga tgcgccgccc gacctggctt atcgcgaggc agaaattgct gttacgcatc
     6721 ccgattatgg gttcactgat gcggggacaa aaactcacgc agatctttac gattctggcg
     6781 ctgactcaaa gtgcaggcat tactttttta cagggcgtag agagcgtcag agaaacaatg
     6841 cgctgcccgt actgggtgca acttctgaca caaatccagc acgatatcag taacggtcaa
     6901 cccatctggc tggcgctaaa aaatgccggt gagtttagcc cactctgttt gcaattagtg
     6961 agaacagaag aggcatccgg ctcgctggat ctcatgttag acaacctcgc ccatcatcat
     7021 cgggataaca caatggcgct ggcggataac ctcgcagcct tactggaacc ggcgttgctg
     7081 atcataacgg gaggaattat cggtacgctg gtggtggcaa tgtatctgcc aattttccat
     7141 ttaggcgatg cgatgagtgg aatgggataa cgctggcgtg ttgctccacg ccagcgttgg
     7201 gagattacag gttgttgaag atgcggtttt cttgttccag cacacggata aatgtggtgc
     7261 gcttggtcag ctctttcagg cgtgaagccc caacgtatgt acaagctgaa cgcaggccgc
     7321 ccaaaatatc acgcgcggta ttttcaaccg ggcctcgcag cggcagctta acggttttac
     7381 cttctgctgc gcgatattcc gcaacgccac caacgtgacg tttcatcgca gactcggagc
     7441 tcatgccgta gaacagcata aatttctcgc cgttctcctc aacgatgcga ccgccgctct
     7501 cttcgtggcc cgccagcatg ccgccaagca tgacgaaatc ggcaccgccg ccaaaggctt
     7561 tcgcgacatc gcccggcgtg gtgcagccac cgtcgctgac gatcattccg cccagaccgt
     7621 gcgcagcatc ggcacattcg atcaccgcag aaagttgcgg ataaccgacg cctgttttga
     7681 cgcgagttgt acaaacagaa cctgggccaa tgccaacttt aacgatatca gcaccggaga
     7741 ggataagctc ctcacacatt tcaccagtca ctacgttgcc agcacaaatg gttttggtcg
     7801 gccacgcttc acgcgctttc gcaacgaact gcacgaagtg ttcggaataa ccattcgcca
     7861 cgtcaataca aacgaagttt aatgccgggt tcaggtcgag aatctgttta gttttttcga
     7921 aatccgcatc agacgtgccg gtagaaacca tcacatgttt cagcacatca gcggaagaat
     7981 tgttgataaa cgcttgccac tcttcgaaag aatagtgttt atgcacagca gtcaaaatat
     8041 caaaagaagc cagcgcagag gccatagaaa atgtgcctac ggtgtccata tttgcggcga
     8101 taatcggcac gccggaccag ctctgacctg aatgtttgaa ggtgaattga cgttccagtt
     8161 caacatcgga acggctttta agagtggagc gtttagggcg gatgagaacg tctttaaaac
     8221 ctaacttcag atcttcttca atacgcatgt gcggattcct ggggttaatg gctataaggg
     8281 taagagcgca actccagtga cgctatcata cgcactaatc aatgcggcgc aagactgcga
     8341 aattgtcttt ttttacgcta caatcccata aatttacctg gtaaatagtg agcgaacgtt
     8401 tcatcaattt cgctttcgtg cgctcatttt taacgttttg ataatcatga tgaattcccg
     8461 ggaatataaa ttatgaggta tatagttgcc ttaacgggag gcattggcag tggcaagagt
     8521 accgttgcca atgcgtttgc tgatctcgga attaacgtca ttgatgccga tattattgcg
     8581 cgtcaggtgg ttgaaccagg tgcacctgcg ctacatgcca ttgctgatca ctttggcgct
     8641 aacatgattg ctgctgatgg aacattgcag cgccgggcct tgcgcgagcg gatcttcgcc
     8701 aacccggaag agaaaaactg gcttaacgcc ctgctgcatc cgctgattca gcaagagacg
     8761 caacaccaga tccagcaagc aacctccccc tatgtactgt gggttgtgcc attgctggta
     8821 gaaaactcac tgtataaaaa agcgaatcga gtgctggtgg tggatgtcag cccagaaacg
     8881 caacttaagc gcaccatgca gcgcgatgat gtaactcgcg agcatgtcga acaaatcctt
     8941 gctgctcagg caccgcgcga agctcgcctt gccgtggcag atgacgtcat tgataataac
     9001 ggcgcaccgg atgctatcgc atcggatgtt gcccgcctgc acgcacacta tttgcagctt
     9061 gcgtcgcagt ttgtctcaca ggaaaaaccg taatgcagac ccaggtcctt tttgaacatc
     9121 cactaaatga aaaaatgcgt acatggctgc gcattgagtt tttaattcaa caactcaccg
     9181 taaatttgcc cgtcgttgac tacgctggcg ctctgcattt cttccgcaat gtcagtgaat
     9241 tgctggatgt tttcgagcgc ggcgaagtcc gcactgagtt gttgaaagaa cttgaccggc
     9301 agcaacgtaa actccagacc tggattggcg tgcctggcgt ggaccagagc cgtattgaag
     9361 cattaattca gcagttaaaa gcggcgggga gcgtattaat ttccgcgccc cgtatcgggc
     9421 aatttctgcg tgaagatcgt ttgattgccc tggtgcgtca gcgactgagc atccccggcg
     9481 gctgttgtag ctttgattta cctacattgc acatttggct gcatctaccc caggcgcagc
     9541 gcgacagcca ggtagaaacc tggattgcca gcctgaaccc gctcacccag gcacttacca
     9601 tggtgctgga tttaattcgc cagtcggccc ccttccgtaa acaaaccagc ctgaatggtt
     9661 tttatcagga taacggtggc gatgccgact tgctgcgcct gaatctgtca ctcgattcac
     9721 agctttatcc gcaaatttcc ggtcataaga gccgttttgc cattcgtttt atgccgctgg
     9781 acagtgaaaa cggacaggta ccggaacgtc tggatttcga actggcctgt tgctaaggag
     9841 taaaaatgtc agaaactatt acggtgaatt gcccaacctg cgggaaaacg gtggtgtggg
     9901 gtgaaatcag cccgtttcgg ccattttgct ccaaacgttg tcagctgatc gacctcggag
     9961 aatgggctgc tgaagaaaaa cgaatcccca gcagtggcga tctttccgaa agcgatgact
    10021 ggagcgaaga gccaaagcag tgacatttta tctgatgcga cgctggcctg ataagacgcg
    10081 ggcagcgtcg catcaggcat cgtgtgcaaa tgtcggatgc ggcgggaacg ccttatccga
    10141 cctacaagac cttacatatc aacatgttaa aattgtagat actgataaga cgcgccagcg
    10201 tcgcatcggg catcttgtgc aaatgttgga tgcggcataa acgcctatcc gacatacaca
    10261 cgcttaagct tcgcaagtac cggttcattg gctggcggaa aatcatcggc attcagaccg
    10321 accagcgaca tccactcacc gggttgccct tctttacccc acggctcccc ttcccagctt
    10381 tcgaccagcc agaaccacag tgttatatgc ctgtccggga attcatattc cagtatttca
    10441 aatagcgaaa gatgttgtgg ggtaatcccg acttcttcct gaagttcacg caccaccgcc
    10501 tgttccggcg tttcacccat ttcaatttta ccgccgggaa actccagttt attcgccatg
    10561 tgcgcatctg ctgcgcgacg ggttataaag atttcattgt tctcgttgcg aataatacct
    10621 accgcaattt gcagcttttt cattgtctta aacctataaa aaaggcgcag aatcctgcgc
    10681 cttttacttc aactgttagc ttttattgca ggcggccatg gcactgctta tattttttac
    10741 cagaaccaca cgggcaagga tcgttacgtc ctactttgcg ttcaccggtt tgcgccgcca
    10801 gtgcagctgc ggcagcagag tcgtcatcct gatggctaag ctgctgcatt tgcgctaaac
    10861 gctcggcttc catacgacgc tgttgttcca gctcctcaac ctcttcaggc atacgtacct
    10921 gaactttgct cagcgtgctg ataacttcat attttaacga ctccagcatc gctgcaaaca
    10981 tggagaacga ttcacgtttg tattcctgct tcggatcttt ctgtgcatag ccacgcaggt
    11041 ggataccctg acgcagatag tccatcgcag ccaggtgctc tttccacaga gagtcaagcg
    11101 tttgcagcat gacgcctttc tcgaagtgac gcatcatctc agcaccaacc acttcttctt
    11161 tacgctgata cacttcgatg gactgcgcca gaatgcgctc acgcagcgtc tcttcatgca
    11221 gttctggttc tttatccagc cactcggcaa ttggcaaatc gaggtcgaaa tcgttcttca
    11281 gacgttcctg cagccccgga atatcccaca tttcttccag cgactgtggc ggaatgtagg
    11341 catcaatggt cgctttgaac acatcttcac gaatgctgtt gatggtttcg ctcacatcgc
    11401 tgacatccag cagttcgtta cgctgggagt aaatggcgcg acgctgatcg ttagccacgt
    11461 catcatattc cagcagttgc ttacgaatgt cgaagttacg gctttcaact ttacgctggg
    11521 cgttggcaat cgctttggtc acccacgggt gctcaatggc ttcgcctggc ttcataccca
    11581 gtttacgcat catgccggat actcggtcgg aagcaaaaat acgcatcagc gcatcttcca
    11641 tcgacaggta gaaacgggaa gaaccagcat ccccctgacg accagaacga ccgcgcagct
    11701 ggttatcgat acgacgggat tcgtgacgtt cagtaccgat gatatgcagg ccacctgctg
    11761 ccagtaccgc atcgtgacgt acctgccagt cggctttaat tttttcaatt tgctctgcag
    11821 tcggattttc cagcgcggca acttctgcct gccagctacc accgagcaca atatcggtac
    11881 cacgacccgc catgttggtc gcgatagtca ccgcagccgg gtaacctgcc tgagcaacaa
    11941 tcgccgcttc gttggcatgg aatttggcgt tcaggacgtt gtgcttaata ccggctttgg
    12001 tcagttcatt tgacaccagc tccgattttt cgatggagat tgtacccacc agcaccggct
    12061 ggcctttcgc agtgcgttct ttgatatctt caatgatcgc ctgaattttt tccgcttcag
    12121 tcatgtagac caggtccggc agatctttac gaatcattgg acggttggtc ggaacaacga
    12181 cagtatccag cttatagatg gagctgaatt cgaaagcttc ggtatcagca gtaccagtca
    12241 tccccgccag tttttcatac agacggaagt agttctggaa ggtgatcgaa gccagcgttt
    12301 ggttttcgtt ctggatctgc acaccttctt tcgcttccac agcctggtgc agaccatcgg
    12361 accagcgacg gccctgcatg gtacgaccgg tgtgttcgtc aacgatgata acttcaccat
    12421 ctttaacgat gtagtcgacg tcgcgggtaa acagcgcatg agcgcgcagc gccgccgtta
    12481 cgtggtgcat cagcatgatg ttggccggag agtacagaga ctcaccttca tccatgatgc
    12541 cttctttaac cagcagttct tcaatcagaa ccagaccacg ttcggtcagg ttcacctggc
    12601 gagatttttc atccaccgag aagtggcctt cgccctggaa ggtttcggag tcttcttttt
    12661 cctgacggat caggtgtgga ataattttat tcacgcgttt atacatttcc gagctgtctt
    12721 ctgccgggcc ggaaatgatc agcggtgtac gcgcttcatc gatcaggatg gagtccactt
    12781 cgtccaccag cgcatagtgc agtttacgct gtacacgttc ttcaggactg aacgccatgt
    12841 tgtcgcgcag gtagtcaaag ccgtattcgt tgttcgtacc gtaagtaatg tcagctgcgt
    12901 aagcttcacg ctttgccggt gctggcatgc ccggcaggtt gataccaact gtcaggccaa
    12961 gaaattcaaa cagcggacgg ttgttttcgg cgtcacgttg cgccaggtag tcgttgacgg
    13021 taactacgtg cacgccttta ccggttagtg cgttcaggta agcaggcagc gttgcggtca
    13081 gggttttacc ttcaccggta cgcatttcgg cgatgcagcg ttcgttaaga accataccgc
    13141 cgagtaactg aacgtcgaag tgacgcatac caaagacgcg cttacttgcc tcacgcacca
    13201 cggcgaaagc ttccgggatc agattttcca gcacttcgcc tttttccaga cgtgcacgaa
    13261 actctgcggt tttccctttc agttcttcgt cggagagttt ttccatctcc ggttccatgg
    13321 cattgatgat gttgaccact ttgcgcatcc ggcgcagggt gcgatcgtta cgactaccga
    13381 aaactttagt taataatttg attagcataa taaaatctca aacgccccgc gttgcggagt
    13441 taataaaatg aagtaacggt ttattgttgt tagctgaggc gttgagggcc agcacggatg
    13501 ccttgcgcct ggcttatcca gacgggcgtg ctgaattttg cttgcggggt aaaatgcgca
    13561 taatcaatgc gataaccctt ttcagacggc gtgccttcct gggtcaacag cgcgctgagc
    13621 gtatccagta atgcaagatg ttgcgcctga agaggcaaag attcttcagc aacgggcagc
    13681 gtttgcggtg ccattgcgaa ggaaagatga cggattaccg tgcgaatggc atgttgatgc
    13741 cagtaatcaa cggaatagtt cgaattcggg cgacgcgtgt tcgcttccag caaggccaat
    13801 tgaccaaagt taactttggc tgaaggctcg tggttgcggg ttgtcgcttt tgcgggcgcg
    13861 tttggttcgg cggcgttgct gagcgcaggc aaacctaaac tcgccgcgac catccctaat
    13921 aagagatgcg gccagaagta gcgtttacca aactgtcgcc agcgcgtcag tattccactc
    13981 acgttattgc catcccgatc gtattcaaac gtatttagcg cacaaatctt atcattaaag
    14041 cctgaagtcc acagcacgaa tgaagataag ttgtgcgtaa aagtgcttaa gaaaacagcg
    14101 ttcgcaccgt tttttatcgt tcttataaaa ttcaaaaaaa aacgactggt tcacctggcc
    14161 ggagagagtg ccagatttgc cagtcgaatt ttatacgaca gtataaatgt cgttatgcca
    14221 gtacggctga aggcgctttg aaggccaacg gcagttctgc gtcgtcctgg aaggtcacat
    14281 attcccaggc ttcctgtttc gccaggacag cctgcagcag tttgttattc agtgcatgac
    14341 cagatttata agcggtaaat gcaccaataa tattgtgacc acacatgaac aagtcaccga
    14401 tcgcatcgag cattttgtga cgcacaaatt cgtcttcaaa acgcaggccg tcttcgttca
    14461 gtacgcgata atcgtcaaca acgatggcac aatcgaagct gccgcccagg cacaaaccac
    14521 gggactgcag atattcgata tcacgcatga aaccgaacgt acgcgcacgg ctgatctggc
    14581 gcataaacgc atcagcggag aagttcatcg catagcgctg gttgctggaa tcaatagccg
    14641 gatggttaaa atcgatggtg aaatccagcg aaaaaccatt gtacggctta aattcagccc
    14701 acttatcgcc atcttcgaca cgaacagtct ctttgatgcg aacaaatttc ttggcgcagt
    14761 tcagctcgtc gataccagcg tcaagcagca ggtatacaaa cggagcggcg ctgccgtcca
    14821 tgatcgggat ttccggcgcg ttaacttcga taacaatgtt atcgatgccc aggcccgcga
    14881 gagcagcatt gaggtgctct acggttgaaa tccgtacatc atgctcgttg accagacacg
    14941 tacagagcat ggtatcacgc acagatttgg catcggccgg gaaatctacc ggtggattca
    15001 agtcggtgcg acgatagatg accccggtgt tggccggcgc agggcgtaac gtcagggtga
    15061 ctttcttgcc ggtatgtaaa ccgacacccg tcgcctgaac gatacgttta agtgtccttt
    15121 gtttgatcat cgtattatct cgccaaatta cctatccaac cgaagtgtac tatacattcg
    15181 gcgggccagt ttagcacaaa gagcctcgaa acccaaattc cagtcaattc ttaatcagct
    15241 tgcttacgca ggaatgctgg gatatccaga taatccggct ctttcgcagt ttgcggcgca
    15301 ttgtcattca cgactttagc aaccggcttc tgctcctggg tcagcggagc catcccgtgc
    15361 tgctggtagc gatccatcac tggctgctga acctgcttat tggtcaccag agtgatttca
    15421 ggacgtttgt ccatgccgat acctgtcgca acaacagtta cgcgcagctc gtcattcata
    15481 tccgggtcaa gagaagtacc gataaccaca gtcgcgttgt cggaagcaaa tgcacggatg
    15541 gtgttaccta ccgtttcgaa ctcatccaga cgcaggtcga agcccgctgt gatgtttacc
    15601 agtacgccgc gtgcgccaga caggtcgata tcttccagca gcggagaaga gatagccatt
    15661 tcagcagctt cttccgcacg atcttcaccg ctcgccacac cagaacccat cattgcgtag
    15721 cccatttcag acattacggt gcgtacgtct gcaaagtcca cgttcatcaa acccggacga
    15781 gtaatcagtt cggcgatacc ttgcacagcg cctttgagta cgtcgttcgc tgcgccaaac
    15841 gcatccagca gggagatacc gcggcccaga actttcagca gtttgtcgtt cgggatagtg
    15901 atcagagagt ccacatgctt ggacagttca gtgatcccct gctccgcgaa tgccatacgc
    15961 ttcttgcctt caaagttgaa gggcttagtg acgacagcaa cggtcaggat acccaaatct
    16021 tttgccactt cagcgacgac tggagctgcg cctgtaccgg taccaccacc catacccgca
    16081 gcaataaaga ccatgtctgc accttccagc gccgcacgca gtgcatcgcg atcctcatca
    16141 gccgcattgc ggccaacttc tggattagcg ccagctccca gtcctttggt gataccgcta
    16201 ccgatttgaa tcgtctgtcc aaccgctgtt ttacgcagcg cttgtgcatc ggtatttacc
    16261 gcgaagaatt caacaccttc aatgcgctcg cgcaccatgt gttcaacagc attaccgccg
    16321 ccgccgccga cgccgatgac tttaatcacc gcgtcattgg taagttccat tggttcaaac
    16381 atagtttctc tccgatttgt gcctgtcgcc tgagaccgta atcattgtcg gcctcataaa
    16441 aattaaaact cttttcgcag ccaactattg agtcgcttga tccacgagcc aactgatgct
    16501 gtaacacgtt tttctacttc agcttcaccg ttaagatgtg actctttccc gtaatgaagc
    16561 aatcccaccg ccgtcgaata atacggctcc tgagcataat ccgttaaacc ggtaatgttc
    16621 agcggcgcgc cgatacgcac ctgcgtatga aacacgcgct gagcacaggc tgcaagacct
    16681 tcaatctgcg ctgcgccacc ggttaataca atgcctgccg ccaggtgatg tttaacccct
    16741 tgttggcgaa gcttttcctg caactgcaat atctcttcgt tgaccaggtt gagcagctcg
    16801 gtatagcgcg gctcgatcac ctctgccagg gtctgacgtt gcagactccg cggcggacga
    16861 ccgcctacgc tcggcacttc cacgctctca tcttttccaa cgatggaacc cagcgcacaa
    16921 ccgtggcgaa ctttaatcgc ttcggcgtcg cttggcggcg tgccaaaggc gtaagcgata
    16981 tcactggtca cgacattgcc agcataagga attaccttag tgtggcgcaa tgccccaccg
    17041 gtataaacgg cgatatccat tgtaccacca ccgatatcga cgacgcagac acccagttca
    17101 cgttcatctt ccgtcaatac cgaataactt gatgccagtc cggcaaatat cagttggtca
    17161 actttcagcc cacaacgttc aaccgctttg acgatgtttt tcgccatatc gttgtgacat
    17221 gtgatcaggt gcacttttgc ctgcatccgc acgcccgaaa gtcctaccgg attcttgatc
    17281 ccttcctgat agtcaatcgc atactcttgc gggatcacat gcagcacacg gtgttcatcg
    17341 cgcacacgca ccgatttcgc ggtatggaca acgttttcca catcttcttg cgtcacttct
    17401 tcttcagaaa taggcaccat accaatttca ttctggcagc tgatgtgctt accagaaagc
    17461 gccagatata ccgaagagat ctgacaatct gccatcaatt ctgcctggtc aatggcgcgc
    17521 tgtacgcact tgaccacgga ttcgaggtcg ttcaccccgc ctttatccat accacgcgac
    17581 gggcagctgc ccacgccaat gatattgacc ataccgtcgg gcagaacttc ccctactaaa
    17641 gcggcaacct tcgcggtacc aatctccagt cctactacca gttttctgtc cgtcgcctta
    17701 atcattgttg ttctgcctgt gcctgatttt gttgctgagt agattcctct ggcggcaagg
    17761 gcgcccagcc tactgccgct ccagagtcat aacgcaaatc aacgtagcta atccgtttgc
    17821 catcggtttg cgcctgctgc tgtaaaaccg gataaagttc tacaaagcga gccaaacgtt
    17881 tcatcgtatc gccccggcca agattgagct taatatcgtt attcagcgtc aactgccagg
    17941 aacgccgcgc ggtcatcgcc gcttccttca gagtaaatct gtcctttgcc agcatctgcc
    18001 ccatttcgcg atagccctgc aacacttcat tggcgctgcc ttccgggcca tacagcattg
    18061 gaagcacctg cttgctggtg cgatctggcg gcacgctgaa agtgtttcct tccgcgtcta
    18121 ccatatgttg atcattccac cgcgcaatcg gcacatattc aaccagatga atcttcaatt
    18181 catcaggcca ctgctttctg acgctcacct gcttaatcca cggcaggcgt tgttctattt
    18241 gcgtctggat gatgttgaca tcctgggtca taaaggtacc tggctcaccc aatgccagga
    18301 tcgactgccg gatatcgtca ttacgcgtgt aatggcgttc accggtcaac accagctttg
    18361 agagcggcag gcgttgcgca tcttccatcc agcccaacac gacccagccg ctcaccaaca
    18421 ctgtcgttaa aacggtcagc aggaaaagga tccccgccag acgcgttcca ttattgcggc
    18481 gagaagaaac ctcttcttcg ctgtttcgcg tgttcagagc agcctgcgac atattagtcc
    18541 gccagttcca gaattcgtac taccaactgc gagaagctca tacctgcctg acgtgccgcc
    18601 atcggcacca ggctgtggct ggtcataccc ggtgaggtat tggcttccag cagataaaac
    18661 tgtccatcgc tgtccagcat aacgtcaata cgtccccatc ctttgcaacc taacgtcgtc
    18721 catgctttca gcactaatgc ctgcaaattg gcctcttgtg acgcttccag acctgcgggg
    18781 cagaaatact gtgtctcatc agagagatac ttcgcctcat aatcatagaa ggttccggac
    18841 ggttgaatac gtattgacgg taaaatttct tcaccgagta tcgcaaccgt gaactccggc
    18901 ccacttagcc atttttcaat caatacttct tcatcgtgct gaaatgccaa tcttaatgca
    18961 tcttgtagag cattttctgc tactactttt gacattccca cactggaacc ttcgcggctc
    19021 ggcttaacga taaccggcaa acccagagca gaaatttctg ctaactgctt atcactcagg
    19081 cctttttcaa actctgcgcg ggttaacgct acccacggag cgaccggtaa accggcacct
    19141 tgccacagaa gtttgctgcg tagtttatcc attgaaagcg cagatgccat cactccgctg
    19201 ccggtataag gcaagcccat cagctcgagc atgccctgca gcgtgccatc ttcaccgccg
    19261 cgaccgtgta gcgcgataaa cactttctga aagcccatcg acttcagttg cgtcacgtcg
    19321 acttctttcg ggtcgacagg attcgcgtca ataccgcctt cacgcagccc ggctaacacc
    19381 gctgcgccag aattcagaga aacttcccgc tcagcggagg tcccacccaa caggaccgcg
    19441 attttatcag tcatgttgtt cttcctccgg agtttgcggc ttcagtttga tttcagctaa
    19501 agaacgggca atttttccga tattaccagc cccctgaacg agaatcaggt cgttaccggt
    19561 taataccgga gccagcatct cggctacctg cgccgggtcc ggcaccagaa tgggatcaat
    19621 tttcccacgt ccacgaattg tgcgacacag cgaacggctg tccgctcccg gaattggcgc
    19681 ttcgccagcc ggatacactt ccagcatcaa cagggtatca acctgcgtca gcacattggc
    19741 gaaatcatca tacaggtcgc gcgtacgggt aaaacggtgc ggctgaaaca gcattaccag
    19801 gtttttatcc ggccagcctg cacgcgccgc tttaatggtg gcgtccactt ccgtcgggtg
    19861 gtggccgtag tcatcgacca gcattgccgt accgctttta ccattcacag gctccagcgg
    19921 gaattcaccg aggaaatcaa aacggcgacc agtcccctgg aagctctcca gcgcacgcaa
    19981 aatagcctcg tcgtcaattc cctcttccgt agcaaccgca accgcagctg cggcgttcag
    20041 cgcgttatga cggcctggcg cattcagggt gacgcgcatc ggctctttgt cctggcgcag
    20101 cagcgtaaag tgcccctgcg ggccaatctg ctgataatct tctacacgca cgtcggcatc
    20161 ttcgctgaag ccgtaagtcg tggtctgacg tcccacacgc ggtaataatt cgcggatcac
    20221 cggatcatca acacacatca ccgcacgacc gtaaaacggc aggttgtgca gaaaattaat
    20281 aaaggtctgt tttaaattct caaagtcgcc ctggtaggta tccatgtggt cggcttcgat
    20341 attggtgaca atcgccacca tcggttgcag atgcaggaac gatgcatcac tctcatctgc
    20401 ttcggcaatc aggtaccgac catgccccaa acgcgcatga acgcccgccg cttttaccag
    20461 cccaccgtta acgaaggttg ggtcgagtcc cgcttctgcg tagatgctgg aaaccatcgc
    20521 agtggttgtc gttttgccgt gcgttccggc aatggcgatg ccatgacgaa aacgcattaa
    20581 ctcagccagc atttcggcac gacggatcac cggaatacgc gcttcatgag ctgcgacaat
    20641 ttccgggtta tcggcagaaa tcgcgctgga aacaacgacc acgctggcat cacgtacgtt
    20701 ttccgggcga tggttgaaat aaatcgtcgc cccaagattc attaactgct gcgtgaccgg
    20761 atttggcgct aaatcggaac cactgatctg ataaccttca ttggccagaa cttcggcaat
    20821 accgcccata ccggcaccac cgatgccgac aaaatgtatg tgccgaacgc gacgcatttc
    20881 gggcacgatg gaacgcagtt ttgccaattg ttgtgtattc attctttacg ccattaactt
    20941 cttaaattca tacgatgcaa aaggcatcgc tacaattacg cccgggcagc ccggctcact
    21001 tcatttgcca ctcgctcggt ggcatccgga atggatgcgg cgcgggcgcg ttctgccatg
    21061 gttaataagg tttctcgcga ccacccggcc agggtgttgg cgacagcatc cacgctaagc
    21121 tgtggctgct cgataatttt ggctgcgccc gctttttcca gcggtagcgc attccagtat
    21181 tgctggcggt ctttatgttg aaacggcaca aacaacgccg gtagtcctgc cgcggcgatt
    21241 tcactcaccg ttaacgcacc ggagcggcaa acgacgacat ccgcccacgc atacgccgcc
    21301 gccatatcat caataaattc cgtcacttta tgctgcggtt gccccgcttc ggcatacgcc
    21361 tgttcaacgg attgttgcga acctttaccg ctctgatgcc agatagtgac tgaatcaccc
    21421 agtttcgcag caacctgcgg cattgtctgg ttaagaatgc gtgcgccctg ggaaccaccc
    21481 actaccaaca cacgaaccgg accttcacgt ccagccaaac gttgctgcgg caacggcagc
    21541 gccagcacat cggtacgcac cgggttaccc accacttccg cattagggaa agcgcctgga
    21601 aacgcctgca tcactttggt ggcaatcttc gccagccatt tattggttaa tcccgcaata
    21661 ccgttttgtt catgaagcac aaccggaata cctaacgacc acgcggccag accacctgga
    21721 cctgatacgt agccgcccat accgagcacc acgtcaggtt tgtacgcttt cataatcgcc
    21781 cgcgcctgac gccaggcgtt gaagatacgc agcggggcag ctatcagtgc ttttatacct
    21841 tttccacgca gaccagagat acgaatgaaa tcaatttcga tgccatgttt tggcactaag
    21901 tccgcttcca tacggtcggc agtccccagc cagcgaactt gccaaccctg agccattaga
    21961 tggtgcgcaa ccgccagtcc cgggaataca tgtccaccgg ttccgcctgc catcaccatt
    22021 aatcgcttcc cttgaccact catcgtgaac ctcgtacaaa cgcctgcgct ttctccagac
    22081 gcgtttcata atcaatacgc aacagcatca tgatggctgt cgacataatc agtaagctcg
    22141 aaccaccgta actgatcagc ggcaatgtca gacctttggt cggtaacatc cccgccgccg
    22201 cgcctacgtt aaccagcgcc tggaaactaa accagatacc aatagaacag gcgagaaaac
    22261 cggaaaaacg gtggtcaatt tctaatgctt tgcggccaat cgacatcgcg cgaaaagcga
    22321 cgaagaatac cattaaaagt gccagcacca caccgacata ccccagttct tcgccgataa
    22381 tggcgaaaat aaagtcagtg tgcgcttccg gcagatactc cagtttttgt accgagttac
    22441 ctaaaccttg tccccacagt tcaccgcgac caaacgccat caacgattgc gttaactgat
    22501 agccgctgcc aaagggatct tcccacggat tccagaatgc ggtaacacgg cggatacggt
    22561 acggttcggc gaggatcagc aacacaaccg ctgaaatgcc cataccgata atggcaatga
    22621 actgccacaa tttcgctccc gccaggaaca acatcgccag cgtagtcaca aacaacacca
    22681 ccaccgtacc aaggtctggc tgtgccagca gtaacactgc caacaccaga attacgccca
    22741 tcggtttcag gaagccgcgc aggttattac gtacttcgtc gcctttacgc accagatagt
    22801 tggcgatata gcaaaacagc gacagttttg tcagctccgc aggctggata cgcagcaaac
    22861 cgagatcgat ccaacgcgat gcccctttaa ccgagctacc cactaccaga acgatcatca
    22921 gcaggatgat agatccgagc agcatcgtgg cactgtagcg ttgccagaac tccatcggca
    22981 gacgcagcgt aatgatcgcc agaatgaatg ccaggatcag atagacgcca tcacgcttcg
    23041 cgaagaagaa cggatcgttg gttaagcgtt gccctatggg cattgacgcc gaggtcacca
    23101 tgataaagcc aatcgccgcg aggccgaagg tcagccacag taaggtgcga tcgtacatga
    23161 tcaggctgtc ggtatctttt tcccgcgagc ccatcaccca gccctttagc gccgtggaga
    23221 tccagaccag aatactgaat cctggcaggc gcggcatttt caggcgaggg agagataaac
    23281 gcatcaacct aactccttcg ccagacgggc aaactcattg cctcgttgtt caaagttctt
    23341 gaattgatca aggctggcac aggccgggga gagcagaacc atatcgcccg gctgaacacg
    23401 cggagccagc aagcgcatcg cctgttccat ggtttcggtt tgttctgcca cttccgggcg
    23461 tagcgccgcc agctgcgcgc cgtcccgacc gaaacaatac agacgtacat tatcgccatt
    23521 caggtaacgc gccagtgggc taaagtccgc cgatttacca tcgccaccca gcaacaaatg
    23581 cagcgtgccg tctacatgca ggccattcag cgccgcttcc gtactgccga cgttggtcgc
    23641 ttttgaatcg ttaacccagc gcacaccgtt atgctccagc acaacttcaa agcgatgcgg
    23701 cagaccagtg aatgtggtta acgctttcag gctgctggca cgcggtaacc ctgcggcatc
    23761 tgccagcgcc agcgccgcca gcgcattggt gtagttatgc tgcccggaaa gtttcatttc
    23821 tttcacgttc agcactttct cgcctttcac ccgcagccag gtttcaccct gctgatgatt
    23881 caggtgatag tcgcccatgt tgacgccaaa gctgacgcag cgttcatccg caccgcgaat
    23941 cggcattgtt aaggcatcat cagcattaac cacgcaaatt tttgcgtttt cgtaaatgcg
    24001 cagttttgct gcacgatact gttgtaaacc aaacggatag cgatccatat gatcttcagt
    24061 cacgttcaga atggtcgctg ctaccgcctg taaactggag gtggtttcaa gctggaagct
    24121 cgacagctcc agcacgtaca gttcgcactc atcatccagc agcatcaacg caggcaggcc
    24181 aatattgcca cccacaccaa cgttaacccc cgccgctttc gccatttcac ccactagcgt
    24241 ggtgaccgtg cttttgccgt tagaaccggt gaccgccaca atcggtgctt gtgcttcacg
    24301 acagaacagc tcgatatcgc caacgatttc gattccggca tcagcggcag cgcttaagga
    24361 tggatgcgcc agtgcaatac cgggactggc gacaatcaga tcggccgcca tcagccattc
    24421 atcattcaga ccgcccgtgt ggcgttctac agcttcgggt aatttatcca ggccaggcgg
    24481 tgtcatacgc gtatccataa cgcgcggcgt cacaccgcga gcgaggaaaa agtccacgca
    24541 agaaagcccg gtgaggccca ggccgataat gacgacattt ttaccctggt aatcagccat
    24601 gattaacgta ccttcagcgt tgccagacca atcaggacca gcatcagcga aataatccag
    24661 aaacgtacaa tgacgcgcgg ttccggccag cctttcagtt catagtggtg atgaatcggt
    24721 gccatgcgga aaatacgttg tccgcgcagt ttaaaggagc cgacctgcag gatgacggaa
    24781 agtgtttcta ccacgaacac gccccccata atcaccagca ggaattcctg acgcagcagt
    24841 acggcgataa tacctaacgc accacctaac gccagcgaac cgacatcgcc cataaagacc
    24901 tgcgccggat aggtgttaaa ccacaggaag cccagccctg ccccgactat cgcggtacag
    24961 acaatgacca gttccccggc gtgtcgtaga tacggtatgt gtaaatagct ggcaaagttc
    25021 atgttgccgg tcgcccacgc caccagcgca aaaccaccgg cgacaaatac ggtcggcata
    25081 attgccagac cgtcgagacc atcagtcagg tttaccgcgt tgcctgtacc cacaatgacg
    25141 aagtaagcca gcagaatgta gaacagcccc agttgcggca tcacatcttt aaaaaatggg
    25201 accaccagct gcgttgcggg cgtgtctttg ccaacaaggt acagcgcgaa ggcgacaccc
    25261 agcgcaatga ccgacatcca gaaatacttc caacgagcga tcaacccttt ggtgtcttta
    25321 cgcactactt tgcgataatc atcaacaaag ccaataacac cgtaacctac cagcactacc
    25381 aacacgcacc agacgtacgg attggacggg taagcccaca gcagtacgga gatcacaatc
    25441 gctgtcagga tcataatccc gcccatggtc ggcgtaccgc gcttgctgaa gtgtgattca
    25501 ggaccgtcgt tacgcaccac ctgaccaaag gaaagttttt gcaaatgagc aatcatgcgc
    25561 gggcccatcc acaatgagat gaacagcgca gtcagcaggc tgacgatggc gcgaaacgtc
    25621 agataggaaa agacgttaaa gccggaataa tatttgacca aatgttcggc cagccaaact
    25681 aacatgtccc attctcctgt aaagcgcgta ctacctcttc catggcagca ctacgtgaac
    25741 ccttaactaa aatcgtaatt acctgttgct cagcaatcag tgatttaaga cgcgcgataa
    25801 gcgcagtttt atcggaaaaa tgttcgccaa caccgctggc agtgctgata gcatggcttt
    25861 gtttgcccac gcttaacacg cggtcaatac cagctgcttt tgccgcttcg cccacctgta
    25921 tatggcaggc ttcgctttca gcgcccagtt ccgccatatc gcccaccacc agcacgcggt
    25981 agcccggcat ttcagccagt acctggactg ctgcggtcat tgaaccgaca ttggcgttgt
    26041 aggagtcgtc gagcagcaac tggttttccg ccagttggat ggggaacaga cggcctggaa
    26101 cagctttcag atttgccagc cccgctttga tagcatcaag cgttgcgccc acggacatgg
    26161 agagcgcagc ggctgccagc gcattcgcaa tattgtgacg ccccggcaac ggcagcagaa
    26221 catccacgct accggttggg gtttgcaggg taaattccgt accgtgcgaa gtcacatgga
    26281 tgttggtggc ggtgaaatcg ctgttggcgg cattaggtga gaaacgccac actttgcgtg
    26341 agccaattac gctctgccag ttcagccagt cgttgttgtc ggcgttcata atggcgatac
    26401 cgttttccgg caggccgcta aagatttcac ctttcgcttt cgcgacaccc gcaagcgagc
    26461 caaaaccttc cagatgcgcc gctgccaggt tgttgaccag cgccgcttcc gggcgagtca
    26521 gactcacagt ccaggcaatt tcgccctgat ggttcgcgcc aagttcaatt actgcgtaat
    26581 cgtactctgg cgttaagcgc aacagcgtca tcggtacacc gatgtcgttg ttgagattgc
    26641 ctgccgtata aagcgtgttg ccacactggc ttaaaatcgc tgccgtcatc tctttaacgg
    26701 aggttttgcc ggaggaaccg gtcagagcaa ccacgcgcgc cggaacttgc tggcgaaccc
    26761 atgcagccag ttcaccaaac gccagacgag tatccttgac gattacctgc ggcagatcga
    26821 tatccagcgg acggttaacc agtagtgcac ccgcaccgcc agctttcgcc tggtcagcaa
    26881 aatcatgggc atcgaaacgt tcgcctttca gggcaacaaa caggcaaccc ggcgtcagtt
    26941 ttcgtgtgtc tgtggttaca gcatcaaggg tgatatctgc cccttttagt tcaccgttga
    27001 gaatatcggt aagttggcta agggttacgc taatcatgca atcaccccca gcagacgcgc
    27061 caccgtgacg cgatcggagt agtccagacg ctgattgcca acaatctggt aatcctcatg
    27121 acctttgccc gcgaccagta ccacatcatt ctctttagcc tgcataacgg cgcaagtcac
    27181 cgcttcagca cggccttcca tcactttggc atgtccggca tctaacattc ccgccagaat
    27241 atcgttgatg atggcacgcg gttcttcggt acgcgggtta tcgtccgtca ccaccgccac
    27301 gtcagcgaac tcttcagcaa ttgcgcccat cagtggacgt ttgcctttat cacgatcgcc
    27361 accacagcca aagacgcacc acagcttgcc cgcacagtgc aggcgcgccg cctgtaaggc
    27421 tttttccagt gcatccggtg tatgcgcgta atccaccact accgtcggtt tgcctggcgc
    27481 agtgaacact tccatacgtc cacaaaccgg ctgcaggcgt gcggcagttt tcagcaggtc
    27541 agccaacgga tagcccagcg ccagcagcgt cgccagcgcc agcagcaagt tgctgacgtt
    27601 aaaagcgccc atcagatggc tttcaatttc gccatcgccc cagcttgagc taaagcgaat
    27661 cgtcgcaccg ctgtcgtgat agttcacttc tgtcgctttc aaccagcgtc cgtgacagtt
    27721 cggattgata tgatcttcca ttgataccgc aaccgcgtcc ggcagttttg ccagccagcg
    27781 gcggcccact tcatcgtcgg cgttaataat cgcctgaccg caatgatgct cagaataaag
    27841 cagccatttc gcggcttcgt agtgttccat atcaccatga taatcaaggt gatcgcggct
    27901 taagttggta aagaccgacg ccgcaaattt caatgccgcc acacggtgct gtaccagtcc
    27961 gtgggaggaa acttccattg cgcaaaaagt cgcgccctga tccaccagcc ccgccagctc
    28021 atgctgaaca tcgactgccg aaccggttgt attttctgtc gggatcactt tccccagcag
    28081 gccgttacca acggtgccca ttaccgcgct ggtttcgcca agcagttggc tccactgcgc
    28141 cagcagctgg gtcgtcgtgg ttttgccgtt ggtgcccgtt acgcccacga gacgtaaatt
    28201 gtcagagggt tcatggtaaa agcggcccgc cagtgcagat aaacgctcgt tgagctggct
    28261 gagatagatg accggtacgc cgtgcatttc acggatttca ccgtcggtcg cctcatcttt
    28321 cgcctctgca ataatggcag ccacaccttg cgctatcgcc tgcgggatat atcgacgccc
    28381 gtccgcctga tgacctacta cagctacaaa gagatcgccc gccgcagcca cacggctgtc
    28441 gagtgtcatc tctcgcagtg ctcgcgaagg tgcgtctggc acccacggag caagaaggtc
    28501 gcgcaaatta cgatctgcca cctgtcccct cgccttgatt aatcacaaat tcatttttat
    28561 cgcccgttgt cagcgcatcc ggttcgatgt tcatggtacg caatacgccg cccatgatgg
    28621 caccaaagac cggcgcggaa acggcgccgc cgtagtattt acccgcctgc ggatcgttga
    28681 taacaacaac cagcgcgaag cgcggctgac tcgcaggcgc aacgcctgcg gtataagcaa
    28741 tatatttatt gatgtagcga ccgtccggcc cgaccttttt cgcggtaccg gttttaatgg
    28801 cgatacgata gcctttaatc gccgccttca cgccgccgcc gcccggtagc gccacgcttt
    28861 ccatcatatg caccacggtg cgaacaatgg attccgggaa gactcgttca ccgggaaccg
    28921 gggggtcaac tttggtaatc gacagtgggc gataaatgcc gtagctgccg atagttgcgt
    28981 agactcgcgc taactgtaac ggtgttacca ttagcccgta gccgaaagag aaggtggccc
    29041 tctctatgtc agaccaccgt tgtttttgag gatataagcc actgcgttct ccgaccaacc
    29101 ccaaattggt cgcttttccc agtccaaaac gtgagtaagt atctactaac gctgaggacg
    29161 gcatcgctaa cgccagcttg gaaacaccga cgttactcga cttctgtaat accccggtca
    29221 gggttaattc gctgtagcgt gccacgtctt tgatttcgtg gccgttaatt cgataaggaa
    29281 cggtattcag taccgagttt tcccgcacta cgccacgttg caacgcggtc atcaccacca
    29341 tcggtttaac cgttgaaccc ggttcaaaca cgtcagtgat ggtacggtta cgcatcgcct
    29401 ctttcggcgt gccactcaga ttgtttgggt tgtatgacgg gctgttagcc atcgccagca
    29461 cttcaccggt gttgacatcc accagcacgg cgctaccaga ttcagctttg ttaaaggcca
    29521 ccgcgttgtt cagttcgcga taaaccagcg cctgcaggcg ttcatcaata ctcagcgcca
    29581 ggttgtgcgc cgcctgactg tcggtggaag agatatcttc aattacgcga ccatagcggt
    29641 ctttacgcac aatgcgctca cccggctgcc cggtaagcca tttatcgaaa ctcttctcga
    29701 cgccctcaat cccctgacta tcgacgttag taaagccgat gaggtgagcg gtcacttcgc
    29761 cggatggata ataacggcga gattcttcac gcagatgaat ccccggcagt ttcagttttt
    29821 tgatgtactc tgccatgtca gggtttacct gacgcgccag ataaataaag cgccctttcg
    29881 ggttggcgtt aatgcgggct gaaagctgat ccagcggaat attgagtgcg ttagccagcg
    29941 ccttccagcg atcaccgacg ctgataccgc cagcgtcatg cacttctttc gggtcagccc
    30001 aaatcgcttt taccggcacg ctcaccgcta acgggcgacc agaacggtca gtaatcatgc
    30061 cgcgggaggt ggaaacttgc tgaacgcgaa gagaacgcat gtcgccctct tttaccagca
    30121 tatccgggga gataacttgt aaccacgcta cgcgtccgag cagaaaagcc agcgccagga
    30181 gaatacagcc gcataacaac gcaaaacgcc aactgataaa gttggcatgt tcttcctgac
    30241 gttttggttt ctgcgttttc gccgctgctt tcatgcgtcg cgtttatcct tatttttgca
    30301 ctacgatatt ttcttgtgac ggatcaacat gctgcatttg cagcttttcc gtggcgatcc
    30361 tttccacccg gctatggtcg ccgagcgcat tctcttcaag gatcaggttg cgccattcaa
    30421 tgtctaaagc atctcgctcc agcaccagtt gttcgcgctg tgcggtcagt aaacgggtat
    30481 ggtgcgccgt ggttaccacg gtcaccgccg tcaaaataat gcaaatgaac aggcagagtg
    30541 gcagcttccc aaatcgcaaa agatcgtcac cgataacacc aggcaatgca tggcgctcgt
    30601 ggcttcccat cgatccttta actttgctta gagcttctgt cactctgctg atcatgcgtt
    30661 cgtcctctct gcaatacgca gaactgaact acgggcacga gggttctcag ccacctcttc
    30721 ttcacccggc attaacttgc ctagtgctcg cagctgacgg ccacccagtt ttttgagctg
    30781 ctcttcagtc atcggtaacc ctgccggaac ttgcggaccg cggctgtttt cacgcataaa
    30841 acgtttcaca atacggtctt ccagcgagtg gaagctgatg atcgaaagcc gcccacccgg
    30901 ggccagcacg ttgagcgagc tttttagcgc ctgctctatc tcctccagtt cactgtttac
    30961 ccaaatgcgc accgcctgga aggtacgggt cgcgggatgt ttaaacttat ctttcaccgg
    31021 cgttgcagca gccacgactt ccgccagttc tttggtgcgg gtcatcggct gttcgcggtt
    31081 acgctcgaca atggcgcggg caatgcgttt ggcaaaacgc tcttcaccat aggttttcaa
    31141 tacccaggcg atatcggctt cttctgcggt ttgtagccat tcagcggctg actgcccacg
    31201 ggttgggtcc atacgcatgt ccagcggacc atcgcgcata aaggaaaagc cgcgttcagc
    31261 atcatcaagt tgcggtgaag agacgccaag atcgaggaga atgccgtcga tcttgccgat
    31321 aagatcgcgc tcggcaacgt attcgcccag cgcggagaaa ggtccgtgga tgatggagaa
    31381 gcgcggatca tcaatagtct tcgcaacggc gatagcctgc gggtcgcgat cgatcgccag
    31441 caaacgcccc tcttcgccaa gctgcgagag gatcagacgt gagtgaccac cgcgaccaaa
    31501 agtcccatca atgtagatgc catcaggacg gatattgagg ccattaacgg cttcatccag
    31561 cagcaccgta gtatgtttat agttttccat cattttatag agacaagtcc tgcagtcgct
    31621 ccgataagtc tccagtagcc aactgctctg cgtcgatatc ttccttgacc tgttgatgcc
    31681 aggttgtttc atcccacagc tcaaacttgt tgaactgtcc aaccagcatc acttcttttg
    31741 tcagcccggc atgttgccgc agtactggcg cgattaacaa tcgacctgcg ccatccatct
    31801 gacattcgct ggcatgacct aacagtaggc gctgcacacg gcgctcaacc gggttcatgc
    31861 tcgacagacg cgataatttt tgctcgataa tttcccattc aggcaggggg taaagcagca
    31921 ggcacgggtg atgaatgtca atggtgcaaa ccatttgacc ggcagcgttc tcaagcagct
    31981 gttcccgata acgggtaggc actgataagc gccctttgct gtcgagattg actaacgttg
    32041 ctccccggaa catgccagcc tcacccctta ttcccacttt gccccacaat ttcccactga
    32101 aaggagttta cggagcggag gaaaagcttg tcaagccagt cacaaggcat accgaggtaa
    32161 aaaacccgat gtttacggct aatatcagcg ttgtttattt tacgtccaac gtgcgaagca
    32221 aattaacgtt atgaatagct gtaagaaaaa atccctcatc gtcatcaccg ttttaaaatg
    32281 actcactatt ttccacagca aatataaagt gtcagtttgc gacgcgagcg gcattttagg
    32341 acatatcttc cccggttaac agtccctgtt gcgtctgtgt ggcgcttgat gccagcgggt
    32401 ttgcgcaaaa gcaaagaaaa gtgtttgtta aatcggcaat tcatctctgc atgtaacaaa
    32461 ataatgcaaa aacgcgctat cgatatcact taattattgt gaatttcagc tggtgaggta
    32521 attaacgggc aggtttaaaa gcatttatca ataatagcca ggaatagtct tatttacttt
    32581 aaggatattg atgtccattc ccgtactcaa cgcgccagag agaaattcac ctggcgcgta
    32641 tttttgttcg cggcttagct acggctgagc acgccgcggc gatagagatt acgtttaatg
    32701 cgcgttaaac caggttttgg cttacgcggt tcgtccaggc ttgccaggac aatctccagc
    32761 acacgctctg cgacatcgcg gtgacgttga gccactgcca gcaccgggca ctgtaagaag
    32821 tcgagcagtt cgttatcgcc aaaggtggca attgccaggt cagaaggcag tttgccgtca
    32881 cgacgtagcg tgacatccat caccccttgc aacaacgcaa acgacgttgt gaacagtgcc
    32941 tgcggcatcg gatgcgtttc cagccatttt tcgaataact gggcagccgc ctcccgctca
    33001 tagctgttgg cataaaggaa atgcacttcg cgaggatcat ctttccaggc agtacggaaa
    33061 ccttgttcac gcaggaagct gacagaaagc tccggcagcg cgccaagata aagtaccgtc
    33121 tcggcgggaa acttacgtaa ctcttccgcc agcatttcgg catcatcctg atcggcacca
    33181 accacgctgg tgaagtgttc acgatcgagg gcgcggtcca gcgcgacaat cgggaacggg
    33241 tcgttagccc agcgttgata aaaaggatgc tcaggtggca acgacgtcga aacaataatg
    33301 gcatcaacct gacgctgtaa aagatgctcg atacaacgca tttcgttgtc gggctgatct
    33361 tccgagcagg caatcagcag ttgataaccc ctttgccgcg cctggcgttc aagatagtta
    33421 gcgatgcggg tatagctggt gttctccaga tcggggatca caagaccaat agaacgtgtg
    33481 cgtccagcac gaagcccagc tgccacggcg ttcgggtggt aattgtgctc acgcaccaca
    33541 gccatgactt tttcaacggt tttgtcgctc acacggtatt gcttcgcttt gccgttaata
    33601 acatagcttg cagtggtccg cgacactccc gccagccgag cgatttcatc cagtttcaca
    33661 attgcccctt gcgtaaaatg taaaaaccat agccattgta ctggtatggg ttaaattcat
    33721 taacatctaa gcgcagaaaa ttaactgcgg caactgcttt tattccaggt tcccactgat
    33781 ttcgcaaaaa aagcccaacg tgacatgttg ggctgcaaat tgcgcattga gatcattctc
    33841 aacgcattat tttatcgccg cgcgaaagtc cgactacacc agagcgagca acttccacaa
    33901 ttttcgccac atcgcgaatc gatgctaaaa atgcatcaag cttatcgctg gtgcctgcta
    33961 attgaacggt ataaagcgag ggtgtgacat cgataatttg cccacggaat atttccgtat
    34021 tacgtttcac ttcatcacgc ccgtaaccgc tggcctgaat tttcaccagc atgatttccc
    34081 gctcaacgtg cgcgccctgc cccaactcgc tcacgcgcaa gacatcgacc agcttgtgta
    34141 attgcttttc gatttgctca agcacttttt catcgcccac ggtctggatg gtcatacgcg
    34201 ataatgttgg atcgtcggtt ggcgcaacgg tcaggctttc aatgttgtag ccacgctggg
    34261 aaaaaaggcc aatcacgcgg gataacgcgc ctgattcatt ttcgagtaag actgataata
    34321 tccggcgcat aatcaggttc tctccgtttt gcttaaccac atttcatcca ttccgccccc
    34381 gcgaatctgc atcgggtaga cgtgctcact gccatcgacg gtgacatcaa caaacaccag
    34441 gcgattattg cgcacctgtt ccagcgcctc gctaagtttg ctttccagtt cttgcggatg
    34501 agagatctgg atcccgacgt gaccataggc ttccgccaga cgaacgaaat cgggtagtga
    34561 ctgcatataa gattgtgaat gacggccgga atagatcatg tcctgccact gtttcaccat
    34621 ccccagatag cggttattga gattcaccac cagtaccggc aactcgtatt gcaacgctgt
    34681 agacagttcc tggatgttca tctgaatact gccgtcgcca gtgacgcaaa ccacggtttc
    34741 ttctggcaac gccattttga cgcccagtgc cgcaggtaaa ccaaaaccca tcgtgccgag
    34801 gccgccagag ttaatccagc gacgcggttt gtcgaatgga taataaagcg cagcaaacat
    34861 ctggtgctgc ccgacatcgg acgtcacgta agcgtcccca ttagtcaacc gccaaagagt
    34921 ctcgatcacc gcctgcggtt taatcttttc actgtgagtg tcatatttca ggcactgacg
    34981 agcgcgccac tgttcaatct gctgccacca gtcgcggatc tcatccagcg gttgatggac
    35041 ggattcttgc gacaggagtt caagcatttg ttcgaggacc tggcgagcat cccccacaat
    35101 cgggatatca gcagtcacgg ttttagaaat ggaagtagga tcaatatcga tatgcagaac
    35161 cgtggcattt gggcagtact tcgccagatt gttcgtcgtt cggtcatcaa aacgtacccc
    35221 gacggcaaaa atcacatccg cgttatgcat cgtcatattg gcttcgtagg taccgtgcat
    35281 tcccaacatg cccagtgcct gacgatgcgt tgccggaaac gcccccagcc ccattaatga
    35341 ggaaacaacg ggcagattca acgcctccac cgtttctttt agctgctgat gacagcccgc
    35401 cgtgattgcc ccaccgccta catagacaac cggttttttt gccgccacca gcgtttgcag
    35461 agcgcgctta atttgccctt tatgcccgga ggtcgtggga ttgtaagaac gcatactgac
    35521 cgactccggc cagacatagg gtaatttgtt cgccggattt agaatatctt tcggtaaatc
    35581 aacgaccacg ggtccaggac gaccacttgc cgccagccag aaagcctttt tcagcacctg
    35641 cggaatgtct tccgtttgct taaccagaaa actgtgttta accaccgggc gagaaatccc
    35701 caccatgtcg cactcctgaa aggcatcgta gcctatcaac gaggtcgcta cctgcccgga
    35761 aaggacaact aagggaatgg aatccatata agcggtggca atgccagtaa tcgcattggt
    35821 cgcccctgga cccgacgtta ccagcacgac gccgacttcc ccggtcgcgc gcgccagacc
    35881 atcggccata tgcaccgccg cctgctcatg acgaactaaa acatgatcga taccacccac
    35941 ggtatgcaat gcatcgtaaa tatcaaggac cgcgcctccg ggataaccga atacttgttt
    36001 aacgccctga tcgataagcg atcggacgac catctcggct ccagacaaca tctccatggc
    36061 ctgcctcact gtttgacgga aaaaatgtgt aaaacacatt aaccgtccga tcttgcttaa
    36121 gcaattggca gcctgagaca taaacacggg gtaataagaa taaacaggag gaaaggtgaa
    36181 aagaaacgat aaaatcctcc attacagagg attaaattga taa
//
LOCUS       DAZFLD010000015        35561 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.015, whole genome shotgun sequence.
ACCESSION   DAZFLD010000015 DAZFLD010000000
VERSION     DAZFLD010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 35561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35561
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.015"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..219)
                     /gene="yeiS"
                     /locus_tag="ACHKBL_000714"
     CDS             complement(<1..219)
                     /gene="yeiS"
                     /locus_tag="ACHKBL_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2542 family protein"
                     /protein_id="HFO5817629.1"
                     /translation="MDVQQFFVVAVFFLIPVFCFREAWKGWRAGAIDKRVKNAPEPVY
                     VWRAKNPGLFFAYMVAYIGFGILSIGMIV"
     gene            complement(222..941)
                     /gene="sanA"
                     /locus_tag="ACHKBL_000715"
     CDS             complement(222..941)
                     /gene="sanA"
                     /locus_tag="ACHKBL_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane permeability protein SanA"
                     /protein_id="HFO5817630.1"
                     /translation="MLKRVFLSLLVLIGLLLLTVLGLDRWMSWKTAPYIYDELQDLPY
                     RQVGVVLGTAKYYRTGVINQYYRYRIQGAINAYNSGKVNYLLLSGDNALQSYNEPMTM
                     RKDLIAAGVDPSDIVLDYAGFRTLDSIVRTRKVFDTNDFIIITQRFHCERALFIALHM
                     GIQAQCYAVPSPKDMLSVRIREFAARFGALADLYIFKREPRFLGPLVPIPAMHQVPED
                     AQGYPAVTPEQLLELQKKQGK"
     gene            complement(1091..1975)
                     /gene="cdd"
                     /locus_tag="ACHKBL_000716"
     CDS             complement(1091..1975)
                     /gene="cdd"
                     /locus_tag="ACHKBL_000716"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HFO5817631.1"
                     /translation="MHPRFQTAFAQLADNLQSALEPILADKYFPALLTGEQVSSLKSA
                     TGLDEDALAFALLPLAAACARTPLSNFNVGAIARGVSGTWYFGANMEFIGATMQQTVH
                     AEQSAISHAWLSGEKALVAITVNYTPCGHCRQFMNELNSGLDLRIHLPGREAHALRDY
                     LPDAFGPKDLEIKTLLMDEQDHGYALTGDALSQAAIAAANRSHMPYSKSPSGVALECK
                     DGRIFSGSYAENAAFNPTLPPLQGALILLNLKGYDYPDIQRAVLAEKADAPLIQWDAT
                     SATLKALGCHNIDRVLLA"
     gene            complement(2105..2800)
                     /gene="yohK"
                     /locus_tag="ACHKBL_000717"
     CDS             complement(2105..2800)
                     /gene="yohK"
                     /locus_tag="ACHKBL_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidB/LrgB family autolysis modulator"
                     /protein_id="HFO5817632.1"
                     /translation="MMANIWWSLPLTLIVFFAARKLAARYKFPLLNPLLVAMVVIIPF
                     LMLTGISYDSYFKGSEVLNDLLQPAVVALAYPLYEQLHQIRARWKSIITICFIGSVVA
                     MVTGTSVALLMGASPEIAASILPKSVTTPIAMAVGGSIGGIPAISAVCVIFVGILGAV
                     FGHTLLNAMRIRTKAARGLAMGTASHALGTARCAELDYQEGAFSSLALVLCGIITSLI
                     APFLFPIILAVMG"
     gene            complement(2797..3195)
                     /gene="yohJ"
                     /locus_tag="ACHKBL_000718"
     CDS             complement(2797..3195)
                     /gene="yohJ"
                     /locus_tag="ACHKBL_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HFO5817633.1"
                     /translation="MSKTLNIIWQYLRAFVLIYACLYAGIFIASLLPVTIPGSIIGML
                     ILFVLLALQILPAKWVNPGCYVLIRYMALLFVPIGVGVMQYFDLLRAQFGPVVVSCAV
                     STLVVFLVVSWSSQLVHGERKVVGQKGSEE"
     gene            complement(3326..4234)
                     /locus_tag="ACHKBL_000719"
     CDS             complement(3326..4234)
                     /locus_tag="ACHKBL_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFO5817634.1"
                     /translation="MANWAQKLKLHHLQMLVALGEQGNLTHVARMMNITQPALSKWLS
                     QLEDEIGLTLFERHSKGLRPTEGGKLLLQHAQRLINDMERSQHELARFKQGGLVGSLK
                     IGCSPVATDCVSQAILHLLKEMPTLHLNIEEKVMTPLLQDLLAGSVDVVVGRIGGRAL
                     QLPLNYRVLYTEPVCFVARPEHPLAKYATLSWNDLANWRWIVWPTGTPIRISIDNALV
                     DHGVMLPENTIESASMNVSTNLLQSSDMISILSLRLAQRYASQGQLVILNLPKIEQKG
                     SVGVFWRNNETPSYALSQFLHFLQQN"
     gene            4361..5719
                     /locus_tag="ACHKBL_000720"
     CDS             4361..5719
                     /locus_tag="ACHKBL_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO5817635.1"
                     /translation="MTQRRDLQALIDAAPVGKMQWRVIICCFLVVMLDGFDTAAIGFI
                     APDIRTHWQLTAGDLAPLFGAGLLGLTAGALLCGPLSDRFGRKRVIELCVFLFGALSL
                     ASAFSPDLQTLVFLRFLTGLGLGGAMPNTITMTSEYLPARRRGALVTLMFCGFTLGSA
                     FGGIVSAQLVPVIGWHGILVLGGVLPLMLFVALLVVLPESPRWQVRRQLPQAVIAKTV
                     SAIIRERYVDTHFYLIESASVTKGSIRQLFMGRQLPITLMLWVVFFMSLLIIYLLSSW
                     MPTLLNHRGIDLQHASWVTAAFQIGGTLGALALGVLMDKFNPFRVLALSYAIGAVCIV
                     MIGLSQDGLWLMALAIFGTGIGISGSQVGLNALTATLYPTQSRATGVSWSNAVGRCGA
                     IVGSLSGGVMMAMNFSFDTLFFIIAVPAAISAVMLTQLITVVRQSTSVPDSLPHAGVV
                     NE"
     gene            5731..6759
                     /gene="gtdA"
                     /locus_tag="ACHKBL_000721"
     CDS             5731..6759
                     /gene="gtdA"
                     /locus_tag="ACHKBL_000721"
                     /EC_number="1.13.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047922 - gentisate 1,2-dioxygenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gentisate 1,2-dioxygenase"
                     /protein_id="HFO5817636.1"
                     /translation="MTDNNQNSREQFYQHISGQNLTPLWESLHHLVPKTPNANCAPAY
                     WNYQEIRPLLLESGSLIGAKEAVRRVLVLENPALRGQSSITATLYAGLQLIMPGEVAP
                     SHRHNQSALRFIVEGKGAFTAVDGERTSMNEGDFILTPQWRWHDHGNPGDEPVIWLDG
                     LDLPLVNTLGCGFAEDYPEEQQPVTRKEGDYLPRYAANMLPLRHQTGNSSPIFNYRYD
                     RSREVLHDLTRLGDADEWDGYKMRYVNPVTGGYPMPSMGAFLQLLPKGFASRVARTTD
                     STIYHVVEGSGQVTIGNETFSFSAKDIFVVPTWHGVSFQTTQDTVLFSFSDRPVQEAL
                     GLFREARY"
     gene            6775..7476
                     /locus_tag="ACHKBL_000722"
     CDS             6775..7476
                     /locus_tag="ACHKBL_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HFO5817637.1"
                     /translation="MTQYVFEPQAPVTVPVVGSDEQFPVRRVYCVGRNYAAHAREMGF
                     DPDREPPFFFCKPADAIVPVAAGETLALPYPAQTDNYHYEIELVVAIGKKGSDIPLEN
                     AHEYVWGYATGLDMTRRDRQMEMRQMGRPWEIGKAFDLSAPIAPLHKASETGGIDNAP
                     IWLQVNGEDHQRSDIRHLIWSVNETISYLSGFFELQPGDLIFTGTPEGVGSVVKGDVI
                     TGNVEGLTPIAVKIV"
     gene            7484..8128
                     /gene="maiA"
                     /locus_tag="ACHKBL_000723"
     CDS             7484..8128
                     /gene="maiA"
                     /locus_tag="ACHKBL_000723"
                     /EC_number="5.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311055.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016034 - maleylacetoacetate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylacetoacetate isomerase"
                     /protein_id="HFO5817638.1"
                     /translation="MKLYSFFNSSASYRVRIALALKGIDYQTEGVNIRIGQQNELAYR
                     RMNPVGLVPTLLTDEGESLGQSLAIIDWLESHYPQVPLMPQEEPARNKVLEIVYAIAC
                     DIHPLNNLRVLRYLTEELNVSEEDKKRWYAHWIQQGLSAVEQLLRQSQSGQFCVGETP
                     TLADCCLVPQWANALRMNCDLSGYPRCKAVYDACTQLPAFIAAAPENQQDKISA"
     gene            8143..9336
                     /locus_tag="ACHKBL_000724"
     CDS             8143..9336
                     /locus_tag="ACHKBL_000724"
                     /EC_number="1.14.13.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybenzoate 6-monooxygenase"
                     /protein_id="HFO5817639.1"
                     /translation="MTKVTRAVIVGGGIGGAATALSLARQGIKVVLLEKAHEIGEIGA
                     GIQLGPNAFSALDSLGVGEIARQRAVFTDHITMMDAVNAEEVVRIETGQAFRDHFGGP
                     YAVIHRVDIHASVWEAVLTHPDVEYHTSTNVVDIRETEDDVTVFDDRGNSWIGDILIG
                     CDGVKSVVRQSLLGDAPRVTGHVVYRAVIDCADMPEDLRINAPVLWAGPCCHLVHYPL
                     RGGKQYNLVVTFHSRQQEEWGVKDGSKEEVLSYFEGIHPRPRQMLDKPTSWRRWSTAD
                     REPVAKWGTKRITLVGDAAHPVAQYMAQGACMALEDAVTLGKALAQCEGDAAQAFALY
                     ESVRIPRTARIVWSTREMGRVYHAAGVERQVRNLLWKGKSQAEFYRGMEWLYGWKEDN
                     CLQPR"
     gene            9398..10345
                     /gene="dusC"
                     /locus_tag="ACHKBL_000725"
     CDS             9398..10345
                     /gene="dusC"
                     /locus_tag="ACHKBL_000725"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
                     /protein_id="HFO5817640.1"
                     /translation="MRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVK
                     VFHRICPELQNASRTPAGTLVRVQLLGQFPQWLAENAARAVELGSWGVDLNCGCPSKT
                     VNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQA
                     GATELVVHGRTKEQGYRAEHIDWQAIGEIRQRLNIPVIANGEIWDWQSAQQCMTISGC
                     DAVMIGRGALNIPNLSRVVKYNEPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQW
                     LSYLRKEYDEATELFQHVRVLNNSPDIARAIQAIDIEKL"
     gene            complement(10718..10801)
                     /gene="yohP"
                     /locus_tag="ACHKBL_000726"
     CDS             complement(10718..10801)
                     /gene="yohP"
                     /locus_tag="ACHKBL_000726"
                     /inference="COORDINATES: protein motif:HMM:NF011345.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YohP"
                     /protein_id="HFO5817641.1"
                     /translation="MKIILWAVLIIFLIGLLVVTGVFKMIF"
     gene            11025..12461
                     /gene="mdtQ"
                     /locus_tag="ACHKBL_000727"
     CDS             11025..12461
                     /gene="mdtQ"
                     /locus_tag="ACHKBL_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug resistance outer membrane protein
                     MdtQ"
                     /protein_id="HFO5817642.1"
                     /translation="MNRDSFYPAIACFPLLLMLAGCAPMHETRQALSQHTPAVQVDTA
                     LPTALKNGWPDSQWWLEYHDNQLTSLINNALQNAPVMQVAEQRIQLAEAQAKAIATQD
                     GPQLDFSADMERQKMSAEGLMGPFALNDPAAGTTGPWYTNGTFGLTAGWHLDIWGKNR
                     AEVTARLGTVKARAAEREQTRQLLAGSVARLYWEWQTQAALNTVLQQIEKEQNTIIAT
                     DRQLYQNGITSSVEGVETDINASKTRQQLNDVAGKMKIIEARLSALTNHQTKALKLNP
                     VALPKVASQLPDELGYSLLARRADLQAAHWYVESSLSTIDAAKAAFYPDINLMAFLQQ
                     DALHLSDLFRHSAQQMGVTAGLTLPIFDSGRLNANLDIAKAESNLSIASYNKAVVDAV
                     NDVARAASQVQTLAEKNQHQAQIERDALRVVGLAQARFNAGIIAGSRVSEARIPALRE
                     RANGLLLQGQWLDASIQLTGALGGGYKR"
     gene            12514..13275
                     /gene="yohF"
                     /locus_tag="ACHKBL_000728"
     CDS             12514..13275
                     /gene="yohF"
                     /locus_tag="ACHKBL_000728"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFO5817643.1"
                     /translation="MAQVAIITASDSGIGKECALLLAQQGFDIGITWHSDEEGAKDTA
                     REVVSHGARAEIVQLDLGKLPEGAQALEKLIQRLGRIDVLVNNAGAMTKAPFLDMAFD
                     EWRKIFTVDVDGAFLCSQIAARQMVKQGQGGRIINITSVHEHTPLPDASAYTAAKHAL
                     GGLTKAMALELVRHKILVNAVAPGAIATPMNGMDDSDVRPDAEPSIPLRRFGTTHEIA
                     SLVVWLCSEGANYTTGQSLIVDGGFMLANPQFNPE"
     gene            complement(13394..13972)
                     /gene="yohD"
                     /locus_tag="ACHKBL_000729"
     CDS             complement(13394..13972)
                     /gene="yohD"
                     /locus_tag="ACHKBL_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFO5817644.1"
                     /translation="MDLNTLISQYGYAALVIGSLAEGETVTLLGGVAAHQGLLKFPLV
                     VLSVALGGMIGDQVLYLCGRRFGGKLLRRFSKHQDKIERAQKLIQRHPYLFVIGTRFM
                     YGFRVIGPTLIGASQLPPKIFLPLNILGAFAWALIFTTIGYAGGQVIAPWLHNLDQHL
                     KHWVWLILAVVLVVGVRWWLKRRGKKKPDNQA"
     gene            14142..14729
                     /gene="yohC"
                     /locus_tag="ACHKBL_000730"
     CDS             14142..14729
                     /gene="yohC"
                     /locus_tag="ACHKBL_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014170891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031267 - small GTPase binding [Evidence
                     IEA];
                     GO_process: GO:0006888 - endoplasmic reticulum to Golgi
                     vesicle-mediated transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Yip1 family protein"
                     /protein_id="HFO5817645.1"
                     /translation="MSHVWGLFSHPDREMQVINRENETISHHYTHHVLLMAAIPVICA
                     FIGTTQIGWNFGDGTILKLSWFTGLALAVLFYGVMLAGVAVMGRVIWWMARNYPQRPS
                     LAHCMVFAGYVATPLFLSGLVALYPLVWLCALVGTVALFYTGYLLYLGIPSFLNINKE
                     EGLSFSSSTLAIGVLVLEVLLALTVILWGYGYRLF"
     gene            14903..15835
                     /gene="pbpG"
                     /locus_tag="ACHKBL_000731"
     CDS             14903..15835
                     /gene="pbpG"
                     /locus_tag="ACHKBL_000731"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HFO5817646.1"
                     /translation="MPKFRVSLFSLALMLAVPLAPQAVAKTAAATTASQPEIASGSAM
                     IVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVY
                     SRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTR
                     FVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNH
                     LVYRDNWNIQLTKTGFTNAAGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTW
                     IETGKVMPVPAAALSYKKQKAAQMAAAGQTAQND"
     gene            complement(15874..17589)
                     /gene="dld"
                     /locus_tag="ACHKBL_000732"
     CDS             complement(15874..17589)
                     /gene="dld"
                     /locus_tag="ACHKBL_000732"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_process: GO:0006089 - lactate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HFO5817647.1"
                     /translation="MSSMTTTDNKAFLNELARLVGHSHLLTDPAKTARYRKGFRSGQG
                     DALAVVFPGSLLELWRVLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDIVIISTL
                     RLDKLHVLGKGEQVLAYPGTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSG
                     GSLVQRGPAYTEMSLFARINEDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRH
                     DGRHAHDYDYVHRVRDIEADTPARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQ
                     QVFYIGTNQPEVLTEIRRHILANFENLPVAGEYMHRDIYDIAEKYGKDTFLMIDKLGT
                     DKMPFFFNLKGRTDAMLEKVKFFRPHFTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHL
                     LLKMAGDGVGEAKSWLVDYFKQAEGDFFVCTPEEGSKAFLHRFAAAGAAIRYQAVHSD
                     EVEDILALDIALRRNDTEWYEYLPPEIDSQLVHKLYYGHFMCYVFHQDYIVKKGVDVH
                     ALKEQMLELLQQRGAQYPAEHNVGHLYKAPETLQKFYRENDPTNSMNPGVGKTSKRKN
                     WQEVE"
     gene            17785..20082
                     /gene="bglX"
                     /locus_tag="ACHKBL_000733"
     CDS             17785..20082
                     /gene="bglX"
                     /locus_tag="ACHKBL_000733"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HFO5817648.1"
                     /translation="MKWLCSVGIAVSLALQPALADDLFGNHPLTPEARDAFVTELLKK
                     MTVDEKIGQLRLISVGPDNPKEAIREMIKDGQVGAIFNTVTRQDIRAMQDQVMELSRL
                     KIPLFFAYDVLHGQRTVFPISLGLASSFNLDAVKTVGRVSAYEAADDGLNMTWAPMVD
                     VSRDPRWGRASEGFGEDTYLTSTMGKTMVEAMQGKSPADRYSVMTSVKHFAAYGAVEG
                     GKEYNTVDMSPQRLFNDYMPPYKAGLDAGSGAVMVALNSLNGTPATSDSWLLKDVLRD
                     QWGFKGITVSDHGAIKELIKHGTAADPEDAVRVALKSGINMSMSDEYYSKYLPGLIKS
                     GKVTMEELDDAARHVLNVKYDMGLFNDPYSHLGPKESDPVDTNAESRLHRKEAREVAR
                     ESLVLLKNRLETLPLKKSATIAVVGPLADSKRDVMGSWSAAGVADQSVTVLTGIKNAV
                     GENGKVLYAKGANVTSDKGIIDFLNQYEEAVKVDPRSPQEMIDEAVQTAKQSDVVVAV
                     VGEAQGMAHEASSRTDITIPQSQRDLIAALKATAKPLVLVLMNGRPLALVKEDQQADA
                     ILETWFAGTEGGNAIADVLFGDYNPSGKLPMSFPRSVGQIPVYYSHLNTGRPYNADKP
                     NKYTSRYFDEANGALYPFGYGLSYTTFTVSDVKLSAPTMKRDGKVTASVQVTNTGKRE
                     GATVVQMYLQDVTASMSRPVKQLKGFEKITLKPGETQTVSFPIDIEALKFWNQQMKYD
                     AEPGKFNVFIGTDSARVKKGEFELL"
     gene            20231..21148
                     /gene="osmF"
                     /locus_tag="ACHKBL_000734"
     CDS             20231..21148
                     /gene="osmF"
                     /locus_tag="ACHKBL_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein OsmF"
                     /protein_id="HFO5817649.1"
                     /translation="MPLSKLWAGSLVLLAAVSLPLQAASPVKVGSKIDTEGALLGNII
                     LQVLESHGVPTVNKVQLGTTPVVRGAITSGELDIYPEYTGNGAFFFKDENDAAWKNAG
                     QGYEKVKKLDAEQNKLIWLTPAPANNTWTIAVRQDVAEKNKLTSLADLSRYLKEGGTF
                     KLAASAEFIERADALPAFEKAYDFKLDQDQLLSLAGGDTAVTIKAAAQQTSGVNAAMA
                     YGTDGPVAALGLQTLSDPQGVQPIYAPAPVVRESVLKEYPQMAQWLQPVFASLDAKTL
                     QQLNASIAVEGLDAKKVAADYLKQKGWTK"
     gene            21155..22312
                     /gene="yehY"
                     /locus_tag="ACHKBL_000735"
     CDS             21155..22312
                     /gene="yehY"
                     /locus_tag="ACHKBL_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehY"
                     /protein_id="HFO5817650.1"
                     /translation="MTYLRINPVLALLLLLTAIAAALPFISYAPNRLVSGEGRHLWQL
                     WPQTIWMLVGVGCAWLTACVIPGKKGSICALILAQFVFVLLVWGAGKAATQLAQNGSA
                     LARTSLGSGFWLAAALALLACSDAIRRISTHLLWRWLLHMQIAIIPLWLLYSGTLNDL
                     SLMKEYANRQDVFDDALAQHLTLLFGAVLPALVIGVPLGVWCYFSTARQGAIFSLLNV
                     IQTVPSVALFGLLIAPLAALVTAFPWLGKLGISGTGMTPALIALVLYALLPLVRGVVV
                     GLNQIPRDVLESARAMGMSGAQRFLHVQLPLALPVFLRSLRVVMVQTVGMAVIAALIG
                     AGGFGALVFQGLLSSAIDLVLLGVIPVIVLAVLIDALFDLLIALLKVKRND"
     gene            22305..23231
                     /gene="yehX"
                     /locus_tag="ACHKBL_000736"
     CDS             22305..23231
                     /gene="yehX"
                     /locus_tag="ACHKBL_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter ATP binding
                     protein YehX"
                     /protein_id="HFO5817651.1"
                     /translation="MIEFSHVSKLFGAQKAVNDLNLNFQEGSFSVLIGTSGSGKSTTL
                     KMINRLVEHDSGVIRFAGEEIRSLPVLELRRRMGYAIQSIGLFPHWSVAQNIATVPQL
                     QKWSRARIDDRIDELMALLGLEPNLRERYPHQLSGGQQQRVGVARALAADPQVLLMDE
                     PFGALDPVTRGALQQEMTRIHRLLGRTIVLVTHDIDEALRLAEHLVLMDHGEVVQQGN
                     PLTMLTRPANDFVRQFFGHSELGVRLLSLRSVADYVRREERAEGEALAEEMTLRDALS
                     LFVARGCEVLPVVNTQGEPCGTLHFQDLLVEA"
     gene            23236..23967
                     /gene="yehW"
                     /locus_tag="ACHKBL_000737"
     CDS             23236..23967
                     /gene="yehW"
                     /locus_tag="ACHKBL_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehW"
                     /protein_id="HFO5817652.1"
                     /translation="MKMLRDPLFWLIALFVALIFWLPYSQPLFAALFPQLPRPVYQQE
                     SFAALALAHFWLVGISSLFAVIIGTGAGIIVTRPWGAEFRPLVETIAAVGQTFPPVAV
                     LAIAVPVIGFGLQPAIIALILYGVLPVLQATLAGLGAIDASVTEVAKSMGMSRGQRLR
                     KVELPLAAPVILAGVRTSVIINIGTATIASTVGASTLGTPIIIGLSGFNTAYVIQGAL
                     LVALAAIIADRLFERLVQALSQHAK"
     gene            complement(23948..24055)
                     /gene="yohO"
                     /locus_tag="ACHKBL_000738"
     CDS             complement(23948..24055)
                     /gene="yohO"
                     /locus_tag="ACHKBL_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohO"
                     /protein_id="HFO5817653.1"
                     /translation="MRIAKIGVIALFLFMALGGIGGVMLAGYTFILRAG"
     gene            complement(24115..24846)
                     /gene="mlrA"
                     /locus_tag="ACHKBL_000739"
     CDS             complement(24115..24846)
                     /gene="mlrA"
                     /locus_tag="ACHKBL_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MlrA"
                     /protein_id="HFO5817654.1"
                     /translation="MALYTIGEVALLCDINPVTLRAWQRRYGLLKPQRTDGGHRLFND
                     ADIDRIREIKRWIDNGVQVSKVKMLLSNENVDVQNGWRDQQETLLTYLQSGNLHSLRT
                     WIKERGQDYPAQTLTTHLFIPLRRRLQCQQPTLQALLAILDGVLINYIAICLASARKK
                     QGKDALVVGWNIHDTTRLWLEGWIASQQGWRIDVLAHSLNQLRPELFEGRTLLVWCGE
                     NRTSAQQQQLTSWQEQGHDIFPLGI"
     gene            25068..26753
                     /gene="btsS"
                     /locus_tag="ACHKBL_000740"
     CDS             25068..26753
                     /gene="btsS"
                     /locus_tag="ACHKBL_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HFO5817655.1"
                     /translation="MYDFNLVLLLLQQMCVFLVIAWLMSKTPLFIPLMQVTVRLPHKF
                     LCYIVFSIFCIMGTWFGLHIDDSIANTRAIGAVMGGLLGGPVVGGLVGLTGGLHRYSM
                     GGMTALSCMISTIVEGLLGGLVHSILIRRGRTDKVFNPITAGAVTFVAEMVQMLIILA
                     IARPYEDAVRLVSNIAAPMMVTNTVGAALFMRILLDKRAMFEKYTSAFSATALKVAAS
                     TEGILRQGFNEVNSMKVAQVLYQELDIGAVAITDREKLLAFTGIGDDHHLPGKPISST
                     YTLKAIETGEVVYADGNEVPYRCSLHPQCKLGSTLVIPLRGENQRVMGTIKLYEAKNR
                     LFSSINRTLGEGIAQLLSAQILAGQYERQKAMLTQSEIKLLHAQVNPHFLFNALNTIK
                     AVIRRDSEQASQLVQYLSTFFRKNLKRPSEFVTLADEIEHVNAYLQIEKARFQSRLQV
                     NIAIPQELSQQQLPAFTLQPIVENAIKHGTSQLLDTGRVAISARREGQHLMLEIEDNA
                     GLYQPVTNASGLGMNLVDKRLRERFGDDYGISVACEPDCYTRITLRLPWRDEA"
     gene            26750..27469
                     /gene="btsR"
                     /locus_tag="ACHKBL_000741"
                     /gene_synonym="yehT"
     CDS             26750..27469
                     /gene="btsR"
                     /locus_tag="ACHKBL_000741"
                     /gene_synonym="yehT"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310963.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BtsR"
                     /protein_id="HFO5817656.1"
                     /translation="MIKVLIVDDEPLARENLRVFLQEQSDIEIVGECSNAVEGIGAVH
                     KLRPDVLFLDIQMPRISGLEMVGMLDPEHRPYIVFLTAFDEYAIKAFEEHAFDYLLKP
                     IDEARLEKTLARLRQERSKQDVSLLPENQQALKFIPCTGHSRIYLLQMKDVAFVSSRM
                     SGVYVTSHEGKEGFTELTLRTLESRTPLLRCHRQYLVNLAHLQEIRLEDNGQAELILR
                     NGLTVPVSRRYLKSLKEAIGL"
     gene            27516..27986
                     /gene="yehS"
                     /locus_tag="ACHKBL_000742"
     CDS             27516..27986
                     /gene="yehS"
                     /locus_tag="ACHKBL_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HFO5817657.1"
                     /translation="MLSNDILRSVRYILKANNNDLVRILALGNVEATAEQIAVWLRKE
                     DEEGFQRCPDIVLSSFLNGLIYEKRGKDESAPALEPERRINNNIVLKKLRIAFSLKTD
                     DILAILTEQQFRVSMPEITAMMRAPDHKNFRECGDQFLRYFLRGLAARQHVKKS"
     gene            complement(28028..28489)
                     /gene="yehR"
                     /locus_tag="ACHKBL_000743"
     CDS             complement(28028..28489)
                     /gene="yehR"
                     /locus_tag="ACHKBL_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehR family lipoprotein"
                     /protein_id="HFO5817658.1"
                     /translation="MKAFNKLFSLVVASVLVFSLAGCGDKEESKKFSANLNGTEIAIT
                     YVYKGDKVLKQSSETKIQFASIGATTKEDAARTLEPLSAKYKNIAGVEEKLTYTDTYA
                     QENVTIDMEKVDFKALQGISGINVSAEDAKKGITMAQMELVMKAAGFKEVK"
     gene            complement(28614..30617)
                     /gene="yehQ"
                     /locus_tag="ACHKBL_000744"
     CDS             complement(28614..30617)
                     /gene="yehQ"
                     /locus_tag="ACHKBL_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HFO5817659.1"
                     /translation="MNSLRPELLELTPQALTALSNAGFVKRSLKELENGNVPEISHEN
                     GALIATFSDGVRTQLANGQALKEAQCSCGASGMCRHRVMLVLSYQRLCATTQPTEKEE
                     EWDPAIWLEELATLPDATRKRAQALVAKGITIELFCTPGEIPSARLPMSDVRFYSRSS
                     IRFARCDCIDGTLCEHVVLAVQAFVEAKTQQAEFTHLIWQMRSEHVTSSDDPFASEEG
                     NACRQYVQQLSQALWLGGISQPPIHYEAAFSRAQQAAERCNWRWVSESLRQLRASVDA
                     FHARASHYHAGKCLCQLAALTSRLNSAQEIARRDSVGEVPPMPWRTVVGSGIAGEAKL
                     DHLRLVSLGMRCWQDIEHYGLRIWFTDPDTGSILHLSRSWPRSEQENSPAATRRLFSF
                     QAGALAGGQIVSQAAKRSADGELLLATRNRLSSVVPLSPDAWQMLSAPLRQPGIVALR
                     EYLRQRPPACIRPLNQVDNLFILPVAECISLGWDSSRQTLDAQVISGEGEDNLLTLSL
                     PASASAPYAVERMAALLQQTDDPVCLVSGFVSFVEGQLTLEPQVMMTKTRAWALDAET
                     APVVASLPSASVLPVPSTAHQLLMRCQALLIQLLHNGWRYQEHSAIGQAELLANDLTA
                     VGFYRLAHVLGQFRNTESEARVEAMNNGVLLCEQLFPMLQQQG"
     gene            complement(30614..31750)
                     /gene="yehP"
                     /locus_tag="ACHKBL_000745"
     CDS             complement(30614..31750)
                     /gene="yehP"
                     /locus_tag="ACHKBL_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HFO5817660.1"
                     /translation="MSELNDLLTTRELQRWRLILGEAAETTLCGLDDNARQIDHALEW
                     LYGRDPERLQRGERSGGLGDSNLTTPEWINSIHTLFPQRVIERLESDAVLRYGIEDVV
                     TNLDVLERMQPSESLLRAVLHTKHLMNPEVLAAARRIVRQVVEEIMARLAKEVRQAFS
                     GVRDRRRRSFIPLARNFDFKSTLRANLQHWHPQHGKLYIESPRFNSRIKRHSDQWQLV
                     LLVDQSGSMVDSVIHSAVMAACLWQLPGIRTHLVAFDTSVVDLTADVADPVELLMKVQ
                     LGGGTNIASAVEYGRQLIEQPAKSVIILVSDFYEGGSSSLLTHQVKKCVQSGIKVLGL
                     AALDSTATPCYDRDMAQALVNVGAQIAAMTPGELATWLAENLQS"
     gene            complement(31743..34022)
                     /gene="yehM"
                     /locus_tag="ACHKBL_000746"
     CDS             complement(31743..34022)
                     /gene="yehM"
                     /locus_tag="ACHKBL_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5682 family protein"
                     /protein_id="HFO5817661.1"
                     /translation="MSEPLIVGIRHHSPACARLVKSLIESQRPRYVLIEGPADFNDRV
                     DELFLAHQLPVAIYSYCQYQDGAAPGRGAWTPYAEFSPEWQALQAARRIQAQTYFIDL
                     PCWAQSEEEDDSPDTQEESQTLLLRATRMDNSDTLWDHLFEDESQQTALPSALAHYFA
                     QLRGDSPGDALNRQREDFMARWIGWAMQQNNGDVLVVCGGWHASALAKMWRECPQEIN
                     TPELPSLADAVTGCYLTPYSEKRLDVLAGYLSGMPAPVWQNWCWRWGLQQAGEQLLKT
                     VLTRLRQHHLPASTADMAAAHLHAMALAQLRGHTLPLRTDWLDAIAGSLIKEALNAPL
                     PWSYRGVIHPDTDPILLTLIDTLAGDGFGKLAPSTPQPPLPKDVTCELERTGISFPAE
                     LTLNRFTPDGLAQSQVLHRLVILEIPGVVRQQGSTLTLAGNGEERWKLTRPLSQHAAL
                     IEAACFGATLQEAARNKLEADMLDAGGIGSITTCLSQAALAGLASFNQQLLEQLTLLI
                     AQENQFAEMGQALEVLYALWRLDEISGMQGAQILQTTLCAAIDRTLWLCESNGRPDEK
                     EFHAHLHSWQALCHILRDLHSGVNLPGVSLSAAVALLERRSQAIHAPALDRGAALGAL
                     MRLEHPNASAEAALTMLVQLSPTQSGEALHGLLALARHQLACQPVFIAGFSSHLNQLS
                     DADFINALPDLRAAMAWLPPRERGTLAHQVLEHYQLVQLPVSALQMPLHCPPQDIAHH
                     QQLEQQALASLQHWGVFHV"
     gene            complement(34033..35121)
                     /gene="yehL"
                     /locus_tag="ACHKBL_000747"
     CDS             complement(34033..35121)
                     /gene="yehL"
                     /locus_tag="ACHKBL_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HFO5817662.1"
                     /translation="MSPQNNHLQRPPAAVLYADELAKLKQNDHAPCPPGWQLSLPAAR
                     AFILGDSAQNISRKVVISPSAVERMLVTLATGRGLMLVGEPGTAKSLLSELLATAISG
                     DAGLTIQGGASTTEDQIKYGWNYALLINHGPSTEALVPAPLYQGMRDGKIVRFEEITR
                     TPLEVQDCLLGMLSDRVMTVPELTGEASQLYAREGFNIIATANTRDRGVNEMSAALKR
                     RFDFETVFPIMDFAQELELVASASARLLAHSGIPHKVPDAVLELLVRTFRDLRANGEK
                     KTSMDTLTAIMSTAEAVNVAHAVGVRAWFLANRAGEPADLVDCIAGTIVKDNEEDRAR
                     LRRYFEQRVATHKEAHWQAYYQARHRLP"
BASE COUNT         8306 a         9132 c         9762 g         8361 t
ORIGIN      
        1 aacaatcatg ccgatagata aaattccgaa gccgatatat gccaccatat aagcgaagaa
       61 gagtccggga ttttttgctc gccagacata caccggttcc ggtgcatttt taacccgttt
      121 atcaatcgcg cctgcgcgcc agcctttcca tgcttcgcgg aaacaaaata ccgggataag
      181 gaaaaaaacg gcaacgacaa aaaactgctg tacatccata actactttcc ttgtttcttt
      241 tgtaattcaa gtaactgttc gggtgtgacg gcgggatacc cctgcgcatc ttcaggcacc
      301 tggtgcatag ccggaatagg gaccagcggc cctaaaaaac gcggttcacg tttaaaaata
      361 taaaggtcag ccagcgcacc gaaacgggcg gcaaattcgc gaatacgtac tgacagcata
      421 tctttcggtg acggtacggc ataacactga gcctgaatcc ccatatgcag cgcaataaat
      481 aatgcccgct cacagtggaa acgttgggtg ataataatga aatcattagt atcgaaaact
      541 ttgcgtgtac gcacgatgga atccagcgta cgaaagcctg cgtaatcgag aacaatatct
      601 gatgggtcga caccagcagc gattaaatct ttgcgcatgg tcatcggctc attataactt
      661 tgcaatgcgt tatcgccgct cagtaataga taatttacct taccgctgtt ataggcatta
      721 atcgctcctt gaatgcggta gcgataatac tgattaatta cgccagtacg ataatatttt
      781 gctgttccga gcaccacacc gacctggcgg taggggagat cctgcaattc gtcgtagata
      841 taaggcgcgg ttttccagct catccagcga tcgaggccga gcacagtcaa cagcagcaag
      901 ccgatcagga ctaacaggct gaggaacacg cgctttaaca tgcaggagac tcgatctaag
      961 gaaaatttcc atcaggctac tttacccgct ggttttgcgc aagaaacggc aaactgtttg
     1021 ccgggatttt cgacaccagg agagggctgg caagccggac aaggcgttca cgccgcatcc
     1081 ggcaccaggc ttaagcgaga agcacgcggt cgatattgtg acagccgaga gctttcagtg
     1141 ttgcggaggt ggcatcccac tgaatcaacg gcgcatcggc tttttctgcc agaaccgcgc
     1201 gctggatatc cgggtaatca taacccttga gattcaacag aattaacgcc ccctgcaacg
     1261 gtggcagagt cgggttgaat gcggcgtttt cagcgtagct gccgctgaaa atacgaccgt
     1321 ctttacattc cagcgcgaca ccgcttggcg acttactgta aggcatgtgc gaacggtttg
     1381 ccgccgcaat cgctgcctga gaaagcgcat cacccgtcag cgcatagccg tgatcttgct
     1441 cgtccatcag cagcgtttta atctccagat ctttcggccc aaaggcatcc ggcagatagt
     1501 cacgcagcgc gtgtgcctcg cggcccggca gatgaatacg cagatccaga ccgctgttca
     1561 gttcattcat aaactgacgg cagtgaccac aaggcgtata gttaacggtg atggctacaa
     1621 gcgctttttc accactcaac caggcgtggc tgatcgcgct ttgttcggca tgaacggttt
     1681 gctgcattgt cgcaccaata aattccatat tggcaccaaa ataccaggtt ccgctcacac
     1741 cgcgcgcaat tgcgccaaca ttaaaattcg acaatggcgt acgcgcacag gctgccgcca
     1801 gcggaagtag tgcgaatgcc agcgcgtctt cgtccagccc cgttgcgctc ttcagcgatg
     1861 agacttgctc cccggtcaac aaagcgggga agtacttgtc tgccagaata ggttccagtg
     1921 cagattgcaa gttatccgca agttgggcaa aagcggtttg aaaacgtgga tgcatggcgt
     1981 tgcctcataa tcatgtaatg ggataacagt ttacgaaccc gttatggctt tatatgtgat
     2041 ctgaatctca ttaattatgc aacgcgtgaa acttacatca aaaatgcgcg acgcatcgca
     2101 aattttagcc cattactgcc agaataatcg ggaaaaggaa cggtgcgatc agcgaagtaa
     2161 taatcccgca taacaccagc gccagcgaac taaatgcacc ttcctgataa tccagctcgg
     2221 cgcagcgcgc cgtaccgagg gcgtgcgagg ctgtgcccat tgccagaccg cgcgcagctt
     2281 tggtacgaat acgcatcgca ttaagcaatg tatggccaaa cacagcgccg aggatgccga
     2341 cgaaaatcac gcaaactgcg ctgattgccg gaataccgcc aatactgccg ccaaccgcca
     2401 ttgcaatagg cgtggtgact gatttcggca ggattgacgc ggcgatttcc ggtgaagcgc
     2461 ccatcaacaa tgccacagaa gtccccgtca ccatcgcaac cacgctgccg ataaaacaga
     2521 tggtgatgat cgatttccag cgcgcgcgga tctggtgcag ctgctcatat aaaggatagg
     2581 ccagtgcgac aaccgccggt tgaagcagat cgtttagcac ttcgctacct ttaaagtagc
     2641 tgtcgtaaga gatgccggtc agcattaaaa aaggaatgat caccaccatt gctaccagca
     2701 acgggttaag caggggaaac ttataccgtg ccgccagttt gcgggcggca aaaaagacaa
     2761 tcaacgtcag cggtaatgac caccagatat tcgccatcat tcttctgatc ctttctgacc
     2821 tacgacttta cgttcaccgt gcaccagttg tgaactccag ctcaccacca gaaaaaccac
     2881 cagcgtactg actgcacagg aaaccactac cggaccaaac tgtgcgcgga gcaaatcaaa
     2941 atattgcatg acgcctacac caatcggcac aaacaatagc gccatatagc gaatcagtac
     3001 gtagcacccc ggattgaccc atttcgccgg aagaatttgc aaggccagca ggacaaacag
     3061 gatcagcatc ccgatgatgc tgcccggaat ggttaccggt agcagggaag caatgaaaat
     3121 gcctgcatac aggcaggcat aaatcaggac aaaagcacgt aaatattgcc agataatgtt
     3181 cagtgtctta ctcataatca acaccccaga tgagatggat tcatcataca attaattcgt
     3241 aaaacgtgct accgatcaca ttaagaaatc tcagcatagt tgaattttag ggacgtaacc
     3301 tcgggcgagc gcccagttca gcacactaat tctgctgtaa aaagtgcaga aactgactta
     3361 aagcataaga aggcgtttcg ttattgcgcc agaacacacc gacgctgcct ttctgttcta
     3421 tctttggcag gttgagaatc accagttgtc cctggctggc ataacgttgc gccaggcgta
     3481 atgacaaaat agagatcata tcgctgcttt gtaacaggtt ggtactgacg ttcatcgacg
     3541 ccgactcaat cgtgttttct ggcagcatca cgccgtggtc taccagcgca ttatcaatgc
     3601 tgatgcggat gggggtacct gtcggccaga ctatccaccg ccagttagca agatcattcc
     3661 agctgagggt ggcgtatttc gccagtggat gctcaggacg ggcgacaaaa cacaccggct
     3721 cggtgtagag cacgcgataa ttcagcggca gctgcaacgc gcgaccgcca attcgcccaa
     3781 ccaccacatc gacagaacct gccaacagat cttgcagcaa tggggtcatc accttctctt
     3841 cgatattcag gtgtagagtt ggcatctcct tgagcaaatg caaaatcgcc tgggatacgc
     3901 agtcagtggc gacgggagaa cagccaattt tcagactgcc gaccagtcca ccttgtttaa
     3961 aacgggccag ttcatgttgc gagcgctcca tgtcattgat taaccgttgg gcatgttgca
     4021 ataacaattt gccgccttca gtaggacgaa gtcctttgct gtgccgctca aagagtgtga
     4081 ggccgatctc atcttcgagt tgtgacaacc atttcgacag cgcaggctgg gtgatattca
     4141 tcatccgcgc gacatgggtc agattgccct gttcacccag cgccaccagc atttgcaggt
     4201 gatgaagctt tagtttctgc gcccagtttg ccatttgcca taacctccag gttatctctc
     4261 tgtccgaaat gccattgtac atttcatctc tttaatcaca atatcgcaac atacataaaa
     4321 caaatgaaac atctacctta cctgcactga ggcatttact atgactcaac gacgtgattt
     4381 acaagcactg atcgatgctg ctccggtcgg caagatgcag tggcgcgtta tcatctgctg
     4441 ttttctggtg gtaatgctcg atggttttga caccgcggct atcggcttta tcgcccctga
     4501 tattcgcact cactggcaac tcaccgcagg cgatctcgcc ccgctttttg gcgcgggtct
     4561 gctggggtta accgcaggcg cactgctttg tggtccgttg tccgatcgct ttggccgcaa
     4621 acgggtgatt gagctgtgcg tctttttgtt tggcgcgtta agcctcgcct cggctttctc
     4681 accggatctg caaacgctgg tattcctgcg tttcctcacc ggccttgggc tgggtggcgc
     4741 gatgccaaat acaattacca tgacctcgga atatctgcct gcccgccgcc gcggcgcgct
     4801 ggtgacgctg atgttttgcg gctttaccct gggttcggcg tttggcggga ttgtcagcgc
     4861 gcagctggtg ccggtgattg gctggcacgg gatcctggtg ttgggtggcg tattaccgct
     4921 gatgctgttt gttgcactgc tggtggttct gccggaatcg ccacgctggc aggtgcgccg
     4981 ccagctaccc caggcggtga ttgcgaaaac cgtcagcgcc atcatccgcg agcgttatgt
     5041 cgatacccac ttttatctca ttgaatcagc ctccgttaca aaaggcagca ttcgtcaact
     5101 gtttatgggg cgtcagttac ccatcacgtt gatgctgtgg gtggtgttct ttatgagctt
     5161 gctgattatt tacctgctct caagctggat gccaacgctg ctcaaccatc gcggcatcga
     5221 cctgcaacat gcctcctggg ttaccgccgc gttccagatt ggcggtacgt tgggcgcgct
     5281 ggcgctgggc gtgcttatgg ataagttcaa tcctttccgc gtactggcgc tgagctatgc
     5341 cataggcgct gtatgcatcg tgatgattgg cttaagccag gacggattat ggctaatggc
     5401 gctggcgatt ttcggcaccg gcattggcat cagcggatcg caggtggggc tgaatgcgct
     5461 gaccgcaaca ctttatccaa cacaaagtcg cgccacgggc gtgagctggt caaacgccgt
     5521 gggccgctgc ggcgcgattg ttggctcgct gtctggcggc gttatgatgg cgatgaattt
     5581 ctctttcgac accctgtttt tcattatcgc tgtgcccgcg gcgatcagtg cggtgatgtt
     5641 aacccagctg atcaccgtgg ttcgccagtc gacctctgtc cctgactcac tgcctcacgc
     5701 aggtgttgtg aacgaataag gagcatccca atgactgata acaatcaaaa tagccgcgag
     5761 caattttatc agcatatttc cgggcaaaac ctgaccccgc tgtgggagtc actgcaccat
     5821 ctggtgccaa aaacgcccaa cgctaactgt gcgccagcgt actggaatta tcaggagatc
     5881 cgcccgttgc tgctggagag cggcagcctg attggcgcga aagaagccgt gcgccgcgtg
     5941 ctggtgctgg aaaacccggc gctgcgcggg cagtcttcca tcaccgcgac tttatatgcc
     6001 ggtttgcaac tgatcatgcc gggcgaagtg gcaccgagtc atcgccataa ccagtcggcg
     6061 ctgcgtttta ttgttgaagg taaaggggca ttcaccgccg ttgatggcga acgcacgtca
     6121 atgaatgagg gcgattttat cctgaccccg cagtggcgct ggcacgatca cggtaaccct
     6181 ggcgacgaac cggttatctg gctcgatggg ctggatctgc cattagtgaa cactctgggc
     6241 tgcggttttg ccgaagatta cccggaggag caacaaccag taacgcgtaa agagggcgat
     6301 tacctgccgc gttacgctgc caatatgttg ccgttgcgcc atcagaccgg gaactcctcg
     6361 cccatcttta actaccgtta tgaccgtagc cgcgaagtgc tacacgattt aacccgcctg
     6421 ggcgatgccg atgagtggga tggctacaaa atgcgctacg tcaacccggt caccggtggc
     6481 tatccgatgc cgtcgatggg cgcttttctg caactgctgc cgaaagggtt tgcctcacgc
     6541 gtggcgcgta ccactgacag caccatctac catgtggtgg aaggtagcgg gcaggtcact
     6601 atcggcaatg aaaccttcag ctttagtgca aaagatatct tcgtggtgcc gacctggcac
     6661 ggcgtgtcgt tccagaccac gcaagatacc gttttattta gtttttcgga cagaccggta
     6721 caagaagccc tggggctgtt ccgcgaagca cgttattaat ttgggagaga gaaaatgact
     6781 caatacgtat tcgaaccaca agcgcccgtt accgtaccgg ttgttggcag tgatgaacaa
     6841 ttccccgtac gccgcgttta ctgcgtcgga cgtaactatg ccgcccacgc ccgcgaaatg
     6901 ggctttgatc cggatcgtga accgccattc ttcttctgca aaccggcgga tgccattgtt
     6961 ccggttgccg ctggcgaaac gctggcgctg ccgtatccgg cgcagaccga taactaccat
     7021 tatgaaattg aactggtggt ggcgattggt aaaaaaggca gtgatatccc gctggaaaac
     7081 gcccatgaat atgtctgggg ttatgccacg ggtcttgata tgactcgccg tgaccgtcaa
     7141 atggaaatgc gccagatggg ccgcccgtgg gaaatcggca aagcgttcga tctctcggcc
     7201 cccatcgcgc cgctgcacaa agccagcgaa actggcggta ttgataacgc acctatctgg
     7261 ttacaggtta acggcgaaga tcatcagcgc agcgatattc gtcatctcat ctggtcagtg
     7321 aatgaaacca tcagctacct gtcaggcttc tttgaactgc aaccgggcga tctaatcttt
     7381 accggtacgc cggaaggggt tggctcagta gtcaaaggtg atgtgatcac gggcaacgtc
     7441 gagggcttaa cgcctatcgc ggtgaagatt gtctgaggtg atgatgaagc tgtacagttt
     7501 ttttaatagt tcagcgtctt accgggtgcg cattgcgctg gcgctgaagg gtatcgatta
     7561 ccagaccgaa ggggtgaata tccgcattgg tcagcaaaac gaactggcct accggcggat
     7621 gaatccggtc gggctggtgc cgacgctgct gaccgacgag ggcgaatcac tcggacaatc
     7681 gctggcgatt attgactggc tggagagtca ctacccgcaa gttccgctga tgccgcagga
     7741 agaaccggcc cgcaacaagg tgctggaaat tgtctatgcc atcgcctgcg atattcatcc
     7801 attgaataac ctacgcgtgc tgcgctacct gaccgaagag ctgaacgtca gtgaagaaga
     7861 taaaaaacgc tggtatgcgc actggatcca gcaagggtta agcgcggtgg aacagctact
     7921 gcgccagagc cagtcggggc aattttgtgt gggcgagacg ccgacgctgg cagattgctg
     7981 ccttgttcca cagtgggcga acgcgctacg tatgaactgc gatctgagcg ggtatccgcg
     8041 ctgcaaagcg gtatacgacg cctgcacgca gctgccagcg ttcattgccg cagcgccaga
     8101 aaatcaacaa gataaaattt cagcctgacc aggagaacca taatgactaa agtgacacgc
     8161 gcagtaattg tgggaggcgg gatcggcggt gcggcaactg cattgtcact ggcccgccag
     8221 gggatcaaag tggtgctgct ggaaaaggcg catgagatcg gtgagattgg cgcaggtatc
     8281 cagttggggc caaatgcgtt ttcggcgttg gatagcctcg gcgtcggcga aatcgcccgc
     8341 cagcgcgcgg tattcaccga tcacatcacc atgatggatg cggtaaacgc cgaagaagtg
     8401 gtgcgcattg aaaccggtca ggcctttcgc gatcatttcg gcggccctta cgcggtgatc
     8461 caccgggttg atattcacgc ctcggtgtgg gaagcggtac tgacgcatcc ggacgtggaa
     8521 tatcacacct ccaccaacgt cgtggatatt cgcgagacag aagacgatgt caccgttttc
     8581 gacgatcgcg gcaatagttg gatcggcgat attctgattg gctgtgacgg cgtgaagtca
     8641 gtggtgcgcc agagcttatt gggcgatgcg ccgcgtgtca ccgggcatgt ggtctaccgt
     8701 gctgttatcg attgtgccga tatgccggaa gatttgcgca ttaacgcacc agtactgtgg
     8761 gcggggccgt gctgtcactt ggtgcattac cctttacgcg gcggcaagca gtataacctg
     8821 gtggtgacat tccacagccg ccagcaggaa gagtggggcg tgaaggatgg cagtaaagag
     8881 gaagtgctct cttactttga agggattcac ccccgtccgc gccagatgct ggataaaccg
     8941 acctcctggc gacgctggtc aaccgccgac cgtgaaccgg tagcgaagtg gggaacgaag
     9001 cgcattacgc tggtgggcga tgcggcccat ccggtggcgc agtatatggc gcagggtgcc
     9061 tgtatggcgc tggaagacgc ggtaacactg ggtaaggcgc tggcgcagtg tgagggtgat
     9121 gcggcacaag cctttgcgct gtatgagtcg gtacgtattc cgcgcaccgc gcgcatcgtc
     9181 tggtcgaccc gtgaaatggg gcgggtttat catgccgcag gagtagagcg ccaggtacgt
     9241 aacctgttgt ggaaagggaa atcgcaggca gagttttacc gcggcatgga gtggctgtac
     9301 ggctggaaag aagataactg tttgcaacca cgctgagtac tttaatccgc tgaccatagg
     9361 cgctaacata gcgcctcatt tttttgcggg tgatgatatg cgtgtgttac tggcaccgat
     9421 ggagggtgta ctcgactctc tggtgcgtga attgctgacc gaagttaacg actacgatct
     9481 gtgcatcacc gagtttgtcc gcgtggtgga tcaactgctg ccggtaaaag tctttcatcg
     9541 catttgccct gagctacaaa acgccagccg gacaccagcc ggtacgctgg tgcgcgtgca
     9601 gttgttaggt cagttcccac aatggctggc agagaatgcc gcccgtgcgg tcgagttagg
     9661 ttcctggggc gtggatctca attgcggctg cccgtcgaaa acggttaacg gtagcggcgg
     9721 tggggcgacg ttactcaaag atcctgaact catctatcag ggtgcaaaag cgatgcgcga
     9781 agctgtaccg gcgcatttgc ctgtcagcgt aaaagtgcgt ctgggctggg acagcggtga
     9841 gaagaaattt gaaatcgccg atgcggtgca acaggctggc gctacggagc tggtagtaca
     9901 tgggcggaca aaagagcagg gttaccgcgc ggagcatatt gactggcagg cgattggcga
     9961 gattcgccag cggttgaata ttcctgtgat tgccaacggt gaaatctggg actggcagag
    10021 tgcgcaacaa tgcatgacga tcagcggatg cgacgcagtg atgattggtc gcggggcgct
    10081 caatattccc aacctgagcc gggtggtaaa atataacgaa ccgcgaatgc cgtggccgga
    10141 ggtagttgct ttgctgcaaa aatatacccg tctggaaaag cagggcgata ccgggttata
    10201 tcacgttgcg cggattaaac agtggttgag ttatttgcgt aaagaatacg atgaagcaac
    10261 ggaattattt cagcatgttc gggtgttgaa taattcccct gatattgcaa gggctattca
    10321 ggcaattgat atcgagaaac tctaagaaac catagtgatg ttttttgaca atgtgttagc
    10381 tctttatcaa taggccgcac ggttgtttgg tggcgtcaaa atcgccgagg cgttccctga
    10441 aggccggggc agcccacatg gatgtgggct gagggcgcgt tttacaggga tgttacctcg
    10501 cgcccgaccc ggtagccgta agggataagc cgagggcacc gcgcagcggc gattttgttc
    10561 gccagagccc gggggtgcag ggggcggcgg cgattggccg ccccctgcgc gctccttgcg
    10621 ccagtgatta tatgaagcaa tgcacatgac aggagcgcaa caggatgaga gactgaaata
    10681 ttctcgttat tttcggatct gatgacatta attaatttta aaatatcatc ttaaatacgc
    10741 cagtcaccac cagtagccca atcaggaaaa taatcaatac agcccagagt ataattttca
    10801 ttggcgttcc tttatttgtg agtgctgaat aagtatagga aaaaacgaag ccctttgccg
    10861 ctatccataa acgattaaga taaatcggaa gatatcattc acttggtgta tatttttctg
    10921 gatatgtcgt tttattgcca ctgatgtatt gccacgcgaa actaaaaaat atatcgcaac
    10981 acacggtaat ctccgcgcac cttttcttac ctgattattc ccccatgaat cgtgattcct
    11041 tttatccagc catcgcctgt tttccgctgt tactgatgct ggccgggtgt gcgcctatgc
    11101 atgaaacccg ccaggcgtta agccagcaca cgcccgctgt gcaagttgac accgcattac
    11161 ccacggcgct gaaaaatggt tggccagaca gccaatggtg gctggagtat cacgataatc
    11221 aactcacctc attaattaat aacgcactac agaacgcacc ggttatgcag gtggctgaac
    11281 agcgcattca gctggctgaa gcccaggcga aggcaattgc tactcaggat ggtccgcagc
    11341 tcgacttttc tgcggatatg gagcggcaaa aaatgtcggc agaaggctta atggggccgt
    11401 ttgctctgaa cgatccggcc gcaggtacga ccggtccgtg gtacaccaac ggtacttttg
    11461 gcttaacggc gggctggcat ctcgatatct ggggaaagaa tcgggcggag gtaactgctc
    11521 gcctgggtac ggttaaagca cgagcggcgg aacgcgagca aacccgccaa ttgctggctg
    11581 gcagcgtagc ccgcctgtac tgggagtggc aaacccaggc ggcgttaaac acggtcttgc
    11641 agcaaatcga aaaagagcag aacaccatta tcgccaccga tcgccagcta tatcagaacg
    11701 ggattacttc ttcagttgaa ggtgtggaaa ccgatattaa tgccagcaaa acccggcagc
    11761 agctcaacga tgtcgcgggg aaaatgaaaa ttattgaggc acggttaagt gcacttacaa
    11821 atcaccagac aaaggcattg aagcttaatc cggtcgcgtt gccgaaagtg gcaagccagc
    11881 ttcctgatga actggggtac tccttactgg cccggcgggc agatttgcag gcggcgcact
    11941 ggtacgttga gtcatcgtta agcactattg atgcggcaaa agcggcattt tatcctgaca
    12001 tcaacctgat ggccttcctg caacaggatg cgttgcactt aagcgatctg ttccgtcatt
    12061 ccgcgcagca aatgggcgtt acggcaggcc tgacgctacc cattttcgat agtggtcgtc
    12121 ttaacgccaa cctcgatatc gcaaaagccg aaagcaactt gtctatcgcc agctacaaca
    12181 aagcggtggt tgacgcggtg aatgacgtgg cgcgggcagc cagtcaggtt cagacactgg
    12241 cggagaaaaa ccagcatcag gcgcaaattg agcgcgatgc cttgcgtgta gtaggtcttg
    12301 cacaggcgcg ctttaacgcg ggcatcattg ccggttcccg cgtcagcgaa gccagaatcc
    12361 cggcgcttcg cgagcgggcc aatggcctgt tattgcaagg gcagtggctg gatgcctcca
    12421 ttcaactcac tggtgcgttg ggcgggggtt acaaacgctg atgatatgcg ccttctatac
    12481 ttaacgttta ttcagcgtta agtggagaac tcgatggcac aggttgcgat tattaccgcc
    12541 tccgattcgg ggatcggcaa agagtgcgcg ttattactgg cgcagcaggg gtttgatatt
    12601 ggcattacct ggcactctga tgaagaaggg gcaaaagata ctgcgcgtga ggtggttagc
    12661 cacggcgcac gtgcggagat cgtgcagctg gatctcggta aactgccaga aggtgcgcag
    12721 gcgctggaga aactcattca acgactgggg cgcatcgatg tgctggtgaa taatgcgggt
    12781 gcaatgacca aagcgccgtt tcttgatatg gcttttgatg agtggcgcaa gatttttacc
    12841 gttgatgtcg acggtgcatt cttatgctcg caaattgcgg ctcgtcagat ggtgaaacaa
    12901 gggcagggcg gtcgcatcat caacattacg tcggtgcatg aacatacgcc gctgccggat
    12961 gccagcgctt acacagcagc taaacatgcg ctcggcgggt taaccaaagc aatggcgctg
    13021 gagttggtca ggcataaaat tttggtgaac gcagtcgcac ctggggcgat cgccacgcca
    13081 atgaacggca tggatgatag cgatgtgagg cccgacgcgg agccttcgat tcccttgcga
    13141 cgttttggca caacgcatga gattgccagc ctggtggtat ggctttgttc ggagggcgca
    13201 aattacacca ccgggcagtc gttgatagtg gatggcggct ttatgttggc gaatccgcag
    13261 ttcaacccag aatagcgcag tgaggtgcgt ggttatttca atgggttagg ccagataaga
    13321 cacgccagcg tcgcatcagg catggaacat ccggtgccgg atgtggcgtg aatgcttatc
    13381 tgtggcaact gctttacgcc tgattatccg gctttttctt cccgcgtcgt ttcagccacc
    13441 agcgcacgcc taccaccaga accacggcca gaatcaacca gacccagtgc ttcaaatgct
    13501 ggtcgagatt gtgcaaccac ggcgcaatca cctgaccacc agcataaccg atagtggtaa
    13561 aaatcaacgc ccaggcaaat gcgccgagaa tattcagcgg cagaaagatt ttcggcggca
    13621 gctggctggc accaatcagc gtcgggccaa tcaccctaaa gccatacata aaacgcgtac
    13681 caatgacaaa cagatacgga tggcgttgga taagtttctg cgcccgctca attttatcct
    13741 gatgtttcga gaaacggcgt aatagcttgc cgccaaagcg ccgcccgcac agataaagca
    13801 cctggtcgcc aatcatgccg ccaagcgcca cagaaagtac caccagcggg aactttaata
    13861 gcccctgatg tgccgcaacg cctcccagca aagtcacggt ttcaccttcc gccaggctac
    13921 cgatcaccag cgcggcataa ccatattgtg agatgagtgt attgagatcc ataggccagt
    13981 ctgtctccct ttgcttgcct gtaagcatac accctgtgaa tagttcacgc tgaaaaagga
    14041 cgtacatcag acggtgcaaa ttacatgagc gaacaaaaaa taatcacaag ctgacttata
    14101 cttgaactgt caggttcatg aactcacaac aagggggcgg tatgagccat gtctggggac
    14161 tgttttccca tcccgatcgt gaaatgcagg tgattaatcg cgaaaacgaa acgatttctc
    14221 accattacac ccaccacgtt ttgctgatgg cggcgattcc ggtgatttgc gccttcattg
    14281 gcactacaca gattggctgg aattttggcg atggcactat cctgaagtta tcctggttta
    14341 ccggactggc gctggctgtc ttgttttacg gcgttatgct ggctggggtt gcggtgatgg
    14401 ggcgggtcat ctggtggatg gcgcgtaatt atccgcagcg tccgtcactt gcgcactgca
    14461 tggtcttcgc gggctacgtt gcaactccgc tgtttttaag cggtctggtg gcgctttacc
    14521 cgctggtctg gttgtgtgcg ctggtcggca cggtggcgct gttttacacc ggctacctgc
    14581 tgtatctcgg tattccgagc ttcctgaata tcaacaaaga ggaaggactt agcttctcca
    14641 gttcgacact ggcgataggc gtgctggtgc tggaagtgct gctcgccctt accgttatac
    14701 tatggggcta cggttatcgt ttgttctaga aagttctttt gcgctgcatt gctggcgtaa
    14761 atgtcagcaa tgcagcattt cctcacgatt cttctttgac gatcgcactt tttgctcgtt
    14821 atgatggcgt cgctagcctc agtaaatcct tactccggcg gtgcgcaacc cgtgcgcgtg
    14881 aaccactatc tgaatgctca tcatgccgaa atttcgagtt tctttattta gcctggccct
    14941 gatgctggct gtgcctttgg caccgcaggc cgttgcgaaa acggcagccg ctaccaccgc
    15001 ttcacaaccg gaaattgcct ccggtagcgc gatgattgtt gatctgaata ccaacaaagt
    15061 gatctattcg aaccacccgg atctggtgcg tccgattgcg tctatcagca aattaatgac
    15121 cgcgatggtt gtgctggatg cacgactgcc gctggatgaa aaactaaaag tggatatcag
    15181 ccagacgccg gagatgaaag gggtctattc gcgcgtacga ctgaacagcg aaatcagccg
    15241 taaagatatg ctgttgctgg cgctgatgtc ttcagaaaac cgcgcggcgg caagccttgc
    15301 gcaccattat cccggtggtt acaaagcctt tattaaggcg atgaatgcga aagcgaaatc
    15361 gctcggaatg aacaacacgc gctttgttga accgaccgga ttgtcggtgc ataacgtttc
    15421 aactgcccgt gacttaacca aactgctcat tgccagcaaa caatatccgt tgattggtca
    15481 gttaagtact accagggaag atatggcaac cttctctaac ccgacgtata cgctgccgtt
    15541 ccgcaatact aaccatctgg tgtatcgcga taactggaat attcagttaa ccaaaacggg
    15601 ctttaccaat gcggcgggcc attgtctggt gatgcgtacg gttatcaata ataaaccggt
    15661 ggcgctggta gtgatggacg cgtttggcaa atatacccat tttgccgatg ccagccgcct
    15721 gcgtacctgg attgagaccg gtaaagtgat gcctgttccg gcagcagcgt tgagctataa
    15781 aaaacaaaaa gccgcccaaa tggcggcggc ggggcagacg gcacagaacg attaaatgaa
    15841 tgacggatgg cgatactctg ccatccgtaa tttttactcc acttcctgcc agtttttccg
    15901 cttactggtt ttaccaaccc ccggattcat actgttggtc ggatcgttct cgcgataaaa
    15961 cttctgcaac gtttccggtg ctttatacaa atgaccgacg ttatgctcgg cagggtattg
    16021 cgcgccgcgc tgctgtagca gttccagcat ctgttctttc aacgcatgca catccacgcc
    16081 ttttttaacg atgtaatcct gatggaagac atagcacata aaatggccgt aatagagctt
    16141 gtgcaccagc tggctgtcga tctccggcgg taaatactca taccactcgg tgtcgttacg
    16201 ccgcagagcg atatccagtg ccagaatgtc ttcgacttca tcggaatgca ccgcctgata
    16261 acgaattgct gcgcccgcag cggcgaaacg gtgtaaaaac gctttgctgc cttcctccgg
    16321 cgtacagaca aagaaatcgc cttcggcctg tttgaaataa tccaccagcc acgatttggc
    16381 ttcaccgacg ccatcgcccg ccatttttaa cagcagatga tgttcgtatt tatcgcgcca
    16441 gtttttcatg cgcggcggta aatggctggg gaacaggtga ccgaattttt gcatcgcacg
    16501 gtcggtaaaa tgcggacgga agaatttcac tttctccagc atcgcatcgg tgcgcccctt
    16561 gagattaaag aagaacggca tcttgtcggt gcctaactta tcaatcatca ggaaggtgtc
    16621 tttgccgtat ttttccgcta tatcgtagat atcccggtgc atatattccc cggcaaccgg
    16681 cagattttcg aaattagcca gaatatgacg gcggatttcg gtcagtactt ccggctggtt
    16741 ggtgccgata taaaacacct gctgattttt ttccgcttcg aaggtatcaa gacgtaccgc
    16801 aaagacggcc agcttcccgg cgcaaccaga agattcaaat aaccgatccg gatcggcgtt
    16861 ataacgtgcg ggcgtgtcgg cttcaatatc acgaacgcgg tggacatagt cataatcgtg
    16921 ggcgtgacga ccatcgtggc gcacatcgtc atctttgatg cgatcgtcat ccagcttgct
    16981 aaggatctgt tccggcgttt cgcccagatc aatccccaga tggttaacca gcgtcagctt
    17041 gccatcttca tttatacgcg cgaataacga catttcggta tacgccgggc cacgttgcac
    17101 cagcgagccg ccggagttgt tacaaatacc gccgatgacc gatgcgccta tacacgatga
    17161 tccaatcacc gagtgcggtt cgcgtcccag cggtttgagg gctttttcca gcgaataaag
    17221 cgtggtgccc ggataggcca gcacctgttc gcccttgccg agaacgtgca gcttgtcgag
    17281 acgcagggtg ctgatgatca cgatatcgcg atcatagtcg ttaccgtttg gcgtcgatcc
    17341 ttcggtcagg cctgtattgg cagcctgcat cagaataatt ttgtcggcgg tgacgcaggc
    17401 tttcagcacc cgccacaatt ccagtagtga gccagggaaa acgacagcca gtgcgtcgcc
    17461 ctgaccagaa cggaagccct tgcgatagcg ggccgttttt gcgggatcgg tgagcaggtg
    17521 tgaatgaccg accagacggg caagttcatt caaaaaggct ttattatcag ttgttgtcat
    17581 ggaagacatt ttccactcct tgtggtggcg aaaaaatatc acaaactaga atagcgcctt
    17641 gtatgacaat tcagcgagtg aatattttga aaattcagac aataaactga gcattctttt
    17701 tctctctcca tcatgcttat ggcaaactac ggtttttgca ccattttcgc tgccacgtcg
    17761 ggcaacaaag gaagaaaaat ccatatgaaa tggctatgtt cagtaggaat cgcggtaagt
    17821 ctggccctgc agccagcact ggcggatgat ttattcggta accatccctt aacgcctgaa
    17881 gcgcgggatg cgttcgtcac cgaactgctt aagaaaatga cggttgatga gaaaattggt
    17941 cagctgcgct taatcagcgt cggcccggat aatccgaaag aggcgatccg cgagatgatc
    18001 aaagacgggc aggttggggc gattttcaac accgtaaccc gtcaggatat ccgcgccatg
    18061 caggatcagg tgatggaatt aagccgcctg aaaattcctc ttttctttgc ttacgacgtg
    18121 ctgcacggtc agcgcacggt gttcccgatt agcctcggtc tggcctcgtc ttttaacctc
    18181 gatgcggtga aaacggtcgg acgtgtctct gcttatgaag cggcagatga tggcctgaat
    18241 atgacctggg caccgatggt cgatgtctcg cgcgatccgc gctggggacg tgcttccgaa
    18301 ggttttggcg aagatacgta tctcacttca acaatgggta aaaccatggt ggaagcgatg
    18361 cagggtaaaa gcccggcaga tcgttactcg gtgatgacca gcgtcaaaca ctttgccgca
    18421 tacggcgcgg tagaaggcgg taaagagtac aacaccgtcg atatgagtcc gcagcgcctg
    18481 tttaatgatt atatgccgcc gtacaaagcg gggctggacg caggcagcgg cgcggtgatg
    18541 gtggcgctga actcgctgaa cggtacgcca gccacctccg actcctggct gctgaaagat
    18601 gttctgcgcg accagtgggg ctttaaaggc atcaccgttt ccgatcacgg cgcaatcaaa
    18661 gagctgatta aacatggcac ggcggcagac ccggaagatg cggtgcgcgt ggcgctgaaa
    18721 tccggcatca acatgagtat gagcgacgag tattactcga agtatctgcc tgggttgatc
    18781 aaatccggca aagtgacgat ggaagagctg gatgacgctg cccgtcatgt actgaacgtt
    18841 aaatatgata tggggttgtt taacgaccca tacagccatt tggggccgaa agagtctgac
    18901 ccggtggata ccaatgccga aagccgcctg caccgcaaag aagcgcgtga agtggcacgc
    18961 gaaagcctgg tgttgctgaa aaaccgtctc gaaacgttac cgctgaaaaa atcagccacc
    19021 attgcggtgg ttggcccgct ggcagacagc aagcgtgacg tgatgggaag ctggtccgca
    19081 gccggtgtcg ccgatcaatc tgttactgtg ctaacaggga ttaaaaacgc cgtcggtgaa
    19141 aacggtaaag tgctgtatgc caaaggggcg aacgttacca gtgacaaagg cattatcgat
    19201 ttcctgaatc agtatgaaga agcggtcaaa gtcgatccgc gttcgccgca agagatgatt
    19261 gatgaagcgg tgcagaccgc gaaacaatct gatgtggtgg tggctgtagt cggtgaagca
    19321 caggggatgg cgcacgaggc ttccagccgt accgatatca ctattccgca aagccaacgt
    19381 gacttgattg cggcgctgaa agccaccgct aaaccgctgg tgctggtgct gatgaacggg
    19441 cgtccgctgg cgctggtgaa agaagatcag caggcggatg cgattctgga aacctggttt
    19501 gcggggactg aaggcggtaa tgcaattgcc gatgtattgt ttggtgatta caacccgtcc
    19561 ggcaagctgc cgatgtcctt cccgcgttct gtcgggcaga tcccggtgta ctacagccat
    19621 ctgaataccg gtcgcccgta taacgccgat aagccgaaca aatacacgtc gcgttatttt
    19681 gatgaagcta acggggcgct gtatccgttc ggctatggtc tgagctacac cactttcacc
    19741 gtctctgatg tgaaactttc cgcgccgacc atgaagcgtg acggcaaagt gaccgccagt
    19801 gtgcaggtga cgaacaccgg taagcgcgaa ggggcgacgg tagtgcagat gtacttgcag
    19861 gatgtgacgg cttccatgag tcgtccggtg aagcagctga aaggctttga gaaaatcacc
    19921 ctgaaaccag gcgaaaccca gaccgtcagc ttcccgattg atatcgaggc gctgaagttc
    19981 tggaatcaac agatgaaata tgacgccgag cctggcaagt tcaatgtctt tatcggcact
    20041 gattccgcac gcgttaagaa aggcgagttt gagttgctgt aatgctttag taagggcgcg
    20101 acgtctgcgc cctttaagta tttatctgat tctgcaactg aatccgccca gacaggctaa
    20161 ccctgtcatt atcaactatg cttttctctt aattcgctga aaacaacagt aaaagaatga
    20221 ggaaagcatc atgccactct caaagctctg ggcaggttca ttggttttgt tggcagccgt
    20281 gagcctgccg ctacaggcgg cttcccccgt taaggtcggt tcaaaaatcg ataccgaagg
    20341 cgcgctgctc ggcaatatca ttttgcaggt gctggaaagt cacggtgttc ctacggtaaa
    20401 taaagtgcaa ctgggaacga ctcccgtggt gcgcggggcg attacttccg gtgaactgga
    20461 tatctatccg gaatataccg gcaatggcgc gtttttcttt aaagatgaaa acgatgcggc
    20521 atggaaaaat gccgggcaag gctacgagaa agtcaaaaaa ctcgatgcag agcaaaacaa
    20581 gttaatctgg ctgacgcccg cgcctgcaaa taacacctgg actatcgccg tgcgtcagga
    20641 tgtggcagag aaaaacaaac tcacttcgct tgccgacctg agtcgttatc tgaaagaggg
    20701 cggcaccttc aaactggcgg cctcggcaga atttattgaa cgcgccgatg ccttacccgc
    20761 ttttgaaaaa gcctacgact ttaaactcga tcaggatcag ttgctgtcac tggctggtgg
    20821 cgacacggcg gtaacgatca aagccgctgc ccagcaaact tctggcgtta atgccgcaat
    20881 ggcttacggc actgacggtc cggtcgcagc gctggggttg caaaccttaa gcgatccgca
    20941 aggcgtgcaa cctatctacg cgcctgcacc agtggttcgt gaatcggtgt tgaaggagta
    21001 tccgcaaatg gcacagtggc tacagccagt cttcgccagc ctcgatgcaa aaacattgca
    21061 gcaactgaat gccagcattg cagttgaagg actggatgcc aaaaaagtgg ctgccgacta
    21121 cctgaaacaa aaagggtgga cgaagtaatt tcccgtgact tatctacgta ttaatcctgt
    21181 tctggcgctg ctgctgttgc tgacggcaat cgcagcggcg ctgccgttta tcagttacgc
    21241 gcctaatcgt ttagtttcgg gtgaggggcg tcatctctgg cagctgtggc cgcaaacgat
    21301 ctggatgctg gtgggcgttg gttgcgcctg gctgaccgcc tgtgttattc ccggtaaaaa
    21361 aggcagcatt tgtgcactca ttctggcgca attcgtcttc gtattgctgg tgtggggagc
    21421 tggaaaggcg gcgacacaac tggcacaaaa tggcagtgcg ctggcgcgta ccagcctcgg
    21481 cagtggtttc tggctggctg cggcgctggc attgctggcc tgtagcgatg ccatccgccg
    21541 aatctccacg catctgctgt ggcgctggtt gttgcatatg cagattgcca ttattccgct
    21601 gtggttgctg tactccggca cgcttaacga tctctcgcta atgaaagaat acgccaaccg
    21661 tcaggatgtg tttgacgacg cgctggcaca acatctgacg ttgctgtttg gtgcggtgct
    21721 gcctgcgtta gtgattggtg tgccgttggg cgtctggtgc tacttttcca ctgcgcggca
    21781 gggagcgatt ttttcgctac tcaatgtcat tcagaccgtg ccttcggtgg cgctctttgg
    21841 cctgttgatt gcgccgcttg ccgcgctggt tacggccttt ccgtggctgg gtaagctcgg
    21901 catatcagga accggaatga cacccgcact gattgcgctg gtgctctatg ccttgctgcc
    21961 gctggtgcgc ggcgtggtag tcggcttgaa ccagatcccg cgcgatgtgc tggagagcgc
    22021 cagagcgatg ggcatgagcg gggcgcagcg attcctgcat gttcagttac cgctggcgtt
    22081 accggtattt ctgcgtagcc tgcgggtggt gatggtgcaa accgtcggca tggcggtgat
    22141 tgcggcgtta atcggcgcag gcggttttgg tgcgctggtt ttccaggggc tgctaagcag
    22201 tgccattgat ttagtgttgc tgggggtgat cccggtaatt gttctggcgg tgctgatcga
    22261 tgcgctgttc gatttgctta tcgcactgct gaaggtgaaa cgtaatgatt gaatttagcc
    22321 atgtcagcaa actgttcggc gcacaaaaag ccgttaacga tctcaatctc aattttcagg
    22381 aggggagttt ttcggtgctg attggtacat caggctccgg taaatccacc accctgaaaa
    22441 tgattaaccg cctggtggag catgacagcg gcgtgatccg ctttgccgga gaagaaattc
    22501 gctcgctgcc agtgctggag ttgcgccgcc ggatgggcta tgccattcaa tctattggcc
    22561 tgtttcccca ctggagcgtg gcgcaaaata ttgccaccgt gccgcaatta caaaaatggt
    22621 cacgggcgcg gatcgacgat cgtatcgacg aattaatggc gctactgggg ctggagccaa
    22681 atttgcgtga gcgttatccg catcagcttt ccggtggtca gcagcaacgt gtgggagtgg
    22741 cgcgcgcact ggctgccgat ccgcaagtct tactgatgga tgaacctttt ggcgcgctgg
    22801 acccggtaac gcgcggcgcg ttgcaacaag agatgacgcg catacaccgt ttgctggggc
    22861 gtaccattgt gctggtcact catgatattg atgaggcgct acgactggca gaacatctgg
    22921 tgttgatgga tcacggtgaa gtggtgcagc agggcaatcc gctgacgatg ctgactcgtc
    22981 cggcgaatga ttttgtccgc cagttttttg ggcatagtga actgggtgtc cgcctgcttt
    23041 cgttacgtag cgtggcggat tacgtgcgtc gcgaagaacg ggcagaaggt gaggcgctgg
    23101 cagaagagat gacgctacgc gatgcgctct ccctgtttgt cgcgcgggga tgtgaagtgc
    23161 tgccggtggt gaacacgcag ggcgagcctt gcggcacgct gcattttcag gatctgctgg
    23221 tggaggcgta agcgtatgaa gatgttgcgc gatccgctgt tctggctcat tgctttgttt
    23281 gtggcactga ttttctggct gccttacagc cagccgctgt ttgctgcctt gttcccacaa
    23341 ctgccacgcc ccgtttatca gcaagaaagt tttgctgctc tggcactggc tcatttctgg
    23401 ctggtgggaa tttcgagttt gtttgcggtg atcattggca ctggtgcagg aattattgtt
    23461 actcgcccgt ggggcgcgga atttcgccca ctggtggaaa ctattgccgc cgttggacag
    23521 acttttccgc ccgtcgcggt gctggcgatt gccgttccgg tgattgggtt tggtctgcaa
    23581 cccgcgatta tcgccttgat cctttatggt gtgctgcccg tcctgcaggc gacacttgcc
    23641 gggctgggag cgattgatgc cagcgtgaca gaagttgcga aaagtatggg aatgagtcga
    23701 ggtcagcgac tgcgtaaggt cgagctaccg ctggcggctc cggtgattct ggcgggtgtg
    23761 cgaacttcgg tgattatcaa cattggtacg gcgacgatcg cctcaacggt aggggccagc
    23821 acgctgggta cgcccatcat catcgggctt agcggattta ataccgcgta tgtgatccag
    23881 ggggcgttac tggtggcact ggcggcgatc attgcagacc gcctgtttga aaggctggtg
    23941 caggcgctta gccagcacgc aaaataaagg tataacctgc gagcatgacg ccaccaattc
    24001 cgcctaacgc cataaacagg aacagggcga tgaccccaat tttagctatg cgcataatgc
    24061 actccttatg ttaacgaaag gattgtacag taaagcgcat ttgttaacga atcattaaat
    24121 gccgagtggg aaaatatcat ggccttgttc ctgccaactg gtgagttgct gctgttgggc
    24181 ggaggttcga ttttcaccgc accacaccag caatgtacgg ccttcgaaca gttcagggcg
    24241 tagttgattg agcgagtggg cgaggacatc aatgcgccat ccttgttgac tggcaatcca
    24301 gccctccagc cacagacggg tggtatcgtg aatattccag ccaaccacca gcgcatcttt
    24361 accctgtttt ttacgtgccg aagccaggca aatggcgatg tagttgatca gtacgccgtc
    24421 gaggatcgcc agcagcgcct ggagagtcgg ttgttggcac tgaagtcgtc ggcgcagagg
    24481 aataaacaga tgcgtggtga gtgtctgggc ggggtaatcc tgaccgcgct ctttgatcca
    24541 cgttcgcagg ctatgcagat tgccgctttg caggtaagtc agtaatgttt cttgctgatc
    24601 gcgccagccg ttctgcacat caacattttc attactgagc agcattttaa ctttgctgac
    24661 ctgcacgccg ttgtcgatcc agcgtttgat ctcgcggatc cggtcaatat cggcatcatt
    24721 gaacagccga tgaccgccgt ctgtccgttg cggtttcagc aatccgtaac gcctctgcca
    24781 cgcgcgtaac gtgacagggt taatatcaca aagcaacgcc acttcaccaa ttgtgtaaag
    24841 cgccatcgtc tcacccttgc tcgcgaggtc ccggtttaac tttagacgca gttttgcgaa
    24901 ccaggtagtt ttgcccgttt tttgtgcatc tatagggtga ttttattttt gccaggcgat
    24961 tttgagtgat cgtactcacg aattctcatt tttctgcaag agttcaaaga aagttaaacg
    25021 caggcaatgt atgttacgcg ttttaaaggg aagtgtggtt tgcgggtatg tacgatttta
    25081 atctggtgtt gctgctgctt cagcagatgt gcgttttttt agtcattgcg tggttaatga
    25141 gtaaaacgcc attattcata ccgttaatgc aggtcacggt tcgtctgccg cataaatttc
    25201 tctgctacat cgtcttttcc atcttctgca tcatgggcac ctggtttggg ctgcatattg
    25261 acgattctat tgccaatacc cgtgcgatag gcgcggtaat gggcggctta ctcggcggtc
    25321 cggtcgtcgg tgggctggtt ggtctgaccg gcgggttaca tcgatattcg atggggggca
    25381 tgaccgcgct aagttgcatg atctcgacca tcgttgaagg gttgctcggc ggcctggtac
    25441 acagcatcct gatccgtcgc gggcgcactg ataaagtctt taaccccatt accgccggtg
    25501 ccgtcacgtt cgtcgctgaa atggtgcaaa tgttgatcat cctggcgatc gcccgacctt
    25561 atgaagatgc ggtgcgtctg gtgagtaata ttgctgcgcc aatgatggtt accaataccg
    25621 tcggcgcggc gctgtttatg cgtatattgc tcgataaacg cgcgatgttt gaaaaataca
    25681 cttcggcttt ttctgccact gcgctaaaag tggctgcctc gacggaaggc attttgcgcc
    25741 aggggtttaa cgaagtgaac agcatgaaag tggcacaggt gctgtatcag gagctggata
    25801 ttggtgcagt cgcgattacc gatcgagaga aattgctggc ctttaccgga attggtgacg
    25861 accaccattt acccggcaaa ccgatttctt cgacttacac cttaaaagcg attgaaaccg
    25921 gtgaagtggt ctacgctgat ggcaacgaag taccttatcg ttgctctttg catccgcaat
    25981 gcaaactggg gtcgacgctg gtcattccgt tgcgtggcga aaatcagcgg gtgatgggca
    26041 ccatcaaatt gtatgaagcc aaaaaccgtt tattcagttc aatcaaccgc acgttgggcg
    26101 aggggattgc gcaactgctt tcggcgcaga tcctcgccgg gcaatatgag cggcaaaaag
    26161 cgatgctcac ccagtcagag atcaaactgc ttcacgccca ggtgaacccc cattttttgt
    26221 ttaatgcgct taacaccatt aaagcggtga tccgccgcga cagcgaacag gccagccagc
    26281 tggtccagta tctttccact tttttccgca aaaacttaaa gcggccttcg gagtttgtta
    26341 ctctcgcaga cgaaattgaa catgtgaacg cttatctgca aattgaaaag gcgcgcttcc
    26401 agtcgcggtt gcaggtcaac attgctattc cgcaagaatt atcccagcag caattgcccg
    26461 cgtttaccct gcaaccgata gtggaaaacg ccattaaaca tgggacatca caactgctgg
    26521 atacagggcg agtggcgatc agcgcccgac gtgaggggca acatttgatg ctggagatcg
    26581 aagacaatgc cggtttgtat caaccggtaa ccaatgccag tgggctgggg atgaatctgg
    26641 tggataagcg tttacgtgaa cggtttggcg atgactatgg gataagcgtc gcctgtgagc
    26701 ctgattgtta cacccgaata acgttacgac taccatggag ggacgaggca tgattaaagt
    26761 cttaattgtc gatgatgaac cgcttgcacg ggagaacctg cgcgtatttt tgcaggagca
    26821 gagcgatatt gaaatcgttg gagagtgttc aaacgccgta gaagggatcg gcgcggtgca
    26881 taaactgcgc ccggatgtgc tgtttctcga tatccagatg ccgcgcatca gtggtctgga
    26941 aatggtgggg atgctcgacc ccgaacatcg accgtatatc gtttttctca ccgcgtttga
    27001 cgaatacgct attaaagcct ttgaagaaca tgcctttgac tatctgctga agccaattga
    27061 tgaagcgcga ctggagaaaa cgctggcgcg tttgcgtcag gaacgcagta agcaggatgt
    27121 ttcgctgtta ccggaaaatc aacaggcgct gaaatttatc ccttgtacgg ggcatagtcg
    27181 gatttattta ctgcaaatga aagatgtggc atttgtcagc agtcggatga gcggtgtcta
    27241 cgttaccagc cacgaaggga aagagggctt taccgaattg acattacgta ccctggaaag
    27301 tcgtacacca ctactgcgct gccatcgtca gtatctggtt aacctcgcgc atttacagga
    27361 gattcgtctg gaagataacg gccaggccga gttgattttg cgtaatggct taaccgtgcc
    27421 ggtcagccgc cgttatctga aaagcttaaa agaggcgatt ggcctgtaaa agactgctaa
    27481 aatggctttt tgcctcatca acacctgaag gcctcatgct aagtaacgat attctgcgca
    27541 gcgtgcgcta cattttgaaa gccaataata atgacctggt gcgtattctg gcgctgggta
    27601 atgtcgaagc caccgcggaa cagatcgccg tctggctacg taaagaagac gaagagggtt
    27661 ttcagcgttg tccggacatt gttttgtcgt cattcctcaa tggcctgatt tatgaaaaac
    27721 gcggcaagga tgagtctgct ccggcactgg agccggaacg tcgcattaat aacaacatcg
    27781 tgctgaaaaa attacgcatc gcgttttcgc tgaaaaccga tgacattctg gcgatcctca
    27841 ccgaacagca gttccgcgtt tcgatgccgg aaattacagc gatgatgcgc gcaccggatc
    27901 ataaaaactt ccgcgaatgc ggcgatcaat ttttacgtta ttttctgcgt ggactggcag
    27961 cgcgccagca tgtgaagaaa agctaagacg ggtatggcgg ccatgcaaaa acatggccgc
    28021 cgacagatta tttcacttct ttaaaaccag cggctttcat caccagttcc atttgcgcca
    28081 tagtgatacc ttttttggca tcttcagcag aaacgttgat tcctgaaata ccctgcaggg
    28141 ctttgaaatc cactttttcc atatcgatag tcacgttttc ctgcgcgtaa gtatcggtat
    28201 aggttaattt ttcttcaaca cccgcgatgt ttttgtattt ggcgcttaac ggctcaagtg
    28261 tcctggcagc gtcttctttg gtggttgcac caatggaggc aaattgaatt ttggtttcag
    28321 aagattgctt aagcaccttg tcacctttgt agacataggt aatggcaatt tcagtaccgt
    28381 tcagattggc gctgaatttt ttcgattctt ctttgtcacc gcagccagca agagagaaaa
    28441 ccagaacaga tgcaacaacg agggaaaaca gcttattgaa agccttcatg taaaactcca
    28501 ttttatttaa tcaaggaact ggtgactctc accaggggct atataggata tgcctaatac
    28561 cgtggcgtga gcagtccgga actggagtag aactcttagt aaaaagcact atttcatcct
    28621 tgttgctgaa gcatggggaa taattgttcg cacagcaaaa caccgttatt cattgcttct
    28681 acccgtgcct cgctttctgt attacgaaat tgtcccaaca catgtgccag ccgataaaaa
    28741 cccaccgcgg tgaggtcatt cgccagcaac tctgcctgac caatagcact atgttcctga
    28801 tagcgccagc cgttatggag cagttgaata agtaacgcct ggcagcgcat cagcaactga
    28861 tgagcagtag acggcacagg cagaacgctg gcagaaggta gtgatgccac cacaggcgca
    28921 gtttctgcgt ccagcgccca ggcacgagtc tttgtcatca ttacctgtgg ttccagtgtc
    28981 aattgcccct ctacaaagct gacaaagcca gaaaccagac acacggggtc gtctgtttgt
    29041 tgcaaaagcg ccgccatgcg ttcaacggca taaggcgcgc tggctgaggc tggcagtgat
    29101 aacgtcagca gattatcttc accttcgccg ctgattacct gcgcatccag cgtctggcgg
    29161 ctgctatccc aaccgagcga aatacactca gcgaccggca gaataaataa gttatcgacc
    29221 tgattaagag gccgtatgca ggcgggggga cgctggcgta aatattcccg caaagccaca
    29281 atgcccggct ggcgtaacgg cgcgctcaac atttgccagg catcaggcga cagcggcaca
    29341 acgctgctta agcggttgcg ggtagctaac agcagctcgc catcggcact gcgttttgct
    29401 gcttgtgaaa caatttgccc gcccgccagt gcgccagcct gaaaactaaa cagccgacgc
    29461 gtcgctgccg gtgagttttc ctgttcgctt ctcggccaac tacgcgaaag atgcaaaata
    29521 ctgccggtgt cgggatcggt aaaccagatg cgtaaaccat aatgctcaat atcctgccag
    29581 caacgcatac ctaaagacac cagccgcaga tgatcaagct ttgcttctcc ggcaatgcca
    29641 gagccaacga ccgtgcgcca cggcatgggc ggtacttcac cgacactgtc acgtcgcgct
    29701 atctcttgtg cactgttcaa gcgactggtt aacgccgcaa gctggcacaa gcattttccg
    29761 gcatgatagt ggctggcgcg ggcgtggaag gcatcaacgc tggcgcgcag ttgccgtagc
    29821 gattcactca cccatcgcca gttgcagcgt tccgccgcct gctgtgcgcg gctgaaagcg
    29881 gcctcgtaat ggattggcgg ctggctgatg ccgcccagcc ataatgcctg acttaattgc
    29941 tgaacatatt gacgacacgc gttgccctct tcgctggcaa acggatcgtc agatgatgtg
    30001 acgtgttcgc tgcgcatctg ccagattaaa tgagtaaatt ccgcttgctg agttttggcc
    30061 tcgacgaagg cctgcacagc cagtacgaca tgttcgcaaa gtgtgccgtc aatacaatca
    30121 cagcgagcga aacgaatgct gctgcgggaa taaaaacgta catcgctcat cggtaagcgg
    30181 gcagagggaa tttcgcccgg cgtacagaac aactcaatgg tgatgccttt agcgaccagc
    30241 gcctgtgcgc gtttgcgggt ggcatcgggc agggtagcca gttcttccag ccagattgcc
    30301 ggatcccact cttcttcttt ttccgtaggc tgggtggtgg cgcaaagtcg ctgataactt
    30361 aaaaccagca tcacgcgatg gcggcacatg ccgctggccc cgcagctgca ctgagcctct
    30421 ttcagtgcct ggccgttcgc cagctgggtg cggacaccgt cactgaaggt ggcgattaaa
    30481 gcgccgttct catgactgat ttccgggacg ttgccatttt ccagttcttt aaggctgcgc
    30541 ttaacaaaac cggcattgct taacgccgtc agtgcctgtg gtgtcagttc taataattcc
    30601 ggacgtagtg aattcatgac tgaagattct ccgcaagcca ggtcgccagt tcgcccggcg
    30661 tcatggcggc tatttgtgca ccgacattaa ccagtgcctg ggccatatcg cggtcatagc
    30721 aaggtgttgc ggtgctatcg agcgccgcca gtcccagcac tttgatgccg ctctggacac
    30781 actttttcac ctgatgcgtc agcaatgatg atgaaccacc ttcatagaaa tcgctcacga
    30841 ggataatgac actttttgca ggttgttcaa taagttgccg accatactcc acggcactgg
    30901 cgatattggt cccgccgccc agttgtactt tcattaataa ctctaccgga tcggcaacgt
    30961 ctgccgtgag atcaaccacg ctggtgtcaa acgccaccag atgggtacga atgccgggta
    31021 actgccataa acaggccgcc atcaccgctg agtggatcac cgagtcaacc atcgatccgc
    31081 tttgatcaac cagtaagacc agttgccatt gatcgctatg gcgcttaatg cggctgttaa
    31141 agcgggggga ttcgatatac aacttgccgt gttgcggatg ccagtgttgc aggttggcac
    31201 gcagcgtact tttgaaatcg aaattacgcg ccagtggaat aaatgagcga cggcggcgat
    31261 cgcggacacc agaaaaagcc tgacgaactt cctttgccag tcgcgccata atttcttcaa
    31321 caacctggcg cactattcgg cgggcggcag ccagcacttc gggattcatc agatgtttgg
    31381 tgtgcaaaac ggcgcgtagc aggctttcag aaggctgcat acgttccagc acgtcgagat
    31441 tcgtcaccac atcttcaatg ccgtagcgca gtacggcatc gctttccagt cgctcgatca
    31501 cccgttgcgg aaacagcgtg tgaatactgt tgatccactc aggggtggtg agatttgagt
    31561 cacctaatcc accggagcgt tcaccacgct ggagccgttc aggatcgcgc ccatacagcc
    31621 actccagcgc gtggtctatc tgccgggcgt tgtcatccag cccacaaagc gtcgtttctg
    31681 ccgcttcgcc aagaattaat cgccagcgtt gtagctcacg ggtggtcaga agatcgttca
    31741 gttcagacat ggaaaactcc ccagtgttgc agcgatgcca gtgcctgctg ttcgagttgt
    31801 tgatgatgtg caatgtcttg tggtggacaa tgcaacggca tttgcagtgc cgaaacggga
    31861 agttgcacca gttgataatg ctcaagcacc tgatgcgcca gcgtcccgcg ttctcgtggt
    31921 ggtagccagg ccatcgccgc gcgtaaatcg ggcagggcat tgataaaatc ggcatcactc
    31981 agttgattta aatgactgct gaaaccggcg ataaataccg gctgacaggc cagttgatga
    32041 cgggctaatg ccagcaaacc gtgcagcgcc tcgccggatt gtgtcgggga taattgcacc
    32101 agcattgtca gcgccgcttc ggcactggcg ttgggatgtt ccagacgcat cagtgcgcca
    32161 agtgccgcgc cgcgatccag cgccggggca tgaatcgcct gactgcgtcg ctccagtaag
    32221 gctaccgccg cagaaagaga aacgccgggt aaattaacgc cgctatgtag atcgcgcagg
    32281 atatggcaaa gcgcttgcca gctatgcagg tgagcgtgaa actccttttc atccggtctg
    32341 ccgttggatt cacatagcca cagcgtgcga tcgatggccg cgcataatgt cgtttgtaat
    32401 atctgcgcgc cctgcatacc gctaatttca tccagccgcc ataaggcata tagcacttcc
    32461 agcgcctggc ccatttcggc aaactgattt tcctgggcga ttaataatgt gagttgctcc
    32521 agtaattgtt gattgaagga cgccagaccc gctaacgccg cctggctaag acatgtggtg
    32581 atactgccga ttccacccgc gtccagcata tcggcttcta atttattgcg tgcggcttcc
    32641 tggagtgtgg caccaaagca ggcggcctca attaatgccg catgttggct aagcgggcgg
    32701 gttaatttcc agcgttcttc accgttgcct gcaagtgtca gtgtacttcc ctgctggcgc
    32761 acaacccctg ggatctccag tattaccagc cgatgtaaca cctgactttg cgccagtcca
    32821 tcgggggtaa agcgatttaa agtaagctcc gccggaaaag agattcctgt acgttccagt
    32881 tcgcaggtga catcttttgg cagaggcggc tgtggcgtag agggggcaag tttaccgaat
    32941 ccgtcacccg ctaatgtgtc tatcaacgtt agcagaatcg gatcggtatc gggatgaata
    33001 acgccgcgat agctccacgg caacggcgca ttcagggctt ctttaatcag cgagcctgct
    33061 atggcatcca gccagtcagt gcgtaacggt agtgtatgac cacgcaactg tgccagtgcc
    33121 atcgcatgca gatgagcggc agccatatcc gctgttgaag caggcaagtg gtgctggcgc
    33181 aaacgggtaa gaaccgtttt cagcagttgt tcacctgcct gctgcaagcc ccaccgccag
    33241 caccagtttt gccagaccgg ggcaggcata ccggaaaggt atcctgccag cacatcaagg
    33301 cgcttttcac tgtagggggt gagataacaa cctgtaacgg catctgccag cgagggcaat
    33361 tccggcgtgt taatttcctg cgggcattcg cgccacattt ttgccagtgc agaagcgtgc
    33421 cagccaccgc agacgactaa cacgtcgcca ttgttttgct gcatcgccca accaatccag
    33481 cgggccataa agtcttcgcg ctgacgattg agagcatctc cgggggaatc gccccgcagt
    33541 tgtgcaaaat agtgcgccag tgcagagggt aatgcagttt gctggctttc atcttcgaac
    33601 aagtgatccc acagggtgtc gctgttatcc atgcgggtgg cacgcagcag caaggtctgg
    33661 ctttcctctt gcgtatcagg cgaatcgtcc tcttcttcac tctgcgccca gcaaggcaaa
    33721 tcgatgaagt aagtttgtgc ctgaatacga cgagcggctt gtagcgcctg ccactccggc
    33781 gaaaattcag catatggcgt ccaggcacca cgcccggggg ctgcaccgtc ctgatactgg
    33841 caataactgt aaatagctac cggaagctgg tgggctaaaa acagttcgtc tacccggtca
    33901 ttaaaatcag ccgggccttc aatcaacacg tatcgtggcc gctggctttc gattaacgat
    33961 ttcaccagcc gggcgcaggc cggactatga tgccggatgc cgacaattaa cggctcgctc
    34021 atgcatcttt cctcacggca ggcggtggcg agcttgataa taagcctgcc agtgagcttc
    34081 tttatgtgtg gcgactcgct gttcgaagta acggcgcaga cgagcgcgat cttcttcgtt
    34141 atctttgacg atggttccgg caatacagtc caccagatcg gctggctcac ctgcgcgatt
    34201 cgctaaaaac caggcgcgga cgcccactgc gtgggccacg ttcacggctt cagcggtgga
    34261 cataattgcc gtcagggtat ccattgacgt tttcttttcg ccgttggcac gcagatcgcg
    34321 gaaggtgcgg accagtaact ccagtaccgc gtcaggcact ttatgcggaa taccgctatg
    34381 ggccagcaaa cgcgccgaag cactggcgac cagttccagc tcctgggcga aatccataat
    34441 cggaaagacg gtttcaaaat cgaagcgacg ctttagcgcg gcactcatct cattgacgcc
    34501 gcgatcgcgg gtattggcag tggcaataat attaaagcct tctcgcgcat agagctgact
    34561 ggcttcaccg gtgagttccg gcaccgtcat taccctgtca gaaagcatac ctaacagaca
    34621 gtcttgtact tccagcggtg tacgcgttat ctcttcaaaa cggacaattt tgccgtcgcg
    34681 cattccctgg taaagcgggg cagggaccag cgcttccgtt gacggaccat ggttgataag
    34741 cagggcgtag ttccagccgt acttgatttg atcttcggtg gtagatgcgc cgccctggat
    34801 ggttaacccg gcatcgccgc taatagcggt tgccagtaat tcagacagga gcgattttgc
    34861 ggtgccaggt tcccccacca acatcaaacc gcgcccggta gcgagagtga ctaacatgcg
    34921 ttcgacagcg gagggactga tcacgacttt acggctaata ttttgcgcgc tatcgccaag
    34981 gataaaagcg cgggccgcag gcaaacttaa ctgccaaccg ggcgggcaag gtgcgtgatc
    35041 attttgtttt aattttgcca gttcatcggc gtataacaca gcggcaggcg gacgctgcag
    35101 atgattattc tgcggtgaca tatattcatc cctgaaatgt attaatgaaa taaacgatat
    35161 tttacgcgca ataaatgttt gccgcattag ttgtttgagc gaaaaatatc tggaaagcag
    35221 atcacagtaa ataaaaaaac ctgctggtga ggcaggttct tttatttact acaatgacgc
    35281 taacttgctg attgtgcaac atttcgtacc aacgaataaa ttataaccat atccacactc
    35341 cattgcaatt cagtgaatga gatttataat cctgcccgtt gggaggttgt gccggatcgg
    35401 accaatttta agtcctggct gctggacggg tggtgccgca ggcggtgccc tgatccggag
    35461 agcaacgttt cgtaccaaca tacgtaaacc agtatcgaca ggcagtgtaa gggtaaggtg
    35521 ctggtgttgc gctccttaat aaggagagcg gatggacacg c
//
LOCUS       DAZFLD010000016        35119 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.016, whole genome shotgun sequence.
ACCESSION   DAZFLD010000016 DAZFLD010000000
VERSION     DAZFLD010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 35119)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35119)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35119
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.016"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            1..162
                     /locus_tag="ACHKBL_000748"
     CDS             1..162
                     /locus_tag="ACHKBL_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5817663.1"
                     /translation="MITLVILFCRRPHCRKISVQQYYQKRVIFYPTTLQRTAFKKCLF
                     LMRDIIISS"
     gene            123..242
                     /locus_tag="ACHKBL_000749"
     CDS             123..242
                     /locus_tag="ACHKBL_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HFO5817664.1"
                     /translation="MPLSYAGYHYFIMMSLVSGEHNTLALPIQEQSPGLVPAG"
     gene            277..621
                     /locus_tag="ACHKBL_000750"
                     /pseudo
     CDS             277..621
                     /locus_tag="ACHKBL_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(986..1252)
                     /gene="minE"
                     /locus_tag="ACHKBL_000751"
     CDS             complement(986..1252)
                     /gene="minE"
                     /locus_tag="ACHKBL_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032955 - regulation of division septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HFO5817665.1"
                     /translation="MALLDFFLSRKKNTANIAKERLQIIVAERRRSDAEPHYLPQLRK
                     DILEVICKYVQIDPEMVTVQLEQKDGDISILELNVTLPEAEELK"
     gene            complement(1256..2068)
                     /gene="minD"
                     /locus_tag="ACHKBL_000752"
     CDS             complement(1256..2068)
                     /gene="minD"
                     /locus_tag="ACHKBL_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HFO5817666.1"
                     /translation="MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLR
                     NLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRTENLYILPASQTRDKDALTREGV
                     AKVLDDLKAMDFEFIVCDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGIL
                     ASKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVL
                     RASNQGEPVILDINADAGKAYADTVERLLGEERPFRFIEEEKKGFLKRLFGG"
     gene            complement(2092..2787)
                     /gene="minC"
                     /locus_tag="ACHKBL_000753"
     CDS             complement(2092..2787)
                     /gene="minC"
                     /locus_tag="ACHKBL_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004857 - enzyme inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA];
                     GO_process: GO:1901891 - regulation of cell septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HFO5817667.1"
                     /translation="MSNTPIELKGSSFTLSVVHLHEAEPKVIHQALEDKIAQAPAFLK
                     HAPVVLNVSALEDPVNWSAMHKAVSATGLRVIGVSGCKDAQLKAEIEKMGLPILTEGK
                     EKAPRPAPAPQAPAQNTTPVTKTRLIDTPVRSGQRIYAPQCDLIVTSHVSAGAELIAD
                     GNIHVYGMMRGRALAGASGDRETQIFCTNLMAELVSIAGEYWLSDQIPAEFYGKAARL
                     QLVENALTVQPLN"
     gene            3306..3674
                     /gene="ycgJ"
                     /locus_tag="ACHKBL_000754"
     CDS             3306..3674
                     /gene="ycgJ"
                     /locus_tag="ACHKBL_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgJ family protein"
                     /protein_id="HFO5817668.1"
                     /translation="MNMMRIFYIGLSGVGMMFSSMASGHDAGGLQSPACGVVCDPYIC
                     VNSDGISPELTRKYLGEKAAENLQSLQGYDPSEFTFANGVFCDVKEKLCRDDRYFGVN
                     GKRSGKINQTTTKMLFMCRE"
     gene            complement(3777..4178)
                     /gene="pliG"
                     /locus_tag="ACHKBL_000755"
     CDS             complement(3777..4178)
                     /gene="pliG"
                     /locus_tag="ACHKBL_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="g-type lysoyme inhibitor PliG"
                     /protein_id="HFO5817669.1"
                     /translation="MKIKGISKAVLLLALLTSTSFAAGKNVNVEFRKGHSSAQYSGEI
                     KGYDYDTYTFYAKKGQKVHVSISNEGADTYLFGPGIDDSVDLSRYSPELDSHGQYSLP
                     ASGKYELRVLQTRNDARKNKTKKYNVDIQIK"
     gene            4420..4713
                     /locus_tag="ACHKBL_000756"
     CDS             4420..4713
                     /locus_tag="ACHKBL_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgL domain-containing protein"
                     /protein_id="HFO5817670.1"
                     /translation="MFCVIYRSSKRDQTYLYVEKKDDFSRVPEELMKGFGQPQLAMIL
                     PLDGRKKLVNADIEKVKQALTEQGYYLQLPPPPEDLLKQHLSVMGQKTDDTNK"
     gene            4785..5444
                     /gene="ycgM"
                     /locus_tag="ACHKBL_000757"
     CDS             4785..5444
                     /gene="ycgM"
                     /locus_tag="ACHKBL_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HFO5817671.1"
                     /translation="MYQHHNWQGALLDYPVSKVVCVGSNYAKHIKEMGSAVPEEPVLF
                     IKPETALCDLRQPLAIPSDFGSVHHEVELAVLIGATLRQATEEHVRKAIAGYGVALDL
                     TLRDVQGKMKKAGQPWEKAKAFDNSCPLSGFIPAAEFTGDPQNTTLGLSVNGEQRQQG
                     TTADMIHKIVPLIAYMSKFFTLKAGDVVLTGTPDGVGPLQSGDELTVTFDGHSLTTRV
                     L"
     gene            5536..5982
                     /gene="ycgN"
                     /locus_tag="ACHKBL_000758"
     CDS             5536..5982
                     /gene="ycgN"
                     /locus_tag="ACHKBL_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309703.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HFO5817672.1"
                     /translation="MSDVPFWQSKTLDEMSDAEWESLCDGCGQCCLHKLMDEDTDEIY
                     FTNVACRQLNIKTCQCRNYERRFEFEPDCIKLTRENLPTFEWLPMTCAYRLLAEGKDL
                     PAWHPLLTGSKAAMHGERISVRHIAVKESEVIDWQDHILNKPDWAQ"
     gene            complement(6189..6515)
                     /gene="hlyE"
                     /locus_tag="ACHKBL_000759"
                     /pseudo
     CDS             complement(6189..6515)
                     /gene="hlyE"
                     /locus_tag="ACHKBL_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415700.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin HlyE"
     gene            6776..7195
                     /gene="umuD"
                     /locus_tag="ACHKBL_000760"
     CDS             6776..7195
                     /gene="umuD"
                     /locus_tag="ACHKBL_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HFO5817673.1"
                     /translation="MLFIKPADLREIVTFPLFSDLVQCGFPSPAADYVEQRIDLNQLL
                     IQHPSATYFVKASGDSMIDGGISDGDLLIVDSAITASHGDIVIAAVDGEFTVKKLQLR
                     PTVQLIPMNSAYSPITISSEDTLDVFGVVIHVVKAMR"
     gene            7195..8463
                     /gene="umuC"
                     /locus_tag="ACHKBL_000761"
     CDS             7195..8463
                     /gene="umuC"
                     /locus_tag="ACHKBL_000761"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
                     /protein_id="HFO5817674.1"
                     /translation="MFALCDVNAFYASCETVFRPDLWGKPVVVLSNNDGCVIARNAEA
                     KALGVKMGDPWFKQKDLFRRCGVVCFSSNYELYADMSNRVMSTLEELSPRVEIYSIDE
                     AFCDLTGVHNCRDLTDFGREIRATVLQRTHLTVGVGIAQTKTLAKLANHAAKKWQRQT
                     GGVVDLSNLERQRKLMSALPVDEVWGIGRRISKKLDAMGIKTVLDLADSDIRFIRKHF
                     NVVLERTVRELRGEPCLQLEEFAPTKQEIICSRSFGERITDYPSMRQAICSYAARAAE
                     KLRSEHQYCRFISTFIKTSPFAFNEPYYGNSASVKLLTPTQDSRDIINAATRSLDAIW
                     QAGHRYQKAGVMLGDFFSQGVAQLNLFDDNAPRPGSEQLMAVMDTLNAKEGRGTLYFA
                     GQGIQQQWQMKRAMLSPCYTTRSSDLLRVK"
     gene            complement(8508..9038)
                     /gene="dsbB"
                     /locus_tag="ACHKBL_000762"
     CDS             complement(8508..9038)
                     /gene="dsbB"
                     /locus_tag="ACHKBL_000762"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HFO5817675.1"
                     /translation="MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLC
                     IYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFA
                     TCDFMARFPEWLPLDKWVPQVFVASGDCAERQWEFLGLEMPQWLLGIFIAYLIVAVLV
                     VISQPFKAKKRDLFGR"
     gene            complement(9184..10725)
                     /gene="nhaB"
                     /locus_tag="ACHKBL_000763"
     CDS             complement(9184..10725)
                     /gene="nhaB"
                     /locus_tag="ACHKBL_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter NhaB"
                     /protein_id="HFO5817676.1"
                     /translation="MEISWGRALWRNFLGQSPDWYKLALIIFLIVNPLIFLISPFVAG
                     WLLVAEFIFTLAMALKCYPLLPGGLLAIEAVFIGMTSAEHVREEVAANLEVLLLLMFM
                     VAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCVAAAFLSAFLDALTVVAVVISVAVG
                     FYGIYHRVASSRTEDTDLQDDSHIDKHYKVVLEQFRGFLRSLMMHAGVGTALGGVMTM
                     VGEPQNLIIAKAAGWHFGDFFLRMSPVTVPVLICGLLTCLLVEKLRWFGYGETLPEKV
                     REVLQQFDDQSRHQRTRQDKIRLIVQAIIGVWLVTALALHLAEVGLIGLSVIILATSL
                     TGVTDEHAIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIQFVLQASEHAQLSLF
                     YIFNGLLSSISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPN
                     GQAAFLFLLTSALAPLIRLSYGRMVWMALPYTLVLTLVGLLCVEFTLAPVTEWFMQMG
                     WIATL"
     gene            10947..11666
                     /gene="fadR"
                     /locus_tag="ACHKBL_000764"
     CDS             10947..11666
                     /gene="fadR"
                     /locus_tag="ACHKBL_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000062 - fatty-acyl-CoA binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0019217 - regulation of fatty acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HFO5817677.1"
                     /translation="MVIKAQSPAGFAEEYIIESIWNNRFPPGTILPAERELSELIGVT
                     RTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLL
                     SVRTNISTIFIRTAFRQHPDKAQEVLATANEVADHADAFAELDYNIFRGLAFASGNPI
                     YGLILNGMKGLYTRIGRHYFANPEARSLALGFYHKLSALCSEGAHDQVYETVRRYGHE
                     SGEIWHRMQKNLPGDLAIQGR"
     gene            complement(11718..13250)
                     /gene="ycgB"
                     /locus_tag="ACHKBL_000765"
     CDS             complement(11718..13250)
                     /gene="ycgB"
                     /locus_tag="ACHKBL_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HFO5817678.1"
                     /translation="MATIDSMNKDTTRLSDGPDWTFDLLDVYLAEIDRVAKLYRLDTY
                     PHQIEVITSEQMMDAYSSVGMPINYPHWSFGKKFIETERLYKHGQQGLAYEIVINSNP
                     CIAYLMEENTITMQALVMAHACYGHNSFFKNNYLFRSWTDASSIVDYLIFARKYITEC
                     EERYGVDEVERLLDSCHALMNYGVDRYKRPQKISLQEEKARQKSREEYLQSQVNMLWR
                     TLPKREEEKTVAEARRYPSEPQENLLYFMEKNAPLLESWQREILRIVRKVSQYFYPQK
                     QTQVMNEGWATFWHYTILNHLYDEGKVTERFMLEFLHSHTNVVFQPPYNSPWYSGINP
                     YALGFAMFQDIKRICQSPTEEDKYWFPDIAGSDWLETLHFAMRDFKDESFISQFLSPK
                     VMRDFRFFTVLDDDRHNYLEISAIHNEEGYREIRNRLSSQYNLSNLEPNIQIWNVDLR
                     GDRSLTLRYIPHNRAPLDRGRKEVLKHVHRLWGFDVMLEQQNEDGSVELLERCPPRMG
                     NL"
     gene            13580..14878
                     /gene="dadA"
                     /locus_tag="ACHKBL_000766"
     CDS             13580..14878
                     /gene="dadA"
                     /locus_tag="ACHKBL_000766"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HFO5817679.1"
                     /translation="MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANA
                     GQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYM
                     ENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDA
                     GVPYQLLESSRLAEVEPALAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKF
                     RFNTPVDQLLCDGEQIYGVKCGDEVIKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYS
                     LTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMV
                     VRDLYPRGGHVEQATFWTGLRPMTPDGTPVVGRTRFKNLWLNTGHGTLGWTMACGSGQ
                     LLSDLLSGRTPAIPYEDLSVARYSRGFTPSRPGHLHGAHS"
     gene            14888..15958
                     /gene="dadX"
                     /locus_tag="ACHKBL_000767"
     CDS             14888..15958
                     /gene="dadX"
                     /locus_tag="ACHKBL_000767"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309712.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolic alanine racemase DadX"
                     /protein_id="HFO5817680.1"
                     /translation="MTRPIQASLDLQALKQNLSIVRQAAPYARVWSVVKANAYGHGIE
                     RIWSALGATDGFALLNLEEAITLRERGWKGPILMLEGFFHAQDLEIYDQHRLTTCVHS
                     NWQLKALQNARLKAPLDIYLKVNSGMNRLGFQPDRVLTVWQQLRAMANVGEMTLMSHF
                     AEAEHPDGISSAMARIEQAAEGLECRRSLSNSAATLWHPEAHFDWVRPGIILYGASPS
                     GQWRDIANTGLRPVMTLSSEIIGVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGY
                     PRHAPTGTPVLVDGVRTMTVGTVSMDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAA
                     AAGTVGYELMCALALRVPVVTV"
     gene            complement(16015..17751)
                     /gene="cvrA"
                     /locus_tag="ACHKBL_000768"
     CDS             complement(16015..17751)
                     /gene="cvrA"
                     /locus_tag="ACHKBL_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006884 - cell volume homeostasis [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HFO5817681.1"
                     /translation="MDATTIISLFILGSILVTSSILLSSFSSRLGIPILVIFLAIGML
                     AGVDGVGGIPFDNYPFAYMVSNLALAIILLDGGMRTQASSFRVALGPALSLATLGVLI
                     TSGLTGMMAAWLFNLDLIEGLLIGAIVGSTDAAAVFSLLGGKGLNERVGSTLEIESGS
                     NDPMAVFLTITLIAMIQQHESSVSWMFVVDILQQFGLGIVIGLGGGYLLLQMINRIAL
                     PAGLYPLLALSGGILIFALTTALEGSGILAVYLCGFLLGNRPIRNRYGILQNFDGLAW
                     LAQIAMFLVLGLLVNPSDLLPIAIPALILSAWMIFFARPLSVFAGLLPFRGFNLRERV
                     FISWVGLRGAVPIILAVFPMMAGLENARLFFNVAFFVVLVSLLLQGTSLSWAAKKAKV
                     VVPPVGRPVSRVGLDIHPENPWEQFVYQLSADKWCVGAALRDLHMPKETRIAALFRDN
                     QLLHPTGSTRLREGDVLCVIGRERDLPALGKLFSQSPPVALDQRFFGDFILEASAKYA
                     DVALIYGLEDGREYRDKQQTLGEIVQQLLGAAPVVGDQVEFAGMIWTVAEKEDNEVLK
                     IGVRVAEEEAES"
     gene            complement(17846..18760)
                     /gene="ldcA"
                     /locus_tag="ACHKBL_000769"
     CDS             complement(17846..18760)
                     /gene="ldcA"
                     /locus_tag="ACHKBL_000769"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muramoyltetrapeptide carboxypeptidase"
                     /protein_id="HFO5817682.1"
                     /translation="MSLFHLIAPSGYCIKQHAALRGIQRLTDAGHQVNNVEVIARRCE
                     RFAGTETERLEDLNSLARLTTPNTIVLAVRGGYGASRLLADIDWQALVARQQHDPLLI
                     CGHSDFTAIQCGLLAHGNVITFSGPMLVANFGADELNAFTEHHFWLALRNETFTIEWQ
                     GEGPTCQTEGTLWGGNLAMLISLIGTPWMPKIENGILVLEDINEHPFRVERMLLQLYH
                     AGILPRQKAIILGSFSGSTPNDYDAGYNLESVYAFLRSRLSIPLITGLDFGHEQRTVT
                     LPLGAHAILNNTQEGTQLTISGHPVLKM"
     gene            18860..19471
                     /gene="emtA"
                     /locus_tag="ACHKBL_000770"
     CDS             18860..19471
                     /gene="emtA"
                     /locus_tag="ACHKBL_000770"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     EmtA"
                     /protein_id="HFO5817683.1"
                     /translation="MKLRWFAFLIVLLAGCSSKHDYTNPPWNAKVPVQRAMQWMPISQ
                     KAGAAWGVDPQLITAIIAIESGGNPNAVSKSNAIGLMQLKASTSGRDVYRRMGWSGEP
                     TTSELKNPERNISMGAAYLNILETGPLAGIEDPKVLQYALVVSYANGAGALLRTFSSD
                     RKKAISKINDLDADEFLDHVARNHPAPQAPRYIYKLEQALDAM"
     gene            complement(19473..20207)
                     /gene="ycgR"
                     /locus_tag="ACHKBL_000771"
     CDS             complement(19473..20207)
                     /gene="ycgR"
                     /locus_tag="ACHKBL_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HFO5817684.1"
                     /translation="MSHYHEQFLKQNPLAVLGVLRDLHKAAIPLRISWNGGQLISKIL
                     AITPDKLVLDFGSQAEDNNAVLKAQHITITAETQGAKVEFTVEQLQQSEYLQLPAFIT
                     VPPPTLWFVQRRRYFRISAPLHPPYFCQTKLADNSTLRFRLYDLSLGGMGALLETAKP
                     AGLHEGMRFAQIEVNMGQWGVFHFDAQLISISERKVIDGKNETITTPRLSFRFLNVSP
                     TVERQLQRIIFSLEREAREKADKVRD"
     gene            20408..20662
                     /gene="ymgE"
                     /locus_tag="ACHKBL_000772"
     CDS             20408..20662
                     /gene="ymgE"
                     /locus_tag="ACHKBL_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFO5817685.1"
                     /translation="MGIIAWIIFGLIAGIIAKLIMPGRDGGGFFLTCILGIVGAVVGG
                     WLATMFGIGGSISGFNLHSFLVAVVGAILVLGVFRLLRRE"
     gene            complement(20712..22682)
                     /locus_tag="ACHKBL_000773"
     CDS             complement(20712..22682)
                     /locus_tag="ACHKBL_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HFO5817686.1"
                     /translation="MRLKKRYLCTALSLAFTQQALAAQESDTLTVWSSPVSSTTTTVL
                     DHPTMKALDKQNVAQALSVVPGVVLQKSGSRNEEQVKVRGFDSRQVPVYFDGVPIYVP
                     YDGNLDLARILTNNLGAVEVSKGYSSLLQGPNQMGGAINITTQKPTKPLEANLGYRQG
                     WSRSQDNAYDMHASFAASSDLGYLQVSGSQLKQDFLGLPHGVNNDIAGKHGKMINSSA
                     DDKRGIVKLGFTPRENDEYTLTYIKQDGEKDNPPYSGNSGQKSRYWQWPEYDKESFYY
                     QGTTQLNDRFTLKSRLYRDTFENTLMMYNSLADLKNKKGSYSHYSDYSDGAGLQLAAD
                     VRENDLLSFAVNWKDDVHREKGAPHAAYDRYEDRTWSLASEYQWAAADNVDVVAGISY
                     DWRDSVEAKKHEKDGSITHYDDNNQSAFNWQVMGKYHFANEDTLALSYYDRTRFPTLK
                     ERYTTSKPAYNQIAIVNPQLKPERARGVDLTWNGAFTHDWGFEVSVYYNRVSDAILSH
                     NIDADTIQNQNSGTVDYSGLDAGIKGKISNILDVGLSYALIHADAKRKDIGKITDLPT
                     QTMTAWMTLKPWEPLSVTLSEEARSSSYSNSDGSQKAAGFAVTHIRADYTLGHGFSVN
                     ASVNNLFDTKYAYSEGFIEEGRNFWAGIEYTF"
     gene            complement(22708..23562)
                     /gene="modD"
                     /locus_tag="ACHKBL_000774"
     CDS             complement(22708..23562)
                     /gene="modD"
                     /locus_tag="ACHKBL_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ModD protein"
                     /protein_id="HFO5817687.1"
                     /translation="MIFLSQAQIDALLLEDIQGGDLTTRALNIGHQHGYIEFFHRQGG
                     CVSGISVACKMLTTLGLTIDDAVSDGSQANAGQRLIRAQGNAAALHQGWKAVQNVLEW
                     SCGVSDYLAQMLALLRERYPDGNIACTRKAIPGTRLLASQAILAAGGLIHRAGCAETI
                     LLFANHRHFLHDNQDWSGAINQLRRHAPEKKIVVEADTPKEAIAALRAQPDVLQLDKF
                     NPQQATEIAQIAPSLAPHCTLALTGGINLTTLKNYLDCGIRLFITSAPYYATPADIKV
                     SLQPAASI"
     gene            complement(23559..24371)
                     /locus_tag="ACHKBL_000775"
     CDS             complement(23559..24371)
                     /locus_tag="ACHKBL_000775"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000903984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFO5817688.1"
                     /translation="MLIDDIDFADLYLQQLRLAHRTEKTPDHWDQRAEKMAENCASPT
                     DSYLQQLTSKIDLQGAQTLFDMGCGPGTVSLALADKLTTIYGVDYSQGMLNVAARRAA
                     ALKADNVHWIQRAWEEDWSDLPRCDIAVASRSTLVADMRQAMSKLNNQARLRVYTTHL
                     VSTSFVSPAIQRAAGREVVELPNYIFALNVLYQMGIYAHVDFIRGQNCQQDNSTWERF
                     EQNVSWSLGALNDDERERLYRWYQQQDARALAPASRDWALIWWDSVPQEALR"
     gene            complement(24381..25139)
                     /locus_tag="ACHKBL_000776"
     CDS             complement(24381..25139)
                     /locus_tag="ACHKBL_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO5817689.1"
                     /translation="MTLLAVRHASLGYSRHPVLRDVSFTLSQGTICCLLGANGCGKTT
                     LMRSILGVIPLLKGEILLDSLPVQTLSHRQRAQAIAWVPQAHDGIFAFSVLDMVLMGQ
                     APTIGAFSVPGKQERLKATEQLEKLGILHLATRRWNTLSGGERQLALIARALVQQPRL
                     LLLDEPASSLDFGHQIQLLDTLAQLKNNGMTMLMSTHHPLHANAIADSIIQVEPDGRV
                     TQGLPTEQLTTKKLAALYRVSADQIHHHLSAIKH"
     gene            complement(25136..26116)
                     /locus_tag="ACHKBL_000777"
     CDS             complement(25136..26116)
                     /locus_tag="ACHKBL_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFO5817690.1"
                     /translation="MRTVNGCILLLAAISITFAAVSGAYHLDMQQLLALILRQENVTV
                     QEQIVFWQIRVPRILAALFLGAALAGAGTTYQGMFRNPLVSPDILGVSAGAGLGACAA
                     ILWGLSIVYIQLYAFCGGLMVVAGVWLITRRVTRHDPILTLVLVGIALGTLCGAGISL
                     IKTLADPYTQLPSITFWLLGGLSTVTLRDLCYAAPIILIGSLPLFFLRWRMNLLTLSD
                     DEARSLGLNVTRLRFGLIVCATLITASTVAIAGIIGWVGLVVPHIARLLTGHNHQQLL
                     PMAMCTGAILLLLTDTLARSIGTTEIPLGILTAFVGAPFFLFLLLRGGRQ"
     gene            complement(26116..27138)
                     /locus_tag="ACHKBL_000778"
     CDS             complement(26116..27138)
                     /locus_tag="ACHKBL_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter substrate-binding protein"
                     /protein_id="HFO5817691.1"
                     /translation="MSLTRRRFTQILASTLFLHHLPSFAQSVKFWASLTLPEAQNITR
                     IVSAGAPADLLLLAVAPEKMVGFSSFDFARQALIPLPEHIRQFPRLGRLAGRASTLSL
                     EGLMALHPDLVVDCGNTDETWISQARQVSEQTQIPWLLLNGKLEQSAEQLTTLGKTLG
                     EEHRAAEQANLASRFVGEAQAFATSPAANLSFYAARGPRGLETGLQGSLHTEAAELLG
                     LHNVAQIADRHGLTQVSMENLLRWQPDIILVQEAVTADFIRRDPLWQGVKAVAEQRIL
                     FLSGLPFGWLDAPPGINRLLGLRRLHAWLDPAINRQFKSDMQHYAQLFWHCPLSDADY
                     QKLVAS"
     gene            complement(27475..29172)
                     /gene="treA"
                     /locus_tag="ACHKBL_000779"
     CDS             complement(27475..29172)
                     /gene="treA"
                     /locus_tag="ACHKBL_000779"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000841732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HFO5817692.1"
                     /translation="MKSPAASRPLKMALIPACIFLCFAALSVQAEETPVTPQPPDILL
                     GPLFNDVQNAKLFPDQKTFADAVPNSDPLMILADYRMQQNQSGFDLRHFVNVNFTLPK
                     EGEKYVPPEGQSLREHIDGLWPVLTRSTENTEKWDSLLPLPEPYVVPGGRFREVYYWD
                     SYFTMLGLAESGHWDKVADMVANFAHEIDTYGHIPNGNRSYYLSRSQPPFFALMVELL
                     AQHEGDAALKQYLPQMQKEYAYWMDGVENLQAGQQEKRVVKLQDGTLLNRYWDDRDTP
                     RPESWVEDIATAKSNPNRPATEIYRDLRSAAASGWDFSSRWMDNPQQLNTLRTTSIVP
                     VDLNSLMFKMEKILARASKAAGDNAMANQYETLANARQKGIEKYLWNDQQGWYADYDL
                     KSHKVRNQLTAAALFPLYVNAAAKDRASKMATATKTHLLQPGGLNTTSVKSGQQWDAP
                     NGWAPLQWVATEGLQNYGQKEVAMDISWHFLTNVQHTYDREKKLVEKYDVSTTGTGGG
                     GGEYPLQDGFGWTNGVTLKMLDLICPKEQPCDNVPATRPLSESTTQPVKQNEAEPTP"
     gene            complement(29493..30911)
                     /gene="dhaM"
                     /locus_tag="ACHKBL_000780"
     CDS             complement(29493..30911)
                     /gene="dhaM"
                     /locus_tag="ACHKBL_000780"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415716.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0019563 - glycerol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase phosphoryl donor subunit
                     DhaM"
                     /protein_id="HFO5817693.1"
                     /translation="MVNLVIVSHSSRLGEGVGELARQMLMSDNCKIAIAAGIDDPQNP
                     IGTDAVKVMEAIESVADTDHVLVMMDIGSALLSAETALELLAPEIAAKVRLCAAPLVE
                     GTLAATVSAASGAEIDKVIFDAMHALEAKREQLGLPSSNTEISDTCPSYDEEARSLAV
                     VIKNRNGLHVRPASRLVYTLSTFNADMLLEKNGKCVTPESINQIALLQVRYNDTLRLI
                     AKGPEAEEALIAFRQLAEDNFGETEEVAPPILRPVPPVSGKAFYYQPVLCTVQAKSTL
                     TVDEEQERLRQAIDFTLLDLMTLTAKAEASGLDDIAAIFSGHHTLLDDPELLAAASEL
                     LQHEHCTAEYAWQQVLKELSQQYQQLDDEYLQARYIDVDDLLHRTLVHLTQTKEELPQ
                     FNSPTILLAENIYPSTILQLDPAVVKGICLSAGSPLSHSALIARELGIGWICQQGEKL
                     YAIQPEETLTLDVKTQRFSRQG"
     gene            complement(30922..31554)
                     /gene="dhaL"
                     /locus_tag="ACHKBL_000781"
     CDS             complement(30922..31554)
                     /gene="dhaL"
                     /locus_tag="ACHKBL_000781"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HFO5817694.1"
                     /translation="MSLSRTQIVNWLTRCGDIFSTESEYLTGLDREIGDADHGLNMNR
                     GFSKVVEKLPAIADKDIGFILKNTGMTLLSSVGGASGPLFGTFFIRAAQATQARQSLT
                     LEELYQMFRDGADGVISRGKAEPGDKTMCDVWVPVVESLRQSSEQNLSIPAALEAASS
                     VAESAAQSTITMQARKGRASYLGERSIGHQDPGATSVMFMMQMLALAAKE"
     gene            complement(31565..32635)
                     /gene="dhaK"
                     /locus_tag="ACHKBL_000782"
     CDS             complement(31565..32635)
                     /gene="dhaK"
                     /locus_tag="ACHKBL_000782"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415718.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HFO5817695.1"
                     /translation="MKKLINDVQDVLDEQLAGLAKAHPSLTLHQDPVYVTRSDAPVAG
                     KVALLSGGGSGHEPMHCGYIGQGMLSGACPGEIFTSPTPDKIFECAMQIDGGEGVLLI
                     IKNYTGDILNFETATELLHDSGVKVTTVVIDDDVAVKDSLYTAGRRGVANTVLIEKLV
                     GAAAERGDSLDACAELGRKLNNQGHSIGIALGACTVPAAGKPSFTLADNEMEFGVGIH
                     GEPGIDRRPFSSLDQTVDEMFDTLLENGSYHRTLRFWDYQQGSWQEESQTKQPLQSGD
                     RVIALVNNLGATPLSELYGVYNRLTTRCQQAGLTIERNLIGAYCTSLDMTGFSITLLK
                     VDDETLALWDAPVHTPALNWGK"
     gene            32863..34782
                     /gene="dhaR"
                     /locus_tag="ACHKBL_000783"
     CDS             32863..34782
                     /gene="dhaR"
                     /locus_tag="ACHKBL_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001467850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase operon transcriptional
                     regulator DhaR"
                     /protein_id="HFO5817696.1"
                     /translation="MSSAFNNDGRDISPLIATSWERCIKLMKRDTWNVPHQAQGVTFA
                     SIYRRKKAMLTLGQAALEDAWEYMAPRACALLILDETACILSRNGDPQTLQQLSALGF
                     NDGTYCAEGIIGTCALSLAAISGQAVKTMADQHFKQALWNWAFCATPLFDSKGRLTGT
                     IALACPVEQTTAADLPLTLAIAREVGNLLLTDSLLAETNRHLNQLNALLESMDDGVIS
                     WDEQGNLQFINAQAARVLRLDATASQGRAITELLTLPAVLQQAIKQAHPLKHVEATFE
                     SQHQFIDAVITLKPIIETQGTSFILLLHPVEQMRQLMTSQLGKVSHTFAHMPQEDPQT
                     RRLIHFGRQAARSSFPVLLCGEEGVGKALLSQAIHNESERAAGPYIAVNCELYGDAAL
                     AEEFIGGDRTDNENGRLSRLELAHGGTLFLEKIEYLAVELQSALLQVIKQGVITRLDA
                     RRLIPIDVKVIATTTADLAMLVEQNRFSRQLYYALHAFEITIPPLRMRRGSIPALVNN
                     KLHSLEKRFSTRLKIDDDALARLVSCAWPGNDFELYSVIENLALSSDNGRIRVSDLPE
                     HLFTEQATDDVSATRLSTSLSFAEVEKEAIINAAQVTGGRIQEMSALLGIGRTTLWRK
                     MKQHGIDAGQFKRRG"
     gene            complement(34819..>35119)
                     /locus_tag="ACHKBL_000784"
     CDS             complement(34819..>35119)
                     /locus_tag="ACHKBL_000784"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5817697.1"
                     /translation="ACYAASFNSELSKIIIRLLAILTMCLLCDNNHSALTVQEFVMKK
                     TFTFSLLAIVFASLLSACVPHHHHRHSDGPRGPAPSGKPMPPSNGGPNHGSHGRY"
BASE COUNT         8725 a         8628 c         9064 g         8702 t
ORIGIN      
        1 atgattacat tagtcatttt attttgtcga cggcctcatt gtcgaaaaat aagcgtacaa
       61 cagtattatc agaaaagagt gattttttat ccaactacac ttcagcgcac tgcgtttaaa
      121 aaatgcctct ttcttatgcg ggatatcatt atttcatcat gatgtctttg gtgagcggtg
      181 aacacaatac acttgcgctg cctattcagg agcaatcccc tggtctggtg cctgcgggat
      241 gatgttgcag cagagctaag gtcacttaga cgctactatc atttatccaa tggcatgggg
      301 tcgaaatcag tcgatacccg cagtatatat cgtgaacttg gtgcaacgct gagttacaac
      361 atgcgtctgg ggaacggcat ggaagttgag ccgtggctga aggcggctgt gcgcaaagaa
      421 tttgtcgatg ataaccgggt gaaagtgaat aatgacggta attttgtcaa tgatttgtcg
      481 ggcagacgtg gaatatatca ggcaggtatt aaagcctcat tcagcagttc gttaagcggg
      541 catctcgggg tggggtatag ccatggtgcc ggtgtggaat ccccatggaa cgcggtggct
      601 ggtgcgaact ggtcgttctg accatcaacg attaaactgc gcttcgcgca gttttcgttt
      661 accgggtgct aaaagggaaa attctgcggc aaaaaaagcc cgccagttac ggcgggaaac
      721 ctcatcctat gggagaacaa tgaataatga aattgcgggg ttatcatctc ccagtatacc
      781 catactaaca ataaggttat ttactcagcc aggtataaac aatttgttct gtgtgggggg
      841 aacggcatta agatgtaaag ggggggaagc aatgagtagc atcagcaaga atactcgccg
      901 ctcttatcaa acgtggatga gggggatgaa aaactcaagg cagagatgac tctgccttga
      961 agataaatgc gcttttacag cgggcttatt tcagctcttc tgcttccggt aaggtcacgt
     1021 tcagctcaag aatagaaata tcgccatctt tttgctcaag ctgtacggtt accatctcag
     1081 gatcaatttg tacgtattta caaatgacct caagaatatc tttacgcaac tgcggcagat
     1141 aatgcggttc tgcatcactg cgacggcgtt cagcaacaat aatctgcagc cgttcttttg
     1201 caatgttggc cgtgtttttc ttccgcgata gaaagaaatc gagtaatgcc ataacttatc
     1261 ctccgaacaa gcgtttgagg aagcctttct tctcttcttc aatgaagcgg aaaggacgtt
     1321 cttctcccaa cagacgttct acggtatctg cgtaggcttt acccgcatcg gcgttaatgt
     1381 cgagaatgac cggttcaccc tggttagagg cgcgcaatac tgattgatcc tcagggatca
     1441 cgccgacgag tttgatgcgc aggatctcca gcacatcttc catgctcagc atgtcacctc
     1501 tgcttacgcg gcctgggtta tagcgcgtta acagcaggtg ctctttaata ggctcttcgc
     1561 cattttctgc gcggcgtgat ttcgacgcca gaatgcctaa aatacggtca gagtcgcgta
     1621 ctgaggagac ttccgggttg gtggtaataa tggcttcgtc tgcaaaatag agtgccatta
     1681 gcgcaccggt ctcaatccct gccggggagt cgcaaacaat aaattcaaag tccatcgctt
     1741 tcagatcatc aagaactttg gcgacccctt cacgggtgag ggcatcttta tcgcgagttt
     1801 gcgatgccgg aagaatatag agattttcag tacgcttatc tttaattaac gcctgattta
     1861 gcgttgcatc gccctgaatg acgttgacga aatcgtaaac gacccgacgt tcacaaccca
     1921 taatcaggtc gagattacgc aggccgatat caaaatctat cacgacagtt ttctttccct
     1981 tctgggccaa accagtggcg atggccgcgc tggaggttgt cttaccaaca ccccctttgc
     2041 ccgaagtaac aacaataatg cgtgccatag aaattccttg ttaaaaaggg atcaatttaa
     2101 cggttgaacg gtcaaagcgt tttcgactaa ctgcagtcgc gccgctttgc cataaaattc
     2161 tgctgggatt tgatcactca gccagtattc acctgcgatg gacaccagtt ccgccatcag
     2221 gttcgtacaa aatatttgcg tttcccggtc accacttgcc cctgccagcg cacgaccgcg
     2281 catcatgcca tagacgtgaa tattaccatc ggcaatcaat tcggccccag cgctaacgtg
     2341 gcttgtaaca atcagatcac attgtggagc ataaatacgc tgaccggaac gcaccggggt
     2401 atctattaaa cgcgtttttg tgaccggcgt tgtattttgc gctggagcct gcggtgcggg
     2461 agctggacgc ggcgcttttt cctttccttc cgtcaggata ggcagcccca tcttttcaat
     2521 ttcggcttta agttgcgcat ctttgcagcc acttacgcca ataacccgca aaccggttgc
     2581 cgaaaccgcc ttatgcatcg ctgaccagtt taccgggtct tccagtgcac tgacgttgag
     2641 tacaacgggg gcatgtttta aaaatgcggg ggcctgagcg attttgtctt ccagcgcctg
     2701 atggataacc ttaggttctg cctcatgcag atgaaccaca gataaagtga agctactgcc
     2761 tttaagctcg attggcgtgt ttgacatcct ggccttactc aattagctat taatcatcgc
     2821 cagcgcgcga tgatgttccg aagactataa ggcatgttat agtctggatt atattgaggc
     2881 aagtcaccct cccatttatt cagagtaaaa gtctattctg tgataaatgg cgctgattca
     2941 tagcttaaaa aatacccttg tcaatcaacc cattgccgtc gtacttttga ttgttcttat
     3001 ttacgcttcc tttttccgca ccctaatcgt gacagcatct tcagacctgt ttaaatatcc
     3061 agtattgagg cgagtgtatt ttttcaccac gccaggcttt cttaactttc catttttgca
     3121 gcatcattaa agaacagata cccgttatct taatatccgc cacagaattg ctctatcata
     3181 gaaaccgcag aagacgtctg atacttctgt caggtcatcg taactcgtcc aggtcagcgg
     3241 ttttgattac ggattgatga tgaaatgagc ataagtgttg tacggttgga ttatcaggat
     3301 gtgctatgaa tatgatgcgt attttttata tcggattgtc aggtgtgggg atgatgttct
     3361 catcaatggc gtctggacat gacgctggcg gacttcaatc tccggcgtgc ggcgttgttt
     3421 gcgacccgta tatttgtgtt aactcagatg gcatttcccc agagttaaca aggaaatatc
     3481 tcggcgaaaa agccgctgaa aacttacaat cattacaagg ctacgatccc agcgaattta
     3541 cattcgctaa cggtgtattt tgcgatgtta aagaaaaatt atgtcgtgat gatcgttatt
     3601 ttggtgtgaa tggtaagcgg agtggaaaaa tcaatcaaac caccacaaag atgttattta
     3661 tgtgtcgtga gtgatgtctt gtttacatta tgttctattc gtcattaacc tcttgagcga
     3721 cagagaggag tggggcgtta acgtttatga cggggagagt ccccgtcggt tgatatttac
     3781 ttaatttgaa tatcgacgtt atatttttta gttttgtttt tacgggcgtc gtttcgggtt
     3841 tgcagcactc gcaactcata tttgccagaa gcagggagtg agtattgtcc gtgactatcg
     3901 agttctgggg aatatctgga taaatcaaca gaatcatcga ttcctggccc gaagaggtaa
     3961 gtatcagcgc cttcattaga gatactcaca tgcactttct gacctttttt ggcatagaag
     4021 gtatatgtat cgtaatcgta tccctttatt tcgccggagt actgagcgct gctatggcct
     4081 tttctgaact caacattgac attcttaccc gcagcaaagc tcgtggaggt tagcagtgca
     4141 agcaacaata cagccttact gatgccctta attttcattg gtagtctcct gttcagaatg
     4201 agtacgatga actgttataa tataacaatt catcacagga gaattccagt ggctttcctg
     4261 cgttcggtta aatcccggcg cggcattttt gccgggggga ttacttataa agggggcatg
     4321 gactgaacaa accgtaggaa tctacagctg gctggcttgt ggcgcgggtt tcattgatgt
     4381 tagcttatgc caaaacctgg catcttaaag agtaaaagta tgttttgtgt gatttatcga
     4441 agcagcaagc gtgatcagac ctatttatat gtcgaaaaaa aagacgattt ttcgcgtgtt
     4501 cctgaggaac tgatgaaagg ttttggtcag cctcagttag cgatgattct tccgctggat
     4561 gggcggaaga aactggtcaa tgccgatatt gaaaaggtta aacaggcatt aaccgagcaa
     4621 ggttactatt tgcagttacc gccaccaccc gaagatttgc tgaagcaaca tctttccgtc
     4681 atggggcaga aaacagacga cacgaacaaa taaccgatat ccggcggtgg cattatcttt
     4741 gtcggcgcgg gttttcatat ccacgataag gtgaggggaa cgttatgtat caacatcaca
     4801 actggcaagg tgcgctgctg gattatccgg tgagtaaagt agtctgtgtt ggtagtaact
     4861 atgccaaaca tattaaagag atgggcagcg cagtgcccga agagccagtg ctgtttatta
     4921 aaccagaaac ggcactgtgc gatctgcggc agccattggc gatcccatcc gatttcggtt
     4981 cagttcatca tgaagtcgaa ctggcggtat tgattggcgc gacgctgcgt caggctacgg
     5041 aagagcatgt ccgtaaagcc attgccggtt acggcgtggc gctcgatctg acattgcgtg
     5101 atgttcaggg aaaaatgaag aaagccgggc agccgtggga aaaggccaaa gcgtttgata
     5161 actcttgtcc gctttccggg tttattcccg cggcggaatt caccggcgat ccgcaaaata
     5221 caacgctggg cctgagcgta aacggcgaac aacgccagca aggtacgact gcggacatga
     5281 tccataaaat cgttccgctg atcgcttata tgagcaagtt ctttaccctc aaggccggtg
     5341 acgttgtgct gacaggcacg cctgatggcg tcggcccgtt gcaaagtggt gatgagctga
     5401 cagtcacttt cgatgggcat tctttgacaa ctcgcgtttt gtaatacttt ttgccgcctg
     5461 aaagcggcgg caaaacttgc atcgctgtgc cagactggtt ataaggtgcg ttttaacgta
     5521 atggcggaac acctgatgag cgatgtacct ttctggcaaa gtaaaaccct ggacgaaatg
     5581 agcgatgcgg aatgggagtc gttgtgtgat ggttgcggtc agtgttgcct gcataagctg
     5641 atggatgaag acaccgacga aatctacttc actaacgtcg cctgtcgcca gctcaatatt
     5701 aaaacctgtc aatgtcggaa ctacgaacgt cgtttcgagt ttgaacctga ctgcattaaa
     5761 ttaacccgtg aaaatctgcc aacattcgaa tggctgccaa tgacctgcgc ttatcgcttg
     5821 ctggcggaag gtaaagattt acctgcgtgg catccgctgc ttactggttc gaaagcggca
     5881 atgcatggtg aacgtatctc tgtgcgtcat atcgcagtga aagaatcaga agtgattgac
     5941 tggcaggatc atatcttaaa taaacctgac tgggcgcagt gataaattta atagatgttg
     6001 atgggggctt tcttaatata cgtctggata ctcgccattt cgtagccctg tttttgaaag
     6061 cgctctgtca taatgagagt tacccgatac cttattatca acaggtaact ctcaatcttt
     6121 aaaaaacggc tgtgttttta aatgtgataa accttatacc ttgagtacat ggagagaata
     6181 atcgcttatc agacttcagg tacctcaaag agtgtctttt taccgtgtct tttctgatac
     6241 tcattacagg tgttaatcat ttttttggcc gcttctttta gcaaagaaag cattaaatca
     6301 tcataatcaa catagaatct ggttgtttca gtttccgttt ttatctcccc gatggcggct
     6361 atttcggtgg ttaatttcaa tttggcggca tcgatatctt tattcgcttg tttaaccgtg
     6421 ttagacaggg tggtaaagaa actctgcaca gattttaact tgttcttcaa ttctggaatc
     6481 agtttccctt caactacgcc cgctgcaaca gaatacagtt tcttgatgcc gtcatccagt
     6541 accttaatga gaatgccttt ctgggcgaat gcttctattc tattttttag ccgggcgata
     6601 tttttcattt ctgctggatg agcgtcgtcg ccagaaggcc acgtgagcac aggataagag
     6661 aacgaaaaat cagcagtcta tgcagcgaca aatattgata gcctgaatca gtattgatct
     6721 gctggcaaga acagactact gtatataaaa acagtataac ttcaggcaga ttattatgtt
     6781 gtttatcaag cctgcggatc tccgcgaaat tgtgactttt ccgctattta gcgatcttgt
     6841 tcagtgtggc tttccttcac cggcagcaga ttacgttgaa cagcgcatcg atctgaatca
     6901 actgttgatc cagcatccca gcgcgactta cttcgtcaaa gcaagtggtg attctatgat
     6961 tgatggtgga attagtgacg gtgatttact gattgtcgat agcgctatta ccgccagcca
     7021 tggtgatatt gtcatcgctg ctgttgacgg cgagtttacg gtgaaaaaat tgcaactacg
     7081 cccgacagtg cagcttattc ccatgaacag tgcgtactcg cccattacca tcagtagcga
     7141 agatacgctg gatgtctttg gtgtggtgat ccacgtcgtt aaggcgatgc gctgatgttt
     7201 gccctctgtg atgtaaacgc gttttatgcc agctgtgaga cggtgtttcg ccctgattta
     7261 tggggtaaac cggtggttgt gctatcgaat aatgacggtt gcgttatcgc ccgaaacgct
     7321 gaggcaaagg cgctgggcgt taaaatgggc gatccctggt tcaaacaaaa agatctgttt
     7381 cgtcgctgtg gcgtggtttg ctttagcagc aattatgagc tttacgcaga catgagtaat
     7441 cgggtgatgt cgacgctgga agaactatcg ccccgcgtcg agatttacag tattgatgag
     7501 gcattctgcg atctgaccgg tgtgcataat tgtcgcgatc tgactgattt tggcagagaa
     7561 attcgcgcaa cggtgctaca acgtacccat cttactgttg gtgtggggat cgcccagacc
     7621 aaaacgctgg ctaagcttgc caatcatgcg gcaaaaaaat ggcagcggca gacgggcggg
     7681 gtggtggatt tatcaaatct ggaacgccag cgtaaattaa tgtctgctct tcccgtggat
     7741 gaagtctggg ggattggacg gcggatcagc aaaaaactgg acgcgatggg gatcaaaacc
     7801 gttctcgatt tggcggattc agatatccgg tttatccgta aacattttaa tgtcgtgctc
     7861 gaaagaacgg tgcgtgaact gcgcggtgaa ccctgtttgc aactggaaga gtttgcaccg
     7921 acgaagcagg aaattatctg ttcccgctcg tttggtgaac gcatcacgga ttatccgtcg
     7981 atgcggcagg ccatttgtag ttacgctgcc cgggcggcgg aaaaacttcg cagcgagcat
     8041 caatattgtc ggtttatctc cacgtttatt aagacgtcac catttgcgtt caatgaacct
     8101 tattacggca atagcgcatc ggtaaaactg ctgacgccca ctcaggacag cagggatatc
     8161 attaacgccg ctacgcgatc tctggatgcc atctggcaag cgggccatcg ctaccaaaaa
     8221 gcgggcgtga tgctggggga tttcttcagt cagggagtcg cgcagctcaa tttattcgat
     8281 gacaacgcac cgcgccccgg gagtgagcaa ttgatggcgg taatggatac gctgaatgct
     8341 aaagagggca gaggaacact ctattttgcc gggcagggga tccagcaaca atggcagatg
     8401 aagcgtgcca tgctttcacc atgttataca acgcgaagtt ctgatttact gcgggtcaaa
     8461 taaacatagc ggcaggaaaa agcgctcccg caggagcgcc gaatggatta gcgaccgaac
     8521 aggtcacgtt ttttcgcttt aaacggctgg gaaatcacca ccagcaccgc gacaatcagg
     8581 taagcgataa aaataccgag cagccactgc ggcatttcca gacctaaaaa ttcccactga
     8641 cgctcggcgc aatcgccaga ggcgacaaac acttgcggca cccatttatc cagcggcagc
     8701 cattccggga aacgagccat aaaatcacag gtcgcaaacg gcgaaggata gagctgaagc
     8761 atggtgtgct cgtaagttaa ctgcacaccg cggaacgcac tatacaacca gataaccatc
     8821 gctacataac gcagcggagt tttcggggcg atcgcgccaa tcagcgcagc acccagaacg
     8881 ccgaataatg cgcagcgttc ataaatacag agcacgcaag gtttcagtaa catcacatgc
     8941 tggaaccaca gtgccgtcag ttccagtgct agagcggtaa acgccatcaa cagccacgcg
     9001 ccccggcctt gtgaacattg gttcaaaaat cgcaacataa tcatttccct gcaatatgca
     9061 tagagtgcgc agtttaaacc aattcattcg ctgcgccacc aggaggcagc aaaaatagga
     9121 cgcaattgga caattatcgg gcgaaaaggc aaaccgggca tttatatgcc cggtaagttg
     9181 ttatcaaagc gttgctatcc agcccatttg cataaaccat tcggttacag gggcaagcgt
     9241 aaactcgacg cagagcaagc cgacgagtgt caggacgagg gtgtaaggca gggccatcca
     9301 caccatgcgg ccataagaga ggcgaatcaa tggcgcgagt gcagaggtca gcaggaacag
     9361 gaacgcagcc tgaccgttcg gcgtagcgac ggagggcaga ttggtaccgg tattaatggc
     9421 gaccgccagc agctcgtatt gcttcaacgt gatagcgcca ctttccattg ccgctttcgc
     9481 ttcgttgata taaatcgtcc ccacgaagac gttatccgaa atggatgaca gcagaccgtt
     9541 gaaaatatag aacagcgaca gctgagcatg ttccgatgcc tgcaacacaa actgaataat
     9601 cggcgaaaac agttgttggt cgataatcac cgcgactacc gagaaaaaca ccgtcaacag
     9661 tgcggtgaat ggcagagact cggtgaaggc tttaccgata gcatgttcat cggtgacacc
     9721 ggtcaatgat gtcgccagaa taatgaccga caaaccaatc aagccaactt ccgccagatg
     9781 caacgccagc gcagtcacca gccagacgcc aataatcgcc tggacaatca gacggatttt
     9841 atcctgacgg gtacgctggt ggcggctttg atcgtcaaac tgttgcagta cttcgcggac
     9901 tttctccggc agcgtttcac catagccaaa ccaacgcagc ttctctacca gcaggcaggt
     9961 taacaggcca caaatcagaa ccggaacggt cactggcgac atgcgcagga agaaatcgcc
    10021 aaaatgccag ccagccgctt tagcgatgat caggttctgt ggttcgccca ccatggtcat
    10081 cacgccgcct aatgcggtgc cgacaccggc atgcatcatc aggctacgca gaaagccacg
    10141 gaactgttcc agaaccactt tgtaatgctt gtcgatatga ctatcgtctt gcaggtcggt
    10201 atcttcggta cgggaagagg ctacgcggtg ataaatacca taaaaaccga ctgcaacgct
    10261 gatcaccacc gccacgacgg ttaaggcatc gaggaacgcg gagaggaacg ccgccgccac
    10321 gcaaaaagag agcgacagca gcattttgga gcgaatgctt aacagcaaac gggtaaatat
    10381 gaacagcaat agctgtttca taaaatagat acccgccacc ataaacatca gtaacagcaa
    10441 gacttcaaga tttgccgcca cctcttcacg gacgtgttcc gcgctggtca tgccgatgaa
    10501 taccgcttcg atagccaaca gaccaccggg gagcagcggg tagcatttca gggccatcgc
    10561 cagagtgaaa ataaattccg ctaccagcaa ccagccagcg acgaaagggc tgatgaggaa
    10621 aattaacgga tttacgatta agaaaatgat gagggcgagt ttgtaccagt cgggggactg
    10681 gcccaaaaaa ttgcgccata gtgcgcggcc ccaggagatc tccatgatgg tttcccttac
    10741 cttagaaata atcaatgatg tttttatgtt taaacgcaaa gcttaacggt cagacaggag
    10801 tgaggcaagt cttgatagtc aaggggaaag agatgcagaa aatgaagcct tgatcccttt
    10861 tttcttcttt ttgtcttcta tcagcatagt tagccctctg gtatgatgag tccaactttg
    10921 ttttgctgtg ttatggaaat ctcactatgg tcattaaggc gcaaagcccg gcgggtttcg
    10981 cggaagagta cattattgaa agtatctgga ataaccgctt ccctcccggg actattttgc
    11041 ccgcagaacg tgaactttca gaattaattg gcgtaacgcg tactacgtta cgtgaagtgt
    11101 tacaacgtct ggcacgagat ggctggttga ccattcaaca cggcaagccg acgaaggtga
    11161 ataatttctg ggaaacctct ggtttaaata tccttgaaac actggcgcga ctggaccacg
    11221 aaagtgtgcc gcagcttatt gataatttgc tgtcggtgcg taccaatatt tcaactattt
    11281 ttattcgcac cgcgtttcgt caacatcccg ataaagcgca ggaagtgctg gcaaccgcta
    11341 atgaagtggc cgatcacgcc gacgcctttg ccgagctgga ttacaacatt ttccgcggcc
    11401 tggcgtttgc ttccggcaac ccgatttacg gtctgatcct taacgggatg aaagggctgt
    11461 atacgcgcat tggtcgtcac tatttcgcca atccggaagc gcgcagtctg gcgctgggct
    11521 tctatcacaa actgtcggcg ttgtgcagtg aaggcgcaca cgatcaggta tacgaaacag
    11581 tgcgtcgcta tgggcatgag agtggcgaga tttggcaccg gatgcagaaa aatctgccgg
    11641 gtgatttagc cattcaggga cgataatccc ttccgtttaa aaagcaaacc cctcaaacga
    11701 ggggtttttc gttgttttta cagatttccc attctcggcg ggcaacgttc cagcaactcg
    11761 acgctgccgt cttcgttttg ctgttccagc atcacatcaa atccccacag gcgatgcaca
    11821 tgcttcagca cttctttgcg cccccgatcc agcggtgcgc gattatgtgg aatgtaacgc
    11881 agcgtcagcg aacggtcgcc gcgcaaatcc acgttccaga tctgaatatt cggctccaga
    11941 ttacttaaat tatattgaga cgataaccgg ttacggatct cccgataacc ttcttcatta
    12001 tgaatagcgg aaatctccag ataattatgc cgatcgtcat ccagcacggt gaagaagcgg
    12061 aaatcacgca tcactttcgg tgacaggaac tggctgataa aactctcatc tttgaaatca
    12121 cgcatcgcga aatgcagcgt ttccagccag tcggaaccgg cgatatccgg gaaccagtat
    12181 ttgtcttctt ccgttggcga ctgacaaatc cgtttaatat cctggaacat ggcgaacccg
    12241 agggcatatg ggttgatgcc gctgtaccac gggctgttat agggcggttg gaagaccaca
    12301 ttggtgtggc tgtgcaaaaa ctccagcata aaacgttccg ttactttccc ttcatcatac
    12361 agatggttaa ggatggtgta gtgccagaag gtcgcccagc cttcgttcat cacctgagtc
    12421 tgtttttgcg gataaaaata ctggctcacc ttacgcacaa tacgcaggat ttcacgctgc
    12481 catgattcca gcagtggcgc atttttctcc ataaaataga gcaggttttc ttgtggttcg
    12541 gacggatagc ggcgcgcttc agcaaccgtt ttctcttcct cgcgcttcgg cagggtacgc
    12601 cagagcatat tgacctgact ttgcagatac tcttcgcgac tcttctgccg ggctttctct
    12661 tcttgcagcg agattttttg cgggcgtttg taccggtcca cgccgtagtt catcagcgcg
    12721 tggcacgagt ccagaagccg ttctacttca tcaacgccat aacgttcttc gcactcggta
    12781 atatatttac gggcaaaaat cagataatcg acaatcgaac tggcgtcggt ccagctacgg
    12841 aataagtaat tgtttttgaa gaaagagtta tgcccatagc aggcatgagc catcaccagc
    12901 gcttgcatgg taatggtgtt ctcttccatc aggtaagcga tacacgggtt agagttaatg
    12961 acgatttcat aggccagtcc ttgctgaccg tgcttataca gccgttcagt ctcgataaac
    13021 tttttaccga atgaccagtg cggatagtta attggcatgc cgacgctgga gtaggcatcc
    13081 atcatctgtt ctgaggttat cacttcaatc tggtgcgggt aggtatccag ccggtagagt
    13141 ttcgccaccc ggtctatctc tgccagataa acatccagca ggtcgaacgt ccagtcgggt
    13201 ccatcgctca aacgtgtggt gtccttattc atagaatcga tcgtcgccat acgcgcacct
    13261 cattgttgtc ggcgctctct gtgtggagca cctcatttca agcatagaac acctgttaaa
    13321 aaccgcgtcg ccggagaatt tttttctttg cgatttctta ttatcagagt gccactaatc
    13381 cgcttctgaa cggaatttta tgctggataa aaagggcgtt cagcaggaga tactaaagac
    13441 gccatattgc cgcagagtca gggagatgtg agccagctca ccataaaaaa gccgtatgtt
    13501 gaataatatt ttcaactgag ttatcaagat gtaattagat tattattctt ttactgtatc
    13561 taccgttatc ggagtggcta tgcgagttgt catactggga agtggtgtgg taggcgttgc
    13621 cagcgcctgg tacttaaatc aggcaggaca tgaggtcacc gtcattgatc gggagccggg
    13681 ggccgccctg gaaaccagtg ccgcgaatgc cgggcaaatc tcccccggat atgctgcacc
    13741 gtgggcggca ccaggtgtgc ctttaaaagc gattaaatgg atgttccagc gccatgcgcc
    13801 gctggcggtt cgtctcgacg gtacgcagtt ccagttgaaa tggatgtggc aaatgttacg
    13861 taactgcgac accagccact acatggaaaa caaagggcgg atggtacgtc tggcggaata
    13921 cagccgtgat tgcctgaaag cattgcgcgc cgaaaccaat attcagtatg aagggcgtca
    13981 gggtgggacg ctgcaactgt tccgtaccga acaacagtat gaaaacgcga cccgcgatat
    14041 cgccgtgctg gaagatgccg gcgtaccgta tcagctgctg gaatccagcc gcctggcgga
    14101 agtggagccc gcgctggcag aagtggcgca caaactgacg ggcggcctgc aactgcccaa
    14161 tgatgaaacc ggagactgcc agctgtttac ccagaatctg gcgcggatgg cggagcaggc
    14221 gggggttaaa ttccgcttta atacgcccgt tgaccaactg ctttgcgacg gcgagcaaat
    14281 ctacggcgtg aagtgtggtg atgaagtgat taaggccgat gcgtatgtga tggcgtttgg
    14341 ttcttactcg acggcgatgc tcaaaggcat tgttgatatt ccggtgtatc cgctgaaagg
    14401 ctactcgctg accattccaa ttgcgcagga agatggtgcg ccggtatcca ctattcttga
    14461 tgaaacctac aaaatcgcca ttacccgttt cgataaccgc attcgtgtcg gcggaatggc
    14521 ggaaattgtt ggttttaaca ccgagctgtt gcaaccgcgt cgtgaaacgc tggagatggt
    14581 ggttcgcgat ctctatccac gcggcggtca tgttgagcag gcgactttct ggactggcct
    14641 gcgcccgatg acgccagacg gcacgccggt tgtcgggcgt acacgcttta aaaatctgtg
    14701 gctgaatacc ggtcacggca cgctcggctg gacgatggct tgcggttccg gtcagttgtt
    14761 aagcgatctg ctctcggggc gcacgccagc gatcccgtat gaagatctaa gcgtagcgcg
    14821 ctacagccgt ggatttacgc catcacgccc gggccattta catggcgcac acagctaagg
    14881 aaacgagatg acccgtccga tacaggccag cctcgatctg caggcattaa aacagaatct
    14941 gtccattgtc cgccaggctg cgccgtacgc gcgcgtctgg tcggtggtaa aagcaaacgc
    15001 ttacgggcac ggtattgagc gtatctggag cgcgctcggg gccaccgatg gctttgcatt
    15061 gcttaatctg gaagaggcaa taacgttacg tgagcgcggc tggaaaggac cgatcctgat
    15121 gctggaagga tttttccatg ctcaggatct ggagatttat gaccagcacc gcctgaccac
    15181 ctgcgtacac agcaactggc agctcaaagc actgcaaaat gcgcggctaa aagcaccgtt
    15241 ggatatttat cttaaagtga acagtgggat gaatcggttg ggcttccagc ccgatcgcgt
    15301 gcttaccgtc tggcagcagt tgcgggcgat ggcgaatgtt ggcgaaatga ccctgatgtc
    15361 gcattttgcc gaggcggaac atcctgatgg aatttccagc gcgatggcgc gtattgagca
    15421 ggcggcggaa gggctggagt gtcggcgttc gttgtccaat tcggcggcga ctctgtggca
    15481 cccggaagcg cattttgact gggttcggcc tggcattatt ttgtatggcg cttcgccgtc
    15541 cggtcagtgg cgtgatatcg ccaataccgg attacgtccg gtgatgacgc taagcagtga
    15601 gattattggt gtccagacgc taaaagcggg cgagcgtgtg ggctacggcg gtcgctatac
    15661 tgcgcgcgat gaacagcgaa tcggcattgt cgccgcagga tacgccgacg gttatccgcg
    15721 ccatgcgcct accggtactc ctgttttagt ggacggcgtg cgcaccatga cggtggggac
    15781 cgtctcgatg gatatgctgg cggttgattt aacgccttgc ccgcaggcgg ggattggtac
    15841 gccggttgag ctgtggggca aggagatcaa aattgatgat gtcgccgccg ctgccggaac
    15901 ggtgggctat gagttgatgt gcgcgctggc gttacgtgtc ccggttgtga cagtgtaact
    15961 tgttatcgct ggatgcgacc agcgtcgtat ccagcgttac aaaacacatt aaacttaaga
    16021 ttcggcttct tcctccgcta cccgaacacc aatcttcaac acttcattat cctctttctc
    16081 ggccaccgtc cagatcattc cggcaaattc tacctgatca ccgacaaccg gtgccgcgcc
    16141 taacaactgc tggacaattt cacccagcgt ttgctgctta tcacgatatt ctcgcccgtc
    16201 ttccaggcca tatatcagcg ccacatcagc atatttggca ctggcttcga gaatgaagtc
    16261 accaaagaag cgttgatcta gcgcgaccgg tggcgactgg ctgaacagtt taccgagcgc
    16321 cgggagatcg cgttcccgac caattacaca caacacatcg ccttcgcgca gtcgggtgct
    16381 gccggtagga tgaagcaact ggttatcacg aaacagtgcc gcaatacgcg tctcttttgg
    16441 catatgcaga tcgcgcagtg ccgcgcccac gcaccattta tcggcactca actgataaac
    16501 aaactgctcc cacggatttt ccggatgaat atccagacca acgcgtgaca ccggacgtcc
    16561 cactggcgga acgaccactt tggctttttt cgccgcccac gagagtgatg tcccctgcaa
    16621 gagtagtgaa accagaacca caaagaaggc gacattaaag aacagtcgcg cattctccag
    16681 ccccgccatc atcgggaaca cggccaggat gattggcacc gcgccgcgta atcccaccca
    16741 gctgataaac acgcgctcac gcagattgaa gccacggaac ggtagcaatc cggcaaatac
    16801 cgaaagagga cgggcgaaga aaatcatcca tgcggacaaa atgagcgccg gaatggcaat
    16861 aggcagcaga tcgcttgggt taaccaacag ccccagcacc aggaacatgg cgatttgcgc
    16921 cagccaggcg aggccgtcga aattttgcag aatgccgtag cggttgcgaa tcggccgatt
    16981 acccagcaga aaaccgcaca gataaacagc cagaataccg ctgccttcca gcgccgtggt
    17041 taaagcaaaa attaaaatac cgccgcttaa tgccagcaat ggatataaac cggcgggcag
    17101 ggcgatgcga ttaatcattt gcagcagtaa ataaccaccg ccaagaccga tgacaatccc
    17161 gaggccaaat tgctgcaaaa tatcgaccac gaacatccag ctgacactgc tttcatgctg
    17221 ctggatcatc gcaatcaggg taatcgtcag aaagaccgcc attggatcat tactgccgga
    17281 ttcaatttcc agcgtcgagc caacacgttc gttaagcccc ttaccaccca gcagagaaaa
    17341 gaccgctgca gcatcggtgg agccgacgat agcgccgatt aataatcctt caatcaaatc
    17401 aagattaaac agccacgccg ccatcatgcc ggttaaacca gaggtgataa gcacgcccag
    17461 cgtcgccagc gacagtgccg gtcctaacgc cacacgaaag gaactggcct gagtgcgcat
    17521 cccgccgtcg agcaaaataa tcgccagcgc cagattactt accatgtagg cgaagggata
    17581 attatcaaac gggataccgc cgacgccatc aacccccgcc agcatgccga tcgccagaaa
    17641 aataaccaga atgggaatgc caagacggga agaaaatgaa ctaagtaaaa tactgctggt
    17701 gacaaggatg gatcctaaga tgaaaaggct aattattgtt gtggcatcca acggtcggtt
    17761 actcctgatt acgctgtctc ttatataaac cctaccatat tagcggcaga gacagcgttt
    17821 tacttagccc tgaagcgtga tttttttaca ttttaagaac aggatgaccg ctaatcgtca
    17881 actgggtgcc ttcctgggtg ttattcagaa tggcatgtgc gcccaacggc agtgtaaccg
    17941 tgcgttgttc atgaccaaaa tcgaggccgg taatgagtgg aatcgacagg cgggaacgca
    18001 aaaacgcgta cactgactcc aggttgtaac ctgcgtcata atcattaggc gtgctgccgc
    18061 taaagctacc gagaataatc gccttctgac gtggcaaaat tccagcatga tagagctgca
    18121 acagcatacg ttcgacgcgg aaagggtgct cgttaatatc ttccagcacc agaataccgt
    18181 tctcaatttt tggcatccac ggtgtaccaa tcagtgaaat cagcatcgcg agattgcctc
    18241 cccacagcgt gccttcggtc tgacatgtcg ggccttcccc ttgccattca atggtgaagg
    18301 tttcattgcg taacgccagc cagaaatggt gctcggtaaa ggcgttcagt tcatccgcgc
    18361 caaagtttgc taccagcatg gggccgctaa aggtgatgac attgccatgc gccagaagac
    18421 cgcactgaat ggcggtaaaa tcgctatgtc cgcaaatgag caacgggtca tgttgttggc
    18481 gagccaccag tgcctgccag tcaatatctg ccagtaaacg actggcaccg taaccgccgc
    18541 gtacagccag cacgatggtg ttgggggtag tcagtctggc aagagaattg agatcttcca
    18601 gacgctccgt ttccgtaccc gcaaaacgct cacaacgacg ggcaataacc tcgacgttat
    18661 tgacctgatg ccccgcgtcg gtaaggcgct ggataccacg cagcgcggcg tgctgtttaa
    18721 tgcagtaacc cgatggggca attaagtgaa acagagacat ggcaattcct tgctgacaac
    18781 agaaacgaaa tgtatatcat gccgcttagg tgtgccgttg tcacctcaac ggcgattcca
    18841 ggctataagg atagaagaag tgaaattgag atggtttgcc tttttgattg tgttattagc
    18901 gggttgttca tcgaagcatg actatacgaa cccgccgtgg aacgcgaaag ttccggtgca
    18961 acgtgcgatg cagtggatgc caataagcca gaaagccggt gcagcctggg gcgtcgatcc
    19021 acaattgatc acggcgatta tcgctatcga atcgggtggt aatccgaacg cggtgagtaa
    19081 atcgaatgcc attggtttga tgcagttaaa agcttcaacc tccggacgtg atgtttatcg
    19141 ccgtatgggc tggagtggtg agccgacgac cagcgagctg aaaaatccgg agcgtaatat
    19201 ttcaatgggg gcggcttacc tgaatattct ggaaaccggc ccgctggcag gcattgaaga
    19261 tccgaaggta ctgcaatatg cgctggtggt gtcatatgct aacggggcag gtgcgctgct
    19321 acggactttc tcgtcagatc ggaaaaaggc gatcagcaaa atcaacgatc tggatgctga
    19381 cgagttcctc gatcacgtag cgcgaaatca tcctgcgccg caggctccgc gctatatcta
    19441 caaacttgag caggcactgg acgcgatgta aatcagtcgc gcactttgtc cgctttctcc
    19501 cgggcttctc gctcgagaga gaaaataatc cgctgtaatt gccgctccac cgtcgggctg
    19561 acgttaagaa aacggaagct cagacgggga gtggtgatgg tttcattctt gccatcaatc
    19621 actttgcgct cgctgatgga gattaactgg gcgtcaaagt gaaaaacacc ccattgcccc
    19681 atgttgactt caatctgagc gaagcgcatg ccttcgtgca atccggcagg ctttgctgtt
    19741 tccagtaatg cgcccatgcc gcctaacgac aaatcataca ggcggaaacg taacgtactg
    19801 ttatccgcca gtttggtctg gcaaaaatat ggcggatgga gtggggcgga gatgcggaaa
    19861 tatcggcgtc gttgtacaaa ccacaaggtg ggaggcggta cggtaataaa tgccggaagc
    19921 tgcaagtatt cactctgctg tagttgttca acagtaaact cgactttcgc accctgggtt
    19981 tcggcggtaa tggtaatgtg ctgcgccttt aacacagcgt tgttatcttc ggcttgactg
    20041 ccgaaatcca gcaccagttt atccggggtt attgccagta ttttgctgat cagctgcccg
    20101 ccattccaac tgatacgcaa aggaattgcg gctttgtgca aatcgcgtaa cacgcccagg
    20161 acggctaacg gattttgttt caggaactgc tcatggtaat gactcacgcg gaaaaactcc
    20221 tgactgacaa actgtcttag gtttatcggt cacagttaac aaaacttaat acaaatgcgt
    20281 gaatattttt tacatgtcgt tcttaaatca gccgacatac gcccagcatc gatcccgccc
    20341 ttgcctatac ttagagcgtt gacgtaagca tttcttgcgt ccgattcatc gaaacgaggg
    20401 cctgaacatg ggaattattg cctggattat ttttggcctg atagccggca ttatcgccaa
    20461 gctaatcatg ccggggcgtg atggtggtgg atttttcctg acctgtattc ttgggatagt
    20521 cggtgcggtg gtcggcggct ggctggcgac catgtttggc attggcggct ccatcagcgg
    20581 ttttaatttg cacagcttcc tggtggcggt agtgggcgct atcctggtgc tgggcgtatt
    20641 ccgcctcctg cgaagagaat aagattttca taaggcggat agcgatacag atgccgctat
    20701 ccgctttcac atcagaacgt atactcgata cctgcccaga aattgcgacc ttcttcaata
    20761 aacccttcac tgtaggcata tttggtatca aacaggttat tgaccgacgc attgacgctg
    20821 aagccatgac ctaaggtgta atcggctcga atgtgggtca ccgcaaaacc ggcggctttt
    20881 tgtgaaccgt cactgttgct gtagctggag gaacgcgcct cttccgacag cgttacgctt
    20941 aacggctccc acggtttgag agtcatccat gcggtcattg tctgcgttgg cagatcggtt
    21001 atcttgccga tgtctttgcg tttggcatca gcgtggatca gggcgtagct caatcctaca
    21061 tccagtatat tgctgatttt ccccttaata ccggcatcca gaccgctgta atccaccgtg
    21121 ccgctgttct gattttgaat ggtatcggca tcgatattgt gcgagaggat ggcatcactc
    21181 acccggttat agtaaacgct gacctcaaac ccccagtcgt gcgtgaaggc accattccag
    21241 gttaaatcca ccccgcgcgc gcgttccggt ttgagctgcg ggttaacaat cgctatctgg
    21301 ttatacgcag gtttggacgt ggtatagcgt tctttcagcg tcggaaagcg tgtgcggtca
    21361 tagtacgaaa gcgccagcgt gtcttcattg gcaaagtggt atttccccat cacctgccag
    21421 ttaaaagctg actgattgtt gtcgtcataa tgggtgatgc ttccatcttt ctcatgtttt
    21481 ttcgcttcta cgctatcgcg ccagtcatag ctgattccag ccacgacatc gacattatcg
    21541 gcagcagccc attgatattc actggcgagc gaccaggtac gatcttcata gcgatcgtaa
    21601 gcggcgtgcg gcgcaccttt ttcccggtgt acgtcatctt tccagttaac ggcaaacgac
    21661 agcagatcgt tttcacgcac atcggctgcc agttgtaacc cggcaccgtc gctgtaatcg
    21721 gaataatggc tgtagctgcc ttttttattt ttcaaatcag ccagcgagtt gtacatcatc
    21781 agcgtatttt caaaggtatc gcgatacagc cgacttttca gggtaaaacg atcgtttagt
    21841 tgggtcgttc cctgataata aaaactttct ttgtcatact ctggccactg ccagtagcgt
    21901 gatttttgac cactatttcc actgtatggc ggattatctt tttcaccatc ctgcttaatg
    21961 taagtcaatg tgtattcatc gttttcgcgt ggtgtaaaac cgagcttcac aatgccgcgt
    22021 ttatcatcag ccgatgaatt aatcatcttg ccgtgtttgc ctgcaatatc attatttaca
    22081 ccatgcggca ggccgagaaa atcctgcttt agctggctac cgctgacttg caaatacccc
    22141 agatcgctgc tggcggcaaa tgaagcatgc atatcgtagg cattgtcctg gctacggctc
    22201 catccctggc gatatcccag atttgcttcc agaggttttg ttggcttctg ggtggtgata
    22261 ttaatggctc cgcccatctg attaggcccc tgaagcagcg acgaataccc tttggaaact
    22321 tcaactgccc ccaaattgtt ggtcagaatc cgcgccagat cgaggttgcc gtcataggga
    22381 acataaatgg gcacaccgtc gaaatagact ggcacctgac gactatcaaa gccacgaact
    22441 ttgacctgtt cttcgttgcg gctacctgac ttttgcagca ccacgccggg gacgacactt
    22501 aatgcctgag cgacattctg tttatccagg gccttcatgg tggggtgatc gagaacggtg
    22561 gtcgtcgtcg atgataccgg actggaccat acggtcagcg tgtcactctc ctgagccgct
    22621 aaggcctgct gggtaaaggc aagtgataat gctgtgcaga gataacgttt tttgagtctc
    22681 atagaataaa ttccgtttaa caaagaatta aatagaggct gcgggttgca gactgacttt
    22741 aatgtcagct ggtgtcgcgt aataaggcgc ggaggtaata aaaaggcgaa tgccgcagtc
    22801 gaggtaattt ttgagtgttg tcagattaat tccgccggtc agagccagcg tgcagtgagg
    22861 agccagcgac ggtgctattt gcgcaatttc agttgcctgt tgcggattaa atttgtcgag
    22921 ctgaagtacg tctggttgcg cgcgtaacgc ggcgattgcc tctttcggcg tgtcggcttc
    22981 aacaactatt ttcttctcgg gtgcgtggcg gcgtaactga ttgattgcgc ctgaccagtc
    23041 ctgattgtca tgaagaaaat gacggtggtt ggcaaacaat aatatggttt ccgcacatcc
    23101 ggcacgatga atcagtcctc cggcagccag aattgcctgc gaggccagta agcgggtgcc
    23161 cggaattgct tttcgggtgc aggcgatatt gccatcaggg taacgttcac gaagtaacgc
    23221 cagcatttga gcgagataat cagaaacacc gcaactccac tccagcacat tctggactgc
    23281 cttccatccc tgatgaagtg ctgcggcatt accctgcgca cggattagcc gctgacccgc
    23341 gttcgcttgt gaaccgtcgc tgactgcgtc atcaatagtt aatcccagcg tagttaacat
    23401 cttacacgcg acagaaattc cgctgacgca gccgccctga cggtgaaaaa actctatata
    23461 gccatgctgg tgtccaatat ttaatgctcg cgtggtcagg tcaccgccct ggatatcttc
    23521 cagaagtaac gcatcgattt gtgcctgaga aaggaaaatc atcgcagtgc ctcttgcggt
    23581 acgctatccc accagatgag cgcccagtcg cggctggcag gggcgagggc acgagcatct
    23641 tgttgttgat accagcgata taaccgctca cgctcatcgt cgttaagcgc gcccagactc
    23701 cagctgacgt tctgctcaaa acgttcccag gtgctgttat cctgttggca attttgtccg
    23761 cggataaaat caacgtgagc ataaattccc atctgataca ggacgttgag cgcaaagata
    23821 tagttgggta attcgaccac ttcccgacca gccgcccgct gaatggctgg agagacgaac
    23881 gaggtgctga ccagatgagt cgtatagacg cgaagccgtg cctgattatt cagtttgctc
    23941 attgcctggc gcatatcagc caccagagtg gagcgggaag ccacggcaat atcgcagcgt
    24001 ggcaggtcac tccagtcctc ttcccaggcg cgctggatcc agtggacatt gtctgccttt
    24061 agcgcggcgg cgcgacgggc ggcaacgttc agcatcccct gactgtaatc aacgccataa
    24121 atcgtcgtga gtttgtccgc cagcgccagc gatacggtac ccggtccgca gcccatatcg
    24181 aataaggttt gcgcgccttg caggtcaatt tttgaggtca gttgttgcag gtagctgtcc
    24241 gtcggcgagg cgcaattttc cgccattttt tccgcccgtt gatcccagtg atccggcgtt
    24301 ttttctgtgc gatgggccag cctgagttgc tgaaggtaaa ggtctgcaaa atcaatatcg
    24361 tcaatgagca tgatttttcc ttagtgctta attgcagaga ggtgatggtg aatctggtcg
    24421 gcagaaaccc ggtacagggc agccagtttt ttcgttgtta actgttcggt cggtaagcct
    24481 tgtgttactc gtccatctgg ctctacctgg ataatgctgt cggcaatggc gtttgcatgg
    24541 agcggatgat gagtcgacat taacattgtc atgccgttat ttttgagctg cgccagcgtg
    24601 tccagaagtt ggatctgatg accgaaatcg aggctagagg caggctcatc cagtaataac
    24661 aagcgcggtt gctggacgag tgcacgggcg attaaagcca gctgacgttc accgccgcta
    24721 agcgtattcc agcgtcgtgt cgccagatgc aaaatgccca gcttttccag ttgctccgtt
    24781 gctttcaggc gttcttgctt accgggaacg gaaaaagcgc ctatggtggg ggcctggccc
    24841 atcaacacca tatccagcac actaaaagca aaaatgccgt cgtgcgcctg cggcacccag
    24901 gcaatagcct gcgcgcgctg gcgatggctg agtgtctgca cagggaggct atccagtaag
    24961 atctcgcctt ttaataacgg tataacgcca agaatggagc gcatgagtgt ggttttgccg
    25021 caaccattgg ctcctaacag acaacaaatt gttccttgcg acagggtgaa ggacacgtcg
    25081 cgtagcaccg gatggcggga gtaacccaga gacgcatgac ggacggcaag caaagtcact
    25141 ggcgacctcc gcgtaacaac aaaaacagga aaaacggtgc gccaacaaaa gcggtcagaa
    25201 tgccaagtgg gatttctgtt gttccaatgc tgcgtgccag cgtatcggta agcaggagca
    25261 gaatcgcgcc agtacacatt gccatgggca acagctgctg atggttatga ccggtcaaca
    25321 agcgggcaat gtgcggaacg accaggccga cccaaccaat tatgcccgct attgctaccg
    25381 tgctggcggt gatgagtgtg gcacagacaa tcaatccaaa gcgcagacgt gtcacgttca
    25441 gtcccagcga acgggcttcg tcgtcggaaa gcgtgagtaa attcatgcgc cagcggagaa
    25501 aaaacagggg caaggagcct atgagaatga tcggtgcagc atagcagaga tcgcgtaagg
    25561 tcacggtgga cagccccccc agcaaccaga aggtaattga cggtaactgg gtataaggat
    25621 cggccagcgt ttttattagc gatatccccg ctccgcacaa cgttcctaac gcaatgccta
    25681 ccagcaccag ggttaagatc ggatcgtggc gggtcacccg acgcgtaatc aaccagacgc
    25741 ccgcgaccac catcagcccg ccgcaaaatg cgtacagctg gatgtaaaca atggataatc
    25801 cccacaggat tgccgcgcag gcaccaagtc cggcaccggc ggagacgcct aaaatatccg
    25861 gtgacactaa gggattacgg aacattccct gataggtggt tccggcccct gccagcgccg
    25921 cgcccagaaa cagcgcagca agaatacgcg gcacgcggat ttgccagaag acaatttgtt
    25981 cctgcacagt aacattttct tgacgaagaa tgagcgccag cagttgctgc atatcgagat
    26041 gataagcccc acttacggcg gcaaaagtga tactgatagc tgccagcagc aaaatacatc
    26101 cgtttacggt tctcattagc tcgccaccaa tttttgatag tcggcgtcac ttaatgggca
    26161 atgccagaac agttgggcgt aatgctgcat gtcactttta aactggcgat tgatggcggg
    26221 atccagccag gcgtgaagtc tgcgtaatcc cagaagacgg ttgattcccg gcggggcatc
    26281 cagccagcca aagggcaggc cacttaaaaa taagatgcgt tgttccgcaa ccgctttcac
    26341 gccctgccag agaggatcac gacgaataaa atctgcagta acggcctcct gaaccagaat
    26401 aatatccggc tgccagcgca ggagattttc catggaaacc tgtgtcagac cgtggcgatc
    26461 ggctatttgc gcgacgttgt gcaaacctaa taattccgcc gcctcggtat gcaacgaacc
    26521 ctgtaagccc gtttccagcc ctcgaggacc gcgcgcagca taaaagctga gattagcggc
    26581 gggtgaggtg gcgaatgcct gagcttcacc aacgaagcgg ctggcgagat tggcttgttc
    26641 tgcggcgcgg tgctcttcgc ctaacgtttt gccaagcgtt gttaactgtt ctgctgattg
    26701 ttccagtttc ccgttaagca ataaccaggg tatttgtgtc tgttcgctaa cctgccgtgc
    26761 ctgggagatc caggtttcat ccgtattgcc gcaatcaacg accaaatcgg gatgtaacgc
    26821 cataagccct tccagcgaga gtgtactggc gcgcccggcg agtcgtccca gcctggggaa
    26881 ctggcgaatg tgctctggca atggaattaa tgcctgacgg gcaaaatcaa aagaggaaaa
    26941 accgaccatt ttttctggcg cgacagccag caatagtaaa tcggcgggcg cgcctgcgct
    27001 gacgatgcgt gtaatgtttt gggcttcggg aagcgtcagt gaggcccaga atttgactga
    27061 ttgtgcaaag gacggcagat gatgcaggaa caatgtcgac gcaagaatct gtgtaaacct
    27121 ccgtctggtt agtgacataa gtaatatcct gataattaaa accgctatat acttacacat
    27181 atagcgaaaa agatcatgtt tgatgtcaaa atttgtctaa agagacggaa tgatttgctt
    27241 gttgcttaaa taacactttg ttccacaaga aaattgtcag ggagaatggg gagtggggga
    27301 aaatataaat agaggtgtaa tgattaacaa gttgataaaa taaaattaaa atcttctcta
    27361 ataaaagaaa ataaaggcat agaccgtaga aagagtggta tttggtattt cgtggtgaag
    27421 aaagagcaat gccaatttta tgcacgtaat ggcattgctc gatatacagt aaagttaagg
    27481 cgtaggttcc gcctcatttt gttttaccgg ctgtgttgtt gattcactga gtggacgcgt
    27541 cgccggaaca ttgtcgcacg gttgctcttt cgggcagatc aaatccagca ttttcagcgt
    27601 cacgccattg gtccagccaa agccatcctg taatggatat tcgccaccgc cgccccctgt
    27661 tccggtggtg ctgacatcat atttttccac cagctttttc tcccggtcat aggtgtgctg
    27721 aacattggtc aggaagtgcc agctaatgtc catcgccacc tctttttgcc cgtagttttg
    27781 taatccttct gtcgcgaccc actgcaacgg tgcccagccg tttggcgcat cccattgttg
    27841 cccacttttc accgacgtgg tgttcaggcc gcctggttgc agcagatgtg ttttcgtcgc
    27901 cgtcgccatt ttgctggcgc gatctttcgc tgccgcattg acgtacagcg ggaacagggc
    27961 ggccgcggtt aactgattgc gcactttatg acttttcagg tcgtaatcgg cataccagcc
    28021 ttgttgatcg ttccacaggt atttttcgat ccctttttga cgggcattcg ccagcgtttc
    28081 gtactggttt gccatcgcgt tatctccggc agctttgctg gcgcgggcga ggattttttc
    28141 cattttaaac atcaggctgt tcagatcgac cggtacgatg ctggtggtgc gtaaggtatt
    28201 taactgctgc ggattgtcca tccagcgcga gctgaaatcc cagccagacg cagcggcaga
    28261 gcgcaggtcg cggtaaattt cagtggcagg tcgattcgga ttgcttttgg cggtggcaat
    28321 atcttccacc catgactctg gtcgtggcgt atcgcgatcg tcccagtagc ggttgagaag
    28381 ggtaccatcc tgaagtttga caacgcgttt ttcctgttgt ccggcttgca ggttttcaac
    28441 accgtccatc cagtaagcat attctttttg catttgcggc aggtattgct tcaacgcggc
    28501 atcgccttca tgctgcgcca gtaactctac catcagggca aagaatggcg gttgtgagcg
    28561 gcttaaatag taactgcggt tgccgttggg aatatgcccg taagtgtcta tttcatgagc
    28621 aaaattggcc accatatccg cgactttatc ccagtgacca ctttcggcaa gccctaacat
    28681 ggtgaagtaa ctgtcccagt aatatacctc gcgaaaacgt ccgcccggca caacataagg
    28741 ttccggcagc ggtaacagag aatcccattt ttcggtattt tcggtagaac gcgttaatac
    28801 cggccaaagt ccgtcaatat gttcgcgtag tgattgcccc tctggcggaa catatttctc
    28861 gccctctttc ggcagggtga aattgacgtt aacgaaatgg cgtagatcaa atccgctctg
    28921 gttttgctgc atccgataat cagcaaggat catcagcgga tcgctgttcg gcacggcatc
    28981 ggcaaaggtt ttttggtccg gaaaaagttt ggcgttttgc acatcattaa acagcggccc
    29041 taataaaata tcaggcggct gtggcgttac cggtgtttct tctgcctgca ccgatagcgc
    29101 agcgaaacac aaaaagatgc aggctggaat taacgccatt tttagcgggc gagaagctgc
    29161 gggggatttc atcaatcatt ctcctttggc gaaaccgaat aaaacggtat tcaagtatca
    29221 gaaaacctta gttcaggatc gcactagctg catgatcgaa atctcattct gcgaacggtc
    29281 aggcatttta gaaaagggaa ttaaagggat aaataacgtc gaaatagagt gatcaaataa
    29341 cgtctgtttg ttagccagcc agcggcgttg ggttcgcgat tagcgcagaa aaaatcatca
    29401 tgatttggta aaaaatataa caggtagtta aaaatcatcc gtgcagagta aatagccgga
    29461 tggcatcaga acgatgccat ccgaacggtg gtttaaccct gacggctgaa acgttgcgtt
    29521 ttaacgtcca gcgttagcgt ttcttctggt tgtatcgcat acagtttctc accctgctgg
    29581 caaatccagc caatccccag ttcacgggcg atcagggcgc tgtgggatag cggacttccg
    29641 gcgctaaggc agataccttt tacaaccgcc ggatccagtt gcagtattgt ggagggatag
    29701 atgttctccg ccagtagaat agttggcgag ttaaactgcg ggagttcttc tttcgtttgg
    29761 gtcaggtgga ccagggtgcg atgcagaaga tcgtccacat caatatagcg agcctgtaga
    29821 tattcatcat ccagttgctg gtattgctgg ctaagttctt taagaacttg ctgccaggca
    29881 tattctgccg tgcaatgttc atgctgaagg agttcgcttg ccgccgccag cagttccgga
    29941 tcatctaaca gtgtatggtg accagaaaag attgcggcaa tatcgtcaag cccgctggct
    30001 tctgctttcg ctgttaacgt catcagatct aataacgtga agtcaatggc ctggcgtaat
    30061 cgttcttgtt cttcatccac ggtcagggtt gattttgcct gtaccgtaca taaaaccggt
    30121 tgataataaa aggctttacc cgaaacaggc ggaacgggac gcagaatagg tggagcaact
    30181 tcctccgttt caccaaagtt atcttcagcc agctgacgga aagcgatcag tgcctcttca
    30241 gcttctggcc ctttcgcaat caggcgcagc gtatcgttat agcgaacttg tagtaacgca
    30301 atctggttaa tactctccgg tgtgacgcat ttgccgtttt tctccagcaa catatcggca
    30361 ttaaatgtcg ataaggtata aaccagccgg gaggccggac gtacatgcag gccgttacgg
    30421 ttttttatga ccaccgccag agaacgggct tcttcatcgt acgaaggaca tgtgtcagag
    30481 atttcagtgt tggaggaggg taaacccagt tgttcacgtt tggcttccag cgcatgcatg
    30541 gcgtcaaaga tcactttgtc gatctccgcc cccgaggccg cgctaaccgt tgctgccagt
    30601 gtaccttcga ccaacggcgc agcacacaaa cgtacttttg cggcgatctc gggagccagc
    30661 aattccagcg cagtttcagc actcaataat gcgcttccta tatccatcat gaccagcaca
    30721 tggtcggtat cggcaacaga ttcgatggcc tccatcactt tgacggcatc ggtaccaatg
    30781 ggattttgtg gatcgtcaat tcccgcggca atggcgattt tacaattatc actcattaac
    30841 atctgacggg ctaattcacc gacgccttct cccagtcggc tgctatgtga aactatgacc
    30901 aggtttacca tcaccaattc cttactcttt tgcggctaac gccaacattt gcatcataaa
    30961 catcaccgag gtcgcaccgg ggtcctggtg accaatactg cgttcaccga gataactggc
    31021 acggcctttg cgagcttgca tcgtaatcgt actttgtgca gcggattcgg cgacgctact
    31081 ggcagcttcg agcgccgccg gaatagagag attttgctcg ctggactgac gtaacgattc
    31141 caccaccggc acccacacat cacacatggt tttatcgcca ggttcggctt tcccgcgact
    31201 gattacgccg tccgcgccat cgcggaacat ctgataaagc tcttccagcg tcaggctttg
    31261 ccgtgcctgg gtcgcctgtg cggcgcggat aaagaaggta ccgaaaagcg gaccactggc
    31321 accaccgacg ctggaaagta gcgtcatacc ggtattcttg agaatgaaac cgatatcttt
    31381 atctgcgata gcagggagtt tttccaccac tttgctaaag cctcggttca tatttagccc
    31441 gtggtcagcg tcgccaattt cgcgatccag tccggtaaga tactcgctct cggtgctgaa
    31501 aatatcgcca caacgagtga gccagttaac aatttgagtt ctgctcagtg acattgctat
    31561 ctccttattt accccagtta agggccgggg tgtggaccgg ggcgtcccag agtgccagcg
    31621 tttcgtcatc aactttcagt aaggtgattg agaaaccggt catatccagt gaggtgcagt
    31681 acgcgccaat taaattacgc tcgatagtca atcccgcttg ctggcaacgt gtggtcaggc
    31741 ggttatagac gccgtacagc tcagaaagcg gagttgcgcc aagattgtta accagcgcaa
    31801 tcacccgatc gccagactgg agcggttgtt tagtttgtga ttcttcctgc caactgcctt
    31861 gttgataatc ccagaaacgc aaagtgcgat ggtatgagcc attttccagc agggtgtcga
    31921 acatttcatc gacggtttga tcaagggaag agaagggacg gcggtcaata cctggctcac
    31981 catgaatgcc gacgccaaac tccatctcat tgtccgccag ggtaaaagaa ggtttgcccg
    32041 cggcaggaac ggtacaggca ccgagagcga tacctattga gtggccttga ttattcaact
    32101 tacgccccag ttccgcacag gcgtccagtg agtcgccacg ctcggccgct gcgcctacga
    32161 gtttttcaat taatacggtg ttggcaacgc cgcgtcgccc ggcggtataa agactgtctt
    32221 ttaccgcaac gtcgtcatca atgaccacag tggtcacttt tacgccgcta tcgtgcagta
    32281 actcggtcgc tgtttcaaag ttaagaatat cgccggtgta atttttgata atcaacagta
    32341 caccttcgcc gccatcaatt tgcatggcgc attcaaagat tttatcgggc gtcggtgagg
    32401 tgaaaatttc gcccggacag gcccccgaaa gcatcccctg accgatataa ccacaatgca
    32461 tcggctcgtg tccgctgccg ccacccgaca gcagggcgac tttacctgca acgggggcat
    32521 cagatcgggt gacatacacc ggatcctggt gcagtgtcag agatggatgc gctttcgcta
    32581 gtcctgccag ttgttcgtcc agtacgtctt gcacatcatt gatcaatttt ttcattattt
    32641 tgctccagca attacggtag ggcatggatg atgttcaacg acacggcgac cactgactgc
    32701 cgatgaatcc attgtgcatc aggcaaggga aaagaaaatt agcccaaatt atgtttcata
    32761 gtgaaacata ttctttaatg aatgttccat attgaaactt ttacgtgtat taatacttaa
    32821 aattgcgagc cggaacacct tttgtcataa gggatgcggg atatgagtag cgcttttaac
    32881 aacgatggtc gggacatatc tcccttaatt gcaacctcct gggagcgatg cattaagctg
    32941 atgaaacggg atacatggaa cgtaccacac caggcccagg gcgtgacatt tgcgtctatt
    33001 tatcggcgta agaaagcgat gctgacgctc gggcaggctg cgctggaaga tgcctgggaa
    33061 tatatggcac cgcgagcgtg tgcgctgctt atcctcgatg aaaccgcctg cattctcagc
    33121 cgtaatggcg atccgcaaac cttgcagcaa ctaagtgcac tgggattcaa tgacggcacg
    33181 tattgcgccg agggaattat tggtacttgt gcgctatcgt tagcggctat ctctggtcag
    33241 gccgtgaaaa cgatggccga tcaacatttc aaacaggcac tctggaactg ggccttttgt
    33301 gcaacgccgt tgtttgacag caagggccga ttgacgggaa caatagcgct ggcgtgtccg
    33361 gttgagcaaa ctaccgcagc tgatttgccg ttgacgttgg caatcgcacg cgaggtcgga
    33421 aatttactgc tgacggacag tttgctcgct gaaactaacc gtcatttaaa tcaacttaat
    33481 gccctgttag aaagtatgga tgatggcgtg attagctggg acgagcaggg taatttgcaa
    33541 tttattaatg cccaggcggc gcgggtctta cgccttgacg cgacggcaag tcagggacgg
    33601 gcaatcactg aactcttaac gttacccgcc gtattgcaac aagcaataaa acaggcacat
    33661 ccgctcaaac acgtagaagc aacctttgaa agccagcacc agtttattga tgcggtgata
    33721 acccttaaac cgataataga aacgcaggga accagcttta ttttgttgct ccatcctgtg
    33781 gaacagatgc ggcagttaat gaccagtcaa ttaggaaaag tcagccatac tttcgctcat
    33841 atgccacaag aggatccgca aacccgccgt ttgattcatt ttggtcgcca ggcggcgcgc
    33901 agtagctttc ctgtcctgct ttgtggagaa gagggcgtgg gcaaggcact gctaagtcag
    33961 gcaattcata atgaaagtga gcgtgcagca gggccttata tcgccgtcaa ttgtgagtta
    34021 tatggtgatg cggcgctggc ggaagaattt attggcggcg atcgcacgga caatgaaaat
    34081 ggtcgtttga gtcgactgga actggcgcac ggcggcacgc tgtttcttga aaagattgaa
    34141 tatctggcgg tggagttaca gtctgctttg cttcaggtta tcaagcaggg tgttatcacg
    34201 cgactggatg cgcggcgttt aatacccatt gatgtcaaag tgattgccac aacgaccgcg
    34261 gacctggcaa tgctggtgga acaaaatcgt tttagtcgcc agctgtatta cgcgctgcat
    34321 gcatttgaaa ttaccatccc gcctctgcgt atgcggcgtg gcagcattcc ggcgctggtg
    34381 aataacaaat tacacagtct tgaaaaacgc ttctctacgc ggctgaaaat tgatgacgat
    34441 gccctcgctc gcctggtttc ctgtgcatgg cctggcaacg attttgaact ttacagcgtc
    34501 atcgagaatc ttgctctgag tagtgataac gggcgcattc gcgtcagtga tttacctgaa
    34561 catctcttta ccgagcaggc gacagatgat gtcagcgcca ctcgcctttc caccagtctg
    34621 tcatttgcgg aagttgaaaa agaggcaatt attaacgcag cccaggtcac aggcggtcgc
    34681 attcaggaaa tgtcggcttt acttggaatc ggccgcacta cgctgtggcg gaaaatgaag
    34741 caacatggca ttgatgcagg gcagtttaag cgccgtggat agcgggagag tatgtacttt
    34801 cccgctatga attgctaatt aatagcgacc atgactgccg tgatttggtc caccatttga
    34861 aggtggcatt ggcttaccgg agggcgcagg accacgaggg ccatcgctgt ggcggtgatg
    34921 atggtgggga acacaagcac ttaacaggct cgcaaagaca attgctaaca ggctgaaggt
    34981 aaatgttttt ttcatgacaa actcttgaac ggttaatgca ctgtgattgt tatcacataa
    35041 aaggcacatt gttaatattg ccaggagtct gattatgatt ttcgataact cagaattgaa
    35101 agaagccgcg tagcaggcg
//
LOCUS       DAZFLD010000017        34878 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.017, whole genome shotgun sequence.
ACCESSION   DAZFLD010000017 DAZFLD010000000
VERSION     DAZFLD010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 34878)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34878
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.017"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..746)
                     /gene="chbR"
                     /locus_tag="ACHKBL_000785"
     CDS             complement(<1..746)
                     /gene="chbR"
                     /locus_tag="ACHKBL_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ChbR"
                     /protein_id="HFO5817698.1"
                     /translation="MMQPVINALEIATAREQQLFNGKNFHVFIYNKTESISGLHQHDY
                     YEFTLVLTGRYFQEINGKRVLLERGDFVFIPLGSHHQSFYEFGATRILNVGISKRFFE
                     QHYLPLLPYCFVASQVYRTNNAFLTYVETVISSLNFRETGLEEFVEMVTFYVINRLRH
                     YREEQVIDDIPQWLKSTVEKMHDKEQFSESALENMVTLSAKSQEYLTRATQRYYGKTP
                     MQIINEIRINFAKKQLEMTNYSVTDIAFEAG"
     gene            complement(754..1104)
                     /gene="chbA"
                     /locus_tag="ACHKBL_000786"
     CDS             complement(754..1104)
                     /gene="chbA"
                     /locus_tag="ACHKBL_000786"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIA"
                     /protein_id="HFO5817699.1"
                     /translation="MMDLDNIPDTQTEVEELEEVVMGLIINSGQARSLAYAALKQAKQ
                     GDFAAAKTMMDQSRMALNEAHLVQTKLIEGDAGEGKMKVSLVLVHAQDHLMTSMLARE
                     LITELIELHEKLKA"
     gene            complement(1155..2513)
                     /gene="chbC"
                     /locus_tag="ACHKBL_000787"
     CDS             complement(1155..2513)
                     /gene="chbC"
                     /locus_tag="ACHKBL_000787"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIC"
                     /protein_id="HFO5817700.1"
                     /translation="MSNVIASLEKVLLPFAVKIGKQPHVNAIKNGFIRLMPLTLAGAM
                     FVLINNVFLSFGEGSFFYSLGIRLDASTIETLNGLKGIGGNVYNGTLGIMSLMAPFFI
                     GMALAEERKVDALAAGLLSVAAFMTVTPYSVGEAYAVGANWLGGANIISGIIIGLVVA
                     EMFTFIVRRNWVIKLPDSVPASVSRSFSALIPGFIILSVMGIIAWTLNTWGTNFHQII
                     MDTISTPLASLGSVVGWAYVIFVPLLWFFGIHGALALTALDNGIMTPWALENIATYQQ
                     YGSVEAALAAGKTFHIWAKPMLDSFIFLGGSGATLGLILAIFIASRRADYRQVAKLAL
                     PSGIFQINEPILFGLPIIMNPVMFIPFVLVQPILAAITLAAYYMGIIPPVTNIAPWTM
                     PTGLGAFFNTNGSVAALLVALFNLGIATLIYLPFVVVANKAQNAIDKEESEEDIANAL
                     KF"
     gene            complement(2598..2918)
                     /gene="chbB"
                     /locus_tag="ACHKBL_000788"
     CDS             complement(2598..2918)
                     /gene="chbB"
                     /locus_tag="ACHKBL_000788"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIB"
                     /protein_id="HFO5817701.1"
                     /translation="MEKKHIYLFCSAGMSTSLLVSKMRAQAEKYEVPVIIEAFPETLA
                     GEKGQNADVVLLGPQIAYMLPEIQRLLPNKPVEVIDSLLYGKVDGLGVLKAAVAAIKK
                     AAAN"
     gene            complement(3217..3555)
                     /gene="osmE"
                     /locus_tag="ACHKBL_000789"
     CDS             complement(3217..3555)
                     /gene="osmE"
                     /locus_tag="ACHKBL_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmE"
                     /protein_id="HFO5817702.1"
                     /translation="MNKNMAGILSAAAVLTMLAGCTAYDRTKDQFVQPVVKDVKKGMS
                     RAQVAQIAGKPSSEVSMIHARGTCQTYILGQRDGKAETYFVALDDTGHVINSGYQTCA
                     EYDTDPQAAK"
     gene            3757..4584
                     /gene="nadE"
                     /locus_tag="ACHKBL_000790"
     CDS             3757..4584
                     /gene="nadE"
                     /locus_tag="ACHKBL_000790"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HFO5817703.1"
                     /translation="MTLQQQIIKALGAKPQINAEEEIRRSVDFLKSYLQTYPFIKSLV
                     LGISGGQDSTLAGKLCQMAINELRQETGNESLQFIAVRLPYGVQADEQDCQDAIAFIQ
                     PDRVLTVNIKGAVLASEQALREAGIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGT
                     DHAAEAITGFFTKYGDGGTDINPLYRLNKRQGKQLLAALGCPEHLYKKAPTADLEDDR
                     PSLPDEVALGVTYDNIDDYLEGKNVPEQVARTIENWYLKTEHKRRPPITVFDDFWKK"
     gene            4814..5701
                     /gene="cho"
                     /locus_tag="ACHKBL_000791"
     CDS             4814..5701
                     /gene="cho"
                     /locus_tag="ACHKBL_000791"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease Cho"
                     /protein_id="HFO5817704.1"
                     /translation="MVRRLTSPRLEFEAAAIYEYPEHLRSFLNDLPTRPGVYLFHGES
                     DTMPLYIGKSINIRSRVLSHLRTPDEAAMLRQSRRISWICTAGEIGALLLEARLIKEQ
                     QPLFNKRLRRNRQLCALQLNEKRVDVVYAKEVDFSRAPNLFGLFANRRAALQALQTIA
                     DEQKLCYGLLGLEPLSRGRACFRSALKRCAGACCGKESHDDHALRLRQSLERLRVVCW
                     PWKGAVALKEQHPEMTQYHIIQNWLWLGAVNSLEEATMLIRTPAGFDHDGYKILCKPL
                     LSGNYEITELDPANDQRAS"
     gene            complement(5661..6236)
                     /gene="ves"
                     /locus_tag="ACHKBL_000792"
     CDS             complement(5661..6236)
                     /gene="ves"
                     /locus_tag="ACHKBL_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="environmental stress-induced protein Ves"
                     /protein_id="HFO5817705.1"
                     /translation="MEYFDMRKMSVNLWRNAAGETREICTFPPAKRDFYWRASIASIA
                     ANGEFSLFPGMERIVTLLEGGEMFLESTDRFNHTLKPLQPFAFAADQVVKAKLTAGQM
                     SMDFNIMTRLDVCKAKVRIAERTFTTFGSRGGVVFVINGAWQLGDKLLTTDQGACWFD
                     GRHTLRLLQPQGKLLFSEINWLAGHSPDQVQ"
     gene            complement(6439..6924)
                     /gene="spy"
                     /locus_tag="ACHKBL_000793"
     CDS             complement(6439..6924)
                     /gene="spy"
                     /locus_tag="ACHKBL_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-independent periplasmic protein-refolding
                     chaperone Spy"
                     /protein_id="HFO5817706.1"
                     /translation="MRKLTALFVASTLALGAANLAHAADTTTSAPADAKPMMHHKGKF
                     GPHQDMMFKDLNLTDAQKQQIREIMKGQRDQMKRPPLEERRAMHDIIASDTFDKAKAE
                     AQIAKMEEQRKANMLAHMETQNKIYNILTPEQKKQFNANFEKRLTERPAAKGKMPATA
                     E"
     gene            complement(7254..8222)
                     /gene="astE"
                     /locus_tag="ACHKBL_000794"
     CDS             complement(7254..8222)
                     /gene="astE"
                     /locus_tag="ACHKBL_000794"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0009017 - succinylglutamate desuccinylase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HFO5817707.1"
                     /translation="MDNFLALTLTGKKTVITEREINGVRWRWLGDGVLELTPLTPPQG
                     ALVISAGIHGNETAPVEMLDALLGAISHGEIPLRWRLLVILGNPPALKQGKRYCHSDM
                     NRMFGGRWQLFAESGETCRARELEQCLEDFYDQGKESVRWHLDLHTAIRGSLHPRFGV
                     LPQRDIPWDEKFLTWLGAAGLEALVFHQEPGGTFTHFSARHFGALACTLELGKALPFG
                     QNDLRQFAVTASAIAALLSGESVGIVRTPPLRYRVVSQITRHSPSFEMHMASDTLNFM
                     PFEKGTLLAQDGEERFTVTHDVEYVLFPNPLVALGLRAGLMLEKIS"
     gene            complement(8215..9558)
                     /gene="astB"
                     /locus_tag="ACHKBL_000795"
     CDS             complement(8215..9558)
                     /gene="astB"
                     /locus_tag="ACHKBL_000795"
                     /EC_number="3.5.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009015 - N-succinylarginine dihydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
                     /protein_id="HFO5817708.1"
                     /translation="MNAWEINFDGLVGLTHHYAGLSFGNEASTRHRFQVSNPRLAAKQ
                     GLLKMKALADAGFPQAVIPPHERPFIPVLRQLGFSGSDEQVLEKVARQAPHWLSSVSS
                     ASPMWVANAATIAPSADTLDGKVHLTVANLNNKFHRSLEAPVTESLLKAIFNDEEKFS
                     VHSALPQVALLGDEGAANHNRLGGHYGEPGMQLFVYGREEGNDTRPSRYPARQTREAS
                     EAVARLNQVNPQQVIFAQQNPDVIDQGVFHNDVIAVSNRQVLFCHQQAFARQSQLLAS
                     LRARVNGFMAIEVPATQVSVSDAVSTYLFNSQLLSRDDGSMMLVLPQECREHAGVWGY
                     LNELLAADNPISELKVFDLRESMANGGGPACLRLRVVLTEEERRAVNPAVMMNDTLFN
                     ALNDWVDRYYRDRLTAADLADPQLLREGREALDVLSQLLNLGSVYPFQREGGGNG"
     gene            complement(9555..11033)
                     /gene="astD"
                     /locus_tag="ACHKBL_000796"
     CDS             complement(9555..11033)
                     /gene="astD"
                     /locus_tag="ACHKBL_000796"
                     /EC_number="1.2.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043824 - succinylglutamate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
                     /protein_id="HFO5817709.1"
                     /translation="MTLWINGDWVTGQGASRVKRNPVSGEVLWQGNDADAAQVGQACR
                     AARAAFPRWARLSFGDRQVRVERFAGLLESNKAELTAIIARETGKPRWEAATEVMAMI
                     NKIAISIKAYHVRTGEQRSEMPDGAASLRHRPHGVLAVFGPYNFPGHLPNGHIVPALL
                     AGNTIIFKPSELTPWSGEAVMRLWQQAGLPPGVLNLVQGGRETGQALSALEDLDGLLF
                     TGSANTGYQLHRQLSGQPEKILALEMGGNNPLIIDEVADIDAAVHLTIQSAFVTAGQR
                     CTCARRLLLKSGTQGDAFLARLVAVSQRLTPGRWDDEPQPFIGGLISEQAAQQVVTAW
                     QELEAMGGRTLLAPRLLQAGTSLLTPGIIEMTGVTGVPDEEVFGPLLRVWRYDNFDEA
                     IRMANNTRFGLSCGLVSPEREKFDQLLLEARAGIVNWNKPLTGAASTAPFGGIGASGN
                     HRPSAWYAADYCAWPMASLESDSLTLPATLNPGLDFSDEVVR"
     gene            complement(11030..12064)
                     /gene="astA"
                     /locus_tag="ACHKBL_000797"
     CDS             complement(11030..12064)
                     /gene="astA"
                     /locus_tag="ACHKBL_000797"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008791 - arginine N-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HFO5817710.1"
                     /translation="MMVIRPVERSDVAALMQLASKTGGGLTSLPANEATLSARIERAI
                     KTWQGELPKSEQGYVFVLEESETGTVAGICAIEVAVGLNDPWYNYRVGTLVHASKELN
                     VYNALPTLFLSNDHTGSSELCTLFLDPDWRKEGNGYLLSKSRFMFMAAFRDKFNDKVV
                     AEMRGVIDEHGYSPFWQSLGKRFFSMDFSRADFLCGTGQKAFIAELMPKHPIYTHFLS
                     QEAQDVIGQVHPQTAPARAVLEKEGFRYRNYIDIFDGGPTLECDIDRVRAIRKSRLVE
                     VAEGQPAQGDFPACLAANENYHHFRVVLVRTDPATERLILTAAQLDALKCHAGDRVRL
                     VRLCAEEKTA"
     gene            complement(12061..13281)
                     /gene="astC"
                     /locus_tag="ACHKBL_000798"
     CDS             complement(12061..13281)
                     /gene="astC"
                     /locus_tag="ACHKBL_000798"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.81"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylornithine/acetylornithine transaminase"
                     /protein_id="HFO5817711.1"
                     /translation="MSQPITRENFDEWMIPVYAPAPFIPVRGEGSRLWDQQGKEYIDF
                     AGGIAVNALGHAHPELREALNEQASKFWHTGNGYTNEPVLRLAKKLIDATFAYRVFFC
                     NSGAEANEAALKLARKFAHDRYGSHKSGIVAFKNAFHGRTLFTVSAGGQPAYSQDFAP
                     LPPDIRHAAYNDINSASALIDDSTCAVIVEPIQGEGGVVPASNAFLQGLRELCDRHNA
                     LLIFDEVQTGVGRTGELYAYMHYGVTPDLLTTAKALGGGFPVGALLATEECASVMTVG
                     THGTTYGGNPLASAVAGKVLDLINTPEMLNGVKQRHDWFVERLNTINHRCGLFSEIRG
                     LGLLIGCVLNADYAGQAKQISQEAAKAGVMVLIAGGNVVRFAPALNVSEEEVTTGLDR
                     FAAACEHFVSGGSS"
     gene            13727..14533
                     /gene="xthA"
                     /locus_tag="ACHKBL_000799"
     CDS             13727..14533
                     /gene="xthA"
                     /locus_tag="ACHKBL_000799"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008311 - double-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HFO5817712.1"
                     /translation="MKFVSFNINGLRARPHQLEAIVEKHQPDVIGLQETKVHDDMFPL
                     EEVAKLGYNVFYHGQKGHYGVALLTKETPIAVRRGFPGDDEEAQRRIIMAEIPSPLGN
                     VTVINGYFPQGESRDHPIKFPAKAQFYQNLQNYLETELKRENPVLIMGDMNISPGDLD
                     IGIGEENRKRWLRTGKCSFLPEEREWMERLMSWGLVDTFRHANPQTANRFSWFDYRSK
                     GFDDNRGLRIDLLLASQPLAECCVETGIDYEIRSMEKPSDHAPVWATFRR"
     gene            14700..15410
                     /gene="ydjX"
                     /locus_tag="ACHKBL_000800"
     CDS             14700..15410
                     /gene="ydjX"
                     /locus_tag="ACHKBL_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HFO5817713.1"
                     /translation="MNAERKFLFACLIFALVIYAFHAFGLFELLTDLPHLLTLIRQSG
                     LFGYSLYILLFIIATLFLLPGSILVIAGGIVFGPLLGTLLSLIAATLASSCSFLLARW
                     LGRDLLLKYVGHSHTFQAIEKGIARNGIDFLILTRLIPLFPYNIQNYAYGLTTIAFWP
                     YTLISALTTLPGIVIYTVMASDLANEGITLRFILQLCLAGLALFILVQLAKLYARHKH
                     VDLSASRRSPLSHPKNEG"
     gene            15415..16092
                     /gene="ydjY"
                     /locus_tag="ACHKBL_000801"
     CDS             15415..16092
                     /gene="ydjY"
                     /locus_tag="ACHKBL_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YdjY"
                     /protein_id="HFO5817714.1"
                     /translation="MLQHYSVSWKKGLAALCLLAVAGLSGCDQKENAAAKVEYDGLSN
                     SQPLRVDANNHTVTMLVQINGRFLTDDTRHGIVFKDGSNGHKSLFMGYATPKAFYEAL
                     KEAGGTPGENMTMDNKETTHVTGSKLDISVNWQGAAKAYSFDEVIVDSNGKKLDMRFG
                     GNLTAAEEKKTGCLVCLDSCPVGIVSNATYTYGAVEKRGEVKFKGNASVLPADNTLAT
                     VTFKITE"
     gene            16106..16813
                     /gene="ydjZ"
                     /locus_tag="ACHKBL_000802"
     CDS             16106..16813
                     /gene="ydjZ"
                     /locus_tag="ACHKBL_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HFO5817715.1"
                     /translation="MMKMQSRKIWYYRITLIILLFAMLLAWALLPGVHEFINRSVAAF
                     AAVDQQGIERFIQSYGALAAVVSFLLMILQAIAAPLPAFLITFANASLFGAFWGGLLS
                     WTSSMAGAALCFFIARVMGREVVEKLTGKTVLNSMDGFFTRYGKHTILVCRLLPFVPF
                     DPVSYAAGLTSIRFRSFFIATGLGQLPATIVYSWAGSMLTGGTFWFVTGLFILFALTV
                     VIFMAKKIWLERQKRNA"
     gene            16813..17361
                     /gene="ynjA"
                     /locus_tag="ACHKBL_000803"
     CDS             16813..17361
                     /gene="ynjA"
                     /locus_tag="ACHKBL_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFO5817716.1"
                     /translation="MGLPPLSKIPFILRPQAWLHRRHYGEVLSPIRWWGRIPFIFYLV
                     SIFVGWLERKRSPLDPVVRSLVSARIAQMCLCEFCVDITSMKVAERTGSTDKLLAVAD
                     WRQSPLFSDEERLALEYAEAASVTPPTVDDALRARLVTHFDAQALTELTALIGLQNLS
                     ARFNSAMDIPAQGLCRIPEKRS"
     gene            17371..18537
                     /gene="ynjB"
                     /locus_tag="ACHKBL_000804"
     CDS             17371..18537
                     /gene="ynjB"
                     /locus_tag="ACHKBL_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFO5817717.1"
                     /translation="MRHCVWLLGLLSLFSLATHASDWQEIKNDAKGQTVWFNAWGGDT
                     AINRYLDWVSGEMKTHYDINLKIVHLADAADAVKRIQTEAAAGRKTGGSVDLLWVNGE
                     NFRTLKEANLLQTDWAETLPNWRYVDTQLPVREDFSVPTEGAESPWGGAQLTFIARRD
                     VTPQPPQTPQALLEFAKANPGTVTYPRPPDFTGTAFLEQLLIMLTPDPAALKEAPDDA
                     TFARVTAPLWQFLDALHPYLWREGKDFPPSPARMDALLKANTLRLSLTFNPAHAQQKI
                     ASGDLPASSYSFGFSEGMIGNVHFVTIPANANASAAAKVVANFLLSPDAQLRKADPAV
                     WGDPSVLDPQKLPDGQRESLQSRMPQDLPPVLAEPHAGWVNALEQEWLHRYGTH"
     gene            18510..20045
                     /gene="ynjC"
                     /locus_tag="ACHKBL_000805"
     CDS             18510..20045
                     /gene="ynjC"
                     /locus_tag="ACHKBL_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFO5817718.1"
                     /translation="MATPLRYALIFLLWAMMAVIYAPLIPAVLTLISPALSLTHWQAL
                     FADPQLPQALLATLVSTTIAAVGALLIALLVIVALWPGPKWQRMCARLPWLLAIPHVA
                     FATSALLLFADGGLLYDYFPYFTPPMDKLGIGLGLTLAVKESAFLLWILAAVLSEKRL
                     LQQLIVLDSLGYSRWQCLNWLLLPSVAPALAMAMLAIVAWSLSVVDVAIILGPGNPPT
                     LAVISWQWLTQGDADQQAKGALASLLLMLLLAAYVLLGYLLWRSWRRTIPRVDGVRKP
                     ATPLLPGITLASFLPLTGVLCVVLLAILADQSTINSEALINSLTMGLTATFIALILIL
                     AWLEWGSSRRHFWLWLPILLPALPLVAGQYTLALWLNLDGSWTAVVWGYLLWVMPWML
                     FILQPAWQRIDSRLILIAQTLGWSRSKIFFYVKCPLMLRPTLIAFAVGFSVSIAQYMP
                     TLWLGAGRFPTLTTEAVALSSGGSNGILAAQALWQLLLPLIIFALTALVAKWVGYVRQ
                     GLR"
     gene            20045..20698
                     /gene="ynjD"
                     /locus_tag="ACHKBL_000806"
     CDS             20045..20698
                     /gene="ynjD"
                     /locus_tag="ACHKBL_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFO5817719.1"
                     /translation="MLCVKNVSLRLPESRLLTNVNFTVDKGDIVTLMGPSGCGKSTLF
                     SWMIGGLAGQFSCTGELWLNEQRIDMLPTAQRQIGILFQDALLFDQFSVGQNLLLALP
                     ATLKGNARRNAVNDALERSGLDGAFHQDPATLSGGQRARVALLRALLAQPKALLLDEP
                     FSRLDVALRDNFRQWVFSEVRAQAIPVVQVTHDLQDVPADSSVLDMAQWSENYNKLR"
     gene            20765..22072
                     /gene="ynjE"
                     /locus_tag="ACHKBL_000807"
     CDS             20765..22072
                     /gene="ynjE"
                     /locus_tag="ACHKBL_000807"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YnjE"
                     /protein_id="HFO5817720.1"
                     /translation="MKRVSQMTALAMALGLACASSWAAELAKPLTLDQLQQQNGKAID
                     TRPSAFYNGWPQTLNGPSGHEPAALNLSANWLDKMSTEQLNEWIKQHNLKADAPVALY
                     GNDKDVDAVKKRLQKAGFTHISILSDALSEPSRLQKLPHFEQLVYPQWLHDLQQGKEV
                     TAKPAGDWKVIEAAWGAPKLYLISHIPGADYIDTNEVESEPLWNKVSDEQLKAMLAKH
                     GIRHDTTVILYGRDVYAAARVAQIMLYAGVKDVRLLDGGWQTWSDAGLPVERGTPPKV
                     KAEPDFGVKIPAQPQLMLDIEQARGLLHRQDASLVSIRSWPEFIGTTSGYSYIKPKGE
                     IAGARWGHAGSDSTHMEDFHNPDGTMRSADDITAMWKAWNIKPDQQVSFYCGTGWRAS
                     ETFMYARAMGWNNVSVYDGGWYEWSSDPKNPVATGERGPDSSK"
     gene            complement(22081..22701)
                     /gene="ynjF"
                     /locus_tag="ACHKBL_000808"
     CDS             complement(22081..22701)
                     /gene="ynjF"
                     /locus_tag="ACHKBL_000808"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFO5817721.1"
                     /translation="MLDRHLHPRIKPLLHQCVKVLDKPGITPDGLTLVGFAIGVLALP
                     FLALGWYLAALIVILLNRLLDGLDGALARRRGLTDAGGFLDISLDFLFYALVPFGFIL
                     AAPEQNALAGGWLLFAFIGTGSSFLAFAALAAKHQIDNPGYAHKSFYYLGGLTEGSET
                     ILLFVLSCLFPACFPWFAWVFGALCWMTTFTRVWSGYLTLKSLQRQ"
     gene            22788..23195
                     /gene="nudG"
                     /locus_tag="ACHKBL_000809"
     CDS             22788..23195
                     /gene="nudG"
                     /locus_tag="ACHKBL_000809"
                     /EC_number="3.6.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP pyrophosphohydrolase"
                     /protein_id="HFO5817722.1"
                     /translation="MKMIEVVAAIIERDGKILLAQRPAQSDQAGLWEFAGGKVEPDES
                     QRQALVRELNEELGIEATVGDYVASHQREVSGRIIHLHAWHVPDFHGTLQAHEHQALV
                     WCSPEEALQYPLAPADIPLLEAFMALRAARPAD"
     gene            complement(23161..23433)
                     /gene="ynjH"
                     /locus_tag="ACHKBL_000810"
     CDS             complement(23161..23433)
                     /gene="ynjH"
                     /locus_tag="ACHKBL_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1496 domain-containing protein"
                     /protein_id="HFO5817723.1"
                     /translation="MSRALFAVVLAFPIIALANPHYRPDVEVNVPPEVFSSGGQSAQP
                     CTQCCVYQDQNYSEGAVIKAEGILLQCQQDDKNLSTNPLVWRRVKP"
     gene            23669..25012
                     /gene="gdhA"
                     /locus_tag="ACHKBL_000811"
     CDS             23669..25012
                     /gene="gdhA"
                     /locus_tag="ACHKBL_000811"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HFO5817724.1"
                     /translation="MDQTYSLESFLNHVQKRDPNQTEFAQAVREVMTTLWPFLEQNPK
                     YRQMSLLERLVEPERVIQFRVVWVDDRNQVQVNRAWRVQFSSAIGPYKGGMRFHPSVN
                     LSILKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKSEGEVMRFCQALMTELYRHLGA
                     DTDVPAGDIGVGGREVGFMAGMMKKLSNNTACVFTGKGLSFGGSLIRPEATGYGLVYF
                     TEAMLKRHGMGFEGMRVSVSGSGNVAQYAIEKAMEFGARVITASDSSGTVVDESGFTK
                     EKLARLIEIKASRDGRVADYAKEFGLVYLEGQQPWSVPVDIALPCATQNELDVDAAHQ
                     LIANGVKAVAEGANMPTTIEATELFQQAGVLFAPGKAANAGGVATSGLEMAQNAARLG
                     WKAEKVDARLHHIMLDIHHACVEHGGEGEQTNYVQGANIAGFVKVADAMLSQGVI"
     gene            complement(25089..27044)
                     /gene="topB"
                     /locus_tag="ACHKBL_000812"
     CDS             complement(25089..27044)
                     /gene="topB"
                     /locus_tag="ACHKBL_000812"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFO5817725.1"
                     /translation="MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGH
                     LLEQAQPDAYDSRYARWNLADLPIVPEKWQLQPRPSVTKQLNVIKRFLHEASEIVHAG
                     DPDREGQLLVDEVLDYLQLAPEKRQQVQRCLINDLNPQAVERAIDRLRSNSEFVPLCV
                     SALARARADWLYGINMTRAYTILGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVA
                     KDFFEVKAHIVTPADERFTAIWQPSEACEPYQDEEGRLLHRPLAEHVVNRISGQPAIV
                     TSYNDKRESESAPLPFSLSALQIEAAKRFGLSAQNVLDICQKLYETHKLITYPRSDCR
                     YLPEEHFAGRHAVMNAISVHAPDLLPQPVVDPDIRNRCWDDKKVDAHHAIIPTARSSA
                     INLTENEAKVYNLIARQYLMQFCPDAVFRKCVIELDIAKGKFVAKARFLAEAGWRTLL
                     GSKERDEENDGTPLPVVAKGDELLCEKGEVVERQTQPPRHFTDATLLSAMTGIARFVQ
                     DKDLKKILRATDGLGTEATRAGIIELLFKRGFLTKKGRYIHSTDAGKALFHSLPEMAT
                     RPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLYQLIDQAKRTSVRQFRGIMAPGGGE
                     GKKKDSPRKRAPKKSPPSEEAGNGVII"
     gene            complement(27049..28092)
                     /gene="selD"
                     /locus_tag="ACHKBL_000813"
     CDS             complement(27049..28092)
                     /gene="selD"
                     /locus_tag="ACHKBL_000813"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HFO5817726.1"
                     /translation="MSENSIRLTQYSHGAGCGCKISPKVLETILHSEQAKFVDPNLLV
                     GNETRDDAAVYDLGNGTSVISTTDFFMPIVDNPFDFGRIAATNAISDIFAMGGKPIMA
                     IAILGWPINKLSPEIAREVTEGGRHACRQAGIALAGGHSIDAPEPIFGLAVTGIVPTE
                     RVKKNSTAQAGCKLFLTKPLGIGVLTTAEKKSLLKPEHQGLATEVMCRMNIAGASFAN
                     IEGVKAMTDVTGFGLLGHLSEMCQGAGVQARVDYDAIPKLPGVEEYIKLGAVPGGTER
                     NFASYGHLMGEMPREVRELLCDPQTSGGLLLAVMPEAENEVKATAAEFGIELTAIGEL
                     VPARGGRAMVEIR"
     gene            complement(28209..28760)
                     /gene="ydjA"
                     /locus_tag="ACHKBL_000814"
     CDS             complement(28209..28760)
                     /gene="ydjA"
                     /locus_tag="ACHKBL_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HFO5817727.1"
                     /translation="MDALELLINRRSASRLAEPAPTGEQLQNILRAAMRAPDHKSMQP
                     WHFFVIEGEGRERFSAVLEQGAIAAGSDNKAIDKARNAPFRAPLIITVVAKCEENHKV
                     PRWEQEMSAGCAVMAMQMAAVAQGFGGIWRSGALTESPVVREAFGCREQDKIVGFLYL
                     GTPQLKASTSINVPDPTPFVTYF"
     gene            28921..30777
                     /gene="sppA"
                     /locus_tag="ACHKBL_000815"
     CDS             28921..30777
                     /gene="sppA"
                     /locus_tag="ACHKBL_000815"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HFO5817728.1"
                     /translation="MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQ
                     VSGGDSKETASRGALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVN
                     TIRQAKDDRNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQY
                     YLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRD
                     DMSPAAREADSRWIGELWQNYLNTVAANRQIPAQQVFPGAQGLLEGLTKTGGDTAKYA
                     LENKLVDALASSAEIEKTLTKEFGWSKTDKNYRAISYYDYALKTPADTGDSIGVVFAN
                     GAIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAA
                     RAAGKPVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGV
                     HTDGVSTSPLADVSITRALPPEAQQMMQLSIENGYKRFITLVADARHSTPEQIDKIAQ
                     GHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKVKQWHLEYYVDEPTFFDKVMDNMSG
                     SVRAMLPDAFQAMLPAPLASVASTVKSESDKLAAFNDPQNRYAFCLTCANVR"
     gene            30942..31958
                     /gene="ansA"
                     /locus_tag="ACHKBL_000816"
     CDS             30942..31958
                     /gene="ansA"
                     /locus_tag="ACHKBL_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HFO5817729.1"
                     /translation="MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRP
                     EMPDFTIHEYTPLMDSSDMTPEDWQHIAEDIKAHYDDYDGFVILHGTDTMAYTASALS
                     FMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAANYPINEVTLFFNNRLYRGNR
                     TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIY
                     PGISADVVRNFLRQPVKALILRSYGVGNAPQNKAFLQELQEASDRGIVVVNLTQCMSG
                     KVNMGGYATGNALAHAGVIGGADMTVEATLTKLHYLLSQELDTETIRKAMSQNLRGEL
                     TPDD"
     gene            31969..32610
                     /gene="pncA"
                     /locus_tag="ACHKBL_000817"
     CDS             31969..32610
                     /gene="pncA"
                     /locus_tag="ACHKBL_000817"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001587135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HFO5817730.1"
                     /translation="MHPRALLLVDLQNDFCAGGALAVPEGDSTVDVANRLIDWCQSRG
                     EAVIASQDWHPANHGSFASQHGVEPYTPGQLDGLPQTFWPDHCVQNSEGAQLHPLLNQ
                     KAIAAVFHKGENPLVDSYSAFFDNGRRQKTTLDDWLRDHEIDELIIMGLATDYCVKFT
                     VLDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQE"
     gene            complement(32771..33043)
                     /gene="yeaC"
                     /locus_tag="ACHKBL_000818"
     CDS             complement(32771..33043)
                     /gene="yeaC"
                     /locus_tag="ACHKBL_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaC family protein"
                     /protein_id="HFO5817731.1"
                     /translation="MNLDDIINSMTPEVYQRLSTAVELGKWPDGVTLTEEQKENCLQL
                     VMLWQARNNTEAQHMTIDTNGQMVMKSKQQLKEDFGISAKPIAMFK"
     gene            complement(33085..33498)
                     /gene="msrB"
                     /locus_tag="ACHKBL_000819"
     CDS             complement(33085..33498)
                     /gene="msrB"
                     /locus_tag="ACHKBL_000819"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HFO5817732.1"
                     /translation="MANKPSAEELKKNLSEMQFYVTQNHGTEPPFTGRLLHNKRDGVY
                     HCLICDAPLFHSQTKYDSGCGWPSFYEPVSEESIRYIKDLSHGMQRIEIRCGNCDAHL
                     GHVFPDGPQPTGERYCVNSASLRFTDGENGEEING"
     gene            33840..34835
                     /gene="gapA"
                     /locus_tag="ACHKBL_000820"
     CDS             33840..34835
                     /gene="gapA"
                     /locus_tag="ACHKBL_000820"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006322611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFO5817733.1"
                     /translation="MTIKVGINGFGRIGRIVFRAAQKRSDIEIVAINDLLDADYMAYM
                     LKYDSTHGRFDGTVEVKDGHLIVNGKKIRVTAERDPANLKWDEVGVDVVAEATGLFLT
                     DETARKHITAGAKKVVMTGPSKDNTPMFVKGANFDKYAGQDIVSNASCTTNCLAPLAK
                     VINDNFGIIEGLMTTVHATTATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPELNGKLTGMAFRVPTPNVSVVDLTVRLEKAATYEQIKAAVKAAAEGEMKGVLGYTE
                     DDVVSTDFNGEVCTSVFDAKAGIALNDNFVKLVSWYDNETGYSNKVLDLIAHISK"
BASE COUNT         8387 a         9224 c         8748 g         8519 t
ORIGIN      
        1 ccggcctcaa acgcaatatc cgtcactgaa tagttggtca tttccagttg ttttttggca
       61 aaattaatac ggatttcatt aataatctgc attggcgttt taccatagta tcgttgagtc
      121 gctcgcgtca aatattcctg tgatttggct gacaacgtca ccatattctc cagcgccgat
      181 tcactaaact gctctttatc atgcatcttt tctaccgtac ttttcagcca ctgcggtata
      241 tcatcaatca cctgttcttc gcggtaatga cgtaaacggt taatgacata aaaagtaacc
      301 atctcaacaa actcttccag ccctgtttcg cggaaattca atgaagaaat cactgtttcc
      361 acataggtga gaaaagcgtt attggtccgg tatacctgcg aagcgacaaa gcaataaggc
      421 aacaacggca ggtaatgctg ctcaaaaaag cgtttactga tcccaacgtt caatatacgc
      481 gtggcaccaa attcataaaa actttgatgg tgcgaaccta acggaataaa aacaaaatcg
      541 ccccgttcca gtaacacgcg cttaccgtta atctcctgga aataacgccc ggttaatacc
      601 agagtaaatt cataatagtc gtgctgatgc agtccgctga tactctcagt tttattgtag
      661 ataaacacat ggaaattttt gccattaaac aactgctgtt ctcgggcagt ggcaatttcc
      721 agcgcgttaa tcactggctg catcatcgac tccttatgcc ttcagttttt catgaagctc
      781 aattaattca gtaatcagtt cacgcgcaag catggacgtc attaaatgat cctgagcgtg
      841 gaccagcacc agactcactt tcatcttacc ttcgcccgca tcgccttcaa tcagtttcgt
      901 ctgtaccaga tgcgcttcat tcaatgccat ccgtgactga tccatcatgg tttttgctgc
      961 ggcaaaatcg ccctgcttcg cctgtttcag tgccgcatac gccaggctgc gcgcttgtcc
     1021 ggagttgatg ataagcccca tcaccacttc ttccagctct tcaacttccg tttgcgtatc
     1081 gggaatatta tcgagatcca tcatacatcg ttcctctcta ttcttaccgg cacgattacc
     1141 cgtaccggca tcgattaaaa tttcagggcg ttagcgatat cttcttcgct ctcttcttta
     1201 tcaatcgcgt tttgtgcttt gttagccacc acaacaaagg gcagataaat taacgttgcg
     1261 ataccaaggt tgaagagtgc gaccagcaat gcggcgacgc taccgttggt gttaaagaag
     1321 gctcccagac cggttggcat ggtccacggt gcaatattgg tcaccggagg aataatgccc
     1381 atgtagtacg ctgcgagggt gattgccgcc agaatcggtt gtaccagtac aaacgggata
     1441 aacatcaccg ggttcatgat aattggcaga ccaaacagaa tcggttcgtt aatctggaag
     1501 atgccggacg gcagcgccag ttttgccacc tgacgataat cagcacggcg agaggcgata
     1561 aagatagcca ggatcagacc taaagtcgca ccactgcccc caaggaaaat aaaggagtcc
     1621 agcatcggct tggcccagat atggaaggtc ttaccggctg ccagcgccgc ttcgacggaa
     1681 ccatattgct gataggtcgc gatattttcc agcgcccaag gcgtcataat gccgttgtcc
     1741 agtgcggtca gcgccagcgc accatgaata ccgaagaacc agagcagtgg aacaaagatc
     1801 acataggccc agcccaccac gctacccaac gatgccagtg gggttgagat ggtatccata
     1861 ataatctgat ggaagttggt gccccaggta ttcaacgtcc aggcaataat ccccatcacg
     1921 gaaagaataa taaagccggg aattaaagcc gagaaggaac gcgatactga agcaggtacg
     1981 ctatcgggca atttaatgac ccaattgcgg cggacaataa aggtaaacat ttctgccacc
     2041 accaggccaa taataatccc ggagatgata ttcgccccac ctaaccagtt tgcgccaacc
     2101 gcataggcct caccgacact atatggggtg acggtcataa atgctgcaac ggataacaac
     2161 ccggccgcca gcgcatcgac tttacgctct tctgccagcg ccatgccaat aaagaacggt
     2221 gccattaaag acattatccc taatgttccg ttatatacgt tgccgccaat acctttcaga
     2281 ccattaagtg tttcaatggt tgaggcatcc aggcgaatac ctaaggaata aaaaaacgac
     2341 ccctccccaa agcttagaaa aacgttgtta attaatacaa acatggcccc cgcaagggtt
     2401 aacggcatta agcgaataaa gccatttttg attgcattaa cgtgtggctg ctttcctatt
     2461 ttaactgcaa aagggaggag taccttttca agcgatgcaa taacattact catagaaaaa
     2521 tacccttaaa aaccgcaatt taaatattgc ggtattgatt tatgaaataa ctctttgacg
     2581 ggaaaattta aaataaatta atttgctgcg gcttttttaa tcgctgcaac cgcagcctta
     2641 agcacgccta aaccatcgac tttgccataa agcagcgagt caattacttc gaccggtttg
     2701 ttgggtaaca aacgctggat ttcgggcaac atataagcaa tctgcggccc taataacacg
     2761 acatcggcat tctgaccttt ttcaccagcc agtgtttccg gaaatgcttc aataatgacc
     2821 ggaacttcat atttttctgc ctgtgcgcgc atttttgata ccagtaaaga ggtagacatg
     2881 cccgcagaac aaaacagata aatgtgtttc ttttccataa aactgccctc atcgacgatt
     2941 atctgtcagc cagacactcc gcaagcctta acctgcttcc atgctctggg taacttgcga
     3001 aaccaacatg actttttttg tggctgaaag gagtatacgg tatagacctg agcaatagta
     3061 tttctttgac ctgataaatt gtctctcctt gacctgcggt tatgaccctc ttcacatttc
     3121 gggcaaataa ttcgcgaaga taattaagaa ctcaggccaa aaaaaaccgg cgcaatggcc
     3181 ggtttccgtt gtaactcaag ctttcaggcg aagtgattac ttcgcagcct gtggatcagt
     3241 gtcgtattca gcacaggtct gataaccgga gttgatgaca tgtccggtat catctaacgc
     3301 gacaaagtag gtttctgctt taccatcacg ttgacccagg atgtaggtct ggcaagtacc
     3361 acgagcatgg atcatgctca cttcagacga aggtttaccc gcaatttgtg caacctgcgc
     3421 ccggctcatg ccttttttga cgtctttcac cacaggctgt acaaactggt ctttggtacg
     3481 atcataagcc gtacaacccg ccagcatggt taataccgcc gctgcactca gaattcctgc
     3541 catattcttg ttcatattcc gtcctcttgt ttatcagcgt gttagataag cctggaatac
     3601 attgggcgct ttttcaagcc cgtgaacgaa acggctccgc tttcagagga ttcctgtatg
     3661 acgttttaac caccattcag cccgctgtcg cttgtcgttt cagtagcaac gggttagctt
     3721 taaggaagtt ttgtcttttc tgtctggagg ggttcaatga cattgcaaca acaaataata
     3781 aaggcgctgg gcgcaaaacc gcagattaat gctgaagagg aaattcgtcg tagtgtcgat
     3841 tttctgaaaa gctacctgca aacttatccg ttcattaaat cactggtgct cgggatcagc
     3901 ggcggtcagg actccacgct tgccggaaag ctgtgccaga tggcgattaa tgagctgcgc
     3961 caggaaactg gcaacgaatc actgcaattt attgccgtac gcctgcccta tggtgttcag
     4021 gctgacgaac aagattgcca ggatgccatt gcctttattc aaccggatcg cgtattaacc
     4081 gttaatatca agggcgcggt attggccagc gaacaggcgc tgcgcgaagc gggtattgaa
     4141 ttaagtgatt ttgtccgtgg caatgaaaaa gcgcgtgagc ggatgaaagc acaatatagc
     4201 attgcgggta tgaccagcgg tgtcgtggtg ggcaccgatc atgcagcaga agccattacc
     4261 ggattcttca ctaaatatgg tgacggcggt acggacatta atccactgta tcgtcttaac
     4321 aaacgtcagg gtaaacagtt actggcggca ttaggttgcc cggaacacct ttataagaaa
     4381 gcgccaacgg ccgatctgga agatgatcgc ccttctctac cagatgaagt ggcactcggc
     4441 gtgacctatg acaatatcga cgactatctg gaagggaaaa acgtacctga acaggtcgcc
     4501 agaacaatag agaactggta tctgaaaact gaacataaac gccgtccgcc aattaccgtt
     4561 ttcgatgatt tctggaagaa gtaataattt gcacatattg gtttgtgcga aaaagagtac
     4621 tttgttcacg ccggatgcgg cgtgaacgcc ttattcgccc cacaaaacta tgcaaattca
     4681 atatattgca tgagtcgaat aggcctgaca ggcgtagcac gtctgacggt gtaacctttg
     4741 tcatcaaccc gcctcttttt taatcgctcc ccgcctgtta cactggatag ataaccagca
     4801 ttcggagtca actgtggtac ggcgtttaac ttctccgcgg ctcgaatttg aagctgcggc
     4861 aatttatgaa tatcccgaac atttacgttc attccttaat gacttaccca cccgaccagg
     4921 ggtgtatctg tttcatggcg aaagtgacac catgccgctc tatatcggca aaagcattaa
     4981 cattcgcagc cgcgtccttt ctcatttacg taccccggat gaagccgcca tgctacggca
     5041 atcccgacgc atcagctgga tatgtaccgc cggtgaaatc ggcgctctac tccttgaagc
     5101 gcgattaatc aaagagcagc agccgctgtt taataaacgc ttgcgccgca atcgccagct
     5161 ctgtgccctg caattaaatg aaaagcgcgt cgatgtggtg tatgccaaag aggtggattt
     5221 ttcacgagcc cccaacctgt ttggcctgtt tgccaatagg cgcgcagctt tgcaagcatt
     5281 gcagaccatc gctgatgaac aaaaactttg ttatggtctg ttgggactgg agccgttaag
     5341 tcgcggtcgt gcatgttttc gttcagcgct aaaacgttgc gccggagcat gctgcggtaa
     5401 agagagccat gacgatcatg cgctacgttt gcgccagtct ctggaacgtt tacgagtagt
     5461 ttgttggccg tggaaaggtg cggtggcgct gaaagaacag cacccggaaa tgactcaata
     5521 tcatattatt caaaactggc tgtggctggg ggcggttaat tcgctggaag aagcgacaat
     5581 gttaattcgg acacccgccg ggtttgatca cgacggttat aaaattcttt gtaagccgct
     5641 gctttccggt aactatgaaa ttactgaact tgatccggcg aatgaccagc gagccagttg
     5701 atttcactga acaacagttt accttgcggt tgtaaaagcc gcagggtgtg cctgccatcg
     5761 aaccagcacg cgccctggtc ggtagtgagc agtttatcgc ccaattgcca cgccccgtta
     5821 atgacaaaca ctaccccgcc acgtgagcca aaggtagtaa acgtacgttc ggcaattctg
     5881 actttcgcct tacagacatc caggcgtgtc atgatgttga aatccatcga catctgccct
     5941 gccgtcagtt tcgcttttac cacctggtcc gccgcaaagg caaaaggctg taacggtttt
     6001 aaggtatggt taaagcggtc tgtgctttca aggaacatct cgccgccttc cagcaacgtc
     6061 actatccttt ccatacctgg aaacaaagaa aactccccat tcgccgcgat ggatgcaatg
     6121 ctggcacgcc agtaaaaatc acgttttgcc ggtgggaaag tgcaaatttc gcgcgtttca
     6181 ccggcagcat ttcgccacag attcaccgac attttacgca tatcaaagta ttccatactc
     6241 gctcccggcc cgttcttttc attgttatcg ttcaggtaac cgtggatggt tatcggcaac
     6301 tcgggtgatt tacgttagtg gcgatcaggc tttttttgca tctgtttgag aacaaaaaag
     6361 aaaaccgccg atcctgtcca ccgcattact gcaaggtagt ggacaagacc gacggtctta
     6421 cgttttttgg ctgaaagatt attcagcagt tgcaggcatt ttaccttttg ccgctggacg
     6481 ttctgtcaga cgcttctcaa aattagcatt aaattgcttt ttctgttccg gcgtcaagat
     6541 gttgtaaatc ttgttctggg tttccatgtg cgccagcatg ttagctttgc gctgttcttc
     6601 catttttgcg atctgcgctt cagcttttgc tttatcgaag gtatcgctgg caatgatgtc
     6661 atgcattgcg cggcgttctt ccagcggcgg acgtttcatc tggtcacgct ggcctttcat
     6721 gatttcgcgg atctgctgtt tctgcgcgtc ggtcaggttc aggtctttga acatcatgtc
     6781 ctgatgcgga ccgaacttgc ctttgtggtg catcatcggc ttcgcgtcag ccggtgctga
     6841 ggtagtggtg tctgcggcat gggccaggtt agccgcgcca agagccaggg tagaggcaac
     6901 aaacagtgca gttaatttac gcatattcta tatccttcct ttcagttatt tattacggct
     6961 ttcttaagta gcgtgccgtg ttgacgagat taactttact gactttagcg tcaattaatc
     7021 agagcaacgg taaaacaatg aaagtgtaaa aaacactttt gcgccaatta tggagaaaaa
     7081 aagaaaattt gatggagagt aatgagagaa tattacaaca cgatgatttt gcagagatta
     7141 tgaagaacta taccggatga ctggtgataa ataaagcaaa taaccaggat taatctgtat
     7201 taatttataa gaaagtaact taatacccgc agaatgattt ctgcgggtaa gtattagctt
     7261 attttttcga gcattaatcc cgcgcgtaat cccaacgcta ccaaaggatt agggaataac
     7321 acatactcta catcatgggt tacggtaaaa cgttcctccc cgtcctgcgc cagcaatgtt
     7381 cctttctcaa acggcataaa attcagcgtg tcacttgcca tatgcatttc gaaggacggc
     7441 gagtggcgag taatttgcga aaccacccga taacggagcg gcggcgttct cacgataccg
     7501 acactctcac cagatagcag cgcagcaatt gcgctggcag tgactgcaaa ctggcgaaga
     7561 tcgttttgtc caaagggcaa cgctttgccg agttccagcg tacaggccag cgcgccaaaa
     7621 tgtctggcgc tgaaatgggt aaacgtacca ccaggctcct gatggaacac cagtgcctcc
     7681 agacccgccg cacccagcca cgtcagaaat ttctcgtccc agggaatgtc gcgttgcggt
     7741 aatacaccga accgcggatg caaggagcca cgaattgcgg tatggagatc aaggtgccag
     7801 cgcacagatt ctttgccctg gtcataaaaa tcttccaggc actgctccag ttcgcgcgcc
     7861 cgacaggttt ccccgctttc agcaaatagc tgccaacgac cgccaaacat tcgattcata
     7921 tcgctatggc aataacgttt cccttgcttc agcgcaggag gattcccgag gatcaccagt
     7981 aaccgccaac gtaacgggat ctcaccgtga gatatcgcgc caagcaacgc gtccagcatt
     8041 tccacaggtg ccgtctcatt accgtggatc cccgctgaaa tcaccagcgc gccttgcggt
     8101 ggcgttaatg gcgtcagttc cagcacgcca tcgcccagcc agcgccagcg aacgccgttg
     8161 atttctcgct cggtgataac cgttttttta cccgttaagg tcagagcaag aaaattatcc
     8221 atttccccct ccctcgcgct ggaacggata aaccgaaccg agattcagta attggctcaa
     8281 tacatccagt gcttcccgcc cttcgcgcag caattgcggg tcggccagat cggcagcagt
     8341 aaggcgatcg cggtagtaac gatccaccca gtcattgagc gcattaaaca gcgtatcgtt
     8401 catcatcacc gccggattca ctgcccggcg ttcttcttct gtcaatacca cgcgcaaccg
     8461 caggcacgcc gggcctccgc cattcgccat gctttcacgt aaatcaaaga cttttagttc
     8521 gctaatcggg ttgtcagcgg caaggagttc attgagataa ccccatactc cggcgtgttc
     8581 ccgacactcc tgaggcagca ccaacatcat ggaaccatca tcgcggctca gcagttggct
     8641 gttaaacaga tatgtagaca ccgcatctga cacggaaacc tgagtcgccg gaacttctat
     8701 cgccataaaa ccgttcaccc gcgcacgcag gcttgccagt aactgtgact ggcgagcgaa
     8761 cgcctgttga tggcaaaaca gcacctggcg gttactcacg gcaatcacgt cattatgaaa
     8821 aacgccctgg tcgataacgt ccgggttttg ctgggcgaaa atcacctgtt ggggattcac
     8881 ctgattcagc cttgccaccg cctcgctggc ttcgcgagtc tgccgcgccg gataacggga
     8941 aggccgggta tcattgcctt cttctcgccc gtagacaaaa agttgcatac ccggttcacc
     9001 gtaatgaccg ccgagacgat tgtggtttgc cgccccctca tcaccgagca acgctacctg
     9061 cggcaacgcc gaatggacac taaatttctc ttcgtcgtta aaaatcgctt ttaacaacga
     9121 ttcggtgacg ggggcttcca gcgaacggtg aaatttattg ttcaggttgg caacggtgag
     9181 atgcactttg ccatccagcg tatcggcaga tggcgcgatc gttgccgcat tggctaccca
     9241 cattggcgaa gcggagctga cgctggaaag ccagtgcggt gcctggcggg caaccttttc
     9301 cagtacctgc tcatcgctgc cgctgaatcc caactgacgc agcaccggaa taaacggacg
     9361 ctcgtgcggc gggatcaccg cctgggggaa tcccgcgtcg gcaagggctt tcattttcag
     9421 taagccctgc ttcgccgcca gtcgcgggtt agagacctga aaacggtgac gggtggaggc
     9481 ttcattacca aacgacaggc ccgcgtaatg atgcgtcagc cctaccagcc cgtcgaaatt
     9541 gatttcccag gcgttcatcg caccacctca tcggaaaaat ccagcccggg gttaagcgtt
     9601 gcgggcaatg ttaacgagtc cgactccaga cttgccatcg gccaggcaca ataatctgcg
     9661 gcataccagg cgctggggcg atggttaccg gaagcgccaa tgccgccgaa tggcgcggta
     9721 ctggcagcac cggtaagcgg tttgttccag ttaacaatcc ccgcccgtgc ctccagcaac
     9781 agttgatcga atttttcccg ctcgggggaa accagaccgc aagagaggcc gaagcgagtg
     9841 ttattcgcca ttcgaatcgc ttcatcgaaa ttgtcataac gccagacgcg caataacggc
     9901 ccaaacactt cttcatccgg cacgccagta acgcctgtca tttcaatgat ccccggcgtc
     9961 agcaacgatg tccctgcttg taataagcgc ggcgcaagca gggtccgtcc gcccatcgcc
    10021 tccagttcct gccaggcagt aaccacctgc tgtgcggcct gctcagaaat cagcccgcca
    10081 ataaacggct gcggttcgtc atcccaacgg cccggcgtta accgctggct gacggcaacc
    10141 aggcgagcaa gaaatgcatc gccctgcgtt ccacttttca gcaataaacg gcgggcgcag
    10201 gtgcagcgtt gcccggcagt gacaaacgcc gactgaatgg tcagatggac agcggcatcg
    10261 atatccgcca cctcatcgat aattagcggg ttattaccgc ccatctcaag ggcgagaatt
    10321 ttctccggct gaccggaaag ctggcgatgc agctggtagc cggtattggc gctgccggta
    10381 aacagcaagc cgtcgagatc ctccagcgca ctcagcgcct gacccgtttc acgcccgccc
    10441 tgcaccaggt tcagcacgcc cggcggcaag ccagcctgct gccataaacg cattaccgct
    10501 tcgccactcc acggtgtcag ttcgctgggt ttaaagataa tggtgttacc tgccagcaat
    10561 gccggaacga tatgtccgtt cggtaaatga ccggggaaat tatacggccc aaacaccgcc
    10621 agcacgccgt gcgggcgatg tcgcaggctc gccgcgccgt ccggcatttc actacgctgc
    10681 tcgccggtac gaacgtgata cgccttaatt gatatcgcga ttttattgat catcgccatc
    10741 acttcggttg ccgcttccca gcgcggctta cccgtttctc tggcaataat cgcggttaat
    10801 tcggctttat tgctttccag caatccggca aagcgttcaa ctcttacctg acgatcgcca
    10861 aaggagagac gcgcccagcg cggaaacgcc gcacgggcag cccgacaagc ctgcccgacc
    10921 tgagcggcat cggcatcatt gccttgccat aacacctcgc ccgataccgg attacgcttc
    10981 acacgcgatg cgccctggcc cgtaacccag tcaccattga tccataaagt catgctgttt
    11041 tctcctctgc gcacaggcgc accagacgaa cgcgatcccc ggcgtggcat ttcagggcat
    11101 ccagttgtgc ggcggttaat atcaaacgct cggttgccgg atcggtacgc accagcacca
    11161 cgcggaaatg gtgataattt tcattggcgg ccaggcaggc cgggaaatcg ccctgcgcag
    11221 gctgcccttc tgccacttcc accagccgac ttttgcggat ggcgcgcacg cggtcgatgt
    11281 cacactcaag cgtcggcccg ccgtcaaaga tgtcgatata gttgcggtag cgaaaacctt
    11341 ctttctccag caccgcgcgg gcgggcgcgg tttgcggatg tacctggccg ataacgtcct
    11401 gcgcttcctg ggataaaaag tgggtataga tcggatgttt cggcatcagt tccgcaataa
    11461 atgccttttg cccggtgcca cagagaaaat cggcgcggct aaaatccatc gaaaagaagc
    11521 gtttaccgag gctttgccag aacggcgaat agccatgttc gtcaatcacc ccgcgcattt
    11581 cggcgaccac tttgtcatta aacttgtcgc gaaaggccgc cataaacata aagcgcgatt
    11641 tcgacagcaa atagccgttg ccctctttgc gccagtccgg gtcgagaaac agcgtgcaca
    11701 gctcgctgct gccggtgtga tcgttactga gaaacagcgt cggcaacgcg ttatagacat
    11761 tcagctcttt tgaggcgtga accagcgtgc cgacgcgata gttgtaccag ggatcgttca
    11821 gcccaaccgc cacctcaatg gcgcaaatcc ccgccacagt gcctgtttcg ctctcttcca
    11881 gtacaaacac ataaccctgc tcgcttttgg gcagttcgcc ctgccaggtt ttgatggccc
    11941 tttcgatacg cgctgaaagc gtggcttcat tagcagggag cgacgtcagg ccgccgcccg
    12001 ttttgctggc aagctgcatc agcgccgcaa cgtctgagcg ctcaacggga cggatgacca
    12061 tcatgatgaa cctccgctca caaagtgttc acaagccgcc gcaaaacgat ccagcccggt
    12121 cgtcacctct tcttcgctga cattgagcgc tggcgcaaaa cgcactacgt tgccaccggc
    12181 aatcagcacc attacgcccg ctttcgccgc ttcctgagaa atttgtttcg cttgcccggc
    12241 gtaatcggca ttcagcacac agccaatcag cagacctaat ccgcgaattt cactgaacaa
    12301 accgcagcgg tgattaatgg tattaagacg ctcaacaaac cagtcgtgac gctgtttaac
    12361 gccattaagc atctctggcg tgttgatgag atccagcact ttacctgcca ccgccgaggc
    12421 cagcggatta ccgccataag tggtaccatg tgtgcccacg gtcatcacgc tggcgcattc
    12481 ttcggttgcc aacagagcgc caacggggaa accgccgccc agtgctttgg cggtagttaa
    12541 cagatcgggc gtcacgccgt agtgcatata ggcatacagt tccccggtgc gcccgacgcc
    12601 ggtttgtact tcatcaaaaa tcaacagcgc attgtggcgg tcacacaatt cacgcagacc
    12661 ttgtaaaaat gcgttgcttg ctggcaccac accgccttcc ccctggatgg gttcgacaat
    12721 caccgcacag gtagagtcgt caatcagcgc gctggcagag ttaatatcgt tatatgcggc
    12781 atgacgaata tccggcggca gtggcgcaaa atcctgtgaa taggctggct gaccgcccgc
    12841 actgacggta aacagcgtgc gaccatgaaa cgcattttta aacgccacga taccgctctt
    12901 atggctgccg tagcggtcgt gagcgaattt acgcgccagt tttagcgccg cttcgttggc
    12961 ttccgcaccg gagttacaaa agaagacgcg ataggcaaac gtggcgtcga tcaatttttt
    13021 cgccagtcgc agtaccggct cgttggtgta accgttgccg gtatgccaga acttactcgc
    13081 ctgttcgttc agcgcctcac gcagttccgg atgcgcatgg cccagcgcgt tcaccgcaat
    13141 gccacccgcg aagtcgatat actctttccc ctgctgatcc cacaagcgcg aaccttcgcc
    13201 acgtaccggt ataaacggtg ccggagcgta aacaggtatc atccattcat caaagttttc
    13261 acgcgtaatt ggctgagaca tagcgacctc tacagtaaat aattcgttat ttatatgtta
    13321 ataataagta atgtttgcgc tgtaaatgta gattgcaggg ttcgtgccag ccagtgataa
    13381 aagtgcataa acggcggagg ctaactggaa atcaaggagt tataaccaaa ccatatgcat
    13441 ttaaagtgca tataaagtga atacgtttgc gatgtgggtg aataaaaaga ataaaaaacg
    13501 caatgttatg cagaagtaaa atataattct ggaattgtga tcattgacga aatttactgg
    13561 aaattactgc gccattctga cgcaacgcgc accaaaagcg ggcatttttt gcgccatcgt
    13621 tgacatcatt aacaaccatc gatcaaatca cttaacaaca ggcggtacgc aaagcgaaat
    13681 tctgctacca tccacgcact ctttatctga ataaatggca gcgactatga aatttgtctc
    13741 ttttaatatc aacggcctgc gcgccagacc tcaccagctt gaagccatcg tcgaaaagca
    13801 ccaaccggat gtgattggcc tgcaggagac aaaagttcat gacgatatgt ttccgctcga
    13861 agaggtggcg aagctcggct acaacgtgtt ttatcacgga cagaaaggcc attatggcgt
    13921 ggcgctgctg accaaagaga cgccgattgc cgtgcgtcgc ggctttcccg gtgacgacga
    13981 agaggcgcaa cggcggatta ttatggcgga aatcccctcg ccgctgggca atgtcaccgt
    14041 gatcaacggt tacttcccgc agggtgaaag ccgcgaccat ccgataaaat tcccggcaaa
    14101 ggcgcagttt tatcagaatc tgcaaaacta tctggaaacc gaactcaagc gtgaaaatcc
    14161 ggtgctgatt atgggcgata tgaatatcag tcctggcgac ctggatattg gcattggcga
    14221 agagaaccgt aagcgctggc tgcgtaccgg aaaatgttct ttccttccgg aggaacgcga
    14281 atggatggag agactgatga gctggggatt ggttgatacc ttccgccatg cgaatccgca
    14341 aacagccaat cgtttctcat ggtttgatta ccgctcaaaa ggttttgacg ataaccgtgg
    14401 tctgcgcatc gacctgcttc tcgccagcca accgctggca gaatgttgcg tagaaaccgg
    14461 catcgactat gaaatccgca gcatggaaaa accgtccgat cacgcccccg tctgggcgac
    14521 cttccgccgc taatttagct gttctcctgg ctcaaactgg gtcaggagaa ttaaccttga
    14581 gaaaaatcaa caaactgtca gtaatgattt gttgcctgcc gtcctttgtt ataccgtcac
    14641 tgcgttttta gttgtctgac cacttctcta ttatcaagtt tgatatagga aactccacga
    14701 tgaacgctga gcgtaaattt ctttttgcct gtcttatttt tgcgctggtc atttacgctt
    14761 tccacgcttt cggtttattc gagctgctca ccgatttacc tcacttactg acactcattc
    14821 gccagagcgg attgttcggt tatagcctct atattctgtt attcatcatt gccaccctct
    14881 ttctgttacc aggaagcata ctggtgatcg ctggaggaat agtttttggc ccgctcttag
    14941 ggacactact atcattaatt gccgccacgc tggcctcctc gtgctcattc ctgctggcgc
    15001 gctggctggg acgtgattta ctgctgaaat acgttggcca tagccatacc tttcaggcca
    15061 ttgaaaaagg cattgcgcgt aacggtattg attttcttat tctgacccgc ttaatcccgt
    15121 tgtttcctta caatattcaa aattacgctt acggattaac cacaatcgcc ttctggcctt
    15181 atacccttat ttcggcactc acaaccctgc ccggtattgt tatttatacc gtgatggcaa
    15241 gcgatctcgc caatgaaggc attacgctgc gctttatttt acaactctgt ctggcgggcc
    15301 tggcactgtt tattctcgtc cagctcgcaa aactctacgc ccgacacaaa catgtggatc
    15361 tgtctgcttc gcgccgcagc ccactttctc accccaaaaa tgaaggatag aacgatgttg
    15421 caacattatt cagtgtcatg gaaaaaagga ctggctgcac tctgcttact ggctgttgcc
    15481 gggcttagcg gctgcgatca aaaagagaat gcggctgcaa aagtggaata cgacggactt
    15541 tcgaacagcc aaccgctgcg tgtcgatgcc aataaccata cggtaaccat gctggtgcaa
    15601 attaatggtc gtttcctcac cgacgacact cgtcacggta ttgtgtttaa agatggctcc
    15661 aacggacata aatcgctgtt tatgggttat gcgaccccga aagcctttta tgaagccctg
    15721 aaagaggcag gtggtacacc gggcgaaaac atgacgatgg ataataaaga aacgactcat
    15781 gtcacaggca gcaaactgga tatttcggtc aactggcaag gggcggcaaa agcgtattcc
    15841 ttcgatgaag tgattgttga cagtaatgga aagaaactgg acatgcgctt tggcggtaat
    15901 ttaacggcag cagaagagaa gaaaacgggc tgcctggtgt gtctggatag ctgcccggtc
    15961 ggcatcgtca gcaatgcaac atacacttat ggtgcggttg aaaaacgagg tgaagttaaa
    16021 ttcaaaggca atgcctcggt tctcccggcg gataacacgc tggcaacggt tacctttaaa
    16081 atcaccgaat aaagcaggat aaaggatgat gaagatgcaa tcgcgaaaaa tctggtacta
    16141 ccgtataacc ctcatcatcc tgttgttcgc tatgctgctg gcatgggcgc tgcttcccgg
    16201 cgtccatgag tttatcaatc gcagcgttgc ggcgtttgcc gccgtggacc aacagggtat
    16261 agaacgcttt attcagtctt acggcgcact ggcggcagtt gtctcgttct tgttgatgat
    16321 tttacaggcc attgctgcac cgctacctgc gtttttgatc acctttgcca atgcgtcgct
    16381 gtttggcgcg ttctggggcg gcttgctgtc gtggaccagt tcgatggccg gcgcggcgct
    16441 gtgctttttt atcgccagag tgatgggccg cgaagtggtg gaaaagttaa cgggtaaaac
    16501 ggtactcaat agtatggacg gcttttttac ccgctatggt aaacacacca ttctggtatg
    16561 ccgattattg ccttttgtgc cttttgatcc cgtaagctat gctgccggtt tgacttcaat
    16621 acgttttcgc tcgtttttta tcgccaccgg gcttggtcag ttaccagcga ctattgttta
    16681 ttcctgggcg ggcagcatgt taacaggcgg tactttctgg tttgtcaccg gactgtttat
    16741 tctgtttgcc ctgaccgtgg tgatttttat ggcgaagaaa atatggcttg agcgccagaa
    16801 gaggaatgcc tgatgggttt accgccgctt agcaaaattc ctttcatttt acgtccgcag
    16861 gcgtggctgc atcgtcgcca ttacggcgag gtgctaagcc cgattcgctg gtgggggcgg
    16921 atcccgttta tcttttatct ggtgtcgata tttgtcggct ggctggagcg caaacgctca
    16981 ccgctggatc cggtagtgcg atcgcttgtc agcgcgcgca ttgcgcaaat gtgcctgtgt
    17041 gagttttgcg tggatatcac cagtatgaaa gtcgccgagc gcactggcag caccgataaa
    17101 ctgctggcag tggctgactg gcggcaaagc ccgctcttta gcgatgaaga acggctggcg
    17161 ctggagtacg cagaagccgc aagcgtaacg ccgccaacgg tcgatgatgc cctgcgcgcc
    17221 cgactggtta cgcattttga cgctcaggcg ctcaccgaac tgacggcatt gatcggactg
    17281 caaaacctgt cagcccgttt taattctgcc atggacattc ccgctcaggg gctgtgccgt
    17341 attcctgaaa aacgttctta aggagagatg atgcgccatt gtgtgtggtt gctgggattg
    17401 ttatcgctgt tttctttggc aacacatgcc agtgactggc aggaaatcaa aaacgacgcc
    17461 aaagggcaaa ccgtctggtt taacgcctgg ggcggcgata ccgcaattaa ccgctatctc
    17521 gactgggtta gcggcgagat gaaaacccat tacgatataa acctgaagat tgttcatctc
    17581 gcggatgccg cagacgcggt gaagcgcatt cagaccgaag ctgctgccgg acgcaaaaca
    17641 ggcggttcgg tggatctgct ctgggtgaac ggcgaaaact tccgcacctt aaaagaggct
    17701 aatttactgc aaaccgactg ggcagagact ctgcccaact ggcgctatgt cgacacacag
    17761 cttccggtgc gggaagattt ttcagtgcct acagaagggg ctgaatcgcc ctggggcggc
    17821 gcacaactga cgtttatcgc ccgccgcgat gttacgccac agccaccaca aacgccgcaa
    17881 gccttactgg agtttgctaa agccaatccc ggcacggtta cctatccgcg cccaccggac
    17941 tttaccggca cggcgtttct tgaacagttg ctgattatgt tgacacccga tcccgccgca
    18001 ttaaaagaag cgccggacga tgcgactttc gcccgtgtca ctgcgccctt gtggcaattt
    18061 cttgatgcgc tgcatccgta tttatggcgc gaaggaaagg atttcccgcc ttcgcccgcg
    18121 cggatggatg ctctgctgaa agctaacaca ttgcgcctgt cgctgacctt taatcctgct
    18181 catgcgcagc aaaaaatcgc cagcggcgat ttacccgcca gcagttacag ctttggcttt
    18241 agcgagggga tgattggcaa cgtccatttc gtcaccattc ccgccaacgc gaatgccagt
    18301 gctgcggcga aggtagttgc caatttcctg ctctcacccg atgcacaact gcgtaaagca
    18361 gatcccgctg tctggggcga tccttctgtt ctcgatccgc aaaaactgcc tgacgggcag
    18421 cgcgaatcat tgcaatcgag aatgccgcag gatctgccgc cggtactggc tgaaccgcac
    18481 gcaggttggg taaatgcgct ggaacaagaa tggctacacc gttacggtac gcattaatct
    18541 ttttgctgtg ggcgatgatg gcggtgattt atgcaccgct gatcccggca gttctcacgc
    18601 tgatatcgcc tgctttgtcg ttgacgcact ggcaggcgtt atttgccgat ccacagttac
    18661 cgcaggcatt actggcaacg ctggtgtcga caaccattgc ggcggtcggg gcgttgttga
    18721 ttgccctgct ggtgattgta gcgctgtggc cagggccgaa atggcagcgt atgtgcgccc
    18781 gtctgccgtg gctgctcgcc attccccatg tggcttttgc caccagcgcc cttctgctct
    18841 ttgctgacgg agggctgctt tatgactatt tcccgtattt cactccgcca atggacaaat
    18901 tgggtattgg gctgggcctc accctggcag tgaaagaaag cgcatttctg ctgtggatct
    18961 tagcggcagt attgagcgaa aaacggctgt tgcagcaact cattgtgctg gattcgctgg
    19021 gctacagccg ctggcaatgc ctgaactggc tgctgttgcc ctctgtcgcg cctgcgctgg
    19081 caatggcgat gctggcgatt gtggcctggt cgctgtcggt cgtggatgtg gcgattatcc
    19141 ttgggccagg taatcccccg acgctggcag taattagctg gcagtggtta actcagggcg
    19201 acgccgatca acaagcgaaa ggcgcactcg ccagcctgct gctgatgctg ttactcgccg
    19261 cctacgtttt gctgggctat ctgctatggc gtagctggag gcgtactatt ccccgcgtag
    19321 atggcgttcg taagcctgcc acgcctttat tgcctggcat tactctggcg agttttttac
    19381 ccttgaccgg tgtgctgtgt gtggttctgt tggcgatcct cgcggatcag tcgacgatca
    19441 atagtgaagc gctcatcaat agcctgacga tgggacttac cgcgacgttt atcgctttaa
    19501 tcctgatact ggcgtggctg gaatggggat cgtcacgtcg gcatttctgg ctatggttgc
    19561 ccattttatt acccgctctg ccactggtgg cgggccagta cacgctggcg ctatggctaa
    19621 acctagatgg aagctggacg gcggtggtct gggggtatct gctgtgggta atgccgtgga
    19681 tgctgtttat cctgcaaccc gcctggcagc gcattgattc acggttaatt ttgattgcgc
    19741 aaacactggg ctggtcgcgg tccaaaatct tcttttacgt gaaatgtccg cttatgctac
    19801 gccctacgct gattgccttc gcggtgggat tttcagtcag tattgcacag tatatgccga
    19861 cgctgtggct gggcgcgggg cgttttccga cgctcaccac tgaagcggtg gcattaagca
    19921 gcggcggcag caacggcatt ctcgccgccc aggctttatg gcaactgcta ttaccgctta
    19981 ttatttttgc cctgaccgcg ttagtcgcaa aatgggtagg ttatgtcaga caaggactcc
    20041 gctaatgctc tgcgtgaaaa atgtttcgct acgtttacca gaaagccgct tgctgacaaa
    20101 cgttaacttt acggtggata aaggtgacat tgtcacgtta atggggccgt ctggctgtgg
    20161 aaaatccact ctgttttcat ggatgattgg tggactggcc ggacagtttt cttgtacagg
    20221 ggagctatgg ctcaatgagc aacggattga catgctaccc accgcacagc gtcagattgg
    20281 cattcttttc caggatgcac tgttatttga ccagttcagc gtcgggcaaa atttactgct
    20341 ggcgctaccg gcgacactta aagggaatgc ccgacgtaat gccgtgaatg atgcacttga
    20401 gcgttcaggc cttgacggag ctttccatca ggatcctgct actttgtctg gcggtcagcg
    20461 agcgcgcgtt gctctgctac gcgcccttct cgcccagcca aaagcgttac tcctggatga
    20521 gccattcagc cgtctagatg tggctctgcg cgataatttt cgccagtggg tgttcagcga
    20581 agttcgcgcc caggcgatcc ccgtcgttca ggtaacgcac gatctccagg atgttcctgc
    20641 tgatagttct gttctggata tggcgcagtg gtcagaaaat tacaacaaac tgcgataacg
    20701 caaagttttt ctcaatgcgt cagttcagaa tggcgcactc aaaactacaa tgtcgggatt
    20761 ttcgatgaaa cgtgtttctc aaatgaccgc gctggcaatg gctttagggc tggcttgcgc
    20821 ttcttcgtgg gccgctgaac tggcgaagcc tcttacactt gaccagcttc aacaacaaaa
    20881 tggcaaagcg atagatactc gccccagcgc gttttataac ggctggccac aaaccttaaa
    20941 tggcccttct ggtcatgaac ctgccgcctt aaacctctct gctaactggc tcgacaaaat
    21001 gagcaccgaa cagctcaacg agtggatcaa gcaacataac ctgaaagccg atgctccggt
    21061 ggcgctgtac ggtaatgaca aagatgtcga cgccgtcaaa aagcgactgc aaaaagcagg
    21121 ttttacgcat atctccattc tgagtgacgc gctaagcgaa ccttcccgtc tgcaaaaact
    21181 gccgcatttt gagcagctgg tttatccgca atggctgcac gacctgcaac aaggtaaaga
    21241 ggttacggcg aaacctgccg gtgactggaa agtcattgaa gcggcctggg gcgctcctaa
    21301 gctttacctt atcagccata ttcccggcgc tgactacatc gataccaacg aagtggaaag
    21361 cgaaccgctg tggaacaaag tttctgatga acaactaaaa gcgatgctgg caaaacacgg
    21421 cattcgccat gacaccacgg tcattctgta cgggcgtgac gtatacgctg cagcgcgtgt
    21481 ggcacaaatt atgctctacg ctggcgtgaa agatgtccgc ctgctggacg gcggctggca
    21541 aacctggtcc gacgcgggac tgcccgttga gcgcggaacg ccaccgaaag tgaaagcgga
    21601 accggatttc ggcgtgaaga tcccggcaca accgcaactg atgcttgata ttgaacaagc
    21661 gcgtggactg ctgcatcgcc aggatgcatc gctggtgagc attcgttcgt ggccagaatt
    21721 tatcggtacg accagcggtt acagctatat taaaccaaaa ggtgaaatag ccggagcacg
    21781 ttggggacac gctggtagcg actcgacgca tatggaagat ttccataacc cggatggcac
    21841 catgcgtagc gccgatgata ttaccgctat gtggaaagca tggaatatca agccagatca
    21901 gcaagtttca ttctactgcg gcaccggctg gcgcgcgtca gaaaccttta tgtacgcacg
    21961 cgcaatgggc tggaataacg tctccgttta tgacggtggc tggtacgaat ggagcagcga
    22021 tccaaaaaat ccggtagcaa ccggtgaacg cggcccggac agcagtaaat aacattgcaa
    22081 ttactgacgc tggagcgact tcagcgtcag atacccactc cacacccgcg taaaggtcgt
    22141 catccagcac aacgcaccaa atacccacgc aaaccacgga aaacaggccg gaaagaggca
    22201 actcagaaca aacagcaaaa tcgtctcgct gccttcggtt aagccgccca gataataaaa
    22261 cgatttatgc gcataaccgg gattatcaat ctgatgtttc gccgccagcg cagcaaaagc
    22321 gagaaaactg ctgcccgtgc cgataaacgc aaacaacagc cagcctccgg ccagcgcgtt
    22381 ttgttctggt gcggcgagaa taaaaccaaa cggcaccagc gcgtaaaaga gaaaatcgag
    22441 agaaatatcg agaaagccgc ccgcgtcggt aagtcctctg cgccgcgcca gcgcaccgtc
    22501 cagaccatca agcagcctgt tcaacaaaat gacgattagc gccgccagat accagcccag
    22561 cgccagaaac ggcagtgcca gcacgccgat ggcaaatccc actaatgtta aaccatctgg
    22621 cgtgatgccg ggtttatcca gcaccttcac acactgatgc aacaaaggtt taattcgggg
    22681 atgaagatgg cggtctagca caggcactcc ttaaatataa agcctttccg attgagcaac
    22741 agtgcggata ttatggcatt tttcgcttat ctgcccgtgt gtaatttatg aaaatgattg
    22801 aagttgttgc cgccatcatt gaacgtgatg gcaaaatttt actcgcgcaa cgccccgccc
    22861 agagcgatca ggcgggatta tgggagtttg ccggtggtaa agtcgagccg gatgaaagcc
    22921 agcggcaggc gctggtgcgt gagttaaacg aagaactggg catcgaagca actgttggtg
    22981 actatgttgc cagccatcag cgagaagttt cggggcggat tatccatctt catgcctggc
    23041 acgtacccga cttccacggg acgttacagg cacatgaaca tcaggcgctg gtctggtgct
    23101 cacctgaaga ggcgctgcaa tatccgctgg cccctgctga cattccatta ttagaggcgt
    23161 ttatggcttt acgcgccgcc agaccagcgg attagtgcta aggtttttgt catcctgttg
    23221 gcattgcagc agtattcctt cggctttaat taccgcccct tcagaataat tttgatcctg
    23281 ataaacgcag cactgagtac agggctgcgc tgactgcccg cctgaactga acacttctgg
    23341 cggtacgttt acctccacgt ccggacgata atgcgggtta gccagtgcaa ttattggaaa
    23401 tgctaatact acggcgaaca atgctcgact cacagggaac tccttaacgt tattgtctct
    23461 gctactgata acggtagccg ggtggcaaaa ctttagcgtc tgagttatcg catttggtta
    23521 tgagattact ctcgttatta atttgctttc ctgggtcatt tttttcttgc ttaccgtcac
    23581 attcttgatg gtatagtcga aaactgcaaa agcacatgac ataaacaaca taagcataat
    23641 cgtattaata tataagggtt ttatatctat ggatcagaca tattctctgg agtcattcct
    23701 caaccatgtc caaaagcgcg acccgaatca aaccgagttc gcgcaagccg ttcgtgaagt
    23761 aatgaccaca ctctggcctt ttcttgaaca aaatcccaaa tatcgtcaga tgtcattact
    23821 ggagcgtctg gttgaaccgg agcgcgtgat ccaatttcgc gtagtatggg ttgatgatcg
    23881 caaccaggtg caggtcaacc gtgcatggcg tgtgcagttc agctctgcca tcggcccgta
    23941 caaaggcggt atgcgcttcc atccgtcagt taacctctcc attctcaaat tcctcggctt
    24001 cgaacaaacc ttcaaaaatg ccctgactac tctaccgatg ggcggtggta aaggcggcag
    24061 cgatttcgac ccgaaaggga aaagcgaagg cgaagttatg cgtttttgcc aggcgctgat
    24121 gactgaactg tatcgccacc tgggcgcgga tactgacgtt ccggcaggtg atatcggcgt
    24181 tggtggtcgt gaagtcggct ttatggcggg gatgatgaaa aagctctcca acaataccgc
    24241 ctgcgtcttc accggtaagg gcctttcatt tggcggcagt cttattcgcc cggaagctac
    24301 cggctacggt ctggtttatt tcaccgaagc aatgctaaaa cgccacggta tgggttttga
    24361 agggatgcga gtttccgttt ctggctccgg caacgtcgcc cagtacgcta tcgaaaaagc
    24421 gatggaattt ggtgctcgtg tgatcactgc gtcagactcc agcggcactg tagttgatga
    24481 aagcggattc acgaaagaga aactggcacg tcttatcgaa atcaaagcca gccgcgatgg
    24541 tcgagtggct gattacgcca aagaatttgg tctggtctat ctcgaaggcc aacaaccgtg
    24601 gtctgtaccg gttgatatcg ccctgccttg cgccacccag aacgaactgg atgttgacgc
    24661 cgcgcatcag cttatcgcca atggcgttaa agccgtcgcc gaaggggcaa atatgccgac
    24721 caccatcgaa gcgactgaac tgttccagca ggcaggcgta ctgtttgcac cgggtaaagc
    24781 ggctaatgct ggcggtgtag caacatcggg cctggaaatg gcgcaaaacg ctgcgcgcct
    24841 gggctggaaa gccgagaaag ttgacgcacg tttgcatcac atcatgctgg atatccacca
    24901 tgcctgtgtt gagcatggtg gtgaaggtga gcaaaccaac tacgtgcagg gggcgaacat
    24961 tgccggtttt gtgaaggttg cggatgcgat gttaagccag ggcgtgattt aagacaattg
    25021 tgccggatac agcgcaaacg ccttatccgg ccataaaaat cttcagtatc agatggttga
    25081 aagaaccttt atattatcac tccattgccc gcctcttctg atggcgggct ttttttcggc
    25141 gctctcttgc gtggcgaatc ctttttcttc ccttctccac cgccgggagc cattattccc
    25201 cgaaactgcc ttaccgacgt gcgctttgcc tgatcaatca actggtacaa tgtccccacc
    25261 agcggctgca taaagtcctg atagcgacac tgcttttcgc tgatttgcgt cagcaccgat
    25321 tcccagtgcg cggtcatgtc cggtcgcgtc gccatttctg gcagcgaatg gaatagcgct
    25381 tttccggcgt cggtggagtg gatatagcgc ccttttttgg tcaggaagcc tcgcttgaac
    25441 agcagttcaa taatcccggc gcgagtcgct tctgtcccca gaccatcggt cgcacgaagg
    25501 atctttttca gatctttgtc ctgcacaaaa cgtgctatcc cggtcatcgc tgaaagcagt
    25561 gttgcatcgg taaaatggcg cggcggctgg gtttgccgct ctaccacttc acctttttca
    25621 cacagcaact catcgccttt cgccaccaca ggcagcggcg tgccgtcgtt ttcttcatcg
    25681 cgctctttgc tgcctaacag tgtgcgccag cctgcttcag caagaaaacg ggctttagcg
    25741 acaaatttgc ctttggcaat gtccagttcg ataacacact tgcggaacac cgcatccggg
    25801 cagaactgca tcaaatactg acgggcaatc aggttataga ccttcgcttc gttctccgtc
    25861 aggttgatcg cagaactccg tgcggtcggg atgatggcgt ggtgcgcatc gacttttttg
    25921 tcatcccaac agcggttgcg tatatctgga tcgaccactg gctgcggcaa cagatctggt
    25981 gcatgaacgc tgatagcatt catcaccgcg tggcgtccgg caaaatgttc ttctggcaaa
    26041 tagcggcaat cggaacgcgg ataagtgatt agcttgtgcg tttcgtacag cttctggcag
    26101 atatcaagca cgttctgcgc gctcagacca aaacgtttag ccgcttcaat ctgcaacgcc
    26161 gaaagcgaaa aaggcagcgg cgcggattct gattcccgtt tatcgttata gctggtgaca
    26221 atagctggtt gaccactaat gcggttaacc acatgctccg ccagtggacg atgtaataag
    26281 cgcccttctt catcctggta cggttcacac gcttcgctcg gttgccagat agcggtaaac
    26341 cgctcatcgg caggtgtcac gatatgtgct ttgacttcaa agaagtcttt cgccacgaag
    26401 ttttcaatct cttcgtcgcg gcgcaccacc agtccgagta cgggcgtctg cacgcgtccc
    26461 acggaaagca cgccctgata accggcattg cgaccgagaa tggtatacgc acgggtcata
    26521 ttaatgccgt acagccagtc agctcgcgca cgcgccagtg cagaaacgca cagcggtaca
    26581 aactcgctat tggagcgaag acgatcgatc gcccgctcaa ccgcctgcgg gttcaggtcg
    26641 tttatcaaac aacgctgtac ctgctggcgc ttttccggtg ccagttgcag gtagtccagc
    26701 acttcatcca ccagcagttg cccttcgcga tccgggtctc cggcgtgaac gatttcgctg
    26761 gcttcatgca ggaagcgttt gatgacatta agttgtttag tcacggaggg tcggggctgt
    26821 aattgccact tttccgggac aatcggcaaa tccgcaagat tccagcgcgc atagcggctg
    26881 tcgtaggcgt ctggctgcgc ctgctcaagc aggtgaccga tacaccaggt caccacctga
    26941 ccattaccgc actcgataaa gccatcgcct ttccggtgcg gtttaggcag gacatcagca
    27001 atggcgcgcg ccagactcgg tttttcggca ataaacaacc gcattgaatt aacggatctc
    27061 aaccatggca cgaccgccgc gcgctggcac cagttcgcca atcgccgtca gttcaatgcc
    27121 aaactcggcg gctgtagctt tgacctcatt ttctgcttcc ggcatgaccg ccagcagcaa
    27181 accgccagaa gtttgcggat cgcacagcag ttcgcgcact tcacgcggca tttcacccat
    27241 cagatgaccg tagctggcaa agttacgttc ggtgccgcca ggcactgcgc ccaacttaat
    27301 gtactcttca acccccggca atttcgggat cgcgtcatag tcgacgcgcg cctgcacacc
    27361 tgcgccctga cacatttcgc ttaaatgccc cagtaaacca aaacccgtaa cgtcggtcat
    27421 cgcttttacg ccttcgatgt tggcaaagga cgcgcctgcg atgttcatcc ggcacatcac
    27481 ttccgtcgcc agtccctgat gttctggttt cagcagtgat tttttctcag ccgtggtaag
    27541 aacgccaatc cccagaggtt tcgtcaggaa cagtttgcat ccggcttgtg cggtactgtt
    27601 tttcttcacc cgctcggtag gtacgatccc cgttaccgcc agaccaaaaa tcggctctgg
    27661 cgcatcgatg gagtgaccgc cagccagcgc aatccccgcc tgacggcatg catggcgtcc
    27721 accttcggtc acttcgcggg caatttccgg ggaaagtttg ttaatcggcc agccgaggat
    27781 cgcaatcgcc ataatcggtt tgccgcccat tgcgaagata tcgctgatgg cgttagtcgc
    27841 cgcaatgcgg ccaaaatcga aaggattatc aacgatcggc ataaagaagt cggtggtact
    27901 gataacgctg gtgccattgc ccagatcgta taccgccgca tcgtcgcggg tttcattacc
    27961 cacaagcaaa ttcggatcaa caaacttcgc ctgctcactg tgcaggatgg tttccaacac
    28021 ttttggggaa attttacagc cgcaaccagc gccgtggctg tattgggtca aacgaatcga
    28081 gttctcgctc atggacatct cctgtcaatg caatccgggt atggtaaccc tcattccatg
    28141 aagtgataag tgagagtgtc tgaattcctg cgcctttgct cacaatccag acagtttcgc
    28201 gacaattatc agaaataagt cacaaacggc gtcgggtccg ggacgtttat cgacgtagat
    28261 gctttcaact gcggcgtacc gaggtagaga aaaccgacaa ttttatcctg ctcacggcaa
    28321 ccgaatgctt cacgcactac cggactttca gttaatgcac cactgcgcca gatgccgcca
    28381 aacccctggg caactgctgc catttgcatc gccatgaccg cgcatcccgc agacatttcc
    28441 tgttcccagc gcgggacttt atgattttct tcgcatttcg ccaccaccgt tatgatgagc
    28501 ggtgcgcgga acggcgcatt acgcgctttg tcgatggctt tgttatcact accggcagca
    28561 atcgccccct gctccagtac ggcgctgaaa cgctcgcgcc cttccccttc aatcacaaag
    28621 aaatgccacg gttgcatgga cttatggtcc ggcgcacgca tagccgcacg caggatgttt
    28681 tgcagttgtt cacccgttgg cgcaggttca gccaggcggg aggcgctacg gcgattgatc
    28741 aatagttcga gtgcatccat ttgattaact cctgtcatga tatttattca caaaattaac
    28801 acgagagtgg attttgttac agcccagtcc gcaattcctg ctgacaagtg ccggttgggt
    28861 cattacgata accacatcta ttgcgcctga aacaggtgtg accttaagtt gggagaatac
    28921 atgcgaaccc tttggcgatt tattgccgga ttttttaaat ggacgtggcg tctgctgaat
    28981 ttcgtccgtg aaatggtact taacctgttc tttatttttc tcgtgctggt tggtgtgggg
    29041 atctggatgc aggtcagtgg tggtgattcg aaagaaacgg ccagtcgtgg cgcactgctg
    29101 ctggacattt ctggtgtgat cgtcgataaa cccgacagtt ctcagcggtt tagtaaatta
    29161 agccgccagc tgcttggtgc cagttccgat cgtctgcagg aaaactcact gtttgatatc
    29221 gtcaacacca ttcgccaggc gaaggacgac cgcaatatca ccggtattgt gatggatctg
    29281 aaaaacttcg caggcggcga ccagccgtct atgcagtaca tcggcaaagc tctgaaagag
    29341 tttcgtgata gcgggaaacc ggtttatgcc gttggcgaga actacagcca ggggcaatat
    29401 tatctcgcca gtttcgccaa taaaatttgg ctgtctccgc aaggcgtggt tgatctgcac
    29461 ggctttgcca ccaacggtct gtactacaaa tcattactgg ataagctgaa agtttccacc
    29521 catgtgttcc gcgtgggtac gtataaatct gccgttgaac cgtttattcg tgatgatatg
    29581 tcgccggcag cccgcgaagc tgacagccgc tggattggtg agctgtggca aaactatctg
    29641 aatactgttg ccgctaaccg gcagatccct gctcagcagg tattccctgg cgcgcaaggg
    29701 ttgcttgagg gtttaaccaa aaccggtggc gataccgcga aatatgcgct ggaaaacaag
    29761 ctggtcgatg cactggcatc gagtgccgaa atcgaaaaaa ctctgaccaa agagttcggc
    29821 tggagcaaga ctgataaaaa ttatcgcgcc atcagttatt acgattacgc attgaaaacg
    29881 ccggcagata ccggtgacag catcggagtc gtcttcgcta atggcgcaat tatggatggc
    29941 gaggaaactc aggggaatgt tggcggtgat accactgcgg cacaaatccg cgacgctcgc
    30001 cttgacccga aagtgaaagc gattgtcctg cgtgttaata gcccaggcgg cagcgttacc
    30061 gcgtctgaag tgattcgcgc tgaactggca gcagcccggg cagcgggtaa gcctgtggtt
    30121 gtatcgatgg gcggcatggc ggcatctggt ggttactgga tttccacacc agctaattac
    30181 attgtggcta accccagcac cctgaccggt tctatcggta tcttcggcgt gatcaccacc
    30241 gtagaaaata gtctggattc gattggtgtt catactgatg gtgtctcaac ttcaccgctg
    30301 gcggatgttt ctatcaccag ggcactgccg ccggaagcgc agcagatgat gcagttaagc
    30361 attgagaatg gctataaacg ctttatcacg ctggttgctg atgcgcgtca ttcaacgcca
    30421 gagcagattg ataaaatcgc ccagggccat gtctggaccg gtcaggatgc aaaagctaac
    30481 gggctggtcg atagtctcgg ggatttcgat gatgcggtcg ccaaagcagc agagctggca
    30541 aaagtgaaac agtggcatct ggaatactac gttgatgaac cgaccttctt cgacaaagtg
    30601 atggacaaca tgtctggttc tgtccgggca atgttgccag atgcgttcca ggccatgtta
    30661 cctgcaccgc tggcctcggt agcctctacc gttaaaagtg aaagcgacaa gctggcagcg
    30721 tttaacgacc cacaaaaccg ttatgcgttt tgcctgacct gcgccaacgt gcgttaagtc
    30781 ttgtactgag tggccgacag atcgtcggcc acattttttt acctcgacga atcctcttcc
    30841 cgctgtctcg ccccatattt ccttatccgc atagtatcag gtgcgctccc ccttcctcac
    30901 gtatatactt ttgctctttc gatatcattc atatcaatat catgcaaaag aaatcaattt
    30961 acgtcgccta cacgggcggg accatcggga tgcagcgttc cgagcagggt tatattccgg
    31021 tgtcaggtca tctacagcgc caactggcgc tgatgccgga attccatcgc ccggagatgc
    31081 cagatttcac tattcatgaa tatacgccat tgatggattc ttctgatatg acgccagaag
    31141 actggcagca tattgctgaa gatattaaag cgcactatga cgactatgat ggttttgtca
    31201 ttctgcacgg cacagacacg atggcgtata ccgcctctgc actgtcgttc atgctcgaga
    31261 atctcggtaa accggtcatt gtgacagggt cacaaatccc gctggctgag ttacgctctg
    31321 acggacaaat taatctgctg aatgcgttgt acgttgcggc gaattatcct atcaacgaag
    31381 taactctctt ttttaataat cgactgtatc gtggcaacag aaccaccaaa gcccatgccg
    31441 atggttttga tgcgtttgcc tctccaaacc ttcctccgtt actggaagca ggtatccata
    31501 ttcgccgttt gaatacgcca cccgccccgc acggtgaagg ggaattgatc gttcatccaa
    31561 tcacgccgca accaattggc gtagtgacga tttatccggg gatttctgct gacgtcgtgc
    31621 gcaattttct gcgccaacct gtgaaagcat tgattctgcg ctcatatggc gtgggtaatg
    31681 cgccacaaaa caaagccttc ctgcaggaat tacaagaagc cagcgatcgc ggtattgtgg
    31741 tggtcaacct gacacaatgt atgtccggta aagtgaacat gggtggttat gccaccggta
    31801 acgccctcgc ccatgccggc gttattggcg gtgcagatat gactgtagaa gccacgctaa
    31861 ccaaactgca ttacctgctt agccaggaac ttgatactga aaccattcgc aaggccatga
    31921 gccaaaacct gcgtggcgaa ctgacgccgg atgattaagg agactgcaat gcaccctcgc
    31981 gccctgttac tggtcgattt acaaaatgat ttctgtgctg gtggcgcgct cgccgtgccg
    32041 gaaggtgaca gtacggtgga tgtcgctaac cgcctgattg actggtgcca gtcgcgcggt
    32101 gaagcggtta tcgccagtca ggactggcac ccggcgaatc acggcagttt tgccagtcag
    32161 cacggtgtag agccttatac gccaggccaa ctcgacggtt tgccacaaac cttctggcca
    32221 gatcactgtg tgcagaacag tgaaggcgca caattacatc cgttactgaa ccaaaaagcg
    32281 atcgcagcgg tgttccataa aggcgaaaat cctttagttg acagttacag tgcctttttt
    32341 gataacggcc gtcggcagaa aaccacgctc gatgactggt tacgcgatca tgaaatcgat
    32401 gaattgatca ttatgggtct ggctactgac tattgcgtga agtttaccgt gctggacgcg
    32461 ttacagttag gttataaggt taatgtgatt accgatggtt gtcgtggcgt gaatatccag
    32521 ccacaggaca gtgcgcacgc gtttatggag atgtcagcag ctggggcaac gctatatacg
    32581 ctggcagatt gggaagagac gcaggagtaa tattagttgg attcatctct ggcctacatt
    32641 tcctcaatga cattgtaggc caggtaatac ttgttagtgt tgcatttaac ctactcgcag
    32701 ctaaaacacg acatccgaac aagtctattt gccctccctt aataacaggc ttaaatacta
    32761 gacaactcat tcacttaaac atggcaatag gctttgctga gataccaaaa tcttctttta
    32821 actgttgttt gctcttcatg accatctgac catttgtgtc aattgtcata tgctgtgctt
    32881 cggtattatt gcgggcttgc cacagcatca ccagttgcag gcagttctct ttttgttcct
    32941 cggtcaacgt aacgccatca ggccatttcc ccagttcaac ggcggtcgac aaacgctggt
    33001 atacctcagg cgtcatgctg ttgataatgt catcaagatt cataattttt ccgctcctgt
    33061 ggaataagtt gctgaatcgt tttttcaacc gttgatttct tcgccgtttt cgccatcagt
    33121 gaagcgtaaa gaagcagagt taacacaata acgttcgccc gttggctgcg gcccgtcagg
    33181 gaagacatgg cccagatggg catcgcagtt accgcaacga atttctatgc gctgcattcc
    33241 atgtgacaag tctttgatat aacgaataga ttcttcactc accggttcgt agaaactggg
    33301 ccagccacag ccggaatcat acttggtttg ggaatgaaac agcggggcat cgcagatcaa
    33361 acagtgatat acgccgtcac gcttgttatg cagtaaacga cccgtaaatg gcggttctgt
    33421 cccatgattc tgcgtcacgt agaactgcat ctcggacaaa ttttttttca gttcttctgc
    33481 cgaaggttta ttagccattt gctcacatct cactttaatc gtgctcacat tacgtgactg
    33541 attctaacaa aacattaaca ccaactggca aaattttgtc ctaaacttga tctcgacgaa
    33601 atggctgcac ctaaatcgtg atgaaaatca catttttatc gtaattgccc tttaaaattc
    33661 ggggcgccga ccccatgtgg tctcaagccc aaaggaagag tgaggcgagt cagtcgcgta
    33721 atgcttaggc acaggattga tttgtcgcaa tgattgacac gattccgctt gacgctgcgt
    33781 aaggtttttg taattttaca ggcaaccttt tattcactaa caaatagctg gtggaatata
    33841 tgactatcaa agtaggtatc aacggttttg gccgtatcgg tcgcattgtt ttccgtgctg
    33901 ctcagaaacg ttctgacatc gagatcgttg caatcaacga cctgttagac gctgattaca
    33961 tggcatacat gctgaaatat gactccactc acggccgttt cgacggtacc gttgaagtga
    34021 aagacggtca tctgatcgtt aacggtaaaa aaatccgtgt taccgctgaa cgtgatccgg
    34081 ctaacctgaa atgggacgaa gttggtgttg acgttgtcgc tgaagcaact ggtctgttcc
    34141 tgactgacga aactgctcgt aaacacatca ccgctggcgc gaagaaagtg gttatgactg
    34201 gtccgtctaa agacaacact ccgatgttcg ttaaaggcgc taacttcgac aaatatgctg
    34261 gccaggacat cgtttccaac gcttcctgca ccaccaactg cctggctccg ctggctaaag
    34321 ttatcaacga taacttcggc atcatcgaag gtctgatgac caccgttcac gctactaccg
    34381 ctactcagaa aaccgttgat ggcccgtctc acaaagactg gcgcggcggc cgcggtgctt
    34441 cccagaacat catcccgtcc tctaccggtg ctgctaaagc tgtaggtaaa gtactgccag
    34501 aactgaatgg caaactgact ggtatggcgt tccgcgttcc gaccccgaac gtatccgtag
    34561 ttgacctgac cgttcgtctg gaaaaagctg caacttacga gcagatcaaa gctgccgtta
    34621 aagctgctgc tgaaggcgaa atgaaaggcg ttctgggcta caccgaagat gacgtagtat
    34681 ctaccgattt caacggcgaa gtttgcactt ccgtgttcga tgctaaagct ggtatcgctc
    34741 tgaacgacaa cttcgtgaaa ctggtatcct ggtacgacaa cgaaaccggt tactccaaca
    34801 aagttctgga cctgatcgct cacatctcca aataagttga gatgacactg tgatctaaaa
    34861 agagcgactt cggtcgct
//
LOCUS       DAZFLD010000018        34170 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.018, whole genome shotgun sequence.
ACCESSION   DAZFLD010000018 DAZFLD010000000
VERSION     DAZFLD010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 34170)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34170)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34170
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.018"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(27..1886)
                     /gene="gss"
                     /locus_tag="ACHKBL_000821"
     CDS             complement(27..1886)
                     /gene="gss"
                     /locus_tag="ACHKBL_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutathionylspermidine
                     amidase/synthase"
                     /protein_id="HFO5817734.1"
                     /translation="MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVF
                     RSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQ
                     AFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHSPLPQGQQ
                     WTRELEMVVENGCYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLEN
                     KGQFDGKWLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELHLMYLH
                     ATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCMDERGLKVYEYNAD
                     SASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARPFVHIMQDKDIEE
                     NYHAQFMEQALHQAGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQ
                     IREVSDREFAAVPIRTGHPQNEVRLIDVLLRPEVLVFEPLWTVIPGNKAILPILWSLF
                     PHHRYLLDTDFSVNDELVKTGYAVKPIAGRCGSNIDLVSHHEEVLDKTSGKFAEQKNI
                     YQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVKE"
     gene            2091..2957
                     /gene="yghU"
                     /locus_tag="ACHKBL_000822"
     CDS             2091..2957
                     /gene="yghU"
                     /locus_tag="ACHKBL_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HFO5817735.1"
                     /translation="MTDNTYQPAKVWTWDKSAGGAFANINRPVSGPTHEKTLPVGKHP
                     LQLYSLGTPNGQKVTIMLEELLALGVTGAEYDAWLIRIGDGDQFSSGFVEVNPNSKIP
                     ALRDHTHNPPIRVFESGSILLYLAEKFGYFLPQDLAKRTETMNWLFWLQGAAPFLGGG
                     FGHFYHYAPVKIEYAINRFTMEAKRLLDVLDKQLAQHKFVAGDEYTIADMAIWPWFGN
                     VVLGGVYDAAEFLDAGSYKHVQRWAKEVGERPAVKRGRIVNRINGPLNEQLHERHDAS
                     DFETNTEDKRQG"
     gene            complement(3080..3328)
                     /gene="hybG"
                     /locus_tag="ACHKBL_000823"
     CDS             complement(3080..3328)
                     /gene="hybG"
                     /locus_tag="ACHKBL_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation factor HybG"
                     /protein_id="HFO5817736.1"
                     /translation="MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADL
                     LGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYDITSA"
     gene            complement(3341..3682)
                     /gene="hypA"
                     /locus_tag="ACHKBL_000824"
     CDS             complement(3341..3682)
                     /gene="hypA"
                     /locus_tag="ACHKBL_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HFO5817737.1"
                     /translation="MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAV
                     RFSFEIVCHGTVAQGCDLHIVYKPAQAWCWDCSQVVEIHQHDAQCPICHGERLRVDTG
                     DSLIVKSIEVE"
     gene            complement(3675..4163)
                     /gene="hybE"
                     /locus_tag="ACHKBL_000825"
     CDS             complement(3675..4163)
                     /gene="hybE"
                     /locus_tag="ACHKBL_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-2 assembly chaperone"
                     /protein_id="HFO5817738.1"
                     /translation="MTEEIAGFQTSPKAQVQAAFEEIARRSMHDLSFLHPSMPVYVSD
                     FTLFEGQWTGCVITPWMLSAVIFPGPDQLWPLRKVSEKIGLQLPYGTMTFTVGELDGV
                     SQYLSCSLMSPLSHSMSIEEGQRLTDDCARMLLSLPVTNPDVPHAGRRALLFGRRSGE
                     NA"
     gene            complement(4156..4650)
                     /gene="hybD"
                     /locus_tag="ACHKBL_000826"
     CDS             complement(4156..4650)
                     /gene="hybD"
                     /locus_tag="ACHKBL_000826"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HFO5817739.1"
                     /translation="MRILVLGVGNILLTDEAIGVRIVEALEQRYILPDYVEILDGGTA
                     GMELLGDMANRDHLIIADAIVSKKNAPGTMMILRDEEVPALFTNKISPHQLGLADVLS
                     ALRFTGEFPKKLTLVGVIPESLEPHIGLTPTVEAMIEPALEQVLAALRESGVEAIPRE
                     AIHD"
     gene            complement(4650..6353)
                     /gene="hybC"
                     /locus_tag="ACHKBL_000827"
     CDS             complement(4650..6353)
                     /gene="hybC"
                     /locus_tag="ACHKBL_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 large subunit"
                     /protein_id="HFO5817740.1"
                     /translation="MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEI
                     VKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTH
                     DHIVHFYQLSALDWVDITSALQADPTKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
                     SGQLGIFANGYWGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNL
                     AVGGVANPINLDGLGVLNLERLMYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRG
                     KGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDEYLIKGIQESAKHS
                     WYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRE
                     STQNKLNEIVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNI
                     GKGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVPSTWNSGP
                     RNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNEVVSVKV
                     L"
     gene            complement(6350..7528)
                     /gene="hybB"
                     /locus_tag="ACHKBL_000828"
     CDS             complement(6350..7528)
                     /gene="hybB"
                     /locus_tag="ACHKBL_000828"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HFO5817741.1"
                     /translation="MSHDPQPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSD
                     LNGGFPWGVWIAFDLLIGTGFACGGWALAWAVYVFNRGQYHPLVRPALLASLFGYSLG
                     GLSITIDVGRYWNLPYFYIPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWK
                     VSLQRLNKVMFFIIALGALLPTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAF
                     IMGFSIVIFEGSLVQAGLRGNGPDEKSLFVKLTNTISVLLAIFIVLRFGELIYRDKLS
                     LAFAGDFYSAMFWIEVLLMLFPLVVLRVAKLRNDSRMLFLSALSALLGCATWRLTYSL
                     VAFNPGGGYAYFPTWEELLISIGFVAIEICTYIVLIRLLPILPPLKQNDHNRHEASKA
                     "
     gene            complement(7518..8504)
                     /gene="hybA"
                     /locus_tag="ACHKBL_000829"
     CDS             complement(7518..8504)
                     /gene="hybA"
                     /locus_tag="ACHKBL_000829"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HFO5817742.1"
                     /translation="MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDS
                     TLCVGCQACVTKCQDINFPERNPQGEQTWSNNDKLSPYTNNIIQVWTSGSGVNKDQEE
                     NGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
                     KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRL
                     ALKPGSEYHYPRQTLKSGDTYLHTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPK
                     LDDLSTGARSENIQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHHDGGDDHES"
     gene            complement(8507..9625)
                     /gene="hybO"
                     /locus_tag="ACHKBL_000830"
     CDS             complement(8507..9625)
                     /gene="hybO"
                     /locus_tag="ACHKBL_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 small subunit"
                     /protein_id="HFO5817743.1"
                     /translation="MTGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMAESVT
                     NPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLETISLEYHEVLSAAFGHQVEENK
                     HNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGAAAIIAIGSCSAW
                     GGVAAAGVNPTGAVSLQEVLPGKTVINIPGCPPNPHNFLATVAHIITYGKPPKLDDKN
                     RPTFAYGRLIHEHCERRPHFDAGRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQ
                     FCDVGGVWPVAIGHPCYGCNEEGIGFHKGIHQLANVENQTPRSQKPDVNAKEGGNVSA
                     GAIGLLGGVVGLVAGVSVMAVRELGRQQKKDNADSRGE"
     gene            complement(9814..10101)
                     /gene="yghW"
                     /locus_tag="ACHKBL_000831"
     CDS             complement(9814..10101)
                     /gene="yghW"
                     /locus_tag="ACHKBL_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2623 family protein YghW"
                     /protein_id="HFO5817744.1"
                     /translation="MNNHFGKGLMAGLKATHADSAVNVTKYCADYKRGFVLGYSHRMY
                     EKTGDRQLSAWEAGILTRRYGLDKEMVMDFFRENNSCSTLRFFMAGYRLEN"
     gene            complement(10220..11107)
                     /gene="yghX"
                     /locus_tag="ACHKBL_000832"
     CDS             complement(10220..11107)
                     /gene="yghX"
                     /locus_tag="ACHKBL_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YghX family hydrolase"
                     /protein_id="HFO5817745.1"
                     /translation="MPRLTAKDFPQELLDYYDYYAHGKISKREFLNLAAKYAVGGMTA
                     LALFDLLKPNYALATQVEFTDPEIVAEYITYPSPNGHGEVRGYLVKPAKMSGKTPAVV
                     VVHENRGLNPYIEDVTRRVAKAGYIALAPDGLSSVGGYPGNDDKGRELQQQVDPTKLM
                     NDFFAAIEFMQSYPQATGKVGITGFCYGGGVSNAAAVAYPELACAVPFYGRQAPTADV
                     AKIEAPLLLHYAELDTRINEGWPAYEAALKANNKVYEAYIYPGVNHGFHNDSTPRYDK
                     SAADLAWQRTLKWFDKYLS"
     gene            11264..12304
                     /gene="gpr"
                     /locus_tag="ACHKBL_000833"
     CDS             11264..12304
                     /gene="gpr"
                     /locus_tag="ACHKBL_000833"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HFO5817746.1"
                     /translation="MVWLANPERYGQMQYRYCGKSGLRLPALSLGLWHNFGHVNALES
                     QRAILRKAFDLGITHFDLANNYGPPPGSAEENFGRLLREDFAAYRDELIISTKAGYDM
                     WPGPYGSGGSRKYLLASLDQSLKRMGLEYVDIFYSHRVDENTPMEETASALAHAVQSG
                     KALYVGISSYSPERTQKMVELLHEWKIPLLIHQPSYNLLNRWVDKSGLLDTLQNNGVG
                     CIAFTPLAQGLLTGKYLNAIPQDSRMHREGNKVRGLTPKMLTEANLNSLRLLHEMAQQ
                     RGQSMAQMALSWLLKDERVTSVLIGASRAEQLEENVQALNNLTFSTEELAQIDQHIAD
                     GELNLWQASSDK"
     gene            complement(12344..12838)
                     /gene="yqhA"
                     /locus_tag="ACHKBL_000834"
     CDS             complement(12344..12838)
                     /gene="yqhA"
                     /locus_tag="ACHKBL_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HFO5817747.1"
                     /translation="MERFLENAMYASRWLLAPVYFGLSLALVALALKFFQEIIHVLPN
                     IFSMAESDLILVLLSLVDMTLVGGLLVMVMFSGYENFVSQLDISENKEKLNWLGKMDA
                     TSLKNKVAASIVAISSIHLLRVFMDAKNVPDNKLMWYVIIHLTFVLSAFVMGYLDRLT
                     RHNH"
     gene            13029..13913
                     /gene="yghA"
                     /locus_tag="ACHKBL_000835"
     CDS             13029..13913
                     /gene="yghA"
                     /locus_tag="ACHKBL_000835"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HFO5817748.1"
                     /translation="MSHLKDPTTQYYTGEYPKQKQPTPGIQAKMTPVPDCGEKTYVGS
                     GRLKDRKALVTGGDSGIGRAAAIAYAREGADVAISYLPVEEEDAQDVKKIIEECGRKA
                     VLLPGDLSDEKFARSLVHEAHKALGGLDIMALVAGKQVAITDIADLTSEQFQKTFAIN
                     VFALFWLTQEAIPLLPKGASIITTSSIQAYQPSPHLLDYAATKAAILNYSRGLAKQVA
                     EKGIRVNIVAPGPIWTALQISGGQTQDKIPQFGQKTPMKRAGQPAELAPVYVYLASQE
                     ASYVTAEVHGVCGGEHLG"
     gene            complement(13952..14377)
                     /gene="exbD"
                     /locus_tag="ACHKBL_000836"
     CDS             complement(13952..14377)
                     /gene="exbD"
                     /locus_tag="ACHKBL_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HFO5817749.1"
                     /translation="MAMHLNENLDDNGEMHDINVTPFIDVMLVLLIIFMVAAPLATVD
                     VKVNLPASTSTPQPRPEKPVYLSVKADNSMFIGNDPVTDETMITALNALTEGKKDTTV
                     FFRADKTVDYETLMKVMDTLHQAGYLKIGLVGEETAKAK"
     gene            complement(14384..15118)
                     /gene="exbB"
                     /locus_tag="ACHKBL_000837"
     CDS             complement(14384..15118)
                     /gene="exbB"
                     /locus_tag="ACHKBL_000837"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HFO5817750.1"
                     /translation="MGNNLMQTDLSVWGMYQHADIVVKCVMIGLILASVVTWAIFFSK
                     SVEFFNQKRRLKREQQLLAEARSLNQANDIAADFGSKSLSLHLLNEAQNELELSEGSD
                     DNEGIKERTSFRLERRVAAVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIA
                     QTQTTNLAVVAPGIAEALLATAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLL
                     LQSRDLDLEASAAAHPVRVAQKLRAG"
     gene            15370..16557
                     /gene="metC"
                     /locus_tag="ACHKBL_000838"
     CDS             15370..16557
                     /gene="metC"
                     /locus_tag="ACHKBL_000838"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HFO5817751.1"
                     /translation="MADKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFDSVEAKKH
                     ATRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCVLFPCGAAAVANSILAFVEQGD
                     HVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLLPNTKIVFLESPGSIT
                     MEVHDVPAIVAAVRSVVPDAIIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDA
                     MIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEW
                     LAEHPQVARVNHPALPGSKGHEFWKRDFTGSSGLFSFVLKKKLNDEELANYLDNFSLF
                     SMAYSWGGYESLILANQPEHIAAIRPQGEIDFSGTLIRLHIGLEDVDDLIADLDAGFA
                     RIV"
     gene            16697..17356
                     /gene="yghB"
                     /locus_tag="ACHKBL_000839"
     CDS             16697..17356
                     /gene="yghB"
                     /locus_tag="ACHKBL_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family general envelope maintenance protein
                     YghB"
                     /protein_id="HFO5817752.1"
                     /translation="MAVIQDIIAALWQHDFAALADPHIVSVVYFVMFATLFLENGLLP
                     ASFLPGDSLLILAGALIAQGVMDFLPTIAILTAAASLGCWLSYIQGRWLGNTKTVKGW
                     LAQLPAKYHQRATCMFDRHGLLALLAGRFLAFVRTLLPTMAGISGLPNRRFQFFNWLS
                     GLLWVSVVTSFGYALSMIPFVKRHEDQVMTFLMILPIALLTAGLLGTLFVVIKKKYCN
                     A"
     gene            complement(17396..18295)
                     /gene="yqhC"
                     /locus_tag="ACHKBL_000840"
     CDS             complement(17396..18295)
                     /gene="yqhC"
                     /locus_tag="ACHKBL_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YqhC"
                     /protein_id="HFO5817753.1"
                     /translation="MKREEICRLLADKVNKLKNKENSLSELLPDVRLLYGETPFARTP
                     VMYEPGIIILFSGHKIGYINERVFRYDANEYLLLTVPLPFECETYATSEVPLAGLRLN
                     VDILQLQELLMDIGEDEHFQPSMAASGINSATLSEEILCAAERLLDVMERPLDARILG
                     KQIIREILYYVLTGPCGGALLALVSRQTHFSLISRVLKRIENKYTENLSVEQLAAEAN
                     MSVSAFHHNFKSVTSTSPLQYLKNYRLHKARMMIIHDGMKASAAAMRVGYESASQFSR
                     EFKRYFGVTPGEDAARMRAMQGN"
     gene            18489..19652
                     /gene="yqhD"
                     /locus_tag="ACHKBL_000841"
     CDS             18489..19652
                     /gene="yqhD"
                     /locus_tag="ACHKBL_000841"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:1990362 - butanol dehydrogenase (NAD+)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HFO5817754.1"
                     /translation="MNNFNLHTPTRILFGKGAIAGLREQIPHDARVLITYGGGSVKKT
                     GVLDQVLDALKGMDVLEFGGIEPNPAYETLMNAVKLVREQKVTFLLAVGGGSVLDGTK
                     FIAAAANYPENIDPWHILQTGGKEIKSAIPMGCVLTLPATGSESNAGAVISRKTTGDK
                     QAFHSAHVQPVFAVLDPVYTYTLPPRQVANGVVDAFVHTVEQYVTKPVDAKIQDRFAE
                     GILLTLIEDGPKALKEPENYDVRANVMWAATQALNGLIGAGVPQDWATHMLGHELTAM
                     HGLDHAQTLAIVLPALWNEKRDTKRAKLLQYAERVWNITEGSDDERIDAAIAATRNFF
                     EQLGVPTHLSDYGLDGSSIPALLKKLEEHGMTQLGENHDITLDVSRRIYEAAR"
     gene            19757..20584
                     /gene="dkgA"
                     /locus_tag="ACHKBL_000842"
     CDS             19757..20584
                     /gene="dkgA"
                     /locus_tag="ACHKBL_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050580 - 2,5-didehydrogluconate reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgA"
                     /protein_id="HFO5817755.1"
                     /translation="MANPTVIKLQDGNVMPQLGLGVWQASNEEVITAIQKALEVGYRS
                     IDTAAAYKNEEGVGKALKNASVNREELFITTKLWNDDHKRPREALLDSLKKLQLDYID
                     LYLMHWPVPAIDHYVEAWKGMIELQKEGLIKSIGVCNFQIHHLQRLIDETGVTPVINQ
                     IELHPLMQQRQLHAWNATHKIQTESWSPLAQGGKGVFDQKVIRDLADKYGKTPAQIVI
                     RWHLDSGLVVIPKSVTPSRIVENFDVWDFRLDKDELGEIAKLDQGKRLGPDPDQFGG"
     gene            20784..21710
                     /gene="yqhG"
                     /locus_tag="ACHKBL_000843"
     CDS             20784..21710
                     /gene="yqhG"
                     /locus_tag="ACHKBL_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjP/YqhG family protein"
                     /protein_id="HFO5817756.1"
                     /translation="MKIILLFLAALASFTVHAQPPSQTVEQTVRQIYQNYKSDASTPY
                     FGETGERAITSARIQQALTLNDNLTLPGNIGWLDYDPVCDCQDFGDLVLESVAITQPD
                     ADHADAVVRFRIFKDDKEKTTQTLKMVAENGRWVIDDIVSNHGSVLQAVNSENEKTLA
                     AIASLQKEQPEAFVAELFEHIADYSWPWTWVVSDSYRQAVNAFYKTTFKTTNNPDEDM
                     RIERQFIYDNPICFGEESLFSRVDEIRVLEKTADSARIHVRFTLTNGNNEEQELVLQR
                     REGKWEIADFIRPNSGSLLKQIEAKTAARLKQ"
     gene            21761..22018
                     /gene="yqhH"
                     /locus_tag="ACHKBL_000844"
     CDS             21761..22018
                     /gene="yqhH"
                     /locus_tag="ACHKBL_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YqhH"
                     /protein_id="HFO5817757.1"
                     /translation="MKTIFTVGAVVLATCLLSGCVNEQKVNQLASNVQTLNAKIARLE
                     QDMKALRPQIYAAKSDANRANTRLDAQDYFDCLRCLRMYAE"
     gene            complement(22060..24279)
                     /gene="ygiQ"
                     /locus_tag="ACHKBL_000845"
     CDS             complement(22060..24279)
                     /gene="ygiQ"
                     /locus_tag="ACHKBL_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HFO5817758.1"
                     /translation="MSSISLIQPDRDLFSWPQYWAACFGPAPFLPMSREEMDQLGWDS
                     CDIILVTGDAYVDHPSFGMAICGRMLEAQGFRVGIIAQPDWSSKDDFMRLGKPNLFFG
                     VTAGNMDSMINRYTADRRLRHDDAYTPDNVAGKRPDRATLVYTQRCKEAWKDVPVILG
                     GIEASLRRTAHYDYWSDTVRRSVLVDSKADMLMFGNGERPLVEVAHRLAMGEPISEIR
                     DVRNTAIIVKEALPGWSGVDSTRLDTPGKIDPIPHPYGEDLPCADNKPVAPKKQEAKA
                     VTVQPPRPKPWEKTYVLLPSFEKVKGDKVLYAHASRILHHETNPGCARALMQKHGDRY
                     VWINPPAIPLSTEEMDSVFALPYKRVPHPAYGNARIPAYEMIRFSVNIMRGCFGGCSF
                     CSITEHEGRIIQSRSEDSIINEIEAIRDTVPGFTGVISDLGGPTANMYMLRCKSPRAE
                     QTCRRLSCVYPDICPHMDTNHEPTINLYRRARDLKGIKKILIASGVRYDIAVEDPRYI
                     KELATHHVGGYLKIAPEHTEEGPLSKMMKPGMGSYDRFKELFDTYSKQAGKEQYLIPY
                     FISAHPGTRDEDMVNLALWLKKHRFRLDQVQNFYPSPLANSTTMYYTGKNPLAKIGYK
                     SEDVFVPKGDKQRRLHKALLRYHDPANWPLIRQALEAMGKKHLIGSRRDCLVPAPTIE
                     EMREARRQNRNTRPALTKHTPMATQRQTPATAKKASSTQSRLQNAGAKKRPKAAVGR"
     gene            complement(24390..25802)
                     /gene="ftsP"
                     /locus_tag="ACHKBL_000846"
     CDS             complement(24390..25802)
                     /gene="ftsP"
                     /locus_tag="ACHKBL_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsP"
                     /protein_id="HFO5817759.1"
                     /translation="MSLSRRQFIQASGIALCAGAVPLKASAAGQQQPLPVPTLLESRR
                     GQPLFMTVQRAHWSFTPGTRASVWGINGRYLGPTIRVWKGDDVKLIYSNRLTENVSMT
                     VAGLQVPGPLMGGPARMMSPNADWAPVLPIRQNAATLWYHANTPNRTAQQVYNGLAGM
                     WLVEDEVSKSLPIPNHYGVDDFPVIIQDKRLDNFGTPEYNEPGSGGFVGDTLLVNGVQ
                     SPYVEVSRGWVRLRLLNASNSRRYQLQMSDGRPLHVISGDQGFLPAPVSVKQLSLAPG
                     ERREILVDMSNGDEVSITCGEAASIVDRIRGFFEPSSILVSTLVLTLRPTGLLPLVTD
                     SLPMRLLPTEIMAGSPIRSRDISLGDDPGINGQLWDVNRIDVTAQQGTWERWTVRADE
                     PQAFHIEGVMFQIRNVNGAMPFPEDRGWKDTVWVDGQVELLVYFGQPSWAHFPFYFNS
                     QTLEMADRGSIGQLLVNPVP"
     gene            complement(25877..26614)
                     /gene="plsC"
                     /locus_tag="ACHKBL_000847"
                     /gene_synonym="parF"
     CDS             complement(25877..26614)
                     /gene="plsC"
                     /locus_tag="ACHKBL_000847"
                     /gene_synonym="parF"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HFO5817760.1"
                     /translation="MLYIFRLIITVIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGR
                     LAPLFGLKVECRKPADAESYGNAIYIANHQNNYDMVTASNIVQPPTVTVGKKSLLWIP
                     FFGQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFK
                     TGAFHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELA
                     AHCRSIMEQKIAELDKEVAEREAAGKV"
     gene            complement(26848..29106)
                     /gene="parC"
                     /locus_tag="ACHKBL_000848"
     CDS             complement(26848..29106)
                     /gene="parC"
                     /locus_tag="ACHKBL_000848"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HFO5817761.1"
                     /translation="MSDMAERLALHEFTENAYLNYSMYVIMDRALPFIGDGLKPVQRR
                     IVYAMSELGLNASAKFKKSARTVGDVLGKYHPHGDSACYEAMVLMAQPFSYRYPLVDG
                     QGNWGAPDDPKSFAAMRYTESRLSKYSELLLSELGQGTADWVPNFDGTLQEPKMLPAR
                     LPNILLNGTTGIAVGMATDIPPHNLREVAQAAIALIDQPKTTLDQLLDIVQGPDYPTE
                     AEIITSRAEIRKIYENGRGSVRMRAVWKKEDGAVVISALPHQVSGARVLEQIAAQMRN
                     KKLPMVDDLRDESDHENPTRLVIVPRSNRVDMDQVMNHLFATTDLEKSYRINLNMIGL
                     DGRPAVKNLLEILSEWLVFRRDTVRRRLNYRLEKVLKRLHILEGLLVAFLNIDEVIEI
                     IRNEDEPKPALMSRFGLTETQAEAILELKLRHLAKLEEMKIRGEQSELEKERDQLQGI
                     LASERKMNNLLKKELQADAQAYGDERRSPLQEREEAKAMSEHDMLPSEPVTIVLSQMG
                     WVRSAKGHDIDAPGLNYKAGDSFKAAVKGKSNQPVVFVDSTGRSYAIDPITLPSARGQ
                     GEPLTGKLTLPPGATVDHMLMESDDQKLLMASDAGYGFVCTFNDLVARNRAGKALITL
                     PENAHVMPPVVIEDASDMLLAITQAGRMLMFPVSDLPQLSKGKGNKIINIPSAEAARG
                     EDGLAQLYVLPPQSTLTIHVGKRKIKLRPEELQKVAGERGRRGTLMRGLQRIDRVEID
                     SPRRASSGDSEE"
     gene            complement(29244..30851)
                     /gene="ygiS"
                     /locus_tag="ACHKBL_000849"
     CDS             complement(29244..30851)
                     /gene="ygiS"
                     /locus_tag="ACHKBL_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFO5817762.1"
                     /translation="MYTRNLLWLVSLVSAAPLYAADVPANTPLAPQQVFRYNNHSDPG
                     TLDPQKVEENTAAQIVLDLFEGLVWMDGEGQVQPAQAERWEILDGGKRYIFHLRSGLQ
                     WSDGQPLTAEDFVLGWQRAVDPKTASPFAGYLAQAHINNAAAIVAGKADVTSLGIKAT
                     DDRTLEVTLEQPVPWFTTMLAWPTLFPVPHHVIAKHGDSWSKPENMVYNGAFVLDKWV
                     VNEKITARKNPKYRDAQHTVLQQVEYLALDNSVTGYNRYRAGEVDLTWVPAQQIPAIE
                     KSLPGELRIIPRLNSEYYNFNLEKPPFNDVRVRRALYLTVDRQLIAQKVLGLRTPATT
                     LTPPEVKGFSATTFDELQKPMSERVAMAKALLKQAGYDASHPLRFELFYNKYDLHEKT
                     AIALSSEWKKWLGAQVTLRTMEWKTYLDARRAGDFMLSRQSWDATYNDASSFLNTLKS
                     DSEENVGHWKNAQYDALLNQATQITDATKRNALYQQAEVIINQQAPLIPIYYQPLIKL
                     LKPYVGGFPLHNPQDYVYSKELYIKAH"
     gene            complement(30960..31442)
                     /gene="ygiV"
                     /locus_tag="ACHKBL_000850"
     CDS             complement(30960..31442)
                     /gene="ygiV"
                     /locus_tag="ACHKBL_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFO5817763.1"
                     /translation="MTNLTLDVNIIDFPSIPVAMLPHRCSPELLNYSVAKFIMWRKET
                     GLSPVNQSQTFGVAWDDPATTAPEAFRFDICGSVSEPIPDNRYGVSNGELTGGRYAVA
                     RHVGELDDISHTIWGIIRHWLPASGEKMRKAPILFHYTNLAEGMTERRLETDIYVPLA
                     "
     gene            complement(31495..31887)
                     /gene="ygiW"
                     /locus_tag="ACHKBL_000851"
     CDS             complement(31495..31887)
                     /gene="ygiW"
                     /locus_tag="ACHKBL_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB fold stress tolerance protein YgiW"
                     /protein_id="HFO5817764.1"
                     /translation="MKKFAAVIAVMALCSAPVMAAEQGGFSGPSATQSQAGGFQGPNG
                     SVTTVESAKSLRDDTWVTLRGNIVERISDDLYVFKDASGTINVDIDHKRWNGVTVTPK
                     DTVEIQGEVDKDWNSVEIDVKQIRKVNP"
     gene            32039..32698
                     /gene="qseB"
                     /locus_tag="ACHKBL_000852"
     CDS             32039..32698
                     /gene="qseB"
                     /locus_tag="ACHKBL_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum sensing response regulator transcription
                     factor QseB"
                     /protein_id="HFO5817765.1"
                     /translation="MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAP
                     YDAVILDLTLPGMDGRDILREWREKGQREPVLILTARDALAERVEGLRLGADDYLCKP
                     FALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGDPLTLKPKEFALLEL
                     LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGE
                     K"
     gene            32695..34044
                     /gene="qseC"
                     /locus_tag="ACHKBL_000853"
     CDS             32695..34044
                     /gene="qseC"
                     /locus_tag="ACHKBL_000853"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quorum sensing histidine kinase QseC"
                     /protein_id="HFO5817766.1"
                     /translation="MKFTQRLSLRVRLTLIFLILASVTWLLSSFVAWKQTTDNVDELF
                     DTQLMLFAKRLSTLDLNEINAADRMAQTPNKLKHGHVDDDALTFAIFTHDGRMVLNDG
                     DNGEDIPYSYQREGFADGQLVGEDDPWRFVWMTSPDGKYRIVVGQEWEYREDMALAIV
                     AGQLIPWLVALPIMLIIMMVLLGRELAPLNKLALALRMRDPDSEKPLNATGVPSEVRP
                     LVESLNQLFARTHTMMVRERRFTSDAAHELRSPLTALKVQTEVAQLSDDDPQARKKAL
                     LQLHSGIDRATRLVDQLLTLSRLDSLDNLQDVAEIPLEDLLQSSVMDIYHTAQQANID
                     VRLTLNANGIKRTGQPLLLSLLVRNLLDNAVRYSPQGSVVDVTLNADNFIVRDNGPGV
                     TPEALARIGERFYRPPGQTATGSGLGLSIVQRIAKLHDMNVEFGNAEQGGFEAKVSW"
     gene            complement(34090..>34170)
                     /locus_tag="ACHKBL_000854"
     CDS             complement(34090..>34170)
                     /locus_tag="ACHKBL_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2645 domain-containing protein"
                     /protein_id="HFO5817767.1"
                     /translation="WLYVISLVICLVCGWWLFWGRFIFCY"
BASE COUNT         8338 a         9166 c         8660 g         8006 t
ORIGIN      
        1 tcagtacggg gttgctacaa catgtattac tctttcacca caattagcgg ttcaatatca
       61 ctctcttttt tgatgaccag tgattcatca ccgcgcaaac acgtcccacc gtagttgccg
      121 ccaacggtga aggtacatac ctgaatgtat ttaccgtcca ctttcggcaa acaccacagt
      181 tgctgataga tgtttttctg ctcggcaaat ttaccgctgg ttttgtccag cacctcttca
      241 tgatggctga cgaggtcgat gttactgcca cagcgaccgg cgatcggttt cactgcataa
      301 ccggttttca ccagttcatc attcacggag aaatcggtat ccagcaggta acgatggtgc
      361 gggaacagcg accagaggat cggcagaatc gctttgttgc cggggatcac tgtccacaga
      421 ggttcaaaga ccagcacttc cgggcgcagc aatacgtcga taagacgcac ttcattttgc
      481 ggatgaccgg tacggatcgg caccgcagca aactcacggt cgctaacttc acgaatctga
      541 tcaaacgcgg tttcccacgc ccaggttttc cacacgcagt taaccagtcg cccttcccca
      601 tcaatcagtt gcccggcagc atcccagccc agttcatcca gcccgcgcaa gatacgcgtt
      661 tcaaagcccg cctggtgcag cgcctgctcc ataaactgcg cgtgatagtt ttcctcgata
      721 tctttgtcct gcatgatatg gacaaacgga cgtgcacgac tgtgtttcca ggcaccagcc
      781 agttcgttaa tcagcccttc cgccggattg aagccgttgc ctttatagcc ctgctccgcc
      841 caacgttcga ggatcaagcc agcttcggta tgacaggagg cggagtcggc gttgtattcg
      901 tagaccttca ggccacgttc gtccatacag aaatccatac gaccggtgat catatggtga
      961 cggcgacgct gccaggagag acgcaaacgt ggccagagga tttttgggat gtcgaacagc
     1021 gccagcaggt tgtcatcttt cagcaccttg tcggttgcgt gaagatacat caggtgcagt
     1081 tcgttggtgg ctttaatcag ctcctgctct gcgctctcgg taatggtgta gtaatgatac
     1141 ggatcctgat tgatcacctg accgttggcc tgcacatagg cgttttgtag cggatctttt
     1201 tcatccagcc atttaccgtc aaactggcct ttgttttcca ggcgcgctcc gctgattttc
     1261 aacagctcgc ctgcaatttc cggctgcggt aagctgtatt cagtatcttc cgtctggatc
     1321 atccagccga gaatggtggt gtcatcaaaa gtgtctttaa gggtatagca gccgttttcg
     1381 accaccatct ccagctcgcg cgtccactgt tgcccttgcg gtaacgggga atgaatcacg
     1441 ttctgttccg caatacggac tttgttgcca tgcaattggg taatgatggc gacatgacca
     1501 gtgtctttaa attcaccgcc tttatcccag ataagaagcg cacccgcgac cggcgcacgc
     1561 ggcgagccgt taggaaatgc ctgcaatggc aggatgttgt cattaaccac ttcacgcagg
     1621 aagcgcagcg agaaaatctc ccacgccata cccacgtcag tgaagaccac accgtaattc
     1681 agaaagagaa aacggcgagc aaattcaacg cattgccatt tgtggcccat atattcgtcg
     1741 tcgatatagc tacggaatac ggcgtcatct tcgtattcct gcggatcgag agaactgtaa
     1801 tctgaagagt agattgctac cccacctggg gcgtagccca ataatgtccc gaacggggca
     1861 tcctggctgg tcgttccttt gctcatcact ttacctttaa caatacaacc taagcagttg
     1921 gcgtaattgt tgtgctctga ttacctgctc agcgacatta accggacaga ggtcagacta
     1981 atcatacacc tcatcgcagc ggctggcgca aagggttaaa aaattcacat tctgtacagc
     2041 aagtgacctg ctacactgct tcaacactac cactcagaag gcaactcact atgacagaca
     2101 acacttatca gcccgcgaaa gtctggacgt gggacaaatc cgccggcggc gcgttcgcca
     2161 atatcaatcg cccggtttct ggtccgacgc atgaaaaaac gctgcccgtt ggcaaacacc
     2221 cattgcaact ttattcgctg ggaacgccga acggtcagaa agtaacgatt atgcttgagg
     2281 agctgctggc gctgggcgtt actggtgcag agtacgacgc ctggctgatt cgtattggcg
     2341 atggcgatca attctccagc ggctttgtcg aagtgaaccc aaactcgaag atcccggcgc
     2401 tgcgcgatca tacgcataat ccgccgatcc gcgtgtttga gtctggatcg attctgcttt
     2461 atctggcgga gaaatttggc tacttcctgc cgcaggatct ggcaaagcgt actgaaacga
     2521 tgaactggct gttctggcta cagggggctg caccgttcct cggcggtggt tttggtcact
     2581 tttaccatta cgcgccggtg aaaattgagt acgccatcaa tcgctttacc atggaagcca
     2641 agcgcctgct cgacgtgctg gataagcaac tggcgcagca taagtttgtt gcaggcgatg
     2701 agtacaccat tgcggatatg gcgatttggc cgtggtttgg caacgtggtg ttaggcggcg
     2761 tgtatgatgc cgcagagttt ctggatgcgg gaagttataa acacgtacaa cgctgggcga
     2821 aagaagtggg cgaacgtccg gcagtgaagc gtgggcgtat tgttaaccgc atcaacggac
     2881 cgctgaacga gcagttgcat gaacgccatg acgccagtga tttcgagacg aatacggaag
     2941 ataagcgtca ggggtaaggg ttggtattcg tcgcagcaag ccatccaggc cggataaggc
     3001 gttcacgccg catccggcaa tcgagcacaa tgcctgatgc gacgctgccg cgtcttatca
     3061 ggcctacaaa agctaatcat cacgcgctgg taatgtcgta atccatttgg cgcagcgcgt
     3121 ctaatgtggc tttggcttca tcttcgtcga tgatgctcat ggcaaatccg acgtgtacca
     3181 gtacccactg gcccagcaga tcggcagggt taccttcaca aatcagggcg atattcacgt
     3241 cgcgcttgat accacatact tcaacctgcg caagctggtg aatatcttca ccgacagcca
     3301 gcacctggcc tggaacgcca atacacattg ttaactccgg ttattcaact tcaatacttt
     3361 tgacgattag cgaatcgccg gtatcgacac gcaaccgctc gccatgacag atcggacact
     3421 gcgcatcgtg ctgatgaatc tccaccacct ggctgcaatc ccagcaccag gcctgggcgg
     3481 gtttatagac gatatgtaaa tcgcaccctt gcgccaccgt tccgtggcag acaatttcaa
     3541 aactaaaacg gacggcgctc tcctcaacgc aggagagcgc accaatttcc agccacacgg
     3601 cggtgacgcg cttaacatcg tgctgctccg cctgccgttg gataatttca acggcgctct
     3661 ggcaaagcga caactcatgc attttcgcca ctcctgcggc caaatagcag ggcacgacgc
     3721 cctgcgtgtg gtacatccgg attggtgact ggcagcgaaa ggagcattcg ggcgcagtca
     3781 tccgtcagcc gttggccctc ttcaatcgac atgctgtgcg aaagcggcga catcagcgag
     3841 caggagaggt attgcgaaac accatccagt tcaccaacag taaaggtcat agtgccatac
     3901 ggcagttgca gaccaatttt ttcactcact ttgcgcagcg gccagagttg atccgggccg
     3961 gggaagataa ctgcactcag catccacggg gtgatcacac accccgtcca ctgaccttcg
     4021 aacagcgtaa agtcagaaac atacaccggc attgaaggat gcagaaaaga gagatcatgc
     4081 atcgaacgcc gggcaatttc ttcaaaagct gcctgtactt gcgccttcgg ggaggtctgg
     4141 aaacctgcta tctcttcagt catgaatcgc ctcccgtggg atggcttcca cgcctgattc
     4201 acgcagcgca gccagaacct gctcaagcgc aggttcaatc atcgcttcga ccgtcggcgt
     4261 caagccaatg tgcggctcca gtgattccgg gatcacgccg accagggtca gctttttcgg
     4321 aaactcaccg gtgaagcgca gggccgacaa tacgtcggcc aggccaagct gatgcggaga
     4381 gattttgttg gtaaacaacg ccggaacttc ttcatctcgc aggatcatca tcgttcccgg
     4441 cgcgttcttt ttcgagacaa tggcatccgc aataatcaga tgatcgcgat ttgccatgtc
     4501 gccaagcagc tccattcccg ccgttccgcc atcgaggatc tcaacataat ccggcagaat
     4561 gtatcgttgc tctaacgctt caacaattcg cacaccgatg gcttcatcgg tcagcaaaat
     4621 attgccaacc cctaagacta aaatacgcat tacagaacct tcactgaaac cacttcgttg
     4681 ccgtcggcat ccactacgtg taccgcacag gccatgcacg ggtcgaagga gtgaatggta
     4741 cgcaccactt ccagcggttt attcggatcg gcaaccggtg tacccaccag cgactgctcg
     4801 taaggaccga cgtcatcatt gaagttacgc gggccagagt tccaggttga tggaacaact
     4861 gcctggtagt tactgataat accgtctttg atcaccatcc agtgagagag cataccgcgc
     4921 ggtgcttcaa ggaaaccaac gcctttgaac tcacctgtag ccggaatgtt cggcttcaca
     4981 aaggtggtgt gatcgccttt gccgatattg gtgatcagtg cactgtattg attttgcagg
     5041 atatcctgca attcgcagca gtgaacggta cgaccaataa tacggcccag cgtggagtgc
     5101 agctgcgcca cttccagcgt gttgccagtc agtttctgat aaatcgcaac gatttcattc
     5161 agtttgttct gggtagactc gcgtcctgcc gccagtttca ccagcatgtt agccagcgga
     5221 cccacttcta ccgttttgcc gtagaaagtc ggtgatttca cccaggaata tttaccgtcg
     5281 tcagaccaac catcataagc cggaatggtg gtaccttccc acggtgcctg cggcgcttcg
     5341 tctttatacc aggagtgctt cgcgctctcc tgaatgcctt taatcaggta ttcatcggag
     5401 tgggaagtga tcgggcgata cgaggacaga tccgcattct caatgtagcc gcccgggaac
     5461 aggaagctgc cgtttttgct gtcggtcggg aattccggca cgctcaggta gttcaccgca
     5521 cctttaccgc gcgtcagcca ttccgggtag aacgcggcga taactgcggt atcaaccttg
     5581 taaacctgct caacaaagtc gctcagttta tcgatgaaag acttgatgta catcaggcgc
     5641 tcaaggttca gcacgcccag accgtcgagg ttgatcgggt tagcgacccc acctactgcc
     5701 aggttctgaa tgtgcggcgt tttaccgccc agcagcgcca cgacgcggtt agcgtcacgc
     5761 tggcactcca gcgcctgcag gtagtgcgct accgcaatca ggttcacttc cggcggcagt
     5821 ttcatcgccg ggtgacccca gtagccattg gcgaaaatcc ccaactgacc gctggcaacc
     5881 agatctttaa tcttgttctg aactttggtg aactcttccg gactgttcag atgccaggtc
     5941 gaaacgcctt tcagcatttc agaggctttg gttgggtcag cttgcagtgc agaagtgatg
     6001 tccacccagt ccagcgccga gagctgatag aaatgaacga tatggtcatg cgtggtgtgc
     6061 gcagccagaa tgatgttacg gatgtattgc gcgttaaccg gaacgtcgat attcagtgca
     6121 ctttctgccg cacgaacgga agagagcgcg tgagtagtcg tacatacgcc acagatacgt
     6181 tgcacaatca tccatgcatc gcgcggatcg cggtttttca cgatctcttc catgccgcgc
     6241 cacatggtac cggaagccca tgctttcgaa acgacgccat tttcgatttc gcaatcgatg
     6301 cgtaaatgcc cctcaatacg ggttaccgga tcaatagtaa ttctctggct catgctttgc
     6361 tcgcctcatg acgattatga tcgttttgtt ttaaaggagg aagtatcggc agtagacgaa
     6421 tgagtacgat gtaagtgcaa atctcaatag ccacaaaacc aatagaaatc aacagttctt
     6481 cccaggtcgg gaagtaggcg taaccgccgc ccgggttgaa tgccaccagc gaataggtca
     6541 gacgccaggt tgcacaacct aacagcgcgc tcagtgctga caggaacagc atgcgggaat
     6601 cattacgcag cttcgccaca cgcagaacga ccagcgggaa gagcatcagc aggacttcaa
     6661 tccagaacat cgcggagtag aagtcaccgg caaacgctaa cgacagcttg tcgcgataga
     6721 taagctcgcc aaagcgcagc acgatgaaaa tcgccagcaa cacactgatg gtgttggtca
     6781 gcttgacgaa caggctcttt tcatctggac cattgccacg cagacccgcc tgcaccaacg
     6841 aaccttcaaa gatgacaatc gagaagccca tgatgaacgc cgtcagcagc gagaacagcg
     6901 gcaacatttc atagctctgc cacaacggat gcactttgta gcccgccgag atcatcagcg
     6961 accccattga agactggtgc atagttggca gcagcgcacc gagcgcgata atgaagaaca
     7021 tcaccttgtt cagacgctgt agcgacacct tccagcccaa tcgttcaaac aatgccggag
     7081 caaactccag tgccatcacg ccgatgtaga tggtcataca gaccgccgtc tcgaacagta
     7141 ccgagttcac gttgaagtga cccggaatgt agaagtacgg caggttccag tagcgaccaa
     7201 cgtcgatggt gatcgacaag ccacccagtg agtaaccgaa cagactcgcc aacagcgccg
     7261 gacgcaccag cggatggtat tgcccacggt taaagacgta taccgcccac gccagcgccc
     7321 agccgccaca ggcaaagccg gtgccgatca acaggtcaaa cgcgatccac acgccccacg
     7381 ggaagccgcc gttcaggtca gagacagagc ccagaccgaa caccagacgc ttcacaatca
     7441 ggagcataca gatgacgatt aacggtccaa aaatcatgac cggtttactg atgattttgc
     7501 cgcccagcgg ttgtggatca tgactcatga tcgtctcctc cgtcgtgatg gtcgtttttg
     7561 gtgttgcgac gaaccagcac ggttaagccc gccagcacag ccagtggtag catcatgcct
     7621 ttatacaggg tgtgttgaat attttcggaa cgcgcaccgg tagaaagatc gtccagtttc
     7681 ggcaggtcga gattttcgta aggcacaccg gtcagtacca gaacctgagt accgccgccc
     7741 tctttctcgc cgtacagatg cggataatat ttcggcaccg tatgcaggta agtgtcgcca
     7801 gatttcagcg tctgacgcgg atagtggtat tcgctgcctg gcttcagcgc cagacgtttt
     7861 ttcgcctcag ccatcagctc ttcacgcgta ccaaaaatca ccgcgcccgc cgggcacact
     7921 tctacgcagc caggcagacc gcctttatca agacgttcca cacctttctg gttgcacagt
     7981 tcacacttat gcagcgcacc aaatgggttg ttgtagtcgt acttcggcac gttgtatggg
     8041 caggcgacca tgcagtaacg gcagccagtg cacacatctt tgtcgtaatg gacaatgccg
     8101 gttttcggat cttttttcag cgcagagacc gggcacacag agacacaatt cggatcgacg
     8161 cagtgcatac actgtttctt aatgtacgcg tagccgttct cctcctggtc tttgttgacc
     8221 cccgagccgc tggtccacac ctgaatgatg ttattggtgt acggcgacag tttgtcgttg
     8281 ttcgaccagg tctgttcccc ttgcgggtta cgttcaggga aattgatatc ctgacacttg
     8341 gtgacgcaag cctggcagcc aacgcacaag gtcgagtcgt acaacatccc cagcgatccc
     8401 ggaattggcg ggcggttttc agcagccgca tgactgacag acggcagcgc gcccgtcagc
     8461 aatgccccgc aggaggctgc tttaataaaa ttacgtctgt tcacggttat tctccccgtg
     8521 agtcagcgtt atctttcttt tgctgacgac ccagttcacg caccgccatc acgctgacac
     8581 cggcaaccag cccaaccaca ccgccgagca aaccaatagc gcccgcagag acgttgccgc
     8641 cttctttagc gttaacgtcc ggtttctgtg aacgcggagt ctgattttcg acgttggcaa
     8701 gctgatggat gcctttatgg aagccgatac cttcttcgtt acagccatag cacgggtgac
     8761 caatcgctac cggccacacg ccgccaacgt cgcagaattg cagcgttgag cagttgccgt
     8821 atgtttctgg ccctttacag ccgaggtggt acaggcacca gccttcgcgg tggccttcat
     8881 caccgaactc tttggcaaaa cgaccagcat cgaagtgcgg gcgacgttcg cagtgttcgt
     8941 gaatcagacg gccataggcg aaggtcggac ggtttttgtc atccagtttc ggcggcttgc
     9001 cgtaagtgat gatgtgcgca acggtcgcga ggaagttgtg cgggttcggc gggcagcccg
     9061 gaatattgat aacggttttg cctggcagaa cttcttgcag gctgactgcg ccagttgggt
     9121 taactccagc ggcggcaaca ccgccccacg cagagcagga accgatagcg ataatggctg
     9181 ctgcgccttc cgccgctttg cggatgtgat ccacaatcgg ctcaccggca accatgcaat
     9241 aaataccgtt atcttttaat gggatggaac catccaccac taacacatac tgccctttgt
     9301 acttctcgag agcgttatgt ttgttctctt cgacctggtg accgaaagca gcagaaagca
     9361 cctcgtgata ctccagagag atagtctcca gcacgaggtt ttctaccgtt ggatgcgttg
     9421 cacgaagcag agattccgta caaccggtgc actcctgcgc gccaatccag ataactggcg
     9481 gacgctgcgg gttagtaacc gattcggcca tctctgcagc ggctttgcta cttaacccca
     9541 tggtggcggc taatgctgca caaagcttca tgaaatcacg acggttaatg ccgtgagaat
     9601 ggatgagggt gttatctcca gtcatttata gttattccgt tgcgaagacc tggcatatat
     9661 tttgcctcaa tcgcaaaatc aataatgcga tcgatgcgcc atttaccaca catttattat
     9721 ggttatcgat atcatgatac tgcggcgggg cgctaaaacg aagggaagaa gcattagtgt
     9781 aattaattaa agcaagataa tacgtatgtt tgatcaattt tcgaggcgat aaccggccat
     9841 aaaaaagcgc aacgtagaac aggaattatt ctcacgaaag aaatccatta ccatctcttt
     9901 atccagtcca tagcggcgcg tcagaatacc cgcttcccag gcgctaagct ggcgatctcc
     9961 ggtcttttcg tacatccggt gtgagtagcc taatacaaaa ccgcgtttat aatcggcaca
    10021 gtattttgtc acattaaccg cactgtcggc atgcgttgct tttaatcccg ccattaagcc
    10081 tttaccaaaa tggttattca tcgttcacct cattcgctat atattgccgt atatagcgat
    10141 attttaggca aagaacgaca tttgcgcacg ctattttttc atgcttgttt aagacgtaat
    10201 cctgtaagag ataaacccat caggagagat atttatcgaa ccatttcagc gtcctttgcc
    10261 aggcaagatc ggcggcagat ttgtcataac ggggcgtgga atcattatgg aatccgtgat
    10321 taacccccgg atatatatac gcctcataga ccttattatt ggctttcaac gccgcctcgt
    10381 aagcaggcca gccctcgttg attcgggtgt ccagttccgc gtagtgcaac agtaaaggcg
    10441 cttcaatctt cgccacatcg gcagtgggtg cctgacgacc ataaaacggc accgcgcagg
    10501 ccagttccgg gtaagcgacc gctgccgcgt tcgatacgcc accgccatag caaaatccag
    10561 taatacccac tttgcctgtc gcttgcggat agctttgcat aaactcaatc gcggcaaaga
    10621 aatcattcat cagtttggtt ggatcaacct gctgttgcag ctcacgacct ttatcatcat
    10681 tacccggata acctccaacg gaacttaagc cgtcaggtgc cagggcgata taccccgcct
    10741 tcgccactcg ccgcgtcaca tcttcgatat acggattcag ccctcgattc tcatgcacca
    10801 ctaccacggc tggcgttttg ccgctcattt ttgcgggctt caccagataa ccccgtacct
    10861 cgccgtgacc atttggcgag ggatacgtga tgtactctgc aacaatttcc gggtcggtaa
    10921 actctacctg agtcgccagc gcataatttg gcttgagcaa atcaaacagc gctaatgccg
    10981 tcatcccgcc caccgcatac ttcgccgcaa ggttgaggaa ctcacgtttc gagattttcc
    11041 cgtgagcgta atagtcgtag taatccagca actcttgtgg gaaatctttg gcggttaaac
    11101 gcggcatcgt tgcactcctc aactggtgtt tttttaagca aagcataagc gcgtgttttt
    11161 gcccagtttt tcgtcactct gtgagccaga ctacgggata cgcgctggcg aatcgctaaa
    11221 ctagaaacat tgtttcgaaa ttgaacgatg gaaaggagag gtcatggtct ggttagcgaa
    11281 tcccgaacgt tacgggcaga tgcagtaccg ctattgcgga aaaagtggtt tacgcctgcc
    11341 cgcgttatcg ctcggtttat ggcacaattt cggtcacgtt aacgcgctgg aatcacagcg
    11401 tgcgatcctg cgtaaagcgt ttgatttagg cattacgcac tttgatttag ccaacaatta
    11461 cgggccgcct ccaggaagcg cagaagagaa ctttggtcgc ctgctgcgcg aggattttgc
    11521 cgcttatcgc gatgaactga ttatctctac caaagccgga tacgatatgt ggcccggccc
    11581 gtacggctct ggcggctcac gtaaatacct gctcgccagc ctcgaccaaa gcctgaagcg
    11641 tatggggctt gagtatgtcg atatctttta ctctcatcgc gtcgatgaaa atacgccgat
    11701 ggaagaaacc gcctctgcgc tggctcatgc ggtacaaagc ggtaaggcgc tgtatgtcgg
    11761 gatctcctct tactcgccag agcggacgca aaaaatggtt gagttgctgc acgagtggaa
    11821 aattccgctg ttaattcatc aaccttcgta caatttactg aaccgctggg tggataaaag
    11881 cggcctgctg gataccctgc aaaataacgg cgtgggctgc attgccttta ctccactggc
    11941 tcagggattg ctgaccggaa aatatctcaa cgctattccg caagattcac ggatgcatcg
    12001 tgaagggaat aaagttcgtg gtctgacgcc gaaaatgctc accgaagcca acctcaacag
    12061 cctgcgctta ttgcatgaaa tggcacagca gcgtggacaa tcaatggcgc aaatggcgtt
    12121 aagctggttg ctgaaagatg agcgagtgac gtcggtattg attggagcca gccgcgcgga
    12181 gcaacttgag gagaacgtgc aggcgctgaa taatctgaca tttagcaccg aggagctggc
    12241 gcagattgat cagcatatcg ccgatggaga gctgaatctg tggcaggcgt cttccgataa
    12301 atgacctgtt aataacgggc gcgagaaccg cgcccgaatc agatcagtga ttatgacgag
    12361 tcagtcggtc aagatagccc atcacaaatg cagagagcac aaacgtcaga tggataatga
    12421 cgtaccacat cagtttgtta tcagggacat ttttcgcatc cataaagacg cgcagtaagt
    12481 gaatggaaga aattgccaca atcgacgctg ctactttgtt tttcagcgac gttgcgtcca
    12541 ttttccccag ccagttcagc ttctctttgt tctcagagat atcgagttgt gagacgaaat
    12601 tctcgtaacc ggaaaacatc accatcacca gcaaaccgcc aaccagcgtc atatccacca
    12661 gcgacagcaa cacgaggatc aaatctgatt ccgccatcga gaagatattc ggcagtacgt
    12721 gaataatctc ctggaagaac ttcagcgcca gggcaactaa cgccagcgaa aggccaaagt
    12781 acacgggggc aagcagccag cgagaagcat acattgcatt ttcaagaaaa cgttccatac
    12841 attcctgtcg tgtagttaaa acgggcaaca gtatatcgca attaagtaaa cagcacgcaa
    12901 cagcaaatca acatatacgt taaacctttg tcgcataatt ctgagggtta gggatcttcc
    12961 tggttccctt atcaacgcat cgagagcgtc tacccttaaa ggacgttgaa cctgaaggga
    13021 gaaaaatgat gtctcattta aaagacccga ccacgcagta ttacactggt gaatatccca
    13081 aacagaaaca accgacgcca ggcatccagg cgaagatgac accggtaccg gattgcggcg
    13141 agaaaaccta tgttggtagc ggtcgcctga aagatcgtaa agcactggtg acagggggcg
    13201 attccggaat cggtcgcgct gccgccattg cttacgcgcg tgaaggggct gacgtggcga
    13261 tcagttatct tcccgtggaa gaagaagacg ctcaggatgt caaaaagatc attgaagaat
    13321 gcggacgcaa agccgttctg ctgccaggcg atttaagcga tgagaaattc gcccgttcgc
    13381 tggttcacga agcacacaag gcgttaggcg ggctggatat tatggcgctg gtcgccggga
    13441 aacaggttgc cattactgat attgcagacc tcaccagcga acagtttcaa aagacctttg
    13501 ccattaacgt tttcgcgctg ttctggctaa cccaggaagc gatccccctg ctaccgaaag
    13561 gtgccagtat catcaccact tcgtcaatcc aggcatacca gccaagcccg catttactgg
    13621 actatgcggc tacgaaggcg gcaattctga actacagccg tggcctggca aaacaggtcg
    13681 cggagaaagg tattcgggtg aatattgtcg cgccaggccc gatctggaca gcactgcaaa
    13741 tttccggcgg acaaacgcag gataagatcc cgcagtttgg tcagaaaacg ccgatgaagc
    13801 gtgcggggca accggcggaa ctggcccctg tatatgttta tctggcaagt caggaggcga
    13861 gctacgtcac cgcagaagtg catggcgtgt gcggcggcga gcatttaggc taaaaaaatg
    13921 cccggttgtg aaaagcaacc gggcatcatt tttatttcgc tttggcagtt tcttcgccga
    13981 ccagacctat cttcaggtaa cccgcctgat gcagcgtatc cattaccttc atcaacgtct
    14041 cgtaatcgac ggttttatcc gctcggaaga agacggtggt gtctttcttg ccttcggtta
    14101 acgcattcaa cgccgtaatc attgtttcat cggtgaccgg atcgttaccg ataaacatcg
    14161 agttgtctgc cttcaccgac agataaaccg gtttttccgg gcgcggctgc ggcgtgctgg
    14221 tagaagcagg caagttcacc ttcacatcta ccgtcgctaa cggtgccgcc accataaaga
    14281 taatcagcag aaccaacatc acgtcgataa acggcgtcac gttgatatca tgcatttcgc
    14341 cgttatcgtc gaggttttcg ttaagatgca ttgccatcgg acattatcct gcgcgtaatt
    14401 tttgtgcgac acgaaccgga tgcgcagcgg cgctggcttc cagatccagg tcacggcttt
    14461 gcagcaacaa tacctgcgct gcaacatcac ccagcatcgc tttaaagccg ccaatctggc
    14521 gtgcaaatac gttatagata acgaccgccg gaatcgctgc cacgaggccg attgccgttg
    14581 ctaacagagc ttctgcgata cccggcgcaa cgactgccag gttagtagtc tgcgtttgcg
    14641 cgataccaat aaagctgttc atgatgcccc agaccgtacc aaacagacca acgaacggcg
    14701 aaatcgcgcc gatggttgcc agatagccgt taccacggcc catttgacga cctactgcgg
    14761 cgacccgacg ctccagacgg aagctggtac gttctttaat accttcgtta tcgtcgctgc
    14821 cttctgacag ttccagctca ttctgcgctt cattgagcaa atgcaggctc aagcttttgc
    14881 taccaaaatc agcggcgata tcgttggcct ggtttaagga acgcgcttca gccagcagtt
    14941 gctgctcgcg cttaagacga cgcttctgat tgaagaactc cacgctctta ctgaagaaga
    15001 ttgcccaggt gactacggag gccaaaataa gcccaatcat cacgcactta acgacaatat
    15061 cggcgtgctg atacataccc cagacggaga ggtccgtctg cattaaatta ttacccactg
    15121 tgtatctcca ggacgcaagt cacaaaatct gcgcataata atatcaaaac gacgtcgaat
    15181 tgatagtcgt tctcattact atttgcatac tgccgtacct ttgctttctt ttccttgcgt
    15241 ttacgcagta aaaaagtcac cagcacgcca tttgcgaaaa ttttctgctt tatgccaatt
    15301 cttcaggatg cgcccgcgaa tattcatgct agtttagaca tccagacgta taaaaacagg
    15361 aatcccgaca tggcggacaa aaagcttgat actcaactgg tgaatgcagg acgcagcaaa
    15421 aaatacacgc tcggtgcggt aaatagcgtg attcagcgcg cgtcttcgct ggtctttgac
    15481 agtgtggaag ccaaaaaaca cgcgacacgt aatcgcgcca atggcgagtt gttctatgga
    15541 cggcgtggaa cgttaaccca tttctcctta caacaagcga tgtgtgaact ggaaggtggc
    15601 gcaggctgtg tgctatttcc ctgcggggcg gcagcggttg ctaattccat tcttgctttt
    15661 gtcgaacagg gcgatcatgt gttgatgacc aacaccgcct atgaaccgag tcaggatttc
    15721 tgtagcaaaa tcctcagcaa actgggcgta acgacatcgt ggtttgatcc gctgattggt
    15781 gccgatatcg ttaagcatct gctgccaaac actaaaatcg tgtttctgga atcgccaggt
    15841 tccatcacca tggaagtcca cgatgttccg gcgattgttg ccgccgtacg cagtgtggtc
    15901 ccggatgcca tcattatgat cgacaacacc tgggcagccg gtgtgctgtt taaggcgctg
    15961 gattttggca tcgatgtttc tattcaggcc gccaccaagt atctggttgg gcattcagat
    16021 gcgatgattg gcactgcggt gtgcaatgcc cgttgctggg agcagctgcg agagaatgcc
    16081 tatctgatgg gccagatggt cgatgccgat accgcctata taaccagccg tggcctgcgc
    16141 acattaggtg tgcgtttgcg ccaacatcat gaaagcagtc tgaaagtggc tgaatggctg
    16201 gcagaacatc cccaagtagc gcgagttaac caccctgccc tgcctggcag taaaggccac
    16261 gaattctgga aacgagactt tacaggcagc agcgggctat tttcctttgt gcttaagaaa
    16321 aaactcaatg atgaagagct ggcgaactat ctggataact tcagtttatt cagcatggcc
    16381 tactcgtggg gcgggtatga atcgttgatc ctggcaaatc aaccagaaca tatcgccgct
    16441 attcgcccac aaggcgagat cgattttagc ggaaccttga ttcgcctgca tattggtctg
    16501 gaagatgtcg acgatctgat tgccgatctg gacgccggtt ttgcgcgaat tgtataacat
    16561 tgccactttt ggacaatttt gcagacattt tattgtgaaa agtcttaaat tgttgcgtcc
    16621 gggatcaagg cgtcccggac gattcaggag tacaataggc agataaaggc ttaaacgctg
    16681 ttccacagga aagtccatgg ctgttattca agatatcatc gctgcgctct ggcaacacga
    16741 ctttgccgcg ctggcggacc ctcatattgt tagcgttgtt tactttgtta tgtttgccac
    16801 gctgttttta gaaaacggct tgctgcccgc ctcatttttg ccaggcgaca gcttgttgat
    16861 actggcaggc gcattgattg cccagggggt tatggatttt ctgcctacga ttgcgattct
    16921 gaccgccgca gcaagtctgg gctgctggct aagttatatt caggggcgct ggttagggaa
    16981 taccaaaacg gtgaaaggct ggctggcaca gcttcctgct aaatatcacc agcgcgccac
    17041 ctgcatgttt gaccgccacg gtctgctggc gctgctggct ggacgttttc ttgcatttgt
    17101 ccgtacgctg ctgccaacca tggcgggaat ttccggtctg ccaaaccgcc gcttccagtt
    17161 tttcaactgg ttaagcggat tgctgtgggt cagcgtggta accagtttcg gctatgcctt
    17221 aagtatgatt ccgttcgtta aacgccatga agatcaggta atgacgttcc tgatgatcct
    17281 gccaattgcc ttgttaaccg ctggcttgtt gggcacgctg tttgtggtga ttaaaaaaaa
    17341 atactgtaac gcctgacgat tttccccatt cccggtagct gtaccgggaa cgtatttaat
    17401 tcccctgcat cgcccgcatt cttgccgcat cttcccccgg cgtcacaccg aagtaacgtt
    17461 taaactcacg gctaaattgc gatgcgcttt catagccgac gcgcatcgcc gccgcgctgg
    17521 ccttcatgcc gtcgtggatg atcatcatcc gcgccttatg cagacggtaa ttcttcaaat
    17581 actgcaacgg cgaggtgctg gtgacagact taaaattatg gtggaacgcc gatacgctca
    17641 tgttggcttc tgccgccagt tgctcgacgc tcaggttttc ggtgtattta ttctcaatcc
    17701 gtttcagcac gcgactaatc aggctgaagt gagtctggcg gctgaccagt gccagtaacg
    17761 cgccgccgca aggtccggtc agcacgtagt acagaatttc gcggatgatc tgtttgccga
    17821 gaatacgcgc atccagcggt cgctccatca catccagtaa ccgctccgcc gcgcataaaa
    17881 tctcttctga taacgtggca gagttaatcc cgctggctgc catcgacggc tggaaatgct
    17941 catcttcgcc aatgtccatc aacagttcct gtaactgcaa aatatcgaca ttgagacgca
    18001 accctgccag cggcacctct gacgtggcat aggtttcgca ctcaaacggc aacggcaccg
    18061 tcagcaggag atattcatta gcgtcataac gaaacacgcg ttcattgata taaccgattt
    18121 tatgtccgga aaagagaatt atgatgccag gctcgtacat caccggtgtg cgtgcgaaag
    18181 gcgtctcgcc atacaacaaa cgcacatcgg gcaacagctc tgacaaacta ttttctttat
    18241 ttttcagttt attaacttta tccgccagca agcggcaaat ctcttcacgt ttcatatcgc
    18301 gtaatttctt aggaataatg cggcaatttg attgtgcgca attttgtagc atttctccag
    18361 cactctggag aaataggcaa gacattggca gaaatgagca ttgagaggta cggcgctggc
    18421 gaccacaatg aaaaacatca ggcagaccgt tctctgccct catattgacc cagcaaaggg
    18481 agcaagtaat gaacaacttt aatctgcaca ccccaacccg cattcttttt ggtaaaggcg
    18541 caatcgctgg tttacgcgaa caaattcctc acgatgctcg cgtattgatt acctacggcg
    18601 gcggcagcgt gaaaaaaacc ggcgttctcg atcaagttct ggatgccctg aaaggcatgg
    18661 acgtgctgga atttggcggt attgagccaa acccggctta tgaaacgctg atgaacgccg
    18721 tgaaactggt tcgcgaacaa aaagtgactt tcctgctggc ggttggcggc ggttctgtac
    18781 tggacggcac caaatttatc gccgcagcgg ctaactatcc ggaaaatatc gatccgtggc
    18841 acattctgca aacgggcggt aaagagatta aaagcgccat cccgatgggc tgtgtgctga
    18901 cgctgccagc aaccggttca gaatccaacg ccggcgcggt gatctcccgt aaaaccacag
    18961 gcgacaagca ggcgttccat tctgcccatg ttcagccggt atttgccgtg ctcgatccgg
    19021 tttataccta caccctgccg ccgcgtcagg tggctaacgg tgtagtggac gcttttgtac
    19081 acaccgtgga acagtatgtt actaaaccgg ttgatgccaa aattcaggac cgtttcgcag
    19141 aaggcatttt gttgacgctg atcgaagatg gtccgaaagc cctgaaagag ccagaaaact
    19201 acgatgtgcg cgccaacgtt atgtgggcgg cgactcaggc gctgaacggt ttgatcggcg
    19261 ctggcgtacc gcaggactgg gcaacgcata tgctgggcca cgaactgact gcgatgcacg
    19321 gtctggatca cgcgcaaaca ctggctatcg tcctgcctgc actgtggaat gaaaaacgcg
    19381 ataccaagcg cgctaagctg ctgcaatatg ctgaacgcgt ctggaacatc actgaaggtt
    19441 cagacgatga gcgtattgac gccgcgattg ccgcaacccg caatttcttt gagcaattag
    19501 gcgtgccgac ccacctctcc gactacggtc tggacggcag ctccatcccg gctttgctga
    19561 aaaaactgga agagcacggc atgacccaac tgggcgaaaa tcatgacatt acgctggatg
    19621 tcagccgccg tatatacgaa gccgcccgct aagcttttta cgcctcaaac tttcgttttc
    19681 gggcatttcg tccagactta agttcacaac acctcaccgg agcctgctcc ggtgagttca
    19741 tataaaggag gaacgtatgg ctaatccaac cgttattaag ctacaggatg gcaatgtcat
    19801 gccccagctg ggactgggcg tctggcaagc aagtaatgag gaagtaatca ccgccattca
    19861 aaaagcgtta gaagtgggtt atcgctcgat tgataccgcc gcggcctaca agaacgaaga
    19921 aggtgtcggc aaagccctga aaaatgcctc agtcaacaga gaagaactgt tcatcaccac
    19981 taagctgtgg aacgacgacc acaagcgccc ccgcgaagcc ctgctcgaca gcctgaaaaa
    20041 actccagctt gattatatcg acctctactt aatgcactgg cccgttcccg ctatcgacca
    20101 ttatgtcgaa gcatggaagg gcatgatcga attgcaaaaa gagggattaa tcaaaagcat
    20161 tggcgtgtgc aacttccaga tccatcacct gcaacgcctg attgatgaaa ctggcgtgac
    20221 gcctgttatt aatcagatcg aacttcaccc gctgatgcaa caacgccagc tacatgcctg
    20281 gaacgcgaca cacaaaatcc agaccgaatc ctggagtcca ttagcgcaag gtgggaaagg
    20341 tgttttcgat cagaaagtca ttcgcgatct ggcagataaa tacggcaaaa ccccggcgca
    20401 gattgttatc cgctggcatc tggatagcgg cctggtggtg atcccaaaat cggtcacacc
    20461 ttcacgcatt gtcgaaaact ttgatgtctg ggatttccgt ctcgacaaag acgaacttgg
    20521 cgaaattgca aaactcgatc agggcaagcg tctcggcccc gatcctgacc agttcggcgg
    20581 ctaacgtgca aattctcccg gtggcggtaa tgttccgcta ccggactttt cagaaatcat
    20641 ttattcccct cgcgtcctgc ccattgttac tcttccttgt tcaggaatgc caaatataag
    20701 gacatcatca tgcagagccg gaagctctta aaggaacaac tcatctacac ccgggataaa
    20761 cgcaacggag aggtgaaaaa cagatgaaaa taatacttct gtttttagca gccctggcaa
    20821 gttttaccgt acacgcgcag cctccttcac agaccgtaga acaaacagtc cggcagattt
    20881 atcagaacta taaatcagat gccagtacgc cttattttgg tgaaaccgga gagcgagcga
    20941 taacttctgc gcgtattcaa caggcgctta ccctgaacga caatcttacg ctgccgggca
    21001 atattggctg gctggattat gatccggttt gtgattgtca ggattttggc gatctggtgt
    21061 tagaaagcgt agcgataacc caacctgacg ccgatcatgc cgatgccgtt gtgcgctttc
    21121 gtatctttaa agacgataaa gaaaagacca cgcagacact gaaaatggtg gcggaaaatg
    21181 gtcgttgggt cattgacgat attgtcagca atcatggcag cgtcttacaa gcagttaata
    21241 gcgagaatga aaaaacgctg gccgctatag cttcgttgca aaaagaacag ccggaagcct
    21301 ttgttgccga actctttgaa catattgctg attatagctg gccgtggacg tgggtggttt
    21361 ccgactctta ccgccaggcg gtcaatgcct tctataaaac caccttcaag actaccaaca
    21421 atcccgatga agatatgcga atagaacggc aatttattta cgacaatccg atctgttttg
    21481 gcgaagagtc gctattttca cgcgttgatg aaattcgagt cctggagaaa accgccgatt
    21541 ccgcccgcat tcatgttcgt tttacgctga ccaatggcaa caatgaagag caagaactgg
    21601 ttttacagcg gcgcgaaggt aagtgggaaa tcgctgattt tatccgcccg aatagcggta
    21661 gcctacttaa gcagattgag gcaaaaaccg ccgccagatt aaagcaatga gctgagttaa
    21721 ataacaatta gccggaacaa taaataaaag ggaacactat atgaaaacga ttttcaccgt
    21781 gggagctgtt gttctggcaa cctgcttgct cagtggctgc gtcaatgagc aaaaggtcaa
    21841 tcagctggcg agcaatgtgc aaacattaaa tgccaaaatc gcccggcttg agcaggatat
    21901 gaaagcatta cgcccacaaa tctatgctgc caaatccgac gctaacagag ccaatacgcg
    21961 tcttgatgct caggactatt ttgattgcct gcgctgcttg cgtatgtacg cagaatgata
    22021 aaaaaattcc cggcagcatt ctgttaccgg ggatattttt taacgtccaa ccgccgcttt
    22081 agggcgtttc ttcgcaccag cattctgcag gcgagattgc gtagacgacg ctttttttgc
    22141 cgtagcaggc gtctgacgct gggtcgccat cggcgtatgt ttagtcaacg ccggacgggt
    22201 attgcggttc tggcgacgag cctcacgcat ctcttcaatg gttggcgcag gcactaagca
    22261 atcgcgacgg ctgccaatca gatgcttttt gcccatcgct tccagcgcct ggcggattaa
    22321 cggccagttt gccggatcgt ggtaacgcaa caacgcttta tgcaggcgac gctgcttgtc
    22381 gcccttcggt acaaagacgt cttcactttt ataaccaatc ttcgccagcg ggtttttgcc
    22441 ggtgtaatac atagtggtcg agttagccag cggcgacggg tagaagttct gtacctggtc
    22501 gaggcggaag cggtgctttt tcagccacag cgccagattc accatatctt catcgcgcgt
    22561 acccgggtgc gcggagatga aatacgggat cagatactgt tctttacctg cctgtttcga
    22621 gtaagtatcg aacagctctt taaagcggtc atagctgccc atgcccggct tcatcatctt
    22681 cgataacggc ccttcttcgg tatgttccgg ggcaatcttc agataaccgc caacgtgatg
    22741 ggtcgccagc tctttgatat agcgcggatc ttctacggcg atgtcataac gcacaccaga
    22801 ggcaatcagg atctttttaa tgcctttcag atcgcgcgca cggcgataga ggttaatcgt
    22861 cggttcgtgg ttagtgtcca tatgcggaca aatatccgga taaacgcacg acaaacgacg
    22921 gcaggtttgc tcagcgcgtg gcgatttgca acgcaacata tacatgttgg cagtaggccc
    22981 accgagatcg gaaatcacac ccgtaaaacc tggaacagtg tcgcggatcg cttcgatctc
    23041 attaataatc gaatcttcgg aacggctctg aataatgcgc ccttcgtgct cggtaataga
    23101 acagaaagag cagccgccaa agcagccacg cataatgttg accgagaaac gaatcatttc
    23161 gtaagccgga attcgggcat taccgtaagc cggatgcggc acgcgcttgt acggcagcgc
    23221 aaagacgcta tccatctctt cggtagaaag cggaatagca ggcgggttga tccatacata
    23281 gcggtcgcca tgtttttgca tcaatgcgcg ggcgcagcct gggttagttt cgtggtgaag
    23341 aatgcgcgaa gcatgggcgt acagcacttt atcgcccttc actttctcga aagaaggcag
    23401 caacacgtag gttttttccc acggtttcgg gcgcggcggc tgcacggtta cggctttcgc
    23461 ttcctgcttt ttcggcgcaa caggtttgtt atccgcgcac ggcagatctt cgccatacgg
    23521 atgcgggatc gggtcgattt tcccaggagt atcaagacgg gtggaatcca cgccgctcca
    23581 gcctggcaac gcctctttta cgataatcgc ggtattacgc acatcgcgga tttcactaat
    23641 cggctcgccc atcgccagac gatgcgccac ttccaccagc ggacgctcac cgttaccgaa
    23701 catcagcatg tcggctttcg aatccaccag cacggaacgg cgcacggtat cggaccagta
    23761 atcataatgc gcggtacggc gcagacttgc ctcaataccg ccgaggatca ccggtacatc
    23821 tttccacgcc tctttacaac gctgggtata aaccagtgtg gcgcgatccg gtcgcttacc
    23881 cgcgacgtta tccggcgtgt aggcatcgtc atgacgtaaa cggcgatcgg cggtatagcg
    23941 gttgatcatc gagtccatgt tgccagcagt aacaccgaaa aacagattcg gtttacccag
    24001 acgcataaag tcgtctttgc tgctccagtc cggttgggca atgatcccga cacgaaagcc
    24061 ctgcgcttcc agcatacgac cgcaaatcgc catcccgaag cttgggtgat cgacatacgc
    24121 gtcgccagta accaaaatga tgtcgcagct atcccagcca agttgatcca tctcttcacg
    24181 agacatcggc aaaaacggtg ccggtccaaa acaggcggcc cagtactgcg gccaggagaa
    24241 caggtcgcga tccggttgga tcagggagat agagctcatt ttgcttccag aaatgataaa
    24301 aaaataatca aaggccgggg attataagcc ggaacgaaag agaaatcgaa aggtattcca
    24361 tactcgccct cctcgggcga gtatgaagat tacggtaccg gattgaccaa cagttgccct
    24421 atcgagccac ggtccgccat ttccagcgtc tgactgttga agtagaacgg gaagtgcgcc
    24481 caggaaggct gaccgaaata aaccagcagc tccacttgtc cgtcaaccca aacggtatct
    24541 ttccagcctc tgtcttccgg gaacggcatc gcgccattca cgttacggat ctggaacatc
    24601 acgccttcaa tatggaacgc ttgcggctcg tccgcgcgta ccgtccagcg ttcccacgtt
    24661 ccttgctgcg cggtgacatc aatacggttg acgtcccaca gttgtccatt aatacccggg
    24721 tcatcaccca gactgatatc gcgactgcga atcggcgaac cggccatgat ttcagttggc
    24781 agcaggcgca tcggaagact gtctgtgacc agcggcagaa ggccggttgg gcgcagcgtt
    24841 agcaccaggg tagaaaccag aatgctggat ggctcaaaga agccgcgaat acgatcaaca
    24901 atgctcgccg cttcaccaca ggtgatcgac acttcatcac cgttgctcat atccaccaga
    24961 atctcgcggc gctcgcccgg tgccagcgaa agttgcttca ccgatacagg agcgggcagg
    25021 aatccctgat cgccagaaat cacatgtaac gggcgaccat cgctcatctg tagttgatag
    25081 cgacgagagt tcgacgcgtt caacagacgc aagcgcaccc agccacgcga gacttcaacg
    25141 tacgggcttt gtacaccgtt aaccagcagc gtatcaccaa caaagccgcc gcttcccggt
    25201 tcgttatatt ccggcgtacc aaagttatcc agccgtttat cctgaataat gaccggaaaa
    25261 tcatccacac cataatggtt ggggataggc agcgatttgc tgacttcatc ttccaccagc
    25321 cacattccgg caaggccgtt atagacctgc tgagccgtgc ggttgggagt attggcgtga
    25381 taccacagag tagctgcgtt ctggcgaatg ggcagtacgg gtgcccagtc agcgtttggc
    25441 gacatcatcc gtgccggacc gcccatcagc gggccaggta cctgtagccc ggccaccgtc
    25501 attgagacat tttctgtcag gcgattgctg taaataagct taacatcgtc gcccttccag
    25561 acgcggatag tcggccccag gtaacgacca ttgattcccc aaaccgatgc gcgtgtccct
    25621 ggcgtaaatg accagtgcgc acgttgtaca gtcataaaca gcggttgccc acggcgagat
    25681 tcaagcagcg tcggaacggg tagcggttgc tgttgcccgg ctgcgctggc cttcagggga
    25741 acagcgcctg cacaaagtgc aatccccgat gcctgaatga actgacgccg actgagtgac
    25801 atatttgctc catgtaaaac tggctaaagt aataccgtgc aggaaagtgt tccccgcaaa
    25861 aatacagatt gccgacttaa acttttccgg cggcttcgcg ctctgcgact tctttatcga
    25921 gctcagcgat tttttgttcc attatcgaac gacaatgggc agccaactcg cgaacctgat
    25981 ctttgccata ctgactgacg tcaattggcg gcagcatttc gacaatcacc agaccgttgt
    26041 gtagccgatt aagattaatc ttattcgaag ttgtagagac gcacacggga ataatcggga
    26101 cgcccgccgc aattgccgcg tgaaatgccc cggtcttgaa cggcagcagg ccgcgaccac
    26161 ggctgcgggt tccttccggg aacatccaga tggaaatacg gcgttttttg aagtgattca
    26221 cgacttccgc aatagtgccg tgagctttag tgcgattgtt tctgtcgatc aataagttgc
    26281 cggttaacca gtacaactgc ccgaagaagg ggatccacag caagctcttt ttccctaccg
    26341 tcactgtcgg cggctgcaca atgttcgatg ccgtcaccat gtcataattg ttctggtggt
    26401 tagcaatata gatagcattt ccgtagcttt cagcatccgc aggtttgcgg cactcaactt
    26461 tcaggccgaa cagcggtgca agacggccaa acatatgccc aaaggtggcc acatgtttcg
    26521 ggttacgcgg gctgaaaagg cagtaaatgg agccgaatac acagactaag atgctgtaaa
    26581 tcacggtaat aataagacga aagatatata gcataacacc ctctgaaggt ctgagagact
    26641 ggcattgtac gtcacctgaa atcaggttag ggcattgtta aaagccgctc tctggaaaag
    26701 cgtattgtta atcgcaacgc atgaataaac aacggtttca agggaatttt tatcgtttcc
    26761 gggggttgaa tacgctgccg gatgacgact taacgtttca tccggcgttc cttgcaagcg
    26821 ggaggaaaca gcgccctccc cggcatatta ctcttcgcta tcaccgctgc tggcacggcg
    26881 aggagagtcg atctcaacac gatcgatacg ctgcaaaccg cgcatcaacg taccgcggcg
    26941 tccacgttcg ccagcgactt tctgtagctc ttccggacgc agtttaattt tgcgtttccc
    27001 aacatgaatg gtcagcgtgc tttgcggcgg cagaacgtac agttgcgcaa gaccatcctc
    27061 gccacgcgcg gcttctgccg atggaatgtt gataatcttg ttgcctttgc ccttcgacag
    27121 ctgcggcaga tcgcttaccg ggaacatcaa catacggcct gcctgagtga ttgccagcag
    27181 catatcggaa gcatcttcaa tcaccaccgg cggcataaca tgggcatttt ccggtaaggt
    27241 gatcaaagcc ttacctgcac ggttacgcgc caccagatcg ttaaaggtgc agacgaaacc
    27301 gtaacccgca tcggaagcca tcagcagttt ctgatcgtcg ctttccatca gcatatggtc
    27361 aacggtcgct ccaggcggca acgttaattt gccggtgagc ggctcaccct gaccacgcgc
    27421 cgacggcagc gtaatcgggt cgatggcata gctacgaccg gtggaatcaa caaacactac
    27481 cggttggttg ctcttacctt tcaccgccgc tttgaagcta tcgcccgctt tataattcag
    27541 gcccggcgcg tcaatatcat ggcctttagc gctgcgcacc cagcccatct gcgacagcac
    27601 aatggtgaca ggttcagacg gcagcatgtc gtgctcgctc attgctttcg cttcttcgcg
    27661 ttcctgcaac ggcgaacgac gttcgtcacc gtaggcttgc gcgtctgcct gcagttcttt
    27721 cttcagcagg ttattcattt tacgctcgga agccaaaatg ccctgcaact ggtcgcgctc
    27781 tttttccagc tcactctgct caccgcgaat cttcatctct tccagtttgg caagatgacg
    27841 cagtttcagt tcgaggatcg cttccgcctg ggtttcagta aggccaaacc gcgacatcag
    27901 cgccggtttc ggttcatctt cattacgaat gatctcaatc acttcgtcga tattgagaaa
    27961 cgccaccagc aaaccttcga ggatatgcag gcgcttgagg actttctcca gacgatagtt
    28021 cagtcggcgg cgcacggtat cgcggcggaa caccagccat tcggagagga tttccagcag
    28081 gtttttcacc gccggacgac catccagacc gatcatattg aggttaatgc gatagctctt
    28141 ttccagatcg gtggtagcaa agaggtggtt catcacctga tccatatcca cgcggttgga
    28201 acgcggcaca atcaccagac gggtcgggtt ctcgtggtca gattcatcgc gcagatcgtc
    28261 aaccatcggc agctttttgt tgcgcatttg cgcagcaatt tgctccagta cgcgcgcacc
    28321 tgaaacctga tgcggcaatg cgctgataac caccgcgcca tcttctttct tccaaaccgc
    28381 gcgcatacgc actgaaccac gtccgttctc gtagatttta cgaatctcgg cgcgcgaagt
    28441 gataatttcc gcttcagtcg gataatccgg cccctgcacg atatccagca gctgatcgag
    28501 cgtggttttc ggctggtcga ttaatgcgat tgccgcctga gccacttcac gcaggttatg
    28561 cggtggaata tcggtcgcca tgccgacggc aataccggtg gtgccgttaa gcaaaatgtt
    28621 tggcagacgg gcaggcagca ttttcggctc ctgcaaagtg ccgtcgaagt ttggcaccca
    28681 gtcagccgtc ccctgcccca gctcgctcaa tagcagctcg gaatatttcg acaaccggga
    28741 ttcggtgtaa cgcattgccg cgaacgattt cggatcgtcc ggcgcgcccc agttcccctg
    28801 accatcaacc agcggataac ggtaagagaa cggctgcgcc atcaggacca tcgcttcata
    28861 acaggcacta tcgccgtgcg gatggtattt acccagtacg tcaccgacgg tacgggccga
    28921 ttttttaaat ttggcgctgg cattcaggcc cagctcagac atcgcataca caatgcggcg
    28981 ctgaacaggt ttcagaccat caccaataaa cggcaacgca cggtccatga tcacgtacat
    29041 ggagtagttt aagtaggcgt tttccgtaaa ttcatgtagc gcaaggcgct ctgccatatc
    29101 gctcattaat tctgattcct caacttattc gccagccttc aaaggggcaa tattgccgga
    29161 gatactacat tatctgccgc gtcgagtcac aggagatcag cgtgaatagc gccgccggac
    29221 gtgctccggc ggcgataaag gcatcaatgt gccttgatat acaactcttt gctgtagaca
    29281 taatcctggg gattatgcag cggaaaaccg ccaacgtagg gtttaagcag tttgattaac
    29341 ggctgatagt agatgggaat cagcggtgcc tgctggttga tgatcacttc tgcctgctga
    29401 tacaacgcat tacgctttgt cgcatcagtg atctgcgtgg cctggtttag taaggcgtca
    29461 tactgcgcat ttttccagtg accgacgttt tcttcactat cgcttttgag cgtgttcagg
    29521 aagctggagg catcattgta cgtcgcatcc cacgactgcc gagacagcat aaaatcaccg
    29581 gctcgtcggg catcaagata ggttttccac tccattgtgc gcagcgtcac ctgtgcaccc
    29641 agccattttt tccattcgga agacaacgct atcgcggtct tttcatgcag atcgtacttg
    29701 ttgtagaaca gctcaaagcg tagcggatga gaggcgtcgt atcccgcctg tttcagcaag
    29761 gcttttgcca tcgcgacacg ctcactcatt ggcttttgca gttcatcgaa cgtcgtcgcg
    29821 ctaaagcctt ttacctctgg cggcgtcagc gtggttgcgg gcgttctcaa ccccagtacc
    29881 ttttgcgcaa taagctgtcg atcaaccgta agatatagcg cccgacgcac ccgcacatcg
    29941 ttaaatggcg gtttctcaag gttaaagttg taatattcgc tgttcagacg cgggataatt
    30001 cgtagctcgc caggcagtga tttttcaatg gcgggaattt gctgcgccgg aacccaggtg
    30061 agatcgacct ctcccgcgcg atagcggtta tagccggtga ccgaattatc cagcgccaga
    30121 tactcaacct gttgcaatac cgtatgttgc gcatcgcggt actttggatt tttgcgtgcg
    30181 gtaatctttt cgttaactac ccatttatca agcacaaagg caccgttgta aaccatgttc
    30241 tctggcttac tccagctatc gccatgttta gcgatgacat gatgaggaac cgggaacaac
    30301 gtcggccagg cgagcatcgt cgtaaaccaa ggaactggct gctcaagcgt aacttcaaga
    30361 gtacgatcat ccgtcgcttt gatccccagc gatgtaacat ctgctttacc cgcaacaata
    30421 gccgcggcat tgttaatgtg cgcctgtgcc agatagccag caaaagggct tgccgttttc
    30481 gggtcaaccg cacgttgcca gccgaggaca aaatcctctg ccgtcagagg ctgaccgtct
    30541 gaccactgca aaccgctacg cagatggaaa atatagcgct tgccgccgtc aagtatctcc
    30601 cagcgttcag cctgagcggg ctgcacctgg ccttcaccgt ccatccatac cagaccttca
    30661 aacagatcca gcacaatctg cgcggcagta ttctcctcta ccttttgcgg gtcgagcgtg
    30721 cctgggtcgc tatgattgtt gtaacgaaag acttgttgcg gggcgagcgg tgtgttggcg
    30781 ggaacgtcag cagcgtagag aggagccgca cttaccaggc tgaccagcca taataaattt
    30841 cgcgtataca tggtattcct taccggagaa attataagcc cgacaacaat acgcctgtgg
    30901 cattgtttgc tcaaacttat cgcgagtgat ttggctcaca ctccggtaaa gattccccat
    30961 cacgccaacg gcacataaat atccgtttcc agtcgccgct ctgtcatccc ttcggcaaga
    31021 ttggtgtagt gaaacaggat cggggctttg cgcatttttt cgccgcttgc aggtagccag
    31081 tggcgaatga tgccccatat cgtgtgtgaa atatcgtcca gctcgccaac atggcgggca
    31141 acagcataac gcccaccggt aagttcgcca ttactcacgc cataacgatt atcggggatc
    31201 ggttcgctaa cgctgccgca gatatcaaag cgaaacgctt ctggtgcggt ggtggctggg
    31261 tcgtcccagg cgacgccaaa agtctggctt tggttaacag gagaaagccc cgtctcttta
    31321 cgccacatga taaatttcgc cacgctgtag ttgagcaact cagggctaca gcggtgcggc
    31381 aacatcgcca cagggattga tgggaaatcg ataatattta catccagtgt caggtttgtc
    31441 attttgcctc cctgcgaaat tattcccggg aacgcgtccc gggagcggta acaattacgg
    31501 atttactttg cggatctgtt tgacgtcaat ttcaacagag ttccagtctt tatcgacttc
    31561 cccctgaatc tcaaccgtat ctttcggcgt caccgtcacg ccgttccagc gtttatggtc
    31621 gatatcaaca ttgatagtac cgctggcatc tttgaacacg tagagatcgt cagagatgcg
    31681 ttcaacgata ttgccgcgta gggttaccca ggtgtcgtca cgcagggatt ttgcgctttc
    31741 tacagtcgtt acgctgccgt tcggcccctg gaatcctccg gcctgacttt gcgttgccga
    31801 tgggccagaa aaacctccct gctctgctgc cataaccggt gcactgcaca gggccattac
    31861 tgcgattact gctgcgaatt ttttcatgtt tattactccc tttaatgtct gtttccgagc
    31921 atttaacaag atagtcctta acaacttctt aagggaaaaa aataaaattt aatgctgtac
    31981 agagcgcgtt acaacacggt ttactggcag caaatacggt tatcgcaggg atgaaaaaat
    32041 gcgaatttta ctgatagaag atgacatgct gattggcgac ggcatcaaaa cgggccttag
    32101 taaaatgggt tttagcgtcg actggtttac acaaggtcgt cagggaaaag aggcgctata
    32161 tagcgcacct tatgatgcgg tgatcctgga tttaacctta ccgggcatgg atggtcgcga
    32221 tattttgcgc gaatggcgag aaaaaggtca gcgtgagccg gtattgatcc tgactgcgcg
    32281 cgatgcgctg gcggaacgtg tagaagggct gcgtctggga gctgacgatt atctgtgtaa
    32341 accttttgcg ttgatagaag tcgccgccag gctggaagct ctgatgcgcc gaaccaacgg
    32401 ccaggccagc aacgagctgc gccacggcaa cgtcatgcta gaccccggca aacgtatcgc
    32461 cacgctggct ggcgacccct taacgctgaa accaaaagaa tttgccctgc tggaattact
    32521 gatgcgtaac gctggtcggg tactgccgcg caaactgatt gaagagaaac tgtatacctg
    32581 ggacgaagag gtcaccagta atgccgttga agtgcatgtg catcatctgc gacgcaaact
    32641 cggtagtgat tttattcgta ccgtgcatgg tattggctac acattaggtg agaaatgaaa
    32701 tttacccaac gtcttagtct gcgagtcagg ctgacgctaa tctttttaat tctggcctcg
    32761 gtgacctggc tgctttccag ctttgtcgcc tggaaacaaa caacggataa cgtcgatgag
    32821 ttgttcgaca cccaactgat gctgtttgcc aaacggttaa gtacgctcga tctcaacgaa
    32881 atcaacgcgg cggatcgcat ggcacagacg ccaaataaat taaaacacgg tcatgttgat
    32941 gacgatgcgc tgacttttgc catctttacc cacgacggca gaatggtcct taacgatggc
    33001 gataacggag aagatattcc ttatagctat cagcgggaag gttttgctga cgggcaactg
    33061 gtcggtgaag acgatccctg gcgttttgtc tggatgacct cacctgatgg caaatatcgc
    33121 atcgttgtgg gtcaggaatg ggaataccgt gaagacatgg cgctggcgat tgttgccgga
    33181 caattgatcc cgtggctggt cgcactgcca attatgttaa tcatcatgat ggtactactg
    33241 ggtcgtgaac tcgcgccgct gaacaaactg gcgctggcac tacgtatgcg tgaccctgac
    33301 tcggaaaaac cactaaacgc gacgggcgta cccagcgaag tgcgcccact ggttgagtcg
    33361 ctaaatcaac tgttcgcccg cacacatacg atgatggttc gtgaacgacg ctttacctcc
    33421 gacgcagctc acgaacttcg tagcccgtta acggcgctga aagtgcaaac cgaagttgct
    33481 cagctctctg acgatgatcc gcaggcgcgg aaaaaagcgc tgcttcaatt acattccgga
    33541 atcgatcgtg ctactcgtct ggttgatcaa ctgctcacgc tatcgcggct ggactcactg
    33601 gataaccttc aggacgtcgc ggagatcccg cttgaagatc tcctgcaatc gtcggtgatg
    33661 gatatttacc acacggcgca gcaggcgaac attgacgtgc gactgacact caatgccaac
    33721 ggcatcaaac gcaccggaca accgctattg ctaagtttgt tggtgcgaaa tttgctggat
    33781 aacgccgtgc gctacagtcc acagggcagc gtggtggacg tcacgctgaa tgccgataat
    33841 ttcatcgtga gggataacgg ccccggtgtg acaccagagg cactggcgag aattggcgaa
    33901 cgcttctatc gcccacccgg acaaaccgct accggcagtg gacttgggct atcaattgtc
    33961 cagcgaatcg ccaagctgca tgacatgaat gttgaatttg ggaatgcgga acaaggtgga
    34021 tttgaggcga aagtaagctg gtaacttctc tgtctatgag agccgttaaa acgactctca
    34081 tagattttat taatagcaaa atataaaccg tccccaaaaa agccaccaac cacaaaccaa
    34141 acaaataacg agcgagatta catataacca
//
LOCUS       DAZFLD010000019        33977 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.019, whole genome shotgun sequence.
ACCESSION   DAZFLD010000019 DAZFLD010000000
VERSION     DAZFLD010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33977
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.019"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..1422)
                     /locus_tag="ACHKBL_000855"
     CDS             complement(<1..1422)
                     /locus_tag="ACHKBL_000855"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HFO5817768.1"
                     /translation="MEQKITDYLDEIYGGTFTATHLQKLVTRLESAKRLITQRRKKHW
                     DESDVVLITYADQFHSNDLKPLPTFNQFYHQWLQSTFSHVHLLPFYPWSSDDGFSVID
                     YHQVASEAGEWQDIQQLGECSHLMFDFVCNHMSAKSEWFKNYLQQQPGFEDFFIAVDP
                     QTDLSAVTRPRALPLLTPFQMRDNSTRHLWTTFSDDQIDLNYRSPEVLLAMVDVLLCY
                     LEKGAEYIRLDAVGFMWKEPGTSCIHLEKTHLIIKLLRSIIDNVAPGTVIITETNVPH
                     IDNIAYFGNGDDEAHMVYQFSLPPLVLHAVQKQNVEALCAWAQNLTLPSSNTTWFNFL
                     ASHDGIGLNPLRGLLPENEILALVEVLQQEGALVNWKNNPDGTRSPYEMNVTYMDALS
                     RRECSDEERCARFILAHAILLSFPGVPAIYIQSILGSRNDYAGVEKLGYNRAINRKKY
                     SSKEITTELNNKATLRYLIYYKLS"
     gene            complement(1636..1950)
                     /gene="pspE"
                     /locus_tag="ACHKBL_000856"
     CDS             complement(1636..1950)
                     /gene="pspE"
                     /locus_tag="ACHKBL_000856"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HFO5817769.1"
                     /translation="MFKKGLLALTLVFSLPVFAAEHWIDVRVPEQYQQEHVQGAINIP
                     LKEVKERIATAVPDKNDTVKVYCNAGRQSGQAKEILSEMGYTHVENAGGLKDIAMPKV
                     KG"
     gene            complement(2025..2246)
                     /gene="pspD"
                     /locus_tag="ACHKBL_000857"
     CDS             complement(2025..2246)
                     /gene="pspD"
                     /locus_tag="ACHKBL_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspD"
                     /protein_id="HFO5817770.1"
                     /translation="MNTRWQQAGQKVKPGFKLAGKLVLLTALRYGPAGVAGWAIKSVA
                     RRPLKMLLAVALEPLLSRAANKLAQRYKR"
     gene            complement(2255..2614)
                     /gene="pspC"
                     /locus_tag="ACHKBL_000858"
     CDS             complement(2255..2614)
                     /gene="pspC"
                     /locus_tag="ACHKBL_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HFO5817771.1"
                     /translation="MAGINLNKKLWRIPQQGMVRGVCAGIANYFDVPVKLVRILVVLS
                     IFFGLALFTLVAYIILSFALDPMPDNMAFGEQLPSSSELLDEVDRELAASETRLREME
                     RYVTSDTFTLRSRFRQL"
     gene            complement(2614..2838)
                     /gene="pspB"
                     /locus_tag="ACHKBL_000859"
     CDS             complement(2614..2838)
                     /gene="pspB"
                     /locus_tag="ACHKBL_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009271 - phage shock [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HFO5817772.1"
                     /translation="MSALFLAIPLTIFVLFVLPIWLWLHYSNRSGRSELSQSEQQRLA
                     QLADEAKRMRERIQALESILDAEHPNWRDR"
     gene            complement(2892..3560)
                     /gene="pspA"
                     /locus_tag="ACHKBL_000860"
     CDS             complement(2892..3560)
                     /gene="pspA"
                     /locus_tag="ACHKBL_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HFO5817773.1"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVR
                     STSARALAEKKQLTRRIEQASAREVEWQEKAELALLKEREDLARAALIEKQKLTDLIK
                     SLEHEVTLVDDTLARMKKEIGELENKLSETRARQQALMLRHQAANSSRDVRRQLDSGK
                     LDEAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMK
                     QDNQ"
     gene            3727..4704
                     /gene="pspF"
                     /locus_tag="ACHKBL_000861"
     CDS             3727..4704
                     /gene="pspF"
                     /locus_tag="ACHKBL_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HFO5817774.1"
                     /translation="MAEYKDNLLGEANSFLEVLEQVSHLAPLDKPVLIIGERGTGKEL
                     IASRLHYLSSRWQGPFISLNCAALNENLLDSELFGHEAGAFTGAQKRHPGRFERADGG
                     TLFLDELATAPMMVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPAMVNEGT
                     FRADLLDRLAFDVVQLPPLRERESDIMLMAEHFAIQMCREIKLPLFPGFTERARETLL
                     NYRWPGNIRELKNVVERSVYRHGTSDYPLDDIIIDPFKRRPPEDAIAVSETTSLPTLP
                     LDLREFQMQQEKELLQLSLQQGKYNQKRAAELLGLTYHQFRALLKKHQI"
     gene            complement(4824..6089)
                     /gene="puuE"
                     /locus_tag="ACHKBL_000862"
     CDS             complement(4824..6089)
                     /gene="puuE"
                     /locus_tag="ACHKBL_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009448 - gamma-aminobutyric acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate transaminase"
                     /protein_id="HFO5817775.1"
                     /translation="MSNNEFHQRRLSATPRGVGVMCNFFAQSAENATLKDVEGNEYID
                     FAAGIAVLNTGHRHPDLVAAVEQQLQQFTHTAYQIVPYESYVTLAEKINALAPVSGQA
                     KTAFFTTGAEAVENAVKIARAHTGRPGVIAFSGGFHGRTYMTMALTGKVAPYKIGFGP
                     FPGSVYHVPYPSDLHGISTQDSLDAIERLFKSDIEAKQVAAIIFEPVQGEGGFNVAPK
                     ELVAAIRRLCDEHGIVMIADEVQSGFARTGKMFAMDHYADKPDLMTMAKSLAGGMPLS
                     GVVGNANIMDAPAPGGLGGTYAGNPLAVAAAHAVLNIIDKESLCERANQLGQRLTNTL
                     IDAKESVPAIAAVRGLGSMIAAEFNDPQTGEPSAAIAQKIQQRALAQGLLLLTCGAYG
                     NVIRFLYPLTIPDAQFDEAMKILQDALSD"
     gene            complement(6127..7407)
                     /gene="puuB"
                     /locus_tag="ACHKBL_000863"
     CDS             complement(6127..7407)
                     /gene="puuB"
                     /locus_tag="ACHKBL_000863"
                     /EC_number="1.4.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylputrescine oxidase"
                     /protein_id="HFO5817776.1"
                     /translation="MTEHTSSYYAASANKYAPFDTLNESISCDVCVVGGGYTGLSSAL
                     HLAEAGFDVVVLEASRIGFGASGRNGGQLVNSYSRDIDVIEKSYGMDTARMLGSMMFE
                     GGEIIRERIKRYQIDCDYRPGGLFVAMNDKQLATLEEQKENWERYGNKQLELLDANAI
                     RREVASDRYTGALLDHSGGHIHPLNLAIGEADAIRLNGGRVHELSAVTQIQHTTPAVV
                     RTANGQVTAKYVIVAGNAYLGDKVEPELAKRSMPCGTQVITTERLSEDLARSLIPKNY
                     CVEDCNYLLDYYRLTADNRLLYGGGVVYGARDPDDVERLVVPKLLKTFPQLKGVKIDY
                     RWTGNFLLTLSRMPQFGRLDTNIYYMQGYSGHGVTCTHLAGRLIAELLRGDAERFDAF
                     ANLPHYPFPGGRTLRVPFTAMGAAYYSLRDRLGV"
     gene            complement(7409..8896)
                     /gene="puuC"
                     /locus_tag="ACHKBL_000864"
     CDS             complement(7409..8896)
                     /gene="puuC"
                     /locus_tag="ACHKBL_000864"
                     /EC_number="1.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase PuuC"
                     /protein_id="HFO5817777.1"
                     /translation="MNFHHLAYWQDKALSLAIETRLFINGEYTAAAENETFETVDPVT
                     QAPLANIARGKSVDIDRAVSAARGVFERGDWSLSSPAKRKAVLNKLADLMEANAEELA
                     LLETLDTGKPIRHSLRDDIPGAARAIRWYAEAIDKVYGEVATTSSHELAMIVREPVGV
                     IAAIVPWNFPLLLTCWKLGPALAAGNSVILKPSEKSPLSAIRLAGLAKEAGLPDGVLN
                     VVTGFGHEAGQALSRHNDIDAIAFTGSTRTGKQLLKDAGDSNMKRVWLEAGGKSANIV
                     FADCPDLQKAASATAAGIFYNQGQVCIAGTRLLLEESIADEFLALLKQQAQNWQPGHP
                     LDPATTMGTLIDCAHADSVHSFIREGESKGQLLLDGRNAELAAAIGPTIFVEVDPNAS
                     LSREEIFGPVLVVTRFTSEEQALQLANDSQYGLGAAVWTRDLSRAHRMSRRLKAGSVF
                     VNNYNDGDMTVPFGGYKQSGNGRDKSLHALEKFTELKTIWISLEA"
     gene            complement(9046..9603)
                     /gene="puuR"
                     /locus_tag="ACHKBL_000865"
     CDS             complement(9046..9603)
                     /gene="puuR"
                     /locus_tag="ACHKBL_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator PuuR"
                     /protein_id="HFO5817778.1"
                     /translation="MSDEGLAPGKRLSEIRQQQGLSQRRAAELSGLTHSAISTIEQDK
                     VSPAISTLQKLLKVYGLSLSEFFSEPEKPDEPQVVINQDDLIEMGSQGVSMKLVHNGN
                     PNRTLAMIFETYQPGTTTGERIKHQGEEIGTVLEGEIVLTINGQDYHLVAGQSYAINT
                     GIPHSFSNTSAGICRIISAHTPTTF"
     gene            complement(9630..10394)
                     /gene="puuD"
                     /locus_tag="ACHKBL_000866"
     CDS             complement(9630..10394)
                     /gene="puuD"
                     /locus_tag="ACHKBL_000866"
                     /EC_number="3.5.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase"
                     /protein_id="HFO5817779.1"
                     /translation="MENIMNNPVIGVVMCRNRLKGHATQTLQEKYLNAIIHAGGLPIA
                     LPHALAEPSLLEQLLPKLDGIYLPGSPSNVQPHLYGENGDEPDADPGRDLLSMALINA
                     ALERRIPIFAICRGLQELVVATGGSLHRKLCEQPELLEHREDPELPVEQQYAPSHEVQ
                     VEEGGLLSALLPECSNFWVNSLHGQGAKVVSPRLRVEARSPDGLVEAVSVINHPFALG
                     VQWHPEWNSSEYALSRILFEGFITACQHHIAEKQRL"
     gene            10606..12024
                     /gene="puuA"
                     /locus_tag="ACHKBL_000867"
     CDS             10606..12024
                     /gene="puuA"
                     /locus_tag="ACHKBL_000867"
                     /EC_number="6.3.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415813.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-putrescine ligase"
                     /protein_id="HFO5817780.1"
                     /translation="METNIVEVENFVQQSEERRGSSFTQEVKRYLERYPNTQYVDVLL
                     TDLNGCFRGKRIPVSSLKKLEKGCYFPASVFAMDILGNVVEEAGLGQEMGEPDRTCVP
                     VLGSLTPSAADPEFIGQMLLTMVDEDGAPFDVEPRNVLNRLWQQLRQRGLFPVVAVEL
                     EFYLLDRQRDAEGYLQPPCAPGTDDRNTQSQVYSVDNLNHFADVLNDIDELAQLQLIP
                     ADGAVAEASPGQFEINLYHTDNVLEACDDALALKRLVRLMAEKHKMHATFMAKPYEEH
                     AGSGMHIHISMQNNRGENVLSDAEGEDSPLLKKMLAGMIDLMPSSMALLAPNVNSYRR
                     FQPGMYVPTQASWGHNNRTVALRIPCGDRHNHRVEYRVAGADANPYLVMAAIFAGILH
                     GLDNELPLQEEVEGNGLEQEGLPFPIRQSDALGEFIENDHLRRYLGERFCHVYHACKN
                     DELLQFERLITETEIEWMLKNA"
     gene            12173..12337
                     /locus_tag="ACHKBL_000868"
     CDS             12173..12337
                     /locus_tag="ACHKBL_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5817781.1"
                     /translation="MPMIKSPHGEGGCVCAPPATDWTPPPLLPLLNRFDFRSTRPQTL
                     LRRGGSNYGY"
     gene            12327..13712
                     /gene="puuP"
                     /locus_tag="ACHKBL_000869"
     CDS             12327..13712
                     /gene="puuP"
                     /locus_tag="ACHKBL_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415812.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PuuP"
                     /protein_id="HFO5817782.1"
                     /translation="MAINSPLNIAAQPGKTRLRKSLKLWQVVMMGLAYLTPMTVFDTF
                     GIVSGISDGHVPASYLLALAGVLFTAISYGKLVRQFPEAGSAYTYAQKSINPHVGFMV
                     GWSSLLDYLFLPMINVLLAKIYLSALFPEVPPWVWVVTFVAILTAANLKSVNLVANFN
                     TLFVLVQISIMVVFIFLVVQGLHKGEGVGTVWSLQPFISENAHLIPIITGATIVCFSF
                     LGFDAVTTLSEETPDAARVIPKAIFLTAVYGGVIFIAASFFMQLFFPDISRFKDPDAA
                     LPEIALYVGGKLFQSIFLCTTFVNTLASGLASHASVSRLLYVMGRDNVFPERVFGYVH
                     PKWRTPALNVIMVGIVALSALFFDLVTATALINFGALVAFTFVNLSVFNHFWRRKGMN
                     KSWKDHFHYLLMPLVGALTVGVLWVNLESTSLTLGLVWASLGGAYLWYLIRRYRKVPL
                     YDGDRTPVSET"
     gene            13846..14091
                     /gene="ymjA"
                     /locus_tag="ACHKBL_000870"
     CDS             13846..14091
                     /gene="ymjA"
                     /locus_tag="ACHKBL_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmjA family protein"
                     /protein_id="HFO5817783.1"
                     /translation="MNHDIPLKYFDIADEYATECAEPVADAERTPLALYFQLLLTRLM
                     NNEEISEEAQHEMAAEAGINPVRIDEIAEFLNQWGNE"
     gene            14404..16047
                     /gene="sapA"
                     /locus_tag="ACHKBL_000871"
     CDS             14404..16047
                     /gene="sapA"
                     /locus_tag="ACHKBL_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein SapA"
                     /protein_id="HFO5817784.1"
                     /translation="MRQVLSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQV
                     NTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLMPELAESWEVLDNGATYRFHLRR
                     DVPFQKTDWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSV
                     RKLDNHTVEFRLAQPDASFLWHLATHYASVMSAEYARKLEKEDRQEQLDRQPVGTGPY
                     QLSEYRAGQYIRLQRHDDFWRGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAAS
                     QLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALALAINNQRLMQSIYYG
                     TAETAASILPRASWAYDNEAKITEYNPAKSREQLKALGLENLTLKLWVPTRSQAWNPS
                     PLKTAELIQADMAQVGVKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRP
                     LLSCAAIHSQTNLAHWCDPKFDSVLRKALSSQQLAARIEAYDEAQSILAQELPILPLA
                     SSLRLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP"
     gene            16044..17009
                     /gene="sapB"
                     /locus_tag="ACHKBL_000872"
     CDS             16044..17009
                     /gene="sapB"
                     /locus_tag="ACHKBL_000872"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine export ABC transporter permease SapB"
                     /protein_id="HFO5817785.1"
                     /translation="MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAW
                     VFWFNGLIHWDFGVSSINGQPIAEQLKEVFPATMELCILAFGFALIVGIPVGMIAGIT
                     RHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFA
                     LIDAWLSDSPWRDEMVMSAIRHMILPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAA
                     ATRGLSRFTILRRHVLHNALPPVIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAI
                     RQQDYAAISAGVMVCGSLVIIVNVISDILGAMANPLKHKEWYALR"
     gene            16996..17886
                     /gene="sapC"
                     /locus_tag="ACHKBL_000873"
     CDS             16996..17886
                     /gene="sapC"
                     /locus_tag="ACHKBL_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapC"
                     /protein_id="HFO5817786.1"
                     /translation="MPYDSVYSEKRPPGTLRTAWRKFYSDASAMVGLYGCAGLAVLCI
                     FGGWFAPYGIDQQFLGYQLLPPSWSRYGEVSFFLGTDDLGRDVLSRLLSGAAPTVGGA
                     FVVTLAATICGLVLGTFAGATHGLRSAVLNHILDTLLAIPSLLLAIIVVAFAGPSLSH
                     AMFAVWLALLPRMVRSIYSMVHDELEKEYVIAARLDGASTLNILWFAVMPNITAGLVT
                     EITRALSMAILDIAALGFLDLGAQLPSPEWGAMLGDALELIYVAPWTVMLPGAAIMIS
                     VLLVNLLGDGVRRAIIAGVE"
     gene            17886..18878
                     /gene="sapD"
                     /locus_tag="ACHKBL_000874"
     CDS             17886..18878
                     /gene="sapD"
                     /locus_tag="ACHKBL_000874"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapD"
                     /protein_id="HFO5817787.1"
                     /translation="MPLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGS
                     GKSLIAKAICGVNKDNWRVTADRMRFDDIDLLRLSARERRKLVGHNVSMIFQEPQSCL
                     DPSERVGRQLMQNIPAWTYKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELT
                     EGECQKVMIAIALANQPRLLIADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHD
                     LQMLSQWADKINVLYCGQTVETAPSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLN
                     TLPGAIPLLEQLPIGCRLGPRCPYAQRECIVTPRLTGAKNHLYACHFPLNMEKE"
     gene            18880..19686
                     /gene="sapF"
                     /locus_tag="ACHKBL_000875"
     CDS             18880..19686
                     /gene="sapF"
                     /locus_tag="ACHKBL_000875"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapF"
                     /protein_id="HFO5817788.1"
                     /translation="MIETLLEVRNLSKTFRYRTGWFRRQTVEAVKPLSFTLREGQTLA
                     IIGENGSGKSTLAKMLAGMIEPTSGELLIDDHPLHFGDYSFRSQRIRMIFQDPSTSLN
                     PRQRISQILDFPLRLNTDLEPEQRRKQIIETMRMVGLLPDHVSYYPHMLAPGQKQRLG
                     LARALILRPKVIIADEALASLDMSMRSQLINLMLELQEKQGISYIYVTQHIGMMKHIS
                     DQVLVMHQGEVVERGSTADVLASPLHELTKRLIAGHFGEALTADAWRKDR"
     gene            complement(19735..20898)
                     /locus_tag="ACHKBL_000876"
     CDS             complement(19735..20898)
                     /locus_tag="ACHKBL_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HFO5817789.1"
                     /translation="MARVSLSWALILGLLAGIGPMCTDLYLPALPEMSEQLAATTTIT
                     QLTLTASLIGLGVGQLLFGPLSDKIGRKRPLILSLLLFIVSSILCATTNNIYWLVVWR
                     FIQGIAGAGGSVLSRSIARDKYQGVTLTQFFALLMTVNGLAPVLSPVLGGYIVSTFDW
                     RTLFWVMAEISTVLLLGCLLFINETLPENKRGSSLLLTGRSVVQNRRFIRFCLIQSFM
                     LAGLFAYIGSSSFVLQKEFGFSPMQFSLVFGLNGIGLIIASWIFSRLARRINAMTLLR
                     GGLIAAILCALLTVLCAWIQLPIPALVALFFTIAFCSGIGTVGGAEAMSAVGTQESGT
                     ASALMGMSMFVFGGIAAPLSGIGGETLLKMSLAITVCYTLALLVALTRIDNQK"
     gene            complement(20907..22280)
                     /locus_tag="ACHKBL_000877"
     CDS             complement(20907..22280)
                     /locus_tag="ACHKBL_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HFO5817790.1"
                     /translation="MLRRSLIFLVLLSAGCVSLDPHYSTPESPIPATLPGAQGQGKAI
                     SHDWQQVIHDPRLQQVVTIALNSNRDVQKAIADIDSARALYGQTNASLFPTVNAALSS
                     TRSRSLANGTGTTAEADGTVSSYTLDLFGRNQSLSRAAQETWLASEFTAQNTRLTLIA
                     EISTAWLTLAADNSNLALAKETMTSAENSLKIIQRQQQIGTAAATDVSEAMSVYQQAR
                     ASVASYQTQVMQDKNALNLLAGTTLAENLLPGTLESLPEQMISLVPAGVSSDVLLRRP
                     DIQEAEHNLKSANADIGAARANFFPTISLTASAGVGSDALSSLFSHGMQIWSFAPSVT
                     LPLFTGGSNLAQLRYAEAQKRGLIATYEKTVQSAFKDVANALARRTTLEEQLDAQRQY
                     VKAEQQTVDVGLRRYQAGVGDYLTVLTAQRSLWSAQQELLALQLTDFTNRITLWQSLG
                     GGMSSLK"
     gene            complement(22284..25391)
                     /locus_tag="ACHKBL_000878"
     CDS             complement(22284..25391)
                     /locus_tag="ACHKBL_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HFO5817791.1"
                     /translation="MFSRFFVRRPVFAWVIAILIMLAGILAIRTLPVAQYPDVAPPTI
                     KISATYTGASAETLENSVTQVIEQQLTGLDNLLYFSSTSSSDGSVSINVTFEQGTDPD
                     TAQVQVQNKIQQAESRLPSEVQQTGVTVEKSQSNFLLIAAVYDTTDKASSSDIADWLV
                     SNVQDPLARVEGVGSLQVFGAEYAMRIWLDPAKLASYSLMPSDVQSAIEAQNVQVTAG
                     KIGALPSPNTQQLTATVRAQSRLQTVDQFKNIIVKSQSDGAVVRIKDVARVEMGSEDY
                     TAIGKLNGHPSAGVAVMLSPGANALNTATLVKDKIAEFQRNMPQGYDIAYPKDSTEFI
                     KISVEDVIQTLFEAIVLVVCVMYLFLQNLRATLIPALAVPVVLLGTFGVLALFGYSIN
                     TLTLFAMVLAIGLLVDDAIVVVENVERIMRDEGLPAREATEKSMGEISGALVAIALVL
                     SAVFLPMAFFGGSTGVIYRQFSITIISAMLLSVVVALTLTPALCGSVLQHVPPHKKGF
                     FGAFNRFYRRTEDKYQRGVIYVLRRAARTMGLYVVLGGGMALMMWKLPGSFLPTEDQG
                     EIMVQYTLPAGATAARTAEVNRQIVDWFLINEKANTDVIFTVDGFSFSGSGQNTGMAF
                     VSLKNWSQRKGAENTAQAIALRATKELGTIRDATVFAMTPPAVDGLGQSNGFTFELLA
                     NGGTDRETLLQMRNQLIEKANQSPELHSVRANDLPQMPQLQVDIDSNKAVSLGLSLND
                     VTDTLSSAWGGTYVNDFIDRGRVKKVYIQGDSEFRSAPSDLGKWFVRGSDNAMTPFSA
                     FATTRWLYGPERLVRYNGSAAYEIQGENATGFSSGDAMTKMEELANSLPAGTTWAWSG
                     LSLQEKLASGQALSLYAVSILVVFLCLAALYESWSVPFSVILVIPLGLLGAALAAWMR
                     DLNNDVYFQVALLTTIGLSSKNAILIVEFAEAAVAEGYSLSRAALRAAQTRLRPIIMT
                     SLAFIAGVMPLAIATGAGANSRIAIGTGIIGGTLTATLLAIFFVPLFFVLVKRLFAGK
                     PRRQE"
     gene            complement(25391..26512)
                     /locus_tag="ACHKBL_000879"
     CDS             complement(25391..26512)
                     /locus_tag="ACHKBL_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HFO5817792.1"
                     /translation="MKYIATSVVAMLLLSGCDNTQSNNSSPSETEVGVVTLKSQPVSV
                     VSELTGRTSAALSAEVRPQVGGIIQKRLFKEGDLVKAGQPLYQIDAASYQAAWNEARA
                     ALQQAQALVKADCQKAQRYARLVKENGVSQQDADDAQSTCAQDKASVEAKKAALETAR
                     INLDWTTVTAPISGRIGISSVTPGALVTASQDTALTTIRGLDTMYVDLTRSSVDLLRL
                     RKQSLATNSDTMSVSLILEDGTTYSEKGRLELTEVAVDESTGSVTLRAIFPNPQQQLL
                     PGMFVRARVDEGVMEDAILAPQQGVTRDAKGNATALVVNKDNKVEQRTLETGETYGDK
                     WLVLNGLHNGDRLIVEGSAKVTSGQTVKAVEVQANGGNA"
     gene            complement(26661..27227)
                     /locus_tag="ACHKBL_000880"
     CDS             complement(26661..27227)
                     /locus_tag="ACHKBL_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFO5817793.1"
                     /translation="MTSKLEIRHKQRQDEIINAARRCFRRCGFHAASMSQIASEAQLS
                     VGQIYRYFANKDAIIEEMVRRIIDFRIAQMDIDARTDHFPEVLALRKSLNEDDDALML
                     EVAAEATRNPRVMAMLEEADARMFANGCAHMKRMHPHLSDEHIRCCVEVFATMMEGTV
                     YRRLTPQKSDPQHLQEIYQDIVSMLINK"
     gene            27705..28493
                     /gene="fabI"
                     /locus_tag="ACHKBL_000881"
     CDS             27705..28493
                     /gene="fabI"
                     /locus_tag="ACHKBL_000881"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HFO5817794.1"
                     /translation="MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKL
                     KGRVEEFAAQLGSDIVLQCDVAEDTSIDTMFAELGKVWPKFDGFVHSIGFAPGDQLDG
                     DYVNAVTREGFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMG
                     LAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRR
                     TVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK"
     gene            28637..29764
                     /gene="yciW"
                     /locus_tag="ACHKBL_000882"
     CDS             28637..29764
                     /gene="yciW"
                     /locus_tag="ACHKBL_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain-containing protein"
                     /protein_id="HFO5817795.1"
                     /translation="MEKRHITGKSYWYHETQSSTAEYDVLPLVPEAAKVSDPFLLDVI
                     LDEETLAPFLSWLVPARVLAVELFPDQLTVTRSQTFTAYERLSTALTVAQVCGVQRLC
                     NYYSARLTPLPGPDSSRESNHRLAQITQYARQLASSPSIIDNRSRQHLNDVGLTAWDC
                     VIINQIIGFIGFQARSIATFQAYLGHPVRWLPGLEIQNYADASLFADESIRWRSSYEV
                     EKLPEEHTKSSTAELCQLAETLSLHPISLSLLEKLLNSTRVNTQPDNQLAALLCARIN
                     GSPACFATCMNSSNEYKKISALLRKGENEINQWADRHSVERATVQAIQWLTRAPDRFS
                     AAQFSPLLEHEKSSTQIINLLVWSGLCGWINRLKIALGETY"
     gene            29832..31766
                     /gene="rnb"
                     /locus_tag="ACHKBL_000883"
     CDS             29832..31766
                     /gene="rnb"
                     /locus_tag="ACHKBL_000883"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008859 - exoribonuclease II activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HFO5817796.1"
                     /translation="MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAQKSY
                     FIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVEPFLTRFVGKVQGKNDRLAIVPD
                     HPLLKDAIPCRAARGLNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVP
                     WWVTLARHNLEKEAPDGVATEMLDEGLVREDLTALDFVTIDSASTEDMDDALFAKALP
                     DDKLQLIVAIADPTAWIAEGSKLDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRAN
                     EVRPVLACRMTLSADGTIEDNIEFFAATIESKAKLVYDLVSDWLENTGDWKPESEAIA
                     EQVRLLAQICQRRGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRIANRIVEEA
                     MIAANICAARVLRDKLGFGIYNVHMGFDPANADALAALLKTHGLHVDAEEVLTLDGFC
                     KLRRELDAQPTGFLDSRIRRFQSFAEISTEPGPHFGLGLEAYATWTSPIRKYGDMINH
                     RLLKAVIKGETATRPQDEITVQMAERRRLNRMAERDVGDWLYARFLKDKAGTDTRFAA
                     EIVDISRGGMRVRLVDNGAIAFIPAPFLHAVRDELVCSQENGTVQIKGETAYKVTDVI
                     DVTIAEVRMETRSIIARPVA"
     gene            complement(31843..31947)
                     /locus_tag="ACHKBL_000884"
     CDS             complement(31843..31947)
                     /locus_tag="ACHKBL_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5817797.1"
                     /translation="MTYMKLTAAKRIVFFIYLFVIQFWKNASFQTKVS"
     gene            32001..>33977
                     /gene="pdeR"
                     /locus_tag="ACHKBL_000885"
     CDS             32001..>33977
                     /gene="pdeR"
                     /locus_tag="ACHKBL_000885"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HFO5817798.1"
                     /translation="MKTVRESTTLYNFLGSHNPYWRLTESSDVLRFSTTETTEPERTL
                     QLSAEQAARIREMTVITSSLMMSLTVDESDLSVHLVGRKINKREWAGNASAWHDTPAV
                     ARDLSHGLSFAEQVVSEAHSAIVILDSRGNIQRFNRLCEDYTGLKEHDVIGQSVFKLF
                     MSRREAAASRRNNRVFFRSGNAYEVELWIPTRKGQRLFLFRNKFVHSGSGKNEIFLIC
                     SGTDITEERRAQERLRILANTDSITGLPNRNAMQELIDHAINQADNNKVGVVYLDLDN
                     FKKVNDAYGHLFGDQLLRDVSLAILSCLEHDQVLARPGGDEFLVLASNTSQSALEAMA
                     SRILTRLRLPFRIGLIEVYTSCSVGISLSPEHGSDSAAIIRHADTAMYTAKEGGRGQF
                     CVFTPEMNQRVFEYLWLDTNLRKALENDQLVIHYQPKITWRGEVRSLEALVRWQSPER
                     GLIPPLDFISYAEESGLIVPLGRWVILDVVRQVAKWRDKGINLRVAVNISARQLADQT
                     IFTALKQVLQELNFEYCPIDVELTESCLIENDELALSVIQQFSQLGAQVHLDDFGTGY
                     SSLSQLARFPIDAIKLDQVFVRDIHKQPVSQSLVRAIVAVAQALNLQVIAEGVESAKE
                     DAFLTKNGINERQGFLFAKPMPAVAFERWYKRYLK"
BASE COUNT         7996 a         8982 c         8572 g         8427 t
ORIGIN      
        1 cgacaactta taatatatca aataccttaa tgtcgcttta ttgttcagtt ctgtcgttat
       61 ctctttacta gagtattttt tacggttaat cgcacgatta tatccaagtt tttcgacacc
      121 tgcgtaatca ttacgcgacc ccagaatgct ttgaatatat atcgctggca caccggggaa
      181 acttaacaaa atcgcatggg cgagaataaa cctggcacac cgttcttcgt cgctacactc
      241 acggcggctc aacgcatcca tataggtgac attcatttca taggggctac gcgtaccgtc
      301 gggattattt ttccagttta ctaatgcccc ttcctgctgt aacacctcga ccagcgctaa
      361 tatttcgttt tcgggcaaca acccccgcag tgggttaagc ccgatgccat cgtgagaggc
      421 aaggaagtta aaccaggtgg tgttgctgga aggtagtgtc aggttttgcg cccacgcaca
      481 aagtgcctca acgttctgtt tttgcaccgc atgcagcacc agcggcggca gcgagaactg
      541 gtacaccata tgcgcttcgt catcaccgtt gccaaagtaa gcaatgttgt ctatatgcgg
      601 aacattggtc tcggtaatga tcactgtgcc tggcgcaacg ttatcaataa tcgaccgtaa
      661 cagtttgata atcagatgtg ttttttccag atggatgcag cttgttcccg gctctttcca
      721 cataaagcca acggcatcca gacggatata ctcagcacct ttctcaaggt aacacagtaa
      781 aacatccacc atcgccagta acacttcagg gctacggtaa ttgaggtcaa tttgatcgtc
      841 gctaaaggtg gtccataaat gacgcgttga attatcgcgc atctggaatg gcgttaataa
      901 cggtaacgca cgcggacggg tgacggcgct gagatcggtt tgcggatcaa ccgcaataaa
      961 aaaatcttca aaacctggct gctgttgtaa atagttttta aaccattcac tttttgccga
     1021 catatggttg cagacaaaat caaacattaa atgactgcat tcaccgagtt gctgaatatc
     1081 ctgccactcc cccgcttcac tggcgacctg atgataatca attaccgaaa agccatcatc
     1141 agatgaccat ggataaaacg gcaacaaatg aacatgtgaa aaagtgcttt gcagccattg
     1201 atggtaaaac tgattaaatg tgggtagtgg ttttaaatca ttactgtgaa attgatcggc
     1261 ataggtaatt aacacgacat cactttcatc ccagtgtttt ttacgtcgct gtgtaattaa
     1321 tcgtttcgca ctctcaagac gcgttacaag tttctgtaaa tgagtcgcgg taaatgttcc
     1381 accgtagatt tcgtccaggt aatccgtaat tttctgttcc atgttgcctt ttcgtggtag
     1441 cggtcccatg aaacgagaga ctactccgca gaaaataaac tgtcaattga gcacaagggt
     1501 tttagtcacg caggaaatca ttttttacca gggaaaaagc gtatttgtga gctgacgcaa
     1561 gtaatctatg gggatgtaaa taaggtgtga cagaaaaagt aaacggcgca taagcgccgc
     1621 ttagggtgga ttcttttaac ctttgacctt cggcattgcg atgtctttca ggccgccggc
     1681 gttctcaacg tgggtatatc ccatctcgct aaggatctct tttgcctgcc ctgactggcg
     1741 tccggcattg caatacactt tcacggtgtc gtttttatcc ggaacggcgg tggcaatgcg
     1801 ctctttcact tccttcaggg gaatattgat ggctccctga acgtgctctt gctgatactg
     1861 ctctggaaca cgaacatcga tccagtgttc agcggcgaaa acgggcagtg aaaacaccag
     1921 tgtcagagca agtaagcctt ttttaaacat gtaagcgtcc ttctatggat gaaaattgcc
     1981 atccataata cgccttagct ttaacaattt ttgcgaaact cccctcacct tttataacgc
     2041 tgcgccagtt tattggcagc ccgacttaac agcggttcca gcgccacagc cagcaacatt
     2101 ttcagtggtc ggcgagcaac tgattttatc gcccagccag ctacacccgc cgggccatag
     2161 cgcagtgcgg taagaagtac cagcttgcct gctaatttga aaccaggctt caccttttgc
     2221 ccggcctgtt gccagcgagt attcataact ttcctcacag ttgacggaaa cggctacgta
     2281 acgtgaaagt atcggaagtg acataacgtt ccatctcgcg taaacgcgtt tcacttgccg
     2341 ccagttcacg gtcgacttca tccagcaatt cactgctgga aggtagctgc tcaccaaagg
     2401 ccatgttgtc cggcattgga tcaagcgcaa atgacaaaat gatgtaagca accagggtaa
     2461 acagcgccag accgaagaaa atcgacagca ccaccaggat acgcaccagt tttactggta
     2521 catcaaaata gttggcaatc ccggcgcaga cgccacggac catgccctgc tgtggaatac
     2581 gccataattt tttattgaga ttaatgcccg ccattagcga tccctccagt tcggatgttc
     2641 tgcatcaaga atggattcca gcgcctgaat acgttcgcgc atccgttttg cttcatcagc
     2701 cagttgcgct aatcgctgct gctcactttg cgacaattca ctgcgaccag aacgattgct
     2761 gtaatgcagc cataaccaga tcggtaaaac aaacagcaca aaaatggtta acggaatagc
     2821 cagaaatagc gcgctcataa ggactcctta gacgatgagc ggcggcgcgt tcggacgccg
     2881 cctggatgtt attattgatt gtcttgcttc attttggctt ttaattgtgc cagttgctcg
     2941 ctgattgcat catcggcttt cagttcggca aactgatcgt ccagcgattt ttgtttaccg
     3001 aagctgtggc tttctgcttc cgcttccatc tggtcaattc gacgttcgaa agattcgaaa
     3061 cgagccattg cttcatccag tttgccactg tccagctgac gacgcacatc gcgcgacgag
     3121 tttgccgcct gatggcgtaa catcaatgcc tgctggcgag cgcgtgtttc gctcaatttg
     3181 ttttccagct cgccgatctc tttcttcatg cgtgccagcg tatcgtccac cagcgtcact
     3241 tcatgttcca ggctcttaat cagatcggtc agtttctgtt tttcaattaa cgccgcacgc
     3301 gccaggtctt ctctctcttt cagcagcgcc agttcggctt tttcctgcca ttcaacttca
     3361 cgcgccgacg cttgttcaat acggcgagtc agctgtttct tttctgccag cgcacgcgcc
     3421 gaagtagaac gtacttcaac cagtgtatct tccatctcct ggatcatcag acgcaccagt
     3481 ttctgtggat cttccgcttt ctctaacaga gcgttgatgt tggcattcac gatgtcggca
     3541 aagcgagaaa aaatacccat aatgttgtcc tcttgatttc tgcgttcagg attgtcctgc
     3601 tgaactatta atacaatttg cgtgccaatt ttttatcttt ttgatttata aagatctgat
     3661 tgaagaatca acagcaacat gccaggatga gttagcgaat tacactaaca agtggcgaat
     3721 ttcatcatgg cagaatacaa agataattta ctcggtgagg cgaacagctt tctcgaagtg
     3781 ctggaacagg tttcgcatct cgcaccgctg gataaaccgg tgctcatcat cggcgagcgc
     3841 ggcacaggta aagagctgat tgccagccgt ctgcattatc tctcctcccg ttggcagggg
     3901 ccgtttattt cccttaactg cgcggcgtta aatgaaaatc tgctggattc cgaactgttt
     3961 ggtcacgaag cgggggcgtt taccggtgcg caaaaacgtc atccagggag atttgaacgt
     4021 gccgacggcg gtacgctatt tcttgatgaa ctcgctacgg caccgatgat ggtgcaggag
     4081 aaattattgc gcgtgattga gtacggtgaa ctggagcgcg ttggcggcag ccagccattg
     4141 caggtgaatg tacggttggt atgcgcgacg aatgccgatc tcccggcgat ggtcaatgaa
     4201 ggtacttttc gcgctgacct gctcgaccgg ctggctttcg atgtagtgca actgccgccc
     4261 ctgcgcgagc gcgaaagcga cattatgctg atggcagaac actttgccat tcagatgtgt
     4321 cgggaaatca agctgcctct gttcccgggt tttacggagc gcgccagaga aacattgctg
     4381 aattatcgct ggccgggaaa tattcgtgaa ttgaaaaacg tggtggaacg ttcggtgtat
     4441 cgccacggca ccagcgatta tccgcttgat gacatcatta ttgatccctt taaacggcgt
     4501 ccgcctgaag acgctatcgc cgtttcagaa accacctcgc ttccaacgct gccgctggat
     4561 ttacgtgagt ttcagatgca gcaggaaaaa gagttgctgc aactcagttt gcaacaaggg
     4621 aaatataacc agaagcgcgc ggctgaatta ctggggttaa cctatcatca gttccgcgcg
     4681 ttgttgaaaa agcaccagat ttagcgaagt tgggcaatac aacttgccgg atgcggcgtg
     4741 aacgccttat ccggcataca taatcaatac gttgtttgca ggcctgataa gcgcagcgca
     4801 tcaaacatta ttgcgttggg ctattaatcg ctcagcgcat cctgcaaaat tttcattgcc
     4861 tcatcgaatt gcgcatccgg gatggtcagc ggatacagga agcgaatcac gttgccgtat
     4921 gcgccacagg tcagcaagag cagcccctgc gccagcgcgc gttgctggat tttctgtgca
     4981 atcgccgctg acggctcgcc cgtttgcgga tcgttaaact ctgccgcaat catagacccc
     5041 agaccgcgta ccgccgcgat ggccggaacg ctttctttgg catcaatcaa cgtgtttgtc
     5101 aggcgctggc ccagttgatt cgcgcgttca cagagtgatt ctttgtcgat aatgttgagc
     5161 acagcgtgcg cggcagccac cgccagcggg ttcccggcgt aggtaccacc caacccgccc
     5221 ggcgcgggcg cgtccataat attcgcgtta ccgaccacgc ccgaaagcgg catcccgcct
     5281 gcgaggcttt tcgccatcgt cattaagtcc ggcttatcgg cgtagtgatc catggcaaac
     5341 attttaccgg tacgcgcaaa gccgctttgc acttcatcgg caatcatcac aatgccgtgc
     5401 tcgtcgcaca ggcggcgaat ggcggcaacc agctcttttg gcgcaacgtt gaaaccgcct
     5461 tcgccctgaa ccggttcgaa aataatcgcc gccacctgct tcgcttcaat gtccgattta
     5521 aacaagcgtt cgatggcgtc gagggagtcc tgcgttgaaa tgccgtgtaa atctgacgga
     5581 taaggtacgt gatataccga accggggaac gggccgaagc cgattttgta cggcgcgacc
     5641 tttccggtca gcgccatggt catatatgta cgaccgtgga agccgccgct aaacgcaatc
     5701 acgccagggc gtccggtatg ggcgcgggcg attttcaccg cgttctccac cgcttccgca
     5761 ccggtggtga agaacgcagt tttagcctgc ccgctcaccg gggcaagggc gttaattttc
     5821 tccgccagcg tgacgtagct ttcgtacggc acaatctgat acgccgtgtg ggtaaactgt
     5881 tgcaattgct gttccaccgc cgcgaccaga tcagggtggc gatgcccggt attcagtacc
     5941 gcaatgcctg cggcgaaatc gatatattcg ttgccctcta catccttcag cgtggcgttt
     6001 tctgccgact gggcgaagaa gttacacatc acgccaaccc cgcgtggggt agcagaaaga
     6061 cgacgctgat ggaattcatt gttgctcatg tttcacacct tttgactctt cactgttaat
     6121 cggaaattaa acgcccagac gatcgcgcag gctgtaataa gccgcgccca tcgcggtaaa
     6181 cggaacgcgc agcgtgcgcc cgccggggaa cgggtaatgc ggcagattgg cgaaggcgtc
     6241 gaaacgctcg gcgtcaccgc gcagcagttc agcaatcaaa cgcccggcga ggtgggtaca
     6301 ggtgacgccg tggccgctgt agccctgcat gtagtagatg ttggtatcga ggcgaccaaa
     6361 ctgcggcatg cgtgacaggg tcagcaggaa gttgcccgtc cagcggtaat caattttcac
     6421 gcccttcagt tgcgggaagg ttttcagcag tttcggcacc acaaggcgct caacatcgtc
     6481 cgggtcgcgt gcgccgtaga ccacgccgcc gccgtacagc aggcggttat cggcggtaag
     6541 acggtagtaa tcaagcagat agttacagtc ttccacacag tagtttttcg ggatcagcga
     6601 acgggctaaa tcttccgaca gccgttcggt ggtgatcacc tgcgtgccgc acggcatgct
     6661 gcgtttcgct agttccggct ctactttatc gcccaggtag gcattcccgg cgacaatcac
     6721 atacttcgcc gtcacctgac cattggcagt ccgcaccacg gctggcgtgg tgtgctggat
     6781 ctgcgtcact gcggaaagct catgcacgcg cccgccgttc aggcggatgg catcggcttc
     6841 accaatggcg aggtttagcg ggtgaatatg cccaccgctg tgatccagca gcgcaccagt
     6901 gtagcgatcg ctggcgacct cgcggcgaat agcgttggcg tccagcagct ccagctgttt
     6961 attgccgtag cgttcccagt tctctttctg ctcttcgagt gtggcgagct gtttatcatt
     7021 catcgccaca aacagaccgc cggggcgata gtcgcagtca atctgataac gtttgatacg
     7081 ttcgcggatg atctcgccac cctcgaacat catgctgccg agcatacggg cggtatccat
     7141 gccgtagctt ttttcgatca cgtcgatgtc gcggctgtag gagttcacaa gctgcccgcc
     7201 gttgcgtccg ctcgcgccaa agccgatgcg tgaggcttcg agaaccacca cgtcaaagcc
     7261 cgcttccgcc agatgcagcg cggaggaaag cccggtatag ccgccgccga ccacgcaaac
     7321 gtcgcaggaa atcgactcat tcagcgtgtc gaatggcgcg tatttattcg cactggcggc
     7381 gtagtaactg ctggtgtgtt cggtcatttc aggcctccag gcttatccag atggttttca
     7441 gttcggtgaa tttttcaagg gcatgcaggg atttgtcgcg cccgttgccg ctctgcttat
     7501 agccgccaaa cggcacggtc atatcgccgt cgttgtagtt attgacgaag acggagccgg
     7561 ctttcaggcg gcgactcatg cggtgcgcgc gggagaggtc gcgcgtccat accgccgcgc
     7621 caagtccgta ctggctgtcg ttggcaagct gtagcgcctg ttcttctgat gtgaaacgcg
     7681 tgaccaccag caccggaccg aaaatctctt cgcggcttaa ggacgcattc gggtctacct
     7741 ccacaaagat ggtcgggccg atggcggcag ccagctcggc gttacggcca tccagcagca
     7801 gttgcccttt gctttcgcct tcccgaataa agctatggac cgagtcggcg tgggcgcagt
     7861 cgattaaggt gcccatggtg gttgcgggat caagcggatg gcccggctgc cagttttgcg
     7921 cttgctgttt taacagggct aagaattcat cggcgatgct ctcttccagc aacaggcgcg
     7981 ttccggcgat acacacctgc ccctggttgt agaaaatgcc cgcagcggtc gcgctcgccg
     8041 ctttttgcaa atccggacag tcggcgaaaa cgatgttggc gcttttgccg cccgcttcca
     8101 gccagacgcg tttcatgttg ctgtcgcccg cgtccttcag cagctgtttc ccggtgcggg
     8161 ttgagccagt aaaggcaatg gcatcgatat cgttatggcg cgaaagcgcc tgcccggctt
     8221 catgaccaaa acccgtcacc acgttcaaca caccatccgg cagccccgct tctttcgcca
     8281 gccccgcgag acgaatcgca ctgagcggtg atttttcaga cggttttaga atcacgctgt
     8341 ttcccgccgc cagcgccggg ccgagtttcc agcaagtcag cagcagcggg aagttccacg
     8401 gcacgatggc ggcaatcacg ccgaccggtt cacgcacgat catcgccagc tcatggctac
     8461 tggtggtcgc cacttcgcca tacactttgt cgatcgcttc ggcgtaccag cgaatggcgc
     8521 gcgccgcgcc gggaatatca tcacgcaggc tgtgacgaat cggtttaccg gtgtcgagcg
     8581 tttccagcag cgccagttct tcagcgttgg cttccattaa atcggcgagt ttattcagca
     8641 ccgctttacg cttagccgga gaagagagtg accagtcgcc acgttcaaac acgccgcgtg
     8701 ctgcgctcac cgcacggtca atatcgacgc tcttgccgcg ggcaatattc gccagcggtg
     8761 cctgggtgac cggatcaacg gtttcaaaag tttcattttc cgccgcagca gtatattcac
     8821 cgttaataaa taagcgggtt tcaatggcga gacttaacgc tttatcctgc cagtaagcca
     8881 gatgatgaaa attcattatg actcctgttt cacgtctatc agatatatgc agtttgtgaa
     8941 cgtcagggcg cagagatccc cgggccgtat gcccgtttat ttctggcgac attctttata
     9001 gattgccggg cgcattgcgc ttacccggct tacagaacaa aaagattaaa acgtggtggg
     9061 cgtatgggcg ctgataattc ggcaaatacc tgccgacgta ttactgaaac tgtgcgggat
     9121 gccggtatta atggcataac tttgccccgc gacgagatga taatcctgac cattaatcgt
     9181 cagaacaatt tcaccttcca gtacagtgcc tatttcctca ccctgatgct taattctttc
     9241 cccagtggtt gtgcccggct ggtaagtttc aaagatcatc gccagcgtgc gattcgggtt
     9301 accgttatga accagcttca ttgacacacc ctgactgccc atctcaatta agtcgtcctg
     9361 attaatgacg acctgcggct catcaggttt ttccggctcg gaaaagaatt ccgagagtga
     9421 cagaccatac accttcagca gcttttgcag cgtactgatg gcagggctga ctttatcttg
     9481 ttctatcgta ctgatagcac tgtgagtcag cccggagagt tcggcggcac gacgttgtga
     9541 aagcccctgc tgctggcgga tttccgacaa gcgttttcct ggcgccagtc cctcatcact
     9601 catatttgca tttccttaaa ctgtagtggt cagagtcgct gtttttcagc gatatggtgc
     9661 tgacaagcgg tgataaagcc ctcgaacaat atacgcgaaa gcgcgtactc gctactgttc
     9721 cattccgggt gccactgtac gcccagcgca aaaggatgat tgatgacgct aaccgcctca
     9781 actaagccat ccggcgagcg agcttcaaca cgcaaccgtg ggctaacgac cttcgcccct
     9841 tgtccatgca gagagtttac ccaaaagttg ctacattcag gtaacaacgc agacagtaat
     9901 cccccctctt caacctgaac ttcatgcgac ggcgcatatt gctgctccac cggcagttcg
     9961 ggatcttccc gatgttccag caattcaggc tgttcgcaca gcttgcgatg caacgaacca
    10021 ccggttgcca ccaccagttc ctgtaaaccc cggcagatgg cgaaaatggg gatgcgcctt
    10081 tcgagtgcgg cattaattaa cgccatgctc agaagatcac gcccaggatc ggcgtcaggc
    10141 tcatcgccgt tttcaccata taggtgcggc tgcacattgc tgggactacc aggaagataa
    10201 atgccatcga gtttcggcaa aagttgttca agtaatgacg gttccgccag cgcatgtggc
    10261 agcgcaattg gcaagccgcc tgcgtggatg atggcattca ggtacttttc ttgcagagtc
    10321 tgggtcgcat gacccttaag cctgttcctg cacattacga caccgataac cggattgttc
    10381 attatatttt ccatgctcaa tctcacaaag tggactaaat tatcgccatt actgcgcttt
    10441 aaatgacctt tatgttcaat attttttcaa tctagcagtg ggttttcatt tttgcaaact
    10501 caatttaaca tttgacaaac atttagtttg catacagatt cgaatagtgg tcattatatt
    10561 ttacgctttg ataacgagcg gaaaacaaac caaaggcgaa gaatcatgga aaccaatatc
    10621 gttgaagtag agaactttgt tcagcagtca gaagagaggc ggggtagctc ctttacgcag
    10681 gaagtgaaac gctacctgga gcgctacccg aatacgcaat atgttgatgt tttacttacc
    10741 gatttgaatg gttgcttccg tggaaaacgt attcctgttt caagcctgaa gaagctcgag
    10801 aaagggtgtt atttcccggc ctcggtgttt gcaatggata ttctgggcaa cgtagtagaa
    10861 gaagcgggcc tgggtcagga aatgggcgag ccggatcgta cctgtgttcc tgttctcggt
    10921 tccttaaccc cttctgccgc cgatccagag tttatcggtc agatgctcct gaccatggtc
    10981 gatgaagatg gcgctccctt tgacgttgag ccgcggaacg ttcttaaccg cctgtggcag
    11041 cagctgcgcc agcgcggatt gttcccggtc gtagcggtag agctggagtt ctatttactg
    11101 gatcgccagc gcgacgctga aggatatctg caaccgccct gcgcgcccgg caccgatgac
    11161 cgcaatacac aaagccaggt ttactccgtt gataacctca accacttcgc cgacgtgctc
    11221 aatgatattg atgagctggc gcagttacag ctgattccgg cagatggcgc ggtcgccgag
    11281 gcctcgccag gtcagtttga aatcaatctt taccataccg ataacgtgct ggaagcttgc
    11341 gatgatgcgc tggcactaaa acgcctggtg cgtctgatgg cagaaaagca taagatgcac
    11401 gccactttta tggcgaagcc gtatgaagag cacgcgggca gcgggatgca tatccatatc
    11461 agtatgcaaa ataatcgtgg cgagaacgtg ctttctgacg cggaaggcga agattcgccg
    11521 ctgctgaaaa agatgctcgc cgggatgatt gacctgatgc cgtcatcaat ggcgttgctg
    11581 gcaccaaacg tgaactcgta tcgccgcttc cagccgggga tgtatgtacc gacgcaggcg
    11641 tcgtggggcc ataacaaccg caccgtcgcc ctgcgtattc cctgcggcga tcgccataat
    11701 caccgcgtgg aatatcgcgt ggcgggtgcc gatgccaacc cgtatctggt gatggcggcg
    11761 atttttgccg gtattttgca tggccttgat aacgaactgc cattacagga agaagtcgaa
    11821 ggtaacgggc tggaacagga aggcttaccc ttcccgattc gccagagcga tgccctgggt
    11881 gagtttatcg agaatgatca cctgcgccgc tatttaggcg aacgcttctg ccatgtctat
    11941 cacgcctgta aaaatgatga gctgttgcag tttgagcgcc tcatcacaga aacagaaatt
    12001 gagtggatgt tgaaaaacgc gtaatacccc ttgcggggtt gtttcatcgg cctgagcgga
    12061 gccaggcctg acatttgctg gtaatccaga catttcccgg atgtcgcgtt atggagcgca
    12121 ggcggcaacg ggcttccgtt aacgattaag actgtgacga ggaaatgaga tgatgccgat
    12181 gattaaatcc ccgcatggcg agggaggctg cgtatgcgcc cctcctgcga ctgactggac
    12241 tccgccccct ctgcttccct tgctaaacag gtttgatttc aggtcgacac gaccgcaaac
    12301 cttattacgc aggggaggca gcaattatgg ctattaattc accactgaat attgctgcgc
    12361 aacctggcaa aacccgtctg cgaaaatcac tgaaattgtg gcaggtggtg atgatgggtc
    12421 tggcctatct cacgccgatg accgtatttg atacttttgg tattgtctcc ggcattagcg
    12481 acggtcacgt tccggcgtcc tatttgctgg cgctggcggg cgtgctgttt accgctatca
    12541 gctacggcaa actggttcgc cagtttccgg aggccggttc ggcctatacc tacgcgcaaa
    12601 agtcgattaa cccgcacgtc ggatttatgg tcggctggtc atcgctgctg gattatctct
    12661 ttttgccgat gatcaacgtt ttgttggcga aaatctatct ctccgccctc ttcccggaag
    12721 tgccgccgtg ggtgtgggtg gtaaccttcg ttgccatttt aaccgccgcg aatctgaaga
    12781 gcgtcaacct ggtcgctaac ttcaatacct tgtttgtact ggtgcaaatc tccatcatgg
    12841 tggtgtttat cttcctggtt gttcagggac tgcataaagg agaaggcgtt ggcaccgtct
    12901 ggtcacttca gccgtttatc agcgagaacg cgcacctgat cccaattatt accggggcga
    12961 cgattgtctg tttctccttc ctcggtttcg atgcggtgac cacgctttcg gaagagacgc
    13021 cggacgccgc acgcgtgatc ccgaaagcca tcttcctgac ggcggtatac ggcggcgtta
    13081 tctttatcgc tgcgtcgttc tttatgcagc tgttctttcc cgatatcagc cgctttaaag
    13141 acccggacgc cgcactgcct gaaattgcgc tatacgtcgg cggcaagctc ttccagtcga
    13201 ttttcctctg taccacgttt gtgaacacgt tagcgtctgg cctggcgtcg cacgccagcg
    13261 tgtcgcgtct gctgtatgtg atggggcgtg acaatgtgtt tccggagcgc gtgtttggct
    13321 atgtgcaccc gaaatggcgg actccggcac tgaacgtcat tatggtcggg attgtcgcgt
    13381 tgtcagcgct gttctttgat ttagtcaccg cgacagcatt gattaacttc ggtgctctgg
    13441 tggcatttac cttcgtaaat ctgtcggtgt ttaatcattt ctggcggcgt aaaggaatga
    13501 ataaaagctg gaaggatcac ttccactatt tgctgatgcc gctggttggc gcgctgacgg
    13561 tgggtgtgtt gtgggttaac ctcgagtcaa cgtcactgac actcggtctg gtatgggctt
    13621 cgttaggcgg cgcatatttg tggtatctga tccgccgcta tcgcaaagtg ccgttgtatg
    13681 atggtgacag aacgccggtg agcgaaacgt aatagtcgga tgcgcggcgc attatcctgc
    13741 ctgagggtaa tgcaccgcgc cattaactat aaaaagatgt atgacccgga tcacgagtgc
    13801 tatcatccgc ccttatgctt agcgcattaa ccccaggggc aaataatgaa ccacgatatc
    13861 ccgctaaaat attttgatat tgccgatgag tacgcgaccg aatgcgcaga gcctgtcgca
    13921 gacgctgaac gcacaccgct ggccctctac ttccagttac tgctcacccg tttgatgaac
    13981 aatgaagaga tcagcgaaga agcccaacat gaaatggctg ctgaagcggg gattaatccc
    14041 gtacgcattg atgagatcgc agagtttttg aatcaatggg gtaatgagta attatttcat
    14101 tgcagactct cggtcagtat cgttgtccgg ataaagcgtt cacgccgcat ccggctaaca
    14161 atgacacgca ctgcagcgtg cctcaatggg tttctcacga tcgtaccgct ctgctcctcg
    14221 ctaagttgtt ctgtgagtta tagttataga gaaatcacgg gtacgaatgt tgaacagatg
    14281 gcgacgtcat ctgttcaacg accaaccaca cggccctcat tacgacccct gaacacaaac
    14341 ggtaatggca aatgtcatta aatattagta agatagcctc tttacataaa atccattaat
    14401 attatgcgcc aggtattatc gtctcttttg gtgattgctg gacttgtgag tggtcaggca
    14461 atcgccgcgc ctgaatcccc cccgcatgct gatatccgcg acagcggttt tgtctattgc
    14521 gtcagcgggc aagtcaacac ctttaaccca tccaaagcga gcagtgggtt aattgtcgat
    14581 acccttgccg cccagtttta tgatcgactg ctggatgtcg atccctatac ctatcgcctg
    14641 atgccggaac ttgccgaaag ctgggaagta ctcgacaacg gcgcgaccta tcgcttccac
    14701 ctgcgtcgcg atgttccgtt tcaaaaaacc gactggttta ctcccactcg taaaatgaat
    14761 gccgacgatg tggtgtttac cttccagcga atttttgacc gcaacaaccc gtggcataac
    14821 gtcaacggca gcaacttccc ctacttcgac agcctgcaat ttgccgataa cgtgaaaagc
    14881 gtccgtaaac tggataatca taccgttgag ttccgtctgg ctcagccgga tgcttctttt
    14941 ttgtggcacc tcgcaaccca ttatgcttcg gtcatgtcgg cagaatatgc ccggaagtta
    15001 gagaaagaag atcgccagga gcaactcgac cgtcagccgg tcggcactgg accgtatcag
    15061 ttgtcggaat accgcgccgg gcaatatatt cgcttacaac gtcatgatga tttctggcgt
    15121 ggtaagccgt tgatgccaca agtggtggtg gatttaggct ctggcggtac aggacgattg
    15181 tcgaaactcc tgaccgggga gtgcgacgtt ctggcctggc ctgctgccag tcagctatcc
    15241 attttgcgtg acgacccacg cttgcgttta acgctgcgcc cggggatgaa cgtcgcctat
    15301 ctggcgttta acaccgctaa accgccgcta aataaccccg ctgtccgcca tgcgctggca
    15361 ctggcgatta ataaccagcg cctgatgcaa tccatctatt atggtacggc tgaaacggcg
    15421 gcctctattt taccgcgcgc ctcgtgggcc tatgacaacg aggctaaaat tactgaatac
    15481 aatccggcaa aatcgcgcga acagttgaag gcgttggggc tggaaaactt aacgctgaaa
    15541 ctgtgggtgc caacacgttc gcaggcgtgg aaccccagtc cgctgaaaac cgccgaactg
    15601 attcaggcgg atatggcaca ggttggcgta aaagtggtga ttgtgccggt agaaggtcgt
    15661 tttcaggagg cgcggttgat ggatatgagc catgatctga cgttatccgg ttgggcgacg
    15721 gacagtaacg acccggacag tttcttccgt ccgttactga gctgcgcggc aattcattca
    15781 cagaccaacc tcgcccactg gtgcgatccg aaattcgaca gcgtgttgcg taaggcgctc
    15841 tcctcgcagc agctggcggc gcgtattgaa gcctatgacg aagcgcagag tattctggcg
    15901 caggaattac ccattctgcc gctggcgtcg tcattgcgct tgcaggccta tcggtacgat
    15961 atcaaaggtc tggtacttag cccgtttggt aacgcctcct ttgctggggt gtatcgcgag
    16021 aaacaggatg aggtgaaaaa gccatgatta tcttcacctt gcgccgtatt ttgttattga
    16081 ttgtcacctt gttcctgctg accttcgttg gcttcagctt gagctatttc accccacatg
    16141 cgccgttaca aggcgcgtca ctgtggaatg cctgggtgtt ctggtttaac ggcctgatcc
    16201 actgggattt tggtgtttcc agcattaacg gtcaaccgat tgccgaacag ttaaaagagg
    16261 tcttcccggc gacgatggaa ctgtgcatcc tcgccttcgg ttttgcgctg attgttggca
    16321 tccccgtagg aatgattgcc gggattacgc gccataagtg gcaggataac ttgatcaacg
    16381 ccatcgcctt actgggcttt tcaatcccgg tgttctggct ggcgcttctg ctgacgctgt
    16441 tttgttcact cacactaggt tggttgcccg tttcagggcg tttcgatctg ctctacgaag
    16501 tgaaaccgat taccggtttt gcgttgattg atgcctggct ttcggactca ccatggcggg
    16561 atgaaatggt catgagcgca atccgccata tgatattgcc cgtgatcacc ctgtcggttg
    16621 cgccaacaac tgaagtgatc cgactgatgc gtattagcac tatcgaagtg tatgaccaga
    16681 actatgttaa agcagcggcg acccgcggtt tgtcgcgctt tactattttg cgtcgccacg
    16741 ttctgcataa cgcgttacct ccggttattc ctcgcctggg cttacagttt tctaccatgc
    16801 tgacgctagc gatgatcacc gaaatggttt ttagctggcc gggtttaggg cgctggttaa
    16861 ttaacgccat ccgccagcag gattacgcag ccatttccgc cggagtgatg gtgtgtggct
    16921 cactggtcat tattgttaac gtgatttctg atattttggg tgccatggct aaccctctga
    16981 aacataagga atggtatgcc ttacgatagc gtatacagcg aaaagcgccc gccgggcacg
    17041 ctgcgtaccg cctggcgcaa attttatagt gacgcctctg cgatggtcgg cctgtacggc
    17101 tgcgcggggc tggctgtact gtgtattttt ggcggctggt ttgcgcctta cggcatcgac
    17161 cagcaatttc tcggttacca attattgccg ccgtcatggt cgcgctatgg cgaagtttct
    17221 ttcttcctgg ggactgacga cctcggacgc gatgttttaa gccgtttact gagcggagct
    17281 gcgccaaccg taggtggcgc atttgtggtg acgcttgccg cgacgatctg tggcctggtt
    17341 ctgggcacct tcgccggggc gacccacggc ctacgctcag cggtgcttaa ccatattctg
    17401 gataccttac tggcgatccc ttcgctgctg ctggcaatta tcgttgtagc gtttgccgga
    17461 ccgagtttgt ctcacgccat gtttgctgtc tggctggcat tgctgccgcg tatggtacgt
    17521 tcgatttaca gcatggtgca tgacgaactg gaaaaagagt acgttatcgc cgcccgtctg
    17581 gatggcgcat caacgctgaa tattctctgg tttgctgtga tgcccaacat caccgccggg
    17641 ctggtcaccg agatcacccg cgcactgtcg atggcaattc tcgatatcgc cgcgctgggc
    17701 tttctcgatc tcggcgcaca actcccctcg cctgaatggg gagcgatgct cggtgatgcg
    17761 ctggaactga tttatgtcgc cccgtggact gtcatgctgc caggtgcggc aattatgatt
    17821 agcgtgctgt tggttaacct ccttggcgac ggtgttcgtc gtgcaattat tgcgggggtg
    17881 gaataatgcc attactcgat attcgtaacc tgaccattga atttaaaacc ggtgatgagt
    17941 gggttaaagc cgtcgaccgc gtaagtatga ccttaaccga aggtgaaatc cgcggtcttg
    18001 ttggcgaatc cggttccggc aaaagtttga ttgcgaaagc gatttgtggg gtgaataaag
    18061 ataactggcg tgttactgct gaccgtatgc gttttgatga tatcgatttg ctgcgtctct
    18121 ccgcacgcga acggcgcaag ctggttggtc acaacgtgtc gatgattttc caggaaccgc
    18181 agtcttgtct tgacccttca gaacgtgtgg gtcgccagtt gatgcaaaac atcccagcct
    18241 ggacgtataa aggccgttgg tggcagcgtt ttggctggcg caaacgccgt gccattgaac
    18301 tactgcaccg tgtggggatt aaagatcaca aagatgcgat gcgcagtttt ccctatgagt
    18361 tgaccgaagg tgaatgtcag aaagtgatga tagccattgc actggcgaat caaccgcgtc
    18421 tgctgattgc tgacgaaccg acaaactcaa tggagccaac aacccaggcg caaatctttc
    18481 gcctgctgac gcgtctcaac cagaacagca ataccactat tttgcttatc agccatgact
    18541 tacaaatgct tagccaatgg gcggataaaa ttaacgtgct ttactgcggt caaacggtgg
    18601 aaaccgcgcc aagtaaggag ttggtgacaa tgccacatca tccttatacc caggcgctga
    18661 tccgcgcgat accagacttc ggcagcgcga tgccgcataa aagtcgcctc aatacgctgc
    18721 ccggcgctat cccactgctg gaacagttac cgattgggtg tcgtctgggg ccgcgttgcc
    18781 cgtatgcaca acgagaatgc attgtgacgc cacgtttgac gggggcgaaa aatcatctct
    18841 atgcctgtca cttcccgctg aacatggaga aagagtgaga tgatcgaaac gctgcttgaa
    18901 gtgcgtaatt taagtaaaac gttccgttac cggaccggct ggtttcgtcg tcagaccgta
    18961 gaagcggtaa aacctttgag ctttacgcta cgtgaaggcc agacactggc gattattggt
    19021 gagaatgggt cgggtaaatc cacactggca aaaatgctgg cgggaatgat agaaccgacc
    19081 agcggcgagt tattgattga cgatcatccg ctgcattttg gcgattattc cttccgtagt
    19141 cagcgtattc gcatgatttt tcaggatcct tcgacctcgt tgaatccccg tcaacgtatc
    19201 tcgcagattc ttgattttcc actgcgcttg aacaccgatc tggaacctga acagcgtcgc
    19261 aaacagatta ttgaaacgat gcggatggtt gggctgctac cggatcacgt cagttactat
    19321 ccgcatatgt tggcgcccgg acaaaaacaa cgtctgggtc tggcacgcgc gctgatattg
    19381 cgtccaaaag tgattattgc cgatgaagcc ctcgcctcac tggatatgtc gatgcgttcg
    19441 cagttgatta atctgatgct ggaattacag gaaaaacagg gtatttcgta tatttatgtt
    19501 acccagcata tcggaatgat gaagcacatt agcgaccagg tactggtgat gcatcagggc
    19561 gaggttgtcg agcgaggcag caccgccgat gtgctggcat cgccgctgca tgaactcacc
    19621 aaacggctta ttgccggtca ttttggcgag gcattgacgg cggatgcgtg gcgtaaggac
    19681 cgttagtggt ttgcacctca tcctgacagg gcccccctca ggatgaggtg ttcattactt
    19741 ttgattgtcg attctggtga gagcaaccag caatgccagc gtataacaca ccgtaattgc
    19801 cagactcatt tttaacagtg tttctccgcc aattcccgac aatggcgcgg ctataccgcc
    19861 gaagacaaac atgctcatcc ccatcaacgc agacgccgtt ccagattcct gcgtccctac
    19921 tgcactcata gcctctgccc cgccaacagt gccgatgccg ctacaaaatg cgatggtgaa
    19981 aaataatgcc accagtgccg gaatgggcaa ttgtatccat gcgcataaga ccgtgagcaa
    20041 tgcacacaaa attgccgcta tcaggccacc tcgcaacaat gtcatcgcgt taatccgtcg
    20101 cgccaggcgc gagaagatcc atgaagcaat gataagtccg atgccgttaa ggccaaacac
    20161 caggctaaat tgcattggac taaagccaaa ttccttctgc aacacgaacg acgaagagcc
    20221 gatatatgca aacaaaccag ccagcataaa actttgaatc aggcaaaagc gtataaaacg
    20281 gcggttctgc accacgcttc gtccggttaa tagcaatgat gagccccttt tattttctgg
    20341 caaggtctca ttaataaata acaggcagcc cagcaacagt acggtgctaa tttcagccat
    20401 tacccagaat aaagtgcgcc agtcaaaagt gctaacaatg tacccgccca gcactggcga
    20461 caacaccggt gccaggccat tcaccgtcat taacagcgca aaaaactggg tcaacgttac
    20521 tccctgatat ttgtcacgag caatagaacg agagagcacc gaaccacccg cccccgcgat
    20581 cccttgaata aaacgccaga ccaccagcca gtaaatattg ttcgttgtcg cgcataaaat
    20641 ggaagaaaca ataaacaata gcaacgacaa gattaacgga cgtttacgcc ctattttgtc
    20701 gctcagaggg ccaaataaca gttgtccgac gccaagacca atcagtgatg cggtcagggt
    20761 taattgcgtt atggtcgtgg ttgccgccag ttgctcagac atctccggca aagccggcaa
    20821 ataaagatcg gtacacatcg ggccgatacc ggctaaaaga ccaagaatca atgcccatga
    20881 aagagagact ctagccatat tattccttac ttgagtgatg acataccgcc gcccagcgac
    20941 tgccagagcg tgattcggtt tgtaaaatca gtcagttgca gtgccagcag ctcctgctgt
    21001 gcgctccaca agctgcgctg cgccgtcaac acggtcaggt aatcaccaac acccgcctga
    21061 tagcgacgca agccaacatc gaccgtttgt tgctcagctt taacatactg gcgctgtgca
    21121 tccagttgct cttcaagcgt ggtccgccgc gccagtgcgt tagcgacatc tttaaatgcg
    21181 ctctgaacgg ttttttcata ggtggcaatg agcccacgct tttgtgcttc tgcgtaacga
    21241 agctgcgcca gattacttcc gccagtgaac agcggtaatg tgaccgatgg cgcaaatgac
    21301 cagatctgca ttccgtggct gaacaacgac gacaatgcgt cactgccaac accggcactg
    21361 gcagtcagcg aaatcgtcgg gaaaaagtta gcccgcgccg cgccaatatc tgcattggcg
    21421 ctcttcaaat tatgttcagc ttcctggata tccgggcgac gcagcagaac atcagatgat
    21481 actcccgcag gaaccagact tatcatttgt tccggcaggc tttccagtgt tcccggtagc
    21541 agattttccg caagcgttgt acctgccagt aagttcagcg catttttatc ctgcatcact
    21601 tgtgtctgat agctggcaac gctggcgcgc gcttgctgat aaacactcat cgcctcactg
    21661 acatctgtcg ctgccgccgt gccaatctgt tgctggcgct ggataatttt caatgagttt
    21721 tctgcactgg tcatcgtttc ttttgccagg gccaggttgc tgttatcagc cgccagtgtc
    21781 agccaggcgg tgctgatttc agcaatcagc gttaaacgag tgttctgggc tgtaaattca
    21841 ctggcaagcc aggtttcctg cgccgcccga gataaactct gattacgacc aaacagatct
    21901 aacgtataac tggacaccgt gccgtcagcc tcggcagttg ttcccgtacc attcgccagc
    21961 gaacgactgc gagtcgaact taaggccgca ttcaccgtcg gaaataacga cgcattagtt
    22021 tgcccataga gcgcccgtgc tgagtcaata tcagcaatcg ctttttgcac atcgcggtta
    22081 ctgttcagtg caatggttac tacctgctgc aatcggggat cgtggatcac ctgctgccag
    22141 tcatggctga ttgccttccc ctggccctgt gctcccggca aggtcgccgg gattggggat
    22201 tcaggcgtac tgtagtgggg atcgagcgag acacatcccg cactcaacaa caccagaaaa
    22261 ataagagaac gacgcaacat aacttactcc tgacggcgcg gtttaccggc aaacaaacgt
    22321 ttcaccagta caaaaaacag aggaacaaaa aaaatagcca gtaatgtagc ggtcagcgtg
    22381 ccgccaataa tgcccgtacc aatggcgatg cggctgttcg cccctgcacc ggttgctatc
    22441 gccagcggca ttacccccgc aataaacgcc agcgaggtca tgatgattgg gcgtaaacga
    22501 gtctgcgccg cgcgtaatgc cgcacgactc aaggaatagc cctctgcaac cgccgcttca
    22561 gcaaattcca caatcagaat ggcgtttttc gacgacaagc cgatagtggt taatagcgcc
    22621 acctggaagt aaacatcgtt gtttaaatca cgcatccagg ccgccagcgc cgcgccaaga
    22681 agccccagag ggatcaccag aatgaccgag aacgggactg accagctttc gtacagtgct
    22741 gcaaggcaga ggaaaaccac cagaatagaa actgcataca gactcaatgc ctgaccgctg
    22801 gccagtttct cctgcaatga caaaccactc caggcccagg ttgttcccgc aggaagactg
    22861 tttgccagtt cctccatttt tgtcattgca tcgccggaac taaagccagt cgcattttcc
    22921 ccctgaattt cataggctgc agaaccgttg tagcgcacca gtctttccgg tccatacagc
    22981 cagcgggtgg tcgcaaaagc agagaatggt gtcatggcgt tatcgctacc gcgcacaaac
    23041 catttaccta agtctgacgg cgcggaacga aattcactgt cgccctgaat gtagactttt
    23101 ttcacacgac cacggtcaat aaagtcattc acataagtac cgccccacgc actggacagg
    23161 gtgtcggtga catcattcaa actcaaccct aatgacaccg ctttattact atcaatatct
    23221 acctgcaatt gcggcatttg tggcaaatca ttggcacgca cagaatgtaa ctccggactt
    23281 tgattcgctt tttctatcaa ctgattacgc atttgcagta gtgtttcgcg atcggttcca
    23341 ccgttagcta acaattcaaa cgtaaaacca ttgctttggc ccagcccatc aacggctggc
    23401 ggcgtcatcg caaataccgt ggcatcacga attgtgccca gctctttggt agcccgtagg
    23461 gcgatagcct gggcggtgtt ttctgcccct ttacgttgag accagttttt caacgaaaca
    23521 aacgccatcc cggtgttctg tccgctgccg ctaaaactga aaccatcaac ggtaaaaatg
    23581 acatcggtat ttgctttctc gttaatcagg aaccagtcaa caatctggcg attcacttct
    23641 gctgtacggg cagcggtcgc cccggccggc agcgtgtact gcaccatgat ttcgccctgg
    23701 tcttcagtgg gtaagaaact gcccggcagt ttccacatca tcagggccat tccaccaccc
    23761 agtacgacat aaagccccat cgttcgagca gcacggcgca ggacataaat tacgcctcgc
    23821 tgatatttat cttcagtacg gcggtaaaag cggttaaatg cgccgaaaaa gcctttttta
    23881 tgtggcggaa catgctggag gacggaaccg cacagggcgg gagtcaaggt caatgccacc
    23941 accacggaaa gcagcattgc ggagatgatg gtgatggaga actgacgata aattacgccc
    24001 gtagatcccc caaagaaggc catcggcagg aatacggctg acaacaccag cgcaatggca
    24061 accagtgcgc cagaaatctc gcccattgat ttttccgtgg cttcacgcgc gggcaaccct
    24121 tcgtcacgca taatgcgttc gacgttctct accaccacaa tggcatcgtc caccagcaag
    24181 ccgatcgcca gcaccattgc aaacaacgtc agggtattaa tcgaatagcc aaacaacgca
    24241 agaacgccga atgtgcccag caaaacgacg gggacagcta acgccggaat cagtgtggca
    24301 cgcaggtttt gcaggaataa atacatcacg caaaccacca atacgatggc ttcaaacagc
    24361 gtctgaatta cgtcctctac ggagatttta ataaattcag tgctgtcttt cgggtatgcg
    24421 atgtcgtatc cctgcggcat gttccgctgg aattcggcaa tcttatcctt gaccagcgtc
    24481 gccgtattca gcgcattcgc accgggcgaa agcattacag caaccccggc agacgggtga
    24541 ccgttaagtt tgccgatagc ggtataatct tcactgccca tctcaacgcg agccacatct
    24601 tttatacgaa caactgcgcc gtctgactgg cttttcacga taatattttt gaactgatcc
    24661 accgtctgca aacgagactg cgcacgtacc gttgcggtca gttgctgagt attcggtgaa
    24721 ggcaatgccc ctattttccc ggcagtaacc tgcacgtttt gcgcttcaat agcactttgc
    24781 acgtctgaag gcattagcga gtaagacgcc agtttggccg ggtcaagcca gatgcgcata
    24841 gcgtattccg cgccaaagac ttgcagactc cccacacctt caacacgcgc cagcgggtcc
    24901 tgaacgttac taaccaacca gtcggcgata tccgaactgg aggctttgtc agtggtgtca
    24961 tacacggcag caatcagcaa aaagttgctt tgtgatttct ccaccgtaac acccgtttgc
    25021 tgcacttcgc tgggtaggcg cgactccgcc tgctgaattt tattctgcac ctgaacctgt
    25081 gcagtatctg gatcggtacc ttgttcaaag gtcacattaa tactgaccga accatcagag
    25141 ctgctggttg agctgaaata gagtaaatta tcaagcccag tgagttgttg ctcgataacc
    25201 tgagtcacgc tgttctccag cgtttcggca gaagcaccag tataagtggc tgaaatttta
    25261 atggtcggtg gcgcaacgtc aggatattgc gcgacgggca atgtgcgaat agccagaatc
    25321 cccgccaaca taatcaaaat ggcgataacc caggcaaaga ccgggcggcg aacaaaaaag
    25381 cgcgaaaaca tcaggcgttg cctccattag cctgaacttc aacagccttg acggtctgcc
    25441 ctgaagtgac tttggcagaa ccttcaacaa tcagtcggtc gccgttgtgc aggccgttca
    25501 gcaccagcca tttatcacca tacgtttctc ccgtttcgag cgttcgctgc tctactttat
    25561 tgtctttatt caccaccagc gcagttgcat tgcctttagc atcgcgcgtg acgccctgtt
    25621 gcggcgcgag aatagcgtct tccatcacgc cttcatcgac acgagcgcga acaaacattc
    25681 ccggtaataa ctgctgttgt ggattgggaa aaatcgcccg taatgtcacc gaaccggtag
    25741 actcatctac agcgacttcg gtgagttcca gacgcccttt ttcgctgtaa gttgtgccat
    25801 cttccagaat aagtgagacg ctcatggtgt cactgttggt cgccagtgac tgtttacgca
    25861 gacgtaataa atcgacacta gagcgagtga ggtcgacata cattgtatcc agaccacgaa
    25921 tagtcgttaa cgctgtatcc tgcgacgcgg tcaccagtgc gccaggggtt accgacgaaa
    25981 taccaatacg ccccgaaatc ggtgcggtta ccgtggtcca gtcaagatta atgcgcgcag
    26041 tttccagtgc ggcttttttc gcctctacac tggctttatc ttgtgcacag gtagactgag
    26101 catcatcagc atcctgttgt gaaacaccgt tctctttcac cagtcgggca taacgctgcg
    26161 ctttctggca atcggctttt accagtgcct gtgcttgttg taatgctgcc cgagcttcat
    26221 tccatgcagc ctgataactg gccgcatcaa tctggtagag cggctgtcca gccttgacca
    26281 gatcaccttc cttaaataag cgtttctgga taattccccc aacctgcgga cgtacttcag
    26341 cactgagcgc agcactggtg cgtccggtta attcactgac taccgaaacc ggttgagatt
    26401 tgagcgtaac aacgccgact tctgtttctg acggggatga attgttactt tgcgtattat
    26461 cacaacccga taagagcagc attgccacta cagatgttgc tatatacttc attattttct
    26521 ccagggtgag aaataccctc ctcattgaaa ttaatgagga tatgtcaggg atattcagtt
    26581 ataacgaacc cgcatcgcga aatgaatttc gcaatgaaat aagggggatt aatatccccc
    26641 gctattggtt aattctgttc ttatttattt atcaacatac taacaatatc ctgatatatt
    26701 tcctgcagat gttgtggatc gcttttttgc ggtgttaaac gacgataaac cgtaccttcc
    26761 atcatggtag caaacacttc aacgcagcaa cgaatgtgct cgtcgctaag atggggatgc
    26821 attcttttca tatgtgcaca accgttagcg aacattctgg cgtcagcttc ttcaagcatt
    26881 gccataaccc gcgggttgcg tgtggcttct gcagccactt ccagcatcag cgcgtcgtcg
    26941 tcctcattta aggatttacg caaggcaagg acttccggga agtgatcggt acgcgcgtca
    27001 atgtccattt gggcaatacg aaagtcgatg atacggcgaa ccatctcttc aataatggcg
    27061 tctttgttgg cgaagtaacg atatatctga cccacgctaa gctgagcttc gctggcaatt
    27121 tgcgacatac tggcggcatg aaatccgcag cgacgaaaac accgacgggc ggcgttaatg
    27181 atttcatcct gacgctgttt gtggcgtatc tccagcttgc tggtcatttc tgtctcctgg
    27241 cctatcactt gatgagaatg atcgttctca tttttgctaa ttatacgttt ctccaaagta
    27301 atggaaagta tttttatctt attcatgatg catattctga taatgaagag attgtggaga
    27361 ttctgtgaag atagttgaca atattaagtt ggggtcaatt catcagaaag acagcgactt
    27421 gttgtcgcca gttctgtttt catcatcaaa tttatcggag ctaatacgaa tagtttcata
    27481 gtcagattct ataagatatt tccccaacct cccccttgca ggcgtcagga gcagataacg
    27541 caaaatgctc tgtaccgcag cttctctccg ctacagaaag cgcaactata gccacccaca
    27601 gccaggttga ttataataac cgtttatctg ttcgtactgt ttactaaaac gacgaatcgc
    27661 ctgattttca ggcacaacaa gcatcaacaa taaggattaa agctatgggt tttctttccg
    27721 gtaagcgcat tctggtaacc ggtgttgcca gcaaactgtc catcgcctac ggtatcgctc
    27781 aggcgatgca ccgcgaagga gctgaactgg cattcaccta ccagaacgac aaactgaaag
    27841 gccgcgtaga agaatttgcc gctcaattgg gttctgacat cgttctgcag tgcgatgttg
    27901 ccgaagatac cagcatcgac accatgttcg ctgaactggg gaaagtttgg ccgaaatttg
    27961 acggtttcgt acactctatt ggttttgcac ctggcgatca gctggatggt gactatgtta
    28021 acgccgttac tcgtgaaggc ttcaaaattg cccacgacat cagctcctac agcttcgttg
    28081 caatggcaaa agcttgccgc tccatgctga atccgggttc tgccctgctg accctttcct
    28141 accttggcgc tgagcgcgct atcccgaact acaacgttat gggtctggca aaagcgtctc
    28201 tggaagcgaa cgtgcgctat atggcgaacg cgatgggtcc ggaaggtgtg cgtgttaacg
    28261 ccatctctgc gggtccgatc cgtactctgg cggcttccgg tatcaaagac ttccgcaaaa
    28321 tgctggctca ttgcgaagcc gttaccccga ttcgccgtac cgttactatt gaagatgtgg
    28381 gtaactccgc ggcattcctg tgctccgatc tctctgccgg tatctccggt gaagtggtcc
    28441 acgttgacgg cggtttcagc atcgctgcaa tgaacgaact cgaactgaaa taatcgttct
    28501 gttggtcaag acgggcggcg ttctgccgcc cgttatctct gttatacctt tctgatattt
    28561 gttatcgccg atccgtcttt ctccccttcc cgccttgcgt caggataacg atttccttta
    28621 cgaccaagga gcgcccatgg aaaaacgcca cattaccggc aaaagctact ggtatcatga
    28681 aacgcaatcc agtactgcgg agtatgacgt tctgcctctg gtcccggaag ccgcaaaggt
    28741 cagcgatccc tttctgctcg acgtgatcct tgatgaagaa acgctggccc ccttcctttc
    28801 atggctggtc cctgcgcgcg ttcttgcagt ggaattgttc cctgaccagc ttaccgtgac
    28861 ccgttcacag accttcaccg cttatgaacg cttgtcgacg gccctgacgg ttgctcaggt
    28921 ttgcggcgtc caacggttat gtaactacta ttcggcgcga cttacgccgc tccccgggcc
    28981 tgattcctcc agggaaagta atcatcggtt agcacaaatc acgcaatatg cccgccaact
    29041 ggctagctcg ccttctatta tcgacaaccg atcgcgccag catctgaatg acgtcggcct
    29101 aactgcctgg gactgtgtaa tcattaacca aatcattggt tttattggct ttcaggcgcg
    29161 gtcaattgcg acatttcagg cttatctcgg gcatccggta cgctggttac ccggtctgga
    29221 gatacaaaac tatgccgacg cgtcactgtt tgctgatgaa tcaatacgct ggcgaagcag
    29281 ctatgaggtg gaaaaactcc ctgaagagca cacaaaaagt tcaactgcag aactttgcca
    29341 actggccgaa acactctctc tccaccctat ttcactttcc cttctcgaaa agttgctaaa
    29401 cagcacacgg gtcaatacac agccggataa tcagcttgcg gcgttgttat gcgcgcggat
    29461 aaatggcagt cctgcttgtt ttgccacctg tatgaattca tcaaatgaat ataaaaaaat
    29521 cagcgccctt ctgcgcaagg gcgaaaatga aattaaccaa tgggctgacc gtcattctgt
    29581 tgagcgcgct accgttcagg cgatacaatg gctgacccga gcacccgatc gctttagcgc
    29641 cgcccagttc agcccattac tcgaacacga aaaatcatca acgcagatta ttaatctgct
    29701 ggtatggagc gggctgtgtg gctggataaa tcgcttaaaa atcgcgttgg gtgagacata
    29761 ttaaccttgc cgcgtcagac agattcgcgt aaaactgtca gccgctctaa tggccaccaa
    29821 aatagacaat tatgtttcag gacaacccgc tgctagcgca gcttaaacag caactgcatt
    29881 cccagacgcc acgcgctgaa ggggtggtaa aagccacaga aaaaggcttt ggcttcctgg
    29941 aagtcgacgc gcaaaaaagt tatttcattc cgccgccgca gatgaaaaaa gtcatgcatg
    30001 gcgaccgaat tatcgcggtt atccacagtg aaaaagaacg tgaatccgca gagccagaag
    30061 aactggttga accgtttctg actcgtttcg tgggtaaagt tcagggcaaa aatgaccgtc
    30121 tggccatcgt tcctgatcat ccactcttaa aagacgccat tccttgccgc gcagcccgtg
    30181 gcctgaacca cgagtttaaa gaaggcgact gggcggttgc cgaaatgcgc cgtcatccgc
    30241 tgaaaggcga tcgttctttc tatgctgaac tgacacaata catcactttt ggtgacgacc
    30301 actttgtacc gtggtgggtt acccttgcgc gccataatct ggagaaagaa gcaccagacg
    30361 gcgtcgctac cgaaatgctc gatgaaggtc tggttcgtga agatctgacc gcgctggatt
    30421 ttgtcaccat cgacagtgcc agcacagaag atatggatga cgcccttttc gctaaggcgt
    30481 tgccggatga caaacttcag ctgattgtgg cgattgccga tccaaccgcg tggattgctg
    30541 aaggcagcaa gctggacaaa gccgcgaaaa ttcgcgcatt caccaactat ctgcctggct
    30601 tcaacatccc tatgctgcct cgcgagcttt ctgacgatct ctgctcactg cgcgccaatg
    30661 aagtccgccc ggtactggca tgccgcatga cgctctccgc tgatggcacc attgaagata
    30721 atatcgaatt ctttgccgcc accatcgaat ccaaagcgaa gctggtgtat gacctggttt
    30781 ctgactggct ggaaaatacc ggtgactgga agcctgaaag tgaagcaatt gctgaacaag
    30841 tccgtttgtt agcgcaaatt tgccaacgcc gcggcgagtg gcgtcataac cacgcactgg
    30901 tgtttaaaga tcgcccggat taccgcttta ttctcggtga aaaaggtgaa gtgctggata
    30961 tcgtcgccga gcctcgtcgc attgccaacc gtatcgtcga agaagcgatg attgccgcta
    31021 acatttgtgc ggcccgcgta ctgcgcgata agctcggttt tggcatctat aacgtgcata
    31081 tgggctttga tccggcgaat gccgacgcgt tggcagcgtt gctgaaaacg cacggtctgc
    31141 atgtcgatgc tgaagaagtg ctcacgctgg acggtttctg caaactgcgt cgtgaactgg
    31201 atgcgcaacc aactggtttc ctcgacagcc gcattcgtcg cttccagtca tttgctgaaa
    31261 ttagcactga acccggtcct cactttggcc tcggtctgga ggcatacgcc acctggactt
    31321 cgccgatccg taaatatggc gacatgatca accaccgtct gctgaaagcg gttatcaaag
    31381 gcgaaactgc gacgcgtccg caggatgaaa tcactgtcca aatggccgag cgtcgccgtc
    31441 tcaaccgaat ggcagaacgt gatgttggcg actggttata cgcacgcttc ctgaaagaca
    31501 aagccgggac cgacacccgt ttcgcggcgg aaattgtcga tatcagccgt ggcggcatgc
    31561 gtgtgcgttt ggttgataac ggcgctatcg cctttattcc ggcacctttc ttacacgctg
    31621 tgcgcgatga actggtttgc agccaggaaa acggcactgt acaaattaaa ggtgaaacgg
    31681 cttacaaagt gactgacgtt attgacgtca ccattgccga agtccgcatg gaaacccgca
    31741 gcattattgc gcgcccggtc gcgtaatctc ctttcacggc ccattcctta tgaatgggcc
    31801 gtttatttcc ccgctctacc ttcatcatat cccggcagtt ttttaactga ctttcgtttg
    31861 aaaactggcg tttttccaaa actggattac aaataaatat ataaaaaaaa caatgcgttt
    31921 agccgccgtt aatttcatgt aagtcatgtt gttcctttta tttaaccgcg gactccgctg
    31981 ttaaccggag gatatgcatc atgaaaaccg ttagggagtc cacaacgttg tacaactttc
    32041 tcggatcgca caatccatac tggcggttaa cggaaagcag cgatgttttg cgcttttcta
    32101 ccaccgaaac cacagaacct gaacgtacgt tgcagttatc tgccgaacag gctgctcgca
    32161 tcagggaaat gacggtcatc acctccagcc tgatgatgag tctgaccgtc gatgaaagcg
    32221 atctttctgt gcatctggta ggacgaaaaa tcaataaacg ggaatgggct ggcaacgcgt
    32281 ctgcatggca tgacacacca gcagttgctc gtgatttatc acacggactt tcctttgctg
    32341 agcaggtagt ttctgaagca cattccgcaa tagtgattct cgacagccgg gggaatatcc
    32401 aacgcttcaa tcggttatgt gaagattaca cagggttgaa agaacatgac gtcattgggc
    32461 aaagcgtgtt taaactgttt atgagccgtc gtgaagctgc ggcatccagg cgcaataacc
    32521 gtgtattttt tcgaagcggc aatgcatatg aagtcgaact gtggatacca acacgtaaag
    32581 gccagcggct gtttctgttt cgcaataaat ttgtccacag cggcagtggc aagaacgaaa
    32641 tttttttaat ctgttccggc accgacatta ccgaagagcg ccgcgctcag gagcgactgc
    32701 gtattctggc aaataccgac agtatcaccg gactgccgaa tcgtaacgca atgcaggagt
    32761 taatcgatca cgccattaat caggcagata acaataaagt tggggttgtg tatcttgatt
    32821 tggataattt taaaaaggtc aacgacgctt atgggcattt gtttggtgac cagctattac
    32881 gcgacgtgtc attggccatt ttaagctgtc tcgaacatga tcaggtgttg gcgcgtccag
    32941 gtggcgatga gtttctggta ctggcatcca atacctcaca aagcgcgctg gaagcaatgg
    33001 catcacgaat tttgacccgc ttacgactcc ccttccgcat tggtttaatt gaagtttata
    33061 ccagctgttc agtgggtatt tcactctctc ccgaacatgg ttcggacagc gcggctatta
    33121 ttcgtcacgc cgacacagca atgtacacag cgaaggaagg cggacgagga caattttgcg
    33181 tttttactcc agaaatgaat cagcgggtat ttgaatatct ctggctggat accaacttgc
    33241 gtaaagcact ggaaaacgat cagttggtta ttcactatca accgaaaatc acctggcgtg
    33301 gcgaagtgcg cagtctggaa gcactagtac gttggcagtc acctgaacgt ggattgattc
    33361 caccgttgga cttcatttcc tacgccgaag agtcagggct aattgtgcct ttaggccgtt
    33421 gggtgattct cgatgtagta cgccaggtgg caaagtggcg ggataaaggt attaacctgc
    33481 gagtggcggt aaacatttct gcacgtcagc tcgccgatca aaccattttc accgccctga
    33541 aacaggttct ccaggaactc aattttgaat actgccctat tgatgttgaa ctgacggaga
    33601 gctgcctgat tgagaatgat gagctggcac tgtctgttat tcaacaattt agccaactag
    33661 gtgcgcaagt gcatctggac gattttggta ccggttactc ttcactttcg caactggcgc
    33721 gctttccgat cgatgccatc aaacttgacc aggtttttgt tcgagatatt cacaaacaac
    33781 ctgtctcgca gtcactggtc cgggcgatcg tcgctgtggc ccaggcattg aatcttcagg
    33841 tgatcgccga aggtgtagag agtgcaaagg aagatgcttt tttaaccaag aacgggatca
    33901 atgagcggca aggatttttg tttgccaaac cgatgcccgc cgtcgccttc gaacgttggt
    33961 ataaacgcta tctgaag
//
LOCUS       DAZFLD010000020        33702 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.020, whole genome shotgun sequence.
ACCESSION   DAZFLD010000020 DAZFLD010000000
VERSION     DAZFLD010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33702
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.020"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            113..673
                     /locus_tag="ACHKBL_000886"
     CDS             113..673
                     /locus_tag="ACHKBL_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000657001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5817799.1"
                     /translation="MKEEPEIIEINSEEFTENEWLTETDPRVLALTMEGYIVFPPFFS
                     DAEYSAINNAGIDRAFHKYELDCGPVYRVCRHGQVKKCFTRKTAIIHLAHFMTTKVFE
                     SSGFEKRLPDQCLMHPEYGEVFHPGEITPEYYRAHGRCFHRLLRILARRKQFDAWMEK
                     YNAWIDQYCRLVAQRPFKKRANHDCN"
     gene            660..1244
                     /locus_tag="ACHKBL_000887"
     CDS             660..1244
                     /locus_tag="ACHKBL_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000127373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5817800.1"
                     /translation="MTATEQLFFLTAEGIHTPYGLLDINKALEMLDEGCFDEPIWHGL
                     EIVCCISEAEEKGMTGELAPEKTIMYWRWLYTAMFFSEQLTRNGWTEVLNENGGTDKG
                     TMYRGHNGGMTIYLKPLRLAMQNYMEWALIETYGAESGLLKSILMYKAFTGALGDGEK
                     TGITMEGYRAMHMILDGLISEATDNSEPETRVFH"
     gene            1268..2134
                     /locus_tag="ACHKBL_000888"
     CDS             1268..2134
                     /locus_tag="ACHKBL_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HFO5817801.1"
                     /translation="MKKSTELVPVAARDLQIIEYRGQRVVTTEQLAAGYGATPIRIRQ
                     NHSRNEERFVVGKHYFLLEGDELRELKHRVSQSYSVKIASNVRSLILWTERGAANHAK
                     LLETDQAWNYHEDLVEFYFTQRENIDVQRVLTRKELALMVIEAEERAEAMALENKTLS
                     ATVDSLEKHFSKGITVPQFCRALNGVNTSQIMWWLSGRKWVFNEQRDPEKTPRWRTAS
                     YARDRYLTEEAVQITPHGKATFTKFTPVLLEKGCHRLYQFYMNGELPMKKNWNGEFKH
                     DKAIYTPEAEHE"
     gene            2127..2489
                     /locus_tag="ACHKBL_000889"
     CDS             2127..2489
                     /locus_tag="ACHKBL_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 family protein"
                     /protein_id="HFO5817802.1"
                     /translation="MNSTTAAIMNYDPNMTLCGRMARQTVRLTLGQWEYRETFEVDVV
                     GNLTGLDVITCAIENLYEDLPYEEAQDEYTGETNIMATIQIGDLACTDEDMLGEAWLA
                     GMLIAAEIISIEPAGTLS"
     gene            2506..2730
                     /locus_tag="ACHKBL_000890"
     CDS             2506..2730
                     /locus_tag="ACHKBL_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5817803.1"
                     /translation="MIISFTDYGEQAKLIITSSRLTLRKHNRVVDAVLLCTPDITESR
                     SGFFLVKSSLSGKTRDILRAYKTATRESER"
     gene            2727..3629
                     /locus_tag="ACHKBL_000891"
     CDS             2727..3629
                     /locus_tag="ACHKBL_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5817804.1"
                     /translation="MKEADLKALRDAAQNTVYGDKGCDLEAFHSLATPPVILALISHI
                     ENLEKKLDEIVTSEISPKAWLNNLTGEVCSIDAMPDARNDRETYTPLYPAWQRIAAAD
                     DNGRVAWMYTHKKAGYRVPTMTSWESLAEERGGSLDKYIVSPLFTVPQIPPVRTEQDA
                     YPDVQVSWDATRHYVNGWNACRQAMLTTAEKVLDVRIPEYIGRWLSAALHDQNSCEEM
                     KNDIRRFLAEYHHVPSKYNGWRLVPDKLTEQMKYRIVPLLATIKPRSVDEYDRSGENA
                     QFLEWVWTQLMEATKFAYEGSVNG"
     gene            3622..3864
                     /locus_tag="ACHKBL_000892"
     CDS             3622..3864
                     /locus_tag="ACHKBL_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000504369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5817805.1"
                     /translation="MGEMVDINDELVRTVITVDDGCDHTQRFIWMMNTRRNIRNKVFR
                     HEPKPVRVTKIRIEARKRRKYRPRKARAPAQPNELA"
     gene            3885..4031
                     /locus_tag="ACHKBL_000893"
     CDS             3885..4031
                     /locus_tag="ACHKBL_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5817806.1"
                     /translation="MNREFEIWIRLRYGYRYDLTRDAHGFYCREVVKRMFDVWCHCRG
                     LKIV"
     gene            4039..4293
                     /gene="xisR"
                     /locus_tag="ACHKBL_000894"
     CDS             4039..4293
                     /gene="xisR"
                     /locus_tag="ACHKBL_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family protein"
                     /protein_id="HFO5817807.1"
                     /translation="METIIYQITPSKWCTERTLIAATGLKSGTIARARKKSWLLGKEY
                     RHYAAEGEPGPFSECVYNIEEIMRWIENQKQPGAKNASSG"
     gene            4327..5616
                     /locus_tag="ACHKBL_000895"
     CDS             4327..5616
                     /locus_tag="ACHKBL_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO5817808.1"
                     /translation="MSNASYPTGVENHGGSLRIWFHYNGKRVRENLGVPDTAKNRKIA
                     GELRTSVCFAIRMGSFDYAAQFPNSPNLKHFGLGKKEITIKALSEKWLELKKMEITAN
                     AMVRYKSAIRNCLDCLGGNKLASSITQEDVLIFRKELLTGYQLPGKNQRKPIKGRSVP
                     TVNSYMTIISGMFRFAANNGYIGKNPFSELPSLKRAKTPPDPLTKDEFARLIDACHHQ
                     QTKNFWSLAVYTGMRHGELCGLAWEDIDLEAGTLTVKRNYTQTKEFTLPKTDAGTNRV
                     IQLIQPAIDVLKNQATLTRLGKQYQIEVKLREYGRTSTHSCSFVFNPQILDRTGNSGV
                     HYAVTSINRTWESALKRAKIRHRKAYQSRHTYACWALTAGANPNFIANQMGHANAQMV
                     YNVYGAWMKDNNQSQVEVLNRKLCTYVPQVPQRLNKA"
     gene            5661..6311
                     /gene="rcsB"
                     /locus_tag="ACHKBL_000896"
     CDS             5661..6311
                     /gene="rcsB"
                     /locus_tag="ACHKBL_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor RcsB"
                     /protein_id="HFO5817809.1"
                     /translation="MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINN
                     LPKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLD
                     IEGIVLKQGAPTDLPKALAALQKGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLR
                     LFAEGFLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVTLSPADKD"
     gene            complement(6397..9246)
                     /gene="rcsC"
                     /locus_tag="ACHKBL_000897"
     CDS             complement(6397..9246)
                     /gene="rcsC"
                     /locus_tag="ACHKBL_000897"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0018106 - peptidyl-histidine
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RcsC"
                     /protein_id="HFO5817810.1"
                     /translation="MKYLASFRTTLKASRYMFRALALVLWLLIAFSSVFYIVNALHQR
                     ESEIRQEFNLSSDQAQRFIQRTSDVMKELKYIAENRLSAENGVLSPRGRETQTDVPAF
                     EPLFADSDCSAMSNTWRGSLESLAWFMRYWRDNFSAAYDLNRVFLIGSDNLCMANFGL
                     RDMPVERDTALKALHERINKYRNAPQDDSGSNLYWISEGPRPGVGYFYALTPVYLANR
                     LQALLGVEQTIRMENFFLPGTLPMGVTILDENGHTLISLTGPESKIKGDPRWMQERSW
                     FGYTEGFRELVLKKNLPPSSLSIVYSVPVDKVLERIRMLILNAILLNVLAGAALFTLA
                     RMYERRIFIPAESDALRLEEHEQFNRKIVASAPVGICILRTADGVNILSNELAHTYLN
                     MLTHEDRQRLTQIICGQQVNFVDVLTSNNTNLQISFVHSRYRNENVAICVLVDVSSRV
                     KMEESLQEMAQAAEQASQSKSMFLATVSHELRTPLYGIIGNLDLLQTKELPKGVDRLV
                     TAMNNSSSLLLKIISDILDFSKIESEQLKIEPREFSPREVMNHITANYLPLVVRKQLG
                     LYCFIEPDVPVALNGDPMRLQQVISNLLSNAIKFTDTGCIVLHVRADGDYLSIRVRDT
                     GVGIPAKEVVRLFDPFFQVGTGVQRNFQGTGLGLAICEKLISMMDGDISVDSEPGMGS
                     QFTVRIPLYGAQYPQKKGVEGLSGKRCWLAVRNASLCQFLETSLQRSGIVVTTYEGQE
                     PTPEDVLITDEVVSKKWQGRAVVTFCRRHIGIPLEKAPGEWVHSVAAPHELPALLARI
                     YLIEMESDDPANALPLTDKAVSDNDDMMILVVDDHPINRRLLADQLGSLGYQCKTAND
                     GVDALNVLSKNHIDIVLSDVNMPNMDGYRLTQRIRQLGLTLPVIGVTANALAEEKQRC
                     LESGMDSCLSKPVTLDVIKQTLTVYAERVRKSRES"
     gene            9413..11239
                     /gene="atoS"
                     /locus_tag="ACHKBL_000898"
     CDS             9413..11239
                     /gene="atoS"
                     /locus_tag="ACHKBL_000898"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     AtoS"
                     /protein_id="HFO5817811.1"
                     /translation="MHYMKWIYPRRLRNQMILMAILMVIVPTLTIGYIVETEGRSAVL
                     SEKEKKLSAVVNLLNQALGNRYDLYIDLPREERIRALNAELAPITENITHAFPGIGAG
                     YYNKTLDAIITYAPSALYQNNVGVTIAADHPGREVMRTNTPLVYSGRQVRGDILNSMI
                     PIERNGEILGYIWANELTEDIRRQAWKMDVRIIIVLTAGLLISLLLIVLFSRRLSANI
                     DIITDGLSTLAQNIPTRLPQLPGEMGQISQSVNNLAQALRETRTLNDLIIENAADGVI
                     AIDRQGDVTTMNPAAEVITGYQRHELVGQPYSMLFDNTQFYSPVLDTLEHGTEHVALE
                     ISFPGRDRTIELSVTTSRIHNTHGEMIGALVIFSDLTARKETQRRMAQAERLATLGEL
                     MAGVAHEVRNPLTAIRGYVQILRQQTRDPIHQEYLSVVLKEIDSINKVIQQLLEFSRP
                     RHSQWQQVSLNALVEETLVLVQTAGVQARVDFISELDNELSPINADRELLKQVLLNIL
                     INAVQAISARGKIRIRTWQYSDSQQAISIEDNGCGIDLSLQKKIFDPFFTTKASGTGL
                     GLALSQRIINAHQGDIRVASLPGYGATFTLILPINPQGNQTV"
     gene            11236..12621
                     /gene="atoC"
                     /locus_tag="ACHKBL_000899"
     CDS             11236..12621
                     /gene="atoC"
                     /locus_tag="ACHKBL_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate metabolism transcriptional
                     regulator AtoC"
                     /protein_id="HFO5817812.1"
                     /translation="MTAINRILIVDDEDNVRRMLSTAFALQGFETHCANNGRTALHLF
                     ADIHPDVVLMDIRMPEMDGIKALKEMRSNETRTPVILMTAYAEVETAVEALRCGAFDY
                     VIKPFDLDELNLIVQRALQLQSMKKEIRHLHQALSTSWQWGHILTNSPAMMDICKDTA
                     KIALSQASVLISGESGTGKELIARAIHYNSRRAKGPFIKVNCAALPESLLESELFGHE
                     KGAFTGAQTLRQGLFERANEGTLLLDEIGEMPLVLQAKLLRILQEREFERIGGHQTIK
                     VDIRIIAATNRDLQAMVKEGTFREDLFYRLNVIHLILPPLRDRREDISLLANHFLQKF
                     SSENQRDIIDIDPMAMSLLTAWSWPGNIRELSNVIERAVVMNSGPIIFSEDLPPQIRQ
                     PVCNAGEAKTAPVGERNLKEEIKRVEKRIIMEVLEQQEGNRTRTALMLGISRRALMYK
                     LQEYGIDPADV"
     gene            12817..13479
                     /gene="atoD"
                     /locus_tag="ACHKBL_000900"
     CDS             12817..13479
                     /gene="atoD"
                     /locus_tag="ACHKBL_000900"
                     /EC_number="2.8.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate CoA-transferase subunit alpha"
                     /protein_id="HFO5817813.1"
                     /translation="MKTKLMTLQDATGFFRDGMTIMVGGFMGIGTPSRLVEALLESGV
                     RDLTLIANDTAFVDTGIGPLIVNGRVRKVIASHIGTNPETGRRMISGEMDVVLVPQGT
                     LIEQIRCGGAGLGGFLTPTGVGTVVEEGKQTLTLDGKTWLLERPLRADLALIRAHRCD
                     TLGNLTYQLSARNFNPLIALAADITLVEPDELVETGELQPDHIVTPGAVIDHIIVSQE
                     SK"
     gene            13479..14129
                     /gene="atoA"
                     /locus_tag="ACHKBL_000901"
     CDS             13479..14129
                     /gene="atoA"
                     /locus_tag="ACHKBL_000901"
                     /EC_number="2.8.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate CoA-transferase subunit beta"
                     /protein_id="HFO5817814.1"
                     /translation="MDAKQRIARRVAQELRDGDIVNLGIGLPTMVANYLPEGIHITLQ
                     SENGFLGLGPVTTAHPDLVNAGGQPCGILPGAAMFDSAMSFALIRGGHIDACVLGGLQ
                     VDEEANLANWVVPGKMVPGMGGAMDLVTGSRKVIIAMEHCAKDGSAKILRRCTMPLTA
                     QHAVHMLVTELAVFRFIDGKMWLTEIADGCDLATVRAKTEARFEVAADLNTQRGDL"
     gene            14126..15448
                     /gene="atoE"
                     /locus_tag="ACHKBL_000902"
     CDS             14126..15448
                     /gene="atoE"
                     /locus_tag="ACHKBL_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00366 family protein"
                     /protein_id="HFO5817815.1"
                     /translation="MIGRISRFMTRFVSRWLPDPLIFAMLLTLLTFVIALWLTPQTPI
                     SMVKMWGDGFWNLLAFGMQMALIIVTGHALASSAPVKSLLRTAASAAKTPVQGVMLVT
                     FFGSVACVINWGFGLVVGAMFAREVARRVPGSDYPLLIACAYIGFLTWGGGFSGSMPL
                     LAATPGNPVEHIAGLIPVGDTLFSGFNIFITVALIVVMPFITRMMMPKPSDVVSIDPK
                     LLMEEADFQKQLPKDAPPSERLEESRILTLIIGALGIAYLAMYFSEHGFNITINTVNL
                     MFMIAGLLLHKTPMAYMRAISAAARSTAGILVQFPFYAGIQLMMEHSGLGGLITEFFI
                     NVANKDTFPIMTFFSSALINFAVPSGGGHWVIQGPFVMPAAQALGADLGKSVMAIAYG
                     EQWMNMAQPFWALPALAIAGLGVRDIMGYCITALLFSGVIFVIGLTLF"
     gene            15479..16663
                     /gene="atoB"
                     /locus_tag="ACHKBL_000903"
     CDS             15479..16663
                     /gene="atoB"
                     /locus_tag="ACHKBL_000903"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA acetyltransferase"
                     /protein_id="HFO5817816.1"
                     /translation="MKNCVIVSAVRTAIGSFNGSLASTSAIDLGATVIKAAIERAKID
                     SLHVDEVIMGNVLQAGLGQNPARQALLKSGLAETVCGFTVNKVCGSGLKSVALAAQAI
                     QAGQAQSIVAGGMENMSLAPYLLDAKARSGYRLGDGQVYDVILRDGLMCATHGYHMGI
                     TAENVAKEYGITREMQDELALHSQRKAAAAIESGAFTAEIVPVNVVTRKKTFVFSQDE
                     FPKADSTTEALGALRPAFDKAGTVTAGNASGINDGAAALVIMEESAALAAGLTPLARI
                     KSYASGGVPPALMGMGPVPATKKALQLAGLQLADIDLIEANEAFAAQFLAVGKTLGFD
                     SEKVNVNGGAIALGHPIGASGARILVTLLHAMQARDKTLGLATLCIGGGQGIAMVIER
                     LN"
     gene            complement(16737..17513)
                     /gene="yfaP"
                     /locus_tag="ACHKBL_000904"
     CDS             complement(16737..17513)
                     /gene="yfaP"
                     /locus_tag="ACHKBL_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaP family protein"
                     /protein_id="HFO5817817.1"
                     /translation="MRKIFLPLLLVALSPVAHSEGVQEVEIDAPLSGWHPAEGEDASF
                     SQSINYPASSVNMADDQNISAQIRGKIKNYAAAGKVQQGRLVVNGASMPQRIESDGSF
                     ARPYIFTEGSNSVQVISPDGQSRQKMQFYSTPGTGTIRARLRLVLSWDTDNTDLDLHV
                     VTPDGEHAWYGNTVLKNSGALDMDVTTGYGPEIFAMPAPIHGRYQVYINYYGGRSETE
                     LTTAQLTLITDEGSVNEKQETFIVPMRNAGELTLVKSFDW"
     gene            complement(17518..19167)
                     /gene="yfaQ"
                     /locus_tag="ACHKBL_000905"
     CDS             complement(17518..19167)
                     /gene="yfaQ"
                     /locus_tag="ACHKBL_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaQ family protein"
                     /protein_id="HFO5817818.1"
                     /translation="MNWRRIVWLLALVTLPTLAEETPLQLVLRGAQHDQLYQLSSSGV
                     TKVSALPDTLTTPLGSLWKLYVYAWLEDTHQPEQPYQCRGNSPEEVYCCQAGESITRD
                     TALVRSCGLYFAPQRLHIGADVWGQYWQQRQAPAWLASLTTLKPETSVTVNSLLDSLA
                     TLPAQNKAQEVLLDVVLDEAKIGVASMLGSRVRVKTWSWFADDKQEIRQGGFAGWLTD
                     GTPLWVTGSGTSKTVLTRYATVLNRVLPVPTQVASGQCVLVELFARYPLKKITAEKSS
                     TAVKPGVLNGRYRVTFANGNHIMFVSHGETTLLSEKGKLKLQSHLDREEYVARVLDRE
                     AKSTPPEAAKAMTVAIRTFLQQNANREGDCLTIPDSSATQRVSASPATTGARTMTAWT
                     QDLIYAGDPVHYHGSRATEGTLSWRQATAQAGQGERYDQILAFAYPDNSLSRWGAPRS
                     TCQLLPKAKAWLAKKMPQWRRILQGETGYNEPDVFAVCRLVSGFPYTDRQQKRLFIRN
                     FFTFQDRLDLTHEYLHLAFDGYPTGLDENYIETLTRQLLMD"
     gene            complement(19168..23682)
                     /locus_tag="ACHKBL_000906"
     CDS             complement(19168..23682)
                     /locus_tag="ACHKBL_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0010951 - negative regulation of
                     endopeptidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin"
                     /protein_id="HFO5817819.1"
                     /translation="MGTGLANADDSLPSSNYAPPAGGTFFLLADSSFSSSEEAKVRLE
                     APGRDYRRYQMEEYGGVDVRLYRIPDPMAFLRQQKNLHRIVVQPQYLGDGLNNTLTWL
                     WDNWYGKSRRVMQRTFSSQSRQNVTQALPELQLGNAIIKPSRYVQNNQFSPLKKYPLV
                     EQFRYPLWQAKPVEPQQGVKLEGASSNFISPQPGNIYIPLGKQEPGLYLVEAMVGGYR
                     ATTVVFVSDTVALSKVSGNELLVWTAGKKQGEAKPGSEILWTDGLGVMTRGVTDDSGT
                     LQLQHISPERSYILGKDAEGGVFVSENFFYESEIYNTRLYIFTDRPLYRAGDRVDVKV
                     MGREFHDPLHSSPIVSAPAKLSVLDANGSLLQTVNVTLDARNGGQGSFRLPENAVAGG
                     YELRLAYRKQVYSSSFRVANYIKPHFEIGLALDKKEFKTGEAVSGKLQLLYPDGEPVK
                     DARVQLSLRAQQLSMVGNDLRYAGRFPVSLEGSETVSDDSGHVALNLPAADKPSRYLL
                     TVSASDGAAYRVTTTKEILIERGLAHYSLSTAAQYSNSGESVVFRYAALESSKQVPVT
                     YEWLRLEDRTSHSGDLPSGGKSFTVNFDKPGNYNLTLRDEDGLILAGLSHAVSGKGST
                     AHTGTVDIVADKTLYQPGETAKMLITFPEPIDEALLTLERDRVEQQSLLSHPANWLTL
                     QRLNDTQYEARIPVSNSFAPNITFSVLYTRNGQYSFQNAGIKVAVPQLDIRVKTEKTH
                     YQPGELVNVELTSSLKGKPVSAQLTVGVVDEMIYALQPEIAPNIGKFFYPLGRNNVRT
                     SSSLSFISYDQALSSEPVAPGATNRSERRVKMLERPRREEVDTAAWMPSLTTDKQGKA
                     YFTFLMPDSLTRWRITARGMNGDGLVGQGRAYLRSEKNLYMKWSMPTVYRMGDKPAAG
                     LFIFSQQDNEPVALVTKFAGAEMRQTLTLHKGANYISLTQNIQQSGLLSAELQQNGQV
                     QDSISTKLSFVDNSWPVEQQKNVMLGGGDNALMLPEQASNIQLQSSETPQEIFRNNLD
                     ALVDEPWGGVINTGSRLIPLSLAWRSLADHQSAAANDIRQMIQDNRLRLMQLAGPGAR
                     FTWWGEDGNGDAFLTAWAWYADWQASQALGVTQQPDYWQHMLDSYAEQADNMPILHRA
                     LVLAWAQEMNLPCKTLLKGLDEAIARRGTKTEDFSEEDTSDINDSLILDTPESPLADA
                     VANVLTMTLLKKAQLKSTVMPQVQQYAWDKAANSNQPLAHTVVLLNSGGDATQAAAIL
                     SGLTAEQSTIERALAMNWLAKYMATMPPVVLPAPAGAWAKHKLTGGGEDWRWVGQGVP
                     DILSFGDELSPQNVQVRWREPAKTAQQSNIPVTVERQLYRLIPGEEEMSFTLQPVTSN
                     EIDSDALYLDEITLTSEQDAVLRYGQVEVPLPPGADVERTTWGISVNKPNAGKQQGQL
                     LEKARNEMGKLAYMVPVKELTGTVTFRHLLRFSQKGQFVLPPARYVRSYAPAQQSVAA
                     GSEWTGMQVK"
     gene            complement(23706..24329)
                     /gene="yfaT"
                     /locus_tag="ACHKBL_000907"
     CDS             complement(23706..24329)
                     /gene="yfaT"
                     /locus_tag="ACHKBL_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 domain-containing protein"
                     /protein_id="HFO5817820.1"
                     /translation="MRHGLLALICWLCCVVTHSEMLNVEQSGLFRAWFVRIAQEQLRQ
                     GPSPRWYQQDCAGLVRFAANETLKVHDSKWLKSNGLSNQYLPPEMTLTPEQRQLAQNW
                     NQGNGKTGPYVTAINLIQYNSQFIGQDINQALPGDMIFFDQGDAQHLMVWMGRYVIYH
                     TGSATKTDNGMRAVSLQQLMTWKDTRWIPNDSNPNFIGIYRLNFLAR"
     gene            complement(24326..26014)
                     /gene="yfaA"
                     /locus_tag="ACHKBL_000908"
     CDS             complement(24326..26014)
                     /gene="yfaA"
                     /locus_tag="ACHKBL_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416733.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2138 domain-containing protein"
                     /protein_id="HFO5817821.1"
                     /translation="MSGEKKAKGWRFYGLVGLGAIVLLSAGVWALQYAGSGPEKTLSP
                     LVVHNNLQIDLNEPDLFLDSDSLSQLPKDLLTIPFLHDVLSEDFVFYYQNHADRLGIE
                     GSIRRIVYEHDLTLKDKLFSSLLDQPAQAALWHDKQGHLSHYMVLIQRSGLSKLLEPL
                     LFAATSDSQLSKTEISSIKLNSETIPVYQLRYNGNNALMFATYQDKMLVFSSTDMLFK
                     DDQQDTEATAIASDLLSGKKRWQASFGLEERAAEKTPVRQRIVVSARLLGFGYQRLMP
                     SFAGVRFEMSNDGWHSFVALNDESASVDASFDFTPVWNSMPAGASFCVAVPYSHGIAE
                     EMLSHISQENDKLNGALDGAAGLCWYEDSKLQTPLFVGQFDGSAEQAQLPGKLFTQNI
                     GAHESKAPEGVLPVNQTQQGEAQIWRREVSSRYGQYPKAQAVQPDQLMSDYFFRVSLA
                     MQNKTMLFSLDDTLVNNALQTLNKTRPAMVDVIPTDGIVPLYINPQGVAKLLRNETLT
                     SLPKNLEPVFYNAAQTLLMPKLDALSQQPRYVMKLAQMEPGAAWQWLPITWQPL"
     gene            complement(26163..28790)
                     /gene="gyrA"
                     /locus_tag="ACHKBL_000909"
     CDS             complement(26163..28790)
                     /gene="gyrA"
                     /locus_tag="ACHKBL_000909"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HFO5817822.1"
                     /translation="MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDSAVYDTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMP
                     TKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEGLMEHIPGPDFP
                     TAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRETIIVHEIPYQVNKARLIEK
                     IAELVKEKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINM
                     VALHHGQPKIMNLKDIIAAFVRHRREVVTRRTIFELRKARDRAHILEALAVALANIDP
                     IIELIRHAPTPAEAKTALVANPWQLGNVAAMLERAGDDAARPEWLEPEFGVRDGLYYL
                     TEQQAQAILDLRLQKLTGLEHEKLLDEYKELLDQIAELLRILGSADRLMEVIREELEL
                     VREQFGDKRRTEITANSADINLEDLIIQEDVVVTLSHQGYVKYQPLSEYEAQRRGGKG
                     KSAARIKEEDFIDRLLVANTHDHILCFSSRGRVYSMKVYQLPEATRGARGRPIVNLLP
                     LEQDERITAILPVTEFEEGVKVFMATANGTVKKTVLTEFNRLRTAGKVAIKLVEGDEL
                     IGVDLTSGEDEVMLFSAEGKVVRFKESSVRAMGCNTTGVRGIRLGEGDKVVSLIVPRG
                     DGAILTATQNGYGKRTAVAEYPTKSRATKGVISIKVTERNGLVVGAVQVDDCDQIMMI
                     TDAGTLVRTRVSEISIVGRNTQGVILIRTAEDENVVGLQRVAEPVDEEDLDTIDGSAA
                     EGDDEIAPEVDVDDEPEEE"
     gene            28937..29659
                     /gene="ubiG"
                     /locus_tag="ACHKBL_000910"
     CDS             28937..29659
                     /gene="ubiG"
                     /locus_tag="ACHKBL_000910"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HFO5817823.1"
                     /translation="MNAEKSPVNHNVDHEEIAKFEAVASRWWDLEGEFKPLHRINPLR
                     LGYIAERAGGLFGKKVLDVGCGGGILAESMAREGATVTGLDMGFEPLQVAKLHALESG
                     IQVEYVQETVEEHAAKHAGQYDVVTCMEMLEHVPDPQSVVRACAQLVKPGGDVFFSTL
                     NRNGKSWLMAVVGAEYILRMVPKGTHDVKKFIKPAELLGWVDQTSLKERHMTGLHYNP
                     ITNSFKLGPGVDVNYMLHTQNK"
     gene            complement(29799..33557)
                     /gene="yfaL"
                     /locus_tag="ACHKBL_000911"
     CDS             complement(29799..33557)
                     /gene="yfaL"
                     /locus_tag="ACHKBL_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA-I family autotransporter adhesin YfaL/EhaC"
                     /protein_id="HFO5817824.1"
                     /translation="MRIIFLRKEYLSLLPSMIASLFSGNGAAAVIDSCQGYDIKANCQ
                     ASRQSLSGNMQDWSIADGQWLIFSGMANNASGGAVFLQQSAEFTISPQNETGMTLFAD
                     NSVSGEYNNGGAIFAKENSTINLANVIFDSNVAGGYGGAIYSAGTNDTGAADLRVTNA
                     VFHNNIANDGKGGAIYTINNDVYLSDDAFNNNQAYTSTSYSDGDGGAIDVTDNSTDNT
                     HLSGKTIINNTSFTNNYAEGYGGAIYTSSTTSPYLIDISVDDNYDQNNGVMIDENNSA
                     SGYDHSATAAAGGFMYIGHSVAEFNIAADKTLVIGNTSNDGAIDSLAGTGVIVKEGAG
                     ELVLNADNNAFTGEISIQNGEVTLGRSDELMNVGDTHCQSDPQDCFGLMVGSTVHSEY
                     QAELNVGNTQQTFVHSLTGFANGILNIDAGGNVTVNQGGFSGSIQGEGQLTVAQDGSY
                     LLTGAQSMALTGDIVVEDNAVLSLAGNQADLRAMQSDPQSIVLNGGVLDLSDFTTWDG
                     DSSYNDGLQISGSGGTVIGSNDVVDISSGDDLHIGGSDASQNGVYVVINAGDQRVTLA
                     NNNGYLGNTQIASGTLEVSDNSQLGDTSYNRSVIFTDPQQHSEMDVTTDVDTRSATTG
                     QGRNIEMRADGEIHVEDGVDTQWGGLMADSTGQQLESVSTLTKSGGGTLELTASGTAT
                     SAVRVEDGTLKGEAENIIPYVSSLWVGEDGVFETGQNQDIRSIDATSGGDIDITDGTV
                     LRLTQQDTNQVLDASLFSGDGTLVNATDGVTLTGELNTNLETDSLTYLSDVTVNGNLT
                     NTSGAISLQNGVAGDTLTVNGDYTGGGTLLLDSELNGDDSASDQLVLNGNTAGNTAVV
                     INPITGIGEPTSTGIKVVDFAADPTQFQNNAQFRLAGSGYVNMGAYDYTLVEDNNDWY
                     LRSQEVTPTPPPDPDPTPDPDPTPDPDPTPDPGPTPAYQPVLNAKVGGYFNNLRAANQ
                     AFVMERRDQAGGDGQTLNLRVIGGRYHYTAAGQLAQHEDTSTVQLSGNLFSGHWGDDG
                     EWMLGIVGGYSDNQGDSRSNMTGTRADNQNHGYAVGLTSSWYQHGNQKQGVWLDSWLQ
                     YAWFNNDVSEQDDGEDHYHSSGIIASLEAGYQWLPGHGVVIEPQTQVIYQGVQQDDFT
                     AANHARVSQSQGDDIQTRLGLHSEWSTAVGVTPTLDLNYYHDPHATEIEEDGSTISDD
                     GVKQRGEIKVGVTGNISQRVSLRGSVAWQKGSDDFAQTAGFLSMTVKW"
BASE COUNT         8416 a         8925 c         8243 g         8118 t
ORIGIN      
        1 aaggccaggc tgtatcacag atatatcgca acaaacgaat tgctgagcca catcttctgg
       61 ctgcgctcag agtgaataaa agtaattaac accggatgaa cacggagtaa ccatgaaaga
      121 agaacctgaa atcattgaaa ttaattctga agaattcaca gaaaacgaat ggctgacaga
      181 aaccgatccg cgcgttctgg cgctgacaat ggaaggatac atcgtatttc cacctttctt
      241 cagtgatgcg gaatacagtg caataaataa tgccggaatc gaccgtgctt ttcataaata
      301 cgaactcgat tgcggccctg tttacagagt ctgccgccac ggacaagtta agaaatgctt
      361 cacgcgaaaa acggccatca tacatctcgc tcattttatg acaacgaagg tattcgaaag
      421 ttcaggtttt gaaaaacgtc tgcctgacca gtgcctgatg catccggaat atggagaagt
      481 ctttcatccg ggagaaatta cgcccgaata ctacagagct cacgggcgtt gttttcatcg
      541 cctgttgcgc atccttgcac gcagaaaaca gtttgatgcc tggatggaaa aatacaacgc
      601 ctggattgat cagtattgcc ggcttgtcgc gcaaagacca tttaagaaga gagcaaacca
      661 tgactgcaac tgaacagtta ttttttctta ccgctgaagg tatccacaca ccttacggac
      721 tgcttgatat caacaaagca ctggaaatgc tcgatgaagg ctgttttgac gagccgatct
      781 ggcatggtct ggaaatagtg tgttgcatca gtgaagcaga agaaaaaggc atgacaggtg
      841 agctcgcgcc ggaaaagaca atcatgtact ggcgctggct ttataccgcc atgttcttct
      901 ctgaacagct caccagaaac ggctggaccg aagtgcttaa cgagaatggc ggtacagaca
      961 agggcaccat gtacagaggc cacaacggtg gcatgacaat ctacctgaaa ccattacgtc
     1021 tcgctatgca gaactatatg gaatgggcac tcatagaaac ttacggagcg gaatcagggc
     1081 tgctcaaaag cattcttatg tacaaagcat tcaccggcgc tctgggcgac ggcgaaaaaa
     1141 ccggcatcac aatggaaggt tacagagcga tgcacatgat tctggatggg cttatcagtg
     1201 aagccactga caactcagaa ccggaaacca gagtgttcca ctaatcagct tttcgtgagg
     1261 tgtaatgatg aaaaaatcaa ctgaacttgt gccggtggca gcccgtgatt tgcaaattat
     1321 cgaatatcgc ggtcaacgcg ttgtgaccac tgaacaactg gcggcaggtt atggagcaac
     1381 accaatccgt attcgccaga atcattcccg aaacgaagaa cgattcgttg ttggaaagca
     1441 ttattttttg ctggaaggcg atgaactacg tgaacttaag cacagagtat ctcaaagcta
     1501 ctctgtgaaa attgccagca atgttcgatc cctcatcctc tggaccgaac gcggcgcagc
     1561 caaccacgca aaattgctgg aaacggatca ggcgtggaac tatcacgaag accttgtgga
     1621 gttctacttc actcagcggg aaaacatcga tgttcagcgc gtactcactc gcaaggaact
     1681 cgcgttgatg gttattgagg ccgaagaacg ggctgaagct atggccctgg aaaataaaac
     1741 gctctctgca accgtagaca gtctggaaaa acatttctct aaaggcataa ctgttcccca
     1801 gttctgccgt gcgctgaacg gcgtaaatac cagccagata atgtggtggc tgtctggccg
     1861 aaaatgggta ttcaacgaac aacgcgatcc ggagaaaaca ccccgctggc gcacggcctc
     1921 ttatgcccgt gacagatacc tgaccgaaga agcagtacag atcactccac acgggaaagc
     1981 aacattcacg aaattcaccc ccgtattgct ggaaaaagga tgccaccgtt tataccagtt
     2041 ttacatgaac ggtgaactcc ctatgaagaa aaactggaac ggtgaattta aacacgacaa
     2101 agctatttac acaccggagg cagaacatga atagcactac tgcggcaatc atgaactacg
     2161 acccgaacat gacattatgc gggcgcatgg caagacagac ggttcgtctg acactcgggc
     2221 aatgggagta ccgcgaaaca tttgaagtgg acgttgtcgg caatctgacg ggactggatg
     2281 taataacctg cgcaattgaa aacctttatg aagacctgcc gtatgaagaa gcacaggatg
     2341 aatataccgg tgaaactaac attatggcca caatacagat cggtgatctt gcatgtacgg
     2401 atgaggacat gctcggcgaa gcatggctgg caggtatgct tattgcagca gaaattatca
     2461 gcattgaacc agcaggaaca ctttcataac ggagatcgcc atcagatgat tatttcattc
     2521 accgattacg gcgaacaggc aaaactgatt atcaccagtt cccgcctgac attgcgaaaa
     2581 cataatcgtg tcgttgatgc ggtacttctg tgcacgccgg acatcacaga aagccggagc
     2641 ggctttttcc tggttaagtc gtcattatca ggaaaaacac gcgacatcct gcgcgcctac
     2701 aaaacagcaa caagggagtc tgaacgatga aagaagctga tctgaaagca ctgcgtgacg
     2761 ccgcacaaaa taccgtatat ggcgacaagg gatgtgatct ggaagcattt cactcccttg
     2821 ccactccgcc agttatcctt gcgctaatca gtcacataga aaatctggag aaaaaactgg
     2881 atgaaatcgt aacgtcagag attagcccga aggcctggct gaataacctt acgggggaag
     2941 tttgcagtat tgatgccatg cctgacgccc gtaatgacag agaaacatac accccgcttt
     3001 atcctgcctg gcagcgcatt gccgccgcag atgataacgg cagagtggca tggatgtata
     3061 cgcacaaaaa agcaggttac agggtcccca caatgacatc gtgggaaagt cttgcagaag
     3121 agcggggagg ctctctggat aaatatatcg tcagtccact attcactgtt cctcagatac
     3181 cgccagtgag aacagaacag gatgcttacc ctgatgtgca ggtttcatgg gatgcaacac
     3241 gtcactatgt caacggctgg aacgcctgcc gacaggcaat gctgacgacg gcagaaaaag
     3301 tacttgatgt gcgaatccct gaatatatcg gacgctggct ttccgctgca cttcatgatc
     3361 agaactcatg cgaggaaatg aaaaacgata tacgacgctt cctggctgaa taccaccatg
     3421 ttccatcgaa atataacggc tggcgacttg taccggacaa gttaaccgaa cagatgaagt
     3481 accggattgt gccactactg gcgaccatca agccccgcag cgtcgatgaa tatgaccgct
     3541 caggagaaaa cgcacaattt cttgaatggg tatggacgca actgatggaa gccacgaaat
     3601 tcgcgtatga aggctcagtg aatgggtgaa atggttgata ttaacgatga actggtccgc
     3661 acagtaatta ccgttgatga cggatgcgac catacccagc gttttatctg gatgatgaat
     3721 acacggcgta atatcagaaa caaagtgttc cggcacgaac caaagccagt cagggtgaca
     3781 aagatacgca ttgaagccag aaaacgccgc aaatacaggc cacggaaggc cagggcccca
     3841 gcgcaaccga atgaactggc ataaagacag caggagaata agtcgtgaat cgcgagtttg
     3901 aaatatggat acgactcaga tacgggtacc gatacgattt aaccagagat gctcacggct
     3961 tctactgccg tgaagttgta aagcgaatgt ttgacgtgtg gtgccactgc cgtggcctga
     4021 agatagtgtg agtcaggaat ggaaacaatc atctatcaga taacgcccag caaatggtgc
     4081 acagaaagaa cccttattgc tgcaactgga ctgaaatcag gaaccattgc cagagcaaga
     4141 aaaaaatcct ggttactggg gaaagaatac cgtcattacg cagcagaagg agagcccggg
     4201 cctttcagtg aatgtgtata caacattgag gaaattatgc gctggatcga aaaccagaaa
     4261 caaccaggtg ccaaaaatgc aagttccggt taacctgtta atgctcctgg acgtctggga
     4321 ggtttaatga gtaacgcatc atacccgaca ggcgttgaaa accatggcgg atcactccgc
     4381 atatggtttc actataacgg caaacgtgtc agagaaaacc tcggtgttcc tgacaccgcc
     4441 aaaaaccgga agatcgcagg tgaacttcgc acttccgttt gttttgcaat aagaatgggg
     4501 agcttcgact acgccgcgca gttccctaat tcccctaacc tgaaacattt tggtctggga
     4561 aaaaaagaga taaccattaa ggcactttcg gaaaaatggc ttgagcttaa gaaaatggaa
     4621 ataacagcaa atgccatggt cagatacaaa tcagctatcc gaaattgcct tgactgcctt
     4681 ggagggaaca aactggcatc atcaattaca caggaagatg ttctcatttt tcgtaaagaa
     4741 ttattaacgg gatatcagtt gccaggaaaa aatcagagga agcctattaa aggtcgtagt
     4801 gtacctactg taaatagcta tatgacaatt atctccggga tgttccgttt tgcggcaaac
     4861 aatgggtata taggcaaaaa ccctttcagt gagcttccat cactgaaaag agcgaaaaca
     4921 cctcccgatc cactaacaaa agatgagttt gccagactga ttgatgcgtg ccaccaccag
     4981 caaactaaaa atttctggtc gctggctgta tataccggga tgcggcatgg cgaactgtgt
     5041 gggctggcat gggaggatat cgatctggaa gcgggtacct tgaccgtcaa aaggaattac
     5101 actcagacaa aggaatttac actacccaaa acagatgcag gaacaaacag ggtaatacaa
     5161 ctcatacagc cagcaattga cgtattaaaa aatcaggcga cattaaccag gcttggtaaa
     5221 caatatcaga tagaagtaaa gctgcgagag tatggccgga caagcactca ctcatgcagc
     5281 tttgttttca atcctcagat attagatcgc acaggtaact caggagttca ttatgccgta
     5341 acatccatca acagaacatg ggaatctgca ttaaaacgag caaaaattcg ccatagaaaa
     5401 gcgtatcagt ctcggcatac ttatgcttgc tgggctttaa ctgccggagc aaatccaaac
     5461 ttcatagcca accagatggg acatgcgaat gcccagatgg tttacaatgt ttacggtgca
     5521 tggatgaaag acaataacca gtcccaggta gaagtactaa acagaaagct atgtacttat
     5581 gtcccacagg tgccccaacg actaaacaaa gcatagaata tatttttaaa atcaaatagt
     5641 taatacatag gtgcaagtac atgaacaata tgaacgtaat tattgccgat gaccatccga
     5701 tagtcttgtt cggtattcgc aaatcacttg agcaaattga gtgggtgaat gttgtcggcg
     5761 aatttgaaga ctctacagca ctgatcaaca acctgccgaa actggatgcg catgtgttga
     5821 ttaccgatct ctccatgcct ggcgataagt acggcgatgg cattacctta atcaagtaca
     5881 tcaagcgcca tttcccaagc ctgtcgatca ttgttctgac tatgaacaac aacccggcga
     5941 ttcttagtgc ggtattggat ctggatatcg aagggatcgt gctgaaacaa ggtgcaccga
     6001 ccgatctgcc gaaagctctc gccgcgctgc agaaagggaa gaaatttacc ccggaaagcg
     6061 tttctcgcct gttggaaaaa atcagtgctg gtggttacgg tgacaagcgt ctctcgccaa
     6121 aagagagtga agttctacgc ctgtttgcgg aaggtttcct ggtgaccgag atcgctaaaa
     6181 agctgaaccg cagtatcaaa accatcagta gccagaagaa atctgcgatg atgaagctgg
     6241 gtgtcgagaa cgatattgct ctgctgaatt atctctcttc tgtgacgtta agtccggcag
     6301 ataaagacta atcacctgta ggccagataa gacgcgtcag tgtcttatct ggcatttgca
     6361 cggattgccg gatgcggcgt aaacgcctta tccgtcctac gattcccgcg atttcctgac
     6421 cctctcggca tataccgtca gcgtctgttt tatcacatcc agcgttaccg gcttcgacag
     6481 gcagctgtcc ataccggact ccagacaccg ctgcttctct tcagccaacg cattagcagt
     6541 tactccgatt accggcaacg tcagtcccaa ctgacgaatg cgttgcgtca agcggtaacc
     6601 atccatattt ggcatgttga cgtcgctaag cacgatatca atatgattct tgctaagtac
     6661 attaagcgca tcgacgccat cattcgcggt tttacattga tagcccaacg atcccaactg
     6721 atctgccagc agacgccggt taatcggatg atcatccacg accagaatca tcatatcgtc
     6781 attatcgctg accgctttgt ccgttaacgg cagagcgtta gcaggatcgt cgctctccat
     6841 ctcgatcaaa taaatacgcg ccaacaatgc cggtagctca tgcggggcag ccacactgtg
     6901 tacccactct cctggcgctt tctccagcgg aataccaata tggcgacgac agaaggtcac
     6961 aaccgctcta ccctgccatt ttttgctcac tacctcgtca gtgattaaca catcttcggg
     7021 agtcggttcc tgcccttcgt atgttgtaac gacgatgccg ctgcgctgca aactggtttc
     7081 caggaactga cagagcgacg cattgcggac cgccagccag caacgtttac cactcaaccc
     7141 ttccacgcct tttttctgcg ggtactgagc gccgtacaac ggaatacgca cggtaaactg
     7201 gctgcccatt cccggttctg aatctaccga gatgtcgccg tccatcatgc tgatcagttt
     7261 ttcacaaatc gccagaccca gaccggtccc ctggaagtta cgctgtacgc ccgttccgac
     7321 ctggaagaag ggatcaaaca agcgcaccac ttctttcgcc ggaatcccta cgccggtatc
     7381 gcgaacacgg atagagagat aatcgccatc agcgcgaaca tgcagaacta tacagccggt
     7441 atcggtgaat tttatggcgt tactcaacag gttggagatg acctgctgta aacgcatcgg
     7501 gtcgccattt aaggccactg gcacatccgg ttcaataaag cagtacaagc ctagctgctt
     7561 gcgtaccacc agcggtaaat agttggcggt gatgtggttc atcacttcac gcggtgaaaa
     7621 ctcacgcggt tcgatcttca actgttccga ttcaatcttc gagaagtcga gaatatcgct
     7681 gataattttc aacaacaggc tggaagagtt gttcattgcc gtcaccagac gatcgacgcc
     7741 tttcggtaac tctttggttt gcaacagatc caggttaccg ataatgccat acagcggcgt
     7801 tcgcagctca tgactgacgg tggcaaggaa catcgatttt gactggctcg cctgttccgc
     7861 tgcttgtgcc atctcctgca acgactcttc catcttcacg cgcgaagaaa catccaccag
     7921 cacacaaatg gccacgtttt cattacgata gcgcgaatgg acgaagctga tttgcaggtt
     7981 ggtattgttg ctggtcagga catcaacaaa attgacctgc tgcccacaga taatttgtgt
     8041 cagccgttgg cggtcctcat gcgtaagcat attgagatag gtatgcgcca gttcgttact
     8101 taaaatattg acgccatcag cggtacgcaa aatgcagata cccactggcg cggaggcgac
     8161 aatcttgcga ttaaactgct catgttcttc cagtcgcagg gcgtcgcttt ccgccggaat
     8221 gaaaatacgc cgctcgtaca tccgcgcgag agtaaacaat gcagctccgg caagcacatt
     8281 cagcaaaatt gcgttaagga tcaacatgcg aatgcgttcc agcaccttat caaccggcac
     8341 cgaatacacg atgcttagcg atgagggtgg cagatttttc ttcagcacca gctcccggaa
     8401 cccttccgta tagccaaacc aggagcgttc ctgcatccag cgaggatcgc ccttaatttt
     8461 gctttctggt ccggtaagcg aaatcagggt atgaccattt tcatcaagaa tggtaacccc
     8521 catcggcaac gtaccaggta agaaaaagtt ctccatccgg atggtctgct cgacacccaa
     8581 aagcgcctgc aaccggttcg ccagataaac tggcgtcaag gcgtaaaaat acccgacgcc
     8641 agggcgcgga ccttcgctga tccagtagag gttactgcca ctatcatctt gtggtgcatt
     8701 tcgatattta ttaatgcgtt catgcaaagc ttttaacgcg gtatcgcgtt ccactggcat
     8761 atcacgcaga ccgaaattgg ccatgcagag gttatcgctg ccgattaaaa atacccggtt
     8821 gagatcgtaa gccgcagaaa aattatcgcg ccagtagcgc ataaaccacg ccaatgactc
     8881 cagagaacct cgccaggtgt tactcattgc ggaacaatca gagtcagcaa atagcggttc
     8941 aaacgcaggc acatccgtct gcgtttctcg tccacgcggg gaaagcacac cgttttctgc
     9001 cgataagcga ttttcagcta tgtacttcag ctctttcatc acatcagagg tgcgttgaat
     9061 aaagcgctga gcctgatcgg aactcagatt aaattcctga cgaatttccg attctcgctg
     9121 atgtaacgca ttaacgatgt aaaaaacgga tgaaaaagca atcaacagcc agagcactaa
     9181 cgccaatgct ctgaacatgt agcgcgaggc tttcagggtt gtacgaaaag aagcaaggta
     9241 tttcaaaggg gcgaagctcc gcctcaggtg accgatggag tgtggttaag gtagcggtaa
     9301 aagcgtgtta ccgcaatgtt ctctcttctc tggaatatga tacaccgccg agaaatcatc
     9361 accttaacct ctgataatcg tcatataccg gacaagacta gtggatttca gcatgcatta
     9421 tatgaagtgg atttatccac gccgcttacg taatcaaatg atcctgatgg caatcctgat
     9481 ggtcattgtc ccaacgctta ctattggtta tatcgtagaa acggaaggac gttcagcagt
     9541 cttatctgaa aaagagaaaa aactttctgc cgtggtcaac ctgcttaatc aggcgctagg
     9601 caatcgctac gatctctaca tcgacttacc gcgtgaggag cgtatccgcg cattaaatgc
     9661 agaacttgcc cccattaccg aaaatatcac tcacgccttc cctggcatcg gcgctggtta
     9721 ttacaacaaa acgctggatg cgataatcac ctacgcgcct tcagcgctat atcagaataa
     9781 tgtgggcgtt accattgccg cagatcaccc tggtcgcgaa gtcatgcgta caaatacccc
     9841 tttggtttat tcaggcagac aggtgcgcgg cgatattttg aattcaatga ttcccattga
     9901 gcgtaatggt gaaatcctcg gctatatctg ggccaatgaa ttaaccgaag atattcgccg
     9961 ccaggcctgg aaaatggatg tgaggattat cattgtgctc accgccggtt tgctgataag
    10021 cctgctgttg attgtccttt tctcccgtcg cctgagcgcc aatatagata tcatcaccga
    10081 tggcctctcg actctggctc aaaatattcc cactcgatta ccacaattgc ccggtgaaat
    10141 ggggcaaatc agtcagagtg ttaacaacct cgcccaggca ctgcgtgaaa cgcgaacact
    10201 taacgatctg attattgaaa acgctgccga tggcgtcatt gccattgacc gccagggtga
    10261 tgtaaccacc atgaacccag cagcagaagt catcactggc tatcaacgtc atgaactggt
    10321 agggcagcct tactccatgt tgttcgacaa tactcagttc tacagtcccg tactggatac
    10381 gctggaacat ggcaccgaac atgtagcact ggagatcagt tttccaggcc gtgaccgcac
    10441 cattgaactc agtgtcacga ccagtcgtat tcataacacg cacggtgaaa tgataggtgc
    10501 tttggtgatt ttctctgatt taactgcccg caaagaaacc cagcgccgca tggcgcaagc
    10561 agaacgcctc gccacactgg gtgagctgat ggctggcgtg gcgcatgaag tacgtaatcc
    10621 gctaacagct attcgtggtt atgtacagat cttgcgccaa caaacccgtg acccaataca
    10681 tcaggaatat ctgtccgtag tactcaaaga aatcgattca attaacaaag ttattcagca
    10741 attgctcgaa ttttcacgcc cacgccacag tcaatggcaa caagtcagcc tcaatgcatt
    10801 ggttgaagaa actctggtac tggtacaaac cgccggcgta caagcgcggg tcgacttcat
    10861 aagcgaactg gataatgaat taagcccgat taacgccgat cgtgaactgc tcaaacaggt
    10921 actactgaat atcctgatca atgccgtcca ggctatcagt gcacgaggga aaattcgcat
    10981 tcgaacctgg caatacagcg actcacaaca ggccatttcg atagaggaca acggctgtgg
    11041 cattgatctc tcgctgcaaa aaaagatctt cgatcccttt ttcaccacca aagcctcagg
    11101 aaccgggctt ggtctggcgt taagtcaacg catcattaat gcccatcagg gtgatattcg
    11161 cgtcgccagt ttgccgggct acggcgcaac cttcacgctt attttaccga tcaacccgca
    11221 gggaaatcag actgtatgac tgctattaat cgcatcctta ttgtggatga tgaagataat
    11281 gttcgccgta tgctgagcac cgcttttgca ctacaaggtt tcgaaacaca ttgtgcgaac
    11341 aacggacgca cagcattaca cctgtttgct gatattcacc ctgacgtggt gttgatggat
    11401 atccgcatgc cagagatgga cggcatcaag gcactaaagg agatgcgcag taatgagacc
    11461 cggacacccg ttattctgat gacggcctat gctgaagtgg aaaccgccgt cgaagcgcta
    11521 cgctgcgggg ccttcgacta tgttattaaa ccgtttgatc tcgatgagtt gaatttaatc
    11581 gttcagcgcg ctttacaact ccagtcaatg aaaaaagaga tccgtcatct gcaccaggca
    11641 ctgagcacca gctggcaatg ggggcacatt ctcaccaaca gcccggcgat gatggatatc
    11701 tgcaaagaca ccgccaaaat tgccctttct caggccagcg tcttgattag cggtgaaagc
    11761 ggcaccggga aagagttgat tgccagagcg attcactaca attcgcggcg ggcaaagggg
    11821 ccgttcatta aagtcaactg cgcggcgctg ccggaatcgt tgctcgaaag tgaactgttt
    11881 ggtcatgaaa aaggtgcatt tactggtgca caaaccttgc gtcagggatt atttgaacgt
    11941 gccaacgaag gtactctgct cctcgacgaa attggcgaaa tgccgctcgt actgcaagcc
    12001 aaattactac gcattctgca ggaacgggaa tttgaacgga ttggcggtca tcagaccata
    12061 aaagttgata tccgcatcat tgctgccacc aaccgtgact tgcaggcaat ggtaaaagaa
    12121 ggcaccttcc gtgaagatct cttttatcgc cttaacgtta ttcatttaat actgccgcct
    12181 ctgcgcgatc gccgggaaga tatttccctg ttagctaatc actttttgca aaaattcagt
    12241 agtgagaatc agcgcgatat tatcgacatc gatccgatgg caatgtcgct gcttaccgcc
    12301 tggtcatggc cgggtaatat tcgagagctt tccaacgtca ttgaacgcgc cgtcgtgatg
    12361 aactcaggcc cgatcatttt ctctgaggat cttccgccgc agattcgtca gccagtctgt
    12421 aatgctggtg aggcaaaaac agcccctgtc ggtgagcgta atttaaaaga ggaaattaaa
    12481 cgcgtcgaaa aacgcatcat tatggaagtg ctggaacaac aagaaggaaa ccgaacccgc
    12541 actgcgttaa tgctgggcat cagtcgccgt gcattgatgt ataaactcca ggaatacggt
    12601 atcgatccgg cggatgtata acgccaaaac ttgctatgca gaaatttgca cagtgcgcaa
    12661 ttttctgcat agccgctcat tctccttatg aatccccatc taatttattc ctttctattc
    12721 aattagttaa ataacaacat tcaataatct cattctggca ctccccttgc tattccctga
    12781 ctgtacccac aacggtgtat gcaagaggga taaaaaatga aaacaaaatt gatgacatta
    12841 caagacgcca ccggcttctt tcgtgacggc atgaccatca tggtgggcgg atttatgggg
    12901 attggcactc catcccgcct ggttgaagca ttactggaat ctggtgttcg cgacctgaca
    12961 ttgatagcca atgataccgc gtttgttgat accggcatcg gtccgctcat cgtcaatggt
    13021 cgagtccgca aagtgattgc ttcacatatc ggcaccaacc cggaaacagg tcggcgcatg
    13081 atatctggtg agatggacgt cgttctggtg ccgcaaggta cgctaatcga gcaaattcgc
    13141 tgtggcggag ctggacttgg tggttttctc accccaactg gtgtcggcac cgtcgtagag
    13201 gaaggcaaac agacactgac actcgacggt aaaacctggc tgctcgaacg cccgctgcgc
    13261 gccgacctgg cgctaattcg cgctcatcgt tgcgacacac ttggcaacct tacctatcaa
    13321 cttagcgccc gcaactttaa ccccctgata gcccttgcgg ctgatatcac gctggtagag
    13381 ccagatgaac tggtcgaaac cggcgagctg caacctgacc atattgtcac ccctggtgcc
    13441 gttatcgacc acatcatcgt ttcacaggag agcaaataat ggatgcgaaa caacgtattg
    13501 cgcgccgtgt ggcgcaagag cttcgtgatg gtgacatcgt taacttaggg atcggtttac
    13561 ccacaatggt cgccaattat ttaccggagg gtattcatat cactctgcaa tcggaaaacg
    13621 gcttcctcgg tttaggcccg gtcacgacag cgcatccaga tctggtgaac gctggcggac
    13681 aaccgtgcgg cattttaccc ggcgcagcca tgtttgatag cgccatgtca tttgcgctaa
    13741 tccgcggcgg tcatattgat gcctgcgtgc tcggcggttt gcaagtagac gaagaagcaa
    13801 acctcgcgaa ctgggtagtg cctgggaaaa tggtgcccgg tatgggtggc gcgatggatc
    13861 tggtgaccgg gtcacgcaaa gtgatcatcg ccatggaaca ttgcgccaaa gatggttcag
    13921 caaaaatttt gcgccgctgc accatgccac tcactgcgca acatgcggtg catatgctgg
    13981 ttactgaact ggctgtcttt cgttttattg acggcaaaat gtggctcacc gaaattgccg
    14041 acgggtgtga tttagccacc gtgcgtgcca aaacagaagc tcggtttgaa gttgccgccg
    14101 atctgaatac gcaacggggt gatttatgat tggtcgcata tcgcgtttta tgacgcgttt
    14161 tgtcagccgg tggcttcccg atccactgat ctttgccatg ttgctgacat tgctaacatt
    14221 cgtgatcgcg ctttggttaa caccacaaac gccgatcagc atggtgaaaa tgtggggtga
    14281 tggtttctgg aacttgctgg cgtttggtat gcagatggcg cttatcatcg ttaccggtca
    14341 tgcccttgcc agctccgctc cggtgaaaag tttgctgcgt actgccgcct ccgccgcaaa
    14401 gacgcccgta cagggcgtca tgctggttac tttcttcggt tcagtcgctt gtgtcatcaa
    14461 ctggggattt ggtttggttg tcggtgcaat gttcgcccgt gaagtcgccc ggcgagtacc
    14521 cggttctgat tatccgttgc tcattgcctg cgcctacatt ggttttctca cctggggtgg
    14581 cggtttctct ggctcaatgc ctctgttggc tgcaacaccg ggcaacccgg ttgagcatat
    14641 cgccgggctg atcccggtgg gcgatactct gttcagtggt tttaatattt tcatcactgt
    14701 ggcgttgatt gtggtgatgc catttatcac ccgcatgatg atgccaaaac cgtctgacgt
    14761 ggtgagtatc gatccgaaac tactcatgga agaggctgat tttcaaaagc agctaccgaa
    14821 agatgcccca ccatccgagc gactggaaga aagccgcatt ctgacgttga tcatcggcgc
    14881 actcggtatc gcttaccttg cgatgtactt cagcgaacat ggcttcaaca tcaccatcaa
    14941 taccgtcaac ctgatgttta tgattgcggg tctgctgcta cataaaacgc caatggctta
    15001 tatgcgtgct atcagcgcgg cagcacgcag tactgccggt attctggtgc aattcccctt
    15061 ctacgctggg atccaactga tgatggagca ttccggtctg ggcggactca ttaccgaatt
    15121 cttcatcaat gttgcgaaca aagacacctt cccgatcatg acctttttta gttctgcact
    15181 gattaacttc gccgttccgt ctggcggcgg gcactgggtt attcaggggc cgttcgtgat
    15241 gcccgcagcc caggcgctgg gcgctgatct cggtaaatcg gtaatggcga tcgcctacgg
    15301 cgagcaatgg atgaacatgg cacaaccgtt ctgggcgctg ccagcactgg caatcgccgg
    15361 actcggtgtc cgcgacatca tgggctattg catcactgcc ttgctcttct ccggcgtcat
    15421 tttcgtcatt ggtttaacgc tgttctgacg gcaaccctac agacagaagg aatataaaat
    15481 gaaaaattgt gtcatcgtca gtgcggtacg tactgctatc ggtagtttta acggttcact
    15541 cgcttccacc agcgccatcg acctgggggc gacagtaatt aaagccgcca ttgaacgtgc
    15601 aaaaatcgat tcactacacg ttgatgaagt gattatgggt aacgtgttgc aagccggact
    15661 ggggcaaaat ccggcacgtc aggcgctgct aaaaagcgga ctggctgaaa cggtgtgcgg
    15721 attcacggtc aataaagtgt gtggttcggg tcttaaaagt gtggcgcttg ccgcccaggc
    15781 aattcaggca ggtcaggcgc agagcattgt ggcggggggt atggaaaata tgagtttagc
    15841 gccctactta ctcgatgcaa aagcacgctc tggttatcgt cttggagacg gacaggttta
    15901 tgacgtaatc ctgcgcgatg gtctgatgtg cgccacccat ggttatcata tggggattac
    15961 cgccgaaaac gtggctaaag agtacggaat tacccgtgaa atgcaggatg aactggcgct
    16021 acattcacag cgtaaagcgg cagccgcaat tgagtccggt gcttttacag ccgaaatcgt
    16081 cccggtaaat gttgtcactc ggaagaaaac cttcgtcttc agtcaagacg aattcccgaa
    16141 agcggattct acgactgaag cgttaggcgc attgcgcccg gccttcgata aagcaggaac
    16201 agtcaccgcc gggaacgcgt ctggtattaa cgacggtgct gccgctctgg tgattatgga
    16261 agaatctgcg gcgctggcag caggccttac ccccctggct cgcattaaaa gttatgccag
    16321 cggtggcgtg ccccccgcat tgatgggtat ggggccagta cctgccacga aaaaagcgtt
    16381 acaactggcg gggctgcaac tggcggatat tgatctcatt gaggctaatg aagcatttgc
    16441 tgcacagttc cttgccgttg ggaaaaccct gggctttgat tctgagaaag tgaatgtcaa
    16501 cggcggggcc atcgcgctcg ggcatcctat cggtgccagt ggtgctcgta ttctggtcac
    16561 actattacat gccatgcagg cacgcgataa aacgctgggg ctggcaacac tatgcattgg
    16621 tggcggccag ggaattgcga tggtgattga acggttgaat taatcaataa aaacacccga
    16681 tagcgaaagt tatcgggtgt tttcttgaac atcgacggcg aaggtaaccc cattaatcac
    16741 cagtcaaaac ttttcaccag cgtcagctcg ccagcattac gcatcggtac aataaatgtt
    16801 tcctgtttct cattgaccga tccttcatcg gtgatcagcg tcagttgggc ggtcgtcaat
    16861 tccgtttcgc tgcgtccgcc atagtagttg atatacacct gataacgtcc gtgaatcggc
    16921 gctggcatgg cgaaaatctc gggtccgtac cccgtcgtga catccatatc cagtgcacca
    16981 ctgtttttca gcaccgtgtt accgtaccag gcgtgttcgc catcgggcgt gacaacatgg
    17041 agatcgagat cggtattgtc cgtatcccac gagagaacca gccgtaaacg tgcacgaatc
    17101 gttcctgtac ccggcgttga gtaaaactgc attttttgcc ggctttgccc atccgggctg
    17161 atgacctgca cgctgttgct gccttccgtg aaaatataag ggcgtgcaaa tgaaccatcg
    17221 gattcaattc tctgcggcat actggcaccg ttgaccacca gccggccctg ctgaacttta
    17281 cccgccgcag cataattctt tattttgccg cggatctgcg ctgagatatt ttgatcatct
    17341 gccatgttga cggacgacgc cgggtaattg atcgattgtg aaaaactggc atcttcaccc
    17401 tctgccggat gccagccaga gagcggtgca tcaatctcga cttcctgcac accttcactg
    17461 tgagcgacag gcgaaagcgc caccagtaac aacggaagaa aaatctttcg catagcatta
    17521 gtccattaat aattgtcggg taagcgtttc gatatagttc tcatcaagcc cggtgggata
    17581 accatcaaag gccaggtgca gatactcatg ggttaaatcc agccgatcct gaaacgtaaa
    17641 gaagttacga ataaacagcc gtttctgctg ccgatcggta taggggaaac ctgagactaa
    17701 acggcaaacc gcaaacacgt ctggttcgtt gtaccccgtc tcaccttgta atatacgccg
    17761 ccactgcggc atttttttcg ccagccaggc tttcgctttg ggtaataact ggcaggttga
    17821 gcgtggcgct ccccagcggc taaggctatt gtcgggataa gcaaacgcga gtatctgatc
    17881 gtaacgctct ccctgccccg cctgggccgt ggcttgccgc caggaaagcg tcccttcggt
    17941 agcgcgactg ccgtgataat gaaccggatc gcccgcatag ataagatcct gcgtccaggc
    18001 ggtcatcgtt cgcgcccccg tggtagctgg cgaagcagaa acacgctgcg tggcgctgct
    18061 gtcagggatg gtcaggcaat cgccttcccg attggcgttt tgctgtaaaa aagtgcgtat
    18121 cgccacggtc atggctttcg ctgcctcagg tggcgtactt ttcgcctcac gatccagaac
    18181 tctggcaacg tactcttcac gatcgagatg cgactgcaat ttcagtttgc ccttttcgct
    18241 cagtaaggtc gtctcaccgt ggctgacaaa cataatgtgg ttaccattgg caaacgtcac
    18301 acgatagcgc ccattaagta cgccgggttt tactgccgtc gaactctttt ccgccgtgat
    18361 tttcttcagc ggatagcggg caaacagctc caccaggaca cattgcccac tggcaacttg
    18421 cgtcggcact ggcaaaaccc gattaagcac cgtcgcataa cgggtcagta ccgttttgct
    18481 ggtgccactg ccagtcaccc acagcggtgt accatcggtt aaccagccgg caaaaccacc
    18541 ctgacggatt tcctgtttat catcggcaaa ccagctccag gttttcaccc gtacccgact
    18601 gccgagcatg gatgcgacac cgattttcgc ctcgtccagc accacatcca gtaagacttc
    18661 ctgagctttg ttttgcgcag gtaacgtcgc taatgaatca agcagcgagt taaccgttac
    18721 cgaggtttct ggcttcagcg tagtaagaga cgctaaccac gcaggtgcct gacgctgttg
    18781 ccaatattgc ccccacacat ctgcgccaat atgcaaccgc tgcggcgcaa aatagagccc
    18841 acaggaacgt accagcgcgg tatcgcgcgt tatactctcc ccagcctgac aacaataaac
    18901 ctcttccggg gagtttccgc gacactggta gggctgctcc ggttgatggg tatcctccag
    18961 ccaggcgtaa acatacagtt tccacaaact ccctaacggc gtggtgagcg tgtcaggtaa
    19021 tgccgagact ttggtcacgc ctgatgacga cagctgatac agttgatcgt gctgcgcacc
    19081 gcgcagcacc agttgtaacg gcgtctcctc cgcaagcgtt ggcagagtaa ccaacgccaa
    19141 tagccagacg attctgcgcc agttcactta tttcacctgc atcccggtcc attcgctccc
    19201 tgccgcaaca ctttgctgtg caggtgcata ggaacgcaca taacgagcag gaggcagaac
    19261 gaattgccct ttttgcgaga agcgcagcaa atggcggaaa gtgaccgttc ccgtcagttc
    19321 tttcaccggc accatatagg ccagtttgcc catttcattt cgcgcttttt ccagcaactg
    19381 cccctgctgt tttccagcgt tcggtttatt gaccgaaatg ccccacgtag tcctctcaac
    19441 gtcagcccct ggcgggagcg gtacttccac ctgaccgtag cgcagaacgg catcctgctc
    19501 gctggtaagc gtaatttcat cgagatacag cgcatcgctg tcaatctcat tgctggtcac
    19561 cggctgcaga gtaaagctca tctcttcttc accggggata agccgataca actggcgttc
    19621 aacggtcacc ggaatgttac tttgttgagc cgtttttgcc ggttcacgcc agcggacctg
    19681 cacattttgc ggcgataatt cgtcaccaaa agagagaatg tccggcacac cctgaccaac
    19741 ccaacgccag tcttcgcccc ctccagttaa cttatgttta gcccatgcgc ccgcaggcgc
    19801 aggcaaaaca actggaggca ttgtcgccat atatttcgcc agccagttca tggccagcgc
    19861 gcgctcaata gtggattgct cagcggtcaa accacttaaa atagcggccg cctgggtagc
    19921 gtcaccaccg ctattgagca gcacaaccgt gtgcgccagc ggctgattgc tgtttgccgc
    19981 tttatcccac gcatattgct gaacctgtgg catcaccgtg gacttcaact gcgctttttt
    20041 cagcaacgtc atggttaaga cgtttgccac cgcatctgcc agtggagatt ccggtgtatc
    20101 gaggatcagg ctgtcattga tatcgctggt gtcttcctca gagaaatctt cagttttagt
    20161 tccgcgccgg gcgatagctt catccaaccc tttcaacaac gttttgcacg gtagattcat
    20221 ctcctgtgcc cacgccagca ccagcgcgcg atgcaatatc ggcatgttat ctgcctgctc
    20281 ggcataactg tcgagcatat gttgccagta atccggttgc tgcgttacgc cgagcgcctg
    20341 gctggcctgc cagtcggcgt accatgccca tgccgtcagg aaggcgtcgc cattgccatc
    20401 ttcaccccac caggtaaagc gcgctccggg ccccgccagt tgcatcagcc gcagacggtt
    20461 atcctgaatc atctgacgaa tgtcgttagc ggcggcactt tgatgatcgg caagtgaacg
    20521 ccaggcgaga ctgagcggga tcagacggct accggtgttg atcacgcccc cccacggttc
    20581 atcgactaac gcatcaagat tgttacggaa aatctcctgc ggcgtttcgc tactttgtag
    20641 ctggatattg ctcgcctgct cgggcaacat cagcgcgtta tcgccaccgc cgagcatgac
    20701 gtttttctgc tgttcaacgg gccagctgtt atccacaaaa gacagttttg tgctaatgct
    20761 gtcctgtact tgcccatttt gttgcagttc tgcacttaac aagccagatt gctgaatatt
    20821 ctgcgtcagc gaaatataat tcgccccttt gtgcagcgtc agcgtctggc gcatctcagc
    20881 acctgcaaat ttagtcacca gcgccaccgg ttcgttatcc tgctgactga agataaacag
    20941 tcctgctgcc ggtttgtcgc ccatgcgata caccgttggc atactccact tcatgtagag
    21001 atttttttcc gaacgcagat aagcacgccc ctgcccgacc agcccgtcgc cgttcatccc
    21061 acgcgcggtg atgcgccagc gggttaacga atcaggcatc aggaacgtga aatacgcttt
    21121 gccttgttta tcggttgtga gtgacggcat ccatgccgcg gtatccacct cttcacgccg
    21181 tggacgttca agcattttta ctcgccgctc gctgcggtta gtcgcgccag gcgcaaccgg
    21241 ctcgctggag agcgcctggt cgtagctgat aaacgacaga ctggagctgg tacgcacatt
    21301 gttacgcccc agcggataga aaaatttgcc gatattcggc gcgatttccg gttgcagcgc
    21361 gtagatcatt tcatcgacca caccaaccgt tagctgcgca gaaacaggtt tacctttcag
    21421 cgacgaggtt aattcgacat tgaccagttc accaggctgg taatgggttt tttccgtttt
    21481 cacccggata tccagctggg gaacggcaac tttgatcccg gcgttctgaa aactgtactg
    21541 accgttacgg gtatacagca ccgaaaaagt gatgttaggc gcaaaggaat tgctcactgg
    21601 tatgcgggct tcatactggg tatcgtttaa acgttgtaac gtcagccagt ttgccggatg
    21661 cgatagcagc gactgctgtt caacgcgatc gcgttccagc gtcaataatg cttcatcaat
    21721 tggctccggg aaggtaatca gcatcttcgc ggtttcgcct ggctggtaca gcgttttatc
    21781 cgccacgata tctaccgtac ccgtatgcgc cgtgctgccc ttaccgctga ctgcatggct
    21841 taacccggcg agaattaagc cgtcttcatc gcgtaatgtc agattgtagt tgccaggttt
    21901 atcgaaattg acggtaaagg atttgccgcc tgacggtaga tctccgctat ggctcgtgcg
    21961 gtcttcgaga cgcaaccatt catacgtaac aggaacctgt tttgaagatt ccagcgcggc
    22021 ataacggaac acaaccgact cgccgctatt actgtattgt gcggcggtac ttaaagagta
    22081 atgcgccagg ccgcgttcaa tgaggatctc tttggtggtg gtgacgcgat acgccgcgcc
    22141 gtcactggcg gagacggtta acaaatagcg gctcggttta tcggcggcgg ggagatttaa
    22201 agccacatga ccgctatcat cggacaccgt ttcgctgcct tccagcgaca cgggaaaacg
    22261 tccggcataa cgcaaatcgt taccgaccat tgataattgc tgagcgcgca aacttaactg
    22321 cacgcgggca tcttttaccg gctctccatc cgggtaaagc agttgcagtt tgccgctcac
    22381 cgcttcgcct gttttgaact cttttttgtc gagagctaaa ccaatctcga aatgtggctt
    22441 gatgtagttt gccacgcgaa aactgctgct atagacctgt ttgcggtaag ccaggcgtaa
    22501 ctcataacct ccggcgacgg cattttctgg caggcggaaa ctcccctgcc cgccattacg
    22561 cgcatcaagc gtgacattga cggtttgcaa cagactgccg ttggcgtcca gcactgaaag
    22621 cttcgccggg gcgctgacga tgggggatga atgcaacggg tcgtggaact cgcggcccat
    22681 cactttgaca tcaacacgat cgcctgcacg atacagaggg cggtcggtaa agatatacaa
    22741 gcgggtgttg tagatttcgc tttcgtagaa aaaattctcg gagacaaaaa cgccgccttc
    22801 agcatcttta cccagaatgt atgaacgttc cggcgatata tgttgtaact gcaaggtacc
    22861 gctgtcatcg gtcacaccgc gggtcatcac gccaagaccg tcagtccaca agatctctga
    22921 tcccggcttc gcttccccct gttttttacc cgcggtccac accagaagct cgttacctga
    22981 cactttgcta agcgccacgg tatcggaaac aaacaccacc gtcgtcgccc gatacccacc
    23041 taccatcgcc tcgacgaggt acagccccgg ctcttgcttg cctagcggaa tatagatgtt
    23101 gcccggctgc ggcgagatga aattgctgga tgcgccttcc agttttaccc cttgctgcgg
    23161 ctcgaccggt ttagcctgcc ataatgggta acggaactgt tccaccaggg gatatttttt
    23221 cagcggtgaa aactggttgt tctgtacata acgggaaggt ttaataatgg cattaccgag
    23281 ctgtaattcg ggtaacgcct gagtcacatt ctgccgagac tgagaagaaa aagtacgctg
    23341 catcacgcgg cgagatttgc cgtaccagtt atcccacagc caggtcagcg tattgttcag
    23401 cccgtcgccc aaatattgcg gttgcaccac aatgcgatgc aggtttttct gctggcgcaa
    23461 aaatgccatc gggtcaggaa tacgatacag gcgaacgtcc acgccgccgt actcttccat
    23521 ctgatagcgt cgataatcac gccccggcgc ttccagtcgc actttcgcct cttcactgct
    23581 gctaaaactg ctgtcagcga gcaaaaagaa tgttcccccg gcgggcggcg catagttgct
    23641 ggaaggaagc gaatcatcag cattggcaag ccctgttccc acaagagaca ggaacaagca
    23701 cgctgctatc gcgccagaaa atttaaacga taaatgccaa tgaaattggg attggaatcg
    23761 ttgggtatcc atcgggtgtc cttccatgtc ataagttgtt gcagactgac tgcgcgcatt
    23821 ccgttgtcag ttttcgtggc gcttccggtg tggtagatga cgtaacgccc catccagacc
    23881 attaagtgct gggcatcgcc ctgatcgaaa aaaatcatat cgccaggcag cgcctggttt
    23941 atgtcctggc caataaattg gctgttgtac tgaatcaaat taattgcggt cacgtagggg
    24001 ccggttttcc cgttcccctg attccagttt tgcgccagtt gccgctgttc aggtgttagc
    24061 gtcatctctg gcggcaaata ctggttagat aacccgttac tttttagcca tttactgtcg
    24121 tgaactttca gcgtctcgtt cgctgcaaat cgcaccaggc ccgcacaatc ctgctgatac
    24181 cagcgtggac ttggaccctg gcggagctgt tcctgtgcaa tgcgtacaaa ccaggcgcga
    24241 aacagcccgg attgttcgac attcagcatt tcgctatggg taacaacaca acacagccag
    24301 caaatcagcg ccagcagccc gtgtctcata gcggttgcca ggttatgggt agccactgcc
    24361 aggcggcacc gggttccatc tgggccagct tcatgacata acgcggttgt tgagataaag
    24421 cgtccagctt cggcattaat aaagtttgtg cggcgttata aaaaaccggt tcgagattct
    24481 tcggcaaact ggtcagcgtt tcgttacgca gcagtttcgc cacgccttgt ggattgatat
    24541 agagcggaac gatgccgtca gtgggtatca catccaccat tgccgggcgg gttttattca
    24601 gtgtttgcag cgcgttatta accagcgtgt catcgaggga gaaaagcatc gtcttgtttt
    24661 gcatcgccag cgacacgcgg aaaaaataat ccgacattaa ttgatcgggt tgcaccgcct
    24721 gcgctttcgg atactggccg tatcgggaac tcacttcgcg acgccagatt tgcgcttcgc
    24781 cctgctgagt ctggtttacc ggcaaaacac cttctggcgc tttgctttcg tgcgcaccaa
    24841 tattttgcgt aaacagtttc ccaggcagtt gtgcctgttc ggctgagcca tcaaattgac
    24901 caacaaatag cggggtttgt aattttgagt cttcatacca gcacagcccg gctgcaccat
    24961 ctaacgcccc gttcagcttg tcgttttcct ggctgatgtg cgaaagcatc tcttcggcaa
    25021 taccatgcga atacggcacc gccacacaga agctggcccc ggcaggcata ctgttccata
    25081 ccggcgtaaa atcgaaactg gcatctacgc tggcggattc atcatttaac gccacaaagc
    25141 tatgccagcc gtcgttgctc atttcgaagc gtacgccagc aaaagaaggc attaatcgct
    25201 ggtagccaaa ccccagcaac ctggcgctga caacaatacg ctggcgtact ggcgtttttt
    25261 cagcagcacg ctcttccagg ccaaagctgg cttgccagcg ttttttaccg ctcaacaaat
    25321 cacttgcgat cgccgtggct tcggtatcct gctggtcatc tttaaacagc atatccgtac
    25381 tggaaaacac cagcatcttg tcctgataag tcgcgaacat cagggcgtta ttgccgttat
    25441 agcgcaactg ataaaccggg atggtttcac tatttagctt aatactactg atttccgttt
    25501 tgcttaactg gctgtcgctg gtagcggcaa acaacaatgg ctccaacagt ttgcttaaac
    25561 cactgcgctg gatcagcacc atgtaatgtg aaagatggcc ttgtttatcg tgccatagcg
    25621 ccgcctgcgc gggctgatct aagagtgacg aaaagagctt atctttcagc gtgagatcgt
    25681 gttcatagac aatgcggcga atgctgcctt caatgcccag acgatcggca tgattctgat
    25741 aatagaaaac gaaatcttca ctcagaacat cgtggagaaa cggaatggta aggaggtctt
    25801 tgggaagctg gctcagagag tcgctgtcga gaaagaggtc cggctcattg agatcgattt
    25861 gcagattgtt gtgcaccacc agcggcgaca acgttttttc tggcccactg ccagcatatt
    25921 gcaacgccca gacgccagcg gaaagcagta ctattgcgcc caaacctaca agaccataga
    25981 accgccagcc tttcgccttt ttttcaccac tcattgccac attccttgtg taaagccagc
    26041 cattttttac gagcacagca aaactttacc gcgccctaat acgacaaaag cccagacttt
    26101 gcagcctgga cttttcaatt caaacaaggg agatagctcc cttttggcat gaagaagtaa
    26161 aattattctt cttctggctc gtcgtcaacg tccacttccg gagcgatttc atcgtcccct
    26221 tccgcggcac tgccatcgat ggtatccaga tcttcctcgt caaccggttc agcaacacgt
    26281 tgcagaccca ctacgttttc atcttccgca gtacggatga ggatcacgcc ctgggtgtta
    26341 cggcctacga tgctgatttc cgaaacgcga gtacgtacca gcgtaccggc atcggtgatc
    26401 atcatgatct ggtcgcagtc atctacctgc accgcgccaa ccactaaacc gttacgttcg
    26461 gtaaccttga tggagataac ccctttcgtc gcacgcgact tagtcgggta ttccgccact
    26521 gcggtacgtt taccataacc gttctgcgtt gcggtgagga ttgcgccatc gccacgaggc
    26581 acgatcagag agacgacttt atcgccttcg cctaaacgaa taccgcgaac accggtggtg
    26641 ttgcagccca tcgcacggac agaagactct ttaaagcgca ccactttacc ttcggcggag
    26701 aacagcatta cttcgtcttc gccgctggtc aggtcaacgc cgatcagctc atcgccttca
    26761 accagtttga tcgccacttt accggcggta cgcagacggt tgaactcggt gaggacggtt
    26821 ttcttcacag taccgttagc ggtcgccatg aagactttca cgccttcttc aaactcggtc
    26881 accggcagga tcgcagtgat acgttcgtcc tgctccagcg gcagcaggtt gacgatcgga
    26941 cgaccacgcg cgccacgagt ggcttctggt aactggtaga ccttcatcga atagacgcgg
    27001 ccacggctgg agaagcacag aatatggtcg tgggtgttcg ccaccagcag tcggtcgata
    27061 aagtcttctt ctttaatacg tgcggcagat ttacctttcc cgccacgacg ctgcgcttca
    27121 tattcagaaa gcggctgata cttcacgtaa ccctggtgag aaagcgtgac caccacatct
    27181 tcctggatga tcagatcttc caggttgatg tcggcgctgt tagcggtgat ttcagtacga
    27241 cgtttgtcac cgaactgttc acgaaccagt tccagctctt cgcggatcac ttccatcaga
    27301 cgatcggcac tgccaagaat acgcagcagt tctgcgatct gatccagcag ctctttgtat
    27361 tcgtcgagca gtttttcatg ctcaaggccg gtcagtttct gcaaacgcag atccagaatc
    27421 gcctgagctt gctgttcggt caggtagtac agaccatcac gcacgccgaa ctctggctcc
    27481 agccattccg gacgcgcagc atcatcgccc gcacgttcga gcatggcggc aacgttgccc
    27541 agctgccacg gattagccac cagcgcagtt ttcgcttctg caggcgtcgg cgcatgacgg
    27601 atcagttcga tgatcgggtc gatgttcgcc agcgctacgg ctaatgcttc aaggatatga
    27661 gcacgatcgc gagctttacg cagttcgaaa atagtacgac gagtcaccac ttcgcggcgg
    27721 tgacgaacaa acgccgcgat gatgtctttc aggttcatga tcttcggctg accatggtgc
    27781 aatgccacca tgttgatacc gaaagaaacc tgcaactggg tctgggagta gaggttgttg
    27841 agcacaactt caccgaccgc atcgcgtttc acttcaatca cgatgcgcat accgtctttg
    27901 tcagactcgt cacgcagcgc gctgatgcct tccacgcgtt tttcttttac cagttccgca
    27961 atcttctcga tcaggcgcgc tttgtttacc tgatacggaa tttcgtggac gataatggtt
    28021 tcacgtccgg ttttggcgtc aacttccact tcagcacggg cgcggatata taccttgccg
    28081 cgaccggtac ggtaagcttc ttcaataccg cgacgaccgt taatgattgc cgccgtcggg
    28141 aagtccggcc cggggatgtg ttccatcagc ccttcaatgc tgatgtcttc atcatcgata
    28201 tacgccagac aaccgttgat gacttccgtc aggttgtgcg gcgggatgtt ggttgccata
    28261 cctacggcga taccggaaga accgttcacc agcaggttag gaattttggt tggcatgacg
    28321 tcgggaattt tttccgtgcc gtcatagtta tctacgaaat cgaccgtctc tttttcgaga
    28381 tcggccatca gttcatgggc aattttcgcc agacggattt ccgtataacg cattgccgcc
    28441 gcagagtcgc cgtcgatgga accgaagtta ccctgaccgt ctaccagcat gtaacgcagc
    28501 gagaatggct gcgccatacg gacgatcgtg tcataaaccg ccgagtcacc atggggatgg
    28561 tatttaccga ttacgtcacc aacgacacgg gcagattttt tataggcttt gttccagtca
    28621 ttgcctagta cgttcatggc gtaaagtacg cgacggtgta ccggcttcag gccatctcgg
    28681 acatctggca gcgcacggcc aacaatgacc gacatcgcat aatccagata ggagctcttc
    28741 agctcttcct caatgttgac cggtgtaatt tctctcgcaa ggtcgctcat ctaaccgcta
    28801 tccctctact gtatcccgga ttcaaaggtc gcaaattata acacagccgc gcagtttgag
    28861 gtaaacctat acgctttatt cacctccaat gcctgatata ctcgtttgtc ttgccaatta
    28921 cggagtagaa gtgccaatga atgccgaaaa atcgccggta aaccataacg tagaccacga
    28981 agagatcgct aaatttgaag ctgtcgcctc ccgctggtgg gatcttgaag gtgagttcaa
    29041 accgctgcac cgcattaacc cgctgcgtct gggctatatt gccgagcgtg ctggcggttt
    29101 atttggcaaa aaggtgctcg atgtcggttg tggcggcggc attctggccg agagtatggc
    29161 gcgcgaaggc gcgacggtga ccggtctgga tatgggcttt gagccattgc aggtggcaaa
    29221 gttgcacgcg ctggaaagcg gcattcaggt agagtacgta caggaaaccg tggaagagca
    29281 cgcggcaaaa catgccgggc agtatgatgt agtgacctgc atggagatgc tggagcacgt
    29341 ccccgatccg cagtcagtgg tcagagcctg tgcgcaactg gtgaaaccag gcggcgatgt
    29401 ctttttctcg acgcttaacc gcaacggcaa gtcatggctg atggcggtgg ttggtgctga
    29461 atatattttg cgcatggtgc ccaaaggcac gcatgatgtg aagaagttta ttaaaccggc
    29521 agaattgctg ggctgggtgg atcagaccag tttaaaagag cggcatatga ccgggctgca
    29581 ttacaacccg atcactaatt cctttaagct cggccccggc gtggatgtga actatatgct
    29641 gcacacgcag aataagtgag gttgttgttt ggattcatcc gacaaccgtg ccaatgcctg
    29701 atgcgacgct tgccgcgtct tatcaggcct acaaatgctc ccgtaggcca gataaggcgt
    29761 ttacgccgca tccggcaaaa gtgccggtca gacagcgatt accatttcac cgtcatcgac
    29821 aaaaaccctg ccgtctgggc aaaatcatca ctccctttct gccacgccac gctgccgcgc
    29881 agggacactc gctgactgat attgcccgtg actcccactt ttatttcacc ccgttgcttc
    29941 accccatcgt cactgatagt gctgccatcc tcttcaattt ccgtcgcatg gggatcgtga
    30001 taataattca gatctaacgt tggcgtgaca ccaacagcag tactccattc gctgtgtaaa
    30061 cccagccgcg tctgaatatc atcaccctgc gattgtgaca cgcgcgcatg gttagcggcg
    30121 gtaaaatcat cctgctgcac gccctgataa atcacctgcg tctgcggttc aatcaccaca
    30181 ccatgccccg gtaaccactg ataaccggct tccagcgagg cgataatccc cgacgagtgg
    30241 taatgatcct cgccatcgtc ttgttcggaa acatcattgt taaaccacgc atattgcagc
    30301 cagctatcca gccagacccc ctgcttctga ttaccgtgct gataccagct tgaggtcagc
    30361 ccaacggcat aaccgtggtt ctggttatcg gcgcgagttc cggtcatatt cgagcggctg
    30421 tcgccctggt tatcgctgta gccaccaaca atcccaagca tccactcgcc atcatcaccc
    30481 cagtgcccgc taaacaggtt gccgctaagc tgcaccgtag aagtgtcttc atgctgagcc
    30541 aactgccctg ccgccgtgta atgataacgc cctccgataa cgcgtaaatt cagcgtctga
    30601 ccatcgccac cagcctgatc gcgtcgctcc atcacaaatg cctgatttgc cgcacgtagg
    30661 ttattaaaat atccaccaac tttggcattc aacaccggct ggtaagcagg cgtaggtcca
    30721 gggtcggggg ttgggtcggg atccggcgtg ggatcaggat ctggagtcgg gtccggatcg
    30781 ggcggcggcg tcggagtgac ttcttgcgat cgcagatacc agtcgttgtt atcttccacc
    30841 agcgtatagt catacgcccc catattgacg tagccgcttc ctgccagacg gaactgcgca
    30901 ttgttttgaa actgcgtggg atcagctgcg aaatcaacca ctttaatacc tgtagatgtc
    30961 ggctcaccaa tacccgtaat ggggttaatt accacggccg tgttgccagc agtattaccg
    31021 ttcaatacca gttggtcact tgccgagtca tcgccgttta attcgctgtc gagcagtagc
    31081 gtaccgccgc cggtataatc accatttacc gtcagcgtgt cgccagcgac gccattttgc
    31141 aggctaatcg caccggacgt attggtcaga ttgccattaa ccgtcacgtc ggaaagataa
    31201 gtcaggctgt cagtttcaag gttggtatta agctcgcctg tcagcgtcac gccatcggtg
    31261 gcattcacca acgtaccgtc gccgctaaac agcgaggcat ccagcacctg gttcgtatcc
    31321 tgctgcgtca atcgcaacac agtaccatcg gttatgtcga tatcgccgcc agaggtggca
    31381 tcgattgagc gaatatcctg attttgcccg gtttcaaaaa caccgtcttc ccccacccac
    31441 agtgaagaaa cataaggaat aatattctcc gcttcacctt ttagcgttcc gtcctccaca
    31501 cgtaccgcag acgtcgcagt gccgctggcg gtcaactcca gcgtaccgcc gccactttta
    31561 gtcaacgtgc ttacgctctc cagttgttgc ccggtactat ccgccatcaa cccgccccat
    31621 tgcgtatcta cgccatcctc aacgtgtatt tcgccatcag cgcgcatttc aatattcctg
    31681 ccctgacctg ttgtagccga gcgagtgtca acatcagtcg tcacatccat ttcactgtgt
    31741 tgttggggat ctgtgaagat cactgaacgg ttgtaacttg tgtcccccaa ttgcgaatta
    31801 tcgctgactt caagcgtacc ggaggcgatt tgcgtattac cgagataacc gttgttattc
    31861 gccagcgtta cgcgctgatc ccccgcatta atgacgacat aaacgccatt ctggctggca
    31921 tcactgccgc caatatgtaa atcatcgcca ctgctgatat caacgacatc attactgcca
    31981 atcactgtcc ccccgctgcc gctgatttgc agaccgtcat tgtatgagct atcgccatcc
    32041 caggtggtga aatcagagag atccaatact ccgccattta gcacgatcga ttgcggatcg
    32101 ctctgcattg cccgtaaatc ggcctgattc cccgctagcg acaatacggc gttatcttcc
    32161 accacaatat cgccggttaa tgccatcgac tgcgcccctg tcaggagata gctgccatct
    32221 tgcgctaccg tcaactgtcc ttcaccctga atcgagccgg aaaaaccgcc ctggtttacg
    32281 gtaacattgc cgcctgcgtc gatattcaaa atgccattag caaaaccggt taatgagtgc
    32341 acaaacgttt gttgtgtatt gccaacattc agttctgcct gatactcaga atgaacagtg
    32401 ctgccgacca tcagaccaaa gcaatcctgc ggatcgctct gacagtgcgt gtcgccgaca
    32461 ttcattaact catcgctgcg ccccaacgtc acctcgccat tctggatact tatctcgccg
    32521 gtaaacgcat tattgtcggc attgaggact aactctcccg ctccctcttt aacgatgact
    32581 cccgttccgg caagagaatc aatcgcgcca tcgttgctgg tattgcctat taccagcgtt
    32641 ttatcggcgg caatgttaaa ttcagccaca ctatgaccga tatacataaa gccccccgcc
    32701 gctgccgtcg ccgaatggtc atatcccgag gcgctgttat tttcatcaat catcacacca
    32761 ttgttttggt cataattatc atcaacagaa atatcaataa gatacgggga tgtggtgctg
    32821 ctggtataaa ttgctccgcc ataaccttct gcatagttat tggtaaagga ggtattatta
    32881 attatcgttt ttcctgaaag atgtgtgtta tccgtactat tatccgtaac atcaatagcg
    32941 ccgccatccc catcactata acttgttgat gtatatgcct ggttattatt aaaagcatca
    33001 tcacttaaat agacgtcatt attgatggta taaattgcac cgcctttgcc gtcattagcg
    33061 atgttattgt gaaatacggc gttagtgaca cgtaaatcgg cggcaccggt atcattggta
    33121 ccggcagaat agattgctcc gccatagcct cctgcaacat tactgtcgaa aataacattc
    33181 gcaagattta tcgttgagtt ttctttagca aagatagccc caccattatt atattcgcca
    33241 ctaaccgagt tatcagcaaa cagggtcatc cctgtttcat tttgtggtga aatcgtaaat
    33301 tcagcacttt gctgcaaaaa tactgcgcca ccgctggcat tattggccat acccgaaaaa
    33361 atcaaccatt gcccatccgc gatgctccag tcctgcatat tgcccgaaag gctttgcctg
    33421 ctggcctgac agttcgcttt gatatcatat ccctggcatg aatcgatgac cgctgcggca
    33481 ccgttaccag agaaaagaga tgcaatcatt gacgggagta aagataaata ctccttgcgt
    33541 agaaagataa tccgcatatt catctaacca tcattttcta taagatggcg tatttaatcg
    33601 cattatgcga tatagggtat ttttcttttg taaattctgc ccctgatgta ataactattt
    33661 tgttattatc tgataattaa tttagatgca tcacaattca aa
//
LOCUS       DAZFLD010000021        33482 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.021, whole genome shotgun sequence.
ACCESSION   DAZFLD010000021 DAZFLD010000000
VERSION     DAZFLD010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33482
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.021"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            405..1622
                     /gene="alaA"
                     /locus_tag="ACHKBL_000912"
     CDS             405..1622
                     /gene="alaA"
                     /locus_tag="ACHKBL_000912"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase AlaA"
                     /protein_id="HFO5817825.1"
                     /translation="MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPA
                     PFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGN
                     GVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
                     IRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDA
                     EHHSIAPLAPDLLTITFNGLSKTYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRL
                     CANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIPGVSCVKPRGALYM
                     FPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSL
                     SKFARFLSGYHQL"
     gene            1706..2305
                     /gene="yfbR"
                     /locus_tag="ACHKBL_000913"
     CDS             1706..2305
                     /gene="yfbR"
                     /locus_tag="ACHKBL_000913"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002953 - 5'-deoxynucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HFO5817826.1"
                     /translation="MKQSHFFAHLSRLKLINRWPLMRNVRTENVSEHSLQVAMVAHAL
                     AAIKNRKFGGNVNAERIALLAMYHDASEVLTGDLPTPVKYFNSQIAQEYKAIEKIAQQ
                     KLVDMVPEELRDIFAPLIDEHAYSDEEKSLVKQADALCAYLKCLEELAAGNNEFLLAK
                     TRLEATLEARRSQEMDYFMEVFVPSFHLSLDEISQDSPL"
     gene            complement(2364..4196)
                     /gene="yfbS"
                     /locus_tag="ACHKBL_000914"
     CDS             complement(2364..4196)
                     /gene="yfbS"
                     /locus_tag="ACHKBL_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HFO5817827.1"
                     /translation="MNGELIWVLSLLAVAIVLFATGRVRMDAVALFVIVAFALSGTLT
                     VPEVFSGFSDPNVVLIAALFIIGDGLVRTGVATVMGTWLVKVAGNSEIKMLVLLMLTV
                     AGLGAFMSSTGVVAIFIPVVLSVAMRMQTSPSRLMMPLSFAGLISGMMTLVATPPNLV
                     VNSELLREGYHGFSFFSVTPIGLVVLVLGILYMLVMRFMLKGDTQTPQREGWTRRTFR
                     DLIREYRLTGRARRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVN
                     GVSEFRARDVLLIDMSAADVDLRQFCSEQLLEPMVLRGEYFSDQALDVGMAEISLIPE
                     SELIGKSVREIGFRTRYGLNVVGLKRNGVALEGSLADEPLLLGDIILVVGNWKLIGML
                     AKQGRDFVALNLPEEVSEASPAHSQAPHAIFCLVLMVALMLTDEIPNPVAAIIACLLM
                     GKFRCIDAESAYKSIHWPSIILIVGMMPFAVALQKTGGVALAVKGLMDIGGGYGPHMM
                     LGCLFVLSAVIGLFISNTATAVLMAPIALAAAKTMGVSPYPFAMVVAMAASAAFMTPV
                     SSPVNTLVLGPGNYSFSDFVKLGVPFTIIVMAVCVVMIPMLFPF"
     gene            complement(4283..4933)
                     /gene="hxpA"
                     /locus_tag="ACHKBL_000915"
     CDS             complement(4283..4933)
                     /gene="hxpA"
                     /locus_tag="ACHKBL_000915"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpA"
                     /protein_id="HFO5817828.1"
                     /translation="MRCKGFLFDLDGTLVDSLPAVERAWSNWARRHGLAPEEVLAFIH
                     GKQAITSLRHFMAGKSEADIAAEFTRLEQIEATETEGITALPGAIALLNHLNKAGIPW
                     AIVTSGSMPVARARHKIAGLPAPEVFVTAERVKRGKPEPDAYLLGAQLLGLAPQECVV
                     VEDAPAGVLSGLAAGCHVIAVNAPADTPRLNEVDLVLHSLEQITVTKQPNGDVIIQ"
     gene            complement(4944..5438)
                     /gene="yfbU"
                     /locus_tag="ACHKBL_000916"
     CDS             complement(4944..5438)
                     /gene="yfbU"
                     /locus_tag="ACHKBL_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HFO5817829.1"
                     /translation="MEMTNAQRLILSNQYKMMTMLDPANAERYRRLQTIIERGYGLQM
                     RELDREFGELKEETCRTIIDIMEMYHALHVSWSNLQDQQSIDERRVTFLGFDAATEAR
                     YLGYVRFMVNVEGRYTHFDAGTHGFNAQTPMWEKYQRMLNVWHACPRQYHLSANEINQ
                     IINA"
     gene            complement(5522..5977)
                     /gene="yfbV"
                     /locus_tag="ACHKBL_000917"
     CDS             complement(5522..5977)
                     /gene="yfbV"
                     /locus_tag="ACHKBL_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminus macrodomain insulation protein YfbV"
                     /protein_id="HFO5817830.1"
                     /translation="MSTPDNRSVNFFSLFRRGQHYSKTWPLEKRLAPVFVENRVIKMT
                     RYAIRFMPPIAVFTLCWQIALGGQLGPAVATALFALSLPMQGLWWLGKRSVTPLPPAI
                     LNWFYEVRGKLQESGQVLAPVEGKPDYQALADTLKRAFKQLDKTFLDDL"
     gene            6315..7517
                     /gene="ackA"
                     /locus_tag="ACHKBL_000918"
     CDS             6315..7517
                     /gene="ackA"
                     /locus_tag="ACHKBL_000918"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HFO5817831.1"
                     /translation="MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARI
                     KWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSS
                     VVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
                     SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVS
                     AIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGL
                     LGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVVFTGGI
                     GENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIA
                     QDASRLTA"
     gene            7592..9736
                     /gene="pta"
                     /locus_tag="ACHKBL_000919"
     CDS             7592..9736
                     /gene="pta"
                     /locus_tag="ACHKBL_000919"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFO5817832.1"
                     /translation="MSRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPR
                     TGGDAPDQTTTIVRANSSTTTAAEPLKMSYVEGLLSSNQKDVLMEEIVANYHANTKDA
                     EVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFG
                     GAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSSKAKVNNVDPAKLQESSPLPVL
                     GAVPWSFDLIATRAIDMARHLNATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSL
                     LVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCERAFATGLPVFMVNT
                     NTWQTSLSLQSFNLEVPVDDHERIEKVQEYVANYINADWIDSLTATSERSRRLSPPAF
                     RYQLTELARKAGKRIVLPEGDEPRTVKAAAICAERGIATCVLLGNPAEINRVAASQGV
                     ELGAGIEIVDPEVVRESYVGRLVELRKNKGMTETVAREQLEDNVVLGTLMLEQDEVDG
                     LVSGAVHTTANTIRPPLQLIKTAPGSSLVSSVFFMLLPEQVYVYGDCAINPDPTAEQL
                     AEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDGP
                     LQYDAAVMADVAKSKAPNSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQG
                     MRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ"
     gene            9926..11446
                     /gene="yfcC"
                     /locus_tag="ACHKBL_000920"
     CDS             9926..11446
                     /gene="yfcC"
                     /locus_tag="ACHKBL_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HFO5817833.1"
                     /translation="MSAITESKPTRRWAMPDTLVIIFFVAILTSLATWVVPVGMFDSQ
                     EVQYQVDGQTKTRKVVDPHSFRILTNEAGEPEYHRVQLFTTGDERPGLMNFPFEGLTS
                     GSKYGTAVGIIMFMLVIGGAFGIVMRTGTIDNGILALIRHTRGNEILFIPALFILFSL
                     GGAIFGMGEEAVAFAIIIAPLMVRLGYDSITTVLVTYIATQIGFASSWMNPFCVVVAQ
                     GIAGVPVLSGSGLRIVVWVIATLIGLIFTMVYASRVKKNPLLSRVHESDRFFREKQAD
                     VEQRPFTFGDWLVLIVLTAVMVWVIWGVIVNAWFIPEIASQFFTMGLVIGIIGVVFRL
                     NGMTVNTMASSFTEGARMMIAPALLVGFAKGILLLVGNGEAGDASVLNTILNSIANAI
                     SGLDNAVAAWFMLLFQAVFNFFVTSGSGQAALTMPLLAPLGDLVGVNRQVTVLAFQFG
                     DGFSHIIYPTSASLMATLGVCRVDFRNWLKVGATLLGLLFIMSSVVVIGAQLMGYH"
     gene            complement(11479..12021)
                     /gene="yfcD"
                     /locus_tag="ACHKBL_000921"
     CDS             complement(11479..12021)
                     /gene="yfcD"
                     /locus_tag="ACHKBL_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase YfcD"
                     /protein_id="HFO5817834.1"
                     /translation="MEQRRLASTEWVDIVNEENEVIAQASREQMRAQCLRHRATYIVV
                     HDGMGKILVQRRTETKDFLPGMLDATAGGVVQADEQLLESARREAEEELGIAGVPFAE
                     HGQFYFEDKNCRVWGALFSCVSHGPFALQEDEVSEVCWLTPEEITARCDEFTPDSLKA
                     LALWMKRNAKNEAVETETAE"
     gene            complement(12079..12630)
                     /gene="yfcE"
                     /locus_tag="ACHKBL_000922"
     CDS             complement(12079..12630)
                     /gene="yfcE"
                     /locus_tag="ACHKBL_000922"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HFO5817835.1"
                     /translation="MKLMFASDIHGSLPATERVLELFAQSGAQWLVILGDVLNHGPRN
                     ALPEGYAPAKVAERLNEVAHKVIAVRGNCDSEVDQMLLHFPITAPWQQVLLEKQRLFL
                     THGHLFGPENLPALNQNDVLVYGHTHLPVAEQRGEIFHFNPGSVSIPKGGNPASYGML
                     DNDVLSVIALNDQSIIAQVAINP"
     gene            complement(12686..13330)
                     /gene="yfcF"
                     /locus_tag="ACHKBL_000923"
     CDS             complement(12686..13330)
                     /gene="yfcF"
                     /locus_tag="ACHKBL_000923"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HFO5817836.1"
                     /translation="MSKPAITLWSDAHFFSPYVLSAWVALQEKGLSFHIKTIDLDSGE
                     HLQPTWQGYGQTRRVPLLQIDDFELSESSAIAEYLEDRFAPPTWERIYPLDLENRARA
                     RQIQAWLRSDLMPIREERSTDVVFAGAKKAPLTAEGKASAEKLFAMAEHLLALGQPNL
                     FGEWCIADTDLALMINRLVLHGDEVPERLVDYATFQWQRASVQRFIALSAKQSG"
     gene            13466..14113
                     /gene="yfcG"
                     /locus_tag="ACHKBL_000924"
     CDS             13466..14113
                     /gene="yfcG"
                     /locus_tag="ACHKBL_000924"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSH-dependent disulfide bond oxidoreductase"
                     /protein_id="HFO5817837.1"
                     /translation="MIDLYFAPTPNGHKITLFLEEAGLDYRLIKVDLGKGGQFRPEFL
                     HISPNNKIPAIVDHSPADGGEPLSLFESGAILLYLAEKTGLFLSHETRERAATLQWLF
                     WQVGGLGPMLGQNHHFNHAASQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYS
                     IADIACWPWVNAWTRQRIDLAMYPAVKNWHERIRSRPATGQALLKAQLGDERSDS"
     gene            14170..14532
                     /gene="folX"
                     /locus_tag="ACHKBL_000925"
     CDS             14170..14532
                     /gene="folX"
                     /locus_tag="ACHKBL_000925"
                     /EC_number="5.1.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate 2'-epimerase"
                     /protein_id="HFO5817838.1"
                     /translation="MAQPAAIIRIKNLRLRTFIGIKEEEINNRQDIVINVTIHYPADK
                     ARTSEDINDALNYRTVTKNIIQHVENNRFSLLEKLTQDVLDIAREHHWVTYAEVEIDK
                     LHALRYADSVSMTLSWQR"
     gene            14553..15446
                     /gene="yfcH"
                     /locus_tag="ACHKBL_000926"
     CDS             14553..15446
                     /gene="yfcH"
                     /locus_tag="ACHKBL_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HFO5817839.1"
                     /translation="MKIVITGGTGLIGRHLIPRLLELGHQITVMTRNPQKASSVLGPR
                     VALWQGLADQSNLNGVDAVINLAGEPIADKRWTHEQKERLCQSRWNITQKLVDLINAS
                     DTPPSVLISGSATGYYGDLGEVVVTEEEPPHNEFTHKLCAHWEEIACRAQSDKTRVCL
                     LRTGVVLAPDGGILGKMLPPFRLGLGGPIGSGRQYLAWIHIDDMVNGILWLLDNELRG
                     PFNMVSPYPVRNEQFAHALGHALHRPAILRVPATAIRLLMGESSVLVLGGQRALPKRL
                     EEAGFAFRWYDLEEALADVVR"
     gene            complement(15494..16396)
                     /gene="rpnB"
                     /locus_tag="ACHKBL_000927"
     CDS             complement(15494..16396)
                     /gene="rpnB"
                     /locus_tag="ACHKBL_000927"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnB"
                     /protein_id="HFO5817840.1"
                     /translation="MTISTTSTPHDAVFKSFLRHPDTARDFIDIHLPAPLRKLCDLTT
                     LKLEPNSFIDEDLRQYYSDLLWSVKTQEGAGYIYVVIEHQSKPEELMAFRMMRYSIAA
                     MQNHLDAGYKELPLVIPMLFYHGCRSPYPYSLCWLDEFAEPAIARKIYSSAFPLVDIT
                     VVPDDEIMQHRKMALLELIQKHIRQRDLLGLVDQIVSLLVTGNTNDRQLKALFNYVLQ
                     TGDAQRFRAFIGEIAERAPQEKEKLMTIADRLREEGRNDGLILGKREEALRIAQEMLE
                     RGLDRELVMMVTRLSPDDLIAQSH"
     gene            complement(16593..17366)
                     /gene="hisP"
                     /locus_tag="ACHKBL_000928"
     CDS             complement(16593..17366)
                     /gene="hisP"
                     /locus_tag="ACHKBL_000928"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter ATP-binding protein
                     HisP"
                     /protein_id="HFO5817841.1"
                     /translation="MSENKLNVIDLHKRYGEHEVLKGVSLQANAGDVISIIGSSGSGK
                     STFLRCINFLEKPSEGSIVVNGQTINLVRDKDGQLKVADKNQLRLLRTRLTMVFQHFN
                     LWSHMTVLENVMEAPIQVLGLSKQEARERAVKYLAKVGIDERAQGKYPVHLSGGQQQR
                     VSIARALAMEPEVLLFDEPTSALDPELVGEVLRIMQQLAEEGKTMVVVTHEMGFARHV
                     STHVIFLHQGKIEEEGAPEQLFGNPQSPRLQQFLKGSLK"
     gene            complement(17374..18090)
                     /gene="hisM"
                     /locus_tag="ACHKBL_000929"
     CDS             complement(17374..18090)
                     /gene="hisM"
                     /locus_tag="ACHKBL_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO5817842.1"
                     /translation="MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFL
                     AIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYSGMYTLEIVKGTEFLNAFFRSGL
                     NCTVLALTLNTCAYTTEIFAGAIRSVPHGEIEAARAYGFSTFKMYRCIILPSALRIAL
                     PAYSNEVILMLHSTALAFTATVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLI
                     SLFRRAEKRWLQHVKPSSTH"
     gene            complement(18087..18773)
                     /gene="hisQ"
                     /locus_tag="ACHKBL_000930"
     CDS             complement(18087..18773)
                     /gene="hisQ"
                     /locus_tag="ACHKBL_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012124147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter permease HisQ"
                     /protein_id="HFO5817843.1"
                     /translation="MLYGFSGVILQGALVTLELAISSVVLAVIIGLIGAGGKLSQNRL
                     SGLIFEGYTTLIRGVPDLVLMLLIFYGLQIALNTVTEAMGVGQIDIDPMVAGIITLGF
                     IYGAYFTETFRGAFMAVPKGHIEAATAFGFTRGQVFRRIMFPAMMRYALPGIGNNWQV
                     ILKSTALVSLLGLEDVVKATQLAGKSTWEPFYFAIVCGVIYLVFTTVSNGVLLFLERR
                     YSVGVKRADL"
     gene            complement(18863..19645)
                     /gene="hisJ"
                     /locus_tag="ACHKBL_000931"
     CDS             complement(18863..19645)
                     /gene="hisJ"
                     /locus_tag="ACHKBL_000931"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter substrate-binding
                     protein HisJ"
                     /protein_id="HFO5817844.1"
                     /translation="MKKLVLSLSLVLAFSSATAAFAAIPQNIRIGTDPTYAPFESKNS
                     QGELVGFDIDLAKELCKRINTQCTFVENPLDALIPSLKAKKIDAIMSSLSITEKRQQE
                     IAFTDKLYAADSRLVVAKNSDIQPTVESLKGKRVGVLQGTTQETFGNEHWAPKGIEIV
                     SYQGQDNIYSDLTAGRIDAAFQDEVAASEGFLKQPVGKDYKFGGPSVKDEKLFGVGTG
                     MGLRKEDNELREALNKAFAEMRADGTYEKLAKKYFDFDVYGG"
     gene            complement(19866..20648)
                     /gene="argT"
                     /locus_tag="ACHKBL_000932"
     CDS             complement(19866..20648)
                     /gene="argT"
                     /locus_tag="ACHKBL_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine/arginine/ornithine ABC transporter
                     substrate-binding protein ArgT"
                     /protein_id="HFO5817845.1"
                     /translation="MKKSILALSLLVGLSAVASSYAALPETVRIGTDTTYAPFSSKDA
                     KGDFVGFDIDLGNEMCKRMQVKCTWVASDFDALIPSLKAKKIDAIISSLSITDKRQQE
                     IAFSDKLYAADSRLIAAKGSPIQPTLDSLKGKHVGVLQGSTQEAYANETWRSKGVDVV
                     AYANQDLVYSDLAAGRLDAALQDEVAASEGFLKQPAGKDFAFAGSSVKDKKYFGDGTG
                     VGLRKDDAELTAAFNKALGELRQDGTYDKMAKKYFDFNVYGD"
     gene            complement(20914..21483)
                     /gene="ubiX"
                     /locus_tag="ACHKBL_000933"
     CDS             complement(20914..21483)
                     /gene="ubiX"
                     /locus_tag="ACHKBL_000933"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HFO5817846.1"
                     /translation="MKRLIVGISGASGAIYGVRLLQVLRDVTDIETHLVMSQAARQTL
                     SLETDFSLREVQALADVTHDARDIAASISSGSFQTLGMVILPCSIKTLSGIVHSYTDG
                     LLTRAADVVLKERRPLVLCVRETPLHLGHLRLMTQAAEIGAVIMPPVPAFYHRPQSLD
                     DVINQTVNRVLDQFAITLPEDLFARWQGA"
     gene            complement(21578..23095)
                     /gene="purF"
                     /locus_tag="ACHKBL_000934"
     CDS             complement(21578..23095)
                     /gene="purF"
                     /locus_tag="ACHKBL_000934"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HFO5817847.1"
                     /translation="MCGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFR
                     LRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPYGITLAHN
                     GNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAAT
                     NRLIRGAYACVAMIIGHGMVAFRDPNGIRPLVLGKRDIDENRTEYMVASESVALDTLG
                     FDFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSAR
                     VNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRT
                     FIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVY
                     LASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGLIFQDLNDLIEAVRAE
                     NPDIQQFECSVFNGVYVTKDVDQGYLDFLDTLRNDDAKAVQRQNEVENLEMHNEG"
     gene            complement(23132..23620)
                     /gene="cvpA"
                     /locus_tag="ACHKBL_000935"
     CDS             complement(23132..23620)
                     /gene="cvpA"
                     /locus_tag="ACHKBL_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V production protein"
                     /protein_id="HFO5817848.1"
                     /translation="MVWIDYAIIAVIAFSSLVSLIRGFVREALSLVTWGCAFFVASHY
                     YTYLSVWFTGFEDELVRNGIAIAVLFIATLIVGAIVNFVIGQLVEKTGLSGTDRVLGV
                     CFGALRGVLIVAAILFFLDSFTGVSKSEDWSKSQLIPQFSFIIRWFFDYLQSSSSFLP
                     RA"
     gene            complement(23879..24529)
                     /gene="dedD"
                     /locus_tag="ACHKBL_000936"
     CDS             complement(23879..24529)
                     /gene="dedD"
                     /locus_tag="ACHKBL_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030428 - cell septum [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_process: GO:0032506 - cytokinetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DedD"
                     /protein_id="HFO5817849.1"
                     /translation="MASKFQNRLVGTIVLVALGVIVLPGLLDGQKKHYQDEFAAIPLV
                     PKAGDRDEPDMMPAATQALPTQPPEGAAEEVRAGDAAAPSLDPATIAVNNTEFEPEPA
                     PVAPPKPKPVESPKPKVEAPPASKPVVEEKAAPTGKAYVVQLGALKNADKVNEIVGKL
                     RGAGYRVYTSPSTPVQGKITRILVGPDASKDKLKGSLGELKQLSGLSGVVMGYTPN"
     gene            complement(24519..25787)
                     /gene="folC"
                     /locus_tag="ACHKBL_000937"
     CDS             complement(24519..25787)
                     /gene="folC"
                     /locus_tag="ACHKBL_000937"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HFO5817850.1"
                     /translation="MIIKRTPQAASPLASWLSYLENLHSKTIDLGLERVSQVAARLGV
                     LKPAPFVFTVAGTNGKGTTCRTLESILMAAGYKVGVYTSPHLVRYTERVRVQGQELPE
                     SAHTASFAEIESARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVD
                     ADVAVVTSIALDHTDWLGPDRESIGREKAGIFRSEKPAIVGEPEMPSTIADVAQEKSA
                     LLQRRGVEWNYSVTDHDWAFSDAHGTLENLPLPLVPQPNAATALAALRASGLEVSENA
                     IRDGIASAILPGRFQIVSESPRVIFDVAHNPHAAEYLTGRMKALPKNGRVLAVIGMLH
                     DKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGNGKSFDSVAQAWDAAMADA
                     KAEDTVLVCGSFHTVAHVMEVIDARRSGGK"
     gene            complement(25857..26771)
                     /gene="accD"
                     /locus_tag="ACHKBL_000938"
     CDS             complement(25857..26771)
                     /gene="accD"
                     /locus_tag="ACHKBL_000938"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HFO5817851.1"
                     /translation="MSWIERIKSNITPTRKASIPEGVWTKCDSCGQVLYRAELERNLE
                     VCPKCDHHMRMTARNRLHSLLDEGSLVELGSELEPKDVLKFRDSKKYKDRLASAQKET
                     GEKDALVVMKGTLYGMPVVAAAFEFAFMGGSMGSVVGARFVRAVEQALEDNCPLICFS
                     ASGGARMQEALMSLMQMAKTSAALAKMQERGLPYISVLTDPTMGGVSASFAMLGDLNI
                     AEPKALIGFAGPRVIEQTVREKLPPGFQRSEFLIEKGAIDMIVRRPEMRLKLASILAK
                     LMNLPAPNPEAPREGVVVPSVPDQEPEA"
     gene            complement(26927..27586)
                     /gene="dedA"
                     /locus_tag="ACHKBL_000939"
     CDS             complement(26927..27586)
                     /gene="dedA"
                     /locus_tag="ACHKBL_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFO5817852.1"
                     /translation="MDLIYFLIDFILHIDVHLAELVAEYGVWVYAILFLILFCETGLV
                     VTPFLPGDSLLFVAGALASLETNDLNVHMMVVLMLIAAIVGDAVNYTIGRLFGDKLFS
                     NPDSKIFRRSYLDKTHQFYEKHGGKTIILARFVPIVRTFAPFVAGMGHMSYRHFAAFN
                     VIGALLWVLIFTYAGYFFGTLPFIQSNLKLMIVGIIFVSILPGVFEFIRHKRAAARAA
                     K"
     gene            complement(27669..28481)
                     /gene="truA"
                     /locus_tag="ACHKBL_000940"
     CDS             complement(27669..28481)
                     /gene="truA"
                     /locus_tag="ACHKBL_000940"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFO5817853.1"
                     /translation="MSDQQQPPVYKIALGIEYDGSRYYGWQRQNEVRSVQEKLEKALS
                     QVANEPITVFCAGRTDAGVHGTGQVVHFETTAQRKDAAWTLGVNANLPGDIAVRWVKA
                     VPDDFHARFSATARRYRYIIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLLGEND
                     FTSFRAVQCQSRTPWRNVMHINVTRHGPYVVVDIKANAFVHHMVRNIVGSLMEVGAHN
                     QPESWIAELLAAKDRTLAAATAKAEGLYLVAVDYPDRYDLPKPPMGPLFLAD"
     gene            complement(28481..29494)
                     /gene="usg"
                     /locus_tag="ACHKBL_000941"
     CDS             complement(28481..29494)
                     /gene="usg"
                     /locus_tag="ACHKBL_000941"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFO5817854.1"
                     /translation="MSEGWNIAVLGATGAVGEALLETLAERQFPVGEIYALARNESAG
                     EQLRFGGKTITVQDAAEFDWTQAQLAFFVAGKEATAAWVEEATNSGCLVIDSSGLFAL
                     EPDVPLVVPEVNPFVLTDYRNRNVIAVPDSLTSQLLAALKPLIDQGGLSRISVTSLIS
                     ASAQGKKAVDALAGQSAKLLNGIPIDEEDFFGRQLAFNMLPLLPDSEGSVREERRIVD
                     EVRKILQDEGLMISASVVQAPVFYGHAQMVNFEALRPLAAEEARDAFAQGEDIVLSEE
                     NEFPTQVGDASGTPHLSVGCVRNDYGMPEQVQFWSVADNVRFGGALMAVKIAEKLVQE
                     YLY"
     gene            complement(29560..30717)
                     /gene="pdxB"
                     /locus_tag="ACHKBL_000942"
     CDS             complement(29560..30717)
                     /gene="pdxB"
                     /locus_tag="ACHKBL_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020218743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033711 - 4-phosphoerythronate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HFO5817855.1"
                     /translation="MSRTEKLLTILVDENMPYARDLFSRLGEVTAVPGRPIPVAQLAD
                     ADALMVRSVTKVNESLLAGKPIKFVGTATAGTDHVDDTWLKQAGIGFSAAPGCNAIAV
                     VEYVFSSLLMLAERDGFSLHERTVGIVGVGNVGRRLQARLEALGITTLLCDPPRADRG
                     DEGDFRSLDELVQHADILTFHTPLFKDGPYKTRHLADEKLIRSLKPGAILINACRGAV
                     VDNTALLTCLNEGQKLSVVLDVWEGEPELNVELLTKVDIGTPHIAGYTLEGKARGTTQ
                     VFEAYSKFIGHEQHVALDTLLPAPEFGRITLHGPLDQPTLKRLVHLVYDVRRDDAPLR
                     KVAGIPGEFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR"
     gene            complement(30877..31881)
                     /locus_tag="ACHKBL_000943"
     CDS             complement(30877..31881)
                     /locus_tag="ACHKBL_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001709053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO5817856.1"
                     /translation="MSIKKLEDGRYLLDIRPNGRKGKRVRKVFNKKSVAVATERYIMA
                     NAEKREYIQGYRDRRTLNDLLELWWVYHGQHRRKAEEDRKQLRNIINELGADMQAVDL
                     DKLKIIAWRSRRIADGLKPSSANRYMNRLSGMFTALKKIGLWDAEHPVRGISVLYVSP
                     REMAFLSQKEVALLLDTLEGDYWRVALLCLSTGARWGEASKLRGEQIVHNRVTFLETK
                     NGRKRTVPISQAVCEAIKTSETGGLFDVKYREFCLVLKRVKPDLPKGQAAHVLRHTFA
                     SHFVMNGGNIIALQRILGHATIQQTMAYAHFAPDYLQDAVALNPLKGGVSVHSVSTGE
                     "
     gene            complement(31978..32298)
                     /locus_tag="ACHKBL_000944"
     CDS             complement(31978..32298)
                     /locus_tag="ACHKBL_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001390705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO5817857.1"
                     /translation="MASLSSMGEKFRLIREAEGMTRQEFADCVGLPYGTVTNYEVRGK
                     QVTEGALLKVTKHPKFKKYAYWLSTDETMPEAGQIAPALSLDGSCNLEGNQDSTETTR
                     KLPR"
     gene            32412..32699
                     /locus_tag="ACHKBL_000945"
     CDS             32412..32699
                     /locus_tag="ACHKBL_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cox family DNA-binding protein"
                     /protein_id="HFO5817858.1"
                     /translation="MRESELEGFIQVAPYPLEAVPYQLFAKMIGRKESTVRTMIDAAK
                     LPTIDFVKPGSVKTRASENWVYLPAFNAGMRKAFFNQPKEQRDAWLLWLGL"
     gene            32895..33233
                     /locus_tag="ACHKBL_000946"
     CDS             32895..33233
                     /locus_tag="ACHKBL_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000194243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4761 family protein"
                     /protein_id="HFO5817859.1"
                     /translation="MTQRRSFRTGTERHAARFSTSASRSNTHYSLQETHATPDGHLVK
                     QTGKHTWLINDAGIVIHRGARNPITGNTCYSLTRGDEQFGQDFTLHEALRTADRLISG
                     RSFQHPEHRH"
     gene            33253..>33482
                     /locus_tag="ACHKBL_000947"
     CDS             33253..>33482
                     /locus_tag="ACHKBL_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4754 family protein"
                     /protein_id="HFO5817860.1"
                     /translation="MIEAYKILINNALQRYHFELANRGVTRDEGLRNSLRGAIMGLYN
                     CALGSDDREALEELKVIVAELFEGEIVEPYFT"
BASE COUNT         8404 a         9101 c         8062 g         7915 t
ORIGIN      
        1 gcatcatcct catacattaa tcccgcttaa caatagtttc acaccttgca cccattacga
       61 cacctcggaa atcatcacgc tgttaaactc tccgtaatgc ggaaatatca tcatcacgat
      121 tcattagtga attcttaaac gaggcatttt caaacaatta tcttacagat aaaaaaacca
      181 cagtcacaat taagaatcag aacaattcac tacataacat taattactaa gcatatacac
      241 ctcattattt tgccattatt aagttattaa cagcacaatc gagtcttccc ctctggcaaa
      301 atcttattct gcagaccttc aaaacgccgt cctgggggag tacattgttc taagctgact
      361 tccacggcag ggagtggcga taacagcaaa aaaggtcaag attcatgtcc cccattgaaa
      421 aatccagcaa attagagaat gtctgttacg acatccgtgg tccggtgctg aaagaagcaa
      481 aacgcctgga agaagaaggt aacaaggtac tgaaactgaa catcggcaac cctgccccgt
      541 tcggttttga cgcgccagat gaaatcctcg ttgacgtgat tcgcaacctg cctaccgctc
      601 aagggtattg cgattccaaa gggctttact ccgcgcgtaa agccatcatg cagcactacc
      661 aggctcgtgg catgcgtgat gttaccgtgg aagatattta catcggaaat ggtgtatcgg
      721 agcttatcgt tcaggcaatg caggcattgc tgaacagcgg cgacgaaatg ttggttcctg
      781 cacctgatta cccactatgg accgcggcgg tttcgctctc cagcggtaaa gcggtgcatt
      841 atctttgcga tgaatcctct gactggttcc cggaccttga tgatattcgc gctaaaatta
      901 cgcctcgtac gcgtgggatc gttattatca acccaaataa cccaaccggc gcggtatatt
      961 ccaaagagct tttaatggag atagtggaga ttgcacgtca gcataatctc attatcttcg
     1021 ccgatgaaat ttatgacaaa attctctacg acgacgctga gcatcactca attgcgccgc
     1081 tggcacctga tctgctgacc attaccttta acggcctgtc gaaaacatac cgcgttgcag
     1141 gcttccgtca ggggtggatg gtgttgaacg ggccgaaaaa acacgccaaa ggctacatcg
     1201 aaggtctgga aatgctggct tcaatgcgcc tgtgtgctaa cgttcctgcg caacacgcca
     1261 ttcagaccgc gctgggcggt tatcagagca tcagtgaatt tattacccct ggcggtcgtc
     1321 tttatgagca gcgtaaccgc gcatgggaat tgatcaatga tattccgggc gtttcctgcg
     1381 tgaaacctcg tggtgcgctg tacatgttcc cgaaaatcga cgccaaacgc tttaatattc
     1441 acgacgatca gaaaatggtg ctggatttcc tgttgcagga aaaagttctg ttggtgcaag
     1501 ggacggcatt caactggccg tggccggatc acttccgcat tgtcacgcta ccgcgtgtcg
     1561 atgatatcga gctgtctttg agcaagttcg cgcgtttcct ttctggttat catcagctgt
     1621 aatcttaatt tcactgccgg agattgcatc cggcagtgtt atcccgccac aatgacctga
     1681 tgatgtcatc atacgtaagg tcactatgaa acagagccat ttttttgccc atctctcccg
     1741 cctgaaactc attaaccgct ggccgctaat gcgcaatgtg cggacggaaa atgtgtccga
     1801 acacagtttg caggtagcga tggtcgccca tgcgctggca gctatcaaaa atcgtaaatt
     1861 tggcggtaat gtcaacgccg aacgtatcgc tttactggcg atgtaccacg atgcctcaga
     1921 agtgcttacc ggcgatctcc ctaccccggt gaaatacttc aattcgcaaa tcgctcagga
     1981 atacaaggcg attgaaaaaa tcgctcagca aaaactggtc gatatggttc cggaagagtt
     2041 gcgggatatc tttgcgccgt taattgacga gcatgcatat agtgatgaag aaaaatcact
     2101 ggtgaaacag gcagatgcac tatgtgcata tctgaaatgt ctggaagaac tcgcggcggg
     2161 aaataatgaa ttcttgctgg caaaaacgcg actggaagcg acgcttgaag cgcgccgcag
     2221 ccaggagatg gactacttca tggaagtctt tgttcccagc ttccatcttt cgctcgatga
     2281 gattagccag gattcaccgc tgtaagcagc cggagtctgc gtcgcatcag gcaataagcg
     2341 ccggatgcga catcaggctc ttgtcaaaac ggaaacagca tcgggatcat caccacacaa
     2401 accgccatca cgataatggt gaacggtacc cccaacttca caaagtcact gaagctgtaa
     2461 tttcccggac ctaaaaccag tgtgttaaca ggtgaagaaa ccggcgtcat aaaggcggcg
     2521 gatgctgcca tcgccacgac catcgcgaat gggtaaggcg acacccccat cgttttagca
     2581 gcagccagcg caatcggagc catcaacacc gccgtcgcgg tattagaaat aaatagccca
     2641 ataaccgccg acaagacaaa caaacaaccc agcatcatat gtggcccgta accgccgcca
     2701 atgtccatca gccctttcac cgccagcgca acacctcccg ttttctgtaa tgccacagca
     2761 aacggcatca tcccaacgat caaaataatg ctcggccagt gtatggattt ataggcgctt
     2821 tcagcatcga tacagcggaa tttccccatc agcaggcagg cgatgatagc ggcaacagga
     2881 ttaggaattt catcggtcag cattaacgcc accattagca ccagacagaa aatggcatgg
     2941 ggtgcctgac tgtgcgcggg cgatgcttca ctcacctctt ccggtaagtt cagcgctacg
     3001 aagtcgcggc cctgtttggc cagcataccg atcagtttcc agttacccac aaccaggatg
     3061 atatcgccca gcagcagagg ctcatccgcc agcgaacctt ccagcgccac gccattgcgc
     3121 tttagcccca ctacattcaa tccgtagcgg gtacgaaaac caatttcgcg caccgattta
     3181 ccaataagtt ctgactcagg aattaatgaa atctctgcca tgcccacatc aagggcctgg
     3241 tcagaaaaat actcgccgcg cagtaccatc ggctccagca attgctcact acaaaattgc
     3301 cggagatcga catcagccgc agacatatca ataagcaaaa cgtcacgcgc gcgaaattca
     3361 gaaaccccat taacgttcac gataacgcga cgaaaacgcc gccagcgttc aacaccgatg
     3421 acgttagcac cataacgttc acgtaatttg agatcgtcca gtcgttgacc aatcattggc
     3481 gatccggggc gaatagccag acgtcgcgca cgcccggtca gtcgatattc acggataaga
     3541 tcgcgaaagg ttcggcgcgt ccagccttcg cgttgtgggg tctgggtatc ccctttcagc
     3601 atgaaacgca tcactaacat atacaaaata cccagcacca gcacgaccag gccaataggt
     3661 gttacgctaa agaaactgaa gccgtgatag ccttcacgca gcaattcact gttgactacc
     3721 aggttcggcg gcgtcgccac cagcgtcatc atgccgctta tcagcccggc aaaacttaac
     3781 ggcatcatca gacgcgacgg cgacgtttgc atacgcatgg caacgcttaa caccacggga
     3841 ataaagatag cgacaacgcc ggttgaactc ataaacgcgc caagccccgc gacggtcagc
     3901 atcaacaaaa ccaacatttt gatttcactg ttgcccgcca ctttgaccag ccatgtcccc
     3961 attacagtgg caacaccggt acggaccaaa ccatcgccaa taatgaacaa ggcggcaatc
     4021 aggacaacgt taggatcgga aaagccggaa aatacttcgg ggaccgtcag cgttccgctt
     4081 aatgcaaacg cgacaataac aaacaaagcg accgcatcca tacgcactct gcccgtcgca
     4141 aacaagacga tggcaacggc cagtaatgaa agaacccaaa tcaattcacc gttcacaact
     4201 tatccttgtt aattgagggg gataacttga ttctgccata aaaaagcccc gacgagacgg
     4261 ggctaaatca tgatcaggtg tttcactgaa taataacatc gccatttggc tgtttggtca
     4321 cagtaatttg ctccagactg tggaggacca aatcgacctc attcaggcgc ggggtatctg
     4381 ccggagcgtt aaccgcaatg acatggcaac ccgccgccag gccagaaagc acgccagcgg
     4441 gagcatcttc caccactaca cactcctgcg gcgcaagccc cagcagctgc gcgcctagca
     4501 gatacgcatc aggttctggt tttccgcgct tcactcgctc agcggtcaca aacacctctg
     4561 gcgcgggaag cccagctatt ttatggcgcg ctcgcgctac cggcatggag ccagaagtca
     4621 caatggccca cggaatacct gctttattca aatgattgag taaggcgatt gcccccggaa
     4681 gtgcggtaat accctcggtt tccgtggcct cgatttgctc cagacgcgta aactcggcgg
     4741 caatatcagc ctcggatttg cccgccataa aatggcgcaa agaggtgatc gcctgtttac
     4801 cgtgaataaa agccagcacc tcttccggcg ctaacccatg acgtctggcc cagttgctcc
     4861 acgcccgttc taccgcaggc agggaatcca ccagcgttcc atcaagatca aacagaaaac
     4921 ctttgcaccg cacgcgggcc tcctcaggcg ttgataattt gattaatttc gttggcgctc
     4981 aaatggtact gacgcgggca ggcatgccac acattaagca tgcgctgata tttttcccac
     5041 attggcgtct gggcgttaaa accgtgagtt ccggcatcaa aatgggtata gcgtccttcc
     5101 acattaacca taaagcggac ataaccgagg taacgtgctt cagtggcggc gtcaaagccg
     5161 aggaaggtga cacgacgttc atcgatggat tgctggtcct gcaaattaga ccaggaaaca
     5221 tgcaacgcat gatacatctc cataatgtcg atgatagtgc ggcaggtttc ttctttcagc
     5281 tcgccaaact cgcgatccag ttcacgcatc tgtaatccat aaccacgctc aatgattgtt
     5341 tgcaggcgac ggtaacgttc agcatttgcc ggatcgagca tagtcatcat cttgtactgg
     5401 ttagacaaaa taagacgttg cgcgttggtc atttccattg ttgactcctg tatcactcta
     5461 ctacggtgaa aaaaaaagaa ggctgagtat gccttctttt atatgcgtaa tcaggggtca
     5521 attacaaatc atcaaggaaa gttttatcca gttgtttgaa ggcgcgctta agcgtgtcag
     5581 ctaatgcctg gtaatcaggc ttgccttcaa cgggtgccaa cacctgtcca gactcctgca
     5641 atttaccgcg aacttcataa aaccagttaa ggattgcagg gggtaatggc gttacagaac
     5701 gtttgcccag ccaccacaat ccctgcatgg gtaaacttaa ggcgaacagc gcagtggcaa
     5761 ctgccggccc aagctgaccg cccagggcaa tctgccagca gagagtaaat acggcgatcg
     5821 gcggcataaa acggatcgca taacgcgtca tcttgataac gcgattttcg acaaagaccg
     5881 gggcaaggcg tttttccagc ggccacgtct ttgagtaatg ctgtccccgg cgaaacaagc
     5941 taaaaaaatt aacagaacga ttatccggcg ttgacatgct tcacctcaac ttcacatata
     6001 aagattcaaa aatttgtgca aattcacaac tcagcgggac aacgttcaaa acattttgtc
     6061 ttccataccc actatcaggt atcctttagc agcctgaagg cctaagtagt acatattcat
     6121 tgagtcgtca aattcatata cattatgcca ttggctgaaa attacgcaaa atggcataga
     6181 ctcaagatat ttcttccatc atgcaaaaaa aatttgcagt gcatgatgtt aatcataaat
     6241 gtcggtgtca tcatgcgcta cgctctatgg ctccctgacg tttttttagc cacgtatcaa
     6301 ttataggtac ttccatgtcg agtaagttag tactggttct gaactgcggt agttcttcac
     6361 tgaaatttgc catcatcgat gcagtaaatg gtgaagagta cctttctggt ttagccgaat
     6421 gtttccacct gcccgaagca cgtatcaaat ggaaaatgga cggcaataaa caggaagcgg
     6481 ctttaggtgc aggcgccgct cacagcgaag cgctcaactt tatcgttaat actattctgg
     6541 cacaaaaacc agaactgtct gcgcagctga ctgctatcgg tcaccgtatc gtacacggcg
     6601 gcgaaaagta taccagctcc gtagtgatcg atgagtctgt tattcagggt atcaaagatg
     6661 cagcttcttt tgcaccgctg cacaacccgg ctcacctgat cggtatcgaa gaagctctga
     6721 aatctttccc acagctgaaa gacaaaaacg ttgctgtatt tgacaccgcg ttccaccaga
     6781 ctatgccgga agagtcttac ctctacgccc tgccgtacaa cctgtacaaa gagcacggca
     6841 tccgtcgtta cggcgcgcac ggcaccagcc acttctatgt aacccaggaa gcggcaaaaa
     6901 tgctgaacaa accggtagaa gaactgaaca tcatcacctg ccacctgggc aacggtggtt
     6961 ccgtttctgc tatccgcaac ggtaaatgcg ttgacacctc tatgggcctg accccgctgg
     7021 aaggtctggt catgggtacc cgttctggtg atatcgatcc ggcgatcatc ttccacctgc
     7081 acgacaccct gggcatgagc gttgacgcaa tcaacaaact gctgaccaaa gagtctggcc
     7141 tgctgggtct gaccgaagtg accagcgact gccgctatgt tgaagacaac tacgcgacga
     7201 aagaagacgc gaagcgcgca atggacgttt actgccaccg cctggcgaaa tacatcggtg
     7261 cctacactgc gctgatggat ggtcgtctgg acgctgttgt attcaccggt ggtatcggtg
     7321 aaaatgccgc gatggttcgt gaactgtctc tgggcaaact gggcgtgctg ggctttgaag
     7381 ttgatcatga acgcaacctg gctgcacgtt tcggcaaatc tggtttcatc aacaaagaag
     7441 gtacccgtcc tgcggtggtt atcccaacca acgaagaact ggttatcgcg caagacgcga
     7501 gccgcctgac tgcctgattt cacaccgcca gctcagctgg cggtgctgtt ttgtaacccg
     7561 ccaaatcggc ggtaacgaaa gaggataaac cgtgtcccgt attattatgc tgatccctac
     7621 cggaaccagc gtcggtctga ccagcgtcag ccttggcgtg atccgtgcaa tggaacgcaa
     7681 aggcgttcgt ctgagcgttt tcaaacctat cgctcagccg cgtaccggtg gcgatgcgcc
     7741 cgatcagact acgactatcg tgcgtgcgaa ctcttccacc acgacggccg ctgaaccgct
     7801 gaaaatgagc tacgttgaag gtctgctttc cagcaatcag aaagatgtgc tgatggaaga
     7861 gatcgtcgca aactaccacg ctaacaccaa agacgctgaa gtcgttctgg ttgaaggtct
     7921 ggtcccgaca cgtaagcacc agtttgccca gtctctgaac tacgaaatcg ctaaaacgct
     7981 gaatgcggaa atcgtcttcg ttatgtctca gggcactgac accccggaac agctgaaaga
     8041 gcgtattgaa ctgacccgca acagcttcgg cggtgccaaa aacaccaaca tcaccggcgt
     8101 tatcgttaac aaactgaatg caccggttga tgaacagggt cgtactcgcc cggatctgtc
     8161 cgagattttc gacgactctt ccaaagctaa agtaaacaat gttgatccgg cgaagctgca
     8221 agaatccagc ccgctgccgg ttctcggcgc ggtgccgtgg agctttgacc tgatcgcgac
     8281 tcgtgcgatc gatatggctc gccacctgaa tgcaaccatc atcaacgaag gcgacatcaa
     8341 tactcgccgc gttaaatccg tcactttctg cgcacgcagc attccacaca tgctggagca
     8401 cttccgtgcc ggttctctgc tggtgacttc cgcagaccgt cctgacgtgc tggttgccgc
     8461 gtgcctggct gccatgaacg gcgtagaaat cggtgccctg ctgctgactg gcggctacga
     8521 aatggacgcg cgcatttcta aactgtgcga acgtgctttc gctaccggcc tgccggtatt
     8581 tatggtgaac accaacacct ggcagacttc tctgagcctg cagagcttca acctggaagt
     8641 tccggttgac gatcacgagc gtatcgagaa agttcaggaa tacgttgcta actacatcaa
     8701 cgctgactgg atcgattctc tgactgccac ttctgagcgc agccgtcgtc tgtctccgcc
     8761 agcgttccgt tatcagctga ctgaacttgc gcgcaaagcg ggcaaacgta tcgttctgcc
     8821 ggaaggtgac gaaccgcgta ccgttaaggc agccgctatc tgtgctgaac gtggtatcgc
     8881 aacttgcgta ctgctgggta atccggcaga gatcaaccgt gttgcagcgt ctcagggtgt
     8941 agaactgggt gcaggcattg aaatcgttga tccagaagtg gttcgcgaaa gctatgttgg
     9001 tcgtctggtc gagctgcgta agaacaaagg catgaccgaa accgttgccc gcgaacagct
     9061 ggaagataac gtggttctcg gtacgctgat gctggaacag gatgaagttg atggtctggt
     9121 ttccggtgct gttcacacta ccgcaaacac catccgtccg ccgctgcagc tgatcaaaac
     9181 tgcaccgggc agctccctgg tatcttccgt gttcttcatg ctgctgccgg aacaggttta
     9241 cgtttacggt gactgtgcga tcaacccgga tccgaccgca gaacagctgg cagaaatcgc
     9301 gattcagtcc gcagattccg ctgcggcctt cggtatcgaa ccgcgcgttg ctatgctctc
     9361 ctactccacc gggacttctg gtgctggtag cgacgtagaa aaagttcgcg aagcaactcg
     9421 tctggcgcag gaaaaacgtc ctgacctgat gatcgacggt ccgctgcagt acgacgctgc
     9481 ggtaatggct gacgttgcga aatccaaagc accgaactct ccggttgcag gtcgcgctac
     9541 cgtgttcatc ttcccggatc tgaacaccgg taacaccacc tacaaagcgg tacagcgttc
     9601 tgctgacctg atctctatcg gaccgatgct gcagggtatg cgcaagccgg ttaacgacct
     9661 gtcccgtggc gcactggttg acgatatcgt ctacaccatc gcgctgactg cgattcagtc
     9721 tgcacagcag cagtaatctc gtcatcatcc gcagctttac gctgcggata tctgaaccgg
     9781 aaatagtcac tatttccggt tttttattct cttaatttgc attaatcctt tctgattatc
     9841 ttgcttaact gcgctgcatc aatgaattgc gccatctcaa tttgcatact taccactttg
     9901 ttttgtgcaa gggaatattt gcgctatgtc cgcaatcact gaatctaaac caacaagaag
     9961 atgggcaatg cccgatacgt tggtgattat cttttttgtt gccattttaa ccagccttgc
    10021 cacctgggta gttccggtgg gcatgtttga cagtcaggaa gtgcagtatc aggttgatgg
    10081 tcaaacaaaa acacgcaaag tcgtagatcc acactcattt cgcattctga ctaacgaagc
    10141 aggcgaacct gagtatcacc gcgtacagct gttcacgacg ggcgatgaac gcccgggtct
    10201 gatgaacttc ccgtttgaag ggttaacctc aggatcgaaa tacgggacag ccgttggcat
    10261 catcatgttt atgctggtga ttggcggcgc gtttggcatt gtgatgcgta caggaaccat
    10321 tgataacggt atcctggcgc ttattcgcca tacccgcgga aatgaaattc tctttattcc
    10381 tgcgctgttt attctgtttt cacttggcgg cgcaatattt ggtatgggag aagaggccgt
    10441 cgcctttgcc attattatcg caccgctaat ggtccggctg ggctatgaca gtattaccac
    10501 cgtcctggtg acctatatcg ccacgcaaat cggttttgcc agttcgtgga tgaacccgtt
    10561 ttgtgtggtt gttgctcagg ggattgccgg cgttccggtg ctttctggct ccgggttgcg
    10621 catcgtggtg tgggttatcg ccaccctgat tggcctgatc tttaccatgg tgtacgcctc
    10681 acgagtgaaa aagaatcctc ttctgtcacg cgtgcatgag tccgaccgct tctttcgtga
    10741 aaagcaggcg gatgttgaac aacgtccgtt tacctttggt gactggctgg tattgattgt
    10801 cctgaccgcc gtaatggtct gggtgatttg gggcgtgatc gttaatgcct ggtttattcc
    10861 agaaattgcc agccagttct tcaccatggg tctggtgatt ggcatcatcg gcgttgtttt
    10921 ccgccttaac ggcatgacgg ttaataccat ggcttcatcc tttaccgaag gggcgcgaat
    10981 gatgatcgcc cctgccctgc tggtgggttt cgccaaaggg attttgctgc tggtcggtaa
    11041 tggtgaagcg ggtgatgcca gcgtgttaaa taccatcctc aacagcattg ccaatgccat
    11101 tagcggtctg gataacgcgg tcgcggcctg gtttatgttg ctcttccagg cggtatttaa
    11161 tttcttcgtg acatctggtt ctggtcaggc ggcgttaacc atgccgttac tggcaccgct
    11221 tggcgatctg gtcggtgtaa accgtcaggt taccgttctg gcgttccagt ttggtgatgg
    11281 cttcagccac atcatttacc cgacctcagc ttcgttaatg gcaacgctcg gtgtttgccg
    11341 ggtggacttc cgtaactggc tgaaggtggg cgcgacactg cttggactgc tgtttattat
    11401 gtccagcgtc gtggtgatcg gcgctcagtt gatgggctac cactaaaaat gttaagagcc
    11461 gcaattgcgg ctcttttttc attctgccgt ttcagtctct acggcttcat ttttggcatt
    11521 gcgcttcatc cacaacgcca gcgctttcag cgagtctgga gtgaactcat cgcagcgtgc
    11581 ggtgatttct tccggcgtca gccagcaaac ttcgctgact tcatcttcct gtagggcgaa
    11641 gggaccgtga gagacgcagc tgaacaacgc gccccagaca cggcaatttt tatcttcgaa
    11701 atagaactgc ccgtgctcgg caaaggggac accggcaatg cccaactctt cttccgcttc
    11761 gcgacgcgcg gattccagca gttgctcatc ggcctggact acaccgcctg cggtcgcatc
    11821 taacatgccg ggtaaaaagt cttttgtctc ggtacgacgc tggaccagaa ttttgcccat
    11881 gccatcatgc acgacgatgt aagttgcacg atgacgcaga cactgtgccc gcatttgttc
    11941 ccggctggct tgtgcaatga cttcgttctc ttcattgaca atatccaccc attcagtact
    12001 tgccaaacga cgctgttcca ttatcaggaa accttctttt tctggcgctc ttacggcgca
    12061 ttttgagttg tgggtaaatt acggattaat cgcgacctgc gcaatgatac tttgatcatt
    12121 gagtgcgatt acactaagta cgtcattatc cagcatgcca taactcgccg gattaccgcc
    12181 tttaggaata ctcaccgagc cggggttgaa gtggaaaatc tccccccgct gttctgccac
    12241 tggcagatgg gtatgaccgt acaccagcac atcgttctgg tttaaagcag gtaggttttc
    12301 cgggccaaac agatgaccat gcgtcaaaaa cagacgttgt ttttccagta atacctgttg
    12361 ccacggcgcg gttatcggga aatgcaaaag catttgatcc acttcgctgt cgcaattgcc
    12421 gcgcacagcg ataaccttat gtgccacttc attaagccgt tcagcgactt tggctggcgc
    12481 gtaaccctcc ggtaaagcat tacgcgggcc gtgattcaac acgtcgccaa ggatcaccag
    12541 ccactgggca ccactttggg caaacaactc cagaacacgt tccgtcgccg gtaacgaccc
    12601 atgaatgtcc gatgcaaaca tcagtttcat cactcactcc tcgtcgaaaa aacgtaagct
    12661 atgatactgg attctgtcgt tgctatcagc cagattgctt cgccgaaaga gcaataaaac
    12721 gctggacaga cgctcgctgc cactggaatg ttgcataatc caccaggcgt tctggcacct
    12781 catcgccatg tagtaccagg cggttaatca ttagcgccag atcagtatca gcaatgcacc
    12841 attcaccaaa taaattcggc tgacccagtg ctaacaaatg ttctgccatc gcgaacagtt
    12901 tctctgcact ggcttttccc tccgccgtta atggcgcttt tttcgcaccc gcaaaaacga
    12961 catctgtcga acgttcttcg cggatgggca tcagatcgct acgcagccag gcctgaatct
    13021 gtcgtgcacg cgcacgattt tctaaatcaa gcggataaat gcgttcccag gtcggtggcg
    13081 caaatcgatc ttccagatac tccgcaatgg cagaagattc actcaactca aaatcatcga
    13141 tttgtaataa cggcacacgg cgtgtctgac cgtaaccttg ccacgtcggc tgcaaatgtt
    13201 caccgctgtc gaggtcgatg gtcttgatat gaaacgacag gcctttttcc tgcaacgcca
    13261 cccaggcgga taacacataa ggggagaaaa aatgggcatc tgaccaaagc gtgatagcgg
    13321 gtttactcat tatgacctcg gcaataaagg gggctggcta tcaaactata gagccttttt
    13381 gtcactgtca cgtgacgaat actcacgcaa gtcgccgcgc tttctatact tgtggtgttc
    13441 agatgattag acaaccggag ctgcaatgat cgatctctat ttcgccccga caccaaatgg
    13501 tcacaaaatt acgctgtttc tcgaagaagc agggctggat tatcgcttga taaaggtaga
    13561 cctggggaaa ggcggtcagt ttcgcccgga atttttgcac atttcgccta acaacaaaat
    13621 tccggcaatt gttgatcatt ctccagccga tggcggcgaa ccgctaagcc tctttgagtc
    13681 tggtgccatt ttgttgtatc tggctgagaa aacaggactc tttttgagtc atgaaacgcg
    13741 tgagcgcgcc gccacattac agtggttatt ctggcaggta ggcggactgg ggccgatgct
    13801 tgggcaaaat catcatttta atcacgcagc ctcccaaacc attccttacg ctattgaacg
    13861 ttatcaggtt gaaactcagc gtctgtacca tgtactgaac aagcggctgg aaaactcgcc
    13921 ctggctgggt ggcgagaact acagcattgc ggatattgcc tgctggccgt gggttaatgc
    13981 ctggactcgc cagcgaattg acctcgcaat gtatccggca gtcaagaact ggcatgagcg
    14041 gatccgttcg cgccctgcca ccggtcaggc actgttaaaa gcacaactcg gtgatgagcg
    14101 ttcggatagt taacagaaac aggttctcgt gtattatttc atcctaagaa aaacaacgga
    14161 gaacctgcaa tggcacaacc tgccgctatt attcgtataa agaaccttcg tttacgtacg
    14221 tttatcggaa ttaaggaaga agaaattaac aaccgtcagg atattgttat caatgtgacg
    14281 attcactacc ccgccgataa agcgcgcacc agcgaagata tcaacgatgc gctgaattat
    14341 cgcaccgtga cgaaaaacat tattcagcat gtagagaata accgtttctc tttgctggaa
    14401 aaattaactc aggatgtgct cgatatcgca cgtgaacatc actgggtgac gtatgctgaa
    14461 gtggagatcg ataaactgca cgcgctgcgc tacgccgatt cggtatccat gaccttaagc
    14521 tggcagcgtt aaccgccata tcgggaggct gcatgaagat agtgatcacc ggagggacag
    14581 gattaattgg tcgccatttg attccacgtt tgctggagct gggccatcaa atcacggtaa
    14641 tgacgcgtaa cccgcagaaa gccagttctg ttctcggccc tcgggtggca ctatggcaag
    14701 ggcttgccga tcaaagcaac ctcaacggcg ttgatgcggt aatcaacctg gccggagaac
    14761 cgattgctga taaacgctgg actcacgagc aaaaagagcg tctctgccaa agccgctgga
    14821 atatcacgca aaaactggtc gatttgatta atgccagcga cacgccgccg tcggtactca
    14881 tttccggctc ggcaacgggc tattatggcg acttaggtga agtggtggtt accgaagagg
    14941 aaccgccgca taacgaattt actcataaac tctgcgccca ctgggaagaa attgcctgcc
    15001 gggcgcaaag tgacaaaacg cgagtgtgcc tgctgcgtac cggcgtagtg ctggcaccgg
    15061 atggcggtat tctcggtaaa atgctgccgc cgtttcgtct tggcctgggc gggccgattg
    15121 gttccggtcg gcagtatctg gcctggattc atattgatga tatggtcaac ggcattctct
    15181 ggctgctgga taacgagctg cgcgggccat ttaatatggt ttcgccctac ccggtacgca
    15241 atgaacaatt tgcccatgcg ctcggtcatg ctctgcatcg cccggctatt ttgcgcgtcc
    15301 ccgcaaccgc cattcggctg ttaatgggcg aatcttcagt actggtatta ggtggacaac
    15361 gcgcgctgcc taaacggctg gaagaagcgg gttttgcgtt tcgttggtac gatttagaag
    15421 aggcgctggc ggatgtcgtt cgctgatgtg gtttactgca aacatccgcc agttaactcc
    15481 cggtgttaca gggttagtgg ctttgcgcga taagatcgtc tggtgaaagt cgggtcacca
    15541 tcataactaa ctctcggtct aaacctctct ccagcatctc ctgagcaata cgcagggctt
    15601 cttcgcgttt gccaagaatt aatccatcat tccgcccttc ttcacgtaat ctgtcagcaa
    15661 tagtcatcag tttctccttt tcttgtggtg cgcgttccgc tatctcacca ataaatgcac
    15721 ggaaacgctg ggcatccccc gtttgtaata cgtaattaaa cagggctttt agctgtctgt
    15781 cattagtgtt ccctgtaact agcagcgaaa caatttggtc gactaatccc aacagatcgc
    15841 gctgacgaat atgtttctga attaactcca acagcgccat tttgcgatgt tgcataatct
    15901 cgtcatccgg caccacggta atatctacca gcggaaaagc cgatgaataa attttgcggg
    15961 ctatagcagg ctcggcaaat tcatcaagcc agcaaagtga ataaggataa ggacttctgc
    16021 aaccatgata aaacagcatc gggatcacca atggaagctc tttatagcct gcatcaagat
    16081 ggttttgcat cgccgcaatg gaatagcgca tcatgcgaaa agccattaat tcttccggct
    16141 tactttggtg ctctatcact acataaatat aacctgctcc ctcctgcgtt ttcacagacc
    16201 acaagaggtc ggaataatat tgccgcaggt cttcatcaat aaaactgttt ggttccagtt
    16261 ttagcgttgt taaatcacac agtttgcgca gcggcgcggg aagatgaata tcaataaaat
    16321 cccgcgcggt gtctggatgg cgtaaaaaag atttaaatac cgcatcatgc ggcgtggaag
    16381 ttgtcgatat tgtcatggcg ttccgtcacc cctcaaattt aagatgacgc gacaataacc
    16441 acgattaata cccaatgcag caggcagttt ttatctttgg acagcgaatt cagaactatt
    16501 tttagcggac tgcaactttt acatttcagc ggaagctgac tcgcaaattc cccttgaaaa
    16561 tactccggca gcgttagcca ccggaggggg aattatttca gcgatccctt aaggaactgt
    16621 tgcagacgag ggctttgcgg gttaccaaat aactgttctg gcgcgccctc ttcttctatt
    16681 ttcccctgat ggaggaaaat gacgtgagta gaaacatggc gagcaaagcc catttcgtga
    16741 gtcactacca ccatggtttt cccctcttct gccagttgct gcataatacg caacacttcg
    16801 cctaccagtt caggatcgag cgccgaggta ggttcatcaa acagtaaaac ttccggttcc
    16861 atcgccagcg cccgcgcgat agaaacacgc tgttgctgac caccggacag gtgcaccggg
    16921 tatttcccct gcgcacgttc gtctatcccg acttttgcca gatatttcac cgcccgctcg
    16981 cgcgcttcct gcttgctcag gcccaacacc tgaatcggcg cttccatgac gttttccagc
    17041 accgtcatat ggctccagag attgaagtgc tggaatacca tcgtcaggcg tgtgcgcagt
    17101 aagcgcagct gatttttatc ggcgactttg agttgaccat ctttgtcgcg caccagattg
    17161 atcgtctggc cattgaccac gatcgatcct tcgctcggtt tttcgaggaa gttaatgcag
    17221 cgcagaaagg tacttttccc cgatcccgac gatccgatga tgctaattac atctccggca
    17281 ttcgcttgca gtgaaacccc tttcagcact tcgtgttcgc cgtagcgttt atgcaaatcg
    17341 ataacgttta atttattctc ggacatcgta ttctcagtgc gttgaagaag gtttcacatg
    17401 ctgcaaccag cgtttttccg ctctgcgaaa gaggctgatc aggacataag agatgattaa
    17461 atagagcacc gcggcaatgc cgaaggcggt aaaaggttga tatgtggcgg cgttaatatc
    17521 gcgggcaatt ttcagcagat ccggcaccgt ggcagtaaat gccagcgcag tagagtgcag
    17581 catcaggatc acttcgttgc tgtacgccgg taacgcaata cgcagcgcag aaggcaaaat
    17641 aatgcagcga tacattttaa aggtcgagaa gccataggct ctggcggctt caatttcccc
    17701 atgcggcact gaacggattg ccccggcaaa aatctcggta gtgtaagcgc aggtgttaag
    17761 cgtcagcgcc agcacggtac agttcagacc actgcgaaaa aaggcgttaa ggaactcggt
    17821 tcccttaaca atctccagcg tgtacatgcc ggaatagaac accagcaact gaacatacag
    17881 cggcgtaccg cgaaaaatat aggtaaataa ccagattgga aactggatgt atttattact
    17941 tgagacgcga ccaatcgcca gaaacagcgc cagaactccg cctatcacta ccgacaaaat
    18001 aagcagccac agggtgatcg ccacgccagt aaagcgataa ccgtcggtcc acagcagtgg
    18061 tttccagtat tcatgtaaga tttcgatcac aggtcagccc tcttcacacc cacggagtag
    18121 cggcgctcaa ggaacagcag cacaccattg gaaacagtgg tgaaaaccag gtaaatcacg
    18181 ccacagacga tggcgaaata gaacggttcc caggtacttt tgcctgccag ctgagtggct
    18241 ttgaccacat cttccaggcc gagtaacgaa acgagtgcgg tagatttgag gatcacctgc
    18301 cagttgttgc caatgcctgg cagggcgtaa cgcatcatcg ccggaaacat gatccgtcga
    18361 aacacttgcc cacgagtaaa accgaacgcc gtcgccgcct ctatgtgtcc tttcggcact
    18421 gccataaacg cgccacgaaa cgtttcggta aaataagcac cgtagataaa accgagagtg
    18481 ataatacctg cgaccatcgg atcaatatca atctgcccga cgcccatcgc ctccgtcacc
    18541 gtgtttagcg caatctgcaa accgtagaaa atcagcagca tcaacaccaa gtccggcacg
    18601 cctcgaatca gcgtggtgta cccttcgaaa atcagccccg aaagccgatt ttgcgagagc
    18661 ttaccgccag cgccaattaa accgataatt acagcgagca ctacagagct gatagccagc
    18721 tccagcgtga cgagcgcacc ctgtaaaata acacctgaaa acccatacaa catgctgcct
    18781 gtcctgtcgt gtgtggtgaa ttaccgcctc ttgtctccct ccggttaccc gaagggagcg
    18841 gactttcttt caccgatggc gattaaccac cataaacatc aaaatcgaag tactttttcg
    18901 ctaatttctc gtaagtaccg tcagcgcgca tttcggcaaa ggctttgttc agtgcctcgc
    18961 gcagttcgtt atcttcttta cgcaggccca tgccggtccc tacgccaaac agtttttcat
    19021 ctttaacaga cggaccaccg aatttgtaat ctttaccgac gggttgtttg aggaaacctt
    19081 cgctggcagc gacctcatcc tggaacgcgg catcaatacg tccggcagtc aggtcagaat
    19141 aaatgttgtc ctgcccctga taagaaacga tttcaatgcc ttttggtgcc caatgttcgt
    19201 taccgaacgt ctcctgggtg gtgccctgca atacgcctac ccgtttgcct ttcagcgact
    19261 cgactgtcgg ctgaatgtcg gaatttttcg ccaccaccag acgagaatcg gcagcgtaca
    19321 gtttgtcggt gaaggcaatt tcttgctgac gtttttccgt aatggaaagc gatgacatga
    19381 tggcgtcaat cttcttcgct tttaaggacg ggattaacgc atccagtggg ttttcgacaa
    19441 acgtacattg cgtattgatg cgtttgcaca actccttcgc cagatcgata tcgaagccaa
    19501 ccagttcgcc ttgtgaattt tttgattcaa atggcgcata ggtcggatcg gtaccgatgc
    19561 ggatgttttg cggaatcgca gcaaacgccg cagttgcgct ggagaaggcc agaaccagag
    19621 agagcgatag caccagtttt ttcataatgt cctcaactga cagtcttttt atagggaata
    19681 tttacaggtt tgttacactt atcgtgccat aaaattgacc atcaaggcaa aatatcccgc
    19741 cccgttagac gtattatttt tcattaaaaa tgcttaagta tgcatttcaa tgcaccacga
    19801 tagtgcaaca aacctggcgt gcaccatgat gatcattcca tcaggtacag tttccctgcg
    19861 acgtatcagt caccgtagac attaaagtcg aaatactttt tcgccatctt gtcgtaggta
    19921 ccgtcctgac gcagctcgcc aagcgcctta ttaaacgccg ccgtcagttc agcatcatct
    19981 ttacgtagcc ctacaccggt accgtcaccg aagtattttt tgtctttcac tgatgagcca
    20041 gcaaaggcga aatctttacc agcaggttgc ttgaggaaac cttcgctggc cgccacttca
    20101 tcctgcagtg cggcatccag acgtcctgca gccagatcgg aatagaccaa atcctggttg
    20161 gcataggcca ccacatccac acctttgcta cgccaggtct cgttagcgta agcttcctgg
    20221 gttgatccct gcagcacacc cacatgttta cctttcagtg aatccagcgt tggctgaatc
    20281 ggcgaacctt tggcggcaat caaacgagaa tctgcggcgt acaacttgtc ggagaaagca
    20341 atctcctgct gacgtttatc ggtaatggag agcgacgaaa taatggcgtc gatttttttc
    20401 gctttcagtg aggggatcag cgcgtcaaag tcactggcaa cccaggtaca tttcacctgc
    20461 atccgtttgc acatctcgtt accgagatcg atatcaaagc caacaaagtc acctttggca
    20521 tctttcgatg agaacggcgc ataggtggta tcggttccga tacgtaccgt ctccggtagc
    20581 gccgcatagc tggaagccac tgcggagaga ccgactaaca aagacagagc gagaatcgac
    20641 ttcttcatac ataaccctca agtgaaaatg gcttttttat gttgtgtatt gtgttgtgtg
    20701 tttgcaggct ctttcatgca ggtcttatgc catctttacg acaacagtaa cattcaacgt
    20761 taaatatgtt aataagacgt tgcattattg tcctaaagtt gaagatagca ggtatggcgg
    20821 ttgaatcgca gcgtttcgat tcatcgaaag gaacaaaatg tcgaggattt gatcgcggtt
    20881 gcaaaattgc cctgaaacag ggcaacaatg aggttatgcg ccctgccagc gggcaaagag
    20941 atcttcagga agggttatcg caaactggtc aagaacacga ttaaccgtct gatttatcac
    21001 atcatcaagg gattgtgggc gatgataaaa cgccggaacg ggaggcataa tcaccgcacc
    21061 gatttctgcc gcctgagtca ttaaacgcag atggcctaag tgcagtggcg tttcacgcac
    21121 gcagagcacc aacgggcgac gctctttcag caccacatct gccgcacggg tcagtaagcc
    21181 atcagtatag ctatggacaa tgccggacag ggttttgatt gaacagggta aaatcaccat
    21241 ccccaacgtc tggaaagaac cggaagaaat gctggcagca atatcgcgcg catcgtgtgt
    21301 cacatcagct aacgcctgca cttcgcgcag ggaaaaatcc gtttcgaggg ataaggtctg
    21361 gcgcgctgcc tggctcatta ccagatgcgt ttcgatatct gtgacatcgc gcagaacctg
    21421 taataaacgc acgccataaa tcgcgccgct ggcaccgctg atgcctacaa tgagtcgttt
    21481 cataaatagt tgccctgtca gacttgcagg cagactttgc aggatttcga cgggagttgc
    21541 aagtcagggt gccagaccgg caccctgagc gaaggcatca tccttcgtta tgcatttcga
    21601 gattttccac ttcgttctga cgttgcactg ctttggcgtc gtcattacgt aaagtatcga
    21661 ggaaatcgag gtagccctga tcaacgtctt tggtgacgta gacgccgttg aataccgagc
    21721 attcaaactg ctggatatcc gggttttcag cgcgaacggc ttcgatcaga tcgttcagat
    21781 cctggaaaat caacccgtca gcaccgatga tctggcgaat ttcatctact tcgcgaccgt
    21841 gagcgatcag ttccgtggcg ctcggcatat cgataccgta aacgttcggg aagcgaattt
    21901 ccggtgccgc agaagcgagg tacactttct tcgctccggc ttcgcgtgcc atctcgataa
    21961 tctgctcaga agtggtgcca cggacaatgg agtcgtcgac cagcaagacg tttttatcgc
    22021 ggaactcggc gcggttggcg ttcagtttac ggcgcacgga cttacgacgc agctgctggc
    22081 ccggcatgat gaaggtgcgg ccaacatagc ggtttttaac gaagccctgg cggtacggtt
    22141 tgcccagaat acgagcaatt tccagcgcga tatcacacga ggtttccgga atagggatca
    22201 ccacgtcgat atccagatct tcccattcgc gggcaatttt ctcgcccagt ttggtgccca
    22261 tattcacgcg cgcgctgtaa acggaaattt tgtcgatgaa cgagtccggg cgggcaaagt
    22321 atacatactc aaacaggcac ggattgctga ccggattgtc agcacattga cgggtaaaca
    22381 actgcccttc ttcagtgatg taaatcgctt cgcccggcgc gacgtcacgc aggaaatcaa
    22441 agcccagcgt atcgagcgca acgctttcgg aagcgaccat atattctgta cggttctcgt
    22501 caatatcacg ttttcccagt accaacggac gaataccgtt tggatcgcgg aaagcaacca
    22561 taccgtggcc gatgatcatc gccacacagg catacgcgcc gcggattaag cggtttgtag
    22621 cagcgatggc ggcgaaaatg ttgtcggctt ccagcggata gtggcggaag ttgtccagtt
    22681 cgctggcaaa gatattaagc agaatttccg agtcggaagt ggtgttgatg tggcggcgtt
    22741 tttcttcaaa cagtttttta cgcaactcgt gagcgttggt cagattgccg ttgtgggcaa
    22801 gcgtaatgcc atacggggag ttaacgtaaa acggctgcgc ttcagaggcg ctggagctgc
    22861 cagccgtagg gtaacgcaca tgaccaatgc ccatattgcc ctgcaaacgc tgcatatggc
    22921 gagcttcaaa tacatcgctc accagcccgt tcgctttacg caaacggaag cagttattgg
    22981 catctatggt gatgatgccg gcggcatcct gaccgcgatg ctgaagcacc gttaaggcat
    23041 cataaatcga ctggttaacc ggcataacac cggcgatacc gacaataccg cacatacgtc
    23101 tttttcctcg ttaagccaca tctcagagca cttacgctct gggcaagaaa cttgacgagc
    23161 tttgcagata atcaaaaaac catctgatga taaaactgaa ctgcgggatc agctgtgatt
    23221 tgctccagtc ttcgcttttc gacaccccgg taaaggagtc gagaaagaag agaatggcgg
    23281 caacaatcaa cacaccgcgc aacgcaccga aacagacgcc cagcacccga tcggtgcctg
    23341 acaaccccgt tttctctacc aactggccta tcacgaagtt cacgatagca ccaacgatca
    23401 gggtagcgat aaacagtacc gcgatggcaa tcccatttcg aaccagttcg tcttcaaagc
    23461 ccgtaaacca gactgacagg taagtgtagt aatgactggc aacaaagaaa gcacaacccc
    23521 atgtcaccag cgataacgct tcacgaacaa agccgcggat caggctaacc agagaggaaa
    23581 aagcaatcac cgcgattatg gcgtaatcaa tccagaccat atgtgtccca cgattttacg
    23641 ccctgtcatc ctgttcgggg cgcattctaa cagaaaaaga aaacgtttgc gtagggattt
    23701 ccttcccgcg catcaataaa aatggcgctg aaaaaacatt caacgccatc gactttttat
    23761 gcctttgcgg catcgggcaa tgcgtgtcgg atgcggcgtg aacaccttat ccgacctacg
    23821 gttctacccc tgtgtaggcc tgataagacg cgccagcgtc gcatcaggca agaccgtatt
    23881 aattcggcgt atagcccatt accacgccac ttaagccaga aagttgcttc aactcaccca
    23941 gcgatccttt cagcttatct ttcgaggcat caggcccaac cagaatacgg gtaattttac
    24001 cctgcactgg cgtggatggc gacgtataaa cccgataacc ggcaccgcgc agcttaccga
    24061 caatctcatt cactttatcg gcatttttca gcgcgcccag ttgcacaaca taggctttac
    24121 ccgttggcgc tgctttttct tccacgaccg gcttcgacgc aggtggtgct tcaaccttcg
    24181 gtttaggcga ctccaccggt ttcggcttcg gtggggcgac cggtgcaggt tccggttcaa
    24241 actcggtgtt attgactgca atagtggccg gatcgagcga cggtgctgcg gcatcacctg
    24301 cccgcacctc ttccgctgcg ccttccggcg gctgcgtcgg cagcgcctgg gtggcggctg
    24361 gcatcatatc aggctcatcg cgatcgcccg ctttcggcac cagcgggata gccgcgaact
    24421 catcctgata atgttttttc tgcccgtcca gcagccctgg aagtacaatc acccccagcg
    24481 ccaccagcac gatcgtgccc actaaccgat tctgaaactt acttgccacc gcttctcctc
    24541 gcgtcaatca cttccatgac atgtgcgacc gtgtggaacg aaccacacac cagcacggta
    24601 tcttccgctt tagcgtccgc cattgcggca tcccatgcct gcgccacgct atcaaatgat
    24661 ttgccgttac ccaaatgctc aagcagttgt tctgccgtgg caccgcgcgg cccttccagt
    24721 ggcgcacaat accagtcatc aaccacgctt ttcaaccagg ccagagttcc ggcaatatct
    24781 ttatcatgta gcataccgat aaccgccagc acgcgcccgt ttttcggtag cgctttcata
    24841 cgcccggtga gatattccgc cgcatgtgga ttatgcgcga catcaaaaat aacgcgtggc
    24901 gactcgctca caatctggaa acgtcccggc aaaattgcgc tggcaatccc gtcgcgaatg
    24961 gcattttcac tgacctccag cccgctggca cgcagtgccg ccagcgccgt tgcggcattc
    25021 ggttgcggga caagcggcaa cggcagattt tccagcgtgc cgtgagcatc gctaaacgcc
    25081 cagtcatgat cggtgacgga atagttccac tcaacgcccc gacgttgtaa cagtgcactt
    25141 ttttcctgcg ccacatcagc aatggtagaa ggcatttccg gctcaccgac aattgccggt
    25201 ttttcgctgc ggaagatgcc tgctttctcg cgaccaatgc tttcgcgatc tggccccagc
    25261 cagtcggtat gatccagcgc aatgctggtt actaccgcaa catcggcatc gacaatattg
    25321 gttgcgtcca gacgaccgcc cagccctact tccagaatca ccacgtcaag ttgtgcctgt
    25381 ttgaacagcc acaacgccga cagcgtaccg tactcgaaat aggtcaggga aatatcaccg
    25441 cgtgccgatt caatctccgc aaaagaggcg gtgtgggccg attccggcaa ttcctggccc
    25501 tgcacacgta cgcgctcggt ataacgcacc agatgcggcg aagtgtagac gcccactttg
    25561 taccctgccg ccatcagaat cgactccagc gtacggcaag tggtgccttt gccattcgta
    25621 cccgcaacgg taaacacaaa tggcgcaggt ttcaggacgc caagacgcgc cgcgacctgg
    25681 ctcacgcgct caaggccgag atcgatagtt ttactgtgca ggttttccag ataagaaagc
    25741 cacgaagcca gaggcgacgc ggcttgagga gtgcgtttga taatcatggt atccgctgat
    25801 tcgttacggt gagaatagca aaagggcaga gccagtggcc ctgcccttat cagttatcag
    25861 gcctcaggtt cctgatctgg taccgatggc accactacgc cttcacgcgg cgcttcagga
    25921 ttcggcgctg gcagattcat caacttcgcc agaatgctcg ccagtttcag gcgcatttcc
    25981 ggacgacgga cgatcatgtc gatcgcgcct ttctcgatca ggaattcact gcgctggaat
    26041 cctggcggca gtttttcgcg aacggtctgt tcgataacac gcggaccggc aaagccgatt
    26101 aacgctttcg gttcagcgat gttgagatcg cccagcatgg cgaaacttgc agaaacaccg
    26161 cccatggtcg ggtcggtcag cacggagatg tacggcaagc cgcgctcctg catttttgcc
    26221 agcgccgcag aggttttcgc catctgcatc agcgacatca gtgcttcctg catacgtgcg
    26281 ccaccagagg cggagaagca gatcagtggg cagttatctt ccagcgcctg ctcaacggca
    26341 cgcacgaaac gtgcacctac cacagacccc atggaaccgc ccataaaggc gaactcgaat
    26401 gccgcagcga caactggcat tccatacagg gtgcctttca tcaccaccag cgcatctttt
    26461 tcgccggttt ctttctgcgc agatgccaga cggtctttat acttcttgga gtcacgaaac
    26521 ttcagcacat ctttcggctc aagctcgcta cccagctcca caaggcttcc ttcatctaac
    26581 aggctatgca ggcgattacg cgctgtcata cgcatgtgat ggtcacactt cggacagacc
    26641 tcaagattac gttccagctc agcgcggtat aaaacctgac cgcagctatc acacttagtc
    26701 cacacccctt caggaatgct cgccttgcgg gtgggagtaa tgttgctttt aattcgttca
    26761 atccagctca ttagggacct ttctgtctga acctggttcg atgccagttt tatctttggg
    26821 gacgcataat gccatttttg cccccaacag accatgaatg ttgcacatta aaacataaca
    26881 gcccgaaact ttggataaaa aagtggtcga accgcggagt tactttttat tttgcggcgc
    26941 gtgccgcagc gcgtttgtgg cgaataaatt cgaaaacacc tggcaatatt gaaacaaaaa
    27001 taatgccaac aatcatcagt ttaaggttgc tctgaatgaa ggggagcgtt ccgaagaaat
    27061 aacccgcgta ggtaaaaatc agtacccata gcaacgcgcc gatgacgtta aaagcggcga
    27121 aatgacgata cgacatgtgg cccattcccg caacgaacgg cgcgaacgtt ctgacgatcg
    27181 gcacaaaacg ggcgagaata atcgttttgc cgccatgttt ctcataaaac tgatgagtct
    27241 tatcaagata actgcgacga aagattttcg aatccggatt actgaacagt ttgtcaccaa
    27301 ataaccgtcc aatggtgtag ttcaccgcgt cacccacaat cgcggcgatc agcatcagca
    27361 ccaccatcat atggacgttg agatcgttgg tttccagtga tgccaacgca ccggcaacaa
    27421 acagcaatga atcacccggt aaaaacggcg ttaccaccag accggtttca cagaacaaaa
    27481 ttaagaacaa aatggcataa acccagacgc catactccgc gaccagttct gccagatgca
    27541 cgtcaatgtg caggataaaa tcgatgagga aataaatcag gtccatatta gcctatgcct
    27601 tgtactcgtc atctttcagg ctgtaactac gttggctaca gcctgaaatg ttccgagcat
    27661 tattcttgtt agtccgccag aaatagcggg cccattggcg gttttggaag atcataccgg
    27721 tcagggtaat ccaccgcgac cagatacagc ccttccgctt ttgccgttgc tgccgccagc
    27781 gttctgtcct ttgccgccag caactctgct atccagctct ccggctggtt gtgggcaccg
    27841 acttccatca ggctgccgac aatattcctg accatatgat gtacaaaggc attcgctttg
    27901 atatctacca ccacataagg accgtgacgc gtgacattaa tatgcataac gttgcgccac
    27961 ggggttcggg actggcactg caccgcacgg aacgaggtga aatcattctc gcccagcaag
    28021 cattgcgcag cccgatgcat ccgttcagcg tccagcggtt cgtaaaaatg ggttacccct
    28081 ttactcagta ccgccgggcg cagccgatga ttgtagatga tgtagcgata acggcgagcc
    28141 gtggcgctaa atcgggcatg aaaatcatca ggtacagctt taacccaacg cacggcgatg
    28201 tcaccaggta aattcgcatt tacgcccaac gtccacgcag cgtctttgcg ctgtgcggtc
    28261 gtttcgaaat gcacaacctg cccggtaccg tgtacaccgg cgtcagtacg cccggcgcag
    28321 aagacggtga tgggttcgtt cgccacctgg gagagcgcct tttccagctt ctcctgcaca
    28381 ctgcggactt cattctgccg ttgccagccg taatacctac tgccgtcgta ctcaatgccc
    28441 agcgcaattt tataaactgg cggttgttgc tggtcggaca ttagtacaga tactcctgca
    28501 ccagtttctc ggcgattttt actgccatca gcgcgccgcc aaagcgaacg ttatcggcca
    28561 ccgaccagaa ctgaacttgc tccggcatac cgtagtcatt acgtacgcag ccaacagaaa
    28621 gatgcggcgt accagaagca tctcctacct gagtcgggaa ttcgttctct tcagagagca
    28681 caatatcttc gccctgagca aacgcatcac gcgcttcttc tgccgccagc ggacgcagtg
    28741 cttcaaagtt gaccatctgg gcatgaccgt agaataccgg tgcctggacg acgcttgccg
    28801 aaatcatcag cccttcgtcc tgcaggattt tgcgtacttc gtcaacgata cgacgttctt
    28861 cacgcacgct accttcgcta tccggcagta acggcagcat gttgaacgcc agctgacgcc
    28921 cgaagaaatc ttcttcgtca atcggaatgc cattgagtaa tttagcactc tgccccgcta
    28981 acgcatcgac cgcttttttg ccctgggcgg aggctgaaat caggctggta acgctgatac
    29041 gtgataaacc gccctgatca attagcggtt tcagtgccgc cagcagctgg ctggtcagac
    29101 tgtccggtac ggcgatgaca ttccggttgc ggtaatcggt cagtacaaac gggtttactt
    29161 ccggcactac cagcggtacg tcaggttcga gagcaaacaa tccgctgctg tcgatcacca
    29221 gacaacctga gttggtcgct tcttcaaccc aggcagcggt agcttctttg cctgcgacaa
    29281 aaaatgccag ctgcgcctgc gtccagtcaa attcagcggc atcctgcacg gtgattgtct
    29341 taccaccaaa gcgcagttgt tcgcctgcgc tttcgttacg tgccagtgca taaatttccc
    29401 caaccgggaa ctgacgttca gccagcgttt caagcagggc ttcgcccaca gcgccagttg
    29461 cgcccaggac ggcaatgttc cagccttcag acatggtggt ttactccaga aatagcaaag
    29521 ctccctgcca atgttacagc agagagcatg aagaagagat taacgtgccg gatgatgaac
    29581 ggcgttaaaa cccagtttac acagcaatga tgccgcactg gcgtcatcac aaattacata
    29641 cagagatgac cattcacggc gctcaagata gtttttgcgc agtttatcga actcacccgg
    29701 tatcccggcg actttacgca gtggcgcatc atcgcggcgc acatcataca ccaaatgcac
    29761 cagccttttc agcgtcggtt gatcgagcgg accatgcagc gtaatgcgac caaactctgg
    29821 cgcgggcagt aatgtatcca gcgcaacgtg ttgttcatgc ccaatgaatt tgctataggc
    29881 ttcaaacact tgcgtagtac cgcgtgcttt accttccagg gtatagcctg caatatgcgg
    29941 cgtgccgata tccacttttg tcagcaattc cacattaagt tccggctcgc cttcccagac
    30001 atccagcacc acgcttaact tctggccttc attcaggcag gtcagcagcg cggtattatc
    30061 gacgactgcg ccacggcagg cgttaatcag aatcgctccg ggcttcaggc tacggatcag
    30121 tttttcatcc gccagatgtc gcgttttgta cggaccatct ttaaagagtg gcgtatggaa
    30181 agtcagaata tccgcatgct ggactaattc atccagcgag cggaaatcgc cctcatcccc
    30241 acggtcggcg cgaggcggat cgcaaagcaa ggttgtaatc cctaacgctt ccagtcgtgc
    30301 ctgtaaacgc cgaccaacgt taccaacgcc cacgatcccc acggtacgct cgtgcagtga
    30361 aaatccatcg cgttcagcaa gcatcagcag tgaggagaaa acatattcca ctactgcaat
    30421 cgcattacag ccaggtgcag cggaaaaacc aattcccgcc tgctttaacc atgtgtcatc
    30481 aacatggtct gtccccgcgg tggcagtgcc aacaaattta atgggttttc ctgccagcaa
    30541 agattcattc actttcgtga ccgaacgcac catcagcgca tccgcgtctg ccagttgagc
    30601 cacggggatg ggacgcccgg gaaccgcggt cacctcaccc aaacggctaa ataagtcgcg
    30661 ggcataaggc atattttcat caacaaggat cgttaataat ttttcagtac gcgacaatct
    30721 ggccccccct ttatcagcaa acaccctttt aaccccaccg tacaacatga aaaccatttc
    30781 ataatcatac acatacaaac gcaaccattt agacgggtga tactggaaag tatgtttcac
    30841 agtatcaccc ttaaagctat ttcagaccag acaaaatcac tctcccgtgg acaccgagtg
    30901 gacacttacg ccacctttca gcggattaag agccaccgca tcctgcaaat aatccggcgc
    30961 aaaatgtgca tacgccattg tttgctggat agtcgcgtga cccaggatcc tctgtagcgc
    31021 aataatgttt cccccgttca tcacaaaatg actggcgaac gtatgtcgca acacatgcgc
    31081 cgcctgtcct tttggcaaat cgggtttaac ccttttcagc accaggcaaa attcccggta
    31141 cttcacatca aataatcccc ccgtctcgct ggttttgatc gcctcacaaa ccgcctgcga
    31201 gattggcacc gttctcttac gaccgttttt ggtctcaaga aacgtcacac gattatgaac
    31261 tatctgttca ccacgaagct tactggcttc gccccagcgt gcccccgtac ttaaacacaa
    31321 aagcgcaaca cgccagtaat cgccctctag tgtatcgagc aataacgcca cttctttctg
    31381 ggacaagaaa gccatttctc gcggggacac ataaagcaca gagattcccc tgaccgggtg
    31441 ctctgcatcc catagtccta tttttttcag agcggtaaac attccggata accggttcat
    31501 atacctgtta gccgatgacg gcttcagtcc gtcagcgatc ctgcgagaac gccaggcaat
    31561 gattttcagc ttatccagat ccaccgcctg catatcagca ccaagttcat tgattatgtt
    31621 gcgtaattgt tttcggtctt cctccgcttt acgcctgtgc tggccgtgat acacccacca
    31681 caactccagc aaatcgttca acgtccggcg atcacggtag ccctgtatat attcccgctt
    31741 ctcagcattc gccatgatgt agcgctcagt ggccaccgct accgattttt tgttaaatac
    31801 cttacgcacg cgttttccct tgcgtccgtt cggcctgata tccagcaaat aacgaccatc
    31861 ttcgagcttc ttaatcgaca tggcgaagcc ctccaatgaa ccgttttaca atttcaccag
    31921 cttcttccca gcaatagtca gaccaggcaa aaagcaggtc caaccagttt tctggcctca
    31981 gcggggtaat tttcgggttg tttcggttga gtcctgattg ccctccaaat tgcaggagcc
    32041 atcaagagag agagccgggg ctatctgtcc ggcctccggc attgtttcat cagttgataa
    32101 ccagtaggcg tacttcttaa atttagggtg ttttgtaacc ttaagtaagg ccccctctgt
    32161 tacttgcttc cccctcacct catagttagt tactgtccca taaggcaacc ccacgcaatc
    32221 cgcaaactcc tggcgggtca taccttcagc ttcacgtatc aggcgaaatt tttcacccat
    32281 gcttgacaaa gatgccatat cgggcatatc ctccattgca aacgtgccac atcgggcaca
    32341 cacataagaa gccaaaataa gccactttgg gccattttga gccattcgaa cgaatcaagg
    32401 agattaccac aatgagagaa tcagagcttg agggcttcat tcaggtagcg ccatatccac
    32461 ttgaggcggt gccatatcaa ctattcgcca agatgattgg ccgcaaagaa tccaccgtca
    32521 gaaccatgat tgacgccgca aagctaccaa caattgactt tgtgaaaccg ggttcagtga
    32581 aaactcgtgc atcagaaaac tgggtatatc tgccagcatt taacgcaggt atgcgaaaag
    32641 cattttttaa tcaacccaaa gaacaacgcg atgcatggct gttatggctg ggtctgtaac
    32701 gatatatcca ggaatttttc aaaaccaata tatctactct gagagagttg tttgggcata
    32761 aaaaagcaca ggataactaa gctgataacc cactacagac acaaaaggaa acagccatga
    32821 ctacacaaaa agcaaaaacc ataattaaac gatacaacgt acgtagtggt gtggctggcg
    32881 ttgtcttttc cgatgtgaca cagcgacgca gcttccgcac tggcacagaa cgccacgcag
    32941 cccgtttctc aaccagtgca tcacgcagca acacgcacta cagcctgcag gaaacgcacg
    33001 ccacgcctga cgggcatctg gttaaacaaa ccggtaaaca cacctggctg attaatgatg
    33061 ccggaattgt catacacaga ggcgcacgca acccgattac cggaaatacc tgttactccc
    33121 tgactcgcgg agatgagcag ttcgggcagg atttcaccct gcacgaagca ttaagaacgg
    33181 cagaccggtt aatcagcggt cgcagttttc agcacccgga acatcgccac tgataaataa
    33241 aaggattcag ccatgatcga agcctacaaa atacttatta ataatgcgtt gcagcgttat
    33301 cacttcgaac tcgcaaaccg gggtgtaacc cgggacgaag gacttcgtaa ctcactgcgg
    33361 ggcgcaatta tggggctgta taactgcgca ctgggcagcg atgaccgcga agcactggaa
    33421 gaattaaaag ttattgtggc cgaactgttt gaaggtgaaa tcgttgagcc ttatttcaca
    33481 ga
//
LOCUS       DAZFLD010000022        33350 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.022, whole genome shotgun sequence.
ACCESSION   DAZFLD010000022 DAZFLD010000000
VERSION     DAZFLD010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33350
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.022"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(30..2078)
                     /gene="prc"
                     /locus_tag="ACHKBL_000948"
     CDS             complement(30..2078)
                     /gene="prc"
                     /locus_tag="ACHKBL_000948"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HFO5817861.1"
                     /translation="MNMFFRLTALAGLLAIAGQTFAVEDITRADQIPVLKEETQHATV
                     SERVTSRFTRSHYRQFDLDQTFSAKIFDRYLNLLDYSHNVLLASDVEQFAKKKTELGD
                     ELRSGKLDVFYDLYNLAQKRRFERYQYALSVLEKPMDFTGNDTYNLDRSKAPWPKNEA
                     ELNALWDSKVKFDELSLKLAGKTDKEIRETLTRRYKFAIRRLAQTNSEDVFSLAMTAF
                     AREIDPHTNYLSPRNTEQFNTEMSLSLEGIGAVLQMDDDYTVINSMVAGGPAAKSKAI
                     SVGDKIVGVGQTGKPMVDVIGWRLDDVVALIKGPKGSKVRLEILPAGKGTKTRTVTLT
                     RERIRLEDRAVKMSVKTVGKEKVGVLDIPGFYVGLTDDVKVQLQKLEKQNVSSVIIDL
                     RSNGGGALTEAVSLSGLFIPSGPIVQVRDNNGKVREDSDTDGQVFYKGPLVVLVDRFS
                     ASASEIFAAAMQDYGRALVVGEPTFGKGTVQQYRSLNRIYDQMLRPEWPALGSVQYTI
                     QKFYRVNGGSTQRKGVTPDIIMPTGNEETETGEKFEDNALPWDSIDAATYVKSGDLTA
                     FGPELLKEHNARIAKDPEFQNIMKDIARFNAMKDKRNIVSLNYAVREKENNEDDATRL
                     ARLNERFKREGKPELKKLDDLPKDYQEPDPYLDETVNIALDLAKLEKARPAEQPAPVK
                     "
     gene            complement(2098..2796)
                     /gene="proQ"
                     /locus_tag="ACHKBL_000949"
     CDS             complement(2098..2796)
                     /gene="proQ"
                     /locus_tag="ACHKBL_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707288.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0033592 - RNA strand annealing activity
                     [Evidence IEA];
                     GO_function: GO:0034057 - RNA strand-exchange activity
                     [Evidence IEA];
                     GO_process: GO:0010608 - post-transcriptional regulation
                     of gene expression [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HFO5817862.1"
                     /translation="MENQPKLNSSKEVIAFLAERFPHCFSAEGEARPLKIGIFQDLVD
                     RVAGEMNLSKTQLRSALRLYTSSWRYLYGVKPGATRVDLDGNPCGELDEQHVEHARKQ
                     LEEAKARVQAQRAEQQAKKREAAAAAGEKEDAPRRERKPRPTTPRRKEGAERKPRAQK
                     PVEKAPKTVKAPREEQHTPVSDISALTVGQALKVKAGQNAMDATVLEITKDGVRVQLN
                     SGMSLIVRAEHLVF"
     gene            complement(2893..3390)
                     /gene="msrC"
                     /locus_tag="ACHKBL_000950"
     CDS             complement(2893..3390)
                     /gene="msrC"
                     /locus_tag="ACHKBL_000950"
                     /EC_number="1.8.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine (R)-S-oxide reductase"
                     /protein_id="HFO5817863.1"
                     /translation="MNKIEFYADLNRDFNALMAGETSFLATLANTSALLYERLTDVNW
                     AGFYLLEDDTLVLGPFQGKIACVRIPVGRGVCGTAVARNQVQRIEDVHVFDGHIACDA
                     ASNSEIVLPLVVKNQIIGVLDIDSTVFGRFTDEDEQGLRQLVAQLEKVLATTDYKKFF
                     ASVAG"
     gene            3520..4803
                     /gene="yebS"
                     /locus_tag="ACHKBL_000951"
     CDS             3520..4803
                     /gene="yebS"
                     /locus_tag="ACHKBL_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity lipid transport subunit YebS"
                     /protein_id="HFO5817864.1"
                     /translation="MALNTPQITPTKKITVRAIGEELPRGDYQRCPQCDMLFSLPEIN
                     SHQSAYCPRCQAKIRDGRDWSLTRLAAMAFTMLLLMPFAWGEPLLHIWLLGIRIDANV
                     MQGIWQMTKQGDAITGSMVFFCVIGAPLILVTSIAYLWFGNRLGMNLRPVLLMLERLK
                     EWVMLDIYLVGIGVASIKVQDYAHIQAGVGLFSFVALVILTTVTLSHLNVEELWERFY
                     PQRPTTRRDEKLRVCLGCHFTGYPDQRGRCPRCHIPLRLRRRHSLQKCWAALLASIVL
                     LLPANLLPISIIYLNGGRQEDTILSGIMSLASSNIAVAGIVFIASILVPFTKVIVMFT
                     LLLSIHFKCQQGLRTRILLLRMVTWIGRWSMLDLFVISLTMSLINRDQILAFTMGPAA
                     FYFGAAVILTILAVEWLDSRLLWDAHESGNARFDD"
     gene            4772..7405
                     /gene="yebT"
                     /locus_tag="ACHKBL_000952"
     CDS             4772..7405
                     /gene="yebT"
                     /locus_tag="ACHKBL_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding membrane homeostasis protein YebT"
                     /protein_id="HFO5817865.1"
                     /translation="MSQETPASTTEAQIKNKRRISPFWLLPFIALMIAGWLIWDSYQD
                     RGNTVTIDFMSADGIVPGRTPVRYQGVEVGTVQDISLSDDLRKIEVKVSIKSDMKDAL
                     REETQFWLVTPKASLAGVSGLDALVGGNYIGMMPGKGKEQDHFVALDTQPKYRLDNGD
                     LMIHLQAPDLGSLNSGSLVYFRKIPVGKVYDYAINPNKQGVVIDVLIERRFTDLVKKG
                     SRFWNVSGVDANVSISGAKVKLESLAALVNGAIAFDSPEESKPAEAEDTFGLYEDLAH
                     SQRGVIIKLELPSGAGLTADSTPLMYQGLEVGQLTKLDLNPGGKVTGEMTVDPSVVTL
                     LRENTRIELRNPKLSLSDANLSALLTGKTFELVPGDGEPRKEFVVVPGEKALLQEPDV
                     LTLTLTAPESYGIDAGQPLILHGVQVGQVIDRKLTSKGVTFTVAIEPQHRELVKGDSK
                     FVVNSRVDVKVGLDGVEFLGASASEWINGGIRILPGDKGEMKASYPLYANLEKALENS
                     LSDLPTTTVSLSAETLPDVQAGSVVLYRKFEVGEVITVRPRANAFDIDLHIKPEYRNL
                     LTSNSVFWAEGGAKVQLNGSGLTVQASPLSRALKGAISFDNLSGASASQRKGDKRILY
                     ASETAARAVGGQITLHAFDAGKLAVGMPIRYLGIDIGQIQTLDLITARNEVQAKAVLY
                     PEYVQTFARGGTRFSVVTPQISAAGVEHLDTILQPYINVEPGRGNPRRDFELQEATIT
                     DSRYLDGLSIIVEAPEAGSLGIGTPVLFRGLEVGTVTGMTLGTLSDRVMIAMRISKRY
                     QHLVRNNSVFWLASGYSLDFGLTGGVVKTGTFNQFIRGGIAFATPPGTPLAPKAQEGK
                     HFLLQESEPKEWREWGTALPK"
     gene            7485..8924
                     /gene="rsmF"
                     /locus_tag="ACHKBL_000953"
     CDS             7485..8924
                     /gene="rsmF"
                     /locus_tag="ACHKBL_000953"
                     /EC_number="2.1.1.178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1407)-C(5))-methyltransferase
                     RsmF"
                     /protein_id="HFO5817866.1"
                     /translation="MAQHTVYFPDAFLTQMREAMPSTLSFDDFLAACQRPLRRSIRVN
                     TLKTSVADFLQLTAPYGWTLTPIPWCEEGFWIERDSEDALPLGSTAEHLSGLFYIQEA
                     SSMLPVAALFADGNAPQRVMDVAAAPGSKTTQIAARMNNEGAILANEFSASRVKVLHA
                     NISRCGISNVALTHFDGRVFGAAVPEMFDAILLDAPCSGEGVVRKDPDALKNWSPESN
                     QEIAATQRELIDSAFHALRPGGTLVYSTCTLNREENEAVCLWLKETYPDAVEFLPLGD
                     LFPGANKALTEEGFLHVFPQIYDCEGFFVARLRKTQAIPALPTPKYKVGNFPFSPVKD
                     REAGQIRQAAASVGLNWDENLRLWQRDKELWLFPVGIEALIGKVRFSRLGIKLAETHN
                     KGYRWQHEAVIALASPDNVNAFELTPQEAEEWYRGRDVYPQAAPVADDVLVTFQHQPI
                     GLAKRIGSRLKNSYPRELVRDGKLFTGNA"
     gene            9042..9278
                     /gene="yebV"
                     /locus_tag="ACHKBL_000954"
     CDS             9042..9278
                     /gene="yebV"
                     /locus_tag="ACHKBL_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebV family protein"
                     /protein_id="HFO5817867.1"
                     /translation="MKTSVRIGAFEIDDGELHGESPGDRTLTIPCKSDPDLCMQLDAW
                     DAETSIPALLNGEHSVLYRTRYDQQSDAWIMRLA"
     gene            9383..9574
                     /gene="yebW"
                     /locus_tag="ACHKBL_000955"
     CDS             9383..9574
                     /gene="yebW"
                     /locus_tag="ACHKBL_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebW family protein"
                     /protein_id="HFO5817868.1"
                     /translation="MFALVLFVCYLDGGCEDIVVDVYNTEQQCLYSMSDQRIRHGGCF
                     PIEDFIDGFWRPAQEYGDF"
     gene            complement(9575..10231)
                     /gene="pphA"
                     /locus_tag="ACHKBL_000956"
     CDS             complement(9575..10231)
                     /gene="pphA"
                     /locus_tag="ACHKBL_000956"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HFO5817869.1"
                     /translation="MKQPAPVYQRIAGHQWRHIWLSGDIHGCLEQLRRKLWHCRFDPW
                     RDLLISVGDVIDRGPQSLRCLQLLEQHWVRAVRGNHEQMAMDALASQQMSLWFMNGGD
                     WFIALADNQQKQAKTALEKCQHLPFILEVHSRTGKHVIAHADYPDDVYEWQKDVDLHQ
                     VLWSRSRLGERPKGQGIKGADHFWFGHTPLRHRVDIGNLHYIDTGAVFGGELTLVQLQ
                     "
     gene            complement(10626..10967)
                     /gene="yebY"
                     /locus_tag="ACHKBL_000957"
     CDS             complement(10626..10967)
                     /gene="yebY"
                     /locus_tag="ACHKBL_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebY family protein"
                     /protein_id="HFO5817870.1"
                     /translation="MMKKSILAFLLLTSSAAALAAPQVITVSRFEVGKDKWAFNREEV
                     MLTCRPGNALYVINPSTLVQYPLNDIAQKEVASGKTKAQPISVIQIDDPNNPGEKMSL
                     APFIERAEKLC"
     gene            complement(10980..11852)
                     /gene="copD"
                     /locus_tag="ACHKBL_000958"
     CDS             complement(10980..11852)
                     /gene="copD"
                     /locus_tag="ACHKBL_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
                     /protein_id="HFO5817871.1"
                     /translation="MLAFTWIALRFIHFTSLMLVFGFAMYGAWLAPLTIRRLLTKRFL
                     RLQQHAAVWSLISATAMLAVQGGLMGTGWSDVFSPNIWLAVLQTQFGGIWLWQIVLAL
                     VTLIVALMQPQNMPRLLFMLTTAQFILLAGVGHATLNEGVTAKIHQTNHAIHLICAAA
                     WFGGLLPVLWCMQLIKGRWRHQAIQALMRFSWCGHFAVIGVLASGVLNALLITGFPPT
                     LTTYWGQLLMLKAILVMIMVVIALANRYVLVPRMRQDEDRAAPWFVWMTKLEWAIGAV
                     VLVIISLLATLEPF"
     gene            complement(11856..12230)
                     /gene="yobA"
                     /locus_tag="ACHKBL_000959"
     CDS             complement(11856..12230)
                     /gene="yobA"
                     /locus_tag="ACHKBL_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopC domain-containing protein YobA"
                     /protein_id="HFO5817872.1"
                     /translation="MASTARSLRYALAILTTSLVTPSVWAHAHLTHQYPAANAQVTAA
                     PQAITLNFSEGVETGFSGAKITGPKNENIKTLPAKRNEQDQKQLIVPLADSLKPGTYT
                     VDWHVVSVDGHKTKGHYTFSVK"
     gene            12369..12599
                     /gene="holE"
                     /locus_tag="ACHKBL_000960"
     CDS             12369..12599
                     /gene="holE"
                     /locus_tag="ACHKBL_000960"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HFO5817873.1"
                     /translation="MLKNLAKLDQTEMDKVNVDLAAAGVAFKERYNMPVIAEAVEREQ
                     PEHLRSWFRERLIAHRLASVNLSRLPYEPKLK"
     gene            12701..13357
                     /gene="yobB"
                     /locus_tag="ACHKBL_000961"
     CDS             12701..13357
                     /gene="yobB"
                     /locus_tag="ACHKBL_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein YobB"
                     /protein_id="HFO5817874.1"
                     /translation="MSFWKVAAAQYEPRKTSLTEQVAHHLEFVSAAARQQCQLLVFPS
                     LSLLGCDYSRRALPAPPDLSLLDPLCYAATTWRMTIIAGLPVEYNDRFIRGIAVFAPW
                     RKTPGIYHQSHGACLGRRSRTITVVDEQPEGMDMDPTCSLFTTGQCLGEPDLLASTRR
                     LQFFSHQYSIAVLMANARGNSALWDEHGRLIVRADRGSLLLVGQRSSQGWQGDIIPLR
                     "
     gene            13381..14043
                     /gene="exoX"
                     /locus_tag="ACHKBL_000962"
     CDS             13381..14043
                     /gene="exoX"
                     /locus_tag="ACHKBL_000962"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease X"
                     /protein_id="HFO5817875.1"
                     /translation="MLRIIDTETCGLQGGIVEIASVDVIDGKIVNPMSHLVRPDRPIS
                     PQAMAIHRITEAMVADKPWIEDVIPHYYGSEWYVAHNASFDRRVLPEMPGEWICTMKL
                     ARRLWPGIKYSNMALYKTRKLNVQTPPGLHHHRALYDCYITAALLIDIMNTSGWTAEQ
                     MADITGRPSLMTTFTFGKYRGKAVSDVAERDPGYLRWLFNNLDSMSPELRLTLKHYLE
                     NT"
     gene            complement(14040..16100)
                     /gene="ptrB"
                     /locus_tag="ACHKBL_000963"
     CDS             complement(14040..16100)
                     /gene="ptrB"
                     /locus_tag="ACHKBL_000963"
                     /EC_number="3.4.21.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
                     /protein_id="HFO5817876.1"
                     /translation="MLPKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPKVLDYLQQEN
                     SYGHRVMASQQALQDRILKEIIDRIPQREVSAPYIKNGYRYRHIYEPGCEYAIYQRQS
                     AFSEEWDEWETLLDANKRAAHSEFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNL
                     ETGNWYPELLDNVEPSFVWANDSWTFYYVRKHPVTLLPYQVWRHAIGTPASQDKLIYE
                     EKDDTYYVSLHKTTSKHYVVIHLASATTSEVRLLDAEMADAEPFVFLPRRKDHEYSLD
                     HYQHRFYLRSNRHGKNFGLYRTRMRDEQQWEELIPPRDNIMLEGFTLFTDWLVVEERQ
                     RGLTSLRQINRKTREVIGIAFDDPAYVTWIAYNPEPETARLRYGYSSMTTPDTLFELD
                     MDTGERRVLKQTEVPGFDAANYRSEHLWIVARDGVEVPVSLVYHRKHFRKGHNPLLVY
                     GYGSYGASIDADFSFSRLSLLDRGFVYAIVHVRGGGELGQQWYEDGKFLKKKNTFNDY
                     LDACDALLKLGYGSPSLCYAMGGSAGGMLMGVAINQRPELFHGVIAQVPFVDVVTTML
                     DESIPLTTGEFEEWGNPQDPQYYEYMKSYSPYDNVTAQAYPHLLVTTGLHDSQVQYWE
                     PAKWVAKLRELKTDDHLLLLCTDMDSGHGGKSGRFKSYEGVAMEYAFLVALAQGTLPA
                     TPED"
     gene            complement(16309..16968)
                     /gene="yebE"
                     /locus_tag="ACHKBL_000964"
     CDS             complement(16309..16968)
                     /gene="yebE"
                     /locus_tag="ACHKBL_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HFO5817877.1"
                     /translation="MANWLNQLQSLLGQSRSSTSSSADQGLGKLLVPGALGGLAGLLV
                     ANKSARKLLTKYGTNALLVGGGAVAGTVLWNKYKDKIRAAHQDEPQFGAQSTPLDERT
                     ERLILALVFAAKSDGHIDAKERAAIDQQLREAGVEEKGRVLIEQAIEQPLDPQRLATG
                     VRNEEEALEIYFLSCAAIDIDHFMERSYLNALGDALKIPQDVREGIERDLEQQKRTLA
                     E"
     gene            complement(17295..17651)
                     /gene="yebF"
                     /locus_tag="ACHKBL_000965"
     CDS             complement(17295..17651)
                     /gene="yebF"
                     /locus_tag="ACHKBL_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YebF"
                     /protein_id="HFO5817878.1"
                     /translation="MKKRGAFLGLLLVSACASVFAANNETSKSVTFPKCEGLDAAGIA
                     ASVKRDYQQNRVARWADDQKIVGQPDPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHY
                     QVSVDCKAGMAEYQRR"
     gene            complement(17718..18008)
                     /gene="yebG"
                     /locus_tag="ACHKBL_000966"
     CDS             complement(17718..18008)
                     /gene="yebG"
                     /locus_tag="ACHKBL_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein YebG"
                     /protein_id="HFO5817879.1"
                     /translation="MAVEVKYVVIREGEEKMSFTSKKEADAYDKMLDTADLLDTWLTN
                     SPVQMEDEQREALSLWLAEQKDVLSTILKTGKLPSPQVVGAESEEEDASHAA"
     gene            18142..19320
                     /gene="purT"
                     /locus_tag="ACHKBL_000967"
     CDS             18142..19320
                     /gene="purT"
                     /locus_tag="ACHKBL_000967"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HFO5817880.1"
                     /translation="MTLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRY
                     ADAPAMHVAHRSHVINMLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEEGLNVV
                     PCARATKLTMNREGIRRLAAEELQLPTSTYRFADNENLFREAVAEIGYPCIVKPVMSS
                     SGKGQTFIRSAEQLAHAWEYAQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFC
                     APVGHRQEDGDYRESWQPQQMSQLALERAQEIARKVVLALGGYGLFGVELFVCGDEVI
                     FSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPVGGIRQYGPAASAVILPQLTSQN
                     VTFDNVQNAVGAGIQIRLFGKPEIDGSRRLGVALATAESVDEAIERAKHAAGQVKVQG
                     "
     gene            complement(19376..20017)
                     /gene="kdgA"
                     /locus_tag="ACHKBL_000968"
                     /gene_synonym="eda"
     CDS             complement(19376..20017)
                     /gene="kdgA"
                     /locus_tag="ACHKBL_000968"
                     /gene_synonym="eda"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFO5817881.1"
                     /translation="MKNWKTSAESILTTGPVVPVIVVKKLEHAVPMAKALVAGGVRVL
                     EVTLRTECAVDAIRAIAKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLK
                     AATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCP
                     TGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKLAREAVEGAKL"
     gene            complement(20054..21865)
                     /gene="edd"
                     /locus_tag="ACHKBL_000969"
     CDS             complement(20054..21865)
                     /gene="edd"
                     /locus_tag="ACHKBL_000969"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004456 - phosphogluconate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HFO5817882.1"
                     /translation="MNPQLLRVTNRIIERSRETRSAYLARIEQAKTSTVHRSQLACGN
                     LAHGFAACQPEDKASLKSMLRNNIAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVG
                     QVAGGVPAMCDGVTQGQDGMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPG
                     LTMAALSFGHLPAVFVPSGPMASGLPNKEKVRIRQLYAEGKVDRMALLESEAASYHAP
                     GTCTFYGTANTNQMVVEFMGMQLPGSSFVHPDSPLRDALTAAAARQVTRMTGNGNEWM
                     PIGKMIDEKVVVNGIVALLATGGSTNHTMHLVAMARAAGIQINWDDFSDLSDVVPLMA
                     RLYPNGPADINHFQAAGGVPVLVRELLKAGLLHEDVNTVAGFGLSRYTLEPWLNNGEL
                     DWREGAEKSLDSNVIASFEQPFSHHGGTKVLSGNLGRAVMKTSAVPVENQVIEAPAVV
                     FESQHDVMPAFEAGLLDRDCVVVVRHQGPKANGMPELHKLMPPLGVLLDRCFKIALVT
                     DGRLSGASGKVPSAIHVTPEAYDGGLLAKVRDGDIIRVNGQTGELTLLVDEAELAARE
                     PHIPDLSASRVGTGRELFSALREKLSGAEQGATCITF"
     gene            complement(22100..23575)
                     /gene="zwf"
                     /locus_tag="ACHKBL_000970"
     CDS             complement(22100..23575)
                     /gene="zwf"
                     /locus_tag="ACHKBL_000970"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HFO5817883.1"
                     /translation="MAVTQTAQACDLVIFGAKGDLARRKLLPSLYQLEKAGQLNPDTR
                     IIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFSRL
                     GAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEIN
                     DQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVG
                     IEGRWGYFDKAGQMRDMIQNHLLQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDR
                     SNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFY
                     LRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPG
                     LDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVD
                     SITEAWAMDNDAPKPYQAGTWGPVASVAMITRDGRSWNEFE"
     gene            23913..24782
                     /gene="hexR"
                     /locus_tag="ACHKBL_000971"
                     /gene_synonym="yebK"
     CDS             23913..24782
                     /gene="hexR"
                     /locus_tag="ACHKBL_000971"
                     /gene_synonym="yebK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HFO5817884.1"
                     /translation="MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAALALEA
                     NVSEPTVNRFCRSMDTRGFPDFKLHLAQSLANGTPYVNRNVNEDDSVESYTGKIFESA
                     MATLDHVRHSLDKSAINRAVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYS
                     DDIVLQRMSCMNCSDGDVVVLISHTGRTKNLVELAQLARENDAIVIALTSAGTPLARE
                     ATLAITLDVPEDTDIYMPMVSRLAQLTVIDVLATGFTLRRGAKFRDNLKRVKEALKES
                     RFDKQLLNLSDDR"
     gene            24910..26352
                     /gene="pyk"
                     /locus_tag="ACHKBL_000972"
     CDS             24910..26352
                     /gene="pyk"
                     /locus_tag="ACHKBL_000972"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HFO5817885.1"
                     /translation="MSRRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSP
                     EDHKMRADKVREIAAKLGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGK
                     GEGDKEKVGIDYKGLPADVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNK
                     GINKLGGGLSAEALTEKDKADIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDA
                     KIVAKVERAEAVCSQDAMDDIILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQL
                     NRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAM
                     ARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTA
                     LMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVHFDSANDGVAAASEAVNLLRDKGY
                     LMSGDLVIVTQGDVMSTVGSTNTTRILTVE"
     gene            complement(26484..27455)
                     /gene="lpxM"
                     /locus_tag="ACHKBL_000973"
                     /gene_synonym="msbB"
     CDS             complement(26484..27455)
                     /gene="lpxM"
                     /locus_tag="ACHKBL_000973"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416369.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HFO5817886.1"
                     /translation="METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMAGIALTPPKFR
                     DPILARLGRFAGRLGKSSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMAMMAE
                     LAIRGPEKIQPRVDWQGLEIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
                     FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSE
                     FVDFFATYKATLPAIGRLMKVCRARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHT
                     IARRMNEEVEIFVGPRPEQYTWILKLLKTRKPGEIQPYKRKDLYPIK"
     gene            complement(27575..28897)
                     /gene="mepM"
                     /locus_tag="ACHKBL_000974"
     CDS             complement(27575..28897)
                     /gene="mepM"
                     /locus_tag="ACHKBL_000974"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HFO5817887.1"
                     /translation="MQQIARSVALAFNNLPRPHRVMLGSLTVLTLAVAVWRPYVYHRD
                     ATPIVKTIELEQNEIRSLLPEASEPIDQAAQEDEAIPQDELDDKIAGEAGVHEYVVST
                     GDTLSSILNQYGIDMGDITQLAAADKELRNLKIGQQLSWTLTADGELQRLTWEVSRRE
                     TRTYDRTAANGFKMTSEMQQGEWVNNLLKGTVGGSFVASARNAGLTSAEVSAVIKAMQ
                     WQMDFRKLKKGDEFAVLMSREMLDGKREQSQLLGVRLRSEGKDYYAIRAEDGKFYDRN
                     GTGLAKGFLRFPTAKQFRISSNFNPRRTNPVTGRVAPHRGVDFAMPQGTPVLSVGDGE
                     VVVAKRSGAAGYYVAIRHGRSYTTRYMHLRKILVKPGQKVKRGDRIALSGNTGRSTGP
                     HLHYEVWINQQAVNPLTAKLPRTEGLTGSDRREFLAQAKEIVPQLRFD"
     gene            complement(28913..29845)
                     /gene="znuA"
                     /locus_tag="ACHKBL_000975"
     CDS             complement(28913..29845)
                     /gene="znuA"
                     /locus_tag="ACHKBL_000975"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HFO5817888.1"
                     /translation="MLHKKTLLFAALSAALWGGATQAADAAVVASLKPVGFIASAIAD
                     GVTETQVLLPDGASEHDYSLRPSDVKRLQNADLVVWVGPEMEAFMQKPVSKLPEAKQV
                     TIAQLEDVKPLLMKSIHDDDDDHDHAEKSDEDHHHGDFNMHLWLSPEIARATAVAIHG
                     KLVELMPQSRAKLDANLKDFEAQLASTETQVGNELAPLKGKGYFVFHDAYGYFEKQFG
                     LTPLGHFTVNPEIQPGAQRLHEIRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMG
                     TLDPLGTNIKLGKTSYSEFLNQLANQYASCLKGD"
     gene            29924..30679
                     /gene="znuC"
                     /locus_tag="ACHKBL_000976"
     CDS             29924..30679
                     /gene="znuC"
                     /locus_tag="ACHKBL_000976"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015633 - ABC-type zinc transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HFO5817889.1"
                     /translation="MTSLVSLENVSVSFGQRRVLSDVSLELKPGKILTLLGPNGAGKS
                     TLVRVVLGLVTPDEGVIKRNGKLRIGYVPQKLYLDTTLPLTVNRFLRLRPGTHKEDIL
                     PALKRVQAGHLINAPMQKLSGGETQRVLLARALLNRPQLLVLDEPTQGVDVNGQVALY
                     DLIDQLRHELDCGVLMVSHDLHLVMAKTDEVLCLNHHICCSGTPEVVSLHPEFISMFG
                     PRGAEQLGIYRHHHNHRHDLQGRIVLRRGNDRS"
     gene            30676..31461
                     /gene="znuB"
                     /locus_tag="ACHKBL_000977"
     CDS             30676..31461
                     /gene="znuB"
                     /locus_tag="ACHKBL_000977"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HFO5817890.1"
                     /translation="MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLL
                     GVAFGLLLDVNPFYAVIAVTLLLAGGLVWLEKRPQLAIDTLLGIMAHSALSLGLVVVS
                     LMSNIRVDLMAYLFGDLLAVTPEDLISIAIGVVIVVAIMFWQWRNLLSMTISPDLAFV
                     DGVKLQRVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAV
                     LVGMVAVTGGLTFSAFYDTPAGPSVVLCAALLFIISMMKKQAS"
     gene            complement(31611..32621)
                     /gene="ruvB"
                     /locus_tag="ACHKBL_000978"
     CDS             complement(31611..32621)
                     /gene="ruvB"
                     /locus_tag="ACHKBL_000978"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HFO5817891.1"
                     /translation="MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIF
                     IKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMGVNLRTTSGPVLEKAGDLAAMLT
                     NLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
                     TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARG
                     TPRIANRLLRRVRDFAEVKHDGTISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKF
                     FGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMATVRAWNHFGITPPE
                     MP"
     gene            complement(32630..33241)
                     /gene="ruvA"
                     /locus_tag="ACHKBL_000979"
     CDS             complement(32630..33241)
                     /gene="ruvA"
                     /locus_tag="ACHKBL_000979"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HFO5817892.1"
                     /translation="MIGRLRGIIIEKQPPLVLIEVGGVGYEVHMPMTCFYELPEAGQE
                     AIVFTHFVVREDAQLLYGFNNKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAV
                     EREEVGALVKLPGIGKKTAERLIVEMKDRFKGLHGDLFTPAADLVLTSPASPATDDAE
                     QEAVAALVALGYKPQEASRMVSKIARPDASSETLIREALRAAL"
BASE COUNT         7969 a         8606 c         8318 g         8457 t
ORIGIN      
        1 caggcacaat ttcttgtgcc tgattgatat tacttgacgg gagcgggttg ttccgcgggt
       61 ctggcttttt caagcttcgc cagatcgagt gcgatattca ccgtctcatc cagataagga
      121 tccggctcct ggtaatcttt cggtagatca tccagtttct tcaactccgg tttaccttcg
      181 cgtttaaagc gttcgttcaa acgcgccaga cgcgtcgcat catcttcatt attctctttc
      241 tcacgcacag cgtaattcag agaaacgata ttgcgcttgt ccttcatggc gttgaagcgc
      301 gcgatatcct tcatgatgtt ctggaactca ggatctttcg cgatacgcgc attatgttcc
      361 ttcagcagct ccggtccaaa ggccgttaaa tcgcctgatt tcacataggt cgcggcatcg
      421 atgctatccc acggcagcgc gttatcttcg aatttctcac ccgtttccgt ttcttcatta
      481 cccgtcggca tgatgatgtc tggcgttaca cctttacgtt gcgtactgcc gccgttaacg
      541 cgatagaatt tctggatcgt gtactgtaca gatcccagcg ctggccattc aggacgtaac
      601 atctgatcgt agatacggtt caatgaacgg tattgctgaa cggtgccttt accaaacgtc
      661 ggttcaccca caaccagcgc acgaccgtaa tcctgcattg ccgcggcaaa gatttctgaa
      721 gccgaagcac tgaaacggtc aaccagtacc accagcgggc ctttatagaa gacctgtccg
      781 tcggtatcgc tgtcttcacg aactttaccg ttgttatcac ggacctgaac aatcgggccg
      841 gatggaataa acagaccgga gagcgatacc gcttcggtca gcgccccacc accattgcta
      901 cgcaggtcga tgatgacgct gctgacattc tgtttttcca gtttctgcag ttgcactttg
      961 acatcgtctg tcaaacccac atagaagccc ggaatatcca gcacgccgac tttctcttta
     1021 ccgacggtct tcaccgacat tttaaccgcg cggtcttcga ggcgaatacg ttcacgggtc
     1081 aacgttacag tacgggtctt ggtcccttta ccagcaggta aaatttccag acgaacttta
     1141 ctgcccttcg gccctttaat taaggcaacc acatcatcaa gacgccagcc aatcacgtca
     1201 accatcggct tgcctgtttg accaacaccg acaattttgt caccaacgct gatagcttta
     1261 ctcttcgctg ccgggcctcc tgccaccatc gaattgataa cggtgtagtc atcatccatt
     1321 tgcagcactg cgccaatgcc ttccagcgac aaactcattt cagtgttgaa ctgttcggta
     1381 ttacgcgggg aaagatagtt ggtatgcggg tcgatttcac gcgcaaacgc cgtcattgcc
     1441 agcgagaaaa catcttcgct gttggtttgc gccagacgac gaatggcaaa tttgtagcgg
     1501 cgagtcaggg tttcacgaat ttctttatcc gtttttcctg ccagcttcag gcttaactcg
     1561 tcgaatttga ctttactgtc ccacagcgcg ttcaactcag cctcgttttt cggccagggc
     1621 gctttgctgc ggtcaaggtt ataagtgtcg ttgccggtga aatccatcgg cttttccagt
     1681 accgacaaag cgtactggta acgttcaaaa cgacgctttt gcgccagatt gtagagatcg
     1741 tagaaaacgt cgagtttgcc tgaacgcagt tcatcgccta actcggtttt ctttttcgcg
     1801 aactgttcaa catcgcttgc caacagcacg ttgtggctgt aatcgagcag attcaggtag
     1861 cggtcaaaga ttttggccga aaatgtctga tcgaggtcga actggcgata atgagaacgg
     1921 gtgaagcgcg acgttacgcg ctcacttacc gtcgcatgct gcgtctcttc ctttaatacc
     1981 ggaatttgat cagcacgcgt gatatcttct acagcgaagg tctggcctgc tattgcaagc
     2041 aggccagcta acgcggtaag cctaaaaaac atgttcatgc ctggcccggc ctccgtttca
     2101 gaacaccagg tgttctgcgc gcacaatcaa agacataccc gaattcagct ggacgcggac
     2161 gccgtctttg gtgatttcta atacggtggc atccatcgcg ttttgacccg ctttcacctt
     2221 cagggcttgt ccgacagtca gagctgaaat gtcagaaacc ggggtgtgct gttcttcgcg
     2281 aggtgctttt actgtttttg gcgctttctc taccggcttt tgcgcacgag gtttacgttc
     2341 agcgccttct ttgcggcgtg gcgtggtcgg acgtggcttg cgttcgcggc gcggtgcgtc
     2401 ttctttctca ccagcagctg cggcagcttc gcgttttttc gcttgctgtt cagcacgctg
     2461 tgcctgaaca cgcgctttcg cttcttcaag ctgcttgcga gcatgctcta catgttgctc
     2521 gtccagctca ccgcatgggt tgccgtcaag atcgacacgc gttgcgccgg gtttaacacc
     2581 gtaaagataa cgccagctcg aagtgtagag acgtaaagcg gatcgcaatt gcgttttgct
     2641 caggttcatt tccccggcaa cacgatcgac caaatcctga aaaataccga ttttcagcgg
     2701 acgcgcttca ccttccgcac tgaaacagtg gggaaaacgt tcggccagaa acgcgattac
     2761 ttctttactg ctattcaact taggttgatt ttccatgaaa tttcctgatt acaacggacg
     2821 tagccaacaa gcgcaggcat gaacaggcgt cattataatg acgctatcag taaatgctac
     2881 gttatccgtt gattatcctg cgacgctcgc aaagaatttt ttgtaatccg tcgttgcaag
     2941 cactttttca agctgtgcca caagctgacg taagccttgc tcgtcctcgt ctgtaaagcg
     3001 accgaagacg gtactatcga tgtcgagaac accaataatc tgatttttca ccaccagcgg
     3061 cagaacaatt tcagaattac tcgccgcatc acaggcaata tgcccgtcaa acacatgaac
     3121 atcctcgata cgctgcactt gattgcgggc aaccgcagtg ccgcacacgc cgcgcccgac
     3181 gggtatccgg acacaggcaa ttttgccctg aaatggtccg agtaccagtg tatcgtcctc
     3241 aagcaaataa aaacctgccc agtttacgtc agtgagacgc tcatataaca acgcactggt
     3301 gttcgcaagc gttgccagaa aactggtttc tcctgccatc agcgcgttaa agtcgcgatt
     3361 taaatccgcg taaaattcta ttttgttcat tatgtaatca cttggttgtc ttaccaggaa
     3421 ctgccagcct attaaaataa gcattaaatg cgttaatgct caagatcatt ccaatcatgg
     3481 gttaagatta atgttaattc tttttacatt tggcacgtca tggctcttaa cacaccacaa
     3541 attacgccga caaaaaagat aacagtgagg gctatcggtg aggaactgcc gcgtggtgat
     3601 taccaacgtt gcccgcaatg tgacatgctg tttagcctgc ccgagataaa ttctcatcaa
     3661 agtgcctatt gtccgcgctg tcaggcaaaa attcgtgacg ggcgcgactg gtcgctaacg
     3721 cgcctggcgg caatggcctt caccatgctg ttgctgatgc cgtttgcctg gggcgaaccg
     3781 ctgttgcata tctggctgtt aggcattcgt attgacgcca acgtcatgca aggcatctgg
     3841 caaatgacca aacagggcga tgcgataacg ggatcaatgg tctttttttg cgttattggt
     3901 gcccccctca ttctggtgac ctccatagct tatttatggt ttggtaaccg actgggaatg
     3961 aatctacgcc cggtactgct gatgcttgag cgacttaaag agtgggtaat gctggatatc
     4021 tacctggtcg gcattggcgt tgcttctata aaggtacaag attatgccca tatccaggcg
     4081 ggtgtcggct tgttctcttt tgtggcgttg gtgattttaa cgacggtgac gttgtcacat
     4141 ctcaatgtcg aagaactgtg ggagcgattt tatccacagc gccccactac gcgtagagac
     4201 gagaaactcc gtgtctgtct tgggtgccat tttaccggct atcctgatca gcgtggtcgc
     4261 tgcccgcgtt gccatatccc gctacgcctg cgtcgccgtc atagtctgca aaaatgctgg
     4321 gcggcgctgt tagcgtcaat cgttttgttg ttacctgcca acctgttgcc tatttctatc
     4381 atttatctga atggtggccg gcaggaagat acgattcttt ccgggattat gtcgctggca
     4441 agtagcaaca ttgccgttgc gggaatcgtg tttatcgcca gtattctggt accgtttact
     4501 aaagtgatcg tcatgttcac tttactgttg agtattcatt ttaaatgcca gcaaggttta
     4561 cgcacacgca ttctgttact gcggatggtg acctggattg gtcgctggtc gatgctcgac
     4621 ctgtttgtca tatctttaac catgtcgctg attaatcgcg atcagatcct cgcttttact
     4681 atgggaccgg ctgcgtttta tttcggcgca gcggtaattt tgactattct tgctgtggaa
     4741 tggctggaca gccgcttact ttgggatgca catgagtcag gaaacgcccg cttcgacgac
     4801 tgaagcgcag attaaaaata aacgccgtat ctcacctttc tggctgctgc cgttcatcgc
     4861 gctaatgatt gccggttggc tgatttggga tagttatcag gaccggggta ataccgtcac
     4921 catcgacttt atgtcggcgg atggtattgt cccgggccgc acccctgttc gttatcaggg
     4981 cgttgaagtc ggaactgtgc aggatatcag cctgagcgac gatcttcgta agattgaagt
     5041 caaggtcagc atcaagtctg atatgaaaga tgcgctgcgc gaagagacac agttctggct
     5101 ggtgacgcca aaggcctcac tggcaggtgt ctccgggctg gatgccctcg tcggtggtaa
     5161 ctatatcggc atgatgccag gtaaaggtaa agagcaggat cactttgtcg cactcgatac
     5221 ccaaccgaag tatcggctgg ataatggcga tctgatgatc cacctgcaag cccccgatct
     5281 cggttcactg aacagcggtt cattggtcta tttccgtaag atcccggtgg gtaaagtcta
     5341 cgactatgcc atcaatccca ataagcaagg cgtggtgatt gatgtcctga tcgagcggcg
     5401 ttttaccgac ctggtgaaaa aaggtagccg tttctggaac gtttccggcg ttgatgccaa
     5461 cgtcagtatc agtggcgcga aggtgaaact ggaaagtctg gcggcactgg ttaacggtgc
     5521 gattgccttc gattcacctg aagagtcgaa acctgccgag gcggaagata cctttggtct
     5581 gtatgaagat ctcgcccaca gccagcgtgg cgtaataata aaactggaac tgccgagtgg
     5641 ggccggatta accgccgact cgacgccgtt aatgtatcag gggctggaag tcggacagct
     5701 gactaaactg gatttaaatc ctggtggtaa agtcaccgga gaaatgaccg ttgatcccag
     5761 cgtcgttacg ctgttacggg aaaatacccg catcgaatta cgtaatccga aattatccct
     5821 tagcgatgcc aatctcagcg ccctgctgac cggaaaaacc tttgagttgg tgcccggcga
     5881 tggtgagcca cgcaaagagt tcgttgttgt gccaggcgaa aaagccctgc tacaagagcc
     5941 ggatgttctg acactgaccc tgacagcacc agaaagttac ggtattgatg ctggtcagcc
     6001 gctcattctc cacggtgtgc aagtaggcca ggttattgat cgtaaactca ccagcaaagg
     6061 cgttaccttt accgtcgcca tcgagcctca gcatcgcgaa ctggtaaaag gcgatagcaa
     6121 atttgtcgtc aacagccgtg tcgacgtgaa ggtggggctg gatggcgttg agtttctcgg
     6181 tgccagcgcc tcagaatgga ttaacggcgg gatacgtatt ctgccgggcg ataaaggcga
     6241 gatgaaagcc agctatccac tgtatgccaa tctggaaaaa gcgctggaga acagccttag
     6301 cgatttaccc accacaaccg taagtttgag tgcagagacg ctgccggatg tgcaggcagg
     6361 atcggtagtg ctgtaccgta aatttgaagt tggtgaagtt attactgtgc gtccgcgagc
     6421 taacgcgttt gatatcgatc tgcatattaa gccggagtat cgcaaccttc tgaccagcaa
     6481 cagcgtgttc tgggcagaag gtggggcgaa agttcagcta aatggtagtg gtctgaccgt
     6541 acaggcatcc ccgctctcca gagcattaaa gggggctatt agcttcgata acctcagcgg
     6601 tgccagcgcc agtcagcgta aaggcgacaa gcgtattctg tatgcttccg aaacagcggc
     6661 ccgtgcggtt ggcgggcaaa ttacgcttca cgctttcgat gccggaaaac tggcggtcgg
     6721 gatgccaatt cgctatctcg gtattgatat cgggcaaatc cagacgctgg atctgattac
     6781 cgcgcgcaat gaagtacagg caaaggcggt actttacccg gaatatgtcc agacctttgc
     6841 acgcggtggt acacgcttct cagtggtcac accgcaaatt tcggcagctg gcgttgagca
     6901 tcttgatact atcctccagc cgtatatcaa cgtcgaacca ggccggggca atcctcggcg
     6961 cgactttgaa ttacaagagg ctaccataac tgattcgcgt tacctggatg gcttaagcat
     7021 tattgttgaa gcgccggaag ccggttcgtt aggcatcggt acgcctgtgc tgttccgtgg
     7081 tctggaagtc ggtacggtta ccggaatgac gctggggaca ttgtcagatc gcgtgatgat
     7141 tgcgatgcgc atcagtaaac gctatcaaca cctggtgcgt aacaattccg tcttctggtt
     7201 ggcatcgggt tacagtctgg actttggtct gacgggcggc gtagtgaaaa ccggcacctt
     7261 taaccagttt atccgtggcg gcatcgcctt cgccacgcct ccgggtacgc cactggcacc
     7321 gaaagcccag gaaggcaagc acttcctgtt gcaggaaagt gaaccgaaag agtggcgtga
     7381 atggggaact gcgcttccca aataatgccc actgctccgg cgtgcctgcg ccggagcgtt
     7441 tatgctaaac tgcgcgcctg tttttttgcc agtggtatat gctcgtggcc caacacaccg
     7501 tttatttccc ggacgccttt ctgacacaaa tgcgcgaagc gatgccttcg acgctctctt
     7561 ttgatgattt tcttgccgcc tgtcagcgcc cgttgcgccg cagcattcgc gttaatacgc
     7621 tgaaaacctc cgttgctgat ttcctgcaat taactgctcc ttatggctgg acgcttacgc
     7681 caattccgtg gtgtgaagaa ggtttctgga ttgaacgcga cagtgaagat gcattgccgt
     7741 tgggtagtac cgccgagcat ttaagcggcc tgttttatat tcaggaagcc agttcaatgt
     7801 tgcctgttgc cgccttgttt gctgacggta atgcaccaca gcgggtgatg gatgtcgctg
     7861 ccgcgcccgg ctccaaaacg acgcaaattg ccgcgcggat gaataacgaa ggggcaatcc
     7921 ttgccaatga gttttccgcc agtcgggtaa aagtgttaca tgccaatatc agccgctgtg
     7981 gcatcagtaa tgttgcgctc acgcattttg atggccgcgt atttggtgcg gcagtgccag
     8041 aaatgttcga tgccattttg ctggacgctc cctgctccgg cgaaggcgtg gtgcgtaaag
     8101 atcccgatgc gctaaaaaac tggtcaccag aaagcaatca ggaaatcgca gcgacccaac
     8161 gggaactgat cgacagcgcc tttcatgcat tacgtcctgg cggtacgctg gtttactcga
     8221 cctgtacctt aaaccgggaa gaaaacgaag ccgtttgcct gtggctgaaa gagacttatc
     8281 ccgacgcagt agagttttta ccgcttggcg atctcttccc tggtgcaaac aaggcgctga
     8341 ccgaggaagg ctttttgcat gttttcccac aaatttacga ctgcgaaggc ttcttcgttg
     8401 ctcgtctgcg taaaactcag gcgattcccg ccttacccac cccaaaatac aaagtgggca
     8461 atttcccgtt cagcccggtg aaagatcgcg aagccggaca aattcgtcag gcggctgcaa
     8521 gtgttggctt aaactgggat gaaaacctgc gactctggca gcgtgacaaa gaactgtggt
     8581 tgttcccggt gggcattgaa gccctgatcg gtaaagtccg attttctcgc ttggggatta
     8641 aacttgccga aacgcacaac aaaggttatc gctggcagca tgaagccgtt attgcccttg
     8701 cctcccccga caatgtgaac gcttttgaac tgacaccgca ggaagcggaa gagtggtatc
     8761 gcgggcgcga tgtttacccg caagccgcgc cagtagcgga tgatgtattg gttactttcc
     8821 agcatcagcc gattggttta gccaaacgga ttggttcgcg actgaaaaac agctacccgc
     8881 gtgaactggt gcgcgatggg aaacttttta ccggtaacgc ctgacagcgc acaaaaaaag
     8941 cgcacttttt gactggcaca ttcggctgcc tcaactaggc tgaaaaatgg tgcgatcgga
     9001 ctggtcgtac cacaaccggc agctaaatgg agagcacgaa gatgaaaacc agtgtgcgca
     9061 taggcgcttt tgaaatcgac gacggcgaat tacacggcga atcgccgggt gatcgaacgt
     9121 taaccattcc ttgtaaatca gaccccgatt tatgtatgca actggatgcc tgggatgctg
     9181 aaaccagtat tcctgccctg ctaaatggcg aacactctgt cctttaccgt acccgttacg
     9241 atcaacaatc tgatgcctgg attatgcgtc ttgcctgatc caaaaagaac ccgtcggcat
     9301 ggcgggttat ttgtcctggt tatttccccg ttgtaaaatc tctcctaaac ttaacggtac
     9361 ggcaccacac ttcggggatg aaatgttcgc gctggtactt tttgtttgct acctggatgg
     9421 cggttgtgaa gatattgttg tggatgtcta caacacggaa cagcagtgtc tttattctat
     9481 gagcgatcaa cggatccgcc atggcggttg ttttccaatc gaggatttta ttgatggttt
     9541 ctggcgacca gcacaggagt acggtgattt ttaattattg caattgcaca agagtcagtt
     9601 cgcccccaaa gacagcaccg gtatcaatat aatgcaggtt gccaatatcc acgcgatgtc
     9661 gcaacggtgt atgaccaaac cagaaatgat cagcaccttt aattccctgc ccttttgggc
     9721 gttcacctaa tcgcgagcgg ctccacaaga cctgatgcaa atcaacgtcc ttttgccatt
     9781 cataaacatc atccggataa tcggcatggg caataacatg tttgccggtg cgactgtgta
     9841 cttcaagaat aaagggcaaa tgctgacatt tttccagcgc cgttttcgct tgtttctgtt
     9901 gattatctgc cagcgcaata aaccagtcgc cgccattcat gaaccacaaa gacatctgct
     9961 gggatgccag cgcatccatc gccatctgtt catgattgcc tcttaccgca cgaacccaat
    10021 gttgttccag taactgcagg caacgtaaac tttgcggccc acgatcgata acgtctccca
    10081 ctgagataag taaatctcgc cacggatcaa aacgacaatg ccataatttg cggcgcaact
    10141 gctcaagaca accgtgtata tcgcctgaaa gccagatatg tcgccattga tgacccgcaa
    10201 ttctctgata aacgggcgca ggctgtttca tcaatatttt cctcccgcgc taaagatcac
    10261 ataatcttaa caagaatgtt aaaaaacgct ggacgcagac agtagagtgt gtgttatggt
    10321 tgactataaa gtcagcgaag ggaatgcttc tggcttttaa cagataaaaa gagaccgaac
    10381 acgattcctg tattcggtcc agggaaatgg ctcttgggag agagccgtgc gctaaaagtt
    10441 ggcattaatg caggcttagt tgccttgctc tttaagaata gatgacgacg ccaggttttc
    10501 cagtttgcgt gcaaaatggt caataaaaag cgtggtggtc atcagcttaa atgttaaaaa
    10561 ccgcccgttc tggtgaaaga actgaggcgg ttttttattg gaaatcaaaa ggctatttta
    10621 ggtaattaac agagtttttc agctcgctct ataaacggtg ccagactcat tttttcgccg
    10681 ggattgttag gatcatcaat ctgaatcacc gaaatgggtt gggctttagt cttcccactg
    10741 gcaacttcct tttgtgcgat atcgtttaaa ggatactgca cgagggtact tgggttgatg
    10801 acatacaaag cattacccgg tcggcaagtc agcatcacct cttcgcgatt aaacgcccat
    10861 ttgtctttac ccacttcaaa acggctgacg gtaatcacct gcggtgccgc cagcgccgct
    10921 gcagaactgg tgagtaacag aaacgccaga atactttttt tcatcatttt cgtaatccat
    10981 caaaaaggtt cgagagtcgc aagcaggctg atgatcacca gcactaccgc acctatcgcc
    11041 cattcgagtt tagtcatcca cacaaaccag ggagccgcac gatcttcatc ctgcctcatg
    11101 cgcggtacaa gaacataacg attagccagc gcgatgacca ccataatcat caccaggatg
    11161 gctttgagca ttaagagttg gccccagtaa gtcgtaagcg tggggggaaa tccggtaatc
    11221 aacaatgcat taagcacgcc gcttgccagt acgccgatca ccgcaaaatg cccgcaccag
    11281 gaaaaacgca tcagcgcctg aatagcctgg tgtcgccaac ggcctttgat gagttgcata
    11341 caccagagca ccggcagtaa tcccccaaac cacgccgccg cacagatcag gtgaattgcg
    11401 tgattagtct gatggatttt cgccgttacc ccttcattca gcgtcgcatg cccgactccc
    11461 gccagcagaa taaattgcgc ggtagtgagc ataaatagca aacgtggcat attctgcggt
    11521 tgcataaggg caacgatcaa cgttacgagg gcgagaacaa tttgccataa ccagatgcca
    11581 ccaaactgcg tttgcaaaac agccagccaa atgtttggcg aaaatacatc tgaccatccc
    11641 gttcccatca acccgccctg aacggcgagc attgcagtgg cactgatcaa actccagacg
    11701 gcagcgtgct gttgtaaacg cagaaaacgc ttcgtcagca agcgacgaat cgttaagggg
    11761 gccagccagg caccgtacat tgcgaagcca aaaaccagca tcaacgaggt gaaatggata
    11821 aatcgcagcg cgatccaggt aaacgccagc atgatttatt tcacgctaaa ggtgtagtgt
    11881 cctttcgttt tatgcccgtc caccgaaaca acatgccagt caacggtgta ggttccgggt
    11941 ttcagcgaat cggccagtgg aacaatcagt tgcttttggt cctgctcatt ccgcttcgcc
    12001 ggtaatgttt taatattttc gttttttggc cccgtgattt ttgcaccact gaatcctgtt
    12061 tcaacgcctt ccgagaagtt taaagtgatt gcctgcgggg cagctgtcac ttgcgcattt
    12121 gccgcaggat actgatgcgt taaatgcgca tgtgcccaga cggaaggtgt caccaacgaa
    12181 gtggtcagga ttgccagcgc gtagcgaagg gagcgtgcag ttgaagccat attatctatt
    12241 cctttttgta ataacttttt tacagagcat aaccttgtct aatgtctgag tcgaggatca
    12301 tcaattccgg cttgccatcc aggctcactc ttagtaactt ttgcccgcga atgatgagga
    12361 gattaagaat gctgaagaat ctggctaaac tggatcaaac agaaatggat aaagtgaatg
    12421 tcgatttggc ggcggccggg gtggcattta aagaacgcta caatatgccg gtgatcgctg
    12481 aagcggttga acgtgaacag cctgaacatt tgcgcagctg gtttcgcgag cggcttatcg
    12541 cccaccgttt ggcttcggtc aatctgtcac gtttacctta cgagcccaaa cttaaataaa
    12601 acttatacag agttacactt tcttacataa cgcctgctaa attatgagta ttttctaaac
    12661 cgcacccata atttgcagtc attttgaaaa ggaagtcatt atgtcgttct ggaaagttgc
    12721 agcagcgcaa tatgagcctc gtaaaacctc actgaccgaa caggtagctc atcatctgga
    12781 gtttgtgagt gccgccgcca ggcaacaatg tcagctatta gtttttccat cgctttcttt
    12841 actggggtgt gattattcgc gacgtgccct tcctgcccca cccgatctct cgctgttaga
    12901 cccgctttgt tatgctgcaa cgacctggcg aatgaccatc attgccggcc ttcccgttga
    12961 atataacgat cgctttattc gtggaattgc ggtgttcgca ccgtggcgaa aaacaccagg
    13021 gatctaccat cagagtcatg gtgcatgtct gggccgtcgt tccagaacca tcacggtggt
    13081 cgatgaacaa ccggaaggta tggatatgga cccgacctgt tcactgttta cgacagggca
    13141 atgtctgggg gaacccgacc tactggcatc tacccgccgc ttacaatttt tttcacatca
    13201 gtactcaatt gccgtgctga tggcaaatgc ccgtggtaac agtgcactat gggatgaaca
    13261 tggtcgtctc atcgttcgcg ccgatcgtgg ttcattattg ttagtcggtc agcgttcatc
    13321 acagggttgg caaggcgata tcattccatt acgctaggct ttttcggcct ggagcatgcc
    13381 atgttgcgca ttatcgatac agaaacctgc ggtttgcagg gagggatcgt tgagattgcc
    13441 tctgttgatg tcattgacgg aaaaatcgtc aaccccatga gccacctggt gcgccccgat
    13501 cgtcctatta gtccacaagc gatggcgatt catcgcatca ccgaagccat ggtcgccgat
    13561 aaaccgtgga ttgaagatgt gatcccacac tattacggta gtgaatggta tgtcgcgcac
    13621 aacgccagct ttgaccgccg cgtactgcct gagatgcccg gtgagtggat ttgcactatg
    13681 aaactggccc gtcgtttgtg gccagggatc aagtacagca atatggcgtt atataaaaca
    13741 cggaagctca atgtacagac gccgccgggc ctgcatcatc accgcgcgtt gtatgactgt
    13801 tatatcaccg ctgcgttgct tatcgatatt atgaacacct ccggctggac ggcagaacag
    13861 atggccgata tcaccggacg tccgtcgttg atgacgacct tcacctttgg caaataccgt
    13921 ggcaaagcgg tttccgacgt tgccgaacgc gatccgggct atctgcgctg gttgtttaat
    13981 aacctggaca gcatgagccc ggagctgcgt ttaacactga aacattatct ggaaaatact
    14041 taatcctcag gcgtagcggg taatgttccc tgcgccagcg cgaccagaaa agcatattcc
    14101 atcgctacgc cttcgtacga tttaaagcga ccagatttac cgccatgacc tgagtccatg
    14161 tcggtacaga gcaataaaag atggtcatcg gttttcagct cgcgcaattt agcgacccat
    14221 tttgccggtt cccaatattg cacctgagaa tcgtgcaaac cggtcgttac cagtaaatgc
    14281 ggataagcct gtgcggtgac gttgtcatat gggctgtagc ttttcatgta ctcgtaatat
    14341 tgcggatcct gcggattccc ccactcttca aactcaccag tggtaagagg aattgattca
    14401 tcaagcattg ttgttacaac atcaacaaac ggtacctggg cgataacgcc gtggaataat
    14461 tccgggcgtt gattaattgc aacgcccatc aacatgcccc ccgcacttcc gcccatcgca
    14521 taacagagcg aaggagagcc atagcccagt tttaacaatg catcgcaggc atcaagataa
    14581 tcattaaacg tatttttctt cttcagaaat ttaccgtctt cgtaccattg ttgccccagc
    14641 tcaccaccgc cgcgaacatg gacaatggcg tagacaaagc cacgatctaa caaactcaag
    14701 cggctaaaac tgaaatcggc atcaatactt gcgccgtaag aaccgtagcc atacaccagc
    14761 agcgggttgt gtcctttgcg aaaatgtttg cgatgataga ccagcgaaac cggaacttcg
    14821 acgccatcac gggcgactat ccacaggtgt tcactgcggt aattcgccgc atcaaaacca
    14881 ggaacttccg tttgttttaa tacacgacgc tcaccggtat ccatatccag ttcaaacaaa
    14941 gtatctggtg tggtcatgga agaataacca taacgcaatc gcgcggtttc aggttctgga
    15001 ttgtaggcaa tccaggtcac ataggccgga tcatcaaagg caatgccaat gacttcccgg
    15061 gtcttgcggt tgatttggcg caaactggtt aacccgcgct gacgctcttc aaccaccagc
    15121 cagtcggtaa acagcgtaaa cccttccagc ataatgttat cacgtggcgg aattaactct
    15181 tcccactgtt gctcatcacg catacgggta cggtataagc caaagttttt accgtggcgg
    15241 ttggaacgca gataaaaccg atgctggtag tgatcaaggc tgtattcgtg atctttgcgg
    15301 cgaggcagaa aaacaaacgg ctcggcatcg gccatttccg cgtccagcag gcgaacttca
    15361 ctggtggtgg cgctggccag atgaatgacc acataatgct tcgaggtcgt tttatgcagg
    15421 ctgacgtaat aggtatcgtc tttttcttcg taaatcagtt tatcttgcga tgctggcgta
    15481 ccgatggcgt gacgccagac ctgataaggc agcagcgtca ctggatgctt gcgaacatag
    15541 tagaaagtcc aggagtcatt tgcccagaca aagctgggtt caacgttatc cagcagttcc
    15601 gggtaccagt tacctgtttc cagattacga aaacgaatgc catactgccg tcgggaaaga
    15661 aaatcttctg ccagtgccat aatggtgtta tctggcgtaa tcgccattcc gcccatcgaa
    15721 taaaactcac tatgagccgc gcgcttgttg gcatcgagca atgtttccca ctcgtcccac
    15781 tcttcactga acgccgattg acgctggtag atagcatatt cacagcctgg ttcataaata
    15841 tgccgatagc ggtagccatt tttgatgtag ggcgcagaaa cttctcgttg cggaatgcgg
    15901 tcgatgattt cctttaagat gcgatcctgc aaggcttgtt gcgaggccat cacccgatga
    15961 ccgtaactat tttcttgttg cagatagtcc aggactttcg gctgagaacg cgtatcgtcc
    16021 cgcagccagt agtaattatc gatgcgcgta tcgccatgaa gcgtcatggc gtggggaatg
    16081 cgggcggctt ttggtagcat gttattgttc tttctggttg aaacatctta taagggtggc
    16141 aaaactcacc ggggatgcaa gcgaaacagg gtagtcattg cttagatgat gacaggtaat
    16201 ggcgcggata tcgaatgtta tgcagacaga gaaaatcagc ctgctcaaat ggctgtgcgg
    16261 ttctggataa cccgaaataa tcagcttcag gctatccaga gagcggaatt actccgccaa
    16321 agtacgtttt tgctgttcga gatcgcgttc aatgccttca cgaacatcct gggggatttt
    16381 cagcgcatca cccagtgcat tcaggtaact gcgttccata aaatggtcaa tatcaatagc
    16441 cgcgcagctc aggaaataga tttccagcgc ctcttcttca ttgcggaccc cggtagccag
    16501 gcgttgtgga tccagcggtt gttcgattgc ctgctcaatg agtacacgcc ccttctcttc
    16561 cacaccggct tctcgcaatt gctggtcgat tgccgcacgt tctttggcat caatatggcc
    16621 atcactttta gcggcaaaga ccagcgcaag gatcaaacgt tccgtacgct catccagcgg
    16681 cgtactttgc gcgccaaact gcggttcatc ctgatgcgcc gcgcgaattt tatctttgta
    16741 tttattccac agcaccgtac ccgctaccgc tccgccgcca accagtaacg cgtttgtgcc
    16801 atatttcgta agaagtttac gtgctgattt atttgcgacc agcagcccag ccagtccgcc
    16861 taatgcgcca ggcactaaaa gtttgcccaa tccctgatcc gcagacgagg aggtagaaga
    16921 acggctttgc ccaagcaggg attgcagttg atttaaccag ttagccatat ttgctcctca
    16981 ataaccattt ttagtgcctg tagcatagcg gatgaggatg tcagaaaatg tatattgaga
    17041 caaaagatgc gcaaataagc ggggcggtag gccgttattc aaagaaattc gcgccaggta
    17101 aggtgttcac tgtatataag gtgataaggg tattttaggt gaaagtagtt tgacgttttg
    17161 agtggaactg tttgatgctg attgcccggt tcgacgctgc tgcgtcttct caggactacg
    17221 attgtgttgc gttgtagggc ggacaaggca ctcgcgccgt atccgccaac ggtcaatcga
    17281 attatcagaa gccattaacg ccgctgatat tccgccattc ccgctttgca gtccacgctg
    17341 acctggtaat gaatatcggc acttttacca cgtacggtta gcggtactga ccatttatca
    17401 tctttcccct gaatgtcctg caaactgacc catgccacgg gatcgggctg accgacaatt
    17461 ttttgatcat cagcccagcg cgccacgcga ttttgttggt aatcacgttt tacgctcgcg
    17521 gcaattccgg cagcatccag accttcacac tttgggaaag tgaccgactt gctggtttca
    17581 ttattggcag cgaaaactga tgcgcaggca gaaaccaaca acagccctaa aaacgcccct
    17641 ctttttttca tgtttttctc catagcacaa tgattcagga gaaagcatgg tacaaattgt
    17701 caggagcgca agttgcttca ggccgcgtgt gaggcatctt cctcttcgga ttcagcaccg
    17761 acgacctgtg gggacggtaa tttgccggtt ttcagaatgg tgctcagcac atctttttgt
    17821 tctgccagcc atagcgaaag ggcttcacgt tgctcgtctt ccatttgcac tggagaattt
    17881 gtcagccagg tgtcaagcag atccgccgta tcaagcattt tgtcataagc atcggcttcc
    17941 tttttgctgg taaacgacat tttctcttcg ccctcacgaa tgactacgta tttaacttca
    18001 accgccattt gcagcctctc ataataactg tgattttata cagtatattt cttctcgatt
    18061 gggaaatcaa catcaacaat aaagacacgc gcaaacgttt tcgtttatac tgcgcgcgga
    18121 ataaatcagg ggatattcgt tatgacgtta ttaggcactg cgctgcgtcc ggcagcaact
    18181 cgcgtgatgt tattaggctc cggtgaactg ggtaaagaag tggcaatcga gtgtcagcgt
    18241 ctcggcgtag aggtgattgc cgtcgatcgc tatgccgacg caccagccat gcatgtcgcg
    18301 catcgctccc atgtcattaa tatgcttgat ggtgatgcat tacgccgtgt ggttgaactg
    18361 gaaaaaccac attatatcgt gccggagatc gaagctattg ccaccgatat gctgatccaa
    18421 cttgaagagg aaggactgaa tgttgtcccc tgcgctcgcg caacgaaatt aacgatgaat
    18481 cgcgagggta tccgtcgcct ggcagcagaa gagctgcagc tgcccacttc cacttatcgt
    18541 tttgccgata acgaaaacct tttccgcgag gcggttgctg aaatcggcta tccctgcatt
    18601 gttaaaccgg tgatgagttc ctccggcaag gggcagacgt ttattcgttc tgcagagcaa
    18661 cttgctcatg catgggagta cgctcagcaa ggcggtcgcg ccggagcggg ccgcgtaatt
    18721 gttgaaggcg tcgttaagtt tgacttcgaa attaccctgc taaccgtcag cgcggtggat
    18781 ggcgtccatt tctgtgcacc ggtaggtcat cgccaggaag atggcgacta tcgtgaatcc
    18841 tggcaaccac agcaaatgag ccagctggct ttggaacgtg cgcaagagat tgcccgtaaa
    18901 gtggtgctgg cactgggcgg ttatgggctg tttggtgtcg agctgtttgt ctgtggtgat
    18961 gaggtgattt tcagtgaggt ctcccctcgc ccacatgata ccgggatggt gacgttaatt
    19021 tctcaggatc tctcggagtt tgccctgcat gtgcgagcct ttctcggact tccggttggc
    19081 gggatccgtc agtatggtcc tgcagcttct gccgttatcc tgccacaact gaccagtcag
    19141 aatgtcacgt ttgataatgt gcagaatgcc gtaggcgcag gcatacagat tcgtttattt
    19201 ggtaagccgg aaattgatgg cagccgtcgt ctgggtgttg cgctggcgac cgctgagagt
    19261 gttgatgaag cgattgagcg cgcaaagcac gccgccggac aggtaaaagt acagggttaa
    19321 acccggcaaa aaaacgctac aaaaatgccc gatcctcgat cgggcatttt gacttttaca
    19381 gcttagcgcc ttctacagct tcacgcgcca gcttagtaat gcggtcgtaa tcgcccgctt
    19441 ccagcgcatc tgccggaacc agccaggaac caccgatgca cagcacgctt ttcagcgcca
    19501 ggtagtcacg gtagttagcc ggagaaatac cacctgtcgg gcagaaacgg acctgggaga
    19561 acggacccgc gatcgcctgc agggctttca caccgccgtt agcttcagcc gggaagaatt
    19621 tgaactcttt caaaccgtag tccataccca gcatcagttc ggaaacagtg ctgatccccg
    19681 gaatcagagg aatagtccct tcggtagcag ctttcagcag cggctcggtc agacccgggc
    19741 taattgcaaa ctgtgcaccc gcttcagtga cttctgccag ctgctgtgga ttcagcaccg
    19801 taccggcacc cacaatcgct tcaggcactt ctttggcgat agcacggata gcgtcaactg
    19861 cgcactcggt acgcagagtc acttccagaa cgcgcacccc accagcaacc aacgcttttg
    19921 ccatcggcac cgcgtgttcc agttttttta ccacgataac cggtacaacc gggccggtgg
    19981 tcaggattga ttctgcactt gttttccagt ttttcatcag agttttctct cgcctgatta
    20041 caaatttgtc gtcttaaaaa gtgatacagg ttgcgccctg ttcggcaccg gacagttttt
    20101 cacgcaaggc gctgaataat tcacgtcctg ttcccacgcg tgacgcgctc aggtcaggaa
    20161 tgtgtggttc gcgagcagcc agttccgctt cgtctaccag cagcgtcagt tcgcctgtct
    20221 gtccattcac acgaatgatg tccccgtcgc gcacttttgc cagcagcccg ccatcgtagg
    20281 cttctggtgt tacgtggata gctgacggca ctttacctga agcgccggag agtcgtccat
    20341 cagtaactaa cgcaattttg aaacaccggt ccaataatac accaagtggc ggcatgagtt
    20401 tatgtaattc tggcattccg ttcgcttttg gcccctgatg acggacaaca acgacacaat
    20461 cgcggtccag caaacccgct tcaaaggccg gcataacgtc atgctggctt tcaaaaacaa
    20521 ccgcaggcgc ttcaatcacc tggttctcta ccggcacggc agaggttttc ataaccgcac
    20581 ggcccaggtt accgcttaac acttttgtcc caccatgatg agagaaaggt tgttcgaagg
    20641 aagcgatcac attgctgtcg agtgattttt ccgccccttc ccgccagtcc agttcaccat
    20701 tattcagcca tggttcaagg gtataacgag acagaccaaa gcctgccacc gtattgacat
    20761 cttcatgcag caggcctgct ttgagcagtt cacgcaccag aaccggtacg ccacctgccg
    20821 cctggaagtg gttaatatcg gccggaccgt tcgggtagag acgtgccatc agcggtacaa
    20881 catcagaaag gtcagagaag tcatcccagt taatctgaat accggctgcg cgcgccatcg
    20941 ccaccaggtg catggtgtgg ttagtggaac caccggtcgc cagcagtgca acgataccgt
    21001 tcaccaccac tttctcatcg atcatcttac cgatcggcat ccattcatta ccattaccgg
    21061 tcatgcgcgt aacctggcgc gcagctgcag cggtcaaagc atcgcgcagc ggagaatccg
    21121 gatgaacaaa agaagagcct ggcaactgca tccccataaa ctccaccacc atctggttgg
    21181 tgttggcagt accgtagaaa gtacatgttc ccggcgcatg ataagacgcg gcttctgact
    21241 ccagtagtgc catgcggtcc actttacctt cggcataaag ctggcgaata cgcacttttt
    21301 ctttatttgg caaaccgctt gccatcggtc cagacggcac aaacaccgca ggcaaatgcc
    21361 caaacgacag ggctgccatc gtcagacccg ggacaatctt gtcgcacaca ccgaggaaca
    21421 gagcaccatc aaacatgtta tgggacagcc ccaccgccgc agacatcgca atcacttcgc
    21481 ggcttaacag cgacaattcc attccatcct gcccctgggt gacaccatca cacatcgccg
    21541 gaacaccgcc tgcaacctga ccaaccgcat ttgcttcatg cagagcttta cgaatgattt
    21601 ctggatagtg ttcataaggc tggtgcgcgg agagcatgtc gttataggag gtgatgatgg
    21661 cgatattgtt acgcaacatg cttttcaaag aggctttgtc ttctggctgg caggcagcga
    21721 aaccgtgtgc caggttaccg catgccaact gcgaacgatg aacggtcgaa gttttcgctt
    21781 gttctatccg ggcgaggtaa gcagagcgag tctcgcgcga acgttcaatg attcgatttg
    21841 ttacgcgtaa caattgtgga ttcataaagg ctcctgaaat tgagttgtca gagcaggatg
    21901 attcacaaca cgtttcattc agaggattca tgactgaaac gcctgtaacc ggagctcata
    21961 gggcaaaaac gtttcagtca gtgtaataaa aaaagcctcg tgggtgaatc cgcacgaggc
    22021 ctgaaagtgt aaaaattgtt ctacaatctg tgcaagatca tgttaccggt aaaataacca
    22081 taaaggatga gcgcagatat tactcaaact cattccagga acgaccatca cgggtaatca
    22141 tcgccactga ggcaacgggt ccccaggttc cggcctgata cggtttcggc gcatcattgt
    22201 ccatcgccca cgcctcagta atggagtcta cccatttcca ggcttcttcc acttcatcgc
    22261 gacgtacgaa cagtgcctga ataccacgca tggtttccag cagcagacgt tcataggcat
    22321 ctgccagatg cgtttgatta aaggtttctg aatagctcag atccagcttg gtgatttgca
    22381 ggttatgttt atggtcaagg ccaggaactt tatttagtac ctggatatcc acgccttcat
    22441 caggttgcag acggatagtc agtttattct gcggcagatc ctgccacgac tctttaaaca
    22501 gattcagttc aggtgttttg aaatagacca cgacttcaga acatttggtc ggcagacgtt
    22561 taccagtacg caggtagaat ggcacaccgg cccagcgcca gttatcaatg tcgacgcgga
    22621 tcgccacgaa ggtttctgta ttgctgctct tgttcgcgcc ctcttcttcc agatatcccg
    22681 gcactttttt gccctgggcg aagcccgcag tatattgccc gcgtacggtt ttttcgcgca
    22741 cgttggagcg gtcaatgcgg cgcagagact tcagtacttt cactttttca tcgcggatgc
    22801 tgtctgcact caggtcagac ggcggagaca tcgcaatcat gcaaagaatt tgcagcaggt
    22861 ggttctggat catgtcgcgc atctgaccgg ctttatcaaa atagccccag cgcccttcga
    22921 tccccacttc ttctgccacg gtaatctcaa catgatcaat ggtgcgattg tcccagttat
    22981 tcacaaacag ggagttagca aaacgcagcg ccaacaggtt cagcaccgtt tctttaccaa
    23041 gatagtggtc gatacggtaa acctggcact cctcgaagta ttcgccaacc tgatcgttga
    23101 tttcctgcga ggtcgccagc gacgtcccca gcggtttctc catgactacg cgtgccggtt
    23161 tagcattcag ttttgcctca ccaagccctt tgcaaattgc gccaaaagtg ctgggcggca
    23221 tggcaaagta gttaatggtg atacgatttt tttgatccag catcgcgccg agacggctga
    23281 atgcagcagt gtcattgaca tcgagattac aaaaatccag acgtgcactc agggtgtccc
    23341 ataaaccttc atcaatggtt tctttcatga aagtttcgag cgcctcgcgg acaactttgg
    23401 tataagccgc tttatcccag tcagcacgcc ctacgccgat aatccgggtg tccgggttga
    23461 gctgaccggc tttttccagt tgatacaggg aaggcagcaa tttacgacgc gcaaggtcgc
    23521 ctttcgcgcc gaaaatgacc aggtcacagg cctgggctgt ttgcgttacc gccatgtcat
    23581 tctccttaag ttaactatcc cggtacttaa gccagggtat acttgtaatt ttcttacggt
    23641 gcactgtact gcttttacga gcttgcgaaa actgtaaacg cttatccacc cgtgcgatta
    23701 cggtaaaagc gcgcaaagtg cggcaaaact gataaaaaaa tcatcgtttt ccattgggta
    23761 aaaatctgac actgatcatg ttatgaaaaa aaataacaac ttttttatct gcttttgtca
    23821 ttaacacagc acacaggcgt aatatctgac aaaactgcat ttcgatttct ttcagagcgg
    23881 aaatcgtcat tacccgtgag tctctttaca tcatgaatat gctggaaaaa atccagtctc
    23941 agctggaaca tttgagcaaa tctgagcgca aagttgccga ggtcattctg gcttcgcccg
    24001 ataacgcgat ccattcgagt attgctgctc tggcgctgga agccaatgtt agcgaaccga
    24061 cggtgaatcg tttctgtcgc agcatggaca ctcgcggttt tcctgatttt aaacttcatc
    24121 tggcacaaag tctggcgaat ggcactccct atgttaatcg caacgtcaat gaagatgaca
    24181 gcgttgaatc atacacgggg aaaatatttg agtccgcaat ggcaacgctc gatcatgtcc
    24241 gtcattcact ggataaatct gccatcaacc gcgccgtcga tttgctcact caggcaaaaa
    24301 aaatcgcctt tttcggatta ggctcttcag ccgccgttgc ccacgatgcg atgaataagt
    24361 tctttcgttt taatgttccg gtggtgtact ccgatgatat cgtgctgcaa cgcatgagtt
    24421 gtatgaattg tagcgacgga gacgtggtgg tgctgatttc tcacacggga agaacaaaaa
    24481 atctggtcga gctggcgcag ctggcacgcg aaaacgacgc catagtgatt gccctcacct
    24541 ctgcgggtac cccgctcgcc cgggaagcaa cgctggcaat taccctcgac gtaccggaag
    24601 atactgacat ttatatgccc atggtttctc gacttgcgca gctgaccgtg atagatgtgc
    24661 tggcgacagg atttactttg cgacgcggtg caaaattcag agataacttg aagcgggtca
    24721 aggaagcgct gaaggaatcg cgttttgata agcagctact taatttaagt gacgatcgct
    24781 aaaaacgact gtcactgtcc taatcttata cgacatccga atgagattaa tttatcgcca
    24841 tcgcggcgtt atttcattcg gatttcatgt tcaagcaaca cctggttgtt tcagtcaacg
    24901 gagtattaca tgtccagaag gcttcgcaga acaaaaatcg ttaccacgtt aggcccagca
    24961 acagatcgcg ataataacct tgaaaaagtt atcgcagcgg gtgccaacgt tgtacgtatg
    25021 aacttttctc acggctcgcc tgaagatcac aaaatgcgcg cggataaagt tcgtgaaatt
    25081 gccgcaaaac tggggcgtca tgtggctatt ctgggtgacc tccaggggcc caaaattcgt
    25141 gtatccacct ttaaagaagg caaagttttc ctcaatattg gggataaatt cctgctcgac
    25201 gccaacctgg gtaaaggtga aggcgacaaa gaaaaagtcg gtatcgacta caaaggcctg
    25261 cctgctgacg tcgtgcctgg tgacatcctg ctgctggacg atggtcgcgt tcagttaaaa
    25321 gtactggaag ttcagggcat gaaagtgttc accgaagtga ccgtcggtgg tcccctctcc
    25381 aacaataaag gtatcaacaa acttggcggc ggtttgtcgg ctgaagcgct gaccgaaaaa
    25441 gacaaagcag acattaagac tgcagcgtta attggcgtag attacctggc tgtctccttc
    25501 ccacgctgcg gcgaagatct gaactatgcc cgtcggctgg cacgcgatgc aggatgtgat
    25561 gcgaaaattg ttgccaaggt tgaacgtgcg gaagccgttt gcagccagga tgcaatggat
    25621 gacatcatcc tcgcctctga cgtggtaatg gttgctcgcg gcgacctcgg tgtggaaatt
    25681 ggcgatccgg aactggtcgg cattcagaaa gcgttgatcc gtcgtgcgcg tcagcttaac
    25741 cgcgcagtaa tcacggcgac ccagatgatg gagtcaatga ttactaaccc gatgccgacg
    25801 cgtgcagaag tcatggacgt agcaaacgcc gttctggatg gtactgacgc tgtgatgctt
    25861 tctgcagaaa ctgctgctgg tcagtatccg tcagaaaccg ttgcagccat ggcacgcgtt
    25921 tgcctgggcg cggaaaaaat cccgagcatc aacgtttcta aacaccgtct ggacgttcag
    25981 ttcgacaatg tggaagaagc cattgccatg tcagcaatgt acgcggctaa ccacctgaaa
    26041 ggcgttacgg cgatcatcac catgaccgaa tcgggtcgta ccgcgctgat gacctcccgt
    26101 atcagctctg gtctgccaat tttcgccatg tcacgccatg aacgtacgct gaacctgact
    26161 gctctctatc gcggcgttac gccggtgcac tttgatagcg ctaatgacgg cgtagcggct
    26221 gccagcgaag cggttaatct gctgcgcgat aaaggttact tgatgtctgg tgacctggtg
    26281 attgtcaccc agggcgacgt gatgagtacc gtgggttcta ctaataccac gcgtatttta
    26341 acggtagagt aagtacgttg ccggatgcgg cgaaaacgcc tcatccggcc tacagttcaa
    26401 tgataattca acagatttcg aatcttctga agcaaacttg aacttatcac cagtcgaagg
    26461 cctctcctcg cgagaggcat tttttatttg atgggataaa gatctttgcg cttatacggt
    26521 tggatttcgc ccggtttgcg agttttcagc aattttaata tccaggtgta ttgttctggt
    26581 cgcggaccaa caaaaatctc gacttcttca ttcatccgcc gcgcaatcgt atgatcatcc
    26641 gcctctaaca gatcatccat cggtgggcgc acctgaatcg tcaaacgatg cgttttgcca
    26701 tcataaatcg gaaacagcgg tacaacgcgc gcacggcaca ctttcatcaa acgaccaatc
    26761 gcaggcaacg tcgctttata ggtggcaaag aaatcaacaa attcgctgtg ctctgggcca
    26821 tgatcctgat cgggtaaata atatccccag tagccctgac gtaccgactg gatgaatggt
    26881 ttaataccat catttctcgc atgcagacga ccgccaaagc gacgacgcac cgtgttccag
    26941 acataatcaa aaaccgggtt gccctgatta tggaacatcg ctgccatttt ctgcccttgc
    27001 gaggccatca gcatggcagg aatatcgacg gcccaaccgt gcggcaccag aaaaatcact
    27061 ttctcgttat tacgccgcat ctcttcgata atctccagcc cttgccagtc aacgcgcggc
    27121 tgaattttct ccggcccgcg tattgccaac tcagccatca ttgccatcgc ttgcggcgcg
    27181 gtggcaaaca tctcatctac aatcgcttcg cgttcagctt cactacgttc tggaaagcag
    27241 agcgacagat tgattaacgc acgacggcgt gagctttttc ccagtcgtcc ggcaaaacgt
    27301 cccagccgtg ccagaatggg atcacggaac tttggcggcg ttaaagcgat acccgccatc
    27361 gcggctacgc ccagccatgc tccccagtag cgcgggtggc gaaaggattt atcaaactca
    27421 ggaatgtatt cgctattatt ttttttcgtt tccatgcttt tccagtttcg gataaggcaa
    27481 aaatcaatct ggtgatagtg tagcggcgca acttgccccg caccaaataa aaaaagccgg
    27541 tactgactgc gtaccggctg cgaatggatg ttaattaatc aaaccgtagc tgcggcacaa
    27601 tctctttggc ctgcgccagg aattcgcgac gatcggagcc ggtcagccct tcggtacgcg
    27661 gcagttttgc cgtcagcggg tttacggcct gctggtttat ccatacttca tagtgcagat
    27721 gcggcccggt tgaacgtccg gtattaccgg aaagcgcgat acggtcgcca cgtttcacct
    27781 tctgtcccgg tttcaccagg atcttgcgca agtgcatata acgcgtggtg tagctgcgac
    27841 catgacgaat agccacataa taacctgctg cgccactgcg tttggcaacc accacttcac
    27901 cgtcacccac tgaaagcact ggcgtacctt gtggcatggc aaaatcaaca cctctgtgtg
    27961 gcgcaacgcg accggtcacc ggattagtac gacgcgggtt aaagttagat gagatacgga
    28021 actgtttcgc cgtcgggaat cgcaagaatc ctttcgccag accagtaccg ttacgatcgt
    28081 agaatttgcc atcttcagcg cggattgcgt aataatcttt accttctgaa cgcaaacgta
    28141 cgcccagcag ctggctttgc tcacgtttac catcaagcat ttctcgtgac attaacaccg
    28201 caaattcatc gccttttttc agtttgcgga aatccatttg ccactgcatg gctttaatca
    28261 ctgcgctcac ttcggcgctg gttaaaccgg cgtttctggc gctggcaaca aagcttcccc
    28321 cgacggtacc tttcagcaga ttgttgaccc actctccttg ctgcatttcg ctggtcattt
    28381 taaaaccgtt agcggcagta cggtcatagg ttcgggtttc acgacgagac acttcccagg
    28441 tgaggcgctg cagttcgccg tccgcggtta atgtccagga gagttgttga ccgattttca
    28501 ggttacgcaa ttctttgtcg gcagcagcca gttgggtgat atcacccata tcaataccat
    28561 actgattgag aatgctgctt agcgtatcgc cagtggaaac aacatattca tgcacgcccg
    28621 cttcaccggc gattttgtca tccagttcat cctggggaat ggcttcatct tcttgtgcag
    28681 cttgatcaat cggctcactg gcttcaggta agagcgaacg aatttcgttc tgttccagct
    28741 caatggtttt gacaattggc gtggcatcgc ggtgataaac atagggccgc cagacagcga
    28801 cggccagagt aagaacggtg agcgacccta acataacgcg gtgtggtcgc ggtaaattat
    28861 taaacgccag ggcgacagag cgggctatct gttgcacgta atcacttcct cattaatctc
    28921 ctttcaggca gctcgcatac tggttggcta attgattcag gaattctgaa tagcttgttt
    28981 tacccagttt gatattcgtc cccaggggat ccaacgttcc catacgaacg gatgtccctc
    29041 gtgcgacgct ctcaacgacc gctggcctga actgtggctc agcaaaaacg caggttgctt
    29101 tttgctcaac caactgtgtt cttatttcat gtaaacgctg cgcgccaggt tgaatctcag
    29161 ggttaacggt aaaatgacca agcggtgtca gtccgaactg tttttcgaag tagccgtaag
    29221 catcgtgaaa aacgaaataa cctttcccct tgagcggcgc gagctcgtta ccaacctgcg
    29281 tttcggttga ggctaattgt gcctcaaaat ccttcaggtt ggcgtcaagt ttggctcgac
    29341 tttgcggcat aagttccact aattttccat ggattgcaac cgctgtagcc cgcgctatct
    29401 ctggggaaag ccaaagatgc atgttgaaat cgccgtgatg gtgatcttcg tcactttttt
    29461 ccgcgtggtc gtgatcatca tcatcgtcgt gaatactttt catcagcagc ggtttcacat
    29521 cttctagctg cgcaatcgtt acctgtttcg cttctggtaa tttacttacc ggtttttgca
    29581 tgaacgcttc catctccggg ccaacccaaa cgactaagtc cgcgttctgt aagcgtttta
    29641 catccgatgg acgcagtgaa taatcatgtt ctgaagcgcc gtcaggcagt aaaacctgcg
    29701 tttctgttac cccatcagca atggcagaag cgatgaaccc aacgggttta agcgaagcga
    29761 caacggcggc atctgcggcc tgtgttgcac cgccccagag agcggcggat aatgctgcga
    29821 aaagaagcgt ttttttatgt aacataatgc gaccaatcat cgtaatgaat atgagaactg
    29881 tgatattata acatttcatg actactgcaa gactaaaatt aacatgacaa gtctggtttc
    29941 cctggaaaat gtctcggttt cttttggcca acgccgcgtc ctctctgatg tgtcgctgga
    30001 acttaaacct ggaaaaattt tgactttact tgggccaaac ggcgcaggta agtcgacact
    30061 ggtacgggta gtgctcgggc tggtaacacc cgatgaaggg gttatcaagc gcaacggaaa
    30121 actgcgcatc ggctatgtac cgcagaagct gtatctcgac accacgttgc cactgaccgt
    30181 aaaccgtttt ttacgcttac gccctggcac acataaagaa gatattttgc ctgcactgaa
    30241 acgtgtccag gccgggcatc tgattaacgc accgatgcaa aagctctcgg gtggcgaaac
    30301 gcagcgtgta ctgttagcgc gagcattgtt aaatcgaccg caattattag tgctggatga
    30361 acccactcag ggcgtggatg tgaatggtca ggtggcgtta tatgacctta ttgaccaact
    30421 gcgtcacgaa ctggattgtg gcgttttaat ggtatctcac gatctgcatc tggtaatggc
    30481 aaaaaccgat gaagtgcttt gcctgaatca ccacatttgt tgttccggca caccggaagt
    30541 tgtttccctg catccggagt ttatttctat gtttggtcct cgtggtgctg aacaactggg
    30601 tatctatcgc catcatcata atcatcgtca cgatttacag ggacgaattg ttttgcgtcg
    30661 gggaaatgat cgctcatgat tgaattatta tttcccggtt ggttagccgg gatcatgctc
    30721 gcctgtgccg cgggtccgct gggttcgttt gtagtctggc gtcgtatgtc ttatttcggt
    30781 gatacgctgg ctcatgcctc attacttggc gtcgcgtttg gtttgttgct ggacgtgaat
    30841 ccattctatg cggtgattgc agttacgctg ctgctggcgg gcggtctggt atggctggag
    30901 aagcgtccac agctggcgat cgacacgtta ttagggatta tggcgcacag tgccctgtcg
    30961 ctgggcctgg tggtcgttag tctgatgtct aatattcgtg ttgatttgat ggcttacctg
    31021 ttcggtgatt tactggcagt gacgccagaa gatctcatct ctattgcgat tggcgtggtc
    31081 atcgtggtgg ctattatgtt ctggcaatgg cgcaatttgc tgtcgatgac gattagcccg
    31141 gatctggcgt ttgttgatgg tgtgaaatta cagcgcgtga aattgttgtt gatgctggtg
    31201 acggcattga cgattggtgt agcgatgaaa tttgtcggcg cgttgattat tacttcactg
    31261 ctgattattc ctgctgctac tgcacgtcgc tttgcccgca cgccggaaca gatggctggt
    31321 gtcgctgttt tggtggggat ggtggcagtg actggcggtt taaccttttc cgcattttac
    31381 gatacacctg caggcccgtc ggtggtgcta tgtgcggcac tgttatttat tatcagtatg
    31441 atgaaaaagc aggccagcta atctatcgct gaacacattt gtcggatgcg gcgcgagcgc
    31501 cttatccgac ctatggttcg ctatctctgg tatatgcctg ataagacgcg aatagcggcg
    31561 catcaggcaa cactgccagt gtcggatgcg ccgcgagcga ccgatccgac ttacggcatt
    31621 tctggcggcg tgatgccgaa gtggttccac gcccgcactg tcgccatacg cccgcgcggt
    31681 gtacgctgca aaaagccttg ctgaatcaaa taaggttcca gcacatcctc aatggtttca
    31741 cgttcttcgc caatggcagc cgccaggtta tccagaccta ccggcccacc aaagaactta
    31801 tcgattaccg ccagcaacaa tttgcggtcc atataatcga aaccttcggc atcgacattc
    31861 agcatatcca gcgcctgagc agcgatatca gccgagatgg tgccatcgtg cttcacttcg
    31921 gcgaaatcac gcactcgacg cagcagacgg ttagcaatgc gcggcgtacc gcgagcgcga
    31981 cgagcaactt ccagcgcgcc gtcatcactc atctcaagcc ccataaagcg tgcgctgcga
    32041 ctgacgatat attgcagatc cggcacctga taaaactcca gacgctgcac aataccaaaa
    32101 cgatcgcgca acggtgatgt cagcgaacct gcgcgcgtgg ttgcgccaat cagtgtaaac
    32161 ggcggcaaat caattttaat ggagcgtgcc gccggacctt caccaatcat gatatccagt
    32221 tggtagtctt ccattgccgg atacaacacc tcttccacca ctggtgaaag acggtggatc
    32281 tcatcaataa acagtacatc gtgtggttca aggttagtga gcattgctgc cagatcgccc
    32341 gccttttcca gcaccggacc agaagtcgtg cgtaaattaa cgcccatttc attggcgaca
    32401 atattggcaa gcgtagtttt acccaacccc ggaggaccaa aaatcaacag atgatcgagg
    32461 gcatcgccgc gcagtttcgc tgctttgatg aaaatctcca tctgtgaacg aacctgcggt
    32521 tgaccaacat actcttccag taatttaggg cgaatggcgc gatctgccac atcttccggc
    32581 aaagtggtac cggcagaaat cagacggtct gcttcaatca tcctttacct cataacgcgg
    32641 cgcgtagggc ttcgcgaatt aatgtttcac tgctggcgtc agggcgagcg attttgctca
    32701 ccatacggct tgcttcttgt ggtttatagc ccagcgccac cagcgcagca accgcttcct
    32761 gttcagcatc gtcggtcgcc gggctggcag gtgacgtgag taccaggtcg gcggctggcg
    32821 taaagagatc gccatgcaaa cctttaaatc ggtctttcat ttcgacaatc aagcgttcgg
    32881 cggttttttt gccaataccc ggcagtttca ccagtgcccc cacttcttca cgctcaacgg
    32941 cattaacgaa ctgctgcgct gacattccgg agaggatcgc cagcgccaac ttcgggccga
    33001 cgccgttggt tttgatcaac tctttgaaca acgtgcgctc ttgtttattg ttaaaaccgt
    33061 acagcagttg cgcgtcttca cgcaccacaa agtgggtgaa aacgatcgct tcctgacccg
    33121 cttcagggag ttcataaaaa caggtcatcg gcatatgcac ttcatagcct acgccgccca
    33181 cttcaattaa caccagcggg ggttgttttt caatgatgat gcctctgagt ctgcctatca
    33241 catgacgctc ctgcgtaatg aatcaaagat aatgctgtat gataaaaaaa tgctggatag
    33301 atatccagcg aaggatgaag aaaacttgcg aggtgtcccg atgatctgaa
//
LOCUS       DAZFLD010000023        33131 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.023, whole genome shotgun sequence.
ACCESSION   DAZFLD010000023 DAZFLD010000000
VERSION     DAZFLD010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33131)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33131)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33131
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.023"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(90..455)
                     /gene="yhaH"
                     /locus_tag="ACHKBL_000980"
     CDS             complement(90..455)
                     /gene="yhaH"
                     /locus_tag="ACHKBL_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFO5817893.1"
                     /translation="MDWYLKVLKNYVGFRGRARRKEYWMFILVNIIFTFVLGLLDKML
                     GWQRAGGEGILTTIYGILVFLPWWAVQFRRLHDTDRSAWWALLFLIPFIGWLIIIVFN
                     CQAGTPGENRFGPDPKLEP"
     gene            complement(746..1732)
                     /gene="yqjG"
                     /locus_tag="ACHKBL_000981"
     CDS             complement(746..1732)
                     /gene="yqjG"
                     /locus_tag="ACHKBL_000981"
                     /EC_number="1.8.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionyl-hydroquinone reductase YqjG"
                     /protein_id="HFO5817894.1"
                     /translation="MGQLIDGVWHDTWYDTKSTGGKFQRSASAFRNWLTADGAPGPTG
                     TGGFIAEKDRYHLYVSLACPWAHRTLIMRKLKGLEPFISVSVVNPLMLENGWTFDDSF
                     PGATGDTLYQHEFLYQLYLHADPHYSGRVTVPVLWDKKNHTIVSNESAEIIRMFNTAF
                     DALGAKAGDYYPPALQTKIDELNGWIYDTVNNGVYKAGFATSQQAYDEAVAKVFESLA
                     RLEQILGQHRYLTGNQLTEADIRLWTTLVRFDPVYVTHFKCDKHRISDYLNLYGFLRD
                     IYQMPGIAETVNFNHIRNHYFRSHKTINPTGIISIGPWQDLDEPHGRDVRFG"
     gene            complement(1802..2194)
                     /gene="yqjF"
                     /locus_tag="ACHKBL_000982"
     CDS             complement(1802..2194)
                     /gene="yqjF"
                     /locus_tag="ACHKBL_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008503138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HFO5817895.1"
                     /translation="MKKLEDVGVLVARILMPILFITAGWGKITGYAGTQQYMEAMGVP
                     GFMLPLVILLEFGGGLAILFGFLTRTTALFTAGFTLLTAFLFHSNFAEGVNSLMFMKN
                     LTISGGFLLLAITGPGAYSIDRLLNKKW"
     gene            complement(2381..2680)
                     /gene="yqjK"
                     /locus_tag="ACHKBL_000983"
     CDS             complement(2381..2680)
                     /gene="yqjK"
                     /locus_tag="ACHKBL_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqjK-like family protein"
                     /protein_id="HFO5817896.1"
                     /translation="MSSKVERERRKAQLLSQIQQQRLDLSASRREWLEATGAYDRRWN
                     MLLSLRSWALVGSSVMAIWTIRHPNMLVRWARRGFGVWSAWRLVKTTLKQQQLRG"
     gene            complement(2670..3074)
                     /gene="yqjE"
                     /locus_tag="ACHKBL_000984"
     CDS             complement(2670..3074)
                     /gene="yqjE"
                     /locus_tag="ACHKBL_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFO5817897.1"
                     /translation="MADTHHAQGPGKSVLGIGQRIVSIMVEMVETRLRLAVVELEEEK
                     ANLFQLLLMLGLTMLFAAFGLMSLMVLIIWAVDPQYRLNAMIATTVVLLLLALIGGIW
                     TLRKSRKSTLLRHTRHELANDRQLLEEESREQ"
     gene            complement(3077..3382)
                     /gene="yqjD"
                     /locus_tag="ACHKBL_000985"
     CDS             complement(3077..3382)
                     /gene="yqjD"
                     /locus_tag="ACHKBL_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqjD family protein"
                     /protein_id="HFO5817898.1"
                     /translation="MSKEHTTEHLRAELKSLSDTLEEVLSSSGEKSKEELSKIRSKAE
                     QALKQSRYRLGETGDAIAKQTRVAAARADEYVRENPWTGVGIGAAIGVVLGVLLSRR"
     gene            complement(3420..3788)
                     /gene="yqjC"
                     /locus_tag="ACHKBL_000986"
     CDS             complement(3420..3788)
                     /gene="yqjC"
                     /locus_tag="ACHKBL_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1090 family protein YqjC"
                     /protein_id="HFO5817899.1"
                     /translation="MKYRIALAVSLFALSAGSYATTLCQEKEQNILKEISYAEKHQNQ
                     NRIDGLNKALSEVRANCSDSQLRADHQKKIAKQKDEVAERQQDLAEAKQKGDADKIAK
                     RERKLAEAQEELKKLEARDY"
     gene            complement(3935..4318)
                     /gene="mzrA"
                     /locus_tag="ACHKBL_000987"
     CDS             complement(3935..4318)
                     /gene="mzrA"
                     /locus_tag="ACHKBL_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EnvZ/OmpR regulon moderator MzrA"
                     /protein_id="HFO5817900.1"
                     /translation="MQIPRMSLRQLAWSGAVLLLVGTLLLAWSAVRQQESTLAIRAVH
                     QGTTMPDGFSIWHHLDAHGIPFKSITPKNDTLLITFDSSDQSAAAKAVLDRTLPHGYI
                     IAQQDNNSQAMQWLTRLRDNSHRFG"
     gene            complement(4322..4984)
                     /gene="yqjA"
                     /locus_tag="ACHKBL_000988"
     CDS             complement(4322..4984)
                     /gene="yqjA"
                     /locus_tag="ACHKBL_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family general envelope maintenance protein
                     YqjA"
                     /protein_id="HFO5817901.1"
                     /translation="MELLTQLLQALWAQDFETLANPSMIGMLYFVLFVILFLENGLLP
                     AAFLPGDSLLVLVGVLIAKGAMGYPQTILLLTVAASLGCWVSYIQGRWLGNTRTVQNW
                     LSHLPAHYHQRAHHLFHKHGLSALLIGRFIAFVRTLLPTIAGLSGLNNARFQFFNWMS
                     GLLWVLILTTLGYMLGKTPVFLKYEDQLMSCLMLLPVVLLVFGLAGSLVVLWKKKYGN
                     RG"
     gene            complement(5006..5089)
                     /locus_tag="ACHKBL_000989"
     CDS             complement(5006..5089)
                     /locus_tag="ACHKBL_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5817902.1"
                     /translation="MLMYQTRRTYQDSNNIAVVHHLKPAWR"
     gene            complement(5329..6105)
                     /gene="exuR"
                     /locus_tag="ACHKBL_000990"
     CDS             complement(5329..6105)
                     /gene="exuR"
                     /locus_tag="ACHKBL_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ExuR"
                     /protein_id="HFO5817903.1"
                     /translation="MEITEPRRLYQQLAADLKERIEQGVYLVGDKLPAERFIADEKNV
                     SRTVVREAIIMLEVEGYVEVRKGSGIHVVSNQPRHQQATDNNMEFANYGPFELLQARQ
                     LIESNIAEFAATQVTKQDIMKLMAIQEQARGEQCFRDSEWDLQFHIQVALATQNSALA
                     AIVEKMWTQRSHNPYWKKLHEHIDARTVDNWCDDHDQILKALIRKDPHAAKLAMWQHL
                     ENTKIMLFNETSDDFEFNADRYLFAENPVVHLDTATSGSK"
     gene            complement(6235..7533)
                     /gene="exuT"
                     /locus_tag="ACHKBL_000991"
     CDS             complement(6235..7533)
                     /gene="exuT"
                     /locus_tag="ACHKBL_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexuronate transporter ExuT"
                     /protein_id="HFO5817904.1"
                     /translation="MRKIKGLRWYMIALVTLGTVLGYLTRNTVAAAAPTLMEELNIST
                     QQYSYIIAAYSAAYTVMQPVAGYVLDVLGTKIGYAMFAVLWAVFCGATALAGSWGGLA
                     VARGAVGAAEAAMIPAGLKASSEWFPAKERSIAVGYFNVGSSIGAMIAPPLVVWAIVM
                     HSWQMAFIISGALSFIWAMAWLIFYKHPRDQKHLTDEERDYIINGQEAQHQVDTAKKM
                     SVGQILRNRQFWGIALPRFLAEPAWGTFNAWIPLFMFKVYGFNLKEIAMFAWMPMLFA
                     DLGCILGGYLPPLFQRWFGVNLIVSRKMVVTLGAVLMIGPGMIGLFTNPYVAIMLLCI
                     GGFAHQALSGALITLSSDVFGRNEVATANGLTGMSAWLASTLFALVVGALADTIGFSP
                     LFAVLAVFDLLGALVIWTVLQNKPAIEVAQESHNDPAPQH"
     gene            8016..9428
                     /gene="uxaC"
                     /locus_tag="ACHKBL_000992"
     CDS             8016..9428
                     /gene="uxaC"
                     /locus_tag="ACHKBL_000992"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFO5817905.1"
                     /translation="MTPFMTEDFLLDTEFARRLYHDYAKDQPIFDYHCHLPPQQIAED
                     YRFKNLYDIWLKGDHYKWRAMRTNGVAERLCTGDASDREKFDAWAATVPHTIGNPLYH
                     WTHLELRRPFGITGKLLSPSTADEIWNECNELLAQDNFSARGIMQQMNVKMVGTTDDP
                     IDSLEHHAEIAKDGSFTIKVLPSWRPDKAFNIEQATFNDYMAKLGEVSDTDIRRFADL
                     QTALTKRLDHFAAHGCKVSDHALDVVMFAEANEAELDSILARRLAGETLSEHEVAQFK
                     TAVLVFLGAEYARRGWVQQYHIGALRNNNLRQFKLLGPDVGFDSINDRPMAEELSKLL
                     SKQNEENLLPKTILYCLNPRDNEVLGTMIGNFQGEGMPGKMQFGSGWWFNDQKDGMER
                     QMTQLAQLGLLSRFVGMLTDSRSFLSYTRHEYFRRILCQMIGRWVEAGEAPADINLLG
                     EMVKNICFNNARDYFAIELN"
     gene            9443..10930
                     /gene="uxaA"
                     /locus_tag="ACHKBL_000993"
     CDS             9443..10930
                     /gene="uxaA"
                     /locus_tag="ACHKBL_000993"
                     /EC_number="4.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HFO5817906.1"
                     /translation="MQYIKIHALDNVAVALADLAEGTEVSVDNQTVTLRQDVARGHKF
                     ALTDIAKGANVIKYGLPIGYALADIAAGEHVHAHNTRTNLSDLDQYRYQPDFQDLPAQ
                     AADREVQIYRRANGDVGVRNELWILPTVGCVNGIARQIQNRFLKETNNAEGTDGVFLF
                     SHTYGCSQLGDDHINTRTMLQNMVRHPNAGAVLVIGLGCENNQVAAFRETLGDIDPER
                     VHFMICQQQDDEIEAGIAHLHQLYNVMRNDKREPGKLSELKFGLECGGSDGLSGITAN
                     PMLGRFSDYVIANGGTTVLTEVPEMFGAEQLLMDHCRDEATFEKLVTMVNDFKQYFIA
                     HDQPIYENPSPGNKAGGITTLEDKSLGCTQKAGSSVVVDVLRYGERLKTPGLNLLSAP
                     GNDAVATSALAGAGCHMVLFSTGRGTPYGGFVPTVKIATNSELAAKKKHWIDFDAGQL
                     IHGKAMPQLLEEFIDTIVEFANGKQTCNERNDFRELAIFKSGVTL"
     gene            11013..11564
                     /gene="ygjV"
                     /locus_tag="ACHKBL_000994"
     CDS             11013..11564
                     /gene="ygjV"
                     /locus_tag="ACHKBL_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HFO5817907.1"
                     /translation="MTAYWLAQGVGVIAFLIGITTFFNRDERRFKKQLSVYSAVIGVH
                     FFLLGTYPAGASAILNAIRTLITLRTRSLWVMAIFIVLTGGIGLAKFHHPVELLPVIG
                     TIVSTWALFRCKGLTMRCVMWFSTCCWVIHNFWAGSIGGTMIEGSFLLMNGLNIIRFW
                     RMQKRGIDPFKVEKTPPAIDERG"
     gene            complement(11569..12813)
                     /gene="sstT"
                     /locus_tag="ACHKBL_000995"
     CDS             complement(11569..12813)
                     /gene="sstT"
                     /locus_tag="ACHKBL_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HFO5817908.1"
                     /translation="MTTQRSPGLFRRLAHGSLVKQILVGLVLGILLAWISKPAAEAVG
                     LLGTLFVGALKAVAPILVLMLVMASIANHQHGQKTNIRPILFLYLLGTFSAALAAVIF
                     SFAFPSTLHLSSSAGDISPPSGIVEVMRGLVMSMVSNPIDALLKGNYIGILVWAIGLG
                     FALRHGNETTKNLINDMSNAVTFMVKLVIRFAPIGIFGLVSSTLATTGFSTLWGYAQL
                     LVVLVGCMLLVALVVNPLLVWWKIRRNPFPLVLLCLRESGVYAFFTRSSAANIPVNMA
                     LCEKLNLDRDTYSVSIPLGATINMAGAAITITVLTLAAVNTLGIPVDLPTALLLSVVA
                     SLCACGASGVAGGSLLLIPLACNMFGISNDIAMQVVAVGFIIGVLQDSCETALNSSTD
                     VLFTAAACQAEDDRLANSALRN"
     gene            complement(13429..14394)
                     /gene="alx"
                     /locus_tag="ACHKBL_000996"
     CDS             complement(13429..14394)
                     /gene="alx"
                     /locus_tag="ACHKBL_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family membrane protein Alx"
                     /protein_id="HFO5817909.1"
                     /translation="MNTVGTPLLWGGFAVVVTIMLAIDLLLQGRRGAHAMTMKQAAAW
                     SLVWVTLSLLFNAAFWWYLVQTEGRAVADPQALAFLTGYLIEKSLAVDNVFVWLMLFS
                     YFSVPAALQRRVLVYGVLGAIVLRTIMIFTGSWLISQFDWILYIFGAFLLFTGVKMAL
                     AHEDESGIGDKPLVRWLRGHLRMTDTIDNEHFFVRKNGLLYATPLMLVLILVELSDVI
                     FAVDSIPAIFAVTTDPFIVLTSNLFAILGLRAMYFLLAGVAERFSMLKYGLAVILVFI
                     GIKMLIVDFYHIPIAVSLGVVFGILVMTFIINAWVNYRHDKQRGE"
     gene            complement(14677..15663)
                     /gene="ygjR"
                     /locus_tag="ACHKBL_000997"
     CDS             complement(14677..15663)
                     /gene="ygjR"
                     /locus_tag="ACHKBL_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311996.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFO5817910.1"
                     /translation="MIRFAVIGTNWITRQFVEAAHESGKYKLTAVYSRSLEQAQHFAN
                     DFSVEHLFTSLEAMAESDAIDAVYIASPNALHFSQTQLFLSHKIHVICEKPLASNLAE
                     VDAAIACARENQVVLFEAFKTAYLPNFHLLRQALPKVGKLRKVFFNYCQYSSRYQRYL
                     DGENPNTFNPAFSNGSIMDIGFYCLASAVALFGEPKNVQATASLLASGVDAHGVVVMD
                     YGDFSVTLQHSKVSDSVLASEIQGEAGSLVIEKLSECQKVCFVPRGSQMQDLTQPQHI
                     NTMLYEAELFAELVDEHLVDHPGLAVSRITAKLLTEIRRQTGVIFPADSVKL"
     gene            complement(15742..16425)
                     /gene="ygjQ"
                     /locus_tag="ACHKBL_000998"
     CDS             complement(15742..16425)
                     /gene="ygjQ"
                     /locus_tag="ACHKBL_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin high temperature exclusion protein"
                     /protein_id="HFO5817911.1"
                     /translation="MQARLLLRICFSRRTLKIGCLLLLIAGATIFIADRVMVNTSKQL
                     TWSDVSAVPARNVGLLLGARPGNRYFTRRIDTAAALYHAGKVKWLLVSGDNGRKNYDE
                     ASGMQQALIAKGVPAKVIFCDYAGFSTLDSVVRAKKVFGENHITIISQEFHNQRAIWL
                     AKQYGIDAIGFNAPDLNMKHGFYTQLREKLARVGAVIDAKILHRQPKYLGPSVMIGPF
                     SEHGCPAQK"
     gene            complement(16502..17005)
                     /gene="ygjP"
                     /locus_tag="ACHKBL_000999"
     CDS             complement(16502..17005)
                     /gene="ygjP"
                     /locus_tag="ACHKBL_000999"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HFO5817912.1"
                     /translation="MSNLTYLQGYPEQLLSQVRTLINEQRLGDVLAKRYPGTHDYATD
                     KALWQYTQDLKNQFLRNAPPITKVMYDNKIHVLKNALGLHTAVSRVQGGKLKAKAEIR
                     VATVFRNAPEPFLRMIVVHELAHLKEKEHNKAFYQLCCHMEPQYHQLEFDTRLWLTQL
                     SLGQDKI"
     gene            17090..18226
                     /gene="rlmG"
                     /locus_tag="ACHKBL_001000"
     CDS             17090..18226
                     /gene="rlmG"
                     /locus_tag="ACHKBL_001000"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(1835)-N(2))-methyltransferase
                     RlmG"
                     /protein_id="HFO5817913.1"
                     /translation="MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEI
                     RGPVLILNDAFGALSCALAEHKPYSIGDSYISELATRENLRLNGIDESSVKFLDSTAD
                     YPQQPGVVLIKVPKTLALLEQQLRALRKVVTPDTRIIAGAKARDIHTSTLELFEKVLG
                     PTTTTLAWKKARLINCTFNEPPLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGAR
                     FFMQHLPENLEGEIVDLGCGNGVIGLTLLDKNPQAKVVFVDESPMAVASSRLNVETNM
                     PEALDRCEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAWEMFHHARRCLKING
                     ELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKAVKLGRRR"
     gene            18417..18500
                     /locus_tag="ACHKBL_001001"
                     /pseudo
     CDS             18417..18500
                     /locus_tag="ACHKBL_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311992.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
     gene            18497..18913
                     /gene="higA"
                     /locus_tag="ACHKBL_001002"
     CDS             18497..18913
                     /gene="higA"
                     /locus_tag="ACHKBL_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HigA"
                     /protein_id="HFO5817914.1"
                     /translation="MIAIADILQAGEKLTAVAPFLAGIQNEEQYAQALELVDHLLLND
                     PENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGGIAVIRTLMDQYGLTLSDLPEIG
                     SKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID"
     gene            complement(18958..20976)
                     /gene="fadH"
                     /locus_tag="ACHKBL_001003"
     CDS             complement(18958..20976)
                     /gene="fadH"
                     /locus_tag="ACHKBL_001003"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HFO5817915.1"
                     /translation="MSYPSLFAPLDLGFTTLKNRVLMGSMHTGLEEYPDGAERLAAFY
                     AERARHGVALIVSGGIAPDLTGVGMEGGAMLNDASQIPHHRTITEAVHQEGGKIALQI
                     LHTGRYSYQPHLVAPSALQAPINRFVPHELSHEEILQLIDNFARCAQLAREAGYDGVE
                     VMGSEGYLINEFLTLRTNQRSDQWGGDYRNRMRFAVEVVRAVRERVGNDFIIIYRLSM
                     LDLVEGGGTFAETVGLAQAIEAAGATIINTGIGWHEARIPTIATPVPRGAFSWVTRKL
                     KSHVSLPLVTTNRINDPQVADDILSRGDADMVSMARPFLADAELLSKAQSGRADEINT
                     CIGCNQACLDQIFVGKVTSCLVNPRACHETKMPILPAVQKKNLAVVGAGPAGLAFAIN
                     AAARGHQVTLFDAHSEIGGQFNIAKQIPGKEEFYETLRYYRRMIEVTGVTLKLNHTVT
                     ADQLQSFDETILASGIVPRIPPIDGIDHPKVLSYLDVLRDKAPVGKKVAIIGCGGIGF
                     DTAMFLSQPGESTSQNIAEFCNEWGIDSSLQQAGGLSPQGMQIPRSPRQIVMLQRKAS
                     KPGQGLGKTTGWIHRTTLLSRGVKMIPGVSYQKIDDDGLHVVINGETQVLAVDNVVIC
                     AGQEPNRALAQQLIDSGKTVHLIGGCDVAMELDARRAIAQGTRLALEI"
     gene            complement(21147..23498)
                     /gene="ygjK"
                     /locus_tag="ACHKBL_001004"
     CDS             complement(21147..23498)
                     /gene="ygjK"
                     /locus_tag="ACHKBL_001004"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFO5817916.1"
                     /translation="MKIKTILTPVTCALLISFSAHAANADNYKNVINRTGAPQYMKDY
                     DYDDHQRFNPFFDLGAWHGHLLPDGPNTMGGFPGVALLTEEYINFMASNFDRLTVWQD
                     GKKVDFTLEAYSIPGALVQKLTAKDVQVEMTLRFATPRTSLLETKIISNKPLDLVWDG
                     ELLEKLEAKEGKPLSDKTIAGEYPDYQRKISATRDGLKVTFGKVRATWDLLTSGESEY
                     QVHKSLPVQTEINGNRFTSKAHINGSATLYTTYSHLLTAQEVSKEQMQIRDILARPAF
                     YLTASQQRWEEYLKKGLTNPDATPEQTRVAVKAIETLNGNWRSPGGAVKYNTVTPSVT
                     GRWFSGNQTWPWDTWKQAFAMAHFNPDIAKENIRAVFSWQIQPGDRVRPQDVGFVPDL
                     IAWNLSPERGGDGGNWNKRNTKPSLAAWSVMEVYNVTQDKTWLAEMYPKLVAYHDWWL
                     RNRDHNGNGVPEYGATRDKAHNTESGEMLFTVKKGNKAETLSGLNNYARVVEKGQYDS
                     LEIPAQVAASWESGRDDAAVFGFIDKEQLDKYVANGGKRSDWTVKFAENRSQDGTLLG
                     YSLLQESVDQASYMYSDNHYLAEMATILGKPEEAKRYRQLAQQLADYINTCMFDPTTQ
                     FYYDVRIEDKPLANGCAGKPIVERGKGPEGWSPLFNGAATQANADAVVKVMLDPKEFN
                     TFVPLGTAALTNPAFGADIYWRGRVWVDQFWFGLKGMERYGYRDDALKLADTFFRHAK
                     GLTADGPIQENYNPLTGAQQGAPNFSWSAAHLYMLYNDFFRKQ"
     gene            complement(23515..24585)
                     /gene="ygjJ"
                     /locus_tag="ACHKBL_001005"
     CDS             complement(23515..24585)
                     /gene="ygjJ"
                     /locus_tag="ACHKBL_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgjJ"
                     /protein_id="HFO5817917.1"
                     /translation="MKLITAPCRALLALPFCYAFSVTADEARPAEHDDTKTPAMTSTS
                     SPSFRFYGELGVGGYMDLEGENKHKYSDGTYIEGGLEMKYGSWFGLIYGEGWTVQADH
                     DGNAWVPDHSWGGFEGGINRFYGGYRTNDGTEIMLSLRQDSSLDDLQWWGDFTPDLGY
                     VIPNTRDIMTALKVQNLSGNFRYSVTATPAGHHDESKAWLHFGKYDRYDDKYTYPAMM
                     NGYIQYDLAEGITWMNGLEITDGTGQLYLTGLLTPNFAARAWHHTGRADGLDVSGSES
                     GMMVSAMYEALKGIYLSTAYTYAKHRPDHADDETTSFMQFGIWYEYGGGRFATAFDSR
                     FYMKNASHDPSDQLFLMQYFYW"
     gene            complement(24719..26152)
                     /gene="ygjI"
                     /locus_tag="ACHKBL_001006"
     CDS             complement(24719..26152)
                     /gene="ygjI"
                     /locus_tag="ACHKBL_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFO5817918.1"
                     /translation="MSDTKRNTIGKFGLLSLTFAAVYSFNNVINNNIELGLASAPMFF
                     LATIFYFIPFCLIIAEFVSLNKNSEAGVYAWVKSSLGGRWAFITAYTYWFVNLFFFTS
                     LLPRVIAYASYAFLGYEYIMTPVATTIISMVLFAFSTWVSTNGAKMLGPITSVTSTLM
                     LLLTLSYILLAGTALVGGVQPADPITVDAMIPNFNWAFLGVTTWIFMAAGGAESVAVY
                     VNDVKGGSKSFVKVIILAGIFIGVLYSVSSVLINVFVSSKELKFTGGSVQVFHGMAAY
                     FGLPEALMNRFVGLVSFTAMFGSLLMWTATPVKIFFSEIPEGIFGKKTVELNENGVPA
                     RAAWIQFLIVIPLMIIPMLGSNTVQDLMNTIINMTAAASMLPPLFIMLAYLNLRAKLD
                     HLPRDFRMGSRRTGIIVVSMLIAIFAVGFVASTFPTGANILTIIFYNVGGIVIFLGFA
                     WWKYSKYIKGLTAEERHIEATPASNVD"
     gene            complement(26215..26664)
                     /gene="ebgC"
                     /locus_tag="ACHKBL_001007"
     CDS             complement(26215..26664)
                     /gene="ebgC"
                     /locus_tag="ACHKBL_001007"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit beta"
                     /protein_id="HFO5817919.1"
                     /translation="MRIIDNLEQFRQIYASGKKWQRCVEAIENIDNIQPGVAHSIGDS
                     LTYRVETDSATDALFTGHRRYFEVHYYLQGQQKIEYAPKETLQVVEYYRDETDREYLK
                     GCGETVEVHEGQIVIYDIHEAYRFICNNAVKKVVLKVTIEDGYFHNK"
     gene            complement(26661..29753)
                     /gene="ebgA"
                     /locus_tag="ACHKBL_001008"
     CDS             complement(26661..29753)
                     /gene="ebgA"
                     /locus_tag="ACHKBL_001008"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit alpha"
                     /protein_id="HFO5817920.1"
                     /translation="MNRWENIQLTHENRLAPRAYFFSYDSVAQARTFARETSSLFLPL
                     SGQWNFHFFDHPLQVPEAFTSELMADWGHITVPAMWQMEGHGKLQYTDEGFPFPIDVP
                     FVPSDNPTGAYQRIFTLSDGWQGKQTLIKFDGVETYFEVYVNGQYVGFSKGSRLTAEF
                     DISAMVKTGDNLLCVRVMQWADSTYVEDQDMWWSAGIFRDVYLIGKQLTHINDFTVRT
                     DFDEAYCDATLSCEVVLENLAASPVVTTLEYTLFDGEHVVHSSTIDHLAIEKLTSASF
                     AFTVEQPQQWSAESPYLYHLVMTLKDADGNVLEVVPQRVGFRDIKVRDGLFWINNRYV
                     MLHGVNRHDNDHRKGRAVGMDRVEKDLQLMKQHNINSVRTAHYPNDPRFYELCDIYGL
                     FVMAETDVESHGFANVGDISRITDDPQWEKVYVERIVRHIHAQKNHPSIIIWSLGNES
                     GYGCNIRAMYHAAKALDDTRLVHYEEDRDAEVVDIISTMYTRVPLMNEFGEYPHPKPR
                     IICEYAHAMGNGPGGLTEYQNVFYKHDCIQGHYVWEWCDHGIQAQDDNGNVWYKFGGD
                     YGDYPNNYNFCLDGLIYSDQTPGPGLKEYKQVIAPVKIHALDLTRGELKVENNLWFTT
                     LDDYTLHAEVRAEGETLATQQIKLRDVAPNSEAPLQITLPQLDAREAFLNITVTKDSR
                     TRYSEAGHSIATYQFPLKENTAQPVPFAPNNARPLTLEDDRLSCTVRGYNFAITFSKT
                     SGKPTSWQVNGESLLTREPKINFFKPMIDNHKQEYEGLWQPNHLQIMQEHLRDFAVEQ
                     SDGEVLIISRTVIAPPVFDFGMRCTYIWRIAADGQVNVALSGERYGDYPHIIPCIGFT
                     MGINGEYDQVAYYGRGPGENYADSQQANIIDIWRSTVDAMFENYPFPQNNGNRQHVRW
                     TALTNRHGNGLLVVPQRPINFSAWRYTQENIHAAQHCNELQRSDDITLNLDHQLLGLG
                     SNSWGSEVLDSWRVWFRDFSYGFTLLPVSGGEATAQSLASYEFGAGFFSTNLHSENKQ
                     "
     gene            complement(29937..30920)
                     /gene="ebgR"
                     /locus_tag="ACHKBL_001009"
     CDS             complement(29937..30920)
                     /gene="ebgR"
                     /locus_tag="ACHKBL_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator EbgR"
                     /protein_id="HFO5817921.1"
                     /translation="MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEK
                     LEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLAIELTN
                     CYEHNGLPDIKNVTGILIVGKPTSALRAAACALTDNICFIDFHEPGSGYDAVDIDLAR
                     ISKEIIDFYINQGVNRIGFIGGEDLPGKADIREVAFAEYGRLKHVVREEDIWRGGFSS
                     SSGYELAKQMLAREDYPKALFVASDSIAIGVLRAIHERGLNIPQDISLISVNDIPTAR
                     FTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKLKLRGTTR"
     gene            31139..31471
                     /gene="ygjH"
                     /locus_tag="ACHKBL_001010"
     CDS             31139..31471
                     /gene="ygjH"
                     /locus_tag="ACHKBL_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HFO5817922.1"
                     /translation="METVAYADFARLEMRVGKIVEVKRHENADKLYIVQVDVGEKTLQ
                     TVTSLVPYYSEEELMGKTVVVLCNLQKAKMRGETSECMLLCAETDDGSESVLLTPERM
                     MPAGVRIV"
     gene            complement(31513..32892)
                     /gene="ygjG"
                     /locus_tag="ACHKBL_001011"
     CDS             complement(31513..32892)
                     /gene="ygjG"
                     /locus_tag="ACHKBL_001011"
                     /EC_number="2.6.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417544.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0033094 - putrescine--2-oxoglutarate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009447 - putrescine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine aminotransferase"
                     /protein_id="HFO5817923.1"
                     /translation="MNRLPSSASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKE
                     HVNPGFLEYRKSVTAGGDYGAVEWQAGGLNTLVDTQGQEFIDCLGGFGIFNVGHRNPV
                     VVSAVQNQLAKQPLHSQELLDPLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKL
                     AKAYQSPRGKFTFIATSGAFHGKSLGALSATAKSTFRKPFMPLLPGFRHVPFGNIEAM
                     RTALNECKKTGDDVAAVILEPIQGEGGVILPPPGYLTAVRKLCDEFGALMILDEVQTG
                     MGRTGKMFACEHENVQPDILCLAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFG
                     GNPLACAAALATINVLLEQNLPAQAEQKGDMLLDGFRQLAREYPDLVQEARGKGMLMA
                     IEFVDNEIGYNFASEMFRQRVLVAGTLNNAKTIRIEPPLTLTIEQCEQVIKAARKALA
                     AMRVSVEEA"
BASE COUNT         8078 a         8716 c         8558 g         7779 t
ORIGIN      
        1 aatattttaa ataaacatta gtaaattatt cattcatcaa cataatgata aaagccggat
       61 gagtcagaca tccggcgagt agatttgttt taaggttcca gttttggatc aggtccaaag
      121 cggttttcgc ctggcgtacc cgcctggcag ttaaagacga tgataataag ccagccgata
      181 aacgggatta agaacagcag tgcccaccac gccgagcggt cggtgtcgtg caggcggcgg
      241 aactgaactg cccaccacgg taaaaacacc agaataccgt agattgtcgt caggatgcct
      301 tcgccaccgg cacgttgcca gcctaacatt ttatccagca gccccagcac gaacgtaaag
      361 atgatgttga ccagaataaa catccagtac tctttgcgcc gcgcacgacc ccggaaaccg
      421 acataatttt ttagtacttt cagataccag tccatttttg cctcaatttt gctcaatcac
      481 ttgtttttaa agcagcaaat taagaataga cgtgaatgaa atggcagtaa atattcgttc
      541 aaggaattaa taaagtgccg taaagcgcgc taagactgat gacaagcgca aaatcgccag
      601 atgcgctacg cttatctggc ctacgcggca ttgtaacata ttgaatataa acgttttgta
      661 ggttggagaa gacgttcacg ccgcatccga cataaataac acgcacttag ctaataatca
      721 gaggcacctc gtaaggtgcc tttttttaac cgaagcgaac atctcgtcca tgcggttcat
      781 cgagatcctg ccacggacca attgaaataa tccccgtagg gttgatggtc ttatggctgc
      841 ggaagtaatg attacggatg tgattgaaat tgacagtttc ggcgatcccc ggcatctggt
      901 agatatcgcg cagaaaaccg tacagattca ggtaatcgct gatgcggtgc ttatcacact
      961 tgaagtgggt cacatacacc ggatcaaaac ggaccagcgt ggtccacagg cgaatatcgg
     1021 cttcggttag ctgattgccg gtcaggtaac gatgctggcc taaaatctgc tccagtcgcg
     1081 ccagcgattc aaacactttc gccaccgcct cgtcgtaagc ttgctggctg gtggcaaaac
     1141 cagctttata cacgccgttg ttaacggtgt cataaatcca gccgttaagt tcgtcaattt
     1201 tcgtttgcag ggctggtggg tagtaatcac ccgctttcgc gcccagcgca tcaaacgcgg
     1261 tattgaacat gcggatgatt tccgctgatt cgttgctgac gatggtgtgg ttctttttgt
     1321 cccacagtac gggaacagta actcgtccgc tgtagtgtgg atcggcgtgg agataaagct
     1381 gatacaaaaa ttcatgttga tagagtgtat cgccggttgc acccggaaaa ctgtcatcaa
     1441 aggtccagcc gttttccagc atcagcgggt tcactacaga aacggaaata aacggttcca
     1501 gtcctttgag tttgcgcatg atcagcgtgc ggtgcgccca cgggcaggcg agtgaaacat
     1561 agagatgata acgatctttc tctgcgataa aaccgcctgt gccagtgggg cctggcgcgc
     1621 catcggcagt gagccagtta cgaaatgcgg aagctgaacg ttgaaattta ccgccggtag
     1681 atttggtatc gtaccaggtg tcatgccaga cgccgtcaat cagttgaccc atatttctct
     1741 cctccaatag caaaaacgag gagcttcctc ctcgtttttt atcgttaagt atagcgcgtg
     1801 cttaccactt tttattcagc aggcggtcga tgctatacgc gcccggaccg gtaattgcca
     1861 gcagcaggaa tccgccagaa attgtcaggt ttttcatgaa catcagcgag ttgacgcctt
     1921 cagcaaagtt gctgtgaaat aaaaatgccg tcagcagcgt aaagcccgca gtaaacaggg
     1981 ctgtggtgcg agtcaggaaa ccgaacagga ttgccagacc accaccaaac tcaagcagaa
     2041 tcaccagcgg cagcataaaa cccgggacgc ccatcgcttc catatattgt tgggtacccg
     2101 cgtaaccagt aatttttccc cagcctgcgg taataaacag aatcggcatt aaaatgcgcg
     2161 ctaccagtac accaacatct tctaattttt tcatcatact ctccactgcg ccgctcaggc
     2221 tgctgattgt ttttttgtgc aagggcgcgc tattagcgtc gttgctgtcg atggagagaa
     2281 tcataaacgt ggcgaagcgt gattgttagc aaggaaaact gtcaaatatt ttcaaaaaat
     2341 ttgagggata aggccggaat ttctccggcc agagaggaag ttaaccgcga agctgttgtt
     2401 gcttgagggt tgttttcacc agacgccagg cgctccatac accaaaaccg cgtctggccc
     2461 agcggaccag catattagga tggcgaatcg tccagatagc catcacgcta ctgccaacca
     2521 gcgcccagga gcgcagactc aacagcatat tccagcgacg atcgtaagcg cctgttgcct
     2581 ccagccattc acgacgactg gcggaaagat ccagccgttg ttgctggatc tggctaagca
     2641 gttgcgcctt acgtcgttca cgttcgactt tactgctcac gggactcctc ctcgagcagc
     2701 tgccgatcgt ttgccagctc atggcgcgtg tggcgcagca acgtagactt acgcgattta
     2761 cgtaacgtcc agatgccgcc aatcagtgcc agtagcagca acaccacggt ggtggcaatc
     2821 atcgcattca ggcgatattg cggatcaacc gcccagataa ttagcaccat caggctcata
     2881 agaccaaatg cagcgaaaag catcgtcagg cccagcatca gtaaaagttg aaagagattc
     2941 gctttttcct cttccagctc caccaccgcc agccgcagac gtgtctccac catttcaacc
     3001 atgatggaaa caattcgctg cccgatgccc agaacgcttt taccgggccc ttgcgcgtga
     3061 tgagtgtccg ccataattaa cgacgcgaca gcagaacacc gagcactaca ccgattgctg
     3121 cgccaatgcc cacgcccgtc cacggatttt cacgcacata ctcatcggca cgcgccgccg
     3181 cgacacgggt ttgtttggca atggcatcac cggtttcacc caggcgataa cggctctgtt
     3241 tcagtgcctg ctccgctttg ctacgaatct tactcaactc ttctttcgac ttctcgccag
     3301 atgagctaag cacctcttcc agcgtatcgg aaagggattt caactcagca cgcagatgtt
     3361 ccgtagtgtg ttctttcgac atagttttct ccaggtgagt aagtagtgac tattgtgagt
     3421 tagtagtcgc gcgcttccag ctttttcagc tcttcctgcg cttctgccag tttccgttcg
     3481 cgtttggcaa tcttatctgc atcgcctttt tgcttcgcct cggctaaatc ttgctggcgt
     3541 tccgccacct catctttctg ctttgcgatt ttcttctgat gatcggcacg cagctggcta
     3601 tctgaacagt tggcccggac ttcactcagg gctttattca gaccgtcaat acgattctgg
     3661 ttttggtgtt tttcggcata gctgatctcc ttaaggatat tttgctcctt ttcctgacac
     3721 agggtagtgg cataactacc ggcactaaga gcaaagagag aaacagctaa agcgatgcgg
     3781 tatttcattc ttgaaccttc cattgtgatt cggacctatt acaaaggaac gattgtccag
     3841 acaacgaagc ggtcgtgtta accaccgcct cattaatcat agacagttac ggtgaaatca
     3901 ccaaagtgag tgatctgttt cggataacag gaagttatcc gaagcgatga gagttatccc
     3961 gcaaccgggt cagccactgc atagcctgac tgttattgtc ctgctgcgca atgatgtagc
     4021 catggggcaa tgttctgtcg aggaccgctt ttgcggcggc gctctggtcg ctggagtcaa
     4081 aggtaattaa cagagtgtcg tttttggggg tgatactttt gaaaggaatg ccgtgagcgt
     4141 caaggtgatg ccagattgaa aaaccgtctg gcatcgttgt cccttgatga acggcacgaa
     4201 tcgccagcgt agactcttgc tggcgaaccg cggaccaggc cagcaacagt gtgccgacca
     4261 gaagtaaaac agcgccggac caggctagct ggcgaagcga catgcgaggt atttgcatcc
     4321 cttacccccg atttccatat ttctttttcc ataacacgac cagagaacct gccaggccaa
     4381 acaccagcag caccaccggg agcagcatca ggcatgacat cagctggtcc tcgtacttta
     4441 aaaataccgg cgttttgccg agcatgtaac ccagagttgt caggatcaat acccacaaca
     4501 aaccactcat ccagttgaag aactgaaagc gcgcgttatt cagccctgat aacccggcaa
     4561 tcgtcggcag cagtgttctg acaaacgcaa taaagcgacc aattaacagc gccgataaac
     4621 cgtgtttatg aaaaagatgg tgtgcgcgtt gatgataatg cgcgggtaaa tgagatagcc
     4681 agttttgtac ggtgcgggta ttgcccagcc atcgcccctg aatatagctg acccagcagc
     4741 cgaggctggc ggcaacggtc agcagcagaa tcgtttgcgg atagcccatc gcgcctttcg
     4801 caatcaatac accgaccaac accagtaagc tgtcgcccgg taaaaaggct gccggaagca
     4861 agccgttttc aaggaacaaa atgacaaaca agacaaaata caacatgcca atcatcgacg
     4921 gattggccag ggtttcaaaa tcctgcgccc acagggcttg cagcaattgg gtcaaaagtt
     4981 ccattcatta ttcctggtga atcggttaac gccacgctgg ttttaaatga tgcactaccg
     5041 caatgttatt gctgtcttgg tatgttctgc gggtttgata catcaacaca gcctgaacta
     5101 ttccctgtta aatcgtgaga taagaaagca ttcaaaagca taaattcaaa ttgaatctaa
     5161 ttgtaacaaa ggtcaacgtt gttcgttatc aaaattacat gtccgtggag tctgattttg
     5221 ctgaatgcag gggagggagg cgagaggatt tacacaggct gacactttat acttttcgct
     5281 taccgacgag acaaacaggc gcactgtagc gcctgtcgga caggaatttc atttactgcc
     5341 gctggtggct gtatcgagat gcaccaccgg gttttcggcg aacagatagc ggtcggcatt
     5401 gaactcgaaa tcgtcgctgg tttcgttaaa taacatgatc ttggtgtttt ccaggtgctg
     5461 ccacattgcc agcttagcag catgaggatc tttgcgaatc agcgccttga ggatttgatc
     5521 gtggtcatca caccagttat cgacggtacg ggcatcaatg tgttcgtgca gttttttcca
     5581 gtacgggtta tgactacgct gggtccacat tttttccacg atagccgcca gggcggagtt
     5641 ctgcgtcgcc agggctacct gaatgtggaa ctgcaaatcc cactcggaat cacggaagca
     5701 ttgttcgccg cgcgcctgct cctggatggc catcagtttc atgatgtcct gtttcgtcac
     5761 ctgagttgcc gcgaactcgg caatattact ttcgatgagc tggcgagcct ggagcaactc
     5821 aaacggaccg taattggcga attccatatt attgtcagtc gcctgctgat ggcgcggctg
     5881 gttggaaacc acatgaatac ctgaaccttt acgcacttcg acatagcctt caacttccag
     5941 catgatgatg gcttcacgaa ccacggtgcg gctgacgttc ttttcatcgg caataaagcg
     6001 ttcagcaggc agtttatcac ccaccagata gacgccctgt tcgatgcgct ctttcagatc
     6061 agcggcaagt tgttgataca aacggcgtgg ttcagtgatt tccatatgcg ctccaggcat
     6121 gatacggcag actatatttg ttataccact tttgcgagtt tagctctact cacagtgatg
     6181 aaaaagccgc ctgagaaggc ggctttgaag ggaaactaaa acttacacga agccttaatg
     6241 ttgcggtgcg ggatcgttat gtgattcctg cgccacctca atcgccggtt tgttctgcaa
     6301 cacggtccag ataaccagcg cacctaacag gtcgaacact gccagaactg cgaacagcgg
     6361 gctgaagccg atggtgtcag ccagtgcacc gactaccagt gcaaacagcg tacttgccag
     6421 ccatgcggac atcccggtta aaccgttcgc cgtcgccact tcgttacgac cgaacacgtc
     6481 agacgagagc gtaatcagtg cgccagacag tgcctggtga gcaaaaccac cgatacacag
     6541 cagcataatc gcgacatacg ggttggtgaa cagaccgatc atacccgggc caatcatcag
     6601 caccgcaccc agcgttacga ccatcttacg ggaaacgatc aggttcacac caaaccaacg
     6661 ctggaacagt ggtggcaggt aaccaccgag gatacaaccg aggtcagcaa acagcatcgg
     6721 catccaggca aacatcgcga tctctttcag gttaaagccg taaactttaa acatgaacag
     6781 cgggatccac gcgttaaaag taccccatgc cggttctgcc aggaagcgcg gcagcgcgat
     6841 accccagaac tgacggttac gcaggatctg accaacggac attttcttcg ctgtgtcaac
     6901 ctggtgctgg gcttcctgac cattaataat gtagtcgcgt tcttcatcgg tcagatgttt
     6961 ctggtcgcgc ggatgtttat agaaaatcag ccatgccatc gcccagataa agctcaacgc
     7021 accggagatg ataaatgcca tctgccagct gtgcatcacg attgcccata ccaccagcgg
     7081 cggcgcaatc atcgcaccaa tcgaagaacc tacgttaaag taacctaccg cgatggaacg
     7141 ctctttcgcc gggaaccatt cggagctggc tttcagaccc gccggaatca tcgcggcttc
     7201 cgcggcaccg accgcaccac gagcaacagc caggccgccc cagctacctg ccagcgcggt
     7261 tgcaccacag aacacggccc acagcacagc aaacattgca taaccgattt tcgtacccag
     7321 cacatccagt acatagcccg ctaccggttg catgaccgta taagcagcag aataggctgc
     7381 gatgatatag gaatactgtt gggtggagat gtttaactct tccatcagag ttggcgcagc
     7441 tgccgccaca gtgttacgcg tcaggtaacc aagcacggtg ccgagcgtca ccagtgcgat
     7501 catataccaa cgtaaccctt taattttacg catgtaaaac ctcatcgttt tgttatatcc
     7561 gcaggaagcg cggccatcat cccgccagag gctgaagatg ttagttacga ccgggtaacc
     7621 gaaaaaaaac cggcacgccc cgaacgttgc catgaaagtt tcgtgctaat tcggcttccg
     7681 taccggttaa cccgattcat ctaacaaatg aatgaatcgg caatttgcat tccgtcgact
     7741 tatgatttgc gacggcagaa agataacttg tcatacaact ttaaaaggtg agagccatca
     7801 caaatgtgcg aatatttgta gggacattac ctgacgacgg cgaggccagt actggcgcgg
     7861 cctgcagcga gatttaccac tttgagagta atttttttaa ctacgtttat tgatctaact
     7921 cacgaaaata tcttcggact ctggaaattg gtgtgataac tttgtcagca tcgcaccata
     7981 agcaagctag ctcactcgtt gagaggaaga cgaaaatgac tccgtttatg actgaagatt
     8041 tcctgttaga taccgaattt gcccgccgtc tgtatcacga ctacgcaaaa gaccagccga
     8101 ttttcgatta ccattgccat ttgccgccgc agcagattgc ggaagactat cgttttaaaa
     8161 acctgtatga catctggttg aagggcgatc actacaaatg gcgcgctatg cgtaccaacg
     8221 gtgtggccga gcgtctgtgc accggtgatg cgtctgaccg tgaaaaattt gacgcctggg
     8281 cggcgactgt tccgcacact attggcaacc cgttatacca ctggacgcac ctcgaactgc
     8341 gtcgtccgtt tggtatcact ggcaaattgc tttctccgtc aactgccgat gaaatctgga
     8401 acgaatgtaa cgaactgctg gcgcaggata acttctctgc gcgcggcatc atgcagcaga
     8461 tgaacgtgaa aatggtcggc accaccgatg acccgatcga ttctctggag catcacgcag
     8521 agatcgccaa agacggctct ttcaccatca aagtgctgcc gagctggcgt ccggacaaag
     8581 cctttaacat cgaacaggcg acctttaacg actacatggc gaagctgggt gaagtctctg
     8641 ataccgacat ccgccgcttt gctgatctgc aaactgccct gaccaaacgt ctggatcact
     8701 tcgccgctca cggctgtaaa gtgtctgacc acgcgctgga tgtggtgatg tttgctgaag
     8761 cgaacgaagc ggaactggac agcattctgg cgcgccgtct ggcaggcgaa accctgagcg
     8821 agcacgaagt ggcacagttc aaaactgcgg tgctggtgtt ccttggtgcc gaatacgcac
     8881 gtcgcggctg ggtacagcag taccacatcg gcgcgctgcg taataacaac ctgcgtcagt
     8941 tcaaactgct ggggccggat gtaggctttg actccatcaa tgaccgtccg atggcagaag
     9001 agctgtctaa gctgctgagc aagcagaacg aagaaaacct gctgccgaaa accattctct
     9061 actgcctgaa cccgcgcgat aacgaagtgc tgggcaccat gatcggtaac ttccagggcg
     9121 aaggtatgcc gggcaaaatg cagttcggtt ccggctggtg gtttaacgat cagaaagacg
     9181 gtatggaacg tcagatgacc caactggcgc agctcggtct gttgagccgc tttgtcggta
     9241 tgctgactga cagccgtagc ttcctgtcat acacccgtca cgaatacttc cgccgcattc
     9301 tgtgccagat gatcggtcgc tgggtggaag cgggcgaagc accggcggac atcaacctgc
     9361 tgggcgagat ggtgaaaaat atttgcttta acaatgcgcg tgactacttc gccattgaac
     9421 tgaactaagg tctggggttg atatgcaata catcaagatc catgcgctgg ataacgtcgc
     9481 ggtcgcttta gcagatttgg ctgaaggcac agaagtcagt gtcgataacc agactgttac
     9541 gctgcgccag gatgttgctc gtggacataa atttgcgtta acggatatcg caaaaggggc
     9601 caacgtcatc aaatatggcc tgccgattgg ttatgcattg gcggatattg cggcggggga
     9661 acacgtccac gcccacaata cgcgcacgaa tctgagcgat ctggatcagt atcgctatca
     9721 acctgatttt caggatctgc ctgcgcaagc ggcagatcgt gaagtgcaga tctatcgtcg
     9781 cgctaacggc gatgtcgggg tgcgtaatga gctgtggatc ctgccaaccg tgggctgtgt
     9841 caacggcatc gcgcggcaga tccaaaatcg tttcctgaaa gagaccaaca acgccgaagg
     9901 tactgacggc gtgttcctct tcagccacac ctacggctgc tcacagctgg gcgacgatca
     9961 catcaatacc cgcaccatgc tgcaaaacat ggtgcgccac ccgaacgcag gcgcagtgct
    10021 ggtgattggt ctgggctgtg aaaacaacca ggttgccgca ttccgtgaaa cgctgggcga
    10081 tatcgatcct gaacgcgttc atttcatgat ctgccaacag caggatgatg agatcgaagc
    10141 cgggatcgcg catttgcatc agctgtataa cgtgatgcgc aacgataaac gcgagccagg
    10201 caaactcagc gaactgaagt ttggtctgga gtgcggtggt tctgacggtc tttctggtat
    10261 tactgctaac ccgatgctgg ggcgtttctc tgactacgtg attgctaacg gtggtactac
    10321 cgtactgacc gaagtgccgg agatgtttgg cgcagagcag ttgctgatgg accattgccg
    10381 cgacgaagca acgtttgaaa aactggtcac catggtcaac gacttcaagc agtactttat
    10441 tgcccatgac cagccgatct acgaaaaccc atcaccgggg aacaaagcag gcggtatcac
    10501 cacgctggaa gataaatcgc ttggctgtac ccagaaagcg ggttccagtg tcgtggttga
    10561 cgtgctgcgt tacggcgagc gtctgaaaac gccggggctg aacttgttaa gtgcgccggg
    10621 taacgatgcc gtagcgacca gcgccctggc gggtgcgggc tgccatatgg tgctgttcag
    10681 tactggtcgt ggcacgccgt atggtggatt tgtgccgacg gtgaaaatcg ccaccaacag
    10741 tgaactggcg gcgaagaaaa aacactggat cgactttgac gcgggtcagc tgatccacgg
    10801 taaagcgatg ccgcagttgc tggaagaatt catcgatacc atcgttgagt ttgccaacgg
    10861 taagcaaacc tgtaacgagc gtaacgactt ccgcgaactg gcgatcttca aaagcggcgt
    10921 aacgctataa agtcgaaatg agttgttaat aaaaaacggc gtttcataat gtgaagcgcc
    10981 gtttgctttt ccagacctgc taaggattta tcatgaccgc gtattggctg gcccagggcg
    11041 tgggtgtcat cgcctttctg attggtatca caacattttt caaccgtgac gaacgccgct
    11101 ttaaaaagca gctttcggtc tatagcgccg ttattggcgt acattttttt cttctgggca
    11161 cctatcccgc cggtgccagt gccatcctta atgccattcg tacgctgatt actttacgca
    11221 cgcgcagctt atgggtaatg gcgattttta ttgtgctgac tggcggaatt ggcctcgcga
    11281 agttccatca tcctgtcgaa ctactgccgg taatcggcac gattgtcagt acctgggcgc
    11341 tgttccgctg taaagggctg accatgcgct gcgtaatgtg gttttcaacg tgttgctggg
    11401 tgattcacaa cttctgggcg gggtcgatag gcggcacgat gattgagggg agttttctgc
    11461 tgatgaatgg tctgaatatc attcgtttct ggcggatgca gaaaagggga attgatccgt
    11521 ttaaagtaga gaaaacccct cccgccatag acgaaagggg ttaaacaatt aattacgcag
    11581 ggcgctattt gccagacgat cgtcttccgc ctggcaagct gccgcagtga acagcacgtc
    11641 agttgaagag ttcagcgcgg tttcgcaaga gtcctgcaat acgccgatga taaagccgac
    11701 ggcaaccacc tgcatggcga tatcgttcga aataccgaac atattacagg ccagtgggat
    11761 cagcagcaga gacccccccg ccacgccgga tgcgccacag gcacacagag aagccactac
    11821 gctcaacagc agcgctgtgg gcagatcgac cggaataccc agcgtattaa ccgcagccag
    11881 cgtcaacacg gtaatggtga ttgctgcgcc cgccatattg atggtggctc ccagcggaat
    11941 agaaacagaa taggtatcgc gatccagatt cagcttttca cacagcgcca tattcaccgg
    12001 aatgttcgct gcagagctgc gggtgaagaa ggcatacaca ccgctttcac gcaggcacag
    12061 cagcaccagc gggaacgggt tacgacgaat tttccaccac accagcaatg ggttaaccac
    12121 cagcgccacc agcagcatac agccaaccag cacgaccagc agttgcgcgt agccccacag
    12181 cgtggagaaa ccggtggttg ccagggtaga agaaaccaga ccaaaaatac cgatcggtgc
    12241 gaagcgaatc accagtttca ccataaaggt gacggcattc gacatatcgt tgatcaggtt
    12301 tttggtggtc tcgttaccgt gacgcagtgc gaaaccgagg ccaattgccc acaccaggat
    12361 cccgatatag ttacctttta gcagcgcgtc aatggggttg gaaaccatgc tcattaccag
    12421 cccgcgcatt acttctacaa tgcctgacgg cggcgaaata tcacccgcgc tactggacaa
    12481 gtgcagggta gaagggaagg caaaactgaa gattactgcg gccagagcag cagagaaggt
    12541 gcccagcaga tagaggaaca aaatagggcg gatattggtt ttctgcccgt gctggtggtt
    12601 agcaatagat gccattacca gcatcaacac caggatgggg gcaacggctt tcagtgcgcc
    12661 gacgaacaaa gtacctaaca gaccaacagc ttccgccgcg ggttttgaga tccatgccag
    12721 aagaatcccc agaacaaggc cgaccaggat ttgttttacc aggctgccat gagccagacg
    12781 ccggaatagc cccggtgaac gttgcgtagt catttttcga tcctttcatt gtgttgtcgc
    12841 acatccttgg tgcgttctgt cgacgcatct ttcaggatgt aacaaagtgt ttgcaaggtc
    12901 gagtataagg aaagttacag cttcgggaag cgaaaaatgc tggattttaa agagaggtca
    12961 tattttgcaa cttaatggtt acatttattt gacataaaca gagggaagga ctggctacta
    13021 aggctgatta gataacatat gggcaaaact atttggaata tgagaacggt tgcgctcctt
    13081 taatgtgcta tgccatataa agccactggc gcagggagcg cgcagggggc ggccaatcgc
    13141 cgccgccccc tgcaccccca ggctctggcg aacaaaatcg ccgctgcgcg gttccctcag
    13201 cttatccctt gcggctaccg ggtcgggcgc aagggaacgt ccctgtaaga tgcgccctcg
    13261 ccgcgcatcc atgcgcgtcg ccccggcctc cagggaacgc ttcggcgatt ttgacgccac
    13321 caacacccgt gcggtctttt tgtaaagagc tataagccga ttacataatt aatagctcac
    13381 ctttgtctgg aatgagttat ctctagtaat ggcttaatat acagcgaatc attctccccg
    13441 ctgcttatca tgccgataat tcacccaggc gttgataata aacgtcatca ccagaatgcc
    13501 aaacaccacg cccagcgaga cggcgatcgg aatatggtag aagtcgacaa tcagcatctt
    13561 gataccgata aacaccagaa tcaccgccag accatatttg agcatcgaga aacgctctgc
    13621 cacacctgcc agcaggaaat acatcgcacg caggccgagg atcgcaaaca ggtttgaggt
    13681 cagcacaata aacggatcgg tggttacggc gaagatagcc ggaatactat ccacggcgaa
    13741 aatcacgtcg ctcaactcca ccagaatcag caccagcatc agcggcgtgg catacaacaa
    13801 accattttta cgcacaaaga aatgctcgtt gtcgatggtg tcggtcatgc gcaaatgacc
    13861 gcgtagccag cgcaccagcg gcttgtcgcc aatgcctgat tcatcttcat gggcgagcgc
    13921 catcttcacg ccggtaaaca gcaggaaggc accgaagata tacaggatcc agtcgaactg
    13981 tgaaatcagc cagctgccag tgaagatcat gatggtacgc agaacaatcg ccccgagcac
    14041 gccatacacc agcacgcggc gttgtaatgc cgccggaaca gagaaatagc tgaacaacat
    14101 cagccagaca aagacgttat cgaccgccag cgatttctca atcagataac ccgtgagaaa
    14161 ggccagtgcc tgtggatcgg caacggcgcg accttcggtt tgcaccagat accaccagaa
    14221 agcggcgtta aacagtaacg acagcgtcac ccagaccagc gaccaggccg cagcctgttt
    14281 catggtcatg gcatgtgccc cacgacgccc ctgcaacaac aggtcgatag ccagcataat
    14341 ggtgacaaca acagcgaatc cgccccatag caacggcgtg ccgacagtat tcatagaagt
    14401 tccttacaca taaaaaaacg gccaacgtcg gaagacgtca gccgctgctt tttatgcata
    14461 gacctcgcct tccggcaagg tctcacttac aacaaaaaag gaatacgtct gcgtattcct
    14521 ttcgggttgc ccggtgaccg gatgtggttt ttcacacatc gtaatgacga tcgaccggca
    14581 atgaagttac tcccctttgc aggtaacaaa gtatgacaga ccattcagtc agtcaatggt
    14641 ctgttacata ccttttacac tgttttactt tggcaattat agttttacgc tatctgccgg
    14701 aaaaatcacc ccagtctggc ggcggatctc ggtcagcagt ttagcggtga tgcgactgac
    14761 cgccagccct ggatgatcca ccagatgctc atccaccagt tcagcaaaca gctctgcttc
    14821 gtagagcatg gtattaatat gctgcggttg ggtcagatcc tgcatctggc tgccacgcgg
    14881 cacaaaacac actttctggc attcagacag tttttcaatc accagcgatc ccgcttcgcc
    14941 ctgaatctcg cttgccagga cagaatcgct cacttttgag tgctgcaacg taacgctgaa
    15001 atcaccgtaa tccatcacca ccacgccgtg ggcatccaca ccgcttgcca gcaaactggc
    15061 ggttgcctgc acgtttttcg gttcaccgaa taatgccacc gccgacgcca gacagtaaaa
    15121 gccgatatcc ataattgaac cgttagagaa tgctggatta aaggtgttgg gattctcacc
    15181 atccaggtaa cgttgatacc gcgaggaata ctggcaatag ttgaaaaaga ctttacgcag
    15241 tttgccgact ttcggcagcg cctggcgcaa cagatgaaag ttcggcaggt aggccgtttt
    15301 aaatgcctca aacagcacca cctgattttc ccgcgcacag gcaatggcgg catccacttc
    15361 cgccagattc gatgccagcg gcttctcgca aatcacatga attttgtgac taaggaaaag
    15421 ttgtgtctgg gaaaagtgca gggcattcgg gctggcaata tacaccgcgt caatggcatc
    15481 gctttccgcc atcgcttcca gcgacgtaaa cagatgctcg acagaaaaat cattggcaaa
    15541 gtgctgggcc tgttcaaggc tgcgggaata tacggcggtt aacttgtatt taccgctctc
    15601 atgggcggcc tcgacgaact ggcgagtgat ccagttcgta ccaatcacag cgaaacgtat
    15661 cataacgcgt ggatactcca taaagggatt ctgccgtcaa tgtagcacgc ctttttcatc
    15721 ttcgacgtgc gcagatacgc attatttctg cgccggacag ccatgctcac taaacggccc
    15781 aatcatgacc gatggcccga gatatttcgg ctggcgatgg aggatttttg cgtcaatcac
    15841 cgcgccgacg cgagcgagtt tctcccgcag ttgagtataa aaaccgtgct tcatattgag
    15901 gtctggcgcg ttaaaaccga tagcgtcgat gccgtactgt tttgccagcc agatggcgcg
    15961 ttggttatga aattcttgcg aaataatggt gatatggttt tccccaaaca cctttttggc
    16021 acgcaccacc gaatcaagcg ttgagaatcc ggcatagtcg cagaagatca ctttcgccgg
    16081 tacgccttta gcgatcaaag cctgctgcat acctgaggct tcatcgtaat ttttacgccc
    16141 gttatcgcca cttaccagta gccatttcac ttttccggcg tggtacaacg ctgctgcggt
    16201 atcaatacgt cgggtaaagt agcgattacc cggcctcgcc cccagcaata aacccacgtt
    16261 acgcgccgga actgcgctga catcgctcca ggtcagctgc ttgctggtgt tgaccataac
    16321 gcgatcggca ataaaaatgg tcgctcctgc tataagcaac aataaacagc caattttaag
    16381 agtccggcga gaaaaacaaa tacggagaag aaggcgggcc tgcatggtcg ttatccttaa
    16441 cgaaaggggg gaggtcgatt ttaggcgcta taggaagaaa agataacgcc tgacatcatt
    16501 ttcagatttt gtcctgccca agcgacaact gcgttagcca cagccgggtg tcgaactcaa
    16561 gctggtgata ctgcggctcc atatggcagc acaattgata gaacgctttg ttatgctctt
    16621 tctccttcag gtgcgccaac tcgtgcacca cgatcatgcg caaaaacggt tccggcgcgt
    16681 tgcgaaacac ggtagcgacg cggatctccg cctttgcttt cagcttgccg ccctgcacac
    16741 gcgaaacggc agtatgtaat ccgagcgcgt ttttcagcac gtggatctta ttgtcataca
    16801 tcactttagt gatcggcggg gcattgcgca gaaactgatt tttaagatcc tgagtatatt
    16861 gccagagggc tttatcggtg gcgtagtcgt gtgttcccgg atagcgtttt gccagcacat
    16921 ctcccagacg ctgttcgtta atcagcgttc gcacctggga aagtagctgc tcgggataac
    16981 cctgaagata agtaagattg ctcataaccg cccacgaaaa agagaaaaag ggtatactca
    17041 cgcacccttt tcaggggcaa cgtcgaaatt ttatcattca ggagggccga tgagccactt
    17101 agacaacggt ttccgttcac tgacactaca acgttttccg gcgacggatg acgttaaccc
    17161 actacaggcg tgggaagcgg cggatgaata tttgctgcaa cagttggacg acacagaaat
    17221 ccgcggcccg gtgttgatcc tgaatgatgc ctttggtgcg ttaagttgtg cgctggcgga
    17281 acataagcca tacagcattg gcgactcata catcagcgaa ctggcgacgc gcgagaattt
    17341 acgcctcaac gggattgatg agtcgagcgt gaagtttctc gacagcaccg ccgactaccc
    17401 gcaacagccg ggcgtggtac tgatcaaagt accgaaaaca ctggcgttgc tggaacaaca
    17461 actgcgtgcg ctgcgcaaag tggtcacgcc ggatacacgt attattgccg gtgccaaagc
    17521 ccgtgacatc cacacttcca cgctggaact gttcgaaaaa gtgctcggtc cgaccaccac
    17581 cacgctggca tggaagaaag cgcgcctgat taactgcacc ttcaacgaac cgccgttggc
    17641 cgatgcgccg cagaccgtta gctggaagct ggaaggcact gactggacta tccacaacca
    17701 tgcgaatgtt ttctcccgca ctgggctgga tattggcgcg cgcttcttta tgcaacatct
    17761 gccagagaat ctcgaaggtg agattgtcga tctcggttgc ggtaatggcg ttattggcct
    17821 gacattgctc gataaaaacc cgcaggcgaa agtggtgttt gtcgatgaat caccgatggc
    17881 ggttgcttcc agccgtttga acgttgaaac caacatgccc gaggcgttgg atcgctgcga
    17941 gtttatgatc aacaacgcgc tctccggcgt ggagcctttc cgttttaatg ctgtgctctg
    18001 caacccgccg tttcatcagc aacatgcgct gaccgataac gtcgcctggg agatgttcca
    18061 ccatgcccgc cgctgcctga aaatcaacgg cgagctgtat atcgttgcca accgtcacct
    18121 ggattacttc cataaactga agaaaatttt cggcaactgc accaccatcg ccacgaataa
    18181 taaatttgtg gtgctgaaag cagtgaagct ggggcgtcgt cgataagatt gggagttacc
    18241 tgccggatgc ggcgtaaacg ccttatccgg cctacgttca gcacattacc cccaggtcgg
    18301 ataagacgcg gcaagcgtcg catccggcaa tgtctgccca gttctggaat cgcgcttcca
    18361 taaaacaaaa aatccctgtc accatcctct acattctctt gtttagcgtt tttctacgtt
    18421 tatgctacat acgtgaagtt atgacgcata aagaatacga tttctttacc gccgttcatc
    18481 gtactaaggg gaaaaaatga ttgcgattgc cgacatcttg caagcaggag aaaagctaac
    18541 tgctgtggca ccttttctgg cgggtattca gaacgaggaa caatacgccc aggcgctgga
    18601 actggtcgat catctgctgc tcaacgatcc tgaaaatccc ttgctggatc tggtgtgtgc
    18661 caaaataacc gcgtgggaag aatcagcgcc cgaatttgct gaatttaatg ccatggctca
    18721 agccatgcct ggcggtatag ccgtgattcg tacccttatg gatcaatatg gtttaaccct
    18781 ttccgatctt ccggaaattg gcagtaaatc catggtgtca cgcgttttga gcgggaagag
    18841 gaaattaacg ctggaacacg ctaaaaaact ggcaacgcga ttcggcattt ctcccgcctt
    18901 gtttattgat taacgcgacg tgccggatgc gtcgcatccg gcgcttcaaa caacgattta
    18961 aatctccagc gccagccgtg ttccctgggc aattgcccgt cgtgcgtcaa gctccatagc
    19021 tacatcgcag ccgccaatta aatgcacagt tttcccgcta tcaatcagct gttgcgccag
    19081 cgcgcggttt ggctcttgcc ctgcgcagat caccacattg tccactgcta atacctgcgt
    19141 ttcgccgttg atcaccacat gcagcccgtc atcgtcaatc ttctgataac ttacgcctgg
    19201 gatcattttc acaccccgcg agagcagggt ggtgcgatgg atccagccgg tggttttgcc
    19261 taacccctgt cccggtttgc tggctttgcg ctggagcatc acaatctgcc gtgggctacg
    19321 cgggatctgc attccctgcg ggcttaagcc accagcctgt tgcaggctgc tgtcgatccc
    19381 ccactcatta cagaactcgg caatgttctg gctggtggac tcacccggct gacttaaaaa
    19441 catcgccgta tcaaaaccaa tcccgccaca accgatgatt gctacctttt tgcccacagg
    19501 cgctttgtcg cgcagtacgt cgagataact caacaccttc ggatgatcga ttccgtcgat
    19561 aggtggaatg cgcggtacga tcccgctagc gaggatcgtt tcatcgaacg actgtaactg
    19621 atccgccgtc acggtgtgat tgagtttcag cgtcacgcct gttacttcga tcatccggcg
    19681 gtaatagcgc agcgtttcgt aaaactcctc tttgccaggg atctgtttgg cgatattaaa
    19741 ctgaccgccg atctcactgt gagcatcaaa caacgttacc tgatgaccac gtgccgccgc
    19801 gttaatggca aacgccagcc cagccggacc agcgccgacc accgccagat tttttttctg
    19861 cacggcggga aggatcggca ttttggtttc gtggcaggcg cgaggattca ccaggcacga
    19921 ggtgactttg ccaacgaaga tctgatcgag gcaggcttga ttgcagccaa tacaggtgtt
    19981 gatctcatcg gctcgccccg attgcgcttt tgacagcagt tccgcatcag caagaaacgg
    20041 tcgcgccatc gataccatat cggcatcacc gcgcgagaga atgtcgtcgg caacctgcgg
    20101 atcgttaatc cggttggtgg tcaccagcgg cagcgagacg tggcttttca gtttgcgcgt
    20161 gacccagcta aaggcaccgc gcggcacggg cgtggcaatg gtcggaatac gtgcttcatg
    20221 ccagccaatg ccggtgttga taatggtcgc gcccgccgct tcaatggcct gcgccagccc
    20281 taccgtttct gcaaaagtac cgccgccttc taccaggtcg agcatcgaca gacggtagat
    20341 aataatgaag tcattgccga cgcgttcgcg cactgctcgc accacttcaa ctgcaaagcg
    20401 catccggttg cggtaatcgc cgccccactg gtcgctacgc tggttggtgc gcagggtcag
    20461 aaactcgttg atcaaatacc cttcggaacc catcacttct acgccgtcgt atccggcctc
    20521 ccgcgccagt tgcgcgcagc gggcgaaatt gtcgatcagt tgcaggatct cttcatggct
    20581 taactcatgg ggcacgaaac ggttgatggg ggcctgcaat gcggagggag cgaccagatg
    20641 cggttggtag ctgtagcgcc cggtatgcaa aatttgtaag gcaattttgc cgccttcctg
    20701 atgtacggct tcggtaatgg tgcgatggtg tgggatctgg ctggcgtcgt tgagcattgc
    20761 gccgccttcc atgccaacgc ctgttaaatc tggtgcaatg ccgccgctga caatcaacgc
    20821 cacgccatga cgggcgcgtt ccgcgtaaaa cgctgccagc cgttcagcgc cgtccgggta
    20881 ttcctccagt ccggtgtgca ttgaacccat cagtactcgg ttttttaacg tggtgaagcc
    20941 taaatccagc ggggcgaaca gcgacgggta gctcataaaa ttgtccagta tgtaaaataa
    21001 ttgttatgtg gtcggatgag ttctaattta gccgtcgctg gagaaaaggg aaaaggggag
    21061 tgcggggttt gtgatgggat tcaaaaagaa tacgcccgtt cgtcatgccg gacggcggta
    21121 atacagtcgt ccggcatcgg gaaaaattac tgcttacgga aaaaatcgtt atacaacata
    21181 tacaaatgcg cggcactcca ggagaaattt ggtgcgcctt gctgtgcgcc agtcagcggg
    21241 ttgtaattct cctgaattgg gccatcggcg gttaatcctt tggcgtgccg gaagaacgta
    21301 tccgccagct tcagggcatc atcgcgataa ccgtaacgct ccatcccttt cagaccaaac
    21361 cagaactgat ccacccacac gcgcccgcgc cagtaaatat ctgctccaaa ggccggattg
    21421 gttaacgccg ccgttcccag cggtacaaag gtgttgaact ctttaggatc gagcatcacc
    21481 ttcaccaccg cgtcggcgtt ggcctgcgtt gccgcaccgt taaacagcgg cgaccagcct
    21541 tccggccctt tgccgcgctc gacaatcggt ttgcccgcgc agccgttcgc cagtggttta
    21601 tcttcaatgc gcacgtcata gtagaactgg gtagtcgggt cgaacataca ggtgttgatg
    21661 tagtccgcga gctgttgtgc taactgacga tagcgtttgg cctcttccgg cttaccgaga
    21721 atcgtcgcca tctccgccag ataatggtta tcgctgtaca tatagctggc ctgatccacc
    21781 gactcctgca atagcgagta gcccagcaac gttccgtcct gactgcggtt ttcggcgaat
    21841 ttcaccgtcc agtcgctacg tttgccgccg ttagcgacat atttatccag ctgttctttg
    21901 tcgataaacc caaagacggc ggcatcatca cgccccgatt cccacgacgc cgcaacctgt
    21961 gccgggattt ccagactgtc gtactggcct ttctctacca cgcgggcgta gttgttcagc
    22021 ccagacagcg tctctgcttt gttgcctttc ttcaccgtaa acagcatctc gccgctctca
    22081 gtgttgtggg ctttgtcgcg ggtcgcgcca tattccggca cgccgttgcc gttgtgatcg
    22141 cggttacgta gccaccagtc gtgataggcc accagtttcg ggtacatctc tgccagccag
    22201 gttttatcct gggtgacgtt atacacttcc attactgacc aggcggcaag gctgggtttg
    22261 gtattacgtt tgttccagtt gccgccatcg ccgccacgct cggggctaag gttccatgct
    22321 atcaggtcgg ggacaaagcc cacatcctgc ggacgcacgc ggtcgcctgg ctggatctgc
    22381 caggagaaga ccgcgcggat attctctttg gcgatatccg gattgaaatg cgccatcgca
    22441 aatgcctgct tccaggtatc ccacggccag gtctgattgc cggagaacca gcgcccggtc
    22501 accgacggcg tgacggtgtt atatttcacc gcccccccag gcgagcgcca gttaccgttg
    22561 agcgtttcga tcgctttcac cgcgacgcgc gtctgttccg gcgtcgcatc cggattggtt
    22621 aaccctttct tcagatactc ttcccagcgt tgctgcgagg cggtgagata aaacgccgga
    22681 cgcgccagaa tatcgcggat ctgcatttgc tctttgctaa cttcctgagc ggtcagcagg
    22741 tgggagtagg tggtatagag cgtggccgaa ccgttgatat gcgccttact ggtaaagcga
    22801 ttaccgttga tttcagtctg cactggcagg gatttatgca cctgatattc tgattcaccg
    22861 gaggtcagca gatcccaggt ggcgcgcact ttgccaaagg tgactttcag gccatcacgg
    22921 gtggcgctga ttttgcgctg atagtcaggg tattcgccag caatggtttt atcggaaagc
    22981 ggtttccctt ctttcgcttc cagtttttcc agcagctcgc cgtcccacac cagatccagc
    23041 ggtttattgc tgatgatttt agtttccagt agtgacgtgc gcggcgtggc gaagcgcaga
    23101 gtcatttcga cctgcacatc ttttgctgtc agtttttgca ccagcgcgcc gggaatactg
    23161 tatgcctcca gcgtgaagtc gactttcttg ccgtcttgcc agacggtcag acgatcgaaa
    23221 ttgctggcca taaagttgat gtattcttcc gtcagcagcg caacgcctgg aaaaccgccc
    23281 atggtgttcg ggccgtctgg caacagatga ccatgccagg ctccgagatc gaaaaacgga
    23341 ttaaaacgct ggtgatcgtc gtaatcgtaa tccttcatgt actgcggcgc gccagtacgg
    23401 ttaatcacgt ttttataatt gtcggcgtta gcggcatggg cggaaaaact tatcagcaga
    23461 gcgcaggtta ctggcgttaa aatagttttg attttcatga aattacagtc cttattacca
    23521 gtagaaatat tgcatcagga agagttggtc gctgggatcg tgagaggcat ttttcatgta
    23581 gaagcggcta tcaaaagccg ttgcgaaacg tccgccgccg tattcgtacc agataccaaa
    23641 ctgcatgaaa gaggtggttt catcgtcagc gtggtcaggg cgatgtttgg cgtaggtgta
    23701 agcggtggag agataaatgc cttttaacgc ttcatacatg gcgctcacca tcatcccgct
    23761 ttcacttccc gatacgtcca gcccgtcggc gcgtccggta tggtgccagg cgcgagcggc
    23821 aaagttaggg gtaagcaggc ccgtcagata gagttggcct gtgccgtcgg tgatttccag
    23881 accgttcatc caggtgatgc cttcggcaag gtcatactgg atgtaaccgt tcatcattgc
    23941 cggataggtg tatttgtcgt cgtagcgatc gtacttgcca aaatgtagcc aggctttgct
    24001 ttcgtcatga tgtccggcag gtgtggcggt aacgctataa cgaaagttgc cgcttaagtt
    24061 ctgcaccttc agcgcggtca taatgtcgcg ggtattggga atgacgtagc ccagatcggg
    24121 cgtgaaatcg ccccaccatt gcaggtcatc cagcgaggaa tcctgacgca gactgagcat
    24181 gatttcggtg ccatcattgg tacgataacc gccatagaaa cggttaatcc caccttcgaa
    24241 accgccccag ctatggtcag gcacccaggc attaccgtcg tggtcggcct gcacggtcca
    24301 gccttcgccg taaatcaggc cgaaccagga tccgtacttc atctccaggc caccttcaat
    24361 ataggtgccg tcgctgtatt tatgtttatt ctcaccctct aaatccatat acccaccaac
    24421 ccctaattcg ccgtaaaaac gaaatgaagg agaagatgtc gaggtcattg cgggggtttt
    24481 tgtgtcgtca tgttctgccg gacgtgcttc atcagcggtc acagaaaagg cgtagcaaaa
    24541 cggcagcgca agtaatgctc tgcatggtgc agtgattaat ttcattgtgt taatccatga
    24601 gctaaagaaa aagtacagcc ttttatcgtt gtaattataa aaatataaag gcagaaacta
    24661 ttttccctct ccccatccag gaagagggaa aatgcctgct cattttttat tattctgctt
    24721 aatcaacatt gctggctggc gtcgcttcaa tatggcgctc ttcagccgtt aatcccttta
    24781 tatatttact gtatttccac cacgcaaagc cgaggaagat aacaataccg ccgacgttat
    24841 aaaaaatgat ggtcagaata ttcgcgccag tcgggaatgt cgaagcgaca aaccctacgg
    24901 caaatatcgc aatcagcatt gaaacaacga taataccggt gcggcgggag cccatacgga
    24961 aatcgcgtgg caggtgatct aatttggcgc gtaaattcag gtaagccagc atgatgaata
    25021 acggcggaag catggacgct gcggcggtca tattaataat ggtattcatc agatcttgca
    25081 cggtattgga accgagcatc gggataatca tcagcgggat gacgatcagg aactggatcc
    25141 acgctgcgcg cgccggaacg ccgttttcgt tcagttcgac ggttttctta ccaaagatgc
    25201 cttccgggat ttcggagaag aaaattttca ccggcgttgc ggtccacatc agcagggaac
    25261 cgaacatcgc ggtaaaggac accagaccga caaagcgatt catcaacgct tccggcagac
    25321 caaaatacgc cgccatgccg tggaatacct gcaccgatcc gccagtaaat ttcaactctt
    25381 tgctgctgac gaagacgtta atcagcaccg aggaaacgga atacagtacg ccgataaaaa
    25441 tcccggcgag gatgatcact ttaacgaacg atttcgaacc gcctttgacg tcgttaacgt
    25501 acaccgcgac ggactccgcg ccacctgcgg ccataaagat ccaggtggta acgccgagga
    25561 acgcccagtt gaagttcggg atcatcgcgt caacggtgat cggatcggca ggctgtacgc
    25621 cgccaaccag tgccgtacct gccagtaaaa tgtaggagag cgttaacagc agcatcagcg
    25681 ttgaagtgac ggaggtaatt ggccccagca ttttcgcccc gttggtggaa acccaggtgg
    25741 agaaggcgaa cagcaccata ctgataatgg tggtggcaac cggcgtcata atatattcgt
    25801 agccgaggaa ggcatacgaa gcataagcaa taacgcgcgg caacagtgag gtgaaaaaga
    25861 acaggtttac gaaccagtag gtataggcag taataaatgc ccaacgcccg cccagcgaac
    25921 tttttaccca cgcgtagaca ccggcttctg agtttttatt taacgaaaca aattctgcga
    25981 tgatcagaca gaagggaata aaataaaaaa tcgtcgcgag gaaaaacatc ggtgccgagg
    26041 ccagtccaag ctcaatatta ttattgataa cgttgttaaa gctgtaaacg gcggcaaaag
    26101 tcagcgagag caagccgaat ttgccgattg tattacgttt ggtatcagac ataacacatc
    26161 tccgaaaatg agtagggtag cggtggcggc aaactccttt tgccgccgta gttgttattt
    26221 gttatggaaa taaccatctt cgatggtgac tttgagaacc acttttttga ccgcgttatt
    26281 gcagataaac cgatatgctt catggatatc gtaaataacg atttgccctt cgtggacctc
    26341 tacggtttct ccgcagcctt ttaaatattc acggtcagtt tcatcacgat aatattccac
    26401 gacctgtaat gtctctttcg gcgcatattc aattttttgc tgcccttgca ggtagtaatg
    26461 cacttcaaaa tagcgtcgat gcccggtaaa tagcgcatcg gtcgcggagt ccgtctccac
    26521 gcggtaagtc aatgagtcac cgatggagtg ggcgacgcca ggctgaatgt tgtcgatatt
    26581 ttcaatcgct tcaacgcagc gttgccactt cttgccagag gcgtaaatct ggcggaactg
    26641 ttctaagtta tcgatgatcc tcattgctta ttctcgctgt gcaaattcgt ggaaaagaac
    26701 cctgcgccga actcatacga cgccaggctt tgcgcggtag cttctccgcc agaaaccggc
    26761 agcaacgtaa agccgtagct gaagtcacgg aaccagacgc gccaggagtc cagcacctcg
    26821 ctgccccagg agttggagcc gaggccaagc agctggtggt cgagattcag ggtgatgtca
    26881 tcactgcgct gcaactcgtt acagtgctgg gcagcgtgga tgttttcctg ggtatagcgc
    26941 caggcgctga agttaatggg gcgctgcgga acaaccagca gaccattgcc gtggcggtta
    27001 gtcagtgccg tccagcggac atgctgacgg ttgccgttgt tctgtgggaa gggatagttc
    27061 tcgaacatgg catcgacggt gctgcgccag atatcgatga tgttagcctg ctggctgtcg
    27121 gcgtagtttt cgcccggtcc acgaccgtaa tacgccacct ggtcgtattc gccgttaatc
    27181 cccatggtga aaccgatgca cggaatgatg tgcggatagt cgccgtaacg ctcgccggaa
    27241 agcgccacgt taacctggcc atcggcagcg atgcgccaga tgtaggtgca gcgcatcccg
    27301 aagtcaaaca ccggtggggc aataaccgtg cggctgatga tcaacacttc accatcgctc
    27361 tgttctacgg caaagtcgcg cagatgttcc tgcatgatct gcaaatgatt cggctgccac
    27421 agcccttcgt actcctgctt gtggttgtcg atcattggct tgaagaagtt gatttttggc
    27481 tcgcgggtta gcagcgactc gccattcacc tgccaggatg tcggtttacc actcgttttt
    27541 gagaaggtga tcgcaaagtt gtagccgcga acagtacagc tcaaacgatc gtcttccagc
    27601 gtcagcggac gggcgttatt cggtgcgaaa ggtacaggct gcgcggtgtt ttccttcagc
    27661 gggaactgat aagtggcgat agaatgcccg gcttcgctgt agcgggtgcg ggaatctttg
    27721 gtcaccgtaa tgttgagaaa cgcttcgcgg gcgtccagct gtggcagcgt gatctgtaag
    27781 ggggcttcgc tgttcggcgc aacgtcgcgc agtttaatct gttgcgtcgc gagcgtttca
    27841 ccttcggcgc gcacctctgc gtgcagggtg tagtcatcaa gcgtggtaaa ccacagatta
    27901 ttttcgactt tcaactcgcc gcgagtcaga tccagcgcgt ggatttttac tggcgcgata
    27961 acctgtttgt actctttcag gcccggtccc ggcgtctgat cggaatagat caaaccatca
    28021 agacagaagt tatagttgtt gggatagtcg ccgtagtcgc cgccgaattt ataccagaca
    28081 ttgccgttgt catcctgcgc ctggatcccg tggtcgcacc attcccagac ataatgtccc
    28141 tgaatgcaat catgcttata gaagacgttc tggtactccg tcagcccgcc cggtccgttc
    28201 cccatcgcat gagcatattc acagatgatg cgcggcttcg gatgcgggta ttcaccaaac
    28261 tcattcatca gcggcacgcg ggtgtacatg gtggaaataa tatcgaccac ttcagcatcg
    28321 cgatcttctt cgtaatgcac cagtcgcgtg tcatccagcg ccttcgccgc atggtacatc
    28381 gcgcggatgt tacagccata gccggattca ttgcccagcg accagatgat gatcgacgga
    28441 tggtttttct gcgcgtgaat atggcgaaca atgcgctcga cgtaaacttt ttcccactgc
    28501 ggatcgtcgg taatacggct gatatcgccg acattagcaa agccgtgcga ttcgacgtcg
    28561 gtttccgcca tcacaaacag gccgtagata tcacacagtt cgtaaaaacg tggatcgttc
    28621 gggtagtgag cggtacgcac ggagttgatg ttgtgctgct tcatcaactg gagatctttc
    28681 tcgacgcgat ccatcccaac ggcgcggcct ttgcgatgat cgttgtcgtg gcggttgacg
    28741 ccgtgcagca tcacataacg gttattgatc cagaacagac cgtcgcgcac tttgatatca
    28801 cggaagccaa ctcgttgtgg caccacttcc agaacgttgc cgtcggcgtc tttcagcgtc
    28861 atgaccagat ggtaaagata aggggattct gctgaccatt gctgcggctg ttcgacagta
    28921 aaagcaaaac tggcgctggt cagtttttca attgccaaat gatcaatggt gctgctgtgc
    28981 accacgtgtt cgccatcaaa cagggtatat tccagcgtcg tgacgacagg ggaggcggcg
    29041 agattttcca gcaccacttc gcaggaaagt gtggcatcgc aataggcttc gtcaaagtcg
    29101 gtacgaacgg tgaaatcgtt aatatgcgtg agttgttttc cgatcagata aacatcgcgg
    29161 aaaatccccg ccgaccacca catatcctgg tcttccacgt aggtagagtc cgcccactgc
    29221 atcacgcgca cacacaacag gttgtcgccg gttttaacca tcgcgctgat gtcaaactct
    29281 gcggtcaggc gactgccttt gctgaacccc acatactgac cgttaacgta gacttcaaaa
    29341 taggtttcga caccgtcaaa tttgatcagc gtctgtttac cctgccagcc gtcgctgagg
    29401 gtgaaaatgc gttgataggc accggttggg ttatcgctgg ggacaaacgg cacatcgatg
    29461 gggaacggaa aaccttcgtc ggtatattgc agtttgccgt gaccttccat ttgccacatg
    29521 gcggggacgg taatatgccc ccagtcagcc attaactcag aggtgaaggc ttctggcact
    29581 tgcagcggat ggtcaaaaaa gtggaaattc cactgaccgc ttaagggcag aaacaggctg
    29641 ctggtttcac gggcaaaagt acgagcctgc gcaacagaat catatgaaaa aaagtacgca
    29701 cgcggcgcaa gtcggttttc gtgggtgagc tgaatgtttt cccagcgatt catagggcct
    29761 cccggtagag agaaatcgga accatttatg gtgatggtag tttgtgtaat tgctggggag
    29821 tttagtaaaa attttactaa aaagtagcgg ataaatgcat ttactttaac tggatcacga
    29881 aaataaacgg cagcactgtc cggcaaaaac gattgccgga cagcgtgcaa ggggatttaa
    29941 cgggtcgtgc cgcgcagttt taatttgctg ggaacgaaga ccaacagcgg cagcgcgcga
    30001 ccatcgcggg ctttttcata caccaggtta acgccctgac ttcccatcat ctcggaatgg
    30061 atgcgcacgg tggagagcgg cggaaaggta aatcgcgcgg tggggatatc gttaacgctg
    30121 ataagcgaaa tatcctgtgg gatgttcagg ccacgttcat gaattgcccg cagtacgccg
    30181 atagcaatgg aatcggaagc aacaaacagt gccttcggat agtcttcccg cgccagcatc
    30241 tgttttgcca gttcatagcc cgacgaactg gaaaaaccgc cgcgccagat atcctcttcg
    30301 cgtaccacgt gtttcagacg accatattcc gcaaaggcga cctcacgaat atccgccttg
    30361 ccaggcaagt cttcaccgcc aataaaacca atacgattaa cgccctgatt gatatagaaa
    30421 tcgatgattt ctttactgat acgtgccaga tcgatatcca ccgcatcgta accgctgccg
    30481 ggttcgtgaa agtcgataaa acagatattg tcggtcaacg cacaggcggc ggcgcgcagg
    30541 gcggacgtgg gtttgccgac aattaaaata ccggtgacgt ttttaatgtc tggtaagccg
    30601 ttgtgttcat aacagttggt cagttcgatc gccagctttt cgcactgagt ttcaatgccg
    30661 tggcggatcg ccagatagta aggatcgttg atctccagct cctgttggta gctgtagata
    30721 gccagaatat ggtgttggtt gactgcgcct gtctggagtt tacgggcgct actggtcttg
    30781 tactccagct tttcggcgat ctcgagaatg cgatgtttcg tctcttcttt cacattcaat
    30841 gtcggatcgt cattcaagac cctggatact gtcgccaggg atacgccagc ttcgattgcg
    30901 atgtctttta gtgttgccat gtttaccttt tttactttcc ctgatgtgcg cccccattgt
    30961 agaggaagtg cttatcgggt cacctgtttt cagtaaataa agcgcgtttc tttgacgctg
    31021 taacggtaac acagcggctt gcgaatgtga tcacgcctgc attctccatt tttagcctac
    31081 aggattgaga aaaacttttg ctatagttta gtaaaatttt actcgaaggg aggaaacaat
    31141 ggaaaccgtg gcttacgctg attttgcacg tctggaaatg cgcgtcggaa agattgtgga
    31201 agtgaaacgc catgaaaacg ccgacaagct gtacatcgta caggttgatg tgggggaaaa
    31261 aacgctgcaa accgtgacca gcctggtgcc gtactacagc gaagaagagc tgatgggaaa
    31321 aacagtggtg gtcttatgca atttgcaaaa agcgaaaatg cgcggtgaaa cgtcggaatg
    31381 catgctgttg tgtgcggaaa ccgatgacgg cagtgaaagc gtgctgttaa cgccggagcg
    31441 gatgatgcct gcgggcgtac gtatcgtata aaaagatcgg atggcgacgt cgtatcgcca
    31501 tccgatttga tactacgctt cttcgacact gactcgcatg gccgccagcg ccttacgcgc
    31561 cgctttgatc acctgttcac actgttcaat ggtcagtgtc agtggcggtt caatgcggat
    31621 cgttttagcg ttgttgagtg ttccggccac caggacgcgc tggcggaaca tctcgctggc
    31681 aaagttatag ccgatttcgt tatcaacaaa ctcaatcgcc atcaacatcc ctttaccacg
    31741 cgcttcctgt accagatcgg gatattcccg cgccagttga cgaaaaccgt ccagcaacat
    31801 atcgcctttt tgttcagcct gagccggtaa gttctgctcc agcaacacat tgatggtcgc
    31861 cagcgccgcc gcacaggcca gcgggttgcc gccaaaggtg gtggtatgca ggaacgggtt
    31921 gtcgaacaga acggaaaaca cctcttcagt ggcgatggtt gcgccaatcg gcatcacgcc
    31981 gccgccgagg gcttttgcca gacacaggat gtccggctgt acgttctcat gctcgcaggc
    32041 gaacatcttg cccgtgcgcc ccataccggt ttgcacttca tcgaggatca tcagtgcgcc
    32101 gaactcatcg cacagcttac gcacggcggt gagatatccc ggcggcggca gaatgacgcc
    32161 accttcgccc tgaatcggtt cgaggatcac cgcagcaaca tcatcaccgg tttttttgca
    32221 ctcgttaaga gccgtgcgca tggcttcgat attgccaaac ggtacatgac gaaaccctgg
    32281 cagcagtggc ataaaaggtt tgcggaaggt cgatttcgcc gtggccgaca gcgcgccaag
    32341 tgatttaccg tggaacgcgc cgctggtggc aataaaagta aacttgccgc gcggtgactg
    32401 ataagccttc gccagcttca gcgccgcttc aacggattcg gtgccgctat tacagaagaa
    32461 gctgtatttc agtttaccgg gcgttagcgc cgcaagggtt ttcgccagca tcgcccgtaa
    32521 cggatcgagc agctcctggc tgtgaagcgg ttgtttagca agttgattct gtacggcgga
    32581 aaccacaact ggattacggt gccccacgtt gaaaattcca aaacctccca ggcagtcgat
    32641 aaactcctgt ccctgggtgt cgacaagcgt atttaaaccc cccgcttgcc actctacggc
    32701 tccgtaatcc ccgccagcgg taacagattt gcgatactct aaaaaccccg gattgacatg
    32761 ctctttgaag tattcaatca cctcccggtt aagtgctttc atctcctcat gatccagcgt
    32821 tcgcttctca atgagattca gggcgtgggc gctgcacgct aaagccgatg cgctcgaagg
    32881 taacctgttc aaaatatgct ccggaggctc gcgtatcaca tgataccgat ttaagtattg
    32941 cagggattgc gccactccgg ctgcgatcgc gaaaatcggg ataaacacaa atgtggttat
    33001 catttgcgca atatttaatg ctatttgtta acatctgttg ttattttgca cggtttttgt
    33061 cgggtatgga tttggcgatt gctgcgcaaa tgcacacttt cagggcaaca actgcctgat
    33121 ttttgggcaa g
//
LOCUS       DAZFLD010000024        32152 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.024, whole genome shotgun sequence.
ACCESSION   DAZFLD010000024 DAZFLD010000000
VERSION     DAZFLD010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32152)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32152)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32152
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.024"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(76..1122)
                     /gene="aroH"
                     /locus_tag="ACHKBL_001012"
     CDS             complement(76..1122)
                     /gene="aroH"
                     /locus_tag="ACHKBL_001012"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroH"
                     /protein_id="HFO5817924.1"
                     /translation="MNRTDELRTARIESLVTPAELALRYPVTPGVATHVTDSRRRIEK
                     ILNGEDKRLLVIIGPCSIHDLTAAMEYATRLQSLRNQYQSRLEIVMRTYFEKPRTVVG
                     WKGLISDPDLNGSYRVNHGLELARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGA
                     IGARTTESQIHREMASALSCPVGFKNGTDGNTRIAVDAIRAARASHMFLSPDKNGQMT
                     IYQTSGNPYGHIIMRGGKKPNYHADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRR
                     QLEVCEDICQQIRNGSTAIAGIMAESFLREGTQKIVGGQPLTYGQSITDPCLGWEDTE
                     RLVEKLASAVDTRF"
     gene            complement(1279..2112)
                     /gene="ppsR"
                     /locus_tag="ACHKBL_001013"
     CDS             complement(1279..2112)
                     /gene="ppsR"
                     /locus_tag="ACHKBL_001013"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HFO5817925.1"
                     /translation="MDNAVDRHVFYISDGTAITAEVLGHAVMSQFPVTISSITLPFVE
                     NESRARAVKDQIDAIYHQTGVRPLVFYSIVLPEIRAIILQSEGFCQDIVQALVAPLQQ
                     EMKLDPTPIAHRTHGLNPNNLNKYDARIAAIDYTLAHDDGISLRNLDQAQVILLGVSR
                     CGKTPTSLYLAMQFGIRAANYPFIADDMDNLVLPASLKPLQHKLFGLTIDPERLAAIR
                     EERRENSRYASLRQCRMEVAEVEALYRKNQIPWINSTNYSVEEIATKILDIMGLSRRM
                     Y"
     gene            2445..4823
                     /gene="ppsA"
                     /locus_tag="ACHKBL_001014"
     CDS             2445..4823
                     /gene="ppsA"
                     /locus_tag="ACHKBL_001014"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HFO5817926.1"
                     /translation="MSNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVS
                     VPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQP
                     ELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAV
                     KHVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQV
                     VFITSAWGLGEMVVQGAVNPDEFYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHG
                     KQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQ
                     ARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHDISEMNRIEPGDVL
                     VTDMTDPDWEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGENV
                     TVSCAEGDTGYVYAELLEFSVKSSSVETMPDLPLKVMMNVGNPDRAFDFACLPNEGVG
                     LARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLTEGIATL
                     GAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDCFAL
                     ECEAVKRVRNDMGLTNVEIMIPFVRTVDQAKAVVEELARQGLKRGENGLKIIMMCEIP
                     SNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSELFDERNDAVKALLSMAIRA
                     AKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK"
     gene            complement(4880..6526)
                     /gene="fadK"
                     /locus_tag="ACHKBL_001015"
     CDS             complement(4880..6526)
                     /gene="fadK"
                     /locus_tag="ACHKBL_001015"
                     /EC_number="6.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="medium-chain fatty-acid--CoA ligase"
                     /protein_id="HFO5817927.1"
                     /translation="MKVTLTFNEQRRAAYRQQGLWGDASLADYWQQTARAMPDKIAVV
                     DNHGATYTYSALDHAASCLANWMLAKGIESGDRIAFQLPGWCEFTVIYLACLKTGAVS
                     VPLLPSWREAELVWVLNKCQAKMFFAPTLFKQTRPVDLILPLQNQLPQLQQIVGVDKL
                     APATSALSLSQIIADNTPLTTAITVHGDELAAVLFTSGTEGLPKGVMLTHNNILASER
                     AYCARLNLTWQDVFMMPAPLGHATGFLHGVTAPFLIGARSVLLDIFTPAACLALLEQQ
                     RCTCMLGATPFVYDLLNLLEKQPADLSALRFFLCGGTTIPKKVARECQQRGIKLLSVY
                     GSTESSPHAVVNLDDPLSRFIHTDGYAAAGVEIKVVDDARKTLPPGCEGEEASRGPNV
                     FMGYFDEPELTARALDEEGWYYSGDLCRMDEAGYIKITGRKKDIIVRGGENISSREVE
                     DILLQHPKIHDACVVAMPDERLGERSCAYVVLKAPHHSLSLEDVVAFFSRKRVAKYKY
                     PEHIVVIEKLPRTASGKIQKFLLRKDIMRRLTQDVCEEIE"
     gene            complement(6583..6876)
                     /gene="ydiT"
                     /locus_tag="ACHKBL_001016"
     CDS             complement(6583..6876)
                     /gene="ydiT"
                     /locus_tag="ACHKBL_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HFO5817928.1"
                     /translation="MSQNATVNVDIKLGVNKFHVDEGHPHIILAANPDINEFRKLMKA
                     CPAGLYKQDDAGNIHFDSAGCLECGTCRVLCGNTILEQWQYPAGTFGIEFRYG"
     gene            complement(6873..8162)
                     /gene="ydiS"
                     /locus_tag="ACHKBL_001017"
     CDS             complement(6873..8162)
                     /gene="ydiS"
                     /locus_tag="ACHKBL_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFO5817929.1"
                     /translation="MSDDKFDAIVVGAGVAGSVAALVMARAGLDVLVIERGDSAGCKN
                     MTGGRLYAHTLDAIIPGFAASAPVERKVTREKISFLTEESAVTLDFHREQPDVPQHAS
                     YTVLRNRLDPWLMEQAEQAGAQFIPGVRVDALVREGNKVTGVQAGDDILEANVVILAD
                     GVNSMLGRSLGMVSASDPHHYAVGVKEVIGLTPEQINDRFNVTGEEGAAWLFAGSPSD
                     GLMGGGFLYTNKDSVSLGLVCGLGDIAHAQKSVPQMLEDFKQHPAIRPLISGGKLLEY
                     SAHMVPEGGLAMVPQLVNDGVMIVGDAAGFCLNLGFTVRGMDLAIASAQAAATTVIAA
                     KERADFSASSLAQYKRELEQSCVMRDMQHFRKIPALMENPRLFSQYPRMVADIMNEMF
                     TIDGKPNQPVRKMIMGHAKKIGLINLLKDGIKGATAL"
     gene            complement(8217..9155)
                     /gene="ydiR"
                     /locus_tag="ACHKBL_001018"
     CDS             complement(8217..9155)
                     /gene="ydiR"
                     /locus_tag="ACHKBL_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HFO5817930.1"
                     /translation="MSQLNSVWVFSDNPERYAELFGGAQQWGQQVYAIVQNTDQAQTV
                     MPYGPKCIYVLAQNDALQRTENYAESIAALLKDKHPSMLLLAATKRGKALAARLSVQL
                     NAALVNDATAVDIVDGHICAEHRMYGGLAFAQEKINSPLAIITLAPGVQEPCTSDTSN
                     QCPTETVPYVAPRHEILCRERRAKAASSVDLSKAKRVVGVGRGLAAQDDLKMVHELAA
                     VLNAEVGCSRPIAEGENWMERERYIGVSGVLLKSDLYLTLGISGQIQHMVGGNGAKVI
                     VAINKDKNAPIFNYADYGLVGDIYKVVPALISQLSR"
     gene            complement(9175..9939)
                     /gene="ydiQ"
                     /locus_tag="ACHKBL_001019"
     CDS             complement(9175..9939)
                     /gene="ydiQ"
                     /locus_tag="ACHKBL_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein"
                     /protein_id="HFO5817931.1"
                     /translation="MKIITCFKLVPEEQDIVVTPEYTLNFDNADAKISQFDLNAIEAA
                     SQLATDDDEIAALTVGGSLLQNSKVRKDVLSRGPHSLYMVQDAQLEHALPLDTAKALA
                     TAVEKIGFDLLIFGEGSGDLYAQQVGLLVGEILQLPVINAVSAIQRQGNTLVIERTLE
                     DDVEVIELSVPAVLCVTSDINVPRIPSMKAILGAGKKPVNQWQASDIGWSQSAPLAEL
                     TGIRVPPQTERKHIILDNDSPEAIAELAEHLKKALN"
     gene            10255..11166
                     /gene="ydiP"
                     /locus_tag="ACHKBL_001020"
     CDS             10255..11166
                     /gene="ydiP"
                     /locus_tag="ACHKBL_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO5817932.1"
                     /translation="MYQRCFDNASETLFVAGKTPRLSRFAFSDDPKWESGHHVHDNET
                     ELIYVKKGVARFTIDSSLYVAHADDIVVIERGRLHAVASDVNDPATTCTCALYGFQFQ
                     GAEENQLLQPHSCPVIAAGQGKEVIKTLFNELSVILPQSKNSQTSSLWDAFAYTLAIL
                     YYENFKNAYRSEQGYIKKDVLIKDILFYLNNNYREKITLEQLSKKFRASVSYICHEFT
                     KEYRISPINYVIQRRMTEAKWSLTNTELSQAEISWRVGYENVDHFAKLFLRHVGCSPS
                     DYRRQFKNCFAEQEILSEFPQPVSLAG"
     gene            complement(11209..12360)
                     /gene="ydiO"
                     /locus_tag="ACHKBL_001021"
     CDS             complement(11209..12360)
                     /gene="ydiO"
                     /locus_tag="ACHKBL_001021"
                     /EC_number="1.3.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HFO5817933.1"
                     /translation="MDFSLTEEQELLLASIRELITTNFPEEYFRTCDQNGTYPREFMR
                     ALADNGISMLGVPEEFGGIPADYVTQMLALMEVSKCGAPAFLITNGQCIHSMRRFGSA
                     EQLRKTAESTLETGDPAYALALTEPGAGSDNNSATTTYTRKNGKVYINGQKTFITGAK
                     EYPYMLVLARDPQPKDPKKAFTLWWVDSSKPGIKINPLHKIGWHMLSTCEVYLDNVEV
                     EESDMVGEEGMGFLNVMYNFEMERLINAARSTGFAECAFEDAARYANQRIAFGKPIGH
                     NQMIQEKLALMAIKIDNMRNMVLKVAWQADQHQSLRTSAALAKLYCARTAMEVIDDAI
                     QIMGGLGYTDEARVSRFWRDVRCERIGGGTDEIMIYVAGRQILKDYQNK"
     gene            complement(12374..13969)
                     /gene="ydiF"
                     /locus_tag="ACHKBL_001022"
     CDS             complement(12374..13969)
                     /gene="ydiF"
                     /locus_tag="ACHKBL_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl CoA:acetate/3-ketoacid CoA transferase"
                     /protein_id="HFO5817934.1"
                     /translation="MKPVKPPRINGRVPVLSAQEAVNYIPDEATLCVLGAGGGILEAT
                     TLITALADKYKQTQTPRNLSIISPTGLGDRADRGISPLAQEGLVKWALCGHWGQSPRI
                     SDLAEQNKIIAYNYPQGVLTQTLRAAAAHQPGIISDIGIGTFVDPRQQGGKLNEVTKE
                     DLIKLVEFDNKEYLYYKAIAPDIAFIRATTCDSEGYATFEDEVMYLDALVIAQAVHNN
                     GGIVMMQVQKMVKKATLHPKSVRIPGYLVDIVVVDPDQTQLYGGAPVNRFISGDFTLD
                     DSTKLSLPLNQRKLVARRALFEMRKGAVGNVGVGIADGIGLVAREEGCADDFILTVET
                     GPIGGITSQGIAFGANVNTRAILDMTSQFDFYHGGGLDVCYLSFAEVDQHGNVGVHKF
                     NGKIMGTGGFIDISATSKKIVFCGTLTAGSLKTEITDGKLNIVQEGRVKKFIRELPEI
                     TFSGKIALERGLDVRYITERAVFTLKEDGLHLIEIAPGVDLQKDILDKMDFTPVISPE
                     LKLMDERLFIDAAMGFVLPEAAH"
     gene            complement(14115..14873)
                     /gene="aroD"
                     /locus_tag="ACHKBL_001023"
     CDS             complement(14115..14873)
                     /gene="aroD"
                     /locus_tag="ACHKBL_001023"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HFO5817935.1"
                     /translation="MKTVTVKDLVIGTGAPKIIVSLMAKDIASVKSEALAYREADFDI
                     LEWRVDHYADLSNVESVIAAAKILRETMPEKPLLFTFRSAKEGGEQAISTEAYIALNR
                     AAIDSGLVDIIDLELFTGDDQVKETVAYAHAHDVKVVMSNHDFHKTPEAEEIIARLRK
                     MQSFDADIPKIALMPQSTSDVLTLLAATLEMQEQYADRPIITMSMAKTGVISRLAGEV
                     FGSAATFGAVKKASAPGQISVNDLRTVLTILHQA"
     gene            complement(14904..15770)
                     /gene="ydiB"
                     /locus_tag="ACHKBL_001024"
     CDS             complement(14904..15770)
                     /gene="ydiB"
                     /locus_tag="ACHKBL_001024"
                     /EC_number="1.1.1.282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
                     /protein_id="HFO5817936.1"
                     /translation="MDVTAKYELIGLMAYPIRHSLSPEMQNKALEKAGLPFTYMAFEV
                     DNDSFPGAIEGLKALKMRGTGVSMPNKQLACEYVDELTPAAKLVGAINTIVNDDGYLR
                     GYNTDGTGHIRAIKESGFDIKGKTMVLLGAGGASTAIGAQGAIEGLKEIKLFNRRDEF
                     FDKALAFAQRVNENTDCVVTVTDLADQQAFAEALASADILTNGTKVGMKPLENESLVN
                     DISLLHPGLLVTECVYNPHMTKLLQQAQQAGCKTIDGYGMLLWQGAEQFTLWTGKDFP
                     LEYVKQVMGFGA"
     gene            complement(15782..17047)
                     /gene="ydiN"
                     /locus_tag="ACHKBL_001025"
     CDS             complement(15782..17047)
                     /gene="ydiN"
                     /locus_tag="ACHKBL_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO5817937.1"
                     /translation="MSQNKAFSTPFILAVLCIYFSYFLHGISVITLAQNMTSLAEKFS
                     TDNAGIAYLISGIGLGRLISILFFGVISDKFGRRAVILMAVIMYLLFFFGIPACPNLT
                     LAYCLAVCVGIANSALDTGGYPALMECFPKASGSAVILVKAMVSFGQMFYPMLVSYML
                     LNNIWYGYGLIIPGILFVLITLMLLKSKFPSQLVDASVANELPQMNSKPLVWLEGVSS
                     VLFGVAAFATFYVIVVWMPKYAMAFAGMSEAEALKTISYYSMGSLVCVFIFAALLKKM
                     VRPIWANVFNSALATITAAIIYLYPSPLVCNAGAFVIGFSAAGGILQLGVSVMSEFFP
                     KSKAKVTSIYMMMGGLANFVIPLITGYLSNIGLQYIIVLDFTFALLALITAIIVFIRY
                     YRVFIIPENDVRFGEHKFSTRLNTIKHRG"
     gene            complement(17274..18488)
                     /gene="ydiM"
                     /locus_tag="ACHKBL_001026"
     CDS             complement(17274..18488)
                     /gene="ydiM"
                     /locus_tag="ACHKBL_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO5817938.1"
                     /translation="MKNPYYPTALGLYFNYLVHGMGVILMSLNMASLETLWQTNAAGV
                     SIVISSLGIGRLSVLLFAGLLSDRFGRRPFIMLGMCCYMAFFFGILHTNNIIIAYVFG
                     FLAGMANSFLDAGTYPSLMEAFPRSPGTANILIKAFVSSGQFLLPLIISLLVWAELWF
                     GWSFMIAAGIMFINALLLYRCTFPPHPGRHLPVIKKTTSSTEHRCSIIDLASYSLYGY
                     ISMATFYLVSQWLAQYGQFVAGMSYTMSIKLLSIYTVGSLLCVFITAPLIRNTVRPTT
                     LLMLYTFISFIALLTVCLHPTFYVVIIFAFVIGFTSAGGVVQIGLTLMAERFPYAKGK
                     ATGIYYSAGSIATFTIPLITAHLSQRSIADIMWFDTAIAAIGFILALFIGLRSRKETR
                     HHSLKENVAPGG"
     gene            complement(18588..18944)
                     /gene="ydiL"
                     /locus_tag="ACHKBL_001027"
     CDS             complement(18588..18944)
                     /gene="ydiL"
                     /locus_tag="ACHKBL_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiL family protein"
                     /protein_id="HFO5817939.1"
                     /translation="MNAYELQALRHIFAMTIDECATWIAQTDDSESWRQWENGKCAIP
                     DRVVEQLLAMRQQRKKHLHAIIEKINNRIGNNTMRFFPDLTVFQQVYPDGNFIDWKIY
                     QSVAAELYAHDLERLC"
     gene            complement(19080..19187)
                     /gene="rprA"
                     /locus_tag="ACHKBL_001028"
     ncRNA           complement(19080..19187)
                     /ncRNA_class="antisense_RNA"
                     /gene="rprA"
                     /locus_tag="ACHKBL_001028"
                     /product="antisense sRNA RprA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00034"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00034"
     gene            complement(19373..20485)
                     /gene="ydiK"
                     /locus_tag="ACHKBL_001029"
     CDS             complement(19373..20485)
                     /gene="ydiK"
                     /locus_tag="ACHKBL_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HFO5817940.1"
                     /translation="MVNVRQPRDVAQILLSVLFLAIMIVACLWIVQPFILGFAWAGTV
                     VIATWPVLLRLQKIMFGRRSLAVLVMTLLLVMVFIIPIALLVNSIVDGSGPLIKAISS
                     GDMTLPDLAWLNTIPVIGAKLYAGWHNLLDMGGTAIMAKVRPYIGTTTTWFVGQAAHI
                     GRFMVHCALMLLFSALLYWRGEQVAQGIRHFATRLAGVRGDAAVLLAAQAIRAVALGV
                     VVTALVQAVLGGIGLAVSGVPYATLLTVLMILSCLVQLGPLPVLIPAIIWLYWTGDTT
                     WGTVLLVWSGVVGTLDNVIRPMLIRMGADLPLILILSGVIGGLIAFGMIGLFIGPVLL
                     AVSWRLFAAWVEEVPPPTDQPEEILEELGEIEKSNK"
     gene            20872..23928
                     /gene="ydiJ"
                     /locus_tag="ACHKBL_001030"
     CDS             20872..23928
                     /gene="ydiJ"
                     /locus_tag="ACHKBL_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding and (Fe-S)-binding domain-containing
                     protein"
                     /protein_id="HFO5817941.1"
                     /translation="MIPQISQAPGVVQLVLNFLQELEQQGFTGDTATSYADRLTMSTD
                     NSIYQLLPDAVVFPRSTADVALIARLAAQERYSSLIFTPRGGGTGTNGQALNQGIIVD
                     MSRHMNRIIEINPEEGWVRVEAGVIKDQLNQYLKPFGYFFAPELSTSNRATLGGMINT
                     DASGQGSLVYGKTSDHVLGVRAVLLGGDILDTQPLPVELAETLGKSNTTIGRIYNTVY
                     QRCRQQRQLIIDNFPKLNRFLTGYDLRHVFNDEMTEFDLTRILTGSEGTLAFITEARL
                     DITRLPKVRRLVNVKYDSFDSALRNAPFMVEARALSVETVDSKVLNLAREDIVWHSVS
                     ELITDVPDKEMLGLNIVEFAGDDETLIDERVNALCARLDELIASQQAGVIGWQVCREL
                     AGVERIYAMRKKAVGLLGNAKGAAKPIPFAEDTCVPPEHLADYIAEFRALLDSHGLSY
                     GMFGHVDAGVLHVRPALDMCDPQQEILMKQISDDVVALTAKYGGLLWGEHGKGFRAEY
                     SPAFFGEELFAELRKVKAAFDPHNRLNPGKICPPEGLDAPMMKVDAVKRGTFDRQIPI
                     AVRQQWRGAMECNGNGLCFNFDARSSMCPSMKITQNRIHSPKGRATLVREWLRLLADR
                     GVDPLKLEQELPESGVSLRTLIARTRNSWHANKGEYDFSHEVKEAMSGCLACKACSTQ
                     CPIKIDVPEFRSRFLQLYHTRYLRPLRDHLVATVESYAPLMARAPKTFNFFINQPLVR
                     KLSEKHIGMVDLPLLSVPSLQQQMVGHRSANMTLEQLEALNAEQKARTVLVVQDPFTS
                     YYDAQVVADFVRLVEKLGFQPVLLPFSPNGKAQHIKGFLNRFAKTAKKTADFLNRMAK
                     LGMPMVGVDPALVLCYRDEYKLALGEERGEFNVLLANEWLASALDSQPVATVSGESWY
                     FFGHCTEVTALPGAPAQWVAIFARFGAKLENVSVGCCGMAGTYGHEAKNHKNSLGIYE
                     LSWHQAMQRLPRNRCLATGYSCRSQVKRVEGTGVRHPVQALLEIIK"
     gene            23925..24335
                     /gene="menI"
                     /locus_tag="ACHKBL_001031"
     CDS             23925..24335
                     /gene="menI"
                     /locus_tag="ACHKBL_001031"
                     /EC_number="3.1.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA hydrolase"
                     /protein_id="HFO5817942.1"
                     /translation="MIWKRKITLEALNAMGEGNMVGLLDIRFEHIGDDTLEATMPVDS
                     RTKQPFGLLHGGASVVLAESIGSVAGYLCTEGEQKVVGLEINANHVRSAREGRVRGVC
                     KPLHLGSRHQVWQIEIFDEKGRLCCSSRLTTAIL"
     gene            24435..24623
                     /gene="ydiH"
                     /locus_tag="ACHKBL_001032"
     CDS             24435..24623
                     /gene="ydiH"
                     /locus_tag="ACHKBL_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiH family protein"
                     /protein_id="HFO5817943.1"
                     /translation="MSTQLDPTQLAIEFLRRDQSNLSPAQYLKRLKQLELEFADLLTL
                     SSAELKEEIYFAWRLGVH"
     gene            25172..25540
                     /gene="sufA"
                     /locus_tag="ACHKBL_001033"
     CDS             25172..25540
                     /gene="sufA"
                     /locus_tag="ACHKBL_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HFO5817944.1"
                     /translation="MDMHSGTFNPQDFAWQGLTLTPAAAVHIRELVAKQPGMVGVRLG
                     VKQTGCAGFGYVLDSVSEPDKDDLLFEHDGAKLFVPLQAMPFIDGTEVDFVREGLNQI
                     FKFHNPKAQNECGCGESFGV"
     gene            25549..27036
                     /gene="sufB"
                     /locus_tag="ACHKBL_001034"
     CDS             25549..27036
                     /gene="sufB"
                     /locus_tag="ACHKBL_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HFO5817945.1"
                     /translation="MSRNTEATDDVKTWTGGPLNYKEGFFTQLATDELAKGINEEVVR
                     AISAKRNEPEWMLEFRLNAYRAWLEMEEPHWLKAHYDKLNYQDYSYYSAPSCGNCDDT
                     CASEPGAVQQTGANAFLSKEVEAAFEQLGVPVREGKEVAVDAIFDSVSVATTYREKLA
                     EQGIIFCSFGEAIHDHPELVRKYLGTVVPGNDNFFAALNAAVASDGTFIYVPKGVRCP
                     MELSTYFRINAEKTGQFERTILVADEDSYVSYIEGCSAPVRDSYQLHAAVVEVIIHKN
                     AEVKYSTVQNWFPGDNNTGGILNFVTKRALCEGENSKMSWTQSETGSAITWKYPSCIL
                     RGDNSIGEFYSVALTSGHQQADTGTKMIHIGKNTKSTIISKGISAGHSQNSYRGLVKI
                     MPTATNARNFTQCDSMLIGANCGAHTFPYVECRNNSAQLEHEATTSRIGEDQLFYCLQ
                     RGISEEDAISMIVNGFCKDVFSELPLEFAVEAQKLLAISLEHSVG"
     gene            27046..27792
                     /gene="sufC"
                     /locus_tag="ACHKBL_001035"
     CDS             27046..27792
                     /gene="sufC"
                     /locus_tag="ACHKBL_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HFO5817946.1"
                     /translation="MLSIKDLHVSVEDKAILRGLSLDVRPGEVHAIMGPNGSGKSTLS
                     ATLAGREDYEVTGGTVEFKGKDLLALSPEDRAGEGIFMAFQYPVEIPGVSNQFFLQTA
                     LNAVRSYRGQETLDRFDFQDLMEEKIALLKMPEDLLTRSVNVGFSGGEKKRNDILQMA
                     VLEPELCILDESDSGLDIDALKVVADGVNSLRDGKRSFIIVTHYQRILDYIKPDYVHV
                     LYQGRIVKSGDFTLVKQLEEQGYGWLTEQQ"
     gene            27767..29038
                     /gene="sufD"
                     /locus_tag="ACHKBL_001036"
     CDS             27767..29038
                     /gene="sufD"
                     /locus_tag="ACHKBL_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HFO5817947.1"
                     /translation="MAGLPNSSNALQQWHHLFEAEGAKRSPQAQQHLQQLLRTGLPTR
                     KHENWKYTPLDGLTNSQFVSIAGEISPQQRDALALTLDAVRLVFVDGRYVPALSDATE
                     GSGYEVSINDDRQGLPDAIQAEVFLHLTESLAQSVTHIAVKRGQRPAKPLLLMHITQG
                     VAGEEVNTAHYRHHLDLAEGAEATVIEHFVSLNDARHFTGARFTINVAANAHLQHIKL
                     AFENPLSHHFAHNDLLLADDATAFSHSFLLGGAVLRHNTSTQLNGENSTLRINSLAMP
                     VKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDKGRAVFNGLINVAQHAIKTDGQMTNNN
                     LLMGKLAEVDTKPQLEIYADDVKCSHGATVGRIDDEQMFYLRSRGINQQDAQQMIIYA
                     FAAELTEALRDEGLKQQVLARIGQRLPGGAR"
     gene            29035..30255
                     /gene="sufS"
                     /locus_tag="ACHKBL_001037"
     CDS             29035..30255
                     /gene="sufS"
                     /locus_tag="ACHKBL_001037"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase SufS"
                     /protein_id="HFO5817948.1"
                     /translation="MTFSVDKVRADFPVLSREVNGLPLAYLDSAASAQKPSQVIDAEA
                     EFYRHGYAAVHRGIHTLSAQATEKMENVRKRASLFINARSAEELVFVRGTTEGINLVA
                     NSWGNSNVRAGDNIIISQMEHHANIVPWQMLCARVGAELRVIPLNPDGTLQLETLPTL
                     FDEKTRLLAITHVSNVLGTENPLAEMITLAHQHGAKVLVDGAQAVMHHPVDVQALDCD
                     FYVFSGHKLYGPTGIGILYVKEALLQEMPPWEGGGSMIATVSLSEGTTWTKAPWRFEA
                     GTPNTGGIIGLGAALEYVSALGLNNIAEYEQNLMHYALSQLESVPDLTLYGPQNRLGV
                     IAFNLGKHHAYDVGSFLDNYGIAVRTGHHCAMPLMAYYNVPAMCRASLAMYNTHEEVD
                     RLVTGLQRIHRLLG"
     gene            30268..30684
                     /gene="sufE"
                     /locus_tag="ACHKBL_001038"
     CDS             30268..30684
                     /gene="sufE"
                     /locus_tag="ACHKBL_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfuration protein SufE"
                     /protein_id="HFO5817949.1"
                     /translation="MALLPDKEKLLRNFLRCANWEEKYLYIIELGQRLPELRDEDKSP
                     QNSIQGCQSQVWIVMRQNAQGIIELQGDSDAAIVKGLIAVVFILYDQMTPQDIVNFDV
                     RPWFEKMALTQHLTPSRSQGLEAMIRAIRAKAAALS"
     gene            30833..31837
                     /gene="ldtE"
                     /locus_tag="ACHKBL_001039"
     CDS             30833..31837
                     /gene="ldtE"
                     /locus_tag="ACHKBL_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtE"
                     /protein_id="HFO5817950.1"
                     /translation="MKCASLLTLTLIGAFSAIQAAWAVDYPLPPTGSRLVGQNQTYTV
                     QEGDKNLQAIARRFDTAAMLILEANNTIAPVPKPGTTITIPSQLLLPDAPRQGIIVNL
                     AELRLYNYPPGENIVQVYPIGIGLQGLETPVMETRVGQKIPNPTWTPTAGIRQRSLER
                     GIKLPPVVPAGPNNPLGRYALRLAHGNGEYLIHGTSAPDSVGLRVSSGCIRMNAPDIK
                     ALFSSVRTGTPVKVINEPVKYSVEPNGMRYVEVHRPLSAEEQQNVQTMPYTLPAGFTQ
                     FKDNKGVDQKLVDKALYRRAGYPVAVSSGATPAASNAPSVESAQNGEPEQGNMLRATQ
                     "
     gene            complement(31901..32137)
                     /gene="lpp"
                     /locus_tag="ACHKBL_001040"
     CDS             complement(31901..32137)
                     /gene="lpp"
                     /locus_tag="ACHKBL_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein lipoprotein Lpp"
                     /protein_id="HFO5817951.1"
                     /translation="MKATKLVLGAVILGSTLLAGCSSNAKIDQLSSDVQTLNAKVDQL
                     SNDVNAMRSDVQAAKDDAARANQRLDNMATKYRK"
BASE COUNT         8154 a         7939 c         8191 g         7868 t
ORIGIN      
        1 cagagcaagc agtaatttgt tgagaagaca acaagttgcg cagaaatgcc catcccgtca
       61 ggaatgggca cgcattcaga agcgagtatc taccgcagag gcgagttttt cgaccaggcg
      121 ttcggtatct tcccagccca gacacgggtc ggtaatggat tgaccgtaag tgagcggctg
      181 accgccgacg attttttgcg ttccttcgcg caggaaactt tccgccataa ttccagcaat
      241 cgccgtagat ccattgcgga tttgctgaca aatatcctca caaacttcta actggcgtcg
      301 gtgctgcttc tggcagttac cgtggctgaa atccaccacc agatgttcag gtaaatcaaa
      361 ctcgtgcagc gtatcgcagg ctgcggcgat atcatcggca tgataattcg gttttttgcc
      421 gccacgcata ataatgtggc catacgggtt gccgctggtc tgatagatgg tcatctgacc
      481 atttttgtct ggcgagagga acatatggct ggcgcgggct gcgcggatag catccacggc
      541 aatccgcgtg ttgccatcgg taccattttt aaaacctacc ggacaggaga gtgccgaagc
      601 catttcacgg tggatctgac tttcggtagt acgtgcgcca atcgcgcccc aactgattaa
      661 atcagcaata aactgaccgg tcaccatatc gaggaactca gtcgcggtag ggacgcccag
      721 ctcatttacc tgtaaaagta atttgcgcgc cagctccaga ccgtgattta cccgatagct
      781 gccgtttaaa tctggatcgg agattagccc tttccagccg acaacagttc gtggtttttc
      841 aaaataggtg cgcattacga tttccagccg tgactggtac tggttgcgca gcgactgcag
      901 acgggtggcg tactccattg cagcggtgag atcgtggatc gagcaggggc caataatgac
      961 caacagtcgc ttatcttcac cattcagtat tttttcaatt ctgcggcggg agtcggtgac
     1021 atgggtggcg acgccaggcg ttacgggata ccgtagcgcg agttcggcgg gcgttaccag
     1081 gctctcaata cgcgcagtac ggagttcgtc agttctgttc attacaagtc tcagaaattt
     1141 gttgcttcta cggcaggact accgggaagt gatgcgcatc acgataaacc aatcgcagct
     1201 gatttcaagt gcgaggtgtg tcacacctcg ccgggtggtt agcatgataa caaaaaaaca
     1261 agctaatgca ctagttctct agtacattcg acggctaagg cccatgatat cgaggatctt
     1321 ggtggcaatc tcttctaccg aataattggt actgttaatc cacgggatct gatttttacg
     1381 gtacaacgct tccacttccg cgacttccat cctgcactga cgaagcgagg catagcgact
     1441 gttctcccga cgttcctcgc gaatcgccgc cagacgttcc gggtcgatag tcaggccgaa
     1501 caatttatgc tgaagcggtt tgagcgacgc gggtagcaca agattatcca tatcgtcggc
     1561 aataaagggg tagtttgccg cgcggatacc aaactgcatt gccagataca gactggtggg
     1621 ggttttacca cagcgagaaa cgccgagcag gatcacctga gcctggtcca gattgcgtaa
     1681 cgaaatgcca tcatcgtggg cgagggtgta atcaatcgca gcaatgcgcg catcatattt
     1741 attgagatta ttagggttaa ggccatgggt acgatgagca atcggcgttg gatccagttt
     1801 catctcttgt tgtagcgggg caaccagcgc ctgaacgata tcctggcaaa agccctcact
     1861 ttgcaagatg atggcgcgaa tctccggcaa cacgatggag tagaagacca gcgggcgcac
     1921 acctgtctgg tgataaattg cgtcaatctg atccttcact gcccgtgcac ggctctcatt
     1981 ttcgacaaac ggcagcgtaa tgctgctgat agtgacggga aattgtgaca ttactgcgtg
     2041 tcctaatacc tccgcagtta ttgccgtacc atcagaaata taaaaaacgt ggcgatcaac
     2101 agcattatcc attttgttct tcccgtgatg cagacataat tctatgaaag cataaattaa
     2161 aaacgcacag aagcgtagaa cgttatgtct ggtttataaa atgaaccttc aattttattt
     2221 tttatgaaaa cagcatttca tttttacggt ttcgtttata ccgatggttt atgtggaaat
     2281 tgtcgaagag agcagatttg cgcaacgctg ggatcagtct taaaaagtaa aaaaatatat
     2341 ttgcttgaac gattcaccgt ttttttcatc cggttaaata tgcaaagata aatgcgcaga
     2401 aatgtgtttc tcaaaccgtt catttatcac aaaaggattg ttcgatgtcc aacaatggct
     2461 cgtcaccgct ggtgctttgg tataaccaac tcggcatgaa tgatgtagac agggttgggg
     2521 gcaaaaatgc ctccctgggt gaaatgatta ctaacctttc cggaatgggt gtttccgttc
     2581 cgaatggttt cgccacaacc gccgacgcgt ttaaccagtt tctggaccaa agcggcgtaa
     2641 accagcgcat ttatgaactg ctggataaaa cggatattga cgatgttacc cagcttgcga
     2701 aagcgggcgc gcaaatccgc cagtggatta tcgacactcc cttccagcct gagctggaaa
     2761 acgccatccg cgaagcctat gcacagcttt ccgccgatga cgaaaacgcc tcgtttgcgg
     2821 tgcgttcctc cgctactgca gaagatatgc cggacgcttc ttttgccggt cagcaggaaa
     2881 ctttcctcaa cgttcagggt tttgacgccg ttctcgtggc tgtgaaacat gtatttgctt
     2941 ctctgtttaa cgatcgcgcc atctcttatc gtgtgcacca gggttacgat caccgtggcg
     3001 tggcgctgtc cgccggtgtt cagcggatgg tgcgctccga cctcgcatct tctggcgtga
     3061 tgttctccat tgataccgaa tccggctttg accaggtggt gtttatcact tccgcatggg
     3121 gccttggtga aatggtcgtg cagggtgcgg ttaacccgga tgagttttac gtgcacaaac
     3181 cgacactggc ggcgaatcgc ccggctatcg tgcgccgcac catggggtcg aaaaaaatcc
     3241 gcatggttta cgcgccgacc caggagcacg gcaagcaggt taaaatcgag gacgtaccgc
     3301 aagaacagcg tgacatcttc tcgctgacca acgaagaagt gcaggaactg gcaaaacagg
     3361 ccgtacaaat tgagaaacac tacggtcgcc caatggatat tgagtgggcg aaagatggcc
     3421 acaccggtaa actgttcatt gtgcaggcgc gtccggaaac cgtgcgctca cgcggtcagg
     3481 tcatggagcg ttatacgctg cattcacagg gtaagattat cgccgaaggc cgtgctatcg
     3541 gtcatcgcat cggtgcgggt ccggtgaaag tcatccatga catcagcgaa atgaaccgca
     3601 tcgaacctgg agacgtgctg gtcactgaca tgaccgaccc ggactgggaa ccgatcatga
     3661 agaaagcatc tgccatcgtc accaaccgtg gcggtcgtac ctgtcacgcg gcgatcatcg
     3721 ctcgtgaact cggcattccg gcggtagtgg gctgtggtga tgcaacagaa cggatgaaag
     3781 acggtgagaa cgtcactgtt tcttgtgccg aaggtgatac cggttacgtc tatgcggagt
     3841 tgctggaatt tagcgtgaaa agctccagcg tagaaacgat gcctgatctg ccgttgaagg
     3901 tgatgatgaa cgtcggtaac ccggaccgtg ctttcgactt cgcctgcctg ccgaacgaag
     3961 gcgtcggcct tgcgcgtctg gaatttatca tcaaccgtat gattggcgtc cacccacgcg
     4021 cactgcttga gtttgacgat caggaaccgc agttgcaaaa cgaaatccgc gagatgatga
     4081 aaggttttga ttctccgcgt gaattttacg ttgggcgtct gactgaaggg atcgcgacgc
     4141 tgggtgccgc gttttatccg aagcgcgtca ttgtccgtct ctctgatttt aaatcgaacg
     4201 aatatgccaa cctggtcggt ggtgagcgtt acgagccaga tgaagagaac ccgatgctcg
     4261 gcttccgtgg cgcgggccgc tatgtttccg acagcttccg cgactgcttc gcgctggagt
     4321 gtgaagcagt gaaacgtgtg cgcaacgaca tggggctgac caacgttgag atcatgatcc
     4381 cgttcgtgcg taccgtagat caggcgaaag cggtggttga ggaactggcg cgtcaggggc
     4441 tgaaacgtgg tgagaacggg ctgaaaatca tcatgatgtg tgaaattccg tccaacgcct
     4501 tgctggcaga gcagttcctc gaatatttcg acggcttctc aattggctca aacgacatga
     4561 cacagctggc gctcggtctg gaccgtgact ccggagtggt gtctgaactg ttcgatgagc
     4621 gcaacgatgc ggtgaaagca ctgctgtcga tggcgattcg tgccgcgaag aaacagggca
     4681 aatatgtcgg gatttgcggt cagggtccgt ccgaccacga agactttgcc gcatggttga
     4741 tggaagaggg gatcgatagc ctgtctctga acccggatac cgtggtgcaa acctggttaa
     4801 gcctggctga actgaagaaa taaaataaat ccccggcggc gttttgtcgc cgggtttatg
     4861 tgatccccga agatgaaact tattcaatct cttcacagac atcctgagtt aatcgccgca
     4921 taatatcttt ccttaacaaa aacttttgta ttttacctga ggctgtgcgc ggtagttttt
     4981 cgattaccac gatatgttca ggatatttat atttcgcgac ccgtttacgg ctaaaaaatg
     5041 ccactacatc ttccagcgat aatgaatgat gcggtgcttt cagcacgaca taagcgcatg
     5101 aacgttcacc taagcgttca tcgggcattg caaccacaca agcatcgtga attttaggat
     5161 gctgcaataa aatatcttct acttcacggc tgctaatatt ttcgccgccg cggacaataa
     5221 tatctttctt gcgcccggtt atttttatat agccagcctc atccatgcgg cagagatcgc
     5281 cgctgtaata ccagccttct tcatccagcg cacgggcggt taattcaggt tcatcaaaat
     5341 accccataaa cacattgggg ccacgcgagg cttcttcacc ttcgcaacct ggcggtaaag
     5401 tcttgcgtgc gtcatcgacc actttaatct ctacacctgc ggcagcgtaa ccatcggtgt
     5461 gtataaagcg cgacaaagga tcatcgagat tcaccaccgc atgcggcgaa ctttctgtgg
     5521 aaccataaac acttaataat ttaatgccgc gctgctggca ttcacgcgcc acttttttgg
     5581 ggattgtggt accgccgcaa agaaagaaac gcagcgctga aaggtctgcg ggctgtttct
     5641 ccagtaaatt caaaagatca tagacaaacg gcgttgcgcc gagcatacag gtgcaacgct
     5701 gctgctcaag cagcgcgaga cacgcagcag gagtgaaaat atctaacaac acgctgcgag
     5761 caccaatcaa aaaaggtgct gttacgccat gcagaaagcc cgttgcgtga ccaagtggcg
     5821 caggcatcat aaagacatcc tgccaggtca gatttagccg cgcgcaataa gcccgctcac
     5881 tggcgagaat attgttatgc gttagcatca cgccctttgg cagaccctcg gttccggagg
     5941 taaacagcac cgcggctaat tcatcgccgt ggaccgttat cgccgtggtc agaggagtat
     6001 tgtcggcgat aatctgactt aatgagagag cagaggtggc gggagccagt ttgtccacgc
     6061 cgacaatttg ttgtagttgt ggaagctgat tttgcagcgg caggattaaa tctaccggac
     6121 gcgtttgttt aaacaacgtc ggtgcaaaga acatttttgc ctgacactta ttgagcaccc
     6181 acaccagttc tgcttctcgc caggaaggca acagcggcac ggaaaccgca ccggttttca
     6241 ggcaggcaag atagataacg gtaaattcac accagcctgg cagttgaaat gcgatgcgat
     6301 cgcctgattc aatacccttc gctaacatcc agtttgccag acagctcgcg gcgtgatcga
     6361 gcgcgctata agtgtacgtt gcaccatgat tgtcgaccac ggcaattttg tctggcatcg
     6421 cacgagcggt ctgctgccag taatcggcca gtgaagcatc gccccataac ccttgctgac
     6481 gatacgccgc acgacgttgt tcgttgaacg ttaatgtcac tttcattttg gactctcgaa
     6541 acagaacatc aggcccgaga tgcgggcctg tgggatgcat gattagccgt agcgaaattc
     6601 aataccgaag gtgcctgcgg gatattgcca ctgttcgaga atagtgttgc cgcacagcac
     6661 ccgacaggtg ccgcactcca gacaaccggc ggaatcgaaa tgaatgtttc ctgcgtcatc
     6721 ctgcttataa agtccggcag ggcaggcttt cattaattta cggaattcat tgatatcggg
     6781 atttgccgcc agaatgatat gcgggtggcc ctcatcaaca tggaatttat ttacgcctaa
     6841 tttgatgtcg acgttaaccg tagcgttctg gctcatagcg cggttgctcc cttaatgcca
     6901 tctttcagca agttgatcag tccaattttc ttcgcgtgtc ccatgatcat tttgcgtacc
     6961 ggttggttag gtttgccgtc gatggtgaac atctcgttca tgatgtcggc gaccattcgt
     7021 gggtattggc taaacaggcg cgggttttcc atcagcgccg ggatcttgcg aaaatgctgc
     7081 atatcacgca tgacgcagct ttgttccagc tcacgtttgt attgcgcaag actgctggcg
     7141 gagaaatccg cgcgttcttt ggcggcgatc actgttgtgg cggcagcctg agccgatgca
     7201 atggctaaat ccatgccgcg gaccgtaaaa cccaaattca ggcagaagcc tgcggcgtca
     7261 ccaacgatca tcacgccatc gttaaccagc tgcggcacca ttgccagacc gccctccggc
     7321 accatatgcg cggagtattc aagcagtttg ccgccgctaa tcagcgggcg aatggcgggg
     7381 tgttgtttaa aatcttccag catttgcggc acactttttt gcgcatgggc gatatcaccc
     7441 aatccacaaa ccagccccaa cgatacggaa tctttgttgg tatagaggaa tccgccgccc
     7501 atcaggccgt cagaagggga accggcaaac agccaggcgg caccttcctc gcccgtaaca
     7561 ttaaagcgat cgttgatctg ttctggtgtc aggcctataa cctctttaac accaacagcg
     7621 taatgatgcg gatcggaagc ggaaaccatt cccagcgagc ggccaagcat cgagttaacg
     7681 ccatcagcca gaatcaccac attcgcttcg agaatatcat ccccggcctg gacgccagtg
     7741 accttgtttc cttcacgaac cagtgcatcg acgcgtacgc ccgggataaa ctgcgcgccc
     7801 gcctgctcgg cttgttccat caaccacggg tccagacgat tacgcaatac ggtataagac
     7861 gcgtgttgcg gaacatctgg ttgctcgcgg tgaaaatcga gggtaacggc gctttcttcg
     7921 gttaagaagg agattttctc gcgtgtgacc ttgcgttcta ccggcgctga tgctgcaaag
     7981 cctggaataa ttgcgtcaag tgtgtgggca taaagacgcc cgccggtcat gtttttacat
     8041 ccggcactgt cgccgcgttc tatcaccagg acatccagcc cggctcgcgc catgaccagt
     8101 gcggcaacgc taccggcaac gcccgcaccg accacaatgg catcaaattt gtcatccgac
     8161 atgcgttatg ctcccttccc gctgcgttta cagcgggaga tgtggttgaa agggaatcag
     8221 cgactcaact ggctaatcag ggcagggacg actttgtaga tatcgcccac cagaccgtag
     8281 tcggcatagt tgaagattgg cgcattttta tctttattaa tggcgacaat cacttttgcg
     8341 ccgttgccgc caaccatatg ctggatctgc ccggagatcc ccagcgtcag gtagagatcg
     8401 gatttcagca acacgccgga gacaccgata taacgttcac gctccatcca gttctcgcct
     8461 tcggcaattg gacgtgaaca gccgacttca gcattcagca ccgccgccag ttcgtggacc
     8521 attttcaggt catcctgcgc tgccagacca cgaccgacgc caaccacacg ttttgctttg
     8581 ctcaggtcca cgctgcttgc cgctttcgca cggcgttcgc gacagagaat ttcatgacgc
     8641 ggggcaacat aaggtaccgt ttctgtcgga cactgattag aggtatcact ggtgcacggt
     8701 tcctgaacac cgggcgcaag ggtaatgatc gccagcgggc tattgatctt ttcctgtgcg
     8761 aacgctaacc cgccatacat ccggtgttcg gcgcaaatgt gaccatcgac aatatccacc
     8821 gcagtggcat cgttcaccag cgccgcattc agttgcacac ttaaccgtgc agccagagct
     8881 ttaccgcgtt tcgtcgcggc cagcagcaac atagaagggt gtttatcttt cagcagggca
     8941 gcaatgcttt cggcgtaatt ttcagtacgt tgcagcgcgt cgttttgcgc aagaacataa
     9001 atgcattttg gcccataagg cataactgtc tgcgcctggt cggtattttg tacaatggca
     9061 tacacctgtt ggccccattg ctgagcgccg ccaaacagtt cagcataacg ttcaggatta
     9121 tcgctaaaga cccagacgct gtttaattga ctcatcgttt cttctccgta ggcttcagtt
     9181 cagggctttc ttcagatgtt ccgccagctc ggcaatggcc tccggcgaat cgttatcgag
     9241 aatgatgtgc ttacgttctg tttgcggcgg tacgcgaatg ccggtcagtt cagcaagtgg
     9301 tgcgctctgg ctccagccaa tatcacttgc ctgccactga tttaccggtt ttttacccgc
     9361 gccgaggatg gctttcatcg aaggaatacg tggtacgtta atatcggagg tgacgcagag
     9421 cacggctgga acagagagtt caataacttc aacatcatct tcaagcgtgc gttcaatcac
     9481 cagtgtattg ccctgacgct gaatggcact cactgcatta atcaccggaa gttgcagaat
     9541 ttctccgacc agcaagccaa cctgctgggc ataaaggtcg ccggaacctt caccaaagat
     9601 cagtaaatcg aagccgatct tttcaactgc tgtcgccagc gcctttgcgg tatcgagagg
     9661 cagtgcatgc tcaagttgtg catcctgcac catatacagg ctgtgcggcc cgcgggatag
     9721 cacgtctttg cgcactttcg agttctgcaa caaagagccg ccgacggtca gcgcggctat
     9781 ctcatcgtca tctgttgcaa gctggcttgc agcttcaatg gcattgagat cgaactggct
     9841 gattttggcg tcggcattgt cgaaattcag ggtatattct ggagtgacaa caatgtcctg
     9901 ttcttcaggc accagcttaa agcaggttat tattttcatg atttctcctg tgagtcgcat
     9961 acggaaccat ctccgatgga aggtaaaaat ggcctgaaaa tagtgtgcga tagcggtgcg
    10021 gggttttcga cggagcaagc tgttttcctt gccaacaatc ttgcaaaaaa aatgcgatcg
    10081 ctaaaacaaa agtgcaattt gcggcaatgt tgttattcca tgaccgtaca atcttgcatt
    10141 cagtgtaaat aatcttgttt agctctcgct aaacgcggtt gtataaccgc aatacacgcc
    10201 gtattttatc cggcatatat tgtttttgct tgctcatgga atagggaggt tgtcatgtat
    10261 caacgctgtt ttgataacgc cagcgaaacg ctgtttgtcg ccggtaaaac gccacggctt
    10321 tcacgttttg catttagcga tgatccaaaa tgggagtccg gacatcacgt tcatgacaat
    10381 gaaaccgagt tgatttacgt caagaaaggg gtagcaaggt ttaccatcga ttcttcgtta
    10441 tatgtcgcgc atgccgatga cattgtggtg atagaacgtg gcaggttgca tgcggtggcc
    10501 tctgacgtta acgatccagc aacgacgtgt acctgtgcgc tgtacggctt tcagtttcag
    10561 ggggctgagg aaaatcagct actgcaaccg cattcttgcc cggtaattgc cgcagggcag
    10621 ggaaaagaag tcattaaaac cttatttaat gagctaagtg tgattttgcc gcaaagtaaa
    10681 aatagccaaa catcttcgtt atgggacgca tttgcctata cgttagcaat tctttactat
    10741 gaaaacttta aaaatgctta tcgttcggag caggggtata ttaaaaaaga tgttctgata
    10801 aaagatattc ttttctatct gaataataat tatcgcgaaa aaatcacttt agagcagtta
    10861 tcgaaaaagt ttcgtgccag cgtcagttat atttgccatg aatttaccaa agagtatcgc
    10921 atttccccta ttaactatgt tattcaacgg cgtatgacgg aagcgaaatg gtcactcact
    10981 aatactgaat tatcacaggc agagatctcc tggcgtgtgg gttatgaaaa tgtcgatcac
    11041 tttgccaaac tgtttttgcg ccatgtcggc tgttcgccca gcgattaccg caggcaattt
    11101 aaaaactgtt ttgcggaaca agaaatccta tctgaatttc ctcaaccagt aagtcttgcc
    11161 ggataaatct ggcggcgcag tttgttaaga agctgcgccg cctgcagatt atttgttctg
    11221 ataatctttc aggatctgcc gacctgctac gtaaatcata atttcgtctg taccgccgcc
    11281 gatacgttca caacggacat cacgccagaa gcgggagacg cgcgcctcat cggtatagcc
    11341 cagaccgccc atgatttgaa tcgcatcatc aatgacttcc attgcggtac gtgcgcagta
    11401 cagttttgcc agcgccgcgc tggtgcgcag tgactgatgc tgatcggctt gccatgccac
    11461 tttcaacacc atgttgcgca tgttgtcgat cttaatcgcc atcagcgcca gtttttcctg
    11521 gatcatctgg ttatgaccaa tgggcttacc aaaagcgata cgctggttgg catagcgggc
    11581 ggcatcttcg aatgcgcatt cggcaaagcc ggtgctgcgc gcggcgttga tcaggcgctc
    11641 catctcaaag ttgtacatca cattgaggaa acccattcct tcttcgccca ccatgtcgct
    11701 ctcttcaact tccacgttgt cgagatagac ttcgcaggtg ctgagcatat gccagccgat
    11761 tttatgcagt ggattgattt taatgccggg cttactggag tcgacccacc acagagtaaa
    11821 ggcctttttc ggatctttcg gttgcggatc gcgcgccaac accagcatat atggatactc
    11881 tttcgcaccg gtaataaagg ttttctgtcc gttgatgtaa accttgccgt ttttacgcgt
    11941 gtaagtggta gtggcactgt tgttgtctga gcctgcgcct ggctctgtca atgccagggc
    12001 ataggcggga tcacccgttt ccagggtgct ttccgccgtt ttacgtagct gctctgcaga
    12061 accgaaacgg cgcatactgt gaatacattg accgttggtg atcaaaaatg ccggggcacc
    12121 gcattttgac acttccatca gcgccagcat ttgggtgacg taatccgcag ggataccacc
    12181 aaattcttcc ggcacgccaa gcatggaaat accgttatcc gccagtgccc gcataaattc
    12241 acgcgggtat gtcccgtttt gatcgcaggt gcggaaatac tcttccggaa agttagtcgt
    12301 aatcagttcg cgaatactgg ccagcagcag ttcttgttct tcagttaaag aaaaatccat
    12361 cattatactc ctgttaatga gccgcttcag gcaggacaaa acccatcgcc gcatcgataa
    12421 ataatctttc gtccatcagt ttgagttctg gcgaaatcac tggggtgaaa tccattttgt
    12481 cgagaatatc tttttgtaaa tcgacgccag gagcgatttc gattaaatgc aggccgtctt
    12541 ctttcagcgt gaatacggcg cgctcagtga tataacgaac atccagccct cgctcgagcg
    12601 cgatttttcc gctaaaagta atttctggta gttcccgaat aaatttcttc acccgtcctt
    12661 cctggacgat atttaatttg ccgtcggtaa tttctgtttt taaactgccc gcagttaatg
    12721 tgccgcagaa aacgattttc ttcgaagtgg cactgatatc aataaatcca ccggtgccca
    12781 tgattttacc attgaattta tgcacgccga cgttaccgtg ctggtcgact tcagcaaaac
    12841 tcaaataaca aacatccaga ccaccaccgt gataaaaatc aaactgggac gtcatatcca
    12901 gaatggcgcg ggtattcacg ttcgcgccaa aggcgatccc ctgtgaagta ataccgccaa
    12961 tcggacctgt ttctaccgtc agaataaagt catcagcaca accttcttcc cgggcgacca
    13021 ggccaatgcc gtcagcaata ccgacgccga cattccccac tgcgcctttg cgcatttcga
    13081 ataatgcacg ccgtgcaact aatttacgtt ggtttagggg caggctaagt ttggtgctgt
    13141 catcaagggt gaagtcacca gaaataaagc ggttaaccgg cgctccgcca tacagttggg
    13201 tttgatccgg atcgaccacc acaatatcca ccagataacc cggaatacgg acagatttag
    13261 gatgcagcgt ggctttctta accattttct gcacctgcat catcacaata ccgccgttat
    13321 tgtgcaccgc ctgagcaata accaatgcgt cgagatacat cacctcatct tcaaaagtgg
    13381 cgtagccttc gctgtcgcag gtggtagcgc gaatgaaggc gatatctggc gctatcgctt
    13441 tgtaatagag atattctttg ttatcaaact cgaccagttt aatcaggtct tctttagtga
    13501 cttcattcag tttgccgcct tgctggcgtg gatcgacaaa tgttccgatg ccaatatcac
    13561 taataatacc aggctggtgg gctgcggcgg cgcgtaaggt ttgtgtaagt acaccttgtg
    13621 gatagttata agcaataatt ttattttgtt ctgcgagatc agaaatacgc ggcgattgtc
    13681 cccagtgacc gcataatgcc catttcacca ggccttcttg cgccagcgga ctaataccac
    13741 ggtcggcgcg atcgccaagc cctgttggac taataatcga taaattacgt ggtgtttgag
    13801 tctgtttata tttatcagca agagcagtaa ttaacgtggt ggcttccaga ataccgccgc
    13861 cagcgcctaa cacacaaagt gttgcttcgt cgggaatata attcaccgct tcctgtgccg
    13921 acaggaccgg cactcgtcca ttaatgcgag gtggttttac aggtttcatt gttaattcct
    13981 gattcaggtg tgattttccc tacgccagat ggagaattag cgcacagaga ctcacgaaat
    14041 atttttatta aatacggtta aaaatacttt ttttagttcg gcgggggaga gtattcccgc
    14101 cgaaatatta ttgcttatgc ctgatgtaaa atagttaata ccgtacgcaa atcatttacc
    14161 gagatttgcc ctggcgcaga ggcttttttt accgcaccaa aagttgccgc cgagccaaat
    14221 acttcaccag ccagacgaga aattacgcca gtttttgcca tcgacatcgt gatgattgga
    14281 cgatcggcat actgctcctg catctccagg gtcgcagcaa gcaacgtcag cacatcgctg
    14341 gtactttgcg gcatcagcgc aatcttagga atatcggcat cgaaggactg cattttgcgc
    14401 agacgggcaa tgatttcttc ggcttccggc gttttatgga agtcatggtt ggacatgaca
    14461 actttcacat catgcgcgtg ggcgtaggcg acggtttctt taacctgatc atcgccggta
    14521 aataactcca gatcgattat atcaaccagg ccgctgtcga tggctgcacg attgagtgca
    14581 atataagcct cggtggaaat cgcctgctcg ccgccttctt tggcactgcg gaaggtaaac
    14641 agcagcggtt tttctggcat ggtttcacgg agaatttttg ctgccgctat gacagactcc
    14701 acattggaga gatcggcata gtggtccaca cgccattcca gaatatcaaa gtccgcttca
    14761 cgataggcga gagcttcgga tttcacgctg gcgatatctt tcgccatcag cgagacgatg
    14821 attttaggtg cgcccgtacc aatgacgaga tcttttacag ttacggtttt cattttttac
    14881 cctttctgca cgcggtcagc ctgtcaggca ccgaacccca tgacctgttt aacatattcc
    14941 agagggaaat ctttgccagt ccataacgtg aactgttcag ccccttgcca caacaacatg
    15001 ccgtatccat caatcgtttt gcaacccgct tgttgcgcct gctgcaataa cttcgtcata
    15061 tgagggttat acacgcattc agtgaccaga agtcccggat gtaacagact gatatcatta
    15121 accaatgatt cattctcaag gggtttcata cccactttgg tgccattggt taaaatgtcg
    15181 gcggaagcca gggcttctgc aaaggcttgc tgatcggcga gatcggtgac tgtcacgacg
    15241 caatcggtgt tttcgttaac ccgctgcgcg aaggcgagag ctttatcgaa gaactcatcc
    15301 cgacggttaa agagtttaat ttcttttaaa ccttcaattg ccccctgcgc gccaattgcc
    15361 gttgaggcac caccggcccc taatagcacc atcgttttgc ctttgatatc aaaaccgctc
    15421 tctttaatgg cgcgaatatg acccgtgccg tcggtgttat agccacgcag atagccatca
    15481 tcattaacga tggtgttgat ggctcccacc agtttggccg ctggcgttaa ttcatcaaca
    15541 tattcacacg ccagttgttt gttcggcatt gatacaccag ttccgcgcat tttgagggct
    15601 tttaatcctt caattgctcc gggaaaacta tcgttatcca cttcaaaggc catataggta
    15661 aatggcaatc ccgctttttc taaggcttta ttctgcattt cgggcgataa actgtggcgg
    15721 atgggatagg ccatcaaccc aatcaattcg tattttgcgg taacatccat aattaactcc
    15781 tttaacctct atgcttaatc gtgtttaacc gggtgctaaa tttatgctcg ccaaaccgca
    15841 catcattttc aggaataatg aaaacgcggt aatagcggat aaaaacaata attgcggtaa
    15901 tcagggccag cagcgcgaaa gtaaaatcga gaacaatgat atattgcagg ccgatgttcg
    15961 acagataacc ggtaatcagc ggaataacaa agttagccag tccacccatc atcatatata
    16021 tactggtgac tttggctttg ctttttggga aaaactctga catgaccgaa acgccgagct
    16081 gtaaaatgcc gccagctgct gagaaaccga taacaaaggc tccggcatta cacaccaatg
    16141 gagaagggta caggtagata atggcagctg ttattgttgc cagtgcagag ttaaatacat
    16201 tagcccagat gggccggacc atttttttca gcagggcggc aaaaataaag acacagacca
    16261 gcgaacccat actgtaataa gagatggttt ttaatgcctc agcttctgac ataccagcaa
    16321 aagccatcgc atatttgggc atccacacca caatcacata aaaggtcgcg aatgcggcta
    16381 caccgaacag tactgatgaa acaccttcca gccagactaa cggtttgctg ttcatttgcg
    16441 gtaattcatt agctacgctg gcgtccacca actggctggg gaatttgctt ttcaacagca
    16501 tcagcgtgat cagtacaaac agaatacccg gaataatcag cccatagccg taccagatgt
    16561 tattgagcaa catatagctc accagcattg ggtagaacat ttgcccgaat gacaccatcg
    16621 ctttaaccag tatgaccgcc gaaccagagg ctttcggaaa gcattccata agcgcggggt
    16681 agccacccgt atccagcgct gagttagcga tacctacgca cactgccaga cagtaggcga
    16741 gagttaaatt cgggcaagca ggaataccaa agaagaatag cagatacatt attactgcca
    16801 ttaatatcac cgcccgacga ccaaacttat cggagatcac accgaagaat aaaatactga
    16861 tcaatcgccc caatccgata ccggaaatta aataggcaat gcccgcgttg tcagtggaaa
    16921 acttttctgc caaagatgtc atgttttggg caagcgtaat aacactaatg ccgtgcagga
    16981 aatagctgaa gtaaatacaa agaacagcca ggataaatgg cgtgctgaaa gccttatttt
    17041 gagacatttt cacagctcct tgctgaaaga taatttcgtt accgtacgta ttgttctccg
    17101 acggtttaca taattactat actgaagata attttccctg ggtacatggc ataaagttta
    17161 tgtttccagg aatattgcat gtcatagttt gtttttgatc gcgattttcc ttagttagcg
    17221 cagaaagagt tatttatccg taagatcttg catgacctga aaagaatatt gcattaccca
    17281 cccggagcga cattttcctt tagcgagtga tgccgcgttt ctttgcgact gcgtaagcca
    17341 ataaacagtg ccagtataaa gccgatggca gcgatggcgg tatcgaacca cataatatcg
    17401 gcaatacttc tttgcgaaag atgagccgta atcaacggaa tagtaaaggt tgcaatgctg
    17461 cccgcactgt aatagatccc cgttgctttt cctttagcgt agggaaaacg ttccgccatt
    17521 aacgtcaggc caatttgtac aacaccgcct gcggaggtaa aaccgatgac gaaagcaaat
    17581 attatcacca cataaaatgt gggatgcagg cagacggtga gcagggcgat aaatgagata
    17641 aaggtgtaca gcatcagtaa tgttgttggg cgaacggtat tacgaatgag tggagcggta
    17701 ataaatacac aaagcagcga acccacggtg tagatactga gtaatttgat cgacatggtg
    17761 tatgacatgc cggcgacaaa ttgtccgtat tgtgccagcc actggctaac cagataaaac
    17821 gttgccattg agatatagcc atatagggaa taactggcta aatcgataat tgaacagcga
    17881 tgttccgtag agctggtggt tttctttatg acaggtaagt gacgacccgg atggggtggg
    17941 aacgtacaac ggtataaaag cagagcgtta ataaacataa tgcctgcggc aatcataaag
    18001 gaccaaccga accacagttc agcccacact aacaggctga taattagcgg taataaaaat
    18061 tgtccgctgg aaacaaatgc tttaattaaa atattggctg tacccggtga gcgtggaaaa
    18121 gcttccatca aactgggata ggtgcctgca tcgagaaaac tgtttgccat tcccgccaga
    18181 aagccaaaaa cataagcgat aatgatgtta ttggtgtgca ggatgccaaa aaagaaggcc
    18241 atatagcagc acatcccgag catgataaaa gggcggcgac caaagcgatc ggataataat
    18301 cctgcaaaaa gcaagacact taatcgacca atgcccagtg atgagataac tatcgagacg
    18361 cccgcggcat tagtctgcca aagcgtctcc agcgaggcca tattcaggct catcaaaatg
    18421 acgcccatac catgcaccag gtaattaaaa tacaacccaa gtgcggtagg gtaataggga
    18481 tttttcatag gttctactta gcaaaggtgc tttaaaaagt gtagagatgt tgtgtcagta
    18541 tccgttcatt attgttgtaa caaagacaat tcctttgtgt ggagaaatta acaaagccgc
    18601 tccagatcgt gtgcatataa ttcagcggct accgattgat agatcttcca gtcaataaaa
    18661 ttgccatcag ggtagacttg ttgaaacacg gttaagtcgg ggaaaaaacg catggtgttg
    18721 ttgccgatgc ggttatttat tttctctatg atggcatgaa gatgtttttt tcgttgctga
    18781 cgcatagcca acagttgctc gactacacga tcaggaatgg cgcatttgcc attttcccac
    18841 tgacgccagc tttcactgtc atcggtctgg gcaatccagg tcgcgcattc atcaatagtc
    18901 atggcaaaaa tatggcgaag tgcctggagt tcataagcgt tcatcgtgta atggctgcat
    18961 gaaataaaca ataaaatata aataacatta tcagcctgct gacggcttga agagagtcac
    19021 agtatcttgt gcaacattat tggaaagcgt gaggggcgag atagcaaagc ggaaaaatgt
    19081 taaaaaaaag cccatcgtgg gagatgggca aagactacac acagcaattc gttgtttcac
    19141 tcaggggatt tccatgctta taaatcaata tgttgattta taaccgtgag ctaatagtag
    19201 gcatacggac tttttgcgtc gatcagattc gtctcattag tttaaaaact gtaaagaatt
    19261 tgcgacaggt actaccagct gaatggcggt aagcaggaaa atatggggaa gagcaagcaa
    19321 atatcaatta gcaggggtta acaatcaggc ggcagagccg cctgatgaga aattacttat
    19381 tcgatttctc gatttcgcca agttcttcga gaatttcttc cggttggtcc gtcggcggtg
    19441 ggacttcttc cacccacgcg gcaaaaagac gccaggaaac ggctaacaga accggaccaa
    19501 taaacaggcc gatcatcccg aaagcaatca aaccaccaat aacaccagag aggatcagga
    19561 tcagcggtaa atcggcaccc atgcgaatta acattgggcg gatgacgtta tccagcgtgc
    19621 caaccacacc gctccacact aacaataccg ttccccaggt ggtatcaccg gtccagtaga
    19681 gccagataat cgccggaatc agtaccggca acggtccaag ctggacaagg caggagagga
    19741 tcattaacac cgtgagcaaa gttgcataag gtacgccgga tacggcgagg ccgataccgc
    19801 caagcactgc ctgtactaac gccgtcacca ccacacccag cgccaccgcg cggatagcct
    19861 gtgccgccag tagcacagca gcatcaccgc gaacgcctgc cagacgggtt gcaaaatggc
    19921 gaatgccttg tgccacctgt tcaccgcgcc agtacagcag ggcactgaag agaagcatca
    19981 gcgcacaatg caccataaag cgcccgatat gcgccgcctg cccaacgaac caggtggtgg
    20041 tggtgccaat ataaggacgg actttcgcca tgatcgccgt accccccata tccagcaagt
    20101 tgtgccagcc tgcatatagc ttcgcgccaa tcaccggaat ggtattaagc cacgccagat
    20161 cgggtaacgt catgtcaccg ctggaaatgg ctttaattag cggaccgctg ccatcgacaa
    20221 tactgttaac caacaaggca atagggatga taaacaccat cactaataac agcgtcatca
    20281 ccagaacggc gagagagcgg cggccaaaca tgatcttttg caaacgtaac aataccggcc
    20341 aggtggcgat aaccactgta ccggcccatg caaagccgag aataaagggt tgaacaatcc
    20401 acagacatgc cacaatcatg atggctaaaa acagcaccga aagcagaatt tgtgcgacat
    20461 ccctgggctg acgaacattt accataacta cttttcacct tttttgtgcg tcaaatcgtc
    20521 gacgcgacgt gaacacgcga aactcacctg cataatgatg agcaatttca gcggttttta
    20581 acaggtcgat tctgccctta attctgatgg gcgtgtaaga aaaaaatgtg gtacaacaac
    20641 tcaggcaaca cgcaaacgat ttactcgtcg tatttcaaat tgcatgtgct gcggctacgt
    20701 tcgttcaccc cagtcactta cttttgtaag ctcctggagt tcactcgctt gccgccttcc
    20761 tgcaactcga aatccataga gtaaacaaac atcacctttc agtgcgctat tagcagcctg
    20821 aaagaacagt gtatcgatct acatcacaga ccgcaggaaa gggtcaatat aatgattcca
    20881 cagatttccc aggcacccgg cgtcgttcaa ttggtgctta attttttgca agagctggag
    20941 caacagggtt ttactggcga tacggcgaca agttatgccg atcgtctgac aatgtcgacc
    21001 gacaacagta tttaccaact tctccccgat gcggtggtat ttccgcgttc aaccgcagat
    21061 gtggcgctga tcgcccgtct tgccgcgcag gaacgctatt catcgctgat ctttaccccc
    21121 cgcggcggcg gcaccggcac taacggtcag gcgctcaacc aggggattat tgttgatatg
    21181 tcccgccata tgaaccgcat catcgaaatt aaccctgaag agggctgggt gcgcgttgag
    21241 gccggggtga taaaagatca actcaatcag tacctgaaac cgttcggcta cttttttgcg
    21301 ccggaacttt cgaccagcaa ccgggcaacg ctcggtggga tgatcaatac cgatgcatcc
    21361 ggtcagggat cgctggtcta tggcaaaacg tcagatcacg tacttggcgt acgcgcggtg
    21421 ttgttggggg gcgatattct cgatacgcaa cctttacccg tcgaactggc ggaaacgctg
    21481 ggtaaatcca ataccacaat cgggcgaatt tataacacgg tttatcaacg ttgccgtcag
    21541 caacgccagt taatcatcga caacttcccc aagcttaacc gctttcttac cggttacgat
    21601 ctgcgtcatg tctttaacga tgagatgacc gagttcgacc tgacgcgcat tctgacgggt
    21661 tcagaaggga cgctggcctt tattaccgaa gcgcgactgg atattacgcg cttgcctaaa
    21721 gtgcgccgtc tggtgaacgt caaatatgac tcttttgact ccgcactgcg taacgcgccg
    21781 tttatggttg aggcgcgggc gctttcggta gagacggtgg actcaaaagt gctgaatctg
    21841 gcgcgggaag atattgtctg gcattccgtc agcgagttga ttaccgatgt gcctgacaaa
    21901 gaaatgctcg ggctgaacat tgtggaattt gctggtgatg atgagacgct gattgatgag
    21961 cgggtaaatg cactctgtgc gcggttggat gaactgattg ccagccaaca ggcaggtgtg
    22021 attggctggc aggtgtgccg cgagctggcg ggtgttgaac gtatctatgc gatgcgcaaa
    22081 aaagccgttg gtctgcttgg caatgccaaa ggtgccgcta agccaattcc gtttgctgag
    22141 gatacctgcg taccgccgga acatctggcc gattatattg ctgaatttcg cgcgctgctc
    22201 gacagccacg gcttaagcta cggtatgttc ggtcacgtcg acgcaggtgt cttgcacgtc
    22261 cgtcctgcgc tggatatgtg cgatccgcaa caagagattt tgatgaagca aatctctgat
    22321 gacgtagtgg cgcttaccgc gaaatacggt ggtttgttgt ggggcgaaca cggcaaaggt
    22381 tttcgtgccg aatacagccc tgcgtttttc ggtgaggaac tttttgcaga actgcgcaaa
    22441 gtgaaagcgg catttgaccc gcataaccga ctcaacccag ggaagatttg cccgccagaa
    22501 ggtctcgatg cgccgatgat gaaagtggac gcggtgaagc gcggtacatt cgatcggcag
    22561 atccccattg cggtacgcca gcagtggcgc ggtgcgatgg agtgtaacgg caacggttta
    22621 tgcttcaact ttgatgcccg tagttcgatg tgtccgtcga tgaagatcac ccagaaccgg
    22681 attcattcac cgaaagggcg cgcaacgctg gtgcgtgaat ggctgcgctt gttggcggat
    22741 cgcggcgttg acccactcaa actggaacaa gaactgcctg aatccggcgt cagtttgcgg
    22801 acgttaattg cccgcacgcg caatagctgg catgcgaata aaggcgaata cgacttctca
    22861 cacgaagtca aagaggcgat gtcgggctgt cttgcctgta aagcgtgttc gacccagtgc
    22921 cccatcaaaa ttgatgtgcc ggagtttcgc tctcgttttc tgcagctcta tcacacccgt
    22981 tatttacgcc cgcttcgtga ccacctcgtt gctacggtcg agagctacgc accgctgatg
    23041 gcacgcgcgc cgaaaacctt taacttcttc attaaccagc cgctggtgcg caagctctcg
    23101 gaaaaacata tcggcatggt tgatttgcca ctgctgtcag tcccctcgct acaacaacaa
    23161 atggtggggc atcgctcggc gaatatgacg ctggaacagc ttgaagctct caatgcagag
    23221 cagaaagcgc gcacggtgtt ggtggtgcag gaccccttta ccagctatta cgatgcgcaa
    23281 gtggtggcgg attttgtccg tctggttgaa aaattaggtt tccagcctgt gttactgcca
    23341 ttttcgccaa atggcaaagc ccagcatatc aaaggctttc ttaatcgttt tgcgaagacg
    23401 gcgaaaaaga cggcggattt cctcaaccgt atggcgaagc tggggatgcc aatggtgggc
    23461 gtcgatccgg cgctggtact ttgttatcgc gatgaatata aactggcgct gggcgaggaa
    23521 cgtggcgagt ttaacgtctt actggcgaat gaatggctgg caagcgcact tgactcacag
    23581 ccagtggcta cagtcagcgg tgaatcatgg tatttctttg gtcactgtac cgaagttacc
    23641 gctttgccgg gcgcgccagc acaatgggtt gcgatatttg cccgttttgg cgcgaaactg
    23701 gaaaatgtca gcgtggggtg ctgcggcatg gcagggactt acggacatga agcgaaaaac
    23761 cataaaaatt cgctcgggat ctatgagtta tcctggcatc aggcgatgca gcgactgccg
    23821 cgtaatcgct gtctggcgac cggatattcc tgccgtagcc aggtaaaacg ggtcgaaggc
    23881 acgggggtac gccatcctgt gcaggcttta ctggagatta ttaaatgata tggaaacgaa
    23941 aaatcaccct ggaagcactg aatgccatgg gtgaaggaaa catggtgggg ttgctggata
    24001 ttcgctttga acatattggt gatgacaccc ttgaagcgac aatgccagta gactcgcgga
    24061 caaagcagcc tttcgggttg ctgcatggag gggcatccgt ggtactggcc gaaagtatcg
    24121 gttccgttgc cggttattta tgtaccgaag gtgagcaaaa ggtggttggt ctggaaatca
    24181 atgctaacca cgtccgctcg gcacgagaag ggcgggtgcg cggcgtatgc aaaccgttgc
    24241 atctcggttc gcgtcaccag gtctggcaga ttgaaatctt cgatgagaaa gggcgtttgt
    24301 gctgttcgtc acgattaacg accgccattt tgtgacgttg tcaccagaaa agtgtgacgc
    24361 atattgcttt tggtcattat ctgcacagta aacggtggta tactgtgcag gtcttgtcta
    24421 aaagcgagga tgctatgtct actcaacttg atcccaccca gctggccatt gaatttttac
    24481 gccgtgacca gagcaatctt tcacctgccc aatatctcaa acgtctgaaa caactagaac
    24541 ttgaatttgc tgacctgctt actctctctt ctgctgaatt aaaagaagaa atctactttg
    24601 cctggcgtct gggcgtgcat tgatcctgac ttaatgccgc aattttgcca aatcgacctg
    24661 gattaacctc cgtccaggtt cgatgacctt cttaatactg cttatctatc cgcctgatat
    24721 tgttcaaaaa taatactaat cgcagtttgt gttaaaacgg cgggttagct ttatgagatt
    24781 attcttatcg ccccttcaag agctaagcca ctgtgagtgc cggagataag cgccggatgg
    24841 ggtagaaacc cttaagcctg tgtcgcacag acttaagggt tttcttattt ctgaacgctg
    24901 cactttttta cctatccctt aaatagtcat tttcaataca actttttctt tttcatcaca
    24961 gccttaatgc tccttagata ataatcatta tctaacaatg agatacctaa ttcttagcgt
    25021 gcctgttaac ccacacatca gggtctatgc ttattaaaag caggtaaaaa gcatgtttta
    25081 cgctaaagtc cctgcgtttg ctgggttgaa ctgataatca ttatcactaa catgctgtta
    25141 tacgctgaaa gcgatgaagt gaggtaaatc gatggacatg cattcaggaa cctttaatcc
    25201 acaggatttc gcctggcagg gcttaacgct gacacccgca gccgctgtcc acatccgcga
    25261 gctggtggca aagcagccgg gtatggtcgg cgtgcgctta ggcgtgaagc aaacgggctg
    25321 cgcgggcttt ggctatgtgc tcgacagtgt tagcgagccg gacaaagacg atctgctgtt
    25381 tgaacacgac ggcgcgaagc tgtttgtccc gctgcaagcg atgccgttta ttgatggcac
    25441 ggaagtcgat ttcgttcgtg aaggacttaa tcagatattc aaatttcaca accctaaagc
    25501 ccagaatgaa tgtggctgtg gcgaaagctt tggggtatag gcggtactat gtctcgtaat
    25561 actgaagcaa ctgacgatgt caaaacctgg accggcggcc cgctgaatta taaagaagga
    25621 ttcttcaccc agttagccac cgatgagctg gcaaagggga taaacgaaga ggtggtgcgc
    25681 gcaatttcgg cgaagcgtaa tgagccggag tggatgctgg agtttcgtct aaatgcctat
    25741 cgcgcatggc tggagatgga agaaccgcac tggttgaaag cgcactacga caagctgaat
    25801 tatcaggatt acagctacta ttcggcacca tcgtgcggta attgtgacga cacttgtgcg
    25861 tctgaacccg gcgcggtgca gcaaactggc gcgaacgcct ttttaagtaa agaggtggag
    25921 gcggcgtttg agcagttggg cgttcccgtg cgggaaggca aagaggtggc agtggatgcc
    25981 attttcgact ctgtttcggt tgccactact tatcgcgaaa aactggcgga gcagggaatt
    26041 attttttgtt cctttggcga ggcgatccac gatcacccgg aactggtgcg taaatatctc
    26101 ggcaccgtgg tgccggggaa tgacaacttc tttgccgcac ttaatgcggc ggtagcctct
    26161 gacggtacgt ttatttatgt gcctaaaggc gtgcgttgcc cgatggaact ttccacctat
    26221 tttcgcatta acgcggaaaa aaccgggcag tttgagcgca ccattctggt ggccgacgaa
    26281 gacagctacg ttagctacat tgaaggctgt tccgctccgg tgcgtgacag ctatcagtta
    26341 cacgcggcgg tggtggaagt catcatccat aaaaacgccg aggtgaaata ttccacggta
    26401 caaaactggt tccctggcga taacaacacc ggcggtattc tcaacttcgt caccaagcgt
    26461 gcgttgtgcg aaggcgaaaa cagcaaaatg tcatggacgc aatcagaaac cgggtcagcg
    26521 attacgtgga aatatcccag ctgcattttg cgcggcgata actccattgg tgagttttac
    26581 tcagtggcgc tgaccagcgg tcatcagcaa gcggataccg gcaccaagat gatccacatc
    26641 ggtaaaaaca ccaaatcgac cattatctcg aaagggatct ctgccggaca tagtcagaac
    26701 agttatcgcg gcttagtgaa aatcatgccg acggcaacca atgcgcgcaa tttcactcag
    26761 tgcgattcaa tgctgattgg cgctaattgt ggggcgcata ccttcccgta tgtcgaatgt
    26821 cgcaataaca gcgctcagct ggagcacgag gcaacgacat cacgtattgg tgaagatcaa
    26881 ctgttttact gcctgcaacg cgggatcagc gaagaagatg ccatctcgat gattgttaac
    26941 ggtttttgca aagacgtgtt ctcggaactg ccgctggaat ttgccgttga agcacaaaaa
    27001 ctcctcgcca tcagtcttga acacagcgtc ggataaggaa taaacatgtt aagtattaaa
    27061 gatttacacg tcagcgtgga agataaagct atcctgcgtg gattaagcct cgacgttcgt
    27121 cccggcgaag ttcacgccat tatggggcca aacggttctg gcaaaagtac cttatcggca
    27181 acgcttgccg ggcgagaaga ttatgaggtg acgggcggca cggttgagtt caaaggcaaa
    27241 gatttgcttg cgctgtcacc ggaagatcgt gcaggtgaag gcatctttat ggccttccag
    27301 tatccggtgg aaattcccgg cgtcagtaac cagtttttcc tgcaaacggc acttaatgcg
    27361 gtgcgcagct atcgcggcca ggaaacgctc gaccgctttg attttcagga tttgatggaa
    27421 gagaaaatcg ctctcctgaa gatgccggaa gatttattaa cccgttcggt aaacgttggt
    27481 ttttccggcg gtgagaaaaa gcgcaacgat attttgcaaa tggcggtgct ggaaccggag
    27541 ttatgcattc ttgatgagtc agactccggg ctggatattg acgcattaaa agtggttgcc
    27601 gatggcgtga actcgctgcg tgacggcaag cgttcattca tcattgttac gcactaccaa
    27661 cgcattctcg actacatcaa gcctgattac gtccatgtgc tgtatcaggg acgaattgtg
    27721 aaatccggcg atttcacgtt ggtcaaacaa ctggaggagc agggttatgg ctggcttacc
    27781 gaacagcagt aacgcactgc aacagtggca tcatttgttt gaagctgaag gggcgaaacg
    27841 ctccccgcaa gcacagcagc atttacaaca attgctgcgt accggactgc cgacacgtaa
    27901 acatgaaaac tggaaatata cgccgctgga cgggctgacc aatagccagt ttgtcagcat
    27961 tgcgggggag atatccccac agcagcgtga tgccttagcg ttaacattag acgccgtgcg
    28021 gctggtgttt gtcgatggac gttacgtgcc cgcactgagc gatgcaactg aaggcagcgg
    28081 atatgaagtg agcattaacg acgaccgtca ggggttaccc gatgctattc aggcggaagt
    28141 gtttctgcat ttgacggaaa gcctggcaca aagcgtgacg catatcgccg tgaagcgcgg
    28201 tcaacggccg gcaaagccat tgctgttaat gcatatcacc cagggcgtgg caggtgaaga
    28261 ggtgaatact gcccattacc ggcatcatct ggatctggcg gaaggtgccg aagcaacggt
    28321 gatcgaacat tttgtcagcc ttaatgatgc tcgtcacttc accggcgcac ggttcactat
    28381 caacgtcgca gcgaacgccc atttgcagca tatcaagctg gcgtttgaaa acccgctcag
    28441 tcaccacttt gctcataacg atttgttgct ggctgacgat gccaccgcat ttagccacag
    28501 tttcctgctg ggtggcgcag tattacgaca caacaccagt acgcaactca atggcgaaaa
    28561 cagcacgctg cggatcaata gcctggcgat gccggtgaaa aacgaggtgt gtgatactcg
    28621 tacctggttg gaacacaata aaggtttttg taacagccga cagttgcaca aaactatcgt
    28681 cagcgacaaa ggccgcgcgg tatttaacgg tttgatcaac gtcgcgcagc acgccatcaa
    28741 aacggatggt cagatgacca acaataatct gctgatgggc aaactggcgg aagtggatac
    28801 gaaaccgcag ctggaaatct atgcagatga tgtgaaatgc agccacggcg cgacggtggg
    28861 gcgtattgat gacgaacaga tgttttatct gcgctcgcgc gggatcaatc agcaagatgc
    28921 ccagcagatg atcatttacg cctttgctgc cgaactgacg gaagcactgc gtgatgaggg
    28981 gcttaaacag caggtgctgg cccgaatcgg tcaacgactg ccaggaggtg caagatgact
    29041 ttttccgtcg acaaagtgcg ggccgacttt ccggtgcttt ctcgtgaggt aaacggtttg
    29101 ccgctggctt atctcgacag cgccgccagt gcgcagaaac cgagccaggt gattgatgcc
    29161 gaggctgagt tttatcgtca tggctacgcg gcggtgcatc gcggtattca taccttaagc
    29221 gcccaggcga ccgagaaaat ggagaacgtg cgcaagcggg catcgctgtt tattaatgcc
    29281 cgttcggcgg aagagctggt gttcgtccgc ggcacgacgg aagggatcaa tctggtcgcc
    29341 aatagctggg gcaacagcaa tgtgcgagcg ggcgataaca tcatcatcag tcagatggag
    29401 caccacgcta acattgtccc ctggcagatg ctttgtgcac gcgttggcgc agagctgcgt
    29461 gtgatcccgc tcaaccccga cggtactctg caactggaga cgctgcctac gctgtttgat
    29521 gagaaaactc gtctgctggc aattactcat gtctccaacg tgcttgggac agaaaatccg
    29581 ctggcggaga tgatcacact tgcgcatcaa catggcgcaa aagtgctggt ggatggcgct
    29641 caggcggtga tgcatcatcc ggtggatgtt caggcgctgg attgcgattt ttacgtgttc
    29701 tccgggcata aactgtatgg ccctaccgga attggcattc tttatgtcaa agaagccttg
    29761 ttgcaggaga tgccgccgtg ggaagggggc ggttctatga tcgccaccgt cagcctgagt
    29821 gaaggcacta cctggaccaa agcaccatgg cggtttgaag ccggtacacc caataccgga
    29881 ggcatcattg gtcttggcgc ggcgctggag tatgtttcgg cgctggggct taataacata
    29941 gccgagtatg aacagaatct gatgcattac gcgttatcac agttggaatc tgtaccggat
    30001 ctcactctct atggcccaca aaacaggctt ggcgttattg cttttaatct cggtaaacac
    30061 cacgcctatg atgttggcag ttttctcgat aattacggca ttgctgtgcg taccggacat
    30121 cactgcgcaa tgccattaat ggcctattac aacgtccctg cgatgtgtcg ggcatcgctg
    30181 gccatgtata acacccatga agaagtggat cgtctggtga ccggcctgca acgtattcac
    30241 cgtctgctgg gataacaggg aggcactatg gctttattgc cggataaaga aaaattgctg
    30301 cgtaattttt tacgctgcgc caactgggaa gagaaatatc tctacattat tgagttgggt
    30361 cagcgtctgc cagaattacg tgacgaagac aaaagcccgc aaaatagcat ccagggctgc
    30421 cagagtcagg tgtggattgt gatgcgtcaa aatgcgcagg gaattattga attacagggc
    30481 gacagcgatg cggccattgt aaaagggctt attgcggtcg tctttattct ttacgatcaa
    30541 atgacgccgc aggatattgt caatttcgat gtgcgcccgt ggtttgaaaa aatggcgctc
    30601 acccaacatc tcaccccatc acgctcacaa ggtctggaag cgatgattcg cgcaattcgc
    30661 gccaaagccg ctgcacttag ctaaactaaa gggacagctt tcatccggtt ggctcacatt
    30721 gccgggtggt caatgtttac actggcctgc acaggagtgt tttggtttca ggtgaacata
    30781 agcgccatcc gacgtttcag cgtgagtctc cggcaaatac aggaggttta caatgaaatg
    30841 cgcgtctttg cttacactca cgcttatcgg cgcttttagc gccatccagg ctgcctgggc
    30901 ggttgattat ccgctaccac caaccggcag tcgactggtt gggcaaaatc aaacgtatac
    30961 ggtgcaagaa ggggataaaa accttcaggc catcgcccga cgttttgata ctgcggcaat
    31021 gttgatcctt gaagccaata acactatcgc cccggtgcca aaacccggta cgacgataac
    31081 tattccttca caactattat tacctgatgc gccgcgtcag gggattattg taaaccttgc
    31141 agagctgcgc ctttataatt atccgccggg agaaaatatc gtgcaggttt atccgatagg
    31201 tattggtttg caggggctgg aaacgccggt gatggaaacg cgtgtggggc agaaaatccc
    31261 taacccaacc tggacgccta cggcgggcat tcgtcaacgt tcgctggaac gtggcattaa
    31321 attaccacca gtcgttcctg ccgggcccaa taacccacta ggacgttacg cactgcgcct
    31381 cgcgcatggt aatggcgaat acctcattca cggcaccagt gcgccggaca gcgtcggttt
    31441 gcgcgtcagt tcagggtgta ttcgcatgaa tgcaccggat attaaagcct tattctccag
    31501 cgtgcggacg ggaacgccgg tgaaagtgat caacgaaccg gtgaaatatt ccgtggagcc
    31561 taacgggatg cgttatgttg aagtacatcg accactatcg gcagaagaac agcagaacgt
    31621 tcagacaatg ccatacacac tgccagcagg ctttacgcaa tttaaagaca ataagggtgt
    31681 agatcagaaa ttagtcgata aagcgttgta tcgtcgggca gggtatccgg ttgcggtgag
    31741 cagtggagca actcccgcag ccagcaatgc gccttcagta gagtcagcgc agaatggtga
    31801 accagagcaa gggaatatgt tacgcgcgac gcagtagcgg taaatggcag gcaaaaaaaa
    31861 tggcgcacaa tgtgcgccat ttttcacttc acaggtacta ttacttgcgg tatttagtag
    31921 ccatgttgtc cagacgctgg ttagcacgag ctgcgtcatc tttagcagcc tgaacgtcgg
    31981 aacgcattgc gttcacgtcg ttgctcagct ggtcaacttt agcgttcaga gtctgaacgt
    32041 cagaagacag ctgatcgatt ttagcgttgc tggagcaacc tgccagcaga gtagaaccca
    32101 ggattaccgc gcccagtacc agtttagtag ctttcattat taataccctc ta
//
LOCUS       DAZFLD010000025        31740 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.025, whole genome shotgun sequence.
ACCESSION   DAZFLD010000025 DAZFLD010000000
VERSION     DAZFLD010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31740
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.025"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            117..2756
                     /gene="ydbH"
                     /locus_tag="ACHKBL_001041"
     CDS             117..2756
                     /gene="ydbH"
                     /locus_tag="ACHKBL_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH family protein"
                     /protein_id="HFO5817952.1"
                     /translation="MLGKYKAVLALLLLIILVPLTLLMTLGLWVPTLAGIWLPLGTRI
                     ALDESPRITRKGLIIPDLRYLVGDCQLAHITNASLSHPSRWLLNVGTVELDSACLAKL
                     PQTEQSPAAPKTLAQWQAMLPNTWINIDKLIFSPWQEWQGKLSLALTSDIQQLRYQGE
                     KVKFQGQLKGQQLTVSELDVVAFENQPPVKLVGEFTMPLVPDGLPVSGHATATLNLPQ
                     EPSLVDAELDWQENSGQLIVVARDNGDPLLDLPWQITRQQLTVSDGRWSWPYAGFPLS
                     GRLGVKVDNWQAGLENALVSGRLSVLTQGQAGKGNAVLNFGPGKLSMDNSQLPLQLTG
                     EAKQADLILYARLPAQLSGSLTDPTLAFEPGALLRSKGRVIDSLDIDEIRWPLAGVKV
                     TQRGVDGHLQAILQAHENELGDFVLHMDGLANDFLPDAGRWQWRYWGKGSFTPMNATW
                     DVAGKGEWHDSTITLTDLSTGFDQLQYGTMTVEKPRLILDKPVVWVRDAQHPSFSGAL
                     SLDAGQTLFTGGSVLPPSTLKFSVDGRDPTYFLFKGDLHAGEIGPVRVNGRWDGIRLR
                     GNAWWPKQSLTVFQPLVPPDWKMNLRDGELYAQVAFSAAPEQGFRAGGHGVLKGGSAW
                     MPDNQVNGVDFVLPFRFADGAWHLGTRGPVTLRIAEVINLVTAKNITADLQGRYPWSE
                     DEPLLLTDVSVDVLGGNVLMKQLRMPQHDSALLRLNNLSSSELVSAVNPKQFAMSGAF
                     SGALPLWLNNEKWIVKDGWLANSGPMTLRLDKDTADAVVKDNMTAGSAINWLRYMEIS
                     RSSTKINLDNLGLLTMQANITGTSRVDGKSGTVNLNYHHEENIFTLWRSLRFGDNLQA
                     WLEQNAHLPGNDCPQGKECEDKQ"
     gene            2753..2938
                     /gene="ynbE"
                     /locus_tag="ACHKBL_001042"
     CDS             2753..2938
                     /gene="ynbE"
                     /locus_tag="ACHKBL_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HFO5817953.1"
                     /translation="MKILLAALTSSFMLVGCTPRIEVAAPKEPITINMNVKIEHEIII
                     KADKDVEELLETRSDLF"
     gene            2946..3272
                     /gene="ydbL"
                     /locus_tag="ACHKBL_001043"
     CDS             2946..3272
                     /gene="ydbL"
                     /locus_tag="ACHKBL_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HFO5817954.1"
                     /translation="MKRTLLLCAFLVGLVSSNVMALTLDEARTQGRVGETFYGYLVAL
                     KTDAETEKLVTDINAERKASYQQLAKQNNVSVDDIAKLAGQKLVERAKPGEYVQGING
                     KWVRKF"
     gene            complement(3444..3650)
                     /locus_tag="ACHKBL_001044"
                     /pseudo
     CDS             complement(3444..3650)
                     /locus_tag="ACHKBL_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415902.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            3651..3803
                     /locus_tag="ACHKBL_001045"
                     /pseudo
     CDS             3651..3803
                     /locus_tag="ACHKBL_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415917.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation operon negative
                     regulatory protein PaaX"
     gene            3785..4375
                     /gene="paaY"
                     /locus_tag="ACHKBL_001046"
     CDS             3785..4375
                     /gene="paaY"
                     /locus_tag="ACHKBL_001046"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation protein PaaY"
                     /protein_id="HFO5817955.1"
                     /translation="MPIYQIDGLTQFVPEESFVHPTAVLIGDVILGKGVYVGPNTSLR
                     GDFGRIVVKDGANIQDNCVMHGFPDQDTVVEEDGHIGHSAILHGCIIHRNALVGMNAV
                     VMDGAVIGENSIVGASAFVKAKAEMPANYLIVGSPAKAIRELSEQELAWKKQGTHEYQ
                     VLVTRSKQTLHQVEPLREVEPGRQRLVFDENLRPKQ"
     gene            4606..5466
                     /gene="pdxI"
                     /locus_tag="ACHKBL_001047"
     CDS             4606..5466
                     /gene="pdxI"
                     /locus_tag="ACHKBL_001047"
                     /EC_number="1.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 4-dehydrogenase"
                     /protein_id="HFO5817956.1"
                     /translation="MSSNTFTLGTKSVNRLGYGAMQLAGPGVFGPPRDRHVAITVLRE
                     ALALGVNHIDTSDFYGPHVTNQIIREALYPYSDDLTIVTKIGARRGEDASWLPAFSPA
                     ELQKAVHDNLRNLGLGALDVVNLRVMMGDGHGPAEGSIEASLTVLAEMQQQGLVKHIG
                     LSNVTPTQVAEARKITEIVCVQNEYNIAHRADDAMIDALARDGIAYVPFFPLGGFTPL
                     QSSTLSDVAASLGATPMQVALAWLLQRSPNILLIPGTSSVAHLRENMAAEKLHLSEEV
                     LSTLDGISRE"
     gene            5530..6860
                     /locus_tag="ACHKBL_001048"
                     /pseudo
     CDS             5530..6860
                     /locus_tag="ACHKBL_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310036.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing bactofilin"
     gene            7029..7634
                     /gene="ynbA"
                     /locus_tag="ACHKBL_001049"
     CDS             7029..7634
                     /gene="ynbA"
                     /locus_tag="ACHKBL_001049"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFO5817957.1"
                     /translation="MTLYQIKPLFQSLLRPTMFWLHKRQVSANQITLAALALSLLTGL
                     LLALVPYHGMFLLLPIVLFVRMALNALDGMMARECNQQTRLGAILNETGDVISDIALY
                     LPFLFLPESNASLVILMLFCTILTEFCGLLAQTINGIRSYAGPFGKSDRALIFGLWVL
                     AVAIYPQWMQWNNLLWSIASILLLWTAINRCRSVLLMNAEK"
     gene            7634..8529
                     /gene="ynbB"
                     /locus_tag="ACHKBL_001050"
                     /pseudo
     CDS             7634..8529
                     /gene="ynbB"
                     /locus_tag="ACHKBL_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310038.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
     gene            8545..10302
                     /gene="ynbC"
                     /locus_tag="ACHKBL_001051"
     CDS             8545..10302
                     /gene="ynbC"
                     /locus_tag="ACHKBL_001051"
                     /EC_number="2.1.1.-"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha/beta hydrolase/class I
                     SAM-dependent methyltransferase"
                     /protein_id="HFO5817958.1"
                     /translation="MENSRIPGEHFFTTSDNTALFYRHWPTLQPGAKKVIVLFHRGHE
                     HSGRLQHLVDELAMPDTAFYAWDARGHGQTSGPRGYSPSLASSVQDVDEFVRFAASDS
                     QVGLEEVVVIAQSVGAVMVATWVHDYAPAIRGLVLASPAFKVKLYVPLARPALALWHR
                     LRGLFFINSYVKGRYLTHDRQRVASFNNDPLITRAIAVNILLDLYKTSERIISDAAAI
                     TLPTQLLISGDDYVVHRQPQIDFYQRLRSPLKELHLLPGFYHDTLGEENRALVFEKMQ
                     SFISHLYANKSQKLDYQHEDCTGPSADRWRLLSGGPVPLSPVDLAYRFMRKAMKLFGA
                     HSAGLHLGMSTGFDSGSSLDYVYQNQPQGSNAFGRLVDKIYLNSVGWRGIRQRKTHLQ
                     MLIKQAVADLHAKGLAVRVVDIAAGHGRYVLDALANEPAVSDIFLRDYSELNVAQGQE
                     MIAQRGMSGRVRFEQGDAFNPEELSALTPRPTLAIVSGLYELFPENEQVKNSLAGLAN
                     AIDPGGILIYTGQPWHPQLELIAGVLTSHKDGKPWVMRVRSQGEMDSLVHDAGFDKCT
                     QRIDEWGIFTVSMAVRRGN"
     gene            10316..11608
                     /gene="ynbD"
                     /locus_tag="ACHKBL_001052"
                     /pseudo
     CDS             10316..11608
                     /gene="ynbD"
                     /locus_tag="ACHKBL_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415929.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2/dual specificity phosphatase
                     family protein"
     gene            complement(11662..12267)
                     /gene="azoR"
                     /locus_tag="ACHKBL_001053"
     CDS             complement(11662..12267)
                     /gene="azoR"
                     /locus_tag="ACHKBL_001053"
                     /EC_number="1.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000048941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HFO5817959.1"
                     /translation="MSKVLVLKSSILAGYSQSNQLSDYFVEQWREKHSADEITVRDLA
                     ANPIPVLDGELVGALRPSDAPLTPRQQEALALSDELIAELKAHDVIVIAAPMYNFNIS
                     TQLKNYFDLVARAGVTFRYTENGPEGLVTGKKAIVITSRGGIHKDGPTDLVTPYLSTF
                     LGFIGITDVKFVFAEGIAYGPEMAAKAQSDAKAAIDSIVAE"
     gene            12468..16370
                     /gene="hrpA"
                     /locus_tag="ACHKBL_001054"
     CDS             12468..16370
                     /gene="hrpA"
                     /locus_tag="ACHKBL_001054"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HFO5817960.1"
                     /translation="MTEQQKLTFTALQQRLDSLMLRDRLRFSRRLHGVKKVKNPDAQQ
                     AIFQEMAKEIDHAAGKVLLREAARPEITYPDNLPVSQKKQDILEAIRDHQVVIVAGET
                     GSGKTTQLPKICMELGRGIKGLIGHTQPRRLAARTVANRIAEELKTEPGGCIGYKVRF
                     SDHVSDNTMVKLMTDGILLAEIQQDRLLMQYDTIIIDEAHERSLNIDFLLGYLKELLP
                     RRPDLKIIITSATIDPERFSRHFNNAPIIEVSGRTYPVEVRYRPIVEEADDTERDQLQ
                     AIFDAVDELSQESPGDILIFMSGEREIRDTADALNKLNLRHTEILPLYARLSNSEQNR
                     VFQSHSGRRIVLATNVAETSLTVPGIKYVIDPGTARISRYSYRTKVQRLPIEPISQAS
                     ANQRKGRCGRVSEGICIRLYSEDDFLSRPEFTDPEILRTNLASVILQMTALGLGDIAA
                     FPFVEAPDKRNIQDGVRLLEELGAITTDEQASAYKLTPLGRQLSQLPVDPRLARMVLE
                     AQKHGCVREAMIITSALSIQDPRERPMDKQQASDEKHRRFHDKESDFLAFVNLWNYLG
                     EQQKALSSNAFRRLCRTDYLNYLRVREWQDIYTQLRQVVKELGIPVNSEPAEYREIHI
                     ALLTGLLSHIGMKDADKQEYTGARNARFSIFPGSGLFKKPPKWVMVAELVETSRLWGR
                     IAARIDPEWVEPVAQHLIKRTYSEPHWERAQGAVMATEKVTVYGLPIVAARKVNYSQI
                     DPALCRELFIRHALVEGDWQTRHAFFRENLKLRAEVEELEHKSRRRDILVDDETLFEF
                     YDQRISHDVISARHFDSWWKKVSRETPDLLNFEKSMLIKEGAEKISKLDYPNFWHQGN
                     LKLRLSYQFEPGADADGVTVHIPLPLLNQVEESGFEWQIPGLRRELVIALIKSLPKPV
                     RRNFVPAPNYAEAFLGRVTPLELSLLDSLERELRRMTGVTVDREDWHWDQVPDHLKIT
                     FRVVDDKNKKLKEGRSLQDLKDALKGKVQETLSAVADDGIEQSGLHIWSFGQLPESYE
                     QKRGNYKVKAWPALVDERDSVAIKLFDNPLEQKQAMWNGLRRLLLLNIPSPIKYLHEK
                     LPNKAKLGLYFNPYGKVLELIDDCISCGVDQLIDANGGPVWTEEGFAALHEKVRAELN
                     DTVVDIAKQVEQILTAVFNINKRLKGRVDMTMALGLSDIKAQMGGLVYRGFVTGNGFK
                     RLGDTLRYLQAIEKRLEKLAVDPHRDRAQMLKVENVQQAWQQWINKLPPARREDEDVK
                     EIRWMIEELRVSYFAQQLGTPYPISDKRILQAMEQISG"
     gene            16642..17442
                     /gene="ydcF"
                     /locus_tag="ACHKBL_001055"
     CDS             16642..17442
                     /gene="ydcF"
                     /locus_tag="ACHKBL_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF218 domain-containing protein YdcF"
                     /protein_id="HFO5817961.1"
                     /translation="MNITPFPTLSPATIDAINVIGQWLAQDDFSGEMPYQADCVILAG
                     NAVMPTIDAACKIARDQQIPLLISGGIGHSTTFLYSAIAQHPHYNTIRTTGRAEATIL
                     ADIAHQFWHIPHEKIWIEDQSTNCGENARFSIALLNQAVERVHTAIVVQDPTMQRRTM
                     ATFRRMTGDNPDAPRWLSYPGFVPQLGNNADSVIFVNPLQGLWPVERYLSLLTGELPR
                     LRDDSDGYGPRGRDFIVHVDFPAEVIHAWQTLKHDAVLIEAMESRSLR"
     gene            17639..19078
                     /gene="aldA"
                     /locus_tag="ACHKBL_001056"
     CDS             17639..19078
                     /gene="aldA"
                     /locus_tag="ACHKBL_001056"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HFO5817962.1"
                     /translation="MSVPVQHPMYIDGQFVTWRGDAWIDVVNPATEAVISRIPDGQAE
                     DARKAIDAAERAQPEWEALPAIERASWLRKISAGIRERASEISTLIVEEGGKIQQLAE
                     VEVAFTADYIDYMAEWARRYEGEIIQSDRPGENILLFKRALGVTTGILPWNFPFFLIA
                     RKMAPALLTGNTIVIKPSEFTPNNAIAFAKIVDEIGLPRGVFNLVLGRGETVGQELAG
                     NPKVAMVSMTGSVSAGEKIMATAAKNITKVCLELGGKAPAIVMDDADLELAVKAIVDS
                     RVINSGQVCNCAERVYVQKGIYDQFVNRLGEAMQAVQFGNPAERNDIAMGPLINAAAL
                     ERVEQKVARAVEEGARVALGGKAVEGKGYYYPPTLLLDVRQEMSIMHEETFGPVLPVV
                     AFDTLEEAISMANDSDYGLTSSIYTQNLNVAMKAIKGLKFGETYINRENFEAMQGFHA
                     GWRKSGIGGADGKHGLHEYLQTQVVYLQS"
     gene            complement(19119..20120)
                     /gene="gap"
                     /locus_tag="ACHKBL_001057"
     CDS             complement(19119..20120)
                     /gene="gap"
                     /locus_tag="ACHKBL_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFO5817963.1"
                     /translation="MSKVGINGFGRIGRLVLRRLLEVKSNIDVVAINDLTSPKILAYL
                     LKHDSNYGPFPWSVDFTEDSLIVDGKSIAVYAEKEAKNIPWKAKGAEIIVECTGFYTS
                     AEKSQAHLDAGAKKVLISAPAGEMKTIVYNVNDDTLDGNDTIVSVASCTTNCLAPMAK
                     ALHDSFGIEVGTMTTIHAYTGTQSLVDGPRGKDLRASRAAAENIIPHTTGAAKAIGLV
                     IPELSGKLKGHAQRVPVKTGSVTELVSILAKKVTAEEVNNALKQATTNNESFGYTDEE
                     IVSSDIIGSHFGSVFDATQTEITAVGDLQLVKTVAWYDNEYGFVTQLIRTLEKFAKL"
     gene            20309..20839
                     /gene="cybB"
                     /locus_tag="ACHKBL_001058"
     CDS             20309..20839
                     /gene="cybB"
                     /locus_tag="ACHKBL_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b561"
                     /protein_id="HFO5817964.1"
                     /translation="MGNKYSRLQISIHWLVFLLVIVAYCAMEFRGFFPRSDRPLINMI
                     HVSCGISILVLMVVRLLLRLKYPTPPIIPKPKPMMTGLAHLGHLVIYLLFIALPVIGL
                     VMMYNRGNPWFAFGLTMPYASEANFERVDSLKSWHEMLANLGYFVIGLHAAAALAHHY
                     FWKDNTLLRMMPRKRS"
     gene            21084..21257
                     /locus_tag="ACHKBL_001059"
     CDS             21084..21257
                     /locus_tag="ACHKBL_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5817965.1"
                     /translation="MKKLALILVIGTLVSFYADAGRKPCSGSKGGISHCTAGGKFVCN
                     DGSISASKKTCIN"
     gene            21283..21351
                     /gene="yncP"
                     /locus_tag="ACHKBL_001060"
     CDS             21283..21351
                     /gene="yncP"
                     /locus_tag="ACHKBL_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YncP"
                     /protein_id="HFO5817966.1"
                     /translation="MNLLVRCAGKIPALALTWTCRP"
     gene            complement(21329..21478)
                     /gene="hokB"
                     /locus_tag="ACHKBL_001061"
     CDS             complement(21329..21478)
                     /gene="hokB"
                     /locus_tag="ACHKBL_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokB"
                     /protein_id="HFO5817967.1"
                     /translation="MKRNPLVVCLLIICITILTFTLLTRQTLYELRFRDGDKEVAALM
                     ACTSR"
     gene            21877..23517
                     /gene="trg"
                     /locus_tag="ACHKBL_001062"
     CDS             21877..23517
                     /gene="trg"
                     /locus_tag="ACHKBL_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein Trg"
                     /protein_id="HFO5817968.1"
                     /translation="MNTTPSQRLGFLHHIRLVPLFACILGGILVLFALSSALAGYFLW
                     QADRDQRDVTAEIEIRTGLANSSDFLRSARINMIQAGAASRIAEMEAMKRNIAQAESE
                     IKQSQQGYRAYQNRSVKTPADEALDTELNQRFQAYITGMQPMLKYAKNGMFEAIINHE
                     SEQIRPLDNAYTDILSKAVKIRSTRANQLAELAHQRTRLGGMFMIGAFVLALVMTLIT
                     FMVLRRIVIRPLQHAAQRIEKIASGDLTMNDEPAGRNEIGRLSRHLQQMQHSLGMTVG
                     TVRQGAEEIYRGTSEISAGNADLSSRTEEQAAAIEQTAASMEQLTATVKQNADNAHHA
                     SKLAQEASIKASDGGQTVSGVVKTMGAISTSSKKISEITAVINSIAFQTNILALNAAV
                     EAARAGEQGRGFAVVASEVRTLASRSAQAAKEIEGLISESVRLIDLGSDEVATAGKTM
                     STIVDAVASVTHIMQEIAAASDEQSRGITQVSQAISEMDKVTQQNASLVEEASAAAVS
                     LEEQAARLTEAVDVFRLNKHSVSAEPRGAGEPVSFATV"
     gene            complement(23555..24478)
                     /gene="ydcI"
                     /locus_tag="ACHKBL_001063"
     CDS             complement(23555..24478)
                     /gene="ydcI"
                     /locus_tag="ACHKBL_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFO5817969.1"
                     /translation="MEKNSLFSQRIRLRHLHTFVAVAQQGTLGRAAETLNLSQPALSK
                     TLNELEQLTGARLFERGRQGAQLTLPGEQFLTHAVRVLDAINTAGQSLHRKEGLNNDV
                     VRVGALPTAALGILPSVIGQFHQQQKETTLQVATMSNPMILAGLKTGEIDIGIGRMSD
                     PELMTGLNYELLFLESLKLVVRPNHPLLQENVTLSRVLEWPVVVSPEGTAPRQHSDAL
                     VQSQGCKIPSGCIETLSASLSRQLTVEYDYVWFVPSGAVKDDLRHATLVALPVPGHGA
                     GEPIGILTRVDATFSSGCQLMINAIRKSMPF"
     gene            24695..26038
                     /gene="ydcJ"
                     /locus_tag="ACHKBL_001064"
     CDS             24695..26038
                     /gene="ydcJ"
                     /locus_tag="ACHKBL_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFO5817970.1"
                     /translation="MANSITADEIREQFSQAMSAMYQQEVPQYGTLLELVADVNLAVL
                     ENNPQLHEKMVNADELARLNVERHGAIRVGTAQELATLRRMFAIMGMYPVSYYDLSQA
                     GVPVHSTAFRPIDDASLARNPFRVFTSLLRLELIENEILRQKAAEILRQRDIFTPRCR
                     QLLEEYEQRGGFNETQAQEFVQEALETFRWHQSATVDEETYRALHNEHRLIADVVCFP
                     GCHINHLTPRTLDIDRVQSMMPECGIEPKILIEGPPRREVPILLRQTSFKALEETVLF
                     AGQKQGTHTARFGEIEQRGVALTPKGRQLYDDLLRNAGTGQDNLTHQMHLQETFRTFP
                     DSEFLMRQQGLAWFRYRLTPSGETHRQAIHPGDDPQPLIERGWVVAQPITYEDFLPVS
                     AAGIFQSNLGNETQARSHGNASREAFEQALGCPVLDEFQLYQEAEERSKRRCGLL"
     gene            26263..27918
                     /gene="opgD"
                     /locus_tag="ACHKBL_001065"
                     /gene_synonym="mdoD"
     CDS             26263..27918
                     /gene="opgD"
                     /locus_tag="ACHKBL_001065"
                     /gene_synonym="mdoD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein OpgD"
                     /protein_id="HFO5817971.1"
                     /translation="MDRRRFIKGSMAMAAVCGTSGIASLFSQAAFAADSDIADGQTQR
                     FDFSILQSMAHDLAQTAWRGAPRPLPDTLATMTPQAYNSIQYDAEKSLWHNVENRQLD
                     AQFFHMGMGFRRRVRMFSVDPATHLAREIHFRPELFKYNDAGVDTKQLEGQSDLGFAG
                     FRVFKAPELARRDVVSFLGASYFRAVDDTYQYGLSARGLAIDTYTDSKEEFPDFTDFW
                     FDTVKPGATTFTVYALLDSASITGAYKFTIHCEKSQVIMDVENHLYARKDIKQLGIAP
                     MTSMFSCGTNERRMCDTIHPQIHDSDRLSMWRGNGEWICRPLNNPQKLQFNAYTDNNP
                     KGFGLLQLDRDFSHYQDIMGWYNKRPSLWVEPRNKWGKGTIGLMEIPTTGETLDNIVC
                     FWQPEKAVKAGDEFAFQYRLYWSAQPPVHCPLARVMATRTGMGGFPEGWAPGEHYPEK
                     WARRFAVDFVGGDLKAAAPKGIEPVITLSSGEAKQIEILYIEPIDGYRIQFDWYPTSD
                     STDPVDMRMYLRCQGDAISETWLYQYFPPAPDKRQYVDDRVMS"
     gene            28058..28282
                     /gene="ydcH"
                     /locus_tag="ACHKBL_001066"
     CDS             28058..28282
                     /gene="ydcH"
                     /locus_tag="ACHKBL_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415943.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HFO5817972.1"
                     /translation="MFPEYRDLISRLKNENPRFMSLFDKHNKLDHEIARKEGSDGRGY
                     NAEVVRMKKQKLQLKDEMLKILQQVSVKEV"
     gene            28345..28884
                     /gene="rimL"
                     /locus_tag="ACHKBL_001067"
     CDS             28345..28884
                     /gene="rimL"
                     /locus_tag="ACHKBL_001067"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12-serine
                     acetyltransferase"
                     /protein_id="HFO5817973.1"
                     /translation="MTETIKVSESLELHAVAESHVTPLYQLICKNRTWLQQSLNWPQF
                     VQSEEDTRKTVQGNVMLHQRGYAKMFMIFKEDELIGVISFNRIEPLNKTAEIGYWLDE
                     SHQGQGIISQALQALIHHYAQSGELRRFVIKCRVDNAQSNQVALRNGFILEGCLKQAE
                     FLNDAYDDVNLYARIIDSQ"
     gene            complement(28876..29856)
                     /gene="ydcK"
                     /locus_tag="ACHKBL_001068"
     CDS             complement(28876..29856)
                     /gene="ydcK"
                     /locus_tag="ACHKBL_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcK family protein"
                     /protein_id="HFO5817974.1"
                     /translation="MRKYRLSEEQRAFSYQDDGTKKSVLLRQIIAMSDFNDVIAGTAG
                     GWIDCETVLAQEGNCWIYDQNAIAFGGAVISGNTRITGTSVLWGEVYATDNVWIDNSE
                     ISQGAYISDSVTIRDSLVCGQCRIFGHALIDQHSMIVAAQGLTPDHQLLLQIYDRARV
                     SASRIVHQAQIYGDAVVRYAFIEHRAEVFDFASVEGNEENNVWLCDCAKVYGHAQVKA
                     GIEEDAIPTIHYSSQVAEYAIVEGNCVLKHHVLIGGNAVVRGGPILLDEHVVIQGESR
                     ITGAVIIEKHVELTDHAVVEAFDGDTVHVRGPKVINGEERITRTPLAGLL"
     gene            29980..30972
                     /gene="tehA"
                     /locus_tag="ACHKBL_001069"
     CDS             29980..30972
                     /gene="tehA"
                     /locus_tag="ACHKBL_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HFO5817975.1"
                     /translation="MQSDKVLNLPAGYFGIVLGTIGMGFAWRYASQVWQVSHWLGDGL
                     VILAMIIWGLLTSAFITRLIRFPHSVLAEVRHPVLSSFVSLFPATTMLVAIGFVPWFR
                     PLAVCLFSFGVVVQLAYAAWQTAGLWRGSHPEEATTPGLYLPTVANNFISAMACGALG
                     YTDAGLVFLGAGVFSWLSLEPVILQRLRSSGELPTALRTSLGIQLAPALVACSAWLSV
                     NGGEGDTLAKMLFGYGLLQLLFMLRLMPWYLSQPFNASFWSFSFGVSALATTGLHLGS
                     GSDNGFFHTLAVPLFIFTNFIIAILLIRTFALLMQGKLLIRTERAVLMKAEDKE"
     gene            30969..31562
                     /gene="tehB"
                     /locus_tag="ACHKBL_001070"
     CDS             30969..31562
                     /gene="tehB"
                     /locus_tag="ACHKBL_001070"
                     /EC_number="2.1.1.265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0046690 - response to tellurium ion
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
                     /protein_id="HFO5817976.1"
                     /translation="MIIRDENYFTDKYELTRTHSEVLEAVKVVKPGKTLDLGCGNGRN
                     SLYLAANGYDVDAWDKNAMSIANVERIKSIENLENLHTRVVDLNNLTFDGQYDFILST
                     VVLMFLEAKTIPGLIANMQRCTKPGGYNLIVAAMDTADYPCTVGFPFAFKEGELRRYY
                     EGWEMVKYNEDVGELHRTDANGNRIKLRFATMLARKK"
BASE COUNT         7921 a         7277 c         8287 g         8255 t
ORIGIN      
        1 aaaattcatc aacaactatg cttagtgtag gcgcaacctt caactgaacg gttaaacatg
       61 ccacaatacc cgtattgaat gcttaatttt tcgctaaatc aggatattaa ctacccatgc
      121 tgggtaaata taaagccgtt ctcgcgctat tattactgat tattcttgtg ccgttgacac
      181 tgctgatgac gctcgggctg tgggttccca cgctggcggg catctggcta ccgctcggga
      241 cacgtattgc attagatgaa agcccacgca ttacgcgtaa aggtttaatc attcccgatc
      301 tccgttatct ggtgggagat tgtcagcttg cgcatatcac caacgccagc ctttcacatc
      361 ccagccgctg gttattgaac gtcggcacgg tagaacttga ttctgcttgt ctggcgaaat
      421 tgccgcagac ggagcaatcg cctgccgctc caaaaaccct cgcgcagtgg caggccatgt
      481 tgcctaacac ctggatcaat atcgataaac tgattttttc tccctggcag gaatggcagg
      541 gaaaactctc tctcgcatta acctccgata tccagcaact gcgttatcag ggcgaaaaag
      601 ttaaatttca aggccagctg aaagggcaac aacttacagt cagcgaactg gatgtcgtcg
      661 cgtttgaaaa tcagccgccg gttaaactgg tgggggaatt tactatgccg ctagtgccgg
      721 atggacttcc tgtaagtggt catgctactg cgacgttaaa cttgccgcag gagccttcac
      781 tggtggatgc cgagctggac tggcaggaaa atagcgggca attgattgtg gtggcacggg
      841 ataacggcga tccgttgctc gatctgccgt ggcaaattac tcgccaacaa ttgaccgtaa
      901 gcgatggtcg ctggagctgg ccgtatgcag gttttccttt gagtggacga ctgggtgtca
      961 aagtcgacaa ctggcaggca gggctcgaga acgctctggt cagcggacga ctgagtgtgc
     1021 tgacccaggg gcaagcgggt aagggcaacg cggtgcttaa ttttggccca ggaaaattaa
     1081 gtatggataa cagccagctg cctttgcagc tgaccggtga agcgaaacag gcggacctca
     1141 ttttatatgc ccgtttacct gcgcagctaa gcggaagtct gactgaccca acgctggcct
     1201 ttgagccagg cgcgttatta cgttcgaagg gaagagtcat cgattcgctg gacatcgatg
     1261 aaatccgctg gcctttagcg ggtgtaaaag tcacccaacg tggtgttgac ggacatttgc
     1321 aggccatttt gcaggcgcat gaaaatgaac tgggcgattt cgtgctgcat atggatggac
     1381 tggcgaatga ttttctccct gacgctggcc gctggcagtg gcgctactgg ggaaaaggca
     1441 gttttacacc gatgaatgcc acctgggatg tcgcaggaaa gggtgagtgg catgacagca
     1501 cgattacgct gaccgatctc tccaccggtt tcgaccagtt acaatacggt acgatgacgg
     1561 tagaaaagcc gcgattaatt ctcgacaagc ccgtcgtctg ggtacgtgac gcacagcatc
     1621 cctcctttag cggcgcgctg tcactggacg ccgggcaaac gctgttcact ggcggcagtg
     1681 tgttaccgcc atcaacctta aaatttagtg tcgatgggcg cgatccgacg tatttcctct
     1741 ttaaaggcga tttgcatgct ggtgagattg gcccggttcg ggtaaatggt cgctgggacg
     1801 gtattcgtct gcgcggtaac gcctggtggc ctaaacaatc actgactgta ttccagccgc
     1861 tggtgccacc cgactggaag atgaacttac gcgatggcga actgtatgct caggttgcat
     1921 tttcagctgc gcctgaacaa ggattccgcg cgggagggca cggtgtgttg aaaggcggta
     1981 gtgcctggat gccagataat caggttaacg gtgtcgattt tgtcctgcct ttccgttttg
     2041 ccgatggagc ctggcatctg gggactcgcg gccccgttac gttgcgaatt gccgaagtga
     2101 ttaatctggt gacagcgaaa aatattacgg ctgatttgca agggcgttat ccgtggagtg
     2161 aagacgaacc attgctgttg actgatgtta gcgtcgatgt gttaggcggt aacgtgctga
     2221 tgaaacaatt acgcatgccg caacatgact cggcgctgtt gcggctgaat aatctctcat
     2281 ccagcgaact ggttagcgcc gtcaatccga aacaattcgc catgtccggg gcatttagtg
     2341 gtgcattgcc gttatggcta aacaacgaaa aatggatagt gaaagacggc tggctggcaa
     2401 atagcgggcc aatgaccttg cggctggata aagacaccgc agatgcggtg gtgaaagaca
     2461 atatgaccgc gggttcagca attaactggt tgcgctatat ggaaattagc cgttcatcga
     2521 caaaaattaa tttagataat ctcggtttat taaccatgca ggccaatatt acaggtacca
     2581 gtcgcgttga tggtaaaagc ggtacggtaa atcttaatta ccatcatgaa gagaatattt
     2641 ttacgctgtg gcgcagttta cgctttggcg ataatctcca ggcatggctg gagcagaacg
     2701 cgcatctacc gggaaatgac tgtccgcaag gaaaagagtg tgaggataaa caatgaaaat
     2761 tttactggct gcgttgacgt catcttttat gctggttggc tgtacgcctc gcattgaagt
     2821 cgctgcacct aaggaaccga tcactatcaa tatgaacgtt aaaattgagc atgagatcat
     2881 catcaaggca gacaaagatg tcgaagagct gcttgagacc cgtagcgatc ttttttgagg
     2941 tgatgatgaa aagaacatta cttctttgtg catttcttgt tggcttggta agcagcaatg
     3001 taatggcatt gactctggat gaagccagaa ctcaggggcg ggtaggcgaa acattttatg
     3061 gttatctggt tgcgctgaaa acagatgctg aaactgagaa attagtaacc gacatcaatg
     3121 ccgaacgtaa agcgagctat caacaactgg caaagcagaa taatgtgtcg gtggatgata
     3181 tcgcgaaact cgccgggcaa aagcttgtag aacgggccaa acccggggaa tatgtacaag
     3241 ggataaacgg taaatgggtg cgaaaatttt aatagcaaaa ccgcaatatc gggataacgc
     3301 tacaggagaa gacgattaat ctattttccg gaaacaacgt gaatattcaa aaattcctgt
     3361 caaattgcct ttcgccctga aaaatgcata gtcaaatatc tgttttaact aattcgcgtt
     3421 gctgtacatg caacgcgaat tagttagcgg aatttacgtc gatactcgcc tggcgtcatc
     3481 ccaaagcgtt gcttaaatac cgttgaaaaa tgactctggt cggaaaatcc ccaatgaaag
     3541 ccaatgcctg ccagtttttc atcatctgcg gcatggcgaa tcgcatctgc acaaaaatcg
     3601 agacgacggt tacggatata ttgcgcgact accaaacctt tatcggcaaa ctttatcagc
     3661 gcgtagcgcc tgctgcttta gcgttcgtta gtgaaaaagg tgaaacctcg gtcggtgaat
     3721 tgcctgcgcc gggaagcctg tattttcaac gttttggcgg cttgaatatt gaacaggagg
     3781 cgttatgcca atttatcaga tagacggtct gactcagttt gtgccagaag agagttttgt
     3841 ccatccgaca gcggtattga tcggcgatgt tattctcggc aagggcgtgt acgttgggcc
     3901 aaataccagc ctgcgtggcg atttcggtcg tatcgtggtg aaagatggcg cgaacattca
     3961 ggataattgc gttatgcacg gttttcccga tcaggatact gtcgtggaag aagatgggca
     4021 tattggtcat agcgctatcc ttcacggctg cattatccac cgcaatgcat tagtgggtat
     4081 gaacgcggta gtgatggacg gtgcggtgat tggcgagaac agcattgttg gcgcttccgc
     4141 atttgtgaaa gccaaagcag aaatgcctgc taattacctg attgtcggca gcccggcaaa
     4201 agcgattcgt gaacttagtg agcaggaact ggcatggaaa aagcagggca cgcatgagta
     4261 tcaggtgctg gtgacacgca gtaagcagac gttgcatcaa gttgagccat tgcgggaagt
     4321 tgaacctggc aggcagcgcc tggtatttga tgagaatctg cgaccgaaac agtaacgtcc
     4381 tgatgctgga cgttctaatt tttagcatgt gatacctaaa ccgcaacgct gatacaggtt
     4441 gcggtttttt tattgccgga tgcggtactt gacgcgtttt gttttggtgt ctttcagggc
     4501 aatagagcaa ctcatccagt aatcttgttt acgccttcgc attatttatc tcttttcgtt
     4561 tctatactat ttttcttaat ccgttttatt acagggcagg gtgcgatgag cagcaataca
     4621 tttactctcg gtacaaaatc cgttaaccgt cttggttatg gcgcgatgca actggcaggt
     4681 cctggggttt ttggcccccc aagagatcgc cacgtcgcta taaccgtgct gcgtgaggcg
     4741 ctggcattgg gcgtcaatca tattgatacc agcgactttt atggtccgca cgtcaccaat
     4801 cagattatcc gcgaagcgct ttatccttac tctgacgacc tgacaattgt cactaaaatt
     4861 ggtgcgcggc gtggagagga cgcatcctgg ttgcccgcat tttctccggc agagctgcaa
     4921 aaagcggtgc acgataatct acgtaatctc gggctgggcg cactggatgt ggttaacctg
     4981 cgcgttatga tgggggatgg tcatggccca gcggaaggat cgattgaagc cagcctgacc
     5041 gtgctggcag agatgcaaca acaaggcctg gtaaaacata ttggtctgag caacgttaca
     5101 ccgacgcagg ttgcagaggc gcgtaagatt accgaaatcg tctgtgtgca gaacgaatac
     5161 aacattgctc atcgtgctga tgacgcaatg attgatgctt tggctcgtga cggcattgcc
     5221 tacgtgccgt tctttccgct tgggggcttt acgccgctgc aatcctccac actttcggat
     5281 gtagcagcga gcctgggcgc aacaccaatg caggtggcgc tggcgtggct gttacagcgt
     5341 tcaccgaata ttttgctgat cccagggacg tcttctgttg cgcatttacg ggagaatatg
     5401 gctgctgaaa aattgcatct ttctgaggaa gtgttgtcta cgttggacgg tatttcgcga
     5461 gaataacgaa tatacaaaag ggaaagatgc atttcccttt tgtttctttt ttaatggcat
     5521 ggagtgcata tgtttaagcc caggatttgc agctggatag gtttacttcc cctgtttatg
     5581 ctgagtcttc cagtacaggc ggaacttcga tgtgttgtaa atgcagttga tattgaacca
     5641 tttttttctg cggctaccgc tgaagataaa caacaagttg aacaagctat caacagtagc
     5701 gtgaatcttg tccccgtcgg tttatctgca tcggactgga aagtgcatcg tggcgattta
     5761 atggtagaag gtaatataga gagtaatcaa aaattgattg ttcttggcaa tttgacagtc
     5821 aaaggtaata tttccacttt ctctctttct aatccatggg ttattctcgg taacgtaacg
     5881 taacgtaacg taacgtaacg taacggcaac gaatatagtt acggattcac cactgttaat
     5941 taccggttcg ataaatgcaa gtggactggt atttatcgac tcatattacg ataatccgtc
     6001 tacgattaag ggaagtatta atgcgcgcgg gatatttatc aatgacataa ttgcacctgt
     6061 tgttgcatct tcgacaaata gtgaattcat ggtccgtgcg agtgacaaaa atgacactga
     6121 aaatgtcaaa aaagcgctga tgataataaa tcctgatgca tattattggg gcctaataaa
     6181 tgatgaagat gctctgaaag aaatttttaa gcgaagcaat attcgcatag cagggaatgt
     6241 ctgtaatcag atgaaaaaag aagcgctgtt tcgccctaag tcttctcctg aattattgca
     6301 ggaattgcaa atgctggatg aaggcaacgt tgctgcattt gaaggacgag acattgcgac
     6361 atttgatctt gccataatgc gaactctccc aaggcttaaa ggaattacag caaaacacgc
     6421 cacgaaatga tatttgggcg aagttttttc tgaggcagga aaacagttcc cgtgcgcagg
     6481 ttaacgaagc gttacgtgtt tattatgcac tagaacccga tgcgttagca caactggatg
     6541 tactggcaaa acaaccggat cgtatatggt ggagtacact ggcgaaaagc aatctcacat
     6601 ttttcaagtt cggcgcactt aacaaccgcc acacaccgcc tgcagtactg gcggcagaaa
     6661 ttgatcccga gtggtggatt gtggcgatga ataatccccg ttttccagtt gatgtattaa
     6721 aggcgaggct gaaacgtgat cctttgctgg cgttagagct tgttaaccct gaactggatt
     6781 tagtccgcca gctggcgctt aacggtaaga cgcgcgcaat acgggaacaa gcgatgagaa
     6841 aacttgatga gttgcattga ataacagcgt aatattttta actatttgat taattgggga
     6901 taatcattcc tgacagtgag ccccaatacc ttgatatatt ctgaattttt aatgaagcgg
     6961 cgtgttgcga tatctctgcc agggcaattg atgcaccata gcgcaaacag aattatcaag
     7021 gattgataat gacgctctac cagataaaac cgctctttca gtcactgtta aggccgacga
     7081 tgttttggct tcataagcgg caagtgtctg caaatcagat cacccttgcc gcgttggcac
     7141 tttctcttct caccggtttg ttgttggcgt tggtgccata tcatggcatg tttctgctat
     7201 tgcccatcgt gctttttgtc cgtatggcac tcaatgcgct ggatggcatg atggcgcgtg
     7261 agtgcaatca gcaaacacgt cttggggcga ttttgaatga aactggcgat gttatttccg
     7321 atattgcgct ttatttaccg tttttatttt taccggaaag taacgcatca ctcgtaatac
     7381 tcatgttatt ttgcaccata ttgactgagt tttgcggttt actcgcgcag acgattaacg
     7441 gtattcgcag ttatgccggt ccatttggca aaagcgatcg cgcgttaata tttgggttgt
     7501 gggtgctggc cgttgccatt tatccacagt ggatgcaatg gaataatctt ttatggagca
     7561 ttgcttcaat tctgcttctc tggactgcga ttaatcgttg tcggagtgtg ctcctgatga
     7621 acgctgaaaa ataatgctgg aaaaatctct ggcaacactt ttcgcgttgt taattttagc
     7681 aacgctgata aaccgttttc tggtatggcg gttaccggag agaaaagggg atgaagttac
     7741 attacgtatt cgtacctggt ggggcatcgt catttgtttt tcactggtga tttcaggtcc
     7801 acgctgggtg acgttgacgt tttttgcgct gataagtttt ctggcattga aagaatattg
     7861 tacgcttata tccgtacatt ttccgcgttg gttatattgg gttattcctc ttaactattt
     7921 gcttatcggt ttaaattgct ttgagctgtt tctgttattt atacctttgt ttgggtttct
     7981 gatattagcc acctggcgag tttttgtggg tgaaccctcc ggttttctgc ataccgtgag
     8041 cgccattttt tgtggctgga taatgaccgt tttcgccttg agtcatgccg cctggttatt
     8101 aatgttgcca accacaaata tccagggcga ggcgttactg gtgttatttc ttttggcatt
     8161 aaccgagtca aacgatattg cacagtattt atgggggaaa tcctgcggca gaagaaaagt
     8221 ggtccccaaa gtcagcccgg gaaaaacgct ggaaggtctg gtggggggcg taatcactac
     8281 catgattgtg tcactgatta ttggaccgtt actgacaccg ctagatacat tacaggcatt
     8341 attagcgggt ttgttaattg gtattagtgg ttttgcggcg atgtagtgat gtcagccatc
     8401 aaacgagatg ttggtgttaa agatagtgga aaactattac caggacatgg cggactcctt
     8461 gacaggattg actcattaat tttcaccgct ccggtatttt tttattttat tcgctattgc
     8521 tgttactgaa ggaaaataga gagaatggaa aattcacgca tccctgggga acattttttt
     8581 accaccagtg ataatacagc gttgttttat cggcactggc ccactttaca gccaggggcg
     8641 aaaaaggtca tcgtcttatt tcatcgcggt catgaacatt ctggtcgtct acaacatctc
     8701 gttgatgaac tggcgatgcc agatactgct ttttatgcat gggatgcccg agggcatgga
     8761 caaacttcgg ggccgcgtgg ttatagtccg tctcttgcga gttcagtgca agatgtcgat
     8821 gaatttgtcc gttttgctgc cagcgacagc caggtcggac tggaagaggt ggttgtgatc
     8881 gcgcaaagcg tcggcgcagt gatggttgct acatgggttc atgattatgc gcctgctatt
     8941 cgcgggctgg tgctggcttc tccggccttt aaggttaaat tgtatgtgcc gctggcacgt
     9001 cctgcgctgg cgttatggca tcgtctgcgt ggtctgtttt ttattaattc ctatgtgaaa
     9061 ggacgctatt tgacccacga tcggcaacgg gtggctagtt tcaataatga tccgctgatc
     9121 acaagggcga ttgccgtcaa tatcttgcta gatctctata aaacgtctga acgtattatt
     9181 agcgatgcgg cggcgattac gctccccacg caacttctga tatcaggcga tgactatgtg
     9241 gtgcaccgcc aaccgcagat tgatttttat cagcgtttac gtagccctct gaaagagctg
     9301 catctgctgc caggctttta tcacgacacg ttgggtgaag agaacagggc gctggtattt
     9361 gaaaaaatgc aaagctttat tagtcactta tatgctaaca aatcgcaaaa attagattat
     9421 cagcatgaag actgcacagg accatcagcg gatcgatggc ggctactttc tggtggaccc
     9481 gtgccattat cgccggttga tttggcgtat cgctttatgc gtaaagcgat gaaattgttc
     9541 ggggcgcact ctgcgggcct gcatctcgga atgagcaccg gctttgattc aggcagttcg
     9601 ctggattatg tctatcaaaa tcaaccgcaa ggtagtaacg cattcgggcg tttagtcgac
     9661 aaaatctacc tgaacagtgt tggctggcgc ggtattcgcc agcgcaaaac ccatttacaa
     9721 atgctgatta aacaagccgt tgccgatctc cacgctaaag gtttagccgt ccgcgtggtt
     9781 gacattgccg cagggcatgg acgctatgta ctggatgcgc tggcgaacga gcctgccgta
     9841 agcgatattt tcttacgtga ttacagtgag ttaaatgttg cacaggggca agagatgatt
     9901 gcccaacggg gaatgtctgg gcgggtgcgt tttgaacagg gcgatgcgtt taacccggag
     9961 gagctcagcg cgttaactcc gcggcctacg ctggcgattg tctctggact gtatgagctt
    10021 tttcccgaaa atgagcaggt aaaaaactca ctcgcaggtc ttgccaatgc catcgatccg
    10081 ggtggcattc tcatctacac cgggcagccg tggcacccac aactggagct gattgccggg
    10141 gtgttaacca gtcataaaga tggtaaaccg tgggtaatgc gcgtgcgttc tcaaggggag
    10201 atggattcgc tcgtgcatga tgccggattc gataaatgca cacaacggat tgatgagtgg
    10261 ggcattttta cggtttcgat ggcggtgcgt cgtggtaact gaacgccgga acgtattgct
    10321 acaaggcgct ggctggttat tgttgctggc cccgtttttc ttcttcacat atggatttct
    10381 taatcagttc accgcgactc aggatcttaa caaccatgat atccccagtc agttattcgg
    10441 ttgggaaacg gcgatccctt ttcttccctg gactattgtt ccttactgga gtctggatct
    10501 tttatatgga ttttcgctgt ttgtttgtag ctcgacattt gagcagcgcc gtctggtcca
    10561 ccggcttatt ctggcaacgg taatggcctg ctgcggtttt tttctctatc cgctgaagtt
    10621 tagttttatc cgtcctgaag tgagtggggt gacaggatgg ttattttcgc aacttgaatt
    10681 gtttgatctg ccttataacc agtctccttc gctgcatatt attctctgct ggctactttg
    10741 gcgtcacttt cgtcagcatc tggctgtgag gtggcgtaaa gtctgcggcg gatggttttt
    10801 actcatcgcc atttcgacgc tgacgacctg gcagcatcat tttattgatg tcatcacagg
    10861 gctggcggta ggtatgttaa ttgactggat ggtgcccgtc gaccgtcgtt ggaattatca
    10921 gaaacctgat caacgtcgaa tcaaaatagc actgccatat gtcgtaggcg cgtgctcgtg
    10981 cattgtgttg atggagctaa tgataatgct tcagttatgg tggtcagtct ggttatgttg
    11041 gccagtatta tcgctattca tcattggccg tgggtacggt gggcttggcg cgataacaac
    11101 agggaaagat agttagggga aactcccgcc cgccgtttac tggctgacat tgccctggcg
    11161 catcgggatg tggctgtcta tgcgttggtt ttgtcttcgc ctggagtcgg tgagcaaaat
    11221 tactgctggt gtttatttag gggcgtttcc acaacatatt ccggcacaga atgctgttct
    11281 ggacgtcacc tttgaattcc ctcgcggacg agccacaaaa gatcaactct atttttgtgt
    11341 accgatgctg gatctggtgg ttccggaaga gggggagctc cgacaggccg tggcgatgct
    11401 ggaaacatta cgcgaagagc aaggcagcgt tctggtccat tgcgcgttgg gattatcgcg
    11461 cagtgcgctg gtggtggcgg catggctgtt atgttacgga cattgtaaaa ccgttgatga
    11521 agcgattagt tatattcgag ccagacgctc gcggattgtg cttaaggaag agcacaaagc
    11581 gatgctgaaa ttatgggaaa acaggtaagc ggattgagat gtggactgaa tatctacagt
    11641 ccacatcaag atcgtgtcca gttattcagc aacaatactg tcgatggctg ctttcgcgtc
    11701 agactgcgct ttcgctgcca tttcaggacc gtatgcgatc ccttcggcga agacaaattt
    11761 cacatcggta atgccgataa agcccaggaa tgtggacaga tacggcgtca ccagatccgt
    11821 tgggccatct ttatggatcc caccgcggct ggtgataacg atggcttttt taccggtgac
    11881 caggccttcc ggaccgttct ccgtatagcg gaaagtaacg cctgcgcgtg caaccaggtc
    11941 aaaatagttt ttcaactgag tcgagatgtt gaagttatac atcggtgccg caataacgat
    12001 aacgtcgtgg gctttcaact cggcaatcag ctcatcggaa agtgccagag cttcctgctg
    12061 acgcggagtc agcggtgcat cgctcggacg cagcgcacca accagttcgc catccagcac
    12121 cggaatcgga tttgcagcca ggtcgcgaac ggtgatttca tcagcggagt gcttttcgcg
    12181 ccattgttca acaaaataat cggacaactg attagactga gagtaccctg ccaggatgct
    12241 ggatttaaga actaatacct tgctcatggt gtttccttat agatgtttga atgggcgatg
    12301 ccccgttgct tgttgacact ttattcacaa tcctgccaca gagatagcgc aataaatcga
    12361 agcctatgtt cgaatttatt gaacaacgca tagaaagccg cgatgtggta ctctatatct
    12421 atcatttaaa agaaaattaa tcaggcagac tactgcccac taacgttatg acagaacaac
    12481 aaaaattgac ctttacggcc ttgcagcagc ggctggattc gctgatgctg cgcgacagac
    12541 tgcgtttttc tcgccgtctg cacggcgtga agaaggttaa aaatcctgat gcacaacagg
    12601 ccattttcca ggagatggcg aaagagattg accatgcggc agggaaagtc ctgctgcgtg
    12661 aagcggcacg accggaaatc acttatccgg acaatttgcc ggttagtcag aaaaagcagg
    12721 acatactcga agcgattcgt gatcaccagg tggtgatcgt cgccggggaa accggttccg
    12781 gtaaaacgac tcagctaccg aaaatctgta tggaactggg gcgcgggatt aaagggctga
    12841 tcggtcatac ccaaccgcgt cgcctggcgg cacgaacggt ggcgaaccgt attgcggaag
    12901 agctgaaaac ggagccgggc ggttgcatcg gttacaaagt gcgtttcagt gatcacgtta
    12961 gtgataacac gatggtcaag ctgatgaccg acggtatcct gctggcggag atccagcaag
    13021 accgcctgct gatgcagtac gacactatca ttattgacga agcgcacgaa cgtagcctga
    13081 atatcgattt tctgctcggc tatttgaaag agttgctacc gcggcgtcct gacctaaaaa
    13141 tcattatcac ttccgcgact atcgacccgg aacgcttttc gcgccacttt aataatgcgc
    13201 cgattatcga agtctccggt cggacctatc cggtggaagt gcgctatcga ccgattgttg
    13261 aagaagccga tgacaccgag cgcgatcagc tgcaggcgat ttttgacgcc gtagacgaac
    13321 tgagccagga aagccctggc gacattctga tctttatgag tggtgagcgg gaaattcgcg
    13381 ataccgccga tgcgctgaac aagctgaact tacgccatac cgaaatcttg ccgctttatg
    13441 cgcggctttc gaacagcgaa cagaaccgcg tgttccagtc gcacagtgga cggcgcattg
    13501 tgctggcgac caacgtcgcg gaaacgtcgc tgaccgtacc gggtattaaa tacgttatcg
    13561 acccaggtac ggcgcgtatc agccgctaca gctatcgcac taaagtgcag cgtttgccga
    13621 ttgagccgat ttctcaggcg tctgccaatc agcgtaaagg ccgctgtggt cgtgtgtccg
    13681 aagggatctg tattcgtctc tattccgaag acgatttcct ctcgcgcccg gagtttaccg
    13741 atccggagat tctgcgtact aacctggcct cggttatttt gcagatgacc gcgctggggt
    13801 tgggcgatat cgccgcgttc ccatttgtcg aagcaccgga taaacgtaat atccaggatg
    13861 gcgtgcgtct gctcgaagag ctgggcgcga tcaccactga tgaacaggcc agcgcctata
    13921 aactgacgcc gctcggtcgc cagctttcgc aattgcctgt cgatcctcgt ctggcgcgta
    13981 tggtgctgga agcgcaaaaa catggctgcg tgcgtgaggc gatgattatc acgtccgcgc
    14041 tctccattca ggatccgcgc gagcgtccga tggataaaca gcaggcatca gacgaaaaac
    14101 atcgccgctt ccacgacaaa gaatccgact tcctcgcgtt tgtaaatctg tggaattatc
    14161 tcggcgagca gcaaaaggcg ctttcttcta acgccttccg tcgcctgtgt cgtaccgatt
    14221 atctcaacta tctgcgcgtg cgcgaatggc aggatatcta cacccagttg cgccaggtgg
    14281 tgaaagaact tggcattccg gttaacagcg aaccggcgga gtatcgcgaa attcacattg
    14341 cgttgctgac gggtttgctt tcccatatcg gcatgaaaga tgccgataaa caagaatata
    14401 ccggcgcacg taacgcgcgt ttttccatct ttcccggttc tggtttattc aaaaagccgc
    14461 cgaaatgggt aatggtggcg gaactggtag aaaccagccg cctgtggggg cgcattgctg
    14521 cgcgtatcga ccctgaatgg gtagaacccg ttgctcagca tttgattaaa cgcacctaca
    14581 gcgaaccgca ctgggaacgg gcgcagggcg cggtgatggc aacggaaaaa gtcactgttt
    14641 atggtttgcc gattgttgct gcgcgtaagg tcaactacag ccagatcgat ccggcgttat
    14701 gtcgtgaact ctttattcgc cacgctctgg tggaaggtga ctggcagacg cgtcacgcat
    14761 tcttccgtga aaacctgaaa ctgcgggccg aagtggaaga gctggaacac aaatcacgtc
    14821 gccgcgatat tctggttgat gacgaaacgc tgtttgagtt ctacgaccag cgcatcagcc
    14881 acgatgtaat ctccgctcgt cacttcgata gctggtggaa aaaagtcagc cgcgaaacgc
    14941 ctgatttgct caactttgaa aagagcatgt tgatcaaaga aggggcggaa aaaatcagca
    15001 agctggatta tccgaacttc tggcatcagg gcaatctcaa gctgcgtttg agctatcagt
    15061 ttgagccagg cgcggacgcg gacggtgtga ccgtgcatat tccgctgccg ctgcttaatc
    15121 aggtagaaga gagcgggttt gaatggcaaa tccccggtct gcgccgcgaa ctggtgattg
    15181 ctctgattaa atcgttgccg aaaccagtac gccgtaattt tgtgcccgcg ccaaactacg
    15241 ccgaagcgtt tctaggccgc gtcacaccgt tggaattgtc gttgctcgac agtcttgaac
    15301 gagagctacg gcggatgact ggcgtcaccg ttgaccgcga agactggcac tgggatcagg
    15361 tgcccgatca cctgaaaatc accttccgtg tggtggatga caaaaacaag aagctaaaag
    15421 aagggcgctc attacaggat ctgaaagatg ctctgaaagg caaagtgcag gaaacgctgt
    15481 ctgcggtggc ggatgacggt atcgagcaga gcggcttaca tatctggagc tttgggcaac
    15541 tgccggaaag ctacgaacag aagcgtggca actacaaagt gaaggcgtgg ccagcgttgg
    15601 tggatgagcg cgacagcgtg gcgatcaaac tgtttgataa cccgctggaa caaaagcaag
    15661 cgatgtggaa cggtcttcgt cgtttactgc tgctgaatat tccatcgccg atcaagtatt
    15721 tgcatgaaaa gttaccgaac aaagccaagc tggggctgta ctttaacccg tatggcaaag
    15781 tgctggagct gatcgacgac tgtatctcct gcggggtgga tcaactgatc gacgccaatg
    15841 gaggcccggt ctggacggaa gaaggctttg ctgcgctgca tgagaaagtc cgcgctgaac
    15901 tgaacgatac ggtggtggat attgcgaagc aggtcgagca aatccttacg gcagtgttca
    15961 atatcaacaa acgcctgaaa gggcgggtgg atatgactat ggcgctgggg ctttctgaca
    16021 tcaaagcgca gatgggcggg ctggtgtatc gcggttttgt cactggtaac ggttttaaac
    16081 ggctgggcga cacgctgcgt tatttgcagg cgattgaaaa acgactggaa aaactggcgg
    16141 ttgatccgca tcgtgaccgt gctcagatgc tgaaagtcga aaatgtccag caggcgtggc
    16201 agcaatggat caacaaactg ccgcccgcac gtcgtgagga tgaagacgtg aaagagatcc
    16261 gttggatgat agaagagttg cgcgttagtt acttcgctca acaacttggt acgccttatc
    16321 cgatttcaga taagcgtatt ttgcaggcga tggaacagat tagcggttaa cgcggctatt
    16381 tgcctgataa atctaaaacc cggtaagcat ttagcgccgg gttttttatg aattctaaaa
    16441 cggtaagggc aaaaattcag gaattcagaa aaatacaatt ctcagctgca agatgaataa
    16501 tgtttatcta cagcatttcc ttaaaagata tgtcaggctt gcggagtggc ggttaaggaa
    16561 atacgacttc ctcctttcag agtgctccgc ttctcactat tatctcacgc agtattctta
    16621 agggaacgat aaggaggaac catgaacatt accccgtttc cgacgctttc gccggcaacc
    16681 atagatgcca taaatgttat cggtcagtgg ctggcgcagg atgatttctc cggtgagatg
    16741 ccgtatcagg ccgattgcgt gatccttgca ggcaatgcgg ttatgccgac tatcgacgcg
    16801 gcatgtaaga ttgctcgcga tcagcaaatt cctctactga ttagtggtgg tatcggtcac
    16861 tcgacaactt ttttgtatag cgccatcgca cagcatccgc actacaacac tatccgcacc
    16921 actggcagag cagaagcgac catcctggcg gatatcgctc atcagttctg gcacattccg
    16981 catgaaaaaa tctggattga agaccagtca acaaactgcg gtgaaaacgc acgctttagc
    17041 atcgcgctat tgaatcaggc cgtagaacga gttcatacgg ctatcgttgt tcaggacccc
    17101 accatgcagc ggcgcacgat ggcgacgttc cgccgtatga ctggggacaa tcccgatgca
    17161 ccacgctggt taagttatcc cggattcgtt cctcagttag gaaataacgc agacagtgta
    17221 atctttgtta atccgttgca aggattatgg ccggttgagc gttatctctc actactcact
    17281 ggcgagctgc cgcgtttacg cgatgatagc gatggctacg gtccccgcgg gcgagatttt
    17341 atcgttcacg ttgattttcc ggcagaagtc atccatgcat ggcaaacgct gaaacacgat
    17401 gcggtgctaa tcgaggcgat ggaaagtcgc tcgttacgtt aaaaattgcc cgtttgtgaa
    17461 ccacttgttt gcaaacgggc ataactcctg ccttttattt ctgcctctta ttccttttat
    17521 acttgttttt atgaagccct tcacagaatt gtcctttcat gatgccgtct ctatgatgat
    17581 tgatgttaat taacaataca tttaccaaaa acaaacatat aaatcacagg agtcgctcat
    17641 gtcagtaccc gttcaacatc ctatgtatat tgatggacag tttgttacct ggcgtggaga
    17701 cgcatggatt gatgtggtaa accctgctac agaggctgtc atttcccgca ttcccgatgg
    17761 tcaggccgag gatgcccgta aggcaatcga tgcagcagaa cgtgcacaac cagaatggga
    17821 agcgttgcct gctattgaac gcgccagttg gttgcgcaaa atctccgccg ggatccgcga
    17881 acgcgccagt gaaatcagta cgctgattgt tgaagaaggg ggtaagatcc agcagctggc
    17941 tgaagtcgaa gtggctttta ctgcggacta tatcgattac atggcggagt gggcacggcg
    18001 ttatgaaggc gagattattc aaagcgatcg tccaggagaa aatattcttc tgtttaaacg
    18061 tgcgcttggc gtgactaccg gcattctgcc gtggaacttc ccgttcttcc tcattgcccg
    18121 caaaatggct cccgctcttt tgaccggtaa taccatcgtc attaaaccca gtgaatttac
    18181 gccaaacaat gcgattgcat tcgccaaaat tgtcgatgaa ataggccttc cgcgcggcgt
    18241 gtttaacctt gttctggggc gtggtgaaac cgttgggcaa gaactggcgg gtaacccaaa
    18301 ggtcgcaatg gtcagtatga caggcagcgt ctctgcaggt gaaaagatta tggcgactgc
    18361 ggcgaaaaac atcaccaaag tgtgcctgga attggggggt aaagcaccgg ctatcgtaat
    18421 ggacgatgcc gatcttgaac tggcagtcaa agccatcgtt gattcacgcg tcattaatag
    18481 tgggcaagtg tgtaactgtg cagaacgtgt ttatgtacag aaaggcattt atgatcagtt
    18541 tgtcaatcgg ctgggtgaag cgatgcaggc ggttcaattt ggtaaccccg ctgaacgcaa
    18601 cgacattgcg atggggccgt tgattaacgc cgcggcgctg gaaagggtcg agcaaaaagt
    18661 ggcgcgcgca gtagaagaag gggcgagagt ggcgttgggt ggcaaagcgg tagaggggaa
    18721 aggatattat tatccgccga cattgctgct ggatgttcgc caggaaatgt cgattatgca
    18781 tgaggaaacc tttggcccgg ttctgccggt ggtcgcattt gacacgctgg aagaggctat
    18841 ctcaatggcg aatgatagtg attacggcct gacctcatca atctataccc aaaatctgaa
    18901 cgtcgcgatg aaagccatta aagggctgaa gtttggtgaa acttacatca accgtgaaaa
    18961 cttcgaagct atgcaaggat tccacgccgg atggcgtaaa tctggtattg gcggcgcaga
    19021 tggcaaacat ggcttgcatg aatatctgca gacccaggtg gtttatttac agtcttaatg
    19081 agtggaagag gcggaggttt ttcctccgcc tgtgtgcgtc agagtttagc gaatttttcg
    19141 agggtgcgaa tgagttgtgt gacgaagcca tattcgttat cgtaccaggc gaccgttttc
    19201 accagttgta aatcgcccac ggcggtaatt tccgtttgcg tggcatcaaa caccgacccg
    19261 aaatggctgc caatgatatc ggaagagact atttcttcat cggtataacc aaatgactcg
    19321 ttattggtgg ttgcttgttt aagtgcgtta ttcacctctt cggcagtcac tttttttgcg
    19381 agaatggaca ccagctcagt gaccgaacct gttttcaccg gcacgcgttg cgcatgacct
    19441 ttcagtttgc cgctcagttc cgggatcacc agaccaatgg cttttgccgc acctgtagtg
    19501 tggggaatga tattttctgc cgctgcgcgt gaagctcgta aatccttccc acgcggacca
    19561 tccaccagtg actgggtgcc ggtataggca tgaatggtcg tcatcgtgcc gacttctatc
    19621 ccgaaactgt catgcaaggc tttggccatc ggcgcaaggc agttagtggt gcatgacgcc
    19681 acggaaacaa tggtgtcgtt gccatccaga gtgtcgtcat tgacgttata aacgatagtt
    19741 ttcatttcac cggcaggggc ggaaatcaac accttcttcg caccagcatc aagatgcgcc
    19801 tgcgatttct cggcggaggt ataaaagcca gtacattcga caataatttc tgcccctttc
    19861 gctttccacg gaatattttt agcctctttt tcggcgtaaa ccgcgatgct ttttccatca
    19921 acgataagtg aatcttccgt aaaatcaacg ctccagggga acggtccgta gtttgaatca
    19981 tgtttcagca ggtaggcgag aatttttggg gaagtgagat cattaatagc gacaacgtct
    20041 atgttgcttt tgacttcaag taatcgacgc aacaccagtc gaccgatacg accaaaacca
    20101 ttaataccaa ctttactcat ggtcttctcc tgtcaggaac gttcggatga aaattgatcc
    20161 tttccaggct tagaccagga tggcgggatg ggcaatctac attcttgcag tgaaacgtaa
    20221 cgtactgaaa acgggtgaac aatatttaat gaaattttaa gaaaagcccg ttatgttaac
    20281 ggcaaattat gttaaagcag gaaatgttat gggaaataaa tactcaaggt tacaaatcag
    20341 cattcactgg ctggtctttt tgctggttat cgtagcgtat tgcgcaatgg agtttcgtgg
    20401 tttcttccca cgtagcgatc ggcccctcat caacatgatt catgtttcct gtggcatctc
    20461 aattctcgta ctgatggtgg ttcgtctttt gttaagactg aaatacccaa ccccgccgat
    20521 tatacctaag ccaaaaccga tgatgacggg actggcgcat ttgggccatt tggtgattta
    20581 tctcctgttt attgcgctgc cagtgattgg tttggtgatg atgtataacc ggggcaaccc
    20641 gtggtttgcg tttggtttga cgatgcctta cgcttcagag gccaatttcg aacgggtaga
    20701 tagcttaaag tcgtggcatg aaatgctggc gaatctggga tattttgtca tcgggttgca
    20761 cgctgcggct gcactggcac accactattt ctggaaggac aacacacttc tacgcatgat
    20821 gccgcgtaaa cgttcctgaa ggatatttaa agaaaacgcc tgtactaaaa ccgacccgtg
    20881 gtacaggcga agaatacggg tctacatcgg aagcgcctat tatatttatt tgtatgacaa
    20941 ataaaaccat aatccttgcc catacgtcca tctggcttat ttttaatcaa ttcacccgat
    21001 ctttgatctc atcaacggta tcaaaataaa ccagcgtata acgttaaaat cgaacataag
    21061 attcgataaa ccatggaggt tatatgaaaa aactggcact tatcttggtt ataggaacgc
    21121 ttgtttcctt ttatgccgat gccgggcgca aaccctgttc tggttcgaaa ggggggatct
    21181 cacactgtac ggcaggcggc aaatttgttt gtaatgatgg ttctattagt gcatcgaaaa
    21241 aaacatgcat taactgaagt gtaaaagggg tgccatgaga aaatgaatct gctagtcaga
    21301 tgcgcgggga aaatccccgc gcttgccctt acctggacgt gcaggccatg agcgcagcaa
    21361 cctccttatc accgtcccgg aaccgcagtt cgtagagcgt ttgtcgggtc aggagtgtga
    21421 atgtcagaat cgtaatgcag ataatgagca gacacaccac cagagggttg cgcttcatag
    21481 cctttctcct tgccggatgg cgggtaagag gctaagatct gaattgctgt gttcattcgt
    21541 tggcctcggt tgatagaaat atcggtcggg gccttcgtct ttctgattcc cggttagcct
    21601 gaaaacagaa agtctcaggc acccgtgagc atcctatgtg gtttccttag ggacgaaaat
    21661 aatcacctga cgaaattgcg tgctgttttc cagaattttt cgtcattcgg gttagccagt
    21721 ttaatcattc gttactctct tcattccaat ggcattattc ttctctgcca taattgttgt
    21781 aaaaatgtga cgcaaagagg tttttggtca taagtaatta ccgtcaagtg ccgatgactt
    21841 tctatcagga gtaaacctgg acgagagaca acggtaatga atacaactcc ctcacagcga
    21901 ttaggttttt tgcatcacat caggctggtt ccgttatttg cctgcattct tggcggtatc
    21961 ttagttctat tcgcattaag ttctgctctg gctggctatt tcctctggca ggccgatcgc
    22021 gatcagcgtg atgttactgc ggagattgag attcggaccg ggttagcgaa cagttcagat
    22081 tttttgcgtt cagcccggat caatatgatt caggccgggg cagcgagtcg tattgcggaa
    22141 atggaagcaa tgaagcgaaa tattgcgcaa gccgaatcgg agattaaaca gtcgcagcaa
    22201 ggttatcgtg cttatcagaa tcggtcggtg aaaacacctg ctgatgaagc cctcgacact
    22261 gaattaaatc aacgcttcca ggcttatatc acgggtatgc aacctatgtt gaaatatgcc
    22321 aaaaatggca tgtttgaagc gattatcaat catgaaagtg agcagatccg accgctggat
    22381 aatgcttata ccgatatttt gagcaaagcc gttaagatac gtagcaccag agccaaccaa
    22441 ctggcggaac tggcccatca gcgcacccgc ctgggtggga tgttcatgat tggcgcgttt
    22501 gtgcttgccc tggtcatgac gctgataaca tttatggtgc tacgtcggat cgtcattcgt
    22561 ccactgcaac atgccgcaca acggattgaa aaaatcgcca gtggcgatct gacgatgaat
    22621 gatgaaccgg cgggtcgtaa tgaaatcggt cgcttaagtc gtcatttaca gcaaatgcag
    22681 cattcactgg ggatgacagt agggactgtt cgacagggtg cggaagagat ttatcgtggc
    22741 accagcgaaa tttccgctgg caatgcggac ctttcatctc gcaccgaaga acaagcggcg
    22801 gcaatcgaac aaactgccgc cagcatggag caactcactg cgacggtgaa acagaatgcg
    22861 gataacgcgc atcatgccag caaactggcg caagaggctt ctattaaagc cagcgatggc
    22921 gggcagacgg tttccggtgt agtaaaaacg atgggcgcta tctccacgag ttcgaagaaa
    22981 atttctgaga tcaccgccgt catcaacagt attgctttcc agacgaatat tctggcactg
    23041 aatgctgccg ttgaagccgc gcgagcgggt gagcaagggc gtggatttgc cgttgtcgcc
    23101 agcgaagtac ggacactcgc aagccgcagc gctcaggcgg cgaaagagat tgaaggcttg
    23161 atcagtgaat cagtcaggtt aattgacctg ggatcggatg aggtggccac ggccgggaaa
    23221 accatgagca ctattgttga tgccgtcgcg agtgtcacac atatcatgca ggaaattgcc
    23281 gccgcctcgg atgagcaaag taggggtata acgcaggtta gccaggcgat atctgaaatg
    23341 gataaggtga cgcaacagaa tgcttctctg gtagaagagg cctcagcagc ggcagtgtcc
    23401 cttgaagaac aggcggcacg attaactgag gcggtggacg tattccgtct gaacaaacat
    23461 tctgtgtcgg cagaacctcg cggagctggt gaaccagtta gtttcgctac agtgtgaaaa
    23521 tgttaaagga gggatcgaca gatcccttca cctttcagaa cggcattgat tttcgaatag
    23581 cgttaatcat caactggcaa ccagaagaga acgtcgcatc tacgcgggtc agtattccaa
    23641 tcggttcgcc tgcaccatga cccggaacag gcagggccac cagcgtggca tgacgcaggt
    23701 cgtcttttac agcgccagaa gggacaaacc acacgtaatc gtattcaacc gtaagttgac
    23761 gagatagcga agcagacagc gtttcgatac aacccgaagg aattttacat ccctggctct
    23821 gcactaatgc atctgaatgc tggcgtggcg cagtgccttc tggtgataca acgaccggcc
    23881 attccagcac ccggcttagc gttacgttct cctgaagtag cggatgatta gggcggacaa
    23941 ccagcttcag cgattcaaga aacagcagtt cgtaattaag tccggtcatc agttcaggat
    24001 ctgacatccg accaatgccg atatcgattt ccccggtttt taaacccgca agaatcatag
    24061 ggttactcat tgtcgcaact tgcaaggtcg tctctttttg ttgctgatga aactgaccta
    24121 taaccgaagg taatatcccc agtgccgcag tcggtagtgc accaaccctg acgacatcat
    24181 tattaagacc ttctttacga tgaagcgact gtccagcagt gttgatggcg tcaagcactc
    24241 tgactgcatg cgttaaaaat tgttcgccgg gtaaggtaag ttgcgccccc tgacgaccac
    24301 gctcaaacaa gcgagcgccc gtcagctgct ccagttcatt caatgtctta gagagcgcag
    24361 gttgactcaa attaagggtt tcagccgcgc gccccaaagt tccttgttgt gcgacagcta
    24421 cgaatgtatg aaggtggcgc aaacggatgc gctgactaaa cagactattt ttttccataa
    24481 gcgatgttaa aaacgaagcg gtgtcgctga caagtgaagt tgtttgatta tgataacttg
    24541 attgcaaaat attattaaca attaaagcaa ttatgttaca gcgaaatgga taatattgat
    24601 gttttcgcgg cgagatcaca gtttgcaaat tcttcccgcc agagtgaatg cggttaccta
    24661 cactccagat tactgaccac tggaggcaga cattatggcg aacagcatca cggcggatga
    24721 gattcgggaa cagttttcgc aggcaatgtc agccatgtac cagcaagaag ttccgcagta
    24781 cggtacgctg ctggaactgg tagctgatgt gaatctggct gtgctggaaa acaatcctca
    24841 actgcacgaa aaaatggtaa atgcagacga gctggcgcga ctgaatgttg aacgtcatgg
    24901 ggcgattcgt gttgggactg cacaagagct tgctactctt cggcggatgt ttgccattat
    24961 ggggatgtac ccggtgagct attacgatct ctcgcaggcg ggggtgccgg tacattccac
    25021 agcatttcgg cccattgatg atgcttctct ggcgcgtaat cccttccgcg tttttacttc
    25081 attactccgc cttgagctta tcgagaacga aattttgcgc cagaaagcgg cggagattct
    25141 gcgtcagcgc gatatcttca ccccacgttg tcgacaactg ttagaggaat atgagcagcg
    25201 gggcggtttt aacgaaacac aggcacagga gtttgtgcag gaagccctgg aaacgtttcg
    25261 ttggcaccag tcagcaacgg tagatgaaga aacctatcgc gccttgcaca acgaacatcg
    25321 gttgattgct gatgtggtct gttttcctgg atgccatatc aaccacctga cgccacgcac
    25381 gctggatatt gaccgggtgc agtcgatgat gcctgaatgc ggaattgaac ccaaaattct
    25441 gatcgaaggg ccgccgcgcc gcgaggtacc gattttacta cgccagacta gctttaaagc
    25501 actggaagag acggtgttgt ttgcagggca gaaacagggc acgcataccg cgcgctttgg
    25561 tgaaatagaa cagcgtggcg tggcattaac gccgaaagga cgacaactgt atgatgatct
    25621 actgcggaac gctggaaccg ggcaggataa tctcactcac caaatgcatt tacaggaaac
    25681 cttccgcact tttcctgaca gtgagttttt aatgcgtcag caagggttgg catggttccg
    25741 gtaccgtctg acgccttcag gtgagacgca tcgtcaggcg attcatcccg gagacgatcc
    25801 acagccctta attgaacgtg gttgggtagt ggcgcaaccc attacctatg aagatttctt
    25861 gcctgttagc gcggcgggga tcttccagtc aaatctgggt aatgaaacgc aggcacgcag
    25921 tcacggtaat gccagtcgcg aagcatttga gcaggcgttg ggctgcccgg ttttggatga
    25981 gttccagctt tatcaggaag cggaagaacg cagtaaacgt cgctgtggtt tgctttaaat
    26041 tctgaccatc cgcctttgca aaaatttgcc tgattttaca aacgaatcag gctcatccca
    26101 tcgacataaa aaaattgccg atttatgcat attctctcag ttcaacaatt ggattattaa
    26161 taaatattgt ctagagtgag cggtcataaa taagcacttt cttgccgctg aaaacgatca
    26221 gcgcgggacc attcacaaca ccagaaggac tcactttcag gtatggatcg tagacgattt
    26281 attaaaggtt caatggctat ggccgccgtg tgcggtacca gcggcattgc ttctcttttt
    26341 tctcaggcgg cattcgcggc agattctgat attgccgacg ggcaaaccca acgttttgac
    26401 ttctccattc tacagtcaat ggcgcacgac ttagcgcaaa cagcgtggcg tggtgcgccg
    26461 cgtccgttac ctgacactct ggcgacaatg acgccgcagg cttataacag tattcaatac
    26521 gacgccgaaa aatcgctctg gcataacgtt gagaaccgtc aactggacgc tcagttcttc
    26581 catatgggaa tgggattccg tcgccgcgtt cgtatgtttt ctgtagatcc agcaacacat
    26641 ctggcgcgtg aaattcactt tcgcccggag ttgttcaaat acaacgatgc gggtgttgat
    26701 accaaacaat tagaagggca aagtgatctc ggttttgccg gttttcgcgt gtttaaagcc
    26761 cccgaactgg cgcgccgtga tgtcgtatca ttcctcggcg cgagttattt ccgcgcggtt
    26821 gatgatacat atcaatacgg tttgtcggcc cgcggcctgg cgatcgacac ttacaccgac
    26881 agtaaagaag agttccccga ctttactgac ttctggtttg atacggtaaa accgggggca
    26941 accaccttta ccgtttatgc gttgctcgat agcgccagca ttactggtgc ctataagttc
    27001 actatccatt gtgagaaaag tcaggtgatt atggatgtgg aaaatcacct gtatgcgcgc
    27061 aaagacatta aacagctggg cattgcgccg atgaccagca tgttcagctg cggtactaat
    27121 gaacgtcgga tgtgcgacac cattcatccg caaatccatg actctgatcg tttgtccatg
    27181 tggcggggca atggcgagtg gatttgtcgt ccgctgaata atccgcaaaa attgcagttc
    27241 aatgcttaca ccgacaacaa cccgaaaggg tttggtttat tgcaactgga tcgtgatttc
    27301 tcccattatc aggacattat gggctggtat aacaaacgcc caagtctgtg ggtggagccg
    27361 cgtaacaagt ggggtaaggg caccatcggc ctgatggaaa tcccaacaac gggcgaaacg
    27421 ctggataaca ttgtctgctt ctggcagcca gaaaaagcgg taaaagcggg tgatgagttt
    27481 gcattccagt atcgtctgta ctggagtgcg caaccgcctg ttcattgccc attagcgcgc
    27541 gttatggcga cgcgtaccgg catgggcggt ttcccggaag gttgggcgcc aggtgaacac
    27601 tatcccgaaa aatgggcgcg acgttttgcc gtcgatttcg ttggtggtga tctgaaagct
    27661 gccgcgccaa aaggcattga gccggtgatt acgctttcca gtggggaagc gaaacaaatc
    27721 gaaattctct atattgaacc cattgatggt tatcgtattc agtttgactg gtatccgact
    27781 tcggactcca ctgatccggt cgatatgcgg atgtatctgc gttgtcaggg cgacgctatc
    27841 agtgaaacat ggctgtatca gtatttcccg ccagcgccgg ataaacgtca gtatgttgat
    27901 gaccgcgtga tgagttaatc gttttttctt cggcatcttc ttcgggaggt gccgtctggt
    27961 taaacacgat cccgctcgca tttttcccta agttaaatga gtaatctgat ggtgtgtatt
    28021 tcagatacac cttgtcagcc actaacaggg agtgcgtatg tttccagaat accgagattt
    28081 aatatcccgt ctgaaaaacg aaaatcctcg ctttatgtcc ttgttcgata aacacaataa
    28141 acttgatcat gaaattgcca gaaaggaagg ttccgacggt cgagggtaca atgcggaagt
    28201 ggtccgcatg aaaaaacaaa agctacagtt aaaagatgag atgctcaaaa tcctgcagca
    28261 ggtgagcgtc aaagaggtgt aaactttcct aagccgccag ccaggcggct tttttaacaa
    28321 ctgcatggat tgactggaga taagatgact gaaacgataa aagtaagtga atcacttgaa
    28381 ttacatgctg ttgcagaaag tcatgtcaca cctctttatc agttaatctg taaaaataga
    28441 acctggttac agcagtcgct aaactggccg cagtttgttc aaagtgaaga ggacacgcga
    28501 aaaacggtgc agggtaatgt gatgttgcat caacgcggct atgccaaaat gttcatgatt
    28561 ttcaaagaag atgaacttat cggcgttatc tcgtttaatc gtattgaacc actgaataaa
    28621 accgctgaaa tcggctactg gctggacgaa tctcatcagg ggcaggggat catttctcag
    28681 gcgctgcagg cattgattca tcattacgct cagtctggtg aacttagacg cttcgtgatc
    28741 aaatgtcggg tggacaatgc gcaaagcaac caggtcgctt tgcgcaatgg ttttatcctt
    28801 gaaggttgtc tgaaacaggc tgagttcctg aatgatgcct atgatgatgt gaacttatac
    28861 gcgcgtatta tcgattcaca ataaccctgc cagcggcgtt cgcgtaatgc gctcctcgcc
    28921 gttaatgact tttggaccac ggacatgaac cgtgtcgcca tcaaaggctt caacgacggc
    28981 gtggtcggtt aactccacat gtttttcaat tatcaccgca cctgtaatac ggctctcacc
    29041 ttgtattaca acgtgctcgt cgagcagaat cggtccgcca cgtaccacgg catttccgcc
    29101 aatcaagaca tgatgtttta acacacaatt accttccaca atggcatatt ccgccacctg
    29161 cgaactgtaa tgaatcgtcg gaatggcatc ttcttctatg ccagctttca cctgcgcgtg
    29221 accgtagact ttagcacaat cgcatagcca gacgttgttc tcttcattac cttcaacgct
    29281 ggcaaaatca aaaacttcgg cgcgatgttc aataaaagca taccggacga cggcatcgcc
    29341 ataaatttgt gcctggtgaa caatacgcga ggcgctaacc cttgcgcgat cataaatttg
    29401 tagcaggagt tgatggtcgg gcgttaagcc ttgtgcggcg acgatcatag aatgttgatc
    29461 aattaaggcg tgaccaaata ttctacattg cccgcaaact aatgagtcac gaatggtgac
    29521 actgtcactt atatatgcac cctgactgat ttcactatta tcgatccaga cgttatccgt
    29581 tgcgtaaacc tctccccaca gtacgctggt gccggtgatg cgcgtattac cggaaatcac
    29641 cgctccgccg aacgcaatag cattctggtc gtaaatccag caatttcctt cctgtgctaa
    29701 caccgtttcg caatcgatcc agccgccagc ggttcctgct atcacatcgt taaaatcgct
    29761 catggcgata atctgccgta ataacacact ttttttagtg ccatcatcct gataactaaa
    29821 ggctcgctgt tcttcactta gacgatattt gcgcatagcg ttttcccaca ggtgacttac
    29881 tataaccgta gcaaattctg cggctctggc tatgctcgag aaaattccat aaaatgcatt
    29941 tcaaatatac tttataaatt aaacaaaatg agtaagaaga tgcagagcga taaagtgctc
    30001 aatttgccgg caggctactt tggtattgtg ttggggacga tagggatggg atttgcctgg
    30061 cgctatgcca gccaggtttg gcaggtcagc cactggttag gggatgggct ggtgattctg
    30121 gcgatgatca tctggggatt attaactagc gcatttatta cccgactcat acgctttccg
    30181 catagcgtgc tggcggaagt gcgccatcca gtgctgagca gttttgtgag tttgtttccg
    30241 gcaacgacga tgctggtggc gattggtttt gttccgtggt ttcgcccact ggcggtgtgc
    30301 ctgttcagtt ttggcgtcgt ggttcagttg gcttatgccg cctggcaaac tgcgggatta
    30361 tggcgcggat ctcaccctga agaagctacc acgcctggac tgtatctgcc gacagttgcc
    30421 aacaacttta tcagcgcaat ggcctgtggt gcgttgggct acaccgacgc cggtctggtg
    30481 tttttaggcg caggcgtttt ctcatggcta agccttgaac ctgtgatctt gcagcgtctg
    30541 cgcagttcgg gagaattacc cacggcgctg cggacatcac tcggcattca gctcgctcct
    30601 gcgctggtgg cctgtagtgc ctggctgagc gtcaacggcg gcgagggtga cacgctggcg
    30661 aaaatgcttt tcggttatgg actgctgcaa ctgctgttta tgctacgtct gatgccatgg
    30721 tatctctccc agccatttaa tgcttcattc tggagtttct cgttcggcgt atctgcactg
    30781 gcaaccaccg gtttgcatct ggggagtggc agcgataatg gatttttcca tacgctggcg
    30841 gtgccgctgt ttatctttac caattttatt attgcaatac tgctcatccg tacttttgcg
    30901 cttctgatgc agggaaaatt gttaatcaga accgagcgcg ccgttttaat gaaagcagag
    30961 gacaaagaat gatcattcgt gacgaaaact attttactga taaatatgaa ttaactcgca
    31021 cacactctga agtgctggaa gcggtgaaag tggttaaacc gggtaaaacg ctggatctgg
    31081 gctgtggcaa tggtcgtaac agtctttacc tggcagccaa tggttatgat gttgacgcat
    31141 gggataaaaa tgccatgagt atcgccaacg tcgagcggat taaatccatt gaaaatctgg
    31201 agaatttaca cacccgggtc gttgatctga ataacctcac atttgatgga cagtatgatt
    31261 ttattctttc gactgtggtg ctgatgttcc ttgaggctaa aaccatccca gggctgattg
    31321 ccaatatgca acgttgcact aaacctggcg gttacaacct gattgtggcg gcgatggata
    31381 ccgctgatta tccatgtacc gtcggcttcc cgtttgcctt caaagagggg gaattacgtc
    31441 gatattacga aggctgggag atggtgaaat acaatgaaga cgtcggcgag ctgcaccgca
    31501 ccgacgccaa cggtaatcgt attaaactgc gtttcgccac gatgctggca cgtaaaaaat
    31561 gacccgctag gaacaaggcg cgtgaaaatt ccccacgctg agatggttta ctgttcttct
    31621 tttcggtaag catatttttt atcgaatgga tgtgaaatta atcacagtag tcgaaatttt
    31681 ttagcagctt aacttactga aatttaacta ctgatgattg acttagcccc tttttcggca
//
LOCUS       DAZFLD010000026        31496 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.026, whole genome shotgun sequence.
ACCESSION   DAZFLD010000026 DAZFLD010000000
VERSION     DAZFLD010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31496
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.026"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            86..190
                     /locus_tag="ACHKBL_001071"
     CDS             86..190
                     /locus_tag="ACHKBL_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5817977.1"
                     /translation="MENNSRTMPHIRRTTHIMKFAHRNSFDFHFFNAR"
     gene            448..2646
                     /gene="speF"
                     /locus_tag="ACHKBL_001072"
     CDS             448..2646
                     /gene="speF"
                     /locus_tag="ACHKBL_001072"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004586 - ornithine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase SpeF"
                     /protein_id="HFO5817978.1"
                     /translation="MSKLKIAVSDSCPDCFTTQRECIYINENSNIDVAAIVLSLNDVT
                     RGKLDEIDATGYGIPVFIATENQERVPAEYLPRISGVFENCESRREFYGRQLETAASH
                     YETQLRPPFFRALVDYVNQGNSAFDCPGHQGGEFFRRHPAGNQFVEYFGEALFRADLC
                     NADVAMGDLLIHEGAPCIAQQHAAKVFNADKTYFVLNGTSSSNKVVLNALLTPGDLVL
                     FDRNNHKSNHHGALLQAGATPVYLETARNPYGFIGGIDAHCFEESYLRELIAEFAPQR
                     AKEARPFRLAVIQLGTYDGTIYNARQVVDKIGHLCDYILFDSAWVGYEQFIPMMADCS
                     PLLLDLNENDPGILVTQSVHKQQAGFSQTSQIHKKDSHIKGQQRYVPHKRMNNAFMMH
                     ASTSPFYPLFAALDINAKMHEGVSGRNMWMDCVVNGINARKLILDNCQHIRPFVPELV
                     DGKPWQSYETAQIAVDLRFFQFVPGEHWHSFEGYAENQYFVDPCKLLLTTPGIDARNG
                     EYEAFGVPATILANFLRENGVVPEKCDLNSILFLLTPAEDMAKLQQLVALLVRFEKLL
                     ESDAPLSEVLPSIYKQHEERYTGYTLRQLCQEMHDLYARHNVKQLQKEMFRKEHFPRV
                     SMNPQEANYAYLRGEVELVRLPDAEGRIAAEGALPYPPGVLCVVPGEIWGGAVLRYFS
                     ALEEGINLLPGFAPELQGVYIEEHDGRKQVWCYVIKPRDAQSTLLKGEKL"
     gene            2643..3962
                     /gene="potE"
                     /locus_tag="ACHKBL_001073"
     CDS             2643..3962
                     /gene="potE"
                     /locus_tag="ACHKBL_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015496 - putrescine:ornithine antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:0015847 - putrescine transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine-ornithine antiporter"
                     /protein_id="HFO5817979.1"
                     /translation="MSQAKSNKMGVVQLTILTMVNMMGSGIIMLPTKLAEVGTISIIS
                     WLVTAVGSMALAWAFAKCGMFSRKSGGMGGYAEYAFGKSGNFMANYTYGVSLLIANVA
                     IAISAVGYGTELLGASLSPVQIGLATIGVLWICTVANFGGARITGQISSITVWGVIIP
                     VVGLCIIGWFWFSPTLYANSWNPHHVPFFSAVGSSIAMTLWAFLGLESACANAEVVEN
                     PERNVPIAVLGGTLGAAVIYIVSTNVIAGIVPNMELANSTAPFGLAFAQMFTPEVGKV
                     IMALMVMSCCGSLLGWQFTIAQVFKSSADEGYFPKIFSRVSKVDAPVQGMLTIVIIQS
                     GLSLMTISPSLNSQFNVLVNLAVVTNIIPYILSMAALVIIQKVANVPPSKAKVANFVA
                     FVGAMYSFYALYSSGEEAMLYGSIVTFLGWTLYGLVSPRFELKNKHG"
     gene            complement(4019..5659)
                     /gene="pgm"
                     /locus_tag="ACHKBL_001074"
     CDS             complement(4019..5659)
                     /gene="pgm"
                     /locus_tag="ACHKBL_001074"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004614 - phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase
                     (alpha-D-glucose-1,6-bisphosphate-dependent)"
                     /protein_id="HFO5817980.1"
                     /translation="MAIHNRAGQPAQQSDLINVAQLTAQYYVLKPEAGNAEHAVKFGT
                     SGHRGSAARHSFNEPHILAIAQAIAEERAKNGITGPCYVGKDTHALSEPAFISVLEVL
                     AANGVDVIVQENNGFTPTPAISNAILVHNKKGGPLADGIVITPSHNPPEDGGIKYNPP
                     NGGPADTNVTKVVEDRANALLADGLKGVKRISLDEAMASGHVKEQDLVQPFVEGLADI
                     VDMAAIQKAGLTLGVDPLGGSGIEYWKRIGEYYNLNLTIVNDQVDQTFRFMHLDKDGA
                     IRMDCSSECAMAGLLALRDKFDLAFANDPDYDRHGIVTPAGLMNPNHYLAVAINYLFQ
                     HRPQWGKDVAVGKTLVSSAMIDRVVNDLGRKLVEVPVGFKWFVDGLFDGSFGFGGEES
                     AGASFLRFDGTPWSTDKDGIIMCLLAAEITAVTGKNPQEHYNELAKRFGAPSYNRLQA
                     AATSAQKAALSKLSPEMVSASTLAGDPITARLTAAPGNGASIGGLKVMTDNGWFAARP
                     SGTEDAYKIYCESFLGEEHRKQIEKEAVEIVSEVLKNA"
     gene            complement(5685..6230)
                     /gene="seqA"
                     /locus_tag="ACHKBL_001075"
     CDS             complement(5685..6230)
                     /gene="seqA"
                     /locus_tag="ACHKBL_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032297 - negative regulation of
                     DNA-templated DNA replication initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HFO5817981.1"
                     /translation="MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSAASQPAAP
                     VTKEVRVASPAIVEAKPVKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLD
                     AQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
                     EHIMQSMQFPAELIEKVCGTI"
     gene            6415..7179
                     /gene="ybfF"
                     /locus_tag="ACHKBL_001076"
     CDS             6415..7179
                     /gene="ybfF"
                     /locus_tag="ACHKBL_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFO5817982.1"
                     /translation="MKLNIRAQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHN
                     IIQVDMRNHGLSPRDPVMNYPAMAQDLVDTLDAQQIDKATFIGHSMGGKAVMALTALA
                     PDRIDKLVAIDIAPVDYHVRRHDEIFAAINAVSESDAQTRQQAAAIMRQHLNEEGVIQ
                     FLLKSFVDGEWRFNVPVLWDQYPHIVGWEKIPAWDHPTLFIPGGNSPYVSEQYRDDLL
                     AQFPQARAHVIAGAGHWVHAEKPDAVLRAIRRYLND"
     gene            7319..7612
                     /gene="ybfE"
                     /locus_tag="ACHKBL_001077"
     CDS             7319..7612
                     /gene="ybfE"
                     /locus_tag="ACHKBL_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HFO5817983.1"
                     /translation="MAKEQTDRTTLDLFAHERRPGRPKTNPLSRDEQLRINKRNQLKR
                     DKVRGLKRVELKLNAEAVEALNELAESRNMSRSELIEEMLMQQLAALRSQGIV"
     gene            7752..8282
                     /gene="fldA"
                     /locus_tag="ACHKBL_001078"
     CDS             7752..8282
                     /gene="fldA"
                     /locus_tag="ACHKBL_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HFO5817984.1"
                     /translation="MAITGIFFGSDTGNTENIAKMIQKQLGKDVADVHDIAKSSKEDL
                     EAYDILLLGIPTWYYGEAQCDWDDFFPTLEEIDFNGKLVALFGCGDQEDYAEYFCDAL
                     GTIRDIIEPRGATIVGHWPTAGYHFEASKGLADDDHFVGLAIDEDRQPELTAERVEKW
                     VKQISEELHLDEILNA"
     gene            8492..8578
                     /gene="uof"
                     /locus_tag="ACHKBL_001079"
     CDS             8492..8578
                     /gene="uof"
                     /locus_tag="ACHKBL_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fur leader peptide"
                     /protein_id="HFO5817985.1"
                     /translation="MIRIISRANSVTSPNEVNRLVTGQIPHD"
     gene            8571..9017
                     /gene="fur"
                     /locus_tag="ACHKBL_001080"
     CDS             8571..9017
                     /gene="fur"
                     /locus_tag="ACHKBL_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HFO5817986.1"
                     /translation="MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLI
                     DMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEF
                     SDDSIEARQREIAAKHGIRLTNHSLYLYGHCAEGDCREDEHAHEGK"
     gene            complement(9101..9427)
                     /gene="chiQ"
                     /locus_tag="ACHKBL_001081"
     CDS             complement(9101..9427)
                     /gene="chiQ"
                     /locus_tag="ACHKBL_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChiQ/YbfN family lipoprotein"
                     /protein_id="HFO5817987.1"
                     /translation="MKKLILIAMMASGLVACAQSTAPQEDSRLKEAYSACINTAQGSP
                     EKIEACQSVLNVLKKEKQHQQFAEQESVRVLDYQQCIQATQTGNDQAVKADCDKVWQE
                     IRSNNK"
     gene            complement(9477..10883)
                     /gene="chiP"
                     /locus_tag="ACHKBL_001082"
     CDS             complement(9477..10883)
                     /gene="chiP"
                     /locus_tag="ACHKBL_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitoporin"
                     /protein_id="HFO5817988.1"
                     /translation="MRTFSGKRSTLALAIAGVTAMSGFMAMPEARAEGFIDDSTLTGG
                     IYYWQRERDRKDVTDGDKYKTNLSHSTWNANLDFQSGYAADMFGLDIAAFTAIEMAEN
                     GDSSHPNEIAFSKSNKAYDEDWSGDKSGISLYKAAAKFKYGPVWARAGYIQPTGQTLL
                     APHWSFMPGTYQGAEAGANFDYGDAGALSFSYMWTNEYKAPWHLEMDEFYQNDKTTKV
                     DYLHSIGAKYDFKNNFVLEAAFGQAEGYIDQYFAKASYKFDIAGSPLTTSYQFYGTRD
                     KVDDRSVNDIYDGTAWLQALTFGYRAADVVDLRLEGSWVKADGQQGYFLQRMTPTYAS
                     SNGRLDIWWDNRSDFNANGEKAVFFGAMYDLKNWNLPGFAIGASYVYAWDAKPATWQS
                     NPDAYYDKNRTIEESAYSLDAVYTIQDGRAKGTMFKLHFTEYDNHSDIPSWGGGYGNI
                     FQDERDVKFMVIAPFTIF"
     gene            complement(11332..12996)
                     /gene="glnS"
                     /locus_tag="ACHKBL_001083"
     CDS             complement(11332..12996)
                     /gene="glnS"
                     /locus_tag="ACHKBL_001083"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HFO5817989.1"
                     /translation="MSEAEARPTNFIRQIIDEDLASGKHTTVHTRFPPEPNGYLHIGH
                     AKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVESIKNDVEWLGFHWSGNVRYSSD
                     YFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEK
                     MRTGSFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCI
                     SDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNL
                     LVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTKQDNTIEMASLESCIRE
                     DLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEMGSRQVPFSCEIWIDRAD
                     FREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPADG
                     RKVKGVIHWVSAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPSL
                     KDAVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDTWAKVGE"
     gene            complement(13199..15145)
                     /gene="nagE"
                     /locus_tag="ACHKBL_001084"
     CDS             complement(13199..15145)
                     /gene="nagE"
                     /locus_tag="ACHKBL_001084"
                     /EC_number="2.7.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015572 - N-acetylglucosamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetyl glucosamine transporter subunit
                     IIABC"
                     /protein_id="HFO5817990.1"
                     /translation="MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQA
                     GGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGYFVLTKAMVTINPEINMGVLAGI
                     ITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIH
                     AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINR
                     FYAGDGTAGMFMSGFFPIMMFGLPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGV
                     TEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAGAIDYALMYNLPAAS
                     QNVWMLLVMGVVFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLNQL
                     ATNYIAAVGGTDNLKAIDACITRLRLTVVDSARVNDVMCKRLGASGVVKLNKQTIQVI
                     VGAKAESIGDAMKKVVARGPVAAASAETAPATAAPVAKPQAVPNAVSIAELVSPITGD
                     VVALDQVPDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLETEKGAEI
                     VVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILEMDLDYLNANARSMISPVVCSNIDD
                     FSGLIIKAQGHVVAGQTPLYEIKK"
     gene            15478..16278
                     /gene="nagB"
                     /locus_tag="ACHKBL_001085"
     CDS             15478..16278
                     /gene="nagB"
                     /locus_tag="ACHKBL_001085"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFO5817991.1"
                     /translation="MRLIPLTTAEQVGKWAARHIVNRINAFKPTADRPFVLGLPTGGT
                     PMTTYKALVEMHKAGQVSFKHVVTFNMDEYVGLPKEHPESYYSFMHRNFFDHVDIPAE
                     NINLLNGNAPDIDAECRQYEEKIRSYGKIHLFMGGVGNDGHIAFNEPASSLASRTRIK
                     TLTHDTRVANSRFFDNDVNQVPKYALTVGVGTLLDAEEVMILVLGSQKALALQAAVEG
                     CVNHMWTISCLQLHPKAIMVCDEPSTMELKVKTLRYFNELEAENIKGL"
     gene            16338..17486
                     /gene="nagA"
                     /locus_tag="ACHKBL_001086"
     CDS             16338..17486
                     /gene="nagA"
                     /locus_tag="ACHKBL_001086"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFO5817992.1"
                     /translation="MYALTQGRIFTGHEFLDDHAVVIADGLIKSVCPVAELPPEIEQR
                     SLNGAILSPGFIDVQLNGCGGVQFNDTAEAVSVETLEIMQKANEKSGCTNYLPTLITT
                     SDELMKQGVRVMREYLAKHPNQALGLHLEGPWLNLVKKGTHNPNFVRKPDAALVDFLC
                     ENADVITKVTLAPEMVPAEVISKLANAGIVVSAGHSNATLKEAKAGFRAGITFATHLY
                     NAMPYITGREPGLAGAILDEADIYCGIIADGLHVDYANIRNAKRLKGDKLCLVTDATA
                     PAGANIEQFIFAGKTIYYRNGLCVDENGTLSGSSLTMIEGVRNLVEHCGIALDEVLRM
                     ATLYPARAIGVEKRLGTLAAGKVANLTAFTPDFKITRTIVNGNEVVTQ"
     gene            17495..18715
                     /gene="nagC"
                     /locus_tag="ACHKBL_001087"
                     /gene_synonym="nagR"
     CDS             17495..18715
                     /gene="nagC"
                     /locus_tag="ACHKBL_001087"
                     /gene_synonym="nagR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NagC"
                     /protein_id="HFO5817993.1"
                     /translation="MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQL
                     APASVTKITRQLIERGLIKEVDQQASTGGRRAISIVTETRNFHAIGVRLGRHDATITL
                     FDLSSKVLAEEHYPLPERTQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDP
                     DSGKIHYMPHIQVENWGLVEALEERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILV
                     RVHRGTGAGIISNGRIFIGRNGNVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQ
                     RVLNLLKQGYQSRVPLDDCTIKTICKAANKGDSLASEVIEYVGRHLGKTIAIAINLFN
                     PQKIVIAGEITEADKVLLPAIESCINTQALKAFRTNLPVVRSELDHRSAIGAFALVKR
                     AMLNGILLQHLLEN"
     gene            18763..19515
                     /gene="nagD"
                     /locus_tag="ACHKBL_001088"
     CDS             18763..19515
                     /gene="nagD"
                     /locus_tag="ACHKBL_001088"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide monophosphatase NagD"
                     /protein_id="HFO5817994.1"
                     /translation="MTIKNVICDIDGVLMHDNVAVPGAAEFLHGIMDKGLPLVLLTNY
                     PSQTGQDLANRFATAGVDVPDSVFYTSAMATADFLRRQEGKKAYVVGEGALIHELYKA
                     GFTITDVNPDFVIVGETRSYNWDMMHKAAYFVANGARFIATNPDTHGRGFYPACGALC
                     AGIEKISGRKPFYVGKPSPWIIRAALNKMQAHSEETVIVGDNLRTDILAGFQAGLETI
                     LVLSGVSSLDDIDSMPFRPSWIYPSVAEIDVI"
     gene            19771..21435
                     /gene="asnB"
                     /locus_tag="ACHKBL_001089"
     CDS             19771..21435
                     /gene="asnB"
                     /locus_tag="ACHKBL_001089"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HFO5817995.1"
                     /translation="MCSIFGVFDIKTDAVELRKKALELSRLMRHRGPDWSGIYASDNA
                     ILAHERLSIVDVNAGAQPLYNQQKTHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEV
                     ILALYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVAS
                     EMKALVPVCRTIKEFPAGSYLWSQDGEIRSYYHRDWFDYDAVKDNVTDKNELRQALED
                     SVKSHLMSDVPYGVLLSGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLP
                     GSPDLKAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMS
                     RKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPNAKELHEETVRKLLALHMYDCARANKA
                     MSAWGVEARVPFLDKKFLDVAMRINPQDKMCGNGKMEKHILRECFEAYLPASVAWRQK
                     EQFSDGVGYSWIDTLKEVAAQQVSDQQLETARFRFPYNTPTSKEAYLYREIFEELFPL
                     PSAAECVPGGPSVACSSAKAIEWDEAFKKMDDPSGRAVGVHQSAYK"
     gene            21816..21892
                     /locus_tag="ACHKBL_001090"
     tRNA            21816..21892
                     /locus_tag="ACHKBL_001090"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21850..21852,aa:Met,seq:cat)
     gene            21901..21985
                     /locus_tag="ACHKBL_001091"
     tRNA            21901..21985
                     /locus_tag="ACHKBL_001091"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21935..21937,aa:Leu,seq:tag)
     gene            22009..22083
                     /locus_tag="ACHKBL_001092"
     tRNA            22009..22083
                     /locus_tag="ACHKBL_001092"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22041..22043,aa:Gln,seq:ttg)
     gene            22099..22175
                     /locus_tag="ACHKBL_001093"
     tRNA            22099..22175
                     /locus_tag="ACHKBL_001093"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22133..22135,aa:Met,seq:cat)
     gene            22224..22298
                     /locus_tag="ACHKBL_001094"
     tRNA            22224..22298
                     /locus_tag="ACHKBL_001094"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22256..22258,aa:Gln,seq:ctg)
     gene            complement(22452..23627)
                     /gene="ubiF"
                     /locus_tag="ACHKBL_001095"
     CDS             complement(22452..23627)
                     /gene="ubiF"
                     /locus_tag="ACHKBL_001095"
                     /EC_number="1.14.99.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-demethoxyubiquinol 3-hydroxylase"
                     /protein_id="HFO5817996.1"
                     /translation="MTNQPTEIAIVGGGMVGGALALGLAQHGFSVMVIEHAQPAPFVA
                     DSQPDVRISAISAASVSLLKGLGVWDAVQAMRCHPYRRLETWEWETAHVVFDAAELKL
                     PLLGYMVENTVLQQALWQALEAHPKVTLRVPTSLIALHRHNDLQELELKGGETILAKL
                     VIGADGANSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPL
                     FDNWASLVWYDSPARIRQLQNMNMAQLQAEIAKHFPSRLGYVTPLAAGAFPLIRRHAL
                     QYVQPGLALVGDAAHTIHPLAGQGVNLGYRDVDALIDVLVNARSYGEAWASYPVLKRY
                     QMRRMADNFMMQSGMDLFYAGFSNNLPPLRFVRNLGLMAAERAGVLKRKALKYALGL"
     gene            23773..25197
                     /gene="miaB"
                     /locus_tag="ACHKBL_001096"
     CDS             23773..25197
                     /gene="miaB"
                     /locus_tag="ACHKBL_001096"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HFO5817997.1"
                     /translation="MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVL
                     LLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDII
                     FGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
                     CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGSF
                     ADLLRLVAAIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNL
                     MGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPGETTEDFEKTMKLIADVNFDMSY
                     SFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGT
                     SRKSIMELSGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRV
                     AETPESVIARTRKENDLGVGYYQP"
     gene            25350..26390
                     /gene="ybeZ"
                     /locus_tag="ACHKBL_001097"
     CDS             25350..26390
                     /gene="ybeZ"
                     /locus_tag="ACHKBL_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HFO5817998.1"
                     /translation="MNIDTREITLEPADNARLLSLCGPFDDNIKQLERRLGIEINRRD
                     NHFKLTGRPICVTAAADILRSLYVDTAPMRGQIQDIEPEQIHLAIKEARVLEQSAESV
                     PEYGKAVNIKTKRGVIKPRTPNQAQYIANILDHDITFGVGPAGTGKTYLAVAAAVDAL
                     ERQEIRRILLTRPAVEAGEKLGFLPGDLSQKVDPYLRPLYDALFEMLGFEKVEKLIER
                     NVIEVAPLAYMRGRTLNDAFIILDESQNTTIEQMKMFLTRIGFNSKAVITGDVTQIDL
                     PRNTKSGLRHAIEVLADVEEISFNFFHSEDVVRHPVVARIVNAYEAWEEAEQKRKAAL
                     AAERKREEQEQK"
     gene            26387..26854
                     /gene="ybeY"
                     /locus_tag="ACHKBL_001098"
     CDS             26387..26854
                     /gene="ybeY"
                     /locus_tag="ACHKBL_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014169133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HFO5817999.1"
                     /translation="MSQVILDLQLACEDNSGLPEESQFQTWLNAVIPQFQEESEVTIR
                     VVDTAESHSLNLTYRGKDKPTNVLSFPFEVPPGMEMSLLGDLVICRQVVEKEAQEQGK
                     PLEAHWAHMVVHGSLHLLGYDHIEDDEAEEMEAIETEIMLALGYEDPYIAEKE"
     gene            26944..27822
                     /gene="corC"
                     /locus_tag="ACHKBL_001099"
     CDS             26944..27822
                     /gene="corC"
                     /locus_tag="ACHKBL_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308723.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HFO5818000.1"
                     /translation="MSDDNSHSSDTISNKKGFFSLLLSQLFHGEPKNRDELLALIRDS
                     GQNDLIDEDTRDMLEGVMDIADQRVRDIMIPRSQMITLKRNQTLDECLDVIIESAHSR
                     FPVISEDKDHIEGILMAKDLLPFMRSDAEAFSMDKVLRQAVVVPESKRVDRMLKEFRS
                     QRYHMAIVIDEFGGVSGLVTIEDILELIVGEIEDEYDEEDDIDFRQLSRHTWTVRALA
                     SIEDFNEAFGTHFSDEEVDTIGGLVMQAFGHLPARGETIDIDGYQFKVAMADSRRIIQ
                     VHVKIPDDSPQPKLDE"
     gene            27847..29385
                     /gene="lnt"
                     /locus_tag="ACHKBL_001100"
     CDS             27847..29385
                     /gene="lnt"
                     /locus_tag="ACHKBL_001100"
                     /EC_number="2.3.1.269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001566593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HFO5818001.1"
                     /translation="MAFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIISLMGL
                     QALTFNRRPLQSAAIGFCWGFGLFGSGINWVYVSIATFGGMPGPVNIFLVVLLAAYLS
                     LYTGLFAGVLSRLWPKTTWLRVAIAAPALWQVTEFLRGWVLTGFPWLQFGYSQIDGPL
                     KGLAPIMGVEAINFLLMMVSGLLALALVKRNWRPLVVAVVLFALPFPLRYIQWFTPQP
                     EKTIQVSMVQGDIPQSLKWDGDQLLNTLKIYYNATAPLMGKSSLIIWPESAITDLEIN
                     QQPFLKALDGELRDKGSSLVTGIVDARLNKQNRYDTYNTIITLGKGAPYSYESADRYN
                     KNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSLNGIQLTAAICYEIIL
                     GEQVRDNFRPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITA
                     VIGPQGEIQAMIPQFTREVLTTNVTPTTGLTPYARTGNWPLWVLTALFGFAAVLMSLR
                     ARKH"
     gene            complement(29463..31340)
                     /locus_tag="ACHKBL_001101"
     CDS             complement(29463..31340)
                     /locus_tag="ACHKBL_001101"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HFO5818002.1"
                     /translation="MSASSVKPLNVQLPAITLILFALCVGIFCYLAQWMSYEEVDQSA
                     LIHLGANVASLTLSGESWRLLSSVFLHSSVSHLLMNMFALLVVGGAVERILGKWRLLI
                     IWLFSGIFGGLISACYALRESEQIVISVGASGAIMGIAGAAIATQLSSGAGTHHKNQR
                     RVFSLLGMVALTLLYGARQAGIDNACHIGGLIAGGALGWLSARLVGQNRLVTEGGIIV
                     AVTLLLTGTIWFVQQQIDESVLQVRQSLREAFYPQEIEQERRQKKQQLVEERNALRET
                     LSAPVSREQASGDLLAEIADIHDMAISRDGNTLYAAIENTNSIVVFDLGQKKILHTFT
                     APIAKEKSVKHCGGCKDQGVRSLALSLDEKLIYATSFEANAVSVINVATGEIIQSITT
                     GAHPDSLILSRDGTKAWVMNRTSNSVSAIDLVTYQHVTDIPLEKYDGTGTSGKPGAWV
                     MALSPDEKTLLIPGMVRGDIVRINTITHQKEDFPTGDARGIVSAMGFRPKNGEIIFAD
                     SQGISRISVGDQQASIMTQWCSRSVYSVEGISPDGQYLALVSYGLQGYVILLNINAGQ
                     IIGVYPASYVNHLRFSADGRKIFVMAKNRLIQMDRTRSLDPQAIIRHPQYGDVACIPE
                     P"
BASE COUNT         7731 a         8151 c         7917 g         7697 t
ORIGIN      
        1 ttttttattg atcaccttca cagttcaacc gtatttcctg gatagaatac cttcaccttc
       61 attcacatca ggaaaggtaa attaaatgga aaataacagc cgcactatgc cccatataag
      121 gcggacaact catattatga agtttgctca tcgcaatagc ttcgactttc acttcttcaa
      181 tgcccgttag tctaccgact aagggcactt cagcgtacag gtcttcctga ctctctgtat
      241 tacaggcata tgcctgtatt ccgcagtgct cattctctgt taacccttgt attatcagcc
      301 accggactga taagggtttc tgcattctga aaaaagcaaa cttctgatta atttatcagc
      361 ggggtaactt tgcttttttt ccggcacgat cgatttctca ttcgagaaat tgaggacctg
      421 ctattaccca aaataaagag atggaaaatg tcaaaattaa aaattgcggt tagtgattct
      481 tgcccggact gttttaccac gcagcgagaa tgtatctata ttaatgaaaa cagtaatatc
      541 gatgtggcgg caatagtttt atcgctcaac gatgttacac gcggaaaact cgatgaaatc
      601 gatgccacgg gttatggcat cccggtattt attgctactg aaaatcaaga acgtgtaccc
      661 gcagagtatt tgccccgtat ttcgggtgtc tttgagaatt gcgaatcgcg acgagaattt
      721 tatggtcgtc agttagaaac cgcagccagc cattatgaaa cgcaactgcg cccgcccttc
      781 ttccgcgcac tggtcgatta tgtcaatcag ggtaacagcg cgtttgattg ccccggtcat
      841 cagggcggcg aatttttccg tcgccatccc gcggggaatc agtttgtgga atactttggt
      901 gaggcgctgt tccgtgctga cttgtgcaac gccgacgtag cgatgggcga tctgctgatt
      961 cacgaaggcg cgccatgcat tgcacagcaa catgcggcaa aagtgtttaa tgccgataaa
     1021 acatacttcg ttttaaatgg cacttcatct tctaacaaag tggttttaaa cgccctgctg
     1081 acaccgggtg atctggtgct gtttgaccgc aataaccaca aatctaacca ccacggagcg
     1141 ttgctacagg ctggcgcgac accggtttat ctggaaaccg cacgtaaccc gtatggtttt
     1201 atcggtggta ttgacgctca ctgttttgaa gaaagttacc tgcgtgaact gatcgcggaa
     1261 ttcgcaccgc agcgggcaaa agaggctcgt cctttccgcc tcgccgttat tcagctaggc
     1321 acttacgacg gtacgattta taacgcccgt caggtggtgg ataaaattgg tcatctgtgt
     1381 gactacatcc tctttgactc cgcatgggtc ggctatgagc agtttatccc gatgatggcg
     1441 gactgttcac cactgttgct ggatcttaat gagaacgatc cgggtattct ggttacgcaa
     1501 tctgtgcata aacagcaggc tggtttttcc cagacttcac aaatccataa aaaagacagc
     1561 catatcaaag ggcaacagcg ttatgtaccg cacaaacgca tgaacaacgc ctttatgatg
     1621 catgcctcca ccagcccgtt ctatccgctg tttgccgcac tggatatcaa cgccaaaatg
     1681 catgaaggtg tcagcggtcg taatatgtgg atggattgtg tggtaaatgg cattaatgcc
     1741 cgcaaactaa tcctcgataa ctgtcagcat attcgtccat tcgtacctga actggtggat
     1801 ggtaaaccct ggcagtcgta tgaaacggcg caaattgcgg ttgatctgcg cttcttccag
     1861 tttgtaccag gtgagcactg gcattctttt gaaggctatg cagagaatca atactttgtc
     1921 gatccgtgca aactgttgct gacaacccca ggtattgatg cacgtaacgg cgaatatgaa
     1981 gcgttcggtg tacccgcgac gattcttgct aacttcctgc gcgaaaatgg cgtagtgccg
     2041 gaaaaatgcg atcttaactc catcctcttt ctgctgactc cggcagaaga tatggccaaa
     2101 cttcagcaac ttgttgctct gctggtacgc ttcgaaaaac tgcttgagtc cgacgcgcca
     2161 ttatcagaag tactgccttc catctacaaa cagcatgaag agcgctacac cggttatacc
     2221 ctgcgtcagt tgtgtcagga aatgcatgat ttgtatgccc gtcacaacgt gaaacaactg
     2281 caaaaagaga tgttccgtaa ggaacacttc ccacgcgtaa gcatgaatcc acaagaagcc
     2341 aactacgcct atttacgcgg tgaagtggag ctggttcgtc tgccggatgc agaaggccgt
     2401 atcgctgccg aaggtgcgct tccttatcct ccgggtgtgc tgtgtgttgt tccgggtgaa
     2461 atctggggtg gtgctgttct gcgttacttc agcgctctgg aagaagggat caacctgctg
     2521 ccaggttttg caccagagct gcagggtgtc tatatcgaag aacatgatgg tcgtaagcaa
     2581 gtttggtgct atgtcatcaa gcctcgtgat gcgcaaagca ccctgttgaa aggggaaaaa
     2641 ttatgagtca ggctaaatcg aataagatgg gcgtcgttca gttaaccata ctgacgatgg
     2701 tcaacatgat gggctccggt atcatcatgc tgccgacaaa gcttgccgaa gttgggacaa
     2761 tctcaattat ctcctggctg gtgacagccg taggctcaat ggcactggca tgggcattcg
     2821 caaaatgcgg tatgttcagc cgtaaatcag gcggtatggg cggttatgcg gagtatgctt
     2881 tcggtaaatc cgggaacttt atggcgaact acacctacgg tgtctcgctg ctgattgcta
     2941 acgtcgcgat tgctatttcg gcggttggtt acggcaccga actgctcggc gcgagtttgt
     3001 cgccagtgca gattggtctt gcgaccatcg gagtgctgtg gatttgtacc gtggctaact
     3061 ttggtggtgc gcgcattacc gggcaaatca gtagcattac tgtgtggggg gtcattattc
     3121 cggtcgtcgg tctgtgcatt atcggctggt tctggtttag cccgacgcta tacgctaact
     3181 catggaaccc gcaccatgtg ccgttcttta gcgctgtagg ttcttccatc gccatgacgt
     3241 tgtgggcttt cctcggtctg gagtcagcct gtgccaacgc cgaagtagtt gaaaacccgg
     3301 aacgtaacgt gccgattgca gttcttggcg gtacgttagg tgcggcggtg atttatatcg
     3361 tctccaccaa cgtgattgcc gggattgtgc caaatatgga gctggcaaat tcaacggcac
     3421 catttggtct ggccttcgcg cagatgttca cgccggaagt gggtaaagtc attatggcgc
     3481 tgatggtgat gtcctgctgc ggttcgctgc ttggctggca gttcaccatt gcccaggtgt
     3541 ttaaatcttc tgccgatgaa gggtacttcc cgaaaatttt ctcccgtgtg agcaaagtcg
     3601 atgcaccagt acagggaatg ctgaccattg tcatcatcca gagcggatta tcactgatga
     3661 ccattagccc gtcgctgaac agtcagttca acgtgctggt taacctggcc gtggtgacca
     3721 atatcattcc gtatattctg tcaatggcgg cattagtcat tattcagaag gtcgctaatg
     3781 tgcccccctc aaaagcgaaa gttgcaaact ttgttgcttt tgttggcgcg atgtacagct
     3841 tttatgcgct gtactcatcc ggggaagaag ccatgctata cggttccatc gtgaccttcc
     3901 tcggttggac actgtatggt ctggtctcac cacgctttga actgaaaaat aaacacggtt
     3961 aattaagcaa agcaaaaaag ggcgatcttc cgaccgccct ttttcttatt tgatatgttt
     4021 acgcgttttt cagaacttcg ctaacaatct caaccgcttc tttctcaatc tgcttgcgat
     4081 gttcttcacc aaggaagctt tcgcagtaga tcttatatgc gtcttccgtg cctgacggac
     4141 gcgcggcgaa ccagccgttg tcagtcatca ctttcagacc gccgatagaa gcgccattac
     4201 ccggtgccgc cgtcagacgc gcggtgatcg ggtcacctgc cagggtgctg gcgctcacca
     4261 tttccggaga cagcttagac aacgccgctt tttgtgcgga agtcgctgct gcctgcaaac
     4321 ggttatagct cggcgcacca aagcgttttg ccagttcgtt atagtgttcc tgcgggttct
     4381 taccggtgac agcggtgatt tccgccgcca gcagacacat aatgataccg tctttatcgg
     4441 tggaccacgg cgtgccgtcg aaacgcagga aggaagcgcc tgcactctct tcgccgccaa
     4501 agccgaagct gccatcgaac aaaccatcaa caaaccattt gaaacctacc ggtacttcta
     4561 ccagcttacg gcccaaatca ttgaccacac ggtcgatcat cgcagatgaa accagcgttt
     4621 taccgacagc aacatctttg ccccactgcg gacgatgctg gaacaggtag ttgatcgcca
     4681 ccgccaggta gtggttcgga ttcatcaaac ctgccggagt gacgataccg tgacggtcat
     4741 aatccgggtc gttagcaaac gccagatcga acttatcacg cagtgccagc aggcccgcca
     4801 tcgcacactc ggaggagcag tccatacgga tcgcaccgtc tttatcgagg tgcataaagc
     4861 ggaaggtttg atcgacctga tcgttaacga tggtcaggtt gaggttgtaa tactcgccaa
     4921 tgcgcttcca gtattcgata ccggaaccac ccagcggatc aacgcccagc gtcaggcccg
     4981 ctttctgaat cgccgccata tcaacgatat cggccagccc ttccacaaac ggctgcacca
     5041 gatcctgctc tttcacatga ccggatgcca tcgcttcgtc gagggagata cgcttcacgc
     5101 ctttcaggcc atcagccagc agtgcgttgg ctctgtcttc caccacttta gtgacgttgg
     5161 tatcagccgg gccaccattt ggcggattgt acttgatacc accatcttcc ggcgggttat
     5221 gggacggtgt aatcacgata ccgtctgcca gtgggccacc ttttttattg tgaaccagaa
     5281 tggcattgga aatggcaggt gtcggagtga agccattgtt ttcctgcaca atgacatcaa
     5341 cgccgttcgc tgccagcact tccagtacgg aaatgaacgc aggctcggac agggcgtgag
     5401 tatctttacc cacatagcaa gggccagtga tgccgttttt cgcacgttct tcagcaattg
     5461 cctgagcgat cgccagaatg tgcggctcgt taaagctgtg gcgcgctgca ctgccacggt
     5521 gaccggaagt accgaatttc accgcgtgct ccgcattccc tgcttctggt ttcagtacat
     5581 aatattgcgc cgtcagttgg gcgacgttaa tcaaatcact ctgttgtgca ggttggcctg
     5641 cacgattgtg gattgccatt gctttgtcct ttgtctgcaa cgttttagat agttccgcaa
     5701 accttctcaa tcaattccgc cgggaattgc atcgactgca tgatgtgttc gatcatgctg
     5761 catttacggc cggtgttggt gttggtgatc acccaatacg gcgtaccagg cacatgtttc
     5821 ggcttggtct gattaccatt tttcagcagc gtttgttcat ctgccgcaaa gtaaacgcgt
     5881 gtacgaccgt gcaacgattc cgttgcttcg gcaaacgcct gggcgtcaag agaatatagt
     5941 gtagacaaca gcagcataaa gcgattgacc gctcgctttt gctctgcgta ttcgtccgaa
     6001 agcagaagtt cacgcattgc gcgaaccttg tctttaatcg ttttgaccgg cttcgcttcg
     6061 acgatagcag gtgacgcaac gcgaacctct ttcgtcaccg gagcagcagg ctgtgatgcg
     6121 gcggaaaatt tcaacatacg ccgtaaaatg tcggatgcgc tctcgccgat atgcttagtg
     6181 tggctggcaa tatagctgta gagttcatca tcaacttcaa tcgttttcat cttaatccag
     6241 tgcagtgtct tagctgaata caagtcgcca ggagtatatg aataaatccc ggcagcggat
     6301 agcgtcaagc ctggcttgca gaaaaactcg gaataaacct tatttgttgc agccctgtgg
     6361 cacaatggtc aacataatac cctaacccga caacgcgaaa aaaagaactt tgccatgaaa
     6421 ttgaatatcc gcgcgcaaac tgcacaaaac cagcacaata attctcccat cgttcttgtc
     6481 catggtctgt ttggcagcct cgacaacctt ggcgtactgg ctcgcgatct ggtaaacgat
     6541 cacaatatca tccaggttga tatgcgtaac cacggtcttt caccgagaga tccggtaatg
     6601 aattacccgg cgatggcgca ggatcttgtt gataccctgg atgcacagca gatcgacaaa
     6661 gcaacattta tcggtcactc catgggcggt aaagcggtaa tggctcttac tgcactagcc
     6721 cccgatcgca ttgacaagct ggtggcgatt gatattgcgc cggtcgatta tcacgttcgc
     6781 cgtcatgatg agattttcgc ggccatcaac gcagtcagtg aatcagacgc acaaacacgc
     6841 cagcaagcag cggcaataat gcgccagcat cttaatgaag aaggggtgat tcagtttctg
     6901 ttgaaatctt ttgttgacgg ggagtggcgc tttaacgtgc cagtattgtg ggatcagtat
     6961 ccgcatattg tgggttggga gaaaatcccg gcatgggatc atcctaccct gtttatccct
     7021 ggcggcaatt ctccgtatgt tagcgagcag taccgtgatg atttactggc acaatttcca
     7081 caggctcggg cgcatgtgat tgcaggcgcg ggtcactggg tccatgctga aaaaccggat
     7141 gcggtattac gcgctatccg tcgctatctc aatgattaac gcaactgatt aaaaacccag
     7201 cgtccgcgtc gctttccgcg cgtggacgtt ggcgtgactc atcgcgctga tgtattatgg
     7261 cgcgctatcc atccgggcag gcgcctggct gattgtttcc cccgaagtca ccaagatcat
     7321 ggccaaagaa caaacggacc gtacgacatt agatctgttc gcgcacgagc gtcgaccggg
     7381 acgaccgaaa actaatccgc tttcgcgcga tgagcagctg cgtattaata aacgcaacca
     7441 actaaaacgc gacaaagtac gtggccttaa gcgtgtcgaa ctgaagctga acgcggaagc
     7501 tgtcgaggca ctgaacgagc tggcggaatc gcgcaatatg agccgtagcg agctgatcga
     7561 agagatgtta atgcagcaac tggcggcgct gcgtagtcag ggcatcgttt aaatttccac
     7621 tttcatgtag cacagtgtgc agtcctgctc gtttgctgat tccgcaagac tgcctgttct
     7681 gctatgattg cctttatccg tgggcaattt tccaccccca tttcaataag tttcaagagg
     7741 ttatttcact catggctatc actggcatct ttttcggcag cgacaccggt aataccgaaa
     7801 atatcgcaaa aatgattcaa aaacagcttg gtaaagacgt tgccgatgtc catgacattg
     7861 caaaaagcag caaagaagat ctggaagcat atgacattct gctgctgggc atcccaacct
     7921 ggtattacgg cgaagcgcag tgtgactggg atgacttctt cccgactctc gaagagattg
     7981 atttcaatgg taaactggtt gcgctgtttg gttgtggcga ccaggaagat tacgcagaat
     8041 acttctgcga tgcattaggc accatccgcg acatcattga accgcgcggc gcaactatcg
     8101 ttggtcactg gccaaccgcg ggctaccact tcgaagcgtc aaaaggtctg gctgatgatg
     8161 accactttgt cggtctggct atcgacgaag atcgtcagcc ggaactgact gctgaacgtg
     8221 tagaaaaatg ggttaaacaa atttctgaag agctgcatct cgacgaaatt ctcaatgcct
     8281 gatgtgatgc ggcgtagact catgtctacg ccgtattaat agataatgcc aatcaaaata
     8341 attgctacaa atttgtaact tttgctgttg tacctgtaca atgtcccggt gttcaagtgg
     8401 ccttgccgtt gtaaatgtaa gctgtgccac gtttttatta acaatatttg ccagggactt
     8461 gtggttttca tttaggcgtg gcaattctat aatgatacgc attatctcaa gagcaaattc
     8521 tgtcacttct cctaatgaag tgaaccgctt agtaacagga cagattccgc atgactgata
     8581 acaataccgc cctaaagaaa gctggcctga aagtaacgct tcctcgttta aaaatcctgg
     8641 aagttcttca ggagccggac aaccatcacg tcagtgcgga agatttatac aaacgtctga
     8701 tcgatatggg tgaagaaatt ggtctggcta cggtatatcg cgtactgaac cagtttgacg
     8761 atgctggtat cgtcacccgc cacaattttg aaggcggtaa atccgtattt gaactgacac
     8821 agcaacatca ccacgatcac ctgatctgcc tcgactgcgg caaggttatc gaatttagtg
     8881 atgattccat cgaagcgcgt cagcgtgaaa tcgccgcaaa acatggcatt cgcctgacta
     8941 accacagtct ctatctttac ggtcactgtg ccgaaggcga ttgccgcgaa gatgagcacg
     9001 cgcacgaagg caaataagcc agcctgaacg agaaaagcca acctgcaggt tggctttttt
     9061 atacaaggga aataagaaaa gcgagagtta cagctctcac ttatttgtta ttactgcgaa
     9121 tttcctgcca gaccttatca caatcggctt tcaccgcctg atcatttccg gtttgcgttg
     9181 cctgaataca ctgctgataa tccagtacgc ggacactttc ctgctcggca aactgctgat
     9241 gttgtttctc tttcttcagc acgtttaaca cgctctggca ggcttcaatt ttttccggcg
     9301 aaccttgcgc ggtgttgata caggcgctgt aagcctcttt cagacgactg tcttcctgcg
     9361 gtgcggttga ttgcgcacaa gctaccagcc ccgatgccat catggcgatg agaatcagtt
     9421 ttttcatagc aaattcctca aatgtgcggc aggcacaaaa cctgccgcgt cgggcatcag
     9481 aagatggtga atggtgcgat taccataaat ttcacgtcgc gctcatcctg gaagatgtta
     9541 ccgtaaccgc cgccccagct tgggatgtcg gagtggttgt cgtattcggt gaagtgcagt
     9601 ttgaacatcg tgcctttggc gcgaccgtcc tgaatggtgt acaccgcatc caggctgtag
     9661 gcagactctt caatagtccg gtttttgtcg tagtacgcat ccggattgct ctgccaggtc
     9721 gcaggtttag catcccatgc gtaaacgtag gaagcgccga tggcgaagcc tggaagattc
     9781 cagtttttca ggtcatacat cgcaccgaag aagaccgctt tttcgccgtt ggcgttgaag
     9841 tcagaacggt tatcccacca gatatccagg cgaccgttcg aggaagcgta ggttggagtc
     9901 atacgttgca ggaagtatcc ctgctgaccg tcggctttaa cccagctgcc ttccagacgc
     9961 aaatccacta catcagccgc ccggtaacca aaggtcaacg cttgcagcca ggcagtgccg
    10021 tcgtaaatgt cgttgacgct gcgatcgtca actttatcgc gcgtaccgta gaactggtag
    10081 ctggtggtta acgggctacc ggcgatatca aatttgtagc tggctttggc aaaatattga
    10141 tctatatacc cttccgcctg accaaatgcc gcttccagta caaagttatt tttgaagtcg
    10201 tacttcgcac cgatggagtg cagataatca actttggtag ttttatcgtt ctgataaaac
    10261 tcatccattt cgagatgcca cggtgctttg tattcgttgg tccacatgta ggagaaactc
    10321 aacgcaccag catcgccgta atcaaaattc gccccggctt ccgcaccctg ataagtaccc
    10381 ggcataaagc tccagtgcgg cgctaacagc gtctgaccag ttggctgaat gtaacctgcc
    10441 ctcgcccaca ccggaccgta tttaaatttg gccgctgctt tatacaggct tataccgctt
    10501 ttgtcgccgg accagtcttc gtcataggct ttattgcttt ttgaaaacgc gatttcgttc
    10561 gggtgagagc tgtcaccgtt ttccgccatt tcaatcgccg taaacgcagc aatatcaagg
    10621 ccgaacatat cagcagcata accggactga aaatcgaggt tggcattcca ggtggagtga
    10681 gaaaggttgg ttttgtattt gtcgccgtcg gtaacatctt tacggtcgcg ttcacgctgc
    10741 cagtaataga taccgccggt taaggttgaa tcgtcgatga atccttcggc gcgagcctcc
    10801 ggcattgcca taaagcccga cattgctgta acaccggcga tagccagcgc cagcgtacta
    10861 cgtttgccac taaacgtacg catgggttaa tcctctttga cgtataaatt gctgcaccaa
    10921 aggtgaacag cgaaaaaaat aaaaattaaa aaagtagcgt tgagaaaagt ctcgctgact
    10981 acaggaacag actatttttc gcgacgcgaa ttatcaatct ctttctgtaa ccagaatgta
    11041 agaacatgag caaccgcaca tattttgatg atttttgtta caaggcttaa gtttgcagtt
    11101 gaaaggcttg cagaagaata aaagcgtgaa tttccagtaa gatgaaaacg gttacatttt
    11161 atttaattat ggtttgtgat tttaaatttg ttttatggat aaattgcgaa gcggaattaa
    11221 ttcgcgaaga aaagcagaaa aaaacgccgc agagcgcggc gtttgggatt gtttgcatat
    11281 ttcttgccgg atgtggcgta aatgcctcac ccggcctggt agaacttaaa actactcgcc
    11341 tactttcgcc caggtatcac gcagccctac ggtgcggtta aataccggtt tttcggcagt
    11401 ggaatggcgg ctgtcgaggc agaaataacc ttcacgctca aactggaatg ctttacccgc
    11461 taccgcgtct ttcagcgacg gttcagcgaa accctgtttg atcaccagcg attccgggtt
    11521 aatcaccgac aggaaatcat ccgcagcacc tgggttaggc acgctgaaca gacggtcata
    11581 caaacggatt tcaaccggca gcgcatgtgc cgcgctcacc cagtgaataa cacctttcac
    11641 tttacgacca tctgccggat ctttgcttaa ggtgtcggcg tcataagtac agaagatggt
    11701 ggtgatatta ccttcggcat ctttctcgac gcgttctgcc ttaatcacgt aagcattacg
    11761 cagacgcact tctttaccca acaccagacg tttgtactgc ttgttagctt cttcgcggaa
    11821 atcggcgcga tcgatccaaa tctcacagct aaacggcacc tgacggctac ccatttccgg
    11881 tttgttcgga tggttcggca tggtgaccat ttcgccttcg ccctggtagt tttcgataac
    11941 cagtttcacc ggatcgataa ccgccattgc gcgcggggca ttttcgttga gatcttcacg
    12001 gatgcaggat tccagtgacg ccatctcaat ggtgttgtcc tgcttggtca caccaatgcg
    12061 tttgcagaac tcacgaatag aagccgcagt gtaaccacga cgacgcagac cagaaatggt
    12121 cggcatacgc gggtcatccc agccttcaac gtgcttatcg gtcaccagca ggttcagctt
    12181 acgcttggac atcacagtgt attccagatt caggcgcgag aattcgtact ggcgcgggtg
    12241 aacaggaatg gtgatgttgt ccagtaccca gtcgtacaga cggcggttgt cctggaactc
    12301 aagcgtacac agagagtgcg taataccttc cagcgcatcg ctgatgcagt gggtgaagtc
    12361 gtacatcggg tagatgcacc acttgttgcc agtctggtgg tgttcagcaa atttaatgcg
    12421 gtacagcacc ggatcgcgca ttacgataaa tggcgacgcc atgtcgattt tcgcacgcag
    12481 gcaggcttta ccttcttcaa aactaccggt acgcattttt tcgaacagcg ccaggttctc
    12541 ttcaacgctg cggtcgcggt acgggctgtt tttacccggc tgcgtcaggg tgccgcggta
    12601 ttcgcggatc tgttccggtg tcagttcatc aacgtacgcc aggcctttat tgatcagttc
    12661 gatcgcatag gcgtggagct gatcaaaata atcggaggag taacggacgt taccagacca
    12721 gtgaaaacct aaccactcaa cgtcgttttt gatcgactca acatactcga tatcttcttt
    12781 taccgggtta gtgtcgtcga aacgcaggtt gcactggcct ttatagtcct gggcgatccc
    12841 gaagttcagg cagatagatt tcgcatggcc aatatgcaga tagccattcg gctccggcgg
    12901 gaaacgggtg tgtactgtgg tgtgcttacc actggccaga tcttcatcga tgatctgacg
    12961 aataaagtta gtcgggcggg cttctgcctc actcatcgtg gattcctcaa agcgtaaaca
    13021 acgtataacg gcgtatgatc ttataagcgg gacaggctga caactgttag ttagtgaaaa
    13081 tcgaaaatct cactgagatt atggggatga ttgatgaaaa aaaaagcggc agagaagatc
    13141 tccaccgctt gtcattgttg gatgcgacgc tcaagcgtcg catcaggcat aaagcagatt
    13201 actttttgat ttcatacagc ggtgtttgac ccgccacaac atggccctga gctttaatga
    13261 tcaggccgct gaaatcgtcg atattgctgc aaaccaccgg gctaatcatc gagcgggcgt
    13321 tagcgttcag gtaatccaga tccatttcca gaatcggttg tcctgcgctt acctgcgcgc
    13381 cctcttccac cagacgttta aagcctttac cttccagcgc tacggtgtcg atacccatat
    13441 ggacgacgat ctccgcgcct ttttcggttt ccagacagaa cgcgtggttg gtgttgaaga
    13501 ttttcacgat tgtccctgcg gctggtgata cgacgatttt atctgtcggt ttcaccgcca
    13561 caccgtcacc caccgctttg ctggcgaatg cttcgtcagg aacctgatcc agtgccacaa
    13621 catcaccggt aatcggcgat accagctccg cgatagacac cgcgtttggt acagcctgcg
    13681 gttttgctac aggcgcggca gttgcaggcg cagtttcagc tgacgcagcg gctaccggac
    13741 cacgggcaac gactttcttc atcgcatcgc cgatggattc tgctttcgcg cccacaatca
    13801 cctgaatagt ctgtttgttc agttttacta ccccagaagc ccccagacgt ttacacatca
    13861 catcgttgac gcgagctgag tcaaccacgg taaggcgcag acgagtaata caggcgtcaa
    13921 tggctttcag gttgtcagta ccgccaaccg cagcaatata gttggttgcc agttgattga
    13981 gaccttcttc cgtgttgctg ttggcttctt cagtaacgat ctcgtcttct ttatcttcac
    14041 ggcccggcgt tttcaggttg aacatgcgga taaccaaact gaacaccacg aagtagatag
    14101 cgaagaagac aacacccatc accagcagca tccagacgtt ctggctggcg gccggcaggt
    14161 tatacatcaa cgcgtagtcg atagcccccg cagagaagga gaagcccgcg tggataccca
    14221 gcagcgttgc cacaaacagg ctgataccgg tcagcagtgc gtgcaggagg tacagcagcg
    14281 gcgcaaggaa catgaacagg aattccagcg gctcagtcac accggtcagg aacgcagtaa
    14341 cagcaacaga aagcagcata ccgccaacca tcggacgacg ctctttcggt gctgcaaagt
    14401 acatcgccag cgccgcaccc ggcagaccga acatcatgat cgggaagaag ccggacatga
    14461 acatccccgc ggtgccgtca ccggcataga agcggttaat gtcgccgtgg aaaaccgtac
    14521 ccgccgcgtt ggtgaattca ccaatctgga accaggcgat ggtgttcagt acctgatgca
    14581 gaccggttgg gatcagcaga cggttgatga aaccaaagat accggaaccc agcgcgcccg
    14641 cagaaacgat ccactcgccg cctgcatgga tagcgtgctg taccggcggc cagacgtaac
    14701 caaaaatggc cgccagcacc aggcagaaga agccggtggc aatcggcaca aagcgtttgc
    14761 cgccgaagaa gctcaggaag tccggcagtt taatatcgga ccaacggtta taggctgcac
    14821 caccgaccag accggtaatg atacccgcca gtacacccat gttaatttct gggttgatgg
    14881 tcaccatcgc tttggttaac acaaagtaac ctaccgcacc cgccagtgcc gccgcacctg
    14941 cgctgtcttt cgaccagctg gatgccacgc cgatggcgaa gattaatgcg aggttatcaa
    15001 aaatcgcacc gcccgcctgg gcaataaacg caacgttaag taaatctggc tgaccgaatc
    15061 gcagcaacag cgccgccacc ggcagcaccg cgatagggag ctgtaacgcc ctacctagtc
    15121 gctggaaaaa acctaaaata ttcatcttat tccccctacg agaaccctat ttggctcgtt
    15181 taaagccgta tttttatttt gctgcaaatt gtactgccga tgttctgtaa tcagattgtt
    15241 agatcatctg ctacagagtg tgtgaaaatt taattcgtat cgcaaattaa acgcgtgtct
    15301 tttgtgagtt ttgtcaccaa atatcgttat catcactccc ttttactggc taaaccagaa
    15361 aacttatttt atcattcaaa aaatcaggtc ggattgacgc ctgtctgcgc aaatccaggt
    15421 tacgcttaaa gatgcctaat ccgccaacgg cttacatttt acttattgag gtgaataatg
    15481 agactgatcc ccctgactac cgctgaacag gtcggcaaat gggctgctcg ccatatcgtc
    15541 aatcgtatca atgcgttcaa accgactgcc gatcgtccgt ttgtactggg cctgccgact
    15601 ggcggcacgc cgatgaccac ctataaagcg ttagtcgaaa tgcataaagc aggccaggtc
    15661 agctttaagc acgttgtcac cttcaacatg gacgaatatg tcggtctgcc gaaagagcat
    15721 ccggaaagct actacagctt tatgcaccgt aatttcttcg atcacgttga tattccagca
    15781 gaaaatatca accttctcaa cggcaacgcc ccggatatcg acgccgaatg ccgccagtat
    15841 gaagaaaaaa tccgttctta cggaaaaatt catctgttta tgggcggtgt aggtaacgac
    15901 ggtcatattg catttaacga accggcgtct tctctggctt ctcgtactcg tatcaaaacc
    15961 ctgactcatg acactcgcgt cgcgaactct cgtttctttg ataacgatgt taatcaggtg
    16021 ccaaaatatg ccctgactgt cggtgttggt acattgctgg atgccgaaga agtgatgatt
    16081 ctggtgctgg gtagccagaa agcactggca ctgcaggccg ccgttgaagg ttgcgtgaac
    16141 cacatgtgga ccatcagctg tctgcaactg catccaaaag cgatcatggt gtgcgatgaa
    16201 ccttccacca tggagctgaa agttaagact ttaagatatt tcaatgaatt agaagcagaa
    16261 aatatcaaag gtctgtaatt attatccctg ccctggctcc ttgctcaggg caatattttt
    16321 taaaatcggg ggtcagaatg tatgcattaa cccagggccg gatctttacc ggccacgaat
    16381 ttcttgatga ccacgcggtt gttatcgctg atggcctgat taaaagcgtc tgtccggtag
    16441 cggaactgcc gccagagatc gaacaacgtt cactgaacgg ggccattctc tcccccggtt
    16501 ttatcgatgt gcagttaaac ggctgcggcg gcgtgcagtt taacgatact gctgaagcag
    16561 tcagcgtaga aacgctggaa atcatgcaga aagccaatga gaaatcaggc tgtactaact
    16621 atttgccgac gcttatcacc accagcgacg agctgatgaa acagggcgtg cgcgttatgc
    16681 gcgagtacct ggcaaaacat ccgaatcagg cgttaggtct gcatctggaa ggtccgtggc
    16741 tgaatctggt aaaaaaaggc acccataatc cgaattttgt gcgtaagcct gatgccgcgc
    16801 tggtcgattt cctgtgtgag aacgccgacg tgattaccaa agtgactctg gcaccggaaa
    16861 tggttccagc ggaagtcatc agcaaactgg caaatgccgg gattgtggtt tctgctggtc
    16921 actccaacgc gacgttgaaa gaagcgaaag ccggtttccg cgcggggatt acctttgcca
    16981 cccatctgta caacgcgatg ccgtatatta ccggtcgtga accgggcctg gcgggcgcga
    17041 tcctcgacga agctgacatt tattgcggta ttattgctga tggcctgcat gttgattacg
    17101 ccaacattcg taacgctaag cgtctgaaag gcgacaaatt gtgtctggtt accgatgcca
    17161 ccgcgccagc aggtgccaac attgaacagt tcatttttgc gggtaaaaca atatactacc
    17221 gtaatggact ttgtgtggat gagaacggta cgttaagcgg ttcatcctta accatgattg
    17281 aaggcgtgcg taatctggtc gaacattgtg gtatcgcact ggatgaagtg ctgcgtatgg
    17341 cgacgctcta tccggcacgt gcgattggcg ttgagaaacg tctcggcacg ctcgccgcag
    17401 gtaaagtagc caacctgact gcattcacac ctgattttaa aatcaccagg accatcgtta
    17461 acggtaacga ggtcgtaact caataagaga aagtatgaca ccaggcggac aagctcagat
    17521 aggtaatgtt gatctcgtaa aacagcttaa cagcgcggcg gtttatcgcc tgattgacca
    17581 gtacgggcca atctcgcgga ttcagattgc cgagcaaagt cagcttgccc ccgccagcgt
    17641 aaccaaaatt acgcgtcagc ttatcgaacg cgggctgatc aaagaagttg atcagcaggc
    17701 ctccaccggg ggccgccgcg ctatctccat cgtcaccgaa acccgcaatt tccacgcaat
    17761 cggcgtacgg cttggtcgtc acgacgccac catcaccttg tttgatctca gcagcaaagt
    17821 gctggcagaa gaacattacc cgctgccgga acgtacccag cagacgctgg aacatgccct
    17881 gctgaatgcc attgctcagt ttattgatag ctaccagcgc aagctacgcg agctgatcgc
    17941 gatttcggtg atcctgccag ggcttgtaga cccggacagc ggcaaaattc attatatgcc
    18001 gcatattcag gtagaaaact gggggctggt tgaagcactg gaagagcgtt ttaaagtgac
    18061 ctgttttgtc ggccacgata tccgcagtct ggcgctggca gagcactact tcggtgcaag
    18121 tcaggattgc gaagactcca ttctggtgcg tgtccatcgc ggaaccgggg ccgggattat
    18181 ctctaacggg cgaattttta tcgggcgcaa cggcaacgtc ggtgaaattg gccatattca
    18241 ggtcgaaccg ctgggtgaac gctgccactg cggcaacttt ggctgcctgg aaactatcgc
    18301 cgccaatgct gccattgaac aacgggtgtt gaatctgtta aagcagggct accagagccg
    18361 tgtgccactg gacgactgca ccatcaaaac tatctgcaaa gccgcgaaca aaggcgatag
    18421 cctggcctcg gaagtgattg aatatgtcgg tcgtcatctg ggtaaaacca tcgccattgc
    18481 tatcaactta tttaatccgc aaaaaattgt tattgctgga gaaatcaccg aagccgataa
    18541 agtgctgctc cctgctattg aaagctgcat taatacccag gcgctgaagg cgtttcgtac
    18601 taatctaccg gtggtacgtt ctgagttgga tcaccgctca gcaatcggcg cttttgcgct
    18661 ggtaaaacgc gccatgctca acggtatttt gctccagcat ttgctggaaa attaatgtgc
    18721 ttttatagtg gcgcttattg ttgtcaatat tctgggtagt ccatgaccat taaaaatgta
    18781 atttgcgata tcgacggcgt gctgatgcac gataacgtcg ccgtaccggg tgcagcggaa
    18841 tttttgcacg ggattatgga taaaggcctg ccgctggtgt tgctgaccaa ctatccttcg
    18901 cagaccgggc aagatctggc gaatcgcttt gccactgccg gtgtcgatgt accggatagc
    18961 gtgttttata cctccgcgat ggcgaccgcc gatttcctgc gtcgccagga aggcaagaaa
    19021 gcgtatgtgg tgggcgaagg cgcactgatt catgaactgt acaaagccgg tttcactatt
    19081 accgatgtga accctgattt tgtgattgtt ggcgaaacgc gttcctacaa ctgggacatg
    19141 atgcataaag cagcctattt cgtcgctaac ggcgcacgct ttatcgccac caacccggac
    19201 acccacgggc gcggttttta tcccgcctgt ggcgcgttgt gtgcagggat cgagaaaatc
    19261 tccgggcgca aaccgttcta tgttggaaag ccgagtccgt ggatcatccg cgcggcatta
    19321 aacaaaatgc aggcgcattc ggaagaaacg gtgattgtcg gcgataactt gcgtaccgat
    19381 attctggcgg gcttccaggc cggtctggag acgattctgg tgctttctgg tgtttcgtca
    19441 ctcgacgata tcgacagtat gccgttccgc ccaagttgga tttacccatc ggtcgctgaa
    19501 atcgacgtta tctgaatagc aaccacgcct cagggcgtgg tttcttattt ttacccatca
    19561 ttaaaatcac cccattcaat aaaacccgtc aaaaattatc gatccatcaa taaatatacc
    19621 caaaagccct cccctttttc atcaatcagc aatcgccatt acgtttttta tttttccact
    19681 gccaaatcgc ttgcgcaccg tgctgctttg cggcattttt ttaacaagta aacacaacac
    19741 gcagtaaaca ccaggttaac ggagaaggtt atgtgttcaa tttttggcgt attcgatatc
    19801 aaaacagacg cagttgagct gcgtaagaaa gcactcgagc tgtcacgcct gatgcgtcat
    19861 cgtggcccgg actggtccgg tatttatgcc agcgataacg ccatcctcgc ccacgaacgt
    19921 ctgtcaattg ttgacgttaa cgcgggggcg caacctctct acaaccaaca aaaaactcat
    19981 gtgctggcag taaacggtga aatctacaac caccaggcat tgcgcgctga atatggcgat
    20041 cgttaccagt tccagaccgg gtctgactgt gaagtgatcc tcgcgctgta tcaggaaaaa
    20101 gggccggaat ttctcgacga cttgcagggc atgtttgcct ttgccctgta cgacagcgaa
    20161 aaagatgcat atctgattgg tcgcgaccat ctggggatca tcccactgta tatgggctat
    20221 gacgaacacg gtcagctgta tgtggcctca gaaatgaaag ccctcgtgcc agtttgccgc
    20281 acgattaaag agtttccggc ggggagctat ttgtggagtc aggacggcga aatccgttct
    20341 tattatcatc gcgactggtt cgactacgat gcggtgaaag ataacgtgac cgacaaaaac
    20401 gagctgcgtc aggcactgga agattccgtt aaaagccatc tgatgtctga tgtgccttac
    20461 ggtgtgctgc tttctggtgg tctggattcc tcaattattt ccgctatcac caagaaatac
    20521 gccgcccgtc gcgtggaaga tcaggaacgc tctgaagcct ggtggccgca gttgcactcc
    20581 tttgctgtag gtctgccggg ctcaccggat ctgaaagcag cccaggaagt ggcaaaccat
    20641 ctgggcacgg tgcatcacga aattcacttc actgtacagg aaggtctgga tgccatccgc
    20701 gacgtgattt accacatcga aacttatgat gtgaccacta ttcgcgcttc aacaccgatg
    20761 tatttaatgt cgcgtaagat caaggcgatg ggcattaaaa tggtgctgtc cggtgaaggt
    20821 tctgacgaag tgtttggcgg ttatctatac ttccacaaag cgccgaacgc taaagagttg
    20881 catgaagaga cggtgcgtaa actgctggcc ctgcatatgt atgactgcgc gcgtgccaac
    20941 aaagcgatgt cagcctgggg cgtggaagca cgcgttccgt tcctcgacaa aaaattcctt
    21001 gatgtggcga tgcgcattaa cccacaggat aaaatgtgcg gtaacggcaa aatggaaaaa
    21061 cacatcctgc gtgaatgttt tgaagcgtat ctgcctgcaa gcgtggcctg gcggcaaaaa
    21121 gagcagttct ccgatggcgt cggttacagt tggatcgaca ccctgaaaga agtggcagcg
    21181 cagcaggtgt ctgatcaaca gctggaaact gcccgcttcc gcttcccgta caacacgccg
    21241 acctcaaaag aagcgtatct gtaccgggag atctttgaag aattgttccc gcttccgagc
    21301 gccgctgagt gcgtgccggg cggtccttcc gtcgcttgtt cttccgctaa agcgattgag
    21361 tgggatgaag cgttcaagaa aatggacgat ccgtctggtc gcgcggttgg cgttcaccag
    21421 tcggcatata aataagacgt gtaatattgc cccggagaaa tccggggcaa tttcagttac
    21481 tggggcgctt gttgattctg atatgcagta aggaaatgtt ccgggtttgc cacatcgctg
    21541 tttgacaact cattactgta ggccagtatt tcatagatta atttcccacc actattgttg
    21601 aattccgaat ttcatcctgt atgacaaaag aatggctgta gggtggggaa aaaacacaaa
    21661 gtaaacaata attgacgaat atagcgccat gctgttcgca acctaaccaa acagtcactt
    21721 tcgagcaatt ttccttgaaa aagaggttga cgctgcaagg ctctatacgc ataatgcgcc
    21781 ccgcaacgcc gataaggtat cgcgaaaaaa aagatggcta cgtagctcag ttggttagag
    21841 cacatcactc ataatgatgg ggtcacaggt tcgaatcccg tcgtagccac catctttttt
    21901 gcgggagtgg cgaaattggt agacgcacca gatttaggtt ctggcgccgc aaggtgtgcg
    21961 agttcaagtc tcgcctcccg caccattcac cagaaagcgt tgtacggatg gggtatcgcc
    22021 aagcggtaag gcaccggttt ttgataccgg cattccctgg ttcgaatcca ggtaccccag
    22081 ccatcgaaga aacaatctgg ctacgtagct cagttggtta gagcacatca ctcataatga
    22141 tggggtcaca ggttcgaatc ccgtcgtagc caccaaattc tgaatgtgtc gaatatgttc
    22201 ggcaaattca aaaaccaatt tgttggggta tcgccaagcg gtaaggcacc ggattctgat
    22261 tccggcattc cgaggttcga atcctcgtac cccagccaca ttaaaaaagc tcgcctcggc
    22321 gagctttttg cttttctgcg tatattcaat gccgaatgcg atgttgactc gtcttatcct
    22381 tcaaagtcgg atgcgacgct gccgcgtctt atccgaccta cggttggcat gcatccagca
    22441 atgttgtgag gctacaaccc taacgcatat ttcagcgcct tacgtttcaa cacgccagca
    22501 cgctccgccg ccatcaaacc aagattacgc acaaaacgca gcggtggcag attattgctg
    22561 aatccggcat aaaacagatc cataccactt tgcatcataa agttatccgc catacgccgc
    22621 atctggtaac gcttgagcac aggataactg gcccacgctt cgccgtagct gcgggcgttc
    22681 accagaacgt caatcagggc atcgacatca cgataaccaa gattcacccc ctgccccgcc
    22741 agcggatgga tggtatgcgc ggcatcgccc accagagcaa gccctggttg cacatactgc
    22801 aacgcatggc gacgtatcag cggaaacgca ccagcggcaa gcggtgtcac gtaacccaga
    22861 cgcgacggga aatgcttcgc gatttccgcc tgtagctgcg ccatattcat attctgcaac
    22921 tggcgaatgc gcgccggaga gtcataccac accagcgatg cccagttatc aaacaacggc
    22981 agaaacgcac gcggtccgtc cggggtaaat tgctgccagg tgctgtcgcc gggatcgttc
    23041 tcacactgta cgctaatcaa catacacgac tgcgcatact gccaggcatg aacgccaatt
    23101 cccgccatct gtcgcacctg cgaatttgcg ccgtcggcac caatcaccag cttcgcgagg
    23161 atcgtttcac cgcccttcag ttccagctcc tgaagatcat tatggcgatg cagtgcaatc
    23221 agcgaggttg gcacacgtaa cgttactttc ggatgcgctt ccagcgcctg ccacaacgcc
    23281 tgttgcagga cagtgttttc caccatgtag ccaagcaacg gtagcttaag ttcagcggcg
    23341 tcaaacacca catgcgccgt ttcccactcc cacgtttcca gtctgcggta aggatggcaa
    23401 cgcatagcct gtactgcatc ccagacccct aaccctttaa gcaatgatac cgaagccgca
    23461 ctaatcgccg agatccgcac gtccggctgg ctgtcagcga caaacggtgc aggttgggca
    23521 tgttcgataa ccattaccga aaatccgtgc tgagccagtc ccagcgccag tgcgccgccg
    23581 accattcctc cgccgacaat ggcaatttcc gttggttgat ttgtcatggt caatcatcct
    23641 gttagcaata tgctttaagt ttacaggatt tttaccccca agggctgata acgctcatac
    23701 tggtcacaac ggcagcaaag cattacacta tgcgccctgc attcctggct actatttcgc
    23761 aagagcaagt cgatgaccaa aaaactccat attaaaacct ggggctgtca gatgaacgag
    23821 tacgattcat cgaagatggc cgatctgctg gatgccaccc acggctatca actgaccgac
    23881 gtggcggaag aagcggatgt gctgctgctg aacacctgct caatccgcga gaaggctcag
    23941 gaaaaagtct tccatcagtt gggtcgctgg aaactgttaa aagagaagaa tccagacctg
    24001 attatcggcg tcggcggctg cgtggcatcg caagaaggcg agcacattcg ccagcgcgcc
    24061 cactatgtcg atattatttt tgggccgcaa acgctgcacc gtctgccgga gatgatcaac
    24121 tccgtgcgcg gcgaccgcag cccggttgta gatatcagct tcccggaaat cgagaagttt
    24181 gaccgtctgc cggaaccgcg cgccgaaggg ccgaccgcgt ttgtctccat catggaaggc
    24241 tgcaataaat attgcaccta ctgcgtagtg ccttacaccc gtggtgaaga ggtaagccgt
    24301 ccgtccgacg atattctgtt tgagattgcc cagcttgctg ctcagggcgt gcgtgaagtt
    24361 aacctgctcg gccagaacgt gaacgcctgg cgcggtgaga actacgacgg caccaccgga
    24421 tcgtttgccg atctgctgcg tctggttgcg gcgatcgacg ggatcgatcg tattcgcttt
    24481 accaccagcc atccgatcga attcaccgac gatatcatcg aagtgtaccg cgacacgccg
    24541 gagctggtga gcttcctgca tctgccggta cagagcggtt ccgatcgcat tctgaacctg
    24601 atgggacgta cccatacagc gctggagtac aaagcgatca tccgtaagct gcgtgcggcg
    24661 cgtccggata ttcagatcag ttctgacttc atcgttggct tccctggcga aaccaccgaa
    24721 gacttcgaga aaacgatgaa gctgattgcc gacgtcaatt tcgacatgag ctacagcttt
    24781 atcttctccg ctcgtccagg tacaccagcc gccgatatgg ttgatgatgt tccggaagaa
    24841 gagaagaagc agcgcctgta tattctacaa gaacgtatca atcagcaagc aatggcatgg
    24901 agccgccgga tgctcggtac cacccagcgt attctggtag aaggtacatc gcgtaagagc
    24961 atcatggagc tttccggccg tactgaaaat aaccgcgtcg tcaacttcga aggtacgccg
    25021 gatatgatcg gtaaattcgt cgatgtagaa attaccgacg tctacccgaa ctctctgcgc
    25081 ggtaaagtgg tgcgaactga agatgaaatg ggtctgcgcg tggcagaaac accggaatcg
    25141 gtgattgccc gtacccgcaa agaaaacgac cttggcgtgg gttattatca gccgtaattc
    25201 tcaggcccgc cgttccggtg ggccttgttt tttctctctt tatccccatc tttatcgcaa
    25261 tgcttgcgcc tttttcctgt ggcgtgaata atttccttat aggccggtga atgttcagct
    25321 tccggcggca tacagataag aggaacggtt tgaacataga cactcgcgaa atcaccctgg
    25381 agccagcaga caatgcgcgt ctgttgagcc tgtgcggccc gtttgatgac aacatcaaac
    25441 aactcgaacg ccgtctcggc atcgaaatca atcgccgcga taaccacttt aaactgaccg
    25501 gccgtccgat ttgcgtcacc gctgcggcag acattctgcg tagcctgtat gtcgatactg
    25561 ccccgatgcg cggtcagatt caggatatcg aaccggaaca gatccacctt gcgattaaag
    25621 aagcgcgggt actggagcaa agcgcggaga gcgtgccgga gtacggcaaa gcggtcaata
    25681 tcaaaaccaa acgcggcgta attaagccgc gcacgccaaa ccaggcgcag tacatcgcca
    25741 atattctcga ccatgacatt accttcggcg ttggcccggc gggtacgggt aaaacctacc
    25801 tggcagtggc tgcggcagtt gatgccctgg agcgtcagga aattcgccgt attctgctga
    25861 ctcgtccggc agtagaagcc ggtgagaaac tgggcttcct gcctggcgat ttaagccaga
    25921 aagtagaccc gtatctgcgc ccgctgtacg acgcgctgtt tgaaatgctg ggctttgaga
    25981 aagtcgagaa actgattgag cgcaacgtta ttgaagtcgc accgctggcc tatatgcgtg
    26041 gtcgtacgct gaacgacgcg tttatcattc ttgatgagag ccagaacacc accatcgaac
    26101 agatgaagat gttcctgacc cgtatcggtt ttaactcaaa agcggttatc accggcgacg
    26161 tcacacagat cgacctgccg cgtaatacca agtctggttt gcgtcatgcc atcgaagtac
    26221 tggctgatgt cgaagagatc agctttaact tcttccacag cgaagacgtg gttcgtcacc
    26281 cggtggtggc gcgtatcgtt aacgcctatg aagcctggga agaagccgaa caaaaacgta
    26341 aagcggcgct ggcagcagaa cgcaagcgcg aagaacagga acaaaaatga gtcaggtgat
    26401 cctcgattta caactggcat gtgaagataa ttccgggtta ccggaagaga gccagtttca
    26461 gacatggctg aatgcggtga tcccgcagtt tcaggaagaa tcggaagtga ctattcgcgt
    26521 ggtcgatacc gccgaaagcc acagtctgaa tctgacctat cgcggtaagg ataagccgac
    26581 caacgtgctc tccttcccgt ttgaagtacc gcctggcatg gaaatgtcgc tactgggcga
    26641 tctggttatc tgccgtcagg tggttgagaa ggaagctcag gagcaaggca aaccactgga
    26701 ggcgcactgg gcgcatatgg tggtgcacgg cagtctgcat ttgttaggtt acgatcacat
    26761 cgaagatgac gaagcagaag aaatggaagc catcgaaaca gagattatgc ttgctctggg
    26821 ctatgaggat ccgtacattg ccgagaaaga ataatttgcc aggcatttga ctggcagacg
    26881 tccccgccgt tggtgattaa cgacggcgtt ttgattaact aacatgagaa cccatacgac
    26941 gccatgagcg acgacaattc acacagtagt gacacgataa gcaacaagaa gggatttttc
    27001 tccctgttac tcagccaact tttccacggt gaaccgaaaa accgtgacga actgctggcg
    27061 ctgatccgtg attccgggca gaacgacctt atcgacgaag atacgcgcga tatgctcgaa
    27121 ggggtgatgg acatcgcaga ccaacgcgtt cgcgacatca tgatcccccg ctcccagatg
    27181 attaccctga aacgcaacca gacgctggac gaatgccttg atgtcatcat cgagtccgcc
    27241 cactcacgtt tcccggtgat tagcgaagac aaagatcaca ttgaagggat tctgatggcg
    27301 aaagacttgc tgccgtttat gcgcagcgat gctgaagcct tcagcatgga caaagtgtta
    27361 cgtcaggcgg ttgtcgttcc tgaaagtaag cgcgtagacc ggatgctgaa agagtttcgc
    27421 tctcagcgtt accacatggc gatcgtcatt gacgaattcg gtggggtttc cggtctggtg
    27481 accattgaag acatcctgga actgattgtt ggtgagattg aagacgagta tgacgaagaa
    27541 gatgatatcg acttccgtca gctgagtcgt catacctgga ccgtgcgcgc actggcttct
    27601 attgaagact tcaacgaagc gttcggcacc cactttagcg atgaagaggt tgacactatc
    27661 ggtggtctgg tgatgcaggc atttgggcat cttccggcgc gtggcgaaac catcgacatc
    27721 gacggttacc agttcaaagt ggcgatggcc gacagtcggc gtattattca ggttcatgtc
    27781 aaaatcccgg atgactcacc ccagccgaag ctggatgaat aaaaccgaaa ctggatagat
    27841 aactacatgg cttttgcctc attaattgaa cgccagcgca ttcgcctgct gctggcgtta
    27901 ttattcggtg cctgcggaac gctggccttc tctccttacg acgtctggcc tgcggcgatt
    27961 atttcgctga tggggcttca ggcgttgacc tttaaccgcc gtccactcca gtctgccgct
    28021 attggctttt gctggggatt tggcctcttt ggcagcggta ttaactgggt ctatgtcagc
    28081 atcgcgacct ttggcggaat gcctggcccg gttaacatct tcctggtggt actgctggcg
    28141 gcgtatttgt cgctgtatac cggactgttt gctggcgtgc tgtcgcgtct gtggccgaaa
    28201 accacctggc tgcgcgtggc gattgccgcc cctgctctct ggcaagtgac cgagtttctg
    28261 cgcggttggg tactaacagg cttcccgtgg ttacagttcg gctatagcca gattgatggc
    28321 ccgttaaaag ggctggcacc gataatgggc gtggaagcca ttaacttcct gctgatgatg
    28381 gttagcggcc tgctggcact ggcgttggtc aaacgcaact ggcgtccgct ggtggtggcc
    28441 gtcgtgctgt ttgcccttcc cttcccgctg cgttacatcc agtggtttac cccgcaaccg
    28501 gaaaaaacta ttcaggtttc gatggttcag ggcgatattc cgcaatcgct gaaatgggac
    28561 ggagaccagc tacttaatac gctgaagata tactacaacg caacggcacc gctgatgggc
    28621 aaatcatcgt tgattatctg gccggagtcg gcgataaccg atctggaaat taatcagcaa
    28681 ccgttcctca aagctctgga cggtgagttg cgcgataaag ggagctcgct ggtcaccggg
    28741 attgtcgacg cgcgtctcaa taaacagaac cgctacgata cctacaacac catcatcaca
    28801 ctgggtaaag gcgcaccgta cagctatgaa tcagccgatc gctataacaa aaaccatctg
    28861 gtgccgtttg gcgagtttgt cccactggag tcgatactgc gtccgttagc accgttcttt
    28921 gatttgccga tgtcttcatt cagccgcgga ccatatattc agccaccgct gtcgttgaat
    28981 ggcattcaac ttactgcggc catttgctac gaaatcattc tcggcgagca agtgcgcgat
    29041 aacttccgcc cggataccga ctatctgctg actatctcca acgatgcgtg gtttggtaag
    29101 tctattggtc catggcagca cttccagatg gcgcgaatgc gtgcgctgga gctggcgcgc
    29161 ccactgttgc gcagcaccaa caacggcatt acggcggtga ttggcccgca gggtgagatt
    29221 caggcgatga tcccgcagtt cacccgcgag gtgttaacca ctaacgtgac gccgactacc
    29281 ggactcacac cgtacgcacg taccggcaac tggccgctgt gggtgctgac ggcactgttt
    29341 ggttttgcag ctgtgctgat gagtctgcga gcacgtaaac attaatgtgc ctgatgtatc
    29401 cggcatgaac aaaacatact ttctcattga gttgtggggc atgattatca tgcccttttt
    29461 tcttacggtt cagggataca agccacatcg ccatattggg gatgccgaat aattgcctgc
    29521 ggatcaagcg agcgcgtcct gtccatttgg attaacctgt tcttcgccat aacaaatatc
    29581 tttctaccat ccgctgaaaa acgaaggtga ttaacgtagc tggcaggata aacgccaata
    29641 atctgcccgg cattaatatt gagcaggatg acataacctt gcaggccata tgacaccaac
    29701 gctaaatatt gaccgtccgg gctaataccc tcaacggaat aaacgctcct gctacaccat
    29761 tgcgtcataa tgctggcttg ttgatcccct acgcttatac gtgaaatccc ctggctatcg
    29821 gcaaaaataa tctcgccatt ttttggtcgg aatcccatcg cagaaacgat accacgcgca
    29881 tcacctgtgg gaaagtcttc tttctgatgc gtgatggtat tgatgcgtac aatgtcgcct
    29941 ctgaccatac cgggtatcaa caatgttttt tcatccgggg aaagtgccat aacccaggcg
    30001 cctggtttac cgctcgtccc cgtcccgtcg tatttctcca gcgggatatc cgtcacatgc
    30061 tgataagtca ccagatcaat ggctgacaca ctattactgg tgcgattcat cacccaggct
    30121 tttgtgccat cacgcgagag gataaggctg tcaggatggg cgccggtggt aatcgactga
    30181 ataatctccc ccgtcgccac gttaatgacc gatactgcat tcgcttcaaa tgaagtcgca
    30241 taaatcaact tttcatccag gcttaatgcc agcgatctga cgccctgatc tttacagcca
    30301 ccacaatgtt tgactgactt ttctttcgct atgggcgctg taaaggtatg caggattttc
    30361 ttttgtccga ggtcgaaaac aacaatgctg ttggtgtttt caattgcggc atacaacgta
    30421 ttaccatccc gactgatcgc catatcatgg atatcggcaa tctcagcgag taaatcacca
    30481 ctggcctgct cacgacttac cggagcggat aatgtttccc tgagggcgtt gcgctcctcc
    30541 actaactgct gctttttttg tcgtcgctct tgttcaatct cctgcggata aaacgcttca
    30601 cgcaggcttt gcctgacctg taaaactgat tcatcaatct gctgctgcac aaaccagata
    30661 gtcccggtca gaagaagagt gaccgcaaca ataatcccgc cttccgtaac gagccgattt
    30721 tgcccaacta aacgcgcact cagccaaccc aacgcgcccc ccgcaatcag gccgccaatg
    30781 tggcaagcgt tatctattcc tgcttgccgg gcaccataca acagtgtcag cgccaccata
    30841 cccaacagag aaaatactcg ccgctggttt ttatggtgtg tacccgcacc tgaagaaagc
    30901 tgtgttgcta tcgcagcgcc cgctattccc ataattgccc cggatgcccc aacgctgatg
    30961 actatctgct cactttcgcg taacgcataa caggcgctga tcagcccacc aaagatgccg
    31021 gagaataacc agataatcag gagtcgccat ttccccagaa tacgttccac cgctcccccc
    31081 accaccagga gtgcaaacat attcatcagc aaatgagaaa cactgctgtg cagaaagaca
    31141 ctgctcaata agcgccagga ttcacccgac aacgtgagag aagcaacgtt agcaccgaga
    31201 tggataagtg cggattgatc gacttcttca taactcatcc attgtgcgag gtaacaaaat
    31261 atcccaacac agagcgcaaa aaggataagg gttattgcgg gtaattgaac atttaacggc
    31321 ttaaccgaag atgcagacat aaggtgattc ttccttgaaa agacaggtaa aaagcatttt
    31381 tcccgtgtgc agttatcatc ccggcaaacg tcccatgccg aaaagataac cctgtccgaa
    31441 atatgactat ttcagcctaa cattacaatc gtaattaatt cacctataag tatttt
//
LOCUS       DAZFLD010000027        31421 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.027, whole genome shotgun sequence.
ACCESSION   DAZFLD010000027 DAZFLD010000000
VERSION     DAZFLD010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31421
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.027"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(115..3264)
                     /gene="acrB"
                     /locus_tag="ACHKBL_001102"
     CDS             complement(115..3264)
                     /gene="acrB"
                     /locus_tag="ACHKBL_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease AcrB"
                     /protein_id="HFO5818003.1"
                     /translation="MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVT
                     ISASYPGADAKTVQDTVTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADI
                     AQVQVQNKLQLAMPLLPQEVQQQGVSVEKSSSSFLMVVGVINTDGTMTQEDISDYVAA
                     NMKDAISRTSGVGDVQLFGSQYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQ
                     LGGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYD
                     IIAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVK
                     ISIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIAVPVVLLGTFAVLAAFGFSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGDHGEG
                     KKGFFGWFNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLPD
                     EDQGVFMTMVQLPAGATQERTQKVLNEVTHYYLTKEKNNVESVFAVNGFGFAGRGQNT
                     GIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDF
                     ELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNGLEDTPQFKIDIDQEKAQALG
                     VSINDINTTLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQM
                     VPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVG
                     YDWTGMSYQERLSGNQAPSLYAISLIVVFLCLAALYESWSIPFSVMLVVPLGVIGALL
                     AATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGLIEATLDAVRMRL
                     RPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVR
                     RRFSRKNEDIEHSHTVDHH"
     gene            complement(3287..4480)
                     /gene="acrA"
                     /locus_tag="ACHKBL_001103"
     CDS             complement(3287..4480)
                     /gene="acrA"
                     /locus_tag="ACHKBL_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrA"
                     /protein_id="HFO5818004.1"
                     /translation="MNKNRGFTPLAVVLMLSGSLALTGCDDKQAQQGGQQMPAVGVVT
                     VKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQ
                     AAYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQEYDQALADAQQANAAVTAA
                     KAAVETARINLAYTKVTSPISGRIGKSNVTEGALVQNGQATTLATVQQLDPIYVDVTQ
                     SSNDFLRLKQELANGTLKQENGKAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITL
                     RAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGDATVLVVGADDKVET
                     RPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQP
                     EQSKS"
     gene            4622..5269
                     /gene="acrR"
                     /locus_tag="ACHKBL_001104"
     CDS             4622..5269
                     /gene="acrR"
                     /locus_tag="ACHKBL_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrR"
                     /protein_id="HFO5818005.1"
                     /translation="MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVT
                     RGAIYWHFKDKSDLFSEIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVT
                     EERRRLLMEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMT
                     RRAAIIMRGYISGLMENWLFAPQSFDLKKEARDYVAILLEMYLLCPTLRNPATNE"
     gene            5397..8759
                     /gene="mscK"
                     /locus_tag="ACHKBL_001105"
     CDS             5397..8759
                     /gene="mscK"
                     /locus_tag="ACHKBL_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HFO5818006.1"
                     /translation="MTMFQYYKRSRHFVFSAFIAFVFVLLCQNTAFARASSNGDLPTK
                     ADLQAQLDSLNKQKDLSAQDKLVQQDLTDTLATLDKIDRVKEETVQLRQKVAEAPEKM
                     RQATAALTALSDVDNDEETRKILSTLSLRQLETRVAQALDDLQNAQNDLASYNSQLVS
                     LQTQPERVQNAMYNASQQLQQIRSRLDGTDVGETALRPSQKVLMQAQQALLNAEIDQQ
                     RKSLEGNTVLQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPD
                     EAARIQANPLVKQELEINQQLSQRLITATENGNQLMQQNIKVKNWLERALQSERNIKE
                     QIAVLKGSLLLSRILYQQQQTLPSADELENMTNRIADLRLEQFEVNQQRDALFQSDAF
                     VNKLEEGHTNEVNSEVHDALLQVVDMRRELLDQLNKQLGNQLMMAINLQINQQQLMSV
                     SKNLKSILTQQIFWVNSNRPMDWDWIKAFPQSLRDEFKSMKITVNWEKAWPAVFIAFL
                     AGLPLLLIAGLIHWRLGWLKAYQQKLASAVGSLRNDSQLNTPKAILIDLIRALPVCLI
                     ILAVGLILLTMQLNISELLWSFSKKLAIFWLVFGLCWKVLEKNGVAVRHFGMPEQQTS
                     HWRRQIVRISLALLPIHFWSVVAELSPLHLMDDVLGQAMIFFNLLLIAFLVWPMCRES
                     WRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLRLSGRWIETVYLVIIWNLLYQ
                     TVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITMLLM
                     FALFGVMFWAIWSDLITVFSYLDSITLWHYNGTEAGAAVVKNVTMGSLLFAIIASMVA
                     WALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMTVFGSLGVSWDKLQW
                     LAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGSFSGTVSKIRIRATTIT
                     DFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRLGVAYGSDLEKVRKVLLKAATEHP
                     RVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCRENDINIAF
                     NQLEVHLHNEKGDEVTEVKRDYKGDDPTPAVG"
     gene            8820..8918
                     /locus_tag="ACHKBL_001106"
                     /pseudo
     CDS             8820..8918
                     /locus_tag="ACHKBL_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(8971..9132)
                     /gene="rsmS"
                     /locus_tag="ACHKBL_001107"
     CDS             complement(8971..9132)
                     /gene="rsmS"
                     /locus_tag="ACHKBL_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pleiotropic regulatory protein RsmS"
                     /protein_id="HFO5818007.1"
                     /translation="MSLENAPDDVKLAVDLIVLLEENQIPARTVLRALDIVKRDYEKK
                     LTRDDEAEK"
     gene            complement(9146..9673)
                     /gene="priC"
                     /locus_tag="ACHKBL_001108"
     CDS             complement(9146..9673)
                     /gene="priC"
                     /locus_tag="ACHKBL_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N''"
                     /protein_id="HFO5818008.1"
                     /translation="MKTALLLEKLEGQLATLRQRCAPVAQFATLSARFDRHLFQTRAT
                     TLQACLDEAGDNLAALRHAVEQQQLPQVAWLAEHLAAQLEAIAREATAWSLREWDSAP
                     PKIARWQRKRIQHQDFERRLREMVAERRARLARATDLVEQQTLHREVEAYEARLARCR
                     HALEKIENRLARLTR"
     gene            9743..10120
                     /gene="ybaN"
                     /locus_tag="ACHKBL_001109"
     CDS             9743..10120
                     /gene="ybaN"
                     /locus_tag="ACHKBL_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HFO5818009.1"
                     /translation="MQRIILIIIGWLAVVLGTLGVVLPVLPTTPFILLAAWCFARSSP
                     RFHAWLLYRSWFGSYLRFWQKHHAMPRGVKPRAILLILLTFAISLWFVQMPWVRIMLL
                     VILACLLFYMWRIPVIDEKQEKH"
     gene            10273..10824
                     /gene="apt"
                     /locus_tag="ACHKBL_001110"
     CDS             10273..10824
                     /gene="apt"
                     /locus_tag="ACHKBL_001110"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HFO5818010.1"
                     /translation="MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALS
                     IDLLVERYKNAGITKVVGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISETYDL
                     EYGTDQLEIHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFD
                     LGGEQRLEKQGITSYSLVPFPGH"
     gene            10953..12884
                     /gene="dnaX"
                     /locus_tag="ACHKBL_001111"
     CDS             10953..12884
                     /gene="dnaX"
                     /locus_tag="ACHKBL_001111"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HFO5818011.1"
                     /translation="MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFS
                     GTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVE
                     DTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQ
                     KLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDAL
                     SLTDQAIASGDGQVSTQAVSAMLGTLDDDQALSLVEAMVEANGERVMALINEAAARGI
                     EWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIELRMRELARTIPPTDIQLYYQTLLI
                     GRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQSFAPVAPTAVMTPTQVPPQ
                     PQSAPQQAPTVPLPETTSQVLAARQQLQRVQGATKAKKSEPAAATRARPVNNAALERL
                     ASVTDRVQARPVPSALEKAPAKKEAYRWKATTPVMQQKEVVATPKALKKALEHEKTPE
                     LAAKLAAEAIERDPWAAQVSQLSLPKLVEQVALNAWKEESDNSVCLHLRSSQRHLNNR
                     GAQQKLAEALSTLKGSTVELTIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNI
                     QTLRRFFDAELDEESIRPI"
     misc_feature    12217..12281
                     /gene="dnaX"
                     /locus_tag="ACHKBL_001111"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00382"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="DnaX frameshifting element; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00382"
     gene            12937..13266
                     /gene="ybaB"
                     /locus_tag="ACHKBL_001112"
     CDS             12937..13266
                     /gene="ybaB"
                     /locus_tag="ACHKBL_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HFO5818012.1"
                     /translation="MFGKGGLGNLMKQAQQMQEKMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRVEIDPSLLEDDKEMLEDLVAAAFNDAARRIEETQKEKMASVSSGMQLP
                     PGFKMPF"
     gene            13266..13871
                     /gene="recR"
                     /locus_tag="ACHKBL_001113"
     CDS             13266..13871
                     /gene="recR"
                     /locus_tag="ACHKBL_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HFO5818013.1"
                     /translation="MQTSPLLTQLMEALRCLPGVGPKSAQRMAFTLLQRDRSGGMRLA
                     QALTRAMSEIGHCADCRTFTEQEVCNICSNPRRQENGQICVVESPADIYAIEQTGQFS
                     GRYFVLMGHLSPLDGIGPDDIGLDRLEQRLAEEKITEVILATNPTVEGEATANYIAEL
                     CAQYDVEASRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF"
     gene            13981..15855
                     /gene="htpG"
                     /locus_tag="ACHKBL_001114"
     CDS             13981..15855
                     /gene="htpG"
                     /locus_tag="ACHKBL_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HFO5818014.1"
                     /translation="MKGQETRGFQSEVKQLLHLMIHSLYSNKEIFLRELISNASDAAD
                     KLRFRALSNPDLYEGDGELRVRVSFDKDKRTLTISDNGVGMTRDEVIDHLGTIAKSGT
                     KSFLESLGSDQAKDSQLIGQFGVGFYSAFIVADKVTVRTRAAGEKPENGVFWESAGEG
                     EYTVADITKEDRGTEITLHLREGEDEFLDDWRVRSIISKYSDHIALPVEIEKREEKDG
                     ETVISWEKINKAQALWTRNKSEITDEEYKEFYKHIAHDFNDPLTWSHNRVEGKQEYTS
                     LLYIPSQAPWDMWNRDHKHGLKLYVQRVFIMDDAEQFMPNYLRFVRGLIDSSDLPLNV
                     SREILQDSTVTRNLRNALTKRVLQMLEKLAKDDAEKYQTFWQQFGLVLKEGPAEDFAN
                     QEAIAKLLRFASTHTDSSAQTVSLEDYVSRMKEGQEKIYYITADSYAAAKSSPHLELL
                     RKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSVSKVDESLEKLADEVDESAKEAEKAL
                     TPFIDRVKALLGERVKDVRLTHRLTDTPAIVSTDADEMSTQMAKLFAAAGQKVPEVKY
                     IFELNPDHVLVKRAADTEDEAKFSEWVELLLDQALLAERGTLEDPNLFIRRMNQLLVS
                     "
     gene            16036..16680
                     /gene="adk"
                     /locus_tag="ACHKBL_001115"
     CDS             16036..16680
                     /gene="adk"
                     /locus_tag="ACHKBL_001115"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004017 - adenylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HFO5818015.1"
                     /translation="MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKSGSE
                     LGKQAKDIMDAGKLVTDELVIALVKERIAQEDCRNGFLLDGFPRTIPQADAMKEAGIN
                     VDYVLEFDVPDELIVDRIVGRRVHAPSGRVYHVKFNPPKVEGKDDVTGEELTTRKDDQ
                     EETVRKRLVEYHQMTAPLIGYYSKEAEAGNTKYAKVDGTKPVAEVRAALEKILG"
     gene            16812..17774
                     /gene="hemH"
                     /locus_tag="ACHKBL_001116"
     CDS             16812..17774
                     /gene="hemH"
                     /locus_tag="ACHKBL_001116"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HFO5818016.1"
                     /translation="MRQTKTGILLANLGTPDAPTPEAVKRYLKQFLSDRRVVDTSRLL
                     WWPLLRGVILPLRSPRVAKLYASVWMEGGSPLMVYSRQQQQALAQRLPETPVALGMSY
                     GSPSLESAVDELLAEHVDHIVVLPLYPQFSCSTVGAVWDELARILARKRSIPGISFIR
                     DYADNHDYINALANSVRASFAKHGEPDLLLLSYHGIPQRYADEGDDYPQRCRTTTREL
                     ASALGMAPEKVMMTFQSRFGREPWLMPYTDETLKMLGEKGVGHIQVMCPGFAADCLET
                     LEEIAEQNREVFLGAGGKKYEYIPALNATPEHIEMMANLVAAYR"
     gene            complement(17771..18730)
                     /gene="aes"
                     /locus_tag="ACHKBL_001117"
     CDS             complement(17771..18730)
                     /gene="aes"
                     /locus_tag="ACHKBL_001117"
                     /EC_number="3.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl esterase"
                     /protein_id="HFO5818017.1"
                     /translation="MKPENKLPVLDLISAEMKTVVNTLQPDLPPWPATGTIAEQRQYY
                     TLERRFWNAGAPEMATRAYMVPTKYGQVETRLFCPQPDSPATLFYLHGGGFILGNLDT
                     HDRIMRLLASYSQCTVIGIDYTLSPEARFPQAIEEIVAACCYFHQQAEDYQINMSRIG
                     FTGDSAGAMLALASALWLRDKQIDCGKVAGVLLWYGLYGLRDSVTRRLLGGVWDGLTQ
                     QDLQMYEEAYLSNDADRESPYYCLFNNDLTREVPPCFIAGAEFDPLLDDSRLLYQTLA
                     AHQQPCEFKLYPGTLHAFLHYSRMMKTADEALRDGAQFFTAQL"
     gene            18882..20186
                     /gene="gsk"
                     /locus_tag="ACHKBL_001118"
     CDS             18882..20186
                     /gene="gsk"
                     /locus_tag="ACHKBL_001118"
                     /EC_number="2.7.1.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/guanosine kinase"
                     /protein_id="HFO5818018.1"
                     /translation="MKFPGKRKSKHYFPVNARDPLLQQFQPENETSAAWVVGIDQTLV
                     DIEAKVDDEFIERYGLSAGHSLVIEDDVAEALYQELKQKNLITHQFAGGTIGNTMHNY
                     SVLADDRSVLLGVMCSNIEIGSYAYRYLCNTSSRTDLNYLQGVDGPIGRCFTLIGESG
                     ERTFAISPGHMNQLRAESIPEDVIAGASALVLTSYLVRCKPGEPMPEATMKAIEYAKK
                     YNVPVVLTLGTKFVIAENPQWWQQFLKDHVSILAMNEDEAEALTGESDPLLASDKALD
                     WVDLVLCTAGPIGLYMAGFTEDEAKRKTQHPLLPGAIAEFNQYEFSRAMRHKDCQNPL
                     RVYSHIAPYMGGPEKIMNTNGAGDGALAALLHDITANSYHRSNVPNSSKHKFTWLTYS
                     SLAQVCKYANRVSYQVLNQHSPRLTRGLPEREDSLEESYWDR"
     gene            complement(20270..21946)
                     /gene="ybaL"
                     /locus_tag="ACHKBL_001119"
     CDS             complement(20270..21946)
                     /gene="ybaL"
                     /locus_tag="ACHKBL_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaL family putative K(+) efflux transporter"
                     /protein_id="HFO5818019.1"
                     /translation="MHHATPLITTIVGGLVLAFILGMLANKLRISPLVGYLLAGVLAG
                     PFTPGFVADTKLAPELAELGVILLMFGVGLHFSLKDLMAVKAIAIPGAIAQIAVATLL
                     GMALSAVLGWSLMTGIVFGLCLSTASTVVLLRALEERQLIDSQRGQIAIGWLIVEDLV
                     MVLTLVLLPAVAGMMEQGDVGFATLAVDMGITIGKVIAFIAIMMLVGRRLVPWIMARS
                     AATGSRELFTLSVLALALGIAFGAVELFDVSFALGAFFAGMVLNESELSHRAAHDTLP
                     LRDAFAVLFFVSVGMLFDPLILIQQPLAVLATLAIILFGKSLAAFFLVRLFGHSQRTA
                     LTIAASLAQIGEFAFILAGLGMALDLLPQAGQNLVLAGAILSIMLNPVLFALLEKYLA
                     KTETLEEQTLEEAIEEEKQIPVDICNHALLVGYGRVGSLLGEKLLASDIPLVVIETSR
                     TRVDELRERGVRAVLGNAANEEIMQLAHLECAKWLILTIPNGYEAGEIVASARAKNPD
                     IEIIARAHYDDEVAYITERGANQVVMGEREIARTMLELLETPPAGEVVTG"
     gene            complement(22184..23404)
                     /gene="fsr"
                     /locus_tag="ACHKBL_001120"
     CDS             complement(22184..23404)
                     /gene="fsr"
                     /locus_tag="ACHKBL_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fosmidomycin MFS transporter"
                     /protein_id="HFO5818020.1"
                     /translation="MAMSEQTQPVAGAAASTTKARTSFGILGAISLSHLLNDMIQSLI
                     LAIYPLLQSEFSLTFMQIGMITLTFQLASSLLQPVVGYWTDKYPMPWSLPIGMCFTLS
                     GLVLLALAGSFGAVLLAAALVGTGSSVFHPESSRVARMASGGRHGLAQSIFQVGGNFG
                     SSLGPLLAAVIIAPYGKGNVAWFVLAALLAIVVLAQISRWYSAQHRMNKGKPKATIIN
                     PLPRNKVVLAVSILLILIFSKYFYMASISSYYTFYLMQKFGLSIQNAQLHLFAFLFAV
                     AAGTVIGGPVGDKIGRKYVIWGSILGVAPFTLILPYASLHWTGVLTVIIGFILASAFS
                     AILVYAQELLPGRIGMVSGLFFGFAFGMGGLGAAVLGLIADHTSIELVYKICAFLPLL
                     GMLTIFLPDNRHKD"
     gene            23622..25274
                     /gene="ushA"
                     /locus_tag="ACHKBL_001121"
     CDS             23622..25274
                     /gene="ushA"
                     /locus_tag="ACHKBL_001121"
                     /EC_number="3.1.3.5"
                     /EC_number="3.6.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HFO5818021.1"
                     /translation="MKLLQRGVALALLTTFTLASEAALAYEQDKTYKITVLHTNDHHG
                     HFWRNEYGEYGLAAQKTLVDGIRKEVAAEGGSVLLLSGGDINTGVPESDLQDAEPDFR
                     GMNLVGYDAMAIGNHEFDNPLTVLRQQEKWAKFPLLSANIYQKSTGERLFKPWALFKR
                     QDLKIAVIGLTTDDTAKIGNPEYFTDIEFRKPADEAKLVIQELQQTEKPDIIIAATHM
                     GHYDNGEHGSNAPGDVEMARALPAGSLAMIVGGHSQAPVCMAAENKKQVDYVPGTPCK
                     PDQQNGIWIVQAHEWGKYVGRADFEFRNGEMKMVNYQLIPVNLKKKVTWEDGKSERVL
                     YTPEIAENQQMISLLSPFQNKGKAQLEVKIGETNGRLEGDRDKVRFVQTNMGRLIMAA
                     QMDRTGADFAVMSGGGIRDSIEAGDISYKNVLKVQPFGNVVVYADMTGKEVIDYLTAV
                     AQMKPDSGAYPQFANVSFVAKDGKLNNLKIKGEPVDPAKTYRMATLNFNATGGDGYPR
                     LDNKAGYVNTGFIDAEVLKAYIQKSSPLDVSVYEPKGEVSWQ"
     gene            complement(25311..25790)
                     /gene="ybaK"
                     /locus_tag="ACHKBL_001122"
     CDS             complement(25311..25790)
                     /gene="ybaK"
                     /locus_tag="ACHKBL_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK"
                     /protein_id="HFO5818022.1"
                     /translation="MTPAVKLLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQV
                     YKTLLVAVNGDMKHLAVAVTPVAGQLDLKKVAKALGAKKVEMADPMVAQRSTGYLVGG
                     ISPLGQKKRLPTIIDAPAQEFATIYVSGGKRGLDIELAAGDLAKILDAKFADIARRD"
     gene            complement(25994..26788)
                     /gene="ybaP"
                     /locus_tag="ACHKBL_001123"
     CDS             complement(25994..26788)
                     /gene="ybaP"
                     /locus_tag="ACHKBL_001123"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HFO5818023.1"
                     /translation="MDLLYRVKTLWAALRGNHYTWPAIDISLPGNRHFHLIGSIHMGS
                     HDMAPLPTRLLKKLKNADALIVEADVSTSDTPFANLPACEALEKRISEEQLQNLQHIS
                     QEMGISPSLFSTQPLWQIAMVLQATQAQKLGLRAEYGIDYQLLQAAKQQHKPVIELEG
                     AENQIAMLLQLPDNGLALLDDTLTHWHTNARLLQQMMSWWLNAPPQNNEITLPNTFSQ
                     SLYDVLMHQRNLAWRDKLCAMPPGRYVVAVGALHLYGEGNLPQMLR"
     gene            26921..27223
                     /locus_tag="ACHKBL_001124"
     CDS             26921..27223
                     /locus_tag="ACHKBL_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000057540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO5818024.1"
                     /translation="MAQKKNIRSFRDAWLADFFVHSTPHRKIPAEIHTTLSRKLDIIN
                     AATSHRDLRSPPGNRYEELSGKLQEYSSIRVNKQYRLIFKWVNGKAEELFLDPHNY"
     gene            27259..27600
                     /gene="ybaQ"
                     /locus_tag="ACHKBL_001125"
     CDS             27259..27600
                     /gene="ybaQ"
                     /locus_tag="ACHKBL_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HFO5818025.1"
                     /translation="MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSAL
                     INNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLWEVENNMRTQEELGRIETVAEYL
                     ARREERAKKVA"
     gene            complement(27658..30162)
                     /gene="copA"
                     /locus_tag="ACHKBL_001126"
     CDS             complement(27658..30162)
                     /gene="copA"
                     /locus_tag="ACHKBL_001126"
                     /EC_number="7.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper-exporting P-type ATPase CopA"
                     /protein_id="HFO5818026.1"
                     /translation="MSQTIDLTLDGLSCGHCVKRVKESLEQRPDVEQADVSVTEAHVT
                     GTASAEQLIETIKQAGYDASVSHPKAKPLAESSIPSEALTAVSEALPAATADDDDSQQ
                     LLLSGMSCASCVTRVQNALQSVPGVTQARVNLAERTALVMGSASPQDLVQAVEKAGYG
                     AEAIEDDAKRRERQQETAVATMKRFRWQAIVALAVGIPVMVWGMIGDNMMVTADNRSL
                     WLVIGLITLAVMVFAGGHFYRSAWKSLLNGAATMDTLVALGTGVAWLYSMSVNLWPQW
                     FPMEARHLYYEASAMIIGLINLGHMLEARARQRSSKALEKLLDLTPPTARLVTNEGEK
                     NVPLADVQPGMLLRLTTGDRVPVDGEITQGEAWLDEAMLTGEPIPQQKGEGDSVHAGT
                     VVQDGSVLFRASAVGSHTTLSRIIRMVRQAQSSKPEIGQLADKISAVFVPVVVVIALV
                     SAAIWYFFGPAPQIVYTLVIATTVLIIACPCALGLATPMSIISGVGRAAEFGVLVRDA
                     DALQRASTLDTVVFDKTGTLTEGKPQVVAVKTFADFDEAQALRLAAALEQGSSHPLAR
                     AILDKASDMQLPQVNGFRTLRGLGVSGEAEGHALLLGNQALLNDQQVDTKAIEADISA
                     QASQGATPVLLAVDGKAVALLAVRDPLRSDSVAALQRLHKAGYRLVMLTGDNPTTANA
                     IAKEAGIDEVIAGVLPDGKAEAIKRLQSEGRQVAMVGDGINDAPALAQADVGIAMGGG
                     SDVAIETAAITLMRHSLMGVADALAISRATLRNMKQNLLGAFIYNSIGIPVAAGILWP
                     FTGTLLNPVVAGAAMALSSITVVSNANRLLRFKPKE"
     gene            30425..31357
                     /gene="glsA"
                     /locus_tag="ACHKBL_001127"
     CDS             30425..31357
                     /gene="glsA"
                     /locus_tag="ACHKBL_001127"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HFO5818027.1"
                     /translation="MLDANKLQQAVDQAYTQFHSLNGGQNADYIPFLANVPGQLAAVA
                     IVTCDGNIYRAGDSDYRFALESISKVCTLALALEDVGPQAVQDKIGADPTGLPFNSVI
                     ALELHGGKPLSPLVNAGAIATTSLINAENTEQRWQRILHIQQQLAGEQVALSDEVNQS
                     EQTTNFHNRAIAWLLYSAGYLYCDAMEACDVYTRQCSTLINTVELATLGATLAAGGVN
                     PLTHKRVLQADNVPYILAEMMMEGLYGRSGDWAYRVGLPGKSGVGGGILAVVPGVMGI
                     AAFSPPLDEEGNSVRGQKMVASVAKQLGYNVFKG"
BASE COUNT         7732 a         8287 c         8227 g         7175 t
ORIGIN      
        1 gtttaatact ggttttcgta tgagatcctg agttggtggt tcaatgactc cttaatgttc
       61 gtcggttatg cataaaaaag gccgcttgcg cggccttagt gattacacgt tgtatcaatg
      121 atgatcgaca gtatggctgt gctcgatatc ttcattcttg cggctaaagc ggcggcgaac
      181 caccacaaag aataccggaa cgaagaagat tgccagtacc gttgcggtca ccatcccgcc
      241 cattacaccg gtacctactg cgttctgcgc gccggaacca gcaccagtac tgataaccag
      301 cggcataacg ccgaggataa acgccaacga ggtcatcagg attgggcgta aacgcatccg
      361 caccgcatca agcgtcgctt caatcagacc tttaccttct ttatccatca agtctttggc
      421 gaattcgacg ataagtatcg cgttcttcgc cgacaaacca atggttgtga gcaggcctac
      481 ctggaagtaa acgtcattgg tcaggccacg gaaggtggca gccagcaacg caccgataac
      541 ccccagcgga acgaccagca taacggagaa cggaatcgac cagctctcgt acagcgcagc
      601 cagacacagg aacacgacaa tcaacgaaat cgcgtacagt gaaggcgcct ggttgccgga
      661 gagacgttcc tgataggaca ttcccgtcca gtcatagcca acaccggtag gcagtttgct
      721 cgccagttgt tccatcagct ccattgcttc accggtactt ttacccggtg ccgcctggcc
      781 taagatttcc atagatggca ggccgttgta acgttccaga cgcggcgaac cgtactccca
      841 acgagaagag gagaacgccg agaacggcac catctgacca tcagcagcac gaacatacca
      901 gtcgccgata tcatccggca gcatacggta tttcgcttct gacatgacgt aaactttctt
      961 cacacgaccg cggtcgataa agtcgttcac atagctaccg ccccatgcag cgcccagagt
     1021 ggtgttaatg tcgttgatag aaacacccag cgcctgcgct ttttcctggt cgatatcaat
     1081 cttaaactgc ggggtatctt ccagaccgtt tggacgtacg ctggtcaaca tatcagggtg
     1141 cttcgctgct tctgcaagca actggttacg cgcctgagtc agtttttcgt gaccaaggcc
     1201 agcctggtca atcagctcaa agtcaaagcc ggttgcggta cccagttcca cgattgcggg
     1261 caggttaaag gcgaaaacca tcgcatcttt gatttgcgag aaagcacgtg ttgcacgcat
     1321 ggtaatcgct tcaactttgt tttcttcgcc cggacgatcg gcccagtcct tcaaggaaac
     1381 gaacgcaata cctgtattct gaccacgtcc cgcaaagccg aagccgttaa cggcgaacac
     1441 cgactcaacg ttgttctttt ctttggtcag atagtaatgc gttacctcat tgagcacttt
     1501 ctgcgtacgt tcctgcgttg cacctgctgg cagctgaacc atggtcataa atacgccctg
     1561 gtcttcatct ggcaagaagg agcttggcag acgcacgaac agataggcca tgccgaccac
     1621 gatgatcaga tacagcacca ggtaacgccc tgtactgcgc agaataccgc ctacgctgtc
     1681 ggtgtagtgg tgcgtgctct tctcgaacat gcggttaaac cagccgaaga agcctttttt
     1741 accttccccg tgatcgcctt tggcaatcgg tttcagcatg gtggcacaaa gagccggagt
     1801 caggatcaac gccaccagta ccgacagcgc cattgctgaa acaatggtaa tagagaactg
     1861 acgatagata gcaccagtag aaccgccaaa gaaggccatc ggtacgaata ccgccgacag
     1921 taccatcgcg ataccgacca gagcgccctg aatctgcccc atcgacttac gggtagcttc
     1981 ttttggcggc aaaccttctt ccgccataac acgctcaacg ttttctacca caacgatggc
     2041 gtcatccacc aacaggccga tggcgagcac catcccgaac attgttagcg tgtttatcga
     2101 gaagccaaag gcggcaagga cggcaaaggt ccccagcaat accaccggta cggcaatggt
     2161 cggaatcaac gtcgcgcgga agttctgcag gaacagatac ataaccagga acacgaggat
     2221 gatcgcttcg actagcgttt taaccacttc gtgaatcgag attttcacga acggcgtggt
     2281 gtcgtacggg taaacaattt tcagacccga cgggaagaac ggttccatct tcgccagttc
     2341 agcacggatt gccgcagcgg tatccagcgc gtttgcaccg gtcgccagct tgatccccag
     2401 accggaagcc ggttggccgt taaactctgc gatgatgtcg tagttctcac cacccagctc
     2461 aattttcgcc acatcacgca gcagcacgcg ggaaccatcc tgattcactt tcagcaggat
     2521 tttgccgaac tcttcagtag aggtcagacg cgtctgagca ataatagagg cgttaagctg
     2581 ttggcctttc accggcggcg taccaccgag ctgacccgcc gcaacctggg cgttctgcgc
     2641 tttgatggcg gtaatgacat caaccggcgt tagctggaat ttgttcagtt cattcgggtt
     2701 catccagata cgcatcgcat actgtgaacc gaacaactga acgtcaccca cgcccgacgt
     2761 acggctgatg gcatctttca tattcgctgc cacgtagtca gagatatcct cctgcgtcat
     2821 ggtgccatcg gtgttgataa cgccgacaac catcaggaag ctgctggatg atttctcaac
     2881 gctcacccct tgctgctgta cttcttgcgg cagcaacggc atcgccagct gcagtttgtt
     2941 ctgcacctga acctgcgcga tatccgcatc agtaccagac tcaaaggtca gggtgatctg
     3001 cacggtaccc gtggagtcac tgttagagga catgtacatc aggttatcga taccgttcat
     3061 attctgttcg ataacctgtg tcaccgtgtc ctgcactgtt ttcgcatcag cgccagggta
     3121 ggaggcggag atcgttactg ccggcggtgc aatcgtagga tattgcgcca ccggcagttt
     3181 gaggatcgcc agcccccctg ccaacatgat gataatggcg atcacccacg caaaaatcgg
     3241 gcgatcgata aagaaattag gcatgtctta acggctcctg tttaagttaa gacttggact
     3301 gttcaggctg agcaccgctt gcggcttgct ggttattatc agcggtaact tcttgtgctt
     3361 ttacctggac accaggacgc actttctgca gcccacttat tactacgcga tcgcctgctt
     3421 tcagaccttc tgtcaccagc cacttatcgc cgatagcctg gcttgcaacg atcggacggg
     3481 tttccacttt gtcatccgcg ccaaccacca gtacggtggc atcgccacgc ggcgtacggg
     3541 ttacgccctg ttgcgggact aaaatagcgt ttggattaag cccttcttcc agacgtgccc
     3601 gcacgaacat acccggcagc agagtgtgat ccgggttcgg gaagatagcg cgtagggtga
     3661 tagacccggt ggtctgatca acggtaacgt cagagaattc cagcgtaccg tcctgcggga
     3721 acttaatgcc gtcactggtg atcagcgaca ctttggcttt gccgttttct tgtttcaacg
     3781 tgccattcgc cagttcctgt ttcaggcgca ggaagtcgtt gctggactgg gtcacatcaa
     3841 cgtagatcgg atcaagttgc tgcacggttg ccagcgtagt cgcctgaccg ttctgtacca
     3901 atgcgccttc cgtcacgttc gacttaccaa tgcgaccgct aatcggcgag gtgactttgg
     3961 tgtaagccag attgatccgc gcagtttcaa cggcagcttt cgccgcagtg accgcagcat
     4021 tcgcctgttg cgcatcagcc agagcctgat cgtactcttg tttactgatg tactgagtac
     4081 cgagcagttt ctgataacga ttcaccgtca attgcgcgat attggctgca gcctgggctt
     4141 tcgccaaatc acctttcgca ctgtcgtatg ccgcctgata ggtcgcagga tcaatctgat
     4201 agagagagac acctgcttcg atgtcgctac cttctttgaa attacgcttc aggataatcc
     4261 cgctaacttg aggacgaact tctgcgatcc ggtaggcact ggtgcgaccc ggaagctcgg
     4321 ttgtgatctg cagaggttca gttttgactg ttactacgcc aacggcgggc atctgctggc
     4381 caccttgttg ggcctgtttg tcgtcacatc ctgttagggc taagctgcct gagagcatca
     4441 gaacgaccgc cagaggcgta aaccctctgt ttttgttcat atgtaaacct cgagtgtccg
     4501 atttcaaatt ggtcaatggt caaaagttaa taaacccatt gctgcgttta tattatcgtc
     4561 gtgctatggt acatacattc acaaatgtat gtaaatctaa cgcctgtaaa ttcacgaaca
     4621 tatggcacga aaaaccaaac aagaagcgca agaaacgcgc caacacatcc tcgatgtggc
     4681 tctacgtctt ttctcacagc agggggtatc atccacctcg ctgggcgaga ttgcaaaagc
     4741 agctggcgtt acgcgcggtg caatctactg gcattttaaa gacaagtcgg atttgttcag
     4801 tgagatctgg gaactgtcag aatccaatat tggtgaactc gagcttgagt atcaggcaaa
     4861 attccctggc gatccactct cagtattaag agagatatta attcatgttc ttgaatccac
     4921 ggtgacagaa gaacggcgtc gattattgat ggagattata ttccacaaat gcgaatttgt
     4981 cggagaaatg gctgttgtgc aacaggcaca acgtaatctc tgtctggaaa gttatgaccg
     5041 tatagaacaa acgttaaaac attgtattga agcgaaaatg ttgcctgcgg atttaatgac
     5101 gcgtcgggca gcaattatta tgcgcggcta tatttccggc ctgatggaaa actggctctt
     5161 tgccccgcaa tcttttgatc ttaaaaaaga agcccgcgat tacgtcgcca tcttactgga
     5221 gatgtatctc ctgtgcccca cgcttcgtaa tcctgccact aacgaataac cttgaatctg
     5281 actccaggat ttttcctgga cattttcgtc gtttctattc tggttcactg cgtcgtgata
     5341 ttcttgcggt ttgacttttt caggtcgttc ttcaggttca gaaaccttca ttcatcatga
     5401 ctatgttcca gtattacaaa cgatcacggc attttgtttt ttcagcattt attgcttttg
     5461 tttttgtctt gttatgccag aacacggcgt ttgcgcgggc gtcatcgaat ggtgatctgc
     5521 cgacaaaagc ggacctgcag gcacaacttg actcactgaa taaacaaaaa gatctttctg
     5581 ctcaggacaa actggtgcag caggatctga cagatacatt agctaccctc gataaaatcg
     5641 atcgcgtaaa agaagagaca gttcagctac ggcaaaaggt tgctgaagcg ccggaaaaaa
     5701 tgcgccaggc gaccgcggcg ttaactgcac ttagcgatgt cgataatgac gaagaaactc
     5761 gcaaaattct gagtacgctt tcgttgcgcc agctggaaac tcgcgttgcc caggcgctgg
     5821 acgatttgca aaacgcacaa aacgatctgg cgtcttataa cagccagctg gtttcgttac
     5881 agacgcagcc cgaacgcgtg caaaatgcga tgtataacgc ttcgcagcag ctgcaacaaa
     5941 ttcgcagtcg tctggatggg actgatgtcg gcgagactgc cttacgtccc agccagaaag
     6001 tgttaatgca ggctcagcag gctttgctga atgccgagat tgaccagcag cgtaaaagcc
     6061 tggaagggaa cacagtctta caggatacct tgcaaaagca acgggattac gtgacggcga
     6121 acagcgcccg tctggagcac cagttgcagc tgttgcaaga agcagtaaac agcaagcgct
     6181 tgactttaac cgaaaaaacg gcgcaggaag ccgtctcccc ggatgaagcc gcgcgtattc
     6241 aggctaatcc gctggtgaag caggaactgg aaattaacca gcagttaagt cagcgtctga
     6301 ttaccgcgac tgaaaacggt aatcagttga tgcagcaaaa cattaaagtc aaaaactggc
     6361 tggagcgggc gctgcaatcg gaacgcaata ttaaagagca gattgccgtc ctgaagggca
     6421 gcctgctgtt gtctcgtatc ctttaccagc aacaacaaac gctgccctcg gcggatgaac
     6481 tggaaaacat gaccaaccgc atcgcggatt tgcgtctcga acagtttgaa gttaaccagc
     6541 agcgtgatgc actcttccag agcgatgcgt tcgtcaacaa actggaagaa ggtcacacca
     6601 acgaagtcaa cagcgaagtt cacgatgcgt tattgcaagt ggttgatatg cgtcgcgaat
     6661 tgctggatca gcttaacaaa cagttgggta accagctgat gatggccatt aacctgcaaa
     6721 tcaaccagca gcagttaatg agtgtgtcga aaaacctgaa atccatcctg actcagcaaa
     6781 tcttttgggt gaatagtaac cgtccaatgg actgggactg gatcaaagcg ttcccgcaaa
     6841 gcctgagaga tgaatttaag tcgatgaaaa tcacggtgaa ctgggaaaaa gcctggcctg
     6901 ccgtttttat cgctttcctc gctggtttgc cgctgctgtt gattgccggg ctgatccact
     6961 ggcgtctggg ctggctgaaa gcgtatcaac aaaaactggc ttccgctgtg ggttccctgc
     7021 gtaacgacag ccagctcaac acaccaaaag cgatccttat cgacctgatc cgtgcgctgc
     7081 cggtgtgcct gattattctc gcggttggcc tgattctgtt gaccatgcag ctcaacatca
     7141 gcgaactgct atggtcgttc agtaaaaaac tggcgatttt ctggctggtg tttggcctgt
     7201 gctggaaagt gttggagaaa aacggcgttg ccgtgcgtca ctttggtatg ccggaacagc
     7261 agaccagcca ctggcgtcgg caaattgtcc gcatcagtct cgcgttgctg cctatccatt
     7321 tctggtctgt ggtggcagaa ctttccccgc tgcatctgat ggatgatgtg ctggggcagg
     7381 cgatgatttt cttcaacttg ctgctgattg ccttcctggt gtggccgatg tgccgcgaaa
     7441 gctggcgtga taaagagtcg cacaccatgc gactggtcac cattaccgta ctgtcgatta
     7501 tcccgattgc gttgatggtg ctgaccgcca cgggttattt ctacaccacg ctgcgcctct
     7561 ccgggcgttg gattgaaacc gtttatttgg tgatcatctg gaacctgctg taccagacgg
     7621 tattgcgcgg cttaagcgta gcggcgcggc gtatcgcctg gcgtcgtgcg ctggcgcgtc
     7681 ggcagaatct ggtgaaagag ggcgcagaag gtgctgaacc gccggaagaa cccaccattg
     7741 cactggagca ggttaaccag cagacgctgc gtattaccat gttgctgatg tttgcgctgt
     7801 tcggtgtcat gttctgggca atttggtccg atttgatcac cgtgttcagc tatctggaca
     7861 gcatcacgct ctggcattac aacggcactg aagctggtgc tgcggtggtg aaaaacgtca
     7921 ccatgggcag tctgttgttt gcgattatcg cctcaatggt ggcctgggcg ttgattcgca
     7981 acctgcctgg tttgctggaa gtgttggtgc tctcgcgcct gaacatgcgc cagggcgcgt
     8041 cgtatgccat tactaccatc cttaactaca tcattattgc cgttggtgcg atgacggtgt
     8101 tcggatcgct gggcgtctcg tgggataaac tccagtggct ggcggcagca ttatccgtag
     8161 gtcttggttt tggtttacaa gagatcttcg gtaacttcgt ttccggtttg atcattctgt
     8221 tcgaacgtcc ggtgcgcata ggcgacacgg taaccattgg tagcttctcg gggacggtaa
     8281 gtaagatccg tattcgtgcg acaacgatta ccgatttcga tcgcaaagaa gtgatcatcc
     8341 cgaacaaagc gtttgttacc gagcgtctga tcaactggtc gttgactgac acgactacgc
     8401 gtctggtgat ccgtctcggc gtagcttatg gctccgatct ggaaaaagtg cgtaaagtgt
     8461 tgctgaaggc ggcgactgag cacccaaggg tgatgcacga accaatgccg gaagtcttct
     8521 ttacggcatt tggtgccagc acgttggatc atgagctgcg tctgtatgtg cgtgaactgc
     8581 gtgaccgtag ccgtactgtc gatgagctga accgtactat cgatcagttg tgccgtgaaa
     8641 acgacatcaa cattgccttt aaccagcttg aagtgcatct gcacaacgag aagggcgatg
     8701 aggtgacgga agtcaaacgc gactacaaag gcgacgatcc gacgccagcg gtagggtaat
     8761 aaccaaaggg ggcgacattt agttgccccg agattactaa caaagtgcgc gttgttcatg
     8821 ccggatgcgg cgtgaccgcc ttatccggcc tacgaaaccg caagaattca atatattgca
     8881 ggagcggtgt aggcctgata agcgtagcgc atcaggcagt tttgcgtttg cccacaatct
     8941 taggggacat ttagcggccc catttatttc tcacttttcc gcctcatcat cgcgcgttaa
     9001 tttcttttca taatcgcgtt ttacaatatc cagcgcgcgc agcacggtgc gggctgggat
     9061 ctgattttct tccagtagca caatcaaatc gacggccagt ttgacatcgt caggggcatt
     9121 ttccagtgac atactctctc cattgctagc gggttaaacg cgctaacctg ttttcgattt
     9181 tttccagcgc atggcggcag cgtgccaggc gcgcttcata ggcttccact tcacgatgca
     9241 gcgtctgctg ttccacgaga tcggtcgctc gtgccagacg ggctctacgt tcggcaacca
     9301 tctcacgtag ccgccgctca aaatcctgat gctgaatacg tttacgctgc cagcgggcaa
     9361 ttttcggtgg tgcgctatcc cactcgcgta gcgaccaggc tgtggcttca cgcgctatag
     9421 cctccagttg cgccgccaga tgttctgcca gccaggcgac ttgcggcagc tgttgctgct
     9481 caaccgcatg acgaagcgca gccagattat cgcccgcctc atcgagacaa gcctgcagtg
     9541 ttgtcgcacg agtctgaaaa agatgcctgt caaaacgggc gcttagcgtg gcaaattgcg
     9601 ctaccggggc acaacgctga cgcagcgtag cgagctgtcc ttccagtttt tccagcagca
     9661 gggcggtttt cacgataaga cctcaatgaa aatgataatt gttatgctaa agtagcaact
     9721 ctgtaagctg acaacactca atatgcaacg aatcatttta atcatcattg gctggctggc
     9781 ggtagtgctg ggtacgctgg gcgtggtatt accggtatta ccgacgacgc cgtttatcct
     9841 gctggcggcc tggtgctttg cccgttcttc cccgcgcttt cacgcctggt tgctgtaccg
     9901 ctcatggttt ggcagctatc tacgtttctg gcagaaacat catgcgatgc cgcgcggcgt
     9961 caaaccgcgg gcgattttgc ttattttgct cacgtttgcc atttctctgt ggttcgtcca
    10021 gatgccatgg gtgcgcatca tgttgctggt aattctcgcc tgtttgcttt tttatatgtg
    10081 gcgaattccg gtgattgatg aaaagcaaga aaagcactga agcacaacaa tcgcagttgc
    10141 aattattgcg tacagccagt acattctggc gttttcgagc acaggcgcag gcggtcaaag
    10201 gttaaacaac tgttactttt gatacgttta aaacgcgccg tgagtaccac cgtaacaagc
    10261 aggcatacac ttatgaccgc gactgcacag cagcttgagt atctcaaaaa tagcatcaaa
    10321 agcattcagg actacccaaa acccggcatt cttttccgtg atgtcaccag cttactggaa
    10381 gacccgaaag cttacgctct cagcatcgac ttgctggttg agcgttacaa aaatgcgggc
    10441 attaccaaag ttgtcggcac cgaagcgcgt ggcttcttgt ttggcgctcc ggtagctctg
    10501 ggtctgggcg ttggctttgt accggtccgt aaaccgggca aactgccgcg tgaaaccatc
    10561 agtgaaactt acgacctgga atacggcacc gatcagctgg aaatccacgt tgatgccatc
    10621 aaaccgggcg acaaagttct ggtggtggac gatctgctgg caaccggcgg cactatcgaa
    10681 gcgaccgtta aactgatccg tcgtctgggt ggtgaagtgg ctgacgctgc gttcattatc
    10741 aacctgttcg atctcggcgg cgaacagcgt ctcgaaaaac agggcattac cagctacagc
    10801 cttgtcccgt tcccgggcca ttaattatcg ccagtcttgt gctgcccacg ctacggacag
    10861 cacaagatgt gcattcagcc tcgccgttct gacggggctg tgttagcatt accccttcgt
    10921 gaatccacct tccagcgttt cagagcctgc caatgagtta tcaggtctta gcccgaaaat
    10981 ggcgcccaca aacctttgct gacgtcgtcg gccaggaaca tgtgctgacc gcactggcga
    11041 acggcttgtc gttagggcgt attcatcatg cttatctttt ttccggtacc cgtggcgtcg
    11101 gaaaaacctc tatcgcccga ctgctggcga aggggctaaa ctgcgaaacc ggcattaccg
    11161 cgacgccgtg cggcgtgtgc gataactgtc gtgaaatcga gcaggggcgc tttgtcgatc
    11221 tgattgaaat cgacgccgcc tcgcgcacta aagtcgaaga tacccgcgac ctgctggata
    11281 acgttcagta cgctccggcg cgtggtcgtt tcaaagttta tctcatcgac gaagtgcata
    11341 tgctgtcgcg tcacagcttt aacgcactgt taaaaaccct tgaagagccg ccagagcacg
    11401 ttaagttttt gctggcgacg accgatccgc agaaattgcc ggtgacgatt ttgtcacgct
    11461 gtctgcaatt tcatctcaag gcgctggatg tcgagcaaat tcgccatcag cttgagcaca
    11521 tcctcaacga agaacatatc gctcacgagc cgcgggcgct gcaattgttg gcacgcgccg
    11581 ctgaaggcag cctgcgagat gctttaagtc tgaccgacca ggcgattgcc agcggtgacg
    11641 gccaggtttc aacccaggcg gtcagtgcga tgctgggtac gcttgacgac gatcaggcgt
    11701 tatcgctggt tgaagcgatg gtcgaggcca acggcgagcg cgtaatggcg ctaattaatg
    11761 aagctgctgc ccgtggtatc gagtgggaag cgttgctggt ggaaatgctc ggtctgttgc
    11821 atcgtattgc gatggtacaa ctttcgcctg ctgctcttgg caacgacatg gccgccattg
    11881 agctgcggat gcgtgaactg gcgcgcacca taccgccgac ggatattcag ctttactatc
    11941 agacgctgtt gattggtcgc aaagaattac cgtatgcgcc agaccgccgt atgggtgttg
    12001 agatgacgct gctgcgcgcg ctggcgttcc atccgcgtat gcctctgcct gagccagaag
    12061 tgccacgcca gtcctttgcg cccgttgcgc caacggcagt aatgacgcca acacaggtgc
    12121 cgccgcaacc gcaatcagcg ccgcagcagg caccgactgt accgctcccg gaaaccacca
    12181 gccaggtgct ggcggcgcgc cagcagttgc agcgcgtgca gggagcaacc aaagcaaaaa
    12241 agagtgaacc ggcagccgct acccgcgcgc ggccggtgaa taacgctgcg ctggaaagac
    12301 tggcttcggt caccgatcgc gttcaggcgc gcccggtgcc atcggcgctg gaaaaagcgc
    12361 cagccaaaaa agaagcgtat cgctggaagg cgaccactcc agtgatgcag caaaaagaag
    12421 tggtcgccac gccgaaggcg ctgaaaaaag cgctggaaca tgaaaaaacg ccggaactgg
    12481 cggcgaagct ggcggcagaa gccattgagc gcgacccgtg ggcggctcag gtgagtcaac
    12541 tttcgctacc aaaactggtc gaacaggtgg cgttaaatgc ctggaaagag gagagcgaca
    12601 actcagtatg tctgcatttg cgctcctctc agcggcattt gaacaaccgc ggcgcacagc
    12661 aaaaactggc tgaagcgttg agcacgttaa aaggttcaac ggttgaactg actatcgttg
    12721 aagatgataa tcccgcggtg cgtacgccgc tggaatggcg tcaggcgata tacgaagaaa
    12781 aacttgcgca ggcgcgcgag tccattattg cggataataa tattcagacc ctgcgtcgat
    12841 tcttcgatgc ggagctggat gaagaaagta tccgccccat ttgatcgtaa gcacagctta
    12901 cgttcgtcat ccttaacgtg attgagagag aaacctatgt ttggtaaagg cggtctgggt
    12961 aacctgatga agcaagccca gcagatgcaa gaaaaaatgc agaaaatgca ggaagagatc
    13021 gcgcagctgg aagtcaccgg cgaatctggc gcaggtctgg taaaagtgac catcaacggt
    13081 gcacacaact gccgtcgcgt agagatcgac ccgagcctgc tggaagacga caaagagatg
    13141 ctggaagacc tggtggctgc agcattcaac gacgcagcac gtcgtattga agaaacgcag
    13201 aaagaaaaaa tggcctctgt atcctccgga atgcagctgc cgcctggctt taagatgccg
    13261 ttctgatgca aaccagcccg ctgttaacac agcttatgga agcactgcgc tgtctgccgg
    13321 gcgttggccc gaagtcggcg cagcgtatgg cgttcacgct gcttcagcgc gatcgtagcg
    13381 gcgggatgcg tctggcgcag gcgctcaccc gggcgatgtc ggaaatcggc cactgcgccg
    13441 attgccgcac cttcaccgaa caggaagtct gtaacatctg ttcgaatcca cgtcgtcagg
    13501 aaaacggtca aatctgcgtg gtggagagtc cggcggacat ttacgccatt gagcagacgg
    13561 ggcagttttc aggtcgttat tttgtgttga tgggacacct gtcaccgctg gacggcatcg
    13621 gtcctgatga tatcgggctt gatcgtctgg aacagcgtct ggcagaggaa aaaatcactg
    13681 aagtgatcct cgccaccaac cccacggttg aaggtgaagc taccgctaac tacattgccg
    13741 agctttgtgc gcaatatgac gtggaagcca gccgaatcgc tcatggcgta ccggttggcg
    13801 gcgaactgga aatggtcgat ggcaccacgc tgtcacattc ccttgccggg cgtcataaga
    13861 ttcgttttta agcaaacgag agcaggatca cctgctctcg cttgaaatta ttctcccttg
    13921 tccccatctc tccctcatcc tgtttttaac cttaaaatgg cattattgag gtagacctac
    13981 atgaaaggac aagaaactcg tggttttcag tcagaagtga aacagcttct gcacctgatg
    14041 atccattctc tctattccaa taaagaaatc ttcctgcgtg agcttatctc taacgcctcc
    14101 gatgcggcgg acaagctgcg tttccgcgcg ctttctaacc cggacctgta cgaaggtgat
    14161 ggtgaactac gcgttcgtgt ctctttcgat aaagacaagc gcacgctgac catctccgat
    14221 aacggcgtgg ggatgacccg cgacgaagtg attgaccatc tggggactat cgctaaatcc
    14281 ggcaccaaat cattcctcga atccctgggt tctgaccagg cgaaagacag ccagctgatc
    14341 ggtcagtttg gtgttggttt ctactctgcg tttatcgtgg ctgacaaagt gaccgtgcgt
    14401 actcgcgcag caggcgaaaa accagaaaat ggcgtcttct gggaatcggc tggtgaaggt
    14461 gaatacactg ttgccgacat caccaaagaa gatcgtggta ctgaaatcac cctgcatctg
    14521 cgtgaaggcg aagacgagtt cctcgatgac tggcgtgtgc gttccatcat cagcaaatat
    14581 tccgaccata tcgcgctgcc ggtagagatc gaaaaacgcg aagagaaaga cggcgaaacc
    14641 gttatctcct gggagaaaat caacaaagcg caggcgctgt ggactcgtaa caagtcggaa
    14701 atcaccgatg aagagtacaa agagttctac aaacacatcg cccacgactt taacgatccg
    14761 ctgacctgga gccacaaccg cgttgaaggt aagcaggagt acaccagcct gctgtacatt
    14821 ccgtctcagg caccgtggga tatgtggaac cgcgatcata aacacggtct gaaactgtat
    14881 gttcagcgtg tgttcatcat ggacgacgca gaacagttca tgccgaacta tctgcgcttc
    14941 gtgcgtggtc tgattgactc cagcgatctg ccgctgaacg tttcccgtga aatcctccag
    15001 gacagcacgg taacgcgtaa cctgcgcaat gcgctgacca agcgtgtgct gcaaatgctg
    15061 gaaaaactgg cgaaagacga cgcggaaaaa taccagacct tttggcaaca gtttggcctg
    15121 gtactgaaag aaggtccggc ggaagatttc gctaaccagg aagcgatcgc caaactgctg
    15181 cggtttgcgt ctacccatac cgattcttct gcgcagaccg tgtctctgga agactacgtt
    15241 tcccgcatga aagaagggca ggagaaaatc tactacatca ctgcagacag ctacgcggca
    15301 gcgaagagca gcccgcacct ggaactgctg cgtaagaaag gcatcgaagt tctgctgctt
    15361 tccgaccgca tcgatgagtg gatgatgaac tatctgactg agttcgacgg taaaccgttc
    15421 cagtctgtgt ctaaagttga cgagtcgctg gaaaaactgg ctgacgaagt tgatgagagc
    15481 gcgaaagaag cggagaaagc actgactccg tttatcgacc gtgtgaaagc cctgctcggc
    15541 gagcgcgtga aagatgtccg tctgactcac cgtctgaccg atacgccagc gatcgtttcg
    15601 accgacgcgg acgaaatgag cactcagatg gcgaaactgt tcgctgcggc gggccagaaa
    15661 gtgccagaag tgaaatacat cttcgaactg aacccggatc acgtactggt gaaacgtgcg
    15721 gcagatactg aagatgaagc caagttcagc gagtgggtag aactgctgct ggatcaggcg
    15781 ctgctggcag aacgcggcac gctggaagat ccgaacctgt ttattcgtcg tatgaaccag
    15841 ctgctggttt cctgatgtca tgccggatga cctttgtgtc atccggcatt tttcttttca
    15901 tcatctgcac tttccgcaaa ttatctcgcc attaaccgtt tcagccccag gtgcctttct
    15961 tgaggcaatc gcctgttggt ggtatcgttt atcgcttttt caaaaaattc gacacatttt
    16021 aaggggattt tcgcaatgcg tatcattctg cttggcgctc cgggcgcggg gaaagggact
    16081 caggctcagt tcatcatgga gaaatatggt attccgcaaa tctccactgg cgatatgctg
    16141 cgtgctgcgg tcaaatctgg ctccgagctg ggtaaacaag caaaagacat tatggatgct
    16201 ggcaaactgg tcaccgacga actggtgatc gcgctggtta aagagcgcat tgctcaggaa
    16261 gactgccgca acggtttcct gttggacggc ttcccgcgta ccattccgca ggcagacgcg
    16321 atgaaagaag cgggcatcaa tgttgattac gttctggaat tcgacgtacc ggacgaactg
    16381 attgttgacc gtatcgtagg ccgccgcgtt cacgcgccgt ctggtcgtgt ttatcacgtt
    16441 aaattcaatc cgccgaaagt agaaggcaaa gacgacgtta ccggtgaaga gctgactacc
    16501 cgtaaagatg atcaggaaga gaccgtacgt aaacgtctgg ttgaatacca tcagatgact
    16561 gcaccgctga tcggctacta ctctaaagaa gcggaagcgg gtaacaccaa atacgcgaaa
    16621 gttgacggca ccaagccggt ggctgaagtt cgcgcagctc tggaaaaaat tctcggctaa
    16681 tcaagccgca gcagatctgc tttcgggcag gtctgctcat cctcctcagg caatacctct
    16741 cacagcaatt agttcttctt cttcactttt ccgctacaat tatcaacaag ttgaatcgat
    16801 aagaggcggt aatgcgtcag acaaaaaccg gtatcctgct ggcaaacctg ggtacgcccg
    16861 atgcccccac acctgaagcg gtaaaacgct atctgaaaca atttttaagc gacagacgtg
    16921 tggttgatac ctcacggttg ttatggtggc cattgctgcg cggcgtgatt ttgccactgc
    16981 gctcgccgcg tgtggcgaag ctgtatgcct ctgtctggat ggaaggtggc tcgccgctga
    17041 tggtttacag ccgccagcaa cagcaggcgc tggcacaacg tttaccggag acgcccgtag
    17101 cgctgggaat gagctacggc tcgccatcac tggaaagcgc cgtagatgaa ctactggcag
    17161 agcatgtaga tcatattgtg gtgctgccgc tttatccgca attctcctgt tcaacggtcg
    17221 gtgcggtatg ggatgaactg gcacgcattc tggcgcgcaa acgtagcatt ccggggatat
    17281 cgtttattcg tgattacgcc gataaccacg attatattaa tgcactggcg aacagcgtac
    17341 gtgcttcttt tgccaaacat ggcgaaccgg atctgctgtt gctctcttat catggcattc
    17401 cccagcgtta tgcagatgaa ggcgatgatt acccgcaacg ttgtcgcaca acgactcgcg
    17461 aactggcttc cgcactgggg atggcaccgg aaaaagtgat gatgaccttt cagtcgcgct
    17521 ttgggcggga accctggctg atgccttata ccgacgaaac gctgaaaatg ctcggagaaa
    17581 aaggtgtagg tcatattcag gtgatgtgcc cgggctttgc tgcggattgt ctggagacgc
    17641 tggaagagat tgccgagcaa aaccgtgagg tcttcctcgg tgcgggcggg aaaaaatacg
    17701 aatatattcc ggcacttaat gccacgccgg aacatattga aatgatggct aatcttgttg
    17761 ccgcgtatcg ctaaagctga gcggtaaaga actgagcgcc gtcgcgaaga gcctcgtcgg
    17821 cggttttcat catccgtgaa taatgcaaaa aggcgtgcag cgtgcctggg tagagtttga
    17881 actcacaggg ctgctgatgc gccgctaacg tctggtaaag cagacggctg tcatccagca
    17941 gcggatcgaa ctccgccccg gcaataaaac agggcggaac ttcgcgagtg agatcattat
    18001 taaacaggca gtaatacggc gactcgcggt ccgcgtcgtt gcttaaatac gcctcttcat
    18061 acatctgtaa atcctgttgc gttaagccat cccagacacc gcccaacaga cgacgagtca
    18121 cggaatcccg taatccgtaa agcccatacc acagcaaaac gcccgcaact ttaccgcaat
    18181 cgatctgttt atcacgcaac cacaacgcac tggcgagcgc caacatggca cctgcggaat
    18241 caccggtaaa gccaatgcgg gacatattga tttgataatc ttccgcctgc tggtggaaat
    18301 aacaacaggc agccacaatt tcctctattg cttgcggaaa acgcgcttca ggtgaaaggg
    18361 tgtaatcaat gccaatcacc gtacattggc tgtagcttgc cagcaggcgc atgatgcgat
    18421 cgtgggtatc aagattgccg agaataaaac cgccgccatg caaataaaat agcgtcgccg
    18481 ggctatctgg ctgcggacaa aagagacgtg tttccacctg cccatatttt gttggaacca
    18541 tgtaagctct ggttgccatt tctggagcgc ccgcattcca gaatcggcgc tcaagcgtgt
    18601 aatactgtcg ttgctcagca atcgttcccg ttgcgggcca gggcggtaaa tccggctgaa
    18661 gagtattcac aacggtcttc atttcagcag aaataaggtc cagaacgggt aatttgtttt
    18721 ccggcttcat gcaaaaactc ctttcaaatt acgtcattgt aaggaaccac tgccatgaaa
    18781 atgcgatccc gcctgctgat attgaaactg gctgcgtctc gcgcgctccc gtcagattgt
    18841 gttaacattc gccgctcagt taaccacccg taaaaacaac catgaaattt cccggcaaac
    18901 gtaaatccaa acattatttt cccgtaaacg cacgcgatcc gctgcttcag caattccagc
    18961 cagaaaacga aaccagcgct gcctgggtag tgggtatcga tcaaacgctg gtcgatattg
    19021 aagcgaaagt ggatgatgaa tttattgagc gttatggatt aagcgccggg cattcactgg
    19081 tgattgagga tgacgtagcc gaagcgcttt atcaggaact aaaacagaaa aacctgatta
    19141 cccatcagtt tgcgggtggc accattggta acaccatgca caactactcg gtgctcgcgg
    19201 acgaccgttc ggtgctgctg ggcgtcatgt gcagcaatat tgaaattggc agctatgcct
    19261 atcgttacct gtgtaacact tccagccgta ccgatcttaa ctatctacaa ggcgtggatg
    19321 gcccgattgg tcgttgcttt acgctgattg gcgagtccgg ggaacgtacc tttgctatca
    19381 gccccggcca catgaaccag ctgcgggctg aaagtattcc ggaagatgtg attgccggag
    19441 cctcggctct ggttctcacc tcttatctgg tgcgttgcaa gccgggtgaa cccatgccgg
    19501 aagcaaccat gaaagctatt gagtacgcga agaaatataa cgtaccggtg gtgctgacgc
    19561 tgggcaccaa gtttgtcatt gccgagaatc cgcagtggtg gcagcaattc ctcaaagacc
    19621 acgtctctat ccttgcgatg aacgaagatg aagccgaagc gttgaccgga gaaagcgatc
    19681 cgttgttggc atctgacaag gcgctggact gggttgatct ggtgctgtgt accgcagggc
    19741 caatcggctt gtatatggcg ggctttaccg aagacgaagc gaaacgtaaa acccagcatc
    19801 cgctgctgcc gggtgctata gcggaattta accagtatga gtttagccgc gccatgcgcc
    19861 acaaggattg ccagaatccg ctgcgtgtct attcgcacat tgcgccgtac atgggcgggc
    19921 cggaaaaaat catgaacact aatggagcag gggatggcgc actggcagcg ttgctgcatg
    19981 acattaccgc caacagctac caccgtagca acgtaccaaa ctccagcaaa cataaattca
    20041 cctggttaac ttattcatcg ttagcgcagg tgtgtaaata tgctaaccgt gtgagctatc
    20101 aggtactgaa ccagcattca cctcgtttaa cgcgcggctt gccggagcgt gaagacagcc
    20161 tggaagagtc ttactgggat cgttaagtta tcgaccagac cggacgacga tggcatccgt
    20221 aggccggata agatgcgtca gcatcgcatc cggcaatgga atcaatgttt taccccgtca
    20281 ccacctcacc cgccggtggc gtttccagca gttccagcat agtacgagca atttcacgct
    20341 cacccatcac tacctgattc gcaccacgtt cggtgatata cgccacttca tcgtcataat
    20401 gggcgcgggc aataatctca atatccggat ttttcgcgcg ggcagatgcc acaatctcgc
    20461 ccgcttcata accgttggga atcgtcagga tcagccattt tgcacattcc agatgcgcca
    20521 gttgcataat ttcttcgttc gccgcattgc ccaatactgc gcggaccccg cgctctcgca
    20581 gttcatcaac acgggttcgt gacgtctcaa tcaccaccag cggaatatca gaggcgagca
    20641 atttctcccc cagcaggctg cctacacgac cgtaacctac cagtagcgca tggttgcaaa
    20701 tatccactgg gatctgcttc tcttcttcga tagcctcttc cagcgtctgc tcttccagcg
    20761 tttcggtctt cgccagatat ttctccagca gcgcaaacag gaccgggttg agcataatcg
    20821 acaaaatcgc ccctgccagt accaggttct gcccggcttg cggcaataga tccagcgcca
    20881 tgcccagtcc cgccaggata aacgcgaact caccaatttg cgccaggctg gcggcgatag
    20941 ttaatgccgt acgttgggag tgaccaaaca gtcgcaccag gaaaaacgcg gctaacgact
    21001 taccaaacag aataatcgcc agcgtcgcca gcactgccag cggttgctga atcagaatta
    21061 acggatcaaa caacatcccg acggagacaa aaaacagcac cgcaaacgcg tcgcgcaatg
    21121 gcagcgtatc gtgggcggca cggtgactca gttcagactc gttcagtacc atcccggcaa
    21181 agaacgcacc gagtgcaaag gagacatcaa acagctccac cgcaccaaag gcaatcccta
    21241 acgccagcgc cagcaccgac agggtaaaca gctcacggga accggttgct gcgctacgtg
    21301 ccataatcca cggcaccaga cggcgaccta ccagcatcat aatggcgata aatgcgatca
    21361 ccttgccgat ggtgatcccc atatcgacag caagagtggc aaagcccaca tcgccttgtt
    21421 ccatcattcc tgccactgcg ggcagcaaca ccagcgtcag aaccatgacc aggtcttcca
    21481 caatcaacca accgatggcg atttgcccac gctgactgtc aattaattgc cgttcttcaa
    21541 gtgcgcgcag taacaccacg gtactggcgg tggaaaggca taagccgaac acgataccgg
    21601 tcattaacga ccagcccagc acggcggaga gcgccatacc cagcagcgtc gccacggcta
    21661 tctgggcgat cgcaccggga atggcgatgg cctttaccgc catcaaatcc ttcagcgaaa
    21721 agtgcagacc cacgccaaac atcaacagaa tgacgcccag ttcagccagt tccggcgcaa
    21781 gcttggtatc ggcaacaaag cccggagtga atggtcctgc cagcacaccc gctaacagat
    21841 atcccaccag aggagaaata cgtagtttat tggccagcat gccgaggata aaggcgagca
    21901 caaggccgcc aacaatggtg gtgataagcg gggtggcgtg atgcattccg tctccttttc
    21961 ctggtggtta ttgtccattt ttggccggga aaaccaaaat tacaggtaat agtttatgac
    22021 aatttcattg atgatgttta tgaataattg ttgaattttg cagaaaaatg gaattagctg
    22081 caaaaaaagc acggatcgga aaacagaagg cggtttaaca gaggaaaggc ttatggcgac
    22141 tgtaaaacga tgcagccaaa gtttgccttt ggctgcaatg aaatcagtct ttatgccggt
    22201 tatcaggcag gaatatggtc aacatcccca atagtggcag gaaagcacag attttataga
    22261 ctaactcgat gctggtgtga tcggcgataa gccccagaac tgccgctccc agacctccca
    22321 tgccaaaagc aaaaccgaaa aagagtccag aaaccatacc gatacgtccc ggaagtagct
    22381 cctgagcgta gaccagaatg gcagagaatg ccgaagcgag gataaatcca ataatcaccg
    22441 ttaaaacccc cgtccagtgc aggctggcgt agggtaaaat cagcgtaaac ggcgcaacgc
    22501 cgaggataga gccccaaatc acatatttcc gtccaatttt atcccctaca ggcccgccaa
    22561 tcaccgtacc tgccgcaacg gcaaacagga aggcaaacaa atgaagctga gcattctgga
    22621 tagataatcc gaatttttgc atcagataaa aggtgtaata gctgctgatg ctcgccatat
    22681 agaaatattt cgagaaaatg aggattaaca gaatgctgac cgccagaaca actttattgc
    22741 gcggcagtgg attgataatc gtcgctttgg gttttccttt attcattcgg tgctgtgccg
    22801 agtaccaacg gctgatttgc gccaacacca cgatcgccag cagtgccgca agcacaaacc
    22861 aggcaacgtt gcctttgcca taaggcgcga taatcaccgc cgccagcaag gggcccaggg
    22921 aactgccaaa gttgccgccg acctgaaaga tagattgcgc caggccatga cgcccgccgg
    22981 aagccatacg ggccacgcga gaagattccg gatgaaagac cgatgaaccg gtaccgacca
    23041 gcgccgctgc cagcagaact gcgccgaaac tgcccgccag cgccagcagc accagaccac
    23101 ttaaggtaaa gcacatgcca attggcaacg accacggcat cggatattta tcggtccagt
    23161 agccgacaac tggttgcagt agcgaagagg cgagctggaa ggtgagggtt atcatgccaa
    23221 tctgcataaa tgtcagagaa aactctgact gaagcagcgg ataaatcgcc agaatcagcg
    23281 attggatcat gtcgttcagc agatgtgaga ggctgatagc acctaaaata ccaaacgatg
    23341 ttcgggcctt ggtcgttgac gcagccgcgc ccgccacagg ctgggtttgt tcactcattg
    23401 ccataggaaa gtcacttttt cagggttgcg atgtaaagaa tgatcttatt tgtgattatt
    23461 acccgactaa catacctgta tgcgccgcct gaaggaagtc tcaacgctga atacagaatt
    23521 tctaatctgg atacagattt atcttcaccg gacgcagact tgtctatgat gtcgcgtcat
    23581 actatttttc aacacgttga aatcaggtca gggagagaag tatgaaatta ttgcagcggg
    23641 gcgtggcgtt agcactgtta accacattta cactggcgag tgaagctgct ctggcgtatg
    23701 agcaggataa aacctacaaa attacagttc tgcataccaa tgatcatcat gggcattttt
    23761 ggcgcaatga atatggcgaa tatggtctgg cggcgcaaaa aacgctggtg gatggtatcc
    23821 gcaaagaggt tgcggctgaa ggcggtagcg tgctgctact ttccggtggc gacattaaca
    23881 ctggcgtgcc cgagtccgac ttacaggatg ccgaacctga ttttcgcggt atgaatctgg
    23941 tgggctatga cgcgatggcg atcggtaatc atgaatttga taacccgctc accgtattac
    24001 gccagcagga aaagtgggct aagttcccgt tgctttccgc caatatctac cagaaaagta
    24061 ccggcgagcg cctgtttaaa ccatgggcgc tgtttaagcg tcaggatctg aaaattgccg
    24121 ttattggcct gacgacggat gacacagcca aaattggtaa tccggaatat ttcactgata
    24181 tcgaatttcg taagcccgct gatgaagcga agctggtgat tcaggagctg caacagacag
    24241 aaaagccaga cattattatc gcggcgaccc atatggggca ttacgacaat ggtgagcacg
    24301 gctctaacgc accgggcgat gtggagatgg cacgcgcgct gcctgccgga tcgctggcga
    24361 tgatcgtcgg tggccactcg caagctccgg tctgcatggc ggcagaaaac aaaaaacagg
    24421 tcgattacgt gccgggtacg ccatgcaaac cggatcaaca aaacggcatc tggattgtgc
    24481 aggcgcatga gtggggcaaa tacgtgggac gggctgattt tgagtttcgt aatggcgaaa
    24541 tgaaaatggt taactaccag ctgattccgg tgaacctgaa gaagaaagtg acctgggaag
    24601 acgggaaaag cgagcgcgtg ctgtacaccc ctgaaatcgc tgaaaaccag caaatgatct
    24661 cgctgttatc accgttccag aacaaaggca aagcgcagct ggaagtgaaa ataggcgaaa
    24721 ccaatggtcg tctggaaggc gatcgtgaca aagtacgttt tgtacagacc aatatggggc
    24781 ggttgattat ggcagcacaa atggatcgca ctggtgccga ctttgcggtg atgagcggag
    24841 gcggaattcg tgattctatc gaagcaggtg atatcagcta taaaaacgta ctgaaagtgc
    24901 agccattcgg caatgtggtg gtgtatgccg acatgaccgg taaagaggtg atcgattatt
    24961 tgaccgccgt cgcgcagatg aagccagatt caggtgccta cccgcaattt gccaacgtta
    25021 gctttgtggc gaaagacggt aaactgaaca acctgaaaat caaaggcgaa ccggtcgatc
    25081 cggcgaaaac ttaccgcatg gcgacattaa acttcaatgc caccggcggt gatggctatc
    25141 cgcgccttga taacaaagcg ggctatgtga ataccggctt tattgatgcc gaagtgctga
    25201 aagcgtatat ccagaaaagc tcgccgctgg atgtgagtgt ttatgaaccg aaaggtgagg
    25261 tgagctggca gtaatccgaa agtgccggat gttcgcatcc ggcacaatgc ttaatcgcga
    25321 cgggcgatat cagcaaattt ggcatcgagg atctttgcca gatcgcctgc cgccagttcg
    25381 atatccagcc cgcgcttgcc gccggaaaca taaatagtgg caaattcttg tgcgggggcg
    25441 tcgataatcg ttggcagacg ttttttctgc cccagtgggc taatcccccc taccagatac
    25501 cccgtcgaac gctgcgcgac catcggatcg gccatctcaa ctttcttggc acccagcgct
    25561 tttgctactt ttttaagatc cagttgacct gcgaccggcg taacggccac ggcaaggtgt
    25621 ttcatatcac cgttcactgc gaccagcagc gttttgtaga cctgatccgg attcaaacct
    25681 aattttttga cgacttcatc gccaaaattg gtttcagccg gatcgtgctc gtaggtgtgg
    25741 atttgaaacg aaatcttgtt tttttcgagt aatttaactg cgggtgtcat ggtaattttc
    25801 ctgccttaaa caaaaaaacg aagcaagcat acgcctgatg gcggtccaaa aaaaagagtc
    25861 atcttgccta agagtattgg caggatggtg agattgagcg acaatcgagt tacaccgtcg
    25921 cttaaagtga cggcataata ataaaaaaat gaaattcctc tttgacgggc caatagcgat
    25981 attggccatt tttttagcgc aacatctgcg gcaaattccc ttctccatac aggtgtagtg
    26041 caccaaccgc gaccacatag cgccccggcg gcatggcgca taatttgtcc cgccaggcga
    26101 gatttcgctg atgcatcagc acatcgtaca gcgactgact gaacgtatta ggcagcgtta
    26161 tttcattatt ttgcggcggc gcattcagcc accagctcat catttgttgc agcaaccgtg
    26221 cgttggtgtg ccagtgggtc agcgtatcgt ccagcagcgc cagtccgttg tcagggagct
    26281 ggagcaacat ggcaatctgg ttttcagccc cttccagttc aatcacgggt ttatgttgtt
    26341 gcttagccgc ctgcaatagc tggtaatcga taccgtattc tgcccgcagc cccagttttt
    26401 gcgcctgcgt cgcctgaaga accatcgcga tttgccacag cggttgagta gaaaagagcg
    26461 acggagaaat gcccatctcc tggctaatat gctgcaggtt ttggagttgc tcctcgctaa
    26521 tgcgcttttc cagggcctcg caggcaggca aattggcaaa aggcgtatcg ctggtggaaa
    26581 catccgcctc gacgatcagc gcatcggcgt ttttgagctt tttgagcaaa cgggtgggca
    26641 ggggagccat atcgtggcta cccatatgaa tactgccgat cagatgaaaa tggcgattgc
    26701 cgggaagaga gatatcgata gcaggccagg tgtaatgatt accgcgcagc gcggcccaaa
    26761 gcgtttttac ccggtacaac agatccatac gacctccctt tgtgaaatat catgctagcg
    26821 cgcggggagg gatggcgcaa atggagttga ttgttgcact tgcgcacagt gcttgtgtgc
    26881 attacaatga ttaaataatg gacaaggaga tgagagagga atggctcaaa agaaaaatat
    26941 aaggagtttt cgtgatgcct ggttggctga tttttttgtc cattcaacgc cacatcgaaa
    27001 gatccctgct gaaatccata ccacgttatc acgaaagttg gacatcatta atgctgccac
    27061 ttctcatcgg gatttacgct cccctccggg gaaccgatac gaagagttat ccgggaaatt
    27121 acaggaatac tcctccatta gagtgaataa acagtaccga ctgattttta aatgggttaa
    27181 tggcaaggcc gaagaattgt ttttagaccc acacaactac taaaacatag ctgtcgtatt
    27241 gacgagaaag gtaaaactat gaaacaggca acaagaaaac cgacgacacc cggagatatt
    27301 cttctctatg aatatctgga accgctcgat ttgaaaatca atgagttagc agagttgctg
    27361 catgttcatc gtaatagcgt cagtgcactg atcaataaca atcgtaaact caccactgag
    27421 atggcattcc gtctggcgaa agtttttgat accacagtcg atttttggct aaacctccag
    27481 gcggcagttg atctttggga agttgaaaac aacatgcgta cccaggaaga attgggacgg
    27541 attgaaacag tggctgaata tttggcacgc cgtgaagagc gtgcaaaaaa ggtcgcgtag
    27601 aataatttcc ctataacgga tgcgccaccc taaagcagtg catccgcaat gatgtactta
    27661 ttccttcggt ttaaaccgca gcaaccggtt ggcgttactc actacggtaa tcgacgagag
    27721 cgccattgcc gctccggcga ccaccgggtt aagcagcgta ccggtgaacg gccacaaaat
    27781 accggcggcg accggaatac caatgctgtt gtagataaac gcaccgagca ggttctgctt
    27841 catattgcgc agcgttgcgc gggaaatcgc gagcgcatcc gcaacaccca tcaggctatg
    27901 gcgcatcagg gtaatcgccg cggtttcaat ggcaacatca ctgccgccac ccattgcaat
    27961 gccgacatcc gcctgagcca gcgctggcgc gtcgttaatg ccatcgccca ccattgccac
    28021 ctgacgtcct tcactttgca gacgtttgat cgcttcggct ttaccgtccg gcagcacccc
    28081 ggctatcacc tcatcaatcc ctgcttcttt ggcgatcgca ttggcggtgg ttgggttatc
    28141 cccggttagc atcaccagac gatatcccgc tttatgcagg cgttgcagcg ccgccacgct
    28201 atcactacgc aatggatcgc gtacagccag cagggctacc gctttcccgt caaccgccag
    28261 cagcacaggc gttgcccctt gcgatgcctg agctgaaata tctgcttcaa tcgctttagt
    28321 atcaacttgc tggtcattta acagcgcctg attgcccagc aataacgcat gaccttcagc
    28381 ttcaccgctc acgcccagcc cgcgcaatgt gcggaaaccg ttgacctgcg gcagctgcat
    28441 atcgcttgct ttatcgagga tcgcccgtgc cagcggatgg ctggaacctt gttccagtgc
    28501 cgccgccaga cgcagcgcct gcgcttcgtc aaaatcagca aatgttttta ctgcgacaac
    28561 ctgcggcttc ccttcggtca gcgtcccggt tttatcgaac accacggtgt cgagcgtact
    28621 ggcgcgttgc agcgcgtcgg cgtcccgcac cagcacgcca aactcagccg cccgcccgac
    28681 gcccgaaata atcgacatcg gcgtcgccag tcccagcgca cacggacagg cgataatcag
    28741 caccgtggtg gcaatcacca gggtataaac aatttgcggt gccgggccaa agaaatacca
    28801 gattgccgca ctgaccagcg caatgactac caccaccggc acaaacacag cggagatttt
    28861 atccgccagt tgaccgattt ctggcttgct gctctgggcc tgtcgcacca tgcgaatgat
    28921 ccgtgacagc gtagtatggc tgccgaccgc actggcgcga aacagcacac tgccgtcctg
    28981 taccactgtc ccggcatgga cgctatcgcc ttcgcctttt tgctgcggga ttggttcgcc
    29041 cgtcagcatc gcttcatcca gccacgcttc gccctgggta atttcaccat ctaccggcac
    29101 ccgatcaccc gtcgtcaggc gcagcaacat acctggctga acatctgcca gcggcacgtt
    29161 tttttcacct tcgttggtga ccaggcgcgc cgtcggtggg gttaaatcga gtaatttctc
    29221 cagcgcctta gaagaacgct gacgtgcgcg cgcttccagc atatgaccga gattaatcag
    29281 accgataatc atcgcactgg cttcatagta aagatgtcgc gcttccatcg ggaaccactg
    29341 cggccacagg ttgacgctca ttgaatagag ccacgccacg cccgtaccca gcgccaccag
    29401 cgtatccatc gtcgctgcac cgttcagcag gcttttccat gcgctgcggt aaaaatggcc
    29461 gccggcgaaa accattaccg ccagggttat caggccgata accaaccaca ggctgcggtt
    29521 gtcggcggtg accatcatgt tatcgccgat catcccccag accatcaccg ggatacccac
    29581 cgccagtgcg acaatcgcct gccagcggaa gcgcttcatc gtagcgacgg cggtttcttg
    29641 ctggcgctcg cggcgtttag cgtcatcttc aatcgcttcc gcgccgtagc ccgctttttc
    29701 caccgcctgc actaaatctt gtggggaggc actgcccatc accagcgcag tacgctccgc
    29761 caggtttacc cgtgcctgag tgacgcccgg tacgctttgc aacgcatttt gtacgcgggt
    29821 gacacagctg gcgcagctca tgccgctcag cagcaactgc tggctgtcat cgtcatcggc
    29881 ggtcgctgcc ggaagcgcct cagaaaccgc tgtcagtgct tccgacggga ttgatgactc
    29941 cgccagcggt ttagcctttg ggtggcttac agatgcgtca taacccgctt gtttgatggt
    30001 ttcgatcagc tgttctgcac tggcagtccc ggtaacgtgc gcttcagtga cagacacatc
    30061 cgcctgctca acatccgggc gctgttcgag actttctttc acgcgtttaa cgcagtgacc
    30121 gcaggacagg ccgtccaggg tcaggtcgat agtttgtgac ataaaacact ccttttaaga
    30181 cagttttgac tggctgtgat aaaggttaaa ccttccagca aggggaaggt caagaaatta
    30241 ataaaccagg cgggtaaaag tacgtaaaga ttaaaaaatc ggctcgattt gcatcaggat
    30301 tagacattta tctctttgtt ttcctgtagt taagttgcgg gtgctaagtt aaatcaggat
    30361 gcctgaaaat tggcaccggg gtgaggaatt acctcccgca tctataaaaa ggagttaaca
    30421 aaagatgtta gatgcaaaca aattacagca ggcagtggat caggcttaca cccaatttca
    30481 ctcacttaac ggcggacaaa atgccgacta cattcccttt ctggcgaatg tacctggtca
    30541 actggcagca gtggctattg tgacctgcga tggcaacatc tatcgcgcgg gtgatagtga
    30601 ttaccgcttt gcgctggaat ccatctcgaa agtctgtacg ttagcccttg cgttagaaga
    30661 tgtcggcccg caggcggtac aggacaaaat tggcgctgac ccgaccggat tgccctttaa
    30721 ctcagttatc gccttagagt tgcatggcgg caaaccgctg tcgccactgg taaatgctgg
    30781 cgctattgcc accaccagcc tgattaacgc tgaaaatact gaacaacgct ggcagcgaat
    30841 tttacatatc caacaacaac tggctggtga gcaggtagcg ctctctgacg aagtcaacca
    30901 gtcggaacaa acaaccaact tccataaccg ggccattgcc tggctgctgt actccgccgg
    30961 atatctctat tgtgatgcaa tggaagcctg tgacgtgtac acccgtcagt gctccacgct
    31021 catcaatact gttgaactgg caacgcttgg cgcgacgctg gcggcgggtg gtgtgaatcc
    31081 gctgacgcat aaacgcgttc ttcaggccga caacgtgccg tacattctgg ctgaaatgat
    31141 gatggaaggg ctgtatggtc gctccggtga ctgggcgtat cgcgttggtt taccgggcaa
    31201 aagcggtgta ggtggcggta ttctggcggt cgtccctggc gtgatgggaa ttgccgcgtt
    31261 ctcaccaccg ctggacgaag aaggcaacag tgttcgcggt caaaaaatgg tggcatcggt
    31321 cgctaagcaa ctcggctata acgtgtttaa gggctgatca tgatgaacac ggaaggtaat
    31381 aacgataaca aacccctcgg tctatggaac gtcgtttcca t
//
LOCUS       DAZFLD010000028        31227 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.028, whole genome shotgun sequence.
ACCESSION   DAZFLD010000028 DAZFLD010000000
VERSION     DAZFLD010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31227
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.028"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            434..523
                     /gene="tisB"
                     /locus_tag="ACHKBL_001128"
     CDS             434..523
                     /gene="tisB"
                     /locus_tag="ACHKBL_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin TisB"
                     /protein_id="HFO5818028.1"
                     /translation="MSLVDIAILILKLIVAALQLLDAVLKYLK"
     gene            complement(647..721)
                     /gene="ysdE"
                     /locus_tag="ACHKBL_001129"
     CDS             complement(647..721)
                     /gene="ysdE"
                     /locus_tag="ACHKBL_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YsdE"
                     /protein_id="HFO5818029.1"
                     /translation="MNVSQIYARNGELFSGRICKQKRQ"
     gene            803..1987
                     /gene="emrD"
                     /locus_tag="ACHKBL_001130"
     CDS             803..1987
                     /gene="emrD"
                     /locus_tag="ACHKBL_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EmrD"
                     /protein_id="HFO5818030.1"
                     /translation="MKRHRNVNLLLMLVLLVAVGQMAQTIYIPAIADMARDLNVREGA
                     VQSVMGAYLLTYGVSQLFYGPISDRVGRRPVILVGMSIFMLATLVAVTTSSLTVLIAA
                     SAMQGMGTGVGGVMARTLPRDLYERTQLRHANSLLNMGILVSPLLAPLIGGLLDTMWN
                     WRACYLFLLVLCAGVTFSMARWMPETRPVDAPRTRLLTSYKTLFGNSGFNCYLLMLIG
                     GLAGIAAFEACSGVLMGAVLGLSSMTVSILFILPIPAAFFGAWFAGRPNKRFSTLMWQ
                     SVICCLLAGLLMWIPDWFGVMNVWTLLIPAALFFFGAGMLFPLATSGAMEPFPFLAGT
                     AGALVGGLQNIGSGVLASLSAMLPQTGQGSLGLLMTLMGLLIVLCWLPLATRMSHQGQ
                     PV"
     gene            complement(1995..2492)
                     /gene="yidF"
                     /locus_tag="ACHKBL_001131"
     CDS             complement(1995..2492)
                     /gene="yidF"
                     /locus_tag="ACHKBL_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HFO5818031.1"
                     /translation="MTGSQVIDAEEDRHKLVVEYKDALQPADFYHNFKQLGIRSVQLI
                     PHLEFDDRGDLTAASVTAELWGKFLIALFECWVRADISRISIELFDTTLQKWCGSEKS
                     QPRRDCQACDWHRLCPHAREDKPDNVLCAGYQAFYSYSAPHMRVMRDLIKQHRSPMEL
                     MTMLR"
     gene            complement(2489..2851)
                     /gene="yidG"
                     /locus_tag="ACHKBL_001132"
     CDS             complement(2489..2851)
                     /gene="yidG"
                     /locus_tag="ACHKBL_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HFO5818032.1"
                     /translation="MPDSRKARRIADPGLQPERTSLAWFRTMLGYGALMALAIKHNWH
                     QAGMLFWISIGILAIVALILWHYTRNRNLMDVTNSDFSQFHVVRDKFLISLAVLSLAI
                     LFAVTHIHQLIVFIERVA"
     gene            complement(2841..3188)
                     /gene="yidH"
                     /locus_tag="ACHKBL_001133"
     CDS             complement(2841..3188)
                     /gene="yidH"
                     /locus_tag="ACHKBL_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidH family protein"
                     /protein_id="HFO5818033.1"
                     /translation="MKISRLGEAPDYRFSLANERTFLAWIRTALGFLAAGVGLDQLAP
                     DFATPVIRELLALLLCLFSGGLAMYGYLRWLRNEKAMRLKEDLPYTNSLLIISLILMV
                     VAVIVMGLVLYAG"
     gene            complement(3248..4741)
                     /gene="yidJ"
                     /locus_tag="ACHKBL_001134"
     CDS             complement(3248..4741)
                     /gene="yidJ"
                     /locus_tag="ACHKBL_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFO5818034.1"
                     /translation="MKRPNFLFIMTDTQATNMVGCYSGKPLNTQNIDSLAAESIRFNS
                     AYTCSPVCTPARAGLFTGIYANQSGPWTNNVAPGKNISTMGRYFKDAGYHTCYIGKWH
                     LDGHDYFGTGECPPEWDADYWFDGANYLSELTEKEISLWRNGLNSVEDLQANHIDETF
                     TWAHRISNRAVDFLQQPARADEPFLMVVSYDEPHHPFTCPVEYLEKYADFYYELGEKS
                     QDDLANKPEHHRLWAQAMPSPVGDDGLYHHPLYFACNDFVDDQIGRVINALTPEQREN
                     TWVIYTSDHGEMMGAHKLISKGAAMYDDITRIPLIIRSPQGERRQVDTPVSHIDLLPT
                     MMALADIEKPEILPGDNILAVKEPRGVLVEFNRYEIEHDSFGGFIPVRCWVTDDFKLV
                     LNLFTSDELYDRRNDPNEMHNLIDDIHFADVRSKMHDALLDYMDKIRDPFRSYQWNLR
                     PWRKDAQPRWMGAFRPRPQDGYSPVVRDYDTGLPTQGVKVEEKKQKF"
     gene            complement(4738..6453)
                     /gene="yidK"
                     /locus_tag="ACHKBL_001135"
     CDS             complement(4738..6453)
                     /gene="yidK"
                     /locus_tag="ACHKBL_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
                     /protein_id="HFO5818035.1"
                     /translation="MNSLQILSFVGFTLLVAVITWWKVRKTDTGSQQGYFLAGRSLKA
                     PVIAASLMLTNLSTEQLVGLSGQAYKSGMSVMGWEVTSAVTLIFLALIFLPRYLKRGI
                     ATIPDFLEERYDKTTRIIIDFCFLIATGVCFLPIVLYSGALALNSLFHVGESLQLSDG
                     AAIWLLVILLGLAGILYAVIGGLRAMAVADSINGIGLVIGGLMVPVFGLIAMGKGSFM
                     QGIEQLTTVHAEKLNSVGGPTDPLPIGAAFTGLILVNTFYWCTNQGIVQRTLASKSLA
                     EGQKGALLTAVLKMLDPLVLVLPGLIAFHLYQDLPKADMAYPTLVNNVLPVPLVGFFG
                     AVLFGAVISTFNGFLNSASTLFSMGIYRRIINQNAEPQQLVTVGRKFGFFIAIVSVLV
                     APWIANAPQGLYSWMKQLNGIYNVPLVTIIIMGFFFPRIPALAAKVAMGIGIISYITI
                     NYLVKFDFHFLYVLACTFCINVVVMLVIGFIKPRATPFTFKDAFAVDMKPWKNVKIAS
                     MGILFAMIGVYAGLAEFGGYGTRWLAMISYFIAAVVIVYLIFDSWRHRHDPAVTFTPD
                     AKDSL"
     gene            6620..7486
                     /gene="yidL"
                     /locus_tag="ACHKBL_001136"
     CDS             6620..7486
                     /gene="yidL"
                     /locus_tag="ACHKBL_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFO5818036.1"
                     /translation="MNGKLQSSDVKNETPYNIPLLINENVISSGISLISLWHTYADEH
                     YRVIWPRDKKKPLIANSWVAVYTVQGCGKILLKNDEQITLHGNCIIFLKPMDIHSYHC
                     EGLVWEQYWMEFTPTSMMDIPVGQQSVIYNGEVYNQELTEVAELITSPEAIKNNLAVA
                     FLTKIIYQWICLMHAVGKKDPQRRQIEKLIATLHASLQQRWSVADMAATIPCSEAWLR
                     RLFLRYTGKTPKEYYLDARLDLALSLLKQQGNSVGEVADTLNFFDSFHFSKAFKHKFG
                     YAPSAVLKNTDR"
     gene            complement(7576..9237)
                     /gene="yidE"
                     /locus_tag="ACHKBL_001137"
     CDS             complement(7576..9237)
                     /gene="yidE"
                     /locus_tag="ACHKBL_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HFO5818037.1"
                     /translation="MSDIALTVSILALVAVVGLFIGNVKFRGVGLGIGGVLFGGIIVG
                     HFVSQAGMTLSSDMLHVIQEFGLILFVYTIGIQVGPGFFASLRVSGLRLNLFAVLIVI
                     IGGLVTAILHKLFDIPLPVVLGIFSGAVTNTPALGAGQQILRDLGTPMAMVDQMGMSY
                     AMAYPFGICGILFTMWMLRVIFRVNVETEAQQHESTRTNGGALIRTINIRVENPNLHN
                     LAIKDVPILNGDKVICSRLKREETLKVPSPETVIQLGDLLHLVGQPADLHNAQLVIGQ
                     EVDTSLSTKGTDLRVERVVVTNENVLGKRIRDLHFKERYDVVISRLNRAGVELVASSD
                     ISLQFGDILNLVGRPSAIDAVANVLGNAQQKLQQVQMLPVFIGIGLGVLLGSIPVFVP
                     GFPAALKLGLAGGPLIMALILGRIGSIGKLYWFMPPSANLALRELGIVLFLSVVGLKS
                     GGDFIHTLVDGEGLSWIGYGALITAVPLITVGILARMLAKMNYLTMCGMLAGSMTDPP
                     ALAFANNLHPTSGAAALSYATVYPLVMFLRIITPQLLAVLFWSIG"
     gene            complement(9433..9861)
                     /gene="ibpB"
                     /locus_tag="ACHKBL_001138"
     CDS             complement(9433..9861)
                     /gene="ibpB"
                     /locus_tag="ACHKBL_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small heat shock chaperone IbpB"
                     /protein_id="HFO5818038.1"
                     /translation="MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNH
                     YRITLALAGFRQEDLEIQLEGTRLSVKGTPEQPKEEKKWLHQGLMNQPFSLSFTLAEN
                     MEVSGATFVNGLLHIDLIRNEPEPIAAQRIAISERPALNS"
     gene            complement(9973..10386)
                     /gene="ibpA"
                     /locus_tag="ACHKBL_001139"
     CDS             complement(9973..10386)
                     /gene="ibpA"
                     /locus_tag="ACHKBL_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small heat shock chaperone IbpA"
                     /protein_id="HFO5818039.1"
                     /translation="MRNFDLSPLYRSAIGFDRLFNHLENNQSQSNGGYPPYNVELVDE
                     NHYRIAIAVAGFAESELEITAQDNLLVVKGAHADEQKERTYLYQGIAERNFERKFQLA
                     ENIHVRGANLVNGLLYIDLERVIPEAKKPRRIEIN"
     misc_feature    complement(10380..10452)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01832"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ROSE (Repression Of Heat Shock gene Expression)
                     occurs in the 5'-region of heat shock genes and acts as an
                     RNA thermometer to modulate expression; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF01832"
     gene            10691..11023
                     /gene="yidQ"
                     /locus_tag="ACHKBL_001140"
     CDS             10691..11023
                     /gene="yidQ"
                     /locus_tag="ACHKBL_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HFO5818040.1"
                     /translation="MIRNVLLAFMICSGMTLLGGCSSVMSHTGGKEGTYPGTRASATM
                     IGDDETNWGTKSLAILDMPFTAVMDTLLLPWDVFRKDSSVRSRVEKSEANAQATNAVI
                     PPARMPDN"
     gene            complement(11025..12239)
                     /gene="yidR"
                     /locus_tag="ACHKBL_001141"
     CDS             complement(11025..12239)
                     /gene="yidR"
                     /locus_tag="ACHKBL_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312656.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
                     /protein_id="HFO5818041.1"
                     /translation="MKQITFAPRNHLLTNTNTWTPDSQWLVFDVRPSGASFTGETIER
                     VNIHTGEVEVIYRASQGAYVGVVTVHPKSEKYVFIHGPENPDETWYYDFHHRRGVIAE
                     GGKVSNLDAMDITAPYTPGALRGGSHVHVFSPNGERVSFTYNDHVMHQLDSTLDLRNV
                     GVAAPFGPVNVQKQHPREYSGSHWCVLVSKTTPTPQPGSNEINRAYEEGWVGNHALAF
                     IGDTLSPKGEKVPELFIVELPQDEAGWKAAGDAPLSGTETTLPAPPRGVVQRRLTFTH
                     HRAYPGLVNVPRHWVRCNPQGTQIAFLMRDDNGIVQLWLISPQGGEPRQLTHNKTDIQ
                     SAFNWHPSGEWLGFVLDNRIACAHAQSGEVEYLTENHANPPSADAVVFSPDGQWLAWM
                     EGGQLWITETDR"
     gene            12340..13404
                     /gene="cbrA"
                     /locus_tag="ACHKBL_001142"
     CDS             12340..13404
                     /gene="cbrA"
                     /locus_tag="ACHKBL_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin M resistance lipid reductase CbrA"
                     /protein_id="HFO5818042.1"
                     /translation="MEHFDVAIIGLGPAGSALARKLAGKMQVIALDKKHQHGTEGFSK
                     PCGGLLAPDAQRSFIRDGLTLPVDVIANPQIFSVKTVDVAASLTRNYQRSYININRHA
                     FDLWMKSLIPASVEVYHDSLCRKIWREDDKWHVIFRADGWEQHITARYLVGADSANSM
                     VRRHLYPDHQIRKYVAIQQWFAEKHPVPFYSCIFDNAITDCYSWSISKDGYFIFGGAY
                     PMKDGQTRFTMLKEKMSAFQFQFGKAVKSEKCTVLFPSRWQDFVCGKDNAFLIGEAAG
                     FISASSLEGISYALDSAEILRSVLLKQPEKSNAAYWRATRKLRLKLFGKIVKSRCLTA
                     PALRKWIMRSGVAHIPQLKD"
     gene            complement(13401..14738)
                     /gene="dgoT"
                     /locus_tag="ACHKBL_001143"
     CDS             complement(13401..14738)
                     /gene="dgoT"
                     /locus_tag="ACHKBL_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO5818043.1"
                     /translation="MVSGFAMPKIWRKLAMDIPVNAAKPGRRRYLTLVMIFITVVICY
                     VDRANLAVASAHIQEEFGITKAEMGYVFSAFAWLYTLCQIPGGWFLDRVGSRVTYFIA
                     IFGWSVATLFQGFATGLMSLIGLRAITGIFEAPAFPTNNRMVTSWFPEHERASAVGFY
                     TSGQFVGLAFLTPLLIWIQEMLSWHWVFIVTGGIGIIWSLIWFKVYQPPRLTKGISKA
                     ELDYIRDGGGLVDGDAPVKKEARQPLTAKDWKLVFHRKLIGVYLGQFAVASTLWFFLT
                     WFPNYLTQEKGITALKAGFMTTVPFLAAFVGVLLSGWVADLLVRKGFSLGFARKTPII
                     CGLLISTCIMGANYTNDPMMIMCLMALAFFGNGFASITWSLVSSLAPMRLIGLTGGVF
                     NFAGGLGGITVPLVVGYLAQGYGFAPALVYISAVALIGALSYILLVGDVKRVG"
     gene            complement(14813..15961)
                     /gene="dgoD"
                     /locus_tag="ACHKBL_001144"
     CDS             complement(14813..15961)
                     /gene="dgoD"
                     /locus_tag="ACHKBL_001144"
                     /EC_number="4.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008869 - galactonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
                     /protein_id="HFO5818044.1"
                     /translation="MKITKITTYRLPPRWMFLKIETDEGVVGWGEPVIEGRARTVEAA
                     VHELGDYLIGQDPSRINDLWQVMYRAGFYRGGPILMSAIAGIDQALWDIKGKVLNAPV
                     WQLMGGLVRDKIKAYSWVGGDRPADVIDGIKTLREIGFDTFKLNGCEELGLIDNSRAV
                     DAAVNTVAQIREAFGNQIEFGLDFHGRVSAPMAKVLIKELEPYRPLFIEEPVLAEQAE
                     YYPKLAAQTHIPLAAGERMFSRFDFKRVLEAGGISILQPDLSHAGGITECYKIAGMAE
                     AYDVTLAPHCPLGPIALAACLHIDFVSYNAVLQEQSMGIHYNKGAELLDFVKNKEDFS
                     MVGGFFKPLTKPGLGVEIDEAKVIEFSKNAPDWRNPLWRHEDNSVAEW"
     gene            complement(15958..16575)
                     /gene="dgoA"
                     /locus_tag="ACHKBL_001145"
     CDS             complement(15958..16575)
                     /gene="dgoA"
                     /locus_tag="ACHKBL_001145"
                     /EC_number="4.1.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HFO5818045.1"
                     /translation="MQWQTKLPLIAILRGITPDEALAHVGAVIDAGFDAVEIPLNSPQ
                     WEKSIPAIVDAYGDKALIGAGTVLKPEQVDALARMSCQLIVTPNIHSEVIRRAVGYGM
                     TVCPGCATATEAFTALDAGAQALKIFPSSAFGPQYIKALKAVLPPDIAVFAVGGVTPE
                     NLAQWIDAGCAGAGLGSDLYRAGQSVERTAQQAAAFVKAYREAVQ"
     gene            complement(16559..17437)
                     /gene="dgoK"
                     /locus_tag="ACHKBL_001146"
     CDS             complement(16559..17437)
                     /gene="dgoK"
                     /locus_tag="ACHKBL_001146"
                     /EC_number="2.7.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HFO5818046.1"
                     /translation="MTARYIAIDWGSTNLRAWLYQGDHCLDSRQSEAGVTRLNGKSPA
                     AVLAEVTTDWREENTPVVMAGMVGSNVGWKVAPYLSVPARFSSIGDQLTSVGDNIWII
                     PGLCVSHDDNHNVMRGEETQLIGARALAPSSLYVMPGTHCKWVQADSQQINDFRTVMT
                     GELHHLLLNHSLIGAGLPPQENSADAFAAGLERGFNAPAILPQLFEVRASHVLGTLPR
                     EQVSEFLSGLLIGAEVASMRDYVTHQHAITLVAGTSLTARYQQAFQAMGCDVTAVAGD
                     TAFQAGIRSIAHAVAN"
     gene            complement(17434..18123)
                     /gene="dgoR"
                     /locus_tag="ACHKBL_001147"
     CDS             complement(17434..18123)
                     /gene="dgoR"
                     /locus_tag="ACHKBL_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate utilization transcriptional
                     regulator DgoR"
                     /protein_id="HFO5818047.1"
                     /translation="MTLNKTDRIVITLGKQIVHGKYVPGSPLPAEAELCEEFATSRNI
                     IREVFRSLMAKRLIEMKRYRGAFVAPRNQWNYLDTDVLQWVLENDYDPRLISAMSEVR
                     NLVEPAIARWAAERATSSDLAQIESALNEMIANNQDREAFNEADIRYHEAVLQSVHNP
                     VLQQLSIAISSLQRAVFERTWMGDEANMPQTLQEHKALFDAIRHQDGDAAEQAALTMI
                     ASSTRRLKEIT"
     gene            18400..19056
                     /locus_tag="ACHKBL_001148"
     CDS             18400..19056
                     /locus_tag="ACHKBL_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010377884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5818048.1"
                     /translation="MKLNFKGFFKAAGLCSLAFALTGCITWGLVSNTASRPHAEQWRS
                     DTIKGLSLAEDSNGTKGYVFVGESLDYLLTTGGDEVVKMLNDPAIHGERITVSDNAKF
                     ILSSSNKNFSGAITLYYDWNNEEDKALATQYGFICDTRRCTWMLDGLKGSIHQKNKKA
                     DYSNVMVFHQPFTVGFYEYKATDGVPRGLVNALLPVTLTLDIVTSPLQFLILCTTRNC
                     "
     gene            complement(19100..19912)
                     /gene="yidA"
                     /locus_tag="ACHKBL_001149"
     CDS             complement(19100..19912)
                     /gene="yidA"
                     /locus_tag="ACHKBL_001149"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HFO5818049.1"
                     /translation="MAIKLIAIDMDGTLLLPDHTISPAVKNAIAAARARGVNVVLTTG
                     RPYAGVHNYLKELHMEQPGDYCITYNGALVQKAADGSTVAQTTLSYDDYRFLEKLSRE
                     VGSHFHALDRTTLYTANRDISYYTVHESFVATIPLVFCEAEKMDPNTQFLKVMMIDEP
                     AILDQAIARIPQEVKEKYTVLKSAPYFLEILDKRVNKGTGVKSLADVLGIKPEEIMAI
                     GDQENDIAMIEYAGVGVAMDNAIPSVKEVANFVTKSNLEDGVAFAIEKYVLN"
     gene            complement(20027..20425)
                     /gene="yidB"
                     /locus_tag="ACHKBL_001150"
     CDS             complement(20027..20425)
                     /gene="yidB"
                     /locus_tag="ACHKBL_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HFO5818050.1"
                     /translation="MGLFDDVVGAFLKGDAGKYQAILSWVEEQGGIQVLLEKLQSGGL
                     GAILSTWLSNQQGNQPVSGEQLESALGTNAVSDLGQKLGVDTSTASSLLAEQLPKIID
                     ALSPQGEVSPQANNDLLSAGMELLKGKLFR"
     gene            20543..20644
                     /locus_tag="ACHKBL_001151"
                     /pseudo
     CDS             20543..20644
                     /locus_tag="ACHKBL_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(20665..23079)
                     /gene="gyrB"
                     /locus_tag="ACHKBL_001152"
     CDS             complement(20665..23079)
                     /gene="gyrB"
                     /locus_tag="ACHKBL_001152"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HFO5818051.1"
                     /translation="MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVV
                     DNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGG
                     KFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGET
                     EKTGTMVRFWPSLETFTNVTEFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHY
                     EGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQR
                     DGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSS
                     QTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMT
                     RRKGALDLAGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKI
                     LNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHSIIIMTDADVDGSHIRT
                     LLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIALDGATL
                     HTNASAPALAGEALEKLVSEYNATQKMINRMERRYPKAMLKELIYQPTLTEADLSDEQ
                     TVTRWVNALVSELNDKEQHGSQWKFDVHTNAEQNLFEPIVRVRTHGVDTDYPLDHEFI
                     TGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFEQALDWLVKESRRGLSIQRYK
                     GLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADQLFTTLMGDAVEPRRAFIEENALK
                     AANIDI"
     gene            complement(23108..24181)
                     /gene="recF"
                     /locus_tag="ACHKBL_001153"
     CDS             complement(23108..24181)
                     /gene="recF"
                     /locus_tag="ACHKBL_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HFO5818052.1"
                     /translation="MSLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIY
                     TLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDG
                     HKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWGCFHNEPGFFTAWSNLKRLLKQ
                     RNAALRQVTRYEQLRPWDKELIPLAEQISTWRAEYSAGIAADMADTCKQFLPEFSLTF
                     SFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKL
                     LMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISA
                     EHVIDMSDENSKMFTVEKGKITD"
     gene            complement(24181..25281)
                     /gene="dnaN"
                     /locus_tag="ACHKBL_001154"
     CDS             complement(24181..25281)
                     /gene="dnaN"
                     /locus_tag="ACHKBL_001154"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006177590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HFO5818053.1"
                     /translation="MKFTVEREHLLKPLQQVSGPLGGRPTLPILGNLLLQVADGTLSL
                     TGTDLEMEMVARVALVQPHEPGATTVPARKFFDICRGLPEGAEIAVQLEGERMLVRSG
                     RSRFSLSTLPAADFPNLDDWQSEVEFTLPQATMKRLIEATQFSMAHQDVRYYLNGMLF
                     ETEGEELRTVATDGHRLAVCSMPIGQSLPSHSVIVPRKGVIELMRMLDGGDNPLRVQI
                     GSNNIRAHVGDFIFTSKLVDGRFPDYRRVLPKNPDKHLEAGCDLLKQAFARAAILSNE
                     KFRGVRLYVSENQLKITANNPEQEEAEEILDVTYSGAEMEIGFNVSYVLDVLNALKCE
                     NVRMMLTDSVSSVQIEDAASQSAAYVVMPMRL"
     gene            complement(25286..26689)
                     /gene="dnaA"
                     /locus_tag="ACHKBL_001155"
     CDS             complement(25286..26689)
                     /gene="dnaA"
                     /locus_tag="ACHKBL_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HFO5818054.1"
                     /translation="MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLALYAPN
                     RFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGTKPVTQTPQAAVTSNVAAPAQVA
                     QTQPQRAAPSTRSGWDNVPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAARQVADN
                     PGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNA
                     IEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEIN
                     GVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEVAFFIAKRLRSNVR
                     ELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVA
                     DLLSKRRSRSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREE
                     SHDIKEDFSNLIRTLSS"
     gene            26986..27066
                     /locus_tag="ACHKBL_001156"
     CDS             26986..27066
                     /locus_tag="ACHKBL_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5818055.1"
                     /translation="MTIDKNWLNRSNKDPGRSLRFTHQPV"
     gene            27296..27436
                     /gene="rpmH"
                     /locus_tag="ACHKBL_001157"
     CDS             27296..27436
                     /gene="rpmH"
                     /locus_tag="ACHKBL_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HFO5818056.1"
                     /translation="MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTV
                     SK"
     gene            27453..27812
                     /gene="rnpA"
                     /locus_tag="ACHKBL_001158"
     CDS             27453..27812
                     /gene="rnpA"
                     /locus_tag="ACHKBL_001158"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFO5818057.1"
                     /translation="MVKLAFPRELRLLTPSQFTFVFQQPQRAGTPQITILGRLNSLGH
                     PRIGLTVAKKNVRRAHERNRIKRLTRESFRLRQHELPAMDFVVVAKKGVADLDNRALS
                     EALEKLWRRHCRLARGS"
     gene            27776..28033
                     /gene="yidD"
                     /locus_tag="ACHKBL_001159"
     CDS             27776..28033
                     /gene="yidD"
                     /locus_tag="ACHKBL_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HFO5818058.1"
                     /translation="MAPPLSPGSRVLIALIRVYQRLISPLLGPHCRFTPTCSSYGIEA
                     LRRFGVIKGSWLTVKRVLKCHPLHPGGDDPVPPGPFDTREH"
     gene            28036..29682
                     /gene="yidC"
                     /locus_tag="ACHKBL_001160"
     CDS             28036..29682
                     /gene="yidC"
                     /locus_tag="ACHKBL_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HFO5818059.1"
                     /translation="MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAA
                     GSAADQGVPASGQGKLISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLET
                     SPQFIYQAQSGLTGRDGPDNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNT
                     FTKTFVLKRGDYAVNVNYNVQNAGEKPLEISSFGQLKQSITLPPHLDTGSSNFALHTF
                     RGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYT
                     ANLGNGIAAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLW
                     FISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKAQYTSMAKMRMLQPKIQA
                     MRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVELRQAP
                     FALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLW
                     FPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS"
     gene            29788..31152
                     /gene="mnmE"
                     /locus_tag="ACHKBL_001161"
     CDS             29788..31152
                     /gene="mnmE"
                     /locus_tag="ACHKBL_001161"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HFO5818060.1"
                     /translation="MSDNDTIVAQATPPGRGGVGILRISGFKAREVAETVLGKLPKPR
                     YADYLPFKDADGSVLDQGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPG
                     LRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
                     EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNDVIADLDAVRAEARQGSLLREGMK
                     VVIAGRPNAGKSSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRE
                     ASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEFIARLPAKLPITVVR
                     NKADITGETLGMSEVNGHALIRLSARTGEGVDVLRNHLKQSMGFDTNMEGGFLARRRH
                     LQALEQAAEHLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDDLLGRIFSSF
                     CIGK"
BASE COUNT         7474 a         8371 c         8005 g         7377 t
ORIGIN      
        1 aaaaaccccg ccggagcgag gtttcgtcag tcgcctgcgg ctggtaaccg caaagcacac
       61 tgtattatgt caacactgaa agtatacgtg ttccgcgcag aacgcgcaat ttcggcacga
      121 attttgacgt atttagtgca tagttgagta tcgatcacag tttgcgtttt gtccaaaatt
      181 actgtttatt tatacagtaa acttctataa tatcactgta cgcaatgtgt tatgcggggg
      241 ccgcatcgtt acccggcgca ctaagtcctg gcagaaacgg gtggtgccgt cagcgcctta
      301 accccgcgtg agcacactgt gttatgtcaa caagcacaac gtttctcctt gagataccgc
      361 gtgcacaaca gctggcaaca ggcagcggaa aggtacgtca gctggcagtg ctcctgaacc
      421 acaggagacg cgtatgagcc tggtggatat cgccattctt atcctcaaac tcattgttgc
      481 agcactgcaa ctgcttgatg ctgttctgaa atacctgaag taattcagat tcaagtcgca
      541 ctaaagggga gcgggtaacc gctccccttt tatatttagc gtgcgggttg gtgtcggatg
      601 cgatgctgac gcatcttatc cgccctacca tctctcccgg caacatttat tgccgctttt
      661 gtttacatat tctgccgcta aacaattccc cattcctggc gtatatctgg ctaacattca
      721 tcaatgtgat agattcctct cccgcattta tgggaatgcg tagtgactta ttctaattat
      781 ttttataaaa gcatccgtga taatgaaaag gcatagaaac gtcaatttgt tattgatgtt
      841 ggtattactc gtggccgtcg gtcagatggc gcaaaccatt tatattccag ctattgccga
      901 tatggcgcgc gatctcaacg tccgtgaagg ggcggtgcag agcgtaatgg gcgcttatct
      961 gctgacttac ggtgtctcac agctgtttta tggcccgatt tccgaccgcg tgggtcgccg
     1021 accggtgatc ctcgtcggaa tgtccatttt tatgctggca acgctggtcg cggtcacgac
     1081 ctccagtttg acggtattga ttgccgccag cgcgatgcag gggatgggca ccggcgttgg
     1141 cggcgtaatg gcgcgtactt tgccgcgcga tttatatgaa cggacacagt tgcgccatgc
     1201 taacagcctg ttaaacatgg ggattcttgt cagtccgttg ctcgcaccgc taatcggcgg
     1261 tctgctggat acgatgtgga actggcgtgc ctgttatctc tttttgttgg ttctttgtgc
     1321 cggtgtgact ttcagtatgg cccgctggat gccggaaacg cgtccggtcg acgcaccgcg
     1381 cacgcgcctg cttaccagtt ataaaacgct tttcggtaac agcggtttta actgttattt
     1441 gctgatgctg attggcggtc tggccggaat tgccgccttt gaagcctgct ccggcgtgct
     1501 gatgggcgcg gtgttagggc tgagcagtat gacggtcagt attttgttta ttctgccgat
     1561 tccggcggca ttttttggcg catggtttgc cggacgtccc aataaacgct tctcaacgtt
     1621 gatgtggcag tcggttatct gttgcctgct ggctggcttg ctgatgtgga tccccgactg
     1681 gtttggcgtg atgaatgtct ggacgctgct cattcccgcc gcgctgttct ttttcggtgc
     1741 cgggatgctg tttccactgg ctaccagcgg cgcgatggag ccgttcccct tcctggcggg
     1801 caccgctggc gcgctggtcg gcggtctgca aaacattggt tccggcgtgc tggcgtcgct
     1861 ctctgcgatg ttgccgcaaa ccggtcaggg cagcctggga ttgttgatga ccttaatggg
     1921 attgttgatc gtgctgtgct ggctaccgct ggcgacgcgg atgtcgcatc aggggcagcc
     1981 tgtttaagct cacgtcaccg cagcatcgtc atcagctcca tgggagaacg atgctgcttt
     2041 atcagatcac gcatcacccg catatgcggc gcggagtaag aataaaacgc ctgatagccc
     2101 gcacaaagca cgttgtctgg tttatcttcc cgagcgtgcg ggcacagccg atgccagtcg
     2161 cacgcctggc agtcacggcg cggttgcgac ttttcgctgc cacaccattt ttgtaaggtg
     2221 gtgtcaaaaa gttcaatcga aatacgactg atatctgccc gcacccaaca ctcgaacagg
     2281 gcaattaaaa atttccccca cagctctgct gtcaccgaag cggccgtcag atcgccccgg
     2341 tcatcaaatt caagatgggg aataagctgt actgagcgga tgcccagctg tttgaaattg
     2401 tgataaaaat cggcaggttg cagagcatcc ttatactcta caaccagttt atgcctgtcc
     2461 tcttcagcat caatgacctg acttcctgtc atgcgactct ctcaataaat acgataagtt
     2521 gatgtatatg cgttacagca aacagtattg cgagagataa caccgcgagg gagatcaaaa
     2581 atttgtcacg gactacgtga aattgggaaa aatcgctatt cgtgacatcc attaaattgc
     2641 gattacgggt gtagtgccag aggatcagcg ccacgatggc gaggatgcca atcgaaatcc
     2701 agaataacat gcccgcctgg tgccagttgt gtttgatagc caacgccatc aacgcgccgt
     2761 agcccagcat ggtacgaaac cacgccagtg atgtgcgttc cggctgcagc cctgggtcgg
     2821 caatgcgtct ggctttgcgg ctatccggca tacaacacca gtcccatgac gatcactgcc
     2881 acgaccatca atattaaact gatgattaat aagctgttgg tatagggcag gtcctctttc
     2941 aggcgcatcg ctttttcatt gcgtaaccag cgcaagtagc catacatcgc taacccgccg
     3001 gaaaacagac acaacaacaa cgccagcagt tcgcggatga cgggcgtggc gaaatctggc
     3061 gcaagttgat caagcccaac ccccgccgcc agaaatccta gtgcagtacg gatccacgcc
     3121 agaaaagtac gctcattagc cagcgagaag cggtaatcgg gcgcttctcc gaggcgggaa
     3181 attttcattg ttgctccttg taaactgttc ctcgccgaaa aattaggcca ggaacagcaa
     3241 taatgcatca gaacttctgt tttttctctt ccaccttcac cccttgtgtc ggtaaaccgg
     3301 tgtcatagtc gcgtaccact ggagaataac catcctgtgg gcgtggacga aacgccccca
     3361 tccagcgcgg ttgtgcatct ttgcgccacg gacgcaggtt ccattgataa ctgcggaacg
     3421 gatcgcgaat tttgtccata taatccaata aggcgtcatg cattttgctg cgaacgtcgg
     3481 caaaatggat atcatcgatc aggttatgca tttcatttgg gtcattacgg cgatcgtaaa
     3541 gttcatcact ggtgaagagg ttgagcacca gtttaaagtc atccgttacc cagcaacgca
     3601 ccggaataaa accgccaaag ctgtcatgct caatctcgta gcgattaaat tccaccaaca
     3661 cgccacgtgg ttcttttacg gcaaggatat tatcccccgg cagaatctct ggtttttcaa
     3721 tatctgccag cgccatcatt gtcggcagta agtcaatatg actgactggc gtatcgacct
     3781 gtcgccgctc cccttgcggc gaacggatga tcagcggaat gcgggtgatg tcgtcataca
     3841 tcgccgcccc tttactgatc agcttatgtg cgcccatcat ttcaccatga tcggaggtat
     3901 aaatgaccca cgtattttca cgttgctctg gcgttaaggc attgatgaca cgtccgattt
     3961 ggtcatcaac aaagtcattg caggcaaaat agagcggatg gtgataaagc ccgtcatcac
     4021 cgactggcga tggcatcgcc tgcgcccata agcggtgatg ttccggtttg ttcgccaggt
     4081 catcctgtga tttctcgcca agttcgtagt aaaaatcagc gtatttctct aaatactcca
     4141 ccggacaggt gaacgggtga tgcggctcat catacgaaac caccatcagg aaaggttcgt
     4201 cggcgcgcgc gggctgttgc agaaaatcta ccgcccgatt gctgatgcgg tgcgcccagg
     4261 tgaaggtttc gtcgatatgg ttcgcctgta aatcctcaac gctgtttagg ccattgcgcc
     4321 acaggctgat ctctttttcc gtcagttcgc taaggtagtt cgccccatcg aaccagtaat
     4381 cagcgtccca ctccggcgga cactcgccag tgccgaaata gtcatgaccg tcgagatgcc
     4441 atttgccaat gtaacaggtg tgatagcccg catccttaaa gtagcgcccc atggtggaaa
     4501 tgtttttacc tggcgcgacg ttgttggtcc acgggccgga ctggttagcg tagataccgg
     4561 taaacagccc ggcgcgcgct ggtgtacaaa ccggtgaaca ggtataggcg gaattaaagc
     4621 gaatactttc cgccgccaga ctatcaatat tttgcgtatt cagcggctta ccgctatagc
     4681 aaccgaccat attggtggcc tgggtatcgg tcatgatgaa cagaaaattg gggcgtttca
     4741 taggctatcc ttcgcgtcgg gagtaaaggt tacggctggg tcgtgacgat gccgccagct
     4801 atcaaaaatc aggtagacaa tcactacggc ggcaatgaaa taactgatca tcgctaacca
     4861 gcgcgtaccg tagccgccga attcagccag cccggcatag acgccaatca tcgcgaacag
     4921 gatgcccatt gacgcgatct tgacgttttt ccacggtttc atgtccaccg caaacgcatc
     4981 tttgaaggtg aacggcgtgg cgcgcggttt gataaaaccg atcaccagca tcacgaccac
     5041 gttgatgcag aacgtacagg ccagcacata gaggaaatgg aagtcgaact tcaccagata
     5101 gttgatggtg atgtagctga ttatgccaat ccccatcgcc acttttgccg ccagcgccgg
     5161 gatgcgcggg aagaaaaagc ccataatgat gatggtaacc agcggcacgt tgtaaatgcc
     5221 gttgagctgt ttcatccagc tatacagccc ctgcggcgcg ttggcgatcc acggcgctac
     5281 cagcaccgaa acgatggcga taaagaaacc aaatttgcgc ccgacggtga ccagctgctg
     5341 cggctcggca ttctggttaa tgatgcgccg gtaaataccc atactgaata acgtactggc
     5401 gctattcaga aagccgttga aggtactgat caccgcacca aataacaccg cgccgaagaa
     5461 acccaccagt ggcactggca gaacgttatt gaccagcgtc gggtaggcca tgtcggcttt
     5521 cggtagatcc tgatacagat gaaacgcaat caaccctggc agcaccagta ccagcgggtc
     5581 gagcattttc agcaccgccg ttaacagcgc ccccttttgc ccttccgcca ggctttttga
     5641 cgccagcgtg cgttgcacga tgccctgatt tgtacaccag taaaaggtgt tcaccagaat
     5701 caaaccggta aatgccgcgc caatcggcaa gggatcggtc gggccgccga ctgagtttaa
     5761 tttctcggcg tgaacggtgg tgagttgctc aatgccctgc ataaagctgc ccttgcccat
     5821 cgcgatcagg ccaaataccg gcaccatcaa cccgccaata accagcccaa taccgttaat
     5881 ggagtccgcc actgccattg cgcgcagtcc gccgatcacc gcatacaaaa ttcccgccag
     5941 accaagcaaa ataaccagta accagatagc cgcaccatcg gaaagttgta gcgactcacc
     6001 cacatgaaac aggctgttga gcgccaacgc gccggaatag agaacaatcg gcagaaagca
     6061 gacgccggtg gcaatgagga agcagaagtc gatgataata cgcgtcgttt tatcataacg
     6121 ttcctccaga aaatcgggga tggtggcaat gccgcgcttc agatagcgcg gtaaaaagat
     6181 tagcgcgagg aagatcagcg tcactgcaga ggtgacttcc cagcccatca ccgacatgcc
     6241 acttttgtag gcctgcccag aaaggccgac cagttgttcc gtggaaaggt tggttagcat
     6301 taacgaagcg gcaataaccg gcgcttttag tgaacgtccg gcaagaaaat agccttgttg
     6361 cgatccggta tctgttttac ggaccttcca ccaggtgatg accgctacca gcagcgtaaa
     6421 accgacaaaa ctcaagattt gtaacgaatt catctcatag cccttattta tgtttatgtc
     6481 ccggacgcat gaatataggg agaggtaaaa tgcgtcgggt caggcagtca gattcttaaa
     6541 atacaaacgt cgtatccctg aacggattgt tttctgttaa gttcaggttg tgagcataat
     6601 tcgccggagg gatattaaaa tgaatggaaa attgcaaagt tcggatgtaa aaaacgaaac
     6661 tccgtacaat attcccttat tgattaacga aaatgtgatc tccagcggaa tttctctgat
     6721 atcgctctgg catacctacg ccgacgagca ttatcgggtg atctggccgc gggacaagaa
     6781 gaaaccgctt atcgccaact catgggttgc ggtgtatacc gtacaaggat gtgggaaaat
     6841 tcttttaaag aatgacgaac aaataactct gcatggcaac tgtattatat ttttaaagcc
     6901 aatggatatt cactcttatc actgtgaagg tttggtctgg gaacagtatt ggatggaatt
     6961 tacccccacc agtatgatgg atattcccgt cggtcagcaa agcgttattt ataatggcga
     7021 agtttataat caggaactca ccgaagttgc tgagttaatt acttcaccag aagcaataaa
     7081 aaataatctg gcagtcgctt ttctgacgaa aattatttat cagtggattt gtcttatgca
     7141 cgcagtcggt aaaaaagatc cacaacggcg gcaaattgaa aaattaattg ccactttaca
     7201 tgccagtctg caacaacgct ggagcgtagc tgatatggct gccacgatcc cctgtagcga
     7261 ggcctggttg cgtcgtctgt ttttacgcta taccggcaag acgccgaaag aatattacct
     7321 cgatgcgcgt ctggatctgg cgctatcgct attaaagcaa caagggaact cggttggcga
     7381 agtcgctgat acgctcaact tcttcgactc ctttcatttc agcaaagcct ttaagcataa
     7441 atttggttat gcgccgtcag ctgtgctgaa gaatacggac aggtaataac cagaatggcg
     7501 agtgtgtaaa tgactcgatg atgttcagaa ataccaggtg gagtaccatt tacggcgact
     7561 ccacccggaa aagagttaac cgatactcca gaagagcacc gccagtaatt ggggggtgat
     7621 aatgcgcagg aacatcacca acggatagac agtggcgtaa gagagcgccg ccgcaccgct
     7681 cgttggatga agattattag caaatgccag cgccggagga tcggtcatgg agccagccag
     7741 catcccgcac atggtcaggt aattcatttt ggctaacatc cgcgccagaa tgccaacagt
     7801 aatcagcgga acggcggtga tcagggcacc ataaccaatc cagcttagcc cttcgccatc
     7861 caccagtgta tggataaaat cgccgccgga tttcaggcca accaccgaga gaaagagcac
     7921 aatgcctaac tcgcgtagcg ccaggttagc gcttggtggc ataaaccagt acagcttgcc
     7981 gatactgccg atacgcccga ggatcaacgc cataatcagc ggaccgcccg ccagcccgag
     8041 tttcaacgcc gccgggaacc caggcacaaa gacgggaata gaacctaaca atacgccaag
     8101 cccgatacca ataaacactg gcagcatctg aacctgttgc agtttttgct gcgcattccc
     8161 cagcacattg gcaacggcat caattgcgga cggacgcccc accaggttga ggatatcgcc
     8221 gaactgcagg ctgatatcgc tacttgcaac taattcaaca ccagcacggt tcaggcgcga
     8281 gataacaacg tcatagcgtt ctttaaagtg caggtcgcga atacgtttac cgagcacgtt
     8341 ttcattggtg accaccacac gctcgacgcg caagtcagtg cctttcgtag acagcgaggt
     8401 atcgacctcc tgaccaatca ccagttgcgc attatgtaaa tccgcaggct gccccaccag
     8461 atgcagcaaa tcgcccagtt gaataacggt ttctggcgaa ggcactttta gggtttcttc
     8521 tcgtttcaat cgtgagcaaa taactttatc gccattgaga atcggcacat ctttaatggc
     8581 taaattatgc aaattaggat tctcaactcg gatgttaatc gtcctgatta gcgcaccgcc
     8641 gttggttcgc gtggactcgt gctgctgggc ctcagtctca acattgacgc ggaaaataac
     8701 ccgcagcatc cacatagtga acaaaatgcc gcaaatccca aatggatacg ccatcgcata
     8761 gctcatcccc atttgatcga ccatcgccat cggtgtcccc aggtcgcgca agatctgctg
     8821 ccctgccccc agtgctggcg tattggttac cgcaccggag aaaatcccca gtactaccgg
     8881 cagtggaata tcaaacagtt tatgcaggat ggcggtaacc agccccccga tgatgacgat
     8941 cagaacagca aacaggttga ggcgtaatcc ggagacgcgc aatgaggcaa agaagcccgg
     9001 ccctacctga atcccgatgg tataaacgaa caggatcagg ccaaattcct gaataacatg
     9061 cagcatatcg ctacttaatg tcatccccgc ctgagaaaca aaatggccga cgatgatccc
     9121 accgaacagc acgccgccaa tgcccaatcc aacaccgcga aacttgacgt tgccgataaa
     9181 caacccgacg accgccacca aagccagaat actgaccgtt aatgctatat cactcatcgt
     9241 ccatttctct tgagataaca ttttaattgc tacggattat ggctgaatcg gtgaagcaga
     9301 tttgccttat agcgcacatt atcacgggaa agaacgtgcc gaaatatctt aaacaggcga
     9361 aaattaacgc attgttgaag aagaaagaca aaaaaagccc caccagaatg gcggggcaaa
     9421 gagaatagct agttagctat ttaacgcggg acgttcgctg atagcgatac gctgcgctgc
     9481 gatgggttca ggctcattac gaattaaatc aatatgcagt aaaccgttta cgaaggttgc
     9541 accagagact tccatatttt cagccagcgt aaagctcagg ctaaatggct gattcataag
     9601 cccttgatgc agccattttt tctcttcttt tggctgctcc ggcgtgcctt ttacgctcag
     9661 gcgcgtacct tccagttgaa tctctaaatc ttcctgacga aaacctgcca gcgcaagggt
     9721 aatgcggtag tggttatcgt cgcttttctc aatgttgtac ggcgggaagc tctggctttc
     9781 accggcgttt tgcagtgcgt tggccagttt gtcaaaaccg atccattgac gcatcagtgg
     9841 ggataaatcg aagttacgca tagtcatttc tccttctaag aagcgagtaa gtacctgcaa
     9901 atccgaagat tcgcatatgc tccctgacgg cgagcatgga gatgtcaggc cgcgcaaggc
     9961 ggccttaggg aattagttga tttcgatacg gcgcggtttt ttcgcttccg gaatcacgcg
    10021 ttcgagatcg atatacagca aaccatttac caggttagca ccacgaacat gaatgttctc
    10081 agctaactgg aatttgcgtt caaagttgcg ttcagcgatg ccctggtaca gataggtgcg
    10141 ctctttttgt tcgtcggcgt gagcaccttt caccaccagc agattatcct gggcggtaat
    10201 ttccagttcg ctctcagcaa aaccagccac agcgatagca atgcggtaat ggttttcgtc
    10261 taccagttca acgttatacg gagggtagcc gccattactc tggctctggt tgttttctaa
    10321 gtggttaaac aaacggtcaa atccaatagc agaacggtaa agcggggata aatcaaagtt
    10381 acgcataatc aatagctcct gaaatcagcg agaatctaag accttccaca atggacaggt
    10441 caggtagcca gaacacccat caggcgcgtc ctcatcggct acgatgtaaa aatgggtctg
    10501 gaaatgaact tttcaagcct tatcagcgga ctttttttgc agtttatggt ctattgcata
    10561 gactgagggg gcagcacgaa acgttaaagt gcgatagccg ccacagtgcg acgtaatgat
    10621 gatgttattt tcagcaaggg atcgctataa ctcatcaggc caacacccac gatcaacaga
    10681 tgaatttatt atgataagaa atgtgttgtt agcgttcatg atatgcagcg gaatgacatt
    10741 acttggagga tgctccagcg tgatgtccca taccgggggg aaagaaggaa cgtatcccgg
    10801 cacccgcgcc agcgctacca tgattggcga cgatgaaacc aactggggca ctaagtcgct
    10861 ggcgattctc gatatgccgt ttaccgctgt gatggacacg cttttactgc cgtgggacgt
    10921 gttccgcaaa gacagctcgg tgcgctcacg cgttgaaaaa agcgaagcta acgcgcaagc
    10981 caccaatgcg gttattccac ccgccagaat gcctgataac tgatttagcg atcagtttcg
    11041 gtgatccaca gctggccgcc ttccatccac gccagccact gaccatccgg cgagaagacc
    11101 acggcgtccg cagaaggtgg attggcgtgg ttttcggtta aatactcaac ctcgccgctt
    11161 tgcgcatggg cgcaagcaat tcgattatcc agcacaaagc ccaaccattc tcctgatgga
    11221 tgccagttaa atgcagactg aatatccgtt ttgttatggg ttaactggcg cggctcgccg
    11281 ccctgtggcg agataagcca cagttgcaca atgccgttat catcacgcat taaaaacgcg
    11341 atttgcgtac cctgcggatt acagcgcacc cagtggcgcg ggacgttgac taaccccgga
    11401 taagcccgat ggtgggtaaa ggttaaacgt cgctgcacga cgccacgcgg tggcgcgggc
    11461 agggttgttt ccgttccact taacggcgca tcaccagctg ctttccagcc agcttcatct
    11521 tgcggtaact caacgataaa tagctccggc actttctcgc cctttggcga aagtgtgtcg
    11581 ccaataaacg ccagcgcgtg atttcctacc catccttctt cataagcacg attgatttca
    11641 ttactgccag gctgcggcgt gggcgttgtt ttactcacca gcacgcacca gtggctaccg
    11701 ctgtattcac gcggatgctg cttttgtacg ttgaccgggc caaacggcgc agcaacacct
    11761 acgtttcgca aatccagcgt cgaatcgagt tgatgcatta catggtcgtt ataggtaaag
    11821 ctcacccttt ctccgttcgg gctaaagaca tgcacatggc tgccgccgcg cagcgctcct
    11881 ggggtgtacg gcgcggtaat atccattgca tcgagattgc tcaccttgcc gccttcagca
    11941 atcactccgc gccgatgatg gaagtcgtaa taccatgttt catcaggatt ttccgggccg
    12001 tggatgaaaa catatttctc tgacttcgga tgaacggtca ccacgccgac gtacgcgccc
    12061 tgtgacgcgc gatagataac ctcgacctcg ccggtatgga tattcacacg ctcaatggtc
    12121 tcgccggtaa acgacgcccc tgaaggacgc acgtcaaata ccagccactg gctatcgggc
    12181 gtccaggtat tggtattggt gagcaggtga ttacggggag caaaggttat ctgtttcatg
    12241 gcgcggtcct gatacaaaac gggtcccgcc atcatacttc acaaggactt cactttcaga
    12301 caatagtctt ttgccatcgt gatgaatgtg agtgaaaaga tggaacattt cgacgtggcg
    12361 attattggcc tcggcccggc agggtcggcg ttggcgcgaa agttagcagg caaaatgcag
    12421 gtgatcgcgc tggataaaaa gcaccagcat ggtactgaag gtttcagcaa accttgtggc
    12481 ggtctgctgg caccggacgc gcagcgatct tttattcgcg atggactgac gcttcctgtc
    12541 gatgtcatcg ccaacccgca gattttcagc gtcaaaaccg tcgacgtcgc cgcatcgctc
    12601 acgcgtaact accagcgaag ctatatcaat attaatcgcc atgctttcga cttgtggatg
    12661 aaatcgctga tccccgccag cgttgaggtt taccacgaca gcctgtgtcg aaaaatctgg
    12721 cgtgaggatg ataaatggca tgtcattttt cgcgcagacg gctgggagca gcatattacc
    12781 gcccgctatc tggtcggtgc cgatagtgcc aactcgatgg tgcggcgaca tctctacccg
    12841 gatcatcaaa ttcgtaaata tgtcgctatc cagcagtggt tcgcagagaa acatccggtg
    12901 ccgttctact cctgcatctt tgataatgcg ataactgact gttattcatg gagtatcagc
    12961 aaagacggtt attttatctt tggcggtgcc tatcccatga aagacggtca gacgcgtttc
    13021 acgatgctga aagagaaaat gagcgccttt cagttccagt ttggtaaggc ggtgaaaagc
    13081 gaaaaatgca cggtgctgtt tccctcacgc tggcaggatt ttgtctgcgg taaggacaat
    13141 gcctttctga ttggtgaagc ggcgggattt atcagcgcca gctcgctgga ggggattagc
    13201 tatgcgctgg atagcgcaga gattctgcgt tcggtgttac tgaagcagcc ggagaagagc
    13261 aacgccgcct actggcgcgc cacccgcaaa ctgcgtttaa aactcttcgg caagatagta
    13321 aaaagccgat gcctgaccgc accggcttta agaaagtgga ttatgcgcag tggtgtggcg
    13381 catattccac agttgaaaga ttagccaacg cgcttcacat cgcccaccag caggatgtaa
    13441 gagagcgcgc caatcaacgc gacggcggag atataaacca gtgcaggtgc gaaaccgtaa
    13501 ccctgcgcca ggtaccccac caccagcgga acggtgatgc cgcccagacc accggcgaag
    13561 ttaaacacgc cgccggttaa accaatcagg cgcatcggtg ccagagaaga gaccagcgac
    13621 caggtaatcg aagcaaaacc gttgccgaag aatgccagcg ccatcaggca cataatcatc
    13681 atcggatcgt tggtgtagtt agcgcccata atgcaggtgg agatcagcaa gccgcagata
    13741 atcggcgttt tacgcgcaaa gcccagtgaa aagcccttac gtaccagcag atccgcgacc
    13801 cagccagaga gcaggacgcc gacaaacgcc gcgaggaatg gcaccgtggt cataaagcct
    13861 gctttcagcg ccgtgattcc tttttcctgg gttaaatagt tcgggaacca agttaagaaa
    13921 aaccacagtg tagaagccac cgcaaattgc ccaagataga cgccgatcag tttacgatgg
    13981 aacaccagtt tccagtcttt ggctgttaac ggctgacgcg cctctttttt caccggcgca
    14041 tcaccatcca ccagaccgcc gccatcacga atgtaatcca gttcagcttt gctgatgcct
    14101 ttagtcaggc gcggcggctg ataaacctta aaccaaatca gcgaccagat aatgccgata
    14161 ccaccggtga caatgaacac ccagtgccag ctcaacattt cctgaatcca gatcagcagc
    14221 ggagtcaaaa acgccagacc gacaaactga ccagacgtat aaaaaccaac ggcggaagcg
    14281 cgttcatgtt ccgggaacca gctggtcacc atccggttat tggtcgggaa agcgggcgct
    14341 tcgaaaatac cggttattgc gcgcagacca attaatgaca tcaatcccgt ggcaaagccc
    14401 tggaataaag tcgccactga ccagccaaat atcgcaataa aataagtcac gcgtgaacct
    14461 acgcgatcta aaaaccaacc gccggggatc tgacatagtg tataaagcca ggcgaaggcc
    14521 gaaaatacat agcccatttc cgctttggta ataccgaact cttcctgaat atgggcggaa
    14581 gccacggcca ggttagcgcg gtcaacataa caaatgacca ccgtaataaa gatcatcacc
    14641 agcgtcagat aacgccgacg ccccggcttt gctgcattaa cgggaatatc catagcgagc
    14701 tttctccaga ttttgggcat agcgaagccg ctcaccatgc cctgtaattt acagagggtt
    14761 atatttttgt attgctgttt tagtgcccga tgagaggctt acgtggcagg aattaccact
    14821 ctgctacgct gttatcttca tgacgccaga gcggattacg ccagtccggg gcatttttac
    14881 tgaactcaat aactttagct tcgtcgattt ccacacctaa gcccggtttc gttaatggtt
    14941 taaagaaacc gccgaccatg ctgaagtctt ctttgttttt cacaaagtcg agtaactccg
    15001 cgcctttgtt gtaatgaatg cccatacttt gttcctgaag tacggcgtta taggaaacaa
    15061 agtcgatatg caggcaagcc gccagtgcaa tcggtccgag cggacagtgc ggcgcaaggg
    15121 tcacgtcata ggcttctgcc attccggcaa ttttgtagca ttcggtaata ccgcccgcgt
    15181 gggagagatc tggttgcaga atcgaaatac cacccgcctc cagcacacgt ttaaaatcga
    15241 aacgtgagaa catgcgttca cccgccgcca gtggaatatg cgtttgtgcc gccagtttcg
    15301 ggtagtattc ggcttgttct gccagcaccg gctcttcaat aaacagcggg cgatacggct
    15361 ccagttcttt aatcagcact ttcgccatcg gcgcgctgac gcgaccgtgg aaatcaagac
    15421 caaactcaat ctgattgcca aaagcttcac gaatttgtgc cacggtgttg actgccgcat
    15481 ctaccgcgcg ggagttatca attagcccca gttcttcaca accgttcagt ttgaaggtat
    15541 cgaagccgat ttcgcgcagg gttttaatgc cgtcgataac atccgccgga cgatcgccgc
    15601 cgacccaact gtaggcttta attttgtcgc gaaccaggcc gcccatcagt tgccagaccg
    15661 gcgcattcag cactttgcct ttgatatccc ataacgcctg gtcgatcccg gcgatggcgc
    15721 tcatcaggat tggaccgcca cgataaaatc cggcgcgata catcacttgc cataagtcat
    15781 tgatgcgcga tggatcctga ccaatcaaat agtcacccag ctcgtgaact gccgcttcca
    15841 ccgtgcgggc gcggccttca atcactggct cgccccagcc gaccacgcct tcatcggttt
    15901 caattttcag gaacatccag cggggaggta aacgatacgt ggtaattttg gtgattttca
    15961 ttgcactgcc tctcgatacg ccttaacaaa tgctgctgcc tgctgcgcgg tgcgttctac
    16021 ggactgcccg gcgcgataga gatcgctgcc taagcccgcc cctgcacaac ctgcgtctat
    16081 ccactgcgcc aggttttctg gcgtcacgcc gccaacggca aagactgcga tgtccggtgg
    16141 taataccgct tttaacgctt tgatgtattg cggaccaaaa gccgatgacg gaaatatttt
    16201 cagcgcctga gcgcccgcgt cgagcgcggt aaaggcttcg gtcgccgtcg cgcagccggg
    16261 gcaaacggtc atgccgtagc ccaccgcacg gcggatcact tcactatgga tattgggcgt
    16321 aacgatgagc tggcagctca tcctggcgag tgcatcgacc tgttcaggtt tcagtaccgt
    16381 acctgcgcca atcaacgcct tatcgccata cgcatcaacg atggcgggaa tgcttttctc
    16441 ccattgaggg gaattcagcg ggatttcaac cgcgtcgaac ccggcgtcaa tcaccgcgcc
    16501 aacatgcgcc agcgcctcgt cgggcgtaat gccgcgcaaa atggcgatca gcgggagttt
    16561 agtttgccac tgcatgagcg atgctcctta taccagcctg aaatgccgtg tcgcccgcca
    16621 ccgccgtcac gtcgcaaccc atcgcctgaa aggcttgctg gtagcgcgcg gtcagcgatg
    16681 ttccggcgac aagggtgatg gcgtgttgat gggtcacata gtcgcgcata ctggcaacct
    16741 ctgcgccaat caacaaacca gagagaaatt cgctgacctg ttcgcgggga agtgttccca
    16801 gcacatgcga ggcgcgaact tcaaaaagct gcggcaatat ggcgggagca ttaaagccac
    16861 gctcaagacc agccgcgaag gcatcggcag agttttcctg cggcggcaaa cctgcgccaa
    16921 tcaatgagtg atttaacagt aaatgatgta attcaccggt catcacggtg cgaaaatcgt
    16981 tgatttgctg gctatcggcc tgcacccatt tgcaatgggt accgggcata acataaagag
    17041 aggaaggagc gagagcacgc gcgccgatca attgtgtttc ttcgccgcgc atcacattgt
    17101 ggttatcgtc atgagagacg cataatccgg gaataatcca gatattgtcg ccaaccgacg
    17161 ttaattgatc gccaatagac gaaaaacggg caggaacaga taaatacggt gcaactttcc
    17221 agccgacatt gctgccgacc attcctgcca tcaccactgg cgtattctct tcacgccagt
    17281 cggtcgtgac ttctgctaac accgcagccg gagattttcc gttcaggcgc gtgacgcctg
    17341 cttctgattg cctgctgtcc aggcagtggt cgccctgata aagccaggcg cgcagattgg
    17401 tcgatcccca gtcaattgcg atgtagcgag ctgtcatgtg atttccttta accttcgtgt
    17461 cgagctggcg atcatggtaa gcgccgcctg ctctgccgca tcgccgtcct gatgccgtat
    17521 tgcatcgaac agcgccttat gttcctggag cgtttgcggc atgttggcct catcgcccat
    17581 ccaggttcgt tcaaaaaccg cccgctgcag cgaactgatc gcaatgctaa gttgctgtaa
    17641 caccgggtta tgcaccgact gcagcaccgc ctcgtggtag cgaatatccg cttcgttaaa
    17701 cgcttcgcgg tcctgattgt tggcaatcat ctcgttcagc gccgattcaa tctgcgccag
    17761 atcgctggag gtcgcgcgct ctgctgccca acgggcaata gccggctcta ccaggtttcg
    17821 cacttcgctc attgcactga taagccgtgg gtcatagtcg ttttccagca cccattgcag
    17881 tacgtcagtg tcgaggtaat tccactggtt acgcggagcc acaaacgccc cgcgataacg
    17941 tttcatttca atcagccgct tcgccatcag cgaacgaaac acctcacgga tgatgttgcg
    18001 cgaggttgca aactcttcac agagctccgc ctcagccgga agtggagagc ctggtacgta
    18061 tttgccgtga acgatctgtt tacccagcgt aataacgatg cgatcggttt tattgagagt
    18121 catggagagt ccttgtgctc tgtatgttct tctctacttt accccgatcg atgcataacg
    18181 cggcaacttt gtagtaccag cgtgatgacg ttcgcgtttg ctgtgcgcct aatgtagtac
    18241 aaacttatat tgttgtacta caatttagat cacaaaaaga acaatacata aaaaatgaca
    18301 tgcgtcgggc agaaatctga aaagggatgt caggcgctaa acaggaggga aagaagagta
    18361 tgctttcaac ggcttagcta ctcgtttaaa ggattaatca tgaagttgaa ttttaaggga
    18421 ttttttaagg ctgccggttt gtgttctttg gcatttgcgt tgaccggatg tatcacctgg
    18481 ggattggttt ccaatactgc aagccgtcca catgccgagc aatggcggtc agacacgatt
    18541 aagggtttat cgctggctga agacagtaac ggtacgaaag gctatgtttt tgttggtgaa
    18601 tcgttggact atctgttgac caccggcggc gatgaggtgg ttaaaatgct gaacgatccg
    18661 gcaatccatg gtgaaagaat caccgtttct gataacgcga aatttatttt aagcagcagt
    18721 aataagaatt tctctggtgc tattacgctc tattacgact ggaacaacga agaagataag
    18781 gcactggcaa cacaatatgg ttttatttgt gatacccggc gttgtacatg gatgctggac
    18841 ggtttgaaag gctcaatcca ccagaaaaat aaaaaagcgg attactcaaa cgtgatggtt
    18901 ttccatcaac cctttacggt gggattttat gagtacaaag caacagatgg cgtcccgcgc
    18961 ggcctcgtta atgcgctgct gccagtcact cttacgctgg atatagtaac gtcaccactc
    19021 cagttcctga ttttatgcac gaccagaaat tgttaatcgc tgcgggcaaa cgcattcatg
    19081 ccgtttgccc attcactaat taattcagca catacttctc aatagcaaat gccacgccat
    19141 cttcaaggtt ggatttggtg acaaagttcg ccacttcttt caccgaagga atagcgttat
    19201 ccatcgccac accgacgcct gcatattcaa tcattgcgat gtcgttttcc tgatcgccaa
    19261 tcgccatgat ttcttccggt ttaataccta acacgtcggc cagtgatttc acacccgtac
    19321 ctttgttaac gcgtttatcg aggatttcga ggaagtacgg cgcacttttc agcacggtgt
    19381 atttttcttt cacttcctgc ggaatacgcg cgatggcctg gtcgaggatg gcaggttcat
    19441 caatcatcat cactttcagg aactgggtat tggggtccat tttctccgct tcgcagaaca
    19501 ccagcggaat ggtggcaacg aaagattcat gcaccgtgta gtagctgata tcacgattgg
    19561 cggtgtacag cgtggtgcgg tccagggcgt ggaaatgaga accgacttcg cgagagagtt
    19621 tttccaggaa acggtagtcg tcatagctga gagtggtttg cgccacggtg ctaccatcag
    19681 cggccttctg taccagcgcg ccgttataag taatgcagta gtcgcccggc tgttccatat
    19741 gcagctcttt caggtaattg tgcacacctg cataagggcg acccgtcgtt agcacgacat
    19801 tcacgccacg ggcgcgagct gcggcaatcg catttttaac ggcgggtgaa atggtgtgat
    19861 cgggcagcag aagggtgcca tccatatcga tagcaatgag tttgatagcc atgagttccc
    19921 caggtagatt ggttcctgac ccatgctaac gcgattccgc tcaaaaatca gtacaacacc
    19981 cgagggaaaa gggggatgca acgcgcgttg cgtgctcccc ttttgcttag cggaagagtt
    20041 tccctttcag cagttccatg cctgcggaaa gcagatcgtt attggcttgt ggtgacactt
    20101 caccttgcgg tgagagtgca tcaataatct tcggcaattg ttctgccagt aaactggaag
    20161 ctgtactggt atccacgccc agtttttgcc cgagatcgga caccgcattt gtgccgagcg
    20221 ccgattccag ttgctcgccg ctaactggtt ggttgccctg ttgattactc agccaggttg
    20281 agagaatggc acctaaaccg ccactttgca gtttttccag cagcacctga atgccgccct
    20341 gctcctcaac ccaacttaaa atcgcctgat atttccccgc atcgcctttc agaaaggcgc
    20401 cgacaacgtc atcaaaaagg cccatgataa tcacctgtaa agcgttacgt gttgactcaa
    20461 aaagtatagt tctgtgggat gataattgcg gattgcagga ataaaaaggg cggagatgat
    20521 ctccgccctt ttcttatagc ttcttgccgg atgcggcgtg aacgccttat ccggcctaca
    20581 aaaacatgca aattcaatat attgcaggag tctcgtaggc ctgataagcg tagcgcatca
    20641 ggcacgctcg cattgttaac gccattaaat atcaatattc gccgctttca gagcgttctc
    20701 ttcaataaac gcacggcgcg gttcaacggc gtcgcccata agcgtagtga acaactggtc
    20761 ggcagcaatc gcatctttaa cggtaacgcg cagcatacga cggctttccg ggtccatggt
    20821 ggtttcccac agctgttccg ggttcatctc gcccagacct ttataacgct ggatggagag
    20881 gccgcgacgg gactctttca ccagccagtc cagcgcctgc tcgaagctgg ctaccggctg
    20941 acgacgttcg ccacgttcga taaatgcatc ttcttccagc aagccacgca gtttctcacc
    21001 cagcgtgcag atacgacgat attcgccgcc agtgataaat tcgtgatcca gcggatagtc
    21061 agtatccaca ccgtgggtac gcacgcgaac aatcggctcg aacaggtttt gttcggcatt
    21121 ggtgtggaca tcaaacttcc actggctgcc gtgctgttct ttgtcgttca gttcgctgac
    21181 cagcgcgttc acccagcggg taacggtctg ctcatcagag aggtcggctt ccgtcagcgt
    21241 cggctgatag ataagctctt tcagcattgc tttcggataa cgacgctcca tgcggttgat
    21301 cattttctgc gtcgcgttgt actcagacac cagtttctct aacgcttcgc cagccaatgc
    21361 cggtgcactg gcgttggtgt gcagcgttgc gccatccagc gcgatggaga tctggtactg
    21421 atccatcgct tcgtcgtctt taatgtactg ttcctgcttg cctttcttca ctttgtacag
    21481 cggcggctga gcgatgtaga cgtgaccgcg ttcaacgatt tccggcatct gacgatagaa
    21541 gaaggtcaac agcagcgtac gaatgtgcga gccgtcgacg tccgcatcgg tcatgatgat
    21601 gatgctgtga taacgcagtt tgtccgggtt gtactcgtca cgaccgatac cacaaccaag
    21661 cgcggtgata agcgtcgcca cttcctggga agagagcatc ttatcgaagc gcgctttctc
    21721 gacgttgagg attttaccct tcagcggcag aatcgcctgg ttcttgcggt tacgcccctg
    21781 cttcgcagag ccgcccgcgg agtccccttc caccaggtac agttcagaga gcgccggatc
    21841 gcgttcctgg cagtctgcca gtttgcccgg caggcccgct aagtcgagcg cacctttacg
    21901 gcgggtcatt tcacgcgcgc gacgcgccgc ttcacgggca cgggcagcat cgataatttt
    21961 gccaaccacg attttcgcgt cggttgggtt ttccagcagg tattctgcca gcagttcgtt
    22021 catctgctgc tcaaccgccg atttcacctc agaagaaacc agtttgtctt tggtctggga
    22081 ggagaatttc gggtccggca ctttcacgga aacgaccgca atcaggcctt cacgcgcatc
    22141 gtcaccggtg gcgctgactt tggctttttt gctgtagcct tctttgtcca tgtaggcgtt
    22201 cagggtacgg gtcatcgccg cacggaagcc tgccaggtga gtaccgccgt cacgctgcgg
    22261 aatgttgttg gtaaagcagt agatgttttc ctggaagcca tcgttccact gcaacgccac
    22321 ttcgacgcca ataccgtctt tctcggtgga gaagtagaag atattcgggt ggatcggcgt
    22381 tttgttcttg ttcagatatt caacgaacgc cttgatgccg ccttcatagt ggaagtggtc
    22441 ttctttgccg tcgcgcttgt cgcgcagacg aatggaaacg ccggagttga ggaacgacaa
    22501 ctcacgcaga cgtttcgcca gaatttcata ttcgaactcg gtcacattgg tgaaggtttc
    22561 gaggcttggc cagaaacgca ccatggtgcc ggttttttca gtctcgccgg taaccgccag
    22621 cggggcctgc ggtacaccgt gttcgtagat ctgacggtga attttaccct cgcgctggat
    22681 aaccagctcc agtttttgcg acagggcgtt tactaccgaa acaccaacgc cgtgcagacc
    22741 gccggacact ttataggagt tatcgtcaaa tttaccgcct gcgtgcagaa cggtcatgat
    22801 cacttccgcc gccgatacgc cctcttccgg gtgaataccg gtcggaatgc cgcgcccgtc
    22861 atcctgtaca gagacagagt tatcggcgtg aatggtgacg ataatttctt tacagtgacc
    22921 cgcgagcgct tcgtcgatag cgttatctac cacctcgaat accatgtggt gcagaccggt
    22981 gccgtcatcc gtgtcgccga tatacatacc cgggcgctta cgcaccgcat ccagcccttt
    23041 caggactttg atactggagg agtcataaga attcgacatc aacgtttctc gctcatttat
    23101 acttgggtta atccgttatt ttaccctttt ccacggtaaa catcttcgaa ttttcgtccg
    23161 acatgtctat aacgtgttca gcactgatcg cgctgacaaa gacctgtgat tgcgtcgctt
    23221 ttaagcggct ggcaagcagc ccgcgacgct catcatcaag ctcagaggca aaatcatcta
    23281 tcaggtagag acaccgccgc ccgctttcac gggtgaggaa ctctccttgc gccagacgta
    23341 aggcgcacat caacagctta agctgcccac gcgataaggt atcttccacc ggcgcaccgt
    23401 cggcgcgaat gcgtaagtcc gctttatgcg ggccgtgcgc ggtgtaggtt agctggcgat
    23461 cgcgttcaaa attacgttcc agcacctcag cgtactctgt ctctttctcc cagccgcgct
    23521 ggaaagagaa agtcagagaa aactcaggga gaaattgctt acaggtatcg gccatatcag
    23581 ccgcgatacc ggcgctatac tccgcgcgcc aggtgcttat ttgctccgcc agcggaatca
    23641 gttctttatc ccacgggcgt agctgttcgt aacgtgtcac ctggcgcagc gccgcattgc
    23701 gctgcttgag caatcgcttg agattgctcc aggcggtgaa aaatccgggt tcgttgtgaa
    23761 agcatcccca gtcgaggaat gctcttctgt atttggggcc gccgttgagt aaagtaaacc
    23821 cttctggcgt tatcaactgc attggcatca ggtgcgccag ttccgcgacc ttatgcccgt
    23881 ctgtaccgtc gatgcggact ttgctgtcgc cctgtttgtc tttggttaag ccaatcgccg
    23941 tctcgcgctc ttcgccctgt aatcgcccgt ggagaacaaa cgcctcctgc tcgtggcgaa
    24001 tgacgcgacc aatctgcaaa ctgcgaaacg cccgaccatg gccgagcgta tagatggctt
    24061 ccagcacgct ggttttgcca ctaccgttgg cacctaccag aaagttaaag ccgggagata
    24121 aagcgagatc cgcggtttcg atattgcgga aatcgcggat caacaagcgg gtgagggaca
    24181 ttacagtctc attggcatga caacataggc agccgactgt gatgcggcat cttcaatctg
    24241 cacgctggaa accgaatcgg tcagcatcat gcggacgttt tcgcatttca gtgcgttcag
    24301 aacatccagc acatagctga cgttgaagcc gatttccatc tccgcaccgc tataggtaac
    24361 gtcgaggatc tcttccgctt cttcctgttc tgggttgttg gcggtgattt tcagctggtt
    24421 ttcgctgaca tacagacgca cgccgcggaa tttctcgtta gagagaattg ccgcacgggc
    24481 aaacgcctgc ttgagcagat cacagccagc ttccagatgc ttgtccgggt tcttcggcag
    24541 aacgcgacgg taatccggga agcgaccatc caccagtttc gaggtgaaga taaagtcgcc
    24601 aacgtgggcg cgaatgttgt tgctgccaat ctgcacgcgc agcggattgt cgccgccgtc
    24661 gagcatacgc atcagttcaa tcacgccttt acgcggcacg atcaccgaat ggcttggcaa
    24721 agattgacca attggcattg aacagactgc cagacggtgg ccgtcggtcg ccacggtgcg
    24781 cagttcttca ccttcggttt caaacagcat accgtttaag taatagcgaa cgtcctgatg
    24841 cgccatagaa aactgggtcg cttcaatcag acgcttcatc gtcgcctgcg gcagggtaaa
    24901 ttcgacttcg ctctgccagt catcgaggtt cgggaaatct gccgctggca gggtagacag
    24961 cgaaaaacgg ctacgcccgg agcgcaccag catccgctca ccttccagct gcacggcaat
    25021 ttccgcgcct tcaggcagac cacggcagat atcaaagaat ttgcgcgccg gaacggtcgt
    25081 cgcccctggt tcgtgcggct gaaccagcgc aacgcgtgcc accatttcca tctcgagatc
    25141 agtaccggtc agcgacaacg taccgtcagc aacctgtaac agcagattac cgagaatcgg
    25201 tagcgtagga cgaccaccta acggaccgct cacctgttgt agcggtttta ataaatgctc
    25261 acgttctacg gtaaatttca taggtttacg atgacaatgt tctgattaaa ttagaaaaat
    25321 cttctttgat atcgtggctc tcttcacgca actgctcgat cttccggcag gcatgaagca
    25381 ccgtcgtgtg gtcacgacca ccaaaggcat cgccaatctc cggaagactg tggttagtca
    25441 gctctttcgc cagcgccatc gccatttggc gcggacgggc caccgagcgg gatcgacgct
    25501 tggaaaggag atccgcgact ttgatcttgt agtactccgc caccgtcttc tgaatattgt
    25561 cgatggtgac cagtttttcc tgcaatgcca gcaagtcgcg cagcgcctca cgcacgaagt
    25621 cgatggtgat cgcccgtccg gtaaagttgg cattggcaat gacgcggttc agcgcgcctt
    25681 ccaactcacg tacgttagat cgtagacgct tggcgataaa aaacgccact tcgcccggca
    25741 aacgaatgtc gttttcgtcg gcctttttca tcaggatcgc cacgcgggtt tccagctctg
    25801 gcggttcgat cgccacagtc agtccccagc cgaagcggga tttcaaacga tcctcaacgc
    25861 cgttgatctc tttcggatag cgatcggagg tgagaatgat ctgttgatta ccttccagca
    25921 gggcgttgaa ggtgtggaaa aactcttcct gagatcgttc tttattagca aaaaactgaa
    25981 tatcgtcgat cagcagtgca tctacggaac ggtagtagcg tttaaactct tcgatcgcgt
    26041 tgttttgcag ggctttaacc atgtcctgaa caaagcgctc ggagtgcata taaaccactt
    26101 tggcattcgg cttgcgcgcc ataatgccgt tacccaccgc atgcagcagg tgagttttac
    26161 ccaaacccgt gccgccataa aggaacaacg ggttataagc accgccaggg ttatctgcca
    26221 cctggcgagc cgccgcgcgc gccagttggt tagatttacc ttcaacgaag ttatcaaacg
    26281 tgtgtttgac gtttacgtta gaacgatagg tcggttctgc cggagccggg acgttatccc
    26341 aacccgagcg cgtagaaggc gcagcacgtt gcggctgcgt ttgcgccacc tgtgcagggg
    26401 ccgcgacgtt gctcgtcact gccgcttgtg gcgtttgcgt caccggtttg gtgccgactt
    26461 caaaacgcag ctgtggggca tccgctccgc agaaactggt tagcagtcca ttgatattat
    26521 taaggtactt gtcccgtacc caatcgagga caaaacggtt tggcgcgtac agggccagcg
    26581 tgttatcgct cagttccgcc tgcaatgggc gtatccacat actgaattct gtggctggta
    26641 actcatcctg caatcgggca agacactgct gccaaagcga aagtgacacg gcggactcca
    26701 ctcgaacaaa agtcgataat gactaaggct gaaacattca tgattgttga cgtacgtcga
    26761 aaagaccctg catgagggtg acgcacgaac cgctgtctgc ggttatatgc ccgatcaaga
    26821 tcctgcaaaa cgatcgggac cgcggatcat agcctaaact gcgcaagaga tcttctgttt
    26881 ctcacagatt ttcccgattt atccacagga ctttccagaa ctcgctaagt gtaaacgatc
    26941 ctgccgcggg ccgggcacga tttacgccgc atattggaaa atttaatgac catagacaaa
    27001 aattggctta atcgatctaa taaagatcca ggacgatcct tgcgctttac ccatcagccc
    27061 gtataatcct ccacccggcg cgccatgctg gttttcactg gtgtgaggcc gtacattttc
    27121 cctgcgaaaa ggtgcggaaa agcgcggtaa ataaggaaag agaattgact ccggagtgta
    27181 caattattac aatccggcct ctttaatcac ccatggcttc ggtgtccatc gtttcatttt
    27241 tcggcggata tccaataaag ccattgaatt tattcaagtt taggtagaaa tcgccatgaa
    27301 acgcactttt caaccgtctg tactgaagcg caaccgttct cacggcttcc gtgctcgtat
    27361 ggctactaaa aatggtcgtc aggttctggc acgtcgtcgt gctaaaggcc gcgctcgtct
    27421 gaccgtttct aagtaataaa gctaacccct gagtggttaa gctcgcattt cccagggagt
    27481 tacgcttgtt aactcccagt caattcacat tcgtcttcca gcagccacaa cgggctggca
    27541 cgccgcaaat taccattctc ggccgcctga attcgctggg gcatccccgt atcggtctta
    27601 cagtcgccaa gaaaaacgtt cgacgcgccc atgaacgcaa tcggattaaa cgtctgacgc
    27661 gtgaaagctt ccgtctgcgc caacatgaac tcccggctat ggatttcgtg gtggtggcga
    27721 aaaaaggggt tgccgacctc gataaccgtg ctctctcgga agcgttggaa aaattatggc
    27781 gccgccactg tcgcctggct cgcgggtcct gatagccctc attcgggtct atcaacgcct
    27841 gattagtccg ctactcgggc cgcattgtcg tttcactcca acctgttcaa gctacggaat
    27901 tgaggcattg cgcaggtttg gagtgataaa aggcagttgg ttgacggtga aacgcgtatt
    27961 aaaatgccac cctttacacc ctggtggtga cgatcccgtc ccgcccggac catttgatac
    28021 cagagaacac taacgatgga ttcgcaacgc aatcttttag tcatcgcttt gctgttcgtg
    28081 tctttcatga tctggcaagc ctgggagcag gataaaaacc cgcaacctca ggcccaacag
    28141 accacgcaga caacgaccac cgcagcgggt agcgccgccg accagggcgt accggccagt
    28201 ggccagggga aactgatctc ggttaagacc gacgtgcttg atctgaccat caacacccgt
    28261 ggtggtgatg ttgagcaagc tctgctgcct gcttacccga aagagctgaa ctctacccag
    28321 ccgttccagc tgctggaaac ttcaccgcag tttatttatc aggcacagag cggtctgacc
    28381 ggtcgtgatg gcccggataa cccggctaac ggcccgcgtc cgctgtataa cgttgaaaag
    28441 gacgcttatg tgctggctga aggtcaaaac gaactgcagg tgccgatgac gtataccgac
    28501 gcggcaggca acacgtttac caaaacgttt gtcctgaaac gtggtgatta cgctgtcaac
    28561 gtcaactaca acgtgcagaa cgctggcgag aaaccgctgg aaatctcctc gtttggtcag
    28621 ttgaagcaat ccatcactct gccaccgcat ctcgataccg gaagcagcaa cttcgcactg
    28681 cacaccttcc gtggcgcggc gtactccacg cctgacgaga agtacgagaa atacaagttc
    28741 gataccattg ccgataacga aaacctgaac atctcttcga aaggtggttg ggtggcgatg
    28801 ctgcaacagt atttcgcgac ggcatggatc ccgcataacg acggtaccaa caacttctat
    28861 accgctaatc tgggtaacgg catcgccgct atcggctata aatctcagcc ggtactggtt
    28921 cagcctggtc agactggcgc gatgaacagc accctgtggg ttggcccgga aatccaggac
    28981 aaaatggcag ctgttgctcc gcacctggat ctgaccgttg attacggttg gttgtggttc
    29041 atttctcagc cgctgttcaa actgctgaaa tggatccata gctttgtggg taactggggc
    29101 ttctccatta tcatcatcac ctttatcgtt cgtggcatca tgtacccgct gaccaaagcg
    29161 cagtacacct ccatggcgaa gatgcgtatg ctgcagccga agattcaggc aatgcgtgag
    29221 cgtctgggcg atgacaaaca gcgtatcagc caggaaatga tggcgctgta caaagctgag
    29281 aaggttaacc cgctgggcgg ctgcttcccg ctgctgatcc agatgccaat cttcctggcg
    29341 ttgtactaca tgctgatggg ttccgttgaa ctgcgtcagg caccgtttgc actgtggatc
    29401 cacgacctgt cggcacagga cccgtactac atcctgccga tcctgatggg cgtaacgatg
    29461 ttcttcattc agaagatgtc gccgaccact gtgaccgacc cgatgcagca gaagatcatg
    29521 acctttatgc cggtcatctt caccgtgttc ttcctgtggt tcccgtcagg tctggtgctg
    29581 tactatatcg tcagcaacct ggtaaccatt atccagcagc agctgattta ccgtggtctg
    29641 gaaaaacgtg gcctgcatag ccgcgagaag aaaaaatcct gattcggtga gttttcgcta
    29701 aaataagggc ggtcagttga ccgccttttt tcttttcgta gggcggataa gcaccgcaca
    29761 tccgccacac aaagcaacag gaacatcatg agcgataatg acactatcgt agcccaggcc
    29821 acgcctccgg gacgtggcgg cgttggcatc ctgcgcatct ccggcttcaa agcccgtgaa
    29881 gttgccgaaa ccgtgctggg taaactgcct aagccgcgct acgccgatta tcttccgttt
    29941 aaagacgccg acggcagcgt gctcgatcag gggattgcgc tatggttccc tggcccgaac
    30001 tcgttcaccg gcgaagatgt gctggaactg caaggtcatg gcggtccggt gatcctcgac
    30061 ctgctgttaa aacgcattct gaccattccc ggcctgcgga ttgctcgccc gggtgagttt
    30121 tccgaacgcg cgtttcttaa cgataaactc gacttagccc aggccgaggc gattgcggat
    30181 cttatcgacg ccagttcgga acaggcggcc cgttcggcac ttaactcgct gcaaggcgca
    30241 ttctccgcac gggttaatca tctggtagaa gccctcaccc acttgcgcat ttacgttgaa
    30301 gcggcaattg atttccccga tgaagagatc gatttcctct ccgacggcaa aattgaagcc
    30361 cagcttaatg acgtgattgc cgatctcgat gcagtgcgtg ctgaagcacg tcagggtagt
    30421 ttgttgcgcg aagggatgaa agtggtgatt gccggacgtc ctaacgccgg taaatcgagc
    30481 ctgttaaacg cgctggctgg ccgtgaagca gcaatcgtaa ccgatatcgc cggaactacg
    30541 cgtgacgtgt tgcgtgagca tatccacatt gacggaatgc cgctgcatat catcgacacc
    30601 gccgggctac gtgaagccag cgatgaagtg gaacgtattg gtatcgagcg cgcgtggcaa
    30661 gaaattgaac aagccgaccg cgtgctgttt atggtcgatg gcaccacgac agacgccgtt
    30721 gatccagcag agatctggcc ggagtttatc gcccgtctgc cagcgaaact gccgatcacc
    30781 gtggtgcgca ataaagccga tatcaccgga gaaacgttgg ggatgagcga agtgaacggt
    30841 cacgcgttaa ttcgtctctc tgcaaggact ggtgaaggtg tggacgtgct gcgtaaccat
    30901 ctcaaacaga gcatgggctt tgacaccaac atggaaggcg gcttcctggc gcgtcgtcgt
    30961 cacctacagg cactggaaca ggcggcagag cacctgcaac agggtaaagc gcaactgtta
    31021 ggagcctggg caggcgagct gctggcggaa gaactgcgtc tggcgcagca gaacttaagc
    31081 gagatcaccg gggaatttac ttctgacgac ctgctggggc gaattttctc cagcttctgt
    31141 attggtaagt aaccgcgctt gcgaagccgc attctgactg tcagatgcgg cttcgcttca
    31201 ttgttagcac tcctgttact cctcaac
//
LOCUS       DAZFLD010000029        31200 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.029, whole genome shotgun sequence.
ACCESSION   DAZFLD010000029 DAZFLD010000000
VERSION     DAZFLD010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31200
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.029"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            47..2470
                     /gene="pgaA"
                     /locus_tag="ACHKBL_001162"
     CDS             47..2470
                     /gene="pgaA"
                     /locus_tag="ACHKBL_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1901515 -
                     poly-beta-1,6-N-acetyl-D-glucosamine transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6 N-acetyl-D-glucosamine export
                     porin PgaA"
                     /protein_id="HFO5818061.1"
                     /translation="MYSSSRKRCPKTKWALKLLTAAFLAASPAAKSAVNNAYDALIIE
                     ARKGNTQPALSWFAQKSTLSNNQIADWLQIALWAGQDKQVITVYSRYRHQQLPARGYA
                     AVAVAYRNLQQWQNSLTLWQKALSLEPQNKDYQRGQILTLADAGHYDTALVKLKQLKS
                     GAPDKANLLAEAYIYKLTGRHQDELRAMTESLPENASKQQYPTEYVQALRNNQLAAAI
                     DDANLTPDIRADIHAELIRLSFMPTRSESERYAIADRALAQYAALEILWHDNSDRTAQ
                     YQRIQVDHLGALLTRDRYKDVISHYQRLKNTRQIIPPWAQYWVASAYLKEQQPKKAQS
                     IMTELFYHKETIAPDLSDEELADLFYSHLESENYPGALTVTQHTINTSPPFLRLMGTP
                     TSIPNDTWLQGHSFLSTVAKYSNDLPQAEMIARELAYNAPGNQGLRIDYASVLQARGW
                     PRAAENELKKAEVIEPRNINLEVEQAWTALTLQEWQQAAVLTHDVVEREPQDPGVVRL
                     KRAVDVHNLVELRIAGSTGIDAEGPDSGKHDVDLTTIVYSPPLKDNWRGFAGFGYADG
                     QFSEGKGIVRDWLAGVEWRSRNIWLEAEYAERVFNHEHKPGARLSGWYDFNDNWRIGS
                     QLERLSHRVPLRAMKNGVTGNSAQAYVRWYQNERRKYGVSWAFTDFSDSNQRQEVSLE
                     GQERIWSSPYLIVDFLPNLYYEQNTEHDTPYYNPIKTFDIVPAFEASHLLWRSYENSW
                     EQIFSAGVGASWQKHYGTDVVTQLGYGQRISWNDVIDAGATLRWEKRPYDGDREHNLY
                     VEFDMTFRF"
     gene            2479..4497
                     /gene="pgaB"
                     /locus_tag="ACHKBL_001163"
     CDS             2479..4497
                     /gene="pgaB"
                     /locus_tag="ACHKBL_001163"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine
                     N-deacetylase PgaB"
                     /protein_id="HFO5818062.1"
                     /translation="MLRNGNKYILMLVSIIMLTACISQSRTSFIPPQDRESLLAEQPW
                     PHNGFVAISWHNVEDEAADQRFMSVRTSALREQFAWLRENGYQPVSIAQIREAHRGGK
                     PLPEKAVVLTFDDGYQSFYTRVFPILQAFQWPAVWAPVGSWVDTPADEQVKFGDEMVS
                     REYFATWQQVREVARSRLVEVASHTWNSHYGIQANATGSLLPVYVNRAYFTDHARYET
                     AAEYRERIRLDAVKMTEYLRTKAEVNPHVFVWPYGEANGIAIAELKKLGYDMFFTLES
                     GLANASQLDSIPRVLIANNPSLKEFAQQIITVQEKPLQRVMHIDLDYIYDENRQQMDR
                     NIDVLIQRVKDMQISTVYLQAFADPDGDGLVKEVWFPNRLLPMKADIFSRVAWQLRTR
                     SGVNIYAWMPVLSWDLDPTLTRVKYLPTGEKKAQIHPEQYRRLSPFDDRVRAQVGMLY
                     DDLAGHAAFDGILFHDDALLSDYEDASAAAIAAYQQAGFSGSLSEIRQNPEQFKQWTR
                     FKSRALTDFTLELSARVKAIRGPHVKTARNIFALPVIQPESEAWFAQNYADFLKSYDW
                     TAIMAMPYMEGVTEKSADQWLTQLTNQMKSIPQAKDKSILELQAQNWQKNGQHQAISS
                     QQLAHWMSLLQLNGVKNYGYYPDNFLHNQPEIDVIRPEFSTAWYPKND"
     gene            4490..5815
                     /gene="pgaC"
                     /locus_tag="ACHKBL_001164"
     CDS             4490..5815
                     /gene="pgaC"
                     /locus_tag="ACHKBL_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008375 - acetylglucosaminyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042710 - biofilm formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine synthase"
                     /protein_id="HFO5818063.1"
                     /translation="MINRIVSFFILCLVLCIPLCVAYFHSGELMMRFVFFWPFFMSIM
                     WIIGGVYFWVYRERHWPWGENAPAPQLKDNPSISIIIPCFNEEKNVEETIHAALAQRY
                     ENIEVIAVNDGSTDKTRAILDRMAAQIPRLRVIHLAQNQGKAIALKTGAAAAKSEYLV
                     CIDGDALLDRDAAAYIVEPMLYNPRVGAVTGNPRIRTRSTLVGKIQVGEYSSIIGLIK
                     RTQRIYGNVFTVSGVIAAFRRSALAEVGYWSDDMITEDIDISWKLQLNQWTIFYEPRA
                     LCWILMPETLKGLWKQRLRWAQGGAEVFLKNMTRLWRKENFRMWPLFFEYCLTTIWAF
                     TCLVGFIIYAVQLAGVPLNIELTHIAATHTAGILLCTLCLLQFIVSLVIENRYEHNLA
                     SSLFWIIWFPVIFWMLSLATTLVSFTRVMLMPKKQRARWVSPDRGILRG"
     gene            5817..6230
                     /gene="pgaD"
                     /locus_tag="ACHKBL_001165"
     CDS             5817..6230
                     /gene="pgaD"
                     /locus_tag="ACHKBL_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine
                     biosynthesis protein PgaD"
                     /protein_id="HFO5818064.1"
                     /translation="MNNLIITTRQSPVRLLVDYVATTILWTLFALFIFLFAIDLLTGY
                     YWQSEARSRLQFYFLLAVANAVVLIVWALYNKLRFKKQQHHAAYQYTPQEYAESLAIP
                     DELYQQLQKSHRMSVHFTSQGQIKMVVSEKTLVRV"
     gene            complement(6280..7068)
                     /gene="phoH"
                     /locus_tag="ACHKBL_001166"
     CDS             complement(6280..7068)
                     /gene="phoH"
                     /locus_tag="ACHKBL_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PhoH"
                     /protein_id="HFO5818065.1"
                     /translation="MGRQKAVIKARREAKRVLRRDSRSHKQREEESVTSLVQMSGVEA
                     IGMARDSRDTSPILARNEAQLHYLKAIESKQLIFATGEAGCGKTWISAAKAAEALIHK
                     DVDRIIVTRPVLQADEDLGFLPGDIAEKFAPYFRPVYDVLVRRLGASFMQYCLRPEIG
                     KVEIAPFAYMRGRTFENAVVILDEAQNVTAAQMKMFLTRLGENVTVIVNGDITQCDLP
                     RGVRSGLSDALERFEEDEMVGIIRFGKEDCVRSALCQRTLHAYS"
     gene            complement(7694..8965)
                     /gene="efeB"
                     /locus_tag="ACHKBL_001167"
     CDS             complement(7694..8965)
                     /gene="efeB"
                     /locus_tag="ACHKBL_001167"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0033212 - iron import into cell [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter
                     deferrochelatase/peroxidase subunit"
                     /protein_id="HFO5818066.1"
                     /translation="MQYEDENGVNEPSRRRLLKGIGALALAGSCPVAHAQKTQSAPGT
                     LSPDARNEKQPFYGEHQAGILTPQQAAMMLVAFDVLASDKADLERLFRLLTQRFAFLT
                     QGGAAPETPNPRLPPLDSGILGGYIAPDNLTITLSVGHSLFDERFGLAPQMPKKLQKM
                     TRFPNDSLDAALCHGDVLLQICANTQDTVIHALRDIIKHTPDLLSVRWKREGVISDHA
                     ARSKGKETPINLLGFKDGTANPDSQNAKLMQKVVWVTADQQEPAWTIGGSYQAVRLIQ
                     FRVEFWDRTPLKEQQTIFGRDKQTGAPLGMQHEHDVPDYASDPEGKVIALDSHIRLAN
                     PRTPESESSLMLRRGYSYSLGVTNSGQLDMGLLFVCYQHDLEKGFLTVQKRLNGEALE
                     EYVKPIGGGYFFALPGVKDANDYLGSALLRV"
     gene            complement(8971..10098)
                     /gene="efeO"
                     /locus_tag="ACHKBL_001168"
     CDS             complement(8971..10098)
                     /gene="efeO"
                     /locus_tag="ACHKBL_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HFO5818067.1"
                     /translation="MTINFRRNALQLSVAALFSSAFMANAADVPQVKVTVTDKQCEPM
                     TITVNAGKTQFIIQNHSQKALEWEILKGVMVVEERENIAPGFSQKMTANLQPGEYDMT
                     CGLLTNPKGKLIVKGEATADAAQSDALLSLGGAITAYKAYVMAETTQLVTDTKAFTDA
                     IKAGDIEKAKALYAPTRQHYERIEPIAELFSDLDGSIDAREDDYEQKAADPKFTGFHR
                     LEKALFGDNTTKGMDKYADQLYTDVVDLQKRISELAFPPSKVVGGAAGLIEEVAASKI
                     SGEEDRYSHTDLWDFQANVEGSQKIVDLLRPQLQKVNPELLAKVDANFIKVDTILAKY
                     RTKDGFENYDKLTDADRNALKGPITALAEDLAQLRGVLGLD"
     gene            complement(10156..10986)
                     /gene="efeU"
                     /locus_tag="ACHKBL_001169"
     CDS             complement(10156..10986)
                     /gene="efeU"
                     /locus_tag="ACHKBL_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter permease EfeU"
                     /protein_id="HFO5818068.1"
                     /translation="MFVPFLIMLREGLEAALIVSLIASYLKRTQRGRWIGVMWIGVLL
                     AAALCLGLGIFINETTGEFPQKEQELFEGIVAVIAVVILTWMVFWMRKVSRNVKVQLE
                     QAVDSALQRGNHHGWALVMMVFFAVAREGLESVFFLLAAFQQDVGIWPPLGAMLGLAT
                     AVVLGFLLYWGGIRLNLGAFFKWTSLFILFVAAGLAAGAIRAFHEAGLWNHFQEIAFD
                     MSAVLSTHSVFGTLMEGIFGYQEAPSVSEVAVWFIYLIPALVAFALPPRAGATASRSA
                     "
     gene            complement(11652..13160)
                     /gene="putP"
                     /locus_tag="ACHKBL_001170"
     CDS             complement(11652..13160)
                     /gene="putP"
                     /locus_tag="ACHKBL_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005298 - proline:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0031402 - sodium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0015824 - proline transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HFO5818069.1"
                     /translation="MAISTPMLVTFCVYIFGMILIGFIAWRSTKNFDDYILGGRSLGP
                     FVTALSAGASDMSGWLLMGLPGAVFLSGISESWIAIGLTLGAWINWKLVAGRLRVHTE
                     YNNNALTLPDYFTGRFEDKSRILRIISALVILLFFTIYCASGIVAGARLFESTFGMSY
                     ETALWAGAAATILYTFIGGFLAVSWTDTVQASLMIFALILTPVIVIISVGGFGDSLEV
                     IKQKSIENVDMLKGLNFVAIISLMGWGLGYFGQPHILARFMAADSHHSIVHARRISMT
                     WMILCLAGAVAVGFFGIAYFNEHPAVAGAVNQNAERVFIELAQILFNPWIAGILLSAI
                     LAAVMSTLSCQLLVCSSAITEDLYKAFLRKQASQKELVWVGRVMVLVVALVAIALAAN
                     PENRVLGLVSYAWAGFGAAFGPVVLFSVMWSRMTRNGALAGMIIGALTVIVWKQFGWL
                     GLYEIIPGFIFGSIGIVVFSLLGKAPSAAMQKRFAEADAHYHSAPPSRLQEG"
     gene            complement(13319..13528)
                     /locus_tag="ACHKBL_001171"
     CDS             complement(13319..13528)
                     /locus_tag="ACHKBL_001171"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5818070.1"
                     /translation="MMLQLCATVLNVTCVASLKMNEMLINEIDMTGIKKITQTFSLRQ
                     LTFLKGATAKNVRECNLMKNSVAEH"
     gene            13583..17545
                     /gene="putA"
                     /locus_tag="ACHKBL_001172"
     CDS             13583..17545
                     /gene="putA"
                     /locus_tag="ACHKBL_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0004657 - proline dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trifunctional transcriptional regulator/proline
                     dehydrogenase/L-glutamate gamma-semialdehyde
                     dehydrogenase"
                     /protein_id="HFO5818071.1"
                     /translation="MGTTTMGVKLDDATRERIKSAATRIDRTPHWLIKQAIFSYLEQL
                     ENSDTLPELPALLSGAANESDEAPTPAEEPHQPFLDFAEQILPQSVSRAAITAAYRRP
                     ETEAVSMLLEQARLPQPVAEQAHKLAYQLADKLRNQKNASGRAGMVQGLLQEFSLSSQ
                     EGVALMCLAEALLRIPDKATRDALIRDKISNGNWQSHIGRSPSLFVNAATWGLLFTGK
                     LVSTHNEASLSRSLNRIIGKSGEPLIRKGVDMAMRLMGEQFVTGETIAEALANARKLE
                     EKGFRYSYDMLGEAALTAADAQAYMVSYQQAIHAIGKASNGRGIYEGPGISIKLSALH
                     PRYSRAQYDRVMEELYPRLKSLTLLARQYDIGINIDAEEADRLEISLDLLEKLCFEPE
                     LAGWNGIGFVIQAYQKRCPPVIDYLIDLATRSRRRLMIRLVKGAYWDSEIKRAQMDGL
                     EGYPVYTRKVYTDVSYLACAKKLLAVPNLIYPQFATHNAHTLAAIYQLAGQNYYPGQY
                     EFQCLHGMGEPLYEQVTGKVADGKLNRPCRIYAPVGTHETLLAYLVRRLLENGANTSF
                     VNRIADTSLPLDELVADPVTAVEKLAQQEGQTGLPHPKIPLPRDLYGDGRDNSAGLDL
                     ANEHRLASLSSALLNSALQKWQALPMLEQSVAAGEMSPVINPAEPKDIVGFVREATPR
                     EVEQALESAVNNAPIWFATPPAERAAILHRAAVLMESQMQQLIGILVREAGKTFSNAI
                     AEVREAVDFLHYYAGQVRDDFANETHRPLGPVVCISPWNFPLAIFTGQIAAALAAGNS
                     VLAKPAEQTPLIAAQGIAILLEAGVPPGVVQLLPGRGETVGAQLTGDDRVRGVMFTGS
                     TEVATLLQRNIASRLDAQGRPIPLIAETGGMNAMIVDSSALTEQVVIDVLASAFDSAG
                     QRCSALRVLCLQDEIADHTLKMLRGAMAECRMGNPGRLTTDIGPVIDSEAKANIERHI
                     QTMRSKGRQVFQAVRENSEDAREWQSGTFVAPTLIELDDFAELQKEVFGPVLHVVRYN
                     RNQLPALIEQINASGYGLTLGVHTRIDETIAQVTGSAHVGNLYVNRNMVGAVVGVQPF
                     GGEGLSGTGPKAGGPLYLYRLLANRPESALAVTLARQDAEYPVDAQLKAALTQPLNAL
                     REWAANRPELQALCTQYGELAQAGTQRLLPGPTGERNTWTLLPRERVLCIADDEQDAL
                     TQLAAVLAVGSQVLWPDDALHRQLVKALPSAVSERIQLAKAENITAQPFDAVIFHGDS
                     DQLRALCEAVAARDGAIVSVQGFARGESNILLERLYIERSLSVNTAAAGGNASLMTIG
                     "
     gene            complement(17585..18223)
                     /gene="rutR"
                     /locus_tag="ACHKBL_001173"
     CDS             complement(17585..18223)
                     /gene="rutR"
                     /locus_tag="ACHKBL_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator RutR"
                     /protein_id="HFO5818072.1"
                     /translation="MTQGAVKTTGKRSRAVSAKKKAILSAALDTFSQFGFHGTRLEQI
                     AELAGVSKTNLLYYFPSKEALYIAVLRQILDIWLAPLKAFREDFAPLAAIKEYIRLKL
                     EVSRDYPQASRLFCMEMLAGAPLLMDELTGDLKALIDEKSALIAGWVKSGKLAPIDPQ
                     HLIFMIWASTQHYADFAPQVEAVTGATLRDEVFFNQTVENVQRIIIEGIRPR"
     gene            18511..19602
                     /gene="rutA"
                     /locus_tag="ACHKBL_001174"
     CDS             18511..19602
                     /gene="rutA"
                     /locus_tag="ACHKBL_001174"
                     /EC_number="1.14.99.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein A"
                     /protein_id="HFO5818073.1"
                     /translation="MKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIVQKAEHYHFDF
                     ALSMIKLRGFGGKTEFWDHNLESFTLMAGLAAVTSRIQIYATAATLTLPPAIVARMAA
                     TIDSISGGRFGVNLVTGWQKPEYEQMGIWPGDDYFSRRYDSLTEYVQVLRDLWGSGKS
                     DFKGDFFTMDDCRVSPQPSVPMKVICAGQSDAGMAFSARYADFNFCFGKGVNTPTAFA
                     PTAARMKQAAEQTGRDVGSYVLFMVIADETDDAAHAKWEHYKAGADEEALSWLTEQSQ
                     KDTRSDTDTNVRQMADPTSAVNINMGTLVGSYASVARMLDEVASVPGAEGVLLTFDDF
                     LSGIENFGERIQPLMQCRAHLPALTQEVA"
     gene            19602..20294
                     /gene="rutB"
                     /locus_tag="ACHKBL_001175"
     CDS             19602..20294
                     /gene="rutB"
                     /locus_tag="ACHKBL_001175"
                     /EC_number="3.5.1.110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxyureidoacrylate/ureidoacrylate
                     amidohydrolase RutB"
                     /protein_id="HFO5818074.1"
                     /translation="MTTLTARPEAITFDPQQTALIVVDMQNAYATPGGYLDLAGFDVS
                     TTRPVIANIQTAVTAARAAGMLIIWFQNGWDEQYVEAGGPGSPNFHKSNALKTMRKQP
                     QLQGKLLAKGSWDYQLVDELMPQPGDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFTG
                     IATNVCVESTLRDGFFLEYFGVVLEDATHQAGPEFAQKAALFNIETFFGWVSDVETFC
                     DALSSTSFARIA"
     gene            20306..20692
                     /gene="rutC"
                     /locus_tag="ACHKBL_001176"
     CDS             20306..20692
                     /gene="rutC"
                     /locus_tag="ACHKBL_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-aminoacrylate deaminase"
                     /protein_id="HFO5818075.1"
                     /translation="MPKSVIIPAGSSAPLAPFVPGTLADGVVYVSGTLAFDQHNNVLF
                     ADDPKAQTRHVLETIRKVIETAGGTMADVTFNSIFITDWKNYAAINEIYAEFFPGDKP
                     ARFCIQCGLVKPDALVEIATIAHIAK"
     gene            20700..21500
                     /gene="rutD"
                     /locus_tag="ACHKBL_001177"
     CDS             20700..21500
                     /gene="rutD"
                     /locus_tag="ACHKBL_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein D"
                     /protein_id="HFO5818076.1"
                     /translation="MKLSLSPPPYADAPVVVLISGLGGSGSYWLPQLAVLVQEYQVVC
                     YDQRGTGNNPDTLAEDYSIAQMAAELHQALVAAGIERYAVVGHALGALVGMQLALDYP
                     ASVTVLVSVNGWLRINAHTRRCFQVREQLLHSGGAQAWVEAQPLFLYPADWMAARAPR
                     LEAEDALALAHFQGKNNLLRRLNALKRADFSHHADRIRCPVQIICASDDLLVPTACSS
                     ELHAALPDSQKMVMRYGGHACNVTDPETFNALLLNGLASLLHHREAAL"
     gene            21510..22100
                     /gene="rutE"
                     /locus_tag="ACHKBL_001178"
     CDS             21510..22100
                     /gene="rutE"
                     /locus_tag="ACHKBL_001178"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HFO5818077.1"
                     /translation="MNEAVSPGALSTLFTDARTHNGWRETPVSDETLREIYALMKWGP
                     TSANCSPARIVFIRTAEGKERLRPALSSGNLQKTLTAPVTAIVAWDSEFYERLPQLFP
                     HGDARSWFTSSPQLAEETAFRNSSMQAAYLIVACRALGLDTGPMSGFDRQYVDDAFFA
                     GSTLKSNLLINIGYGDSSKLFARLPRLSFEEACGLL"
     gene            22111..22605
                     /gene="rutF"
                     /locus_tag="ACHKBL_001179"
     CDS             22111..22605
                     /gene="rutF"
                     /locus_tag="ACHKBL_001179"
                     /EC_number="1.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309280.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent FMN reductase RutF"
                     /protein_id="HFO5818078.1"
                     /translation="MNIVDQQTFRDAMSCMGAAVNIITTDGPAGRAGFTASAVCSVTD
                     TPPTLLVCLNRGASVWPVFNENRTLCVNTLSAGQEPLSNLFGGKTPMEHRFAAARWQT
                     GVTGCPQLEEALVSFDCRISQVVSVGTHDILFCAIEAIHRHATPYGLVWFDRSYHALM
                     RPAC"
     gene            22626..23954
                     /gene="rutG"
                     /locus_tag="ACHKBL_001180"
     CDS             22626..23954
                     /gene="rutG"
                     /locus_tag="ACHKBL_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015218 - pyrimidine nucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006864 - pyrimidine nucleotide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization transport protein G"
                     /protein_id="HFO5818079.1"
                     /translation="MAMFGFPHWQLKSTSTESGVVAPDERLPFAQTAVMGVQHAVAMF
                     GATVLMPILMGLDPNLSILMSGIGTLLFFFITGGHVPSYLGSSAAFVGVVIAATGFNG
                     QGINPNISIALGGIIACGLVYTVIGLVVMKIGTRWIERLMPPVVTGAVVMAIGLNLAP
                     IAVKNVSASAFDSWMAVMTVLCIGLVAVFTRGMIQRLLILVGLIVACLLYGVMTNVLG
                     LGKAVDFTLVSHAAWFGLPHFSTPAFNGQAMMLIAPVAVILVAENLGHLKAVAGMTGR
                     NMDPYMGRAFVGDGLATMLSGSVGGSGVTTYAENIGVMAVTKVYSTLVFVAAAVIAML
                     LGFSPKFGALIHTIPAAVIGGASIVVFGLIAVAGARIWVQNSVDLSQNGNLIMVAVTL
                     VLGAGDFALTLGGFTLGGIGTATFGAILLNALLSRRLVDVPPPEVVHQEP"
     gene            complement(24037..24210)
                     /gene="ymdF"
                     /locus_tag="ACHKBL_001181"
     CDS             complement(24037..24210)
                     /gene="ymdF"
                     /locus_tag="ACHKBL_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HFO5818080.1"
                     /translation="MANHRGGSGNFAEDRERASEAGKKGGQHSGGNFKNDPQRASEAG
                     KKGGKSSHGKSDN"
     gene            25171..25440
                     /locus_tag="ACHKBL_001182"
                     /pseudo
     CDS             25171..25440
                     /locus_tag="ACHKBL_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000203836.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="antA/AntB antirepressor family protein"
     gene            25707..26330
                     /locus_tag="ACHKBL_001183"
     CDS             25707..26330
                     /locus_tag="ACHKBL_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antA/AntB antirepressor family protein"
                     /protein_id="HFO5818081.1"
                     /translation="MTTQLIPVFEGTISNEPTLLVNARDLHGFLEVGKDFSNWIRARI
                     NEYGFVEHLDYILFSPNLAKTLGRRRKDYHLTLDTAKELAMVERNEKGRQIRRYFIEC
                     EKKLRNIQPVQTELQFTAEEIILLCYMQLWMEKAQDLSKHLYPIMKELNSSYTNKLYD
                     IAFETIYMVTKNKEALLREAAQLDQSSFVVQRARPMLKSLRARQFEF"
     gene            26378..26599
                     /locus_tag="ACHKBL_001184"
     CDS             26378..26599
                     /locus_tag="ACHKBL_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HFO5818082.1"
                     /translation="MADIIDNAAEIEELQRNLSLQKYKSDSNAPSATHCCECGDPIDE
                     RRRLAVRGCRACASCQQDIELINKQRGVK"
     gene            26596..26880
                     /locus_tag="ACHKBL_001185"
     CDS             26596..26880
                     /locus_tag="ACHKBL_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4752 family protein"
                     /protein_id="HFO5818083.1"
                     /translation="MSIDTTITIDTALNTGLALLGYFYIMFCAGRWLSLLLLKKWNKC
                     RKQDQRQKAMNAFSEAFGIDSMEPGDPARVISRGDVVILVYRSQEKNERD"
     gene            26867..27196
                     /locus_tag="ACHKBL_001186"
                     /pseudo
     CDS             26867..27196
                     /locus_tag="ACHKBL_001186"
                     /inference="COORDINATES: protein motif:HMM:NF025303.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
     gene            27518..27703
                     /locus_tag="ACHKBL_001187"
                     /pseudo
     CDS             27518..27703
                     /locus_tag="ACHKBL_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309543.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
     gene            27708..27896
                     /locus_tag="ACHKBL_001188"
     CDS             27708..27896
                     /locus_tag="ACHKBL_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5818084.1"
                     /translation="MNRKIIVTTVISVPANAPHQDIDDFVESKFGQDKDMKPDNLCYG
                     DAAEIISHHWEYGSHYMN"
     gene            27984..28202
                     /locus_tag="ACHKBL_001189"
     CDS             27984..28202
                     /locus_tag="ACHKBL_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4014 family protein"
                     /protein_id="HFO5818085.1"
                     /translation="MVTLFRKKYPRKSRATEFLFLILFIVLMIPISPLLLVWFIGKII
                     EPVIELYNDVVWASFNTLHNKINPYKEN"
     gene            28204..28491
                     /locus_tag="ACHKBL_001190"
     CDS             28204..28491
                     /locus_tag="ACHKBL_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5818086.1"
                     /translation="MATLQELIDLTPEQEKAWNRLVKAVKDFRAAGGKFYSVLDTLSA
                     YNGEHVASIDNDKGYHTASVYMPSIDAPGLTSWADDWHGITLKDGVEVDED"
     gene            28495..28617
                     /locus_tag="ACHKBL_001191"
                     /pseudo
     CDS             28495..28617
                     /locus_tag="ACHKBL_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416864.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            28855..29124
                     /locus_tag="ACHKBL_001192"
     CDS             28855..29124
                     /locus_tag="ACHKBL_001192"
                     /inference="COORDINATES: protein motif:HMM:NF016343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HFO5818087.1"
                     /translation="MRHGPEPVSQTCKLNELSSNSPVTPARWISCSTRIPAQDDWILI
                     YSKHGEYMAGQVQGEYVELSDGTLSWLRSALFWMPLPEPPKEVRQ"
     gene            29310..30494
                     /locus_tag="ACHKBL_001193"
     CDS             29310..30494
                     /locus_tag="ACHKBL_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001637813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFO5818088.1"
                     /translation="MIFTRIKLVNLYAFCNAELNLSYPKKQVNIPLEGEHLAGRPKFY
                     YKKVCIITGGNASGKTSLGRILCGIQIFLRTKELRSSLRLNHKDLDASFEVDFATENF
                     LHHRLYVRFAANETGTLSVKEVKYGYVKININDTCFKTTEKLNKLFSVGVPTPAEYYF
                     HEISSDSNAVTLEPFKNVIFSGGWYYQLSGTQESTTEISRMNKDILERVIRTFDSSIV
                     GVVEMTESESEDKNPSRVYLIRFKNKDYSIVTSKGDITNSERMSRGTYDAVTIAHFVS
                     AIISDCEELGVRSDIPSMTYYLDEKLAYSHSELERIIVTLIISKLKENTQFFYTTHNM
                     DIFELDLPVHSFIFLRKDKDCSEFIEAGSILKKNDRSIRSAVARDIFDVMPDMSLVSE
                     LL"
     gene            30496..31029
                     /locus_tag="ACHKBL_001194"
     CDS             30496..31029
                     /locus_tag="ACHKBL_001194"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5818089.1"
                     /translation="MARRATVAIVFVEGETELSLFKKMKRDNIIAVKEVVKKNFWQDR
                     IHKYAIAIPKGSDIFVVFDSDQVEQSARFIENVRFLRSRGHRVYLFQQTSNFEEELSW
                     CCNKSIPRLIYDFCGKKTSGVNDFKRDFIACNNSPDKLIKLGIREHLWFSRALHDVLK
                     PVADMKSNFSRHFRLAK"
BASE COUNT         7947 a         7782 c         7826 g         7645 t
ORIGIN      
        1 aattagatat agagagagat tttggcaata catggagtaa tacaggatgt attcaagtag
       61 cagaaaaagg tgcccgaaaa ccaaatgggc tttgaaactt cttactgccg catttttagc
      121 agcgagtccc gcggcgaaga gtgctgttaa taacgcctat gatgcattga ttattgaagc
      181 tcgcaaagga aatactcagc cagctttgtc atggtttgca caaaaatcaa cactcagtaa
      241 taaccaaatt gctgactggt tacaaattgc cttatgggcc gggcaagata aacaggtcat
      301 taccgtttac agccgctacc gtcatcagca attaccagcg cgtggttatg cagctgtcgc
      361 cgtcgcttat cgtaacctgc aacaatggca aaactcgctt acactgtggc aaaaggcgct
      421 ctctctggag ccgcaaaata aggattatca acggggacaa attttaaccc tggcagatgc
      481 gggtcactat gatactgcgc tggttaaact taagcagctt aaatctggag cgccggacaa
      541 agccaattta ctcgcagaag cctatatcta taaactgacg gggcgtcatc aggatgaatt
      601 acgggcgatg acagagtcat tacctgaaaa tgcatctaag caacaatatc ccacagaata
      661 cgtgcaggca ttacgtaata atcaactagc tgccgcgatt gacgatgcca atttaacgcc
      721 agatattcgc gctgatattc atgccgaact gatcagactg tcgtttatgc ctacgcgcag
      781 tgaaagtgaa cgttatgcca ttgccgatcg cgccctcgcc caatacgctg cattagaaat
      841 tctgtggcac gataactcag accgcactgc ccagtaccag cgtattcagg ttgatcatct
      901 tggcgcgtta ttaactcgcg atcgttataa agacgttatt tctcactatc agcgtttaaa
      961 aaatacgagg caaattattc cgccctgggc gcaatattgg gttgcgtcgg cttatctcaa
     1021 agaacagcag ccaaaaaaag cacagtcaat aatgacagag ctcttttatc acaaggagac
     1081 cattgccccg gatttatccg atgaagaact tgcggatctc ttttacagcc acctggagag
     1141 tgaaaattat cccggcgcgc taactgtcac ccaacatacc attaatactt cgccgccttt
     1201 ccttcggtta atgggcacgc ctacgagcat cccgaatgat acctggttac aggggcattc
     1261 gtttctctca accgtagcaa aatatagtaa tgatcttcct caggctgaaa tgatagccag
     1321 agagcttgct tataacgcac caggaaatca gggactgcgc attgattacg cgagtgtgtt
     1381 acaagcccgc ggttggcctc gtgcagcaga aaatgaatta aaaaaagcag aagtgatcga
     1441 gccacgtaat attaatctgg aggttgaaca agcatggaca gcattaacgt tacaagaatg
     1501 gcagcaggca gctgtcttaa cgcacgatgt tgtcgaacgt gaaccgcaag atcccggcgt
     1561 tgtacgttta aaacgtgcgg ttgatgtaca taatcttgta gagcttcgta tagctggttc
     1621 aacaggaatt gatgccgaag gcccggatag tggtaaacat gatgtcgact taaccaccat
     1681 cgtttattca ccaccgctga aggataactg gcgcggtttt gctggattcg gttatgccga
     1741 tggacaattt agcgaaggaa aagggattgt tcgcgactgg ctggcgggtg ttgagtggcg
     1801 gtcacgtaat atctggctcg aggcggagta cgctgaacgc gttttcaatc atgagcataa
     1861 acccggcgcg cgcctgtctg gctggtatga ttttaatgat aactggcgta ttggttcgca
     1921 actggaacgc ctctctcacc gcgttccatt acgggcaatg aaaaatggtg ttacaggcaa
     1981 cagtgctcag gcttatgttc gctggtatca aaatgagcgg cgtaagtacg gtgtctcctg
     2041 ggctttcact gatttttccg acagtaacca gcgtcaggaa gtctcacttg agggtcagga
     2101 acgcatctgg tcttcaccat atttgattgt cgatttccta cccaatctgt attacgaaca
     2161 aaatacagaa cacgacactc catactacaa ccctataaaa acgttcgata ttgttccggc
     2221 atttgaggca agtcatttgt tatggcgaag ctatgaaaat agctgggagc aaatcttcag
     2281 cgcaggtgtt ggtgcctcct ggcaaaaaca ttatggcacg gatgtcgtca cccaactcgg
     2341 ctacgggcaa cgtattagct ggaatgacgt gattgatgct ggcgcaacgc tacgctggga
     2401 aaaacgacct tatgacggtg acagagaaca caacttatac gttgaattcg atatgacatt
     2461 cagattttaa ggataaatat gttacgtaat ggaaataaat atatcctgat gctggtgagt
     2521 ataattatgc tcactgcgtg cattagccag tcaagaacat catttatacc gccacaggat
     2581 cgcgaatctt tactcgccga gcaaccgtgg ccgcataatg gttttgtggc gatttcatgg
     2641 cataacgttg aagacgaagc tgccgaccag cgttttatgt cagtgcggac atcagcactg
     2701 cgtgaacaat ttgcctggct gcgcgagaac ggttatcaac cggtcagtat tgctcaaatt
     2761 cgtgaagcac atcgaggagg aaaaccgcta ccggaaaaag cagtagtgct gacttttgat
     2821 gacggctacc agagttttta tacccgcgtc ttcccaattc ttcaggcctt ccagtggcct
     2881 gctgtatggg cccccgtcgg cagttgggtc gatacgccag cggatgaaca agtaaaattt
     2941 ggcgatgaga tggtcagtcg agaatatttt gccacgtggc aacaagtgcg agaagttgcg
     3001 cgttcccggc tcgttgaggt cgcttctcat acatggaatt ctcactacgg tattcaggct
     3061 aatgccaccg gcagcttatt gcctgtatat gtaaatcgtg catattttac tgaccacgca
     3121 cggtatgaaa ccgcagcaga ataccgggaa agaattcgtc tggatgctgt aaaaatgacg
     3181 gaatacctgc gtacaaaggc tgaggtaaat ccacacgttt ttgtttggcc ttatggcgaa
     3241 gcgaatggca tagcaatcgc ggaattaaaa aaactcggtt atgacatgtt cttcaccctt
     3301 gaatcaggtt tggcaaatgc gtcgcaatta gattccattc cgcgagtatt aatcgccaat
     3361 aatccctcat taaaagagtt tgcccagcaa attattaccg tacaggaaaa accactacaa
     3421 cgggtaatgc atatcgatct tgattacatt tatgacgaaa accgccagca aatggatcgc
     3481 aatattgatg tgctaattca gagggtaaaa gatatgcaaa tatctaccgt gtatttacaa
     3541 gcatttgctg atcccgatgg tgatgggctg gttaaagagg tctggtttcc aaatcgtttg
     3601 ctaccaatga aagcagatat ttttagtcgg gttgcctggc aattgcgtac ccgctcaggt
     3661 gtaaacatct atgcgtggat gccggtatta agctgggatt tagatcccac attaacgcga
     3721 gtaaaatact taccaactgg ggagaaaaaa gcacaaattc atcctgaaca ataccgccgt
     3781 ctctcccctt tcgatgacag ggtcagagca caagttggca tgttatatga tgatcttgcc
     3841 ggacatgctg cttttgatgg catattgttc cacgatgatg ctttgctttc agattatgaa
     3901 gatgccagtg cagcggctat cgcggcttat cagcaagctg gctttagcgg gagtctgagc
     3961 gaaattcgac aaaacccgga gcaattcaaa cagtggaccc gctttaaaag tcgtgcgtta
     4021 actgacttca ctttagaact tagtgcgcgc gtaaaagcca ttcgtggtcc acatgttaaa
     4081 actgcacgaa atatttttgc acttccggta atacaacctg aaagtgaagc ttggtttgca
     4141 cagaattatg ctgatttcct aaaaagctat gactggaccg ctattatggc tatgccttat
     4201 atggaaggcg tcacagaaaa atcggctgat caatggttaa cacaattgac caatcagatg
     4261 aaaagcatcc cgcaggctaa agacaaatca attttagaat tacaggcaca aaactggcag
     4321 aaaaatggtc agcatcaggc tatttcttca caacaactcg ctcactggat gagcctatta
     4381 caactgaatg gagtgaaaaa ttatggttat tatcccgaca attttctgca taaccaacct
     4441 gaaatagacg ttattcgtcc tgagttttca acagcctggt atccgaaaaa tgattaatcg
     4501 catcgtatcg ttttttatat tatgtctggt gttatgcata cctctatgcg tagcgtactt
     4561 tcactctggt gaactgatga tgaggttcgt tttcttctgg ccgtttttta tgtccattat
     4621 gtggattatt ggcggcgtct atttctgggt ctatcgtgaa cgccactggc cgtggggaga
     4681 aaacgcacca gccccccagt tgaaagataa tccatctatc tccattatca ttccctgttt
     4741 taatgaggag aaaaacgttg aggaaaccat acatgccgct ttagcacagc gttatgagaa
     4801 cattgaagtt attgccgtaa atgatggttc aacagacaaa acccgtgcca tcctggatcg
     4861 catggctgca caaattcccc gtctgcgggt cattcatctg gcgcaaaacc aggggaaagc
     4921 cattgcgctt aaaaccggag ctgccgcggc gaaaagtgaa tatctggtgt gcattgatgg
     4981 cgacgcgtta ttagaccgcg atgcagcggc atatattgtg gaaccgatgt tgtacaaccc
     5041 acgtgtgggt gccgtaaccg gtaatcctcg tattcgcaca cgttctaccc tagtgggtaa
     5101 aattcaggtt ggcgagtatt cctcaattat tggtttgatc aagcggaccc agcgtatcta
     5161 tggaaatgta tttaccgttt ccggtgttat tgccgcattt cgtcgcagcg ccctggcaga
     5221 agtgggttac tggagtgacg atatgatcac cgaagatatt gatattagct ggaagctgca
     5281 gttgaatcag tggacgattt tttacgagcc acgggcgctg tgctggatat taatgcctga
     5341 aacgttaaaa gggctgtgga aacagcgcct gcgctgggct cagggcggtg cagaagtatt
     5401 cctcaaaaat atgacaaggt tgtggcgaaa agaaaacttt cgaatgtggc cactgttttt
     5461 tgaatactgc ctgacgacaa tatgggcctt tacctgcctg gtcggtttca ttatttacgc
     5521 agtccaactt gcaggtgtac cgttaaatat tgaattgaca catatcgcag cgacacatac
     5581 tgccggaata ttattgtgca cgttatgttt actgcaattt attgtcagtc tggtaattga
     5641 gaatcgctat gagcacaatc tggcttcatc gcttttctgg attatttggt ttccggttat
     5701 tttctggatg ctaagcctgg caacgacatt ggtatcattt acacgagtca tgttgatgcc
     5761 taaaaaacaa cgcgctcgtt gggtaagtcc cgatcgtggg attctgagag gttaatatga
     5821 acaatctaat tattacgacc cgacaatcgc cagtacgttt actggttgat tatgttgcca
     5881 caaccatctt gtggacatta tttgctttgt tcatattctt attcgccatt gatctgctga
     5941 cgggttatta ctggcaaagc gaggccagaa gccgacttca gttctatttt ttgctggcag
     6001 tggcgaatgc cgtcgtgtta atcgtctggg cattgtacaa taagctgcgt tttaaaaaac
     6061 agcagcatca tgcagcatac caatatacgc cgcaagaata tgcagagagc ttagcaatac
     6121 ctgatgagct ttatcagcaa ctacaaaaaa gccacaggat gagcgtacac ttcaccagtc
     6181 agggacaaat aaaaatggtg gtttcagaaa aaacgctagt ccgggtataa acacccaaaa
     6241 caaagcccgg ttcgcccggg ctctgcaccg ataatacact taactgtatg catgtagcgt
     6301 acgttggcaa agtgccgaac gtacgcaatc ctctttaccg aatcggatga tcccgaccat
     6361 ttcatcttct tcgaaacgtt ccagcgcgtc acttaatccg gagcgcacac cgcgaggtaa
     6421 atcgcattgc gtgatatcac cgttgacgat aaccgtcacg ttctccccga ggcgggttaa
     6481 aaacattttc atttgcgcgg cagtcacatt ctgcgcctcg tcaagaatga cgactgcatt
     6541 ttcaaaggta cgtccacgca tataggcgaa cggcgcgatt tcgaccttcc ctatttctgg
     6601 tcgcaggcag tactgcataa aggaagcccc taagcgccgg accagcacgt cgtaaaccgg
     6661 gcgaaaatag ggagcaaact tttctgcgat atctccaggt aaaaagccaa gatcttcatc
     6721 ggcttgcaga actggacggg tgacgataat cctgtcgaca tccttatgta tcagggcctc
     6781 tgccgctttt gctgcgctga tccaggtttt tccgcacccg gcttcgcccg tggcgaatat
     6841 cagctgctta ctctcaatag ccttcagata gtgcaattgc gcttcatttc gcgcgaggat
     6901 gggcgaggta tcgcgactgt cgcgggccat accaatggct tctacgccgc tcatttgcac
     6961 aagcgaggtg accgattctt cttcacgctg cttatggctg cgcgaatccc gtctcagcac
     7021 acgttttgcc tcgcgacgag ctttgatcac tgctttttgt cttcccatgg agagcacctt
     7081 gagttgtttg tattcatcac acgcgccgtt ggcagcgcga ttatgcgcac gaacatcaga
     7141 gggttggctt ccttgtaagc catagtttgc ttttggataa aatgccgaaa aacggctacg
     7201 cacaccgttt gcggcgtcgg taacacgagt aaagagagga agtgagaaaa atacagagtg
     7261 acgaagttgc taccggagga gaaatctccg cgagtggtgg agccttgctt aacaaaacat
     7321 tgtccagaac aggacgttac catccgcgat ctccatagtg actgactatc actgccggga
     7381 acttccgctg ctacttaata agtacaacag atctcgcatt cattgcaaca atatattcac
     7441 ttatatttaa ccataaaaca ccatttcagt gacattagtt tctactggaa agatgacaaa
     7501 gtgatgacag tgataaaaaa gctgtgtgct ttcagcagga ttcgcagtac tggtaaaagc
     7561 ttgccggatg cggcgtgaac gccttatccg gcctaccgaa cccgcaccga ttgtaggcat
     7621 gataagacgc gcaagcgtcg catcagcgca cgaacgcctg gtgcggcgta aacgccttat
     7681 ccgcctaaaa acattaaacc cgcaataatg cgcttccgag ataatcgttc gcgtccttca
     7741 cacccggcag cgcaaagaaa tagccgccac cgataggttt gacatattcc tccagcgcct
     7801 ctccattcaa ccttttttgt actgtcagga agcccttttc cagatcgtgt tggtagcaga
     7861 caaacagtag ccccatatcg agttgcccgg aattggtgac gcccaacgaa taactgtagc
     7921 cacgacgcag catcagactt gactcactct ctggcgtgcg gggattcgcc agccggatat
     7981 ggctgtccag cgcgatcacc ttcccctccg gatcgctggc gtaatcaggc acatcatgct
     8041 catgctgcat tcccagcggc gcgccggttt gcttatcacg cccaaatatc gtctgctgtt
     8101 ctttcagcgg cgttctgtcc caaaattcca cccgaaactg aatcaaccgc accgcctggt
     8161 agctaccacc gattgtccac gcaggctcct gctgatctgc cgtcacccac accacttttt
     8221 gcatcaactt cgcattctgg ctatcgggat tggcagtgcc gtctttgaaa cccagcaaat
     8281 taatcggtgt ctctttacct ttactacgcg ccgcgtggtc ggaaataacc ccttcccgct
     8341 tccagcgcac gctgagcaaa tccggcgtgt gtttgatgat atcgcgcagc gcatggatca
     8401 ccgtgtcctg ggtgttggcg caaatttgta gcaacacatc accatgacat aacgccgcat
     8461 ccagcgagtc gttggggaaa cgcgtcatct tctgcaactt tttcggcatc tgtggcgcaa
     8521 ggccaaagcg ctcatcaaac aatgagtggc ccaccgataa cgttatggtg agattatcgg
     8581 gcgcaatgta gccgccaaga atgccggaat cgagtggtgg caggcgcgga tttggcgttt
     8641 ctggtgccgc tccgccctga gtcagaaaag caaaacgctg agtcaacaag cgaaacaacc
     8701 gttcaagatc ggctttatcg ctggcaagca catcaaacgc caccagcatc attgcagcct
     8761 gttgtggcgt caggatccct gcctgatgct caccataaaa cggctgtttc tcattgcgtg
     8821 catccggtga aagcgtaccc ggcgcacttt gcgttttttg tgcatgagcg accggacaac
     8881 ttcccgccag cgccagcgca ccaatccctt tcagtaaacg tcggcgtgac ggttcattca
     8941 cgccgttttc atcttcatac tgcataacgc ttagtccagc cccagcacac cgcgaagttg
     9001 cgccagatct tccgccagcg cagtaatcgg tcctttcagt gcattccggt cagcatcggt
     9061 cagtttgtcg taattttcaa aaccgtcttt agtacggtat ttcgccagaa tggtatcgac
     9121 ctttatgaag ttggcatcga cttttgccaa cagttccgga ttcacttttt gtagttgtgg
     9181 acgcagcaga tcgacaattt tctgcgagcc ttcaacgtta gcctggaaat cccacagatc
     9241 ggtgtggctg tagcgatctt cttcaccgct gattttgctg gccgccactt cctcaatcag
     9301 tccggctgca ccgccgacca cttttgaagg agggaaagcc agttcactga tgcgtttttg
     9361 caaatcgacc acatcggtat aaagctggtc ggcatactta tccatccctt tggtggtgtt
     9421 atcgccaaac aatgcttttt ccagacggtg gaaaccggtg aatttcggat cggcggcttt
     9481 ttgctcgtaa tcatcttcac gggcgtcaat gctgccatcc agatcggaga acagttcagc
     9541 aatcggttca atgcgctcat agtgctggcg cgtcggcgca tacagtgctt tcgctttttc
     9601 gatatcgcct gctttaatag catcggtaaa ggctttggtg tcggtcacca gctgcgtggt
     9661 ttccgccatg acatacgctt tatatgcagt aattgcacca ccaagactta acagcgcatc
     9721 actttgcgcc gcatccgccg ttgcctcacc tttgacgatc aacttccctt tcgggtttgt
     9781 cagcagaccg caggtcatat cgtattcacc aggctgtaaa tttgccgtca ttttctggct
     9841 aaaaccaggg gcgatatttt cccgctcttc caccaccatc acgcctttga ggatctccca
     9901 ctccagtgct ttctggctgt ggttctgaat aatgaactgt gttttcccgg cgttaaccgt
     9961 aatggtcatc ggttcgcact gcttatccgt cacggtcact ttgacctgcg gcacatcagc
    10021 ggcgttagcc ataaaagcag aagaaaacag cgcagccacg ctcaactgca atgcgttacg
    10081 gcggaagtta atggtcatga cgagactatc cctttaaagt atgttgtaac taagcaagag
    10141 tttgcgtcgt atttgttacg cggagcgaga cgctgtcgcc cctgcgcgtg gtggcagagc
    10201 aaatgccacc agcgccggga tgagataaat aaaccagacg gcgacttcgc tgacgctcgg
    10261 cgcttcctga tagccaaaaa tcccttccat cagcgtgcca aacaccgagt gagttgagag
    10321 caccgcactc atatcgaagg cgatttcctg aaagtggttc cacaatccgg cttcatgaaa
    10381 tgcgcgaatg gcaccagccg ccagccctgc ggcgacgaag agaataaaca ggctggtcca
    10441 tttaaaaaat gcaccaagat tgaggcgaat accgccccag tagagcagga agccgagcac
    10501 cacggctgta gcaagaccga gcattgcacc cagcggcggc cagatcccga catcttgttg
    10561 aaacgccgcc agcaggaaaa agaccgactc cagcccttcc cttgcaacgg caaaaaagac
    10621 catcatcacc agcgcccagc catgatgatt tccacgctgc aatgcgctat cgactgcctg
    10681 ctccagttgt actttgacgt tgcgcgacac tttgcgcatc cagaaaacca tccaggtaag
    10741 gatcaccacg gcgatcaccg ccacgatacc ttcaaacagt tcctgttctt tttgcggaaa
    10801 ttcgccggtg gtttcgttaa tgaagatacc caagcccagg cacaacgcag cggcaagcaa
    10861 cacgccaatc cacatcacac caatccatcg gcctcgctgg gtacgcttaa gatagctggc
    10921 aatcaaactg acaatcagcg cggcttcaag tccttcgcgc aacataatga gaaacggaac
    10981 aaacatgcca cccaccctta aacgtttttc tgagtcaatc tatgcaaaga aaagtaaatc
    11041 gcagtgatag cgattatcat tacggtaaag aaaaatacaa gcagaatgtt tataaaatga
    11101 tgttggaatg taaacaaaga aaggtaatgc caggggatat aaacgattat tccaggtaca
    11161 aagatgggat tgtttacaac gagccatgtg ttcttgccag atgccgcgta aatgccttat
    11221 ccagccgaca aaactgtcca gactcaatgc atcagagctt ccatgtaaaa ctgataagcg
    11281 ggtgtatcaa gcaatttcgt ccatccgcga ttatcaaatc taactttttg cccggaacat
    11341 aataacagct cgggtgttgg tggcgtcaaa atcgctgaag cgttccccga aggccggggc
    11401 agcccacatg gatgtgggct gagggcgcgt cttacaggac gttacctcgc gcccgacccg
    11461 gtagccgaag gggataagct gaaggtaccg cgcagcggcg attttgttcg ccagagcccg
    11521 ggggtgcagg gggcggcggc gattggccgc cccctgcgcg ctccctgcac cattcaattt
    11581 atatggcgag gcacattaag ggagcgcaac ccgctgctga agccaaacaa aaccgccgca
    11641 ggctaagtcc cttacccttc ctgcaaccgt gacggcggag ccgaatgata gtgcgcatcg
    11701 gcctcggcaa agcgtttttg catcgccgct gacggcgctt tacccagcaa actaaacact
    11761 acaatcccga tactgccgaa gataaagccc ggaataattt cgtacagacc cagccagccg
    11821 aactgtttcc agacgataac cgtcagcgca ccgatgatca tccccgccag cgcaccgttg
    11881 cgcgtcatgc gtgaccacat caccgagaac agcaccaccg gaccaaacgc tgcgccaaag
    11941 cctgcccacg cgtagctcac taagcccagc acgcggtttt ccgggtttgc cgccagcgca
    12001 atcgccacca gcgccaccac cagcaccatc actcgcccta cccacaccag ctctttctgg
    12061 ctggcctgtt tacgcagaaa cgctttgtat aaatcttcgg taatcgcact ggaacatacc
    12121 agcagctggc aacttaacgt tgacattacc gccgccagaa tcgccgacag caaaatcccg
    12181 gcaatccacg ggttaaacag aatttgcgcc agttcaataa acacgcgttc agcgttctgg
    12241 tttaccgcac ctgctaccgc cgggtgctcg ttaaagtaag cgataccaaa gaagccgaca
    12301 gccaccgccc ctgccaggca gaggatcatc caggtcatac tgatacgacg cgcatggaca
    12361 atgctgtggt gagaatctgc cgccataaaa cgcgccagga tgtgcggctg cccgaagtaa
    12421 cccagccccc accccatcag cgagataatg gcgacaaaat tcaacccttt gagcatatcc
    12481 acgttttcga tgctcttttg tttgattact tccagcgagt cgccaaaccc accgacgcta
    12541 atgatcacga taaccggcgt caggatcagg gcaaaaatca tcaggctggc ctgtacagtg
    12601 tcagtccagc tcaccgcgag gaaaccgcca ataaaggtgt aaaggatcgt cgccgcagcc
    12661 cccgcccaca gagccgtttc gtagctcatg ccaaaggtac tttcaaacag acgcgcgcct
    12721 gccacaatgc ccgaagcgca ataaatggtg aagaacagca aaataaccag cgcggagata
    12781 atgcgcaaaa tgcggctttt atcttcaaag cgcccggtga aataatccgg caatgttaag
    12841 gcgttattgt tgtattcggt atgcacacgc aaccgcccgg ccaccagctt ccagtttatc
    12901 cacgcgccta atgtcaggcc aatggcgatc cagctttcgg aaatcccgga aagaaaaacc
    12961 gcgcccggca accccattaa cagccagccg ctcatatccg acgctcccgc cgataatgcc
    13021 gtcacgaatg gcccaagact acgaccgccc agaatatagt cgtcgaagtt tttcgttgat
    13081 cgccaggcga taaacccaat caatatcatg ccaaagatat agacacaaaa tgtcaccaac
    13141 atcggtgtgc taatagccat ctaaagtctc caaaaaatta ttatcggcaa tgtcgaaact
    13201 tgccgttata tctgccaccg gaacggggta acagagttta tgttttacca gggcgaccgt
    13261 atcctgccgg aagcgctggt tattcacaat cgatttaaca caccatttac attaaatttt
    13321 agtgctcagc gacactattt ttcatcaggt tgcactctct cacatttttt gcggttgcac
    13381 ctttcaaaaa tgttaactgc cgcagagaaa aagtctgagt tattttttta atccctgtca
    13441 tatcgatttc gtttattaac atttcattca tttttaagct tgctacgcag gtcacattta
    13501 acacagttgc acaaagttgc aacatcatgg atatttcacg ataacgttaa gttgcacctt
    13561 tcagaacaac aggagtaatg gcatgggaac caccaccatg ggggttaagc tggacgacgc
    13621 gacgcgtgag cgtattaagt ctgccgcgac acgtatcgat cgcacgccac actggttaat
    13681 taaacaggcg attttttctt atcttgaaca actggaaaac agcgatactc tgccggagct
    13741 acctgcgctg ctttctggcg ctgccaatga gagcgatgaa gcaccgactc cggcagagga
    13801 accacaccag cctttcctcg actttgccga gcaaattttg ccccagtcgg tttcccgcgc
    13861 cgcgatcacc gctgcctatc gccgtccgga aaccgaagcg gtttcgatgc tgctggaaca
    13921 agctcgcctg ccgcagccag ttgctgaaca ggcgcacaaa ctggcgtatc aactggccga
    13981 taaactgcgt aatcaaaaaa acgccagtgg tcgcgcaggt atggttcagg ggttattgca
    14041 ggagttttcg ttgtcatcgc aggaaggcgt ggcgctgatg tgtctggcag aagcgttgtt
    14101 gcgtattccc gacaaagcca cccgtgatgc gttaattcgc gacaaaatca gcaacggtaa
    14161 ctggcagtca cacattggtc gtagcccgtc gttgtttgtt aatgccgcca cctgggggct
    14221 gctgtttacc ggcaaactgg tttccaccca taacgaagcc agcctctccc gctcgctgaa
    14281 ccgcattatc ggtaagagcg gtgaaccgct gatccgcaaa ggtgtggata tggcgatgcg
    14341 cctgatgggc gagcagttcg tcacgggtga aaccattgca gaagcgttag ccaatgcacg
    14401 caagctggaa gagaaaggtt tccgttactc ctacgatatg ctgggcgaag ccgcgctgac
    14461 cgccgcagat gcacaggcgt atatggtttc ctatcagcag gcgattcacg ccattggtaa
    14521 agcgtccaat ggtcgtggca tctatgaagg tccgggcatt tcaatcaagc tgtcggcgct
    14581 gcatccgcgt tacagccgcg cccagtatga ccgggtaatg gaagagcttt acccacgtct
    14641 gaaatcactc accctgctgg cgcgtcagta cgatattggt attaacatcg atgccgaaga
    14701 ggccgatcgt ctggagatct ccctcgatct gctggaaaaa ctctgtttcg agccggaact
    14761 ggcaggctgg aacggcatcg gttttgttat tcaggcttat caaaaacgct gcccaccggt
    14821 gattgattac ctgattgatc tcgccacccg cagccgtcgc cgtctgatga ttcgcctggt
    14881 gaaaggcgcg tactgggata gtgaaatcaa gcgcgcgcag atggacggcc ttgaaggtta
    14941 tccggtttat acccgcaagg tgtataccga cgtttcatat ctcgcctgtg cgaaaaagct
    15001 gctggcggtg ccgaatctaa tctacccgca gttcgcaacg cataacgccc atacgctggc
    15061 ggcaatttat caactggcgg gtcagaacta ctacccgggt cagtacgagt tccagtgcct
    15121 gcacggtatg ggcgagccac tgtatgagca ggtcaccggg aaagttgccg acggcaaact
    15181 taaccgtccg tgtcgtattt atgctcctgt cggtacccat gaaacgctgc tggcgtatct
    15241 ggtgcgtcgc ctgctggaaa acggtgctaa cacctcgttt gttaaccgta ttgctgacac
    15301 ctctttgcca ctggatgaac tggttgccga tccggtcact gctgtagaaa aactggcgca
    15361 acaggaaggg caaactggat taccgcatcc gaaaattccc ctgccgcgcg atctttacgg
    15421 tgacgggcgc gacaactcgg cagggctgga tctcgccaat gaacaccggc tggcctcgct
    15481 ctcttctgcc ctgctcaata gtgcactgca aaaatggcag gccttgccaa tgctggaaca
    15541 atcggtcgcg gctggtgaga tgtcgcccgt tattaaccct gcggaaccga aagatattgt
    15601 gggctttgtg cgtgaagcca cgccgcgtga agtagaacag gcgctggaaa gtgcggttaa
    15661 taacgcgcca atctggtttg ccactcctcc ggctgaacgc gcggcgattt tgcatcgcgc
    15721 tgccgtgcta atggaaagcc agatgcagca actgattggt attctggtgc gtgaagccgg
    15781 aaaaaccttc agtaacgcca ttgccgaagt gcgcgaagcg gtcgattttc tccactacta
    15841 tgccggacag gtgcgggatg atttcgctaa cgaaacccac cgtccattag ggcctgtggt
    15901 atgtatcagt ccgtggaact tcccgctggc tattttcacc gggcagatcg ctgccgcact
    15961 ggcggcaggt aacagcgtgc tggcaaaacc ggcagaacaa acgccgctga ttgccgcgca
    16021 agggatcgcc attttgctgg aagcgggtgt accgccaggc gtggtgcaat tgctgccagg
    16081 tcggggtgaa accgtgggcg cgcaactgac gggtgatgat cgcgtgcgcg gggtgatgtt
    16141 taccggttca accgaagtcg ctacgttact gcagcgcaat atcgccagcc gcctggacgc
    16201 tcagggtcgc cctattccgc tcatcgctga aaccggcggc atgaacgcga tgattgtcga
    16261 ttcttcagca ctgaccgaac aagtcgtaat agatgtgctg gcctcggcgt tcgacagtgc
    16321 aggtcagcgt tgttcggcac tgcgcgtgct gtgcctgcaa gatgagattg ccgaccacac
    16381 gctgaaaatg ctgcgcggcg caatggccga atgccggatg ggtaatccgg gtcgcctgac
    16441 caccgatatc ggtccggtga ttgatagcga agcgaaagcc aatattgagc gtcatattca
    16501 gaccatgcgt agtaaaggcc gtcaggtgtt ccaggcggtg cgggaaaaca gcgaagatgc
    16561 ccgtgaatgg caaagcggca cctttgtcgc tccgacgctg atcgaactgg atgactttgc
    16621 cgaattacaa aaagaggtct ttggtccggt gttgcatgtg gtgcgttaca accgtaacca
    16681 gctgccggcg ctgatcgagc agattaacgc ttccggttat ggtctgacgc ttggcgtcca
    16741 tacgcgcatt gatgaaacca tcgcccaggt cactggctcg gcgcatgtcg gtaacctgta
    16801 cgttaaccgt aatatggtgg gcgcagtggt tggggtgcaa ccgttcggcg gcgaagggtt
    16861 gtccggtacc gggccgaaag caggcggtcc gctctatctc taccgtctgc tggcgaatcg
    16921 cccggaaagt gcgctggcag tgacgctcgc gcgtcaggat gcggagtatc cggtcgatgc
    16981 gcagttgaaa gccgcattga ctcagccgct aaatgcactg cgggaatggg cggcaaaccg
    17041 tccagaattg caggcgttat gtacgcaata tggcgagttg gcgcaggcag gaacacaacg
    17101 attgttgcca gggccgacgg gtgaacgcaa cacctggacg ctgctgccgc gtgagcgcgt
    17161 gttgtgtatt gcggatgatg aacaggatgc gctgactcag ctcgccgccg tgctggcggt
    17221 gggtagccag gtgctgtggc cggatgatgc gctgcatcgt cagttagtga aggcattgcc
    17281 atcggcagtc agcgaacgta ttcaactggc gaaagcggaa aatataaccg ctcaaccatt
    17341 tgatgcggtg atcttccacg gtgattcgga tcagcttcgc gcattgtgtg aagccgttgc
    17401 cgcgcgggat ggtgcaattg tttcggtgca gggttttgcc cgtggcgaaa gcaatatcct
    17461 tctggaacgg ctgtatatcg agcgttcgct gagtgtgaat accgctgctg ctggcggtaa
    17521 cgccagttta atgacgatcg gttaaacagg tagccggagg atgttacaac ctcctccggc
    17581 atctttaacg tggtcgaatc ccctcaataa taatccgctg cacgttttca accgtttgat
    17641 tgaaaaacac ctcatcgcgc aacgtcgcgc ctgtcaccgc ctccacctga ggggcaaaat
    17701 cggcgtaatg ttgagtggaa gcccaaatca taaaaatcaa atgctgcggg tcaatcggcg
    17761 cgagtttgcc gcttttaacc caaccggcaa tgagcgccga tttctcatca attaatgcct
    17821 tcaaatcgcc cgtcagttca tccattaaca gcggtgcacc tgccagcatc tccatacaga
    17881 acaggcgcga agcctgcgga taatcgcggg aaacttccag cttcagacgg atgtattctt
    17941 tgatcgccgc cagcggggcg aaatcttctc gaaacgcttt taacggtgcc agccagatat
    18001 cgagaatctg ccgcagtacg gctatataca gcgcctcttt tgacggaaag taatacagca
    18061 gattggtttt tgaaacaccc gccaactccg cgatctgctc cagccttgtg ccgtgaaaac
    18121 cgaattgtga aaaagtgtcc agtgctgcgc taagaatcgc ttttttcttc gcgcttactg
    18181 cgcgcgaacg cttacccgtt gttttcactg cgccttgcgt catgcgctct cccctcttgt
    18241 tgattggcaa caggatagca aaacccccgc ccggcctcga ccttctgcac tctcatcgcg
    18301 cacgaatgca tgttttatgt gcaactgttt tgaccgttta gtccactttt tttcaggcat
    18361 tttagacaac ttaacattaa cacattaata acattcactt tttaaaaact ggcatccgct
    18421 ttgcaaacaa gccattacgc ggctgaaaag gatatgcagg atgcagcgcc ccgtctgact
    18481 ttcactttac gagacgaaga gaggttgatg atgaaaattg gcgtattcgt acctattggc
    18541 aacaacggct ggctcatttc gacccacgcg ccgcagtaca tgccgacatt tgaactgaat
    18601 aaagccattg tgcaaaaagc ggagcactac catttcgatt tcgccctgtc gatgatcaaa
    18661 ctgcgtggct ttggcggcaa aactgagttc tgggatcaca accttgagtc gttcaccttg
    18721 atggcggggc tggcagccgt cacctcacgc attcagatat acgcgacagc tgccacctta
    18781 acgttacctc cggcaatcgt cgcccgtatg gccgcaacca tcgactccat ctctggcggg
    18841 cgttttggcg tcaacctcgt gactggctgg cagaagcccg agtatgagca gatggggatc
    18901 tggcctggcg atgactattt ctcccgtcgt tacgactctc tcaccgaata tgttcaggtg
    18961 ctgcgcgacc tgtggggctc ggggaaaagc gattttaaag gcgatttttt cactatggat
    19021 gattgtcgcg tcagtccgca accgagtgtc cccatgaaag tgatctgcgc cgggcaaagc
    19081 gacgctggca tggcgttctc cgcccggtat gccgatttta acttctgttt cggcaaaggc
    19141 gtaaatacac ccacggcttt cgccccgacc gctgcgcgga tgaaacaggc cgcagagcaa
    19201 accggacgcg acgttggctc ttatgtgttg tttatggtga ttgccgacga aaccgacgat
    19261 gccgctcatg caaaatggga acactacaaa gcgggcgcgg atgaagaggc gttaagctgg
    19321 ctaaccgaac aaagtcagaa agatacccgc tccgatactg acaccaacgt ccgtcagatg
    19381 gccgatccca cttcggcggt aaacatcaat atggggacgt tagtcggttc ttacgccagt
    19441 gtcgcgcgca tgttagatga agtcgcaagc gtgcctggtg ccgaaggcgt gctgttgacc
    19501 ttcgatgatt ttctgtcggg aatcgaaaac ttcggcgagc gcattcaacc actgatgcag
    19561 tgccgcgccc atctccctgc gctgactcag gaggtggcat gatgacgacc ttaaccgcac
    19621 gaccggaagc cattaccttc gatccgcagc aaactgcgct gatcgtggtg gatatgcaaa
    19681 acgcttatgc cacgccaggc ggctacttag atctcgccgg gtttgatgtc tcaaccactc
    19741 gcccggtcat tgccaacatt caaaccgccg tgaccgcagc gcgagcggca gggatgttga
    19801 tcatctggtt tcaaaatggc tgggatgaac agtatgtcga ggctggcggc cccggctcac
    19861 cgaattttca taaatcgaac gccctgaaaa ccatgcgtaa gcagccgcag ctgcagggga
    19921 aattgctggc gaaaggctcc tgggattatc aactggtgga tgaactgatg ccgcagcctg
    19981 gcgatattgt gctgccgaag ccgcgctaca gcggtttctt caatacgccg ctggacagca
    20041 ttttgcgcag ccgtggaata cgccatctgg ttttcaccgg tatcgctacc aacgtctgcg
    20101 tcgaatcgac gctacgcgac ggcttttttc tggagtattt cggcgtggtg ctggaagacg
    20161 caacacacca ggcgggaccg gaatttgcac agaaagccgc gttgttcaat atcgaaacct
    20221 tttttggctg ggtcagcgac gtcgaaacgt tctgcgacgc gctttcttcc acgtcctttg
    20281 ctcgtatcgc ttaaggagtt taacgatgcc aaaatccgta attattcctg ctggcagcag
    20341 cgcaccgctg gcccccttcg ttcccggcac gctggctgat ggcgtggtgt atgtctccgg
    20401 tacgctggct tttgatcaac ataacaacgt gctgtttgcc gatgacccaa aggcgcaaac
    20461 ccgccacgtt ctggaaacca tccgcaaggt catcgagacg gcgggtggca caatggcgga
    20521 tgtgaccttc aacagcatct ttattaccga ctggaaaaat tacgccgcga ttaacgaaat
    20581 ctacgccgaa tttttcccgg gtgacaaacc ggcgcgattc tgcattcagt gcggactggt
    20641 aaaacctgac gcgctggtgg aaatcgccac aattgcgcat atcgccaagt gaggccgcga
    20701 tgaaactttc actctcacct cccccttatg ctgatgcgcc cgtagtggtg ttgatttcgg
    20761 gtcttggcgg tagcggcagt tactggttac cgcaactggc ggtgttggtt caggagtatc
    20821 aggtagtctg ttacgaccag cgcggcaccg gcaataatcc cgacacgctg gcagaagatt
    20881 acagtatcgc ccagatggca gcggaactgc atcaggcgct ggtagccgca gggattgagc
    20941 gttacgcggt ggtcggccat gcgctcggtg cgctggtggg aatgcagctg gcgctggatt
    21001 atcccgcgtc ggtaactgtg ctggtcagcg ttaacggctg gctacgaata aacgcccata
    21061 cgcgccgctg ttttcaggtt cgcgaacagt tactacatag cggcggcgcg caggcatggg
    21121 tggaagcgca gccgttgttc ctctatcccg ccgactggat ggcggcccgc gcacctcgcc
    21181 ttgaggcaga agacgcgctg gcactggcgc attttcaagg caaaaataat ttactgcgtc
    21241 gacttaacgc cctcaaacgc gctgacttta gtcaccatgc ggatcgtatc cgctgcccgg
    21301 tgcaaatcat ctgcgccagt gatgatctgc tggtgccaac agcatgttcc agtgaacttc
    21361 atgccgccct gcccgatagc cagaaaatgg tgatgcgcta tggcggacac gcctgcaacg
    21421 tgaccgatcc cgaaacgttt aatgctctgt tactcaacgg gcttgccagc ctgttacatc
    21481 accgtgaagc cgctctgtaa ggaattgcta tgaacgaagc cgttagccca ggtgcgctta
    21541 gcaccctgtt caccgatgcc cgcactcaca acggctggcg ggagacaccc gtcagcgatg
    21601 agacgttacg ggagatttat gccctgatga aatgggggcc gacatcagct aactgttctc
    21661 cggcacggat cgtgtttatc cgcactgcag aaggaaaaga acgtctgcgc ccggcgcttt
    21721 ccagcggcaa tctgcaaaaa accctgaccg cgcccgtcac cgctatcgtc gcctgggaca
    21781 gtgaatttta tgaacggtta ccacagctgt ttcctcacgg cgatgcccgc agttggttta
    21841 cctccagtcc acaacttgcc gaagaaacag cgtttcgcaa cagttccatg caggcggcct
    21901 atctgatcgt cgcctgccgt gcgctggggc tggataccgg cccgatgtcg ggctttgacc
    21961 gtcaatacgt tgacgacgcc ttttttgcgg gcagcacgct gaagagcaat ctgctgatta
    22021 atatcggcta tggcgattcc agcaaacttt ttgcgcgcct gccacgtctg tcctttgaag
    22081 aagcctgcgg gctgttgtaa ggagcaaaca atgaacattg tcgatcaaca aacctttcgc
    22141 gatgcgatgt cctgtatggg cgcggcggtc aatatcatca ctacggacgg tccagccggg
    22201 cgcgccgggt tcaccgccag cgccgtctgc agcgtgactg acacgccgcc cacattactg
    22261 gtgtgcctga atcgtggggc gtccgtctgg ccggtattca atgaaaatcg aacgctgtgt
    22321 gtaaatacac ttagcgccgg gcaggagccg ctttcaaacc tttttggcgg caaaacgccc
    22381 atggaacacc gctttgccgc cgcccgctgg cagactggtg tgaccggatg cccgcaactg
    22441 gaagaggcac tggtttcgtt tgactgccgt atcagccagg tggtgagcgt cggcacccac
    22501 gacattttgt tttgcgccat cgaagcgatt catcgtcacg ccacacccta cgggctggtg
    22561 tggttcgatc gcagttatca cgcgctgatg cgccctgctt gttaacctcc ttaaggagac
    22621 agctgatggc aatgttcggt tttcctcact ggcagttaaa atcgacctct acagagagcg
    22681 gcgtggtcgc gccggatgaa cgattgccgt ttgctcaaac ggcagtaatg ggcgttcaac
    22741 acgcggtggc gatgtttggc gcgacggtgt taatgccaat tctgatgggg ctggatccca
    22801 atctttccat tttaatgtcg gggatcggca cgttactgtt tttctttatt accggcgggc
    22861 acgtgcccag ttatctcggt tccagcgcgg cttttgtcgg cgtggtgatt gccgcgacgg
    22921 gctttaatgg tcagggaatt aatccgaata tcagcatcgc gctgggcggg attatcgcct
    22981 gtgggctggt ttataccgtc attggcctgg tggtgatgaa aatcggtacg cgctggattg
    23041 aacgactaat gccaccggtt gtgacaggtg cggtagtgat ggcgattggc ctgaacttag
    23101 cgcctatcgc agtgaaaaat gtatcggctt cggcctttga tagctggatg gcggtaatga
    23161 cggtgctgtg tattgggctg gtggccgtat ttacccgtgg catgatccag cgattattga
    23221 ttctcgtagg attaattgtc gcctgtctgc tgtatggcgt gatgaccaac gttttagggc
    23281 tgggaaaagc cgtcgatttt actctcgtca gccatgctgc ctggttcggt ctgccacatt
    23341 tttccactcc tgcttttaat ggtcaggcaa tgatgctgat tgcgcccgtg gcggtgattc
    23401 tggtggcgga aaacttaggt catctcaagg cggtcgccgg gatgaccgga cgcaatatgg
    23461 acccgtatat ggggcgggca ttcgtaggcg atgggctggc gacgatgctt tctggctctg
    23521 tcggcggtag cggcgtaacc acctacgccg agaatattgg tgtgatggcc gtgacaaaag
    23581 tctactcgac gctggtgttt gttgccgctg ccgtcatcgc catgctgttg ggcttttcgc
    23641 cgaaatttgg cgcgctgatc catactattc cggccgcggt gattggtggt gcatcaattg
    23701 ttgtgttcgg gctgattgcc gtcgcaggcg cacggatctg ggtacaaaac agtgtggatt
    23761 taagccagaa cggtaatttg attatggtcg cagtaacctt agtgctgggt gcgggtgatt
    23821 ttgctctcac actgggcggt tttacgttgg gagggatcgg tacagccacc tttggcgcga
    23881 ttttactcaa tgcgttgtta agcagaaggt tggttgacgt tccaccgcca gaagttgttc
    23941 atcaggagcc ataaatagaa aaccgccggg gtaaccggcg gtgatgactt tttgacgagt
    24001 ttcagacaaa aatacattcg tcatggatta gcccggctag ttgtcgcttt taccgtgact
    24061 gctcttgcct ccttttttac ctgcttcaga tgcgcgctgc ggatcatttt tgaaattacc
    24121 cccgctgtgc tgtccacctt ttttacctgc ttctgatgct ctttcgcggt cttctgcaaa
    24181 attgccggaa ccgcctcgat ggtttgccat tactgacctc cgtctgggta tagacatcat
    24241 attgcataga attaacgcgg cgtatggcgg ctgccatatt ttcgctacgt gggactaagc
    24301 ttagtgaact gacgcgggca gacccgcgtt tagtaatatc ctgcaacagc ttcttagcga
    24361 gaatagatat catctccaat aattatccat aagccgctct tatgattaaa aaaacgcgat
    24421 gagataaaaa tgtgtcattt tgcgacaaaa ttacgtgctt gtgaaagttg ttataaatca
    24481 aataagtggt tgtgaaattt gcactctgaa aaggacgtct tatctttaaa taagtggtag
    24541 cgaatcgcta cggaatagag ataacacgag gagtggttag aaatggctaa agttctggtg
    24601 ctttattatt ccatgtacgg acatattgaa accatgaagc attaaaaaca cagaattatc
    24661 gaagatcgtc caagagcata tatttttcaa tctgttacgc ctcattttga ccatgagaaa
    24721 atcgatttca caccgttctt tatacgttct tttttgcccc aaatatgccc caaacaacca
    24781 cactttgccc caatagtgcc ccaaagattt aaggggattt aacttgatat ttcagataat
    24841 taacgcaagt ttgcgatgtg aatttacgtt aatttactgg atgaggcatt tactgccacc
    24901 cgttcatcaa tatttccgca gattgacaaa tccgccagag ctatcgcata ctgatcgcac
    24961 tgaacaaata agcggtctcc gcacccgata gctttgcggc ttttttatgc ctgcaatttg
    25021 gcatagttac atccgtacaa aggtcgggtg gagaggcgta atacaacacc cgcaagggga
    25081 atatgcccag agcttcttat ttggctcagt tgacacccga ccgccagcta ctaactggcg
    25141 gttataaact aaacaaataa ggaggtcatc atgaccagtc aactcatccc cgtattcaac
    25201 ggttctatcg acaacgaaac aactcttcta gttaacgctc gtgatctaca ctcttttctt
    25261 ggtgtgggca gaatgttcgc gcactgggtt aaagagcgca ttgctgaata tggattcgtt
    25321 gaaagcctgg actatatttt gatttgccaa aatgggcaaa ccaaaggtcg aggaggtgat
    25381 cgccgtagca aggactacca cctcacactg gacacagcca aagagctggc gatggttgac
    25441 gactgcgtcc aatactgact atactccgcg ccgaggcctc gaaaacctcc caaaagcgga
    25501 ttaaaccaac cccgtcagtg ttggattttt tatgcctgtt attcagtgat agcacagtgt
    25561 gcggtcacat ccccgatcaa tgtcgggagg gcgacgaata caacacccgc aaggggaata
    25621 agtccgcggt atcttttggg ccgtttcgag cctcccggca ccacatttgt ggtggcattt
    25681 ctcgaaaaaa gccaaaggag gtcataatga ccactcaact catccctgtg tttgaaggta
    25741 caatctcaaa tgaacctacg cttctggtga acgcgcgtga tttgcatgga tttttagagg
    25801 taggtaaaga tttttctaac tggatacggg ccaggataaa cgagtatgga ttcgtagaac
    25861 atttagacta tattctattt tcgccaaatt tggcgaaaac tctaggccgc cgccgcaagg
    25921 actaccacct aactctcgac acagccaaag aactggcgat ggttgagcgt aacgaaaaag
    25981 gccgccagat ccgccgctac ttcatcgagt gcgaaaagaa acttcgcaac attcagccag
    26041 tacaaactga gctgcaattc acagccgagg aaatcatcct cctctgctac atgcaactct
    26101 ggatggaaaa agcccaggac ctcagcaaac acctgtatcc gatcatgaaa gagctgaact
    26161 catcatacac gaacaagctg tatgacattg cgtttgagac tatctacatg gtgacgaaga
    26221 acaaggaagc actactgcgg gaggcggcac agcttgacca gtcaagtttc gttgtccagc
    26281 gggccagacc aatgctgaaa agcctgcggg caaggcaatt cgaattctaa aaccaaagga
    26341 gcttcggctc cttttttcat gcctgaagga aaggaaaatg gcagatatca tcgacaacgc
    26401 agctgaaatc gaagaattgc agcgcaatct ctccctgcaa aaatacaaat ccgatagtaa
    26461 tgccccatct gctactcatt gttgcgagtg tggcgatccg atagatgagc ggcgacgcct
    26521 ggctgttcgt ggatgccgag cttgcgcaag ttgccagcag gatattgaac ttatcaacaa
    26581 acagagaggt gtgaagtgag catagacacc actataacga tcgatacggc cctcaacaca
    26641 ggtctggcgc ttcttggtta tttttacatt atgttctgcg ccgggcggtg gctatcgctg
    26701 ttgttactga aaaaatggaa taaatgccgt aagcaggatc aacgccagaa ggcaatgaat
    26761 gcgttttccg aagccttcgg gattgacagc atggagccag gagatccagc tcgcgtaatt
    26821 agcagaggtg acgttgtaat tcttgtatac cggagccaag agaaaaatga gcgagattaa
    26881 ctatcaggta ctgcgtgaaa aggcagaaaa agcaacgcgt ggtgagtggt cgctcgaata
    26941 tggagagaac cgatttgatg gtgatgatgc actaattcat cgcgaggctg ctggatatat
    27001 tcccatttgc agaattgaag gagcacatcc tgaaagcggt ttcgatgaag atttccaaat
    27061 ggaacagcag gccaatgctg aattcatcgc cgcagccaat ccggctaccg tgctggcact
    27121 gctggatgaa cgggaaagaa accagcaata catcaaacgc cgcgaccagg agaacgagga
    27181 aattgctctt acggttggga agctgcgtgt tgagcttgag gaagtaaaac aacacgctga
    27241 agaattatcc gaaaccaagg ctgttcgtaa ccaatggcgg ccagatattt gcccaataac
    27301 cggacgtaca tttttcatgt ggattgagca tccaacattg ggaaatgtgc cgacatatgg
    27361 tggcccatta gatagttaca ccattccaac aaaggacggt gacggtgagt tttcatgtga
    27421 gcgttacgat catgattttg gcggttgggt agaaagcgaa tgtcttgggt tatatctgat
    27481 tgatgataga gaacaatgca gggtctacga actggaggaa cgcgttaaag agctggaaac
    27541 gcgggaagtt catttgccga ctcgctacgg ccttcgatat ggacatccga taaatgatga
    27601 tgagcgccac gtcatgatac ctaaagaaaa tggctgctgg ctttatctgg ctgacttaga
    27661 acatgcacta cgtgttgctg gcattcgcat caaaggaggg tgagaaagtg aatcgaaaaa
    27721 taatagtgac aactgtaatt tcagtccctg ccaatgcccc gcatcaggat attgatgatt
    27781 ttgttgaaag taaattcggg caagacaaag atatgaaacc ggataattta tgttatggag
    27841 atgctgccga aataattagc catcattggg agtatggttc gcactacatg aattaattac
    27901 atcatattat taatttaatc cttaaccgga gggatttctg caccctcaga acatcgggag
    27961 gccgcccgaa agggcggtaa tgaatggtca cattatttag aaaaaaatat ccgcgaaaaa
    28021 gtagagcgac agaatttttg tttctcattc tgtttatcgt gttgatgata ccgatatccc
    28081 cgctattact ggtctggttt atcgggaaaa taattgaacc agttattgag ttgtataacg
    28141 acgtggtatg ggcgtcgttc aacacactgc acaataaaat taacccatat aaggaaaact
    28201 gaaatggcaa ctttgcagga attaatcgac ctgacgccag aacaggaaaa agcgtggaat
    28261 cgtcttgtga aggctgtaaa ggatttcagg gcagccggag gaaagtttta tagtgtccta
    28321 gacacgctga gcgcatacaa cggcgagcac gttgccagca ttgataacga taagggctac
    28381 cacactgcaa gcgtctatat gcctagcatt gatgcgccag ggttaaccag ttgggctgat
    28441 gattggcacg gcatcacgct gaaagatggc gttgaagtgg atgaggacta acacatgaca
    28501 acgttcaccg acaaagaaat gattaaagaa atcaaagagc gcataggcag cctggacgtt
    28561 cgagacaata ttgaacgccg tgcttatgaa attgcgctgg catcgctgga agcagaggct
    28621 gtgtgcgtta tagaccagtc aaatcttgat tatcttgagg ctggctatga tgcagacgtg
    28681 tggccggtat ccagagcaga aatggctgat gtgtttctgt atcgtgcagt tccgccagtg
    28741 ccggataaaa tcgcgttagc aattgaaaat ctcaagcaga aattagtgga atgcaatcgc
    28801 tataactact gcgcagatgc agttaaaggc gttgaggatg cttacctcgc tgccatgcgt
    28861 catggtccag aacctgtaag ccaaacttgc aagttgaacg agctgtcgag caactctccg
    28921 gtaactccgg ctcgttggat aagctgtagt acacgaatcc ccgctcaaga tgattggatt
    28981 ttaatttatt caaagcacgg cgagtatatg gcaggacagg tacaagggga atacgtggag
    29041 ttgagcgacg gcactttatc gtggttaagg agcgccttat tctggatgcc gctgccagaa
    29101 ccgccgaagg aggtacgcca atgagctagc ctgaagcctt ctcaaatgtt ggtattgcaa
    29161 tggcggtagc gctgattgtg tacacaattt gccacagggg gaggcatttc cgcgccaagc
    29221 aatatcggat gtaggtccct ataaattttg ttgacgttac tgacttcctg tacaatactc
    29281 aactttagtt ccaaaacatg gaagaaaaaa tgattttcac gcgcataaaa cttgtaaatc
    29341 tgtatgcatt ctgcaacgca gaactgaatc tttcataccc caaaaagcag gtgaatatac
    29401 cgcttgaggg tgaacatctg gctggcagac ctaaatttta ttataagaaa gtctgtatca
    29461 ttactggtgg gaacgcttcc ggaaaaacct cgctgggcag aatattgtgt gggattcaga
    29521 tttttctcag aaccaaagag ctgcgctcca gtctgcgact taaccacaag gatctggatg
    29581 cctcctttga agtggatttt gcaacagaga acttcctgca tcacaggttg tatgttcgct
    29641 ttgcagcaaa tgaaacaggc acactttctg ttaaagaagt gaaatatggc tatgtgaaga
    29701 taaacataaa tgatacctgt tttaaaacaa cagaaaagct aaacaaacta ttttctgtgg
    29761 gagttccaac tcctgccgaa tattatttcc atgaaatttc ttctgatagt aatgctgtta
    29821 ctctcgaacc cttcaagaac gttatttttt caggaggatg gtattaccaa ctatcaggaa
    29881 cccaagaaag cacgactgaa atttcaagaa tgaataaaga tattcttgag agagtaattc
    29941 gcacttttga ttcaagtatt gttggcgtcg ttgaaatgac cgaatctgag tcagaagaca
    30001 aaaacccctc cagagtttat ttgataagat ttaaaaacaa ggattattcg attgtaacaa
    30061 gtaaaggaga tataacgaac tcagagcgta tgtcacgggg tacatacgac gctgtcacaa
    30121 tagctcattt tgtcagtgcg ataatctctg attgtgaaga acttggcgta cgctcagata
    30181 ttccgtcaat gacctattat cttgatgaga agttagccta ctcgcattct gaactggagc
    30241 ggataatcgt tacgctgatt atttccaagc ttaaggaaaa cactcagttt ttctatacca
    30301 cacataacat ggatattttt gagcttgacc tgcctgttca ttcgttcatt tttctcagaa
    30361 aggataagga ttgttctgag ttcattgaag ctggctcaat cctgaaaaaa aacgacagga
    30421 gtatacgaag cgctgttgca cgagatatct tcgacgttat gcctgatatg tctttagttt
    30481 cggagttgct ttaaaatggc aagaagagca acagttgcca tcgttttcgt cgaaggcgaa
    30541 actgaattat ctttgttcaa aaaaatgaag agagataata ttatcgcagt gaaggaggtt
    30601 gttaaaaaga acttctggca ggatcggata cataagtatg cgatagcgat accaaagggt
    30661 agtgatatat tcgttgtgtt tgattctgat caggttgagc aatcggcgag atttatagaa
    30721 aatgtaagat ttttacgttc cagaggacac cgggtttatt tgtttcagca aacaagtaat
    30781 tttgaagaag agcttagctg gtgctgcaat aaatccattc ctaggttgat ttatgatttt
    30841 tgcggaaaga aaacatccgg agttaatgat ttcaaaaggg attttatagc atgcaataat
    30901 tcaccagaca aattgattaa gctgggtatc cgggaacacc tgtggttttc ccgcgctcta
    30961 cacgatgtgt taaaacctgt tgcagatatg aagtcaaatt tttcccggca tttccgtctg
    31021 gcaaaataag gacgggaatc agtgaaatcg gcccgatgta aactgtgtcg ggcatcctca
    31081 acacacccgc ccaatccaat tagccccgca cttccgtacg gggcatgttc tttactccac
    31141 tcccactttt ttgttgtact cgcggttaaa ccgatccacg gcaattttca tatcccgctc
//
LOCUS       DAZFLD010000030        30312 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.030, whole genome shotgun sequence.
ACCESSION   DAZFLD010000030 DAZFLD010000000
VERSION     DAZFLD010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30312
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.030"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(373..663)
                     /gene="nadS"
                     /locus_tag="ACHKBL_001195"
     CDS             complement(373..663)
                     /gene="nadS"
                     /locus_tag="ACHKBL_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000356400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NadS family protein"
                     /protein_id="HFO5818090.1"
                     /translation="MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRAS
                     GLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALLRAIANDPQHVLQALNR"
     gene            complement(664..975)
                     /locus_tag="ACHKBL_001196"
     CDS             complement(664..975)
                     /locus_tag="ACHKBL_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000897300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO5818091.1"
                     /translation="MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGG
                     LRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIYRKGIKDDLSPQEKAMLRLLNTR
                     W"
     gene            1204..2112
                     /locus_tag="ACHKBL_001197"
     CDS             1204..2112
                     /locus_tag="ACHKBL_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFO5818092.1"
                     /translation="MAQEKGIAELVEEFIAAGRPSSRQQSIAQRREGYIASAVLAGET
                     ETRVDIQTIELEAMTLRIVSPLNAPTLLPTIIYYYGGCFVSGGFATHDNQLRQLAYYG
                     QCRVIAVQYRLAPEHTFPAAHDDAQRGAELVWQHAERLGVDKQRITLAGDSAGGHLAL
                     VTALRLKRAGEWQPAQLILIYPMLDATAHFESYIRNGHDYIITRDTLLSGFEMYLPGI
                     ERRHPEASPLWRDDFNGLPPVHIITAEYDPLCDEGEALYHRMTGQGVQCTCQRYLGVI
                     HGFFQLGGISEAARSALRDVAWRAGR"
     gene            complement(2176..3117)
                     /gene="fabY"
                     /locus_tag="ACHKBL_001198"
                     /gene_synonym="yiiD"
     CDS             complement(2176..3117)
                     /gene="fabY"
                     /locus_tag="ACHKBL_001198"
                     /gene_synonym="yiiD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid biosynthesis protein FabY"
                     /protein_id="HFO5818093.1"
                     /translation="MYHLRVPQTEEELERYYQFRWEMLRKPLHQPKGSERDAWDAMAH
                     HQMVVDEQGNLVAVGRLYINADNEASIRFMAVHPDVQDKGLGTLMAMTLESVARQEGV
                     KRVTCSAREDAVEFFAKLGFVNQGEITTPTTTPIRHFLMIKPVATLDDILHRGDWCAQ
                     LQQAWYEHIPLSEKMGVRIQQYTGQKFITTMPETGNQNPHHTLFAGSLFSLATLTGWG
                     LIWLMLRERHLGGTIILADAHIRYSKPISGKPHAVADLGALSGDLDRLARGRKARVQM
                     QVEIFGDETPGAVFEGTYIVLPAKPFGPYEEGGNEEE"
     gene            complement(3162..3599)
                     /gene="dtd"
                     /locus_tag="ACHKBL_001199"
     CDS             complement(3162..3599)
                     /gene="dtd"
                     /locus_tag="ACHKBL_001199"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HFO5818094.1"
                     /translation="MIALIQRVTRASVTVEGEVTGEIGAGLLVLLGVEKDDDEQKANR
                     LCERVLGYRIFSDAEGKMNLNVQQAGGSVLVVSQFTLAADTERGMRPSFSKGASPDCA
                     EALYDYFVERCRQQEMNTQTGRFAADMQVSLVNDGPVTFWLQV"
     gene            complement(3596..4468)
                     /gene="yihY"
                     /locus_tag="ACHKBL_001200"
     CDS             complement(3596..4468)
                     /gene="yihY"
                     /locus_tag="ACHKBL_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
                     /protein_id="HFO5818095.1"
                     /translation="MLKTIQDKARHRTRPLWAWLKLLWQRIDEDNMTTLAGNLAYVSL
                     LSLVPLVAVVFALFAAFPMFSDVSIQLRHFIFANFLPATGDVIQRYIEQFVANSNKMT
                     AVGACGLIVTALLLMYSIDSALNTIWRSKRARPKIYSFAVYWMILTLGPLLAGASLAI
                     SSYLLSLRWASDLNTVIDNVLRIFPLLLSWISFWLLYSIVPTIRVPNRDAIVGAFVAA
                     LLFEAGKKGFALYITMFPSYQLIYGVLAVIPILFVWVYWTWCIVLLGAEITVTLGEYR
                     KLKQAAEQEEDDEP"
     gene            complement(4462..5061)
                     /gene="yihX"
                     /locus_tag="ACHKBL_001201"
     CDS             complement(4462..5061)
                     /gene="yihX"
                     /locus_tag="ACHKBL_001201"
                     /EC_number="3.1.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphatase"
                     /protein_id="HFO5818096.1"
                     /translation="MLYIFDLGNVIVDIDFNRVLGAWSDLTRVPLATLKKSFHMGEAF
                     HQHERGEISDEAFAEALCHEMALPLSYEQFSHGWQAVFVALRPEVIAIMHKLREQGHR
                     VVVLSNTNRLHTTFWPEEYPEIRDAADHIYLSQDLGMRKPEARIYQHVLQAEGFSPDD
                     TVFFDDNADNIEGANQLGITSILVKDKTTIPDYFAKVLC"
     gene            complement(5202..6089)
                     /locus_tag="ACHKBL_001202"
     CDS             complement(5202..6089)
                     /locus_tag="ACHKBL_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HFO5818097.1"
                     /translation="MKTERKNEHYLALQQAFDAPWPGPVGELVTLEKGNIHLQIYPHD
                     GARITSLKAFGSEVLRQWQPQRRAFQYGCFPMVPWAGRLGNATLNAGGQCYSLPANKP
                     PHALHGMACYSTWEIIDKTADSLTLRMPLASPWPWQGEVIQTFLLENDALVLQLEVHS
                     YADTFPASAGWHPWFAKKLTPQNTESLQVLFDADWQEEAGSDELPTGNRISPQVGPWD
                     DCFGFYDGVKVKLLWPGKLAMTMTSSANSLVVFDKQPDATCINPLTQAPNAINLTPEF
                     VTPDKPLVIETRWQFTPES"
     gene            complement(6079..7428)
                     /locus_tag="ACHKBL_001203"
     CDS             complement(6079..7428)
                     /locus_tag="ACHKBL_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO5818098.1"
                     /translation="MSEKLPAPREGLSGKAMRRVVMGSFAGALMEWYDFFIFGTAAGL
                     VFAPLFYPDSDPFIGLIASFATFGVGFLTRPLGGIVFGHFGDKIGRKITLIWTLAIVG
                     CSTFLIGFIPTYQEIGIWAPLVLMVLRLIQGFGLGGEYGGAALMTIESAPESRRGFLG
                     SLPQTAASVGIMLATGIFALCNHFLTSEQFLSWGWRIPFWLSAVMLIVGLFIRLHTEE
                     TLDFQKQKTTNNKEKSVPPLIELFKKHPRNILLALGARLAESVSSNIINAFGIVYISS
                     QLALSRDIPLTGMLIASAIGIFSCPLVGWLSDRIGQKSLYLSGAGFCVLFAFPFFLLL
                     DSKSTLIIWCSMILGYNLGPTMMFAVQPTLFTRMFGTKVRYTGLSFAYQFSAILGGLS
                     PLIASSLLALGGGKPWYVALFLFAVSVLSFVCVWLIEPTDEQETASYRYIREQSHEN"
     gene            complement(7527..8960)
                     /locus_tag="ACHKBL_001204"
     CDS             complement(7527..8960)
                     /locus_tag="ACHKBL_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO5818099.1"
                     /translation="MSQITSPATYSISRPQDVIDIVNKNSAINTSIGVIFIALGGILI
                     DAYQAAMVGFGNKYIAAQFGISPGLAATVNASVLIAALIGGLLANRVINRFGQKRAFI
                     IGMGLCTIGAAAVAIAPSIWWVLVCRVIMGFGLGIDFPLATNAVAELRGSTSKKTGTS
                     VNLWQMAWYVSTTVVYLVLLPLLLSGIAEEQLWRYGIFIGAIFAVIFMILRYFFIGES
                     AMWAARVGRYQEACDILGKRYGVQARVAASGTTEAKFSEKAENKYSGGYGILFNDRYR
                     KRTILGCVVATMQAWQYNAVGVYLPLTLAGIISGGLTGALTGSAVVNALCGVTGGMIG
                     SFILQRLGTRRQSMYGFAVVTLALLSLGALATTNPWLSLGLLGSIIFFHSAGPGGLGM
                     TIATLSYPPAIRPTGVGFARAIMRTGAIAGLIFWPMLWGALKTEAFYWLAIVPFLGFL
                     TCVLINWEPLGANVDAEDAEVLAELKK"
     gene            complement(9030..10040)
                     /locus_tag="ACHKBL_001205"
     CDS             complement(9030..10040)
                     /locus_tag="ACHKBL_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001494590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HFO5818100.1"
                     /translation="MDKITQVLFSDIGKVTTQYVEVPHQQLKPHEVRIAPVFYGICGS
                     DLHVLKGGHPFAKPPVVPGHEIAARVTEVGSDVKNVQPGDHVVVDPIMACMECRACKA
                     GRFNLCEPPQVAGFRAPGFARSQHIVPARNCHVAPASLPLKVLAFAEPAACARHCVNR
                     MPKASLESVLVIGAGTIGLSIVQALRIMGAGKITVIEPDAAKRALALKLGAAEVWAPG
                     ELAADVRFTGAIDVVAAQATLNDACTRVYAGGTVVCMGVPSGPREIPLPMMQRFERDL
                     LNSGMYIPEDFDAVIEWLADGRFDTSELVTDLFAIEDAAAAFERAQQNDSIKVMLQFA
                     PE"
     gene            complement(10073..10960)
                     /locus_tag="ACHKBL_001206"
     CDS             complement(10073..10960)
                     /locus_tag="ACHKBL_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000094541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HFO5818101.1"
                     /translation="MSSHFTPFTLKDISRPGGGFAMLAVDQREAMRLMFAAAGQPKPI
                     ADSVLTDFKVAATRILSPYASAVLADKQFCLEQIVEQGAVANSCGLIVAADLFIPGNG
                     IPVDSVEIDMSVDPHKAREMGAKAMKLLVLWREDEPAEERLAMVDKFVRRCRSAGLVS
                     IIEPVVRPPRRGWDFDRESAIVAAAAELGGTEADLYKAEMPLGGKGDEKTLLAACQQL
                     NDQMKMPWVILSSGVDADIFGRAVSIAMKGGASGFLAGRAVWASVVGAQDPQTMLRDV
                     SVPRLQRLAEIVDEGIAQR"
     gene            complement(10985..11863)
                     /gene="yihU"
                     /locus_tag="ACHKBL_001207"
     CDS             complement(10985..11863)
                     /gene="yihU"
                     /locus_tag="ACHKBL_001207"
                     /EC_number="1.1.1.373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001318159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfolactaldehyde 3-reductase"
                     /protein_id="HFO5818102.1"
                     /translation="MATIAFLGLGQMGSPMANNLLQKGHSLQVFDVNTQAVDALVTQG
                     ATAAQTPAEAAASAEFIITMLPNGDIVRQVLLGEKGVCETVSSDALVIDMSTIHPLQT
                     DALIRELQEKGINMMDAPVGRTSVNAIDGTLLILAGGTDEQIARARPILMCMGNELVE
                     AGGPGMGIRVKLINNYMSIALNALSSEAAVLCESLGLNLDVAIKVMSGTAAGKGHFTT
                     TWPGKVLKGDLSPAFMVDLALKDLRIAVDVARETGAPLNMGIAAESYYAAASQDGKGR
                     QDWSALLNQVRQQAGL"
     gene            12036..12932
                     /locus_tag="ACHKBL_001208"
     CDS             12036..12932
                     /locus_tag="ACHKBL_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000196715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HFO5818103.1"
                     /translation="MTRIACVGITVQDRIYSLPTLPEGGGKYQANHYLEIGGGPAATA
                     AVAIAKLGVEVDFIGRVGDDSCGNTLLAELEGWGVNTAFCRRYPNARSSQSAILVDQH
                     GERIIVNYPSPDLGTDAEWLEAIDFSRYDLILADVRWHSGTEKAFSLARLAGVTTLLD
                     ADMTPQDISPLVALADHAVFSTPGLKRMTGLQSPEEGLFQATTQTAGKVYVTLGSEGS
                     LWIEDGHLCQQEAFSVNVVDTTGAGDVFHGALAVALAEKMPTKEAIRFASAVAAMKCT
                     QPGGRAGIPNREQTESFLSLYA"
     gene            12972..13760
                     /locus_tag="ACHKBL_001209"
     CDS             12972..13760
                     /locus_tag="ACHKBL_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFO5818104.1"
                     /translation="MSIIEVTGNPRHDQLVHLIAERGYMNIEELAQLLDVSTQTVRRD
                     IRKLSEQGLITRHHGGAGRVSSVMNTAFEQRELSLTAEKRAIAEAVADYLPERCTVFI
                     TIGTTVEAVARALLNRRDLRIITNSLRVAQILYKNQDIEVMVPGGTLRAHNGGIIGPG
                     AVDFIEGFRADYLITSIGAIEHDGTLLEFDVNEALVARTMIKHARNTLLVADHTKFTA
                     SAAVSIGNARNVRAFFTDAPPPNSFCQLLSEENVELVVAEQEVS"
     gene            complement(13820..15085)
                     /gene="yihN"
                     /locus_tag="ACHKBL_001210"
     CDS             complement(13820..15085)
                     /gene="yihN"
                     /locus_tag="ACHKBL_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO5818105.1"
                     /translation="MLTKKKWALFSLLTLCGGTIYKLPSLKDAFYIPMQEYFHLTNGQ
                     IGNAMSVNSFVTTVGFFLSIYFADKLPRRYTMSFSLIATGLLGVYLTTMPGYWGILFV
                     WALFGVTCDMMNWPVLLKSVSRLGNSEQQGRLFGFFETGRGIVDTVVAFSALAVFTWF
                     GSGLLGFKAGIWFYSLIVIAVGIIIFFVLNDKEEAPSVEVKKEDGASQNTSMTSVLRD
                     KTIWLIAFNVFFVYAVYCGLTFFIPFLKNIYLLPVALVGAYGIINQYCLKMIGGPIGG
                     MISDKILKSPSKYLCYTFIISTAALVLLIMLPHESMPVYLGMACTLGFGAIVFTQRAV
                     FFAPIGEAKIAENKTGAAMALGSFIGYAPAMFCFSLYGYILDLNPGIIGYKIVFGIMA
                     CFAFCGAVVSVMLVKRISQRKKEMLAAEA"
     gene            complement(15187..16167)
                     /gene="yihM"
                     /locus_tag="ACHKBL_001211"
     CDS             complement(15187..16167)
                     /gene="yihM"
                     /locus_tag="ACHKBL_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HFO5818106.1"
                     /translation="MVTINNARKILQRVDTLPLYLHAYAFHLNMRLERVLPADLLDIA
                     SENNLRGVKIHVLDGERFSLGNMDDKELSAFGDKARRLNLDIHIETSASDKASIDEAV
                     AIALKTGASSVRFYPRYEGNLRDVLSIIANDIAYVRETYQDSGLTFTIEQHEDLKSHE
                     LVSLVKESEMESLSLLFDFANMINANEHPIDALKTMAPHITQVHIKDALIVKEEGGLG
                     HKACISGQGDMPFKALLTHLICLGDDEPQVTAYGLEEEVDYYAPAFRFEDEDDNPWIP
                     YRQMSETPLPENHLLDARLRKEKEDAINQINHVRNVLQQIKQEANHLLNN"
     gene            complement(16175..16885)
                     /gene="yihL"
                     /locus_tag="ACHKBL_001212"
     CDS             complement(16175..16885)
                     /gene="yihL"
                     /locus_tag="ACHKBL_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFO5818107.1"
                     /translation="MAENQSTVENAKEKLDRWLKDGITTPGGKLPSERELGELLGIKR
                     MTLRQALLNLEAESKIFRKDRKGWFVTQPRFNYSPELSASFQRAAIEQGREPSWGFTE
                     KSRTRDIPETLVPLITVTPSTELYRITGWGALEGHKVFYHETYINPEVAPGFIEQLEN
                     HSFSAVWEKCYQKETVVKKLIFKPVRMPGDISKYLGGSAGMPAILIEKHRADQQGNIV
                     QIDIEYWRFEAVDLIINL"
     gene            complement(17102..18925)
                     /gene="typA"
                     /locus_tag="ACHKBL_001213"
     CDS             complement(17102..18925)
                     /gene="typA"
                     /locus_tag="ACHKBL_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-dependent GTPase TypA"
                     /protein_id="HFO5818108.1"
                     /translation="MIEKLRNIAIIAHVDHGKTTLVDKLLQQSGTFDSRAETQERVMD
                     SNDLEKERGITILAKNTAIKWNDYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAF
                     DGPMPQTRFVTKKAFAYGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLDATDEQLDF
                     PIVYASALNGIAGLDHEDMAEDMTPLYQAIVDHVPAPDVDLDGPFQMQISQLDYNSYV
                     GVIGIGRIKRGKVKPNQQVTIIDSEGKTRNAKVGKVLGHLGLERIETDLAEAGDIVAI
                     TGLGELNISDTVCDTQNVEALPALSVDEPTVSMFFCVNTSPFCGKEGKFVTSRQILDR
                     LNKELVHNVALRVEETEDADAFRVSGRGELHLSVLIENMRREGFELAVSRPKVIFREI
                     DGRKQEPYENVTLDVEEQHQGSVMQALGERKGDLKNMNPDGKGRVRLDYVIPSRGLIG
                     FRSEFMTMTSGTGLLYSTFSHYDDVRPGEVGQRQNGVLISNGQGKAVAFALFGLQDRG
                     KLFLGHGAEVYEGQIIGIHSRSNDLTVNCLTGKKLTNMRASGTDEAVVLVPPIRMTLE
                     QALEFIDDDELVEVTPTSIRIRKRHLTENDRRRANRSPKDD"
     gene            19297..20706
                     /gene="glnA"
                     /locus_tag="ACHKBL_001214"
     CDS             19297..20706
                     /gene="glnA"
                     /locus_tag="ACHKBL_001214"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HFO5818109.1"
                     /translation="MSAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFF
                     EEGKMFDGSSIGGWKGINESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYD
                     RDPRSIAKRAEDYLRSTGIADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEGAWN
                     SSTQYEGGNKGHRPAVKGGYFPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAG
                     QNEVATRFNTMTKKADEIQIYKYVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLA
                     KNGVNLFAGDKYAGLSEQALYYIGGVIKHAKAINALANPTTNSYKRLVPGYEAPVMLA
                     YSARNRSASIRIPVVSSPKARRIEVRFPDPAANPYLCFAALLMAGLDGIKNKIHPGEA
                     MDKNLYDLPPEEAKEIPQVAGSLEEALNELDLDREFLKAGGVFTDEAIDAYIALRREE
                     DDRVRMTPHPVEFELYYSV"
     gene            20880..21929
                     /gene="glnL"
                     /locus_tag="ACHKBL_001215"
     CDS             20880..21929
                     /gene="glnL"
                     /locus_tag="ACHKBL_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HFO5818110.1"
                     /translation="MATGTQPDAGQILNSLINSILLIDDNLAIHYANPAAQQLLAQSS
                     RKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNEVTLVIDGRSHILSVTAQRMPDG
                     MILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
                     DPSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRVTESIHKVAERVVTLVSMELPDNV
                     RLIRDYDPSLPELAHDPDQIEQVLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGER
                     YRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIARNLIDQHSGKIEFTS
                     WPGHTEFSVYLPIRK"
     gene            21941..23350
                     /gene="glnG"
                     /locus_tag="ACHKBL_001216"
     CDS             21941..23350
                     /gene="glnG"
                     /locus_tag="ACHKBL_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HFO5818111.1"
                     /translation="MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALA
                     SKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYL
                     PKPFDIDEAVALVERAISHYQEQQQPRNVQLNGPTTDIIGEAPAMQDVFRIIGRLSRS
                     SISVLINGESGTGKELVAHALHRHSPRAKAPFIALNMAAIPKDLIESELFGHEKGAFT
                     GANTIRQGRFEQADGGTLFLDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRI
                     IAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPRLARHFLQVAARELG
                     VEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESTVAE
                     STSQMQPDSWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAAR
                     LLGWGRNTLTRKLKELGME"
     gene            23462..23572
                     /gene="yshB"
                     /locus_tag="ACHKBL_001217"
     CDS             23462..23572
                     /gene="yshB"
                     /locus_tag="ACHKBL_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YshB family small membrane protein"
                     /protein_id="HFO5818112.1"
                     /translation="MLESIINLVSSGAVDSHTPQTAVAAVLCAAMIGLFS"
     gene            complement(23762..25135)
                     /gene="hemN"
                     /locus_tag="ACHKBL_001218"
     CDS             complement(23762..25135)
                     /gene="hemN"
                     /locus_tag="ACHKBL_001218"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HFO5818113.1"
                     /translation="MSVQQIDWDLALIQKYNYSGPRYTSYPTALEFSEDFGEQAFLQA
                     VARYPERPLSLYVHIPFCHKLCYFCGCNKIVTRQQHKADQYLDALEQEIVHRAPLFAG
                     RHVSQLHWGGGTPTYLNKAQISRLMKLLRENFQFNADAEISIEVDPREIELDVLDHLR
                     AEGFNRLSMGVQDFNKEVQRLVNREQDEEFIFALLNHAREIGFTSTNIDLIYGLPKQT
                     PESFAFTLKRVAELNPDRLSVFNYAHLPTIFAAQRKIKDADLPSPQQKLDILQETIAF
                     LTQSGYQFIGMDHFARPDDELAVAQREGVLHRNFQGYTTQGDTDLLGMGVSAISMIGD
                     CYAQNQKELKQYYQQVDEQGNALWRGIALTRDDCIRRDVIKSLICNFRLDYAPIEQQW
                     DLLFADYFAEDLKLLAPLAKDGLVDVDEKGIQVTAKGRLLIRNICMCFDTYLRQKARM
                     QQFSRVI"
     gene            complement(25324..25833)
                     /gene="yihI"
                     /locus_tag="ACHKBL_001219"
     CDS             complement(25324..25833)
                     /gene="yihI"
                     /locus_tag="ACHKBL_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HFO5818114.1"
                     /translation="MKPSSSNSRSKGHAKARRKTREELDQEARDRKRQKKRRGHAPGS
                     RAAGGNTTSGSKGQNAPKDPRIGSKTPIPLGVTEKVTKQHKPKSEKPMLSPQAELELL
                     ETDERLDALLERLEAGETLSAEEQSWVDAKLDRIDELMQKLGLSYDDDEEEEEDEKQE
                     DMMRLLRGN"
     gene            complement(25952..26118)
                     /locus_tag="ACHKBL_001220"
                     /pseudo
     CDS             complement(25952..26118)
                     /locus_tag="ACHKBL_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546704.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            26415..27047
                     /gene="yihA"
                     /locus_tag="ACHKBL_001221"
     CDS             26415..27047
                     /gene="yihA"
                     /locus_tag="ACHKBL_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HFO5818115.1"
                     /translation="MTNLNYQQTHFVMSAPDIRHLPSDTGIEVAFAGRSNAGKSSALN
                     TLTNQKSLARTSKTPGRTQLINLFEVADGKRLVDLPGYGYAEVPEEMKRKWQRALGEY
                     LEKRQSLQGLVVLMDIRHPLKDLDQQMIEWAVDSNIAVLVLLTKADKLASGARKAQLN
                     MVREAVLAFNGDVQVETFSSLKKQGVDKLRQKLDTWFSEMQPVEETQDGE"
     gene            complement(27371..30157)
                     /gene="polA"
                     /locus_tag="ACHKBL_001222"
     CDS             complement(27371..30157)
                     /gene="polA"
                     /locus_tag="ACHKBL_001222"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HFO5818116.1"
                     /translation="MVQIPQNPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLN
                     MLRSLIMQYKPTHAAVVFDAKGKTFRDELFEHYKSHRPPMPDDLRAQIEPLHAMVKAM
                     GLPLLAVSGVEADDVIGTLAREAEKAGRPVLISTGDKDMAQLVTPNITLINTMTNTIL
                     GPEEVVNKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYAEP
                     EKIAGLSFRGAKTMAAKLEQNKEVAYLSYQLATIKTDVELELTCEQLEVQPPVAEELL
                     GLFKKYEFKRWTADVEAGKWLQAKGAKPAAKPQETSVVDEAPEVTATVISYDNYVTIL
                     DEETLKAWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDA
                     PDQISRERALELLKPLLEDEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILN
                     SVAGRHDMDSLAERWLKHKTITFEEIAGKGKNQLTFNQIALEEAGRYAAEDADVTLQL
                     HLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRLAEL
                     EKKAHEIAGEEFNLSSTKQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLP
                     KVILEYRGLAKLKSTYTDKLPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQNIPVR
                     NEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAE
                     VFGLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGV
                     LEYMERTRAQAKEQGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADII
                     KRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHKDDVDAVAKQIHQLMENCTRLDVPL
                     LVEVGSGENWDQAH"
BASE COUNT         7598 a         7880 c         7354 g         7480 t
ORIGIN      
        1 gatctttatt tgcataatct gaatggtgta gccgaaaaat gtcagcatcc taacagcacc
       61 cggatttaag gtgaaattaa tcatccatca cagaccggtc agtactgcta ccaaccggtc
      121 ttaacttgct catcgttata attttgttgg taacagttcg ggaaacaagg aattccttcc
      181 ggaacacccc cggtatcatc cgatagcatt gacgtcaatc atcggcgcga cgatgagttt
      241 ttttaatcgc atcatagggt ggcaacggtc agcacgatta cgggtcatag caaaactcct
      301 gagcaggtgg cactacgccg ctttcacagg agttctgaat gctggtgctt tctgtatggc
      361 ataaccatgc ctctaacggt taagtgcttg cagcacatgt tgcggatcat tggcaatcgc
      421 tcgcagcaaa gcctttgcag gcccggtcgg tgaacgtcgc ccctgctccc agttacgcaa
      481 cgtgtctacg cttacagaaa taagatcggc aaatttagat tgtgacaacc ctgaagcgcg
      541 gcgtatttct ttgattttca tagcatcaac ttcaaaagtc cgcgaaggct gtcgcgttcc
      601 ttcaataatt tcattcatct gggccatgct gtgagtcaac cgctcaaaaa gtgccttatc
      661 catttaccac ctcgtattta acaaccggag cattgctttt tcctggggag acagatcatc
      721 tttaataccc ttacgataaa taagcagtaa tctgatctca taatgtttaa cctgatgaaa
      781 gtaaataacc ctgacaccag cacgtttccc ctttcctcct gacacccatc gaacctttct
      841 taacccacca gtttcaggta ttacctcacc atagtcagga ttcaaggcca ggaaaaactg
      901 aaaacgactg aattcatcgt cggtcagcag tttttgaaca tcttcagtaa aaatttccgt
      961 ttcgatgaag agcataatta tacgccattg gcttagagta acaatccttt ttgtacgtca
     1021 ttgacgtacc cttgaaacca ctagcttact gatagtgctc cagcaaaacc acgtctctgc
     1081 cgggtttgtc tttacatttg cgaggcgctt tccagatttt tcacgcccgt gaatttccct
     1141 gtatccatcc agtaaaaacg ggtgtataaa actgtacatt catacagcaa aatgagttct
     1201 actatggcac aggaaaaagg aattgcagag ttagtcgaag agtttatcgc tgcgggtcgc
     1261 ccctcatcgc gtcaacaaag tattgcgcag cgcagggagg gctatatcgc cagcgctgtc
     1321 cttgcgggtg agacagaaac gcgggtagat attcagacta tcgaacttga agcgatgacc
     1381 ttacgaatag tgtcaccgct gaatgccccg acattactcc cgacgataat ttattactac
     1441 ggtggctgtt ttgttagcgg cgggttcgca actcacgaca accagctgcg ccagttagcc
     1501 tattacgggc aatgtcgggt tattgccgtc cagtatcggc tggcaccgga acatactttc
     1561 cccgccgccc atgatgatgc gcaacgaggt gcagaattag tctggcaaca cgccgaacga
     1621 ctcggcgttg ataaacaacg gataactctt gccggtgaca gtgcgggcgg acatctggcg
     1681 ctggtaactg ctttgcgatt aaagcgtgct ggcgagtggc aaccggcaca actcatctta
     1741 atttatccca tgcttgatgc gactgcgcat ttcgaaagct acatacgcaa tggtcacgat
     1801 tacatcatca cacgcgacac tttactgagc ggctttgaga tgtacctgcc aggcattgaa
     1861 cgcaggcatc ctgaagccag ccctctttgg cgcgatgatt tcaatggctt gccacctgta
     1921 catatcatta ccgctgaata cgacccactt tgcgatgaag gtgaagcgtt gtatcaccgc
     1981 atgacagggc aaggagtgca atgcacctgc caacgctacc ttggcgttat ccacggattt
     2041 tttcagttag gtggtatcag tgaggctgcg cgatcggcgc tacgggatgt tgcctggaga
     2101 gcgggaaggt aactccggac cagggcccag aaaaggtttt cagattgaaa accttttcag
     2161 tcaagtaaaa cgcccctact cttcttcgtt cccgccctct tcatacgggc caaatggctt
     2221 cgcgggcaga acgatatacg tgccttcaaa cactgcaccc ggcgtctcgt cgccaaagat
     2281 ttctacttgc atctgcaccc gtgcttttcg tccgcgcgcc agacggtcga gatcgccgct
     2341 taaggcacca aggtcggcta ccgcatgagg tttgccgcta atcggcttgc tgtagcggat
     2401 atgcgcatcc gccagaataa tcgttccgcc gaggtggcgt tcgcgcagca tcagccagat
     2461 aagcccccaa ccggtgagcg ttgccagcga gaataaactc ccggcaaaca gcgtgtgatg
     2521 cggattctga ttgcctgttt ccggcatggt ggtgataaat ttttgcccgg tatattgctg
     2581 aatgcgcacg cccatttttt cactcagcgg gatgtgttca taccacgcct gttgcagctg
     2641 cgcgcaccag tcgccgcgat gcaaaatatc atccagagtg gcaacgggtt taatcatcaa
     2701 aaaatggcga atcggcgtgg tggttggcgt ggtgatttct ccctgattaa caaaccccag
     2761 cttggcgaaa aactccaccg cgtcttcgcg ggcgctacag gtcacgcgct taacaccttc
     2821 ctgacgcgcc acagattcga gggtcatcgc catcagcgtt cctaaccctt tgtcctgcac
     2881 gtcgggatga acggccataa agcgaatgga cgcttcattg tcggcattaa tatacagtcg
     2941 gcctaccgcc accagattac cctgctcgtc gacgaccatc tggtgatgcg ccatcgcatc
     3001 ccacgcgtcg cgttccgaac cttttggttg atgcaggggc ttacgcaaca tttcccagcg
     3061 aaactgatag taacgctcta attcttcttc tgtttgtgga acccgaaggt gatacatagc
     3121 gatactctct cttgtttccc gtgacaaccc tggaagctgg ctcatacctg caaccagaat
     3181 gtcacggggc catcattgac cagcgatacc tgcatatccg cagcgaagcg tcctgtttgc
     3241 gtgttcatct cttgctgacg gcagcgttcg acgaaatagt catataacgc ctctgcgcaa
     3301 tccggtgatg cgcctttgga gaaacttggg cgcatccccc gttcggtatc tgcggcaagg
     3361 gtaaactggg aaaccaccag tacactgccg cccgcctgtt gcacgttgag attcatcttg
     3421 ccttcggcat cgctaaagat gcggtagccg agcacacgct cgcacagacg gtttgctttc
     3481 tgttcgtcgt catccttttc gacacccaat aacaccaaaa gtcccgcgcc aatttcaccc
     3541 gtcacttctc cctccacggt gacgctggca cgggttacgc gttgaattaa tgcaatcatg
     3601 gttcgtcgtc ttcttcttgt tcagcagctt gttttaattt gcggtattcc ccgagagtga
     3661 cagtaatttc cgcgccaagc aagacgatac accatgtcca gtagacccaa acaaagagaa
     3721 tggggatcac cgccagcaca ccgtaaatga gctgatatga tgggaacatg gtgatataaa
     3781 gcgcgaaacc tttctttcct gcttcgaaca ggagcgcggc gacaaacgcg ccgacaatcg
     3841 cgtcgcggtt aggtacacgg atggtaggaa cgatgctgta cagcagccag aaagagatcc
     3901 acgacaacag cagcggaaaa atacgcaaca cattatcgat gacagtattg agatcgctcg
     3961 cccagcgcag agagagcaga taggagctga tcgccagact ggcccctgcc aacagcggcc
     4021 ccagcgttaa aatcatccag tacacggcaa acgagtaaat ttttggtcgc gctcgtttac
     4081 tgcgccagat ggtattcaac gcgctatcga tggagtacat caacaataac gccgtgacga
     4141 tcagcccgca cgccccgacg gcggtcattt tgttggaatt ggcgacaaac tgttcgatat
     4201 atcgctggat aacatcgcca gtagcaggca gaaagttggc aaaaataaag tgacgcaact
     4261 gaatgctgac gtcggaaaac atgggaaaag cggcgaaaag cgcaaaaaca acggcaacca
     4321 gcggcactaa tgagagcaac gacacatagg caaggttacc tgccagggtt gtcatgttgt
     4381 cctcatcaat gcgttgccag agtagtttta gccaggccca tagtggacgg gtacgatgcc
     4441 tggctttgtc ctgaatggtt tttagcataa caccttcgcg aaatagtccg ggatggtggt
     4501 tttatctttc accagaatac tagtaatgcc tagctgatta gctccttcta tattatcggc
     4561 gttatcgtcg aaaaagaccg tatcgtctgg tgaaaaacct tctgcctgca aaacatgctg
     4621 ataaattcgt gcttcaggtt tgcgcatacc caagtcttgc gacagataga tatggtcagc
     4681 agcatcacgg atttccgggt attcctccgg ccagaaggta gtatgcaggc ggttagtatt
     4741 ggaaagcacc accacgcgat gcccctgctc acgcagttta tgcatgatgg cgatcacttc
     4801 cgggcgcagc gcaacaaata ccgcctgcca accgtgggag aactgctcat agcttagcgg
     4861 cagggccatc tcatggcaca gtgcctctgc gaacgcttcg tcgctaattt ccccacgctc
     4921 atgctgatga aacgcctcgc ccatgtggaa actcttctta agcgtagcca gtggaacacg
     4981 cgttaaatcg ctccaggccc ccagcacacg gttaaagtcg atatcgacaa tcacattacc
     5041 taaatcaaag atatagagca tttttgcctc ctttcgcgcc acaagaaatt aactgtagcg
     5101 ggaaaggatg ggtttggcta tgtggcaata agggtaatct tgtgatgctg attgcgaaaa
     5161 agcagagtaa tttcactggc tttttcgcaa tagatgaatg gttaagattc aggtgtaaat
     5221 tgccaacgag tttcgatgac cagcggctta tctggtgtca caaattccgg cgttagattt
     5281 attgcgttag gtgcctgagt cagcgggtta atacaggtgg catcgggctg tttatcaaat
     5341 accaccagac tgtttgcaga agaggtcatg gtcatcgcga gtttaccagg ccacagtaac
     5401 ttaaccttca ccccgtcata aaaaccaaaa cagtcatccc agggaccgac ttgaggagag
     5461 atacgatttc cagttggcag ttcatcacta cccgcttctt cttgccagtc cgcatcaaaa
     5521 agcacctgca acgattctgt attttgcgga gtcagctttt tcgcaaacca cggatgccag
     5581 cctgcggagg ccgggaatgt atcggcatat gaatggactt ccagttgcaa aaccagcgca
     5641 tcgttctcca gcaaaaaagt ctgaatcact tccccttgcc agggccacgg tgaagccagg
     5701 ggcatacgta aagtcaggga atctgctgtt ttgtcgatga tttcccacgt tgagtaacaa
     5761 gccataccat gcaacgcatg tggtggttta ttggcgggta aagaataaca ttgcccacca
     5821 gcattcagcg tcgcattccc cagtcgccct gcccacggca ccattgggaa acagccatac
     5881 tgaaaagctc tgcgctgcgg ctgccattgg cgtaatactt cagaaccaaa tgcctttaac
     5941 gaggttatcc tggcaccatc atgcggataa atctgcaggt gaatattgcc tttttccagc
     6001 gtcactaact ctcctacagg cccaggccat ggcgcgtcaa acgcctgttg tagcgccagg
     6061 taatgctcgt tttttcgttc agttttcatg agattgttcc ctgatgtagc ggtaagaagc
     6121 ggtttcttgt tcatcggttg gctcaatcag ccagacgcaa acaaaggaaa gaactgagac
     6181 agcaaataaa aacagagcga cataccacgg ttttcctccc cctagcgcca gcaaacttga
     6241 ggcgataagg gggcttagtc cgccaagaat cgcggaaaat tgataagcaa aagaaaggcc
     6301 ggtatatcgt actttggtac cgaacatgcg ggtgaaaagt gttggctgca cggcaaacat
     6361 catcgtcgga cctaagttgt agccaaggat catactgcac cagatgatga gcgtactttt
     6421 gctatccagt aataagaaga aaggaaacgc gaacaggaca caaaatcctg ctcccgacaa
     6481 atacagactt ttttgaccta tacgatctga taaccatcca accaatggac aactgaaaat
     6541 accgatcgcc gaggcaatca acatacccgt caggggaata tctcgcgaca atgctaattg
     6601 gctggaaata tagacaatac caaaggcgtt aataatatta gaggagacac tttccgccag
     6661 cctcgcaccc agtgccaata aaatatttcg tggatgtttt ttgaataatt caatcaacgg
     6721 aggaacggac ttttctttat tattcgtcgt tttttgcttt tgaaaatcca gcgtttcttc
     6781 ggtatgcagg cggataaaca gcccaacgat caacatcacc gcagaaagcc aaaacggaat
     6841 gcgccatccc caggagagaa actgctccga agtaagaaaa tggttacaga gagcaaaaat
     6901 accggttgcc agcataatac ccacagacgc agccgtttgc gggagtgaac ctaaaaatcc
     6961 gcgtcgggat tcgggcgctg attctatggt catcaatgcc gctccgccgt actcgccacc
     7021 aagaccaaag ccctgaatca aacgcagcac cattaataca agcggtgccc aaatgccaat
     7081 ttcctgatac gtcggaataa aaccaatcaa aaatgtggag caaccgacaa tagccagcgt
     7141 ccagataagt gttatttttc gcccgatttt atcgccaaaa tgaccaaaga cgatgccacc
     7201 taaaggacga gtcagaaaac caacaccaaa ggtcgcaaag gaagctataa gacctataaa
     7261 aggatcgctg tctggataaa acagcggtgc aaataccagt cctgctgccg tgccaaaaat
     7321 aaagaagtca taccattcca ttaacgcgcc tgcgaaactg cccataacga cacgtctcat
     7381 ggctttaccg gaaagccctt cacgcggtgc gggtaacttt tcagacataa ttttcctctc
     7441 ctggtaggcg agaaatctgg gatacgaacc actaaacgtg ggcagtttga attaatgcgc
     7501 atcggcttaa accgatgcgc attaaattat ttcttcaatt cagccagaac ctctgcgtcc
     7561 tcagcatcaa catttgcacc cagtggttcc caattaatca atacacaggt caggaatccc
     7621 aggaatggca cgattgccaa ccagtaaaac gcttcagttt tcaacgcacc ccacagcatc
     7681 ggccagaaaa tgagtcctgc aattgcccct gtgcgcataa tagcgcgggc aaatccgacc
     7741 ccagtagggc gaatagcggg aggataagag agtgtggcaa tggtcatgcc cagcccacca
     7801 ggacccgctg aatggaagaa aataattgat cccaataacc ctaaagacag ccagggatta
     7861 gtcgttgcca gtgcgcctaa cgacagcaat gctaaggtca caacagcaaa tccatacatc
     7921 gactgccggc gagtacccag tcgttggaga ataaacgagc cgatcatccc gcctgtcacc
     7981 ccacaaaggg cattcacgac ggcagaaccg gttaacgcgc cagtaagccc gccacttatt
     8041 attcccgcca acgtaagagg aagataaaca cctacggcgt tatattgcca cgcctgcatg
     8101 gttgccacga cacagccaag aatggtgcgt ttgcggtaac gatcattaaa taagatgcca
     8161 tatccaccac tgtatttatt ctctgctttt tccgagaatt tcgcttctgt tgtacccgat
     8221 gccgcaacgc gagcctgaac accataacgt tttcccagaa tgtcgcacgc ttcctggtaa
     8281 cgcccgacgc gtgcggccca cattgcggat tcaccaataa agaagtaacg caaaatcatg
     8341 aagatgactg caaaaatagc tccgatgaat attccgtaac gccacaattg ttcttcagcg
     8401 atacccgaca gaagcagcgg caaaagcacc aaataaacaa cggttgtcga aacataccag
     8461 gccatttgcc agaggttgac cgacgtgccg gttttcttcg acgttgaacc acgaagctct
     8521 gccacggcat tggttgccaa agggaagtcg atgcctaaac caaagcccat gatgacgcgg
     8581 cacaccagca cccaccagat actgggagca atagctaccg ctgcagcacc gatggtgcac
     8641 agccccatgc caataataaa tgcccgcttt tgcccaaagc ggtttattac tcggttcgct
     8701 aataaaccgc caattaacgc ggcgattaat actgatgcat tgacggttgc agcaagaccc
     8761 ggcgaaatgc cgaattgagc ggcaatgtat ttattaccaa accccaccat cgccgcctgg
     8821 taggcatcaa tcaatatgcc gcccaaagca ataaaaataa cgccgatact ggtgttgatt
     8881 gcagagttct tattaacaat atcaattacg tcctgtggcc gactaattga ataagttgca
     8941 ggactggtga tttgactcat cgcagtgtcc tttttaattg ttgtagggtg gcgctgacgt
     9001 gacgcagcgc cagacagaga tttagaaaat cattccggcg caaattgcag catgaccttt
     9061 atggagtcat tttgctgcgc gcgttcgaat gccgccgctg catcctcaat ggcaaataaa
     9121 tcggtaacca gttcactggt atcaaaccgc ccatccgcca gccattcgat aacagcatcg
     9181 aaatcttcag ggatgtacat gccagagttg agcaagtcac gctcgaaacg ttgcatcatc
     9241 ggtaatggta tttcacgcgg cccacttggt acgcccatgc acacgacggt gcctccggca
     9301 tatacacggg tacatgcatc gttaagcgtg gcctgcgctg caacgacatc aatcgccccc
     9361 gtaaatcgca catctgcggc cagctcacct ggtgcccaaa cttctgctgc gcccagtttt
     9421 aacgccagcg cgcgtttggc agcgtcaggt tcaatcacgg taatcttacc tgcccccata
     9481 atgcgcagtg cctgcacgat ggataagcct atcgttccgg caccaatcac cagtacgctt
     9541 tccagagaag ctttcggcat tcggttaacg cagtgacgag cacaagccgc cggttcggca
     9601 aaggccaaca cttttagcgg taaagaggct ggtgcgacat ggcaattacg cgcaggaaca
     9661 atgtgttgtg agcgagcaaa gcccggtgcg cggaaaccag caacctgcgg tggttcacaa
     9721 agattaaaac gtcctgcttt gcaggctcgg cattccatgc aagccatgat gggatcgacc
     9781 acaacatgat cgcccggctg tacattttta acgtcgctgc ccacttccgt aacgcgcgct
     9841 gcaatttcat gaccggggac gacaggcggt ttggcaaacg gatgaccgcc tttcagaaca
     9901 tgcagatccg aaccgcatat cccgtagaac acgggcgcaa tccgtacttc gtgcggttta
     9961 agttgctggt gtggtacttc aacatattga gtggtgactt tcccaatatc tgaaaataaa
    10021 acctgggtaa ttttatccat tgttattctc cagaatcgat atccataggg gattagcgtt
    10081 gcgcgatacc ttcatcgaca atttcagcca gccgttgcaa gcgtggaacg gagacatcgc
    10141 gcagcatggt ttgtggatct tgtgcaccaa caaccgacgc ccaaaccgct cgtccagcca
    10201 ggaagcccga cgcaccacct ttcatggcaa tacttactgc acggccaaaa atatcggcat
    10261 caacgccaga agaaagaatt acccacggca tcttcatctg atcgttaagt tgctgacagg
    10321 cagcaagtaa ggttttttca tcgcctttgc cccctaatgg catctcggct ttgtaaagat
    10381 cagcctctgt tccaccaagc tctgcggcgg cggcaacaat ggcgctttca cgatcaaagt
    10441 cccaaccgcg acgaggtgga cgcaccacag gttcaataat gctaaccagc ccggcactac
    10501 ggcagcgacg aacaaattta tcaaccattg ccagacgttc ctctgcgggt tcatcttcac
    10561 gccagagtac aagtaatttc attgccttag cccccatttc acgggcttta tgcgggtcga
    10621 cggacatatc tatttcaacg ctatcaaccg ggatgccatt acctgggata aaaagatcgg
    10681 cagcgacaat caggccgcaa gaattagcca cagcgccctg ctcgactatt tgttcaagac
    10741 aaaattgttt atccgccaga acggcagaag cataaggaga aaggattcgg gtcgcggcaa
    10801 ctttaaaatc ggtgagtaca ctgtcggcga tgggttttgg ctgccctgct gccgcaaaca
    10861 tcaggcgcat ggcctcacgc tgatcgacag ctaacatggc gaatccacca cctgggcggg
    10921 agatatcttt cagcgtaaat ggggtgaagt gggatgacat agaaactcct gttaatagat
    10981 aaaattaaag accagcctgc tggcgaacct gatttagcag ggcgctccag tcctgccgcc
    11041 ctttgccgtc ttgacttgct gcggcgtaat aactttctgc tgcaatcccc atattgagcg
    11101 gcgcccccgt ttcccgagcg acgtcaactg caatacgcag atctttcaat gccaggtcca
    11161 ccatgaaggc gggagaaaga tcgcctttca gcactttgcc aggccaggtg gtcgtaaaat
    11221 gtcctttccc tgctgccgta ccactcatca ccttaattgc gacatcaaga tttaatccca
    11281 gtgactcaca taacacagcg gcttcggagg acagcgcatt aagcgcaatg ctcatatagt
    11341 tgttaattag cttcacgcgg atccccattc ctgggccacc agcctcgacc agctcattac
    11401 ccatgcacat gagaatcggg cgtgcacgcg caatctgctc atcagtgccg cccgctaaaa
    11461 tcagcaatgt gccatcaatc gcattcactg acgttctgcc cacgggggcg tccatcatat
    11521 tgatgccttt ttcttgtaat tcccgtatta atgcatcggt ctgtaaggga tgaatagtcg
    11581 acatatcaat cactaacgcg tcagatgaaa cagtttcaca gacgcctttt tcccccaaca
    11641 gcacctgtcg aacaatgtcc ccattgggaa gcatagtgat gataaattca gcgcttgctg
    11701 ccgcttccgc tggggtttgt gccgcagtgg ccccctgcgt aactagcgca tcgacggcct
    11761 gtgtattgac atcaaatacc tgtaatgaat gccctttttg caatagattg ttagccattg
    11821 ggctacccat ctgccccagc ccgagaaatg caatggttgc catgaatact ctcctgtctg
    11881 acattgacaa aataatgaca attgaaaatt cgttttcgtc tgtttttgag catatttgta
    11941 atttatgttc aaaaacacga cacgcatcac taatttgatc atttgtgtca tttaaattga
    12001 ccaaagacga caaaattaaa caggacaaga caaatatgac cagaattgca tgcgtgggta
    12061 tcacagttca ggacaggatt tattctttgc ccactctgcc ggagggcggc gggaaatatc
    12121 aggcaaatca ttatcttgag attggtggcg gcccggcagc aacagccgct gttgcaatcg
    12181 caaagcttgg cgtggaggtt gatttcatcg gtcgcgtcgg cgatgacagt tgtggaaata
    12241 ccttacttgc agaacttgag ggttggggcg tcaataccgc attttgccgc cgatatccta
    12301 acgcccgttc gtcgcaatca gcgatcctcg ttgatcagca tggcgaacgg attatcgtta
    12361 attatccaag tcctgatctc ggtactgatg ccgaatggtt agaagcgatc gatttctctc
    12421 gttatgatct gattctggcc gatgttcgct ggcatagcgg tactgaaaaa gcattctcgc
    12481 tagcgcgcct tgcgggtgtg acaacgcttt tagacgccga tatgacaccg caggatattt
    12541 ctccacttgt cgcgcttgcc gatcacgccg tgttctcaac acctgggctg aaacgcatga
    12601 ctggcctgca gtcacctgaa gaaggtttgt tccaggcaac gacacaaaca gcaggtaaag
    12661 tgtatgttac gctgggttct gaaggatcgt tgtggattga agatggtcat ttgtgtcaac
    12721 aggaggcatt ttctgtcaat gtggttgata ccactggagc aggcgatgtt tttcacggcg
    12781 cactcgcggt agcgttggca gaaaaaatgc caacaaaaga ggctattcgt tttgccagtg
    12841 cggttgccgc gatgaaatgt acccagcctg gcggtcgggc cggcattcct aatcgtgagc
    12901 aaaccgaatc atttttgtca ctttatgcgt aaaatggtaa acatctaccg aggaggggca
    12961 agggagtatt catgagtatc atcgaagtga caggtaatcc acgccatgac caactcgttc
    13021 atctaatcgc agaacgcggt tatatgaata ttgaagaact ggcacaactg ctggatgttt
    13081 caacgcaaac cgtgcgccgg gatatccgaa aactcagtga gcagggcttg atcacccgtc
    13141 atcatggagg ggccgggcgc gtctccagcg tcatgaatac cgcttttgag caacgggaac
    13201 tttcgctcac cgccgagaaa cgggcgatcg ctgaggcagt cgccgattac cttcccgaac
    13261 gctgtaccgt ctttatcacc atcggtacaa ccgtagaagc cgttgccagg gcattactca
    13321 accggcgtga tttacgcatt atcactaata gcctgcgtgt ggcacagatt ctttataaga
    13381 atcaggatat tgaagtgatg gtgccgggag gcactttacg cgctcataac ggtgggatta
    13441 tcggcccagg agccgtggat tttattgaag gcttccgcgc agattattta atcaccagta
    13501 taggtgccat tgaacatgac ggcaccctac tggaatttga tgtcaatgaa gcgttagtcg
    13561 cgagaacgat gattaaacat gcgcggaata cattgttagt ggccgatcat acgaagttta
    13621 ccgcgtctgc tgccgtttca attggcaatg cacggaatgt cagggctttt tttactgatg
    13681 ccccgcctcc caattctttc tgccagttgt taagtgaaga gaatgttgaa ctggtggttg
    13741 ccgagcaaga agtatcctga aattgaaact tcggttgata cggttttcgt tggccggatt
    13801 aagcacatcc ggccactaat taagcttcag ccgccagcat ctctttctta cgttggctaa
    13861 tacgcttaac cagcattacg gaaaccaccg caccacagaa tgcgaagcag gccataatgc
    13921 caaacacgat tttgtagcca ataatccccg gatttaaatc cagaatgtag ccatacagac
    13981 tgaagcagaa catcgccggg gcgtaaccaa taaagctacc caacgccatc gccgcgcctg
    14041 ttttattttc agcaattttt gcttcgccga taggtgcaaa aaatacggct cgctgtgtga
    14101 agactatagc gccaaagccc agcgtacatg ccatccctaa atagaccggc atactttcgt
    14161 gcggcagcat aatcaacagt acgagcgcag cggtacttat gataaaggtg tagcatagat
    14221 atttactcgg cgatttcagg atcttatctg aaatcatgcc accaatcggt cctccaatca
    14281 ttttcagaca gtattggtta atgatgccgt aagcccccac cagagcaacg ggcaatagat
    14341 agatgttttt caggaatgga atgaagaatg ttaggccaca gtaaaccgcg taaacgaaga
    14401 agacgttgaa agcgataagc cagatggttt tatctctcag caccgaggtc atactggtgt
    14461 tttgcgatgc tccgtcttct tttttcacct caacggacgg tgcctcttct ttgtcattca
    14521 ggacaaagaa aatgataatg cctacggcaa tcacaataag ggaatagaac cagatgcctg
    14581 ctttaaaacc caataagcca ctgccaaacc aggtaaatac tgccaacgca gaaaatgcca
    14641 ccacggtatc gacaatgcca cgccctgttt cgaagaagcc aaacaaccga ccttgttgtt
    14701 cgctattgcc caatcgactt accgacttga gcaagaccgg ccagttcatc atgtcgcaag
    14761 taacgccaaa tagcgcccag acaaagagga tgccccaata ccccggcatt gtcgtcaaat
    14821 aaacacccag taatcctgtc gcaatgagtg agaatgacat ggtgtatctg cgcggtagtt
    14881 tatcggcaaa ataaatagac agaaaaaagc ccactgtggt gacaaatgag tttaccgaca
    14941 tagcattacc aatttgacca ttggtcaaat ggaaatattc ctgcatcggg atataaaacg
    15001 catctttcag agacggtaat ttataaattg taccgccaca caatgttaat agactaaata
    15061 acgcccattt ctttttcgtg agcataacat cccctaagcg atttttaaaa tcactgtttt
    15121 aaaggcaata agataccgac cattcatatg gaatggtttt attaacagcg tatgagttaa
    15181 aacttattag ttgttcagaa gatggtttgc ctcttgtttg atttgttgta gtacattacg
    15241 cacatgattt atctgattaa ttgcatcttc tttttcttta cgtaaccgcg catccagtaa
    15301 atgattttct ggcaatggtg tttcactcat ctggcgataa gggatccacg gattatcatc
    15361 ttcgtcttca aagcggaacg ccggcgcata gtaatcaacc tcttcttcca ggccatatgc
    15421 cgtcacctgc ggctcatcat cacccaggca gataaggtgc gttaataacg ctttgaacgg
    15481 catatcaccc tgacctgaaa tacaggcttt atgacccagg ccaccctctt ctttaacgat
    15541 caaggcatct ttgatatgga cctgggtaat atgcggtgcc atcgttttta aagcgtcgat
    15601 gggatgctca tttgcattga tcatgttcgc aaaatcaaac agtaaggaaa gagattccat
    15661 ctcactttct ttgaccagcg acaccagctc atggcttttt aaatcttcat gctgttcgat
    15721 cgtaaaagtc aggccgctgt cctgatacgt ttcccgtaca taggcaatgt cgttagcgat
    15781 gatcgataat acgtcgcgca aattaccttc ataacgtgga taaaaacgta cggacgatgc
    15841 cccagttttc aacgcaatgg cgacggcttc gtcaatagat gccttatctg aagcgctggt
    15901 ttcaatatga atatcaaggt tcagacggcg ggctttatcc ccaaaggcag agagttcttt
    15961 atcgtccata ttaccaagag aaaaacgctc gccatccaga acatggattt tgacgccacg
    16021 tagattattt tcacttgcga tatcaagtaa atcagcaggc aacactcttt ccagccgcat
    16081 atttaaatga aaggcataag catgtaaata aagaggaaga gtgtcgacac gttgtagaat
    16141 ctttcttgcg ttatttattg tcaccataaa acacctacag attaatgatg aggtctacgg
    16201 cctcaaatcg ccaatattca atatctatct ggacaatatt gccttgctgg tcggcgcgat
    16261 gcttttcaat taagatcgct ggcatacccg cagaaccgcc aagatacttg ctgatatcgc
    16321 ccggcattct gacgggtttg aaaatcaatt tttttactac cgtctctttt tggtagcact
    16381 tttcccagac tgcagaaaat gagtggtttt caagttgttc aataaaaccc ggggcaactt
    16441 caggattaat atatgtttcg tgatagaaaa ctttatgtcc ttccagcgct ccccagccgg
    16501 tgatgcgata gagttcagtt gatggtgtca ctgtaatcag tggcacgagc gtctcgggaa
    16561 tatctctggt acggcttttc tcggtaaacc cccaggaagg ctcccgccct tgctcaattg
    16621 cagcccgctg aaagctcgcc gacagctctg gactgtaatt aaatcgcggc tgggtcacga
    16681 accacccttt acgatcctta cggaagattt tggattctgc ctcgaggttc aacaacgcct
    16741 ggcgcagcgt catacgttta atgcccagca gttctcccag ctctctttct gaagggagtt
    16801 ttccacccgg cgtggtgatg ccatctttca accaccgatc cagtttctct tttgcatttt
    16861 ctacggtgga ttgattctcc gccattgttt aatccttttg gtttaaacca gatgaggcat
    16921 cttacgctta cgccaaaaaa gaaagcaatt aacggcgtat gactttgcag ataattgtga
    16981 caaagctctc tattgacgta atcacagact tataagggag atactcgcat tggtgtgcgc
    17041 tcacgacagg taaaaaaaaa cctgccagcg atggcaggtt ttttatgact gaaaagcgaa
    17101 attaatcgtc tttcggtgag cggttggcgc gacgacgatc gttttccgtc aggtgacgtt
    17161 tacgaatacg gatagaggtc ggagtcactt ctaccagttc gtcatcatcg atgaactcca
    17221 gagcttgttc cagagtcatg cggataggcg gaaccagaac aacggcttcg tcagtaccgg
    17281 aagcacgcat gttggtcagt ttcttaccgg tcaggcagtt tacagtcagg tcgttagagc
    17341 ggctatgaat accgataatc tgaccttcgt aaacttctgc accgtgaccg aggaacagct
    17401 taccgcgatc ctgcagaccg aacagcgcga acgcgaccgc tttaccctga ccgttagaga
    17461 tcagtacacc gttctgacgc tgacccactt cgcccggacg tacgtcgtcg tagtggctga
    17521 aggtggagta cagcagacca gtaccggaag tcatggtcat gaactcagaa cggaagccaa
    17581 tcagaccacg gcttgggatc acgtagtcga gacgtacgcg gcctttaccg tctggattca
    17641 tgtttttcag gtcgccttta cgttcgccca gcgcctgcat cacagaaccc tgatgctgtt
    17701 cttcaacgtc cagcgtcacg ttttcatacg gctcttgttt acgaccgtcg atttcacgga
    17761 agattacttt cggacgggat accgccagtt cgaaaccttc acgacgcatg ttttcgataa
    17821 gaacagacag gtgcagttcg ccacgaccag aaacgcggaa cgcatcggcg tcttcggttt
    17881 cttctacgcg cagcgcaacg ttgtgtacca gttctttgtt cagacgatcc aggatctgac
    17941 gagacgttac gaacttacct tctttaccgc agaacggcga ggtgttaacg cagaagaaca
    18001 tagaaacggt cggctcatca acggagagtg ccggcagcgc ttcaacgttt tgcgtgtcgc
    18061 aaacggtgtc agaaatgttc agttcgccca ggcccgtgat cgccacgata tcgccagctt
    18121 ccgccagatc ggtttcgata cgttccagac cgaggtggcc cagcacttta ccgactttcg
    18181 cgttgcgggt tttgccttcg ctatcgatga tagtaacctg ctggttcggc ttcactttac
    18241 cgcgcttgat gcggccaatg ccgataacgc caacatagct gttgtaatcg agctgagaaa
    18301 tctgcatctg gaacggaccg tcaaggtcaa cgtccggcgc aggaacgtgg tcaacaatcg
    18361 cctgatacag cggggtcatg tcttccgcca tatcttcgtg gtccagaccc gcgataccgt
    18421 tcagcgcaga agcgtaaacg atcgggaagt ccagctgctc gtcggtcgcg tcgaggttaa
    18481 cgaacagatc gaatacctga tccacaaccc aatcaggacg cgcgccaggg cggtcaactt
    18541 tgttgataac aacaatcggc ttcaggccgt aagcaaacgc ttttttggtt acgaagcgcg
    18601 tttgcggcat cgggccgtca aatgcgtcaa ccaccagcag cactgagtct accatggaca
    18661 ttacacgttc aacttcacca ccgaagtcgg cgtgccccgg ggtatcaacg atgttgatac
    18721 ggtaatcatt ccatttgata gcggtgtttt tcgcgaggat ggtaatccca cgctctttct
    18781 ccaaatcgtt ggagtccatc acgcgctctt gggtttcggc acgagagtcg aacgtaccgg
    18841 attgttggag cagcttgtct accagggtgg ttttaccatg gtctacgtgc gcgatgatgg
    18901 cgatattacg caatttttcg atcacaactt tgcctcaggc attagaaata gcgcgttatt
    18961 gtacacggat taatcgcact acaaaacagg atcacaaaca tcctccgcaa acaagtattg
    19021 cagagtccct ttgtgatcgc tttcacggag cataaaaagg gttatccaaa ggtcattgca
    19081 ccaatatggt gcttaatgtt tccattgaag cactatattg gtgcaacatt cacatcatgg
    19141 tgcagccctt ttgcacgatg ttaagcatga taacgccttt taggggcaat ttaaaagttg
    19201 gcacagtttt cgctttatct ttttacggcg acacggccaa aataattgca gatttcgtta
    19261 ccacgacgac catgaccaat ccaggagagt taaagtatgt ccgctgaaca cgtactgacg
    19321 atgctgaacg agcacgaagt gaagtttgtt gatttgcgct tcaccgatac caaaggtaaa
    19381 gaacagcacg tcactatccc tgctcatcag gtgaatgctg aattcttcga agaaggcaaa
    19441 atgtttgacg gctcctcgat tggcggctgg aaaggcatta acgaatccga catggtgctg
    19501 atgccagacg catccaccgc agtgattgac ccgttcttcg ccgattctac cctgattatc
    19561 cgttgcgaca tccttgaacc tggcaccctg caaggctatg accgtgaccc gcgctctatt
    19621 gcgaagcgcg ccgaagatta cctgcgttcc actggcattg ccgacaccgt actgttcggg
    19681 ccagaacctg aattcttcct gttcgatgac atccgtttcg gatcatctat ctccggttct
    19741 cacgttgcta tcgacgatat cgaaggcgca tggaactcct ccacccaata cgaaggtggt
    19801 aacaaaggtc accgtccggc agtgaaaggc ggttacttcc cggttccgcc ggtagactct
    19861 gctcaggata ttcgttctga aatgtgtctg gtgatggaac agatgggtct ggtggttgaa
    19921 gcccatcacc acgaagtagc gactgctggt cagaacgaag tggctacccg cttcaatacc
    19981 atgaccaaaa aagctgacga aattcagatc tacaaatatg ttgtgcacaa cgtagcgcac
    20041 cgcttcggta aaaccgcgac ctttatgcca aaaccgatgt tcggtgataa cggctccggt
    20101 atgcactgcc atatgtcgct ggctaaaaat ggcgttaacc tgttcgcggg cgacaaatat
    20161 gcaggtctgt ctgaacaggc gctgtactac attggcggcg tcatcaaaca cgcgaaagcg
    20221 attaacgccc tggcaaaccc gaccaccaac tcttacaagc gtctggtccc gggctacgaa
    20281 gcaccggtaa tgctggctta ctctgcgcgt aaccgttctg cgtctatccg tattccagtg
    20341 gtttcttctc cgaaagcacg tcgtatcgaa gtacgtttcc cggacccggc ggctaacccg
    20401 tacctgtgct ttgctgccct gctgatggcc ggtcttgacg gtatcaagaa caagatccat
    20461 ccgggcgaag cgatggacaa aaacctgtat gacctgccgc cggaagaagc gaaagagatc
    20521 ccacaggttg ctggttctct ggaagaagca ctgaacgaac tggatctgga ccgtgagttc
    20581 ctgaaagccg gtggcgtgtt cactgatgaa gcgatcgatg cgtacatcgc tctgcgtcgc
    20641 gaagaagatg accgcgtgcg tatgactccg cacccggtag agtttgagct gtactacagc
    20701 gtctaatagt tgaagttgta ctacccggcg caacaacgcc gggatttagt tgccgtggaa
    20761 actttcagcc catctctgca tgggcttttt tctccgtcaa ttctctgatg cttcgcgctt
    20821 tttatccgta aaaagctata atgcactaaa atggtgcaac ctgttcagga gactgcttta
    20881 tggcaacagg cacgcagccc gatgctgggc agatcctcaa ctcgctgatt aacagtattt
    20941 tgttaatcga tgacaacctg gcgatccatt acgccaaccc cgccgcgcaa caactgctcg
    21001 cccaaagctc ccgcaaattg tttggtacac cgttaccgga actgttgagc tacttctcat
    21061 taaatatcga gctgatgcaa gaaagtctgg aggcggggca aggttttact gataacgaag
    21121 tgacgctggt catcgacggg cgctcgcata tcctttctgt gacggcccag cgtatgccgg
    21181 acggcatgat cctgctggag atggcaccga tggataacca gcgccgctta agtcaggaac
    21241 agctacagca cgcccagcag gttgctgccc gtgatttagt gcgcggcctg gcacatgaga
    21301 ttaaaaatcc gcttggcggt ttacgtggcg cggcgcagct gctcagcaaa gcattacctg
    21361 acccgtcact actcgaatat accaaagtga ttatcgaaca ggcggaccgg ctgcgaaatc
    21421 tggtcgaccg tctgttggga ccgcaactgc ccggtacgcg cgttaccgaa agtattcata
    21481 aagtagctga acgcgtggtg acgctggtgt cgatggaact gccggacaac gtgcggttga
    21541 ttcgggatta cgaccccagc ctgccggaac tggcgcacga cccggatcaa attgaacagg
    21601 tcttgctgaa tattgtgcgc aatgcgctac aggcgctggg gccggagggt ggtgaaatca
    21661 ttctgcgtac ccgcacagcg tttcaactga ccttacacgg cgagcgctat cggctggcgg
    21721 cgcgaattga tgtggaagat aacgggccag gtattccgcc tcatttgcag gatacgctgt
    21781 tttacccgat ggtcagcggc cgcgaaggtg gcaccgggct tggcttatcc atcgcccgta
    21841 atttgattga tcagcattca ggcaaaattg aatttaccag ttggccaggt cataccgagt
    21901 tctcggttta cctgcctatc aggaaataaa ggtgacgttt atgcaacgag ggatagtctg
    21961 ggtagtcgat gacgatagtt ccatccgttg ggtgcttgaa cgtgcgctcg ctggagcggg
    22021 tttaacctgt acaacatttg agaacggcgc ggaggtactg gaggcgctgg cgagcaaaac
    22081 gccggatgtg ctgctttcag atatccgtat gccgggaatg gacgggctgg cgctgctcaa
    22141 gcagattaaa cagcgccatc cgatgcttcc ggtcatcatt atgaccgcac attccgatct
    22201 ggatgctgcc gtcagcgcct atcaacaagg ggcatttgat tatctgccca aaccgtttga
    22261 tatcgacgaa gccgtggcgc tggttgagcg cgccatcagt cattaccagg aacagcagca
    22321 gccgcgtaat gttcagctta acggcccaac gaccgatatc atcggtgaag cgccagccat
    22381 gcaggacgtg ttccggatta tcggtcggct ttcgcgttct tctattagcg tgctgattaa
    22441 cggcgagtcc ggcaccggta aagaactggt cgctcatgcc ctgcatcgcc acagtccgcg
    22501 agccaaagcg ccgtttatcg cgctcaatat ggcggctatc ccgaaggatt tgatcgaatc
    22561 cgaactgttt ggtcacgaga aaggcgcatt taccggcgcg aataccattc gtcaggggcg
    22621 ttttgaacag gctgatggtg gcacattatt cctcgatgaa attggcgata tgccgctgga
    22681 tgtgcagacg cgtttgctgc gcgtactggc agatggtcag ttttatcgcg ttggcggcta
    22741 tgcgccggtg aaagtagatg tgcggattat cgctgccact caccagaatc ttgaacagcg
    22801 ggtgcaggaa ggtaagttcc gtgaggatct gttccaccgc ctgaacgtca tccgcgttca
    22861 tctgccgcca ctgcgtgagc gtcgggaaga tattccccgt ctggcacgcc attttttaca
    22921 ggttgccgcg cgcgaactgg gcgtagaagc gaagttgctg catccggaaa ccgaagccgc
    22981 gctgacgcgc ctggcgtggc cggggaacgt gcgccagctg gaaaacacct gtcgctggct
    23041 aacggtgatg gccgccgggc aggaagtgtt gattcaggat ttgcccggtg aactgtttga
    23101 atcaacggtt gcggagagta cttctcaaat gcaaccggac agttgggcga cacttttagc
    23161 gcagtgggca gacagagcgc tgcgttccgg tcatcaaaat ctgctttccg aagcgcagcc
    23221 tgagctggag cggacgttac tgacgaccgc gttgcgacat acgcaagggc ataaacagga
    23281 agcggcgcgg ctactcggct gggggcgtaa cactctgacg cggaagttaa aagagctggg
    23341 gatggagtga ttcacggctt gtgtgtaaag attgattatt gagcgcaaat tgctggtgtt
    23401 ttgcgcttta ctgttccgat aagttcagta tgatcttgcc cggaaaacgg ggagagtcat
    23461 tatgctggaa tcaataatta atctggtatc gagtggcgca gttgacagcc acacaccgca
    23521 aactgctgtt gctgccgtgc tgtgtgccgc gatgattggg ctgtttagtt gagtttcaga
    23581 ttgctgatga agtgctcttt gttcatgccg gatgcggcgt gaacgcctta tccggcctac
    23641 aaaagcaata ttttcaacag attttgcagg cctgataagc gtagcgcatc aggcaatctt
    23701 gttctcagca cattgatggc gctttcgttt tctactttgt aaacgaagcg ccattcacaa
    23761 tttaaatcac ccgagagaac tgctgcatcc gcgctttctg gcgcagatag gtatcaaagc
    23821 acatgcaaat gttgcggatc agcaagcgac ctttcgccgt cacctgtatt cccttctcat
    23881 ccacatccac cagcccatct tttgctaacg gggcgagcag cttgagatct tcggcaaagt
    23941 aatcagcgaa gagcaaatcc cactgttgct caatgggagc gtaatccaga cggaagttgc
    24001 agatgagcga cttaatcaca tcgcggcgaa tacagtcatc acgcgttagc gcaataccac
    24061 gccacagcgc attgccttgt tcatccactt gctgatagta ctgcttcaac tctttctggt
    24121 tctgcgcgta gcagtcgcca atcatgctga tggcggaaac gcccatcccc agcagatcgg
    24181 tatcgccctg agtggtgtag ccctggaagt tacgatgcag cacgccttca cgctgggcca
    24241 ccgccagctc gtcatccgga cgggcaaagt gatccatacc gataaactga tagcccgatt
    24301 gtgtcaggaa ggcgatggtt tcctgcagga tatcgagttt ttgctgcgga ctcggcaggt
    24361 cagcatcttt gattttgcgc tgagcagcaa aaatggtcgg cagatgcgcg tagttaaaga
    24421 cactcagacg atcggggttc agctccgcca cacgtttcag ggtaaaggcg aaactctccg
    24481 gcgtctgttt cggcaggccg taaatcaggt cgatgttggt ggaggtaaag ccaatctcac
    24541 gcgcatggtt aagcagtgca aagatgaact cttcatcctg ctcgcggtta accagacgtt
    24601 gcacttcttt gttgaagtcc tgcacgccca tgctcaggcg attaaagcct tcggcgcgta
    24661 aatgatcgag tacatccagt tcgatttccc gcggatcgac ttcgatcgag atctccgcat
    24721 cggcattgaa ctggaagttt tcgcgcagca gcttcatcag acggctgatt tgcgctttat
    24781 tcagatacgt cggcgttccg ccgccccagt gcaattggct gacgtgacgc ccggcgaaca
    24841 gcggtgcacg atggacgatt tcttgctcca gcgcgtccag atactgatcg gccttgtgct
    24901 gctggcgagt aactatctta ttgcaaccgc agaagtaaca aagcttatgg cagaacggaa
    24961 tatgtacgta gagagataat ggacgctcag gatagcgcgc cacggcttgt aaaaacgcct
    25021 gttcgccgaa gtcttctgaa aactccagcg cggtcgggta cgaggtgtat cgtggcccgg
    25081 aatagttata tttctggatc agggccagat cccagtcgat ttgctgtaca gacatgctca
    25141 ctccttccga tggcgtctct ggcggctacg gcgtaccggc ggatgcccgt ttcgagtcat
    25201 cagccggttg cgcagccact tttgtcgccg cgacaaccgc tgtagtttaa cgaataacca
    25261 caccagataa catataaccg gaagggttat cagcagaacg ggaaggccca cggcggcaat
    25321 ccgttagttg ccccgcagca gacgcatcat gtcttcttgc ttctcgtctt cttcctcttc
    25381 ttcgtcatcg tcataagaga ggccgagttt ctgcatcaac tcatcaatac gatccagttt
    25441 ggcatccacc caggattgct cttcggcgct cagggtttcg cctgcttcca gacgttccag
    25501 cagcgcatcc agacgctcat ccgtttccag taactccaac tccgcctgcg gtgaaagcat
    25561 aggtttctca ctcttcggtt tgtgctgttt ggtgactttt tcagtcacgc ccaatggaat
    25621 aggggtttta ctgccaatac gtggatcttt cggtgcgttc tggcctttgc tgcctgacgt
    25681 ggtgttaccg cccgctgcgc ggctgcccgg cgcatgacca cgacgttttt tctgacgctt
    25741 acggtcacga gcttcctgat ccagctcctc gcgtgttttt cgacgcgctt ttgcatgacc
    25801 tttgctgcgt gagtttgaag atgatggttt cataatggtt cgtcttttag ccgttttatt
    25861 cagtatagat ttgcggcgga atctaacaga aagcaagcaa agaaaaaagg cgacagatta
    25921 ctctgtcgcc ttttttcctg actcataacc cttaacgggt cttacaatcc ttgcaggccg
    25981 ccaacacacc ctgtttttcc attagccgga acaatccgtg ttgattccat ttccgtttaa
    26041 ttacgtctcc ggacgtttat cttcctgaca atcctctgtc ttcgcctcct ggcgctcctg
    26101 accctcatcc tgagtcattg ttcctgttag cgtcctcgct ctatgggtgc tactttacgc
    26161 ctttgcttta aataaacaat caaccgccat cagtatttgc acattttcag agttttatat
    26221 aaaagcaatt cactgattta tatagcttat ttgacatacg tcgcagaatt ttctctgcta
    26281 tttcgcataa agactatcgg caatgtctca caaagcatat ggcttttact tacaacgctc
    26341 acgccgaaaa atccgccttt tattatggtt caggtataat cgccgcaaag ctttgactga
    26401 tggagacgac cgctttgact aatttgaatt atcaacagac gcattttgtg atgagtgcgc
    26461 ctgatattcg ccacctacct tccgataccg gaattgaagt ggcttttgca ggccgttcca
    26521 acgcaggtaa atccagcgcg ctgaacacgc tgactaacca gaaaagcctg gctcgtacct
    26581 caaaaacccc ggggcgcacc cagcttatca acctgtttga agtggctgac ggcaagcgtc
    26641 tggttgactt gcctgggtac ggttatgcgg aagtcccgga agagatgaag cgcaaatggc
    26701 agcgcgcgct cggcgagtac ctcgaaaaac gtcagagcct gcaaggtctg gtggtgctaa
    26761 tggatattcg ccatccgctg aaagatttgg atcagcagat gattgagtgg gcggtagaca
    26821 gcaatatcgc cgtgctggta ctgctgacca aagcggacaa actggcaagc ggcgcacgta
    26881 aagcgcaatt gaatatggtg cgtgaagcgg tgctggcgtt taacggtgat gtgcaggttg
    26941 aaacgttttc ttcgctgaag aaacaaggcg tggacaagct gcggcagaaa ctggatacct
    27001 ggtttagcga gatgcagcct gtagaagaaa cgcaggacgg cgaataattt tcttgcctta
    27061 atgcttgtgc cggatgtggc gtatcaggca ttacggatct tttctttcgc ccaataaaaa
    27121 acgccccagt cattactgac tggggcggct aaaatattca gccaaatccg attacgtgaa
    27181 gtaaaaggtc tgaaagatag aacatcttac ctctgtaccc tacgcgacta actttactct
    27241 tttttgttca gttcagaaag cattttttgt aatttttttt cagttgttgc ataggaattt
    27301 ctaacagcca tcacaaaacg tgacagctta tgttgcttac ttacgaaaaa agggcatgtt
    27361 caggcgaatc ttagtgcgcc tgatcccagt tttcgccact ccccacttcc accagcagcg
    27421 gcacatccag acgggtacag ttttccatca gttgatgaat ctgcttcgcg acggcatcga
    27481 catcatcttt atgaacttca aataccagtt catcgtgtac ctgcatgatc atacgcacac
    27541 gcggttgctc agcctgtagc cacgcatcaa cggcaatcat cgcccgtttg ataatgtcgg
    27601 cggcggttcc ctgcattggc gcgttaatgg ctgcacgttc agccgctgca cgacgagccc
    27661 cattgctgga tttgatatcc ggcagataca gacggcgtcc gtccagcgtt tcaacgtagc
    27721 cctgctcttt cgcctgagca cgggtgcgtt ccatatactc cagcacgcca gggtagcgtt
    27781 cgaagtaaag gtccatgtac ttctgcgctt ctttacgtgg aatgttcaat tgccgcgcca
    27841 gaccgaaagc actcatgcca taaatcagac caaagttgat cgctttcgcg ctacggcgtt
    27901 gctcgctggt tacggtttcc agtggcaaac caaacacttc tgccgccgta gcccggtgga
    27961 tatcttttcc ttccgcgaat gcggtcagca agcctttgtc acgcgagaga tgcgccataa
    28021 tgcgcagttc aatctgcgag tagtccgccg agacaatcac ataatcctct ggcgcaataa
    28081 acgcctggcg gatacgacga ccttcttcgt tacgcaccgg aatgttttgc aggttaggat
    28141 cggttgacga taaacgtccc gttgcagtta ctgcctggtg ataagaggta tgcacacgcc
    28201 cggttttcgg gttgatcatc agcggcagct tgtcggtgta agtcgatttc agcttcgcca
    28261 gaccacgata ctccagaatc acttttggca acggatagtc cagcgccagt tcttccagta
    28321 cctcttccga cgttgacggc gcgccacccg gcgttttctt cagcggttta attccctgtt
    28381 tttcaaacag aatggtttgc aactgcttgg tggaggaaag gttaaattct tcacctgcaa
    28441 tttcatgcgc tttcttttcc agctcagcca gacgaagagt gagctcttca gaatgattgt
    28501 gcagcacttt cggatcgatc ttcacaccgt tacgttcaat gcgtgaaagc accggcacta
    28561 gcggcatttc gatattctcg aagacgttca acggcccttt gtgtttttgc agatccggcc
    28621 acattttcag atgcaactgc aaggtgacat ctgcatcttc ggcggcgtaa cgtccggcct
    28681 cttcgagggc aatctggtta aaggtcagtt gatttttgcc tttacccgca atctcttcaa
    28741 aagtgatggt tttgtgcttc aaccaacgtt ccgcgaggct gtccatatcg tgacgcccgg
    28801 caacgctatt gagaatgtag gactccagca tggtatcaaa cgcaatccca cgcagctcaa
    28861 tgccgtaatt cgccagaata ccgcgatcgt atttcaggtt ttgcccaacc ttcagcgcct
    28921 tttcatcttc cagcagcggt tttagcaact cgagtgcacg ctcgcgagag atttgatcgg
    28981 gcgcatcaag ataatcatga gcaaccggaa tatatgccgc tacgcctggc tcgatagcaa
    29041 aagaaagccc gaccaggtta gcagagatgt tatcaaggct gtcggtttcg gtatcaaatg
    29101 caaataccgg cgctttttcc agcttcgcaa tccacgcttt cagtgtttct tcatcaagga
    29161 tggtgacgta gttgtcataa gaaatcaccg ttgccgtcac ttctggcgct tcgtctacaa
    29221 cactggtttc ctgcggcttc gcggctggtt ttgccccttt ggcctgtaac catttgcccg
    29281 cttcgacatc agcagtccag cgtttgaact catacttttt gaacagccct aacaactctt
    29341 ctgccaccgg tggctgcact tccagttgtt cacaggtcag ctccagttca acgtcggttt
    29401 taatcgtcgc cagctggtaa gagagataag ccacttcttt gttttgctcg agcttcgccg
    29461 ccattgtttt cgcgccacgg aagctcaacc cagcaatttt ttctggctcg gcatacagcg
    29521 tatccagtcc gccaagacct tgtagcaatg cttgcgcggt tttttcaccg acgcccggta
    29581 cgccagggat gttatcagag gagtcaccca tcagcgccag gaagtcgatg atcagttctg
    29641 gcggcacgcc gtacttattc accacctctt ccgggccgag gatggtattc gtcatggtat
    29701 tgataagcgt aatatttggc gtcaccagct gcgccatatc tttatcgcct gtgcttatca
    29761 gcaccggacg cccggctttt tcggcttccc gcgccagagt accgataacg tcgtccgctt
    29821 ctacgccaga aaccgccagc agcggcagtc ccatcgcttt aaccatcgcg tgcaagggtt
    29881 cgatttgtgc acgcagatcg tccggcattg gcgggcgatg tgatttgtaa tgttcaaaca
    29941 gttcatcacg aaaggttttt cccttggcgt caaagaccac cgctgcatgc gtcggtttat
    30001 attgcatgat tagactgcgc agcatgttga ggacaccata catcgcaccg gtcggctcgc
    30061 ctgcgctgtt agtcagcggg ggaaacgcgt gatatgcgcg ataaagataa gatgaaccat
    30121 ctacaaggat aagtggattt tgggggatct gaaccataat gtccgtgcct gtttatcaga
    30181 ttatgggtaa aggatgccac agacaggatg aaaacatgag tttttcaccg cttttcgtga
    30241 aaatcttttg tagatcttct ggatcgctcg caaatcgtca tgtggataac tttgtgcata
    30301 aattatttac at
//
LOCUS       DAZFLD010000031        30292 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.031, whole genome shotgun sequence.
ACCESSION   DAZFLD010000031 DAZFLD010000000
VERSION     DAZFLD010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30292
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.031"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            65..1648
                     /gene="yegH"
                     /locus_tag="ACHKBL_001223"
     CDS             65..1648
                     /gene="yegH"
                     /locus_tag="ACHKBL_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HFO5818117.1"
                     /translation="MEWIADPSIWAGLITLIVIELVLGIDNLVFIAILAEKLPPKQRD
                     RARVTGLLLAMLMRLLLLASISWLVTLTQPLFSFRSFTFSARDLIMLFGGFFLLFKAT
                     MELNERLEGKDSNNPTQRKGAKFWGVVTQIVVLDAIFSLDSVITAVGMVDHLLVMMAA
                     VVIAISLMLMASKPLTQFVNSHPTIVILCLSFLLMIGFSLVAEGFGFVIPKGYLYAAI
                     GFSVMIEALNQLAIFNRRRFLSANQTLRQRTTEAVMRLLSGQKEDAELDAETASMLVD
                     HGNQQIFNPQERRMIERVLNLNQRTVSSIMTSRHDIEHIDLNAPEEEIRQLLERNQHT
                     RLVVTDGDDAEDLLGVVHVIDLLQQSLRGEPLNLRVLIRQPLVFPETLPLLPALEQFR
                     NARTHFAFVVDEFGSVEGIVTLSDVTETIAGNLPNEVEEIDARHDIQKNADGSWTANG
                     HMPLEDLVQYVPLPLDEKREYHTIAGLLMEYLQRIPKPGEEVQVGDYLLKTLQVESHR
                     VQKVQIIPLHKDGEMEYEV"
     gene            1723..2061
                     /locus_tag="ACHKBL_001224"
     CDS             1723..2061
                     /locus_tag="ACHKBL_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO5818118.1"
                     /translation="MRKKLAFLDTSLDDLRAFPESSRQEIGYQLDRIQQGLNPYDWKP
                     FSTIGPGVREIRTRDADGIYRVMYVAKFEEAVYVLHCFQKKTQTTSQSNIDLAKRRYK
                     ELVQERKNEN"
     gene            2051..2341
                     /locus_tag="ACHKBL_001225"
     CDS             2051..2341
                     /locus_tag="ACHKBL_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO5818119.1"
                     /translation="MKIETFDSVWDAVSDTPEQAENMRIRAELVTIINNWIEQQGFSQ
                     AQAASALGVTQPRISELARGKIQIFSIDKLITMMAHAGLHIQRIEVQYPHAA"
     gene            complement(2394..4247)
                     /gene="asmA"
                     /locus_tag="ACHKBL_001226"
     CDS             complement(2394..4247)
                     /gene="asmA"
                     /locus_tag="ACHKBL_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane assembly protein AsmA"
                     /protein_id="HFO5818120.1"
                     /translation="MRRFLTTLMILLVVLVAGLSALVLLVNPNDFRDYMVKQVAARSG
                     YQLQLDGPLRWHVWPQLSILSGRMSLTAQGASQPLVRADNMRLDVALLPLLSHQLSVK
                     QVMLKGAVIQLTPQTEAVRSEDAPVAPRDNTLPDLSDDRGWSFDISSLKVADSVLVFQ
                     HEDDEQVTIRNIRLQMEQDPQHRGSFEFSGRVNRDQRDLTISLNGTVDASDYPHDLTA
                     AIEQVNWQLQGADLPKQGIQGQGSFQAQWQESHKRLSFNQISLTANDSTLSGQAQVTL
                     TEKPEWQLRLQFPQLNLDNLIPLKETANGENGAAQQGQSQSTLPRPVISSRIDEPAYQ
                     GLQGFTADILLQASNVRWRGMNFTDVATQMTNKSGLLEITQLQGKLNGGQVSLPGTLD
                     ATSINPRINFQPRLENVEIGTILKAFNYPISLTGKMSLAGDFSGADIDADAFRHNWQG
                     QAHVEMTDTRMEGMNFQQMIQQAVERNGGDVKAAENFDNVTRLDRFTTDLTLKDGVVT
                     LNDMQGQSPVLALTGEGMLNLADQTCDTQFDIRVIGGWNGESKLIDFLKETPVPLRVY
                     GNWQQLNYSLQVDQLLRKHLQDEAKRRLNDWAERNKDSRNGKDVKKLLEKM"
     gene            complement(4269..4850)
                     /gene="dcd"
                     /locus_tag="ACHKBL_001227"
     CDS             complement(4269..4850)
                     /gene="dcd"
                     /locus_tag="ACHKBL_001227"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004942378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006229 - dUTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HFO5818121.1"
                     /translation="MRLCDRDIEAWLDEGRLSINPRPPVERINGATVDVRLGNKFRTF
                     RGHTAAFIDLSGPKDEVSAALDRVMSDEIVLDESEAFYLHPGELALAVTLESVTLPAD
                     LVGWLDGRSSLARLGLMVHVTAHRIDPGWSGCIVLEFYNSGKLPLALRPGMLIGALSF
                     EPLSGPAARPYNRREDAKYRNQQGAVASRIDKD"
     gene            complement(4942..5583)
                     /gene="udk"
                     /locus_tag="ACHKBL_001228"
     CDS             complement(4942..5583)
                     /gene="udk"
                     /locus_tag="ACHKBL_001228"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HFO5818122.1"
                     /translation="MTDQSHQCVIIGIAGASASGKSLIASTLYRELREQVGDEHIGVI
                     PEDCYYKDQSHLSMEERVKTNYDHPSAMDHSLLLEHLQALKRGSAIDLPVYSYVEHTR
                     MKETVTVEPKKVIILEGILLLTDARLRDELNFSIFVDTPLDICLMRRIKRDVNERGRS
                     MDSVMAQYQKTVRPMFLQFIEPSKQYADIIVPRGGKNRIAIDILKAKISQFFE"
     gene            5901..9218
                     /gene="dgcE"
                     /locus_tag="ACHKBL_001229"
     CDS             5901..9218
                     /gene="dgcE"
                     /locus_tag="ACHKBL_001229"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFO5818123.1"
                     /translation="MSKQSQHVLIALPHPLLHLVSLGLVSFIFTLFSLELSQFGTQLA
                     PLWFPTSIMMVAFYRHAGRMWPGIALSCSLGNIAASILLFSTSSLNMTWTTINIVEAV
                     VGAVLLRKLLPWYNPLQNLADWLRLALGSAIVPPLLGGVLVILLTPGDDPLRAFLIWV
                     LSESIGALALVPLGLLFKPHYLLRHRNPRLLFESLLTLAITLTLSWLSMLYLPWPFTF
                     IIVLLMWSAVRLPRMEAFLIFLTTVMMVSLMMAADPSLLATPRTYLMSHMPWLPFLLI
                     LLPANIMTMVMYAFRAERKHISESETRFRNAMEYSAIGMALVGTEGQWLQSNKALCQF
                     LGYSQEELRGLTFQQLTWPEDLNKDLQQVEKLISGEINTYSMEKRYYNRNGDVVWALL
                     AVSLVRHTDGTPLYFIAQIEDINELKRTEQVNQQLMERITLANEAGGIGIWEWELKPN
                     IFSWDKRMFELYEIPPHIKPNWQVWYECVLPEDRQHAEKVIRDSLQSRSPFKLEFRIA
                     VKDGIRHIRALANRVLNKEGEVERLLGINMDMTEVKQLNEALFQEKERLHITLDSIGE
                     AVVCIDMAMKITFMNPVAEKMSGWTQEEALGVPLLTVLHITFGDNGPLMENIYSADTS
                     RSAIEQDVVLHCRSGGSYDVHYSITPLSTLDGSNIGSVLVIQDVTESRKMLRQLSYSA
                     SHDALTHLANRASFEKQLRILLQTVNSTHQRHALVFIDLDRFKAVNDSAGHAAGDALL
                     RELASLMLSMLRSSDVLARLGGDEFGLLLPDCNVESARFIATRIISAVNDYHFIWEGR
                     VHRVGASAGITLIDDNNHQAAEVMSQADIACYASKNGGRGRVTVYEPQQAAAHSERAV
                     MSLDEQWRMIKENQLMMIAHGVASPRIPEARNLWLISLKLWSCEGEIIDEQTFRRSFS
                     DPALSHALDRRVFHDFFQQAAKAIASKGLSIALPLSVAGLSSATLVNELLEQLENSPL
                     PPRLLHLIIPADAILDHAESVQKLRLAGCRIVFSQVGRDLQIFNSLKANMADYLLLDG
                     ELCANVQGNLMDEMLITIIQGHAQRLGMKTIAGPVVLPLVMDTLSGIGVDLIYGDVIA
                     DAQPLDLLVNSSYFAIN"
     gene            complement(9327..10175)
                     /gene="alkA"
                     /locus_tag="ACHKBL_001230"
     CDS             complement(9327..10175)
                     /gene="alkA"
                     /locus_tag="ACHKBL_001230"
                     /EC_number="3.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2"
                     /protein_id="HFO5818124.1"
                     /translation="MYTLNWQPPYDWSWMLGFLAARAVNGVETVADDYYARSLAVGEC
                     RGVVTAIPDIASHTLHINLSAGLEPVAAECLAKMSRLFDLQCNPQIVNGALGKLGAAR
                     PGLRLPGCVDAFEQGVRAILGQLVSVAMAAKLTARVAQLYGERLDDFPDYVCFPTPQR
                     LAAADPQALKALGMPLKRAEALIHLANAALEGTLPMTIPGDVEQAMKTLQTFPGIGRW
                     TANYFALRGWQAKDVFLPDDYLIKQRFPGMTPAQIRRYAERWKPLRSYALLHIWYTEG
                     WQPDEA"
     gene            10309..11661
                     /gene="yegD"
                     /locus_tag="ACHKBL_001231"
     CDS             10309..11661
                     /gene="yegD"
                     /locus_tag="ACHKBL_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFO5818125.1"
                     /translation="MFIGFDYGTANCSVAVMRDGKPQLLKMENDSTLLPSMLCAPTRE
                     AVSEWLYRHHDVPADDDETQALLRRAIRYNREEDIDVTAKSVQFGLSSLAQYIDDPEE
                     VWFVKSPKSFLGASGLKPQQVALFEDLVCAMMLHIRQQAQAQLPEAITQAVIGRPINF
                     QGLGGDEANAQAQGILERAAKRAGFKDVVFQYEPVAAGLDYEATLQEEKRVLVVDIGG
                     GTTDCSLLLMGPQWRSRLDREASLLGHSGCRIGGNDLDIALAFKNLMPLLGMGGETEK
                     GIALPILPWWNAVAINDVPAQSDFYSSANGRLLNDLVRDAREPEKVALLQKVWRQRLS
                     YRLVRSAEESKIALSSVAETRASLPFISDELATLISQQGLESALSQPLARILEQVQLA
                     LDNAQEKPDVIYLTGGSARSPLIKKALTEQLPGIPIAGGDDFGSVTAGLARWAEVVFR
                     "
     gene            complement(11674..13613)
                     /gene="yegI"
                     /locus_tag="ACHKBL_001232"
                     /pseudo
     CDS             complement(11674..13613)
                     /gene="yegI"
                     /locus_tag="ACHKBL_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310906.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YegI"
     gene            complement(13610..14371)
                     /gene="pphC"
                     /locus_tag="ACHKBL_001233"
     CDS             complement(13610..14371)
                     /gene="pphC"
                     /locus_tag="ACHKBL_001233"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase PphC"
                     /protein_id="HFO5818126.1"
                     /translation="MSWRLVYASTVGTSHISADLPCQDACQMQVAWLNDQQPLLSVFV
                     ADGAGSASQGGEGAMLAVNEAMAYMSQKVQGGELGLNDVLATDIVLTIRQRLFAEAEA
                     KELAVRDFACTFLGLISSANGTLIMQIGDGGVVVDLGHGLQLPLTPMVGEYTNMTHFI
                     TDEDAVSRLETFTSTERAHKVAAFTDGIQRLALNMLDNSPHVPFFTPFFNGLASATQE
                     QLDLLPELLKQFLSGPAVNERTDDDKTLALALWLP"
     gene            complement(14368..15027)
                     /gene="yegL"
                     /locus_tag="ACHKBL_001234"
     CDS             complement(14368..15027)
                     /gene="yegL"
                     /locus_tag="ACHKBL_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HFO5818127.1"
                     /translation="MSEQITFATSDFASNPEPRCPCILLLDVSGSMNGRPINELNAGL
                     VTFRDELLADPLALKRVELGIVTFGPVHVEQPFTSAANFFPPILFAQGDTPMGAAITK
                     ALDMVEERKREYRANGISYYRPWIFLITDGAPTDEWQAAANKVFQGEEDKKFAFFSIG
                     VQGADMKTLAQISVRQPLPLQGLQFRELFSWLSSSLRSVSRSTPGTEVVLEAPKGWTS
                     V"
     gene            complement(15248..15307)
                     /gene="ibsA"
                     /locus_tag="ACHKBL_001235"
     CDS             complement(15248..15307)
                     /gene="ibsA"
                     /locus_tag="ACHKBL_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsA"
                     /protein_id="HFO5818128.1"
                     /translation="MMKHVIILVILLVISFQAY"
     gene            complement(15580..15636)
                     /locus_tag="ACHKBL_001236"
     CDS             complement(15580..15636)
                     /locus_tag="ACHKBL_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFO5818129.1"
                     /translation="MMKLLIIVVLLVISWPAY"
     gene            complement(15908..15964)
                     /locus_tag="ACHKBL_001237"
     CDS             complement(15908..15964)
                     /locus_tag="ACHKBL_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFO5818130.1"
                     /translation="MMKWIIIVVLLVISFPAY"
     gene            complement(16236..16292)
                     /locus_tag="ACHKBL_001238"
     CDS             complement(16236..16292)
                     /locus_tag="ACHKBL_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFO5818131.1"
                     /translation="MMKILIIVVLLVVSYPAY"
     gene            16571..17818
                     /gene="mdtA"
                     /locus_tag="ACHKBL_001239"
     CDS             16571..17818
                     /gene="mdtA"
                     /locus_tag="ACHKBL_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter subunit MdtA"
                     /protein_id="HFO5818132.1"
                     /translation="MKSSYKSRWVMVIVVVIAAIAAFWFWQGRNDSRSAAPGATKQAQ
                     QSPAGGRRGMRSGPLAPVQAATAVEQAVPRYLTGLGTITAANTVTVRSRVDGQLMALH
                     FQEGQQVKAGDLLAEIDPSQFKVALAQAQGQLAKDKATLANARRDLARYQQLVKTNLV
                     SRQELDAQQALVSETEGTIKADEASVASAQLQLDWSRITAPVDGRVGLKQVDVGNQIS
                     SGDTTGIVVITQTHPIDLLFTLPESDIATVVQAQKAGKPLVVEAWDRTNSKKLSEGTL
                     LSLDNQIDATTGTIKVKARFNNQDDALFPNQFVNARMLVDTEQNAVVIPTAALQMGNE
                     GHFVWVLNSENKVSKHLVTPGIQDSQKVVIRAGISAGDRVVTDGIDRLTEGAKVEVVE
                     AQSATTPEEKATSREYAKKGARS"
     gene            17818..20940
                     /gene="mdtB"
                     /locus_tag="ACHKBL_001240"
     CDS             17818..20940
                     /gene="mdtB"
                     /locus_tag="ACHKBL_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtB"
                     /protein_id="HFO5818133.1"
                     /translation="MQVLPPSSTGGPSRLFIMRPVATTLLMVAILLAGIIGYRALPVS
                     ALPEVDYPTIQVVTLYPGASPDVMTSAVTAPLERQFGQMSGLKQMSSQSSGGASVITL
                     QFQLTLPLDVAEQEVQAAINAATNLLPSDLPNPPVYSKVNPADPPIMTLAVTSTAMPM
                     TQVEDMVETRVAQKISQISGVGLVTLSGGQRPAVRVKLNAQAIAALGLTSETVRTAIT
                     GANVNSAKGSLDGPSRAVTLSANDQMQSAEEYRQLIIAYQNGAPIRLGDVATVEQGAE
                     NSWLGAWANKEQAIVMNVQRQPGANIISTADSIRQMLPQLTESLPKSVKVTVLSDRTT
                     NIRASVDDTQFELMMAIALVVMIIYLFLRNIPATIIPGVAVPLSLIGTFAVMVFLDFS
                     INNLTLMALTIATGFVVDDAIVVIENISRYIEKGEKPLAAALKGAGEIGFTIISLTFS
                     LIAVLIPLLFMGDIVGRLFREFAITLAVAILISAVVSLTLTPMMCARMLSQESLRKQN
                     RFSRASEKMFDRIIAAYGQGLAKVLNHPWLTLSVALSTLLLSVLLWVFIPKGFFPVQD
                     NGIIQGTLQAPQSSSFTNMAQRQRQVADVILQDPAVQSLTSFVGVDGTNPSLNSARLQ
                     INLKPLDERDDRVQKVIARLQTAVDKVPGVDLFLQPTQDLTIDTQVSRTQYQFTLQAT
                     SLDALSTWVPQLMEKLQQLPQLSDVSSDWQDKGLVAYVNVDRDSASRLGISMADVDNA
                     LYNAFGQRLISTIYTQANQYRVVLEHNTENTPGLAALDTIRLTSSDGGVVPLSSIAKI
                     EQRFAPLSINHLDQFPVTTISFNVPDNYSLGDAVQAIMDTEKTLNLPVDITTQFQGST
                     LAFQSALGSTVWLIVAAVVAMYIVLGILYESFIHPITILSTLPTAGVGALLALLIAGS
                     ELDVIAIIGIILLIGIVKKNAIMMIDFALAAEREQGMSPRDAIYQACLLRFRPILMTT
                     LAALLGALPLMLSTGVGAELRRPLGIGMVGGLIVSQVLTLFTTPVIYLLFDRLALWTK
                     SRFARHEEEA"
     gene            20941..24018
                     /gene="mdtC"
                     /locus_tag="ACHKBL_001241"
     CDS             20941..24018
                     /gene="mdtC"
                     /locus_tag="ACHKBL_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtC"
                     /protein_id="HFO5818134.1"
                     /translation="MKFFALFIYRPVATILLSVAITLCGILGFRMLPVAPLPQVDFPV
                     IMVSASLPGASPETMASSVATPLERSLGRIAGVSEMTSSSSLGSTRIILQFDFDRDIN
                     GAARDVQAAINAAQSLLPSGMPSRPTYRKANPSDAPIMILTLTSDTYSQGELYDFAST
                     QLAPTISQIDGVGDVDVGGSSLPAVRIGLNPQALFNQGVSLDDVRSAISNANVRKPQG
                     ALEDDTHRWQIQTNDELKTAAEYQPLIIHYNNGGALRLGDVATVTDSVQDVRNAGMTN
                     AKPAILLMIRKLPEANIIQTVDSIRAKLPELQETIPAAIDLQIAQDRSPTIRASLEEV
                     EQTLIISVALVILVVFLFLRSGRATIIPAVAVPVSLIGTFAAMYLCGFSLNNLSLMAL
                     TIATGFVVDDAIVVLENIARHLEAGMKPLQAALQGTREVGFTVLSMSLSLVAVFLPLL
                     LMGGLPGRLLREFAVTLSVAIGISLLVSLTLTPMMCGWMLKASKPREQKRLRGFGRML
                     VALQQGYGKSLKWVLNHTRLVGVVLLGTIALNIWLYISIPKTFFPEQDTGVLMGGIQA
                     DQSISFQAMRGKLQDFMKIIRDDPAVDNVTGFTGGSRVNSGMMFITLKPRGERSETAQ
                     QIIDRLRVKLAKEPGANLFLMAVQDIRVGGRQSNASYQYTLLSDDLAALREWEPKIRK
                     KLATLPELADVNSDQQDNGAEMNLVYDRDTMARLGIDVQAANSLLNNAFGQRQISTIY
                     QPMNQYKVVMEVDPRYTQDISALEKMFVINNEGKAIPLSYFAKWQPANAPLSVNHQGL
                     SAASTISFNLPTGKSLSDASAAIDRAMTQLGVPSTVRGSFAGTAQVFQETMNSQVILI
                     IAAIATVYIVLGILYESYVHPLTILSTLPSAGVGALLALELFNAPFSLIALIGIMLLI
                     GIVKKNAIMMVDFALEAQRHGNLTPQEAIFQACLLRFRPIMMTTLAALFGALPLVLSG
                     GDGSELRQPLGITIVGGLVMSQLLTLYTTPVVYLFFDRLRLRFSRKPKQAVTE"
     gene            24019..25434
                     /gene="mdtD"
                     /locus_tag="ACHKBL_001242"
     CDS             24019..25434
                     /gene="mdtD"
                     /locus_tag="ACHKBL_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO5818135.1"
                     /translation="MTDLPDSTRWQLWIVAFGFFMQSLDTTIVNTALPSMAQSLGESP
                     LHMHMVIVSYVLTVAVMLPASGWLADKVGVRNIFFTAIVLFTLGSLFCALSGTLNELL
                     LARALQGVGGAMMVPVGRLTVMKIVPREQYMAAMTFVTLPGQIGPLLGPALGGLLVEY
                     ASWHWIFLINIPVGIIGAITTLMLMPNYTMQTRCFDLSGFLLLAVGMAVLTLALDGSK
                     GTGLSPLAIAGLVAVGVVALVLYLLHARNNNCALFSLKLFRTRTFSLGLAGSFAGRIG
                     SGMLPFMTPVFLQIGLGFSPFHAGLMMIPMVLGSMGMKRIVVQVVNRFGYRRVLVATT
                     LGLSLVTLLFMTTALLGWYYVLPFVLFLQGMVNSTRFSSMNTLTLKDLPDNLASSGNS
                     LLSMIMQLSMSIGVTIAGLLLGLFGSQHVSVDSGTTQTVFMYTWLSMAFIIALPAFVF
                     ARVPSDTHQNVAISRRKRSAQ"
     gene            25431..26834
                     /gene="baeS"
                     /locus_tag="ACHKBL_001243"
     CDS             25431..26834
                     /gene="baeS"
                     /locus_tag="ACHKBL_001243"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     BaeS"
                     /protein_id="HFO5818136.1"
                     /translation="MKFWRPGITGKLFLAIFATCIVLLITMHWAVRISFERGFIDYIK
                     HGNEQRLQMLGDALGEQYAQHGNWRFLRNNDRFVFQILRSLEHDNNEDKPGPGMPPHG
                     WRTQFWVVDQNNKVLVGPRAPVPPDGTRRPIMVNGAEVGAVIASPVERLTRNTDINFD
                     RQQRQTSWLIVALSTLLAALATFPLARGLLAPVKRLVDGTHKLAAGDFTTRVAPTSED
                     ELGRLAEDFNQLASTLEKNQQMRRDFMADISHELRTPLAVLRGELEAIQDGVRKFTPE
                     TLASLQAEVGTLTKLVDDLHQLSMSDEGALAYQKAPVDLIPLLEVAGGAFRERFASRG
                     LKLQFSLPDSITVFGDRDRLMQLFNNLLENSLRYTDSGGSLKISAEQHDKTVRLTFAD
                     SAPGVSDDQLQKLFERFYRTEGSRNRASGGSGLGLAICLNIVEAHNGRIIAAHSPFGG
                     VSITVELPLERDLQREV"
     gene            26831..27553
                     /gene="baeR"
                     /locus_tag="ACHKBL_001244"
     CDS             26831..27553
                     /gene="baeR"
                     /locus_tag="ACHKBL_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BaeR"
                     /protein_id="HFO5818137.1"
                     /translation="MTELPIDENTPRILIVEDEPKLGQLLIDYLRAASYAPTLISHGD
                     QVLPYVRQTPPDLILLDLMLPGTDGLTLCREIRRFSDIPIVMVTAKIEEIDRLLGLEI
                     GADDYICKPYSPREVVARVKTILRRCKPQRELQQQDAESPLMIDEGRFQASWRGKMLD
                     LTPAEFRLLKTLSHEPGKVFSREQLLNHLYDDYRVVTDRTIDSHIKNLRRKLEALDEE
                     QSFIRAVYGVGYRWEADACRIV"
     gene            27744..28076
                     /gene="yegP"
                     /locus_tag="ACHKBL_001245"
     CDS             27744..28076
                     /gene="yegP"
                     /locus_tag="ACHKBL_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HFO5818138.1"
                     /translation="MAGWFELSKSSDNQFRFVLKAGNGETILTSELYTSKASAEKGIA
                     SVRSNSPQEERYEKKTASNGKFYFNLKAANHQIIGSSQMYATAQSRETGIASVKANGT
                     SQTVKDNT"
     gene            28224..29585
                     /gene="yegQ"
                     /locus_tag="ACHKBL_001246"
     CDS             28224..29585
                     /gene="yegQ"
                     /locus_tag="ACHKBL_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HFO5818139.1"
                     /translation="MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFN
                     HENLQLGINEAHALGKKFYVVVNIAPHNAKLKTFIRDLKPVVEMGPDALIMSDPGLIM
                     LVREHFPEMPIHLSVQANAVNWATVKFWQQMGLTRVILSRELSLEEIEEIRNQVPDME
                     IEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYNVQEGKEDDVGNIVHKY
                     EPIPVQNVEPTLGIGAPTDKVFMIEEAQRPGEYMTTFEDEHGTYIMNSKDLRAIAHVE
                     RLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDTSLLETLEGLAHRGY
                     TEGFLRRHTHDDYQNYEYGYSVSDRQQFVGEFTGERKGELAAVAVKNKFSVGDSLELM
                     TPQGNINFTLEHMENAKGEAMPVAPGDGYTVWLPVPQDLELNYALLMRNFSGETTRNP
                     HGK"
     gene            complement(29927..30244)
                     /gene="yegR"
                     /locus_tag="ACHKBL_001247"
     CDS             complement(29927..30244)
                     /gene="yegR"
                     /locus_tag="ACHKBL_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YegR"
                     /protein_id="HFO5818140.1"
                     /translation="MKKIAAISLISVFLMSGCAVHNDETSIGKFGLAYKSNIQRKLDN
                     QYYTEAEASLARGRISGAENIVKNDAVHFCVTQGKKMQIVDLKTEGAGLHGVARLTFK
                     CGE"
BASE COUNT         6991 a         8083 c         7995 g         7223 t
ORIGIN      
        1 gccccgattt atccatgatc gaattgtgac atttgtcata caacgaatag gttttgtact
       61 tactatggaa tggattgccg atccgtctat ctgggccggg ttaatcacgc tgattgtgat
      121 cgaactggtc ctcggcattg ataacctggt ctttattgcc atcctcgccg aaaaactacc
      181 gccgaagcag cgcgaccgcg cacgggttac cgggctgctg ctggcgatgt taatgcgcct
      241 gttactgctg gcgtcaatct cctggctggt caccctgact caaccgctgt tcagcttccg
      301 ctcgtttacc tttagcgccc gcgacttaat catgctgttt ggtggtttct tcctgttgtt
      361 caaagccacg atggagctga acgaacggct ggaagggaaa gacagcaata accccaccca
      421 acgcaaaggt gcgaagttct ggggggtggt gacacaaatt gtggtactgg acgccatctt
      481 ctcactcgac tcggtgatta ccgccgtcgg gatggtcgac catttactgg tcatgatggc
      541 cgccgtggtt atcgctatca gcctgatgtt gatggccagc aagccgttaa cgcaatttgt
      601 taacagtcac ccgaccatcg ttattctctg cctaagtttc ctgttgatga ttggctttag
      661 cctggtggca gaaggtttcg gcttcgtcat tccgaaaggc tacctgtacg ctgccattgg
      721 tttctcggtg atgatcgagg cgctcaatca gttggctatc tttaaccggc gacgttttct
      781 ttccgctaac cagacactgc gccagcggac taccgaagcg gtaatgcgcc tgcttagcgg
      841 gcaaaaagaa gatgcggaac tggacgccga aaccgcgtct atgctggtgg atcatggtaa
      901 ccagcagatc tttaatccgc aggaacggcg gatgattgag cgggtactta atcttaacca
      961 gcgtaccgtc agcagcatta tgacgtcgcg ccacgatatt gagcatatcg atctcaacgc
     1021 gccggaagaa gaaatccgcc agttactgga gcgaaatcag catacgcggc ttgtcgttac
     1081 tgatggcgat gacgcagaag atttgctcgg tgttgttcac gttatcgacc tgttacaaca
     1141 gtctctgcgc ggcgaaccgc tcaacctgcg ggtgttgatt cgccagccgc tggtgttccc
     1201 ggaaaccttg ccgttgttac ctgccctgga gcagttccgt aatgcccgca cacactttgc
     1261 ttttgtggtg gatgagtttg gctcggtgga agggattgtg acattaagtg acgtcactga
     1321 aaccattgcc ggtaacttac cgaacgaagt ggaagagatc gacgcccgcc atgatattca
     1381 gaagaatgcg gacggttcct ggacggcgaa tggtcatatg ccgctggaag atctggtgca
     1441 atatgtgccg ctaccgctgg atgagaaacg cgaatatcac accattgccg ggctgttgat
     1501 ggaatatttg caacgtattc caaaacctgg cgaagaagtt caggtggggg attatttgct
     1561 taaaacgttg caggtggaaa gccatcgcgt gcagaaggtg cagattattc cgctgcataa
     1621 ggatggcgag atggagtacg aggtgtgata aaacatcaaa tgcgcaggtt caaactgcgc
     1681 atttgattta tacaaaattt agtataatct aaggatggag taatgagaaa aaagctggct
     1741 tttcttgata ccagtcttga cgatttacgc gcatttccag aatcgtctcg acaggaaatt
     1801 ggctatcaac tcgacaggat ccaacaaggg ttaaacccat acgactggaa acctttttca
     1861 actatcggcc ctggtgtgcg agagattcgt actcgcgatg ctgatgggat ctatcgcgtc
     1921 atgtatgtcg caaaatttga agaagcggtt tacgtattgc attgttttca aaagaaaaca
     1981 cagactacca gtcaatcgaa tattgattta gccaaacgac gctataaaga acttgttcag
     2041 gagcggaaaa atgaaaattg aaacctttga tagtgtgtgg gatgcggtga gtgacacccc
     2101 tgaacaagcg gaaaatatgc ggatccgtgc cgaacttgtt acgatcatta ataactggat
     2161 tgaacaacaa gggtttagcc aggctcaggc agcgtctgct ttaggggtca ctcaacctcg
     2221 catatctgaa cttgccagag gtaagatcca gatctttagt atcgataaat tgatcaccat
     2281 gatggctcat gcaggattgc atattcaacg tattgaagtt caatatccac atgctgctta
     2341 gtactataaa tacagatggc gacaaattga cgccgccatc tgcataactt gtcttacatc
     2401 ttctccagca acttcttcac atctttgcca ttgcgggaat ctttattccg ctcggcccag
     2461 tcattcaggc gacgtttcgc ttcgtcctgt agatgtttgc gcagtaactg atccacttgc
     2521 aggctgtaat taagttgctg ccagttgcca taaacccgca gcggtactgg cgtttctttg
     2581 aggaaatcaa tcagtttgct ttccccgttc caaccgccaa tcacacgaat gtcaaactgg
     2641 gtgtcgcagg tttgatctgc cagattcaac atgccttccc ctgtcagcgc cagcacaggc
     2701 gattgacctt gcatgtcgtt taacgtcacg acgccatcct tcaacgtcaa atcggtggta
     2761 aagcggtcaa gacgcgttac gttatcgaag ttttcagcgg ccttcacatc accaccatta
     2821 cgttctaccg cttgctgaat catctgctgg aagttcatcc cttccatgcg cgtgtcggtc
     2881 atttcgacat gtgcctgtcc ttgccagtta tggcggaatg cgtcggcatc tatgtcagca
     2941 ccggagaaat caccagccag tgacatcttt ccggtcaacg aaatcggata gttaaacgcc
     3001 ttcagaatgg tgccaatctc aacgttttcc agccgtggct ggaagtttat tcgcggattt
     3061 attgatgtcg cgtccagcgt gcccggcagt gaaacctgtc caccgttaag tttgccctgc
     3121 agttgagtaa tttccagcag acccgacttg ttggtcattt gcgtggcaac atctgtaaaa
     3181 ttcattccgc gccagcgcac gttactggcc tgcaacaaaa tatcagccgt aaagccttgc
     3241 agtccctgat aggccggttc atcaatacgc gaagaaatga ccgggcgcgg caacgtggat
     3301 tggctctgcc cctgctgcgc ggcaccgttt tcaccgttcg ctgtttcttt aagcgggatg
     3361 aggttgtcaa gattcagttg cgggaattgc agcctcagct gccattccgg tttctctgtc
     3421 agcgtgacct gtgcttgccc gctcagcgta ctgtcattgg cggtcaagct gatttggtta
     3481 aatgaaaggc gtttatgtga ctcctgccac tgggcctgga aactcccctg cccctgaata
     3541 ccttgttttg gtaaatctgc accctgcaac tgccagttaa cttgttcaat agccgccgtt
     3601 aaatcatgcg gataatcaga agcatctacc gtaccgttaa gggatatcgt gagatcgcgc
     3661 tgatcgcgat taacccgccc ggagaactca aatgaaccac gatgttgggg atcttgttcc
     3721 atttgcaggc gaatattgcg gattgtcacc tgctcgtcat cttcatgctg gaacaccagc
     3781 acgctgtccg ccaccttaag actggatata tcaaacgacc atccgcgatc gtctgacaga
     3841 tccggcaagg tattgtcgcg cggtgcaacc ggagcgtctt cactgcgcac cgcttccgtc
     3901 tgcggcgtca gttggatcac tgcccctttt agcatcacct gcttaacgct cagttgatga
     3961 ctcagcagtg gtaaaagcgc cacgtccaga cgcatgttgt ctgcgcgaac cagtggctgg
     4021 ctcgcgccct gggcagtgag agacattcgc ccggagagga tactaagctg cggccagacg
     4081 tgccaacgca gtggcccgtc gagctgcaat tgataaccgc tacgtgcagc aacttgcttg
     4141 accatatagt cgcggaaatc attcggattc accagcaaca ctaacgcaga taacccggcc
     4201 accagcacga ccaggagtat catcagcgtc gtcagaaatc gtctcatggt atcctcaatg
     4261 ggcctgaatt agtctttatc gattcgactg gctaccgcgc cctgctggtt gcgatatttc
     4321 gcatcttcac ggcggttgta aggtcgcgcc gccgggccgg aaagcggctc aaagctcagc
     4381 gcaccaatta acatgcccgg acgcagcgcc agcggcagct taccggagtt gtagaactcc
     4441 agcacaatac agccagacca gcccggatcg atacggtgcg cggtgacgtg caccataagc
     4501 cccagacgcg ccagtgagga acgaccgtcc agccatccca ccagatcggc tggcagcgtc
     4561 accgactcca gcgtcaccgc cagcgccagc tctcctgggt gaagataaaa cgcctcgctt
     4621 tcatcgagta cgatctcatc gctcatcacg cggtcaagcg cggcgctcac ttcatctttg
     4681 ggaccgctca gatcgataaa cgcagccgtg tgaccacgga aggtacgaaa tttattgccc
     4741 aggcgcacat ccaccgtcgc gccgttaata cgctccactg gcggacgtgg gttgatcgac
     4801 aaacggcctt catcaagcca ggcttcaata tctcggtcac acagacgcat ggcattttct
     4861 cctttcgcgc atcactccct taacgccaat tacgtcaagg gcataccagg ttatcactga
     4921 acggtacaca atttatcaag cttattcaaa gaactgactt attttcgctt tcaatatatc
     4981 gatggcgatg cggtttttcc cgccgcgcgg cacgataatg tccgcatatt gtttagaagg
     5041 ctcaatgaat tgcaggaaca tcggacgcac ggttttttga tattgcgcca tcactgaatc
     5101 cattgaacgc ccacgctcgt taacgtcacg cttgatgcgg cgcatcaggc agatatccag
     5161 cggggtatca acgaaaatgg agaagttaag ttcatcacgc aaacgcgcat ccgtcagcag
     5221 caaaatgcct tcgagaatga tgactttctt cggctcaacc gtcaccgttt ctttcatacg
     5281 cgtatgttca acatagctgt aaaccggcag gtcaatggcc gagccgcgtt tcaacgcttg
     5341 taaatgctca agcaacaggc tgtgatccat agcgctggga tggtcgtagt tggtcttaac
     5401 gcgttcttcc atcgacagat ggctttgatc tttgtaatag cagtcttcgg gaattacgcc
     5461 gatgtgttca tcaccgactt gctcacgtaa ttcacgataa agggtactgg caataagact
     5521 cttgccggaa gccgatgcgc cagcgatacc gataatgacg cactgatgag actgatcagt
     5581 catatattta gcgacctgat taacctggat gttaggaagg gggcgacgaa gcgccaaacg
     5641 cggcaattat agggatttca tccgcctgat accagtcgaa tagcgttgcc gcgcgctcag
     5701 agttaattgt tgacgaagaa ttcccggtgg caaattacgt tgatcagttt tatacaaggt
     5761 aaaaattgtt atacgcagtt gcgcaaatta tccgccttta cgtcacttta tgagcaattc
     5821 gcatataaaa tgtaaaactt ttgtactagc ataaacacag aaacgaatac tggcgacctg
     5881 gtcttgcgga taaagcggta atgagcaaac aatcacagca tgtattaatt gccctgcccc
     5941 acccgctgct tcacctggtc agtttaggtt tagtctcgtt tatctttacc cttttctcgc
     6001 ttgagctttc gcagtttggc acccaactcg ccccactgtg gttcccgacg tccatcatga
     6061 tggtggcgtt ttatcgccat gccgggcgca tgtggccggg gattgcgctg agctgctcgc
     6121 tgggaaatat cgccgcatcc atcctgcttt tttccaccag ctcgctgaac atgacctgga
     6181 cgaccatcaa tattgttgaa gccgtggtcg gggcagtgct actgcgtaaa ttgctgccgt
     6241 ggtataaccc attgcaaaat ctggctgact ggctgcgtct ggcactcggc agcgccattg
     6301 ttccacctct gttagggggt gttctggtta tcctgctgac gcccggagac gatcctctca
     6361 gggcattttt gatatgggta ctgtcagaat ccatcggcgc actggcactg gtgccgctgg
     6421 gattgttatt taaaccacac tatctgctgc gccatcgcaa cccacggttg ctttttgagt
     6481 cgctgctcac gttagccatc acactgacgt taagctggct ttcgatgctg tatctgccgt
     6541 ggccttttac tttcattatt gtgctgttga tgtggagcgc cgtgcgcctg ccacgaatgg
     6601 aagccttttt gatcttcctt accacggtga tgatggtgtc actgatgatg gccgcggatc
     6661 cctccctgct tgctacgccg cgtacatacc tgatgagcca tatgccgtgg ctaccgtttt
     6721 tgctgatcct gctgcccgcc aacatcatga ccatggtgat gtatgccttt cgtgcggaac
     6781 gcaaacacat ttccgaaagc gaaacccgct ttcggaacgc gatggaatat tccgccatcg
     6841 gcatggcatt agtgggcacc gagggacaat ggctgcaatc caacaaagcg ctctgccagt
     6901 ttctcggtta cagtcaggaa gagctgcgcg gactcacctt tcagcaactg acctggccgg
     6961 aggatctcaa taaagatctg caacaggttg aaaagctgat cagcggtgaa ataaacacct
     7021 attcaatgga aaaacgctac tacaaccgca atggcgatgt tgtctgggcg ttgcttgccg
     7081 tctcactggt gcgccacacc gatggtacgc cgctctattt tatcgctcag attgaagaca
     7141 ttaacgagct aaaacgcacc gaacaggtga accagcaact gatggagcgc atcacgctgg
     7201 ctaacgaagc gggcgggatt ggcatctggg agtgggagct aaagccgaat atttttagct
     7261 gggataagcg gatgttcgag ctgtatgaaa ttcctccgca tatcaaaccg aactggcagg
     7321 tgtggtacga gtgcgtactg ccggaagatc gccagcatgc cgaaaaagtg attcgtgatt
     7381 cgttgcaatc acgctcgccc tttaagctgg aatttcgcat tgccgtgaaa gatggcatcc
     7441 gccatatccg cgccctcgcc aaccgggtac tgaataaaga aggcgaagtc gaacgcctgc
     7501 tcggcattaa tatggatatg accgaagtga aacagcttaa cgaggcattg tttcaggaaa
     7561 aagagcgcct gcacattacg ctggattcca tcggcgaagc cgtggtctgt attgatatgg
     7621 caatgaaaat tacctttatg aatccggtgg cggagaagat gagcggctgg acgcaggaag
     7681 aagcgttagg tgttccgctc ctgacggtgt tgcatattac ttttggcgac aacggaccat
     7741 taatggagaa catttacagt gccgacacct cacgttccgc gattgaacaa gatgtggtgt
     7801 tgcactgtcg gagcggcggc agctacgacg tgcattacag tattacgccg ttaagtactc
     7861 tggacggcag caatataggc tcggttctgg tgattcagga cgtcaccgaa tcgcgtaaaa
     7921 tgctgcgcca gctgagctac agcgcctccc atgatgcact gacgcatctc gccaatcgcg
     7981 ccagttttga gaagcaacta cgcatcctgc tgcaaacggt aaacagtaca catcagcgac
     8041 atgcactggt gtttatcgat cttgatcgct ttaaagcggt gaatgacagc gccgggcacg
     8101 ccgctggcga cgctttactg cgcgaactgg cgtcattgat gctgagtatg ctgcgctcca
     8161 gcgacgtgct ggcgcgactc ggtggcgatg aatttggtct gctgctgcca gattgtaatg
     8221 tcgaaagtgc gcgttttatc gctacacgca ttatcagcgc cgtgaatgac tatcacttta
     8281 tctgggaagg acgtgtacat cgggtaggtg ccagtgccgg gattaccttg attgatgaca
     8341 acaatcatca ggcggctgaa gtgatgtcgc aggctgatat cgcctgttat gcctccaaaa
     8401 atggtggacg gggccgggtg acggtttacg aaccgcagca agctgccgca catagcgaac
     8461 gggcggtgat gtcgcttgat gaacagtggc ggatgattaa agagaatcag ttgatgatga
     8521 tcgcccacgg tgtcgcttcg ccgcggatcc cggaagcgcg taatttgtgg ctgatttcac
     8581 ttaagctctg gagttgcgaa ggcgagatta ttgatgaaca aacatttcgt cgtagcttca
     8641 gcgatccggc acttagccat gctcttgacc gacgggtatt ccacgatttt ttccagcagg
     8701 ccgcaaaagc gattgccagt aaaggcttaa gcatcgccct ccccctttcc gttgccggtt
     8761 tgagtagcgc cacgctggtg aatgaactgc ttgagcagct ggaaaatagc cctctacccc
     8821 cacggttatt acatctgatt attccggcag acgcgatttt agatcacgca gaaagcgtgc
     8881 aaaaactgcg gctggcggga tgtcggatcg tattcagtca ggtgggccgc gatctgcaaa
     8941 tcttcaactc gctgaaagcg aatatggcag attacctgct acttgatggt gagttatgcg
     9001 ccaatgtgca gggaaatttg atggatgaga tgctgattac gatcattcag gggcacgctc
     9061 agcggctcgg gatgaagacc atcgccgggc cagtagtttt acccctggtg atggatacgc
     9121 tttctggcat cggcgtcgat ctgatttatg gcgatgtgat tgccgatgcc caaccgctgg
     9181 atttgctggt gaatagcagt tatttcgcga ttaactgagg cgtgcttctc catcgcctga
     9241 tacgacgcta acgcgtctta tcatgcctac aaatcgctca ttccccaggc cggataaggc
     9301 gctcgcaccg catccggcga ccaacgtcat gcttcgtctg gttgccagcc ttccgtatac
     9361 cagatatgca acagcgcata agaacgcaag ggcttccagc gttcggcata acggcggatt
     9421 tgtgccggtg tcattcccgg aaatcgctgt ttaatcagat aatcatccgg caggaaaaca
     9481 tctttcgcct gccagccgcg caaggcgaaa taattcgccg tccagcgccc gatacccgga
     9541 aaagtttgca gcgttttcat cgcctgctcc acatcgcccg gtattgtcat tggtaaggtg
     9601 ccctccagcg ccgcatttgc cagatgaatc agcgcctctg cccgtttcaa cggcatacct
     9661 aacgctttta atgcctgcgg atctgctgct gccagccgct gaggcgtggg gaagcagaca
     9721 taatccggaa aatcatccag cctttcgcca taaagctgtg ccactctggc ggtcaatttt
     9781 gccgccatcg ccacgctcac cagctggcct aaaatcgccc gcacgccctg ctcaaaagca
     9841 tcaacacaac cgggtaaacg caatccgggc cgtgccgcgc ctaacttgcc caacgcaccg
     9901 ttaacaatct gcgggttaca ttgcagatca aacaggcggc tcattttcgc cagacactct
     9961 gcggcaacgg gttctaaacc tgcacttaaa tttatgtgca gagtatggct ggctatatcc
    10021 ggaatagcag tcaccacgcc gcgacattcg cccaccgcca gactacgggc ataataatcg
    10081 tcagcgaccg tttccacacc gttcaccgca cgggcggcga gaaatcccaa catccacgac
    10141 cagtcatacg gcggctgcca gttcagggta tacatcgcat ctccttgtta atccgctttc
    10201 agcataaacg ttattcagac gctacgcttt gctttcatat tccggttgtc gcgacggcaa
    10261 catttcgcta aagtcacgcc ccttcttcac cggcatgggg attatttcgt gtttattggt
    10321 tttgattacg gtacagcaaa ctgttcagtg gcggtcatgc gcgacggtaa gccgcaatta
    10381 ctgaaaatgg aaaacgacag cacgctgctg ccttcaatgc tttgcgcgcc aacgcgtgaa
    10441 gcggtaagcg aatggctgta ccgccatcat gatgttccgg cagacgacga tgaaacgcag
    10501 gcgctgttgc gtcgggcgat tcgttataac cgcgaagaag atatcgatgt tacggcgaaa
    10561 agcgtgcagt tcggtctttc ctcactggca cagtacattg atgatccaga agaggtgtgg
    10621 tttgtgaaat caccaaaatc attcctcggt gccagcggct taaaaccgca gcaggtagcg
    10681 ctgtttgagg atctggtctg cgcaatgatg ttgcacattc gccagcaggc gcaggcgcag
    10741 ctgccggagg caattactca ggcggtgatt ggtcgcccga tcaacttcca ggggctgggc
    10801 ggcgatgaag caaacgccca ggcgcaaggg attctggaac gcgcggcgaa gcgtgccggg
    10861 tttaaggatg tggtattcca gtacgagccg gtcgcggctg ggctggatta cgaagccacc
    10921 ttgcaggaag aaaaacgggt gctggtggtg gatatcggcg gtggtacgac tgactgttca
    10981 ttgctgctga tggggccgca gtggcgttcg cgtctcgatc gtgaagccag cctgctgggt
    11041 cacagtggtt gccgtattgg cggtaacgat ctggatatcg cactggcgtt taaaaacctg
    11101 atgccgttac tgggcatggg tggcgaaacc gaaaaaggca tcgccctgcc gattctgccg
    11161 tggtggaatg cggttgccat caacgacgtt cctgcgcaaa gtgatttcta cagtagtgcc
    11221 aacggtcgtc tgcttaacga cctggtacgc gatgcccgcg aaccggaaaa agtggccctg
    11281 ttacagaaag tctggcgtca gcgtttaagc tatcgcctgg tacgtagcgc agaagagagc
    11341 aaaattgcgc tttcaagcgt agcggaaacc cgcgcctcac tgccgtttat cagcgatgaa
    11401 ctggctacgc tgattagcca gcaagggctg gaaagcgccc tcagccagcc actggcgcgg
    11461 attctggaac aggtgcaact ggcgctggat aacgcccagg aaaaaccgga tgttatctat
    11521 ctgaccggcg gtagcgcccg ttcaccgctg attaaaaaag cgctgacaga acagttgccg
    11581 ggcattccga ttgcaggtgg cgatgacttt ggctccgtca ccgccgggct ggcacgctgg
    11641 gcggaagtgg tgtttcgtta attgaaaact ggctcagcaa cggcttaaat cagcctgcgc
    11701 ctgagccagt ttttctgccg cctgacgtag cggttccatc aacggcatgg tccgtgctgg
    11761 agcatgaagg cggaatcgct gcaactctgt cgcaccgaca ttcagcgccg attccagccg
    11821 atgtcgtttg gcggccattt ttgccattac cgcttgtctg tctgcaggcg ttaccgcttc
    11881 gttcggcctg aacacaaaac ggcgttcaca gctcgctttc cagtcgataa ccgcctgggt
    11941 cagatgatca ccaaaacctt tcacttgctt aacgctccgt cgggtaacat ccgctgctgt
    12001 ttcaatacca aaggaccgta acgccgcttt acgcgcaggg ccaacgccgg gaatagaggc
    12061 aacatcaata aaaaatccct ccagaaactt ctgcttctgc cgttcccttg cggtgtcctg
    12121 aagtgctgcc agatcgcgct tttcttcttc cggtaaccca agaatttcgt ccttcatttt
    12181 ttcgagcatc gtccgtttgg cgataaaacc ttccagcccg cccaactgct ggatctggct
    12241 gactaaatgg tcatagtcca ttttcgcgcg attaaacgcc tcacggcgtt gctggatttc
    12301 tgctttgtac gctttgcttg tcaggctgcc aataatccag atagccgcca gcacaggaac
    12361 caaaataata taacgtggtt ctgtctgaag gccgcaaagc agcagcgata acgctgacag
    12421 tgcgatctca atcagaatga tgtattcacg ccttaacagg cctaaaggaa gcggcctgcc
    12481 agtcggttgg aaatgatcgg gtaatggtaa ttgcaatgct ggcggtgcta ctgacgccat
    12541 caccatcgcc cagactttcg ccagcacaaa atcaccgctg gtggtaatga cctcttcgcc
    12601 gagatcaata aaatagataa cgccttgatt atccagagca caccacgggc agtcagccaa
    12661 atgaccggga taaacatgca ttgccgaaac ggtacatttc tttaactgtt ggcgtagtaa
    12721 atcaagtgcc gctacccacg cttttgccgt cggacgcccg gttgccacgc cactttccgt
    12781 gaatgcctgc tgaaacatgg cttcaacatg cccggtaaca tcgacagcgg aatagatcgt
    12841 ggcggcggtt ttaaaccacg tcgctgatta tctgacgcgt aggcataacg gaaatgggca
    12901 atatccgtct ccagtgcatt acccgcatca gagacaagcg gtacaccgga ataaggatgc
    12961 cgcccaccaa acaagacgtg aaaaatcagc aacgcaaggc caaaattatc gtgattcgcg
    13021 gtgcgttcaa agccgacaaa tgatggcaag gtttgttgct ctggcggcgt aaaatgcgac
    13081 acgccgactt cgcataaatg cagcgtgcca ttggcgttaa tctgaaagga gtcgctatca
    13141 atcaacacca ctttgctgtc gcgacctacc ataaagctgt tctggtttac gtcacccacg
    13201 acgtgcccgt gctcgtgaat cgtagcaaaa gatgaagcaa tattgcgcgc aacatagagt
    13261 agaaaatccc acgcacaatg agggtaacgt tgacgacgat gtgccgggct atagatcata
    13321 tgaatcggtt ctttaccaga aacttttggc atcataaaac cgattacttt tccgcctcgt
    13381 ccaccgtgaa gcgttgcctg cggccaggcg acataattca acaactgcgc gtcagctgtc
    13441 gcagccataa aggcaagttt gtcctgtttt aaggcgggtg gcggcgtgtg ataaatcttg
    13501 gcgacgctat cgacaaactc ctcgatatca taaaccgcgc cttcaccgcc tttgcccagt
    13561 tcacagcctg gcgtaacgca ctcaccagta gcagtatata aagtgggttt catggtaacc
    13621 acagcgccag tgccaacgtt ttatcatcat cggtacgctc gtttaccgcc gggccggaca
    13681 aaaactgctt taacaattca ggtaataaat cgagttgttc ctgcgttgct gacgccagcc
    13741 cattgaaaaa cggggtaaag aaaggcacat gcggagaatt atccagcata tttaacgcca
    13801 gccgctggat accatccgta aatgctgcaa ctttgtgcgc acgctcagtg ctggtgaaag
    13861 tttccagtct ggaaacggca tcttcatcgg taatgaagtg cgtcatattg gtatattcac
    13921 ccaccatcgg cgtgagcggc agttgcaaac cgtgccccaa atctaccacc acgccgccat
    13981 cgccaatctg cataatcaag gtgccattag ctgatgaaat caatcccaaa aacgtgcagg
    14041 caaaatcgcg aaccgctaat tctttggctt ccgcctcggc aaaaagtcgc tgacgaatgg
    14101 tcagcacaat gtccgttgcg agaacatcat taagccccaa ttccccgccc tgcacttttt
    14161 gcgacatata agccatcgct tcattgacgg cgagcatcgc accttcgcct ccctgcgagg
    14221 cgctacctgc gccatcagcg acaaataccg acaataatgg ctgctggtca tttaaccagg
    14281 caacctgcat ctggcaggca tcctggcaag gcaagtccgc gctgatgtgc gacgttccaa
    14341 cggttgatgc atagaccaga cgccagctca cactgacgtc cagccttttg gcgcttccag
    14401 cacaacttcc gttcccggcg tggagcggga aaccgaacgc agcgaactgg ataaccagct
    14461 aaatagttcg cggaattgta atccttgaag aggcaaaggc tgacgaacgc tgatttgggc
    14521 cagcgtcttc atatccgccc cctgaacacc gatggaaaag aaggcaaact tcttatcttc
    14581 ttccccctga aacaccttgt tggcagcagc ctgccactca tcggttggtg cgccatcggt
    14641 aatcaggaaa atccacggac gataatagga aataccattg gcgcgatact cacgtttgcg
    14701 ctcctcaacc atatccaggg ctttggtaat ggcggccccc attggtgtat cgccctgggc
    14761 aaacaggatg ggcgggaaaa aattagcggc gctggtaaat ggttgttcca catgcaccgg
    14821 gccgaacgtc acaatgccga gttccactct ttttaaggcc agcggatcgg caagcagctc
    14881 atcgcgaaag gtaaccaatc cggcgttaag ttcattaata ggtctaccgt tcatagaacc
    14941 agaaacatcc aacagcaaaa tgcaggggca acgtggttcc gggttactgg caaaatcgct
    15001 ggtggcaaac gtgatttgtt cgctcataag aaatcctttc ttagagaatt attgaggccc
    15061 ggtatcatac cgactgcaac aatgaaataa gagcaaacga agaaactttc agaattaggc
    15121 ccggcggcgc agaaaatttt gctattcgcc tcgcaagaac ggagagccgt cgattgacac
    15181 taagggcgga gtgacataat ttcaggagtg agggttaggg agaggtttcc ccctccccct
    15241 ggtgttctta gtaagcctgg aagctaatca ctaagagtat caccagtatg atgacgtgct
    15301 tcatcataac cctttcctta ttaaaagccc tcttctccgg gagagggttt cccgtttcag
    15361 cgtcccgctg aaatcgtcgg cttacctcct ttcgccctga atgcagtcta tcgctaacgc
    15421 gtgaacaaaa tagtttcctc tgtgattatt gcgtaacgac tcgcaaagaa tatttgctgg
    15481 caggatcgca gactacaaag cctgcggatt gacaatctta tcgtgaaggc ataatttcag
    15541 gagtgagggt agagcggggt ttcccccgcc ctggtagtct tagtaagctg gccagctaat
    15601 aactaagagc accacgatga tgagtagctt catcatgacc ctttccttat ttaaggcccc
    15661 ttcctcggga ggggctttcc cgtttcagcg tcccgctgaa atcggcggcc tacctccttt
    15721 cgccatgcaa gcagtctatc gctaacttgc agataaaata gtttcacatg tgattattgc
    15781 gtaacgtctc gcaaagaata tttgctggca ggatcgcaga ctacaaagcc tgcggattga
    15841 caatctaatt gtgaaggcat actttcagga gtgagggtag agcggggttt cccccgccct
    15901 ggtagtctta gtaagccggg aagcttatga ctaagagcac cacgatgatg atccacttca
    15961 tcatgaccct ttccttattt aaggcccctt cctcgggagg ggctttcccg tttcagcgtc
    16021 ccgctgaaat catcggctta cctcctttcg ccctgaatgc agtctatcgc tgacttgcgg
    16081 ataaaatagt ttcacatgtg attattgcgt gacgtctcgc aaagaatatt tgctggcagg
    16141 atcgcagact gcaaagcctg cggattgaca atcggaccgt gaaggcatac tttcaggagt
    16201 gagggtagag cggggtttcc cccgccctgg tagtcttagt aagctgggta gctaacgact
    16261 aagagcacca cgatgatgag tatcttcatc atgacccttt ccttatttaa ggccccttcc
    16321 tcgggagggg cttgcccgtt tcagcgtccc gctgaaatcg tcggcctacc tccttttgcc
    16381 ctgaatgcag tctatcgcta acgcgtagat aaaatagttt cctgtgttat tactggatgg
    16441 gtgctcgcaa atgtgcccgc cattcagacg attcccgata gtgtttcata attcctccat
    16501 ttttctccct gattggctgg ctacactagt atcattccgc gaatcgtttc aggaagagaa
    16561 actcttaacg atgaaaagca gttataaatc ccgttgggta atggtaatcg tggtggttat
    16621 cgccgccatc gccgcattct ggttctggca aggccgcaat gactcccgga gtgcagcgcc
    16681 aggggcgacg aaacaagcgc agcaatcgcc agcgggtggt cgccgtggta tgcgttccgg
    16741 cccattagcc ccggttcagg cggcgaccgc cgtagaacag gcagttccgc gttacctcac
    16801 cgggcttggc accattaccg ccgctaatac tgttacggtg cgcagccgcg tggacggtca
    16861 actgatggcg ttgcatttcc aggaaggcca gcaggtcaaa gcaggcgatt tactggcaga
    16921 aattgacccc agccagttca aagttgcatt agcacaagcc cagggccaac tggcaaaaga
    16981 taaagccacg cttgccaacg cccgccgtga cctggcgcgt tatcaacaac tggtaaaaac
    17041 caatctcgta tctcgtcagg agctggatgc ccaacaggcg ctggtcagtg aaaccgaagg
    17101 caccattaag gctgatgaag caagcgtcgc cagcgcgcag ctgcaactcg actggagccg
    17161 catcaccgca ccagtcgatg gtcgcgttgg tctcaagcag gttgatgttg gtaaccaaat
    17221 ctccagtggt gataccaccg ggattgtggt gatcacccag acgcatccta tcgatttgct
    17281 ctttaccctg ccggaaagcg atatcgctac cgtagtgcag gcgcaaaaag ccggaaaacc
    17341 gctggttgtt gaagcctggg atcgcaccaa ttcaaagaaa ttaagtgaag gcacgctgtt
    17401 aagtctcgat aaccaaatcg atgccactac cggtacgatt aaagtgaaag cacgcttcaa
    17461 taatcaggat gatgcgctgt ttcccaatca gtttgttaac gcgcgcatgt tagtcgacac
    17521 agagcaaaac gctgtggtga tcccaacagc cgccctgcaa atgggcaatg aaggccattt
    17581 tgtctgggtg ctgaatagcg aaaacaaggt cagcaaacat ctggtgacgc cgggcattca
    17641 ggacagtcag aaagtggtga tccgcgcagg tatttctgcg ggcgaccgcg tagtgacaga
    17701 cggcattgat cgcctgaccg aaggggcgaa agtggaagta gtggaagccc agagcgccac
    17761 cactccggaa gagaaagcca ccagccgcga atacgcgaaa aaaggagcac gctcctgatg
    17821 caggtgttac ccccgagcag cacaggcggc ccgtcgcgcc tgtttattat gcgtcctgtc
    17881 gccaccacgc tgctgatggt ggcgatctta ctcgccggga ttatcggtta tcgagccctg
    17941 cccgtttcgg cgctgccgga agtggactat ccgaccattc aggtagtcac gctctaccca
    18001 ggtgcgagcc cggatgtcat gacctctgcc gttaccgcgc cgcttgaacg ccagtttggg
    18061 cagatgtctg gcctgaaaca gatgtcgtcg caaagttccg gcggtgcgtc agttatcact
    18121 ttgcagttcc agctaacatt accgctcgat gtcgccgagc aggaagtgca ggccgcgatt
    18181 aacgctgcga ccaacttgtt gccgagcgat ctgcctaacc cgccggttta cagcaaagtg
    18241 aacccggcag atccgccgat catgacgctc gctgtcacct caaccgccat gccgatgacg
    18301 caagtggaag atatggtgga aacccgcgtc gcgcagaaaa tctcgcagat ttccggcgtc
    18361 ggcctggtga cactttccgg cggtcagcgt ccggctgttc gcgtcaaact taacgctcag
    18421 gcgattgccg ccctcggcct gaccagcgaa accgtgcgca ccgccattac cggcgctaac
    18481 gtcaactcgg caaaaggtag cctcgacggc ccttctcgtg cggttacgct ttccgctaac
    18541 gaccagatgc aatccgccga agagtatcgc cagctaatca tcgcctacca gaacggcgcg
    18601 ccgattcgtc tgggcgatgt cgcaaccgta gagcaaggtg cagaaaacag ctggcttggc
    18661 gcatgggcta acaaagaaca ggccattgtg atgaatgttc agcgccagcc cggtgctaac
    18721 attatctcca ccgccgacag cattcggcag atgctgccac agctcaccga gagtctgccg
    18781 aaatcggtga aggtgacggt actttctgac cgtaccacca atattcgcgc atccgtggat
    18841 gatactcagt ttgaattgat gatggctatc gcgctggtag tcatgattat ctacctgttt
    18901 ttgcgtaata ttccggcgac catcattccc ggcgtcgcag tgccgctgtc gttaatcggc
    18961 accttcgcgg tcatggtgtt tctcgatttt tcaatcaata acctgacgct gatggcgtta
    19021 actatcgcca ccggatttgt ggtcgatgac gccatcgtgg tgatcgaaaa catttcccgc
    19081 tatatcgaaa aaggcgaaaa accgctggcg gcggcgctca aaggcgcagg tgaaatcggt
    19141 tttaccatta tctcgctgac cttctcgctg attgcagtgc tgatcccgct actgtttatg
    19201 ggcgatatcg tcgggcgact gttccgcgaa tttgctatta ctctggcagt cgcgattttg
    19261 atctcagccg tggtgtcgct gaccctgaca ccgatgatgt gtgcgcggat gctcagccag
    19321 gagtcgctgc gtaaacagaa ccgcttctcc cgtgcctcgg aaaaaatgtt cgacaggata
    19381 atcgccgcct atggtcaggg acttgcgaaa gtgctgaatc atccgtggct gaccttaagc
    19441 gtggcgctca gcacgctgct gcttagcgtg ctgttgtggg tgttcattcc gaaaggtttc
    19501 ttcccggtac aggacaacgg cattattcag ggcactttgc aggcaccgca atccagctcc
    19561 tttaccaata tggcccagcg gcaacgccag gtcgcggatg tgattttgca agatccggcg
    19621 gtgcaaagtc tgacctcatt tgttggcgtt gatggcacta acccgtcgct gaacagtgcg
    19681 cgtttgcaga tcaacctcaa accgttggat gaacgtgatg atcgggtgca aaaagtcatc
    19741 gcccgcctgc aaacggcggt ggataaagtg ccgggcgtcg atctcttcct gcaaccaacg
    19801 caggacctga ccatcgatac tcaggtcagc cgcacccagt accagtttac cttgcaggcc
    19861 acgtcactgg atgcgctcag tacctgggtg ccacagttga tggaaaaact ccagcaactg
    19921 ccacagcttt ctgatgtctc cagcgactgg caggacaaag ggctggtggc gtatgtcaat
    19981 gttgatcgcg acagcgccag ccgtctgggg atcagcatgg cggacgtcga taacgccctt
    20041 tacaacgcgt ttggtcagcg actgatttcc actatttata ctcaggccaa ccagtatcgc
    20101 gtggtgctgg agcacaacac cgaaaatact cctgggcttg cggcgctgga tactattcgc
    20161 ctgaccagca gcgacggcgg cgtggtaccg ctaagctcaa ttgccaaaat tgagcagcgt
    20221 tttgcgccgc tttccatcaa ccatctggat cagttcccgg taacgaccat ctcctttaac
    20281 gtgccggata actattcgct gggcgatgcg gtgcaggcga ttatggacac cgaaaagacg
    20341 ctgaatctgc cggtggatat caccacgcag ttccagggca gcaccctcgc cttccagtcg
    20401 gcgttgggca gcactgtctg gctgattgtc gcggcggtgg tggcgatgta tatcgtgctc
    20461 ggcattctgt acgagagctt tattcacccg atcaccattc tctcgacgct acccaccgca
    20521 ggggtcggcg cactgctggc gttactgatt gccggtagcg agctggatgt gattgcgatt
    20581 atcggcatta ttttgctgat cggtatcgtg aagaagaacg ccatcatgat gatcgacttc
    20641 gcgctggctg ctgagcgcga gcaaggcatg tcgccgcgcg atgcaatcta ccaggcttgt
    20701 ctgttgcgtt ttcgtccgat cctgatgacc accctggcgg ctctgcttgg cgcgctgccg
    20761 ctgatgttga gtaccggggt cggcgcggaa ctgcgtcgtc cgttaggtat cggcatggtc
    20821 ggcggtctga ttgtcagcca ggtgctgacg ctgttcacca cgccggtgat ttatctgcta
    20881 ttcgaccgcc tggcattgtg gaccaaaagc cgctttgctc gtcatgaaga ggaggcgtaa
    20941 gtgaagtttt ttgccctctt catttaccgc ccggtggcga cgattttact gtcggtcgcc
    21001 attaccctgt gcggcatact gggtttccgt atgctgccag tcgccccgct gccgcaggtc
    21061 gattttccgg tgattatggt cagcgcctcg ctgcccggtg cgtcaccaga aacaatggcg
    21121 tcttccgttg ccacgccgct ggaacgttcg cttggacgca ttgccggggt cagtgaaatg
    21181 acctccagca gttcgctcgg cagcacgcgc attattttgc agtttgattt tgaccgcgat
    21241 atcaacggcg cagcgcgcga tgtgcaagcg gcgatcaacg ccgcgcaaag tttgctgccc
    21301 agcgggatgc caagccgtcc gacctatcgc aaagcgaacc cgtcggatgc gccaattatg
    21361 atcctcacgc tgacatccga tacttattcg cagggtgaac tgtacgattt cgcctcgacg
    21421 cagctggctc cgacgatttc gcaaatcgac ggtgtcggtg atgtcgatgt tggcggcagc
    21481 tcactgcccg ccgtacgcat cgggctgaat ccgcaggcgc tgtttaatca gggcgtgtcg
    21541 ctggacgatg tgcgcagcgc catcagcaat gccaacgtgc gtaagccgca gggggcgctg
    21601 gaagatgata cccatcgctg gcagatccag actaacgatg aactgaaaac cgccgccgaa
    21661 tatcagccgt tgattattca ttacaacaac ggcggcgcgc ttcgtctggg cgatgtggcg
    21721 acggtgaccg actcggtgca ggatgtgcgc aacgccggga tgaccaacgc caaaccggct
    21781 attttgctga tgatccgcaa gctgccggaa gccaatatta tccagacggt tgacagcatc
    21841 cgggcaaaat taccggagtt gcaggagacc attccggcgg cgattgatct gcaaattgcc
    21901 caggatcgct cccccaccat tcgcgcctcg ctggaggaag tcgagcaaac gctgattatc
    21961 tcggtggcgc tggtgattct ggtggtcttt ttattcctgc gctcgggtcg cgccactatt
    22021 attcccgccg ttgcggtgcc ggtttcgctg attggtacgt ttgcggcgat gtacctgtgc
    22081 ggattcagtc tcaataacct ttcgttaatg gcgctcacca tcgctactgg tttcgtggtg
    22141 gatgacgcca tcgtggtgct ggaaaacatt gcccgtcacc tggaagcggg gatgaaaccg
    22201 ctacaggccg cgctgcaagg cactcgcgaa gtcggtttta cggtgctgtc gatgagtctg
    22261 tcactggtgg cggtgtttct gccgctgctg ttgatgggcg gattgccggg tcgcctgtta
    22321 cgcgagtttg ccgtgacgct ttctgtcgcc attggtattt cgctgctggt ttctctgacg
    22381 ctaacgccaa tgatgtgcgg ctggatgcta aaagccagta agccgcgcga gcaaaagcga
    22441 ctacgtggtt ttggtcgcat gttggtagcc ctgcaacaag gctatggtaa gtcgctgaaa
    22501 tgggtgctta atcatacccg tctggtgggc gtggtgctgc ttggcaccat tgctttgaat
    22561 atctggctgt atatctcgat cccgaaaacc ttcttcccgg agcaggacac cggcgtgttg
    22621 atgggcggga tacaggcgga tcagagtatt tcgtttcagg cgatgcgcgg taagttgcag
    22681 gattttatga aaattatccg tgacgatccg gcagtggata atgtcaccgg atttaccggc
    22741 ggttcgcggg tgaacagcgg gatgatgttt atcacgctga agccacgcgg cgaacgcagc
    22801 gaaacggcgc agcaaattat cgaccgtctg cgcgtgaagc tggcgaaaga accgggggcg
    22861 aatctgttcc tgatggcggt acaggatatt cgcgttggcg gacgtcagtc gaacgccagc
    22921 tatcagtaca cgttgctgtc cgacgacctg gcggcactgc gcgaatggga gccgaaaatt
    22981 cgcaaaaaac tggcgacatt gccggaactg gcggacgtga actccgacca gcaggataac
    23041 ggcgcggaga tgaatctggt ttacgaccgc gacaccatgg cacggctggg aatcgacgtg
    23101 caggccgcca acagcctgtt aaataacgcc ttcggtcagc gacaaatctc gaccatttac
    23161 cagccgatga accagtataa agtggtgatg gaagtggatc cgcgctatac ccaggacatc
    23221 agtgcgctgg aaaaaatgtt cgttatcaat aacgaaggaa aagcgatccc gctgtcgtat
    23281 ttcgctaaat ggcaaccggc gaatgctccg ctgtcggtga atcatcaggg attatcggca
    23341 gcctcgacca tctcgttcaa cctgccgacc gggaaatcgc tctcggacgc cagtgcggcg
    23401 atcgatcgcg caatgaccca gcttggtgtg ccttcgacgg tgcgcggtag ttttgccggc
    23461 acggcgcagg tgttccagga gacgatgaac tcgcaggtga tcctgattat cgccgccatc
    23521 gccacggtgt atatcgtgct gggtatcctt tacgagagtt acgtacatcc gctgacgatt
    23581 ctctccaccc tgccctcggc gggcgttgga gcgctgctgg cgctggagct gttcaatgcc
    23641 ccgttcagcc taatcgccct gatagggatc atgctattaa tcggcatcgt gaagaaaaac
    23701 gccattatga tggtcgattt tgcgcttgaa gcccaacggc acggtaacct cacgccgcag
    23761 gaagctattt tccaggcctg tctgctgcgt tttcgcccga ttatgatgac taccctggcg
    23821 gcgctgtttg gcgcgctgcc gctggtattg tcgggcggtg acggttcgga gctgcggcaa
    23881 cccctgggga tcaccattgt cggcggtctg gtcatgagcc agcttctgac gctgtatacc
    23941 acgccggtgg tgtatctctt tttcgaccgt ctgcgactgc gtttttcgcg taaacctaaa
    24001 caagcggtaa ccgagtaaat gacagatctt cccgacagca cccgctggca attgtggatt
    24061 gtggctttcg gcttctttat gcagtcgctg gacaccacca tcgttaacac cgcgcttccc
    24121 tcaatggcgc aaagcctcgg ggaaagtccg ttgcatatgc acatggtcat tgtctcttat
    24181 gtactgaccg tggcggtgat gctgcccgcc agcggctggc tggcggacaa agtcggcgtg
    24241 cgcaatattt tctttaccgc catcgtgctg tttactctcg gttcactgtt ttgcgcgctt
    24301 tccggcacgc tgaacgaact gttgctggca cgcgcgttac agggcgttgg cggcgcgatg
    24361 atggtgccgg tcggcaggtt gacggtgatg aaaatcgtac cgcgcgagca atatatggcg
    24421 gcgatgacct ttgtcacgct gcccggtcag atcgggccgc tgcttggccc ggcgctcggc
    24481 ggtctgctgg tggagtacgc atcgtggcac tggatctttt tgatcaacat tccggtgggg
    24541 attattggcg cgatcaccac actgatgtta atgccgaact acaccatgca gacgcggtgc
    24601 tttgatctct ccggcttttt attgctggcg gttggcatgg cggtattaac cctggcgctg
    24661 gacggcagta aaggtacagg tttatcgccg ctggcgattg ctggcctggt cgcagttggc
    24721 gtggtggcac tggtgcttta tctgctgcac gccagaaata acaactgcgc cctgttcagt
    24781 ctgaaactgt tccgtactcg taccttttcg ctgggtctgg cggggagttt tgccggacgt
    24841 atcggtagcg gcatgttgcc ctttatgacg ccggttttcc tacaaattgg ccttggcttc
    24901 tcaccgtttc atgccggact gatgatgatc ccgatggtgc tcggcagcat ggggatgaag
    24961 cgaattgtgg tacaggtagt gaatcgcttt ggttatcgtc gggtactggt ggcgaccacg
    25021 ctgggtctgt cgctggtcac cctgttgttt atgactactg ctctgctggg ctggtactac
    25081 gttttgccgt tcgtcctgtt tttacaaggg atggtcaact cgacccgttt ctcctccatg
    25141 aacaccctga cgctgaaaga tctcccggac aatctggcga gcagcggcaa cagtctgctg
    25201 tcgatgatta tgcaattgtc gatgagtatc ggcgtcacta tcgccgggct gttgctgggg
    25261 ctttttggtt cacagcatgt cagcgtcgac agcggcacca cacaaaccgt ctttatgtac
    25321 acctggctta gcatggcgtt tatcatcgcc cttccggcgt ttgtctttgc cagagtgccg
    25381 agcgatacgc atcaaaatgt agctatttcg cggcgaaaaa ggagcgcgca atgaagttct
    25441 ggcgacccgg tattaccggc aaactgtttc tggcgatttt cgccacctgc attgtcttat
    25501 tgatcacgat gcactgggcg gtacgtatca gttttgagcg cggctttatc gattacatca
    25561 agcatggtaa tgaacaacgg ctgcaaatgc tcggcgatgc gcttggtgag cagtacgccc
    25621 agcacgggaa ctggcgcttc ctgcgtaata acgatcgctt tgtatttcag atcctacgtt
    25681 cactggagca tgataacaac gaagataagc ccggccccgg tatgccacca cacggctggc
    25741 gcacgcaatt ttgggtggtt gatcaaaata ataaagtgct ggttggcccg cgagcaccgg
    25801 ttccacccga cggcacacgg cggcccatta tggtcaatgg tgcggaagtt ggcgcggtga
    25861 tcgcctcccc tgttgaacga ctgacccgta atactgatat caattttgac agacaacagc
    25921 ggcaaaccag ttggctgatt gttgctttat ctaccttatt agcggcgctg gcgacattcc
    25981 cactggcgcg cggtttgctg gctccggtaa aacgactggt ggatggcacg cacaaactgg
    26041 cagcgggtga tttcactact cgcgtagccc ccaccagcga agatgagttg ggcagactgg
    26101 cagaggattt caatcaactc gccagcacac tggagaaaaa ccagcagatg cgccgcgatt
    26161 ttatggccga tatttctcat gaactgcgca cgcctttagc agtactgcgc ggcgaactgg
    26221 aagccattca ggatggtgtg cgtaaattca cgccggagac gctggcttct ttacaggcgg
    26281 aggtcggtac actgaccaaa ctggttgacg atcttcatca gttgtcgatg tctgatgaag
    26341 gcgctctcgc ctatcaaaaa gcaccggtag atttgatccc actgctggaa gtggcgggcg
    26401 gcgcatttcg cgaacgattt gccagccgtg gcctgaaact gcaattttcc ctgccagaca
    26461 gtattaccgt atttggcgat cgcgatcgtt taatgcagtt gttcaataac ttactggaaa
    26521 acagcctgcg ctacactgac agcggcggca gcctgaaaat ctctgccgag cagcacgaca
    26581 aaacggtgcg cctgaccttt gccgacagcg cgccgggcgt cagtgacgat cagctacaaa
    26641 aattgtttga acgtttttat cgcaccgaag gttcccgcaa ccgtgccagc ggcggttccg
    26701 ggctgggact cgcgatttgc ctgaacattg ttgaagcaca taatggccgc attattgctg
    26761 cccattcgcc ttttggcggg gtaagcatta cagtagagtt accgctggaa cgggatttac
    26821 agagagaagt atgaccgagt taccaatcga cgaaaacaca ccgcgtattt tgatcgtgga
    26881 agatgaaccg aagctgggac agttgctcat tgattatctg cgtgccgcga gctatgcgcc
    26941 gacgcttatc agccacggcg atcaggtact gccgtatgtg cgccagacac caccggatct
    27001 gatcctgtta gatctgatgc tccctggcac cgatggcctg acgctgtgcc gggaaattcg
    27061 tcgtttttct gacattccga tcgtgatggt gacggcaaaa atcgaagaga tcgaccgcct
    27121 gctggggctg gagattggcg ccgatgacta catctgcaag ccgtacagcc cacgggaagt
    27181 ggtagcgcgt gtcaaaacca ttttgcgccg ttgtaaaccg caacgcgaac ttcagcaaca
    27241 ggatgccgaa agcccgttga tgatcgacga aggtcgtttt caggcttcat ggcgcggtaa
    27301 aatgcttgac ctgacgcctg cggaatttcg tctgttgaaa acgctctctc atgaaccagg
    27361 aaaagtgttc tcccgcgagc aattgctcaa tcatctttat gacgactacc gcgtagtaac
    27421 tgaccgcacc atcgacagcc acattaaaaa cctgcgtcgc aagctggaag ctctggacga
    27481 agagcagtct tttatccgcg ccgtctacgg tgtcggttat cgctgggaag ccgacgcttg
    27541 ccgcatcgtt tagttttagc gacatgactt tgttagccgg agatggcgtt cagatcagat
    27601 ccggcaacat tatcccacgt atggtcagca aactgacatt atctctccgc cctggcaggc
    27661 accagaaaat cgctcatact ttaatcggta aacagcacct ttagatgctg ttttcgatac
    27721 gcaatttcaa tcaaggagtc attatggctg gttggtttga actcagtaag agcagtgata
    27781 atcaatttcg gtttgtgcta aaagcgggca atggtgagac tatcctcacc agcgagctgt
    27841 atacctcaaa agcctctgcg gaaaagggca tcgcgtcggt gcgtagcaac agcccgcaag
    27901 aagaacgcta tgagaaaaaa acggcaagca acggcaaatt ctatttcaat ctgaaagccg
    27961 ccaatcatca aattatcggc tccagccaga tgtacgccac cgcgcaatct cgtgaaaccg
    28021 gaattgcctc cgttaaagcc aatggcacaa gccagacggt gaaagacaat acgtaattac
    28081 gatgccgggc gcgtgttgtt ggcgtccggc gctttaccag ctccccccta cagcgctaca
    28141 atgcccgccc ttaaagtggg ggcactcccc tggccgcttc atcaggtgaa gcggatctga
    28201 cctgtcatca gaacgagaga attatgttta aaccggaact cctttccccg gcgggaacgc
    28261 tgaaaaatat gcgttacgct ttcgcttatg gcgcagatgc tgtttatgcg ggccagccgc
    28321 gttactccct gcgtgtgcgc aacaacgaat tcaaccacga aaatcttcag ctcggcatca
    28381 atgaagccca cgcgctgggg aaaaagtttt atgtcgtggt caacattgca ccgcacaacg
    28441 ccaagctgaa aacctttatc cgtgacctga aaccggtggt ggaaatgggg ccggatgcgc
    28501 tgattatgtc cgatccaggg ctgattatgc tggtgcgtga gcacttccct gaaatgccga
    28561 tccatctctc ggtacaggct aacgccgtga actgggcgac ggtgaaattc tggcagcaaa
    28621 tgggcctgac ccgcgtgatc ctctctcgcg agctgtcgct ggaagagatt gaagagatcc
    28681 gcaatcaggt gccggatatg gagatcgaga tcttcgttca cggtgcgctg tgcatggcct
    28741 actccggtcg ctgcctgctc tctggctata tcaacaagcg cgacccgaac cagggtacct
    28801 gcaccaacgc ctgccgctgg gagtacaacg tccaggaagg gaaagaagat gacgttggca
    28861 acatcgtaca caagtacgag ccaattccgg tgcaaaatgt tgagccgacg ctgggtatcg
    28921 gcgcgccaac cgacaaagtg tttatgatcg aagaagccca gcgtccgggc gagtatatga
    28981 ccacgtttga agatgagcac ggcacttaca tcatgaactc gaaagatctg cgcgccatcg
    29041 cccatgttga acgcctgacc aaaatgggcg tgcattcgct gaaaatcgaa ggccgtacca
    29101 aatctttcta ctattgcgca cgcaccgcgc aggtttaccg taaagctatc gatgacgccg
    29161 ctgcgggcaa accgttcgat accagcctgc tggaaacgct ggaaggtctg gcgcatcgtg
    29221 gctataccga aggtttcctg cgtcgtcata cccacgacga ttatcagaac tacgaatacg
    29281 gttattcggt ttctgaccgc cagcagtttg ttggtgagtt taccggtgag cgcaaggggg
    29341 agctcgcggc ggtagcggtg aaaaataaat tctccgttgg cgacagcctt gagctaatga
    29401 cgccgcaagg caacattaac tttacccttg agcatatgga aaacgccaaa ggtgaagcaa
    29461 tgccggtcgc accaggcgat ggttatactg tgtggctccc ggtgccgcag gatctcgaac
    29521 tgaattacgc gctgctgatg cgtaatttct ccggtgaaac cacgcgtaac ccccacggta
    29581 agtgattaat ttcgattatt tttcccggat gaaaaattct tagaaaccga tcacatacag
    29641 ctgcatttat taaggttatc atccgtttcg ctgaaaaaca taacccataa aatgctagct
    29701 gtaccaggaa ccacctcctt agcctgtgta atctccctta cacgggctta ttttttatac
    29761 ataaaccata gagacggatt gatcccaggt caccgtcttc cattgaccac atcgatagaa
    29821 tctcccttca tagcacgatg cctttcactt attggcatcg tgctcgcaca ggttccggtt
    29881 acgcacagcc agaacgcgca tgtttgatgc ttaccaaaaa atattctcac tctccacatt
    29941 tgaatgtcag acgagcgacg ccatgtaatc ctgcaccttc tgtcttcagg tcaactatct
    30001 gcattttttt gccctgagta acacagaaat gaactgcatc attttttact atattttctg
    30061 caccagatat tctacccctg gctaaagaag cttcggcttc ggtgtagtat tggttatcga
    30121 gtttacgctg aatattactt ttatatgcaa gaccaaattt accgatactt gtctcatcat
    30181 tatgtacagc acacccagac ataagaaaaa cactaattaa tgatatagca gctatctttt
    30241 tcatctcacc ttcccccatt aaataccaac gacacgctct ggtgtttaaa ta
//
LOCUS       DAZFLD010000032        30156 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.032, whole genome shotgun sequence.
ACCESSION   DAZFLD010000032 DAZFLD010000000
VERSION     DAZFLD010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30156)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30156)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30156
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.032"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..231
                     /gene="tuf"
                     /locus_tag="ACHKBL_001248"
     CDS             <1..231
                     /gene="tuf"
                     /locus_tag="ACHKBL_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312930.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFO5818141.1"
                     /translation="RHTPFFKGYRPQFYFRTTDVTGTIELPEGVEMVMPGDNIKMVVT
                     LIHPIAMDDGLRFAIREGGRTVGAGVVAKVLS"
     gene            461..844
                     /gene="secE"
                     /locus_tag="ACHKBL_001249"
     CDS             461..844
                     /gene="secE"
                     /locus_tag="ACHKBL_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HFO5818142.1"
                     /translation="MSANTEAQGSGRGLEAMKWVVVVALLLVAIVGNYLYRDIMLPLR
                     ALAVVILIAAAGGVALLTTKGKATVAFAREARTEVRKVIWPTRQETLHTTLIVAAVTA
                     VMSLILWGLDGILVRLVSFITGLRF"
     gene            846..1391
                     /gene="nusG"
                     /locus_tag="ACHKBL_001250"
     CDS             846..1391
                     /gene="nusG"
                     /locus_tag="ACHKBL_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005280494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HFO5818143.1"
                     /translation="MSEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEELFGEVMV
                     PTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASWHLVRSVPRVMGFIGGTSDRPAP
                     ISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
                     SVSIFGRATPVELDFSQVEKA"
     gene            1550..1978
                     /gene="rplK"
                     /locus_tag="ACHKBL_001251"
     CDS             1550..1978
                     /gene="rplK"
                     /locus_tag="ACHKBL_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HFO5818144.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     KTDSIEKGLPIPVVITVYADRSFTFVTKTPPAAVLLKKAAGIKSGSGKPNKDKVGKIS
                     RAQLQEIAQTKAADMTGADIEAMTRSIEGTARSMGLVVED"
     gene            1982..2686
                     /gene="rplA"
                     /locus_tag="ACHKBL_001252"
     CDS             1982..2686
                     /gene="rplA"
                     /locus_tag="ACHKBL_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HFO5818145.1"
                     /translation="MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESVDV
                     AVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLAD
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTM
                     GAGVAVDQAGLSASVN"
     gene            3099..3596
                     /gene="rplJ"
                     /locus_tag="ACHKBL_001253"
     CDS             3099..3596
                     /gene="rplJ"
                     /locus_tag="ACHKBL_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HFO5818146.1"
                     /translation="MALNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKA
                     GREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIAYSMEHPGAAARLFKEFAKA
                     NAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKEASAGKLVRTLAAVRD
                     AKEAA"
     gene            3663..4028
                     /gene="rplL"
                     /locus_tag="ACHKBL_001254"
     CDS             3663..4028
                     /gene="rplL"
                     /locus_tag="ACHKBL_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HFO5818147.1"
                     /translation="MSITKDQIIEAVAAMSVMDVVELISAMEEKFGVSAAAAVAVAAG
                     PVEAAEEKTEFDVILKAAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGVSKD
                     DAEALKKALEEAGAEVEVK"
     gene            4348..8376
                     /gene="rpoB"
                     /locus_tag="ACHKBL_001255"
     CDS             4348..8376
                     /gene="rpoB"
                     /locus_tag="ACHKBL_001255"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004870488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFO5818148.1"
                     /translation="MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPE
                     GQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRL
                     VIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTE
                     QILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQ
                     LEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIE
                     TLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSE
                     DRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGN
                     RRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEG
                     PNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLD
                     EEGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDE
                     MYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERGDVLADGPSTDLGELALGQ
                     NMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPN
                     VGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLF
                     SRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLE
                     AKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYD
                     ENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQ
                     RAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDL
                     PTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGK
                     AQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
                     ESFNVLLKEIRSLGINIELEDE"
     gene            8453..12676
                     /gene="rpoC"
                     /locus_tag="ACHKBL_001256"
     CDS             8453..12676
                     /gene="rpoC"
                     /locus_tag="ACHKBL_001256"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HFO5818149.1"
                     /translation="MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQIL
                     TEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLT
                     KRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLAT
                     TIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVL
                     GLYYMTRDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDANGELVAK
                     TSLKDTTVGRAILWMIVPKGLPYSIVNQALGKKAISKMLNTCYRILGLKPTVIFADQI
                     MYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKV
                     IDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADI
                     LVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARGHIINKGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKLSNVKSVVNSSGK
                     LVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITE
                     VSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDV
                     LIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLFE
                     ARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERV
                     ERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLR
                     KATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEA
                     PAAPQVTAEDASASLAELLNAGLGGSDNE"
     gene            complement(12916..14049)
                     /gene="thiH"
                     /locus_tag="ACHKBL_001257"
     CDS             complement(12916..14049)
                     /gene="thiH"
                     /locus_tag="ACHKBL_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000847471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HFO5818150.1"
                     /translation="MKTFSDRWRQLDWDDIRLRINSKTAADVERALNASQLTRDDMMA
                     LLSPAASGYLEPLAQRAQRLTRQRFGNVVSFYVPLYLSNLCANDCTYCGFSMSNRIKR
                     KTLDEADIARESAAIREMGFEHLLLVTGEHQVKVGMDYFRRHLPALREQFSSLQMEVQ
                     PLAETEYAELKQLGLDGVMVYQETYHEATYARHHLKGKKQDFFWRLETPDRLGRAGID
                     KIGLGALIGLSDSWRVDCYMVAEHLLWLQQHYWQSRYSISFPRLRPCTGGIEPASIMD
                     ERQLVQTICAFRLLAPEIELSLSTRESPWFRDRVIPLAINNVSAFSKTQPGGYADNHP
                     ELEQFSPHDDRRPEAVAAALTAQGLQPVWKDWDSYLGRASQRP"
     gene            complement(14046..14816)
                     /gene="thiG"
                     /locus_tag="ACHKBL_001258"
     CDS             complement(14046..14816)
                     /gene="thiG"
                     /locus_tag="ACHKBL_001258"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HFO5818151.1"
                     /translation="MLRIADKTFDSHLFTGTGKFASSQLMMEAIRACGSQLVTLAMKR
                     VNLRQHNDAILEPLIAAGVTLLPNTSGAKTAEEAIFAAHLAREALGTNWLKLEIHPDA
                     RWLLPDPIETLKAAETLVQQGFVVLPYCGADPVLCKRLEEVGCAAVMPLGAPIGSNQG
                     LETRAMLEIITQQATVPVVVDAGIGVPSHAAQALEMGADAVLVNTAIAVADDPVNMAK
                     AFRLAVEAGLLARQSGPGSRSHFAHATSPLTGFLEASA"
     gene            complement(14818..15018)
                     /gene="thiS"
                     /locus_tag="ACHKBL_001259"
     CDS             complement(14818..15018)
                     /gene="thiS"
                     /locus_tag="ACHKBL_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HFO5818152.1"
                     /translation="MQILFNDQPMQCAAGQTVHELLEQLDQRQSGAALAINQQIVPRE
                     QWAQHIVQDGDQILLFQVIAGG"
     gene            complement(15002..15757)
                     /gene="thiF"
                     /locus_tag="ACHKBL_001260"
     CDS             complement(15002..15757)
                     /gene="thiF"
                     /locus_tag="ACHKBL_001260"
                     /EC_number="2.7.7.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole biosynthesis adenylyltransferase ThiF"
                     /protein_id="HFO5818153.1"
                     /translation="MNDRDFMRYSRQILLDDIALDGQQKLLDSQVLIIGLGGLGTPAA
                     LYLAGAGVGTLVLADDDDVHLSNLQRQILFTTEDIDRPKSQVSHQRLTQLNPDIQLTA
                     LQQRLTGEALKDAVARADVVLDCTDNMATRQEINAACVALNTPLITASAVGFGGQLMV
                     LTPPWEQGCYRCLWPDNQEPERNCRTAGVVGPVVGVMGTLQALEAIKLLSGIETPAGE
                     LRLFDGKSSQWRNLALRRASGCPVCGGSNADPV"
     gene            complement(15750..16385)
                     /gene="thiE"
                     /locus_tag="ACHKBL_001261"
     CDS             complement(15750..16385)
                     /gene="thiE"
                     /locus_tag="ACHKBL_001261"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HFO5818154.1"
                     /translation="MYQPDFPPVPFRLGLYPVVDSVQWIERLLDAGVRTLQLRIKDRR
                     DEEVEADVVAAIALGRRYNARLFINDYWRLAIKHQAYGVHLGQEDLQATDLSAIRAAG
                     LRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLADYP
                     TVAIGGISLARAPAVIATGVGSIAVVSAITQAADWRLATAQLLEIAGVGDE"
     gene            complement(16385..18280)
                     /gene="thiC"
                     /locus_tag="ACHKBL_001262"
     CDS             complement(16385..18280)
                     /gene="thiC"
                     /locus_tag="ACHKBL_001262"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HFO5818155.1"
                     /translation="MSVTKLTRREQRAQAQHFIDTLEGTAFPNSKRIYITGTHPGVRV
                     PMREIQLSPTLIGGSKEQPQYEENEAIPVYDTSGPYGDPQIAINVQQGLAKLRQPWID
                     ARGDTEELTVRSSDYTKARLADDGLDELRFSGVLTPKRAKAGRRVTQLHYARQGIITP
                     EMEFIAIRENMGREHIRSEVLRHQHPGMSFGARLPENITAEFVRDEVAAGRAIIPANI
                     NHPESEPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYI
                     HETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEAFRDTLLEQAEQGVDYFTIHA
                     GVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSL
                     GDGLRPGSIQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEE
                     LEHCHEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKE
                     DVKQGLITYKIAAHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFTARAYH
                     DETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAAAQTIEVGMADMSENFRARGGEI
                     YLRKEEA"
     regulatory      complement(18361..18461)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(18513..18989)
                     /gene="rsd"
                     /locus_tag="ACHKBL_001263"
     CDS             complement(18513..18989)
                     /gene="rsd"
                     /locus_tag="ACHKBL_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma D regulator"
                     /protein_id="HFO5818156.1"
                     /translation="MLNQLDNLTERVRGSNKLVDRWLHVRKHLLVAYYNLVGIKPGKE
                     SYMRLNEKALDDFCQSLVDYLSAGHFSIYERILHKLEGNGQLARAAKIWPQLEANTQQ
                     IMDYYDSSLETAIDHDNYLEFQQVLSDIGEALEARFVLEDKLILLVLDAARVKHPA"
     gene            19084..19857
                     /gene="nudC"
                     /locus_tag="ACHKBL_001264"
     CDS             19084..19857
                     /gene="nudC"
                     /locus_tag="ACHKBL_001264"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HFO5818157.1"
                     /translation="MDRIIEKLDHGWWVVSHEQKLWLPKGELPYGEAANFDLVGQRAL
                     QIGEWQGEPVWLVQQQRRHDMGSVRQVIDLDVGLFQLAGRGVQLAEFYRSHKYCGYCG
                     HEMYPSKTEWAMLCSHCRERYYPQIAPCIIVAIRRDDSILLAQHTRHRNGVHTVLAGF
                     VEVGETLEQAVAREVMEESGIKVKNLRYVTSQPWPFPQSLMTAFMAEYDSGDIVIDPK
                     ELLEANWYRYDDLPLLPPPGTVARRLIEDTVAMCRAEYE"
     gene            19897..20961
                     /gene="hemE"
                     /locus_tag="ACHKBL_001265"
     CDS             19897..20961
                     /gene="hemE"
                     /locus_tag="ACHKBL_001265"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HFO5818158.1"
                     /translation="MTELKNDRYLRALLRQPVDVTPVWMMRQAGRYLPEYKATRAQAG
                     DFMSLCKNAELACEVTLQPLRRYPLDAAILFSDILTVPDAMGLGLYFEAGEGPRFTSP
                     VTCKADVDKLPIPDPEDELGYVMNAVRTIRRELKGEVPLIGFSGSPWTLATYMVEGGS
                     SKAFTVIKKMMYADPQALHALLDKLAKSVTLYLNAQIKAGAQAVMIFDTWGGVLTGRD
                     YQQFSLYYMHKIVDGLLRENDGRRVPVTLFTKGGGQWLEAMAETGCDALGLDWTTDIA
                     DARRRVGNKVALQGNMDPSMLYAPPARIEEEVATILAGFGHGEGHVFNLGHGIHQDVP
                     PEHAGVFVEAVHRLSAPYHL"
     gene            20971..21642
                     /gene="nfi"
                     /locus_tag="ACHKBL_001266"
     CDS             20971..21642
                     /gene="nfi"
                     /locus_tag="ACHKBL_001266"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312948.1"
                     /note="cleaves DNA at apurinic or apyrimidinic sites;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HFO5818159.1"
                     /translation="MDLASLRAQQIELASSVIREDRLDKDPPDLIAGADVGFEQGGEV
                     TRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSFREYPALLAAWEMLSQKPDLVFV
                     DGHGISHPRRLGVASHFGLLVDVPTIGVAKKRLCGKFEPLSSEPGALAPLMDKGEQLA
                     WVWRSKARCNPLFIATGHRVSVDSALAWVQRCMKGYRLPEPTRWADAVASERPAFVRY
                     TANQP"
     gene            21685..22275
                     /gene="yjaG"
                     /locus_tag="ACHKBL_001267"
     CDS             21685..22275
                     /gene="yjaG"
                     /locus_tag="ACHKBL_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaG family protein"
                     /protein_id="HFO5818160.1"
                     /translation="MLQNPIHLRLERLESWQHVTFMACLCERMYPNYAVFCQQTGFGD
                     GQIYRRILDLIWETLTVKDAKVNFDSQLEKFEEAIPSADDFDLYGVYPAIDACVALSE
                     LVHSRLSGETLEHAVEVSKTSITTVAMLEMTQAGREMSDEELKENPAVEQEWDIQWEI
                     FRLLAECEERDIELIKGLRADLREAGESNIGIIFQQ"
     gene            22462..22734
                     /gene="hupA"
                     /locus_tag="ACHKBL_001268"
     CDS             22462..22734
                     /gene="hupA"
                     /locus_tag="ACHKBL_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein HU-alpha"
                     /protein_id="HFO5818161.1"
                     /translation="MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQL
                     VGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK"
     gene            22747..23442
                     /gene="yjaH"
                     /locus_tag="ACHKBL_001269"
     CDS             22747..23442
                     /gene="yjaH"
                     /locus_tag="ACHKBL_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1481 domain-containing protein"
                     /protein_id="HFO5818162.1"
                     /translation="MNSFNEGVVSPLLSFWRRSLMLAGALFLTACSHNSSLPPFTASG
                     FAEDQGAVRIWRKDSGDNVHLLAVFSPWRSGDTTTREYRWQGDNLTLININVYSKPPV
                     NIRARFDDRGDLSFMQRESDGEKQQLSNDQIDLYRYRADQIRQISDALRQGRVVLRQG
                     RWHAMEQTVTTCEGQTIKPDLDSQAIAHIERRQSRSSVDVSVAWLEAPEGSQLLLVAN
                     SDFCRWQPNEKTF"
     gene            complement(23444..23863)
                     /gene="zraP"
                     /locus_tag="ACHKBL_001270"
     CDS             complement(23444..23863)
                     /gene="zraP"
                     /locus_tag="ACHKBL_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc resistance sensor/chaperone ZraP"
                     /protein_id="HFO5818163.1"
                     /translation="MKRNTKIALVMMALSAMAMGSTSAFAHGGHGMWQQNAAPLTSEQ
                     QTAWQKIHNDFYAQSSALQQQLVTKRYEYNALLAANPPDSSKINAVAKEMENLRQSLD
                     ELRVKRDIAMAEAGIPRGTGMGYGGCGGGGHMGMDHW"
     gene            24101..25477
                     /gene="zraS"
                     /locus_tag="ACHKBL_001271"
     CDS             24101..25477
                     /gene="zraS"
                     /locus_tag="ACHKBL_001271"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     ZraS"
                     /protein_id="HFO5818164.1"
                     /translation="MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAA
                     ARQTLLEKGSVLIRALESGSRVGMGMRMHHAQQQALLEEMAGQPGVRWFAVTDEQGTI
                     VMHSNSGMVGKQLYSPQEMQQLHPGDEEAWRRIDSADGEPVLEIYRQFQPMFAAGMHR
                     MRHMQQYAATPQAIFIAFDASNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRY
                     LRSGQLLQDEMKRKEKLVALGHLAAGVAHEIRNPLSSIKGLAKYFAERAPAGGEAHQL
                     AQVMAKEADRLNRVVSELLELVKPTHLALQAVDLNTLINHSLQLVSQDANSREIQLRF
                     TANDTLPEIQADPDRLTQVLLNLYLNAIQAIGQHGVISVTASESGTGVKISVTDSGKG
                     IAADQLEAIFTPYFTTKAEGTGLGLAVVHNIVEQHGGTIQVASQVGKGATFTLWLPVT
                     ITRKDPQG"
     gene            25474..26799
                     /gene="zraR"
                     /locus_tag="ACHKBL_001272"
     CDS             25474..26799
                     /gene="zraR"
                     /locus_tag="ACHKBL_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor ZraR"
                     /protein_id="HFO5818165.1"
                     /translation="MTHDNIDILVVDDDISHCTILQALLRGWGYNVALANSGRQALEQ
                     VREQVFDLVLCDVRMAEMDGIATLKEIKALNPAIPVLIMTAYSSVETAVEALKTGALD
                     YLIKPLDFDNLQATLEKALAHTHSVDAETPAVSASQFGMVGKSPAMQHLLSEIALVAP
                     SEATVLIHGDSGTGKELVARAIHASSARSEKPLVTLNCAALNESLLESELFGHEKGAF
                     TGADKRREGRFVEADGGTLFLDEIGDISPMMQVRLLRAIQEREVQRVGSNQTISVDVR
                     LIAATHRDLAAEVNAGRFRQDLYYRLNVVAIEVPSLRQRREDIPLLANHFLQRFAERN
                     RKAVKGFTPQAMDLLIHYDWPGNIRELENTVERAVVLLTGEYISERELPLAIASTPIP
                     LVQSQDIQPLVEVEKEVILAALEKTGGNKTEAARQLGITRKTLLAKLSR"
     gene            complement(26796..28085)
                     /gene="purD"
                     /locus_tag="ACHKBL_001273"
     CDS             complement(26796..28085)
                     /gene="purD"
                     /locus_tag="ACHKBL_001273"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HFO5818166.1"
                     /translation="MKVLVIGNGGREHALAWKAAQSPLVETVFVAPGNAGTALEPTLQ
                     NVAIGVTDIPALLDFAQNEKIDLTIVGPEAPLVKGVVDTFRAAGLKIFGPTAGAAQLE
                     GSKAFTKDFLARHNIPTAEYQNFTEVEPALAYLREKGAPIVIKADGLAAGKGVIVAMT
                     LEEAEAAVHDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRV
                     GDKDTGPNTGGMGAYSPAPVVTDEVHQRTMERIIWPTVKGMAAEGNTYTGFLYAGLMI
                     DKQGNPKVIEFNCRFGDPETQPIMLRMKSDLVELCLAACEGKLDEKTSEWDERASLGV
                     VMAAGGYPGDYRTGDVIHGLPLEEVADGKVFHAGTKLADDEQVVTSGGRVLCVTALGH
                     TVAEAQKRAYALMTDIHWDDCFCRKDIGWRAIEREQN"
     gene            complement(28097..29686)
                     /gene="purH"
                     /locus_tag="ACHKBL_001274"
     CDS             complement(28097..29686)
                     /gene="purH"
                     /locus_tag="ACHKBL_001274"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HFO5818167.1"
                     /translation="MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLL
                     AEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGQDDAIMEEHQIQPIDMVV
                     VNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEM
                     DANEGSLLLATRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNL
                     NFIKKQDMRYGENSHQQAAFYIEENVKEASVATATQVQGKALSYNNIADTDAALECVK
                     EFAEPACVIVKHANPCGVAISNSILDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAI
                     ISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERVPGLDFKRVNGGLLVQDRD
                     LGMVGAEELRVVTKRQPTEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSR
                     VYSAKIAGIKAADEGLEVKGSSMASDAFFPFRDGIDAAAAAGVTCVIQPGGSIRDDEV
                     IAAADEHGIAMLFTDMRHFRH"
BASE COUNT         7055 a         7829 c         8318 g         6954 t
ORIGIN      
        1 cgtcatactc cgttcttcaa aggctaccgt ccgcagttct acttccgtac tactgacgtg
       61 actggtacca tcgaactgcc ggaaggtgta gagatggtaa tgccgggcga caacatcaaa
      121 atggttgtta ccctgatcca cccgatcgcg atggacgacg gtctgcgttt cgcaatccgt
      181 gaaggcggcc gtaccgttgg cgcgggcgtt gtagcaaaag ttctgagcta attgccgata
      241 acatttgacg caatgcgcac taaaagggca tcatttgatg ccctttttgc acgctttcgt
      301 accagaacct ggctcatcag tgattttctt tgtcataatc attgctgaga caggctctgt
      361 tgagggcgta taatccgaaa agctaatacg cgtttcgatt tggtttgcct cgcgatcgcg
      421 gggtgaaaat gtttgtagaa tacttctgac aggttggttt atgagtgcga ataccgaagc
      481 tcaaggaagc gggcgcggcc tggaagcgat gaagtgggtc gttgtggtgg cattgctcct
      541 ggtagcgatt gtcggcaact acctttatcg cgacattatg ctgccgctgc gtgcgctggc
      601 cgtagtaatt ctgattgctg cagcgggtgg tgtcgcgctg ttaacgacaa aaggtaaagc
      661 aaccgttgct tttgcccgtg aagcgcgtac cgaagtccgt aaggtcattt ggccgactcg
      721 ccaggaaaca ttgcacacca cgctgattgt ggctgcggtt accgcagtaa tgtcactgat
      781 cctgtgggga ctggatggta ttctggttcg cctggtatcc tttatcactg gcctgaggtt
      841 ctgagatgtc tgaagctcct aaaaagcgct ggtacgtcgt tcaggcgttt tccggttttg
      901 aaggccgcgt agcaacgtcg ctgcgtgagc atatcaaatt acacaacatg gaagagttgt
      961 ttggtgaagt catggtacca accgaagaag tggttgaaat ccgtggcggt cagcgtcgca
     1021 aaagcgaacg taaattcttc cctggctacg tcctcgttca gatggtgatg aacgacgcga
     1081 gctggcacct ggtgcgcagc gtaccgcgtg tgatgggctt catcggcggt acttccgatc
     1141 gtcctgcgcc aatcagcgat aaagaagtcg atgcgattat gaaccgcctg cagcaggttg
     1201 gtgataagcc gcgtccgaaa acgttgtttg aaccgggtga aatggtccgt gttaatgatg
     1261 gtccgttcgc tgacttcaac ggcgttgttg aagaagttga ttacgagaaa tctcgtctga
     1321 aagtgtctgt ttctatcttc ggtcgtgcga cccctgtaga gctggacttc agccaggttg
     1381 aaaaagctta acccagcgat caaaaaagcg gcgatttaat cgttgcacaa ggcgcgagat
     1441 tggaatacaa tttcgcgcct tttgttttta tgggccttgc ccgtaaaacg attttttata
     1501 tcacggggag cctctcagag gcgttattac ccaacttgag gaatttataa tggctaagaa
     1561 agtacaagcc tatgtcaagc tgcaggttgc agctggtatg gctaacccga gtccgccagt
     1621 aggtccggct ctgggtcagc agggcgtaaa catcatggaa ttctgcaaag cgttcaacgc
     1681 aaaaactgat tccatcgaaa aaggtctgcc gattccggta gtaatcactg tttacgctga
     1741 ccgttctttc actttcgtta ccaagactcc gccggcagca gttctgctga aaaaagcggc
     1801 tggtatcaag tctggttccg gtaagccgaa caaagacaaa gtgggtaaaa tttcccgcgc
     1861 tcagctgcag gaaatcgcgc agaccaaagc tgccgacatg actggtgccg acattgaagc
     1921 gatgactcgc tccatcgaag gtactgcacg ttccatgggc ctggtagtgg aggactaaga
     1981 aatggctaaa ctgaccaagc gcatgcgtgt tatccgagag aaagttgatg caaccaaaca
     2041 gtacgacatc aacgaagcta tcgctctgct gaaagaactg gcgactgcta aattcgtaga
     2101 aagcgtggac gtagctgtta acctcggcat cgacgctcgt aaatctgacc agaacgtacg
     2161 tggtgcaact gtactgccgc acggtactgg ccgttccgtt cgcgtagccg tatttaccca
     2221 gggtgcaaac gctgaagctg ctaaagctgc aggtgctgaa ctggtaggta tggaagatct
     2281 ggctgaccag atcaagaaag gcgaaatgaa ctttgacgtt gttattgctt ctccggatgc
     2341 aatgcgcgtt gttggccagc tgggccaggt tctgggtccg cgcggcctga tgccaaaccc
     2401 gaaagtgggt actgtaacgc cgaacgttgc tgaagcggtt aaaaacgcta aagctggcca
     2461 ggttcgttac cgtaacgaca aaaacggcat catccacacc accatcggta aagtggactt
     2521 tgacgctgac aaactgaaag aaaacctgga agctctgctg gttgcgctga aaaaagcaaa
     2581 accgactcag gcgaaaggcg tgtacatcaa gaaagttagc atctccacca ccatgggtgc
     2641 aggtgttgca gttgaccagg ctggcctgag cgcttctgta aactaatgcc tttacgtggg
     2701 cggtgatttt gtctacaatc ttacccccac gtataatgct taatgcagac gtatatccga
     2761 gatattcggg ttgtggcaag gcggtaactg agtgagtcgc caggagcata gttaactatg
     2821 tgactggtgc gaacgaagga agccaacgcc gccacaagct gaatagcgac ggatagaaaa
     2881 gatttgttcg ttggagcctg gcctatccag gcctccgtcg aagaccgcag gagtttcgca
     2941 agaaacttaa tcccctgcgt agacggtgac agaacgctaa gattattctt ttatattctg
     3001 gcttgtttct gctcaccgta attaagacgc tctctccgtt tggaggagtg aagtgagttc
     3061 cagagatttt ctctggcaaa catccaggag caaagctaat ggctttaaat cttcaagaca
     3121 aacaagcgat tgttgctgaa gtcagcgaag tagccaaagg cgcgctgtct gcagtagttg
     3181 cggattcccg tggcgtaact gtagataaaa tgactgaact gcgtaaagca ggtcgcgaag
     3241 ctggcgtata catgcgtgtt gttcgtaaca ccctgctgcg ccgtgctgtt gaaggtactc
     3301 cgttcgagtg cctgaaagac gcgtttgttg gtccgaccct gattgcatac tctatggaac
     3361 acccgggcgc tgctgctcgt ctgttcaaag agttcgcgaa agcgaatgca aaatttgagg
     3421 tcaaagccgc tgcctttgaa ggtgagctga tcccggcgtc tcagatcgac cgcctggcaa
     3481 ctctgccgac ctacgaagaa gcaattgcac gcctgatggc aaccatgaaa gaagcttcgg
     3541 ctggcaaact ggttcgtact ctggctgctg tacgcgatgc gaaagaagct gcttaatcgc
     3601 agttatcttt ttaacgcatt cgcttacgta taaacttatt ctgatattca ggaacaattt
     3661 aaatgtctat cactaaagat caaatcattg aagcagttgc agctatgtct gtaatggacg
     3721 ttgtagaact gatctctgca atggaagaaa aattcggtgt ttccgctgct gctgctgtag
     3781 ctgtagctgc tggcccggtt gaagctgctg aagaaaaaac tgaattcgac gtaattctga
     3841 aagctgctgg cgctaacaaa gttgctgtta tcaaagcagt acgtggcgca actggcctgg
     3901 gtctgaaaga agctaaagac ctggtagaat ctgcaccggc tgctctgaaa gaaggcgtga
     3961 gcaaagacga cgcagaagca ctgaaaaaag ctctggaaga agctggcgct gaagttgaag
     4021 ttaaataagc caacccttcc ggttgcagcc tgagaaatca ggctgatggc tggtgacttt
     4081 ttagtcacca gcctttttgc gctgtaaggc gccagtagcg tttcacactg tttgactact
     4141 gctgtgcctt tcaatgcttg tttctatcga cgacttaata tactgcgaca ggacgtccgt
     4201 tctgtgtaaa tcgcaatgaa atggtttaag cgtgatagca acaggcattg cggaaagtgt
     4261 tccattttcc ggtcaacaaa atagtgttgc acaaactgtc cgctcaatgg acagatgggt
     4321 cgacttgtca gcgagctgag gaaccctatg gtttactcct ataccgagaa aaaacgtatt
     4381 cgtaaggatt ttggtaaacg tccacaagtt ctggatgtac cttatctcct ttctatccag
     4441 cttgactcgt ttcagaaatt tatcgagcaa gatcctgaag ggcagtatgg tctggaagct
     4501 gctttccgtt ccgtattccc gattcagagc tacagcggta attccgagct gcaatacgtc
     4561 agctaccgcc ttggcgaacc ggtgtttgac gtccaggaat gtcaaatccg tggcgtgacc
     4621 tattccgcac cgctgcgcgt taaactgcgt ctggtgatct atgagcgcga agcgccggaa
     4681 ggcaccgtaa aagacattaa agaacaagaa gtctacatgg gcgaaattcc gctcatgaca
     4741 gacaacggta cctttgttat caacggtact gagcgtgtta tcgtttccca gctgcaccgt
     4801 agtccgggcg tcttctttga ctccgacaaa ggtaaaaccc actcttcggg taaagtgctg
     4861 tataacgcgc gcatcatccc ttaccgtggt tcctggcttg acttcgaatt cgatccgaag
     4921 gacaacctgt tcgtacgtat cgaccgtcgc cgtaaactgc ctgcgaccat cattctgcgc
     4981 gccctgaact acaccacaga gcagatcctc gacctgttct ttgaaaaagt tatctttgaa
     5041 atccgtgata acaagctgca gatggaactg gtgccggaac gcctgcgtgg tgaaaccgca
     5101 tcttttgaca tcgaagctaa cggtaaagtg tacgtagaaa aaggccgccg tatcactgcg
     5161 cgccacattc gccagctgga aaaagacgac gtcaaactga tcgaagtccc ggttgagtac
     5221 atcgcaggta aagtggttgc taaagactat attgatgagt ctaccggcga gctgatctgt
     5281 gctgcgaaca tggagctgag cctggatctg ctggctaagc tgagccagtc tggtcacaag
     5341 cgtatcgaaa cgctgttcac caacgatctg gatcacggcc catatatctc tgaaacctta
     5401 cgtgtcgacc caactaacga ccgtctgagc gcactggtag aaatctaccg catgatgcgc
     5461 cctggcgagc cgccgactcg tgaagcagct gaaagcctgt tcgagaacct gttcttctcc
     5521 gaagaccgtt atgacttgtc tgcggttggt cgtatgaagt tcaaccgttc tctgctgcgc
     5581 gaagaaatcg aaggttccgg tattctgagc aaagacgaca tcattgatgt tatgaaaaag
     5641 ctcatcgata tccgtaacgg taaaggcgaa gtcgatgata tcgaccacct cggcaaccgt
     5701 cgtatccgtt ccgttggcga aatggcggaa aaccagttcc gcgttggcct ggtacgtgta
     5761 gagcgtgcgg tgaaagagcg tctgtctctg ggcgatctgg ataccctgat gcctcaggat
     5821 atgatcaacg ccaagccgat ttccgcagca gtgaaagagt tcttcggttc cagccagctg
     5881 tctcagttta tggaccagaa caacccgctg tctgagatta cgcacaaacg tcgtatctcc
     5941 gcactcggcc caggcggtct gacccgtgaa cgtgcaggct tcgaagttcg agacgtacac
     6001 ccgactcact acggtcgcgt atgtccaata gaaacccctg aaggtccgaa catcggtctg
     6061 atcaactctc tgtccgtgta cgcacagact aacgaatacg gcttccttga gactccgtat
     6121 cgtaaagtga ccgacggtgt tgtaactgac gaaattcact acctatctgc tatcgaagaa
     6181 ggcaactacg ttatcgccca ggcgaactcc aacctggatg aagaaggcca cttcgtagaa
     6241 gacctggtaa cctgccgtag caaaggcgaa tccagcttgt tcagccgcga ccaggttgac
     6301 tacatggacg tatccaccca gcaggtggta tccgtcggtg cgtccctgat cccgttcctg
     6361 gaacacgatg acgccaaccg tgcattgatg ggtgcgaaca tgcaacgtca ggccgttccg
     6421 actctgcgcg ctgacaagcc gctggttggt actggtatgg aacgtgctgt tgccgttgac
     6481 tccggtgtaa ctgcggtagc taaacgtggt ggtgtcgttc agtacgtgga tgcttcccgt
     6541 atcgttatca aagttaacga agacgagatg tatccgggtg aagcaggtat cgatatctac
     6601 aacctgacca aatacacccg ttctaaccag aacacctgta tcaaccagat gccgtgtgtg
     6661 tctctgggtg aaccggttga acgtggcgac gtgctggcag acggtccgtc caccgacctc
     6721 ggtgaactgg cgcttggtca gaacatgcgc gtagcgttca tgccgtggaa tggttacaac
     6781 ttcgaagact ccatcctcgt atccgagcgt gttgttcagg aagaccgttt caccaccatc
     6841 cacattcagg aactggcgtg tgtgtcccgt gacaccaagc tggggccaga agagatcacc
     6901 gctgacatcc cgaacgtggg tgaagctgcg ctctccaaac tggatgaatc cggtatcgtt
     6961 tacattggtg cggaagtgac cggtggcgac attctggttg gtaaggttac gccgaaaggt
     7021 gaaactcagc tgaccccaga agaaaaactg ctgcgtgcga tcttcggtga gaaagcgtct
     7081 gacgttaaag actcttctct gcgcgtacca aacggtgtat ccggtacggt tatcgacgtt
     7141 caggtcttta ctcgcgatgg cgtagaaaaa gacaaacgtg cgctggaaat cgaagaaatg
     7201 cagctcaaac aggcgaagaa agacctgtct gaagaactgc agatcctcga agcgggtctg
     7261 ttcagccgta tccgtgctgt gctggtagcc ggtggcgttg aagctgagaa gctcgacaaa
     7321 ctgccgcgcg atcgctggct ggagctgggc ctgaccgacg aagagaaaca aaatcagctg
     7381 gaacagctgg ctgagcagta tgacgaactg aaacacgagt tcgagaagaa actcgaagcg
     7441 aaacgccgca aaatcaccca gggcgacgat ctggcaccgg gcgtgctgaa gattgttaag
     7501 gtatatctgg cggttaaacg ccgtatccag cctggtgaca agatggcagg tcgtcatggt
     7561 aacaagggtg taatttctaa gatcaacccg atcgaagata tgccttacga tgaaaacggt
     7621 acgccggtag acatcgtact gaacccgctg ggcgtaccgt ctcgtatgaa catcggtcag
     7681 atcctcgaaa cccacctggg tatggctgcg aaaggtatcg gcgacaagat caacgccatg
     7741 ctgaaacagc agcaagaagt cgcgaaactg cgcgaattca tccagcgtgc gtacgatctg
     7801 ggcgctgacg ttcgtcagaa agttgacctg agtaccttca gcgatgaaga agttatgcgt
     7861 ctggctgaaa acctgcgcaa aggtatgcca atcgcaacgc cggtgttcga cggtgcgaaa
     7921 gaagcagaaa ttaaagagct gctgaaactt ggcgacctgc cgacttctgg tcagatccgc
     7981 ctgtacgacg gccgcactgg tgaacagttc gagcgtccgg taaccgttgg ttacatgtac
     8041 atgctgaaac tgaaccacct ggtcgacgac aagatgcacg cgcgttccac cggttcttac
     8101 agcctggtta ctcagcagcc gctgggtggt aaggcacagt tcggtggtca gcgtttcggg
     8161 gagatggaag tgtgggcgct ggaagcatac ggcgcagcat acaccctgca ggaaatgctc
     8221 accgttaagt ctgatgacgt gaacggtcgt actaagatgt ataaaaacat cgtggacggc
     8281 aaccatcaga tggagccggg catgccagaa tccttcaacg tattgttgaa agagattcgt
     8341 tcgctgggta tcaacatcga actggaagac gagtaattct cgctcaaaca ggtcactgct
     8401 gtcggggtaa aacccggcag cggattgtgc taactccgac gggagcaaat ccgtgaaaga
     8461 tttattaaag tttctgaaag cgcagactaa aaccgaagag tttgatgcga tcaaaattgc
     8521 tctggcttcg ccagacatga tccgttcatg gtctttcggt gaagttaaaa agccggaaac
     8581 catcaactac cgtacgttca aaccagaacg tgacggcctt ttctgcgccc gtatctttgg
     8641 gccggtaaaa gattacgagt gcctgtgcgg taagtacaag cgcctgaaac accgtggtgt
     8701 catctgtgag aagtgcggcg ttgaagtgac ccagactaaa gtacgccgtg agcgtatggg
     8761 ccacatcgaa ctggcttccc cgactgcgca catctggttc ctgaaatcgc tgccgtcccg
     8821 tatcggcctg ctgctcgata tgccgctgcg cgatatcgaa cgcgtactgt acttcgaatc
     8881 ctatgtggtt attgaaggcg gtatgaccaa cctggaacgc cagcagatcc tgactgaaga
     8941 gcagtatctg gacgcgctgg aagagttcgg tgacgaattc gacgcgaaaa tgggtgcgga
     9001 agcaatccag gccctgctga agagcatgga tctggagcaa gagtgcgaac agctgcgtga
     9061 agagctgaac gaaaccaact ccgaaaccaa gcgtaaaaag ctgaccaagc gtatcaaact
     9121 gctggaagcg ttcgttcagt ctggtaacaa accagagtgg atgatcctga ccgttctgcc
     9181 ggtactgccg ccagatctgc gtccgctggt tccgctggat ggtggtcgtt tcgcgacttc
     9241 tgacctgaac gatctgtatc gtcgcgtcat taaccgtaac aaccgtctga aacgtctgct
     9301 ggatctggct gcgccggaca tcatcgtacg taacgaaaaa cgtatgctgc aggaagcggt
     9361 agacgccctg ctggataacg gtcgtcgcgg tcgtgcgatc accggttcta acaagcgtcc
     9421 tctgaaatct ttggccgaca tgatcaaagg taaacagggt cgtttccgtc agaacctgct
     9481 cggtaagcgt gttgactact ccggtcgttc tgtaatcacc gtaggtccat acctgcgtct
     9541 gcatcagtgc ggtctgccga agaaaatggc gctggagctg ttcaaaccgt tcatctacgg
     9601 caagctggaa ctgcgtggtc ttgctaccac cattaaagct gcgaagaaaa tggttgagcg
     9661 cgaagaagct gtcgtttggg atatcctgga cgaagttatc cgcgaacacc cggtactgct
     9721 gaaccgtgca ccgactctgc accgtctggg tatccaggca tttgaaccgg tactgatcga
     9781 aggtaaagct atccagctgc acccgctggt ttgtgcggca tataacgccg acttcgatgg
     9841 tgaccagatg gcagttcacg taccgctgac gctggaagcc cagctggaag cgcgtgcgct
     9901 gatgatgtct accaacaaca tcctgtcccc ggcgaacggc gaaccaatca tcgttccgtc
     9961 tcaggacgtt gtactgggtc tgtactacat gacccgtgac tgtgttaacg ccaaaggcga
    10021 aggcatggtg ctgactggcc cgaaagaagc agaacgtctg tatcgctctg gtctggcctc
    10081 tctgcatgcg cgcgttaaag tgcgtatcac cgagtatgaa aaagatgcta acggtgaatt
    10141 agtagcgaaa accagcctga aagacacgac tgttggccgt gccattctgt ggatgattgt
    10201 accgaaaggt ctgccttact ccatcgtcaa ccaggcgctg ggtaaaaaag caatctccaa
    10261 aatgctgaac acctgctacc gcattctcgg tctgaaaccg accgttattt ttgcggacca
    10321 gatcatgtac accggctttg catatgcagc gcgttctggt gcatctgttg gtatcgatga
    10381 catggtcatc ccggagaaga aacacgaaat catctccgag gcagaagcag aagttgctga
    10441 aattcaggag cagttccagt ctggtctggt aactgcgggc gaacgctaca acaaagttat
    10501 cgatatctgg gctgcggcga acgatcgtgt atccaaagcg atgatggata acctgcaaac
    10561 tgaaaccgtt attaaccgtg acggtcagga agagaagcag gtttccttca acagcatcta
    10621 catgatggcc gactccggtg cgcgtggttc tgcggcacag attcgtcagc ttgctggtat
    10681 gcgtggtctg atggcgaagc cggatggctc catcatcgaa acgccaatca ccgcgaactt
    10741 ccgtgaaggt ctgaacgtac tccagtactt catctccacc cacggtgctc gtaaaggtct
    10801 ggcggatacc gcactgaaaa cggcgaactc cggttacctg actcgtcgtc tggttgacgt
    10861 ggcgcaggac ctggtggtta ccgaagacga ttgtggtact catgaaggta tcatgatgac
    10921 tccggttatc gaaggtggtg acgttaaaga gccgctgcgc gatcgcgtac tgggtcgtgt
    10981 aactgctgaa gacgttctga agccgggtac tgctgatatc ctcgttccgc gcaacacgct
    11041 gctgcacgaa cagtggtgtg acctgctgga agagaactct gtcgacgcgg ttaaagtacg
    11101 ttctgttgta tcttgtgaca ccgactttgg tgtatgtgcg cactgctacg gtcgtgacct
    11161 ggcgcgtggc cacatcatca acaaaggtga agcaatcggt gttatcgcgg cacagtccat
    11221 cggtgaaccg ggtacacagc tgaccatgcg tacgttccac atcggtggtg cggcatctcg
    11281 tgcggctgct gaatccagca tccaggtgaa aaacaaaggt agcatcaagc tcagcaacgt
    11341 gaagtcggtt gtgaactcca gcggtaaact ggttatcact tcccgtaaca ccgaactgaa
    11401 actgatcgac gaattcggtc gtaccaaaga aagctacaaa gtaccttacg gtgcggtact
    11461 ggcaaaaggc gatggcgaac aggttgctgg cggcgaaacc gttgcaaact gggacccgca
    11521 caccatgccg gttatcaccg aagtaagcgg ttttgtacgc tttactgaca tgatcgacgg
    11581 tcagaccatt acgcgtcaga ccgacgaatt gaccggtctg tcttcgctgg tggttctgga
    11641 ttccgcagaa cgtaccgcag gtggtaaaga tctgcgtccg gcactgaaaa tcgttgatgc
    11701 tcagggtaac gatgtactga tcccgggtac cgatatgcct gcgcagtact tcctgccggg
    11761 taaagcgatt gttcagctgg aagatggcgt acagatcagc tctggtgaca ccctggcgcg
    11821 tattccgcag gaatctggcg gtaccaagga catcaccggt ggtctgccgc gcgttgcgga
    11881 tctgttcgaa gcacgtcgtc cgaaagagcc ggcaatcctg gctgaaatca gcggtatcgt
    11941 ttccttcggt aaagaaacca aaggtaaacg tcgtctggtt atcactccgg tagacggtag
    12001 cgatccgtac gaagagatga ttccaaaatg gcgtcagctc aatgtgttcg aaggtgaacg
    12061 tgtagaacgt ggtgacgtaa tttccgatgg tccggaagcg ccgcacgaca ttctgcgtct
    12121 gcgtggtgtt catgctgtta ctcgttacat cgttaacgaa gtacaagacg tataccgtct
    12181 gcagggcgtt aagattaacg ataaacacat cgaagttatc gttcgtcaga tgctgcgtaa
    12241 agctaccatc gttaacgcgg gcagctccga cttcctggaa ggtgaacagg ttgaatactc
    12301 tcgcgtcaag atcgcaaacc gcgaactgga agcgaacggc aaagtgggtg caacttactc
    12361 ccgcgatctg ctgggtatca ccaaagcgtc tctggcaacc gagtccttca tctccgcggc
    12421 atcgttccag gagaccactc gcgtgctgac cgaagcagcc gttgcgggca aacgcgacga
    12481 actgcgcggc ctgaaagaga acgttatcgt gggtcgtctg atcccggcag gtaccggtta
    12541 cgcgtaccac caggatcgta tgcgtcgccg tgctgcgggt gaagctccgg ctgcaccgca
    12601 ggtgactgca gaagacgcat ctgccagcct ggcagaactg ctgaacgcag gtctgggcgg
    12661 ttctgataac gagtaatcgt taatccgcaa ataacgtaaa aactcgcttc ggcgggtttt
    12721 tttatgggtg ttattcagag ggcaattctt cacggaattt ttaagaaagg cagggcgaaa
    12781 cgaggaagaa gcttttcgcc ctggacaaca ttcctgctga cgggactacc ataaaaaaac
    12841 acaacgcttt caacaacctt gcgtttttca tcttccgtag cggctcggaa taaatttacg
    12901 ttgttacatt ctgtctcatg gcctttgcga ggcgcgtccc agatagctgt cccagtcttt
    12961 ccataccggc tgcaaaccct gagcagttaa cgcggcagca accgcttccg gtctgcgatc
    13021 gtcgtgcggt gagaactgtt ccagctcggg gtgattgtcg gcatagccac ctggctgcgt
    13081 tttcgaaaaa gcgctgacgt tattaattgc cagcggaata acgcgatcgc gaaaccacgg
    13141 tgattcccgc gtggagagtg acagttcaat ctccggtgca agcagccgga aggcgcagat
    13201 ggtttgcact aactggcgtt catccataat cgacgcaggc tcaatgccgc cagtacatgg
    13261 acgcaggcgt gggaaggaga tagagtagcg gctttgccag taatgctgtt gcagccatag
    13321 caaatgttct gcaaccatat agcagtcaac gcgccagctg tcggaaaggc caattagcgc
    13381 gccgaggcct atcttatcaa tccccgcacg gcccagccga tccggcgttt ccagccgcca
    13441 gaagaagtcc tgttttttac ctttcagatg atggcgggca taagtcgcct cgtgatatgt
    13501 ctcctgataa accatcacgc catccagacc tagttgcttt aactcggcgt attccgtctc
    13561 cgccagcggt tgcacttcca tctgtagtga agagaactgt tcacgcaggg cagggagatg
    13621 acgacgaaag taatccatcc ccactttcac ctgatgttca ccagtgacta atagcagatg
    13681 ttcaaagccc atctcccgta tagcggcgct ttccctggca atatccgctt catccagcgt
    13741 tttgcgcttg atgcgattgc tcatggaaaa tccgcagtac gtgcagtcgt tagcgcaaag
    13801 attggaaaga taaagcggga cgtagaagct gacgacgttg ccaaatcgct gacgggtcag
    13861 acgctgcgcc cgttgggcca gtggttccag atagccactg gcggctggcg ataacagcgc
    13921 catcatatcg tcgcgggtga gttgcgaggc atttagcgcc cgctctacgt cagcagccgt
    13981 tttgctgttg atacgcaggc ggatgtcgtc ccagtccagt tgtcgccagc gatcgctgaa
    14041 ggttttcatg ctgatgcctc cagaaatccg gtcagcgggc tggtggcatg agcaaaatga
    14101 ctgcggctgc ccggtccgga ctgacgtgcc agcaggcctg cttctactgc cagacgaaat
    14161 gccttcgcca tgttgacggg atcgtccgcg acggcaatcg ccgtattcac taacaccgcg
    14221 tcggccccca tttccagcgc ctgcgcggca tggctgggaa cgccgatgcc agcatcgaca
    14281 accaccggaa ccgtggcttg ctgggtaata atctccagca tggcacgggt ttccagtccc
    14341 tgattcgagc caatcggcgc gccgagcggc atcaccgctg cacagccgac ttcttccaga
    14401 cgtttacaca ataccggatc ggccccgcag taaggcagca cgacaaatcc ctgttgtacc
    14461 agcgtttcgg cggctttcag ggtttcgatg ggatcgggca acagccagcg ggcgtcaggg
    14521 tgaatctcta attttaacca gtttgtgcct aacgcttcac gagccagatg ggcggcgaaa
    14581 atggcttctt ccgctgtttt cgccccggat gtatttggca gcagggtcac acccgccgcg
    14641 ataagcggtt cgaggatagc gtcgttgtgc tggcgcaagt tgacgcgttt catcgccagt
    14701 gtcaccagtt ggctgccgca agcgcggatc gcctccatca tcagttgtga agaggcgaat
    14761 ttccctgtgc cggtaaacag atgtgaatca aacgttttgt ccgcaatacg taacatctca
    14821 accccctgca ataacctgaa aaagcaggat ctggtcgcca tcctgcacga tatgttgcgc
    14881 ccactgctca cgcgggacga tttgctgatt aatcgccaga gccgcgcccg attgtcgttg
    14941 gtcgagttgc tccagtagtt cgtgaaccgt ttgcccggcg gcgcactgca tcggttgatc
    15001 gttaaacagg atctgcattg cttcctccgc ataccgggca accactggcg cggcgcaacg
    15061 ccaggttgcg ccactggctc gatttaccgt cgaacagtcg gagttctccc gcaggcgtct
    15121 ctataccgct taataactta atggcttcca gtgcctgtaa agtgcccata accccgacca
    15181 ccgggccaac cacgcccgcc gtgcggcagt tgcgttcagg ctcctggtta tctggccaca
    15241 ggcagcggta acacccctgc tcccagggcg gcgtcagtac catcaactga ccgccaaatc
    15301 cgaccgcgct ggcggtgata agcggcgtgt tgagtgccac gcaggcggca ttaatctcct
    15361 gacgagtcgc catattgtcg gtacagtcga gcaccacatc ggcccgtgct accgcatctt
    15421 ttaacgcctc acccgttaac cgttgttgta atgctgtcag ttgaatatcg ggattcaact
    15481 gtgtcagtcg ctgatggctg acctgcgatt tcgggcgatc gatatcttca gtggtaaaga
    15541 ggatttgtcg ttgcagattg cttaaatgca catcgtcgtc atctgccagt accagcgtcc
    15601 cgacgccagc gcccgccagg tatagcgcag caggtgtacc cagcccgcca agaccgataa
    15661 tcagcacctg gctgtcgagc agtttttgct gcccgtcaag agcgatatcg tcgagcagga
    15721 tttggcggct ataacgcata aagtcacggt cattcatcgc caactcctgc aatttccagc
    15781 aactgtgccg ttgccaaacg ccagtctgcg gcttgagtaa tggcgcttac gacggcgatg
    15841 ctgccgacac ccgttgctat caccgcaggc gcgcgcgcca gactgatacc gccaatcgcc
    15901 acggtgggat aatccgccag gcgctcaaca tgccgtgcca gctgttccag cccctgcggt
    15961 gcagaaggca tctgtttggt ttgcgtcggg aacacatgtc ccagcgcgat ataagagggg
    16021 cgtgctgcca gcgcgacgtc gatctccata tcgtcatggg tcgaaacgcc cagtcgcaga
    16081 cctgccgcgc ggatggcgct gagatcggtg gcttgcaaat cttcctgccc cagatggacg
    16141 ccatatgcct gatgcttgat cgccagccgc cagtaatcgt tgataaacaa tcgcgcgtta
    16201 tagcggcggc ccagcgcaat tgccgccacg acatcggctt ccacctcttc atcgcgtcga
    16261 tctttgatgc gtagctggag agtacgtacg cctgcatcca acagacgttc gatccactgt
    16321 acgctgtcca ccaccgggta cagtcctaaa cgaaagggta caggaggaaa atctggctga
    16381 tacatcacgc ttcctcctta cgcaggtaga tttctccgcc tctggcgcgg aagttctccg
    16441 acatatcagc cattcccact tcaatggttt gcgcggcggc gtaatcgcgc acttcctggc
    16501 tgattttcat cgagcagaat ttcggcccac acatggagca aaaatgggcg actttgccgg
    16561 attcttgcgg cagggtttca tcgtgataag cgcgggcggt aaacgggtcg agggccagat
    16621 taaactggtc ttcccagcga aattcgaagc gggctttcga catggcgtta tcgcgaattt
    16681 gcgcgcccgg atgccctttc gccaggtcag cggcgtgggc ggcaatctta taggtgataa
    16741 gcccctgctt aacatcttct ttattaggca gacccagatg ctcttttggc gttacgtaac
    16801 agagcatcgc gcagccaaac cagccaatca tcgccgcacc aatccccgac gtgaagtggt
    16861 cataacccgg cgcgatatcg gtagttagcg gccccagagt gtaaaacggc gcttcgtggc
    16921 agtgctctaa ctcctcggtc atattgcggc ggatcatctg catcggcacg tggcctgggc
    16981 cttcaatcat cacctgtacg tcatattccc aggcgatttt ggtcagttca cccagcgtat
    17041 gcagctcggc aaattgcgct tcatcgttgg cgtcctgaat agaaccaggg cgcagaccgt
    17101 ctcccagcga cagcgaaacg tcataagcgg cacagatttc acaaatttcg cggaagtgtt
    17161 gatagaggaa attttcctga tgatgggaga ggcaccattt cgccataatc gaaccgccgc
    17221 gagagacgat accggtcagg cgtttcgcgg tcatcggcac atagcgcagc agtacgcccg
    17281 catggatagt gaagtaatcc acaccttgct cggcctgttc cagcagcgtg tcgcggaacg
    17341 cttcccaggt aagatcttcg gcgatcccgt taaccttctc cagcgcctgg tagatcggca
    17401 ctgtaccgat cggcaccggg ctgttacgca aaatccactc gcgggtttcg tgaatatagc
    17461 gaccggtgga gagatccatc accgtatccg ctccccagcg cgtggaccat accagctttt
    17521 ccacttcttc ttcgatggaa gaggtgaccg ccgagttgcc gatattggcg ttaactttta
    17581 ccaggaaatt gcgaccaata atcatcggct ccgattccgg atgattaatg ttggccggga
    17641 taatcgcgcg tccggcagca acttcatcac ggacaaattc cgcagtgata ttttccggca
    17701 gacgtgcgcc aaagctcatt cccggatgct ggtggcgtaa aacttcgcta cgtatgtgct
    17761 cgcggcccat attctcgcgg atggcgatga attccatttc cggtgtgatg atgccctggc
    17821 gggcgtagtg cagttgggtg acgcggcgtc ctgctttggc gcgttttggt gttagtacgc
    17881 cgctaaaacg cagctcgtcg aggccatcat ctgccagccg cgctttagtg taatcggaac
    17941 tgcgcacggt aagttcttcg gtatcgccgc gcgcatcgat ccacggctgg cgtagttttg
    18001 ccagtccttg ctgcacgtta atggcgatct gtggatcgcc atacggaccg gaggtgtcgt
    18061 agaccggaat cgcttcgttt tcttcgtact gcggttgttc tttgctgccg ccaattagcg
    18121 tcgggctaag ctggatctca cgcatcggca cgcgcacgcc ggggtgtgtg ccagtgatgt
    18181 aaatgcgttt tgagttggga aaggcggtgc cttccagggt gtcgataaaa tgttgggcct
    18241 gggcgcgttg ttcgcggcgg gtcagttttg ttacagacat agctcattcc aaaaagttaa
    18301 ggacgtggct tgtcagacga cggatgaagc aagagacgat cgccgcaaag gcgatgcgat
    18361 agcagattaa ctcttgttcc cttcgcaggt attagcctga tcaggttccg cggatcccga
    18421 ataaacggtc tcagccagtt aaggcactcc gacaagaaat tagccccaca aacggggcgt
    18481 tgaatgtaaa ttacgcgtta acagcgcaga actcaagcag gatgtttgac gcgggcggcg
    18541 tcaagtacca gcagaatcaa cttatcttcc agcacaaagc gtgcttccag cgcctcgccg
    18601 atgtcagata aaacctgttg aaactcaagg taattatcat gatcgatggc ggtttccagg
    18661 ctggaatcgt agtaatccat aatctgttgg gtattggctt cgagttgcgg ccaaatcttc
    18721 gcggcgcgcg cgagttgccc gttgccttcc agcttatgaa gaatgcgctc ataaatactg
    18781 aaatgtccgg cagacaagta atcgaccagg ctctgacaaa aatcatcaag ggctttttcg
    18841 tttagcctca tgtacgattc tttgccaggc ttaatgccaa ccagattgta gtaagccacg
    18901 agcagatgct tacgtacatg tagccagcga tcaaccagtt tgttacttcc tctgacgcgt
    18961 tccgtcaggt tatcgagctg gttaagcatg attgactccg caagtttgta ttcaaaaact
    19021 gctcagtgag aaatgtaaaa accatgttaa acatgccagt gatgcaaagg tagtgcaaga
    19081 gctatggatc gtataattga aaaattagat cacggctggt gggtcgtcag ccatgaacaa
    19141 aaattatggt tgccgaaggg agaattgcca tatggcgaag cggcaaattt cgatcttgtg
    19201 ggtcagcgcg cactacaaat cggcgaatgg caaggggaac ctgtttggtt agtacaacag
    19261 cagcggcgtc acgatatggg gtcggtacgt caggtcattg atctcgatgt agggctgttt
    19321 caactggccg gacgtggcgt gcaactggcg gagttttacc gatcgcataa atactgtggt
    19381 tactgcgggc atgaaatgta tccaagcaaa accgaatggg cgatgctgtg cagccattgc
    19441 cgtgagcgtt actacccgca aatcgccccc tgcattattg ttgccatccg tcgcgatgat
    19501 tcgatcctcc tcgcccagca tacccgccat cgtaacggtg tccatacagt acttgccgga
    19561 ttcgtcgaag tgggtgaaac cctcgagcag gcagtcgcgc gggaagtgat ggaagagagc
    19621 ggaattaaag ttaaaaactt gcgttacgtg acctcccaac cgtggccgtt tcctcagtcg
    19681 ttaatgaccg cgtttatggc ggaatatgac agcggcgaca tcgttatcga cccgaaagaa
    19741 ttgctcgagg cgaactggta tcgctatgac gatttgccgt tactcccgcc gcccggcacc
    19801 gtagcgcgcc gtctgataga agatacggtg gcgatgtgtc gggcagagta tgagtgatga
    19861 tacactgacc gcctgacgca ctaaggaaca gccaaaatga ccgaacttaa aaacgatcgt
    19921 tatctgcggg cgctgctgcg ccagcccgtt gatgtcactc cagtatggat gatgcgccag
    19981 gcgggtcgct atctaccgga atataaagcc acgcgcgccc aggcgggcga ttttatgtcg
    20041 ctgtgcaaaa atgccgagct ggcgtgcgaa gtgactttgc aaccgctgcg tcgctatccg
    20101 ctggatgcgg cgatcctctt ttccgatatc ctcaccgtgc cagacgcgat gggtttaggg
    20161 ctctattttg aagccggaga aggtccgcgt tttacctcac cagtcacctg caaggccgac
    20221 gtcgataaac tgccaattcc ggacccggaa gatgagctgg gttacgtgat gaacgcggtg
    20281 cgtaccattc gtcgcgaact gaaaggcgaa gtgccgctga ttggtttttc cggcagcccg
    20341 tggacgctgg cgacctatat ggtggaaggc ggcagcagca aagcgttcac cgtgatcaaa
    20401 aaaatgatgt atgccgatcc acaggcgctg cacgcgctac tcgataaact ggcgaaaagc
    20461 gtcactttgt atctgaatgc gcagattaaa gccggtgctc aggctgtgat gattttcgac
    20521 acctggggcg gcgtgcttac cgggcgcgat tatcaacagt tctcgctcta ttacatgcat
    20581 aaaattgttg atggtttact gcgtgaaaac gacggtcgcc gcgtaccggt cacgctgttt
    20641 accaaaggcg gcggacagtg gctggaagcc atggcagaaa ccggttgcga tgcgctgggc
    20701 ctcgactgga caacggacat cgccgatgca cgccgccgcg tgggcaataa agtcgcgttg
    20761 cagggcaata tggatccgtc gatgctgtac gcgccgcctg cccgcattga agaagaagta
    20821 gcgactatac ttgcaggttt cggtcacggt gaaggtcatg tctttaacct tggtcacggc
    20881 attcatcagg atgtgccgcc agaacatgct ggcgtgtttg tggaggcagt gcatcgcctg
    20941 tctgcgcctt atcatctgta aggagtggtt atggatctcg cgtcattacg cgctcaacaa
    21001 atcgaactgg cttcttctgt gatccgcgag gatcgactcg ataaagatcc accggatctg
    21061 atcgccggag ccgatgtcgg atttgagcag ggcggagaag tgacgcgagc ggcgatggtg
    21121 ctgctgaaat acccctcgct tgagctggtg gagtataaag tggcccgcat cgccaccacc
    21181 atgccttaca ttcctggttt tctctccttt cgtgaatatc ctgcgctgct ggcagcgtgg
    21241 gagatgctgt cacaaaagcc ggatttagtg tttgtcgatg gtcatgggat ctcgcatcct
    21301 cgccgtcttg gcgtcgccag ccattttggt ttgctggtgg atgtgccgac cattggcgta
    21361 gcgaaaaaac ggctctgcgg taaattcgaa ccgctctcca gcgaaccggg cgcgctggcc
    21421 ccgctgatgg ataaaggcga gcagctggcc tgggtctggc gcagcaaagc gcgctgtaac
    21481 ccattgttta tcgctaccgg ccatcgggtc agcgtggaca gcgcgctggc gtgggtacaa
    21541 cgctgcatga aaggctatcg tctgccggag ccaacgcgct gggcggacgc ggtggcctcg
    21601 gaacgtccgg cgttcgtgcg ctatacagca aatcagccct aattcaggta aactgcggat
    21661 aatttccgta tttgagaact caacatgtta caaaacccaa ttcatctgcg tctggagcgc
    21721 ctggaaagct ggcagcacgt cacttttatg gcctgcttat gcgaacgtat gtaccccaat
    21781 tacgccgtat tctgtcagca aaccggcttc ggcgatgggc agatttaccg ccgcattctc
    21841 gatctcattt gggaaacgct gaccgtcaaa gacgcaaaag taaatttcga cagccaactg
    21901 gagaaatttg aagaagcgat tccttcagcc gacgatttcg atctgtacgg cgtttatccg
    21961 gcaatcgatg cttgcgtggc gttaagtgaa ctggtccatt cgcgtttaag tggcgaaacg
    22021 ctcgaacacg cggtggaagt gagtaagact tccatcacga ccgtggcgat gctggaaatg
    22081 actcaggctg gtcgcgaaat gagcgatgaa gagctcaaag aaaacccagc tgtagagcaa
    22141 gaatgggaca ttcagtggga aatattccga cttttagctg agtgcgaaga acgcgatatc
    22201 gagctgataa aaggccttag ggcagacctg cgtgaggcgg gtgagagcaa tattggtata
    22261 atttttcagc aataagacca gaaaacgtga tttaacgcct gatttgtcgt acctggagtc
    22321 ttccctttcg ccccccgtct ggtctacatt tggggggcga aaaaaagtgg ctatcggtgc
    22381 gtgtatgcag gagagtgcta ttctggcatt tccgtcgcac tcgatgctta gcaagcgata
    22441 aacacattgt aaggataact tatgaacaag actcaactga ttgatgtaat tgcagagaaa
    22501 gcagaactgt ccaaaaccca ggctaaagct gctctggagt ccactctggc tgcaattact
    22561 gagtctctga aagaaggcga tgctgtacaa ctggttggtt tcggtacctt caaagtgaac
    22621 caccgcgctg agcgtactgg ccgcaacccg cagaccggta aagaaatcaa aattgccgca
    22681 gctaacgtac cggcatttgt ttctggcaag gcactgaaag acgcagttaa gtaagattgc
    22741 gtggcagtga acagttttaa cgaaggggtg gtttcacccc ttttgtcttt ctggcgtcga
    22801 tcattgatgc tggctggcgc tctgtttctc actgcttgta gtcacaactc ttcacttcct
    22861 ccctttaccg ccagtggatt tgctgaagac cagggcgcgg tacgcatctg gcgaaaagac
    22921 agcggcgata atgtgcatct tcttgccgtg tttagcccgt ggcgcagtgg cgatacgacg
    22981 acgcgagagt atcgctggca gggcgataac ctcacgctca tcaatatcaa tgtttacagc
    23041 aaacccccgg tgaatattcg cgcgcgtttt gacgatcgcg gcgatctgag ctttatgcaa
    23101 cgcgaatccg atggggaaaa gcagcagctt tctaacgacc aaatcgattt ataccgttat
    23161 cgtgctgatc agatccgcca gattagcgat gccttacgtc aggggagagt cgtgctgcgc
    23221 caggggcgct ggcatgcgat ggagcagact gtgaccacct gcgaagggca aaccattaaa
    23281 cctgatttag attcgcaggc gatagcgcat atcgagcgcc gccagagccg ctcttctgtt
    23341 gatgtcagcg tggcatggct ggaagcgccc gaaggttcgc aattactgtt ggtggcaaac
    23401 tctgatttct gtcgctggca acccaacgag aaaacgttct gatttaccag tggtccatac
    23461 ccatatgacc gccaccaccg cagccgccgt aacccattcc ggtaccgcgc ggaatacccg
    23521 cttcagccat cgcaatatct cgtttcaccc gtaactcatc taacgactga cgcaaattct
    23581 ccatctcttt ggcgaccgcg ttaattttgc tgctatccgg tgggttcgcg gctaacaggg
    23641 cattgtattc ataacgcttt gttaccagtt gctgttgcag tgcgctgctt tgagcgtaaa
    23701 agtcattatg gattttctgc cacgctgtct gttgttcgct ggtcaaaggc gcggcatttt
    23761 gctgccacat accgtgtccg ccgtgagcaa atgccgatgt cgatcccatc gccattgctg
    23821 aaagcgccat cattaccagg gcaattttcg tgttccgttt catggttaat cctccagtgg
    23881 ttgtcttact tcgggtattg catcttccgt gccaacgatg aaacgctgat atgacgggta
    23941 atctggcatg ataaacgagt aaaaatgact cgcctgctgc gggtagcgag tcatttttac
    24001 tcattgaaac ttaagccttt gtgttacagc gcagggtaag cgctgataaa agatggcatg
    24061 atttctgctg tcagaaaggg atgagcaggc aaagaagaag atgcgtttta tgcaacgttc
    24121 taaagactcc ttagctaaat ggttaagcgc gatcctcccc gtggtcattg ttgggctggt
    24181 ggggctgttt gcggtgacag tgattcgcga ttacgggcgc gagactgcag ccgccagaca
    24241 aacgctgctg gaaaaaggca gtgtacttat ccgtgctctc gaatccggct cgcgcgtcgg
    24301 catggggatg cgcatgcatc atgcgcagca gcaggcatta ctggaagaaa tggccgggca
    24361 gcctggtgta cgttggtttg cggtcacgga tgaacaagga acaatcgtga tgcatagcaa
    24421 ctccggcatg gtgggaaaac agctttattc cccgcaggaa atgcagcagt tacatccggg
    24481 agatgaagaa gcgtggcggc ggatcgatag cgcagacggc gagcctgttc tggaaattta
    24541 tcgccagttt caaccgatgt ttgctgctgg aatgcaccgg atgcgccata tgcagcagta
    24601 tgccgcgaca ccacaagcaa tttttatcgc tttcgacgcc agtaacattg tgagtgccga
    24661 agatcgtgag cagagaaaca ccctgattat cctcttcgcc ctggcgacgg tcttgctggc
    24721 aagcgtgttg tcattcttct ggtatcgccg atatctgcgc tcgggccagc tgttgcagga
    24781 tgaaatgaag cgcaaagaga agctggtggc actggggcat cttgcggcag gcgttgccca
    24841 cgaaatccgt aacccacttt cctcgattaa agggctggcg aaatactttg ccgaacgcgc
    24901 gccagcaggg ggagaagcgc atcaactggc gcaggtgatg gcgaaagaag ccgaccgttt
    24961 aaaccgcgtg gtaagcgagt tgctggaact ggttaagcca acgcatctgg ctttgcaggc
    25021 ggtggatctc aacacgctga ttaaccactc attacagctg gtaagccagg atgcaaacag
    25081 ccgggagatc cagttacgct ttaccgccaa cgacacatta ccggaaattc aggccgatcc
    25141 ggacaggctg actcaggtcc tgttgaatct ctatctcaat gctattcagg cgattggtca
    25201 gcatggcgtg attagcgtga cggccagcga aagcggcacg ggtgtgaaaa tcagcgttac
    25261 cgacagcggt aagggaattg cggcggatca gcttgaagcc atcttcaccc cgtatttcac
    25321 cacgaaggcc gaaggcaccg gactggggct ggcggtcgtg cataatattg ttgaacaaca
    25381 cggtggtaca attcaggtcg caagccaggt gggaaaaggc gcaacgttca ccctctggct
    25441 tccggtcact attacgcgta aggacccaca aggatgacgc acgataatat cgatattctg
    25501 gtggtggatg atgacattag ccactgcact attttgcagg ctttgctgcg cggctggggc
    25561 tataacgtcg cgctggcgaa cagcgggcga caggcgctgg agcaggtgcg ggaacaggtt
    25621 tttgatcttg tgctttgcga tgtgcgaatg gcggagatgg acggcattgc cacgctaaaa
    25681 gagatcaaag cgctaaatcc ggcgattccg gtactgatta tgaccgcata ctccagcgtc
    25741 gagacggcgg tagaggcact gaaaaccggg gcgctggatt atctcatcaa gccgctggat
    25801 ttcgataacc tgcaggcgac gctggaaaag gcgctcgcac atacgcacag tgttgacgct
    25861 gaaacgcctg cggtgtctgc cagccagttc ggtatggtag gtaaaagccc ggcgatgcaa
    25921 cacctgctca gtgaaatcgc cctcgtcgcg ccatcggaag ccacagtgct gatccacggc
    25981 gattccggca ccggtaaaga gctggtcgca agggcgattc acgccagtag cgcacggagt
    26041 gaaaaaccac tggtaacgct caactgtgcg gcactcaacg aatccttgct ggaatctgaa
    26101 ttgttcggtc acgaaaaagg ggcgtttacc ggagccgata aacgccggga agggcgcttt
    26161 gttgaggcgg acggcggcac gctgtttctc gatgaaattg gcgatatctc gccgatgatg
    26221 caggttcgtc tgctgcgtgc gattcaggag cgcgaagttc agcgtgtcgg cagcaaccag
    26281 accatctcgg ttgatgtccg gctgattgcg gcgactcatc gcgatcttgc cgcagaggtg
    26341 aatgccgggc gttttcgcca ggatctctac tatcgcctga acgtggtggc gattgaagtt
    26401 ccgtcgctgc gtcagcggcg ggaagatatt cctctgctgg caaaccattt tcttcagcgc
    26461 tttgccgagc gtaatcgtaa ggcggtaaaa ggttttacgc cccaggcgat ggatctgctg
    26521 attcactacg actggccggg aaatattcgt gaactggaaa acacggtgga acgggcggtg
    26581 gtgctgctga ccggggaata tatttccgaa cgcgagctgc cgctggcgat tgcgagtacg
    26641 ccgatcccgc tggtacaaag tcaggatatt cagccgctgg tggaagtcga aaaagaggtg
    26701 attctggcgg cgctggagaa aacgggcggc aacaaaaccg aagccgcccg ccagttaggg
    26761 atcacgcgca aaacgctatt ggcaaaactg tcgcgttagt tctgctcgcg ttcgatagcg
    26821 cgccagccga tatctttccg gcagaagcag tcgtcccaat ggatatcggt cattaaggca
    26881 taagcgcgtt tctgcgcttc tgccacggta tgacccagcg cggtaacgca cagtacgcgc
    26941 ccgccgctgg tcactacctg ctcgtcatcc gctagtttcg tgcccgcgtg gaacactttg
    27001 ccgtctgcca cttcttccag cggcaggccg tggatcacat cgccggtgcg gtaatcgccc
    27061 ggatatccac ccgcagccat caccacgccg agagaagcgc gttcatccca ctcggacgtt
    27121 ttctcgtcca gcttgccttc acaggccgcc aggcagagct caaccagatc ggacttcatg
    27181 cgcagcataa tcggctgggt ttctggatcg ccaaagcggc agttaaattc aataaccttc
    27241 ggattgccct gtttgtcgat catcaggccc gcgtagagaa aaccagtgta ggtgttgcct
    27301 tctgccgcca tgcctttcac ggttggccag atgattcgtt ccatcgtgcg ctgatgaact
    27361 tcatcggtta ctaccggcgc gggggagtaa gcgcccatcc cgcccgtgtt tggtccggta
    27421 tctttatcgc ctacgcgttt gtgatcctgg ctggtagcca tcggcagcac atgctcgccg
    27481 tccaccatca cgataaagct cgcttcttcg ccatcgagga actcttcgat aacgatgcga
    27541 tgacccgcgt cgccaaacgc gttgcccgcc agcatatcgt gaacagctgc ttcagcttct
    27601 tccagcgtca tcgccacgat aacgcctttc ccggctgcca gaccgtcagc tttaatgacg
    27661 attggcgcgc ctttctcacg cagatacgcc agcgcaggtt ctacctcggt gaagttctgg
    27721 tattccgctg tgggaatgtt atggcgggcc agaaagtctt tggtgaatgc tttagaacct
    27781 tccagttgtg cggcacctgc ggttgggccg aagattttca gcccggcggc gcggaaggta
    27841 tcgactacgc ctttcaccag cggcgcttcc gggccgacga tggttagatc gatcttttcg
    27901 ttttgcgcga aatccagcag cgccgggata tcggttacgc cgatagcgac gttttgcagt
    27961 gtgggttcca gcgcagtgcc tgcattaccc ggagcaacaa aaacagtttc aaccagcggc
    28021 gactgggccg ctttccaggc cagcgcgtgc tcgcgcccgc cgttaccaat cactaatact
    28081 ttcatctgtc gctccattaa tggcggaagt ggcgcatgtc ggtgaagagc atcgcaatac
    28141 cgtgctcgtc ggcggcggca atcacttcgt catcacggat agaaccgcca ggctggatta
    28201 cgcaggtcac acccgcagcg gcggcggcat caataccgtc gcggaacggg aagaacgcgt
    28261 cagaagccat cgaggaacct ttcacttcca ggccttcatc ggccgcttta ataccggcga
    28321 tttttgcgga gtacacgcgg ctcatctggc ccgcgccaat gccgatggtc atattgtttt
    28381 tggcatagac aatggcgttg gatttcacga acttcgccac tttccagcag aacagcgcat
    28441 cacgcagttc ctgttcggtc ggctgacgtt tggtgacgac gcgcagttct tctgcaccga
    28501 ccatgcccag gtcgcgatcc tgaaccagca gaccgccgtt aacacgtttg aaatcaagac
    28561 ccggaacacg ctcgccccac tgaccgcagg tcaggacgcg tacgttctgt ttggcggcgg
    28621 tgattttcag ggcttcttcg ctggcggacg gcgcaataat cacttcaaca aactgacgag
    28681 aaatgatggc ctgcgcggtt tccgcatcca gctcgcggtt aaaggcgata atgccgccga
    28741 atgcggaggt cgggtcggtt ttgtacgcgc gatcgtaagc atcaagaatg gaattgctga
    28801 tagccacgcc gcacgggttg gcgtgcttca caatcacaca tgctggctcg gcgaactctt
    28861 tcacgcactc cagcgccgca tcggtatcgg cgatgttgtt ataagagagg gccttaccct
    28921 gaacctgggt tgcggtggca acagaggctt ctttcacgtt ctcttctata tagaaggcag
    28981 cctgctggtg gctgttctcg ccgtaacgca tatcctgctt cttaatgaag ttcaggttca
    29041 gcgtgcgtgg gaagcgaccg gcggcttctt tgctttcacc gtggtaagcc ggaaccatgc
    29101 tgccgaagta gttggcaatc atgctgtcgt aggcggcggt atgttcaaag gctttgatag
    29161 cgagatcgaa acgggttgca agcagaagcg atccttcgtt ggcatccatc tctttaatga
    29221 tggcgtcgta gtcgctgctc ttcaccacga tggcgacatc tttatggttc ttggcggcag
    29281 agcgcaccat cgtcgggccg ccgatatcga tgttctcaac cgcatcttcc agcgagcaac
    29341 cttcacgggc aacggtctgg gcgaacggat acaggttaac aaccaccata tcgataggct
    29401 ggatctgatg ttcttccata atggcatcgt cctggccgcg acggcccaga atgccaccat
    29461 gtactttcgg atgcagggtc ttcacgcgtc catccatcat ctccgggaaa ccggtgtaat
    29521 cggaaacttc ggttaccggc agaccttttt ctgctaacag acgggctgtg ccccctgtag
    29581 acagcagctc cacaccgcgt gcggaaagtg cctgggcgaa ttcgacgata ccggctttgt
    29641 cagaaacact gagcagagcg cggcggactg gacgacgttg ttgcatggta aatcccctgg
    29701 atttgactat tacagagagc gttagctgaa tttttcgcga aaaactcagc taacgcccct
    29761 aacggggcat ccttattttt cgcccgcatt gtaacgaaaa cgtttgcgca acgctcgcga
    29821 atttttctct ttcaatggtg atcacaattt tgacagtggc taccgtggga aaaatacaga
    29881 aattacattg atgattgtgg ataactctgt gcgtaaaaag gtataaagcg gggttttgct
    29941 ggggaatgca gcagtcagtc atttttatgc aatttttcta ttgcgacctg cggagaactc
    30001 cctataatgc gcctccatcg acacggcgga tgtgaatcac ttcacacaaa cagccggttc
    30061 ggttgaagag aaaaatcctg aaattcaggg ttgactctga aagaggaaag cgtaatatac
    30121 gccacctcgc gacagtgagc tgaaagccgc gtcgca
//
LOCUS       DAZFLD010000033        29525 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.033, whole genome shotgun sequence.
ACCESSION   DAZFLD010000033 DAZFLD010000000
VERSION     DAZFLD010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29525
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.033"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..546)
                     /locus_tag="ACHKBL_001275"
     CDS             complement(<1..546)
                     /locus_tag="ACHKBL_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003843851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFO5818168.1"
                     /translation="MIIEKVMNNNCVQASMNGQEVIISGPGVGYNKKYGMSVPEHPAN
                     RIFYVRNEQKNKLYKLIEHVDVEYIFVAEKIVQYAEKNLEKNLNPSLLLILADHISNA
                     ISRVVSGIQINNVFLDEIKALYKAEYAISRDALTIINEQFSVQLPDDEIGFIALHILN
                     NYENSVDYESVRIIELSQIITE"
     gene            complement(804..2057)
                     /gene="amiC"
                     /locus_tag="ACHKBL_001276"
     CDS             complement(804..2057)
                     /gene="amiC"
                     /locus_tag="ACHKBL_001276"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311701.2"
                     /note="Like AmiA, dependent on TAT (Twin-Arginine
                     Translocation) system for export; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiC"
                     /protein_id="HFO5818169.1"
                     /translation="MSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPA
                     SSYTRVTVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSAR
                     VGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
                     YNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQI
                     ARRLRSLIEKEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSG
                     SSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLK
                     FGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQ
                     QEVAESILAGIKAYFADGATLARRG"
     gene            2289..3620
                     /gene="argA"
                     /locus_tag="ACHKBL_001277"
     CDS             2289..3620
                     /gene="argA"
                     /locus_tag="ACHKBL_001277"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004042 - acetyl-CoA:L-glutamate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HFO5818170.1"
                     /translation="MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSS
                     IVNDIGLLHSLGIRLVVVYGARPQIDANLAAHHHEPLYHKNIRVTDAKTLELVKQAAG
                     TLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
                     IHRQLDSGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDD
                     GDIVSELFPNEAQARVEAQEEKGDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGAL
                     LQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQGILVRRSREQLEMEI
                     DKFTIIQRDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERIAAQAKQS
                     GLSKLFVLTTRSIHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG"
     gene            complement(3858..5684)
                     /gene="recD"
                     /locus_tag="ACHKBL_001278"
     CDS             complement(3858..5684)
                     /gene="recD"
                     /locus_tag="ACHKBL_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HFO5818171.1"
                     /translation="MKLQKQLLEAVEHKQLRPLDVQFALTVAGDEHPAVTLAAALLSH
                     DAGEGHVCLPLSRLENNEASHPLLATCVSEIGELQNWEECLLASQAVSRGDEPTPMIL
                     CGDRLYLNRMWCNERTVARFFNEVNHAIEVDEALLAQTLDKLFPVSDEINWQKVAAAV
                     ALTRRISVISGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGK
                     ALRQLPLTDEQKKRIPEDASTLHRLLGAQPGSQRLRHHAGNPLHLDVLVVDEASMIDL
                     PMMSRLIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTAERAGQLSRLTG
                     THVPSGTGTEAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKTAVKTVFQQDFTD
                     IEKRLLQSGEDYIAMLEEALAGYGRYLDLLQARAEPDLIIQAFNEYQLLCALREGPFG
                     VAGLNERIEQFMQQKRKIHRNPHSRWYEGRPVMIARNDSALGLFNGDIGIALDRGQGT
                     RVWFAMPDGNIKSVQPSRLPEHETTWAMTVHKSQGSEFDHAALILPSQRTPVVTRELV
                     YTAVTRARRRLSLYADERILSAAIATRTERRSGLAALFSSRE"
     gene            complement(5684..9226)
                     /gene="recB"
                     /locus_tag="ACHKBL_001279"
     CDS             complement(5684..9226)
                     /gene="recB"
                     /locus_tag="ACHKBL_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HFO5818172.1"
                     /translation="MSDVAETLDPLRLPLQGERLIEASAGTGKTFTIAALYLRLLLGL
                     GGSAAFPRPLTVEELLVVTFTEAATAELRGRIRSNIHELRIACLRETTDNPLYKLLLE
                     EIDDKAQAAQWLLLAERQMDEAAVFTIHGFCQRMLNLNAFESGMLFEQQLIEDESLLR
                     YQACADFWRRHCYPLPREIAQVVFETWKGPQALLRDINRYLQGEAPVIKAPPPDDETL
                     ASRHAQIVARIDAVKQQWRDAVGELDALIESSGIDRRKFNRSNQAKWIDKISAWAEEE
                     TNSYQLPESLEKFSQRFLEDRTKAGGETPRHPLFEAIDQLLAEPLSIRDLVITRALAE
                     IRETVAREKRRRGELGFDDMLSRLDSALRSESGEVLAAAIRTRFPVAMIDEFQDTDPQ
                     QYRIFRRIWHHQPETALLLIGDPKQAIYAFRGADIFTYMKARSEVHAHYTLDTNWRSA
                     PGMVNSVNKLFSQTDDAFMFREIPFIPVKSAGKNQALRFVFKGETQPAMKMWLMEGES
                     CGVGDYQSTMAQVCAAQIRDWLQAGQRGEALLMNGDDARPVRASDISVLVRSRQEAAQ
                     VRDALMLLEIPSVYLSNRDSVFETLEAQEMLWLLQAVMTPERENTLRSALATSMMGLN
                     ALDIETLNNDEHAWDAVVEEFDGYRQIWRKRGVMPMLRALMSARNIAENLLATAGGER
                     RLTDILHISELLQEAGTQLESEHALVRWLSQHILEPDSNASSQQMRLESDKHLVQIVT
                     IHKSKGLEYPLVWLPFITNFRVQDQAFYHDRHSFEAVLDLNAAPESVDLAEAERLAED
                     LRLLYVALTRSVWHCSLGVAPLVRRRGDKKGDTDVHQSALGRLLQKGEPQDAAGLRTC
                     IEALCDDDIAWQTAQTGDNQPWQVNDAITAELNARTLQRLPGDNWRVTSYSGLQQRGH
                     GIAQDLMPRLDVDAAGVVSVVDEPTLTPHQFPRGASPGTFLHSLFEDLDFTQPVDPNW
                     VQEKLELGGFESQWEPVLTEWITAVLQAPLNETGVSLSQLSDRDKQVEMEFYLPISEP
                     LIASQLDALIRQFDSLSAGCPPLEFMQVRGMLKGFIDLVFRHEGRYYLLDYKSNWLGE
                     DSSAYTQQAMAAAMQAHRYDLQYQLYTLALHRYLRHRIADYDYERHFGGVIYLFLRGV
                     DKEHPQQGIYATRPNAGLIDLMDEMFAGITLEEA"
     gene            complement(9219..12107)
                     /gene="ptrA"
                     /locus_tag="ACHKBL_001280"
     CDS             complement(9219..12107)
                     /gene="ptrA"
                     /locus_tag="ACHKBL_001280"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HFO5818173.1"
                     /translation="MPRSTWFKALLLFVALWAPLSQAETGWQPIQETIRKSDKDNRQY
                     QAIRLDNGMVVLLVSDPQAVKSLSALVVPVGSLEDPEAYQGLAHYLEHMSLMGSKKYP
                     QADSLAEYLKMHGGSHNASTAPYRTAFYLEVENDALPGAVDRLADAIAEPLLDKKYAE
                     RERNAVNAELTMARTRDGMRMAQVSAETINPAHPGSKFSGGNLETLSDKPGNPVQQAL
                     KDFHEKYYSANLMKAVIYSNKPLPELAKMAADTFGRVPNKESKKPEITVPVVTDAQKG
                     IIIHYVPALPRKVLRVEFRIDNNSAKFRSKTDELITYLIGNRSPGTLSDWLQKQGLVE
                     GISANSDPIVNGNSGVLAISASLTDKGLANRDQVVAAIFSYLNLLREKGIDKQYFDEL
                     ANVLDIDFRYPSITRDMDYVEWLADTMIRVPVEHTLDAVNIADRYDAKAVKERLAMMT
                     PQNARIWYISPKEPHNKTAYFVDAPYQVDKISAQTFADWQKKAADIALSLPELNPYIP
                     DDFSLIKSEKKYDHPELIVDESNLRVVYAPSRYFSSEPKADVSLILRNPKAMDSARNQ
                     VMFALNDYLAGLALDQLSNQASVGGISFSTNANNGLMVNANGYTQRLPQLFQALLEGY
                     FSYTATEDQLEQAKSWYNQMMDSAEKGKAFEQAIMPAQMLSQVPYFSRDERRKILPSI
                     TLKEVLAYRDALKSGARPEFMVIGNMTEAQATTLARDVQKQLGADGSEWCRNKDVVVD
                     KKQSVIFEKAGNSTDSALAAVFVPTGYDEYTSSAYSSLLGQIVQPWFYNQLRTEEQLG
                     YAVFAFPMSVGRQWGMGFLLQSNDKQPSFLWERYKAFFPTAEAKLRAMKPEEFAQIQQ
                     AVITQMLQAPQTLGEEASKLSKDFDRGNMRFDSRDKIVAQIKLLTPQKLADFFHQAVV
                     EPQGMAILSQISGSQNGKAEYVHPEGWKVWENVSALQQTMPLMSEKNE"
     gene            complement(12283..15651)
                     /gene="recC"
                     /locus_tag="ACHKBL_001281"
     CDS             complement(12283..15651)
                     /gene="recC"
                     /locus_tag="ACHKBL_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HFO5818174.1"
                     /translation="MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMILVQSTGMAQ
                     WLQMTLSQKFGIAANIDFPLPASFIWDMFVRVLPEIPKESAFNKQSMSWKLMTLLPQL
                     LEREDFTLLRHYLTDDSDKRKLFQLSSKAADLFDQYLVYRPDWLAQWEIGHLVEGLGE
                     AQAWQAPLWKALVEYTHELGQPRWHRANLYQRFIETLESATTCPPGLPSRVFICGISA
                     LPPVYLQALQALGKHIEIHLLFTNPCRYYWGDIKDPAYLAKLLTRQRRHSFEDRELPL
                     FRDSENAGQLFNSDGEQDVGNPLLASWGKLGRDYIYLLSDLESSQELDAFVDVTPDNL
                     LHNIQSDILELENRAVAGVNIEEFSRSDNKRPLDPLDSSITFHVCHSPQREVEVLHDR
                     LLAMLEEDPTLTPRDIIVMVADIDSYSPFIQAVFGSAPADRYLPYAISDRRARQSHPV
                     LEAFISLLSLPDSRFVSEDVLALLDVPVLAARFDITEEGLRYLRQWVNESGIRWGIDD
                     DNVRELELPATGQHTWRFGLTRMLLGYAMESAQGEWQSVLPYDESSGLIAELVGHLAS
                     LLMQLNIWRRGLAQERPLEEWLPVCRDMLNAFFLPDAETEAAMTLIEQQWQAIIAEGL
                     GAQYGDAVPLSLLRDELAQRLDQERISQRFLAGPVNICTLMPMRSIPFKVVCLLGMND
                     GVYPRQLAPLGFDLMSQKPKRGDRSRRDDDRYLFLEALISAQQKLYISYIGRSIQDNS
                     ERFPSVLVQELIDYIGQSHYLPGDEALNCDESEARVKAHLTCLHTRMPFDPQNYQPGE
                     RQSYAREWLPAASQAGKAHSEFVQPLPFTLPETVPLETLQRFWAHPVRAFFQMRLQVN
                     FRTEDSEIPDTEPFILEGLSRYQINQQLLNALVEQDDADRLFRRFRAAGDLPYGAFGE
                     IFWETQCQEMQQLADRVIARRQPGQSMEIDLACNGVQITGWLPQVQPDGLLRWRPSLL
                     SVAQGMQLWLEHLVYCASGGNGESRLFLRKDGEWRFPPLAAEQALHYLSQLIEGYREG
                     MSAPLLVLPESGGAWLKTCYDAQNDAMLDDDSTLQKARTKFLQAYEGNMMVRGEGDDI
                     WYQRLWRQLTPETMEAIVEQSQRFLLPLFRFNQS"
     gene            complement(15664..15987)
                     /gene="ppdC"
                     /locus_tag="ACHKBL_001282"
     CDS             complement(15664..15987)
                     /gene="ppdC"
                     /locus_tag="ACHKBL_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HFO5818175.1"
                     /translation="MSASLRNQQGFSLPEVMLAMVLMVMIVTALSGFQRTLMNSLASR
                     NQYQQLWRHGWQQTQLRAISPPANWQVNRMQTSQAGCVSISVTLVSPGGREGEMTRLH
                     CPNRQ"
     gene            complement(15972..16379)
                     /gene="ygdB"
                     /locus_tag="ACHKBL_001283"
     CDS             complement(15972..16379)
                     /gene="ygdB"
                     /locus_tag="ACHKBL_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311708.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2509 family protein"
                     /protein_id="HFO5818176.1"
                     /translation="MNREKGVSSLALVLMLLILGSLLLQGMSQQDRSFASRVSMESQS
                     LRRQAIVQSALEWGKMHSWQTLPAVQCLLYAATGARVCLRLLADNEALLIAGYEGVSL
                     WRTGEVIDGNIVFSPRGWSDFCPLKERALCQLP"
     gene            complement(16376..16939)
                     /gene="ppdB"
                     /locus_tag="ACHKBL_001284"
     CDS             complement(16376..16939)
                     /gene="ppdB"
                     /locus_tag="ACHKBL_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HFO5818177.1"
                     /translation="MPVKEQGFSLLEVLIAMAISSVLLLGAARFLPALQRESLTNTRK
                     LALEDEIWLRVFTVAKHLQRAGYCHGSCTGEGLEIVGQGDCVIVQWDANSNGIWDREP
                     VKESDQIGFRLKEHVLETLRGATSCEGKGWDKVTNPDAIIIDTFQVVRQDVSGFSPVL
                     TVNMHAASKADPQTVVDASYSVTGSNL"
     gene            complement(16930..17400)
                     /gene="ppdA"
                     /locus_tag="ACHKBL_001285"
     CDS             complement(16930..17400)
                     /gene="ppdA"
                     /locus_tag="ACHKBL_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HFO5818178.1"
                     /translation="MKTQRGYTLIETLVAMLILVMLSASGLYGWQYWQQSQRLWQTAS
                     QARDYLLYLREDANWHNRDHSISLIREGTLWCLVSSVAGANTCHGSSPLVFVPRWPEV
                     EMSDLTPSLAFFGLRNTAWAGHIRFKNSTGEWWLVVSPWGRLRLCQQGETEGCL"
     gene            complement(17585..18379)
                     /gene="thyA"
                     /locus_tag="ACHKBL_001286"
     CDS             complement(17585..18379)
                     /gene="thyA"
                     /locus_tag="ACHKBL_001286"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFO5818179.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPL
                     VTTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWP
                     TPDGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
                     CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHL
                     QLSREPRPLPKLIIKRKPESIFDYRFEDFEIEGYDPHPGIKAPVAI"
     gene            complement(18386..19261)
                     /gene="lgt"
                     /locus_tag="ACHKBL_001287"
     CDS             complement(18386..19261)
                     /gene="lgt"
                     /locus_tag="ACHKBL_001287"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HFO5818180.1"
                     /translation="MTSSYLHFPEFDPVIFSIGPVALHWYGLMYLVGFIFAMWLATRR
                     ANRPGSGWTKNEVENLLYAGFLGVFLGGRIGYVLFYNFPQFMADPLYLFRVWDGGMSF
                     HGGLIGVIVVMIIFARRTKRSFFQVSDFIAPLIPFGLGAGRLGNFINGELWGRVDPNF
                     PFAMLFPGSRTEDILLLQTNPQWQSIFDTYGVLPRHPSQLYELLLEGVVLFIILNLYI
                     RKPRPMGAVSGLFLIGYGAFRIIVEFFRQPDAQFTGAWVQYISMGQILSIPMIVAGVI
                     MMVWAYRRSPQQHVS"
     gene            complement(19412..21658)
                     /gene="ptsP"
                     /locus_tag="ACHKBL_001288"
     CDS             complement(19412..21658)
                     /gene="ptsP"
                     /locus_tag="ACHKBL_001288"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFO5818181.1"
                     /translation="MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLAD
                     HDRRCYYLMATRGLKKPRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSV
                     KEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQLT
                     ALFGQYRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEE
                     AANEFRRYSKRFAAGAQKETAAIFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVI
                     EKFAEQFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWPERFILVADELSATT
                     LAELPQDRLVGVVVRDGAANSHAAIMVRALGIPTVMGADIQPSVLHRRTLIVDGYRGE
                     LLVDPEPVLLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSPEHEEK
                     LGSRIDGIGLYRTEIPFMLQSGFPSEEEQVAQYQGMLQMFNDKPVTLRTLDVGADKQL
                     PYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVTSLDEVD
                     EARRLIERAGREVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDFISVGTNDLTQ
                     YILAVDRNNTRVANIYDSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMCVAILI
                     GLGYRHLSMNGRSVARVKYLLRRIDFAEAENLAQRSLEAQLATEVRHQVAAFMERRGM
                     GGLIRGGL"
     gene            complement(21671..22201)
                     /gene="rppH"
                     /locus_tag="ACHKBL_001289"
     CDS             complement(21671..22201)
                     /gene="rppH"
                     /locus_tag="ACHKBL_001289"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HFO5818182.1"
                     /translation="MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGINPGE
                     SAEQAMYRELFEEVGLSRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLL
                     QLVSGDAEINMQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQE
                     NTPKPQNASAYRRKRG"
     gene            complement(22554..22700)
                     /gene="ygdT"
                     /locus_tag="ACHKBL_001290"
     CDS             complement(22554..22700)
                     /gene="ygdT"
                     /locus_tag="ACHKBL_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgdT"
                     /protein_id="HFO5818183.1"
                     /translation="MLSTESWDNCEKPSLLFPFTALTCDETPVFSGSVLNLVAHSVDK
                     YGIG"
     gene            22886..23575
                     /gene="mutH"
                     /locus_tag="ACHKBL_001291"
     CDS             22886..23575
                     /gene="mutH"
                     /locus_tag="ACHKBL_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HFO5818184.1"
                     /translation="MSQPRPLLSPPETEEQLLAQAQQLSGYTLGELAALAGLVTPENL
                     KRDKGWIGVLLEIWLGASAGSKPEQDFAALGVELKTIPVDSLGRPLETTFVCVAPLTG
                     NSGVTWETSHVRHKLKRVLWIPVEGERSIPLAKRRVGSPLLWSPNEEEDRQLREDWEE
                     LMDMIVLGQIERITARHGEYLQIRPKAANAKALTEAIGARGERILTLPRGFYLKKNFT
                     SALLARHFLIQ"
     gene            23644..24357
                     /gene="ygdQ"
                     /locus_tag="ACHKBL_001292"
     CDS             23644..24357
                     /gene="ygdQ"
                     /locus_tag="ACHKBL_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HFO5818185.1"
                     /translation="MLFAWITDPNAWLALGTLTLLEIVLGIDNIIFLSLVVAKLPTAQ
                     RAHARRLGLAGAMVMRLALLASIAWVTRLTNPLFTIFSQEISARDLILLLGGLFLIWK
                     ASKEIHESIEGEEEGLKTRVSSFLGAIVQIMLLDIIFSLDSVITAVGLSDHLFIMMAA
                     VVIAVGVMMFAARSIGDFVERHPSVKMLALSFLILVGFTLILESFDIHVPKGYIYFAM
                     FFSIAVESLNLIRNKKNPL"
     gene            24495..24713
                     /gene="ygdR"
                     /locus_tag="ACHKBL_001293"
     CDS             24495..24713
                     /gene="ygdR"
                     /locus_tag="ACHKBL_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YgdR"
                     /protein_id="HFO5818186.1"
                     /translation="MKKWAVIISAVGLAFAVSGCSSDYVMATKDGRMILTDGKPEIDD
                     DTGLVSYHDQQGNAMQINRDDVSQIIER"
     gene            24821..25861
                     /gene="tas"
                     /locus_tag="ACHKBL_001294"
     CDS             24821..25861
                     /gene="tas"
                     /locus_tag="ACHKBL_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HFO5818187.1"
                     /translation="MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGI
                     NLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPD
                     QALDRKNIREALHDSLKRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDT
                     LDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVG
                     LAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAA
                     YVDIARRHGLDPAQMALAFVRRQPFVASTLLGATTMEQLKTNVESLHLELSEDVLAEI
                     EAVHQVYTYPAP"
     gene            complement(25893..27086)
                     /gene="lplT"
                     /locus_tag="ACHKBL_001295"
     CDS             complement(25893..27086)
                     /gene="lplT"
                     /locus_tag="ACHKBL_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HFO5818188.1"
                     /translation="MSESVHTNTSLWSKGMKAVIVAQFLSAFGDNALLFATLALLKAQ
                     FYPEWSQPILQMVFVGAYILFAPFVGQVADSFAKGRVMMFANGLKLLGAASICFGINP
                     FLGYTLVGVGAAAYSPAKYGILGELTTGSKLVKANGLMEASTIAAILLGSVAGGVLAD
                     WHVLVALAACALAYGGAVVANIYIPKLAAARPGQSWNLISMTRSFLNACTSLWRNGET
                     RFSLVGTSLFWGAGVTLRFLLVLWVPVALGITDNATPTYLNAMVAIGIVVGAGAAAKL
                     VTLETVSRCMPAGILIGVVVLIFSLQHELLSAYALLMLIGVLGGFFVVPLNALLQERG
                     KKSVGAGNAIAVQNLGENSAMLLMLGIYSLAVMVGIPVVPIGIGFGALFALAITALWI
                     WQRRY"
     gene            complement(27079..29238)
                     /gene="aas"
                     /locus_tag="ACHKBL_001296"
     CDS             complement(27079..29238)
                     /gene="aas"
                     /locus_tag="ACHKBL_001296"
                     /EC_number="2.3.1.40"
                     /EC_number="6.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008779 -
                     acyl-[acyl-carrier-protein]-phospholipid O-acyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008922 - long-chain fatty acid
                     [acyl-carrier-protein] ligase activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
                     /protein_id="HFO5818189.1"
                     /translation="MLFSFFRNLCRVLYRVRVTGDTQALKGERVLITPNHVSFIDGIL
                     LALFLPVRPVFAVYTSISQQWYMRWLKSFIDFVPLDPTQPMAIKHLVRLVEQGRPVVI
                     FPEGRITTTGSLMKIYDGAGFVAAKSGATVIPVRIEGAELTHFSRLKGLVKRRLFPQI
                     TLHILPPTQVEMPDAPRARDRRKIAGEMLHQIMMEARMAVRPRETLYESLLSAMYRFG
                     AGKKCVEDVNFTPDSYRKLLTKTLFVGRILEKYSVEGERIGLMLPNAGISAAVIFGAI
                     ARRRIPAMMNYTAGVKGLTSAITAAEIKTIFTSRQFLDKGKLWHLPEQLTQVRWVYLE
                     DLKADVTTADKVWIFAHLLMPRLAQVKQQPEEEALILFTSGSEGHPKGVVHSHKSILA
                     NVEQIKTIADFTTNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYPSPLHYRIVPEL
                     VYDRSCTVLFGTSTFLGHYARFANPYDFYRLRYVVAGAEKLQESTKQLWQDKFGLRIL
                     EGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARLLSVPGIEEGGRLQLKGPNIMNG
                     YLRVEKPGVLEVPTAENVRGEMERGWYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMV
                     SLEMVEQLALGVSPDKVHATAIKSDASKGEALVLFTTDNELTRDKLQQYAREHGVPEL
                     AVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEAEQHDE"
BASE COUNT         7238 a         8032 c         7325 g         6930 t
ORIGIN      
        1 ctccgtgatt atttgcgaca actcaataat ccgtactgat tcataatcaa ctgaattttc
       61 ataattattt aaaatatgca atgctataaa accaatctca tcatcaggaa gttgaacgct
      121 gaattgctca ttaatgatag ttaatgcatc gcgacttatc gcatactctg ctttgtacaa
      181 cgctttgatt tcatcaagga aaacattatt aatttgtata cctgaaacta ctcgggatat
      241 tgcattcgaa atgtgatccg caagaatcaa tagtagcgat ggattgagat ttttttcgag
      301 atttttctcc gcatattgca ctattttttc ggcaacaaaa atatactcaa catccacatg
      361 ttcaatcaat ttgtagagtt tatttttttg ttcgtttctg acataaaaaa tccggttagc
      421 cggatgttca gggaccgaca ttccatattt tttgttgtaa ccgacgccag gtccagaaat
      481 gataacctcc tgtccattca tcgatgcctg tacacaatta ttgttcatga ctttttcgat
      541 gatcattcca tgactccaaa aaaaaaggca aggcacgtca gaagtaataa ttacttcaaa
      601 cattgccttg cctgatttaa cagttacaag atgattaact aatgcgtata cattacacgg
      661 aattgtgctt aattaaagag atggtcggct tatttttcaa gccgacattc aaatattcga
      721 catcagtcac aaaaccagca tctaatattc atgaaaatgt tatctattca acgaaatata
      781 aattcattga ataaataaaa acatcatccc cttctcgcca gcgtcgcccc atcggcaaaa
      841 tacgctttaa tccccgcaag aatagactcc gcaacttcct gctggaaagt cgccgttttc
      901 agtttacgtt cttcctcaac gttactgata aacgccgttt cgaccagaat ggagggaata
      961 tctggtgcct ttagtacggc aaacccggcc tgttcaactt gatttttatg cagcttgttg
     1021 attttaccga gcttattcag caccgcttta ccaaacttca ggctgtcggc aatggtcagc
     1081 gactgcacca tatcgaacat ggtgtggtcg acatagcggt caccgctttt gctcacgcca
     1141 ccaatcaagt ccgaggcgtt ctgggtttgt gccagatatt ttgccgcagt actggttgca
     1201 cctttggttg agagcgcaaa cacagaggaa ccgctcggct gacgactggt aaaggcgtcg
     1261 gcatggatag agacaaacag gtcagcacgc tgtttctggg cttttgctac gcgcacttgc
     1321 aacggaatga agatgtcttc attgcgcgtc atgtacacct tcatattgcc ctctttctcg
     1381 atcagagagc gcagacggcg agctatttgc aataccacgt ctttttcgcg cgttttgtat
     1441 ttccccaccg caccggagtc ttcgccaccg tggccagggt caagcataat gacaatagga
     1501 cgatcgcgcc ctgctttacc cggttgtgga ccactttgtg ctggcggcac ctgcttttcg
     1561 aggtcgcctt tgttgtaatc ctccagcagc gccagcagcg ggtcctgcat atcctgtgca
     1621 ttggccggat agaggtccat caccagacgc tctttaaacc cggcgaccgg cgcaagggca
     1681 aacagctgcg gttttacgtt ttgctttaat tcaaaaacca tacgtacggt ttgcgggtca
     1741 aattgcccga cgcgcgccga cttgatgaac gggtcgtcag cgcggatttg cgcagccatc
     1801 cccttgagta ccgagttcag gtttacatct tcgatatcca ccaccacgcg ttcaggatta
     1861 ctcaacgcga actgcttata tttcagctga cgatttgatt ctaccgtcac gcgggtgtag
     1921 ctggacgcag gccagacgcg caccgccacg acctggctga ccgcagccag gctgacctga
     1981 cttacgctca atagccacat ggcacccgcg ccttgcagta aacgacgacg gctgattgca
     2041 gtgttggatc ctgacatgcc tctcccgagc aaaagaaatc taagctgtgt aacaagtaaa
     2101 cgaccaattt gaccggaaac tttagcgaaa gacgcataat ctgtcatcta taaaacggta
     2161 aacattcttt ttatattcac ggtattactg ataaaaaact cgctcccgca taaaatttac
     2221 acttgcaccc tgcgaaaaaa cagaataaaa atacaataat ttcgaataat catgcaaaga
     2281 ggtgtaccgt ggtaaaggaa cgtaaaaccg agctggtcga gggattccgc cattcggttc
     2341 cctatatcaa tacccaccgg ggaaaaacat ttgtcatcat gctcggcggt gaagccattg
     2401 agcatgagaa tttctccagt atcgttaatg atatcgggtt gttgcacagc ctcggcatcc
     2461 gtctggtggt ggtctatggc gcacgtccgc agatcgacgc aaatctggct gcgcatcacc
     2521 acgaaccgct gtatcacaag aatatacgtg tgaccgacgc caaaacactg gaactggtga
     2581 agcaggctgc gggaacattg caactggata ttactgctcg cctgtcgatg agtctcaata
     2641 acacgccgct acagggcgcg catatcaacg tcgtcagtgg caattttatt atcgcccagc
     2701 cgctgggcgt cgatgacggc gtggattact gccatagcgg gcgtatccgg cggattgatg
     2761 aagacgcgat ccatcgtcaa ctggacagcg gtgcaatagt gctaatgggg ccggtcgctg
     2821 tttcagtcac tggcgagagc tttaatctga cctcggaaga gattgccact caactggcca
     2881 tcaaactgaa agctgaaaag atgattggtt tttgctcttc ccagggcgtc actaatgacg
     2941 acggtgatat tgtctccgaa cttttcccta acgaagcgca agcacgggta gaagcccagg
     3001 aagagaaagg tgattacaac tccggtacgg tgcgcttttt gcgtggcgca gtgaaagcct
     3061 gccgcagcgg cgtgcgtcgc tgtcatttaa tcagttatca ggaagatggc gcgctattgc
     3121 aagagttgtt ctcacgcgac ggtatcggta cgcagattgt gatggaaagc gccgagcaga
     3181 ttcgtcgcgc aacaatcaac gatattggcg gtattctgga gttgattcgc ccgctggagc
     3241 agcaaggtat tctggtacgc cgttctcgcg agcagctaga gatggaaatc gacaaattca
     3301 ccattattca gcgcgataac acgactattg cctgcgccgc gctctatccg ttcccggaag
     3361 agaagattgg ggaaatggcc tgtgtggcag ttcacccgga ttaccgcagc tcatcaaggg
     3421 gcgaggttct gctggaacgc attgccgctc aggcgaagca gagcggctta agcaaattgt
     3481 ttgtgctgac cacgcgcagt attcactggt tccaggaacg tggatttacc ccggtggata
     3541 ttgatttact ccccgagagc aaaaagcagt tgtacaacta ccagcgtaaa tccaaagtgt
     3601 tgatggcgga tttggggtaa gtggtggaaa tcgccagatg cgacatgcgc aatgcctgga
     3661 ggcatgataa acacgctagc atcgcatcag gtattgacgc tcatcgtcgg atgcggcgtg
     3721 aacgccttat ccgacctaca tcaaatcaaa ggtgttgatg gcggatttag ggtaagcgac
     3781 ggaaatcgcc agatgcgcat atgcgcaatg cccggaggca tgacaggacg cgcatgcgtc
     3841 gcatcaggca atcacgttta ttcccgcgaa ctaaacaatg ccgccagacc actgcgccgc
     3901 tcagtgcgcg tggcaattgc cgcacttaaa atgcgctcat cggcatacag cgacagacgg
     3961 cgacgcgcgc gggtcactgc ggtgtaaacc agctctcgcg ttactaccgg cgtgcgttga
     4021 ctcggtaaaa tcaacgccgc atggtcgaac tccgatccct gcgatttatg taccgtcatc
     4081 gcccacgtcg tttcgtgctc tggcaggcga ctcggttgca cagacttaat attgccgtcc
     4141 ggcatcgcaa accaaacgcg cgtcccctgc ccgcgatcca gcgcaatacc gatatcgcca
     4201 ttaaacaacc caagcgcgct gtcattacgg gcaatcatca ccggtcggcc ttcgtaccaa
     4261 cgagagtgcg gattacgatg aattttgcgc ttctgttgca taaactgctc aattcgctca
     4321 ttcagtccag ccacgccaaa cggcccttcc cgcagggcgc acaaaagctg gtactcattg
     4381 aacgcctgaa tgattaaatc cggctcggca cgcgcttgca gcagatccag ataacgcccg
     4441 taacccgcaa gagcctcctc aagcatcgca atatagtctt cgccgctctg taagagccgt
     4501 ttttcgatat cagtaaaatc ctgctgaaaa acggttttca ctgccgtttt atcaccacgg
     4561 ttgatcgccg cagctaactg accaatgcca gaatcgctgc cgaaacgata gcttttttgc
     4621 agcaggcaga gactgtcgcg caaagatgcc gcttctgtgc cagttcctga cggaacgtga
     4681 gttcccgtca ggcggctcaa ctgcccggca cgctcggcgg taaagcccgc gttggcataa
     4741 gcgcagatat cgcccagcac agctccagcc tcaactgagg ccagttgatc gcgatcaccg
     4801 aggaagatca ctcgcgcatg atcgggcaac gcatcgatca ggcgcgacat catggggaga
     4861 tcgatcattg acgcttcatc caccaccagc acatcaagat gcagcgggtt accggcatga
     4921 tgacgtaaac gctggctacc cggctgcgca cccagcaatc ggtgcaaagt gctggcatct
     4981 tccggaatgc gtttcttttg ttcatcggtc agcggtaact ggcgcaaagc cttgccgaga
     5041 gattcggtta agcgcgcggc agctttaccc gttggtgcag ccagacggat acggcagcgt
     5101 tcgccgtctg ccatttgaat taacgctgcc agcaacttcg ctacggtggt cgttttaccg
     5161 gtgccagggc cgccggaaat caccgagatc cgccgcgtca gcgccactgc cgccgccact
     5221 ttttgccagt taatttcatc gcttactgga aaaagtttgt ccagggtttg cgccagtaga
     5281 gcttcatcaa cctcaatggc atgattcact tcgttgaaaa agcgtgccac tgtgcgctcg
     5341 ttacaccaca tgcgattcaa ataaagacga tcaccacaga ggatcatcgg cgttggttca
     5401 tctccccggc tgaccgcttg tgaagccagc aagcattctt cccaattttg taactcaccg
     5461 atttcgctga cgcaggttgc taacagcgga tgtgacgcct cgttattttc cagtcgtgag
     5521 agcggtaaac agacatgccc ttccccggcg tcgtggctta acagggctgc cgcgagggtg
     5581 acggcaggat gttcatctcc cgccacggtc agggcaaatt gcacatccag cgggcgtaac
     5641 tgtttgtgct ccacagcttc cagtaattgc ttttgcaatt tcattacgcc tcctccaggg
     5701 tgataccggc aaacatctca tccatcaggt caatcaaccc ggcgttgggt cgggtcgcgt
     5761 agatcccttg ttgcggatgt tctttatcaa caccacgcag gaacagataa ataacgccgc
     5821 caaagtggcg ctcatagtcg taatcagcaa tgcgatggcg cagataacgg tgcagcgcca
     5881 gggtataaag ctgatattgc agatcatagc ggtgtgcctg cattgccgct gccatagcct
     5941 gttgggtgta agcagaactg tcttcaccca accagttgga tttatagtcg agcaggtaat
     6001 aacgcccttc gtggcggaac accaggtcaa taaagccttt taacatgccg cgtacctgca
     6061 tgaactccag cggcgggcag ccagcggata gcgagtcaaa ctggcgaatt agtgcatcaa
     6121 gctggctggc gatgagcggt tcgctaatcg gcaggtaaaa ctccatctcc acctgtttat
     6181 cgcggtcgga aagctgactc aggctaacac ccgtttcatt gagaggtgcc tggaggacag
     6241 ccgtgatcca ctcggtcaac accggttccc actgcgattc aaagccgccg agttccagtt
     6301 tttcctgtac ccagttcggg tcaaccggct gggtaaaatc gaggtcttca aacaaactgt
     6361 gcaagaatgt ccccggtgac gcaccgcgcg ggaactgatg cggtgttaac gtcggttcgt
     6421 caacgacgct gaccacgcct gcggcatcaa catccagccg tggcatcaga tcctgagcga
     6481 taccgtgacc acgctgctgc aaaccggagt agctggtgac acgccagtta tcgccgggca
     6541 atcgttgtag cgtcctcgca ttcagttctg cagttatagc atcattaacc tgccagggct
     6601 ggttatcacc agtttgtgcc gtttgccagg caatatcatc atcgcataac gcttcaatac
     6661 aggtgcgaag ccctgccgca tcttgcggtt cccctttttg cagcaaacgc ccgagcgcac
     6721 tttggtggac gtcggtgtca ccttttttat cgccacgacg gcgcaccagc ggtgcaacgc
     6781 cgagactgca atgccaaacc gaacgcgtca gcgccacgta aagcaaacgc agatcttccg
     6841 ccagacgttc ggcctccgcg agatcgacgc tttctggcgc agcattaaga tccagaacag
     6901 cctcaaacga gtggcgatcg tgatagaacg cctgatcctg gacgcggaaa ttggtaataa
     6961 atggcaacca gaccagcgga tattccagcc cttttgattt atggatcgtg acaatctgca
     7021 ccagatgttt atcactttcg aggcgcattt gttggctgga ggcattactg tctggctcga
     7081 ggatatgttg cgataaccag cgtaccagcg catgttcact ttccagctgc gttccggctt
     7141 cttgtagcaa ttcgctgata tgcaagatat cggtaagacg ccgctcaccg cctgctgttg
     7201 ccagcaagtt ttctgcaatg ttacgcgccg acatcagcgc ccgcagcatc ggcataacgc
     7261 cacgtttgcg ccagatttgc cgataaccat cgaactcttc gacgaccgca tcccacgcat
     7321 gttcgtcatt attcagcgtt tcaatatcca gcgcattcag ccccatcatt gacgttgcca
     7381 gcgcactgcg cagggtgttc tcacgttcgg gagtcatcac cgcctgcaac agccaaagca
     7441 tctcctgcgc ttccagagtt tcaaaaacac tgtcgcggtt cgaaaggtaa acggaaggga
     7501 tttccagcag cattaaggca tcgcgcacct gggcggcctc ctggcggctg cgcaccagca
     7561 cactgatgtc cgaagcacgc accggacgcg cgtcgtcgcc gttcatcagc aacgcttcgc
     7621 cccgctgtcc ggcttgtagc cagtcgcgga tttgcgcagc acatacctgc gccatggtac
     7681 tttgatagtc gccaacgccg cagctttcgc cttccatcag ccacattttc atcgcaggct
     7741 gcgtttcacc tttaaacaca aaacgtaacg cctgattttt gccggcagat ttcacaggaa
     7801 taaacggtat ttcacgaaac atgaacgcgt catcagtctg gctgaaaagt ttgttaacgc
     7861 tgttcaccat tcccggtgcg gaacgccagt tggtatctaa ggtgtagtga gcatgaactt
     7921 cgctacgcgc cttcatataa gtgaagatat ccgcaccccg gaatgcataa atagcctgtt
     7981 tcgggtcgcc aatcagcaac aatgcggttt ccggctgatg gtgccagata cggcgaaaaa
     8041 ttcgatactg ctgggggtcg gtatcctgaa attcatcgat cattgccacc gggaatcgcg
     8101 tacggatcgc cgctgccaat acctcgccgc tttcactacg cagcgcggaa tcgagccgac
     8161 ttaacatgtc atcaaaaccc aattcgccac ggcggcgttt ttcacgcgct actgtttcgc
     8221 ggatctcagc caatgcgcgg gtgatcacca gatcgcggat cgacaatggt tctgcaagca
     8281 gttgatcgat cgcctcaaac agtggatgtc gcggggtttc ccccccggct ttcgtgcgat
     8341 cttctaagaa acgctgagag aatttttcca gcgactccgg caactgatag ctgttggtct
     8401 cttcttctgc ccaggcgctg atcttgtcga tccatttagc ctgattgcta cggttaaact
     8461 tgcgtcgatc aataccagaa gattcgatca gcgcatccag ttcacccact gcgtcgcgcc
     8521 actgctgttt tacggcatca atacgcgcca caatttgcgc gtggcgggaa gcaagcgttt
     8581 catcatcggg cggcggtgct ttgataaccg gtgcttcgcc ttgcagataa cgattaatat
     8641 cgcgcagcaa cgcctgcggc cctttccagg tttcaaagac cacctgggca atttcacgcg
     8701 gcagcgggta gcagtggcga cgccagaaat cggcgcaggc ctggtagcgt agcagagact
     8761 catcttcaat cagctgctgc tcaaacagca tgccggattc aaaggcattc aggttgagca
     8821 tgcgctggca aaaaccgtga atagtaaaga ctgccgcttc atccatctgc cgttcggcta
     8881 acaacaacca ctgcgcggct tgcgctttat cgtcgatctc ctccagcagg agtttgtaca
     8941 gtggattgtc ggtggtttca cgcagacagg cgatgcgcaa ctcgtggata ttgctacgga
     9001 tacgaccgcg caattctgcc gtggcagcct cggtaaaggt gaccaccagc agttcttcaa
     9061 cggtcagcgg gcggggaaag gcggcagaac cgcctagtcc aagtaacagg cgcaaataga
     9121 gcgccgcaat cgtaaaggtt ttgcctgtgc cggcagaggc ttcaatcagg cgctcgccct
     9181 gtaagggcaa gcgcaaagga tctagtgtct cggcgacatc actcattctt ttcactcatc
     9241 aggggcattg tttgctgcaa cgcgctgacg ttctcccaca ctttccagcc ttcaggatgc
     9301 acatattcgg ctttcccgtt ctggctgccg gaaatctgcg acagaatagc catgccttgc
     9361 ggctcgacca ccgcctgatg gaagaaatca gcaagttttt gcggcgtcag cagttttatc
     9421 tgggccacga ttttatcacg cgaatcgaag cgcatattgc cgcgatcgaa atctttactt
     9481 aacttcgatg cttcttcgcc gagcgtttgc ggtgcctgca gcatctgggt tattaccgcc
     9541 tgctggattt gcgcaaactc ctctggcttc atcgcccgca attttgcctc tgcggttggg
     9601 aaaaacgcct tgtaacgctc ccacaagaat gaaggctgtt tatcattgct ttgcaaaagg
     9661 aagcccatgc cccactgacg ccccacgctc attggaaacg caaacacggc atagcccaac
     9721 tgttcttcgg tacgcaactg attgtagaac cacggctgta cgatctgccc caacagagag
     9781 ctataggctg agctggtgta ttcatcgtag ccagtcggta caaataccgc tgccagtgcg
     9841 gagtcggtgc tgttaccggc tttttcaaag atgacagatt gttttttatc gaccacgaca
     9901 tctttgttac ggcaccactc cgaaccatca gcgcccaact gtttttgcac atcgcgtgcc
     9961 agcgttgttg cctgggcttc ggtcatgttg ccaataacca taaactctgg tcgagcccct
    10021 gattttaagg cgtcgcgata ggccagcacc tctttcaaag tgattgaagg caaaattttg
    10081 cgccgttcat ctcgcgagaa gtacggcact tgcgagagca tctgcgcggg cataatcgcc
    10141 tgctcgaacg ctttgccctt ttctgcggaa tccatcatct ggttatacca ggacttcgcc
    10201 tgctcaagct gatcttccgt agcggtatag ctaaagtacc cctcgagcaa tgcctggaac
    10261 agctgcggca gacgctgggt gtaaccatta gcattaacca taaggccgtt gttagcgttg
    10321 gtggaaaaac taatgccacc aaccgacgcc tggttgctta actgatcaag cgctagcccg
    10381 gcaagataat cattgagcgc aaacatcacc tgattgcggg cgctgtccat ggctttcgga
    10441 ttacgcaaaa tcaggctgac atcagctttg ggttcgctgg aaaaataacg gcttggcgca
    10501 tagaccacac gcagattcga ctcatcaaca ataagctctg gatggtcata tttcttctct
    10561 gacttaatca gcgagaaatc gtcaggaata taggggttaa gctctggcaa agagagcgca
    10621 atgtcggcgg cttttttctg ccagtcagcg aaagtttgtg cgctaatttt atcgacctga
    10681 tacggcgcat cgacaaagta agccgttttg ttgtgcggct ctttcgggct gatataccag
    10741 atacgcgcat tctgcggcgt catcatcgcc aggcgttctt ttactgcttt agcgtcgtac
    10801 cgatcggcaa tattgactgc atccagcgta tgctcaacag gaacgcgaat catggtatct
    10861 gccagccatt cgacgtaatc catatcacgg gtgattgacg gataacgaaa gtcgatatcc
    10921 agcacattcg ccagttcatc gaagtattgt ttatcaatcc ctttttcgcg taacagattg
    10981 aggtagctaa aaatggccgc cacaacctga tcgcgattcg ccagaccttt atcggttaaa
    11041 gacgcagaga tcgctaatac gccgctgttg ccgttgacga taggatcgga attggcgcta
    11101 atgccctcaa ctaatccctg cttttgcagc cagtcagaaa gtgtacctgg gctgcgatta
    11161 ccgatcagat aggtaatcaa ttcatccgtt ttactacgga acttcgctga attgttatcg
    11221 atgcgaaact caacgcgcag aactttacgc ggcagcgccg ggacgtaatg aatgataata
    11281 cctttttgcg cgtcggtgac taccggcacg gtgatttccg gttttttgct ctctttgttc
    11341 ggcacgcgac caaaggtgtc cgccgccatt tttgccaact ccggcagcgg tttattgctg
    11401 taaataaccg ccttcatcag attggcagag taatatttct cgtgaaaatc tttcagcgcc
    11461 tgctgcaccg gatttccggg tttgtcgctt aaagtttcga ggttaccacc agaaaacttt
    11521 gaaccggggt gtgccgggtt aatggtttct gcgctgacct gtgccatgcg catcccgtca
    11581 cgcgtacgcg ccatggttaa ttcggcattc actgcattac gttcacgttc ggcgtatttc
    11641 ttgtcgagca agggttctgc aatagcgtcc gccaggcggt ctaccgcacc gggcaaggcg
    11701 tcgttctcaa cttccagata gaaagccgtg cgatacggtg ccgtgctggc attgtgactg
    11761 ccgccgtgca ttttgagata ttcggccaga ctgtcagcct gcgggtactt tttcgacccc
    11821 atcagactca tatgttcaag gtaatgtgcc agcccctggt acgcctcggg atcttccagc
    11881 gacccaacgg gcactaccag cgccgagagc gatttaaccg cctgcggatc agaaaccagc
    11941 aagaccacca taccgttatc cagacgtata gcctgatact ggcggttatc tttatcactt
    12001 ttacggatgg tttcctgaat cggctgccat cccgtttctg cctgacttaa gggtgcccaa
    12061 agggcaacaa acaacaataa tgctttgaac caggtgctgc ggggcattca cggacctcat
    12121 aatcaacttt attttctggc cagattcaac aggttaacgc aaatcatctg ccagaattta
    12181 atcttgtgct gaacgagtca gcctatgtct atatatccat cagtccgtga ctggtgcgca
    12241 tcataaagca agcagatata ttgcgcaatt tttatacagc actcatgact gattaaagcg
    12301 aaacagcggt aacaggaaac gttgcgactg ttcaacgata gcctccattg tctctggtgt
    12361 taattgccgc cagagccgtt gataccagat atcatcacct tcgccacgca ccatcatgtt
    12421 gccttcgtaa gcctgaagga atttcgtacg ggctttttgt aacgtggaat cgtcatccag
    12481 catggcatcg ttttgcgcgt cataacaggt ttttagccac gcgccgccac tttcaggtaa
    12541 caccagcaat ggcgcggaca ttccttcacg atacccctca atcagttgtg agaggtaatg
    12601 caaagcctgt tcggctgcaa gcggcggaaa acgccactcg ccgtctttgc gtagaaaaag
    12661 gcgactttca ccattaccac cgctggcaca gtagacaagg tgttccagcc aaagttgcat
    12721 tccctgcgcc acacttaata aagagggacg ccagcgcaac aggccatccg gctgcacctg
    12781 cggcaaccag ccagttatct gcacaccgtt gcaggcgaga tcaatttcca tactctgccc
    12841 cggctggcga cgtgcaatga ctctgtcggc aagctgctgc atctcctggc actgtgtttc
    12901 ccagaaaatt tcaccaaaag cgccatacgg taaatcccct gccgcccgga agcggcggaa
    12961 taagcgatcg gcatcatcct gctcaaccag tgcattcaat aactgctgat tgatttgata
    13021 acggctaagc ccttccagaa taaatggctc ggtgtcgggg atttcgctgt cttcagtacg
    13081 gaagttcacc tgcaaacgca tctggaagaa cgcccgtacc ggatgtgccc agaatcgttg
    13141 tagcgtttcc agcggcacgg tttccggtaa ggtaaacggc agcggctgaa caaattcaga
    13201 atgtgcttta ccagcctggc tggccgcagg tagccattca cgagcatagc tttgtcgttc
    13261 gcctggctgg tagttttgcg gatcaaacgg catccgggta tggaggcaag taagatgcgc
    13321 ttttaccctt gcctcgcttt catcacagtt gagcgcttca tcgcccggta gataatggct
    13381 ttgcccgatg tagtcgatca gttcctgcac cagtaccgac gggaaacgtt cactgttatc
    13441 ctgaatagaa cgcccgatat agctgatata gagtttttgc tgcgcggaaa ttaacgcttc
    13501 caggaacaga tagcggtcgt catcgcgacg gctacggtcg ccacgcttcg gtttctggct
    13561 catcaggtca aagcccaatg gcgcaagctg gcgtggataa acgccgtcgt tcattcccag
    13621 caggcaaacc actttgaatg ggatggaacg cattggcatc agagtacaaa tgttaaccgg
    13681 tccggcgaga aaacgctggc tgatacgttc ctgatccagg cgctgtgcca gttcatcacg
    13741 caatagtgac agcggcaccg cgtcgccata ctgcgcacct aaaccttcgg cgataatcgc
    13801 ctgccattgt tgttcgatca gcgtcatagc cgcttcggtt tccgcatccg gcaggaagaa
    13861 ggcgttgagc atatcgcgac aaaccggcaa ccactcttcc agcggacgct cctgcgccag
    13921 cccgcgacgc cagatgttta gctgcattag cagtgaagcc agatgcccca ccagttctgc
    13981 aattaagccg ctcgattcat cataaggtag aaccgattgc cactcgccct gcgcgctctc
    14041 catcgcgtag cccagcaaca tacgcgtcag gccaaatcgc caggtgtgtt gtccggtggc
    14101 ggggagttcc agctcgcgaa cgttgtcgtc atctatcccc caacgaatgc cggattcgtt
    14161 gacccactgg cgtaaataac gcagcccttc ttcggtgatg tcaaaccgcg ccgccagcac
    14221 cggcacatcc agcagcgcca gcacatcctc tgacacaaag cgactgtcag gcagtgataa
    14281 caggctgata aacgcttcca ataccggatg tgactgccgc gcccgacggt cggaaatggc
    14341 gtaaggcagg taacgatccg caggtgcgct accaaacaca gcctgaataa acggactgta
    14401 gctgtcgatg tcggccacca tcacgatgat gtcgcgcgga gtcagtgtcg ggtcttcctc
    14461 cagcatcgcc agcaggcgat cgtgtaaaac ttcaacttca cgctgcgggc tatggcaaac
    14521 gtgaaaggtg atactgctat ccagtggatc aagcgggcgt ttgttatcgc tacgggaaaa
    14581 ctcttcgatg tttacaccag caacggcgcg gttttccagt tccagaatgt cagactgaat
    14641 gttatgcagc aggttatctg gcgtaacatc gacaaaagcg tccagctcct ggctgctctc
    14701 cagatcagaa aggagataaa tgtagtcgcg cccaagcttg ccccatgagg ccagcagcgg
    14761 gttgccgaca tcctgttcac catcgctgtt aaagagctgc ccggcatttt cgctgtcgcg
    14821 aaatagcggt aattctcgat cttcaaaact gtgtcggcgc tggcgagtca gtagtttcgc
    14881 cagataagca ggatctttaa tgtcgcccca gtaataacgg caggggttgg taaacaggag
    14941 atggatttca atatgtttac ccagcgcctg gagtgcctgg agataaacag gcggtaacgc
    15001 ggaaataccg catataaaga cgcgcgaagg taaccccggc gggcaggtcg tcgcggactc
    15061 cagcgtttcg ataaagcgct gatagagatt ggcgcggtgc cagcgcggtt gcccgagttc
    15121 atgggtatat tccaccaacg ccttccacaa cggcgcttgc caggcctgtg cttctcccag
    15181 cccttcaacc aaatgtccta tttcccactg tgccagccag tccggacgat agaccagata
    15241 ctggtcaaac aggtccgccg cttttgaaga aagctggaac agttttcgct tgtcgctatc
    15301 gtcagtcaga taatgccgca acagggtaaa gtcttcgcgc tccaacagtt gcggcagcag
    15361 agtcatcagt ttccagctca tgctctgttt gttaaaggcg ctctctttgg ggatttccgg
    15421 taacacccgg acgaacatat cccagataaa gctcgctggc agcggaaaat caatgtttgc
    15481 cgcaatacca aacttttgcg acagggtcat ttgcagccac tgcgccatac cggtactttg
    15541 caccagaatc atttctggtt cgaaaggatc gtccagccgt tcgcgttcga cgataaactc
    15601 catcaacgct tccagcacgt ccagacgatt ggaatggtag acccttaaca tagcggctcc
    15661 tgactactga cgattcgggc aatgcaggcg ggtcatctcg ccttctctgc ccccgggtga
    15721 aactagcgta acgctgatgc tgacacatcc cgcctgcgat gtctgcattc ggttgacctg
    15781 ccagttagca ggtggcgaaa tcgcgcgcag ttgcgtttgc tgccagccat gccgccagag
    15841 ctgttggtac tggtttctgc tggcaagact gttcattaat gttcgctgga aacccgataa
    15901 cgcagtgaca atcatcacca tcaacaccat cgccaacatt acctccggca gactaaagcc
    15961 ttgttgattc ctcagggaag ctgacataac gccctctctt tcagcggaca aaaatcgctc
    16021 cagccgcgtg gcgaaaaaac aatgtttcca tcgatgactt cgcctgttcg ccacaacgaa
    16081 acgccttcat aaccagcaat caataaggct tcattatctg ccagtaaacg cagacaaacc
    16141 cgggcaccgg tggcagcata cagtaagcac tgaactgcgg gcagcgtctg ccaggagtgc
    16201 atttttcccc actccagcgc cgactgaacg atggcctggc ggcgcaatga ctgactttcc
    16261 atgctcacgc gagaagcaaa actgcgatcc tgctgactca ttccttgcaa tagcaagcta
    16321 cccaaaatca gcagcatcag gaccagagcc agtgacgaaa cacccttttc gcggttcaca
    16381 ggttggatcc tgtcacgcta tagctggcat ccaccacggt ttgcggatca gccttgctgg
    16441 cagcgtgcat attaaccgtc aacaccggcg agaagccgct gacatcctga cgtacgacct
    16501 gaaaagtgtc gataatgatg gcatccggat tagtgacttt atcccagccc ttaccttcac
    16561 aggatgtcgc accgcgtagc gtttccagca catgctcctt cagacgaaat ccaatctggt
    16621 cggactcttt taccggttcg cgatcccaga taccattact gttcgcatcc cactgcacaa
    16681 tgacacagtc accctgtccg acaatttcca gcccttcgcc cgtacagctg ccatggcaat
    16741 aacccgccct ctggagatgc ttcgcgacgg taaatacccg cagccagatt tcatcttcca
    16801 gcgccagctt acgggtgttc gttaaacttt cacgctgtaa cgcaggcaga aagcgtgcag
    16861 cccccagcaa caatacgcta ctgatcgcca tagcaatcaa cacttccagc agagaaaaac
    16921 cttgctcttt tacaggcatc cttctgtttc tccttgctga caaagccgga gtcttcccca
    16981 cggcgaaacc actagccacc actcgcccgt tgagtttttg aagcgaatat gcccggccca
    17041 tgcggtattg cgcaggccaa agaaagcaag cgaaggtgtc aggtcgctca tttcgacttc
    17101 gggccagcgc ggcacaaaga ccaatggtga actgccatga caggtattcg ccccagcaac
    17161 ggaactcaca aggcaccata acgtcccctc cctgataaga ctgatactgt ggtcgcggtt
    17221 atgccagttg gcatcttcac gtaaatagag caaatagtcc cgcgcctggc tggcggtttg
    17281 ccaaagccgt tgcgactgct gccagtattg ccagccataa agcccacttg cgcttagcat
    17341 gaccagaatc agcatcgcga ccagcgtttc aatcagcgta taaccacgtt gtgttttcat
    17401 gccggcagta tggagcgagg agaaaaaaag acgagggcca gtttctattt cttcggcaca
    17461 tcttccggac tatttacgcc gttgcaggac gttgcaaaat ttcgggaagg tgtctcgaag
    17521 aatttaacgg agggcaaaaa aaaccgacgc acactggcgt cggctctggc aggatgtttc
    17581 gtaattagat agccaccggc gctttaatgc ccggatgtgg atcgtatcct tcaatctcaa
    17641 agtcttcgaa acggtagtcg aagatggatt cgggtttacg tttgataatc aacttcggca
    17701 gcggacgcgg ttcgcggctt aattgcagat gagtttgatc catatggttg ctgtagagat
    17761 gcgtgtcgcc accggtccag acaaaatcac ccacttccag atcgcactgc tgcgccatca
    17821 tatgcaccag taacgcgtag ctggcaatgt tgaacggcag gccgaggaag acgtcacagg
    17881 agcgctgata aagctggcaa gagagtttgc cgtctgccac atagaactgg aagaatgcat
    17941 ggcacggtgc cagcgccatt ttatccagtt cgcctacgtt ccacgctgaa acaataatgc
    18001 ggcgggaatc cgggtcgttt ttcagctggt tcagtacagt agtgatctgg tcaatatgac
    18061 gaccatccgg cgttggccag gcgcgccact gtttaccata cactggcccg aggtcgccgt
    18121 tttcatcggc ccattcgtcc cagatggtga cattgttttc gtgtagataa gcaatgttag
    18181 tgtcgccctg caggaaccac agcagttcgt ggatgatgga acgcaggtgg caacgtttag
    18241 ttgtcaccag cgggaatcca tcctgcaggt taaaacgcat ctgatgacca aaaatggaaa
    18301 gcgttccggt tccggtacgg tcgtttttct gtgtgccttc gtcgagcact ttttgcatca
    18361 gttctaaata ctgtttcatg gttcctcagg aaacgtgttg ctgtgggctg cgacgatatg
    18421 cccagaccat catgatcaca cccgcgacaa tcatcgggat ggaaagaatt tgccccatgc
    18481 tgatgtactg cacccaggca ccagtaaact gcgcgtcggg ctggcggaaa aactcaacaa
    18541 tgatgcgaaa cgcgccgtaa ccaatcagga acaagcctga gacagctccc attgggcgcg
    18601 gtttacgaat atacaggttg aggataataa acagcaccac accttccagc agcagctcgt
    18661 aaagctgtga tgggtggcgc ggcagcacac cgtaagtgtc gaaaatggat tgccactgcg
    18721 gattggtttg cagcagcaaa atatcttctg tacgggagcc agggaacagc atggcaaacg
    18781 ggaagttcgg gtcaacgcgg ccccacaatt caccgttaat aaagttgccc aggcgcccgg
    18841 caccaagacc aaacggaatg agtggggcaa taaaatcaga gacctggaag aaggaacgtt
    18901 tagtacggcg ggcgaagata atcatcacca cgataacgcc aatcaggcct ccgtggaagg
    18961 acatgccgcc gtcccataca cggaaaagat acagcggatc ggccataaac tgcgggaaat
    19021 tgtagaacag aacataacca atacgtccac cgaggaagac gccgaggaag cccgcataga
    19081 gtaagttttc aacttcattt ttggtccagc cactgcccgg acgattcgcc cgtcgtgttg
    19141 ccagccacat tgcaaaaatg aaacccacca gatacatcag gccgtaccag tgaagcgcca
    19201 cgggtcctat tgagaaaatg accggatcaa actccggaaa atgcagatag ctactggtca
    19261 tctgtcacca caagttcttg ttatttcgct gaaagagaac agcgattgaa atgcacgccg
    19321 caggtttcag gcgctccaaa ggtgcgaata atagcacaag gggacctggc tggttgccgg
    19381 ataccgttaa aaaatatgta tatgatccgc gctataaccc cccgcgaatc aacccgccca
    19441 tgccgcgacg ctccataaag gctgcaacct gatggcgaac ttcggtcgcc agttgcgctt
    19501 ccagactacg ctgcgcaaga ttttctgctt cagcaaaatc aatgcgccgc agcaggtatt
    19561 ttacgcgcgc tacagaacgt ccgttcatag acagatggcg atacccaagc ccaatgagga
    19621 ttgccacgca catgggatcg cccgccattt caccgcacaa acggagatcg attccatgta
    19681 tttccgcttc ccgggcgatc atcgccagag ctcgtaacat tgcaggatga agactgtcat
    19741 aaatgttcgc cacccgggta ttgttgcgat caacggccag gatgtattga gtcagatcgt
    19801 tggtgccaac agagatgaaa tcgacccgct ttgccagatg cggcagcata aataccattg
    19861 acggcacttc cagcatgatg ccgatacgtg gtttgggaat ttcgtaaccg atcatctcct
    19921 cgacttcacg tccggcacgt tcaatcaggc ggcgtgcttc gtcaacttca tcgaggcttg
    19981 tgaccatcgg caacagaata ttcaggttgc ccgtagcggc attagcacgc agcatcgccc
    20041 gcacctggat caagaagatc tccggctgat cgagcgtaat gcgaatccca cgccaaccca
    20101 ggcatggatt ctcttcgcta atcggcatgt aaggcagctg cttatctgct ccgacatcca
    20161 gcgtacgcaa ggtgacgggt ttatcattaa acatttgcag catcccctga tactgcgcca
    20221 cctgttcttc ttccgacgga aaaccacttt gcagcatgaa tgggatttca gtgcgataaa
    20281 gtcctatgcc atcaatacgg ctgcccagtt tttcttcatg ttccgggctt aaaccggcat
    20341 tgagcatgac tttaatgcgc tcgccgcttt ttaactgggc gggtaaattg acgtcatctt
    20401 ccgccagacg gctaagctcg atctcttcac taattagccg ctgatattct tgcagcagta
    20461 ccggttccgg atcgaccagc aattcaccgc gataaccatc gacgatcagc gtccgacgat
    20521 gcagtaccga aggctgaata tccgcgccca tcacggtagg tatccccagc gcacgtacca
    20581 tgatcgcagc atgggagttg gcagcgccat cgcgcacgac aacaccgact aagcgatcct
    20641 ggggcagctc agcaagcgtt gtcgctgaca gttcatctgc caccagaatg aaacgttccg
    20701 gccaggcgtt cggcccttga ttagcgtcat caagatgaaa cagcaatcgc tgacccagcg
    20761 cacgtaaatc gccagcccgc tctttgagat agttatcgct tagcgcggca aactgttcgg
    20821 caaatttttc aatgaccgtt tttaccgccc actctgccac cgagccttta tcaacctcgg
    20881 caaacaattc gcgacgcagc cgggtatccg aaagcaggtg tgagtaaaga tcgaaaatag
    20941 ccgccgtttc tttttgtgca ccggcagcaa agcgtttgct atagcggcga aactcgttag
    21001 ccgcctcttc caacgccccg gtcagtcgtt cgcgttccag agccggatcc agcgttgatg
    21061 cctgatacac ctgttccatt aaaggtaacg tggcatcctg ccagccttcg gcaatcgcca
    21121 caccaggtgc tgccggtaat gcacggattc gcgtctggcg atattgccca aacaaggcag
    21181 tcaactgcga ctgagaaaga atagctgcca tctgggtggc aagcgtcacc aggaaggatt
    21241 cttcactttc gtcatactgg cgcaactctc gttgctgtac caccagtaca ccaagcaact
    21301 ggcgacgttg aataattggt acgcctaaaa acgcgcggaa acgttcttct tttacggagg
    21361 ggatgtattt gaagctgggg tgcttttgcg catctgcaag gtttatcggt tccgccagcc
    21421 tgccaaccag gccgacgatc ccttcatcaa acgcgagcgt tacagtgcga ccgcgtggtt
    21481 ttttcagtcc ccgggtcgcc atcaggtagt aacaacgtcg atcatgatcg gccaggtaga
    21541 ccgaacagac ctcggtatcc atcgcaagac agatgtcggt aaccagaata tttaacgcct
    21601 cattcaggcg tggtgcgctg gctacctttt cgactatttc gcgcaggcga gtgagcataa
    21661 ttggcgtgac ttaacctctt ttacgtcgat aagcagatgc gttttgtggt tttggcgtat
    21721 tttcctgcag tgacatcacc acactcgcga actctttcat taccctacgg tagacatcac
    21781 gtttaaatga caccacctgt ctgactggat accagtaact tacccatcgc cagccgtcaa
    21841 actctggtgt actgctggtt tgcatattga tttctgcatc gccgctcacc agctgcaaga
    21901 gaaaccattt ttgtttttgg ccgatacaaa ccggcttcgt gtcccaacgc accaaacgtt
    21961 tcggtaattt gtagcgcaac cagttacgcg ttgaagcaag gattcgaacg tctttgcggc
    22021 ttaatcctac ttcttcaaac aattcacggt acatcgcctg ctctgcggat tctccggggt
    22081 tgattccgcc ttgcggaaat tgccaggagt gctgaccaaa tcgccgggcc cacattacct
    22141 gcccctggcg attacaaatc acgataccta cgtttgggcg gtagccatcg tcatcaatca
    22201 ccggactacc tcaaaataaa gctttatata cgaatgattg tttcatactc caggaagacg
    22261 gtaaaccact ctctgcaggg cattacacac taataacaat tgaataactc acagttatgt
    22321 gcagagttat aaacagagga aggggtggat agccgttttt attcaccttt tctgtggata
    22381 gagttgtgaa gaactacgga attactacgg gaaaacccgg agcattctga ataagacaga
    22441 tacagagatt ttaaaatatt cattatcagt caatgcatta catgtgattt gagtaataaa
    22501 aaagagtaat tcgtgaccca ggtcacacct ctcatttacg ggttggcgaa agatcaacca
    22561 atgccgtatt tatccacaga atgtgccact aagttaagca ctgaaccact aaaaactgga
    22621 gtttcgtcgc acgtcaaggc tgtaaatgga aacagtagtg aaggtttttc acagttatcc
    22681 cagctttctg tggataacat ggtgtaagat cctgtttatt ttcagtgacc agatttggaa
    22741 aacccgttgc agtgttgcgc aactcgatta ccggcaacct aaaaagcaat ataaatcaga
    22801 gaattgaaca acgcatgtgg aaaaagttac actgcgaata ttcggcactt aattgctgtt
    22861 tgttttttaa ccaaggtatc atgacatgtc ccaacctcgc ccactgctct ctcctcccga
    22921 aactgaagaa caattgttag cgcaagcaca acaactttct ggttatacat tgggagaact
    22981 ggcggcactt gccgggctgg ttacgccgga gaatttaaaa cgcgataagg gctggattgg
    23041 cgtgttactg gagatctggc taggtgccag cgcagggagt aaacctgagc aagattttgc
    23101 tgctctgggc gtggaactta aaactatccc tgtggatagt cttggtcgtc cgctggaaac
    23161 aacattcgtt tgtgttgccc cgttaacggg caatagcggg gtgacctggg aaaccagcca
    23221 cgtgcgccac aagctcaaac gcgtgctgtg gataccggtt gaaggcgagc gcagcatccc
    23281 gctggcaaag cgtcgcgtag gatcaccgtt gctgtggagc ccgaatgaag aggaagaccg
    23341 acagttacgc gaagactggg aagaattaat ggatatgatt gttctcggtc agattgagcg
    23401 gatcaccgct cgtcacgggg aatatttaca gatacgaccg aaagcagcga atgcgaaagc
    23461 gctgaccgaa gctattggtg cccggggcga acggattctg acgctgccgc gcggatttta
    23521 tttgaagaag aatttcacca gtgcgctact ggcccgtcat tttctgatcc agtagccatc
    23581 gctttgacct gccgctttcc gggcatataa ttaccgcttc atttttttgg cagggctttt
    23641 tagatgttat ttgcatggat aaccgatcct aacgcctggc ttgcgctcgg tacactgacg
    23701 ctgctggaga tcgttcttgg gatcgacaat attattttcc tttctctggt ggtggcaaag
    23761 cttcccacag cacaacgtgc tcatgcgcgc cgtctggggt tggcgggagc catggtcatg
    23821 cgtctggcgc tgctggcatc catcgcctgg gttacgcgcc tgacgaatcc gctttttaca
    23881 atattcagtc aggaaatttc cgcccgtgat ttgatcctgc ttctgggtgg cttgttcctt
    23941 atctggaaag ccagcaagga aatccacgaa tccatcgaag gtgaagaaga agggctgaaa
    24001 acacgcgttt catcattcct cggcgctatc gtgcagatta tgctgctgga tattatcttc
    24061 agcctcgact cggtgattac cgctgtgggt ctgtcagatc acctgtttat tatgatggct
    24121 gccgtggtga ttgccgtagg cgtgatgatg ttcgccgcgc gctcgattgg tgattttgtc
    24181 gagcgccatc cttcggtaaa aatgctggcg ctctctttcc tgattctggt gggctttacc
    24241 ctgattctgg aaagtttcga catccacgta ccgaaaggtt acatctactt cgcgatgttc
    24301 ttctctattg cggttgaaag cctcaacctg attcgcaaca aaaagaatcc gctctgatac
    24361 tccgtacgct ctcctgcggg agggcgtact cactcctgtt accctcacag tttaagattt
    24421 tcctgctttc aagatatata acgtcggttt ataaacagac tattatcata ggtgggccaa
    24481 atgaggataa ccggatgaaa aaatgggcag taataatttc tgcagtcgga ctggcgtttg
    24541 ctgtttccgg gtgttccagt gattacgtca tggcgaccaa agatggccgt atgattttga
    24601 ccgatggaaa acctgaaatt gatgatgata ccgggctggt gagttatcac gatcagcaag
    24661 gtaacgcgat gcaaattaac cgtgatgatg tttcgcaaat tattgaacgt taacaaataa
    24721 ggtcagcatc cggctggcct taagattttt ctctttccct tttcccttcc ctctgccatt
    24781 tttatattcc ttatgtcgtg attataaaaa ggaaacggct atgcaatatc accgtatacc
    24841 ccacagttcg ctggaagtca gcacgctggg gcttggcacg atgacgtttg gtgaacagaa
    24901 cagcgaagcc gacgcccacg cacaactcga ctatgccgtc gctcagggca ttaaccttat
    24961 cgacgttgcc gaaatgtacc cagtacctcc gcgccccgaa actcaagggc taaccgaaac
    25021 ctacgtcggc aactggctgg cgaaacatgg cagccgcgaa aagttaatta tcgcctccaa
    25081 agtgagcgga ccgtcgcgca ataatgacaa aggcatccgc ccggatcagg cgctggatcg
    25141 gaagaatatc cgcgaagcgc tgcatgacag cctcaagcgt ctgcagactg attacctcga
    25201 tctttatcag gtgcactggc cgcagcgccc aaccaactgc ttcggcaaac tcggttatag
    25261 ctggacagat tctgcgcctg cggtttcgct gctggataca ctggacgcac tggcagagta
    25321 ccaacgcgcg ggaaaaattc gttatatcgg cgtgtcgaac gaaactgcat ttggcgtaat
    25381 gcgctacctg catctggcgg acaaacacga tctgccgcgt attgtcacca ttcagaaccc
    25441 ctacagtctg ttaaaccgca gttttgaagt aggtctggca gaagtcagcc agtatgaagg
    25501 ggtcgaactg ctggcctatt cgtgcctggg tttcggcacg ctgaccggga aatatctcaa
    25561 cggtgcaaaa cccgctggcg cacgtaatac gctctttagt cggttcaccc gctatagcgg
    25621 tgagcaaacg caaaaagccg tcgcggcgta tgttgatata gccagacgtc atggcctgga
    25681 tcctgcacag atggcgctcg cttttgtacg ccgtcaaccg tttgttgcca gcactctgct
    25741 gggcgcaacc acgatggagc agctgaaaac taacgtcgaa agtttgcatc tggagttaag
    25801 cgaagacgta ttagcagaaa ttgaagcggt gcatcaggtt tacacttatc cggcaccata
    25861 agattaacgc cggttaaaac cggcgttaaa tattaataac ggcgctgcca gatccacagc
    25921 gccgttattg ccagcgcaaa cagcgcaccg aagccaatgc caatgggcac gaccgggatg
    25981 cctaccatta ccgccagcga gtaaatgccc agcatcaaca acatggcgct gttttcgcca
    26041 aggttttgta ctgcaatcgc attccccgcc ccgacgcttt ttttaccccg ttcctgtagc
    26101 aacgcattga gcggaacgac aaaaaagccc cccagcacgc caatcagcat caacaaggca
    26161 taggctgaca gcagctcgtg ttgcagggag aaaatcagta ccaccacgcc aatcaaaatc
    26221 ccggctggca tacagcgtga cacggtttcc agcgtaacta acttcgctgc cgcaccagcg
    26281 ccaaccacga taccaatcgc taccatcgcg ttgagatagg tcggcgtagc gttatcggta
    26341 atgcccagcg ccaccggtac ccacagcacc aacaggaaac gcagcgtgac acccgctccc
    26401 cagaataaac tggtgcccac cagcgaaaaa cgcgtttcac cattgcgcca tagcgaggtg
    26461 caggcattca ggaaactgcg ggtcatgctg atgagattcc aggactgccc cggacgcgcc
    26521 gccgccagtt taggaatgta gatattggca acgaccgcac caccgtaggc cagtgcgcat
    26581 gcggccaggg cgaccaggac atgccagtca gccagtacac caccggctac ggaaccgagc
    26641 aaaatcgccg ctatggtaga agcttccatt aaaccattag ctttcactaa cttactaccc
    26701 gtggttaatt cgccgagaat accgtatttc gccggtgaat aggctgcagc accaacaccc
    26761 accagcgtat agccgagaaa cggattgata ccaaagcaga tactggctgc gcccagcagc
    26821 ttcaggccgt tggcaaacat catcacccgg cctttggcga agctatccgc cacctgtccg
    26881 acaaacggcg caaaaagaat gtaagcacct acaaacacca tttgcaggat gggctggctc
    26941 cactccggat aaaactgcgc tttcagtaac gccagagtgg caaacagcag cgcattatcg
    27001 ccaaacgcag agagaaactg cgccacgata accgctttca tccccttcga ccacaacgaa
    27061 gtgttagtgt gcactgactc actcatcgtg ttgttccgct tcgtctaccc agcttttcaa
    27121 cgtgacaaag tcaggtttgc cgctgccaag taatggcatc tgtttcagat agcgaatatc
    27181 gcgcggtaca gcaagctccg gcacgccgtg ctcgcgggca tactgttgca acttatcgcg
    27241 cgtcagttcg ttatctgtgg tgaaaagcac cagtgcctcg cctttgctgg catcgctctt
    27301 aatcgcagtg gcatggactt tatctggcga aacaccaagt gccagttgtt ccaccatttc
    27361 cagcgacacc atttcgcctg caattttggc aaagcgtttt gcgcggccct gaatctgcac
    27421 aaagccctgc tcgtcgaaac gcacaatatc gccagtgtca taccagccgc gctccatttc
    27481 gccgcgaaca ttctcggcgg tgggcacttc cagtacacct ggcttctcca cccgcagata
    27541 gccgttcatt atgttcggcc ctttcagttg caggcgtccg ccctcttcga taccaggaac
    27601 cgacaacagg cgcgcatcca ttcctggtag aatacgccct accgtaccgg gtttcgccgc
    27661 catcggtacg ttgatagaaa cgacaggcgc gcactcggtc acaccgtagc cttcaaggat
    27721 gcgcaggcca aatttatcct gccaaagctg tttggtactt tcttgtagtt tttctgcgcc
    27781 tgccaccaca tagcgtagac ggtagaagtc atacgggttg gcgaaacgcg cgtagtgacc
    27841 gagaaaagtc gaggtaccga acaacacggt gcaactgcgg tcatacacca actccggcac
    27901 aatgcggtaa tgcagcgggc ttggataaag gaacacctct gcacctgtga gcagtggcgt
    27961 aaacaggcct accgtcagcc caaaggagtg aaacagcggt aacgccgaca taaagcgatc
    28021 gttggtggtg aagtcggcaa tcgttttaat ctgctcgaca ttcgccagaa tgcttttatg
    28081 gctatggacg acgcctttcg gatggccttc agaaccggag gtaaaaagga tcagcgcctc
    28141 ttcttccggc tgctgtttta cctgtgccag acgcggcatc agcaaatgag cgaagatcca
    28201 tactttgtcg gcagtggtga catctgcttt taaatcttcc agatagaccc agcgcacctg
    28261 agtaagttgc tccggcagat gccagagttt gcctttatcg agaaactgac gggaagtgaa
    28321 aatggttttg atttcagccg ccgtaatagc actggtcaac ccttttaccc cggcggtgta
    28381 gttcatcatt gcgggaatgc ggcgacgggc gatggcccca aaaatcactg ccgcactgat
    28441 gcctgcattg ggcagcatta agccgatgcg ttcgccttca acactgtatt tttcaaggat
    28501 gcgtccaaca aacagcgttt tcgtaagcaa tttgcgatag gagtccgggg taaagttaac
    28561 gtcttcgaca catttcttcc cggctccgaa gcggtacatt gcgctcagta aagattcgta
    28621 cagcgtttca cgcgggcgca ccgccatacg cgcttccatc attatttgat gcagcatttc
    28681 gccagcgatt ttgcgacggt cacgggcacg cggcgcatcc ggcatctcca cctgcgttgg
    28741 tggcaaaata tgcagagtaa tttgcgggaa caagcgacgt ttaaccagac ctttcaggcg
    28801 gctgaagtgc gtaagttccg ccccttcaat acgcacagga ataaccgttg caccagactt
    28861 cgccgcgaca aatcccgcgc catcgtagat tttcatcagc gagccagtgg tggtgatgcg
    28921 tccttcaggg aaaatcacca ctggtcggcc ctgttccacc agacgtacca gatgtttaat
    28981 cgccataggt tgcgtcgggt cgagaggaac aaagtcgata aatgatttca gccaacgcat
    29041 ataccactgt tggcttattg aggtgtaaac ggcaaatacc ggacgtacgg gtaaaaacaa
    29101 tgccagcagt atgccatcaa taaaagagac gtgattaggc gtgattagaa cgcgctcgcc
    29161 cttcagtgcc tgggtgtcac ccgtaacgcg aacgcgatac aaaacacggc acaaatttcg
    29221 aaaaaagcta aaaagcattc caactccctt tgctctgatt cagtaaaagc gaatggaggg
    29281 agattacacg agataaagaa cgcgagcgac agtaaattag gtgcgaaaaa aaacctgcgc
    29341 atccgcgcag gttggtgcaa gagacagggt acgaagagcg taccgaataa tctcaccaat
    29401 caatacctct gggatcttga ttgtggtctg cacgacgact cttcgccagt gagaaaacgc
    29461 aaaggaatga agggaaatgc aacgaggtgt gtaaattgtc ggttactgtt acagattgat
    29521 gaccg
//
LOCUS       DAZFLD010000034        29426 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.034, whole genome shotgun sequence.
ACCESSION   DAZFLD010000034 DAZFLD010000000
VERSION     DAZFLD010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29426
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.034"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..187
                     /locus_tag="ACHKBL_001297"
     CDS             <1..187
                     /locus_tag="ACHKBL_001297"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5818190.1"
                     /translation="IVTLPAIPLAVTLPPALLESQYSITIMSPYIRKLISAITLSHQR
                     LYVERVLKIKLNQCIGS"
     gene            387..1526
                     /gene="wza"
                     /locus_tag="ACHKBL_001298"
     CDS             387..1526
                     /gene="wza"
                     /locus_tag="ACHKBL_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein Wza"
                     /protein_id="HFO5818191.1"
                     /translation="MMKSKMKLMPLLVSVTLISGCTVLPGSNMSTMGKDVIKQQDADF
                     DLDKMVNVYPLTPRLIDQLRPRPNVARPNMTLESEIANYQYRVGPGDVLNVTVWDHPE
                     LTTPAGQYRSSSDTGNWVQPDGTMFYPYIGKVHVVGKTLAEIRSDITGRLATYIADPQ
                     VDVNIAAFRSQKAYISGQVNKSGQQAITNVPLTILDAINAAGGLTDTADWRNVVLTHN
                     GREERISLQALMQNGDLNQNRLLYPGDILYVPRNDDLKVFVMGEVKKQSTLKMDFSGM
                     TLTEALGNAEGIDMTTSNASGIFVIRPLKGEGGRNGKIANIYQLDMSDATSLVMATEF
                     RLQPYDVVYVTTAPVSRWNRLINQLLPTISGVRYMTDTASDIHNW"
     gene            1532..1975
                     /gene="wzb"
                     /locus_tag="ACHKBL_001299"
     CDS             1532..1975
                     /gene="wzb"
                     /locus_tag="ACHKBL_001299"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase Wzb"
                     /protein_id="HFO5818192.1"
                     /translation="MFNNILVVCVGNICRSPTAERLLQRYHPELKVESAGLGALVGKG
                     ADPTAISVAAEHQLSLEGHCARQISRRLCRNYDLILTMEKRHIERLCEMAPEMRGKVM
                     LFGHWDNECEIPDPYRKSRETFVAVYTLLERSARQWAQALNAEQV"
     gene            1978..4140
                     /gene="wzc"
                     /locus_tag="ACHKBL_001300"
     CDS             1978..4140
                     /gene="wzc"
                     /locus_tag="ACHKBL_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase Wzc"
                     /protein_id="HFO5818193.1"
                     /translation="MTEKVKQHAAPVTGSDEIDIGRLVGTVIEARWWVIGITAVFALC
                     AVVYTFFATPIYSADALVQIEQSSGNSLVQDIGSALANKPPASDAEIQLIRSRLVLGK
                     TVDDLDLDIAVSKNTFPIFGAGWDRLMGRQNETVKVTTFNRPKEMADQVFTLNVLDDK
                     NYTLSSDGGFSARGQAGQMLKKEGVTLMVEAIHASPGSEFTVTKYSTLGMINQLQNSL
                     TVTENGKDAGVLSLTYTGEDREQIRDILNSIARNYQEQNIERKSAEASKSLAFLAQQL
                     PEVRSRLDVAENKLNAFRQDKDSVDLPLEAKAVLDSMVNIDAQLNELTFKEAEISKLY
                     TKVHPAYRTLLEKRQALEDEKAKLNGRVTAMPKTQQEIVRLTRDVESGQQVYMQLLNK
                     EQELKITEASTVGDVRIVDPAITQPGVLKPKKGLIILGAIILGLMLSIVGVLLRSLFN
                     RGIESPQVLEEHGISVYASIPLSEWQKARDSVKTIKGIKRYKQSQLLAVGNPTDLAIE
                     AIRSLRTSLHFAMMQAQNNVLMMTGVSPSIGKTFVCANLAAVISQTNKRVLLIDCDMR
                     KGYTHELLGTNNVNGLSEILIGQGEITTAAKPTSIAKFDLIPRGQVPPNPSELLMSER
                     FAELVNWASKNYDLVLIDTPPILAVTDAAIVGRHVGTTLMVARYAVNTLKEVETSLSR
                     FEQNGIPVKGVILNSIFRRASAYQDYGYYEYEYKSDAK"
     gene            4318..5157
                     /gene="wcaA"
                     /locus_tag="ACHKBL_001301"
     CDS             4318..5157
                     /gene="wcaA"
                     /locus_tag="ACHKBL_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaA"
                     /protein_id="HFO5818194.1"
                     /translation="MKDNPLISIYMPTWNRQQLAIRAIKSVLRQDYSNWEMIIVDDCS
                     TSWEQLQQYVTALNDPRITYIHNDINSGACAVRNQAIMLAQGEYITGIDDDDEWTPNR
                     LSVFLAHKQQLVTHAFLYANDYVCQGEVYSQPASLPLYPKSPYSRRLFYKRNIIGNQV
                     FTWAWRFKECLFDTELKAAQDYDIFLRMVVEYGEPWKVEEATQILHINHGEMQITSSP
                     KKFSGYFHFYRKHKDKFDRASKKYQLFTLYQIRNKRMTWRTLLTLLSVRNGKRLADGI
                     RGR"
     gene            5160..5648
                     /gene="wcaB"
                     /locus_tag="ACHKBL_001302"
     CDS             5160..5648
                     /gene="wcaB"
                     /locus_tag="ACHKBL_001302"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaB"
                     /protein_id="HFO5818195.1"
                     /translation="MLEDLRANSWSLRPCCMVLAYRAAHFCSVWRKKNVLNNLWAAPL
                     LVLYRIITECFFGYEIQAAATIGRRFTIHHGYAVVINKNVVAGDDFTIRHGVTIGNRG
                     ADNMACPHIGNGVELGANVIILGDITLGNNVTVGAGSVVLDSVPDNALVVGEKARVKV
                     IK"
     gene            5645..6862
                     /gene="wcaC"
                     /locus_tag="ACHKBL_001303"
     CDS             5645..6862
                     /gene="wcaC"
                     /locus_tag="ACHKBL_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaC"
                     /protein_id="HFO5818196.1"
                     /translation="MNILQFNVRLAEGGAAGVALDLHQRALQQGLASHFVYGYGKGGK
                     ESVSHQNYPQVIKHTPRMTAMANIALFRLLNRDLFGNFNELYRTITRTPGPVVLHFHV
                     LHSYWLNLKSVVRFCEKVKNHKPDVTLVWTLHDHWSVTGRCAFTDGCEGWKTGCQKCP
                     TLNNYPPVKIDRAHQRVAGKRQLFSEMLALGCQFISPSQHVADAFNSLYGPGRCRIIN
                     NGIDMATEAILADLPPVRETQGKPKIAVVAHDLRYDGKTNQQLVREMMALGDKIELHT
                     FGKFSPFTAGNVVNHGFETDKRKLMSALNQMDALVFSSRVDNYPLILCEALSIGVPVI
                     ATHSDAAREVLQKSGGKTVSEEDVLQLVQSSKPEIAQAIFGTTLAEFSQRSRAAYSGQ
                     QMLEEYVNFYQNL"
     gene            6837..8054
                     /gene="wcaD"
                     /locus_tag="ACHKBL_001304"
     CDS             6837..8054
                     /gene="wcaD"
                     /locus_tag="ACHKBL_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid polymerase WcaD"
                     /protein_id="HFO5818197.1"
                     /translation="MSTSIRICSYLLLPLIYLLVNVKIAQLGESFPITIVTFLPVLLL
                     LFLERISVKKLMIALGIGAGLTAFNYLFGQSLDAGKYVTSTMLFVYIVIIIGMVWSIR
                     FKTISPHNHRKILRFFYLVVGLVVVLAAVEMAQIILTGGSSIMESISKYLIYSNSYVL
                     NFIKFGGKRTTALYFEPAFFALALISIWLSIKQFGIKTPKTDAMILAGIILSGSFSGV
                     MTFILFYLLEWAFQYLNKEAIKKKLPLALISLAVFLVGVVIAFPYISTRLGDLGTEGS
                     SSYYRIVGPLVMVGYSLTHIDGVVRFGSLYEYVASFGIFNGADVGKTIDNGLYLLIIY
                     FSWFAVFLSLWYMGKVIKMMINAFGDNRNFRVQLYLFTPVSLFFTGSIFSPEYAFLIV
                     CPFILRKALNITR"
     gene            8065..8811
                     /gene="wcaE"
                     /locus_tag="ACHKBL_001305"
     CDS             8065..8811
                     /gene="wcaE"
                     /locus_tag="ACHKBL_001305"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaE"
                     /protein_id="HFO5818198.1"
                     /translation="MLLSIITVAFRNLAGIVKTHASLAHLAQADDISFEWIVVDGGSN
                     DGTREYLENLNGIYNLRFVSEPDNGIYDAMNKGIAMAHGKFALFLNSGDIFHQDAANF
                     VRKLKVQKDNMMITGDALLDFGDGHKIKRSAKPGWYIYHSLPASHQAIFFPVSGLKKW
                     RYDLEYKVSSDYALAAKMYKAGYAFKKLNGLVSEFSMGGVSTTNNMELCADAKKVQRQ
                     ILHVPGFWAELSWHLRQRTTSKTKALYNKS"
     gene            8827..9375
                     /gene="wcaF"
                     /locus_tag="ACHKBL_001306"
     CDS             8827..9375
                     /gene="wcaF"
                     /locus_tag="ACHKBL_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaF"
                     /protein_id="HFO5818199.1"
                     /translation="MQDLSGFSVPKGFRGGNAIKVQLWWAVQATIFAWSPQVLYRWRA
                     FLLRLFGAKIGKNVVIRPSVKITYPWKLTLGDYAWVGDDVNLYTLGEITIGAHSVISQ
                     KSYLCTGSHDHASQHFTINATPIVIGEKCWLATDVFVAPGVTIGNGTVVGARSSVFKS
                     LPANVVCRGNPAVVIRERVETE"
     gene            9402..10523
                     /gene="gmd"
                     /locus_tag="ACHKBL_001307"
     CDS             9402..10523
                     /gene="gmd"
                     /locus_tag="ACHKBL_001307"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0019673 - GDP-mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HFO5818200.1"
                     /translation="MSKVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERV
                     DHIYQDPHTCNPKFHLHYGDLSDTSNLTRILREVQPDEVYNLGAMSHVAVSFESPEYT
                     ADVDAMGTLRLLEAIRFLGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVA
                     KLYAYWITVNYRESYDMYACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYL
                     GNMDSLRDWGHAKDYVKMQWMMLQQEQPEDFVIATGVQYSVRQFVEMAAAQLGIKLRF
                     EGTGVEEKGIVVSVTGHDAPGVKPGDVIIAVDPRYFRPAEVETLLGDPTKAHEKLGWK
                     PEITLREMVSEMVANDLEAAKKHSLLKSHGYDVAIALES"
     gene            10526..11491
                     /gene="fcl"
                     /locus_tag="ACHKBL_001308"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
     CDS             10526..11491
                     /gene="fcl"
                     /locus_tag="ACHKBL_001308"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /EC_number="1.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HFO5818201.1"
                     /translation="MSKQRIFIAGHRGMVGSAIRRQLEQRGDVELVLRTRDELNLLDS
                     RAVHDFFASESIDQVYLAAAKVGGIVANNTYPADFIYQNMMIESNIIHAAHQNDVNKL
                     LFLGSSCIYPKLAKQPMAESELLQGTLEPTNEPYAIAKIAGIKLCESYNRQYGRDYRS
                     VMPTNLYGPHDNFHPSNSHVIPALLRRFHEATAQNAPDVVVWGSGTPMREFLHVDDMA
                     AASIHVMELAHEVWLENTQPMLSHINVGTGVDCTIRELAQTIAKVVGYKGRVVFDASK
                     PDGTPRKLLDVTRLHQLGWYHEISLEAGLASTYQWFLENQDRFRG"
     gene            11494..11973
                     /gene="gmm"
                     /locus_tag="ACHKBL_001309"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
     CDS             11494..11973
                     /gene="gmm"
                     /locus_tag="ACHKBL_001309"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HFO5818202.1"
                     /translation="MFLRQEDFATVVRSTPLVSLDFIVENSRGEFLLGKRTNRPAQGY
                     WFVPGGRVQKDETLEAAFERLTMAELGLRLPITAGQFYGVWQHFYDDNFSGTDFTTHY
                     VVLGFRFRVVEEELLLPDEQHDDYRWLTPDALLASDNVHANSRAYFLAEKRAGVPGL"
     gene            11970..13193
                     /gene="wcaI"
                     /locus_tag="ACHKBL_001310"
     CDS             11970..13193
                     /gene="wcaI"
                     /locus_tag="ACHKBL_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis fucosyltransferase
                     WcaI"
                     /protein_id="HFO5818203.1"
                     /translation="MKILVYGINYSPELTGIGKYTGEMVEWLAAQGHEVRVITAPPYY
                     PQWQVGENYSAWRYKREEGAATVWRCPLYVPKQPSTLKRLLHLGSFAVSSFFPLMAQR
                     RWQPDRIIGVVPTLFCTPGMRLLAKLSGARTVLHIQDYEVDAMLGLGLAGKGKGGKVA
                     QLATAFERSGLHNVDNVSTISRSMMNKAIEKGVAAENVIFFPNWSEIARFQHVANADV
                     DALRNQLGLPDNKKIILYSGNIGEKQGLENVIEAADRLRDEPLIFAIVGQGGGKARLE
                     KMAQQRGLRNMQFFPLQSYDALPALLKMGDCHLVVQKRGAADAVLPSKLTNILAVGGN
                     AVITAEAHTELGQLCETFPGIAVCVEPESVEALVAGIRQALLLPKHNTVAREYAERTL
                     DKENVLRQFINDIRG"
     gene            13196..14632
                     /gene="cpsB"
                     /locus_tag="ACHKBL_001311"
     CDS             13196..14632
                     /gene="cpsB"
                     /locus_tag="ACHKBL_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
                     /protein_id="HFO5818204.1"
                     /translation="MAQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGDLTMLQTT
                     ICRLNGVECESPVVICNEQHRFIVAEQLRQLNKLTENIILEPAGRNTAPAIALAALAA
                     KRHSPESDPLMLVLAADHVIADEDAFRTAVRNAMPYAEAGKLVTFGIVPDLPETGYGY
                     IRRGEVSAGEQDTVAFEVAQFVEKPNLETAQAYVASGEYYWNSGMFLFRAGRYLEELK
                     KYRPDILDACEKAMSAIDPDLDFIRVDEEAFLACPEESVDYAVMERTADAVVVPMDAG
                     WSDVGSWSSLWEISAHTAEGNVCHGDVINHKTENSYVYAESGLVTTVGVKDLVVVQTK
                     DAVLIADRNAVQDVKKVVEQIKADGRHEHRVHREVYRPWGKYDSIDAGDRYQVKRITV
                     KPGEGLSVQMHHHRAEHWVVVAGTAKVTIDGDIKLLGENESIYIPLGATHCLENPGKI
                     PLDLIEVRSGSYLEEDDVVRFADRYGRV"
     gene            14825..16195
                     /gene="cpsG"
                     /locus_tag="ACHKBL_001312"
                     /gene_synonym="manB"
     CDS             14825..16195
                     /gene="cpsG"
                     /locus_tag="ACHKBL_001312"
                     /gene_synonym="manB"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis phosphomannomutase
                     CpsG"
                     /protein_id="HFO5818205.1"
                     /translation="MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDARLTSETLKLALAKGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARPISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     NNPGAKIIHDPRLSWNTVDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGELVRDRMAAFPASGEINSKLADPVEAINR
                     VEQHFSREALAVDRTDGISMTFADWRFNLRTSNTEPVVRLNVESRGDVPLMEARTRTL
                     LTLLNE"
     gene            16250..17644
                     /gene="wcaJ"
                     /locus_tag="ACHKBL_001313"
     CDS             16250..17644
                     /gene="wcaJ"
                     /locus_tag="ACHKBL_001313"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HFO5818206.1"
                     /translation="MTNLKKRERAKTNASLISMVQRFSDITIMFAGLWLVCEVSGLSF
                     LYMHLLVALITLVVFQMLGGITDFYRSWRGVRAATEFALLLQNWTLSVIFSAGLVAFN
                     NDFDTQLKIWLAWYGLTSIGLVVCRSCIRIGAGWLRNHGYNKRMVAVAGDLAAGQMLM
                     ESFRNQPWLGFEVVGVYHDPKPGGVSNDWAGNLQQLVEDAKAGKIHNVYIAMQMCDGA
                     RVKKLVHQLADTTCSVLLIPDVFTFNILHSRIEEMNGVPVVPLYDTPLSGVNRLLKRA
                     EDIVLATLILLLISPVLCCIALAVKLSSPGPVIFRQTRYGMDGKPIKVWKFRSMKVME
                     NDKVVTQATQNDPRVTKVGNFLRRTSLDELPQFINVLTGGMSIVGPRPHAVAHNEQYR
                     QLIEGYMLRHKVKPGITGWAQINGWRGETDTLEKMEKRVEFDLEYIREWSVWFDIKIV
                     FLTVFKGFVNKAAY"
     gene            17646..19124
                     /gene="wzxC"
                     /locus_tag="ACHKBL_001314"
     CDS             17646..19124
                     /gene="wzxC"
                     /locus_tag="ACHKBL_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid undecaprenyl disphosphate flippase
                     WzxC"
                     /protein_id="HFO5818207.1"
                     /translation="MNLREKTISGAKWSAIATVIIIGLGLVQMTVLARIIDNHQFGLL
                     TVSLVIIALADTLSDFGIANSIIQRKEISHLELTTLYWLNVGLGIVVCVAVFLLSDTI
                     GDVLNNPDLAPLIKTLSLAFVVIPHGQQFRALMQKELEFNKIGMIETSAVLAGFTFTV
                     VSAHFWPLAMTAILGYLVNSAVRTLLFGYFGRKIYRPGLHFSLASVAPNLRFGAWLTA
                     DSIINYLNTNLSTLVLARILGAGVAGGYNLAYNVAVVPPMKLNPIITRVLFPAFAKIQ
                     DDTEKLRVNFYKLLSVVGIINFPALLGLIVVSNNFVPLVFGEKWNSIIPVLQLLCVVG
                     LLRSVGNPIGSLLMAKARVDISFKFNVFKTFLFIPAIVIGGQMAGAIGVTLGFLLVQI
                     INTILSYFVMIKPVLGSSYRQYILSLWLPFYLSLPTLVVSYALGIVLKGQLALGMLLA
                     VQIATGVLAFVVMIVLSRHPLVVEVKRQFCRSEKMKMLLRAG"
     gene            19785..21065
                     /gene="wcaK"
                     /locus_tag="ACHKBL_001315"
     CDS             19785..21065
                     /gene="wcaK"
                     /locus_tag="ACHKBL_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis pyruvyl transferase
                     WcaK"
                     /protein_id="HFO5818208.1"
                     /translation="MKLLILGNHTCGNRGDSAILRGLLDAINILNPHAEVDVMSRYPV
                     SSSWLLNRPVMGDPLFLQMKQHNSAAGVVGRVKKVLRRRYQHQVLLSRVTDTGKLRNI
                     AIAQGFTDFVRLLSGYDAIIQVGGSFFVDLYGVPQFEHALCTFMAKKPLFMIGHSVGP
                     FQDEQFNQLANYVFGHCDALILRESVSLDLMKRSNITTAKVEHGVDTAWLVDHHIEDF
                     TASYAVQHWLDVAAQQKTVAITLRELAPFDKRLGTTQQAYEKAFAGVVNRILDEGYQV
                     IALSTCTGIDSYNKDDRMVALNLRQHISDPARYHVVMDELNDLEMGKILGACELTVGT
                     RLHSAIISMNFATPAIAINYEHKSAGIMQQLGLPEMAIDIRHLLDGSLQAMVADTLGQ
                     LPALNTRLNEAVSRERQTGMQMVQSVLERIGEVK"
     gene            21062..22282
                     /gene="wcaL"
                     /locus_tag="ACHKBL_001316"
     CDS             21062..22282
                     /gene="wcaL"
                     /locus_tag="ACHKBL_001316"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaL"
                     /protein_id="HFO5818209.1"
                     /translation="MKVGFFLLKFPLSSETFVLNQITAFIDMGFEVEIIALQKGDTQN
                     THAAWTKYNLAARTRWLQDEPTGKVAKLRHRASQTLCGIHRKNTWQALNLKRYGAESR
                     NLILSAICGQVATPFHADVFIAHFGPAGVTAAKLRELGVIRGKIATIFHGIDISSREV
                     LNHYTPEYQQLFRRGDLMLPISDLWAGRLQKMGCPREKIAVSRMGVDMTRFSPRPVKA
                     PATPLEIISVARLTEKKGLHVAIEACRQLKEQGVAFRYRILGIGPWERRLRTLIEQYQ
                     LEDVIEMPGFKPSHEVKAMLDDADVFLLPSVTGADGDMEGIPVALMEAMAVGIPVVST
                     LHSGIPELVEADKSGWLVPENDARALAQRLAAFSQLDTDELAPVIKRAREKVEHDFNQ
                     QVINRELASLLQAL"
     gene            22293..23687
                     /gene="wcaM"
                     /locus_tag="ACHKBL_001317"
     CDS             22293..23687
                     /gene="wcaM"
                     /locus_tag="ACHKBL_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis protein WcaM"
                     /protein_id="HFO5818210.1"
                     /translation="MPFKKLSRRTFLTASSALAFLHTPFARALPARQSVNINDYNPHD
                     WIASFKQAFSEGQTVVVPAGLVCDNINTGIFIPPGKTLHILGSLRGNGRGRFVLQDGS
                     QVTGEEGGSMHNITLDVRGSDCIIKGLAMSGFGPVTQIYIGGKNKRVMRNLTIDNLTV
                     SHANYAILRQGFHNQIIGANITNCKFSDLQGDAIEWNVAINDRDILISDHVIERINCT
                     NGKINWGIGIGLAGSTYDNNYPEDQAVKNFVVANITGSDCRQLIHVENGKHFVIRNIK
                     ARNITPDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGYGVIKGKYLSIPQNF
                     RVNNIQLDNTHLAYKLRGIQISAGNAVSFVALTNIEMKRASLELHNKPQHLFMRNIKV
                     MQESSVGPALSMNFDMRKDVRGVFMAKKETLLSLANVHAMNERGQSSVDIDRVNHHIV
                     NVEKINFRLPERRE"
     gene            23845..24840
                     /locus_tag="ACHKBL_001318"
     CDS             23845..24840
                     /locus_tag="ACHKBL_001318"
                     /EC_number="5.1.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,
                     octacis-undecaprenol 4-epimerase"
                     /protein_id="HFO5818211.1"
                     /translation="MNDNVLLIGASGFVGTRLLETAIADFNIKNLDKQQSHFYPEITQ
                     IGDVRDQQALDQALAGFDTVVLLAAEHRDDVSPTSLYYDVNVQGTRNVLTAMEKNGVK
                     NIIFTSSVAVYGLNKHNPDENHPHDPFNHYGKSKWQAEEVLREWYNKAPTERSLTIIR
                     PTVIFGERNRGNVYNLLKQIAGGKFMMVGAGTNYKSMAYVGNIVEFIKYKLKNVAAGY
                     EVYNYVDKPDLNMNQLVAEVEQSLNKKIPSMHLPYPLGMLGGYCFDILSKITGKKYAV
                     SSVRVKKFCATTQFDATKVHSSGFVAPYTLSQGLDRTLQYEFVHAKKDDITFVSE"
     gene            25083..25976
                     /gene="galF"
                     /locus_tag="ACHKBL_001319"
     CDS             25083..25976
                     /gene="galF"
                     /locus_tag="ACHKBL_001319"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalF"
                     /protein_id="HFO5818212.1"
                     /translation="MTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEI
                     VAAGIKEILLVTHASKNAVENHFDTSYELESLLEQRVKRQLLAEVQSICPPGVTIMNV
                     RQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNETGRS
                     QVLAKRMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGRYVLS
                     ADIWPELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQAFVKY
                     GLRNLKEGAKFRKGIEKLLSE"
     gene            26348..27433
                     /gene="rfbB"
                     /locus_tag="ACHKBL_001320"
     CDS             26348..27433
                     /gene="rfbB"
                     /locus_tag="ACHKBL_001320"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFO5818213.1"
                     /translation="MKILVTGGAGFIGSAVVRHIINNTQDSVVNVDKLTYAGNLESLA
                     DVSDSERYIFEHADICDAAAMARIFAQHQPDAVMHLAAESHVDRSITGPAAFIETNIV
                     GTYVLLEAARNYWSALDGDKKNSFRFHHISTDEVYGDLPHPDEVNNKEQLPLFTETTA
                     YAPSSPYSASKASSDHLVRAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEG
                     KALPIYGKGDQIRDWLYVEDHARALYTVVTEGKAGETYNIGGHNEKKNIDVVLTICDL
                     LDEIVPKEKSYREQITYVADRPGHDRRYAIDAEKIGRELGWKPQETFESGIRKTVEWY
                     LSNTKWVDNVKSGAYQSWIEQNYEGRQ"
     gene            27433..28332
                     /gene="rfbD"
                     /locus_tag="ACHKBL_001321"
     CDS             27433..28332
                     /gene="rfbD"
                     /locus_tag="ACHKBL_001321"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HFO5818214.1"
                     /translation="MNILLFGKTGQVGWELQRALAPLGNLIALDVHSTDYCGDFSNPE
                     GVAETVKKIRPDVIVNAAAHTAVDKAESEPEFAQLLNATSVESIAKAANEVGAWVIHY
                     STDYVFPGTGEIPWLETDATAPLNVYGETKLAGEKALQEQCAKHLIFRTSWVYAGKGN
                     NFAKTMLRLAKEREELAVINDQFGAPTGAELLADCTAHAIRVALNKPEVAGLYHLVAS
                     GTTTWHDYAALVFEEARKAGIPLALNKLNAVATTAYPTPARRPHNSRLNTEKFQQNFA
                     LVLPDWQVGVKRMLNELFTTTVI"
     gene            28390..29268
                     /gene="rfbA"
                     /locus_tag="ACHKBL_001322"
     CDS             28390..29268
                     /gene="rfbA"
                     /locus_tag="ACHKBL_001322"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFO5818215.1"
                     /translation="MKTRKGIILAGGSGTRLYPVTMAVSKQLLPIYDKPMIYYPLSTL
                     MLAGIRDILIISTPQDTPRFHQLLGDGGQWGLNLQYKVQPTPDGLAQAFIIGEEFIGG
                     DDCALVLGDNIFYGHDLPKLMDVAVNKESGATVFAYHVNDPERYGVVEFDKKGTAISL
                     EEKPLQPKSNYAVTGLYFYDNDVVEMAKNLKPSARGELEITDINRIYMEQGGLSVAMM
                     GRGYAWLDTGTHQSLIEASNFIATIEERQGLKVSCPEEIAYRKGFIDAEQVKVLAEPL
                     KKNAYGQYLLRMIKGY"
     gene            29273..>29426
                     /locus_tag="ACHKBL_001323"
     CDS             29273..>29426
                     /locus_tag="ACHKBL_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase family
                     protein"
                     /protein_id="HFO5818216.1"
                     /translation="MNVIKTEIPEVLIFEPKKFEDSRGYFFESFNQRLFEDAVGKEIL
                     FVQDNQS"
BASE COUNT         7356 a         7127 c         7888 g         7055 t
ORIGIN      
        1 aattgttacc ttgcctgcta ttccgttagc tgtaacactt cctcctgcat tattggagag
       61 ccaatattca attaccataa tgtcacctta tattcggaag ttaatttccg caataaccct
      121 ttctcatcaa cgactgtatg ttgagagggt tttaaaaatt aagctaaatc agtgtattgg
      181 tagctaaaaa gccaggggcg gtagcgtgtc tggatgcctg aaagaccagt ctgaattatt
      241 ctgtcaatag cctgcggatg aaatcaattt ttttaggact gatgccagtt aaatttttat
      301 tcaattaact gcaaggtaaa tgtgcatggc gacattgcta ttaatagtgc acaggataat
      361 tactctgcca aagtgataaa taaacaatga tgaaatccaa aatgaaattg atgccattat
      421 tggtgtcagt aaccttgata agcggttgca cagtacttcc gggcagcaat atgtcgacga
      481 tgggtaaaga cgtcatcaaa cagcaggacg ctgatttcga tctcgacaaa atggtgaatg
      541 tttatccgtt gaccccgcgc ctgattgatc aattacgccc acgcccgaat gtagcgcgcc
      601 ccaatatgac gctggaaagt gagatcgcga attatcagta tcgcgtcggg ccgggggacg
      661 ttcttaatgt caccgtctgg gatcacccgg aactcaccac gccagccggt cagtaccgca
      721 gctccagcga caccggcaac tgggtacagc ctgacggcac catgttctac ccgtatatcg
      781 gcaaggttca cgtcgtgggg aaaacgctcg ctgaaatccg cagtgatatt accggtcgct
      841 tagcgacgta catcgctgac ccgcaggtgg acgttaatat cgccgccttc cgctcgcaaa
      901 aagcctatat ctcaggtcag gtgaataaat ccggtcaaca ggcgattacc aacgtaccac
      961 tgaccattct cgatgccatc aacgccgcag gtggcctgac cgacaccgct gactggcgca
     1021 acgtggtgct aacacacaat ggtcgtgaag aacgcatttc tttgcaggcg ctgatgcaaa
     1081 acggcgacct caatcagaac cgactgcttt accccggcga tattctctac gtgccgcgta
     1141 atgatgatct gaaagtgttt gtgatgggtg aagttaaaaa acagagcacc ctgaaaatgg
     1201 actttagcgg catgacccta actgaagccc tgggtaatgc tgaaggcatc gacatgacca
     1261 cctccaacgc cagcggcatc tttgtcattc gtccgttgaa aggcgagggc gggcgtaacg
     1321 gcaagattgc caatatctac cagctggata tgtccgatgc cacatcgctg gtgatggcga
     1381 cagaattccg cctgcaacct tatgacgtgg tgtatgtcac caccgccccg gtttcccgct
     1441 ggaaccgtct gatcaatcag ttgctgccaa ctattagcgg tgtccgttac atgacggata
     1501 cagccagcga cattcataac tggtaatcgt catgtttaac aacatcttag ttgtctgtgt
     1561 cggcaatatt tgccgttccc cgacggcgga acgcttactg caacgttatc acccggagct
     1621 gaaagtggag tccgctggac tcggcgcgct ggtcggtaag ggcgctgacc ctactgctat
     1681 cagcgtcgcc gcagaacatc aactgtctct ggaaggtcac tgtgcacggc aaatcagccg
     1741 tcgcctgtgt cgcaactacg acctgatttt gaccatggaa aagcgccata tcgaacgctt
     1801 atgcgagatg gcaccggaga tgcgcggcaa agtgatgctg tttggtcact gggataacga
     1861 atgtgaaatc cccgatccgt atcgcaaaag ccgggaaacg tttgtagcgg tgtacacatt
     1921 acttgaacgg tctgcccgcc agtgggcgca ggcattgaac gcagagcagg tataagaatg
     1981 acagaaaaag taaaacaaca tgccgctccg gtaacgggca gtgatgaaat cgatattggt
     2041 cgcctggtcg gcaccgtcat tgaagcgcgc tggtgggtga ttggcatcac cgccgtattt
     2101 gccctctgtg ccgtggttta caccttcttc gctacgccga tatacagcgc cgacgcactg
     2161 gtacaaatcg aacaaagcag cggcaattcg ttagtgcagg atatcggatc ggcattagcc
     2221 aacaaaccgc ctgcatcgga cgccgagatc cagttgattc gttcgcgcct ggtgcttggt
     2281 aaaacggtgg acgatctcga cctcgatatt gcggtgagca aaaacacgtt cccgattttc
     2341 ggtgcgggct gggatcgcct gatgggacgc cagaacgaga cggtaaaagt gaccaccttt
     2401 aaccgtccta aggagatggc ggatcaggtg tttacgctta atgtgcttga tgacaaaaac
     2461 tacactttga gcagcgatgg cggctttagc gcccgtgggc aagcgggcca gatgctgaaa
     2521 aaagaaggcg tcacgctgat ggttgaagct attcacgcca gcccgggcag tgagtttacc
     2581 gtcaccaaat actccacgct ggggatgatc aatcaactgc aaaacagcct gacggtaacg
     2641 gagaacggca aagatgctgg cgttttgagc ctgacttata ctggagaaga tcgcgaacag
     2701 atccgcgaca ttcttaacag tatcgcccgt aactatcagg aacaaaatat tgagcgcaaa
     2761 tcggcggaag cgtcgaaaag cctcgctttc ctcgcgcaac agttaccgga agtacgtagc
     2821 cgcctcgatg ttgccgaaaa caaactgaat gccttccgtc aggataaaga ttcagttgat
     2881 ctgccgctgg aagcgaaagc ggtgctcgat tcgatggtga acatcgacgc gcagttgaac
     2941 gaactgacct ttaaagaagc ggaaatctcc aagctgtaca ccaaagttca cccggcgtat
     3001 cgcacgctgc tggagaaacg gcaggcgctg gaagacgaaa aagccaaact taatggtcgc
     3061 gtaacggcga tgccgaaaac ccagcaggaa attgtccgtc tgacccgcga tgtcgagtct
     3121 gggcagcagg tctatatgca actgctgaat aaagagcagg agctgaaaat caccgaagcc
     3181 agcaccgtcg gcgatgtgcg cattgttgac ccggcaatca cccagcctgg tgtgctaaaa
     3241 ccgaagaaag ggctgattat ccttggggcg attatccttg gcctgatgct ctctatcgtg
     3301 ggtgtgctgc tgcgctcgtt gtttaatcgc ggtatcgaaa gtccgcaggt gctggaagaa
     3361 cacggtatca gcgtctatgc cagcatcccg ctgtcggagt ggcagaaagc gcgcgatagc
     3421 gtcaaaacca tcaaagggat taaacgctat aaacagagcc agctactggc ggtggggaat
     3481 ccaaccgatc tggcgattga agccatccgt agtctgcgca ccagtctgca cttcgcgatg
     3541 atgcaggcac agaacaatgt gttgatgatg accggggtta gcccgtcaat cggtaaaacc
     3601 tttgtctgcg ccaacctggc ggcggtgatc agtcagacca ataaacgggt gttgttgatc
     3661 gactgcgata tgcgcaaagg ctacacccac gagctgttgg gcactaataa cgtcaatggc
     3721 ctgtcggaaa ttctgattgg tcagggcgag attactaccg ctgctaaacc gacctctatt
     3781 gccaaatttg acctgatccc gcgcggtcag gtaccgccaa atccttcgga actgttgatg
     3841 agcgaacgtt ttgccgaact ggtgaactgg gcgagtaaaa actacgacct ggtgttgatt
     3901 gatacgccgc cgattctggc agtgaccgat gcggcaattg ttggtcgtca tgtcggaacc
     3961 acgttgatgg tggcgcgtta tgcggtcaac acattgaaag aagtggaaac cagtctgagc
     4021 cgctttgagc aaaacggtat tccggtgaaa ggggtgattc tgaactccat cttccgtcgc
     4081 gccagcgcgt atcaggatta tggctattac gaatacgaat ataagtcgga cgcgaaataa
     4141 cgaaatccgc tgtcggatgc gatgctgacg catcttatcc gacctacgtt cacgccgcat
     4201 caggcaagca aacccgctcc gtaggtcgga taaggcgttc acgccgcatc cggcaacaga
     4261 acctgtaaac cagcttataa accggcagaa catctcgaca ctccgggaga acaacctatg
     4321 aaagacaatc cgctgatttc aatctatatg ccgacctgga accgtcaaca actggcgatt
     4381 cgggcgataa aatcggtcct gcgccaggac tacagtaact gggagatgat catcgtggat
     4441 gattgctcca cctcctggga acaactacaa cagtacgtca ccgccctcaa cgatccgcgt
     4501 atcacttaca ttcacaacga cattaacagc ggggcgtgcg cggtacgtaa ccaggcgatt
     4561 atgctggcgc aaggggaata tatcaccggg atcgatgatg atgatgaatg gacacccaac
     4621 cgtctgagcg tcttcctcgc ccataaacag caactggtca cacacgcctt tttgtacgct
     4681 aacgactacg tttgccaggg cgaagtctat tcccaaccgg caagtttgcc actgtatccc
     4741 aaatcgccgt attcacgccg cctgttctac aaacgcaata tcattggcaa tcaggtcttt
     4801 acctgggcgt ggcgtttcaa agagtgtttg ttcgataccg aactgaaagc ggcacaggat
     4861 tacgacattt tcctgcggat ggtggtggag tatggcgaac cgtggaaagt ggaagaggcg
     4921 acgcagatcc tgcatatcaa tcacggcgaa atgcagatca cctcgtcacc gaaaaaattc
     4981 tccggatact tccactttta ccgtaagcac aaagacaaat ttgaccgcgc cagcaaaaaa
     5041 tatcagctgt ttaccctcta ccagatccgc aataaacgca tgacctggcg caccttgttg
     5101 acgctgctgt cggtgcgtaa cggcaaacgt ctggctgatg gtattcgggg gcgctaaaga
     5161 tgctggaaga tctgcgcgcc aacagctgga gtttacgccc gtgctgcatg gttcttgcct
     5221 atcgcgccgc tcatttttgt tcggtgtggc gcaaaaagaa cgtcctcaac aatctttggg
     5281 cggccccgct gctggtgctg tatcgcatta tcaccgaatg ctttttcggt tatgaaatcc
     5341 aggccgccgc gaccattggc cgccgtttca ctatccatca cggttacgcc gtagtgatca
     5401 ataaaaacgt ggtagcgggg gatgatttca ccattcgcca cggcgtcact atcggcaatc
     5461 gtggtgccga taacatggca tgtccacaca ttggcaacgg cgtcgaactc ggtgccaacg
     5521 tcattattct tggtgatatc acgcttggta acaacgtcac cgtgggcgcg ggcagcgtgg
     5581 tgctcgactc tgtcccggac aacgcgctgg tggtgggaga aaaagcgcga gtgaaggtaa
     5641 ttaaatgaat attctgcaat tcaatgtgcg actggcggaa ggcggggcgg caggtgtggc
     5701 gttagatctc caccagcgcg cgctgcaaca ggggctggcg tcacattttg tctacggcta
     5761 cggcaaaggc ggcaaagaga gcgtcagcca tcaaaactat ccgcaggtca tcaaacatac
     5821 gccgcggatg accgcgatgg cgaacattgc gttgtttcgt ctgcttaatc gcgatctgtt
     5881 cggcaatttc aatgagttat atcgcaccat tactcgcaca ccgggtccgg tggtcctgca
     5941 ttttcatgtg ctgcacagct actggttgaa tcttaagagc gtggtgcgct tttgcgaaaa
     6001 agtgaaaaac cataaaccgg atgtcactct ggtctggacg ttacacgatc actggagcgt
     6061 taccggacgc tgcgccttta ccgacggttg cgaaggctgg aaaacgggct gccagaaatg
     6121 cccgacctta aataattatc cgccggtgaa gattgatcgc gcacaccaac gggtggcggg
     6181 caaacggcag ttattcagcg agatgctggc gctgggctgt cagtttattt cccccagcca
     6241 gcatgtggca gacgctttca atagtctgta cggtccaggg cgttgccgga ttatcaataa
     6301 cggtattgat atggcaaccg aagcgattct ggcggacttg cctccggtgc gcgaaaccca
     6361 gggcaagccg aaaatcgcgg tggtggcgca tgacctgcgt tacgacggca aaactaacca
     6421 gcaactggta cgcgagatga tggcgctggg cgacaaaatc gaactgcata ctttcggtaa
     6481 gttctcgccg ttcaccgctg gtaacgttgt taatcacggc tttgaaactg acaaacgtaa
     6541 actgatgagc gcgctcaacc agatggatgc gctggtgttc agttctcgcg tcgataacta
     6601 cccgctgatt ttgtgtgagg cgttatcgat tggcgtgccg gtgattgcca cccatagcga
     6661 cgcggcgcgg gaagtgctgc aaaaatccgg cggtaaaacc gtcagcgaag aagatgtgct
     6721 gcaactggtg cagtcaagca aaccggaaat cgcacaggcg atatttggta ccacgctggc
     6781 tgagttcagc cagcgcagcc gcgccgccta cagtggacaa cagatgctgg aggagtatgt
     6841 caacttctat cagaatctgt agctacctgc tgctgccgct gatctacctg ctggttaacg
     6901 tcaaaatcgc ccagcttggc gaaagttttc ccatcaccat cgtcactttt ttacctgtct
     6961 tgttactgct gtttttagaa cgcatcagcg ttaaaaaatt gatgattgcc ttagggattg
     7021 gtgcgggact cacggcgttt aactacctgt ttggtcagtc gttggatgcc ggtaaatacg
     7081 tcacttcaac tatgctgttt gtctatattg tgatcattat tggcatggtg tggagtattc
     7141 gctttaaaac gatttcgccc cacaaccacc gcaagatatt acgtttcttt tatctggtgg
     7201 tcgggctggt ggtggtgctg gcggcggtgg agatggcgca aattatcctc accggcggta
     7261 gcagtattat ggagtcgatt tcgaaatatc tgatttacag caacagctat gtgctgaatt
     7321 tcattaaatt cggcggcaag cgcacgacag cactttattt cgaaccggca tttttcgctc
     7381 tggcattaat ctcaatttgg ctcagcatca aacagtttgg tatcaaaacg cctaaaaccg
     7441 atgctatgat tcttgcaggg ataatattat ccggatcgtt ttcaggggtt atgaccttta
     7501 tcctgtttta tttgctggag tgggcatttc aatatctgaa taaagaggcg attaagaaaa
     7561 agttaccgtt agcattgatt tctctggctg tattcctggt tggtgtggta atcgcgtttc
     7621 cttatatttc cacccgtctg ggcgatttag gtacggaagg atcgtcatcg tattatcgta
     7681 ttgtcggtcc gctggtgatg gtcggttatt ctttgaccca tattgacggt gtagtcagat
     7741 ttggctcact ttatgaatat gtcgcatcat tcggaatatt taacggtgcg gatgtcggaa
     7801 aaaccataga caatggtttg tatctgctga ttatttattt ttcctggttc gcagtgtttt
     7861 tatcgctgtg gtacatgggg aaagtgataa aaatgatgat caacgctttt ggtgataacc
     7921 gcaattttcg cgtgcaatta tatcttttta ctccggtatc gctgtttttt accggttcga
     7981 tatttagccc ggaatatgca tttttaatcg tctgtccgtt tattttgcga aaagcgttaa
     8041 atattacgag gtaagaataa gaacatgttg cttagcataa tcactgtcgc gtttcgtaac
     8101 ctcgcaggga tagtcaaaac acatgcctcg ttggcgcatc tggcgcaggc tgacgatatc
     8161 agcttcgaat ggattgttgt cgatggcggt tccaatgacg gcacccgtga gtatctggaa
     8221 aatctcaatg gtatctataa cctacgcttt gtcagcgagc cagataacgg tatctacgac
     8281 gccatgaaca aaggcattgc gatggcgcat ggcaagttcg cgttgtttct caactcaggc
     8341 gatatttttc atcaggatgc cgcaaatttt gtccgtaagt taaaagtgca aaaagataac
     8401 atgatgatca ccggcgatgc gctgctggat tttggcgacg ggcataaaat taaacgtagc
     8461 gccaaaccgg gttggtatat ttatcacagc ctgcccgcca gtcatcaggc gatatttttc
     8521 ccggtgtccg gcttgaaaaa atggcgttat gacctggaat ataaagtttc ttccgactac
     8581 gcgctggcag ccaaaatgta taaagccggt tatgcattta aaaaactcaa tggcctggtg
     8641 tctgaatttt ctatgggtgg ggtatcaacc accaataata tggaattgtg tgctgacgcc
     8701 aaaaaagtcc aacggcaaat attacatgtg cctggcttct gggcagaatt atcctggcat
     8761 ttacgccaac gtactacctc aaagacgaaa gccttatata acaaaagctg aatataaggg
     8821 aaaacgatgc aagatttaag cggtttctcg gtaccgaaag ggttccgggg tggcaacgct
     8881 attaaagtgc aattgtggtg ggcagtacag gcaacaatat ttgcctggtc gccacaagta
     8941 ttgtatcgct ggcgggcatt tttattacgt ttattcggcg caaaaatagg aaaaaacgta
     9001 gttattcgtc cgtcagtaaa aattacctat ccgtggaaat taaccttagg tgattacgcg
     9061 tgggtcggcg atgacgtcaa tttatatacc ctcggtgaaa taaccattgg cgcacattcg
     9121 gtgatatcgc aaaaaagtta tttatgcacc ggtagccacg accatgcaag tcaacatttc
     9181 accattaacg ccacgcctat tgtgattggc gagaaatgct ggctggcaac cgatgtcttt
     9241 gttgccccag gcgtcacgat cggcaacggc accgtcgtgg gtgcacgaag cagtgttttt
     9301 aaatcgcttc cggcaaatgt ggtttgccgg gggaatcccg cagtggtgat acgcgaacgc
     9361 gttgaaactg aataaattca aaaaatacag aggaataaga catgtcaaaa gtcgctctca
     9421 tcaccggtgt aaccggacaa gacggttctt acctggcaga gtttctgctg gaaaaaggtt
     9481 acgaggtgca tggtattaag cgtcgtgcat cgtcattcaa caccgagcgc gtggatcaca
     9541 tttatcagga tccgcacacc tgcaacccga aattccatct gcattatggc gacctgagtg
     9601 atacctccaa cctgacgcgt attttgcgtg aagtgcagcc ggatgaagtg tacaacctgg
     9661 gcgcaatgag ccacgttgcg gtttcttttg agtcaccgga atataccgca gacgtcgatg
     9721 cgatgggtac gctgcgcctg ctggaggcga tccgcttcct cggtctggaa aagaaaaccc
     9781 gtttctatca ggcttctact tctgaactgt acggtctggt gcaggaaatt ccgcagaaag
     9841 agaccacgcc gttctaccca cgctctccgt atgcggtcgc caaactgtac gcctactgga
     9901 tcaccgttaa ctaccgcgaa tcctacgaca tgtacgcctg taacggtatt ctcttcaacc
     9961 atgaatcccc gcgccgtggc gagactttcg ttacccgcaa aatcacccgc gcaatcgcca
    10021 acatcgccca ggggctggag tcgtgcctgt atctcggcaa tatggattcc ctgcgtgact
    10081 ggggccacgc caaagactac gtaaaaatgc agtggatgat gctgcaacag gaacaaccgg
    10141 aagatttcgt tatcgcgacc ggcgttcagt actccgtacg tcagttcgtg gaaatggcgg
    10201 cagcacagtt gggcatcaaa ctgcgctttg aaggcacggg tgttgaagag aagggcattg
    10261 tggtttccgt caccgggcat gacgcgccgg gcgttaaacc gggcgatgtg attatcgccg
    10321 ttgacccgcg ttacttccgt ccggcggaag ttgaaacgct gctcggcgac ccgaccaaag
    10381 cgcacgaaaa actgggctgg aaaccggaaa tcaccctcag agagatggtg tctgaaatgg
    10441 tggctaatga cctcgaagcg gcgaaaaaac actctctgct gaaatctcac ggctacgacg
    10501 tggcgatcgc gctggagtca taagcatgag taaacaacgc atttttattg ctggccatcg
    10561 cgggatggtc ggttccgcca tcaggcggca gctcgaacag cgcggtgatg tagagctggt
    10621 attacgcacc cgcgacgagc tgaacctgct ggacagccgc gcggtgcatg atttctttgc
    10681 cagcgaaagc attgaccagg tctatctggc ggcggcgaaa gtgggcggca ttgttgccaa
    10741 caacacgtat ccggcggatt tcatctacca gaacatgatg attgagagca acatcattca
    10801 cgccgcgcat cagaacgacg tgaacaaact gctgtttctc ggatcgtcct gcatctaccc
    10861 gaaactggca aaacagccga tggcagaaag cgagttgttg cagggcacgc tggagccgac
    10921 taacgagcct tatgctattg ccaaaatcgc cgggatcaaa ctgtgcgaat cttacaatcg
    10981 ccagtacgga cgcgattacc gctcagtcat gccgaccaac ctgtacgggc cgcatgacaa
    11041 cttccacccg agtaactccc atgtgatacc tgcattgctg cgtcgcttcc acgaggcgac
    11101 ggcacagaat gcgccggacg tggtggtatg gggcagcggt acaccgatgc gtgaattcct
    11161 gcacgtcgat gatatggcgg cggcgagcat tcatgtcatg gagctggcgc acgaagtctg
    11221 gctggaaaac acccagccga tgctgtcgca cattaacgtc ggcacgggcg ttgactgcac
    11281 tatccgcgag ctggcgcaaa ccatcgccaa agtggtgggt tacaaaggtc gggtggtttt
    11341 tgatgccagt aaaccggatg gtacaccgcg caaactgctg gatgtgacgc gcctgcatca
    11401 gcttggctgg tatcacgaaa tctcactgga agcggggctt gccagcactt accagtggtt
    11461 ccttgagaat caagaccgct ttcgggggta atgatgtttt tacgtcagga agactttgcc
    11521 acggtagtgc gctccacacc gcttgtctct ctcgacttta ttgtcgagaa cagtcgcggc
    11581 gagtttctgc ttggcaaaag aaccaaccgc ccggcgcagg gttactggtt tgtgccggga
    11641 gggcgcgtgc agaaagacga aacgctggaa gccgcatttg agcggctgac gatggcggaa
    11701 ctggggctgc gtctgccgat aacagcaggc cagttttacg gtgtctggca gcacttttat
    11761 gacgataact tctccggcac ggatttcacc actcactatg tggtgctcgg ttttcgcttc
    11821 agagtagtgg aagaagagct gttactgccg gatgagcagc atgacgatta ccgctggctg
    11881 acgccggacg cactgctcgc cagtgacaat gttcacgcta acagccgcgc ctattttctc
    11941 gctgagaagc gtgccggagt acccggatta tgaaaatact ggtctacggc attaactact
    12001 cgccggagtt aaccggcatc ggcaaataca ccggcgagat ggtggaatgg cttgcggcgc
    12061 aaggtcatga ggtgcgggtt attaccgcac cgccttacta cccgcaatgg caggtgggcg
    12121 agaactattc cgcctggcgg tacaaacgag aagagggggc cgccacggtg tggcgctgcc
    12181 cgctgtacgt gccaaaacag ccgagcaccc tgaaacgcct gttgcatctc ggcagttttg
    12241 ccgtcagcag ttttttcccg ttgatggcgc aacgtcgctg gcagccggat cgcattattg
    12301 gcgtggtgcc aacgctgttt tgcacgccgg gaatgcgcct gctggcgaaa ctctctgggg
    12361 cgcgcacggt gctccatatt caggattacg aagtggacgc catgctgggg ctgggccttg
    12421 ccggaaaagg taaaggcggc aaagtggcac agctggcaac ggcgttcgaa cgtagcggac
    12481 tgcataacgt cgataacgtc tccacgattt cgcgttcgat gatgaataaa gctatcgaaa
    12541 aaggcgtggc ggcggaaaac gtcatcttct tccccaactg gtcggaaatc gcccgttttc
    12601 agcatgttgc aaatgccgat gttgatgccc ttcgtaacca gcttggcctg ccggataaca
    12661 aaaaaatcat tctttactcc ggcaatattg gtgaaaagca ggggctggaa aacgttattg
    12721 aagctgccga tcgcctgcgc gatgaaccgc tgatttttgc cattgtcggg cagggcggcg
    12781 gcaaagcgcg gctggaaaaa atggcgcagc agcgtggact gcgcaacatg caattttttc
    12841 cgctgcaatc gtatgacgct ttacccgcac tgctgaagat gggcgattgc catctggtgg
    12901 tgcaaaaacg cggcgcggca gatgccgtat tgccgtcgaa actgaccaat attctggcgg
    12961 taggcggtaa cgcggtgatt accgcagaag cccacaccga actgggacag ctttgcgaaa
    13021 cctttccggg cattgcggtt tgcgtggaac cggaatcggt cgaggcgctg gtggcgggga
    13081 ttcgtcaggc gctcctgctg cccaaacaca acacggtggc acgtgaatat gccgaacgca
    13141 cgctcgataa agagaacgtg ttacgtcaat ttataaatga tattcgggga taattatggc
    13201 gcagtcgaaa ctctatccag ttgtgatggc aggtggctcc ggtagccgct tatggccgct
    13261 ttcccgcgta ctttacccca agcagttttt atgcctgaaa ggcgatctca ccatgctgca
    13321 aaccaccatc tgccgcctga acggcgtgga gtgcgaaagc ccggtggtga tttgcaatga
    13381 gcagcaccgc tttattgtcg cggaacagct gcgtcaactg aacaaactca ccgagaatat
    13441 tattctcgaa ccggcagggc gtaacactgc acctgccatt gcgctggcgg cgctggcggc
    13501 aaaacgtcat agcccggaga gcgacccatt aatgctggtg ctggcggcgg atcatgtgat
    13561 tgccgatgaa gacgcttttc gcaccgccgt gcgtaatgcc atgccgtatg ccgaagcggg
    13621 caagctggtg accttcggca ttgtgccgga tctacctgaa accggatatg gctatattcg
    13681 tcgcggtgaa gtgtctgcgg gtgagcagga tacggtggcc tttgaagtgg cgcagtttgt
    13741 cgaaaaaccg aatctggaaa ccgcgcaggc ctatgtggca agcggcgaat attactggaa
    13801 cagcggtatg tttctgttcc gcgccggacg ctatctcgaa gaactgaaaa aatatcgccc
    13861 ggatattctc gacgcctgtg aaaaagcgat gagcgccatc gatccggatc tcgattttat
    13921 tcgtgtggat gaagaagcgt ttctcgcctg cccggaagag tcggtggatt acgcagtcat
    13981 ggaacgtacg gcagatgctg ttgtggtgcc gatggatgcg ggctggagcg atgtcggttc
    14041 ctggtcctct ttatgggaga tcagcgccca caccgccgag ggcaacgttt gccacggcga
    14101 tgtgattaat cacaaaactg aaaacagcta tgtgtatgcc gaatctggcc tggtcaccac
    14161 cgtcggggtg aaagatctgg tggtggtgca gaccaaagat gcggtgctga ttgccgaccg
    14221 taacgcggtg caggatgtga aaaaagtggt cgagcagatc aaagccgatg gtcgccatga
    14281 gcatcgggtg catcgcgaag tgtatcgtcc gtggggcaaa tatgactcta tcgacgcggg
    14341 cgaccgctac caggtgaaac gcatcaccgt gaaaccgggc gaaggcttgt cagtacagat
    14401 gcaccatcac cgcgcggaac actgggtagt ggtcgcggga acggcaaaag tcactatcga
    14461 cggtgatatc aaactccttg gtgaaaacga gtccatttat attccgctgg gggcgacgca
    14521 ttgcctggaa aacccgggga aaattccgct cgatttaatt gaagtgcgct ccggctctta
    14581 tcttgaagag gatgatgtgg tgcgcttcgc ggatcgctac ggacgggtgt aaacgtcgca
    14641 tcaggcgctg tttgtcggat gcggcgtgaa cgccttatcc gacctacggt tcgggtttgt
    14701 aggcctgata agacgcggca gcgtcgcatc aggcaatgaa tgcgaaaccg cgatgtaaat
    14761 aacgacaaaa acaaaattgg ccgtttcggt cagggccaac tattgcctga aaaagggtaa
    14821 cgatatgaaa aaattaacct gctttaaagc ctatgatatt cgcgggaaat taggcgaaga
    14881 actgaatgaa gatatcgcct ggcgcattgg tcgcgcctat ggcgaatttc tcaaaccgaa
    14941 aaccattgtg ttaggtggtg atgcccgcct caccagcgaa accttaaaac tggcgctggc
    15001 gaaaggttta caggatgcgg gcgtcgatgt gctggatatt ggtatgtccg gcaccgaaga
    15061 aatctatttc gccacgttcc atctcggcgt ggatggcggc attgaagtta ccgccagcca
    15121 taacccgatg gattacaacg gtatgaaact cgtgcgcgag ggggcccgcc cgatcagcgg
    15181 ggataccgga ctgcgcgacg tccagcgtct ggcagaagcc aacgactttc ctcccgttga
    15241 tgaaaccaaa cgcggtcgct atcagcaaat caatctgcgt gacgcttacg ttgatcacct
    15301 gttcggttat atcaacgtca aaaacctcac gccgctcaag ctggtgatca actccgggaa
    15361 cggcgcagcg ggtccggtgg tggacgccat tgaagcccgc tttaaagccc tcggcgcacc
    15421 agtggaattg atcaaagtgc acaacacgcc ggacggcaat ttccccaacg gaattcctaa
    15481 cccgctgctg ccggaatgcc gcgacgacac ccgcaatgcg gtcatcaaac acggcgcgga
    15541 tatgggcatt gcctttgatg gcgattttga ccgctgtttt ctgtttgacg aaaaagggca
    15601 gtttatcgag ggctactaca ttgtcggcct gctggcagaa gcgttcctcg aaaataatcc
    15661 cggcgcgaag atcatccacg atccgcgtct ctcctggaac accgttgatg tggtgactgc
    15721 cgcaggcggc accccggtaa tgtcgaaaac cggacacgcc tttattaaag aacggatgcg
    15781 caaggaagac gccatctacg gtggcgaaat gagcgcccac cattacttcc gtgatttcgc
    15841 ttactgcgac agcggcatga tcccgtggct gctggtcgcc gaactggtgt gcctgaaagg
    15901 gaaaacgctg ggcgaactgg tgcgtgaccg gatggcagca tttccggcaa gcggtgagat
    15961 caacagcaaa ctggcggacc ctgttgaggc gattaaccgc gtcgaacagc attttagccg
    16021 cgaggcgctg gcggtggatc gcaccgatgg catcagcatg acctttgccg actggcgctt
    16081 taacctgcgc acctccaaca ccgaaccggt ggtgcgcctg aatgttgaat cgcgcggtga
    16141 tgtgccgctg atggaagcgc gaacgcgaac tctgctgacg ttgctgaacg agtaatgtcg
    16201 gatcttccct taccccactg cgggtaaggg gctaataaca ggaacaacga tgacaaatct
    16261 aaaaaagcgc gagcgagcga aaaccaatgc atcgttaatc tctatggtgc aacgcttttc
    16321 agatatcacc atcatgtttg ccggactatg gctggtttgc gaagtcagcg gactgtcatt
    16381 cctctacatg cacctgttgg tggcgctgat tacgctggtg gtgttccaga tgctgggcgg
    16441 catcaccgat ttttatcgct catggcgcgg tgttcgggca gcgacagaat ttgccctgct
    16501 gctacaaaac tggaccttaa gcgtgatttt cagcgccggg ctggtggcgt tcaacaatga
    16561 tttcgacacg caactgaaaa tctggctggc gtggtatggg ctgaccagta tcggactggt
    16621 ggtttgccgt tcgtgtattc gcattggggc gggctggctg cgtaatcatg gctataacaa
    16681 gcgcatggtc gcggtggcgg gggatttagc cgccggacaa atgctgatgg agagcttccg
    16741 taatcagccg tggttagggt ttgaagtggt gggcgtttac cacgacccga aaccgggtgg
    16801 cgtttctaac gactgggcgg gcaatctgca acagctggtc gaggatgcga aagcgggcaa
    16861 gattcataac gtctatatcg cgatgcaaat gtgcgacggc gcacgcgtga aaaaactggt
    16921 ccatcaattg gcggacacca cctgttcggt gctgctgatc cccgatgtct ttaccttcaa
    16981 cattctccat tcccgtatcg aagagatgaa cggcgtaccg gtggtgccgc tgtatgacac
    17041 gccgctttcc ggggttaacc gcctgctcaa acgtgcggaa gatattgtgc tggcgacgct
    17101 tatcctgctg ctgatctccc cggtgctgtg ctgcattgcg ctggcggtga aactgagctc
    17161 accggggccg gttattttcc gccagactcg ctacggcatg gacggcaagc cgatcaaagt
    17221 gtggaagttc cgttccatga aagtgatgga gaacgacaaa gtggtgaccc aggcgacgca
    17281 gaacgatccg cgcgtcacca aagtggggaa ctttctgcgc cgcacctcgc tggatgaatt
    17341 gccgcagttt atcaatgtgc tgactggcgg gatgtcgatt gtcgggccac gtccgcacgc
    17401 ggtggcgcat aacgaacagt atcgccagct cattgaaggc tacatgctgc gccataaagt
    17461 gaaaccgggc attaccggct gggcgcagat taacggctgg cgcggcgaaa ccgacacact
    17521 ggagaaaatg gaaaaacgcg tcgagttcga ccttgagtac atccgcgaat ggagcgtctg
    17581 gttcgatatc aaaatcgttt tcctgacggt attcaagggc ttcgttaaca aagcggcata
    17641 ttgatatgaa cttacgtgaa aaaaccatca gcggcgcgaa gtggtcggcg attgccacgg
    17701 tgatcatcat cggcctcggg ctggtgcaga tgaccgtgct ggcgcggatt atcgacaacc
    17761 accagttcgg tttgcttacc gtgtcgctgg tgatcatcgc gctggcagat acgctttctg
    17821 acttcggcat cgctaactcg attattcagc gaaaagaaat cagccacctt gaactcacca
    17881 cgctgtactg gctgaacgtc gggctgggga tcgtggtgtg cgtggcggtg tttttgttga
    17941 gtgataccat tggcgacgtg ctgaataacc cggacctggc accgttgatt aaaacattgt
    18001 cgctggcgtt tgtggtgatc ccccacgggc agcagttccg cgcgttgatg cagaaggagc
    18061 tggagttcaa caaaatcggc atgatcgaaa ccagtgcggt gctggcgggc ttcactttta
    18121 cggtggttag cgcccatttc tggccgctgg cgatgaccgc gatcctcggt tatctggtca
    18181 atagtgcggt aagaacgctg ctgtttggct actttggccg caaaatttat cgccccggtc
    18241 tgcatttctc gctggcgtcg gtggcaccaa acttacgttt tggtgcctgg ctgacggcgg
    18301 acagcatcat caactatctc aataccaacc tttcaacact cgtgctggcg cgtattctcg
    18361 gcgcgggcgt ggcaggggga tacaacctgg cgtacaacgt ggccgttgtg ccgccgatga
    18421 aactgaaccc catcatcacg cgcgtgttgt ttccggcatt tgccaaaatt caggacgaca
    18481 ccgaaaagct gcgcgtcaac ttctacaagc tgctgtcggt ggtggggatt atcaactttc
    18541 cggcgctgct ggggctgata gtggtgtcga ataactttgt accgctggta tttggtgaga
    18601 agtggaacag cattattccg gtgctgcaat tgctgtgtgt ggtggggctg ctgcgctcag
    18661 tggggaaccc gattggttcg ctgctgatgg cgaaagcgcg ggtcgatatc agctttaaat
    18721 tcaacgtatt caaaaccttt ctgtttattc cggcgattgt tattggtggg cagatggcgg
    18781 gcgcaatcgg cgtcacgctt ggcttcctgc tggtgcagat tatcaacacc attctgagct
    18841 atttcgtgat gattaaaccg gtgctcggtt ccagttatcg ccagtacatc ctgagtttgt
    18901 ggctgccgtt ttatctctcg ctgccgacgc tggtggtcag ttatgcgctg ggcattgtgc
    18961 tgaaagggca actggcgctg gggatgctgc tggcggtgca aatagccacg ggcgtgctgg
    19021 cgtttgtggt gatgattgtg ctgtcgcgcc atccgctggt ggtggaagtg aagcgtcagt
    19081 tttgtcgcag cgaaaaaatg aaaatgcttt tacgggcggg gtgaatggtt gttttcggtt
    19141 ccggcaacgg ttgccggatg cggcgtaaaa cgccttatcc gtcctggggg gctgggcttg
    19201 gatcttgtag gcctgataag acgcgccagc gtcgcatcag gcactgaatg ccggatgcgg
    19261 cgtgaacgcc ttatccggcc tacggttctg tgctcggatc ttgtaggcct gataagacgc
    19321 gtcagcgtcg catcaggcac tgaatgccgg atgcggcgta aaacgcctta tccgtcctac
    19381 ggctctgtgc tcgggtcttg taggcctgat aagacgcgtc agcgtcgcat caggcactga
    19441 atgccggaag cggcgtgaac gccttatccg tcctacggtt ctgggcttgg atcttgtagg
    19501 cctgataaga ggcgtcagcg tcgcatcagg tactgaatgc cggatgcggc gtgaacgcct
    19561 tatccgtcct acggttctgg gcttggatct tgtaggcctg ataagacgcg tcagcgtcgc
    19621 atcaggcact gaatgccgga tgcggcgtga acgccttatc cgtcctacgg ttctgtgctc
    19681 ggatcttgta ggcctgataa gacgcgttag cgtcgcatca ggcaaaaaac ggcaacgaac
    19741 acaacaaaca cagcctcacc tggcatcaaa tttatgagga aaaaatgaaa ttacttattc
    19801 tgggcaacca cacttgcggc aatcgtggcg atagcgccat cctgcgtggc ttacttgatg
    19861 ccatcaatat cctcaatcca catgccgaag tggacgtgat gagccgctat ccggtcagtt
    19921 cttcctggct gctcaaccgc ccggtaatgg gcgatccgct gttcctgcaa atgaaacaac
    19981 acaacagcgc ggcgggcgtt gtcgggcgtg ttaaaaaagt cctccgtcgc cgctaccagc
    20041 accaggtatt gctctcacgc gtcaccgaca ctggcaagct gcgtaatatc gccatcgccc
    20101 agggattcac tgacttcgtg cgactgctgt caggttacga cgccattatt caggtcggcg
    20161 gatcgttttt tgtcgatctc tacggcgtgc cgcagtttga acatgcgctt tgcactttca
    20221 tggcgaaaaa gccgctgttt atgattggtc acagcgtcgg ccccttccag gatgagcaat
    20281 ttaaccaact ggcgaactat gtctttggtc actgcgacgc gctgatcctg cgcgaatcgg
    20341 tcagccttga tttgatgaaa cgcagcaata tcaccactgc aaaagtggaa catggcgtcg
    20401 ataccgcgtg gctggtcgat caccacattg aagacttcac cgccagctat gctgttcaac
    20461 actggctgga tgttgccgca caacagaaaa cggtggcaat taccctgcgc gaactggcac
    20521 cgttcgacaa acgtctcggc accactcaac aagcgtatga aaaagccttt gccggggtgg
    20581 tcaatcgcat tctcgatgaa gggtatcagg tgattgcgct ctccacctgt acgggcatcg
    20641 acagttataa caaagacgac cgcatggtgg cgctcaatct gcgccagcac atcagcgatc
    20701 ctgcccgtta tcacgtagtg atggatgaac tcaacgatct ggaaatgggc aaaattctgg
    20761 gggcctgtga acttaccgtc ggtacgcgcc tgcactctgc tattatctcg atgaactttg
    20821 ccactccggc gattgccatc aactacgaac ataaatccgc cgggatcatg caacaactgg
    20881 ggctaccaga aatggcgatt gatatccgtc atttattaga cggcagcctg caagcgatgg
    20941 ttgcggatac cttaggccag cttccggcac tgaacacgcg acttaacgaa gccgtcagtc
    21001 gcgaacgtca gacgggaatg cagatggtgc aatctgtgct tgaacgcatc ggggaggtga
    21061 aatgaaggtc ggcttctttt tactgaaatt tccgttgtcg tcggaaacct tcgttcttaa
    21121 ccaaatcact gcgtttattg atatgggatt tgaggtggag attatcgcgc tgcaaaaagg
    21181 cgacacacaa aacacccacg cggcatggac gaaatacaac cttgctgcca gaacccgctg
    21241 gttacaggac gaacctacgg gcaaagtggc gaaactgcgc caccgcgcca gccagacctt
    21301 atgcggcatt catcgtaaaa atacctggca ggcgctcaac ctcaaacgct atggcgccga
    21361 gtcgcggaac ctgattttgt ctgctatttg cggtcaggtc gcaacaccgt ttcatgccga
    21421 tgtctttatc gctcactttg gtccggcagg ggtaaccgca gcaaaactcc gcgaactggg
    21481 tgtcattcgc ggcaaaattg ccactatctt ccacggtatt gatatctcca gtcgggaggt
    21541 gctcaaccat tacactcccg aatatcagca actgttccgc cgtggcgacc tgatgttacc
    21601 gataagcgat ctgtgggccg gaaggctgca aaaaatgggc tgcccgaggg aaaaaatcgc
    21661 cgtatcgcgt atgggcgtgg acatgacgcg ctttagcccg cgtccggtga aagcgcctgc
    21721 aacgccgctg gaaatcattt ccgtcgcacg cttaaccgag aaaaaaggcc tgcatgtggc
    21781 gatcgaagcc tgccgtcagt tgaaagagca gggcgtggca tttcgctatc gcatcctcgg
    21841 cattggcccg tgggaacgac gcctgcgcac cctcatcgaa caatatcaac tggaagatgt
    21901 gatagagatg ccgggcttta aaccgagcca tgaagtgaag gcgatgctcg acgacgcgga
    21961 tgtcttcctg ttgccatcgg ttacaggtgc ggacggcgat atggaaggta ttccggtggc
    22021 actaatggaa gcgatggcgg tcggcattcc ggtggtttct actctgcata gcggaatacc
    22081 ggaactggtg gaggccgata aatccggctg gctggtgcct gagaacgatg ctcgcgcact
    22141 ggcgcaacga ctggcggcgt ttagccaact ggacaccgac gaactggctc cggttatcaa
    22201 acgtgctcgc gaaaaagtcg aacacgattt taaccagcag gtgatcaatc gagaactcgc
    22261 cagcttgctg caggctttat agaggagaac gtatgccatt taaaaaactc tcccgacgca
    22321 ccttcctgac ggcaagctcg gcgcttgcct tcctccatac ccccttcgcc cgcgcacttc
    22381 cagcccgaca aagcgttaat atcaacgatt acaatccgca cgactggatc gcctcattta
    22441 aacaagcctt cagtgaaggg caaacggtcg tcgtgcctgc cggattggtt tgtgacaata
    22501 tcaacaccgg catcttcatt cctcctggaa aaaccttaca catccttgga agcctgcgcg
    22561 gcaacggcag agggcgattt gtcttacagg acggcagcca ggtgacaggg gaggagggcg
    22621 gcagtatgca taacatcacc ctggatgtgc gcggttctga ctgcatcatc aaagggttgg
    22681 cgatgagcgg ttttggcccg gtaacgcaga tttatatcgg cggcaaaaat aaacgcgtca
    22741 tgcgcaactt gaccatcgat aacctgaccg tcagccacgc taattacgcc attttacgcc
    22801 agggatttca taaccagatt atcggtgcca acatcaccaa ctgtaagttc agcgacttac
    22861 agggcgacgc cattgaatgg aatgtggcga ttaacgaccg cgatattttg atatccgacc
    22921 acgttattga gcgcatcaac tgtaccaacg gcaaaatcaa ctggggcatc ggcataggcc
    22981 ttgcgggaag cacttacgat aacaactatc cggaagacca ggcagtgaaa aactttgtcg
    23041 tggcgaatat cacgggatca gattgtcgac agttgataca tgttgaaaat ggtaaacatt
    23101 ttgttattcg taatatcaaa gcccgcaata tcacgccgga tttcagtaag aaagcaggta
    23161 ttgataacgc cacggtcgct atttacggtt gtgacaattt cgtgattgat aatattgaaa
    23221 tgattaatag cgccgggatg ttaattggct atggggtaat taaaggcaaa tatctctcga
    23281 taccgcaaaa tttccgagtg aataatattc aactggataa cactcacctt gcttataaat
    23341 tgcgcggcat ccaaatctcc gccgggaatg ctgtctcctt tgtggcgctg actaacattg
    23401 agatgaagcg tgcctcgctg gagcttcaca ataaaccgca acatcttttt atgcgaaata
    23461 tcaaagtgat gcaggaatcc tctgttggac ccgcattgag catgaacttc gacatgcgca
    23521 aagacgttcg cggcgtcttt atggcgaaaa aagaaacact gctgtctctt gcaaatgttc
    23581 atgcgatgaa tgaaagaggg caaagctccg tcgatatcga cagggttaat caccatattg
    23641 ttaatgtgga aaagattaac tttagattgc cagaacggag ggagtagatt tgcgaccatt
    23701 cataaactta ctggatataa tgttctaaaa gattataatc ctcacactaa ctgttaaagc
    23761 tggggctggg cagcattgac tactcaggga aggtactcat ctttatcttg tgtttcttac
    23821 gtaacaacca gactaaagtg atttatgaac gataacgttt tgctcatagg agcttccgga
    23881 ttcgtaggaa cccgactact tgaaacggca attgctgact ttaatatcaa gaacctggac
    23941 aaacagcaga gccactttta tccagaaatc acacagattg gcgatgttcg tgatcaacag
    24001 gcactcgacc aggcgttagc tggttttgac actgttgtgc tactggcagc ggaacaccgc
    24061 gatgacgtca gccctacttc tctctattat gatgtcaacg ttcagggtac ccgcaatgtg
    24121 ctgacggcca tggaaaaaaa tggcgttaaa aatatcatct ttaccagttc cgttgctgtt
    24181 tatggtttga acaaacacaa ccctgacgaa aaccatccac acgatccttt caaccactac
    24241 ggcaaaagta agtggcaggc agaggaagtg ctgcgtgaat ggtataacaa agcaccaaca
    24301 gaacgttcat taaccatcat ccgtcctacc gttatcttcg gtgaacgcaa ccgcggtaac
    24361 gtctataact tgctgaaaca gatcgctggc ggcaagttta tgatggtggg cgcagggact
    24421 aactataagt ccatggctta tgttggaaac attgttgagt ttatcaagta caaactgaag
    24481 aatgttgccg caggttatga ggtttataat tacgttgata agccagatct gaacatgaac
    24541 cagttggttg ctgaagttga acaaagtctg aacaaaaaga tcccttctat gcacttgcct
    24601 tacccactag gaatgttggg tggatattgc tttgatatcc tgagcaaaat tacgggcaaa
    24661 aaatacgctg tcagctctgt gcgcgtgaaa aaattctgcg caacaacaca gtttgacgca
    24721 acgaaagtgc attcttcagg ttttgtggca ccgtatacgc tgtcgcaagg tctggatcga
    24781 acactgcagt atgaattcgt tcatgccaaa aaagacgaca taacgtttgt ttctgagtaa
    24841 tgcagacgag tcagagaaaa aaggaccagg ttggactacg attattcgga aagaggtact
    24901 gattaaatta tcagtatgcg gaattccgag taatccgagt aacccagatg ctgatggtgt
    24961 gtagtgttat tctaaggttt aagcaaagtt atgcggaact aagctttggt atatagccat
    25021 aacgtttggc taacctatgg caataattta cgcactatca gccacttaga ggtaggaaaa
    25081 gaatgacgaa tttaaaagca gttataccgg tagcaggtct tgggatgcat atgttgcctg
    25141 ccactaaggc gattcccaaa gagatgctac cgatcgtcga caagccaatg attcagtaca
    25201 ttgttgacga gattgtggct gcagggatca aagaaatcct cctggtaact cacgcgtcca
    25261 agaacgcggt cgaaaaccac ttcgacacct cttatgaatt agaatctctc cttgagcagc
    25321 gcgtgaagcg tcaactactg gcggaagtgc aatctatctg cccgccgggc gtgactatta
    25381 tgaacgtgcg tcagggcgaa cctttaggtt taggtcactc cattttgtgt gcacgccccg
    25441 ccattggtga caacccattt gtcgtggtgc tgccggacgt tgttatcgat gatgccagcg
    25501 ccgacccgct gcgttacaac cttgccgcta tgattgcgcg cttcaacgaa acgggccgca
    25561 gccaggtgct ggcaaaacgt atgccgggtg acctctctga atactccgtc atccagacca
    25621 aagaaccgct ggatcgtgaa ggcaaagtca gccgcattgt tgaattcatc gaaaaaccgg
    25681 atcagccgca gacgctggac tcagacatca tggccgttgg tcgctatgtg ctttctgccg
    25741 atatttggcc ggaactggaa cgcactcagc ctggtgcatg ggggcgtatt cagctgactg
    25801 atgctattgc cgaactggcg aaaaaacagt ccgttgatgc catgctgatg actggtgaca
    25861 gctacgactg tggtaaaaaa atgggctata tgcaggcgtt tgtgaagtat ggactgcgca
    25921 acctgaaaga aggggcgaag ttccgtaaag gtattgagaa gctgttaagc gaataatgaa
    25981 aatctgaccg gatgtaacgg ttgataagaa aattataacg gcagtgaaga ttcgtggcga
    26041 aagtaatttg ttgcgaatat tcctgccgtt gttttatata aacaatcaga ataacaaaga
    26101 gttagcaata ggattttcgt caaagttttc caggattttc cttgtttcca gagcggattg
    26161 gtaagacaat tagcgtttga atttttcgag ttaagcgcga gtgggtaacg ctcgtcacat
    26221 cgtaggcatg catgcagtgc tctggtagct gtaaagccag gggcggtagc gtgcattaat
    26281 acctctatta atcaaactaa gagccgctta tttcacagca tgctctgaag taatatggaa
    26341 taaaagagtg aagatacttg ttactggtgg cgcaggattt attggttctg ctgtagttcg
    26401 tcacattata aataatacgc aggatagtgt tgttaatgtc gataaattaa cgtacgccgg
    26461 aaacctggaa tcacttgctg atgtttctga ttctgaacgc tatatttttg aacatgcgga
    26521 tatttgcgat gctgctgcaa tggcgcggat ttttgctcag catcagccgg atgcagtgat
    26581 gcacctggct gctgaaagcc atgtggatcg ttcaattaca ggccctgcgg catttattga
    26641 aaccaatatt gttggtactt atgtcctttt ggaagccgct cgcaattatt ggtctgctct
    26701 tgatggcgac aagaaaaata gcttccgttt tcatcatatt tctactgacg aagtctatgg
    26761 tgatttgcct catcctgacg aagtaaataa taaagaacaa ttacccctct ttactgagac
    26821 gacagcttac gcgcctagta gtccttattc cgcatcaaaa gcatccagcg atcatttagt
    26881 ccgtgcgtgg aaacgtacct atggtttacc gaccattgtg actaattgtt cgaataacta
    26941 cggtccttat cactttccgg aaaaattgat tccactagta attcttaatg ctctggaagg
    27001 taaggcatta cctatttatg gcaaagggga tcaaattcgc gactggctgt atgttgaaga
    27061 tcatgcacgt gcgttatata ccgtcgtaac cgaaggtaaa gcgggtgaaa cttataacat
    27121 tggtggacac aacgaaaaga aaaacatcga tgtagtgctc actatttgtg atttgttgga
    27181 tgagattgta ccgaaagaga aatcttaccg cgagcaaatt acttatgttg ccgatcgtcc
    27241 gggacacgat cgccgttatg ccattgatgc tgagaagatt ggtcgcgaat tgggatggaa
    27301 accacaggaa acgtttgaga gcgggattcg gaagacagtg gaatggtacc tgtccaatac
    27361 aaaatgggtt gataatgtga aaagtggtgc ctatcaatcg tggattgaac agaactatga
    27421 gggccgccag taatgaatat tctccttttc ggcaaaacag ggcaggtagg ttgggaacta
    27481 cagcgtgctc tggcaccttt gggtaatttg attgctcttg atgttcactc cactgattat
    27541 tgtggtgatt ttagtaatcc tgaaggtgtg gctgaaaccg tcaaaaaaat tcgccctgat
    27601 gttattgtta atgctgcggc tcataccgca gtagataagg ctgagtcaga acccgaattt
    27661 gcacaattac tcaatgcgac cagcgttgaa tcaattgcaa aagcggctaa tgaagttggg
    27721 gcctgggtaa ttcattactc aactgactac gtatttccgg gaaccggtga aataccatgg
    27781 ctggagacgg atgcaaccgc accgctaaat gtttacggtg aaaccaagtt agctggagaa
    27841 aaagcattac aagagcagtg tgcgaagcac cttattttcc gtaccagctg ggtatatgca
    27901 ggtaaaggaa acaattttgc caagacaatg ttacgtctgg caaaagagcg tgaagaatta
    27961 gccgttatta atgatcagtt tggtgcgcca actggcgcag agttgctggc tgattgtacg
    28021 gcacatgcca ttcgtgtggc actgaataaa ccggaagtcg caggtttgta ccatctggta
    28081 gccagtggta ccacaacctg gcacgattat gctgcgctgg tttttgaaga ggcgcgcaaa
    28141 gcaggcatac cccttgcact caacaagctc aacgcagtag caacaacagc ctatcctaca
    28201 ccagctcgtc gtccacataa ctctcgcctt aatacagaaa aatttcagca gaattttgcg
    28261 cttgttttgc ctgactggca ggttggcgtg aaacgaatgc tcaacgaatt atttacgact
    28321 acagtaattt aatagttttt gcatcttgtt cgtgatgatg gagcaagatg aattaaaagg
    28381 aatgatgaaa tgaaaacgcg taaaggtatt attttagcgg gtggctctgg tactcgtctt
    28441 tatcctgtga ctatggctgt cagtaaacag ctgttaccta tttatgataa accgatgatc
    28501 tattacccgc tttctacact gatgttagcg ggtattcgcg atattctgat tatcagtaca
    28561 ccacaggata ctcctcgttt tcaccaactg ctgggtgacg ggggccagtg ggggctaaat
    28621 cttcagtaca aagtgcaacc gactccagat gggcttgcgc aggcgtttat tatcggtgaa
    28681 gagtttatcg gtggtgatga ttgtgctctg gttctcggtg ataatatctt ttacggtcat
    28741 gatctgccga agttaatgga tgtcgctgtt aacaaagaaa gtggtgcaac agtatttgcc
    28801 tatcatgtta atgatcctga acgctacggt gtcgttgagt ttgataaaaa aggtacggca
    28861 attagcctgg aagaaaaacc gttacaacca aaaagtaatt atgcggtaac cgggctttat
    28921 ttctatgata acgacgttgt cgaaatggcg aaaaacctta agccttctgc ccgtggtgaa
    28981 ctggaaatta ccgatattaa ccgtatttat atggaacagg gggggttatc cgttgccatg
    29041 atgggacgtg gttatgcatg gctggacacg ggtacacatc aaagtcttat tgaagcaagc
    29101 aacttcattg caacaattga agagcgtcag gggttaaaag tttcctgtcc ggaagaaatt
    29161 gcttaccgta aagggtttat tgatgctgag caggtgaaag tattagctga accgctgaaa
    29221 aaaaatgctt atggtcagta tctgctgaga atgattaaag gttattaata aaatgaacgt
    29281 aattaaaaca gaaattccag aagtcttgat ttttgagcct aaaaaatttg aggactccag
    29341 ggggtatttt tttgaaagct ttaatcaaag gctttttgaa gatgccgtag gaaaagaaat
    29401 tctattcgtc caagataatc aatcat
//
LOCUS       DAZFLD010000035        29371 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.035, whole genome shotgun sequence.
ACCESSION   DAZFLD010000035 DAZFLD010000000
VERSION     DAZFLD010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29371
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.035"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(182..448)
                     /locus_tag="ACHKBL_001324"
                     /pseudo
     CDS             complement(182..448)
                     /locus_tag="ACHKBL_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226947.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(724..2040)
                     /gene="hipA"
                     /locus_tag="ACHKBL_001325"
     CDS             complement(724..2040)
                     /gene="hipA"
                     /locus_tag="ACHKBL_001325"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system serine/threonine
                     protein kinase toxin HipA"
                     /protein_id="HFO5818217.1"
                     /translation="MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLASRYARPLSLS
                     LPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYHAKSRQPFDLLSEIGRDSVGAVT
                     LLPENETITRPIMAWEQLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
                     LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVP
                     DAEIIKAGNVRALAVERFDRRWNAERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIA
                     RIMAFLMGSSEALKDRYDFMKFQVFQWLIGATDGHAKNFSVFIQAGGSYRLTPFYDII
                     SAFPVLGGAGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLKFPQVQMHEIL
                     SDFARMIPAALDNVKTSLPTDFPENVVTAVETNVLRLHRRLSREWY"
     gene            complement(2040..2306)
                     /gene="hipB"
                     /locus_tag="ACHKBL_001326"
     CDS             complement(2040..2306)
                     /gene="hipB"
                     /locus_tag="ACHKBL_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HipB"
                     /protein_id="HFO5818218.1"
                     /translation="MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISN
                     FENNPDNTTLTTFFKILQSLELSMTLCDTKNASPESTEQQDLEW"
     gene            complement(2499..4388)
                     /locus_tag="ACHKBL_001327"
                     /pseudo
     CDS             complement(2499..4388)
                     /locus_tag="ACHKBL_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244610.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter barrel domain-containing
                     lipoprotein"
     gene            complement(4538..6130)
                     /gene="lsrK"
                     /locus_tag="ACHKBL_001328"
     CDS             complement(4538..6130)
                     /gene="lsrK"
                     /locus_tag="ACHKBL_001328"
                     /EC_number="2.7.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416028.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071518 - autoinducer-2 kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer-2 kinase"
                     /protein_id="HFO5818219.1"
                     /translation="MARLFTPSESKYYLMALDAGTGSIRAVIFDPEGNQIAVGQAEWR
                     HLAVPDVPGSMEFDLNKNWQLACECMRQALHNAGIAPEYIAAVSACSMREGIVLYNNE
                     GTPIWACANVDARAAREVNELKELHNNTFENKVYRATGQTLALSAIPRLLWLAHHRSD
                     IYRQASTITMISDWLAYMLSGELAVDPSNAGTTGLIDLTTRDWKPALLDMAGLRADIL
                     SPVKETGTLLGVVSSQAAELCGLKAGTPVVVGGGDVQLGCLGLGVVRPAQTAVLGGTF
                     WQQVVNLAAPVTDPEMNVRVNPHVIPGMVQAESISFFTGLTMRWFRDAFCAEEKLIAE
                     RLGIDTYTLLEEMASRVPPGSWGVMPIFSDRMRFKTWYHAAPSFINLSIDPDKCNKAT
                     LFRALEENAAIVSACNLQQIADFSNIHPTSLVFAGGGSKGKLWSQILADVSGLPVNIP
                     VVKEATALGCAIAAGVGAGIFSSMAETGERLVRWERTHTPDPEKHELYQDSRDKWQAV
                     YQDQLGLVDHGLTTSLWKAPGL"
     gene            complement(6209..7162)
                     /gene="lsrR"
                     /locus_tag="ACHKBL_001329"
     CDS             complement(6209..7162)
                     /gene="lsrR"
                     /locus_tag="ACHKBL_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LsrR"
                     /protein_id="HFO5818220.1"
                     /translation="MTINDSVISEQGMCEEEQVARIAWFYYHDGLTQSEISDRLGLTR
                     LKVSRLLEKGHQSGIIRVQINSRFEGCLEYETQLRRQFSLQHVRVIPGLADADVGGRL
                     GIGAAHMLMSLLQPQQMLAIGFGEATMNTLQRLSGFISSQQIRLVTLSGGVGSYMTGI
                     GQLNAACSVNIIPAPLRASSADIARTLKNENCVKDVLLAAQAADVAIVGIGAVSQQDD
                     ATIIRSGYISQGEQLMIGRKGAVGDILGYFFDAKGDVVTDIKIHNELIGLPLSALKTI
                     PVRVGVAGGENKAEAIAAAMKGGYINALVTDQDTAAAILRS"
     gene            7411..8946
                     /gene="lsrA"
                     /locus_tag="ACHKBL_001330"
     CDS             7411..8946
                     /gene="lsrA"
                     /locus_tag="ACHKBL_001330"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter ATP-binding
                     protein LsrA"
                     /protein_id="HFO5818221.1"
                     /translation="MQTSDTRALPLLCARSVYKQYSGVNVLKGIDFTLHQGEVHALLG
                     GNGAGKSTLMKIIAGITPADSGTLEIGGNNYTRLTPVHAHQLGIYLVPQEPLLFPSLS
                     IKENILFGLAKKQLSIQKMNNLLVALGCQFDLHSLAGSLDVADRQMVEILRGLMRDSR
                     ILILDEPTASLTPAETERLFRRLQELLATGVGIVFISHKLPEIRQIADRISVMRDGTI
                     ALSGKTSELSTDDIIQAITPALREKSLSASQKLWLELPGNRPQHAAGTPVLTLENLTG
                     EGFRNVSLTLNAGEILGLAGLVGAGRTELAETLYGLRTLRGGRIMLNGKEINRLSTGD
                     RLLRGLVYLPEDRQSSGLNLDASLAWNVCALTHNLRGFWAKTAKDNATLERYRRALNI
                     KFNQPEQAARTLSGGNQQKILIAKCLEASPQVLIVDEPTRGVDVSARNDIYQLLRSIA
                     AQNVAVLLISSDLEEIELMADRVYVMHQGEIAHSALTGRDINVETIMRVAFGDSQRQE
                     ASC"
     gene            8940..9968
                     /gene="lsrC"
                     /locus_tag="ACHKBL_001331"
     CDS             8940..9968
                     /gene="lsrC"
                     /locus_tag="ACHKBL_001331"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrC"
                     /protein_id="HFO5818222.1"
                     /translation="MLKFIQNNREITALLAVVLLFVLPGFLDRQYLSVQTLTMVYSSA
                     QILILLAMGATLVMLTRNIDVSVGSITGMCAVLLGMLLNAGYSLPVACVATLLLGLLA
                     GFFNGVLVAWLKIPAIVATLGTLGLYRGIMLLWTGGKWIEGLPAELKQLSAPLLFGVS
                     AIGWLTIILVAFMAWLLAKTAFGRSFYVTGDNLQGARQLGVRTEAIRIVAFSLNGCMA
                     ALAGIVFASQIGFIPNQTGTVLEMKAIAACVLGGISLLGGSGAIIGAVLGAWFLTQID
                     SVLVLLRIPAWWNDFIAGMVLLAVLVFDGRLRCALERNLRRQKYARFMMPPPSVKPAS
                     SGKKREAA"
     gene            9968..10960
                     /gene="lsrD"
                     /locus_tag="ACHKBL_001332"
     CDS             9968..10960
                     /gene="lsrD"
                     /locus_tag="ACHKBL_001332"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrD"
                     /protein_id="HFO5818223.1"
                     /translation="MRIRYGWELALAALLVIEIVAFGAINPRMLDLNMLLFSTSDFIC
                     IGIVALPLTMVIVSGGIDISFGSTIGLCAIALGVLFQSGVPMPLAILLTLLLGALCGL
                     INAGLIIYTKVNPLVITLGTLYLFAGSALLLSGMAGATGYEGIGGFPMAFTDFANLDV
                     LGLPVPLIIFLICLLVFWLWLHKTHAGRNVFLIGQSPRVAVYSAIPVNRTLCALYAMT
                     GLASAVAAVLLVSYFGSARSDLGASFLMPAITAVVLGGANIYGGSGSIIGTAIAVLLV
                     GYLQQGLQMAGVPNQVSSALSGALLIVVVVGRSVSLHRQQIKEWLARRANNPLP"
     gene            10972..11994
                     /gene="lsrB"
                     /locus_tag="ACHKBL_001333"
     CDS             10972..11994
                     /gene="lsrB"
                     /locus_tag="ACHKBL_001333"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein LsrB"
                     /protein_id="HFO5818224.1"
                     /translation="MTLHRFKKIALLSALGIAVISMNVQAAERIAFIPKLVGVGFFTS
                     GGNGAQQAGKELGVDVTYDGPTEPSVSGQVQLINNFVNQGYNAIIVSAVSPDGLCPAL
                     KRAMQRGVRVLTWDSDTKPECRSYYINQGTPAQLGGMLVDMAARQVNKDKAKVAFFYS
                     SPTVTDQNQWVKEAKAKIAKEHPGWEIVTTQFGYNDATKSLQTAEGILKAYSDLDAII
                     APDANALPAAAQAAENLKNDKVAIVGFSTPNVMRPYVERGTVKEFGLWDVVQQGKISV
                     YVADALLKKGSMKTGDKLDIQGVGQVEVSPNSVQGYDYEADGNGIVLLPERVIFNKEN
                     IGKYDF"
     gene            12021..12896
                     /gene="lsrF"
                     /locus_tag="ACHKBL_001334"
     CDS             12021..12896
                     /gene="lsrF"
                     /locus_tag="ACHKBL_001334"
                     /EC_number="2.3.1.245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase"
                     /protein_id="HFO5818225.1"
                     /translation="MADLDDIKDGKDFRTDQPQQNIPFTLKGCGALDWGMQSRLSRIF
                     NPKTGKTVMLAFDHGYFQGPTTGLERIDINIAPLFEHADVLMCTRGILRSVVPPATNK
                     PVVLRASGANSILAELSNEAVALSMDDAVRLNSCAVAAQVYIGSEYEHQSIKNIIQLV
                     DAGMKAGIPTMAVTGVGKDMVRDQRYFSLATRIAAEMGAQIIKTYYVDKGFERIVAGC
                     PVPIVIAGGKKLPEREALEMCWQAIDQGASGVDMGRNIFQSDHPVAMMKAVQAVVHHN
                     ETADRAYELYLSEKQ"
     gene            12920..13210
                     /gene="lsrG"
                     /locus_tag="ACHKBL_001335"
     CDS             12920..13210
                     /gene="lsrG"
                     /locus_tag="ACHKBL_001335"
                     /EC_number="5.3.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione
                     isomerase"
                     /protein_id="HFO5818226.1"
                     /translation="MHVTLVEINVHEDKVDEFIEVFRQNHLGSVQEEGNLRFDVLQDP
                     EVNSRFYIYEAYKDEDAVAFHKTTPHYKTCVAKLESLMTGPRKKRLFNGLMP"
     gene            13267..14025
                     /gene="tam"
                     /locus_tag="ACHKBL_001336"
     CDS             13267..14025
                     /gene="tam"
                     /locus_tag="ACHKBL_001336"
                     /EC_number="2.1.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030798 - trans-aconitate
                     2-methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trans-aconitate 2-methyltransferase"
                     /protein_id="HFO5818227.1"
                     /translation="MSDWNPSLYLHFAAERSRPAVELLARVPLENIKYVADLGCGPGN
                     STTLLHQRWPAARITGIDSSPAMIAEARSTLPDCQFVEADIRNWQPEQALDLIFANAS
                     LQWLPDHYELFPHLVSLLNSHGVLAVQMPDNWLEPTHVLMREVAWEQNYPDRGREPLA
                     GVHAYYDILSEAGCEVDIWRTTYYHQMPSHQSIIDWVTATGLRPWLQDLTESEQQHFL
                     TRYHQMLEEQYPLQENGQILLAFPRLFIVARRTE"
     gene            complement(14029..14943)
                     /gene="yneE"
                     /locus_tag="ACHKBL_001337"
     CDS             complement(14029..14943)
                     /gene="yneE"
                     /locus_tag="ACHKBL_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bestrophin family protein"
                     /protein_id="HFO5818228.1"
                     /translation="MIVRPQQHWLRRIFVWHGSVLSKISSRLLLNFLFSIAVIFMLPW
                     YTHLGIKFTLAPFSILGVAIAIFLGFRNNAGYARYVEARKLWGQLMIASRSLLREVKT
                     TLPDSASVREFARLQIAFAHCLRMTLRKQPQAEVLAHYLKTEDLQRVLASNSPANRIL
                     LIMGEWLAVQRRNGQLSDILFISLNDRLNDISVVLAGCERIAYTPIPFAYTLILHRTV
                     YLFCIMLPFALVVDLHYMTPFISVLISYTFISLDCLAEELEDPFGTENNDLPLDAICN
                     AIEIDLLQMNDEAEIPAKILPDRHYQLT"
     gene            complement(15140..16591)
                     /gene="uxaB"
                     /locus_tag="ACHKBL_001338"
     CDS             complement(15140..16591)
                     /gene="uxaB"
                     /locus_tag="ACHKBL_001338"
                     /EC_number="1.1.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HFO5818229.1"
                     /translation="MKTLNRRDFPGAQYPERIIQFGEGNFLRAFVDWQIDLLNEHTDL
                     NSGVVVVRPIETSFPPSLSTQDGLYTTIIRGLNEKGEAVSDARLIRSVNREISVYSEY
                     DEFLKLAHNPEMRFVFSNTTEAGISYHAGDKFDDAPAVSYPAKLTRLLFERFSHFNGA
                     LDKGWIIIPCELIDYNGDALRELVLRYAQEWALPEAFIQWLDQANSFCSTLVDRIVTG
                     YPRDEVAKLEEELGYHDGFLDTAEHFYLFVIQGPKSLATELRLDKYPLNVLIVDDIKP
                     YKERKVAILNGAHTGLVPVAFQAGLDTVGEAMNDAEICAFVEKAIYEEIIPVLDLPRD
                     ELESFASAVTGRFRNPYIKHQLLSIALNGMTKFRTRILPQLLAGQKANGTLPARLTFA
                     LAALIAFYRGERNGETYPVQDDAHWLERYQQLWSQHRDRVIGTQELVAIVLAEKDHWE
                     QDLTQVPGLVEQVANDLDAILKKGMREAVRPLC"
     gene            complement(16819..18237)
                     /locus_tag="ACHKBL_001339"
     CDS             complement(16819..18237)
                     /locus_tag="ACHKBL_001339"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFO5818230.1"
                     /translation="MHVQPISTFRLFQEGHLLRNSIAIFVLTTLFYFIGAELRLVHEL
                     SLFWPLNGVMAGVFARYVWLNRLHYYAISYVAMLVYDAITTEWGLVSLAINFSNMMFI
                     VTVALLVARDKRLGKNKYEPVSALRLFNYCLIAALLCAIVGAIGSVSIDSLDFWPLLA
                     DWFSEQFSTGVLIVPCMLTLAIPGVLPRFKAEQMMPAIALIVSVIASVVIGGAGSLAF
                     PLPALIWCAVRYTPQVTCLLTFVTGAVEIVLVANSVIDISVGSPFSIPQMFSARLGIA
                     TMAICPIMVSFSVAAINSLMKQVALRADFDFLTQVYSRSGLYEALKSPSLKQTQHLTV
                     MLLDIDYFKSINDNYGHECGDKVLSVFAQHIQKIVGDKGLVARMGGEEFAVAVPSVNP
                     VDGLLMAEKIRKGVELQPFTWQQKTLYLTVSIGVGSGCASYRTLTDDFNKLMVEADTC
                     LYRSKKDGRNRTSTMRYGEEVV"
     gene            complement(18376..18735)
                     /gene="yneG"
                     /locus_tag="ACHKBL_001340"
     CDS             complement(18376..18735)
                     /gene="yneG"
                     /locus_tag="ACHKBL_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 domain-containing protein"
                     /protein_id="HFO5818231.1"
                     /translation="MQSLDPLFARLSRSKFRSRFRLGVKERQYCLEKGAPVIEQHAAD
                     FVAKRLAPALPANDGKQTPMRGHPVFIAQHATATCCRGCLAKWHNIPQGEALSEEQQR
                     YIVTVIYHWLVIQMNQP"
     gene            complement(18735..19661)
                     /gene="glsB"
                     /locus_tag="ACHKBL_001341"
     CDS             complement(18735..19661)
                     /gene="glsB"
                     /locus_tag="ACHKBL_001341"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase B"
                     /protein_id="HFO5818232.1"
                     /translation="MAVAMDNAILENILRQVRPLIGQGKVADYIPALATVDGSRMGIA
                     ICTVDGQLFQAGDAQERFSIQSISKVLSLVVAMRHYSEEEIWQRVGKDPSGSPFNSLV
                     QLEMEQGIPRNPFINAGALVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARS
                     EFEHSARNAAIAWLMKSFGNFHHDVTTVLQNYFHYCALKMSCIELARTFVFLANQGKA
                     IHIDEPVVTPMQARQINALMATSGMYQNAGEFAWRVGLPAKSGVGGGIVAIVPHEMAI
                     AVWSPELDDAGNSLAGIAVLEQLTKQLGRSVY"
     gene            complement(19725..21113)
                     /gene="sad"
                     /locus_tag="ACHKBL_001342"
     CDS             complement(19725..21113)
                     /gene="sad"
                     /locus_tag="ACHKBL_001342"
                     /EC_number="1.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004030 - aldehyde dehydrogenase [NAD(P)+]
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate-semialdehyde dehydrogenase"
                     /protein_id="HFO5818233.1"
                     /translation="MTITPATHAISINPATGEQLSVLPWAGADDIENALQLAAAGFRD
                     WRETNIDYRAEKLRDIGKALRVRSEEMAQMITREMGKPINQARAEVAKSANLCDWYAE
                     HGPAMLKAEPTLVENQQAVIEYRPLGTILAIMPWNFPLWQVMRGAVPIILAGNGYLLK
                     HAPNVMGCAQLIAQVFKDAGIPQGVYGWLNADNNGVSQMIKDSRIAAVTVTGSVRAGA
                     AIGAQAGAALKKCVLELGGSDPFIVLNDADLELAVKAAVAGRYQNTGQVCAAAKRFII
                     EEGIASAFTERFVAAAAALKMGDPRDEENALGPMARFDLRDELHHQVEKTLAQGARLL
                     LGGEKMAGAGNYYPPTVLANVTPEMTAFREEMFGPVAAITIAKDAEHALELANDSEFG
                     LSATIFTTDETRARQMAARLECGGVFINGYCASDARVAFGGVKKSGFGRELSHFGLHE
                     FCNIQTVWKDRI"
     gene            21214..22089
                     /gene="yneJ"
                     /locus_tag="ACHKBL_001343"
     CDS             21214..22089
                     /gene="yneJ"
                     /locus_tag="ACHKBL_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFO5818234.1"
                     /translation="MDLTQLEMFNAVAEAGSITQAATKVHRVPSNLTTRLRQLETELG
                     VDLFIRENQRLRLSPAGHNFLRYSQQILALVDEARSVVAGDEPQGLFSLGSLESTAAV
                     RIPAMLAEFNRRYPKIQFSLSTGPSGTMLEGVLEGKLNAAFIDGPINHTAIDGIPVYR
                     EELMIVTPQGHAPVTRASQVNGSNIYAFRANCSYRRHFESWFHADGAAPGTIHEMESY
                     HGMLACVVAGAGIALIPRSMLESMPGHHQVEAWPLAEQWRWLTTWLVWRRGAKTRPLE
                     AFIQLLDVSDSAKQS"
     gene            22173..22292
                     /gene="yneK"
                     /locus_tag="ACHKBL_001344"
     CDS             22173..22292
                     /gene="yneK"
                     /locus_tag="ACHKBL_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
                     /protein_id="HFO5818235.1"
                     /translation="MVTPVSISNFISLPDDFPVRNIAPQVKEEQAPFLIVIKM"
     gene            22434..23624
                     /gene="ydeA"
                     /locus_tag="ACHKBL_001345"
     CDS             22434..23624
                     /gene="ydeA"
                     /locus_tag="ACHKBL_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose MFS transporter"
                     /protein_id="HFO5818236.1"
                     /translation="MTTNTVSRKVAWLRVVTLAVAAFIFNTTEFVPVGLLSDIAQSFH
                     MQTAQVGIMLTIYAWVVALMSLPFMLMTSQVERRKLLICLFVVFIASHVLSFLSWSFT
                     VLVISRIGVAFAHAIFWSITASLAIRMAPAGKRAQALSLIATGTALAMVLGLPLGRIV
                     GQYFGWRMTFFAIGIGELITLLCLIKLLPLLPSEHSGSLKSLPLLFRRPALMSIYLLT
                     VVVVTAHYTAYSYIEPFVQNIAGFSANFATALLLLLGGAGIIGSVIFGKLGNQYASAL
                     VSTAIALLLVCLALLLPAANSEIHLGVLSIFWGIAMMIIGLGMQVKVLALAPDATDVA
                     MALFSGIFNIGIGAGALVGNQVSLHWSMSMIGYVGAVPAFAALIWSIIIFRRWPVTLE
                     EQTQ"
     gene            complement(23649..24314)
                     /gene="marC"
                     /locus_tag="ACHKBL_001346"
     CDS             complement(23649..24314)
                     /gene="marC"
                     /locus_tag="ACHKBL_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter MarC"
                     /protein_id="HFO5818237.1"
                     /translation="MLDLFKAIGLGLVVLLPLANPLTTVALFLGLAGNMNSAERNRQS
                     LMASVYVFAIMMVAYYAGQLVMDTFGISIPGLRIAGGLIVAFIGFRMLFPQQKAIDSP
                     EAKSKSEELEDEPSANIAFVPLAMPSTAGPGTIAMIISSASTVRQSSTFADWVLMVAP
                     PLIFFLVAVILWGSLRSSGAIMRLVGKGGIEAISRLMGFLLVCMGVQFIINGILEIIK
                     TYH"
     gene            24526..24960
                     /gene="marR"
                     /locus_tag="ACHKBL_001347"
     CDS             24526..24960
                     /gene="marR"
                     /locus_tag="ACHKBL_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance transcriptional
                     regulator MarR"
                     /protein_id="HFO5818238.1"
                     /translation="MKSTSDLFNEIIPLGRLIHMVNQKKDRLLNEYLSPLDITAAQFK
                     VLCSIRCAACITPVELKKVLSVDLGALTRMLDRLVCKGWVERLPNPNDKRGVLVKLTT
                     SGAAICEQCHQLVGQDLHQELTKNLTADEVATLEHLLKKVLP"
     gene            24981..25364
                     /gene="marA"
                     /locus_tag="ACHKBL_001348"
     CDS             24981..25364
                     /gene="marA"
                     /locus_tag="ACHKBL_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     MarA"
                     /protein_id="HFO5818239.1"
                     /translation="MSRRNTDAITIHSILDWIEDNLESPLSLEKVSERSGYSKWHLQR
                     MFKKETGHSLGQYIRSRKMTEIAQKLKESNEPILYLAERYGFESQQTLTRTFKNYFDV
                     PPHKYRMTNMQGESRFLHPLNHYNN"
     gene            25396..25614
                     /gene="marB"
                     /locus_tag="ACHKBL_001349"
     CDS             25396..25614
                     /gene="marB"
                     /locus_tag="ACHKBL_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance protein MarB"
                     /protein_id="HFO5818240.1"
                     /translation="MKPLLSAIATALILFSAQGVAEQTTQPVVTSCGNVVVVPTSQEQ
                     PPFDLNHMGTGSDKSDALGVPYYNQHAM"
     gene            complement(25645..26544)
                     /gene="eamA"
                     /locus_tag="ACHKBL_001350"
     CDS             complement(25645..26544)
                     /gene="eamA"
                     /locus_tag="ACHKBL_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylserine/cysteine exporter"
                     /protein_id="HFO5818241.1"
                     /translation="MSRKDGVLALLVVVVWGLNFVVIKVGLHNMPPLMLAGLRFMLVA
                     FPAIFFVARPKVPLNLLLGYGLTISFAQFAFLFCAINFGMPAGLASLVLQAQAFFTIV
                     LGAFTFGERLHGKQLAGIALAIFGVLVLIEDSLNGQHVAMLGFMLTLAAAFSWACGNI
                     FNKKIMSHSTRPAVMSLVIWSALIPIIPFFVASLILDGSATMIHSLVTIDMTTILSLM
                     YLAFVATIVGYGIWGTLLGRYETWRVAPLSLLVPVVGLASAALLLDERLTGLQFFGAV
                     LIMTGLYINVFGLRWRKAVKVGS"
     gene            26739..27926
                     /gene="ydeE"
                     /locus_tag="ACHKBL_001351"
     CDS             26739..27926
                     /gene="ydeE"
                     /locus_tag="ACHKBL_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux MFS transporter YdeE"
                     /protein_id="HFO5818242.1"
                     /translation="MNLSLRRSTSALLASSLLLTIGRGATLPFMTIYLSRQYNLSVDL
                     IGYAMTIALTIGVVFSLGFGILADKFDKKRYMLLAITAFASGFIAIPLVNNVTLVVLF
                     FALINCAYSVFATVLKAWFADNLSSTSKTKIFSINYTMLNIGWTIGPPLGTLLVMQSI
                     NLPFWLAAICSAFPMLFIQIWVKRNEKIIATETGSAWSPKVLLQDKALLWFTCSGFLA
                     SFVSGAFASCISQYVMVIADGDFAEKVVAVVLPVNAAMVVTLQYSVGRRLNPANIRAL
                     MTAGTLCFVIGLVGFIFSGNSLLLWGMSAAVFTVGEIIYAPGEYMLIDHIAPPGMKAS
                     YFSAQSLGWLGAAINPLVSGIVLTSLPPFSLFIILALVIIAAWVLMLKGIRARPWGQP
                     ALC"
     gene            28053..28148
                     /gene="mgtS"
                     /locus_tag="ACHKBL_001352"
     CDS             28053..28148
                     /gene="mgtS"
                     /locus_tag="ACHKBL_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtS"
                     /protein_id="HFO5818243.1"
                     /translation="MLGNMNVFMAVLGIILFSGFLAAYFSHKWDD"
     gene            28148..28252
                     /gene="mgtT"
                     /locus_tag="ACHKBL_001353"
     CDS             28148..28252
                     /gene="mgtT"
                     /locus_tag="ACHKBL_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtT"
                     /protein_id="HFO5818244.1"
                     /translation="MNGDNPSPNRPLVTTVYKGPDFYDGEKKPPVNRR"
     gene            complement(28367..29257)
                     /gene="dgcZ"
                     /locus_tag="ACHKBL_001354"
     CDS             complement(28367..29257)
                     /gene="dgcZ"
                     /locus_tag="ACHKBL_001354"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcZ"
                     /protein_id="HFO5818245.1"
                     /translation="MIKKTTEIDAILLNLNKAIDAHYQWLVSMFHSVVARDASKPEIT
                     DNHSYELCQFGRWIDHLGPLDNDELPYVRLMDSAHQHMHNCGRELMLAIVENHWQDAH
                     FDAFQEGLLSFTAALTDYKIYLLTIRSNMDVLTGLPGRRVLDESFDHQLRNAEPLNLY
                     LMLLDIDRFKLVNDTYGHLIGDVVLRTLATYLASWTRDYETVYRYGGEEFIIIVKAAN
                     DEEACRAGVRICQLVDNHAITHSEGHINITVTAGVSRAFPEEPLDVVIGRADRAMYEG
                     KQTGRNRCMFIDEQNVINRV"
BASE COUNT         7341 a         7484 c         7053 g         7493 t
ORIGIN      
        1 atttcattat gcatgttatt tataaaccgt gactaaacag aatattattg cgaacaaggg
       61 aagtgtcagg aaaggtcaag agcgaccaat ggtttatatg gctataaatc attagccata
      121 gttaatgttt gcctggtgta actaacacgg aattgctcta aacagataag tgtagggttt
      181 attaaagctg accgacagac tttcttaacc tggtaatatc tctcttaggt gactctccaa
      241 acatccgcga atattcccga ctgaaatggg ataagctttc gtaaccgaca gcataggctg
      301 cagtggtgac atcgtaatgt tcattcagca tcagtcgcct ggcttcattg aggcttagcc
      361 atttccgata ccggagtgga ctcattcccg caagctgtct gaaatgttga tggaaagagg
      421 gggtgttcat ttgcacgcgg tttgccagcc tgactcttca acgctgagaa gatgcggcaa
      481 acggcaaacc tgcataccag ccagcctata tccggtatgc acaggccaat tatgaaagag
      541 gagaggcttt atgttctgga accttgaagt aataacgtcg accggaaaat cactctgcat
      601 catcactcag attcaattta acataatata cattatgcgc accaacataa accaagggag
      661 aatccagccg ttggcggtca tgattgtcct gctcattaac aacggccaca ccccatatct
      721 tacttaatac cattctcggc ttaaccgacg gtgcaacctc aacacattgg tttcaactgc
      781 cgtcaccacg ttctccggaa aatctgtcgg taatgaagtc ttcacgttat ccagtgctgc
      841 cggaatcatt ctggcaaagt cactcaggat ttcatgcatc tgcacttgcg gaaatttcag
      901 cacctttgct gtcgccagaa aatgtcgcgg ataaatttta tcgattgccg tttttttgcc
      961 tttggatgcg ttaagcccca ttgccagttt gagatcgctg atgtgtattc ctgcaccacc
     1021 caataccgga aatgctgaaa tgatgtcgta aaatggcgtg agtcgataac tgccgccagc
     1081 ctgaataaat acggagaagt tttttgcatg gccatccgtt gcgccaatca accactggaa
     1141 cacctgaaac ttcataaaat catagcgatc tttcagcgcc tcgctggacc ccatcaaaaa
     1201 agccatgatc cgcgcgatgc ctgggcctcc atctgattca tatttcaccg atgaaggtaa
     1261 accgaatgtc tgacacatat cctcctgtgg caagcgaagt aaaaccgttc gctcagcatt
     1321 ccaacgcctg tcaaaacgtt cgaccgctaa cgcgcgcaca tttcccgctt taatgatttc
     1381 tgcgtccggc acattcaacc caagttcttt agccagcagc agacagtaat actcattatc
     1441 aacgctttgg ctgagatcga gcgtcgcatt gggctgcctg atttcgccaa tcggtaattt
     1501 aatgatgtgc gtcgtcggcg ttattccttt cggaatgcac cagtcattgc ctattctgag
     1561 cagcgctgtc ttctcctgcg cgccagcaac cgagatgcga aagtcatttt cttctctaat
     1621 catgcccaga gggatatctg ctttataagc cgttaatact tcttcaagcc tggcttcagt
     1681 gagctgttcc catgccatta tcgggcgcgt tatggtttca ttttcgggta ataacgttac
     1741 ggcaccaacg ctgtctcgcc ctatttctga caataaatca aacggttgtc tggatttagc
     1801 atgataacgt ttaacgatcc gatcacgtac aatcgggcta tcgggtaaca ggttatcgaa
     1861 gaagttaaat acggcatcag aggtgatatt ccccctctgc aatggcagcg aaagtgacaa
     1921 cggtctggca taacggctgg ctaaccactc cggtgcatac ttaaaggtgt gcgcgccatt
     1981 ggctaacttc gttaactcgc ctacccgctg gttgttcatc caagtgacaa gtttaggcat
     2041 taccactcca gatcttgctg ttctgttgat tctggcgagg catttttcgt gtcgcatagc
     2101 gtcattgaga gttcaagcga ctgtaaaatc ttaaaaaagg tcgtcagcgt ggtattgtca
     2161 gggttgtttt cgaaattgga aatcgtcgcc tgcttaatac caattttttt cgccagctcg
     2221 ctctgcgtcc agccattttg ctggcgaacc agtttcattg cattcgccaa ttgcgttggg
     2281 ctatagatct tctgaaagct catcatgtca tacgtccaca gcaaatttat ccgcttaagg
     2341 ggatattatc agttttatcc tttagtgagg ataagtcaat ctggaaacac atcttatccg
     2401 ctctacggga taaacactgt gatatccgcg atcgcggata aaaaggagga tttaaaagtc
     2461 gtttgaagtc ggcaatatta agccgcatgc atcttcgaca tgcggctatt cccttgggta
     2521 tcgcctttta cgttcatctt atcggtgggc gaattatcgc cgcccagcgt cgcgttgaac
     2581 gtcatcaggc cattgtttcc ggtatagtta ccgttaacgg tcagtacatt ccccggtttc
     2641 cccccttcgc tgttcatttg aaccgtgcca ccattctcca ggttaccgcc gacagtggtt
     2701 ttagcgacac gcagtgtccc ccctggcata acgttcacat cacctgcagt ggtgacattt
     2761 cccgacatca caccgctgtt atggatatta atgtgctgac ttgccatatt aatggcagag
     2821 tcggaaccga aagcaatttc accacccacg atatccgtcg tacccttata cgccgcggaa
     2881 tctttgttaa gcgtcaggat cccggtaccc agttttgtca catcaccgct accgctgata
     2941 ctgtgcgtca tcacccagct acgctcagtg gagatggtga gcaggccatt attgataacg
     3001 gaagcatcac ccaggttttt ctgctcgttg acgctaagcg cgctgcctgt atcaatgttg
     3061 aattgcccca caaagcggct attatcgcca tccagctcta cgtctgtcct ggcggttgcg
     3121 ctcacgatac ccttcccgct tatgctatta cgcagttcgc cggtcacatt ttttagcgtt
     3181 aacagaccgt cattggcaat atttcctctg cctaacccct gcgtattatc gaggctgact
     3241 tccgcatttg ggctaatgct ggttaacgca ttgtagcgtg ccttaagccc ctcgatagcc
     3301 agtgttcccc ctgtaacatt caggtttccg ccacctgtca gttcgccctg actgatccca
     3361 cctttattca ccgtcagcga cccctcttta aacaaaacag tcgaacccat ttgcccggtg
     3421 aatgtctcta ccgtctgggt cgatccatta agatcgacga tggccgcgtt gctgatgttc
     3481 agttcccggg tgttgcccag cgcgccatcc gcatcggtac gtaatgtccc catctgaacg
     3541 taggttgccc cctgatagtc gttctgaccg ttggaaagcg aaacctgtcg taccgtattg
     3601 attgccagat ccccttcgcc accgattttt gccgacatat cgaccgttgc gccataagct
     3661 ccgccatgtt cggctaacgt cagcttttgc ccaccatgga tgttcagcgc tttcagccca
     3721 tagttaacat acaaaccatc accaggtgcg gtcgtgaggc ggaatccata atcaccctcg
     3781 gcgaccaccg ttccgttttg cgcaatatgt aacgttttgt ccgcttccac ctcgtcgccc
     3841 tgtaaatcac gtaacgttaa tgaaccaccc gatccaataa ccgtttgcgc cttcaccagt
     3901 tgaacgccga catggctatc gtcgtgttcc atcaaattga gcgtcgtcag agggttattc
     3961 gccatgggat cattccacgg tttaggcacg tcgataagca cgtcgcccat cccgtttacc
     4021 tgatagttac ggcctttcca ggtgtagtca cccgcgccga caaccagcgt atcgacgctg
     4081 atatatccct ccgcaagcgt cgcagcaggt atatccgtat cgaacgtgag cgtaccgcca
     4141 ttcatggcta gtccaccaat ggattgctct cccactttta ccgatgtggt attttcactg
     4201 tcagactgca gcatcgcgta agtaagcgca gcggtgttgt cgcgttccag tgcgaaggta
     4261 ctggctttca gttgggcaac accggcgaat tcagtccctg ctgcatgggt aaagccaaac
     4321 attttgtcgg aggatgacag ctgcactcgc atcaagccat cgcctttgag cgcattgatc
     4381 agcgtgaata gcaatttccc cttgaatata gtactggtga tggaacgatg aatctgcaag
     4441 ctggcattta acaagccagc tctaaaagaa gggaaatgag aataccaata ctcaaaaact
     4501 aacagccacg gtcatcatgg tgtggctgtc aatgaaacta taacccaggc gctttccata
     4561 acgacgtcgt cagtccatga tcaaccagcc ccagctgatc ctgataaact gcctgccact
     4621 tatcgcgtga atcctgataa agttcatgct tttccgggtc tggtgtgtgc gtccgttccc
     4681 aacgaaccaa gcgttctccg gtttctgcca ttgatgaaaa aattccggca ccgacgccag
     4741 ctgcaatggc acatcctaat gcagtggctt ctttgaccac cggaatattg acgggtaatc
     4801 ccgagacatc agcgagaatt tgactccata atttcccttt tgaacctccg ccagcaaaaa
     4861 ccagcgaggt gggatgaata ttcgagaaat cagcaatttg ctgtaagtta cacgctgata
     4921 caatcgccgc attttcttcc agcgcacgga acaatgtcgc tttgttacat ttatccgggt
     4981 caatggacaa gttaataaag gaaggcgcag cgtgatacca ggttttaaag cgcattctgt
     5041 cggagaatat cggcattacg ccccacgacc caggcggcac ccgactggcc atctcttcca
     5101 gcagcgtata ggtgtcgatg cctaaacgtt cggcaatcag tttttcttcg gcacagaaag
     5161 catcgcggaa ccagcgcatg gtgagtccgg taaaaaagct tatagattca gcttgtacca
     5221 tgccaggaat aacatgggga ttaacgcgca cgttcatttc tgggtctgtc accggcgcgg
     5281 ctaagtttac aacttgctgc cagaatgtgc cgccaagaac cgcggtttgt gccggacgta
     5341 caacgcctaa ccccaggcaa ccaagctgca cgtcacctcc tccaacgacc accggagtgc
     5401 ccgccttcag accgcagagt tccgccgctt gtgaacttac cacacccagc aatgtgccgg
     5461 tttctttgac aggagaaaga atatcggcac gcaggccagc catatccagc aatgcaggtt
     5521 tccagtcacg ggtggttaga tcaataagtc ccgtggtgcc agcgttagag ggatccaccg
     5581 caagttcgcc gctgagcata taggccagcc agtcgctgat catggtgatg gttgatgcct
     5641 gacggtaaat atcggaacga tggtgcgcca gccaaagtaa tctggggatg gcgcttaaag
     5701 ccagcgtttg cccggtcgct cgataaactt tgttttcaaa ggtattgttg tgcagttctt
     5761 taagttcgtt aacttcgcgc gccgctctgg catccacatt ggcgcaggcc cagatcggtg
     5821 ttccttcatt gttatataaa acaatgcctt cacgcatcga acatgccgaa acagcagcga
     5881 tatactccgg ggctatgccg gcgttgtgca gcgcctggcg catacactca cacgccagtt
     5941 gccagttttt gttgagatca aattccatag aaccaggaac gtccggtact gccagatgcc
     6001 gccactccgc ctgtcccacg gctatttgat tgccttccgg gtcgaatatc acagcccgaa
     6061 tacttccggt gcctgcatcc agcgccatca ggtagtactt tgattctgaa ggggtaaaga
     6121 gtcgagccat tagattatcc tcggcttatg cgttctgtct gttcctctat acgttctcca
     6181 tcattcccgg taacaaggtc atgcaaattt aactacgtaa aatcgccgct gctgtgtcct
     6241 gatcggtaac cagtgcgttg atatagccgc ctttcattgc agcggcaatg gcttcggctt
     6301 tattttctcc ccctgccacg ccaacccgga cgggtatggt cttcagcgcg cttaaaggta
     6361 agccaatcag ttcgttatgt attttaatat ccgtgacaac gtcacctttt gcatcaaaaa
     6421 agtagcctaa aatgtcgcca acagcccctt ttcgaccaat cattaactgt tcgccctggc
     6481 tgatatagcc ggagcgaatg attgttgcgt cgtcttgttg actcacagcc ccaatgccga
     6541 caatcgccac atccgctgct tgcgcggcta gcagaacatc tttgacgcaa ttttcatttt
     6601 ttagcgtacg ggcaatgtca gcggaggatg cccgcaacgg agccggaata atattcacac
     6661 tgcacgccgc gttaagctgc ccgatacccg tcatataaga accgactcca ccggagagcg
     6721 tgaccaggcg aatttgctgt gacgaaataa aaccacttaa gcgttgcagc gtattcatag
     6781 ttgcctcgcc aaaaccaatc gccagcatct gttgtggttg aagtaaactc atcaacatat
     6841 gcgccgcgcc tatccctagt cgcccgccga catcagcatc cgcaagccct gggatcaccc
     6901 ggacatgttg cagcgaaaac tgacgacgta attgtgtttc atattccaga cagccttcaa
     6961 agcgagaatt aatctgtacg cgaataatgc cggattgatg ccctttctcc agtaatcgcg
     7021 acactttcaa acgggtcagg ccgagacgat cgctgatctc actctgggtc agcccgtcgt
     7081 ggtagtaaaa ccacgcaatc cgcgcgacct gttcttcttc acacattccc tgttctgaaa
     7141 ttaccgaatc gttgattgtc ataattcatt cttcactttg aacatattta aatctttaat
     7201 gcaattgttc agttcttgcg catttatatc tgtgatagca accacagttt gactctacga
     7261 gcatgaacaa acgcaaccgt gaaaatcaaa atagcataaa ttgtgatcta ttcgtcggaa
     7321 atatgtgcaa tgtccaccta aggttatgaa caaattaaaa gcagaaatac atttgttcaa
     7381 aactcacctg caaaactgaa cgggggaaat atgcaaacga gtgatacccg cgcgttaccg
     7441 ctactttgcg cccgctcggt ttataaacag tattcagggg ttaatgtcct gaaaggcatc
     7501 gattttacgt tgcatcaggg ggaggtccac gccctgctcg gcggcaatgg tgccggtaaa
     7561 tcgacgttaa tgaagattat tgccggtatt acccctgctg atagcggtac gctggagatt
     7621 gggggcaata attacaccag attaacgcca gttcatgctc atcagctggg catttatctc
     7681 gttccccagg aaccgctgct ttttccaagc ctgtcgataa aagaaaacat cctgtttggt
     7741 ctggcaaaaa aacaactctc catacagaaa atgaataact tgctggtggc gctgggctgc
     7801 cagtttgatc tgcatagtct ggcaggatcg ctggatgtcg ccgatcgcca aatggtggaa
     7861 atcctccgcg gcctgatgcg cgactcgcgg attctgatcc tcgatgaacc tacagcctcg
     7921 cttacccctg cggaaaccga acgtttgttt cgtcgcttgc aagagctgct tgctactggc
     7981 gtgggtattg tttttatctc gcataagctg ccggaaattc gccagattgc cgatcgaatt
     8041 agcgtgatgc gcgacggaac catcgcctta agcggcaaaa ccagcgaact gtctaccgac
     8101 gacattattc aggccatcac gccagcgcta cgggaaaaat cgctctctgc cagccaaaaa
     8161 ttatggctgg aattacctgg caaccgccca caacatgccg cgggaacgcc ggtgctgaca
     8221 ctggaaaatc tgaccggcga aggtttcagg aatgtcagcc tgacgctcaa tgccggagaa
     8281 attctgggct tggctgggct ggtgggagcc ggacgcacag aactggccga gacgctctat
     8341 ggtctgcgta ctttgcgtgg cggacgcatt atgctgaatg gtaaagagat caatagatta
     8401 tccaccggag atcgtttact gcgcggtctg gtttatctgc cggaagatcg ccagtcatcc
     8461 ggacttaatc tcgatgcttc actggcgtgg aacgtctgcg cccttactca taaccttcgt
     8521 ggattctggg cgaaaaccgc gaaagataat gccaccctgg aacgatatcg ccgggcgctg
     8581 aatattaaat ttaaccaacc ggaacaagct gcgcggacct tatccggcgg caaccagcaa
     8641 aaaatcctga ttgccaaatg tctggaagct tcgccgcaag ttttgattgt cgatgagccg
     8701 acgcgcggcg tggatgtctc ggcgcgtaat gatatctacc aactgttgcg cagcatcgcc
     8761 gcacaaaatg tggctgtgct gcttatctcc tctgatctgg aagagatcga actgatggca
     8821 gatcgcgtgt atgtgatgca tcagggcgaa attgcccact ctgcactgac cggacgcgat
     8881 attaatgtcg agaccattat gcgcgttgcc ttcggagata gtcagcgtca ggaggcgtca
     8941 tgctgaaatt tattcagaac aaccgtgaaa tcacggcact gctggcggtg gtgctgctgt
     9001 ttgtattacc cggttttctc gaccgtcagt atttaagtgt gcaaacgctg accatggttt
     9061 atagcagcgc gcaaatcctg atcctgctgg caatgggcgc gacgctggta atgcttacgc
     9121 gcaatattga tgtttcagtg ggttcgatta ccggaatgtg cgcggtgctg ttggggatgt
     9181 tactgaacgc ggggtattca ctacctgttg cttgtgtcgc gactttactg cttggtttgc
     9241 tcgcgggatt tttcaacggt gtcctggtcg cgtggctaaa gatccctgcc attgttgcca
     9301 cccttggcac gttagggttg tacagaggca tcatgttgct gtggactggc ggtaaatgga
     9361 ttgagggatt acccgccgaa ctgaaacaac tctccgcccc gctgctgttt ggcgtttccg
     9421 cgattggttg gttgacgata attctggtgg catttatggc ctggctgctg gcaaagacgg
     9481 cgtttggacg cagtttttat gtcacgggcg ataatttaca gggcgctcgt caactgggcg
     9541 ttcgtacgga agccattcgc attgtggcat tttcgttgaa cggctgcatg gcggcactgg
     9601 cgggaattgt gtttgcttcg cagattggtt ttatccccaa ccagaccggt accgtgctgg
     9661 agatgaaagc aattgcagcc tgcgtgctgg gcggcattag tttgctcggc ggttccggcg
     9721 cgatcatcgg tgcggtactc ggcgcatggt tcctgacgca gatcgatagt gtactggtgc
     9781 tgttgcgcat tccggcatgg tggaatgatt ttatcgcggg catggttctg ctggcggtgc
     9841 tggtgtttga tggacgcctg cgttgtgcgc tggaacgtaa tctgcggcgg caaaaatatg
     9901 cccgctttat gatgccaccg ccatccgtta aacccgcttc gtcaggtaaa aaacgggagg
     9961 ccgcgtaatg cgtattcgct acggttggga actggctctt gccgcactgc tcgttattga
    10021 gattgttgca tttggtgcaa ttaacccgcg gatgttagat ctcaatatgt tgctgttcag
    10081 caccagtgac tttatctgca ttggcattgt cgccctaccg ctgacgatgg tgattgtcag
    10141 tggcgggatc gatatttcgt ttggttcgac catcggcctc tgcgccattg cattgggcgt
    10201 actgtttcaa agtggtgtgc cgatgccgct ggcgattctt ctgaccttac tgctcggcgc
    10261 attgtgcggg ctgatcaacg ccggattaat tatctatacc aaagttaacc cgctggtgat
    10321 tacgcttggc acgctgtatc tgtttgccgg aagcgccctg ctgctttccg ggatggccgg
    10381 agcgacgggg tacgaaggta ttggtggatt cccgatggcg tttacagatt tcgctaacct
    10441 ggatgtgctg gggctccccg ttccgctgat tatcttcctg atatgtctcc tcgttttctg
    10501 gctctggctg cataaaaccc atgccggacg taacgtgttt ttaattgggc aaagcccgcg
    10561 tgtggcggtt tatagcgcga ttccagttaa ccgcaccttg tgtgcgctct atgccatgac
    10621 ggggctggcg tctgcggtcg ccgctgtgct gctggtatcg tattttggtt cagcacgttc
    10681 cgatctcggt gcgtcgtttc tgatgcccgc catcaccgcc gtagtgctcg gcggtgccaa
    10741 tatttatggt ggttctggtt ccattatcgg caccgccata gcggttttat tagtgggata
    10801 tttgcaacaa ggtttgcaaa tggcaggagt gccaaatcag gtgtccagcg ccctttccgg
    10861 tgcgctactt atcgtcgttg tcgtaggtcg ttccgttagc ctgcatcgcc agcaaattaa
    10921 agagtggctg gcgcgtcggg ccaataaccc attgccataa aggatatctt catgacactt
    10981 catcgcttta agaaaatcgc cttacttagc gcgcttggca ttgccgtaat ctctatgaat
    11041 gtgcaggcgg cagagcgtat tgcatttatt cccaaactgg ttggcgtggg attttttacc
    11101 agcggtggca acggcgcaca acaagcgggt aaagagttgg gcgttgatgt gacctacgac
    11161 gggccgacag aacccagtgt ttctggtcag gtacagttga ttaataactt cgtcaatcag
    11221 gggtataacg ccattatcgt ttctgcggtt tcgcctgatg gcttgtgtcc ggcactgaaa
    11281 cgcgccatgc aacgtggtgt gagagtgctg acctgggact ctgatactaa accggagtgc
    11341 cgctcttact acattaatca gggaacgccc gcccagttgg gaggtatgtt ggtggatatg
    11401 gcggcgcgtc aggtgaataa agacaaagcc aaagtcgcgt ttttctactc aagccccacc
    11461 gttacggacc aaaaccagtg ggtgaaagaa gcgaaagcga aaatcgccaa agagcatcca
    11521 ggctgggaaa ttgtcactac gcagtttggc tataacgatg ccactaaatc gttacaaacc
    11581 gcagaaggaa tattaaaagc ctatagcgat ctcgacgcca ttatcgcccc cgatgccaat
    11641 gccctgcccg ctgccgcaca agccgcagaa aacctgaaaa atgacaaagt tgcgattgtc
    11701 ggattcagta cgccaaacgt gatgcgtcca tatgtggaac gcggcacggt gaaagaattt
    11761 ggcctgtggg atgtggttca gcaaggcaaa atatcagttt atgtcgcgga tgcattattg
    11821 aaaaaaggat caatgaaaac gggcgacaag ctggatatcc agggcgtagg tcaggtggaa
    11881 gtctcgccaa acagcgttca gggctatgac tacgaagctg atggtaatgg catcgtactg
    11941 ttaccggagc gcgtgatatt caacaaagag aatatcggca aatacgattt ctgatgtgca
    12001 ttacttaacc ggagtacgtt atggcagatt tagatgatat taaagatggt aaagattttc
    12061 gtaccgatca accgcaacaa aatatccctt ttaccctgaa aggttgcggt gcactggatt
    12121 ggggaatgca gtcacgctta tcgcggatat ttaatccgaa aacgggtaaa accgtgatgc
    12181 tggcctttga tcatggttat tttcaggggc cgactaccgg acttgaacgc attgatataa
    12241 atatcgcccc gctgtttgaa catgccgatg tattaatgtg tacgcgcggc attttgcgca
    12301 gcgtagtccc cccggcgacc aataagccgg tggtactgcg ggcgtcaggt gcgaactcta
    12361 ttctggcgga attaagtaat gaagccgtgg cgttatcgat ggatgatgcc gtgcgcctga
    12421 acagttgcgc ggtggcggcg caggtttata tcggcagcga atatgaacat cagtcgatca
    12481 aaaatattat tcagctggtc gatgccggaa tgaaagcggg aataccgacc atggccgtga
    12541 ctggtgtggg caaagatatg gtgcgcgatc agcgttattt ctcgctcgcg actcgaatcg
    12601 ccgctgaaat gggggcacag attatcaaaa cttattatgt cgataaaggt tttgaacgga
    12661 ttgttgccgg atgtccggta cccattgtta ttgctggcgg taaaaagtta ccggagcgcg
    12721 aggcgcttga aatgtgctgg caggctatcg atcagggcgc ttctggtgtg gatatggggc
    12781 gtaatatttt ccagtctgac catccggtgg cgatgatgaa agccgtacag gcggtggtac
    12841 accataacga aacggctgat cgggcatatg aactctatct gagtgaaaaa cagtaactgc
    12901 ggatttaagg agaagaatta tgcacgtcac actggttgaa attaacgttc atgaagataa
    12961 ggttgacgag tttatcgaag tttttcgcca gaaccacctg ggctctgtac aggaagaagg
    13021 caatttgcgc ttcgatgtct tacaggaccc ggaagtgaat tcgcgctttt atatctacga
    13081 agcctataaa gatgaagacg cagtggcatt ccataaaacc acgccccact acaaaacctg
    13141 tgtcgcgaaa ctggaatctt taatgaccgg gccgcgtaaa aaacgtctgt tcaatgggtt
    13201 gatgccgtga ggcgaattta tcagttttat ctacaattgg ggtaacgcgc tgacgggagt
    13261 aaataaatgt ctgactggaa cccctcttta tatctgcact ttgccgctga acgatcgcgt
    13321 ccggcggtgg agctgcttgc cagagtgccg ctggagaata tcaaatacgt tgccgatctt
    13381 ggctgtggcc caggtaacag caccaccctg ctgcatcaac gttggcctgc ggccaggata
    13441 acaggcatcg actcgtctcc ggcgatgatt gctgaagcgc gcagtacttt gccagactgc
    13501 cagttcgtgg aagcagacat ccgcaactgg caaccggaac aggcactcga tctgattttt
    13561 gctaatgcct cactgcaatg gctgcccgac cactacgaat tgttccctca tctggtttct
    13621 ttacttaatt cgcatggtgt gttggcagta cagatgccag ataactggct agagccaacc
    13681 catgtgctca tgcgcgaagt cgcctgggaa caaaattatc cggatcgcgg gcgtgagccg
    13741 ctggctggcg ttcatgctta ctacgatatt ctgagtgaag ccggatgtga ggtcgatatc
    13801 tggcgaacca cttactatca ccagatgccg tcgcaccagt cgattatcga ttgggtgacc
    13861 gccactggat tacgtccgtg gttacaggat ctgaccgaga gcgaacagca gcattttctt
    13921 acgcgctacc atcagatgct ggaagagcaa tatcccctcc aggagaacgg acagatattg
    13981 ctggcatttc cgcgtctgtt tattgttgcc cgccgtacgg agtaattatc acgtcagctg
    14041 gtaatggcga tcgggaagaa ttttcgctgg aatttctgct tcatcgttca tctgtaacag
    14101 gtcaatttca atagcgttgc agatggcatc cagcggtaag tcattgtttt cagtaccgaa
    14161 gggatcttcc agttcttccg ccagacaatc cagcgaaata aaagtgtagg aaatcagcac
    14221 agagataaaa ggcgtcatgt aatgcaggtc cacgaccagc gcaaacggca gcatgataca
    14281 aaacagatac acggtgcgat gcaaaatcag ggtgtaggca aagggaattg gcgtataggc
    14341 aatgcgctcg catcctgcca ggaccactga aatatcatta agccgatcgt tgaggctaat
    14401 aaacaggata tctgaaagtt gtccattgcg tcgctgaacc gccaaccact cccccattat
    14461 taacaagatg cggttagctg gagagttaga agccagtaca cgctgaagat cttcagtctt
    14521 gagataatga gccagcactt ccgcctgtgg ctgtttgcgt aatgtcatgc gtaaacagtg
    14581 ggcgaaggcg atttgcagcc gggcaaactc ccttacactt gccgaatccg gcaatgtcgt
    14641 ttttacctcg cgcagtaacg accgcgaggc aatcatcaac tgcccccaaa gttttcgcgc
    14701 ttcaacgtaa cgagcgtaac cggcattatt acgaaaacca agaaaaatgg cgatggcgac
    14761 accgagaatg ctgaacggtg cgagggtgaa tttaatgccc aaatgcgtgt accagggcag
    14821 catgaaaata acagcgatag aaaagagaaa attgagtagt aagcgcgagg atatcttgga
    14881 taatactgaa ccgtgccaga caaaaatacg gcgcagccag tgttgttgtg gacgaacaat
    14941 catggttatc ttcaggcgtg gaaaagtcgc cctattaaac gtgattacga tcacattctc
    15001 aagacactac ttacaaatta cctacaagct tacaacatta acaggtgctg catgtggcac
    15061 ctgttaatga taaaggcgca taggatgttg taactaatat agtgaaataa gaaacccggt
    15121 cgaaaccggg ttcagagtat tagcacaacg gacgtacagc ttcgcgcatt ccttttttga
    15181 gaatcgcatc caggtcatta gccacctgct cgaccagacc aggcacttgc gtcaggtcct
    15241 gctcccagtg gtctttttct gccaacacaa tcgctaccag ttcctgggta ccgataacac
    15301 ggtcacgatg ctggctccag agttgctgat aacgttccag ccagtgtgca tcgtcctgta
    15361 ccggatatgt ttctccatta cgctcaccgc gataaaacgc aatcaatgcc gctaatgcga
    15421 aagtcaggcg cgccggaagt gtgccgtttg ccttctgccc tgccagcagc tgcggcagga
    15481 tacgggtgcg gaacttggtc ataccgttaa gcgcgataga cagcagctga tgcttgatgt
    15541 acggattacg gaaacgtccg gtgactgcac tggcgaaaga ttccagttca tcacgaggca
    15601 aatccagtac cggaataatc tcttcgtaga tggctttttc aacgaatgcg caaatttcag
    15661 catcgttcat cgcttcacct acggtatcca gtcctgcctg gaacgccacc ggcaccagcc
    15721 cggtgtgcgc accgttgagg attgccactt tgcgctcttt atacggttta atatcgtcaa
    15781 caatcagcac gttgagtgga tatttgtcca ggcgcagctc agtcgctaag gatttcggcc
    15841 cctgaatcac aaacaggtaa aagtgttcag cggtgtcgag gaaaccatcg tgataaccca
    15901 gttcttcttc cagtttagcc acttcatcgc gcggataacc ggtaacgata cggtccacca
    15961 gcgtagaaca gaagctgtta gcctgatcca gccattgaat aaatgcttct ggcagtgccc
    16021 actcttgcgc atagcgcaga accagttcac gcaacgcgtc gccattgtag tcgatcaact
    16081 cacacgggat gatgatccaa cctttatcca gcgcaccgtt gaaatggcta aaacgttcga
    16141 acagcagacg ggtcagtttt gccggataac ttaccgctgg cgcgtcatcg aatttatcgc
    16201 ccgcatggta gctgataccc gcttcggtgg tgttagagaa aacaaagcgc atttccgggt
    16261 tgtgcgccag tttcaggaat tcatcgtatt cactgtagac gctgatttca cgattaacag
    16321 agcgaatcag acgcgcgtcg ctgaccgctt cccccttctc attcaggcca cggataatgg
    16381 tggtgtacag accatcctgt gtgctcagtg acggtgggaa tgaagtttca atcggacgaa
    16441 caacgaccac gccagaattc agatcggtgt gctcattcag gagatcaatt tgccagtcaa
    16501 caaaggcgcg caggaagtta ccttcaccaa actgaatgat acgttctgga tactgtgcac
    16561 cgggaaaatc gcgacgattt agtgttttca caatgggttc ccttctgatt agtcatacaa
    16621 cctgttttaa ttggtacgac aggttagcaa actttaatac gccgaacccc tgttttgatc
    16681 aactcctgat gattaatgag cagttttatg agaaaagtgt ggcgcggatc atggtttaat
    16741 cgaggaacta cgccttttat tcgctcaata aagtggtaga acacattccg ttaaaatcgc
    16801 tgtgaatgaa taaagatgtc agacaacttc ctcaccgtaa cgcatagtgc tggtacggtt
    16861 gcgcccatct ttcttcgagc gatacaaaca tgtatcggct tcgaccatca atttattaaa
    16921 gtcatcggtc agcgttcggt aggatgcgca accactaccg acgccaatac ttaccgtgag
    16981 ataaagcgtt ttttgttgcc aggtgaacgg ttgcagttca acgcctttac ggattttttc
    17041 cgccattagc agaccatcga caggattcac cgatggcact gcaacagcaa attcttcgcc
    17101 gcccattcgc gccaccagcc ccttatcacc gacaatcttc tgaatatgct gggcaaacac
    17161 gcttaacact ttatcgccac attcatgtcc atagttatcg ttaatacttt tgaaatagtc
    17221 gatatcaagc agcatgacag tcagatgttg tgtctgtttc agcgatggac ttttcagcgc
    17281 ctcataaaga ccggaccgtg agtaaacctg agtcagaaaa tcaaagtcgg ctcgcagcgc
    17341 aacttgcttc attagcgaat tgatcgctgc cacgctaaaa gaaaccataa ttgggcatat
    17401 cgccatcgtg gcaataccga gacgtgcgga gaacatttgc ggaatggaga acggcgaacc
    17461 gaccgagata tcaatcaccg aatttgccac cagtacgatt tccaccgcac cggtgacaaa
    17521 ggtcaacaga catgttacct gcggcgtata gcgcactgca caccagatta atgcaggcag
    17581 cgggaacgcc agactccccg ctccgccaat gactaccgag gcaataaccg acacaataag
    17641 cgcgatagcg ggcatcatct gttctgcttt aaagcgcggc agcactccag gaattgccaa
    17701 cgtcagcata caaggcacga tcaacacgcc cgttgagaat tgctcactga accagtccgc
    17761 aagcaaaggc cagaaatcca gactatcaat actgaccgaa ccaatcgccc cgacaatagc
    17821 gcataataat gcggcaatca gacagtaatt aaatagccgt aaggcactta ctggctcata
    17881 cttatttttt cccagacgct tatcccgcgc gaccagtaag gcgacggtaa caataaacat
    17941 catattggag aaattgatag ccagtgaaac cagcccccat tcggtggtta tggcatcata
    18001 aaccagcatc gccacataac tgatcgcata gtaatgcagt cgattaagcc agacatagcg
    18061 ggcaaacacc cccgccatta cgccattcag cggccagaaa agagaaagtt cgtgaaccag
    18121 ccgtaactct gcaccaataa aatagaacag cgtggttagc acaaaaatag cgatgctatt
    18181 acgtagcaga tgaccttcct ggaacaaacg gaaagttgaa atgggctgta catgcattaa
    18241 ttatctgtcc atacaattgc gggtggcaat tatctcaacg caataagtaa attcttagaa
    18301 aggatagcat gttcgctgac aacggcggca actaaagata tcctgggaat gctattttat
    18361 cgatttccgt aattatcatg gctgattcat ctgtataacc agccagtggt agattaccgt
    18421 cacaatataa cgttgttgct cctcacttaa cgcttccccc tgaggaatgt tatgccactt
    18481 agccagacaa ccgcgacaac aggtcgcggt ggcgtgttga gcgataaaaa ccggatgccc
    18541 gcgcatgggg gtttgcttac cgtcgttagc cggtaatgcc ggagcaagac gtttagcgac
    18601 aaaatccgcc gcatgttgtt caatgactgg cgcgcctttc tccagacaat actgacgctc
    18661 tttcacaccc aaacgaaagc gagagcgaaa ttttgaacgg gataaacgcg cgaagagtgg
    18721 atcgagagac tgcattaata aactgaacgc cctaattgtt tcgtcaattg ttcaaggacg
    18781 gcaatgcccg caagcgagtt accggcatca tccagttccg gactccagac tgcgatggcc
    18841 atttcatgcg gaacaatcgc cacaataccg ccaccaacgc cagattttgc cggtagtccc
    18901 acccgccagg caaactcccc cgcgttctgg tacataccgc tggtcgccat cagcgcgtta
    18961 atttgccgcg cctgcattgg cgtcaccaca ggttcatcaa tatgaatagc tttcccctga
    19021 ttcgccagaa agacaaacgt ccgggccagc tctatacagc tcattttcag agcgcagtaa
    19081 tgaaagtagt tttgcagaac ggttgtcacg tcatgatgga aattgccaaa cgacttcatc
    19141 agccaggcga tagccgcatt tcgcgcggaa tgttcaaatt cggaacgcgc taccaccgta
    19201 tcgtaggaaa tatcagacac accacttaag ccgcgcacga cttccagcat acgttgccga
    19261 ggtgcgctta atcgcccttg caacatatcg cagaccacca gcgcaccggc attaataaac
    19321 ggattacgcg gaataccctg ctccatttcc agttgcacta aagaattgaa cggtgatcca
    19381 gacggatctt tgccgacgcg ttgccagatt tcctcttcgg agtaatgacg catggcgaca
    19441 acgagactca gcactttgga aatagactga atggaaaaac gttcttgcgc gtctccggcc
    19501 tgaaaaagct gtccgtcaac ggtacagata gcaatcccca ttcgggaacc gtctaccgta
    19561 gccagcgccg gaatataatc cgcgacttta ccctgaccaa tgagcggccg cacttgccgc
    19621 aagatgtttt ctaaaattgc attatccatg gcgactgcca ctttctactc ctggaccgca
    19681 ggtctgaaaa gacctgcgag tatatcagag ctgaatatgt agcgtcagat ccggtctttc
    19741 cacaccgtct ggatattaca gaattcgtgt aagccaaaat gggaaagctc acgcccaaag
    19801 ccactcttct tcacgccacc aaaagcgact cgcgcgtcgc tggcacagta accattgata
    19861 aacaccccac cgcattccag acgtgccgcc atctgtctgg cccgggtttc gtcggtggtg
    19921 aaaatggtcg ctgaaaggcc gaactcgcta tcattagcca gttccagtgc atgttctgca
    19981 tctttcgcaa tggtgattgc cgcaacaggg ccaaacattt cttcccgaaa cgcggtcatt
    20041 tctggggtaa cgttcgccag aaccgttggc ggatagtagt tacctgcccc agccatcttt
    20101 tccccgccca gtaacaaacg cgcaccctgc gccagggttt tttccacctg atgatgcagc
    20161 tcatcacgta aatcaaaacg agccattggt ccgagagcgt tctcttcgtc acggggatcg
    20221 cccattttca aggctgccgc agctgccaca aaacgttcgg taaatgccga agcaattccc
    20281 tcttcgataa taaagcgttt tgccgccgca caaacctgtc cggtattctg ataacgtccg
    20341 gctaccgccg ctttaaccgc cagttccaga tcggcatcgt taagcacaat aaatggatcc
    20401 gaaccgccca gttccagtac gcattttttc agtgccgctc cagcctgtgc gccaatagcc
    20461 gctcccgcac gaacacttcc ggtcactgtg acagcagcaa tgcgcgaatc tttaatcatc
    20521 tgactgacac cgttgttgtc agcattcagc cagccatata cgccttgcgg gatacccgca
    20581 tctttaaaca cctgggcaat gagctgtgca cagcccatca cattcggcgc atgtttaagt
    20641 aagtagccgt tacctgcaag aatgatggga accgcgccac gcatcacctg ccataacgga
    20701 aaattccacg gcataatcgc cagaattgtc cccaacggtc gatactcaat aactgcctgc
    20761 tgattttcca ccagcgtagg ttccgccttc agcattgccg gaccatgttc tgcataccag
    20821 tcacacaaat tcgccgattt cgccacttca gcgcgcgcct ggttgattgg tttgcccatt
    20881 tcacgggtga tcatttgcgc catttcttcg ctacggacac gcagagcctt accgatatca
    20941 cgcagttttt cagcacgata atctatattt gtctcgcgcc agtcgcgaaa gcctgctgcc
    21001 gccagctgaa gtgcgttttc gatatcatca gcgccagccc acggcagcac agaaagttgt
    21061 tcaccggtgg caggatttat cgaaattgca tgagttgccg gagtaatggt catcggggta
    21121 tctcctttat gagttatggt aaaaagatac gcagatttac tcttgcttta aaatgaataa
    21181 tattaagcca cttattcacg aatcgagaat attatggatc tgacccaact ggagatgttc
    21241 aacgcggttg ccgaagctgg cagcattacg caggctgcaa caaaagtgca tcgcgtgccg
    21301 tcgaatctca ctacccgtct acgccagctg gaaacagaac tgggggttga tctgtttatt
    21361 cgcgagaatc agcgtttacg tctctcgcct gccgggcata actttttacg ctatagccaa
    21421 caaattctcg cgttagtgga tgaagcgcgg agcgttgtcg ctggcgatga gccgcaaggt
    21481 ttattttctc ttggttcgct ggaaagcacc gcagcagtgc gcattccggc catgctggcg
    21541 gaatttaacc gtcgttatcc caaaattcag ttttcacttt ccaccggccc ttccggcact
    21601 atgctggaag gggtgctgga gggaaaactg aatgcggcgt ttattgatgg gcccattaac
    21661 catactgcca ttgacgggat accggtgtac cgcgaggagc tgatgattgt cacgccacaa
    21721 ggacatgcgc cagtaacccg tgccagtcag gttaatggca gtaacattta tgccttccgc
    21781 gccaattgtt cgtatcgtcg ccacttcgag agctggtttc atgctgacgg tgccgctccg
    21841 ggaactattc acgagatgga gtcgtatcac gggatgttgg cctgtgtggt cgcaggagca
    21901 ggcattgcgc ttattccacg ctcaatgctg gaaagtatgc cggggcatca ccaggttgaa
    21961 gcgtggccgt tagctgagca atggcgttgg ttaacaacct ggctggtctg gcgtcgcggt
    22021 gcgaaaacgc gtccgcttga ggcatttatt caactactgg atgtgtctga ctcggcaaaa
    22081 cagagttagt aatgagctat ttttgatagt tcttgcggct aatatgctct atatagtgat
    22141 gctccgatga cttatgacta tatggggcaa atatggttac gccagtaagc atcagcaatt
    22201 tcatctcact tcctgatgat tttcccgttc gtaatattgc acctcaggta aaagaagaac
    22261 aggccccctt cctgatcgtc attaaaatgt gatgttaaca actctctgat gcgtgacaga
    22321 acaaccgtca gagagatgga aatgcgatcc agatcacaaa tgcattgtat tcacatcatt
    22381 aaccgtttta agatcatttc atcacttttt cgcaactcac ccgataatct gttatgacaa
    22441 caaacactgt ttcccgcaaa gtggcgtggc tacgggtcgt tacgctggca gtcgccgcct
    22501 tcatcttcaa caccaccgaa tttgtcccag ttggcctgct ctctgacatt gcgcagagtt
    22561 ttcacatgca aaccgctcag gtcggcatta tgttgaccat ttacgcatgg gtagtagcgc
    22621 taatgtcatt gccttttatg ttaatgacca gccaggttga acggcgcaaa ttactgatct
    22681 gcctgtttgt ggtgtttatt gccagccacg tactgtcgtt tttatcgtgg agttttaccg
    22741 ttctggtgat cagtcgcatt ggtgtggctt ttgcacatgc gattttctgg tcgattacgg
    22801 cgtctctggc gatccgtatg gctccggccg ggaagcgagc acaggcattg agtttaattg
    22861 ccaccggtac tgcactggcg atggtcttag gtttacctct cgggcgcatt gtggggcagt
    22921 atttcggttg gcgaatgacc ttcttcgcga ttggtatcgg ggagcttatc acccttttgt
    22981 gcctgattaa gttacttccc ttactgccca gtgagcattc cggttcgctg aaaagcctcc
    23041 cgctattatt ccgccgccca gcattgatga gcatttattt gttaactgtg gtggttgtca
    23101 ccgcccatta cacggcatac agctatatcg agccttttgt gcaaaacatt gcgggattca
    23161 gcgccaactt tgccacggca ttactgttat tactcggtgg tgcgggcatt attggcagcg
    23221 tgattttcgg taaactgggt aatcagtatg cgtctgcgtt ggtaagtacg gcgattgcgc
    23281 tgttgctggt gtgcctggca ctgctgctac ctgcggcgaa cagtgaaata cacctcgggg
    23341 tgctgagtat tttctggggg atagcgatga tgatcatcgg gcttggtatg caggttaaag
    23401 tgctggcgct ggcaccagat gctaccgacg tcgcgatggc gctattctcc gggatattta
    23461 atattggaat cggcgcgggt gcgttggtag gtaatcaggt aagtctgcac tggtcaatgt
    23521 cgatgattgg ttatgtgggc gcggtgcctg cctttgccgc gttaatatgg tcaatcatca
    23581 ttttccgccg ctggccagtg acactcgaag aacagacgca atagttgaaa ggcccatttg
    23641 ggcctttttt agtggtacgt tttaatgatt tccaggatgc cgttaataat aaactgcacc
    23701 cccatacata ccagcaggaa tcccatcaga cgggagatcg cttcaatgcc ccccttaccc
    23761 accagccgca taattgcgcc agagctgcgt agacttcccc acaaaataac cgccaccagg
    23821 aaaaagatca gcggcggcgc aaccatcagt acccaatcag caaaggttga actctgacgc
    23881 actgtggacg ccgagctaat aatcatcgct atggttcccg gaccggcggt acttggcatt
    23941 gccagtggta caaaagcaat attggcgctg ggttcatctt ccagctcttc cgacttgctt
    24001 ttcgcttctg gtgaatcaat cgctttctgt tgcggaaaga gcatccgaaa accgataaac
    24061 gcgacgatta agccgcctgc aattcgcaga ccgggaatcg aaatgccaaa tgtatccatc
    24121 accagttgcc cggcgtaata cgccaccatc atgatggcaa agacgtacac cgaggccatc
    24181 aacgactgac gattacgttc ggcactgttc atgttgcctg ccaggccaag aaataacgcg
    24241 acagttgtta atgggttagc taacggtagt aacaccacca gccccaggcc aattgcttta
    24301 aataaatcta acattggtgg ttgttatcct gtgtatctgg tttatcagcg aaaagtataa
    24361 ggggtaaaca acgataaagt gtcactcttt agctaacctt gcatcgcatt gaccaaaact
    24421 tgaaccgatt tagcaaaacg tggcatcggt caattcattc atttgactta tacttgcctg
    24481 ggcaatatta tcccttgcaa ctaattactt gccagggcaa ctaatgtgaa aagtaccagc
    24541 gatctgttca atgaaattat tccattgggt cgcttaatcc atatggttaa tcagaagaaa
    24601 gatcgcctgc ttaacgagta tctgtctccg ctggatatta ccgcggcaca gtttaaggtg
    24661 ctctgctcta tccgctgcgc ggcgtgtatt actccggttg aactgaaaaa ggtattgtcg
    24721 gtcgacctgg gggcgctgac ccgtatgctg gatcgcctgg tctgtaaagg ctgggtggaa
    24781 aggttgccga acccgaatga caagcgcggc gtactggtaa aacttaccac cagcggcgcg
    24841 gcaatatgtg aacaatgcca tcaattagtt ggccaggacc tgcatcaaga attaacaaaa
    24901 aacctgacgg cggacgaagt ggcaacactt gagcatttgc ttaagaaagt cctgccgtaa
    24961 acaaaaaaag aggtatgacg atgtccagac gcaatactga cgctattacc attcatagca
    25021 ttttggactg gatcgaggac aacctggaat cgccactgtc actggagaaa gtgtcagagc
    25081 gttcgggtta ctccaaatgg cacctgcaac ggatgtttaa aaaagaaacc ggtcattcat
    25141 taggccaata catccgcagc cgtaagatga cggaaatcgc gcaaaagctg aaggaaagta
    25201 acgagccgat actctatctg gcagaacgat atggcttcga gtcgcaacaa actctgaccc
    25261 gaaccttcaa aaattacttt gatgttccgc cacataaata ccggatgacc aatatgcagg
    25321 gtgaatcgcg ctttttacat ccattaaatc attacaacaa ctagttgata acgtgacaac
    25381 gtcactgagg caatcatgaa accactttta tccgcaatag caactgcgct tattctcttt
    25441 tctgcgcagg gcgttgcgga acaaaccacg cagccggttg ttacttcctg tggcaatgtc
    25501 gtggttgttc ccacatcgca ggaacaacca ccgtttgatt taaatcacat gggtactggc
    25561 agtgataagt cggatgcgct cggcgtgccc tattataatc aacacgctat gtagtttgtt
    25621 ctggccccga catatcgggg cttattaact tcccactttt accgctttac gccaccgcaa
    25681 gccaaataca ttgatataca gcccggtcat aatgagcacc gcaccgaaaa attgtagacc
    25741 cgttaagcgt tcatccaata atagtgccgc acttgccagt cctactacgg gcaccagtaa
    25801 cgataacggt gcaacccgcc aggtttcata gcgtcccagt aacgtccccc agatcccata
    25861 accaacaatt gtcgccacaa acgccagata catcagagac aagatggtgg tcatatcgat
    25921 agtaaccaga ctgtgaatca tggttgcgga accatcgaga atcagcgagg caacaaagaa
    25981 gggaatgatt gggattaatg cgctccagat taccagcgac atcaccgccg ggcgcgttga
    26041 gtgcgacatg atctttttat tgaagatgtt gccacacgcc caactaaatg ctgccgccag
    26101 ggtcaacata aagccgagca tcgccacatg ctgaccgttc agactatctt cgattaacac
    26161 cagtacgcca aaaatcgcta aggcgatccc cgccaattgt ttgccatgca gtcgctcccc
    26221 gaaagtaaac gcgccaagca cgatagtaaa aaacgcctgt gcctgtaaca ccagcgaagc
    26281 cagtccagca ggcataccaa agttaatggc gcaaaacaga aaggcaaact gcgcaaaact
    26341 gatggttaat ccatacccca gcagcaaatt cagtggtact ttcggtcgag cgacaaaaaa
    26401 gatggccgga aaagcgacca gcataaagcg caaaccggcc agcatcagcg gtggcatgtt
    26461 atgaagcccc actttgatga ccacaaaatt cagcccccat acgaccacta ccagtagcgc
    26521 caacacccca tcttttcgcg acattatacc gcctctgaat ttcatctttt gtaagcaatc
    26581 aacttagctg aatttacttt tctttaacag tcgattcgtt agtcaccgtt tacgacggca
    26641 ttaatgcgca aataagtcgc tatacttcgg atttttgcca tgctatttct ttacatctct
    26701 aaaacaaaac ataacgaaac gcactgccgg acagacaaat gaacttatcc ctacgacgct
    26761 ctaccagcgc ccttcttgcc tcgtcgttgt tattaaccat tggacgcggc gctacgctgc
    26821 cgtttatgac catttacttg agtcgccagt acaacctgag tgtcgatcta atcggttatg
    26881 cgatgacaat tgcgctcact attggcgtcg tttttagcct cggttttggt atcctggcgg
    26941 ataagttcga caagaaacgc tatatgttac tggcaattac cgccttcgcc agcggtttta
    27001 ttgccattcc tttagtgaat aacgtgacgc tggtggtgct cttttttgcc ctcattaact
    27061 gcgcctattc cgtttttgct accgtgttga aagcctggtt tgccgacaat ctttcgtcca
    27121 ccagcaaaac gaaaatcttc tcaatcaact acaccatgct caacattggc tggaccatcg
    27181 gtccgccgct cggcacgttg ttggtgatgc agagcatcaa tctgcccttt tggctggcag
    27241 ctatctgttc cgcgtttccc atgcttttca ttcaaatttg ggtaaagcgc aacgagaaaa
    27301 tcatcgccac ggaaacaggc agtgcctggt cgccgaaagt tttattacaa gataaagcac
    27361 tgttgtggtt tacctgctct ggttttctgg cttcctttgt tagcggcgca tttgcctcgt
    27421 gcatttcaca atatgtgatg gtgattgctg atggtgattt tgccgaaaag gtggttgcgg
    27481 ttgttcttcc ggttaatgct gccatggtag ttacgttgca atattccgtg ggccgccgac
    27541 ttaacccggc taacatccgc gcgctgatga cagcaggcac cctctgtttc gtcattgggc
    27601 tggtcggttt tattttttcc ggcaacagcc tgctattgtg gggtatgtca gctgcggtat
    27661 ttactgtcgg tgaaatcatt tatgcgccgg gcgagtatat gttgattgac catattgcgc
    27721 cgccaggaat gaaagccagc tatttttccg ctcagtcttt aggctggctt ggtgcggcga
    27781 ttaacccatt agtgagtggc atagtgctta ccagtctgcc gcctttctcg ctgtttatca
    27841 tcttagcgtt ggtgatcatt gctgcgtggg tgctgatgtt aaaaggaatt cgtgcaagac
    27901 cgtgggggca gcccgcgctt tgttgattta agtcgaacac aataaaaatt taattcagcc
    27961 ttcgtttagg ttacctctgc taatatcttt ctcattgaga tgaaaattaa ggtaagcgag
    28021 gaaacacacc acaccataaa cggaggcaaa taatgctggg taatatgaat gtttttatgg
    28081 ccgtactggg aataatttta ttttctggtt ttctggccgc gtatttcagc cacaaatggg
    28141 atgactaatg aacggagata atccctcacc taaccggccc cttgttacaa ctgtgtacaa
    28201 ggggcctgat ttttatgacg gcgaaaaaaa accgccagta aaccggcggt gaatgcttgc
    28261 atggatagat ttgtgttttg cttttacgct aacaggcatt ttcctgcact gataacggat
    28321 cgttgacaca gtagcatcag ttttctcaat gaatgttaaa cggagcttaa actcggttaa
    28381 tcacgttttg ttcgtcaata aacatacagc gatttcttcc ggtttgctta ccctcataca
    28441 ttgcccggtc cgctcttcca atgaccacat ccagaggctc ttcaggaaat gcgcgactca
    28501 cacctgctgt cacggtaatg ttgatatgcc cttcagaatg tgtgatggca tggttatcga
    28561 ctaactggca aattctgaca cctgcacgac atgcttcttc atcattagcc gctttgacaa
    28621 taatgataaa ttcttcgcca ccgtagcgat aaaccgtttc gtaatcgcgc gtccaactgg
    28681 ctaagtaagt tgccagggtg cgtaatacta catcgccgat taaatgcccg taggtatcat
    28741 taaccaattt aaatcggtca atatccaaca acattaaata aagattcaga ggctcagcgt
    28801 tgcgtaactg atgatcaaag gattcatcaa gaacccgacg acccggcaat cccgtcaaaa
    28861 catccatatt gctacggatc gtcagcaaat aaattttgta atcggttaat gccgcagtaa
    28921 aagaaagcaa cccttcctga aaggcgtcga aatgcgcgtc ctgccagtga ttttcaacaa
    28981 tagccagcat taattcccga ccacagttat gcatatgttg atgggcagaa tccattagcc
    29041 gaacgtaagg taattcatcg ttatcgagtg gtcccagatg atcaatccac cgaccaaact
    29101 ggcacagttc ataagaatgg ttatccgtta tttctggctt actggcatct ctcgcgacca
    29161 cgctgtgaaa catacttacc agccactggt agtgggcatc gatagcctta ttgagattta
    29221 acaagatggc atcaatttcc gttgtcttct tgatcattgc cactcctttt tcacagttcc
    29281 ttgtgcgcgc tattctaacg agagaaaagc aaaattacgt caatattttc atagaaatcc
    29341 gaagttatga gtcatctctg agataacatt g
//
LOCUS       DAZFLD010000036        29119 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.036, whole genome shotgun sequence.
ACCESSION   DAZFLD010000036 DAZFLD010000000
VERSION     DAZFLD010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29119)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29119)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29119
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.036"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            160..1542
                     /gene="ydgI"
                     /locus_tag="ACHKBL_001355"
     CDS             160..1542
                     /gene="ydgI"
                     /locus_tag="ACHKBL_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFO5818246.1"
                     /translation="MEKKLGLSALTALVLSSMLGAGVFSLPQNMAAVASPAALLIGWS
                     ITGAGILLLTFAMLILTRIRPELDGGIFTYAREGFGELIGFCSAWGYWLCAVIANVSY
                     LVIVFSALSFFTDTPELRLFGDGNTWQSIVGASALLWIVHFLILRGVQTAASINLVAT
                     LAKLLPLGLFVVLAMMMFKLDTFKLDFTGLALGVPVWEQVKNTMLITLWVFIGVEGAV
                     VVSARARNKRDVGKATLLAVLSALGVYLLVTLLSLGVVARPELAEIRNPSMAGLMVEM
                     MGPWGEIIIAAGLIVSVCGAYLSWTIMAAEVPFLAATHKAFPRIFARQNAQAAPSASL
                     WLTNICVQICLVLIWLTGSDYNTLLTIASEMILVPYFLVGAFLLKIATRPLHKAVGVG
                     ACIYGLWLLYASGPMHLLLSVVLYAPGLLVFLYARKTHTHDNVLNRQEMVLIGMLLIA
                     SVPATWMLVG"
     gene            1579..2301
                     /gene="folM"
                     /locus_tag="ACHKBL_001356"
     CDS             1579..2301
                     /gene="folM"
                     /locus_tag="ACHKBL_001356"
                     /EC_number="1.5.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydromonapterin reductase"
                     /protein_id="HFO5818247.1"
                     /translation="MGKAQPLPILITGGGRRIGLALAWHFINQKQPVIISYRTHYPAI
                     DGLINAGAQCIQADFSTNDGVMAFADEVLKSTHGLRAILHNASAWMAEKPGTPLTDVL
                     ACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHIAYAASKAALD
                     NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVD
                     YLLTSCFVTGRSFPLDGGRHLR"
     gene            complement(2298..2633)
                     /gene="ydgC"
                     /locus_tag="ACHKBL_001357"
     CDS             complement(2298..2633)
                     /gene="ydgC"
                     /locus_tag="ACHKBL_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlpM family protein"
                     /protein_id="HFO5818248.1"
                     /translation="MGLVIKAALGALVVLLIGVLAKTKNYYIAGLIPLFPTFALIAHY
                     IVASERGIEALRATIIFSMWSIIPYFVYLASLWYFTGMMRLPAAFVGSVACWGISAWV
                     LIICWIKLH"
     gene            2762..3481
                     /gene="rstA"
                     /locus_tag="ACHKBL_001358"
     CDS             2762..3481
                     /gene="rstA"
                     /locus_tag="ACHKBL_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RstA"
                     /protein_id="HFO5818249.1"
                     /translation="MNTIVFVEDDAEVGSLIAAYLAKHDMQVTVEPRGDRAEETILQE
                     NPDLVLLDIMLPGKDGMTICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKT
                     TPPAVLLARLRLHLRQNEQATLSKGLQETSLTPYKALHFGTLTIDPINRVVTLANTEI
                     SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAA
                     EPYRIKTVRNKGYLFAPHAWE"
     gene            3485..4786
                     /gene="rstB"
                     /locus_tag="ACHKBL_001359"
     CDS             3485..4786
                     /gene="rstB"
                     /locus_tag="ACHKBL_001359"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RstB"
                     /protein_id="HFO5818250.1"
                     /translation="MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMN
                     SSLYLMRSELREIPPHDWGKTLKEMDLNLSFDLRVEPLSKYHLDDISMHRLRGGEIVA
                     LDDQYTFLQRIPRSHYVLAVGPVPYLYYLHQMRLLDIALIAFIAISLAFPVFIWMRPH
                     WQDMLKLEAAAQRFGDGHLNERIHFDEGSSFERLGVAFNQMADNINALIASKKQLIDG
                     IAHELRTPLVRLRYRLEMSDNLSAVESQALNRDISQLEALIEELLTYARLDRPQNELH
                     LSEPDLPLWLSTHLADIQAVTPDKTVRIKTLAQGHYAALDMRLMERVLDNLLNNALRY
                     CHSTVETSLLLSGNRATLIVEDDGPGIAPENREHIFEPFVRLDPSRDRSTGGCGLGLA
                     IVHSIALAMGGTVNCDTSELGGARFSFSWPLWHNIPQFTSA"
     gene            4862..5791
                     /gene="tus"
                     /locus_tag="ACHKBL_001360"
     CDS             4862..5791
                     /gene="tus"
                     /locus_tag="ACHKBL_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006274 - DNA replication termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HFO5818251.1"
                     /translation="MARYDLVDRLNTTFRQMEQELAAFAAELEQHKLLVARVFSLPEV
                     KKEDEHNPLNRIEVKQHLGNDAQSLALRHFRHLFIQQQSENRSSKTAVRLPGVLCYQV
                     DNLSQAALVSHIQHINQLKTTFEHIVTVESELPTAARFEWVHRHLPGLITLNAYRTLT
                     VLHDPATLRFGWANKHIIKNLHRDEVLAQLEKSLKSPRSVAPWTREEWQRKLEREYQD
                     IATLPQDAKLKIKRPVKVQPIARVWYKGDQKQVQHACPTPLIALINRDNGAGVPDVGE
                     LLNYDADNVQHRYKPQAQPLRLIIPRLHLYVAD"
     gene            complement(5788..7191)
                     /gene="fumC"
                     /locus_tag="ACHKBL_001361"
     CDS             complement(5788..7191)
                     /gene="fumC"
                     /locus_tag="ACHKBL_001361"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001099101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045239 - tricarboxylic acid cycle
                     heteromeric enzyme complex [Evidence IEA];
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HFO5818252.1"
                     /translation="MNTVRSEKDSMGAIDVPADKLWGAQTQRSLEHFRISTEKMPTSL
                     IHALALTKRAAAKVNEDLGLLSEEKASAIRQAADEVLAGQHDDEFPLAIWQTGSGTQS
                     NMNMNEVLANRASELLGGVRGMERKVHPNDDVNKSQSSNDVFPTAMHVAALLALRKQL
                     IPQLKTLTQTLSEKSRVFADIVKIGRTHLQDATPLTLGQEISGWVAMLEHNLKHIEYS
                     LPHVAELALGGTAVGTGLNTHPEYARRVADELAVITCAPFVTAPNKFEALATCDALVQ
                     AHGALKGLAASLMKIANDVRWLASGPRCGIGEISIPENEPGSSIMPGKVNPTQCEALT
                     MLCCQVMGNDVAINMGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIE
                     PNRERINQLLNESLMLVTALNTHIGYDKAAEIAKKAHKEGLTLKAAALALGYLSEAEF
                     DSWVRPEQMVGSMKAGG"
     gene            complement(7334..8980)
                     /gene="fumB"
                     /locus_tag="ACHKBL_001362"
     CDS             complement(7334..8980)
                     /gene="fumB"
                     /locus_tag="ACHKBL_001362"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HFO5818253.1"
                     /translation="MSNKPFHYQAPFPLKKDDTEYYLLTSEHVSVSEFEGQEILKVAP
                     EALTLLARQAFHDASFMLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKEL
                     LIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIW
                     IEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVG
                     RDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQ
                     LQAQGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK"
     gene            9179..10354
                     /gene="manA"
                     /locus_tag="ACHKBL_001363"
     CDS             9179..10354
                     /gene="manA"
                     /locus_tag="ACHKBL_001363"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416130.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009298 - GDP-mannose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HFO5818254.1"
                     /translation="MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKS
                     SSRVQNAAGDIVSLRDVIESNKSTLLGEAVAKRFGELPFLFKVLCAAQPLSIQVHPNK
                     RNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
                     VAGAHPAIAHFLQQPDAERLSELFASLLNMQGEEKSRALAILKSALDSQQGEPWQTIR
                     LISECYPEDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAG
                     LTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELDFPIPVDDFAFSLHDLSDKETT
                     ISQQSAAILFCVEGDATLCKGSQQLQLKPGESAFIAANESPVTVRGHGRLARVYNKL"
     gene            10455..11963
                     /gene="ydgA"
                     /locus_tag="ACHKBL_001364"
     CDS             10455..11963
                     /gene="ydgA"
                     /locus_tag="ACHKBL_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HFO5818255.1"
                     /translation="MNKSLVAVGVIVALGVVWTGGAWYTGKKIETHLEDMVAQANAQL
                     KLTAPESNLEVSYQNYHRGVFSSQLQLLVKPIAGKVNPWIKSGQSVIFNESVDHGPFP
                     LAQLKKLNLIPSMASIQTTLVNNEVSKPLFDMAKGETPFEINSRIGYSGDSSSDISLK
                     PLNYEQKDEKVAFSGGEFQLNADRDGKAISLSGEAQSGRIDAVNEYNQKVQLTFNNLK
                     TDGSSTLASFGERVGNQKLSLEKMTISVEGKELALLEGMEISGKSDLVNDGKTINSQL
                     DYSLNSLKVQNQDLGSGKLTLKVGQIDGEAWHQFSQQYNAQTQALLAQPEIANNPELY
                     QEKVTEAFFSALPLMLKGDPVITIAPLSWKNSQGESALNLSLFLKDPATTKEAPQTLA
                     QEVDRSVKSLDAKLTIPVDMATELMTQVAKLEGYQEDQAKKLAKQQVEGASAMGQMFR
                     LTTLQDNTITTSLQYANGQITLNGQKMPLEDFVGMFAMPALNVPAVPAIPQQ"
     gene            complement(12008..13273)
                     /gene="uidC"
                     /locus_tag="ACHKBL_001365"
     CDS             complement(12008..13273)
                     /gene="uidC"
                     /locus_tag="ACHKBL_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide uptake porin UidC"
                     /protein_id="HFO5818256.1"
                     /translation="MRKIVAMAVICLTAASGLTSAYAAQPADDEAGLRIRLKNELRRA
                     DKPSAGTGRDIYAWVQGGLLDFNSGYYSNIIGVEGGAYYVYKLGARADMSTRWYLDGD
                     KSFGFALGAVKIKPSENSLLKLGRFGTDYSYGSLPYRIPLMAGSSQRTLPTVSEGALG
                     YWALTPNIDLWGMWRSRVFLWTDSTTGIRDEGVYNSQTGKYDKHRARSFLAASWHDDT
                     SRYSLGASVQKDVSNQIQSILEKSIPLDPNYTLKGELLGFYAQLEGLSRNTSQPNETA
                     LVSGQLTWNAPWGSVFGSGGYLRHAMNGAVVDTDIGYPFSLSLDRNREGMQSWQLGVN
                     YRLTPQFTLTFAPIVTRGYESSKRDVRIEGAGILGGMNYRVSEGPLQGMNFFLAADKG
                     REKRDGSALGDRLNYWDVKMSIQYDFMLK"
     gene            complement(13312..14685)
                     /gene="uidB"
                     /locus_tag="ACHKBL_001366"
     CDS             complement(13312..14685)
                     /gene="uidB"
                     /locus_tag="ACHKBL_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
                     /protein_id="HFO5818257.1"
                     /translation="MNQQLSWRAIVGYSLGDVANNFAFAMGALFLLSYYTDVAGVGAA
                     AAGTMLLLVRVFDAFADVFAGRVVDSVNTRWGKFRPFLLFGTAPLMIFSVLVFWVPTD
                     WSHSSKVVYAYLTYMGLGLCYSLVNIPYGSLATAMTQQPQSRARLGAARGIAASLTFV
                     CLAFLIGPSIKNSSPEEMVSVYHFWTIVLAIAGMVLYFICFKSTRENVVRIVAQPSLK
                     ISLQTLKRNRPLFMLCIGALCVLISTFAVSASSLFYVRYVLNDTGLFTVLVLVQNLVG
                     TVASAPLVPGMVARIGKKNTFLIGALLGTCGYLLFFWVSVWSLPVALVALAIASIGQG
                     VTMTVMWALEADTVEYGEYLTGVRIEGLTYSLFSFTRKCGQAIGGSIPAFILGLSGYI
                     ANQAQTPEVIMGIRTSIALVPCGFMLLAFVIIWFYPLTDKKFKEIVVEIDNRKKVQQQ
                     LINDITS"
     gene            complement(14682..16493)
                     /gene="uidA"
                     /locus_tag="ACHKBL_001367"
     CDS             complement(14682..16493)
                     /gene="uidA"
                     /locus_tag="ACHKBL_001367"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HFO5818258.1"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESVLQEIRA
                     IAVPGSFNDQFADADIRNYVGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQ
                     EVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY
                     FHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRD
                     ADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAV
                     KDEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYA
                     EEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAI
                     KELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVD
                     TLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGN
                     KKGIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     gene            complement(16880..17470)
                     /gene="uidR"
                     /locus_tag="ACHKBL_001368"
     CDS             complement(16880..17470)
                     /gene="uidR"
                     /locus_tag="ACHKBL_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator UidR"
                     /protein_id="HFO5818259.1"
                     /translation="MMDNMQTEAQPTRTRILNAAREIFSENGFHSASMKAICKSCAIS
                     PGTLYHHFISKEALIQAIILQDQERALARFREPIEGIHFVDYMVESIVSLTHEAFGQR
                     ALVVEIMAEGMRNPQVAAMLKNKHMTITEFVAQRMRDAQQKGEISPDINTAMTSRLLL
                     DLTYGVLADIEAEDLSREASFAQGLRAMIGGILTAS"
     gene            complement(17691..18458)
                     /gene="hdhA"
                     /locus_tag="ACHKBL_001369"
     CDS             complement(17691..18458)
                     /gene="hdhA"
                     /locus_tag="ACHKBL_001369"
                     /EC_number="1.1.1.159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-alpha-hydroxysteroid dehydrogenase"
                     /protein_id="HFO5818260.1"
                     /translation="MFNSDNLRLDGKCAIITGAGAGIGKEIAITFATAGASVVVSDIN
                     ADAANHVVDEIQQLGGQAFACRCDITSEQELSALADFAVSKLGKVDILVNNAGGGGPK
                     PFDMPMADFRRAYELNVFSFFHLSQLVAPEMEKNGGGVILTITSMAAENKNINMTSYA
                     SSKAAASHLVRNMAFDLGEKNIRVNGIAPGAILTDALKSVITPEIEQKMLQHTPIRRL
                     GQPQDIANAALFLCSPAASWVSGQILTVSGGGVQELN"
     gene            complement(18570..19598)
                     /gene="malI"
                     /locus_tag="ACHKBL_001370"
     CDS             complement(18570..19598)
                     /gene="malI"
                     /locus_tag="ACHKBL_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mal regulon transcriptional regulator MalI"
                     /protein_id="HFO5818261.1"
                     /translation="MATAKKITIHDVALAAGVSVSTVSLVLSGKGRISTATGERVNAA
                     IEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLLHG
                     GKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRMAEEKAIPVIFASRASYLDDVD
                     TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSD
                     WVLECSSSQKQAAEAITALLRHNPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDR
                     YFEQQVSLAAFTDATPTTLDDIPVTWASTPARELGTTLADRMMQKITHEETHSRNLII
                     PARLIAAK"
     gene            19773..21365
                     /gene="malX"
                     /locus_tag="ACHKBL_001371"
     CDS             19773..21365
                     /gene="malX"
                     /locus_tag="ACHKBL_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HFO5818262.1"
                     /translation="MTAKTAPKVTLWEFFQQLGKTFMLPVALLSFCGIMLGIGSSLSS
                     HDVITLIPVLGNPVLQAIFTWMSKIGSFAFSFLPVMFCIAIPLGLARENKGVAAFAGF
                     VGYAVMNLAVNFWLTNKGILPTTDAAVLKANNIQSILGIQSIDTGILGAVIAGIIVWM
                     LHERFHNIRLPDALAFFGGTRFVPIISSLVMGLVGLVIPLVWPIFAMGISGLGHMINS
                     AGDFGPMLFGTGERLLLPFGLHHILVALIRFTDAGGTQEVCGQTVSGALTIFQAQLSC
                     PTTHGFSESATRFLSQGKMPAFLGGLPGAALAMYHCARPENRHKIKGLLISGLIACVV
                     GGTTEPLEFLFLFVAPVLYVIHALLTGLGFTVMSVLGVTIGNTDGNIIDFVVFGILHG
                     LSTKWYMVPVVAAIWFVVYYVIFRFAITRFNLKTPGRDSEVASSIEKAVAGAPGKSGY
                     NVPAILEALGGADNIVSLDNCITRLRLSVKDMSLVNVQALKDNRAIGVVQLNQHNLQV
                     VIGPQVQSVKDEMAGLMHTVQA"
     gene            21375..22547
                     /gene="malY"
                     /locus_tag="ACHKBL_001372"
     CDS             21375..22547
                     /gene="malY"
                     /locus_tag="ACHKBL_001372"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional maltose regulon transcriptional
                     repressor/cystathionine beta-lyase MalY"
                     /protein_id="HFO5818263.1"
                     /translation="MFDFSKVVDRHGTWCTQWDYVADRFGTADLLPFTISDMDFASAP
                     CIIEALNQRLMHGVFGYSRWKNDEFLAAIAHWFSTQHYTAIDTQSVVYGPSVIYMVSE
                     LIRQWSEKGEGVVIHTPAYDAFYKAIEGNQRTVMPVALEKRADGWFCDMGKLEAVLAK
                     PECKIMLLCSPQNPTGKVWTCDELEIMADLCERHGVRVISDEIHMDMVWGEQPHIPWS
                     NVARGDWALLTSGSKSFNIPALTGAYGIIENSSSRDAYLSALKGRDGLSSPSVLALTA
                     HIAAYQQGAPWLDALRVYLKDNLTYIADKMNAAFPELNWQIPQSTYLAWLDLRPLNID
                     DNALQKALIEQEKVAIMPGYTYGEEGRGFVRLNAGCPRSKLEKGVAGLINAIRAVR"
     gene            22651..23652
                     /gene="add"
                     /locus_tag="ACHKBL_001373"
     CDS             22651..23652
                     /gene="add"
                     /locus_tag="ACHKBL_001373"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009168 - purine ribonucleoside
                     monophosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HFO5818264.1"
                     /translation="MIDTTLPLTDIHRHLDGNIRPQTILELGRQYNISLPAQSLETLI
                     PHVQVIANEPDLVSFLTKLDWGVKVLASLDACRRVAFENIEDAARNGLHYVELRFSPG
                     YMAMAHQLPVAGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHR
                     DQITALDLAGDELGFPGSLFLSHFNRARDAGWHITVHAGEAAGPESIWQAIRELGAER
                     IGHGVKAIEDRALMDFLAEQQIGIESCLTSNIQTSTVAELAAHPLKTFLEHGIRASIN
                     TDDPGVQGVDIIHEYTVAAPAAGLSREQIRQAQINGLEMAFLSAEEKRALREKVAAK"
     gene            complement(23688..24728)
                     /gene="ydgJ"
                     /locus_tag="ACHKBL_001374"
     CDS             complement(23688..24728)
                     /gene="ydgJ"
                     /locus_tag="ACHKBL_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFO5818265.1"
                     /translation="MSDNIRVGLIGYGYASKTFHAPLIAGTPGLELAVISSSDETKVK
                     ADWPTVTVVSEPKHLFNDPNIDLIVIPTPNDTHFPLAKAALEAGKHVVVDKPFTVTLS
                     QARELEALAKSLGRVLSVFHNRRWDSDFLTLKGLLAEGVLGEVAYFESHFDRFRPQVR
                     DRWREQGGPGSGIWYDLAPHLLDQAITLFGLPVSMTVDLAQLRPGAQSTDYFHAILSY
                     PQRRVILHGTMLAAAESARYIVHGSRGSYVKYGLDPQEERLKNGERLPQEGWGYDMRD
                     GVLTRVEGEERIEETLLTVPGNYPAYYAAIRDALNGDGENPVPASQAIQVMELIELGI
                     ESAKHRATLCLA"
     gene            24971..25096
                     /gene="blr"
                     /locus_tag="ACHKBL_001375"
     CDS             24971..25096
                     /gene="blr"
                     /locus_tag="ACHKBL_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division septum protein Blr"
                     /protein_id="HFO5818266.1"
                     /translation="MNRLIELTGWIVLVVSVILLGVASHIDNYQPPEQSTTVQHK"
     gene            25369..25584
                     /gene="ydgT"
                     /locus_tag="ACHKBL_001376"
     CDS             25369..25584
                     /gene="ydgT"
                     /locus_tag="ACHKBL_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription modulator YdgT"
                     /protein_id="HFO5818267.1"
                     /translation="MTVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAAD
                     HRRAELVSGGRLFDLGQVPKSVWHYVQ"
     gene            25670..26110
                     /gene="ydgK"
                     /locus_tag="ACHKBL_001377"
     CDS             25670..26110
                     /gene="ydgK"
                     /locus_tag="ACHKBL_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HFO5818268.1"
                     /translation="MTTTTPQRIGGWLLGPLAWLLVALLSTTLALLLYTAALSSPQTF
                     QTLGEQALTTQILWGVSFITAIAMWYYTLWLTIAFFKRRRCVPKHYIIWLLISVLLAV
                     KAFAFSPVEDGIAVRQLLFTLLATALIVPYFKRSSRVKATFVNP"
     gene            26187..26768
                     /gene="rsxA"
                     /locus_tag="ACHKBL_001378"
     CDS             26187..26768
                     /gene="rsxA"
                     /locus_tag="ACHKBL_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HFO5818269.1"
                     /translation="MTDYLLLFVGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLA
                     TTFVMTLASICAWLIDTWILIPLNLIYLRTLAFILVIAVVVQFTEMVVRKTSPVLYRL
                     LGIFLPLITTNCAVLGVALLNINLGHNFFQSALYGFSAAVGFSLVMVLFAAIRERLAV
                     ADVPAPFRGNAIALITAGLMSLAFMGFSGLVKL"
     gene            26768..27346
                     /gene="rsxB"
                     /locus_tag="ACHKBL_001379"
     CDS             26768..27346
                     /gene="rsxB"
                     /locus_tag="ACHKBL_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HFO5818270.1"
                     /translation="MNAIWIAVAAVSLLGLAFGAILGYASRRFAVEDDPVVEKIDEIL
                     PQSQCGQCGYPGCRPYAEAISYNGEKINRCAPGGEAVMLKIAELLNVEPQPLDGEAQE
                     LTPARMVAVIDENNCIGCTKCIQACPVDAIVGATRAMHTVMSDLCTGCNLCVDPCPTH
                     CISLQPVAETPDSWKWDLNTIPVRIIPVEHHA"
     gene            27339..>29119
                     /gene="rsxC"
                     /locus_tag="ACHKBL_001380"
     CDS             27339..>29119
                     /gene="rsxC"
                     /locus_tag="ACHKBL_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HFO5818271.1"
                     /translation="MLKLFSAFRKNKIWDFNGGIHPPEMKTQSNGTPLRQVPLAQRFV
                     IPLKQHIGAEGELCVSVGDKVLRGQPLTRGRGKMLPVHAPTSGTVTAIAPHSTAHPSA
                     LAELSVIIDADGEDCWIPRDGWADYRSRSREELIERIHQFGVAGLGGAGFPTGVKLQG
                     GGDKIETLIINAAECEPYITADDRLMQDCAAQVVEGIRILAHILQPREILIGIEDNKP
                     QAISMLRAVLADSHDISLRVIPTKYPSGGAKQLTYILTGKQVPHGGRSSDIGVLMQNV
                     GTAYAVKRAVIDGEPITERVVTLTGEAIARPGNVWARLGTPVRHLLNDAGFCPSADQM
                     VIMGGPLMGFTLPWLDVPVVKITNCLLAPSANELGEPQEEQSCIRCSACADACPADLL
                     PQQLYWFSKGQQHDKATTHNIADCIECGACAWVCPSNIPLVQYFRQEKAEIAAIRQEE
                     KRAAEAKARFEARQARLEREKAARLERHKSAAVQPAAKDKDAIAAALARVKEKQAQAT
                     QPIVIKAGERPDNSAIIAAREARKAQARAKQAELQQTNDAATVADPRKTAVEAAIARA
                     KARKLEQQQANAEPEEQVDPRKAAVEAA"
BASE COUNT         7017 a         7547 c         7159 g         7396 t
ORIGIN      
        1 ttgatgccgc ctttcgcatt tttagtcaca aatttccccg cgaccattgc atgcagacac
       61 atctcttcgt aaaatcccgc gcttcagtgg caaactccat ttttatgcgt tttgccctaa
      121 tatttattct tcttgtcacg ttttaatcac tggatatcga tggaaaagaa actgggactg
      181 agcgcactca ccgcgctggt attaagctca atgctgggcg cgggtgtttt cagtctgccg
      241 caaaatatgg cggcagttgc cagcccggca gcactgctca tcggctggag tattactggt
      301 gctggtattt tattactgac ctttgccatg ctgatcctca cgcgcattcg tcccgaactt
      361 gacggcggta tctttaccta tgcccgcgaa ggatttggtg agctaatcgg cttttgttcc
      421 gcatggggat actggctgtg cgcggtcatc gccaacgtct cctatctggt gatcgttttt
      481 tccgcgttaa gcttttttac ggacacgccg gaattgcgcc tgtttggcga tggtaatacc
      541 tggcaatcga tcgtcggtgc atcggcttta ttgtggattg ttcacttttt gatcctgcgc
      601 ggagtgcaaa ccgctgccag cattaacctg gtggcgacac tggcaaaatt gttgccgctg
      661 ggtctgtttg ttgtgctggc gatgatgatg ttcaaactgg ataccttcaa gctcgacttc
      721 accggacttg cacttggcgt acccgtttgg gaacaggtga aaaacaccat gctgatcacc
      781 ctgtgggttt tcattggtgt ggaaggtgcc gtggttgttt ctgcgcgtgc gcgtaataaa
      841 cgtgatgttg gcaaagcgac actgctggcg gttctctccg ctctgggcgt ttacttgctg
      901 gtaacgctgc tttcgctggg cgtggtggca cgtcctgaac tggctgaaat tcgtaacccg
      961 tcaatggccg gactgatggt ggaaatgatg ggaccgtggg gcgaaatcat catcgctgcc
     1021 ggtttgatcg tttccgtttg cggtgcgtac ttaagctgga ccatcatggc ggcagaagtt
     1081 ccgttcctgg cagccactca taaagcattc ccgcgcattt ttgcgcgcca gaacgcacaa
     1141 gctgcgccat ccgcttcact gtggctgacc aatatctgcg tgcaaatttg tctggtactc
     1201 atctggctga ccggttccga ttacaacaca ttgctgacca tcgcctcaga aatgattctg
     1261 gtgccctatt tcctggtcgg cgcgttcttg ctgaaaatcg caacccgtcc actgcataaa
     1321 gcggtaggtg tcggtgcctg catttatggc ttatggttat tgtacgcctc tggtccaatg
     1381 cacttgctgt tatccgttgt tctgtatgca ccggggctgc tggttttcct gtacgcgcgt
     1441 aaaacgcata cccatgataa tgtactgaat cgtcaggaaa tggtgctgat tggcatgctg
     1501 ttaattgcct cagttccggc gacctggatg ctggtgggat aacgttcatc ccatcgttgg
     1561 atagcaaagg agataacgat gggtaaagcc caacccttgc caatattaat tactggcgga
     1621 ggtcgtcgca tcggcctcgc cctcgcctgg catttcatta atcaaaagca accggtgatt
     1681 atcagctatc ggacgcacta tcccgccatt gatggactga ttaacgcagg tgcgcagtgt
     1741 attcaggctg atttttcgac caacgacggt gtgatggcgt ttgccgatga agtactaaaa
     1801 agcacccatg gcctgcgtgc tattttgcat aacgccagcg cgtggatggc agaaaaacca
     1861 ggtacaccac tgaccgacgt tctggcttgc atgatgcaga tccacgttaa taccccatac
     1921 ctactcaacc atgcactgga aagattactg cgtgggcacg gacacgccgc cagcgatatc
     1981 attcacttta ccgattatgt ggtggagcgc ggtagcgaca aacatattgc ctatgctgca
     2041 agcaaagcgg cactggataa tatgacccgc tcgtttgccc gcaagctggc accggaagtg
     2101 aaagtgaatt ctattgcgcc atcgctgatc ctgtttaatg agcatgatga tgccgaatac
     2161 cgacaacagg cgctgaataa atcactgatg aaaaccgcgc ctggcgagaa agaagtgatc
     2221 gacctggtcg attacttact taccagctgc tttgtcaccg gacgcagttt ccctcttgat
     2281 ggcggtcgtc atctgcgtta atgcagcttt atccagcata taatcaacac ccatgcgctt
     2341 atcccccagc atgcaacaga accaacaaaa gcggcgggca agcgcatcat tccggtgaaa
     2401 taccacagcg acgccaggta aacaaaatag ggaataatcg accacatgct aaaaatgatg
     2461 gttgcgcgta aggcttcaat gccgcgttcg ctggcaacaa tataatgcgc gatgagcgca
     2521 aaggtcggga aaagtggtat cagcccggcg atataataat ttttcgtttt tgctaaaaca
     2581 ccaatcaaca gcactaccag cgcaccgagc gcggctttga ttaccagccc catcttttta
     2641 ccttaacact tccataacaa gttatcagta gaatacctga tgaaaccttg tttagaaacg
     2701 attgatagta agtaaaaaca gcgcggtgta ttgtgacgtt tttatatcta ccgtgaatgt
     2761 tatgaacact atcgtatttg tggaagatga tgcggaagtc ggttcactga ttgccgcgta
     2821 cctggcaaaa catgatatgc aggttaccgt ggaaccgcgc ggcgaccggg ccgaagaaac
     2881 tattctgcaa gaaaatccgg atttggtgtt actcgacatc atgctaccag gcaaggacgg
     2941 catgaccatt tgtcgtgatt tacgcgcaaa gtggtccgga ccgattgttc ttctaacctc
     3001 tctcgatagc gatatgaacc acatcctggc actggaaatg ggtgcctgcg actatattct
     3061 caaaacgacg ccccctgctg ttttgttagc gcgtttacgt ttgcatttgc gtcagaatga
     3121 gcaagccaca ctgagcaaag gtcttcagga aacgtctctg actccctaca aagccctgca
     3181 tttcggcacg ttgaccatcg atcctatcaa ccgcgtagtc accctggcta acactgaaat
     3241 ctcgctctcg acagctgatt tcgaattatt gtgggaatta gctacccatg ctgggcaaat
     3301 catggaccgc gatgcattgc tgaaaaattt acgcggcgtc agttatgacg gactggatcg
     3361 tagcgtggac gtggctattt cgcggttaag aaaaaaactg ctcgataacg ccgcagaacc
     3421 ttatcgcatt aaaactgttc gtaacaaagg ctatcttttt gcgcctcatg catgggaata
     3481 agcgatgaaa aaactgttta tccagtttta cctgttattg tttgtctgct tccttgtgat
     3541 gtctctgctg gttgggctgg tgtacaaatt taccgccgaa cgcgcgggca aacagtcgct
     3601 ggatgatttg atgaacagtt cgctgtatct gatgcgcagc gaattgcgtg agatcccccc
     3661 acacgattgg ggtaagacgc tgaaggagat ggatttaaat ctctctttcg atctgcgagt
     3721 cgagccactg agtaaatacc atcttgatga tatttccatg caccggctgc gtggcggcga
     3781 aattgtcgcc ctggacgatc agtacacttt tttgcaacgt atcccgcgca gccactacgt
     3841 gctggcagtt ggtcctgttc cttatcttta ttacctccat cagatgcgat tactggatat
     3901 cgccctgatc gcttttattg ccatttccct cgcctttccg gtgtttattt ggatgcgtcc
     3961 gcactggcag gatatgttaa aactggaagc agcggcgcaa cgatttggcg atgggcatct
     4021 caatgaacgt atccactttg atgaaggttc gagctttgaa cgacttggcg tcgcatttaa
     4081 ccagatggcg gacaatatca acgccttgat tgccagcaaa aaacagctta ttgacggtat
     4141 cgctcacgaa ctgcgaacac cgttagtgcg cctgcgttat cgactggaga tgagcgataa
     4201 cctgagcgcc gtcgaatccc aggcgttgaa tcgcgatatc agtcaacttg aagctttaat
     4261 tgaagagctg ctgacttatg cccgactcga tcgcccacaa aatgagcttc atcttagcga
     4321 accagacctg ccgttgtggc tgtcaacgca tctggcagat attcaggcag taacgcccga
     4381 taaaacggta cggataaaaa cgctcgcgca aggccattat gctgcgttgg atatgcgctt
     4441 aatggagcgc gtgctggata atttgctcaa taacgccctg cgctactgtc actcaacggt
     4501 tgaaaccagc ctgctactgt cggggaatag agcgacatta attgttgagg atgatggccc
     4561 agggattgcc cctgaaaacc gcgaacatat ctttgaacct tttgttcgcc tcgatcccag
     4621 ccgggatcgc tcaaccggcg gctgcgggct ggggctggca attgtccact ctatagcact
     4681 ggcaatgggc ggtacggtta attgtgacac cagcgaactg ggtggtgccc gcttctcgtt
     4741 tagctggccg ttatggcata acatcccgca atttacctct gcttgacatt acgcgcacgg
     4801 tggtcaagtc accaccactg tgctataaaa taaggatgtt gtaactaaag tggttaatat
     4861 tatggcgcgt tacgatctcg tagaccgact caacactacc tttcgccaga tggaacaaga
     4921 gctggctgca tttgccgctg agctagaaca gcacaaattg ctggttgccc gcgtgttctc
     4981 tttgccggag gtaaaaaaag aggatgagca taatccgctt aatcgtattg aggtaaagca
     5041 acatctcggc aacgacgcgc agtcgctggc gttacgtcat ttccgccatt tatttattca
     5101 acaacagtcc gaaaatcgca gcagcaagac cgctgttcgt ctacctggcg tgttgtgtta
     5161 ccaggtcgat aacctttcgc aagcagcgtt ggtcagtcat attcagcaca tcaatcaact
     5221 caagactacg ttcgagcata tcgtcacggt tgagtcagaa ctccccacag cggcgcgttt
     5281 tgaatgggtg catcgtcatt tgccggggct gatcaccctt aatgcttacc gaacgctcac
     5341 tgttctgcac gatcccgcca ctttacgttt tggctgggcc aataaacata tcattaagaa
     5401 tttgcatcgt gatgaagtcc tggcacagct ggaaaaaagc ctgaaatcac cgcgcagtgt
     5461 cgcaccgtgg acgcgcgagg agtggcaaag aaaactggag agagagtatc aggatattgc
     5521 taccctgcca caggacgcga agttaaaaat caaacgtccg gtgaaggtgc agccgattgc
     5581 ccgcgtctgg tacaagggag atcaaaaaca agtacaacac gcttgcccta caccgcttat
     5641 tgcgctgatt aatcgggata atggcgcggg cgtgccggac gttggggagt tgttaaatta
     5701 cgatgccgac aatgtgcaac accgttataa accacaggcg cagccgcttc gtttgatcat
     5761 tccacggctg cacctgtatg ttgcagatta acccccggct ttcatactgc cgaccatctg
     5821 ttctggccgt acccagctgt caaactcggc ttcgctaaga taccccagcg caagggccgc
     5881 agcttttaag gtcagccctt ctttatgcgc ttttttggcg atctcggcgg ctttgtcata
     5941 accaatgtgg gtgttaagcg cagtcaccag catcagcgat tcattgagta attgattgat
     6001 tcgctcacga ttcggttcaa tgcccactgc gcagtgtttg ttaaaacttt ccatgccatc
     6061 tgccagcaag cgcaccgatt gcaggaaatt atggatcacc atcggacgga agacgttcag
     6121 ttcaaagtta ccggaagcgc cacccatgtt gatcgccaca tcgttcccca tcacctgaca
     6181 gcagagcatg gttaatgctt cacactgcgt cgggttcact ttccccggca tgattgagct
     6241 acccggctca ttttccggga ttgagatttc accaattccg cagcgcgggc cagaggccag
     6301 ccagcggaca tcattggcaa ttttcatcag tgacgcagcc aaccctttca acgcgccgtg
     6361 cgcctgaacc agggcatcac aggtcgccag cgcttcaaat ttgttcggcg cggtaacaaa
     6421 cggtgcacaa gtaatgactg ccagttcatc tgccacgcga cgcgcatact ccggatgggt
     6481 atttagtcca gtacccaccg ctgtaccgcc aagagccagt tccgctacat gcggcaggct
     6541 gtattcgata tgtttcagat tatgctcgag catcgctacc cagccggaaa tctcctgccc
     6601 cagcgttaac ggcgtggcgt cctgcaagtg ggtacgaccg attttgacga tatcagcaaa
     6661 tacgcgggat ttttcactca gcgtctgggt cagggtttta agttgtggaa tgagttgctt
     6721 gcgcagcgcc agtagtgccg caacgtgcat cgccgtcgga aagacatcgt tggaactttg
     6781 gcttttgttc acgtcgtcgt tagggtgaac tttacgttcc atcccgcgca cgccaccgag
     6841 taattcactg gcccggttag ccagcacttc gttcatgttc atgttacttt gcgtgccgga
     6901 gccggtctgc cagatagcca gcgggaattc gtcgtcatgc tgtcctgcca gtacttcatc
     6961 cgccgcctgc cgaatggcgc tcgctttctc ttcagacaac aagcctaaat cttcattaac
     7021 ttttgccgct gcgcgcttgg ttagcgccag cgcatgaatc agtgaggtgg gcattttctc
     7081 cgtcgaaatg cggaaatgct ccagcgagcg ctgagtttgt gcgccccaca gcttatctgc
     7141 cgggacatca atcgctccca tcgaatcttt ttcgctgcgt actgtattca tggcctgctc
     7201 ctcacctgat taattttttc tttctgtttt gctttcgtta agcaactttt agcttactca
     7261 ttatttacca tttgataaca aatgtttggt ctttcgtgcc atgtaaaaaa accgccccga
     7321 agggcggctc tgtttatttc acacagcggg tgcactgtgt gagttgtatc tgctggaaga
     7381 agtcatttcc tttatcatcc acaaggataa atgccgggaa atcttccact tcaattttcc
     7441 agatggcttc cattcccagt tccggatatt caacacattc caggctctta atgcttccct
     7501 gcgccaatac agctgccgga ccaccgatac tgccaaggta gaagccgccg tgttttttac
     7561 aggcatccgt cacctgctgg ctgcggttgc ctttcgccag catgatcata cttccgccct
     7621 gcgcttgcag ttgatcgaca taagaatcca tccgtccggc ggtcgttggg ccaagagaac
     7681 cggaggcata accttccggc gttttggccg gacccgcgta gtaaatcgga tgatctttaa
     7741 tatactgcgg cagcccttcg ccgttatcca tccgctcttt cagtttggcg tgagcaatat
     7801 cacgaccaac gataatcgtg ccattcagcg ataagcgtgt agaaacggga tactgcgaca
     7861 actgtgcgag gatctctttc atcggacggt taaggtcaac gcgcaccgct tcgccttctc
     7921 ccgctttacg cagctcttcc gggatatatt tgcctggatt atgttccagt ttttcgatcc
     7981 agatcccctg acggttgatc ttcgctttga tattacggtc agcagagcag gagacgccca
     8041 taccgaccgg gcaggatgcg ccgtgacgtg gcaggcgaat cacgcggatg tcgtgagcga
     8101 agtatttacc accaaactgc gcacccagac caagattttg cgcttcgatc agcaattctt
     8161 tttccagttc cacatcgcgg aacgcctgac cgtgctcatt cccttccgtt ggcagttcgt
     8221 catagtattt agcggaagcc agtttcaccg ttttaaggtt cgtttccgca gaagttccac
     8281 caattacgaa cgcaatatga tacggaggac aggccgccgt acccagcgtg cgcatcttct
     8341 caaccaggta atttttcagt ttccccggcg ttagtaacgc tttggtttcc tgatagagat
     8401 acgtcttgtt tgccgaacca ccacctttgg cgatacagag gaatttgtac tcgtcgccat
     8461 caacggcata aagatcgatc tgcgctggta gattggtgcc ggtattcacc tctttataca
     8521 tatccagcgg cgcgttttgc gagtagcgca gattatcttc gatataagtg ttatagacac
     8581 cgcgtgccag cgccgcttca tcaccgccac cggtccatac acgctgccct tttttgccaa
     8641 caataatcgc ggtgccggta tcctgacagg ttggcagaac gcctttcgcc gcgatgtcgg
     8701 agttgcgcag gaattgcagc gccacatatt tatcattttc gctggcttcc gggtcacgca
     8761 gaatgtcggc cacctgttgt tggtgagccg gacgcagcat aaatgacgca tcatgaaacg
     8821 cctgacgcgc caacaaagtt aacgcttcgg gtgcgacttt caaaatctcc tgcccttcaa
     8881 attcagatac gctaacgtgt tcgctggtta gcaggtaata ctcagtatca tcttttttga
     8941 gtggaaaagg agcctgataa tgaaagggtt tgtttgacat tgttctctca cttactgcct
     9001 ggtttggtta tgctctgggc gggtgttccg ttgccctgtt aaaagcgagt aacaatatcc
     9061 tacacacttt tttaacaaaa actgagacta gtacgacttt ttgcggctcc aggttacttc
     9121 ccgtaggatt cttgctttaa tagcgggatt aatttccaca ttaaaacagg gattgatcat
     9181 gcaaaaactc attaactcag tgcaaaacta tgcctggggc agcaaaacgg cgttgactga
     9241 actttatggt atggaaaatc cgtccagcca gccgatggcc gagctgtgga tgggcgcaca
     9301 tccaaaaagc agttcacgag tgcagaatgc cgccggagat atcgtttcac tgcgtgatgt
     9361 gattgagagt aataaatcga ctctgctcgg agaggccgtt gccaaacgct ttggcgaact
     9421 gcctttcctg ttcaaagtat tatgcgcagc acagccgctc tccattcagg ttcatccaaa
     9481 caaacgcaat tctgaaatcg gttttgccaa agaaaatgcc gcaggtattc cgatggatgc
     9541 cgccgagcgt aactataaag atcctaacca caagccggag ctggtttttg cgctgacgcc
     9601 tttccttgcg atgaacgcgt ttcgtgaatt ttccgagatt gtctccctgc tccagccagt
     9661 cgcaggtgca catccggcga ttgctcactt tttacaacag cctgatgccg aacgtttaag
     9721 cgaactgttc gccagcctgt tgaatatgca gggtgaagaa aaatcccgcg cgctggcgat
     9781 tttaaaatcg gccctcgaca gccagcaggg tgaaccatgg caaacgattc gtttaatttc
     9841 tgaatgttac ccggaagaca gcggcctgtt ctccccgctg ttgctgaatg tggtgaaatt
     9901 gaatcctggc gaagcgatgt tcctgttcgc tgaaacaccg cacgcttacc tgcaaggcgt
     9961 ggcgctggaa gtgatggcga actccgataa cgtgctgcgt gcaggtctga cgcctaaata
    10021 cattgatatt ccggaactgg ttgccaacgt gaagttcgaa gccaaaccgg ctaaccagtt
    10081 gttgacccag ccggtaaaac aaggtgcaga actggacttc ccgattcctg tggatgattt
    10141 tgccttctcg ttgcatgacc ttagtgataa agaaaccacc atcagccagc agagtgccgc
    10201 cattttgttc tgcgtcgaag gcgatgcaac gctgtgtaaa ggttctcagc agttacagct
    10261 taaaccgggt gaatcagcgt ttattgccgc caacgaatca ccggtgactg tcagaggcca
    10321 cggccgttta gcgcgtgttt acaacaagct gtaagagctt actgaaaaaa ttaacatctc
    10381 ttgctaagct tattaaaggc ttataacacc ttcaggcggc cagtccgcct gatttcattt
    10441 tatggataat cattatgaat aaatcgctgg tagcggtagg cgtcattgtt gcgctaggcg
    10501 tagtctggac aggcggcgca tggtatacag gcaagaagat tgaaacccat ctcgaagaca
    10561 tggtcgcgca ggcgaacgcg caactcaaac tgaccgctcc tgaatccaac ctggaagtga
    10621 gttatcaaaa ctatcatcgc ggcgtattca gcagtcagct gcaactgttg gtgaaaccca
    10681 ttgccggaaa agtaaatccg tggattaaaa gcggtcagag cgtcatcttc aacgaatcgg
    10741 ttgatcatgg tcccttcccg cttgcccagc ttaaaaaact gaatctgatc ccgtcgatgg
    10801 catcaattca aaccacgctg gttaataacg aagtaagcaa accactgttt gatatggcaa
    10861 aaggtgaaac gccttttgag attaactccc gcattggtta cagcggagat tccagttccg
    10921 acatttcgct caagccactg aattacgagc aaaaggatga aaaagtcgcc tttagcggcg
    10981 gcgagttcca gttaaatgca gacagagacg gcaaagctat ctccctttcc ggggaggcgc
    11041 aaagtggtcg gatagacgct gttaacgaat acaaccagaa agtacagttg acctttaata
    11101 atctgaaaac cgacggttcc agcacgctgg caagttttgg tgagcgcgta ggaaaccaaa
    11161 aactgtcact ggaaaaaatg accatttcag tggaaggcaa agaactggca ctgctggaag
    11221 gcatggagat cagcggtaaa tcggatctgg tcaatgatgg taaaacgatc aatagccaac
    11281 tggattactc gctaaacagc ctgaaggtac agaatcagga tctgggcagc ggcaagctga
    11341 ctttaaaagt cggccaaatt gatggcgaag cctggcatca gtttagccag caatataacg
    11401 cgcaaactca ggcgctgctg gcacagccag aaattgccaa caatcccgaa ctttatcagg
    11461 aaaaagtgac ggaagccttc tttagcgccc tgccgctgat gttgaaaggc gatccggtga
    11521 ttactatcgc gccgctaagc tggaaaaaca gtcagggtga aagtgcgctg aatctgtcgc
    11581 tgttcctgaa agatccggca acgactaaag aagcgccgca aacgctggcg caggaagtag
    11641 accgttcggt taaatctctg gatgcgaaac tgaccattcc ggtggatatg gcaactgagt
    11701 tgatgactca ggtagcgaag ctggaaggtt atcaggaaga tcaagcgaaa aaactggcga
    11761 aacagcaagt tgaaggtgca tcagcaatgg ggcagatgtt ccgtctgacc accttgcagg
    11821 acaataccat caccaccagc ctgcaatatg ctaacggtca gatcacgtta aacgggcaga
    11881 aaatgccact ggaagatttt gttgggatgt ttgcgatgcc ggcattaaac gttccggctg
    11941 tacccgctat tccgcagcag taattcacag ccaaaatgcc ggagattttt ctctccggca
    12001 ttttttctta cttcagcata aagtcatact gaatactcat tttcacatcc cagtaattca
    12061 ggcgatcgcc cagcgcactg ccatcgcgct tttcccgccc tttatcggca gcaagaaaga
    12121 aattcatccc ttgtaacggc ccttcgctga cccgatagtt cataccacct aagatacccg
    12181 cgccttcaat ccgcacatct cgtttactgg attcatagcc gcgagtcaca atcggtgcaa
    12241 atgtcagcgt aaattgcggc gttaaacgat agttgacacc caattgccag gactgcattc
    12301 cttcacggtt acgatcaaga cttaatgaaa agggatagcc aatgtcggta tccaccacgg
    12361 caccattcat tgcatggcgc aaataaccac cactgccaaa tacgcttccc cacggcgcat
    12421 tccaggtcaa ttgtccacta accaacgccg tttcattggg ctggctggta ttacgactta
    12481 aaccttcaag ttgcgcgtaa aagccgagca actccccttt caacgtataa ttcgggtcaa
    12541 gcggtatgct tttctcgaga atactttgta tctgattgga aacatctttc tgtaccgatg
    12601 cccccagaga atagcgactg gtatcatcat gccaactggc ggctaaaaaa gaacgtgcgc
    12661 gatgtttatc gtattttccc gtctggctgt tatacacccc ttcatcacga ataccggttg
    12721 ttgaatcggt ccataaaaat actcgtgacc gccacattcc ccacagatca atatttggcg
    12781 ttaaagccca gtaacctaat gctccttcag aaactgtcgg taatgtacgt tgcgaactgc
    12841 cagccattaa cggaatacga taaggtaagc taccataact ataatccgtc ccgaagcgac
    12901 ctaattttag caggctattt tcactgggtt ttatttttac tgcccccagg gcaaagccaa
    12961 aacttttatc accatcaaga taccaccggg tactcatatc agcacgagca cctaatttat
    13021 aaacataata cgccccgcct tcaacgccaa taatattgga ataataacca ctattgaaat
    13081 cgagcaatcc tccctgtacc catgcgtaaa tatctcttcc cgtgccagca ctgggcttat
    13141 cagccctgcg taattcgttt ttcagtctga tgcgtagtcc cgcttcatcg tcagccggtt
    13201 gcgccgcgta agcagaggta aggccagagg cagccgtcag gcaaataacg gccatggcca
    13261 ctatttttct catagttgca cctttgatgt tctgattatt tttattgaat attaactagt
    13321 gatatcgttg attaattgct gctgaacttt tttacgatta tcaatttcaa caacgatttc
    13381 tttgaatttt ttatccgtga gcggataaaa ccagataatg acgaatgcca gtagcataaa
    13441 tccgcaaggt actaaggcaa ttgatgtgcg gatgcccata ataacttccg gcgtttgcgc
    13501 ctgattggcg atatatccgc ttaatcccaa aataaaggca ggaattgaac ctccgattgc
    13561 ctgaccgcat ttacgggtaa atgagaatag tgaataggtg agcccttcaa ttcgcacgcc
    13621 ggtcaggtat tcaccgtatt ctacggtatc agcttccagt gcccacatca cggtcatggt
    13681 aacgccctgg ccaattgatg cgatggccaa cgcaaccaac gccaccggca gtgaccagac
    13741 ggaaacccag aagaacagca gataaccgca ggttcccagc aaagcgccaa tcaggaaggt
    13801 attcttttta ccgatcctcg cgaccatccc cggcaccagc ggtgccgatg ccacagtacc
    13861 gaccaggttt tgcaccagta ccagcacagt gaacagcccg gtatcattta acacatagcg
    13921 cacgtagaac aacgacgagg cgctgaccgc gaaggtcgaa atcagcacac acagcgcacc
    13981 gatgcacaac ataaacagcg ggcgattccg tttcagggtt tgcagactga tcttcaatga
    14041 cggctgcgcc acgatacgta ccacattctc acgcgtcgat ttgaagcaga tgaagtaaag
    14101 caccattccg gcaatcgcca gcacaattgt ccagaaatga tataccgaca ccatctcttc
    14161 cgggctggag ttcttaatgc tcggtcctat cagaaatgcc aggcagacaa aagtcaatga
    14221 agcggcaatc ccgcgagccg cgcccagacg ggcgcgggat tgtggttgtt gggtcattgc
    14281 ggtagcaagt gaaccataag gaatattcac caggctgtag caaagcccga ggcccatgta
    14341 agtcaaatat gcatacacta ctttgctgct atggctccag tcggtcggta cccagaatac
    14401 cagcacgctg aagatcatta acggcgcagt accgaagagt aaaaacgggc ggaattttcc
    14461 ccatcgggta ttcacactgt ccaccactcg tccggcaaag acatcggcga aggcatcgaa
    14521 tacccgcacc agtaacagca tggtgcccgc cgcagcggca ccgacgccag cgacgtcggt
    14581 gtagtaactc aacaggaaga gcgcccccat tgcgaaggcg aagttattgg cgacgtcacc
    14641 gaggctgtag ccgacgatgg cgcgccagga gagttgttga ttcattgttt gcctccctgc
    14701 tgcggttttt caccgaagtt catgccggtc cagcgttttt gcagcagaaa agccgcagac
    14761 ttcggtttgc ggtcgcgagt gaagatccct ttcttgttac cgccaacgcg caatatgcct
    14821 tgcgaggtcg caaaatcggc gaaattccat acctgctcac cgacgacagc gctgacgcga
    14881 tcaaagacgc ggtgatacat atccagccat gcacactgat actcttcact ccacatgtcg
    14941 gtgtacattg agtgcaggcc ggctaacgta tccacaccgt attcggtgat gataatcggc
    15001 tgatgcagtt tctcctgcca ggccagaagt tctttttcca gaaccttctc tgccgtttcc
    15061 aaatcgccgc tttggacata ccaaccgtaa taacggttca ggcacagcac atcaaagaga
    15121 tcgctgatgg tatcggtgtg agcgtcgcag aacattacat tgacgcaggt gatcggacgc
    15181 gtcggatcga gtttacgcgt tgcttccgcc agtggcgcga aatattcccg tgcaccttgc
    15241 ggacgggtat ccggttcgtt ggcaatactc cacatcacca cgcttgggtg gtttttgtca
    15301 cgcgcaatca gctctttaat cgcctgtaag tgcgcctgct gggtttcccc gttgactgcc
    15361 tcttcgctgt acagttcttt cggcttgttg cccgcttcga aaccaatgcc taaagagagg
    15421 ttaaagccga cagctgcagt ttcatcaatc accacgatgc catgttcatc tgcccagtcg
    15481 agcatctctt cagcgtaagg gtaatgcgag gtacggtagg agttggcccc aatccagtcc
    15541 attagtgcgt gatcgtgcac catcagcacg ttatcgaatc ctttgccgcg caaatccgca
    15601 tcttcatgac gaccaaagcc agtaaagtag aacggtttgt ggttaatcag gaactgttcg
    15661 tccttcactg ccactgaccg gatgccgacg cgcagcgggt agatatcaca ctctgtctgg
    15721 cttttggctg tgacgcacag ttcatagaga taaccttcac ccggttgcca gaggtgcgga
    15781 ttcaccactt gcaaagtccc gctggtgcct tgtccagttg caaccacctg ctgatccgca
    15841 tcacgcagtt caacgctgac atcaccattt gctaccacct gccagtcaac agacgcgtgg
    15901 ttacagtctt gcgcgacatg cgtcaccacg gtgatatcgt ccacccaggt gttcggcgtg
    15961 gtgtagagca ttacgctgcg atggatcccg gcatagttaa agaaatcatg gaagtaagac
    16021 tgctttttct tgccgttttc atcggtaatc accattcccg gcgggatagt ctgccagttc
    16081 agttcattgt tcacacaaac ggtgatacgt acacttttcc cggcaataac atacggcgtg
    16141 acatcggctt caaacggcgt atagccaccc tgatgctcca tcacttcctg attatttacc
    16201 cacactttgc cgtaatgagt gaccgcatcg aaacgcagta cgatacgctg gcccgcccaa
    16261 cctttcggta taaagacttc gcgctgatac cagacgttgc ccacataatt acgaatatct
    16321 gcatcggcga actgatcgtt aaaacttccc ggcacagcaa ttgcccggat ttcttgtaac
    16381 acgctttccc accaacgctg atcaattcca cagttttcgc gatccagact gaatgcccac
    16441 agaccgtcga gttttttgat ttcacgggtt ggggtttcta caggacgtaa cataagggac
    16501 tcctcattaa gataaaaata ctggtcaacc tttaatctga ttagttaacc aattctgtgt
    16561 tgcacacagt tatctgtgag aaacccatgg aagagaaatg gaaataaaaa gaactgacac
    16621 cgggatcaaa aaaccaaaaa ctgcaatacc cacagcgtga tatagatcgc attaatcttt
    16681 aaaacggcgt tgacagaagc cataaaataa gcaaccgaac gttcgttcga gcataatggg
    16741 aacgataaac tctcatgaca tatggctaca gtgaatattt tggggggaat tctccgaacc
    16801 cgggaaggat aatttttgca cagagaaatt acgatatgaa acgcgggcac agttatcacc
    16861 agcccgcgaa atgagaggat caggatgcgg ttaagatacc gccaatcatc gcccgcaatc
    16921 cctgagcaaa cgacgcttca cgcgacagat cttccgcttc gatatcggcc agtacaccgt
    16981 aggtcagatc cagcagtaaa cgtgaagtca ttgccgtgtt gatgtctggg cttatctcgc
    17041 ctttttgctg ggcatcacgc atccgctggg caacaaattc cgtgatcgtc atatgcttat
    17101 ttttaagcat ggcggcgacc tgtgggttac gcatcccttc cgccataatt tcgaccacca
    17161 gcgcccgttg tccaaaggcc tcatgggtga gagagacaat agactcgacc atatagtcaa
    17221 cgaaatgaat cccttcaatc ggttcccgga aacgggccag cgccctctcc tggtcctgta
    17281 agataatcgc ctgaatcaag gcttctttgg agatgaaatg gtgatagagc gtcccgggac
    17341 taatggcgca agatttacag atggctttca tcgaggcact gtgaaatcca ttttctgaaa
    17401 aaatctctct ggcagcattg aggatccggg tccgtgtcgg ttgtgcttca gtctgcatgt
    17461 tatccatcat taaaccatta tagttagcgt gaataactag tttaccgcgt tgtcaggtga
    17521 gcgcctatca ttgctgttga atagtgcgaa ggcacactct attcgtatac ttttcactcg
    17581 gaaattggtt aaccaatagc cgtaatttgg ctgccagtta tttagctgag acgttgatga
    17641 gtttatcccg gtaaacaaca cgcacggttg tttaccggtc cgttagtgta ttaattgagc
    17701 tcctgtaccc caccaccgga gacggtgaga atttgtccgc ttacccagct ggctgcaggc
    17761 gagcaaagga acagcgccgc gttagcaata tcttgcggtt ggcccagacg tctgatcggc
    17821 gtgtgctgta acattttttg ttcaatttct ggtgtaataa cggatttcag ggcatcggtt
    17881 aatattgccc ccggcgcaat gccattcacc cgaatatttt tttcacccag gtcaaacgcc
    17941 atatttctga ccagatgact ggccgcagct ttagatgatg cataagaagt catgtttata
    18001 tttttatttt ctgccgccat agaagtgatg gtcagaataa cgccaccgcc atttttttcc
    18061 atttctggcg caacaagttg tgacagatgg aaaaaagaaa acacattcag ttcataagca
    18121 cggcgaaaat ctgccattgg catatcaaac ggtttaggac caccgccacc ggcgttgtta
    18181 accaggatat caactttacc cagcttactg acggcaaagt ctgccagtgc agagagttcc
    18241 tgttcggaag taatatcaca acggcaggca aatgcctgac cacccagttg ttgaatttca
    18301 tcaacaacat ggttagctgc gtcggcgtta atatcactga ccaccacaga tgcgccagct
    18361 gtcgcgaatg taatggcgat ttctttacca atacctgcac cggcacctgt gatgatggca
    18421 cattttccgt cgagtctcag gttgtcagaa ttaaacacat aaacctcctg tgaacttcag
    18481 taaaaataaa aggcaccgcc tgtgtgccac attttttatc attaaccttg aggaaaggac
    18541 actaccaggt atagcagata aagagaggat tatttcgccg caatgagccg ggcgggaata
    18601 ataagattgc gtgaatgcgt ctcttcatgg gtgatttttt gcatcatgcg atccgcaagt
    18661 gtggtaccaa gttcccgcgc aggcgtgctg gcccaggtaa caggtatatc atcaagtgtg
    18721 gttggcgttg catcggtaaa tgccgccaac gaaacctgtt gttcaaagta acgatcgact
    18781 ccgctttccc cgctttgccg ccctgctttc agcaaaccaa accatgcccc catcgcaata
    18841 gtttcgttat agcaaaccac agcactgatg gtcgggttat gacgtaaaag cgccgtgata
    18901 gcttccgcgg cttgcttctg gctggaagag cactccaaca cccaatcgct gtgaaacggc
    18961 aggccaaatt ttagtagagt tgcacaatag ccccccaccc gttctgcccg ggttaatgag
    19021 gaactttgcc ctcccagcca ggcgatacgc tgatgcccat tgcgaatgag atgctccgtc
    19081 aacaactgtg cagcctgcat gttatccggg cgaaccgtat caacatcatc aagataactg
    19141 gcacgggaag cgaaaatcac cgggatagct ttttcttctg ccatccgtcg caggtcatcg
    19201 ctgcttcctg cagccccggc aatgaccaca ccatcgaccc cctgattcag taacagtgaa
    19261 aaccgctgtg ccagctgctc gccgtcttta ccgccgtgaa gcaaaaaaac catccgtccc
    19321 tgcgcttcca gagcttccgt caatccggcc gttaattcgg cgtaaaacgg cgcagataaa
    19381 tcacggacga tcaaaccaat gacgccgctt tgcccgccgc gcagcgccga cgcctgacga
    19441 ttgcgcacaa atcccagctc ttcaatggcg gcgttaacgc gttctcctgt ggcggtagag
    19501 attcgccctt tgccacttag caccagcgaa acggtgctta ccgacacgcc cgcagccagc
    19561 gcaacatcat gaatggttat ttttttggcg gtagccatgc agatgaccta ctccctgatt
    19621 atgtgacaga taaaacgttt taccttttat ttatcttata cccgctatta tcgttgcgta
    19681 atgtgattta tgcctcacta aaatttgata aaacgtttta tcttctcgcg caatttactg
    19741 aatccagatt gttctctacg aggagtcgtt ttatgacggc gaaaacagca ccgaaagtca
    19801 cgctgtggga gttcttccag cagttaggca aaaccttcat gctacctgtg gcattattgt
    19861 cgttctgcgg cattatgctc ggcattggta gttctcttag cagccatgat gtcatcaccc
    19921 tgatcccggt cctgggcaac cccgtgttgc aggctatctt tacctggatg agtaagattg
    19981 gctcgtttgc ttttagtttc ctgccagtca tgttctgtat cgccatcccg ctgggcctgg
    20041 cacgcgaaaa caaaggtgta gcggcatttg ctggctttgt cggttatgcg gtaatgaatc
    20101 tcgcggtaaa cttctggttg accaataaag gcattctgcc aaccacggac gccgcggttc
    20161 tgaaagccaa taacattcag agcattcttg ggatccagtc gatcgataca gggatcctcg
    20221 gtgcggtgat cgccggtatt atcgtctgga tgctccatga gcgtttccat aatatccgcc
    20281 tgccggatgc gctggcattc ttcggcggta cgcgcttcgt gccaatcatc tcctcgctgg
    20341 tgatgggcct tgtcggtctg gtgattccat tagtctggcc aattttcgcc atgggtatta
    20401 gcggcttagg ccatatgatc aacagcgcgg gtgatttcgg accgatgctg tttggtaccg
    20461 gtgaacgtct gctgttgccg tttggtctgc atcacattct ggtggcatta attcgcttta
    20521 ccgacgcagg cggcacgcag gaagtctgcg gtcaaaccgt cagcggcgca ctgaccatct
    20581 tccaggcgca attgagttgc ccgaccactc acggtttttc tgaaagcgcc acgcgtttcc
    20641 tttcgcaagg taaaatgcct gcgtttctcg gcggcctgcc aggtgcagcg ttagctatgt
    20701 atcactgcgc gcgcccggaa aatcgccata aaattaaagg tctgcttatt tctggcctga
    20761 tcgcctgtgt cgttggcggc acgaccgaac cgctggaatt cctgttcctg ttcgtagcgc
    20821 cagttctgta tgtcatccac gcgctgttaa ccggcctcgg cttcaccgtc atgtctgtgc
    20881 tcggcgtcac catcggtaat accgacggca atatcatcga cttcgtggtg ttcggtattt
    20941 tgcatggtct gtcaaccaag tggtacatgg tgccagtggt ggcggcaatc tggtttgtcg
    21001 tttactacgt catcttccgt ttcgctatca cccgcttcaa tctgaaaacc ccggggcgcg
    21061 atagcgaagt tgccagctca atcgaaaaag ccgttgccgg tgcgccgggt aaatcaggtt
    21121 acaacgttcc ggcaatcctc gaagccttag gcggtgccga caatattgtc agcctcgata
    21181 actgcattac ccgtctgcgt ttgtctgtga aagatatgtc gcttgttaat gtgcaggcac
    21241 tgaaggacaa tcgggcaatt ggcgtagtac aacttaatca acataacctg caggttgtta
    21301 tcgggccaca agttcagtca gtaaaagatg aaatggccgg tctgatgcat actgtccagg
    21361 cataaggata agatatgttc gatttttcaa aggtcgtgga tcgtcatggc acatggtgta
    21421 cgcagtggga ttatgtcgct gaccgtttcg gcactgctga cctgttaccg ttcacgattt
    21481 cagacatgga ttttgcctct gccccctgca ttatcgaggc gctgaatcag cgcctgatgc
    21541 acggcgtgtt tggctacagc cgctggaaaa acgatgagtt tctcgcggct attgcccact
    21601 ggttttccac ccagcattac accgcaattg atacgcagtc ggtggtgtat ggcccttctg
    21661 ttatctacat ggtctcggaa ctgattcgtc agtggtctga aaaaggtgaa ggcgtggtga
    21721 tccacacacc cgcctatgac gcattttaca aggccattga aggtaaccag cgcacagtaa
    21781 tgcccgtcgc tttagagaag cgggctgacg gctggttttg cgatatgggc aagctggaag
    21841 ccgtgttggc gaaaccagaa tgtaaaatta tgctcctgtg tagcccacag aatcctaccg
    21901 ggaaagtgtg gacgtgcgat gaactggaga tcatggctga cctgtgcgag cgtcatggtg
    21961 tgcgggttat ttccgatgaa atccatatgg atatggtttg gggcgagcag ccgcatattc
    22021 cctggagtaa tgtggcgcgc ggagactggg cattgctaac gtcgggatcg aaaagtttca
    22081 atattcccgc cctgaccggt gcttacggga ttatagaaaa tagcagcagc cgtgatgcct
    22141 atttatcggc actgaaagga cgtgatgggc tttcttcccc ttcggtactg gcgttaactg
    22201 ctcatatcgc cgcctatcag caaggcgcac cgtggctgga tgccttacgc gtctatctga
    22261 aagataacct gacgtatatc gcagataaaa tgaacgccgc gttccctgaa ctcaactggc
    22321 agatcccgca atccacttat ctggcctggc ttgatctacg tccattgaat attgacgaca
    22381 acgcgttgca aaaagcgctt atcgagcaag agaaggtcgc gatcatgccg gggtatacct
    22441 acggtgaaga aggtcgtggt tttgtccgtc tcaatgccgg ctgcccgcgt tcgaaactgg
    22501 aaaaaggtgt tgctggatta attaacgcca tccgcgctgt tcgttaaccc caattgcgca
    22561 acgtaaaaaa tcgttgcgca atcgtggatt tttaccctgc tttgttttta taatggtgcg
    22621 cacttttata tccagaaaaa gagtgcgacc atgattgata ccaccctgcc attaactgat
    22681 atccatcgcc accttgatgg caacattcgt ccccagacca ttcttgaact tggccgccag
    22741 tataatatct cgcttcctgc acaatccctg gaaacactga ttccccacgt tcaggtcatt
    22801 gccaacgaac ccgatctggt gagctttctg accaaacttg actggggcgt taaagttctc
    22861 gcctctcttg atgcttgccg ccgcgtggca tttgaaaaca ttgaagatgc agcccgtaat
    22921 ggcctgcact atgtcgagct gcgtttttca ccgggctaca tggcaatggc acatcagctg
    22981 cctgtagcgg gtgttgtcga agcggtgatc gatggcgtac gtgaaggttg ccgcaccttt
    23041 ggtgtgcagg cgaagcttat cggcattatg agccggacct tcggcgaagc cgcctgtcag
    23101 caagagctgg aggccttttt agcccaccgt gaccagatta ccgcacttga tttagccggt
    23161 gatgaacttg gtttcccggg aagtctgttc ctttctcact tcaaccgcgc gcgtgatgcg
    23221 ggctggcata ttaccgtcca tgcaggcgaa gctgccgggc cggaaagcat ctggcaggcg
    23281 attcgtgaac tgggggcgga acgtattgga catggcgtaa aagccattga agatcgggcg
    23341 ctgatggatt ttctcgccga gcagcaaatt ggtattgaat cctgtctgac ctccaatatt
    23401 cagaccagca ccgtggcaga gctggcggca catccgctga aaacgttcct tgagcatggc
    23461 attcgtgcca gcattaacac tgacgatccc ggcgtacagg gagtggatat cattcacgaa
    23521 tataccgttg ccgcgccggc tgctgggtta tcccgcgagc aaatccgcca ggcgcagatt
    23581 aatggtctgg aaatggcttt cctcagcgca gaggaaaaac gcgcactgcg agaaaaagtc
    23641 gccgcgaagt aacaaaaatg gatggtgcta attgcaccat cctcttttca cgcaaggcac
    23701 agcgtcgcgc gatgtttggc ggattcgatg cccagctcaa tcaactccat tacctggatt
    23761 gcctgacttg ccggaaccgg attttcacca tcgccattta acgcatcacg aatagccgca
    23821 tagtaagccg gataattccc tggtactgtc aacagcgttt cttcgatacg ttcctcacct
    23881 tccacgcggg taagtacgcc atcacgcata tcgtagcccc agccttcctg cggcagacgc
    23941 tcgccatttt tcagtcgttc ttcctgtgga tcgaggccat atttcacata actgcctcgg
    24001 gatccatgca cgatataacg tgctgactca gcagctgcca gcatggtacc gtgtaaaatg
    24061 actcgccgct gcggatagga caagatggcg tggaaataat cggtcgactg cgctccgggc
    24121 cgtaactgtg ccaaatctac cgtcatgctg accggtaaac caaatagcgt aatggcctga
    24181 tcaagaaggt gcggtgctaa atcgtaccag ataccgctgc ctgggccgcc ctgttcacgc
    24241 caacgatcgc gaacctgcgg acggaagcgg tcaaaatgag actcaaagta agcaacttca
    24301 cccagcacgc cttccgcgag caaacctttt agcgtcagga aatcgctatc ccagcgacgg
    24361 ttatggaata cagacagcac acgccctagg ctttttgcca gcgcttccag ctctcgcgct
    24421 tgtgacagtg tcacggtaaa gggtttatca acgaccacat gtttacccgc ctcaagcgcc
    24481 gctttggcta acgggaaatg ggtatcgttg ggtgtaggaa tgacaatcag gtctatgttg
    24541 ggatcgttaa acagatgctt cggctcagag acaaccgtaa ccgttggcca gtcggctttt
    24601 acttttgttt catcgctgct ggagattacc gccagttcca gcccgggcgt gcccgcaatc
    24661 aggggcgcat ggaaggtttt gctcgcataa ccatacccaa tcaacccaac acggatgttg
    24721 tcgctcatgt cattgcctct cattgaagta tgatggctat ttgacactat cctttaccct
    24781 cgctcaacag tttaataacc tgccagcaat aagggatgtt gtttaactta agtcaaaaaa
    24841 atagcgaatt ttccaacgac aaaagctaaa tatcgcaaaa acctcagtaa aaatcttgct
    24901 ggagctatta ttgctaagta acatttaccc cctgaagtta atggatcaat caacagagat
    24961 gtgggctgta atgaatcgtc ttattgaatt aacaggttgg atcgttcttg tcgtttcagt
    25021 cattcttctt ggcgtggcga gtcacattga caactatcag ccacctgaac agagtactac
    25081 ggtacaacac aagtaagctc tgcgcttgta gagcgacatg ctgcccgtcc gggtgcatgt
    25141 tttcacttgt cggatattaa accaggaatt tattatcttg ttcgatgttg ttggtgattg
    25201 tcagagatag taaagcgcgt tactcttttc agagcagttt ttcatccctg aatatttgcc
    25261 atcagcgcat tcttattatt gtaataacac aagcgttcac ttttggtgaa attttatcat
    25321 cactaataat acctacccct ttggtattaa ttattttatg gaccttttat gactgttcag
    25381 gactacttat taaaatttcg caaaatcagt tcactcgaaa gtttggaaaa actctacgac
    25441 catcttaatt acaccctgac ggacgatcag gaactgatca atatgtatcg tgctgccgat
    25501 caccgtcgcg cagagctggt ttctggcggg cgtttgtttg acctcggcca ggtaccgaag
    25561 tccgtctggc actatgtcca ataaagaaag tagcgatcgg ttataaaagc cttataataa
    25621 cgcccctaaa attagatttg cctgtttgcg ccgctattat ggagaaccta tgaccaccac
    25681 aacgccacaa cgtattggag gctggttgct tggcccattg gcctggcttt tagtcgctct
    25741 gttaagtacg acgctggcgt tgttgctgta taccgctgcg ttatcttctc cgcaaacatt
    25801 tcaaacgctc ggcgaacaag cactgacgac acaaatctta tggggcgttt cttttattac
    25861 cgctatcgcc atgtggtatt acacactgtg gttgaccatc gctttcttca aacgtcggcg
    25921 ttgtgttcct aaacactata ttatctggct gcttatttcc gtattgctgg cagtaaaagc
    25981 gttcgctttt tcacccgtgg aagatggcat agccgttcgc cagttactgt ttactttgtt
    26041 ggcaaccgca ttgatcgtcc cctattttaa acgctcgtcg cgggtgaaag cgacgtttgt
    26101 gaatccgtaa taaccttaca gttaacctgt tgtcgcctgc tctggattaa cggataatag
    26161 gcggcttttt tatttcaggc cgaaaaatga ctgactacct gttactgttt gtcggaactg
    26221 tactggtcaa taactttgta ctggtcaagt ttctcggtct ctgtccgttt atgggggttt
    26281 ccaaaaagct ggaaaccgcg atgggcatgg ggctggctac aacgtttgtg atgacgctgg
    26341 cgtctatttg cgcctggctt atcgatacgt ggattttgat cccgcttaat ctgatttacc
    26401 tgcgcaccct ggcgtttatt ctggtgattg ctgtggttgt gcagttcacc gagatggtgg
    26461 tgcgcaaaac cagcccggtg ctttaccgtc tgctggggat ttttttgccg cttatcacca
    26521 ccaactgtgc agtgctcggc gtggcgttgc tgaatatcaa tctcgggcac aatttctttc
    26581 agtcggcgct gtacggtttt tccgccgctg tcggtttctc gctggtgatg gtgctcttcg
    26641 ccgccatccg cgaacgcctt gctgtggctg atgtcccggc accttttcgc ggtaatgcca
    26701 ttgcattaat taccgcaggt cttatgtctc tggcctttat gggctttagt ggtttggtga
    26761 agttgtaatg aatgctatct ggattgccgt tgccgccgtg agcctgctgg gcctggcgtt
    26821 tggcgccatt ctgggttatg cctcccgccg ttttgcggtg gaagacgatc cggtcgttga
    26881 gaaaattgac gaaatcttac cgcagagcca gtgtggtcag tgcggttatc ccggctgtcg
    26941 cccctacgcg gaagccatca gctataacgg tgaaaaaatc aaccgttgcg cccctggtgg
    27001 cgaagctgtg atgctaaaaa ttgccgagtt gcttaatgtc gagccgcagc cgctggatgg
    27061 cgaagcgcaa gagctaacgc ctgcgcggat ggtggcggtt attgatgaaa ataactgtat
    27121 tggctgcact aaatgtattc aggcttgtcc ggtagacgcc atcgttggcg ctacccgtgc
    27181 catgcatacg gtaatgagtg atctctgtac gggctgcaat ttatgtgttg atccgtgccc
    27241 gacgcactgc atctcgttgc aaccggtcgc agaaacacct gactcctgga aatgggatct
    27301 gaacaccatt cccgtgcgta tcattcccgt ggaacaccat gcttaagtta ttctctgcat
    27361 tcagaaaaaa taaaatctgg gatttcaacg gcggcatcca tccaccggag atgaaaaccc
    27421 agtccaacgg tacacccctg cgccaggtac cactggcgca gcgttttgtt attccactga
    27481 aacagcatat tggcgctgaa ggtgagttgt gcgttagcgt cggcgataaa gtattgcgcg
    27541 gccagccgct tacccgtggt cgcggcaaaa tgctgcctgt tcacgcgccc acctcgggta
    27601 ccgttacggc tattgcgccc cactctacgg ctcatccttc agcgttagct gaattaagcg
    27661 tgattattga tgccgatggt gaagactgct ggatcccgcg agacggctgg gccgattatc
    27721 gctctcgcag tcgcgaagag ttaatcgagc gcattcatca gtttggtgtt gccgggctgg
    27781 gcggtgcagg attcccgaca ggcgttaaat tgcagggtgg cggagataag attgaaacgt
    27841 tgattatcaa cgctgctgag tgcgagccgt acattaccgc cgatgaccgt ttgatgcagg
    27901 attgcgcggc tcaggtcgta gagggtattc gcattcttgc gcatattctg cagccacgcg
    27961 aaattcttat cggcattgaa gataacaaac cgcaggcgat ttccatgctg cgcgcggtgc
    28021 tggcggactc tcacgatatt tctctgcggg tgattccaac caaatatcct tctggcggtg
    28081 ctaaacaatt aacctacatt ctgaccggga agcaggttcc acatggcggg cgttcatccg
    28141 atatcggcgt attaatgcaa aacgtcggca ctgcttatgc agtgaaacgt gccgttattg
    28201 atggcgagcc gattaccgag cgtgtcgtaa ccctgactgg cgaagcaatc gctcgcccgg
    28261 gcaacgtctg ggcacggctg gggacgccag tgcgtcattt attgaatgac gccggattct
    28321 gcccctctgc cgatcaaatg gtgattatgg gcggcccgct aatgggcttt accttgccat
    28381 ggctggatgt cccggtcgta aagattacca actgtctgtt ggctccctct gccaatgagc
    28441 ttggcgaacc acaggaagaa caaagctgca tccggtgtag cgcctgtgct gacgcctgcc
    28501 ctgcggatct tttgccgcaa cagttgtact ggttcagcaa aggtcagcaa cacgataaag
    28561 ctaccacgca taacattgcc gactgcattg aatgtggggc ttgcgcgtgg gtttgcccga
    28621 gcaatattcc cctggtgcaa tatttccgtc aggaaaaagc tgaaattgca gctattcgtc
    28681 aggaagaaaa gcgcgccgca gaagccaaag cgcgtttcga agcgcgccag gctcgtctgg
    28741 agcgcgaaaa agcggctcgc cttgaacgac ataagagcgc agccgttcaa cctgcagcca
    28801 aagataaaga tgcgattgct gccgctctgg cgcgggtgaa agagaaacag gcccaggcta
    28861 cacagcctat tgtgattaaa gcgggcgaac gcccggataa cagtgcaatt attgcagcac
    28921 gggaagcccg taaagcgcaa gccagagcga aacaggcaga actgcagcaa actaacgacg
    28981 cagcaaccgt tgctgatcca cgtaaaactg ccgttgaagc cgctatcgcc cgcgccaaag
    29041 cgcgcaagct ggaacagcaa caggctaatg cggaaccaga agaacaggtc gatccgcgca
    29101 aagccgccgt cgaagccgc
//
LOCUS       DAZFLD010000037        28846 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.037, whole genome shotgun sequence.
ACCESSION   DAZFLD010000037 DAZFLD010000000
VERSION     DAZFLD010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28846
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.037"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(127..1251)
                     /locus_tag="ACHKBL_001381"
     CDS             complement(127..1251)
                     /locus_tag="ACHKBL_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001765453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoplasmic protein"
                     /protein_id="HFO5818272.1"
                     /translation="MSTLFTINACKTFGCRNLGLASSEDYSWPDYKLGYPALHCRACG
                     SYPPLFDEQQFRDWLSVHLSTWASEKGHFCPVCYGTDMICYGHNPQGSQRIQCRNCKK
                     VWTPKQYQKEITHPQAIETVQLFIPFQGASAVQKLYVLVSLDATRGNILHLSTNYTQH
                     QTGDSLRYSYKGNTEPTMHHRDIVQKVDMREAQFLRRSQFDEIQYGSAVLKRNGKGAI
                     LRPVITAHGHFRILKIRYPDVKTHIISHECFLRGAIITAWADQFRQQQGELWFVEEEI
                     SDSNADTPWHFKGTTYHGWWQNQWQRWEQGNNCKMVCLLTGACLERGANVSLATSRCF
                     ITWLTDQHDFTQSALLSAGRVTQMLTSLALKYNESLTPSC"
     gene            1384..2937
                     /locus_tag="ACHKBL_001382"
     CDS             1384..2937
                     /locus_tag="ACHKBL_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000694068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HFO5818273.1"
                     /translation="MKIKILAAGIALTLPFWACAKDVTIIYTNDLHAHVEPYKVPWIA
                     DGKRDIGGWANITTLVKQEKAKNKATWFFDAGDYFTGPYISSLTKGKAIIDIMNTMPF
                     DAVTIGNHEFDHGWDNTLLQLSQAKFPIVQGNVFYQNSSKSFWDKPYTIIEKDGVKIG
                     VIGLHGVFAFNDTVSAATRVGIEARDEIKWLQRYIDELKGKVDLTVALIHEGVPARQS
                     SMGGTDVRRALDKDIQTASQVKGLDILITGHAHVGTPEPIKVGNTLILSTDSGGIDVG
                     KLVLDYKEKPHNFTVKNFELKTIYADEWKPDPQTKQVIDGWNKKLDEVVQQTVAQSPV
                     ELKRAYGESASLGNLAADALLVAAGKNTQLALTNSGGIRNEIPAGAITMGGVISTFPF
                     PNELVTMELTGKQLRSLMEHGASLSNGVLQVSKGLEMKYDSSKPVGQRVITLTLNGKP
                     IEDATVYHIATQSFLADGGDGFTAFTEGKARNTTGGYYVYHAVVDYFKAGNTITDEQL
                     NGMRVKDIK"
     gene            3319..4209
                     /gene="metF"
                     /locus_tag="ACHKBL_001383"
     CDS             3319..4209
                     /gene="metF"
                     /locus_tag="ACHKBL_001383"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HFO5818274.1"
                     /translation="MSFFHASQRDALNQSLAEVQGQINVSFEFFPPRTSEMEQTLWNS
                     IDRLSSLKPKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPAELRTI
                     ARDYWNNGIRHIVALRGDLPPGSGKPEMYASDLVTLLKEVADFDISVAAYPEVHPEAK
                     SAQADLLNLRRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFK
                     QAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYT
                     LNRAEMSYAICHTLGVRPGL"
     gene            4538..6718
                     /gene="katG"
                     /locus_tag="ACHKBL_001384"
     CDS             4538..6718
                     /gene="katG"
                     /locus_tag="ACHKBL_001384"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HFO5818275.1"
                     /translation="MSTSDDIHNTTATGKCPFHQGGHDQSAGGGTTTRDWWPNQLRVD
                     LLNQHSNRSNPLGEDFDYRKEFSKLDYYGLKKDLKALLTESQPWWPADWGSYAGLFIR
                     MAWHGAGTYRSIDGRGGAGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGQKISWA
                     DLFILAGNVALENSGFRTFGFGAGREDVWEPDLDVNWGDEKAWLTHRHPEALAKAPLG
                     ATEMGLIYVNPEGPDHSGEPLSAAAAIRATFGNMGMNDEETVALIAGGHTLGKTHGAG
                     PTSNVGPDPEAAPIEEQGLGWASTYGSGVGADAITSGLEVVWTQTPTQWSNYFFENLF
                     KYEWVQTRSPAGAIQFEAVDAPEIIPDPFDPSKKRKPTMLVTDLTLRFDPEFEKISRR
                     FLNDPQAFNEAFARAWFKLTHRDMGPKSRYIGPEVPKEDLIWQDPLPQPIYNPTEQDI
                     IDLKFAIADSGLSVSELVSVAWASASTFRGGDKRGGANGARLALMPQRDWDVNAAAVR
                     ALPVLEKIQKESGKASLADIIVLAGVVGVEKAASAAGLSIHVPFAPGRVDARQDQTDI
                     EMFELLEPIADGFRNYRARLDVSTTESLLIDKAQQLTLTAPEMTALVGGMRVLGANFD
                     GSKNGVFTDRVGVLSNDFFVNLLDMRYEWKATDESKELFEGRDRETGEVKYTASRADL
                     VFGSNSVLRAVAEVYASSDAHEKFVKDFVAAWVKVMNLDRFDLL"
     gene            6812..7717
                     /gene="yijE"
                     /locus_tag="ACHKBL_001385"
     CDS             6812..7717
                     /gene="yijE"
                     /locus_tag="ACHKBL_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine transporter YijE"
                     /protein_id="HFO5818276.1"
                     /translation="MSAAGKSNPLAISGLVVLTLIWSYSWIFMKQVTSYIGAFDFTAL
                     RCIFGALVLFIVLLLRGRGMRPTPFKYTLAIALLQTCGMVGLAQWALVSGGAGKVAIL
                     SYTMPFWVVIFAALFLGERLRRGQYFAILIAAFGLFLVLQPWQLDFSSMKSAMLAILS
                     GVSWGASAIVAKRLYARHPRVDLLSLTSWQMLYAALVMSVVALLVPQREIDWQPTVFW
                     ALAYSAILATALAWSLWLFVLKNLPASIASLSTLAVPVCGVLFSWWLLGENPGAVEGS
                     GIVLIVLALALVSRKKKEAVSVKRI"
     gene            complement(7744..8361)
                     /locus_tag="ACHKBL_001386"
     CDS             complement(7744..8361)
                     /locus_tag="ACHKBL_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
                     /protein_id="HFO5818277.1"
                     /translation="MKASLALFSLLTVFTSYSLKSPAVPPTVVQIQANTNLAIADGAR
                     QQIGSTLFYDPAYVQLTYPGGDVPQERGVCSDVVIRALRSQKVDLQKLVHEDMAKNFA
                     AYPQKWQLKRPDSNIDHRRVPNLETWFTRHDKTRPTSKNPSDYQAGDIVSWRLDNGLA
                     HIGVVSDGFARDGTPLVIHNIGAGAQEEDVLFSWRMVGHYRYFAK"
     gene            complement(8637..9740)
                     /gene="gldA"
                     /locus_tag="ACHKBL_001387"
     CDS             complement(8637..9740)
                     /gene="gldA"
                     /locus_tag="ACHKBL_001387"
                     /EC_number="1.1.1.6"
                     /EC_number="1.1.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional L-1,2-propanediol
                     dehydrogenase/glycerol dehydrogenase"
                     /protein_id="HFO5818278.1"
                     /translation="MDRIIQSPGKYIQGADVINRLGEYLKPLAERWLVVGDKFVLGFA
                     QSTVEKSFKDAGLVVEIAPFGGECSQNEIDRLRGIAETAQCGAILGIGGGKTLDTAKA
                     LAHFMGVPVAIAPTIASTDAPCSALSVIYTDDGEFDRYLMLPNNPNMVIVDTKIVAGA
                     PARLLAAGIGDALATWFEARACSRSGATTMAGGKCTQAALALAELCYNTLLEEGEKAM
                     LAAEQHVVTPALERVIEANTYLSGVGFESGGLAAAHAVHNGLTAIPDAHHYYHGEKVA
                     FGTLTQLVLENAPVEEIETVAALSHAVGLPITLAQLDIKEDVPAKMRIVAEAACAEGE
                     TIHNMPGGATPDQVYAALLVADQYGQRFLQEWE"
     gene            complement(9751..10413)
                     /gene="fsa"
                     /locus_tag="ACHKBL_001388"
     CDS             complement(9751..10413)
                     /gene="fsa"
                     /locus_tag="ACHKBL_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HFO5818279.1"
                     /translation="MELYLDTANVAEVERLARIFPIAGVTTNPSIIAASKESIWEVLP
                     RLQKAIGDEGILFAQTMSRDAQGMVKEAKRLRDAIPGIVVKIPVTSEGLAAIKMLKKE
                     GITTLGTAVYSAAQGLLAALAGAKYVAPYVNRVDAQGGDGIRTVQELQALLEMHAPES
                     MVLAASFKTPRQALDCLLAGCESITLPLDVAQQMLNTPAVESAIEKFEHDWNAAFGTT
                     HL"
     gene            complement(10425..12926)
                     /gene="ptsP"
                     /locus_tag="ACHKBL_001389"
     CDS             complement(10425..12926)
                     /gene="ptsP"
                     /locus_tag="ACHKBL_001389"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFO5818280.1"
                     /translation="MALIVEFICELPNGVHARPASHVETLCNTFSSQIEWHNLRTDRK
                     GNAKSALALIGTDTLVGDNCQLLISGADEQEAHQRLSQWLRDEFPHCDAPLAEVKSDE
                     LEPLPVSLTNLNPQIIRARTVCSGSAGGILTPISSLDLNALGNLPAAKGVDAEQSALE
                     NGLTLVMKNIEFRLLDSDGATSAILEAHRSLAGDTSLREHLLAGVSAGLSCAEAIVAS
                     ANHFCEEFARSSSSYLQERALDVRDVCFQLLQQIYGEQRFPAPGKLTQPAICMADELT
                     PSQFLELDKNHLKGLLLKSGGTTSHTVILARSFNIPTLVGVDIDALTPWQHQTIYIDG
                     NAGAIVVEPGEAVARYYQQEARVQDALREQQRVWLTQQARTADGIRIEIAANIAHSVE
                     AQAAFGNGAEGVGLFRTEMLYMDRTSAPGESELYNIFCQALESANGRSIIVRTMDIGG
                     DKPVDYLNIPAEANPFLGYRAVRIYEEYASLFTTQLRSILRASAHGSLKIMIPMISSM
                     EEILWVKEKLAEAKQQLRNEHIPFDEKIPLGIMLEVPSVMFIIDQCCEEIDFFSIGSN
                     DLTQYLLAVDRDNAKVTRHYNSLNPAFLRALDYAVQAVHRQGKWIGLCGELGAKGSVL
                     PLLVGLGLDELSMSAPSIPAAKARMAQLDSRECRKLLNQAMACRTSLEVEHLLAQFRM
                     TQQDAPLVTAECITLESDWRSKEEVLKGMTDNLLLAGRCRYPRKLEADLWAREAVFST
                     GLGFSFAIPHSKSEHIEQSTISVARLAAPVRWGDDEAQFIIMLTLNKHAAGDQHMRIF
                     SRLARRIMHEEFRNALVNAASADAIASLLQHELEL"
     gene            13235..14314
                     /locus_tag="ACHKBL_001390"
     CDS             13235..14314
                     /locus_tag="ACHKBL_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HFO5818281.1"
                     /translation="MNELVQILKNTRQHLMTGVSHMIPFVVSGGILLAVSVMLYGKGA
                     VPDAVADPNLKKLFDIGVAGLTLMVPFLAAYIGYSIAERSALAPCAIGAWVGNSFGAG
                     FFGALIAGIIGGIVVHYLKKIPVHKVLRSVMPIFIIPIVGTLITAGIMMWGLGEPVGA
                     LTNSLTQWLQGMQQGSIVMLAVIMGLMLAFDMGGPVNKVAYAFMLICVAQGVYTVVAI
                     AAVGICIPPLGMGLATLIGRKNFSAEERETGKAALVMGCVGVTEGAIPFAAADPLRVI
                     PSIMVGSVCGAVTAALVGAQCYAGWGGLIVLPVVEGKLGYIAAVAVGAVVTAVCVNVL
                     KSLARKNGSSTDEKEDDLDLDFEIN"
     gene            14329..14649
                     /locus_tag="ACHKBL_001391"
     CDS             14329..14649
                     /locus_tag="ACHKBL_001391"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HFO5818282.1"
                     /translation="MTKIIAVTACPSGVAHTYMAAEALESAAKAKGWEVKVETQGSIG
                     LENELTAEDVASADMVILTKDIGIKFEERFAGKTIVRVNISDAVKRADAIMSKIEAHL
                     AQTA"
     gene            14700..16997
                     /locus_tag="ACHKBL_001392"
     CDS             14700..16997
                     /locus_tag="ACHKBL_001392"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFO5818283.1"
                     /translation="MTNRISRLKTALFANTREISLERALLYTASHRQTEGEPVILRRA
                     KATAYILEHVEISIRDEELIAGNRTVKPRAGIMSPEMDPYWLLKELDQFPTRPQDRFA
                     ISEEDKRIYREELFPYWEKRSMKDFINGQMTDEVKAATSTQIFSINQTDKGQGHIIID
                     YPRLLNHGLGELVAQMQQHCQQQPENHFYQAALLLLEASQKHILRYAELAETMAASCT
                     DAQRREELLTIAEISRHNAEHKPQTFWQACQLFWYMNIILQYESNASSLSLGRFDQYM
                     LPFYQASLTQGEDPAFLKELLESLWVKCNDIVLLRSTSSARYFAGFPTGYTALLGGLT
                     ENGRSAVNVLSFLCLDAYQSVQLPQPNLGVRTNALIDTPFLMKTAETIRLGTGIPQIF
                     NDEVVVPAFLNRGVSLEDARDYSVVGCVELSIPGRTYGLHDIAMFNLLKVMEICLHEN
                     EGNAALTYEGLLEQIRAKISHYITLMVEGSNICDIGHRDWAPVPLLSSFISDCLEKGS
                     DITDGGARYNFSGVQGIGIANLSDSLHALKGMVFEQQRLSFDELLSVLKANFATPEGE
                     KVRARLINRFEKYGNDIDEVDNISAELLRHYCKEVEKYQNPRSGYFTPGSYTVSAHVP
                     LGSVVGATPDGRFAGEQLADGGLSPMLGQDAQGPTAVLKSVSKLDNTLLSNGTLLNVK
                     FTPATLEGEAGLRKLADFLRAFTQLKLQHIQFNVVNADTLREAQQRPQDYAGLVVRVA
                     GYSAFFVELSKEIQDDIIRRTAHQL"
     gene            16963..17841
                     /locus_tag="ACHKBL_001393"
     CDS             16963..17841
                     /locus_tag="ACHKBL_001393"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418387.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[formate-C-acetyltransferase]-activating enzyme"
                     /protein_id="HFO5818284.1"
                     /translation="MTSSAGQRISCNVVETRRNDVARIFNIQRYSLNDGEGIRTVVFF
                     KGCPHLCPWCANPESISGKIQTVRREAKCLHCAKCLRDADECPSGAFERIGRDISLDA
                     LEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETAGDAPAS
                     KLLPLAKLCDEVLFDLKIMDATQARDVVKMNLPRVLENLRLLVSEGVNVIPRLPLIPG
                     FTLSQENMQQALDVLIPLNIRQIHLLPFHQYGEPKYRLLGKTWSMKEVPAPSSADVAT
                     MREMAERAGFQVTVGG"
     gene            17843..18184
                     /locus_tag="ACHKBL_001394"
     CDS             17843..18184
                     /locus_tag="ACHKBL_001394"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HFO5818285.1"
                     /translation="MAYLVAVTACVSGVAHTYMAAERLEKLCLLEKWGVSIETQGALG
                     TENRLADEDIRRADVALLITDIELAGAERFDHCRYVQCSIYAFLREPQRVMSAVRKVL
                     SAPQQTHLILE"
     gene            complement(18171..19022)
                     /locus_tag="ACHKBL_001395"
     CDS             complement(18171..19022)
                     /locus_tag="ACHKBL_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFO5818286.1"
                     /translation="MYHDVSYLLSRLINGPLSLRQIYFASSNGPVPDLAYQVDFPRLE
                     IVLEGEFIDTGAGAALVPGDVLYVPAGGWNFPQWQAPATTFSVLFGKQQLGFSVVQWD
                     GKQYQNLAKQHVARRGPRIGSFLLQTLNEMQMQSQEQQTARLIVASLLSHCRDLLGSQ
                     IQTASRSQALFEAIRDYIDERYASALTRESVAQAFYISPNYLSHLFQKTGATGFNEYL
                     NHTRLEHAKTLLKGYDLKVKEVAHACGFVDSNYFCRLFRKNTERSPSEYRRQYHSQLT
                     EKPTTPE"
     gene            complement(19248..20981)
                     /locus_tag="ACHKBL_001396"
     CDS             complement(19248..20981)
                     /locus_tag="ACHKBL_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HFO5818287.1"
                     /translation="MHSTEVQAKPLFSWKALGWALLYFWFFSTLLQAIIYISGYSGTN
                     GIRDSLLFSSLWLIPVFLFPKRIKIIAAVIGVVLWAASLAALCYYVIYGQEFSQSVLF
                     VMFETNTNEASEYLSQYFSLKIVLIALAYTAVAVLLWTRLRPVYIPKPWRYVVSFALL
                     YGLILHPIAMNTFIKNKPFEKTLDNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNE
                     NNALPPLANFKDESGNEPRTLVLVIGESTQRGRMSLYGYPRETTPELDALHKTDPNLT
                     VFNNVVTSRPYTIEILQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTMT
                     ARNTMLTVFSRQTDKQYYMNQQRTQSAREYDTNVLKPFQEVLKDPAPKKLIIVHLLGT
                     HIKYKYRYPEDQGKFDGNTEHVPPGLNAEELESYNDYDNANLYNDHVVASLIKDFKAT
                     DPNGFLVYFSDHGEEVYDTPPHKTQGRNEDNPTRHMYTIPFLLWTSEKWQATHPRDFS
                     QDVDRKYSLAELIHTWSDLAGLSYDGYDPTRSVVNPQFKETTRWIGNPYKKNALIDYD
                     TLPYGDQVGNQ"
     gene            complement(21164..23815)
                     /gene="ppc"
                     /locus_tag="ACHKBL_001397"
     CDS             complement(21164..23815)
                     /gene="ppc"
                     /locus_tag="ACHKBL_001397"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004203687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HFO5818288.1"
                     /translation="MNEQYSALRSNVSMLGKVLGETIKDALGEHILERVETIRKLSKS
                     SRAGNDANRQELLTTLQNLSNDELLPVARAFSQFLNLANTAEQYHSISPKGEAASNPE
                     VIARTLRKLKNQPELSEDTIKKAVESLSLELVLTAHPTEITRRTLIHKMVEVNACLKQ
                     LDNKDIADYEHNQLMRRLRQLIAQSWHTDEIRKLRPSPVDEAKWGFAVVENSLWQGVP
                     NYLRELNEQLEENLGYKLPVEFVPVRFTSWMGGDRDGNPNVTADITRHVLLLSRWKAT
                     DLFLKDIQVLVSELSMVEATPELLALVGEEGAAEPYRYLMKNLRSRLMATQAWLEARL
                     KGEELPKPEGLLTQNEELWEPLYACYQSLQACGMGIIANGDLLDTLRRVKCFGVPLVR
                     IDIRQESTRHTEALGELTRYLGIGDYESWSEADKQAFLIRELNSKRPLLPRNWQPSAE
                     TREVLDTCQVIAEAPQGSIAAYVISMAKTPSDVLAVHLLLKEAGIGFAMPVAPLFETL
                     DDLNNANDVMTQLLNIDWYRGLIQGKQMVMIGYSDSAKDAGVMAASWAQYQAQDALIK
                     TCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLP
                     EITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDVYRGYVRENKDFVPYF
                     RSATPEQELGKLPLGSRPAKRRPTGGVESLRAIPWIFAWTQNRLMLPAWLGAGTALQK
                     VVEDGKQSELEAMCRDWPFFSTRLGMLEMVFAKADLWLAEYYDQRLVDKALWPLGKEL
                     RNLQEEDIKVVLAIANDSHLMADLPWIAESIQLRNIYTDPLNVLQAELLHRSRQAEKE
                     GHEPDPRVEQALMVTIAGIAAGMRNTG"
     gene            complement(24117..25268)
                     /gene="argE"
                     /locus_tag="ACHKBL_001398"
     CDS             complement(24117..25268)
                     /gene="argE"
                     /locus_tag="ACHKBL_001398"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HFO5818289.1"
                     /translation="MKNKLPPFIEIYRALIATPSISATEEALDQSNADLITLLADWFK
                     DLGFNVEVQPVPGTRNKFNMLASCGQGAGGLLLAGHTDTVPFDDGRWTRDPFTLTEHD
                     GKLYGLGTADMKGFFAFILDALRDVDVTKLAKPLYILATADEETSMAGARYFAETTAL
                     RPDCAIIGEPTSLQPVRAHKGHISNAIRIQGQSGHSSDPARGVNAIELMHDAIGHILQ
                     LRDNLKERYHYDAFTVPYPTLNLGHIHGGDASNRICACCELHMDIRPLPGMTLNELNG
                     LLNDALAPVSERWPGRLTVDELHPPIPGYECPPNHKLVEVVEKLLGAKTEVVNYCTEA
                     PFIQTLCPTLVLGPGSINQAHQPDEYLETRFIKPTRELITQVIHHFCWH"
     gene            25422..26426
                     /gene="argC"
                     /locus_tag="ACHKBL_001399"
     CDS             25422..26426
                     /gene="argC"
                     /locus_tag="ACHKBL_001399"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0070401 - NADP+ binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HFO5818290.1"
                     /translation="MLNTLIVGASGYAGAELVTYVNRHPHMNITALTVSAQSNDAGKL
                     ISDLHPQLKGIVDLPLQPMSDISEFSPGVDVVFLATAHEVSHDLAPQFLEAGCVVFDL
                     SGAFRVNDVAFYEKYYGFTHQYPELLEQAAYGLAEWCGNKLKEANLIAVPGCYPTAAQ
                     LALKPLIDADLLDLNQWPVINATSGVSGAGRKAAISNSFCEVSLQPYGVFTHRHQPEI
                     ATHLGADVIFTPHLGNFPRGILETITCRLKPGVSQVQVAQALQQAYAHKPLVRLYDKG
                     VPALKNVVGLPFCDIGFAVQGEHLIIVATEDNLLKGAAAQAVQCANIRFGYAETQSLI
                     "
     gene            26434..27210
                     /gene="argB"
                     /locus_tag="ACHKBL_001400"
     CDS             26434..27210
                     /gene="argB"
                     /locus_tag="ACHKBL_001400"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HFO5818291.1"
                     /translation="MMNPLIIKLGGVLLDSEEALERLFSALVNYRESHQRPLVIVHGG
                     GCVVDELMKGLNLPVKKKNGLRVTPADQIDIITGALAGTANKTLLAWAKKHQIAAVGL
                     FLGDGDSVKVTQLDEELGHVGLAQPGSPKLINSLLENGYLPVVSSIGVTDEGQLMNVN
                     ADQAATALAATLGADLILLSDVSGILDGKGQRIAEMTAAKAEQLIEQGIITDGMIVKV
                     NAALDAARTLGRPVDIASWRHAEQLPALFNGMPMGTRILA"
     gene            27271..28644
                     /gene="argH"
                     /locus_tag="ACHKBL_001401"
     CDS             27271..28644
                     /gene="argH"
                     /locus_tag="ACHKBL_001401"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HFO5818292.1"
                     /translation="MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKAL
                     VTVGVLTAEEQAQLEEALNVLLEDVRARPQQILESDAEDIHSWVESKLIDKVGQLGKK
                     LHTGRSRNDQVATDLKLWCKDTVSELLTANRQLQSALVETAQNNQDAVMPGYTHLQRA
                     QPVTFAHWCLAYVEMLARDESRLQDALKRLDVSPLGCGALAGTAYEIDREQLAGWLGF
                     ASATRNSLDSVSDRDHVLELLSAAAIGMVHLSRFAEDLIFFNTGEAGFVELSDRVTSG
                     SSLMPQKKNPDALELIRGKCGRVQGALTGMMMTLKGLPLAYNKDMQEDKEGLFDALDT
                     WLDCLHMAALVLDGIQVKRPRCQEAAQQGYANATELADYLVAKGVPFREAHHIVGEAV
                     VEAIRQGKALEDLPLSELQKFSQVIGEDVYPILSLQSCLDKRAAKGGVSPQQVAQAIA
                     FAQARLE"
BASE COUNT         6692 a         7236 c         7899 g         7019 t
ORIGIN      
        1 agtaacaaat aatgttatta attaaacatt gaccatgatt atatttcacg gtcatacatt
       61 tatatattta attaaatgaa aggtagtgtt ctctcttaat ttatggtcgt ttgtcgagtg
      121 tattgactaa caagatggag tgagcgattc attgtatttt aacgccagtg aggtcagcat
      181 ttgtgttacg cgacctgcgg ataataacgc gctttgggta aagtcgtgtt ggtctgtcag
      241 ccatgtgata aaacaacgac tggttgccag agaaacattt gcgccccttt ctaagcaggc
      301 tcccgtgagt aggcagacca tcttgcagtt attcccctgc tcccagcgct gccactgatt
      361 ttgccaccaa ccatggtatg tcgttccctt aaaatgccag ggcgtatcag cgttactgtc
      421 actgatttct tcttctacga accaaagttc accttgttgt tggcggaact gatctgccca
      481 ggctgtaata attgcgcctc ttagaaaaca ttcgtgtgaa ataatatgcg ttttgacatc
      541 tggatagcgg attttcagta ttctgaaatg cccgtgtgcc gtgataaccg ggcgtagtat
      601 ggctccctta ccgttgcgct tgagtacagc actgccatac tgaatttcat cgaactggct
      661 ccggcgcaag aattgcgctt cacgcatatc taccttctgc acgatatcgc gatggtgcat
      721 agttggttct gtgttgccct tgtaactgta ccgcagactg tctcctgtct gatgttgcgt
      781 ataattcgtg gagagatgaa gaatgttgcc acgagtggcg tcaagactga ctaaaacata
      841 cagtttttgt actgcgctgg ccccttgaaa gggtatgaac agttgaacgg tttcaatggc
      901 ctggggatgc gtaatttctt tctgatactg tttaggcgtc cagacttttt tgcagttacg
      961 gcattgaatg cgttgcgaac cctgtggatt gtgaccataa caaatcatgt ctgtgccata
     1021 acataccggg cagaaatgac ccttctccga ggcccacgta gataagtgaa ctgacaacca
     1081 gtcacgaaat tgttgctcgt caaacaacgg cgggtaactg ccacatgccc gacagtgcag
     1141 tgccggataa cccagtttat agtctggcca actgtaatct tccgatgagg ccaatcccaa
     1201 atttcggcaa ccgaacgttt tacaggcatt aatcgtaaac agagtcgaca tatatcacca
     1261 acgatttcac aaggtcgttg attttgtccg gcaactatcc cctgcagatg tgattactct
     1321 cacataaata agtctttcag aacgtaatgt agcaatcctt tatctcactg aaagggattg
     1381 tttatgaaaa ttaaaatact tgctgcgggt atcgcattaa cactgccgtt ctgggcttgc
     1441 gctaaagatg tcactattat ttataccaac gacctccatg cgcatgtaga accttataaa
     1501 gtgccgtgga tcgctgacgg taaacgggat attggcggct gggcaaatat cactacgctg
     1561 gttaagcaag aaaaagcaaa aaataaagcg acatggtttt ttgatgcggg cgactatttt
     1621 accgggccat atatcagcag cctgaccaaa ggtaaggcta ttatcgatat tatgaatact
     1681 atgccgtttg atgccgtcac gataggcaac catgaatttg accacggctg ggacaacaca
     1741 ttattacagt tgagtcaggc aaaattcccg attgtgcagg gcaatgtttt ttatcagaac
     1801 agcagtaaat cattctggga taagccctat accatcatcg aaaaagacgg tgtgaaaata
     1861 ggcgttatag gtttgcacgg tgtatttgcc tttaatgaca cagtatctgc tgccacgcga
     1921 gtgggtattg aggcgcgtga tgaaattaaa tggctacaac gttatatcga tgaacttaaa
     1981 ggcaaggttg atttgaccgt cgccctgatc catgaaggtg ttccggcccg ccagtccagt
     2041 atgggaggca cagatgtgcg tcgcgcgctg gataaagata ttcagacggc aagtcaggtg
     2101 aaagggctgg atattttgat caccggacat gcacatgtgg gtacgccgga accgattaaa
     2161 gtcggcaata cgctcatcct ctccactgac agcggcggga ttgatgtggg caaactggtt
     2221 ctcgactaca aagagaagcc gcataatttt acggtgaaaa acttcgagct taaaaccatt
     2281 tacgctgacg agtggaagcc cgatccacaa acgaaacagg tgatcgacgg ctggaataaa
     2341 aagcttgatg aagtggtgca acaaacggtg gcgcaatcgc cggttgaatt aaaacgtgcc
     2401 tatggtgaat cggcttccct cgggaacctg gcggctgacg ctttactggt agcggcgggt
     2461 aaaaacaccc aattggcgtt aaccaactct ggcggcattc gcaatgagat ccccgcaggg
     2521 gcgatcacga tgggcggcgt catcagtacg ttcccgttcc ccaacgaact ggtgacgatg
     2581 gaactcacgg gtaaacaatt acgcagtctg atggaacacg gcgcaagttt aagcaatggc
     2641 gttttacagg tatcgaaagg cctggaaatg aagtacgaca gcagcaagcc ggttggtcag
     2701 cgggtaatca cactgactct gaatggcaaa cccattgaag atgcgacggt ttatcacatt
     2761 gctactcaga gtttccttgc tgatggtgga gatggtttta ccgcctttac cgaagggaaa
     2821 gcgcgtaaca caacgggcgg ttattacgtt tatcacgcgg tggttgatta cttcaaagcg
     2881 ggcaacacca ttacggatga acagctcaac ggtatgcgcg tgaaggatat caaataaaag
     2941 tgcgcactga attgactcaa ctgtttgtta cccgttgatt gcgcagatcc tgtattcggc
     3001 ctacgcgagg gcgcaggccg aatcagatgc cttagcatca ccatccggtg gcaggacgtt
     3061 gtttttcgaa ttagcccagg tgtcgctgtg ttgtcagaat atgagtcaaa acgtgcgagg
     3121 tggattgtgc tatagagaaa tcttttatcg cctctaatta agatgagttt ttttcatctt
     3181 ccctgatttt tcctcaccat cattggttat ttttcggttg acgcccttcg gcttttcctt
     3241 catctttaca tctggacgtc taaacggata gatgtgcaca acacaacata taacgataag
     3301 cgattgatga ggtaaggtat gagctttttt cacgccagcc agcgggatgc cctgaatcag
     3361 agtctggcag aagtccaggg gcagattaac gtttcgttcg aatttttccc gccgcgtacc
     3421 agtgaaatgg agcagaccct gtggaactcc atcgatcgcc ttagcagcct gaaaccgaag
     3481 tttgtatcgg tgacctatgg cgcaaactcc ggcgagcgcg accgtacgca cagcattatt
     3541 aaaggcatta aagatcgcac cggcctggaa gcggcaccgc atcttacctg tattgatgcg
     3601 acgcctgccg agctgcggac cattgcacgc gactactgga ataacggtat tcgccatatc
     3661 gtggcgctgc gtggcgacct gccgccggga agtggtaagc cagaaatgta tgcttctgac
     3721 ctggtgacac tgttaaaaga agtggcagat ttcgatatct ccgtggcggc gtatccggaa
     3781 gtacatccag aagcgaaaag tgcccaggcc gatttactca acctgagacg aaaagtggat
     3841 gccggagcca accgcgccat tactcagttc ttcttcgatg tcgaaagcta cctgcgtttt
     3901 cgcgatcgct gcgtctcggc aggcattgat gtggaaatca ttccgggaat tttgccggtt
     3961 tcgaacttta aacaggcgaa gaaatttgcc gacatgacca acgtgcgtat tccggcgtgg
     4021 atggcacaaa tgttcgacgg cctggatgac gatgctgaaa cccgcaagct ggtgggcgcg
     4081 aatattgcca tggatatggt gaagatttta agccgtgaag gagtgaaaga tttccacttc
     4141 tatacgctta accgcgctga aatgagttac gcgatttgcc atacactggg tgttcgacct
     4201 ggtttataaa tagtgtgcct tttgtgaaaa ttacacagtg atcacaaatt ttaaacagag
     4261 cacaaaatgc tgcctcgaaa tgagggcggg aaaataaggt tatcagcctt gttttctccc
     4321 tcattacttg aaggatacaa atctaaaacc cttttttata aagcatttgt cctaaatcgg
     4381 acataatcaa aaagtcttaa ttacgatcaa tttgatctac atctctttaa ccaacaatat
     4441 gtaagatctc aactatcgca tccgtggatt aattcaatta taacttctct ctaacgctgt
     4501 gtatcgtaac ggtaacactg tagaggggag cacattgatg agcacgtcag acgatatcca
     4561 taacacgaca gccaccggca aatgcccgtt ccatcagggc ggtcacgacc agagcgcagg
     4621 tgggggcaca accacgcgcg actggtggcc aaatcaactc cgtgttgacc tgttaaacca
     4681 acattctaat cgttctaacc cgctgggtga ggactttgac taccgcaaag aattcagcaa
     4741 attagattac tacggactga aaaaagatct gaaagccctg ctgacagaat ctcaaccgtg
     4801 gtggcctgcc gactggggca gctacgccgg tctgtttatt cgtatggcct ggcacggtgc
     4861 gggtacttac cgttcaatcg atgggcgcgg cggcgcgggt cgtggtcagc aacgttttgc
     4921 accgctgaac tcctggccgg ataacgtaag cctcgataaa gcgcgccgcc tgttgtggcc
     4981 aatcaaacag aaatatggtc agaaaatctc ctgggccgac ctgtttatcc tcgcgggtaa
     5041 cgtggcgctg gaaaactccg gcttccgtac cttcggtttt ggtgccggtc gtgaagacgt
     5101 ctgggaaccg gatctggatg ttaactgggg tgatgaaaaa gcctggctga cccaccgtca
     5161 tccggaagca ctggcgaaag caccgctggg cgcaaccgag atgggcctga tttacgttaa
     5221 cccggaaggc ccggatcaca gcggcgaacc gctttctgcg gcagcagcta tccgcgcgac
     5281 cttcggcaac atgggcatga acgacgaaga aaccgtggcg ctgattgcgg gtggtcatac
     5341 gctgggtaaa actcacggtg ccggtccgac atcaaatgta ggtcctgatc cagaagctgc
     5401 accgattgaa gaacaaggtt taggctgggc gagcacttac ggcagcggcg ttggcgcaga
     5461 tgccattacc tctggtctgg aagtagtctg gacccagacg ccgacccaat ggagcaacta
     5521 tttcttcgag aacctgttca aatatgagtg ggtacaaact cgcagcccgg ctggcgcaat
     5581 ccagttcgaa gcggtagacg caccggaaat tatcccggat ccgtttgatc cgtcgaagaa
     5641 acgtaaaccg acgatgctgg tgaccgacct gacgctgcgt tttgatcctg agttcgagaa
     5701 gatctctcgt cgtttcctca acgatccgca ggcgttcaac gaagcctttg ctcgtgcttg
     5761 gttcaaactg acgcacaggg atatggggcc gaaatctcgt tacatcgggc cggaagtacc
     5821 gaaagaagat ctgatctggc aagatccgct gccgcagccg atctacaacc cgaccgagca
     5881 ggacattatc gatctgaaat tcgcgattgc ggattctggt ctgtctgtta gtgagctggt
     5941 atcggtggcc tgggcgtctg cttctacctt ccgtggtggc gacaaacgtg gtggtgccaa
     6001 cggtgcgcgt ctggcattaa tgccgcagcg tgactgggat gtgaacgccg cagccgttcg
     6061 tgctctgcca gttctggaga aaattcagaa agagtctggc aaagcttcgc tggcggatat
     6121 catcgtgctg gcaggtgtgg ttggcgttga gaaagccgca agcgccgcag gtttgagcat
     6181 tcatgtaccg tttgcgccgg gccgcgttga tgcgcgtcag gatcagactg acattgagat
     6241 gttcgaactg ctggagccaa ttgctgacgg tttccgtaac tatcgcgctc gtctggacgt
     6301 ttccaccacc gagtcactgt tgattgataa agcacagcaa ctgacgctga ccgcgccgga
     6361 aatgactgcg ctggtgggcg ggatgcgcgt actgggtgcc aacttcgatg gcagcaaaaa
     6421 cggcgtcttc actgaccgcg ttggcgtatt gagcaatgac ttcttcgtga acttgctgga
     6481 tatgcgttac gagtggaaag cgaccgacga atcgaaagag ctgttcgaag gccgtgaccg
     6541 cgaaactggc gaagtgaaat acacagccag ccgtgcggat ctggtctttg gttctaactc
     6601 tgtcctgcgt gcggtggcgg aagtctacgc cagcagcgat gcccacgaga agtttgttaa
     6661 agacttcgtg gcagcgtggg tgaaagtgat gaacctcgac cgtttcgacc tgctgtaatc
     6721 tgaccttctt cagcgactgc ctttcatgca gtcgctgaag tttctttacc ggcgtatagt
     6781 gtccacagga aaactacaca ctggatctct catgtctgcc gcaggaaaga gcaacccact
     6841 ggcaatcagt ggcctggttg tgctcacact tatctggagt tatagctgga ttttcatgaa
     6901 gcaagtcacc agctacatcg gtgccttcga ctttaccgcc ttacgctgca ttttcggcgc
     6961 tctcgtttta ttcatcgtcc ttttattacg tggtcgcgga atgcgcccga caccgtttaa
     7021 atacacctta gccattgccc tgttacaaac ctgcgggatg gtcggcctgg cgcagtgggc
     7081 gctggtcagc ggaggagcgg ggaaggtggc aatcctgagc tataccatgc cgttctgggt
     7141 ggtaattttc gccgcgttgt ttctcggtga acgcctgcga cgcggacaat atttcgcgat
     7201 tctgattgcc gctttcggct tatttttggt gttgcagccg tggcaactcg atttctcttc
     7261 gatgaaaagc gccatgctgg caatcctctc cggtgtgagt tggggggcga gcgcgattgt
     7321 tgctaaacgt ctgtatgccc gccatccgcg cgtggattta ttgtcgctaa catcctggca
     7381 gatgctgtat gcggcgctgg tgatgagcgt ggtcgcttta ctggtgccgc aacgtgaaat
     7441 tgactggcaa cccaccgtgt tctgggcgct ggcctacagt gcgattctgg cgacagcgct
     7501 ggcgtggagc ttatggttgt ttgtattgaa aaacttgcct gccagtattg ccagcttaag
     7561 cacactggcc gttcccgttt gcggcgtact cttttcctgg tggctgctcg gtgagaatcc
     7621 gggggcagtt gaaggtagcg gtattgtgct gattgtactg gcactggcgc tggtgagccg
     7681 taagaaaaaa gaagccgtca gtgtaaaaag gatctgaatt ttttcttcat gtggggagat
     7741 ctcttattta gcaaaatacc gatagtgccc caccatccgc cagctaaaca gtacatcttc
     7801 ttcctgcgcg cctgcgccaa tgttatgtat caccagcggc gtaccgtcgc gggcgaagcc
     7861 atctgacacc accccaatat gtgccagccc gttatctaat cgccaggaga caatatcgcc
     7921 cgcttgatag tcactggggt tcttgctggt ggggcgtgtt ttatcgtggc gggtaaacca
     7981 ggtttccaga ttaggcaccc ggcggtgatc gatgttgctg tccgggcgct ttaactgcca
     8041 tttttgcggg tacgcagcaa aattcttcgc catatcttcg tgcaccagtt tctgcaaatc
     8101 gaccttctgg ctgcgcaatg cacggatcac cacatcggaa catacgccgc gttcttgcgg
     8161 gacatcaccg ccaggatagg taagctgcac atacgccgga tcgtaaaata gcgtgctgcc
     8221 gatttgctgt ctggcgccgt ctgcgatggc aagattggtg ttggcctgga tttgtaccac
     8281 ggttggtgga acggcgggag attttaagga gtaactggta aataccgtca gaagactgaa
     8341 taacgccagt gaagctttca tctgtttcgt ccgtgaaaat aagagtcaca gattcgacct
     8401 tccgggctaa cgattgttct gtggagaaaa tgtccagaag cggataaaaa aacacccgaa
     8461 caaggtggaa gtttcagatt actggcacag cgtgccggat gcggcgtgaa cgccttatcc
     8521 ggcctacaaa agtacgtaaa tttactatat tgcagagatt gcgtaggcct gataagcgta
     8581 gcgcatcagg caattttgcg ttcaaactcc cggacaagcc gggagtttgg agtaggttat
     8641 tcccactctt gcaggaaacg ctgaccatac tggtcggcta ccagcagtgc ggcgtaaacc
     8701 tgatctggcg tcgcgccgcc aggcatgttg tggatggttt caccttctgc acatgccgct
     8761 tctgccacaa ttcgcatttt cgccgggaca tcttctttaa tatccagttg agcgagagtt
     8821 attggcaaac ctaccgcatg gctaagtgca gctacggttt cgatttcctc caccggcgcg
     8881 ttttccagaa ccagctgcgt cagcgtacca aatgccactt tttcaccgtg gtaatagtga
     8941 tgcgcgtccg ggatagcggt caggccgtta tgcaccgcgt gagccgccgc cagcccgccg
     9001 ctttcaaaac caacgccgct caaataggtg ttcgcttcaa tcacgcgctc cagcgccggg
     9061 gttactacat gctgctcggc ggcaagcatc gctttttcgc cttcttccag cagggtgttg
     9121 tagcacagtt cagccagtgc cagcgccgcc tgggtgcact tgccgcccgc catggtggtc
     9181 gcgccgctac gagagcaagc acgcgcttca aaccaggttg ccagcgcatc gccgataccc
     9241 gccgctaaca gacgtgcagg tgcgccagcg acgattttgg tgtcgacaat gaccatattc
     9301 gggttatttg gcaacatcag atagcggtca aattcaccat catcggtgta gataacagac
     9361 aatgcgctgc acggtgcgtc ggtagaggcg atagtcggtg cgatcgctac cggaacaccc
     9421 atgaaatgtg ccagtgcttt ggcagtatcg agagtttttc cgccaccgat accgagaatt
     9481 gcgccacact gcgcagtctc cgcgatgcca cgcagacggt cgatctcatt ttgcgaacat
     9541 tcaccgccaa acggcgcaat ttctactacc agtccagcat ctttaaagct tttctcgaca
     9601 gtggattgag caaaacctaa aacaaatttg tcacccacca ctaaccagcg ttctgccagc
     9661 ggcttcaggt attcgcccag acgattaatc acatcagcgc cctggatgta tttacccggt
     9721 gattgaataa tgcggtccat aattgctcct ttagagatga gtagtgccaa atgcggcatt
     9781 ccagtcgtgt tcgaacttct ctatagctga ctctaccgca ggggtgttga gcatttgttg
     9841 cgctacatct aagggcaggg tgatggattc acatcctgcc agtaaacagt ccagcgcctg
     9901 acgtggtgtt ttaaagctgg cagccagcac catgctttct ggcgcatgca tttccaacag
     9961 cgcttgcagc tcctgtacag tacgaatgcc gtcaccgccc tgggcatcta cgcggttaac
    10021 gtatggagca acgtatttgg ctccagccag cgccgccagt aatccttgcg ctgcgctgta
    10081 cactgcggtt cccagcgtag taatgccttc tttcttcagc attttaattg ctgccagacc
    10141 ttcagaggtt accggaattt taaccacaat gcccggaata gcgtcgcgca ggcgtttcgc
    10201 ttctttcacc ataccctgcg cgtcgcggct catggtctga gcaaacagaa ttccctcatc
    10261 accgatcgct ttttgaaggc gcggcagcac ttcccagatg gactccttgc tggcagcgat
    10321 aatgctcggg ttagttgtca ccccggcaat cgggaatatg cgtgccagac gttcgacttc
    10381 tgcgacgtta gcggtgtcca gatacagttc catgatgttt ccttttacag ttccagttca
    10441 tgttgcagca ggctggcgat agcgtcggca gaggcggcgt taaccagcgc gttacggaat
    10501 tcttcgtgca tgatgcgacg agcgaggcgc gagaaaatgc gcatgtgctg atcgcccgca
    10561 gcatgtttgt tcagggttaa cataatgatg aattgcgctt catcatcgcc ccagcgcacc
    10621 ggcgctgcca gacgcgccac gctgatggtg gattgctcaa tgtgttctga tttgctgtgg
    10681 ggaatggcaa aactaaagcc cagaccggta gagaaaacgg cctcgcgcgc ccacaagtcg
    10741 gcttccagtt tacgcggata gcgacagcgg cccgccagca gcaggttatc ggtcatgcct
    10801 ttgagcactt cttctttgct gcgccagtcg ctttccagtg tgatgcactc ggcggtgacc
    10861 agcggtgcgt cttgttgggt catgcggaat tgcgccagca ggtgttccac ttccagcgaa
    10921 gtacggcagg ccattgcctg gttgagcaac ttgcggcact cacggctatc aagttgcgcc
    10981 atccgcgctt tcgccgccgg aattgatggt gcgctcatgc tgagttcatc cagccctaaa
    11041 ccgaccagca acggcagcac ggaacctttc gctcccagtt caccgcacag accaatccat
    11101 ttgccctggc gatgcaccgc ctgcacggcg taatcgagcg cccgcaagaa tgctggattc
    11161 aggctgttgt agtgacgagt caccttagcg ttatcgcgat ccactgccag tagatattgc
    11221 gtcaggtcat tactaccaat actaaagaaa tcaatctctt cgcagcattg atcgatgatg
    11281 aacatcaccg acggcacttc cagcatgatg ccgagtggaa tcttctcatc aaacggaatg
    11341 tgttcgttac gcagttgctg tttggcttcc gccagttttt ctttcaccca taagatctct
    11401 tccattgagg agatcatcgg gatcatgatt ttcaggctgc cgtgagcaga ggcgcgcagg
    11461 atcgaccgta gttgtgtggt aaacaacgac gcgtattctt cataaatacg caccgcgcga
    11521 taaccgagga acgggtttgc ctctgcggga atattcagat aatcaacggg tttgtcaccg
    11581 ccaatatcca tagtgcgcac aataatgctg cgtccgttgg cggactccag cgcctgacaa
    11641 aaaatgttgt acagctcgct ttcgcccggt gcactggtgc gatccatata gagcatttca
    11701 gtgcggaata aaccaacgcc ttccgcacca ttgccgaatg cggcctgcgc ttccacggag
    11761 tgagcgatgt tagcggcaat ttcaatgcgg ataccgtcag cggtacgggc ttgttgggtc
    11821 agccagacac gctgttgctc acgcagggcg tcctgtacgc gggcttcttg ctgataataa
    11881 cgggccacgg cttcccctgg ctcaaccaca atcgcccctg cgttgccgtc gatataaatc
    11941 gtttgatgct gccacggagt aagggcatca atatccacac ctaccagcgt cggaatgttg
    12001 aacgaacggg caaggatcac cgtatgtgag gtggtgccgc cacttttcag taatagacct
    12061 ttgaggtgat ttttatccag ttcgaggaac tggctggggg tcagttcatc agccatacaa
    12121 atggcgggct gcgtcagttt gcccggtgcc gggaagcgtt gctcaccgta gatttgctgg
    12181 agtaactgga agcagacgtc gcgtacgtcc agggcacgtt cttgcaggta gctgctgctg
    12241 gaacgggcga actcttcgca aaagtgattc gcactggcaa caatcgcttc ggcgcagctt
    12301 aatccggcgc tgacacctgc cagtaaatgt tcgcgcaggg aagtatcgcc agccagggat
    12361 cggtgagctt ccagtatcgc gctggtagca ccgtcgctat ccagcagacg aaattcaatg
    12421 tttttcatca ccagtgtcag gccgttttcc agcgcggatt gctcggcgtc aacgcctttg
    12481 gcagcaggaa gattacccag cgcattgaga tctaaagaag agatcggcgt cagaatgccg
    12541 cctgcactac cactgcacac ggtgcgagca cggataattt gcggattcag attggtcagt
    12601 gaaaccggca gtggttccag ttcgtcagat tttacttccg ccagcggtgc gtcgcagtgg
    12661 gggaattcat cgcgcagcca ttggcttaaa cgctggtgcg cttcctgttc gtcggccccg
    12721 gaaatcagta actggcagtt atcgcccacc agcgtatcgg tgccaatcag cgcaagggcg
    12781 cttttggcgt tgcccttgcg gtcagtgcgc aggttatgcc actcaatttg tgatgaaaaa
    12841 gtattacaca gcgtttcaac gtggcttgcc ggacgtgcat gtacgccgtt aggtagctca
    12901 caaataaatt ccacaatcag ggccattgcc tctctcccgg tacggttttc tgtttacagg
    12961 ataagagcgc acggcaacgg cctgccatgt gacaaatctg ccaaaagctg gacaaatgta
    13021 atgtaaccgt caatttgcga cgcgtctcac aagacgccgt tttgcggcat gcttccggat
    13081 catcgcaagt tatgaggcgg atcgcatttt tgtactgata ttacaaaagt ccagtaaatg
    13141 gcctttttat ccactgtttg ctccgcctgc gattgcctat tgtttcctcc atcaaaaata
    13201 tggacatggg cttttgcaag cccgggagca gcttatgaat gagttggtgc agatcctgaa
    13261 aaatacccgt cagcatttaa tgacgggcgt ttcacacatg attcccttcg tggtatcggg
    13321 cggtattttg ctggcggttt ccgtcatgtt gtatggcaaa ggcgcagtgc cggatgccgt
    13381 agccgatccg aatctgaaaa aactgtttga tatcggcgtt gcgggtttga cgctgatggt
    13441 gcctttcctc gccgcttaca ttggttactc cattgcagag cgttctgcgc tggctccgtg
    13501 cgctatcggt gcctgggttg gtaacagctt tggtgcgggc ttctttggtg cactgatcgc
    13561 cgggattatc ggcggcatcg tggtgcatta cctgaagaaa attccggtgc ataaagttct
    13621 gcgctcggtg atgccaatct tcatcattcc gatcgtcggc acactgatta ccgcaggcat
    13681 catgatgtgg gggctgggcg agcctgtggg ggcattgacc aacagcctga ctcagtggct
    13741 tcaggggatg cagcagggca gcattgttat gctggcggtg atcatgggcc tgatgctggc
    13801 gttcgatatg ggcggtccgg ttaacaaagt ggcctatgcc ttcatgctga tttgcgttgc
    13861 tcagggtgtt tataccgtgg tggccattgc tgccgtgggt atttgtattc cgccgctggg
    13921 gatggggctg gcgacgctga ttggtcgtaa aaatttctcc gcagaagagc gcgaaaccgg
    13981 taaagcggcg ctggtgatgg ggtgcgttgg ggttactgaa ggggcgattc ctttcgccgc
    14041 tgccgatccg ctgcgtgtta ttccttccat catggtcggt tcagtttgtg gtgcagtaac
    14101 tgcggcgctg gtcggtgcgc agtgctatgc aggctggggc ggtctgattg tgctgccggt
    14161 ggttgaaggc aagctgggtt atatcgcagc agtagctgtc ggagcagtgg tgacggctgt
    14221 ttgtgtgaac gtgctgaaaa gtctggcgcg taaaaatggg tcttcgactg atgaaaaaga
    14281 agacgacctg gatttggatt ttgaaattaa ttaattgagg aacgagccat gacgaaaatt
    14341 attgcagtaa ccgcgtgtcc atctggtgtt gcccatacct atatggcggc agaggcgctg
    14401 gaaagtgccg cgaaagcgaa aggctgggaa gtgaaagtag aaacccaggg ctcgattggt
    14461 ctggaaaacg aactgactgc ggaagacgtg gcgagcgccg atatggtgat cctgaccaaa
    14521 gatatcggca tcaagtttga agagcgtttt gctggcaaga ccatcgtgcg cgtcaacatc
    14581 agcgacgcgg taaaacgcgc tgatgccatt atgagcaaaa ttgaagctca cctggcacaa
    14641 accgcttaag ttcttatccc gcccgtaagg gcgggttcgc tttcccacag gagttcctca
    14701 tgacgaatcg tatctctcgc ctcaaaactg cactgtttgc caatacccgt gaaatctcgc
    14761 tggagcgtgc gctgctttat accgccagcc atcggcaaac cgaaggcgaa ccggtgatat
    14821 tgcgccgggc gaaagcaaca gcgtatatcc ttgaacatgt tgaaatttcg attcgtgatg
    14881 aagaactgat tgccggtaac cgcaccgtaa aaccgcgcgc cgggattatg tcgccggaaa
    14941 tggaccctta ctggctgctg aaagagctgg atcaattccc gacgcgtccg caggaccgct
    15001 ttgctatcag cgaagaagat aaacgtatct accgtgaaga gttgttcccg tactgggaaa
    15061 aacgttcgat gaaagatttc attaacgggc agatgacgga tgaagtaaaa gccgcgacca
    15121 gcacgcagat tttcagcatc aaccagacgg ataaaggcca ggggcacatt attattgatt
    15181 acccgcgcct gctgaatcac gggctgggtg agctggtagc gcagatgcag caacattgtc
    15241 agcaacagcc ggagaatcac ttttatcagg cagcgctgtt actgctcgaa gcctcgcaga
    15301 aacacatttt gcgttacgcc gaactggcgg aaacgatggc ggcaagctgc actgatgccc
    15361 agcgtcgcga agaactgctg actattgcgg agatctcccg ccataacgcc gaacataagc
    15421 cgcagacgtt ctggcaggcg tgccagttat tctggtacat gaacatcatt ctgcaatacg
    15481 aatccaacgc cagttcacta tcgttggggc gcttcgacca gtatatgttg ccgttctatc
    15541 aggcatcatt aacccagggc gaagatccgg cgttcctgaa agaactgctc gaatctttat
    15601 gggtgaaatg caacgacatc gtgctgttgc gctccaccag tagcgcgcgt tatttcgccg
    15661 gtttcccgac cggctatacc gcactgctcg gcgggttaac cgagaacgga cgtagcgcgg
    15721 tgaacgtgct ttcgttcctt tgccttgacg cttatcaaag cgtgcaatta ccgcaaccga
    15781 acctcggcgt gcgcactaac gccttgatcg acacgccgtt cctgatgaaa accgcagaaa
    15841 ccattcgcct cggcaccggt attccgcaaa tctttaacga tgaagtggtg gtgccagcgt
    15901 tcctcaaccg tggcgtttcg ctggaagatg cacgcgacta ttccgtagtg ggctgtgtgg
    15961 aattatctat tcccggcaga acctacggct tgcatgacat cgcgatgttt aatctgctga
    16021 aagtgatgga aatctgcctg catgaaaatg aaggcaatgc tgcgctgact tatgaaggtt
    16081 tactggagca gatccgcgcc aagatcagcc actacatcac cctgatggtt gagggcagca
    16141 atatttgcga tatcggtcac cgtgactggg cacctgtacc gctgctctca tcgtttatca
    16201 gcgattgtct ggaaaaaggc agcgatatta ccgatggcgg cgcgcgttat aacttctccg
    16261 gcgtacaggg gatcggtatc gccaacctga gcgattctct ccatgcgttg aaagggatgg
    16321 tttttgagca acagcgttta agttttgacg aattgctgtc ggtattaaaa gccaacttcg
    16381 caacgccaga aggcgaaaaa gtccgtgctc gcttaattaa ccgcttcgag aaatacggta
    16441 acgatatcga cgaggtggat aacatcagcg ccgaactgtt acgccactac tgcaaagaag
    16501 tggaaaaata ccagaacccg cgcagcggct acttcacgcc gggatcgtat accgtttctg
    16561 ctcacgtccc gttgggatcg gtggttggcg cgacgccaga cggtcgtttt gccggggaac
    16621 aactggcaga cggcggcttg tcacccatgc tgggccagga cgcacaaggg ccaacggcgg
    16681 tactgaagtc agtcagtaag ctcgataata cgctgctgtc taacggtacg ttgctgaacg
    16741 tgaaattcac tccggcgact ctggaaggtg aagcgggatt acgcaaactg gccgacttct
    16801 tacgggcgtt tacccagctt aagttgcagc atattcagtt taacgtggtg aacgccgaca
    16861 cgttgcggga agcgcaacag cgcccacaag attatgccgg gctggtggtg cgcgttgccg
    16921 gatacagcgc cttctttgtc gaattgtcga aggagatcca ggatgacatc atccgccgga
    16981 cagcgcatca gctgtaacgt tgtggaaacg cgccgcaatg atgtggcgcg cattttcaac
    17041 attcagcgtt attcactgaa tgacggcgag ggcattcgta cggtggtctt ttttaaaggc
    17101 tgtccgcatc tttgcccatg gtgtgctaat ccggagtcga tctccggcaa aatccagacg
    17161 gtacgcagag aggcgaaatg tctgcactgt gcgaaatgtt tgcgtgatgc ggatgaatgc
    17221 ccctccgggg cgtttgaacg gattggccgc gatatcagcc ttgacgcgct ggagcgggaa
    17281 gtgatgaaag atgacatttt ctttcgcacg tccggcggcg gcgtcacgct ttctggcggc
    17341 gaagtgttaa tgcaggcgga gtttgctacc cgttttttac agcggctgcg gttgtggggc
    17401 gtgtcatgcg ccattgaaac tgccggagac gcgccagcca gcaagctgtt accgctggcg
    17461 aaattgtgcg atgaagtgtt gttcgattta aaaattatgg acgcgactca ggcgcgggat
    17521 gtggtgaaga tgaatctgcc acgcgtgctg gagaatctgc gtttgctggt gagtgagggc
    17581 gtcaacgtga tcccgcgttt accgctgatc cctggtttca cgctcagcca ggagaatatg
    17641 cagcaggctc tggacgtgct gatcccgctg aatatcaggc agatccatct gttaccgttt
    17701 catcagtacg gcgaaccgaa ataccgcctg ctggggaaaa catggtcgat gaaagaggtg
    17761 cctgcgccgt cgtcagctga tgtggcaacg atgcgcgaaa tggcagaacg ggccggattt
    17821 caggttaccg tgggaggtta aaatggcata cctggtggca gtaaccgcct gcgtcagtgg
    17881 cgtggcgcat acttatatgg cggcggaacg gctggaaaag ttgtgcctgt tagagaagtg
    17941 gggcgtcagc attgaaactc agggcgcgct gggaacggag aatcgtttag cggacgagga
    18001 tatccgccgg gcggatgttg cgctgttaat tacggatatc gagcttgccg gtgctgagcg
    18061 atttgaccat tgccgctatg tgcaatgcag catctacgct ttccttcgtg agccgcagcg
    18121 ggtgatgagc gcggtacgca aagtgctttc tgcgccgcag caaacccatc tcattctgga
    18181 gtagtcggtt tttctgtcag ttggctgtgg tactgccgac gatattccga cggtgagcgt
    18241 tcggtatttt tacgaaacag acggcagaag tagttgctgt cgacaaagcc gcaggcgtgc
    18301 gccacttctt ttaccttcag gtcatagcct ttcaataacg tcttagcgtg ctccagtcgc
    18361 gtgtgattca ggtattcgtt aaaaccagtg gcccccgttt tttgaaacag gtgcgagagg
    18421 taatttggcg aaatatagaa tgcctgtgcg acagattcgc gggtaagcgc ggaggcatag
    18481 cgttcgtcga tataatcacg aatagcttcg aacagtgcct ggctgcgtga ggcggtctgg
    18541 atttggctgc caagcaaatc gcggcagtgg ctaagcaggc tggcgacgat aagccttgcc
    18601 gtttgctgct cctgcgactg catttgcatt tcattgagcg tttgtagcag aaaagaacct
    18661 atgcgtgggc cgcgtcgggc gacgtgttgc ttcgccagat tttgatattg tttgccatcc
    18721 cattgcacga cgctgaagcc gagttgctgt ttgccaaaca gcacgctaaa ggtagtggcg
    18781 ggggcttgcc attgtggaaa attccagcca ccagcgggaa cgtacagcac atcgccggga
    18841 actaacgctg ctccagcgcc ggtatcaata aactcacctt ccagcacaat ttccagccgt
    18901 ggaaaatcga cctgatacgc aagatcgggc accgggccgt tgctgctggc aaagtaaatc
    18961 tggcgcaggg acagcgggcc gttgatcagg cgggagagca gatagctgac gtcgtgatac
    19021 atgttattcc cttaggtatg tagacgggaa aacagtaact ggcattatga aaaaaggcca
    19081 cctgaaaagt gtggcctgat gggagtgaga tgtaaacatt gcggcagttt cagtcgactt
    19141 gtattgccgg atgcggcgta aacgccttat ccggcctacc gccgcgatct gtaggccgga
    19201 taagacgcgt caagcgtcgc atccggcaaa taacatctgg ctgataatta ctgattaccc
    19261 acctgatcgc catacggcag agtgtcgtaa tcgatcagtg cgtttttctt atacgggtta
    19321 ccaatccagc gggtagtttc tttgaactgc ggattcacca ctgaacgggt tggatcgtaa
    19381 ccgtcgtaag ataagcccgc caaatctgac caggtgtgga tcagttccgc caggctgtat
    19441 ttacgatcaa catcctgtga gaaatcacgc ggatgagtcg cttgccattt ttctgacgtc
    19501 cacagcagga acggaatggt gtacatatga cgcgtcgggt tgtcttcatt acgcccctga
    19561 gttttgtgcg gcggcgtgtc gtaaacctct tcaccgtggt cagagaaata aaccaggaaa
    19621 ccgttcggat cggtcgcttt aaagtcttta atcaggctgg caaccacgtg atcgttgtac
    19681 aggttagcat tgtcataatc attatatgac tccagctctt ctgcgtttaa tcccggtgga
    19741 acatgctctg tattaccgtc aaatttgccc tgatcttccg gataacggta cttgtacttg
    19801 atatgcgtac ccagcagatg aacgatgatc agtttcttcg gcgcagggtc cttcagcact
    19861 tcctggaacg gcttcagcac gttggtgtcg tattcacgcg cactctgcgt acgttgctgg
    19921 ttcatgtagt actgcttatc ggtctggcgc gaaaataccg tcagcatggt attgcgggcg
    19981 gtcatcgtct gctggttggt gatccagaag gttttataac ccgcctgttt catcatgttc
    20041 atcagcgacg gctgcgtcag atacagatcc gggttctttt cattggcaaa ggtcagcgcc
    20101 tgttgcagga tttcaatggt gtacggacga gacgtaacca cgttattaaa cacggtcaga
    20161 ttcggatcgg ttttatgcag cgcatccagc tccggcgtgg tttcacgcgg ataaccgtac
    20221 agactcatgc gtccacgctg ggtcgattcg ccaattacca gcactaaggt gcgcggttcg
    20281 ttacccgatt catctttgaa attcgccagc ggcggcaagg cattattttc attcagtagc
    20341 tttgttagcg agtttagttg ctgacgatac tgataatagc cggtcaggaa ttgccacggt
    20401 gcggcaggct ccatacgcga tgccaggtta tccaacgttt tctcaaatgg cttgtttttg
    20461 ataaacgtat tcatggcgat cggatgcaga atcaagccat aaagtagggc aaaagagaca
    20521 acataacgcc acggctttgg aatatagacc gggcgcaggc gcgtccacag caaaactgcc
    20581 accgccgtat aggccagcgc gataagcaca atcttcaggc tgaaatactg gcttaaatac
    20641 tcgctggctt cgttggtgtt ggtttcaaac atcacaaaca gaacgctctg cgagaactcc
    20701 tgaccgtaga tgacgtagta gcacaacgct gccagagagg ccgcccatag caccacgccg
    20761 atcactgcgg caataatttt aatccgcttc ggaaagagga ataccgggat caaccacagc
    20821 gaactgaata acagcgagtc gcgaatgccg ttagtgccac tataaccact gatgtaaata
    20881 atggcctgta gcagagtaga gaaaaaccaa aagtagagca gtgcccaacc cagggccttc
    20941 cagctaaaaa ggggtttagc ctggacttct gtggaatgca tagtaagaac ctgtcttgaa
    21001 aaaaatatcg ccgaatgtaa cgacaattcc ttaaggatat ctgaagggat atccagattt
    21061 tgaataaaat gcagacagaa atatattgaa aacgagggtg ttagaacaga agtatttcag
    21121 aaaaccctcg cgcaaaagca cgagggtttg caggagagga agattagccg gtattacgca
    21181 tacctgccgc aatcccggca atagtgacca ttaacgcctg ctcgacgcga ggatccggct
    21241 catggccttc tttttctgcc tggcgggagc ggtgcagcaa ctcggcctgc aatacgttca
    21301 gcgggtcggt gtaaatattc cgtagctgaa tagactctgc aatccacggc agatcggcca
    21361 tcaggtgaga atcgttggca atcgccagca ccactttgat gtcttcttct tgcagattgc
    21421 gtaactcttt acctaacggc cacagtgctt tgtctaccag gcgttggtca tagtattccg
    21481 ccagccacag gtctgctttg gcgaagacca tctccagcat gccgagacgc gtcgagaaga
    21541 atggccaatc gcggcacatg gcttccagct cgctctgttt gccatcttcg accacttttt
    21601 gcagcgccgt acctgcaccc agccaggcgg ggagcatcag gcggttttgt gtccaggcaa
    21661 aaatccacgg aatagcgcgc agtgactcaa cgccaccggt cgggcgacgt tttgccggac
    21721 gtgaacccag cggtagtttg ccgagttcct gctccggcgt ggcagagcgg aagtaaggca
    21781 caaaatcttt gttttcacgc acgtagccgc ggtagacatc gcatgagata accgaaagct
    21841 catccataat gcgacgccag ctctctttcg gctctggcgg tggcagcagg ttggcttcca
    21901 gaatcgcccc ggtatacagc gaaaggctgc taacggtgat ttcaggcaga ccgtacttga
    21961 agcggatcat ctcgccctgt tcggtcacgc gcaggccgcc tttcaggctt cctggtggtt
    22021 gtgacagcag cgccgcatgg gcaggtgcgc cgccgcgacc aatggaaccg ccgcgaccgt
    22081 ggaacaacgt tagctcaata cccgcttttt cgcaggtttt gattaatgca tcctgtgcct
    22141 gatattgcgc ccaggaagct gccatcacgc ccgcatcttt tgctgagtcg gaatagccaa
    22201 tcatcaccat ctgtttgccc tgaatcaggc cgcgatacca gtcgatattc agcagctggg
    22261 tcatgacatc gttggcgttg ttcaggtcat cgagggtttc aaacagcgga gcaaccggca
    22321 tcgcaaaccc gatacccgct tctttcagca gcaggtggac agccagcacg tcggacggcg
    22381 ttttcgccat cgagatcacg taggcggcaa tggagccttg cggtgcttcg gcaatcacct
    22441 ggcaggtatc gagcacttcg cgcgtttcgg cgcttggttg ccagttgcgc ggtagaagcg
    22501 gacgtttgga gttcagttcg cggatcagga acgcctgttt gtcggcttct gaccagctct
    22561 cgtagtcgcc gataccgagg tagcgggtca gctcgcccag cgcttcggta tggcgggtgc
    22621 tctcctgacg gatatcaata cggaccagcg gtacgccgaa acatttcacg cggcgcaggg
    22681 tgtcgagcag atcgccgttg gcgataatac ccatgccaca cgcctgaagt gactggtagc
    22741 aagcgtagag cggttcccac agttcttcgt tttgtgtcag caggccttct ggttttggca
    22801 gttcttcgcc tttcaggcgc gcttccagcc atgcctgtgt cgccatcagg cgagaacgca
    22861 ggtttttcat cagatagcga tacggttctg cggcaccttc ttcgccaacc agcgccagca
    22921 gttcaggggt cgcttcaacc atcgacagtt cagaaaccag cacctgaata tctttcagga
    22981 acagatcggt ggctttccag cgactgagca gcaggacgtg gcgggtgata tcggcagtga
    23041 cgttcgggtt gccgtcgcgg tcaccgccca tccacgaggt aaaacggacc ggaacaaatt
    23101 cgacgggcag tttgtagccg agattctctt ccagttgttc gttcagttcg cgcaggtaat
    23161 tcggtacgcc ttgccacagg ctgttttcta ctacggcaaa gccccatttg gcttcatcta
    23221 ccgggcttgg acgcagctta cggatttcat ctgtatgcca tgactgggcg atcaactggc
    23281 gcaggcggcg catcagctgg ttatgttcgt agtcggcgat atctttgtta tcgagctgtt
    23341 ttaagcaggc gttcacttcc accattttgt ggatcagtgt acgacgggta atttcggttg
    23401 ggtgagccgt gaggaccagt tccagcgaca gcgattccac tgcttttttg atggtgtctt
    23461 cgctcagttc cggctggttt ttcagtttac gcagggtgcg ggcgatcact tccgggttgc
    23521 tggcagcttc gcctttcggc gaaatgctgt ggtattgctc agcggtgttg gccaggttca
    23581 ggaactgact aaacgcacgc gcaacgggca gcagctcgtc gttcgacaaa ttttgtaagg
    23641 tggtgagcaa ctcctggcgg ttagcatcat tgccagcgcg tgaagatttc gacaacttac
    23701 ggatagtttc tacgcgttca agaatgtgtt ctcccaacgc atccttgatg gtttctccca
    23761 gcactttgcc gagcatactg acattactac gcaatgcgga atattgttcg ttcatattac
    23821 cccagacacc ccatcttatc gtttgatagc cctgtatcct tcacgtcgca ttggcgcgaa
    23881 tatgctcggg ctttgctttt cgtcgtcttt tataaagcca cgtaaaagcg gtgacgtcaa
    23941 atgctgcgaa atcgcttcag caaacgaata aatagcagga atttacgcca ttaaattcat
    24001 gacgctttaa ataagcgtaa cttatgggaa tgttaaaaaa tcgcctcgag taacaccaaa
    24061 ggtgtaggtc ggataagatg cgtaagcatc gcatccgaca ttgtttggcc tacgttttaa
    24121 tgccagcaaa aatggtgaat cacctgggtg atcagttcgc gggtgggctt gataaacctc
    24181 gtctccagat attcatcagg ctgatgagcc tgattaattg agccaggccc cagaaccagc
    24241 gtcgggcata acgtttgaat aaacggcgct tcggtacagt aattcaccac ttcggtcttt
    24301 gctccgagca atttctcaac cacttcaacc agtttatgat tcggtgggca ttcatagcca
    24361 gggatcggcg gatgcagctc gtcgaccgtc agacgacccg gccagcgttc gctcactgga
    24421 gccaatgcat cgttgagcaa accattaagt tcattgagtg tcatgcctgg cagcggacga
    24481 atatccatat gcaactcaca gcaagcacaa atacggttag aagcgtcgcc accgtggata
    24541 tgcccgaggt tgagcgtagg gtatggcacg gtaaacgcgt cgtagtgata acgttctttc
    24601 aggttatcgc gcaattgcaa aatatgcccg atggcgtcgt gcatcagttc gatagcgtta
    24661 actccgcgtg ctggatcgct ggagtggccc gactggccct gaatacggat ggcgttagag
    24721 atatgacctt tatgcgcgcg taccggttgc agcgacgttg gctcgccaat gatggcgcaa
    24781 tccgggcgca gggcggtagt ttcggcaaaa taacgcgctc cggccatact ggtttcttca
    24841 tcggcagtcg ccagaatgta gagcggtttt gcaagcttcg tgacgtcgac atcgcgtagc
    24901 gcatcaagga taaacgcaaa aaagcctttc atgtcggcgg tgcctaagcc gtaaagcttg
    24961 ccgtcatgct ccgtcaacgt aaatggatcg cgcgtccagc gtccgtcatc aaatggcacc
    25021 gtatcggtat gccccgccag caacaaaccg ccagccccct gtccgcaact ggccagcata
    25081 ttaaatttgt tgcgagttcc tggcacaggc tgtacttcca cattgaagcc taaatcctta
    25141 aaccagtccg ccagcagagt gattaaatct gcattgcttt gatcgagtgc ctcttccgtg
    25201 gcgcttattg aaggtgtggc aatcagagca cggtaaatct cgataaatgg cggtaatttg
    25261 tttttcattg ttgacacacc tctggtcatg atagtatcaa tattcatgca gtatttatga
    25321 ataaaaatac actaacgttg agcgtaataa aacccaccag ccgtaaggtg aatgttttac
    25381 gtttaacctg gcaaccagac ataagaaggt gaatagcccc gatgttgaat acgctgattg
    25441 tgggtgccag cggctacgct ggcgcagagc tagtgaccta tgtaaatcgc catccgcata
    25501 tgaacataac cgctttgact gtttcagcgc aaagcaatga tgcgggaaag ttaatctccg
    25561 atttgcatcc gcagctaaaa ggcatcgttg atctgccgtt gcagccgatg tcggatatca
    25621 gcgagtttag cccaggggtg gacgtggtgt ttctcgccac cgcccatgaa gtcagccacg
    25681 atttagcgcc gcaatttctt gaagcaggct gtgtggtgtt cgacctttcc ggcgcgtttc
    25741 gtgttaacga tgtcgccttc tatgaaaaat attacggctt tacccatcaa tacccggaac
    25801 tgctggaaca ggcagcctac ggtctggcgg agtggtgcgg taataaatta aaagaagcga
    25861 acttgattgc cgtgccaggc tgttatccga cggcagcaca gctggcgctg aaaccgttga
    25921 ttgatgccga tcttcttgac ctcaatcagt ggccggtgat caacgccacc agcggcgtga
    25981 gcggtgcagg acgtaaggca gcgatttcaa atagtttttg tgaagttagc ctgcaaccgt
    26041 atggcgtctt tacccatcgc catcaaccag agatcgccac acacctcggt gctgacgtta
    26101 tcttcacccc gcatctgggc aatttcccgc gcggcattct cgaaaccatt acctgccgcc
    26161 tgaaaccggg tgtgagccag gtgcaagtgg cgcaagcgtt acagcaggcg tatgcccata
    26221 aaccactggt gcggctgtat gacaaaggcg ttccggcgct gaaaaatgtc gttggactgc
    26281 cattctgcga tatcggtttt gccgttcagg gcgagcatct gattattgtg gcgaccgaag
    26341 acaacttact gaaaggcgcg gcggcacaag cggtgcagtg cgccaatatt cgtttcggct
    26401 atgcggaaac gcagtctctt atttaagggt gcaatgatga atccattaat tatcaaactg
    26461 ggcggcgtac tgctggatag tgaggaggcg ctggaacgtc tgtttagtgc actggtgaat
    26521 tatcgtgagt cacatcagcg tccgctggtg attgtccacg gtggcggctg cgtagtggat
    26581 gagttgatga aagggctgaa tctgccggtg aaaaagaaaa atggcctgcg ggtgacgcct
    26641 gctgatcaga ttgacattat caccggagca ctggcgggaa cggcaaataa aaccctgctg
    26701 gcatgggcga agaaacatca gattgcggcc gtaggtttgt ttctcggtga cggcgacagc
    26761 gtcaaagtga cccagcttga tgaagagtta ggccatgttg gactggcgca gccaggttcg
    26821 cctaagctta tcaactcatt gctggagaac ggttatctgc cggtggtcag ctccattggc
    26881 gtaacagacg aagggcaact gatgaacgtc aatgccgatc aggcggcaac ggcgctggcg
    26941 gcaacgctgg gcgcggatct gattttgctc tccgacgtca gtggcattct cgacggcaaa
    27001 gggcaacgta ttgccgaaat gaccgccgcg aaagcagaac aactgattga gcagggtatt
    27061 attactgacg gcatgatagt gaaagtgaac gcggcgctgg atgctgcgcg cacgttgggc
    27121 cgtccggtag atatcgcctc ctggcgtcat gcggaacagc ttccggcact gtttaacggt
    27181 atgccgatgg gtacgcggat tttagcttaa gttttgttgg ccggaggcgc agctttccgg
    27241 cattgaattt caaaataagg aaacagagtt atggcacttt ggggcgggcg ttttacccag
    27301 gcagcagatc aacggttcaa acaattcaac gactcactgc gctttgatta ccgtctggcg
    27361 gagcaggata ttgttggctc tgtggcctgg tccaaagccc tggtaacggt cggcgtgtta
    27421 accgcagaag agcaggcgca actggaagag gcgctgaacg tgctgctgga agatgttcgc
    27481 gccaggccac aacaaatcct tgaaagcgac gccgaagata tccatagctg ggtggaaagc
    27541 aaactgatcg acaaagtggg tcagttaggc aaaaaactgc ataccgggcg tagccgtaat
    27601 gatcaggtag caactgacct gaaactgtgg tgcaaagata ccgttagcga gttgctgacg
    27661 gctaaccggc agttacaatc cgctctggtc gagaccgcgc agaacaatca ggacgcggtg
    27721 atgccgggtt acactcacct gcaacgcgcc cagccggtga cgttcgctca ctggtgcctg
    27781 gcctacgttg agatgctggc gcgtgatgaa agccgtttgc aggacgcgct taagcgtctg
    27841 gatgtcagcc cgctaggctg tggcgcactg gcaggaacgg cctatgaaat cgaccgtgaa
    27901 cagttagcag gctggctggg ctttgcttcg gcgacccgta acagtctcga cagcgtttct
    27961 gaccgtgacc atgtgctgga actgctttct gctgccgcta tcggcatggt gcatctgtcg
    28021 cgttttgctg aagatctgat tttctttaac accggcgaag cggggtttgt ggagctttct
    28081 gaccgtgtga cttccggttc atcattaatg ccgcagaaga aaaacccgga tgcgctggag
    28141 ctgattcgcg gtaaatgcgg tcgggtgcag ggggcgttaa ccggcatgat gatgacgctg
    28201 aaaggtttgc cgctggctta caacaaagat atgcaggaag acaaagaagg tctgttcgac
    28261 gcgctcgata cctggctgga ctgcctgcat atggcggcgc tggtgctgga cggcattcag
    28321 gtgaaacgtc cgcgttgcca ggaagcggcg cagcagggtt acgccaacgc tactgaactg
    28381 gcggattatc tggtggcgaa aggcgtaccg ttccgcgagg cgcaccatat tgtgggtgaa
    28441 gctgtggtgg aagccattcg tcagggcaaa gcgttggaag atctgccgct cagtgagttg
    28501 cagaaattca gccaggtgat tggcgaagat gtctatccga ttctgtcgct gcaatcgtgc
    28561 ctcgacaagc gtgctgcaaa aggcggcgtc tcaccgcagc aggtggcgca ggcgattgct
    28621 tttgcgcaag ctcggttaga gtagtttgtt tatattgctc ccctgaaaat ggggagctta
    28681 ccacgcctac aaagtgcgac acttcgtagg cggataaggc gtttacgccg catcaggcag
    28741 aaatttgtca gtattctggt gaccttaaat ttaacgccca accagaacca ttatttgtca
    28801 cattcctttt tattcttgcg tcttctttac cttcactctc cccata
//
LOCUS       DAZFLD010000038        28840 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.038, whole genome shotgun sequence.
ACCESSION   DAZFLD010000038 DAZFLD010000000
VERSION     DAZFLD010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28840)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28840
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.038"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..544)
                     /gene="yjgL"
                     /locus_tag="ACHKBL_001402"
     CDS             complement(<1..544)
                     /gene="yjgL"
                     /locus_tag="ACHKBL_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjgL"
                     /protein_id="HFO5818293.1"
                     /translation="MSKISDLNYSQHITLADNFKQKSEVLNTWRVGMNDFARIAGGQD
                     SRRNILSPGAFLEFLAKIFTLGYVDFSKRSNEAGRNMMAHIESSSYSKDTDGNEKMKF
                     YMNNPEGERADLSKVEIEITLSTITTMGTRQGHTAIIFQQSDGSTNRYEGKSFERKDD
                     SSLHLITNKVLACYQREANKE"
     gene            complement(667..1119)
                     /gene="tabA"
                     /locus_tag="ACHKBL_001403"
     CDS             complement(667..1119)
                     /gene="tabA"
                     /locus_tag="ACHKBL_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin biofilm protein TabA"
                     /protein_id="HFO5818294.1"
                     /translation="MIIGNIHNLQPWLPQELRQAIEHIKANVTAETPKGKHDIEGNRL
                     FYLISEDMTEPYEARRAEYHARYLDIQIVLKGQEGMTFSTQPAGTPDTDWLADKDIAF
                     LPEGVDEKTVILNEGDFVVFYPGEVHKPLCAVGAPARVRKAVVKMLMA"
     gene            complement(1264..1857)
                     /gene="bdcR"
                     /locus_tag="ACHKBL_001404"
     CDS             complement(1264..1857)
                     /gene="bdcR"
                     /locus_tag="ACHKBL_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709951.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFO5818295.1"
                     /translation="MVTKKQSRVPGRPRRFAPEQAVSAAKVLFHQKGFDAVSVSEVTD
                     YLGINPPSLYAAFGSKAGLFSRVLNEYVGTEAIPLADILRDDRPVGECLVEVLKEAAR
                     RYSQNGGCAGCMVLEGIHSHDPQARDIAIQYYHAAETTIYDYIARRHPQSAQCVTDFM
                     STVMSGLSAKAREGHSIEQLCATAALAGEAIKTILEE"
     gene            1928..2641
                     /gene="bdcA"
                     /locus_tag="ACHKBL_001405"
     CDS             1928..2641
                     /gene="bdcA"
                     /locus_tag="ACHKBL_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFO5818296.1"
                     /translation="MGAFTGKTVLILGGSRGIGAAIVRRFVTDGANVRFTYAGSKDAA
                     ERLAQETGATAVFTDSADRDAVIDVVRKSGALDILVVNAGIGVFGDALELNADDIDRL
                     FKINIHAPYHASVEAARQMPEGGRILIIGSVNGDRMPVAGMAAYAASKSALQGMARGL
                     ARDFGPRGITINVVQPGPIDTDANPANGPMRDMLHSFMAIKRHGQPEEVAGMVAWLAG
                     PEASFVTGAMHTIDGAFGA"
     gene            2773..3168
                     /gene="yjgH"
                     /locus_tag="ACHKBL_001406"
     CDS             2773..3168
                     /gene="yjgH"
                     /locus_tag="ACHKBL_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFO5818297.1"
                     /translation="MVERTAVFPAGRHSLYAEHRYSAAIRSGDLLFVSGQVGSREDGT
                     PEPDFQQQVRLAFDNLHATLAAAGCTFDDIIDVTSFHTDPEKQFEDIMTVKNEIFSAP
                     PYPNWTAVGVTWLAGFDFEIKVIARIPEL"
     gene            3449..3583
                     /gene="pyrL"
                     /locus_tag="ACHKBL_001407"
     CDS             3449..3583
                     /gene="pyrL"
                     /locus_tag="ACHKBL_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyr operon leader peptide"
                     /protein_id="HFO5818298.1"
                     /translation="MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCPGVRR
                     "
     gene            3587..4522
                     /gene="pyrB"
                     /locus_tag="ACHKBL_001408"
     CDS             3587..4522
                     /gene="pyrB"
                     /locus_tag="ACHKBL_001408"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HFO5818299.1"
                     /translation="MANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKV
                     IASCFFEASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDA
                     IVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAM
                     VGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEE
                     VMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIAT
                     DVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL"
     gene            4535..4996
                     /gene="pyrI"
                     /locus_tag="ACHKBL_001409"
     CDS             4535..4996
                     /gene="pyrI"
                     /locus_tag="ACHKBL_001409"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009347 - aspartate carbamoyltransferase
                     complex [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HFO5818300.1"
                     /translation="MTHDNKLQVEAIKRGTVIDHIPAQIGFKLLSLFKLTETDQRITI
                     GLNLPSGEMGRKDLIKIENTFLSEDQVDQLALYAPQATVNRIDNYEVVGKSRPSLPER
                     IDNVLVCPNSNCISHAEPVSSSFAVRKRANDIALKCKYCEKEFSHNVVLAN"
     gene            5069..5455
                     /gene="ridA"
                     /locus_tag="ACHKBL_001410"
     CDS             5069..5455
                     /gene="ridA"
                     /locus_tag="ACHKBL_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminobutanoate/2-iminopropanoate deaminase"
                     /protein_id="HFO5818301.1"
                     /translation="MSKTIATENAPAAIGPYVQGVDLGNMIITSGQIPVNPKTGEVPA
                     DVAAQARQSLDNVKAIVEAAGLKVGDIVKTTVFVKDLNDFATVNATYEAFFTEHNATF
                     PARSCVEVARLPKDVKIEIEAIAVRR"
     gene            complement(5661..8357)
                     /gene="mgtA"
                     /locus_tag="ACHKBL_001411"
     CDS             complement(5661..8357)
                     /gene="mgtA"
                     /locus_tag="ACHKBL_001411"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HFO5818302.1"
                     /translation="MFKEIFTRFIRHLPSRLVHRDPLPGAQQTVNTAVPPSLSAHCLK
                     MAVMPEEELWKTFDTHPEGLNQAEVESAREQHGENKLPAQQPSPWWVHLWVCYRNPFN
                     ILLTILGAISYATEDLFAAGVIALMVAISTLLNFIQEARSTKAADALKAMVSNTATVL
                     RVINDKGENGWLEIPIDQLVPGDIIKLAAGDMIPADLRILQARDLFVAQASLTGESLP
                     VEKAATTRQPEHSNPLECDTLCFMGTTVVSGTAQAMVIATGANTWFGQLAGRVSEQES
                     EPNAFQQGISRVSMLLIRFMLVMAPVVLLINGYTKGDWWEAALFALSVAVGLTPEMLP
                     MIVTSTLARGAVKLSKQKVIVKHLDAIQNFGAMDILCTDKTGTLTQDKIVLENHTDIS
                     GKTSERVLHSAWLNSHYQTGLKNLLDTAVLEGTDEESARSLASRWQKIDEIPFDFERR
                     RMSVVVAENTEHHQLVCKGALQEILNVCSQVRHNGEIVPLDDIMLRKIKRVTDTLNRQ
                     GLRVVAVATKYLPAREGDYQRADESDLILEGYIAFLDPPKETTAPALKALKASGITVK
                     ILTGDSELVAAKVCHEVGLDAGEVVIGSDIETLSDDELANLAQRTTLFARLTPMHKER
                     IVTLLKREGHVVGFMGDGINDAPALRAADIGISVDGAVDIAREAADIILLEKSLMVLE
                     EGVIEGRRTFANMLKYIKMTASSNFGNVFSVLVASAFLPFLPMLPLHLLIQNLLYDVS
                     QVAIPFDNVDDEQIQKPQRWNPADLGRFMIFFGPISSIFDILTFCLMWWVFHANTPET
                     QTLFQSGWFVVGLLSQTLIVHMIRTRRVPFIQSCASWPLMIMTVIVMIVGIALPFSPL
                     ASYLQLQALPLSYFPWLVAILAGYMTLTQLVKGVYSRRYGWQ"
     gene            complement(8498..8551)
                     /gene="mgtL"
                     /locus_tag="ACHKBL_001412"
     CDS             complement(8498..8551)
                     /gene="mgtL"
                     /locus_tag="ACHKBL_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mgtA regulatory leader peptide MgtL"
                     /protein_id="HFO5818303.1"
                     /translation="MEPDPTPLPRRRLKLFR"
     gene            8736..9683
                     /gene="treR"
                     /locus_tag="ACHKBL_001413"
     CDS             8736..9683
                     /gene="treR"
                     /locus_tag="ACHKBL_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor TreR"
                     /protein_id="HFO5818304.1"
                     /translation="MQNRLTIKDIARLSGVGKSTVSRVLNNESGVSQRTRERVEAVMN
                     QHGFSPSRSARAMRGQSDKVVAIIVTRLDSLSENLAVQTMLPAFYEQGYDPIMMESQF
                     SPQLVAEHLGVLKRRNIDGVVLFGFTGITEEMLAYWQSSLVLLARDAKGFASVCYDDE
                     GAIKILMQRLYDQGHRNISYLGVPHSDVTTGKRRHEAYLAFCKAHKLHPVAALPGLAM
                     KQGYENVAKVITPETTALLCATDTLALGASKYLQEQRIDTLQLASVGNTPLMKFLHPE
                     IVTVDPGYAEAGRQAACQLIAQVTGRSEPQQIIIPATLS"
     gene            9802..11223
                     /gene="treB"
                     /locus_tag="ACHKBL_001414"
     CDS             9802..11223
                     /gene="treB"
                     /locus_tag="ACHKBL_001414"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015574 - trehalose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015771 - trehalose transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
                     /protein_id="HFO5818305.1"
                     /translation="MMSKINQTDIDRLIELVGGRGNIATVSHCITRLRFVLNQPANAR
                     PKEIEQLPMVKGCFTNAGQFQVVIGTNVGDYYQALIASTGQAQVDKEQVKKAARQNMK
                     WHEQLISHFAEIFFPLLPALISGGLILGFRNVIGDLPMSNGQTLAQMYPSLQTIYDFL
                     WLIGEAIFFYLPVGICWSAVKKMGGTPILGIVLGVTLVSPQLMNAYLLGQQLPEVWDF
                     GMFSIAKVGYQAQVIPALLAGLALGVIETRLKRIVPDYLYLVVVPVCSLILAVFLAHA
                     LIGPFGRMIGDGVAFAVRHLMTGSFAPIGAALFGFLYAPLVITGVHQTTLAIDLQMIQ
                     SMGGTPVWPLIALSNIAQGSAVIGIIISSRKHNEREISVPAAISAWLGVTEPAMYGIN
                     LKYRFPMLCAMIGSGLAGLLCGLNGVMANGIGVGGLPGILSIQPSYWQVFALAMAIAI
                     IIPIVLTSFIYQRKYRLGTLDIV"
     gene            11273..12928
                     /gene="treC"
                     /locus_tag="ACHKBL_001415"
     CDS             11273..12928
                     /gene="treC"
                     /locus_tag="ACHKBL_001415"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008788 - alpha,alpha-phosphotrehalase
                     activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HFO5818306.1"
                     /translation="MTNLPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHK
                     LGVDAIWLTPFYVSPQVDNGYDVANYTAIDPTYGTLDDFDELVTQAKSRGIRIILDMV
                     FNHTSTQHAWFREALNKASPYRQFYIWRDGESETPPNNWRSKFGGSAWRWHAESEQYY
                     LHLFAPEQADLNWENPAVRAELKKVCEFWADRGVDGLRLDVVNLISKDPRFPDDLDGD
                     GRRFYTDGPRAHEFLHEMNRDVFTPRRLMTVGEMSSTSLEHCQRYATLTGSELSMTFN
                     FHHLKVDYPSGEKWTLAKPDFVALKTLFRHWQQGMHNVAWNALFWCNHDQPRIVSRFG
                     DEGEYRVPAAKMLAMVLHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELR
                     NDGRDADELLAILASKSRDNSRTPMQWSNGDNAGFTAGEPWIGLGDNYQEINVEAALA
                     DESSVFYTYQKLIALRKQEAVLTWGDYQDLLPNSPVLWCYRREWKGQTLLVIANLSRE
                     TQPWQPGKMLGNWQLVMHNYEEASPQPCAMTLRPFEAVWWLQK"
     gene            13313..15451
                     /gene="nrdD"
                     /locus_tag="ACHKBL_001416"
     CDS             13313..15451
                     /gene="nrdD"
                     /locus_tag="ACHKBL_001416"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HFO5818307.1"
                     /translation="MTPHVMKRDGCKVPFKSERIKEAILRAAKAAEVDDADYCATVAA
                     VVSEQMQGRSQVDINEIQTAVENQLMSGPYKQLARAYIEYRHDRDIEREKRGRLNQEI
                     RGLVEQTNASLLNENANKDSKVIPTQRDLLAGIVAKHYARQHLLPRDVVQAHERGDIH
                     YHDLDYSPFFPMFNCMLIDLKGMLTQGFKMGNAEIEPPKSISTATAVTAQIIAQVASH
                     IYGGTTINRIDEVLAPFVTASYNKHRKTAEEWNIPDAEGYANSRTIKECYDAFQSLEY
                     EVNTLHTANGQTPFVTFGFGLGTSWESRLIQESILRNRIAGLGKNRKTAVFPKLVFAI
                     RDGLNHKKGDPNYDIKQLALECASKRMYPDILNYDQVVKVTGSFKTPMGCRSFLGVWE
                     NENGEQIHDGRNNLGVISLNLPRIALEAKGDEATFWKLLDERLVLARKALMTRIARLE
                     GVKARVAPILYMEGACGVRLNADDDVSEIFKNGRASISLGYIGIHETINALFGGEHVY
                     DNEQLRAKGIAIVERLRQAVDQWKEETGYGFSLYSTPSENLCDRFCRLDTAEFGVVPG
                     VTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPLANGGFICYGEYPNIQHNLKALEDV
                     WDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGFTCPKCGNHDASRVSVTRRVCGY
                     LGSPDARPFNAGKQEEVKRRVKHLGNGQIG"
     gene            15609..16073
                     /gene="nrdG"
                     /locus_tag="ACHKBL_001417"
     CDS             15609..16073
                     /gene="nrdG"
                     /locus_tag="ACHKBL_001417"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate
                     reductase-activating protein"
                     /protein_id="HFO5818308.1"
                     /translation="MNYHQYYPVDIVNGPGTRCTLFVSGCVHECPGCYNKSTWRVNSG
                     QPFTKAMEDQIINDLNDTRIKRQGISLSGGDPLHPQNVPDILKLVQRIRAECPGKDIW
                     VWTGYKLDELNAAQMQVVDLINVLVDGKFVQDLKDPSLIWRGSSNQVVHHLR"
     gene            complement(16118..16504)
                     /gene="cybC"
                     /locus_tag="ACHKBL_001418"
     CDS             complement(16118..16504)
                     /gene="cybC"
                     /locus_tag="ACHKBL_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
                     /protein_id="HFO5818309.1"
                     /translation="MRKSLLAILAVSSLVFSSASFAADLEDNMETLNDNLKVVEKADN
                     AAQVKDALTKMRAAALDAQKATPPKLEGKSPDSPEMKDFRHGFDILVGQIDDALKLAN
                     EGKVKEAQAAAEQLKTTRNAYHQKYR"
     gene            complement(16687..18039)
                     /gene="pmbA"
                     /locus_tag="ACHKBL_001419"
     CDS             complement(16687..18039)
                     /gene="pmbA"
                     /locus_tag="ACHKBL_001419"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HFO5818310.1"
                     /translation="MALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTT
                     GISVSTRYGEVENVEFNSDGALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARY
                     TSPDPCAGVADKELLAFDAPDLDLFHPAEVSPDEAIELAARAEQAALQADKRITNTEG
                     GSFNSHYGVKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMSDLQT
                     PEWVGADCARRTLSRLSPRKLSTMKAPVIFANEVATGLFGHLVGAIAGGSVYRKSTFL
                     LDSLGKQILPDWLTIEEHPHLLKGLASTPFDSEGVRTERRDIIKDGILTQWLLTSYSA
                     RKLGLKSTGHAGGIHNWRIAGQGLSFEQMLKEMGTGLVVTELMGQGVSAITGDYSRGA
                     AGFWVENGEIQYPVSEITIAGNLKDMWRNIVTVGNDIETRSNIQCGSVLLPEMKIAGQ
                     "
     gene            18133..18684
                     /gene="yjgA"
                     /locus_tag="ACHKBL_001420"
     CDS             18133..18684
                     /gene="yjgA"
                     /locus_tag="ACHKBL_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis factor YjgA"
                     /protein_id="HFO5818311.1"
                     /translation="MTKQPEDWLDDVPGDDIEDEDDEIIWVSKSEIKRDAEELKRLGA
                     EIVDLGKNALDKIPLDADLRAAIELAQRIKMEGRRRQLQLIGKMLRQRDVEPIRQALD
                     KLKNRHNQQVVLFHKLENLRDRLIEQGDDAIAEVLNLWPDADRQQLRTLIRNAKKEKE
                     GNKPPKSARQIFQYLRELAENEG"
     gene            complement(18842..20215)
                     /gene="mpl"
                     /locus_tag="ACHKBL_001421"
     CDS             complement(18842..20215)
                     /gene="mpl"
                     /locus_tag="ACHKBL_001421"
                     /EC_number="6.3.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HFO5818312.1"
                     /translation="MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEK
                     QGIELIQGYDASQLDPQPDLVIIGNAMTRGNPCVEAVLEKNIPYMSGPQWLHDFVLRD
                     RWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSARLGESDFFVIEAD
                     EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRII
                     WPENDINLKQTMAMGCWSEQELVGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLV
                     GEHNMHNGLMAIAAARHVGVAPADAANALGSFINARRRLELRGEANGVTVYDDFAHHP
                     TAILATLAALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPAHI
                     PWQVAEVAEACVQPAHWSGDVDTLADMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLA
                     KKAEAAQ"
     gene            20391..21389
                     /gene="fbp"
                     /locus_tag="ACHKBL_001422"
     CDS             20391..21389
                     /gene="fbp"
                     /locus_tag="ACHKBL_001422"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HFO5818313.1"
                     /translation="MKTLGEFIVEKQHEFSHATGELTALLSAIKLGAKIIHRDINKAG
                     LVDILGASGAENVQGEVQQKLDLFANEKLKAALKARDIVAGIASEEEDEIVVFEGCEH
                     AKYVVLMDPLDGSSNIDVNVSVGTIFSIYRRVTPVGTPVTEEDFLQPGNKQVAAGYVV
                     YGSSTMLVYTTGCGVHAFTYDPSLGVFCLCQERMRFPEKGKTYSINEGNYIKFPNGVK
                     KYIKFCQEEDKSTNRPYTSRYIGSLVADFHRNLLKGGIYLYPSTASHPDGKLRLLYEC
                     NPMAFLAEQAGGKASDGKERILDIIPETLHQRRSFFVGNDHMVEDVERFIREFPDA"
     gene            complement(21422..22417)
                     /gene="yjfF"
                     /locus_tag="ACHKBL_001423"
     CDS             complement(21422..22417)
                     /gene="yjfF"
                     /locus_tag="ACHKBL_001423"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, permease
                     protein YjfF"
                     /protein_id="HFO5818314.1"
                     /translation="MIKRNLPLMITIGVFVLGYIYCLTQFPGFASTRVICNILTDNAF
                     LGIIAVGMTFVILSGGIDLSVGSVIAFTGVFLAKVIGDFGLSPLLAFPLVLVMGCAFG
                     AFMGLLIDALKIPAFIITLAGMFFLRGVSYLVSEESIPINHPVYDTLSSLAWKIPGGG
                     RLSAMGLLMLAVVVIGIFLAHRTRFGNQVYAIGGNATSANLMGISTRSTTIRIYMLST
                     GLATLAGIVFSIYTQAGYALAGVGVELDAIASVVIGGTLLSGGVGTVLGTLFGVAIQG
                     LIQTYINFDGTLSSWWTKIAIGILLFIFIALQRGLTVLWENRQSSPVTRVNIAQR"
     gene            complement(22404..23426)
                     /gene="ytfT"
                     /locus_tag="ACHKBL_001424"
     CDS             complement(22404..23426)
                     /gene="ytfT"
                     /locus_tag="ACHKBL_001424"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, ATP-binding
                     protein YtfT"
                     /protein_id="HFO5818315.1"
                     /translation="MPQSLPDTTPPKRRFRWPTGMPQLAALLLVLLVDSLVAPHFWQV
                     VLQDGRLFGSPIDILNRAAPVALLAIGMTLVIATGGIDLSVGAVMAIAGATTAAMTVA
                     GFSLPIVLLSALGTGILAGLWNGILVAILKIQPFVATLILMVAGRGVAQLITSGQIVT
                     FNSPDLSWFGSGSLLFLPTPVIIAVLTLLLFWLLTRKTALGMFIEAVGINIRAAKNAG
                     VNTRIIVMLTYVLSGLCAAIAGIIVAADIRGADANNAGLWLELDAILAVVIGGGSLMG
                     GRFNLLLSVVGALIIQGMNTGILLSGFPPEMNQVVKAVVVLCVLIVQSQRFISLIKGV
                     RNRDKT"
     gene            complement(23440..24942)
                     /gene="ytfR"
                     /locus_tag="ACHKBL_001425"
     CDS             complement(23440..24942)
                     /gene="ytfR"
                     /locus_tag="ACHKBL_001425"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, ATP-binding
                     protein YtfR"
                     /protein_id="HFO5818316.1"
                     /translation="MTTDQHQEILRTEGLSKFFPGVKALDNVDFSLRRGEIMALLGEN
                     GAGKSTLIKALTGVYHADRGTIWLEGQAISPKNTAHAQQLGIGTVYQEVNLLPNMSVA
                     DNLFIGREPKRFGLLRRKEMEKRATELMASYGFSLDVREPLNRFSVAMQQIVAICRAI
                     DLSAKVLILDEPTASLDTQEVELLFGLMRQLRDRGVSLIFVTHFLDQVYQVSDRITVL
                     RNGSFVGCRETRELPQIELVKMMLGRELDTHALQRAGRTLLSDKPVAAFKNYGKKGTI
                     APFDLEVRPGEIVGLAGLLGSGRTETAEVIFGIKPADSGTALIKGKQQTLRSPHQASV
                     LGIGFCPEDRKTDGIIAAASVRENIILALQAQRGWLRPISRKEQQEIAERFIRQLGIR
                     TPSTEQPIEFLSGGNQQKVLLSRWLLTRPQFLILDEPTRGIDVGAHAEIIRLIETLCA
                     DGLALLVISSELEELVGYADRVIIMRDRKQVAEIPLAELSVPAIMNAIAA"
     gene            complement(25082..26038)
                     /gene="ytfQ"
                     /locus_tag="ACHKBL_001426"
     CDS             complement(25082..26038)
                     /gene="ytfQ"
                     /locus_tag="ACHKBL_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter
                     substrate-binding protein YtfQ"
                     /protein_id="HFO5818317.1"
                     /translation="MWKRLLVVSAVSAAMSSMALAAPLTVGFSQVGSESGWRAAETNV
                     AKSEAEKRGITLKIADGQQKQENQIKAVRSFVAQGVDAIFIAPVVATGWEPVLKEAKD
                     AEIPVFLLDRSIDVKDKSLYMTTVTADNILEGKLIGDWLVKEVNGKPCNVVELQGTVG
                     ASVAIDRKKGFAEAIKNAPNIKIIRSQSGDFTRSKGKEVMESFIKAENNGKNICMVYA
                     HNDDMVIGAIQAIKEAGLKPGKDILTGSIDGVPDIYKAMIDGEANASVELTPNMAGPA
                     FDALEKYKKDGTMPEKLTLTKSTLYLPDTAKEELEKKKNMGY"
     gene            26348..26878
                     /gene="ppa"
                     /locus_tag="ACHKBL_001427"
     CDS             26348..26878
                     /gene="ppa"
                     /locus_tag="ACHKBL_001427"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HFO5818318.1"
                     /translation="MSLLNVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDR
                     FMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSVIRCRPVGVLKMTDEAGE
                     DAKLIAVPHTKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDLEKGKWVKVEGWENAE
                     AAKAEIVASFERAKNK"
     gene            complement(27251..27757)
                     /gene="mug"
                     /locus_tag="ACHKBL_001428"
     CDS             complement(27251..27757)
                     /gene="mug"
                     /locus_tag="ACHKBL_001428"
                     /EC_number="3.2.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000700 - mismatch base pair DNA
                     N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
                     /protein_id="HFO5818319.1"
                     /translation="MVEDILAPGLRVVFCGINPGLSSAGTGFPFAHPANRFWKVIYQA
                     GFTDRQLKPQEAQHLLDYRCGVTKLVDRPTVQANEVSKQELHAGGRKLIEKIEDYQPQ
                     ALAILGKQAYEQGFSQRGAQWGKQTLTIGSTQIWVLPNPSGLSRVSLEKLVEAYRELD
                     QALVVRGR"
     gene            27882..27957
                     /locus_tag="ACHKBL_001429"
     tRNA            27882..27957
                     /locus_tag="ACHKBL_001429"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:27915..27917,aa:Ile,seq:cat)
     gene            complement(28057..>28840)
                     /locus_tag="ACHKBL_001430"
     CDS             complement(28057..>28840)
                     /locus_tag="ACHKBL_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HFO5818320.1"
                     /translation="TSIRHILAVRKSTLLQIDTLIRQLAEISAMTESIGGKTALDWAM
                     KQDFRCGCWLMEKPETAMKAITRNLDREIWRDLMQRSGMLSLMDAQARDTWYRSLEYD
                     NFPEISEANILSTFEQLHQNKNEVFERGVINVFRGLSWDYKTNSPCKFGSKIIVNNLV
                     RWDRWGFHLNSGQQADRLADLERMLHLFSGKPIPDNRENITIHLDDHIQSVQGKEDYE
                     DEMFSIRYFKKGSAHITFRKPELVDRLNDIIAKHYPGQLSAI"
BASE COUNT         7092 a         7669 c         7176 g         6903 t
ORIGIN      
        1 tttccttgtt agcttcgcgt tggtaacacg ccagaacctt gttagtaatc aggtgtaatg
       61 aactatcatc ttttctttca aaagacttcc cttcataacg attagtcgaa ccatcagatt
      121 gttgaaatat aatagccgta tgtccttgac gagtccccat agtagtgata gtggaaagtg
      181 taatttctat ttccacctta gataaatccg ctcgttcccc ttcaggatta ttcatgtaaa
      241 acttcatttt ttcattgcca tcagtatctt tagaataaga tgaggactca atatgagcca
      301 tcatatttct acccgcttcg ttggagcgtt tgctaaaatc cacataaccc agggtaaata
      361 tctttgccaa aaactctaaa aatgctccag gagaaagaat attccttctg ctatcctgcc
      421 ccccggcaat acgggcaaaa tcattcattc caacacgcca ggtatttaaa acttcacttt
      481 tttgtttaaa attgtcggct aatgtaatgt gttgagaata attcaaatct gatattttgc
      541 tcatgacgaa ctccttgagt taataagaac gtctaagcat tacaaatttt actcacaaaa
      601 aaatatcaaa aaacgctctt ttcacaaact cgccgtaaac ctggagttat ttacggcgaa
      661 aagtcatcac gccatcagca tcttcactac tgctttgcga acccgggctg gcgcgcccac
      721 tgcgcacagc ggtttatgca cttcccccgg ataaaacaca acaaaatcac cttcattaag
      781 gataactgtt ttctcatcaa cgccttccgg caaaaatgcg atgtctttat cagctaacca
      841 gtcggtatcc ggcgtacctg caggttgtgt gctgaaggtc atgccttcct gaccttttaa
      901 cacaatctga atgtcgagat agcgggcatg gtactccgca cggcgagctt cgtacggctc
      961 ggtcatatct tccgagataa gataaaacag acgattgcct tcgatatcgt gcttgccctt
     1021 gggcgtttct gccgtaacgt ttgctttgat atgctcaatc gcctggcgta actcttgggg
     1081 tagccacggc tgaagattat gaatatttcc gatgatcatc ttttccactc cttaattaaa
     1141 acaacgtttc atttctatcg ttatacgaaa agatgatcaa ttccgctagc cttaacgcgt
     1201 gaattattgt cttatcacaa cttatcggca ggcgcgttcc cgcctttcgg gtcaaggccc
     1261 gcatcactcc tcgagaatag tttttatcgc ttcccccgcc agggcagctg ttgcacagag
     1321 ttgttcgatg gagtgcccct cccgtgcctt cgcagaaagc cctgacatca cggtactcat
     1381 aaaatcagtc acacattgtg cgctttgtgg atgccgcctg gcgatatagt cataaatggt
     1441 cgtttctgcg gcgtgataat attgaatggc aatatcacgc gcttgtggat catgactatg
     1501 aataccttca agaaccatac agccagcgca gccgccgttt tggctatatc tgcgcgccgc
     1561 ttcttttaat acctcaacca ggcactcgcc taccggacga tcatcacgaa gaatatcggc
     1621 aagcggaata gcttccgtac cgacatattc attgagtaca cgactaaaca acccagcctt
     1681 actgccaaaa gccgcgtaga ggctcggggg gttaatacca agataatcag taacttcaga
     1741 gacacttact gcatcgaaac ctttttggtg aaaaagcact tttgccgcag agactgcctg
     1801 ctcaggagcg aaacgtctgg ggcgacctgg aacgcgagat tgttttttag tgaccattac
     1861 aaaacttgtt gacagaaagt taaaacagtt ttgtaatgca tgttacataa taaatcaagg
     1921 agtccttatg ggcgctttta ccggtaagac agttctcatc ctcggtggta gccgtggaat
     1981 cggtgccgct atcgtacgtc gtttcgtcac cgatggagcc aatgtacgat tcacctatgc
     2041 ggggtcgaaa gatgccgctg aacgtctggc acaagagact ggagcgacag cagtattcac
     2101 agatagtgct gacagagacg ctgttattga tgtcgttcgt aagagcggcg cattggatat
     2161 cctggtggta aatgcaggta ttggcgtctt tggcgatgcc ctggaattaa atgccgacga
     2221 tattgatcgc cttttcaaaa tcaatattca tgctccttat catgcctctg ttgaagccgc
     2281 ccggcagatg cccgaaggcg ggcgcatctt aatcatcggc tccgtgaatg gcgatcgtat
     2341 gcctgttgca ggcatggctg cttatgccgc cagtaaatct gccctgcaag gcatggcgcg
     2401 cgggctggcc cgtgattttg gaccgcgagg gatcaccatc aacgtcgtcc agccaggacc
     2461 aattgatacc gacgctaatc ccgccaacgg gccaatgcgc gatatgttgc atagttttat
     2521 ggccatcaaa agacatgggc aaccggaaga ggtcgctggt atggtcgcat ggttagcagg
     2581 gccagaagcc agttttgtta ccggcgcgat gcataccatt gatggcgcgt ttggcgcata
     2641 accgactacg ctcaattaag cccagccatt atccatgatg tctgggtttt gtttactaac
     2701 gtcgtccccc taaaagcggc tcccggtaaa tataaatctt ctgttcatgt cgtaaatctt
     2761 gaggataacc aaatggtaga aagaaccgct gttttccctg ctggccgaca ttcactgtat
     2821 gctgagcatc gttattctgc ggctattcgt tccggcgatt tgctgtttgt ttccgggcaa
     2881 gtaggaagtc gagaggacgg aacaccagaa cccgattttc agcaacaagt cagactggca
     2941 tttgataatt tgcatgcgac cctggctgct gcgggatgca cttttgacga tatcattgat
     3001 gttacgagct ttcataccga tccagaaaaa caatttgaag acatcatgac ggtcaaaaat
     3061 gaaatattta gcgccccacc ttatccaaac tggacggcgg tgggtgttac ctggctggca
     3121 ggctttgatt ttgaaattaa agtgatagcg cgcatccctg agctataagc aatagtgtta
     3181 gccgttcgtt ttcacactcc gccctataag tcggatgaat ggaataaaat gcatatctga
     3241 ttgcgtgaaa gtgaaaaaga aaaaagcagg aaatgtctgc aattattgat accgaacgac
     3301 agttctcccc cagaatcaca tcaaataaaa atgcatgtat tttgactttt aattcaaata
     3361 aaccgtttgc gctgacaaaa tattgcatca aatgcttgca ccgcttctga cgatgagtat
     3421 aatgccggac aatttgccgg gaggatgtat ggttcagtgt gttcgacatt ttgtcttacc
     3481 gcgtctgaaa aaagacgctg gcctgccgtt tttcttcccg ttgatcaccc attcccagcc
     3541 cctcaatcga ggggcttttt tttgcccagg cgtcaggaga taaaagatgg ctaatccgct
     3601 atatcagaaa catatcattt ccataaacga ccttagtcgc gatgacctta atctggtgct
     3661 ggcgacagcg gcgaaactga aagcaaaccc gcaaccagag ctgttgaagc acaaagtcat
     3721 tgccagctgt ttcttcgaag cctctacccg tacccgcctc tctttcgaaa cttccatgca
     3781 ccgcctgggt gccagcgtgg tgggcttctc cgacagcgcc aatacatcac tgggtaaaaa
     3841 aggcgaaacg ctggccgata ccatttcggt tatcagcact tacgtcgatg cgatagtgat
     3901 gcgtcatccg caggaaggtg cggcgcgcct ggccaccgaa ttttctggca atgtaccggt
     3961 actgaatgcc ggtgatggct ccaaccaaca tccgacgcaa accttgctgg acttattcac
     4021 cattcaggaa acccaggggc gtctggacaa tctccacgtc gcaatggttg gtgacctgaa
     4081 atatggccgc accgttcact ccctgaccca ggcgctagcg aagttcgacg gcaaccgttt
     4141 ttactttatc gcgccggatg cgctggcaat gccgcaatac atccttgata tgctcgatga
     4201 aaaagggatc gcctggagtc tgcacagctc tattgaagaa gtgatggcgg aagtagacat
     4261 cctgtacatg acccgcgtgc aaaaagagcg tctggacccg tccgagtacg ccaacgtgaa
     4321 agcgcagttt gttcttcgcg ccagcgatct ccacaacgcc aaagccaata tgaaagtgct
     4381 gcatccgctg ccgcgtgttg atgagattgc gacggatgtg gataaaacgc ctcacgcctg
     4441 gtacttccag caagcaggca acgggatttt cgctcgccag gcgttactgg cactggttct
     4501 gaatcgcgat ctggtactgt aaggggaaat agagatgaca cacgataata aattgcaggt
     4561 tgaagctatt aaacgcggca cggtaattga ccatatcccc gcccagatcg gttttaagct
     4621 gttgagtctg ttcaagctga ccgaaacgga tcagcggatc accattggtc tgaacctgcc
     4681 ttctggcgag atgggccgca aagatctgat caaaatcgaa aatacctttt tgagtgaaga
     4741 tcaggtagat caactggcat tgtatgcacc gcaagccacg gttaaccgta tcgacaacta
     4801 tgaagtggtt ggcaaatcgc gccccagtct gccggagcgc atcgacaatg tgctggtctg
     4861 cccgaacagc aactgtatca gccatgccga accagtttct tccagctttg ccgtgcgaaa
     4921 acgcgccaat gacatcgcgc tcaaatgcaa atactgtgaa aaagagtttt cccataatgt
     4981 ggtgctggcc aattaattgc ggttggtaat aaaagtctgg ctccctataa tgagccagac
     5041 tttttaccgc tgtaataaag gagaaatcat gagcaaaact atcgcgacgg aaaatgcacc
     5101 ggcagctatc ggtccttacg tacagggcgt tgatctgggc aatatgatca tcacctccgg
     5161 tcagatcccg gtaaatccga aaacgggcga agtaccggca gacgtcgctg cacaggcacg
     5221 tcagtcgctg gataacgtaa aagcgatcgt cgaagccgct ggtctgaaag tgggcgacat
     5281 cgttaaaacc accgtgttcg tgaaagatct gaacgacttc gcaaccgtaa acgccactta
     5341 cgaagcgttc ttcaccgagc acaatgccac cttcccggca cgttcttgcg ttgaagttgc
     5401 ccgtctgccg aaagacgtga agattgagat cgaagcaatc gctgttcgtc gctaatcttg
     5461 atggaaatcc gggctatcat gcccggatta agtctgatga caaaagcaaa atcgcccgat
     5521 gcgctacgct tatcaggcct acgtgattcc tgcaatttat tgaatttgta ggccggattg
     5581 ggcgtttacg ccgcatccgg catgaacaaa gctcactttg tctgcaatct gaatccgggc
     5641 caccatgccc agtttattct ttactgccag ccgtaacgac ggctatagac ccctttcacc
     5701 aactgggtta acgtcatata ccctgccaga atcgcaacca gccacgggaa atagcttaac
     5761 ggcagcgcct gcagctgcag ataactggcc agcggtgaaa acggcaatgc gatcccgaca
     5821 atcatcacga tcacggtcat gatcattaac ggccacgatg cacagctctg aataaacggt
     5881 acacgacggg tacggatcat atgcacaatc agcgtttgcg acagtaagcc caccacaaac
     5941 cagcccgact ggaacagcgt ttgcgtttcc ggcgtgttgg catggaatac ccaccacatc
     6001 aggcaaaacg tcagaatatc gaagatcgag ctgatcggtc cgaagaagat cataaagcgc
     6061 cccagatccg ccggattcca acgctgcggc ttttgaattt gctcgtcgtc gacgttatca
     6121 aacgggatcg ccacctgtga cacatcgtac agcaagttct gaataagcaa gtgtaacggc
     6181 aacatcggca ggaagggcaa gaaagcactc gccaccagca cgctgaacac attaccgaag
     6241 ttagaactcg ccgtcatttt gatgtatttc agcatgttgg cgaaagtgcg acgtccctca
     6301 ataaccccct cttccagcac catcaggctt ttttccagca ggatgatatc agccgcttca
     6361 cgggcaatat ctaccgcgcc gtccacagaa atgccgatat ccgcagcgcg taaggcgggc
     6421 gcatcattaa taccatcgcc cataaagcca accacatgcc cttcgcgctt cagtaaggtc
     6481 acaatgcgtt ctttatgcat cggcgtcagg cgggcaaaca gcgtggtgcg ctgtgcgaga
     6541 tttgccagtt catcgtcaga tagcgtttca atatcactac caatgaccac ctctcccgca
     6601 tccagcccca cttcatggca cactttcgct gcaactaact cactgtcgcc agtaaggatt
     6661 tttacggtaa ttccactcgc ttttaatgcc ttcagtgccg gagcggttgt ctcttttggc
     6721 ggatcaagga aagcaatata tccttcgagg atcaggtcgg attcatccgc ccgctggtaa
     6781 tctccttcac gcgctggcag gtatttcgtc gccaccgcaa ccacgcgcag cccctgacga
     6841 ttcagcgtat cagtaacccg cttaatctta cgcagcatga tgtcatcgag cggcacaatc
     6901 tcgccattgt gacgcacctg cgaacacaca ttgaggattt cctgcaatgc acctttgcaa
     6961 accagctgat ggtgttcggt attttctgcc actaccaccg acatccgacg acgctcgaaa
     7021 tcaaacggaa tctcatcaat tttttgccaa cggctggcca gcgaacgcgc tgactcttca
     7081 tccgtacctt cgagcaccgc ggtatcaagc aggtttttaa gtccggtctg gtaatgactg
     7141 ttcaaccacg cgctatgcag cacgcgttcg ctggttttac cggagatatc ggtatgattc
     7201 tccagcacaa ttttgtcctg cgtcagggtg ccggttttat cagtgcacag aatatccatt
     7261 gcgccaaagt tctgaatggc atccagatgt ttgacgatca ctttctgttt cgacagcttt
     7321 actgcgccgc gcgccagcgt cgaggtaaca atcatcggca acatttctgg cgttaagcct
     7381 accgcgaccg aaagcgcaaa cagtgccgct tcccaccagt cgcctttggt gtaaccgttg
     7441 ataagcaaca ccaccggagc catcaccagc ataaagcgaa tcagcagcat actgacgcgg
     7501 ctaatccctt gctgaaaggc attcggctcg ctttcctgct cactaacacg ccccgccagt
     7561 tgaccaaacc aggtattggc ccctgtggca atcaccattg cttgtgccgt accgctcacc
     7621 acggtggtgc ccataaaaca cagggtgtcg cactccagcg gattgctgtg ctccggctgg
     7681 cgagtagtag cggctttttc cacgggcaga gactcaccgg ttaacgacgc ctgagcgacg
     7741 aacagatccc gcgcctgcaa gatacgtaaa tctgccggga tcatatcccc tgccgccagt
     7801 tttataatat cgccgggcac cagttggtcg atcgggatct ccagccagcc attttcgcct
     7861 ttgtcgttaa ttacgcgcag caccgtcgca gtattgctga ccatcgcttt cagggcatct
     7921 gccgctttag tggaacgtgc ttcctgaata aagttcagca acgtagaaat agcgaccatt
     7981 agcgcgataa cgcccgcggc aaataaatct tccgtggcgt aagaaatagc gccgagaatg
     8041 gtgagtaaaa tattaaaggg gttgcgatag cagacccata aatgaaccca ccacggcgac
     8101 ggttgttgtg cgggtaattt attttcacca tgttgttcgc gggcagattc cacttccgcc
     8161 tgatttaatc cttccggatg ggtgtcgaac gttttccaca attcttcttc gggcatcacc
     8221 gccattttca ggcaatgcgc acttaaggac ggcgggaccg ccgtattcac tgtctgctgc
     8281 gcgccaggca atggatcgcg atgaaccaga cgggaaggta aatggcgaat gaaccgggta
     8341 aaaatttctt taaacataag gagtccctcc gcactgtctg aaacagtaca gaaatgtctt
     8401 caggcgtggc gaagccttac ctgaaggtaa taaagctgag cagggacgtt aaaacgtcac
     8461 ggccttacgc atccggtaac gccgtgaaaa gacgggctta ccggaaaagt tttaatctcc
     8521 gtcgagggag aggcgtggga tcaggttcca tataacctcc ggtaagtgaa aaaagcgccg
     8581 caaataataa ggagaatcca ataatattcg tagcatttat ggcgatataa tacctgctgg
     8641 caactaaacc aacgataaac cagactttac ccattgctga atgcacaggt aaagttaggc
     8701 tcaaataatt aaacaacacg ttatgggaca acaggatgca aaatcggctg accatcaaag
     8761 acatcgcgcg cttaagcggc gtggggaaat ctacggtttc ccgggtgctg aataacgaaa
     8821 gcggcgtgag ccagcgcacg cgcgagcgtg ttgaagcagt gatgaatcag catggatttt
     8881 ccccttcccg ctctgcgcgc gctatgcgtg ggcaaagcga taaagtggtc gccatcattg
     8941 ttacccgtct ggattcgttg tcagaaaatc tcgccgttca aaccatgctg ccagcgttct
     9001 atgaacaagg ttacgatcca atcatgatgg aaagtcagtt ttccccacaa ttagttgccg
     9061 aacatttggg ggtgctgaaa cggcgtaata tcgacggcgt agtgctgttc ggttttaccg
     9121 gcataacaga agaaatgtta gcctactggc agtcatcgct ggttctgctg gcgcgtgacg
     9181 caaaaggctt tgcttcggtc tgttatgacg acgaaggggc aatcaagatc ctgatgcaac
     9241 ggctgtatga ccagggacat cgtaatatca gttatctcgg cgtgccgcac agtgacgtga
     9301 cgaccggtaa gcgacgtcac gaagcctacc tggcgttctg caaagcgcat aaattgcatc
     9361 ccgttgccgc tctgccaggg cttgctatga agcaaggcta tgagaacgta gcaaaagtga
     9421 ttacgcctga aactaccgcg ttactgtgcg caaccgacac gctggcactt ggcgcaagta
     9481 aatacctgca agagcaacgc atcgacacct tgcaactggc gagcgtcggt aatacgccgt
     9541 taatgaaatt cctccatccg gagatcgtca ccgtagatcc cggttacgcc gaagctggac
     9601 gccaggcggc ctgccagttg atcgcgcagg taaccgggcg cagcgaaccg caacaaatca
     9661 tcattcccgc caccctgtcc tgatcgtttc ctgaacgata aattgtgatc ttcgctgcgt
     9721 ttcgggaacg ttcccgtttt taaatttttc agcgcaatat attctgcagc caaccaaaaa
     9781 tgtcatctgc catggggctt tatgatgagc aaaataaacc aaacggatat cgatcggttg
     9841 attgaactgg tcggcgggcg cggcaatatt gcgacggtga gccactgtat tactcgtctg
     9901 cgctttgtcc tcaaccaacc ggccaatgcc agaccgaaag aaattgagca actccccatg
     9961 gtgaaaggct gtttcaccaa tgccgggcaa tttcaggtgg tgattggcac caacgtgggt
    10021 gattactatc aagcactgat agcgtcaacc ggacaggcgc aggttgataa agagcaggta
    10081 aaaaaagccg cccggcagaa tatgaaatgg catgagcagt tgatctctca tttcgcggag
    10141 attttcttcc cgttgctgcc cgcgttgatt agcggcggtt tgatcctcgg ttttcgcaat
    10201 gtgatcggcg atttgcccat gagcaacggt caaacgctgg cgcaaatgta cccttccctg
    10261 caaacgatct acgattttct gtggttgatc ggtgaagcga tcttcttcta cctgccggtc
    10321 gggatttgct ggtcagcggt gaaaaaaatg ggcggcacgc cgatccttgg tatcgtgctt
    10381 ggcgtaacgc tggtttctcc acagctgatg aacgcttatc tgctcgggca gcagctgccg
    10441 gaagtgtggg actttggcat gttcagcatc gccaaagtgg gctatcaggc gcaggtgatc
    10501 ccggcactgt tagccgggct ggcgctgggc gttattgaaa ctcgtcttaa gcgcatcgtg
    10561 ccggattacc tctatctggt ggtggtgccc gtttgttcgc tgatcctcgc ggtgttcctc
    10621 gcccatgcgc tgattggtcc gtttggtcgc atgattggcg atggcgttgc ctttgcggta
    10681 cgtcacctga tgaccggcag ctttgctccg attggcgcgg cattgtttgg cttcctgtac
    10741 gccccgctgg tgatcaccgg cgtacatcag accacgcttg ctattgattt gcagatgatt
    10801 caaagcatgg gtggtacgcc agtgtggccg ctgattgcgc tgtcgaatat cgctcagggt
    10861 tccgccgtga taggcattat catttccagc cgcaagcaca atgaacgcga gatctccgtg
    10921 cctgccgcta tctccgcctg gcttggagtc actgagcctg ctatgtacgg catcaacctg
    10981 aaatatcgct tcccgatgct gtgcgcgatg attggttctg gtctggcagg attgctatgc
    11041 ggcctgaacg gcgttatggc gaatggcatc ggcgtaggcg gcctgccggg aattctctcg
    11101 attcaaccga gctactggca ggtgtttgcg ctggcaatgg ctatcgccat catcatcccg
    11161 attgtactca cctcgtttat ctatcagcgg aaataccgcc tgggcacgct ggacattgtt
    11221 taattttctt cggggcgcaa ttgcgccccc tcgcatttgc aggaataacg taatgactaa
    11281 tcttccccac tggtggcaaa acggcgttat ctaccagatt tatccaaaga gttttcagga
    11341 caccacgggt agcggcaccg gcgatttacg tggcgttatc cagcgcctgg actatctgca
    11401 taaactgggc gttgatgcca tctggctaac ccccttttat gtctctcccc aggttgataa
    11461 cggttacgac gtagcgaact atacggctat tgatcccacc tacggcacgc tggacgattt
    11521 tgacgaactg gtgacacagg caaaatcgcg cgggattcgt atcattctcg atatggtgtt
    11581 taaccatacc tctacccaac atgcctggtt tcgcgaggcg ctgaacaaag caagccctta
    11641 ccgccagttt tatatctggc gcgatggaga atcagaaacg ccaccgaaca actggcgttc
    11701 aaaatttggc ggtagtgcgt ggcgctggca tgcggaaagc gaacagtact atttgcatct
    11761 ctttgcacca gaacaggcgg atctcaactg ggagaatcca gcggtacgcg cagagctgaa
    11821 aaaagtctgt gagttctggg ccgatcgtgg ggtcgacggg ttgcgcctgg atgtggtgaa
    11881 tctgatctcc aaagacccgc gtttccctga tgacctggat ggcgacgggc gtcgcttcta
    11941 caccgacggg ccacgagcac acgagttttt gcacgagatg aaccgcgatg tgtttacgcc
    12001 acgcaggtta atgaccgtag gtgaaatgtc ctccaccagc cttgagcatt gccagcgata
    12061 tgcgacactg acaggcagtg aattgtcgat gacctttaat tttcatcacc tgaaagtcga
    12121 ttatcccagt ggtgaaaaat ggacgctggc taaacctgac tttgtggcgt tgaaaacatt
    12181 gttccgccac tggcaacaag gaatgcacaa cgtggcatgg aatgccttgt tctggtgtaa
    12241 ccacgatcag ccgcgcattg tttctcgctt tggtgatgaa ggtgaatacc gcgtgcctgc
    12301 ggcaaaaatg ctggcgatgg tgctgcatgg catgcaggga acgccgtata tctaccaggg
    12361 cgaagagatt ggcatgacca acccgcattt cacgcgcatt actgactatc gcgacgtgga
    12421 gagcctcaat atgtttgccg agctgcgcaa cgatggtcgt gatgccgacg agttattggc
    12481 aatcctcgcc agtaaatccc gtgacaacag tcgcacaccc atgcaatgga gcaacggcga
    12541 taatgccggg tttacggctg gcgaaccgtg gattggcctg ggcgataact atcaggaaat
    12601 caacgtagaa gcagcgctgg ccgatgagtc ctcggtgttt tacacctacc aaaagttaat
    12661 cgcactgcgt aagcaagaag ccgtcctgac atggggcgat taccaggatc tgctgccaaa
    12721 cagccctgta ttgtggtgct atcgccgtga gtggaagggg caaaccttac tggtcattgc
    12781 caaccttagc cgtgagaccc aaccctggca gccagggaaa atgctcggca actggcagct
    12841 tgtgatgcat aactacgaag aagcctcacc acaaccctgt gccatgactt tacggccttt
    12901 tgaggctgtc tggtggttac agaagtaagt atccgctaag ccccggtaat actggtcatt
    12961 ccggggtttt gcctttcagc atgttgattt tataatcaaa aaattcatta aaaaatttac
    13021 ataacgctgt agcgcccgtc atccgtacgc tctgcttttt actttgagct acatcaaaaa
    13081 aagttcaaac atccttgatg caaagcacta tatatagact ttaaaatgcg tcccgaccca
    13141 atatgttgta ttaatcgact ataattgcta ctacaactcc ccacgaaaaa ggtgcggcgt
    13201 tgtggataag cggatggcga ttgcggaaag cgccggaaaa cgaaacgaaa aaaccggaaa
    13261 acacctttcc caatttctgt ggataacctg ttcttaaaaa tatggagcga tcatgacacc
    13321 gcatgtgatg aaacgagacg gctgcaaagt gccgtttaaa tcagagcgca tcaaagaagc
    13381 gattctgcgt gcagctaaag cagcggaagt cgatgatgca gattattgcg ccactgttgc
    13441 cgcggttgtc agcgagcaga tgcagggccg cagccaggtg gatatcaacg agatccagac
    13501 cgccgttgaa aaccagctta tgtcgggtcc atacaaacaa ctggctcgtg cttacatcga
    13561 gtaccgtcac gatcgcgaca ttgaacgtga aaaacgcggt cgcctgaatc aggagatccg
    13621 tggtctggtt gagcagacca acgcctcgtt gctcaacgaa aacgccaaca aagacagcaa
    13681 ggtgattcca acccagcgcg acctgctggc cgggattgtg gctaaacact atgcacgcca
    13741 gcacctgctg ccgcgtgacg tggtgcaggc acatgaacgt ggtgatattc actatcacga
    13801 tctcgattac tcaccgttct tcccgatgtt caactgcatg ttgatcgacc tgaaaggcat
    13861 gctgacccag ggctttaaaa tggggaacgc cgagattgaa ccgccgaagt cgatctctac
    13921 ggcaaccgcg gtaactgcgc agattattgc ccaggtcgcc agccatattt atggcggcac
    13981 caccattaac cgtatcgatg aagtgctggc accgtttgtc accgccagct acaacaaaca
    14041 tcgcaagacc gcagaagagt ggaacatccc ggacgccgaa ggctacgcta actctcgtac
    14101 catcaaagag tgctacgatg ccttccagtc gctggagtac gaagtaaaca ccctgcacac
    14161 cgccaacggt caaacgccgt ttgtaacctt tggttttggt ctgggcacca gctgggagtc
    14221 gcgcctgatt caggaatcta tcctgcgtaa ccgtatcgcc ggcctcggta aaaaccgtaa
    14281 aacggcggtg ttcccgaaac tggtgtttgc gattcgcgat ggcctgaacc ataaaaaagg
    14341 cgatccgaac tacgacatca aacagctggc gctggagtgc gcaagcaagc gcatgtatcc
    14401 ggatatcctg aactacgatc aggtagtgaa agtcaccggt tcgtttaaaa ccccgatggg
    14461 ctgccgcagc ttcctcggcg tgtgggaaaa tgaaaacggc gagcagatcc acgatggtcg
    14521 taacaacctc ggcgtgatca gcctgaacct gccgcgtatt gctctggagg caaaaggcga
    14581 tgaagccacc ttctggaagc tgctggatga acgtctggtg ctggcacgta aggcgttgat
    14641 gacccgtatc gctcgtctcg aaggcgtgaa agcgcgcgtg gccccgatcc tctatatgga
    14701 aggtgcttgt ggcgtgcgtc tgaatgctga cgacgacgtt tctgaaatct tcaaaaacgg
    14761 tcgtgcgtct atttcgctgg gttacatcgg catccacgaa accattaacg cgctgttcgg
    14821 cggcgagcat gtttacgaca acgagcagct gcgcgcgaaa ggtattgcga ttgttgagcg
    14881 tctgcgtcag gcggtggatc agtggaaaga agaaacgggt tatggtttca gtctctacag
    14941 cacgccaagt gaaaacctgt gcgaccgctt ctgccgtctc gataccgctg agtttggcgt
    15001 ggtgccgggc gtgaccgata aaggttacta caccaacagt ttccaccttg atgtggagaa
    15061 aaaggtgaac ccgtatgaca agatcgactt tgaagcgcct tacccgccgc tggcgaacgg
    15121 tggtttcatt tgctacggcg agtatccaaa cattcagcac aacctgaagg cgctggaaga
    15181 tgtctgggat tacagctatc agcatgtacc gtattacggc accaatacgc cgatcgatga
    15241 gtgctacgag tgtggcttta ccggtgagtt cgagtgcacc agcaaaggct tcacttgtcc
    15301 gaaatgtggt aaccatgacg cctcccgtgt gtcggtgact cgccgcgtgt gcggatattt
    15361 aggtagcccg gatgcacgtc catttaacgc cggtaagcag gaagaagtta agcgccgcgt
    15421 taaacatttg gggaatgggc agataggtta atcttcactc tgcgttaagc aaatgccgga
    15481 tgcggcgtaa acgccttatc cggcctacat ttggcaacgg ctgtaggcct gataagacgc
    15541 gacagcgtcg catcaggcgt tgattactgg atgcagccta cacgcggact gataagccag
    15601 gcgaaataat gaattatcat cagtactatc ctgtcgacat cgtcaacggc cccggcactc
    15661 gctgcaccct gtttgtctcc gggtgtgttc atgaatgccc cggttgctat aacaaaagca
    15721 cctggcgggt aaattccggt cagccattta ccaaagcaat ggaagaccag atcattaacg
    15781 atctgaatga cactcgcatc aaacgccagg gtatttccct ctccggcggc gatccgctgc
    15841 atccgcaaaa cgtgccggat attctgaaac tggtacaacg catccgcgcc gaatgtccgg
    15901 gtaaagacat ctgggtgtgg acaggctata aactcgacga actcaacgct gcgcaaatgc
    15961 aggttgttga tttgattaac gtgctggtcg acggcaaatt tgtgcaggat ttaaaagacc
    16021 cttccctgat ctggcgcggc agcagcaatc aggtggtgca tcatttgcga tgataagttc
    16081 cctgttacct gagtgcaggc aacagggaaa taaggaatta acgatacttc tggtgatagg
    16141 cgttgcgggt cgttttcagt tgctctgcgg cagcctgcgc ttcttttact ttaccttcat
    16201 ttgccagctt cagcgcgtcg tcaatctgac cgaccagaat atcgaaaccg tggcggaagt
    16261 ctttcatttc cgggctgtcc ggtgatttgc cttcgagctt cggcggcgtc gctttttgcg
    16321 catccagcgc tgcggcgcgc atcttcgtta acgcgtcttt gacttgcgcc gcgttatcgg
    16381 ctttttcgac cacttttaaa ttgtcgttga gggtttccat attgtcttca agatcagcgg
    16441 caaacgacgc cgaactgaat accaacgagg agactgcaag aatagctaac aggcttttac
    16501 gcattgctca cttccttttt tatttacact tcatccttca cactgcctca tcgacggcta
    16561 cgctcattcg cccgagtcac gtactgcgag tacgctcccc ggtcctcatt cactttccgt
    16621 ctcgatgcag tgcgaatgat tttgtgtaat tttttagttt atagcgcggc aggtcgcgcc
    16681 agttttttac tgcccggcga ttttcatctc cggcaacagc acagaaccac actgtatatt
    16741 actgcgtgtt tcaatatcgt taccgacggt gacaatattg cgccacatat cttttaaatt
    16801 acctgcgatg gtgatttcgc taaccggata ctgaatttcg ccattctcta cccagaagcc
    16861 cgctgcacca cgggaataat caccggtaat ggcactcacg ccctggccca tcagctccgt
    16921 caccaccagc ccggtgccca tctctttgag catttgttcg aagcttagcc cttgtccggc
    16981 aatccgccag ttgtgaatac cgcccgcatg tccggtgctt ttcagcccca gtttccgcgc
    17041 cgaatagctg gtcagcagcc actgagtcag gatgccatct ttaataatat cgcgacgctc
    17101 ggtgcgcaca ccttcgctgt cgaatggcgt agaggccagc cctttcagca gatgcggatg
    17161 ctcttcaatg gtcagccagt ccggcagaat ttgtttaccc agcgaatcca gcaggaaggt
    17221 agatttacgg taaaccgatc cacccgctat cgccccaacc agatgcccaa aaagcccggt
    17281 tgccacttca ttggcaaaaa tgaccggcgc tttcatggtg gagagtttac gcggtgacag
    17341 acgcgataaa gtgcggcgag cacagtcggc cccgacccac tctggcgttt gcagatcgct
    17401 catcgcacga ccaatggtgt aggcgtaatc acgctccata tcgccatttt cttcggcaat
    17461 tacacagctg gagagcgaat gacgcgtaga gcagtaaccc tgcaacatgc cgtggctgtt
    17521 gccaaaaact ttgacaccgt agtggctgtt aaagctgcca ccttcggtat tggtgatgcg
    17581 tttgtccgcc tgcaatgctg cctgttctgc gcgggccgcc agttcaatgg cttcatccgg
    17641 ggaaacttcc gcagggtgga acaagtcgag atccggtgcg tcaaaggcca gcagctcttt
    17701 gtctgccacg ccggcacagg gatctggcga ggtataacgg gcaatatcta gcgccgcctg
    17761 tacggtgcgg gcaatggcct gcgggcttaa atcggtagac gatgcgctac ctttgcggtt
    17821 ctggtgataa acagtgatcc ccaacgcgcc atcgctattg aattcaacat tctccacttc
    17881 accataacgc gtgcttacgc taatgccggt ggtcttgctg acggcaactt ctgcaccgtc
    17941 cgatttgcct gaggccaact ccagcgcagt cgaaactgct tcttccagaa tcttgcgctg
    18001 cgcttcaact tgagagatta ctttcattgc aagtgccata atgtagagag tttctctgaa
    18061 gtctaacaga gaaccgtttt tcagtgcgca tcttaactgg taacattagc ctcttttttt
    18121 aaggagcctg agatgactaa gcagcccgaa gactggctcg acgacgttcc cggtgatgac
    18181 atcgaagacg aagacgatga aattatctgg gtcagtaaaa gtgaaattaa acgcgatgcc
    18241 gaggagctaa aacgccttgg cgcggaaatt gttgatctgg ggaaaaacgc gctggataag
    18301 atcccgttag atgcggatct gcgcgcggct attgagctcg cccagcgtat taagatggaa
    18361 ggtcgccgcc gccagttgca gctcatcggt aagatgctgc gccagcgcga tgttgagcct
    18421 attcgtcagg cgctggataa gctgaaaaac cgccacaacc agcaagtggt gttgttccat
    18481 aaactggaaa acctgcgcga tcgtctgatc gagcaaggtg atgacgccat cgctgaagta
    18541 ttgaatctgt ggcccgatgc cgatcgtcag caactgcgta ctctgatccg taacgcgaag
    18601 aaagagaaag aagggaataa gccgccgaaa tccgcgcgcc agattttcca gtatctgcgt
    18661 gagttagcgg aaaacgaagg gtaattttcg ttgttgatgt gagtatggtt tgccggatgc
    18721 ggtgcacctg taggcctgat aagacgcatc aagcgtcgca tcaggcacta tgctcaaatg
    18781 ccggatgcgg cgtgaacgcc ttatccggcc tacacacact ctatcaggct gaggctgtga
    18841 attactgcgc ggcttccgct ttcttcgcta acccatccag cagtttctga tggatcccac
    18901 caaaaccgcc gttactcatc accagaatgt gatcgcccgg ctgagcggtt ttcaccacca
    18961 tatctgccag cgtatccaca tcgccgctcc agtgtgcagg ctgaacgcag gcttctgcca
    19021 cttctgccac ctgccacgga atatgcgccg gttgcagcag gaagacttca tcggcacgac
    19081 ctaatgaagg tgccagatcg tctttgcaga tccccatttt catggtgttt gagcgcggtt
    19141 ccagcacggc aataatgcgc gccgtaccac caactttgcc acgcagcgcc gcaagcgttg
    19201 ccagaatcgc cgtcgggtga tgggcaaaat cgtcatatac cgtcacacca ttcgcttcac
    19261 cacgcaactc cagacggcga cgagcattaa taaacgaacc cagcgcgtta gcggcatctg
    19321 ccggcgctac accaacatgg cgagccgccg caatcgccat caggccattg tgcatattat
    19381 gttcgcctac cagcgaccat ttcacttcgc ccactttttc gccatccagc aagacttccc
    19441 attcggaagc atcggtggtc agctttttcg cctgccagtg accttgctcg cccaccagct
    19501 cctgctcgct ccagcagccc atcgccatgg tctgtttcag gttgatgtca ttttccggcc
    19561 agataatacg gccctgcccc ggaacgatac gcaccagatg gtggaactgt ttctggatcg
    19621 ctttcaggtc gtcaaagata tcggcgtgat cgaactcaag gttgttgagg atcagcgtac
    19681 gcgggcagta atggacaaat ttagagcgtt tgtcgaagaa ggcgcagtca tactcatccg
    19741 cttcgataac aaagaagtcg ctttcgccca gacgcgccga aacctcaaag ttccccggca
    19801 caccgccgat cacaaatccc ggtttgtaac cgcactgttc cagaatccag gttgccattc
    19861 ccgcggtggt ggttttgcca tgtgtaccgg caacggccag cacccagcgg tcgcgcagca
    19921 caaaatcgtg cagccactgc ggacctgaca tataagggat gtttttttcc agtaccgctt
    19981 ccacacacgg atttccacgg gtcatggcgt taccaataat caccagatcc ggctgtggat
    20041 cgagctggct ggcatcgtaa ccctgaatca gttcaatgcc ttgcttctca agtaaggtgc
    20101 tcatcggcgg atacacattg gcgtccgaac ccgttacttc atggcctaac tggcgcgcca
    20161 gcatcgccag accgcccata aacgtgccac aaattcctaa aatatgaatg cgcatacgtc
    20221 actatccttt tttaatctgg cgctcatttt acgcatatgt cgggcaagtg agaaacgcat
    20281 ttcaggataa tccgtaattt gctggcgcga ttcacctgag cgcaacattg ggattatttg
    20341 ttaagattgt tgcggtcgct ttactccata aacattgcag ggaaagtttt atgaaaacgt
    20401 taggtgaatt tattgtcgaa aagcagcacg agttttctca tgctaccggt gagctcactg
    20461 ctttgctgtc ggcaataaaa ctgggcgcca agattatcca tcgcgatatc aacaaagcag
    20521 gactggttga tatcctgggt gccagcggtg ctgagaacgt gcagggcgag gttcagcaga
    20581 aactcgacct gttcgctaat gagaaactga aagccgcact gaaagcacgc gatatcgttg
    20641 cgggcattgc ctctgaagaa gaagatgaga ttgtcgtctt tgaaggctgt gaacacgcaa
    20701 aatacgtggt gctgatggac ccactggatg gctcgtccaa catcgatgtt aacgtctctg
    20761 tcggtaccat tttctccatc taccgccgcg ttacgcctgt tggcacgccg gtaacggaag
    20821 aagatttcct ccagcctggt aacaaacagg ttgcggcagg ttacgtggta tacggctcct
    20881 ctactatgct ggtttacacc accggatgcg gtgttcacgc ctttacttat gatccttcgc
    20941 tgggtgtttt ctgcctgtgc caggaacgga tgcgcttccc ggagaaaggc aaaacctact
    21001 ccatcaacga aggaaactac attaagtttc cgaacggggt gaagaagtac attaaattct
    21061 gccaggaaga agataaatcc accaaccgcc cgtatacctc acgttatatc ggttcactgg
    21121 tcgcggattt ccaccgtaac ctgctgaaag gcggtattta tctctaccca agcaccgcca
    21181 gccacccgga cggtaaactg cgtttactgt atgagtgcaa cccgatggca ttcctggcgg
    21241 aacaagcagg cggtaaagcg agcgatggca aagagcgtat tctggatatc atcccggaaa
    21301 ccctgcacca gcgccgttca ttctttgtcg gcaacgacca tatggttgaa gatgtcgaac
    21361 gctttatccg tgagttcccg gacgcgtaat ctcctgcgcc tctccacacg gagaggcgtt
    21421 tttatcgctg cgcaatgttg actcttgtca caggcgaact ctgacgattc tcccacagca
    21481 ccgttaatcc acgctgtaat gctataaaaa taaacaacaa aataccgatg gcgattttcg
    21541 tccaccatga gctcagcgtg ccatcaaagt ttatgtaagt ctgaatcagt ccctgaatcg
    21601 ccacgccaaa aagcgtccct aataccgttc caacgccacc actcaaaagc gtaccgccaa
    21661 ttaccactga ggcgatagca tccagttcca cacctacgcc cgccagcgca tatccggcct
    21721 gggtataaat cgagaagaca atccccgcca gtgtcgccag cccggtagag agcatataaa
    21781 tgcgaatagt ggtgctgcga gtggaaatcc ccatcaggtt cgccgacgtt gcgttgccgc
    21841 caatggcgta cacctgatta ccaaaacgcg tacgatgcgc gaggaatatg ccgataacca
    21901 ccaccgccag catcagcagc cccatcgcac ttaagcgacc gccgccaggg attttccacg
    21961 caaggcttga gagcgtgtca taaacaggat ggtttatcgg gatcgactct tccgaaacga
    22021 gatagctgac gccacgcaaa aagaacattc ctgccaaagt aatgataaac gccgggatct
    22081 tcagggcgtc aatcagaagc cccataaatg cgccgaaggc acagcccatc accaacacca
    22141 gcggaaacgc cagcagcggc gagaggccga aatcgccaat cacttttgcc agaaacacgc
    22201 cggtaaaggc gatcaccgaa ccgacggaga gatcgatccc accggagagg atcacaaagg
    22261 tcatgccaac ggcgattatc ccaagaaagg cattatcggt caggatattg cagatcactc
    22321 ttgtggaagc aaaaccggga aactgggtca gacagtagat ataacccaac acaaagacgc
    22381 cgatggtgat catcagtggc aaattacgtt ttatcacggt tacgtacccc tttaatcaga
    22441 ctgataaagc gttgcgactg gacaatcagc acgcaaagca ccaccaccgc tttcaccacc
    22501 tggttcatct ccggcggaaa tccagaaagc aaaattccgg tgttcattcc ctgaataatc
    22561 agcgccccca ccaccgaaag cagcaggtta aagcgcccgc ccatcagcga tccgccgcca
    22621 atcaccaccg cgagaatggc gtccagctcc agccataacc cggcgttatt ggcatcggca
    22681 ccgcgaatat ccgccgccac gataatgccc gcaatcgccg cacatagccc gctcaacacg
    22741 taggtgagca tgacgatgat tcgcgtgttt accccggcat tttttgccgc ccgaatattg
    22801 ataccaacgg cttcgataaa catccccagt gccgttttgc gggtcaacag ccagaacagg
    22861 agaagcgtca gcaccgcaat aatgactggc gttggcagga acaacagcga tccactgccg
    22921 aaccaggaga gatccggtga gttaaatgtg acgatctgtc cggaggtgat cagttgcgcc
    22981 acaccgcgcc cggcgaccat caggatcagg gtggcgacaa acggctgaat tttgaggatc
    23041 gctaccagta tgccgttcca caatcctgcc aggatgccag tgcccagggc gcttaacaaa
    23101 acaatcggca ggctgaatcc cgcgaccgtc atcgcagccg ttgtggctcc ggcgatagcc
    23161 atcaccgccc ctacggagag atcaatccca ccggtggcga taaccagcgt catgccaatc
    23221 gccagtagcg caacgggggc cgcacggtta agaatgtcga tagggctacc gaacaaacgc
    23281 ccatcctgaa gcaccacctg ccagaaatgc ggggccacca ggctatcgac cagcagcacc
    23341 agcaacagtg ccgccagctg cggcattcct gttggccagc gaaaacgcct tttcggcggc
    23401 gtagtgtccg ggagagattg aggcatcaca ctattctcct tacgccgcaa tggcgttcat
    23461 gatcgccgga acggaaagct ctgccagcgg gatctccgcc acctgtttgc gatcgcgcat
    23521 gataatcacc cggtcggcat agcccaccag ctcttccagt tcagaggaga tcaccagcag
    23581 cgccagaccg tcagcgcaca gcgtttcaat caggcggatg atttcagcgt gggcaccaac
    23641 atcaatgccg cgggttggct cgtcgaggat caaaaattgc ggacgagtca gtagccaacg
    23701 tgaaagcaac actttttgct gattgccgcc agagagaaat tcaatcggtt gttcagttga
    23761 aggtgtgcga atgccaagct ggcggataaa gcgttcggca atctcttgct gttctttgcg
    23821 ggaaatcggt cgtagccagc cgcgctgggc ctggagagcg aggatgatat tttcccgcac
    23881 cgaggcggcg gcgatgatgc catcggtttt cctgtcttcc gggcagaagc cgatacccag
    23941 taccgaagcc tgatgtggcg atcgcagggt ttgctgtttg cctttgatca acgccgtgcc
    24001 gctgtcagca ggtttgatcc cgaagatcac ttcggcggtt tcggtacgtc cggatcccag
    24061 caagcctgcc aggccaacga tctcgccggg gcgtacttcg agatcaaacg gtgcgatcgt
    24121 tccttttttg ccgtaatttt tgaacgcggc aacgggtttg tcgctcagca atgttctccc
    24181 ggcacgctga agcgcgtggg tgtccagttc acgccccagc atcattttta ccagttcgat
    24241 ctgcggtagc tcgcgggttt cccgacagcc cacaaaactg ccgttacgta agacggtgat
    24301 ccgatcgcta acctgataaa cctgatcgag aaaatgagtg acgaagatca ggctgacgcc
    24361 gcgatcgcga agctgacgca tcaggccaaa cagtaactct acttcctgag tgtcgagact
    24421 ggcggtgggt tcatcgagga tcagcacttt ggcggagaga tcgatagccc ggcaaatggc
    24481 gacgatttgc tgcatcgcga ctgaaaagcg gttgagcggt tcgcgcacgt cgagggagaa
    24541 accgtaagat gccatcagtt cggtggcgcg cttttccatc tctttacggc gtagaaggcc
    24601 gaaacgtttg ggttcgcgac ctatgaatag attatcagcg accgacatat tgggtagcag
    24661 gttgacttcc tgatagacgg tgccgatgcc gagttgttgt gcgtgggcgg tgtttttcgg
    24721 tgagatagcc tggccttcca gccagatagt gccgcgatcg gcgtggtata caccggttaa
    24781 tgctttgatt agcgtcgatt ttcccgcccc gttttcaccg agcagcgcca tgatttcgcc
    24841 acggcgcagg ctgaaatcaa cgttgtctaa cgctttgaca ccggggaaaa atttacttaa
    24901 tccttcggtg cggaggattt cctggtgttg gtcggtcgtc atggtttccc ccggtgtaaa
    24961 gcgtgctccc agtaggcatg ataagacgcg gtaagcgtcg catcaggctt cagcgcacaa
    25021 ccgccggatg cggcgcgagc gccttatccg gcctacgaat cggcatcagg catagcaacc
    25081 ctcaataccc catatttttc ttcttctcta actcttcttt tgcggtatca ggcagataaa
    25141 gggtggattt ggttaacgtc agcttttcag gcatggtgcc gtctttttta tatttctcca
    25201 gcgcgtcgaa ggcgggacct gccatattcg gcgtcagttc aacactggcg ttcgcttcgc
    25261 catcaatcat cgctttgtag atgtccggca caccgtcgat agagcctgtc aggatatctt
    25321 tgcccggttt caggcccgct tctttaattg cctgaattgc accaatcacc atgtcatcgt
    25381 tatgggcgta aaccatgcag atgtttttgc cgttgttttc cgctttgata aagctctcca
    25441 tcacttcttt gcctttgctg cgggtgaagt cacctgactg cgagcggata attttgatat
    25501 ttggcgcatt cttaatggct tcggcaaagc ctttcttacg gtcaatggcg acgctggccc
    25561 caacggtgcc ctgcagctcc accacgttgc atggtttgcc attcacttct tttaccagcc
    25621 agtcaccaat caacttgcct tcgaggatgt tgtcggcggt gacagtggtc atatagagag
    25681 atttgtcttt cacatcgatg gaacggtcaa gcaagaagac cgggatttcg gcatctttcg
    25741 cctcttttaa taccggctcc caaccggtcg ctaccactgg agcgataaag atcgcatcca
    25801 ccccttgcgc gacgaaggaa cgtaccgctt taatctggtt ttcctgcttt tgctgaccat
    25861 cggcaatttt caacgtaatt ccgcgctttt cggcttcact tttcgccaca ttggtttctg
    25921 cggcgcgcca gccggattcc gacccgacct gcgaaaatcc aacggttaat ggagcggcca
    25981 acgccataga cgacatggct gccgagactg cagagactac aagtaagcgt ttccacatag
    26041 ggttgtcctc gtcggggtaa ttattattgg tgaaaagatg ttcgcgaaaa aactatagac
    26101 aattcgttat gtaacggatt gcgttacatc acacttcaaa aaagtaaata aaacgttaat
    26161 cacaagtttg taatcgcttt catctcacta tgaaaaatgc ggctacggtt atggattttc
    26221 ctgctctgta taccgtctta aaactggcga aaaaggaaaa tgaagacgaa aacaagcgaa
    26281 gacattcggc gcgagttggc tataatactc ggcacttgtt tgccacatat ttttaaagga
    26341 aacagacatg agcttactca acgtccctgc gggtaaagat ctgccggaag acatctacgt
    26401 tgttattgag atcccggcta acgcagatcc aatcaaatac gaaatcgaca aagagagcgg
    26461 cgcactgttc gttgaccgct tcatgtccac cgcgatgttc tatccgtgca actacggtta
    26521 catcaaccac actctgtctc tggacggtga cccggttgac gtactggtcc cgactccgta
    26581 cccgctgcag ccgggttctg tgatccgctg ccgtccggtt ggcgtactga aaatgaccga
    26641 cgaagccggt gaagatgcga aactgattgc tgttccgcac accaagctga gcaaagaata
    26701 cgatcacatt aaagacgtta acgatctgcc agagctgctg aaagcgcaaa tcgctcactt
    26761 cttcgagcac tacaaagacc tcgaaaaagg caagtgggtt aaagttgaag gttgggaaaa
    26821 cgcagaagcc gctaaagctg aaatcgttgc ttccttcgag cgcgccaaga ataaataagt
    26881 tcttctggcg caataaccct gaacaccggg cttcggttag taagggtttt tttatgcccg
    26941 caatttataa actctctact ccactattat tattttcagc agttgaaagg cttgaacggt
    27001 aagaacaagc taaactcgac caccattttg ctgttcatag ccacttgctg gaagttagcc
    27061 ggcctcactc atactcaccg ttcaaagcgt gtccccgttt tacccctgta catttgttaa
    27121 cgccagcaga gttttttatg cgattgcaga tatggaaagc ggcccggaga gaaaagcaaa
    27181 agatgggaaa gttacaaaaa aaatcgcgag atacatcgca taaaacaacg ccaccttttc
    27241 ggtggcgttt ttatcgccca cgcaccacca gtgcctggtc cagctcgcga tacgcttcaa
    27301 ccagtttctc cagtgaaacg cgacttaaac cgctgggatt tggcagcacc caaatctgcg
    27361 tcgaaccaat ggtgagagtt tgtttccccc actgtgcacc gcgctggctg aatccctgtt
    27421 catatgcttg tttgcccaga atcgccaacg cctgcggctg ataatcttca attttttcaa
    27481 tcagcttacg cccgcctgcg tgtagctcct gctttgaaac ttcattggct tgcaccgttg
    27541 gacggtctac cagtttggtg acgccacaac gataatccag cagatgctgt gcctcctgcg
    27601 gcttcaactg acggtcggta aacccggcct gatatatcac cttccagaag cgatttgccg
    27661 gatgagcaaa gggaaaacca gtcccggcgg atgaaagccc agggttgata ccgcaaaaca
    27721 cgacccgtaa ccctggagcc aaaatatcct caaccatatt tactcctttc taacatcacc
    27781 aggaaaagta taacggattg aaaatacatt gtttataaaa acagcaggcg cgcggtaatg
    27841 gctggattgc gacacggagt tactttataa tccgctacca tggcccctta gctcagtggt
    27901 tagagcaggc gactcataat cgcttggtcg ttggttcaaa cccaacaggg gccaccatat
    27961 acaacaaagg gttagctagt aaatcagcta accctttttc ttttgccttt gaattgggtc
    28021 aggtaatggg tcaggtaagc tttttctcca tctgactcag atggctgata gttgccccgg
    28081 ataatgcttc gcaatgatgt catttagccg atcaaccagt tctggttttc tgaacgtgat
    28141 atgcgcggag cccttcttaa agtatctgat actgaacatc tcatcttcat agtcctcttt
    28201 accctgaaca gactggatgt gatcatccag atgaatggtg atgttttccc ggttgtcggg
    28261 gatcggtttg ccgctgaaca ggtgtagcat tctttccaga tcggcaagtc gatcggcctg
    28321 ttgcccgctg ttcagatgaa atccccacct gtcccacctc accagattgt tgacgataat
    28381 tttactgcca aatttacagg gtgagttggt tttgtaatcc cagctcagtc ccctgaagac
    28441 gttgatcacc cctcgctcaa acacctcatt cttattctgg tgcagttgtt caaatgtgct
    28501 cagaatgttc gcttcactga tttccggaaa attatcgtac tccagtgacc ggtaccatgt
    28561 atcacgggcc tgtgcatcca ttaaggaaag catccctgaa cgttgcatca ggtcacgcca
    28621 gatttctcga tcaagattgc gggtgatggc tttcattgcc gtttcaggtt tttccatcag
    28681 ccagcaaccg cagcgaaaat cctgtttcat tgcccagtcc agtgcggttt taccgccaat
    28741 actttctgtc attgctgata tttcagccag ttgccggata agtgtgtcga tttgcagcag
    28801 tgtactcttt cgtacagcca gaatgtggcg gatactggtt
//
LOCUS       DAZFLD010000039        28751 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.039, whole genome shotgun sequence.
ACCESSION   DAZFLD010000039 DAZFLD010000000
VERSION     DAZFLD010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28751
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.039"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            63..1016
                     /gene="ybhD"
                     /locus_tag="ACHKBL_001431"
     CDS             63..1016
                     /gene="ybhD"
                     /locus_tag="ACHKBL_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308823.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO5818321.1"
                     /translation="MKHELSSMKAFVILAESSSFNNAAKLLNITQPALTRRIKKMEED
                     LHIQLFERTTRKVTLTKAGKRLLPEARELIKKFDETLFNIRDMNAYHRGMVTLACIPT
                     AVFYFLPLAIGKFNELYPNIKVRILEQGTNNCMESVLCNESDFGINMNNVTNSSIDFT
                     PLVNEPFVLACRRDHPLAKKQLVEWQELVGYKMIGVRSSSGNRLLIEQQLADKPWKLD
                     WFYEVRHLSTSLGLVEAGLGISALPGLAMPHAPYSSIIGIPLVEPVIRRTLGIIRRKD
                     AVLSPAAERFFALLINLWTDDKDNLWTNIVERQRHALQEIG"
     gene            complement(1057..2052)
                     /gene="pgl"
                     /locus_tag="ACHKBL_001432"
     CDS             complement(1057..2052)
                     /gene="pgl"
                     /locus_tag="ACHKBL_001432"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HFO5818322.1"
                     /translation="MKQTVYIASPESQQIHVWNLNHEGALTLTQVVDVPGQVQPMVVS
                     PDKRYLYVGVRPEFRVLAYRIAPDDGALTFAAESALPGSPTHISTDHQGQFVFVGSYN
                     AGNVSVTRLEDGLPVGVVDVVEGLDGCHSANISPDNRTLWVPALKQDRICLFTVSEDG
                     HLVAQDPAEVTTVEGAGPRHMVFHPNEQYAYCVNELNSSVDVWELKDPHGNIECVQTL
                     DMMPENFSDTRWAADIHITPDGRHLYACDRTASLITVFSVSEDGSVLSKEGFQPTETQ
                     PRGFNVDHSGKYLIAAGQKSHHISVYEIVGEQGLLHEKGRYAVGQGPMWVVVNAH"
     gene            2207..3025
                     /gene="ybhA"
                     /locus_tag="ACHKBL_001433"
     CDS             2207..3025
                     /gene="ybhA"
                     /locus_tag="ACHKBL_001433"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyridoxal
                     phosphate/fructose-1,6-bisphosphate phosphatase"
                     /protein_id="HFO5818323.1"
                     /translation="MTTRVIALDLDGTLLTPKKTLLPSSIEALARAREAGYQLIIVTG
                     RHHVAIHPFYQALALDTPAICCNGTYLYDYHAKTVLEADPMPVNKALQLIEMLNEHHI
                     HGLMYVDDAMVYEHPTGHVIRTSNWAQTLPPEQRPTFTQVASLAETAQQVNAVWKFAL
                     THDDLPQLQHFGKHVEHELGLECEWSWHDQVDIARGGNSKGKRLTKWVEAQGWSMENV
                     VAFGDNFNDISMLEAAGTGVAMGNADDAVKARANIVIGDNTTDSIAQFIYSHLI"
     gene            complement(3026..4084)
                     /gene="modC"
                     /locus_tag="ACHKBL_001434"
     CDS             complement(3026..4084)
                     /gene="modC"
                     /locus_tag="ACHKBL_001434"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HFO5818324.1"
                     /translation="MLELNFSQTLGNHCLTINETLPANGITAIFGVSGAGKTSLINAI
                     SGLTRPQKGRIVLNGRVLNDAEKGICLTPEKRRVGYVFQDARLFPHYKVRGNLRYGMA
                     KSMVDQFDKLVALLGIEPLLDRLPGSLSGGEKQRVAIGRALLTAPELLLLDEPLASLD
                     IPRKRELLPYLQRLTREINIPMLYVSHSLDEILHLADRVMVLENGQVKAFGALEEVWG
                     SSVMNPWLPKEQQSSILKVTVLEHHPHYAMTALALGDQHLWVNKLDEPLQAALRIRIQ
                     ASDVSLVLQPPQQTSIRNVLRAKVVNSYDDNGQVEVELEVGGKTLWARISPWARDELA
                     IKPGLWLYAQIKSVSITA"
     gene            complement(4087..4776)
                     /gene="modB"
                     /locus_tag="ACHKBL_001435"
     CDS             complement(4087..4776)
                     /gene="modB"
                     /locus_tag="ACHKBL_001435"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HFO5818325.1"
                     /translation="MILTDPEWQAVLLSLKVSSLAVLFSLPFGIFFAWLLVRCTFPGK
                     ALLDSVLHLPLVLPPVVVGYLLLVSMGRRGFIGERLYDWFGITFAFSWRGAVLAAAVM
                     SFPLMVRAIRLALEGVDVKLEQAARTLGAGRWRVFFTITLPLTLPGIIVGTVLAFARS
                     LGEFGATITFVSNIPGETRTIPSAMYTLIQTPGGESGAARLCIISIALAMISLLISEW
                     LARISRERAGR"
     gene            complement(4776..5549)
                     /gene="modA"
                     /locus_tag="ACHKBL_001436"
     CDS             complement(4776..5549)
                     /gene="modA"
                     /locus_tag="ACHKBL_001436"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO5818326.1"
                     /translation="MARKWLNLFAGAALSFAVAGNALADEGKITVFAAASLTNAMQDI
                     ATQYKKEKGVDVVSSFASSSTLARQIEAGAPADLFISADQKWMDYAVDKKAIDTASRQ
                     TLLGNSLVVVAPKASEQKDFTIDSKTNWTSLLNGGRLAVGDPEHVPAGIYAKEALQKL
                     GAWDTLSPKLAPAEDVRGALALVERNEAPLGIVYGSDAVASKGVKVVATFPEDSHKKV
                     EYPVAVVEGHNNATVKAFYDYLKGPQAAEIFKRYGFTTK"
     gene            complement(5715..5864)
                     /gene="acrZ"
                     /locus_tag="ACHKBL_001437"
     CDS             complement(5715..5864)
                     /gene="acrZ"
                     /locus_tag="ACHKBL_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump accessory protein AcrZ"
                     /protein_id="HFO5818327.1"
                     /translation="MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQ
                     PGQNH"
     gene            5993..6781
                     /gene="modE"
                     /locus_tag="ACHKBL_001438"
     CDS             5993..6781
                     /gene="modE"
                     /locus_tag="ACHKBL_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum-dependent transcriptional regulator"
                     /protein_id="HFO5818328.1"
                     /translation="MQAEILLTLKLQQKLFADPRRISLLKHIALSGSISQGAKDAGIS
                     YKSAWDAINEMNQLSEHILVERATGGKGGGGAVLTRYGQRLIQLYDLLAQIQQKAFDV
                     LSDDDALPLNSLLAAISRFSLQTSARNQWFGTITARNHDDVQQHVDVLLADGKTRLKV
                     AITAQSGARLGLDEGKEVLILLKAPWVGITQDEAVAQNADNQLPGIISHIERGAEQCE
                     VLMALPDGQTLCATVPVNEATSLQQGQNVTAYFNADSVIIATLC"
     gene            6849..8321
                     /gene="modF"
                     /locus_tag="ACHKBL_001439"
     CDS             6849..8321
                     /gene="modF"
                     /locus_tag="ACHKBL_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HFO5818329.1"
                     /translation="MSSLQILQGTFRLSDTKTLQLPQLTLNAGDSWAFVGSNGSGKSA
                     LARAQAGELPLLKGERQSQFSHITRLSFEQLQKLVSDEWQRNNTDMLGPGEDDTGRTT
                     AEIIQDEVKDAPRCMQLAQQFGITALLDRRFKYLSTGETRKTLLCQALMSEPDLLILD
                     EPFDGLDVASRQQLAELLASLHQSGITLVLVLNRFDEIPEFVQFAGVLADCTLAETGA
                     KEELLQQALVAQLAHSEQLEGVQLPEPDEPSARHALPANEPRIVLNNGVVSYNDRPIL
                     NNLSWQVNPGEHWQIVGPNGAGKSTLLSLITGDHPQGYSNDLTLFGRRRGSGETIWDI
                     KKHIGYVSSSLHLDYRVSTTVRNVILSGYFDSIGIYQAVSDRQQKLVQQWLDILGIDK
                     RTADAPFHSLSWGQQRLALIVRALVKHPTLLILDEPLQGLDPLNRQLIRRFVDVLISE
                     GETQLLFVSHHAEDAPACITHRLEFVPDGDFYRYALTKIN"
     gene            8582..9598
                     /gene="galE"
                     /locus_tag="ACHKBL_001440"
     CDS             8582..9598
                     /gene="galE"
                     /locus_tag="ACHKBL_001440"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308814.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HFO5818330.1"
                     /translation="MRVLVTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVI
                     ERLGGKHPTFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVGESVQKPLEYYDNNVN
                     GTLRLISAMRAANVKNFIFSSSATVYGDQPKIPYVESFPTGTPQSPYGKSKLMVEQIL
                     TDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIF
                     GNDYPTEDGTGVRDYIHVMDLADGHVVAMEKLANKPGVHIYNLGAGVGSSVLDVVNAF
                     SKACGKPVNYHFAPRREGDLPAYWADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQ
                     GYPD"
     gene            9608..10654
                     /gene="galT"
                     /locus_tag="ACHKBL_001441"
     CDS             9608..10654
                     /gene="galT"
                     /locus_tag="ACHKBL_001441"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HFO5818331.1"
                     /translation="MTQFNPVDHPHRRYNPLTGQWILVSPHRAKRPWQGAQETPAKQV
                     LPAHDPDCFLCAGNVRVTGDKNPDYTGTYVFTNDFAALMSDTPDAPESHDPLMRCQSA
                     RGTSRVICFSPDHSKTLPELSVAALTEIVKTWQEQTAELGKTYPWVQVFENKGAAMGC
                     SNPHPHGQIWANSFLPNEAEREDRLQKEYFAEQKSPMLVDYVQRELADGSRTVVETEH
                     WLAVVPYWAAWPFETLLLPKAHVLRITDLTDAQRSDLALALKKLTSRYDNLFQCSFPY
                     SMGWHGAPFNGEENQHWQLHAHFYPPLLRSATVRKFMVGYEMLAETQRDLTAEQAAER
                     LRAVSDIHFRESGV"
     gene            10658..11806
                     /gene="galK"
                     /locus_tag="ACHKBL_001442"
     CDS             10658..11806
                     /gene="galK"
                     /locus_tag="ACHKBL_001442"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HFO5818332.1"
                     /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL
                     PCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLDATIVAHENYQWANYVRGVVKH
                     LQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQE
                     AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL
                     VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV
                     EAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGG
                     GFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC"
     gene            11800..12840
                     /gene="galM"
                     /locus_tag="ACHKBL_001443"
     CDS             11800..12840
                     /gene="galM"
                     /locus_tag="ACHKBL_001443"
                     /EC_number="5.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004034 - aldose 1-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HFO5818333.1"
                     /translation="MLNETPALAPDGQPYRLLTLRNNAGMVVTLMDWGATLLSARIPF
                     SDGSVREALLGCASPECYQDQAAFLGASIGRYANRIANSRYTFDGETVTLSPSQGVNQ
                     LHGGPEGFDKRRWQIVNQNDRQVLFALSSDDGDQGFPGNLGATVQYRLTDDNRISITY
                     RATVDKPCPVNMTNHVYFNLDGEQSDVRNHKLQILADEYLPVDEGGIPHDGLKSVAGT
                     SFDFRSAKIIASEFLADDDQRKVKGYDHAFLLQAKGDGKKVAAHVWSADEKLQLKVYT
                     TAPALQFYSGNYLGGTPSRGTEPYTDWQGLALESEFLPDSPNHPEWPQPDCFLRPGEE
                     YSSLTEYQFIAQ"
     gene            13043..13795
                     /gene="gpmA"
                     /locus_tag="ACHKBL_001444"
     CDS             13043..13795
                     /gene="gpmA"
                     /locus_tag="ACHKBL_001444"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFO5818334.1"
                     /translation="MAVTKLVLVRHGESQWNKENRFTGWYDVDLSEKGVSEAKAAGKL
                     LKEEGYSFDFAYTSVLKRAIHTLWNVLDELDQAWLPVEKSWKLNERHYGALQGLNKAE
                     TAEKYGDEQVKQWRRGFAVTPPELTKDDERYPGHDPRYAKLSEKELPLTESLALTIDR
                     VIPYWNETILPRMKSGERVIIAAHGNSLRALVKYLDNMSEEEILELNIPTGVPLVYEF
                     DENFKPLKRYYLGNADEIAAKAAAVANQGKAK"
     gene            complement(13943..14995)
                     /gene="aroG"
                     /locus_tag="ACHKBL_001445"
     CDS             complement(13943..14995)
                     /gene="aroG"
                     /locus_tag="ACHKBL_001445"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HFO5818335.1"
                     /translation="MNYQNDDLRIKEIKELLPPVALLEKFPATENAANTVAHARKAIH
                     KILKGNDDRLLVVIGPCSIHDPVAAKEYATRLLTLREELQDELEIVMRVYFEKPRTTV
                     GWKGLINDPHMDNSFQINDGLRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAINAAGAPHCFLSVTKWGHS
                     AIVNTSGNGDCHIILRGGKEPNYSAKHVAEVKEGLNKAGLPAQVMIDFSHANSSKQFK
                     KQMDVCADVCQQIAGGEKAIIGVMVESHLVEGNQSLESGEPLAYGKSITDACIGWDDT
                     DALLRQLANAVKARRG"
     gene            15311..15691
                     /gene="ybgS"
                     /locus_tag="ACHKBL_001446"
     CDS             15311..15691
                     /gene="ybgS"
                     /locus_tag="ACHKBL_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgS-like family protein"
                     /protein_id="HFO5818336.1"
                     /translation="MKMTKLATLFLTATLSLASGAALAADSGAQSNNGQANAAADAGQ
                     VAPDARENVAPNNVDNNGVNTGSGGTMLHPDGSSMNNDGMTKDEEHKNTMCKDGRCPD
                     INKKVQTGDGINNDVDTKTDGTTQ"
     gene            15805..16746
                     /gene="zitB"
                     /locus_tag="ACHKBL_001447"
     CDS             15805..16746
                     /gene="zitB"
                     /locus_tag="ACHKBL_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005385 - zinc ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc transporter ZitB"
                     /protein_id="HFO5818337.1"
                     /translation="MAHSHSHTSSHLPEDNNARRLLYAFGVTAGFMLVEVIGGFLSGS
                     LALLADAGHMLTDTAALLFALLAVQFSRRPPTIRHTFGWLRLTTLAAFVNAIALVVIT
                     ILIVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHV
                     LGDLLGSVGAIIAALIIIWTGWTPADPILSILVSLLVLRSAWRLLKDSVNELLEGAPV
                     SLDIAELKRRMCREIPEVRNVHHVHVWMVGEKPVMTLHVQVIPPHDHDALLDQIQHYL
                     MDHYQIEHATIQMEYQPCHGPDCHLNEGVSGHSHHHH"
     gene            complement(16743..17462)
                     /gene="pnuC"
                     /locus_tag="ACHKBL_001448"
     CDS             complement(16743..17462)
                     /gene="pnuC"
                     /locus_tag="ACHKBL_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HFO5818338.1"
                     /translation="MDFFSVQNILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASLEK
                     ISNYFFGLINVTLFGIIFFQIQLYASLLLQMFFFAANIYGWYAWSRQTSQNEAELKIR
                     WLPLPKALSWLAVCVVSIGLMTVFINPVFAFLTRVAVMIMQALGLQVAMPELQPDAFP
                     FWDSCMMVLSIVAMILMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYIILTFIA
                     LNGSRMWINSARERGSRALSH"
     gene            complement(17500..18543)
                     /gene="nadA"
                     /locus_tag="ACHKBL_001449"
     CDS             complement(17500..18543)
                     /gene="nadA"
                     /locus_tag="ACHKBL_001449"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HFO5818339.1"
                     /translation="MSVMFDPDTAIYPFPPKPTPLSIDEKAYYREKIKRLLKERNAVM
                     VAHYYTDPEIQQLAEETGGCISDSLEMARFGAKHPASTLLVAGVRFMGETAKILSPEK
                     TILMPTLQAECSLDLGCSVEEFNAFCDAHPDRTVVVYANTSAAVKARADWVVTSSIAV
                     ELIDHLDSLGEKIIWAPDKHLGRYVQKQTGADILCWQGACIVHDEFKTQALTRLQEEY
                     PDAAILVHPESPQAIVEMADAVGSTSQLIAAAKTLPHQRLIVATDRGIFYKMQQAVPV
                     KELLEAPTAGEGATCRSCAHCPWMAMNGLQAIAEALEQEGSNHEVHVDERLRERALVP
                     LNRMLDFAATLRG"
     gene            complement(18791..18866)
                     /locus_tag="ACHKBL_001450"
     tRNA            complement(18791..18866)
                     /locus_tag="ACHKBL_001450"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18831..18833),aa:Lys,seq:ttt)
     gene            complement(18870..18945)
                     /locus_tag="ACHKBL_001451"
     tRNA            complement(18870..18945)
                     /locus_tag="ACHKBL_001451"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18910..18912),aa:Val,seq:tac)
     gene            complement(18997..19072)
                     /locus_tag="ACHKBL_001452"
     tRNA            complement(18997..19072)
                     /locus_tag="ACHKBL_001452"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19037..19039),aa:Lys,seq:ttt)
     gene            complement(19075..19150)
                     /locus_tag="ACHKBL_001453"
     tRNA            complement(19075..19150)
                     /locus_tag="ACHKBL_001453"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19115..19117),aa:Val,seq:tac)
     gene            complement(19286..19361)
                     /locus_tag="ACHKBL_001454"
     tRNA            complement(19286..19361)
                     /locus_tag="ACHKBL_001454"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19326..19328),aa:Lys,seq:ttt)
     gene            complement(19526..20317)
                     /gene="cpoB"
                     /locus_tag="ACHKBL_001455"
     CDS             complement(19526..20317)
                     /gene="cpoB"
                     /locus_tag="ACHKBL_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0070206 - protein trimerization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CpoB"
                     /protein_id="HFO5818340.1"
                     /translation="MSSNFRHQLLSLSLLVGIAAPWAAFAQAPISSVGSGSVEDRVTQ
                     LERISNAHSQLLTQLQQQLSDNQSDIDSLRGQIQENQYQLNQVVERQKQILLQIDSLS
                     SGGAAAQSTSGDQSGAAASTTPTADAGTANAGAPVKSGDANTDYNAAIALVQDKSRQD
                     EAIVAFQNFIKNYPDSTYLPNANYWLGQLNYNKGKKDDAAYYFASVVKNYPKSPKAAD
                     AMFKVGVIMQDKGDTAKAKAVYQQVISKYPGTDGAKQAQKRLNAM"
     gene            complement(20327..20848)
                     /gene="pal"
                     /locus_tag="ACHKBL_001456"
     CDS             complement(20327..20848)
                     /gene="pal"
                     /locus_tag="ACHKBL_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HFO5818341.1"
                     /translation="MQLNKVLKGLMIALPVMAIAACSSNKNASNDGSEGMLGAGTGMD
                     ANGGNGNMSSEEQARLQMQQLQQNNIVYFDLDKYDIRSDFAQMLDAHANFLRSNPSYK
                     VTVEGHADERGTPEYNISLGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDE
                     AAYSKNRRAVLVY"
     gene            complement(20883..22175)
                     /gene="tolB"
                     /locus_tag="ACHKBL_001457"
     CDS             complement(20883..22175)
                     /gene="tolB"
                     /locus_tag="ACHKBL_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HFO5818342.1"
                     /translation="MKQALRVAFGFLILWASVLHAEVRIVIDSGVDSGRPIGVVPFQW
                     AGPGAAPEDIGGIVAADLRNSGKFNPLDRARLPQQPGSAQEVQPAAWSALGIDAVVVG
                     QVTPNPDGSYNVAYQLVDTGGAPGTVLAQNSYKVNKQWLRYAGHTASDEVFEKLTGIK
                     GAFRTRIAYVVQTNGGQFPYELCVSDYDGYNQFVVHRSPQPLMSPAWSPDGSKLAYVT
                     FESGRSALVIQTLANGAVRQVASFPRHNGAPAFSPDGSKLAFALSKTGSLNLYVMDLA
                     SGQIRQVTDGRSNNTEPTWFPDSQNLAFTSDQAGRPQVYKVNINGGAPQRITWEGSQN
                     QDADVSSDGKFMVMVSSNGGQQHIAKQDLATGGVQVLSSTFLDETPSLAPNGTMVIYS
                     SSQGMGSVLNLVSTDGRFKARLPATDGQVKFPAWSPYL"
     gene            complement(22308..23573)
                     /gene="tolA"
                     /locus_tag="ACHKBL_001458"
     CDS             complement(22308..23573)
                     /gene="tolA"
                     /locus_tag="ACHKBL_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0019534 - toxin transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HFO5818343.1"
                     /translation="MSKATEQNDKLKRAIIISAVLHVILFAALIWSSFDENIEASAGG
                     GGGSSIDAVMVDSGAVVEQYKRMQSQESSAKRSDEQRKMKEQQAAEELREKQAAEQER
                     LKQLEKERLAAQEQKKQAEEAAKQAELKQKQAEEAAAKAAADAKAKAEADAKAAEEAA
                     KKAAADAKKKAEAEAAKAAAEAQKKAEAAAAALKKKAEAAEAAAAEARKKAATEAAEK
                     AKAEAEKKAAAEKAAADKKAAAEKAAADKKAAEKAAAEKAAADKKAAAEKAAADKKAA
                     AAKAAAEKAAAAKAAAEADDIFGELSSGKNAPKTGGGAKGNNASPAGSGNTKNNGASG
                     ADINNYAGQIKSAIESKFYDASSYAGKTCTLRIKLAPDGMLLDIKPEGGDPALCQAAL
                     AAAKLAKIPKPPSQAVYEVFKNAPLDFKP"
     gene            complement(23638..24066)
                     /gene="tolR"
                     /locus_tag="ACHKBL_001459"
     CDS             complement(23638..24066)
                     /gene="tolR"
                     /locus_tag="ACHKBL_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HFO5818344.1"
                     /translation="MARARGRGRRDLKSEINIVPLLDVLLVLLLIFMATAPIITQSVE
                     VDLPDATESQAVSSNDNPPVIVEVSGIGQYTVVVEKDRLERLPPEQVVAEVSSRFKAN
                     PKTVFLIGGAKDVPYDEIIKALNLLHSAGVKSVGLMTQPI"
     gene            complement(24070..24762)
                     /gene="tolQ"
                     /locus_tag="ACHKBL_001460"
     CDS             complement(24070..24762)
                     /gene="tolQ"
                     /locus_tag="ACHKBL_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolQ"
                     /protein_id="HFO5818345.1"
                     /translation="MTDMNILDLFLKASLLVKLIMLILIGFSIASWAIIIQRTRILNA
                     AAREAEAFEDKFWSGIELSRLYQESQGKRDNLTGSEQIFYSGFKEFVRLHRANSHAPE
                     AVVEGASRAMRISMNRELENLETHIPFLGTVGSISPYIGLFGTVWGIMHAFIALGAVK
                     QATLQMVAPGIAEALIATAIGLFAAIPAVMAYNRLNQRVNKLELNYDNFMEEFTAILH
                     RQAFTVSESNKG"
     gene            complement(24759..25163)
                     /gene="ybgC"
                     /locus_tag="ACHKBL_001461"
     CDS             complement(24759..25163)
                     /gene="ybgC"
                     /locus_tag="ACHKBL_001461"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HFO5818346.1"
                     /translation="MNTTLFRWPVRVYYEDTDAGGVVYHASYVAFYERARTEMLRHHH
                     FSQQALMAERVAFVVRKMTVEYYAPARLDDMLEIQTEITSMRGTSLVFTQRIVNAENT
                     LLNEAEVLVVCVDPLKMKPRALPKSIVAEFKQ"
     gene            complement(25313..25606)
                     /gene="ybgE"
                     /locus_tag="ACHKBL_001462"
     CDS             complement(25313..25606)
                     /gene="ybgE"
                     /locus_tag="ACHKBL_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HFO5818347.1"
                     /translation="MSKIIATLYAVMDKRPLRALSFVMALLLAGCMFWDPSRFAAKTS
                     ELEIWHGLLLMWAVCAGVIHGVGFRPQKVLWQGIFCPLLADIVLIVGLIFFFF"
     gene            complement(25606..25719)
                     /gene="cydX"
                     /locus_tag="ACHKBL_001463"
     CDS             complement(25606..25719)
                     /gene="cydX"
                     /locus_tag="ACHKBL_001463"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HFO5818348.1"
                     /translation="MWYFAWILGTLLACSFGVITALALEHVESGKAGQEDI"
     gene            complement(25734..26873)
                     /gene="cydB"
                     /locus_tag="ACHKBL_001464"
     CDS             complement(25734..26873)
                     /gene="cydB"
                     /locus_tag="ACHKBL_001464"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HFO5818349.1"
                     /translation="MIDYEVLRFIWWLLVGVLLIGFAVTDGFDMGVGMLTRFLGRNDT
                     ERRIMINSIAPHWDGNQVWLITAGGALFAAWPMVYAAAFSGFYVAMILVLASLFFRPV
                     GFDYRSKIEETRWRNMWDWGIFIGSFVPPLVIGVAFGNLLQGVPFNVDEYLRLYYTGN
                     FFHLLNPFGLLAGVVSVGMIITQGATYLQMRTVGELHLRTRATAQVAALVTLVCFALA
                     GVWVMYGIDGYVVKSTMDHYAASNPLNKEVVREAGAWLVNFNNTPILWAIPALGVVLP
                     LLTILTARMDKAAWAFVFSSLTLACIILTAGIAMFPFVMPSSTMMNASLTMWDATSSQ
                     LTLNVMTWVAVVLVPIILLYTAWCYWKMFGRITKEDIERNTHSLY"
     gene            complement(26889..28457)
                     /gene="cydA"
                     /locus_tag="ACHKBL_001465"
     CDS             complement(26889..28457)
                     /gene="cydA"
                     /locus_tag="ACHKBL_001465"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415261.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HFO5818350.1"
                     /translation="MLDIVELSRLQFALTAMYHFLFVPLTLGMAFLLAIMETVYVLSG
                     KQIYKDMTKFWGKLFGINFALGVATGLTMEFQFGTNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWDRLGKVQHMCVTWLVALGSNLSALWILVANGWMQNPIASDFN
                     FETMRMEMVSFSELVLNPVAQVKFVHTVASGYVTGAMFILGISAWYMLKGRDFAFAKR
                     SFAIAASFGMAAVLSVIVLGDESGYEMGDVQKTKLAAIEAEWETQPAPAAFTLFGIPD
                     QEEETNKFAIQIPYALGIIATRSVDTPVIGLKELMVQHEERIRNGMKAYSLLEQLRSG
                     STDQAVRDQFNSMKKDLGYGLLLKRYTPNVADATEAQIQQATKDSIPRVAPLYFAFRI
                     MVACGFLLLAIIALSFWSVIRNRIGEKKWLLRAALYGIPLPWIAVEAGWFVAEYGRQP
                     WAIGEVLPTAVANSSLTAGDLIFSMVLICGLYTLFLVAELFLMFKFARLGPSSLKTGR
                     YHFEQSSTTTQPAR"
BASE COUNT         7167 a         7648 c         7065 g         6871 t
ORIGIN      
        1 tgcagttaaa atttttaact gccagtcacc caaaatcgtc gcttccattt tccggagtaa
       61 taatgaagca tgaattatca agtatgaagg catttgtcat actggcagag tccagttcat
      121 ttaacaatgc tgctaaatta ctcaatatta cgcaacctgc attaacgcga agaataaaaa
      181 agatggagga agatttacac attcaactat ttgagcgtac aactcgcaag gttacgttaa
      241 caaaagcagg aaaaaggttg ctcccggagg cgcgggaatt aataaaaaaa ttcgatgaaa
      301 cgctttttaa tattcgtgat atgaatgctt atcatcgtgg tatggtgacg ttagcgtgta
      361 ttccgaccgc agtattttat tttttaccgc tggcaatcgg taaatttaat gagctgtatc
      421 ccaatattaa agtgcggatt ctggaacaag gcacgaataa ttgcatggaa tcggtgctgt
      481 gtaacgaatc agactttggc ataaacatga acaacgtcac aaattcatcc attgatttta
      541 ccccactggt caacgagccg ttcgtgctgg cctgtcggcg tgaccatcca ttggccaaaa
      601 agcagcttgt agaatggcag gagttagtgg gttacaaaat gattggcgta cgctcctcca
      661 gcggcaaccg actattgata gaacaacagc tagccgacaa gccctggaag ctggactggt
      721 tttacgaagt gcgtcatctt tcgacgtcat tagggctggt ggaggcggga ctggggattt
      781 cggcgcttcc tggtctggca atgccccatg cgccctattc ttcgattatt ggtatcccgc
      841 tggtggaacc ggttatacgt cggacattgg ggattattcg ccgcaaagat gccgtgcttt
      901 ctccggcagc tgaacgcttt tttgccttgc tgattaattt gtggactgac gataaagaca
      961 atttgtggac caatatcgtc gaacgccaga gacatgcgtt acaggagata ggttaataca
     1021 ccggcgcaga agagactgcg ccgggtaaat cagcggttag tgtgcgttaa ccaccaccca
     1081 cattggtccc tgcccgaccg catagcggcc tttttcatgc agtagcccct gctcgccaac
     1141 aatttcgtat accgagatgt ggtgagattt ttgcccggcg gcaatcagat acttgccgct
     1201 gtgatcaaca ttgaagccgc gcggctgggt ttcagttggc tggaagcctt ctttactcaa
     1261 cacgctgcca tcttccgaaa cgctgaaaac agtaatcaga ctggcggtac ggtcgcaggc
     1321 gtataaatga cgaccatccg gggtgatatg aatatcagcc gcccaacggg tgtcggagaa
     1381 gttttccggc atcatatcca gcgtctggac acattcgata ttgccatgcg gatctttcag
     1441 ttcccagaca tccactgagc tgtttaactc attgacgcaa tacgcatatt gttcgtttgg
     1501 atggaatacc atatgacgcg ggccggcccc ttcaacggta gtcacttccg cagggtcctg
     1561 cgccaccaga tgaccatcct cgctgacagt aaacaggcaa atgcgatcct gctttaatgc
     1621 cggaacccac agcgtacggt tgtccggtga gatattggcg gaatggcaac cgtccagccc
     1681 ctcgaccaca tcgacgacgc ccactggcag gccatcttcc agacgcgtta cgctcacgtt
     1741 acccgcattg taagaaccga caaagacaaa ctgcccctgg tgatcggtgg aaatatgcgt
     1801 cggactaccc ggcagcgcag actctgcggc aaaggtcagc gcgccatcgt ccggggcgat
     1861 acgatacgcc aggacgcgaa actcagggcg aacaccaaca tagagataac gtttgtccgg
     1921 gctgaccacc atcggctgca cctgccccgg cacgtcgaca acctgtgtca gcgtcagtgc
     1981 gccttcatga ttcaggttcc agacgtgaat ttgctggctc tcagggctgg cgatataaac
     2041 tgtttgcttc atgaatgctc ctttgcattt agcaactgac tgtaacagct aaaattagtc
     2101 gcttttggcg gtgaatgctg aattaaaacg cagaattttg tccgatagtg gtatcggtgt
     2161 accattcgcg cagacgaaaa ttcttaacct taatctggaa tacgccatga ccacacgcgt
     2221 gattgctctc gacttagacg gcaccttatt gaccccgaaa aagaccttgc ttccatcatc
     2281 gatagaagcc ctggcccgcg ctcgcgaagc aggctatcaa ttaatcatcg tcacaggtcg
     2341 ccatcacgtc gctattcatc ctttttatca ggcgctggcg ctggatacac ctgctatttg
     2401 ctgtaatggc acctatttgt atgattatca tgcaaaaaca gtgctggaag cggacccaat
     2461 gcccgttaat aaagccctgc aactcattga gatgctgaat gaacaccaca ttcacggtct
     2521 gatgtatgtc gatgatgcaa tggtctatga gcacccgacc ggacatgtca ttcgcacgtc
     2581 taactgggcg caaaccctgc cgccggaaca gcgtccgact ttcacacaag tcgcttctct
     2641 ggctgaaacg gcgcaacaag ttaacgccgt atggaagttc gccctgacgc acgatgacct
     2701 gccgcaattg cagcattttg gtaagcatgt cgaacatgaa ctgggactgg agtgtgaatg
     2761 gtcctggcac gatcaggttg atattgcacg cggcggcaac agcaaaggta aacgtttgac
     2821 gaaatgggtt gaggcgcaag gctggtcgat ggaaaacgtc gtggccttcg gcgataactt
     2881 caatgatatt agtatgctgg aagccgctgg tacaggcgtg gcgatgggca acgccgatga
     2941 cgcagtaaaa gcgcgcgcca acattgtgat tggtgataac accaccgaca gcattgccca
     3001 gttcatttat agccacctga tttaatcagg cggttatcga cacactttta atttgcgcat
     3061 acagccacag gccaggtttg atcgccagtt catccctggc ccacgggctg atacgcgccc
     3121 acagcgtttt accgccgact tccagctcca cttccacctg gccgttgtcg tcataactat
     3181 taaccacttt tgcccgcaat acgttacgaa tgctggtttg ctgcggcggt tgtaaaacca
     3241 gcgagacatc ggaagcctga atgcggatgc gtagcgcagc ttgcagcggt tcgtccagct
     3301 tattgaccca caaatgctga tcgcccagcg ccagcgcggt catcgcgtaa tgcggatgat
     3361 gctcaagcac cgtcactttc agaatgctac tttgttgctc tttcggcagc cacggattca
     3421 tcacgctact gccccacact tcctccagcg cgccaaaggc tttcacctga ccgttttcca
     3481 gtaccatcac tctgtctgcc agatggagga tctcatccag cgaatggctg acatacaaca
     3541 tcggaatgtt gatttcccgt gtcagccgtt gcagataagg caacagttcg cgtttacgcg
     3601 gaatatccag tgacgccagc ggttcatcca gcaacagcaa ttccggtgct gtcagcaaag
     3661 cccgaccaat cgccacgcgc tgtttttcac ctccggacag gctgcctggt aaacggtcaa
     3721 gcaacggttc aatgcctaaa agcgccacca gcttatcgaa ctgatcgacc atacttttcg
     3781 ccatgccgta gcgcagattg ccacgcactt tgtaatgcgg gaacagccgc gcatcctgaa
     3841 aaacatagcc aacgcgacgc ttttccggcg tcaggcagat acctttttcg gcatcattta
     3901 gtacccgccc attgaggaca atccgccctt tttgcgggcg cgtcagtcca ctgatggcgt
     3961 taatcagcga agtttttccg gcaccggaga cgccaaagat agcagtgatg ccattggcgg
     4021 gcagcgtttc attaatggtc aggcaatggt tgcccaacgt ctgggaaaaa ttcagttcca
     4081 gcatgattag cgccccgccc gttcacggct gattctggcc agccattctg aaatcaacag
     4141 ggagatcatc gccagtgcaa tagaaataat gcacagtctc gccgctccac tttccccgcc
     4201 gggggtctgg atcagggtat acatggcaga aggaatggtt cgcgtttcac caggaatgtt
     4261 cgacacaaag gtgatggttg caccaaactc accgagagaa cgagcaaaag ccagtaccgt
     4321 accaacaata atgcccggta aggtcagcgg taacgtgata gtaaagaaaa cgcgccagcg
     4381 cccggcaccc agtgttcttg cggcctgttc cagtttgaca tcaacccctt ccagcgccag
     4441 acgaattgcc cgcaccatca gcggaaacga catgacggcg gcagcgagaa ccgcgccgcg
     4501 ccagctaaag gcgaaggtaa tgccaaacca gtcatacaga cgttcaccga taaatccgcg
     4561 ccgtcccatc gaaactaata ataagtaacc gacgaccacg ggcggtaaca ccagcggtag
     4621 atgcagtacg ctgtcgagca gagctttgcc cggaaacgtg caacgcacca gtaaccaggc
     4681 aaaaaagatc ccaaacggca ggctaaacag cacagccagg gaagaaactt tcaggcttag
     4741 taaaaccgcc tgccattctg gatcggtcag tatcattact tggttgtaaa tccgtaacgt
     4801 ttaaagattt ccgcagcctg cggtcccttc agataatcat aaaatgcttt caccgtcgca
     4861 ttgttatgcc cttccacaac agcaaccgga tattccactt ttttatgcga atcttccggg
     4921 aaggtggcaa ccacttttac ccctttgctg gcaactgcgt cagaaccgta aacaataccc
     4981 agcggcgctt cattgcgttc gaccaacgcc agcgcgccac gaacatcttc cgccggagcc
     5041 agtttcgggg agagcgtatc ccatgcgccc agtttttgca gtgcttcttt tgcataaatg
     5101 ccagcgggaa catgttccgg atcgccaacc gccagacgac cgccattcag cagcgaagtc
     5161 cagttggttt tgctgtcgat ggtgaaatct ttctgctcgc tggctttcgg tgctacaacg
     5221 accaggctat tgccgagcag tgtctggcgc gaagccgtat cgatcgcttt tttatcaacc
     5281 gcataatcca tccatttctg atcggcagaa ataaacagat ccgcaggcgc acccgcttca
     5341 atctgacggg ccaaagtaga tgacgaagcg aaagaagaaa ccacatccac gcctttctct
     5401 tttttatact gcgtagcaat gtcctgcatt gcgttagtca gtgatgcggc ggcgaacacc
     5461 gtgattttcc cttcatctgc cagtgcattg ccagcaacag cgaaagagag tgccgccccg
     5521 gcaaacaagt tcaaccattt acgagccatt ggtaacccct taatctaacg ttatgtaggc
     5581 gacaatataa cggctaactc agaattttcc cagcagttat tgctaacctt tgtgaggtag
     5641 atgagaaaaa atatcggcag gaaaagcagg aggttgagaa aaagaaatgc ccgactaagc
     5701 gggcattcag ggattcaatg attttgtccg ggctggtctt ttttaccaac accagaaaag
     5761 atgttgaata cttcaccaag accgtaaatc agacccagga tgatggccat cacgacaggt
     5821 accatgatta cggcgaatac cagacttttt aataactcta acatggtcaa ctccagatat
     5881 agtcatgaga ctattctaac cgctaagcaa agaaaagcac tccccttttg tgcggtcagc
     5941 tttgcgtggc gttcgttttc cgtcacaata agactttttc caggatattg ttatgcaggc
     6001 cgaaatcctt ctcaccctta agctccaaca aaaattattc gccgacccgc gccgcatttc
     6061 gctactaaaa cacattgcgc tttccggttc cattagccag ggagcgaaag atgccggtat
     6121 cagctataaa agcgcatggg atgccattaa cgagatgaat cagttaagtg agcatattct
     6181 ggttgagcgc gcaacaggcg gtaaaggtgg aggcggcgca gtactgaccc gctatggtca
     6241 gcgactgatt cagctctatg acttactggc gcaaatccag caaaaagcct tcgatgtgtt
     6301 aagtgacgat gacgccctgc cgctgaacag cctgctggcc gcgatctcac gtttttcact
     6361 gcaaaccagc gcccgtaacc aatggtttgg caccatcacc gcccgcaatc atgatgacgt
     6421 tcaacagcat gttgatgtct tactggctga cggaaaaaca cgcctgaaag tcgcaattac
     6481 cgcacaaagc ggcgcgcgtc tggggctgga tgaaggcaaa gaagtgttga tattactaaa
     6541 agcgccgtgg gtaggcatta ctcaggacga agcggtcgca caaaacgccg acaaccaatt
     6601 accgggtatt attagccata ttgagcgcgg cgcagagcag tgcgaagtat taatggcgct
     6661 acccgacggg caaacactgt gcgccacagt gccggtaaat gaagcgactt ctcttcagca
     6721 aggacagaat gtcacggcct actttaatgc cgacagcgtg attatcgcca cgctgtgcta
     6781 agcgtgttga caatttgtta tgaaacacgt atccctgtca gtaatcgctg cacaaaatgg
     6841 gatataaaat gtcatcgttg caaattttgc aaggcacgtt tcgtcttagc gacacaaaaa
     6901 cgctgcaatt gcctcagcta acgttaaacg cgggtgatag ttgggcgttt gtcggttcga
     6961 atggaagcgg gaaatcggcg ctggcccgcg cgcaggcggg ggaacttccg cttttgaaag
     7021 gtgaacggca aagccagttt tcccacatca ctcgtctctc cttcgagcaa ttgcaaaagc
     7081 tcgtcagcga cgaatggcag cggaataaca ccgatatgct cggccctggc gaagatgaca
     7141 ccgggcgtac cacggctgaa atcattcagg atgaagtaaa ggatgcaccg cgttgcatgc
     7201 aactggcgca gcagttcggt attaccgccc tcctcgaccg acgctttaaa tacctttcca
     7261 ctggcgagac gcgaaaaacc ctgctgtgtc aggcgctgat gtcggagcct gacttgttga
     7321 ttcttgatga gccgttcgat ggcctggatg ttgcctcacg tcagcagctg gctgagctac
     7381 tcgcctcgtt acatcagtcc ggtattactc tggtactggt gctcaatcgc ttcgatgaga
     7441 tcccggaatt tgtccagttt gctggcgtgc tggcggattg tacgttagcg gaaacgggcg
     7501 ctaaagagga actgctccag caagcactcg tcgcgcaact ggcacatagc gaacagcttg
     7561 aaggtgtgca actgccggag ccggatgaac cttcagcacg tcacgcctta cccgccaacg
     7621 aaccgcgcat tgtgctgaac aacggcgtgg tttcttataa cgatcgcccc attcttaata
     7681 accttagctg gcaggtgaat ccaggcgagc actggcaaat tgtcgggcca aatggcgcgg
     7741 gaaaatcgac gttattaagc ctgattactg gcgatcatcc gcaaggttac agcaacgatt
     7801 tgacgctttt cggacgacgt cgcggcagcg gcgaaaccat ctgggatatc aaaaagcata
     7861 tcggttacgt cagcagtagt ttgcatctgg attaccgggt cagcactacc gtgcgtaatg
     7921 tgatcctttc tggctatttt gattcgattg gcatttatca ggccgtttcg gatcgccagc
     7981 aaaaactggt gcagcagtgg ctggatattc tcggcattga taaacgcacg gctgacgctc
     8041 cgttccatag tctttcctgg ggacagcagc gtctggcgct gatcgtccgc gcgctggtga
     8101 aacatccgac gttgcttatt ctcgatgaac cactacaggg gcttgatccg ctcaatcgcc
     8161 agcttatccg ccggtttgtt gatgtgctga ttagcgaagg tgaaacgcaa ttgttgtttg
     8221 tttcgcacca tgctgaagat gcgcctgcct gtattaccca tcgtcttgag ttcgtgccgg
     8281 acggtgactt ttatcgctat gcgctgacaa aaataaactg agtcggtaat gctgactgta
     8341 ccggaggcgg cctaagcgcc ctctccggcc tacagttcga cgcatccagg catgaaaccg
     8401 cgactttttt cagataaaaa gctcaatcat tcataaaccc tctgttttat aatccattaa
     8461 tggcgcataa aaaacggcta aattcttgtg taaacgattc cactaattta ttccatgtca
     8521 cacttttcgc atctttgtta tgctatggtt atttcatacc ataagcctaa tggagcgaat
     8581 tatgagagtt ctggttaccg gtggtagcgg ttacattgga agtcatacct gtgtgcaatt
     8641 actgcaaaac ggtcatgatg tcatcattct tgataacctc tgtaacagta agcgcagcgt
     8701 actgcctgtt atcgagcgtt taggcggcaa acatccaacg tttgttgaag gcgatatccg
     8761 taacgaagcg ttgatgaccg agatcctgca cgatcacgct atcgacactg tgatccactt
     8821 cgccgggctg aaagccgttg gcgaatcggt acaaaaaccg cttgaatatt acgacaacaa
     8881 tgtcaacggt accctgcgcc tgattagcgc catgcgcgcc gcgaacgtca aaaactttat
     8941 ttttagctcc tccgccaccg tctatggcga tcagcccaaa attccatacg ttgaaagctt
     9001 cccgaccggc acaccgcaaa gcccttacgg caaaagcaaa ctgatggtgg aacagatcct
     9061 caccgatctg caaaaagccc aaccggactg gagcattgcc ctgctgcgct acttcaaccc
     9121 ggttggcgcg catccgtcgg gcgatatggg cgaagatccg caaggcattc cgaataacct
     9181 gatgccatac atcgcccagg ttgctgtagg ccgtcgcgac tcgctggcga tttttggtaa
     9241 cgattatccg accgaagacg gtactggcgt acgcgattac atccacgtaa tggatctggc
     9301 ggacggtcac gtcgtggcga tggaaaaact ggcgaacaag ccaggcgtac acatctataa
     9361 cctcggcgct ggcgtaggca gcagcgtgct ggacgtggtt aatgccttca gcaaagcctg
     9421 cggcaaaccg gttaattatc attttgcacc gcgtcgcgag ggcgaccttc cggcctactg
     9481 ggcggacgcc agcaaagccg accgtgaact gaactggcgc gtaacgcgca cactcgatga
     9541 aatggcgcag gacacctggc actggcagtc acgccatcca cagggatatc ccgattaagg
     9601 aacgaccatg acgcaattta atcccgttga tcatccacat cgccgctaca acccgctcac
     9661 cgggcaatgg attctggttt caccgcaccg cgctaagcgc ccctggcagg gggcgcagga
     9721 aacgccagcc aaacaggtgt tacctgctca tgatccagat tgcttcctct gcgcaggtaa
     9781 tgtgcgggtg acaggcgata aaaaccccga ttacaccggg acttacgttt tcactaatga
     9841 ctttgcggct ttgatgtctg acacaccaga tgcgccggaa agtcacgatc cgctgatgcg
     9901 ttgccagagc gcgcgtggca ccagtcgggt gatctgcttt tcaccggatc acagcaaaac
     9961 gttgccagaa ctcagcgttg cggcattgac ggaaatcgtc aaaacctggc aggagcaaac
    10021 cgcagaactg gggaaaacgt acccatgggt gcaggtcttt gaaaacaaag gcgcggcgat
    10081 gggctgctct aacccgcatc cgcacggaca gatttgggca aatagcttcc tgcctaacga
    10141 agctgagcgc gaagaccgtc tgcaaaaaga atatttcgcc gaacagaaat cgccaatgct
    10201 ggtggattat gttcagcgcg agctggcaga cggtagccgt accgttgtcg aaaccgaaca
    10261 ctggttagcc gttgtacctt actgggctgc ctggccgttc gaaacgctac tgctgcccaa
    10321 agcccacgtt ttacggatca ccgatttgac cgacgcccag cgcagcgatt tggcactggc
    10381 gttgaaaaag ctgaccagtc gttatgacaa cctcttccag tgctccttcc cctactctat
    10441 gggctggcac ggcgcgccat ttaatggcga agagaatcaa cactggcagc tgcacgcgca
    10501 cttttatccg cctctgttgc gctctgccac cgtacgtaaa tttatggttg gttatgaaat
    10561 gctggcagaa acccagcgag acctgaccgc agaacaggcc gcagagcgtt tgcgcgcagt
    10621 cagcgatatc cattttcgcg aatccggagt gtaagaaatg agtctgaaag aaaaaacaca
    10681 atctctgttt gccaacgcat ttggctaccc tgccactcac accattcagg cgcctggccg
    10741 cgtgaatttg attggtgaac acaccgacta caacgacggt ttcgttctgc cctgcgcgat
    10801 tgattatcaa accgtgatca gttgtgcacc acgcgatgac cgtaaagttc gcgtgatggc
    10861 agccgattat gaaaatcagc tcgacgagtt ttccctcgat gcgaccattg tcgcgcatga
    10921 aaactatcaa tgggctaact acgttcgtgg cgtggtgaaa catctgcaac tgcgtaacaa
    10981 cagcttcggc ggcgtggaca tggtgatcag cggcaatgtg ccgcagggtg ccgggttaag
    11041 ttcttccgct tcactggaag tcgcggtcgg aaccgtattg cagcagcttt atcatttgcc
    11101 gctggacggc gcacaaatcg cgcttaacgg tcaggaagca gaaaaccagt ttgttggctg
    11161 taactgcggg atcatggatc agctaatttc cgcactcggc aagaaagatc atgccttgct
    11221 gattgactgc cgctcactgg ggactaaagc agtttccatg ccgaaaggtg tggctgtcgt
    11281 catcattaac agtaacttca aacgtaccct ggttggcagc gaatacaaca cccgtcgtga
    11341 acagtgcgaa actggtgcgc gtttcttcca gcagccagcg ctgcgcgatg tcaccattga
    11401 agagttcaac gctgttgcgc atgaactgga cccaatcgtg gcgaaacgcg tgcgtcatat
    11461 cctgactgaa aacgcccgca ccgttgaagc tgccagcgca ctggagcaag gcgacctgaa
    11521 acgtatgggc gagttgatgg cggagtctca tgcctctatg cgcgatgatt tcgaaatcac
    11581 cgtgccgcaa attgacactc tggtagaaat cgtcaaagct gtgattggcg acaaaggtgg
    11641 cgtacgcatg accggcggcg gatttggcgg ctgtatcgtc gcgctgatcc cggaagagct
    11701 ggtgcctgcc gtacagcaag ctgtcgctga acaatatgaa gcaaaaacag gtattaaaga
    11761 gactttttac gtttgtaaac catcacaagg agcaggacag tgctgaacga aactcccgca
    11821 ctggcacccg atggtcagcc gtaccgactg ttaactttgc gtaacaacgc agggatggta
    11881 gtcacgctga tggactgggg tgcgacttta ctttccgccc gtattccgtt ttccgatggc
    11941 agcgtccgcg aggcgctgct cggctgtgcc agcccggaat gctatcagga tcaggccgca
    12001 tttctggggg cctctattgg tcgttatgcc aaccgtatcg ccaatagccg ttataccttt
    12061 gacggtgaaa ccgtgacgct ttcaccaagt cagggcgtta accagctgca cggcggcccg
    12121 gaagggttcg acaaacgtcg ctggcagatt gtgaaccaga acgatcgtca ggtgctgttt
    12181 gccctgagtt cagatgatgg cgatcagggc ttcccgggta atctcggagc gacggtacaa
    12241 tatcgtctga ccgacgataa ccgtatctct attacttatc gcgccacagt tgataaacct
    12301 tgcccggtga atatgactaa tcacgtctat ttcaatcttg acggcgagca gtctgacgtg
    12361 cgcaatcaca agttgcagat tctggcggac gaatatctgc cggttgatga aggcggcatt
    12421 ccccacgacg gcctgaaatc tgtcgccgga acgtcttttg atttccgcag cgccaaaatc
    12481 atcgccagtg agtttcttgc cgacgacgat cagcgcaaag tgaaaggtta cgatcacgcg
    12541 ttcttgttac aggccaaagg cgatggcaag aaagtggcgg cgcatgtctg gtcagcagat
    12601 gaaaaattgc agctgaaggt ctacaccacc gctccggctc tgcaattcta ctccggcaac
    12661 tacctcggcg gcacaccgtc gcggggaacc gaaccttaca ccgactggca agggctggcg
    12721 ttggaaagcg agtttctgcc ggacagcccg aaccaccctg aatggccgca accggactgt
    12781 ttcctgcgtc ctggcgaaga gtattccagc ctgacggaat atcagtttat tgctcagtaa
    12841 tgttaagccc tcgtcatgag ggctttatct tcatattgtt caaatcacca gcaaacaccg
    12901 acatatttgc aactcaatat tcacaacaac cttacactgc gacactattt tcgctatggt
    12961 tatgcgtaag cattgctgtt gcttcgtcac ggcaatataa tgagaattat tatcattaaa
    13021 agatgatttg aggagtaagt atatggctgt aactaagctg gttctggttc gtcatggcga
    13081 aagtcagtgg aacaaagaaa accgtttcac cggttggtac gacgtggatc tgtctgagaa
    13141 aggcgtaagc gaagcaaaag cagcaggtaa gctgctgaaa gaggaaggtt acagctttga
    13201 ctttgcttac acctctgtgc tgaaacgcgc tatccatacc ctgtggaatg tgctggacga
    13261 actggatcag gcatggctgc ccgttgagaa atcctggaaa ctgaacgaac gtcactacgg
    13321 tgcgttgcag ggtctgaaca aagcagaaac cgctgaaaag tatggcgacg agcaggtgaa
    13381 acagtggcgt cgtggttttg cagtgactcc gccggaactg actaaagatg atgagcgtta
    13441 tccgggtcac gatccacgtt acgcgaagct gagcgagaaa gaactgccac tgacggaaag
    13501 cctggcgctg accattgacc gcgtgatccc ttactggaat gaaaccattc tgccgcgtat
    13561 gaagagcggt gagcgcgtaa tcatcgctgc acacggtaac tcgttacgtg cgctggtgaa
    13621 atatcttgat aacatgagcg aagaagagat tcttgagctg aatatcccga ctggtgtgcc
    13681 actggtgtat gagttcgacg agaatttcaa accgttgaaa cgctattatc tgggtaatgc
    13741 tgacgagatc gcggcgaaag cagcggccgt tgcgaaccag ggtaaagcga agtaaacgtc
    13801 attcgcttaa aaatgagaaa gccgactgca agtgagtcgg cttttttgtt tgtcgttgct
    13861 aacaatggag cacattgcct gatgctacgc tgcgcgtctt atcagggctg caaacgtaca
    13921 gtcatggcga ttcgactata aattacccgc gacgcgcttt tactgcattc gccagttgac
    13981 gtaacagagc atcggtatca tcccagccaa tgcaggcatc ggtgatgctc ttaccatagg
    14041 ccagcggttc cccactctcg aggctctgat tgccttccac cagatggctt tccaccatca
    14101 cgccaataat agccttttcg ccaccggcaa tctgctggca aacgtcagca caaacatcca
    14161 tctgcttttt gaattgtttt gacgagttag catggctgaa atcgatcatc acctgtgctg
    14221 gcaggcctgc tttgttcagc ccttctttca cttcagcaac gtgcttcgcg ctgtagttag
    14281 gctctttacc gccgcgcaga atgatatggc aatcgccgtt accgctggta ttcacaatcg
    14341 ccgaatgccc ccatttcgtt acggacagga agcagtgcgg cgcaccggcg gcattaatgg
    14401 catcgatagc cactttaatc gtaccgtcgg tgccattttt gaagccgacc ggacaagaaa
    14461 gccctgatgc cagttcgcgg tgcacctgcg attcggtggt acgtgcgcca attgcgcccc
    14521 agctcatcag gtcagcgaga tattgtgggg tgatcatatc caggaattca cccgccgctg
    14581 gcagaccgct gtcgttaata tcaagcagca atttacgggc aatacgcaga ccgtcgttga
    14641 tctggaagct gttatccata tgcggatcgt taatcagccc tttccagccc accgtggtac
    14701 gcggcttttc aaaatagacg cgcatcacga tttccagctc atcttgcagc tcttcacgca
    14761 gcgtcagcaa gcgagtggca tactctttcg ccgcgacagg atcatgaatt gagcacggac
    14821 caatcaccac caacaggcga tcatcattac ctttcaggat cttatggatc gcttttcggg
    14881 catgggcgac cgtattcgcg gcattttcag tagcggggaa tttttccagc aatgcgactg
    14941 gaggaagtaa ctctttgatt tctttgatgc gtaaatcgtc gttctgataa ttcatgtctg
    15001 ttccagtgtt gccatactta tcttagtgaa tgcaatactt ccaatctata tcttccgtca
    15061 gaatgtgtaa acggggtttt acactatgaa cggattaatc ctggaattag caagaaaatc
    15121 gccagattgt cgcgaaaaac gagatctctc ctacaatttc taactgtaac tcctttgttt
    15181 gttaattatt tcaagattct ctgctgggta tctttgcctg gctgaaatct taaaccattg
    15241 ccttatattc acctgcaaat gcactgttgg aagaggttat ccgacataac gaccataaca
    15301 ggagcatcct atgaaaatga caaaactggc cacactattt ttgactgcca ctctaagcct
    15361 tgccagcggt gccgcactgg ctgccgatag cggagcgcaa agcaataacg gccaggcaaa
    15421 cgccgcagct gatgcgggcc aggtagcccc tgatgcccgt gaaaatgtcg cgccaaataa
    15481 cgtcgacaat aacggggtaa ataccggttc tggcggcaca atgctgcatc cggatggttc
    15541 ttcaatgaac aatgacggca tgaccaaaga tgaagagcac aagaacacca tgtgcaaaga
    15601 tggtcgctgc ccggatatca ataaaaaagt ccaaaccggt gatggcatca acaatgatgt
    15661 cgataccaaa accgacggta ccacgcagta accctgcgaa atagccaata atgggagagc
    15721 ggagtctctc ccttttaatt tttcccttct aaaagcagta tgatgtcata aagttaagtg
    15781 agaataatta agggatgact cgtcatggcg cactcacact cacacacttc ctcacacctg
    15841 ccagaagata ataatgctcg tcgcttgttg tatgctttcg gcgtgactgc cgggtttatg
    15901 ctcgtagaag tcattggtgg ttttctttcc ggttctctgg cattgctggc cgatgcgggt
    15961 catatgttga ccgatactgc cgccctgctt ttcgccctac tcgcagtgca attttcccgt
    16021 cgtcctccca ccattcgcca cactttcggt tggctaagat tgaccacgct cgcggcgttt
    16081 gtgaatgcca tcgccctggt ggtgattact attttgattg tctgggaggc gatagaacgc
    16141 ttccgcactc cgcgtccggt cgagggcggc atgatgatgg caattgccgt ggctgggctg
    16201 ctggcaaata tactttcttt ctggttactt catcacggca gcgaagagaa aaacctcaac
    16261 gtgcgagcag cggcactgca tgtgctcggg gacctgctgg gttcggttgg ggctattatt
    16321 gccgcgttga ttattatctg gaccggctgg acacctgctg accccattct ctcaatactg
    16381 gtgtcgcttc tggttctgcg tagcgcgtgg cgactgttga aagatagcgt gaatgaatta
    16441 cttgaaggtg caccggtatc gctggatatc gccgaactga agcgtcgtat gtgccgggaa
    16501 atcccggaag tccgcaatgt gcaccatgtg catgtatgga tggtaggcga gaagccggtg
    16561 atgacgctgc atgtgcaggt gatcccgcca cacgatcacg atgccttgtt agatcagatc
    16621 caacactacc tgatggatca ctatcagatt gagcacgcca ctattcagat ggaatatcaa
    16681 ccttgtcatg ggccagactg ccacctgaat gaaggggtgt ctggccattc acatcatcac
    16741 cattaatggg acagcgcgcg tgaacctctt tcgcgtgcgc tgttgatcca catccggctg
    16801 ccgttgaggg caataaaggt caggatgatg tactccagag acatggcgta aacgccctga
    16861 agtgcaaaaa taacgacgct aatcacgtta ataatcaccc acaacagcca gttttccaca
    16921 tacttacgcg tcatcagaat cattgccacg atagataaca ccatcatgca tgaatcccag
    16981 aacgggaaag cgtccggctg tagttcaggc atcgcaacct gtaatcctaa tgcttgcatg
    17041 atcatgactg ccacgcgggt caaaaatgca aacaccggat tgataaatac cgtcatcaga
    17101 ccaatcgaaa caacgcaaac cgccaaccag cttagcgcct tcggcaatgg caaccagcga
    17161 attttcaact ccgcctcgtt ctgactggtt tgtcgcgacc acgcatacca gccgtaaata
    17221 ttcgcggcaa agaaaaacat ctgtaatagc aggctggcat acagctgaat ctgaaagaaa
    17281 ataatgccaa acaaggtgac gttgatcagg ccaaagaagt agttgctgat cttctccaga
    17341 ctggcaaggc caatacacag caacccggcg atcgtgccta ccgcttcgat ccatgagaga
    17401 tcataaccgc ctgcacctat tggtatatgt accaggatat tctgcacact aaaaaaatcc
    17461 atctttcccc caaagcgtaa cgttacgcct tattattcgt tatccacgta gtgtagccgc
    17521 aaaatccagc atacgattga gcggcaccag cgccctctct cgcagccttt catcaacatg
    17581 aacctcgtga ttgcttcctt cctgttctaa tgcctctgcg atggcctgaa ggccattcat
    17641 ggccatccac ggacaatgcg cgcagctgcg gcaggttgca ccctcacctg cggttggtgc
    17701 ttccagtaac tctttaactg gcaccgcctg ctgcattttg tagaaaatac cccgatcggt
    17761 tgccacaata agcctctgat gtggcaatgt tttcgcagca gcgatcagtt gactggtgga
    17821 accgaccgca tccgccatct ctacaatagc ttgcggtgat tctggatgca ccagtatggc
    17881 agcatccggg tattcttctt gcaagcgggt taacgcctga gtcttaaact catcatgcac
    17941 aatgcaggca ccctgccagc ataatatgtc tgcgcccgtc tgtttttgca cgtaacgccc
    18001 cagatgtttg tcgggtgccc agatgatttt ttcacccaaa ctatccagat gatcaataag
    18061 ttcgacggca atgcttgaag ttaccaccca atctgcgcgc gcttttaccg cagcagaagt
    18121 gttggcgtag acgacgacag tacgatcggg atgggcatcg caaaatgcgt taaattcttc
    18181 aacagagcag ccgagatcca gtgaacattc agcctgaagt gtcggcatca gaattgtttt
    18241 ttccggactg agaattttgg cggtttctcc cataaatctc accccagcga ctaacaacgt
    18301 agaagcggga tgctttgcac cgaagcgcgc catttccaga gaatcagaaa tacagccacc
    18361 ggtttcttct gccagttgtt gaatttcggg atcggtatag tagtgggcaa ccatcaccgc
    18421 attacgttct tttagcagac gttttatctt ctcgcggtaa tacgcttttt catcaatgct
    18481 taacggcgtc ggcttcgggg gaaaaggata aatcgccgtg tctggatcaa acatcacgct
    18541 catcttacca tctcgtttta caggcttaac gttaaaaccg acatgagcgt aatattcgct
    18601 gttatcggtg aaatttgttt tatatgctaa acaagatagt cgaatggtgg ggaaaagtca
    18661 ctcgaattac gcctaattcc accgacgtag agttgcttgc aaagtaagct ctgaagatgt
    18721 catcaggtta caaagtgaaa ttgaggatgc ctgaatagca aaaaagcgcc tttagggcgc
    18781 ttttttacat tggtgggtcg tgcaggattc gaacctgcga ccaattgatt aaaagtcaac
    18841 tgctctacca actgagctaa cgacccgagt ggtgggtgat gacgggatcg aaccgccgac
    18901 cccctccttg taagggaggt gctctcccag ctgagctaat cacccgatac tgcgctgaat
    18961 actacaaact acttccaccg aacccaccgt aattagtggt gggtcgtgca ggattcgaac
    19021 ctgcgaccaa ttgattaaaa gtcaactgct ctaccaactg agctaacgac ccggtggtgg
    19081 gtgatgacgg gatcgaaccg ccgaccccct ccttgtaagg gaggtgctct cccagctgag
    19141 ctaatcaccc gatactacgc tggatactac aactaactag gttagtggtg ggtcgtgcag
    19201 gatgactcga cttcgtctcg cccttcgggc cgttgctgac gcaacgttat ccttcacgtt
    19261 ttactaccgc ttccacctta gcgattggtg ggtcgtgcag gattcgaacc tgcgaccaat
    19321 tgattaaaag tcaactgctc taccaactga gctaacgacc cactttttcg ttgctttcgg
    19381 tttgtttgat atcccgtggc aacggcggca tatattactg atttcagact tgagcgcaac
    19441 aaaaatttcg atgcagatca ctcaactgct tatgattcgc acgacacgac cagaaataat
    19501 gcgacttctg gtcgtgtgtt atgcattaca tcgcgttcag acgtttttgc gcctgtttag
    19561 cgccatcagt accagggtat ttactgataa cctgctggta tacggctttc gcttttgcgg
    19621 tgtcaccttt gtcctgcatg atgacgccga ctttaaacat cgcatctgca gccttcggtg
    19681 acttcggata gtttttcact accgaagcaa aataatacgc cgcatcatct tttttaccct
    19741 tgttgtagtt taactgaccc agccaataat tggcgtttgg caggtaagtt gaatcagggt
    19801 aatttttgat gaaattctga aatgccacta ttgcttcatc ctggcgggat ttatcctgca
    19861 ccagcgcaat tgctgcattg taatccgtgt ttgcatcgcc gcttttcacc ggcgcgccag
    19921 cattcgcagt accagcatca gctgtcggcg tcgttgatgc cgccgcaccg ctttgatcac
    19981 cgctggttga ttgcgccgct gcaccaccgc tgctgaggct atcgatctgc aacaggatct
    20041 gcttttgccg ctccacgacc tgattcagtt gatactgatt ttcctgaatc tgaccgcgca
    20101 gggaatcaat atcggattga ttatcagaga gttgttgctg gagttgggtt aaaagctggc
    20161 tgtgagcatt tgaaatacgc tcaagttgag tgacgcggtc ttcgaccgag cctgagccga
    20221 cactactgat tggtgcctga gcaaaagcgg cccagggggc cgctatacca accagtaacg
    20281 acagactcaa tagttgatgt ctgaagttac tgctcatgca attctcttag taaaccagta
    20341 ccgcacgacg gtttttggag tatgccgctt cgtcatgacc cagtactgca ggtttttctt
    20401 taccgtaaga aacgatggag atctggtctg cagaaacgcc tttaccctgc aggtacatct
    20461 taacggcgtt cgcacgacgt tcacccaggg agatgttgta ttccggagta ccacgttcgt
    20521 ccgcgtgacc ttctacggtg actttgtaag acgggttgct acgcaggaag tttgcatgtg
    20581 catccagcat ttgagcgaag tcagaacgga tatcgtactt gtccagatcg aagtaaacga
    20641 tgttgttctg ctgcagctgt tgcatttgca gacgagcctg ctcttcggaa gacatgttgc
    20701 cgttgccgcc gttcgcatcc ataccagtgc cggcacccag catgccttcg ctgccgtcat
    20761 tgctggcgtt cttattggaa gaacatgccg caattgccat aacaggcaga gcaatcatca
    20821 gccctttcag cactttgttc agttgcattt caatgattcc tttactattc aattaattat
    20881 tatcacagat acggcgacca ggcagggaat ttgacctgtc catcagttgc cggaagacgc
    20941 gctttgaaac gcccatctgt agaaaccaaa ttcagcacgg atcccatccc ctgagaagag
    21001 ctgtagatta ccatagtgcc gttaggtgcc agacttggcg tttcatccag gaacgtggac
    21061 gacagaactt gtacgcctcc cgttgccaga tcttgtttgg caatgtgctg ctgcccacca
    21121 ttggagctga ccattaccat aaatttaccg tcgctgctga catccgcatc ctggttctgc
    21181 gaaccttccc aggtaatacg ttgtggcgca ccgccgttga tattcacttt ataaacctgt
    21241 ggacgaccgg cctggtcaga agtaaatgcc aggttctggc tgtccgggaa ccaggtcggt
    21301 tcggtattgt tactgcgacc atccgtcacc tggcggatct gaccagaagc caaatccatt
    21361 acgtacaggt tcagactacc ggttttcgac aaggcgaatg ccagtttgct gccgtctggc
    21421 gagaatgcag gtgcaccgtt gtgacgcggg aatgaagcca cctgacgtac agcgccattt
    21481 gccagcgttt ggatgaccag cgcggaacga ccgctttcga aggtcacata agccagttta
    21541 gaaccgtctg gtgaccacgc cggagacatc agcggctgcg gtgaacggtg aacgacaaac
    21601 tggttgtaac cgtcatagtc agatacgcac agttcatacg ggaactgacc gccgttggtc
    21661 tgaacaacgt aggcaatacg ggtacggaac gcacctttaa tgccggtcag tttttcaaac
    21721 acttcatcac tggcggtatg accagcataa cgcagccact gcttgttcac tttgtacgag
    21781 ttctgagcaa gtacagtacc cggtgcgccg ccagtgtcaa caagttgata agcaacattg
    21841 taagaaccat ccggattcgg agtgacctga ccgacaacta cagcgtcaat acccagcgcg
    21901 gaccatgcag ctggttgtac ttcctgcgca ctacccggct gctgtggcag acgagcgcga
    21961 tctaacggat taaatttacc gctgttacgc aagtcggcag caacgatgcc gccaatatct
    22021 tcaggtgccg caccagggcc cgcccactgg aaaggaacaa ctccaatagg acgaccggaa
    22081 tctacaccgc tgtcgatcac aatgcggact tcagcatgca gaactgatgc ccacagtatg
    22141 agaaaaccaa atgctactcg taatgcctgc ttcatcatat ctcccttatc tggaccgatg
    22201 gcccacgata atttagcaga atgttaacaa actaaaatac acaaaactac cagaaccccg
    22261 tggcaaccgg tgcctgatgt tgaccgtcgg aacagtcaac atcgcgatta cggtttgaag
    22321 tccaatggcg cgtttttgaa cacttcatat actgcctggc ttggtggttt cgggatcttc
    22381 gcaagtttag ctgctgccaa cgcagcctga caaagtgcgg gatcgccacc ttcaggtttg
    22441 atatccagta acataccatc gggagccagt tttatgcgca gcgtacaggt tttgcctgca
    22501 taggacgatg cgtcatagaa cttactttcg atagcagatt taatctgccc ggcatagtta
    22561 ttgatatcgg cccctgatgc gccattgttt ttagtattac cactcccggc tggcgaagca
    22621 ttgttccctt tcgcccctcc ccccgttttc ggtgcattct taccagagct tagctcaccg
    22681 aaaatatcat ctgcctctgc ggcagctttt gctgcagcgg ctttttcagc tgctgctttt
    22741 gcagctgccg cttttttgtc tgcggcggct ttttctgccg ctgctttctt atcagctgct
    22801 gccttttcag cagccgcttt ttctgctgct tttttgtcgg ctgcagcttt ctctgctgcc
    22861 gctttcttat cagctgcagc cttttcagca gccgctttct tctcagcttc tgctttggct
    22921 ttttcagcag cttcagttgc cgctttcttt cttgcttcag ctgcagctgc ttctgccgct
    22981 tccgctttct tcttcagtgc cgccgcggct gcctcggctt ttttctgcgc ttcggctgcg
    23041 gctttggcgg cttctgcttc tgcttttttc ttcgcgtctg cagccgcttt cttcgcagct
    23101 tcttccgcag ctttagcatc cgcttcggcc ttcgctttag catctgccgc cgctttcgct
    23161 gccgcttctt ccgcttgctt ctgctttaac tcggcctgtt ttgcggcttc ttcagcctgc
    23221 tttttctgct cctgagcagc taaccgctct ttctcaagtt gcttcaggcg ttcctgttca
    23281 gccgcttgtt tctcacgcag ctcttccgca gcctgctgtt ccttcatctt gcgctgctca
    23341 tcagaacgct tcgcgcttga ttcctggctt tgcatgcgtt tgtactgctc aactaccgca
    23401 cctgaatcaa ccatgacagc gtcgatggat gaaccaccgc cgcctccggc tgaagcttct
    23461 atattctcat cgaacgaact ccagatcagc gccgcaaata agatgacatg cagcactgct
    23521 gaaataatta tcgcccgctt gagcttgtcg ttttgttcgg ttgcctttga cactctcggt
    23581 ttccaaaaac tgttcgcctg ttacccgctc tctttcaagc aagggaaacg cagatgttta
    23641 gataggctgc gtcattaaac caaccgattt cacacccgca ctatgtaaca agttcagtgc
    23701 tttaattatt tcatcgtaag gcacatcttt tgcgccaccg atcagaaaga ccgttttcgg
    23761 gttggccttg aaacggctgg acacttccgc caccacctgc tctggtggta aacgctccag
    23821 gcgatctttc tcaaccacca cggtgtactg accaatacca gacacttcaa caatcactgg
    23881 cggattatcg ttactgctca ccgcctgtga ttcagtagcg tctggcagat cgacctccac
    23941 gctctgggtg atgatgggcg ctgtcgccat aaagatcagc aacagcacca gcagtacgtc
    24001 cagcaacggt acaatgttga tttcggactt gagatcgcga cgacctcgtc cacgcgctct
    24061 ggccatggct taccccttgt tgctctcgct aacggtaaac gcctggcggt gcagaatcgc
    24121 ggtaaactct tccataaagt tgtcgtaatt cagttccagt ttgtttacgc gctggttgag
    24181 acggttataa gccattaccg ccggaattgc ggcgaacaga ccaattgccg tcgcaatcaa
    24241 cgcttctgcg ataccgggcg caaccatttg cagtgttgct tgttttaccg ccccgagggc
    24301 gataaaggcg tgcatgatcc cccagaccgt accaaacaga ccaatatacg ggctgatgga
    24361 gccaaccgta ccgaggaacg gaatgtgcgt ttccagattt tcaagttcac ggttcatgga
    24421 gatacgcata gcacgcgacg ccccttccac tacggcttcc ggcgcatggc tattggcacg
    24481 atgcaggcgc acaaactctt tgaacccgct gtaaaagatt tgttccgaac cagtcagatt
    24541 atcccgtttc ccctgactct cttgatagag gcgagagagt tcgattccag accagaattt
    24601 atcttcaaac gcttcggctt cacgcgccgc tgcgttaaga atacgggtcc gctggataat
    24661 aatggcccag gatgcgattg aaaaaccaat caaaatcaac atgataagtt taaccagaag
    24721 gctagccttc aggaacaaat caaggatatt catgtcagtc actgcttaaa ctccgcgaca
    24781 atagacttgg gaagcgcacg aggcttcatt ttgagtgggt caacgcaaac aaccagaacc
    24841 tctgcttcat tcagcaaagt attctcggcg ttgacaatac gttgcgtgaa aaccaaagag
    24901 gtgccacgca ttgatgttat ttcagtctgt atttcgagca tatcgtcgag ccgcgcaggt
    24961 gcgtaatatt ccaccgtcat tttacgtacc acaaaggcaa cgcgttcagc catcagcgcc
    25021 tgttgactga agtgatgatg acgcagcatc tctgtgcgtg ctctttcata aaaagcgacg
    25081 taactggcgt ggtacaccac accaccggca tcggtatctt catagtagac gcgaaccggc
    25141 catcgaaaca gcgttgtatt cactttacat cccggtaatg caacaaaagt tagagctttt
    25201 aaacgctgct actatacgcg cgcgattgag gtttgggaat gagggcaagt taagggagcg
    25261 taaaaattta tgggcctgga gcggcccata attttttaaa gagaattctg atttaaaaga
    25321 agaagaaaat cagcccgaca atgagaacaa tatcggcaag caatgggcaa aaaatccctt
    25381 gccaaagaac cttctgcgga cgaaagccca cgccgtgaat cacaccagca cagacggccc
    25441 acatcagcaa taaaccatgc cagatttcca gttcactggt cttcgcggcg aaacgtgatg
    25501 ggtcccaaaa catacatcct gctaacagaa gcgccatcac gaaggaaagc gcccgcaggg
    25561 ggcgcttgtc cattaccgca tacaaggttg cgataatctt actcatcaga tgtcttcttg
    25621 accggctttg cctgattcga cgtgctcaag cgccagcgcg gtgattaccc caaacgaaca
    25681 ggcaagaagc gttcccagaa tccatgcgaa ataccacatt tttagctcct tacttagtac
    25741 agagagtggg tgttacgttc aatatcttct ttggtgatac gaccgaacat tttccagtaa
    25801 caccaggcgg tgtagagcag aatgatcggt accagaacca ccgcaaccca ggtcatgacg
    25861 ttaagcgtca gctggctgga agttgcatcc cacattgtca gacttgcgtt catcatggtg
    25921 ctggacggca tcacaaacgg gaacattgcg ataccggctg tcaggatgat gcaggccagc
    25981 gtcagggagg agaacacaaa cgcccacgcc gctttatcca tacgtgcagt caggatggtc
    26041 agcagcggca gaaccacacc cagtgccgga atagcccaca gaattggcgt gttgttgaag
    26101 ttaaccagcc atgcgccagc ttcacgaacc acttctttat tcagtgggtt agaggctgcg
    26161 taatggtcca ttgtcgattt cacgacataa ccatcgatac cgtacatcac ccatacgcca
    26221 gccagtgcga aacagaccag tgtcaccagt gccgccacct gagccgttgc acgggtacgc
    26281 aggtgcagtt cgcccacggt acgcatttgc agataggttg cgccctgagt aatgatcatc
    26341 cctacgctca ccacgcctgc cagcaggccg aacgggttaa gcaggtggaa gaagttaccg
    26401 gtgtagtaca gacgcagata ttcatcaacg ttgaacggta cgccctgcaa caggttaccg
    26461 aacgctacac caatcaccaa cggcggaacg aagctaccaa tgaagatgcc ccagtcccac
    26521 atgttacgcc agcgggtttc ttcaatcttg gagcggtagt caaaaccgac cggacggaag
    26581 aacaaagacg ccagcacgag gatcatcgcc acatagaagc cggagaacgc agcggcatag
    26641 accatcggcc aggcagcaaa gagtgcgccg cccgcggtga taagccatac ctggttaccg
    26701 tcccagtgtg gtgcaatgga gttaatcata attcgacgct cggtgtcgtt acgaccgagg
    26761 aaacgagtga gcatgcccac ccccatgtcg aaaccgtcag tgactgcaaa accaatcagc
    26821 agaacgccaa ccagcagcca ccagataaaa cgcaatactt cataatcgat catttgacga
    26881 ctcctgtctt agcgtgccgg ctgagtagtc gtggaagact gctcaaagtg atagcgaccg
    26941 gttttcaggc tgcttgggcc gaggcgtgca aatttgaaca ttaagaacaa ttctgccacc
    27001 aggaacaggg tatacaggcc gcaaatcagc accattgaga agatgagatc gcctgcggtc
    27061 agtgacgagt tcgccacagc tgtcggcagc acttcaccga tagcccacgg ttggcgacca
    27121 tattcagcca cgaaccagcc cgcttctaca gcaatccacg gcagcggaat accgtacagc
    27181 gcggcgcgca gcagccattt tttctcgcca atgcggttgc ggatgacact ccagaaagag
    27241 agcgcgatga ttgccagcag caggaagcca cacgccacca tgatacggaa cgcgaagtac
    27301 agcggcgcta cacgcggaat ggagtctttg gttgcctgtt gaatctgcgc ttcagtcgca
    27361 tcagccacgt ttggcgtata gcgtttcagc agcagaccgt aaccgaggtc tttcttcatg
    27421 ctattgaact ggtcacgaac cgcctggtcg gtagaaccag aacgcagttg ctcgagcaga
    27481 gagtacgcct tcatcccgtt acgaatgcgt tcttcatgct gcaccatcag ttctttcagg
    27541 ccgataactg gagtatccac ggaacgcgtt gcaatgatgc ccagcgcgta agggatctgg
    27601 atcgcaaatt tgttcgtctc ctcttcctga tcaggaatgc cgaacagagt aaaggcagca
    27661 ggcgcaggtt gcgtttccca ctcggcttca atagcagcca gtttggtttt ctgcacgtcg
    27721 cccatttcgt aaccagattc atcacccaga acaataacag acagaacggc agccataccg
    27781 aagctggcag cgatagcaaa agagcgttta gcgaaggcga agtcacgacc tttcagcata
    27841 taccatgcgc tgataccgag gatgaacatc gcgccagtca cataaccaga cgctacagtg
    27901 tgaacgaatt tcacctgagc aaccgggtta agcaccagct cggagaagct caccatctcc
    27961 atacgcatag tttcaaagtt gaaatcggac gcgattgggt tttgcatcca gccgttcgca
    28021 accagaatcc acagtgcgga caggtttgaa ccgagcgcca ccagccaggt gacacacata
    28081 tgctgaactt tacccagacg atcccaaccg aagaagaaca gacctacaaa ggtggattcg
    28141 aggaagaagg ccatcagacc ttcgattgcc agcggcgcac cgaagatatc ccctacatag
    28201 tgggaatagt aagaccagtt agtcccgaac tggaactcca tggtcagacc ggtagccaca
    28261 cccagagcga agttgatacc aaacaacttg ccccagaact tggtcatatc tttataaatc
    28321 tgtttgccgg aaaggacgta gaccgtttcc ataatggcca gcaggaacgc cataccgagc
    28381 gtcagtggca caaaaaggaa gtggtacatc gcggtcaagg caaactgtaa gcgcgacagt
    28441 tcgactatat ctaacatcat gactccttgc tcatcgcatg aagactccga gagtgaaccc
    28501 cgttagaaag ggtcacacgc atgccccaat acaaaattat ttgctcgcct ctatcgttgc
    28561 catttcatca aaagaggaaa agacaatgat aaaaaagtta caaatgtgtt aatgaataaa
    28621 ccaaattgat cccgtaatta tattacgccg caaaatcctt acaataaaca ggtttttatt
    28681 gagcaaattt gcatttttcg acagtgatca atttatagcg aaattgtcac gatctcacca
    28741 atttgatcta c
//
LOCUS       DAZFLD010000040        28686 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.040, whole genome shotgun sequence.
ACCESSION   DAZFLD010000040 DAZFLD010000000
VERSION     DAZFLD010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28686
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.040"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            22..111
                     /gene="cydH"
                     /locus_tag="ACHKBL_001466"
                     /gene_synonym="ynhF"
     CDS             22..111
                     /gene="cydH"
                     /locus_tag="ACHKBL_001466"
                     /gene_synonym="ynhF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I accessory subunit CydH"
                     /protein_id="HFO5818351.1"
                     /translation="MSTDLKFSLVTTIIVLGLIVVVGLTAALH"
     gene            277..1446
                     /gene="ydhP"
                     /locus_tag="ACHKBL_001467"
     CDS             277..1446
                     /gene="ydhP"
                     /locus_tag="ACHKBL_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO5818352.1"
                     /translation="MKINYPLLALAIGAFGIGTTEFSPMGLLPVIARGVDVSIPAAGM
                     LISAYAVGVMVGAPLMTLLLSHRARRSALIFLMAIFTLGNVLSAIAPDYMTLMLSRIL
                     TSLNHGAFFGLGSVVAASVVPKHKQASAVATMFMGLTLANIGGVPAATWLGETIGWRM
                     SFLATAGLGVISMVSLFFSLPKGGAGARPEVKKELAVLMRPQVLSALLTTVLGAGAMF
                     TLYTYISPVLQSITHATPVFVTAMLVLIGVGFSIGNYLGGKLADRSVNGTLKGFLLLL
                     MVIMLAIPFLARNEFGAAISMVVWGAATFAVVPPLQMRVMRVASEAPGLSSSVNIGAF
                     NLGNALGAAAGGAVISAGLGYSFVPVMGAIVAGLALLLVFMSARKQPETVCVANS"
     gene            complement(1592..2173)
                     /gene="sodB"
                     /locus_tag="ACHKBL_001468"
     CDS             complement(1592..2173)
                     /gene="sodB"
                     /locus_tag="ACHKBL_001468"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HFO5818353.1"
                     /translation="MSFELPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK
                     GTAFEGKSLEEIIRSSEGGVFNNAAQVWNHTFYWNCLAPNAGGEPTGKVAEAIAASFG
                     SFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDATPLLTVDVWE
                     HAYYIDYRNARPGYLEHFWALVNWEFVAKNLAA"
     gene            complement(2301..3128)
                     /gene="mepH"
                     /locus_tag="ACHKBL_001469"
     CDS             complement(2301..3128)
                     /gene="mepH"
                     /locus_tag="ACHKBL_001469"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-endopeptidase MepH"
                     /protein_id="HFO5818354.1"
                     /translation="MARINRISITLCALLFTTLPLTPMAHASKQARESSATTHITKKA
                     DKKKSTATTKKTQKASQKTAKKAASKSTTKSKTASSVKKSSITASKNAKTRSKHAVNK
                     TASANFTEKCTKRKGYKSHCVKVKNAASGTLADAHKAKVQKATKVAMNKLMQQIGKPY
                     RWGGSSPRTGFDCSGLVYYAYKDLVKIRIPRTANEMYHLRDAAPIERSELKNGDLVFF
                     RTQGRGTADHVGVYVGNGKFIQSPRTGQEIQITSLSEDYWQRHYVGARRVMTPKTLR"
     gene            3463..3810
                     /gene="grxD"
                     /locus_tag="ACHKBL_001470"
     CDS             3463..3810
                     /gene="grxD"
                     /locus_tag="ACHKBL_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monothiol glutaredoxin 4"
                     /protein_id="HFO5818355.1"
                     /translation="MSTTIEKIQRQIAENPILLYMKGSPKLPSCGFSAQAVQALAACG
                     ERFAYVDILQNPDIRAELPKYANWPTFPQLWVDGELVGGCDIVIEMYQRGELQQLIKE
                     TAAKYKSEEPDAE"
     gene            complement(3861..8477)
                     /gene="lhr"
                     /locus_tag="ACHKBL_001471"
     CDS             complement(3861..8477)
                     /gene="lhr"
                     /locus_tag="ACHKBL_001471"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HFO5818356.1"
                     /translation="MADNLNPSSPLPDVFSPATRDWFLRAFKQPTAVQSQTWHVAARG
                     EHALVIAPTGSGKTLAAFLYALDQLFREGGEDTREAHKRKTSRILYISPIKALGTDVQ
                     RNLQIPLQGIADERRRRGETEVNLRVGIRTGDTPAQERSKLTRNPPDILITTPESLYL
                     MLTSRARETLRGVETVIIDEVHAVAGSKRGAHLALSMERLDTLLHTSAQRIGLSATVR
                     SASDVAAFLGGDRPVTVVNPPAMRHPQIRIVVPVANMDDVSSVASGTGEDSHAGREGS
                     IWPYIETGILDEVLRHRSTIVFTNSRGLAEKLTARLNELYAARLQRSPSIAVDAAHFE
                     STSGATSNRVQSSDIFIARSHHGSVSKEQRAITEQALKSGELRCVVATSSLELGIDMG
                     AVDLVIQVATPLSVASGLQRIGRAGHQVGGVSKGLFFPRTRRDLVDSAVIVECMFAGR
                     LENLTPPHNPLDVLAQQTVAAAAMDALQADEWYSRVRRAAPWKDLPRRVFDVTLDMLS
                     GRYPSGDFSAFRPKLVWNRETGILTARPGAQLLAVTSGGTIPDRGMYSVLLPEGEEQA
                     GSRRVGELDEEMVYESRVNDIITLGATSWRIQQITRDQVIVTPAPGRSARLPFWRGEG
                     NGRPAELGEMIGDFLHLLADGAFFSGTIPPWLAEENTIANIQGLIDEQRNATGIVPGS
                     RHLVLERCRDEIGDWRIILHSPYGRRVHEPWALAIAGRIHALWGADASVVASDDGIVA
                     RIPDTDGKLPDAAIFLFEPEKLLQIVREAVGSSALFAARFRECAARTLLMPGRTPGHR
                     TPLWQQRLRASQLLEIAQGYPDFPVILETLRECLQDVYDLPALERLMRRLNGGEIQIS
                     DVTTTTPSPFAASLLFGYVAEFMYQSDAPLAERRASVLSLDSELLRNLLGQVDPGELL
                     DPQVIRQVEEELQRLAPGRRAKGEEGLFDLLRELGPMTVEDLAQRHTGSSEEIASYLE
                     NLLAVKRIFPTMISGQERLACMDDAARLRDALGVRLPESLPEIYLHRVSYPLRDLFLR
                     YLRAHALVTSEQLAHEFSLGIAIVEEQLQQLREQGLVMNLQQDIWVSDEVFRRLRLRS
                     LQAAREATRPVAATTYARLLLERQGVLPATDGSPALFASTSPGVYEGVDGVMRVIEQL
                     AGVGLPASLWESQILPARVRDYSPEMLDELLATGAVIWSGQKKLGEDDGLVALHLQEY
                     AAESFTPAEANQANSSALQQAIVTVLADGGAWFAQQISQRIRDKIGESVDPSAMQEAL
                     WALVWQGVITSDIWAPLRALTRSSTNTRTSTRRSHRARRGRPVYAQPVSPLVSYNTPK
                     LAGRWSLLQVEPLNDTERMLALAENMLDRYGIISRQAVIAENIPGGFPSMQTLCRSME
                     DSGRIMRGRFVEGLGGAQFAERLTIDRLRDLATQATQTRHYTPVALSANDPANAWGNL
                     LPWPAHPATLIPTRRAGALVVVSGGKLLLYLAQGGKKMLVWQEKEELLAPEIFHALTT
                     ALRREPRLRFTLTEVNDLPVRQTPMFTLLREAGFSSSPQGLDWG"
     gene            complement(8570..9217)
                     /gene="rnt"
                     /locus_tag="ACHKBL_001472"
     CDS             complement(8570..9217)
                     /gene="rnt"
                     /locus_tag="ACHKBL_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016896 - RNA exonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HFO5818357.1"
                     /translation="MSDNAQLTGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITL
                     KMDEQGWLMPDTTLHFHVEPFVGANLQPEALAFNGIDPNDPDRGAVSEYEALHEIFKV
                     VRKGIKASGCNRAIMVAHNANFDHSFMMAAAERASLKRNPFHPFATFDTAALAGLALG
                     QTVLSKACQTAGMDFDSTQAHSALYDTERTAVLFCEIVNRWKRLGGWPLPAAEEV"
     gene            complement(9320..9727)
                     /gene="gloA"
                     /locus_tag="ACHKBL_001473"
     CDS             complement(9320..9727)
                     /gene="gloA"
                     /locus_tag="ACHKBL_001473"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HFO5818358.1"
                     /translation="MRLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFV
                     GYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGP
                     VKGGTTVIAFVEDPDGYKIELIEEKDAGRGLGN"
     gene            complement(9808..10905)
                     /gene="nemA"
                     /locus_tag="ACHKBL_001474"
     CDS             complement(9808..10905)
                     /gene="nemA"
                     /locus_tag="ACHKBL_001474"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylmaleimide reductase"
                     /protein_id="HFO5818359.1"
                     /translation="MSSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDIPTPLMAE
                     YYRQRASAGLIISEATQISAQAKGYAGAPGIHSPEQIAAWKKITAGVHAENGHMAVQL
                     WHTGRISHASLQPGGQAPVAPSALSAGTRTSLRDENGQAIRVETSMPRALELEEIPGI
                     VNDFRQAIANAREAGFDLVELHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEV
                     VDAGIEEWGADRIGIRVSPIGTFQNTDNGPNEEADALYLIEQLGKRGIAYLHMSEPDW
                     AGGEPYTDAFREKVRARFHGPIIGAGAYTVEKAETLIGKGLIDAVAFGRDWIANPDLV
                     ARLQRKAELNPQRAESFYGGGAEGYTDYPTL"
     gene            complement(10942..11541)
                     /gene="nemR"
                     /locus_tag="ACHKBL_001475"
     CDS             complement(10942..11541)
                     /gene="nemR"
                     /locus_tag="ACHKBL_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310384.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NemR"
                     /protein_id="HFO5818360.1"
                     /translation="MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKTAEVPKGS
                     FYHYFRSKEAFGVAMLERHYATYHQRLTELLQSGEGNYRDRILAYYQQTLNQFCQHGT
                     ISGCLTVKLSAEVCDLSEDMRSAMDKGARGVIALLSQALENGRDSHCLTFCGEPLQQA
                     QVLYALWLGANLQAKISRSFEPLENALAHVKTIIATPAV"
     gene            11644..11883
                     /gene="ydhL"
                     /locus_tag="ACHKBL_001476"
     CDS             11644..11883
                     /gene="ydhL"
                     /locus_tag="ACHKBL_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HFO5818361.1"
                     /translation="MAEQLEFFPVQSPCRGICQSDERGFCRGCFRSRDERFNWNKMSD
                     GEKQEVLRLCRQRLMRKLRANKPASSDEPEQPSLF"
     gene            11932..12828
                     /gene="ydhF"
                     /locus_tag="ACHKBL_001477"
     CDS             11932..12828
                     /gene="ydhF"
                     /locus_tag="ACHKBL_001477"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HFO5818362.1"
                     /translation="MVQRITIAPQGPEFSRFVMGYWRLMDWNMSARQLVSFIEEHLDL
                     GVTTVDHADIYGGYQCEAAFGEALKLAPHLRERMEIVSKCGIATTAREENVIGHYITD
                     RDHIIKSAEQSLINLATDYLDLLLIHRPDPLMDADEVADAFKHLHQSGKVRHFGVSNF
                     TPAQFALLQSRLPFTLATNQVEISPVHQPLLLDGTLDQLQQLRVRPMAWSCLGGGRLF
                     NDDYFQPLRDELAVVAEELNAGSIEQVVYAWVLRLPSQPLPIIGSGKIERVRAAVEAE
                     TLKMTRQQWFRIRKAALGYDVP"
     gene            12909..13430
                     /gene="sodC"
                     /locus_tag="ACHKBL_001478"
     CDS             12909..13430
                     /gene="sodC"
                     /locus_tag="ACHKBL_001478"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC"
                     /protein_id="HFO5818363.1"
                     /translation="MKRFSLAILALVVATGAQAASEKVEMNLVTSQGVGQSIGSVTIT
                     ETDKGLEFSPDLKALPPGEHGFHIHAKGSCQPATKDGKASAAESAGGHLDPQNTGKHE
                     GPEGAGHLGDLPALVVNNDGKATDAVIAPRLKSLDEVKDKALMVHVGGDNMSDQPKPL
                     GGGGERYACGVIK"
     gene            complement(13431..15443)
                     /gene="ydhK"
                     /locus_tag="ACHKBL_001479"
     CDS             complement(13431..15443)
                     /gene="ydhK"
                     /locus_tag="ACHKBL_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFO5818364.1"
                     /translation="MNASSWSLRNLPWFRATLAQWRYALRNTIAMCLALTVAYYLNLD
                     EPYWAMTSAAVVSFPTVGGVISKSLGRIAGSLLGAIAALLLAGHTLNEPWFFLLSMAA
                     WLGFCTWACAHFTNNVAYAFQLAGYTAAIIAFPMVNITEASQLWDIAQARVCEVIVGI
                     LCGGMMMMILPSSSDATALLTALKNMHARLLEHASLLWQPETTDAIRAAHEGVIGQIL
                     TMNLLRIQAFWSHYRFRQQNARLNALLHQQLRMTSVISSLRRMLLNWPSPPCATREIL
                     EQLLTALASSQTDVYTVARIIAPLRPTNVADYRHVAFWQRLRYFCRLYLQSSQELQHL
                     QSGVDDHTRLPRTSGLARHTDNAEAMWSGLRTFCTLMMIGAWSIASQWDAGSNALTLA
                     AISCVLYSAVAAPFKSLSLLMRTLVLLSLFSFVVKFGLMVQISDLWQFLLFLFPLLAT
                     MQLLKLQMPKFAALWGQLIVFMGSFIAVTNPPVYDFADFLNDNLAKIVGVALAWLAFA
                     ILRPGSDARKSRRHIRALRRDFVDQLSRHPTLSESEFESLTYHHVSQLSNSQDALARR
                     WLLRWGVVLLNCSHVVWQLRDWESRSDPLSRVRDNCISLLRGVMSERGVQQKSLAATL
                     EELQRICDSLARHHQPAARELAAIVWRLYCSLSQLEQAPPQGTLAS"
     gene            complement(15443..16300)
                     /gene="ydhJ"
                     /locus_tag="ACHKBL_001480"
     CDS             complement(15443..16300)
                     /gene="ydhJ"
                     /locus_tag="ACHKBL_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416161.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HFO5818365.1"
                     /translation="MSIKTIKYFSTIIVAVVAVLAGWWLWNYYMQSPWTRDGKIRAEQ
                     VSITPQVSGRIVELNIKDNQLVNAGDLLLTIDKTPFQIAELNAQAQLAKAQSDLAKAN
                     NEANRRRHLSQNFISAEELDTANLNVKAMQASVDAAQATLKQAQWQLAQTEIRAPVSG
                     WVTNLTTRIGDYADTGKPLFALVDSHSFYVIGYFEETKLRHIREGAPAQITLYSDNKT
                     LQGHVSSIGRAIYDQSVESDSNLIPDVKPNVPWVRLAQRVPVRFALDKVPGDVTLVSG
                     TTCSIAVGQ"
     gene            complement(16303..16539)
                     /gene="ydhI"
                     /locus_tag="ACHKBL_001481"
     CDS             complement(16303..16539)
                     /gene="ydhI"
                     /locus_tag="ACHKBL_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HFO5818366.1"
                     /translation="MKFMLNATGLPLQDLVFGASVYFPPFFKAFAFGFVIWLVVHRLL
                     RGWIYAGDIWHPLLMDLSLFAICVCLSLAILIAW"
     gene            16740..17174
                     /gene="slyA"
                     /locus_tag="ACHKBL_001482"
     CDS             16740..17174
                     /gene="slyA"
                     /locus_tag="ACHKBL_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SlyA"
                     /protein_id="HFO5818367.1"
                     /translation="MESPLGSDLARLVRIWRALIDHRLKPLELTQTHWVTLHNIHQLP
                     PDQSQIQLAKAIGIEQPSLVRTLDQLEEKGLISRQTCASDRRAKRIKLTEKAEPLISE
                     MEAVINKTRAEILHGISAEELEQLIKLIAKLEHNIIELQAKG"
     gene            complement(17221..17688)
                     /gene="slyB"
                     /locus_tag="ACHKBL_001483"
     CDS             complement(17221..17688)
                     /gene="slyB"
                     /locus_tag="ACHKBL_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein SlyB"
                     /protein_id="HFO5818368.1"
                     /translation="MIKRVLVVSMVGLSLVGCVNNDTLSGDVYTASEAKQVQNVSYGT
                     IVNVRPVQIQGGDDSNVIGAIGGAVLGGFLGNTVGGGTGRSLATAAGAVAGGVAGQGV
                     QSAMNKTQGVELEIRKDDGNTIMVVQKQGNTRFSPGQRVVLASNGSQVTVSPR"
     gene            17962..19071
                     /gene="anmK"
                     /locus_tag="ACHKBL_001484"
     CDS             17962..19071
                     /gene="anmK"
                     /locus_tag="ACHKBL_001484"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HFO5818369.1"
                     /translation="MKSGRFIGVMSGTSLDGVDVVLATIDEHRVAQLASLSWPIPVSL
                     KQAVLDICQGQQLILSQFGQLDTQLGRLFADAVNALLKEQNLQARDIVAIGCHGQTVW
                     HEPTGVAPHTLQIGDNNQIVARTGITVVGDFRRRDIALGGQGAPLVPAFHHALLAHPT
                     ERRMVLNIGGIANLSLLIPGQPVGGYDTGPGNMLMDAWIWRQAGKPYDKDAEWARAGK
                     VILPLLQNMLSDPYFSQPAPKSTGREYFNYGWLERHLRHFPGVDPRDVQATLAELTAV
                     TISEQVLLSGGCERLMVCGGGSRNPLLMARLAALLPGTEVTTTDAVGISGDDMEALAF
                     AWLAWRTLAGLPGNLPSVTGASQETVLGAIFPANP"
     gene            19169..19498
                     /gene="mliC"
                     /locus_tag="ACHKBL_001485"
     CDS             19169..19498
                     /gene="mliC"
                     /locus_tag="ACHKBL_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-type lysozyme inhibitor"
                     /protein_id="HFO5818370.1"
                     /translation="MSMKKLLLICLPVLLTGCSAFNQLFERMQTDTLEYQCDEKPLTV
                     KLNNPRQEVSFVYDNQLLHLKQGISASGARYTDGIYVFWSKGEEATVYKRDRIVLNNC
                     QLQNPQR"
     gene            19557..20213
                     /gene="pdxH"
                     /locus_tag="ACHKBL_001486"
     CDS             19557..20213
                     /gene="pdxH"
                     /locus_tag="ACHKBL_001486"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HFO5818371.1"
                     /translation="MSDNDELQQIAHLRREYTKGGLRRRDLPADPLTLFERWLSQACE
                     AKLADPTAMVVATVDEHGQPYQRIVLLKHYDEKGMVFYTNLGSRKAHQIENNPRVSLL
                     FPWHTLERQVMVIGKAERLSTLEVMKYFHSRPRDSQIGAWVSKQSSRISARGILESKF
                     LELKQKFQQGEVPLPSFWGGFRVSLEQIEFWQGGEHRLHDRFLYQRENDAWKIDRLAP
                     "
     gene            20342..21616
                     /gene="tyrS"
                     /locus_tag="ACHKBL_001487"
     CDS             20342..21616
                     /gene="tyrS"
                     /locus_tag="ACHKBL_001487"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HFO5818372.1"
                     /translation="MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTA
                     DSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVD
                     KIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
                     LNREDQGISFTEFSYNLLQGYDFACLNKQYGVVLQIGGSDQWGNITSGIDLTRRLHQN
                     QVFGLTVPLITKADGTKFGKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFT
                     FMSIEEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLQAAKRITECLFSGSLSA
                     LSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQS
                     DPEYFFKEEDRLFGRFTLLRRGKKNYCLICWK"
     gene            21675..22538
                     /gene="pdxY"
                     /locus_tag="ACHKBL_001488"
     CDS             21675..22538
                     /gene="pdxY"
                     /locus_tag="ACHKBL_001488"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase"
                     /protein_id="HFO5818373.1"
                     /translation="MMKNILAIQSHVVYGHAGNSAAEFPMRRLGANVWPLNTVQFSNH
                     TQYGKWTGCVMPPSHLTEIVQGIAAIDKLHTCDAVLSGYLGSAEQGEHILGIVRQVKA
                     ANPQAKYFCDPVMGHPEKGCIVAPGVAEFHVRHGLPASDIIAPNLVELEILCEHAVNN
                     VEEAVLAARELIAQGPQIVLVKHLARAGYSRDRFEMLLVTADEAWHISRPLVDFGMRQ
                     PVGVGDVTSGLLLVKLLQGATLQEALEHVTAAVYEIMVTTKAMQEYELQVVAAQDRIA
                     KPEHYFSATKL"
     gene            complement(22582..23187)
                     /gene="gstA"
                     /locus_tag="ACHKBL_001489"
     CDS             complement(22582..23187)
                     /gene="gstA"
                     /locus_tag="ACHKBL_001489"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HFO5818374.1"
                     /translation="MKLFYKPGACSLASHITLRESGKDFTLVSVDLMKKRLENGDDYF
                     AVNPKGQVPALLLDDGTLLTEGVAIMQYLADSVPDRQLLAPVNSISRYKTIEWLNYIA
                     TELHKGFTPLFRPDTPEEYKSTVRAQLEKKLQYVNEALKDEHWICGQRFTIADAYLFT
                     VLRWAYAVKLNLEGLEHIAAFMQRMAERPEVQDALSAEGLK"
     gene            complement(23293..24795)
                     /gene="dtpA"
                     /locus_tag="ACHKBL_001490"
     CDS             complement(23293..24795)
                     /gene="dtpA"
                     /locus_tag="ACHKBL_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpA"
                     /protein_id="HFO5818375.1"
                     /translation="MSTANQKPTESVSLNAFKQPKAFYLIFSIELWERFGYYGLQGIM
                     AVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWLGDKVLGTKRVIMLGAIVLAIGY
                     ALVAWSGHDAGIVYMGMAAIAVGNGLFKANPSSLLSTCYEKNDPRLDGAFTMYYMSVN
                     IGSFFSMIATPWLAAKYGWSVAFALSVVGLLITIVNFAFCQRWVKQYGSKPDFEPINY
                     RNLLLTIIGVVALIAIATWLLHNQEVARMALGVVAFGIVVIFGKEAFAMKGAARRKMI
                     VAFILMLEAIIFFVLYSQMPTSLNFFAIRNVEHTILGLAVEPEQYQALNPFWIIIGSP
                     ILAAIYNKMGDTLPMPTKFAIGMVMCSGAFLILPLGAKFASDAGIVSVSWLVASYGLQ
                     SIGELMISGLGLAMVAQLVPQRLMGFIMGSWFLTTAGANLIGGYVAGMMAVPDNVTDP
                     LMSLEVYGRVFLQIGVATAVIAVLMLLTAPKLHRMTQDDAADKAAKAAVA"
     gene            complement(25406..26041)
                     /gene="nth"
                     /locus_tag="ACHKBL_001491"
     CDS             complement(25406..26041)
                     /gene="nth"
                     /locus_tag="ACHKBL_001491"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HFO5818376.1"
                     /translation="MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATD
                     VSVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNG
                     EVPEDRAALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVE
                     EKLLKVVPAEFKVDCHHWLILHGRYTCIARKPRCGSCIIEDLCEYKEKVDI"
     gene            complement(26041..26736)
                     /gene="rsxE"
                     /locus_tag="ACHKBL_001492"
     CDS             complement(26041..26736)
                     /gene="rsxE"
                     /locus_tag="ACHKBL_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HFO5818377.1"
                     /translation="MSEIKDVIVQGLWKNNSALVQLLGLCPLLAVTSTATNALGLGLA
                     TTLVLTLTNLTISTLRHWTPAEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFI
                     PLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGMGATCAMFVLGSLREIIGNGTLFDG
                     ADALLGSWAKVLRVEIFHTDSPFLLAMLPPGAFIGLGLMLAGKYLIDERMKKRRAEAA
                     AERALPNGETGNV"
     gene            complement(26740..27360)
                     /gene="rsxG"
                     /locus_tag="ACHKBL_001493"
     CDS             complement(26740..27360)
                     /gene="rsxG"
                     /locus_tag="ACHKBL_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HFO5818378.1"
                     /translation="MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQKA
                     LFDQVLPAERYNNALAQSCYLVTAPELGKGEHRVYIAKQDDKPVAAVLETTAPDGYSG
                     AIQLLVGADFNGTVLGTRVTEHHETPGLGDKIELRLSDWITHFAGKKISGADDANWAV
                     KKDGGDFDQFTGATITPRAVVNAVKRAGLYAQTLPAQLSQLPACGE"
     gene            complement(27364..28422)
                     /gene="rsxD"
                     /locus_tag="ACHKBL_001494"
     CDS             complement(27364..28422)
                     /gene="rsxD"
                     /locus_tag="ACHKBL_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HFO5818379.1"
                     /translation="MVFRIASSPYTHNQRQTSRIMLLVLLAAVPGIAAQLWFFGWGTL
                     VQILLASVSALLAEALVLKLRKQSVAATLKDNSALLTGLLLAVSIPPLAPWWMVVLGT
                     VFAVIIAKQLYGGLGQNPFNPAMIGYVVLLISFPVQMTSWLPPHEIAVNIPGFIDAIQ
                     VIFSGHTTSGGDMNTLRLGIDGISQATPLDTFKTSVRAGHSVEQIMQYPIYSGMLAGV
                     GWQWVNLAWLAGGVWLLWQKAIRWHIPLSFLVTLALCATLGWLFSPETLASPQIHLLS
                     GATMLGAFFILTDPVTASTTNRGRLMFGALAGLLVWLIRSFGGYPDGVAFAVLLANIT
                     VPLIDYYTRPRVYGHRKG"
     gene            complement(28423..>28686)
                     /locus_tag="ACHKBL_001495"
     CDS             complement(28423..>28686)
                     /locus_tag="ACHKBL_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001439589.1"
                     /note="part of membrane-bound complex thought to be
                     involved in electron transport to nitrogen; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HFO5818380.1"
                     /translation="AVEAAIARAKARKLEQQQANAEPEEQVDPRKAAVEAAIARAKAR
                     KLEQQQANAEPEEQIDPRKAAVAAAIARVQAKKAAQQKVVNED"
BASE COUNT         7008 a         7273 c         7518 g         6887 t
ORIGIN      
        1 aataaaaaag gagaaaactt gatgagcacc gaccttaaat tttcactggt aacaacgatt
       61 atcgtcctcg gtttgatcgt ggtcgtgggt ttgactgccg cgctgcactg atttcctctc
      121 gagggagcgc gttctccctc gcatctcctt cctgtttgtt agcaaaatgg caaaagtgtt
      181 ttctcttttt gttattttca tttttttgtg attgctgtca tgctttcgcc actttaatga
      241 atatcgcgta atgacgcgaa gaggtggaga taaggcatga aaattaacta tccgttgctg
      301 gcgctggcga ttggcgcgtt tggtatcggg acaacggagt tctcgccaat gggcttgttg
      361 cccgtcattg cgcgcggtgt ggatgtctcg attcccgctg ccggaatgtt aatcagtgct
      421 tatgcagttg gcgtaatggt tggcgcgccg ctgatgacgc ttctactttc tcatcgtgcc
      481 cgccgcagtg cgttgatttt cctgatggcg attttcacgc ttggcaacgt actttccgcc
      541 atcgcgccgg attatatgac cctgatgctt tcacgcattt tgaccagcct gaatcacggg
      601 gcattttttg gtttgggttc agtcgtggcc gcaagcgtgg tgccaaaaca taaacaggcc
      661 agcgcagttg ccactatgtt tatggggtta accctggcaa atatcggtgg cgtgccggcg
      721 gcgacctggt tgggtgaaac catcggctgg cggatgtcat ttctggcaac ggcggggctg
      781 ggagtgattt caatggtaag tctgttcttc tcattaccta aaggtggtgc aggggcacga
      841 cctgaagtga aaaaagagct ggcggtatta atgcgtccgc aggtgctgtc tgcattgctg
      901 acgacggtac tgggagctgg tgcaatgttt accctctaca cctatatctc tccggtactg
      961 caaagtatta cccacgcaac accggtgttc gtcacagcaa tgctggtgct gattggtgtc
     1021 ggattctcta tcggtaacta tctcggcggc aaactggcag atcgttcagt taacggcacg
     1081 ttgaaaggct ttttgttgct gctgatggtg attatgctgg caatcccgtt cctggcccgc
     1141 aatgagttcg gcgcagctat tagcatggtg gtgtggggcg ctgcaacctt tgcggtcgta
     1201 ccgccgttac agatgcgcgt gatgcgtgtc gccagtgaag cgccaggtct gtcttcatca
     1261 gtcaatattg gtgcctttaa tcttggaaat gcgctgggag cagctgctgg tggtgcggta
     1321 atttccgctg ggctgggata cagctttgtg ccggtgatgg gggcgattgt cgcgggactg
     1381 gcattattgc tggtgtttat gtcagccaga aaacaacctg aaacagtttg cgttgccaac
     1441 agctaattaa acaaaaacgc agaaggataa ccttctgcgt tacagttgct ggcaacgtgc
     1501 gatttatctt gccgaatgcg ctgttaacgc ctcatccgtg ttggcatggc gcgcgaagcg
     1561 catcaggtaa tgttgcattt gccatcagtt attatgcagc gagatttttc gctacgaatt
     1621 cccagttcac cagcgcccag aagtgctcca gatagccagg acgtgcattg cgatagtcga
     1681 tgtaataagc gtgttcccag acatcaacgg tcagcagcgg agttgcatcg gtggtcagtg
     1741 gagtacccgc gttagaggtt gaaacgatag ccagtttgcc atcgctgttt ttcaccagcc
     1801 aggtccagcc agaaccaaag tttttgatcg ctgcatcagt aaactgagct ttgaaatcgg
     1861 caaagctgcc aaaagatgcg gcgatagctt cagcgacttt tccagtcggt tcgccacccg
     1921 cgttcggtgc caggcagttc cagtagaaag tatggttcca gacctgagct gcgttgttga
     1981 atacgccacc ttcagagctg cgaataatct cttccagtga tttaccttca aacgcggtac
     2041 ctttaatcag gttgttcagg ttagtgacat aagtctgatg atgcttgcca tagtgatact
     2101 cgatggtttc cgcagaaatg tgcggtgcca gagcatcttt agcatatggt agtgcaggta
     2161 attcgaatga cattgctact ctcctttatt attaatttgc atacgtacaa tagccttatt
     2221 gtgcgtatgg atagggtagc aaattaaaag gtgagacaaa agatgaactt accctgttgc
     2281 cgtaacaaca gggtaaagtt ttagcgaagt gtttttgggg tcattacccg acgagcgcca
     2341 acatagtggc gctgccagta gtcttcactg agagaagtga tttgaatttc ttggcccgtg
     2401 cgcggtgact gaataaattt gccgttgccg acatacacgc cgacatgatc ggctgtaccg
     2461 cgtccctgag tacggaagaa gaccaggtcg ccgtttttca gttcactacg ttcgataggt
     2521 gctgcatcac gcaggtgata catttcattc gccgtacgcg gaatacgaat tttcaccaaa
     2581 tctttataag cgtaataaac caggccgctg caatcaaaac cggtacgcgg tgagctgccg
     2641 ccccaacgat atggcttacc aatttgttgc atcagtttat tcattgccac tttcgtggct
     2701 ttttgcacct tcgctttgtg cgcgtcggca agggttcctg acgcggcatt tttgactttc
     2761 acacaatgcg atttatagcc cttacgtttg gtacactttt cggtaaagtt ggctgaggcc
     2821 gttttattga cggcatgttt gctgcgagtt ttggcgtttt tagaagcggt aatggaagat
     2881 tttttaacgg aagaagcggt tttgcttttg gtcgtacttt tactggcggc ttttttcgct
     2941 gttttctggc tggctttctg ggtctttttg gtggttgccg tgcttttctt tttatctgct
     3001 tttttggtga tatgagtagt agcagaactc tccctggctt gctttgaagc atgggccata
     3061 ggcgttaaag gcagggtggt aaaaagtaaa gcacagagcg tgatcgaaat acggtttatc
     3121 cgcgccactg agcgtccctc tgttaaggca ggtctatcat gcctgcgtct gatttacaaa
     3181 acttgatgat tctaatgcat agaagaagag aaagttaata gcttttattg ttaaaaaata
     3241 cgtttcgttg gcgagcgcaa aaaaatgcag cagtgaaagc taaaaatggt tataaatacg
     3301 ccagtttgcc gtgcgtcagg gaagaaacga atatttgaaa tgcaacaaat agagcaaagg
     3361 gataaaatag caaaagcgtg atgaaaatgt tattttccga tgccgcactg acccgctaca
     3421 atgtcagatt actgccgtaa agaagtttga ggaagcaaga caatgagcac cactatcgaa
     3481 aaaatccaac gccagattgc tgaaaacccg atcctgctgt acatgaaagg ttcaccgaaa
     3541 ctgccgagct gcggtttctc tgcccaggca gtccaggcgc ttgccgcatg tggcgaacgt
     3601 tttgcctatg ttgatatcct gcagaatccg gacattcgtg cggaactgcc gaaatatgct
     3661 aactggccga ccttcccgca actgtgggtt gacggcgagc tggtcggcgg ttgtgatatc
     3721 gtgatcgaaa tgtatcagcg tggcgaactg cagcagctga tcaaagaaac tgccgctaaa
     3781 tacaagtctg aagagccgga cgctgaataa tccgtcctgc aatactgacg gatgcgaacg
     3841 ggcattcgtc agtcctttct ctatccccaa tccagccctt gtggcgaact tgaaaatccc
     3901 gcctcacgca gcagcgtaaa catcggcgtt tgccggaccg gtaggtcatt cacttctgtt
     3961 agcgtaaagc gcagccgtgg ttcgcgacgc agtgcggtag tcagcgcgtg gaaaatctct
     4021 ggggcgagta attcctcttt ttcctgccag accagcattt ttttgccacc ttgcgccaga
     4081 tagagtaaca atttgccgcc agaaacgacc accagcgcac ccgcccgacg cgttggtatc
     4141 agcgttgccg gatgtgcagg ccagggcaga agatttcccc atgcattagc cggatcgttg
     4201 gcagagagcg ccactggtgt atagtggcgc gtttgcgtgg cttgtgtcgc cagatcgcgc
     4261 aatcggtcaa tagtcagacg ttcagcgaat tgcgcgccac ccagaccttc tacaaaacga
     4321 cctcgcataa ttcgcccgga gtcttccata cttcgacaaa gcgtttgcat cgatggaaac
     4381 ccgccaggga tattttcggc tatcaccgcc tgacgactga tgatgccgta gcggtcgagc
     4441 atattttccg ccagcgccag catcctttcg gtatcgttta gtggctccac ctgcaataac
     4501 gaccagcgtc cagcaagctt tggtgtgttg taagatacca gcggcgagac gggttgcgca
     4561 tagacaggac gtccacgacg agcccggtga ctgcggcgag ttgaggtgcg tgtgttggta
     4621 ctgctgcggg tgagggcgcg taacggtgcc caaatgtcgc tggtgatgac gccttgccag
     4681 accagcgccc ataacgcctc ttgcatggct gagggatcaa ccgattcgcc gattttatcg
     4741 cgtatccgtt ggctgatttg ttgtgcaaac caggctcctc cgtcagccag aaccgtgact
     4801 atcgcttgct gcagcgccga actattcgcc tgattcgctt cggcgggagt gaacgattct
     4861 gcggcatatt cctgtagatg cagtgccacc agaccgtcat cttcacccag ctttttttgc
     4921 cccgaccaga taaccgcacc ggttgccagt aattcatcga gcatttctgg cgaatagtcg
     4981 cgtacgcggg caggcaggat ctggctttcc cagagtgagg cgggtaaacc gactccggca
     5041 agctgttcga tcacccgcat gacgccatcc acgccctcat aaacgcctgg cgatgttgag
     5101 gcaaagagtg ccgggctacc atcggtggcg ggtaatacgc cctgacgttc cagcagcaat
     5161 cgcgcatagg tcgtggccgc aacgggacgc gtcgcttctc tggcggcttg cagcgagcgc
     5221 aaacgcagac gacgaaatac ttcatcgctc acccagatgt cttgttgcag attcatcacc
     5281 agcccctgtt cgcgcagttg ctgaagttgc tcttcgacaa tggcaatacc gagactaaac
     5341 tcatgagcca gttgttcaga cgtgaccaga gcatgagccc ggagatagcg cagaaagagg
     5401 tcgcgaagcg ggtaactgac tcggtgcaaa taaatctctg gcagtgactc tggtagtcgt
     5461 acgccgagag catcgcgcag cctggcggca tcatccatac aggcaagacg ctcctgcccg
     5521 ctaatcatgg tcgggaagat tcgttttact gcaagaagat tttccagata cgacgcaatc
     5581 tcttcactgc tgcctgtatg tcgttgcgcc aggtcttcaa cggtcattgg ccccagttcg
     5641 cgcagcaggt cgaacaatcc ttcttcacct ttcgctcttc tgccaggagc cagtcgttgc
     5701 aactcttctt ccacctggcg aatgacctgt gggtcgagta attcccccgg atcgacctgt
     5761 ccgagtagat tgcgcagtaa ctcgctgtcc agcgacagta cggatgcccg gcgctctgcc
     5821 agcggggcgt cgctctggta cataaattcc gcgacatagc cgaacaataa actggcggcg
     5881 aaaggcgaag gcgtagtggt cgttacatcg gatatttgaa tttcgccacc gttcaggcga
     5941 cgcatcaaac gttccagcgc gggaagatca taaacatctt gcagacattc gcgtagggtt
     6001 tcgagaatga ccggaaaatc tggatatccc tgagcgattt ccagcaactg actggcgcgc
     6061 agccgttgtt gccagagcgg ggtgcgatgg cccggcgtgc ggcccggcat taataatgtc
     6121 cgcgcggcgc attcgcgaaa acgggcggcg aaaagtgccg agctgcctac cgcctcgcgg
     6181 acaatttgca gcaacttttc tggttcaaac aaaaaaatcg cggcatcagg caatttacca
     6241 tcggtgtcag gaatgcgcgc aacaatgccg tcatcactgg caaccacaga cgcgtcagcg
     6301 ccccatagtg catgtattcg cccggcaatc gccagcgccc agggttcatg cacccgtctt
     6361 ccatagggag agtgcaaaat aatacgccag tcaccaattt catcacggca ccgttcgagg
     6421 accagatgac gactccccgg aacgatgccc gtcgcgttgc gctgctcgtc aatcaacccc
     6481 tgaatattgg cgatcgtatt ttcttctgcc aaccacgggg gaatagtccc ggaaaagaac
     6541 gcgccatccg ccagtaaatg gagaaaatcg ccgatcatct cgcctaattc tgccggacgt
     6601 ccgttacctt caccacgcca gaagggaagg cgggcagaac gacctggagc aggagtcaca
     6661 atcacctgat cgcgggtgat ttgctggatc cgccatgagg tagcgccgag agtgataatg
     6721 tcgttcaccc gcgactcata taccatctcc tcatccagtt cacccacccg ccgcgaaccg
     6781 gcctgttctt caccttcggg taataacacg ctatacatgc cacgatccgg aatggtgcca
     6841 ccgctggtaa ccgccaacag ctgagcgcca ggtcgggcgg tcaatatccc ggtctccctg
     6901 ttccagacca gttttggtcg aaaagcagaa aaatcgccag agggatagcg cccggaaagc
     6961 atatccagcg tgacgtcaaa aacacgtctt ggcagatctt tccacggtgc ggcacggcgt
     7021 acgcgggagt accattcgtc tgcctgtaat gcatccatcg ccgcggcggc aacggtttgc
     7081 tgcgcaagga cgtcgagtgg attatgcggc ggtgtcaggt tttccagcct gcctgcgaac
     7141 atacactcta caatgactgc ggaatcgact aagtcacgcc tggttcgggg gaaaaaaagc
     7201 cctttagata caccgccaac ctgatgtccg gcgcgaccaa tgcgttgtaa cccactggca
     7261 acagaaagcg gcgttgccac ctgaatcacc agatccaccg cgcccatatc aatccccagt
     7321 tcgagactgg aggttgcgac cacacagcgt aattcacccg atttcaacgc ctgttcggtg
     7381 attgctcgtt gttctttaga gacggagccg tggtgtgagc gggcaataaa aatgtcgcta
     7441 ctttgtacac ggttagaggt tgcgccggag gtcgactcga aatgggccgc atcaacggcg
     7501 atagatgggg aacgctgtaa gcgtgcggcg taaagctcat ttaatcgtgc cgtcagtttt
     7561 tccgccagcc cgcgtgaatt agtaaagaca atggtcgagc gatggcgcaa aacttcatca
     7621 aggatacccg tttcaatata tggccagatg gagccttccc ggccggcatg gctgtcttcg
     7681 ccggtgccgc tggcgaccga tgagacatca tccatattgg cgaccggtac gacaatccgt
     7741 atctgcggat ggcgcattgc gggcgggttg actaccgtaa ccgggcgatc gccaccaaga
     7801 aatgctgcca catcgctggc tgaacgcaca gtggcagaaa ggccaattcg ctgtgctgag
     7861 gtgtggagca gcgtatcgag ccgctccata cttaacgcca gatgcgcacc acgtttactg
     7921 cccgctaccg cgtggacttc atcaataatt accgtttcga cgccgcgtag cgtttcgcgc
     7981 gcgcgggagg tcagcatcag atagagtgat tcgggtgtgg tgatcagaat atccggcgga
     8041 ttacgggtga gtttgctgcg ttcctgtgca ggcgtatcgc cagtacggat ccccacgcga
     8101 agattgactt ccgtttcgcc gcgccgccgc cgttcatcgg caataccctg taacgggatc
     8161 tgcaagttgc gctgaacgtc ggtgcccaga gctttgatcg gtgaaatata gaggatgcgt
     8221 gaggttttac gcttatgcgc ctcgcgggta tcttcgccgc cttcgcggaa gagctgatcg
     8281 agggcgtaga gaaatgctgc cagcgttttc ccggagccag tcggtgcaat caccagcgca
     8341 tgttcgcctc gcgccgccac atgccaggtt tgcgactgga cagcggtcgg ctgtttaaag
     8401 gcgcgaagaa accagtcgcg ggtcgccggt gaaaacacgt ccggcagggg cgatgaaggg
     8461 tttagattat ctgccattaa cgctattccc cgtgaatatc atgcatgatg cgtggaatag
     8521 cctcactata gatgcctgaa tcattgcatg tcatcaggca tcgactcgat tacacctctt
     8581 cggcggcagg tagcggccag cctcccagac gtttccagcg gttgacgatt tcacaaaaca
     8641 gcacagcagt gcgttcggtg tcgtacagcg cggagtgcgc ctgggtgctg tcgaagtcca
     8701 tgccagcggt ctggcaagcc tttgacaata cggtttgtcc gagtgccagc ccggccagtg
     8761 cagcagtgtc aaaagtggcg aaagggtgga acgggttacg tttcagtgag gcgcgttctg
     8821 cggcggccat cataaagctg tgatcgaaat tggcgttgtg cgccaccata atggcgcggt
     8881 tacaaccgct cgctttaata cctttacgca caactttaaa aatttcgtgc agcgcctcgt
     8941 attcgctgac cgcgccgcga tcgggatcgt tcgggtcaat gccgttgaag gcgagggcct
     9001 ctggctgcaa atttgcgccg acaaatggtt cgacgtggaa atgtaatgtg gtgtccggca
     9061 tcagccagcc ttgttcatcc attttcaggg tgatggcggc aatctcaagc agcgcatcgg
     9121 ttttggcgtt aaatccagct gtttcaacat cgatcacaac aggataaaaa ccacgaaaac
     9181 ggtcgcacag accggtaagt tgagcgttat cggacatctg attctcttaa tacggataaa
     9241 ttgcagcgcg cattatgaca aatattactt gtcgatgcag taaagatgcg ggcgcgatga
     9301 gttcacgccc ggcaggagat tagttgccca gaccgcgacc ggcgtctttc tcttcgatta
     9361 actcaatttt gtaaccgtcc ggatcttcca caaacgcgat aaccgtagtg ccgcctttta
     9421 ccggacccgc ttcacgggtg acgttaccgc cgttctgacg gattttttcg cacgcttcag
     9481 cggcgttatc tacgctaagc gcgatgtgac cataagcagt gccgagttcg tatttatcca
     9541 cgccccagtt gtaggtcagt tcaatcaccg cttcttcggt ttccgggccg tagccaacaa
     9601 acgccagtga gtatttgtat tccgggtttt cgctggtacg cagcagtttc atgcccagta
     9661 ctttggtata aaaatcgatg gagcgttgca gatcgccaac gcgcagcatg gtatgaagaa
     9721 gacgcatctt tatatcctca aaatggctaa cagattcaaa atgttgtttt agtatagcgg
     9781 cggctttgcg ccgctcgcaa tgttggatta cagcgtcggg taatcagtgt agccttccgc
     9841 gccgccaccg taaaaacttt cggcacgctg tgggttaagc tcagctttgc gttgcaagcg
     9901 ggcgaccaga tccgggttcg caatccagtc acgaccaaat gccaccgcat caattaaccc
     9961 tttgccgatc agcgtttcgg ctttttctac cgtgtatgca cctgcgccga taatcggacc
    10021 gtggaaacgg gcgcgtactt tttcgcggaa cgcatcagta tacggttcac cccccgccca
    10081 atctggttct gacatatgca gataagcaat gccgcgttta cccagttgtt ctatcagata
    10141 cagtgcatcg gcttcttcat tcgggccgtt atctgtgttc tggaaagtac cgattggcga
    10201 aacgcgaatg ccaatgcgat cggcacccca ttcttcaatc ccggcatcga ccacttccag
    10261 caccagacgt gcgcgatttt ccacgctgcc gccgtactga tcggtacgat ggtttgaaga
    10321 aggagaaagg aactgatgca gcaaataacc atgagcagag tggagctcta ccagatcaaa
    10381 accggcttca cgcgcgttag caatggcctg acggaaatca ttgacgatac ccggaatctc
    10441 ttccagttca agcgcacgcg gcatggatgt ttcaacacgg atcgcctgac cattttcatc
    10501 gcgcagagaa gtacgtgttc ccgcgctaag tgcggaaggc gctaccggtg cctgaccgcc
    10561 aggttgcagg ctggcgtgag aaatgcgtcc ggtgtgccac agctgtacgg ccatatgacc
    10621 attttcagca tgaacgccag cggtaatttt tttccatgcg gcaatttgtt ccggactatg
    10681 gatgccaggt gcaccagcat acccttttgc ctgggcagaa atttgcgtgg cttcgctaat
    10741 aatcaaaccg gcactggcac gttggcgata gtattccgcc atcaacgggg tagggatgtc
    10801 acccggttca atactgcgca gacgcgtcag cggtgccata aaaatacggt ttgccgccgt
    10861 gatcgcgccc actttcagtg gggaatacag tttttcagat gacataaagg ctcccggtcg
    10921 tctggtgata aaaaatgcct gctaaacggc aggcgtcgca ataatggttt ttacatgtgc
    10981 cagcgcgttt tccagtggct cgaaactgcg cgaaattttg gcctgcagat tcgcacccag
    11041 ccatagtgcg taaagcactt gtgcctgttg cagcggttcg ccacaaaagg ttaaacaatg
    11101 gctatcacgg ccattttcca gcgcctgcga gagcagggcg atcacgccgc gagcgccttt
    11161 atccatcgcg ctacgcatat cttctgacag atcgcacact tcggcagaga gttttactgt
    11221 caggcaacca ctgatggttc catgttggca aaactggttc agtgtttgct ggtaataagc
    11281 cagtatgcgg tcgcggtagt taccttcgcc ggattgcagc aactcagtca gtcgttggtg
    11341 atatgtggcg taatggcgct caagcatggc aacgccaaac gcttctttag agcgaaagta
    11401 gtgatagaag gaccctttcg gcacttcagc ggtttttagt aattcgctta gccccatccc
    11461 ggtgaatcca cgttgcaggc aaagttgctc gcccgtcgcc aggagatgtt cgcgagtatc
    11521 atgttcggtg tgtttgttca tgcgttggag tgtagtagac cagtcggtct agtgcaagca
    11581 aagttgctac tttgggcatt aacagcttca ataatggtaa taatcttatt tgaggagggc
    11641 gaagtggcgg agcaattaga gttctttccc gttcagagcc cgtgccgggg aatttgccag
    11701 tctgacgaac gcgggttttg tcgcggctgt tttcgtagtc gggatgagcg ttttaactgg
    11761 aataaaatga gcgatggcga aaaacaagag gtgctgcgcc tttgtcggca gcgattaatg
    11821 cgcaaattac gcgcaaataa gccagcgtca tcagacgaac cggagcaacc atcactcttt
    11881 tgagcctaat cttgcgtata ctcctctcat atctgataag aggaagcgat tatggttcag
    11941 cgtattacta ttgcgccgca aggcccggag ttttcccgtt ttgtgatggg ctactggcga
    12001 ttgatggact ggaatatgtc cgcccgccag ctggtcagtt ttattgaaga gcatctggat
    12061 ctcggcgtga ccaccgtgga ccatgctgat atttatggtg gctatcagtg cgaagcggcg
    12121 tttggcgagg cgctgaaact ggctcctcac cttcgtgaac ggatggaaat cgtcagtaaa
    12181 tgcggtatcg cgacgaccgc gcgtgaagaa aacgtcattg gtcattacat tactgaccgc
    12241 gatcacatca ttaagagcgc cgaacagtcg ctaattaatc ttgcgaccga ttatctggat
    12301 ctgctgttaa tccaccgacc agacccgtta atggatgccg atgaagtggc ggacgcgttt
    12361 aaacatctgc atcagagcgg caaagtgcgc cattttggcg tatcgaactt tacgcctgcg
    12421 caatttgccc tgttgcaatc acgtctgccg tttacccttg ccactaatca ggtggaaata
    12481 tccccggtgc atcagccgtt actgctggat ggcacgctcg accaactgca acaactgcgt
    12541 gttcgtccga tggcgtggtc ctgccttggt ggtggtcgtc tgtttaatga tgattatttc
    12601 cagccgctgc gtgatgaact ggctgtggtg gcggaggagt taaacgcggg ctcgattgaa
    12661 caggtggttt acgcctgggt attacgttta ccgtcgcagc cgctgccaat tatcggctca
    12721 ggtaaaattg agcgtgtacg ggcagctgtc gaagctgaaa cactgaaaat gacccgtcaa
    12781 caatggttcc gtatccgtaa agcggcattg ggttacgacg taccgtaagc acttttagga
    12841 atagccgccg ttcaaaaatg tgtcactggt ttacacttat tcaggaatgc acaatgaacg
    12901 gaggtcctat gaaacgtttt agtctggcca ttctggcgct ggttgttgcg accggcgcac
    12961 aagctgccag tgaaaaagtc gagatgaacc tcgtcacgtc gcaaggggtt gggcagtcaa
    13021 ttggtagcgt caccattact gaaaccgata aaggtctgga gttttcgccc gatctgaaag
    13081 cattaccccc cggtgaacat ggcttccata ttcatgccaa aggaagctgc cagccagcca
    13141 ccaaagatgg caaagccagc gccgcggaat ccgcaggcgg gcatcttgat ccacaaaata
    13201 ccggtaaaca tgaagggcca gaaggtgccg ggcatttagg cgatctgcct gcactggtcg
    13261 tcaataatga cggcaaagct accgatgccg tcatcgcacc tcgtctgaaa tcactggatg
    13321 aagtcaaaga caaagcactg atggtccacg ttggcggcga taatatgtcc gatcaaccta
    13381 aaccgctggg cggtggcggt gaacgctatg cctgtggtgt aattaagtaa ttaagaggcc
    13441 agcgtacctt gtggcggtgc ttgctcaagt tgcgaaagcg agcagtacag ccgccagaca
    13501 attgccgcca gctcgcgggc ggcaggttga tgatggcggg caaggctgtc gcaaatccgc
    13561 tgtaattctt caagtgtggc cgccagtgat ttttgctgaa cgccacgctc actcatcact
    13621 ccccgcaaca gtgaaataca gttatcccgt actcgcgata acggatcgga acgcgattcc
    13681 cagtcgcgca attgccagac aacatgagaa cagttcagca gcactacacc ccagcgtaat
    13741 aaccaacggc gagccagcgc atcctggctg ttactcaact gactgacgtg atgataagtg
    13801 agcgattcaa attcgctttc actcagtgtt ggatggcggc ttagttgatc gacaaaatcc
    13861 cggcgcagcg cgcgtatatg gcggcggctt ttgcgagcat ccgatcctgg acgcagaatg
    13921 gcgaacgcta accacgccaa cgcgacgcca acgatttttg ccagattatc gttaagaaaa
    13981 tcagcaaaat catacaccgg gggattagtg acagcgataa acgaacccat aaaaacaatc
    14041 agttgccccc acagtgcggc aaatttgggc atctgcaatt taagaagctg cattgtcgcc
    14101 agcagtggaa agagaaacag taaaaattgc cacagatcgc taatctgcac catcaggcca
    14161 aatttgacca caaagctgaa tagcgaaagt aacaccagcg tgcgcatcag cagtgacaac
    14221 gacttaaacg gtgctgcgac ggcggagtag agtacgcagc taattgctgc cagcgttaat
    14281 gcattggaac cggcatccca ttgcgaagca atactccatg cgccaatcat catcaacgta
    14341 caaaatgtac gcagtccgct ccacatagct tcggcgttat cggtatgacg agccaggccg
    14401 gatgtccgtg ggagtctggt atgatcatcc acaccgcttt gcagatgttg taattcctga
    14461 ctactttgca gataaaggcg gcaaaaataa cgtagtcgct gccagaaggc gacgtgccga
    14521 tagtcggcga cgttggtcgg gcgtagcggg gcgataatac gtgcgacggt gtaaacatct
    14581 gtttgcgaac tggcgagcgc cgtcagcagc tgttcgagaa tttctcgtgt ggcacatggc
    14641 ggtgagggcc agttgagcaa catacgtcgc aggctggaga tgacactggt catacgtaat
    14701 tgctggtgga gcagcgcatt aaggcgtgcg ttttgctggc gaaaacgata gtggctccag
    14761 aaagcctgga tacggagcaa attcatggtc agtatctgtc caatcacccc ctcatgtgct
    14821 gcacgaatgg catcggttgt ttcaggctgc cagagtaaac tggcatgttc cagtaatcgg
    14881 gcgtgcatgt ttttcaatgc ggttaaaagg gctgtagcat cggaactgct cggcaggatc
    14941 atcatcatca tgccgccgca caaaataccg acaatcacct cgcaaacgcg cgcctgagcg
    15001 atatcccaca gctggctggc ctcagtaata ttaaccatcg gaaaggcgat gatggcagct
    15061 gtgtagcccg ccagttgaaa tgcatacgcg acattattcg tgaagtgcgc acaggcccag
    15121 gtacaaaagc caagccacgc cgccatgctc aaaagaaaaa accacggctc attgagcgta
    15181 tgtccggcaa gaagcagtgc cgcaatggct ccgagcaaac tgccagcgat gcgtccgagg
    15241 cttttgctga tgacaccgcc aacggtggga aagctaacca cggcagccga ggtcatcgcc
    15301 caatagggtt catccagatt taaataatag gcaaccgtca gcgccagaca catggcaatg
    15361 gtattgcgta acgcataacg ccattgcgcc agcgtggccc tgaaccaggg caaattgcgc
    15421 aaggaccatg acgatgcgtt cattattgac ctacggcaat actacaggtg gtgcccgaca
    15481 ccagcgtgac atcgcctggg actttatcca gcgcaaagcg gacgggaact cgttgggcga
    15541 ggcgaaccca ggggacgtta ggtttcacat ccggaataag gtttgagtca ctttcaacgc
    15601 tctgatcata aatcgcccga ccgatactgg aaacgtgacc ctgtaacgtt ttgttatcac
    15661 tatagagggt aatttgtgct ggtgcacctt cgcggatatg gcgcaacttg gtttcttcaa
    15721 aataaccaat gacgtaaaac gagtggctgt cgacaagggc aaacagcggt ttcccggtgt
    15781 cagcgtaatc gccgatgcgg gtagtgaggt tagtcaccca tccactcacc ggggcgcgaa
    15841 tttccgtttg cgccagttgc cattgtgcct gtttcagcgt cgcctgtgcg gcatcaacac
    15901 tggcctgcat cgctttaaca ttgaggttag cggtatcgag ttcttcggca gaaataaaat
    15961 tttgtgagag atgacgacga cgattcgctt cgttattggc ttttgcgagg tcagattgcg
    16021 ctttcgccaa ctgagcctgt gcgttcagct cggcgatctg aaagggcgtt ttatcgatcg
    16081 tgagcagaag atcccccgcg ttaaccagtt ggttgtcttt tatattcagc tcaacaatac
    16141 gtcccgacac ctgaggggtg atagaaacct gttctgcgcg tattttcccg tcgcgtgtcc
    16201 acggagattg catgtaataa ttccacagcc accatccggc aagaaccgca actaccgcta
    16261 caatgattgt tgagaaatac ttaattgttt taattgacat agttaccacg caatcagtat
    16321 tgccagggaa aggcaaacgc aaatcgcaaa cagcgataaa tccattaaca agggatgcca
    16381 gatgtcaccg gcgtagatcc agccacgaag caggcggtgt acgacaagcc agatgacgaa
    16441 tccaaacgcg aaggctttga aaaacggagg aaagtagacg gacgcaccga acaccagatc
    16501 ttgtaagggc aatcctgttg cattgagcat aaacttcaca aagtagattc ctttacgacc
    16561 gaatatattg cgttagatta ataaatattc tttaagtgcg aaaaatttac gcgcaatttc
    16621 tgaaaaatat gtctgacggt aaccaaatgc agcaatacat ttgttttagc aatacaattg
    16681 ctgcacacta ttctaaaagc cgcataatat cttagcaagc taattataag gagatgaaat
    16741 tggaatcgcc actaggttct gatctggcac ggttggtgcg catatggcgt gctctgatag
    16801 accatcgcct gaaaccgctg gagttaacac aaacccattg ggttacgtta cacaatatcc
    16861 atcagttacc tccagaccag tcgcaaattc aactggcaaa agcgattggc atcgagcagc
    16921 catcactggt ccgtactctg gaccaactgg aagaaaaagg gttaatttcg cgtcaaactt
    16981 gtgccagcga tcgtcgggct aaacgtatta aactgacgga aaaggcagag ccgctgatca
    17041 gcgaaatgga agctgttatt aacaaaaccc gcgcggaaat tttacatggc atctccgcag
    17101 aggaactgga acaactgatt aagctcatcg caaaacttga gcataatatc attgagttac
    17161 aggccaaagg gtgaaatgaa gggggcgtgt ggtcagtaac ctgaccacac gcaaacttat
    17221 ttagcgcggg gaaacggtca cctgactgcc attgctggcc agtacgacac gttggcccgg
    17281 agagaaacga gtgttgcctt gtttctgtac caccatgatg gtattaccat cgtctttacg
    17341 aatttccagc tcgacaccct gcgttttgtt cattgcactc tgtacgccct gaccggctac
    17401 gccacctgca acagcgcctg cagcagtagc cagagaacgc ccggttccgc caccaacagt
    17461 attccccagg aaaccaccaa gaacagcacc gccaattgcg ccgataacgt tggaatcatc
    17521 accgccctga atctgtaccg gacgtacgtt aacgatggtg ccatagctga cattctgtac
    17581 ttgtttcgct tcagaagcgg tataaacatc ccctgacagg gtgtcgttat taacacaacc
    17641 gacaagagac agacctacca ttgaaacaac caatacgcgt ttaatcattg aaaaatctcc
    17701 tgttcaccat gaaacgccac gcaagcatcc ctcatggtca aagtatatgg catatgtgaa
    17761 tccatggtta catgttctac caaatatgag caaatcataa tgaaagtagg atcaaccaat
    17821 tataaacaaa acattcatga tgattattga tttgaagata atgttttact accaacggta
    17881 aaaatcggtc attccgcatg gtattcacga aaagtttatg tttgagtggt gcgcaaacgt
    17941 cagattgaaa aaggatgaac tatgaaatcg ggccgcttta ttggcgttat gtcaggcacc
    18001 agccttgatg gtgttgatgt tgtgttggcg acaattgatg aacaccgggt cgcacagctg
    18061 gcaagtttga gctggccgat cccggtgtct ctgaaacagg ctgtactgga tatttgccag
    18121 ggccagcagc ttatactttc tcagtttgga cagcttgata ctcaactcgg gcgacttttt
    18181 gctgatgcgg tcaatgcctt gcttaaggaa caaaacctgc aggcgagaga tatcgttgcg
    18241 atcggctgtc acggtcaaac cgtctggcat gaaccgacgg gcgtggcacc acacacttta
    18301 cagattggcg ataacaatca aattgtggca cgcaccggaa ttacggttgt cggtgatttt
    18361 cgccgtcgcg atattgcctt gggaggacaa ggcgcaccgc tggtacctgc gttccatcat
    18421 gcgctgctgg ctcacccaac cgagcgacga atggtgctca atattggcgg catcgccaat
    18481 ctgtcactgc tcattcctgg gcagccggtt gggggctacg ataccggtcc tggtaacatg
    18541 ctgatggatg cctggatctg gcgtcaggcc ggtaaacctt acgataaaga tgccgagtgg
    18601 gcacgggcgg gtaaagttat tctcccactg ctgcaaaata tgctcagcga cccgtatttc
    18661 tcgcaacccg caccgaaaag caccggacgc gaatacttta actacggctg gctggagcgc
    18721 catttgcgcc attttccggg tgttgatccc cgtgatgtgc aggccacact ggcagaactc
    18781 accgccgtga ccatttctga acaagttttg ttgagcggtg gctgtgaacg attgatggta
    18841 tgtggtggag gaagtcgtaa tccgctactc atggcgcgtc tggcggcatt actgccaggc
    18901 acagaagtca ccaccactga tgccgttggc attagtggcg atgacatgga agcattggct
    18961 ttcgcctggc ttgcctggcg gacgcttgcg ggattaccag gaaatctgcc ttccgtcact
    19021 ggcgcaagcc aggagacggt actcggggct attttccccg ctaacccgtg atatcagagt
    19081 taactgaatt tttcttaccg cgtctgccgt tacacttgga acgttaggga gggcgtattg
    19141 ccctccagac caggaaagtc ttcgggatat gtctatgaaa aaactgttac tgatctgttt
    19201 gccggtcctt ctcaccggct gtagcgcgtt caatcaactg tttgagcgca tgcaaaccga
    19261 tacgctggaa taccagtgtg atgaaaaacc gttgacggtc aaactgaata atccgcgcca
    19321 ggaagtcagt tttgtttacg ataatcaact actgcatctc aaacagggca tttctgcgtc
    19381 tggtgcacgt tacaccgacg ggatctatgt tttctggtcg aaaggcgagg aagcgactgt
    19441 ctataaacgc gaccgcatcg tcttgaataa ctgtcagtta caaaatccac agcgttgaga
    19501 tttttccagg ggcggcgcac aatagcgtca cccactgaca atccgtaaag aaaaccatgt
    19561 ctgataacga cgaattgcag caaatcgcgc atctgcgccg tgaatacacc aaaggcggat
    19621 tacgccgccg cgatcttccc gccgatccat taaccctttt tgaacgctgg ctctctcagg
    19681 cgtgtgaagc caaactggcg gaccctaccg cgatggtggt cgctaccgtg gatgaacatg
    19741 gtcagcctta tcagcgcatc gttttactca aacattacga cgaaaaaggc atggtgtttt
    19801 acaccaacct cggcagccgt aaagcacatc aaatcgaaaa taatccgcgc gttagcctgc
    19861 tgttcccgtg gcataccctt gagcgccagg tgatggtgat cggtaaagcg gaacgacttt
    19921 cgactctcga agtgatgaaa tattttcata gtcgcccacg tgatagccag attggtgcat
    19981 gggtttcgaa gcagtccagt cgcatttctg cccgcggtat ccttgaaagt aaattcctgg
    20041 agctgaagca gaagtttcaa cagggcgaag tgccattgcc gagtttttgg ggcggttttc
    20101 gcgtcagcct tgaacagatt gagttctggc agggcggtga gcatcgcctg catgaccgct
    20161 ttttgtacca gcgtgaaaat gatgcgtgga agattgatcg tcttgcaccc tgaaaagatg
    20221 caaaaatctt gctttaatcg ctggtactcc tgattctggc actttattct atgtctattt
    20281 cgcatctggc gaaaagtcgt gtaccggcaa aggtgcagtc gttatataca tggagatttt
    20341 gatggcaagc agtaacttga ttaaacaatt gcaagagcgg gggctggtag cccaggtgac
    20401 ggacgaggaa gcgttagcag agcgactggc gcaaggcccg atcgcgctct attgcggctt
    20461 cgatcctacc gctgacagct tgcatttggg gcatcttgtt ccattgttat gcctgaaacg
    20521 cttccagcag gcgggccaca agccggttgc gctggtaggc ggcgcgacgg gtctgattgg
    20581 cgacccgagc ttcaaagctg ctgagcgtaa gctgaacacc gaagaaactg ttcaggaatg
    20641 ggtggacaaa atccgcaagc aggttgcccc gttcctcgat ttcgactgtg gagaaaactc
    20701 tgctatcgcg gcgaacaact atgactggtt cggcaatatg aatgtgctga ccttcctgcg
    20761 cgatattggc aaacacttct ccgttaacca gatgatcaac aaagaagcgg ttaagcagcg
    20821 tctcaaccgt gaagatcagg ggatttcgtt cactgagttt tcctacaacc tgttgcaggg
    20881 ttatgacttc gcctgtctga acaaacagta cggtgtggtg ctgcaaattg gtggttctga
    20941 ccagtggggt aacatcactt ctggtatcga cctgacccgt cgtctgcatc agaatcaggt
    21001 gtttggcctg accgttccgc tgatcaccaa agcagacggc accaaatttg gtaaaactga
    21061 aggcggcgca gtctggttgg atccgaagaa aaccagcccg tacaaattct accagttctg
    21121 gatcaacaca gcggatgccg acgtttaccg cttcctgaag ttcttcacct tcatgagcat
    21181 tgaagagatc aacgccctgg aagaagaaga taaaaacagc ggtaaagcac cgcgcgccca
    21241 gtatgtgctt gcagaacagg tgacacgtct ggtgcatggt gaagaaggtt tgcaggcggc
    21301 aaaacgtatt accgaatgcc tgttcagcgg ttccttgagt gcgctgagtg aagcggactt
    21361 cgaacagctg gcgcaggacg gcgtaccgat ggttgagatg gagaagggcg cagacctgat
    21421 gcaggcgctg gtcgattctg aactgcaacc ttcccgtggt caggcgcgta aaactatcgc
    21481 ctccaatgcc atcaccatta acggtgaaaa acagtccgat cctgaatact tctttaaaga
    21541 agaagatcgt ctgtttggtc gttttacctt attgcgtcgc ggcaaaaaga attactgtct
    21601 gatttgctgg aaataatgca ttaagtggaa agggggagtg agaaatcact ccccctggtt
    21661 tttatacagg gaacatgatg aaaaatattc tcgctatcca gtctcacgtt gtttatggtc
    21721 atgcgggtaa cagtgcggca gagtttccga tgcgccgcct gggcgcgaac gtctggccgc
    21781 tgaacaccgt tcaattttct aatcacactc aatacggcaa atggactggc tgcgtgatgc
    21841 cgcccagcca tttaaccgaa attgtgcaag gcattgccgc cattgataaa ttacacacct
    21901 gtgatgccgt attaagtggc tatctgggtt cggcggagca gggtgaacat attctcggta
    21961 tcgttcgtca ggtgaaagca gcgaatccgc aggcgaaata tttttgcgat ccggtaatgg
    22021 gtcatccgga aaaaggctgt atcgttgcac cgggtgtcgc agagtttcat gtgcggcacg
    22081 gtttgcctgc cagcgatatc attgcgccga atctggttga gctggaaata ctctgtgagc
    22141 atgcggtaaa taacgtcgaa gaagcggttc tggcagcgcg tgaactcatt gcgcaagggc
    22201 cacaaattgt gttggttaaa cacctggcgc gggctggcta cagccgtgac cgttttgaaa
    22261 tgctgctggt taccgccgat gaagcctggc atatcagccg tccgctggtg gattttggta
    22321 tgcgccagcc ggtaggtgtt ggtgatgtga cgagcggttt actgctggtg aaactgcttc
    22381 agggggcaac gctgcaggag gctctggaac atgtgaccgc tgcagtatac gaaatcatgg
    22441 tgaccaccaa agcaatgcag gaatatgagc tgcaagtggt tgctgctcag gatcgtattg
    22501 ccaaaccaga acattatttc agcgcaacaa agctctgaaa tatctcattt cggcccacaa
    22561 cgacctgtgg gccgatggct attactttaa gccttccgct gacagcgcgt cttgtacttc
    22621 cggacgttca gccatacgtt gcataaatgc cgcaatgtgc tctaaccctt ccagattcag
    22681 tttcaccgcg tatgcccagc gcagaaccgt aaacagatag gcatcagcaa ttgtaaatcg
    22741 ttgcccgcag atccagtgct catccttcag tgcctcgttc acatattgca gcttcttctc
    22801 cagctgcgcg cgaactgtcg atttgtactc ttccggtgta tcagggcgaa acagaggtgt
    22861 gaaaccttta tgcagctcgg tggcgatgta attcagccat tcgatggttt tatagcggga
    22921 aatactgttt accggtgcca gcaactggcg gtcggggacg ctgtcggcaa gatactgcat
    22981 aatcgctacg ccttccgtca gcaaagtacc atcgtccagc agcaatgcag gtacctgtcc
    23041 cttagggtta acggcaaagt aatcgtcacc gttttcgaga cgttttttca ttaaatccac
    23101 actgacgagc gtaaaatcct ttccgctctc acgcagggtg atatgggaag cgagagagca
    23161 ggcacccggt ttgtagaaca atttcatcgg taactccttt tgtctgtggt ttaaagtatg
    23221 gtagtgcgtt gctgaacaaa aaaaaagccg ctaacgaaaa gttagcggct tgtaaaagag
    23281 tttccctgaa ttttacgcta cggctgcttt cgccgctttg tctgcagcgt catcctgcgt
    23341 catgcggtgc agtttcggcg cggtcagcag catcagtact gcaataacgg cagtagcgac
    23401 accaatctgc aagaatacgc gaccatagac ttccagtgac atcagtggat cggtaacgtt
    23461 atccggcaca gccatcatac ccgcaacata accaccaatc aggtttgcac cggcagtggt
    23521 caggaaccag ctacccataa tgaagcccat cagacgctgc ggaacgagtt gtgcaaccat
    23581 tgccagaccc agaccagaga tcatcagttc tccgatactc tgcaggccat agcttgcgac
    23641 cagccagctt acagacacga tacctgcgtc agatgcgaat tttgcaccca gcggcagaat
    23701 caggaacgca ccagaacaca tcaccatacc gattgcaaac ttagttggca tcggcagggt
    23761 atcgcccatc ttgttataga tagcggccag aatcgggcta ccaatgatga tccagaacgg
    23821 gttcagtgcc tgatactgct caggttctac ggccaggccc agaatggtgt gttcaacgtt
    23881 acgaatcgca aagaagttca gtgacgttgg catctggctg tacagcacga agaagataat
    23941 ggcttcgagc atcaggatga aggcaacgat cattttacga cgcgcagcac ctttcatcgc
    24001 gaaggcttct ttaccgaaga taaccacgat accaaaggca acaacgccca gcgccatacg
    24061 cgcaacttcc tgattgtgca gcagccaggt ggcgatagcg atcagtgcca caacaccaat
    24121 aatggtcagc agcaggttac ggtagttgat aggctcgaag tctggttttg aaccgtactg
    24181 tttaacccag cgttggcaga aggcgaagtt aacgatagtg atcagcaggc ctacaacgct
    24241 caacgcaaac gcaacactcc agccgtattt cgcggccagc cacggggtgg cgatcataga
    24301 gaagaaagag ccgatgttga cggacatgta gtacatggtg aatgcaccgt ccagacgcgg
    24361 gtcgtttttc tcatagcatg tagaaagcag agaagacggg ttagctttaa acaggccgtt
    24421 accgaccgca atagccgcca tacccatata aacgataccg gcgtcgtgac cagaccaggc
    24481 aaccagagca taaccaatcg ccagcacaat agcgccaagc ataattacgc gtttagtacc
    24541 cagtaccttg tcacctaacc agccgccgat agcgaccaga ccataaacca gggcactaaa
    24601 ggaagagaaa agggtgattg aatccgcttc agacataccc agttgtttaa ccaggtaaac
    24661 agccataatt ccttgtaggc cgtaataacc aaaacgttcc cataactcaa tcgagaagat
    24721 gagatagaac gccttcggtt gtttgaaagc gttcaaactg acgctttcag ttggtttttg
    24781 gtttgcagtg gacactttta cctctttttt tacatcccat attaacgggg gtgctttctc
    24841 gcgttatgcc taaaaaacat acgcgcaatt gttatagtgg gaggggaaac ggcaggtaat
    24901 gttcactatc ttgcactttc agacaagtcc tttgtaataa tctgttacat ataactgagg
    24961 cggggaattc aaccattccg ggaaatgtta tccagcgtta aatttttcaa aaaacgtaac
    25021 ggcagatttt ttcactaccc tgagaggggg atgatggctt aacggcgata tgttctgcta
    25081 tttagcgttt ttcctagtga gatagtccag tttctgaaaa atagccagtg taatgttttg
    25141 taggtcaata tttgttgcgt gtgtaaattt aaatgatttt aacctagttc ttgcaatgtt
    25201 tttacattgc cacagcatcg tgatcttgat cacgaatgaa tagaaaattt cgctattaaa
    25261 aaaacaatta acctgcattt ttggttatcc gcaaacggat tacctgatga taacgctaag
    25321 caatggcatc attgacgtta aaaagatgtt ttaaaagagg ataaagaaag gttatcaatg
    25381 gggtaatcgg tgttacccct tttcttcaga tgtcaacttt ctctttgtat tcacaaagat
    25441 cttcaataat acaagagcca cagcgaggct tgcgggcaat acaggtataa cgcccgtgca
    25501 ggatcaacca atggtggcag tcgactttaa actctgccgg aaccactttc agtagctttt
    25561 cttctacctg ttcgacgttt ttccccggcg caaattgagt acgattacaa acgcggaaaa
    25621 tgtgcgtgtc gacagcaata gtaggccagc cgaatgcagt gtttaatacg acgttggctg
    25681 ttttacgacc tacaccaggc agggcttcaa gcgcagcacg atcttccgga acctcgccat
    25741 tatgctgttc cagtaagata cggcaggttt tgatgatatt ttctgctttg ctgttataaa
    25801 gcccaatggt tttgatatag gttttcaccc cttcgacgcc cagttcaagc atcgctgcag
    25861 gtgtattagc caccgggtag agtttcgccg tcgccttatt aacactgaca tcggtcgcct
    25921 gagcggaaag cagtacggca atcagcaatt caaaaggcga actgaaatta agctcggtgg
    25981 tgggatgagg attgttctca cgcaggcgag tgaggatctc caggcgtttt gctttgttca
    26041 tcagacattc cctgtttcac cgtttggcaa tgcacgttcg gcagctgctt cagcacggcg
    26101 ctttttcatt ctttcatcaa tcaggtattt ccctgccagc atcagtccca ggccaataaa
    26161 tgcacctggt ggcagcatcg ccagcaggaa aggggagtcg gtgtggaata tctccacgcg
    26221 taatactttt gcccagctac ctaacagcgc atctgcaccg tcaaacaatg tgccattgcc
    26281 gataatttcg cgtagtgaac ccagcacgaa catggcgcag gttgcgccca taccaattga
    26341 aaagccgtcc agtgccgaaa gcgccggacc ttttttggcg gcgaaggctt cagcgcggcc
    26401 cacaacgata cagttagtga caatcagcgg aataaaaatc cctaatgatt gatacaggcc
    26461 aaaggcgtag gcgttgatca gcatctgtac agcgctgacc accgaggcga tgatcatcac
    26521 gtaaatggga atgcggatct cggctggcgt ccagtgacgc agcgtcgaaa tggtcaagtt
    26581 ggtcagcgtc agtaccagcg tagtcgcaag tcctaaaccc agagcgttag tggcagtgga
    26641 cgtgaccgcc aatagcggac agaggccgag caactggacc agcgcagagt tgtttttcca
    26701 caacccctga acaataacgt ctttaatttc gctcatgctt tattctccac aggcaggaag
    26761 ttgagaaagt tgtgccggta acgtctgagc gtacaatccg gcgcgtttta ccgcatttac
    26821 caccgcgcgg ggagtaatcg tcgcgccggt gaactggtcg aaatcaccac catctttctt
    26881 caccgcccag tttgcatcat ccgcaccact gattttttta cccgcaaaat gggtgatcca
    26941 gtcagaaagg cgcagttcga ttttatcgcc aagccctggc gtttcgtggt gctctgtcac
    27001 gcgcgtgcca agtaccgtgc cgttaaaatc ggctcccacc agcagctgaa tcgcaccgga
    27061 atagccatct ggcgcggttg tttccagaac ggcggctacc ggtttgtcat cctgtttggc
    27121 gatgtaaacc cgatgctcac ctttacctaa ctctggcgca gtgaccagat agcaactctg
    27181 tgccagcgca ttgttatagc gttcggctgg cagcacctga tcaaataacg ccttttgttg
    27241 cagactggcc tgttcagcaa ttgtcgtttt ggtcatctgg ttgatggccg cagttaaccc
    27301 tgtagaaccc gctgcaaata gtgccagtgt aatgccgtgt tttcggatag ttttcagcat
    27361 ggtttaccct ttgcgatggc cgtagacgcg cggacgcgtg tagtaatcga tcagaggaac
    27421 cgtgatgttc gccagcagga cggcaaaagc cacgccgtca ggatagccgc cgaaactgcg
    27481 gatcaaccag actaataaac ccgccagcgc gccgaacatc agacgaccac gattggtcgt
    27541 agaagcagta accgggtcag tcaaaataaa gaatgcgccg agcatagtcg caccagacag
    27601 cagatgaatt tgcggtgatg ccagtgtttc tggtgagaac aaccagccca acgttgcgca
    27661 taacgccagc gttactaaga agctgagggg aatatgccag cgaatcgctt tctgccatag
    27721 caaccacacg ccgccagcca gccaggcgag atttacccat tgccaaccaa cgcccgccag
    27781 cataccgctg tagatcggat attgcataat ctgttcaacc gaatgaccgg cacggacaga
    27841 ggttttaaat gtatccaacg gtgtcgcctg actaatgcca tcaataccta agcgtagcgt
    27901 gttcatatca ccaccactgg tggtatgtcc gctgaaaata acctgtatgg cgtcgataaa
    27961 accagggatg ttgaccgcaa tttcatgtgg cggtaaccag ctggtcatct gcaccgggaa
    28021 ggagatcagt aagaccacat aaccaatcat tgccggatta aacgggtttt gccccagacc
    28081 gccatacaac tgtttagcaa tgatcaccgc aaacaccgta cccagcacga ccatccacca
    28141 tggcgcgagg gggggaatac ttaccgccag caataagcct gtcagcaatg ctgagttatc
    28201 tttcaacgtt gcggctaccg actgcttgcg tagtttgagt acgagagctt cggctaacag
    28261 agcactgacc gacgccaaca ggatctgaac gagagtaccc caaccaaaaa accacagttg
    28321 cgctgcgatt cctggcacgg ctgcgagcaa caccaacagc ataatgcgcg atgtttggcg
    28381 ctggttatgg gtataagggg agctagctat tctgaatacc atttagtcct cgtttacaac
    28441 cttttgctgg gcggcttttt tggcctgaac gcgggcaata gccgcagcaa ctgccgcttt
    28501 gcgcggatca atctgttctt ctggctccgc attagcctgt tgctgttcca gcttgcgtgc
    28561 tttggcacgg gcaatagcag cttcgacggc ggctttgcgc ggatcaacct gttcttctgg
    28621 ttctgcgtta gcctgttgct gttccagctt gcgcgctttg gcacgggcaa tagccgcttc
    28681 gacggc
//
LOCUS       DAZFLD010000041        28253 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.041, whole genome shotgun sequence.
ACCESSION   DAZFLD010000041 DAZFLD010000000
VERSION     DAZFLD010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28253
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.041"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(113..1510)
                     /gene="ccp"
                     /locus_tag="ACHKBL_001496"
     CDS             complement(113..1510)
                     /gene="ccp"
                     /locus_tag="ACHKBL_001496"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HFO5818381.1"
                     /translation="MKMVSRITAIGLAGVAICYLGLSGYVWYHDNKRSKQADVQASAV
                     SENNKVLGFLREKGCDYCHTPSAELPAYYYIPGAKQLMDYDIKLGYKSFNLEAVRAAL
                     LANKPVSQSDLNKIEWVMQYETMPPTRYTALHWAGKVSDEERAEILAWIAKQRAEYYA
                     SNDTAPEHRNEPVQPIPQKLPTDAQKVALGFALYHDPRLSADSTISCAHCHALNAGGV
                     DGRKTSIGVGGAVGPINAPTVFNSVFNVEQFWDGRAATLQDQAGGPPLNPIEMASKSW
                     DEIIAKLEKDPQLKAQFLEVYPQGFSGENITDAIAEFEKTLITPDSPFDKWLRGDENA
                     LTAQQKKGYQLFKDNKCATCHGGIILGGRSFEPLGLKKDFNFGEITAADIGRMNVTKE
                     ERDKLRQKVPGLRNVALTAPYFHRGDVPTLDGAVKLMLRYQVGKELPQEDVDDIVAFL
                     HSLNGVYTPYMQDKQ"
     gene            1915..3564
                     /locus_tag="ACHKBL_001497"
     CDS             1915..3564
                     /locus_tag="ACHKBL_001497"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005993 - trehalose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HFO5818382.1"
                     /translation="MLNQKIQNPNPDELMIEVDLCYELDPYELKLDEMIEAEPEPEMI
                     EGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRD
                     FDLRKFVENHFWLPEVYSSEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
                     SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYY
                     LSRSQPPVFALMVELFEEDGVRGARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMP
                     DGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRD
                     TGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKACARRDAVNRY
                     LWDDENGIYRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGIL
                     ASEYETGEQWDKPNGWAPLQWMAIQGFKMYGDDLLGDEIARSWLKTVNQFYLEQHKLI
                     EKYHIADGVPREGGGGEYPLQDGFGWTNGVVRRLIGLYGEP"
     gene            complement(3615..4217)
                     /locus_tag="ACHKBL_001498"
     CDS             complement(3615..4217)
                     /locus_tag="ACHKBL_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFO5818383.1"
                     /translation="MQIVMFDRQSIFIHGMKISLQQRIPGVSIQGASQADELWQKLES
                     YPEALVMLDGDQDGEFCYWLLQKTVVQFPEVKVLITATDCNKRWLQEVIHFNVLAIVP
                     RDSTVETFALAVNSAAMGMMFLPGDWRTTPEKDIKDLKSLSARQREILTMLAAGESNK
                     EIGRALNISTGTVKAHLESLYRRLEVKNRTQAAMMLNISS"
     gene            4737..5636
                     /locus_tag="ACHKBL_001499"
     CDS             4737..5636
                     /locus_tag="ACHKBL_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO5818384.1"
                     /translation="MDKIHAMQLFIKVAELESFSRAADFFALPKGSVSRQIQALEHQL
                     GTQLLQRTTRRVKLTPEGMTYYQRAKDVLSNLSELDGLFQQDATSISGKLRIDIPPGI
                     AKSLLLPRLSEFLYLHPGIELELSSHDRPVDILHDGFDCVIRTGALPEDGVIARSLGK
                     LTMVNCASPHYLTRFGYPQSPDDLTSHAIVRYTPHLGVHPLGFEVASVNGVQWFKSGG
                     MLTVNSRENYLAAGLAGLGIIQIPRIAVREALRAGRLIEVLPGYRAEPLSLSLVYPQR
                     RELSRRVNLFMQWLAGVMKEHLD"
     gene            5685..6698
                     /gene="yhjD"
                     /locus_tag="ACHKBL_001500"
     CDS             5685..6698
                     /gene="yhjD"
                     /locus_tag="ACHKBL_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YhjD"
                     /protein_id="HFO5818385.1"
                     /translation="MTQENEIKRPTQDLEHEPIKQLDNSEKGGKVSQALETVTTTAEK
                     VQRQPVIAHLIRATERFNDRLGNQFGAAITYFSFLSMIPILMVSFAAGGFVLASHPML
                     LQDIFDKILQNISDPTLAATLKNTINTAVQQRTTVGLVGLAVALYSGINWMGNLREAI
                     RAQSRDVWERSPQDQEKFWVKYLRDFISLIGLLIALIVTLSITSVAGSAQQMIISALH
                     LNSIEWLKPTWRLIGLAISIFANYLLFFWIFWRLPRHRPRKKALIRGTFLAAIGFEVI
                     KIVMTYTLPSLMKSPSGAAFGSVLGLMAFFYFFARLTLFCAAWIATAEYKDDPRMPGK
                     TQP"
     gene            7091..8413
                     /locus_tag="ACHKBL_001501"
     CDS             7091..8413
                     /locus_tag="ACHKBL_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO5818386.1"
                     /translation="MQATATTLDHEQEYTPINSRNKVLVASLIGTAIEFFDFYIYATA
                     AVIVFPHIFFPQGDPTAATLQSLATFAIAFVARPIGSAVFGHFGDRVGRKATLVASLL
                     TMGISTVVIGLLPGYATIGIFAPLLLALARFGQGLGLGGEWGGAALLATENAPPRKRA
                     LYGSFPQLGAPIGFFFANGTFLLLSWLLTDEQFMSWGWRVPFIFSAVLVIIGLYVRVS
                     LHESPVFEKVAKAKKQVKIPLGTLLTKHVRVTVLGTFIMLATYTLFYIMTVYSMTFST
                     AAAPVGLGLPRNEVLWMLMMAVIGFGVMVPVAGLLADAFGRRKSMVIITTLIILFALF
                     AFNPLLGSGNPILVFAFLLLGLSLMGLTFGPMGALLPELFPTEVRYTGASFSYNVASI
                     LGASVAPYIAAWLQTNYGLGAVGLYLAAMAGLTLIALLLTHETRHQSL"
     gene            complement(8592..10652)
                     /locus_tag="ACHKBL_001502"
     CDS             complement(8592..10652)
                     /locus_tag="ACHKBL_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HFO5818387.1"
                     /translation="MSKAGKITAAISGAFLLLIVVAIILIATFDWNRLKPTINQKVSA
                     ELNRPFAIRGDLGVVWERQKQETGWRSWVPWPHVHAEDIILGNPPDIPEVTMVHLPRV
                     EATLAPLALLTKTVWLPWIKLEKPDARLIRLSEKNNNWTFNLASDDNKDTNAKPSAWS
                     FRLDNILFDQGRIAIDDKVSKADLEIFVDPLGKPLPFSEVTGSKGKADKEKVGDYVFG
                     LKAQGRYNGEPLTGTGKIGGMLALRGEGTPFPVQADFRSGNTRVAFDGVVNDPMKMGG
                     VDLRLKFSGDSLGDLYELTGVLLPDTPPFETDGRLVAKIDTEKSSVFDYRGFNGRIGD
                     SDIHGSLVYTTGKPRPKLEGDVESRQLRLADLGPLIGVDSGKGAEKSKRSEQKKGEKS
                     VQPAGKVLPYDRFETDKWDVMDADVRFKGRRIEHGSSLPISDLSTHIILKNADLRLQP
                     LKFGMAGGSIAANIHLEGDKKPMQGRADIQARRLKLKELMPDVELMQKTLGEMNGDAE
                     LRGSGNSVAALLGNSNGNLKLLMNDGLVSRNLMEIVGLNVGNYIVGAIFGDDEVRVNC
                     AAANLDIANGVARPQIFAFDTENALINVTGTASFASEQLDLTIDPESKGIRIITLRSP
                     LYVRGTFKNPQAGVKAGPLIARGAVAAALATLVTPAAALLALISPSEGEANQCRTILS
                     QMKK"
     gene            complement(10722..11489)
                     /gene="pdeH"
                     /locus_tag="ACHKBL_001503"
     CDS             complement(10722..11489)
                     /gene="pdeH"
                     /locus_tag="ACHKBL_001503"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase"
                     /protein_id="HFO5818388.1"
                     /translation="MIRQVIQRISNPEASIESLQERRFWLQCERAYTWQPIYQTCGRL
                     MAVELLTVVTHPLNPSQRLPPDRYFTEITVSHRMEVVKEQIDLLAQKADFFIEHDLLA
                     SVNIDGPTLIALRQQPKILRQIERLPWLRFELVEHIRLPKDSTFASMCEFGPLWLDDF
                     GTGMANFSALSEVRYDYIKIARELFVMLRQSPEGRTLFSQLLHLMNRYCRGVIVEGVE
                     TPEEWRDVQNSPAFAAQGWFLSRPAPIETLNTAVLAL"
     gene            11721..12650
                     /gene="kdgK"
                     /locus_tag="ACHKBL_001504"
     CDS             11721..12650
                     /gene="kdgK"
                     /locus_tag="ACHKBL_001504"
                     /EC_number="2.7.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygluconokinase"
                     /protein_id="HFO5818389.1"
                     /translation="MSKKIAVIGECMIELSEKGADVKRGFGGDTLNTSVYIARQVDPA
                     ALTVHYVTALGTDSFSQQMLDAWHGENVDTSLTQRMENRLPGLYYIETDSTGERTFYY
                     WRNEAAAKFWLESEQSAAICEELANFDYLYLSGISLAILSQTSREKLLSLLRECRANG
                     GKVIFDNNYRPRLWASKEETQQVYQQMLECTDIAFLTLDDEDALWGQQPVEDVIARTH
                     NAGVKEVVVKRGADSCLVSIAGEGLVDVPAVKLPKEKVIDTTAAGDSFSAGYLAVRLT
                     GGSAEDAAKRGHLTASTVIQYRGAIIPREAMPA"
     gene            complement(12746..14242)
                     /locus_tag="ACHKBL_001505"
     CDS             complement(12746..14242)
                     /locus_tag="ACHKBL_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFO5818390.1"
                     /translation="MQGTKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQW
                     QVLTTPQRPSDRVEIRLLVNTGSLAESTQQSGYSHAIPRIALTQSGGLEAAQARSLWQ
                     QGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINHAL
                     QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAM
                     TLLVVGNVDARSVVDQINKTFGELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLS
                     IMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSASNSKDIGLGFDCRVLYLRA
                     QCAINIESPNDKLNSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARA
                     DTDILMGQRMRSLQNQVVDIAPEQYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILL
                     QPKGEPEFNMKALQAAWDQIMAPSTAAAATSVATDDVHPEVTDIPPVQ"
     gene            complement(14463..15749)
                     /gene="dctA"
                     /locus_tag="ACHKBL_001506"
     CDS             complement(14463..15749)
                     /gene="dctA"
                     /locus_tag="ACHKBL_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DctC"
                     /protein_id="HFO5818391.1"
                     /translation="MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKL
                     IKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLYFEIVSTIALIIGLIIVNVVQPG
                     AGMNVDPATLDAKAVAVYADQAKDQGIVAFIMDVIPASVIGAFASGNILQVLLFAVLF
                     GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGAFGAMAFTIGKYGVGTLVQ
                     LGQLIICFYITCILFVVLVLGSIAKATGFSIFKFIRYIREELLIVLGTSSSESALPRM
                     LDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQATNSQMDIVHQITLLI
                     VLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNG
                     VATIVVAKWVKELDHKKLDDVLNNRAPDGKTHELSS"
     gene            complement(15932..17920)
                     /gene="hmsP"
                     /locus_tag="ACHKBL_001507"
     CDS             complement(15932..17920)
                     /gene="hmsP"
                     /locus_tag="ACHKBL_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator HmsP"
                     /protein_id="HFO5818392.1"
                     /translation="MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTAT
                     QLESIARSVREPLSSAILKGDIPEAEAILASIKPAGVVSRADVVLPNQFQALRKSFIP
                     ERPVPVMVTRLFELPVQISLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSTLV
                     TIYLLLSLILTVAISWCINRLILHPLRNIARELNAIPAQELVGHQLALPRLHQDDEIG
                     MLVRSYNLNQQLLQRHYEEQNENAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIIT
                     CETLRDTAGVLKEAQREILLLTLVEKLKSVLSPRMILAQISGYDFAVIANGVQEPWHA
                     ITLGQQVLTIMSERLPIERIQLRPHCSIGVAMFYGDLTAEQLYSRAISAAFTARHKGK
                     NQIQFFDPQQMEAAQQRLTEESDILNALENHQFAIWLQPQVEMTSGKLVSAEVLLRIQ
                     QPDGSWDLPDGLIDRIECCGLMVTVGHWVLEESCRLLAAWQERGIMLPLSVNLSALQL
                     MHPNMVADMLELLTRYRIQPGTLILEVTESRRIDDPHAAVAILRPLRNAGVRVALDDF
                     GMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPEDSSMIAAIIMLAQSLNLQMIAEGVE
                     TEAQRDWLAKAGVGIAQGFLFARPLPIEIFEESYLEEK"
     gene            complement(18002..21493)
                     /gene="bcsC"
                     /locus_tag="ACHKBL_001508"
     CDS             complement(18002..21493)
                     /gene="bcsC"
                     /locus_tag="ACHKBL_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase complex outer membrane
                     protein BcsC"
                     /protein_id="HFO5818393.1"
                     /translation="MRKFTLNIFTLSLGLAVMPMVEAAPTAQQQLLEQVRLGEATHRE
                     DLVQQSLYRLELIDPNNPDVVAARFRSLLRQGDIDGAQKQLDRLSQLAPSSNAYKSSR
                     TTMLLSTSDGRQALQQARLQATTGHAEEAVASYNKLFNGAPPEGDIAVEYWSTVAKIP
                     ARRGEAINQLKRINADTPGNTGLQNNLALLLFSSDRRDEGFAVLEQMAKSNAGREGAS
                     KIWYGQIKDMPVSDASVQALKKYLSIFSDGDSVAAAQSQLAEQQKQLADPAFRARAQG
                     LAAVDSGMAGKAIPELQQAVRANPKDSEALGALGQAYSQKGDRANAVANLEKALALDP
                     HNSNNDKWNSLLKVNRYWLAIQQGDAALKANNPDRAERLFQQARNVDNTDSYAVLGLG
                     DVAMARKDYPAAERYYQQTLRMDSGNTNAVRGLANIYRQQSPEKAEAYIASLSASQRR
                     SIDDIERSLQNDRLAQQAEALENQGKWAQAAALQRQRLALDPGSVWITYRLSQDLWQA
                     GQRSQAGQRSQADTLMRNLAQQKPNDPEQVYAYGLYLSGHDQDRAALAHINSLPRAQW
                     SSNIQELVNRLQSDQVLETANRLRESGKEAEAEAMLRQQPPSTRIDLTLADWAQQRRD
                     YTAARAAYQNVLTREPTNADAILGLTEVDIAAGDKAAARSQLAKLPATDNASLNTQRR
                     VALAQAQLGDTAAAQQTFNKLIPLAKSQPPSMESAMVLRDGAKFEAQAGDPTQALETY
                     KDAMVASGVTTTRPLDNDTFTRLTRNDEKDDWLKRGVRSDAADLYRQQDLNVTLEHDY
                     WGSSGTGGYSDLKAHTTMLQVDAPYSDGRMFFRSDFVNMNVGSFSTNADGKWDDNWGT
                     CTLQDCSGNRSQSDSGASVAVGWRNDTWSWDIGTTPMGFNVVDVVGGISYSDDIGPLG
                     YTVNAHRRPISSSLLAFGGQKDSPSNTGKKWGGVRADGVGLSLSYDKGEANGVWASLS
                     GDQLTGKNVEDNWRVRWMTGYYYKVINQNNRRVTIGLNNMIWHYDKDLSGYSLGQGGY
                     YSPQEYLSFAIPVMWRERTENWSWELGASGSWSHSRTKTMPRYPLMNLIPTDWQEEAA
                     RQSNDGGSSQGFGYTARALLERRVTSNWFVGTAIDIQQAKDYAPSHFLLYVRYSAAGW
                     QGDMDLPPQPLIPYADW"
     gene            complement(21475..22581)
                     /gene="bcsZ"
                     /locus_tag="ACHKBL_001509"
     CDS             complement(21475..22581)
                     /gene="bcsZ"
                     /locus_tag="ACHKBL_001509"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase complex periplasmic
                     endoglucanase BcsZ"
                     /protein_id="HFO5818394.1"
                     /translation="MNVLRSGLVTMLLLAAFSVQAACTWPAWEQFKKDYISQEGRVID
                     PSDARKITTSEGQSYGMFFALAANDRAAFDNLLDWTQNNLAQGSLKEHLPAWLWGKKE
                     NSKWEVLDSNSASDGDVWMAWALLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLG
                     SMLLPGKVGFAEDNSWRFNPSYLPPTLAQYFTRFGAPWTTLRETNQRLLLETAPKGFS
                     PDWVRYEKDKGWQLKAEKTLISSYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTE
                     KNGYPPEKVDVATGKAQGKGPVGFSAAMLPFLQNRDAQAVQRQRVADNFPGSDAYYNY
                     VLTLFGQGWDQHRFRFSTKGELLPDWGQECANSH"
     gene            complement(22588..24927)
                     /gene="bcsB"
                     /locus_tag="ACHKBL_001510"
     CDS             complement(22588..24927)
                     /gene="bcsB"
                     /locus_tag="ACHKBL_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HFO5818395.1"
                     /translation="MKRKIFWICAVALGMSAFPSFMTQATPATQPLINAEPAVAAQAE
                     QNPQVGQVMPGVQGADAPVVAQNGPSRDVKLTFAQIAPPPGSMVLRGINPNGSIEFGM
                     RSDEVVTKAMLNLEYTPSPSLLPVQSQLKVYLNDELMGVLPVTKEQLGKKTLAQMPIN
                     PLFITDFNRVRLEFVGHYQDVCENPASTTLWLDVGRSSGLDLTYQTLNVKNDLSHFPV
                     PFFDPRDNRTNTLPMVFAGAPDVGLQQASAIVASWFGSRSGWRGQNFPVLYNQLPDRN
                     AIVFATNDKRPDFLRDHPAVKAPVIEMINHPQNPYVKLLVVFGRDDKDLLQAAKGIAQ
                     GNILFRGESVVVNEVKPLLPRKPYDAPNWVRTDRPVTFGELKTYEEQLQSSGLEPAAI
                     NVSLNLPPDLYLMRSTGIDMDINYRYTMPPVKDSSRMDISLNNQFLQSFNLSSKQEAN
                     RLLLRIPVLQGLLDGKTDVSIPALKLGATNQLRFDFEYMNPMPGGSVDNCITFQPVQN
                     HVVIGDDSTIDFSKYYHFIPMPDLRAFANAGFPFSRMADLSQTITVMPKAPNEAQMET
                     LLNTVGFIGAQTGFPAINLTVTDDGSTIQGKDADIMIVGGIPDKLKDDKQIDLLVQAT
                     ESWVKTPMRQTPFPGIVPDESDRAAETQSTLTSSGAMAAVIGFQSPYNDQRSVIALLA
                     DSPRGYEMLNDAVNDSGKRATMFGSVAVIRESGINSLRVGDVYYVGHLPWFERLWYAL
                     ANHPILLAVLAAISVVLLAWVLWRLLRIISRRRLNPDNE"
     gene            complement(24938..27556)
                     /gene="bcsA"
                     /locus_tag="ACHKBL_001511"
     CDS             complement(24938..27556)
                     /gene="bcsA"
                     /locus_tag="ACHKBL_001511"
                     /EC_number="2.4.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417990.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016760 - cellulose synthase (UDP-forming)
                     activity [Evidence IEA];
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
                     /protein_id="HFO5818396.1"
                     /translation="MSILTRWLLIPPVNARLIGRYRDYRRHGASAFSATLGCFWMILA
                     WIFIPLEHPRWQRIRAEHKNLYPHINASRPRPLDPVRYLIQTCWLLIGASRKETPKPR
                     RRAFSGLQNIRGRYHQWMNELPERVSHKTQHLDEKKELGHLSAGARRLILGIIVTFSL
                     ILALICVTQPFNPLAQFIFLMLLWGVALIVRRMPGRFSALMLIVLSLTVSCRYIWWRY
                     TSTLNWDDPVSLVCGLILLFAETYAWIVLVLGYFQVVWPLNRQPVPLPKDMSLWPSVD
                     IFVPTYNEDLNVVKNTIYASLGIDWPKDKLNIWILDDGGREEFRQFAQNVGVKYIART
                     THEHAKAGNINNALKYAKGEFVSIFDCDHVPTRSFLQMTMGWFLKEKQLAMMQTPHHF
                     FSPDPFERNLGRFRKTPNEGTLFYGLVQDGNDMWDATFFCGSCAVIRRKPLDEIGGIA
                     VETVTEDAHTSLRLHRRGYTSAYMRIPQAAGLATESLSAHIGQRIRWARGMVQIFRLD
                     NPLTGKGLKFAQRLCYVNAMFHFLSGIPRLIFLTAPLAFLLFHAYIIYAPALMIALFV
                     LPHMIHASLTNSKIQGKYRHSFWSEIYETVLAWYIAPPTLVALINPHKGKFNVTAKGG
                     LVEEEYVDWVISRPYIFLVLLNLVGVAVGIWRYFYGPPTEMLTVVVSMVWVFYNLIIL
                     GGAVAVSVESKQVRRSHRVEMTMPAAIAREDGHLFSCTVQDFSDGGLGIKINGQAQIL
                     EGQKVNLLLKRGQQEYVFPAQVARVMGNEVGLKLMPLTTQQHIDFVQCTFARADTWAL
                     WQDSYPEDKPLESLLDILKLGFRGYRHLAEFAPSSVKGIFRVLTSLVSWVVSFIPRRP
                     ERSETAQPSDQALAQQ"
     gene            complement(27553..>28253)
                     /gene="bcsQ"
                     /locus_tag="ACHKBL_001512"
     CDS             complement(27553..>28253)
                     /gene="bcsQ"
                     /locus_tag="ACHKBL_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsQ"
                     /protein_id="HFO5818397.1"
                     /translation="TAALAWSLQMLGENVLVVDACPDNLLRLSFNVDFTHRQGWARAM
                     LDGQDWRDAGLRYTSQLDLLPFGQLSIEEQENPQHWQTRLSDICTGLQQLKASGRYQW
                     ILIDLPRDASQITHQLLSLCDHSLAIVNVDANCHIRLHQQALPDGAHILINDFRIGSQ
                     VQDDIYQLWLQSQRRLLPMLIHRDEAMAECLAAKQPVGEYRSDALAAEEILTLANWCL
                     LNYSGLKTPVGSAS"
BASE COUNT         6649 a         7796 c         7118 g         6690 t
ORIGIN      
        1 agcagaaaaa cgacctttta taatataaac atttaatatg ataatattaa aaacagactt
       61 tattatcgta ataaaaatcg cccaaacact cctgttcggg cgatcaaatt aattattgtt
      121 tatcctgcat atacggcgtg taaaccccgt tcagactgtg caggaaagct acgatatcat
      181 ccacatcctc ctgcggcagc tctttgccta cctgatagcg cagcatcagt ttcaccgccc
      241 cgtccagcgt cggcacgtca ccgcgatgga agtacggtgc cgttaaagca acgttacgta
      301 aaccgggtac tttctgacgc aatttatcac gctcttcttt agtaacattc atacgaccaa
      361 tatccgccgc cgtaatttca ccaaagttaa agtctttttt cagccccaac ggttcaaagg
      421 aacgtccgcc gagaataata ccaccatgac aagttgcaca tttattatct ttaaataatt
      481 gatagccttt tttctgttgc gccgtcagag cattctcatc tccacgcaac catttatcaa
      541 atggggaatc cggcgtaatt aatgttttct caaattcagc aatggcatca gtaatatttt
      601 cgccactgaa accttgtgga tagacttcga ggaactgcgc tttaagctgc ggatcttttt
      661 ccagcttagc aataatttcg tcccaggatt tcgacgccat ttcaatcggg ttcaacggcg
      721 gtccaccagc ctgatcctgc aatgttgccg cacgaccatc ccagaactgc tcaacgttaa
      781 atactgagtt aaataccgtc ggcgcgttaa tcggcccaac tgcgccacca acaccaatcg
      841 atgtttttct gccatcgacg ccccccgcat tcaacgcatg gcaatgagcg catgaaatgg
      901 tgctatcagc cgataaacgg ggatcgtgat acagcgcaaa acccaacgcc actttttgcg
      961 catcggtagg cagtttttgc gggatgggct gcaccggttc attgcgatgc tccggagcag
     1021 tatcattgct ggcgtaatat tccgcgcgct gttttgcaat ccaggccagt atttccgccc
     1081 gctcttcatc actcacctta cccgcccagt gtagcgcggt ataacgcgtt ggcggcatag
     1141 tttcatactg catcacccat tcaatcttat tcagatcgct ttgcgaaacg ggtttattag
     1201 ccagcagagc cgcacgcacg gcctcgaggt taaaagattt atatccaagc tttatgtcgt
     1261 aatccatcaa ctgtttcgcg ccgggaatat aataataggc gggtaattct gccgaaggtg
     1321 tatggcaata gtcgcatcct ttttcacgga gaaagcctaa aaccttatta ttttcactga
     1381 cagcagatgc ctgaacatcg gcctgtttac tgcgtttatt atcgtggtac cacacataac
     1441 cagataaccc taaatagcaa atcgcgacgc cagccaggcc gatcgcggta atacgtgaga
     1501 ccattttcat tattcttccc tcacggtggt gagttattgt gacccagaaa attttccaga
     1561 atgctgcaaa cgtctctgcg agacaaagcc atcataaaaa acagggagat atgctttttg
     1621 ataggaatca atcaattaca tgaatataaa ctatcaataa gatagccagt gcctattacc
     1681 cagatcagga atggcatatc atgattgcgg cgagagcagg attgaatgtt ctgaaaatga
     1741 aaaacaacac gaaatccgta tttttataaa ttccattgat attagtgcgt tatatttttt
     1801 ggcaggttaa taaacgtgat ccaccgcacg ctttgtcgcc caccaggcgg agcgaatgac
     1861 tacccttaaa gaaaagcccg ataattagcg acgaatttcg gaggtcggat ccttatgctc
     1921 aatcagaaaa ttcaaaaccc taatccagac gaactgatga tcgaggtcga tctctgctat
     1981 gaactggacc catatgaatt aaaactggat gagatgatcg aggcagaacc ggaacccgag
     2041 atgattgaag ggctgcccgc ctctgatgcg ctgacgcctg ccgatcgcta tctcgaactg
     2101 ttcgaacatg ttcagtcggc gaaaattttc cccgacagta aaacctttcc cgactgcgca
     2161 cctaaaatgg acccgctgga tatcttaatc cgctaccgta aagtgcgccg tcatcgtgat
     2221 tttgacttgc gcaagtttgt tgaaaaccac ttctggctgc cggaggtcta ctccagcgag
     2281 tatgtatcgg acccgcaaaa ttccctgaaa gagcatatcg accagctgtg gccggtgcta
     2341 acccgcgaac cgcaggatca cattccgtgg tcttctctgc tggcgctgcc gcagtcatat
     2401 attgtcccgg gcggccgttt tagcgaaacc tactattggg attcctattt caccatgctg
     2461 gggctggcgg aaagtggtcg ggaagatttg ctgaaatgca tggccgataa cttcgcctgg
     2521 atgatcgaaa actacggtca catccccaac ggcaaccgca cctattattt gagccgctcg
     2581 caaccaccgg tttttgcgct gatggtggag ttgtttgaag aagatggtgt acgcggtgcg
     2641 cgccgctatc tcgaccacct gaaaatggaa tatgccttct ggatggacgg tgcagaatcg
     2701 ttgatcccta atcaggccta tcgccatgtt gtgcggatgc cggacggatc gctgctcaac
     2761 cgttactggg acgatcgcga cacaccgcgt gacgaatcct ggcttgagga cgttgaaacc
     2821 gcgaaacatt ctggtcgccc gcccaacgag gtgtaccgcg atttacgcgc gggggcggcc
     2881 tccggttggg attactcttc ccgctggctg cgtgatactg gtcgtctggc gagcattcgt
     2941 accacccagt tcatccccat cgatctgaat gccttcctgt ttaaactgga gagcgccatc
     3001 gccaacatct cggcgctgaa aggcgagaaa gagacagaag cactgttccg ccagaaggcc
     3061 tgtgcccgtc gcgatgcggt aaaccgttac ctctgggatg atgaaaacgg tatctaccgc
     3121 gattacgact ggcgacgcga gcaactggcg ctgttttccg ctgccgccat tgtgccgctc
     3181 tatgtcggta tggcgaacca tgaacaggcc gatcgtctgg caaacgccgt gcgcagccga
     3241 ttactgacac ctggcgggat tctggcaagc gagtacgaaa ccggtgaaca gtgggataaa
     3301 cccaacggct gggcaccgtt acaatggatg gcgattcagg gatttaaaat gtatggcgat
     3361 gaccttctgg gtgatgaaat cgcgcgcagc tggctgaaga cggtgaatca gttctatctg
     3421 gaacagcaca aactgatcga gaaataccat attgccgatg gtgttccccg cgaaggcggc
     3481 ggtggcgagt atccgttgca ggatgggttt ggctggacta acggtgtggt gcgccgttta
     3541 attggtttgt acggtgaacc ataatatttt tacagccagc cgctaacttc ctgctggctg
     3601 taaaattatc ctcttcagga ggagatattt aacatcattg ccgcctgggt gcgatttttc
     3661 acttccagac ggcgatacag ggattccaga tgcgctttta ccgttccggt actgatattc
     3721 agcgccctgc cgatctcctt atttgattcg cccgccgcta acatggttaa aatctcccgc
     3781 tggcgggcgc ttaacgattt gagatcttta atgtcctttt ccggcgtcgt ccgccagtct
     3841 ccaggcagaa acatcatccc catcgccgca ctatttaccg ccaacgcaaa tgtctcgacg
     3901 gttgaatcac gaggcacaat ggccagcaca ttaaaatgga taacttcctg taaccaccgt
     3961 ttattgcaat ccgtcgccgt aattaacacc ttaacctcag gaaattgcac cacggttttt
     4021 tgcagcaacc agtaacaaaa ctcaccatcc tgatcgccat cgagcatcac taaggcttca
     4081 gggtaacttt ccagcttttg ccataactcg tctgcctgac tggccccctg aatactcact
     4141 cctggaatac gttgctgtaa actgattttc attccatgaa taaatattga ctgcctgtca
     4201 aacatgacta tttgcataac tgaatctcca cctgaatacg ttaaaaagac ttaagtagtg
     4261 gaagggtatt acccgcgaga aaaaataaga attcgccatt tggcggtggc cattctacag
     4321 agatgacgcg tagaaaatag ttaccgatat aaatagttac agctaaacgc ctgaaattac
     4381 atgtcgaggg cactatttaa aacaattttg aggatttcct tatattagtg gttagtacgc
     4441 atgcaattaa aaatgaaatt ccgcgaccac aagccaaaat aacaaatggc aatgagacaa
     4501 aaataagcac aaatagccaa tacgtcctct attcacttta aagggaatcg ctgaaaaata
     4561 cgctctgttt aaggggattc acctttctaa gaaagctatt cctccctttt cctactgaga
     4621 aatcgccaca ttcgtcatga caacattgtg aaacccggct ttagatgtta gaaaaaacaa
     4681 taaacaatgc gatatgcgcg ttactgtttc gctgtgaaca acaatcggtc aaagaaatgg
     4741 ataaaattca cgcaatgcag ttgttcatca aagtcgcgga gctggaaagt ttttcccgcg
     4801 cggcggattt ctttgctttg ccaaagggaa gtgtttcgcg ccagatacag gcactggaac
     4861 atcaacttgg cacccagctt ctccagcgca ctacgcgacg ggtcaaactc acgccagaag
     4921 gcatgaccta ttatcaacgg gcaaaagatg tgttgagtaa tctcagcgaa ctggacggtc
     4981 tgtttcaaca ggatgccacc agtatcagcg gtaaattacg catcgacatc ccgccaggaa
     5041 tcgcgaaaag cctgttactg ccgcgcttgt cggaatttct ctatctgcat ccgggaattg
     5101 agctggaact gagtagccat gaccgtccgg tagatattct tcatgatggt tttgattgcg
     5161 tgatacgcac tggcgcatta ccggaagatg gcgttatcgc ccgttctctc ggcaaactga
     5221 ccatggtcaa ctgtgccagt ccacactatc tgacgcgctt tggttatccg caaagccccg
     5281 atgatctgac ttcacacgca atagtgcgtt atacaccgca cctgggtgta catccgttag
     5341 gttttgaggt tgccagcgtt aatggcgtcc agtggtttaa gtctggcggc atgttgacgg
     5401 taaacagtcg cgaaaactat ctcgccgccg gtcttgccgg tctggggatt attcagatcc
     5461 cgcgcattgc cgtgcgcgaa gccctgcgtg ccgggcggct tattgaagtg ttgcctggct
     5521 accgtgccga gccgctctcc ctttcactgg tttatccgca gcgtcgggag ctttcccggc
     5581 gtgtaaacct gtttatgcag tggctggctg gcgtaatgaa agagcacctg gactgaccga
     5641 ctatactttt taagaatgac cacgacagaa ggacaaaaga gcggatgacg caggaaaacg
     5701 agatcaaacg tcccacccag gatctggagc acgagccgat taagcagttg gataatagcg
     5761 agaaaggcgg caaagttagc caggcgctgg aaaccgtcac cactactgcc gagaaagtcc
     5821 aacgccaacc ggttattgcg cacctgattc gcgcaacaga gcgctttaac gatcggctgg
     5881 gtaaccagtt tggtgcggct atcacctatt tctcattttt gtcgatgatc ccgattttga
     5941 tggtatcgtt tgccgcaggg ggctttgtgc tggcctccca tccgatgttg ctacaggata
     6001 tcttcgacaa aattctgcaa aacatcagcg atccgacgct agccgccacg ttgaaaaaca
     6061 ccatcaacac cgccgttcag cagcgtacta ccgtagggct tgtcggcctg gcggtggcgc
     6121 tttattccgg catcaactgg atgggtaacc tgcgtgaagc gattcgcgcc cagtcacgcg
     6181 atgtctggga acgctcgccg caagatcagg agaagttctg ggtaaaatat ctgcgtgatt
     6241 ttatttcgct gattggtttg ttgattgcgc tgattgtgac gctttcgatc acctcggttg
     6301 ccggttcggc gcagcaaatg attattagcg ccctgcacct gaacagcatt gagtggctga
     6361 aaccgacgtg gcggttgatt ggcctggcga tttccatctt cgccaactat ctgcttttct
     6421 tttggatctt ctggcgcttg ccgcgccacc gtccgcgcaa aaaagccctg attcgcggaa
     6481 catttctggc tgcgattggt tttgaagtga ttaaaatcgt gatgacctac accctgccat
     6541 cgctgatgaa atccccctct ggcgcagcat ttggttccgt gctgggactg atggcatttt
     6601 tctacttctt cgcccgtttg acgctgtttt gcgcggcgtg gattgccacc gccgaatata
     6661 aagacgaccc gcgaatgccg gggaaaacgc agccttaaaa taggccggat gcggcgtacg
     6721 aatttattcg gcctggctcc ccgtaggccg gataagatgc gccagcatcg catccggcta
     6781 taatgcgcac ataacctctt gaaactcttt cccagagcct ctttagccat ctattcagga
     6841 gcaaacaatt tcataccaac tcataacccc agcatataaa tccagttggt aacttttatt
     6901 taacctgaaa ccagttttat ccactattta taaaattatg tgaagcattt catagaagaa
     6961 aaatcactgg cctaaacatt atcccctttt tgcctggttt ttgaccattt ccgcgatttg
     7021 ttacacattg aaatatcact tttgctgtgc gtaatatggc tattcgttag ccaaaaaata
     7081 agaaaagatt atgcaagcaa cagccacaac actcgaccac gagcaagaat acacgccgat
     7141 caactcgcgt aataaagtcc ttgtcgcctc tctcattggc acggccattg agttcttcga
     7201 cttttacatt tacgccactg cggccgttat tgtgtttccg catatcttct tcccgcaggg
     7261 cgatcctacg gcagcaacgc tacagtcgct cgccaccttt gccatcgcct tcgtcgcgcg
     7321 ccccattggc tctgccgttt ttggtcattt tggcgatcgc gttgggcgta aagcgacgct
     7381 ggtcgcctcg ttgctaacga tggggatttc gaccgtggtg attggcctgc tgccaggcta
     7441 tgccacgatt ggtattttcg ccccgctgct gctggcgctg gctcgatttg gtcagggtct
     7501 gggcttaggt ggtgaatggg gcggcgcggc tctgctggcg actgaaaacg ccccaccgcg
     7561 caaacgtgca ttgtacggct ccttcccgca gctgggcgca ccgattggct tcttctttgc
     7621 caacggcact ttcttgctgc tttcctggct actgaccgac gagcagttta tgagctgggg
     7681 ctggcgtgtg ccatttatct tctcggcggt gctggtcatt atcggcctgt atgttcgcgt
     7741 gtcgctgcat gagtcgccgg tgtttgagaa agtcgctaaa gcgaaaaaac aggtgaagat
     7801 cccgctgggt acgctgctga ccaaacatgt tcgcgtaacc gtactgggta cgttcattat
     7861 gctggcaacc tatacgctgt tttacatcat gacggtctac tctatgacct ttagtaccgc
     7921 cgccgcgccc gttgggcttg gcctgccgcg taacgaagtg ttgtggatgc tgatgatggc
     7981 agttattggt tttggcgtga tggtgccagt cgccggatta ctggctgatg cctttggtcg
     8041 ccgtaaaagc atggtaatca tcaccacgct gatcatcctg ttcgcgctgt tcgcctttaa
     8101 cccgttgctc ggttctggca acccgattct ggtttttgcc ttcctgctgc tggggttaag
     8161 tctgatgggg ctgaccttcg gtccgatggg cgcgctgtta ccagagctgt tcccgacaga
     8221 agtgcgttac accggagcat cgttctctta caacgtagcg tcgattctcg gggcttccgt
     8281 tgcgccgtat atcgcagcct ggttgcagac taactacggg ttaggtgcgg tggggttata
     8341 tctggcggcg atggctggct tgacgttaat cgccctgctg ctgacccatg agacgcgaca
     8401 tcagtcgttg taataagagt gaaaagcctg atgcgctacg cttatcaggc cagaatttcc
     8461 atacaatata ttgaattagc aaggatttgt aagccggagg cgttcacgcc gcatccggca
     8521 tgaacaaagc acactttgcc aacaatctaa atctgtttaa ttgccggatg tcagacatcc
     8581 ggcaattatc atcacttctt catctgcgac aaaattgtcc tgcactgatt agcctcccct
     8641 tcggaaggtg agatcagcgc cagtaacgcc gccgccggtg ttaccagcgt tgccagtgcc
     8701 gccgcaacgg ctccgcgagc aatcaacggc ccggctttca ccccagcctg cggattttta
     8761 aacgtcccac gtacatacag cggcgaacgc aatgtgataa tccgaatccc tttactctcc
     8821 ggatcaatag tcaaatccag ctgttccgaa gcaaaacttg ccgtaccggt aacgttaatc
     8881 aacgcgttct cagtatcgaa agcaaaaatc tgcgggcgcg ccacgccgtt ggcaatatcc
     8941 agattcgccg ccgcacagtt cacccgcacc tcatcatcac caaatatcgc accgacaatg
     9001 tagttaccga cattcagccc gacaatctcc atcaggttgc ggctcaccag cccgtcattc
     9061 atcagcagtt tcaggttgcc gttactgttg cctaaaagcg ccgccaccga gttaccgcta
     9121 ccgcgtagtt ccgcgtcacc gttcatttcc cccagcgtct tctgcatcag ttctacatcg
     9181 ggcatcagtt ctttcagttt cagtcgacga gcctgaatat ctgcccgccc ttgcatcggt
     9241 tttttatcac cttccagatg aatattcgcc gcaatgctgc cgcctgccat gccaaatttc
     9301 agcggttgca ggcgcaggtc agcatttttg aggatgatat gagtagaaag atcgctaatc
     9361 ggcaggctac tgccatgctc aatgcgccgc cctttgaagc gaacatcggc atccatgacg
     9421 tcccatttat cggtttcgaa gcggtcataa ggcagcactt tgcccgcagg ctgaacgctt
     9481 ttttcaccct tcttctgttc agaccgtttc gacttttcag ctcctttccc ggaatcaacg
     9541 ccaattaacg gccctaaatc tgccagccgc aattgccgcg actcaacatc accttccagt
     9601 tttggtcgtg gcttgccggt ggtgtagacc agagaaccgt ggatatcgct atcgccaatt
     9661 cgcccattaa aaccgcgata atcaaagacc gacgattttt cagtgtcgat tttcgctacc
     9721 agccgaccat ccgtttcaaa cggcggggta tcgggcagca gaacgcccgt cagttcatag
     9781 agatcaccca gcgaatcgcc agaaaattta agccgtaaat cgacaccacc catcttcatt
     9841 gggtcattca caacgccatc aaaagcaaca cgggtgttac cagagcgaaa atcagcctgt
     9901 acaggaaatg gcgtcccttc gccacgcagc gccagcatac cgcctatttt tcccgtacca
     9961 gtgagcggct ctccgttata acgtccctgc gccttcaggc caaaaacgta atcgcccacc
    10021 ttttctttat ccgctttacc tttcgatcca gtaacttcgc tgaacggcag cggcttgcct
    10081 aacggatcga caaaaatttc tagatccgct ttgcttactt tgtcatcaat ggcgatccgc
    10141 ccttgatcga acagaatatt atccagccga aacgaccatg ccgacggctt tgcattcgtg
    10201 tctttgttgt catcgctggc gagattaaat gtccagttgt tgttcttttc tgagaggcga
    10261 atcaggcgtg cgtcgggctt ttcgagcttg atccacggca accagaccgt tttggtcagc
    10321 agcgccagcg gggccagcgt cgcttctact cgcggcaaat gcaccattgt gacttcggga
    10381 atatccggtg gattgccaag aatgatgtct tccgcgtgta catggggcca cggcacccag
    10441 ctgcgccagc cagtttcttg tttttgccgc tcccacacca cgcccagatc gccacggata
    10501 gcgaacggac gattcaactc cgcagagact ttctggttga tggtcggttt gagtcgattc
    10561 cagtcaaaag ttgcaatcaa aatgatcgcc acgacaatca acaacaagaa agcccctgaa
    10621 atcgcagcgg ttattttgcc tgccttgctc atcgttcgct tcctcatatt cttcctgtgc
    10681 cagtcctaaa gatagtccag ccaggcggaa aatgaggcag cttatagcgc cagaaccgcc
    10741 gtattcagcg tttctatcgg tgccgggcgt gaaagaaacc agccttgtgc ggcgaatgcg
    10801 ggcgagttct gaacatcacg ccactcttcc ggcgtttcta cgccctcgac aatcaccccg
    10861 cgacaatagc gattcatgag atgtaaaagc tgagaaaaga gtgtgcggcc ttccggcgac
    10921 tgacgcagca tcacgaacag ctctcgcgcg attttgatgt agtcataacg cacttcactt
    10981 agcgcagaga aatttgccat cccggtgcca aaatcatcca gccacagcgg gccaaattca
    11041 cacatcgagg caaaggttga atctttcggc agacggatat gctccaccag ttcgaaacgc
    11101 agccagggaa gacgctcaat ttggcgcagg atttttggtt gctgacgcag ggcgatgagc
    11161 gtagggccat caatattgac cgatgccagc aggtcgtgct ctataaagaa gtcggctttt
    11221 tgcgccagca aatcaatctg ctctttcaca acctccatcc gatggctaac ggtgatttca
    11281 gtaaaatagc gatccggcgg caggcgttgc gaagggttca agggatgcgt gaccaccgtt
    11341 aatagctcca cggccattaa ccgtccgcat gtttgataga tcggctgcca ggtgtaagca
    11401 cgctcacact gcaaccaaaa acgccgttcc tgcaagctct cgatgcttgc ttcagggttg
    11461 cttattcgct ggataacctg ccttatcatc aaagatgtcc tgtgtttaaa agtgatgctg
    11521 cccggactcg acaaagatta tcggcgcgca agggcagaac tttaccgcct ggtcggggta
    11581 aatttcccca ctccacaacg gaatctttca ggaatacgtt ctggctcaaa aaaataatgg
    11641 aacactgttt taatatggtt gaccagcaaa ccaccacagc gcaaactaac gctaattttt
    11701 tacagatcag gttcacgact atgtccaaaa agattgccgt gattggcgaa tgcatgattg
    11761 agctttccga gaaaggcgcg gacgttaagc gcggtttcgg cggcgatacc ctgaacactt
    11821 ccgtctatat cgcccgtcag gtcgatcctg cggcattaac cgttcattac gtaacggcgc
    11881 tgggaacgga cagttttagc cagcagatgc tggacgcctg gcacggcgag aacgttgata
    11941 cttccctgac ccaacggatg gaaaaccgtc tgccgggcct ttactacatt gaaaccgaca
    12001 gcaccggcga acgtacgttc tactactggc gtaacgaagc cgccgccaaa ttctggctgg
    12061 agagtgagca atccgcggcg atttgcgaag agctggcgaa tttcgattat ctctacctga
    12121 gcgggattag cctggcgatc ttaagccaga ccagccgcga aaagctgctt tccctgctgc
    12181 gcgaatgccg cgccaacggc ggaaaagtga ttttcgacaa taactatcgt ccgcgcctgt
    12241 gggccagcaa agaagagaca cagcaggtgt accaacaaat gctggaatgc acggatatcg
    12301 ccttcctgac gctggacgat gaagacgcgc tgtggggtca acagccggtg gaagacgtca
    12361 ttgcgcgcac ccataacgcg ggcgtgaaag aagtggtggt gaaacgcggg gcggattctt
    12421 gcctggtgtc cattgctggc gaagggttag tggatgttcc ggcggtaaaa ctgccgaaag
    12481 aaaaagtgat cgataccacc gcagctggcg actctttcag tgccggttat ctggcggtac
    12541 gtttgacggg cggcagtgcg gaagacgcag cgaaacgcgg gcacctgacc gcaagtaccg
    12601 ttattcagta tcgcggcgcg attatcccgc gtgaagcgat gccagcgtaa aagcagcata
    12661 tgcaaaaggc tggatagagt gttacccccc tatccagctc atccataaat acctgatggt
    12721 tgtgctaact catcaccatc aggcattact gtacaggtgg aatatccgtc acttcaggat
    12781 gtacgtcatc cgtggcgaca gatgtggcgg cagccgcagt cgatggggcc atgatttgat
    12841 cccaggccgc ctgcaacgct ttcatgttaa attccggctc gcctttcggc tgcagcagta
    12901 ttaacgccat atcattcgac aactgctgac gcagatcctg atttaacatc tccaccgtca
    12961 ggctgttaag gaaatcctga cgcagtttct gatattgctc cggcgcgata tcgacgacct
    13021 gattttgcaa cgaacgcatc cgctgcccca tcagaatatc ggtatcagct cgcgcatagg
    13081 cggcaaacag tttctgcagc tccagtttct tttgcgccac taacgcattg aactcttctt
    13141 ccggcagacc tttatcgcga accttcgcca gttcacgcgc caccagatta aggttgctgt
    13201 tcagcttgtc gtttggtgat tcgatgttga tggcacactg cgcacgcaga tacagcacac
    13261 gacagtcaaa tccaagaccg atgtctttac tgttactggc gcttaacgct tgctgaacat
    13321 gccagaacag cgcctcacgg gccaggtccg cacgccagta gcgcagcagt gcggctgatt
    13381 cacgaatcgg ctgccacggc gtatcccaca tgattgataa ccggtcctga cgcaccgcgt
    13441 cagtcataat actcaccgct tccgcacgca gcggagaaag cgtcggcacc ggagccggtg
    13501 tttcacgttt gcctttcagt tcgccaaacg ttttattgat ttggtcgacg accgagcgcg
    13561 catccacgtt tcccaccacc agcagcgtca ttgcatccgg ggtataccat ttctgataga
    13621 aatctttaat cttttccgct tcaacgggtt gtttcagcgg atcggcagga tcgtgaccta
    13681 acaaggttga tcctttcaga cgatagcgcc accagccctc tttagtatcg gcaggccagg
    13741 ttgccaccat gtcctgactt tgcagcgcgt ggttgatggt ttctggtgtg atggtcagtt
    13801 tgccagtggc atttgccaga taagagagcg cttctttcag caagtcgtta cggttattgg
    13861 gcaaactcag attaaacagc gtggtgtcat aagagacaat tactggcggc atcgggcgtt
    13921 tagggtcgat cccctgctgc cacaatgaac gcgcctgtgc tgcttcaagg ccaccgcttt
    13981 gcgttaacgc aatacgaggg atggcgtggc tgtaaccgct ctgttgtgta ctttcggcga
    14041 gcgaaccggt attaaccagc aggcgaattt caacacgatc gctaggacgc tggggcgtgg
    14101 tcagcacttg ccactgtaaa ccgttggaaa gcgtcccctg ttgccatgct ggatcaggct
    14161 ggagcgcatc tgcctgcaca tagccagcag tggccatcat cagcaaaccg cccgctaaaa
    14221 gtcgaatttt tgtgccctgc atgtgaaccc ctgatcaact atcctggtaa taaaaaaact
    14281 gcccgttgtc ggtcagtcta aatgacgtta aaaacacttc gtgttagacc gcaagaacat
    14341 gaaaatgtca cggaagaagt gaaataaacc tgaactcacc caggtggtaa gttcaggcac
    14401 aggggtcaat tatgcgcaaa cacccgcact cggggaaggg agtgcgggca taagtgatga
    14461 gattaagagg ataattcgtg cgttttgcca tccggcgcac gattattcag cacatcgtcc
    14521 agttttttgt ggtccagttc tttcacccac ttagcaacga caatggtcgc tacgccgtta
    14581 ccgaccaggt tagtcagcgc acgagcttct gacataaagc ggtcgatacc aaggatcagc
    14641 gccagacctg ctaccggcaa atggcccacc gcagagagcg tcgccgccag cacgataaag
    14701 ccactgcccg ttaccccggc agcccctttg gaagagagca acaacacaat taatagcgta
    14761 atttggtgga cgatatccat ctgactgtta gtcgcctggg cgataaacac cgccgccatt
    14821 gtcaggtata tcgatgtgcc atcaaggtta aacgagtagc ctgtcgggat gaccagcccc
    14881 accaccgatt tccggcagcc gagtttctcc atcttgtcga gcatacgcgg cagcgccgac
    14941 tcggaagaag aagtgcccag tacaatcagc agctcttcac ggatgtagcg gataaatttg
    15001 aagatactga aaccagtcgc tttagcgatt gaacccaata ccagcaccac aaacaggata
    15061 caggtgatgt agaaacagat aatcagctgc cccagttgca ccagtgtgcc gacgccgtat
    15121 ttaccgatgg taaacgccat tgccccgaac gcaccaatag gtgccagacg catgatcata
    15181 ttgatgatgc cgaagatgac ctgcgagaaa ctttcgatga cgttaaaaat cagttggcct
    15241 ttgctgccca gacggtggag cgcaaaacca aacagtacgg caaacagcag cacctgcaga
    15301 atgttaccgc tggcaaatgc gccaatgacg ctcgccggga tgacatccat aatgaaggcg
    15361 acaatgccct ggtctttcgc ctgatcggcg taaaccgcta ccgctttcgc atcaagcgtt
    15421 gccggatcga cgttcattcc ggcaccaggc tgcacgacgt taacgatgat aagaccaata
    15481 atcagcgcga tggtactgac aatttcaaag taaagcagtg cgactgcgcc ggtacgacca
    15541 accgccttca tgctttccat gcccgcaatg cccgttacga cggtacaaaa gatgacagga
    15601 gcgatgatca tcttaatgag cttaacgaac ccgtcgccaa gcggtttcat ttgctcgcct
    15661 atttcagggt agaaatggcc aaggagaata ccaatggcta tcgctgtcag gacctgaaag
    15721 taaaggcttt taaacagaga ggttttcata gggtgtcctt tagtaaaacc acaggtcttg
    15781 taaggttatg gggtacctgc ggccctaaaa taacacccag acaacatcac agaaatgtac
    15841 ctggatcata attgaaacaa aaaggttaaa aagtttgagc tggctcgcac aaaccagcac
    15901 tttttaaagt tttgtaatca gtttggggta gctacttttc ttccaggtaa ctctcttcga
    15961 agatttcaat agggagtggg cgagcaaaaa ggaagccctg ggcaatacca acgcccgctt
    16021 ttgccagcca gtcgcgttgt gcttcagtct ccacgccttc ggcaatcatt tgtaagttca
    16081 ggctttgcgc caacatgata attgcagcaa tcatgctgct atcttccggc aagccttcaa
    16141 caaacatttt gtcgattttc agtacgtcga ttggcaacga tttcatatgc tgcagctgac
    16201 gcagccctgc gtagcccatg ccgaaatcat ccagcgccac ccgaactccg gcattgcgca
    16261 gcggacggag gattgccacc gcagcatgag ggtcgtcaat acgtcggctt tctgtcactt
    16321 ccagaatcag tgttcccggc tgaatgcgat agcgggttaa cagttccagc atatccgcta
    16381 ccatattcgg gtgcatcagt tgtagcgctg agaggtttac cgacaagggc agcataatgc
    16441 cgcgctcttg ccaggcagca agcagtcgac aagactcttc cagcacccag tgaccaacgg
    16501 taaccatcag cccacagcac tcaatgcgat cgattaagcc atccggcaga tcccaactgc
    16561 catccggttg ctggatacgc agtaacactt ccgcactgac cagtttaccg ctggtcatct
    16621 cgacctgtgg ctgtaaccaa atcgcaaatt gatgattttc cagtgcatta aggatatcgc
    16681 tctcttccgt caaccgctgc tgggcggctt ccatctgctg cggatcaaag aactgaatct
    16741 gatttttgcc tttatggcga gcggtaaatg ccgcagaaat agcgcgactg taaagctgtt
    16801 cggcggtgag atcgccgtag aacatcgcca cgccaatgct acagtgcgga cggagttgaa
    16861 tacgttcaat cggcaggcgc tcgctcatga tagtgagcac ttgctgacct aatgtgatag
    16921 cgtgccacgg ttcctgtaca ccgttggcaa tgacagcaaa gtcataaccg ctaatctgcg
    16981 cgaggatcat gcgtggcgac agtaccgatt tgagtttttc caccagcgtc agcagcagaa
    17041 tttctcgttg cgcctctttc agcacgcccg cagtatcacg cagggtttca caggtgatga
    17101 tcatcagcgc ggtggtttgt ttacgcgcga caacctgctc cagcatctcc atcagcaagg
    17161 ctttgttcgg caaatccgac accgggaagc gcatcgcgtt ttcgttctgt tcttcataat
    17221 ggcgctgcag caattgctgg ttgaggttgt aactgcgcac cagcataccg atttcatcgt
    17281 cctgatgcag acgtggtaat gccagttggt gaccaacaag ctcctgggct gggatggcgt
    17341 taagttcgcg agcaatattg cgtaacggat gtaaaatcag tcgattaata caccagctga
    17401 tggcgacggt caggataagc gacaaaagta agtaaatggt cactaacgtt gagagggtgc
    17461 tcatcacgaa cttatacata cggaaggaat ccgcctgtag caccagatag gcaattggct
    17521 gcggatttgc cggacgttcg agcgagtaga cgcccagcga gatttgaacc ggtagctcaa
    17581 acaggcgagt aaccattacc ggcaccggac gctctggaat aaaactttta cgcagcgcct
    17641 ggaactggtt gggcagcacc acatcggcac ggctgaccac gcctgccggt ttaatgctgg
    17701 caagaatagc ttccgcttcg ggaatatcgc ctttcaaaat agctgaagat aagggttcgc
    17761 ggacagagcg agcaatgctt tccagttgcg tagccgtgtt atagcgattc tgctggacca
    17821 gatggaacag caaaacggtg caaaaaataa aaacgaacac caggacaacg gctgccacca
    17881 ttgccatctg cttgattgtt aacgagcgac ttacgcgcaa attgtctccc cccggaaatc
    17941 ccagattgcc gcccgagtat acccgatcgc agcggcatta agagaggcgc tatctgaaaa
    18001 cttaccagtc ggcgtaaggt atcagcggct gcggcggtaa atccatgtca ccctgccatc
    18061 cggcggcgga ataacgtacg tagagcagga aatggctggg tgcgtaatct ttcgcctgct
    18121 ggatatcaat tgccgtgccg acaaaccagt tggaagtaac acgtcgttca agtaatgccc
    18181 gcgccgtata gccgaagccc tgactgctgc cgccatcgtt ggattgccgc gcagcttctt
    18241 cctgccagtc ggtcgggatc agattcatca gcggataacg cggcatggtt ttggtgcgtg
    18301 aatgcgacca cgagccagac gcccccagct cccacgacca gttttccgtg cgctcccgcc
    18361 acatcaccgg tatggcaaac gacaggtatt cctgcgggct gtagtaaccg ccctgaccga
    18421 gtgagtagcc actcaggtct ttgtcgtaat gccagatcat gttgttcagg ccgattgtga
    18481 cgcggcgatt gttctggtta atgaccttat agtaatagcc cgtcatccag cgcacacgcc
    18541 agttatcttc gacattttta ccggttaact ggtcgccact aagcgatgcc cagacgccgt
    18601 ttgcttcacc tttatcgtag ctcaggctta gccccacgcc gtcggcacgc acgccgcccc
    18661 attttttccc ggtattgctc ggggagtctt tttgcccacc aaaggccagc aaagaactgg
    18721 agatgggccg acggtgggcg ttaacggtgt aacccagcgg cccgatatca tcgctgtaac
    18781 tgatgccgcc gaccacatcc accacgttga agcccatcgg cgtggtgccg atatcccagc
    18841 tccaggtgtc gttgcgccaa ccgaccgcca cgctggcacc ggaatccgac tggctgcggt
    18901 tgccgctaca gtcctgtaat gtacaggtgc cccagttgtc atcccattta ccatcggcat
    18961 tagtggagaa actgccgacg ttcatattga cgaaatcact gcgaaagaac atccgcccgt
    19021 cagaatacgg cgcatccacc tgcaacatgg tagtgtgcgc tttcagatcg gagtaaccac
    19081 cggtgccgct cgaaccccag taatcatgct caagggtgac gttaagatcc tgctggcgat
    19141 agaggtccgc cgcatcgctg cgcacgccgc gtttcagcca gtcatctttc tcgtcattgc
    19201 gggtcagtcg ggtaaaggtg tcgttatcca gcggacgcgt cgtggtcaca ccggatgcaa
    19261 ccatcgcgtc tttgtaggtt tccagcgcct gcgttggatc gcccgcctgc gcttcaaact
    19321 tcgcaccatc acgcagtacc atcgcacttt ccatcgacgg cggctgagat tttgccagcg
    19381 ggatcaactt attaaacgtc tgctgcgccg ctgcggtatc gccaagctgc gcctgtgcca
    19441 gcgccacgcg ccgctgagtg ttcagcgagg cgttatcggt ggcaggcagc ttcgccagct
    19501 ggctacgtgc cgccgctttg tcaccggcag caatatccac ttccgtcaga ccaagaatgg
    19561 catcggcgtt agttggctcc cgcgtcagga cattctgata tgcagcgcga gcggcggtgt
    19621 aatcacgtcg ttgttgcgcc cagtcagcca gcgtgaggtc tatgcgcgtg gaaggtggtt
    19681 gctggcgcag catcgcttcc gcttcagctt ctttgccgct ttctcgcagg cgattagcgg
    19741 tttccaacac ctgatcgctt tgcagtcgat taaccagctc ctgaatattg ctgctccact
    19801 gcgcgcgcgg caggctgttg atatgcgcca gcgccgctct gtcctggtca tgaccagaga
    19861 gatacagccc gtaagcgtaa acctgctccg ggtcgttcgg cttctgctgc gccagattac
    19921 gcattaacgt atcggcctgg ctgcgttgtc cggcctggct gcgttgtccg gcctgccaga
    19981 gatcctgtga aagtcggtaa gtaatccata cactgccggg gtccagcgcc agtcgttgcc
    20041 gctgaagtgc tgccgcctgc gcccatttcc cctggttttc cagtgcctct gcctgctgtg
    20101 ccagacggtc gttttgcagg ctgcgttcga tatcatcaat gctgcgccgc tggctggcag
    20161 agagcgaggc gatatacgct tcggcttttt ctggcgactg ctggcggtaa atattcgcca
    20221 gcccgcgcac ggcgttagtg ttgccgctgt ccatacgcaa ggtctgctgg taatagcgtt
    20281 cggcggcggg ataatctttg cgcgccatcg ccacatcgcc cagcccaagc actgcatagc
    20341 tgtcggtgtt atcgacatta cgtgcctgct ggaacagccg ttctgcccgg tcaggattat
    20401 tggctttcag cgcggcatcg ccctgctgga tcgccagcca gtagcggttt actttcagca
    20461 gactgttcca tttgtcgtta ttgctgttgt gcggatccag cgcgagggct ttttccagat
    20521 tcgccaccgc attagcgcga tcgcctttct gagaatacgc ctggcccagc gcccccagag
    20581 cctcactgtc tttcgggttc gcccgcaccg cctgttgtag ttcgggaatg gctttacccg
    20641 ccatgccaga gtccaccgcc gctaaacctt gcgcacgagc gcggaaagca gggtcggcca
    20701 gctgtttttg ctgttctgcc agttgcgatt gtgcagcagc tacgctatcg ccatcgctaa
    20761 agatcgagag atattttttc agtgcctgaa cgctggcatc gctgacaggc atgtctttaa
    20821 tctgcccgta ccagatttta gaggcccctt cgcgcccggc gttcgatttc gccatctgtt
    20881 ccaggacggc aaaaccttca tcacggcgat cgctactaaa cagtaatagc gccagattgt
    20941 tttgcaggcc cgtgttgccc ggcgtatccg cattaatgcg ttttagctga ttaatcgctt
    21001 cgccacgtcg ggccggaatt ttcgccaccg tactccagta ctcgacagca atgtcacctt
    21061 ccggcggcgc accgttgaac agtttgttgt aactcgccac agcttcttcc gcatgaccag
    21121 tcgtcgcctg caatcttgcc tgttgcagtg cctgacgacc atccgacgtg gaaagcagca
    21181 tcgtagtccg tgacgattta tacgcatttg aactcggcgc taactgcgac agccgatcga
    21241 gctgtttttg tgcgccatca atatcgccct gacgtaacag agaacgaaaa cgggcagcaa
    21301 cgacgtccgg gttattcgga tcaataagtt ccagacgata caacgactgt tgcaccaggt
    21361 cttcacgatg ggtcgcttcg cctaaccgaa cttgctccag caactgttgc tgagcggttg
    21421 gtgctgcctc gaccatcggc atgacggcca gaccgaggga aagcgtgaat atgtttagtg
    21481 tgaatttgcg cattcctggc cccagtcagg taataactca ccttttgtcg agaagcggaa
    21541 acggtgttga tcccagcctt gtccaaacag ggtcagcaca tagttgtaat aggcatcgct
    21601 gccaggaaag ttatcggcca cgcgctggcg ctgaacggcc tgcgcatcgc ggttttgtaa
    21661 aaagggcagc atggcggcag aaaaaccgac cggtccttta ccctgcgctt tccccgtagc
    21721 cacatccact ttttccggcg gataaccgtt tttctcagtg aatgtcgcca tcggtttaaa
    21781 ccggttgagc atccgcgctt tttgcggatc gctgtcaggc atcatgccta cccacatgta
    21841 aacgcggata gcgtcgtagc tgctgatcaa tgttttttcg gcttttagct gccagccttt
    21901 gtctttctca tagcgcaccc agtctggcga aaagcctttc ggggcggttt ccagcaataa
    21961 acgttgattg gtttcgcgca gcgtggtcca cggcgcgcca aagcgggtga aatactgcgc
    22021 cagcgtcggc ggcaggtagc tggggttaaa acgccagctg ttatcctctg caaatcccac
    22081 tttgcccggt aacaacatgg agcccagccc aggcaccgtc accacttcct cccgcgcgat
    22141 acgttttagc aatgcgctgc cgatgtcggt ataacgctgc tctttccaca aacgccccgc
    22201 ctccagcaac gcccaggcca tccagacatc accatcggag gccgaattgc tgtccagcac
    22261 ttcccactta ctgttctctt tcttgcccca cagccaggcg ggcaaatgtt cttttaaaga
    22321 accctgtgcg agattgttct gcgtccagtc gagaagatta tcgaaagctg cacggtcgtt
    22381 agccgccagg gcaaagaaca tgccgtaact ttgcccttcg gaggtggtga ttttgcgcgc
    22441 gtcgctgggg tcgatgacgc gcccttcctg actgatgtaa tcctttttaa actgctccca
    22501 ggcaggccag gtacaggctg cctgaacact aaaggcagcc agcagcagca tcgtcacgag
    22561 tccactacgc aacacattca tcttcaatta ctcgttatcc gggttaagac gacgacggct
    22621 gataatacgc agcagacgcc acagtaccca tgccagcagc acgacgctga ttgccgccag
    22681 caccgccagc agaatcggat ggtttgccag cgcataccat aagcgttcga accacggcag
    22741 atggccaacg taatagacgt cgccaacacg caggctattg ataccggact cgcggatcac
    22801 cgcgaccgaa ccgaacatgg tggcgcgttt gccgctatcg ttcaccgcat cgttaagcat
    22861 ttcataaccg cgtgggctat ctgccagcag cgcaatcacg ctacgctggt cgttatacgg
    22921 cgactggaag ccaatcaccg ctgccatcgc acctgaagag gtcagcgttg actgagtttc
    22981 tgccgcgcga tcgctctcgt ccggtacaat gccggggaac ggggtctggc gcatcggcgt
    23041 tttcacccag ctttcggtcg cctgcaccaa caggtcgatc tgcttgtcgt ctttcagttt
    23101 gtccgggata ccaccgacga tcatgatgtc ggcatctttc ccctgaatag tgctgccatc
    23161 atcggtcacc gtcaggttaa tcgccgggaa gcccgtctgc gcaccgataa agccaacggt
    23221 attcagcaag gtttccatct gtgcttcgtt aggcgctttc ggcatcacgg tgatggtttg
    23281 tgacagatcc gccatccggc tgaacgggaa gcccgcgtta gcaaaggcgc gtagatccgg
    23341 catcgggatg aagtggtaat acttcgagaa gtcgatggtg gaatcatcac caatcaccac
    23401 atgattctgc accggctgga aggtaatgca gttatccacc gaaccgcccg gcatcgggtt
    23461 catatactca aagtcaaagc gcagctggtt ggtcgcgccc agtttcagcg ccggaataga
    23521 gacatccgtt ttgccatcca gcaaaccttg taataccgga atacgcagca gcaggcggtt
    23581 cgcctcctgt ttactgctca ggttgaagga ttgcaggaac tggttattca ggctgatatc
    23641 catccgtgag ctgtctttca ctggcggcat ggtgtagcga taattaatat ccatatcaat
    23701 gccggtactg cgcatcaggt agagatccgg tggcaggttt agcgaaacgt taatcgctgc
    23761 tggctcaaga ccgctggatt gtaactgttc ttcataggtt ttcagttcac caaaggtaac
    23821 cggacgatcg gtacgcaccc agttcggcgc atcgtacggc ttacgcggta gcagcggttt
    23881 cacttcattc actaccacgc tttcaccacg gaacagaatg ttaccctgag cgataccttt
    23941 cgctgcctgc aacaggtctt tgtcgtcacg accaaacacc accagcagtt tgacataagg
    24001 attctgcgga tggttgatca tttcaatcac cggggctttt accgccggat gatcgcgcag
    24061 gaagtccggg cgtttgtcat tggtggcaaa aacgatggca ttgcgatccg gcaattggtt
    24121 atagagcacc gggaagttct gcccacgcca gccagaacgc gaaccaaacc acgaggcgac
    24181 aatggcagat gcttgttgca gcccgacatc cggcgcaccc gcaaagacca tcggcaaggt
    24241 gttagtacgg ttatcacgcg ggtcaaagaa tggcaccggg aagtgtgaca ggtcattctt
    24301 cacattcagg gtctgatagg tcagatccag tccactgctc cgcccaacat ccagccaaag
    24361 cgtggtgctg gccgggtttt cgcacacgtc ctgataatga ccgacaaact ccagccgcac
    24421 acggttgaag tcggtaataa acagtgggtt aattggcatt tgtgccagcg tttttttacc
    24481 caactgttct ttggtcactg gcagcacgcc catcagttca tcattgagat aaacctttaa
    24541 ctgcgactgg acaggcagta atgatggcga tggcgtgtat tcgaggttga gcatcgcctt
    24601 cgtcaccact tcatcgctgc gcataccaaa ctcaatgctg ccgttcgggt taatgccacg
    24661 tagcaccatg ctgcccggcg gcggcgcaat ttgcgcaaag gtcagcttca catcacggga
    24721 aggaccgttc tgcgccacga ctggcgcatc tgcgccctgc acgcccggca tcacttgccc
    24781 cacctgcgga ttttgctccg cctgggcggc tacagctggc tcagcattga tcagtggttg
    24841 cgttgctggc gtcgcctgcg tcatgaaaga ggggaacgca ctcatcccca gagccactgc
    24901 acaaatccag aatatttttc ttttcatcgc gttatcatca ttgttgagcc aaagcctgat
    24961 ccgatggttg tgccgtttcg ctccgctccg ggcggcgcgg aataaacgat acaacccagg
    25021 aaaccagaga agtcagcaca cggaatatgc ccttcaccga agaaggcgca aattccgcca
    25081 gatggcggta gccacggaag ccgagcttca gaatatccag cagactttcc agcggcttat
    25141 cttccgggta gctgtcctgc cagagcgccc atgtatccgc acgggcaaac gtacactgca
    25201 caaaatcgat atgttgctgg gtagtgagcg gcattaattt cagcccaact tcattaccca
    25261 tcacacgcgc cacctgagcc gggaagacgt attcctgctg accgcgttta agcaacagat
    25321 tcactttctg cccttccaga atctgcgcct gaccgttgat cttgatcccc aaaccaccgt
    25381 cggagaaatc ctgaacggta cacgagaaga ggtgaccatc ttcgcgggca attgctgcgg
    25441 gcatcgtcat ctccacgcgg tgcgatcggc gtacctgttt gctttctacc gatactgcga
    25501 ctgcgccgcc gagaataatc aggttgtaga atacccacac catactgacg accacggtga
    25561 gcatctcggt tggcggacca tagaagtagc gccagatccc aaccgctacg cccaccaggt
    25621 tgagcagaac aaggaagatg tagggccgcg agatcaccca gtcgacgtac tcttcttcca
    25681 ccagtccacc tttggcggtg acgttaaatt tgcctttgtg cgggttaatc agcgccacca
    25741 gcgtcggtgg tgcgatatac cacgccagca ctgtttcgta gatttcactc cagaaagagt
    25801 ggcgatattt gccctggatc ttcgagttag tcaggctggc atggatcata tgcggtagca
    25861 cgaacagggc gatcatcaac gctggcgcgt agatgatgta ggcatgaaac agcaggaacg
    25921 ccagcggcgc agtcaggaag atcagccgtg gaatgcccga caagaaatgg aacatggcgt
    25981 tgacatagca cagccgctga gcaaacttca gccctttacc ggtgagtggg ttatcgagac
    26041 ggaagatttg caccatcccg cgcgcccagc gaatacgctg accgatatgc gccgagagac
    26101 tttcggtcgc cagccccgct gcctgcggaa tacgcatata cgctgaggtg tagccacgac
    26161 ggtgcagacg cagagaagtg tgcgcatctt cggttaccgt ttcgacagca ataccgccaa
    26221 tctcatccag cggcttacgg cgaatcaccg cacatgaacc gcagaagaaa gtggcatccc
    26281 acatatcatt gccgtcctgc accagaccat agaacagcgt gccttcgttc ggcgttttac
    26341 gaaaacgccc caggttgcgt tcaaacggat ccggcgagaa gaagtggtgc ggcgtctgca
    26401 tcatcgccag ctgtttctct ttcaggaacc agcccatggt catttgcagg aacgatcgcg
    26461 ttggtacgtg gtcgcagtcg aatatcgaca cgaactcgcc tttggcatat ttcagcgcat
    26521 tgttgatgtt gcccgctttc gcatgttcat gagtggtacg ggcgatatat ttcaccccca
    26581 cgttttgcgc aaactggcga aactcttccc tgccgccgtc atcgaggatc cagatgttca
    26641 gcttgtcttt cggccagtcg atacccagcg aagcgtaaat ggtatttttc accacgttga
    26701 gatcttcgtt gtaagtcggg acaaagatat ccaccgacgg ccacagcgac atatctttcg
    26761 gcaatggcac cggctgacga ttcagcggcc atacgacctg gaagtagccg agcaccagca
    26821 ctatccacgc gtacgtttcg gcgaacagca gaataagccc gcacaccagg ctgaccggat
    26881 cgtcccagtt taacgtcgag gtgtaacgcc accagatata acggcaagaa acggtcagcg
    26941 acagcacaat cagcatcagc gccgagaagc gccccggcat ccgccgtacg atcagcgcta
    27001 ccccccacag cagcatcagg aagataaact gcgctagcgg gttaaacggc tgagtaacgc
    27061 agattaacgc cagaatcagc gagaaggtga cgatgatacc aaggatcaac cgccgcgccc
    27121 cggcactcaa atgaccgagc tcttttttct catcaagatg ctgtgtttta tggctaacgc
    27181 gctcaggcag ctcgttcatc cattgatggt aacgtccacg aatattttgt agacctgaaa
    27241 atgcccgcct gcgcggtttc ggcgtttctt tgcgcgatgc accgatcagt aaccagcatg
    27301 tttgaataag ataacggacc gggtccagcg gacgcggacg cgaggcgttg atatgcggat
    27361 acaggttttt atgttctgcg cgaatacgct gccagcgcgg gtgctccagc ggaataaaaa
    27421 tccaggccag gatcatccag aaacagccga gcgtcgcgct gaaagccgac gcaccgtgac
    27481 gacgataatc gcgataacgc ccaataagcc gtgcgttgac cggcgggata agcaaccacc
    27541 gggtcaggat actcatgatg cactcccgac tggcgttttc agcccggaat aattcaacag
    27601 gcaccagttc gccagcgtca gtatctcttc agccgccagc gcatcactgc gatattcacc
    27661 gactggttgc ttagctgcca gacattcagc catcgcttca tcacgatgaa tgagcatcgg
    27721 cagcaatcgg cgctggcttt gcaaccaaag ctggtaaata tcgtcctgaa cctggctgcc
    27781 aatacggaag tcattaatca aaatatgtgc accatccggc agcgcttgct gatgcagtcg
    27841 gatatggcag ttggcatcca cattgacgat tgccagcgag tgatcgcaca aactcagcag
    27901 ctggtgggtt atctgcgagg catcacgcgg taagtcaatt aaaatccact ggtaacgccc
    27961 gctggctttt agttgctgta agccggtgca aatatcgctc agccgggttt gccagtgctg
    28021 tggattttct tgttcttcaa tcgataactg accaaaaggc agcaaatcga gctgcgaggt
    28081 atagcgcaac ccggcgtcac gccagtcctg gccatccagc atagctctgg cccaaccctg
    28141 acggtgggta aaatcaacgt taaatgacag gcgcaacaag ttgtccgggc aggcatcgac
    28201 caccaggaca ttttctccca acatttgtaa tgaccaggct aatgcggcgg tga
//
LOCUS       DAZFLD010000042        27728 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.042, whole genome shotgun sequence.
ACCESSION   DAZFLD010000042 DAZFLD010000000
VERSION     DAZFLD010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27728
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.042"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            196..1767
                     /gene="garD"
                     /locus_tag="ACHKBL_001513"
     CDS             196..1767
                     /gene="garD"
                     /locus_tag="ACHKBL_001513"
                     /EC_number="4.2.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008867 - galactarate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0046392 - galactarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate dehydratase"
                     /protein_id="HFO5818398.1"
                     /translation="MANIEIRQETPTAFYIKVHDTDNVAIIVNDNGLKAGTRFPDGLE
                     LIEHIPQGHKVALLDIPANGEIIRYGEVIGYAVRAIPRGSWIDESMVVLPEAPPLHTL
                     PLATKVPAPLPPLEGYTFEGYRNADGSVGTKNLLGITTSVHCVAGVVDYVVKIIERDL
                     LPKYPNVDGVVGLNHLYGCGVAINAPAAVVPIRTIHNISLNPNFGGEVMVIGLGCEKL
                     QPERLLVGTDDVQGIPLENASIVSLQDEKHVGFQSMVEDILQVAERHLHKLNQRQRET
                     CPASELVVGMQCGGSDAFSGVTANPAVGYASDLLVRCGATVMFSEVTEVRDAIHLLTP
                     RAVNEEVGKRLLEEMEWYDNYLNMGKTDRSANPSPGNKKGGLANVVEKALGSIAKSGK
                     SAIVEVLSPGQRPTKRGLIYAATPASDFVCGTQQVASGITVQVFTTGRGTPYGLMAIP
                     VIKMATRTELANRWFDLMDINAGTIATGEETIEEVGWKLFHFILDVASGKKKTFSDQW
                     GLHNQLAVFNPAPVT"
     gene            1916..2251
                     /gene="prlF"
                     /locus_tag="ACHKBL_001514"
     CDS             1916..2251
                     /gene="prlF"
                     /locus_tag="ACHKBL_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin PrlF"
                     /protein_id="HFO5818399.1"
                     /translation="MPANARSNAVLTTESKVTIRGQTTIPAPVREALKLKPGLDSIHY
                     EILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNNPQKTRPFDIQQGKKLVAGMDVN
                     IDDEIGDDE"
     gene            2251..2715
                     /gene="yhaV"
                     /locus_tag="ACHKBL_001515"
     CDS             2251..2715
                     /gene="yhaV"
                     /locus_tag="ACHKBL_001515"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ribonuclease
                     toxin YhaV"
                     /protein_id="HFO5818400.1"
                     /translation="MDFPQRVNGWALYAHPCFQETYDALVAEVEALKGKDPENYQRKA
                     ATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKNKDWSRVKFGAGRYRLFFRYSEK
                     EKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH"
     gene            complement(2770..3579)
                     /gene="agaR"
                     /locus_tag="ACHKBL_001516"
     CDS             complement(2770..3579)
                     /gene="agaR"
                     /locus_tag="ACHKBL_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aga operon transcriptional regulator AgaR"
                     /protein_id="HFO5818401.1"
                     /translation="MSNTDASGEKRVTGTSERREQIIQRLRQQGSVQVNDLSALYGVS
                     TVTIRNDLAFLEKQGIAVRAYGGALICDSTTPSVEPSVEDKSALNTAMKRSVAKAAVE
                     LIQPGHRVILDSGTTTFEIARLMRKHTDVIAMTNGMNVANALLEAEGVELLMTGGHLR
                     RQSQSFYGDQAEQSLQNYHFDMLFLGVDAIDLERGVSTHNEDEARLNRRMCEVAERII
                     VVTDSSKFNRSSLHKIIDTQRIDMIIVDEGIPADSLEGLRKAGVEVILVGE"
     gene            3828..5108
                     /gene="kbaZ"
                     /locus_tag="ACHKBL_001517"
     CDS             3828..5108
                     /gene="kbaZ"
                     /locus_tag="ACHKBL_001517"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaZ"
                     /protein_id="HFO5818402.1"
                     /translation="MKHLTEMVRQHKAGKTNGIYAVCSAHPLVLEAAIRYASANQTPL
                     LIEATSNQVDQFGGYTGMTPADFRGFVCQLADSLNFPQDALILGGDHLGPNRWQNLPA
                     AQAMANADDLIKSYVAAGFKKIHLDCSMSCQDDPIPLTDGIVAERAARLAKVAEETCL
                     EHFGEADLVYVIGTEVPVPGGAHETLSELAVTTPDAARATLEAHRHAFEKQGLSAIWP
                     RIIALVVQPGVEFDHTNVIDYQPVKATALSQMVENYETLIFEAHSTDYQTPQSLRQLV
                     IDHFAILKVGPALTFALREALFSLAAIEEELVPAKACSGLRQVLENVMLDRPEYWQSH
                     YHGDGNARRLARGYSYSDRVRYYWPDSQIDDAFAHLVRNLADSPIPLPLISQYLPLQY
                     VKVRSGELQPTPRELIINHIQDILAQYHTACEGQ"
     gene            5131..5604
                     /gene="agaV"
                     /locus_tag="ACHKBL_001518"
     CDS             5131..5604
                     /gene="agaV"
                     /locus_tag="ACHKBL_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIB"
                     /protein_id="HFO5818403.1"
                     /translation="MPNIVLSRIDERLIHGQVGVQWVGFAGANLVLVANDEVAEDPVQ
                     QNLMEMVLAEGIAVRFWTLQKVIDNIHRAADRQKILLVCKTPADFLTLVKGGVPVNRI
                     NVGNMHYANGKQQIAKTVSVDAGDIAAFNDLKAAGVECFVQGVPTEPAVDLFKLL"
     gene            5615..6394
                     /gene="agaW"
                     /locus_tag="ACHKBL_001519"
     CDS             5615..6394
                     /gene="agaW"
                     /locus_tag="ACHKBL_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIC"
                     /protein_id="HFO5818404.1"
                     /translation="MEISLLQAFALGIIAFIAGLDMFNGLTHMHRPVVLGPLVGLVLG
                     DLHTGILTGGTLELVWMGLAPLAGAQPPNVIIGTIVGTAFAITTGVKPDVAVGVAVPF
                     AVAVQMGITFLFSVMSGVMSRCDRMAENADTRGIERVNYLALLALGTFYFLCAFLPIY
                     FGAEHAKTIIDVLPQRLIDGLGVAGGIMPAIGFAVLLKIMMKNVYIPYFILGFVAAAW
                     LKLPVLAIAAAALAMALIDLLRKSPEPTQPAAQKEEFEDGI"
     gene            6384..7262
                     /gene="agaE"
                     /locus_tag="ACHKBL_001520"
     CDS             6384..7262
                     /gene="agaE"
                     /locus_tag="ACHKBL_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019079236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IID"
                     /protein_id="HFO5818405.1"
                     /translation="MASNQTTLPNVSENEETLLTGVNENVYEDQSIGAELTKKDINRV
                     AWRSMLLQASFNYERMQASGWLYGLLPALKKIHTNKRDLARAMKGHMGFFNTHPFLVT
                     FVIGIILAMERSKQDVNSIQSTKIAVGAPLGGIGDAMFWLTLLPICGGIGASLALQGS
                     ILGAVVFIVLFNVVHLGLRFGLAHYAYRMGVAAIPLIKANTKKVGHAASIVGMTVIGA
                     LVATYVRLSTTLEITAGDAVVKLQADVIDKLMPAFLPLVYTLTMFWLVRRGWSPLRLI
                     AVTVVLGIVGKFCHFL"
     gene            7280..7714
                     /gene="agaF"
                     /locus_tag="ACHKBL_001521"
     CDS             7280..7714
                     /gene="agaF"
                     /locus_tag="ACHKBL_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine/N-acetylgalactosamine
                     transporter subunit IIA"
                     /protein_id="HFO5818406.1"
                     /translation="MLSIILTGHGGFASGMEKAMKQILGEQSQFIAIDFPETSSTALL
                     TSQLEEAIAQLDCEDGIVFLTDLLGGTPFRVASTLAMQKLGCEVITGTNLQLLLEMVL
                     EREGLSGEEFRVQALECGHRGLTSLVDELGRCHEECPVEEGI"
     gene            7711..8844
                     /gene="nagA"
                     /locus_tag="ACHKBL_001522"
     CDS             7711..8844
                     /gene="nagA"
                     /locus_tag="ACHKBL_001522"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFO5818407.1"
                     /translation="MTPVLRARRLLTEEGWLDDHQLRIADGVIAEIEPIPVGVTERDA
                     ELLCPAYIDTHVHGGAGVDVMDDAPDVLDKLAMHKAREGVGSWLPTTVTAPLNTIHAA
                     LERIAQRCQRGGPGAQILGSYLEGPYFTPQNKGAHPPELFRELEIAELDQLIAVSQHT
                     LRVVALAPEKEGALQAIRHLKQQNVRVMLGHSAATWQQTRAAFDAGADGLVHCYNGMT
                     GLHHREPGMVGAGLTDKRAWLELIADGHHVHPAAMSLCCCCAKERIVLITDAMQAAGM
                     PDGRYTLCGEEVQMHGGVVRTASGGLAGSTLSVDAAVRNMVELTGVTPAEAIHMASLH
                     PARMLGVDDVLGSLKPGKRASVVALDSGLHAQQIWIQGQLASF"
     gene            9194..10348
                     /locus_tag="ACHKBL_001523"
     CDS             9194..10348
                     /locus_tag="ACHKBL_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgaS family sugar isomerase"
                     /protein_id="HFO5818408.1"
                     /translation="MPENYTPAAAATGTWTEEEIRHQPRAWIRSLTNIDALRSALNNF
                     LEPLLRKENLQIILTGAGTSAFIGDIIAPWLASHTGKNFSAVPTTDLVTNPMDYLNPA
                     HPLLLISFGRSGNSPESVAAVELANQFVPECYHLPITCNEAGALYQNAINSDNAFALL
                     MPAETHDRGFAMTSSITTMMASCLAVFAPETINSQSFRDVADRCQAILTSLGDFSEGV
                     FGYAPWKRIVYLGSGGLQGAARESALKVLELTAGKLAAFYDSPTGFRHGPKSLVDNET
                     LVVVFVSSHPYTRQYDLDLLAELRRDNQAMRVIAIAAESNDVITAGPHIILPPSRHFI
                     DVEQAFCFLMYAQTFALMQSLHMGNTPDTPSASGTVNRVVQGVIIHPWQA"
     gene            10361..11221
                     /gene="kbaY"
                     /locus_tag="ACHKBL_001524"
     CDS             10361..11221
                     /gene="kbaY"
                     /locus_tag="ACHKBL_001524"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaY"
                     /protein_id="HFO5818409.1"
                     /translation="MSIISTKYLLQDAQANGYAVPAFNIHNAETIQAILEVCSEMRSP
                     VILAGTPGTFKHIALEEIYALCSAYSTTYNMPLALHLDHHESLDDIRRKVHAGVRSAM
                     IDGSHFPFAENVKLVKSVVDFCHSQDCSVEAELGRLGGVEDDMSVDAESAFLTDPQEA
                     KRFVELTGVDSLAVAIGTAHGLYSKTPKIDFQRLAEIREVVDVPLVLHGASDVPDEFV
                     RRTIELGVTKVNVATELKIAFAGAVKAWFAENPQGNDPRYYMRVGMDAMKEVVRNKIN
                     VCGSANRISA"
     gene            11388..11864
                     /gene="agaB"
                     /locus_tag="ACHKBL_001525"
     CDS             11388..11864
                     /gene="agaB"
                     /locus_tag="ACHKBL_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine transporter subunit IIB"
                     /protein_id="HFO5818410.1"
                     /translation="MSSPNILLTRIDNRLVHGQVGVTWTSTIGANLLVVVDDVVANDD
                     IQQKLMSITAETYGFGIRFFTIEKTINVIGKAAPHQKIFLICRTPQTVRKLVEGGIDL
                     KDVNVGNMHFSEGKKQISSKVYVDDQDLTDLRFIKQRGVNVFIQDVPGDQKEQIPD"
     gene            11903..12706
                     /gene="agaC"
                     /locus_tag="ACHKBL_001526"
     CDS             11903..12706
                     /gene="agaC"
                     /locus_tag="ACHKBL_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine transporter subunit IIC"
                     /protein_id="HFO5818411.1"
                     /translation="MHEITLLQGLSLAALVFVLGIDFWLEALFLFRPIIVCTLTGAIL
                     GDIQTGLITGGLTELAFAGLTPAGGVQPPNPIMAGLMTTVIAWSTGVDAKTAIGLGLP
                     FSLLMQYVILFFYSAFSLFMTKADKCAKEADTAAFSRLNWTTMLIVASAYAVIAFLCT
                     YLAQGAMQALVKAMPAWLTHGFEVAGGILPAVGFGLLLRVMFKAQYIPYLIAGFLFVC
                     YIQVSNLLPVAVLGAGFAVYEFFNAKSRQQAQPQPVASKNEEEDYSNGI"
     gene            12696..13487
                     /gene="agaD"
                     /locus_tag="ACHKBL_001527"
     CDS             12696..13487
                     /gene="agaD"
                     /locus_tag="ACHKBL_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine transporter subunit IID"
                     /protein_id="HFO5818412.1"
                     /translation="MGSEISKKDITRLGFRSSLLQASFNYERMQAGGFTWAMLPLLKK
                     IYKDDKPGLSAAMKDNLEFINTHPNLVGFLMGLLISMEEKGENRDTIKGLKVALFGPI
                     AGIGDAIFWFTLLPIMAGICSSFASQGNLLGPILFFAVYLLIFFLRVGWTHIGYSVGV
                     KAIDKVRENSQMIARSATILGITVIGGLIASYVHINVVTSFAIDSTHSVALQQDFFDK
                     VFPNILPMAYTLLMYYFLRVKKAHPVLLIGVTFVLSIVCSAFGIL"
     gene            13488..14255
                     /locus_tag="ACHKBL_001528"
     CDS             13488..14255
                     /locus_tag="ACHKBL_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019843039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactosamine-6-phosphate isomerase"
                     /protein_id="HFO5818413.1"
                     /translation="MERGTASGGASLLKEFHPVQTLQQVENYTALSERASEYLLAVIR
                     SKPDAVICLATGATPLLTYHYLVEKIHQQQVDVSQLTFVKLDEWVDLPLTMPGTCETF
                     LQQHIVQPLGLREDQLISFRSEEINETECERVTNLIARKGGLDLCVLGLGKNGHLGLN
                     EPGESLQPACHISQLDARTQQHEMLKTAGRPVTRGITLGLKDILNAREVLLLVTGEGK
                     QDATERFLTAKVSTAIPASFLWLHNYFTCMIDEMCRR"
     gene            complement(14305..15168)
                     /gene="rsmI"
                     /locus_tag="ACHKBL_001529"
     CDS             complement(14305..15168)
                     /gene="rsmI"
                     /locus_tag="ACHKBL_001529"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000809248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HFO5818414.1"
                     /translation="MKQHQSADNSQGQLYIVPTPIGNLADITQRALEVLQAVDLIAAE
                     DTRHTGLLLQHFGINARLFALHDHNEQQKAETLLAKLQEGQNIALVSDAGTPLINDPG
                     YHLVRTCREAGIRVVPLPGPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALKAIE
                     AEPRTLIFYESTHRLLDSLEDIVAVLGESRYVVLARELTKTWETIHGAPVGELLAWVK
                     EDENRRKGEMVLIVEGHKAQEEDLPADALRTLALLQAELPLKKAAALAAEIHGVKKNA
                     LYKYALEQQGE"
     gene            15233..17269
                     /gene="lpoA"
                     /locus_tag="ACHKBL_001530"
     CDS             15233..17269
                     /gene="lpoA"
                     /locus_tag="ACHKBL_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoA"
                     /protein_id="HFO5818415.1"
                     /translation="MVPSTFSRLKAARCLPVVLAALIFAGCGTHTPDQSTAYMQGTAQ
                     ADSAFYLQQMQQSSDDTRINWQLLAIRALVKEGKTGQAVELFNQLPQELNDSQRREKT
                     LLAAEIKLAQKDFAGAQNLLAKITPADLEQNQQARYWQAKIDASQGRPSIDLLRALIA
                     QEPLLGAKEKQQNIDATWQALSSMTQEQANTLVINADENILQGWLDLQRVWFDNRNDP
                     DMMKAGIADWQKRYPNNPGAKMLPTQLVNVKAFKPASTNKIALLLPLNGQAAVFGRTI
                     QQGFEAAKNIGTQPVAAQVAAAPAADVAEQPQPQTVDGVASPAQASVSELTGEQPAAQ
                     PVPVSAPATSTAAVSAPANPSAELKIYDTSSQPLSQILSQVQQDGASIVVGPLLKNNV
                     EELLKSNTPLNVLALNQPENIENRVNICYFALSPEDEARDAARHIRDQGKQAPLVLIP
                     RSSLGDRVANAFAQEWQKLGGGTVLQQKFGSTSELRAGVNGGSGIALTGSPITPRATT
                     DSGMTTNNPTLQTTPTDDQFTNNGGRVDAVYIVATPGEIAFIKPMIAMRNGSQSGATL
                     YASSRSAQGTAGPDFRLEMEGLQYSEIPMLAGGNLPLMQQALSAVNNDYSLARMYAMG
                     VDAWSLANHFSQMRQVQGFEINGNTGSLTANPDCVINRKLSWLQYQQGQVVPAS"
     gene            17227..17622
                     /locus_tag="ACHKBL_001531"
     CDS             17227..17622
                     /locus_tag="ACHKBL_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HFO5818416.1"
                     /translation="MATVPTRSGSPRQLTTKQTGDVWEAQARRWLEGKGLRFIAANVN
                     ERGGEIDLIMREGRITVFVEVRYRRSALYGGAAASVTRSKQHKLLQTARLWLARHNGS
                     FDTVDCRFDVVAFTGNEVEWIKDAFNDHS"
     gene            17642..18232
                     /gene="diaA"
                     /locus_tag="ACHKBL_001532"
     CDS             17642..18232
                     /gene="diaA"
                     /locus_tag="ACHKBL_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001483830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA initiator-associating protein DiaA"
                     /protein_id="HFO5818417.1"
                     /translation="MQERIKACFTESIQTQIAAAEALPDAISRAAMTLVQSLLNGNKI
                     LCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIANDRLHDEVYAKQ
                     VRALGHAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVALTGYDGGELAGLLGPQDVE
                     IRIPSHRSARIQEMHMLTVNCLCDLIDNTLFPHQDV"
     gene            18242..18817
                     /gene="dolP"
                     /locus_tag="ACHKBL_001533"
     CDS             18242..18817
                     /gene="dolP"
                     /locus_tag="ACHKBL_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006970 - response to osmotic stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/outer membrane stress-associated
                     lipid-binding lipoprotein"
                     /protein_id="HFO5818418.1"
                     /translation="MKALSPIAVLLSALLLQGCVAAAVVGTAAVGTKAATDPRSVGTQ
                     VDDGTLEVRVNSALSKDEQIKKEARINVTAYQGKVLLVGQSPNAELSARAKQIAMGVD
                     GANEVYNEIRQGQPIGLGEASNDTWITTKVRSQLLTSDLVKSSNVKVTTENGEVFLMG
                     LVTEREAKAAADIASRVSGVKRVTTAFTFIK"
     gene            complement(18930..19970)
                     /locus_tag="ACHKBL_001534"
     CDS             complement(18930..19970)
                     /locus_tag="ACHKBL_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFO5818419.1"
                     /translation="MTGQSSSQAATPIQWWKPALFFLVVIAGLWYVKWQPYYGKAFTA
                     AETHSIGKSILAQADANPWQAALDYAMIYFLAVWKAAVLGVLLGSLIQVLIPRDWLLR
                     TLGQSRFRGTLLGTLFSLPGMMCTCCAAPVAAGMRRQQVSMGGALAFWMGNPLLNPAT
                     LVFMGFVLGWGFAAIRLVAGLVMVLLIATLVQKWVRETPQTQAPVEIDIPEAQGGFFS
                     RWGRALWTLFWSTIPVYILAVLVLGAARVWLFPHADGAVDNSLMWVVAMAVAGCLFVI
                     PTAAEIPIVQTMMLAGMGTAPALALLMTLPAVSLPSLIMLRKAFPAKALWLTGAMVAV
                     SGVIVGGLALLV"
     gene            complement(20043..20678)
                     /locus_tag="ACHKBL_001535"
     CDS             complement(20043..20678)
                     /locus_tag="ACHKBL_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HFO5818420.1"
                     /translation="MSQVLITGATGLVGGHLLRMLINEPKVNAIAAPTRRPLGDMPGV
                     FNPHDPQLTDALAQVTDPIDIVFCCLGTTRREAGSKEAFIHADYTLVVDTALTGRRLG
                     AQHMLVVSAMGANAHSPFFYNRVKGEMEEALIAQNWPKLTIARPSMLLGDRSKQRMNE
                     TLFAPLFRLLPGNWKSIDARDVARVMLAEAMRPEHEGVTILSSSELRKRAE"
     gene            20806..21324
                     /gene="yhbO"
                     /locus_tag="ACHKBL_001536"
     CDS             20806..21324
                     /gene="yhbO"
                     /locus_tag="ACHKBL_001536"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein/nucleic acid deglycase"
                     /protein_id="HFO5818421.1"
                     /translation="MSKKIAVLITDEFEDSEFTSPADEFRKAGHEVITIEKQAGKTVK
                     GKKGEASVAIDKSIDEVTPAEFDALLLPGGHSPDYLRGDNRFVTFTRDFVNSGKPVFA
                     ICHGPQLLISADVIRGRKLTAVKPIIIDVKNAGAEFYDQEVVVDKDQLVTSRTPDDLP
                     AFNREALRLLGA"
     gene            complement(21304..21747)
                     /locus_tag="ACHKBL_001537"
     CDS             complement(21304..21747)
                     /locus_tag="ACHKBL_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbP family protein"
                     /protein_id="HFO5818422.1"
                     /translation="METLTAISRWLAKQHVVTWCVQQEGELWCANAFYLFDAQKVAFY
                     ILTEEKTRHAQMSGPQAAVAGTVNGQPKTVALIRGVQFKGEIRRLEGEESDLARQAYN
                     RRFPVARMLSAPVWEIRLDEIKFTDNTLGFGKKMIWLRNSGTEQA"
     gene            21798..22100
                     /gene="yhbQ"
                     /locus_tag="ACHKBL_001538"
     CDS             21798..22100
                     /gene="yhbQ"
                     /locus_tag="ACHKBL_001538"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage response exodeoxyribonuclease YhbQ"
                     /protein_id="HFO5818423.1"
                     /translation="MTPWFLYLIRTADDKLYTGITTDVERRYQQHQSGKGAKALRGKG
                     ELTLAFSAPVGDRSLALRAEYRVKQLTKRQKERLVAEGAGFAELLSSLQTPKIKSD"
     gene            complement(22087..22590)
                     /locus_tag="ACHKBL_001539"
     CDS             complement(22087..22590)
                     /locus_tag="ACHKBL_001539"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO5818424.1"
                     /translation="MLIRVEIPIDAPGIDALLRRSFESDAEAKLVHDLREDGFLTLGL
                     VATDDEGQVIGYVAFSPVDVQGEDLQWVGMAPLAVDEKYRGQGLARQLVYEGLDSLNE
                     FGYAAVVTLGDPALYSRFGFELAAHHDLRCRWPGTESAFQVHRLADDALNGVTGLVEY
                     HEHFNRF"
     gene            complement(22584..23108)
                     /locus_tag="ACHKBL_001540"
     CDS             complement(22584..23108)
                     /locus_tag="ACHKBL_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HFO5818425.1"
                     /translation="MLDKLRSRIVHLGPSLLSVPVKLTPFALKRQVLEQVLSWQFRQA
                     LDDGELEFLEGRWLSIHVRDIDLQWFTSVVNGKLVVSQNAQADVSFSADASDLLMIAA
                     RKQDPDTLFFQRRLVIEGDTELGLYVKNLMDAIELEQMPKALRMMLLQLADFVEAGMK
                     TAPETKQTSVGEPC"
     gene            23317..24312
                     /locus_tag="ACHKBL_001541"
     CDS             23317..24312
                     /locus_tag="ACHKBL_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HFO5818426.1"
                     /translation="MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTE
                     KKLQEAVSFVHQHRRKLHIAINTFAHPDGYARWQRAVDMAAQLGADALILADLAMLEY
                     AAERYPHIERHVSVQASATNEEAINFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPL
                     EVFAFGSLCIMSEGRCYLSSYLTGESPNTVGACSPARFVRWQQTPQGLESRLNEVLID
                     RYQDGENAGYPTLCKGRYLVDGERYHALEEPTSLNTLELLPELMAANIASVKIEGRQR
                     SPAYVSQVAKVWRQAIDRCKADPQNFVPQSAWMETLGSMSEGTQTTLGAYHRKWQ"
     gene            24321..25199
                     /locus_tag="ACHKBL_001542"
     CDS             24321..25199
                     /locus_tag="ACHKBL_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HFO5818427.1"
                     /translation="MKYSLGPVLWYWPKETLEDFYQQAAASSADVIYLGEAVCSKRRA
                     TKVGDWLEMAKSLAGSGKQIVLSTLALVQASSELGELKRYVENGEFLIEASDLGVVNM
                     CAERKLPFVAGHALNCYNAVTLKILLKQGMMRWCMPVELSRDWLVNLLNQCDELGIRN
                     QFEVEVLSYGHLPLAYSARCFTARSEDRPKDECETCCIKYPNGRNVLSQENQQVFVLN
                     GIQTMSGYVYNLGNELASMQGLVDVVRLSPQGTDTFAILDAFRANENGAAPLPLTANS
                     DCNGYWRRLAGLELQA"
     gene            25280..26287
                     /locus_tag="ACHKBL_001543"
     CDS             25280..26287
                     /locus_tag="ACHKBL_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="luciferase-like monooxygenase"
                     /protein_id="HFO5818428.1"
                     /translation="MTDKTIAFSLLDLAPIPEGSSAREAFSHSLDLARLAEKRGYHRY
                     WLAEHHNMTGIASAATSVLIGYLAANTTTLHLGSGGVMLPNHSPLVIAEQFGTLNTLY
                     PGRIDLGLGRAPGSDQRTMMALRRHMSGDIDNFPRDVAELVDWFDARDPNPHVRPVPG
                     YGEKIPVWLLGSSLYSAQLAAQLGLPFAFASHFAPDMLFQALHLYRSNFKPSARLEKP
                     YAMVCINIIAADSNRDAEFLFTSMQQAFVKLRRGETGQLPPPIQNMDQFWSPSEQYGV
                     QQALSMSLVGDKAKVRHGLQSILRETDADEIMVNGQIFDHQARLHSFELAMDVKEELL
                     G"
     gene            complement(26405..27649)
                     /gene="mtr"
                     /locus_tag="ACHKBL_001544"
     CDS             complement(26405..27649)
                     /gene="mtr"
                     /locus_tag="ACHKBL_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015173 - aromatic amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015801 - aromatic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HFO5818429.1"
                     /translation="MATLTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWS
                     MAALIFTWFCMLHSGLMILEANLNYRIGSSFDTITKDLLGKGWNVVNGISIAFVLYIL
                     TYAYISASGSILHHTFAEMSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAK
                     VITFFLTFGSLLGHVQPTTLFNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYY
                     GKDPKTIVKCLVYGTLMALALYTIWLLATMGNIPRPEFIGIAEKGGNIDVLVQALSGV
                     LNSRSLDLLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSAMGRLKTALLTFAPPV
                     VGGLLFPNGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPKFRVWGGKPMIMLIL
                     VFGVGNALVHILSSFNLLPVYQ"
BASE COUNT         6729 a         7338 c         7269 g         6392 t
ORIGIN      
        1 cacataaggt tgcgcgctaa agcacagatt tgcgctttac cttgccgggc ggcactgcaa
       61 tccctgaaat gattgacatt gatcacattt ctgcatttaa actcctgaca ttcttatttc
      121 acccaatgaa gttatttatt tttaaatgag accaggtcct cattttaata acccctggct
      181 ggagaatatt gcacaatggc caacatcgaa atcagacaag aaacgccaac tgcgttttat
      241 ataaaagttc acgacacaga taatgtggca attattgtta atgataatgg cctgaaagca
      301 ggaacgcgtt ttccggatgg gctggaatta attgaacata ttccccaggg gcataaagtc
      361 gcattgctgg acattccggc taatggtgaa attattcgtt atggcgaagt gattggttac
      421 gccgtgcgcg caatcccacg cggaagctgg atcgacgaat caatggttgt actaccggaa
      481 gcgccgccgt tacacacgct gccactggca accaaagtcc cggcaccctt accgccactg
      541 gaaggataca cctttgaggg ctatcgcaat gccgatggca gcgtgggcac caaaaacctg
      601 ctcggcatca ccaccagcgt ccactgtgtg gcaggcgtgg tggactatgt agtaaaaatc
      661 attgaacgcg atctgctgcc gaaatacccg aacgtcgatg gcgtggtggg gctgaatcat
      721 ctgtacggtt gtggcgtggc gattaacgct ccggcggcgg tggtgcccat tcggaccatt
      781 cacaacattt cgcttaaccc aaactttggc ggcgaggtga tggtgattgg cctgggttgc
      841 gagaaattac agccagaacg cctgctggtc ggcacggatg atgtacaagg tattccgctg
      901 gagaacgcca gtattgtcag cttgcaggat gaaaagcatg tcggttttca gtccatggtc
      961 gaggatattt tgcaggtcgc cgaacgccat ttgcacaaac tgaatcaacg gcagcgagaa
     1021 acctgtccag cttcagaact ggttgtcggc atgcagtgcg gtggcagcga tgcgttttct
     1081 ggcgtaacgg caaacccggc ggttgggtat gcgtctgatc tactggtgcg ctgcggcgca
     1141 acggtgatgt tttcagaagt cacggaagtg cgtgacgcga tccatctgct gacaccacgc
     1201 gcagtgaacg aagaggtcgg caaacggctg ctggaggaga tggagtggta cgataactat
     1261 ctcaatatgg gaaaaaccga ccgtagcgcc aacccttcgc cgggcaacaa gaaaggcggt
     1321 ctggcaaatg tggtggagaa agcgctcggc tccattgcta aatcgggtaa aagcgcaatt
     1381 gttgaagtgc tgtcgcccgg tcaacgcccg actaaacgcg gattaattta cgccgcgacg
     1441 ccagccagcg attttgtctg tggcacacaa caggtggctt cagggattac cgtgcaagtg
     1501 tttacgaccg gtcgcggtac gccgtacggc ctgatggcga tacccgtcat taaaatggcg
     1561 acccgcaccg agctggcgaa ccgctggttt gatttaatgg atattaacgc gggcactatc
     1621 gctaccggcg aagaaaccat tgaagaagtg ggctggaagt tgttccactt tattctcgac
     1681 gtcgccagcg ggaagaagaa aactttctcg gatcaatggg gactgcataa ccagctggcg
     1741 gtgtttaacc cggcaccagt gacctgattt cttttggaac gtccctcgca aaacatggcc
     1801 ttagtgccat gtttttattg tttaaagtcc acacatccat taataatgca tttgcattac
     1861 cttagttcaa gcttataatt tgagcagaaa acaggctgta aaaggacagt aaataatgcc
     1921 cgctaatgct cgctctaacg ctgtactgac cactgaatca aaggtcacta tacgcggaca
     1981 aacaactatc cccgcgccag tgcgtgaggc cttaaaactg aagccaggcc tggacagcat
     2041 tcattacgaa attctgcctg gtgggcaagt atttatgtgc cgactgggag atgaacagga
     2101 ggatcatact atgaatgcat ttttgcgttt tctggatgca gatatccaga acaacccgca
     2161 aaaaactcgt ccattcgaca ttcaacaagg aaagaaactt gtcgctggca tggacgtcaa
     2221 cattgacgat gagattggcg acgacgaata atggattttc cacaaagggt taatggttgg
     2281 gcgctatatg ctcatccctg ttttcaggaa acctacgatg ctttagttgc cgaagtcgag
     2341 gcattaaagg gaaaagatcc tgaaaattat cagagaaaag ctgccacaaa gttattggcg
     2401 gtggtccata aagtgattga ggagcatatc acggtcaatc catcatcacc ggcattccgt
     2461 catggcaagt cgttaggctc tgggaaaaat aaagactggt cacgggtaaa atttggtgct
     2521 ggtcgctatc gtctcttctt tcgttacagt gaaaaagaga aagtcatcat tctgggatgg
     2581 atgaacgatg aaaacacgct gcgcacctac ggtaaaaaaa cagatgccta taccgtattc
     2641 agtaaaatgt taaaaagagg acatcctcct gccgactggg aaagcctcac ccaagagaca
     2701 gaagaaaacc attgatggtg ttcacaatgc ccgccgaacg cattggtgtt gaccagcggg
     2761 catcctttct cattccccga ccagaatcac ttcaacccca gcctttctca gtccttccag
     2821 actatccgca ggaatgcctt catcaacaat gatcatgtcg atacgttgag tatcaatgat
     2881 cttatgtaaa ctagaacgat tgaacttact ggaatcggtg accacgatga tccgttccgc
     2941 aacttcgcac atccgacggt ttaaacgggc ttcatcttca ttatgcgtgc tgacgccgcg
     3001 ctccagatcg atcgcatcta caccaagaaa cagcatatcg aagtggtaat tttgcagcga
     3061 ttgctcagcc tgatcaccgt aaaaagattg cgactgacgg cgcaaatgcc ccccggtcat
     3121 cagcagctca acgccttccg cttccagcaa cgcattagcc acgttcatac cgttggtcat
     3181 cgcaattacg tcggtgtgct tgcgcatcag acgagcaatc tcaaaagtgg tggtcccgga
     3241 gtcgaggatc acccgatgac caggctgaat caactcaacg gcagctttcg caacgctgcg
     3301 tttcatcgcg gtgttcagcg cgcttttatc ttccactgat ggctcgactg acggcgttgt
     3361 gctatcgcag atcaacgcgc caccataggc acgcacagcg atcccctgct tttccagaaa
     3421 cgccagatcg ttgcggatcg tcacagtaga tacaccatac aacgccgata gatcattaac
     3481 ttgcacactt ccttgctggc gcaggcgttg aatgatctgc tctcgtcgct cgctggtgcc
     3541 tgtcactcgc ttctcacctg aagcgtcggt attactcata gtaagtcctt tcgtaaaact
     3601 ttcgtttcat ttcgttttgc ctattaacgc ctttctatta agcaaatgca agcccacctt
     3661 acccattggc gcaagctact ctcgtttcag tggctttcat tatgtttctt ttgtgaatca
     3721 gatcagaaaa ccattatctt tcgttttatt tttatctcac catgacgcag tatcaactga
     3781 aacaaaacga aagattaatg tcgcagtaat ctgaactgga gaggaaagtg aaacatctga
     3841 cagaaatggt gagacagcac aaagcgggca aaacaaatgg gatttatgcc gtttgttccg
     3901 cacatccgct ggtgctggaa gctgcaatcc gctacgccag tgcaaaccaa acgccgttac
     3961 tgattgaagc aacctccaat caggtggacc agttcggcgg ttataccgga atgacgcccg
     4021 ccgattttcg cggctttgtt tgtcagctcg ccgactcgtt gaatttcccg caggatgcgc
     4081 tgattctggg tggtgaccat ctggggccaa accgctggca aaacctgcct gccgctcagg
     4141 caatggccaa tgccgatgat ttgattaaaa gctacgtggc ggcaggattc aaaaaaattc
     4201 accttgattg cagcatgtcc tgtcaggacg atccgattcc cttaaccgat ggcatcgtgg
     4261 ccgaacgcgc cgcccgtctg gcgaaagtgg cggaagaaac ctgtcttgaa cactttggcg
     4321 aagccgatct ggtgtatgtc attggcaccg aagtaccggt acctggcggc gcgcatgaaa
     4381 ccttaagcga gctggcggtc accacgccgg atgccgcccg cgccacgctg gaagcccatc
     4441 gtcacgcctt tgaaaagcaa ggtctgagtg ccatctggcc acgcatcatt gctctggtgg
     4501 tacaacccgg cgtcgaattc gatcacacca acgttattga ttatcagccc gtcaaagcga
     4561 ccgccttaag ccagatggtc gaaaactacg aaacgctgat tttcgaagcg cactctaccg
     4621 attaccaaac gccgcaatcg ttgcgccagc tagtgattga ccactttgcc atcctgaaag
     4681 ttggcccggc gctgaccttc gccctgcgtg aagctctgtt ctctctggcg gcgattgaag
     4741 aagaactggt accagcaaaa gcctgttctg gtctgcgtca ggtgctggaa aacgtgatgc
     4801 tcgaccgccc ggaatactgg caaagccact accacggtga cggcaacgcg cgtcgtctgg
     4861 cgcgtggtta tagctactcg gatcgcgtgc gctattactg gccggacagc cagattgatg
     4921 acgctttcgc tcatctggtt cgtaatctgg cggattcacc aattccgctg ccgctgatca
     4981 gtcagtatct gccgctgcag tacgtgaaag ttcgctccgg cgagctgcag ccaacgccac
     5041 gggaactcat tatcaaccat attcaggaca tcctggcgca gtaccacaca gcctgtgaag
     5101 gccaataagc aaaacaaaga ggaacacgct atgccaaata ttgttttaag tcggattgat
     5161 gaacgcttga ttcacggtca ggtcggcgtt cagtgggtcg gatttgcggg ggcaaatctg
     5221 gtgctggtag ccaacgatga ggttgccgaa gatccggtac aacaaaacct gatggaaatg
     5281 gtactggcag aagggatcgc cgtacgtttc tggacgctgc aaaaagtcat cgacaacatt
     5341 catcgcgccg ccgatcgaca gaaaatcctg ctagtttgta aaacaccggc cgatttcctg
     5401 acgttggtga aaggtggcgt tccggtgaat cgcattaacg ttggcaatat gcactacgcc
     5461 aatggcaagc aacaaatcgc caaaacggtt tctgtggatg cgggtgatat cgcagcattt
     5521 aacgatctga aagccgctgg ggtggaatgc ttcgttcagg gcgtcccgac agagcctgct
     5581 gtagacctct ttaaattact ttgagggatt catcatggaa atcagtctgt tgcaggcatt
     5641 tgcgttggga attatcgcct ttatcgctgg cctggatatg tttaacggct taacccatat
     5701 gcaccgcccg gtggttctcg gcccgttggt cgggctggta cttggcgatc tgcataccgg
     5761 tattttaacc ggcggtacgc tggaactggt gtggatgggg ctggcccctc tggcgggcgc
     5821 acagccgcct aacgtgatta tcggtactat cgtcggcacg gcgtttgcca ttactactgg
     5881 cgtgaaaccc gatgtcgcgg tcggtgtcgc cgtacctttc gctgtcgcag tacagatggg
     5941 gattaccttc ctgttctcgg tgatgtccgg cgtgatgtcc cgatgcgacc ggatggcgga
     6001 gaacgccgat actcgcggca ttgaacgcgt gaactatctg gcattgctgg cactcggcac
     6061 gttctatttt ctctgcgctt tcctgcctat ctacttcggc gcggaacatg caaaaaccat
     6121 cattgatgtc ctgccgcaac gattaattga tggcctcggc gtcgcgggcg gcatcatgcc
     6181 agcaatcggt tttgccgtgc tgctgaaaat catgatgaaa aacgtctaca tcccctactt
     6241 catcctgggc tttgttgccg cggcctggct caagttaccg gtgctggcta ttgctgccgc
     6301 tgcgctggcg atggcgctga tcgacctgct gcgtaaatct cctgaaccga ctcaacctgc
     6361 ggcacagaaa gaggaatttg aagatggcat ctaatcaaac caccctgccg aacgtcagcg
     6421 aaaatgaaga aacactgctg actggcgtca atgaaaacgt gtatgaagat cagagcattg
     6481 gcgcggagct gacgaaaaaa gatatcaacc gcgtcgcctg gcgttccatg ctgttacagg
     6541 cttctttcaa ctacgaacgt atgcaggctt ccggctggct gtacggcctg ctgccagcac
     6601 tgaaaaagat ccacactaat aaacgcgacc tggcgcgcgc catgaagggg catatgggtt
     6661 tcttcaatac ccatccgttt ctggtgacgt ttgttatcgg cattatcctt gcgatggagc
     6721 gttctaagca ggacgttaac agtattcaga gcaccaaaat tgccgtcggt gcgccgctcg
     6781 gcgggattgg cgatgcgatg ttctggctaa cgctactgcc gatttgtggc gggataggtg
     6841 caagcctcgc gctacaaggc tctattcttg gcgctgtcgt ctttattgtg ctgttcaacg
     6901 tggtgcacct ggggctgcgt tttggtctgg cgcattatgc ttaccgcatg ggcgtggcgg
     6961 cgattccact cattaaagca aataccaaaa aagtcggcca tgcggcatct atcgttggga
     7021 tgacggtaat cggcgcgctg gtggcaacct atgttcgttt aagcaccacg ctggaaatca
     7081 ccgcgggcga cgcagtggtt aagttacagg ctgatgttat cgacaaactg atgccagcct
     7141 tcttaccgct ggtctacacc ctgaccatgt tctggctggt acgccgcggc tggagtccgc
     7201 tgcgcctgat tgcagtgacc gtggttctcg gcatcgtcgg taaattctgc catttccttt
     7261 aaatacaatg aggtattaga tgttaagtat tattttgaca gggcatggcg ggtttgccag
     7321 tggcatggaa aaagcaatga agcaaatcct cggcgagcaa tcgcagttta ttgccatcga
     7381 ttttccggaa acctcaagca ccgcgctgct tacatcgcag cttgaagagg ccatcgccca
     7441 actggattgt gaagacggca ttgtttttct gacggatttg ctgggcggca cgccgtttcg
     7501 cgtagcttca acgctggcga tgcaaaaact gggctgtgaa gtgattaccg gcaccaattt
     7561 gcaactgcta ctggagatgg tgctggagcg cgaagggtta agcggtgaag agtttcgtgt
     7621 gcaggcgctg gagtgtggac atcgtgggct gaccagtctg gtggacgagt taggccgctg
     7681 ccatgaggaa tgtccggtcg aggaaggaat atgacacccg ttttgcgcgc cagaaggctg
     7741 ctgactgaag agggatggct cgatgaccat cagttgcgta ttgctgacgg tgtaatcgca
     7801 gaaatcgaac cgattccagt gggcgtgact gaacgcgatg cggaactgct ctgccccgct
     7861 tacatcgaca cccatgtaca cggtggtgcg ggcgttgatg taatggatga cgcgccggat
     7921 gtgctcgaca agctggcaat gcacaaagca cgcgaaggtg tcggcagttg gttaccgacc
     7981 accgtaaccg cgccgcttaa taccattcat gcggcgctgg aacgtattgc tcaacgttgc
     8041 caacgcggcg gacctggtgc acaaatactg gggagttatc tcgaaggacc gtacttcacg
     8101 ccgcagaata aaggcgcgca tccgccggag ctgtttcgcg agctggaaat tgccgagctg
     8161 gatcaattga ttgccgtttc tcagcacacc ttgcgcgtgg tggcgctggc accggaaaaa
     8221 gaaggggcat tgcaggccat ccgccatctt aaacagcaaa acgtaagggt gatgctgggg
     8281 catagcgcgg cgacctggca acaaacccgc gccgcgtttg atgctggtgc cgacggcctg
     8341 gtgcattgct ataacgggat gacaggttta catcaccgcg aaccgggaat ggttggcgcg
     8401 ggattaacgg acaagcgcgc ctggctggaa ctgatagccg atggtcatca tgtgcatccg
     8461 gcggcgatgt cgctgtgttg ttgctgtgcg aaagagagaa tcgtgctcat caccgacgcg
     8521 atgcaggcag ccgggatgcc ggatggtcgc tatacgttat gtggcgaaga agtgcagatg
     8581 cacggtggcg ttgtccgtac cgcgtccggt ggactggcgg gcagtacgct gtctgtcgat
     8641 gcggcagtgc gcaacatggt cgagttgacg ggcgtaacgc ctgcggaagc catccatatg
     8701 gcgtcgctgc atccggcgcg tatgctgggt gttgatgatg ttctgggatc gcttaaaccg
     8761 ggcaaacgcg ccagcgtcgt tgcgctggat agcgggctgc atgcgcaaca aatctggatt
     8821 cagggtcaat tagcttcgtt ttgatggttt gctcctttat tgggccttca cttcccccgt
     8881 aaggcctttc tttttctttc gttttgatct gtgcagcggt gtcggatgcg acgctaatgc
     8941 gccttatccg acctacggtt ggtgaccgta aggccggata aagtatttac gccgcatcca
     9001 gcaatccctt ttacttccct tgtcttttct ttcaacgatc acagatttcg ttttatttcc
     9061 tttttctcca ttgaactttc agtttctttt ctatagattt taaccaacga aagaaatcac
     9121 caagtgaaat gaaacgaaag gcaaacgaaa gcgacaacgc cgacgtcaag ttcatcagac
     9181 taaggattga gttatgccag aaaattacac ccctgctgct gccgcaaccg gtacatggac
     9241 tgaagaagag atccgccatc agcctcgcgc atggatccgt tcactcacca acatcgacgc
     9301 gctacgttcc gcgctcaata acttccttga accgttactg cgcaaagaga atctgcaaat
     9361 catcctgacc ggagccggaa catcggcatt tatcggtgac atcatcgcgc cgtggctcgc
     9421 cagtcatacc ggtaaaaact tcagcgccgt accgaccacc gatctggtca ctaatccgat
     9481 ggactacctg aatccagctc atccgctgct gttgatctcc ttcggtcgat ccggcaacag
     9541 cccggaaagc gtcgccgccg tggaactggc aaatcaattt gtacccgagt gctatcacct
     9601 gccgatcacc tgcaacgaag cgggcgctct ttaccaaaac gcgatcaaca gcgacaacgc
     9661 gtttgcccta ctgatgcccg cagaaacgca cgaccgcggc tttgcgatga ccagcagcat
     9721 taccaccatg atggccagtt gcctcgcggt tttcgcacct gagacgatca acagccagag
     9781 cttccgcgac gtggcggatc gttgccaggc gatcctgacc tcactgggcg atttcagcga
     9841 aggtgtgttt ggttacgcac cgtggaaacg gatcgtttat ctcggcagcg gtggcttaca
     9901 gggcgcagca cgcgagtcgg cgctgaaagt gctggaactg acagcgggta aactggcggc
     9961 cttctatgat tccccgaccg gattccgtca tggcccgaaa tcgctggtcg ataacgaaac
    10021 gctggtggtg gtatttgtct ccagccaccc ttacacccgt cagtatgatc ttgatctgct
    10081 ggcagaactc cgccgcgata accaggcaat gcgcgtaatc gccatcgccg cggaaagcaa
    10141 tgacgttatt actgccggtc cacatatcat cctgccgccg tcccgtcact ttatcgacgt
    10201 tgagcaggca ttttgcttcc tgatgtacgc ccagacattt gcactgatgc agtcgctgca
    10261 catgggtaat acaccggata ccccatcagc cagtggcacc gttaaccgcg tggtgcaagg
    10321 cgtaatcatt catccgtggc aggcataaga ggatcgcatt atgagcatta tctccaccaa
    10381 atatctgtta caggacgccc aggccaatgg ctacgcggtg cctgccttta acattcacaa
    10441 cgccgagacg atccaggcga tcctcgaagt gtgcagtgaa atgcgatcac cggtgatcct
    10501 cgccgggacg ccggggacct ttaagcatat cgcgctggaa gagatctacg ccctgtgtag
    10561 cgcctattcc acaacctaca acatgccgct ggcactgcat ctcgaccacc acgaatcgct
    10621 ggatgatatt cgccgtaaag tccacgcagg cgtgcgcagc gcgatgatcg acggcagtca
    10681 cttcccgttt gccgagaacg tgaagctggt gaaatcggtt gtcgacttct gccactccca
    10741 ggattgcagc gtggaagcag aactgggccg cctcggcggt gttgaagatg acatgagcgt
    10801 tgacgccgaa agtgcattcc tgaccgatcc acaagaagct aaacgctttg tcgaactgac
    10861 tggcgtcgac agcctggcgg tagcgattgg tacggcgcac ggcttataca gcaaaacgcc
    10921 gaagattgat ttccagcggc tggcggaaat tcgtgaagtg gtggatgttc ctctggtgct
    10981 gcatggtgcc agcgatgtcc cggatgaatt tgtccgtcgc actattgaac ttggcgtcac
    11041 aaaagtgaac gttgccacag aattaaaaat agccttcgct ggcgcggtta aagcctggtt
    11101 tgcggaaaat ccacagggta atgatcctcg ttattatatg cgcgtcggta tggatgcgat
    11161 gaaagaagtt gtcagaaata aaattaatgt ctgtggttca gcgaatcgaa tttcagcata
    11221 atcattagtt ttttatgatt tatcccaatg tacttcccga ttaatatcgg ggagtgcctt
    11281 aatggtaaag gagataacta aaccttaata aatacatcag taaaatatcg caacaataat
    11341 atatttaaaa aaattatatt attcaacttt atggtgagga tcacacaatg agcagtccaa
    11401 atattctctt aacccgtatt gataaccgtc tggttcacgg tcaggttggc gtgacctgga
    11461 catccaccat cggtgcgaat ctgctggtag tcgtggatga tgttgtcgct aacgatgata
    11521 ttcaacagaa attaatgagt attaccgcag aaacctacgg ctttggcatt cgtttcttta
    11581 ctatcgaaaa aaccattaac gtcatcggca aagctgcacc gcatcaaaag atcttcctga
    11641 tttgccgtac gccacaaacg gtacgtaaat tggtagaagg tggtattgac ctgaaagatg
    11701 tcaacgtcgg caatatgcat ttctcggaag ggaaaaagca aatcagcagt aaagtttatg
    11761 tcgatgacca ggatctcacg gacttacgtt ttattaaaca acgtggcgtg aatgttttca
    11821 ttcaggacgt ccctggcgat caaaaagaac aaatccctga ctaaatctaa aatcgcctta
    11881 atattggttt gaggtaataa aaatgcatga aataacccta cttcagggat tatccctggc
    11941 ggcgttagtt ttcgttctgg ggattgattt ttggctggaa gccttatttt tattccgccc
    12001 gataatcgtt tgtaccctaa ctggcgctat tctcggtgat attcagactg gcttaattac
    12061 cggtggtctg acagagttgg ctttcgccgg attaacccct gcaggtggtg ttcagccgcc
    12121 caacccgatt atggcgggtc tgatgaccac cgtcattgca tggtctacgg gcgttgatgc
    12181 caaaacagca attggtcttg gcctgccgtt tagtttgtta atgcagtatg tcattctgtt
    12241 cttctattct gctttctcat tatttatgac caaagccgat aaatgcgcga aagaagcgga
    12301 tacggcagcg ttttcccgac ttaactggac aacgatgctc atcgttgctt cagcgtatgc
    12361 ggtgattgct ttcctctgta cttacctggc acaaggggcg atgcaggcgc tggtgaaagc
    12421 gatgcccgcc tggctgaccc acggctttga agtggctggc ggtattctgc ctgccgttgg
    12481 ttttggcttg ctgctgcgcg tgatgttcaa agcgcaatat atcccttacc tgatcgccgg
    12541 cttcctgttt gtttgctaca tccaggtcag caacctgttg ccggttgccg tactgggcgc
    12601 aggctttgcg gtgtatgagt ttttcaatgc gaaatcccgg cagcaagcgc aaccgcagcc
    12661 cgttgccagt aaaaatgaag aagaggacta cagcaatggg atctgaaatc agtaaaaaag
    12721 atatcacccg tctgggcttt cgttcgtcac tgctacaagc aagctttaac tacgaaagga
    12781 tgcaggcggg cggctttacc tgggcgatgt tgccgctcct gaaaaagatt tataaggacg
    12841 acaaaccagg cctaagcgcg gcgatgaaag ataacctaga atttattaat acccacccga
    12901 atctggtcgg attcctgatg gggttattaa tttcgatgga agagaaaggg gaaaaccgcg
    12961 acaccattaa aggcctcaaa gtggcgctgt ttggcccaat cgccgggatt ggcgatgcga
    13021 ttttctggtt taccttgtta ccaattatgg cggggatttg ctcatcgttt gccagtcagg
    13081 gaaacctgct ggggccgatt ctgttctttg ccgtttacct gcttatcttt ttcttgcgcg
    13141 tcggctggac tcacatcggt tactcggtcg gcgtgaaggc tattgataaa gtgcgagaga
    13201 actcgcagat gattgcccgt tcggcaacca tcctcgggat cacggtaatc ggcgggctga
    13261 tcgcttcgta tgtgcatatt aacgtggtga catcgtttgc catcgacagt acccacagcg
    13321 tcgcgctgca gcaggatttc ttcgataaag tcttcccgaa cattttaccg atggcctaca
    13381 ccctgctgat gtattacttc ctgcgggtga aaaaagcgca tccggtgctg ttaatcggcg
    13441 tgacgtttgt tctttctatt gtttgttctg cattcggcat tttgtaaatg gaacgaggca
    13501 ccgcgtctgg cggtgcctct ttacttaagg aatttcatcc tgtgcaaacc cttcaacaag
    13561 ttgaaaacta tacggcgtta agtgaacgtg ccagcgaata tttattggcc gtgatccgta
    13621 gcaaaccgga tgccgtgatt tgcctggcga ccggagccac gccattactg acgtatcatt
    13681 atctggtaga aaaaatccac cagcagcagg ttgatgtcag ccagctcacc ttcgtgaagc
    13741 tcgacgaatg ggtggatctg ccattaacga tgcctggtac ctgcgaaact ttcctgcaac
    13801 agcatatcgt gcagccgctg gggctacgtg aagaccagct catcagcttt cgctccgaag
    13861 agattaatga gacagagtgc gaacgggtaa cgaacctgat tgcgcgcaaa ggcggtctgg
    13921 atttatgcgt tctcgggttg gggaaaaacg gtcatcttgg gctgaacgaa ccgggagaaa
    13981 gcctgcaacc ggcctgccat atcagtcaac ttgatgccag aacacagcaa catgagatgt
    14041 taaaaaccgc gggccgtcct gtgactcgtg ggatcacctt aggcctgaag gatattctca
    14101 atgcccgcga agttttgtta ctggtgactg gcgaaggaaa gcaggatgcg acagaacgtt
    14161 ttctcacggc taaagtctct accgctatcc cggcttcatt tttatggctg cataactatt
    14221 tcacatgcat gattgatgag atgtgtcgtc gttaactcgc ctgctacagg cgttgcaacg
    14281 ctattcctgc tgcgacgcct gtaatcactc tccctgctgc tccagcgcat acttatacag
    14341 cgcatttttc ttcacgccgt gaatctctgc cgccagcgcc gccgcttttt tcagcggcag
    14401 ttctgcctgt agcagcgcca gcgtgcgcag ggcatcggcg ggtaagtctt cttcctgcgc
    14461 tttatggcct tcgacaatca gcaccatttc gcctttgcga cggttttcat cttctttgac
    14521 ccacgccagc agctcgccaa cgggcgcgcc gtgaatggtt tcccaggttt tggtcagctc
    14581 acgcgccaga accacgtagc gggattcgcc taataccgca acgatatctt ccaggctatc
    14641 taacagacgg tgggtagatt cataaaaaat cagcgtgcgc ggctccgctt caatagcttt
    14701 tagcgcatca cggcggcctt ttgatttggc aggtaaaaag ccttcataac aaaaacgatc
    14761 ggatggtaaa cccgcggcgc ttaacgcagt gatagcagca cacggccctg gtagcggcac
    14821 cacgcgaatc cccgcttcac gacaggtacg caccagatgg tagccaggat cgttaattag
    14881 cggcgttccg gcatcggaaa ccagcgcaat gttttgcccc tcttgcagtt tcgccagcag
    14941 cgtttcggct ttttgttgtt cgttatggtc gtgcagcgca aacaaccggg cattaatccc
    15001 aaaatgttgc agcaataaac cggtgtgacg cgtatcctcg gcggcaatca gatcaacggc
    15061 ctgtaatacc tctaatgcac gctgggtgat atcagccaga ttgccgattg gcgtcggtac
    15121 aatgtaaagc tggccctgag aattatccgc cgattggtgt tgtttcattg tgtcgtccgt
    15181 attgccgatt taatattgag cattgcgtaa aaaaaatatc actggataca ttatggtacc
    15241 ctcaacattt tctcgtttga aagccgcgcg ttgtctgcct gttgttctgg cagccctgat
    15301 tttcgccggt tgtggcaccc atactcccga tcagtccact gcttatatgc agggcacggc
    15361 gcaggctgat tctgcctttt acctgcaaca gatgcagcaa agttctgatg ataccaggat
    15421 caactggcaa ttactcgcca ttcgtgcact ggtgaaagaa ggtaaaaccg gacaggcggt
    15481 ggagttgttt aaccaactac cgcaagaact gaacgattct cagcgtcgcg agaaaacact
    15541 gctggcggca gagattaaac tggcgcagaa agattttgct ggcgcgcaaa acttgctggc
    15601 gaaaatcaca cctgccgatt tagaacaaaa ccagcaagcc cgttactggc aggcaaaaat
    15661 cgatgccagc caggggcgtc cttccattga tttactgcgc gcgttaattg ctcaggaacc
    15721 gctgctcggt gcgaaagaaa agcagcagaa tattgacgcc acatggcagg cgctctcctc
    15781 catgactcag gagcaggcga atacgctggt gatcaacgcc gacgaaaata ttctgcaagg
    15841 ctggctggat ttgcagcgcg tctggtttga taaccgtaac gatcctgaca tgatgaaagc
    15901 tgggatcgcc gactggcaga aacgttatcc gaacaacccg ggcgcgaaaa tgctaccaac
    15961 gcagttggtt aacgtaaaag cgtttaaacc cgcatcgacc aacaaaatcg ccctgctgtt
    16021 gccgctgaat ggccaggcag cggtatttgg tcgcactatt cagcaaggct ttgaagcggc
    16081 gaaaaatatc ggcactcagc cagtggcggc ccaggtagca gccgcacctg ccgcagacgt
    16141 agcagaacaa cctcagccgc aaaccgtgga tggcgttgcc agcccggcac aagcttcggt
    16201 tagcgagctg accggtgaac agcctgcggc ccagccggta cctgtaagcg ccccggcgac
    16261 aagcaccgca gcggtaagcg cacccgcaaa tccatccgca gagctgaaaa tctacgatac
    16321 ctcatcacaa ccacttagcc agatcttaag ccaggttcag caggatggtg cgagtattgt
    16381 ggtcggtccg ttgctgaaaa ataacgttga agagttgctg aagagcaaca ccccgctgaa
    16441 cgtgctggca ctgaaccagc cggagaacat cgaaaaccgc gtcaatattt gttacttcgc
    16501 gctttcaccg gaagacgaag cgcgcgatgc agcgcgtcat attcgtgacc agggtaaaca
    16561 agcgccgctg gtgctgatcc cacgcagttc attgggcgac cgcgtagcca atgcgtttgc
    16621 gcaagagtgg cagaaactgg gcggcggcac cgttctgcaa caaaaatttg gttccaccag
    16681 cgaattacgc gcgggtgtta acggcggttc tggtattgct ttaacgggta gcccgattac
    16741 tcccagagcg acaaccgact ccggcatgac gaccaacaat ccaacgctgc aaaccacgcc
    16801 aaccgatgac cagttcacca ataatggcgg tcgtgtcgat gcggtgtaca ttgtggcaac
    16861 gccgggtgaa atcgctttta tcaaaccgat gatcgccatg cgtaacggta gccagagcgg
    16921 tgcaacgctg tacgccagtt cccgcagcgc acaaggcacc gcaggcccgg atttccgtct
    16981 ggagatggaa ggcttgcagt atagcgaaat cccgatgctg gcaggcggta atctgccgtt
    17041 aatgcagcag gcgctcagcg cggtgaacaa cgattattcg ctggctcgca tgtatgcgat
    17101 gggcgtcgat gcctggtcgt tggcaaatca tttctcacaa atgcgccagg ttcagggttt
    17161 tgaaatcaac ggtaataccg gaagtctgac ggctaacccg gattgcgtga ttaacaggaa
    17221 gttatcatgg ctacagtacc aacaaggtca ggtagtcccc gccagttaac caccaaacag
    17281 accggcgatg tgtgggaagc acaagcgcgt cgctggctgg aaggcaaagg actgcggttt
    17341 atcgccgcta acgtgaacga gcgtggcggc gagatcgatc tgataatgcg tgaaggccgg
    17401 atcaccgttt ttgtcgaggt gcgttaccgc cgctctgcgc tttatggcgg cgcggcagcc
    17461 agtgtgaccc gcagcaaaca acacaaatta ttacagactg cccgcttgtg gctcgcgcgt
    17521 cataatggga gttttgatac tgtggattgc cggttcgatg tggtagcctt caccgggaat
    17581 gaggttgagt ggattaagga tgcctttaat gaccactcat aattaaggtt taaggattag
    17641 cgtgcaagaa agaattaaag cttgcttcac tgaaagcatt caaactcaaa ttgcggcggc
    17701 agaggcgctt ccggatgcca tctcgcgtgc agccatgacg ctggttcagt ctctgctcaa
    17761 tggcaacaaa atcctctgtt gtggtaatgg aacttccgct gccaatgcac agcattttgc
    17821 tgccagcatg atcaaccgtt tcgaaacgga gcggcccagc ttacctgcca ttgcactaaa
    17881 tactgataat gttgtcttaa cggcgattgc caacgatcgc ttacatgatg aagtgtatgc
    17941 aaaacaggtg cgggcgctgg gtcatgcggg agatgtattg ttagccattt ccacccgtgg
    18001 caacagccgc gatattgtaa aagcggtaga agctgccgtt acgcgtgata tgaccattgt
    18061 ggcattaacc ggctatgacg gcggtgaact tgcaggtttg ttagggccac aggatgtgga
    18121 gatccgcatt ccttcgcatc gtagtgctcg cattcaggaa atgcatatgc tgacggtaaa
    18181 ttgcctgtgc gatctgatcg ataacacgct tttccctcac caggatgttt aaggagaata
    18241 catgaaggca ttatcgccaa tcgcagtcct tctttccgcg ctgctgttgc aaggttgtgt
    18301 tgccgctgcc gtagtgggta ccgctgctgt aggtaccaaa gccgcaactg acccacgcag
    18361 tgtcggcacc caggtggacg atggtaccct ggaagtgcgc gtgaacagcg cattgtcgaa
    18421 agacgaacaa attaagaaag aagcgcgcat taatgtcacg gcctatcagg gcaaagtgct
    18481 gctggttggg cagtcaccaa acgctgaact ttcggctcgc gccaaacaga ttgctatggg
    18541 cgtagacggt gccaacgaag tgtataacga gattcgtcag ggccagccga ttggtctggg
    18601 cgaagcgtct aacgatacgt ggatcaccac caaagtacgt tcgcagctgt taaccagcga
    18661 cctggtgaaa tcgtccaacg tgaaagtgac caccgaaaac ggtgaagtgt tcctgatggg
    18721 gctggtgact gaacgtgaag cgaaagcggc ggcagatatt gccagccggg taagcggcgt
    18781 gaagcgggta actacggcgt ttacgtttat taaatagcag cccttgtaat gcctgatgcg
    18841 acgcttgcag cgtcttatca ggcctacaac gatgcaaacc gtaggtcgga taaggcgttt
    18901 acgccgcatc cgacatgttt tccctcaaat caaaccaaca gcgccagccc gccgacaatc
    18961 acaccagaca ctgccaccat cgcccccgtc agccataagg ctttcgccgg gaacgctttg
    19021 cgcagcataa tcagtgacgg caaactcacc gccgggagcg tcatcaacaa tgccagcgcc
    19081 ggagcagttc ccatacctgc cagcatcatc gtttgtacaa tcggaatttc tgctgccgtg
    19141 ggaatgacaa acaagcatcc cgctaccgcc atcgccacca cccacatcag gctgttatcg
    19201 acagcaccat cggcatgggg gaataaccag acgcgagcgg cacccaacac cagtactgca
    19261 aggatgtaaa ccgggatcgt actccagaaa agcgtccata gcgccctgcc ccagcggcta
    19321 aaaaatcctc cctgtgcttc cggtatgtca atttcgaccg gtgcctgcgt ttgcggtgtt
    19381 tcacgcaccc atttttgcac cagcgtcgca atcagcaaca ccatcaccag ccccgccacc
    19441 agacgaatcg ccgcaaaacc ccagccgagg acgaacccca taaacaccag cgtcgccggg
    19501 tttaacaacg gattccccat ccagaatgcc agcgcaccgc ccatcgacac ctgttggcga
    19561 cgcattcccg ccgcgaccgg agccgcacag caggtacaca tcatgcccgg cagcgaaaac
    19621 agcgttccca gcagcgtgcc gcgaaagcgc gattgcccaa gcgtacgcaa taaccagtca
    19681 cgcgggatca gcacctgaat caacgacccg agcagtacgc ccagcaccgc cgctttccat
    19741 accgcgagga aatagatcat cgcgtaatcc aacgccgcct gccacggatt agcatccgcc
    19801 tgcgcaagga tagatttacc gatactgtgg gtttcggcag cagtaaacgc tttgccgtag
    19861 taaggttgcc atttcacata ccagagacca gcaatgacaa cgagaaagaa aagcgcgggt
    19921 ttccaccact gaatgggcgt tgccgcctga gatgaagact gaccagtcat agcattcccc
    19981 aggaataagt tatgataatt gagcgcgtga atattacgct cactatcaat tcttgggaat
    20041 aattattcag ctcttttgcg taattctgaa gagcttaaaa tcgtcacacc ttcatgctca
    20101 ggccgcatcg cctctgccag catgacccgc gccacgtctc tcgcatcaat ggatttccag
    20161 ttacccggta acaaacggaa cagcggcgca aaaagcgttt cgttcatccg ctgtttgcta
    20221 cgatcgccca gtaacatcga cgggcgagca atggtcaatt tcggccagtt ctgggcgatt
    20281 aatgcttctt ccatctcccc tttgacgcgg ttatagaaaa acggcgagtg ggcattagca
    20341 cccatagcac tgaccaccag catatgctgc gcaccgagcc gccgcccggt taatgcggta
    20401 tccaccacca gcgtgtaatc ggcatgaata aacgcctctt tactccccgc ttctcgccgc
    20461 gtggtgccga gacaacaaaa cacaatgtcg atgggatcgg tgacctgcgc cagcgcgtcg
    20521 gtcagttgcg gatcatgggg attaaacaca ccgggcatat cgcccaacgg acgtcgcgtc
    20581 ggcgcggcta tggcgttaac tttcggttca ttaatcaaca tccgcagcag gtgaccaccc
    20641 accagccccg ttgcgcctgt aatcagtacc tggctcatct tcgctccttt acagaattgt
    20701 ctgccttgcg ctccccggct catgcaccag gcttaatagt ctacgaggta attatccccg
    20761 attgtgggaa attcgcctca tccaatgcaa caacgcggag gaagcatgag taagaaaatt
    20821 gccgttttaa tcactgatga atttgaggat tcagaattta cttcacccgc agacgagttc
    20881 cgtaaagccg gacacgaagt gattaccatt gaaaaacaag cgggtaaaac ggtgaaaggc
    20941 aaaaaaggag aagccagcgt ggccatcgat aaatccatcg atgaagtgac gccagcggag
    21001 tttgatgccc tgctgctacc gggcggccat tcaccggatt atctgcgtgg cgacaaccgt
    21061 tttgtcactt tcacccgtga ttttgtgaat agcggcaaac ctgttttcgc catctgtcac
    21121 ggtccgcagc tgctgatcag cgccgatgtg attcgggggc gcaaactgac tgccgttaaa
    21181 ccgatcatta ttgatgttaa aaacgcgggc gcggaatttt acgatcagga agtggtggtc
    21241 gataaagatc agctggttac cagccgtaca ccggacgatc tgccagcgtt taaccgcgag
    21301 gcattacgcc tgctcggtgc ctgagttacg tagccagatc atttttttac caaagcccag
    21361 cgtgttgtcg gtgaatttga tttcatcgag ccggatttcc cacaccgggg ccgacagcat
    21421 tctggcaacc ggaaagcggc gattgtacgc ctggcgcgca aggtcgcttt cctcaccttc
    21481 aagcctgcgg atctcgcctt taaattgcac gccgcgaatt aacgctaccg ttttcggctg
    21541 accgtttacc gttccggcaa ctgccgcctg cggcccactc atctgcgcgt ggcgcgtttt
    21601 ttcttccgtc aaaatgtaga aggcgacttt ctgcgcatca aaaagataaa aggcattagc
    21661 gcaccagagt tccccttcct gctgcacaca ccaggtgaca acatgttgtt ttgccagcca
    21721 acggctgatg gcggtgagtg tttccattgc tgttctcttt tatactgtgg gcctgacctt
    21781 aacatgcgac gcagacgatg acaccctggt ttctttactt gatccgtacc gccgacgata
    21841 agctttatac cgggatcacc acagatgtcg aacgccgcta tcagcagcac caaagcggca
    21901 aaggggcgaa agcactgcgc gggaaaggag aactaacgct ggcgttttcc gcgccagtgg
    21961 gcgatcgttc gctggcacta cgggcggaat atcgcgttaa gcaactgacc aaacggcaaa
    22021 aagagcgtct ggtagcggaa ggcgcagggt ttgcagaact gttgagcagc ctgcaaaccc
    22081 caaagattaa aagcgattga agtgctcgtg atactcaacc agaccagtta caccgttcag
    22141 cgcgtcatcc gctaaacgat gtacctggaa ggcactttca gtgcccggcc agcggcaacg
    22201 cagatcgtga tgtgccgcca gttcaaagcc gaaacggctg tacagcgccg gatcgcccag
    22261 cgtcaccact gcggcatagc cgaactcatt aagcgaatcg agtccttcat agaccagttg
    22321 gcgtgccagc ccctgtccac ggtatttttc atctaccgcc agcggtgcca tgccgaccca
    22381 ttgcaggtct tcgccctgca catcaaccgg actaaatgcc acatagccaa tgacctgacc
    22441 ttcgtcatct gtcgccacca gccccagcgt cagaaagcca tcttcacgca gatcgtgaac
    22501 cagcttcgct tccgcatcac tttcgaatga acgacgcagc agggcatcaa tacccggcgc
    22561 atcaatggga atttctactc gaattagcat ggttcaccta ccgatgtctg tttggtttca
    22621 ggcgcggttt tcattcccgc ctcaacaaaa tccgccagtt gcagcagcat catgcgtaag
    22681 gctttcggca tttgctccag ttcaatggcg tccatcaggt ttttcacata cagccccagc
    22741 tccgtatcgc cttcaattac cagccgacgc tggaagaaga gcgtatccgg atcttgttta
    22801 cgcgccgcaa tcatcagcag atcgctggcg tcggcactaa aactcacatc agcttgcgcg
    22861 ttctggctaa cgaccagttt gccattcacc accgaggtaa accattgcag gtcaatatca
    22921 cgcacatgaa tacttaacca gcggccttca agaaactcca gctcgccatc atccaacgcc
    22981 tggcggaatt gccagcttaa gacctgctca agaacctggc gttttagcgc aaatggcgtc
    23041 agttttaccg gtacactcaa cagagatggc cccaaatgca caatacggga acgcagttta
    23101 tccaacacga gttttactcc ctgtttcaac aatcatccta ttttgccata tcagaaaaat
    23161 aacatagcgg tataaatcaa caattccata tgaaattgct gctaccacca atacaacttt
    23221 aactgcctta aatcaaaaat tgtcgcagca aggttaacta aaatcccagt tcgttaacat
    23281 ttttgcgctt tgatagcgca accttcagga aaaattatgg agctgctctg ccctgccgga
    23341 aatctcccgg cgcttaaggc ggccatcgaa aacggcgcag atgctgttta tatcgggcta
    23401 aaagatgata ccaatgcccg tcacttcgcc ggccttaact ttaccgagaa aaaattgcag
    23461 gaagcggtga gttttgtcca tcaacatcgc cgcaaacttc acatcgcgat taacactttt
    23521 gcgcatccgg acggttacgc ccgctggcag cgcgccgtgg atatggcggc gcagttgggt
    23581 gccgacgcgc tgatcctcgc cgacctcgcc atgctggagt acgccgccga gcgttacccg
    23641 catattgagc gtcatgtgtc agtgcaggct tcggcgacca atgaagaggc gattaacttt
    23701 tatcatcgcc attttgacgt tgcccgcgtg gtgctgccgc gcgtgttgtc gattcatcag
    23761 gtgaaacaac tggcacgggt cacacctgtc ccgctggaag tgtttgcttt cggcagcctg
    23821 tgcattatgt cggaaggccg ttgctatctg tcgtcgtatc tgacgggtga gtcgcccaac
    23881 accgtgggcg cgtgttctcc ggcccgtttc gtgcgctggc agcaaacgcc gcagggactg
    23941 gaatctcgcc tgaacgaagt gctgatcgac cgttatcagg atggcgaaaa cgcaggttat
    24001 ccgacgctgt gtaaagggcg ttatctggtg gacggcgagc gctatcacgc gctggaagaa
    24061 cccaccagtc tcaataccct ggaactgctg ccggagttaa tggcggcgaa tattgcttcg
    24121 gtgaaaattg aaggccgcca gcgtagcccg gcgtatgtca gccaggtggc gaaagtctgg
    24181 cgtcaggcta tcgaccgttg taaggccgat ccgcaaaact tcgtaccgca aagcgcgtgg
    24241 atggagacgc tcgggtcgat gtccgaaggc acgcaaacta cccttggcgc gtatcaccgt
    24301 aaatggcagt gagaaaagca atgaaatatt ccttagggcc ggtgctatgg tactggccaa
    24361 aagagacgct ggaagacttt tatcagcagg ccgccgccag cagcgcagac gtgatttatc
    24421 ttggtgaagc ggtctgtagc aaacgccggg caaccaaagt tggcgactgg ctggagatgg
    24481 caaaatcgct cgccggaagt ggcaagcaga ttgtgctctc cacgctggcg ctggtgcagg
    24541 cgtcctccga acttggcgaa ctgaaacgtt atgttgagaa cggtgagttt ctgattgaag
    24601 ccagcgatct cggcgtggtg aatatgtgcg ctgaacgcaa actgccgttc gtcgccgggc
    24661 acgcgctgaa ctgctacaac gcggtgacac tgaaaatatt gctcaaacag ggcatgatgc
    24721 gctggtgtat gccggtggag ctttcccgcg actggctggt gaatctgctt aatcagtgcg
    24781 atgagctggg cattcgcaac cagtttgaag tggaagtcct gagctacggt catctgccgc
    24841 tggcctactc cgcccgctgc tttaccgcgc gttcggaaga ccgcccgaaa gatgagtgtg
    24901 aaacctgctg cattaagtat ccgaacgggc gcaacgtgct atcgcaggaa aaccaacaag
    24961 tgtttgtact caatggcatt cagaccatga gcggctacgt ttacaacctc ggtaacgagc
    25021 tggcatccat gcaggggctg gtcgatgtgg tccgcctgtc accgcagggt actgacactt
    25081 tcgcgatact cgacgccttc cgcgccaatg aaaatggcgc tgcaccactg ccgttgacgg
    25141 caaacagcga ttgtaacggc tactggcggc ggctggccgg gctggagctg caagcctaag
    25201 taaatagctc actttgttaa caactttaac tactctttaa tgcagtatta aagattaatc
    25261 agtaacaaag tgagctgtta tgactgataa aaccattgcg ttttcgctac tcgatctggc
    25321 ccccattccc gaaggctctt cagcgcggga agcgttctcc cactctctcg acctcgcccg
    25381 tctggctgaa aagcgcggct atcatcgcta ctggctggcg gaacaccaca atatgaccgg
    25441 cattgccagt gctgcaacat cagttttgat tggctatctg gcggcgaata ccactacgct
    25501 gcatctgggg tctggcggcg tgatgttgcc taaccactca ccattggtga tcgccgaaca
    25561 gttcggcacg cttaatacgc tctatccggg gcgaatcgat ttggggctgg gtcgtgcgcc
    25621 gggtagtgac cagcgaacca tgatggcact acgtcgtcat atgagcggtg atattgataa
    25681 tttcccccgc gatgtcgcgg agctggtgga ctggtttgac gcccgcgatc ccaatccgca
    25741 tgtgcgcccg gtaccaggct atggcgagaa aatccccgtg tggttgttag gctccagcct
    25801 ttacagcgcg caactggcgg cgcagcttgg tctgccgttt gcgtttgcct cacacttcgc
    25861 gccggatatg ttgttccagg cgctgcatct ttatcgcagc aacttcaaac cgtcggcacg
    25921 actggaaaaa ccatacgcga tggtgtgcat caatattatc gctgccgaca gcaaccgcga
    25981 cgctgaattt ctgtttacct caatgcagca agcctttgtg aaactacgcc gtggcgaaac
    26041 cggacaacta ccgccaccga ttcaaaatat ggatcagttc tggtcaccgt ctgagcagta
    26101 tggcgtgcag caggcgctga gtatgtcgtt ggtaggtgat aaagcgaaag tgcgtcatgg
    26161 cttgcagtcg atcctgcgcg aaaccgacgc cgatgagatt atggttaacg ggcagatttt
    26221 cgaccaccag gcacgcctgc attcgtttga gctggcgatg gatgtgaagg aagagttgtt
    26281 ggggtagtgt gttttaacgt aggaagcctt atccgagatg gcaacgctgt cctacgtagg
    26341 cctgataagc gaagcgcatc aggcattgtg taggcagcag aaatgtcgga taaggcaccg
    26401 ctgattactg atacaccggc agcaaattaa agctcgataa gatatggacc agtgcgttgc
    26461 caacgccaaa taccagaatc agcataatca tcggcttgcc gccccagacg cggaatttcg
    26521 ggctaccaaa gcgtttacgc gatgcgcggg ccaacagcgc cggaacaatt gccgcccaga
    26581 tggtagccgc taagccagca taaccaatgg cgtacaggaa tccgttagga aacagcaggc
    26641 cacccacaac aggcggggca aaggtcagca acgctgtttt caaacggccc atagccgagt
    26701 cgtcgaaacc aaacagatct gccagatagt caaacaaacc cagcgttacg ccaaggaacg
    26761 aactcgctac cgcaaagttt gagaacacga ccagcagaag atccaggcta cggctgttca
    26821 gcacgccgct taatgcctgt accagcacat caatattacc gcccttctcg gcgatgccga
    26881 taaactccgg acgagggatg ttgcccatcg tcgccagcaa ccagatggta tacagcgcca
    26941 gcgccatcag cgtaccgtac accaggcatt tcacgatggt tttcggatct ttgccgtaat
    27001 acttcatcag gctcggcacg ttaccgtgat aaccaaacga tgccagacag aatggcagtg
    27061 tcatcagcag atacggcgca taagacgcat tgctttcggc gacgttgaac aaggtcgttg
    27121 gctgtacatg ccccagcagg ctgccgaagg tgaggaagaa agtaatgact ttcgccccca
    27181 gcacaattgc tgtcatccgg ctgacagctt tcgtactcaa ccacaccacg aacgctacca
    27241 gcagtgcaaa accaaatcca gctgcccgtg cagggacgtt cagcgacatc tccgcgaagg
    27301 tgtgatgcag aatcgaaccg ctggcagaaa tataggcgta agtcaggata tagagcacaa
    27361 aggcaatgga aatgccgttg actacgttcc agcctttgcc cagcaaatct ttggtgatgg
    27421 tgtcaaagct agaaccgatt cgataattca ggttagcttc cagaatcatc aagccggaat
    27481 gcagcataca gaaccaggta aagatcagcg ccgccattga ccagaagaac cacgccccgg
    27541 acatgaccac aggcagagaa aacatccctg cgccaataat ggtgccgccg ataatcacca
    27601 cgccgccaag cagcgacggt gacgtttggg tggtggttag tgttgccatg agggcttctc
    27661 tccagtgaaa aatagtgcga ctgcgttgtt atgcattgca ctgtaccagt acacgagtac
    27721 aaaagaca
//
LOCUS       DAZFLD010000043        27605 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.043, whole genome shotgun sequence.
ACCESSION   DAZFLD010000043 DAZFLD010000000
VERSION     DAZFLD010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27605
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.043"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            71..802
                     /gene="artJ"
                     /locus_tag="ACHKBL_001545"
     CDS             71..802
                     /gene="artJ"
                     /locus_tag="ACHKBL_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein ArtJ"
                     /protein_id="HFO5818430.1"
                     /translation="MKKLVLAALLASFTFGASAAEKINFGVSATYPPFESIGANNEIV
                     GFDIDLAKALCKQMQAECTFTNHAFDSLIPSLKFRKYDAVISGMDITPERSKQVSFTT
                     PYYENSAVVIAKKDTYKTFADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAF
                     IDLKNGRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLE
                     KLNNALAAIKADGTYQKINDQWFPQ"
     gene            complement(831..1958)
                     /gene="rlmC"
                     /locus_tag="ACHKBL_001546"
     CDS             complement(831..1958)
                     /gene="rlmC"
                     /locus_tag="ACHKBL_001546"
                     /EC_number="2.1.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HFO5818431.1"
                     /translation="MQCALYDAGRCRSCQWITQPIPEQLSAKTADLKNLLADFPVEEW
                     CAPVSGPEQGFRNKAKMVVSGSVEKPLLGMLHRDGTPEDLCDCPLYPASFAPVFAALK
                     PFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSDTKLAQLRKALPWLQE
                     QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALAERFNDVPLWIRPQSFFQTNPAVA
                     SQLYATARDWVRQLSVKHMWDLFCGVGGFGLHCAMPDMQLTGIEIAPEAIACAKQSAA
                     ELGLTRLQFQALDSTQFATAQGEVPELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSC
                     NAQTMAKDIRELPGYCIERVQLFDMFPHTAHYEVLTLLVKQ"
     gene            complement(1999..2487)
                     /locus_tag="ACHKBL_001547"
     CDS             complement(1999..2487)
                     /locus_tag="ACHKBL_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjO family protein"
                     /protein_id="HFO5818432.1"
                     /translation="MEDETLGFFKKTSSSHARLNVPALVQVAALAIIMIRGLDVLMIF
                     NTLGVRGIGEFIHRSVQTWSLTLVFLSSLVLVFIEIWCAFSLVKGRRWARWLYLLTQI
                     TAASYLWAASLGYGYPELFSIPGESKREIFHSLMLQKLPDMLILMLLFVPSTSRRFFQ
                     LQ"
     gene            complement(2547..3392)
                     /gene="potI"
                     /locus_tag="ACHKBL_001548"
     CDS             complement(2547..3392)
                     /gene="potI"
                     /locus_tag="ACHKBL_001548"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
                     /protein_id="HFO5818433.1"
                     /translation="MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVW
                     AGWSTRWYGELLRDNAMMSAVGLSLTIAACAATAAAILGTIAAVVLVRFGRFRGSNGF
                     AFMITAPLVMPDVITGLSLLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVIS
                     SRLRELDRSIEEAAMDLGATPLKVFFVITLPMIMPAIISGWLLAFTLSLDDLVIASFV
                     SGPGATTLPMLVFSSVRMGVNPEINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQ
                     RARRG"
     gene            complement(3389..4342)
                     /gene="potH"
                     /locus_tag="ACHKBL_001549"
     CDS             complement(3389..4342)
                     /gene="potH"
                     /locus_tag="ACHKBL_001549"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
                     /protein_id="HFO5818434.1"
                     /translation="MSTLEPAAQPKPPGGFKLWLSQLQMKHGRKLVIALPYIWLILLF
                     LLPFLIVFKISLAEMARAIPPYTELMEWADGQLSITLNLGNFLQLTDDPLYFDAYLQS
                     LQVAAISTFCCLLIGYPLAWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGILKN
                     NGVLNNFLLWLGVIDQPLTILHTNLAVYIGIVYAYVPFMVLPIYTALIRIDYSLVEAA
                     LDLGARPLKTFFTVIVPLTKGGIIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQ
                     EFFNNRDWPVASAVAIIMLLLLIVPIMWFHKHQQKSVGEHG"
     gene            complement(4352..5485)
                     /gene="potG"
                     /locus_tag="ACHKBL_001550"
     CDS             complement(4352..5485)
                     /gene="potG"
                     /locus_tag="ACHKBL_001550"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015417 - ABC-type polyamine transporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter ATP-binding subunit
                     PotG"
                     /protein_id="HFO5818435.1"
                     /translation="MNDAIPRPQAKTRKVLTPLLEIRNLTKSYDGQHAVDDVSLTIYK
                     GEIFALLGASGCGKSTLLRMLAGFEQPSAGQIMLDGVDLSQVPPYLRPINMMFQSYAL
                     FPHMTVEQNIAFGLKQDKLPKAEIASRVNEMLGLVHMQEFAKRKPHQLSGGQRQRVAL
                     ARSLAKRPKLLLLDEPMGALDKKLRDRMQLEVVDILERVGVTCVMVTHDQEEAMTMAG
                     RIAIMNRGKFVQIGEPEEIYEHPTTRYSAEFIGSVNVFEGVLKERQEDGLVLYSPGLV
                     HPLKVDVDASVVDNVPVHVALRPEKIMLCEEPPADGFNFAVGEVMHIAYLGDLSVYHV
                     RLKSGQMISAQLQNAHRYRKGLPTWGDEVRLCWEVDSCVVLTV"
     gene            complement(5580..6692)
                     /gene="potF"
                     /locus_tag="ACHKBL_001551"
     CDS             complement(5580..6692)
                     /gene="potF"
                     /locus_tag="ACHKBL_001551"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotF"
                     /protein_id="HFO5818436.1"
                     /translation="MTALNKKWLSGLVTGALMAVSVGTLAAEQKTLHIYNWSDYIAPD
                     TVANFEKETGIKVVYDVFDSNEVLEGKLMAGSTGFDLVVPSASFLERQLTAGVFQPLD
                     KSKLPEWKNLDPELLKLVAKHDPDNKFAMPYMWATTGIGYNVDKVKAVLGENAPVDSW
                     DLILKPENLEKLKSCGVSFLDAPEEVFATVLNYLGKDPNSTKADDYTGPATDLLLKLR
                     PNIRYFHSSQYINDLANGDICVAIGWAGDVWQASNRAKEAKNGVNVSFSIPKEGAMAF
                     FDVFAMPADAKNKDEAYQFLNYLLRPDVVAHISDHVFYANANKAATPLVSAEVRDNPG
                     IYPPADVRAKLFTLKVQDPKIDRVRTRAWTKVKSGK"
     gene            complement(7043..7519)
                     /locus_tag="ACHKBL_001552"
     CDS             complement(7043..7519)
                     /locus_tag="ACHKBL_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HFO5818437.1"
                     /translation="MTSLVVPGLDTLRQWLDDLGMSFFECDNCQALHLPHMQNFDGVF
                     DAKIDLIDNTILFSAMAEVRPSAVLPLAADLSAINASSLTVKAFLDMQDDNLPKLVVC
                     QSLSVMQGVTYEQFAWFVRQSEEQISMVILEANAHQLLLPTDDEGQNNVTENYFLH"
     gene            complement(7607..8509)
                     /gene="rimK"
                     /locus_tag="ACHKBL_001553"
     CDS             complement(7607..8509)
                     /gene="rimK"
                     /locus_tag="ACHKBL_001553"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HFO5818438.1"
                     /translation="MKIAILSRDGTLYSCKRLREAAIQRGHLVEILDPLSCYMNINPA
                     ASSIHYKGRKLPHFDAVIPRIGTAITFYGTAALRQFEMLGSYPLNESVAIARARDKLR
                     SMQLLARQGIDLPVTGIAHSPDDTSDLIDMVGGAPLVVKLVEGTQGIGVVLAETRQAA
                     ESVIDAFRGLNAHILVQEYIKEAQGCDIRCLVVGDEVVAAIERRAKEGDFRSNLHRGG
                     AASVASITPQEREIAIKAARTMALDVAGVDILRANRGPLVMEVNASPGLEGIEKTTGI
                     DIAGKMIRWIERHATTEYCLKTGG"
     gene            complement(8570..9292)
                     /gene="nfsA"
                     /locus_tag="ACHKBL_001554"
     CDS             complement(8570..9292)
                     /gene="nfsA"
                     /locus_tag="ACHKBL_001554"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase NfsA"
                     /protein_id="HFO5818439.1"
                     /translation="MTPTIELICGHRSIRHFTDEPISEAQREAIINSARATSSSSFLQ
                     CSSIIRITDKALRDELVTLTGGQKHVAQAAEFWVFCADFNRHLQICPDAQLGLAEQLL
                     LGVVDTAMMAQNALTAAESLGLGGVYIGGLRNNIEAVTELLKLPQHVLPLFGLCLGWP
                     ADNPDLKPRLPASILVHENNYQPLDKDALAQYDEQLAEYYLTRGSNNRRDTWSDHIRR
                     TIIKESRPFILDYLHKQGWATR"
     gene            complement(9276..9563)
                     /locus_tag="ACHKBL_001555"
     CDS             complement(9276..9563)
                     /locus_tag="ACHKBL_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1418 family protein"
                     /protein_id="HFO5818440.1"
                     /translation="MRAIGKLPKSVLILEFIGMMLLAVALLSVSGSLSLPEPFSRPEV
                     QILMIFLGVLLMLPAAVVVILQVAKRLAPQLMNRPPQYSRSEREKDNDANH"
     gene            9723..9980
                     /gene="grxA"
                     /locus_tag="ACHKBL_001556"
     CDS             9723..9980
                     /gene="grxA"
                     /locus_tag="ACHKBL_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 1"
                     /protein_id="HFO5818441.1"
                     /translation="MQTVIFGRPGCPYCVRAKDLAEKLSNERDDFQYQYVDIRAEGIT
                     KEDLQQKAGKPVETVPQIFVDQQHIGGYTDFAAWVKENLDA"
     gene            complement(10010..10387)
                     /locus_tag="ACHKBL_001557"
     CDS             complement(10010..10387)
                     /locus_tag="ACHKBL_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YbjM"
                     /protein_id="HFO5818442.1"
                     /translation="MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLF
                     FILPGAVASFFSHRREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFW
                     CALGALCFLFISSLFKPQHRKNQ"
     gene            10657..12342
                     /locus_tag="ACHKBL_001558"
     CDS             10657..12342
                     /locus_tag="ACHKBL_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine antiporter"
                     /protein_id="HFO5818443.1"
                     /translation="MNINVAELLNGNYILLLFVVLALGLCLGKLRLGSIQLGNSIGVL
                     VVSLLLGQQHFSINTDALNLGFMLFIFCVGVEAGPNFFSIFFRDGKNYLMLALVMVGS
                     ALVIALGLGKLFGWDIGLTAGMLAGSMTSTPVLVGAGDTLRHSGMESRQLSLALDNLS
                     LGYALTYLIGLVSLIVGARYLPKLQHQDLQTSAQQIARERGLDTDANRKVYLPVIRAY
                     RVGPELVAWTDGKNLRELGIYRQTGCYIERIRRNGILANPDGDAVLQMGDEIALVGYP
                     DAHARLDPSFRNGKEVFDRDLLDMRIVTEEVVVKNHNAVGKRLAQLKLTDHGCFLNRV
                     IRSQIEMPIDDNVVLNKGDVLQVSGDARRVKTIADRIGFISIHSQVTDLLAFCAFFVI
                     GLMIGMITFQFSTFSFGMGNAAGLLFAGIMLGFMRANHPTFGYIPQGALSMVKEFGLM
                     VFMAGVGLSAGSGINNGLGAIGGQMLIAGLIVSLVPVVICFLFGAYVLRMNRALLFGA
                     MMGARTCAPAMEIISDTARSNIPALGYAGTYAIANVLLTLAGTIIVMVWPGLG"
     gene            complement(12518..13054)
                     /gene="rcdA"
                     /locus_tag="ACHKBL_001559"
     CDS             complement(12518..13054)
                     /gene="rcdA"
                     /locus_tag="ACHKBL_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RcdA"
                     /protein_id="HFO5818444.1"
                     /translation="MRRANDPQRREKIIQATLEAVKLYGIHAVTHRKIATLAGVPLGS
                     MTYYFSGIDELLLEAFSSFTEIMSRQYQAFFSDVSDAPGACQAITDMIYSSQVATPDN
                     MELMYQLYALASRKPLLKTVMQNWMQRSQQTLEQWFEPGTARALDAFIEGMTLHFVTD
                     RKPLSREEILRMVERVAG"
     gene            13138..14346
                     /locus_tag="ACHKBL_001560"
     CDS             13138..14346
                     /locus_tag="ACHKBL_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO5818445.1"
                     /translation="MTVNSSRNALKRRTWALFMFFFLPGLLMASWATRTPAIRDILSV
                     SIAEMGGVLFGLSIGSMSGILCSAWLVKRFGTRNVILVTMSCALIGMMILSLALWLTS
                     PLLFAVGLGVFGASFGSAEVAINVEGAAVEREMNKTVLPMMHGFYSLGTLAGAGVGMA
                     LTAFGVPATVHILLAALVGIAPIYIAIQAIPDGTGKNAADGTQHGEKGVPFYRDIQLL
                     LIGVVVLAMAFAEGSANDWLPLLMVDGHGFSPTSGSLIYAGFTLGMTVGRFTGGWFID
                     RYSRVAVVRASALMGALGIGLIIFVDSAWVAGVSVVLWGLGASLGFPLTISAASDTGP
                     DAPTRVSVVATTGYLAFLVGPPLLGYLGEHYGLRSAMLVVLALVILAAIVAKAVAKPD
                     TKTQTAMENS"
     gene            14346..15161
                     /gene="ybjI"
                     /locus_tag="ACHKBL_001561"
     CDS             14346..15161
                     /gene="ybjI"
                     /locus_tag="ACHKBL_001561"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase"
                     /protein_id="HFO5818446.1"
                     /translation="MSIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVAS
                     GNQYYQLISFFPEIANEIAFVAENGGWVVSEGKDVFNGELSKDAFSTVVEHLLTRPEV
                     EIIACGKNSAYTLKKYDDAMKTVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQ
                     VQKALHEAIGDIMVPVHTGNGSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGDGGN
                     DIEMLRQAGFSFAMENAGSAVVAAAKYRAGSNNREGVLDVIDKVLKHEAPFNQ"
     gene            15246..15530
                     /gene="ybjH"
                     /locus_tag="ACHKBL_001562"
     CDS             15246..15530
                     /gene="ybjH"
                     /locus_tag="ACHKBL_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbjH"
                     /protein_id="HFO5818447.1"
                     /translation="MIMKNCLLLGALLMGFTGVAMAQSVTVDVPSGYKVVVVPDSVSV
                     PQAVSVATVPQTVYVAPAPAPAYRPHPYVRHLASVGEGMVIEHQIDDHHH"
     gene            complement(15571..16803)
                     /gene="mdfA"
                     /locus_tag="ACHKBL_001563"
     CDS             complement(15571..16803)
                     /gene="mdfA"
                     /locus_tag="ACHKBL_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdfA"
                     /protein_id="HFO5818448.1"
                     /translation="MQNKLASGARLGRQALLFPLCLVLYEFSTYIGNDMIQPGMLAVV
                     EQYQAGIDWVPTSMTAYLAGGMFLQWLLGPLSDRIGRRPVMLAGVVWFIVTCLAILLA
                     QNIEQFTLLRFLQGISLCFIGAVGYAAIQESFEEAVCIKITALMANVALIAPLLGPLV
                     GAAWIHVLPWEGMFVLFAALAAISFFGLQRAMPETATRIGEKLSLKELGRDYKLVLKN
                     GRFVAGALALGFVSLPLLAWIAQSPIIIITGEQLSSYEYGLLQVPIFGALIAGNLLLA
                     RLTSRRTVRSLIIMGGWPIMIGLLVAAAATVISSHAYLWMTAGLSIYAFGIGLANAGL
                     VRLTLFASDMSKGTVSAAMGMLQMLIFTVGIEISKHVWLNGGNGLFNLFNLVNGILWL
                     LLMVIFLKDKQMGNSHEG"
     gene            17088..17684
                     /gene="ybjG"
                     /locus_tag="ACHKBL_001564"
     CDS             17088..17684
                     /gene="ybjG"
                     /locus_tag="ACHKBL_001564"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFO5818449.1"
                     /translation="MLENLNLSLFSLINATPDSAPWMISLAIFIAKDLITVVPLLAAV
                     LWLWGLTAQRQLVIKIAIALAVSLFVSWSMGHLFPHDRPFVENIGYNFLHHAADDSFP
                     SDHGTVIFTFALAFLCWHRLWSGSLLMVLAVVIAWSRVYLGVHWPLDMLGGLLAGMIG
                     CLSAQIIWQAMGHKLYQRLQSWYRFCFALPIRKGWVRD"
     gene            17742..18500
                     /gene="deoR"
                     /locus_tag="ACHKBL_001565"
     CDS             17742..18500
                     /gene="deoR"
                     /locus_tag="ACHKBL_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor DeoR"
                     /protein_id="HFO5818450.1"
                     /translation="METRREERIGQLLQELKRSDKLHLKDAAALLGVSEMTIRRDLNN
                     HSAPVVLLGGYIVLEPRSASHYLLSDQKSRLVEEKRRAAKLAATLVEPDQTLFFDCGT
                     TTPWIIEAIDNEIPFTAVCYSLNTFLALKEKPHCRAFLCGGEFHASNAIFKPIDFQQT
                     LNNFCPDIAFYSAAGVHVSKGATCFNLEELPVKHWAMSMAQKHVLVVDHSKFGKVRPA
                     RMGDLKRFDIVVSDCCPEDEYVKYAQTQRIKLMY"
     gene            complement(18547..19749)
                     /gene="dacC"
                     /locus_tag="ACHKBL_001566"
     CDS             complement(18547..19749)
                     /gene="dacC"
                     /locus_tag="ACHKBL_001566"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HFO5818451.1"
                     /translation="MTQYSSLLRGLAAGSAFLFLFAPTAFAAEQTVEAPSVDARAWIL
                     MDYASGKVLAEGNADEKLDPASLTKIMTSYVVGQALKADKIKLTDMVTVGKDAWATGN
                     PALRGSSVMFLKPGDQVSVADLNKGVIIQSGNDACIALADYVAGSQESFIGLMNGYAK
                     KLGLTNTTFQTVHGLDAPGQFSTARDMALLGKALIHDVPEEYAIHKEKEFTFNKIRQP
                     NRNRLLWSSNLNVDGMKTGTTAGAGYNLVASATQGDMRLISVVLGAKTDRIRFNESEK
                     LLTWGFRFFETVTPIKPDATFVTQRVWFGDKSEVNLGAGEAGSVTIPRGQLKNLKASY
                     TLTEPQLTAPLKKGQVVGTIDFQLNGKSIEQRPLIVMENVEEGGFFGRMWDFVMMKFH
                     QWFGSWFS"
     gene            19996..20622
                     /gene="gstB"
                     /locus_tag="ACHKBL_001567"
     CDS             19996..20622
                     /gene="gstB"
                     /locus_tag="ACHKBL_001567"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase GstB"
                     /protein_id="HFO5818452.1"
                     /translation="MITLWGRNNSTNVKKVLLTLEELELPYEQILAGREFGINHDADF
                     LAMNPNGLVPLLRDDESDLILWESNAIVRYLAAQYGQKRLWIDSPARRAEAEKWMDWA
                     NQTLSNAHRGILMGLVRTPPEERDQAAIDASCKECDALFALLDAELAKVKWFSGDEFG
                     VGDIAIAPFIYNLFNVGLTWTPRPNLQRWYQQLTERPAVRKVVMIPVS"
     gene            complement(20619..21734)
                     /gene="yliI"
                     /locus_tag="ACHKBL_001568"
     CDS             complement(20619..21734)
                     /gene="yliI"
                     /locus_tag="ACHKBL_001568"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose sugar dehydrogenase YliI"
                     /protein_id="HFO5818453.1"
                     /translation="MHRQSFFLVPLICLSSALWAAPATVNVEVLQDKLDHPWALAFLP
                     DNHGMLITLRGGELRHWQAGKGLSAPLSGVPDVWAHGQGGLLDVVLAPDFAQSRRIWL
                     SYSEVGDDGKAGTAVGYGRLSDDLSKVTDFRTVFRQMPKLSTGNHFGGRMVFDGKGYL
                     FIALGENNQRPTAQDLDKLQGKLVRLTDLGEIPDDNPFIKESDARAEIWSYGIRNPQG
                     MAMNPWSNALWLNEHGPRGGDEINIPQKGKNYGWPLATWGINYSGFKIPEAKGEIVAG
                     TEQPVFYWKDSPAVSGMAFYNSDKFPQWQQKLFIGALKDKDVIVMSVNGDKVTEDGRI
                     LTDRGQRIRDVRTGPDGYLYVLTDESSGELLKVSPRN"
     gene            complement(21845..22228)
                     /gene="bssR"
                     /locus_tag="ACHKBL_001569"
     CDS             complement(21845..22228)
                     /gene="bssR"
                     /locus_tag="ACHKBL_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssR"
                     /protein_id="HFO5818454.1"
                     /translation="MFVDRQRIDLLNRLIDARVDLAAYVQLRKAKGYMSVSESNHLRD
                     NFFKLNRELHDKSLRLNLHLDQEEWSALHHAEEALATAAVCLMSGHHDCPTVITVNAD
                     KLENCLMSLTLSIQSLQKHAMLEKA"
     gene            22441..23766
                     /gene="rimO"
                     /locus_tag="ACHKBL_001570"
     CDS             22441..23766
                     /gene="rimO"
                     /locus_tag="ACHKBL_001570"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HFO5818455.1"
                     /translation="MSKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDD
                     ADMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITG
                     PHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
                     SMRGDLVSRPIGEVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTS
                     MVSLCEQLSKLGIWTRLHYVYPYPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMK
                     RPGSVDRQLARIKQWREICPELTLRSTFIVGFPGETEEDFQMLLDFLKEARLDRVGCF
                     KYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIIDEVDE
                     EGAIGRSMADAPEIDGAVYLNGETNVKPGDILRVKVEHADEYDLWGSRV"
     gene            23992..24378
                     /locus_tag="ACHKBL_001571"
     CDS             23992..24378
                     /locus_tag="ACHKBL_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO5818456.1"
                     /translation="MGVYKARRFSQSTKKLGIHDKVLMAAAEEVIQGIWEADLGSGVI
                     KKRLPLQQGKSGGARTIIFFKSANHVFFYDGWSKSGLSSKGTKEIEDDELAAYKKMAN
                     AFLAFSNKQIEDLIETGFLIEVKNER"
     gene            24368..24697
                     /locus_tag="ACHKBL_001572"
     CDS             24368..24697
                     /locus_tag="ACHKBL_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO5818457.1"
                     /translation="MSDKLQMMLEDAKELNALGLMSDSEVDAIAVMVKAREVSARIAK
                     VKSMTGEEIKAIRTRYGMSQSVLAHTMGMSKESVSKWERNEIKPSGPALRILNTLAIK
                     GPEVFAK"
     gene            complement(24767..26095)
                     /locus_tag="ACHKBL_001573"
     CDS             complement(24767..26095)
                     /locus_tag="ACHKBL_001573"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFO5818458.1"
                     /translation="MSRINKFVLTVSLLIFIIISAVACGIYTQMVKERVYSLKQSVID
                     TAFAVANIAEYRRSVAIDLINTLNPTEEQLLVGLRTAYADSVSPSYLYDVGPYLISSD
                     ECIQVKEFEKNYCADIMQVVKYRHVKNTGFISFDGKTFVYYLYPVTHNRSLIFLLGLE
                     RFSLLSKSLAMDSENLMFSLFKNGKSVTGDEYNAKNAIFTVSEAMEHFAYLPTGLYVF
                     AYKKDVYFQVCTLIIFFAALVAVISGASCLYLVRRVINRGIVEKEAIINNHFERVLDG
                     GLFFSAADVKKLYSMYNSAFLDDLTKAMGRKSFDEDLKALPEKGGYLCLFDVDKFKNI
                     NDTFGHLLGDEVLMKVVKILKSQLPVDKGKVYRFGGDEFAVIYTGGTLEELLSILKEI
                     VHFQVGSINLSTSIGVAHSNECTTVERLKMLADERLYKSKKNGRAQISWQ"
     gene            complement(26103..27605)
                     /locus_tag="ACHKBL_001574"
                     /pseudo
     CDS             complement(26103..27605)
                     /locus_tag="ACHKBL_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415354.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic diguanylate phosphodiesterase"
BASE COUNT         7107 a         7104 c         6614 g         6780 t
ORIGIN      
        1 ttgatggcat tgttatccca tgccgcagac acggccaaaa atcataagat tgacagacgg
       61 gagttccatc atgaaaaagt tagttcttgc cgcattactt gcttccttta ctttcggtgc
      121 ttctgccgca gagaaaatca attttggcgt ttcagccacc tatccaccct ttgaatctat
      181 aggtgctaat aatgagattg tcggctttga tatcgacctg gcaaaagcct tgtgcaaaca
      241 aatgcaggca gaatgtactt ttactaatca cgcgttcgac agcctgatcc cgtcactgaa
      301 attcagaaaa tatgacgccg taatctccgg tatggatatc actccagagc gtagcaaaca
      361 ggtatcgttt accacgccct actatgaaaa ctcagccgtc gtgattgcca aaaaagatac
      421 ctacaaaacg tttgccgatc tgaaaggcaa acgtattggg atggaaaacg gtactacgca
      481 ccagaaatat attcaggatc agcacccgga agtgaaaact gtctcctatg acagttatca
      541 gaatgccttt atcgatctga aaaatggccg tattgatggg gtatttggtg acacagcggt
      601 ggttaatgaa tggctgaaaa ccaacccgca actgggcgtt gcgactgaaa aagtgaccga
      661 tccgcaatac ttcggcaccg gcctgggtat cgctgtacgt ccggataaca aagctctgct
      721 ggaaaaactg aataacgcgc tggcagcaat taaagctgac ggtacatatc agaaaatcaa
      781 tgaccagtgg ttcccgcagt aagattcaga aagccgcacc tgcggctttt ttattgctta
      841 accagcagtg tcagcacttc gtaatgcgca gtgtgcggga acatatcaaa aagctgtacc
      901 cgttcgatgc agtaacctgg cagttcgcgg atatctttcg ccatggtttg ggcgttacag
      961 ctggagtaga taataaaacg cggtgccatc gttgagagat aatcacacag cggtttacca
     1021 atgccgcggc gcggcgggtt aaccagcacc agctccggca cctccccctg agcagtggca
     1081 aactgagtgg agtccagcgc ctgaaattgc aaacgcgtta agcccagttc agcggctgac
     1141 tgctttgcac aggcaatggc ctctggcgca atttcgatcc cggttaactg catgtcaggc
     1201 atcgcgcagt gtaaaccaaa gccccccaca ccgcagaaca gatcccacat atgtttaacc
     1261 gacagctgtc gtacccagtc gcgcgcggtg gcgtacaact ggctggcgac cgccggatta
     1321 gtctggaaga aactttgcgg acggatccac agcggtacgt cattaaaacg ctctgccagc
     1381 gcctgttgtt cagtcaggta aatctccgtc tccccttcca taatcgccat atgtaccggc
     1441 tgaatattga cggtaataac tttcagctgc ggtagttgtt cctgtaacca cggcagcgcc
     1501 ttacgcagtt gcgccagctt ggtatcagaa cgcagtacaa agcgcagcat catgccgcca
     1561 tcgctctggc tttcagtcag cagaatgtat ttcagttcgc cacgcttacg cgccacgttg
     1621 tagggcgtta accccgctcg ggcgataaac ggttttagcg ccgcaaaaac gggcgcaaat
     1681 gaggcaggat aaagcggaca gtcacaaagg tcttccggcg tgccgtcgcg atgcagcata
     1741 ccgagcagtg gtttttcaac gctaccactc accaccattt tggctttatt acgaaaccct
     1801 tgttccgggc ctgacaccgg cgcgcaccat tcctcaaccg gaaagtcggc gagcagattt
     1861 ttaagatcgg cggttttagc ggagagttgc tctggaatcg gctgcgttat ccactgacag
     1921 gaacgacagc gacccgcgtc gtaaagtgcg cactgcatac attgaccttc acatcatcag
     1981 gggcgacgat tatacacatt attgcaactg gaagaaccgc cgactggtcg agggaacgaa
     2041 cagcagcatc aggatgagca tatccggcag cttctgcagc atcaggctat ggaagatttc
     2101 acgttttgat tcaccgggaa tgctgaacag ctccggataa ccatacccca gcgaagctgc
     2161 ccacaagtaa cttgcggccg tgatttgtgt caacagatac agccagcgcg cccagcgacg
     2221 ccctttcacc agtgaaaacg cacaccagat ctcaatgaaa accagcacca gactgctcaa
     2281 aaagaccagc gttaaactcc aggtttgtac gctgcgatga atgaactcgc caataccgcg
     2341 cacgcccagc gtattgaaaa tcatcagcac gtcgaggcca cggatcataa taatggcgag
     2401 cgccgccacc tgcaccagcg caggcacatt caggcgagca tgagatgaag atgttttctt
     2461 aaaaaatccc aacgtttcgt cttccatgaa aacaatgccg cgacatgcgc ggcattatgt
     2521 agccaggttg gcaaatttta gtgtcttcag ccacgtcttg cacgctggat gtcgcgtatc
     2581 cgctgttttt ctgcgcgcgc catcagatac caggcgataa atccgacaat tccgaccgcg
     2641 ccgaggatca atgttgccag ggcgttgatt tccggattca cccccatccg cacgctggaa
     2701 aagaccagca tcggtaaagt ggtggctccc ggcccggaaa caaagctggc gatcaccaga
     2761 tcatcaagcg acaaagtaaa agccagtaac cagccagaaa tgatcgcggg catgatcatc
     2821 ggtagcgtaa tgacaaaaaa cactttcagc ggcgtcgcac cgagatccat cgcggcttct
     2881 tctatcgagc gatccagttc ccgcagacgc gacgaaataa cgactgccac ataagccgta
     2941 cagaaagtga catgcgccag ccagatggtg agcataccgc ggtccgcagg ccagccaata
     3001 gcatgagcaa gcgcgacgaa taacaacaat agcgacaagc ccgtgatgac atctggcatc
     3061 accagcggcg cggtgatcat aaaggcaaaa ccatttgatc cgcgaaacct accaaagcgc
     3121 accagcacga ccgccgcaat cgtcccgagg atcgccgccg ccgtcgccgc acaggccgca
     3181 attgtcaggc ttaaaccaac cgcactcatc atcgcgttat cgcgcaataa ctcaccatac
     3241 cagcgtgttg accagccggc ccacaccgtc accagtttcg agctgttgaa tgaatagatc
     3301 accagcatca gcattggcgc gtagagaaag gtgaagccca gcagcaaaat cacaatccgc
     3361 cagggcgaac gaacaaccgg taaattattc atccgtgttc tcccacgctt ttttgctggt
     3421 gtttgtgaaa ccacattatc ggcacaatta gcagcagcaa catgatgatc gctaccgccg
     3481 aggccaccgg ccagtcgcgg ttattgaaaa actcctgcca cagcacgcgc ccgatcatga
     3541 tgctgtccgg gccaccgagc agttcaggga tcacaaactc gcccacagcc gggataaaca
     3601 ccagcatcga tccggcaata atcccgcctt tggtcagcgg cacgatcaca gtaaagaacg
     3661 ttttcagcgg tcgtgcaccg agatccagtg ccgcttccac cagcgaataa tcaatacgaa
     3721 tcaaggcggt gtagatcggc agcaccataa acggcacata ggcgtaaaca atgccgatat
     3781 aaaccgccag gttggtatgc agaatggtca acggttgatc gataaccccc agccacagca
     3841 gaaaattatt cagcacaccg ttgtttttta atattcccat ccaggcataa acgcggatca
     3901 gaaacgaggt ccacgacggc aggatcacca gtagtaataa gatattacgg gtcgaaggct
     3961 tactgtgcgc caccgcccac gccagcggat agccgatcag taaacagcaa aatgtcgaaa
     4021 tcgcagccac ctgtagcgac tggagataag catcgaaata gagcggatcg tcagtcagtt
     4081 gcagaaaatt accgagatta agagtgatgg acagttgccc gtcagcccac tccatcagtt
     4141 cggtataagg cggaatggcg cgcgccatct ccgccagact tattttaaag acgatcagaa
     4201 atggcagcag aaacagcagg atcaaccaga tatacggcaa cgcaatgacc agtttgcgcc
     4261 catgcttcat ttgcagctgc gacaaccaca gcttaaatcc gcccggcggt ttgggctggg
     4321 cagcaggttc aagtgtactc atctttgctc cttaaaccgt cagcaccaca cagctgtcca
     4381 cttcccagca caaacgcact tcgtcgcccc aggttggtaa ccctttgcga tatcgatgag
     4441 cgttttgtag ctgagcgctg atcatctgtc cgctcttgag acgaacgtga tacaccgaaa
     4501 gatcgccgag ataggcaatg tgcatcacct cacctaccgc aaagttaaaa ccgtcagcgg
     4561 gaggttcctc gcaaagcatg attttttccg ggcgcagcgc cacatgcacc ggcacattat
     4621 cgaccaccga agcatctaca tcgactttca gtggatgcac cagccccggc gaataaagca
     4681 ccagaccatc ttcctgacgc tctttgagta caccttcaaa gacattaacc gagccaataa
     4741 attcggcgct gtagcgagtg gtcggatgct cgtagatctc ttccggttcg ccaatctgga
     4801 caaatttccc tcgattcata atggcgatac gccccgccat ggtcatcgcc tcttcctgat
     4861 cgtgggtgac catcacacaa gtcacaccga cacgctccag aatatccacc acttcaagct
     4921 gcatccggtc acgcagcttt ttatccagcg cgcccatagg ctcatcgagc agtaatagtt
     4981 tcgggcgctt ggcaaggctt cgggccagtg ccacacgctg tcgctgaccg ccggaaagct
     5041 gatgcggttt gcgtttggcg aactcctgca tatgcaccag cccgagcatc tcattgaccc
     5101 ggctggcaat ttccgctttc ggtagtttgt cctgtttcag gccaaaagcg atgttctgtt
     5161 ccacggtcat atgcggaaac agcgcgtaag actgaaacat catatttatg gggcgcaggt
     5221 aaggcggaac ctgtgacaaa tcgacgccat caagcattat ctgtccggca gaaggttgtt
     5281 cgaaacctgc cagcatacgc agcagcgtgg acttgccaca gccggatgcg cccagcagcg
     5341 cgaagatttc gcctttatag atggtcaggc tgacatcatc gaccgcatgc tgaccatcgt
     5401 aagatttagt cagattacgg atttctaata gcggcgtcag caccttacgg gttttcgcct
     5461 gcgggcgagg gatagcgtca ttcacggctg ctctccggca ttgaatgtgt gcaagcgcac
     5521 cataatggtg cgaattgccg gcgggcgtgg tgcgcccgtc cggcatctac gactgcggat
     5581 tattttccgc tcttcacttt ggtccacgcg cgggtgcgca cacggtcgat tttcggatcc
     5641 tgtactttca gagtgaacag cttcgcacga acatccgcag gcgggtaaat acccgggtta
     5701 tcacggactt ccgcactcac cagcggcgta gctgctttat tggcgttagc atagaacaca
     5761 tggtcggaaa tatgcgccac cacatccggg cgcagcaggt aattcaggaa ctgataggct
     5821 tcgtctttat ttttggcatc cgcaggcatg gcgaagacat caaagaacgc catcgcccct
     5881 tctttcggaa tcgagaacga gacattaacg ccattcttcg cttccttcgc gcggtttgac
     5941 gcctgccaga catcgcccgc ccagccaata gcgacgcaga tatcgccgtt tgccaggtcg
     6001 ttaatgtatt gagatgaatg gaaataacga atgttcgggc gcagctttaa cagcagatcg
     6061 gttgccggtc cggtgtaatc atctgcttta gtgctgttgg gatctttacc gagataattc
     6121 agcacggtag caaacacttc ttccggtgca tcaaggaaag agacgccgca gcttttcagt
     6181 ttttccagat tttcaggctt gaggatcaag tcccagctat cgacgggcgc gttttcgccc
     6241 agcaccgctt taactttatc aacgttatag ccaatcccgg tcgtcgccca catatagggc
     6301 atagcaaatt tattgtcggg atcgtgtttg gcaactaact tcagcagctc cggatcgaga
     6361 ttcttccact ccggcaattt gcttttgtcc agcggctgga aaactcccgc agtcaactgg
     6421 cgttcaagaa agcttgctga cggcaccacc aggtcaaagc cggtgctacc cgccattaat
     6481 ttgccttcca gtacttcgtt agagtcgaaa acatcgtaga cgactttaat accggtttct
     6541 ttttcaaaat tggccaccgt gtccggggcg atataatcag accagttata aatgtgaagt
     6601 gttttttgtt cagccgcgag cgtgccgaca gagacggcca tcagagcacc cgtaaccaga
     6661 cccgatagcc attttttatt taaggcggtc atatctcttt ccttctgaaa gttcgttaac
     6721 aaacgaacaa aagttgtcac actgaaagat atgcaataaa cgtgcatatt ttttatcggc
     6781 gcggtgtaag aaaagagaac tctctcccgg ccagcactgc tcgctatata aaagcatcgc
     6841 aagaataact ttagccaggg tttgagacta tagctcggat taaaaaacgg tatttaacaa
     6901 atttttatgc gtatttgctc tatgtgataa agccaaatcg gcacaagaga agtgaaataa
     6961 tctttaagaa aaggttaaga atagcagata attttgcagc acgcagtcac tgcggcagcg
     7021 actgcgtact cgaaaggagt tatcagtgga ggaaatagtt ttcggtaacg ttgttttgcc
     7081 cttcatcgtc agtcggtaac agcagttgat gggcattagc ttcaagaatg accatcgaaa
     7141 tctgctcttc gctctgacgc acgaaccatg caaactgctc ataggttacg ccctgcataa
     7201 cggataaaga ctggcaaacc accagctttg gcagattatc atcctgcata tcaagaaatg
     7261 ctttcacggt cagcgaactg gcattgatgg cagataaatc cgccgccagc ggcaatacgg
     7321 ctgaaggtcg gacttccgcc atggcagaaa acaggatcgt gttatcgatc agatcgattt
     7381 tggcatcaaa gacaccgtcg aaattctgca tatggggcag atgcagagcc tgacagttat
     7441 cacattcaaa aaaactcatc cccaggtcat cgagccattg acgcagcgta tccagaccag
     7501 gaacgaccag cgatgtcata attttgtaca acctctttcg ataaaaagac cggcacagct
     7561 tacgcaaaaa gcgcaggcaa aaccatgatc agtaatgtga ttgcgactaa ccacccgttt
     7621 tcaggcaata ttccgtcgta gcgtggcgtt cgatccagcg gatcatttta cccgcgatgt
     7681 cgataccggt ggttttttct attccttcca gccccggcga cgcattcacc tccatcacca
     7741 atggcccgcg attagcacgc agaatatcca caccagcaac gtccagcgcc atcgttcgcg
     7801 cggcttttat cgcgatttca cgctcctgtg gtgtgatact ggcgacgctt gccgcgccgc
     7861 cacgatgcaa attggaacga aaatcgccct ctttcgcccg ccgttcaatc gcagcgacca
     7921 cttcatcgcc aacaaccaga cagcggatat cgcacccttg cgcctctttg atatattcct
     7981 gcaccagaat atgcgcgttc agaccgcgga aagcgtcaat cacgctttcc gccgcctggc
     8041 gcgtctccgc cagcacgaca ccaattccct gcgtgccttc aaccagcttg accaccagcg
     8101 gcgcaccacc gaccatgtcg attaaatcgc tggtatcatc cggcgaatgc gcaatgcccg
     8161 tgacaggcag gtcgatgccc tgacgcgcca gcagttgcat ggaacgcaat ttgtcacgcg
     8221 cccgggcaat ggcgaccgac tcattgagcg gatagctccc cagcatctcg aactggcgca
     8281 gtgccgccgt cccataaaag gtaatggcgg taccaatacg cgggatcaca gcatcaaaat
     8341 ggggtaactt gcggcctttg tagtgaatag aagacgccgc aggatttatg ttcatgtagc
     8401 aagaaagcgg atcaagaatt tcaaccaggt gaccgcgctg tatagcggct tcacgcagcc
     8461 gcttacacga atagagcgtt ccatcccggg acaatatggc aattttcacc ctgcacctct
     8521 ctgtcagacc tggtcaaaag cccgcgtatc atacatcggc gtggcggttt tagcgcgtcg
     8581 cccaaccctg tttgtgcaaa taatccagta taaatgggcg gctttcttta atgattgttc
     8641 ggcggatatg atcgctccag gtatcccggc gattattgct gccacgggtg aggtaatatt
     8701 ccgccagttg ttcgtcatac tgcgccagtg cgtctttatc cagcggttga tagttgtttt
     8761 catgcaccaa aatggaggcc ggtaaacgcg gcttaagatc cggattatcc gcaggccagc
     8821 caaggcacag cccaaacagc ggcagaacat gctgcggtaa tttcagcagc tccgtcaccg
     8881 cttcaatatt attgcgcagg ccgccgatat ataccccgcc caatcccagc gattccgctg
     8941 cggttaatgc attctgcgcc atcattgccg tatcaacgac accgagcaac agttgttccg
     9001 ccaggccaag ttgtgcatcc ggacagatct gtaaatggcg gttaaagtcg gcacagaaca
     9061 cccagaactc cgccgcttgc gctacgtgtt tttgcccgcc ggtcagcgtc accagttcat
     9121 cacgtaacgc tttgtcggta atgcgaataa tgctactgca ctgcaaaaaa ctggaactgg
     9181 acgtcgcacg ggcgctgtta ataatcgcct cacgctgcgc ttcggaaatg ggttcatcag
     9241 tgaaatggcg aatggagcga tggccacaaa taagttcaat ggttggcgtc attatctttt
     9301 tctctttctg aacgtgaata ttgcggtgga cggttcatca gctgtggggc aagacgtttt
     9361 gccacctgaa gaataaccac caccgcagcg ggaagcatga gcaaaacacc gagaaaaatc
     9421 atcagaatct gcacttctgg ccgagaaaat ggctcaggca gcgacaggga gccgcttacc
     9481 gacagcagcg ccaccgccag caacatcatt ccgataaatt ccagtatcaa cacgctttta
     9541 ggcaatttac cgatcgcgcg catacgcttc cctctgcaaa gtgagccttc agtctaaaac
     9601 ttttcactgt attgtgttta acagttatag cttttagcaa ttaatgcaac aggttaaacc
     9661 tactttcagc gaatacattt tagcgtgatc attacaggta taaatctatg aggagagaaa
     9721 taatgcaaac cgttattttt ggtcgtccgg gttgccctta ctgtgtgcgt gcaaaagatc
     9781 tggctgagaa attgagcaat gaacgcgatg atttccagta tcagtatgta gatattcgtg
     9841 cggaagggat cactaaagaa gatctacaac aaaaggcagg aaaacccgta gaaaccgtgc
     9901 cgcagatttt tgtcgatcag caacatatcg gcggctatac cgattttgct gcatgggtga
     9961 aagagaatct ggacgcctga tcgtctgaca agccctcgcg ttgagggctt tattgatttt
    10021 ttctgtgctg tggtttaaac aaactactga taaataagaa acacaatgcc cccagcgcac
    10081 accagaacac cgcacttaat aaccatgcca gctcttgcca gaatgagcgc gtcggtgaaa
    10141 aaaacagccg cataatgagc atcgaacagg gtgccgccag cattgcgcca aacagaggtt
    10201 tcaggacttc tctacgatgt gaaaagaagc tggcaactgc accaggaaga atgaaaaata
    10261 gcaaaccgat ttcaggatgc ccggcagccc gaaaagcgcc tttcatgtgc gtcgccagaa
    10321 aaagacacac cacaatgaag aggacaaaac agcagattgc ccccgcccaa cgttgtttat
    10381 gtttcactcg ttcctcctga cactgcgtct atcgaacaca tttttcgcca gtgtggcgtt
    10441 cagtaagata aagccgcttc gcattccatg ctaatatagg ccaacgcaat tcatatagcc
    10501 gttgatacct aatgtgatta cactagtaaa atatattgtt actttactat cgtttaggtg
    10561 cgctgaatga atctgcgccc tgaattctgg taaaaaacat tatcgtaaat taccatttct
    10621 ttcaacagct tactagtaaa caagaagtta gcctccgtga atataaacgt cgccgaattg
    10681 ttaaatggga attacattct gttattattt gtggtcctcg cgcttgggct atgtctcggg
    10741 aaattacgac ttggttcgat ccaactgggt aattccattg gcgttttagt cgtatcgctg
    10801 ttattaggcc aacaacattt cagcattaac accgacgcgc ttaatcttgg ctttatgctg
    10861 tttattttct gcgtcggggt cgaagccgga ccgaactttt tttccatttt ttttcgcgat
    10921 gggaaaaatt acctaatgtt agcactggtg atggttggca gtgcgctggt catcgcctta
    10981 gggttaggta agctgtttgg ctgggatatt ggcctgacgg ccggtatgtt agcaggctct
    11041 atgacgtcaa caccggttct ggtcggtgct ggcgatacac tgcgtcattc cggcatggaa
    11101 agcaggcagc tctcattggc actggataat ctgagcctcg ggtatgcctt aacctattta
    11161 atcggcctgg tgagtttgat tgttggtgcg cgttacttgc cgaaattgca gcatcaggac
    11221 ttacagacca gcgcccagca aatcgcccgc gaacgtggcc tggacacaga tgccaaccgt
    11281 aaggtttatt tgccggtgat ccgcgcctac cgcgtcggcc cggagctggt agcctggacc
    11341 gacggcaaaa atctgcgtga actgggtatt tatcgacaaa ctggctgtta cattgaacgt
    11401 attcggcgta acgggattct ggcaaatcca gacggtgatg ccgtactaca aatgggcgat
    11461 gaaatagcgt tggtaggcta tcctgacgcc cacgcccgac tcgatcccag tttccgtaat
    11521 ggcaaagaag ttttcgatcg tgaccttctc gacatgcgta tcgtcactga agaagtggtc
    11581 gttaaaaacc ataacgctgt tggcaaacgc ctggcacaac tgaagttgac cgatcacggt
    11641 tgcttcctta accgcgtcat acgtagccag attgagatgc cgattgatga caacgtcgtg
    11701 cttaacaaag gtgacgtttt acaagtcagc ggcgatgccc gccgggtaaa aaccatcgcc
    11761 gatcgcatcg gctttatctc gattcacagc caggtcactg acttgctggc attctgcgcc
    11821 ttctttgtta ttgggctgat gatcgggatg atcaccttcc agttcagcac attcagtttc
    11881 ggtatgggga acgctgccgg gttgttattc gccggaatta tgctgggctt tatgcgtgct
    11941 aaccacccga ccttcggtta cattccgcag ggtgcattaa gcatggtgaa ggagttcggc
    12001 ttgatggtgt ttatggcagg cgttggtctg agcgctggta gcggtattaa taacggcctg
    12061 ggcgcgattg gcggtcagat gttgattgcc ggattgattg tcagtctggt gccagtggtt
    12121 atctgcttct tgttcggtgc ttatgtattg cgaatgaacc gcgcactgtt gttcggcgca
    12181 atgatgggcg cacgcacctg cgcgccggca atggagatca tcagtgatac agctcgcagt
    12241 aatatcccgg cactgggcta tgcgggcact tacgcaatcg ccaacgtcct gctgacgctg
    12301 gcagggacaa tcatcgtcat ggtatggcca ggattaggat aaaactgaag ttgccctgaa
    12361 aatgaaattt tttttgcaca accgcagaac ttttccgcag ggcatcagtc ttaattagtg
    12421 ccactgcttt tctttgatgt ccccattttg tggagcccat caaccccgcc atttcggttc
    12481 aaggttgatg ggttttttgt tgtctaaaat ttatctacta ccctgcaacc ctctcaacca
    12541 tcctcaaaat ctcctcgcgc gatagcggct tacggtcggt gacaaaatgc agcgtcatcc
    12601 cctcaataaa cgcatcaagc gcgcgggcgg ttccgggttc aaaccattgt tcgagcgttt
    12661 gctgactgcg ctgcatccag ttttgcatta ccgtttttaa tagcggtttt cggctagcca
    12721 gcgcgtagag ctggtacatc agctccatgt tatccggcgt tgcaacctgt gagctgtaaa
    12781 tcatatcggt gatagcctgg catgcgcccg gagcatcact aacatcgcta aaaaatgcct
    12841 gatattgccg ggacatgatc tcagtaaaac tgctgaacgc ctccagcaac aactcatcaa
    12901 ttcctgaaaa atagtaggtc atcgacccca acggtacccc ggcaagggta gcaattttgc
    12961 ggtgcgtaac agcatgtatt ccataaagtt tcaccgcctc cagcgtggcc tggataattt
    13021 tttctcgccg ttgcggatcg ttagcgcgac gcatgtcatt tcctctctct gcaattgtgt
    13081 acaaatgtac acaaccttgc taatgttgtc ttccctcttc ttattttgac gtgatctatg
    13141 accgtaaatt cttcacgtaa tgcattgaaa cgccgaacct gggcgctgtt tatgttcttc
    13201 tttttgccag gcctgttaat ggcgtcctgg gcaacccgta cgcctgctat ccgcgatatt
    13261 ctttctgttt cgatcgctga aatgggcgga gtcctctttg gtctgtcgat cggttcaatg
    13321 agcggtattc tctgctcggc gtggttagtg aaacgctttg ggacgcgtaa tgtcatcctg
    13381 gtcacgatgt cctgcgcatt gatcgggatg atgatactaa gtctggcact ctggctgaca
    13441 tcgcccctac tcttcgcggt tggtctcggc gtctttgggg caagttttgg ttctgcggaa
    13501 gtggcgataa acgttgaagg tgctgccgtt gagcgagaaa tgaataagac ggttttgccg
    13561 atgatgcacg gtttttatag cctgggcacg ctggcaggcg ctggtgtcgg gatggcacta
    13621 acggcctttg gcgttccggc aacggtgcac attttattgg cggcgctggt aggcatcgcg
    13681 cctatttata tcgccattca ggcaatccct gatggtacgg gcaaaaatgc tgccgatggc
    13741 acccagcatg gtgaaaaagg cgtacctttt tatcgcgata tccagttgct gctgattggt
    13801 gttgtggtgc tggcgatggc ctttgccgaa ggttctgcca acgactggtt acccttatta
    13861 atggttgacg gtcacggttt tagccctact tccggctcgc tgatttatgc cggttttacc
    13921 ctggggatga ccgttggacg ctttaccggc ggttggttca ttgaccgtta cagtcgcgtt
    13981 gccgtggttc gggccagtgc actaatgggg gcgttgggta ttgggctgat tatttttgtc
    14041 gatagcgcct gggttgctgg ggtgtctgtt gtactttggg gactgggtgc ctcgttgggc
    14101 ttcccgctga ccatttctgc cgccagcgat accggccccg atgcaccaac ccgcgtcagc
    14161 gtggtagcta cgaccggtta tctggctttc ctcgtcgggc cgccactgct gggctatctc
    14221 ggcgaacatt atggattacg tagtgcaatg ctggttgtac tggcgctggt tattctcgcg
    14281 gctattgtcg cgaaagccgt cgccaaaccc gataccaaaa cgcagacggc gatggagaat
    14341 agttgatgag cattaaatta attgcggtag acatggatgg tactttctta agcgatcaaa
    14401 aaacctataa ccgtgagcgg tttatggctc agtatcagca aatgaaagca caaggcattc
    14461 gctttgtggt cgccagcggg aatcaatatt atcagttgat ctctttcttt ccggaaattg
    14521 ctaatgaaat agcctttgtg gctgaaaacg gcggctgggt agtgagcgaa ggcaaagatg
    14581 tttttaatgg cgagctgtcg aaggatgcgt tttctactgt cgtggaacat ttgctgacgc
    14641 gcccggaagt ggaaattatt gcctgcggaa aaaatagcgc ctatacactc aaaaaatatg
    14701 acgatgccat gaaaacggtg gcggaaatgt attatcaccg tctggaatac gtcgataact
    14761 ttgacaactt agaagatatc ttctttaagt ttggtctgaa tctttccgat gaactgattc
    14821 cacaagtaca aaaagcatta catgaggcca tcggcgatat tatggtgccg gtccacaccg
    14881 gcaacggcag catcgatctg attatccccg gcgtacataa agccaatggc cttcgccaac
    14941 tgcagaaatt atggggaata gacgacagcg aagtggtggt ctttggcgat ggcggtaacg
    15001 atattgagat gctgcgtcag gcaggcttta gttttgcaat ggaaaatgcc ggcagcgcgg
    15061 tcgtcgcagc ggcaaaatac cgggcaggct ccaataaccg tgaaggcgta ctggatgtga
    15121 tcgataaagt gcttaaacac gaagcgccat ttaaccaata aaacggcagc acgcccggca
    15181 acacaatacc tgaatgaagg ctgcataatt cgcggaaatt aacccgctgc attatttcag
    15241 gcgaaatgat tatgaaaaat tgtctactgt tgggcgcgct tttaatgggc tttactggtg
    15301 tggcgatggc gcaaagtgtc accgtcgatg tgccgagtgg atacaaagtg gttgtggttc
    15361 ctgattcagt cagcgttcca caagcggtca gcgtcgcgac tgtgccgcaa accgtctatg
    15421 tggctcctgc gccagctccg gcttaccgcc cgcatcctta tgttcgccat cttgccagcg
    15481 taggtgaagg gatggttatc gaacaccaga ttgacgacca tcaccattaa ttcgagaatg
    15541 cctgatcgca caaagcaatc aggcattttt ttacccttcg tgcgaatttc ccatctgttt
    15601 atcttttaaa aagataacca tcagcaacag ccacaaaatt ccgttgacaa ggttgaagag
    15661 attaaacagt ccgttgcccc cgttcagcca gacatgtttg ctgatttcaa taccgacggt
    15721 aaagatcagc atttgcagca tccccatcgc cgcagaaacc gtacctttac tcatatcgct
    15781 ggcaaacagg gttaatcgca ccagtcccgc attcgccaga ccaataccga aagcataaat
    15841 acttaacccg gcggtcatcc ataaatacgc gtgcgatgag ataaccgttg ccgcagcagc
    15901 gaccaacaga ccaatcataa tcggccagcc gcccataata atcagcgaac gtacggtgcg
    15961 gcgcgaggtc agacgcgcta acagcaagtt acccgcaatt aacgccccga aaataggcac
    16021 ttgcagcaag ccatattcat agctgctcaa ctgctcaccg gtaataatga taatcggcga
    16081 ctgggcgatc cacgccaaca acggcaggct aacgaatccc agcgccagcg cccccgccac
    16141 aaagcggccg ttcttcagca ccagcttata gtcacgaccg agttctttca gcgacagttt
    16201 ctcgcctata cgcgtggcgg tttcaggcat ggctcgttgc agaccgaaaa aggagatcgc
    16261 tgccaatgcg gcaaacaaga caaacatccc ttcccagggc agcacatgga tccacgccgc
    16321 gcccaccagc ggaccaagta gtggagcaat cagcgccacg ttcgccatta gcgcggtgat
    16381 cttgatacaa accgcctctt cgaaggattc ctgaattgcg gcgtatccca cagcgccaat
    16441 gaaacagagg cttatgccct gcaagaagcg caacagggtg aattgttcaa tgttttgcgc
    16501 cagtaatatt gccagacagg tgacgataaa ccacaccact cccgccagca tcaccggacg
    16561 gcggccaata cgatccgaca acggccccag gagccattgt aaaaacatcc cgcccgccag
    16621 atacgcagtc atcgaagtag gaacccaatc aatgcccgcc tgatattgtt ccaccacggc
    16681 caacataccg ggttgaatca tatcgttgcc gatataggtt gaaaattcgt aaagcaccag
    16741 acagagaggg aaaagtaacg cctgacgtcc aagcctggca ccggaagcta atttattttg
    16801 catgcaattt cttcgccaat aataatcgcg caaagtttaa taaaagcgca gctaacgaga
    16861 aagcgaattt tgtagctgaa accacggtta agcacattct tacattattg cgagtatagc
    16921 tacgctttct ttaagtttta tttaaccttt gcccgttaca atcacccacc gtaaacaggc
    16981 cgcttgaggg aaataagacg atgccgcttt acccagttta acctgcactt tattctcaac
    17041 gacttgcctg tattggctcc cttttaatca ctttgcgtcg ggaagttatg ctggaaaatt
    17101 tgaatctctc tctattctct cttattaacg cgacgccaga ctctgcgccg tggatgatct
    17161 cgttggcgat ttttattgca aaagatttga ttaccgtggt gccgttgctg gccgcggtac
    17221 tttggctgtg ggggcttaca gcacaacggc aactggtgat aaaaatcgct atcgcgctgg
    17281 cggtcagcct gtttgtgtcc tggtcgatgg ggcatctttt tccgcacgac cgaccctttg
    17341 tcgaaaatat cggctataac ttcctgcatc atgcggcgga tgactcattc ccgagcgatc
    17401 acggtacggt gattttcacc tttgcactgg catttttatg ctggcatcgc ctgtggtccg
    17461 gctcactttt aatggtgctg gccgtcgtca ttgcctggtc gcgcgtttat cttggcgtcc
    17521 actggccgct ggatatgctc ggtggattgc tggcaggtat gattggctgc cttagcgccc
    17581 agattatctg gcaagcgatg gggcataaac tctatcaacg tctgcaatcg tggtatcgct
    17641 tctgttttgc attaccgatc cgcaaaggct gggtgcgtga ctgatctctg ctaaaaagtg
    17701 tagtattgag cggctcgctt caataactat tcagagggat tatggaaaca cgtcgcgaag
    17761 agcgtatcgg gcagctgctg caagaattaa aacgcagtga taagttacat cttaaagacg
    17821 ccgccgccct gcttggggtt tcggagatga cgattcgtcg cgatctgaac aaccacagtg
    17881 cgcccgtcgt tttgctcggc ggctatattg ttctggaacc acgcagcgcc agccattacc
    17941 tgttaagcga tcaaaaatcc cgcctggtgg aagaaaaacg ccgggcggca aaactggctg
    18001 cgacgctggt agaacccgat cagaccctct tttttgactg tggcaccacc acgccgtgga
    18061 ttattgaagc aattgataat gaaatccctt ttaccgccgt ttgttattcg ctaaatacct
    18121 ttctggcgct gaaagagaaa ccccattgcc gcgcgtttct ttgcggtggt gaatttcacg
    18181 ccagcaacgc cattttcaaa cccatcgatt ttcagcaaac gctgaataat ttttgcccgg
    18241 atatcgcttt ttattccgct gcgggcgtgc atgtcagtaa aggcgctacc tgctttaatc
    18301 ttgaagagtt gccggtaaaa cactgggcca tgtcgatggc gcaaaagcat gtgctggttg
    18361 tcgaccacag taaatttggc aaggtgcgtc cggcgcgcat gggtgacctg aaacgctttg
    18421 atattgttgt gagcgattgt tgcccggaag atgagtatgt gaagtacgcg cagacgcagc
    18481 gtattaagtt gatgtattaa tgacgtataa ccggatgacg tttcgcgcca tccggttatc
    18541 agaagattaa gagaaccagc tgccgaacca ctgatggaat ttcatcatca cgaaatccca
    18601 catccgacca aagaatccgc cctcttccac attttccatc acgatcagcg gacgctgctc
    18661 aatggattta ccgttaagct ggaaatcaat ggtgccgaca acctgacctt ttttcagcgg
    18721 tgcggtaagc tgcggttccg ttaacgtata actcgccttc aggtttttca gctgcccacg
    18781 cggtatggtc actgagcccg cttcgcctgc cccgagattc acttcgctct tatcaccaaa
    18841 ccagacgcgc tgagtcacaa atgtggcatc aggtttaatt ggcgtcacgg tttcaaagaa
    18901 acggaagccc caggtcaata atttctcaga ctcattaaaa cggatacggt cagttttcgc
    18961 ccccagcact acggagatta aacgcatatc gccctgggta gccgaagcaa ccagattata
    19021 tcccgcgcct gccgtggttc ctgtcttcat gccatcaaca ttcagattgc tgctccacag
    19081 cagacgatta cggttaggct gacgaatttt gttgaaggtg aactctttct ctttatgaat
    19141 ggcgtactct tccggcacat cgtggatcaa tgctttaccc agcaatgcca tatcgcgcgc
    19201 ggtactgaac tgccccggcg catccagacc gtgcaccgtc tggaaggtag tgttggtcag
    19261 acccagtttt ttggcataac cattcatcag accaataaat gactcctggc tcccggcaac
    19321 gtaatcagcc agcgcaatac aggcgtcatt accggactgg ataatcacac ctttgttcaa
    19381 gtctgccacc gaaacctgat cgcccggttt gaggaacatt accgatgaac cacgcagtgc
    19441 cggatttccc gtcgcccagg catctttacc gaccgtcacc atatcggtga gtttaatctt
    19501 atcggcctta agcgcctgcc caaccacata gctggtcatg attttagtca ggctcgcggg
    19561 gtccagtttt tcatccgcat tgccttctgc cagcacttta ccgctggcgt aatccattaa
    19621 aatccatgca cgcgcatcca cgctcggcgc ttcaacggtt tgttccgccg cgaatgccgt
    19681 tggggcaaaa aggaataaaa atgcagaacc cgctgcaaga ccacgaagga gagaggagta
    19741 ttgcgtcata aatgccaccc gagtatccat tcaaaaaaaa tacgttatat cgccgtatga
    19801 taagttcggt gattaataac gcacacacaa acttaaagaa accgtaagtt ggtaaagttt
    19861 ttaaagttta tgcaatatcc tgccgcttcg ctgcttctgg tgtcattttt ttgccagctg
    19921 ttgcttaaaa gttaacttta cttcaccatc ggagtcctgt tcagcctgtg gctgcctgtc
    19981 ggaaaagggg tgagtatgat tacgctgtgg ggtcggaata attcaaccaa cgtaaaaaaa
    20041 gtattgctga cgctcgaaga actggaacta ccttatgagc aaattctcgc gggccgtgag
    20101 tttgggataa atcacgatgc tgattttctg gcgatgaacc ctaacgggct ggtgccgttg
    20161 ttgcgtgacg acgaaagtga tctcattctt tgggaatcaa acgccattgt ccgctatctg
    20221 gcggcacagt acgggcaaaa acgcctgtgg atcgactcac cggcacgtcg tgcggaagcc
    20281 gaaaaatgga tggactgggc aaaccagacg ctcagtaatg ctcatcgcgg gatcctgatg
    20341 ggattagtca gaacaccacc ggaagagcgc gatcaggccg ccattgatgc cagttgcaaa
    20401 gagtgcgacg ccctgtttgc cctgctcgat gcggaactgg caaaagtaaa atggttctca
    20461 ggcgacgagt ttggtgtggg cgatatcgct atcgcaccgt tcatctacaa tttgtttaac
    20521 gttggcctga cctggacacc gcgtccaaat ctgcaacgct ggtatcagca actcactgaa
    20581 cgccccgcag tgcgcaaagt ggtgatgatt cccgttagct aattgcgtgg gctaacttta
    20641 agtaattccc cactggactc gtcggtgaga acgtataaat aaccgtcggg tccagtgcga
    20701 acatcacgaa ttcgctgccc tctgtccgtt aaaatacggc catcttctgt cactttgtcg
    20761 ccgttgacgc tcatcacaat gacatcttta tctttcagcg cgccaataaa taatttttgc
    20821 tgccactggg ggaatttgtc gctgttatag aaggccatgc cgctcacggc gggcgaatct
    20881 ttccagtaaa aaacaggttg ctcggtcccg gcgacgatct cccctttcgc ctccggtatc
    20941 ttaaagcctg aatagttgat tccccaggtt gccagcggcc agccgtagtt tttgcctttt
    21001 tgcgggatat taatttcatc gccaccgcgc gggccatgtt cattcagcca tagtgcatta
    21061 ctccacggat tcatcgccat tccttgcgga ttacgaatgc cataagacca gatctcggcg
    21121 cgtgcatcgg attcctttat aaaaggatta tcatccggga tttctcccag gtcggtcagt
    21181 cgcaccagtt tgccctgtaa tttatccaga tcctgcgccg tcgggcgctg attgttttcg
    21241 cccagagcaa taaaaagata acctttaccg tcaaatacca tccgcccgcc aaaatggttg
    21301 ccggtagaca gttttggcat ctgacgaaag acggtgcgga agtcggtcac ttttgagaga
    21361 tcatcactta agcggccata acccacagca gttccggctt tgccatcatc gccaacttcg
    21421 gaataactta accagatgcg gcgcgactga gcaaaatcgg gcgctaaaac cacgtccagc
    21481 aggccgccct gcccgtgcgc ccaaacgtcc ggcactccgg aaagcggcgc agataatcct
    21541 tttcctgctt gccagtgacg caactcgccg ccgcgcagag tgattaacat accgtgattg
    21601 tcgggtaaaa aggccagcgc ccagggatgg tcgagtttgt cctgcagtac ttcgacattt
    21661 accgttgcag gagccgccca gagagcggaa gaaagacaaa taaggggcac aaggaaaaag
    21721 gattgtcgat gcataacgcc tccggattga ctctggctta aagcgtagtc agtggaggag
    21781 ataacaaatt tatttttaca aaaacttaaa catgaagggg gagacgcttt ctccccctta
    21841 gttttcaggc cttctcaagc atggcgtgct tctgcaggct ctggatactc agcgttaagc
    21901 tcatcagaca attttcaagc ttatcggcgt tgacggtaat aacagtcggg caatcatggt
    21961 gcccactcat caaacatact gcggctgtcg ctaatgcttc ttcagcatga tgaagagcac
    22021 tccactcttc ctgatccaga tgaagattca accgcagcga tttatcgtgc agttcgcgat
    22081 tcagtttaaa aaagttatct cgtagatgat tgctttcgct gacggacatg tatccttttg
    22141 ccttcctcag ttgcacgtat gcggcgaggt caacgcgtgc gtcgatcaac cggttcagca
    22201 gatcgattcg ctgtctgtca acgaacatac ttcgatcctc ctctttccag ccttgttgca
    22261 attaaatagt atggctattt tttgagcaaa atgctgtcta ggatcaattc ttcacgccga
    22321 attaacaaaa tttacatttc cttcgcctcc gccagcgaat gagattgcga tacaaatttt
    22381 atccagaact ctgtagaatc cctgccctga cctttactgt aactgaatat tttttaagct
    22441 atgagcaaag taactcccca gccgaaaatc ggctttgttt cccttggctg cccgaaaaac
    22501 cttgtcgatt cagagcgtat tctcaccgaa ctacgcactg aaggttatga cgtggtaccg
    22561 agctatgacg atgcggacat ggtgatcgtc aacacctgcg gctttattga cagcgcggta
    22621 caagaatcac tggaagccat tggtgaagcg ttgaatgaaa acggcaaggt aattgtgacc
    22681 ggttgtctgg gggcaaaaga agatcagatc cgcgaagtcc acccgaaagt gctggaaatc
    22741 accgggcctc atagctatga gcaggttctg gagcacgttc atcactacgt gccaaaaccg
    22801 aaacacaacc cattcctgag cctggtgcca gaacaaggtg tgaaactgac gccgcgtcat
    22861 tatgcctatc tgaaaatttc tgaaggctgt aatcaccgct gcaccttctg cattattccg
    22921 tctatgcgcg gcgacctggt gagccgtccg attggcgaag tgttaagtga agcgaaacgt
    22981 ctggttgatg cgggcgttaa agagattctg gtgatctcgc aggatacttc cgcctatggc
    23041 gtcgatgtta aacatcgtac tggcttccac aacggcgagc cggtaaaaac cagcatggtc
    23101 agcctgtgcg aacagttatc gaaactgggg atctggacac gtctgcacta cgtttaccct
    23161 tatccgcatg tggacgacgt catcccactg atggcagaag gcaaaatcct gccctatctg
    23221 gacattccgt tgcagcacgc cagcccgcgc attctcaaac tgatgaagcg tccgggttct
    23281 gtagatcgcc aactcgcgcg catcaaacag tggcgcgaaa tctgcccgga actgacccta
    23341 cgctcaacct ttattgtcgg cttccctggc gagacggaag aagatttcca gatgctactc
    23401 gacttcctga aagaagcacg tctggatcgc gttggctgct ttaaatacag cccggttgaa
    23461 ggtgcagacg ccaatgccct gcctgaccag gttccggaag aagtgaaaga agaacgctgg
    23521 aaccgtttca tgcagttgca gcagcagatt tccgccgagc gcctgcaaga gaaagtgggc
    23581 cgtgaaattc tggtgattat cgacgaagtg gacgaagaag gtgcgattgg tcgcagcatg
    23641 gcagatgcac cggaaatcga cggcgcggtt tatctcaatg gtgaaaccaa cgttaagccg
    23701 ggtgatatcc tgcgtgtgaa agtcgagcac gccgatgagt acgatttgtg gggtagccgg
    23761 gtttaattaa tcttaaggca gatctaacaa ggatattgtt cttcgatctg cctctgctac
    23821 ccttcccgat gtcctaccct tacgcacaaa atcccctgac gcataaatcg agaaaaatga
    23881 atcgtactcg aggcaactct cactttttca caccaaatta aagttttgat cgcaattact
    23941 tgaaataagc gtaatggtta ctatagtaaa cattaccaca aggatggatg tatgggtgtg
    24001 tataaggcaa gacgattcag ccaaagcacg aaaaaacttg gtattcacga taaagttctc
    24061 atggctgccg ctgaagaagt gattcagggc atatgggagg ctgatttagg tagcggtgtc
    24121 atcaaaaaac gtttacccct ccaacaagga aagagtggtg gagccagaac aattatcttt
    24181 tttaaatcag caaatcatgt gtttttttat gatggttggt ccaagagtgg tctaagcagt
    24241 aaaggaacca aagaaatcga agatgatgaa ctggcggcct acaaaaaaat ggcaaatgca
    24301 tttctggcct ttagcaataa gcaaatagag gatttgatcg aaactggctt tttgattgag
    24361 gtgaaaaatg agcgataaat tacaaatgat gctggaagat gcaaaggaac tgaatgcact
    24421 gggcctgatg tctgatagtg aagttgatgc cattgccgtc atggtcaaag ctcgtgaagt
    24481 cagcgcccgt atagccaaag tcaaatcaat gacgggtgaa gagatcaaag ccatccgcac
    24541 ccgatacggc atgtcacaat ccgtgctggc acatacaatg ggaatgtcaa aagaaagtgt
    24601 ctcgaaatgg gaacgtaatg aaataaaacc gagcggcccg gcattacgta ttttgaatac
    24661 attggcaata aaagggccgg aagtttttgc aaagtaaaat gtttgcaaat gatttaccac
    24721 attcattagt accccttgat taaggggtac taacactatt ctaattctac tgccagctaa
    24781 tctgcgccct gccgtttttc ttactcttat acagtcgctc atccgccagc attttcaagc
    24841 gttcgacggt agtacattca tttgaatgcg ctacaccgat actggtgctt aaattaatgc
    24901 ttcccacctg gaaatgaaca atttctttta gaatcgatag caactcttcc agcgttccac
    24961 ccgtataaat caccgcaaat tcgtcaccgc cgaagcggta gactttacct ttatctacgg
    25021 ggagctgtga tttaaggatt ttcaccactt tcatcaacac ttcatcgccc agcaaatgac
    25081 cgaaggtgtc gttaatgttt ttgaatttat cgacgtcaaa caggcacaaa taaccgcctt
    25141 tttccggcag cgcttttaaa tcttcgtcaa aggattttct gcccatcgct ttggtcagat
    25201 cgtccaggaa tgccgagtta tacatactgt agagtttttt gacatcggca gccgaaaaga
    25261 aaagcccgcc atccagtacg cgttcaaaat ggttattaat aatggcttct ttctccacaa
    25321 taccacgatt aatcactctg cgtaccagat agaggcaact ggcacccgat atcactgcca
    25381 ccaatgcggc aaagaaaata atcaatgtac atacctgaaa ataaacgtct tttttataag
    25441 caaatacgta caacccggtc ggcaaatagg cgaagtgctc catcgcttcc gaaacagtga
    25501 agatggcgtt tttagcgtta tattcatcac cggtcaccga tttaccgttc ttgaacaggg
    25561 agaacatcag gttctcgcta tccatcgcca gcgattttga cagtaaagaa aaacgttcca
    25621 gaccaagcaa aaatatcaga ctacgattgt gagttaccgg atagaggtaa tagacgaagg
    25681 ttttaccatc aaaagagata aaccctgtat ttttgacatg tcgatacttc acaacctgca
    25741 taatatctgc acaataattt ttctcgaact cctttacctg aatacattcg tcactggaaa
    25801 tcagataagg accgacatcg tacaaataag agggtgaaac cgagtcggcg taagctgtgc
    25861 gtaagccaac caacagctgt tcctccgtgg gatttagcgt gttgataaga tcaattgcca
    25921 cgctacgccg atattcagca atatttgcca ccgcaaaagc agtatcaata acggactgtt
    25981 tcaggctata cacccgttcc tttaccattt gagtgtagat cccgcaggca actgctgaaa
    26041 taatgataaa aatcagcaga ctgactgtaa gtacgaactt attgattctg gacatacgta
    26101 aactactctt ttactaattt tccactttta tcccaggcgg agaatggcat gggtttctgg
    26161 tacagatacc cctgagcctg aacacatcct aatttgatta attcgcgcgc aacgtcttcg
    26221 ctctctacgc cttccgcaat caccgggatc cggttatagc gtgccagatc gaaaataatg
    26281 cgcacaattt ttcccgcagt accaaaaata tcgccggtaa actgcgagtc cacctttatg
    26341 ctgtctggat taatttcata aaaacgtttc aggtttgaca aaccggtgcc aaagtcatca
    26401 atcgatatcc gtaccccttg tttacgcagt tgttcaatat tgagataaaa atgttcatta
    26461 attcgctcaa tcacggtttc agtgatctca acacaaaggc tgttcgcctt caggccattt
    26521 tctttcatga tggtagtaaa acgctcatga aagtgtggtt cgtttaactg ccagggcgaa
    26581 acattaatat gcagtaagaa atcatccttc agcgacgcac gctgactaaa atgtctgaat
    26641 tcacccatcg cggttttaat aatttgatag cccagctcat tgataaaccc gatatcttca
    26701 gcaatggtaa tgaacttcag cggtgaaatg atcccccgct ccgcagacac ccaacggcac
    26761 aacgcctccg cgcccagaat ggcgcgggtg ttaatatcaa cgatcggttg atagacgagg
    26821 aatagttcgc caatttccat tgcatggcga atattacgcg caatccagat attctcttct
    26881 atttcgttga gtttcgcatc atcacagaaa ataaactctg agaacatatg gttgcggata
    26941 ctggaaatcg ccagtttcag cttttctgcg taactccagg cgttttcccc ctgcaaaggt
    27001 tcacaaatag cagacttatg gatgcgcaga ttgcggtagc caaaactgct gtcaaaatcg
    27061 tttaccgtcg ctatcttacg ggtaaacgtc tgtacatttt cccggcacag gagtaaatag
    27121 agatcggcat taaatgtcac gatatcgcag cattgcgcat atttctcggc aattcgctga
    27181 cacaatttcc tgacgatttt attgcgttca tcaacaccaa acaccgcttc cacagcttcc
    27241 agaccacaca gttagatgat ccccacagcc agatactgat tattgttgga ttcaataatc
    27301 gccatatttt taataaaacc gctttcctga tcgtaagaga tcagtgagag caatttattt
    27361 gaccgttcaa tttcggcatc tttcatttcc gcgatcgaat ttttgatgct gataatctct
    27421 ttcgcatcgc cttcgcgaat tttcagagcc tcgatctgat ccgttgagtc gggcaaaaac
    27481 gccagcgtat taaccagctc ggtcatttca gaaaattgcc gacggaacac acgagtggtc
    27541 attaaataaa ccaccaacaa tacgataata atgaacgcaa acggcagata aaaaagttta
    27601 tctga
//
LOCUS       DAZFLD010000044        27574 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.044, whole genome shotgun sequence.
ACCESSION   DAZFLD010000044 DAZFLD010000000
VERSION     DAZFLD010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27574
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.044"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..298
                     /locus_tag="ACHKBL_001575"
     CDS             <1..298
                     /locus_tag="ACHKBL_001575"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016241930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PIG-L deacetylase family protein"
                     /protein_id="HFO5818459.1"
                     /translation="DRHQDHLAVYQASMVACRAIPQILGYETPSTWLSFMPQVFESVK
                     EEYFSLKLTALKKHKSQSQRDYMRPERLRAVAQFRGQQVNSDLGEGFVIHKMIL"
     gene            308..1504
                     /gene="yaiP"
                     /locus_tag="ACHKBL_001576"
     CDS             308..1504
                     /gene="yaiP"
                     /locus_tag="ACHKBL_001576"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFO5818460.1"
                     /translation="MKTWIFICMAVAILLWFLSTLRRKPSQKKGCIDAIIPAYNEGPC
                     LAQSLDNLLRNPYFCRVICVNDGSTDNTEAVMAEVKRKWGDRFIAVTQKNTGKGGALM
                     NGLNYATCDQVFLSDADTYVPPDQDGMGYMLAEIERGADAVGGIPSTALKGAGLLPHI
                     RATVKLPMIVMKRTLQQLLGGAPFIISGACGMFRTDVLRKFGFSDRTKVEDLDLTWTL
                     VANGYRIRQANRCIVYPQECNSPREEWRRWRRWIVGYAVCMRLHKRLLFSRFGIFSIF
                     PMLLVVLYGVGIYLTTWFNEFITTGPHGVVLAMFPLIWVGVVCVIGAFSAWFHRCWLL
                     VPLAPLSVVYVLLAYAIWIIYGLIAFFTGREPQRDKPTRYSALVEASTAYSHPSVTGT
                     EKLSEA"
     gene            1539..2063
                     /locus_tag="ACHKBL_001577"
     CDS             1539..2063
                     /locus_tag="ACHKBL_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020231914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFO5818461.1"
                     /translation="MIIDESAGEVVIGANTRICHGAVIQGPVVIGANCLIGNYAFIRP
                     GTIISNGVKIGFATEIKNAVIEAEATIGPQCFIADSVVANQAYLGAQVRTSNHRLDEQ
                     PVSVRTPEGIIATGCDKLGCYIGQRSRLGVQVIILPGRIISPNTQLGPRVIVERNLPT
                     GTYSLRQELIRTGD"
     gene            2065..2838
                     /gene="yaiO"
                     /locus_tag="ACHKBL_001578"
     CDS             2065..2838
                     /gene="yaiO"
                     /locus_tag="ACHKBL_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiO family outer membrane beta-barrel protein"
                     /protein_id="HFO5818462.1"
                     /translation="MIKRTLLAAAIFSTLPAYAGLTSITAGYDFTDYSGDHGNRNLAY
                     AELVAKVENATLLFNLSQGRRDYETEHFNATRGQGAVWYKWNNWLTTRTGIAFADNTP
                     VFARQDFRQDINLALLPKTLFTTGYRYTKYYDDVEVDAWQGGVSLYTGPVITSYRYTH
                     YDSSDAGGSYSNMISVRLNDPRGTGYTQLWLSRGTGAYTYDWTPETRYGSMKSVSLQR
                     IQPLTEQLNLGLTAGKVWYDTPTDDYNGLQLAAHLTWKF"
     gene            3023..3298
                     /gene="frmR"
                     /locus_tag="ACHKBL_001579"
     CDS             3023..3298
                     /gene="frmR"
                     /locus_tag="ACHKBL_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formaldehyde-responsive transcriptional
                     repressor FrmR"
                     /protein_id="HFO5818463.1"
                     /translation="MPSTPEEKKKVLTRVRRIRGQIDALERSLEGDAECRAILQQIAA
                     VRGAANGLMAEVLESHIRETFDRNDCYSREVSQSVDDTIELVRAYLK"
     gene            3333..4442
                     /gene="frmA"
                     /locus_tag="ACHKBL_001580"
     CDS             3333..4442
                     /gene="frmA"
                     /locus_tag="ACHKBL_001580"
                     /EC_number="1.1.1.284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase"
                     /protein_id="HFO5818464.1"
                     /translation="MKSRAAVAFAPGKPLEIVEIDVAPPKKGEVLIKVTHTGVCHTDA
                     FTLSGDDPEGVFPVVLGHEGAGVVVEVGEGVTSVKPGDHVIPLYTAECGECEFCRSGK
                     TNLCVAVRETQGKGLMPDGTTRFSYNGQPLYHYMGCSTFSEYTVVAEVSLAKINPEAN
                     HEHVCLLGCGVTTGIGAVHNTAKVQPGDSVAVFGLGAIGLAVVQGARQAKAGRIIAID
                     TNPKKFDLARRFGATDCINPNDYDKPIKDVLLDINKWGIDHTFECIGNVNVMRAALES
                     AHRGWGQSVIIGVAGSGQEISTRPFQLVTGRVWKGSAFGGVKGRSQLPGMVEDAMKGD
                     IDLEPFVTHTMSLDEINDAFDLMHEGKSIRTVIRY"
     gene            4534..5367
                     /gene="fghA"
                     /locus_tag="ACHKBL_001581"
     CDS             4534..5367
                     /gene="fghA"
                     /locus_tag="ACHKBL_001581"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018738 - S-formylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0046294 - formaldehyde catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HFO5818465.1"
                     /translation="MELIEKHASFGGWQNVYRHYSPSLKCEMNVGVYLPPKAANEKLP
                     VLYWLSGLTCNEQNFITKSGMQRYAAEHNIIVVAPDTSPRGSHVADADRYDLGQGAGF
                     YLNATQAPWNEHYKMYDYIRNELPDLVMQHFPATTRKSISGHSMGGLGALVLALRNPD
                     EYVSVSAFAPIVSPSQVPWGQQAFTAYLGENKDAWLDYDPVSLISQGQRVAEIMVDQG
                     LSDDFYAEQLRTPNLEKICQEMNIKTLIRYHEGYDHSYYFVSSFIGEHIAYHANKLNM
                     R"
     gene            complement(5594..6133)
                     /gene="yaiL"
                     /locus_tag="ACHKBL_001582"
     CDS             complement(5594..6133)
                     /gene="yaiL"
                     /locus_tag="ACHKBL_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2058 domain-containing protein"
                     /protein_id="HFO5818466.1"
                     /translation="MAKLTLQEQLLKAGLVTSKKAAKVERTAKKSRVQAREARAAVEE
                     NKKAQLERDKQLSEQQKQAALAKEYKAQVKQLIEMNRITIANGDIGFNFTDGNLIKKI
                     FVDKLTQAQLINGRLAIARLLVDNNSEGEYAIIPASVADKIAQRDASSIVLHSALSAE
                     EQDEDDPYADFKVPDYLMW"
     gene            complement(6235..7446)
                     /gene="mhpT"
                     /locus_tag="ACHKBL_001583"
     CDS             complement(6235..7446)
                     /gene="mhpT"
                     /locus_tag="ACHKBL_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-(3-hydroxy-phenyl)propionate transporter MhpT"
                     /protein_id="HFO5818467.1"
                     /translation="MSTRTPSSSSTRLMLTIGLCFLVALMEGLDLQAAGIAAGGIAQA
                     FALDKMQMGWIFSAGILGLLPGALVGGMLADRYGRKRILIGSVALFGLFSLATAIAWD
                     FPSLVFARLMTGVGLGAALPNLIALTSEAAGPRFRGTAVSLMYCGVPIGAALAATLGF
                     AGANLAWQTVFWVGGVVPLILVPLLMRWLPESAVFAGEKQAAPPLRALFAPETATATL
                     LLWLCYFFTLLVVYMLINWLPLLLVEQGFQPSQAAGVMFALQMGAASGTLMLGALMDK
                     LRPVTMSLLIYSGMLASLLALGTVSSFNGMLLAGFVAGLFATGGQSVLYALAPLFYSS
                     QIRATGVGTAVAVGRLGAMSGPLLAGKMLALGIGTVGVMAASAPGILVAGLAVFILMS
                     RRSRMQPCADA"
     gene            complement(7622..8635)
                     /gene="mhpE"
                     /locus_tag="ACHKBL_001584"
     CDS             complement(7622..8635)
                     /gene="mhpE"
                     /locus_tag="ACHKBL_001584"
                     /EC_number="4.1.3.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxovalerate aldolase"
                     /protein_id="HFO5818468.1"
                     /translation="MNGKKLYISDVTLRDGMHAIRHQYSLENVRQVAKALDDARVDSI
                     EVAHGDGLQGSSFNYGFGAHSDLEWIEAAADVVKHAKIATLLLPGIGTIHDLKNAWQA
                     GARVVRVATHCTEADVSAQHIQYARELGMDTVGFLMMSHMTTPENLAKQAKLMEGYGA
                     TCIYVVDSGGAMNMSDIRDRFRALKAVLKPETQTGIHAHHNLSLGVANSIAAVEEGCD
                     RIDASLAGMGAGAGNAPLEVFIAAADKLGWQHGTDLYALMDAADDLVRPLQDRPVRVD
                     RETLALGYAGVYSSFLRHCETAAARYGLSAVDILVELGKRRMVGGQEDMIVDVALDLR
                     NNK"
     gene            complement(8632..9582)
                     /gene="mhpF"
                     /locus_tag="ACHKBL_001585"
     CDS             complement(8632..9582)
                     /gene="mhpF"
                     /locus_tag="ACHKBL_001585"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetaldehyde dehydrogenase"
                     /protein_id="HFO5818469.1"
                     /translation="MSKRKVAIIGSGNIGTDLMIKILRHGQHLEMAVMVGIDPQSDGL
                     ARARRMGVATTHEGVIGLMNMPEFADIDIVFDATSAGAHVKNDAALREAKPDIRLIDL
                     TPAAIGPYCVPVVNLEENVAQLNVNMVTCGGQATIPMVAAVSRVARVHYAEIIASIAS
                     KSAGPGTRANIDEFTETTSRAIEVVGGAAKGKAIIVLNPAEPPLMMRDTVYVLSDEAS
                     QDDIEASINEMAEAVQAYVPGYRLKQRVQFEVIPQDKPVNLPGVGQFSGLKTAVWLEV
                     EGAAHYLPAYAGNLDIMTSSALATAEKMAQSLARKAGEAA"
     gene            complement(9579..10388)
                     /gene="mhpD"
                     /locus_tag="ACHKBL_001586"
     CDS             complement(9579..10388)
                     /gene="mhpD"
                     /locus_tag="ACHKBL_001586"
                     /EC_number="4.2.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-4-pentenoate hydratase"
                     /protein_id="HFO5818470.1"
                     /translation="MTKHTLEQLAADLRRAAEQGEAIAPLRDLIGIDNAEAAYAIQHI
                     NVQHDVAQGRRVVGRKVGLTHPKVQQQLGVDQPDFGTLFADMCYGDNETIPFSRVLQP
                     RIEAEIALVLNRDLPATDITFDELYNAIEWVLPALEVVGSRIRDWSIQFVDTVADNAS
                     CGVYVIGGPAQRPAGLDLKNCAMKMTRNNEEVSSGRGSECLGHPLNAAVWLARKMASL
                     GEPLRAGDIILTGALGPMVAVNAGDRFEAHIEGIGSVAATFSSAAPKGSLS"
     gene            complement(10398..11264)
                     /gene="mhpC"
                     /locus_tag="ACHKBL_001587"
     CDS             complement(10398..11264)
                     /gene="mhpC"
                     /locus_tag="ACHKBL_001587"
                     /EC_number="3.7.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414883.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-6-oxonona-2,4-dienedioate hydrolase"
                     /protein_id="HFO5818471.1"
                     /translation="MSYQPQTEAATSRFLNVEEAGKTLRIHFNDCGQGDETVVLLHGS
                     GPGATGWANFSRNIDPLVKAGYRVILLDCPGWGKSDSIVNSGSRSDLNARILKSVVDQ
                     LDIAKIHLLGNSMGGHSSVAFTLNWPERVGKLVLMGGGTGGMSLFTPMPTEGIKRLNQ
                     LYRQPTIENLKLMMDIFVFDTSDLTDALFEARLNNMLSRRDHLENFVKSLEANPKQFP
                     DFGPRLAEIKAQTLIVWGRNDRFVPMDAGLRLLSGIAGSELHIFRDCGHWAQWEHADA
                     FNQLVLNFLARP"
     gene            complement(11282..12226)
                     /gene="mhpB"
                     /locus_tag="ACHKBL_001588"
     CDS             complement(11282..12226)
                     /gene="mhpB"
                     /locus_tag="ACHKBL_001588"
                     /EC_number="1.13.11.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydroxyphenylpropionate/2,
                     3-dihydroxicinnamic acid 1,2-dioxygenase"
                     /protein_id="HFO5818472.1"
                     /translation="MHAYLHCLSHSPLVGYVDPAQEVLDEVNGVIASARERIAAFSPE
                     LVVLFAPDHYNGFFYDVMPPFCLGVGATAIGDFGSAAGELPVPVELAEACAHAVMKSG
                     IDLAVSYCMQVDHGFAQPLEFLLGGLDKVPVLPVFINGVATPLPGFQRTRMLGEAIGR
                     FTSTLNKRVLFLGSGGLSHQPPVPELAKADAHMRDRLLGSGKDLPASERELRQQRVIS
                     AAEKFVEDQRTLHPLNPIWDNQFMTLLEQGRIQELDAVSNEELSAIAGKSTHEIKTWV
                     AAFAAISAFGNWRSEGRYYRPIPEWIAGFGSLSARTEN"
     gene            complement(12228..13892)
                     /gene="mhpA"
                     /locus_tag="ACHKBL_001589"
     CDS             complement(12228..13892)
                     /gene="mhpA"
                     /locus_tag="ACHKBL_001589"
                     /EC_number="1.14.13.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008688 - 3-(3-hydroxyphenyl)propionate
                     hydroxylase activity [Evidence IEA];
                     GO_process: GO:0019622 - 3-(3-hydroxy)phenylpropionate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3-(3-hydroxy-phenyl)propionate/3-hydroxycinnamic acid
                     hydroxylase"
                     /protein_id="HFO5818473.1"
                     /translation="MAIQHPDIQPAVNHSVQVAIAGAGPVGLMMANYLGQMGIDVLVV
                     EKLDKLIDYPRAIGIDDEALRTMQSVGLVDNVLPHTTPWHAMRFLTPKGRCFADIQPM
                     TDEFGWPRRNAFIQPQVDAVMLEGLSRFPNVRCLFSRELEAFSQQDDEVTLHLKTAEG
                     QRETVKAQWLVACDGGASFVRRTLNVPFEGKTAPNQWIVVDIANDPLSTPHIYLCCDP
                     VRPYVSAALPHAVRRFEFMVMPGETEEQLREPQNMRKLLSKVLPNPDNVELIRQRVYT
                     HNARLAQRFRIDRVLLAGDAAHIMPVWQGQGYNSGMRDAFNLAWKLALVIQGKARDAL
                     LDTYQQERRDHAKAMIDLSVTAGNVLAPPKRWQGTLRDGVSWLLNYLPPVKRYFLEMR
                     FKPMPQYYGGALVREGEAKHSPVGKMFIQPKVTLENGDVTLLDNAIGANFAVIGWGCN
                     PLWGMSDEQIQQWRALGTRFIQVVPEVQIHTAQDNHDGVLRVGDTQGRLRSWFAQHNA
                     SLVVMRPDRFVAATAIPQTLGNTLNKLASVMTLTRPDADVSVEKVA"
     gene            14083..14916
                     /gene="mhpR"
                     /locus_tag="ACHKBL_001590"
     CDS             14083..14916
                     /gene="mhpR"
                     /locus_tag="ACHKBL_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HFO5818474.1"
                     /translation="MQNNEQTAYKTVRGLTRGLMLLNMLNKLDGGASVGLLAELSGLH
                     RTTVRRLLETLQEEGYVRRSPSDDSFRLTIKVRQLSEGFRDEQWISALAAPLLGDLLR
                     EVVWPTDVSTLDVDAMVVRETTHRFSRLSFHRAMVGRRLPLLKTASGLTWLAFCPEQE
                     RKELIEMLASRPGDDYQLAREPLKLEAILARARKEGYGQNYRGWDQEEKIASIAVPLR
                     SEQRVIGCLNLVYMASAMTIEQAAEKHLPALQRVAKQIEEGVESQAILVAGRRSGVHL
                     R"
     gene            14993..16075
                     /gene="lacI"
                     /locus_tag="ACHKBL_001591"
     CDS             14993..16075
                     /gene="lacI"
                     /locus_tag="ACHKBL_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414879.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor LacI"
                     /protein_id="HFO5818475.1"
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQACHVSAKTREKVEAAMAE
                     LNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERS
                     GVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACANVPALFLDVSDQTPINSII
                     FSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREG
                     DWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDT
                     EDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLPPNT
                     QTASPQALADSLMQLARQISRLESGQ"
     gene            16198..19272
                     /gene="lacZ"
                     /locus_tag="ACHKBL_001592"
     CDS             16198..19272
                     /gene="lacZ"
                     /locus_tag="ACHKBL_001592"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HFO5818476.1"
                     /translation="MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSKEAR
                     TDRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPDADTVVVPSNWQMHGYDAPIYT
                     NVTYPITVNPPFVPAENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWV
                     GYGQDSRLPSEFDLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDISLLHK
                     PTTQISDFHVTTRFNDDFSRAVLEAEVQVYGELRDELRVTVSLWQGETQVASGTTPFG
                     GEIIDERGGYADRVTLRLNVENPALWSAEIPNIYRAVVELHTADGTLIEAEACDVGFR
                     EVRIENGLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSH
                     YPNHPLWYTLCDRYGLYVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVLRDRN
                     HPSVIIWSLGNESGHGANHDALYRWIKSVDPSRPVQYEGGGADTSATDIICPMYARVD
                     EDQPFPAVPKWSIKKWLSLPGELRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGG
                     FVWDWVDQSLIKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQ
                     QQFFQFRLSGRTIEVTSEYLFRHSDNELLHWSVALDGKPLASGEMPLDVAPQDKQLIE
                     LPELPQPESAGQLWLTVHVVQPNATAWSEAGHISAWQQWRLTENLSVTLPSAPHAIPQ
                     LTTSETDFCIELGNKRWQFNRQSGFLSQMWIGDEKQLLTPLRDQFTRAPLDNDIGVSE
                     ATRIDPNAWVERWKAAGHYQTEVALLQCTADTLADAVLITTAHAWQHQGKTLFISRKT
                     YRIDGSGQMAITVDVEVASDTPHPARIGLTCQLAQVAERVNWLGLGPQENYPDRLTAA
                     CFDRWDLPLSDMYTPYVFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQQQLMETS
                     HRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAEFQLSAGRYHYQLVWCQK"
     gene            19324..20577
                     /gene="lacY"
                     /locus_tag="ACHKBL_001593"
     CDS             19324..20577
                     /gene="lacY"
                     /locus_tag="ACHKBL_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactose permease"
                     /protein_id="HFO5818477.1"
                     /translation="MYYLKNTNFWMFGLFFFFYFFIMGAYFPFFPIWLHDINHISKSD
                     TGIIFAAISLFSLLFQPLFGLLSDKLGLRKYLLWIITGMLVMFAPFFIFIFGPLLQYN
                     ILVGSIVGGIYLGFCFNAGAPAVEAFIEKVSRRSNFEFGRARMFGCVGWALCASIVGI
                     MFTINNQFVFWLGSGCALILAVLLFFAKTDAPSSATVANAVGANHSAFSLKLALELFR
                     QPKLWFLSLYVIGVSCTYDVFDQQFANFFTSFFATGEQGTRVFGYVTTMGELLNASIM
                     FFAPLIINRIGGKNALLLAGTIMSVRIIGSSFATSALEVVILKTLHMFEVPFLLVGCF
                     KYITSQFEVRFSATIYLVCFCFFKQLAMIFMSVLAGNMYESIGFQGAYLVLGLVALGF
                     TLISVFTLSGPGPLSLLRRQVNEVA"
     gene            20643..21254
                     /gene="lacA"
                     /locus_tag="ACHKBL_001594"
     CDS             20643..21254
                     /gene="lacA"
                     /locus_tag="ACHKBL_001594"
                     /EC_number="2.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactoside O-acetyltransferase"
                     /protein_id="HFO5818478.1"
                     /translation="MNMPMTERIKAGKLFTDMCEGLPEKRLRGKTLMYEFNHSHPSEV
                     EKRESLIKEMFATVGENAWVEPPVHFSYGSNIHIGRNFYANFNLTIVDDYTVTIGDNV
                     LIAPNVTLSVTGHPVHHELRKNGEMYSFPITIGNNVWIGSHVVINPGVTIGDNSVIGA
                     GSVVIKDIPPNVVAAGVPCRVIREINDRDKHYYYKDYKVESSV"
     gene            complement(21357..22538)
                     /gene="cynX"
                     /locus_tag="ACHKBL_001595"
     CDS             complement(21357..22538)
                     /gene="cynX"
                     /locus_tag="ACHKBL_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyanate transporter CynX"
                     /protein_id="HFO5818479.1"
                     /translation="MRNHQKTFTMLLVLVLIGLNMRPLLTSVGPLLPQLRQASGMSFS
                     VAALLTALPVVTMGGLALAGSWLHQHVSERRSVAISLLLIAVGALMRELYPQSALLLS
                     SALLGGVGIGIIQAVMPSVIKRRFQHRTPLVMGLWSAALMGGGGLGAAITPWLVQHSE
                     TWYQTLAWWALPAVVALFAWWWQNAREVASSHKTTTTPVRVVFTPRAWTLGVYFGLIN
                     GGYASLIAWLPAFYIEIGASAQYSGSLLALMTLGQAAGALLMPAMARHQDRRKLLMLA
                     LALQLVGFCGFIWLPLQLPVFWAMVCGLGLGGAFPLCLLLALDHSAQPAIAGKLVAFM
                     QGIGFIIAGLAPWFSGVLRSISGNYLMDWAFHALCVVGLMIITLRFAPARFPQLWVKE
                     A"
     gene            complement(22544..23014)
                     /gene="cynS"
                     /locus_tag="ACHKBL_001596"
     CDS             complement(22544..23014)
                     /gene="cynS"
                     /locus_tag="ACHKBL_001596"
                     /EC_number="4.2.1.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008824 - cyanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009439 - cyanate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanase"
                     /protein_id="HFO5818480.1"
                     /translation="MIQSQINRNIRLDLADAILLSKAKKDLSFAEIADGTGLAEAFVT
                     AALLGQQALPADAARLVGAKLDLDEDAILLLQMIPLRGCIDDRIPTDPTMYRFYEMLQ
                     VYGTTLKALVHEKFGDGIISAINFKLDVKKVADPEGGERAVITLDGKYLPTKPF"
     gene            complement(23045..23704)
                     /gene="cynT"
                     /locus_tag="ACHKBL_001597"
     CDS             complement(23045..23704)
                     /gene="cynT"
                     /locus_tag="ACHKBL_001597"
                     /EC_number="4.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase CynT"
                     /protein_id="HFO5818481.1"
                     /translation="MKEIIDGFLKFQREAFPKREALFKQLATQQSPRTLFISCSDSRL
                     VPELVTQREPGDLFVIRNAGNIVPSYGPEPGGVSASVEYAVAALRVSDIVICGHSNCG
                     AMTSIASCQCMDHMPAVSHWLRYADSARVVNEARPHPDLPSKAAAMVRENVIAQLANL
                     QTHPSVRLALEEGRIALHGWVYDIESGSIAAFDGATRQFVPLAANPRVCAIPLRQPTA
                     A"
     gene            23814..24713
                     /gene="cynR"
                     /locus_tag="ACHKBL_001598"
     CDS             23814..24713
                     /gene="cynR"
                     /locus_tag="ACHKBL_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414872.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator CynR"
                     /protein_id="HFO5818482.1"
                     /translation="MLSRHINYFLAVAEHGSFTRAASALHVSQPALSQQIRQLEESLG
                     VPLFDRSGRTIRLTDAGEVWRQYASRALQELGAGKRAIHDVADLTRGSLRIAVTPTFT
                     SYFIGPLMADFYARYPGITLQLQEMSQEKIEDLLCRDELDVGIAFAPVHSPELEAIPL
                     LTESLALVVAQHHPLAACEQVALSRLHDEKLVLLSAEFATREQIDHYCEKAGLHPQVV
                     IEANSISAVLELIRRTSLSTLLPAAIATQHDGLKAISLAPPLLERTAVLLRRKNSWQT
                     AAAKAFLHMALDKCAVVGGNESR"
     gene            complement(24715..24806)
                     /locus_tag="ACHKBL_001599"
                     /pseudo
     CDS             complement(24715..24806)
                     /locus_tag="ACHKBL_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(24846..26129)
                     /gene="codA"
                     /locus_tag="ACHKBL_001600"
     CDS             complement(24846..26129)
                     /gene="codA"
                     /locus_tag="ACHKBL_001600"
                     /EC_number="3.5.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine/isoguanine deaminase"
                     /protein_id="HFO5818483.1"
                     /translation="MSNNALQTIINARLPGKEGLWQIHLLDGKISAIDAQSGVMPITE
                     NSLDAEQGLVIPPFVEPHIHLDTTQTAGQPNWNQSGTLFEGIERWAERKALLTHDDVK
                     QRAWQTLKWQIANGIQHVRTHVDVSDATLTALKAMLEVKQEVAPWIDLQIVAFPQEGI
                     LSYPNGEALLEEALRLGADVVGAIPHFEFTREYGVESLHKTFALAQKYDRLIDVHCDE
                     IDDEQSRFVETVAALAHREGMGARVTASHTTAMHSYNGAYTSRLFRLLKMSGINFVAN
                     PLVNIHLQGRFDTYPKRRGITRVKEMLESGINVCFGHDDVFDPWYPLGTANMLQVLHM
                     GLHVCQLMGYGQINDGLNLITHHSARTLNLQDYGIAAGNSANLIILPAENGFDALRRQ
                     VPVRYSVRGGKVIASTQPAQTTVYLEQPEAIDYKR"
     gene            complement(26119..27378)
                     /gene="codB"
                     /locus_tag="ACHKBL_001601"
     CDS             complement(26119..27378)
                     /gene="codB"
                     /locus_tag="ACHKBL_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015209 - cytosine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HFO5818484.1"
                     /translation="MSQDNNFSQGPVPQSARKGVLALTFVMLGLTFFSASMWTGGTLG
                     TGLSYHDFFLAVLIGNLLLGIYTSFLGYIGAKTGLTTHLLARFSFGVKGSWLPSLLLG
                     GTQVGWFGVGVAMFAIPVGKATGLDINLLIAVSGLLMTVTVFFGISALTVLSVIAVPA
                     IACLGGYSVWLAVNGMGGLDALKAVVPAQPLDFNVALALVVGSFISAGTLTADFVRFG
                     RNAKLAVLVAMVAFFLGNSLMFIFGAAGAAALGMADISDVMIAQGLLLPAIVVLGLNI
                     WTTNDNALYASGLGFANITGMSSKTLSVINGIIGTVCALWLYNNFVGWLTFLSAAIPP
                     VGGVIIADYLMNRRRYEHFATTRMMSVNWVAILAVALGIAAGHWLPGIVPVNAVLGGA
                     LSYLILNPILNRKTTAAMTHVEANSVE"
BASE COUNT         6541 a         7536 c         7203 g         6294 t
ORIGIN      
        1 tgaccgccat caggatcatc ttgcagtcta tcaagcctca atggttgcct gtcgcgctat
       61 tccgcaaatt ctcggctatg aaacaccaag tacctggctt tcatttatgc ctcaggtttt
      121 tgaatccgtg aaagaagaat atttttctct taaactgact gcgttaaaaa aacataaaag
      181 tcagagccag cgagattata tgcgcccgga gcgtctgcgt gcagttgctc aatttcgcgg
      241 gcaacaggtc aatagcgatc tgggtgaagg ttttgttatt cataaaatga ttctttgagc
      301 aaattgcatg aaaacctgga tctttatatg tatggccgta gcaatattgc tatggtttct
      361 gagtacgtta aggcgtaagc ccagccaaaa gaaaggctgt attgacgcca ttatacctgc
      421 ttataacgaa ggcccgtgtc tggcgcagtc actggataat ctgctgcgta acccttattt
      481 ctgccgggta atttgcgtta acgacggctc cacggacaat accgaagcgg tcatggcgga
      541 agtcaaacgc aaatggggcg accgctttat tgccgtcacg caaaaaaata ccggtaaagg
      601 tggtgcgcta atgaatggcc tcaattacgc cacctgcgat caggtttttt taagtgatgc
      661 cgacacctat gttccgcccg atcaagacgg aatgggctat atgctggcag aaatagagcg
      721 cggtgctgat gccgtaggcg gcattccctc tactgcgttg aaaggcgcgg gtctgttacc
      781 gcacatccgc gcgaccgtaa agttgccgat gattgttatg aagcgcacgc tacagcagct
      841 cctgggcggc gcaccgttta ttatcagcgg tgcctgcggg atgttccgta ctgatgtatt
      901 gcgtaagttc ggtttctctg atcgtactaa agtcgaagac cttgatctca cctggacatt
      961 ggtggcaaac ggctatcgta ttcggcaggc gaatcgctgc atcgtatacc cacaggaatg
     1021 caacagcccg cgtgaggaat ggcgtcgctg gcggcgttgg attgtgggct acgcggtctg
     1081 tatgcgcctg cataaaagac ttttatttag ccgcttcggt atcttcagta tatttcctat
     1141 gctgttggtt gtgctttatg gcgttgggat ttatctcact acctggttta atgaattcat
     1201 caccaccggg ccacatggtg tggtgttggc aatgttcccg cttatctggg tcggcgtagt
     1261 ttgtgttatt ggtgcattta gcgcctggtt tcatcgttgc tggttgttgg tgcctttagc
     1321 accgctttcc gttgtgtatg tattattagc ttatgccatc tggattattt atggacttat
     1381 tgcctttttt actggacgcg aacctcagcg cgacaaaccc acccgctatt ccgcactggt
     1441 ggaagcgtca accgcttatt cccacccttc tgtcacagga actgaaaaac tatctgaagc
     1501 ttaatgttaa agagaaaaat attcagattg cagaccaggt gattattgat gaaagtgccg
     1561 gtgaggttgt tatcggcgcg aatacccgta tttgccacgg tgccgttatt cagggtccgg
     1621 tagtgattgg cgcaaactgc ctgataggta attatgcgtt tattcgtcct ggcacaataa
     1681 tcagcaatgg cgtaaaaatt ggttttgcca ccgaaattaa aaatgcggtt attgaagcgg
     1741 aagcaacgat tggtccgcaa tgctttattg ccgactcggt agttgcaaac caggcatatt
     1801 tgggcgcaca agtacgaacc agtaatcatc gtctggatga acagcccgtg tctgttcgaa
     1861 ctccagaggg aattatcgct accggatgcg ataaattagg ttgttatatc gggcagcgtt
     1921 cacgccttgg cgtacaagtt attattttgc ctgggcgaat tatttccccg aacacgcaac
     1981 ttggcccgcg cgtgattgta gaacgtaatt tacctaccgg aacttattca ctccgacaag
     2041 aacttatccg tacaggagat taaaatgata aaacggacgt tattagcggc ggcaatattt
     2101 agcacattgc ccgcttatgc cgggttaact tccattaccg cgggctacga ttttaccgat
     2161 tattctggcg atcatggcaa ccgtaattta gcgtatgctg aactggtggc gaaagttgaa
     2221 aacgcaacgc tgctttttaa tctttcccag gggcgtcgtg attatgaaac tgaacatttc
     2281 aatgccactc gcggtcaggg tgcggtctgg tataaatgga ataactggct gacaacccga
     2341 acgggtattg cctttgcgga taatacgccg gtctttgccc gccaggattt tcgtcaggat
     2401 attaacctgg ccctgttgcc aaaaacgctt ttcacgaccg gttatcgcta cactaaatat
     2461 tacgatgatg tcgaagtcga tgcctggcaa ggcggcgtat ccctctatac aggcccggtg
     2521 atcaccagct atcgctatac ccattatgac tccagcgatg caggtggtag ttacagcaat
     2581 atgatttccg tgcgtctgaa tgacccgcgc ggcactggtt atacgcaact atggctaagc
     2641 cgcggaacag gtgcttacac ctatgactgg acgccagaaa cacgctacgg cagcatgaag
     2701 agcgtcagtt tgcaacgtat tcaaccgctg actgagcaac ttaatctcgg gctgacggca
     2761 ggtaaagtgt ggtacgacac cccaaccgat gattataacg gtctgcaact tgcagcccat
     2821 ctgacctgga aattctgatt ccttctgccg cccgctatcc ggggcggcct tccctgtcga
     2881 ttaggcccct ctttactttt tgttatccga ctacattcac cggataaatt ttattctcca
     2941 gtgttatata ctatagaggg gtatgcattg acatataaca taccccccta tagtatattg
     3001 cgtgcagatg atgaggtgcg aaatgcccag tactccggaa gagaagaaaa aggtccttac
     3061 tcgagttcgt cgtattcggg ggcagattga tgctctggaa cggtcgctgg agggtgatgc
     3121 cgaatgccgt gccatactcc aacagatcgc tgccgttcgg ggcgcggcta acggactgat
     3181 ggcagaagtg cttgaaagcc atatccggga aacgtttgac cgaaatgact gctacagccg
     3241 cgaagtcagc caatccgttg acgacactat tgaactggtt cgtgcctatc ttaaatagct
     3301 gaatctatta ccatattgag gaagagcgag agatgaaatc acgtgctgcc gttgcatttg
     3361 ctcccggtaa accgctggaa atcgttgaaa ttgacgttgc accaccgaaa aaaggtgaag
     3421 tgctgattaa agtcacccat accggtgttt gccataccga cgcatttacc ctctccggcg
     3481 atgacccgga aggtgtcttc ccggtggttc tcggtcacga aggggccggg gttgtagttg
     3541 aagtcggtga aggcgtaacc agcgtcaaac ctggcgacca tgtgatcccg ctttacaccg
     3601 cagagtgcgg cgagtgtgag ttctgtcgtt ctggcaaaac caacctgtgt gtcgcggttc
     3661 gcgaaacgca gggcaaaggc ttgatgccag acggcaccac ccgtttttct tacaacgggc
     3721 agccgcttta tcactacatg ggatgctcaa cattcagtga atacaccgtg gtcgcggaag
     3781 tgtctctggc caaaattaat ccagaagcaa accatgaaca cgtctgcctg ctgggctgtg
     3841 gcgtgaccac cggtattggc gcggtacaca acacagctaa agtccagcca ggtgattctg
     3901 ttgccgtgtt tggtcttggc gcgattggtc tggcagtggt tcagggcgcg cgtcaggcga
     3961 aagcgggacg gattatcgct atcgatacca acccgaagaa attcgatctg gcgcgtcgct
     4021 tcggtgccac cgactgcatt aacccgaatg actacgacaa accgattaaa gatgtcctgc
     4081 tggatatcaa caaatggggt atcgaccata cctttgaatg catcggtaac gtcaacgtga
     4141 tgcgtgcggc gctggaaagt gcgcaccgcg gctggggtca gtcggtgatc atcggggtag
     4201 ctggttctgg tcaggaaatc tccacccgtc cattccagtt ggtcaccggt cgcgtatgga
     4261 aaggttccgc gtttggcggc gtgaaaggtc gttcccagtt accgggtatg gttgaagatg
     4321 cgatgaaagg cgatatcgat ctggaaccgt ttgtcacgca taccatgagc ctggatgaaa
     4381 ttaatgacgc ctttgacctg atgcatgaag gcaaatccat tcgaaccgta attcgttact
     4441 gatttcccgc aggtttaccc gtccagttca gacggggttc ttaatactct ccccgggcag
     4501 ccgtccgggg gattaaccgt gagataatga ctgatggaac tcattgaaaa acatgccagc
     4561 tttggcggct ggcaaaatgt gtatcggcat tattccccat cactgaaatg tgaaatgaat
     4621 gtcggcgtct atctcccgcc aaaagccgca aatgaaaaat tgccggtgtt gtactggctt
     4681 tcaggcctga cctgcaacga gcagaatttc attactaaat cggggatgca gcgttacgcg
     4741 gctgagcaca acattattgt tgttgcgcca gacaccagtc cgcgaggcag tcatgtcgca
     4801 gatgccgacc gttacgatct cgggcaaggt gccgggtttt acctgaacgc aacgcaagcg
     4861 ccgtggaatg aacattacaa aatgtatgac tatatccgca acgagctgcc ggatttagtg
     4921 atgcaacatt ttccggcaac gaccagaaag tctatctcag gtcattctat gggcgggctg
     4981 ggcgcgctgg tgctggcgtt acgtaaccca gatgaatatg tcagcgtctc ggcgtttgcg
     5041 cccattgtct ccccatcgca agtaccgtgg ggacagcaag cctttaccgc atatcttggt
     5101 gaaaataaag atgcctggtt ggattacgat ccggtgagtc ttatttcaca aggtcaacgc
     5161 gttgcggaaa taatggttga tcaggggttg agtgatgatt tttacgcaga acagctgcgg
     5221 actccaaatc ttgaaaagat ctgccaggag atgaatatca aaacgttaat ccgttatcac
     5281 gagggttatg atcacagcta ttattttgtc tccagtttta ttggcgagca tattgcgtac
     5341 cacgccaata aactgaatat gcgttgagca tccgcgcacg catgccggat gcggcgtaaa
     5401 cgccttatcc ggcctacatt tcacccgtaa gccgtaggcc tgataagacg cgaaaagcgt
     5461 cgcatcaggc atctgcgcac ggctgccgga tgcggcgtga acgccttatc cggcctacac
     5521 ttcgcccgta aaccgtaggc ctgataagac gcgtaagcgt cgcatcaggc atcactgcac
     5581 ggcttatcta tgtttaccac atcaaataat caggcacttt gaagtcggca tacggatcgt
     5641 cttcatcctg ctcttcggcg ctgagcgcgc tgtgcaacac aatactgctg gcatcacgct
     5701 gagcaatttt atcggcaaca ctggcgggaa taatggcgta ttcaccttcg ctattgttat
     5761 cgaccaacaa gcgggcaatc gccagacgac cattaatcaa ctgcgcctgt gtgagcttat
     5821 cgacaaaaat cttcttaatc agattgccgt cagtgaagtt aaaaccaata tcgccattgg
     5881 caatggtgat tcggttcatt tcaatgagct gcttcacctg ggctttatat tctttcgcca
     5941 acgccgcttg tttttgctgt tcgctaagct gtttatcacg ctcaagctgt gcctttttat
     6001 tttcttctac cgccgcccga gcttcacgcg cctgaacgcg cgattttttc gccgttctct
     6061 cgaccttcgc cgcttttttg ctggtgacta atcctgcttt gagcaactgc tcttgtaagg
     6121 taagttttgc catctttgtt tccaaacccg ctgaataatt tttagcgagt atacctgtaa
     6181 acgccgtagg tgtattgggg gcaagtccct ttcgcccctg cttaataagg cgcgtcaggc
     6241 atcggcgcac ggctgcattc gtgatctccg gctcatcaaa ataaatactg ccaggccagc
     6301 aacaagaata cccggcgcag aagcggccat tacgccaacc gtgccaatgc ctaatgccag
     6361 cattttcccg gccagtaacg gaccgctcat agcccccaga cgccctaccg ccacggctgt
     6421 tcccacacct gttgcgcgga tctgcgaact gtaaaacaac ggtgccaggg catacaaaac
     6481 gctttgccca cctgtcgcaa acaaccccgc gacgaatccc gccagcaaca taccgttaaa
     6541 tgacgacacc gttcccagcg ccagcagcga agctaacatg ccgctataaa tcagtagcga
     6601 catggttact ggacgcagct tatccatcaa tgcgcccaac attaacgtcc cgcttgccgc
     6661 ccccatttgc agtgcaaaca tcacccctgc cgcctgcgat ggctggaatc cttgctccac
     6721 caaaagcagc ggtagccagt tgatcaacat gtagaccacc agcagagtga agaaataaca
     6781 caaccacagc agcagcgtcg cggttgccgt ttctggcgca aataacgcac gcagtggtgg
     6841 cgcagcctgt ttttcgccag cgaacaccgc cgactccggc agccagcgca ttaatagcgg
     6901 caccagaatc aacggcacca caccacctac ccaaaacacc gtttgccatg ctaagtttgc
     6961 ccccgcgaaa cccagtgtcg ccgccagcgc cgcgccaatg gggacaccgc aatacatcag
     7021 gctcactgcc gtcccacgaa aacgtggacc cgcggcttca gacgtcaggg cgatcagatt
     7081 cggcaacgcc gcccccagcc cgacaccggt catcagccgc gcaaagacca gtgaggggaa
     7141 atcccaggca atcgccgttg ccagtgagaa caaaccaaac agcgcaactg agccaatcaa
     7201 aatgcgcttg cgaccataac ggtccgccag cattccgcca accaacgcgc cgggtagcaa
     7261 accgagtatt ccggcgctga atatccagcc catttgcatt ttatcgagtg cgaaagcctg
     7321 ggcgatgcca cccgccgcaa tgccagccgc ctgaagatcc agtccttcca tcagagcgac
     7381 caaaaaacaa agcccgatgg tcagcatcag gcgggttgaa gatgatgaag gggtacgagt
     7441 cgacataaga tgaacctcgt tgcgagtgca ggtgaatcgc ggctgataca ttgtcagccg
     7501 cgtaatcaga accatttaat gactgcctac gggcgaaata taggccggat aaggcgttta
     7561 cgccgcatcc ggcagtcgtg ctgggatggg catacaaaat gcacgttctc ggcagtcatc
     7621 attatttgtt gttgcgcaga tccagcgcca cgtcgacgat catatcctcc tggccgccaa
     7681 ccatccggcg tttgcccagc tcaacgagaa tatccaccgc gcttaagcca taacgcgccg
     7741 ccgccgtttc acagtgacgc aggaagctcg agtaaacacc agcgtatccc agcgccagcg
     7801 tttcgcgatc gactcgtacc ggtcgatcct gcaacggacg caccaggtcg tcagcggcat
     7861 ccattaacgc atagagatcg gtcccatgct gccaacccag tttatccgcg gcggcaataa
     7921 aaacttccag cggcgcgtta cctgcgcccg cgcccattcc cgcgaggctg gcgtcgattc
     7981 ggtcgcagcc ctcttccacc gccgcgatag agttcgccac gccaagactc aggttatggt
     8041 gagcgtgtat gccggtttgc gtttctggtt tcagcactgc tttcagagcg cggaaacggt
     8101 cacggatatc gctcatgttc atcgcaccgc cagaatccac cacataaata caggtcgcac
     8161 cgtagccttc catcagcttt gcctgctttg cgagattctc cggcgtggtc atatggctca
     8221 tcatcagaaa accaacggtg tccattccga gctcgcgggc atactgaata tgctgggcgg
     8281 aaacatcagc ttcggtacag tgcgtagcca cacgaaccac ccgcgcgcca gcctgccagg
     8341 catttttcag atcgtgaata gtgccgattc caggcagcaa caacgtcgcg attttggcgt
     8401 gcttcaccac atccgccgcc gcttcaatcc attcaaggtc gctatgtgcg ccgaaaccat
     8461 agttaaagct ggaaccttgc aaaccgtcgc cgtgggccac ttcaatcgaa tccacgcggg
     8521 catcgtccag tgctttggca acctggcgaa cgttttccaa tgaatactga tgacgaatgg
     8581 cgtgcatacc gtcacgcaac gtgacgtccg agatataaag ttttttaccg ttcatgccgc
     8641 ttctcctgcc ttgcgcgcca gtgactgggc cattttttcc gctgtcgcca gcgcactgga
     8701 agtcataatg tcgaggttgc ctgcataggc aggcagataa tgcgctgcgc cttcgacttc
     8761 cagccagacc gccgttttca gtccggagaa ctgccccacg cccggcaaat taaccggttt
     8821 atcctgcggg ataacttcaa actgcacgcg ctgtttcagg cgataacccg gtacgtaagc
     8881 ctgcaccgcc tcagccattt cattgattga ggcttcgata tcatcttgtg aagcttcgtc
     8941 gctcaataca tacaccgtgt cacgcatcat caacggtggc tctgctgggt taagcacaat
     9001 aatcgccttc ccttttgccg cgccacccac cacttcaatg gcgcgcgaag tggtttccgt
     9061 aaattcatcg atattggcac gcgtgccagg tccggcagat ttactggcaa tagaagcgat
     9121 aatttcggcg taatgaacac gcgccacgcg tgaaactgcc gccaccattg gaatggtggc
     9181 ctggccgccg caggtgacca tgttgacgtt cagttgagcg acgttctcct cgaggttaac
     9241 gaccggcacg cagtaggggc cgatggcagc aggcgtcagg tcaattaagc gaatatccgg
     9301 tttcgcttcg cgcaaagcgg catcgttttt cacatgcgca cccgcgctgg tcgcatcaaa
     9361 tacaatgtcg atatcagcaa attcaggcat gttcatcagg ccgatcaccc cttcatgggt
     9421 ggtggcgacg cccatacgtc tggcacgcgc cagaccgtcg gactgaggat caatgccgac
     9481 catcaccgcc atctccagat gctggccgtg acgcaaaatt ttaatcatca gatcggtgcc
     9541 aatgttgcct gaaccgataa tggcgacttt acgcttactc atgacagact tccttttggg
     9601 gctgcgcttg aaaatgtcgc agcaactgaa cctatgcctt caatatgggc ttcaaaacga
     9661 tcgcccgcat tcaccgccac catcggacct aatgccccgg taagaatgat atctccggcg
     9721 cgcagcggtt cacccaggct ggccattttg cgtgccagcc agacggcggc attaagcgga
     9781 tgtcccaggc attcgctgcc gcgcccgctg gaaacctctt cgttattacg cgtcatcttc
     9841 atggcgcagt ttttcaggtc taaccccgcc ggacgttgcg ccggaccgcc gatgacatac
     9901 accccgcagg aagcgttatc tgccacggta tcgacaaact gaatcgacca gtcgcgaatg
     9961 cggctcccca ccacttccag cgccggaagt acccattcaa tggcgttata caattcgtcg
    10021 aaggtgatat cggttgcggg tagatcgcgg ttcaacacca gtgcaatctc cgcttcaatg
    10081 cggggttgca gaacacggga aaaaggaatg gtttcgttat cgccataaca catgtcggca
    10141 aataatgtcc cgaaatccgg ttgatcaacg cccagttgtt gctgcacttt cggatgcgtc
    10201 agccccactt tgcgccccac cactcgccgt ccctgagcga cgtcgtgttg cacattgatg
    10261 tgctgaatgg cgtaggccgc ttcagcgtta tcgataccaa tcagatcgcg cagcggcgca
    10321 atcgcttcgc cctgctctgc ggcgcgacgt aaatccgccg ccagttgctc aagagtatgc
    10381 ttcgtcatga ccgttcctta agggcgtgcg aggaaattca gcaccagttg attgaaagcg
    10441 tcggcatgtt cccactgcgc ccagtgacca cagtcgcgga agatatgcag ttcagaaccg
    10501 gcaatgccgg acagcagacg cagacccgca tccatcggca caaagcggtc gttgcgcccc
    10561 cagacaatca gggtttgcgc tttgatttcc gccagacgtg ggccaaaatc cgggaactgt
    10621 ttcggattgg cttccaggct cttaacgaag ttttccaggt gatcgcggcg cgacagcata
    10681 ttattcaggc gcgcttcaaa cagggcgtcg gtcaaatcgc tggtatcaaa aacgaagata
    10741 tccatcatca gcttcaggtt ttcgatagtc ggctgacgat aaagctgatt cagtcgctta
    10801 ataccttcgg ttggcatcgg cgtaaacaaa ctcatgccgc ccgtaccgcc gcccatcagc
    10861 accagtttgc cgacgcgctc cggccagtta agggtgaacg ccacagaact gtggccgccc
    10921 atcgagttgc ccagcaggtg gattttggcg atatccagtt gatccaccac gcttttcagg
    10981 attcgcgcat taagatccga tcgtgaacca ctattaacga tcgaatcgct cttaccccaa
    11041 cccggacaat ccagcaggat cacccgatag cccgccttta ccagcggatc gatattgcgg
    11101 ctgaagttcg cccagccagt agcacccggg ccggaaccat gcagcaggac aacagtttcg
    11161 tcgccttgtc cgcagtcatt aaaatggatg cgcagcgttt tacccgcttc ttctacattc
    11221 agaaaacggc tggtggcggc ttcggtttgt ggctgataac tcatcatctt ctcctgcata
    11281 ttcagttctc tgttctggcg cttaacgagc caaatccggc aatccactcc gggattgggc
    11341 ggtaataacg cccttcgcta cgccagttgc caaacgcaga aatagcggca aaagcggcga
    11401 cccaggtttt gatttcatgt gtcgactttc cggcaatggc ggaaagctct tcgttactga
    11461 cggcatccag ttcctgtatg cgtccctgct ccagcaaagt catgaactgg ttatcccaaa
    11521 tcgggttgag cggatgcagc gttctctgat cctcaacaaa cttctcagcg gcgctaatca
    11581 cccgttgctg acgcaattcg cgctcactgg cgggtaaatc tttcccgctc cccaacagac
    11641 ggtcgcgcat atgggcatcg gctttcgcca gttctggcac cggcggctga tgggaaagcc
    11701 caccggaacc caggaacagc acgcgtttat tgagagtact ggtgaaacgt ccaatggctt
    11761 cccccaacat gcgggtacgc tggaaaccgg gcaacggcgt ggcgacaccg ttgatgaaca
    11821 caggcagaac cggcacctta tccagcccac cgagcaggaa ctccagcggc tgggcgaacc
    11881 cgtggtccac ctgcatacag taagaaacgg caagatcgat tccgctcttc atgacggcat
    11941 gcgcacaggc ctccgccagc tcaacaggca cgggcagctc tcctgccgca ctaccgaaat
    12001 caccaattgc cgtcgctcca acgcctaaac agaacggtgg catcacgtca tagaaaaagc
    12061 cgttgtagtg atctggcgca aacagcacca ccaactcagg ggagaaagcc gcaatacgct
    12121 cgcgggcgct ggcaatcacg ccattgacct catcgagcac ctcttgcgcc gggtcgacat
    12181 atcccaccag cggcgagtgg gaaagacagt gaagataagc gtgcatatca ggctaccttt
    12241 tcgacagaaa cgtcggcatc agggcgggtc agcgtcatta ccgacgccag tttattcagc
    12301 gtattaccca gagtttgcgg aatggcggtg gcggcaacaa agcgatccgg gcgcatcacc
    12361 accagcgaag cattgtgttg cgcgaaccag ctacgcaggc gaccttgcgt atcgcccacg
    12421 cgtagtacgc cgtcgtggtt atcctgtgcg gtatgaattt gcacttccgg caccacctga
    12481 atgaagcgtg tgcccaacgc gcgccactgc tggatttgct cgtcgctcat cccccacagt
    12541 ggattgcatc cccagccaat taccgcgaag ttcgcgccga tcgcgttatc gagcagcgtc
    12601 acgtcgccgt tttccagcgt gactttcggc tgaataaaca tcttgccgac cggagagtgc
    12661 ttcgcttcgc cctcacgcac cagcgcaccg ccgtaatatt gcggcatcgg cttgaagcgc
    12721 atttcgagga agtagcgttt tactggcggc agataattca acagccagga aacgccgtca
    12781 cgtaacgtac cctgccagcg tttcggcgga gccagcacgt tgcccgccgt cacggacagg
    12841 tcaatcatcg ctttggcgtg atcgcgacgt tcttgttgat aggtatcgag cagcgcatcg
    12901 cgggctttcc cctggataac caacgccagt ttccatgcga ggttaaaggc gtcgcgcata
    12961 ccactgttat agccctgccc ctgccacacc ggcatgatgt gcgcggcatc gcccgccagc
    13021 agtacgcgat caatacgaaa acgttgcgcc agtcgcgcgt tgtgggtgta gacacgctgg
    13081 cgaatcaatt caacattgtc cggattaggc agcactttgc ttaacagctt gcgcatattt
    13141 tgcggctcac gcagctgttc ttcggtttct cccggcatca ccataaattc aaagcgacgt
    13201 accgcatgag gcagcgcggc agaaacatac gggcgcaccg gatcacaaca caaatagata
    13261 tgcggcgtac ttaacggatc gttggcgata tctaccacaa tccactgatt tggcgcagtt
    13321 ttaccttcaa acggcacatt cagggtgcga cggacaaaac ttgccccacc atcacaggcc
    13381 accagccatt gggctttgac cgtttcccgc tgcccttctg ccgttttcag gtgcaaggtc
    13441 acttcgtcat cttgctggct gaaggcctcc agctcgcggg aaaacaggca gcgcacattc
    13501 ggaaaacgcg acaatccttc cagcatcacc gcatcgacct gaggctgaat aaaggcgtta
    13561 cggcgcggcc agccaaattc atcggtcatt ggctgaatat cagcaaaaca gcgacctttt
    13621 ggggtgagaa aacgcatcgc gtgccacggc gtagtgtgcg gcagaacatt atcgaccagg
    13681 ccgaccgact gcatggtgcg cagcgcctcg tcatcaatac caatcgcacg cgggtagtcg
    13741 atcaacttat cgagtttctc caccaccagc acgtcaatgc ccatctggcc gagatagttc
    13801 gccatcatca gcccgaccgg accggcacca gcgatcgcca cctgaacgct atggttaaca
    13861 gcaggctgga tgtcaggatg ttgtattgcc atttcagtac ctcacgactc ggacaaaatg
    13921 ccgttgcgcg cacagtacgg cgcaacttat tttgttaaaa acatgtaaat gattttttat
    13981 tgtgcgctca gtataggaag ggtgttttcg gctacaatca aaacatgccc gaatgtgcac
    14041 caagtgcaca acgttgtttt aactatagaa atgtcaatta atatgcagaa caatgagcag
    14101 acggcataca aaaccgtgcg cggcttaacc cgcggtctaa tgttattaaa tatgttaaat
    14161 aaacttgatg gcggtgccag cgtcgggctg ctggcggaac tcagcggcct gcatcgcacc
    14221 actgtgcggc gactgctgga gacgctgcag gaagagggat atgtccggcg tagtccctcc
    14281 gatgatagtt tccgtttgac catcaaagtg cggcaattaa gcgaaggatt tcgtgacgaa
    14341 cagtggattt ctgcactggc ggccccactg ctgggcgatc tgttgcgcga agtggtatgg
    14401 ccgacagatg tgtccaccct ggatgttgat gcaatggtgg tacgcgaaac tacccatcgt
    14461 ttcagccgct tatcttttca ccgggcaatg gtcgggcgac ggttaccgct tctgaaaacc
    14521 gcctcgggcc tgacctggct ggccttttgc ccggaacaag aacgcaagga attaatcgag
    14581 atgttagcct cccgccccgg tgatgactat caactggcac gggaaccgtt aaagctggaa
    14641 gccattctgg cgcgcgcgcg caaagagggg tacggacaga actaccgcgg ctgggatcaa
    14701 gaagaaaaga tcgcctctat tgccgtaccg ctgcgcagtg aacaacgggt gattggctgt
    14761 ctgaatctgg tgtatatggc gagcgcaatg accattgaac aggcagcgga aaagcatctt
    14821 ccggcgctac aacgggtggc aaaacagatc gaagaagggg ttgaatcgca ggctattctg
    14881 gtggccggac ggcgaagcgg cgtgcattta cgttgacacc atcgaatggc gcaaaacctt
    14941 tcgtggtatg gcatgatagc gcccggaaga gagtcaattc agggtggtga atgtgaaacc
    15001 agtaacgcta tacgatgtcg cagagtatgc cggtgtctct tatcagaccg tttcccgcgt
    15061 ggtgaaccag gcctgccacg tttctgcgaa aacgcgggaa aaagtggaag cggcgatggc
    15121 ggagctgaat tacattccca accgcgtggc acaacaactg gcgggcaagc agtcgttgct
    15181 gattggcgtt gccacctcca gtctggccct gcacgcgccg tcgcaaattg tcgcggcgat
    15241 taaatctcgc gccgatcaac tgggtgccag cgtggtggtg tcgatggtag aacgaagcgg
    15301 cgtcgaagcc tgtaaagcgg cggtgcacaa tcttctcgcg caacgcgtca gtgggctgat
    15361 cattaactat ccgctggatg accaggatgc cattgctgtg gaagctgcct gcgctaatgt
    15421 tccggcttta tttcttgatg tctctgacca gacacccatc aacagtatta ttttctccca
    15481 tgaagacggt acgcgactgg gcgtggagca tctggtcgca ttgggtcacc agcaaatcgc
    15541 gctgttagca ggtccattaa gttctgtctc ggcgcgtctg cgtctggcgg gctggcataa
    15601 atatctcaca cgcaatcaaa ttcagccgat agcggaacgg gaaggcgact ggagtgccat
    15661 gtccggtttt caacaaacca tgcaaatgct gaatgagggc atcgttccca ctgcgatgct
    15721 ggttgccaac gatcagatgg cgctgggcgc aatgcgcgcc attaccgagt ccgggctgcg
    15781 cgttggtgcg gatatctcgg tagtgggata cgacgatacc gaagacagct cgtgttatat
    15841 cccgccgtta accaccatca aacaggattt tcgcctgctg gggcaaacca gcgtggaccg
    15901 cttgctgcaa ctctctcagg gccaggcggt gaagggcaat cagctgttgc ccgtctcact
    15961 ggtgaaaaga aaaaccaccc ttccgcccaa tacgcaaacc gcttctcccc aggcgttggc
    16021 agattcttta atgcagctgg cgcgacaaat ttcccgactc gaaagcgggc agtgagcgca
    16081 acgcaattaa tgtgagtcag ctcactcatt aggcacccca ggctttacac tttatgcgtc
    16141 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga tacagctatg
    16201 actatgatta cggattcact ggccgtcgta ttacaacgtc gtgactggga aaaccctggc
    16261 gttacccaac ttaatcgcct tgcggcacat ccccctttcg ccagctggcg taatagcaaa
    16321 gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atggcgcttt
    16381 gcctggtttc cggcaccaga agcggtgccg gaaagctggc tggagtgcga tcttcctgac
    16441 gccgatactg tcgtcgtccc ctcaaactgg cagatgcacg gttacgacgc gcccatctac
    16501 accaacgtga cctatcccat tacggtcaat ccgccgtttg ttcccgcgga gaatccgaca
    16561 ggttgttact cgctcacatt taatgttgat gaaagctggc tacaggaagg ccagacgcga
    16621 attatttttg atggcgttaa ttcggcgttt catctgtggt gcaacgggcg ctgggtcggt
    16681 tacggccagg acagccgttt gccgtctgaa tttgacctga gcgcattttt acgcgccgga
    16741 gaaaaccgcc tcgcggtgat ggtactgcgc tggagtgacg gcagttatct ggaagatcag
    16801 gatatgtggc gaatgagcgg cattttccgt gacatctcgt tgctgcacaa accgaccaca
    16861 caaatcagcg atttccatgt taccactcgc tttaatgatg atttcagccg cgcggtactg
    16921 gaggcagaag ttcaggtgta cggcgagctg cgcgatgagc tgcgggtgac ggtttctttg
    16981 tggcagggtg aaacgcaggt cgccagcggc accacgcctt tcggcggtga aattatcgat
    17041 gagcgtggtg gttatgccga tcgcgtcacg ctacgtctga acgtcgaaaa cccggcgctg
    17101 tggagcgccg aaatcccgaa tatctatcgt gcggtggttg aactgcacac tgccgacggc
    17161 acgctgattg aagcagaagc ctgcgatgtc ggtttccgcg aggtgcggat tgaaaatggt
    17221 ctgctgctgc tgaacggcaa gccgttgctg attcgcggcg ttaaccgtca cgagcatcat
    17281 cctctgcatg gtcaggtcat ggatgagcag acgatggtgc aggatatcct gctgatgaag
    17341 cagaacaact ttaacgccgt gcgctgttcg cattatccga accatccgct gtggtacacc
    17401 ctgtgcgacc gctacggcct gtatgtggtg gatgaagcca atattgaaac ccacggcatg
    17461 gtgccaatga atcgtctgac cgatgatccg cgctggttac cggccatgag cgaacgcgta
    17521 acgcgaatgg tgctgcgcga tcgtaatcac ccgagtgtga tcatctggtc gctggggaat
    17581 gaatcgggcc acggcgctaa tcacgacgcg ctgtatcgct ggatcaaatc tgtcgatcct
    17641 tcccgcccgg tgcagtatga aggcggcgga gccgacacct ccgcaaccga tattatttgc
    17701 ccgatgtacg cgcgcgtgga tgaagaccag ccctttccgg ctgtgccgaa atggtccatc
    17761 aaaaaatggc tttcgctgcc tggagaactg cgcccgctga tcctttgcga atatgcccac
    17821 gcgatgggta acagtcttgg cggcttcgct aaatactggc aggcatttcg tcagtacccc
    17881 cgtttacagg gcggcttcgt ctgggactgg gtggatcagt cgctgattaa atatgatgaa
    17941 aacggcaacc cgtggtcagc ttacggcggt gattttggcg atacgccaaa cgatcgccag
    18001 ttctgcatga acggtctggt ctttgccgac cgcacgccgc atccggcgct gacggaagca
    18061 aaacaccagc agcagttttt ccagttccgt ttatccgggc gaaccatcga agtgaccagc
    18121 gaatacctgt tccgtcatag cgataacgag ctcctgcact ggtcggtggc gctggatggc
    18181 aagccgctgg caagcggtga aatgcctctg gatgttgctc cacaagataa acagttgatt
    18241 gaactgcctg aactaccgca gccggagagc gccggacaac tctggcttac ggtacacgta
    18301 gtgcaaccga acgcgaccgc atggtcagaa gccggacaca tcagcgcctg gcagcaatgg
    18361 cgtctgacgg aaaacctcag cgtgacactc ccctccgcgc cccacgccat cccgcaactg
    18421 accaccagcg aaacggattt ttgcatcgag ctgggtaata aacgttggca atttaaccgc
    18481 caatcaggct ttctttcaca gatgtggatt ggcgatgaaa aacaactgct gacgccgctg
    18541 cgcgatcagt tcacccgcgc accgctggat aacgacattg gcgtaagtga agcgacccgc
    18601 attgacccta acgcctgggt agaacgctgg aaggcggcgg gccattacca gaccgaagtg
    18661 gcgttgttgc agtgcacggc agatacactt gccgacgcgg tgctgattac gaccgctcac
    18721 gcgtggcagc atcaggggaa aaccttattt atcagccgga aaacctaccg gattgatggt
    18781 agtggtcaaa tggcgattac cgttgatgtt gaagtagcga gcgatacacc gcatccggca
    18841 cggattggcc tgacctgcca gctggcgcag gtcgcagagc gggtaaactg gctcggatta
    18901 gggccgcaag aaaactatcc cgaccgcctt actgccgcct gttttgaccg ctgggatctg
    18961 ccattgtcag acatgtatac cccgtacgtc ttcccgagcg aaaacggtct gcgctgcggg
    19021 acgcgcgaat tgaattatgg cccacaccag tggcgcggcg acttccagtt caatatcagt
    19081 cgctacagcc aacaacaact gatggaaacc agccatcgcc atctgctgca cgcggaagaa
    19141 ggcacatggc tgaatatcga cggtttccat atggggattg gtggcgacga ctcctggagc
    19201 ccgtcagtgt cggcggaatt ccagctgagc gccggtcgct accattacca gttggtctgg
    19261 tgtcaaaaat aataataacc gggcaggcca tgtctgcccg tatttcgcgt aaggaaatcc
    19321 attatgtact atttaaaaaa cacaaacttt tggatgttcg gtttattctt tttcttttac
    19381 ttttttatca tgggggccta cttcccgttt ttcccgattt ggctacatga catcaaccat
    19441 atcagcaaaa gtgatacagg tattattttt gccgctattt ctctgttctc gctattattc
    19501 caaccgctgt ttggtctgct ttctgacaaa ctcgggctgc gcaaatacct gctgtggatt
    19561 attaccggca tgttagtgat gtttgcgccg ttctttattt ttatcttcgg gccactatta
    19621 caatacaaca ttttagtagg atcgattgtt ggtggtattt atcttggctt ttgttttaac
    19681 gccggtgcgc cagcagtaga ggcatttatc gagaaagtca gccgtcgcag taatttcgaa
    19741 tttggtcgcg cgcggatgtt tggctgtgtt ggctgggcgc tgtgtgcctc gattgtcggc
    19801 atcatgttca ccatcaataa tcagtttgtt ttctggctgg gttctggctg tgcactcatc
    19861 ctcgccgttt tgctcttttt cgccaaaacg gatgcgccct cttccgccac ggttgccaat
    19921 gcggtaggtg ccaaccattc ggcatttagc cttaagctgg cgctggaact gttcagacag
    19981 ccaaaactgt ggtttttgtc actgtatgtt atcggcgttt cctgcaccta cgatgttttt
    20041 gaccaacagt ttgctaattt ctttacttcg ttctttgcca caggtgaaca gggtacgcgg
    20101 gtatttggct acgtaacgac aatgggcgaa ttacttaacg cctcgattat gttcttcgcg
    20161 ccgctgatca ttaatcgcat cggtgggaaa aacgccctgc tgctggctgg cactattatg
    20221 tctgtacgta ttattggctc atcgttcgcc acctcagcgc tggaagtggt tattctgaaa
    20281 acgctgcata tgtttgaagt accgttcctg ctggtgggct gctttaaata tattaccagc
    20341 cagtttgaag tgcgtttttc agcgacgatt tatctggtct gtttctgctt ctttaagcaa
    20401 ctggcgatga tttttatgtc tgtactggcg ggcaatatgt atgaaagcat cggtttccag
    20461 ggcgcttatc tggtgctggg tctggtggcg ttgggcttca ccttaatttc cgtgttcacg
    20521 cttagcggcc ccggtccgct ttctctactg cgtcgtcagg tgaatgaagt cgcttaagca
    20581 attaatgtcg gatgcggcgc gagcgcctta tccgaccaac atatcaggac ggaatgatcg
    20641 caatgaacat gccaatgacc gaaagaataa aagcaggcaa gctatttacc gatatgtgcg
    20701 aaggcttacc ggaaaaaaga cttcgtggga aaacgttaat gtatgagttt aatcactcgc
    20761 atccatcaga agttgaaaaa agagaaagcc tgattaaaga aatgtttgcc acggtagggg
    20821 aaaacgcctg ggtagaacca cccgtccatt tctcttacgg ttccaacatc catataggcc
    20881 gcaattttta tgcaaatttc aatttaacca ttgtagacga ctacacggta acgatcggtg
    20941 ataacgtatt gattgcaccc aatgttactc tttccgttac gggacaccct gtacaccatg
    21001 aattgagaaa aaacggtgag atgtactctt ttccgataac gattggcaat aacgtctgga
    21061 tcggaagtca tgtggtcatt aatccaggcg tcaccatcgg ggataattct gttattggcg
    21121 cgggtagtgt cgtcataaaa gacattccac caaacgtcgt ggctgctggc gttccttgcc
    21181 gggttattcg cgaaataaac gaccgggata agcactatta ttacaaagat tataaagttg
    21241 aatcgtcagt ttaaattata aaaattgcct gatgcgctcc gcttatcagg cctacaagtt
    21301 cagtgatcta cgtaggccgc atccaacgta aacaaagcgc aggaacaagc gtcgcatcat
    21361 gcctctttga cccacagctg cggaaaacgt gctggtgcaa aacgcagggt tatgatcatc
    21421 aacccaacga cgcacagcgc atgaaacgcc cagtccatca agtaattgcc actgatacta
    21481 cgcagcacac cagaaaacca cggggcaagc ccggcgatga taaaaccgat cccctgcata
    21541 aacgccacca gtttgccagc aatagcgggt tgcgcagagt gatcgagtgc cagcagcaaa
    21601 cagagcggaa atgcgccgcc cagacctaaa ccacacacca tcgcccagaa gaccggcaat
    21661 tgcagcggca gccagataaa gccgcagaaa cccaccagtt gtaacgccag cgccagcatc
    21721 aaaagtttgc gccgatcctg atggcgagcc atagcaggca tcagcaatgc ccccgcagct
    21781 tgcccaagcg tcatcaacgc cagcaaggaa ccgctgtact gcgcgctggc accaatctca
    21841 atatagaaag cgggtaacca ggcaatcagg ctggcgtaac caccgttaat cagaccgaag
    21901 taaacaccca gcgtccacgc gcggggagta aataccacgc gaaccggagt ggttgttgtc
    21961 ttgtgagaag aggcgacctc gcgggcgttt tgccaccacc aggcaaagag cgcaacaacg
    22021 gcaggcagcg cccaccaggc gagtgtttga taccaggttt cgctatgttg aactaaccag
    22081 ggcgtaatgg cggcaccaag tccaccgccg cccattagag ccgcggacca cagccccatc
    22141 accagtggcg tacggtgctg aaaccgccgc ttaattaccg aaggcatcac tgcctgaatg
    22201 atgccgatcc ccactccacc aagcagtgcg ctgctaagca gcagcgcact ttgggggtaa
    22261 agctcacgca tcaatgcacc gacggcaatc agtaacaaac tgatggcgac gctgcgacgt
    22321 tcgctgacat gctgatgaag ccagcttccg gccagcgcca gcccgcccat ggtaaccacc
    22381 ggcagagcgg tcaacagggc agccacgcta aagctcattc cgcttgcctg gcgcaattgc
    22441 ggtagcagtg gcccgacgga ggtgagcagt ggtcgcatat taagaccaat cagcaccaga
    22501 accagcagca tcgtgaacgt cttttgatgg ttacgcatgg ctgtcagaac ggtttggtcg
    22561 gcagatattt accatctaag gtgatgaccg cacgttcgcc accctccggg tccgccactt
    22621 tcttaacgtc gagtttgaag ttaatcgcgc tgataatgcc atcgccaaac ttctcatgaa
    22681 ccaacgcttt cagggttgtg ccgtacacct gcaacatttc atagaaacga tacatcgttg
    22741 ggtcggttgg aatacggtca tcaatgcagc cacgcagtgg aatcatctgc aacagtagaa
    22801 tggcgtcttc gtcgagatcc agcttcgctc cgaccaggcg ggcggcgtcg gcaggaagcg
    22861 cctgctgccc cagcaaagcc gcggttacaa aggcttctgc cagaccggtg ccgtcggcaa
    22921 tctcggcaaa tgagagatct tttttagctt tgctgagcaa aatggcatcg gcaagatcaa
    22981 gacgaatatt gcggttaatt tgcgactgaa tcatggtgga tctcctgatg gtttaaaaat
    23041 aaggttacgc tgcggtcggt tggcgtagcg gtatggcaca aacgcgagga ttagcggcca
    23101 gtggcacaaa ctggcgggtt gcgccgtcaa aagctgcgat gctgccgctt tcaatgtcgt
    23161 agacccagcc gtgcagggca atccgcccct cttcgagcgc caggcgcacc gatggatgag
    23221 tttgcaaatt agccaactga gcaatgacgt tttcacgtac catcgccgct gcttttgacg
    23281 gtaaatcggg atgcgggcgc gcctcattaa cgacgcgggc tgaatcggca taacgcagcc
    23341 agtgggagac ggcaggcata tggtccatgc actgacagct ggcaatggag gtcatcgcgc
    23401 cacagttgga atgaccgcaa atcacaatgt cagatacccg aagtgcagcg acggcatact
    23461 ccaccgaagc agaaacgcca ccgggttccg gcccgtagga agggacgata ttgcccgcgt
    23521 tgcgaataac gaacagatcg ccaggctcac gttgcgtcac cagctcaggg accagacggc
    23581 tgtcggagca ggagataaaa agggtgcgcg ggctttgctg tgtcgccagc tgtttaaaca
    23641 aggcttcccg cttcggaaat gcctcgcgct ggaatttaag gaatccatca ataatctctt
    23701 tcaccgttaa cctctgtctg tctctggaat gagaggcaga ctactcgccg attgtcataa
    23761 ggtaaaagac tcatttatga tgagttctat tggatttact tataggttgg cgaatgctct
    23821 ctcgacatat caattacttt cttgccgtgg ctgaacatgg cagcttcacc cgtgcagcca
    23881 gtgcgttgca cgtctcccag cctgcgcttt cccagcagat tcgccagtta gaggaaagtt
    23941 tgggcgtgcc gctgtttgac cgtagcgggc gaacgattcg tctcactgat gcaggagaag
    24001 tctggcgaca gtacgccagc cgggcgttac aggaactggg ggcgggtaaa cgggcgattc
    24061 atgatgttgc cgatctgacg cgaggatcgc tgcgtatcgc cgtcactccc acctttacaa
    24121 gctactttat cggcccttta atggcggatt tctatgcgcg ctaccccggc atcacgctcc
    24181 agctacagga aatgtcgcag gagaaaatcg aagatctgct ttgccgcgac gagttggacg
    24241 ttgggattgc cttcgcgcct gtgcattcgc cggagctgga ggcaattcct ttactgacag
    24301 aaagtttagc gttagtcgtg gcgcaacatc atccgctggc tgcctgtgaa caggtggcgt
    24361 tgagtcgctt gcatgatgaa aaactggtcc tgctcagcgc ggaatttgcc accagagagc
    24421 aaattgacca ctactgcgag aaagcggggc tacatccaca ggtggtcatt gaggcgaact
    24481 caattagcgc ggttctggag ctgattcgcc gcacttccct ttccacattg ttgccagcag
    24541 cgattgccac acaacatgac gggcttaaag ctatatccct tgccccgcca ctactggaga
    24601 gaacggcggt tttgttgcgg cggaaaaata gctggcagac agccgccgcg aaggcatttt
    24661 tgcacatggc gttggataaa tgcgcggttg ttggcggaaa tgaatcgcgg taaccactga
    24721 cacaattgcc tgatgcgcta cgcttatcag gcctacgagt tcagtgctct acgtaggccg
    24781 gataaggcgt tcacgccgca tccggcataa actacgctcg ctttgtcaac acacagccca
    24841 gccgatcacc gtttgtaatc gatggcttct ggctgctcca gatatacggt ggtttgtgct
    24901 ggttgggtgc tggcaatcac cttaccgcca cgtaccgaat aacgtacagg aacctgacgg
    24961 cgcagcgcgt caaacccatt ttcagccggc aggataatca ggttggcgct gtttccggcg
    25021 gcaatgccgt aatcctgcaa attcaacgtc ctggcactgt ggtgggtgat taaattcagg
    25081 ccatcgttaa tctgcccgta gcccatcagc tggcaaacat gcagccccat atgcagcact
    25141 tgcagcatat tcgccgttcc cagcggatac cacggatcga agacatcatc gtgaccaaag
    25201 cagacgttaa tgccggactc cagcatctct ttaacgcgcg tgatgccgcg acgttttgga
    25261 taggtatcga aacgtccttg cagatggata ttgaccagcg ggttggcgac aaagttaata
    25321 ccggacattt tcagcaagcg gaacaggcgt gaggtatacg ccccgttata ggagtgcatt
    25381 gccgtggtgt ggctggcggt gactcgcgcg cccattcctt cacggtgcgc cagggcagca
    25441 acggtttcga caaagcgcga ctgctcgtca tcgatctcat cacagtgaac gtcgatgaga
    25501 cggtcgtatt tttgcgccag ggcgaaggtt ttatgcagcg actccacgcc gtattcacgg
    25561 gtaaattcaa aatgcggaat cgcccccact acgtcagccc ctaagcgtaa cgcctcttcc
    25621 agcaacgctt caccgttggg atacgacaaa atcccttcct gcgggaaggc gacgatttgc
    25681 agatcaatcc acggcgcgac ttcctgcttc acttccagca ttgctttcag cgcagtcagc
    25741 gttgcatccg aaacatcgac atgggtacgc acatgctgaa tgccgttggc aatctgccat
    25801 ttcagcgttt gccaggcgcg ttgtttcaca tcgtcatggg ttaataacgc tttgcgctcg
    25861 gcccagcgtt caatgccttc aaacagcgtg ccggactgat tccagttcgg ttgtccggcg
    25921 gtttgcgtgg tgtccaggtg aatatgcggc tccacaaacg gcggtataac taaaccttgt
    25981 tcggcatcca ggctgttttc agttatgggc atcaccccgg attgcgcatc aatggcgctg
    26041 atttttccgt ccagcagatg aatctgccac agcccctctt tgccgggtaa ccgggcgtta
    26101 ataattgtct gtaaagcgtt attcgacact gttagcctcc acatgcgtca ttgcagccgt
    26161 cgttttgcga ttcaaaatcg ggttaaggat cagatagctc agcgcgccac ctaataccgc
    26221 gttgaccgga acaatacccg gtaaccagtg gcctgcggca atccccaagg cgaccgccag
    26281 aatcgccacc caattgacac tcatcatacg cgtggtcgca aagtgctcat agcggcgacg
    26341 gttcatcaga tagtcagcga tgatcacgcc ccctaccgga ggaatagctg ccgaaaggaa
    26401 ggtcagccag ccgacaaaat tgttatacag ccataatgcg cagaccgtac cgataatacc
    26461 gttgattacc gaaagggttt tactcgacat accagtaatg ttggcgaaac ctaaacccga
    26521 cgcatagagc gcgttatcgt tggtggtcca gatattcagc cccagcacca caatcgcagg
    26581 cagcagcagg ccctgagcaa tcatcacatc agagatatcc gccatcccca gtgccgcagc
    26641 gcccgctgca ccgaaaataa acatcaacga gttgccgagg aaaaaggcca ccatcgccac
    26701 cagcaccgcc agtttggcat tacgaccaaa ccggacaaag tcagcggtga gcgtgcccgc
    26761 actgataaat gaccccacaa ccagcgccag cgcgacattg aaatctaacg gttgtgcggg
    26821 aacgaccgct tttaatgcgt ccaggccgcc catgccatta acggccagcc acacggaata
    26881 accgccaagg caggcgatag ccggaaccgc aatcaccgaa agaaccgtca gcgccgaaat
    26941 gccaaagaag acggtgacgg tcatcagtaa accggaaacg gcaatcagca aattaatatc
    27001 cagcccggtt gccttgccca ccggaatggc aaacatcgcc acgccaacgc caaaccagcc
    27061 aacctgggtg ccgcccagca gcagtgaagg cagccatgag cctttaacac caaaagagaa
    27121 gcgagcaaga agatgagtgg tcaggccggt ttttgcgcca atgtaaccaa gaaatgaagt
    27181 gtaaataccg aggagaagat taccgatgag gactgcgagg aagaaatcat gatagctaag
    27241 accggttccg agagtgccgc cggtccacat actggcggaa aagaaggtta atcccagcat
    27301 gacgaacgtc aatgccaata cccctttccg cgccgactgc gggactggcc cctggctaaa
    27361 gttgttatct tgcgacacga aattccccct ttttgctgtt tgaaacccaa aaaatccgcc
    27421 gcattctatt catctgaaga taaaaagcaa tcgttttcgt aggggaatat atttcttata
    27481 tgaatgagta caaaaaggcg ggggaaactt atctgtgtaa aaacagtggg taggcatgat
    27541 aagacgcgtc agcgtcgcat caggcatctg cgca
//
LOCUS       DAZFLD010000045        27519 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.045, whole genome shotgun sequence.
ACCESSION   DAZFLD010000045 DAZFLD010000000
VERSION     DAZFLD010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27519
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.045"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            116..358
                     /gene="yjbE"
                     /locus_tag="ACHKBL_001602"
     CDS             116..358
                     /gene="yjbE"
                     /locus_tag="ACHKBL_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide production protein YjbE"
                     /protein_id="HFO5818485.1"
                     /translation="MKKVLYGIFAISALAATSAWAAPVQVGEAAGSAATSVSAGSSSA
                     TSVSTVSSAVGVALAASGGGDGSNTGTTTTTTTSTQ"
     gene            545..1183
                     /gene="yjbF"
                     /locus_tag="ACHKBL_001603"
     CDS             545..1183
                     /gene="yjbF"
                     /locus_tag="ACHKBL_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HFO5818486.1"
                     /translation="MKRPALILICLLLQACSATTKELGNSLWDSLFGTPGVQLTDDDI
                     QNMPYASQYMQLNGGPQLFVVLAFAEDGQQKWVTQDQATLVTQHGRLVKTLLGGDNLI
                     EVNNLAADPLIKPAQIVDGATWTRTMGWIEYQQVRYATARSVFKWDGTDTVKVGSDET
                     PVRVLDEEVSTDQVRWHNRYWIDSEGQIRQSEQYLGADYFPVKTTLIKAAKQ"
     gene            1180..1917
                     /gene="yjbG"
                     /locus_tag="ACHKBL_001604"
     CDS             1180..1917
                     /gene="yjbG"
                     /locus_tag="ACHKBL_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC D2 domain-containing
                     protein"
                     /protein_id="HFO5818487.1"
                     /translation="MIKQTIVALILSVGASSVFAAGTVKVFSNGSSEAKTLTGAEHLI
                     DLVGQPRLANSWWPGAVISEELATAAALRQQQALLARLAELAADSSADDAAAINALRQ
                     QIQALKVTGRQKINLDPDIVRVAERGNPPLQGNYTLWVGPPPSTVTLFGLISRPGKQP
                     FTPGRDVASYLSGQNLLSGADRSYAWVVYPDGRTQKAPVAYWNKRHVEPMPGSIIYVG
                     LADSVWSETPDALNADILQTLTQRIPQ"
     gene            1917..4013
                     /gene="yjbH"
                     /locus_tag="ACHKBL_001605"
     CDS             1917..4013
                     /gene="yjbH"
                     /locus_tag="ACHKBL_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HFO5818488.1"
                     /translation="MKKRHLLSLLALGISTACYGETYPAPIGPSQSDFGGVGLLQTPT
                     ARMAREGELSLNYRDNDQYRYYSASVQLFPWLETTLRYTDVRTRQYSSVEAFSGDQTY
                     KDKAFDLKLRLWEESYWLPQVAVGARDIGGTGLFDAEYLVASKAWGPFDFTLGLGWGY
                     LGTSGNVKNPLCSASDKYCYRDNSYKQAGSIDGSQMFHGPASLFGGVEYQTPWQPLRL
                     KLEYEGNNYQQDFAGKLEQKSKFNVGAIYRVTDWADVNLSYERGNTFMFGVTLRTNFN
                     DLRPSYNDNARPQYQPQPQDAILQHSVVANQLTLLKYNAGLADPQIQTKGDTLYVTGE
                     QVKYRDSREGIIRANRIVMNDLPDGIKTIRITENRLNMPQVTTETDVASLKNHLAGEP
                     LGHETKLAQKRVEPVVPQSTEQGWYIDKSRFDFHIDPVLNQSVGGPENFYMYQLGVMG
                     TADLWLTDHLLTTGSLFANLANNYDKFNYTNPPQDSHLPRVRTHVREYVQNDVYVNNL
                     QANYFQHLGNGFYGQVYGGYLETMFGGAGAEVLYRPLDSNWAFGLDANYVKQRDWRSA
                     KDMMKFTDYSVKTGHLTAYWTPSFAQDVLVKASVGQYLAGDKGGTLEIAKRFDSGVVV
                     GGYATITNVSKEEYGEGDFTKGVYVSVPLDLFSSGPTRSRAAIGWTPLTRDGGQQLGR
                     KFQLYDMTSDRSVNFR"
     gene            complement(4060..4338)
                     /locus_tag="ACHKBL_001606"
     CDS             complement(4060..4338)
                     /locus_tag="ACHKBL_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5818489.1"
                     /translation="MKRNLIKVVKMKPYFAALMLSVSVLPAYAGPLGTADKADLPQSN
                     VSSPMMAQSLRQPDLQPISTDRKTECFRLYTPDRKPGVNCVPDGSTGH"
     gene            4552..4962
                     /gene="psiE"
                     /locus_tag="ACHKBL_001607"
     CDS             4552..4962
                     /gene="psiE"
                     /locus_tag="ACHKBL_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
                     /protein_id="HFO5818490.1"
                     /translation="MTSLSRPRVEFISTILQTVLNLGLLCLGLILVVFLGKETVHLAD
                     VLFAPEQTSKYELVEGLVVYFLYFEFIALIVKYFQSGFHFPLRYFVYIGITAIVRLII
                     VDHKSPLDVLIYSAAILLLVITLWLCNSKRLKRE"
     gene            complement(5056..5946)
                     /gene="malG"
                     /locus_tag="ACHKBL_001608"
     CDS             complement(5056..5946)
                     /gene="malG"
                     /locus_tag="ACHKBL_001608"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HFO5818491.1"
                     /translation="MAMVQPKSQKTRLFITHLLLLLFIAAIMFPLLMVVAISLRQGNF
                     ATGSLIPEQISWDHWKLALGFSVEQADGRVTPPPFPVLLWLWNSVKVAGISAIGIVAL
                     STTCAYAFARMRFPGKATLLKGMLIFQMFPAVLSLVALYALFDRLGEYIPFIGLNTHG
                     GVIFAYLGGIALHVWTIKGYFETIDSSLEEAAALDGATPWQAFRLVLLPLSVPILAVV
                     FILSFIAAITEVPVASLLLRDVNSYTLAVGMQQYLNPQNYLWGDFAAAAVMSALPITI
                     VFLLAQRWLVNGLTAGGVKG"
     gene            complement(5961..7505)
                     /gene="malF"
                     /locus_tag="ACHKBL_001609"
     CDS             complement(5961..7505)
                     /gene="malF"
                     /locus_tag="ACHKBL_001609"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
                     /protein_id="HFO5818492.1"
                     /translation="MDVIKKKHWWQSDALKWSVLGLLGLLVGYLVVLMYAQGEYLFAI
                     TTLILSSAGLYIFANRKAYAWRYVYPGMAGMGLFVLFPLVCTIAIAFTNYSSTNQLTF
                     ERAQEVLLDRSWQAGKTYNFGLYPAGDEWQLALSDGETGKNYLSDAFKFGGEQKLQLK
                     ETTAQPEGERANLRVITQNRQALSDITAILPDGNKVMMSSLRQFSGTQPLYTLDGDGT
                     LTNNQSGVKYRPNNQIGFYQSITADGNWGDEKLSPGYTVTTGWKNFTRVFTDEGIQKP
                     FLAIFVWTVVFSLITVFLTVAVGMVLACLVQWEALRGKAVYRVLLILPYAVPSFISIL
                     IFKGLFNQSFGEINMMLSALFGVKPAWFSDPTTARTMLIIVNTWLGYPYMMILCMGLL
                     KAIPDDLYEASAMDGAGPFQNFFKITLPLLIKPLTPLMIASFAFNFNNFVLIQLLTNG
                     GPDRLGTTTPAGYTDLLVNYTYRIAFEGGGGQDFGLAAAIATLIFLLVGALAIVNLKA
                     TRMKFD"
     gene            complement(7659..8849)
                     /gene="malE"
                     /locus_tag="ACHKBL_001610"
     CDS             complement(7659..8849)
                     /gene="malE"
                     /locus_tag="ACHKBL_001610"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HFO5818493.1"
                     /translation="MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYN
                     GLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLL
                     AEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPA
                     LDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGL
                     TFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTF
                     KGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYQ
                     DELAKDPRIAATMDNAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQT
                     RITK"
     gene            9214..10329
                     /gene="malK"
                     /locus_tag="ACHKBL_001611"
     CDS             9214..10329
                     /gene="malK"
                     /locus_tag="ACHKBL_001611"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter ATP-binding
                     protein MalK"
                     /protein_id="HFO5818494.1"
                     /translation="MASVQLQNVTKAWGEVVVSKDINLDIHEGEFVVFVGPSGCGKST
                     LLRMIAGLETITSGDLFIGEKRMNDTPPAERGVGMVFQSYALYPHLSVAENMSFGLKL
                     AGAKKEVINQRVNQVAEVLQLAHLLDRKPKALSGGQRQRVAIGRTLVAEPSVFLLDEP
                     LSNLDAALRVQMRIEISRLHKRLGRTMIYVTHDQVEAMTLADKIVVLDAGRVAQVGKP
                     LELYHYPADRFVAGFIGSPKMNFLPVKVTATAIDQVQVELPMPNRQQVWLPVESRDVQ
                     VGANMSLGIRPEHLLPSDIADVILEGEVQVVEQLGNETQIHIQIPSIRQNLVYRQNDV
                     VLVEEGATFAIGLPPERCHLFREDGTACRRLHKEPGV"
     gene            10401..11741
                     /gene="lamB"
                     /locus_tag="ACHKBL_001612"
     CDS             10401..11741
                     /gene="lamB"
                     /locus_tag="ACHKBL_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltoporin LamB"
                     /protein_id="HFO5818495.1"
                     /translation="MMITLRKLPLAVAVAAGVMSAQAMAVDFHGYARSGIGWTGSGGE
                     QQCFQTTGAQSKYRLGNECETYAELKLGQEVWKEGDKSFYFDTNVAYSVAQQNDWEAT
                     DPAFREANVQGKNLIEWLPGSTIWAGKRFYQRHDVHMIDFYYWDISGPGAGLENIDVG
                     FGKLSLAATRSSEAGGSSSFASNNIYDYTNETANDVFDVRLAQMEINPGGTLELGVDY
                     GRANLRDNYRLVDGASKDGWLFTAEHTQSVLKGFNKFVVQYATDSMTSQGKGLSQGSG
                     VAFDNEKFAYNINNNGHMLRILDHGAISMGDNWDMMYVGMYQDINWDNDNGTKWWTVG
                     IRPMYKWTPIMSTVMEIGYDNVESQRTGDKNNQYKITLAQQWQAGDSIWSRPAIRVFA
                     TYAKWDEKWGYDYTGNANTNTNFGKAVPADFNGGSFGRGDSDEWTFGAQMEIWW"
     gene            11979..12899
                     /gene="malM"
                     /locus_tag="ACHKBL_001613"
     CDS             11979..12899
                     /gene="malM"
                     /locus_tag="ACHKBL_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose operon protein MalM"
                     /protein_id="HFO5818496.1"
                     /translation="MKMNKSLIALCLSAGLLASAPGISLADVNYVPQNTSDAPAIPSA
                     ALQQLTWTPVDQSKTQTTQLATGGQQLNVPGIRGPVAAYSVPANIGELTLTLTSEVNK
                     QTSVFAPNVLILDQNMTPSAFFPSSYFTYQEPGVMSADRLEGVMRLTPALGQQKLYVL
                     VFTTEKDLQQTTQLLDPAKAYAKGVGNSIPDIPDPVARHTTDGLLKLKVKTNSSSSVL
                     VGPLFGSSAPAPVTVGNTAAPAVAAPAPAPVKKSEPMLNDTESYFNTAIKNAVAKGDV
                     DKALKLLDEAERLGSTSARSTFISSVKGKG"
     gene            13078..13575
                     /gene="ubiC"
                     /locus_tag="ACHKBL_001614"
     CDS             13078..13575
                     /gene="ubiC"
                     /locus_tag="ACHKBL_001614"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008813 - chorismate lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HFO5818497.1"
                     /translation="MSHPALTQLRALRYFTEIPALEPQLLDWLLLEDSMTKRFEQQGK
                     TVSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSG
                     PELALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLP
                     ASPLY"
     gene            13588..14460
                     /gene="ubiA"
                     /locus_tag="ACHKBL_001615"
     CDS             13588..14460
                     /gene="ubiA"
                     /locus_tag="ACHKBL_001615"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HFO5818498.1"
                     /translation="MEWSLTQNKLLAFHRLMRTDKPIGALLLLWPTLWALWVATPGVP
                     QLWILAVFVAGVWLMRAAGCVVNDYADRKFDGHVKRTANRPLPSGAVTEKEARALFVV
                     LVLISFLLVLTLNTMTILLSIAALALAWVYPFMKRYTHLPQVVLGAAFGWSIPMAFAA
                     VSESVPLSCWLMFLANILWAVAYDTQYAMVDRDDDVKIGIKSTAILFGQYDKLIIGIL
                     QIGVLALMAIIGELNGLGWGYYWSILVAGALFVYQQKLIANREREACFKAFMNNNYVG
                     LVLFLGLAMSYWHF"
     gene            complement(14615..17038)
                     /gene="plsB"
                     /locus_tag="ACHKBL_001616"
     CDS             complement(14615..17038)
                     /gene="plsB"
                     /locus_tag="ACHKBL_001616"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004366 - glycerol-3-phosphate
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HFO5818499.1"
                     /translation="MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYV
                     LPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESI
                     KLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
                     RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFN
                     KLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWN
                     RLYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGIN
                     LNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTG
                     RLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLP
                     QMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNI
                     AADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDST
                     VPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMA
                     AIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPGVIDALANEMQRQGLITLQ
                     DDELHINPAHSRTLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLS
                     VLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLT
                     IESATQSEG"
     gene            17209..17577
                     /gene="dgkA"
                     /locus_tag="ACHKBL_001617"
     CDS             17209..17577
                     /gene="dgkA"
                     /locus_tag="ACHKBL_001617"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFO5818500.1"
                     /translation="MANNTTGFTRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVV
                     IACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAV
                     LIAIIVAVITWCILLWSHFG"
     gene            17687..18295
                     /gene="lexA"
                     /locus_tag="ACHKBL_001618"
     CDS             17687..18295
                     /gene="lexA"
                     /locus_tag="ACHKBL_001618"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HFO5818501.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAE
                     EHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDP
                     SLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKK
                     QGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     gene            18368..19693
                     /gene="dinF"
                     /locus_tag="ACHKBL_001619"
     CDS             18368..19693
                     /gene="dinF"
                     /locus_tag="ACHKBL_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter DinF"
                     /protein_id="HFO5818502.1"
                     /translation="MAFLTSSDKALWHLALPMIFSNITVPLLGLVDTAVIGHLDSPVY
                     LGGVAVGATATSFLFMLLLFLRMSTTGLTAQAYGAKNPQALARALVQPLLLALGAGAL
                     IALLRTPIIDLALHIVGGSEAVLEQARRFLEIRWLSAPASLANLVLLGWLLGVQYARA
                     PVILLVVGNILNIVLDVWLVMGLHMNVQGAALATVIAEYATLLIGLLMVRKILKLRGI
                     SGEMLKTAWRGNFRHLLALNRDIMLRSLLLQLCFGAITVLGARLGSDIIAVNAVLMTL
                     LTFTAYALDGFAYAVEAHSGQAYGARDGSQLLDVWRAACRQSGIVALLFSVVYLLAGE
                     HIIALLTSLTQIQQLADRYLIWQVILPLVGVWCYLLDGMFIGATRAAEMRNSMAVAAA
                     GFALTLLTLPWLGNHGLWLALTVFLALRGLSLAAIWRRHWRNGTWFAAT"
     gene            19809..20018
                     /gene="yjbJ"
                     /locus_tag="ACHKBL_001620"
     CDS             19809..20018
                     /gene="yjbJ"
                     /locus_tag="ACHKBL_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFO5818503.1"
                     /translation="MNKDEAGGNWKQFKGKVKEQWGKLTDDDMTIIEGKRDQLVGKIQ
                     ERYGYQKDQAEKEVDSWEKRHDYRW"
     gene            complement(20060..20575)
                     /gene="zur"
                     /locus_tag="ACHKBL_001621"
     CDS             complement(20060..20575)
                     /gene="zur"
                     /locus_tag="ACHKBL_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc uptake transcriptional repressor Zur"
                     /protein_id="HFO5818504.1"
                     /translation="MEKTTTQELLAQAEKICAQRNVRLTPQRLEVLRLMSLQDGAISA
                     YDLLDLLREAEPQAKPPTVYRALEFLLEQGFVHKVESTNSYVLCHLFDQPTHTSAMFI
                     CDRCGAVKEECAEGVEDIMHTLAAKMGFALRHNVIEAHGLCSACVEVEACRHPEQCHH
                     DHSIQVKKKPR"
     gene            20731..21726
                     /gene="dusA"
                     /locus_tag="ACHKBL_001622"
     CDS             20731..21726
                     /gene="dusA"
                     /locus_tag="ACHKBL_001622"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HFO5818505.1"
                     /translation="MPAESHTVYPAYRFSIAPMLDWTDRHCRYFLRLLSRNTLLYTEM
                     VTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEARGYDEINLNVGCPS
                     DRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDDQDSYEFLCDFINTV
                     SGKGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDFPHLTMSINGGIKSL
                     EEAKAHLQHMDGVMVGREAYQNPGILAAVDREIFGSSDTDVDPVAVVRAMYPYIEREL
                     SQGTYLGHITRHMLGLFQGIPGARQWRRYLSENAHKAGADINVLEHALKLVADKR"
     gene            21860..22102
                     /gene="pspG"
                     /locus_tag="ACHKBL_001623"
     CDS             21860..22102
                     /gene="pspG"
                     /locus_tag="ACHKBL_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response protein PspG"
                     /protein_id="HFO5818506.1"
                     /translation="MLELLFVIGFFVMLMVTGVSLLGIIAALVVATAIMFLGGMLALM
                     IKLLPWLLLAIAVVWVIKAIKAPKVPKYQRYDRWRY"
     gene            complement(22268..23251)
                     /gene="qorA"
                     /locus_tag="ACHKBL_001624"
     CDS             complement(22268..23251)
                     /gene="qorA"
                     /locus_tag="ACHKBL_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HFO5818507.1"
                     /translation="MATRIEFHKHGGPEVLQAVEFTPADPAENEIQVENKAIGINFID
                     TYIRSGLYPPPSLPSGLGTEAAGIVSKVGSGVKHIKAGDRVVYAQSALGAYSSVHNIN
                     ADKAAILPAAISFEQAAASFLKGLTVYYLLRKTYEIKPDEQFLFHAAAGGVGLIACQW
                     AKALGAKLIGTVGTAQKAQSALKAGAWQVINYREENLVERLKEITGGKKVRVVYDSVG
                     RDTWERSLDCLQRRGLMVSFGNSSGAVTGVNLGILNQKGSLYVTRPSLQGYITTREEL
                     TEASNELFSLIASGVIKVDVAEQQKYPLKDAQRAHEILESRATQGSSLLIP"
     gene            23334..24749
                     /gene="dnaB"
                     /locus_tag="ACHKBL_001625"
     CDS             23334..24749
                     /gene="dnaB"
                     /locus_tag="ACHKBL_001625"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFO5818508.1"
                     /translation="MAGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLD
                     NERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGG
                     FAYLAELSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
                     AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKT
                     AGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASL
                     SRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREH
                     GGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQR
                     ADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVR
                     LTFNGQWSRFDNYAGPQYDDE"
     gene            24802..25881
                     /gene="alr"
                     /locus_tag="ACHKBL_001626"
     CDS             24802..25881
                     /gene="alr"
                     /locus_tag="ACHKBL_001626"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HFO5818509.1"
                     /translation="MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLE
                     TARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNE
                     EQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHF
                     ARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVS
                     PLEDRSTGADFGCQPVMSLTSSLIAVREHKAGEPVGYGGTWVSERDTRLGVVAMGYGD
                     GYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGDPVILWGEGLPVERI
                     AEMTKVSAYELITRLTSRVAMKYVD"
     gene            26134..27327
                     /gene="tyrB"
                     /locus_tag="ACHKBL_001627"
     CDS             26134..27327
                     /gene="tyrB"
                     /locus_tag="ACHKBL_001627"
                     /EC_number="2.6.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HFO5818510.1"
                     /translation="MFQKVDAYAGDPILTLMERFKEDPRSDKVNLSIGLYYNEDGIIP
                     QLKAVADAEARLNAQPHGASLYLPMEGLNSYRHAIAPLLFGADHPVLQQQRVATIQTL
                     GGSGALKVGADFLKRYFPESGVWVSDPTWENHVAIFAGAGFEVSTYPWYDEATNGVRF
                     NDLLATLKTLPARSIVLLHPCCHNPTGADLTNDQWDSVIEILKARELIPFLDIAYQGF
                     GAGMEEDAYAIRAIASAGLPALVSNSFSKIFSLYGERVGGLSVLCEDAEAAGRVLGQL
                     KATVRRNYSSPPNFGAQVVAAVLNDEALKASWLAEVEEMRTRILAMRQELVKVLSTEI
                     PERNFDYLLNQRGMFSYTGLSAAQVDRLREEFGVYLIASGRMCVAGLNTANVQRVAKA
                     FAAVM"
BASE COUNT         6416 a         6998 c         7456 g         6649 t
ORIGIN      
        1 aaaaccccat cactaattcc ccgcgccgta attcgcatgc tttagttgtg tatactcgat
       61 cccgcccgaa atgtttttgg gtaaatctcc attcattcaa tgaagggaaa ttgttatgaa
      121 aaaagttctg tatggcattt ttgccatatc tgcgcttgcg gcgacttctg cgtgggctgc
      181 acctgtacag gtgggcgaag cggcagggtc ggcagcaacg tcggtttcgg cggggagttc
      241 ctccgcgacc agcgtcagca ccgtaagctc ggcggtgggt gtcgcgctcg cggcaagcgg
      301 tggcggtgat ggttctaata ccgggaccac aacaaccacg accaccagta cccagtaata
      361 aagtatgtat ccccaaaata attcgagttg caggaaggcg acaagcgagt gagtccccag
      421 gagcatagat aactatgtga ccggggtgaa cgagtgcggt caccgcatct gcagcttgaa
      481 gtatgaaggg gattaaccat aaccacactc cggtgtggtt attctgcccc tctggagaag
      541 agtcgtgaag cgacctgcac tcattcttat ctgcctgcta ttacaggcct gttcagccac
      601 gactaaagag ctgggcaatt cactgtggga cagtctgttc ggcacgccag gcgtacagct
      661 gacggacgat gatattcaaa atatgcccta cgccagccag tacatgcagc ttaatggcgg
      721 gccgcagtta tttgtggtgc tggccttcgc tgaagacgga caacaaaaat gggtcactca
      781 ggatcaggcc actctcgtca cacaacatgg tcgtctggtg aagactttgc ttggcggcga
      841 caacctgatt gaagtgaata accttgccgc cgacccgctg attaaacccg cacaaattgt
      901 tgatggtgca acatggacgc gcacgatggg ctggatcgag taccagcagg tacgctacgc
      961 caccgcacgc tcagtcttca aatgggatgg caccgatacc gtcaaggtcg gcagcgatga
     1021 aaccccggtt cgcgtgctgg acgaagaagt ctccaccgac caggtgcgct ggcataaccg
     1081 ctactggatc gacagcgaag ggcaaattcg ccagtcggaa cagtatctcg gcgcggatta
     1141 tttcccggtg aaaaccactc tcatcaaggc ggcaaaacaa tgattaaaca aactattgtc
     1201 gcattgattt tgagtgtggg ggcgtcatcg gtcttcgcgg caggaaccgt caaggtgttc
     1261 agcaatggca gcagtgaggc caaaacgcta acgggcgcag agcatttaat cgatctggta
     1321 gggcaacctc gactggcaaa cagctggtgg cctggtgcag tgatcagcga agagctggca
     1381 acggcggcag cattgcggca gcagcaggcg ctactggcac ggctggcaga actggccgca
     1441 gattccagcg ctgacgatgc cgctgcgatt aacgccttac gccagcaaat tcaggcgttg
     1501 aaagtgacgg gcaggcaaaa aatcaatctt gatcccgata tcgtgcgcgt tgccgaacgt
     1561 ggtaacccac cgttgcaggg caactacaca ctgtgggtcg gaccaccacc gtccactgtc
     1621 acgttgttcg ggcttatcag ccgtcctggc aagcagccat tcactccagg tcgcgacgtg
     1681 gcgagctatc tctccgggca gaacctgctt agcggtgcgg atcgcagcta cgcgtgggtg
     1741 gtttacccgg acggacgcac gcaaaaagcg ccggtggctt actggaacaa gcgtcacgta
     1801 gagccgatgc ccggcagcat tatttatgtt ggcctcgcgg actccgtctg gagtgagaca
     1861 cctgatgccc ttaacgccga cattcttcag actctgacgc agcggatacc ccaataatga
     1921 aaaaaagaca tctgcttagc ttactggcgc tgggtattag cacagcttgc tacggcgaaa
     1981 catatcctgc gcccattggt ccgtcgcaat cggatttcgg tggcgtagga ttattacaaa
     2041 cgcccaccgc gcgcatggcg cgggaagggg agctgagtct gaactatcgc gataacgatc
     2101 agtaccgtta ttactcagct tcagtgcaac tcttcccgtg gctggaaaca acgctgcgct
     2161 acaccgacgt gcgcacccgg cagtacagca gcgtcgaagc gttctctggc gatcaaacgt
     2221 ataaagataa agccttcgat ctcaaactgc gtttgtggga agagagttac tggctgccgc
     2281 aagtggcggt tggtgcgcgg gatatcggcg gtacggggct gttcgatgcg gaatatcttg
     2341 tcgccagcaa agcctggggg ccattcgatt ttacgctcgg cctgggctgg ggatatctgg
     2401 gcaccagcgg taatgtgaaa aatccgctct gttcggccag tgacaaatat tgctatcgcg
     2461 ataacagcta caaacaagcg ggatctatcg acggcagcca gatgttccac ggtccggcct
     2521 cactgtttgg cggcgtggaa taccagacgc cctggcaacc gctgcgcctg aaactggagt
     2581 atgaaggcaa taattatcag caggattttg ccggtaagct ggagcaaaaa agtaagttta
     2641 acgtcggggc gatttatcgc gttaccgatt gggccgacgt taaccttagc tatgaacgtg
     2701 gcaacacttt tatgtttggc gtcacgctgc gcaccaactt taacgatctg cgcccgtcct
     2761 acaacgataa cgcccgcccg caatatcaac cgcaaccaca ggatgccatt ttgcagcatt
     2821 cggtggtggc gaatcagtta acgctgttga aatacaacgc cggactggcc gatccgcaga
     2881 tccagacgaa aggcgatacg ctgtatgtca ccggcgagca ggtgaaatat cgtgattcgc
     2941 gtgaagggat catccgtgct aatcggatcg tgatgaacga tctgccggat gggatcaaaa
     3001 cgatccgcat tacggaaaat cgccttaaca tgccgcaggt gacgacggaa accgatgtcg
     3061 ccagcctgaa aaatcatctc gccggagagc cgttgggcca cgaaacgaag ctggcgcaaa
     3121 aacgcgtcga gccagtggtt ccgcagtcca ccgagcaggg ctggtatatc gacaaatcac
     3181 gctttgattt ccatatcgat ccggtgttga accagtcggt cggtggcccg gaaaactttt
     3241 acatgtatca gctgggcgtg atgggaacgg cggatttgtg gctgacggac catctgctga
     3301 ccaccggcag cctgtttgct aaccttgcca acaactacga caagtttaac tacaccaatc
     3361 cgccgcagga ctcgcactta ccgcgcgttc gtacccatgt gcgcgagtat gtgcagaacg
     3421 atgtctatgt gaataacctg caagccaact acttccagca tctgggcaac ggcttctacg
     3481 gtcaggtcta cggaggttat ctcgaaacca tgtttggcgg tgcgggggca gaagtgttgt
     3541 atcgcccgct ggacagcaac tgggcgtttg gtctggatgc caactacgtt aaacagcgcg
     3601 actggcgtag tgcaaaagat atgatgaaat tcaccgacta cagcgtgaaa accggacatc
     3661 tgaccgcata ctggacgcca tcttttgctc aggatgtgtt agtgaaagcg agcgtcgggc
     3721 agtatctggc aggggataaa ggcggcacgc tggagatcgc caaacgcttt gatagcggcg
     3781 tggtggtggg tggctatgcc acgatcacca acgtttcgaa agaggagtac ggcgaagggg
     3841 actttaccaa aggcgtgtat gtctctgtac cgctggatct cttctcgtct ggcccgacac
     3901 gtagccgtgc ggcaattggc tggacgccgc tgacgcgtga cggtggtcag caacttgggc
     3961 gtaagttcca gttgtatgac atgaccagcg accgtagcgt caatttccgc taagtcatgg
     4021 aaaaggtgcc agttttcgca ctcactggca ccctcattct taatggcctg tcgagccatc
     4081 aggaacgcag ttcacccccg gtttgcgatc tggtgtgtaa aggcgaaaac attctgtctt
     4141 cctgtccgtc gatatcggtt gcaggtctgg ttgcctgagc gactgggcca tcattggact
     4201 ggagacattg ctttgcggta ggtcggcttt atcagcagta ccgagcggac cagcataagc
     4261 aggaagaaca gagactgata acatcaaagc agcaaaataa ggcttcattt ttaccacctt
     4321 tatcaggtta cgtttcattt gttccagagg aacattgtcg atttttcgcg cattgctggt
     4381 ggctgggaat cacctgaatg ggtgattttt ttggctgtgt gttttgccag atgagacatt
     4441 gatgggagat gcccacttaa ttgagctgaa caaaacatac acaaaaaata tagatctccg
     4501 tcacattttt gcgttataca ggaagctcgc cactgtgaag gaggtactgc tatgacgtca
     4561 ctctctcgtc cgcgcgtgga gtttatatcc accattttgc agaccgtact caatcttggc
     4621 ctgttgtgcc tcggcctgat tttggttgtc ttcctcggca aagaaacggt gcatctggct
     4681 gatgtgctgt tcgcgccaga acaaaccagt aaatatgagc tggtagaagg gctggtggtt
     4741 tactttctct atttcgaatt tatcgcgctg attgtgaagt actttcagtc cggttttcac
     4801 ttcccgctac gttactttgt ctatattggg atcaccgcca ttgtgcgctt gatcatcgtc
     4861 gatcataaat cgccactgga tgtgttgatc tactcggcag cgatcctgtt actggtgatc
     4921 accctgtggt tgtgtaattc gaaacggctg aagcgggagt aaaaaaaggc gctccgtgga
     4981 gcgccgaata acagtcacaa gttgggataa cgtaagttga gggtgcagcg gcataacatt
     5041 ggcagaacaa catctttaac ctttcacacc acctgccgtc aggccgttga ccagccagcg
     5101 ttgagccagc aagaagacga tggtgatcgg taatgcagac atcacggcag cggcggcaaa
     5161 gtcaccccac aggtagtttt gcgggttgag gtattgctgc atccccacag ccagggtgta
     5221 gctgtttacg tcacgcagta atagcgacgc gaccggaact tcagtaatgg cagcgataaa
     5281 cgacaggatg aacacaaccg ccagaatcgg tactgacagc ggcaacagga caaggcggaa
     5341 ggcctgccac ggtgtcgcac catccagcgc cgccgcttct tccagcgaac tgtcgatggt
     5401 ttcgaaataa cctttgatgg tccagacatg cagcgcaatc ccgcccagat acgcgaaaat
     5461 tacgccgccg tgagtattca ggccaatgaa tggaatgtac tcacccagac gatcaaacaa
     5521 cgcatacaac gcgaccagtg aaagtactgc cgggaacatc tggaaaatca gcattccttt
     5581 cagcagcgtc gctttgcctg gaaagcgcat acgggcgaaa gcgtaggcgc aggtggtgga
     5641 gagcgccaca atgccaatcg cggaaatccc ggcgaccttt accgagttcc acagccatag
     5701 cagtaccgga aacggcggcg gtgtaacgcg accatcagcc tgttccacgc taaaacctaa
     5761 tgccagtttc cagtgatccc aggagatttg ctccgggatc aggctgccgg tcgcaaagtt
     5821 cccctggcgc agtgagatag cgacgaccat cagtagcggg aacataatcg ccgcgataaa
     5881 aagtagcagt agcaggtgag taataaacaa acgtgttttt tgcgatttcg gttggaccat
     5941 tgccattttt gttatctccc ttaatcaaac ttcattcgcg tggctttcag gttcactatc
     6001 gccagcgcac ccaccagcag gaagatcagc gtggcaattg ctgccgccag accgaagtcc
     6061 tgacccccgc cgccttcaaa agcgatgcgg taggtgtagt taacaagcag gtcggtataa
     6121 ccggctggcg tggtcgtgcc aagacgatcc gggccgccgt tggttaacag ttgaatcagc
     6181 acgaagttgt taaagttaaa ggcgaagctg gcgatcatca gcggcgtcag cggtttaatc
     6241 agcagcggca gcgtaatctt aaagaagttc tggaacggac ctgcgccatc cattgctgag
     6301 gcttcataca aatcgtccgg aatcgctttc agcaagccca tgcagaggat catcatgtac
     6361 ggataaccca gccaggtatt gacgataatc agcatcgttc gggcggtggt cggatcgctg
     6421 aaccaggcgg gcttcacgcc aaacagcgcg ctcaacatca tgttgatctc accgaagctc
     6481 tggttaaaca accctttgaa aatcaagatt gaaatgaacg atggcaccgc gtagggcaga
     6541 atcagcagga cgcgatagac cgctttgccg cgcaacgctt cccactgcac cagacacgcc
     6601 agaaccatgc cgaccgccac cgttaaaaag acagtgatca gcgagaacac cacggtccag
     6661 acgaaaatgg cgaggaacgg tttctgaatg ccttcgtcgg tgaagacgcg ggtaaagttt
     6721 ttccagccag tggtcacggt gtaaccgggg cttagctttt catcgcccca gttgccgtcg
     6781 gcggtaatgg actggtaaaa gccaatttgg ttattcggac gatatttcac gccgctctga
     6841 ttattcgtca acgtgccgtc accgtcgagt gtgtagagcg gctgcgtgcc agaaaactgg
     6901 cgcagggagc tcatcatcac tttgttgcca tccggcagaa tggcggtaat gtcactcagc
     6961 gcctgacgat tctgggtaat cacgcgtaga ttcgcgcgtt cgccttcggg ctgggcggtc
     7021 gtttctttca gttgcagttt ttgctcgccg ccaaatttaa aagcgtcgga aaggtaattt
     7081 ttgccggttt cgccgtcgct gagcgccagt tgccactcat cgcccgccgg gtaaagacca
     7141 aagttatagg ttttgcctgc ttgccaggag cgatctaaca acacttcctg tgcacgttca
     7201 aaagtcagct ggttagtgct gctgtagttg gtgaaggcaa tggcgatggt gcagaccaga
     7261 gggaagagga cgaataatcc cattccagcc attcccgggt aaacatagcg ccaggcgtag
     7321 gctttacgat tggcgaaaat atacagcccc gctgaactca atatcagcgt ggtaatggcg
     7381 aacaggtatt ccccttgtgc gtacattaaa acaacaaggt aacccaccag caggccgagc
     7441 agacctaaca ctgaccattt cagcgcgtcg ctttgccacc aatgtttctt tttaatgaca
     7501 tccatggggt tcttcctcat tccaggacgg ataaggcttt cacgccttat ccgacaacaa
     7561 ctgcctgatg cgacgctgac gcgtcttatc aggcctacat acgtttcaat ttcgtaggcc
     7621 ggataaggcg tttacgccgc atccggcatt tcacagcatt acttggtgat acgagtctgc
     7681 gcgtctttca gggcttcatc gacagtctga cgaccgctgg cggcgttgat caccgcagta
     7741 cgcacggcat accagaaagc ggacatctgc gggatgttcg gcataatttc acctttctga
     7801 gcgttatcca tggtggcggc aatacgtgga tctttcgcaa gttcatcctg ataggacttc
     7861 agcgctactg cacccagcgg tttgtcttta ttgaccgctt ccagaccttc gtcagtcagc
     7921 agatagtttt cgaggaactc tttcgccagc tctttgttcg gactggcggc gttaatacct
     7981 gcgctcagca cgccaacgaa cggtttagat ggttgacctt tgaaggtcgg cagtaccgtt
     8041 acaccataat tcactttact ggtgtcgatg ttggaccatg cccacgggcc gttgatagtc
     8101 atcgctgttt cgcctttatt aaaggcagct tctgcgatgg agtaatcggt gtctgcattc
     8161 atgtgtttgt ttttaatcag atcaaccagg aaggttagac ccgctttcgc gccagcgtta
     8221 tccacgccta cgtctttaat gtcgtacttg ccgttttcat acttgaacgc ataacccccg
     8281 tcagccgcaa ttagcggcca ggtgaagtat ggttcttgca ggttgaacat cagcgcgctc
     8341 ttacctttcg ctttcagttc tttatccagc gccgggatct cttcccaggt ttttggcggg
     8401 ttcggtagca gatctttgtt ataaatcagt gataacgctt cgacagcgat cgggtaagca
     8461 atcagcttgc cgttgtaacg tacggcatcc caggtaaacg gatacagctt gtcctggaac
     8521 gctttgtccg gggtgatttc agccaacagg ccagattgag cgtagccgcc aaagcggtcg
     8581 tgcgcccaga agataatgtc agggccatcg ccagttgccg caacctgagg gaatttctct
     8641 tccagtttat ccggatgctc aacggtgact ttaattccgg tatctttctc gaatttctta
     8701 ccgacttcag cgagaccgtt atagccttta tcgccgttaa tccagattac cagtttacct
     8761 tcttcgattt tggcgagagc cgaggcggaa aacatcatcg tcgttaatgc ggataatgcg
     8821 aggatgcgtg cacctgtttt tattttcata atctatggtc cttgttggtg aagtgctcgt
     8881 gaaaacacct aaacggactc tagtttcttt atacggcaac ctctttccat cctccttgcc
     8941 cctacgcccc accgttgctt tgtgtgatct ctgttacaga attggcggta atgtggagat
     9001 gcgcacataa aatcgccatg atttttgcaa gcaacatcac gaaattcctt acatgacctc
     9061 ggtttagttc acagaagctg cgttctcatc ctcccgcctc ctcccccata aaaaagccag
     9121 ggggtggagg atttaagcca tctcctgatg acgcatagtc agcccatcat gaatgttgct
     9181 gtcgatgaca ggttgttaca aagggagaag ggcatggcga gcgtacagct gcaaaatgta
     9241 acgaaagcct ggggcgaggt cgtggtatcg aaagatatca atctcgatat ccatgaaggt
     9301 gaattcgtgg tgtttgtcgg accgtctggc tgcggtaaat cgactttact gcgcatgatt
     9361 gccgggcttg agacgatcac cagcggcgac ctgttcatcg gtgagaaacg gatgaatgac
     9421 actccgcctg cagaacgcgg cgttggtatg gtgtttcagt cttacgcgct ctatccccac
     9481 ctgtcagtag cagaaaacat gtcatttggc ctgaaactgg ctggcgcgaa aaaagaagtg
     9541 attaaccagc gagttaacca ggttgcggaa gtgctgcaac tggcgcattt gctggatcgc
     9601 aaaccgaaag cgctctccgg tggtcagcgt cagcgtgtgg cgattggccg tacgctggta
     9661 gccgagccaa gcgtattttt gctcgatgaa ccgctctcca acctcgatgc tgcactgcgt
     9721 gtgcaaatgc gtatcgaaat ctcccgtctg cataaacgcc tgggccgcac aatgatttac
     9781 gtcacccacg atcaggtcga agcgatgaca ctagccgaca aaatcgtggt gctggacgcc
     9841 ggtcgcgtgg cgcaggttgg gaaaccgctg gagctgtacc actatccggc agaccgtttt
     9901 gtcgccggat ttatcggttc gccaaagatg aacttcctgc cagtaaaagt gaccgccact
     9961 gcaatcgatc aagtgcaggt ggagctgccg atgccaaatc gtcagcaagt ctggctgccg
    10021 gttgaaagcc gtgatgtcca ggttggagcc aatatgtcgc tgggtattcg cccggaacat
    10081 ctactgccga gtgatatcgc tgacgtcatc cttgagggtg aagttcaggt cgtcgagcaa
    10141 ctcggcaacg aaacccaaat ccatatccag atcccttcca ttcgtcaaaa cctggtgtac
    10201 cgccagaacg acgtggtgtt ggtagaagaa ggtgccacat tcgctatcgg cctgccgcca
    10261 gagcgttgcc atctgttccg tgaggatggc actgcatgtc gtcgactgca taaggagccg
    10321 ggcgtttaag caccccacaa aacacacaaa gcctgtcaca ggtgatgtga aaaaagaaaa
    10381 gcaatgactc aggagataga atgatgatta ctctgcgcaa acttcctctg gcggttgccg
    10441 tcgcagcggg cgtaatgtct gctcaggcaa tggctgttga tttccacggc tatgcacgtt
    10501 ccggtattgg ctggacaggt agcggcggtg aacaacagtg tttccagact accggtgctc
    10561 aaagtaaata ccgtcttggc aacgaatgtg aaacttatgc tgaattaaaa ttgggtcagg
    10621 aagtgtggaa agagggcgat aagagcttct atttcgacac taacgtggcc tattccgtcg
    10681 cacaacagaa tgactgggaa gctaccgatc cggccttccg tgaagcaaac gtgcagggta
    10741 aaaacctgat cgaatggctg ccaggttcca ccatctgggc aggtaagcgc ttctaccaac
    10801 gtcatgacgt tcatatgatc gacttctact actgggatat ttctggtcct ggtgccggtc
    10861 tggaaaacat cgatgttggc ttcggtaaac tctctctggc agcaacccgt tcctctgaag
    10921 ctggtggttc ttcctctttc gccagcaaca atatttatga ctataccaac gaaaccgcga
    10981 acgacgtttt cgacgtgcgt ttagcgcaga tggaaatcaa cccgggcggc acattagaac
    11041 tgggtgtcga ctacggtcgt gccaacctgc gtgataacta tcgtctggtt gatggcgcat
    11101 cgaaagacgg ttggttattc actgctgaac atactcagag tgtcctgaag ggctttaaca
    11161 agtttgttgt tcagtacgct actgactcga tgacctcgca gggtaaaggt ctgtcgcagg
    11221 gttctggcgt cgcgtttgat aacgaaaaat ttgcctacaa tatcaacaac aacggtcaca
    11281 tgctgcgtat cctcgaccac ggtgcgatct ccatgggcga caactgggac atgatgtacg
    11341 tgggtatgta ccaggatatc aactgggata acgacaacgg caccaagtgg tggaccgtcg
    11401 gtattcgccc gatgtacaag tggacgccaa tcatgagcac cgtgatggaa atcggctacg
    11461 acaacgtcga atcccagcgc accggcgaca agaacaatca gtacaaaatt acccttgcac
    11521 aacaatggca ggctggcgac agcatctggt cacgcccggc tattcgtgtc ttcgcaacct
    11581 acgccaagtg ggatgagaaa tggggttatg actacaccgg taacgccaat accaacacta
    11641 acttcggcaa agccgttcct gctgatttca acggcggcag cttcggccgt ggtgacagcg
    11701 acgagtggac cttcggtgcc cagatggaaa tctggtggta atagcaaaac ctgggccgga
    11761 taaggtgctt gcgccgcatc cggcaaccaa cgcctgatgc gacgcttgcg cgtcttatca
    11821 ggcctacaac tacaaccgct gccgaaagta ggccggataa ggcgtttacg ccgcatccgg
    11881 cataaaaaca gctttaaggg gcgaaagccc ctctgattat cgggtttagc gcgctattgc
    11941 ctggctaccg ctgagctcca gattttgagg tgaaaacaat gaaaatgaat aaaagtctca
    12001 tcgctctctg tttatcagca gggttgctgg caagcgcacc tggaatcagc cttgccgatg
    12061 ttaactacgt accgcaaaac accagcgacg cgccagccat tccatctgcc gcgctgcaac
    12121 aactcacctg gacaccggtc gatcaatcta aaacccagac cacccaactg gcgactggcg
    12181 gccaacaact gaacgttccc ggcatcagag gtccggttgc tgcgtacagc gtcccggcaa
    12241 acattggcga actgacccta acgctgacca gcgaagtgaa caaacaaact agcgtatttg
    12301 cgccgaacgt gctgattctt gatcagaaca tgaccccatc agccttcttc cccagcagtt
    12361 atttcaccta ccaggaacca ggcgtgatga gtgcagatcg tctggaaggc gttatgcgcc
    12421 tgacaccggc gttggggcag caaaaacttt atgttctggt ctttaccacg gaaaaagacc
    12481 tccagcagac gacccaactg ctcgacccgg ctaaagccta tgccaaaggc gtcggtaact
    12541 cgatcccgga tattcctgac ccggttgctc gtcataccac cgatggctta ctgaaactga
    12601 aagtcaaaac gaactccagt tctagcgtgt tggtcgggcc tctatttggt tcttccgctc
    12661 cagctccggt tacggtaggc aacacggcgg caccagctgt ggctgcaccc gctccggcac
    12721 cggtgaagaa aagcgagccg atgctcaacg acacggaaag ttattttaat accgcgatca
    12781 aaaacgctgt cgcgaaaggt gatgttgata aggcgttaaa actgcttgat gaagctgaac
    12841 gcctggggtc gacatctgcc cgttccacct ttatcagcag tgtaaaaggc aaggggtaat
    12901 tacgccccac agtgctgatt ttgcaacaac tggtgcgtct cctggcgcac ctttttttct
    12961 cctcctgtac tattgttgcg tttttgttgc gcattagttc acttaatttg ctttacatct
    13021 cccgtaaata tttttctgcg atacaatgcc tttacgttat gtaacggaga gttcggcatg
    13081 tcacaccccg cgttaacgca actgcgtgcg ctgcgctatt ttacagagat ccctgccctg
    13141 gagccgcaac tgctcgactg gctgttgctg gaagattcca tgacaaaacg ttttgaacag
    13201 cagggaaaaa cggtgagcgt gacgatgatc cgcgaagggt ttgtcgagca gaatgaaatc
    13261 cccgaagaac tgccactgct gccgaaagag tctcgttact ggttacgtga aattttgtta
    13321 tgtgccgatg gtgaaccgtg gcttgccggt cgtaccgtcg ttcctgtgtc aacgttaagc
    13381 gggccggagc tggcgttaca aaaattaggt aaaacgccgt taggacgcta tctgttcaca
    13441 tcatcgacat taacccggga ctttattgag ataggccgtg atgccggact gtgggggcga
    13501 cgttcccgcc tgcgattaag cggtaaaccg ctgttgctaa cagaactgtt tttaccggcg
    13561 tcaccgttgt actaagagga aaaaaatatg gagtggagtc tgacgcagaa taagctgctg
    13621 gcgtttcatc gcttaatgcg tacggataag ccaattggcg cgttactgct gctctggcca
    13681 acattatggg cgttgtgggt ggcgacaccg ggcgttcccc agctctggat cctggcggtg
    13741 tttgtcgcgg gcgtctggct gatgcgcgct gctggttgcg tggtgaatga ttatgctgac
    13801 cgcaagtttg atggtcatgt taagcgcacg gcgaaccgac cactccccag cggcgcggta
    13861 acggagaaag aggcgcgcgc gctgtttgta gtgctggtac tgatttcatt tttactggtg
    13921 ctgacgctga atacgatgac tattctgttg tcgattgccg cgctggcgct ggcgtgggta
    13981 tacccgttta tgaagcggta tacccatcta ccgcaagtgg tgctgggcgc ggcgtttggc
    14041 tggtcgattc caatggcttt tgctgctgtg agtgagtcgg tgccattgag ttgctggtta
    14101 atgttcctcg ccaatattct ctgggcggtg gcatacgaca cgcagtatgc gatggttgac
    14161 cgtgatgatg atgtgaagat tggcattaaa tccaccgcaa tcctgttcgg ccaatacgat
    14221 aaattgatta ttggtatttt gcagattggc gtactggcgc tgatggcgat tatcggtgag
    14281 ttaaatggct taggctgggg atattactgg tcaattctgg tggctggcgc gctgtttgtt
    14341 tatcaacaaa aactgattgc caaccgcgag cgtgaagcct gctttaaagc atttatgaat
    14401 aataactatg ttggtctggt actgttttta gggctggcaa tgagttactg gcatttctga
    14461 tgatgtaaaa aagccggatg atcatccggc ttccttctgg gttgcctgat gcgctgcgct
    14521 tctcaggcct acacaacata tcgcaattta ttgaatttgc agatcatggt aggccgaata
    14581 aggcgtttgc gccgcatccg gcaattctcc ctgattaccc ttcgctctgc gtcgcactct
    14641 caatcgtcaa acgcacgtct gatgtaatca actccgccag caactgatac accttcatcg
    14701 tttctgccgg ttcggcgtcg ccgctatcgc tgatataccc ttcatcacgc agtgtcagca
    14761 ccagagaact gaacaccgcc ttgtcgaaga actccggcgc gttgatgccg tgcagcacgg
    14821 agagacgttg cgcgacggtg cggctctctt tctccagcgt accgcggttg atcgacgggt
    14881 tggcgctcaa cagccagaag gtgatggcat aacgttgcag cgtttcgcgc gcgcctgcgg
    14941 ccagcagctg tagcgtgcga gaatgcgccg ggttgatatg caactcatca tcttgcaggg
    15001 taatcagccc ctgacgttgc atctcatttg ccagtgcatc aataacgccc ggcaactcgt
    15061 cgcgatccca gcgcaggaac agctccgctt tcagcattgg gtaaagcaca ttgacgtgct
    15121 ccatcaatac gtcgcgggag atgtggcgat gctgggtgac aattgccgcc atcagcgaag
    15181 gcagcaccaa catatgcgca atgttgttgc gatagtaggt catcagcacc gcttgctcgc
    15241 gcggcagaat gatgatgtcg ccgatcgtgt ctttctcgac ttcaaacttg ttcatttgca
    15301 gcgcgtgatc gataagctcg ctggcgctgg ctgaaggaac ggtagagtcc gtggagtaag
    15361 gcacgttgcg catcagatcc aggtagcagt tgagttgctc ggttaactgc tcgcgggtga
    15421 gtgagcgctg acgtgacgcc agaagcgcag tacaacacag gttcatggcg tttgccgcgc
    15481 ctgcgttgtt aatgcgtacc atcagatcgg cagcaatatt attgaccgtc ggcgttaacc
    15541 atgccggacg caccgcttcg atgggatcga tagattcacg ccagtcaggt acatgctggt
    15601 taaggtaggt catcaacggc attggttcac cgaagttgac gtaaccctga ccgagattac
    15661 gcagcttgct taaaccgcgc agcatctgcg gcaggctctc tttctctttc gtcgcaccgc
    15721 gcagttcttt ggcgtaagta cccacttcca tgacgtgctc ataaccgata tagatcggaa
    15781 tcagcgtaat cggacgcgtg ccgccacgca gcatcgcctg aatggtcatc gacagcgtac
    15841 cggttttcgg atccagcaaa cgccctgtac gggaacgacc gccttccacg aaatactcga
    15901 cggaataacc acggctgaac agttcgccga gatactcacg gaaaacggtg gaatagagct
    15961 tattgccttt aaacgtacgg cgaataaaga acgcccccag acggcggaaa atcggcccgg
    16021 caggccagaa attcaggttg atcccggcgg cgatatgcgg cggcaccagc ccctggtgat
    16081 acagcacgta agaaagcagc aggtagtcca tgtggctgcg gtggcaaggc acatacacca
    16141 gctcatgacc gtcgtgggcc agctgacgaa cgcgctcggc gttatggacg ttgatgccct
    16201 ggtaaaggcg gttccaggtg aagcccagaa tacggtcagt caggcggatc atctcgtaag
    16261 agaaattcgc cgcaatctct tccatcagcg caatcgcgtt ctgctgcgct ttttcatggg
    16321 agattttttt gctgcgcgct tcatcttcta ccgctttggc aatggcgcgg gaggcgagca
    16381 gcttattaaa cagatcctga cgagcaggca gacgtgggcc tacggcagcc agacgttgac
    16441 gggcaaagtg catacgcgcc acgcgcgcca gtttctgcgc gatagtttta tccgtgccgt
    16501 gttcatccgc catacggcgc agcgaaactg acggcgagaa acgcacaaaa ctgtcgcgac
    16561 cgagccacag tacagcgaaa aatttctgta cgccgttaag catacgcagc ggcgggttca
    16621 cttcgccttt ttcacgcccc ggcgcgcgac caaacatcac cgatactggc accatctgca
    16681 catccagatt tgggttgcta cggtgcaaat cgagatagtc gtggaacagc ttaatagact
    16741 cttctttcgg cgtgtaatag gtgaacacac gcggcccgcc gtgaatgaac acatagcgcg
    16801 gcagtagcgt gccgtcgatt tccagcggct ctaacgggtc aggcaggtca tgtgccagac
    16861 actgggcgcg caacgtcagc aagtcagctt tcgagttgta cggtaaaacg tacataattg
    16921 gacgagaggt atccagcccc agttccgggg caggatctgc cggaatagac ttgcttttta
    16981 ccaggatgct taatggtaaa ttcagtaatt tgtagtaaat tcgtggccag ccggacataa
    17041 acgatgtaaa gcctctggtt aataatgcaa atgcgcggca aggatagcag aaagtcatgg
    17101 gaaattctgt ggtatccgct catgtttcgc gcggcgttac gcaaacccga atcatcggat
    17161 ttaacggtac actgatattg acgctcataa tgtaaaaagg ttctttcaat ggccaataat
    17221 accactggat tcacccgaat tatcaaagct gctggctatt cctggaaagg tttacgcgct
    17281 gcatggatca acgaagcggc attccgtcag gaaggcgtag cggtattgtt ggcggtggtc
    17341 atcgcctgct ggctggatgt ggacgcaatt acccgcgtgc tgcttatcag ctccgtgatg
    17401 ctggtgatga ttgtggaaat cctcaatagc gccatcgaag cagtggttga ccgaattggc
    17461 tctgaatacc atgagctttc cggacgcgct aaagatatgg gatccgccgc ggttctgatt
    17521 gcgattatcg tcgccgtgat tacctggtgc atcctgttat ggtcgcattt tggataaccc
    17581 ttccagattt cgataaatct ctggtttatt gtgcagttta tggttccaaa atcgcctttt
    17641 gctgtatata ctcacagcat aactgtatat acacccaggg ggcggaatga aagcgttaac
    17701 ggccaggcaa caagaggtgt ttgatctcat ccgtgatcac atcagccaga caggtatgcc
    17761 gccgacgcgt gcggaaatcg cgcagcgttt ggggttccgt tccccaaacg cggctgaaga
    17821 acatctgaag gcgctggcac gcaaaggcgt tattgaaatt gtttccggcg catcacgcgg
    17881 gattcgtctg ttgcaggaag aggaagaagg gttgccgctg gtaggtcgtg tggctgccgg
    17941 tgaaccgctt ctggcgcaac agcatattga aggtcattat caggtcgatc cttccttatt
    18001 caagccgaat gctgatttcc tgctgcgcgt cagcgggatg tcgatgaaag atatcggcat
    18061 tatggatggc gacttgctgg cagtgcataa aactcaggat gtacgtaacg gtcaggtcgt
    18121 tgtcgcacgt attgatgacg aggttaccgt taagcgcctg aaaaaacagg gcaataaagt
    18181 cgaactgttg ccagaaaata gcgagtttaa accaattgtc gttgaccttc gtcagcagag
    18241 cttcaccatt gaagggctgg cggttggagt tattcgcaac ggcgactggc tgtaacatat
    18301 ctctgagacc gcgatgccgc ctggcgtcgc ggtttgtttt tcatctctct tcatcaggct
    18361 tgtctgcatg gcattcctca cttcatctga taaagcactc tggcatctcg ccttacccat
    18421 gattttctcc aatatcaccg ttccgttgct gggactggtc gatacggcgg taattggtca
    18481 tcttgatagt ccggtttatt tgggcggcgt ggcggttggt gcaacggcga ccagctttct
    18541 ctttatgctg ttgctgtttt tacgcatgag caccaccggg ctgactgcgc aggcttatgg
    18601 tgccaaaaat cctcaggcat tagcccgtgc gctggtgcaa ccgttgctgt tggcgttggg
    18661 ggctggggcg ttaattgcgc tgctgcgtac gccgattatc gatctggcgc tgcatattgt
    18721 tggcggcagc gaagcggtgc tagaacaggc gcgacgcttt cttgaaatcc gctggttaag
    18781 tgcaccggcg tcgctggcga atctggtatt acttggttgg ttactcggtg tgcaatatgc
    18841 ccgtgcgcca gtaattttgt tagtggtcgg caatatcctc aacattgtgc tggatgtctg
    18901 gctggtgatg gggctgcata tgaacgtgca gggcgcggcg ctggcgacgg ttattgcgga
    18961 atatgcaaca ttgctgattg gtctgctaat ggtgcgtaaa atcctcaaac tacgcggaat
    19021 ctccggcgaa atgctgaaaa ctgcctggcg aggaaacttc cgtcacttgc tggcgcttaa
    19081 ccgcgatatc atgctgcgct cgctgttgtt gcaactctgt ttcggcgcga tcaccgtact
    19141 tggcgcgcga ctggggagtg acattatcgc tgttaacgcg gttctgatga cgttactcac
    19201 ctttaccgcc tatgcgctgg atggttttgc ctacgcggtt gaagcgcatt ccggtcaggc
    19261 gtacggtgcg cgcgatggta gccagttact ggatgtctgg cgggcagcgt gccgccagtc
    19321 ggggatcgta gcgttactgt tttcggtggt ttatttgctg gcaggggaac acatcattgc
    19381 gttgctgacg tcgttaaccc agattcagca gctggctgac cgctatctta tctggcaggt
    19441 gattttgccg ttggtcggcg tctggtgtta tctgctcgac ggcatgttta taggtgcaac
    19501 gcgcgccgcc gaaatgcgta acagtatggc ggtggccgcc gcaggttttg cgctgacgct
    19561 ccttacgctg ccgtggctgg ggaatcatgg tttgtggctg gcattaaccg tctttctggc
    19621 tttgcgcggg ctttctctgg cggctatctg gcggcgtcac tggcgcaacg gtacctggtt
    19681 tgccgcaacg tgacggttaa aaattctgaa taaataatcc taagccaaat tgctgactac
    19741 acttaatctc acgttcagaa gaaaagtgaa cgtactctca ttcacaacct aacgatgagg
    19801 tcttgattat gaataaagat gaagccggcg gtaactggaa acagtttaaa ggtaaagtga
    19861 aagagcaatg gggcaaactg accgatgatg atatgacgat cattgaaggt aaacgtgatc
    19921 aactggtcgg taaaatccag gaacgttatg gttatcagaa agatcaggca gaaaaagagg
    19981 ttgatagttg ggaaaaacgc catgattacc gctggtaatt aatccctcct gcccgacatg
    20041 tacaaggatg tacacccttt tagcgaggtt tctttttcac ctgaatagag tggtcatgat
    20101 ggcactgttc gggatgacga cacgcttcta cttctacaca tgctgaacat aatccatgtg
    20161 cttcaataac attatgccgc agggcaaaac ccatttttgc cgccagcgta tgcatgatgt
    20221 cttccacgcc ttcagcacat tcttctttca ccgcgccgca gcgatcgcaa ataaacatgg
    20281 ctgaagtatg ggtgggctga tcgaacagat gacagagcac ataactgttg gtggattcca
    20341 ccttatgcac aaaaccttgt tcaagcagaa actccagcgc gcgataaacc gttggcggct
    20401 tggcttgcgg ttcagcttcg cgcagcaaat caagcagatc ataagcgctg atagcgccat
    20461 cctgcagact catcaggcgc aatacttcca ggcgctgtgg ggtcaggcgc acattacgct
    20521 gtgcgcagat tttttcagcc tgcgctaata actcctgcgt tgtggtcttt tccattgggc
    20581 acctcgacag tggggttaag aatgtcctta ctttaccata gttctcgctt aaacgccgag
    20641 aacagggtgt catggctgtc acattgcctg atcgcatatt cgttatgcta tagtagcgcc
    20701 cccaatttac cagatgctta aaaagtcgcc atgccagcag aatcacacac tgtttatccc
    20761 gcttaccgtt tctcgattgc cccgatgctt gactggacgg acagacattg ccgctatttc
    20821 ttgcgtctgc tttcccgcaa tacgttgctg tataccgaaa tggtgactac aggggcgatt
    20881 attcacggta aaggtgatta cctggcgtac agtgaagaag aacatccggt agcgttgcaa
    20941 ctcgggggta gcgatccggc ggcgctggca cagtgtgcga agctggcaga agcgcgcgga
    21001 tatgatgaga tcaacctgaa tgtcggctgc ccgtctgacc gggtgcagaa cggcatgttt
    21061 ggtgcgtgtc tgatgggtaa tgcgcagctg gttgccgact gcgtgaaagc gatgcgcgat
    21121 gtggtgtcga ttccggtgac ggtgaaaacg cgtattggca tcgatgatca ggacagctat
    21181 gaatttctct gcgatttcat caacaccgtt tccggcaaag gtgagtgtga gatgttcatc
    21241 atccacgcac gtaaagcctg gctttcgggg ttaagcccga aagaaaaccg tgaaatcccg
    21301 ccgctcgatt atccgcgtgt gtatcaactg aagcgtgact ttccgcatct gacgatgtcg
    21361 attaacggtg gcatcaagtc gctggaagag gctaaagcgc atttgcaaca tatggatggc
    21421 gtgatggtcg ggcgcgaagc gtatcagaat ccaggcattc tggcggcggt agaccgagag
    21481 atctttggtt cttcggatac cgatgtcgat ccggtggcgg tggtgcgtgc catgtatccg
    21541 tacattgagc gtgaactcag ccaggggacg tatctcggcc atattacccg gcatatgctg
    21601 ggcttgttcc agggtattcc tggcgcgcgg cagtggcggc gttatttaag tgaaaatgcc
    21661 cataaagcgg gtgctgacat taacgtgctg gaacacgcgc tcaaactggt ggcggataag
    21721 cgttaacttt tcaccaaaaa gtagtcaaat tcaccacgcc ctgcgcaccg tcgcggggcg
    21781 ttttgctgtt aaatcaatag attatttttg gcatgattct tgtaatgcct gcaagagatt
    21841 tcatatttgg gagagcatca tgctggaact actttttgtg attggctttt ttgtcatgct
    21901 gatggtcacc ggcgtttcgt tgctgggcat tatcgccgcg ctggttgtgg cgacggccat
    21961 tatgttcctc ggcggtatgc tggcattgat gattaagttg ctgccgtggt tactcctggc
    22021 gattgcggtg gtgtgggtta ttaaggcgat taaagcacca aaagtgccga aatatcagcg
    22081 ttatgaccgc tggcgttact aagggattgt gcggatgatc acaacctaag gttttatcct
    22141 tagaacaaaa taggaattga taatcaaatc tgtcactatt gcgcctctaa cagattcatc
    22201 gtgctgtacc ctacatacag ccgaactata aaaagaaagg gcttcccaga tggaagccct
    22261 atttctttta tggaatcaac aggctggaac cttgcgtcgc ccggctttcc agaatctcat
    22321 gcgcacgctg tgcatcctta agcggatatt tctgctgctc ggcgacatcg accttaatca
    22381 caccgctggc aatcaaagag aacagttcat tactggcctc ggttaattcc tcccgtgtgg
    22441 tgatatagcc ttgtagggaa gggcgtgtca catacaacga gcctttctga ttgagaatgc
    22501 ctaagttcac accggtaacc gcacctgatg agttgccaaa gctcaccatt aagccgcggc
    22561 gttgcaggca atccagcgac cgttcccagg tgtctctgcc cacggaatcg tacaccacgc
    22621 gcactttctt gccaccggtg atctctttta accgctcgac cagattctct tcacgatagt
    22681 taataacctg ccatgcgccc gcttttagcg cactctgcgc tttttgcgcg gttcctacgg
    22741 tgccgataag tttcgcgcca agggcttttg cccactggca ggcaatcaag ccaacgccgc
    22801 cagccgctgc gtggaacagg aactgctcat cgggtttaat ttcataggtt ttgcgcagca
    22861 gataataaac cgttaagcct ttcaggaagg atgccgcagc ttgctcaaaa gaaattgccg
    22921 caggcagaat cgccgcttta tccgcattaa tgttatgcac agagctgtaa gcgcctaatg
    22981 ccgattgtgc atagactacg cgatcgcctg ccttaatatg ctttacacca ctgccgactt
    23041 tactcacgat gcctgccgct tcggtgccta atccgctggg tagcgatggc ggcgggtaaa
    23101 ggccgctgcg gatatacgtg tcgataaaat tgatgccgat ggctttattt tcgacctgga
    23161 tttcattctc cgccggatcg gcaggagtga actctacggc ttgaagtact tccgggccac
    23221 cgtgcttgtg aaattcaatt cgtgttgcca tgtgtcctcc agaacgtatc gtcagggtct
    23281 gcttcatatg gtaaagtttc gacccattct ttatctcggt aactccattc actatggcag
    23341 gaaataaacc cttcaacaaa cagcaggctg aaccccgcga acgcgatcca caagttgccg
    23401 ggctgaaagt gcctccgcac tcgatcgaag cggagcagtc ggtgttgggc ggtttaatgc
    23461 tggataacga acgctgggat gatgtagctg agcgtgtggt ggcagatgat ttttacaccc
    23521 gcccacaccg tcatatcttt actgaaatgg cgcgtttgca ggaaagcggt agtcctatcg
    23581 atctgattac ccttgcggaa tcgctggaac gccaggggca actcgatagc gtcggtggtt
    23641 ttgcttatct ggcagagctg tcaaaaaata cgccaagtgc ggcgaacatc agtgcttatg
    23701 ctgacatcgt gcgtgaacgt gccgttgttc gcgagatgat ttcggttgcg aatgagattg
    23761 ccgaagccgg ttttgatccg caggggcgca ccagcgaaga tctgctggac ctggctgaat
    23821 cccgcgtctt taaaattgcc gaaagtcgtg caaacaaaga cgaagggccg aagaacatcg
    23881 ctgatgtgct cgacgccacg gtggcgcgta ttgagcagtt gtttcagcag ccacacgatg
    23941 gcgttaccgg ggtaaacacc ggttatgacg atctcaacaa aaaaaccgct ggcttgcagc
    24001 cgtcggattt gatcatcgtc gccgcgcgtc cgtcgatggg taaaacaaca tttgcgatga
    24061 acctcgtcga aaacgcggcg atgttgcagg ataaaccagt acttatcttc tcgctggaga
    24121 tgccatcaga acagattatg atgcgttctc tggcgtcgct gtcgcgcgtt gaccagacta
    24181 aaatccgtac cgggcagctc gatgatgaag actgggcgcg catttccggc accatgggta
    24241 ttttgctcga aaaacgcaat atctatatcg atgactcctc cggcctgacg ccaacggaag
    24301 tgcgttcccg cgcgcgccgt attgcccgtg aacacggcgg catcgggctt atcatgatcg
    24361 actacctgca actgatgcgc gtaccggcgc tttccgataa ccgtacgctg gaaattgcag
    24421 aaatctcccg ctcgctgaaa gcactggcga aagaattgaa cgtgccggtg gtggcgctgt
    24481 cccagttgaa ccgttctctg gaacaacgtg ccgacaaacg cccggtcaac tccgacctgc
    24541 gtgaatctgg ctctatcgag caggatgcgg acttgatcat gtttatctat cgtgatgagg
    24601 tgtatcacga aaacagtgat ttaaaaggca tcgcggaaat tattatcggt aaacaacgta
    24661 acggcccaat cgggacggta cgcctgacct ttaacggtca atggtcgcgc ttcgacaact
    24721 atgcggggcc gcagtacgac gacgaataat aattatttta tgaattaggt aattaaagca
    24781 aacacttatc aaggaacaca aatgcaagcg gcaactgttg tgattaaccg ccgcgctctg
    24841 cgacacaacc tgcaacgtct tcgtgaactg gctcctgcca gtaaaatggt tgcggtggtg
    24901 aaagcgaacg cttatggtca cggtcttctt gagaccgcgc gaacgctccc cgatgctgac
    24961 gcctttggcg tagcccgtct cgaagaagct ctgcgactgc gtgcgggggg aatcaccaaa
    25021 cctgtactgt tactcgaagg cttttttgat gccagagatc tgccgacgat ttctgcgcaa
    25081 cattttcata ccgccgtgca taacgaagaa cagctggctg cgctggaaga ggctagcctg
    25141 gacgagccgg ttaccgtctg gatgaaactc gataccggta tgcaccgtct gggcgtaagg
    25201 ccggaacagg ctgaggcgtt ttatcatcgc ctgacccagt gcaaaaacgt tcgtcagccg
    25261 gtgaatatcg tcagccattt tgcgcgcgcg gatgaaccaa aatgcggcgc aaccgagaaa
    25321 caactcgcta tctttaatac cttttgtgaa ggcaaaccag gtcaacgttc cattgccgcg
    25381 tcgggtggca ttctgctgtg gccacagtcg cattttgact gggtgcgccc gggcatcatt
    25441 ctttatggcg tctcgccgct ggaagatcgt tccaccggtg ccgattttgg ctgtcagccg
    25501 gtaatgtcat tgacctccag cctgattgct gtgcgtgagc ataaagccgg agagcctgtc
    25561 ggttatggtg gaacctgggt aagcgaacgt gatacccgcc ttggcgtagt cgcgatgggc
    25621 tatggcgatg gttatccgcg cgccgcgccg tccggtacgc cagtgctggt gaacggtcgc
    25681 gaagtgccga ttgtcgggcg cgtcgcgatg gatatgatct gcgtagactt aggtccacag
    25741 gcgcaggaca aagccgggga cccggtcatt ttatggggcg aaggtttgcc cgtagaacgt
    25801 atcgctgaaa tgacaaaagt gagcgcttac gaacttatta cgcgcctgac ttcaagggtc
    25861 gcgatgaaat acgtggatta atcgttctgt aatatttgat tgtctgtgcc ggatgcggcg
    25921 tgaatgcctt atccggccaa taaaatctta ccaattcaat aagttgatat tctttcatgc
    25981 tcttataaag gtcgtgcatc tggcggatgt acgtttgtca tgagtctcac tctgttgcta
    26041 attgccattc gcccctgaac atcctctcga tcttcgcctt cttccggttt attgtgtttt
    26101 aaccacctgc ccgtaaacct ggagaaccat cgcgtgtttc aaaaagttga cgcctacgct
    26161 ggcgacccga ttcttacgct tatggagcgt tttaaagaag accctcgcag cgacaaagtg
    26221 aatttaagta tcggtctgta ctacaacgaa gacggaatta ttccacaact gaaagccgta
    26281 gcggatgcgg aagcgcgcct gaatgcgcag cctcatggtg cttcgcttta tttaccgatg
    26341 gaagggctta acagctatcg ccatgccatt gcgccgctgc tgtttggtgc ggaccatccg
    26401 gtactgcaac aacagcgcgt agcaaccatt caaacccttg gcggctcagg ggcattgaaa
    26461 gtgggtgcag atttcctgaa acgctacttc ccggaatcag gcgtctgggt cagcgatcct
    26521 acctgggaaa accacgtagc aatattcgcc ggggctggat tcgaagtgag cacttacccc
    26581 tggtatgacg aagcgactaa cggcgtgcgc tttaatgacc tgttggcgac gctgaaaaca
    26641 ttacctgccc gcagtattgt gttgctgcat ccatgttgcc acaacccaac gggtgccgat
    26701 ctcactaatg accagtggga ttccgtgatt gaaattctca aagcccgcga gcttattcca
    26761 ttcctcgata ttgcctatca aggatttggt gccggtatgg aagaggatgc ctacgccatt
    26821 cgcgccattg ccagcgctgg attacccgct ctggtgagca attcgttctc gaaaattttc
    26881 tccctttacg gcgagcgcgt cggcggactt tctgttctgt gtgaagatgc cgaagccgca
    26941 ggccgcgtac tggggcaatt gaaagcaaca gttcgccgca actactccag cccgccgaat
    27001 tttggtgcgc aggtggtggc tgcggtgctg aatgacgagg cattgaaagc cagctggctg
    27061 gcggaagtag aagagatgcg tactcgcatt ctggcgatgc gtcaggaact ggtgaaggta
    27121 ttgagcacag agataccaga acgcaatttc gattatctgc ttaatcagcg cggtatgttc
    27181 agttataccg gtttaagtgc cgctcaggtt gaccgactac gtgaagaatt tggtgtctat
    27241 ctcatcgcca gcggtcgcat gtgtgtcgcc gggttaaata cggcaaatgt acaacgtgtg
    27301 gcaaaggcgt ttgctgcggt gatgtaatgc aggaaagcag gctggagtta tccagcctgc
    27361 agtgaaatta aactgtcgtc gctttcactc ttgctttata gatgattttt ttgatgccat
    27421 cgttctacgt gagaggtaat aaaacgttgt tagttctaac ttgttaagtt tatcccaatg
    27481 ttctggagta cttttccctg ttttaccgtg tggaataat
//
LOCUS       DAZFLD010000046        27503 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.046, whole genome shotgun sequence.
ACCESSION   DAZFLD010000046 DAZFLD010000000
VERSION     DAZFLD010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27503
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.046"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            70..327
                     /gene="bhsA"
                     /locus_tag="ACHKBL_001628"
                     /gene_synonym="ycfR"
     CDS             70..327
                     /gene="bhsA"
                     /locus_tag="ACHKBL_001628"
                     /gene_synonym="ycfR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple stress resistance protein BhsA"
                     /protein_id="HFO5818511.1"
                     /translation="MKNVKTLIAAAILSSMSFASFAAVEVQSTPEGQQKVGTISANAG
                     TNLGSLEEQLAQKADEMGAKSFRITSVTGPNTLHGTAVIYK"
     gene            complement(410..1372)
                     /gene="ldtC"
                     /locus_tag="ACHKBL_001629"
     CDS             complement(410..1372)
                     /gene="ldtC"
                     /locus_tag="ACHKBL_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtC"
                     /protein_id="HFO5818512.1"
                     /translation="MMIKTRFSRWLTFFTFAAAVALALPAKANTWPLPPAGSRLVGEN
                     KFHVVENDGGSLEAIAKKYNVGFLALLQANPGVDPYVPRAGSVLTIPLQTLLPDAPRE
                     GIVINIAELRLYYYPPGKNSVTVYPIGIGQLGGDTLTPTMVTTVSDKRANPTWTPTAN
                     IRARYKAQGIELPAVVPAGPDNPMGHHAIRLAAYGGVYLLHGTNADFGIGMRVSSGCI
                     RLRDDDIKTLFSQVTPGTKVNIINTPIKVSAEPNGARLVEVHQPLSEKIDDDPQLLPI
                     TLNSAMQSFKDAAQTDAEVMQHVMDVRSGMPVDVRRHQVSPQTL"
     gene            complement(1516..4962)
                     /gene="mfd"
                     /locus_tag="ACHKBL_001630"
     CDS             complement(1516..4962)
                     /gene="mfd"
                     /locus_tag="ACHKBL_001630"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HFO5818513.1"
                     /translation="MPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVL
                     IAPDMQNALRLHDEISQFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPTMQ
                     RGVLIVPVNTLMQRVCPHSFLHGHALVMKKGQRLSRDALRTQLDSAGYRHVDQVMEHG
                     EYATRGALLDLFPMGSELPYRLDFFDDEIDSLRVFDVDSQRTLEEVEEINLLPAHEFP
                     TDKAAIELFRSQWRDTFEVKRDPEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYF
                     PANTLLVNTGDLENSAERFQADTLARFENRGVDPMRPLLPPQSLWLRVDELFSELKNW
                     PRVQLKTEHLPTKAANANLGFQKLPDLAVQAQQKAPLDALRKFLESFDGPVVFSVESE
                     GRREALGELLARIKIAPQRIMRLDEASDRGRYLMIGAAEHGFVDTVRNLALICESDLL
                     GERVARRRQDSRRAINPDTLIRNLAELHIGQPVVHLEHGVGRYAGMTTLEAGGITGEY
                     LMLTYANDAKLYVPVSSLHLISRYAGGAEENAPLHKLGGDAWSRARQKAAEKVRDVAA
                     ELLDIYAQRAAKEGFAFKHDREQYQLFCDSFPFETTPDQAQAINAVLSDMCQPLAMDR
                     LVCGDVGFGKTEVAMRAAFLAVDNHKQVAVLVPTTLLAQQHYDNFRDRFANWPVRIEM
                     ISRFRSAKEQTQILAEVAEGKIDILIGTHKLLQSDVKFKDLGLLIVDEEHRFGVRHKE
                     RIKAMRANVDILTLTATPIPRTLNMAMSGMRDLSIIATPPARRLAVKTFVREYDSLVV
                     REAILREILRGGQVYYLYNDVENIQKAAERLAELVPEARIAIGHGQMRERELERVMND
                     FHHQRFNVLVCTTIIETGIDIPTANTIIIERADHFGLAQLHQLRGRVGRSHHQAYAWL
                     LTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRGAGELLGEEQSGSMETIGF
                     SLYMELLENAVDALKAGREPSLEDLTSQQTEVELRMPSLLPDDFIPDVNTRLSFYKRI
                     ASAKTENELEEIKVELIDRFGLLPDPARTLLDIARLRQQAQKLGIRKLEGNEKGGVIE
                     FAEKNHVNPAWLIGLLQKQPQHYRLDGPTRLKFIQDLSERKTRIEWVRQFMRELEENA
                     IA"
     gene            complement(5090..6164)
                     /locus_tag="ACHKBL_001631"
                     /pseudo
     CDS             complement(5090..6164)
                     /locus_tag="ACHKBL_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415633.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
     gene            6426..7625
                     /gene="lolC"
                     /locus_tag="ACHKBL_001632"
     CDS             6426..7625
                     /gene="lolC"
                     /locus_tag="ACHKBL_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolC"
                     /protein_id="HFO5818514.1"
                     /translation="MYQPVALFIGLRYMRGRAADRFGRFVSWLSTIGITLGVMALVTV
                     LSVMNGFERELQNNILGLMPQAILSSEHGSLNPQQLPETAVKLDGVNRVAPITTGDVV
                     LQSARSVAVGVMLGIDPAQKDPLTPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGD
                     QIRVMVPSASQFTPMGRIPSQRLFNVIGTFAANSEVDGYEMLVNIEDASRLMRYPAGN
                     ITGWRLWLDEPLKVDSLSQQKLPEGSKWQDWRDRKGELFQAVRMEKNMMGLLLSLIVA
                     VAAFNIITSLGLMVMEKQGEVAILQTQGLTPRQIMMVFMVQGASAGIIGAILGAALGA
                     LLASQLNNLMPIIGVLLDGAALPVAIEPLQVIVIALVAMAIALLSTLYPSWRAAATQP
                     AEALRYE"
     gene            7618..8319
                     /gene="lolD"
                     /locus_tag="ACHKBL_001633"
     CDS             7618..8319
                     /gene="lolD"
                     /locus_tag="ACHKBL_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HFO5818515.1"
                     /translation="MNKILLQCDNLCKRYQEGSVQTDVLHNVSFSVGEGEMMAIVGSS
                     GSGKSTLLHLLGGLDTPTSGDVIFNGQPMSKLSSAAKAELRNQKLGFIYQFHHLLPDF
                     TALENVAMPLLIGKKKPAEINSRALEMLKAVGLEHRANHRPSELSGGERQRVAIARAL
                     VNNPRLVLADEPTGNLDARNADSIFQLLGELNRLQGTAFLVVTHDLQLAKRMSRQLEM
                     RDGRLTAELSLMGAE"
     gene            8319..9563
                     /gene="lolE"
                     /locus_tag="ACHKBL_001634"
     CDS             8319..9563
                     /gene="lolE"
                     /locus_tag="ACHKBL_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HFO5818516.1"
                     /translation="MAMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVG
                     LSAMNGFERELNNRILAVVPHGEIEAVDQPWTNWQEALDNVQKVPGIAAAAPYINFTG
                     LVESGANLRAIQVKGVNPQQEQRLSALPSFVQGDAWRNFKAGEQQIIIGKGVADALKV
                     KQGDWVSIMIPNSNPEHKLMQPKRVRLHIAGILQLSGQLDHSFAMIPLADAQQYLDMG
                     SSVSGIALKMTDVFNANKLVRDAGEVTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAM
                     VLVIGVACFNIVSTLVMAVKDKSGDIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCG
                     VIIGVVVSLQLTPIIEWIEKLIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSL
                     LASWYPARRASNIDPARVLSGQ"
     gene            9592..10503
                     /gene="nagK"
                     /locus_tag="ACHKBL_001635"
     CDS             9592..10503
                     /gene="nagK"
                     /locus_tag="ACHKBL_001635"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045127 - N-acetylglucosamine kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HFO5818517.1"
                     /translation="MYYGFDIGGTKIALGVFDSGRQLQWEKRVPTPRDSYDAFLDAVC
                     ELVAEADRRFGCKGSVGIGIPGMPETEDGTLYAANVPAASGKPLRADLSARLDRDVRL
                     DNDANCFALSEAWDDEFTQYPLVMGLILGTGVGGGLIFNGKPITGKSYITGEFGHMRL
                     PVDALTMMGLDFPLRRCGCGQHGCIENYLSGRGFAWLYQHYYHQPLQAPEIIALYDQG
                     DEQARAHVERYLDLLAVCLGNILTIVDPDLVVIGGGLSNFPAITTQLADRLPRHLLPV
                     ARVPRIERARHGDAGGMRGAAFLHLTD"
     gene            10519..11340
                     /gene="cobB"
                     /locus_tag="ACHKBL_001636"
     CDS             10519..11340
                     /gene="cobB"
                     /locus_tag="ACHKBL_001636"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD+-dependent deacetylase"
                     /protein_id="HFO5818518.1"
                     /translation="MLSRRGHRLSRFRKNKRRLRERLRQRIFFRDKVVPEAMEKPRVL
                     VLTGAGISAESGIRTFRAADGLWEEHRVEDVATPEGFDRDPELVQTFYNARRRQLQQP
                     EIQPNAAHLALAKLQDALGDRFLLVTQNIDNLHERAGNTNVIHMHGELLKVRCSQSGQ
                     VLDWTGDVTPEDKCHCCQFPAPLRPHVVWFGEMPLGMDEIYMALSMADIFIAIGTSGH
                     VYPAAGFVHEAKLHGAHTVELNLEPSQVGNEFAEKYYGPASQVVPEFVEKLLKGL"
     gene            complement(11466..12251)
                     /locus_tag="ACHKBL_001637"
     CDS             complement(11466..12251)
                     /locus_tag="ACHKBL_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HFO5818519.1"
                     /translation="MKKIIILLSLLILLPLTVTSKPLIPIMKTLFTDVTGTVPDAEEI
                     ARKAELFRQQTGIAPFIVVVPGVNEASFRQNGKAMLAHAASSMSNVKGSVLLLFTTRE
                     PRLIMITNGQVESSMDDKHLGLLVENHTLAYLHADLWYQGINNALAVLQAQILKQPTP
                     PLTYYPHPGQQQENDLPGSTTTLGLFAWAVAFIVFAAFFNYTTRLYYALKFAVAMAVA
                     NMGYQALCLYIDNSFAITRISPLWAGLIGVCTFIAALLWTSKR"
     gene            complement(12248..12709)
                     /locus_tag="ACHKBL_001638"
     CDS             complement(12248..12709)
                     /locus_tag="ACHKBL_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3592 domain-containing protein"
                     /protein_id="HFO5818520.1"
                     /translation="MSQDSRVFFRIFFGIGLVLILISVVIFYNQFTYSKDAIHTEGVI
                     VDTVWHSSHSHRTGKNGSWYPVVAFRPTPDYTLIFNSSIGSDFYEDSEGDKVNVYYSP
                     GHPEKAEINNPWVNFFKWGFIGIMGVIFISVGLLISMPYSKKTRRKRKSRP"
     gene            complement(12767..13813)
                     /gene="potD"
                     /locus_tag="ACHKBL_001639"
     CDS             complement(12767..13813)
                     /gene="potD"
                     /locus_tag="ACHKBL_001639"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotD"
                     /protein_id="HFO5818521.1"
                     /translation="MKKWSRHLLAAGALALGMSAAHADDNNTLYFYNWTEYVPPGLLE
                     QFTKETGIKVIYSTYESNETMYAKLKTYKDGAYDLVVPSTYYVDKMRKEGMIQKIDKS
                     KLSNFSNLDPDMLNKPFDPNNDYSIPYIWGATAIGVNGDAVDPKSVTSWADLWKPEYK
                     GSLLLTDDAREVFQMALRKLGYSGNTTDPKEIEAAYNELKKLMPNVAAFNSDNPANPY
                     MEGEVNLGMIWNGSAFVARQAGTPIDVVWPKEGGIFWMDSLAIPANAKNKEGALKLIN
                     FLLRPDVAKQVAETIGYPTPNLAARKLLSPEVANDKTLYPDAETIKNGEWQNDVGAAS
                     SIYEEYYQKLKAGR"
     gene            complement(13810..14604)
                     /gene="potC"
                     /locus_tag="ACHKBL_001640"
     CDS             complement(13810..14604)
                     /gene="potC"
                     /locus_tag="ACHKBL_001640"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HFO5818522.1"
                     /translation="MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTT
                     KWYSLLMNNDSLLQAAQHSLTMAVFSATFATLIGSLTAVALYRYRFRGKPFVSGMLFV
                     VMMSPDIVMAISLLVLFMLLGIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLE
                     AAKDLGASEFTILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKI
                     YSMVKVGVSPEVNALATILLVLSLVMVIASQLIARDKTKGNTGDVK"
     gene            complement(14601..15458)
                     /gene="potB"
                     /locus_tag="ACHKBL_001641"
     CDS             complement(14601..15458)
                     /gene="potB"
                     /locus_tag="ACHKBL_001641"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HFO5818523.1"
                     /translation="MKNTSKFQNVVIVTIVGWLVLFVFLPNLMIIGTSFLTRDDASFV
                     KMVFTLDNYTRLLDPLYFEVLLHSLNMALIATLACLVLGYPFAWFLAKLPHKVRPLLL
                     FLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYI
                     LLPFMVMPLYSSIEKLDKPLLEAARDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPA
                     MGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATSITLTIVMGLMLLVYWRASR
                     LLNKKVELE"
     gene            complement(15442..16578)
                     /gene="potA"
                     /locus_tag="ACHKBL_001642"
     CDS             complement(15442..16578)
                     /gene="potA"
                     /locus_tag="ACHKBL_001642"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HFO5818524.1"
                     /translation="MGQSKKLNKQPSSLSPLVQLAGIRKCFDGKEVIPQLDLTINNGE
                     FLTLLGPSGCGKTTVLRLIAGLETVDSGRIMLDNEDITHVPAENRYVNTVFQSYALFP
                     HMTVFENVAFGLRMQKTPADEITPRVMEALRMVQLETFAQRKPHQLSGGQQQRVAIAR
                     AVVNKPRLLLLDESLSALDYKLRKQMQNELKALQRKLGITFVFVTHDQEEALTMSDRI
                     VVMRDGRIEQDGTPREIYEEPKNLFVAGFIGEINMFNATVIERLDEQRVRANVEGREC
                     NIYVNFTVEPGQKLHVLLRPEDLRVEEINDDNHAEGLIGYVRERNYKGMTLESVVELE
                     NGKMVMVSEFFNEDDPDFDHSLDQKMAINWVESWEVVLADEEHK"
     gene            16828..18057
                     /gene="pepT"
                     /locus_tag="ACHKBL_001643"
     CDS             16828..18057
                     /gene="pepT"
                     /locus_tag="ACHKBL_001643"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HFO5818525.1"
                     /translation="MDKLLERFLNYVSLDTQSKAGVRQVPSTEGQWKLLHLLKEQLEE
                     MGLINVTLSEKGTLMATLPANVPGDIPAIGFISHVDTSPDCSGKNVNPQIVENYRGGD
                     IALGIGDEVLSPVMFPVLHQLLGQTLITTDGKTLLGADDKAGIAEIMTALAVLQQKNI
                     PHGDIRVAFTPDEEVGKGAKHFDVDAFDARWAYTVDGGGVGELEFENFNAASVNIKIV
                     GNNVHPGTAKGVMVNALSLAARIHAEVPADESPEMTEGYEGFYHLASMKGTVERADMH
                     YIIRDFDRKQFEARKRKMMEIAKKVGKGLHPDCYIELVIEDSYYNMREKVVEHPHILD
                     IAQQAMRDCDIEPELKPIRGGTDGAQLSFMGLPCPNLFTGGYNYHGKHEFVTLEGMEK
                     AVQVIVRIAELTAQRKS"
     gene            complement(18203..19324)
                     /gene="roxA"
                     /locus_tag="ACHKBL_001644"
                     /gene_synonym="ycfD"
     CDS             complement(18203..19324)
                     /gene="roxA"
                     /locus_tag="ACHKBL_001644"
                     /gene_synonym="ycfD"
                     /EC_number="1.14.11.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[50S ribosomal protein L16]-arginine
                     3-hydroxylase"
                     /protein_id="HFO5818526.1"
                     /translation="MEYQLTLNWPDFLERHWQKRPVVLKRGFNNFIDPISPDELAGLA
                     MESEVDSRLVSHQDGKWQVSHGPFESYDHLGETSWSLLVQAVNHWHEPTAALMRPFRE
                     LPDWRIDDLMISFSVPGGGVGPHLDQYDVFIIQGTGRRRWRVGEKLQMKQHCPHPDLL
                     QVDPFEAIIDEELEPGDILYIPPGFPHEGYALENAMNYSVGFRAPNTRELISGFADYV
                     LQRELGGNYYSDPDVPPRAHPADVLPQEMDKLREMMLELINQPEHFKQWFGEFISQSR
                     HELDIAPPEPPYQPDEIYDALKQGDVLVRLGGLRVLRIGDDVYANGEKIDSPHRPALD
                     ALASNIALTAENFGDALEDPSFLAMLAALVNSGYWFFEG"
     gene            complement(19400..20860)
                     /gene="phoQ"
                     /locus_tag="ACHKBL_001645"
     CDS             complement(19400..20860)
                     /gene="phoQ"
                     /locus_tag="ACHKBL_001645"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PhoQ"
                     /protein_id="HFO5818527.1"
                     /translation="MKKLLRLFFPLSLRVRFLLATAAVVLVLSLAYGMVALIGYSVSF
                     DKTTFRLLRGESNLFYTLAKWENNKLHVELPENIDKQSPTMTLIYDENGQLLWAQRDV
                     PWLMKMIQPDWLKSNGFHEIEADVNDTSLLLSGDHSIQQQLQEVREDDDDAEMTHSVA
                     VNVYPATSRMPKLTIVVVDTIPVELKSSYMVWSWFIYVLSANLLLVIPLLWVAAWWSL
                     RPIEALAKEVRELEEHNRELLNPATTRELTSLVRNLNRLLKSERERYDKYRTTLTDLT
                     HSLKTPLAVLQSTLRSLRSEKMSVSDAEPVMLEQISRISQQIGYYLHRASMRGGTLLS
                     RELHPVAPLLDNLTSALNKVYQRKGVNISLDISPEISFVGEQNDFVEVMGNVLDNACK
                     YCLEFVEISARQTDEHLYIVVEDDGPGIPLSKREVIFDRGQRVDTLRPGQGVGLAVAR
                     EITEQYEGKIVAGESMLGGARMEVIFGRQHSAPKDE"
     gene            complement(20860..21531)
                     /gene="phoP"
                     /locus_tag="ACHKBL_001646"
     CDS             complement(20860..21531)
                     /gene="phoP"
                     /locus_tag="ACHKBL_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PhoP"
                     /protein_id="HFO5818528.1"
                     /translation="MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHL
                     PDIAIVDLGLPDEDGLSLIRRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKP
                     FHIEEVMARMQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMET
                     LIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGY
                     LFELR"
     gene            complement(21700..23070)
                     /gene="purB"
                     /locus_tag="ACHKBL_001647"
     CDS             complement(21700..23070)
                     /gene="purB"
                     /locus_tag="ACHKBL_001647"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HFO5818529.1"
                     /translation="MELSSLTAVSPVDGRYGDKVSALRGIFSEYGLLKFRVQVEVRWL
                     QKLAAHAAIKEVPAFAADAIGYLDAIVASFSEEDAARIKTIERTTNHDVKAVEYFLKE
                     KVAEIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
                     YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQYRQLNQVEILGKINGAVGNYNAHI
                     AAYPEVDWHQFSEEFVTSLGIQWNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVW
                     GYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQ
                     RDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAIT
                     MVDELK"
     gene            complement(23074..23715)
                     /gene="hflD"
                     /locus_tag="ACHKBL_001648"
     CDS             complement(23074..23715)
                     /gene="hflD"
                     /locus_tag="ACHKBL_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HFO5818530.1"
                     /translation="MAKNYYDITLALAGICQSARLVQQLAHQGHCDADALHVSLNSII
                     DMNPSSTLAVFGGSEANLRVGLETLLGVLNASSRQGLNAELTRYTLSLMVLERKLSSA
                     KGALDTLGNRINGLQRQLEHFDLQSETLMSAMAAIYVDVISPLGPRIQVTGSPAVLQS
                     PQVQAKVRATLLAGIRAAVLWHQVGGGRLQLMFSRNRLTTQAKQILAHLTPEL"
     gene            complement(23751..24857)
                     /gene="mnmA"
                     /locus_tag="ACHKBL_001649"
                     /gene_synonym="trmU"
     CDS             complement(23751..24857)
                     /gene="mnmA"
                     /locus_tag="ACHKBL_001649"
                     /gene_synonym="trmU"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)(34)-
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HFO5818531.1"
                     /translation="MSETAKKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDD
                     GEEYCTAAADLADAQAVCDKLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILC
                     NKEIKFKAFLEFAAEDLGADYIATGHYVRRADVDGKSRLLRGLDSNKDQSYFLYTLSH
                     EQIAQSLFPVGELEKPQVRKIAEDLGLVTAKKKDSTGICFIGERKFREFLGRYLPAQP
                     GKIITVDGDEIGEHQGLMYHTLGQRKGLGIGGTKEGTEEPWYVVDKDVENNILIVAQG
                     HEHPRLMSVGLIAQQLHWVDREPFTGTMRCTVKTRYRQTDIPCTVKALDDDRIEVIFD
                     EPVAAVTPGQSAVFYDGEVCLGGGIIEQRLPLPV"
     gene            complement(24911..25372)
                     /gene="nudJ"
                     /locus_tag="ACHKBL_001650"
     CDS             complement(24911..25372)
                     /gene="nudJ"
                     /locus_tag="ACHKBL_001650"
                     /EC_number="3.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase NudJ"
                     /protein_id="HFO5818532.1"
                     /translation="MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEADETL
                     VEAAARELWEETGISAQPQHFIRMHQWIAPDKTPFLRFLFAIELEQICPTQPHDSDID
                     CCRWVSAEEILQASNLRSPLVAESIRCYQSGQRYPLEMIGDFNWPFTKGVI"
     gene            complement(25382..26035)
                     /gene="rluE"
                     /locus_tag="ACHKBL_001651"
     CDS             complement(25382..26035)
                     /gene="rluE"
                     /locus_tag="ACHKBL_001651"
                     /EC_number="5.4.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2457) synthase RluE"
                     /protein_id="HFO5818533.1"
                     /translation="MRQFIISENTMQKTSFRNHQVKRFSSQRSTRRKPENQPTRVILF
                     NKPYDVLPQFTDEAGRKTLKEFIPVQGVYAAGRLDRDSEGLLVLTNNGALQARLTQPG
                     KRTGKIYYVQVEGIPTEDALEALRNGVTLNDGPTLPAGAEMVEEPEWLWPRNPPIRER
                     KSIPTSWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYSLDNLANGEWREATD"
     gene            26207..27457
                     /gene="icd"
                     /locus_tag="ACHKBL_001652"
     CDS             26207..27457
                     /gene="icd"
                     /locus_tag="ACHKBL_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HFO5818534.1"
                     /translation="MESKVVVPAQGKKITLQNSKLNVPENPIIPYIEGDGIGVDVTPA
                     MLKVVDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKGP
                     LTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFRENSEDIY
                     AGIEWKADSADAEKVIKFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEYAI
                     ANDRDSVTLVHKGNIMKFTEGAFKDWGYQLAREEFGGELIDGGPWLKVKNPNTGKEIV
                     IKDVIADAFLQQILLRPAEYDVIACMNLNGDYISDALAAQVGGIGIAPGANIGDECAL
                     FEATHGTAPKYAGQDKVNPGSIILSAEMMLRHMGWTEAADLIVKGMEGAINAKTVTYD
                     FERLMEGAKLLKCSEFGDAIIKNM"
BASE COUNT         6705 a         7010 c         7128 g         6660 t
ORIGIN      
        1 atctatcatt tagttatcga tcgttaagta attgcttgcg acgtcattca tctgcataag
       61 gccactatta tgaaaaacgt aaaaaccctc atcgctgcgg cgattttaag ctcaatgtca
      121 tttgccagct ttgcggctgt cgaagttcag tcaacgccag aaggccaaca aaaagtcggt
      181 actatcagtg ctaacgcggg gacaaatctg ggatcgctgg aagagcagct ggcgcaaaaa
      241 gcggatgaga tgggcgcaaa atctttccgt attacttctg taaccggtcc gaataccctc
      301 catggaacag cagtaattta taaataagca ttaaccctca ttaatgcctg ctactgctga
      361 tttttttccc cgcgacatgc cgtgtcgcgg ggattttttt atccgagctt tacagagttt
      421 gtgggctcac ttgatgacgg cggacatcca ccggcatccc ggaacggaca tccatcacat
      481 gttgcatcac ttcagcgtca gtttgtgctg catctttaaa tgattgcatt gcgctattca
      541 gcgtaattgg cagcagctgc ggatcgtcat caatcttctc tgacagcggc tgatgtactt
      601 caaccagacg cgcaccgttt ggttcggcag agacttttat cggagtgttg atgatattca
      661 ctttggtgcc tggggtgacc tggctaaaga gtgttttgat atcgtcatcc cgcagacgaa
      721 tacagccaga acttacccgc atgccaatgc cgaaatcggc gttcgtaccg tggagcaaat
      781 aaacgccgcc ataggccgcc aggcgaatcg catgatggcc cattggatta tccggtccag
      841 ccggaactac cgcaggcaac tcaattccct gtgctttata acgggcgcgg atgtttgccg
      901 ttggcgtcca ggttgggttt gcacgtttgt ctgaaacggt ggtcaccatt gtcggcgtca
      961 gcgtgtcacc acctaactga ccaatgccga tgggatacac ggttaccgaa tttttacccg
     1021 gcgggtagta atagagacgc agttccgcaa tattgatcac aatgccttcg cgcggcgcat
     1081 ctggaagtag ggtttgcaac gggatcgtta acacgctgcc cgcgcgcggt acgtaaggat
     1141 caacgccggg gttagcctgt aacagagcga gaaagccgac gttatatttt ttggcgatgg
     1201 cttccagaga accaccgtca ttttccacca catgaaattt gttttcgcca accagacgac
     1261 tgcccgctgg cggcagcggc caggtgttgg cttttgccgg tagcgccagc gccacggcag
     1321 cggcgaacgt aaaaaacgtt agccagcgag aaaaacgcgt tttgatcatc accaaaaatc
     1381 cataataaat ataaggttat tgtaataaac agataagcct gaattatgga tggtgacagt
     1441 gtcggatagt gcagggaagt gcaaagaatt tgtaaatgtt gcagatgggg gcgcagaaac
     1501 gcccccgatt taccattaag cgatcgcgtt ctcttccagt tcacgcataa actggcgtac
     1561 ccattcgata cgcgttttcc gctcactcaa atcctgaata aacttcaggc gcgtcggacc
     1621 atcgaggcga taatgctgcg gctgtttttg cagcaaacca atcaaccagg ccggattaac
     1681 gtgattcttc tcggcaaatt cgatcacgcc gcctttctca ttaccttcca gcttcctgat
     1741 ccccagtttc tgcgcttgct ggcgcagacg ggcaatatcc agcagggtac gcgccggatc
     1801 cggcagcagg ccgaagcgat cgataagctc gactttgatc tcttccagtt cgttttccgt
     1861 tttggcgctg gcaatacgtt tgtagaacga cagacgcgtg ttcacgtcag ggatgaaatc
     1921 atcgggcaat agcgacggca tccgcagctc gacttctgtt tgctgactgg tgagatcttc
     1981 cagcgacggc tcgcgtccgg ctttcagcgc atcgacagcg ttttccagca actccatata
     2041 cagcgagaaa ccgatggttt ccattgagcc gctttgttct tcgccaagca gttcacctgc
     2101 gccgcggatc tccagatcgt gcgttgccag cgcaaaacct gccccgagat cttccagcga
     2161 ggcaatcgct tcaagacgtt tttgcgcatc ggtagtcatc gcttttggat gcggcgtcag
     2221 cagccatgca tatgcctggt ggtgcgaacg cccgacgcga ccgcgtaact ggtgcaactg
     2281 tgccagaccg aagtgatccg cgcgttcaat gataatggtg ttggcggtag gaatgtcgat
     2341 cccggtttcg ataatggtgg tacaaaccag cacgttgaaa cgttgatgat ggaaatcatt
     2401 catcacccgt tccagttcgc gctcacgcat ttgcccgtga ccgatggcaa tccgcgcttc
     2461 aggtaccagt tctgccagcc gttcggcggc tttctggatg ttttccacat cattgtagag
     2521 ataataaacc tgtcctccgc gcaaaatttc acgcaggatc gcctcccgga ccaccagact
     2581 gtcatactca cggacaaagg ttttaactgc cagacgacgg gcgggcggcg tggcgataat
     2641 cgacaggtca cgcattccgc tcattgccat attcagcgta cgtgggatcg gcgttgcagt
     2701 aagcgtcaga atatccacgt tcgcgcgcat cgctttaatg cgctctttat gacgcacccc
     2761 gaagcggtgt tcttcatcga caatcagcag gcctaaatct ttaaacttga cgtcactttg
     2821 cagcagtttg tgcgtaccga tcagaatatc gattttccct tccgctactt ccgcaaggat
     2881 ttgcgtctgc tctttggcgc tgcggaaacg ggagatcatt tcgatacgta ccggccagtt
     2941 ggcgaaacgg tcgcggaagt tgtcgtaatg ctgctgcgcg agaagggtgg ttggcaccag
     3001 caccgccacc tgcttgtggt tatctactgc caggaaagcg gcgcgcatcg ccacttctgt
     3061 tttaccaaag ccaacatcgc cgcacaccag acgatccatt gccagcggct gacacatgtc
     3121 gctaagtacc gcattaatgg cctgtgcctg atccggcgtg gtttcaaacg ggaagctgtc
     3181 gcagaacaac tgatactgct cgcgatcgtg tttaaacgcg aagccctctt tggcggcgcg
     3241 ttgcgcgtag atatccagca attctgctgc cacatcacgc actttttccg ccgctttctg
     3301 ccgcgcgcgt gaccaggcat cgccgccaag tttatgcagc ggggcgtttt cttccgcgcc
     3361 acccgcgtaa cggctaatca gatgcagtga cgacaccgga acatacagtt tggcgtcgtt
     3421 ggcataggtg agcatcaggt actcgccagt aataccgccc gcttccagcg tggtcattcc
     3481 ggcatagcgc ccgacaccgt gctccagatg gaccaccggc tgaccaatat gcagctccgc
     3541 gaggttacgg atcagtgtat cggggttgat ggcgcggcga gaatcctgac gacgacgggc
     3601 aacgcgttca ccgagcagat cgctttcgca aatcagcgcc agattacgta ccgtatcgac
     3661 aaaaccatgt tcggcagcgc caatcatcaa ataacgccca cggtcgctgg cttcatcaag
     3721 acgcataatg cgttgcggag caattttaat tcgcgcgagc agttcaccca gcgcttcacg
     3781 gcgaccttca ctttctaccg agaacaccac cggaccgtcg aaagactcga ggaacttacg
     3841 cagcgcatcc agcggtgctt tttgttgtgc ctgaacggcc aggtctggca gtttctggaa
     3901 acctaaattg gcattcgcgg cttttgtcgg taaatgttca gtttttagct gcacacgcgg
     3961 ccagtttttc agctctgaaa agagctcgtc tacccgcagc cagagcgatt gtggtggcaa
     4021 cagtgggcgc atcggatcga cgccgcgatt ttcaaaacgc gccagcgtgt cagcctggaa
     4081 acgttcggca ctgttttcca gatcgccagt attcaccaac agcgtattgg cagggaaata
     4141 actgaacagt ggcggcagcg gttcgctgaa gaatagcggc tgccagtact cgatcccggc
     4201 aggtaatgtg cctttactca cttgctgata aatatgttcc ggatcgcgct tcacttcgaa
     4261 ggtatcgcgc cactggctgc gaaacagttc aattgccgct ttatcggtcg gaaattcgtg
     4321 cgcgggcagc agattgatct cttctacttc ctccagcgtg cgctggctat cgacgtcaaa
     4381 cactcgcagg ctgtcgattt catcatcaaa gaaatcaaga cgataaggca gctcactgcc
     4441 catcgggaag agatccagca acgcgccgcg cgtggcgtat tcgccgtgct ccattacctg
     4501 gtcaacatgg cgataaccgg cgctgtccag ttgggttcgt aatgcatctc gtgacaggcg
     4561 ctgacctttt ttcatcacca gcgcatgacc gtggagaaaa ctgtgcgggc aaacgcgctg
     4621 cataagcgta ttcaccggaa caatcagtac gccacgctgc atggtcggta gctggtaaag
     4681 ggtggaaagg cgcgaggaga taatgtcctg atgaggcgaa aaactgtcgt agggaagggt
     4741 ttcccagtcc gccagattca tcaccatctg atcggtaaac tggctgattt catcatgcaa
     4801 acgcagagca ttttgcatat ctggtgcaat aagtaccacc ggaccggcgt gacgttcggc
     4861 aatttccgct accagcgtcg cgcaggcagc gccggttaac tcgcccagca gacgctgctc
     4921 acccgctttg acgggcagcg tataacgata ttgttcaggc ataacggttg tcagattctc
     4981 gttaggatat gcctcaacat atgggggcat atctctgata agcaatgctg ttcattatcc
     5041 gtaatcgaca tggtttagca aattgaatcg ccccggctgg ggcgatggct tagcgggagt
     5101 aactgacggc agcgggcagt gaaggcgggg agaacaacag atcgctgaat gcacgctgtg
     5161 aaagttttct taccagcaag cccgcaacag tacagatgaa caaactgaca aagggataaa
     5221 ccatcaggag tgtcagttcg acttgcggcg accagcgtgc cgcgttcatt ggcgcaagca
     5281 gagttaagct gaatatctct accagaatgc gatgggtggt gtagatagca atggtgttgg
     5341 tgccaatcac attcagcagg ctggtggagc gcataccgaa acgttgctcg tattgataaa
     5401 acagcttcat gatcaccaca atcgatacca gcgacaacag cagcgagata gtaaacaacc
     5461 aggcaccgac cgccagaacg gtcagcaaag aagtcatcag cagatggcgg cgcaacggca
     5521 cctctttaac gtaggtcatt aatgttgcgc caaaccatgc gccaaggctg taataaggca
     5581 aattgcggat cacactgttc attccccacc acggcgtggg aacgaaatta accgccacac
     5641 tcatcagtac aaacaaggcg aatagtggca gcgccagacg gctaaaaatt ttacatacca
     5701 cgaaataaac aattaacgca tacagatacc acaaactggt gctggcggtg atcatcccgt
     5761 gcaggaactc accggtggaa tcggcatacg cggcattgga ggcattgctt aaatcgcgct
     5821 caggtgccag ccactggttc agcgcactta acgccagcca ctgcaccacg ccccaaagcg
     5881 ccagcaccca gaagatgttc cagatccgtt tatcgagaca atttccccac ggtacgctgt
     5941 cgatataacg gcgaatcaaa tagccggata taaagaaaaa aaaccggcat acgaaagggt
     6001 gcaaggtaaa gattgaaata gatccagcat ttgctcagga cttccgataa cggatgctga
     6061 aaagtggtga gatgcggata aaaggtaatg accgagtgat aaatcaccac cagacaaata
     6121 cataaccctt tgatctggtt aatccatagc tctttttgtt tcatcaggac aacacgctct
     6181 ttttatttaa catgctgttg atcctgaggg gccagtcgcg tggtgtaatg cgtatctgtc
     6241 tgtatttggt tatttttcag ataaagatca ggctacaagg ccgatgagaa aatgaggccc
     6301 tttagccgat tgatttgctg gcacttttca gattagccct gacgatcact tacagttcag
     6361 acgtttaccc atcccgcttt cgcttatata ctcgtgtctt tgctaacagc aaccagacgg
     6421 atttcatgta ccaacctgtc gctctattta ttggcctgcg ttacatgcgt gggcgtgcag
     6481 cggatcgctt cggtcgtttc gtctcctggc tttctaccat cggcattacc ctcggggtga
     6541 tggcgctggt cacagtattg tcagtgatga acggctttga gcgcgagcta caaaacaaca
     6601 tccttggcct gatgccacag gcaattctct cttctgagca tggctctctt aacccgcagc
     6661 aactcccgga aacggcagtc aaactggacg gcgttaatcg cgtcgcacct attactaccg
     6721 gtgatgtggt actgcaaagc gcgcgcagcg tggcggtcgg ggtgatgctg ggtatcgacc
     6781 cggcacaaaa agatccacta acgccgtatc tggtcaatgt gaaacaaact gacctcgagc
     6841 cggggaaata taatgtcatc ctcggtgaac agctggcctc acagttgggc gtcaatcgcg
     6901 gtgatcaaat ccgcgtgatg gtgccatctg ccagccagtt cacgccgatg gggcgtattc
     6961 caagccagcg cctgttcaat gtgattggca ctttcgccgc caacagtgaa gtcgatggct
     7021 atgaaatgct ggtgaatatt gaggatgcct cgcgtctgat gcgttatccg gcaggcaata
     7081 ttaccggctg gcgtttgtgg ctggatgagc cgctgaaagt cgactcatta agtcaacaaa
     7141 aactgcctga aggcagcaaa tggcaggact ggcgtgaccg taaaggcgag ctgttccagg
     7201 ccgtacgcat ggaaaaaaat atgatgggct tgctgctgag cctgattgtt gccgtagcgg
     7261 cgtttaacat tattacctcg ctagggctaa tggtgatgga gaagcagggc gaagtcgcga
     7321 tcctgcaaac gcaaggctta actccgcgac aaatcatgat ggtctttatg gtgcaagggg
     7381 ccagcgccgg gattatcggt gcgatcctcg gagcggcgct tggcgcactg cttgccagcc
     7441 agttaaataa tctgatgccg ataatcggcg tcctgcttga tggcgcggcg ctgccggtcg
     7501 ctatcgaacc tttacaggtc attgttattg cgctggtggc gatggctatc gcgctgctgt
     7561 ctacgcttta cccttcatgg cgcgctgccg ccacacaacc cgctgaggct ttacgttatg
     7621 aataagatcc tgttgcaatg cgacaacctg tgcaaacgct atcaggaagg cagtgtgcaa
     7681 accgatgtgc tgcacaatgt cagtttcagc gtgggcgaag gtgaaatgat ggcaattgtc
     7741 ggtagctctg gttccggtaa aagtaccttg ctgcacctgt taggcgggct ggatacgcca
     7801 acctccggcg atgtgatctt taacggtcag ccgatgagca aactgtcgtc ggcggcgaaa
     7861 gcagaactgc gcaaccagaa gctgggcttt atttatcagt ttcaccacct gctgccggat
     7921 tttactgccc tggaaaacgt ggctatgccg ctgctgattg gcaagaaaaa gcccgctgaa
     7981 atcaacagcc gtgcgctgga gatgttaaaa gcggtggggc tggagcatcg tgcgaatcac
     8041 cgcccatctg aactttctgg cggcgaacgc cagcgcgtgg cgattgcccg tgcgctggtc
     8101 aataacccgc ggctggtgct ggcggatgaa cctactggta acctcgatgc acgtaacgcc
     8161 gacagcatct tccagttgct tggagaattg aatcgcttgc agggcaccgc cttcctggtg
     8221 gttactcacg acctgcaact ggcaaaacgt atgagccgcc aactggagat gcgtgatggt
     8281 cgtctgacgg cggaactgag cctgatgggg gcggagtaat ggcgatgcct ttatcgttat
     8341 tgattggcct gcgttttagc cgcggacggc ggcgcggcgg catggtgtcg ctgatctccg
     8401 tcatttctac cattggcatt gcccttggcg tggcggtatt gatcgtcggc ttaagcgcga
     8461 tgaacggctt tgaacgcgaa ctgaataacc gcattctggc ggtggtgccg catggcgaaa
     8521 tagaggcggt ggatcaaccg tggactaact ggcaggaagc cctggataac gtgcaaaaag
     8581 tgccaggtat tgccgccgct gcgccgtata tcaatttcac cggactggta gaaagtggtg
     8641 ccaatctgcg cgcaatccag gtgaagggcg ttaacccgca acaggaacag cgtctgagcg
     8701 cattaccctc gtttgttcag ggggatgcct ggcgcaattt taaagcgggc gaacagcaaa
     8761 ttatcatcgg caaaggcgtg gcggatgcgc tgaaagtgaa gcagggcgat tgggtgtcga
     8821 ttatgatccc caactcgaat cccgagcata aactgatgca gccaaaacgt gtgcgtttgc
     8881 acattgccgg tattttgcag ttgagtggtc aactcgatca cagttttgcc atgatcccgc
     8941 tggcggatgc ccagcaatat cttgatatgg gttccagcgt gtcaggtatt gcccttaaaa
     9001 tgacggatgt tttcaacgcc aataagctgg tacgcgatgc gggtgaagtg accaacagct
     9061 atgtttatat taaaagctgg attggtactt acggctatat gtatcgcgat atccagatga
     9121 tccgcgccat tatgtatctg gcgatggtac tggtgattgg cgtggcctgt ttcaacatcg
     9181 tctccacctt agtgatggcg gtgaaagaca agagtggcga tatcgcagta ttaagaacgc
     9241 tgggggcgaa agatggttta attcgcgcca tctttgtctg gtatggattg ctggcagggc
     9301 tgttcggtag cctgtgtggt gtgattatcg gcgtagttgt ttcactgcaa cttaccccga
     9361 ttattgagtg gattgaaaag ttgatcggtc atcagttcct ctccagcgat atctatttta
     9421 ttgacttcct gccatcggaa ttgcactggc tggacgtctt ctacgtactg gtcacagcat
     9481 tgttgctgag tcttttggca agttggtatc cggcgcgacg cgccagtaat attgaccctg
     9541 cgcgagtcct tagcggccag taaaggcagt acattaaaac aaggagcggc aatgtattac
     9601 gggtttgata ttggtggaac aaaaattgcg cttggcgtgt ttgatagcgg tcggcagttg
     9661 cagtgggaaa agcgggtgcc gacaccgcgt gacagctatg acgcattttt agatgcagtg
     9721 tgcgagctgg tagccgaagc tgaccggcgt tttggctgta aaggttctgt cggcatcggt
     9781 attccgggaa tgccggaaac agaagatggt acgctgtatg ccgccaatgt ccctgctgcc
     9841 agcggtaaac cgctgcgtgc cgacctgagc gcacgtcttg atcgcgatgt acgccttgat
     9901 aacgatgcca actgttttgc cctttctgaa gcatgggatg atgaatttac tcaatatcca
     9961 ctggtgatgg ggttgattct cggcaccggc gttggcggcg ggctgatttt caacggtaaa
    10021 cccattaccg gtaaaagcta tattaccggc gagtttggcc atatgcgtct gccggttgat
    10081 gcgttaacca tgatggggct ggatttcccg ttacgccgct gcggctgtgg tcagcatggc
    10141 tgcattgaaa attatctgtc tggtcgcggt tttgcgtggc tgtatcaaca ctattatcat
    10201 caaccgttgc aggctcccga aattattgcg ctttatgatc aaggtgatga gcaggcaagg
    10261 gcgcacgttg agcgttatct ggatttatta gcggtttgtc tgggaaatat cctgaccatt
    10321 gttgaccctg acctggtcgt cattggtggt ggcttatcga atttcccggc aatcacaacg
    10381 caactggcgg acaggctgcc tcgtcatctc ttacctgtag ctcgtgttcc gcgcattgaa
    10441 cgcgcgcgcc acggtgatgc gggaggaatg cgtggtgcgg ccttcctaca tctaaccgat
    10501 taaacaacag aggttgctat gctgtcgcgt cggggtcatc ggttaagtcg ttttcgtaaa
    10561 aataaacgcc gtctgcgcga gcgtttgcgt cagcgtattt ttttcagaga taaagtggtg
    10621 ccggaagcaa tggaaaaacc aagagtactc gtactgacag gggcagggat ttctgcggaa
    10681 tcaggtattc gtacctttcg cgccgcagat ggcctgtggg aagaacatcg ggttgaagat
    10741 gtggcaacgc cggaaggttt cgatcgcgat cctgaactgg tgcaaacgtt ttataacgcc
    10801 cgtcgtcgac agctgcagca gccagaaatt cagcctaacg ccgcgcatct tgcgctggcg
    10861 aaactgcaag acgccctcgg cgatcgcttt ttgctggtga cgcagaatat cgacaacctg
    10921 catgaacgcg caggtaatac caatgtcatt catatgcatg gggaactgct gaaagttcgc
    10981 tgttcgcaaa gtggtcaggt tctcgactgg accggagatg ttaccccaga agataaatgc
    11041 cattgttgcc agttcccggc acccttgcgc ccgcacgtag tgtggtttgg cgaaatgcca
    11101 ctcggcatgg atgaaattta tatggcgttg tcgatggccg atattttcat tgctattggt
    11161 acttcggggc atgtttatcc ggcggctggg tttgttcacg aagcgaaact gcatggcgcg
    11221 cacaccgtgg aactgaatct tgaaccgagt caggttggta atgaatttgc cgagaaatat
    11281 tacggtccgg caagccaggt ggtgccagag tttgttgaaa agttgctgaa gggattataa
    11341 agcgggaaaa attgcctgat gcgctacgct tgtcaggcct gtgaaggggc gcaattaatt
    11401 gaatttgcgg gttttgtggg ccggataagg cgttcgcgcc gcatccggca actttactgc
    11461 ctcttttacc gtttactcgt ccatagtaac gcggcaataa acgtacagac accaatcagc
    11521 cctgcccaga gcggcgatat tctggtaatc gcaaagctgt tatcgatata caaacataaa
    11581 gcctgatagc ccatattggc aacagccata gccactgcaa attttaacgc ataataaagg
    11641 cgcgttgtgt agttaaaaaa tgcagcaaag acgatgaatg caactgccca ggcaaaaagg
    11701 cctaatgtcg tggtactgcc gggaagatca ttctcctgct gttgtccggg atgcggataa
    11761 tatgtcagtg gcggcgtcgg ttgttttaat atctgtgctt gtaaaacagc caatgcatta
    11821 ttgattccct gataccagag atctgcatgt aaataagcca gagtgtgatt ttctaccaga
    11881 aggccgagat gtttatcgtc catgctgctt tcaacctggc cgttggttat cataattaac
    11941 cgtggctcgc gggtagtaaa aagtaacaga acacttcctt ttacattact cattgaagat
    12001 gccgcatgcg ccagcatcgc tttgccattt tgcctgaagc ttgcttcatt aacgccaggt
    12061 acaaccacga taaacggcgc aatcccggtt tgttgacgaa aaagttcggc tttacgcgcg
    12121 atctcttcgg catccggtac agtaccggta acatccgtaa aaagagtctt cattattgga
    12181 atcaatggct tactggttac cgtcagaggc aggagtatca gtaaactgag caatattatg
    12241 atttttttca tgggcgggac ttccgtttcc tgcgtgtctt tttagagtag ggcatactga
    12301 ttaataaacc gacggagata aaaataacgc ccataatgcc gataaacccc catttaaaaa
    12361 aattaaccca cgggttattt atctcagcct tttcaggatg tcctggagaa taataaacat
    12421 taactttatc gccttcactg tcttcataaa aatcgctgcc aatacttgaa ttgaaaatta
    12481 gcgtgtaatc tggcgtcgga cgaaaggcaa cgacgggata ccacgaacca tttttacccg
    12541 tgcgatgaga atgactggag tgccagacgg tatcaacaat gacgccttca gtatgaatcg
    12601 cgtctttgct ataggtgaac tggttataaa agataacaac agaaatgagg atcagtacca
    12661 gaccgatacc gaagaatata cgaaagaaaa ctctactgtc ctggctcatt agccacatcc
    12721 ttgctaaata aaaaacgggc ggtaatacca ccgcccgctt gctgaattaa cgtcctgctt
    12781 tcagcttctg ataatactct tcataaatgc tgctggctgc gccaacgtca ttctgccatt
    12841 caccattttt aatggtttca gcatccgggt agagcgtttt atcgttcgcc acttccggac
    12901 ttaacagctt acgcgccgca aggtttggcg ttggataacc gatagtttca gcaacctgtt
    12961 ttgccacatc cgggcgcagc aggaagttga tcaatttcag tgcgccttct ttgtttttgg
    13021 catttgccgg gatcgccagg ctgtccatcc agaaaatgcc gccttctttc ggccacacca
    13081 catcaattgg cgtacccgcc tggcgtgcaa cgaaagcaga accgttccag atcatgccga
    13141 ggttaacttc gccttccata tacgggttag cggggttatc ggagttaaac gccgccacgt
    13201 ttggcatcag ttttttcagc tcgttgtatg cagcttcaat ctctttcgga tcggtggtgt
    13261 taccggagta gcccagctta cgcagcgcca tctggaacac ttcacgggca tcgtcggtca
    13321 gcagcaggct gcctttgtac tcaggcttcc acagatcggc ccagctggtg acagatttcg
    13381 gatccaccgc atcaccgtta acgccaatcg ccgttgcacc ccagatatac ggaatggaat
    13441 agtcgttatt cgggtcaaac ggcttgttga gcatgtctgg atcgaggttg ctgaaattgc
    13501 ttaacttcga cttgtcgatc ttctggatca tcccttcttt acgcatttta tcgacgtaat
    13561 aggttgaagg aaccaccagg tcataggcac cgtctttgta cgttttcagc ttcgcgtaca
    13621 tggtttcgtt cgactcgtaa gtcgaataga taaccttaat accggtttct ttggtgaact
    13681 gttcaagcag tcctggcggc acgtactcgg tccagttgta gaaatacagc gtgttgttgt
    13741 catcggcgtg agcggcgctc atgcccagtg ccagagcacc cgccgcgagc aggtggcgtg
    13801 accatttttt catttaacgt cccctgtgtt acctttcgtt ttatcacgag caataagctg
    13861 gctggcaatt accatcacca gcgacagcac cagtaatatg gttgccagcg cgttaacttc
    13921 cggcgatacg ccgactttga ccatcgaata aatttttaac ggcagaattt cataactcgg
    13981 cccggtgaca aacgaagaaa ccaccacgtc gtccatcgac agggtaaagc ttaacaccca
    14041 gcccgccgct accgctggca ttgccagagg cagaatgatt tttcgcagaa tggtaaattc
    14101 gctggcaccg agatctttcg ccgcttccag cattcgcacg tcaaaacctt tcaggcgcga
    14161 atacactgtc accaccacaa atggcaggca gaaggtgata tgcgagaaca gcagcgacca
    14221 gaagccaagc tgaataccca gcagcataaa cagcaccagc agcgaaatcg ccatcacgat
    14281 atctggtgac atcatcacca caaacagcat tccgctaacg aacggcttac cacgaaagcg
    14341 ataacggtac agcgcaactg ctgtcagtga accgataagc gtagcaaacg tcgccgaaaa
    14401 caccgccatt gttagtgaat gctgcgctgc ctgtaacagg ctgtcgttgt tcatcagcag
    14461 gctataccat ttggtggtaa aaccctgcca gttgatgcca aagcgcgagc tgttaaagga
    14521 gttcacaatc aaaataatga ttgggatata caggtacgcg tagatagcgg tcataaaacc
    14581 gccgcgaagc agtcgaccga tcattcgagt tccaccttct tattcagcag acgagaagcg
    14641 cgccagtaaa ccagcaacat caggcccatt acgatagtca gcgtaatgct ggtggctgca
    14701 ccaaacggcc agtcacgaat attgaggaac tgtaccttga tgacgttacc gatcagcagg
    14761 tttttcgcac cgcccatcag gtcggacaca tagaacaggc ccatcgctgg cagcatcacc
    14821 agcagacatc cggcaataat tcccggcatc gttagtggga taatgatgcg gataaaggtc
    14881 tgcaacttgc tggcaccgag atcgcgcgcc gcctcaagca acggcttatc cagtttttcg
    14941 atactggagt acagcggcat caccataaac ggcagcagaa tgtaaaccag accgataatc
    15001 accgcactag gcgtgaacat gatgcgaatt ggtgtatcga taacgcccag ccagagcaaa
    15061 aactcgttga gatagccttt ggtgctgagg aaaattttca gcccgtaaat acgaatcaat
    15121 gagttggtcc agaacggaac aatcagcaga aacagcagca gcggacgcac cttgtgtggc
    15181 agcttcgcca gaaaccaggc aaaaggatag cccagcacca ggcaggcgag ggtggcgatc
    15241 agcgccatat tcagcgagtg caatagcact tcaaaataga gcggatcgag cagacgcgtg
    15301 tagttatcca gcgtaaagac cattttgacg aaactggcgt cgtcgcgggt caaaaagctg
    15361 gtgccaatga tcatcaggtt gggcagaaag acaaacaaca caagccaacc gacaatagtg
    15421 acaatcacta cattctggaa cttacttgtg ttcttcatca gccagtacga cctcccagct
    15481 ttctacccaa ttaatggcca ttttttggtc gagagagtgg tcaaagtcag gatcgtcttc
    15541 attgaagaat tcgctgacca tcaccatctt gccattttcc agctcaacaa ccgactccag
    15601 cgtcatgcct ttgtagttac gctcgcgaac gtaaccaatc agcccttcgg cgtggttgtc
    15661 gtcgttaatc tcttcaacac gtaagtcttc cgggcgcagc agaacatgca gtttttgccc
    15721 cggttcaacg gtgaagttaa cgtagatatt acattcgcgg ccttcaacgt tggcgcgtac
    15781 gcgctgctcg tcgagacgtt cgatgacagt ggcgttaaac atattgattt cgccaataaa
    15841 cccggcaaca aacaggtttt tcggctcttc gtagatttca cgcggcgtgc cgtcttgctc
    15901 aatgcgacca tcgcgcatca ccacgatcct gtccgacatg gtaagtgctt cttcctggtc
    15961 gtgagttacg aacacgaatg taataccaag cttacgctgt aacgctttca gctcgttctg
    16021 catttgcttg cgcagtttgt aatccagcgc tgagagcgac tcatccagca acaacagacg
    16081 aggcttgtta accaccgcgc gggcaatggc gacgcgttgc tgttgaccac cagagagctg
    16141 atgcggtttg cgttgagcga aggtttccag ctgcaccatc cgcagggctt ccatcacgcg
    16201 gggcgtaatt tcatcagcgg gggttttttg catgcgcaac ccaaaggcca cattttcgaa
    16261 cacggtcatg tgggggaaaa gtgcgtagct ttggaaaaca gtgttcacgt agcggttttc
    16321 cgccggaacg tgggtgatgt cctcgttatc caacatgatg cgtccggaat caacagtttc
    16381 cagacctgca atcaggcgaa gaacggttgt tttaccgcag ccagaagggc caagcagcgt
    16441 gaggaactcg ccattgttga tagtcagatc cagctgggga atgacctctt taccatcaaa
    16501 gcatttgcga attcccgcca attgcaccag cggtgaaagc gaactcggtt gtttattcaa
    16561 ttttttactc tgtcccatgt aaacgcaacg gatggcttac cgatgcgggg tttgtggtta
    16621 accaccttgg tgactcttaa tgagggcggt aattctacgg caaaccgctt gaatcgccaa
    16681 tctttgttgt gaattactgg cttagcttta tattcattaa ggtaatgctg ataaatattc
    16741 ccgcttgcag ggctaaaagt gacctgacgc aatatttgtc ttttcttgct tcttaataat
    16801 gttgtcacaa aaagtgaggg tgactacatg gataaactac ttgagcgatt tttgaactac
    16861 gtgtctctgg atacccaatc aaaggcaggg gtgagacagg ttcccagcac ggaaggccaa
    16921 tggaagttat tgcatctgct gaaagagcag ctcgaagaga tggggcttat caatgtgacc
    16981 ttaagtgaga agggcacttt gatggcgacg ttaccggcta acgtccctgg cgatatcccg
    17041 gcgattggct ttatttctca tgtggatacc tcaccggatt gcagcggcaa aaatgtgaat
    17101 ccgcaaattg ttgaaaacta tcgcggtggc gatattgcgc tgggtatcgg cgatgaagtt
    17161 ttatcaccgg ttatgttccc ggtgctgcat cagctactgg gtcagacgct gattaccacc
    17221 gatggtaaaa ccttgttagg tgccgatgac aaagcaggta ttgcagaaat catgaccgcg
    17281 ctggcggtat tgcaacaaaa aaacattccg catggtgata ttcgcgtcgc ctttaccccg
    17341 gatgaagaag tgggcaaagg ggcgaagcat tttgatgttg acgccttcga tgcccgctgg
    17401 gcttacaccg ttgatggtgg tggcgtaggc gaactggagt ttgaaaactt caacgccgcg
    17461 tcggtcaata tcaaaattgt cggtaacaat gttcacccgg gcacggcgaa aggagtaatg
    17521 gtaaatgcgc tgtcgctggc ggcacgtatt catgcggaag ttccggcgga tgaaagcccg
    17581 gaaatgacag aaggctatga aggtttctat cacctggcga gcatgaaagg caccgttgaa
    17641 cgggccgata tgcactacat catccgtgat ttcgaccgta aacagtttga agcgcgtaaa
    17701 cgtaaaatga tggagatcgc caaaaaagtg ggcaaagggt tacatcctga ttgctacatt
    17761 gaactggtga ttgaagacag ttactacaat atgcgcgaga aagtggttga gcatccgcat
    17821 attctcgata tcgcccagca ggcgatgcgt gactgcgata ttgaaccgga actgaaaccg
    17881 atccgcggcg gtaccgacgg cgcgcagttg tcgtttatgg gattaccgtg cccgaacctg
    17941 ttcactggcg gttacaacta tcatggtaag catgagtttg tgactctgga aggtatggaa
    18001 aaagcggtgc aggtgatcgt ccgtattgcc gagttgacgg cgcaacggaa gtcgtgattg
    18061 cccgatgcgt atgtggactg ccaattaccg ggatgaaacc tttatattga cactggtcca
    18121 catacttatc gtgcctacag gcgttcagtg catgtaggca cgataaggcg caatcagcgc
    18181 cgcatccggc ttaaatccaa acttaccctt cgaagaacca gtatccgcta ttgaccagcg
    18241 ccgcgagcat cgcgaggaat gacggatctt ccagcgcatc gccaaaattc tccgcggtca
    18301 gcgcaatgtt gctggcgagc gcatccagtg ccggacggtg cggggaatcg atcttctcac
    18361 cattggcata cacgtcgtcg ccaatgcgca atacgcgcag accccccagg cgcaccagca
    18421 catcaccttg tttcagcgca tcgtagattt catccggctg ataaggcggc tctggcggcg
    18481 cgatatccag ttcatgacgt gactgggata taaactcgcc aaaccattgc ttaaaatgtt
    18541 ccggctggtt gatcaattcg agcatcatct cacgcagttt atccatctct tgcggcagaa
    18601 cgtccgcagg atgagcgcga ggtggaacat ccggatcgct gtagtagttg ccgcccagtt
    18661 cacgttgcag cacataatcg gcaaatccac taatcagttc ccgcgtattt ggcgcgcgaa
    18721 aacccacgga atagttcatc gcattttcca gcgcgtagcc ttcatgcggg aatcctggcg
    18781 gaatataaag aatatcacca ggctccagct cttcatcgat gatggcttcg aacggatcga
    18841 cctgtaacag atccggatgt gggcagtgct gtttcatttg cagcttttcg cccactcgcc
    18901 agcgacgacg tccggtaccc tgaatgataa acacgtcgta ctgatcgaga tgcgggccga
    18961 cgccgccgcc aggtacagaa aaagagatca tcaggtcatc aatacgccag tccggcagtt
    19021 cacggaacgg tcgcatcagc gcggcggtcg gctcatgcca gtggttcact gcttgcacta
    19081 acaatgacca gctagtttca ccgagatgat cgtagctttc gaatggaccg tggctgacct
    19141 gccatttgcc atcctgatga ctgaccagtc gactgtcgac ttcgctttcc atcgccagac
    19201 ccgccaactc gtctggagag atcgggtcaa taaaattatt aaagccgcgt tttaacacca
    19261 ccgggcgttt ctgccagtga cgttcaagaa aatcgggcca gttaagagtg agttggtatt
    19321 ccatgttaag catccacagg ctggtatctg caaccgatta taacggatgc ttaacgtaat
    19381 gcgtgaagta tggacatatt tattcatctt tcggcgcaga atgctggcga ccaaaaatca
    19441 cctccatccg cgcaccgccc agcatgctct ctccggcgac gattttaccc tcatattgct
    19501 cggtgatttc gcgggctacc gccagcccca caccttgccc agggcgtaaa gtatcaaccc
    19561 gttgaccacg gtcgaaaatg acctctcgct tgcttaatgg aataccgggg ccatcatcct
    19621 cgaccacaat atagagatgc tcgtcggttt gccttgcaga aatttcgaca aactcgaggc
    19681 aatatttaca ggcattatcc agcacattgc ccatcacctc gacaaaatcg ttctgctcac
    19741 cgacaaagct gatctctggc gaaatatcga gagagatatt gacccctttg cgttgataca
    19801 ctttgttcag cgccgaggtg agattgtcca gcagtggagc gaccggatgc agctcgcggc
    19861 tgagcaatgt cccgccgcgc atactggcac gatgcaggta gtagccaatt tgctgtgaaa
    19921 tgcggctgat ttgctccagc attaccggtt cagcatcact gacgctcatc ttttcactac
    19981 gcagagaacg cagcgtactt tgcagcaccg ccagtggcgt tttcagacta tgggtcaggt
    20041 cggtgagcgt cgtacgatat ttgtcgtaac gttcgcgttc actttttaac aatcggttca
    20101 ggtttcgtac cagactggtc agttctcgcg ttgtggctgg attgagcaat tcgcggttat
    20161 gttcttccag ttcgcggact tcttttgcca gggcttcgat ggggcgtaaa ctccaccagg
    20221 cggcgaccca cagcagcggg atcactaaca gcagattggc tgagagcaca tagataaacc
    20281 agctccagac catataggaa ctttttagct ccaccggaat ggtatccacc accacaatgg
    20341 tcaactttgg catccgcgat gttgccgggt agacgtttac cgccaccgag tgggtcatct
    20401 ccgcgtcgtc atcatcttcc cgcacttcct gcaactgttg ctgtatcgaa tgatctccac
    20461 tcagcaagag gctggtatcg ttaacatccg cttcaatttc atgaaaacca ttcgatttca
    20521 gccagtcagg ctggatcatc ttcatcagcc agggcacgtc acgttgcgcc cataaaagct
    20581 gcccgttctc atcataaatt agtgtcatcg tggggctttg cttgtcgata ttttcgggta
    20641 actcgacatg caacttattg ttttcccact tcgcaagggt atagaacaga ttgctctcgc
    20701 cacgtaacag ccgaaacgtg gttttatcga aactgacgct ataaccgatc agcgcgacca
    20761 ttccgtaggc aagcgaaagc accagtacta ccgctgccgt tgccaacaga aaacgtaccc
    20821 gcagcgagag cgggaaaaaa agacgcagta attttttcat cagcgcaatt cgaacagata
    20881 gccctggccg cgaacggtgg taatcacttc ttgcggatat tgtgcctgaa tttttttgcg
    20941 cagacgtccc atcagtacat caatggtatg gctttcccgc agctccgcat ccggatagag
    21001 ttggagcatt aacgaatctt tgctcaccac tttgccatta ttgcgtatca acgtttccat
    21061 aatggtgtat tcgaacgcgg tcagtttgat cacttcgtca ttaatagata attcacggcg
    21121 agagagatca acctgaaacg gtggtagcga aatgacctgt gaagccagac cgctattacg
    21181 ccgcattaat gcctgcattc gcgccatcac ctcttcaata tgaaacggtt tagtcacgta
    21241 atcatcagcg ccagcactta atacttcgac tttgtcctgc cagctttcac gggcggttaa
    21301 taccagaatc ggcagtgaaa catcattgct acgccagcgg cgaatcagtg acagaccgtc
    21361 ctcgtcaggc aaaccgagat cgacaatcgc aatatccggt aaatgttcat tgagataata
    21421 atcggcttct ttggcatctt ctgcgtcatc gacctgatga ccagcatcct gaatctgaac
    21481 tttaagatgg tgacgtaaca acgcattgtc ttcaacaacc agtacgcgca tttttatttc
    21541 tccctgtctt aattattaaa atagtgtaac gcgattatgt ggttatgggg gtaaacatta
    21601 aataaaccag cgggaagggg aggtaaagtg aaaaaacaaa aagtggataa tcttaataag
    21661 caggccggac agcatcgcca tccggcactg atttgaggct tatttcagct catcaaccat
    21721 ggtgatggcg cgaccaatat agttagccgg cgtcatcgct ttcaggcggg ctttctcttc
    21781 ttctggcaac gccagaccgt cgataaactg cttcatgcct tcggcgtcaa cgcgcttacc
    21841 gcgagtcagc tctttcagct tctcgtacgg tttttcgatg ccatagcgac gcataactgt
    21901 ctggattggc tcagccagca cttcccagtt gtgatccagt tcatccagca gatggtcacg
    21961 gttcacttcc agtttgctca cccctttcag ggtggattga tacgcaatca gcgcataacc
    22021 gatacccacg ccgaggttac gcagcacggt ggagtcggtc aggtcacgct gccagcggga
    22081 aaccggcagt ttgcttgcca gatgctgcaa taccgcgttg gaaaggccca gattcccttc
    22141 ggagttttcg aagtcgatcg ggttaacttt atgcggcatg gtggaagaac caatctcacc
    22201 agcaatggtt ttctgtttga agtggttaag ggcgatataa ccccagacgt cacggtcaaa
    22261 gtcgatcaga atggtgttga agcgcgcaac gcaatcaaac agctcggcaa tgtagtcgtg
    22321 cggttcgatc tgggtagtgt acgggttcca ctgaataccc agcgaggtga cgaactcttc
    22381 gctgaactga tgccagtcaa cttccgggta agcggcgatg tgggcgttat agttaccgac
    22441 tgcgccgttg attttaccga ggatctccac ctgattaagc tggcggtact ggcgctccat
    22501 acggtaggcg acgttagcca tctctttacc gatggttgac ggcgtggctg gctgaccgtg
    22561 ggtacgggac agcagcggga tatcgcgata ctgaacggcg agatctttaa tgccatcaat
    22621 cagttgacgc cagtacggca ggatcacttc atcacgcgcg gttttcagca tcaacgcgtg
    22681 ggagaggtta ttgatatctt ccgaagtaca ggcaaagtgg atgaattcag aaaccgcatg
    22741 cagttccggg atctccgcca ctttttcttt caggaaatac tcaaccgctt taacgtcgtg
    22801 gttagtggta cgctcgatgg ttttgatgcg tgctgcatct tcttcgctga aactggcgac
    22861 aattgcatca aggtaaccga ttgcgtcggc agcaaaagca ggaacttcct tgatcgctgc
    22921 gtgcgcggcc agtttttgca gccaacgtac ttcaacttgt acacggaatt tcagcaaacc
    22981 atattcgctg aaaatcccgc gcagcgcgct gactttatcg ccgtagcgtc catcgacagg
    23041 ggaaacggcg gtcagtgagg ataattccat agatcacaac tccggggtta aatgagcaag
    23101 aatttgtttt gcctgagtgg tcaggcgatt acgagaaaac atcagttgca gacgtccgcc
    23161 gccgacctgg tgccagagca cggcggcgcg aatgcctgcc agcagggtag cgcgaacttt
    23221 cgcctgcact tgtgggcttt gcagtacagc aggggaaccg gtgacctgaa tgcgcgggcc
    23281 aagcgggcta atcacatcaa catagatagc agccatcgcg ctcatcagcg tttcggactg
    23341 taaatcgaag tgttcgagct ggcgttgcag gccgttgatc cggttgccca gagtgtcgag
    23401 cgcacctttc gctgaggaga gtttgcgctc aagcaccatc aggctgagtg tgtagcgggt
    23461 taactcagcg tttaagccct ggcgactgct ggcattgagc acgccgagca aggtttccag
    23521 cccgacgcgc aggttggctt cgctaccgcc aaaaaccgcc agcgtcgagc tggggttcat
    23581 atcaataata ctgttgagtg agacgtgtag cgcatcggca tcacaatgcc cctggtgagc
    23641 gagttgttgc accaggcgtg ccgactgaca aataccggcc agagcgaggg tgatgtcata
    23701 gtaattcttt gccacgttca ctgcttcctt gttttaagta aagaaaataa tcagaccggc
    23761 agcggcagac gctgctcaat aataccgcca ccgaggcaca cttcaccgtc atagaatacg
    23821 gcagactggc ccggcgtcac ggcggcaacc ggctcatcga aaatcacttc aatgcgatca
    23881 tcgtccagcg ccttgacggt gcacggaatg tcggtctggc gatagcgggt ttttaccgtg
    23941 caacgcatag tgccggtgaa tggttcgcga tcgacccagt gcaactgctg ggcaatcaga
    24001 ccgacagaca tcagacgcgg gtgttcatgg ccctgcgcaa caatcagaat gttgttttcg
    24061 acgtctttat cgaccacata ccacggttct tcggtgcctt ctttggtgcc accaataccc
    24121 agacctttac gctgaccgag ggtgtgatac atcagcccct ggtgttcacc aatttcatcg
    24181 ccatcgacgg taatgatttt gcccggttgc gccgggagat aacggcccag gaactcgcgg
    24241 aatttacgtt cgccaatgaa gcaaatgccg gtagagtctt ttttcttcgc ggtgaccaga
    24301 ccaagatctt cagcaatctt acgcacctgc ggtttttcca gttcgccgac cgggaacagg
    24361 ctttgcgcaa tctgctcatg gctgagcgta taaaggaagt agctctggtc tttattgctg
    24421 tccagaccac gcagcaggcg gctcttgcca tcgacatctg cccgacgtac gtaatgaccg
    24481 gtagcgatat aatcggcacc taaatcttcg gcggcaaatt cgaggaaggc tttaaatttg
    24541 atctctttgt tgcacagaat atccggattc ggcgtgcgac cggctttata ttcggcaagg
    24601 aacagttcga agacgttgtc ccagtactcg gcagcaaagt taacggtgtg cagttcaatg
    24661 ccgagcttgt cgcagacagc ctgggcatca gccagatccg ctgccgctgt gcaatattcc
    24721 tcaccgtcgt cttcttccca gttcttcata aacaggcctt cgacctgata tccctgttgt
    24781 tgcaacagcc aggcagaaac ggaggaatcg acaccgccgg acatgccgac gattactttt
    24841 tttgcggttt cagacattgg atcactcacg acattgaact tcaaggcggc gtattctatc
    24901 acgcagcctt ttagatgaca ccctttgtaa aaggccagtt aaaatcgcca atcatctcca
    24961 gcggatagcg ttgcccgctt tgataacaac gaatactttc cgccaccagc ggcgaacgca
    25021 gatttgacgc ctgtaaaatt tcttcggcgc tgacccaacg gcagcagtcg atatcgctgt
    25081 catgaggctg ggtcgggcat atttgctcaa gctcaatagc aaacaggaaa cgcaaaaacg
    25141 gcgttttatc tggcgcaatc cactgatgca tacgaataaa gtgttgcggc tgcgcgctga
    25201 tgccggtttc ttcccacagc tcacgcgcag cagcttccac tagcgtctca tccgcttcca
    25261 gatgcccggc aggttggttc cataacgctt taccattaat cgtctcttca acgactaaaa
    25321 atttgccttc tgcgtgcacc acgcaggcaa cggtaacgtg cggtttaaac attttgcgtc
    25381 cttaatctgt cgcttctcgc cattcgccat tggcaagatt atccaaagag taatcaccca
    25441 tcgcatagcg aatcagtcgc agcgtgggga aaccaacatg ggcggtcatg cggcgcacct
    25501 ggcgatttcg tccttcatat aaggtgatct tcagccagct ggtgggaata cttttgcgtt
    25561 cacgaattgg cggattacgg ggccataacc attccggctc ctcaaccatc tctgcacctg
    25621 cgggcagggt agggccatca tttaaggtta cgccattgcg caacgcttca agggcgtctt
    25681 ccgtgggaat accttctacc tgtacataat agattttccc ggtacgttta cccggctggg
    25741 ttaaacgcgc ctgcaacgcg ccgttattgg tcagcaccag taacccttcg ctatcgcggt
    25801 caaggcgacc tgctgcataa acgccttgaa ccgggatgaa ttcttttaat gttttgcgtc
    25861 cggcttcatc ggtgaactgc ggaagaacat cgtagggttt attgaacagg atcacacgcg
    25921 tgggctggtt ttcaggttta cgcctggtag aacgttgcga gctgaatcgc ttaacctggt
    25981 gatttctaaa agaagttttt tgcatggtat tttcagagat tatgaattgc cgcattatag
    26041 cctaataacg cgcatctttc atgacggcaa acaatagggt agtattgaca agccaattac
    26101 aaatcattaa caaaaaattg ctctaaagca tccgtatcgc aggacgcaaa cgcatatgca
    26161 acgtggtggc agacgagcaa accagtagcg ctcgaaggag aggtgaatgg aaagtaaagt
    26221 agttgttccg gcacaaggca agaagatcac cctgcaaaac agcaaactca acgttcctga
    26281 aaatccgatt atcccttaca ttgaaggtga tggaatcggt gtagatgtaa ccccagccat
    26341 gctgaaagtg gtcgacgctg cagtcgagaa agcctataaa ggcgagcgta aaatctcctg
    26401 gatggaaatt tacaccggtg aaaaatccac acaggtttat ggtcaggatg tctggctgcc
    26461 tgctgaaacc cttgatctga ttcgtgaata tcgcgttgcc attaaaggcc cgctgaccac
    26521 tccggttggt ggcggtattc gctctctgaa cgttgccctg cgccaggaac tggatctcta
    26581 catctgcctg cgtccggtac gttactacca gggcacccca agcccggtta aacaccctga
    26641 actgaccgat atggttatct tccgtgaaaa ctcggaagac atttatgcgg gtatcgaatg
    26701 gaaagcagac tctgccgacg ccgagaaagt gattaaattc ctgcgtgaag agatgggcgt
    26761 gaagaaaatt cgtttcccgg aacattgcgg tatcggtatt aagccgtgtt ctgaagaagg
    26821 caccaaacgt ctggtgcgtg cagcgatcga atacgcaatt gcgaacgatc gtgactctgt
    26881 gaccctggtg cacaaaggca acatcatgaa gttcaccgaa ggcgcgttta aagattgggg
    26941 ctaccagctg gcgcgtgaag agtttggcgg tgaactgatc gacggcggcc cgtggctgaa
    27001 agttaaaaac ccgaataccg gcaaagagat cgtcattaaa gatgtgattg ctgatgcatt
    27061 cctgcaacag atcctgctgc gtccggctga atatgatgtt atcgcctgta tgaacctgaa
    27121 cggtgactac atttctgacg ctctggctgc acaggtcggc ggtatcggta tcgccccagg
    27181 cgcaaacatc ggtgacgaat gcgccctgtt tgaagccacc cacggtactg cgccgaaata
    27241 tgccggtcag gacaaagtta accctggctc tattattctc tccgctgaga tgatgttacg
    27301 ccatatgggc tggactgaag ccgcagacct gattgttaaa ggtatggaag gcgcaatcaa
    27361 cgccaagact gtaacctatg acttcgaacg tctgatggaa ggcgctaaac tgctgaaatg
    27421 cagcgagttt ggtgatgcga tcatcaagaa catgtaatct ctacatgtgt taaatattga
    27481 aacgggcgta taacacgccc gtt
//
LOCUS       DAZFLD010000047        27410 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.047, whole genome shotgun sequence.
ACCESSION   DAZFLD010000047 DAZFLD010000000
VERSION     DAZFLD010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27410
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.047"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..415
                     /gene="cmtB"
                     /locus_tag="ACHKBL_001653"
     CDS             <1..415
                     /gene="cmtB"
                     /locus_tag="ACHKBL_001653"
                     /EC_number="2.7.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IIA"
                     /protein_id="HFO5818535.1"
                     /translation="SISVIHSAKDWQEAIDFSMASLLDKNYISENYIQAIKDSTINNG
                     PYYILAPGVAMPHARPECGALKTGMSLTLLKQGVYFPGNDEPIKLLIGLSAADADSHI
                     GAIQALSELLCEEEILNQLLTAASEKQLADIISRG"
     gene            443..1831
                     /gene="cmtA"
                     /locus_tag="ACHKBL_001654"
     CDS             443..1831
                     /gene="cmtA"
                     /locus_tag="ACHKBL_001654"
                     /EC_number="2.7.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HFO5818536.1"
                     /translation="MENKSARAKVQAFGGFLTAMVIPNIGAFIAWGFITALFIPTGWL
                     PNEHFAKIVGPMITYLLPVMIGSTGGHLVGGKRGAVMGGIGTIGVIVGAEIPMFLGSM
                     IMGPLGGLVIKYIDKALEKRIPAGFEMVINNFSLGIAGMLLCLLGFEVIGPAVLIANT
                     FVKECIEALVHAGYLPLLSVINEPAKVLFLNNAIDQGVYYPLGMQQASVNGKSIFFMV
                     ASNPGPGLGLLLAFTLFGKGMSKRSAPGAMIIHFLGGIHELYFPYVLMKPLTIIAMIA
                     GGMSGTWMFNLLDGGLVAGPSPGSIFAYLALTPKGSFLATIAGVTVGTLVSFAITSVI
                     LKMEKTVETESEDEFAQSANAVKAMKQEGAFSLSRVKRIAFVCDAGMGSSAMGATTFR
                     KRLEKAGLAIEVKHYAIENVPADADIVVTHASLEGRVKRVTDKPLILINNYIGDPKLD
                     TLFNQLTAEHKN"
     gene            1846..3123
                     /gene="yggP"
                     /locus_tag="ACHKBL_001655"
     CDS             1846..3123
                     /gene="yggP"
                     /locus_tag="ACHKBL_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HFO5818537.1"
                     /translation="MKTKVAAIYGKRDVRLREFELPEITDNELLVSVISDSVCLSTWK
                     AALLGSEHKRVPDDLENHPVITGHECAGVIVEVGKNLTDKYKKGQRFVLQPAMGLPSG
                     YSAGYSYEYFGGNATYMIIPEIAINLGCVLPYHGSYFAAASLAEPMCCIIGAYHANYH
                     TTQYVYEHRMGVKPGGNIALLACAGPMGIGAIDYAINGGIQPSRVVVVDIDDKRLAQV
                     QKLLPVELAASKGIELVYMNTKGMSDPVQTLRALTDDAGFDDVFVYAAVPSVVEMADE
                     LLAEDGCLNFFAGPTDKNFKVPFNFYNVHYNSTHVVGTSGGSTDDMKEAIALSATGQL
                     QPSFMVTHIGGLDAVPDTVLNLPDIPGGKKLIYNGVTMPLTAIADFAEKGKTDPLFKE
                     LARLVEETHGIWNEQAEKYLLAQFGVDIGEAAQ"
     gene            3120..3435
                     /locus_tag="ACHKBL_001656"
                     /pseudo
     CDS             3120..3435
                     /locus_tag="ACHKBL_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417405.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class II"
     gene            3457..3966
                     /gene="fumE"
                     /locus_tag="ACHKBL_001657"
     CDS             3457..3966
                     /gene="fumE"
                     /locus_tag="ACHKBL_001657"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarase E"
                     /protein_id="HFO5818538.1"
                     /translation="MATLTEDDVLEQLDAQDNLFSFMKTAHSILLQGIRQFLPSLFVD
                     NDEEIVEYAVKPLLAQSGPLDDIDVALRLIYALGKMDKWLYADITHFSQFWHYLNEQD
                     ETPGFADDMTWDFISNVNSITCNATLYDALKAMKFADFAVWSEARFSRMVKTALTLAV
                     TTTLKELTP"
     gene            3963..4676
                     /gene="yggC"
                     /locus_tag="ACHKBL_001658"
     CDS             3963..4676
                     /gene="yggC"
                     /locus_tag="ACHKBL_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HFO5818539.1"
                     /translation="MKIELTVNGLKIQAQYQNEEIENVHKPLLHMLAALQTVNPQRRT
                     VVFLCAPPGTGKSTLTTFWEYLAQQDPELPAIQTLPMDGFHHYNSWLDAHQLRPFKGA
                     PETFNVAKLAENLCRVVEGDCTWPQYDRQKHDPVEDALHVTAPLVIVEGNWLLLDDEK
                     WQELASFCDFSIFIHAPAQILRERLISRKIAGGLSRQEAEAFYARTDGPNVERVLVNS
                     RQANLILEMTEEGRYHFKS"
     gene            4961..5980
                     /gene="epd"
                     /locus_tag="ACHKBL_001659"
     CDS             4961..5980
                     /gene="epd"
                     /locus_tag="ACHKBL_001659"
                     /EC_number="1.2.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0048001 - erythrose-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HFO5818540.1"
                     /translation="MTVRVAINGFGRIGRNVVRALYESGRRAEITVVAINELADAAGM
                     AHLLKYDTSHGRFAWEVRQERDQLFVGDDAIRVLHERSLQSLPWRELGVDVVLDCTGV
                     YGSREHGEAHIAAGAKKVLFSHPGSNDLDATVVYGVNQDQLRAEHRIVSNASCTTNCI
                     IPVIKLLDDAYGIESGTVTTIHSAMHDQQVIDAYHPDLRRTRAASQSIIPVDTKLAAG
                     ITRFFPQFNDRFEAIAVRVPTINVTAIDLSVTVKKPVKANEVNLLLQKAAQGAFHGIV
                     DYTELPLVSVDFNHDPHSAIVDGTQTRVSGAHLIKTLVWCDNEWGFANRMLDTTLAMA
                     TVAFR"
     gene            6030..7193
                     /gene="pgk"
                     /locus_tag="ACHKBL_001660"
     CDS             6030..7193
                     /gene="pgk"
                     /locus_tag="ACHKBL_001660"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HFO5818541.1"
                     /translation="MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTI
                     ELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVA
                     EGELVVLENVRFNKGEKKDDETLSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
                     ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIA
                     NTFIAAQGHDVGKSLYEADLVDEAKRLLTTCNIPVPSDVRVATEFSETAPATLKSVND
                     VKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKGTEIVANAIADSEA
                     FSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK"
     gene            7407..8486
                     /gene="fbaA"
                     /locus_tag="ACHKBL_001661"
     CDS             7407..8486
                     /gene="fbaA"
                     /locus_tag="ACHKBL_001661"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016505616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HFO5818542.1"
                     /translation="MSKIFDFVKPGVITGDDVQKVFQVAKENNFALPAVNCVGTDSIN
                     AVLETAAKVKAPVIVQFSNGGASFIAGKGVKSDVPQGAAILGAISGAHHVHQMAEHYG
                     VPVILHTDHCAKKLLPWIDGLLDAGEKHFAATGKPLFSSHMIDLSEESLQENIEICSK
                     YLERMSKIGMTLEIELGCTGGEEDGVDNSHMDASALYTQPEDVDYAYTELSKISPRFT
                     IAASFGNVHGVYKPGNVVLTPTILRDSQEYVSKKHNLPHNSLNFVFHGGSGSTAQEIK
                     DSVSYGVVKMNIDTDTQWATWEGVLNYYKANEAYLQGQLGNPKGEDQPNKKYYDPRVW
                     LRAGQTSMIARLEKAFQELNAIDVL"
     gene            8677..9537
                     /gene="mscS"
                     /locus_tag="ACHKBL_001662"
     CDS             8677..9537
                     /gene="mscS"
                     /locus_tag="ACHKBL_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HFO5818543.1"
                     /translation="MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLII
                     ARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGA
                     AGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADG
                     KIIVIPNGKIIAGNIINFSREPVRRNEFIIGVAYDSDIDQVKQILTDIIQSEDRILKD
                     REMTVRLNELGASSINFVVRVWSNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVN
                     FKRVKEDKAA"
     gene            9676..10311
                     /gene="argO"
                     /locus_tag="ACHKBL_001663"
     CDS             9676..10311
                     /gene="argO"
                     /locus_tag="ACHKBL_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0061459 - L-arginine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine exporter ArgO"
                     /protein_id="HFO5818544.1"
                     /translation="MFSYYFQGLALGAAMILPLGPQNAFVMNQGIRRQYHIMIALLCA
                     ISDLVLICAGIFGGSALLMQSPWLLALVTWGGVAFLLWYGFGAFKTAMSSNIELASAE
                     VLKQGRWKIIATMLAVTWLNPHVYLDTFVVLGSLGGQLDVEPKRWFALGTISASFLWF
                     FGLAILAAWLAPRLRTAKSQRIINLVVGCVMWFIALQLARDGIVHAQALLS"
     gene            10404..11144
                     /gene="yggE"
                     /locus_tag="ACHKBL_001664"
     CDS             10404..11144
                     /gene="yggE"
                     /locus_tag="ACHKBL_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative stress defense protein"
                     /protein_id="HFO5818545.1"
                     /translation="MKFKVIALAALMGISGMAAQANELPDGPHIVTSGTASVDAVPDI
                     ATLAIEVNVAAKDAATAKKQADERVAQYISFLELNQIAKKDISSANLRTQPDYDYQDG
                     KSILKGYRAVRTVEVTLRQLDKLNSLLDGALKAGLNEIRSVSLGVAQPDAYKDKARKA
                     AIDNAIHQAQELANGFHRKLGPVYSVRYHVSNYQPSPMVRMMKADAAPVSAQETYEQA
                     AIQFDDQVDVVFQLEPVDQQPAKTPAAQ"
     gene            11312..12208
                     /gene="ygfI"
                     /locus_tag="ACHKBL_001665"
     CDS             11312..12208
                     /gene="ygfI"
                     /locus_tag="ACHKBL_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417396.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO5818546.1"
                     /translation="MDIFISKKMRNFILLAQTNNIARAAEKIHMTASPFGKSIAALEE
                     QIGYTLFTRKDNNISLNKAGQELYQKLFPVYQRLSAIDNEIHNSGRRSREIVIGIDNT
                     YPTIIFDQLISLGDKYEGVTAQPVEFSENGVIDNLFDRQLDFIISPQHVSARVQELEN
                     LTISELPPLRLGFLVSRRYEERQEQELLQELPWLQMRFQNRANFEAMIDANMRPCGIN
                     PTIIYRPYSFMAKISAVERGHFLTVIPHFAWRLVNPATLKYFDAPHRPMYMQEYLYSI
                     RNHRYTATMLQHIAEDRDGTSH"
     gene            complement(12205..13683)
                     /gene="scpC"
                     /locus_tag="ACHKBL_001666"
     CDS             complement(12205..13683)
                     /gene="scpC"
                     /locus_tag="ACHKBL_001666"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA:succinate CoA transferase"
                     /protein_id="HFO5818547.1"
                     /translation="METQWTRMTADEAAEIIQHNDMVAFSGFTPAGSPKALPNAIARR
                     ANEQHEAKKPYQIRLLTGASISAAADDVLSDADAVSWRAPYQTSSGLRKKINQGAVSF
                     VDLHLSEVAQMVNYGFFGDIDVAVIEASALAPDGRVWLTSGIGNAPTWLLRAKKVIIE
                     LNHYHDPRVAELADIVIPGAPPRRNSVSIFHAMDRVGTRYVQIDPKKIVAVVETNLPD
                     AGNMLDKQNPMCQQIADNVVTFLLQEMAHGRIPPEFLPLQSGVGNINNAVMARLGENP
                     EIPPFMMYSEVLQESVVHLLETGKISGASASSLTISADSLRKIYDNMDYFASRIVLRP
                     QEISNNPEIIRRLGVIALNVGLEFDIYGHANSTHVAGVDLMNGIGGSGDFERNAYLSI
                     FMAPSIAKEGKISTVVPMCSHVDHSEHSVKVIITEQGIADLRGLSPLQRARTIIDNCA
                     HPMYRDYLHRYLENAPGGHIHHDLSHVFDLHRNLIATGSMLG"
     gene            complement(13707..14492)
                     /gene="scpB"
                     /locus_tag="ACHKBL_001667"
     CDS             complement(13707..14492)
                     /gene="scpB"
                     /locus_tag="ACHKBL_001667"
                     /EC_number="7.2.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA decarboxylase"
                     /protein_id="HFO5818548.1"
                     /translation="MSYQYVNVVTINKVAVIEFNYGRKLNALSKVFIDDLMQALSDLN
                     RPEIRCIILRAPSGSKVFSAGHDIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISM
                     VEGSVWGGAFEMIMSSDLIIAASTSTFSMTPVNLGVPYNLVGIHNLTRDAGFHIVKEL
                     IFTASPITAQRALAVGILNHVVEVEELEDFTLQMAHHISEKAPLAIAVIKEELRVLGE
                     AHTMNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH"
     gene            complement(14503..15498)
                     /gene="meaB"
                     /locus_tag="ACHKBL_001668"
     CDS             complement(14503..15498)
                     /gene="meaB"
                     /locus_tag="ACHKBL_001668"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HFO5818549.1"
                     /translation="MINEAKLAESIRRLRQGERATLAQAMTLVESRHPRHQALSTQLL
                     DAIMPFCGNALRLGVTGTPGAGKSTFLEAFGMLLIREGLKVAVIAVDPSSPVTGGSIL
                     GDKTRMNDLARAEAAFIRPVPSSGHLGGASQRARELMLLCEAAGYDVVIVETVGVGQS
                     ETEVARMVDCFISLQIAGGGDDLQGIKKGLMEVADLIVINKDDGDNHTNVAIARHMYE
                     SALHILRRKYDEWQPRVLTCSALEKRGIDEIWHAIIDFKTALTASGRLQQVRQQQSVE
                     WLRKQTEEEVLNRLFANEDFDRYYRQTLLAVKNNTLSPRTGLRQLSEFIQTQYFD"
     gene            complement(15491..17635)
                     /gene="scpA"
                     /locus_tag="ACHKBL_001669"
     CDS             complement(15491..17635)
                     /gene="scpA"
                     /locus_tag="ACHKBL_001669"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HFO5818550.1"
                     /translation="MSKVQEWQQLANKELSRREKTVDSLVQQTAEGIAIKPLYTEADL
                     DNLEVTGTLPGLPPYVRGPRATMYTAQPWTIRQYAGFSTAKESNAFYRRNLAAGQKGL
                     SVAFDLATHRGYDSDNPRVAGDVGKAGVAIDTVEDMKVLFDQIPLDKMSVSMTMNGAV
                     LPVLAFYIVAAEEQGVTPDKLTGTIQNDILKEYLCRNTYIYPPKPSMRIIADIIAWCS
                     GNMPRFNTISISGYHMGEAGANCVQQVAFTLADGIEYIKAAISAGLKIDDFAPRLSFF
                     FGIGMDLFMNVAMLRAARYLWSEAVSGFGAQDPKSLALRTHCQTSGWSLTEQDPYNNV
                     IRTTIEALAATLGGTQSLHTNAFDEALGLPTDFSARIARNTQIIIQEESELCRTVDPL
                     AGSYYIESLTDQIVKQARAIIQQIDEAGGMAKAIEAGLPKRMIEEASAREQSLIDQGK
                     RVIVGVNKYKLDHEDETDVLEIDNVMVRNEQIASLERIRATRDDAAVTAALNALTHAA
                     QHNENLLAAAVNAARVRATLGEISDALEAAFDRYLVPSQCVTGVIAQSYHQSEKSAAE
                     FDAIVAQTEQFLADNGRRPRILIAKMGQDGHDRGAKVIASAYSDLGFDVDLSPMFSTP
                     EEIARLAVENDVHVVGASSLAAGHKTLIPELVEALKKWGRKDICVVAGGVIPPQDYAF
                     LQERGVAAIYGPGTPMLDSVRDVLNLISQHHD"
     gene            complement(17839..18732)
                     /gene="argP"
                     /locus_tag="ACHKBL_001670"
     CDS             complement(17839..18732)
                     /gene="argP"
                     /locus_tag="ACHKBL_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator ArgP"
                     /protein_id="HFO5818551.1"
                     /translation="MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLEN
                     MFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSL
                     ATWLLPALAPVLADSPIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLG
                     ALDYLFVSSKPFAEKYFPNGVTRSALLKAPVVAFDHLDDMHQAFLQQNFDLPPGSVPC
                     HIVNSSEAFVQLARQGTTCCMIPHLQIEKELASGELIDLTPGLFQRRMLYWHRFAPES
                     RMMRKVTDALLDYGHKVLRQD"
     gene            18873..19058
                     /locus_tag="ACHKBL_001671"
     CDS             18873..19058
                     /locus_tag="ACHKBL_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001328908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5818552.1"
                     /translation="MHFAQRVRALVVLNGVALLPLFASWSGIPRPLYALFAGRKGMPA
                     IARYFMDELTTRLANGV"
     gene            19114..19773
                     /gene="rpiA"
                     /locus_tag="ACHKBL_001672"
     CDS             19114..19773
                     /gene="rpiA"
                     /locus_tag="ACHKBL_001672"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HFO5818553.1"
                     /translation="MTQDELKKAVGWAALQYVQPGTIVGVGTGSTAAHFIDALGTMKG
                     QIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTR
                     EKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGV
                     VTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIV
                     K"
     gene            20029..21261
                     /gene="serA"
                     /locus_tag="ACHKBL_001673"
     CDS             20029..21261
                     /gene="serA"
                     /locus_tag="ACHKBL_001673"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HFO5818554.1"
                     /translation="MAKVSLEKDKIKFLLVEGVHQKALESLRAAGYTNIEFHKGALDD
                     EQLKESIRDAHFIGLRSRTHLAEDVINAAEKLVAIGCFCIGTNQVDLDAAAKRGIPVF
                     NAPFSNTRSVAELVIGELLLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYG
                     HIGTQLGILAESLGMYVYFYDIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTK
                     NMMGAKEISLMKPGSLLINASRGTVVDIPALCDALASKHLAGAAIDVFPTEPATNSDP
                     FTSPLCEFDNVLLTPHIGGSTQEAQENIGLEVAGKLIKYSDNGSTLSAVNFPEVSLPL
                     HGGRRLMHIHENRPGVLTALNKIFAEQGVNIAAQYLQTSAQMGYVVIDIEADEDVAEK
                     ALQAMKAIPGTIRARLLY"
     gene            21490..21549
                     /gene="ibsC"
                     /locus_tag="ACHKBL_001674"
     CDS             21490..21549
                     /gene="ibsC"
                     /locus_tag="ACHKBL_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsC"
                     /protein_id="HFO5818555.1"
                     /translation="MMRLVIILIVLLLISFPAY"
     gene            complement(21650..22252)
                     /gene="fau"
                     /locus_tag="ACHKBL_001675"
     CDS             complement(21650..22252)
                     /gene="fau"
                     /locus_tag="ACHKBL_001675"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HFO5818556.1"
                     /translation="MTQLPELPLTLSRQEIRKMIRQRRRALTPEQQQEMGQQAATRMM
                     TYPPVVMAHTVAVFLSFDGELDTLPLIEQLWRAGKRVYLPVLHPFSAGNLLFLNYHPQ
                     SELVMNRLKIHEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQ
                     HYKMQPVGYAHDCQLVEKLPVEEWDIPLPAVVTPSKVWEW"
     gene            complement(22273..22456)
                     /gene="ssrS"
                     /locus_tag="ACHKBL_001676"
     ncRNA           complement(22273..22456)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ACHKBL_001676"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(22498..22827)
                     /gene="zapA"
                     /locus_tag="ACHKBL_001677"
     CDS             complement(22498..22827)
                     /gene="zapA"
                     /locus_tag="ACHKBL_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFO5818557.1"
                     /translation="MSAQPVDIQIFGRSLRVNCPPDQRDALNQAADDLNQRLQDLKER
                     TRVTNTEQLVFIAALNISYELAQEKAKTRDYAASMEQRIRMLQQTIEQALFEQGRITE
                     KTNQNFE"
     gene            22995..23573
                     /gene="ygfB"
                     /locus_tag="ACHKBL_001678"
     CDS             22995..23573
                     /gene="ygfB"
                     /locus_tag="ACHKBL_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YgfB"
                     /protein_id="HFO5818558.1"
                     /translation="MSIQNEMPGYNEMNQYLNQQGTGLTPAEMHGLISGMICGGNDDS
                     SWLPLLHDLTNEGMAFGHELAQALRKMHSATSDALQDDGFLFQLYLPDGDDVSVFDRA
                     DALAGWVNHFLLGLGVTQPKLDKVTGETGEAIDDLRNIAQLGYDEDEDQEELEMSLEE
                     IIEYVRVAALLCHDTFTHPQPTAPEVQKPTLH"
     gene            23599..24924
                     /gene="pepP"
                     /locus_tag="ACHKBL_001679"
     CDS             23599..24924
                     /gene="pepP"
                     /locus_tag="ACHKBL_001679"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HFO5818559.1"
                     /translation="MSEISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPY
                     RQNSDFWYFTGFNEPEAVLVLIKSDDTHNHSVLFNRVRDLTAEIWFGRRLGQDAAPEK
                     LGVDRALAFSEINQQLYQLLNGLDVVYHAQGEYTYADEIVNSALEKLRKGSRQNLTAP
                     ATMIDWRPVVHEMRLFKSPEEIAVLRRAGEITALAHTRAMEKCRPGMFEYHLEGEIHH
                     EFNRHGARYPSYNTIVGSGENGCILHYTENECELRDGDLVLIDAGCEYKGYAGDITRT
                     FPVNGKFTQAQREIYDIVLESLETSLRLYRPGTSMQEVTGEVVRIMVSGLVKLGILKG
                     DVDELIAQNAHRPFFMHGLSHWLGLDVHDVGVYGQDRSRILEPGMVLTVEPGLYIAPD
                     ADVPEQYRGIGIRIEDDIVITETGNENLTASVVKKPEEIEALMAAARKQ"
     gene            24921..26099
                     /gene="ubiH"
                     /locus_tag="ACHKBL_001680"
                     /gene_synonym="visB"
     CDS             24921..26099
                     /gene="ubiH"
                     /locus_tag="ACHKBL_001680"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008681 - 2-octaprenyl-6-methoxyphenol
                     hydroxylase activity [Evidence IEA];
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HFO5818560.1"
                     /translation="MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPG
                     FDGRAIALAAGTCQQLARIGVWQSLADCATAITTVHVSDRGHAGFVTLAAEDYQLAAL
                     GQVVELHNVGQRLFALLRKAPGVTLHCPDRVANVARTQSHVEVTLESGETLTGCVLVA
                     ADGTHSALATVCGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTQYGPLAMLPMSDG
                     RCSLVWCHPLERREEVLSWSDEKFCRELQSAFGWRLGKITHAGKRSAYPLALTRAARP
                     ITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAQERGEDMGDYGVLCRYQQ
                     RRQRDREATIGVTDSLVHLFANRWGPLVVGRNIGLMAMELFTPARDVLAQRTLGWVAR
                     "
     gene            26122..27324
                     /gene="ubiI"
                     /locus_tag="ACHKBL_001681"
                     /gene_synonym="visC"
     CDS             26122..27324
                     /gene="ubiI"
                     /locus_tag="ACHKBL_001681"
                     /gene_synonym="visC"
                     /EC_number="1.14.13.240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent 2-octaprenylphenol hydroxylase"
                     /protein_id="HFO5818561.1"
                     /translation="MQSVDVAIVGGGMVGLAVACGLQGSGLRVAVLEQRVPEPLAADA
                     PPQLRVSAINAASEKLLTRLGVWQDILSRRASCYHGMEVWDKDSFGHISFDDQSMGYS
                     HLGHIVENSVIHYALWNKAQQSSDITLLAPAELQQVAWGENETFLTLKDGSMLTARLV
                     IGADGANSWLRNKADIPLTFWDYQHHALVATIRTEEPHDAVARQVFHGEGILAFLPLS
                     DPHLCSIVWSLSPEEAQRMQQAGEDEFNRALNIAFDNRLGLCKVESERLVFPLTGRYA
                     RQFAAHRLALVGDAAHTIHPLAGQGVNLGFMDAAELIAELKRLHRQGKDIGQYIYLRR
                     YERSRKHSAALMLAGMQGFRDLFSGTNPAKKLLRDIGLKLADTLPGVKPQLIRQAMGL
                     NDLPEWLR"
BASE COUNT         6540 a         6733 c         7342 g         6795 t
ORIGIN      
        1 atcaatctca gttatacatt cagcaaaaga ttggcaggaa gctatcgatt tctcgatggc
       61 atcattgctg gataaaaact atatcagcga gaattacatt caggcaatta aagattccac
      121 cattaacaat ggcccttatt atattctcgc accaggcgtg gcaatgcctc atgctcgacc
      181 ggaatgtggg gcgcttaaaa ccgggatgtc tttaacatta cttaaacaag gcgtttattt
      241 tccggggaat gacgaaccga taaaattact catcgggctt tctgctgccg atgccgattc
      301 gcacattggc gctattcagg cgttaagtga attattgtgt gaagaagaaa tactcaacca
      361 attattaaca gcagcatcag aaaaacaatt agcggacata atcagccgcg gataattact
      421 ctctcctcta caggataata ttatggaaaa caagtctgct cgtgcaaagg tccaggcttt
      481 tgggggcttt ttgactgcaa tggtcatccc caatatcggc gcttttatag cctggggttt
      541 tataacggcg ttatttattc ccaccggctg gctgcctaac gaacatttcg ccaaaattgt
      601 cggcccgatg attacctatt tattgcccgt gatgattggt tctaccggtg gtcatctggt
      661 cggcggtaaa cgcggcgcgg tcatgggcgg aataggtact attggtgtga tcgtcggcgc
      721 agagatcccg atgttccttg gctcaatgat tatggggccg ctcggtggtc tggtgattaa
      781 atatatcgac aaggcgctgg aaaagcggat ccctgctggt tttgagatgg ttatcaataa
      841 cttctcatta ggtattgcgg ggatgctcct ttgtctgctg ggttttgaag ttatcggccc
      901 ggcggtgtta attgccaata ctttcgtcaa agagtgtatt gaggcgttgg tacatgcggg
      961 ttatctgcct ttgctgtcag tcatcaatga accggcgaaa gtgcttttcc tcaataacgc
     1021 gatagatcag ggcgtctatt acccgttggg gatgcaacag gcttcagtta acggtaaatc
     1081 catcttcttt atggtggcgt ccaaccctgg gcctggctta ggactgttgc tggcgtttac
     1141 tttgtttggt aaagggatga gtaaacgttc tgcgcccggg gcgatgatta ttcacttcct
     1201 cggtgggatc cacgaactgt atttcccgta tgtgctgatg aagccgctga ccattattgc
     1261 catgattgcg ggcggtatgt ctggcacctg gatgtttaac ttactggacg gtggtctggt
     1321 ggctggccca agtccggggt ctatctttgc ttacctggca ctgacgccga aaggctcgtt
     1381 cctggcgaca attgccggtg ttacggtagg taccctggtg tcctttgcta tcacttccgt
     1441 aatactgaag atggaaaaaa cggtggaaac ggagagcgaa gatgagtttg ctcagtcagc
     1501 caatgcggtt aaggcgatga aacaagaagg tgcattctcg ttaagcaggg ttaagcgtat
     1561 cgcctttgtt tgcgatgcgg ggatgggctc cagcgcgatg ggcgcgacca ccttccgtaa
     1621 acgcctggaa aaggcggggc tggcaataga agtaaaacat tacgcgatag aaaacgtgcc
     1681 tgcggatgcg gatatcgttg ttactcatgc cagtctggaa gggcgcgtga aacgtgtgac
     1741 ggataaaccg ctgatactga ttaataacta tattggcgat ccaaaactcg acactttatt
     1801 taatcaatta accgccgaac ataaaaactg attgccgagg taaaaatgaa aaccaaagtt
     1861 gctgctattt atggcaagcg ggatgtccgt ctgcgcgaat ttgaactgcc agaaattacc
     1921 gataatgaac tgctggtgag tgtgatttct gacagcgtgt gtttatccac ctggaaagcg
     1981 gcgctactcg gtagtgaaca taaacgcgta cccgacgatt tagaaaatca tccggtcatt
     2041 accgggcatg aatgcgccgg ggttattgtc gaagtgggta aaaatctcac cgacaaatat
     2101 aaaaaaggcc agcgttttgt attgcaaccg gcaatggggt taccaagcgg atattcagca
     2161 ggctacagct acgaatattt tggcggtaac gccacctata tgattattcc tgaaatagcc
     2221 attaacctgg gctgcgtatt accgtatcac ggctcttatt ttgctgcggc gtcgctggca
     2281 gagcctatgt gctgcattat tggcgcttat catgccaatt atcacaccac gcaatatgtt
     2341 tatgagcatc gcatgggcgt taaacctggc ggcaatattg cgctgctggc atgtgctggt
     2401 ccgatgggca ttggcgctat cgattatgct attaacggcg gcatacaacc gtcgcgggtg
     2461 gtggtggtcg atatcgacga caaacgcctg gcgcaggtac aaaagctgct gccggtggaa
     2521 ctggcggcca gtaaaggcat tgagctggtg tatatgaata ccaaagggat gagcgatccg
     2581 gtccagacgc tgcgggcgct gacggatgat gccggatttg atgacgtatt tgtctatgca
     2641 gcggtgcctt cagtcgtcga gatggctgat gaattactgg cggaagatgg ctgcctgaac
     2701 ttctttgccg ggccgacgga taaaaacttc aaagtgccgt ttaatttcta caacgtccat
     2761 tacaacagca cgcatgtcgt cggtacgtcc ggcggttcaa cggacgacat gaaagaggcg
     2821 attgcgctta gcgccacagg gcagttgcag ccgtcgttta tggtgaccca tatcggtggg
     2881 ctggatgcgg tgcctgatac cgtgctcaat ctgccggata tccccggcgg taaaaaactc
     2941 atttataacg gcgtcaccat gccgctcact gccattgccg attttgccga aaaaggcaaa
     3001 accgatccgc tgtttaaaga gctggcgcgg ctggttgagg aaacgcacgg catctggaat
     3061 gagcaggccg agaaatatct gctggcgcaa tttggcgttg atatcgggga ggccgcgcaa
     3121 tgatgtccct ggcgtggcca ttatttcgcg ttacggaaca ggcagcgttg gctgcctggc
     3181 cgcaaaccgg atgtggcgac aaaaataaaa ttgatggcct ggcggtcacc gcgatgcgcc
     3241 aggcattaaa cgacgtcgct tttcgtgggc gagtggttat cggcgagggg gaacgatatc
     3301 ctctttagcg ctacgggcgt gacgggcggc gagttggtga acggtatcca acagacggcg
     3361 aatggggtgc ggacgcagac attactgatc ggcggcgcgg accaaacgtg taatataata
     3421 gactccctgc attgatggtg atctatcgga agaccaatgg cgacgctgac agaagatgat
     3481 gtgcttgagc aactggatgc acaggacaat ttattttcat ttatgaaaac tgcgcattct
     3541 attttgctcc aggggatacg ccagtttctg ccgtcgctgt ttgtcgataa cgatgaagag
     3601 atcgtcgaat atgcagtgaa gccgttactc gcccaaagcg gcccgcttga cgatattgat
     3661 gttgcgctgc gtttgattta tgcgctgggg aaaatggata aatggctgta cgccgatatc
     3721 acgcattttt cccagttttg gcattacctg aacgaacagg atgaaacgcc cggatttgcc
     3781 gatgacatga cctgggattt tatcagcaac gtcaatagca taacctgcaa tgcgacgctc
     3841 tatgacgcgt tgaaagcgat gaagttcgcc gatttcgccg tctggtcaga ggcgcgtttt
     3901 agcaggatgg tcaaaacggc gctgacgctg gcagtaacga ccaccttaaa ggaattaacg
     3961 ccgtgaaaat tgaattaacg gtgaatgggc tgaagataca ggcacagtac cagaacgaag
     4021 agattgaaaa tgtacataaa ccgttgctgc acatgttggc ggccttacag acggtaaatc
     4081 cgcagcggcg tacggtggtt tttctttgcg ctccgccggg gacaggtaaa tctaccctaa
     4141 ccaccttctg ggaatatctc gcacaacaag acccggaact gcccgctatt cagacactgc
     4201 cgatggatgg ttttcaccat tacaatagct ggctggatgc gcatcaattg cgccccttca
     4261 aaggcgcacc agagacattc aacgttgcga aactggcgga aaatctgtgc cgggttgtgg
     4321 aaggggattg tacgtggccg cagtacgatc gacaaaagca tgatcctgtt gaagatgcgt
     4381 tgcacgttac cgcaccactc gtcatcgtcg aaggaaactg gctattactg gatgatgaaa
     4441 agtggcagga gttggcatct ttctgcgatt tttctatttt tattcatgca cctgctcaaa
     4501 ttctgcggga gcgcttgatt agtcgtaaaa ttgcgggagg attatcgcgg caagaagccg
     4561 aagcttttta tgcccgaacc gatggtccca atgtcgagcg tgtgctggtg aatagtcgtc
     4621 aggccaatct gatattagag atgaccgaag aaggcagata tcacttcaaa agctgacaat
     4681 ttattccagt tattgcaggg gtattattac gccagcctgg aataaagccc cctacaaaag
     4741 gggataaaag tgtgatgtaa gtcagataaa tgtcttcttc ggctggacaa tcattccttt
     4801 tattccacgt ttcgcttatc ctagctgaag cgtttcagtc gattaaatgt tcgacaatta
     4861 accaatcagt cgcagtttgc gacaggtaag gtttccccgg acgatttgct ggattactct
     4921 gtctggcaaa tttgctcagg gaaaaccttg caggagatct atgaccgtac gcgtagcgat
     4981 aaatggcttc ggtcgcatcg ggcgtaatgt ggttcgtgct ttgtatgaat ccggacgccg
     5041 ggcggaaatt accgtggtgg caatcaacga actggcggat gctgcgggca tggcgcattt
     5101 gttgaaatat gacaccagcc atggccgttt tgcatgggaa gtacgacagg aacgcgatca
     5161 actttttgtt ggtgatgacg ccatccgtgt attgcatgaa cgttcactgc aatcgctccc
     5221 ctggcgtgaa cttggcgttg atgtagtcct cgactgcacc ggcgtatatg gctcccgcga
     5281 gcatggcgaa gcgcatattg ccgccggggc caaaaaagtg ctcttttcac atccaggcag
     5341 taacgatctc gacgcgaccg ttgtttacgg cgtcaatcag gatcaacttc gtgcggaaca
     5401 ccgcatcgtt tctaacgctt cctgtaccac gaattgcata attcccgtca tcaaattgtt
     5461 agatgatgcg tacggtattg agtccggcac tgtgaccaca attcactccg ccatgcacga
     5521 tcaacaggtt attgatgcat accatcctga cctgcgtcgc acccgggctg ccagccagtc
     5581 gatcattccg gtcgatacta aactggccgc cggtatcaca cgattttttc cgcaatttaa
     5641 cgatcgcttt gaagcgattg cggtacgtgt gccaaccata aatgtgacgg caatcgattt
     5701 aagcgtgacg gtgaagaaac ctgtaaaagc caatgaagtc aacctgttgc tgcaaaaagc
     5761 agcacaaggt gcatttcatg gtatagttga ctatacggaa ttgccgttgg tctctgtaga
     5821 ttttaaccac gatccgcaca gtgccattgt cgatggcacc caaacccggg ttagtggcgc
     5881 acacctgatc aaaacgttgg tctggtgcga taacgaatgg ggctttgcta accgaatgct
     5941 cgacacgacg ttagctatgg ctactgttgc tttcaggtaa gacgcaagca gcgtctgcaa
     6001 aacttttaga atcaacgaga ggattcacca tgtctgtaat taagatgacc gatctggatc
     6061 ttgctgggaa acgtgtattt atccgtgcgg atctgaacgt accagtaaaa gacgggaaag
     6121 taaccagcga cgcgcgtatc cgtgcttctc tgccgaccat cgaacttgcc ctgaaacaag
     6181 gcgcaaaagt gatggtaact tcccacctgg gtcgtcctac cgaaggcgag tacaacgaag
     6241 aattctctct gctgccggtt gttaactacc tgaaagacaa actgtctaac ccggttcgtc
     6301 tggttaaaga ttacctcgac ggcgttgacg ttgctgaagg tgaactggtt gttctggaaa
     6361 acgttcgctt caacaaaggc gagaagaaag acgacgaaac cctgtccaaa aaatacgctg
     6421 cactgtgtga cgtgttcgta atggacgcat tcggtactgc tcaccgcgcg caggcttcta
     6481 ctcacggtat cggtaaattc gctgacgttg cgtgcgcagg cccgctgctg gcagctgaac
     6541 tggacgcgct gggtaaagca ctgaaagaac ctgctcgccc gatggtggct atcgttggtg
     6601 gttctaaagt atctaccaaa ctgaccgttc tggactccct gtctaaaatc gctgaccagc
     6661 tgattgttgg tggtggtatc gctaacacct ttatcgcggc acaaggccac gatgtgggta
     6721 aatccctgta cgaagctgat ctggttgacg aagcaaaacg tctgctgacc acctgcaaca
     6781 tcccggttcc gtctgatgtt cgtgtagcaa ctgagttctc tgaaactgca ccggctaccc
     6841 tgaaatctgt taacgatgtg aaagctgacg agcagatcct ggatatcggt gatgcttccg
     6901 ctcaggaact ggctgaaatc ctgaagaatg cgaaaaccat tttgtggaac ggtccggttg
     6961 gcgtgttcga attcccgaac ttccgcaaag gtactgaaat cgtggctaat gctatcgcag
     7021 acagcgaagc gttctccatc gctggcggcg gcgacactct ggcagcaatc gacctgttcg
     7081 gcattgctga caaaatctcc tacatctcca ctggcggcgg tgcattcctc gaattcgtgg
     7141 aaggtaaagt actgcctgca gtagcgatgc tcgaagagcg cgctaagaag taaaaaatca
     7201 cagggcaggg aaacctgccc ttgttgcagc gcgcttttaa agcacgcact ttttcatact
     7261 ctaaataatt cgagttgcag gaaggcgaca agagagtgaa tcccatgagc ttacataagt
     7321 aagtgactgg ggtgagcgaa cgcagacgca gcacatgcaa cttgaagtat gacgagtata
     7381 aggcccgacg atacaggaca agagacatgt ctaagatttt tgatttcgta aaacctggcg
     7441 taatcactgg tgatgacgta cagaaagttt tccaggtagc aaaagaaaac aacttcgcac
     7501 tgccagcagt aaactgcgtc ggtactgact ccatcaacgc cgtgctggaa accgctgcta
     7561 aagttaaagc gccggttatc gttcagttct ccaacggtgg tgcttccttt atcgctggta
     7621 aaggcgtgaa atctgacgtt ccgcagggtg ctgctatcct gggcgcgatc tctggtgcgc
     7681 atcacgttca ccagatggct gaacattatg gtgttccggt tatcctgcac accgaccact
     7741 gcgcgaagaa actgctgccg tggatcgacg gtctgctgga cgcgggtgaa aaacacttcg
     7801 cagctaccgg caagccgctg ttctcttctc acatgatcga cctgtctgaa gaatctctgc
     7861 aagagaacat cgaaatctgc tctaaatacc tggagcgcat gtccaaaatc ggcatgactc
     7921 tggaaatcga actgggttgc accggtggtg aagaagacgg cgtggacaac agccacatgg
     7981 acgcttctgc actgtacacc cagccggaag acgttgatta tgcgtatacc gagctgagca
     8041 aaatcagccc gcgtttcacc atcgcagcgt ccttcggtaa cgtacacggt gtttacaagc
     8101 cgggtaacgt ggttctgact ccgaccatcc tgcgcgactc tcaggaatat gtttctaaga
     8161 aacacaacct gccgcacaac agcctgaact tcgtattcca cggtggttcc ggttctactg
     8221 ctcaggaaat caaagactcc gtaagctacg gcgtagtgaa aatgaacatc gataccgata
     8281 cccaatgggc aacctgggaa ggcgttctga actactacaa agcgaacgaa gcttatttgc
     8341 agggtcagct gggtaacccg aaaggcgaag atcagccgaa caagaaatac tacgatccgc
     8401 gcgtatggct gcgtgccggt cagacttcga tgatcgctcg tctggaaaaa gcattccagg
     8461 aactgaacgc gatcgacgtt ctgtaagtca ttcctttctg cttatctcaa ggcccgctct
     8521 gcgggtcttt ttttcgccaa aagcaaggtg atgcagatga gaaatctgtg atctatttgg
     8581 caaaattatg ctttattgtt tacccttgtc agactgcccg ccataaggcg gcggagtgta
     8641 tttctccatt ttgagtcagt tgaaaaggaa tattgaatgg aagatttgaa tgttgtcgat
     8701 agcataaacg gcgcgggaag ctggctggtg gctaaccagg cgctgctgct aagttatgca
     8761 gtaaacatcg tggcggcact cgcgatcatc attgttggtt tgattatcgc gcggatgatt
     8821 tccaacgcgg tgaatcgcct gatgatctcc cgtaaaatcg atgccactgt tgctgatttt
     8881 ctctctgcat tagtccgtta cggtattatc gcctttacgc taatcgctgc actggggcgc
     8941 gtgggcgtac aaacagcgtc agtcattgct gtactcggtg ccgcaggctt agcggttggt
     9001 ctggctttgc aggggtcact gtctaacctg gccgctggcg tgttacttgt tatgttccgc
     9061 ccgttccgtg ccggagaata tgttgatctc ggcggcgtag ccggtactgt gctgagtgtg
     9121 cagattttct ccaccaccat gcgtactgca gacggtaaaa ttatcgttat tccgaacggt
     9181 aaaattattg ccggaaatat tattaacttc tctcgcgagc cagttcgccg taacgaattt
     9241 attattggcg tggcgtatga ttccgatatc gatcaggtta agcagatcct gaccgatatt
     9301 atccagtctg aagatcgcat tttgaaagat cgcgaaatga ctgtgcgcct gaacgaactt
     9361 ggtgcatcgt cgattaattt cgtggtccgc gtctggagca acagcggcga tctgcaaaac
     9421 gtgtactggg atgtgctgga gcgtattaaa cgtgaatttg atgccgccgg tatcagcttc
     9481 ccgtacccgc aaatggatgt gaactttaag cgggtgaaag aagacaaagc tgcgtaatca
     9541 acgctgaggc cagataatac tctctggcct ctctcttatt agtttttctg attgccaatt
     9601 aatattatca atttccgcta ataacaatcc cgcgatatag tctctgcatc agatacttaa
     9661 ttcggaatat ccaacgtgtt ttcttattac tttcaaggtc ttgcacttgg ggcggctatg
     9721 atcctaccgc tcggtccaca aaatgctttt gtgatgaatc agggcattcg tcgtcagtac
     9781 cacattatga ttgccttact ttgcgctatc agcgatttgg tcctgatttg cgccgggatt
     9841 tttggtggca gcgcgttatt gatgcagtcg ccgtggttgc tggcgctggt cacctggggc
     9901 ggcgtagcct tcttgctgtg gtatggtttt ggcgctttta aaacagcgat gagcagtaat
     9961 attgagttag ccagtgccga agtcctgaag caaggcagat ggaaaattat cgccaccatg
    10021 ctggcagtga cctggctgaa tccgcatgtt tacctggata cttttgttgt actgggcagt
    10081 cttggcgggc aacttgatgt ggaaccaaaa cgttggtttg cgctcgggac aattagcgcc
    10141 tctttcctgt ggttctttgg tctggctatt ctcgcagcct ggctggcacc gcgcctgcgc
    10201 acggcaaaat cacagcgcat tatcaatctg gtagtgggat gtgttatgtg gtttattgcc
    10261 ttgcagctgg cgagagacgg tattgttcat gcacaagcct tgctcagtta ggcgctatct
    10321 gatggaaaaa taaaacagag gcgctaagct tgcctccaga ggtcctgaat tttaggcaag
    10381 tcaattaaca cggagagact aacgtgaagt tcaaagttat cgccctggcg gcattaatgg
    10441 gtattagcgg gatggcagcg caggctaacg aattgccgga tggaccgcat attgtcacct
    10501 ccggtacggc aagcgtggat gcggtgccag acattgccac tcttgcgatt gaagtcaacg
    10561 tggccgcgaa ggatgccgct actgccaaga aacaggcgga tgagcgcgtc gcacaataca
    10621 tttccttcct tgaactcaat cagatcgcga aaaaagatat cagctcagcg aacttacgca
    10681 cccagccaga ttatgattat caggatggta aaagtatcct gaaaggctac cgtgctgtga
    10741 gaacggtgga agtcacgctc cgtcagttag acaaactgaa ttccttgctg gatggcgcgc
    10801 tgaaggcggg tcttaacgaa attcgttctg tatcgctggg cgtggcgcag ccggatgcct
    10861 ataaagacaa agcgcgtaag gcagcgattg ataacgcgat tcatcaggcg caggaactgg
    10921 cgaacggctt tcatcgtaaa ctggggccgg tatatagtgt gcgctaccat gtttccaact
    10981 atcagcccag cccaatggtg cggatgatga aagccgatgc cgcgccggtg tccgcccagg
    11041 aaacttacga gcaggccgct attcagtttg atgatcaggt cgatgtggtc ttccagttag
    11101 aacctgtgga tcaacaaccc gctaaaacac ctgcagcaca ataatatttg tagcccgacg
    11161 tattcgtcgg gctaatgcct ttacccgata ttgcctcctt tttctgtaat tttctgtaaa
    11221 accatgacgt aatcacctat ctttgctcgc ggtttcatca aggataggct gactgaaacg
    11281 gttaagcgga caaggatgcg ggggaggggg tatggacatt tttatttcga aaaagatgcg
    11341 caatttcatt ttattagcgc aaaccaataa tattgcccgg gcggcagaaa aaatccatat
    11401 gacagcttcg ccatttggta aaagcattgc cgcgctggaa gaacaaattg gctatacgct
    11461 atttacccgc aaagataata atatcagcct caataaagcc ggacaggaac tgtatcaaaa
    11521 actgttcccg gtttatcaac ggctttctgc gattgataat gaaatccata actcagggcg
    11581 tcgttcacga gaaattgtga ttggcataga taatacttat ccgacaatta ttttcgatca
    11641 gttgattagt ctcggagata agtatgaagg cgtgaccgcc cagccagttg agttcagcga
    11701 aaatggcgtt atcgataatc tttttgaccg tcagttagat tttattatct ccccacagca
    11761 tgtatctgca cgagttcagg aactggaaaa cctgactatc agtgaactac cgccgctgcg
    11821 attgggcttt ctcgtttctc gtcgttatga agagcgacaa gagcaagagt tgcttcagga
    11881 gctaccctgg ctacaaatgc gctttcagaa ccgggctaac ttcgaagcaa tgattgatgc
    11941 gaatatgcgt ccttgtggga tcaatcccac cattatttat cgtccctaca gttttatggc
    12001 caaaatcagc gccgtggagc gcggacattt tctgacggtg atcccgcatt ttgcctggcg
    12061 actggtgaat ccggcaacgc tgaaatattt tgatgcgccg cacaggccga tgtatatgca
    12121 ggaatatctt tattcaatca gaaatcatcg ttataccgcc acgatgcttc agcatattgc
    12181 tgaagatcgt gacgggacga gtcattaacc cagcatcgag ccggttgcaa ttaaattacg
    12241 gtgtaagtcg aagacgtggc taagatcgtg gtgaatatgt ccgccgggcg cattttccag
    12301 atagcgatgc agataatccc gatacatagg atgtgcacaa ttatcaatga tagtgcgggc
    12361 gcgttgaagc ggggaaagac cacgcagatc ggcgatgcct tgttcggtga tgatcacttt
    12421 gacgctgtgt tcgctgtgat caacatggct gcacattggc acgacggttg agatcttgcc
    12481 ttctttagca atcgacgggg ccataaagat cgacagatac gcgttgcgtt caaaatcacc
    12541 gctgccaccg atgccgttca tcagatcgac cccggctacg tgcgttgagt tggcatgccc
    12601 gtaaatatca aactccaggc cgacgttcag agcgatgacg cccagacgac ggatgatttc
    12661 cgggttattg gaaatctcct gcggacgcaa cacaatgcgg ctggcaaagt aatccatatt
    12721 gtcgtaaatc ttgcgcaggg aatcggccga gatagtcagg ctggaggcgc tggccccgct
    12781 gattttgccc gtttccagta aatgcaccac cgattcctgt agcacttccg aatacatcat
    12841 aaacggagga atttccggat tttcccccag acgcgccatt accgcattat tgatgttgcc
    12901 tacgccactt tgcagcggca gaaattccgg cggaatacgc ccatgcgcca tttcctgcaa
    12961 caagaacgtg accacgttat cggcaatctg ctggcacatg ggattttgct tatccagcat
    13021 attaccggcg tcaggcaagt tggtttccac gacggcgaca atctttttcg gatcgatttg
    13081 cacatagcgg gtaccgacgc gatccattgc atggaagatc gacacgctat tgcgccgcgg
    13141 tggcgcgcca ggaatcacaa tatctgccag ttctgcaacg cgcggatcgt gatagtgatt
    13201 gagttcaatg atcactttct tcgcccgcag cagccaggtc ggcgcattac cgatcccgct
    13261 ggttaaccag actcgaccat ccggtgccag tgccgatgct tcaatgacgg caacatcaat
    13321 gtcgccgaag aaaccgtaat tgaccatttg cgccacttcg ctcaaatgca ggtcaacgaa
    13381 actcaccgcg ccctgattga tctttttacg taaaccggac gatgtttgat atggcgcacg
    13441 ccaggaaaca gcatcggcgt cagaaagtac atcgtcagcg gcggcgctga ttgacgcccc
    13501 cgtcagcagg cgaatttgat acggcttttt ggcctcatgc tgttcgttag ctctgcgggc
    13561 aatcgcgttg ggtaaggctt tcggcgaacc cgctggggta aagccgctaa atgccaccat
    13621 gtcgttatgc tggataattt ctgccgcttc atcggcggtc atccttgtcc actgagtttc
    13681 cattttaact ccttcgttcg cagggattaa tgaccaacga aattaggttt acgtttttcg
    13741 aggaaagcgt tcatcccttc ctggtaatct tcgctgtcat aaaccgcgcg gcgcatcccc
    13801 tgaatacgtt caaattcatc ggagttcatg gtgtgcgctt cgcccagtac acgcagctct
    13861 tctttgataa cggcaatggc taacggcgct ttctcagaga tgtggtgcgc catttgtaag
    13921 gtgaaatctt ccagttcttc cacttccaca acatggttga ggatgccgac agccagcgcg
    13981 cgctgggctg tgattggcga agcggtaaaa atcagctctt tgacaatgtg gaagcccgcg
    14041 tcgcgggtca ggttgtgaat gccgaccagg ttatacggga cgccgaggtt tacaggtgtc
    14101 attgagaagg ttgaggtact ggcggcgatg atcagatcgg aactcatgat catttcaaat
    14161 gcgccacccc agacactacc ttccaccatc gaaatgatcg gtttcgggaa tttttggatc
    14221 atgcgggtga tttgacgcaa cggatcatca taggagagcg gatcgcgacc gccagacggc
    14281 agttcgtgaa tatcgtgacc tgcggagaag actttggaac cactcggcgc gcgcaaaatg
    14341 atacagcgaa tttccggccg gttgagatcg cttaatgcct gcataagatc atcaataaag
    14401 actttactta aggcattaag tttgcggcca tagttaaact caatgaccgc cactttgttg
    14461 atagtgacaa cgttcacata ctgataagac ataaaaattc ctttaatcaa aatattgcgt
    14521 ctggataaat tcactgagct gccgcaggcc ggtgcgcggt gagagcgtat tgtttttgac
    14581 tgctaaaagc gtctggcggt aatagcgatc gaaatcttca ttcgcgaaca ggcgattcag
    14641 tacttcttct tcggtctgct tacgcagcca ttccaccgat tgttgttgcc gcacttgttg
    14701 taatcgacca ctggcagtta gcgcggtttt gaagtcgatg atggcgtgcc agatctcatc
    14761 gattccacgt ttttccagtg cgctacaagt cagaacccgt ggctgccatt cgtcgtattt
    14821 acgtcgcaga atatgcaggg cactctcgta catatgccgg gcaatggcga cattggtatg
    14881 gttatcgcca tcgtctttgt tgataacgat cagatctgcc acttccatca gccccttttt
    14941 aatgccctgc agatcatcgc cgccaccggc aatttgcaac gagataaaac agtccaccat
    15001 gcgggcgact tccgtttctg actgcccgac gccaaccgtt tcgacaatca ctacgtcata
    15061 acccgccgct tcgcataaca gcattaattc ccgcgctcgc tgactggcac cgcccagatg
    15121 gccggaggag ggtaccgggc gaataaacgc cgcctcggca cgcgcaaggt cattcatgcg
    15181 ggttttatcc ccgagaatgc taccgccagt gaccgggctg ctgggatcga ccgcaataac
    15241 cgcaaccttt aatccctcac gaatcaacaa catgccaaag gcttcaagaa aggtactttt
    15301 ccccgcgccg ggtgtgccgg taacgcccag tcgcagggcg ttaccacaga acggcataat
    15361 ggcgtcaagc agctgtgtac ttagcgcctg atgacgcggg tgacggcttt ccaccaacgt
    15421 catggcctgg gcgagcgtgg cacgctcacc ctgacgtaag cggcgaatac tttctgctag
    15481 cttggcttca ttaatcatga tgctggctta tcagattcag tacgtcgcgc acgctgtcga
    15541 gcataggtgt acctggacca taaatcgccg ccacgccgcg ctcttgcagg aaggcgtaat
    15601 cctgcggcgg aatgacgcca cccgcgacca cgcagatatc tttgcgtccc cattttttca
    15661 gcgcttcgac cagttccggg atcagcgttt tatgaccggc agccagtgag gatgcgccca
    15721 ctacgtgaac gtcgttttct acggccaggc gggcgatctc ttcaggtgta gagaacatcg
    15781 ggcttaaatc tacgtcgaaa ccgagatcgg aataggcgct ggcgatcact ttcgcgccgc
    15841 gatcgtgccc atcctggccc attttggcga tcagaatgcg cgggcgacga ccattgtcgg
    15901 caaggaactg ctccgtttgc gcaacaatgg catcgaactc ggcggccgat ttctcagact
    15961 gatgatagct ttgcgcaatc acgccggtaa cacactggct tggcaccaga taacggtcga
    16021 aagcggcttc cagcgcatcg gaaatttcac ccagggtggc gcgaacgcga gcagcattaa
    16081 cagcggcagc cagcaggttt tcgttatgct gtgcggcgtg agtcagggcg ttcaacgcgg
    16141 cggttacggc ggcatcatca cgggtggcgc gaatgcgttc cagcgaagca atttgctcgt
    16201 tacgcaccat cacgttgtcg atctcaagta catcggtttc gtcttcgtga tccagtttgt
    16261 acttgttgac accaacgatg acacgcttgc cctggtcgat cagcgactgt tcgcgcgctg
    16321 aggcctcttc gatcattcgt tttggcagac ctgcttcgat cgctttcgcc atgccaccgg
    16381 cttcgtcgat ctgttggata atagctctgg cttgtttgac gatttgatcg gtcagcgact
    16441 caatgtaata ggatccggcc agtggatcga cggtgcggca gagttctgat tcttcctgaa
    16501 tgatgatctg ggtattgcgg gcaatgcgtg ctgagaaatc ggtaggcaaa ccaagcgctt
    16561 catcaaaggc gttggtatgc agtgactgag taccgcccag cgttgcagcc agtgcttcaa
    16621 tggtggtgcg gataacgttg ttatacggat cctgttcagt caggctccag cctgaggtct
    16681 ggcagtgggt acgcagcgcc agtgatttcg gatcctgtgc gccaaatcca ctgaccgctt
    16741 cgctccataa ataacgtgcc gcacgcaaca tggcgacgtt cataaacaga tccatgccaa
    16801 tgccgaagaa gaacgacaga cgaggagcga agtcatcaat tttcagcccg gcggagattg
    16861 ctgctttgat gtactcaatc ccatcggcga gcgtaaatgc tacctgctgc acgcagttgg
    16921 cacccgcttc acccatgtgg taaccgctga tactgatggt attaaatcgc ggcatgttgc
    16981 cggaacacca ggcgatgatg tctgcgataa tacgcattga cggttttggc gggtaaatat
    17041 aggtgttgcg gcaaagatac tctttgagga tatcgttttg aatggtgccg gtcagtttat
    17101 cgggtgtaac accttgctct tctgcggcga caatataaaa cgccagtact ggtagtactg
    17161 cgccattcat ggtcatcgaa accgacattt tatccagcgg gatctggtcg aacaggactt
    17221 tcatatcttc cacggtgtcg atagcaacgc ccgctttgcc gacgtcgccc gccacgcgtg
    17281 ggttatcgga gtcgtagcct cggtgggtgg caaggtcaaa cgcaacggaa agaccttttt
    17341 gcccggcggc caggttacgg cgataaaaag cgttggactc ttttgctgtt gaaaaaccag
    17401 catactgacg gatggtccac ggttgggcgg tatacatagt ggcacgcggg ccacgaacgt
    17461 agggaggcaa accaggaagg gtacctgtca cctccagatt atcgagatcg gcttcggtat
    17521 acagcggctt gatggcgatc ccttccgcgg tttgctgaac cagcgagtcg acagttttct
    17581 cccgacggct caattccttg ttggcaagct gttgccactc ctgcacctta gacattattt
    17641 gctccgtaaa acgcaatcaa aatgagggta tttgcgctta cggtgagctt tttacgtggg
    17701 tttgtcgcca gcaaaaaaaa tgcattcaga cgtcgttttt tgccgcctga gatttgtcta
    17761 ttttgtgatg tgattcaagg aatacaaaaa gaagtgagga taacgcctga tatgtgctat
    17821 caggcattat ttgatggatt aatcctgacg aaggactttg tgaccataat cgagcaacgc
    17881 atcagtgact ttacgcatca tgcggctttc aggagcaaag cggtgccagt agagcatccg
    17941 tcgttgaaat agccctggcg ttaagtcaat cagttcaccg ctggccagct ctttctcgat
    18001 ttgcaggtgc gggatcatac agcaggtggt gccctgacga gcaagttgca cgaacgcttc
    18061 cgaagaatta acgatatggc agggtacgct gccaggaggc agatcgaagt tttgctgcaa
    18121 aaaggcctgg tgcatatcgt caagatggtc aaaagcgacc acaggtgctt tcaataatgc
    18181 cgaacgcgtt acgccgttag ggaaatattt ttcggcaaac ggttttgagc tgacgaacag
    18241 atagtcgagc gcaccgagct tatcgacaag gcaactcggc agcgcctgat gttgaatact
    18301 caccgcgccg accacttcgc cgcggcgcag acgttcctga gtgcgggttt catcttctac
    18361 ctgcaagttg aggcggatag gcgaatcagc taacacagga gccagcgccg gaagcaacca
    18421 cgtcgccaga ctgtcagcgt tgaccgccag tgaaagcagc agcggagttg aaccggtttg
    18481 ttcatcgccc agccactctt cttccagtaa ctccacctgg cgtagcagtg ccagcagttt
    18541 ttgcccttgt tctgtcgggc gcggtggtac ggtacgcacc aacagcggct gcccgaacat
    18601 attttccagt tgcttaatgc gctgtgagac ggctgattgt gtaatgcaca gcttttgtgc
    18661 cgcgcgctca aatcctcgtt cacgtatcac cgcatccagt gcctgtaatg ttctgtagtc
    18721 cgggcgtttc attgctgttg tctgctcctg gtaaatgctt tttcctgcac tatgacacaa
    18781 ttttgtgtca ggttgcatat accgaagtcg ttggcgggta acatttgtat cagtggttaa
    18841 cgcgcagctt aaaagatcag ggatttgcgc taatgcattt cgctcagcga gttcgcgccc
    18901 tggttgtgtt gaatggcgtg gcgttattgc ctctgttcgc atcgtggagc ggcataccca
    18961 gaccgttgta tgctttattt gcggggcgaa aggggatgcc cgccattgcg cgatatttta
    19021 tggatgagtt aaccacccgg cttgccaacg gggtctgaat cgcttttttt gtatataatg
    19081 cgtgtgaaat ttcataccac aggcgaaacg atcatgacgc aggatgaatt gaaaaaagca
    19141 gtaggatggg cggcacttca gtatgttcag cccggcacca ttgttggtgt aggtacaggt
    19201 tccaccgccg cacactttat tgacgcgctc ggcacgatga aaggccagat tgaaggggcc
    19261 gtttccagtt cagatgcttc cactgaaaaa ctgaaaagcc tcggcattca cgtttttgat
    19321 ctcaacgaag tcgacagcct tggcatctac gttgatggcg cagatgaaat caacggccac
    19381 atgcaaatga tcaaaggcgg cggcgcggcg ctgacccgtg aaaaaatcat tgcttcggtt
    19441 gcggaaaaat ttatctgtat tgccgacgcc tccaagcagg ttgatattct gggcaaattc
    19501 ccgctgccag tagaagttat cccgatggca cgtagtgcag tggcgcgcca gctggtgaaa
    19561 ctgggcggtc gtccggaata ccgtcagggc gtggtgaccg ataacggcaa cgtgatcctc
    19621 gacgtccacg gcatggaaat ccttgacccg atagcgatgg aaaacgccat aaatgcgatt
    19681 cctggcgtgg tgactgttgg cttgtttgct aaccgtggcg cggacgttgc gctgattggc
    19741 acacctgacg gtgtcaaaac cattgtgaaa tgatctgacg ggggaacctc ccccgttaaa
    19801 aaaattctct tcattaaatt tggtgacatg tgtcacgttt ttacctggct attgtcgatt
    19861 gctctaaata aaccctctaa accagcatat tcatccaaga aatacctttg cgtgatattt
    19921 cctcaatatc gtgacgcaaa cgttcatatt gccgcaatat tattttttga tatgttgaaa
    19981 ggcggatgca aatccgcaca caacatttca aaagacagga ttgggtaaat ggcaaaggta
    20041 tcgctggaga aagacaagat taagtttctg ctggtagaag gcgtgcacca aaaggcgctg
    20101 gaaagccttc gtgcagcggg ttacaccaac atcgaatttc acaaaggcgc gctggatgat
    20161 gaacaattaa aagaatccat ccgcgatgcc cacttcatcg gcctgcgatc ccgtacccat
    20221 ctggctgaag acgtgatcaa cgccgcagaa aaactggtcg ctattggctg tttctgtatc
    20281 ggaacaaacc aggttgatct ggatgcggcg gcaaaacgcg ggatcccggt atttaacgca
    20341 ccgttctcaa atacgcgctc tgttgcggag ctggtgattg gcgaactgct gctgctattg
    20401 cgcggcgtgc cggaagccaa tgctaaagcg catcgtggcg tgtggaacaa actggcggcg
    20461 ggttcttttg aagcgcgcgg caaaaagctg ggtatcatcg gctacggtca tattggtacg
    20521 caattgggca ttctggctga atcgctggga atgtatgttt acttttatga tattgaaaac
    20581 aaactgccgc tgggcaacgc cactcaggta cagcatcttt ctgacctgct gaatatgagc
    20641 gatgtggtga gtctgcatgt accagagaat ccgtccacca aaaatatgat gggcgcgaaa
    20701 gagatttcgc taatgaaacc cggctcgctg ctgattaatg cttcgcgcgg tactgtggtg
    20761 gatattccag cgctgtgtga cgcgctggcg agcaaacatc tggcaggggc ggcaatcgac
    20821 gtattcccga cggaaccggc gaccaatagc gatccattta cctctccgct gtgtgaattc
    20881 gacaatgtcc ttctgacgcc acacattggt ggttcgactc aggaagcgca ggagaatatc
    20941 ggtctggaag ttgcgggtaa attgatcaag tattctgata atggttcaac gctctctgcg
    21001 gtgaacttcc cggaagtctc gctgccactg cacggtggtc gtcgtctgat gcacatccac
    21061 gaaaaccgtc cgggtgtgct gactgcgctc aacaaaattt ttgccgagca gggcgtcaac
    21121 atcgccgcgc aatatctgca aacttccgcc cagatgggtt atgtagttat tgatattgaa
    21181 gccgacgaag acgttgccga aaaagcgctg caggcaatga aagctattcc gggtaccatt
    21241 cgcgcccgtc tgctgtacta aatcccctta tctgaaaatc aacgggcagg tcactgactt
    21301 gcccgttttt ttatcccttc tccacaccgt tgccgcattg cgcattctac tttgcgagtc
    21361 tgctccagaa aataaatcaa agtcagatca atgcgttgtg tttatttaat ggggatttac
    21421 gatggcaggg cagcatgggg ctgtaacggt aaagccctca ccgaagcgag ggcttgaagg
    21481 agaagggtta tgatgcgact tgtcatcata ctgattgtac tgttactcat aagtttccca
    21541 gcttattaac agtcagtctc aggggaggag tcatcctccc ttacccttac tcactaaatt
    21601 aggttaaaga atcaacgatg tcaatcaggg cgatgcaggt gtgtcgccct caccactccc
    21661 agactttcga cggtgtaacc accgcaggaa gagggatatc ccactcttca acggggagtt
    21721 tttccaccaa ctgacaatca tgcgcataac ccaccggttg cattttatag tgctgccagt
    21781 tttgtaaggt ccgatcataa aaaccaccgc ccattcccag gcgctgaccg tactcatcaa
    21841 aggcgaccag cggtgtgatc aacacgtcta atcgggaaag gggtagcacg tcacgcacat
    21901 ccagtttggg ctcatggatc ttcaacctgt tcatcaccag ttcgctttgc ggatggtaat
    21961 tcaggaacag caaattaccg gcactgaagg gatgcaaaac tggaagatat acgcgcttac
    22021 cggcgcgcca gagttgttct atgagtggca gggtgtcgag ttcgccatca aaagagagga
    22081 atacagcgac cgtatgtgcc atcaccaccg ggggataagt catcatccgg gtagcggctt
    22141 gttgacccat ttcctgctgt tgttccggcg ttaacgcacg acgacgttgc cgaatcattt
    22201 tgcggatttc ttgtcgggat aatgtcagtg ggagttctgg tagttgcgtc atggctggtg
    22261 ccagataaga agggaatctc cgagatgccg ccgcaggctg taacccttga acccttggtt
    22321 caaggtgaat gtgtcgtcgc agttttaagg cttctcggac ggaccgagca tgctcaccaa
    22381 ccgcggagcg ccacattctt gtggtatgaa atatcggctc aggggactgg cccgcttgcg
    22441 aacatctcag agaaattttg tcttcacggt tactctacca cagtaaaccg aaaagtgtca
    22501 ttcaaagttt tggttagttt tttcggtgat gcgaccttgt tcaaataacg cttgttctat
    22561 ggtctgctgc agcatccgaa tacgctgttc catacttgcc gcatagtcac gagtcttcgc
    22621 tttttcttgc gctaactcat agctgatatt caatgcggca atgaagacca actgttcagt
    22681 atttgtgact ctagtgcgtt ctttcagatc ttgcaaccgt tggttcagat cgtccgctgc
    22741 ctgattcaac gcatcccttt ggtcaggcgg gcagttcaca cgcagtgaac ggccaaaaat
    22801 ttggatatcg acgggttgtg cagacatgcc accttcctgc tgattgactg cgctgccttc
    22861 gtctttagac ccatggtctg cgaaggggcg acactatagc taccctgatg agaagagaca
    22921 agcccttttc tggtccacca gggcccaaag tggtagcata tcatgaatat tcctcccttt
    22981 gacgacgaat gcttatgtct atacagaacg aaatgcctgg ttacaacgaa atgaaccagt
    23041 atctgaacca acaagggacg ggtctgaccc ctgctgagat gcatggttta atcagcggga
    23101 tgatatgtgg cggtaacgat gacagctcat ggctgccgct acttcacgac ctgacgaacg
    23161 aaggcatggc tttcggtcat gagctggcac aggcactgcg taaaatgcac tctgccacca
    23221 gcgatgccct gcaggatgac ggcttccttt ttcagcttta tctgcctgat ggcgatgatg
    23281 tcagcgtttt cgatcgggct gatgcgctgg caggttgggt caatcacttc ctgcttggtc
    23341 ttggcgttac gcaaccgaag ctggacaaag tgaccggaga aaccggtgaa gccatcgacg
    23401 atctgcgtaa catcgcgcag ctgggctacg acgaagacga agatcaggaa gagcttgaaa
    23461 tgtcgcttga agagatcatc gaatacgttc gtgttgccgc gctgttatgc cacgacacct
    23521 ttactcatcc gcaaccgacc gcgccagaag tacaaaaacc gactctacac taaaaacaaa
    23581 aaacgtaagg agagtgttat gagtgagata tcccggcaag agtttcagcg tcgccgtcag
    23641 gccctggtgg agcaaatgca acccggcagc gccgcgctga tttttgctgc accagaagta
    23701 acacgtagcg ccgacagcga atacccctat cgtcagaaca gtgacttctg gtacttcacc
    23761 ggctttaacg aaccggaagc ggtgctggtg ctgattaaaa gcgacgacac tcataaccac
    23821 agcgttttgt ttaaccgcgt tcgcgacctg acggcagaga tctggtttgg tcgtcgctta
    23881 ggccaggatg ccgcgccaga gaaactgggc gttgaccgcg cactggcatt cagcgaaatc
    23941 aatcagcaac tttatcaact gcttaatggt ctggatgtgg tttatcacgc tcagggcgaa
    24001 tatacctatg ctgatgaaat cgtgaacagc gcgctggaaa agctgcgtaa aggctcgcga
    24061 cagaatctca ctgccccggc tacgatgatc gactggcgtc ctgttgttca tgaaatgcgc
    24121 ctgttcaaat caccagaaga gattgccgta cttcgccgcg cgggtgaaat caccgcactg
    24181 gcgcataccc gggcgatgga aaaatgccgt ccggggatgt tcgagtacca tctggaaggc
    24241 gaaattcacc acgagtttaa ccgccacggt gcgcgttacc cttcctataa caccattgtt
    24301 ggcagcggtg aaaacggctg cattttgcac tacaccgaaa acgagtgtga actgcgtgac
    24361 ggtgatctgg tgttgattga cgcgggttgt gaatacaaag gttacgctgg cgacattacc
    24421 cgcaccttcc cggtcaacgg caaattcact caggctcagc gtgaaatcta tgacattgtg
    24481 ctggagtctc ttgaaaccag cctgcgcctg tatcgtccgg gaacttccat gcaggaagtc
    24541 actggtgaag tggtgcgtat catggttagc ggcctggtaa aactcggcat cctgaagggt
    24601 gatgttgatg aactgatcgc tcagaacgcc catcgtcctt tctttatgca tggccttagc
    24661 cactggttag gactggatgt ccatgacgtt ggcgtttatg gtcaggatcg ctcgcgcatt
    24721 ctggaaccgg ggatggtact gaccgtagag cctggtctgt atatcgcgcc ggatgcggat
    24781 gtgccagaac aatatcgcgg tatcggcatt cgtattgaag acgacattgt gattaccgaa
    24841 accggcaacg aaaacctcac cgccagcgtg gtgaaaaagc cggaagaaat cgaagcgttg
    24901 atggctgctg cgagaaagca atgagcgtaa tcatcgtcgg tggcggcatg gcgggcgcga
    24961 cgctggcgct ggctatttcc cggttaagtc acggggcgct gccggtacat ttgattgaag
    25021 cgactgcgcc agagtcacat gctcatccgg gctttgatgg acgagcgata gcgctggcgg
    25081 cgggtacctg tcagcaactg gcgcgcatcg gcgtctggca atctctggcg gattgcgcaa
    25141 ccgccatcac taccgtgcat gtcagcgatc gtggtcatgc cggattcgtc accctcgccg
    25201 cagaagatta ccaactggcg gcgctggggc aggttgtcga attgcataat gtcgggcaac
    25261 ggctgtttgc attgctgcgc aaagcacctg gcgtaacgct gcattgtcct gatcgcgtgg
    25321 ctaacgttgc ccgtactcag agtcacgttg aagtgacgct ggagagtggc gagacgctga
    25381 cgggctgcgt gctggtagcc gctgatggca cccattcagc gttagccacc gtgtgcggcg
    25441 ttgactggca gcaggagcct tacgaacaac tggctgtgat tgccaacgtt gcgacttccg
    25501 ttgcgcatga agggcgcgct tttgaacgct ttacacaata tggcccgctg gcgatgttgc
    25561 cgatgtctga cggacgctgt tcgctggtct ggtgtcatcc actggaacgg cgcgaagagg
    25621 tactgtcttg gagcgacgag aagttttgcc gtgaactcca gtcggccttt ggttggcgac
    25681 ttgggaaaat tacccacgct ggtaaacgca gtgcttatcc gctggcgtta acccgcgccg
    25741 ccagacctat tacccatcgt accgtgctgg tgggcaatgc ggcgcaaact ctgcacccga
    25801 ttgccgggca agggttcaac ctcggtatgc gagatgtcat gagccttgcg gaaaccctga
    25861 ctcaggcgca ggagcgcgga gaagacatgg gtgattacgg cgtattgtgc cgttatcagc
    25921 agcgtcgaca gcgcgatcgc gaagcaacca tcggcgtcac ggacagcctt gtacatcttt
    25981 ttgccaaccg ttgggggccg ctggttgtcg ggcgcaacat cgggctgatg gcaatggaat
    26041 tattcacccc agcacgcgat gtgctggcgc agcgcaccct cgggtgggtg gcgcgttgaa
    26101 ggcggtttta aggaataaac aatgcaaagt gttgatgtag ccattgttgg cggcggcatg
    26161 gtggggctgg cggttgcctg cggcttgcag ggtagcggct tacgcgttgc cgtactggag
    26221 cagcgcgtac cggaacctct ggcggcggat gcaccaccac aactgcgcgt ttcggctatc
    26281 aatgccgcca gcgaaaaatt actcacccgt cttggtgtct ggcaggacat tctctctcgt
    26341 agggccagtt gttatcacgg tatggaagtg tgggacaaag acagttttgg tcacatttcg
    26401 tttgacgatc aaagcatggg ctatagccat cttgggcata tcgttgaaaa ctcggtgatt
    26461 cactacgcgc tgtggaacaa agcgcagcag tcgtcagata tcactctgtt ggcccccgca
    26521 gaattacagc aggtcgcctg gggagaaaat gaaaccttcc tgacgttgaa agatggcagt
    26581 atgttaacgg cgcgtctggt gattggcgcg gacggcgcta attcctggtt gcgcaacaaa
    26641 gccgatattc cgctgacttt ctgggattat cagcatcacg cgctggtagc gaccattcgc
    26701 acggaagaac cgcatgatgc ggtggcgcgg caggtttttc atggcgaagg cattctggcc
    26761 tttttgccgc ttagcgatcc gcatctttgc tcgattgtct ggtcactgtc gccagaggaa
    26821 gcgcagcgga tgcagcaggc aggcgaagac gaatttaatc gcgcgttaaa tatcgctttt
    26881 gataatcgcc tgggcttatg caaggttgaa agtgagcgcc tggtgttccc gctaacgggg
    26941 cgttatgcgc gccagtttgc cgcacaccgt ctggcgctgg tgggcgacgc cgcgcatact
    27001 attcacccgc tggcggggca gggggtaaat ctcggcttta tggatgctgc agagctgatt
    27061 gctgaactga aacggttgca tcgtcagggt aaagacatcg ggcagtacat ttacctgcgt
    27121 cgctatgagc gtagccgtaa gcacagtgcg gcgctgatgc tggctggtat gcagggattc
    27181 cgcgatctgt tttccggtac caatccggcg aaaaaattgc tgcgtgatat tggtctgaaa
    27241 ctggctgata cgcttcctgg cgttaagcca caacttattc gtcaggcaat gggattaaac
    27301 gatttgcctg aatggctgcg ttaaatattt ctcttctgct gtttatttga tacccatcac
    27361 actttcatct cccggttttt tcgccgggag attttcctca tttgaaataa
//
LOCUS       DAZFLD010000048        27303 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.048, whole genome shotgun sequence.
ACCESSION   DAZFLD010000048 DAZFLD010000000
VERSION     DAZFLD010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27303
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.048"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            96..755
                     /gene="yqfA"
                     /locus_tag="ACHKBL_001682"
     CDS             96..755
                     /gene="yqfA"
                     /locus_tag="ACHKBL_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HFO5818562.1"
                     /translation="MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDL
                     NASATAITSYSLYGGSMILLFLASTLYHAIPHQRAKMWLKKFDHCAIYLLIAGTYTPF
                     LLVGLDSPLARGLMIVIWSLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMA
                     IKLAAGSVTLLAVGGVVYSLGVIFYVCKRIPYNHAIWHGFVLGGSVCHFLAIYLYIGQ
                     A"
     gene            complement(832..1812)
                     /gene="ygfZ"
                     /locus_tag="ACHKBL_001683"
     CDS             complement(832..1812)
                     /gene="ygfZ"
                     /locus_tag="ACHKBL_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-modifying protein YgfZ"
                     /protein_id="HFO5818563.1"
                     /translation="MAFTPFPPRQPTASARLPLTLMTLDDWALATITGADSEKYMQGQ
                     VTADVSQMTEDQHLLAAHCDAKGKMWSNLRLFRDGDGFAWIERRSVREPQLTELKKYA
                     VFSKVTIAPDDERVLLGVAGFQARAALANIFSELPSKEKQVVKEGATTLLWFEHPAER
                     FLIVTDEATANMLTDKLRGEAELNNSQQWLALNIEAGFPVIDAANSGQFIPQATNLQA
                     LGGISFKKGCYTGQEMVARAKFRGANKRALWLLTGSASRLPEAGEDLELKMGENWRRT
                     GTVLAAVKLEDGQVVVQVVMNNDMEPDSIFRVRDDANTLHIEPLPYSLEE"
     gene            2065..2331
                     /gene="sdhE"
                     /locus_tag="ACHKBL_001684"
     CDS             2065..2331
                     /gene="sdhE"
                     /locus_tag="ACHKBL_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HFO5818564.1"
                     /translation="MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFI
                     RLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRNRERGPVAI"
     gene            2312..2719
                     /gene="ygfX"
                     /locus_tag="ACHKBL_001685"
     CDS             2312..2719
                     /gene="ygfX"
                     /locus_tag="ACHKBL_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgfX"
                     /protein_id="HFO5818565.1"
                     /translation="MVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMV
                     LLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQEWSIVKAPWMIKSGMMLRLRSD
                     SGKRQHLWLAADSMDEAEWRDLRRILLQQETQR"
     gene            complement(2759..3280)
                     /gene="fldB"
                     /locus_tag="ACHKBL_001686"
     CDS             complement(2759..3280)
                     /gene="fldB"
                     /locus_tag="ACHKBL_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldB"
                     /protein_id="HFO5818566.1"
                     /translation="MNMGLFYGSSTCYTEMAAEKIRDIIGPELVTLHNLKDDSPKLME
                     QYDVLILGIPTWDFGEIQEDWEAVWDQLDDLNLEGKIVALYGLGDQLGYGEWFLDALG
                     MLHDKLSTKGVKFVGYWPTEGYEFTSPKPVIADGQLFVGLALDETNQYDLSDERIQSW
                     CEQILNEMAEHYA"
     gene            3392..4288
                     /gene="xerD"
                     /locus_tag="ACHKBL_001687"
     CDS             3392..4288
                     /gene="xerD"
                     /locus_tag="ACHKBL_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HFO5818567.1"
                     /translation="MKQDLARIEQFLDALWLEKNLAENTLNAYRRDLSMMVEWLHHRG
                     LTLATAQSDDLQALLAERLEGGYKATSSARLLSAVRRLFQYLYREKFREDDPSAHLAS
                     PKLPQRLPKDLSEAQVERLLQAPLIDQPLELRDKAMLEVLYATGLRVSELVGLTMSDI
                     SLRQGVVRVIGKGNKERLVPLGEEAVYWLETYLEHGRPWLLNGVSIDVLFPSQRAQQM
                     TRQTFWHRIKHYAVLAGIDSEKLSPHVLRHAFATHLLNHGADLRVVQMLLGHSDLSTT
                     QIYTHVATERLRQLHQQHHPRA"
     gene            4313..5023
                     /gene="dsbC"
                     /locus_tag="ACHKBL_001688"
     CDS             4313..5023
                     /gene="dsbC"
                     /locus_tag="ACHKBL_001688"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HFO5818568.1"
                     /translation="MKKGFMLFTLLAAFSGFVQADDAAIQQTLAKMGIKSSDIQPAPV
                     AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGTAPVNVTNKMLLKQLNALEKEMIVYK
                     APQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLDSDAEKEMKAIW
                     CAKDKNKAFDDVMAGKSVAPASCDVDIADHYALGVQLGVSGTPAVVLSNGTLVPGYQP
                     PKDMKEFLDEHQKMTSGK"
     gene            5029..6762
                     /gene="recJ"
                     /locus_tag="ACHKBL_001689"
     CDS             5029..6762
                     /gene="recJ"
                     /locus_tag="ACHKBL_001689"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HFO5818569.1"
                     /translation="MKQQIQLRRREVDETADLPAELPPLLRRLYASRGVRSAQELERS
                     VKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDFDADGATSTALSVLAMRSLGCSN
                     IDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHSGVEHARSLGIPVIVTD
                     HHLPGETLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERGVA
                     IPNLAELLDLVALGTVADVVPLDANNRILTWQGMSRIRAGKCRPGIKALLEVANRDAL
                     KLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLANELDALNQTRKEIEQ
                     GMQIEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAG
                     DGTLKGSGRSIQGLHMRDALERLDTLYPGMMLKFGGHAMAAGLSLEEDKFELFQQRFG
                     ELVTEWLDPSLLQGEVVSDGPLSPAEMTMEVAQLLRDAGPWGQMFPEPLFDGHFRLLQ
                     QRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLTYKLDINEFRGNRS
                     LQIIIDNIWPI"
     gene            6853..7951
                     /gene="prfB"
                     /locus_tag="ACHKBL_001690"
     CDS             join(6853..6927,6929..7951)
                     /gene="prfB"
                     /locus_tag="ACHKBL_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311790.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HFO5818570.1"
                     /translation="MFEINPVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQP
                     DVWNEPERAQALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEADDEETFNEAVAE
                     LDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRG
                     FKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFS
                     SAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNRTESAVRITHIPTGIVTQCQN
                     DRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DLRTGVETRNTQAVLDGSLDQFIEASLKAGL"
     gene            7961..9478
                     /gene="lysS"
                     /locus_tag="ACHKBL_001691"
     CDS             7961..9478
                     /gene="lysS"
                     /locus_tag="ACHKBL_001691"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFO5818571.1"
                     /translation="MSEQHAQGADAVVDLNNELKTRREKLANLREQGIAFPNDFRRDH
                     TSDQLHAEFDGKENEELEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     DLPEGVYNEQFKKWDLGDILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEARYRQRYLDLISNDESRNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGA
                     AARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYKDLIELTESLFRTLAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDSAKAIAESIGIHVEKSWGLGRIVTEIFEEVAEAHLIQPTFITE
                     YPAEVSPLARRNDVNPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFLDQVAAKDAG
                     DDEAMFYDEDYVTALEHGLPPTAGLGIGIDRMVMLFTNSHTIRDVILFPAMRPVK"
     gene            complement(9521..10069)
                     /gene="idi"
                     /locus_tag="ACHKBL_001692"
     CDS             complement(9521..10069)
                     /gene="idi"
                     /locus_tag="ACHKBL_001692"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HFO5818572.1"
                     /translation="MQTEHVILLNAQGVPTGTLEKYAAHTADTLLHLAFSSWLFNAKG
                     QLLVTRRALSKKAWPGVWTNSVCGHPQLGESNEDAVIRRCRYELGVEITPPESIYPDF
                     YYRATDPNGIVENEVCPVFAARTTSALQINDDEVMDYQWCDLADVLHGIDATPWAFSP
                     WMVMQAANSEARKLLSAFAQHN"
     gene            10124..10195
                     /locus_tag="ACHKBL_001693"
     CDS             10124..10195
                     /locus_tag="ACHKBL_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5818573.1"
                     /translation="MINQVSVYRQPPVLSGCRQVKTI"
     gene            complement(10192..10317)
                     /gene="yqfG"
                     /locus_tag="ACHKBL_001694"
     CDS             complement(10192..10317)
                     /gene="yqfG"
                     /locus_tag="ACHKBL_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqfG"
                     /protein_id="HFO5818574.1"
                     /translation="MNLLMRAIFSLLLLFTLSIPVISDCVAMAIESRFKYMMLLF"
     gene            complement(10319..11767)
                     /gene="uacT"
                     /locus_tag="ACHKBL_001695"
     CDS             complement(10319..11767)
                     /gene="uacT"
                     /locus_tag="ACHKBL_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate/proton symporter UacT"
                     /protein_id="HFO5818575.1"
                     /translation="MSAIDSQLPSSSGQDRPTDEVDRILSPGKLIILGLQHVLVMYAG
                     AVAVPLMIGDRLGLSKEAIAMLISSDLFCCGIVTLLQCIGIGRFMGIRLPVIMSVTFA
                     AVTPMIAIGMNPDIGLLGIFGATIAAGFITTLLAPLIGRLMPLFPPLVTGVVITSIGL
                     SIIQVGIDWAAGGKGNPQYGNPVYLGISFAVLIFILLITRYAKGFMSNVAVLLGIVFG
                     FLLSWMMNEVNLSGLHDASWFAIVTPMSFGMPIFDPVSILTMTAVLIIVFIESMGMFL
                     ALGEIVGRKLSSHDIIRGLRVDGVGTMIGGTFNSFPHTSFSQNVGLVSVTRVHSRWVC
                     ISSGIILILFGMVPKMAVLVASIPQFVLGGAGLVMFGMVLATGIRILSRCNYTTNRYN
                     LYIVAISLGVGMTPTLSHDFFSKLPAVLQPLLHSGIMLATLSAVVLNVFFNGYQHHAD
                     LVKESVSDKDLKVRTVRMWLLMRKLKKNEHGE"
     gene            12202..14121
                     /gene="ygfT"
                     /locus_tag="ACHKBL_001696"
     CDS             12202..14121
                     /gene="ygfT"
                     /locus_tag="ACHKBL_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     YgfT"
                     /protein_id="HFO5818576.1"
                     /translation="MNKFIAAEAAECIGCHACEIACAMAHNQENWPLSHSDFRPRIHV
                     VGKGQAANPVACHHCNNAPCVTACPVNALTFQPDSVQLDEQKCIGCKRCAIACPFGVV
                     EMVDTIAQKCDLCNQRSSGTQACIEVCPTQALRLMDDKGLQQIKVARQRKTAAGKASS
                     DAQPSRSAALLPVNSRKGADKISASERKTHFGEIYYGLDPQQATYESDRCVYCAEKAN
                     CNWHCPLHNAIPDYIRLVQEGKIIEAAELCHQTSSLPEICGRVCPQDRLCEGACTLKD
                     HSGAVSIGNLERYITDTALAMGWRPDVSKVVPRIEKVAVIGAGPAGLGCADILARAGV
                     QVDVFDRHPEIGGMLTFGIPPFKLDKTVLSQRREIFTAMGIDFHLNCEIGRDITFSDL
                     TSEYDAVFIGVGTYGMMRADLPHEDAPGVIQALPFLTAHTRQLMGLPESEEYPLTDVE
                     GKRVVVLGGGDTTMDCLRTSIRLNAASVTCAYRRDEVSMPGSRKEVVNAREEGVEFQF
                     NVQPQYIACDEDGRLTAVGLIRTAMGEPGPDGRRRPRPVAGSEFELPADVLIMAFGFQ
                     AHAMPWLQGSGIKLDKWGLIQTGDVGYLPTQTHLKKVFAGGDAVHGADLVVTAMAAGR
                     QAARDMLTLFDTKAS"
     gene            14121..14609
                     /gene="ygfS"
                     /locus_tag="ACHKBL_001697"
     CDS             14121..14609
                     /gene="ygfS"
                     /locus_tag="ACHKBL_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFO5818577.1"
                     /translation="MKSLIIVNPADCIGCRTCEVACVVAHPSEQELNADVFLPRLKVQ
                     RLDSISAPVMCHQCENAPCVSACPVGALTMGEQVVQTNSARCIGCQSCVSACPFGMIT
                     IQSLPGDTRQQIVKCDLCEQREEGPACVESCPTQALQLLTERELRRVRQQRIVASGEN
                     PL"
     gene            complement(14645..16012)
                     /gene="ghxQ"
                     /locus_tag="ACHKBL_001698"
                     /gene_synonym="ygfQ"
     CDS             complement(14645..16012)
                     /gene="ghxQ"
                     /locus_tag="ACHKBL_001698"
                     /gene_synonym="ygfQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxQ"
                     /protein_id="HFO5818578.1"
                     /translation="MSGDILQTPDAPKPQGALDNYFKITARGSTVRQEVLAGLTTFLA
                     MVYSVIVVPGMLGKAGFPPAAVFVATCLVAGFGSLLMGLWANLPMAIGCAISLTAFTA
                     FSLVLGQQISVPVALGAVFLMGVIFTAISVTGVRTWILRNLPMGIAHGTGIGIGLFLL
                     LIAANGVGMVIKNPIEGLPVALGAFTSFPVMMSLLGLAVIFGLEKCRVPGGILLVIIA
                     ISIIGLIFDPAVKYHGLVAMPSLTGEDGKSLIFSLDIMGALQPTVLPSVLALVMTAVF
                     DATGTIRAVAGQANLLDKDNQIINGGKALTSDSVSSIFSGLVGAAPAAVYIESAAGTA
                     AGGKTGLTATVVGALFLLILFLSPLSFLIPGYATAPALMYVGLLMLSNVSKLDFNDFI
                     DAMAGLVCAVFIVLTCNIVTGIMLGFVTLVVGRVFAREWQKLNIGTVIITAALVAFYA
                     GGWAI"
     gene            complement(16048..17364)
                     /gene="guaD"
                     /locus_tag="ACHKBL_001699"
     CDS             complement(16048..17364)
                     /gene="guaD"
                     /locus_tag="ACHKBL_001699"
                     /EC_number="3.5.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008892 - guanine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006147 - guanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanine deaminase"
                     /protein_id="HFO5818579.1"
                     /translation="MSGEHTLKAVRGSFIDVTRTVDNPEEIASALRFIEDGLLLIKQG
                     KVEWFGEWEDGKHQIPDTIRVRDYRGKLIVPGFVDTHIHYPQSEMVGAYGEQLLEWLN
                     KHTFPTERRYEDLEYAREMSAFFIKQLLRNGTTTALVFGTVHPQSVDALFEAASHINM
                     RMIAGKVMMDRNAPDYLLDTAESSYHQSKELIERWHKNGRLLYAITPRFAPTSSPEQM
                     AMAQRLKEEYPDTWVHTHLCENKDEIAWVKSLYPDHDGYLDVYHQYGLTGKNCVFAHC
                     VHLEEKEWDRLSETKSSIAFCPTSNLYLGSGLFNLKKAWQKKVKVGMGTDIGAGTTFN
                     MLQTLNEAYKVLQLQGYRLSAYEAFYLATLGGAKSLGLDDLIGNFLPGKEADFVVMEP
                     TATPLQQLRYDNSVSLVDKLFVMMTLGDDRSIYRTYVDGRLVYERN"
     gene            complement(17382..18782)
                     /gene="xanQ"
                     /locus_tag="ACHKBL_001700"
     CDS             complement(17382..18782)
                     /gene="xanQ"
                     /locus_tag="ACHKBL_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanQ"
                     /protein_id="HFO5818580.1"
                     /translation="MSDINHAGSDLIFELEDRPPFHQALVGAITHLLAIFVPMVTPAL
                     IVGATLQLSAETTAYLVSMAMIASGIGTWLQVNRYGIVGSGLLSIQSVNFSFVTVMIA
                     LGSSMKSDGFHEELIMSSLLGVSFVGAFLVVGSSFILPYLRRVITPTVSGIVVLMIGL
                     SLIKVGIIDFGGGFAAKSSGTFGNYEHLGVGLLVLIVVIGFNCCRSPLLRMGGIAIGL
                     CVGYIASLCLGMVDFSSMRNLPLITIPHPFKYGFSFSFHQFLVVGTIYLLSVLEAVGD
                     ITATAMVSRRPIQGEEYQSRLKGGVLADGLVSVIASAVGSLPLTTFAQNNGVIQMTGV
                     ASRYVGRTIAVMLVILGLFPMIGGFFTTIPSAVLGGAMTLMFSMIAIAGIRIIITNGL
                     KRRETLIVATSLGLGLGVSYDPEIFKILPASIYVLVENPICAGGLTAILLNIILPGGY
                     RQENVLPGITSAEEMD"
     gene            complement(18947..21817)
                     /gene="xdhD"
                     /locus_tag="ACHKBL_001701"
     CDS             complement(18947..21817)
                     /gene="xdhD"
                     /locus_tag="ACHKBL_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     Mo/Fe-S-binding subunit"
                     /protein_id="HFO5818581.1"
                     /translation="MIIHFTLNGTPQELTVNPGENVQKLLFNMGMHSVRNSDDGFGFA
                     GSDAIIFNGNIVNASLLIAAQLEKADIRTAESLGKWNELSLVQQAMVDVGVVQSGYND
                     PAAALIITDLLDRIDAPTREEIDDALSGLFSRDAGWQQYYQVIELAVARKNNPQATID
                     IAPTFRDDLEVIGKHYPKTDAAKMVQAKPCYVEDRVTADACVIKMLRSPHAHALITHL
                     DVSKAEALPGVVHVITHLNCPDIYYTPGGQSAPEPSPLDRRMFGKKMRHVGDRVAAVV
                     AESEDIALEALKLIDVEYEVLKPVMSIDEAMAEDAPVVHDEPVVYVAGAPDTLEEDNC
                     HAAQRGEHMIINFPIGSRPRKNIAASIHGHIGDMDKGFADADVIIERTYNSTQAQQCP
                     TETHICFTRMDGDRLVIHASTQVPWHLRRQVARLVGMKQHKVHVIKERVGGGFGSKQD
                     ILLEEVCAWATCVTGRPVLFRYTREEEFIANTSRHVAKVTVKLGAKRDGRLTAVKMDF
                     RANTGPYGNHSLTVPCNGPALSLPLYPCDNVDFQVTTYYSNICPNGAYQGYGAPKGNF
                     AITMALAELAEQLQIDQLEIIERNRVHEGQELKILGAIGEGKAPTSVPSAASCALEEI
                     LRQGREMIQWSSPKPQNGDWHIGRGVAIIMQKSGIPDIDQANCMIKLESDGTFIVHSG
                     GADIGTGLDTVVTKLAAEVLHCPPQDVHVISGDTDHALFDKGAYASSGTCFSGNAARL
                     AAENLREKILFHGAQMLGEPVADVQLATPGVVRGKKGEVSFGEIAHKGETGTGFGSLV
                     GTGSYITPDFAFPYGANFAEVAVNTRTGEIRLDKFYALLDCGTPVNPELALGQIYGAT
                     LRAIGHSMSEEIIYDAEGHPLTRDLRSYGAPKIGDIPRDFRAVLVPSDDKVGPFGAKS
                     ISEIGVNGAAPAIATAIHDACGIWLREWHFTPEKILTALEKI"
     gene            complement(21814..22593)
                     /gene="ygfM"
                     /locus_tag="ACHKBL_001702"
     CDS             complement(21814..22593)
                     /gene="ygfM"
                     /locus_tag="ACHKBL_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     FAD-binding subunit"
                     /protein_id="HFO5818582.1"
                     /translation="MIEQFFRPDSVEQALELKRRYQDEAVWFAGGSKLNATPTRTDKK
                     IAISLQDLELDWVDWDNGALRIGAMSRLQTLRDARFIPAALREALGFVYSRHVRNQST
                     IGGEIAARQEESVLLPVLLALDAELVFGNGETLSIEEYLACPCDRLLTEIIIKDPYRT
                     CATRKISRSQAGLTVVTAAVAITDHDGMRIALDGVASKALRLHDVEKQNLEGDALEQA
                     VANAIFPQEDLRGSVAYKRYITGVLVADLYADCQQAGEEAV"
     gene            complement(22644..23972)
                     /gene="ssnA"
                     /locus_tag="ACHKBL_001703"
     CDS             complement(22644..23972)
                     /gene="ssnA"
                     /locus_tag="ACHKBL_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminohydrolase SsnA"
                     /protein_id="HFO5818583.1"
                     /translation="MLILKNVTAVQLHPAKVQEGVDIAIENDVIVAIGDALTQRYPDA
                     SYKEMHGRIVMPGIVCSHNHFYSGLSRGIMANIAPCPDFISTLKNLWWRLDRALDEES
                     LYYSGLICSLEAIKGGCTSVIDHHASPAYIDGSLSTLRNAFLKVGLRAMTCFETTDRN
                     NGIKELQEGVEENIRFARQIDDAKKAATEPYLVEAHIGAHAPFTVPDAGLEMLREAVK
                     ATGRGLHIHAAEDLYDVSHSHHWYGKDLLARLAQFDLIDSKTLVAHGLYLSKDDIALL
                     NQRDAFLVHNARSNMNNHVGYNHHLCDIRNLALGTDGIGSDMFEEMKFAFFKHRDAGG
                     PLWPDSFAKALANGNELMSRNFGAKFGLLEAGYKADLTICDYNSPTPLLADNIAGHIA
                     FGMGSGSVHSVMVNGVMVYEDRQFNFDCDSIYAQARKAAASMWRRMDALA"
     gene            complement(23975..27073)
                     /gene="ygfK"
                     /locus_tag="ACHKBL_001704"
     CDS             complement(23975..27073)
                     /gene="ygfK"
                     /locus_tag="ACHKBL_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenate reductase subunit YgfK"
                     /protein_id="HFO5818584.1"
                     /translation="MGDIMRPIPFEELLTRIFDEYQQQRSIFGIPEQQFYSPVKGKTV
                     SVFGETCATPVGPAAGPHTQLAQNIVTSWLTGGRFIELKTVQILDRLELEKPCIDAED
                     ECFNTEWSTEFTLLKAWDEYLKAWFALHLLEAMFQPSDSCKSFIFNMSVGYNLEGIKQ
                     PPMQQFIDNMMDASDHPKFAQYRDALNKLLQDDAFLARHGLQEKRESLQALPARIPTS
                     MVQGVTLSTMHGCPPHEIEAICRYMLEEKGLNTFVKLNPTLLGYARVREILDVCGFGY
                     IGLKEESFDHDLKLTQALEMLERLMALAKEKSLGFGVKLTNTLGTINNKGALPGEEMY
                     MSGRALFPLSINVAAVLSRAFDGKLPISYSGGASQLTIRDIFDTGIRPITMATDLLKP
                     GGYLRLSACMRELEGSDAWGLDHVDVERLNRLAADALTMEYTQKHWKPEERIEVAEDL
                     PLTDCYVAPCVTACAIKQDIPEYIRLLGEHRYADALELIYQRNALPAITGHICDHQCQ
                     YNCTRLDYDSALNIRELKKVALEKGWDEYKQRWHKPAGSGSRHPVAVIGAGPAGLAAG
                     YFLARAGHPVTLFEREANAGGVVKNIIPQFRIPPELIQHDIDFVAAHGVKFEYGCSPD
                     LTVEQLKNQGFHYVLIATGTDKNSGVKLAGDNQNVWKSLPFLREYNKGTELKLGKHVV
                     VVGAGNTAMDCARAALRVPGVEKATVVYRRSLQEMPAWREEYEEALHDGVEFRFLNNP
                     ERFDADGTLTLRVMSLGEPDEKGRRRPVETNETVTLHVDSLITAIGEQQDTEALNAMG
                     VPLDKNGWPDVDHNGETRLTDVFMIGDVQRGPSSIVAAVGTARRATDAILSRENIRSH
                     QNDKYWNNVNPAEIYQRKGDISVTLVDSDDRDAFVAQEAARCLECNYVCSKCVDVCPN
                     RANVSIAVPGFQNRFQTLHLDAYCNECGNCAQFCPWNGKPYKDKITVFSLSQDFDNSS
                     NPGFLVEDCRVRVRLNNQSWVLNIDNEGQFNNVPPELNDMCRIISHVHQHHHYLLGRV
                     EV"
BASE COUNT         6737 a         6902 c         7186 g         6478 t
ORIGIN      
        1 tcagaattta ttttagctaa caggtgttca ctggaactat tctcagttac gctggagagg
       61 tatacatgtg cgtgtacgtt tctccggagt aagttatggt tcagaagccc ctcattaagc
      121 agggatattc gctggcagag gaaattgcca acagcgtcag tcacggcatt gggttggtgt
      181 ttggtatcgt tgggctggtg ttgctactgg ttcaggcggt ggatctcaat gccagcgcca
      241 cagcgataac cagttacagc ctctatggcg gcagtatgat cctgctgttc ctcgcttcga
      301 cgctctatca cgccattcct catcaacggg caaaaatgtg gctgaagaaa tttgaccatt
      361 gcgctattta cctattgatt gccggaacct acacgccgtt tttgctggtg gggctggatt
      421 ctccgttagc gcgcgggttg atgattgtta tctggagcct ggcattgctg gggattctgt
      481 tcaaactgac catcgcgcac cgattcaaaa ttttatctct ggtgacctat ctggcgatgg
      541 gctggctgtc gctggtggtg atttatgaaa tggcaattaa gcttgcggcg ggcagcgtta
      601 ccttactggc tgtaggcggc gtggtttact cgctcggggt gattttctac gtctgcaaac
      661 gcattcccta caaccatgcc atctggcacg gctttgtgct cggcggcagc gtgtgccact
      721 ttctggcgat ctatttatat attgggcagg cgtaatgggt gtgcctttgt aggcctgaaa
      781 agacacaccc ttgtcgcatc aggcgataaa tgcatgatgc gacacagaga tttactcttc
      841 gagtgaatac ggcagcggct cgatatgcaa tgtattcgca tcgtcacgca cgcggaagat
      901 gctgtccggt tccatatcgt tattcatgac gacctgtacc acgacctgac catcttccag
      961 ttttaccgca gccagcacgg ttccggtacg acgccagttc tcgcccattt tcagctctaa
     1021 gtcttcaccg gcttccggca gtcggctggc gctacccgtc agcaaccaga gcgcgcgttt
     1081 gttggcacca cggaatttag ctcgcgccac catctcttgt ccggtatagc agcctttttt
     1141 aaagctaatg ccgcccagcg cctggaggtt ggtcgcctgt gggataaact gcccgctgtt
     1201 ggcggcatca atcaccggga aacccgcttc aatgtttaat gccagccact gttggctatt
     1261 gttcagttcc gcttcaccgc gcagtttatc ggtcagcata ttggcagtgg cttcatcggt
     1321 tacgatcagg aaacgttctg ccgggtgttc aaaccatagc aaagtggtcg cgccttcttt
     1381 aactacctgt ttttctttcg aaggcagttc actaaagata tttgccagcg cggcgcgcgc
     1441 ctgaaaaccg gcaacaccaa gcagcacacg ctcgtcgtct ggcgcgatgg tcactttaga
     1501 gaacaccgca tattttttca gttcagtcag ctgcggttca cgcacgctgc gccgttcaat
     1561 ccacgcaaag ccatcgccgt cgcggaacag acgtaaattg ctccacattt tgcctttggc
     1621 gtcgcaatgg gcggcgagca ggtgctgatc ttctgtcatc tggctgacat ctgctgtcac
     1681 ctgaccctgc atatattttt cgctgtccgc gccagtaatg gtggcaagcg cccagtcatc
     1741 aagcgtcatc agcgtcagcg gtaaacgggc agaagccgta ggctgacggg gaggaaaagg
     1801 tgtaaaagcc ataatagagt cctgagttgc ttaacggcgt attaatgact aatggtaaaa
     1861 gagctatgag gcaatgcaag tgctttcact acgcgatctg tgccttctga tatgctttgc
     1921 gaggcgactt ccaaaaaaat gaagcccttg aattgttgcg aattaatttc gcgatcgcga
     1981 ttgtgtttac tgatattccc gcaaaagaag tcgaaaaaca cgttacaata gccgggtact
     2041 ggattttcgt gagacaggaa gaagatggac attaacaaca aagcccgcat tcattgggca
     2101 tgccgccgtg gtatgcgcga actcgatatt tcaatcatgc cgtttttcga acatgagtac
     2161 gacagcttaa gcgatgacga aaaacgcatc tttattcgtc tgctggaatg cgacgacccg
     2221 gacctgttta actggctgat gaatcacggt aaaccggccg atgcagaact ggaaatgatg
     2281 gtccgactca tccagacacg gaaccgggaa cgtggtcctg tggcaatctg atttgcgcgt
     2341 ctcctggcgc gcacagtggc tttccttgct gattcatggg ctggttgccg ctgttatttt
     2401 actcatgccc tggccgctca gttacacccc gttatggatg gtgttgcttt cgctggtggt
     2461 gtttgattgc gttcgcagcc agcgccgcat taatgctcgc cagggggaaa ttcgcctgtt
     2521 gatggacggg cgtttgcgtt ggcaagggca ggagtggagc atcgtcaaag cgccgtggat
     2581 gattaagagc ggcatgatgc tgcgtttacg ttctgacagc ggtaaacggc aacatttatg
     2641 gctggcagcc gacagcatgg acgaagctga atggcgggat ttacggcgga ttttgttgca
     2701 acaagagacg caaagataaa gatgacggca aagcatatgc gctaagccgt cagtgacatc
     2761 aggcgtaatg ctctgccatt tcgttgagga tttgctcgca ccagctctga atacgctcgt
     2821 cgctaaggtc atactggtta gtttcatcca gcgccagacc cacgaacagt tgcccgtcag
     2881 caatcaccgg tttcgggctg gtaaattcat atccttccgt tggccagtag ccgacgaact
     2941 tcacgccttt ggtcgagagt ttgtcatgca gcataccgag cgcatcgagg aaccactcgc
     3001 cgtatcccag ttgatcgcca agcccataca gcgcaacaat tttaccttcg aggttcaggt
     3061 cgtcgagctg atcccagacg gcttcccagt cttcctggat ttcgccaaaa tcccaggtcg
     3121 ggatacccag aatgagcaca tcgtactgct ccattaattt cggggagtcg tccttgaggt
     3181 tatgtaaggt caccagttct gggccgataa tatcgcggat tttttctgcc gccatttcgg
     3241 tgtaacaggt gctggaaccg taaaaaagac ccatattcat aacgtaagct tctcgattct
     3301 gctgtggctt ttgcggctag tgtatcagaa tcgctttagt cacggcacaa taaggcataa
     3361 tggatcaaat gagtgaccat aaggggcgca agtgaaacag gatctggcac gcatcgagca
     3421 gtttcttgat gctctgtggc tggaaaaaaa tctggctgaa aatacgttga acgcttaccg
     3481 tcgcgatctg tcaatgatgg tggagtggtt gcatcaccgt gggttgacgc tggcgacggc
     3541 gcaaagtgac gatttgcagg cattactggc agaacggctg gagggcgggt ataaagccac
     3601 cagctcagcg cgtttgctga gtgcagtgcg ccgattgttc cagtatcttt atcgcgaaaa
     3661 gtttcgtgaa gatgatccca gtgcgcatct cgcttcaccg aaattgcccc agcgtttgcc
     3721 aaaagattta agcgaagcgc aggtcgaacg tttattacag gcaccattaa ttgatcagcc
     3781 actggagcta cgcgataaag ccatgcttga agtgttgtat gctaccggac tgcgtgtctc
     3841 tgaactggtc gggctgacaa tgagtgatat cagcctgcgt cagggcgtag tgcgggtcat
     3901 tggtaaaggc aacaaagagc gcctggtgcc gttaggtgaa gaggcggttt actggctgga
     3961 aacctatctg gaacatgggc gtccgtggct gttgaatggt gtgtcaattg acgtgttgtt
     4021 tcccagccag cgtgcgcagc agatgacgcg acagaccttc tggcaccgca ttaaacatta
     4081 tgccgtgctg gcgggtatcg acagcgaaaa gctgtcaccg catgtgttgc gtcacgcttt
     4141 tgccactcac ttattaaatc atggtgcgga tttacgcgtg gtgcagatgc tactgggcca
     4201 cagcgatctc tccaccacgc aaatttatac gcatgtcgct accgagcgtc tgcgacaact
     4261 tcatcaacag catcacccgc gggcgtgatg tctgaaaaga acaggaaaat ttatgaagaa
     4321 aggttttatg ttatttacct tgttggcagc gttttcaggc tttgttcagg ctgatgacgc
     4381 ggcaattcaa caaacgttag ccaaaatggg catcaaaagc agcgatattc agcccgcgcc
     4441 cgtagctggc atgaagacag ttctgactaa cagcggtgtg ttgtacatca ccgatgatgg
     4501 taaacatatc attcaggggc caatgtatga cgttagtggc acggctccgg tcaatgtcac
     4561 caataagatg ctgttaaagc agttgaatgc gcttgaaaaa gagatgatcg tttataaagc
     4621 gccgcaggaa aaacacgtca tcaccgtgtt tactgatatt acctgtggtt actgccacaa
     4681 actgcatgag caaatggcag actataacgc gctggggatc accgtgcgtt atcttgcttt
     4741 cccgcgccag gggctggaca gcgatgcaga gaaagaaatg aaagctatct ggtgtgcgaa
     4801 agataaaaac aaagcgtttg atgatgtgat ggcaggtaaa agcgtcgcac cagccagctg
     4861 cgacgtggat attgccgacc attacgcact tggcgtccag cttggcgtta gcggtactcc
     4921 ggcagttgtg ctgagcaatg gcacacttgt tccgggttac cagccgccga aagacatgaa
     4981 agaattcctc gacgaacacc aaaaaatgac cagcggtaaa taattcgcgt gaaacaacag
     5041 atacaacttc gtcgccgaga agtcgatgaa acggccgact tgcccgctga attgcctccc
     5101 ttgctgcgcc gtttatatgc cagccggggt gtgcgtagcg cgcaggaact ggaacgcagt
     5161 gttaaaggca tgctgccctg gcagcaactg agcggcgtcg aaaaggccgt tgagatcctt
     5221 tacaacgctt ttcgcgaagg cacgcggatt attgtggtcg gtgatttcga cgccgacggt
     5281 gccaccagca cggctctaag cgtgctggcg atgcgctcgc ttggttgcag caatatcgac
     5341 tacttggttc caaaccgttt cgaagacggt tacggcttaa gcccggaagt ggtcgatcag
     5401 gcccatgccc gtggcgcgca gttaattgtc acggtggata acggtatttc ctcccattcg
     5461 ggcgttgaac acgctcgctc gttgggcatt ccggttattg ttaccgatca ccatttgccg
     5521 ggcgaaacat tacccgcggc ggaagcgatc attaacccta acttgcgcga ctgtaatttc
     5581 ccgtcgaaat cactggcagg cgtgggtgtg gcgttttatc tgatgctggc gctgcgcacc
     5641 tttttgcgcg atcagggctg gtttgatgag cgcggcgtcg caattcctaa cctggcagaa
     5701 ctgctggatc tggtcgcgct gggaacagtg gcggacgtcg tgccgctgga cgccaataac
     5761 cgcatcctca cctggcaggg gatgagtcgc atccgtgccg gaaagtgccg tccagggatt
     5821 aaagcgctgc tggaagtggc aaaccgtgat gcactaaaac tcgccgccag cgatttaggt
     5881 tttgcgctgg ggccacgtct caatgctgcc gggcgactgg acgatatgtc cgtcggtgtg
     5941 gcgctgttgc tgtgcgacaa catcggcgaa gcgcgcgtgc tggcaaatga actcgatgcg
     6001 ctaaaccaga cgcgaaaaga gatcgaacaa ggaatgcaga ttgaagccct gaccctgtgc
     6061 gagaaactgg agcgcagccg cgacacgtta cccggcgggc tggcaatgta ccaccccgaa
     6121 tggcatcagg gcgttgtcgg tattctggct tcgcgcatca aagagcgttt tcaccgtccg
     6181 gttatcgcgt ttgcgccagc aggtgacggt acgctgaaag gttccggtcg ctccattcag
     6241 gggctgcata tgcgtgatgc gctggagcga ttagacacac tctaccccgg catgatgctc
     6301 aagtttggcg gccatgcgat ggcggcgggt ttgtcgctgg aagaggataa attcgaactc
     6361 tttcaacaac ggtttggcga gctggttacc gagtggctgg atccttcact attgcaaggc
     6421 gaagtggtgt cagacggtcc gttaagcccg gccgaaatga ctatggaagt ggcgcagctg
     6481 ctgcgcgatg ctggcccgtg ggggcagatg ttcccggagc cgctgtttga cggtcatttc
     6541 cgtctgctgc aacagcggct ggtgggcgag cgtcatttga aagtcatggt cgagccggtc
     6601 ggcggcggtc cgctgctgga tggtattgct tttaatgtcg ataccgccct ctggccggat
     6661 aacggcgtgc gcgaagtgca actgacttac aagctcgata tcaacgagtt tcgcggcaac
     6721 cgcagcctgc aaattatcat cgacaatatc tggccaattt agcgtcatct tctctataaa
     6781 aaagagcgtg gattgggtac aatcccgctc ttatcaccgc attttgacta gctcaataaa
     6841 agaaatcaga ccatgtttga aattaatccg gtaaataatc gcattcagga cctcacggaa
     6901 cgctccgacg ttcttagggg gtatctttga ctacgacgcc aagaaagagc gtctggaaga
     6961 agtaaacgcc gagctggaac agccggatgt ctggaacgaa cccgaacgcg cacaggccct
     7021 gggtaaagag cgttcttccc tcgaagccgt tgtcgacacc ctcgaccaaa tgaaacaggg
     7081 gctggaagat gtttctggtc tgctggaact ggctgtagaa gctgacgacg aagaaacctt
     7141 taacgaagcc gttgctgaac tcgacgctct ggaagaaaaa ctggcgcagc ttgagttccg
     7201 ccgtatgttc tctggcgaat atgacagcgc cgactgctac ctcgatattc aggcggggtc
     7261 tggcggtacg gaagcacagg actgggcgag catgcttgag cgtatgtatc tgcgctgggc
     7321 agaatcgcgt ggtttcaaaa ctgaaatcat cgaagagtcg gaaggtgaag tggcgggtat
     7381 taaatccgtg acgatcaaaa tctccggcga ttacgcttac ggctggctgc gtacagaaac
     7441 tggcgttcac cgcctggtgc gtaagagccc gtttgactcc ggcggtcgtc gccacacgtc
     7501 gttcagctcc gcgtttgttt acccggaagt tgatgacgat atcgatatcg aaatcaatcc
     7561 ggcggatctg cgcatcgacg tttatcgcgc gtccggcgcg ggcggtcagc acgttaaccg
     7621 taccgaatct gcggtgcgta ttactcacat cccaaccggg atcgtgaccc agtgccagaa
     7681 cgaccgttcc cagcacaaga acaaagacca ggccatgaag cagatgaaag cgaagcttta
     7741 tgaactggag atgcagaaga aaaatgccga gaagcaggcg atggaagaca ataaatccga
     7801 catcggctgg ggcagccaga ttcgttctta tgtccttgat gactcccgca ttaaagatct
     7861 gcgcaccggg gtagaaaccc gcaacacgca ggccgtgctg gacggcagcc tggatcaatt
     7921 tatcgaagca agtttgaaag cagggttatg aggaaccaac atgtctgaac aacacgcaca
     7981 gggcgctgac gcggtagtcg atcttaacaa tgaactgaaa acgcgtcgtg agaagctggc
     8041 gaacctgcgc gagcagggga ttgccttccc gaacgatttc cgtcgcgatc atacctctga
     8101 ccaattgcac gcagaattcg acggtaaaga gaacgaagaa ctggaagcgc tgaacatcga
     8161 agttgccgtt gctggccgca tgatgacccg tcgtattatg ggtaaagcgt ctttcgttac
     8221 cctgcaggat gtgggtggcc gcattcagct gtacgttgcc cgtgacgatc tgccggaagg
     8281 cgtttacaac gagcagttca aaaaatggga cctcggcgat atcctcggcg cgaaaggtaa
     8341 actgttcaaa accaaaaccg gcgaactgtc tatccactgt accgagctgc gtctgctgac
     8401 caaagcactg cgtccgctgc cggataaatt ccacggcttg caggatcagg aagcgcgcta
     8461 tcgtcagcgt tatctggatc tcatctctaa cgatgaatcc cgcaacacct ttaaagtgcg
     8521 ctcgcagatc ctctctggta ttcgccagtt catggtgaac cgcggcttta tggaagttga
     8581 aacgccgatg atgcaggtga tccctggcgg tgccgctgcg cgtccgttca tcacccacca
     8641 taatgcgctg gatctcgaca tgtacctgcg tatcgcgccg gaactgtacc tcaagcgtct
     8701 ggtggtcggt ggcttcgagc gtgtattcga aatcaaccgt aacttccgta acgaaggtat
     8761 ttccgtacgt cataacccag agttcaccat gatggaactc tatatggctt acgcagatta
     8821 caaagatctg atcgagctga ccgaatcgct gttccgtact ctggcacagg atattctcgg
     8881 taagacggaa gtgacctacg gcgacgtaac gctggacttc ggtaaaccgt tcgaaaaact
     8941 gaccatgcgt gaagcgatca agaaatatcg cccggaaacc gacatggcgg atctggacaa
     9001 cttcgactct gcgaaagcga ttgctgaatc tatcggcatc cacgttgaga agagctgggg
     9061 tctgggccgt atcgttaccg agatcttcga agaagtggca gaagcacatc tgatccagcc
     9121 gaccttcatt actgaatatc cggcagaagt ttctcctctg gcgcgtcgta acgacgttaa
     9181 cccggaaatc acagaccgct ttgagttctt cattggtggg cgtgaaatcg gtaacggctt
     9241 tagcgagctg aatgacgcgg aagatcaggc gcagcgcttc ctggatcagg ttgccgcgaa
     9301 agatgcaggt gacgacgaag cgatgttcta cgacgaagat tacgtcaccg cactggaaca
     9361 cggcttaccg ccgacagcag gtctgggaat tggtatcgac cgtatggtaa tgctgttcac
     9421 caacagtcat accatccgcg acgttattct gttcccggcg atgcgtccgg tgaaataagc
     9481 attacgttat gctcacaacc ccggcaaatg tcggggtttt ttaattgtgc tgcgcgaaag
     9541 cagacaacaa ttttcttgct tcactattgg ctgcctgcat caccatccac ggactgaacg
     9601 cccacggcgt ggcatcaata ccgtgtaata catctgctaa atcacaccat tgataatcca
     9661 tcacttcatc atcgttgatc tgtaacgcac tggtggtgcg tgcggcaaat accggacaca
     9721 cttcattttc cacaatgcca ttcggatcgg tggcgcgata gtagaagtca ggatagatag
     9781 attcaggagg cgtaatttcc acgccaagct cataacggca acggcggatc accgcgtctt
     9841 cgttgctttc tcccagttgt gggtgcccac aaaccgagtt agtccacacg ccaggccacg
     9901 cttttttgct aagggcgcgg cgggtaacta ataattgccc tttggcatta aacagccaac
     9961 tggagaacgc gagatgtaag agagtgtctg ccgtgtgtgc ggcatacttt tccagcgtac
    10021 ccgtgggaac tccctgtgca ttcaataaaa tgacgtgttc cgtttgcata atttctcaca
    10081 tgtaattcta atcactcatc attatagata attgaagagt gaaatgatta accaagtgag
    10141 cgtttatcga caaccgcccg ttttgagcgg atgtcgacag gtaaaaacca tttaaaaaag
    10201 tagcatcata tatttgaagc gactttcaat ggccattgca acacagtcag aaatgacagg
    10261 aatagagaga gtaaaaagca acagcagact gaatatagcg cgcattaaaa gattcatatt
    10321 attctccatg ctcatttttc ttcagcttgc gcatcagaag ccacatacgt actgtcctga
    10381 cttttaaatc tttatcagag acggattcct tcaccagatc agcatgatgc tgatagccat
    10441 taaagaagac gttcagcaca acggcgctaa gggttgcgag cataatgccg ctatgcagca
    10501 acggttgcag tacggccggt aacttagaaa agaaatcgtg agagagcgtc ggagtcatgc
    10561 caacgccgag actgatcgcc acaatataga ggttgtaacg gttggtggtg tagttacagc
    10621 gcgacagaat tcgaatccct gtcgccagta ccatgccgaa catcaccaga ccagcgccgc
    10681 ccagcacaaa ttgcggaatg gaggcgacca gcaccgccat ttttggcacc atgccaaaca
    10741 ggattaatat aattcccgaa gaaatacaca cccagcggct atgaacgcgc gtcacgctaa
    10801 ccaggccaac gttttgagaa aatgacgtat gggggaagct gttaaacgtt ccgcctatca
    10861 ttgtccctac gccatcgaca cgcagcccgc gaataatatc gtgtgaagag agtttacgac
    10921 caactatttc acccagtgcc aggaacatcc ccatcgactc gataaacacg atgattaaca
    10981 cggcagtcat ggtcagaatg gaaacgggat caaaaatcgg cataccaaac gacatcgggg
    11041 tgacaatcgc aaaccacgag gcatcatgta gcccggataa attgacttca ttcatcatcc
    11101 acgaaagtaa aaagccaaat acaatcccca gtaatacggc gacgttggac ataaatcctt
    11161 tcgcatagcg agtaatgagc aagataaaaa ttaagacggc aaaggagata cctaaataaa
    11221 cgggattacc atattgcgga ttccctttac ctcctgcggc ccagtcaata cccacctgaa
    11281 tgatgctaag tccgatagaa gtaataacca caccggtaac cagtggcggg aataaaggca
    11341 tcaagcgacc gataagtggc gctaataatg tggtgataaa acccgcggcg atagtggcac
    11401 caaatatccc cagcaggccg atatccgggt tcatcccaat ggctatcatt ggtgttacag
    11461 cggcgaaggt caccgacata atcaccggca ggcggatccc cataaagcgg ccgataccga
    11521 tacattgcaa taatgtgacg atcccgcagc aaaagagatc cgagctaatg agcatcgcaa
    11581 tagcttcttt tgagaggccc agtcggtcgc caatcataag aggaacagcg actgcacctg
    11641 cgtacatgac aaggacgtgt tgcagaccga gtatgatcag ctttcctggt gataatatgc
    11701 ggtcaacctc atcagttggg cggtcttgcc cagaagatga gggaagttgg gaatctatgg
    11761 cgctcatcag tgtttctcct ttcgcccgcc agagtaaaaa gcaaccgttt acgttcatga
    11821 aactgtctaa atacatgctc gtggagtgcc agttttgcga ggaaggggag ttagagacag
    11881 gaattgcgga ggtaatcaca taatcgtggt taaaccacga aaatgagaag agaacctgac
    11941 aatggaatta tctgtaattt tctcatattg atatttcatt aatcattctg gcatgtcgca
    12001 ctctcgcatt taatcgtttt tatctggata gcattctttt gatcggcaat gttgaaataa
    12061 tgaaagctgc atagattgag gggcaataat ttgaactcag tcgcaaaatt ataattaaat
    12121 cggccatgct acctaataca tgcccattta ttgcatcgcg aaatttgaga agacataact
    12181 cattaaatga aagggatgca aatgaataag tttatcgctg ctgaagctgc ggaatgtata
    12241 ggctgtcatg cttgtgaaat tgcctgtgcg atggcacata atcaagaaaa ctggccgctg
    12301 agtcacagtg actttcgacc gcgtatccac gttgtaggga aaggccaggc tgcgaatccg
    12361 gtggcctgcc atcactgcaa caatgcccct tgcgttacgg cttgtccggt taatgctctg
    12421 actttccagc ccgatagcgt acaactggac gagcaaaaat gtattggttg taaaagatgc
    12481 gcaatcgctt gcccctttgg cgtcgttgag atggtcgata cgattgcgca gaaatgcgac
    12541 ctttgtaacc agcgcagttc cggcacgcaa gcctgtattg aagtctgccc aacacaggcg
    12601 ttacggctga tggacgataa agggttgcag cagataaagg tggcccgcca gcgcaaaacg
    12661 gcagcaggaa aagcgtcatc agacgctcag ccatctcgca gtgcagcgtt gctccccgtt
    12721 aactcgcgta aaggcgcaga taaaatttca gcgagtgaac ggaaaaccca ctttggcgaa
    12781 atctattacg ggctggatcc acaacaagcg acttatgaga gtgaccgctg tgtttattgc
    12841 gccgaaaaag ctaactgcaa ctggcattgc ccgctgcata acgctattcc ggattacatc
    12901 cgtctggtgc aggaaggaaa gattattgaa gcggcagaac tttgccacca gaccagttcc
    12961 ttgccagaaa tctgcggcag ggtatgtcca caggaccgtc tttgtgaagg cgcatgtact
    13021 ttgaaagatc actctggcgc agtctctatc gggaatctgg aacgctacat taccgatacc
    13081 gcgctggcga tgggctggcg tcccgatgtc agcaaagttg ttccccgtat cgaaaaagtg
    13141 gcggtgattg gcgcagggcc tgcagggtta gggtgtgctg atattttggc gcgcgcagga
    13201 gttcaggttg atgtctttga tcgccatcca gaaattggcg gtatgctgac ttttggcata
    13261 cctcctttca aactcgataa aacggtatta agccagcggc gagagatatt caccgcaatg
    13321 ggaatcgact ttcatcttaa ctgtgaaatt ggccgcgata ttacctttag cgatttaact
    13381 tctgaatatg atgcagtttt tatcggcgtg gggacttacg ggatgatgcg agcagatctg
    13441 ccgcatgaag atgcgcccgg tgtcattcag gcgttaccgt tcctgactgc ccatacccgc
    13501 cagctcatgg ggttgccgga gtctgaagag tatccgctga cggatgtgga aggtaagcga
    13561 gtcgtggtat tgggcggtgg cgatacgacc atggattgtt tgcggacttc catccgcctc
    13621 aatgccgcca gcgtgacctg cgcgtatcgt cgtgatgaag tcagtatgcc gggctcgcgc
    13681 aaagaggtgg tcaatgcgcg cgaggaaggt gtcgagtttc agttcaatgt tcagccgcaa
    13741 tatatcgctt gtgacgaaga tgggcgttta actgcggtgg gcctgattcg taccgccatg
    13801 ggtgagccgg ggccggatgg tcgccgtcgt cctcgtccgg tagcgggttc agagtttgaa
    13861 ttgcccgccg atgttctcat tatggccttt ggtttccagg cgcatgccat gccgtggttg
    13921 cagggcagcg gaattaaact cgataaatgg ggcctgattc aaaccggtga cgtcgggtat
    13981 ttacctaccc agacgcatct gaaaaaagtc tttgctggtg gtgatgctgt tcatggcgcg
    14041 gatctggttg tcactgcaat ggccgcagga aggcaggcgg cgcgcgatat gttaactctg
    14101 tttgatacga aggcatcatg atgaaatcgt taattatcgt taatccggct gactgtattg
    14161 gctgccgtac ttgtgaagta gcctgtgtgg tcgctcaccc ttcagaacag gagttgaatg
    14221 ccgatgtctt tttaccccgg ctgaaggtac agcgactgga cagcatcagt gcgccggtga
    14281 tgtgccatca gtgtgaaaac gccccttgtg ttagcgcttg ccccgtgggg gcgctgacga
    14341 tgggcgagca ggtggtgcag accaattctg cccgttgtat tggctgtcag agttgcgtca
    14401 gtgcgtgccc gtttggaatg atcaccattc agtcgttgcc gggggatacc cggcaacaaa
    14461 tcgtgaaatg cgatctctgt gaacagcggg aagaggggcc agcctgcgtt gaatcttgcc
    14521 caacacaggc gctgcagttg cttactgaaa gagaactcag gcgagtccgc cagcagcgta
    14581 ttgttgccag cggtgagaat cctctgtgaa agaaatctcc gcgctgaaag gcgcggagga
    14641 tctattagat tgcccaacca cccgcgtaaa atgcgaccag tgcggcagta atgatcaccg
    14701 taccaatatt cagcttttgc cattcgcgtg caaagacgcg gcctacgacc agtgtcacaa
    14761 agccaagcat aataccggta acgatattac aagtcagaac gatgaacacg gcacacacca
    14821 ggccagccat tgcgtcaata aaatcattga aatccagctt cgagacgtta cttaacatca
    14881 gcaaacctac gtacatcaga gcgggggcag tggcgtaacc agggatcaaa aatgacagcg
    14941 gtgataagaa cagaatcagc aggaataacg cccccactac ggttgcggtt aaaccggttt
    15001 tccccccggc ggcggttcct gccgctgatt cgatataaac cgccgcgggc gctgcgccca
    15061 ccaggccgga gaatattgaa cttactgagt cactggtcag ggctttgccg ccgttgatga
    15121 tctggttgtc tttatccaac aaattcgcct gaccggcgac ggcacggatg gtgccagtgg
    15181 cgtcgaacac tgcggtcatc accaatgcca gtacactcgg aagtacagtt ggctggagtg
    15241 cgcccataat atcgaggctg aaaatcagag acttaccatc ttcgccagtc aggcttggca
    15301 tcgccaccag accgtggtac ttcaccgctg ggtcaaagat taagccgatg atcgaaattg
    15361 caataatcac caacaagatc ccgccgggta cgcgacactt ctccagaccg aagataaccg
    15421 ccagccccag caagctcatc atcaccggga aggaggtaaa cgcaccgagc gccactggca
    15481 agccttcaat cgggtttttg ataaccattc ccacaccgtt agcagcaatc agcagcagaa
    15541 acaggccgat accgatacct gtaccgtgag caatccccat cggcaaatta cgtaagatcc
    15601 aggtacgcac accggttacg gaaatggcgg tgaagatgac gcccatcaga aataccgcgc
    15661 ccagtgcgac aggaacgcta atttgttgcc cgagtaccag actgaatgcg gtaaacgccg
    15721 tcaaggaaat cgcacaacca atcgccattg gcaaattggc ccacaacccc atcagcaacg
    15781 agccgaagcc cgcgaccaga caggtggcaa caaacacagc tgcgggagga aaacctgctt
    15841 tgcccagcat tcccggaacg acgataacgg aataaaccat ggccagaaag gtcgttaagc
    15901 cagccagtac ttcctgacga acggtactgc cacgagcggt aattttaaaa taattatcca
    15961 gcgcgccctg tggctttggt gcgtccggtg tttgtaggat gtctccagac ataatgatgt
    16021 cctcacgagg atgttaaagt tttattatta gttgcgttcg tacaccagac gaccatcaac
    16081 gtaggtgcgg tagatcgaac ggtcatcgcc caacgtcatc atcacgaaca atttgtcgac
    16141 taaagaaaca gagttgtcat agcgcagctg ctgtagcgga gtggcagtgg gttccatcac
    16201 cacgaaatca gcctctttgc caggtaaaaa gttgccaatc aaatcgtcaa ggcccagaga
    16261 tttcgctccg ccgagcgtgg ccaggtaaaa cgcttcatat gccgagaggc gatagccttg
    16321 taattgcaat accttgtagg cttcgttcag cgtttgcagc atgttaaaag ttgttccggc
    16381 accgatatcc gttcccatgc ccactttgac tttcttctgc catgcttttt tcaagttgaa
    16441 taagccgctg ccgaggtaaa ggttggaggt cggacagaaa gcaatgctgg atttggtttc
    16501 gctgagacga tcccactctt tttcttcgag atggacgcag tgagcaaaga cacagttttt
    16561 accggtcaga ccgtactgat ggtaaacatc cagataacca tcatggtcag gataaagcga
    16621 tttgacccag gcaatttcat ctttgttttc acagagatgg gtatgtaccc acgtatccgg
    16681 atattcttct ttcaggcgtt gcgccatcgc catctgttca ggagatgagg taggggcgaa
    16741 gcgtggcgta atcgcataca acagacgacc atttttgtgc cagcgttcaa tcagttcttt
    16801 gctttggtga tagctgcttt cggcagtgtc gagcagataa tccggtgcgt ttcggtccat
    16861 catcacctta ccggcaatca tacgcatatt gatatgactg gcggcttcaa acagcgcatc
    16921 aacggattgt ggatgaacag tgccaaacac cagcgccgtg gtggttccgt tacgtaaaag
    16981 ctgcttgatg aagaacgccg acatctcgcg ggcgtactct aaatcctcat aacgacgttc
    17041 agtagggaag gtgtgtttat tcaaccactc cagcaattgt tcaccatagg cccccaccat
    17101 ttcactttgc ggataatgga tatgtgtatc gacaaagccc ggtactatca gtttgccgcg
    17161 atagtcgcgc acgcgaatgg tgtcaggaat ttgatgcttt ccgtcttccc attcgccaaa
    17221 ccattccact tttccctgtt taatgagtaa taaaccatcc tcaataaacc gcagcgcaga
    17281 ggcaatttct tccgggttat cgaccgtacg ggtgacatca ataaaactgc ctcgtaccgc
    17341 ttttaacgtg tgttctcctg acatcattga ctcctttact gttaatccat ctcttccgct
    17401 gaggtaatac caggcagaac gttttcctgt cggtagccac cagggaggat aatatttaat
    17461 aaaatcgcag ttaacccgcc agcacaaata gggttttcaa ctaatacata aatagaggct
    17521 ggcaatattt taaaaatttc gggatcgtag gagacgccaa gccctaaacc taaagacgtg
    17581 gcgacaataa gtgtttctcg gcgctttaat ccgttggtga tgatgatgcg aatcccagcg
    17641 atggcaatca tggaaaacat caacgtcatt gcgcctccca gaactgccga gggaatggtc
    17701 gtgaagaagc cgccaatcat cggaaataag ccgaggataa ccagcattac cgcgatggtt
    17761 cgcccgacat aacgtgaagc gacgccagtc atctgaataa ccccattatt ttgcgcaaac
    17821 gtggttaatg gtaatgaacc gacagcggag gcgataacag aaaccagacc atctgccagc
    17881 acgccgcctt tcagtcggga ctgatactct tccccctgaa tggggcggcg ggaaaccatt
    17941 gccgtggcgg tgatatcgcc gactgcttcc agcacgctaa gcagataaat cgtgccaacc
    18001 accaggaact gatggaagct aaaactaaag ccgtatttga acggatgcgg gatggtgatt
    18061 aacggcaaat tgcgcatact gctgaaatcc accatgccca ggcataacga tgcgatatag
    18121 ccgacacata gcccaatggc gatccctccc atgcgtagca acggactgcg acagcagtta
    18181 aagccgatca ccacaattaa aaccaataaa ccaacgccga gatgttcgta attgccgaac
    18241 gtaccgctgc ttttggctgc aaatcctcca ccaaaatcga taatgccgac tttaatcagg
    18301 cttaagccga tcatcagtac cacaataccg ctgacggtag gcgtaataac ccgacgtaaa
    18361 tagggcagga taaaagaaga gccgacaacc agaaatgcgc caacgaagga gacgccgagg
    18421 agcgacgaca tgattaactc ttcgtgaaaa ccgtcgcttt tcatgctgct gcccagcgca
    18481 atcatgaccg taacaaatga aaaattgact gactgaattg agagaagacc agaaccgacg
    18541 atgccgtagc ggtttacttg taaccaggta ccaataccag aggcgatcat cgccatagaa
    18601 acaagatagg cagttgtttc agcggaaagc tgtaaggtcg cacccacgat taacgcgggg
    18661 gttaccatcg gaacgaaaat tgccaacaga tgggtaatgg cacctacgag agcctgatga
    18721 aagggagggc gatcctccag ttcaaatata aggtcagaac ctgcatggtt tatatcagac
    18781 atcccttcac tccttttaaa gtaaatttga tcatctgaat tagtaaaata aattcccacg
    18841 aatttggggc gcaggacgcc ttgagaacgg cttatctcat tttcgcagtc actatttagt
    18901 catcgttcaa acagagaaac aggccgccga agcggccatt tattacttat attttttcca
    18961 gcgcggtgag tattttctcc ggtgtgaaat gccattcgcg taaccagatg ccgcaggcat
    19021 cgtgaattgc ggtagcaatc gccggagcgg cgccatttac accgatttcc gagatcgatt
    19081 tcgccccgaa tgggccgact ttatcgtcgc tcggcaccag cacagcgcgg aaatcacgcg
    19141 gaatgtcacc aattttcggt gcgccgtaac tgcgtaaatc acgcgttaac gggtgacctt
    19201 cggcgtcata aatgatctct tcgctcatac tgtggccgat agctcgcaga gtggcaccgt
    19261 agatttgtcc caacgctaac tctggattga ccggtgtacc gcagtccagc aaggcgtaga
    19321 atttatccag gcggatttca cccgtacgcg tgttgacggc aacttcagcg aagtttgcgc
    19381 catacgggaa ggcgaaatca ggcgtgatat aactgccagt tcccaccagt gaaccaaagc
    19441 cggtgccggt ttcgccttta tgggctatct ctccgaaact aacttcgcct ttcttgccgc
    19501 gtacgacgcc cggcgttgct agttggacat ctgccactgg ctcacccaac atttgcgcac
    19561 cgtggaacag aattttctcc cgcagatttt ctgctgccaa acgcgccgcg ttacccgaga
    19621 agcaagtacc ggacgaggca tatgcgcctt tatcaaacaa cgcatgatcg gtatcaccgg
    19681 agataacatg cacgtcctgc ggtgggcagt gcagcacttc tgctgccagt ttcgtcacca
    19741 cagtatccag accagtacca atatccgcac cgccagaatg aacgataaag gtaccgtctg
    19801 attccagttt gatcatgcag ttagcctgat cgatatctgg gatccctgat ttctgcatga
    19861 taatggcgac gccacgaccg atgtgccagt caccgttttg tggttttggt gaagaccatt
    19921 ggatcatctc gcgaccctga cgcaggatct cttccagtgc gcagctggcg gcggaaggaa
    19981 cggaggtcgg cgctttacct tcaccgattg caccgagaat tttcagctct tgcccttcgt
    20041 gtacccggtt acgttcgata atttccagtt ggtcgatctg taactgttca gccagttccg
    20101 ctaatgccat ggtgatagcg aagttacctt tcggtgcgcc ataaccctga taagcaccat
    20161 ttgggcaaat gttgctgtag taggtggtga cctggaaatc gacgttatcg cacggatata
    20221 acggcagcga cagcgccggt ccgttacatg gtacggtgag tgagtggttg ccgtaagggc
    20281 cagtgttggc gcggaaatcc atcttcactg ctgtcaggcg accatctctt ttcgctccca
    20341 gtttgacggt gactttcgca acgtgacgag aggtgttagc aataaactct tcttcacggg
    20401 tgtagcggaa cagtaccgga cgcccggtca cgcaggttgc ccaggcgcac acttcttcca
    20461 gcaggatgtc ctgtttagac ccaaaaccgc cgccaactcg ctctttaatg acatgaactt
    20521 tatgttgttt catgcccacg aggcgcgcga cctggcggcg taagtgccat ggtacctggg
    20581 tggaggcgtg gatcaccaga cgatcgccgt ccatacgggt aaagcagata tgtgtttcag
    20641 tcgggcactg ctgtgcctgc gttgagttat aggttcgctc aatgatcaca tcggcatcgg
    20701 caaagccttt gtccatatca ccaatatgac catgaatact ggcagcgata tttttgcgtg
    20761 ggcgagaacc gatcgggaag ttgatgatca tatgctcgcc gcgctgggct gcatgacagt
    20821 tatcttcctc cagagtatct ggtgcaccag caacatacac caccggttca tcgtgcacga
    20881 caggcgcatc ttccgccatt gcctcgtcaa tcgacattac cggcttaagc acttcatatt
    20941 caacgtcgat gagcttcagt gcttcgagcg caatgtcttc actttcagcg acgaccgcag
    21001 caacgcgatc gccgacgtga cgcattttct tgccgaacat tcggcggtca agcggtgacg
    21061 gttccggtgc gctctgacca cccggggtat agtaaatatc cgggcaattc aggtgagtaa
    21121 taacgtgaac gacgcccggt aaggcttcag ctttgctgac atccagatga gtaatcagtg
    21181 cgtgagcgtg tgggctacgt aacattttaa tgacgcaggc gtcagccgtt acgcgatctt
    21241 caacatagca gggtttcgcc tgcaccattt tcgcggcatc agttttagga taatgcttgc
    21301 caatgacttc aaggtcgtca cggaaagtcg gagcgatatc aatggtggcc tgcggattat
    21361 ttttacgtgc aaccgccagt tcaatgacct gatagtattg ctgccagcca gcatcgcggc
    21421 taaacagacc tgagagcgcg tcgtcgatct cttcgcgggt aggtgcgtcg atgcgatcga
    21481 gaagatcggt gataatcaga gccgcagctg gatcgttata accagactgc accacgccaa
    21541 catcaaccat ggcctgttga accagactta actcattcca tttgcccaga gattctgcgg
    21601 tacgaatatc tgccttctct aactgtgcgg cgataagcaa ggacgcgtta acgatattac
    21661 cgttaaagat aattgcgtca gaaccggcaa acccaaagcc atcatcgctg ttgcgtacag
    21721 agtgcattcc catgttaaac aacagctttt gcacgttttc accaggatta acggttagct
    21781 cctggggcgt gccatttaaa gtaaagtgga tgatcatacg gcttcctccc cagcctgttg
    21841 gcagtcggca taaaggtcgg ctaccagaac tcccgtgata tagcgtttat aggccacgct
    21901 gccccgcaaa tcttcctgcg ggaaaatggc gttggcgaca gcctgttcaa gggcatcacc
    21961 ttccaggttt tgtttttcaa catcgtgcag acgcagtgct ttactggcta cgccatccag
    22021 cgcaattcgc ataccgtcgt ggtctgttat tgcaacggct gccgtcacga cggttaaacc
    22081 tgcctgagaa cggctaattt tgcgagtcgc acaggtgcga tacggatctt taatgatgat
    22141 ttcggttagc aggcgatcgc atgggcaggc caggtactcc tcaattgaca gagtttcgcc
    22201 gttgccaaaa accagttcag catccagtgc cagcaggacg ggaagcagca ctgactcttc
    22261 ttgccgggcg gcaatttcac caccaatggt cgactgatta cgaacatggc gtgagtaaac
    22321 aaaaccgaga gcttcacgca gtgctgcagg aataaatcgc gcatcacgca gtgtttgcaa
    22381 gcgagacatc gcgccaatcc gcagtgcacc gttatcccag tcaacccagt ccagttccag
    22441 atcctgtaag gaaatggcaa tctttttatc ggtacgggtt ggtgtagcgt tgagtttgct
    22501 gcccccggcg aaccagacgg cttcatcctg gtagcggcgc ttcagttcca gcgcctgttc
    22561 gacagagtcg ggtctgaaaa attgttcaat catcgtcttt ccttaatttc ccctcttctc
    22621 tgaaagaaga ggggagctgt tatttatgcc agcgcatcca tccgacgcca catactggca
    22681 gcggcttttc tggcttgcgc ataaatggaa tcgcaatcga agttaaactg acggtcttca
    22741 tagaccatca caccattgac catcacgctg tgaacgctgc ctgagcccat accgaaagcg
    22801 atatgcccgg cgatattgtc tgccagcagc ggcgtcggcg agttgtaatc gcaaatggtt
    22861 aaatcggctt tgtaaccggc ttccagaagg ccaaatttcg cgccaaagtt gcggctcatc
    22921 agttcgttgc cgttagccag agctttggca aagctgtcag gccacagcgg accacccgca
    22981 tcgcgatgtt taaagaaggc aaatttcatc tcttcaaaca tatctgaacc aataccgtcc
    23041 gtacccaacg ccagattgcg gatgtcgcaa agatgatggt tgtagccgac atggttgttc
    23101 atgtttgaac gggcgttatg caccaggaat gcatcacgct gattgagcaa cgcgatgtcg
    23161 tctttagaca gataaagccc atgagcgacc agcgttttgc tgtcgatgag atcgaattgc
    23221 gctaatcgtg ccagcagatc tttgccgtac cagtgatgac tatgggaaac atcgtaaagg
    23281 tcttccgcag cgtgaatatg taaaccacgg cctgtggctt tcacggcctc acgcagcatt
    23341 tccagaccgg catccggcac agtaaacggc gcgtgagcac caatatgagc ctctaccaga
    23401 tacggttcag ttgccgcttt cttcgcatca tcaatctgac gggcgaagcg gatgttttct
    23461 tctacacctt cctgcaactc tttgataccg ttgttacggt cagtagtttc aaaacaggtc
    23521 atcgcgcgca ggccaacttt taaaaatgcg ttgcgcaatg tggagagcga cccgtcgata
    23581 tacgccggag aggcatggtg atcgataacc gatgtacatc cgcccttaat cgcttccagg
    23641 gaacaaatca gtccgctgta atagagcgac tcttcatcaa gggcgcgatc gagtcgccac
    23701 cagagatttt tcagcgttga gatgaaatcc gggcaggggg cgatgtttgc cataattccg
    23761 cgggacagcc ccgagtaaaa atggttgtgc gagcagacaa ttcctggcat cacaatccga
    23821 ccatgcatct ctttgtagct ggcatcgggg tagcgttgcg tcagggcatc gccgatagcg
    23881 acaatcacat cattttcgat ggcgatatca acgccttcct gcactttcgc tgggtgtaac
    23941 tgcactgcgg tgacattctt cagaatcaac atgattacac ctccacacgg cccagcagat
    24001 aatgatgatg ctggtggaca tggctaatga tgcggcacat atcgttcagc tccggtggta
    24061 cgttgttgaa ctgaccttcg ttgtcgatgt ttaacaccca gctttggtta ttcagacgta
    24121 cgcgtacccg gcaatcttcc acaaggaagc ctgggttgct gctgttatca aagtcttgcg
    24181 acaggctgaa gacggtgatt ttgtctttgt acggtttacc gttccacggg cagaattggg
    24241 cgcagttgcc acattcgtta cagtaagcgt cgaggtgcag cgtctggaaa cggttctgga
    24301 agcctgggac cgcaatggat acgttggcgc ggttcgggca gacatccaca cacttgctgc
    24361 aaacgtagtt acattcaagg cagcgtgcgg cttcctgggc gacaaacgcg tcacgatcgt
    24421 cgctatccac cagggtgaca gagatatcgc ctttgcgttg atagatttcc gccggattaa
    24481 cgttgttcca gtatttatcg ttctggtggg aacggatatt ttcccgacta aggatggcat
    24541 cggtcgcccg acgcgcggtt ccgacagcag cgacaatgga ggatggtccg cgctgtacgt
    24601 cgccgatcat aaagacgtca gtcagacgag tttcgccatt atggtcgacg tctggccagc
    24661 cgtttttgtc cagcggcacg cccatcgcat tcagggcttc agtatcctgc tgttcaccaa
    24721 tggcggtgat caggctgtct acatgcagtg ttactgtttc gttggtttca accggacggc
    24781 gacgaccttt ctcatcaggt tcgccaagcg acataacgcg caaggttaag gtgccatcag
    24841 catcgaaacg ttccggatta ttcagaaaac ggaactccac gccgtcgtgc aacgcttctt
    24901 catactcttc gcgccatgct ggcatctctt gcagtgaacg acggtaaacg accgttgctt
    24961 tttctacgcc tggaacgcgt aacgccgcac gagcgcagtc cattgcggtg ttacccgccc
    25021 cgacaacgac cacatgtttg cccagcttga gctctgtgcc cttgttgtat tcacgcagga
    25081 aggggagtga tttccagaca ttttggttgt cgcccgccag tttcacaccg ctatttttat
    25141 cagtgccggt ggcaatcaga acatagtgga agccctgatt ttttaactgt tcaacggtca
    25201 gatcgggtga gcagccatac tcaaatttca cgccgtgagc ggcaacaaaa tcgatatcgt
    25261 gctgaattaa ctctggagga atacggaact gaggaatgat atttttcacc acgccgcccg
    25321 cgttggcttc gcgttcaaac agcgtaaccg gatggcccgc tctggcaagg aagtaacctg
    25381 cggccagacc cgccggacct gcaccaatca cggcaaccgg atggcgtgaa ccagaaccgg
    25441 ctggtttgtg ccagcgttgc ttatattcat cccaaccttt ttccagcgcg acttttttca
    25501 gttcgcggat attcagcgca ctgtcgtaat ccaggcgggt acagttgtat tggcactggt
    25561 gatcgcaaat atgaccggta atggcgggca gggcgttgcg ttgatagatg agttccagcg
    25621 cgtcggcata gcggtgttcg ccaagcagac ggatgtattc cggaatatct tgcttgatag
    25681 cgcaggcagt aacacagggg gcaacgtagc aatcggtcag cggcaggtct tcagccactt
    25741 caatacgctc ttctggcttc cagtgtttct gggtgtattc catggttaac gcgtctgctg
    25801 ccagtctgtt cagtcgttcg acgtcaacgt ggtcaagtcc ccaggcgtcg gagccttcca
    25861 gctcgcgcat gcaggcactt aagcgcagat agccgccagg tttcagcagg tcggttgcca
    25921 tagtaatagg gcgaatacca gtatcaaaaa tatcgcggat agtcagctga ctggcaccac
    25981 cggaataaga aatgggcagt ttgccgtcaa aggcgcgaga gagaactgct gcaacgttga
    26041 tggagagcgg gaacagcgca cggcctgaca tatacatctc ttcaccgggc agtgcgcctt
    26101 tgttattaat ggtgccgaga gtgttagtca gttttacgcc aaagccgagt gatttttctt
    26161 ttgccagtgc catcaggcgt tccagcattt ccagtgcttg cgtcagcttg aggtcgtgat
    26221 caaatgactc ttcttttaag ccgatgtaac cgaaaccgca gacatcgagg atctcacgaa
    26281 cacgcgcgta ccccagtaag gtcgggttga gtttcacaaa ggtgttgagc cctttttctt
    26341 ccagcatgta gcggcaaatg gcttcgattt catgcggagg acagccgtgc atggtggaga
    26401 gggtaacccc ttgtaccatg ctggtgggga tgcgagcggg taaggcttgc aagctttcgc
    26461 gtttttcctg taagccgtgg cgggctaaaa atgcgtcatc ctggagtagc ttattaagtg
    26521 catcgcggta ttgagcgaat ttcggatggt cagatgcgtc catcatattg tcgatgaact
    26581 gctgcatcgg cggttgctta ataccttcga ggttgtaacc gacgctcata ttaaagatga
    26641 acgatttaca ggaatcagaa ggctggaaca tcgcttcgag aaggtgcagg gcaaaccagg
    26701 ctttgaggta ttcatcccag gctttaagca gggtaaattc ggtagaccat tcggtgttaa
    26761 agcactcgtc ttcggcatcg atacagggct tttccagctc caggcggtca agaatttgga
    26821 cggtttttag ttcgatgaag cgtccgccag tcagccagga agtgacaata ttttgcgcga
    26881 gctgcgtgtg cgggccagcg gcagggccga cgggagtggc acaggtttca ccgaagacgc
    26941 taacagtttt accttttacg ggtgagtaaa actgttgctc gggaatacca aagattgagc
    27001 gttgttgttg gtattcatca aatatgcgcg tcaaaagttc ctcaaacgga atgggacgca
    27061 taatatcccc cataaccctc tccttagctt atacagatat cattgagtga ccggtgttga
    27121 gaaatccggt ggtttagggg gatagagcaa caatcgcacc actcttgcca ggtttggcgg
    27181 gaaatataaa aaaatgtgag gcggctcgaa acttaatggc tttttgtgaa tgaactcaca
    27241 ggtgccaaat gagaattaaa atcactcctg aaaattatta ttgccagccc atttatcact
    27301 ttt
//
LOCUS       DAZFLD010000049        26842 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.049, whole genome shotgun sequence.
ACCESSION   DAZFLD010000049 DAZFLD010000000
VERSION     DAZFLD010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26842)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26842
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.049"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            355..570
                     /gene="essD"
                     /locus_tag="ACHKBL_001705"
     CDS             355..570
                     /gene="essD"
                     /locus_tag="ACHKBL_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage lysis protein EssD"
                     /protein_id="HFO5818585.1"
                     /translation="MKSMDKLTTGVAYGTSAGSAGYWFLQLLDKVTPSQWAAIGVLGS
                     LVFGLLTYLTNLYFKIKEDKRKAARGE"
     gene            570..1067
                     /gene="rrrD"
                     /locus_tag="ACHKBL_001706"
     CDS             570..1067
                     /gene="rrrD"
                     /locus_tag="ACHKBL_001706"
                     /EC_number="3.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme RrrD"
                     /protein_id="HFO5818586.1"
                     /translation="MPPSLRKAVAAAIGGGAIAIASVLITGPSGNDGLEGVSYIPYKD
                     IVGVWTVCHGHTGKDIMLGKTYTKAECKALLNKDLATVARQINPYIKVDIPETMRGAL
                     YSFVYNVGAGNFRTSTLLRKINQGDIKGACDQLRRWTYAGGKQWKGLMTRREIEREIC
                     LWGQQ"
     gene            1284..1466
                     /gene="rzoD"
                     /locus_tag="ACHKBL_001707"
     CDS             1284..1466
                     /gene="rzoD"
                     /locus_tag="ACHKBL_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage lysis lipoprotein RzoD"
                     /protein_id="HFO5818587.1"
                     /translation="MRKLKMMLCVMMLPLVVVGCTSKQSVSQCVKPPPPPAWIMQPPP
                     DWQTPLNGIISPSERG"
     gene            complement(1557..1850)
                     /gene="iss"
                     /locus_tag="ACHKBL_001708"
     CDS             complement(1557..1850)
                     /gene="iss"
                     /locus_tag="ACHKBL_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="increased serum survival lipoprotein Iss"
                     /protein_id="HFO5818588.1"
                     /translation="MKKMLFSAALAMLITGCAQQTFTVGNKPTAVTPKETITHHFFVS
                     GIGQEKTVDAAKICGGAENVVKTETQQTFVNGFLGFITLGIYTPLEARVYCSQ"
     gene            complement(2211..2405)
                     /gene="ylcI"
                     /locus_tag="ACHKBL_001709"
     CDS             complement(2211..2405)
                     /gene="ylcI"
                     /locus_tag="ACHKBL_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001307652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HFO5818589.1"
                     /translation="MSTKNRTRRTTTRNIRFPNQMIEQINIALEQKGSGNFSAWVIEA
                     CRRRLTSEKRAYTSIQSDDG"
     gene            2795..3340
                     /gene="nohD"
                     /locus_tag="ACHKBL_001710"
     CDS             2795..3340
                     /gene="nohD"
                     /locus_tag="ACHKBL_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HFO5818590.1"
                     /translation="MEVNKKQLADIFGASIRTIQNWQEQGMPVLRGGGKGNEVLYDSA
                     AVIKWYAERDAEIENEKLRREVEELRQASETDLQPGTIEYERHRLTRAQADAQELKNA
                     RDSAEVVETAFCTFVLSRIAGEIASILDGIPLSVQRRFPELENRHVDFLKRDIIKAMN
                     KAAALDELIPGLLSEYIEQSG"
     gene            3315..5240
                     /locus_tag="ACHKBL_001711"
     CDS             3315..5240
                     /locus_tag="ACHKBL_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HFO5818591.1"
                     /translation="MNISNSQVNRLRHFVRAGLRSLFRPEPQTAVEWADANYYLPKES
                     AYQEGRWETLPFQRAIMNAMGSDYIREVNVVKSARVGYSKMLLGVYAYFIEHKQRNTL
                     IWLPTDGDAENFMKTHVEPTIRDIPSLLALAPWYGKKHRDNTLTMKRFSNGRGFWCLG
                     GKAAKNYREKSVDVAGYDELAAFDEDIEQEGSPTFLGDKRIEGSVWPKSIRGSTPKVR
                     GTCQIERAASESPHFMRFHVACPHCGEEQYLKFGDKETPFGLKWTPDDPSSVFYLCEH
                     NACVIRQQELDFTDARYICEKTGIWTRDGILWFSSSGEEIEPPDSVTFHIWTAYSPFT
                     TWVQIVKDWMKTKGDTGKRKTFVNTTLGETWEAKIGERPDAEVMAERKEHYSAPVPDR
                     VAYLTAGIDSQLDRYEMRVWGWGPGEESWLIDRQIIMGRHDDEQTLLRVDEAINKTYT
                     RRNGAEMSVSRICWDTGGIDPTIVYERSKKHGLFRVIPIKGASVYGKPVASMPRKRNK
                     NGVYLTEIGTDTAKEQIYNRFTLTPEGDEPLPGAVHFPNNPDIFDLTEAQQLTAEEQV
                     EKWVDGRKKILWDSKKRRNEALDCFVYALAALRISISRWQLDLSALLASLQEEDGAAT
                     NKKTLADYARALSGEDE"
     gene            5237..5443
                     /locus_tag="ACHKBL_001712"
     CDS             5237..5443
                     /locus_tag="ACHKBL_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019058 - viral life cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail joining protein"
                     /protein_id="HFO5818592.1"
                     /translation="MTRQEELAAARAALHDLMTGKRVATVQKDGRRVEFTATSVSDLK
                     KYIAELEVQTGMTQRRRGPAGFYV"
     gene            5440..7041
                     /locus_tag="ACHKBL_001713"
     CDS             5440..7041
                     /locus_tag="ACHKBL_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO5818593.1"
                     /translation="MKTSSIPTLLGPDGMTSLREYAGYHGGGSGFGGQLRAWNPPSES
                     VDAALLPNFTRGNARADDLVRNNGYAANAIQLHQDHIVGSFFRLSHRPSWRYLGIGEE
                     EARAFSREVEAAWKEFAEDDCCCIDVERKRTFTMMIREGVAMHAFNGELFVQATWDTS
                     SSRLFRTQFRMVSPKRISNPNNTGDSRNCRAGVQINDSGAALGYYVSEDGYPGWMPQK
                     WTWIPRELPGGRASFIHVFEPVEDGQTRGANVFYSVMEQMKMLDTLQNTQLQSAIVKA
                     MYAATIESELDTQSAMDFILGANSQEQRERLTGWIGEIAAYYAAAPVRLGGAKVPHLM
                     PGDSLNLQTAQDTDNGYSVFEQSLLRYIAAGLGVSYEQLSRNYAQMSYSTARASANES
                     WAYFMGRRKFVASRQASQMFLCWLEEAIVRRVVTLPSKARFSFQEARSAWGNCDWIGS
                     GRMAIDGLKEVQEAVMLIEAGLSTYEKECAKRGDDYQEIFAQQVRETMERRAAGLKPP
                     AWAAAAFESGLRQSTEEEKSDSRAA"
     gene            7022..8341
                     /locus_tag="ACHKBL_001714"
     CDS             7022..8341
                     /locus_tag="ACHKBL_001714"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HFO5818594.1"
                     /translation="MTAELRNLPHIASMAFNEPLMLEPAYARVFFCALAGQLGISRLT
                     DAVSGDSLTAGEAPAALALSGDDDGPRQARSYQVMNGIAVLPVSGTLVSRTRALQPYS
                     GMTGYNGIIARLQQAASDPMVDGILLDMDTPGGMVAGAFDCADIIARVRDIKPVWALA
                     NDMNCSAGQLLASAASRRLVTQTARTGSIGVMMAHSNYGAALEKQGVEITLIYSGSHK
                     VDGNPYSHLPGDVRETLQSRMDATRRMFAQKVSAYTGLSVQAVLDTEAAVYSGQEAID
                     AGLADELVNSTDAITVMRDALDARKSRLSGGRMTKETQSTTVSATASQADVTDVVQAT
                     EGENASAAQPDVNAQITAAVAAENSRIMGILNCEEAHGREEQARVLAETPGMTVETAR
                     RILAAAPQSAQARSDTALDRLMQGAPAPLAAGNPASDAVNDLLNTPV"
     gene            8351..8683
                     /locus_tag="ACHKBL_001715"
     CDS             8351..8683
                     /locus_tag="ACHKBL_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HFO5818595.1"
                     /translation="MTSKETFTHYQPLGNSDPAHTATAPGGLSAKAPAMTPLMLDTST
                     RKLVAWDGTTDGTAVGILAVDADQTSTTLTFYKSGTFRYEDVLWPEAASDETKKRTAF
                     AGTAISIV"
     gene            8739..9764
                     /locus_tag="ACHKBL_001716"
     CDS             8739..9764
                     /locus_tag="ACHKBL_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFO5818596.1"
                     /translation="MSMYTTAQLLAANEQKFKFDPLFLRLFFRESYPFTTEKVYLSQI
                     PGLVNMALYVSPIVSGEVIRSRGGSTSEFTPGYVKPKHEVNPQMTLRRLPDEDPQNLA
                     DPAYRRRRIIMQNMRDEELAIAQVEEMQAVSAVLKGKYTMTGEAFDPVEVDMGRSAAN
                     NITQSGGTEWSKRDKSTYDPTDDIEAYALNASGVVNIIVFDPKGWALFRSFKAVKEKL
                     DTRRGSHSELETAVKDLGEAVSYKGMYGDTAIVVYSGQYVENDVKKNFLPDNTMVLGN
                     TQARGLRTYGCIQDADAQREGINASARYPKNWVTTGDPAREFTMIQSAPLMLLADPDA
                     FVSVQLA"
     gene            9806..10201
                     /locus_tag="ACHKBL_001717"
     CDS             9806..10201
                     /locus_tag="ACHKBL_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HFO5818597.1"
                     /translation="MTKDELIARLQVLGEQLNRDVSLTGTKEELVLRVAELEEELDDT
                     DDAAGQDTSVSPENALTGHENEVVSAQPDTVIDTAALVTVVALVTLHTDALHATRDEP
                     VAFVLPGTAFRVSAGVAAEMTEHGLARMQ"
     gene            10213..10566
                     /locus_tag="ACHKBL_001718"
     CDS             10213..10566
                     /locus_tag="ACHKBL_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HFO5818598.1"
                     /translation="MADSDNLFDAAIARADETIRGYMGTSATMTSGELSGAVIRGVFD
                     DPENISYAGQGVRVEGSSPSLFVRTDDVRQLRRGDTLTIGEENFWVDRISPDDGGSCH
                     LWLGRGVPPAVNRRR"
     gene            10578..11156
                     /locus_tag="ACHKBL_001719"
     CDS             10578..11156
                     /locus_tag="ACHKBL_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO5818599.1"
                     /translation="MAIKGLEQAVENLSRISKTAVPGAAAMAINRVASSAISQSASQV
                     ARETKVRRKLVKERARLKRATVKNPQARIRVNRGDLPVIKLGNARVVLSRRRRRKKGQ
                     RSSLKGGGSVLVVGNRRISGAFIQQLKNGRWHVMQRVAGKNRYPIDVVKIPMAVPLTT
                     AFKQNIERIRRERLPKELGYALQHQLRMVIKR"
     gene            11153..11548
                     /locus_tag="ACHKBL_001720"
     CDS             11153..11548
                     /locus_tag="ACHKBL_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail U family protein"
                     /protein_id="HFO5818600.1"
                     /translation="MKHTELRAAVLDALEKHDTGATFFDGRPAVFDEADFPAVAVYLT
                     GAEYTGEELDSDTWQAELHIEVFLPAQVPDSELDAWMESRIYPVMSDIPALSDLITSM
                     VASGYDYRRDDDAGLWSSADLTYVITYEM"
     gene            11556..12296
                     /locus_tag="ACHKBL_001721"
     CDS             11556..12296
                     /locus_tag="ACHKBL_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO5818601.1"
                     /translation="MPVPNPVMPVKGAGTTLWVYKGSGDPYANPLSDVDWSRLAKVKD
                     LTPGELTAESYDDSYLDDEDADWTATGQGQKSAGDTSFTLAWMPGEQGQQALLAWFNE
                     GDTRAYKIRFPNGTVDVFRGWVSSIGKAVTAKEVITRTVKVTNVGRPSMAEDRSTVTA
                     TTGMTVTPASTSVVKGQSTTLTVAFQPEGATDKSFRAVSADKTKATVSVSGMTITVKG
                     VAAGKVNIPVVSGNGELAAVAEITVTAS"
     gene            12312..12734
                     /locus_tag="ACHKBL_001722"
     CDS             12312..12734
                     /locus_tag="ACHKBL_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HFO5818602.1"
                     /translation="MFLKTESFEHNGVTVTLSELSALQRIEHLALMKRQAEQAESDSN
                     RKFTVEDAIRTGAFLVAMSLWHNHPQKTQMPSMNEAVKQIEQEVLTTWPTEAISHAEN
                     VVYRLSGMYEFVVNNAPEQTEDAGPAEPVSAGKCSTVS"
     gene            12716..13150
                     /locus_tag="ACHKBL_001723"
     CDS             12716..13150
                     /locus_tag="ACHKBL_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HFO5818603.1"
                     /translation="MFDGELSFALKLAREMGRPDWRAMLAGMSSTEYADWHRFYSTHY
                     FHDVLLDMHFSGLTYTVLSLFFSDPDMHPLDFSLLNRREADEEPEDDVLMQKAAGLAG
                     GVRFGPDGNEVIPASPDVADMTEDDVMLMTVSEGIAGGVRYG"
     gene            13143..15722
                     /locus_tag="ACHKBL_001724"
     CDS             13143..15722
                     /locus_tag="ACHKBL_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO5818604.1"
                     /translation="MAEPVGDLVVDLSLDAARFDEQMARVRRHFSGTETDAKKTAAVV
                     EQSLSRQALAAQKAGISVGQYKAAMRMLPAQFTDVATQLAGGQSPWLILLQQGGQVKD
                     SFGGMIPMFRGLAGAITLPMVGATSLAVATGALAYAWYQGNSTLSDFNKTLVLSGNQA
                     GLTADRMLVLSRAGQAAGLTFNQTSESLTALVNAGVRGGEQFEAISQSVARFSSASGV
                     EVDKVAEAFGKLTTDPTSGLTAMARQFHNVTAEQIAYVAQLQRSGDEAGALQAANEAA
                     TKGFDDQTRRLKENMGTLETWADRTARAFKSMWDSVLDIGRPDTAQGMLEKAEKAFDE
                     ADKKWQWYQSRSHRRGKTSAFLANLRGAWEDRANAQLGLSAATLQADLEKAREMAAKD
                     WAESEASRLKYTEEAQKAYERLQTPLEKYTARQEELNKALKDGKILQADYNTLMAAAK
                     KDYEATLKKPKQSGVKVSAGDRQEDSAHAALLTLQAELRTLEKHAGANEKISQQRRDL
                     WKAESQFAVLEEAAQRRQLSAQEKSLLAHKDETLEYKRQLAALGDKVTYQERLNALAQ
                     QADKFAQQQRAKRAAIDAKSRGLTDRQAEREATEQRLKEQYGDNPLALNNVMSEQKKT
                     WAAEDQLRGSWMAGLKSGWSEWEESATDSMSQVKSAATQTFDGIAQNMAAMLTGSEQN
                     WRSFTRSVLSMMTEILLKQAMVGIVGSIGSAIGGAVGGGASASGGTAIQAAAAKFHFA
                     TGGFTGTGGKYEPAGIVHRGEFVFTKEATSRIGVGNLYRLMRGYATGGYVGTPGSMAD
                     SRSQASGTFEQNNHVVINNDGTNGQIGPAALKAVYDMARKGARDEIQTQMRDGGLFSG
                     GGR"
     gene            15719..16048
                     /locus_tag="ACHKBL_001725"
     CDS             15719..16048
                     /locus_tag="ACHKBL_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO5818605.1"
                     /translation="MKTFRWKVKPGMDVASVPSVRKVRFGDGYSQRAPAGLNANLKTY
                     SVTLSVPREEATVLESFLEEHGGWKSFLWTPPYEWRQIKVTCAKWSSRVSMLRVEFSA
                     EFEQVVN"
     gene            16048..16746
                     /locus_tag="ACHKBL_001726"
     CDS             16048..16746
                     /locus_tag="ACHKBL_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFO5818606.1"
                     /translation="MQDIRQETLNECTRAEQSASVVLWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQPYPIQGSGFELNGKGTSTRPTLTVSNLYGMVTGMAEDMQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSDADPEQEVISRWRIEQCSELSAVSASFVLSTPTETDG
                     AVFPGRIMLANTCTWTYRGDECGYHGPAVADEYDQPTSDITKDKCSKCLSGCKFRNNV
                     GNFGGFLSINKLSQ"
     gene            16752..17495
                     /locus_tag="ACHKBL_001727"
     CDS             16752..17495
                     /locus_tag="ACHKBL_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFO5818607.1"
                     /translation="MTETESAILAHARRCAPAESCGFVVRTPEGERYFPCVNISGEPE
                     AYFRMSPEDWLRAEMQGEIVALVHSHPGGLPWLSETDRRLQVQSDLPWWLVCRGAIHK
                     FRCVPHLSGRRFEHGVTDCYTLFRDAYHLAGIEMPDFHREDDWWRHGQNLYLDNLEAT
                     GLYQVPLSSAQPGDVLLCCFGSSVPNHAAIYCGDSELLHHIPEQLSKRERYTDKWQRR
                     THSLWRHREWHASAFTGICNDLAAASTFV"
     gene            17492..18064
                     /locus_tag="ACHKBL_001728"
     CDS             17492..18064
                     /locus_tag="ACHKBL_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFO5818608.1"
                     /translation="MKTGAEAIRALATQLPAFRQKLSDGWYQVRIAGRDVSTSGLTAQ
                     LHETLPDGAVIHIVPRVAGAKSGGVFQIVLGAAAIAGSFFTAGATLAAWGAAIGAGGM
                     TGILFSLGASMVLGGVAQMLAPKARTPRTQTTDNGKQNTYFSSLDNMVAQGNVLPVLY
                     GEMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            18124..21621
                     /locus_tag="ACHKBL_001729"
     CDS             18124..21621
                     /locus_tag="ACHKBL_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HFO5818609.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPIEGPVDGLKS
                     VLLNSTPVLDSEGNTNISGVTVVFRAGEQEQTPPEGFESSGSETVLGTEVKYDTPITW
                     TITSANIDRLRVTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVGNLPPRPFSIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDSEQFGSQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTFKPAYSNNMAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQNCDQSVPDGFGGTEPRITCNAWLTTQRK
                     AWDVLSDFCSAMRCMPIWNGQTLTFVQDRPSDKVWTYNRSNVVMPDDGAPFRYSFSAL
                     KDRHNAVEVNWIDPDNGWETATELVEDTQAIARYGRNVTKMDAFGCTSRGQAHRAGLW
                     LIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGISTGGRVLAVNSQTRTLTLD
                     REITLPSSGTTLISLVDGSGNPVSVEVQSVTDGVKVKVSRVPDGVAGYSVWGLKLPTL
                     RQRLFRCVSIRENDDGTYAITAVQHVPEKEAIVDNGAHFDGDQSGTVNGVTPPAVQHL
                     TAEVTADSGEYQVLARWDTPKVVKGVSFLLRLTVTADDGSERLVSTARTTETTYRFTQ
                     LALGNYRLTVRAVNAWGQQGDPASVSFRIAAPAAPSRIELTPGYFQITATPHLAVYDP
                     TVQFEFWFSEKRIADIRQVETSARYLGTALYWIAASINIKPGHDYYFYVRSVNTVGKS
                     AFVEAVGQPSDDASGYLDFFKGEIGKTHLAQELWTQIDNGQLAPDLTEIRTSITDVSN
                     EITQTVNKKLEDQSAAIQQIQKVQVDTNNNLNSMWAVKLQQMQDGRLYIAGIGAGIEN
                     TPDGMQSQVLLAADRIAMINPANGNTKPMFVGQGDQIFMNEVFLKRLTAPTITSGGNP
                     PAFSLTSDGRLTAKNADISGSVNANSGTLNNVTINQNCTIKGMLEATQVRGDFVKAVS
                     KAFPKKVGTWGNTETPNGTVTVTISDDHNFDRQIIIPPIIFNGIAYDDPGSGNNPGGT
                     RYTGYGFEVRKNGVLIASRETKGAIPGSYSAVIDMPSGRGSVTLEFKIFQKGNQGAGN
                     ITDCTVIVTKKAASGISIR"
     gene            21692..22291
                     /locus_tag="ACHKBL_001730"
     CDS             21692..22291
                     /locus_tag="ACHKBL_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family protein"
                     /protein_id="HFO5818610.1"
                     /translation="MRKVCAAILSAAICLAVSGAPAWASEHQSTLSAGYLHASTNVPG
                     SDDLNGINVKYRYEFTDTLGLVTSFSYAGDKNRQLTRYSDTRWHEDSVRNRWFSVMAG
                     PSVRVNEWFSAYAMAGVAYSRVSTFSGDYLRVTDNKGKKHDVLTGSDDGRHSNTSLAW
                     GAGVQFNPTESVAIDIAYEGSGSGDWRTDGFIVGVGYKF"
     gene            22356..25382
                     /locus_tag="ACHKBL_001731"
     CDS             22356..25382
                     /locus_tag="ACHKBL_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HFO5818611.1"
                     /translation="MAVKISGVLKDGTGKPVQNCTIQLKAKRNSTTVVVNTLASENPD
                     EAGRYSMDVEYGQYSVILLVEGFPPSHAGTITVYEDSRPGTLNDFLGAMTEDDARPEA
                     LRRFELMVEEVARNASAVAQNTAAAKKSASDASTSAREAATHATDAADSARAASTSAG
                     QAASSAQSASSSAGTASTKATEASKSAAAAESSKSVAATSAGAAKTSETNAAASEQSA
                     ATSASTATTKASEAATSARDAAASKEAAKSSETNASLSASSAASSATAAGNSAKAAKT
                     SETNARSSETAAGQSASAAAGSKTAAASSASAASTSAGQASASATAAGKSAESAASSA
                     STATTKAGEATEQATAAARSASAAKTSETNAKASETRAESSKTAAASSASSAASSASS
                     ASASKDEATRQASAAKGSATTASTKATEAAGSAKAAAQSKSTAESAATRAETAAKRAE
                     DIASAVALEDASTTKKGIVQLSSATNSTSESLAATPKAVKAAYDLASGKYTAQDATTA
                     QKGIIQLSSATNSTSETLAATPKAVKAANDNAEKRLQKDQNGADIPGKDTFTKNIGAC
                     RAFGGSVSTTTGNWTTAQFIEWLDSQGAFNHPYWMCKGSWSYGNNKIITDTGCGNIHL
                     AGAVIEVMGIKSAMTIRITTPTTSSGGGTTNAQFTYINHGTDYSPGWRRDYNSRNKPT
                     ASEIGALPSGGTAVSSVNLASKGRVAALTDNTQGAAGLELYEVYNNGYPTTYGNIIHL
                     KGMTAVGEGELLIGWSGTSGAHAPAFIRSRRDTTDANWSPWAQLYTSAHPPAEFYPVG
                     APIPWPSDTVPSGYVLMQGQTFDKSAYPKLAAAYPSGVIPDMRGWTIKGKPASGRAVL
                     SQEQDGIKSHTHSASASSTDLGTKTTSSFDYGTKSTNNIGAHTHSVSGTAASAGNHTH
                     SVTGASAVSQWSQNGSVHKVVSAASVNTSAAGAHTHSVSGTAASAGAHAHTVGIGAHT
                     HSVAIGSHGHTITVNAAGNAENTVKNIAFNYIVRLA"
     gene            25379..26281
                     /locus_tag="ACHKBL_001732"
     CDS             25379..26281
                     /locus_tag="ACHKBL_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000631340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macro domain-containing protein"
                     /protein_id="HFO5818612.1"
                     /translation="MITLILSAPVTEMAEAFKRVFANADNVNIVGKPFETIREFDCMV
                     SAANSFGLMDGGVDAAITTFFGSQLQARVQNHILREYLGEQPVGSAFVIETGHNYHPW
                     LVHAPTMRVPLTIDGTDAVYNATWAALLAIFQHNKNATTDRKIKTVVFPAMGAGCGQV
                     PFESVARQMKQAWDNFNKKTESINWEYAQSRQSAVFGTYAYCPGNSVCRYADTKYIGC
                     GDYRTYCSRSGQVCINPEHQADDVLIQHQANNRFRPGSHIHRINPENPVGNVISGAHS
                     HGSSIVIGAHTHTLNKQYSVSDIK"
     gene            26290..>26842
                     /locus_tag="ACHKBL_001733"
     CDS             26290..>26842
                     /locus_tag="ACHKBL_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFO5818613.1"
                     /translation="MDFRMSEQPRTITIYNLLAGTNEFIGEGDAYIPPHTGLPANSTD
                     IAPPDIPAGFVAVFNSDESSWHLVEDHRGKTVYDVASGDALFISELGSLPENVTWLSP
                     EGEFQKWNGTAWVKDTEAEKLFRIREAEETKNSLMQVASEHIAPLQDAVDLEIATEKE
                     TLLLEAWKKYRVLLNRVDTSTAPD"
BASE COUNT         6405 a         6699 c         8078 g         5660 t
ORIGIN      
        1 taatttgaag gttcaattta aatcggctaa aaataacacc ggaaattatt tgttggttat
       61 ttgttgagat ttgcttatgt atttgtagtg gtgttttcaa tactcggtag cattctcgca
      121 aatatcattt tgtggtttac gtacgtaaaa aattggttat gctgttaaga gtggttactt
      181 cgtcacacag cttaaacccg ccgtcgagcg ggtttttcca ttttttgagt ctcgatatta
      241 gctgataacc caatacctga gttattcact gactccgagt ctgttacgtt tctgcttttt
      301 tgcgatacgt tgtattccct caatttacac ccgctttgtc tgcgaggtgg ggttatgaaa
      361 tccatggata agttaacaac gggtgtcgcc tatggcacct cagcaggtag tgccggttac
      421 tggtttttac agctgctcga taaagtcacg ccctcacagt gggcagcaat aggtgtgctg
      481 ggtagcctgg tatttggcct gctgacgtac ctgacaaacc tttatttcaa gattaaagaa
      541 gataagcgca aggctgcgag aggtgaataa tgcctccatc attacgaaaa gccgttgctg
      601 ctgctattgg tggcggagca attgctatag catcagtgtt aatcactggc ccaagtggta
      661 acgatggtct ggaaggtgtc agctacatac catacaaaga tattgttggt gtatggactg
      721 tatgtcacgg gcatacagga aaagacatca tgctcggtaa aacgtatacc aaagcagaat
      781 gcaaagccct cctgaataaa gaccttgcca cggtcgccag acaaattaac ccgtacatca
      841 aagtcgatat accggaaaca atgcgcggcg ctctttactc attcgtttac aacgtgggtg
      901 ctggcaattt cagaacatcg acgcttcttc gcaaaataaa ccagggcgat atcaaaggcg
      961 catgtgatca gctacgtcgc tggacatatg ctggcggtaa gcaatggaaa ggtctcatga
     1021 ctcgtcgtga gattgagcgt gaaatctgtt tgtggggtca gcaatgaaca gagtaaccgc
     1081 gattatctcc gctctggtta tctgcatcat cgtctgcctg tcatgggctg ttaatcatta
     1141 ccgtgataac gccattacct acaaagccca gcgcgacaaa aatgccagag aactgaagct
     1201 ggcgaacgcg gcaattactg acatgcagat gcgtcagcgt gatgttgctg cgctcgatgc
     1261 aaaatacacg aaggagttag ctgatgcgaa agctgaaaat gatgctctgc gtgatgatgt
     1321 tgccgctggt cgtcgtcggt tgcacatcaa agcagtctgt cagtcagtgc gtgaagccac
     1381 caccgcctcc ggcgtggata atgcagcctc cccccgactg gcagacaccg ctgaacggga
     1441 ttatttcacc ctcagagaga ggctgatcac tatgcaaaaa caactggaag gaacccagaa
     1501 gtatattaat gagcagtgca gatagagctg cccatatcga tgggcaactc atgcaattat
     1561 tgtgagcaat acacacgcgc ttccagcgga gtataaatgc ctaaagtaat aaaaccgaga
     1621 aatccattta cgaatgtttg ctgggtttct gttttaacaa cattttctgc gccgccacaa
     1681 attttggctg catcaacagt tttctcctgt ccaattcccg aaacgaagaa atgatgggtg
     1741 atggtttcct ttggtgttac tgctgtcggt ttgtttccaa cagtaaacgt ctgttgagca
     1801 catcctgtaa taagcattgc cagagcggca gaaaacaaca tttttttcat cttattatcc
     1861 tgcattgtta aaaacggcag aatcctatgt gacaacaatt aaacgatagt taaatggatt
     1921 gatgaaaatt aaaactatat aggtggatgc tcagcctatt ggaggagggg gggcactcag
     1981 aatcctgtgg aatgaaataa accgctctat ctgtccatta cccttttagc tgcgctgtat
     2041 cgtcgccgta ttcccgcatt aaccatgacc gtagcccgac ggggaattcc ttctgcgtga
     2101 gtgtgcggga ataatcaaaa acgatgcaca ccgggtttta ctgtgctgac agacgcaggg
     2161 ttaccctcat agtcgctttt ccggtgcgat ggtggaagaa accgggatgt tcatccatca
     2221 tcactttgga ttgatgtata tgctctcttt tctgacgtta gtctccgacg gcaggcttca
     2281 atgacccagg ctgagaaatt cccagaccct ttttgctcaa gagcgatgtt aatttgttca
     2341 atcatttggt taggaaagcg gatgttgcgg gttgttgttc tgcgggttct gttcttcgtt
     2401 gacatgaggt tgccccgtat tcagtgtcgc tgatttgtat tgtctgaagt tgtttttacg
     2461 ttaagttgat gcagatcaat taatacgata cctgcgtcat aattgattat ttgacgtggt
     2521 ttgatggcgt agatgcacgt tgtgacatgt agatgataat tattatcatt tttgcgggtc
     2581 ctttccggcg atccgacagg ttacggggcg gcgacctcgc gggttttcgc tatttatgaa
     2641 aattttccgg tttaaggcgt ttccgttctt cttcgtcata acttaatgtt tttatttaaa
     2701 ataccctctg aaaagaaagg aaacgacagg tgctgaaagc gagctttttg gcctctgtcg
     2761 tttcctttct ctgtttttgt ccgtggaatg aacaatggaa gtcaacaaaa agcagctggc
     2821 tgacattttc ggtgcgagta tccgtaccat tcagaactgg caggaacagg gaatgcccgt
     2881 tctgcgaggc ggtggcaagg gtaatgaggt gctttatgac tctgccgccg tcataaaatg
     2941 gtatgccgaa agggatgctg aaattgagaa cgaaaagctg cgccgggaag ttgaagaact
     3001 gcggcaggcc agcgagacag atctccagcc agggactatt gagtacgaac gccatcgact
     3061 tacgcgtgcg caggccgacg cacaggagct gaaaaatgcc agagactccg ctgaagtggt
     3121 ggaaaccgca ttctgtactt tcgtgctgtc gcggatcgca ggtgaaattg ccagtattct
     3181 cgacgggatc cccctgtcgg tgcagcggcg ttttccggaa ctggaaaacc gacatgttga
     3241 tttcctgaaa cgggatatca tcaaagccat gaacaaagca gccgcgctgg atgaactgat
     3301 accggggttg ctgagtgaat atatcgaaca gtcaggttaa caggctgcgg cattttgtcc
     3361 gcgccgggct tcgctcactg ttcaggccgg agccacagac cgccgttgaa tgggcggatg
     3421 ccaattacta tctcccaaaa gaatccgcat accaggaagg gcgctgggaa acactgccct
     3481 ttcagcgggc catcatgaat gcgatgggca gcgactacat ccgtgaggtg aatgtggtga
     3541 agtctgcccg tgtcggttat tccaaaatgc tgctgggtgt ttatgcctac tttatagagc
     3601 ataagcagcg caacaccctt atctggttgc cgacggatgg tgatgccgag aactttatga
     3661 aaacccacgt tgagccgacc atccgcgata ttccgtcgct gctggcgctg gctccgtggt
     3721 atggcaaaaa gcaccgggat aacacgctca ctatgaagcg tttttccaat ggtcgtggct
     3781 tctggtgcct gggcggtaaa gcggcaaaaa actaccgtga aaagtcggtg gatgtggcgg
     3841 gttatgatga acttgctgcc tttgatgagg atattgaaca ggaaggctct ccgacgttcc
     3901 ttggcgacaa acgtattgaa ggctcggtct ggccaaagtc catccgtggc tccacgccca
     3961 aagtgagagg cacctgccag attgagcgtg cagccagtga atccccgcat tttatgcgtt
     4021 ttcatgttgc ctgcccgcac tgcggggagg agcagtatct taaatttggc gacaaagaga
     4081 cgccgtttgg cctcaaatgg acgccggatg acccctccag cgtgttttat ctctgcgagc
     4141 ataatgcctg cgtcatccgc cagcaggagc tggactttac tgatgcccgt tatatctgcg
     4201 aaaagaccgg gatctggacc cgtgatggca ttctctggtt ttcgtcatcc ggtgaagaga
     4261 ttgagccgcc ggacagtgtg acctttcaca tctggacggc gtacagcccg ttcaccacct
     4321 gggtgcagat tgtcaaagac tggatgaaga cgaaagggga tacgggaaaa cgtaaaacct
     4381 tcgtgaacac cacgctcggt gagacatggg aagcgaaaat tggcgaacgt ccggatgctg
     4441 aggtgatggc ggagcggaaa gagcattatt cagcgcccgt tcctgaccgt gtggcttacc
     4501 tgaccgccgg tatcgactcc cagctggacc gctacgaaat gcgcgtatgg ggatgggggc
     4561 cgggtgagga aagctggctg attgaccggc agattattat gggccgccac gacgatgaac
     4621 agacgctgct gcgtgtggat gaggccatca ataaaaccta tacccgccgg aatggtgcag
     4681 aaatgtcggt atcccgtatc tgctgggata ctggcgggat tgacccgacc attgtgtatg
     4741 aacgctcgaa aaagcatggg ctgttccggg tgatccccat taaaggggca tccgtctacg
     4801 gaaagccagt ggccagcatg ccacgtaagc gaaacaaaaa cggggtttac cttaccgaaa
     4861 tcggtacgga taccgcgaaa gagcagattt ataaccgctt cacactgacg ccggaagggg
     4921 atgaaccgct tcccggtgcc gttcacttcc cgaataaccc ggatattttt gatctgaccg
     4981 aagcgcagca gctgactgct gaagagcagg tcgaaaaatg ggtggatggc aggaaaaaaa
     5041 tactgtggga cagcaaaaag cgacgcaatg aggcgctcga ctgcttcgtt tatgcgctgg
     5101 cggcgctgcg catcagtatt tcccgctggc agctggatct cagtgcactg ctggcgagcc
     5161 tgcaggaaga ggatggtgca gcaaccaaca agaaaacact ggcagattac gcccgtgcct
     5221 tatccggaga ggatgaatga cgcgacagga agaacttgcc gctgcccgtg cggcactgca
     5281 tgacctgatg acaggaaaac gggtggcaac ggtacagaaa gacggacggc gagtggagtt
     5341 tacggccact tccgtgtctg acctgaaaaa atacattgcg gagctggaag tgcagaccgg
     5401 catgacacag cgacgcaggg gacctgcagg attttatgta tgaaaacgtc ctccattccc
     5461 acccttctgg ggccggacgg catgacatcg ctgcgtgaat atgccggtta tcacggcggt
     5521 ggcagcggat ttggtgggca gttgcgggcg tggaacccac cgagtgaaag tgtggatgca
     5581 gccctgttgc ccaactttac ccgtggcaat gcccgcgcgg acgatctggt acgcaataac
     5641 ggctatgccg ccaacgccat ccagctgcat caggatcata tcgtcgggtc ttttttccgg
     5701 ctcagtcatc gcccaagctg gcgctatctg ggcatcgggg aggaagaagc ccgtgccttt
     5761 tcccgcgagg ttgaagcggc atggaaagag tttgccgagg atgactgctg ctgcattgac
     5821 gttgagcgaa aacgcacgtt taccatgatg attcgggaag gtgtggccat gcacgccttt
     5881 aacggtgaac tgttcgttca ggccacctgg gataccagtt cgtcgcggct tttccggaca
     5941 cagttccgga tggtcagccc gaagcgcatc agcaacccga acaataccgg cgacagccgg
     6001 aactgccgtg ccggtgtgca gattaatgac agcggtgcgg cgctgggata ttacgtcagc
     6061 gaggacgggt atcctggctg gatgccgcag aaatggacat ggataccccg tgagttaccc
     6121 ggcgggcgtg cctcgttcat tcacgttttt gaacccgtgg aggacgggca gacccgcggt
     6181 gcaaatgtgt tttacagcgt gatggagcag atgaagatgc tcgacacgct gcagaacacg
     6241 cagctgcaga gcgccattgt gaaggcgatg tatgccgcca ccattgagag tgagctggat
     6301 acgcagtcag cgatggattt tattctgggc gcgaacagtc aggagcagcg ggaaaggctg
     6361 accggctgga ttggtgaaat tgccgcgtat tacgccgcag caccggtccg tctgggaggc
     6421 gcaaaagtgc cgcacctgat gccgggggac tcactgaacc tgcagacggc tcaggacacg
     6481 gataacggct actccgtgtt tgaacagtca ctgttgcggt atatcgctgc cgggctgggt
     6541 gtctcgtatg agcagctttc ccggaattac gcccagatga gctactccac ggcacgggcc
     6601 agtgcgaacg agtcgtgggc gtactttatg gggcggcgaa aattcgtcgc atcccgtcag
     6661 gcgagccaga tgtttctgtg ctggctggaa gaggccatcg ttcgccgcgt ggtgacgtta
     6721 ccttcaaaag cgcgtttcag ctttcaggaa gcccgcagcg cctgggggaa ctgtgactgg
     6781 ataggctccg gtcgtatggc catcgatggt ctgaaagaag tacaggaagc ggtgatgctg
     6841 atagaagccg gactgagtac ctacgagaaa gagtgcgcaa aacgcggtga cgactatcag
     6901 gaaatttttg cccagcaggt ccgtgaaacg atggagcgcc gtgcagccgg tcttaaaccg
     6961 cccgcctggg cggctgcggc atttgaatcc ggactgcgac aatcaacaga ggaggagaag
     7021 agtgacagca gagctgcgta atctcccgca tattgccagc atggctttta atgagccgct
     7081 gatgcttgaa cccgcctatg cgcgggtttt cttttgtgcg cttgcaggcc agcttgggat
     7141 cagtcgcctg acggatgcag tatccggcga cagcctgact gccggagagg cacccgcggc
     7201 gctggcgtta tccggtgatg atgacggacc acgacaggcc cgcagttatc aggtcatgaa
     7261 cggcatcgcc gtgctgccgg tgtccggtac gctggtcagc cggacgcggg cgctgcagcc
     7321 gtattcggga atgaccggtt acaacggcat tatcgcccgt ctgcaacagg ctgccagcga
     7381 tccgatggtg gacggcattc tgctcgatat ggacacaccg ggcgggatgg tggcgggagc
     7441 atttgactgt gctgacatca tcgcccgtgt gcgtgacata aagccggtat gggcgctggc
     7501 caacgacatg aactgcagtg caggtcagct gcttgccagc gccgcctccc ggcgtctggt
     7561 cacgcagacc gcccggacag gctccatcgg cgtcatgatg gctcacagta attacggcgc
     7621 tgcgctggag aaacagggcg tggaaatcac gctgatttac agcggcagcc ataaggtgga
     7681 tggcaacccc tacagccatc taccgggtga tgtccgggag acactgcagt cccggatgga
     7741 tgcaacccgc cggatgtttg cgcagaaggt gtcggcatat accggcctgt ccgtgcaggc
     7801 tgtgctggat accgaggctg cagtgtacag cggtcaggag gccattgatg ccggactggc
     7861 tgatgaactt gtcaacagca ccgatgcgat caccgttatg cgtgatgcac tggatgcacg
     7921 taaatcccgt ctctcaggag ggcgaatgac caaagagact caatcaacaa ctgtttcagc
     7981 cactgcttcg caggctgacg ttactgacgt ggtgcaagcg acggagggcg aaaacgccag
     8041 cgcggcgcag ccggacgtga acgcgcagat caccgctgcg gttgcggcag aaaacagccg
     8101 cattatgggg atcctcaact gtgaggaagc tcacggacgc gaagaacagg cacgtgtgct
     8161 ggccgaaacc cccggtatga ccgtggaaac ggcccgccgc attctggcag ctgcaccaca
     8221 gagtgcacag gcgcgcagtg acactgcgct ggatcgtctg atgcaggggg caccggcacc
     8281 actggctgca ggtaacccgg catctgatgc cgttaacgat ttgctgaaca caccagtgta
     8341 agggatgttt atgacgagca aagaaacctt tacccattac cagccgctgg gcaacagtga
     8401 cccggcacat acggcaaccg cgcccggcgg attgagtgcg aaagcgcctg caatgacccc
     8461 gctgatgctg gacacctcca cccgtaagct ggttgcgtgg gatggcacca ccgacggtac
     8521 tgccgttggc attctggcgg ttgatgctga ccagaccagc accacgctga cgttctacaa
     8581 gtccggcacg ttccgttatg aggatgtgct ctggccggag gctgccagcg acgagacgaa
     8641 aaaacggacc gcgtttgccg gaacggcaat cagcatcgtt taaccttacc cttcatcact
     8701 aaaggccgcc tgtgcggctt tttttacggg atttttttat gtcgatgtac acaaccgccc
     8761 agctgctggc ggcaaatgag cagaaattta agtttgatcc gctgtttctg cgtctctttt
     8821 tccgtgagag ctatcccttc accacggaga aagtctatct ctcacaaatt ccgggactgg
     8881 taaacatggc gctgtacgtt tcgccgattg tttccggtga ggttatccgt tcccgtggcg
     8941 gctccacctc tgaatttacg ccgggatatg tcaaacccaa gcatgaggtg aatccgcaga
     9001 tgaccctgcg tcgcctgccg gatgaagatc cgcagaatct ggcggacccg gcttaccgcc
     9061 gccgtcgcat catcatgcag aacatgcgag acgaagagct ggccattgct caggtcgaag
     9121 agatgcaggc agtttctgct gtgcttaagg gcaaatacac catgaccggt gaagccttcg
     9181 atccggttga ggtggatatg ggccgcagtg cggcgaacaa catcacacag tccggtggta
     9241 cggagtggag caagcgtgac aagtccacgt atgacccgac cgacgatatc gaagcctatg
     9301 cgctgaacgc cagcggcgtg gtgaatatca tcgtgtttga tccgaaaggc tgggcgctgt
     9361 tccgttcctt caaagccgtc aaggagaagc tggatacccg tcgcggctct cattccgagc
     9421 tggagacagc ggtaaaagac ctgggcgaag cggtgtccta taaggggatg tatggcgata
     9481 cggcgatcgt cgtgtattcc ggacagtacg tggaaaacga cgtcaaaaag aacttcctgc
     9541 cggacaacac gatggtgctg gggaacactc aggcacgcgg tctgcgcacc tatggctgca
     9601 ttcaggatgc ggacgcacag cgcgaaggta ttaacgcctc tgcccgctac ccgaaaaact
     9661 gggtgaccac cggcgatccg gcgcgtgagt tcaccatgat tcagtcagca ccgctgatgc
     9721 tgctggctga tcctgatgcg ttcgtgtccg tacaactggc gtaatcatgg cccttcgggg
     9781 ccattttctc tctgtggagg agtccatgac gaaagatgaa ctgattgccc gtcttcaggt
     9841 gctgggtgag caactgaacc gtgatgtcag cctgacgggg acgaaagaag aactggtgct
     9901 ccgtgtggca gagctggaag aggagcttga tgacacggat gacgctgccg gtcaggacac
     9961 atctgtcagc ccggaaaatg cgctgaccgg acatgaaaat gaggtggtat cagcgcagcc
    10021 ggataccgtg attgatacgg ctgctctggt cacggtcgtg gcactggtga cgctgcatac
    10081 tgatgcactt cacgccacgc gggatgagcc tgtggcattt gtgctgccgg gaacggcgtt
    10141 tcgtgtctct gccggtgtgg cagccgaaat gacagaacat ggcctggcca gaatgcaata
    10201 acgggaggcg ctgtggctga ttccgataac ctgttcgatg ctgccattgc ccgcgccgat
    10261 gaaacgatac gcgggtacat gggaacgtca gccaccatga catccggtga gctgtccggt
    10321 gctgtgatac gtggtgtttt tgatgaccct gaaaatatca gctatgccgg acagggggtg
    10381 cgcgttgaag gctccagccc gtccctgttt gtccggactg atgatgtgcg gcagctgcgg
    10441 cgtggagaca cactgaccat cggcgaggaa aacttctggg tggaccggat ttcgccggat
    10501 gatggcggaa gctgtcatct ctggcttggg cgtggcgtgc cgcctgccgt taaccgtcgc
    10561 cgctgaaagg gggatgtatg gccataaaag gtcttgagca ggccgttgaa aacctcagcc
    10621 gtatcagcaa aacggcggtg cctggtgccg ccgcaatggc cattaaccgc gttgcgtcat
    10681 ccgcgatatc gcagtctgcg tcacaggttg cccgtgagac aaaggtacgc cggaaactgg
    10741 taaaggaaag ggccaggctg aaaagggcca cggtcaaaaa tccgcaggcc agaatcaggg
    10801 ttaaccgggg ggatttgccc gtaatcaagc tgggtaacgc gcgggttgtc ctttcccgcc
    10861 gcagacgtcg taaaaagggg cagcgttcat ccctgaaagg tggcggcagc gtgcttgtgg
    10921 tgggaaaccg tcgtatttcc ggcgcgttta ttcagcaact gaaaaatggc cggtggcatg
    10981 tcatgcagcg tgtggccggg aaaaaccgtt accccattga tgtggtgaaa atcccgatgg
    11041 cggtgccgct gaccacggcg tttaaacaga atattgaacg gatacggcgt gaacgtcttc
    11101 cgaaagagct gggctatgcg ctgcagcatc aactgagaat ggtaataaag cgatgaaaca
    11161 tactgaactc cgtgcagccg tactggatgc actggagaag catgacaccg gggcgacgtt
    11221 ttttgatggt cgccccgctg tttttgatga ggcggatttt ccggcagttg ccgtttatct
    11281 caccggcgct gaatacacgg gcgaagagct ggacagcgat acatggcagg cggagctgca
    11341 tattgaagtt ttcctgcctg ctcaggtgcc ggattcagag ctggatgcgt ggatggagtc
    11401 ccggatttat ccggtgatga gtgatatccc ggcactgtca gatttgatca ccagtatggt
    11461 ggccagcggc tatgactacc ggcgcgacga tgatgcgggc ctgtggagtt cagccgatct
    11521 gacttatgtc attacctatg aaatgtgagg acgctatgcc tgtaccaaat cctgtaatgc
    11581 cggtgaaagg tgccgggacc accctgtggg tttataaggg aagcggtgac ccttatgcga
    11641 atccgctttc agacgttgac tggtcgcgtc tggcaaaagt taaagacctg acgcccggcg
    11701 aactgaccgc tgagtcctat gacgacagct atctcgatga tgaagatgca gactggactg
    11761 cgaccgggca ggggcagaaa tctgccggag ataccagctt cacgctggcg tggatgcccg
    11821 gagagcaggg gcagcaggcg ctgctggcgt ggtttaatga aggggatacc cgagcctata
    11881 aaatccgctt cccgaacggc acggtcgatg tgttccgcgg ctgggtcagc agtatcggta
    11941 aggcggtgac ggcgaaggaa gtgatcaccc gcacggtgaa agtcaccaac gtgggccgtc
    12001 cgtcgatggc agaagatcgc agcacggtga cggcgacaac cggcatgact gtgacgcctg
    12061 ccagcacctc ggtggtgaaa gggcagagca ccacgctgac cgtggcattc cagccggaag
    12121 gcgcaaccga caagagcttc cgtgcggtgt ctgcggataa aacaaaagcc accgtgtcgg
    12181 tcagtggtat gaccatcacc gtgaaaggtg ttgctgcagg caaggtcaac attccggtcg
    12241 tatccggtaa tggtgaactt gctgcggttg cagaaatcac cgtcaccgcc agttaatccg
    12301 gggagtcagc gatgttcctg aaaaccgaat catttgaaca taacggtgtg accgtcacgc
    12361 tttctgaact gtcagccctg cagcgaattg agcatctcgc cctgatgaaa cggcaggcag
    12421 aacaggcgga gtcagacagc aaccggaagt ttactgtgga agacgccatc agaaccggcg
    12481 cgtttctggt ggcgatgtcc ctgtggcata accatccgca gaagacgcag atgccgtcca
    12541 tgaatgaagc cgttaaacag attgagcagg aagtgcttac cacctggccc acggaggcaa
    12601 tttctcatgc tgaaaacgtg gtgtaccggc tgtctggtat gtatgagttt gttgtgaata
    12661 atgcccctga acagacagag gacgccgggc ccgcagagcc tgtttctgcg ggaaagtgtt
    12721 cgacggtgag ctgagttttg ccctgaaact ggcgcgagag atggggcgac ccgactggcg
    12781 tgccatgctt gccgggatgt catccacgga gtatgccgac tggcaccgct tttacagtac
    12841 ccattatttt catgatgttc tgctggatat gcacttttcc gggctgacgt acaccgtgct
    12901 cagcctgttt ttcagcgatc cggatatgca tccgctggat ttcagtctgc tgaaccggcg
    12961 tgaggctgac gaagagcctg aagatgatgt gctgatgcag aaagcggcag ggcttgccgg
    13021 aggcgtccgc tttggcccgg acgggaatga agttatcccc gcttccccgg atgtggcgga
    13081 catgacggag gatgacgtaa tgctgatgac agtatcagaa gggatcgcag gaggagtccg
    13141 gtatggctga accggtaggc gatctggtcg ttgatttaag tctggatgcg gccagatttg
    13201 acgagcagat ggccagagtc aggcgtcatt tttccggtac ggaaactgat gcgaaaaaaa
    13261 cagcggcagt cgttgaacag tcgctgagcc gacaggcgct ggctgcacag aaagcgggga
    13321 tttccgtcgg gcagtataaa gccgccatgc gtatgctgcc tgcacagttc accgacgtgg
    13381 ccacgcagct tgcaggcggg caaagcccgt ggctgatcct gctgcaacag gggggtcagg
    13441 ttaaggactc cttcggcggg atgatcccca tgttcagggg gcttgccggt gcgatcaccc
    13501 tgccgatggt gggggccacc tcgctggcgg tggcgaccgg tgcgctggcg tatgcctggt
    13561 atcagggcaa ctcaaccctg tccgatttca acaaaacgct ggtcctttcc ggcaatcagg
    13621 cgggactgac ggcagatcgt atgctggtcc tgtccagagc cgggcaggcg gcagggctga
    13681 cgtttaacca gaccagcgag tcactgacgg cgctggtgaa tgccggtgtg cgtggtggtg
    13741 agcagtttga ggcgatcagc cagagtgtgg cgcgtttctc ctctgcatcc ggcgtggagg
    13801 tggacaaggt cgctgaagcc ttcgggaagc tgaccacaga cccgacgtcg ggactgacag
    13861 cgatggcacg tcagttccat aacgtgacgg cggagcagat tgcgtatgtt gctcagttgc
    13921 agcgttccgg agatgaagcc ggggcattgc aggcggcgaa cgaggccgca acgaaagggt
    13981 ttgatgacca gacccgccgc ctgaaagaga acatgggcac gctggagacc tgggcagaca
    14041 ggacagcacg ggcattcaaa tccatgtggg attcggtgct ggatattggt cgcccggaca
    14101 ctgcccaggg aatgctggag aaagcagaaa aggcttttga tgaggcggac aaaaaatggc
    14161 agtggtatca gagccggagc caccggcgcg gtaaaacctc agcatttctt gccaatctcc
    14221 ggggagcatg ggaggacaga gcgaatgcgc aacttgggct ttcagccgcc acgttgcagg
    14281 ccgatcttga aaaggccaga gagatggcag caaaggactg ggccgagtct gaggcatcac
    14341 ggctgaaata taccgaagag gcgcagaagg cttacgaacg gctgcagacg ccgctggaga
    14401 aatataccgc ccgtcaggaa gaactgaaca aggcactgaa agacgggaaa atcctgcagg
    14461 cggattacaa cacgctgatg gcggcggcga aaaaggatta tgaagcgacg ctgaaaaagc
    14521 cgaaacagtc cggcgtgaag gtgtctgcgg gcgatcgtca ggaagacagt gctcatgctg
    14581 ccctgctgac gcttcaggct gaactccgga cgctggagaa gcatgccgga gcaaatgaga
    14641 aaatcagcca gcagcgccgg gatttgtgga aggcggagag ccagttcgcg gtactggagg
    14701 aggcggcgca acgtcgccag ctgtctgcac aggagaaatc cctgctggcg cataaagatg
    14761 agacgctgga gtacaaacgc cagctggctg cacttggcga caaggttacg tatcaggagc
    14821 gcctgaacgc gctggcgcag caggcggata aattcgcaca gcagcaacgg gcaaaacggg
    14881 ccgccattga tgcgaaaagc cgggggctga ctgaccggca ggcagaacgg gaagccacgg
    14941 aacagcgcct gaaggaacag tatggcgata atccgctggc gctgaataac gtcatgtcag
    15001 agcagaaaaa gacctgggcg gctgaagacc agcttcgcgg gagctggatg gcaggcctga
    15061 agtccggctg gagtgagtgg gaagagagcg ccacggacag tatgtcgcag gtaaaaagtg
    15121 cagccacgca gacctttgat ggtattgcac agaatatggc ggcgatgctg accggcagtg
    15181 agcagaactg gcgcagcttc acccgttccg tgctgtccat gatgacagaa attctgctta
    15241 agcaggcaat ggtggggatt gtcgggagta tcggcagcgc cattggcggg gctgttggtg
    15301 gcggcgcatc cgcgtcaggc ggtacagcca ttcaggccgc tgcggcgaaa ttccattttg
    15361 caaccggagg atttacggga accggcggca aatatgagcc agcggggatt gttcaccgtg
    15421 gtgaatttgt cttcacgaag gaggcaacca gccggattgg cgtggggaat ctttaccggc
    15481 tgatgcgcgg ctatgccacc ggcggttatg tcggtacacc gggcagcatg gcagacagcc
    15541 ggtcgcaggc gtccgggacg tttgagcaga ataaccatgt ggtgattaac aacgacggca
    15601 cgaacggtca gataggtccg gctgctctga aggcggtgta tgacatggcc cgcaagggtg
    15661 cccgtgatga aattcagaca cagatgcgtg atggtggcct gttctccgga ggtggacgat
    15721 gaagaccttc cgctggaaag tgaaacccgg tatggatgtg gcttcggtcc cttctgtaag
    15781 aaaggtgcgc tttggtgatg gctattctca gcgagcgcct gccgggctga atgccaacct
    15841 gaaaacgtac agcgtgacgc tttctgtccc ccgtgaggag gccacggtac tggagtcgtt
    15901 tctggaagag cacgggggct ggaaatcctt tctgtggacg ccgccttatg agtggcggca
    15961 gataaaggtg acctgcgcaa aatggtcgtc gcgggtcagt atgttgcgtg ttgagttcag
    16021 cgcagagttt gaacaggtgg tgaactgatg caggatatcc ggcaggaaac actgaatgaa
    16081 tgcacccgtg cggagcagtc ggccagcgtg gtgctctggg aaatcgacct gacagaggtc
    16141 ggtggagaac gttatttttt ctgtaatgag cagaacgaaa aaggtgagcc ggtcacctgg
    16201 caggggcgac agtatcagcc gtatcccatt caggggagcg gttttgaact gaatggcaaa
    16261 ggcaccagta cgcgccccac gctgacggtt tctaacctgt acggtatggt caccgggatg
    16321 gcggaagata tgcagagtct ggtcggcgga acggtggtcc ggcgtaaggt ttacgcccgt
    16381 tttctggatg cggtgaactt cgtcaacgga aacagtgacg ccgatccgga gcaggaggtg
    16441 atcagccgct ggcgcatcga gcagtgcagc gaactgagcg cggtcagtgc ctcttttgta
    16501 ctgtccacgc cgacggaaac ggacggtgcc gtttttccgg gacgtatcat gctggccaac
    16561 acctgcacct ggacctatcg cggtgatgag tgcggttatc acggtccggc ggtcgcggat
    16621 gaatatgatc agccgacgtc cgatatcacg aaggataaat gcagcaaatg cctgagcggc
    16681 tgtaagtttc gcaataacgt cggcaacttt ggcggcttcc tttccattaa caaactttcg
    16741 cagtgaatcc catgacagag acagaatcag cgattctggc gcacgcccgg cgatgtgcgc
    16801 cagcggagtc gtgcggcttc gtggtgagaa caccggaggg ggaaagatat tttccctgcg
    16861 tgaatatctc cggtgagccg gaggcgtatt tccggatgtc gccggaggac tggctgcggg
    16921 cagagatgca gggtgagatt gtggcgctgg tccacagcca ccccggtggt ctgccctggc
    16981 tgagtgagac cgaccggcgg ctgcaggtgc agagtgattt gccgtggtgg ctggtttgcc
    17041 ggggggcgat tcacaagttc cgctgtgtgc cacatctttc cgggcggcgc tttgagcacg
    17101 gggtgacgga ctgttacacg ctgttccggg atgcttacca tctggcgggg attgagatgc
    17161 cggattttca tcgcgaggat gactggtggc gtcacggtca gaatctctat ctggataatc
    17221 tggaggccac agggctgtat caggtgccgt tgtcatcagc acaaccgggc gatgtgctgc
    17281 tgtgctgttt tggttcatcg gtgccgaatc atgccgccat ttactgtggt gacagcgagc
    17341 tgctgcacca tattcctgaa caactgagca aacgagagag gtacaccgac aaatggcagc
    17401 gacgcacaca ctccctctgg cgtcaccggg aatggcacgc atctgccttt acggggattt
    17461 gcaacgattt ggccgccgca tcgaccttcg tgtgaaaacg ggggctgaag ccatccgcgc
    17521 actggccaca cagctcccgg cgtttcgtca gaaactgagc gacggctggt atcaggtacg
    17581 gattgccggg cgggacgtca gcacgtccgg gttaacggcg cagttacatg agactctgcc
    17641 tgacggcgct gtgattcata ttgttcccag agtcgccggg gccaagtcag ggggcgtatt
    17701 ccagattgtt ctgggggctg ccgccattgc cggatcattc tttactgccg gagccaccct
    17761 tgcagcatgg ggggcagcca ttggggccgg tggtatgacc ggcatcctgt tttctctcgg
    17821 tgccagtatg gtgctcggtg gtgtggcgca gatgctggca ccgaaagcca gaactccccg
    17881 tacacagaca acggataacg gtaagcagaa cacctatttc tcctcactgg ataacatggt
    17941 tgcccagggc aatgttctgc ctgttctgta cggggaaatg cgcgtggggt ctcgcgtggt
    18001 ttctcaggag atcagcacgg cagacgaagg ggatggtggt caggttgtgg tgattggtcg
    18061 ctgatgaaaa acgtttatgt gaaaccgcct ccgggcggtt ttgtcgttta tggagcgtga
    18121 ggaatgggta aaggcagcag taaggggcat actccgcgcg aagcgaagga caacctgaag
    18181 tccacgcagt tgctgagtgt gatcgatgcc atcagcgaag ggccgattga aggtccggtg
    18241 gatggattaa aaagcgtgct gctgaacagt acgccagtgc tggacagtga ggggaatact
    18301 aacatctccg gcgtcacggt ggtgttccgg gccggtgagc aggagcagac accgccggag
    18361 ggatttgaat cctccggctc cgagacggtg ctgggtacgg aagtgaaata cgacacgccg
    18421 atcacctgga ccatcacgtc ggcaaacatt gaccgactgc gtgttacctt cggcgtgcag
    18481 gcactggtgg aaaccacctc aaagggggac aggaatccgt cggaagtccg cctgctggtt
    18541 cagatacagc gtaatggtgg ctgggtgacg gaaaaagaca tcaccattaa gggcaaaacc
    18601 acctcgcagt atctggcctc ggtggtggtg ggtaacctgc cgccgcgccc gttcagtata
    18661 cggatgcgca ggatgacgcc ggacagcacc acagaccagc tgcagaacaa aacgctctgg
    18721 tcgtcataca ccgaaatcat cgatgtgaaa cagtgctacc cgaacacggc actggtcggt
    18781 gtgcaggtgg actcggagca gttcggcagc cagcaggtga gccgtaatta tcatctgcgc
    18841 gggcgtattc tgcaggtgcc gtcgaactat aacccgcaga cgcggcaata cagcggtatc
    18901 tgggacggaa cgtttaaacc ggcatacagc aacaacatgg cctggtgtct gtgggatatg
    18961 ctgacccatc cgcgctacgg catggggaaa cgtcttggtg cggcggatgt ggataaatgg
    19021 gcgctgtatg tcatcggcca gaattgcgac cagtcggtgc cggacggctt tggcggcacg
    19081 gagccgcgca tcacctgtaa tgcctggctg accacacagc gcaaggcgtg ggatgtgctc
    19141 agtgatttct gctcggcgat gcgctgtatg ccgatatgga acgggcagac gctgacgttc
    19201 gtgcaggacc gaccatcaga taaggtgtgg acctataacc gcagtaatgt ggtgatgccg
    19261 gatgatggcg cgccgttccg ctacagcttc agcgccctga aggaccgcca taatgccgtt
    19321 gaggtgaact ggattgaccc ggataacggc tgggagacgg cgacagagct tgtggaggac
    19381 acgcaggcca ttgcccgtta cggtcgtaac gtcacgaaga tggatgcctt tggctgtacc
    19441 agtcgggggc aggcgcaccg cgccgggctg tggctgatta aaacggaact gctggaaacg
    19501 cagaccgtgg acttcagcgt gggtgcggaa gggcttcgcc atgtaccggg ggatgtcatt
    19561 gaaatctgcg atgatgacta tgcgggtatc agcaccggcg ggcgcgtgct ggcggtgaac
    19621 agccagaccc ggacgctgac gctcgaccgt gaaatcacgc tgccatcctc cggtaccacg
    19681 ctgataagcc tggttgacgg aagtggcaat ccggtcagcg tggaggtcca gtccgtcacc
    19741 gacggcgtga aggtaaaagt gagccgtgtt cctgacggcg ttgccggata cagcgtgtgg
    19801 gggctgaagc tgccgacgct gcgccagcga ctgttccgct gcgtgagtat ccgtgagaac
    19861 gacgacggca cgtatgccat caccgccgtg cagcatgtgc cggaaaaaga ggccatcgtg
    19921 gataacgggg cgcactttga cggcgaccag agcggcacgg tgaatggtgt cacgccgcca
    19981 gcggtgcagc acctgactgc cgaagtcacc gcagacagcg gggaatatca ggtgctggcg
    20041 cgctgggaca caccgaaggt ggtgaagggc gtgagcttcc tgctccgtct gaccgtaaca
    20101 gcggacgacg gcagtgagcg gctggtcagc acggcccgga cgacggaaac cacataccgc
    20161 ttcacacaac tggcgttggg gaactacagg ctgacagtcc gggcggtaaa tgcgtgggga
    20221 cagcagggcg atccggcatc ggtatcgttc cggattgccg caccggcagc gccgtctcgg
    20281 attgagctga caccgggcta ttttcagata accgccacgc cgcatcttgc ggtttatgac
    20341 ccgacggtac agtttgagtt ctggttctcg gaaaaacgga ttgcggatat caggcaggtt
    20401 gaaaccagcg cgcgttatct tggtacggca ctgtactgga tagccgccag tatcaatatc
    20461 aaaccgggcc atgattatta tttttacgtt cgcagtgtga acaccgttgg caaatcggca
    20521 ttcgtggagg ctgtcggtca gccgagtgat gatgcatcag gctatctgga ttttttcaaa
    20581 ggcgagatag ggaaaaccca tctggctcag gagctgtgga cgcagattga taacggtcag
    20641 cttgcgcctg acctgactga aatcaggacg tccataacgg atgtcagcaa tgaaataaca
    20701 cagaccgtca ataagaaact ggaagaccag agtgcagcga tccagcagat acagaaggtt
    20761 caggttgata caaataataa cctgaacagc atgtgggcag tgaagctgca gcagatgcag
    20821 gacggacgcc tttatattgc gggtatcggt gccggtattg agaacacccc tgacggcatg
    20881 cagagtcagg tgctgctggc agcagacagg attgcgatga ttaatcctgc gaatggcaac
    20941 acaaagccga tgtttgttgg tcagggcgat cagatattca tgaatgaagt gttcctgaaa
    21001 cgcctgacgg cccccaccat taccagcggc ggtaatcctc cggcattttc cctgacatca
    21061 gacgggagac tgacggcgaa aaatgcggat atcagtggca gtgtgaatgc gaactcagga
    21121 acgctcaaca atgtcacgat taaccagaac tgtacgatta agggcatgct ggaggcgacc
    21181 caggtcagag gagatttcgt taaagctgta tcaaaagcct tcccgaaaaa agtcggtacg
    21241 tggggtaaca cggaaacacc aaacggtacg gttacagtca ccatcagcga tgatcataac
    21301 tttgaccgcc agattattat tccgcccatt atttttaacg gtatagcgta tgacgatccg
    21361 gggagcggaa ataacccagg aggcacgcga tacacgggtt atggttttga agttcgcaaa
    21421 aacggcgtat taatcgcatc cagagaaact aaaggggcca ttcccggtag ttacagtgca
    21481 gttattgata tgccgagtgg caggggaagc gtcactctgg agtttaagat tttccagaaa
    21541 ggcaatcagg gggcaggcaa tatcaccgac tgtacggtga ttgtgaccaa aaaagccgct
    21601 tccggcatca gtattcgttg aaatatttat aaccccaata aagggcgtca ggaatgacgc
    21661 cttttttatt gcagaaaagc gagaggtaat tatgcgtaaa gtttgtgcag caattttgtc
    21721 cgcagccatt tgtctggccg tatccggtgc gcctgcatgg gcgtctgaac atcagtccac
    21781 gctgagcgcg gggtatcttc atgcctcgac gaacgtcccc ggcagcgatg atctgaacgg
    21841 gattaacgtg aaataccgtt atgagtttac ggacacactg gggctggtga cgtcattcag
    21901 ctatgcagga gacaagaatc gccagctgac ccgttacagc gatacccgct ggcatgaaga
    21961 ttccgttcgt aaccgctggt tcagcgtaat ggcggggccg tctgtgcgcg tgaatgaatg
    22021 gttcagcgcg tatgcgatgg cgggtgtagc ttacagccgt gtgtcgactt tctccgggga
    22081 ttatcttcgc gtaactgaca acaaggggaa aaagcacgat gtgctgaccg gaagtgatga
    22141 cggtcgccac agcaacacgt ctctggcgtg gggggctggc gtgcagttta acccgaccga
    22201 atccgtggcc attgatattg cttatgaagg ctccggcagt ggtgactggc gcactgacgg
    22261 tttcatcgtg ggtgtcggtt ataaattctg attagccagg taacacagtg ttatgacagc
    22321 ccgccggttc aggcgggctt ttttgtgggg tgaatatggc agtaaagatt tcaggtgtac
    22381 tgaaagacgg cacaggaaaa ccggtacaga actgcacaat ccagctgaaa gcaaaacgta
    22441 acagcaccac ggtggtggtg aacacgctgg cctcagaaaa tccggatgaa gccgggcgtt
    22501 acagcatgga cgttgagtac gggcagtaca gcgttattct gttggtggag ggattcccgc
    22561 cgtcacatgc cgggaccatc accgtgtatg aagattcccg acccggtacg ctgaatgatt
    22621 ttctcggtgc catgacggag gatgatgccc gtcctgaggc actgcgccgt tttgaactga
    22681 tggtggaaga ggtggcgcgt aacgcgtccg cggtggcaca gaacacggca gccgcgaaga
    22741 agtcagccag cgatgccagc acatcagccc gtgaggcggc aacccatgcg actgatgctg
    22801 cagattcagc acgtgcagcc agcacgtcag ccggacaggc cgcttcgtcg gctcagtcag
    22861 cgtcttccag cgcaggaacg gcatcaacaa aggccactga agcctcaaaa agtgctgccg
    22921 ctgcagagtc ctcaaaaagc gtggcagcta ccagtgccgg tgcggcgaaa acgtcagaaa
    22981 cgaatgccgc agcgtcagaa caatcagccg ccacttctgc atccaccgcg accacgaaag
    23041 cgtcagaagc tgccacctca gcccgggatg cggcggcctc aaaagaggca gcgaaatcat
    23101 cagaaacgaa cgcatccttg agcgccagta gcgcagcttc ctcggcaacg gcggcaggaa
    23161 attccgcgaa ggcggcaaaa acgtccgaga cgaacgccag gtcttctgag acggcagcgg
    23221 gacagagcgc ctcagctgcg gcaggctcaa aaacagcggc tgcgtcgtct gccagtgccg
    23281 cgtcaacaag tgccgggcag gcctcagcca gtgccaccgc cgccggaaaa tcggcagaaa
    23341 gcgccgcatc atccgcttca acagccacaa cgaaggctgg cgaagccact gagcaggcca
    23401 cggcagcagc gaggtcagct tccgcagcga agacatccga aacgaacgcg aaagcgtcgg
    23461 aaacccgtgc agaatcctca aaaacggcag cggcatcgtc cgccagttcg gcggcgtcat
    23521 cggcatcatc ggcgtctgct tcaaaagatg aggcgaccag acaggcgtca gcagcgaagg
    23581 gcagcgccac gacggcatcc acgaaggcga cagaggctgc tggtagtgcg aaggcagcag
    23641 ctcagagcaa aagtacggcg gaatctgcag caacgcgcgc tgagacagcg gcaaaacggg
    23701 cagaggatat tgcatccgcc gtggcgcttg aggatgcgag cacgacgaaa aaggggatag
    23761 tacagctcag tagtgcgact aacagcactt ccgagtcact ggcggcaaca ccaaaagccg
    23821 ttaaggccgc gtatgacctg gctagcggga aatacaccgc acaggatgca acgacagcac
    23881 agaaagggat aatccagcta agcagcgcga ccaacagcac gtctgaaacg ctggcggcaa
    23941 cgccaaaggc agtaaaagca gccaatgaca atgctgagaa acgtctgcag aaagatcaga
    24001 acggtgcgga tatccctggc aaagacacct ttacgaaaaa tattggtgcc tgccgtgcct
    24061 tcggtgggtc agtaagcaca acaacaggaa actggacgac tgcacagttt atcgagtggc
    24121 tggattctca gggagcattt aaccatccat actggatgtg caagggttcc tggtcttatg
    24181 gcaataataa aatcattact gatactggct gcggtaatat tcatctcgcc ggagctgtca
    24241 ttgaagtaat ggggataaag tcagcgatga cgatccgcat taccactccg accacatcca
    24301 gcggtggcgg aacaaccaac gcccagttta cctatattaa tcacggaaca gattattcac
    24361 ctggctggcg aagggactat aactccagaa ataagccaac ggcatcagag atcggggcgc
    24421 taccgtcagg tggaacagca gtatcatcag ttaatctggc ttcaaaaggt cgggtagccg
    24481 cgctgacaga caatacgcag ggggcagcag gtcttgagtt atacgaggtg tataacaacg
    24541 gatatccaac aacgtatgga aatatcattc acctgaaagg gatgacagcc gttggcgaag
    24601 gtgagttact catcggctgg agtggtacaa gcggtgctca tgctccggca tttattcgtt
    24661 cacgacggga tacgaccgac gcaaactggt cgccgtgggc gcagctttac acctcggctc
    24721 atcctcctgc agagttttat ccagtcggtg caccaatccc gtggccatca gataccgttc
    24781 cgtctggtta tgtcctgatg caggggcaga cttttgacaa atctgcatac ccgaaacttg
    24841 cagccgctta tccgtcaggc gtgatccctg atatgcgtgg ctggacgatt aagggcaaac
    24901 ctgccagtgg tcgggccgta ttgtctcagg aacaggacgg cattaaatcg cacacccaca
    24961 gcgccagcgc atccagtacg gatttgggga cgaaaaccac atcgtcgttt gattacggca
    25021 ctaaatccac gaataacatc ggggcgcata cgcacagtgt gagcggtaca gccgcaagtg
    25081 ccggaaacca tactcatagt gtcacaggcg catcagcagt cagccagtgg tcacaaaatg
    25141 ggtcagtaca taaggtagtg tctgcggcca gtgtgaatac aagtgctgca ggagcgcaca
    25201 ctcatagtgt cagcggcaca gctgcatctg caggtgctca cgcacatact gtcggtattg
    25261 gtgctcatac gcactcggtt gcgattggct cacatggaca caccatcacc gttaacgctg
    25321 ctggtaacgc ggaaaacacc gtcaaaaaca tcgcatttaa ctatattgtg aggcttgcat
    25381 gattacgctc attctttctg caccagtaac cgaaatggct gaagcattta agcgggtatt
    25441 tgcaaatgca gataatgtga atattgtcgg aaagccattt gaaacaatca gagaatttga
    25501 ctgcatggta agtgcggcaa atagtttcgg cctgatggat ggcggtgttg atgccgccat
    25561 taccacattc ttcggttctc agctacaggc ccgcgttcag aatcatattc ttcgtgaata
    25621 tctcggcgag cagcctgtag gttctgcatt tgttattgaa acggggcata attatcaccc
    25681 ctggctggta catgcgccaa caatgcgtgt tccgttgaca attgacggaa cagacgctgt
    25741 atataacgca acttgggcag ctctacttgc tatctttcag cacaataaaa acgcaacgac
    25801 agacaggaaa ataaagacgg tggtattccc tgcaatggga gccggatgtg gtcaggtgcc
    25861 gtttgaaagc gttgcccggc agatgaagca ggcatgggat aactttaata aaaaaacaga
    25921 atcaattaac tgggaatacg cacaatcccg ccagtcggca gtatttggca catatgcata
    25981 ctgtccgggt aattctgttt gccgttatgc ggatactaaa tatattggat gcggcgatta
    26041 tcggacgtat tgctcacgtt ccgggcaggt ctgtattaac cctgaacatc aggctgatga
    26101 tgtactgata caacatcagg ctaataaccg gtttcgccct ggttcgcata tacaccggat
    26161 taatccagaa aatcctgtag gtaatgtcat ctctggcgca catagccacg gaagtagcat
    26221 cgttattggt gctcacaccc atacgctcaa taaacaatat tctgtctctg atattaagta
    26281 gaggtgaaca tggatttcag aatgagtgaa caaccacgga ccataacaat ttataatctg
    26341 ctggccggaa ctaatgaatt tattggtgaa ggtgacgcat acattccgcc tcatacaggt
    26401 ctgccagcaa acagtaccga tattgcaccg ccagatattc cggctggttt cgtggctgtt
    26461 ttcaacagtg atgagtcatc gtggcatctc gttgaagatc atcggggtaa aacggtttat
    26521 gacgtggctt ccggcgacgc gttatttatt tctgaactcg gctcattacc ggaaaatgtc
    26581 acctggttat ccccggaagg ggagtttcag aagtggaacg gcacagcctg ggtgaaggat
    26641 acggaagcag aaaaactgtt ccggatccgg gaggcggaag aaacaaaaaa cagcctgatg
    26701 caggtagcca gtgagcatat agcgccgctt caggatgctg tggatctgga gatcgcaacg
    26761 gagaaagaaa ccttgttgct ggaagcctgg aaaaagtatc gggtgttgct gaaccgtgtt
    26821 gatacatcaa ctgcaccgga ta
//
LOCUS       DAZFLD010000050        26481 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.050, whole genome shotgun sequence.
ACCESSION   DAZFLD010000050 DAZFLD010000000
VERSION     DAZFLD010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26481
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.050"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1463
                     /gene="gspA"
                     /locus_tag="ACHKBL_001734"
     CDS             <1..1463
                     /gene="gspA"
                     /locus_tag="ACHKBL_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type II secretion system protein GspA"
                     /protein_id="HFO5818614.1"
                     /translation="RREVILSWLREKRQTWRLCYLLGEAGSGKTWLAQQLQKDKHRRV
                     ITLSLVVSSQGKAAWIVTDDNAAEQGCRDSAWTRDEMAGQLLHALHRTDSRCPLIIIE
                     NAHLNHRRILDDLQRAISLIPDGQFLLIGRPDRKVERDFKKQGIELVSTGRLMEHELK
                     ASILEGQNIDQPDLLLTARVLKRIALLCRGDRRKLALAGETISLLQQAEQTSVFTAKH
                     WRMIYRVLGDNRPRKMQLAVVMSGTIIALTCGWLLLSSFTDPFPVPAWLVPVTPVVKQ
                     DMTKDIAHVVMRDSEALSVLYGVWGYEVPADSAWCDQAVRAGLACKSGNASLQTLVDQ
                     NLPWIASLKVGDKKLPVVVVRVGDATVDVLVGQQTWTLTHQWFESVWTGDYLLLWKMS
                     PEGESTITRDSSEEEILWLETMLNRALHISTEPSAEWRPLLVEKIKQFQKSHHLKTDG
                     VVGFSTLVHLWQVAGESAYLYRDEANISPETTVKGK"
     gene            1465..1884
                     /gene="gspB"
                     /locus_tag="ACHKBL_001735"
     CDS             1465..1884
                     /gene="gspB"
                     /locus_tag="ACHKBL_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative general secretion pathway protein GspB"
                     /protein_id="HFO5818615.1"
                     /translation="MFEFYIAAREQKETGHPGIFSRQKHSTIIYVICLLLICLWFAGM
                     VLVGGYARQLWALWIVKAEVTVEAETPAFKQSTQHYFFKKQPLPVVESVEEEDDPDVA
                     VENAPSSSEDEENTVEESDEKAGLRERVKNALNELER"
     gene            2122..2433
                     /gene="rpsJ"
                     /locus_tag="ACHKBL_001736"
     CDS             2122..2433
                     /gene="rpsJ"
                     /locus_tag="ACHKBL_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HFO5818616.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
     gene            2466..3095
                     /gene="rplC"
                     /locus_tag="ACHKBL_001737"
     CDS             2466..3095
                     /gene="rplC"
                     /locus_tag="ACHKBL_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HFO5818617.1"
                     /translation="MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDG
                     YRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFA
                     DVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKK
                     MAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA"
     gene            3106..3711
                     /gene="rplD"
                     /locus_tag="ACHKBL_001738"
     CDS             3106..3711
                     /gene="rplD"
                     /locus_tag="ACHKBL_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HFO5818618.1"
                     /translation="MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTR
                     AQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYR
                     GALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
                     ARNLHKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA"
     gene            3708..4010
                     /gene="rplW"
                     /locus_tag="ACHKBL_001739"
     CDS             3708..4010
                     /gene="rplW"
                     /locus_tag="ACHKBL_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HFO5818619.1"
                     /translation="MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIK
                     AAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDFVGGAE"
     gene            4028..4849
                     /gene="rplB"
                     /locus_tag="ACHKBL_001740"
     CDS             4028..4849
                     /gene="rplB"
                     /locus_tag="ACHKBL_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HFO5818620.1"
                     /translation="MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRN
                     NNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGT
                     YVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK"
     gene            4866..5144
                     /gene="rpsS"
                     /locus_tag="ACHKBL_001741"
     CDS             4866..5144
                     /gene="rpsS"
                     /locus_tag="ACHKBL_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HFO5818621.1"
                     /translation="MPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNM
                     IGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTYRGHAADKKAKKK"
     gene            5159..5491
                     /gene="rplV"
                     /locus_tag="ACHKBL_001742"
     CDS             5159..5491
                     /gene="rplV"
                     /locus_tag="ACHKBL_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HFO5818622.1"
                     /translation="METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAA
                     VLVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            5509..6210
                     /gene="rpsC"
                     /locus_tag="ACHKBL_001743"
     CDS             5509..6210
                     /gene="rpsC"
                     /locus_tag="ACHKBL_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HFO5818623.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLT
                     KELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQIN
                     IAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGA
                     EIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPE
                     KPAAQPKKQQRKGRK"
     gene            6223..6633
                     /gene="rplP"
                     /locus_tag="ACHKBL_001744"
     CDS             6223..6633
                     /gene="rplP"
                     /locus_tag="ACHKBL_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HFO5818624.1"
                     /translation="MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTAR
                     QIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM"
     gene            6633..6824
                     /gene="rpmC"
                     /locus_tag="ACHKBL_001745"
     CDS             6633..6824
                     /gene="rpmC"
                     /locus_tag="ACHKBL_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HFO5818625.1"
                     /translation="MKAKELREKSVEELNTELLNLLREQFNLRMQAASGQLQQSHLLK
                     QVRRDVARVKTLLNEKAGA"
     gene            6824..7078
                     /gene="rpsQ"
                     /locus_tag="ACHKBL_001746"
     CDS             6824..7078
                     /gene="rpsQ"
                     /locus_tag="ACHKBL_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HFO5818626.1"
                     /translation="MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKL
                     HVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAVL"
     gene            7243..7614
                     /gene="rplN"
                     /locus_tag="ACHKBL_001747"
     CDS             7243..7614
                     /gene="rplN"
                     /locus_tag="ACHKBL_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HFO5818627.1"
                     /translation="MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            7625..7939
                     /gene="rplX"
                     /locus_tag="ACHKBL_001748"
     CDS             7625..7939
                     /gene="rplX"
                     /locus_tag="ACHKBL_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HFO5818628.1"
                     /translation="MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIVEGINLVKK
                     HQKPVPALNQPGGIVEKEAAIQVSNVAIFNAATGKADRVGFRFEDGKKVRFFKSNSET
                     IK"
     gene            7954..8493
                     /gene="rplE"
                     /locus_tag="ACHKBL_001749"
     CDS             7954..8493
                     /gene="rplE"
                     /locus_tag="ACHKBL_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HFO5818629.1"
                     /translation="MAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAI
                     ADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTA
                     KSDEEGRALLAAFDFPFRK"
     gene            8508..8813
                     /gene="rpsN"
                     /locus_tag="ACHKBL_001750"
     CDS             8508..8813
                     /gene="rpsN"
                     /locus_tag="ACHKBL_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFO5818630.1"
                     /translation="MAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNA
                     VLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            8847..9239
                     /gene="rpsH"
                     /locus_tag="ACHKBL_001751"
     CDS             8847..9239
                     /gene="rpsH"
                     /locus_tag="ACHKBL_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HFO5818631.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEG
                     FIEDFKVEGDTKPELELTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            9252..9785
                     /gene="rplF"
                     /locus_tag="ACHKBL_001752"
     CDS             9252..9785
                     /gene="rplF"
                     /locus_tag="ACHKBL_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HFO5818632.1"
                     /translation="MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVK
                     HADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNV
                     INLSLGFSHPVDHQLPAGITAECPTQTEIVLKGADKQVIGQVAADLRAYRRPEPYKGK
                     GVRYADEVVRTKEAKKK"
     gene            9795..10148
                     /gene="rplR"
                     /locus_tag="ACHKBL_001753"
     CDS             9795..10148
                     /gene="rplR"
                     /locus_tag="ACHKBL_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HFO5818633.1"
                     /translation="MDKKSARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNG
                     SEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGR
                     VQALADAAREAGLQF"
     gene            10163..10666
                     /gene="rpsE"
                     /locus_tag="ACHKBL_001754"
     CDS             10163..10666
                     /gene="rpsE"
                     /locus_tag="ACHKBL_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HFO5818634.1"
                     /translation="MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNG
                     RVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKS
                     VEEILGK"
     gene            10670..10849
                     /gene="rpmD"
                     /locus_tag="ACHKBL_001755"
     CDS             10670..10849
                     /gene="rpmD"
                     /locus_tag="ACHKBL_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HFO5818635.1"
                     /translation="MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAI
                     RGMINAVSFMVKVEE"
     gene            10853..11287
                     /gene="rplO"
                     /locus_tag="ACHKBL_001756"
     CDS             10853..11287
                     /gene="rplO"
                     /locus_tag="ACHKBL_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HFO5818636.1"
                     /translation="MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGG
                     GVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEVRLSDLAKVEGGVVDLNTLKAANIIG
                     IQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE"
     gene            11295..12626
                     /gene="secY"
                     /locus_tag="ACHKBL_001757"
     CDS             11295..12626
                     /gene="secY"
                     /locus_tag="ACHKBL_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HFO5818637.1"
                     /translation="MAKQPGLDFQSAKGGLGELKRRLLFVIGALIVFRIGSFIPIPGI
                     DAAVLAKLLEQQRGTIIEMFNMFSGGALSRASIFALGIMPYISASIIIQLLTVVHPTL
                     AEIKKEGESGRRKISQYTRYGTLVLAIFQSIGIATGLPNMPGMQGLVINPGFAFYFTA
                     VVSLVTGTMFLMWLGEQITERGIGNGISIIIFAGIVAGLPPAIAHTIEQARQGDLHFL
                     VLLLVAVLVFAVTFFVVFVERGQRRIVVNYAKRQQGRRVYAAQSTHLPLKVNMAGVIP
                     AIFASSIILFPATIASWFGGGTGWNWLTTISLYLQPGQPLYVLLYASAIIFFCFFYTA
                     LVFNPRETADNLKKSGAFVPGIRPGEQTAKYIDKVMTRLTLVGALYITFICLIPEFMR
                     DAMKVPFYFGGTSLLIVVVVIMDFMAQVQTLMMSSQYESALKKANLKGYGR"
     gene            12658..12774
                     /gene="rpmJ"
                     /locus_tag="ACHKBL_001758"
     CDS             12658..12774
                     /gene="rpmJ"
                     /locus_tag="ACHKBL_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HFO5818638.1"
                     /translation="MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG"
     gene            12921..13277
                     /gene="rpsM"
                     /locus_tag="ACHKBL_001759"
     CDS             12921..13277
                     /gene="rpsM"
                     /locus_tag="ACHKBL_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HFO5818639.1"
                     /translation="MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVK
                     ISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            13294..13683
                     /gene="rpsK"
                     /locus_tag="ACHKBL_001760"
     CDS             13294..13683
                     /gene="rpsK"
                     /locus_tag="ACHKBL_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HFO5818640.1"
                     /translation="MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGW
                     ATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            13717..14337
                     /gene="rpsD"
                     /locus_tag="ACHKBL_001761"
     CDS             13717..14337
                     /gene="rpsD"
                     /locus_tag="ACHKBL_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HFO5818641.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGAR
                     KPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVV
                     YRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALE
                     LAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK"
     gene            14363..15352
                     /gene="rpoA"
                     /locus_tag="ACHKBL_001762"
     CDS             14363..15352
                     /gene="rpoA"
                     /locus_tag="ACHKBL_001762"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HFO5818642.1"
                     /translation="MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTL
                     NKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIH
                     SEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIR
                     RAATILAEQLEAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIADE"
     gene            15393..15776
                     /gene="rplQ"
                     /locus_tag="ACHKBL_001763"
     CDS             15393..15776
                     /gene="rplQ"
                     /locus_tag="ACHKBL_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HFO5818643.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKEL
                     RRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFASRAGGYTRILKCGF
                     RAGDNAPMAYIELVDRSEKAEAAAE"
     gene            15883..16251
                     /locus_tag="ACHKBL_001764"
     CDS             15883..16251
                     /locus_tag="ACHKBL_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1992 domain-containing protein"
                     /protein_id="HFO5818644.1"
                     /translation="MWLLDQWAERHIAEAQAKGEFDNLAGSGEPLILADDSHVPPELR
                     AGYRLLKNAGCLPPELEQRREAIQLLDILRGIRHDDPQYQEVSRRLSLLELKLRQAGL
                     STDFLRGDYADKLLNKINDN"
     gene            16262..16687
                     /gene="zntR"
                     /locus_tag="ACHKBL_001765"
     CDS             16262..16687
                     /gene="zntR"
                     /locus_tag="ACHKBL_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(2+)-responsive transcriptional regulator"
                     /protein_id="HFO5818645.1"
                     /translation="MYRIGELAKMAEVTPDTIRYYEKQQMMEHEVRTEGGFRLYTESD
                     LQRLKFIRHARQLGFSLESIRELLSIRIDPEHHTCQESKGIVQERLQEVEARIAELQS
                     MQRSLQRLNDACCGTAHSSVYCSILEALEQGASGVKSGC"
     gene            16743..16961
                     /gene="arfA"
                     /locus_tag="ACHKBL_001766"
     CDS             16743..16961
                     /gene="arfA"
                     /locus_tag="ACHKBL_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome-rescue factor ArfA"
                     /protein_id="HFO5818646.1"
                     /translation="MSRYQHTKGQIKDNAIEALLHDPLFRQRVERNKKGKGSYMRKGK
                     HGNRGNWEASGKKVNHFFTTGLLLSGAC"
     gene            complement(16958..17368)
                     /gene="mscL"
                     /locus_tag="ACHKBL_001767"
     CDS             complement(16958..17368)
                     /gene="mscL"
                     /locus_tag="ACHKBL_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HFO5818647.1"
                     /translation="MSIIKEFREFAMRGNVVDLAVGVIIGAAFGKIVSSLVADIIMPP
                     LGLLIGGIDFKQFAVTLRDAQGDIPAVVMHYGVFIQNVFDFLIVAFAIFMAIKLINKL
                     NRKKEEPAAAPTPTKEEVLLTEIRDLLKEQNNRS"
     gene            complement(17498..18874)
                     /gene="trkA"
                     /locus_tag="ACHKBL_001768"
     CDS             complement(17498..18874)
                     /gene="trkA"
                     /locus_tag="ACHKBL_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HFO5818648.1"
                     /translation="MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFD
                     LRVVQGHGSHPRVLREAGADDADMLVAVTSSDETNMVACQVAYSLFNTPNRIARIRSP
                     DYVRDADKLFHSDAVPIDHLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKA
                     YYGGPLIGNALSTMREHMPHIDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHI
                     RAVMSELQRLEKPYKRIMLVGGGNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQN
                     TIVFFGDASDQELLAEEHIDQVDLFIAVTNDDEANIMSAMLAKRMGAKKVMVLIQRRA
                     YVDLVQGSVIDIAISPQQATISALLSHVRKADIVGVSSLRRGVAEAIEAVAHGDESTS
                     RVVGRVIDEIKLPPGTIIGAVVRGNDVMIANDNLRIEQGDHVIMFLTDKKFITDVERL
                     FQPSPFFL"
     gene            complement(18896..20185)
                     /gene="rsmB"
                     /locus_tag="ACHKBL_001769"
     CDS             complement(18896..20185)
                     /gene="rsmB"
                     /locus_tag="ACHKBL_001769"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HFO5818649.1"
                     /translation="MKKQRNLRSMAAQAVEQVVEQGQSLSNILPPLQQKVSDKDKALL
                     QELCFGVLRTLSQLNWLINKLMARPMTGKQRTVHYLIMVGLYQLLYTRIPPHAALAET
                     VEGAIAIKRPQLKGLINGVLRQFQRQQEELLAEFNASDARYLHPSWLLKRLQKAYPEQ
                     WQSIVEANNQRPPMWLRVNRTHHSRDSWLALLDEAGMKGFPPADYPDAVRLETPAPVH
                     ALPGFEDGWVTVQDASAQGCMTWLAPQNGEHILDLCAAPGGKTTHILEVAPEAQVVAV
                     DIDEQRLSRVYDNLKRLGMKATVKQGDGRYPSQWCGEQQFDRILLDAPCSATGVIRRH
                     PDIKWLRRDRDIPELAQLQSEILDAIWPHLKSGGTLVYATCSVLPEENSLQIKAFLQR
                     TADAELCETGTPEQPGKQNLPGAEEGDGFFYAKLIKK"
     gene            complement(20231..21178)
                     /gene="fmt"
                     /locus_tag="ACHKBL_001770"
     CDS             complement(20231..21178)
                     /gene="fmt"
                     /locus_tag="ACHKBL_001770"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HFO5818650.1"
                     /translation="MSESLRIIFAGTPDFAARHLDALLSSGHNVVGVFTQPDRPAGRG
                     KKLMPSPVKVLAEEKGLPVFQPVSLRPQENQQLVADLQADVMVVVAYGLILPKAVLEM
                     PRLGCINVHGSLLPRWRGAAPIQRSLWAGDAETGVTIMQMDVGLDTGDMLYKLSCPIT
                     AEDTSGTLYDKLAELGPQGLITTLKQLADGTAKPEVQDETLVTYAEKLSKEEARIDWS
                     LSAAQLERCIRAFNPWPMSWLEIEGQPVKVWKASVIDTATNAAPGTILEANKQGIQVA
                     TGDGILNLLSLQPAGKKAMSAQDLLNSRREWFVPGNRLV"
     gene            complement(21193..21702)
                     /gene="def"
                     /locus_tag="ACHKBL_001771"
     CDS             complement(21193..21702)
                     /gene="def"
                     /locus_tag="ACHKBL_001771"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFO5818651.1"
                     /translation="MSVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEG
                     IGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVP
                     RAEKVKIRALDRDGKPFELEAEGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVE
                     KLDRLKARA"
     gene            21832..22956
                     /gene="dprA"
                     /locus_tag="ACHKBL_001772"
     CDS             21832..22956
                     /gene="dprA"
                     /locus_tag="ACHKBL_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HFO5818652.1"
                     /translation="MVDTEIWLRLMSISSLYGDDMVRIAHWLAKQSHIDAVVLQQTGL
                     TLRQAQRFLSFPRKSIESSLSWLEQPNHHLIPADSEFYPPQLLATTDYPGALFVEGEL
                     HALHSFQLAVVGSRAHSWYGERWGRLFCETLATRGVTITSGLARGIDGVAHKAALQVN
                     GVSIAVLGNGLNTIHPRRHARLATSLLEHGGALVSEFPLDVPPLAYNFPRRNRIISGL
                     SKGVLVVEAALRSGSLVTARCALEQGREVFALPGPLGNPGSEGPHWLIKQGAILVTEP
                     EEILENLQFGLHWLPDAPENSFYSPDQEDVALPFPELLANVGDEVTPVDVVAERAGQP
                     VPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV"
     gene            22928..23401
                     /gene="smg"
                     /locus_tag="ACHKBL_001773"
     CDS             22928..23401
                     /gene="smg"
                     /locus_tag="ACHKBL_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 family protein Smg"
                     /protein_id="HFO5818653.1"
                     /translation="MFDVLMYLFETYIHTEAELRVDQDKLEQDLTDAGFDREDIYNAL
                     LWLEKLADYQEGLAEPMQLASDPLSMRIYTPEECERLDASCRGFLLFLEQIQVLNLET
                     REMVIERVLALDTAEFDLEDLKWVILMVLFNIPGCENAYQQMEELLFEVNEGMLH"
     gene            23430..23972
                     /locus_tag="ACHKBL_001774"
     CDS             23430..23972
                     /locus_tag="ACHKBL_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
                     /protein_id="HFO5818654.1"
                     /translation="MAKSALFTVRNNESCPKCGAELVIRSGKHGPFLGCSQYPACDYV
                     RPLKSSADGHIVKVLEGQVCPACGANLVLRQGRFGMFIGCSNYPECEHTELIDKPDET
                     AITCPQCRTGHLVQRRSRYGKTFHSCDRYPECQFAINFKPIAGECPECHYPLLIEKKT
                     AQGVKHFCASKQCGKPVSAE"
     gene            23977..24549
                     /gene="tsaC"
                     /locus_tag="ACHKBL_001775"
     CDS             23977..24549
                     /gene="tsaC"
                     /locus_tag="ACHKBL_001775"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase type 1
                     TsaC"
                     /protein_id="HFO5818655.1"
                     /translation="MNNNLQGDAIAAAIDVLNEKRVIAYPTEAVFGVGCDPDSETAVM
                     RLLELKQRPVDKGLILIAANYEQLKPYIDDTMLTDAQRETIFSRWPGPVTFVFPAPAT
                     TPRWLTGRFDSLAVRVTDHPLVVALCQAYGKPLVSTSANLSGLPPCRTVDEVRAQFGA
                     AFPVVPGETGGRLNPSEIRDALTGELFRQG"
     gene            24554..25372
                     /gene="aroE"
                     /locus_tag="ACHKBL_001776"
     CDS             24554..25372
                     /gene="aroE"
                     /locus_tag="ACHKBL_001776"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HFO5818656.1"
                     /translation="METYAVFGNPIAHSKSPFIHQQFAQQLNIEHPYGRVLAPINDFI
                     NTLNAFFSAGGKGANVTVPFKEEAFARADELTERAALAGAVNTLKRLEDGRLLGDNTD
                     GIGLLSDLERLSFIRPGLRILLIGAGGASRGVLLPLLSLDCAVTITNRTVSRAEELAK
                     LFAHTGSIQALGMDELEGHEFDLIINATSSGISGDIPAIPSSLIHPGIYCYDMFYQKG
                     KTPFLAWCEQRGSKRNADGLGMLVAQAAHAFLLWHGVLPDVEPVIKLLQQELSA"
     gene            25369..25626
                     /locus_tag="ACHKBL_001777"
     CDS             25369..25626
                     /locus_tag="ACHKBL_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /protein_id="HFO5818657.1"
                     /translation="MNQAIQFPDREEWDENKRCVCFPALVNGMQLTCAISGESLAYRF
                     TGDTPEQWLASFRQHRWDLEEEAENLIQEQSEDDQGWVWLP"
     gene            complement(25602..26156)
                     /locus_tag="ACHKBL_001778"
     CDS             complement(25602..26156)
                     /locus_tag="ACHKBL_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HFO5818658.1"
                     /translation="MSDVLRPYRDLFPQIGQRVMIDDSSVVIGDVRLADDVGIWPLVV
                     IRGDVHYVQIGARTNIQDGSMLHVTHKSSYNPDGNPLTIGEDVTVGHKVMLHGCTIGN
                     RVLVGMGSILLDGAIVEDDVMIGAGSLVPQNKRLESGYLYLGSPVKQIRPLSDEEKAG
                     LRYSAKNYVKWKDEYLDQGNQTQP"
     gene            complement(26200..>26481)
                     /locus_tag="ACHKBL_001779"
     CDS             complement(26200..>26481)
                     /locus_tag="ACHKBL_001779"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5818659.1"
                     /translation="ATRLSAHCREVAYITLSSFRVNPEFQDFFSLLLRPSCEVFHVVV
                     STEAHYRDPNFLHKYFFDLFFCLLLFHPFCCIRTQNGAFLHAKRLAQGQ"
BASE COUNT         6736 a         6268 c         6929 g         6548 t
ORIGIN      
        1 cgagaagaga agttattctt tcctggttgc gtgagaaacg tcagacctgg cgtctgtgct
       61 atttgttggg tgaggctgga agtggaaaaa cctggctggc gcagcaacta caaaaagata
      121 aacatcgccg tgtgattact ttaagcctcg ttgtttcctc gcaaggtaag gccgcatgga
      181 tcgttaccga cgataatgcg gctgaacagg gctgccgtga cagcgcctgg acgcgagatg
      241 agatggcggg tcaattactg catgcgcttc atcgaactga tagtcgctgc cctcttatta
      301 ttattgaaaa tgctcacctg aatcaccgcc gcatacttga tgatctccaa cgcgcgatat
      361 ctcttattcc tgacggtcaa tttttgctga tcggcagacc cgatcgaaaa gtcgaacgtg
      421 attttaaaaa acagggcatt gaacttgtct caacaggacg tctgatggag cacgagctta
      481 aggcaagcat ccttgaaggt cagaatattg accagcccga tctcctttta actgccagag
      541 ttctgaaacg gatagcttta ttatgtcggg gagatcgcag aaagctggcg cttgccggcg
      601 aaacaattag cttattacag caagccgagc agaccagcgt tttcactgca aaacattggc
      661 gaatgattta ccgcgtatta ggtgataacc gcccccggaa aatgcagctt gccgtggtga
      721 tgagtggaac cattatcgca cttacctgtg gttggctact gctgtcctct ttcacagatc
      781 cgttccccgt tcctgcgtgg ctggtacccg taacgccagt agttaaacaa gatatgacta
      841 aggatattgc tcatgtagtg atgcgcgata gtgaggcgct aagcgtgtta tacggcgtat
      901 gggggtacga ggtcccagct gattcggcct ggtgcgatca ggcagtaaga gcggggctgg
      961 catgtaaaag tgggaatgct tctttacaaa cattggtaga tcagaatttg ccctggatcg
     1021 catcgcttaa agtcggggac aaaaaattgc ctgttgtggt cgttcgcgtc ggtgatgcta
     1081 ccgttgatgt cctcgtaggg cagcaaacct ggacgctgac acatcaatgg tttgagtcgg
     1141 tatggacggg tgattatctt ctgttatgga aaatgtcgcc ggaaggggag agtaccatta
     1201 cgcgtgatag cagcgaggaa gagattctct ggctggaaac gatgttaaac cgcgcattgc
     1261 atatttcgac agaaccttcg gcagagtggc gtcccttgtt ggtagaaaaa ataaagcaat
     1321 ttcaaaaaag ccaccacttg aaaactgacg gtgttgttgg cttttcaacg ttagtacatc
     1381 tctggcaggt ggcaggggag agtgcctact tatatcggga tgaagcaaat atttccccgg
     1441 aaacaacagt gaaggggaaa tgacatgttt gaattctata tcgccgcgcg tgagcagaaa
     1501 gaaacaggtc accctggtat cttctcccga caaaaacaca gcaccataat atatgtcatt
     1561 tgtttattgc tgatttgcct gtggtttgca ggaatggtgt tggttggtgg gtatgccagg
     1621 cagctatggg cgctttggat agtaaaagca gaagtcactg tagaggctga aacgcccgct
     1681 tttaaacaat caacacaaca ctatttcttc aaaaaacagc cactccccgt cgtcgaatcc
     1741 gtcgaggagg aagatgaccc agacgtcgcg gttgagaatg ctccctcttc ttctgaagac
     1801 gaagaaaata ctgtagaaga aagcgacgag aaagccggtt taagagagcg tgtcaaaaat
     1861 gcgctgaacg aacttgagag ataacccgaa ggctgtttac tttactagca atacgcttgc
     1921 gttcggtggt taagtatgta taatgcgcgg gcttgtcgta gttgacagca ggttcaatct
     1981 gaacctcagt tagccgaatt tggctaccta acaatgctcc caatcgggga gctacgtaag
     2041 aacggttaca ctctcccatc aatcgtaatg ggtctgagga gtaatcattt tcgtttataa
     2101 aataattgga gctctggtct catgcagaac caaagaatcc gtatccgcct gaaagcgttt
     2161 gatcatcgtc tgatcgatca agcaaccgcg gaaatcgtcg agactgccaa gcgcactggt
     2221 gcgcaggtcc gtggtccgat cccgctgccg acacgcaaag agcgcttcac tgttctgatc
     2281 tccccgcacg tcaacaaaga cgcgcgcgat cagtacgaaa tccgtactca cttgcgtctg
     2341 gttgacatcg ttgagccaac cgagaaaacc gttgatgctc tgatgcgtct ggatctggct
     2401 gccggtgtag acgtgcagat cagcctgggt taatcaggtc attgagcgat tgagaggttg
     2461 aaacaatgat tggtttagtc ggtaaaaaag tgggtatgac ccgtatcttc acagaagacg
     2521 gcgtttctat cccagtaacc gtaatcgaag ttgaagcaaa ccgcgttact caggttaaag
     2581 acctggctaa cgatggctac cgtgctattc aggtaaccac cggtgctaaa aaagctaacc
     2641 gtgtgaccaa gcctgaagct ggccacttcg ctaaagctgg cgtagaagct ggccgtggtc
     2701 tgtgggaatt ccgcctggct gaaggcgaag agttcactgt aggtcagagc attagcgttg
     2761 aactgtttgc tgacgttaaa aaagttgacg taactggcac ctctaaaggt aaaggtttcg
     2821 caggtaccgt taagcgctgg aacttccgta cccaggacgc tactcacggt aactccttgt
     2881 ctcaccgcgt tccgggttct atcggtcaga accagactcc gggcaaagtg ttcaaaggca
     2941 agaaaatggc aggtcagatg ggtaacgaac gtgtaaccgt tcagagcctt gacgtagtac
     3001 gcgttgacgc tgagcgcaac ctgctgctgg ttaaaggtgc tgtcccgggt gcaaccggta
     3061 gcgacctgat cgttaaacca gctgtgaagg cgtaaggaga tagcaatgga attagtattg
     3121 aaagacgcgc agagcgcgct gactgtttcc gaaactacct tcggtcgtga tttcaacgaa
     3181 gcgctggttc accaggttgt tgttgcttat gcagctggtg ctcgtcaggg tactcgtgct
     3241 cagaagactc gtgctgaagt aactggttcc ggtaaaaaac cgtggcgcca gaaaggcacc
     3301 ggccgtgcgc gttctggttc tatcaagagc ccgatctggc gttctggtgg cgtgaccttt
     3361 gctgctcgtc cgcaggacca cagtcaaaaa gttaacaaga agatgtaccg cggcgcgctg
     3421 aaaagcatcc tgtccgaact ggtacgtcag gatcgtctga tcgttgtcga gaagttctct
     3481 gtagaagcgc cgaaaactaa gctgctggca cagaaactga aagacatggc tctggaagat
     3541 gtgctgatca tcaccggtga gctggacgaa aacctgttcc tggctgcgcg caacctgcac
     3601 aaggttgacg tacgcgatgc aactggtatc gacccggtta gcctgatcgc cttcgacaaa
     3661 gtcgtaatga ctgctgatgc tgttaagcaa gttgaggaga tgctggcatg attcgtgaag
     3721 aacgtctgct gaaggtgctg cgtgcaccgc acgtttctga aaaagcgtct actgcgatgg
     3781 aaaaatctaa caccatcgta ctcaaagttg ctaaagacgc gaccaaagca gaaatcaaag
     3841 ctgctgtgca gaaactgttt gaagtcgaag tcgaagtcgt taacaccctg gtagttaaag
     3901 ggaaagttaa acgtcacgga cagcgtatcg gtcgtcgtag cgactggaaa aaagcttacg
     3961 tcaccctgaa agaaggccag aatctggact tcgttggcgg cgctgagtaa gtcggaggag
     4021 taatacaatg gcagttgtta aatgtaaacc gacatctccg ggtcgtcgcc acgttgttaa
     4081 agtggttaac cctgagctgc acaagggcaa accttttgct ccgttgctgg aaaaaaacag
     4141 caaatccggt ggtcgtaaca acaatggccg tatcaccact cgtcatatcg gtggtggcca
     4201 caagcaggct taccgtattg ttgacttcaa acgcaacaaa gacggtatcc cggcagttgt
     4261 tgaacgtctt gagtacgatc cgaaccgttc cgcgaacatc gcgctggttt tgtacaaaga
     4321 cggtgaacgc cgttacatcc tggcccctaa aggcctgaaa gctggcgacc agattcagtc
     4381 tggcgttgat gctgcaatca aaccaggtaa caccctgccg atgcgcaaca tcccggttgg
     4441 ttctactgtt cataacgtag aaatgaaacc aggtaaaggc ggtcagctgg cacgttccgc
     4501 tggtacttac gttcagatcg ttgctcgtga tggtgcttat gtcaccctgc gtctgcgttc
     4561 tggtgaaatg cgtaaagtag aagcagactg ccgtgcaact ctgggcgaag ttggcaatgc
     4621 tgagcatatg ctgcgcgttc tgggtaaagc aggtgctgca cgctggcgtg gtgttcgtcc
     4681 gaccgttcgc ggtaccgcga tgaacccggt agaccaccca catggtggtg gtgaaggtcg
     4741 taactttggt aagcacccgg taactccgtg gggcgttcag accaaaggta agaagacccg
     4801 cagcaacaag cgtactgata aattcatcgt acgtcgccgt agcaaataat tttagaggat
     4861 aagccatgcc acgttctctc aagaaaggtc cttttattga cctgcacttg ctgaagaagg
     4921 tagagaaagc ggtggaaagc ggagacaaga agcccctgcg cacttggtcc cgtcgttcaa
     4981 cgatctttcc taacatgatc ggtttgacca tcgctgtcca taatggtcgt cagcacgttc
     5041 cggtatttgt aaccgacgaa atggtcggtc acaaactggg tgaattcgca ccgactcgta
     5101 cttatcgcgg ccacgctgct gataaaaaag cgaagaagaa ataaggtagg aggaagagat
     5161 ggaaactatc gctaaacatc gccatgctcg ttcttctgct cagaaggttc gccttgttgc
     5221 tgacctgatt cgcggtaaga aagtgtcgca ggctctggat attttgacct acaccaacaa
     5281 gaaagcggct gtactggtca agaaagttct ggaatctgcc attgctaacg ctgaacacaa
     5341 cgatggcgct gacattgacg atctgaaagt tacgaaaatt ttcgtagacg aaggcccgag
     5401 catgaagcgc attatgccgc gtgcaaaagg tcgtgcagat cgcatcctga agcgcaccag
     5461 ccacatcact gtggttgtgt ccgatcgctg agactctgga gactagcaat gggtcagaaa
     5521 gtacatccta atggtattcg cctgggtatt gtaaaaccat ggaactctac ttggtttgcg
     5581 aacaccaaag aattcgctga caacctggac agcgatttta aagtacgtca gtacctgact
     5641 aaggaactgg ctaaagcgtc cgtatctcgt atcgttatcg agcgtccggc taagagcatc
     5701 cgtgtaacca ttcacactgc tcgcccgggt atcgttatcg gtaaaaaagg tgaagacgta
     5761 gaaaaactgc gtaaggtcgt agcggacatc gctggcgttc ctgcacagat caacatcgcc
     5821 gaagttcgta agcctgaact ggacgcaaaa ctggttgctg acagcatcac ttctcagctg
     5881 gaacgtcgcg ttatgttccg tcgtgctatg aagcgtgctg tacagaacgc aatgcgtctg
     5941 ggcgctaaag gtattaaagt tgaagttagc ggccgtctgg gcggcgcaga aatcgcacgt
     6001 accgaatggt accgcgaagg tcgcgtaccg ctgcacactc tgcgtgctga catcgactac
     6061 aacacctctg aagcgcacac cacttacggt gtaatcggcg ttaaagtgtg gatcttcaaa
     6121 ggcgagatcc tgggtggtat ggctgctgtt gaacaaccgg aaaaaccggc tgctcagcct
     6181 aaaaagcagc agcgtaaagg ccgtaaataa ggagcgtcgc tgatgttaca accaaagcgt
     6241 acaaaattcc gtaaaatgca caaaggccgt aaccgcggtc tggcgcaggg tacggatgtt
     6301 agcttcggca gcttcggtct gaaagctgtt ggccgtggtc gtctgactgc ccgtcagatc
     6361 gaagcagcac gtcgtgctat gacccgtgca gttaagcgtc aaggtaagat ctggatccgt
     6421 gtgttcccgg acaaaccgat cactgaaaag ccgctggcag tgcgtatggg taaaggtaaa
     6481 ggtaacgtgg agtattgggt tgccttgatt cagccgggta aagtcctgta tgaaatggac
     6541 ggtgttccgg aagagctggc ccgtgaagca ttcaagctgg cagcagcgaa actgccgatt
     6601 aaaaccacct ttgtaactaa gacggtgatg taatgaaagc aaaagagctg cgtgagaaga
     6661 gcgttgaaga gctgaacacc gagctgctga acctgctgcg tgagcagttc aacctgcgta
     6721 tgcaggctgc aagtggccag ctgcaacagt ctcacctgtt gaagcaagtg cgtcgcgatg
     6781 tcgcacgcgt taagacttta ctgaacgaga aggcgggtgc gtaatgaccg ataaaatccg
     6841 tactctgcaa ggtcgcgttg ttagcgacaa aatggagaaa tccattgttg ttgctatcga
     6901 acgttttgtg aaacacccga tctacggtaa attcatcaag cgtacgacca aactgcacgt
     6961 acatgacgag aacaacgaat gcggtatcgg tgacgtggtt gaaatccgcg aatgccgtcc
     7021 gctgtccaag actaaatcct ggacgctggt tcgcgttgta gagaaagcgg ttctgtaata
     7081 cagtacactc tctcgatacg aataaacggc tcagaaatga gccgtttatt ttttctaccc
     7141 atatccttga agcggtgtta taataccgcg ccctcgatat ggggattttt aacgacctga
     7201 ttttcgggtc tcagtagtag ttgacattag cggagcacta aaatgatcca agaacagact
     7261 atgctgaacg tcgccgacaa ctccggtgca cgtcgcgtaa tgtgtatcaa ggttctgggt
     7321 ggctcgcacc gtcgctacgc aggcgtaggc gacatcatca agatcaccat caaagaagca
     7381 attccgcgtg gtaaggtcaa aaaaggtgat gtgctgaagg cggtagtggt gcgcaccaag
     7441 aagggtgttc gtcgcccgga cggttctgtc attcgcttcg atggtaatgc ttgtgttctt
     7501 ctgaacaaca acagcgagca gcctatcggt acgcgtattt ttgggccggt aactcgtgag
     7561 cttcgtagtg agaagttcat gaaaattatc tctctggcac cagaagtact ctaaggagcg
     7621 aatcatggca gcgaaaatcc gtcgtgatga cgaagttatc gtgttaaccg gtaaagataa
     7681 aggtaaacgc ggtaaagtta agaatgtcct gtcttccggc aaggtcattg ttgaaggtat
     7741 caacctggtt aagaaacatc agaagccggt tccggccctg aaccaaccgg gtggcatcgt
     7801 tgaaaaagaa gccgctattc aggtttccaa cgtagcaatc ttcaatgcgg caaccggcaa
     7861 ggctgaccgt gtaggcttta gattcgaaga cggtaaaaaa gtccgtttct tcaagtctaa
     7921 cagcgaaact atcaagtaat ttggagtagt acgatggcga aactgcatga ttactacaaa
     7981 gacgaagtag ttaaaaaact catgactgag tttaactaca attctgtcat gcaagtccct
     8041 cgggtcgaga agatcaccct gaacatgggt gttggtgaag cgatcgctga caaaaaactg
     8101 ctggataacg cagcagcaga cctggcagca atctccggtc aaaaaccgct gatcaccaaa
     8161 gcacgcaaat ctgttgcagg cttcaaaatc cgtcagggct atccgatcgg ctgtaaagta
     8221 actctgcgtg gcgaacgcat gtgggagttc tttgagcgcc tgatcactat tgctgtacct
     8281 cgtatccgtg acttccgtgg cctgtccgct aagtctttcg acggtcgtgg taactacagc
     8341 atgggtgtcc gtgagcagat catcttccca gaaatcgact acgataaagt cgaccgcgtt
     8401 cgtggtttgg atattaccat taccactact gcgaaatctg acgaagaagg ccgcgctctg
     8461 ctggctgcct ttgacttccc gttccgcaag taaggtaggg ttactaaatg gctaagcaat
     8521 caatgaaagc acgcgaagta aaacgcgtag ctttagctga taaatacttc gcgaaacgcg
     8581 ctgaactgaa agcgatcatc tctgatgtga acgcttccga cgaagatcgt tggaacgctg
     8641 ttctcaagct gcagactctg ccgcgtgatt ccagcccgtc tcgtcagcgt aaccgctgcc
     8701 gtcaaacagg tcgtccgcat ggtttcctgc ggaagttcgg gttgagccgt attaaggtcc
     8761 gtgaagccgc tatgcgcggt gaaatcccgg gtctgaaaaa ggctagctgg taattgtcac
     8821 caattgaatc acgggagtaa agacagatga gcatgcaaga tccgatcgcg gatatgctga
     8881 cccgtatccg taacggtcag gccgcgaaca aagctgcggt caccatgcct tcctccaagc
     8941 tgaaagtggc aatcgccaac gtgctgaagg aagaaggttt tattgaagat tttaaagttg
     9001 aaggcgacac caagcctgaa ctggaactta ctctgaagta tttccagggc aaagctgttg
     9061 tagaaagcat tcagcgtgtc agccgcccag gtctgcgcat ctataagcgt aaagatgagc
     9121 tgccgaaagt tatggcgggt ctgggtatcg cagttgtttc tacctctaaa ggtgttatga
     9181 ctgatcgtgc agcgcgccag gctggtcttg gtggcgaaat tatctgctac gtagcctaat
     9241 cggaggaaaa aatgtctcgt gttgctaaag caccggtcgt tgttcctgcc ggcgttgacg
     9301 taaaaatcaa cggtcaggtt attacgatca aaggtaaaaa cggcgagctg actcgtactc
     9361 tcaacgatgc tgttgaagtt aaacatgcag ataataccct gaccttcggt ccgcgtgatg
     9421 gttacgcaga cggttgggca caggctggta ccgcgcgtgc cctgctgaac tcaatggtta
     9481 tcggtgttac cgaaggcttc actaagaagc tgcagctggt tggtgtaggt taccgtgcag
     9541 cggttaaagg caatgtgatt aacctgtctc tgggtttctc tcatcctgtt gaccatcagc
     9601 tgcctgcggg tatcactgct gaatgtccga ctcagactga aatcgtgctg aaaggcgctg
     9661 ataagcaggt gatcggccag gttgcagcgg atctgcgcgc ctaccgtcgt cctgagcctt
     9721 acaaaggcaa gggtgttcgt tacgccgacg aagtcgtgcg taccaaagag gctaagaaga
     9781 agtaaggtaa cactatggat aagaaatctg ctcgtatccg tcgtgcgacc cgcgcacgcc
     9841 gcaagctcca ggagctgggc gcaactcgcc tggtggtaca tcgtaccccg cgtcacattt
     9901 acgcacaggt aattgcaccg aacggttctg aagttctggt agctgcttct actgtagaaa
     9961 aagctatcgc tgaacaactg aagtacaccg gtaacaaaga cgcggctgca gctgtgggta
    10021 aagctgtcgc tgaacgcgct ctggaaaaag gcatcaaaga tgtatccttt gaccgttccg
    10081 ggttccaata tcatggtcgt gtccaggcac tggcagatgc tgcccgtgaa gctggccttc
    10141 agttctaagg tagaggtgta agatggctca catcgaaaaa caagctggcg aactgcagga
    10201 aaagctgatc gcggtaaacc gcgtatctaa aaccgttaaa ggtggtcgta ttttctcctt
    10261 cacagctctg actgtagttg gcgatggtaa cggtcgcgtt ggttttggtt acggtaaagc
    10321 gcgtgaagtt ccagcagcga tccagaaagc gatggaaaaa gcccgtcgca atatgattaa
    10381 cgtcgcgctg aataacggta ctctgcaaca ccctgttaaa ggtgttcata cgggttctcg
    10441 cgtattcatg cagccggctt ccgaaggtac cggtatcatc gccggtggtg caatgcgcgc
    10501 cgttctggaa gtcgctgggg ttcataacgt tctggctaaa gcgtatggtt ccaccaaccc
    10561 gattaacgtg gttcgtgcaa ctatcgatgg cctggaaaat atgaattccc cagaaatggt
    10621 cgctgccaag cgtggtaaat ccgttgaaga aattctgggg aaataaacca tggcaaagac
    10681 tattaaaatt actcaaaccc gcagtgcaat cggtcgtctg ccgaaacaca aggcaacgct
    10741 gcttggcctg ggtctgcgtc gtattggtca caccgtagag cgcgaggata ctcctgctat
    10801 tcgcggtatg atcaacgcgg tttccttcat ggttaaagtt gaggagtaag agatgcgttt
    10861 aaatactctg tctccggccg aaggctccaa aaaggcgggt aaacgcctgg gtcgtggtat
    10921 cggttctggc ctcggtaaaa ccggtggtcg tggtcacaaa ggtcagaaat ctcgttctgg
    10981 cggtggcgta cgtcgcggtt tcgagggtgg tcagatgcct ctgtaccgtc gtctgccgaa
    11041 attcggcttc acttctcgta aagcagcgat tacagccgaa gttcgtctgt ctgacctggc
    11101 taaagtagaa ggcggtgttg tagacctgaa cacgctgaaa gcggctaaca ttatcggtat
    11161 ccagatcgag ttcgcgaaag tgatcctggc tggcgaagtc actactccgg taactgttcg
    11221 tggcctgcgt gttactaaag gcgctcgtgc tgctatcgaa gctgctggcg gtaaaatcga
    11281 ggaataagta gcagatggct aaacaaccgg gattagattt tcaaagtgcc aaaggtggct
    11341 taggcgagct gaaacgcaga ctgctgtttg ttatcggtgc gctgattgtg ttccgtattg
    11401 gctcttttat tccgatccct ggtattgatg ccgctgtact tgccaaactg cttgagcaac
    11461 agcgaggcac catcattgaa atgtttaaca tgttctctgg tggtgctctc agccgtgctt
    11521 ctatctttgc tctggggatc atgccatata tttcggcatc gatcattatc cagcttctga
    11581 cggtggttca cccaacgttg gcagaaatta agaaagaagg ggagtctggt cgtcgtaaga
    11641 tcagccagta cacccgctac ggtactctgg tgctggcaat attccagtcg atcggtattg
    11701 ctaccggtct gccgaatatg cctggtatgc aaggcctggt cattaacccg ggctttgcat
    11761 tctacttcac cgctgttgta agtctggtca caggaaccat gttcctgatg tggttgggcg
    11821 aacagattac tgaacgaggt atcggcaacg gtatttcaat cattatcttc gccggtattg
    11881 tcgcgggact cccgccagcc attgcccata ctatcgagca agcgcgtcaa ggcgacctgc
    11941 acttcctcgt gttgctgttg gttgcagtat tagtatttgc agtgacgttc tttgttgtat
    12001 ttgttgagcg tggtcaacgc cgcattgtgg taaactacgc gaaacgtcag caaggtcgtc
    12061 gtgtctatgc tgcacagagc acacatttac cgctgaaagt gaatatggcg ggggtaatcc
    12121 cggcaatctt cgcttccagt attattctgt tcccggcgac catcgcgtca tggttcgggg
    12181 gcggtactgg ttggaactgg ctgacaacaa tttcgctgta tttgcagcct gggcaaccgc
    12241 tttatgtgtt actctatgcg tctgcaatca tcttcttctg tttcttctac acggcgttgg
    12301 ttttcaaccc gcgtgaaaca gcagataacc tgaagaagtc cggtgcattt gtaccaggaa
    12361 ttcgtccggg agagcaaacg gcgaagtata tcgataaagt aatgacccgc ctgaccctgg
    12421 ttggtgcgct gtacattacc tttatctgcc tgatcccgga gttcatgcgt gatgcaatga
    12481 aagtaccgtt ctactttggt gggacctcac tgcttatcgt tgttgtcgtg attatggact
    12541 ttatggctca agtgcaaact ctgatgatgt ccagtcagta tgagtctgca ttgaagaagg
    12601 cgaacctgaa aggctacggc cgataattgg tcgcccgaga agttacggag agtaaaaatg
    12661 aaagttcgtg cttccgtcaa gaaattatgc cgtaactgca aaatcgttaa gcgtgatggt
    12721 gtcatccgtg tgatttgcag tgccgagccg aagcataaac agcgccaagg ctgatttttt
    12781 cgcatatttt tcttgcaaag ttgggttgag ctggctagat tagccagcca atcttttgta
    12841 tgtctgtgcg tttccatttg agtatcctga aaacgggctt ttcagcatgg aacgtacata
    12901 ttaaatagta ggagtgcata gtggcccgta tagcaggcat taacattcct gatcataagc
    12961 atgccgtaat cgcattaact tcgatttatg gcgtcggcaa gacccgttct aaagccatcc
    13021 tggctgcagc gggtatcgct gaagatgtta agatcagtga gctgtctgaa ggacaaatcg
    13081 acacgctgcg tgacgaagtt gccaaatttg tcgttgaagg tgatctgcgc cgtgaaatca
    13141 gcatgagcat caagcgcctg atggatcttg gttgctatcg cggtttgcgt catcgtcgtg
    13201 gtctcccggt tcgcggtcag cgtaccaaga ccaacgcacg tacccgtaag ggtccgcgca
    13261 aaccgatcaa gaaataatcg gggtgattga ataatggcaa aggcaccaat tcgtgcacgt
    13321 aaacgtgtaa gaaaacaagt ctctgacggc gtggctcata tccatgcttc tttcaacaac
    13381 accatcgtga ctatcactga tcgtcagggt aacgcgttgg gttgggcaac agccggtggt
    13441 tccggtttcc gtggttctcg caaatccact ccgtttgcag ctcaggttgc agcagagcgt
    13501 tgcgctgacg ccgtgaaaga atacggcatc aagaatctgg aagttatggt taaaggtccg
    13561 ggtccaggcc gcgaatctac tattcgtgct ctgaacgccg caggtttccg catcactaac
    13621 attactgatg tgactccgat ccctcataac ggttgtcgtc cgccgaaaaa acgtcgcgta
    13681 taacgcttcg ttttccaggt ttgttggaga aagaaaatgg caagatattt gggtcctaag
    13741 ctcaagctga gccgtcgtga gggcaccgac ttattcctta agtctggcgt tcgcgcgatc
    13801 gataccaagt gtaaaattga acaagctcct ggccagcacg gtgcgcgtaa accgcgtctg
    13861 tctgactatg gtgtgcagtt gcgtgaaaag caaaaagttc gccgtatcta tggtgtgctg
    13921 gagcgtcagt tccgtaacta ctacaaagaa gcagcacgtc tgaaaggcaa caccggtgaa
    13981 aacctgttgg ctctgctgga aggtcgtctg gacaacgttg tataccgtat gggcttcggt
    14041 gccactcgtg cagaagcacg tcagctggtt agccataaag caattatggt aaacggtcgt
    14101 gttgttaaca tcgcttctta tcaggttagt ccgaatgacg ttgtaagcat tcgtgagaaa
    14161 gcgaagaagc agtctcgcgt gaaagccgct ctggagctgg ctgagcagcg tgaaaagcca
    14221 acctggctgg aagttgatgc tggcaagatg gaaggtacgt ttaagcgtaa gccggagcgt
    14281 tctgatctgt ctgcggacat taacgaacac ctgatcgtcg agctttactc caagtaaagc
    14341 ttagtaccaa agagaggaca caatgcaggg ttctgtgaca gagtttctaa aaccgcgcct
    14401 ggttgatatc gagcaagtga gttcgacgca cgccaaggtg acccttgagc ctttagagcg
    14461 tggctttggc catactctgg gtaacgcact gcgccgtatt ctgctctcat cgatgccggg
    14521 ttgcgcggtg accgaggttg agattgatgg tgtactacat gagtacagca ccaaagaagg
    14581 cgttcaggaa gatatcctgg aaatcctgct caacctgaaa gggctggcgg tgagagttca
    14641 gggcaaagat gaagttattc ttaccttgaa taaatctggc attggccctg tgactgcagc
    14701 cgatatcacc cacgacggtg atgtcgaaat cgtcaagccg cagcacgtga tctgccacct
    14761 gaccgatgag aacgcgtcta ttagcatgcg tatcaaagtt cagcgcggtc gtggttatgt
    14821 gccggcttct acccgaattc attcggaaga agatgagcgc ccaatcggcc gtctgctggt
    14881 cgacgcatgc tacagccctg tggagcgtat tgcctacaat gttgaagcag cgcgtgtaga
    14941 acagcgtacc gacctggaca agctggtcat cgaaatggaa accaacggca caatcgatcc
    15001 tgaagaggcg attcgtcgtg cggcaaccat tctggctgaa caactggaag ctttcgttga
    15061 cttacgtgat gtacgtcagc ctgaagtgaa agaagagaaa ccagagttcg atccgatcct
    15121 gctgcgccct gttgacgatc tggaattgac tgtccgctct gctaactgcc ttaaagcaga
    15181 agctatccac tatatcggtg atctggtaca gcgtaccgag gttgagctcc ttaaaacgcc
    15241 taaccttggt aaaaaatctc ttactgagat taaagacgtg ctggcttccc gtggactgtc
    15301 tctgggcatg cgcctggaaa actggccacc ggcaagcatc gctgacgagt aaccggatca
    15361 caggttaagg ttttactgag aaggataagg tcatgcgcca tcgtaagagt ggtcgtcaac
    15421 tgaaccgcaa cagcagccat cgccaggcta tgttccgcaa tatggcaggt tcactggttc
    15481 gtcatgaaat catcaagacg actctgccta aagcgaaaga gctgcgccgc gtagttgagc
    15541 cgctgattac tcttgccaag actgatagcg ttgctaatcg tcgtctggca ttcgcccgta
    15601 ctcgtgataa cgagatcgtg gcaaaactgt ttaacgaact gggcccgcgt ttcgcgagcc
    15661 gtgccggtgg ttacactcgt attctgaagt gtggcttccg tgcaggcgac aacgcgccga
    15721 tggcttacat cgagctggtt gatcgttcag agaaagcaga agctgctgca gagtaatctg
    15781 aagcaacgta aaaaaacccg ccccggcggg tttttttata cccgtagcat ccccacttat
    15841 ctacaatagc tgtactcttt ttgttcatcc cctggagtat atatgtggtt acttgaccag
    15901 tgggcagagc gtcatatagc agaagcgcaa gcgaaaggtg agtttgataa cctggcaggt
    15961 agcggcgaac cattgatact ggctgatgat tctcatgtgc caccggaatt acgtgcgggg
    16021 tatcgcttgc tgaagaatgc cggttgctta ccgccagaac ttgagcagcg gagggaagca
    16081 attcagcttc tggatatcct gagaggtatc cgtcacgatg atccgcaata tcaagaggtt
    16141 agccgtcgat tgtcattact ggaattgaag ctgcgacaag ctggactgag caccgatttt
    16201 ttacgcggcg attatgctga caagttgttg aataaaatca acgataacta gtggagtatg
    16261 tatgtatcgc attggtgagc tggcaaaaat ggcggaagta acacccgaca cgattcgtta
    16321 ttacgaaaaa cagcagatga tggagcatga agtgcgtact gaaggtgggt ttcgcctgta
    16381 taccgaaagc gatctccagc gattgaaatt tatccgccat gccagacaac taggtttcag
    16441 tctagagtcg atccgcgagt tgctgtcgat ccgtatcgat cctgaacacc atacctgtca
    16501 ggagtcaaaa ggcattgtgc aggaaagatt gcaggaagtc gaagcacgga tagccgagtt
    16561 gcagagtatg cagcgttccc tccaacgcct taacgatgcc tgttgtggga ccgcccatag
    16621 cagtgtttat tgctcgattc ttgaagctct tgaacaaggg gcgagtggcg ttaagagtgg
    16681 ctgttgattt tttacactgg caggattaca ctcgcgccgt taaataacca attggagttt
    16741 ttatgagccg atatcagcat actaaagggc agataaagga taatgcgata gaagcattac
    16801 tacatgatcc cttattccga cagcgcgtag agagaaataa gaaagggaaa ggcagttaca
    16861 tgcgaaaagg aaaacatggc aatcggggga actgggaggc cagtggcaag aaagtaaatc
    16921 acttttttac cactggcctt ctgctttcag gcgcttgtta agagcggtta ttctgctctt
    16981 tcagcaaatc acgaatttct gtcagtaata cttcttcttt agttggtgta ggcgcggctg
    17041 ctggttcttc ttttttccga ttcagtttgt tgattagctt aatcgccata aagatggcaa
    17101 aggctacaat cagaaaatca aagacgtttt gaatgaagac accgtaatgc atcacaacag
    17161 cagggatatc cccctgcgca tcgcgtagcg tgacagcaaa ctgtttgaaa tcgatcccgc
    17221 caattaataa ccccagaggt ggcataatga tatcggcaac cagtgaagat acaatcttcc
    17281 cgaatgccgc accgataatg acacccaccg ccaaatccac cacgttcccg cgcatcgcaa
    17341 attcgcgaaa ttctttaata atgctcatgt tattctccct atgaagccga caatcataag
    17401 tctaacaaat gttaagctat tttcctgcca ggagtgaatt taattaatcc ctgataatca
    17461 aagggaaata ataaggcgtc attagacgcc ttattaatta caagaagaaa ggacttggct
    17521 ggaagagtct ttcgacgtcg gtaataaact ttttatctgt gaggaacata attacgtgat
    17581 cgccttgctc aatgcgcaga ttgtcattgg caatcatcac gtcgtttcca cgtaccaccg
    17641 ctccaataat cgttcctggc ggtagcttga tttcatcaat gactctgccg acaacgcgtg
    17701 aggtgctttc atcaccgtga gcaacggctt caatagcttc tgctacgccg cggcgcagtg
    17761 aggaaacacc aacaatatca gcttttcgca catggctaag caacgcagaa atagttgctt
    17821 gttgcggtga aatcgcaata tcgatcacgc tcccctgaac cagatccaca taagcgcgac
    17881 gctggatcaa taccatcacc tttttcgcac ccatacgttt ggcaagcatg gcggacatga
    17941 tattggcttc gtcatcgttg gtgacagcaa taaacagatc aacttgatcg atatgttctt
    18001 cggccagcag ttcttgatcc gacgcatcac caaaaaagac gatcgtattc tgcaactttt
    18061 ccgccagttc ggcagcgcgc tgctgattac gttcgatgag tttaacgctg taatcttttt
    18121 ccagacgacg cgccagccct gcaccgatat taccgccgcc aaccagcatg atccgcttat
    18181 acggtttttc cagtcgctgt aattcactca tcaccgcgcg gatatgctgt gaagcggcaa
    18241 taaagaacac ttcatcacca gcttcaacaa tggtcgaacc ttgcggacga atggggcgat
    18301 cgtggcggaa aattgctgcc acacgagtat cgatatgggg catatgttcg cgcatggtcg
    18361 aaagtgcatt accaatcagc gggccgccat aataggcttt aaccacagcc aggctgactt
    18421 taccctcagc gaagttcacc acctgcaatg cgccgggata ctcaatcagt cggtaaatat
    18481 tatcgataac caactgctct ggtgcgatca gatgatcaat cggtacagca tctgaatgaa
    18541 atagtttatc ggcatcgcgc acgtagtctg gtgagcggat acgagcgatg cgattagggg
    18601 tgttgaaaag tgagtaggct acctggcagg caaccatatt ggtttcatct gaactagtta
    18661 cagcaaccag catatcggcg tcgtcggcac ctgcctcccg caatacgcgt ggatgagagc
    18721 catgcccctg cacgactcgc aggtcaaatt tatcctgcaa ggtccgcaga cgctcaccgt
    18781 tggtatcgac aacagtaata tcgttgttct cgccaaccag gttttccgcc agtgtgccgc
    18841 caacctggcc ggcacccaga atgataattt tcatcagtcg cgacccgtta tctcatcact
    18901 ttttgattag cttagcgtaa aagaagccgt cgccctcttc ggcaccaggc agattttgtt
    18961 tacccggttg ctctggtgtt cctgtttcgc aaagttcggc atcagcggta cgttgcaaaa
    19021 aggctttaat ctgcaggcta ttctcttccg gtaacaccga acaggtggca tagaccaggg
    19081 ttccaccgga ttttaaatgc ggccaaatgg cgtcgagaat ttcagactgc aattgcgcga
    19141 gttccgggat atcgcgatcg cgacgtaacc atttaatatc cggatggcga cgaatcacac
    19201 cggttgctga acaaggcgca tctaataaaa tgcgatcaaa ctgttgctcg ccacaccatt
    19261 gggaagggta gcggccatca ccttgtttca cggttgcctt cataccaagg cgttttaaat
    19321 tgtcgtaaac gcgagagagg cgctgttcgt caatatcaac cgcaacaacc tgcgcttctg
    19381 gtgccacctc aaggatatgc gttgttttac cgccgggggc ggcacaaaga tccaaaatgt
    19441 gttcaccgtt ttgtggcgca agccaggtca tgcaaccttg tgctgaggca tcctgaacgg
    19501 taacccatcc gtcttcaaaa ccaggtagcg catgaacagg tgcaggtgtt tccagacgta
    19561 cagcatcagg gtaatccgca ggcgggaaac ctttcattcc tgcttcatcc agcaatgcaa
    19621 gccagctgtc gcgggaatga tgcgtacgat taacgcgcag ccacattggc ggacgctggt
    19681 tattggcttc gacgatggat tgccactgct ctggatacgc tttttgcaga cgcttcagca
    19741 accaggaagg atgcagataa cgtgcatcac tggcattaaa ctcggctaat aactcttctt
    19801 gctgacgctg gaactggcgt aatacaccgt ttatcaaccc tttaagttgc gggcgcttaa
    19861 ttgcgatagc gccttcaacc gtttcagcca gcgcagcatg aggtggtatg cgggtataaa
    19921 gcagttgata caaaccaacc ataatcaggt aatgcacggt ccgctgtttg ccggtcatcg
    19981 gacgggccat taacttatta atcagccagt ttaactgcga aagcgtacgc agtacgccaa
    20041 agcacaactc ttgaagaagt gctttgtctt tatcggaaac tttttgctgg agcggtggca
    20101 gaatgttgct taatgattgc ccttgctcga cgacttgttc aacggcctgg gccgccatgc
    20161 tacgtaaatt acgttgtttt ttcataagta taaaaatgcc cggcaagacc gggcttagaa
    20221 gagtggacta tcagaccaga cggttgcccg gaacaaacca ttcccgacga gagttcagga
    20281 ggtcttgcgc gctcatcgct ttcttacccg caggttgtaa cgagagcagg ttcaggatgc
    20341 catcaccagt cgcaacctga atgccttgtt tgttggcttc aaggatcgtt cctggtgcag
    20401 cgttggttgc cgtatcaatg accgatgctt tccagacttt aaccggctgt ccttcaattt
    20461 ccagccagct cattggccat ggattgaaag cgcgaatgca gcgttcaagc tgtgctgccg
    20521 aaagtgacca gtcaatacgc gcttcttctt tactcaactt ctcggcgtaa gtgacaagag
    20581 tttcgtcctg aacttctggt ttcgccgtgc cgtctgccaa ttgtttcaac gtggtgataa
    20641 gcccttgtgg gccaagctct gccagcttgt cgtacagcgt accactggta tcttctgcag
    20701 taatcgggca ggagagctta tagagcatgt ctccggtgtc taaaccaaca tccatttgca
    20761 taatggtcac accagtttct gcatcacccg cccatagtga gcgttggatt ggtgcagcac
    20821 cgcgccagcg tggcagcagt gaaccatgaa cgttgataca gccaagacgc ggcatctcca
    20881 gcactgcttt aggcagaatt aagccatagg cgacgacaac cataacatca gcctgtagat
    20941 cggcgaccag ttgctggttt tcttgtggac gcagggaaac cggttgaaaa acgggcagac
    21001 ctttttcctc agccagaact ttaaccgggc tgggcatcag ttttttaccg cgtcctgccg
    21061 gtcggtctgg ctgggtgaac acgccaacga cgttatgacc agaagacaac agcgcgtcga
    21121 gatgacgcgc tgcaaagtca ggtgtacccg caaaaataat acgtagtgat tctgacacgt
    21181 tagttcttat ccttaagccc gggctttcag acgatccagt ttttcaactt tctgacgaat
    21241 acgttgttgt ttcagcggtg acagataatc cataaacagt ttgccgacca ggtgatccat
    21301 ctcatgctga atacagatgg ctaacagacc ctctgcttcc agttcaaatg gtttaccgtc
    21361 gcggtccagg gcgcgaattt taactttctc tgcgcgtggc actaaagcac gttgttcagg
    21421 gatcgacagg catccttctt caatgcctgt ttcgccgctt ttttctaaaa gctccggatt
    21481 gattaacacc agccgttcgt cacggttttc cgaaacatca ataacaatga tacgttgatg
    21541 gatatcgacc tgggttgccg ccaggccaat accttcttct gcgtacatcg tctcgaacat
    21601 atcatcgacg atacgctgaa tttctgcatt cacttcttct accggtttag caactttgcg
    21661 aagccgctcg tccggaatat gtaacacttg caaaactgac ataaatctcc agagatgtgt
    21721 tcaggagtta gaaagattat ttcttctatt ctagacaaat ccccctctga ttgacagcat
    21781 cactgaccaa tcgcaaagat tgctaaggct gcttatggca gggagataag gatggtcgat
    21841 acagaaattt ggctgcgttt aatgagcatc agcagcttgt acggcgatga tatggtccgt
    21901 atagctcact ggctggcaaa acagtcgcat attgatgcgg ttgtattgca gcaaactggg
    21961 cttacattgc ggcaggcaca acgctttctt tcatttccgc gaaagagtat cgaaagctca
    22021 ctttcttggt tggagcaacc caaccatcat ttaattcctg cggacagcga attttatcct
    22081 cctcaacttc ttgcgacgac agattacccc ggcgcactgt ttgttgaagg agaactgcac
    22141 gcgctgcatt catttcagct tgccgtagtg gggagtcggg cgcattcatg gtatggcgag
    22201 cgatggggac gattattttg cgaaactctg gcgacgcgtg gagtgacaat tacgagtgga
    22261 ctggcgcgtg gaatcgatgg tgtggcgcat aaagcggcct tacaggtaaa tggcgtcagc
    22321 attgctgtat tggggaatgg acttaatacc attcatccgc gtcgccatgc ccgactggct
    22381 accagtttgc ttgaacatgg tggggctctt gtctcggaat ttcccctcga tgttccaccc
    22441 cttgcttaca atttcccacg aagaaatcgc attatcagtg gtctaagtaa aggtgtactg
    22501 gtggtggaag cggctttgcg cagtggttcg ctggtgacag cacgttgtgc gcttgagcag
    22561 gggcgtgaag tttttgcctt gccgggtcca ttagggaatc cgggaagcga agggcctcac
    22621 tggttaataa aacaaggtgc gattcttgtg acggaaccgg aagaaattct ggaaaacttg
    22681 caatttggat tgcactggtt gccagacgcc cctgaaaatt cattttattc accagatcag
    22741 gaagacgtgg cattgccatt tcctgagctc ctggctaacg taggagatga ggtaacacct
    22801 gttgacgtcg tcgctgaacg tgccggccaa cctgtgccag aggtagtaac tcaactactc
    22861 gaactggagt tagcaggatg gatcgcagct gtacccggcg gctatgtccg attgaggagg
    22921 gcatgccatg ttcgacgtac taatgtattt gtttgaaacc tatattcaca cagaagctga
    22981 gttgcgtgtg gatcaagaca aacttgaaca ggatcttacc gacgcaggat ttgatcgaga
    23041 agatatctac aatgccctgc tttggctgga aaagcttgcg gattatcagg aaggactggc
    23101 agaaccgatg caactggcct ctgatccact ctccatgcgt atttataccc ctgaagagtg
    23161 tgaaaggctg gatgccagct gtcgtggttt tctgcttttc cttgagcaaa ttcaggtgct
    23221 taaccttgaa acccgtgaaa tggtgataga gcgagtgctc gcgctggata ccgcagagtt
    23281 cgacctggaa gacctgaaat gggtaatcct gatggtgttg ttcaatattc cgggctgcga
    23341 aaatgcgtac cagcaaatgg aagaattact ctttgaagtg aatgaaggta tgctgcatta
    23401 atcatttctt taattcagca taagttgtta tggcgaaatc agcactgttc acggtgcgta
    23461 ataatgagtc ctgcccaaag tgcggggctg aactggttat tcgatccggg aaacacggtc
    23521 cgtttcttgg atgctcacag tatccggcgt gtgactacgt ccgtcctctg aaatcttcag
    23581 cggatggaca tattgtcaaa gttctggagg ggcaggtttg ccctgcatgt ggcgcaaatc
    23641 tggtattacg ccagggacgc tttggtatgt ttattggttg cagtaactac cctgaatgcg
    23701 aacataccga acttatcgat aaaccggacg aaacagcaat tacctgtccc caatgtcgga
    23761 cgggccatct ggtccagcgc cgttcccgtt atggcaaaac atttcactcc tgtgatcgct
    23821 acccggagtg tcaatttgcc attaacttca aacccatagc tggagaatgc cctgagtgtc
    23881 attatccgct actcatcgaa aagaaaaccg cgcagggtgt aaaacacttt tgtgccagta
    23941 aacaatgtgg aaagccggtt tcggcggaat aataacgtga ataataacct gcaaggagac
    24001 gctatcgcag ctgcgataga tgtcctcaat gaaaaacgtg tcatcgccta tccaacggaa
    24061 gccgttttcg gtgtcgggtg cgatcctgat agcgaaacag cagtgatgcg actgttggag
    24121 ttaaaacagc gtccggttga taaggggctg attttaatcg cggcaaatta cgagcagctt
    24181 aaaccctata ttgatgacac catgctgact gatgcgcagc gtgaaaccat tttttcccgc
    24241 tggccaggtc ctgtcacctt tgtctttccc gcgcctgcga caacaccgcg ctggttgacg
    24301 ggccgctttg attcgcttgc tgtacgagtc accgaccatc cgttggtggt tgctttgtgc
    24361 caggcttatg gtaaaccgct ggtttctacc agtgccaact tgagtggatt gccaccttgt
    24421 cgaacagtag acgaagttcg cgcacaattt ggcgcggcgt tcccggttgt gcctggtgaa
    24481 acgggggggc gtttaaatcc ttcagaaatc cgcgatgccc tgacgggtga actgtttcga
    24541 caggggtaac ataatggaaa cctatgctgt ttttggtaat ccgatagccc acagcaaatc
    24601 gccattcatt catcagcaat ttgctcagca actgaatatt gaacatccct atgggcgcgt
    24661 gttggcaccc atcaatgatt tcatcaacac actgaacgct ttctttagtg ctggtggtaa
    24721 aggtgcgaat gtgacggtac cttttaaaga agaggctttt gccagagcgg atgagcttac
    24781 tgaacgggca gcgttggctg gtgctgttaa taccctcaag cggttagaag atggacgcct
    24841 gctgggtgac aataccgatg gtataggttt gttaagcgat ctggaacgtc tgtcttttat
    24901 ccgtcctggt ttacgtattc tgctcatcgg cgctggtgga gcttctcgcg gcgtactact
    24961 gccacttctt tccctggact gtgcggtgac aatcactaat cggacggtat cccgcgcgga
    25021 agagttggct aaattgtttg cgcacactgg cagtattcag gcgttgggta tggacgaact
    25081 ggaaggtcat gagtttgatc tcattattaa tgcaacatcc agtggcatca gtggtgatat
    25141 tccggcgatc ccgtcatcgc ttattcatcc aggcatttat tgctatgaca tgttctatca
    25201 gaaaggaaaa actccttttc tggcatggtg tgagcagcga ggctcaaagc gtaatgctga
    25261 tggtttagga atgctggtgg cacaggcggc tcatgccttc cttctttggc atggcgttct
    25321 gcctgatgta gaaccggtca tcaaactatt gcaacaggaa ttgtcagcgt gaatcaggcc
    25381 atccagtttc cggataggga agagtgggac gagaataaaa gatgtgtatg ttttcccgct
    25441 ctcgtgaatg gtatgcaact gacatgtgcg atctctggcg agagtctggc gtatcgcttt
    25501 actggagata cgccagaaca gtggttagcg agttttcgtc agcatcgctg ggacctggaa
    25561 gaagaagcgg aaaacttaat tcaggaacaa agtgaagatg atcaaggttg ggtctggtta
    25621 ccctgatcca gatattcgtc cttccatttc acgtaattct tcgcggaata gcgtaaccca
    25681 gccttctctt catcacttaa cgggcggatc tgtttgacgg ggctaccgag atacagatat
    25741 ccgctctcca gccgtttatt ttgtgggacc aggctacccg caccaatcat cacatcatct
    25801 tctactattg cgccatcaag taaaattgag cccatcccaa ccaaaactcg attgccaatg
    25861 gtgcagccgt ggagcatcac cttgtgacca acagtgacat cttcgccaat ggttaatggg
    25921 ttgccatctg ggttgtacga ggatttatga gtgacatgca acatactgcc atcctggata
    25981 ttggtgcgtg ctccgatctg tacataatgt acatctccac gaatcacaac gagcggccag
    26041 atccccacat catcagccag acgaacgtca ccaatcacga cgctgctatc gtcgatcatt
    26101 acgcgctggc cgatttgtgg aaaaagatcg cggtatgggc gtaaaacatc agacatactc
    26161 acctcaacaa taaatgattt actaatgact ttgggtgcat tattggcctt gtgcaagtct
    26221 tttagcatgc aaaaaagcac cgttttgtgt gcgaatgcag caaaaagggt gaaaaagcaa
    26281 caaacagaaa aaaagatcaa aaaagtactt gtgcaaaaaa ttgggatccc tataatgcgc
    26341 ctccgttgag acgacaacgt gaaacacttc acaagatggt cgtaacaaca aagagaaaaa
    26401 atcctgaaat tcggggttga ctctgaaaga ggaaagcgta atatacgcca cctcgcgaca
    26461 gtgagctgaa agccgcgtcg c
//
LOCUS       DAZFLD010000051        26406 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.051, whole genome shotgun sequence.
ACCESSION   DAZFLD010000051 DAZFLD010000000
VERSION     DAZFLD010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26406
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.051"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(404..1015)
                     /locus_tag="ACHKBL_001780"
     CDS             complement(404..1015)
                     /locus_tag="ACHKBL_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000356295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO5818660.1"
                     /translation="MDKAEKTVSSAERFIDMEGINRFSERLAMAMGNMTNVELATLCG
                     LSEATVRNYRKGKNFPPLDKLALIAKACNCSLEWLATGKNEDECGKIENNCDSGSGID
                     QALSSLSPQEKQLILTFIRREGTNNLVRLAAMNTSTISQDAVESIIDALPLRPVLKNA
                     IKIGMAGSEETDKEILRVIEKYNSSNNATQESGAVTVKKNVVG"
     gene            1172..1438
                     /locus_tag="ACHKBL_001781"
     CDS             1172..1438
                     /locus_tag="ACHKBL_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001266279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO5818661.1"
                     /translation="MRVSRQASNNLFVREGCDWSAKAVYAALENKGTDLPELERELGL
                     KSGSMRNVFYRQCRKYEAAIAEKIGVEPAVIWPSRYQVDAREVA"
     gene            1449..3539
                     /locus_tag="ACHKBL_001782"
     CDS             1449..3539
                     /locus_tag="ACHKBL_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mu transposase C-terminal domain-containing
                     protein"
                     /protein_id="HFO5818662.1"
                     /translation="MWVTAKECVGLPDLPMMEHNIRHRLTKAAAGNAAMLRKRKGTKA
                     IEFNIDCLPSGAQQVLRERYALQLMTQKADESPAPVVTKARRSPAVVDAVEAYRGSPQ
                     LMVERLNALTENQRQVADARIAIVSEVMKVAQQPGFSCAKAIRFIVDNLARSQLDERI
                     VAMVETANAKKGNSRALSEITLKRWIAAFNKAQNAAERLLLLAPGKRQEIKAEDINWL
                     PEFLAQYRQSNGRPMTEAYEDFVAEWQHRHADEPYMLDIMPSYDTIRRAMKKLPEVVK
                     QKGRVTGSEYRQLEGFTRRDWSRMPVNYVWIGDGHGMKLKCAHPVHGRPFAPEVTFVI
                     DGGTRFVVGWSLDLAENVFAVAGAIQHGIRHHGKPFLYYSDNGSGETADILDKEVVGI
                     LPRLGINHPTGIAGNPQGRGIIERLNRTLPMRIARKYRTYFGKGADRETLRKTNRDLR
                     SAFTALQQGKRLNARQQSAMRDLPSWSELIDAIRDGVEWYNNRPHDELPVKPNGKHYS
                     PAEFRKKRLAEEDTEIEWLSDVELRDMFRPMVERPVRRCEIRWLNNIYYAPELRDEHG
                     RKVLISYDIHDAERITVRRLDGSVICEAVWDGNKREAFPVSAEYYKQQQRLKGMRKRA
                     EEKIRDAEDEVVNVLEHKPQEPWLENIYRPVGNTVTVQQPVADDEPDEEYERNFQRGL
                     QLLEAKLKENDPLA"
     gene            3611..4543
                     /locus_tag="ACHKBL_001783"
     CDS             3611..4543
                     /locus_tag="ACHKBL_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFO5818663.1"
                     /translation="MTDINDVIKTIDELIDGGVLTQYAIAREAGISDGTLSAFRKGKY
                     KGDNAAVAASLRSWYENWNKQSALPEPPQFVETQTVQELRALFQAVRLMGCINVIVGV
                     PGVGKTATARNYCQEQPNTWMITLSPAHSSVTECLLELADALGIDYTRANKGALSRAI
                     RRRLMGTRGLVIVDEADHLGIDGLEQLRAIQDATGIGMVLIGNPRGLFKGGRRTFDDL
                     SRLFSRLARTKQLRKAKKADVLAIARAWGISGEAELAVMQAIAEKPGALRVLTHTLNQ
                     AWLTASGEGAALTEKHINAAFKEVYTNPELLSQV"
     gene            4546..4767
                     /locus_tag="ACHKBL_001784"
     CDS             4546..4767
                     /locus_tag="ACHKBL_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5818664.1"
                     /translation="MAVFNIPDIYGRFYLVNFDNVKVISLAENKECGDLLFEFNDRTR
                     MVISAGLDREGATDVYSGICRSVGAKQVS"
     gene            4780..5034
                     /locus_tag="ACHKBL_001785"
     CDS             4780..5034
                     /locus_tag="ACHKBL_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5818665.1"
                     /translation="MNMQSCGNKMNLFDSLNSARRLTELAGAVLERSKRYPQRFALKT
                     TPPVGNVQGTGEIEITIQTNGLRRRVKATRISGCTVYWEV"
     gene            5036..5317
                     /locus_tag="ACHKBL_001786"
     CDS             5036..5317
                     /locus_tag="ACHKBL_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host nuclease inhibitor protein"
                     /protein_id="HFO5818666.1"
                     /translation="MKKNLIAWAWSSGLIEFGYVLPEGALPIVAGKPATVRHVIEVMA
                     RHGRDEQEQLLVPGIPEAVTEEEAFNAMIRFCREVRRRVSYPNRTRTRG"
     gene            5314..5586
                     /locus_tag="ACHKBL_001787"
     CDS             5314..5586
                     /locus_tag="ACHKBL_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5818667.1"
                     /translation="MSKVVRIIFEYKEHVIHKNADGTVRMGVSLDIRSTGIKQKGDGP
                     AMIFGVVMLAESRDFAELVAMKASALMKDMSMRSGVIKGNEFNQQG"
     gene            5591..5884
                     /locus_tag="ACHKBL_001788"
     CDS             5591..5884
                     /locus_tag="ACHKBL_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5818668.1"
                     /translation="MSKVRVIFEFKHVSHDEKPAGNDCVEVHEKIGVDVKTERDTNNR
                     PTSLCDVYASILQYHSPEIIQFLSAEFQASVQAFGADAIIKRHRVHKASGTLQ"
     gene            5896..6426
                     /locus_tag="ACHKBL_001789"
     CDS             5896..6426
                     /locus_tag="ACHKBL_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor Gam family protein"
                     /protein_id="HFO5818669.1"
                     /translation="MAKRVTKLKAAAEAAPQTREEVSRDIRTLGDIQREALRLETAMN
                     DEVAEITARYTPQIENLKKEIKVLFKGIQDWCKTNRDELTNGGKTKTANLTTGTVSWR
                     LGNPSCSVSRDVEGVIEMLRRMGLERFIRTKEEVNKEAVLAEPDAVKGIAGIKVNKGA
                     ESFYVEPFEQDAGLNK"
     gene            6524..7066
                     /locus_tag="ACHKBL_001790"
     CDS             6524..7066
                     /locus_tag="ACHKBL_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5818670.1"
                     /translation="MAYFYFKLDRVQTNKYFTKYQQTVLPLRNSILRALLKNTGAAGL
                     RLKPFAMDVISEFYFSGALPAGWRKRDDVAFVGDGPCFIARPDESCPEGPAIAAMIEA
                     AERELRKRPDFLVWLCEKLGVMRIPSMFNMDSWWTPSLSRDALCVVFKVGAYGREIKG
                     GIPEECQEIKHSEYVALTEE"
     gene            7070..7603
                     /locus_tag="ACHKBL_001791"
     CDS             7070..7603
                     /locus_tag="ACHKBL_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5818671.1"
                     /translation="MIDAKVLEGVKNWLSIYGRLTCGILAEKMNMPPSSMVYFLRDAV
                     DAGVLTECNGFYDIPRPRPVQPVRRKCSQEGAADDVQWCSFRKSLPWIEGHDIPSMAW
                     EFAQGVLTCETVYVVAEVDEQAMKEGVPQFVMAYIDIRLGVIICGLSGWNITSRVLRY
                     LIVDRTAAPAGISAEVA"
     gene            7603..8118
                     /locus_tag="ACHKBL_001792"
     CDS             7603..8118
                     /locus_tag="ACHKBL_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5818672.1"
                     /translation="MFFKTSNPSALAAWQKYQQDCQKVKDEAKRLEAVLNVACRSVFV
                     SGISGFCFKGLRFMDDKYPFHRDLWRKPTASNGWSCTPRTSRIPKALRVASDELNSLW
                     REYSPVTYARTDALLFWLGIDFSAIMFGPVKWFCVDDVIYLECGVKPANQKMTEILSD
                     EFYAAEKRVRG"
     gene            8122..8673
                     /locus_tag="ACHKBL_001793"
     CDS             8122..8673
                     /locus_tag="ACHKBL_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000973023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsnC family protein"
                     /protein_id="HFO5818673.1"
                     /translation="MMILQPMGRKGQAPAHVRAWTPEEDALLIALYPSTPVKDIAVRV
                     KRSFWGVHNRIVLLRGTYPELLKCKRPRFKHDEDKFIRKNARTMTGKQIGEYLGRDRD
                     SVHNRARYIGVSMKKYGELLPFTRIPDDDVHLIRELRDAESPRRLTFREIGEKFELSE
                     STVNFIYHHRRTAEDAVLRELTP"
     gene            8670..8855
                     /locus_tag="ACHKBL_001794"
     CDS             8670..8855
                     /locus_tag="ACHKBL_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5818674.1"
                     /translation="MIKRLVFFAIFVPVLGVGFIFAITGNLSMMPDIWFFIRMSLFLF
                     IMNLLIDIYIRITGKHK"
     gene            8894..9226
                     /locus_tag="ACHKBL_001795"
     CDS             8894..9226
                     /locus_tag="ACHKBL_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5818675.1"
                     /translation="MSIELRSSYEYRKFLIAGGMKPEDVEKIVSFMDKECDKRDVSEI
                     IMDDMILDSVVALSPLWIVHSLAEIAKGTDKQAAVAALQTLNEMRISPRPTLIQMILS
                     SMEDKANE"
     gene            9219..9416
                     /locus_tag="ACHKBL_001796"
     CDS             9219..9416
                     /locus_tag="ACHKBL_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5818676.1"
                     /translation="MNSLPAGWARPLMAKKHHFFKTGDNISICRRWLYLAHNREPDTF
                     ESPDDCAECRRRLNKEKDNGQ"
     gene            9406..9702
                     /locus_tag="ACHKBL_001797"
     CDS             9406..9702
                     /locus_tag="ACHKBL_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5818677.1"
                     /translation="MDNSSLLFWCLYITSFFGAFVITRWLCRKIICFFDKRHPVERAA
                     DALIQQAIVLYSGEFFCRITTRDGWHIMIIPPTHHARWDEAEKAFHVRKKVNTV"
     gene            9699..10208
                     /locus_tag="ACHKBL_001798"
     CDS             9699..10208
                     /locus_tag="ACHKBL_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gp16 family protein"
                     /protein_id="HFO5818678.1"
                     /translation="MRGKLISAIHVAKRELALDDETYTSALLAATGKTSCRDMSPDEL
                     SRVLDVFKKRGFKVRQNPVNRALKPGTVTAKIRAIWKVMHRQGFISDGAETALNRWVK
                     SQTAAQNGGEGVANWQWLEQHPALASDVLERLKRWHRRKMLAAMGMPERTLMGYDAVC
                     RQYEKSLPR"
     gene            10278..10703
                     /locus_tag="ACHKBL_001799"
     CDS             10278..10703
                     /locus_tag="ACHKBL_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mor transcription activator family protein"
                     /protein_id="HFO5818679.1"
                     /translation="MAETQMSMFGGDSEQLHALIDRLDDIPDDVLKKNWPRTLSELVE
                     VTGAELQRQGIEPVLAGKLARKVAAAQAAYMGGRGYYLPVGESLFAELRNNEIFSRWD
                     RGEKIESLRRHYRMSETQIYTVIREQRRLHLARTQPPLF"
     gene            10775..11275
                     /locus_tag="ACHKBL_001800"
     CDS             10775..11275
                     /locus_tag="ACHKBL_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFO5818680.1"
                     /translation="MPKLPAPLRKKLIALVLAGAGTFTIATHYTGYWEGKENSTYIDP
                     TGTPTICYGHTGPDVKPGMTLTDEECLELLEKDMKWAFAAIDRRVQVPLTRGQTVALA
                     SWIFWAGETNFRNSTLLRLINAGQMPASCKQYIRWIYSKGVKLPGLEARRSADEWLCR
                     YDLPKV"
     gene            11310..11738
                     /locus_tag="ACHKBL_001801"
     CDS             11310..11738
                     /locus_tag="ACHKBL_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5818681.1"
                     /translation="MCGTAVTGCPVAAVNNALPALIAGACMAALGIIAVLVAAIWGMH
                     QKTQRLEDNNQVLVRERDEARQVLANQQHTLQLISQISEAATNEKQQNIQHSEGQQSV
                     VRRSLAAVPAASAPVPGDVADRVRRAVCEIRACEAGADPR"
     gene            11722..11940
                     /locus_tag="ACHKBL_001802"
     CDS             11722..11940
                     /locus_tag="ACHKBL_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001122253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5818682.1"
                     /translation="MPIPAEWLADCLVPPAPEPFTFGASVTYNLQLLAVIKNCNVDKA
                     SIRRLEARRQHEFTDMAGTPAVPAGKTK"
     gene            11951..12178
                     /locus_tag="ACHKBL_001803"
     CDS             11951..12178
                     /locus_tag="ACHKBL_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HFO5818683.1"
                     /translation="MDDSDRAQAVMERGSERALCNRLTRKRRAMDAPGRRVCADCGGE
                     IPAARLVAVPDAIRCVNCQNIMEARHVGQHR"
     gene            12159..12467
                     /locus_tag="ACHKBL_001804"
     CDS             12159..12467
                     /locus_tag="ACHKBL_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2730 family protein"
                     /protein_id="HFO5818684.1"
                     /translation="MWVSIVKDYVVPILSATATAGGIFMALMRKTFVPREAFEKLSDR
                     VEKVETRLSSLPTEAEVNRLNVEIVTLRGELKTTNATLRSVSYQNELLLEQAVRKKTQ
                     "
     gene            12464..12754
                     /locus_tag="ACHKBL_001805"
     CDS             12464..12754
                     /locus_tag="ACHKBL_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HFO5818685.1"
                     /translation="MSDFITEDQRLVILRSLADYNGELGESVLQDCLDDYGHRVSRDT
                     VHTHIAWLAEQGLVRKRTLVNGYFIAELTGRGQDVAEGRATVPGVKKPRARG"
     gene            12757..13338
                     /locus_tag="ACHKBL_001806"
     CDS             12757..13338
                     /locus_tag="ACHKBL_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000360582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage protein Gp27 family protein"
                     /protein_id="HFO5818686.1"
                     /translation="MDKPTRGRVRKVDLLPDSIRKPLLEMLREKRLTQVQIREEINRL
                     IREAGLPEEQQLSPAAISREASRNELIARNLRDLREQTKAMMAELGDKPTGETTALIL
                     EMSRALMYRRLRAATESLNSDSDVDMRLIKDILLSAQRAESAAERSIKREKEIRAAFA
                     EEMANAVTDELRGVDGMSEQLESRIKGILLGKV"
     gene            13338..15002
                     /locus_tag="ACHKBL_001807"
     CDS             13338..15002
                     /locus_tag="ACHKBL_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5818687.1"
                     /translation="MNNAEELSPLLTNTVSTRKIDLAGEKALLGVDVPDSLDLPGDMP
                     VFLDYQARWFEDESEVCIAEKSRRTGLTWAEAGRNVITAAKPKRRGGRNVFYVGSKQE
                     MALEYISACALFSRAFNQLADADVYEQTFWDRDKKEEILTYMIRFPNSGFKIQALSSR
                     PSNLRGLQGDVVIDEAAFHESLDELLKAAMALTMWGARVRIISTHNGVDNLFNQYIQE
                     AREGRKDYSVHRITLDDAIADGLYRRICYVTGREWSPESEQKWRDDLYKNAPTREDAD
                     EEYGCIPKKSGGAYIPHALIEMAMLRDIPILTFEAPDDFISRAAWLRESEVLTWCEEH
                     LKPLLEALNPRSRFSFGEDFARTGDLSCFVLLEITESLAKREVFRVELRNLPYAQQEQ
                     VMMYILTRVPALVGAAFDATGNGGYLAEAALLAFGPDIIDCVMLSPKWYGEWMPKLKA
                     EFEDQNILVARHQTTLDDLRHVKVVNGIPQIDKGRTKDQNATAVNARRHGDFAVALCM
                     ANRASYMEGFILDESACQALPERSRAMEGGYRDDDEAYHEFDRGCW"
     gene            15002..16591
                     /locus_tag="ACHKBL_001808"
     CDS             15002..16591
                     /locus_tag="ACHKBL_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312997.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF935 family protein"
                     /protein_id="HFO5818688.1"
                     /translation="MGRIIDLDGKPFSFDPEMQSAALDIPQIASRYIEHPASGITPNR
                     AAQCLRGAERGDLIAQSDLAADIEEKDTHLFAELGKRRLAIQGVPWSIEPPPNASANE
                     KKDAEMLDEYLHSADWFDAMLFDATDAILKGYSCMEIEHGMLGKMHIIRAIRWRDSGH
                     FCLNPDDLSELRLRDGSHAGVAFQPFGWIVHQSRSRTGYGGATGLVRTLIWPFIFKNY
                     SVRDLAEFLEVYGLPMKVGKYPSGATPEQKSALMRAVMDIGRRTGGIIPAGMSLEFQA
                     AANGQADPFETMISWGERSISKAILGGTLTTEAGDKGARSLGEVHNEVRREIRDSDLR
                     QLAATLNRDLVYPLYALNTAHAIDIRRLPRICFQTKEPGDITKITSAVMQLSTGMDIP
                     DPWVRDQTGIPQPAPGEAIFRVRQSGNEPAQTDKEMPPEKQEKTEQTALSARLPEAKS
                     SPRDELDDMGDAVPARRLQEAIDPVLEPVIDAIRTRGLAEALADLPALYREMDDSRLM
                     TLLSDAMFAAEMKGMLDGTGD"
     gene            16575..17906
                     /locus_tag="ACHKBL_001809"
     CDS             16575..17906
                     /locus_tag="ACHKBL_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor head protein"
                     /protein_id="HFO5818689.1"
                     /translation="MAQGIDLGYAATLPSKEAVAYFRAKGAHISWNWFETDADVHARS
                     FTAARVARLDVLTTLQAEVQRAVDEGISQKAFIRTLTPRLQKLGWWGKQIVVDSAGNA
                     EEVQLGSPRRLALIYNVNTRVAYNAGRYTQMMNNTDTHPFWQYVAVMDSRTRPAHSAL
                     NGLVFRYDDPFWNTHYPPNGWNCRCRVRPLSQARLDAMGLSVSSGQDHLSTRNVEAGV
                     DKQTGEVREMPVTTYSDGTRTMTPDVGWSYNPGSAAFGTDQALIRKLIEVKSPALREM
                     VVQEMNNSPERQLAFRIWAKNIMKTRRGGNDIRTLGFMTESIAQAVESRTGTPPARLL
                     AMSGKNVLHADSMKHQNDGIALTPEDFGRLPAMLARPKAVLWDKRHNNLMYIVENQDG
                     SVQIAVNAPYSLKRQPDKLDVIVNAYRVINMDKLKSDIRGGMLEVLEGDID"
     gene            complement(17908..17987)
                     /locus_tag="ACHKBL_001810"
     tRNA            complement(17908..17987)
                     /locus_tag="ACHKBL_001810"
                     /product="tRNA-OTHER"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
     gene            18028..18501
                     /locus_tag="ACHKBL_001811"
     CDS             18028..18501
                     /locus_tag="ACHKBL_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HFO5818690.1"
                     /translation="MSSIDAAVVVDVTRLQRVFARLQFVGGGKDLARSVASSLLSSSE
                     MAFEQEKEPDGERWHDWSDPYRKWRTRKGYMPGKILTLNGDLARRLTTDYGDTWALIG
                     SNEPYAAIHQWGGLPGMPPGPAAIGARPYMGFDQVAEQEIMDEIRKRFKKATETP"
     gene            18678..19802
                     /locus_tag="ACHKBL_001812"
     CDS             18678..19802
                     /locus_tag="ACHKBL_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage protease"
                     /protein_id="HFO5818691.1"
                     /translation="MKTKNTPKLAYAILNAISLSADGDGDWCQIMPAGRVKARDGRPE
                     KPAEGWLINHAAVERMVSRVVALNQPVKIDYNHQTLIEGHPAPAAGFVMASPENFRFS
                     EERGFEVRPKWNPPALEHLRNNEFPWFSPVIGYDESTGEPVELRMLAITGDPGLTGMN
                     PVAALSADDLYNALNPPLKDTSMNEQLRQLLTALGLTVADGDEFTPELGTAALSALTG
                     IKTRADAHDNLKTQVASLSAELETAKGTPAGGSIDLTKYVPVETYNALRTEYVALSAQ
                     HGSTTLEQVLDKAESEGRIFKSERGYLEQLGGQIGVAALSAQLDARQPVAALTSLQTD
                     TVTVPDKKTATAVLSAEDIAAAKLLGKTEAEFLKMKEEMQ"
     gene            19802..20749
                     /locus_tag="ACHKBL_001813"
     CDS             19802..20749
                     /locus_tag="ACHKBL_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mu-like prophage major head subunit gpT family
                     protein"
                     /protein_id="HFO5818692.1"
                     /translation="MPTPITPAMITALMTGYRSDFQAGMSMAPSQYKKIAMTVPSTSK
                     SNTYGWLGQFPQFREWIGSRVIEKMKAYGYAIVNKTFEGTVAINRDDFEDDNLGIYSP
                     LFQEMGRAAAAQPDELVFAALRDGINAACYDGQNFFDTEHPVYPKVDGSGDAQMVSNM
                     FVAKTGSVGAQADYSGPAWYLLDCSRAIKPLIYQDRRKAELVAQTKVDEGRAFTDNEF
                     VFGASARRNVGYGFWQMAYMMQSPLTLDALWHGWSAMREFTADGGRKLGIKPTHIVVP
                     TSLEKQAVQLLERELFADGNATVSNEMKGKLELVVADYL"
     gene            20793..21146
                     /locus_tag="ACHKBL_001814"
     CDS             20793..21146
                     /locus_tag="ACHKBL_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001002039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HI1506-related protein"
                     /protein_id="HFO5818693.1"
                     /translation="MNEHHTVAATDDSGLQVSGDNSVTVLAEVRCSRSAFRRAGFLFT
                     RGRQQVEVSPEQLARLEAEPCLTVRILQTPADDAGSVAGVVHAVAGTDLAEAGQDTPR
                     KKTGNKAKQAKKARA"
     gene            21143..21562
                     /locus_tag="ACHKBL_001815"
     CDS             21143..21562
                     /locus_tag="ACHKBL_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gp436 family protein"
                     /protein_id="HFO5818694.1"
                     /translation="MNYATETDMRARYREDLLRPLLAVPRSDEPDTRKLNRALTDASA
                     LIDSYLSARYTLPLEVIPAVLVQHCCAIAFYYLCDQRASDQARDRYREALAWLKDVMN
                     GNVPVGVDTNGAAPESGDLPQVQSDAAVFGRNQKGFI"
     gene            21559..22119
                     /locus_tag="ACHKBL_001816"
     CDS             21559..22119
                     /locus_tag="ACHKBL_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1834 family protein"
                     /protein_id="HFO5818695.1"
                     /translation="MITETEQAYIARIREYFGNELVSVDTHPGDWSDSVLRTMLINAP
                     AIYVAWLGAGEGRTRGRLVSHWVFYVIGDMLNGREASRPGLYQIVARLIAVLNGFRTE
                     KTSPLYFEKAVNGYTETQADSGAVMYALYFSCEEMIAPLTDTSSLDDFLRHYETFVEP
                     PGTPPFEAHIDLPGNTTTAVEPPEEL"
     gene            22120..22365
                     /locus_tag="ACHKBL_001817"
     CDS             22120..22365
                     /locus_tag="ACHKBL_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2635 domain-containing protein"
                     /protein_id="HFO5818696.1"
                     /translation="MKTIFIKPAPGRLIRDPDTMRPLAQEGEEKPFTPFWCRRLDDGD
                     VIQAEKAAEEAPAVSADATTATEKPVAQPASDKEKPQ"
     gene            22362..23864
                     /locus_tag="ACHKBL_001818"
     CDS             22362..23864
                     /locus_tag="ACHKBL_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HFO5818697.1"
                     /translation="MINFDGIGNDRRIPLIEVEFNNSMAVTGTPAQRQCVLLFGQARM
                     KENAVDGAGVLDVPVRITRASQATELWGRGSMIALMVAEFIAINPDAELYAIAQGNGT
                     GQATAAAMNLTGTVTRDGIVYACVGGRRYTLPAPKGKKGKELTDELAALINADPDAPF
                     TASSGAGSGDNGAGLKGSLGITARFTGECSVHDVRLNYHDGEATPEGIQVAIAYPKQK
                     AANPDITRSVAGMGDRQYNYVVMPYKDDANLKIISDELLKRWGPAKMSDGVLWLAHTG
                     TFGEVQAFGAKRNDFLATCTSIPKAPEPDYLWAAAVCATCAPSLGADPARPLQTLALP
                     SRMAPAPADRLTREERNSLLWGGIATVSVAAGDVVQIERQVTMYRQNVYGESDPSYLD
                     VETIYTLSWLRYSLRTFITQRFPRHKLADDGTPVRAGQNIVTPEIMKLQLISLGEEWV
                     ELGLVENLDTFKKNLLVERNASDRNRLDVLCTPDLVNQFRFLAAQIRFIL"
     gene            23873..24238
                     /locus_tag="ACHKBL_001819"
     CDS             23873..24238
                     /locus_tag="ACHKBL_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HFO5818698.1"
                     /translation="MSGKQYQGTATIRVNGQEYETLEGATFSPSGFEREVVKGAKVYG
                     YRQKPREATLDCKFPAGGEGSPAADEINNWTAVTIEFVADTGEVHMMTKAWSSEPASL
                     DGGGEISVKFASATSTRVQ"
     gene            24253..24729
                     /locus_tag="ACHKBL_001820"
     CDS             24253..24729
                     /locus_tag="ACHKBL_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HFO5818699.1"
                     /translation="MTTRKKKTAVSEAAVMEAIREALEGADPRTAGLTEQLAKGYVDL
                     LDGLPFGETREYRVTFRELTAKDSIDAEAEAERVVETNNGPMLIASPSLRGVALLRRQ
                     IAAVGDIEGPLSPRQIGQLSERDLSRLMAAVSLLDTALAGKLAADRGRSGAVSGSD"
     gene            24856..>26406
                     /locus_tag="ACHKBL_001821"
     CDS             24856..>26406
                     /locus_tag="ACHKBL_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HFO5818700.1"
                     /translation="MARNLRASLIVDLLGNISAKSRQWSQELGAFSRSGQAGLGGLGN
                     AARRAGQETDVLGSRMQRTLAGVRGSIRHVTSDFDRLQGSITGTIGRISNLYGMLAGG
                     AAVYGFNRGFIRPAAEMETYMIRLNSLYKGDRAKTDDVRRWAIQNAKETTWGLAGVMQ
                     EYTSSLGFGMSDREARNFVTMLQDQGAVGGWSLSDAQGASLQLKQMYARGSIQAADAN
                     ILATYGINAYRVLADRLGVDQKVVRKLGEKGLLGPDSIRLLFQTLAEQARGAQKDAMN
                     SWSGLTAMMGDVWDQFARDVMDSGPFEKLKGNLRGFLSWVDSAKSDGSYHSLAESTAS
                     AMNQGFEYARDAVTGFYQAIRKVRDTLQALRDAGYGDALDRIGQGAQTAAKYLLYMYL
                     ATRALKMARAVGTGIIRPGAALLGYGLSAAAFLTSPFRRSPPAGTPPGTSQGRGQRFI
                     NFLTGVNPAAVQPVLVTNWPTGGLSGTGATDTSSGGRQGRGRRKRGPGRGRPVTPPLP
                     PSPVPPSPS"
BASE COUNT         6316 a         6609 c         7627 g         5854 t
ORIGIN      
        1 gctgcgtacg ctacggggct ggttatccag ccagccgagc agcgacttgt aaccaggtga
       61 atcatttgat accgacaagg gttttagtga aaggaggtga aatcacctgt aagatgctga
      121 aataaaacag gtgatgtata cttcggacgg aaaactcttt ttgataccga ctgctcaaca
      181 aatagtcaaa agatcaaaaa acggcgacac atttgatact gctaatttac ccttaaaatc
      241 ttcttcaaaa catcttcaaa ctgccaaaat gctcacataa tcgatctaac tataatcagt
      301 atgataatca tcatgaatta ctattcaata gcgtgattat cttgggatga gtcatatgat
      361 ctctatatgt atttacagca ccacctcatg atagcgatgg cgcttaacca acaacatttt
      421 tctttactgt cactgcccct gattcctggg tggcgttatt gctcgagttg tacttctcaa
      481 taacgcgcaa aatctctttg tctgtttctt cacttcccgc cattcctatc tttattgcat
      541 tttttagtac gggacgtaat ggcaaggcat caatgatgct ttctaccgca tcctggctga
      601 ttgtagatgt attcattgct gccagcctga caaggttgtt agtaccttct cgcctgatga
      661 atgtaaggat taattgcttc tcttgggggc ttagagagct aagcgcctga tcaatacccg
      721 aacctgaatc gcaattgttt tcgatttttc cgcattcatc ttcgttcttg cctgtggcca
      781 accattcgag tgaacaatta caggctttag ctattaaggc cagtttatcc aaaggaggga
      841 agtttttccc cttcctgtaa tttcttactg tggcttcaga tagtccacaa agggtcgcca
      901 gttcgacgtt tgtcatgttt cccatagcca tagctaagcg ttctgaaaat cggtttattc
      961 cttccatatc aataaaccgt tccgcactgg atacggtttt ttctgcctta tccatataac
     1021 cccctataaa acaaagaaat tatcaacatc gcatttaatt tcgatgtgct cgcaaaaaac
     1081 cgttgatatc gaattatttt gcgattatga tttacctaaa tcgataaccg gttttgatta
     1141 tcggttcagg taaacatgag aggatcacac aatgagggta tccagacaag catcgaataa
     1201 tttgtttgtt cgagaggggt gtgactggtc tgctaaagcg gtatatgcag cccttgaaaa
     1261 caaaggcaca gacctccctg aactggaaag ggagttagga ttaaaaagcg gatcaatgcg
     1321 caatgtgttt taccgccagt gccgtaaata cgaagccgcc atcgccgaaa aaataggggt
     1381 tgaacctgct gtaatctggc ccagcagata ccaggtggac gccagagaag tggcttgatg
     1441 ggggatgtat gtgggtaaca gcaaaagaat gtgttggtct tcctgattta ccaatgatgg
     1501 aacataacat tcgccatcga ctcacaaaag cagctgcggg aaatgcagcc atgttacgca
     1561 aacgtaaggg gacaaaagca atcgaattta atatcgattg tttgccttct ggcgcgcaac
     1621 aagtattgcg tgaacgttat gcccttcaac tgatgacgca aaaagctgat gaatcaccgg
     1681 ctccggtggt gacaaaggcc agacgctcgc ctgccgtggt tgatgcggtg gaggcatatc
     1741 gcggatcacc acaactgatg gttgaacgcc ttaatgccct gactgaaaac cagcgccagg
     1801 tggctgatgc acgaatcgcg atcgtcagcg aagtgatgaa agtcgcgcaa caacccggtt
     1861 tcagctgtgc gaaagctatc cggtttatcg ttgacaacct ggcacgttcg caactggacg
     1921 agcgcattgt ggcaatggtt gagacggcga acgccaaaaa gggaaacagc cgtgcgttga
     1981 gtgaaataac gctgaaacgc tggattgcgg cctttaacaa ggcgcagaac gccgctgaac
     2041 gcctgctttt actggcaccg ggtaagcgcc aggaaataaa agccgaagat attaactggc
     2101 tgcccgaatt tctggcgcag tatcgccagt caaacggccg accaatgacc gaggcttacg
     2161 aggattttgt cgctgaatgg cagcaccggc acgctgatga gccttatatg ctcgatatca
     2221 tgccgtctta tgacaccatt cgccgcgcaa tgaagaaact gccggaagtg gtgaaacaaa
     2281 aaggccgggt gaccggcagt gaataccgcc agcttgaggg attcacgcgc cgcgactggt
     2341 ccagaatgcc ggtgaattat gtctggattg gtgacggtca cggcatgaag ctgaaatgcg
     2401 cacacccggt tcacggtcgg ccatttgcac cggaagtgac ctttgttatc gacggtggca
     2461 cgcgctttgt ggtgggctgg agccttgacc tggctgaaaa tgttttcgcc gtagccggtg
     2521 ccatacagca cggcattcgc catcacggca aaccgtttct gtattactcg gataacggct
     2581 ccggggaaac cgccgacatc ctggataagg aggttgtggg gatactgccg cgactgggga
     2641 ttaatcaccc gaccgggatt gccggtaatc cgcagggacg aggcattatc gaacggctta
     2701 accgcacatt accgatgcgc atagcccgta aataccgcac ctatttcggg aaaggtgcag
     2761 atcgcgagac gttacgcaaa accaaccgcg atttacgctc ggcatttact gccctgcaac
     2821 agggcaaacg gctgaacgcc cggcagcagt cagcgatgcg tgatttaccg tcctggtctg
     2881 aactgattga tgccattcgt gacggtgttg agtggtacaa caaccggccg cacgatgaat
     2941 taccggtgaa gccgaacggc aagcattaca gcccggcgga gttcagaaaa aaacgcctgg
     3001 cggaagagga cacggaaatt gaatggctgt ccgatgttga gctgcgggat atgttccggc
     3061 cgatggtgga acgccctgta agacgctgtg aaatacgctg gctgaataat atttactacg
     3121 cgcccgagct gcgtgacgag catggtcgta aggtgcttat cagctatgac attcatgatg
     3181 ccgaacgaat taccgtacgt cgcctggatg gcagcgtgat ttgcgaggcg gtatgggacg
     3241 gtaataaacg cgaagccttc ccggttagcg cggaatacta caaacagcag caacgcctta
     3301 aaggcatgcg taaacgcgca gaggaaaaaa tccgtgatgc cgaggatgag gttgtcaacg
     3361 tgctggagca caagccgcag gagccctggc tggaaaacat ataccgccct gtgggtaata
     3421 cggtgaccgt tcagcaaccg gtcgctgacg atgaacctga tgaagaatac gagcgtaatt
     3481 tccagcgggg attgcagttg ctggaagcga aattaaaaga aaatgacccg ctggcctgaa
     3541 ataaaaaaat aacccgagcg gcgactcagg ttatttgatt aaacaaggta tcaaatgaga
     3601 ggttaataat atgactgata ttaacgatgt aatcaagacc attgatgaac ttattgatgg
     3661 cggcgtactg acgcagtatg ccatcgccag agaggcggga atttccgacg gcacattatc
     3721 ggctttccgt aagggaaaat ataaaggcga taacgctgct gtggctgctt ccctgcgttc
     3781 ctggtatgag aactggaata aacaaagcgc actgccggaa ccgccgcagt ttgtggaaac
     3841 gcagacagtc caggagctgc gcgcactgtt tcaggcggtt cgtctgatgg gctgtattaa
     3901 cgttattgtg ggcgtaccgg gtgtgggtaa aacggccact gcccgtaatt actgccagga
     3961 gcaaccaaac acctggatga tcaccctgtc acccgcgcac tccagcgtca cggagtgtct
     4021 gctggagctg gccgatgcgc tggggattga ttacacccgc gcgaacaaag gggcattatc
     4081 ccgcgccatc cgccgtcgcc tgatgggaac gcgtggactg gttattgtgg atgaggcgga
     4141 tcatcttggt attgacggtc tggagcaact ccgggcaatt caggacgcca cggggatcgg
     4201 gatggtgctt attggtaacc cgcgcggatt gtttaaaggt ggacgccgca cctttgatga
     4261 tttatcgcgc ctgttcagcc gtcttgcccg tacaaaacaa cttcgcaagg ccaaaaaggc
     4321 ggatgtgctg gccattgcca gggcgtgggg gatcagtggt gaggccgagc tggccgtcat
     4381 gcaggctatc gctgaaaagc cgggagcgtt acgcgttctg acacatacgc ttaaccaggc
     4441 gtggctcacc gccagcggtg aaggcgcggc gctgacagaa aaacatatta atgcggcctt
     4501 taaagaggtt tataccaacc ctgaattact ctcacaggtg tgattatggc ggtatttaat
     4561 attcctgata tttacggacg cttttacttg gttaatttcg ataacgtgaa ggtgatttca
     4621 ctggccgaaa ataaagaatg tggcgattta ctttttgaat ttaatgaccg cacacgaatg
     4681 gtgatatctg ccggacttga tcgagaaggt gcgacagacg tttacagcgg aatatgccgt
     4741 tctgttggtg cgaaacaagt cagctaaatg aggtgttata tgaatatgca atcctgcggt
     4801 aacaaaatga atttattcga ctccctgaac agcgcgcgcc gtctgaccga acttgccggt
     4861 gcggtactgg aacgcagtaa gcgctatccg caacgttttg cactgaaaac cacgccgccg
     4921 gtaggcaacg tgcagggaac cggtgaaatt gaaatcacca tacagaccaa cggcctgcgc
     4981 cgccgtgtga aggccacccg catcagcggc tgcacggttt actgggaggt gtgaggtgaa
     5041 aaaaaatctc attgcatggg cgtggtcgag tggtcttatt gagtttggtt acgtcctgcc
     5101 ggaaggtgca ttgccgatag ttgccggaaa gcctgccacg gtacggcatg tgattgaggt
     5161 tatggcgcgt catggacgtg atgaacagga gcagttactg gttccgggga taccggaagc
     5221 ggtgacggag gaagaagcct ttaatgccat gattcggttc tgccgtgagg tcagacgccg
     5281 ggtcagttat ccaaacagaa cgaggaccag aggatgagta aagtcgtacg cattattttc
     5341 gaatacaagg agcacgttat ccataaaaac gctgatggaa cagtgcgcat gggggtaagt
     5401 ctggacatac gttcaaccgg gataaagcag aaaggtgatg gacccgccat gatttttggg
     5461 gtggttatgc tcgcggaaag cagagacttt gctgaacttg tggcaatgaa agccagtgcg
     5521 ctcatgaaag atatgagcat gcgttccggg gttattaaag gtaatgaatt taatcagcag
     5581 gggtaattcc atgagcaaag tacgcgttat ttttgaattt aagcatgttt cgcatgacga
     5641 aaaaccggca ggcaatgact gtgttgaagt gcatgaaaag attggtgtgg atgtaaaaac
     5701 agaacgtgat acgaataaca ggccgacgtc actctgtgac gtttatgcaa gtattctcca
     5761 gtatcacagt cctgaaatta ttcagtttct ctcagcggaa tttcaggcat ctgtacaggc
     5821 ttttggggcg gatgccatca ttaaacgcca ccgcgtgcat aaagcatcag gcacactgca
     5881 ataaggaaaa acaaaatggc aaaacgcgtt acaaaattaa aggccgcagc agaggcggca
     5941 ccgcagaccc gtgaagaggt cagccgcgat atccgcaccc tgggcgatat tcagcgagag
     6001 gcgctgcggc tggaaacggc gatgaatgat gaagtggcag aaatcaccgc ccgttatacg
     6061 ccgcagattg aaaaccttaa aaaagaaatc aaagtgcttt ttaaggggat tcaggactgg
     6121 tgcaaaacca accgagatga gctgacgaac ggcggcaaaa ccaaaactgc caatctgacc
     6181 accggaacgg tgtcatggcg actggggaat ccatcatgca gcgtcagtcg tgatgtggaa
     6241 ggtgtgattg aaatgctgcg ccgtatgggc cttgagcgtt ttatccgcac gaaagaggaa
     6301 gtgaacaagg aagcggtcct ggcggagccg gatgcggtga aagggattgc cggtattaag
     6361 gtgaataaag gcgctgaaag tttttatgtc gagccttttg aacaggacgc tggactgaat
     6421 aaataacacc gcattaatca attaaatatc acttcgtttt aattatggcg ctcgcgtcag
     6481 gggactgcct gcgcctgtaa actgaaaata aggaacagga tatatggcat atttttattt
     6541 caaattagac cgtgtgcaga caaataaata tttcaccaaa tatcaacaga ctgttttacc
     6601 gctacgcaac agtattcttc gggcattact gaaaaataca ggcgctgccg gattgcgctt
     6661 aaagccgttt gccatggacg taatcagtga gttttatttt tctggtgctc tgcctgcggg
     6721 ctggcgtaag cgcgatgatg tggcttttgt cggggacgga ccgtgcttta ttgccagacc
     6781 tgatgagtca tgccctgaag gaccggcgat tgccgcaatg attgaagccg ctgaacgcga
     6841 gttaagaaag cgtcctgatt tccttgtctg gctctgtgaa aagctggggg taatgagaat
     6901 cccctccatg tttaacatgg actcctggtg gaccccgtcg ctctcccgtg atgccctgtg
     6961 cgtggtgttt aaagtaggcg cttatggcag ggaaataaaa gggggtattc ctgaagaatg
     7021 ccaggagatt aaacattctg aatatgtggc gctgacggag gaataattca tgattgatgc
     7081 aaaagtgctt gaaggggtta aaaactggct gagtatttac gggcgtctga cctgcggcat
     7141 tctggctgaa aaaatgaata tgccgccatc ctcgatggtt tattttctgc gtgatgcggt
     7201 cgatgccggg gtgctgacgg aatgtaacgg tttttatgat attccgcgtc cccgcccggt
     7261 gcagccggtt cgtcgcaaat gcagccagga aggtgcggct gatgatgttc agtggtgcag
     7321 cttcagaaaa tccctgccgt ggattgaggg gcatgatatt ccgtcaatgg cgtgggaatt
     7381 tgctcagggc gttctgacct gtgaaaccgt ttatgtggtg gctgaagttg atgagcaggc
     7441 catgaaagaa ggcgtgcccc agtttgtgat ggcgtatatc gacatacgcc tgggtgtcat
     7501 tatctgcggt ttaagcggct ggaatatcac ctcgcgcgtt ctgcgttacc tgattgttga
     7561 ccggacggcc gcgcctgccg ggatatctgc ggaggtggcg taatgttctt taaaacatca
     7621 aacccttccg cgctggccgc gtggcaaaaa tatcagcagg actgccagaa agttaaggat
     7681 gaggcaaaac gccttgaggc cgtgctgaat gttgcgtgcc ggtcggtatt tgtatccggt
     7741 atcagtggct tttgttttaa agggctgcgc tttatggatg acaaatatcc ttttcatcgc
     7801 gacttatggc gaaaaccgac tgcgtcgaat ggctggagct gcacaccgcg cacatcacgt
     7861 attcccaaag ccctgcgcgt tgcctctgac gaacttaaca gtctgtggcg tgaatattcg
     7921 cccgtcacgt atgccagaac cgatgcactg ttgttctggc tgggtattga tttctcggca
     7981 atcatgtttg gccctgtgaa gtggttctgc gttgacgatg tgatttacct tgagtgcggc
     8041 gtaaaacccg caaatcagaa aatgaccgaa attctgtctg atgagtttta tgctgctgaa
     8101 aagcgagtca gggggtgatg tatgatgatt ttacaaccca tggggcgaaa aggtcaggca
     8161 cctgctcatg tccgtgcgtg gacacctgaa gaagatgcgc tgctgattgc gctttatcca
     8221 tccaccccgg ttaaggatat tgctgtcagg gtaaaaagaa gtttctgggg tgtacataac
     8281 cggattgttt tattacgcgg tacttacccg gagttgctca aatgcaaacg tccaagattt
     8341 aaacatgatg aagataaatt tatccgaaaa aatgccagaa cgatgaccgg taagcaaata
     8401 ggagaatatc tggggcgaga ccgggattct gtccacaatc gggcgcgata tattggcgta
     8461 agcatgaaaa aatacggaga attgctgccc ttcacccgta tacctgacga tgatgttcat
     8521 cttattcgtg aattacggga tgctgaatca ccacgacgtc ttaccttccg ggaaataggc
     8581 gagaaatttg aattatccga gagtacggtg aattttattt atcaccatcg ccggactgcc
     8641 gaggacgctg tattacggga gttaacgcca tgataaagag acttgtattt ttcgcaattt
     8701 tcgttccggt gttgggggtt ggttttatat ttgcgattac aggaaacctg tccatgatgc
     8761 cagatatttg gttctttatc aggatgtcgc tcttcctgtt tattatgaat cttcttatcg
     8821 acatatatat ccgtattact ggaaaacata aatgagaaaa atcacgtcct tgtcagagtt
     8881 acaggagatg aatatgagta ttgaattaag atcgtcttat gagtatcgca aatttcttat
     8941 cgccggaggc atgaaaccgg aagatgtgga aaaaatcgtt tcttttatgg ataaggaatg
     9001 tgataagcgg gatgtgtcag aaattattat ggatgacatg attctggatt cagtcgtggc
     9061 gttaagtccg ttatggattg tgcattctct tgcggaaatt gccaaaggca ccgataaaca
     9121 ggcagctgtt gctgcccttc agacgcttaa tgaaatgcgt atatctccac gcccgacatt
     9181 aatacagatg attttgtcca gtatggagga taaagcaaat gaatagtcta cccgctggat
     9241 gggcgcgccc tctgatggct aagaagcatc atttttttaa aactggcgat aatatcagta
     9301 tatgtaggcg gtggttatat ctggctcata atcgcgagcc ggatacattt gaaagccctg
     9361 atgactgtgc cgaatgtcgc agaagactga ataaggagaa agataatgga caatagttct
     9421 ctgttgttct ggtgcttata tatcacatcc tttttcggcg catttgttat tacccggtgg
     9481 ttatgtcgaa aaatcatctg cttttttgat aaaagacatc cggttgaacg ggcggctgat
     9541 gcgctcattc agcaggccat tgtgttatat agcggtgaat ttttctgccg gataacaacc
     9601 agagatggct ggcacataat gattattcca ccaacacacc atgcccggtg ggatgaggca
     9661 gaaaaggctt tccatgtccg taaaaaggta aatacggtat gagaggaaaa ctgatatccg
     9721 ccattcatgt ggcaaagcgc gagcttgccc tggatgatga gacttataca tccgcgctgc
     9781 tggcagccac cggcaaaacc agctgccggg atatgtcacc ggatgagtta tcccgcgtgc
     9841 tggatgtttt caaaaaacgc ggttttaaag tgcgtcagaa cccggttaac cgggccttaa
     9901 aaccgggtac ggtgaccgcc aaaattcgcg ccatctggaa ggtgatgcac cggcagggct
     9961 ttatctctga tggtgcggaa accgccctta accgctgggt gaaatcgcag acggccgcgc
    10021 aaaacggcgg cgaaggtgtg gcaaactggc agtggctgga gcaacacccc gccctggcct
    10081 cagatgtgct ggagcgtctc aagcgatggc accgccgcaa aatgctggcc gcgatgggaa
    10141 tgcccgaacg cacgctgatg gggtatgacg ccgtttgcag gcagtatgaa aaatcacttc
    10201 cccgttaact ccaaatcccg ccacaacgcg ggatttttat tttaaactta ccgggaacgc
    10261 gagaaccgga ggctgatatg gcagaaactc agatgagcat gtttggtggt gacagtgagc
    10321 aactgcacgc ccttatcgac cgccttgatg acatccctga tgatgttctc aaaaagaact
    10381 ggccgcggac cctgtcagaa ctggttgaag tcaccggcgc agaacttcag cgtcagggga
    10441 ttgagccggt actggccggt aaactggcac gcaaggtggc tgcggctcag gcagcctata
    10501 tgggcggacg gggttattac ctgccggtcg gggaatctct ctttgccgag ctgcgaaaca
    10561 atgagatatt ttcgcgctgg gaccggggcg agaaaattga atcccttcgc cgccattacc
    10621 ggatgtcaga aacccagatt tataccgtca tacgcgaaca gcgccgtctg catctggcaa
    10681 gaacgcaacc gccacttttc tgatattccg caaagcctgc cgcctctcct tcaccgttac
    10741 gctgactcag agaacatcat gagacagcgt aacaatgcca aaactccccg caccactgcg
    10801 taaaaagctg attgcccttg ttctggccgg tgccgggacg ttcacgattg ccacgcatta
    10861 caccggttac tgggaaggga aagaaaactc cacgtatatc gatcccaccg gtacacccac
    10921 catctgttac ggccataccg gcccggatgt gaaaccgggt atgaccctga cagatgaaga
    10981 atgcctggaa ctactggaaa aggacatgaa atgggccttt gcggccattg atcggcgtgt
    11041 tcaggtgccg cttacccgtg gtcagacggt ggcgctggct tcgtggatct tctgggccgg
    11101 tgaaacgaac tttcgcaact ccacgcttct gcgcctgatc aatgccgggc agatgcccgc
    11161 gtcctgtaag cagtatatcc gctggattta ttcaaagggg gtgaaactcc ccggccttga
    11221 ggcccgccgt tcggcggatg aatggttatg tcgctacgac ttgccgaaag tctgaaccgc
    11281 ttctggcgac cgctcatgat agcgctgctg tgtgcggtac tgctgttacg gggtgtcctg
    11341 tggctgcggt gaataatgcc ttaccggccc tgattgccgg ggcgtgcatg gcggcgctgg
    11401 ggattatcgc tgtactggtt gccgctatat ggggaatgca ccagaagacg cagcgccttg
    11461 aagacaataa tcaggtgctt gtgcgtgagc gggatgaggc gcgtcaggtg ctggcaaatc
    11521 agcagcacac cctgcaactc atttcacaaa tcagtgaggc ggccacgaat gaaaaacaac
    11581 agaacattca gcacagcgag gggcagcaga gcgttgtccg ccggtcgctg gcggcagtgc
    11641 ctgcggcctc tgcccctgtt cctggtgatg tggctgatcg ggtgcgccgg gccgtctgtg
    11701 aaatacgtgc ctgtgaagcc ggtgccgatc cccgctgaat ggctggctga ctgcctggtc
    11761 cctcctgcgc cggagccgtt cacgtttggg gcatcggtca cttacaacct gcaactgctg
    11821 gcggtgatca agaactgcaa cgtggacaag gccagtattc gtcgtctgga ggcgcggcga
    11881 caacatgaat ttactgatat ggccggaacg cctgctgttc cggcaggaaa gacgaagtaa
    11941 ggaaaaaggt atggatgatt cagatcgtgc tcaggctgtg atggagcggg gatcagaacg
    12001 cgccctttgt aaccggctga cacgtaagcg ccgggcaatg gatgctccgg gcagacgtgt
    12061 ctgcgcggac tgtggcggag agatccccgc cgcacgtctt gttgctgtgc cggatgccat
    12121 ccgttgtgtg aactgccaga acatcatgga ggcccgtcat gtgggtcagc atcgttaaag
    12181 attatgttgt gccgatcctt tcggcgacgg caaccgctgg cgggatattc atggcgctga
    12241 tgcgcaaaac gtttgtcccc cgcgaggcct ttgaaaaact ctccgaccgc gttgagaagg
    12301 tggaaacccg cctgtcgtca ctgccgacag aagccgaggt caaccgtctg aatgtggaaa
    12361 tcgtgaccct gcggggtgaa ctgaaaacca cgaacgccac gctccgctct gtctcctatc
    12421 agaacgaact gctgctggag caggcggtaa ggaaaaaaac acaatgagtg atttcattac
    12481 tgaagatcag cgtcttgtca ttctgcggtc gctggcggat tacaacggcg aactgggtga
    12541 atccgtgctc caggactgtc tggatgatta cggtcaccgc gtgtcccgcg acaccgttca
    12601 tactcatatt gcctggcttg ccgagcaggg gctggtgcgt aagcgcacgc tcgttaatgg
    12661 ttatttcatc gctgaactga ccggccgggg tcaggatgtg gcggaaggcc gcgccaccgt
    12721 tccgggtgtg aagaaaccac gcgcaagggg gtaacgatgg ataagccaac gcgtggccgt
    12781 gtacgcaagg tggacttgct ccccgacagc atccgtaagc cactgctgga aatgctgcgt
    12841 gaaaaacgtc tgacgcaggt ccagatacgc gaggaaatca accgtctgat ccgtgaggcg
    12901 ggcctgccgg aagagcagca gctttcaccg gcggccatca gccgggaagc ctcccgtaat
    12961 gagctgattg cccgtaacct gcgtgattta cgcgagcaga caaaagccat gatggccgaa
    13021 ctgggggaca agccaaccgg tgaaaccact gcattgatcc tggagatgtc ccgcgccctg
    13081 atgtaccgcc gtctgcgtgc cgcgaccgaa tccctgaaca gtgacagcga cgtggatatg
    13141 cgtcttatta aggacatcct cctgtcagca cagcgtgcgg aaagtgccgc cgagcgcagc
    13201 attaagcgtg aaaaagaaat cagggctgca tttgctgaag agatggcaaa cgccgtcacc
    13261 gacgagctgc gcggcgtgga cgggatgagt gaacagcttg agtcccgtat taaggggatt
    13321 ctgttgggta aagtctgatg aataacgccg aagaactctc ccccttactg acgaataccg
    13381 tctccacccg caaaattgat ctggccggtg aaaaagccct gctgggcgtg gatgtgccgg
    13441 acagccttga tttgcccggt gatatgccgg tatttctgga ttatcaggcc cgctggtttg
    13501 aggatgaaag cgaggtctgc atcgcggaaa aatcccgccg caccgggctt acctgggcgg
    13561 aagccgggcg taacgtcatt accgctgcaa aaccgaagcg gcgcggtggc cgcaatgtgt
    13621 tttatgtggg gtcaaagcag gagatggcgc tggaatatat ttctgcctgt gcactgttct
    13681 cccgtgcctt taaccagctg gcagatgctg atgtgtatga gcagaccttc tgggatcggg
    13741 ataaaaaaga agaaattctg acctacatga tccgctttcc gaacagtgga ttcaaaattc
    13801 aggcactgtc ttcccgtccg tcaaacctgc gcggccttca gggggatgtg gtgattgatg
    13861 aagccgcgtt ccacgagtca ctggatgagc ttctcaaggc ggcaatggcg ctcaccatgt
    13921 ggggcgcgcg cgtgcgtatt atctccacgc ataacggcgt cgataatctg tttaatcagt
    13981 acattcagga agcccgcgag gggcgcaagg attacagcgt ccaccgcatt accctggatg
    14041 atgccattgc ggacgggctg taccgtcgta tctgttatgt caccggccgc gaatggtcac
    14101 cggaaagcga gcagaagtgg cgtgatgatc tctacaaaaa cgccccgacc cgtgaggatg
    14161 ccgacgagga atacggctgt atcccgaaaa aatccggcgg tgcctatatt cctcacgcgc
    14221 ttattgaaat ggcgatgctc cgcgacatcc cgattctgac gtttgaagcc ccggacgact
    14281 tcatcagccg tgccgcatgg ctgcgtgaat cggaggtttt aacctggtgt gaagaacatt
    14341 taaaaccgct tttagaggcg ttaaatcccc gttcccgctt cagcttcggg gaagactttg
    14401 cccgcaccgg tgacctctcc tgctttgtgc tgctggaaat caccgaatcc ctggcaaaac
    14461 gcgaggtgtt tcgcgtggag ctgcgcaacc tgccgtatgc ccagcaggag caggtgatga
    14521 tgtacatcct gacccgcgtt ccggcgctgg tcggtgcggc gttcgacgcc accggtaacg
    14581 gcggctatct ggccgaagcc gcgctgctgg cctttggccc ggacatcatc gactgcgtga
    14641 tgttatcgcc gaagtggtac ggcgagtgga tgccaaaact gaaggccgag tttgaggatc
    14701 agaatatcct cgtcgcccgc catcagacca cgcttgatga tttgcgccat gtgaaggtgg
    14761 tgaacggtat cccgcaaatt gacaaggggc gtacgaagga ccagaacgca acggctgtga
    14821 acgcccgccg ccatggcgat tttgccgtgg cgctctgtat ggcaaaccgg gcgtcataca
    14881 tggagggctt catcctggat gaatcagcct gtcaggcgct gccggagcgg tcgcgggcca
    14941 tggagggcgg ttatcgtgat gacgatgagg catatcatga atttgatcgg gggtgctggt
    15001 agtgggacgt ataattgatc ttgacggaaa acctttctcc tttgacccgg agatgcagag
    15061 cgccgcgctg gatattccgc agattgccag ccgttatatt gaacatccgg cctcgggtat
    15121 caccccgaac cgggcggcac agtgccttcg cggggctgaa cgtggcgatc tgattgccca
    15181 gtccgatctg gccgctgaca ttgaagaaaa ggatacccac ctttttgcag agctgggcaa
    15241 gcgacgcctt gccattcagg gggtgccctg gagtattgag ccgccaccga acgccagcgc
    15301 gaatgagaaa aaggacgcgg aaatgctcga cgaatatctg cattccgccg actggtttga
    15361 tgccatgctg tttgatgcca ccgatgccat cctgaaaggc tattcctgca tggagattga
    15421 gcacgggatg ctcggtaaaa tgcacatcat ccgcgccatc cgctggcgtg acagcgggca
    15481 tttctgcctt aacccggatg atttgagcga actgcggctg cgtgacggca gccatgccgg
    15541 ggtggcgttt cagccctttg gctggatagt gcatcagtca cgttcacgca ccggttacgg
    15601 cggcgcaaca gggcttgtca gaacgcttat ctggccgttc attttcaaaa actattccgt
    15661 gcgcgatctg gctgaatttc tggaggtgta cggcctgccg atgaaggtcg gtaaataccc
    15721 gtccggggca acgccggagc agaaaagcgc cctgatgcgg gcggtgatgg atatcgggcg
    15781 acgtacaggc gggatcatcc cggccgggat gtctctggag tttcaggcag ccgcgaacgg
    15841 tcaggccgat ccgtttgaaa ccatgatttc gtggggggag cgttccatct ccaaagcaat
    15901 actcggcggc acgctgacca cggaagccgg agacaaaggc gcgcgctcgc tgggtgaagt
    15961 gcataacgag gtgcgccggg aaatcaggga ttctgattta cgccagctgg ccgccacgct
    16021 gaaccgcgat ctggtgtatc cgctgtatgc cctgaacacc gcccacgcca ttgatatccg
    16081 tcgcctgccg cgtatctgtt tccagacaaa agagccgggg gatatcacca aaatcaccag
    16141 tgcggtgatg cagctcagta cggggatgga tattcctgat ccctgggtac gggaccagac
    16201 cggtattcca cagcctgccc ccggtgaagc catcttccgt gtccgtcaga gtggcaatga
    16261 acctgctcag acagacaaag agatgccacc ggaaaaacag gaaaagacag agcagacggc
    16321 gctgtcagcc cgactgccgg aagcgaaaag cagcccccgt gatgaactgg acgatatggg
    16381 ggatgcggtg cctgcccgcc ggttacagga ggccatcgac ccggtactgg agccggtcat
    16441 tgatgccatc agaacgcggg ggctggcgga ggcactggca gacctgcctg ccctttaccg
    16501 tgaaatggat gattcccgcc tgatgacgct gctcagtgat gccatgtttg ctgcggaaat
    16561 gaaggggatg ctggatggca caggggattg atttaggtta tgccgccacc cttccctcaa
    16621 aagaggcggt ggcatacttc cgcgccaaag gggcgcatat cagctggaac tggttcgaaa
    16681 cagacgcgga tgttcatgcc cgttcattca cggcggcaag agtggcacgc ctggacgtac
    16741 tgaccacact acaggcggaa gtgcagcgtg ctgttgatga ggggatttca cagaaagcat
    16801 ttatccgcac actgacgccc cgcctgcaaa agctgggatg gtggggaaaa cagattgtgg
    16861 tggacagtgc cggtaacgcg gaagaagtgc agctgggcag tccccgccgt ctggcgctga
    16921 tttacaacgt gaacacccgt gtggcttaca atgccgggcg ttacacgcag atgatgaaca
    16981 acacggacac gcatccgttc tggcagtatg tggcggtgat ggacagccgc acccgcccgg
    17041 cgcattccgc tcttaacggt ctggtattcc gctatgacga tccgttctgg aatacacact
    17101 acccacccaa tggctggaac tgtcgctgcc gtgtccggcc attgtctcag gcccgtctgg
    17161 atgcaatggg gttatccgtt tcatccggtc aggatcatct ctccacccgc aatgttgagg
    17221 ctggcgtgga taagcagacc ggggaagtca gagaaatgcc ggtgaccaca tattcagatg
    17281 gcaccagaac catgacaccg gatgtgggct ggtcatataa ccccggttcg gcggcgttcg
    17341 gcacagacca ggcgctgatc cgtaaactga tcgaggtgaa aagcccggcg ctgcgggaaa
    17401 tggtggttca ggagatgaac aacagcccgg agcggcagct ggcgttccgc atctgggcaa
    17461 aaaacatcat gaaaacccgg cgaggcggta acgatatccg cacgctgggc tttatgaccg
    17521 aaagcattgc gcaggcggtg gaaagccgga cgggaacgcc accggcccgc ctgctggcga
    17581 tgagcggtaa aaatgtgctc catgcggaca gcatgaaaca tcagaatgac ggtatcgccc
    17641 tgacaccgga ggatttcggg cgcttaccgg caatgctggc aaggccaaaa gcggtactgt
    17701 gggacaaacg gcacaacaat ctgatgtata tcgtggaaaa tcaggatggc agtgtgcaga
    17761 ttgccgttaa tgcaccgtac agcctgaagc gtcagccgga taaactggat gtgattgtta
    17821 atgcttaccg ggttatcaat atggataagc tgaaatcgga tatccggggc ggaatgcttg
    17881 aggtactgga aggagatatt gattaacggt cagtggcggg ggtcgaaccc gcatacatga
    17941 tccacccgga ggcaggcacc gactttacca gttaagcgta cactgaccgc ctgcttattt
    18001 tatattcata ttttatacgg aggcaatatg tcatccattg atgccgccgt cgttgttgac
    18061 gttacgcgtc tccagcgggt ctttgcccgg cttcagtttg tgggtggcgg aaaagacctg
    18121 gcccgcagtg tggcgtccag ccttctgtca tcgtcagaaa tggcgtttga acaggaaaaa
    18181 gagccggacg gcgaacgctg gcatgactgg tcagatcctt accgcaagtg gcgtacccgt
    18241 aagggataca tgcccggcaa aatcctgacg ctgaacggcg atctggcccg acgcctgacc
    18301 acggattatg gcgacacctg ggcgctgatt ggatcaaatg agccttatgc ggccattcat
    18361 cagtggggtg gcctgccagg tatgccgccg ggaccggcgg ccattggtgc acgtccgtat
    18421 atgggctttg atcaggtggc agagcaggag atcatggacg aaatcagaaa acgctttaaa
    18481 aaggccacag aaacgcctta attgtttaag gtatgcaaat gcattacctt acccccttca
    18541 ggcgcgtgtc gtgattttct aacctgtatt taacgggctt taaaatctgc gtgaagcgcc
    18601 tttgtatttt tcccctggaa gcacttcctg aaatcccgca aaacccgccc tgaaaattcc
    18661 cggcctatgc tgccggaatg aagacgaaaa acacgcccaa acttgcttat gccattctga
    18721 atgccatcag cctgtccgca gacggggacg gtgactggtg tcagatcatg ccagccggtc
    18781 gggtaaaggc ccgtgacggt cgcccggaaa aaccggccga gggctggctg atcaaccatg
    18841 cagccgttga gcgcatggtc tcccgtgttg tggcgctcaa tcagccggtg aaaattgact
    18901 acaaccacca gaccctgatt gagggacatc cggcaccggc tgccggtttt gtgatggcct
    18961 caccggagaa ttttcgcttc agtgaagagc gaggtttcga ggtgcgcccg aagtggaacc
    19021 ctccggccct tgaacatctg cgtaataacg aattcccctg gttttcaccg gtgattggct
    19081 atgacgagag caccggcgaa cctgtcgaac ttcggatgct ggctattacc ggtgaccccg
    19141 gtctgaccgg catgaatcct gtcgccgcac tgtcggcgga tgacctttat aacgccttaa
    19201 accctccttt aaaggatacc tccatgaatg agcaattacg ccagttgctg acggcgctcg
    19261 gtctgaccgt ggctgacggt gacgaattta cgccggagct gggcacggcg gcgctgtctg
    19321 ccctcaccgg gatcaaaacc cgtgcggatg cacacgacaa cctgaaaaca caggtcgcca
    19381 gtctgtcggc agagctggaa accgcaaaag gcacaccggc tggcggcagt attgatctga
    19441 cgaaatacgt gcccgttgaa acgtataacg ccctgcgtac cgaatacgtt gcgctgtcgg
    19501 cgcagcacgg cagcaccacg ctggagcagg tgctggacaa ggctgaatcc gaagggcgca
    19561 tcttcaaaag cgaacgcggc tatctggaac aactgggcgg gcagattggc gttgcggcac
    19621 tttctgcaca gcttgacgcc cgccagccgg ttgcggccct gacctctctt cagaccgaca
    19681 ccgtgaccgt gccggataaa aagactgcca ccgctgtgct gtcggctgaa gatatcgcgg
    19741 ccgccaaact gctgggtaaa accgaagccg agttcctgaa aatgaaagag gaaatgcaat
    19801 aatgcctacc ccgattacac cggcgatgat caccgccctg atgacgggct accgttctga
    19861 ttttcaggcc gggatgtcca tggccccgtc gcagtacaaa aaaattgcga tgaccgtacc
    19921 gtccacgtcg aaatccaaca cctacggctg gctggggcag ttcccgcagt tccgtgagtg
    19981 gatcggctcc cgcgtcatcg agaagatgaa ggcctatggc tatgccatcg tgaacaaaac
    20041 ctttgaaggc accgtcgcca ttaaccgcga tgacttcgaa gacgataacc tcggtattta
    20101 ttccccgttg ttccaggaga tggggcgcgc tgctgcggca caaccggatg aactggtctt
    20161 tgctgctctt cgtgacggga ttaatgcggc ctgctatgac ggccagaact tctttgatac
    20221 cgaacatcct gtttacccca aagtggatgg ctccggggat gcgcagatgg tcagcaatat
    20281 gtttgtggca aaaaccggct ctgtcggtgc acaagcggat tacagcggtc ctgcctggta
    20341 tctgctcgac tgttcacgcg caatcaaacc gctgatttat caggatcgcc gtaaggctga
    20401 actggttgcc cagaccaaag tcgatgaagg tcgcgcattc accgataacg aatttgtgtt
    20461 tggtgcatcc gcccgtcgca atgtgggcta cggcttctgg cagatggcct acatgatgca
    20521 gtcaccgctg acgctggatg cactgtggca cggctggtca gccatgcgcg aatttaccgc
    20581 tgacggtggc cgcaaactcg gcatcaaacc cacccatatt gttgtcccca cctcgctgga
    20641 aaaacaggcg gtgcagctgc tggagcgtga actgttcgca gacggaaacg caaccgtctc
    20701 taacgagatg aagggcaagc tggagctggt tgtcgcggat tatctgtaaa cggtgagccg
    20761 ggcttacggc ccggcccctg cggagggcaa atatgaatga acatcacact gtggcagcaa
    20821 cagatgattc aggtcttcag gtcagtggtg acaattctgt cacagtgctg gcagaagtgc
    20881 gttgcagtcg ttcggcgttt cgtcgtgcgg ggttcctgtt cacgcgtgga cgtcagcagg
    20941 ttgaggtctc cccggagcag cttgcccgac tggaggcgga gccatgcctc actgtgcgaa
    21001 tactacagac gcctgctgat gatgcgggga gcgtggcggg tgtggttcat gcagtggccg
    21061 gtacagattt agccgaagcc ggacaggata ctccccggaa aaagaccggt aacaaagcga
    21121 aacaggcgaa aaaggcccgc gcatgaatta cgccactgaa accgatatgc gggcgcgtta
    21181 ccgcgaggat ttgctcaggc cgttactggc tgtgccccgt tcggatgagc cggacacgcg
    21241 caagctgaac cgggcgctga cagatgcgtc agcccttatc gacagctatc tgtccgcccg
    21301 ttacacgttg ccgctggagg ttattccggc cgttcttgtt cagcactgtt gcgcgattgc
    21361 cttttattac ctgtgcgatc agcgagcctc cgatcaggcg cgtgaccgtt accgtgaagc
    21421 gctggcctgg ctgaaagatg tcatgaacgg caatgtgccg gtcggtgtgg atacgaacgg
    21481 tgcggcccct gaatccgggg atttaccaca ggttcagtct gatgcagcgg tattcgggcg
    21541 caaccagaag ggcttcatat gattacggaa accgaacagg catacatcgc ccgtatccgt
    21601 gagtatttcg ggaatgaact ggtgtctgtt gacacgcatc ccggcgactg gagcgacagc
    21661 gtactgcgca ccatgttgat taacgccccg gcgatctacg ttgcctggct gggtgccggt
    21721 gaaggccgta cccgtggtcg cctggtcagt cactgggtgt tctacgtcat cggcgacatg
    21781 ctcaacgggc gtgaggccag ccgtcccgga ctgtatcaga ttgtggcccg gctgattgcc
    21841 gtgcttaacg gcttcagaac agaaaaaacc tcaccgcttt actttgaaaa ggcggtcaac
    21901 ggttacaccg aaacccaggc ggacagtggt gcggtgatgt atgcgcttta tttctcctgc
    21961 gaggaaatga tcgcaccgct gaccgatacc agctcgctgg acgacttcct gcgtcattac
    22021 gaaacctttg tggagccgcc cggtacgccg ccgtttgagg cacatattga tttgccgggg
    22081 aacacgacaa cggcggtcga accgccggag gaactgtaaa tgaaaaccat ctttatcaaa
    22141 cccgcgccgg gacgcctgat tcgtgatccc gacacaatgc gcccgctggc acaggagggg
    22201 gaggaaaagc cctttacgcc gttctggtgt cggcgtcttg atgacggcga tgtcattcag
    22261 gctgaaaaag ccgctgaaga ggctccggct gtatctgcgg atgcaacgac tgcgacagaa
    22321 aaaccggtcg cccaaccggc atccgataag gagaaaccgc aatgattaat tttgacggga
    22381 tcggtaatga ccgccgtata ccgctgattg aggttgagtt taataactcc atggctgtca
    22441 ccggcactcc ggcacagcgt cagtgtgtgc tgctgtttgg tcaggcgaga atgaaagaga
    22501 atgccgtgga cggtgccggt gttctggatg ttccggtcag gatcacccgt gcgtctcagg
    22561 caacagaact ctgggggcgc ggttccatga ttgccctgat ggtggcggaa tttatcgcca
    22621 tcaatcctga tgctgaactg tatgccattg ctcaggggaa cggcaccgga caggcaacgg
    22681 ctgcggccat gaatctgacc ggaacggtca cccgtgacgg gattgtttat gcctgcgtgg
    22741 gggggcggcg ttatacactc ccggcaccaa aaggtaagaa aggcaaagaa ctgaccgacg
    22801 agctggccgc gctgatcaat gccgatcctg acgcgccctt cacggcctcc tctggtgcag
    22861 gaagtggtga taatggcgca ggtctgaaag ggtctctggg gatcaccgcc cgctttaccg
    22921 gtgaatgctc cgttcatgat gtgcgcctga actatcacga cggggaagca accccggaag
    22981 gaatacaggt cgctatagcc tatccgaaac aaaaagcggc taacccggat atcacccgaa
    23041 gcgtggcggg gatgggcgat cgccagtaca actatgtcgt catgccctat aaggatgatg
    23101 caaacctgaa aatcatcagt gatgagcttc tgaaacgctg gggaccggcc aaaatgtctg
    23161 acggcgtgct gtggctggca cataccggca cgtttggtga ggtgcaggca ttcggggcaa
    23221 aacgcaatga ttttctggcg acctgcacat ccattcccaa agcccctgaa ccggattacc
    23281 tctgggcagc ggcggtctgt gccacctgtg caccctctct gggagcagat cctgcgcgtc
    23341 cgcttcagac gctggcgctt ccgtcacgca tggccccggc tccggccgat cgtctgacgc
    23401 gtgaagagcg taacagtctg ctgtggggag ggattgccac ggtcagtgtg gctgccggtg
    23461 atgtggttca gatcgaacgt caggtgacca tgtatcgcca gaacgtgtac ggcgaaagtg
    23521 atcccagcta tctggatgtg gaaaccatct acaccctctc ctggctgcgt tattccctgc
    23581 gtacttttat cacacaacgt tttccgcgcc ataaactggc tgatgacggg acgcccgttc
    23641 gtgccggtca gaatatcgtg acgccggaga tcatgaagct tcagttaatt tcgctgggtg
    23701 aagagtgggt ggaactcggg ctggtcgaaa atctggacac ctttaaaaag aatctgcttg
    23761 tggagcgtaa tgcgtcagat cgaaaccgtc ttgacgtgtt atgcacgccg gatctggtta
    23821 accagttccg cttcctggca gcacagatcc gtttcatttt gtgaggtaag gcatgagcgg
    23881 aaaacagtat cagggcacgg ccactatccg tgtgaacggt caggaatacg agacgcttga
    23941 gggggctaca ttttctccgt caggttttga acgtgaagtg gtgaaaggcg cgaaagtcta
    24001 tggttaccgc cagaaaccac gtgaagcgac gctggactgc aaatttccgg caggcggaga
    24061 aggctcacca gccgctgatg aaatcaacaa ctggactgcg gtcactatcg agtttgtggc
    24121 ggataccggc gaagtccaca tgatgacgaa ggcctggagc agtgaaccgg cctcgcttga
    24181 cggtggcggg gagatctccg tgaagttcgc cagcgccacc agtacccgtg ttcagtgatc
    24241 aggaaaaata aaatgaccac acgtaagaaa aaaacggcag tttccgaggc agccgtgatg
    24301 gaggcaatcc gggaagcact tgagggggct gatccacgca ctgccgggct gacagagcaa
    24361 ctggcaaagg gatatgtgga tctgcttgat ggtctgccgt ttggtgagac ccgtgaatat
    24421 cgtgtcacgt tccgggaact gacagcgaaa gacagtattg acgcagaagc agaagccgag
    24481 cgcgtggtgg agacaaacaa tggcccgatg ctgatagcgt ctccgtcgtt acgcggtgtt
    24541 gcactgctgc gccgtcagat tgccgcagtg ggagacattg aaggtccgtt gtcaccccgt
    24601 cagattggac agttaagcga gcgcgatctc tcccgcctga tggcagcggt cagcctgctg
    24661 gataccgcgc ttgccggaaa gctggcagct gaccgggggc gatcaggcgc agtgtcggga
    24721 tcagattgaa gaagcggcaa ttatcctggg gatggtgaca aaaagcggtc cggaatgggc
    24781 gcttaacctc cccctgtcgc aactttaccg gcactgccga cagacagaaa aaatcatcag
    24841 aacgaagcag taagtatggc ccgaaatctc agagcatctc tgatagttga tttgctcggc
    24901 aatatctcag ccaaatcccg ccagtggtca caggagctgg gggcattctc acgctccggc
    24961 caggcggggc tgggtggcct gggaaatgct gcccgccgtg ccgggcagga aacagatgta
    25021 ctcggaagca ggatgcagcg cacgctggcc ggggtgcggg gcagtatccg ccatgtcact
    25081 tctgattttg accgtcttca gggcagtatt accggcacca tcgggcggat cagcaatctt
    25141 tacggaatgc tggccggtgg tgctgctgta tatggcttta accggggatt tattcgcccg
    25201 gcagcggaga tggaaaccta tatgattcgc ctgaactccc tttacaaagg ggatcgggcg
    25261 aaaactgatg atgtccgcag gtgggcaata cagaatgcga aggaaaccac ctgggggctg
    25321 gctggcgtca tgcaggaata tacgtccagt ctgggctttg gtatgagtga cagggaggcc
    25381 cgaaattttg tcaccatgct tcaggatcag ggtgccgtgg gcggctggtc actgtccgat
    25441 gcacaggggg catccctgca actgaaacag atgtatgcaa gaggcagtat tcaggcggcg
    25501 gatgcaaata ttcttgccac ctacggcatt aatgcctacc gcgtgctggc tgatcgtctg
    25561 ggtgtggacc agaaagtggt cagaaaactt ggtgaaaaag ggctgcttgg gccggacagt
    25621 attcgcctgt tatttcagac gctggcggaa caggccaggg gagcacaaaa ggacgcaatg
    25681 aattcctgga gtggcctgac ggccatgatg ggtgacgtct gggaccagtt tgcccgggat
    25741 gtgatggaca gtgggccatt tgaaaaactg aagggtaatc tcaggggatt tctttcatgg
    25801 gttgacagtg ccaaatctga tggcagttac cattccctgg cggaaagcac ggcatcagcc
    25861 atgaaccagg gctttgagta tgcccgtgat gcagtgacag ggttttatca ggcgatcaga
    25921 aaggtacgcg acacgcttca ggctctgcgg gatgcgggct atggtgatgc gctggatcgc
    25981 attggtcagg gggcacagac cgccgcgaaa tacctgctgt acatgtatct cgccacccgt
    26041 gccctgaaaa tggcgcgggc tgtcggaacg gggattatac gtccgggtgc tgcattgctg
    26101 ggatacggat tatcagcggc ggccttcctg acctcacctt tccggcgctc tcctccggcc
    26161 ggaacaccac caggcacatc tcagggacga gggcaacgtt ttataaactt tcttaccggg
    26221 gtaaatccgg cagccgttca gccagtactt gtaacaaact ggcccacagg aggactgtcc
    26281 ggcaccggcg cgaccgatac atcctcaggc ggacgtcagg ggcggggccg caggaaacgc
    26341 ggaccggggc gcggaaggcc ggtcacgcct cccctgccac cgtccccagt gccaccgtct
    26401 ccctct
//
LOCUS       DAZFLD010000052        26156 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.052, whole genome shotgun sequence.
ACCESSION   DAZFLD010000052 DAZFLD010000000
VERSION     DAZFLD010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26156)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26156)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26156
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.052"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..2029)
                     /locus_tag="ACHKBL_001822"
     CDS             complement(<1..2029)
                     /locus_tag="ACHKBL_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HFO5818701.1"
                     /translation="MSQLDDTILDALTHVTFPKGFAQAEPAWVVTVDGVDYPLWQTDA
                     LVVGSGAAGLRAAVELKRRQQNVLIATAGLYMGTSACSGSDKQTLFTAATAGNGDNFT
                     KLAEALASGGAMDHDTAYVEAVGSLHTLGGLQYLGLELPEDRYGAILRYQTDHDEAGR
                     ATSCGPRTSRLMVKVLLEEVQRLAIPMLTSATVIKLLHQRDENGEDRVAGAILATGHR
                     VHNPWGLAIVIAPNVVLATGGPGELYRDSVYPHKCFGSLGLALEEGLTLTNLTESQFG
                     IGTPRSTFPWNLSGTYVQVIPHIYSVDAGGNEYNFLADYYRTTQELASNIFRKGYQWP
                     FHATRVMEFGSSLLDMAVAQEQQSGRQVFMDFNRNPEAVPGDLPFSLDRLDDDVRAYL
                     ENNDALAPSPIERLQRMNPLSISLYKMHGYDLTTQPLQFAMNNQHMNGGIEVDIWGQT
                     SLPGCFAVGEVAGTHGVTRPGGAALNAGQVFAVRLARFIGCTQKRNIDGDIAQLVAPA
                     LASIREIITQAHDNGTGMPLSVVREKIQARMSDYAGFICHADKVRRATRDALLLNEFV
                     QRHGLAIKHVGEVAELFMWRHMALTSAAVLTQLTHYIDAGGGSRGARMVIDPQGKCLP
                     QTRRGAKEEWRFRSELAEDKNHKLTIQYSQGSFITEVKSLRMQPCINGIYFE"
     gene            complement(2026..3567)
                     /locus_tag="ACHKBL_001823"
     CDS             complement(2026..3567)
                     /locus_tag="ACHKBL_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA--acetoacetyl-CoA transferase"
                     /protein_id="HFO5818702.1"
                     /translation="MRKLSTAETLAAQIQDGATIAISGNGGGMVEADHIMAAIEARFL
                     QTGHPRDLTLIHSLGIGDRDSKGTNRFAHAEMLKRIIAGHFTWSPKMQELVKSNAIEA
                     YCFPGGVIQALLREIGAGRPGLFTHVGLGSFVDPRNGGGKSNECTTDDLVELIEIDGE
                     TKLRYRPFKVDYAILRGTYADPRGNVSLEEEAIDMDSYSMALAAHNSGGKVFVQVRDV
                     LEAGAIEPRKVKLPGILVDGIVEHREQPQTYLGGYDLTISGQHRRLSSNDAIELVSHP
                     VRRLIARRAARELVAGASTNFGFGIPGGIPGVALREGVPYQSLWMSVEQGVHNGMMLD
                     DALFGCARNADAIIPSLDQFEFYSGGGIDITFLGMGEMDRYGNVNVSHLNGNLIGPGG
                     FLEIAQNARKVVFCGTFDAKGSKIDVTPDGLHIAQSGQIPKLVTQVEKITFSAAYAQQ
                     SGQEVLYITERAVFQLTAEGVELIEIAPGVEIERDILPYMAFRPIIKHPRLMESSLFT
                     PMEDA"
     gene            complement(3577..4353)
                     /locus_tag="ACHKBL_001824"
     CDS             complement(3577..4353)
                     /locus_tag="ACHKBL_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001283531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HFO5818703.1"
                     /translation="MSDQPVLFSRAAASCRLTLNREDKCHAINEEMIESLDHYLNEIE
                     NDTTLRLVELTATGDKFFCAGGDIKSWSAYSPLDMGRKWIKRGNEVFNRLRNLPQLTV
                     ANLNGHTIGGGIELALCCDIRIARPGAKFSNPEVMLGMVPGWMGIERVLNQVGPVVGR
                     QMLMLGKRLTAQEAQVANLIDEVVEKEQVESWMANQLAQLEKCGPVALAHIKQLILAL
                     ENKHADYSHQLLAGLMSATQDCQQATHAFAEKAGVSFHNQ"
     gene            complement(4363..5205)
                     /locus_tag="ACHKBL_001825"
     CDS             complement(4363..5205)
                     /locus_tag="ACHKBL_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFO5818704.1"
                     /translation="MRDLLQRPDLFSINTATLGYKTPLPAIIDACAARGIGAIAPWRR
                     ELQGEDLQQIARQLAASNINVSGLCRSTYYTAPTLAERKLAIDDNRRALDDAAVLNAA
                     CYMQVVGGLPMGTKDLYEAREQVKQGIRQLLPHSKEVGVPIALEPLHPMTAADRSCLC
                     TLRQALDWCDELDPDGEFGLGVAVDVYHVWWDPDLASQILRAGKRILAFHVSDWLVPT
                     TDLVNDRGMPGDGVINIPSIRRLVENAGFNGAIELEIFSPYWWQKDINSTLDISVDRI
                     AHYC"
     gene            complement(5215..6006)
                     /locus_tag="ACHKBL_001826"
     CDS             complement(5215..6006)
                     /locus_tag="ACHKBL_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-ketoacyl-ACP reductase"
                     /protein_id="HFO5818705.1"
                     /translation="MKSTRPVAVITGAARGIGKGCALELARGGFNLLINDRPDADSVE
                     KLHATQQECLAEGVEVICFPADVGDLSLHEEMLDAAQNQWGRLDCLLNNAGISVKKRG
                     DLLDLEPDSFDQNIAINTRAPFFLAQAFSKRLLAQPKPEAELPHRSIIFVSSINAIML
                     AMNRGEYTIAKTAVSAAARLFAARLCNEQIGVYEVRPGLIKTDMTIPATAYYDELIAK
                     GLVPWGRWGYPADIASTVRAMAEGKLIYTCGQAVAIDGGLSMPRF"
     gene            6219..6866
                     /locus_tag="ACHKBL_001827"
     CDS             6219..6866
                     /locus_tag="ACHKBL_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFO5818706.1"
                     /translation="MYLDTSNVQSLKEKLLLCAVNEFAEYGYEGARVDNIVKAAGCSK
                     QTVYHHFGNKENLFIEVLEYTWNDIRQKEKALDFSDLPPQKAIEKIIDFTWDYYISNP
                     WFLKIVHSENQSKGVHYAKSQRLLEINHAHLQLMESLLDEGKKNNIFKPDIDPLQVNI
                     NIAALGGYYLINQHTLGLVYHISMVSPQALEARRKVIKETILSWLLVDPSSTARE"
     gene            complement(6850..7488)
                     /gene="ytfB"
                     /locus_tag="ACHKBL_001828"
     CDS             complement(6850..7488)
                     /gene="ytfB"
                     /locus_tag="ACHKBL_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YtfB"
                     /protein_id="HFO5818707.1"
                     /translation="MPGRFELKPTLEKVWHAPDNFRFMDPLPPMHRRGIIIAAIVLVV
                     GFLLPSDDTPNAPVVTREAQLDIQSQSQPPTEEQLRAQLVTPQNDPDQVAPVAPEPIQ
                     EGQPEEQPQTTHTQPFQPDSGIDNQWRSYRVEPGKTMAQLFRDHGLPATDVYAMAQVE
                     GADKPLSNLQNGQMVKIRQNASGVVTGLTIDTGNNQQVLFTRQPDGSFIRAR"
     gene            7706..8326
                     /gene="fklB"
                     /locus_tag="ACHKBL_001829"
     CDS             7706..8326
                     /gene="fklB"
                     /locus_tag="ACHKBL_001829"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015742582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFO5818708.1"
                     /translation="MTTPTFDTIEAQASYGIGLQVGQQLSESGLEGLLPEALVAGIAD
                     ALEGKHPAVPVDVVHRALREIHERADAVRRQRFQAMAAEGVKYLEENAKKEGVNSTES
                     GLQFRVINQGEGAIPARTDRVRVHYTGKLIDGTVFDSSVARGEPAEFPVNGVIPGWIE
                     ALTLMPVGSKWELTIPQELAYGERGAGASIPPFSTLVFEVELLEIL"
     gene            8635..10047
                     /gene="cycA"
                     /locus_tag="ACHKBL_001830"
                     /gene_synonym="dagA"
     CDS             8635..10047
                     /gene="cycA"
                     /locus_tag="ACHKBL_001830"
                     /gene_synonym="dagA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine/D-alanine/glycine transporter"
                     /protein_id="HFO5818709.1"
                     /translation="MVDQVKVVADDQAPAEQSLRRNLTNRHIQLIAIGGAIGTGLFMG
                     SGKTISLAGPSIIFVYMIIGFMLFFVMRAMGELLLSNLEYKSFSDFASDLLGPWAGYF
                     TGWTYWFCWVVTGMADVVAITAYAQFWFPDLSDWVASLAVIVLLLTLNLATVKMFGEM
                     EFWFAMIKIVAIVSLIVVGLVMVAMHFQSPTGVEASFAHLWNDGGWFPKGLSGFFAGF
                     QIAVFAFVGIELVGTTAAETKDPEKSLPRAINSIPIRIIMFYVFALIVIMSVTPWSSV
                     VPEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSGVFSTSRMLFGLAQEGVAPKA
                     FAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMITTVSAILFMFVWTIILC
                     SYLVYRKQRPHLHEKSIYKMPLGKLMCWVCMAFFVFVLVLLTLEDDTRQALLVTPLWF
                     IALGLGWLFIGKKRAAELRK"
     gene            complement(10092..10754)
                     /gene="ytfE"
                     /locus_tag="ACHKBL_001831"
     CDS             complement(10092..10754)
                     /gene="ytfE"
                     /locus_tag="ACHKBL_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HFO5818710.1"
                     /translation="MAYRDQPLGELALSIPRASALFRKYDMDYCCGGKQTLARAAARK
                     ELDVDVIEAELAKLAEQPIEKDWRSAPLAEIIDHIIVRYHDRHREQLPELILQATKVE
                     RVHADKPSVPKGLTKYLTMLHEELSSHMMKEEQILFPMIKQGMGSQAMGPISVMESEH
                     DEAGELLEVIKHTTNNVTPPPEACTTWKAMYNGINELIDDLMEHISLENNVLFPRALA
                     GE"
     gene            complement(10862..11827)
                     /gene="ytfF"
                     /locus_tag="ACHKBL_001832"
     CDS             complement(10862..11827)
                     /gene="ytfF"
                     /locus_tag="ACHKBL_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFO5818711.1"
                     /translation="MISGVLYALLAGLMWGLIFVGPLIVPEYPAMLQSMGRYLALGLI
                     ALPIAWLGRVRLRQLARRDWLTALMLTMMGNLIYYFCLASAIQRTGAPVSTMIIGTLP
                     VVIPVFANLLYSQRDGKLAWGKLAPALVCIGIGLACVNIAELNHGLPDFDWARYTSGI
                     VLALVSVVCWAWYALRNARWLRENPDKHPMMWATAQALVTLPVSLIGYLVACYWLNTQ
                     TPGFSLPFGPRPLVFISLMVAIAVLCSWVGALCWNVASQRLPTVILGPLIVFETLAGL
                     LYTFLLRQQMPPLMTLSGIALLVVGVVIAVRAKPEKPLTESVSES"
     gene            complement(11935..12795)
                     /gene="qorB"
                     /locus_tag="ACHKBL_001833"
     CDS             complement(11935..12795)
                     /gene="qorB"
                     /locus_tag="ACHKBL_001833"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HFO5818712.1"
                     /translation="MIAITGATGQLGHYVIESLMKTVPASQIVAIVRNPAKAQALTAQ
                     GITVRQADYGDEAALTSALQGVEKLLLISSSEVGQRAPQHRNVINAAKAAGVKFIAYT
                     SLLHADSSPLGLADEHIETEKMLADSGIVYTLLRNGWYTENYLASAPAALEHGVFIGA
                     AGDGKIASATRADYAVAAARVISEAGHEGKVYELAGDSAWTLTQLAAELTKQSGKQVT
                     YQNLSEADFAAALKSVGLPDGLADMLADSDVGASKGGLFDDSKTLSKLIGRPTTTLAE
                     SVSHLFNVNN"
     gene            12884..13264
                     /gene="ytfH"
                     /locus_tag="ACHKBL_001834"
     CDS             12884..13264
                     /gene="ytfH"
                     /locus_tag="ACHKBL_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFO5818713.1"
                     /translation="MSQVSLSQQLKEGNLFAEQCPSREVLKHVTSRWGVLILVALREG
                     THRFSDLRRKMGGVSEKMLAQSLQALEQDGFLNRIAYPVVPPHVEYSLTPLGEQVSEK
                     VAALADWIELNLPEVLAVRDERAA"
     gene            complement(13393..15336)
                     /gene="cpdB"
                     /locus_tag="ACHKBL_001835"
     CDS             complement(13393..15336)
                     /gene="cpdB"
                     /locus_tag="ACHKBL_001835"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008663 - 2',3'-cyclic-nucleotide
                     2'-phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HFO5818714.1"
                     /translation="MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKD
                     TATEKFGLVRTASLINDARNEVKNSVLVDNGDLIQGSPLADYMSAKGLKAGDIHPVYK
                     ALNTLDYTVGTLGNHEFNYGLDYLKNALAGAKFPYVNANVIDARTKQPMFTPYLIKDT
                     EVVDKDGKKQTLKIGYIGVVPPQIMGWDKANLSGKVTVNDITETVRKYVPEMREKGAD
                     VVVVLAHSGLSADPYKVMAENSVYYLSEIPGVNAIMFGHAHAVFPGKDFADIEGADIA
                     KGTLNGVPAVMPGMWGDHLGVVDLQLSNDSGKWQVTQAKAEARPIYDIANKKSLAAED
                     SKLVETLKADHDATRQFVSKPIGKSADNMYSYLALVQDDPTVQVVNNAQKAYVEHYIQ
                     GDPDLAKLPVLSAAAPFKVGGRKNDPASYVEVEKGQLTFRNAADLYLYPNTLIVVKAS
                     GKEVKEWLECSAGQFNQIDPNSTKPQSLINWDGFRTYNFDVIDGVNYQIDVTQPARYD
                     GECQMINANAERIKNLTFNGKPIDPNAMFLVATNNYRAYGGKFAGTGDSHIAFASPDE
                     NRSVLAAWIADESKRAGEIHPAADNNWRLAPIAGDKKLDIRFETSPSDKAAVFIKEKG
                     QYPMNKVATDDIGFAIYQVDLSK"
     gene            15526..16266
                     /gene="cysQ"
                     /locus_tag="ACHKBL_001836"
     CDS             15526..16266
                     /gene="cysQ"
                     /locus_tag="ACHKBL_001836"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HFO5818715.1"
                     /translation="MLDQVCQLARNAGDAIMQVYDGTKPMDVVSKADNSPVTAADIAA
                     HTVIMDGLRTLTPDIPVLSEEDPPGWEVRQHWQRYWLVDPLDGTKEFIKRNGEFTVNI
                     ALIDHGKPILGVVYAPVMNVMYSAAEGKAWKEECGVRKQIQVRDARPPLVVISRSHAD
                     AELKEYLQQLGEHQTTSIGSSLKFCLVAEGQAQLYPRFGPTNIWDTAAGHAVAVAAGA
                     HVHDWQGKPLDYTPRESFLNPGFRVSIY"
     gene            complement(16256..16813)
                     /gene="ytfJ"
                     /locus_tag="ACHKBL_001837"
     CDS             complement(16256..16813)
                     /gene="ytfJ"
                     /locus_tag="ACHKBL_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001498728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HFO5818716.1"
                     /translation="MTLRKILALTCLLLPMMASAHQFETGQRVPPIGITDRGELVLDK
                     DQFSYKTWNSAQLVGKVRVLQHIAGRTSAKEKNATLIEAIKSAKLPHDRYQTTTIVNT
                     DDAIPGSGMFVRSSLESNKKLYPWSQFIVDSNGVARGAWQLDEESSAVVVLDKDGRVQ
                     WAKDGALTQEEVQQVMDLLHKLINK"
     gene            17138..17344
                     /gene="ytfK"
                     /locus_tag="ACHKBL_001838"
     CDS             17138..17344
                     /gene="ytfK"
                     /locus_tag="ACHKBL_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1107 domain-containing protein"
                     /protein_id="HFO5818717.1"
                     /translation="MKIFQRYNPLQVAKYVKILFRGRLYIKDVGAFEFDKGKILIPKV
                     KDKLHLSVMSEVNRQVMRLQTEMA"
     gene            complement(17406..18749)
                     /gene="paeA"
                     /locus_tag="ACHKBL_001839"
     CDS             complement(17406..18749)
                     /gene="paeA"
                     /locus_tag="ACHKBL_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFO5818718.1"
                     /translation="MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINA
                     QRVLNMQENPGMFFTVVQIGLNAVAILGGIVGDAAFSPAFHSLFSRYMSAELSEQLSF
                     ILSFSLVTGMFILFADLTPKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANI
                     IFRIFKLPMVRKDDITSDDIYAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTP
                     RENVIWFDLHEDEQSLKNKVAEHPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLA
                     LNSGVQIRNTLIVPDTLTLSEALESFKTAGEDFAVIMNEYALVVGIITLNDVMTTLMG
                     DLVGQGLEEQIVARDENSWLIDGGTPIDDVMRVLDIDEFPQSGNYETIGGFMMFMLRK
                     IPKRTDSVKFAGYKFEVVDIDNYRIDQLLVTRIDSKATALSPKLPDAKDKEESVA"
     gene            complement(19072..19710)
                     /gene="msrA"
                     /locus_tag="ACHKBL_001840"
     CDS             complement(19072..19710)
                     /gene="msrA"
                     /locus_tag="ACHKBL_001840"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFO5818719.1"
                     /translation="MSLFDKKHLVSPADALPGRNTPMPVATLHAVNGHSMTNVPDGME
                     IAIFAMGCFWGVERLFWQLPGVYSTAAGYTGGYTPNPTYREVCSGDTGHAEAVRIVYD
                     PSVISYEQLLQVFWENHDPAQGMRQGNDHGTQYRSAIYPLTPEQDAAARASLERFQAA
                     MLAADDDRRITTEIANATPFYYAEDDHQQYLHKNPYGYCGIGGIGVCLPPEA"
     gene            19916..21649
                     /gene="tamA"
                     /locus_tag="ACHKBL_001841"
     CDS             19916..21649
                     /gene="tamA"
                     /locus_tag="ACHKBL_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HFO5818720.1"
                     /translation="MRYIRQLCCVSLLCLSGSAVAANVRLQVEGLSGQLEKNVRAQLS
                     TIESDEVTPDRRFRARVDDAIREGLKALGYYQPTIEFDLRPPPKKGRQVLIAKVTPGV
                     PVLIGGTDVVLRGGARTDKDYLKLLDTRPAIGTVLNQGDYENFKKSLTSIALRKGYFD
                     SEFTKAQLGIALGLHKAFWDIDYNSGERYRFGHVTFEGSQIRDEYLQNLVPFKEGDEY
                     ESKDLAELNRRLSATGWFNSVVVAPQFDKARETKVLPLTGVVSPRTENTIETGVGYST
                     DVGPRVKATWKKPWMNSYGHSLTTSTSISAPEQILDFSYKMPLLKNPLEQYYLVQGGF
                     KRTDLNDTESDSTTLVASRYWDLSSGWQRAINLRWSFDHFTQGEITNTTMLFYPGVMI
                     SRTRSRGGLMPTWGDSQRYSIDYSNTAWGSDVDFSVFQAQNVWIRTLYDRHRFVTRGT
                     LGWIETGDFDKVPPDLRFFAGGDRSIRGYKYKSIAPKYANGDLKGASKLITGSLEYQY
                     NVTGKWWGAVFVDSGEAVSDIRRSDFKTGTGVGVRWESPVGPIKLDFAVPVADKDEHG
                     LQFYIGLGPEL"
     gene            21646..25425
                     /gene="tamB"
                     /locus_tag="ACHKBL_001842"
     CDS             21646..25425
                     /gene="tamB"
                     /locus_tag="ACHKBL_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamB"
                     /protein_id="HFO5818721.1"
                     /translation="MSLWKKISLGVVIVILLLLGSVAFLVGTTSGLHLVFKAADRWVP
                     GLDIGKVTGGWRDLTLSDVRYEQPGVAVKAGNLHLAVGLECLWNSSVCINDLALKDIQ
                     VNIDSKKMPPSEQVEEEEDSGPLDLSMPYPITLTRVALDNVNIKIDDTTVSVMDFTSG
                     LNWQEKTLTLKPTSLKGLLIALPKVAEAAQEEVVEPKIENPQPEEKPLGETLKDLFSR
                     PVLPEMTDVHLPLNLNIEEFKGEQLRVTGDTDITVRTMLLKVSSIDGNTKLDALDIDS
                     NQGIVNASGTAQLSDNWPVDITLNSTLNVEPLKGEKVKLKVGGALREQLEIGVNLSGP
                     VDMDLRAQTRLAEAGLPLNVEVNSKQLYWPFTGEKQYQADDLKLKLTGKMTDYAISMR
                     TAVKGQEIPPATITLDAKGNEQQVNLDKLTVAALEGKTELKALLDWQQAISWRGELTL
                     NGINTAKEIPEWPSKLNGLIKTRGSLYGGTWQMEVPELKLTGNVKQNKVNVDGTLKGN
                     SYMQWMIPGLHLELGPNSAEVKGELGVKDLNLDATINAPGLDNALPGLGGTAKGLVKV
                     RGTVEAPQLLADITARGLRWQELSVAQVRVEGDIKSTDQIAGKLDVRVEQISQPDVNI
                     NLVTLNAKGSEKQHELQLRIQGEPVSGQLNLAGSFDRKEERWKGTLSNTRFQTPVGPW
                     SLTRDIALDYRNKEQKISIGPHCWLNPNAELCVPQTIDAGAEGRAVVNLNRFDLAMLK
                     PFMPETTQASGIFTGKADVAWDTTKEGLPQGSITLSGRNVQVTQTVNDAALPVAFQTL
                     NLTAELRNNRAELGWTIRLTNNGQFDGQVQVTDPQGRRNLGGNVNIRNFNLAMINPIF
                     TRGEKAAGMVSANLRLGGDVQSPQLFGQLQVTGVDIDGNFMPFDMQPSQLAVNFNGMR
                     STLAGTVRTQQGEIYLNGDADWSQIENWRARVTAKGSKVRITVPPMVRMDVSPDVVFE
                     ATPNLFTLDGRVDVPWARIVVHELPESAVGVSSDVVMLNDNLQPEEAKTASIPINSNL
                     IVHVGNNVRIDAFGLKARLTGDLNVVQDKQGLGLNGQINIPEGRFHAYGQDLIVRKGE
                     LLFSGPPDQPYLNIEAIRNPDATEDDVIAGVRVTGLADEPKAEIFSDPAMSQQAALSY
                     LLRGQGLESDQSDSAAMTSMLIGLGVAQSGQIVGKIGETFGVSNLALDTQGVGDSSQV
                     VVSGYVLPGLQVKYGVGIFDSIATLTLRYRLMPKLYLEAVSGVDQALDLLYQFEF"
     gene            25428..25769
                     /gene="ytfP"
                     /locus_tag="ACHKBL_001843"
     CDS             25428..25769
                     /gene="ytfP"
                     /locus_tag="ACHKBL_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HFO5818722.1"
                     /translation="MRIFVYGSLRHKQGNSHWMTNAQLLGDFSIDNYQLYSLGHYPGA
                     VPGNGTVHGEVYRIDNATLAELDALRTRGGEYARQLIQTPYGSAWMYVYQRPVDGLKL
                     IESGDWLDRDK"
     gene            25936..>26156
                     /locus_tag="ACHKBL_001844"
     CDS             25936..>26156
                     /locus_tag="ACHKBL_001844"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5818723.1"
                     /translation="MFDREMINENKEAFFLYVNGRGELSAQCVIKVAEKGDYLQGFSL
                     TRRAFRTFLKDRVIQMFSSDQELNKVASET"
BASE COUNT         6296 a         6633 c         6908 g         6319 t
ORIGIN      
        1 tttcaaagta aataccgtta atacacggtt gcatacgtaa agactttact tcggtaataa
       61 aagaaccttg cgaatattga attgttaatt tgtgattttt gtcttcagcg agttcagagc
      121 gaaaacgcca ttcttctttt gcgccgcgac gagtttgtgg taggcatttg ccttgtggat
      181 caataaccat acgcgcccca cggctaccac caccagcatc aatataatgc gttaattgtg
      241 ttaataccgc ggcagaagtc agcgccatat gccgccacat aaacagctcg gcaacttcac
      301 ccacatgttt gattgccaat ccatgccgtt ggacaaattc gttcaatagc agggcatcac
      361 gagtggcacg tcggacttta tcggcatggc aaataaatcc cgcatagtca gacattcgtg
      421 cctgaatttt ttctctcaca accgacaacg gcatcccggt cccgttatcg tgcgcctgag
      481 taatgatctc tcttatagaa gccagtgccg gagctaccag ctgtgctata tctccatcaa
      541 tattacgttt ttgcgtgcaa ccaataaaac gtgccagacg aacagcaaaa acctgcccgg
      601 cattcaatgc cgcaccacca gggcgagtga cgccgtgtgt gccagcaact tcccccacgg
      661 cgaaacagcc gggcagggag gtttgtcccc agatatccac ttcaatgccg ccattcatat
      721 gctgattatt catggcaaac tgcaaaggct gtgtggtgag atcgtaaccg tgcatcttat
      781 acagcgagat agacagcgga ttcattcgtt gcagccgttc gatgggcgat ggtgccagag
      841 cgtcgttatt ttccagatac gcgcgaacgt cgtcgtccag tcgatctaat gagaagggca
      901 ggtcacccgg caccgcttca ggattgcgat tgaaatccat aaatacctga cgccctgatt
      961 gctgctcttg cgctacagcc atatctaaca agctggagcc aaactccatt acccgagtgg
     1021 cgtggaacgg ccactgatag cctttacgaa aaatgtttga agccagctcc tgggtggtgc
     1081 gatagtaatc cgcgaggaag ttatactcgt tgcccccggc atccacggaa tagatatgtg
     1141 ggatcacctg cacataggtg ccggacaaat tccacggaaa cgtgctgcgc ggtgtgccta
     1201 taccaaactg gctttcagtc aaattggtta gcgtcagacc ttcttccagc gccagcccca
     1261 gcgagccgaa acatttgtgt gggtacacac tgtcgcgata aagctcgcca ggccctcctg
     1321 ttgccagtac cacattgggc gcaatcacaa ttgccagccc ccatgggtta tggacgcgat
     1381 gaccggtcgc gaggatagcc cccgccacgc ggtcttcgcc gttttcgtca cgctgatgca
     1441 gcagtttaat cactgttgca ctggtcaaca ttgggatagc aagacgctgt acttcttcca
     1501 gcagcacttt caccattaac cttgaggtac gtggcccaca cgaggttgca cgcccggctt
     1561 cgtcatggtc ggtttgataa cgaagaatcg caccatagcg atcttccggt aattccagac
     1621 caagatattg caacccgcca agagtgtgca gagaacctac cgcttcgaca taagcagtgt
     1681 cgtgatccat cgccccacca ctcgccagtg cctctgccag tttggtgaag ttgtcgccgt
     1741 tgcccgcggt agcggcggta aacagcgtct gtttatcgga accggagcag gccgatgttc
     1801 ccatgtataa cccggcggtg gcgattaaca cattttgctg gcggcgttta agttccacag
     1861 ctgcacgcag cccggctgca ccactgccga ctaccagggc atctgtttgc caaagtgggt
     1921 aatcaacacc gtctaccgtg acaacccatg cgggttctgc ctgtgcaaaa ccctttggga
     1981 aagtaacgtg cgtcagcgca tcaaggatgg tgtcatcaag ctgactcatg catcctccat
     2041 cggcgtaaac aggctacttt ccatcaggcg tgggtgcttg ataattggac ggaaagccat
     2101 atacggcaga atgtcgcgct caatctccac acccggcgcg atttcaatta attcaacgcc
     2161 ttctgccgtt aactggaata ctgcgcgttc agtgatatac agcacttcct gaccactttg
     2221 ctgtgcgtag gcggcgctaa aagtgatttt ttccacttgg gtaaccagtt tagggatttg
     2281 acctgactgg gcgatatgca ggccatcagg cgttacatca atcttgctgc ctttggcgtc
     2341 aaacgtaccg cagaacacca ctttacgggc gttttgcgca atttcgagga agccgccggg
     2401 gccaatgaga ttgccattga gatgggatac gttgacgtta ccgtaccgat ccatctctcc
     2461 catgccgagg aaggtgatat cgataccgcc gccactgtag aattcgaatt gatcgagtga
     2521 tggaataatg gcgtcggcgt tacgggcgca gccgaacaga gcatcatcca gcatcatgcc
     2581 gttatgtacg ccttgctcga cgctcatcca taaactttga taaggcacgc cttcgcgcag
     2641 cgcaacccca ggaataccgc ccggaatacc aaagccaaag ttggttgaag cgcctgccac
     2701 cagttcccgt gctgcccgac gggcaatcag gcgacgcacc ggatgactaa ccagttcaat
     2761 agcgtcgtta gaacttagac ggcgatgttg accgctgatc gtcaggtcgt aaccgccaag
     2821 ataggtttgc ggttgttcgc ggtgttcaac gataccatcg accagaatcc ccggtagctt
     2881 gaccttgcga ggttcaatgg cgccagcttc cagtacatcg cgcacctgta caaacacttt
     2941 gccgccactg ttgtgtgctg ccagcgccat cgaatagcta tccatatcaa tcgcttcttc
     3001 ttcgaggcta acgttgccac gcggatcggc ataggtgccg cgcaaaatcg cgtagtccac
     3061 tttgaaaggg cgatagcgga gtttggtttc accgtcgatt tcaatcagtt ctaccaggtc
     3121 gtcggtcgtg cattcattcg acttaccgcc accattgcgt ggatcaacaa acgatcccag
     3181 cccaacgtgg gtaaaaagcc ccggacgtcc ggcaccgatc tcccgcagca gtgcctgaat
     3241 tacgccacca gggaaacagt aggcttcaat agcattgctt ttcaccagtt cctgcatctt
     3301 gggcgaccag gtaaaatgtc cggcaataat gcgtttgagc atttcggcgt gagcaaaacg
     3361 gttagtgcct ttgctgtcgc gatcgccaat acccagcgag tggattaatg tcagatcgcg
     3421 cgggtgtccg gtttgcagga aacgcgcttc aatggcggcc ataatatggt cagcttccac
     3481 cataccgccg ccgttaccgc taatggcaat ggttgcgccg tcctgaattt gcgccgccag
     3541 tgtctcggcg gttgataact tacgcataca atttccttat tgattgtgga agctcacgcc
     3601 ggctttttcg gcaaatgcgt gcgtcgcctg ctggcaatcc tgagtggcgg acattaatcc
     3661 agccagtaac tggtgggagt aatcggcgtg tttattttcc agcgccagaa tcagttgctt
     3721 aatatgtgcc agtgccaccg gaccacattt ttccagctgc gctaactggt tcgccatcca
     3781 gctttccacc tgttcttttt ctaccacttc atcaattaaa ttcgccacct gggcttcctg
     3841 cgccgtcagg cgtttaccca gcatcagcat ctggcgaccg accaccgggc caacctgatt
     3901 aagcacgcgt tcaatgccca tccagccagg caccatgccg agcatcactt ccgggttaga
     3961 gaatttcgcg ccggggcgag cgatacgaat atcgcagcac aaggcaagtt caataccgcc
     4021 accgatggta tgaccgttga gattcgcaac ggttaattgg ggtaagttgc gcaggcgatt
     4081 aaaaacctcg ttaccgcgtt taatccattt tctgcccata tctaatggcg aataggccga
     4141 ccacgattta atatcgccgc ccgcacaaaa gaatttatcg ccggttgccg ttaattcgac
     4201 taatcgtaat gtggtgtcgt tttcaatttc atttaaataa tggtcgagag attcgatcat
     4261 ttcttcatta atagcatgac atttatcttc acggttcaat gtcaggcggc aagaagccgc
     4321 cgctctgctg aacagaacgg gttggtcact catgattatt ccttagcaat aatgtgcgat
     4381 gcgatcgacg ctaatatcca gcgtgctgtt aatatctttc tgccaccagt acggtgagaa
     4441 tatttccagt tcaatagcgc cattaaatcc ggcgttttca accagccgac gaattgacgg
     4501 aatattgatg acaccatctc ccggcattcc acggtcattg accagatcgg tggtcggcac
     4561 taaccagtcg gaaacatgaa acgcgaggat gcgttttccg gcacgcagga tctgactggc
     4621 gagatccggg tcccaccaga catggtagac atctaccgcc acgccgaggc caaattcgcc
     4681 atcgggatcg agttcatcac accagtccag cgcctgacgt aacgtgcaca gacaagaacg
     4741 atcggcagca gtcatcgggt gcagtggctc cagtgcaatt ggcacgccga cctctttcga
     4801 atgtggcagc aactggcgaa tcccttgttt cacctgttcc cgtgcttcgt acagatcttt
     4861 tgttcccatt ggcagaccgc caaccacctg catgtaacag gcggcattta gcacagcggc
     4921 atcgtccagc gcccggcggt tatcatcaat cgccagcttg cgctccgcca gcgtcggtgc
     4981 ggtgtagtag gtgctacggc acaaaccgga aacgtttatg ttactggcag caagctggcg
     5041 ggcgatttgc tggagatctt caccctgcaa ctccctgcgc cagggggcaa tggcgccaat
     5101 accgcgtgcg gcgcaggcat caataatggc gggtagcggc gttttatagc ccagtgtcgc
     5161 ggtattgata ctgaataaat ccgggcgttg taacagatct ctcatgattc agcctcagaa
     5221 gcgcggcata cttaagccgc cgtcgatggc gaccgcctga ccacaggtgt aaatcagttt
     5281 accttccgcc atcgcgcgga cagtggaagc aatatcagcc ggataacccc aacgtcccca
     5341 cggcaccaga cctttggcaa tcagttcgtc gtaataggcc gttgccggaa tagtcatatc
     5401 ggttttgatg agaccaggac gcacttcata tacgccaatt tgttcattac aaaggcgagc
     5461 agcaaataga cgagcagcgg cagagacggc agttttggca atggtatatt cgccgcgatt
     5521 catcgccagc atgatggcgt tgatggaact gacgaaaata atcgagcggt gcggcagttc
     5581 tgcttccggt ttcggctgtg ccagtaagcg cttactgaat gcctgagcga ggaagaacgg
     5641 tgcgcgggtg ttaatggcaa tattttggtc gaagctgtct ggttcgaggt cgagaagatc
     5701 accgcgtttt ttcactgaaa tgcctgcgtt gttgagcagg cagtccagac gtccccactg
     5761 gttttgcgct gcgtcgagca tctcttcatg cagggagaga tcgcccacat cagcagggaa
     5821 gcaaatcact tccacgcctt ccgccagaca ttcttgttgt gtggcatgta atttttctac
     5881 gctatccgca tccggacgat cattaatcag cagattaaat ccgccacgcg caagctccag
     5941 cgcacagccc tttccaatac cacgagcggc tccggtaatc acggcaacag gacgagtgga
     6001 tttcatacaa tagctctctt tttttgaatt ttgtttcctt agtgatgatt agcttgcgac
     6061 tggagggtaa aaagatcaac catctagttg gttgatatgc gatggcgatc acaggacatt
     6121 tgataacctg atgaaagtac aagtttttgc ccttttttct gaagctgaaa tcaaatcagg
     6181 ttatgatagt caccacgaag aggtcgtggg gaagcgttat gtatcttgat accagcaacg
     6241 tacagagcct gaaagaaaag ctgctgttat gcgcggttaa tgaatttgcc gaatatggtt
     6301 acgaaggggc cagagtcgat aatatcgtca aggccgcggg gtgcagtaaa cagacggttt
     6361 atcaccactt tggtaataaa gaaaatttat ttatcgaagt actggaatat acctggaacg
     6421 atattcgaca aaaagaaaag gcccttgatt tctcagatct tccgccacag aaagccattg
     6481 agaaaataat cgattttaca tgggattatt atatttctaa tccctggttt ttaaaaattg
     6541 tgcatagtga aaatcaaagt aagggtgtgc attatgcaaa gtcacagcgt cttttagaaa
     6601 ttaaccatgc gcacttgcag ttaatggaat ccttattgga tgaagggaag aaaaacaata
     6661 tttttaagcc agatatcgac ccattgcagg tgaatattaa tattgctgcg cttgggggat
     6721 attatttgat caaccagcat acgcttggtc tggtttatca catcagtatg gtttcacccc
     6781 aggcactgga agccagacgt aaggtcatca aagagacaat ccttagctgg cttttggttg
     6841 acccttcatc taccgcgcgc gaataaaact gccatccggc tggcgggtaa acaggacctg
     6901 ttgattattc cccgtatcaa tggttaagcc cgtcaccacg ccgctggcgt tctggcggat
     6961 tttcaccatc tgcccgtttt gcaaattact cagtggctta tccgcgcctt ctacctgcgc
     7021 catggcatag acatcggtag cgggcagtcc gtggtcgcgg aacagctgcg ccatcgtttt
     7081 accgggttcg acacgatagg agcgccattg attatcaatt ccgctatctg gctggaatgg
     7141 ttgcgtatgg gtggtttgcg gctgctcttc tggctggcct tcctgtattg gttctggagc
     7201 gaccggagcc acctgatcag gatcattttg tggcgtgact aattgcgccc gcagctgttc
     7261 ttctgtcggt ggttgtgatt gcgactgaat atctaactgc gcttcacgcg tcaccaccgg
     7321 cgcgttgggc gtatcatcag atggaagcag aaatccgact accaatacaa tggcggcaat
     7381 aatgatcccc cgacgatgca tcggcggcag cgggtccatg aagcgaaaat tgtccggcgc
     7441 gtgccagact ttctccaggg ttggttttag ttcaaagcgc ccgggcatgg tttcctcctg
     7501 ctccgcgtct tgttcctcaa tcatagccta tgaataagct aacgctatga tgtccgtggt
     7561 aaacccgcct ttattatatt catacgggat attgctattg tttctttttc cccgggattt
     7621 gtcatcattc ccgcgacaaa gttttaccca aagaagtgtg gctgatatgc tgcccgctac
     7681 tttataccct aagaaaggaa atacgatgac caccccaact tttgacacca tcgaagcgca
     7741 agcaagctac ggcattggtt tgcaggtagg gcaacaactg agtgaatctg gcctggaagg
     7801 gctgctgcca gaagcactgg ttgcaggcat tgccgatgcg ctggaaggca aacatccggc
     7861 tgttccggtt gatgtggtgc accgcgcgct gcgtgaaatc cacgaacgcg ccgatgccgt
     7921 tcgtcgtcag cgtttccagg cgatggctgc tgaaggtgtg aaatacctgg aagaaaacgc
     7981 caaaaaagaa ggtgtaaata gcaccgaatc tggcctgcaa ttccgcgtga tcaaccaggg
     8041 cgaaggcgca attccggcac gtaccgaccg cgttcgtgtt cattacaccg gaaaactgat
     8101 cgacggcacc gtgtttgaca gctccgttgc tcgcggcgag ccagctgaat tcccggttaa
     8161 cggcgtgatc cctggctgga ttgaagcact gactctgatg ccggtaggtt ctaaatggga
     8221 actgactatc ccgcaggaac tggcatatgg cgagcgtggc gcaggcgcgt ccatccctcc
     8281 gttcagcacc ctggtgtttg aagtcgaact gctggaaatc ctctaagcag cgcattctgt
     8341 tcccctcgaa ccagagggga gcagacattc agcaataaac ccttcagttt gccaaacggc
     8401 gctattttgt gttgcaaaga ccccgtaagc gtgtattttt gtgagctgtt tcgcgttatc
     8461 accgtgatat gacactcact ttaaacataa aattaacatt agatctaaat cttagtattc
     8521 ataccgcgta ttgttaccta atatcgatga gtcccgatac agattcgtcg tatcatagac
     8581 tgactaaagg ccgtagagcc tgaacaacac agacaggtac aggaagaaaa aaacatggta
     8641 gatcaggtaa aagtcgttgc cgatgatcag gctccggctg aacagtcgct acggcgcaat
     8701 ctcacaaacc gacatattca gcttattgcc attggcggtg ccattggtac agggttgttt
     8761 atggggtccg gcaaaacgat tagccttgcc gggccgtcga tcattttcgt ttatatgatc
     8821 atcggtttta tgctcttttt cgtgatgcgg gcaatggggg aattgctgct ttcgaatctg
     8881 gaatacaaat cttttagtga cttcgcttcc gatttactcg ggccgtgggc aggatatttc
     8941 accggctgga cctactggtt ctgctgggtt gtaaccggta tggcagacgt ggtggcgatc
     9001 acggcttatg ctcagttctg gttccccgat ctctccgact gggtcgcctc gctggcggtg
     9061 atagtgctgc tgctgacgct taacctcgcc accgtgaaaa tgttcggtga gatggagttc
     9121 tggtttgcga tgatcaaaat cgtcgccatt gtgtcgctga tagtcgtcgg cctggtcatg
     9181 gtggcgatgc actttcagtc accgaccggt gtggaagcgt cattcgcgca tttgtggaat
     9241 gatggcggct ggttcccgaa aggtttaagt ggcttctttg ccggattcca gatagcggtt
     9301 ttcgctttcg tggggattga gctggtaggt acaacagctg cggaaaccaa agatccagag
     9361 aaatcactgc cacgcgcgat taactccatt ccgatccgta tcattatgtt ctacgtcttc
     9421 gcgctgattg tgattatgtc cgtgacgccg tggagttcgg tagtcccgga gaaaagcccg
     9481 ttcgttgaac tgtttgtgtt ggtaggtttg cctgcggctg ccagcgtgat caactttgtg
     9541 gtgctgacct ctgcggcgtc ttccgctaac agcggtgtct tctctaccag ccgtatgctg
     9601 tttggtctgg cccaggaagg tgtggcaccg aaagcgttcg ctaaactctc taagcgcgca
     9661 gtacccgcga aagggctgac cttctcttgt atctgtctgc tcggcggcgt ggtgatgttg
     9721 tatgtgaatc ccagcgtgat tggcgcgttc acgatgatta caaccgtttc cgcgattctg
     9781 tttatgtttg tctggacgat tatcctttgc tcgtacctgg tgtatcgcaa acagcgtcct
     9841 catctgcatg agaagtcgat ctacaagatg ccactcggca agctgatgtg ctgggtatgt
     9901 atggcgttct ttgtgtttgt tctggtgttg ttgacactgg aagatgacac ccgccaggcg
     9961 ctgctggtta ccccgctgtg gtttatcgcg ctggggctgg gctggctgtt tattggtaag
    10021 aagcgggctg ctgaactgcg gaaataatcg cattatcatg ctggatggcg caatgccatc
    10081 cagcttttag atcactcacc cgccagcgcg cgcgggaata gtacattgtt ttccagactg
    10141 atgtgctcca tcaggtcatc aatcagttca ttaatgccgt tatacatcgc tttccaggtg
    10201 gtgcaggctt ccggcggcgg tgtgacgtta ttggtggtgt gtttgatcac ttccagcagt
    10261 tcgcccgctt catcgtgctc gctttccatt acgctgattg gccccattgc ctggctgccc
    10321 atgccttgtt taatcatcgg gaagagaatt tgctcttctt tcatcatgtg gctggaaagc
    10381 tcttcatgca gcatagtcag gtattttgtc agcccttttg gcacacttgg tttgtcggcg
    10441 tgaacgcgct cgactttagt cgcttgcagg atcagctccg gcagttgctc gcggtggcga
    10501 tcgtggtagc gcacgatgat atggtcgatg atttctgcca gcggggcgct acgccagtct
    10561 ttctcaatcg gttgttcagc aagctttgcc agttcggctt caatgacgtc aacatccagt
    10621 tctttacgtg ccgccgcgcg cgccagcgtc tgcttaccgc cacagcagta atccatatca
    10681 tatttacgaa acagagccga agcgcgagga atagagagcg ccagttcacc taaaggttgg
    10741 tcgcgataag ccatagctga tacctcattc ttaataagat aagatgtatt ttaaatgcat
    10801 ctttatggca aatgctataa cccttacgta gcagaggctt atttaactca ctgccagcgt
    10861 gtcaactttc tgagacagat tcagttaaag gtttttctgg ttttgctctg actgcaatga
    10921 ccacgccaac cactaacagc gcgataccgc ttagcgtcat tagcggcggc atttgctggc
    10981 gtaacaaaaa ggtgtacagc aagcctgcca gcgtttcgaa aacaatcagc ggcccgagaa
    11041 tcactgtcgg taatcgctgg ctggcgacgt tccagcagag tgcgccaacc catgagcaaa
    11101 gcacggctat cgcaaccatc agactaataa acaccagcgg acgggggcca aagggtaggg
    11161 agaagcccgg cgtttgcgta ttcagccagt aacaggcgac gagatagccg atgagagaaa
    11221 ccggcagcgt gaccagcgcc tgcgccgtcg cccacatcat cggatgtttg tcgggattct
    11281 cccgcagcca gcgggcgttg cgcagggcat accatgccca gcagaccacg gaaactaacg
    11341 ccagcacgat gccagaggta taacgtgccc agtcaaaatc ggggagtccg tggtttaact
    11401 cagcaatatt cacacacgcc aggccgatgc caatacaaac cagtgccggg gcgagttttc
    11461 cccacgcgag tttgccgtcg cgctggctat aaagcagatt ggcaaaaacg ggaatcacca
    11521 ccggcagggt gccaataatc atcgtggaaa caggcgcgcc agtacgttga atggcactgg
    11581 caaggcagaa gtaatagatg aggttgccca tcatggtgag catcaaggcg gtaagccagt
    11641 cgcgacgcgc caactgacgc agacgcacgc gtcccagcca ggcaatgggc agcgcaatta
    11701 accctaacgc cagataacgc cccatcgact gcaacatcgc cgggtattcc ggcacgatca
    11761 acggcccgac aaaaataagc ccccacatca accctgctaa cagggcgtac agcacgccgc
    11821 taatcattac tggcatccat tggtctgtca gaagaaagtt caggaagcag acatgattgc
    11881 tcccggcatc ccgtcattat cagagaggat gcgggaggat gccactttaa ttaactagtt
    11941 attaacatta aaaagatggc ttacgctttc ggctaacgtt gtcgttgggc ggccaatcaa
    12001 tttgctaagc gttttgctgt catcaaacag accgcctttc gatgcgccaa cgtcagaatc
    12061 cgccagcata tccgccagtc catcaggcag tccgacgctt ttcagtgccg cagcgaaatc
    12121 agcttcgctc aggttttgat aggtaacctg tttgccgctc tgtttggtga gttctgccgc
    12181 taactgtgtc agcgtccagg cactatcgcc cgccagttcg taaaccttgc cttcgtgacc
    12241 ggcttcgcta atcacgcgtg ccgcagctac cgcataatca gcccgcgttg ctgaggcaat
    12301 tttgccatcg cctgcggcac cgataaatac gccgtgctcc agtgctgccg gggcgctggc
    12361 gaggtagttt tcggtgtacc agccgttgcg cagcagggtg taaacgatgc cagaatcagc
    12421 cagcattttc tccgtctcga tgtgctcatc ggcgaggccg agcggggagg aatcggcatg
    12481 tagcaggctg gtataagcga taaatttcac gccggccgcc tttgcggcat taataacatt
    12541 acgatgctgc ggggcacgtt gacccacttc gctggaagag atcagcagta gtttttccac
    12601 tccctgaagt gcagatgtca gtgcggcttc atcgccgtaa tcagcctgac gcacggtaat
    12661 gccttgtgct gtcagtgcct gtgcttttgc cggattacga acgatagcca ctatttggct
    12721 ggcaggaacc gttttcatca aggattcaat aacatagtga ccaagttggc cagtggcacc
    12781 agtaatagcg atcatgggaa gtctccatcg ttttgtcttg tgtttaaata cgctaacact
    12841 taaacttact tttagtaagt acgtacaaaa aggtaagtat gagatgagtc aggttagcct
    12901 gtcgcaacaa ctgaaagagg gaaatctctt cgctgaacag tgtccgtcgc gcgaggtttt
    12961 gaaacacgtc accagccgtt ggggggtgtt gattctggtg gcgctacgcg aaggtactca
    13021 tcgctttagc gacctgcggc gcaaaatggg cggggtgagt gaaaagatgc ttgcgcagtc
    13081 gttacaggca ttagaacagg atggttttct taaccgtatc gcgtatccgg tggtgccgcc
    13141 gcatgtggaa tatagcctca cgccgctggg cgagcaggtg agcgaaaagg ttgctgcact
    13201 ggcggactgg attgagttga atttgcccga ggtgttagcg gtgcgggatg aacgtgcggc
    13261 ataactgccg gatgcgctgc gcttatccgg actacggttc atggcttgta gggcggataa
    13321 gatgcgtcat catcgcatcc gccaataaat gccggatgcg gttgatgatt tgtaggccga
    13381 aaaagaagtg ttttacttac tcagatccac ctgataaatc gcaaacccga tatcatcggt
    13441 cgcgacttta ttcatcggat actgcccttt ctctttaata aacactgcgg ctttatctga
    13501 cggagaggtt tcgaaacgga tatccagttt cttatcgcca gctattggtg ctaaacgcca
    13561 gttgttatct gccgccgggt gaatttcccc cgcacgtttc gactcatcag caatccacgc
    13621 tgccagcacc gagcggttct catccggtga agcaaaagcg atatggctgt cgcccgtacc
    13681 ggcaaatttg ccgccgtaag cgcgatagtt attggtggca acgaggaaca tggcgttcgg
    13741 atcaatcggc ttgccgttaa aggtcaggtt cttaatcctt tccgcattgg cattaatcat
    13801 ctggcactcg ccgtcataac gggcgggctg ggtaacatca atctgataat tcacaccatc
    13861 aataacatca aagttatagg tgcggaaacc atcccagttg atgagtgact gtggtttcgt
    13921 gctgttagga tcaatctggt taaactgtcc ggcggagcac tccagccact ctttcacctc
    13981 tttaccgctg gctttcacca caatcagcgt attggggtag agataaagat cggcggcatt
    14041 acggaaggtc aactggcctt tttccacctc cacatagctt gccgggtcat ttttgcgacc
    14101 accaacttta aacggtgcgg cagctgaaag cactggcagt tttgccagat ccggatcgcc
    14161 ctgaatgtaa tgctcgacat acgctttttg cgcgttgttc accacctgca cggtcggatc
    14221 gtcctgcaca agcgccagat agctatacat attgtcggca gatttaccga ttggcttgct
    14281 gacgaactgg cgtgtggcat cgtgatcggc tttgagtgtt tctaccagct tgctgtcttc
    14341 cgccgcgagg gattttttat tcgcgatgtc gtaaatcggc cgtgcttccg ctttcgcctg
    14401 cgtcacctgc catttaccgc tgtcattact gagttgtaag tcgaccacac caagatgatc
    14461 gccccacatg cctggcatta ccgccggaac accattcagc gtgcctttcg cgatatcagc
    14521 cccttcgata tcagcaaaat ctttacctgg gaaaacggcg tgagcatggc caaacataat
    14581 ggcgttaacg cccggaattt cactgaggta ataaactgag ttttccgcca tcactttata
    14641 cggatcggca gacagcccgg aatgcgccag aacgacaacg acatcggcac ctttctcgcg
    14701 catttcaggc acgtatttgc gcacggtttc ggtaatatca ttcaccgtca ctttcccgga
    14761 taaattagct ttatcccagc ccatgatttg tggcgggacg acgccaatat aaccaatctt
    14821 cagcgtctgt ttttttccgt ctttatcgac cacttcggtg tctttaatta aatacggtgt
    14881 aaacattggc tgtttggttc tggcgtcaat gacgttggca tttacataag ggaatttcgc
    14941 tcccgccagc gcatttttca gataatccag accgtagtta aactcgtggt tgccaagcgt
    15001 gccgacggta tagtccagcg tatttaatgc cttatagacc gggtgaatat cacctgcttt
    15061 taaacctttc gccgacatgt aatctgcaag cggacttccc tgaatcaaat cgccgttatc
    15121 gaccagtacg ctgtttttca cttcattgcg ggcatcgtta atcaggcttg cggtacgtac
    15181 cagtccgaat ttttccgtgg cggtgtcttt gtaataatcg aaatccatca tgttgctatg
    15241 cagatcagtg gtttccatga tacgcagatc gaccgtcgct gcattcacac tggcggcaat
    15301 cagcgtggcc aggagcgttg cgctaaactt aatcatcagg gacatccttt tatcatcggg
    15361 aatacgaaag aaaagggaga ataaacgtct tacttataga acagtgaaga atgccacaat
    15421 tttacgcttt gaaaatgatg acactatcac agttggcgca ttcattaacg atagggtata
    15481 agtaaaacaa taagttaaca ccgctcacag agacgaggtg gagaaatgtt agatcaagta
    15541 tgccagcttg cacggaatgc aggcgatgcc attatgcagg tctacgacgg gacgaaaccg
    15601 atggacgtcg tcagcaaagc ggacaattct ccggtaacgg cagcggatat tgccgcacac
    15661 accgttatca tggacggttt acgtacgctg acaccggata ttccggtgct ttctgaagaa
    15721 gatcctcccg gttgggaagt ccgccagcac tggcagcgtt actggctggt agacccactg
    15781 gatggtacta aagagtttat taaacgtaat ggcgaattca ccgttaacat tgcgctcatc
    15841 gaccacggca aaccgatttt aggtgtggtg tatgcgccgg taatgaacgt aatgtacagc
    15901 gcggcagaag gcaaagcgtg gaaagaagag tgcggcgtgc gcaagcagat tcaggtccgt
    15961 gatgcgcgcc cgccactggt cgttattagc cgatcccatg cagatgcaga gctgaaagag
    16021 tatctgcaac agcttggcga acatcagacc acgtccatcg gctcttcgct gaaattctgc
    16081 ctggtggcgg aagggcaggc gcagctgtac ccgcgcttcg gaccaacgaa tatttgggac
    16141 accgccgctg gtcatgccgt ggctgtagct gccggagcgc acgttcacga ctggcagggt
    16201 aaaccgctgg attacactcc gcgcgagtcg ttcctgaatc cggggttcag agtatctatt
    16261 tattaattaa tttatgcagc aggtccatca cttgctgcac ctcttcctga gtaagcgccc
    16321 cgtctttggc ccattgcacg cgaccgtctt tatccagcac cactacagcg gaactttctt
    16381 catccagctg ccaggcaccg cgcgcgacgc cattgctatc gacaataaac tgcgaccagg
    16441 gataaagctt tttattactc tccagactgc tgcgcacaaa catgccggaa cccggaattg
    16501 cgtcgtcggt gttaacaatg gtggtggtct ggtaacgatc gtgcggtaac tttgctgatt
    16561 taatcgcttc aatcagcgtc gcgtttttct cttttgcaga ggtgcgacca gcaatatgtt
    16621 gcagtactcg cacttttccc actagctgcg cgctgttcca ggttttgtag ctaaactgat
    16681 ctttatcaag caccaactcg ccacgatcgg taatgccaat cggcggcact cgctgaccgg
    16741 tttcgaactg atgtgcggaa gccatcatcg gcaacagcag gcaggtgagt gccagaatct
    16801 tgcgtagggt catggtgttt ccttcttatg atatgcaggt gatccgacca cttgggtctg
    16861 agttttaatc atatgtgcta tttatcgaat ttcccgcaag tgtgatgcca gtttgcggtc
    16921 aagcgcacaa atcatatgaa aaatgaatgc ttatactgaa gacggcgctt cggtaaaaag
    16981 ataattctga ataattgtaa cctttaggta aaaaaagtta tacgcggtgg aaacattgcc
    17041 cggatagtct atagtcacta agcattaaaa tttgcgcctc ataatagttg ggccgattgt
    17101 ggcaccgcac aggcgtaata ctcagcagga gataacaatg aaaattttcc aacggtacaa
    17161 cccacttcag gtggcgaagt acgtaaagat cctgttccgt ggacggttat acatcaagga
    17221 cgttggcgct tttgaatttg ataagggtaa gattcttatc ccaaaagtga aggataagct
    17281 gcatttgtca gtgatgtccg aagttaaccg tcaggttatg cgtctgcaaa cagagatggc
    17341 ttaaccaaag tgctatgcag taataagacg gctcctgatt caggagccgt tgatgtttct
    17401 gggggttacg cgacgctttc ttctttatct ttagcgtcag gcaattttgg cgaaagggcg
    17461 gtggccttgc tgtcgattcg ggtcaccagc agctggtcga tgcggtagtt atcgatatcc
    17521 accacttcaa atttgtagcc ggcgaatttc accgaatcgg tgcgtttcgg gatcttacgc
    17581 agcataaaca tcataaagcc gccgatggtt tcgtagttgc ccgactgcgg gaactcgtca
    17641 atatccagca cgcgcatgac gtcgtcaata ggcgtgccgc cgtctatcag ccatgagttc
    17701 tcatcacggg cgacaatttg ctcttccagc ccctgaccga ccagatcgcc catcagcgtg
    17761 gtcatcacgt cgttgagggt gatgatcccc actaccagcg cgtactcgtt catgatcacc
    17821 gcgaagtctt cacctgcggt tttaaaactt tccaacgcct ctgaaagggt taacgtatcc
    17881 ggcacaatca gcgtattgcg aatttgtacg ccgctgttca gtgccaggct ttggttagcc
    17941 agcacgcggt tcagcaggtc tttagaatcg acatagccga tgatgtggtc aatatcttca
    18001 ttacagacga gaaacttaga gtgcggatgt tccgccacct tattcttcag actttgctca
    18061 tcttcgtgga gatcaaacca aatcacgttt tcacgcggtg tcattgaaga cggaacggta
    18121 cgggattcca gctcaaagac gttttcaatc agctcgtgtt cctgtttacg taacacgccc
    18181 gccagcgcac cggcttccac taccgcgtag atgtcatcag aagtgatatc atctttacgt
    18241 accattggca gtttgaaaat acggaagatt atgttcgcca gtccgttgaa gaaccacacc
    18301 agcggggtgc aaacgtacag gcagaagcgc atcgggttga tgatacgcaa agccacagct
    18361 tctggcgcaa tcataccgat gcgtttcggg gttaaatccg caaacaggat aaacatgcca
    18421 gtcactaacg agaaagagag aataaagctc agttgctcag agagttctgc cgacatatag
    18481 cgggagaaca ggctgtgaaa agctggagaa aatgccgcat caccgacgat accgccgaga
    18541 atcgccactg cgttcagacc gatctggacc acggtaaaga acatgccggg attttcctgc
    18601 atattcagaa cgcgttgggc atttatattg ccttcatcag ccagcagttt aagtttgatt
    18661 ttgcgtgagg cggcaagcga gatctcggac atcgagaaga acgcacttac agcgatcaaa
    18721 cagagtatga ctaaaatact gtttaacata tcttatccga cctttcaggc cagatcctcg
    18781 gaaggggaag ttgattaatt tgtgtgaaat acacattgaa agccgattcg aagaaagtga
    18841 accggcattt tcagtggcta gtatagcgta aggtactgta aagccgccag agggttaaaa
    18901 ttcagacagc tgaaaaatga aaaactgcct gatgcgctat gccgaacagg cctacgcagt
    18961 ccccgcaata tattgaattt ggacgatttt gtaggcctgg caaggcgttt acgccgtatc
    19021 cggcataaac aaaatgcact ttatcaacaa tctacatttg tacccgtaac gctatgcttc
    19081 cggcggcaga cagacgccaa ttccgccaat tccacagtaa ccatacgggt ttttatgcag
    19141 atattgctgg tggtcatctt cggcataata aaacggtgtg gcgttagcga tttccgtggt
    19201 gatgcgacga tcgtcatcgg cggcaagcat cgccgcctga aaacgttcca gactggcgcg
    19261 agcggcggca tcctgttctg gggtcagcgg ataaatcgct gaacgatact gcgtgccgtg
    19321 gtcattgccc tgacgcattc cctgggcggg atcgtgattc tcccaaaata cttgtagcaa
    19381 ctgctcatag ctgatgacgg atggatcgta aacaatgcgc accgcttcgg catggccagt
    19441 atcgccggag cacacttccc gataagtcgg atttggcgta tagccgccgg tatagcctgc
    19501 ggcggtgctg taaacgccgg gtaactgcca gaacagacgc tccacacccc agaaacaacc
    19561 catcgcaaaa atggcaatct ccattccgtc aggtacattg gtcattgagt gaccgttgac
    19621 cgcatgcagt gtggctacgg gcatcggggt gttacgtcca ggcagggcat cggcggggga
    19681 aaccagatgc tttttatcaa ataaactcat ggtgtcgctc tcccgaaaat cggtcattgg
    19741 ggttaaggtt gtaacaagag gcgtatttgc acacaataac caccgtgaat aggtctaaag
    19801 taaaacataa gaaatatttt gggtttagtc tgctttttaa tccatattac tggatttttg
    19861 ttaagccgtt taacggcgtt ccaggggcag gaaaaaagga tattcaggag aaaatgtgcg
    19921 ctatatccga cagttatgct gtgtaagctt actctgctta agcggatctg ccgtcgccgc
    19981 gaacgtccgt ctacaggtcg aggggttatc gggacagctg gaaaagaacg ttcgcgcgca
    20041 gctttctacg attgaaagtg atgaagtgac gccagaccgt cgctttcgcg cacgcgtcga
    20101 tgatgccatc cgcgaaggtc tgaaagcgct gggctattac cagccgacca ttgaatttga
    20161 tctccgtcca ccgccaaaga aagggcggca ggtattgatc gccaaagtca cgccaggcgt
    20221 gccggtgtta attggcggca ccgatgtggt attgcgcggc ggcgcgcgga ccgataaaga
    20281 ctatttgaaa ttgctcgata ctcgcccggc tattggtacg gtactgaacc agggcgatta
    20341 tgaaaatttc aaaaagtcct taaccagcat tgcgttgcgt aaaggttatt tcgatagcga
    20401 atttaccaaa gcgcagctgg gcattgcgct cggcctgcat aaagccttct gggatattga
    20461 ttataacagt ggcgaacgtt accgctttgg gcatgtgacc tttgaaggat cacaaatccg
    20521 cgatgaatac ctgcaaaatc tggtgccgtt taaagagggc gatgagtacg aatcgaaaga
    20581 tctggcagaa ctgaaccgtc gactttctgc taccggctgg tttaactcgg tggtggtggc
    20641 tccacaattt gataaagcgc gcgaaacgaa agtattacca ttgacgggcg tggtttcgcc
    20701 gcgaacagaa aacaccatcg aaaccggcgt cggttactct acggacgtgg gaccgcgcgt
    20761 gaaagcgacg tggaaaaagc cgtggatgaa ctcttacggt cacagtttga ccaccagtac
    20821 cagtatttcc gcgccggaac agatcctcga cttcagctat aaaatgccgc tgctgaagaa
    20881 tccgctggaa caatattatt tggtgcaggg cggttttaag cgcactgacc tgaacgatac
    20941 cgagtctgac tccactacgc tggtggcttc tcgctactgg gatctctcca gcggctggca
    21001 gcgtgccatt aacctgcgct ggagtttcga ccactttacc cagggtgaaa ttaccaacac
    21061 cacgatgctg ttttatcctg gggtgatgat tagccgcacg cgttctcgtg gtggcctgat
    21121 gccaacctgg ggcgactcgc aacgctactc tatcgactac tccaacacgg cctggggctc
    21181 ggatgttgat ttctccgttt tccaggcgca gaacgtctgg atccgcacac tgtacgatcg
    21241 ccatcgtttt gtgacacgcg gcacgctggg ctggattgaa acgggtgatt tcgacaaagt
    21301 accgccggat ctgcgtttct tcgccggggg cgatcgcagt attcgcggct acaaatacaa
    21361 atctatcgcc ccgaaatacg ccaacggtga cctgaaaggg gcctcgaagc tgataaccgg
    21421 atcgctggaa taccagtaca acgtgaccgg aaaatggtgg ggcgcggtgt ttgtcgatag
    21481 cggcgaagcg gtaagcgata ttcgccgcag cgactttaaa accggtaccg gggtcggcgt
    21541 gcgctgggaa tcgccggtcg ggccaatcaa actcgatttt gccgtaccgg tcgcggataa
    21601 agacgaacac gggttacagt tttacatcgg tctggggcca gaattatgag tttatggaaa
    21661 aaaatcagcc tcggcgtggt tatcgttatc ttactgttgc tgggatcggt ggcgtttctg
    21721 gtgggcacca ccagcggcct gcatctggta tttaaagcgg cggatcgctg ggtgccagga
    21781 ctggatattg gcaaggtcac cggcggctgg cgtgatctca ccttgtctga cgttcgttat
    21841 gagcagccag gcgtggcggt aaaagcgggc aatctgcatc tggcggtcgg gcttgagtgc
    21901 ctgtggaaca gtagcgtttg tattaatgat ctggcgctga aagacattca ggtcaacatc
    21961 gacagtaaaa aaatgcctcc ttctgaacag gttgaagaag aggaagatag cggtccgttg
    22021 gatctctcca tgccgtatcc cattaccctg acacgggtgg cgctggacaa cgtcaacatc
    22081 aagattgatg acaccacggt atcggtgatg gacttcacct ccggcctgaa ctggcaggag
    22141 aaaaccctga ccctgaaacc gacatcgctg aaaggcctgt tgattgcttt gccgaaagtg
    22201 gcggaagcgg cgcaggaaga agtggtcgaa ccgaaaattg aaaatccaca gccggaggaa
    22261 aagccgctcg gcgaaacgct gaaagatctc ttttctcgcc cggtattgcc ggaaatgacc
    22321 gacgtgcatc tgccgctcaa cctgaacatt gaagagttta agggcgagca actgcgcgtg
    22381 acgggcgaca cggacatcac cgtgcgcacc atgctgctga aagtgagcag tatcgacggc
    22441 aataccaaac tggacgcgct ggatatcgac tccaaccagg ggatcgtcaa cgccagcggc
    22501 acggcgcagc tgtcagacaa ctggccggtg gatatcaccc tcaacagcac actgaacgtg
    22561 gagccgttga aaggtgaaaa ggtgaagctg aaagtgggcg gtgcgctgcg cgaacagctg
    22621 gagataggcg ttaacctttc cggtccggtg gatatggatt tacgcgccca gacgcgactg
    22681 gcggaagccg gattgccgct caacgtggaa gtgaacagca aacagcttta ctggccgttc
    22741 actggtgaga agcagtatca ggcggatgat ctgaaactga aacttaccgg caaaatgacc
    22801 gattatgcga tctctatgcg cacggctgtt aagggccagg agatcccgcc agccaccatt
    22861 acccttgatg ccaaaggtaa tgaacagcag gtcaatctcg acaaacttac cgtcgcggcg
    22921 ctggaaggga aaacggaact taaggcgttg ctcgactggc agcaggccat tagttggcgc
    22981 ggtgagctaa cgcttaacgg tattaacacc gccaaagaga tcccggagtg gccgtcgaaa
    23041 ctcaatggct tgattaaaac ccgcggtagc ctgtacggcg gcacctggca gatggaggtg
    23101 ccagaactga agctgaccgg taacgtcaaa cagaacaaag tgaacgttga cggcacgctg
    23161 aaaggcaaca gttatatgca gtggatgatc ccagggctgc atctggaact ggggccaaac
    23221 agtgccgaag tgaaaggcga gctgggggta aaagatctca atcttgatgc caccatcaac
    23281 gcgccggggc tggataacgc gctgccgggg cttggcggta cagcgaaagg gttggtgaaa
    23341 gtacgcggca cggtggaagc gccacaacta ctggcagata tcaccgcgcg cggcctgcgc
    23401 tggcaggaac tttccgtggc gcaggttcgc gtggaaggcg atatcaaatc caccgaccag
    23461 attgcgggta aactcgacgt acgcgttgag caaatttcgc agccggatgt aaatatcaac
    23521 ctcgtcaccc tgaatgccaa aggcagcgaa aagcagcacg agctacagtt gcggattcag
    23581 ggcgagcctg tctccgggca gcttaatctg gcaggaagtt ttgatcgcaa agaagaacgc
    23641 tggaagggaa ctcttagcaa tacccgcttc cagacgccgg tcggcccgtg gtcgctgacc
    23701 cgcgatattg cgctggatta ccgcaataag gagcaaaaaa tcagcatcgg gccacactgc
    23761 tggcttaacc cgaatgcgga actgtgcgtg ccgcaaacta tcgatgcggg tgccgaaggg
    23821 cgtgcggtgg tgaatctcaa ccgcttcgac ctggccatgc tgaaaccatt tatgccagaa
    23881 accactcagg ccagcggtat cttcacgggt aaagcggacg ttgcctggga caccacgaaa
    23941 gaggggctgc cgcagggcag tatcaccctt tcggggcgta atgtgcaggt gacgcaaacc
    24001 gtcaacgatg cggcgctgcc cgtggcgttt cagacgctga atctgacggc ggaattgcgt
    24061 aacaaccgtg ccgaattggg ctggaccatc cgcctgacca ataacggcca gtttgatgga
    24121 caggtgcagg tgaccgatcc gcaaggccgc cgtaatcttg gtggcaacgt caatatccgt
    24181 aacttcaacc ttgcgatgat aaaccccatc tttacccgtg gggaaaaagc agcggggatg
    24241 gtgagtgcca acttgcgtct gggtggtgat gtgcaaagcc cgcagttgtt tggtcagctt
    24301 caggttacgg gtgtggatat cgacggcaac tttatgccgt ttgatatgca accgagccag
    24361 cttgcggtca actttaacgg tatgcgctcg acgcttgccg gtacagtacg gacccagcag
    24421 ggtgaaatct acctgaacgg tgatgccgac tggagccaga ttgaaaactg gcgggcgcgg
    24481 gtaacggcga aaggcagtaa agtgcggatc accgtgccgc cgatggtacg aatggatgta
    24541 tcgccagatg ttgtattcga ggctacacca aacctgttta ccctcgatgg tcgcgtggat
    24601 gtcccgtggg cgcgcatcgt ggtgcatgag ctgccggaaa gcgcggtggg cgtctccagc
    24661 gatgtggtaa tgcttaacga taacctgcaa ccggaagagg caaaaacggc gtcgattccg
    24721 attaacagca acctgattgt ccacgtcggc aacaatgtgc gcattgacgc ctttggcctg
    24781 aaagcgcggc tgacgggcga tctcaacgtc gttcaggaca aacaagggct gggcctgaac
    24841 gggcagatca acatccctga agggcgcttc catgcctatg gtcaggatct gattgtgcgt
    24901 aagggtgagt tactgttctc tggtccgccg gatcaaccgt atcttaatat cgaagctatt
    24961 cgtaacccgg atgctacaga agacgacgta atcgccggag ttcgcgtcac tggtctggcg
    25021 gacgaaccga aagcggagat cttctctgac ccggccatgt cgcaacaggc tgccttgtct
    25081 tatttgctac gtggacaagg gctggagagc gatcagagcg acagtgcggc gatgacctcg
    25141 atgctgattg gtctgggggt tgcacaaagt ggtcagattg tgggtaaaat cggcgagacg
    25201 tttggcgtaa gcaatttagc gctcgacacc cagggagtag gcgactcctc ccaggtggtg
    25261 gtcagcggct atgtattgcc aggtctgcaa gtgaaatacg gcgtgggtat atttgactct
    25321 atagcaacac tcacgttacg ttatcgcctg atgcctaagc tatatctgga agccgtgtct
    25381 ggtgtagacc aggcactgga tttgctctat cagttcgagt tttagcaatg cgaatatttg
    25441 tctacggcag tttacgccac aaacaaggca acagtcactg gatgaccaat gcccagttac
    25501 tgggcgattt cagtatcgat aactaccagt tgtatagcct gggccactat ccaggcgcag
    25561 ttccggggaa cggaacagta cacggtgaag tttatcgtat tgataacgcc acgctggccg
    25621 aacttgatgc cttgcgcacc aggggcggtg aatacgcgcg ccagttgatt cagacgccgt
    25681 acgggagtgc atggatgtac gtttatcaac gacccgtcga tggattaaag ctaattgaaa
    25741 gcggcgactg gttagacagg gataagtaat aataaaagca cgccaccttc gggtggcgcg
    25801 aaaattgatg taatgcaatg aaaaataaag gattattttt ctcgttgtcc acattttgcc
    25861 atagcgatac atcttagccc gtaatgattc gcgggctaag catatacaaa atgttaagcc
    25921 tgatacgggg gtcctatgtt tgatagggaa atgataaacg aaaataaaga ggcgtttttt
    25981 ctgtatgtta acggtagagg ggagttgtcg gctcaatgcg tgataaaggt ggctgaaaaa
    26041 ggggattatt tacaaggatt ctctttaact cgtcgcgcat ttcgtacctt tttgaaagac
    26101 cgtgttattc agatgttttc tagcgaccag gagttgaata aggttgcgtc tgaaac
//
LOCUS       DAZFLD010000053        25992 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.053, whole genome shotgun sequence.
ACCESSION   DAZFLD010000053 DAZFLD010000000
VERSION     DAZFLD010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25992
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.053"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(15..971)
                     /gene="rpnC"
                     /locus_tag="ACHKBL_001845"
     CDS             complement(15..971)
                     /gene="rpnC"
                     /locus_tag="ACHKBL_001845"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004536 - DNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HFO5818724.1"
                     /translation="MDAPSTTPHDAVFKQFLMHAETARDFLDIHLPAELRELCDLDTL
                     HLESGSFIEESLKGHSTDVLYSVQMQGNPGYLHVVIEHQSKPDKKMTFRMMRYAIAAM
                     HRHLEADHDKLPLVVPILFYQGEATPYPLSMCWFDMFYSPELARRVYNNPFPLVDITI
                     TPDDEIMQHRRIAILELLQKHIRQRDLMLLLEQLVTLIDEGYTSGSQLVAMQNYMLQR
                     GHTEQADLFYGVLRDRETGGESMMTLAQWFEEKGRQEERQEVRQEVRQEVRQEVRQEV
                     IQEVRQEVRQEYALRFLSKGMSREDVAEMANLPLAEVDKLIN"
     gene            1245..1625
                     /gene="panD"
                     /locus_tag="ACHKBL_001846"
     CDS             1245..1625
                     /gene="panD"
                     /locus_tag="ACHKBL_001846"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HFO5818725.1"
                     /translation="MIRTMLQGKLHRVKVTHADLHYEGSCAIDQDFLDAAGILENEAI
                     DIWNVTNGKRFSTYAIAAERGSRIISVNGAAAHCASVGDIVIIASFVTMPDEEARTWR
                     PNVAYFEGDNEMKRTAKAIPVQVA"
     gene            complement(1629..2858)
                     /gene="yadE"
                     /locus_tag="ACHKBL_001847"
     CDS             complement(1629..2858)
                     /gene="yadE"
                     /locus_tag="ACHKBL_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFO5818726.1"
                     /translation="MYKQAVILLLMLFTASVSAALPARYMQTIENAAVWAQIGDKMVT
                     VGNIRAGQIIAVEPTAASYYAFNFGFGKGFIDKGHLEPVQGRQKVEDGLGDLNKPLSN
                     QNLVTWKDTPVYNAPSAGSAPFGVLADNLRYPILHKLKDRLNQTWYQIRIGDRLAYIS
                     ALDAQPDNGLPVLTYHHILRDEENTRFRHTSTTTSVRAFNNQMAWLRDRGYTTLSMVQ
                     LEGYVKNKINLPARAVVITFDDGLKSVSRYAYPVLKQYGMKATAFIVTSRIKRHPQKW
                     DPKSLQFMSVSELNEIRDVFDFQSHTHFLHRVDGYRRPILLSRSEHNILFDFARSRRA
                     LAQFNPHVWYLSYPFGGFNDKAVKAAKEAGFHLAVTTMKGKVKPGDNPLLLKRLYILR
                     TDSLETMSRLVSNQPQG"
     gene            complement(2922..3362)
                     /gene="yadI"
                     /locus_tag="ACHKBL_001848"
     CDS             complement(2922..3362)
                     /gene="yadI"
                     /locus_tag="ACHKBL_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFO5818727.1"
                     /translation="MLGWVITCHDDRAQEILDALEKKHGALLQCRAVNFWRGLSSNML
                     SRMMCDALHEADSGEGVIFLTDIAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMLV
                     YRETLSSAEFRERIVELGAPEVSSLWHQQQKNPPFVLKHNLYEY"
     gene            complement(3467..4237)
                     /gene="yadH"
                     /locus_tag="ACHKBL_001849"
     CDS             complement(3467..4237)
                     /gene="yadH"
                     /locus_tag="ACHKBL_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO5818728.1"
                     /translation="MMHLYWVALKSIWAKEIHRFMRIWVQTLVPPVITMTLYFIIFGN
                     LIGSRIGDMHGFSYMQFIVPGLIMMSVITNAYANVASSFFGAKFQRNIEELLVAPVPT
                     HVIIAGYVGGGVARGLFVGILVTAISLFFVPFQVHSWVFVALTLVLTAVLFSLAGLLN
                     GVFAKTFDDISLVPTFVLTPLTYLGGVFYSLTLLPPFWQGLSHLNPIVYMISGFRYGF
                     LGINDVPLVTTFGVLVVFIVAFYLICWSLIQRGRGLRS"
     gene            complement(4234..5160)
                     /gene="yadG"
                     /locus_tag="ACHKBL_001850"
     CDS             complement(4234..5160)
                     /gene="yadG"
                     /locus_tag="ACHKBL_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO5818729.1"
                     /translation="MTIALELQQLKKTYPGGVQALRGIDLQVEAGDFYALLGPNGAGK
                     STTIGIISSLVNKTSGRVSVFGYDLEKDVVNAKRQLGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVERKEAYIRSEKYLKQLDLWGKRNERARMLSGGMKRRLMIARALMHEPKLLIL
                     DEPTAGVDIELRRSMWGFLKDLNDKGTTIILTTHYLEEAEMLCRNIGIIQHGELVENT
                     SMKALLAKLKSETFILDLAPKSPLPKLDGYQYRLVDTATLEVEVLREQGINSVFTQLS
                     EQGIQVLSMRNKANRLEELFVSLVNEKQGDRA"
     gene            5269..5931
                     /gene="can"
                     /locus_tag="ACHKBL_001851"
     CDS             5269..5931
                     /gene="can"
                     /locus_tag="ACHKBL_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HFO5818730.1"
                     /translation="MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPRFLWIGCSD
                     SRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVVQYAVDVLEVEHIIICGHYGCG
                     GVQAAVENPELGLINNWLLHIRDIWFKHSSLLGEMPQERRLDTLCELNVMEQVYNLGH
                     STIMQSAWKRGQKVTIHGWAYGIHDGLLRDLDVTATNRETLEQRYRHGISNLKLKHAN
                     HK"
     gene            complement(5972..6508)
                     /gene="hpt"
                     /locus_tag="ACHKBL_001852"
     CDS             complement(5972..6508)
                     /gene="hpt"
                     /locus_tag="ACHKBL_001852"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004204384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HFO5818731.1"
                     /translation="MKHTVEVMIPEAEIKARIAELGRQITERYKDSGSDMVLVGLLRG
                     SFMFMADLCREVQVSHEVDFMTASSYGSGMSTTRDVKILKDLDEDIRGKDVLIVEDII
                     DSGNTLSKVREILSLREPKSLAICTLLDKPSRREVNVPVEFIGFSIPDEFVVGYGIDY
                     AQRYRHLPYIGKVILLDE"
     gene            6714..9104
                     /gene="gcd"
                     /locus_tag="ACHKBL_001853"
     CDS             6714..9104
                     /gene="gcd"
                     /locus_tag="ACHKBL_001853"
                     /EC_number="1.1.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein glucose dehydrogenase"
                     /protein_id="HFO5818732.1"
                     /translation="MAINNTGSRRLLVTLTALFAALCGLYLLIGGGWLVAIGGSWYYP
                     IAGLVMLGVAWMLWRSKRAALWLYAALLLGTMIWGVWEVGFDFWALTPRSDILVFFGI
                     WLILPFVWRRLVIPASGAVAALVVALLISGGILAWAGFNDPQEISGTLSADTTPAEAI
                     SPVADQDWPAYGRNQEGQRFSPLKQINADNVHKLKEAWVFRTGDVKQPNDPGEITNEV
                     TPIKVGDTLYLCTAHQRLFALDAASGKEKWHYDPELKTNESFQHVTCRGVSYHEAKAE
                     TASPEVMADCPRRIILPVNDGRLIAINAENGKLCETFANKGVLNLQSNMPDTKPGLYE
                     PTSPPIITDKTIVMAGSVTDNFSTRETSGVIRGFDVNTGELLWAFDPGAKDPNAIPSD
                     EHTFTFNSPNSWAPAAYDAKLDLVYLPMGVTTPDIWGGNRTPEQERYASSILALNATT
                     GKLAWSYQTVHHDLWDMDLPAQPTLADITVNGQKVPVIYAPAKTGNIFVLDRRNGELV
                     VPAPEKPVPQGAAKGDYVTPTQPFSELSFRPTKNLSGADMWGATMFDQLVCRVMFHQM
                     RYEGIFTPPSEQGTLVFPGNLGMFEWGGISVDPNREVAIANPMALPFVSKLIPRGPGN
                     PMEQPKDAKGTGTESGIQPQYGVPYGVTLNPFLSPFGLPCKQPAWGYISALDLKTNEV
                     VWKKRIGTPQDSMPFPMPVPVPFNMGMPMLGGPISTAGNVLFIAATADNYLRAYNMSN
                     GEKLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGTKMGDYIVAYALPDDVK"
     gene            complement(9300..10850)
                     /gene="cueO"
                     /locus_tag="ACHKBL_001854"
     CDS             complement(9300..10850)
                     /gene="cueO"
                     /locus_tag="ACHKBL_001854"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase CueO"
                     /protein_id="HFO5818733.1"
                     /translation="MQRRDFLKYSVALGVASALPLWSRAVFAAERPTLPIPDLLTTDA
                     RNRIQLTIGAGQSTFGGKTATTWGYNGNLLGPAVKLQRGKAVTVDIYNQLTEETTLHW
                     HGLEVPGEVDGGPQGIIPPGGKRSVTLNVDQPAATCWFHPHQHGKTGRQVAMGLAGLV
                     VIEDDEILKLMLPKQWGIDDVPVIVQDKKFNADGQIDYQLDVMTAAVGWFGDTLLTNG
                     VIYPQHAAPRGWLRLRLLNGCNARSLNFATSDNRPLYVIASDGGLLPEPVKVNELPVL
                     MGERFEVLVEVNDNKPFDLVTLPVSQMGMAIAPFDKPHPVMRIQPIAISASGALPDTL
                     SSLPALPSLEGLTVRKLQLSMDPMLDMMGMQMLMEKYGDQAMAGMDHSQMMGHMGHGN
                     MNHMNHGGKFDFHHANKINGQAFDMNKPMFAAAKGQYERWVISGVGDMMLHPFHIHGT
                     QFRILSENGKPPATHRAGWKDTVKVEGNVSEVLVKFNHDAPKEHAYMAHCHLLEHEDT
                     GMMLGFTV"
     gene            11016..11363
                     /gene="yacC"
                     /locus_tag="ACHKBL_001855"
     CDS             11016..11363
                     /gene="yacC"
                     /locus_tag="ACHKBL_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacC family pilotin-like protein"
                     /protein_id="HFO5818734.1"
                     /translation="MKTFFRTVLFGSLMAVCANSYALSESEAEDMADLTAVFVFLKND
                     CGYQNLPNGQIRRALVFFAQQNQWDLSNYDTFDMKALGEDSYRDLSGIGIPVAKKCKA
                     LARDSLSLLAYVK"
     gene            11469..12335
                     /gene="speE"
                     /locus_tag="ACHKBL_001856"
     CDS             11469..12335
                     /gene="speE"
                     /locus_tag="ACHKBL_001856"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HFO5818735.1"
                     /translation="MAEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFG
                     RVMALDGVVQTTERDEFIYHEMMTHVPLLAHGHAKHVLIIGGGDGAMLREVTRHKNVE
                     SITMVEIDAGVVSFCRQYLPNHNAGSYDDPRFKLVIDDGVNFVNQTSQTFDVIISDCT
                     DPIGPGESLFTSAFYEGCKRCLNPGGIFVAQNGVCFLQQEEAIDSHRKLSHYFSDVGF
                     YQAAIPTYYGGIMTFAWATDNDALRHLSTEIIQARFLASGLKCRYYNPAVHTAAFALP
                     QYLQDALASQPS"
     gene            12351..13145
                     /gene="speD"
                     /locus_tag="ACHKBL_001857"
     CDS             12351..13145
                     /gene="speD"
                     /locus_tag="ACHKBL_001857"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HFO5818736.1"
                     /translation="MKKLKLHGFNNLTKSLSFCIYDICYAKTTEERDGYIAYIDELYN
                     ANRLTEILSETCSIIGANILNIARQDYEPQGASVTILVSEEPVDPKLIDKTEHPGPLP
                     ETVVAHLDKSHICVHTYPESHPEGGLCTFRADIEVSTCGVISPLKALNYLIHQLESDI
                     VTIDYRVRGFTRDINGMKHFIDHEINSIQNFMSDDMKALYDMVDVNVYQENIFHTKML
                     LKEFDLKHYMFHTKPEDLTDSERQEITTALWKEMREIYYGRNMPAV"
     gene            complement(13183..13545)
                     /gene="yacL"
                     /locus_tag="ACHKBL_001858"
     CDS             complement(13183..13545)
                     /gene="yacL"
                     /locus_tag="ACHKBL_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YacL"
                     /protein_id="HFO5818737.1"
                     /translation="MDYEFLRDITGVVKVRMSMGHEVVGHWFNEEVKENLALLDEVEQ
                     AAHALKGSERSWQRAGHEYTLWMDGEEVMVRANQLEFAGDEMEEGMNYYDEESLSLCG
                     VEDFLQVVAAYRNFVQQK"
     gene            complement(13720..16317)
                     /gene="acnB"
                     /locus_tag="ACHKBL_001859"
     CDS             complement(13720..16317)
                     /gene="acnB"
                     /locus_tag="ACHKBL_001859"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019080489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HFO5818738.1"
                     /translation="MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEEEF
                     LLDLLTNRVPPGVDEAAYVKAGFLAAIAKGEAKSPLLTPEKAIELLGTMQGGYNIHPL
                     IDALDDAKLAPIAAKALSHTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFLNRP
                     ALAEKLTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVG
                     PIKQIEALQQKGFPLAYVGDVVGTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGG
                     KIAPIFFNTMEDAGALPIEVDVSNLNMGDVIDVYPYKGEVRNHETGELLATFELKTDV
                     LIDEVRAGGRIPLIIGRGLTTKAREALGLPHSDVFRQAKDVAESDRGFSLAQKMVGRA
                     CGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
                     PVDVNTHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISF
                     PAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLYAIKQGLLTVE
                     KKGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTIKLNKEPIIEYLNSNI
                     VLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYAAVIDIDLADIKEPIL
                     CAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAAGKLLDAHKGQLPTRLWVAPP
                     TRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLG
                     TGANVFLASAELAAVAALIGKLPTPEEYQTYVAQVDKTAVDTYRYLNFDQLSQYTEKA
                     DGVIFQTAV"
     gene            16672..18429
                     /gene="yacH"
                     /locus_tag="ACHKBL_001860"
     CDS             16672..18429
                     /gene="yacH"
                     /locus_tag="ACHKBL_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HFO5818739.1"
                     /translation="MKMTLPFKPHVLALICSAGLCAASTGLYIKSRTVEAPVEPQSTQ
                     LAAPDISAVTLPATVSVPPVTPAVVKSAFSTAQIDQWVAPVALYPDSLLSQVLMASTY
                     PANVAQAVQWSHDNPLKQGDAAIQAVSDQPWDASVKSLVAFPQLMALMGENPQWVQNL
                     GDAFLAQPQEVMDSVQRLRQLAQQTGSLKSSTEQKVITTTKKAVPVKQTVTAPVIPSN
                     TVSTANPVITEPATTVISIEPTNPDVVYIPNYNPTVVYGNWANTAYPPVYLPPPAGEP
                     FVDSFVRGFGYSMGVATTYALFSSIDWDDDDHDHHHHDDDDYHHHDGGHRDGNGWQHN
                     GDNINIDVNNFNRITGEHLTDKNMAWRHNPNYRNGVPYHDQDMAKRFHQTDVNGGMSA
                     TQLPAPTRDSQRQAAASQFQQRTHSAPVITRDTQRQAAAQRFNEAENYGSYDDFRDFS
                     RRQPLTQQQKDAARQRYQSASPEQRQAVREKMQTNPQIQQRREAARERIQSATPEQRQ
                     VFKEKVQQRPLNQQQRDNARQRVQSASPEQRQVFREKVQESRPQRLNDSDHTVRLNNE
                     QRSAVRERLSERGARRLER"
     gene            complement(18500..19924)
                     /gene="lpdA"
                     /locus_tag="ACHKBL_001861"
     CDS             complement(18500..19924)
                     /gene="lpdA"
                     /locus_tag="ACHKBL_001861"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFO5818740.1"
                     /translation="MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGV
                     CLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLA
                     GMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
                     RIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDI
                     VKVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPN
                     GKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDC
                     ADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNPKAKKK"
     gene            complement(20249..22141)
                     /gene="aceF"
                     /locus_tag="ACHKBL_001862"
     CDS             complement(20249..22141)
                     /gene="aceF"
                     /locus_tag="ACHKBL_001862"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004742 - dihydrolipoyllysine-residue
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HFO5818741.1"
                     /translation="MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASM
                     EVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGAADAAPAQAEEKKEAAPAAAPAA
                     AAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
                     KVNVGDKVSTGSLIMVFEVAGEAGAAAPAAKQEAAPAAASAPAAGVKDVNVPDIGGDE
                     VEVTEVMVKVGDKVAAEQSLITVEGDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIM
                     IFEVEGAAPAAAPAKQEAAAPAPAAKAEAPAAAPAAKAEGKSEFAENDAYVHATPLIR
                     RLAREFGVNLAKVKGTGRKGRILREDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKV
                     DFSKFGEIEEVELGRIQKISGANLSRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAK
                     RKLDVKITPVVFIMKAVAAALEQMPRFNSSLSEDGQRLTLKKYINIGVAVDTPNGLVV
                     PVFKDVNKKGIIELSRELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIVN
                     APEVAILGVSKSAMEPVWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSD
                     IRRLVM"
     gene            complement(22156..24819)
                     /gene="aceE"
                     /locus_tag="ACHKBL_001863"
     CDS             complement(22156..24819)
                     /gene="aceE"
                     /locus_tag="ACHKBL_001863"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008814576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HFO5818742.1"
                     /translation="MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEA
                     RKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDL
                     ELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQ
                     EQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLK
                     DTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKII
                     NELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYV
                     REHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHTI
                     KGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHT
                     YLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKEISTTIAFVRALNVMLK
                     NKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYTPQDREQVAYYKEDEKGQIL
                     QEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFL
                     IGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEK
                     QENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVR
                     EAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPA
                     VASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGE
                     LAKRGEIDKKVVADAIAKFNIDADKVNPRLA"
     gene            complement(24980..25744)
                     /gene="pdhR"
                     /locus_tag="ACHKBL_001864"
     CDS             complement(24980..25744)
                     /gene="pdhR"
                     /locus_tag="ACHKBL_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex transcriptional
                     repressor PdhR"
                     /protein_id="HFO5818743.1"
                     /translation="MAYSKIRQPKLSDVIEQQLEFLILEGTLRPGEKLPPERELAKQF
                     DVSRPSLREAIQRLEAKGLLLRRQGGGTFVQSSLWQSFSDPLVELLSDHPESQYDLLE
                     TRHALEGIAAYYAALRSTDEDKERIRELHHAIELAQQSGDLDAESNAVLQYQIAVTEA
                     AHNVVLLHLLRCMEPMLAQNVRQNFELLYSRREMLPLVSSHRTRIFEAIMAGKPEEAR
                     EASHRHLAFIEEILLDRSREESRRERSLRRLEQRKN"
BASE COUNT         6204 a         7120 c         6357 g         6311 t
ORIGIN      
        1 ataatgcaga taacttagtt aatcagctta tcaacctcag caagaggtaa atttgccatc
       61 tctgcaacgt cttcccgaga catcccttta ctcagaaaac gcagggcgta ttcctgtctt
      121 acttcctgtc taacctcttg tattacctcc tgtcttacct cctgtcttac ctcctgtctt
      181 acctcctgcc ttacctcctg cctttcctcc tgtcttccct tctcttcaaa ccactgcgcc
      241 agcgtcatca tagactcccc tcccgtttcc ctgtctctca gcacaccata aaacaaatcc
      301 gcttgttcag tatgaccgcg ttgcagcata tagttttgca tggcaactaa ctgacttccg
      361 ctagtgtacc cttcgtcaat cagcgtgacc agttgctcca gcaataacat taagtcgcgc
      421 tggcgaatat gtttttgcag tagttcgaga atcgcaatcc gccgatgttg catgatttcg
      481 tcatccggtg tgatggtgat atccaccagc gggaaagggt tgttgtaaac gcgtcgcgcc
      541 agctccggcg agtaaaacat atcaaaccag cacattgaga gcggataagg tgtggcctcg
      601 ccctgataaa acagaatcgg caccaccagc ggcagcttat cgtgatccgc ctccagatgc
      661 cggtgcatgg cggcaatggc atagcgcatc atgcgaaagg tcattttctt atccggcttg
      721 ctttgatgtt caatcaccac atgcaaataa ccgggattac cctgcatttg cacggaataa
      781 agcacgtcag tgctgtgccc tttcaggctt tcttcaataa aactccccga ctcaagatgc
      841 agcgtgtcga ggtcacacag ttcgcgtagt tccgctggca aatggatatc cagaaagtca
      901 cgagccgttt ccgcatgcat taaaaattgt ttaaataccg cgtcatgcgg tgtggtactc
      961 ggtgcatcca tcttccctgc cttccgtgtt gtcttgcgtg aaggcagtat ccagtcaagg
     1021 cgcgccgtcg tcagccgcct ctttattgtt taccgagcag cgttcagaga aatttcaatt
     1081 ggttacaggc gttacatggc acgcttcggc acggtacgca aactgctgat gcaaatgccg
     1141 tcagatgagt agacactaaa caaaaatcgg gcaatactgc gtgagaattt cctcaaagca
     1201 ggcatcgcct gcttcgttaa cgacagggta gaaaggtaga agttatgatt cgcacgatgc
     1261 tgcagggcaa actccaccgc gtgaaagtga ctcatgcgga cctgcactat gaaggttctt
     1321 gcgccattga ccaggatttt cttgacgcag ctggtattct cgaaaacgaa gccattgata
     1381 tctggaatgt gaccaacggc aagcgtttct ccacctatgc catcgcggca gaacgcggtt
     1441 cgagaattat ttccgttaac ggtgcggcgg cccactgcgc cagcgtcggc gatattgtca
     1501 tcatcgccag cttcgttact atgccagatg aagaagctcg cacctggcga cccaacgtcg
     1561 cctattttga aggcgacaat gaaatgaaac gtaccgcgaa agcgattccg gtacaggttg
     1621 cctgataatc acccctgcgg ctggttactc accagccgcg acatcgtctc gagcgaatcc
     1681 gttcttaaga tataaagtcg ttttagtaac aacggattat cccccggttt tactttgcct
     1741 ttcatggttg tcaccgccag gtgaaatccg gcttcttttg ctgccttcac ggctttgtca
     1801 ttaaatccgc caaacggata cgaaagatac cagacatgcg gattaaattg cgccagagcg
     1861 cggcgtgaac gtgcaaaatc aaacagaata ttgtgctcac tacggctcag caatatgggt
     1921 cggcgataac catccacccg atgcaaaaaa tgggtatgtg actggaaatc aaatacatcg
     1981 cgaatttcgt taagttcaga aacgctcata aattgcagcg attttgggtc ccacttctgc
     2041 gggtgacgtt tgatgcgcga ggtaacaata aacgccgtcg ccttcatgcc atattgtttc
     2101 aacacaggat acgcatagcg gctcaccgat ttgaggccat catcaaaggt aatcaccacc
     2161 gctcgcgcag ggagattgat cttattcttc acgtaacctt ccagctgcac catactcagt
     2221 gtcgtgtatc ccctgtcgcg cagccaggcc atctggttat tgaaagcgcg taccgaggtg
     2281 gtcgtcgaag tatggcgaaa acgggtgttt tcttcgtcgc gcagaatatg gtgataggtt
     2341 agcaccggca ggccattatc gggttgggca tccagcgcgc tgatataggc cagtcgatcg
     2401 ccaatacgga tctgatacca ggtttgattt aacctgtctt tcagtttatg caaaatcggg
     2461 tagcgcaaat tgtccgccag taccccaaac ggcgcactgc ccgcactcgg cgcgttatag
     2521 accggcgtat ctttccaggt aactaagttc tgattactca gcggcttgtt gaggtcaccc
     2581 aaaccgtctt caactttttg tcgcccctga accggctcga gatgaccttt atcgataaac
     2641 cctttaccaa agccaaaatt aaatgcgtaa taacttgcgg cagtgggctc cacggcaatg
     2701 atttgcccgg cccgaatatt ccccacggtc accatcttgt cgccaatttg cgcccagacc
     2761 gcagcatttt cgatggtttg catataacgg gcaggtaacg cggcactgac gcttgcggta
     2821 aacagcatca gcaggagaat aacagcttgt ttgtacatga aaaaataacc gagggtgaaa
     2881 caacgctttt attgtagcaa aagcgtcatc agaatcgcgg gttaatactc atacaaatta
     2941 tgtttgagga cgaaaggcgg atttttttgt tgctggtgcc aaagactact cacctccggc
     3001 gcacccagtt cgacaatacg ctcgcgaaac tctgcgctgg ataacgtttc acggtagacc
     3061 agcatctgct caattaacgg taacgtgacg ccagaaatca cttcgcaacg ggaatgttta
     3121 tggcttaata gcgaagccac gcggtacggt ggcgctccgg ctatatcggt taagaagatg
     3181 acaccttcac cagagtccgc ttcatgcaga gcatcgcaca tcatgcggct gagcatatta
     3241 gagcttaacc cacgccagaa attcacggcc cggcactgaa gaagtgcccc atgttttttc
     3301 tccagcgcat ccagtatctc ttgcgcccgg tcatcgtgac aggtaattac ccaacctaac
     3361 atcgccttcc tccttagccg aaaatcagtc taccctgacc attgttgata aagggtgata
     3421 gccgtcaaaa ctcctctccc ccaaatccag aggagaggaa atagccttag ctacgcaaac
     3481 cacgtccacg ttggatcagc gaccaacaga tcaaataaaa cgccacaata aagaccacca
     3541 gtacaccgaa ggtagtgacc agcggaacat cattgatacc gaggaagccg tagcggaaac
     3601 cactgatcat ataaacgatt gggttcaggt gcgacagccc ttgccagaac ggcggcaaca
     3661 aagtcagtga gtaaaagacc ccgcccaaat acgtaagtgg cgttaacaca aaggttggca
     3721 ccaggctgat gtcatcgaac gttttggcaa acacgccgtt cagcaaaccc gcaagggaga
     3781 acaacaccgc cgtgagcacc agcgttaaag cgacaaatac ccacgaatgc acctgaaacg
     3841 gcacaaaaaa cagtgaaatt gccgtcacca gaatgccaac aaacagacca cgcgccacgc
     3901 caccgccgac atatccggca ataatgacgt gagtcggaac cggcgctacc agcagctctt
     3961 caatattacg ctgaaacttg gcaccaaaaa atgatgacgc gacgttagcg taggcgttgg
     4021 tgatgaccga catcatgatc agccccggta caatgaactg catgtagctg aagccatgca
     4081 tatcgccaat acgcgaacca atcaggttac cgaagataat aaagtaaagg gtcatggtga
     4141 tgaccggtgg caccagcgtc tgcacccaga tacgcataaa gcgatggatc tctttcgccc
     4201 agatgctttt tagtgccacc cagtaaagat gcatcatgcg cgatctcctt gtttttcatt
     4261 aaccagtgaa acaaacagct cttccagacg gttagctttg ttacgcatac ttaatacctg
     4321 aatgccctgc tcacttaact gcgtaaatac gctgttgatc ccctgctcac gcagaacttc
     4381 aacttccagc gtcgcggtat cgaccagtcg atactgatag ccatcgagct tcggtaacgg
     4441 gcttttcggt gcgagatcga gaataaaggt ttccgatttc agcttcgcca gcagcgcctt
     4501 catcgaggta ttttccacca gctcaccgtg ttgaataatg ccgatattgc ggcacagcat
     4561 ttctgcttct tccaggtagt gtgtggtgag aatgatagtg gtgcctttgt cgtttaaatc
     4621 cttcaaaaag ccccacattg agcggcgaag ttcgatatcc acgcctgcgg tcggttcgtc
     4681 gagaatcagt agtttaggtt catgcattaa cgcacgggca atcattaaac ggcgcttcat
     4741 cccgccagat aacatacgcg cacgttcgtt gcgttttccc catagatcga gttgtttaag
     4801 atacttttcg ctgcgaatgt acgcttcttt gcgctccaca ccgtagtacc ctgcctgatt
     4861 caccacaatt tgctgcacgg tttcaaacgg gttgaagtta aattcctgtg gcaccagacc
     4921 caactgacgt ttagcgttta cgacatcttt ctcgagatcg taaccaaata cgctgacccg
     4981 cccggaggtt ttatttacca gagagctgat aataccgata gtggtcgatt ttccggcccc
     5041 gttcggcccg agaagcgcat aaaaatcacc cgcttcgacc tgcaaatcga ttccacgaag
     5101 cgcctgaacg ccgcctggat aggttttttt aagctgttga agttccagtg caatggtcat
     5161 aaatttttac ttaccttacg ttcttacact ttatctgtgg tttaaatcgt cccggagttg
     5221 ccctatatta gccaaacgta attatttggt tacaggtcgt taacctccat gaaagacata
     5281 gatacactca tcagcaacaa tgcactatgg tcaaaaatgc tggtggaaga ggatcccggg
     5341 ttttttgaga aactggcaca agcgcaaaaa ccgcgctttc tatggattgg atgttccgac
     5401 agtcgcgttc ctgcagaacg tttaaccggt cttgagccgg gcgaactctt tgttcaccgt
     5461 aatgttgcta acctggtcat tcacaccgac ctgaactgcc tttccgtggt tcagtatgca
     5521 gtggatgtac tcgaagttga acacattatt atctgtggcc actacggttg cggcggcgta
     5581 caagccgcag ttgaaaaccc ggaactgggg cttatcaaca actggctgct gcatatccgc
     5641 gatatctggt tcaaacatag ctcattgctc ggcgaaatgc cgcaagagcg ccgtctggat
     5701 accttgtgtg aactgaacgt catggaacag gtgtataacc tgggccactc caccattatg
     5761 caatcagcgt ggaaacgcgg gcagaaagtt accattcacg gctgggccta cggcattcac
     5821 gacggcttgc tgcgtgatct ggatgtcaca gcgactaacc gcgaaaccct tgagcaacgt
     5881 taccgtcacg ggatctccaa cctcaagctg aaacacgcca accacaaata aaaatgccat
     5941 gccggatgca acacatccgg caacttcaca cttactcgtc cagcagaatc actttgccga
     6001 tatacggcag atgacggtaa cgctgtgcgt aatcaatgcc gtaacccacc acaaactcat
     6061 ccgggatcga gaaaccgata aattctaccg ggacgttcac ttcacgacgg gacggtttat
     6121 ccagcagcgt acaaatcgcc aacgacttcg gttcgcgcag gcttaagatc tcacgcactt
     6181 tcgacagtgt attccccgag tcgatgatat cttcaacaat cagcacgtcc ttgccacgga
     6241 tatcttcatc cagatctttg aggattttca catcacgggt ggtggacatg ccgctaccgt
     6301 agctggaggc ggtcataaaa tcgacttcat gagatacctg aacttcacgg cacaggtccg
     6361 ccataaacat aaatgagcca cgcagcagac caaccagcac catatcgctg ccactgtctt
     6421 tgtaacgctc agtaatctga cgacccagtt cggcgatacg cgctttaatc tccgcttcgg
     6481 ggatcattac ttctacagta tgtttcatat ctctaaccat atgatttaaa aacaaatcac
     6541 ttcgcacggg caggtttcag caccgcaaca aagcgcaaga cacgcagtat accagtaaaa
     6601 ctattcattc gcggaatctg ttaataaagc tcgaattgtg atgacgatca cacatgttaa
     6661 aacctataat taattgctac taaaatatta atgaattgaa atggtgtctc tttatggcaa
     6721 ttaacaatac cggctcgcgg cgattactcg tcacgctaac agcccttttt gcagcgcttt
     6781 gcgggctgta tctactcatt ggcggaggct ggctggtcgc gattggcggc tcctggtact
     6841 accctatcgc tggccttgtg atgctcggcg tcgcctggat gctgtggcgc agtaaacgcg
     6901 ccgcgctttg gctgtacgca gccctgctgc tcggcactat gatttggggc gtctgggaag
     6961 ttggtttcga cttctgggcg ctgactccgc gcagcgacat tctggtcttc ttcggcatct
     7021 ggctgatcct gccgtttgtc tggcgtcgcc tggtcattcc tgccagcggc gcagttgccg
     7081 cactggtggt ggcactgctg attagcggtg gtatcctggc ctgggccgga tttaacgatc
     7141 cgcaggaaat cagcggcacc ttaagcgccg ataccacgcc agcagaagcc atctcccccg
     7201 ttgccgatca ggactggcct gcctatggtc gcaatcagga aggtcaacgc ttttcacctc
     7261 tgaaacaaat taacgctgat aacgtccaca agctgaaaga agcctgggtg ttccgtactg
     7321 gcgatgtgaa gcagccgaac gatccaggtg aaatcaccaa tgaagtgacg ccaattaaag
     7381 tgggcgacac cctttacctg tgtaccgctc accagcgcct gtttgcgctc gatgccgcca
     7441 gcggcaaaga gaaatggcat tacgatcctg agctgaaaac caacgagtcc ttccagcatg
     7501 taacctgccg tggcgtctct tatcatgaag ccaaagcaga aactgcttcg ccggaagtga
     7561 tggcggattg cccgcgtcgt atcattcttc cggtcaatga tggtcgtctg attgcaatta
     7621 acgctgaaaa cggcaagctg tgcgaaacct tcgccaataa aggcgtgctc aatctgcaaa
     7681 gcaatatgcc ggacaccaaa ccgggtctgt atgaaccgac ttcgccgccg attatcaccg
     7741 ataaaaccat cgtgatggcc ggttcagtca ccgataactt ctcaacccgt gaaacatctg
     7801 gcgtgatccg tggttttgat gtcaacaccg gggagctgct gtgggctttt gatcctggtg
     7861 cgaaagatcc taacgcaatc ccgtctgacg aacacacctt tacctttaac tcgccaaact
     7921 cgtgggcacc agcggcctat gacgcgaagc tggatctggt gtatctgccg atgggcgtga
     7981 ccacgccgga tatctggggc ggtaaccgca caccggaaca ggaacgttat gccagctcga
     8041 ttctggcgct gaatgccaca accgggaaac tggcgtggag ctaccagacc gttcaccacg
     8101 acctgtggga tatggatctt ccggcacagc cgacgctggc ggatatcacc gttaatggtc
     8161 agaaagtgcc agttatttat gctccggcga aaaccggcaa catttttgtg ctcgatcgtc
     8221 gtaatggcga actggtggtt ccggcaccgg aaaaaccggt tccccaaggt gctgccaaag
     8281 gcgattacgt aaccccaact caaccgtttt ctgaactgag cttccgtccg acgaaaaatt
     8341 tgagcggtgc ggatatgtgg ggagccacca tgtttgacca gctggtgtgc cgcgtgatgt
     8401 tccaccagat gcgctatgaa ggcattttca ccccgccatc tgaacagggt acgctggtct
     8461 tccctggtaa cctggggatg ttcgaatggg gcgggatttc cgttgatccg aatcgtgaag
     8521 tggcaattgc caacccaatg gcactgccgt ttgtttcgaa actgatccca cgcggtcctg
     8581 gcaacccgat ggagcagccg aaagatgcca aaggcacggg tacggaatcc ggcattcagc
     8641 cgcagtacgg tgtaccgtat ggcgtcacgc tcaatccgtt cctctcaccg tttggtctgc
     8701 cgtgtaaaca gccagcatgg ggttatatct cggcgctgga tctgaaaacc aatgaagtgg
     8761 tgtggaagaa acgtattggt acgccgcagg acagtatgcc attcccgatg ccggtacctg
     8821 tgccgttcaa tatgggtatg ccgatgctgg gcgggccaat ctccacggcg ggtaacgtgc
     8881 tgtttatcgc cgctacggca gataactacc tgcgcgctta taacatgagc aacggtgaaa
     8941 aactgtggca aggtcgttta ccagcgggtg gtcaggcgac gccaatgacc tatgaagtga
     9001 atggtaagca gtatgtggtg atctccgcag gcggtcacgg ttcatttggt acgaagatgg
     9061 gcgactatat tgtggcttat gcgttgccgg atgatgtgaa gtaaggcttg ctcagattgc
     9121 tgacaacgtg cgatttgttc atgccggatg cggcgtgaac gccttatccg gcctacaaaa
     9181 catttgcaaa ttcaatacat tgcgcgtagg cctgataagc gtagcgcatc aggcagtttt
     9241 gcgtttgtca tcagtttcat gcccggagag atccgggcat atttccgaat acggtctttt
     9301 tataccgtaa accctaacat catccccgta tcttcatgct ccagcaaatg gcagtgcgcc
     9361 atataagcat gttctttcgg tgcatcgtga ttaaacttca ccagcacttc gctgacatta
     9421 ccttctacct taacggtatc tttccagcct gcgcgatgcg tcgctggcgg tttgccattt
     9481 tctgacaaga tacggaactg cgtaccgtgg atatggaacg gatgcagcat catgtcgccc
     9541 acgccagaga taacccaacg ttcgtattgc cctttcgccg ccgcaaacat cggcttgttc
     9601 atatcaaacg cctgaccgtt gattttattg gcatggtgga aatcgaactt cccgccgtgg
     9661 ttcatatgat tcatattgcc gtgccccata tggcccatca tctggctgtg atccatcccg
     9721 gccatcgcct gatcgccata tttctccatc agcatctgca tccccatcat atcgagcatt
     9781 gggtccatag aaagttgcag cttgcgtacc gtcagccctt ccagcgaagg taacgcaggc
     9841 aggctactta atgtgtctgg caaagcacca gaggcactaa tggcaatcgg ctgaatccgc
     9901 attaccggat gcggcttgtc aaacggcgca atcgccatcc ccatctggct gaccggcagc
     9961 gtcaccaggt caaagggttt gttgtcgtta acctccacca gcacttcaaa acgctcgccc
    10021 atcagcactg gcagctcgtt caccttcacc ggttcaggta gcagaccgcc gtcgctggca
    10081 atcacataaa gcgggcgatt gtcgctggtg gcgaaattga gcgagcgggc attacagcca
    10141 ttgagcaaac gcaggcgcag ccagccacgc ggggcagcgt gttgtgggta gattacaccg
    10201 ttggtcagca acgtatcgcc aaaccaaccc acagcggcgg tcatcacatc cagttgataa
    10261 tcaatctgcc cgtcagcgtt aaatttttta tcctgaacaa taaccggaac atcatcaata
    10321 ccccactgtt ttggcagcat taatttcagg atctcgtcat cttcaatcac caccaaacca
    10381 gccagcccca tcgccacctg acgtccggtc ttgccatgtt gatgtggatg gaaccagcag
    10441 gtagcggcag gttgatcaac gttcaacgtc accgagcgct tgccacctgg cggaataatt
    10501 ccctgcgggc cgccgtccac ttcacccggt acttccagcc cgtgccagtg caacgtcgtc
    10561 tcttccgtca gttggttgta gatatcaacc gttaccgctt tgccgcgctg taatttcacc
    10621 gccggcccca gcagattgcc gttatagccc caggtagttg cggttttccc gccaaaggtg
    10681 gactgacctg cgccaatagt taactgaatg cgattacggg catcggtcgt gagcaaatca
    10741 gggatcggta acgttgggcg ttccgccgca aatactgcgc ggctccacag cggcaaagcc
    10801 gaagccacgc ccagcgcgac ggaatatttt aagaaatcac gacgttgcat agttatttcc
    10861 ttattcttaa gcaggcgaaa caaaatcaaa cgttgagcat agtccttccc cttacgggaa
    10921 ggtcaagccg cagacacaat ttaatcgaca ataatggtcg tcgcctcgct cgcagtgtgc
    10981 taacgtttat cttctttaag cccctggtag aagcaatgaa gacgtttttc agaacagtgt
    11041 tattcggcag cctgatggcc gtctgcgcaa acagttacgc gctcagcgag tctgaagccg
    11101 aagatatggc cgatttaacg gcagtttttg tttttctgaa gaacgattgt ggttaccaga
    11161 acttacctaa cgggcaaatt cgtcgcgcac tggtcttttt tgcccagcaa aaccagtggg
    11221 atctcagtaa ttacgacacc ttcgacatga aagccctcgg tgaagacagc taccgcgatc
    11281 tcagcggcat tggcattccc gtcgctaaaa agtgcaaagc cctggcccgc gattccttaa
    11341 gcctgcttgc ctacgtcaaa taatccctga tacctttttg tagaaatatt gaccgtgcat
    11401 ccgcggtcaa tgttagctat tatgttgcgc ccttttttta cgggtgttaa caaaggaggt
    11461 atcaacccat ggccgaaaaa aaacagtggc atgaaacgct acacgaccag tttgggcagt
    11521 actttgcggt agataacgtt ctgtatcatg aaaagaccga tcaccaggat ctgatcattt
    11581 ttgagaacgc cgcatttggt cgcgtaatgg cgctggatgg cgtagtacaa accaccgagc
    11641 gcgacgagtt tatctatcat gagatgatga cccatgttcc gctactggcc cacggtcacg
    11701 cgaaacatgt gctgattatc ggcggcggcg acggtgccat gctgcgtgaa gtaacccgac
    11761 ataaaaacgt tgagtcaatc acgatggtgg aaatcgatgc gggtgtcgtg tcgttctgcc
    11821 gtcagtatct acccaaccat aacgccggta gctacgacga tccgcgcttt aaactggtga
    11881 tcgacgatgg cgtcaatttc gttaatcaaa ccagccagac ctttgatgtc attatttccg
    11941 actgcaccga tcctatcggt cccggcgaaa gtctgttcac ctcagcattt tatgaaggct
    12001 gcaaacgttg cctgaatcct ggcggtatct tcgtcgcaca aaacggcgtc tgctttttac
    12061 agcaggaaga ggccatcgac agccatcgca aactcagcca ttacttcagc gatgttggct
    12121 tttatcaggc ggcgatcccg acctattacg gcggcatcat gacctttgca tgggcgacag
    12181 ataacgacgc cttacgccat ctctcaaccg aaattattca ggcgcgtttt ctcgcttctg
    12241 gcctgaaatg ccgttattac aatccggcag tccatacggc agcttttgcc ttaccccagt
    12301 atctgcaaga tgcactggct tcacagccgt cctaaggaga agataagaaa ttgaaaaaac
    12361 tgaaactgca tggctttaat aatctgacca aaagtctgag tttttgtatt tacgatatct
    12421 gctacgccaa aactaccgaa gagcgcgacg gttatattgc ttatatcgat gaactctata
    12481 atgccaaccg tctgaccgaa atcctgtcag aaacctgttc cattatcggg gccaatattc
    12541 ttaacatcgc ccgccaggat tacgagccac aaggtgccag cgtcactatt ctggtgagtg
    12601 aagaaccggt cgacccgaaa cttatcgaca aaacagaaca ccccggccca ctgccagaaa
    12661 cggtcgttgc ccatctcgat aaaagtcata tttgcgtaca tacctacccg gaaagtcatc
    12721 ctgaaggcgg tttatgtacc ttccgcgccg atattgaagt ctctacctgc ggcgtgattt
    12781 ctccgctgaa ggcgctgaat tacctgatcc accagcttga gtccgatatt gtgactattg
    12841 attatcgcgt gcgcggtttt acccgcgaca ttaacggtat gaagcacttt atcgaccatg
    12901 agattaattc gattcagaac tttatgtctg acgatatgaa ggcgctgtat gacatggtgg
    12961 atgtaaacgt ctatcaggaa aatatcttcc ataccaagat gttgcttaaa gagttcgacc
    13021 ttaagcacta catgttccac accaaaccgg aagatttaac cgacagcgag cgccaggaaa
    13081 ttaccactgc gctgtggaaa gaaatgcgcg agatttatta cgggcgcaat atgcccgctg
    13141 tttaacgtct caggcggagc acggaggctc cgcctgattt acttacttct gctgcacgaa
    13201 attacggtaa gccgccacca cctgaagaaa atcctcaacg ccgcacagcg acaagctctc
    13261 ttcatcgtag tagttcatcc cctcttccat ttcatcgcca gcgaattcca gttgattggc
    13321 gcgaaccatc acctcttcac catccatcca tagcgtgtat tcatgccctg cccgttgcca
    13381 ggaacgttcg ctacctttca atgcgtgcgc cgcttgttct acttcatcaa gcaaggccag
    13441 gttttctttc acctcttcat taaaccagtg cccgaccact tcatgcccca tggacatgcg
    13501 cacctttacc actccggtaa tatcgcgcag aaattcgtaa tccatagtgt gttcctctgc
    13561 tcctggcaat gcgccgaaat cgcattgcgc cattgatgta attatcgcag ccttaccgga
    13621 gaaaaacagc ggaacggcga tggtttagaa ataaaaaatg cccgggaatc tcattcaccg
    13681 ggcattgtgt cgtttatgcg cagcgcgtgc gcttacttat taaaccgcag tctggaaaat
    13741 caccccgtcc gctttttcgg tgtactgaga aagctggtcg aagttcagat aacggtaagt
    13801 atcaacggct gttttatcta cctgcgccac gtaggtctgg tactcttccg gcgtcggcag
    13861 tttgccaatc agcgccgcaa cagcagccag ttccgcagaa gccaggaaga cattcgcgcc
    13921 agtacccaaa cggttcggga agttacgggt agaggtggaa accaccgtcg caccgtctgc
    13981 tacacgcgcc tggttaccca tacacaggga acagccaggg atctcgatac gagcaccact
    14041 cttaccgaag acgctgtagt agccttcttc ggtcagctgc gcagcgtcca tacgcgttgg
    14101 cggtgccacc cacaggcggg tcggcaactg acctttgtgc gcatccagca gtttaccagc
    14161 agcacggaag tgaccgatgt tagtcatgca agaaccgata aatacttcgt cgatcttctc
    14221 gccctgtacc gcagacagcg gacgtgcgtc gtccgggtca ttcggtgcac agaggattgg
    14281 ctctttgata tccgccagat cgatgtcgat cactgccgcg tattccgcat ctgcatcggc
    14341 ttccagcagc tcaggattcg ccagccattt ttccatgccc tgaatacgac gctccagggt
    14401 acgacgatcg ccgtaacctt ccgcgatcat ccacttcagc aggacgatgt tagagttcag
    14461 gtattcaatg atcggttctt tgttcagctt gatggtacaa ccggcagcag aacgctcggc
    14521 agacgcatcg gtcagctcaa atgcctgctc aactttcaga tccggcagac cttcaatttc
    14581 cagaatgcgg ccagagaaga tgtttttctt acctttcttc tcaacggtca gcagaccttg
    14641 tttgattgca tacagcggga tcgcgtgtac cagatcgcgc agggtgatac ccggctgcat
    14701 tttgcctttg aagcgcacca gaacggattc tggcatatcc agcggcatta cgcctgttgc
    14761 ggctgcaaat gccaccagac cagaacccgc cgggaaagag ataccgatcg ggaaacgggt
    14821 atgggagtca ccaccggtac cgacagtatc cggcagcagc atacggttca gccaggagtg
    14881 gattacgccg tcacccggac gcagcgacac accgccacgg ttcataatga agtccggcag
    14941 cgtgtggtgc gtgttaacgt caactggttt cggatacgcc gcggtgtggc agaaagactg
    15001 catcaccagg tcagccgaga agcccaggca cgccaggtct ttcagttcat cacgggtcat
    15061 cgggccggtg gtgtcctggg aacctacaga agtcattttc ggttcacagt acgcgcccgg
    15121 acgaatgcct ttcacgccac aggcacggcc taccattttc tgcgccagcg agaagccgcg
    15181 atcgctctca gcgacatctt tcgcctgacg gaacacatcg ctgtgcggca ggccaagtgc
    15241 ttcacgcgct ttggtggtca ggccacgccc aataatcagc ggaatacggc cgccagcacg
    15301 cacttcatca atcagcacgt cggttttcag ttcgaaggtc gccagcagtt cgccggtttc
    15361 gtggttacgc acttcacctt tgtacgggta aacgtcaatt acgtcgccca tgttcaggtt
    15421 agagacgtcg acttcgattg gcagcgcacc cgcgtcttcc atcgtgttaa agaagatggg
    15481 tgcaatttta ccgccgaggc acaaaccacc gccgcgtttg ttcggcacat gtggaatatc
    15541 atcgcccata aaccacagaa cggagttagt ggcggattta cgcgatgacc ccgtacccac
    15601 aacgtcaccg acgtacgcca gtgggaaacc tttctgttgc agagcttcga tttgcttaat
    15661 cggaccaaca acgcctggct gatctggctc aataccttca cgggcgtttt tcagcatcgc
    15721 cagcgcgtgc agtgggatat ccgggcgtga ccacgcatcc ggtgccggag agaggtcatc
    15781 ggtgttggtt tcgcctgtga ctttgaagac ggtaacggtc agtttttcag ccagcgccgg
    15841 gcgattcagg aaccattcgg catccgccca ggactgcatt acctgcttcg catattcgtt
    15901 gcctgctttc gctttctctt ctacgtcata gaagttatcg aacatcagca gcgtgtgaga
    15961 aagtgctttg gcagcgatcg gtgccagttt ggcatcatcc agcgcgtcga tcagcggatg
    16021 aatgttgtaa ccaccctgca tggtgcccag cagttcgatg gctttttccg gagtcagcag
    16081 aggggatttg gcttcgcctt tagcgatagc agccaggaag cctgctttta cataggcggc
    16141 ttcatcgacg cctgggggaa cacggttggt taacagatct aacaggaatt cttcttcgcc
    16201 cgcgggcggg tttttcagca gctctacaag cgcggccatt tggtttgcat ccaggggttt
    16261 gggcgcaatc ccctcagcgg cacgctcagc tacgtgctta cggtattctt ctagcacgac
    16321 ggttctcctc gctctcattg tcatagtgcg gcaggcgatt ctcttcacgc tcctgtgaga
    16381 cagcagtttg tagggtaaat gcccagtacc gcaacgggca gaatagcagg attttgacga
    16441 tgtgttaatc tgtttacaaa aaagcaacat aaaaaagtgg ctgaatcgtt aaggatggtc
    16501 tagagattgt ttccctccgt aaaaacttcg ttaattcgca tggtgataat cacctttctc
    16561 aacgaatccc aagattagtc aaaatttaaa ctaccgcctc tttatactcg gattcacagc
    16621 acctgcgggt ggcagttcgc cgaccattgc gatttccttg agatccgaat tatgaaaatg
    16681 actttgccgt ttaaacccca tgtgctggcg ctaatttgca gtgccgggct ttgtgccgcc
    16741 tctaccgggc tatatataaa aagccgcaca gtggaagcgc ctgtggaacc gcaatcgaca
    16801 caactggctg cgcctgacat ctccgcagtt acgcttcctg caacggtttc cgtgcctcct
    16861 gtaacacctg ccgtcgtcaa atccgcattc agcactgcgc aaatagatca atgggttgca
    16921 cctgtcgcgc tataccccga ctctctgctt tcacaagtgt taatggcatc aacctatccg
    16981 gcaaacgttg ctcaagcagt gcaatggtcg cacgataatc cacttaaaca aggcgatgct
    17041 gctattcagg cggtatctga ccagccctgg gacgccagcg tcaaatcact ggtggccttt
    17101 ccacagttga tggcattgat gggcgaaaac ccgcaatggg tgcaaaacct gggcgatgct
    17161 tttctggcgc agccgcagga ggtgatggac tcggtacagc gattgcggca actggcgcaa
    17221 caaaccggct cgctgaagtc atcaactgaa cagaaagtta ttaccacaac gaagaaagct
    17281 gtaccggtaa aacagacagt cacggctccc gtcataccat ccaataccgt ttcaactgcc
    17341 aaccctgtca ttacagagcc tgcaacaacc gtcatttcca ttgaacccac caatcctgat
    17401 gtggtctata ttcccaacta caacccaacc gtggtttacg ggaactgggc caatactgcg
    17461 tatccgccgg tttatctgcc gccgccagcc ggagaaccgt ttgttgacag ctttgtgcgc
    17521 ggattcggct atagcatggg cgttgctacc acgtacgcgc tattcagcag catcgactgg
    17581 gatgacgacg atcatgacca tcatcatcat gacgatgatg attatcacca tcacgatggc
    17641 ggtcatcgtg atggtaatgg ctggcaacac aatggcgaca acatcaatat cgacgtcaac
    17701 aatttcaacc gtatcaccgg tgaacatctt actgataaga atatggcatg gcggcacaat
    17761 ccaaactacc gtaatggtgt gccctatcat gatcaggata tggcaaagcg gtttcatcaa
    17821 accgatgtca acggcggaat gagcgccacg cagctacctg ctccaacacg cgacagccag
    17881 cgtcaggcgg cagcaagtca gtttcagcaa cgaacacaca gcgcaccagt cattacacga
    17941 gatacccaac gtcaggcagc ggcacagcgg tttaatgaag ctgaaaacta tgggagctat
    18001 gacgacttcc gcgacttcag ccgtcgccaa ccactgaccc agcaacaaaa ggacgccgct
    18061 cgtcagcgtt atcagtcggc ctctcctgag cagcgccagg cagtccgcga gaaaatgcag
    18121 actaacccgc agatccagca gcgaagagag gcagcgcgtg agcgcattca gtctgcaact
    18181 cccgaacagc gccaggtgtt taaggaaaaa gtacagcagc gcccactgaa tcaacagcaa
    18241 cgtgataacg cccgccagcg tgttcaatca gcatcacctg agcaacgtca ggtttttcgg
    18301 gagaaagttc aggagagccg cccgcaacgt ctaaacgaca gtgaccatac tgtcaggctg
    18361 aataacgagc aacggtcagc agtacgcgaa cgtctctctg agcgcggagc aaggcgactg
    18421 gaaaggtaaa ttactgacgt aaaaaaagcg gcgtggttag ccgctttttt aattgccaga
    18481 tgttccggca aacgaaaaat tacttcttct tcgctttcgg gttcggcagg tcggtaatgc
    18541 taccttcgaa cacttctgcc gccaggccca cagactcgtg cagagtcggg tgcgcatgga
    18601 tggtcagtgc gatgtcttca gcgtcacaac ccatttcgat tgccaggccg atttcaccca
    18661 gcaactcgcc gccgttagtg ccgacaatcg caccaccgat cacacggtga gattctttgt
    18721 cgaaaatcag cttggtcata ccgtctgcgc agtcggaagc gatagcacga ccagaagcag
    18781 cccacgggaa ggtggcggtt tcgtagctga tgcctttctc tttcgcttct ttctcagtca
    18841 gacctaccca tgcaacttct ggttcggtat aggcgatgga cgggataact ttcggatcga
    18901 agtaatgttt tttaccggcg ataacttcag cggcaacgtg accttcgtga acacctttgt
    18961 gtgccagcat tggctgaccg acgatatcac cgatagcaaa gatgtgcggt acgttggtac
    19021 gcagctgttt gtcaacgcgg atgaaaccac ggtcgtcaac ttccacgcca gctttgcctg
    19081 cgtcgaggtt tttaccgttc ggcacacgac caatcgctac cagcacggcg tcgtaacgct
    19141 gcggttcagc gggtgctttt ttgccttcca tcgtcacata aatgccgtct tctttcgctt
    19201 caacggcggt aactttggtt tccagcatca ggttgaattt cttgctgata cgcttggtaa
    19261 agactttaac gatgtcttta tcagctgccg ggatgacctg gtcgaacatt tcaaccacgt
    19321 caatctgtga acccagcgcg tggtatacgg tgcccatttc cagaccgatg ataccgccac
    19381 ccattaccag caggcgttct ggtacttctt tcagttccag cgcgtcagtg gagtcccaga
    19441 tacgcggatc ttcatgcgga ataaacggca gttgaatcgg gcgagaaccc gctgcaatga
    19501 tcgcgttgtc gaagttgatc acggttttac cgttctcacc ttcaacttcc agggtgttag
    19561 ccccggtaaa tttacccaga ccgttgacca ctttgacttt gcggcctttc gccataccag
    19621 ccagaccacc ggtcagctga ttgatcactt tctctttcca ggtacgaatc ttgtcgatat
    19681 cggttttcgg ttcgccaaag acgataccgt gttcagccag cgctttggct tcttcgataa
    19741 cttttgctac gtgcagcagt gctttagaag ggatacagcc gacgttcagg caaacaccgc
    19801 caagggtgtt gtagcgttct acgattacgg tttccagacc taaatcagcg caacggaagg
    19861 cagcggagta acctgcgggg cctgccccaa gtaccacgac ctgagttttg atttcagtac
    19921 tcatcatgac ctctatatat ttatctccgg cggtcatacc cgtcgtcttt caggccgcag
    19981 cggcgttagc ttcacgccct caccccagtc acttacttat gtaagctcct ggggattccg
    20041 gcgcttgcta ccttgctgca cccagaaatc catagggtat gtgaagtaat accctaacca
    20101 ccaccgggtc gttctatccg tcggtatttt acaaaattgt taacaatttt taaacaacaa
    20161 acggcaaccg atttgtctat tcgctaataa cctcaataca ttcatgagat taccagaaaa
    20221 aagccggccg ttgggccggc tcttttactt acatcaccag acggcgaatg tcagacagcg
    20281 tgttgttaat gatggtaatg aaacgggcac catcagcacc gtcgatcacg cggtggtcga
    20341 aggagagaga aatcggcagc atcagacgcg gcacgaactc tttaccattc cacaccggct
    20401 ccatcgcgga cttggaaacg ccgaggatag ccacttccgg cgcgttcaca atcggcgcga
    20461 agtgggtagt acccaggccg ccgatgctgg agatggtgaa gcaaccgccc tgcatttcgc
    20521 ccgcagtcag cttaccgtca cgcgctttct tagaaatagt catcagctcg cgagacagct
    20581 cgatgatgcc tttcttgttg acgtctttga ataccggaac aaccagaccg ttcggggtat
    20641 ccaccgccac accgatgttg atgtatttct tcagggtcag acgctgaccg tcttccgaca
    20701 gcgaactgtt gaagcgaggc atctgctcaa gagctgcagc aacggctttc atgatgaaga
    20761 caaccggggt gatcttcaca tccagcttac gtttcgccgc ttcttcgttc tgctgtttac
    20821 ggaacgcttc cagctcggtg atatcggttt tgtcgaagtg ggtaacatgc gggatcatca
    20881 cccagttacg gctcaggttc gcaccagaga ttttctggat acggcccagt tccacttctt
    20941 cgatttcacc aaacttgctg aagtccacct tcggccacgg cagcatgccc gggataccgc
    21001 cgccagtcgc cgccggagcc gcttctgcac gtttgatagc ttctttcacg taagcctgaa
    21061 cgtcttcgcg caggatacga cctttacggc cagtgccctt cactttcgcc aggttaacgc
    21121 caaattcgcg tgccagacgg cggatcagcg gagtcgcgtg aacgtaagcg tcgttttcag
    21181 caaattcaga tttgccttcg gctttcgcag ccggagctgc tgctggagct tcagcttttg
    21241 ctgccggagc cggcgctgcc gcttcctgtt tcgcaggagc tgccgcaggc gctgcgcctt
    21301 caacttcgaa gatcataatc agcgagccag ttttcacttt atcgccaacg ttgactttca
    21361 gttccttcac gacgcctgca aacggagccg gaacttccat agacgcttta tcgccttcta
    21421 cggtgatcag tgactgttca gcggcaactt tgtcgcccac tttcaccatc acttcggtca
    21481 cttcaacttc gtcaccgccg atatccggaa cgttaacgtc tttcacgcca gccgctggtg
    21541 cagaggccgc tgccggagcc gcttcctgtt tagccgccgg agctgccgcg cctgcttcac
    21601 ccgcgacttc gaagaccata atcagcgagc cggtagacac tttgtcaccc acgttcactt
    21661 tgatctcttt cacggtgcca gcaaacggag ccggaacttc catagaagct ttatcgcctt
    21721 ctacggtgat cagcgactgt tcagcttcaa ctttatcgcc aactttcacc aggatttcgg
    21781 tcacttcaac ttcgtcgctg ccgatatccg gaacgttaac gtcttttgcc gctgcagccg
    21841 ctggagctgc tgccggagcg gcttctttct tctcttctgc ctgagcaggt gcagcgtctg
    21901 ctgcaccgtc ggcggaatcg aaaatcataa tcagtgcgcc ggtctgggtt ttatcgccaa
    21961 cagagacttt gatctcttta acgatacccg cctgcggaga cggaacttcc atagaggctt
    22021 tgtcgccttc tacggtgatc agcgactgtt cggcttcaac tttgtcgccc actttgacca
    22081 ggatctcggt gatttcaact tcatcagccc cgatgtccgg tactttgatt tcgatagcca
    22141 ttattctttt acctcttacg ccagacgcgg gttaacttta tctgcatcga tgttgaattt
    22201 ggcgattgcg tcagcaacca ctttcttatc gatttcgcca cgtttagcca gttcgcccag
    22261 cgccgcaacc acgacataag aagcatcaac ctcgaagtgg tgacgcaggt tctcacggct
    22321 gtcggaacga ccgaagccat cagtacccag tacgcggtag tcgtcagccg gtacgtaagt
    22381 acggacttgc tcagcgaaca gtttcatata gtcggtagat gccactgccg gagcgtcgtt
    22441 catcacctga gcgatatacg gaacgcgcgg agtttccagc gggtgcagca tgttccagcg
    22501 ttcacaatcc tgaccatcac gcgccagttc ggtgaaggaa gtcacgctat aaacgtcaga
    22561 acctacgccg taatctttcg ccaggatctc tgctgcttca cggacgtgac gcaggataga
    22621 accggagccc agcagctgaa ctttaccttt gctaccttca atggtttcga gtttgtagat
    22681 acctttacgg ataccttcct cagcaccttc cggcattgct ggcatgtggt agttttcgtt
    22741 cagcgtagtg atgtagtagt aaacgttctc ttgtttctca ccgtacatac gctccagacc
    22801 gtcatgcata atgacagcaa cttcgtaagc gtaagccggg tcgtaagaga tacagttcgg
    22861 gatagtcagc gactgaatgt ggctgtgacc atcttcgtgc tgcagacctt cgccgttcag
    22921 ggtggtacga ccggaagtac cgccgatcag gaagccacgc gcttgctggt caccagccgc
    22981 ccagcacaga tcgccgatac gctggaagcc gaacatcgag taatagatgt agaacggaat
    23041 catcggcaga ttgttggtgc tgtaagaggt cgccgctgcc agccaggaac acccagcgcc
    23101 cagctcgttg atcccttcct gcagaatctg acctttctcg tcttctttat agtaagcaac
    23161 ctgctcgcgg tcctgcgggg tgtactgctg accgttcggg ctgtaaatac caatctgacg
    23221 gaacagacct tccataccga aagtacgcgc ttcgtcggcg atgatcggta ccagacgatc
    23281 tttgatcgac ttgttcttca gcattacgtt cagggcacga acgaaagcga tagtggtaga
    23341 gatctctttg ctctgctctt ccagcagcgc gccgaagtct tgcaggctcg gcagctcaag
    23401 cttctcggtg aagttcggct gacggcttgg cagataaccg tgcagtttct gacgctgagc
    23461 gtgcagatag gtatgctctt cagaaccttc cgggaaggtg atgtacggca atttttcgat
    23521 atcggcatca gacaccggca cattgaaacg gtcgcggatg tgacgcacgc cgtccatgtt
    23581 cattttctta acctggtgcg cgatgttttt accttcagcc gcgtcgccca tgccgtaacc
    23641 tttaatggta tgagcaagga ttaccgtcgc tttgcctttg gtttcctgcg ctttcttaaa
    23701 tgcagcgtag attttcttcg gatcgtgacc accacggttc agtgcccaga tctgttcgtc
    23761 agtccagtct gcaaccagtg ctgcggtttc aggatattta ccgaagaagt gttcacgaac
    23821 gtacgcacca tctttcgatt tgaaggtctg gtagtcgccg tcaacggttt cgttcatcag
    23881 ctggatcagt ttaccgctgg tatctttacg cagcagttca tcccaacggc taccccacat
    23941 cactttgatc acgttccagc cagcaccttc gaagatgcct tccagttcgt tgatgatctt
    24001 gccgttaccg gtgaccgggc cgtcaagacg ctgcaggtta cagttgataa cgaaaaccag
    24061 gttatccagt ttttcacggg tagcgatggt gatcgcacct ttggattccg gttcgtccat
    24121 ttcaccgtcg ccgaggaacg cgtaaacggt ttgtttagag gtatctttca ggccacggtg
    24181 ttccagatat ttcaggaatt tagcctggta aatagcacca atcggcccca gacccataga
    24241 tacggtcggg aactgccaga attccggcat cagtttcggg tgcggatagg aagagaggcc
    24301 attgccgtga acttcctgac ggaagttatc cagctgctcc tgagtcagac gaccttccag
    24361 gaaagcacga gcgtaaacgc ccggggagat gtggccctgg aagtaaacca ggtcgccgcc
    24421 atcctgctcg ttgcgtgcac ggaagaagtg gttaaagcac acatcataaa tggttgcgga
    24481 agactggaag gacgccatgt ggccgcccag ttcgaggtct tttttcgacg cacgcagaac
    24541 cgtcatgatg gcgttccagc ggatagctga acgaatacgg cgttccagtt ccagattacc
    24601 cggatactcc ggttgttctt caacggggat ggtgttgatg tagttgctga taccggtgcc
    24661 tgcggctacg ttgacaccgc ctttgcgggc ttcagcaagc agttggtcga tcagatactg
    24721 agcacgctca acaccttctt cacggatgac cgattcgatc gcctggagcc agtcgcgagt
    24781 ttcgatcgga tccacgtcat ttgggaaacg ttctgacatg ggttattcct tatctatcta
    24841 ataacgttga gttttctgga acctgtccca ttgaactctc gccggaaagc tcaataagac
    24901 aggttctacg tttagttgcc gcgcttttat atgcgcttga tttacaacat cttctggata
    24961 atttttacca gaaaaatcac taattctttc gttgctccag acgacgtaga gaacgctcac
    25021 ggcggctctc ttcacggctt ctgtcgagca aaatttcttc gataaaggcc agatggcgat
    25081 gcgatgcttc gcgcgcttct tccggcttac cggccataat cgcttcaaat atgcgggtgc
    25141 ggtgactact caccagcggc agcatctcgc gacgcgaata gagcaattcg aagttctggc
    25201 ggacattctg ggccaacatc ggctccatac accttagcag atgaagcaga accacattgt
    25261 gggccgcttc ggtgacggca atctgatact ggagtacggc atttgattcc gcgtccagat
    25321 cgccagactg ctgcgccagc tctatggcat ggtggagttc acggatgcgt tccttgtctt
    25381 catcggtact acgcagcgcg gcgtaataag cggcgatacc ttccagggcg tgtcgtgttt
    25441 cgagcaagtc atactgtgat tcaggatggt cggagagcag ctccaccagc ggatcgctga
    25501 agctttgcca taggctgctc tggacaaaag tgccgccacc ctggcgacga agcaacaagc
    25561 ccttcgcttc gagacgttga atcgcctcac gcaaggaggg acgggagacg tcgaactgtt
    25621 ttgccagttc gcgttccggt gggagttttt cgcccgggcg gagagtgcct tcgaggatca
    25681 aaaactccag ttgctgctca atcacatcgg agagttttgg ttggcggatt ttgctgtagg
    25741 ccatgagttc ctgtcttaag ccacttgccg aagtcaattg gtcttaccaa tttcatgtct
    25801 gtgacgctaa agtaacaaag tattcacctt atgtccatac aggttttgat tgaaatcatg
    25861 aaactgtgca cattttaaca acttgacata tataacgttt caaagttgta actatgcaca
    25921 aatgttaaat ttacccatca aggggtgaga ttaacagaat tgaaacgaaa aaatatgtcc
    25981 tctgaaccga tt
//
LOCUS       DAZFLD010000054        25769 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.054, whole genome shotgun sequence.
ACCESSION   DAZFLD010000054 DAZFLD010000000
VERSION     DAZFLD010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25769
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.054"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            122..1504
                     /gene="yicJ"
                     /locus_tag="ACHKBL_001865"
     CDS             122..1504
                     /gene="yicJ"
                     /locus_tag="ACHKBL_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide family
                     transporter"
                     /protein_id="HFO5818744.1"
                     /translation="MKSEVLSVKEKIGYGMGDAASHIIFDNVMLYMMFFYTDIFGIPA
                     GFVGTMFLVARALDAISDPCMGLLADRTRSRWGKFRPWVLFGALPFGIVCVLAYSTPD
                     LSMNGKMIYAAITYTLLTLLYTIVNIPYCALGGVITNDPTQRISLQSWRFVLATAGGM
                     LSTVLMMPLVNLIGGDNKPLGFQGGIAVLSVVAFMMLAFCFFTTKERVEAPPTTTSMR
                     EDLRDIWQNDQWRIVGLLTIFNILAVCVRGGAMMYYVTWILGTPEVFVAFLTTYCVGN
                     LIGSALAKPLTDWKCKVTIFWWTNALLAVISLAMFFVPMQASITMFVFIFVIGVLHQL
                     VTPIQWVMMSDTVDYGEWCNGKRLTGISFAGTLFVLKLGLAFGGALIGWMLAYGGYDA
                     AEKAQNSATISIIIALFTIVPAICYLLSAIIAKRYYSLTTHNLKTVMEQLAQGKRRCQ
                     QQFTSQEVQN"
     gene            1514..3832
                     /gene="yicI"
                     /locus_tag="ACHKBL_001866"
     CDS             1514..3832
                     /gene="yicI"
                     /locus_tag="ACHKBL_001866"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HFO5818745.1"
                     /translation="MKISDGNWLIQPGLNLIHPLQVFEVEQQGNEMVVYAAPRDVRER
                     TWQLDTPLFTLRFFSPQEGIVGVRIEHFQGALNNGPHYPLNILQDVKVTIENTERYAE
                     FKSGNLSARVSKGEFWSLDFLRNGERITGSQVKNNGYVQDTNNQRNYMFERLDLGVGE
                     TVYGLGERFTALVRNGQTVETWNRDGGTSTEQAYKNIPFYMTNRGYGVLVNHPQCVSF
                     EVGSEKVSKVQFSVESEYLEYFVIDGPTPKAVLDRYTRFTGRPALPPAWSFGLWLTTS
                     FTTNYDEATVNSFIDGMAERNLPLHVFHFDCFWMKAFQWCDFEWDPLTFPDPEGMIRR
                     LKAKGLKICVWINPYIGQKSPVFKELQEKGYLLKHPDGSLWQWDKWQPGLAIYDFTNP
                     DACKWYADKLKGLVAMGVDCFKTDFGERIPTDVQWFDGSDPQKMHNHYAYIYNELVWN
                     VLKDTVGEEEAVLFARSASVGAQKFPVHWGGDCYANYESMAESLRGGLSIGLSGFGFW
                     SHDIGGFENTAPAHVYKRWCAFGLLSSHSRLHGSKSYRVPWAYDDESCDVVRFFTQLK
                     CRMMPYLYREAARANARGTPMMRAMMMEFPDDPACDYLDRQYMLGDNVMVAPVFTEAG
                     DVQFYLPEGRWTHLWHNDELDGSRWHKQQHSFLSLPVYVRDNTLLALGNNEQRPDYAW
                     HEGTAFQLFNLQDGHEAVCEMPAADGSVIFTLKATRTGNTITVTGAGEAKNWTLCLRN
                     IVKVNGLQGGSQAESEQGLMVTPQGNALTITL"
     gene            4128..5681
                     /locus_tag="ACHKBL_001867"
     CDS             4128..5681
                     /locus_tag="ACHKBL_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFO5818746.1"
                     /translation="MQMITSRQNRLLRFLLPRREYTTIVTIAGYLNVSEKTIQRDLRL
                     LEQWLGQWRINVEKRAGAGVMLSAENITDLLHLDHLLVAECEEIDGVMNNSRRVKIAS
                     QLLSETPNETSISKLSERYFISGASIVNDLRVIESWLAPLGLSLIRSPSGTHIEGSEG
                     QVRQAMALLINGIINHNEPQGVVYSRLDPGSYKALVHYFGEEEVLFVQSLLLDMENEL
                     SWSLGEPYYVNIFTHILIMMYRNTHGNALSREEDQTRQYDENIFNVASQMIHKIEQRI
                     AHTLPDDEVWFIYQYIISSGVAIDGQKDVSIISHMQASNEARLITWRLITVFSDIVDC
                     DFSEDSALYDGLLVHIKPLINRLNYRIHIRNPLLEDIKAELADVWRLTQYVVNQVFKT
                     WGENAVSEDEVGYLTVHFQAAMERQIARKRVLLVCSTGIGTSHLLKSRILRAFPEWTI
                     VDVISAANLSQVLPDNIELIISTINLPTVTMPVAYVTAFFNDADIKRVTEMVITEKLH
                     HATSRVVEI"
     gene            5708..6178
                     /locus_tag="ACHKBL_001868"
     CDS             5708..6178
                     /locus_tag="ACHKBL_001868"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007370547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose PTS transporter subunit IIA"
                     /protein_id="HFO5818747.1"
                     /translation="MDITKILNTNRVILDMQATNKEEAIEELTNLLKKDGAIDCRETF
                     IKDVWQREAEGSTGFENHIAIPHGKSSAVINTTLAIGRTRQDIPWETLDGSSVRCIIL
                     FAVRLEDQNTTHIRLLSQVAGALADDDIIEQLLVETSPQKIIDLFSQYAQSDVC"
     gene            6196..6510
                     /locus_tag="ACHKBL_001869"
     CDS             6196..6510
                     /locus_tag="ACHKBL_001869"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001028073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_function: GO:0090563 - protein-phosphocysteine-sugar
                     phosphotransferase activity [Evidence IEA];
                     GO_function: GO:0090582 -
                     protein-phosphocysteine-D-fructose-phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HFO5818748.1"
                     /translation="MNIVCVAACTAGIAHTYIAREKLIKGAKALGHTIKVETQGTIGT
                     ENELQAADISAADVVILAVDVKIKGEERFTNKRIVRVKTEIVIKSPVQFLEKVEKSLG
                     NK"
     gene            6535..7626
                     /locus_tag="ACHKBL_001870"
     CDS             6535..7626
                     /locus_tag="ACHKBL_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFO5818749.1"
                     /translation="MKENKIPVSQEIKKHLLTGISWMIPLIVAAGICIALGQVLGGTD
                     VGEKTGTIPWMLNQIGGWGMGLIVPLISAAIAYSIADRPGFAPGLIVGFLCGQIHTGF
                     IGGMLGGFLVGYTILLLKRYIRLPQSMQGLMPIMVLPVLSTIIGGLLMMTLIGKPIAW
                     LQEALIHLLESMQGGSRFLMGAILGAMATFDFGGPVNKTMSLFSDGLLVSGVYGPEAV
                     KFVGSIIPPFGITLSFLLTRHKYTRAEREALKAAFPMGICMITEGVIPIAARDLLRVV
                     GSCVVASAVAGGLIMVWGVESPVPHGGMFVVPLFTHPLLFCLALGIGTVICGVMLSLW
                     KKPVTERDEEFDELSDQKLKDEEITFTLE"
     gene            7638..8489
                     /locus_tag="ACHKBL_001871"
     CDS             7638..8489
                     /locus_tag="ACHKBL_001871"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000457126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HFO5818750.1"
                     /translation="MFADMKSMVMKAWHEHYALLAINCMNLESAHAAIRVAEKNRAPI
                     ILNLYQGHLAHFPAPVAAAVVKTLAESASVPVALALDHGKNPDCIRQAFRAGFSGLMI
                     DASAFPLEENVRQTRAVVELAASVQLCVEGELGHLADAPRYDQAANADLMTQPTDVET
                     FIAQTGIDLLAVSVGTAHGMYAPGVVPAIDFQRLAEISRCSSVPLALHGGSGTPFDQL
                     QLCTTLGVAKINVGAAIFERGKSALLHTLCHDISIELVDALKAMELAFEEAITPYLQA
                     SGAIGKA"
     gene            8570..9430
                     /locus_tag="ACHKBL_001872"
     CDS             8570..9430
                     /locus_tag="ACHKBL_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFO5818751.1"
                     /translation="MLVSMKELLQPTRQHGFAIGAFNVADNCFLRAVVDEAEATNTPT
                     IIAIHPSEHDFVGDAFFAHVREITQRSPVPFVLHLDHGASVEHVLRAIRCGFTSVMID
                     GSLLPYEENVALTAEVVRLAHAVGVSVEGELGTIGQTGTSVEGGVSQVTYTDPAQAAD
                     FIARTGADTLAVAIGTAHGIYPKGMQPKLQMDILRDIAGRIDIPLVLHGGSANPDAEI
                     AESVTLGVGKINISSDMKYAYFQKVREILAKESWWDPNVIYPDAINAAREVIRHKMKL
                     FGSLGKASLY"
     gene            9478..10410
                     /locus_tag="ACHKBL_001873"
     CDS             9478..10410
                     /locus_tag="ACHKBL_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFO5818752.1"
                     /translation="MHYLGLDIGGTKIAAVVMDAHGWEIRRYRCPTQKSTYQQFVSCV
                     VALIEQIRRDVQRPMLTGIALPGSISPLTGLIKNANIQVINGHALQADLQQLLGQPVV
                     IANDGNCFALSEACDGAGQDYDVVFGITLGTGCGGGIAIKQRPFIGAWGNAAECGHIT
                     LPGYTEQEDGPSVSCYCGKHNCVESFVSGSGFSERYQQMTGNLLTPAAIVTLAQRGDA
                     CAMQQVARFRQQLARTLATIVNVVDPGVIVIGGGLSNVELLITDLNAEVAPLVFTDQF
                     TTPIVKAQHGDSSGMRGAAWLAMRNGEANETFTN"
     gene            10391..11071
                     /locus_tag="ACHKBL_001874"
     CDS             10391..11071
                     /locus_tag="ACHKBL_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lyxose/D-mannose family sugar isomerase"
                     /protein_id="HFO5818753.1"
                     /translation="MKRSQINYVIDKAHAIAQTFRVCLPEFAYFTVDDWLQRERDNWQ
                     EVVDLQLGWDITDFGRGDFNQTGLTLLTMRNGALGSAAYPKPYAEKMLQIQQDQQTPW
                     HFHTHKMEDILNRGGGDLCMQLAWANEANLCDDQRVITVSVDGQRRTMKPGETLVLKP
                     GQGICLPPRLYHRFWAEKAFVLGWEISMVNDDQHDNYFLEPGGRFPAIEEDEPVKWLL
                     CSEYGILR"
     gene            complement(11115..12824)
                     /gene="yicH"
                     /locus_tag="ACHKBL_001875"
     CDS             complement(11115..12824)
                     /gene="yicH"
                     /locus_tag="ACHKBL_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HFO5818754.1"
                     /translation="MKFIGKLLLYILIALLVVIAGLYFLLQTRWGAEHISAWVSENSD
                     YHLAFGAMDHRFSAPSHIVLENVTFGRDGQPATLVAKSVDIALSSRQLTEPRHVDTIL
                     LENGTLNLTDQTAPLPFKADRLQLRDMAFNSPNSEWKLSAQRVNGGVVPWSPEAGKVL
                     GTKAQIQFSAGSLSLNDVPATNVLIEGSIDNDRVTLTNLGADIARGTLTGNAQRNADG
                     SWQVENLRMADIRLQSEKSLTDFFAPLRSVPSLQIGRLEVIDARLQGPDWAVTDLDLS
                     LRNMTFSKDDWQTQEGKLSMNASEFIYGSLHLFDPIINAEFSPQGVALRQFTSRWEGG
                     MVRTSGSWLRDGKTLLLDDAAIAGLEYTLPKNWQQLWMETTPGWLNSLQLKRFSASRN
                     LIIDIDPDFPWQLTALDGYGANLTLVTDHKWGVWSGSANLNAAAATFNRVDVRRPSLA
                     LTANSSTVNISELSAFTEKGILEATASVSQTPQRQTHISLNGRGVPVNILQQWGWPEL
                     PLTGDGNIQLTASGDIQANVPLKPTVTGQLHAVNAAKQQVTQTMNAGVVSSGEVTSTE
                     PVQ"
     gene            complement(12945..14336)
                     /gene="xanP"
                     /locus_tag="ACHKBL_001876"
     CDS             complement(12945..14336)
                     /gene="xanP"
                     /locus_tag="ACHKBL_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanP"
                     /protein_id="HFO5818755.1"
                     /translation="MSVSTLESENAQPVAQTQNSELIYRLEDRPPLPQTLFAACQHLL
                     AMFVAVITPALLICQALGLPAQDTQHIISMSLFASGVASIIQIKAWGPVGSGLLSIQG
                     TSFNFVAPLIMGGTALKTGGADVPTMMAALFGTLMLASCTEMVISRVLHLARRIITPL
                     VSGVVVMIIGLSLIQVGLTSIGGGYAAMSDNTFGAPKNLLLAGVVLALIILLNRQRNP
                     YLRVASLVIAMAAGYALAWFMGMLPESNEPMTQELIMVPTPLYYGLGIEWSLLLPLML
                     VFMITSLETIGDITATSDVSEQPVSGPLYMKRLKGGVLANGLNSFVSAVFNTFPNSCF
                     GQNNGVIQLTGVASRYVGFVVALMLIVLGLFPAVSGFVQHIPEPVLGGATLVMFGTIA
                     ASGVRIVSREPLNRRAILIIALSLAVGLGVSQQPLILQFAPEWLKNLLSSGIAAGGIT
                     AIVLNLIFPPEKQ"
     gene            14615..15820
                     /gene="gltS"
                     /locus_tag="ACHKBL_001877"
     CDS             14615..15820
                     /gene="gltS"
                     /locus_tag="ACHKBL_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015501 - glutamate:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015813 - L-glutamate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HFO5818756.1"
                     /translation="MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLL
                     VALALLVLKKSMGWEVNFDMSLRDPLMLAFFATIGLNANIASLRAGGRVVGIFLIVVV
                     GLLVMQNAIGIGMASLLGLDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEV
                     AMACATFGLVLGGLIGGPVARYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLI
                     ETIALIAICLTVGKIVAQLLAGTAFELPTFVCVLFVGVILSNGLSMMGFYRVFERAVS
                     VLGNVSLSLFLAMALMGLKLWELASLALPMLAILVVQTIFMALYAIFVTWRMMGKNYD
                     AAVLAAGHCGFGLGATPTAIANMQAITERFGPSHMAFLVVPMVGAFFIDIVNALVIKL
                     YLMLPIFAG"
     gene            complement(15854..17935)
                     /gene="recG"
                     /locus_tag="ACHKBL_001878"
     CDS             complement(15854..17935)
                     /gene="recG"
                     /locus_tag="ACHKBL_001878"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HFO5818757.1"
                     /translation="MKGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRY
                     EDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRMMTCQISDGSGILTMRFFNFNAA
                     MKNSLATGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
                     ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQH
                     PAQRRLILEELLAHNLSMLALRAGAQRFHAQPLSANDALKNKLLAALPFKPTGAQARV
                     VAEIERDMALDAPMMRLVQGDVGSGKTLVAALAALRAIAHGKQVALMAPTELLAEQHA
                     NNFRNWFAPLGIEVGWLAGKQKGKARLAQQEAIASGQVQMIVGTHAIFQEQVQFNGLA
                     LVIIDEQHRFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDE
                     LPPGRTPVTTVAIPDTRRTDIIDRVRHACITEGRQAYWVCTLIEESELLEAQAAEATW
                     EELKLALPELNVGLVHGRMKPAEKQAVMASFKQGELHLLVATTVIEVGVDVPNASLMI
                     IENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQ
                     KDLEIRGPGELLGTRQTGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIER
                     WMPETERYSNA"
     gene            complement(17941..18630)
                     /gene="trmH"
                     /locus_tag="ACHKBL_001879"
     CDS             complement(17941..18630)
                     /gene="trmH"
                     /locus_tag="ACHKBL_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0141100 - tRNA
                     (guanine(18)-2'-O)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TrmH"
                     /protein_id="HFO5818758.1"
                     /translation="MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVG
                     VHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNA
                     VDFREIDYTRPTCILMGQEKTGITQEALALADRDIIIPMIGMVQSLNVSVASALILYE
                     AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEAD
                     ADWWATMQAAG"
     gene            complement(18637..20745)
                     /gene="spoT"
                     /locus_tag="ACHKBL_001880"
     CDS             complement(18637..20745)
                     /gene="spoT"
                     /locus_tag="ACHKBL_001880"
                     /EC_number="2.7.6.5"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008728 - GTP diphosphokinase activity
                     [Evidence IEA];
                     GO_function: GO:0008893 - guanosine-3',5'-bis(diphosphate)
                     3'-diphosphatase activity [Evidence IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HFO5818759.1"
                     /translation="MYLFESLNQLIQTYLPEDQIKRLRQAYLVARDAHEGQTRSSGEP
                     YITHPVAVACILAEMKLDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSK
                     LDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
                     ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILS
                     EIEGRLQEAGIPCRVSGREKHLYSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRV
                     LGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMG
                     VAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFT
                     PEGRIVELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLTSGQTVEIITAPG
                     ARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLLNHALGGSRKLNEIPQENI
                     QRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPATQSHGHLPIKGADGV
                     LITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQE
                     FITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSAFIRLTARDRVHLA
                     NIMRKIRVMPDVIKVTRNRN"
     gene            complement(20764..21039)
                     /gene="rpoZ"
                     /locus_tag="ACHKBL_001881"
     CDS             complement(20764..21039)
                     /gene="rpoZ"
                     /locus_tag="ACHKBL_001881"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013511051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFO5818760.1"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEEND
                     KTTVIALREIEEGLINNQILDVRERQEQQEQEAAELQAVTAIAEGRR"
     gene            complement(21094..21717)
                     /gene="gmk"
                     /locus_tag="ACHKBL_001882"
     CDS             complement(21094..21717)
                     /gene="gmk"
                     /locus_tag="ACHKBL_001882"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFO5818761.1"
                     /translation="MAQGTLYIVSAPSGAGKSSLIQALLKTQPLYDTQVSVSHTTRQP
                     RPGEVHGEHYFFVNHDEFKEMISRDAFLEHAEVFGNYYGTSREAIEQVLATGVDVFLD
                     IDWQGAQQIRQKMPHARSIFILPPSKIELDRRLRGRGQDSEEVIAKRMAQAVAEMSHY
                     AEYDYLIVNDDFDTALTDLKTIIRAERLRMSRQKQRHDALISKLLAD"
     gene            21975..23660
                     /gene="ligB"
                     /locus_tag="ACHKBL_001883"
     CDS             21975..23660
                     /gene="ligB"
                     /locus_tag="ACHKBL_001883"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418104.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigB"
                     /protein_id="HFO5818762.1"
                     /translation="MKVWMAILISILCWQSSAWAVCPAWSPARAQEEISRLQQQIKQW
                     DDDYWKEGKSEVEDGIYDQLSARLTQWQRCFGEETHRDAMMPPLNGAVIHPVAHTGVR
                     KMADKIALSLWMRERSDLWVQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQKVR
                     LISAVPQTVSGPLANSTLQGEIFLKREGHIQKQMGGINARAKVAGLMMRQDDSDTLNS
                     LGVFVWAWPDGPQLMTDRLKELATAGFTLTQTYTRAVKNADEVARVRNAWWKTKLPFV
                     TDGVVVRAAKEPESRHWLPGQAEWLVAWKYQPVARVAEVKAIQFAVGKSGKISVVASL
                     APVMLDDKKVQRVNIGSVRRWQEWDIAPGDQILVSLAGQGIPRIDDVVWRGAERTKPT
                     PPENRFNSLTCYFASDVCQEQFISRLVWLGSKQVLGLDGIGEAGWRSLHQTHRFEHIF
                     SWLLLTPEQLQNTPGIAKSKSAQLWHQFNLARKQPFTRWVMAMGIPLTRAALNASDER
                     SWSQLLLSTEQFWQQLPGTGSGRVRQVIEWKENAQIKKLGSWLAAQQITGFEP"
     gene            complement(23657..24274)
                     /gene="yicG"
                     /locus_tag="ACHKBL_001884"
     CDS             complement(23657..24274)
                     /gene="yicG"
                     /locus_tag="ACHKBL_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HFO5818763.1"
                     /translation="MLLHILYLVGITAEAMTGALAAGRRRMDTFGVIIIATATAIGGG
                     SVRDILLGHYPLGWVKHPEYVIIVATAAVLTTIVAPVMPYLRKVFLVLDALGLVVFSI
                     IGAQVALDMGHGPIIAVVAAVTTGVFGGVLRDMFCKRIPLVFQKELYAGVSFASAVLY
                     IALQHYVSNHDVVIISTLVFGFFARLLALRLKLGLPVFYYSHEGH"
     gene            complement(24566..25390)
                     /gene="dinD"
                     /locus_tag="ACHKBL_001885"
     CDS             complement(24566..25390)
                     /gene="dinD"
                     /locus_tag="ACHKBL_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HFO5818764.1"
                     /translation="MNEHHQPFEEIKLINANGAEQWSARQLGKLLGYSEYRHFIPVLT
                     RAKEACLNSGHTIDDHFEEILDMVKIGSNAKRALKDIVLSRYACYLVVQNGDPAKPVI
                     AAGQTYFAIQTRRQELADDEAFRQLREDEKRLFLRNELKEHNKQLVEAAQQAGVATAI
                     DFAIFQNHGYRGLYGGLDQKAIHQRKGLKKSQKILDHMGSTELAANLFRATQTEEKLK
                     RDGVNSKQQANTTHFDVGRKVRQTIQELGGTMPEELPTPQVNIKQLENSVKITEKK"
BASE COUNT         6066 a         6257 c         6917 g         6529 t
ORIGIN      
        1 acgatcacat aaaacaagaa tcataaatta ataaccagat atcggaatat tcgctctcgc
       61 agggatggtt tacaaaatgc gttatcggtc tcagcacccc tatagcatca aggaaaagca
      121 gatgaagagt gaagtgttgt ccgttaaaga gaaaattggt tatggcatgg gagacgccgc
      181 cagccacatt attttcgata acgtaatgtt atatatgatg ttcttttata ccgatatttt
      241 tggcattcct gccggatttg tcggaaccat gtttttggtc gctcgtgcac tggatgcgat
      301 ttccgatcct tgcatggggt tgttggccga tcgaacgcgc tctcgctggg gtaaatttcg
      361 tccgtgggta ctgtttggcg cactgccatt tgggatcgtc tgtgtactgg cctatagcac
      421 gccagatctc agtatgaacg gaaaaatgat ttatgcagca attacttaca ccctacttac
      481 cttactttat accatcgtta atatccctta ctgcgcattg ggtggtgtaa tcaccaatga
      541 cccgactcag cgtatctcgc tgcaatcctg gcgttttgtg ctggcgaccg cgggaggcat
      601 gctctctact gtgctgatga tgccgctggt taatttaatt ggtggtgata ataaaccact
      661 cggtttccag ggcggtatcg cggtcctttc cgtggtggca ttcatgatgc tggcattctg
      721 tttcttcacc actaaagaac gcgttgaagc accacctaca acgacgtcta tgcgggaaga
      781 tttacgtgat atctggcaaa acgaccagtg gcggattgtc ggtttactaa ccattttcaa
      841 tattctggcg gtgtgcgtac gcggtggagc gatgatgtat tacgtcacat ggattttggg
      901 tacgccggaa gtgtttgtcg cttttctcac cacttattgc gtgggtaacc tgattggttc
      961 cgcactggca aaaccgctga ccgactggaa atgtaaagtc actatcttct ggtggacgaa
     1021 cgccctgctg gcagtgatta gcctcgcgat gttctttgtt cccatgcagg ccagcatcac
     1081 tatgtttgtc ttcatcttcg tgattggtgt attgcatcaa ctggtgacac ctatccagtg
     1141 ggtaatgatg tccgataccg tcgactacgg cgagtggtgc aatggtaaac gcctgaccgg
     1201 gatcagtttt gctggcacgc tgtttgtgct caagctgggg ttggccttcg gcggcgctct
     1261 tatcggctgg atgctggctt atggcggata tgatgcggca gaaaaagcgc agaacagcgc
     1321 cacgattagc atcattatcg cgctattcac gattgttccg gcgatctgtt acttgctgag
     1381 cgcgattatc gctaaacgct actactcact cacgacgcac aatctgaaaa ccgttatgga
     1441 acagctggcg cagggcaaac gccgttgcca gcaacaattc acctctcaag aagtgcagaa
     1501 ctaaggaacg gcaatgaaaa ttagcgacgg aaactggttg attcaacctg gcctcaattt
     1561 gattcacccg cttcaggtgt tcgaggttga acagcagggt aatgaaatgg tggtctatgc
     1621 tgccccccgt gatgtgcgtg aacgtacctg gcagcttgat acacctttat ttacgctgcg
     1681 ctttttctcc ccacaggaag gtattgttgg tgtacggatt gagcattttc agggggcgct
     1741 gaataacggt cctcattatc cgctcaatat tttgcaggat gtgaaggtca caatcgaaaa
     1801 cacagaacgt tatgctgagt ttaaaagtgg caacttaagc gcgcgtgtca gcaaaggtga
     1861 gttctggtca ctggattttc tgcgcaacgg cgaacgtatt accggtagtc aggtgaaaaa
     1921 taatggctac gtgcaggaca cgaataatca acgaaattat atgtttgagc ggctggatct
     1981 tggcgttggc gaaacagttt acggtctggg agagcgcttt actgccctgg tgcgcaatgg
     2041 ccagacggta gagacctgga accgggacgg cggcacaagt actgaacagg cgtataaaaa
     2101 tatcccgttc tatatgacta accgtggtta tggggtactg gtcaatcatc ctcaatgcgt
     2161 ctcttttgaa gtgggatcgg agaaagtctc caaagtgcag ttcagcgttg agagtgaata
     2221 tctcgaatac tttgttatcg acggcccgac gccgaaagcg gtacttgatc gttatacccg
     2281 ttttactggt cgtccggcgc tgccgcccgc gtggtccttc ggcctgtggc taaccacttc
     2341 atttaccacc aactacgacg aagcgacggt aaacagcttt atcgatggta tggcggaacg
     2401 caatctgccg ctgcatgttt tccactttga ctgtttctgg atgaaagcct tccagtggtg
     2461 cgattttgag tgggacccgc tgactttccc ggacccggaa gggatgatcc gccgcctgaa
     2521 agcgaaaggg ctaaaaatct gcgtctggat taacccctat attgggcaaa aatcccctgt
     2581 ctttaaagag ttacaagaga aaggctattt actcaaacac ccggacggtt cgttatggca
     2641 gtgggataaa tggcagccag gtctggcaat ttatgacttt accaatccgg atgcctgcaa
     2701 atggtacgcc gacaaactga aaggtctggt cgcgatgggc gttgattgct ttaagaccga
     2761 ctttggcgaa cgtattccaa ccgatgttca atggtttgac ggttccgatc cgcagaaaat
     2821 gcataaccat tatgcgtaca tctacaacga actggtgtgg aacgtgctca aggacaccgt
     2881 tggtgaggaa gaagctgtct tgtttgcccg ctcggcttcc gtcggtgcgc agaaattccc
     2941 ggtacactgg ggtggcgact gttacgctaa ctacgaatca atggcggaaa gcctgcgcgg
     3001 tggtttgtct attggccttt caggttttgg cttctggagc cacgatatcg gcggctttga
     3061 gaataccgcg ccggcgcatg tttacaaacg ctggtgcgcg tttggtttgc tctccagcca
     3121 tagccgttta catggcagca aatcttatcg tgtgccgtgg gcctacgatg atgagtcctg
     3181 tgatgtggtg cgcttcttca cgcaactgaa atgccgcatg atgccgtatc tgtatcgtga
     3241 agctgctcgt gcgaacgcgc ggggtacgcc gatgatgcgg gccatgatga tggagtttcc
     3301 ggacgatccg gcttgtgatt accttgaccg tcaatacatg ttaggcgaca acgtgatggt
     3361 tgcgcctgtg ttcactgaag cgggcgatgt gcagttctac ttgcctgaag gtcgctggac
     3421 acacctgtgg cacaacgatg aactcgatgg tagtcgctgg cataaacagc agcacagctt
     3481 cctgagtctg cccgtttatg tgcgtgataa caccctactg gcgctgggca acaacgagca
     3541 acgtcccgat tacgcgtggc acgaaggcac ggcattccag ctctttaatc tacaagacgg
     3601 gcatgaagcc gtctgtgaaa tgcccgctgc tgacggttcg gtgatcttta ctctaaaagc
     3661 aacacgtact ggtaacacga ttactgtgac tggtgcgggc gaggcgaaga actggacact
     3721 gtgcttgcgc aatattgtga aagtaaatgg tctgcaaggc ggttcgcagg ctgaaagtga
     3781 gcaggggctg atggtgacgc ctcaagggaa tgcgctgaca attacgttgt aatctattga
     3841 tggaatgccg ggtacgaatg gttttgtgtc gtatccggta aataatctca catctggtaa
     3901 taaatatttc ttccatcgta tttgcgagca tgatcacgat tattcatcgc gaaaatgtga
     3961 tctgtgctga aaaatatccc ttaaccgcct tgctttgttt tatcactgtc cggattacgg
     4021 acagatttta gtattgttgt gaaattattc tgctttatat ttaccctcag agatggcaac
     4081 tgaagctctt cctcatgagg cttcttaatc tgtgataagg gtaaaaaatg caaatgatta
     4141 cctcgcgaca aaacagatta ttacgattcc ttttacctcg aagggaatat acgactattg
     4201 ttacgattgc cggctattta aatgtttcgg aaaaaaccat tcaacgtgat ttacgtttac
     4261 ttgagcaatg gctgggacaa tggagaataa atgttgagaa gcgtgctggc gcgggtgtga
     4321 tgttaagcgc ggagaatatt actgatttgc tgcatcttga tcatttgttg gtggcagaat
     4381 gtgaagagat tgatggtgta atgaataatt ccaggcgcgt taaaatagcg tcgcagttat
     4441 taagtgaaac accgaatgaa acgtcgatca gtaaattgtc agaacgctac tttatcagcg
     4501 gagcctctat tgttaatgac ctgagagtaa ttgagtcctg gcttgcgccg ttggggttat
     4561 cgttgatccg cagtccaagt ggtacgcata ttgaaggtag tgaagggcaa gtccgacagg
     4621 caatggcatt actgattaac ggcattatta accataatga gccgcaaggt gtcgtgtatt
     4681 cacgtctgga tcccggaagc tataaagcat tagtccatta ttttggagaa gaagaggtgt
     4741 tatttgtcca gtcattgtta ctggatatgg aaaatgaatt aagttggtct ttgggagaac
     4801 cttattacgt taacattttt actcacatcc ttattatgat gtatcgcaac acgcacggga
     4861 atgcgttatc aagagaagaa gatcaaacca ggcaatatga tgaaaatatc tttaatgttg
     4921 ccagtcagat gattcataag atagaacaac gaattgcaca tacattgccc gatgatgaag
     4981 tctggtttat ttatcaatat atcatttcat caggtgtggc gattgatgga caaaaagatg
     5041 ttagcattat ttcacatatg caggccagca atgaagcgcg tctgattacc tggcgtttaa
     5101 ttacggtatt cagtgacatc gtggactgcg attttagtga agacagcgca ttatatgatg
     5161 gcttattggt gcatattaaa ccgctgatta accgactaaa ttatcgtatt catatccgta
     5221 atccattgtt ggaagatatt aaagcagaac tagcggatgt ctggcggttg acgcaatatg
     5281 tggtgaatca ggtatttaaa acctggggtg agaatgcagt gagcgaggat gaagtaggtt
     5341 acctgaccgt tcattttcag gctgcgatgg agcggcaaat tgcccgtaaa cgcgtattac
     5401 tggtctgttc aaccggaatc ggaacttcgc atctactgaa aagccgtatt ctgcgagcat
     5461 ttcctgaatg gacgattgtt gatgttattt cagcagcgaa tttatcacag gttttgcctg
     5521 acaatatcga actgattatt tcgaccatta atttgcctac agtcactatg ccggtcgctt
     5581 atgttaccgc tttttttaat gatgccgata ttaagcgggt cactgaaatg gtgattacgg
     5641 aaaaattaca tcatgcgaca tctcgggtcg ttgaaattta atgtcagaat tttatgaggt
     5701 atgacgaatg gacataacaa aaatcctgaa tacgaatcgc gttattttag atatgcaagc
     5761 gacgaataaa gaggaggcga ttgaagaatt aactaatctt ctcaaaaaag atggtgcgat
     5821 cgattgtcgg gaaaccttta ttaaagatgt ctggcagcgc gaagcggaag gctcgactgg
     5881 ttttgaaaat catattgcga tcccgcatgg taaatcttca gctgtgatta atactacgct
     5941 ggcaattggc cgtacccggc aggatattcc ctgggaaacg ctggatggca gtagcgtgcg
     6001 ctgtattatt ttatttgcgg tacgtcttga agatcaaaat accacccata tccgtttact
     6061 ttcccaggtc gcgggcgcgc tggcggatga tgacatcata gaacaattgt tagttgagac
     6121 gagtccgcaa aaaatcattg atctctttag tcagtatgca caatccgatg tctgctaaat
     6181 aacagggagt atgtcatgaa tattgtctgt gttgccgcct gtacggcggg tattgctcat
     6241 acctatattg ctcgtgaaaa gttaatcaaa ggagcaaaag ctctgggtca taccattaaa
     6301 gttgaaaccc agggaaccat tggtacagaa aatgaattac aggcggcaga tatctctgct
     6361 gctgacgtcg tgattcttgc tgtagatgtg aaaataaaag gagaagaacg cttcaccaat
     6421 aaacgtattg tgcgagtaaa gacagagatc gtcattaaat cgcccgttca atttcttgaa
     6481 aaggtcgaaa aatcactggg aaacaaatga tccttatact ggagggatct taatatgaaa
     6541 gaaaataaaa ttccggtttc acaggaaatt aaaaaacatt tattaaccgg catctcgtgg
     6601 atgatacccc ttattgtggc cgccgggata tgtattgcgt tggggcaagt tcttggtggt
     6661 acagatgttg gtgaaaaaac aggaacaatc ccgtggatgc ttaatcagat tggcggctgg
     6721 ggaatggggc tgattgtgcc gctgattagc gcggcaattg cctattctat tgccgatcgt
     6781 cccggatttg ccccgggcct gattgtcggc tttctctgtg ggcaaatcca taccggattt
     6841 attggcggta tgttgggtgg tttcctggtg ggttacacca ttttgctact taaacgctat
     6901 atccgcctgc cgcagtcgat gcaaggactc atgccaatca tggtgttgcc ggtattaagc
     6961 accattatcg gtgggctgtt aatgatgacg ctgattggta aacccatcgc ctggttacaa
     7021 gaagcactga ttcatttgct cgagtcgatg cagggcggat cgcgcttcct gatgggggcc
     7081 atacttggcg cgatggcaac cttcgatttc ggtggtccgg tcaacaaaac catgtcgctg
     7141 ttttccgatg gcttgctggt cagtggcgtc tacggaccag aagccgtgaa atttgtcggc
     7201 tcaatcatcc ctccgtttgg catcacactt tcgttcttac tgacacgcca taaatacacc
     7261 cgtgcggagc gcgaggcgct gaaagccgcg tttccgatgg ggatctgcat gatcactgaa
     7321 ggagtcattc cgatcgccgc gcgtgacctg ctgcgagtag tgggatcttg tgtggtggca
     7381 tcggcggtcg cgggcggctt aatcatggtg tggggcgtgg agagtccggt tccgcatggc
     7441 ggcatgtttg ttgtgcctct gttcacccat cctctgctgt tttgcctggc gctgggtata
     7501 ggcacggtga tttgtggcgt gatgctttca ctctggaaga aaccggtaac ggaacgcgac
     7561 gaagagtttg acgaactgag cgatcaaaag ctgaaagatg aagagatcac tttcaccctc
     7621 gaataaccgg aggccctatg tttgccgata tgaaaagtat ggtgatgaaa gcctggcatg
     7681 aacattacgc tttgctggcg atcaactgta tgaatctgga aagtgctcac gcagctatcc
     7741 gcgtggcgga gaagaatcgc gctccgatca ttctcaatct ctatcagggg catctggcgc
     7801 attttcctgc acccgtcgcg gcggcagtgg ttaaaacgct cgcggaatct gcctcagtac
     7861 cggtcgctct ggcgcttgat catggcaaaa atccggactg tattcgtcag gcgtttcgtg
     7921 ctggtttcag cggattaatg attgatgctt ctgcttttcc cctggaggaa aacgtgcgcc
     7981 agaccagggc ggtggtggaa ctcgccgcca gcgtgcagct ctgcgtggaa ggcgaactgg
     8041 gccatctggc agatgcgccg cgttacgacc aggccgccaa tgcagacctg atgacgcaac
     8101 ccactgatgt ggaaacgttt atcgcgcaga caggcattga tctactggcc gtctcggtag
     8161 gcacggcgca cggcatgtac gcgcctggtg ttgtacctgc tattgatttt cagcgtctgg
     8221 cggagatttc gcgctgctcg agcgtgcctc tggcattaca tggcggtagc ggtacgccgt
     8281 tcgatcagct ccagctctgt acgacgctgg gggtagcaaa aatcaacgtg ggggcggcca
     8341 tttttgaacg ggggaaatcc gcgctcctgc ataccctctg tcacgatata tccattgaac
     8401 tggtggacgc gctgaaagct atggaactgg ccttcgaaga agcgattaca ccgtatttac
     8461 aggcgagcgg cgctatcggc aaagcctgat tcattttctg tcatggcctt tatgccagtg
     8521 caataacgca ccggcagggc ggactacacg ctgaaactgg agatttaaca tgttagtttc
     8581 gatgaaagaa ttactgcaac ctacccgcca gcacggcttt gccattggcg catttaatgt
     8641 ggcggataac tgtttccttc gcgcagtcgt ggatgaagca gaagcgacaa atactccgac
     8701 gattattgcc atccatccca gcgaacatga ttttgtgggc gatgcctttt tcgctcacgt
     8761 tcgcgaaatt acccagcgta gtccggtccc gtttgtactg cacctcgatc atggtgcctc
     8821 tgtagagcat gttttgcgtg cgattcgctg cggatttacc tcagtaatga tcgacggttc
     8881 gctgctgcca tatgaagaaa acgtggcgct gaccgcagaa gtggttcgcc tcgctcacgc
     8941 ggttggagta tcggttgagg gtgaactggg aaccatcgga caaacaggaa cgtcggtaga
     9001 aggtggtgtt tcacaagtga cctacacaga tccggcgcag gccgccgatt ttattgctcg
     9061 tacaggtgcc gacacactgg ctgtggcgat tggtacagcg catggcatct atccaaaagg
     9121 gatgcagccg aagctgcaaa tggacatttt gcgcgacatt gccggacgta ttgatattcc
     9181 gctggtgcta cacggtggtt cggcaaaccc ggacgcggag attgctgaat cagttacgct
     9241 cggcgtcggc aaaatcaata tctccagcga tatgaaatac gcctatttcc agaaagtgcg
     9301 tgaaattctc gctaaagaga gctggtggga cccgaacgtc atctatccgg atgcgattaa
     9361 cgcagcgcgg gaagtcattc gccacaaaat gaaactgttc ggttcgctcg gtaaagcatc
     9421 actgtactaa gccagaaggg cggggtgacc cgccttttac gcaacaagga acgagatatg
     9481 cactatctgg ggttggatat tggtgggacc aaaatcgccg ccgtcgtcat ggatgcgcat
     9541 ggctgggaga ttcgccgtta ccgctgcccg acgcaaaagt cgacatatca acaatttgtc
     9601 tcatgcgttg tggcgcttat cgagcagatt aggcgggacg ttcaacgacc gatgctgaca
     9661 gggatcgcct tacccggcag tatctcgcca ctcactggcc tgattaaaaa cgcgaatatt
     9721 caggtgatta acggtcatgc gttacaggct gatttgcagc aattgcttgg gcaaccggtg
     9781 gtgatagcca atgatggtaa ctgtttcgcg ctatcagaag cttgcgacgg tgccgggcaa
     9841 gattatgacg tggtatttgg tattacgctt ggtacgggct gcggcggtgg cattgccatc
     9901 aagcaacgac cgtttatagg ggcctgggga aatgctgccg aatgcggtca tatcacgttg
     9961 ccaggctata cggagcagga agatggtcca tcagtcagtt gctattgcgg caaacacaac
    10021 tgcgtggagt cgtttgtttc cggcagcggt tttagtgaac gctatcaaca gatgactggt
    10081 aacttgctca ctcctgcggc gattgtcacc ctggcacaac gtggtgatgc ttgtgccatg
    10141 cagcaggtgg cacgttttcg ccaacagctt gcccgcacgc tggcaaccat cgttaatgtt
    10201 gttgaccctg gcgtgattgt gatcggcggc gggctttcga atgtggaact gcttatcacc
    10261 gatctgaacg cagaagtcgc tccactggtt ttcaccgatc aattcaccac tcccattgta
    10321 aaagcacagc acggcgacag tagcggaatg cgtggcgctg cctggcttgc tatgcgcaac
    10381 ggagaagcca atgaaacgtt cacaaattaa ttatgttatt gataaagcac atgccattgc
    10441 gcagaccttc cgcgtttgtt tgccagagtt cgcctatttt acggtggatg actggctgca
    10501 gcgggaacgg gataactggc aagaagtggt cgacttacag ctcggttggg atatcaccga
    10561 ttttggtcgt ggtgatttca accaaacggg actaacactg ctaacgatgc gcaacggcgc
    10621 gctggggtcg gcggcatatc caaaaccgta tgctgagaaa atgttgcaga tccaacaaga
    10681 tcagcagact ccctggcatt ttcacaccca caaaatggaa gatatcctca atcggggcgg
    10741 tggagactta tgcatgcagt tggcctgggc caacgaagcc aatctctgtg atgatcaacg
    10801 agtcattacc gtgagtgttg atggtcagcg gcgtaccatg aaacctggtg aaacgctggt
    10861 gctaaagccg gggcagggca tttgcctgcc gccgcgtctg tatcatcgct tctgggcgga
    10921 aaaagcgttt gttctcggct gggaaatctc gatggtaaat gatgaccaac acgataatta
    10981 ttttcttgag ccgggaggac gttttccggc gatagaagaa gatgaaccgg tgaaatggtt
    11041 attgtgcagt gagtatggaa ttttgcgcta ataacgggga acgctagcgc tgatatccgc
    11101 tagcgtcctt atttttactg caccggctcc gtcgacgtaa cttcaccact ggaaactacg
    11161 cccgcattca tggtttgagt cacttgctgc tttgcggcat tcacggcatg caattgcccg
    11221 gtaaccgtag gtttcagcgg gacattggcc tgaatatcgc cactggcagt aagctgaata
    11281 ttgccgtcgc cagtcaacgg taattcaggc catccccact gttgcaaaat attcaccggc
    11341 acaccgcgtc cattcaggct gatatgggtc tgacgttgtg gcgtttgtga aacactggcg
    11401 gtggcttcca gaatgccttt ttcagtaaat gcactcagtt cgctgatatt caccgtgctg
    11461 ctgttggcgg tcagcgccag cgatgggcga cgaacatcaa cacgattgaa tgtcgcggcg
    11521 gcggcattca gattcgccga accactccag acgccccatt tatgatcggt aaccagcgtc
    11581 aggttggcac cgtaaccatc gagcgcggtg agctgccacg ggaagtcagg gtcgatatca
    11641 atgatcagat tgcggctggc gctaaatctc ttcagttgca ggctatttaa ccaaccgggt
    11701 gtcgtttcca tccacaactg ttgccagttt tttggcaagg tatattccag cccggcaatt
    11761 gccgcatcat caaggagtaa cgttttcccg tcacgcaacc agctccctga cgttctgacc
    11821 ataccccctt cccagcggct ggtgaactgg cgcagcgcta cgccctgcgg ggaaaattcc
    11881 gcgtttataa tcgggtcaaa taaatgcagc gaaccataaa tgaactcgct agcgttcatc
    11941 gacagtttgc cttcctgtgt ctgccagtca tctttactga aggtcatgtt gcgcaagctg
    12001 agatcgaggt cggtcaccgc ccagtccgga ccttgcaaac gagcgtcaat gacttccagg
    12061 cgaccaatct gcaacgacgg gacagagcgt aatggcgcaa agaagtcggt tagcgatttt
    12121 tcgctttgta gacggatatc cgccatgcgc agattttcca cttgccagct gccgtcggcg
    12181 ttacgctgcg cgtttccggt taatgtcccg cgggcgatgt cggcacccaa gttagtcagc
    12241 gtaacgcgat cgttatcaat actgccttca atcagtacat tggtggcagg aacatcattg
    12301 agcgaaagcg atccggcact aaactgaatc tgcgccttcg tacccagcac tttaccggct
    12361 tctggtgacc acggaaccac gccaccattt acccgctgcg cgcttagttt ccattcgcta
    12421 ttcgggctat taaacgccat atcacgcagt tgcagacgat cggctttgaa cggtagcggc
    12481 gcggtctggt cggtgagatt cagcgtcccg ttttccagca agatggtatc gacatggcgt
    12541 ggttcggtca gttggcgact gcttagcgca atgtcgacac ttttggccac cagggtcgcg
    12601 ggctgaccat cacgaccaaa cgtgacgttc tccagcacga tatgagatgg cgcggaaaaa
    12661 cggtgatcca tcgccccgaa ggccagatga tagtcgctat tctcggaaac ccatgcgctg
    12721 atatgttctg ctccccagcg ggtttgcaga agaaaataga ggccagcgat caccactaac
    12781 agagcgatga gaatgtagag aagcagcttc ccaataaatt tcatggtctt ccatcccgtg
    12841 aaaagcacat aagggagtta tgcacgattt acgcgcaatc ctcaaggcgg gaatggtgaa
    12901 agacgtgaga ctgcggcggt aattatctta ccgccgccag tgaattactg tttttctggt
    12961 gggaaaatca gattcagcac gatggcagta ataccgcccg cggcaatccc ggaggagagc
    13021 aggtttttca gccattcagg ggcaaactgc aaaatcagcg gctgctgaga gacgcccaga
    13081 ccgaccgcca gcgacagcgc gataatcaga atcgcccgac ggttcagcgg ctcacgagaa
    13141 acgatacgca caccggaggc ggcgatggtg ccaaacatta caagcgttgc gccgcccaga
    13201 accggttcag gaatgtgttg tacaaaaccg ctcactgccg ggaacagacc cagcacgatc
    13261 aacatcagcg cgacgacaaa accgacatag cggctggcaa caccggttaa ctggatcact
    13321 ccgttgttct gcccgaagca ggagttcggg aaggtgttaa acaccgccga aacaaacgag
    13381 ttcaggccgt ttgccagcac accgcctttc aggcgtttca tgtacagcgg accggacact
    13441 ggctgttcgg aaacgtcaga ggtcgccgtg atatcgccaa tggtttccag cgaagtgatc
    13501 ataaagacca gcatcagcgg cagcagcaga ctccattcaa tgccaagacc gtaatagagc
    13561 ggcgttggca ccataatcag ttcttgtgtc atcggttcgt tgctttctgg caacatgccc
    13621 ataaaccacg ccagcgcata tccggccgcc atcgcaatta ccagtgaggc cacgcgtaag
    13681 taagggttac gttgacggtt aagcaggata attaaggcta aaaccacgcc tgccagcagc
    13741 agatttttcg gtgcgccgaa ggtgttatcg ctcatggctg cgtaaccgcc gccaatggac
    13801 gttaacccaa cctgaattag cgacaggccg ataatcatca ccacaacgcc agaaactagc
    13861 ggcgtaataa tgcggcgcgc cagatgcaga acgcgggaga tcaccatctc agtgcaactt
    13921 gccagcatca acgtgccgaa caaagccgcc atcatggtag gaacatcagc accaccggtt
    13981 ttcagcgcgg taccgcccat aatcagtggg gcaacaaagt tgaagctggt gccctgaata
    14041 gacaacagcc cggagccaac cggaccccag gccttaattt gaataatcga tgccacaccg
    14101 gaggcaaaca gcgacatact aataatgtgt tgcgtgtctt gtgccggtaa acccagcgcc
    14161 tggcagatta atagcgctgg cgtgatcacc gcaacgaaca tcgccagcag atgctgacag
    14221 gcggcaaaca gagtttgagg aagcggcgga cgatcttcaa gacggtaaat cagttcgctg
    14281 ttttgagtct gcgcaaccgg ttgcgcattt tctgactcga gggtggaaac agacatcgaa
    14341 aacaatcccg ttgtagcaaa gcgggcattt tagctgaccg aaagagaaaa gcaaacgttt
    14401 gccacggcct ctgctaaata tttaaatgag ataaatgcac ttttttatat aacttttggt
    14461 atttttctgc ttaaaatcca tgccatttgt tcattaacag ctgagaattt gacccaggaa
    14521 agcataactc gatacccgtc atagttcata agttgccact gcgcgatgaa gtatgacgag
    14581 tatgaaagag tgatgcggat acaaaggagt aactatgttt catctcgata ctttagcaac
    14641 gcttgttgcc gcaacgctga cgttgctgct cgggcgtaag ttggtccatt ccgtctcctt
    14701 tttgaagaaa tacaccatac cggaacctgt tgcgggtggt ttgttggtgg cgctggcgct
    14761 actggtactg aaaaaaagca tgggctggga agtcaacttt gatatgtccc tgcgcgatcc
    14821 gttaatgctg gcattcttcg ccaccattgg cctgaacgcc aacattgcca gtttgcgtgc
    14881 cggtgggcgt gtggttggca tcttcttgat tgtggttgtt ggtctgttgg tgatgcaaaa
    14941 tgccattggc attggtatgg ctagcctgtt agggcttgat ccgctgatgg ggctgttggc
    15001 cggttctatt acgctttccg gcggtcacgg tacgggcgct gcgtggagta aattgttcat
    15061 tgaacgctat ggcttcacca atgcgacgga agtggcgatg gcctgtgcga cgtttggtct
    15121 ggtgctgggc ggcttgattg gcggtccggt agcgcgctat ctggtgaaac actccaccac
    15181 gccgaacggt attccggatg accaggaagt cccgacggcg ttcgaaaagc cggatgtggg
    15241 ccgcatgatc acctcgctgg tgctgattga aactatcgcg ctgattgcta tctgcctgac
    15301 ggtggggaaa attgttgcgc aacttttggc tggcactgct tttgaactgc cgaccttcgt
    15361 ctgtgtactg tttgttggcg tgattctgag caacggtctg tcaatgatgg gcttttaccg
    15421 cgtctttgag cgtgcggtat ccgtgctggg taacgtaagc ctgtcgttgt tcctggcgat
    15481 ggcgctgatg gggctgaaac tatgggagct ggcttcgctg gcgctgccga tgctggcgat
    15541 tctggtggta cagaccatct tcatggcgtt gtatgccatc ttcgttacct ggcgcatgat
    15601 gggcaaaaac tacgatgcgg cagtgctggc tgcgggtcac tgtggttttg gcctcggtgc
    15661 aacgccaaca gcaatcgcca acatgcaggc gatcactgaa cgttttggcc cgtcgcacat
    15721 ggccttcctg gtagtgccga tggtcggtgc gttctttatt gatatcgtta acgcgctggt
    15781 gattaagctg tatttaatgt tgccgatttt tgccggttaa ccgatgaagc ggcggtcttc
    15841 tcaccgccgc cttttacgca ttcgagtaac gttccgtttc tggcatccag cgttctatca
    15901 gggcttttgc ctgctgtggg taacgttcgt gaatatggcg tgccaggcgc tgaacttccg
    15961 ggatcatcgc ctgatcgcgc agtaaatccg ccactttaaa ttcagcatta cccgtctgac
    16021 gcgtgcctaa caattcgcca gggccgcgaa tctccagatc tttttgcgca atcacaaacc
    16081 cgtcgttgct gtcgcgcagt acttgcagac gaatttgcgc cgttttagag agcggcgttt
    16141 tgtagagcag cacgcagtga gaagccaccg caccacgtcc cacgcgcccg cgaagctggt
    16201 gcaactgcgc tagacccaga cgctccgggt tttcgataat catcaggctg gcgttaggca
    16261 catcaacgcc gacttcaata acggtagtgg caaccagcag gtgtaactcg ccttgtttaa
    16321 atgatgccat gacggcctgc ttctcggctg gtttcatccg tccgtgtacc agaccaacat
    16381 tcaactctgg tagcgccagt ttcaactctt cccaggtagc ttccgccgcc tgcgcttcca
    16441 gtaactctga ctcttcaatc aacgtacaaa cccagtatgc ctgacgacct tcagttatgc
    16501 aggcgtggcg cacgcggtca ataatgtcgg tacggcgggt atcaggaata gcgaccgtag
    16561 tcactggcgt gcggcctggc ggcagctcat ctatcaccga ggtatcgaga tcagcatacg
    16621 cagtcattgc cagcgttcgg gggatcggcg tggcggtcat gatcaactga tgaggatgga
    16681 agccttgctg ctggcctttc tcccataacg ccagacgctg atgcacgcca aaacgatgct
    16741 gttcgtcgat aatcaccagc gccaggccgt taaactgcac ctgttcctgg aagatggcgt
    16801 gtgttccgac aatcatctgc acctgaccgc tggcgatggc ttcctgctgt gccagtcgcg
    16861 ctttaccttt ctgcttaccg gcgagccagc ccacttcgat accgagcggt gcaaaccagt
    16921 tgcggaagtt attcgcgtgc tgttcggcga gtaattcggt tggtgccatc aatgccacct
    16981 gtttgccgtg cgcaatcgca cgcagcgcgg cgagggcagc gaccagcgtt ttaccggaac
    17041 ctacatcgcc ctgtaccagg cgcatcatcg gcgcatccag cgccatatcg cgctcgatct
    17101 ccgccaccac gcgtgcctgt gcgcccgttg gcttgaacgg taaggctgcg aggagtttat
    17161 ttttcagcgc gtcattggcg ctaagcggct gggcatggaa acgttgtgca ccagcgcgta
    17221 acgccaacat gctgaggttg tgcgcaagca gttcttccag aatcagacgg cgttgtgctg
    17281 gatgctgccc ggtttcgaga tcgctaagct gtagcgtcgg cggcgggcgg tgcaaagtgc
    17341 gcaacgcttc cggtagcgtc atcattcctt gtgacagttc cggcggcagg agttcttcaa
    17401 tggcgcaggt gtcgagcaga tccagcgcct gatcggttaa tttacgcagc gtggcctgct
    17461 ttacgccttc cgttgttggg taaaccggcg ttagcgtttc ctgcaactct ggcgtactga
    17521 gatcgccctg cacgcggtat tccgggtgga tcatctccgc accatattta ccgcgctttg
    17581 cttcgccata ggccagcacg cggcggcctg tcgccaggct atttttcatt gccgcgttga
    17641 agttgaaaaa gcgcatggtg agaatgccgg aaccgtcgct gatctggcag gtcatcatcc
    17701 gccgaccgcc gaaggagata ttgcagttca gcacttcgcc ttccaccgtg gcataaacgc
    17761 ccggtagtag ttctccgatg gggtagagat gggtgcgatc ttcgtagcgc aggggaaggt
    17821 gtaagagtag atcctgcacg gtatgcaggt tgattttcgc cagcttgtta ctaagcgctg
    17881 cgccaacgcc cgttagggaa ctgagcggga cagcatctaa caggcgacct ttcatggcac
    17941 ttaccctgca gcctgcatgg tagcccacca gtcagcatcg gcttcaattt cgccttgctg
    18001 attaacgtgg gggtaaggca ggcctttgcg ttttgcgact ttcgccagca ccggatagcc
    18061 gccttcaaac agcaggcgtt gttgctctgc ttccggcaac atgctgtttt cacgcaggta
    18121 catgcctgca ttttgccgct ggcgctgggc ttcataaaga atgagggctg aggcaacgga
    18181 aacattcagc gactgcacca tgccgatcat cggtatgatg atgtcccgat ccgccagggc
    18241 caacgcttcc tgcgtgatgc ctgttttctc ctgtcccatc aaaatacagg tcgggcgagt
    18301 gtaatcaatt tcgcggaaat cgacagcgtt atcagaaaga tgggttgcca gaatctgcat
    18361 gccctggcct ttcagatgag cgacggcatc gccaatggtg cggtgcgttt tcacctgtac
    18421 ccagctgtta ctacccgcag ctgccgaagc catggtgcgc atccggctac caggccagac
    18481 agcatgaact tcatgtacac caacggcatc tgcggtacga ataatcgcag aaacgttatg
    18541 aggtttgtgg acctgctcca tgcagacggt cagatcaggc tgccgcctgg cgagcatttc
    18601 gcagatgcgt gcataacgtg ttgggttcat aaaacattaa tttcggtttc gggtgacttt
    18661 aatcacgtct ggcatcacgc ggattttgcg catgatattc gccagatgca cacggtcacg
    18721 ggcggtcaga cgaataaagg cgctgtagac gcgaccatct ttctcttccg tattcaaact
    18781 ttgaatattc gaggtggtgg tgttaattgc cgctgtcagg tttgccagcg caccctgatg
    18841 attgaacatc tccaccttga tttcggtgat gaactcctgc gccgtctctt tatcccattc
    18901 cacagccata aacttctctg gctctttctg gtagccacgg atgttacggc aggattcatg
    18961 gtggatcacc agacctttac cggggctgac gtgggcgata atcgggtcgc caggaatagg
    19021 gcggcagcat ttcgcaaagg tgatcagcac gccatcggca cctttaatgg gcagatgtcc
    19081 gtgactttgg gttgccggtg gaatggaggc gtccccatgt tgcagatttt tcgcgaccac
    19141 cacgctcatt gcgttaccaa ggccgatttc agccagcaga tcgtcaagcg ttgccagctt
    19201 catgcggtcc agctcgcgct gaatattttc ctgcgggatt tcattgagct tgcggctgcc
    19261 acccaacgca tggttgagca gacgacggcc caggcttaca gaatcatcac gcttgaggtt
    19321 tttcagcaat tgacgaattt tggcgcgcgc tttcgagcta acgacaaagt tcagccacgc
    19381 ggcattcggg cgagcgcccg gtgcggtaat aatttcaacg gtttgaccgc tggtaagcgg
    19441 ctgcgacagc gggtaaggct ggcggtcgac gcgtgcgccc acgcaggcat gaccgatatc
    19501 ggtatgcacc gcataagcga agtcgacggg cgttgcaccg gcaggcagtt cgacaatgcg
    19561 cccttctggt gtgaaaacgt aaatctcatc cgggaagaga tcggatttaa cgctctcgat
    19621 aaattcaaac gagctaccgg cactctgttg cagctccagc aggctttgca tccagcgctg
    19681 ggcgcgtatt tgcgcggtag tgctggtttc gccgtgctct ttataagccc agtgcgcggc
    19741 aacacccatc tccgccatct gatccatatc ttcggtacgg atctggacct caaccggcac
    19801 gccgtgcggg ccaatcatcg aggtgtgcaa agactgatag ccgttcgctt ttggaatggc
    19861 gatatagtct ttcacgcggc ccggacgcgg cttgtacagg ctgtgcatct ggcccagcac
    19921 gcgataacag gtgtcagaat cattgacgat cacgcggaaa gcgtagatgt ccatgattga
    19981 gtgaaaacgc tgctctttga gcaccatttt gcagtaaatc gaataaagat gcttttcgcg
    20041 accactgacg cggcacggta ttcccgcttc ctgcaaacgc ccttcgattt cagaaagaat
    20101 cttctggatc atctctttac ggttgccgcg cgcggctttc accacttctt tgattacgcg
    20161 ataacggttg ggatacagcg cctcaaaacc cagctcttcg agttcggttt taatgtggtg
    20221 gatacctaaa cggtgcgcca gcgggctgta aatttcgagg gtttcacggg cgatgcggcg
    20281 acgtttgtcc gggcgaagtg agcccagcgt gcgcatgttg tgggtacggt cggcaagttt
    20341 gatgagaatg acgcggatat cctgcaccat cgccataatc atcttgcgaa agttttcggc
    20401 ctgcgcctct ttcttatcgc ggaacttgag cttatcaagt ttcgacaccc cctctaccag
    20461 ctcggcgacg cttttaccaa aaagctgttc catgtcctgg taggtggcgg gagtatcttc
    20521 aatcacgtca tgcagcagcg ccgccataag cgtttcatag tcgagtttca tctcggccag
    20581 aatgcaggca accgccaccg ggtgcgtgat atagggttca ccgcttgaac gtgtttgccc
    20641 ctcgtgagca tcacgtgcaa cgagatacgc ctgccgcaga cgcttgattt ggtcttccgg
    20701 caggtaggtt tgaatcagtt gattcaggct ttcaaacaga tacaagggcg acccgctttg
    20761 tgattaacga cgaccttcag caatagcggt aacggcttgt aattcagcgg cttcctgctc
    20821 ttgctgttcc tggcgttcgc gaacgtcgag gatctggttg ttgatcagac cttcttcgat
    20881 ttcgcgcagc gcgattacag tggttttatc gttttcttcc ggtaccagcg gatcctttcc
    20941 gcctacctgc atctgacgag cgcgacgcgc ggcgaccagt accaggtcaa aacggttacc
    21001 aattttctct acagcgtcct gaacagttac gcgtgccata cttaaaaagc tccacaggtg
    21061 aagaaatgac tgggcatgat actgaaatca ggttcagtct gccaacaatt tgctgattaa
    21121 agcgtcatga cgctgctttt ggcggctcat gcgcagacgt tcggcgcgaa taatggtctt
    21181 caaatcggtc aacgcggtat cgaagtcatc attcacaatc aggtaatcat attcggcgta
    21241 atggctcatt tctgcaacag cttgcgccat acgctttgca atgacctctt cgctgtcctg
    21301 accgcgaccg cgtagacggc ggtccagttc aattttggac ggcggtaaaa taaagatact
    21361 ccgcgcgtgc ggcatcttct ggcgaatttg ctgcgcaccc tgccagtcga tatcgagaaa
    21421 aacatcgaca ccggtcgcca gtacttgctc aatggcctca cgcgaagtgc catagtaatt
    21481 accaaaaact tctgcgtgtt cgaggaacgc atctctgcta atcatttctt taaattcatc
    21541 atgattaaca aagaaataat gttcaccgtg gacctcacca ggacgcggtt ggcgtgtggt
    21601 gtgtgaaaca gaaacctggg tgtcatacaa cggttgggtt tttaataaag cctgaatcag
    21661 gctggattta cccgcgccac tgggggcaga aacaatataa agcgtgcctt gagccatgag
    21721 tatctttcgt atgtgattag cgaaataaag cctacatacg agcttattat acacggcgcg
    21781 gccatgtgac gtagcccttg ctcacacttt ttgcgcaaag ttgttgcgtt ttgcgtagct
    21841 gcttccgcat ttacctgtca ttcactgcaa taaagacgat tctcaggaag cgtcctcgca
    21901 gactccgttt ttgccactcg gctttgcttt catcacgttg tatatctgcc tgactatcta
    21961 cagcggaggt gatgatgaaa gtatggatgg cgatattaat aagtatcttg tgctggcaat
    22021 catcggcgtg ggcagtctgt ccggcctggt cgccagccag agcacaggaa gaaatttccc
    22081 gcctgcaaca gcaaataaaa cagtgggacg atgactactg gaaagaaggg aaaagtgagg
    22141 tggaagacgg tatttacgat cagttaagtg cccgtctcac gcagtggcaa cgctgttttg
    22201 gtgaagagac gcatcgcgac gctatgatgc cgcctttaaa cggtgcggtt atacatccgg
    22261 ttgcacatac gggtgtacgc aaaatggcgg ataaaatcgc attaagtctg tggatgcgcg
    22321 aacgtagcga tctttgggtg cagccaaaag ttgatggcgt tgcggtaacc ctggtttatc
    22381 gggacgggaa actgaacaaa gcaatcagtc gcggcaatgg cctgaaaggc gaggactgga
    22441 cgcagaaagt tcgcttaatt tccgctgtgc cgcaaaccgt tagcgggcct ttagccaaca
    22501 gtacgcttca gggggaaata tttctcaaac gcgaggggca tatccaaaaa caaatggggg
    22561 gaataaatgc ccgcgcaaaa gttgctggtc tgatgatgcg ccaggacgat agcgacacgc
    22621 tgaattctct gggcgttttt gtctgggcat ggccggatgg accgcagtta atgaccgatc
    22681 gtttaaaaga gctggctacc gctggtttta ctctaacgca gacgtatacc cgtgcggtga
    22741 aaaatgctga tgaagttgcg cgcgtacgca atgcgtggtg gaaaacgaaa ttacccttcg
    22801 tcaccgatgg cgtggttgtg cgagcggcga aagaacccga atcccgccat tggctaccag
    22861 gccaggcaga atggctggtg gcctggaaat atcaacctgt agctcgggtt gccgaagtga
    22921 aggcgattca gtttgcggtg ggtaagagcg gtaaaatatc ggtggttgcg tcactcgcac
    22981 ctgtcatgct tgatgataaa aaagtccagc gggtgaatat tggttccgtc aggcgctggc
    23041 aggagtggga tattgcgcct ggtgatcaga tcctcgtcag ccttgccggt cagggtattc
    23101 ctcgcattga tgatgtggtg tggcgcggtg cagaacgtac aaaaccgaca ccgccagaaa
    23161 accgctttaa ctcgttgacc tgctactttg cttctgatgt ttgtcaggaa cagttcattt
    23221 cacgcttagt ctggctgggg tcaaaacagg ttcttgggct ggatggcatt ggtgaggccg
    23281 gctggcgctc gctgcatcag actcatcgct ttgagcatat cttttcctgg cttttattaa
    23341 cgccagagca attacagaac acgccgggga tcgcgaaaag taaaagtgcg cagctatggc
    23401 atcagtttaa tctggctcgt aagcagcctt ttactcgctg ggtgatggca atgggaatac
    23461 cgctaacccg ggcggcgctt aatgccagtg atgaacggtc ctggtcgcaa cttttattga
    23521 gcacggagca gttctggcag cagctgccgg ggactggatc ggggcgtgtc agacaggtta
    23581 ttgaatggaa ggaaaatgcg caaatcaaga agctgggcag ttggctggct gcccagcaga
    23641 tcacaggttt tgaaccttag tgtccttcgt ggctgtagta aaagaccggt aatcccagct
    23701 taaggcgtag cgccagtaag cgggcaaaaa agccgaatac cagggtggag ataattacca
    23761 catcatggtt agaaacatag tgttgcagcg cgatgtacag cacggcggag gcaaatgaga
    23821 caccggcata caactctttc tggaatacca gcgggatgcg tttacagaac atatcgcgca
    23881 aaacgccgcc aaatacgccg gtcgtcacag ctgcgacaac ggcaatgatc gggccgtgtc
    23941 ccatatccag tgcaacctgt gcgccaatga tagaaaagac caccagtccg agcgcatcga
    24001 gcaccagaaa cactttgcgc aggtaaggca ttacaggagc aacgatagtg gtgagtactg
    24061 cggcggtagc gacaataatc acatattccg ggtgtttgac ccagccgagc ggatagtggc
    24121 ccagcagaat atcgcgcact gaccctccgc caattgccgt ggcggtcgca ataataatta
    24181 cgccaaatgt atccatgcgc cgtcgtccgg ccgccagcgc ccccgtcatg gcttcggcag
    24241 taatgccaac caaatacaaa atgtgtaaca gcatggttct ctccctaaaa atctgtctgg
    24301 cagggtagcg atttgtgcgt attgtcacga ttgagatttt ttaaggtgag aggaaattga
    24361 taacttgtag ttatgtgaaa tcgctatttt ctgtagcgta gatgcattct tctgacttct
    24421 tgtctgagaa aatgcataag aaaaaatagg ttaaaattga ggggtggaaa agaaaggtgc
    24481 aatgtttagc cattgtgttc accgcacctg gactttggct tacgcatcgg gtatctcaat
    24541 caacaaaaag agtttttagt ttctgctatt tcttctctgt aatttttaca ctgttttcca
    24601 actgcttgat gttgacctgc ggggtcggca actcctcagg catggttccg ccaagttcct
    24661 gaatggtttg cctcaccttg cgacccacgt caaagtgtgt ggtgttagct tgctgttttg
    24721 aattcacgcc atcccgcttg agtttttctt ctgtttgggt agctcgaaag agattagccg
    24781 ccagttctgt tgaacccata tgatccagga tcttctgact ctttttcagc cccttccgct
    24841 gatggatggc tttctgatct aatccaccat acagcccccg gtaaccatga ttctgaaata
    24901 tggcgaaatc gatagctgtc gctaccccag cttgctgcgc tgcctcaact aattgtttgt
    24961 tatgttcttt caactcattt ctcagaaaaa gacgcttttc gtcttcacga agttgcctga
    25021 aagcttcatc gtcagccagc tcctgccgtc gggtctggat agcaaaataa gtttgccctg
    25081 ccgcaataac cggtttcgca gggtcgccgt tttgtactac caggtaacag gcatagcggg
    25141 agagtacgat gtcttttaat gctcttttgg catttgagcc aattttgacc atatcgagga
    25201 tctcctcgaa atggtcatca attgtgtgac cactattcag acaagcttct ttggcgcgcg
    25261 ttaatacagg tataaagtga cgatactctg aataacccag tagttttcca agttgccttg
    25321 ctgaccattg ttctgctccg tttgcattaa tcagctttat ctcttcaaaa ggttgatgat
    25381 gttcgttcat tgcccactcc atgtgatatc gattgcaagg aaagtaagtc tacaactgtg
    25441 tttatataca gttgtaagta tctcacgttc tcactgcgct gggcaaaaaa acatcgcaaa
    25501 atctggagca ggcacgcaaa acagaattga atgttgcggg gaggaaaaat gccgctctgt
    25561 ttaagtgggc ggcatttgtg aaggctgaaa aggaagaatt ggcgttacga gttggatctg
    25621 ctcattgagt atatgcatca aagacttgtc attgctgggg tttctgtaat aacgattttt
    25681 attgtgtaca cccttgtgta cacggttttt tcaggtgtgc cgccgatatg ggaactgtca
    25741 gggcaaatat tcaccggaat tttttctag
//
LOCUS       DAZFLD010000055        25754 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.055, whole genome shotgun sequence.
ACCESSION   DAZFLD010000055 DAZFLD010000000
VERSION     DAZFLD010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25754)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25754
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.055"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            238..1536
                     /gene="kgtP"
                     /locus_tag="ACHKBL_001886"
     CDS             238..1536
                     /gene="kgtP"
                     /locus_tag="ACHKBL_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate permease"
                     /protein_id="HFO5818765.1"
                     /translation="MAESTVTADSKLTSSDTRRRIWAIVGASSGNLVEWFDFYVYSFC
                     SLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRKKSMLLSVCM
                     MCFGSLVIACLPGYETIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGRKGF
                     YASFQYVTLIGGQLLALLVVVVLQHTMEDAALREWGWRIPFALGAVLAVVALWLRRQL
                     DETSQQETRALKEAGSLKGLWRNRRAFIMVLGFTAAGSLCFYTFTTYMQKYLVNTAGM
                     HANVASGIMTAALFVFMLIQPLIGALSDKIGRRTSMLCFGSLAAIFTVPILSALQNVS
                     SPYAAFGLVMCALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAVANAIFGGSAEYV
                     ALSLKSIGMETAFFWYVTLMAVVAFLVSLTLHRKGKGMRL"
     gene            complement(1533..1856)
                     /gene="yfiM"
                     /locus_tag="ACHKBL_001887"
     CDS             complement(1533..1856)
                     /gene="yfiM"
                     /locus_tag="ACHKBL_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiM family lipoprotein"
                     /protein_id="HFO5818766.1"
                     /translation="MRILFVCSLLLLSGCSHMANDSWSGQDKAQHFIASAMLSAAGNE
                     YAQHQGMSRDRSAMFGLMFSVSLGASKELWDSRPEGSGWSWKDFAWDIAGASTGYTVW
                     QLTRH"
     gene            complement(1900..3255)
                     /gene="pssA"
                     /locus_tag="ACHKBL_001888"
     CDS             complement(1900..3255)
                     /gene="pssA"
                     /locus_tag="ACHKBL_001888"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HFO5818767.1"
                     /translation="MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKI
                     ASAKQRICIVALYLEQDDGGKGILNALYEAKRQRPELDVRVLVDWHRAQRGRIGAAAS
                     NTNADWYCRMAQENPGVDVPVYGVPINTREALGVLHFKGFIIDDSVLYSGASLNDVYL
                     HQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLF
                     RQELRDAAYHFQGDADNDQLSVTPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNL
                     PAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGALPYLYEINLRRFLSR
                     LQYYVNTDQLVVRLWKDDDNTYHLKGMWVDDKWMLITGNNLNPRAWRLDLENAILIHD
                     PQLELAPQREKELELIREHTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRI
                     L"
     gene            complement(3369..6029)
                     /gene="pat"
                     /locus_tag="ACHKBL_001889"
     CDS             complement(3369..6029)
                     /gene="pat"
                     /locus_tag="ACHKBL_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysine acetyltransferase"
                     /protein_id="HFO5818768.1"
                     /translation="MSQRGLEALLRPKSIAVIGASMKSNRAGYLMMRNLLAGGFNGPV
                     LPVTPAWKAVLGVLAWPDIASLPFTPDLAVLCTNASRNLALLEELGEKGCKTCIILSA
                     PASQHEDLRACALRHNMRLLGPNSLGLLAPWQGLNASFSPVPIKRGKLAFISQSAAVS
                     NTILDWAQQREMGFSYFIALGDSLDIDVDELLDYLARDSKTSAILLYLEQLSDARRFV
                     SAARSASRNKPILVIKSGRSPAAQRLLNTTAGMDPAWDAAIQRAGLLRVQDTHELFSA
                     VETLSHMRPLRGDRLMIISNGAAPAALALDALWSRNGKLATLSEETCQKLRDALPEHV
                     AISNPLDLRDDASSEHYIKTLDILLHSQDFDALMVIHSPSAAAPATESAQALIEAVKH
                     HPRSKYVSLLTNWCGEHSSQEARRLFSEAGLPTYRTPEGTITAFMHMVEYRRNQKQLR
                     ETPALPSNLTSNTAEAHLLLQQAIAEGATSLDTHEVQPILQAYGMNTLPTWIASDSTE
                     AVHIAEQIGYPVALKLRSPDIPHKSEVQGVMLYLRTANEVQQAANAIFDRVKMAWPQA
                     RIHGLLVQSMANRAGAQELRVVVEHDPVFGPLIMLGEGGVEWRPEDQAVVALPPLNMN
                     LARYLVIQGIKSKKIRARSALRPLDVAGLSQLLVQVSNLIVDCPEIQRLDIHPLLASG
                     SEFTALDVTLDIAPFEGDNESRLAVRPYPHQLEEWVELKNGERCLFRPILPEDEPQLQ
                     QFISRVTKEDLYYRYFSEINEFTHEDLANMTQIDYDREMAFVAVRRIDQTEEILGVTR
                     AISDPDNIDAEFAVLVRSDLKGLGLGRRLMEKLITYTRDHGLQRLNGITMPNNRGMVA
                     LARKLGFNVDIQLEEGIVGLTLNLAQREES"
     gene            complement(6061..6759)
                     /gene="tapT"
                     /locus_tag="ACHKBL_001890"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
     CDS             complement(6061..6759)
                     /gene="tapT"
                     /locus_tag="ACHKBL_001890"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HFO5818769.1"
                     /translation="MTENAVLQLRAERIARATRPFLARGNRVRRCQRCLLPETLCLCS
                     TITPAQAKSRFCLLMFDTEPMKPSNTGRLIADILPDTVAFQWSRTEPSLDLLDLVQNP
                     DYQPMVVFPASYADEQREVIFTPPAGKPPLFIMLDGTWPEARKMFRKSPYLDNLPVIS
                     VDLSRLSAYRLREAQAEGQYCTAEVAIALLDMAGDTEAATGLGEHFTRFKTRYLAGKT
                     QHLGSITAEQLESV"
     gene            complement(6828..7247)
                     /gene="trxC"
                     /locus_tag="ACHKBL_001891"
     CDS             complement(6828..7247)
                     /gene="trxC"
                     /locus_tag="ACHKBL_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HFO5818770.1"
                     /translation="MNTVCTHCQAINRIPDDRIEDAAKCGRCGHDLFDGEVINATGET
                     LDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAQERSGKVRFVKVNTEAERELSSRF
                     GIRSIPTIMIFKNGQVVDMLNGAVPKAPFDSWLNESL"
     gene            7454..8491
                     /gene="yfiF"
                     /locus_tag="ACHKBL_001892"
     CDS             7454..8491
                     /gene="yfiF"
                     /locus_tag="ACHKBL_001892"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HFO5818771.1"
                     /translation="MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRA
                     DGGRRPARDDKQSQPRDRKWEESPWRTVSRAPGDETPEKADHGGISGKSFIDPEVLRR
                     QRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
                     ELTKASGTEHHGGVCFLIKKRNGTTVLQWVSQAGAQDCVLALENESNPHNLGGMMRSC
                     AHFGVKGVVVQDAALLESGAAIRTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTS
                     SEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGTGNVAGLNISVATGV
                     LLGEWWRQNKA"
     gene            complement(8539..9228)
                     /gene="ung"
                     /locus_tag="ACHKBL_001893"
     CDS             complement(8539..9228)
                     /gene="ung"
                     /locus_tag="ACHKBL_001893"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFO5818772.1"
                     /translation="MANELTWHDVLAEEKQQPYFLNTLQTVASERQSGVTIYPPQKDV
                     FNAFRFTELGDVKVVILGQDPYHGPGQAHGLAFSVRPGIATPPSLLNMYKELENTIPG
                     FTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISLINQHREDVV
                     FLLWGSHAQKKGAIIDKQRHHILKAPHPSPLSAHRGFFGCNHFVLANQWLEQRGEKPI
                     DWMPVLPAESE"
     gene            9533..9916
                     /gene="grcA"
                     /locus_tag="ACHKBL_001894"
     CDS             9533..9916
                     /gene="grcA"
                     /locus_tag="ACHKBL_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007728348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HFO5818773.1"
                     /translation="MITGIQITKAANDDLLNSFWLLDSEKGEARCIVAKAGFAEDEVV
                     AVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVIARTFTESL"
     gene            complement(9972..10559)
                     /gene="eamB"
                     /locus_tag="ACHKBL_001895"
     CDS             complement(9972..10559)
                     /gene="eamB"
                     /locus_tag="ACHKBL_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/O-acetylserine transporter"
                     /protein_id="HFO5818774.1"
                     /translation="MTPTLLSAFWTYTLITAMTPGPNNILALSSATTHGFRQSTRVLA
                     GMSLGFLIVMLLCAGISFSLAVIDPAAVHLLSWAGAAYIVWLAWKIATSPTKEDGLQT
                     KPISFWASFALQFVNVKIILYGITALSTFVLPQTQALSWIIGVSVLLAMIGTFGNVCW
                     ALAGHLFQRLFRQYGRQLNIVLALLLVYCAVRIFY"
     gene            10662..11543
                     /gene="yfiE"
                     /locus_tag="ACHKBL_001896"
     CDS             10662..11543
                     /gene="yfiE"
                     /locus_tag="ACHKBL_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO5818775.1"
                     /translation="MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFS
                     VQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLREAAKKESDPDGELRVISGEMLLS
                     YRMPQVLQRFRQRAPKVRLSLQSLNCYVIRDALLNDEADVGVFYRVGNDDALNRRELG
                     EQPLVLVASPQIADVDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIEL
                     ISIESIKRCVAANIGVSYLPRFTVANELASGELIELPFGEQSQTITAMCAHHAGKAVS
                     PAMHTFIQCVEECFLPG"
     gene            complement(11576..12910)
                     /gene="srmB"
                     /locus_tag="ACHKBL_001897"
     CDS             complement(11576..12910)
                     /gene="srmB"
                     /locus_tag="ACHKBL_001897"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0000027 - ribosomal large subunit assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HFO5818776.1"
                     /translation="MTVTTFSELELDESLLEALQDKGFTRPTAIQAAAIPPALDGRDV
                     LGSAPTGTGKTAAYLLPALQHLLDFPRKKSGPPRILILTPTRELAMQVADHARELAKH
                     THLDIATITGGVAYMNHAEVFSENQDIVVATTGRLLQYIKEENFDCRAVETLILDEAD
                     RMLDMGFAQDIEHIAGETRWRKQTLLFSATLEGDAIQDFAERLLEDPVEVSANPSTRE
                     RKKIHQWYYRADDLEHKTALLVHLLKQPEATRSIVFVRKRERVHELANWLREAGINNC
                     YLEGEMVQGKRNEAIKRLTEGRVNVLVATDVAARGIDIPDVSHVFNFDMPRSGDTYLH
                     RIGRTARAGRKGTAISLVEAHDHLLLGKVGRYIEEPIKARVIDELRPKTRAPSEKQTG
                     KPSKKVLAKRAEKKKAKEKEKPRVKKRHRDTKNIGKRRKPSGTGVPPQTTEE"
     gene            13042..13779
                     /gene="trmN"
                     /locus_tag="ACHKBL_001898"
     CDS             13042..13779
                     /gene="trmN"
                     /locus_tag="ACHKBL_001898"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(1)(Val)
                     (adenine(37)-N(6))-methyltransferase TrmN"
                     /protein_id="HFO5818777.1"
                     /translation="MSQSTSVLRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAG
                     VKRCLDIGAGSGLLALMLAQRTDDSVMIDAVELESEAAAQAQENINQSPWAERINVHT
                     ADIQQWITQQTVRFDLIISNPPYYQQGVECATPQREQARYTTTLDHPSLLTCAAECIT
                     EEGFFCVVLPEQIGNGFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFS
                     DRLVIRGPDQNYSEAYTALTQAFYLFM"
     gene            complement(13764..15386)
                     /gene="nadB"
                     /locus_tag="ACHKBL_001899"
     CDS             complement(13764..15386)
                     /gene="nadB"
                     /locus_tag="ACHKBL_001899"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HFO5818778.1"
                     /translation="MNTLPEHSCDVLIIGSGAAGLSLALRLADQHQVIVLSKGPVTEG
                     STFYAQGGIAAVFDETDSIDSHVEDTLIAGAGICDRHAVEFVASNARSCVQWLIDQGV
                     LFDTHIQPNGAESYHLTREGGHSHRRILHAADATGREVETTLVSKALNHPNIRVLERS
                     NAVDLIVSDKIGLPGTRRVVGAWVWNRNKETVETCHAKAVVLATGGASKVYQYTTNPD
                     ISSGDGIAMAWRAGCRVANLEFNQFHPTALYHPQARNFLLTEALRGEGAYLKRPDGTR
                     FMPDFDDRGELAPRDIVARAIDHEMKRLGADCMFLDISHKPADFIRQHFPMIYEKLLG
                     LGIDLTQEPVPIVPAAHYTCGGVMVDDHGRTDVEGLYAIGEVSYTGLHGANRMASNSL
                     LECLVYGWSAAEDITRCMPYAHGVSTLPPWDESRVENPDERVVIQHNWHELRLFMWDY
                     VGIVRTTKRLERALRRITMLQQEIDEYYAHFRVSNNLLELRNLVQVAELIVRCAMMRK
                     ESRGLHFTLDYPELLTHSGPSILSPGNHYINR"
     gene            15642..15797
                     /gene="rseD"
                     /locus_tag="ACHKBL_001900"
     CDS             15642..15797
                     /gene="rseD"
                     /locus_tag="ACHKBL_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rpoE leader peptide RseD"
                     /protein_id="HFO5818779.1"
                     /translation="MIRLQHDKQKQMRYGTLQKRDTLTLCLLKLQLMEWRFDSAWKFG
                     LGRLYLG"
     gene            15794..16369
                     /gene="rpoE"
                     /locus_tag="ACHKBL_001901"
     CDS             15794..16369
                     /gene="rpoE"
                     /locus_tag="ACHKBL_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HFO5818780.1"
                     /translation="MSEQLTDQVLVERVQKGDQKAFNLLVVRYQHKVASLVSRYVPSG
                     DVPDVVQEAFIKAYRALDSFRGDSAFYTWLYRIAVNTAKNYLVAQGRRPPSSDVDAIE
                     AENFESGGALKEISNPENLMLSEELRQIVFRTIESLPEDLRMAITLRELDGLSYEEIA
                     AIMDCPVGTVRSRIFRAREAIDNKVQPLIRR"
     gene            16402..17052
                     /gene="rseA"
                     /locus_tag="ACHKBL_001902"
     CDS             16402..17052
                     /gene="rseA"
                     /locus_tag="ACHKBL_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016989 - sigma factor antagonist activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-E factor RseA"
                     /protein_id="HFO5818781.1"
                     /translation="MQKEQLSALMDGETLDSELLNELAHNPEMQKTWESYHLIRDSMR
                     GDTPEVLHFDISSRVMAAIEEEPVRQPATLIPEAQPAPHQWQKMPFWQKVRPWAAQLT
                     QMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANN
                     GQQQQVQEQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGIQTLGTQSQ"
     gene            17052..18008
                     /gene="rseB"
                     /locus_tag="ACHKBL_001903"
     CDS             17052..18008
                     /gene="rseB"
                     /locus_tag="ACHKBL_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB"
                     /protein_id="HFO5818782.1"
                     /translation="MKQLWFAMSLVTGSLLFSANASATPASGALLQQMNLASQSLNYE
                     LSFISINKQGVESLRYRHARLDNRPLAQLLQMDGPRREVVQRGNEISYFEPGLEPFTL
                     NGDYIVDSLPSLIYTDFKRLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYSYIVWM
                     DTESKLPMRVDLLDRDGETLEQFRVIAFNVNQDISSSMQTLAKANLPPLLSVPVGEKA
                     KFSWTPTWLPQGFSEVSSSRRPLPTMDNMPIESRLYSDGLFSFSVNVNRATPSSTDQM
                     LRTGRRTVSTSVRDNAEITIVGELPPQTAKRIAENIKFGAAQ"
     gene            18005..18484
                     /gene="rseC"
                     /locus_tag="ACHKBL_001904"
     CDS             18005..18484
                     /gene="rseC"
                     /locus_tag="ACHKBL_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR-reducing system protein RseC"
                     /protein_id="HFO5818783.1"
                     /translation="MIKEWATVVSWENGQALVSCDVKASCSSCASRAGCGSRVLNKLG
                     PQTTHTIVVPCDEPLVPGQKVELGIAEGSLLSSALLVYMSPLVGLFLIASLFQLLFAS
                     DIAALCGAILGGVGGFLIARGCSRKFAARSEWQPIILSVALPPGLVRFETSSEDASQ"
     gene            18682..20481
                     /gene="lepA"
                     /locus_tag="ACHKBL_001905"
     CDS             18682..20481
                     /gene="lepA"
                     /locus_tag="ACHKBL_001905"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HFO5818784.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
                     CSAKTGVGVQDVLERLVRDIPPPEGDPEGPLQALIIDSWFDNYLGVVSLIRIKNGTLR
                     KGDKVKVMSTGQTYNADRLGIFTPKQVDRTELKCGEVGWLVCAIKDIHGAPVGDTLTL
                     ARNPAEKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLNDASLFYEPESSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSREVIYVDSPSKLPA
                     VNNIYELREPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMAEV
                     VLDFFDRLKSTSRGYASLDYNFKRFQASDMVRVDVLINGERVDALALITHRDNSQNRG
                     RELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISRKKKLLQ
                     KQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK"
     gene            20497..21471
                     /gene="lepB"
                     /locus_tag="ACHKBL_001906"
     CDS             20497..21471
                     /gene="lepB"
                     /locus_tag="ACHKBL_001906"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFO5818785.1"
                     /translation="MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAAGD
                     SLDKATLKKVAPKPGWLETGASVFPVLAIVLIVRSFIYEPFQIPSGSMMPTLLIGDFI
                     LVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDP
                     VSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETK
                     ENGIRLSERKETLGDVTHRILTVPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNR
                     DNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGVRLSRIGGIH"
     gene            21743..22423
                     /gene="rnc"
                     /locus_tag="ACHKBL_001907"
     CDS             21743..22423
                     /gene="rnc"
                     /locus_tag="ACHKBL_001907"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HFO5818786.1"
                     /translation="MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLG
                     DSILSYVIANALYHRFPRVDEGDMSRMRATLVRGNTLAELAREFELGECLRLGPGELK
                     SGGFRRESILADTVEALIGGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRL
                     QEYLQGRHLPLPTYLVVQVRGEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQ
                     ALKKLELE"
     gene            22420..23325
                     /gene="era"
                     /locus_tag="ACHKBL_001908"
     CDS             22420..23325
                     /gene="era"
                     /locus_tag="ACHKBL_001908"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HFO5818787.1"
                     /translation="MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTR
                     HRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWT
                     PDDEMVLNKLRDGKAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGL
                     NVDTIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEI
                     ERFVSNERGGYDINGLILVEREGQKKMVIGNKGAKIKTIGIEARKDMQEMFEAPVHLE
                     LWVKVKSGWADDERALRSLGYVDDL"
     gene            23337..24065
                     /gene="recO"
                     /locus_tag="ACHKBL_001909"
     CDS             23337..24065
                     /gene="recO"
                     /locus_tag="ACHKBL_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HFO5818788.1"
                     /translation="MEGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRS
                     TLKGALQPFTPLLLRFGGRGEVKTLRSAEAVSLALPLSGITLYSGLYINELLSRVLEY
                     ETRFSELFFDYLHCIQSLAGATGTPEPALRRFELALLGHLGYGVNFTHCAGSGESVDD
                     TMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTRMALKPYLG
                     GKPLKSRELFRQFMPKRTVKTHYE"
     gene            24077..24808
                     /gene="pdxJ"
                     /locus_tag="ACHKBL_001910"
     CDS             24077..24808
                     /gene="pdxJ"
                     /locus_tag="ACHKBL_001910"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HFO5818789.1"
                     /translation="MAELLLGVNIDHIATLRNARGTAYPDPVQAAFIAEQAGADGITV
                     HLREDRRHITDRDVRILRQTLDTRMNLEMAVTEEMLAIAVETKPHFCCLVPEKRQEVT
                     TEGGLDVAGQRDKMRDACKRLAEAGIPVSLFIDADEEQIKAAAEVGAPFIEIHTGCYA
                     DAKTDAEQAQELARIAKAATFAASLGLKVNAGHGLTYHNVKAIAAIPEMHELNIGHAI
                     IGRAVMTGLKDAVAEMKRLMLEARG"
     gene            24808..25188
                     /gene="acpS"
                     /locus_tag="ACHKBL_001911"
     CDS             24808..25188
                     /gene="acpS"
                     /locus_tag="ACHKBL_001911"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HFO5818790.1"
                     /translation="MAILGLGTDIVEIARIEAVIARSGERLARRVLSDNEWAIWKTHH
                     QPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDELGKPRLRLWGEALKLAEKL
                     GVVNMHVTLADERHYACATVIIES"
     gene            25610..25690
                     /gene="shoB"
                     /locus_tag="ACHKBL_001912"
     CDS             25610..25690
                     /gene="shoB"
                     /locus_tag="ACHKBL_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin ShoB"
                     /protein_id="HFO5818791.1"
                     /translation="MTDCRYLIKRIIKIIIAVLQLILLFL"
BASE COUNT         6313 a         6411 c         6793 g         6237 t
ORIGIN      
        1 ctcccacata agctagattc agcagcgaat acggcttccc caacgtgccc acctccatac
       61 gtgtcctcct taccagaaat ttatccttaa gctcctcaat aaccattttc ctgctaacta
      121 aattcgtggt taaggttgca taatgatatg caacaaatgt ataacatttt ctttaccaat
      181 agaaataaac aaaagcgacc gacaaaagca tcggattacg gcaggagaca taatggcatg
      241 gctgaaagta ctgtaacggc agacagcaaa ctgacaagta gtgatactcg tcgccgcatt
      301 tgggcaattg tgggggcctc ttcaggtaat ctggtcgagt ggttcgattt ctatgtctac
      361 tcgttctgtt cactctactt cgcccacatc ttcttccctt ccgggaacac gacgactcaa
      421 ctactgcaaa cagcaggtgt ttttgctgcg ggattcctga tgcgcccaat aggcggttgg
      481 ctatttggcc gcatagccga taaacatggt cgcaaaaaat cgatgctgtt atcggtgtgt
      541 atgatgtgtt tcggatcgct ggttatcgcc tgcctcccag gttatgaaac tataggtacg
      601 tgggctccgg cattattgct tctcgctcgt ttatttcagg gattatccgt tggcggagaa
      661 tatggcacca gcgccaccta tatgagtgaa gttgccgttg aagggcgcaa aggtttttac
      721 gcatcatttc agtatgtgac gttgatcggc gggcaactgc tagctctact ggttgtcgtg
      781 gttttacaac acaccatgga agacgctgca cttagagagt ggggatggcg tattcctttc
      841 gcgttaggag ctgtgttagc tgttgtggcg ttgtggttac gtcgtcagtt agatgaaact
      901 tcacaacaag aaacacgcgc tttaaaagaa gctggatctc tgaaaggatt atggcgcaat
      961 cgccgtgcat tcatcatggt tctcggtttt accgctgcgg gctccctttg tttctatacc
     1021 ttcaccactt atatgcagaa gtatctggta aatactgcgg gaatgcatgc caacgtggcg
     1081 agtggcatta tgactgccgc attgtttgta ttcatgctta ttcaaccact tattggcgcg
     1141 ctgtcggata agattggtcg tcgtacctca atgttatgtt tcggttctct ggcagccatt
     1201 tttaccgttc ctattctctc agcattgcag aacgtttcct cgccttatgc cgcttttggt
     1261 ctggtgatgt gcgccctgct gatagtgagt ttttatactt caatcagtgg aatactgaag
     1321 gctgagatgt tcccggcaca ggttcgcgca ttaggcgttg gtctgtcata tgcggtcgcc
     1381 aatgctatat ttggtgggtc ggcggagtac gtagcgttgt cgctgaaatc aataggaatg
     1441 gaaacagcct tcttctggta tgtgaccttg atggccgtgg tggcatttct ggtttcacta
     1501 acattgcacc gtaaaggaaa agggatgcgg ctttagtgac gggtcagttg ccagacggta
     1561 tagccggtgc ttgcaccggc gatatcccag gcaaaatcct tccagctcca gccgctcccc
     1621 tcagggcggc tatcccaaag ctcttttgac gcccccagac tgacagagaa catcaatcca
     1681 aacatggcac tgcgatcccg gctcatcccc tgatgctgtg catattcatt tccggcggcg
     1741 gaaagcatcg ccgaggcgat aaagtgttga gctttatcct gcccgctcca gctatcgtta
     1801 gccatatggc tgcatccaga aagtaacaat agcgaacaga caaaaagaat acgcaaaacg
     1861 tggcctccaa aacaaacccc gtacaggacg gggttgtgat tacaggatgc ggctaattaa
     1921 tcggtcgatg cggatacggc gcaaacgacg gatgagttta cgaaccttca ccggataatc
     1981 ggcaatactt tgcagatcgc gatagtgctt aacgatggtg gtatgctcgc ggatcagctc
     2041 cagttctttc tctcgctgtg gcgcaagctc cagttgcgga tcgtggatca aaatggcgtt
     2101 ttccagatcc agacgccagg cgcgcgggtt caggttatta ccggtgatca acatccactt
     2161 atcatcaacc cacatccctt ttaggtgata ggtgttgtcg tcatctttcc ataaccgaac
     2221 cactagctgg tcagtattga cgtaatactg caaacggctc aggaaacgac gcagattgat
     2281 ctcatagaga taaggcaatg cgccaattat cttgaaaggt tcatcttccg gaatgtagaa
     2341 gtcgttcgcg gttttatcac caacaataat ttcgaccttt ttcccttcgc gcagcaactg
     2401 gataatattg cgcacaagga ttgctggcag gttgaagtat ggcgtacaga tggttagttt
     2461 ctgctcggca caaggcataa gatggaaaat ggtcttgttc aacagactcg atttccccag
     2521 ccccactagc ggcgttacgg aaagctgatc gttgtcggca tcgccctgga aatgataagc
     2581 ggcatcacgc agctcctggc ggaacaggcg aatgtcgttc ttgatttccg ggctttttgg
     2641 ccgattaaca tcatccagac gattaacgcc gcggccattc ataatattct gtgtaaccca
     2701 ttcaaacata atgtctgaca tcttacggtt acggattaga tgataacggt cgtagcgata
     2761 tttatcgtgc tgatgcaggt aaacatcgtt caggctggca ccgctataaa gtacgctatc
     2821 gtcgatgata aagcctttaa agtgcagaac accaagggct tcacgggtat tgattggaac
     2881 gccataaacc ggaacatcta cgcccggatt ttcctgcgcc atgcggcagt accagtcagc
     2941 gttagtgtta gatgccgcag cgccaatgcg tccacgttgt gcacgatgcc agtcgaccag
     3001 cacccgcaca tccagttccg gacgctgcct tttagcctca tacaacgcgt tcagaatgcc
     3061 tttgccaccg tcatcctgtt cgagatacag ggcaacaatg caaatgcgct gcttcgcgct
     3121 ggctattttt tccagcaacg tctcccggaa gtcggcggga gcgtaaaaga aatcgacatc
     3181 atcaactgat tgagaaatct tgggtagttg ggcaaggtgt tgttgatgtt tattacgctt
     3241 aaattttgac aacatcacag tgcatttctt ctctgttcat tgaagggtcc tctgtgcaat
     3301 gcagacgaca taagcgggca ataataacac cagtcccgat taagtggtca acatttccag
     3361 taccttactc atgattcctc gcgctgggca agatttagcg taagcccaac gatcccctct
     3421 tcgagctgga tatcaacgtt aaacccgagc ttgcgggcca gcgccaccat gccacgattg
     3481 tttggcatcg taataccatt cagacgttgt agtccgtgat ctcgcgtata ggtaatcaac
     3541 ttttccatta agcgtcgacc taagcctaac cctttgagat ccgagcgaac cagcacggca
     3601 aattcggcat cgatgttatc agggtcggag atcgcacgcg tgacgccgag gatctcttct
     3661 gtttgatcaa tacgtcgtac cgccacaaaa gccatttccc gatcgtagtc gatctgcgtc
     3721 atgttggcta aatcttcatg ggtaaattcg ttgatctcgc taaagtagcg gtaataaaga
     3781 tcttctttgg tgactcgcga aatgaattgc tgaagctgtg gctcatcttc tggcaaaatc
     3841 gggcggaaca agcagcgttc accgtttttc aattctaccc attcttccag ctgatgcgga
     3901 taagggcgca ctgccagccg actctcgtta tcgccttcaa acggcgcgat atccagcgtg
     3961 acatccagcg cggtaaattc actgccagaa gccagcaaag gatgaatatc cagacgctga
     4021 atttccgggc aatcgacaat caagttagaa acctgcacca gaagctggct caagcctgca
     4081 acatctaatg ggcgtagcgc actgcgcgca cgaatctttt tacttttgat cccctgaata
     4141 accagatagc gggccaggtt catgttcagc ggcggtagtg cgacgacggc ttgatcttca
     4201 ggacgccact ccacaccgcc ttcgcccagc atgatcaacg gcccgaaaac cggatcgtgc
     4261 tcaaccacaa cccgcaactc ctgagcgcca gcacggttag ccatactttg caccaacagg
     4321 ccgtggatcc gcgcctgcgg ccaggccatt tttacgcgat cgaaaatagc gtttgccgct
     4381 tgctggactt cattagctgt acgcaggtag agcattacgc cctgaacttc cgatttatgt
     4441 ggaatatccg gcgaacgcaa tttcagcgcc accggataac caatctgttc ggcaatatgc
     4501 accgcttcgg tgctatcgct ggcaatccag gtggggagcg tgttcatgcc atacgcttgc
     4561 aggatgggct gaacttcatg ggtatcgagc gacgtagccc cttcggcaat cgcctgttgc
     4621 aacagaagat gcgcttctgc ggtattagag gtcagattgc tgggcaacgc cggcgtttcg
     4681 cgtagttgct tctgattacg tcggtactcc accatatgca taaaagcagt gatggttcct
     4741 tccggcgtgc gataggtcgg cagcccggct tcactgaata atcgtcgtgc ctcttgcgag
     4801 gagtgctcgc cgcaccagtt cgtcagcaga gaaacatact tgctgcgggg atgatgcttt
     4861 accgcttcaa ttaatgcttg cgcactttct gttgcgggag cagcggcgct gggcgaatga
     4921 ataaccatca gcgcgtcaaa atcctggctg tggagcagaa tatccagcgt tttaatatag
     4981 tgctcactgc tggcgtcatc gcgtagatcg agcgggttag agattgccac atgttctggc
     5041 agtgcatcgc gcagtttctg gcaggtttct tcgcttagcg ttgccagctt gccattgcgt
     5101 gaccataagg catccagcgc cagcgcggca ggcgcagcac cgttgctgat aatcatcagc
     5161 cggtcgccac gcagcgggcg catatggctg agggtttcca ccgccgaaaa cagctcgtga
     5221 gtatcctgta cccgcaacaa accggcacgc tgaatagccg catcccacgc cggatccatt
     5281 cctgccgtgg tgttcaacag acgttgagcc gccgggctac gtccgctttt aatcaccaga
     5341 atcggtttgt tgcgagaggc actacgggcc gccgaaacaa agcgtcgtgc gtcgcttaac
     5401 tgttcgagat agagcaggat ggcgctggtt ttactgtcgc gggccagata gtcgagcaat
     5461 tcatcaacgt cgatatccag gctgtcgccg agcgcaataa agtaggaaaa gcccatctca
     5521 cgctgttgcg cccagtcgag gatggtattg gagacggcag ccgattgcga aataaacgcc
     5581 agcttgccgc gtttaatcgg cacaggcgaa aagctggcat tcagaccttg ccagggagcc
     5641 agtaacccca gactgtttgg tcctagcagg cgcatgttat ggcgcagggc gcaggcgcgg
     5701 agatcttcat gttgcgatgc cggggcggaa agaataatgc aggttttaca gcctttctcg
     5761 ccgagctctt ccagaagagc aagattacgg ctggcattgg tacataaaac cgcaaggtcg
     5821 ggtgtaaagg gcaagctggc aatatccggc caggccaaca cacccaacac cgctttccag
     5881 gctggcgtca ccgggagtac cggtccgtta aagcctcccg ccagcaggtt acgcatcatc
     5941 aggtaacctg ctcgattgga tttcatcgat gcgccaatta ccgcaatcga ttttggtcgc
     6001 agtagtgctt ccagtcctcg ctgactcata ccggtctccc gcagaagtga ccgaatgatt
     6061 ttaaacgctt tctaactgtt ctgctgtgat gctacccaga tgttgcgttt ttcctgccag
     6121 atagcgcgtt ttaaaacgag tgaaatgctc gcctaagccc gttgcggctt cggtatcgcc
     6181 ggccatatct aacagtgcga tggctacctc ggcagtacaa tattggcctt cagcctgggc
     6241 ttcacgcagg cgataggcag aaagccggga aagatcgacg gaaatgacgg gaagattatc
     6301 cagatacgga cttttacgaa acatcttgcg agcttcaggc caggtaccat cgagcatgat
     6361 aaatagcgga ggcttaccgg caggtggtgt gaagatcact tcccgttgct catcagcata
     6421 cgaggcggga aagaccacca ttggctgata gtccgggttt tgtaccagat ccagcaaatc
     6481 tagcgagggt tcggtacgcg accattgaaa cgcaacggta tcaggcaaaa tatcagcaat
     6541 cagacgcccg gtattactgg gcttcattgg ctcggtgtcg aacatcagca aacagaagcg
     6601 actttttgct tgtgctgggg taattgtcga acagagacat aatgtctctg gcaaaagaca
     6661 gcgttggcag cgacgaacgc gattaccgcg ggcaagaaaa ggacgtgttg cgcgcgcaat
     6721 acgctcggcg cgtaactgga gaacagcgtt ttcggtcata agagagcgtc gaaaaaacgc
     6781 cattgtcgca gaggagaaaa cggggcacaa gatgcgcccc ggtaagatta aagagattcg
     6841 ttcagccagc tatcaaacgg cgctttcggt actgcgccat taagcatgtc gacaacctga
     6901 ccgtttttga aaatcatgat cgtcggtata ctacgaattc caaagcgact gctcaattca
     6961 cgttcagctt cggtattcac tttcacaaag cgcactttac cgctacgctc ttgcgcgaca
     7021 tcttcaaaaa ttggtgcgaa attacggcag gggccgcacc acggtgccca gaagtcgatc
     7081 accacaggta gatcatcttt cagcaatttg tcgagcgttt caccggtcgc attaatcacc
     7141 tctccgtcaa acaagtcgtg accgcagcgt ccgcattttg ccgcatcttc gatccgatcg
     7201 tcgggaatgc gattgatggc ctgacaatgg gtacaaacgg tattcataac taacctcggg
     7261 atgagtaagg agacttatcg gcaacgacgc cggtatgttt ctaatatgtt acatattatc
     7321 ggggacattg ttccagacaa caatctgttt tattcaatag gtatgatagt cgcaggcttt
     7381 ggtacgaaat catggcgaac cgcgcggact tcgggtaatc tgcgcgcttc gcgcagcgct
     7441 ggtggagaaa agcatgaacg atgaaatgaa aggtaaaagc ggcaaggtca aagtgatgta
     7501 tgtccgcagt gatgatgatt ctgataaacg tacccacaac ccgcgtaccg ggaaaggggg
     7561 cgggcgtcca ggaaaatctc gtgctgacgg tggccgtcgc cccgcccgcg atgacaaaca
     7621 gagtcagccc cgtgaccgca agtgggaaga gtcgccgtgg cgcacagttt cccgcgcgcc
     7681 gggtgatgag acgccggaaa aggccgatca cggtggcatc agtggtaaaa gttttattga
     7741 tccggaagtg ttgcgtcgtc agcgtgcgga agaaacccgc gtctacggcg aaaatgcctg
     7801 tcaggcactg ttccagagcc gtccggaagc gattgttcgc gcctggttta tccagagtgt
     7861 aacgccgcgt tttaaagaag ccttgcgctg gatggcagca aaccgcaaag cgtaccatgt
     7921 ggtagatgaa gcggaactga caaaagcgtc aggcacggaa catcacggcg gcgtttgctt
     7981 cctgatcaaa aagcgtaacg gtacaaccgt gctgcagtgg gtaagccagg caggcgcgca
     8041 ggattgtgta ctggcgctgg aaaacgaatc taacccgcat aacctgggtg gcatgatgcg
     8101 cagctgcgcg cacttcggcg tgaaaggcgt tgtggtgcag gatgcggcac tgctggagtc
     8161 gggggcggct atccgtaccg cagaaggcgg cgcagagcac gttcagccga ttactggcga
     8221 caacattgtt aacgtgctgg atgatttccg tcaggcaggt tacaccgtag tgaccacttc
     8281 cagcgagcag gggaaaccgc tgttcaaaac cagtctgcca gcgaaaatgg tactggtgct
     8341 gggtcaggaa tatgaagggt taccggatgc cgcacgcgat ccgaacgatt tgcgcgtgaa
     8401 gattgatggt actggcaacg ttgccgggct aaatatctct gttgcaaccg gcgttttgct
     8461 tggtgaatgg tggcgtcaga ataaagcctg ataaatcagc cgggtggcaa cgctgccatc
     8521 cggcgttttc ctgcaaattt actcactttc tgccggtaat actggcatcc agtcaatcgg
     8581 tttctcgcca cgttgttcca gccactgatt tgccagcaca aaatggttgc agccaaagaa
     8641 cccacgatgc gcagaaagcg gtgatggatg cggcgctttc agtatgtgat ggcgttgctt
     8701 atcaataatc gcccctttct tttgcgcatg tgagccccac aacaaaaaca ccacgtcttc
     8761 gcggtgctgg ttaatcaggc tgattacttt atcggtgaag gtctcccagc cgaggctggc
     8821 gtgggaatgt gcctgacctg cgcgaaccgt caacacagta ttgagtagca gaacgccctg
     8881 acgcgcccag ctttcaagat aaccatgatt agggcgggtg aaaccgggaa tagtattttc
     8941 cagctcttta tacatattca gcagtgacgg cggtgtcgca atgccggggc gaacggaaaa
     9001 tgccagacca tgcgcctgtc ccggtccgtg ataaggatcc tggccgagaa tcaccacttt
     9061 aacgtcaccc aactctgtaa agcggaacgc gttaaagaca tctttttgtg gcggatagat
     9121 agtgacgccg gactgccgct cgctggcgac ggtctgtagg gtattaagaa aatagggttg
     9181 ctgcttctct tcagccagca catcatgcca ggttaattcg tttgccatct cgctctcccg
     9241 cgaatcttca atccgcctag cttaactgct tctttctaca gaacaaaatc atgctcattt
     9301 tttagactta aacgagtgca aaaaaatgta aattggtaaa atttcacacc tgcataagtt
     9361 gatgtaaaac aataaaatcc atttgcaacc atacttgatg tgtggttttt attgatttaa
     9421 atcaaagatt caagggtgtt tgaggagtat atatacactc aagcaacaat ggttttacca
     9481 attggccgcg acaggctgaa caaatcaaat aattttgccg gggaggcatc acatgattac
     9541 aggtatccag attactaaag ccgctaacga cgatctgctg aactctttct ggctgctgga
     9601 cagcgaaaaa ggcgaagcgc gttgcatcgt tgcgaaagca ggttttgcag aagacgaagt
     9661 ggttgcagta agcaaactgg gtgacattga gtaccgtgaa gttccagtag aagtgaaacc
     9721 agaagttcgc gttgaaggtg gtcaacatct gaacgtaaac gttctgcgtc gtgaaactct
     9781 ggaagatgca gttaagcatc cggaaaaata tccgcagctg accatccgtg tatccggtta
     9841 tgcagttcgc tttaactctc tgactccgga acagcagcgc gacgttattg ctcgtacctt
     9901 tactgaaagc ctgtaaggct taagtagcaa gttactaagc ggaagagggc gacttcttcc
     9961 gctttttttc gttaatagaa aatgcgtacc gcgcaataga ccagcagcag cgcgagcacg
    10021 atatttaact ggcgaccata ctggcgaaac aatcgctgaa acaggtgccc cgccagcgcc
    10081 cagcacacat tgccaaacgt cccaatcatc gccagcaaaa cgctgacgcc aattatccag
    10141 cttagcgcct gcgtttgcgg caggacaaac gtcgacagtg ctgtaatgcc atacaaaatg
    10201 attttgacgt tcacaaactg caaagcaaag ctggcccaaa aactgattgg ttttgtctga
    10261 agcccatctt cctttgttgg actggtagcg attttccacg ccagccagac aatatatgcc
    10321 gcccccgccc aactcaacag atgcactgct gccgggtcga taacagccag tgaaaacgaa
    10381 attcccgcgc atagcaacat gacgattaaa aatcccagac tcatccctgc cagcacgcga
    10441 gtactctgac gaaatccgtg cgttgtggct gagctaaggg cgagaatatt gttcggtcct
    10501 ggcgtcatgg cggtaatcag ggtgtaagtc caaaaagcac ttaaaagggt cggtgtcact
    10561 tgttatctcc tctgttttgg tagacagtac ccgacacgga acatttaaaa aaatgaatgt
    10621 ttttacattc taaattcgat attttcgata ggtttggggt tatggatctg cgccgtttta
    10681 ttacacttaa aaccgtggtg gaagagggtt cctttttgcg agcttcgcaa aaattgtgct
    10741 gcacacaatc gacggtgact tttcatattc agcagcttga gcaggagttc tcagtccagt
    10801 tatttgagaa aattggtcga cgaatgtgcc ttacccgcga aggaaaaaag ctgctgccgc
    10861 acatttatga actcaccaga gtgatggata cccttcgtga ggctgccaaa aaagagtcgg
    10921 atccggacgg ggaactacgc gttatttcag gcgaaatgtt gctctcttat cgaatgccgc
    10981 aggtgttgca gcgttttcgg caacgcgctc caaaagtgcg tttatcgttg cagtcgctga
    11041 actgttacgt gatccgtgat gcgctactga atgatgaggc cgatgtcggg gttttttatc
    11101 gcgtagggaa tgatgatgct ttgaaccgac gagagttggg tgaacaaccg ctggtgctgg
    11161 tggcttcacc gcaaattgca gatgtcgatt ttactgagcc gggaagacat aacgcctgta
    11221 gctttattat caacgaacca caatgtgtct tccggcagat atttgagagc acgctgcgcc
    11281 agcgacggat cacggtggaa aacaccattg agcttataag tatcgaaagc atcaagcgtt
    11341 gtgtcgcggc aaatatcggg gtcagttacc tgccgcgctt tacagtggca aatgaacttg
    11401 cgagtggcga attaatcgaa ttgccttttg gcgaacagtc gcagaccatt acggccatgt
    11461 gcgcacacca tgctggaaaa gcggttagcc cggcgatgca cacttttatt cagtgtgttg
    11521 aagagtgttt tttgccggga taaaaacaat gccgggctta aacccggcat tgagattact
    11581 cttctgtcgt ttgcggtggc acgcccgttc cgcttggttt acggcgctta ccaatatttt
    11641 tagtgtcgcg atggcgtttt ttcacccgcg gcttctcttt ctctttagct tttttcttct
    11701 cggcacgttt agccaacact ttcttcgatg gcttgccggt ctgcttctcg cttggcgcac
    11761 gcgttttcgg acgtaactca tcaattacgc gagctttaat tggctcttca atataacggc
    11821 caactttgcc cagcagcaga tggtcatggg cttccaccag cgaaatcgcg gtgcctttac
    11881 gaccggcgcg cgcggtacgt ccgatacggt gcaaataagt atcgccactg cgcggcatat
    11941 cgaagttaaa gacgtggctg acgtcaggaa tgtcgatacc gcgcgcggca acatcggtag
    12001 caaccagtac gtttacgcgg ccttcggtca agcgtttaat cgcttcgtta cgcttgcctt
    12061 gtaccatctc accttcgaga tagcagttgt tgatgcctgc ttcgcgcagc cagtttgcca
    12121 gctcatgcac acgctcacgc ttacgcacaa acacaattga gcgggtcgct tccggctgtt
    12181 ttaacagatg caccagcaac gcggttttat gctcaagatc atcggcgcgg taataccact
    12241 gatgaatttt tttgcgctca cgggtggacg gattggcaga aacctccacc ggatcttcca
    12301 gcagacgctc ggcaaagtcc tgaatcgcat cgccttccag agttgccgaa aagagcaggg
    12361 tctgtttacg ccagcgcgtt tcgccagcaa tatgttcgat atcctgggcg aagcccatat
    12421 ccagcatacg gtctgcttcg tcgaggatca gcgtttcaac cgcgcggcaa tcgaagttct
    12481 cttcttttat gtattgcagc agacgtccgg tcgtggcgac cacgatgtcc tgattttcgc
    12541 tgaacacttc cgcgtggttc atataggcta cgccgccggt gatggtggca atatccagat
    12601 gcgtatgttt cgccagttcg cgggcatgat cggccacctg catcgccagc tcgcgagttg
    12661 gggtgaggat caaaatacgc ggcggaccgg atttcttacg tgggaaatcg agcaggtgct
    12721 gcaacgctgg cagcagatac gccgccgttt taccggtgcc tgtcggcgca gaaccgagta
    12781 catcacggcc atcgagcgca ggcggaatgg cggcagcctg aatggcggtc gggcgagtga
    12841 aacctttatc ctggagggct tccagcaggc tttcgtcgag ttcaagttcg gaaaaagtcg
    12901 ttacagtcat gttctacctc tgtgtggggc gctgattata gacgttacgg ctgcaatctt
    12961 catctgtttg tatggatatc gcttctcggg tattgttctc acccggtgct atcgccgttt
    13021 ttcctgcaag gttttatttt catgtcacag tctacatccg tgcttcgtcg taatggattt
    13081 acttttaaac agttttttgt tgctcacgat cgttgtgcga tgaaagtggg aacggatggc
    13141 attttgctgg gcgcatgggc accggtggct ggggtaaaac gttgccttga tatcggcgcg
    13201 ggtagcgggt tgctggcatt aatgctggcg cagcgaaccg atgacagcgt aatgattgat
    13261 gcagttgaac tggaaagtga agctgccgcg caggcgcagg aaaatatcaa ccagtccccg
    13321 tgggcagagc ggattaacgt ccatacggcg gatattcagc agtggatcac acagcagaca
    13381 gtacgcttcg atttaatcat cagtaaccca ccttactatc agcagggagt agagtgcgcg
    13441 acacctcaac gggaacaggc tcgctatacc actacgcttg atcacccatc gttgctgacc
    13501 tgtgcggcgg agtgcattac cgaagaggga tttttctgcg tggtgctgcc agagcagatt
    13561 ggtaatggtt ttactgaact ggcattaagt atgggctggc atttacgttt gcggacggat
    13621 gtggcggaaa acgaagcgcg actgccgcat cgggtgctgc tggcattctc cccgcaggcg
    13681 ggagaatgct ttagcgatcg cttagtcatt cgtgggccag accagaacta ttccgaagcg
    13741 tatacggcgc tgacccaggc tttttatctg tttatgtaat gattgccggg ggaaaggatc
    13801 gacggaccgg aatgggtgag cagttccgga taatccagcg tgaaatgcaa cccccgactc
    13861 tctttacgca tcattgcaca gcgaacaatc aactcggcaa cctgtaccag attacgcagc
    13921 tccagcaaat tatttgagac gcggaaatgg gcgtaatatt cgtctatttc ttgctggagc
    13981 atggttatcc gccgcagggc gcgttccagg cgcttcgttg tgcgcacaat gccgacgtaa
    14041 tcccacataa acagacgtag ctcgtgccag ttatgttgaa tcaccacccg ttcatcaggg
    14101 ttctcaacgc ggctttcatc ccacggcggt aacgtactga cgccgtgggc ataaggcata
    14161 catctggtga tatcttctgc cgccgaccag ccatagacca gacactccag caaggagtta
    14221 gaggccatgc ggttagcgcc gtgtaagccg gtataactca cttcgccaat ggcatacaaa
    14281 ccctcgacgt ccgtacgccc atgatcatcc accatcacac caccgcaggt atagtgtgca
    14341 gcaggcacaa tcggtactgg ttcttgtgtg agatcaatcc ccagcccgag caatttttca
    14401 taaatcatcg ggaaatgctg gcgaataaaa tcggcgggct tatggctgat gtcgaggaac
    14461 atacaatccg cgccgaggcg tttcatttca tggtcaatag cgcgggcgac aatatcgcgc
    14521 ggggccagtt cgccgcgatc atcaaaatcg ggcataaaac gcgtaccatc cgggcgcttg
    14581 agataagcgc cttcgccgcg cagtgcttct gttaacagga aattgcgtgc ctgtgggtga
    14641 tacagtgcgg tagggtggaa ctgattaaat tcgagattgg caacccggca gcctgcgcgc
    14701 cacgccatag caatgccatc gccagaagaa atatccggat tggtggtgta ctgataaacc
    14761 ttcgacgcac cgccggttgc cagtaccact gcttttgcgt ggcaggtttc caccgtttct
    14821 ttatttcggt tccataccca cgcgccaaca acccgtcgcg tgcccggcag gccaattttg
    14881 tcagaaacaa tcagatcaac cgcgttgctg cgctccagca cgcgaatatt cggatggttc
    14941 agcgccttgc tcaccagcgt ggtttctact tctctaccgg tggcgtcggc ggcatgaaga
    15001 attcgacggt gactatgtcc accttcacgg gtcagatggt agctttctgc accattcggt
    15061 tgaatgtggg tatcaaacaa caccccctgg tcgattagcc attgcacaca ggatcgtgca
    15121 ttgctggcga caaattcaac tgcatgacga tcgcaaatac cagccccggc aatcaatgtg
    15181 tcttccacat gcgagtcaat gctgtcagtc tcatcaaaca cggcggctat accgccctgg
    15241 gcataaaatg ttgaaccttc cgttaccggg cctttactta gaacgatgac ctgatgctgg
    15301 tcagccaggc gtagcgccag tgaaagtcca gctgcgccgc taccgataat caacacgtca
    15361 catgaatgtt cagggagagt attcattttc tttgtttaat ttactaaaca tggtttggtc
    15421 agcatagcat catgttgtgc gattaaacac ctgctatttt aatatttgtt acagtgacta
    15481 aacacgctga ctcagggcgg cgagaaaaag agaagttact ggctggtgga ggattaggtg
    15541 gtgaaataaa aaggccgttg ggttactctt caggcagtta aatgggcatt tctacacaga
    15601 taatgcgatg ttcagattct gtagacttat aatgatagat aatgatccgt ctacagcatg
    15661 acaaacaaaa acagatgcgt tacggaactt tacaaaaacg agacactcta accctttgct
    15721 tgctcaaatt gcagctaatg gagtggcgtt tcgatagcgc gtggaaattt ggtttgggga
    15781 gactttacct cggatgagcg agcagttaac ggaccaggtc ctggttgaac gggtccagaa
    15841 gggagatcag aaagccttta acttactggt agtgcgctat cagcataaag tggcgagtct
    15901 ggtttcccgc tatgtgccgt cgggtgatgt tcccgatgtg gtacaagaag cttttattaa
    15961 agcctatcgt gcgctggatt cgttccgggg agatagcgct ttttatacat ggctgtatcg
    16021 gattgctgta aatacagcga aaaattacct ggttgctcag gggcgtcgtc caccttccag
    16081 tgatgtggat gccattgaag ctgaaaactt cgaaagtggc ggcgcgttga aagaaatttc
    16141 gaaccctgag aacttaatgt tgtcagaaga actgagacag atagttttcc gaactattga
    16201 gtccctcccg gaagatttac gcatggcaat aaccttgcgg gagctggatg gcctgagcta
    16261 tgaagagata gccgctatca tggattgtcc ggtaggtacg gtgcgttcac gtatcttccg
    16321 agcgagggaa gctattgata acaaagttca accgcttatc aggcgttgac gatagcggga
    16381 tactggataa gggtattagg catgcagaaa gaacaacttt ccgctttaat ggatggcgaa
    16441 acgctggata gtgagctgct taacgaactg gctcataacc cagaaatgca gaaaacctgg
    16501 gaaagctatc acttaatccg tgactcaatg cggggtgata ctcccgaggt gctccatttc
    16561 gatatctctt cacgcgtgat ggccgccatt gaagaagagc cagtacgtca accggcgaca
    16621 ttgatcccgg aagcccagcc tgcgccgcat caatggcaga aaatgccatt ctggcagaaa
    16681 gtacgtccgt gggcggcaca gcttacccaa atgggcgtag ccgcatgcgt atcgcttgca
    16741 gttatcgttg gcgtccagca ctataatgga caatctgaaa cgtcccagca gcccgaaacg
    16801 ccggtattta atacactgcc gatgatgggt aaagccagcc cggtaagcct gggagtacct
    16861 tctgaagcga ccgcaaacaa tggtcaacag cagcaggtac aggagcagcg tcgtcgcatt
    16921 aatgcaatgt tgcaggatta cgaactgcaa cgccgactcc actctgaaca gcttcagttt
    16981 gagcaggcgc aaactcagca agccgctgta caggtgccag gaattcaaac tttaggaacg
    17041 caatcgcagt aatgaagcaa ctttggtttg ccatgtcatt agtgacaggt agcctgttat
    17101 tctctgctaa cgcctcggcc actcccgcgt ccggggcgtt attacagcag atgaacctgg
    17161 ccagtcagtc actgaattac gagctgtcat tcatcagcat caataaacag ggtgttgagt
    17221 ctctgcgtta tcgacatgca cgcctcgata accgtcctct tgcacaattg ttgcaaatgg
    17281 atggcccgcg ccgggaagtg gtacagcgcg gcaatgaaat cagctatttt gaaccgggac
    17341 ttgaaccgtt cacgcttaat ggcgattaca ttgttgattc tctaccatcg ctcatctata
    17401 ccgatttcaa acgcctttct ccttactacg actttatctc cgtcgggcgc acgcgtattg
    17461 ctgatcgtct ttgcgaagtc attcgtgtgg ttgcccgaga tggtacacgc tacagctaca
    17521 tcgtgtggat ggacaccgaa tcgaaattac cgatgcgggt tgatctcctt gatcgcgatg
    17581 gtgaaacgct ggaacaattt cgcgtgattg cttttaatgt caatcaggat atcagcagca
    17641 gtatgcagac gctggcgaag gcaaatttgc cgccgttgct ttctgttcct gtaggtgaaa
    17701 aagctaaatt cagctggacg ccaacctggt tgccacaggg ttttagcgaa gtttccagta
    17761 gtcgacgtcc gctaccgacg atggacaaca tgcctatcga atcacgtctc tattccgacg
    17821 gattattcag cttctcggta aacgttaacc gcgctacgcc atcgagcacc gatcagatgt
    17881 tgcgcaccgg gcgcagaacc gtcagtacaa gcgtacgtga taacgccgaa atcaccattg
    17941 tcggtgaact gccgccgcaa acggcgaaac gcattgccga gaatattaag ttcggggcag
    18001 cgcaatgatc aaagagtggg ctaccgtcgt ctcctgggaa aacgggcagg cgctggttag
    18061 ttgtgatgtt aaagcctcat gcagcagctg cgcttcacgt gccggttgcg gtagccgcgt
    18121 gttgaataaa cttggcccgc aaaccacgca taccattgtc gtaccctgtg atgagccgtt
    18181 agtgccgggg caaaaagtag aattaggaat cgccgaaggc agcctgctta gctccgcatt
    18241 actggtttat atgtcgccgt tggtgggact attcctcatc gcttcactat ttcagctact
    18301 ctttgcttca gatattgcag cattatgcgg tgcgattctc ggtggcgttg gtggattcct
    18361 gattgcacgt ggctgctctc gaaagtttgc tgcccggtct gaatggcaac cgatcatctt
    18421 aagcgtggcc ttgccgcctg gccttgtgcg atttgaaaca tcttcggaag acgcgagcca
    18481 gtgatttcac tggctttgtt gcattttgca taacaaatgc gaggacgttt ccagatacca
    18541 caatccatac ggtatgaaca tttcctcgcc tcaatgttgt agtgtagaat gcggcgtttc
    18601 tattaataca gacgttaagc tcagaacagc gacttctaaa agcctggtta accgggcatt
    18661 aaggcacaat aatcatactt tatgaagaat atacgtaact tttcgatcat agctcacatt
    18721 gaccacggta aatcgacgct gtctgaccgt attatccaga tctgcggtgg cctgtctgac
    18781 cgtgaaatgg aggcgcaggt tctcgattcc atggatcttg agcgtgagcg tggcattacc
    18841 atcaaagcgc aaagcgtgac gctggactac aaagcgtctg acggcgaaac ctatcagctt
    18901 aactttatcg acaccccggg ccacgtagac ttctcctatg aagtttctcg ttcgctggct
    18961 gcctgtgaag gtgcattgct ggtggtcgac gccgggcagg gcgtagaagc gcaaaccctg
    19021 gcaaactgct acaccgccat ggaaatggat ctcgaagtgg tgccggtact gaacaagatt
    19081 gacctgccgg cagccgatcc tgaacgcgtg gcggaagaaa ttgaagatat cgtcggcatc
    19141 gacgccaccg acgcggtgcg ctgttcagcg aaaaccggcg ttggcgtaca ggacgttctc
    19201 gaacgtctgg tgcgcgacat tccgccgccg gaaggcgatc cggaaggccc gttgcaggca
    19261 ctcattatcg actcctggtt cgacaactac ctgggcgttg tttcacttat ccgtattaaa
    19321 aacggcaccc tgcgtaaggg cgacaaagtg aaagtcatga gtaccgggca gacctataac
    19381 gccgaccgtc tgggcatctt cacgccgaag caggttgacc gtactgaact gaaatgcggc
    19441 gaagtaggct ggctggtatg tgcgattaaa gatatccacg gcgctccagt aggcgatacc
    19501 ttaacgctgg cgcgtaatcc ggcagaaaag gcgctgcctg gctttaagaa agtcaaaccg
    19561 caggtatacg ccggtctgtt cccggtaagt tccgacgact atgaagcctt ccgtgacgcg
    19621 ctgggcaaac tcagcctgaa cgatgcttca ctgttctatg agccggaaag ctccagcgcg
    19681 ctgggctttg gtttccgctg cggcttcctc ggcctgctgc acatggagat catccaggaa
    19741 cgtctggaac gtgaatacga tctggatctg atcaccactg cgccgaccgt agtgtatgaa
    19801 gttgaaacca cgtcaagaga agttatctac gtcgacagcc catccaagct gccagcggta
    19861 aataacattt acgaactgcg cgagccgatt gcagagtgtc acatgctgct gccgcaggca
    19921 tatctcggca acgttattac gctgtgcgta gaaaaacgcg gcgtgcagac caatatggtt
    19981 taccacggta atcaggtggc gctgacgtac gagatcccga tggcggaagt ggtgctcgac
    20041 ttcttcgatc gcctgaaatc tacctcgcgt ggttatgcgt ctctggatta caacttcaaa
    20101 cgcttccagg cgtccgacat ggtacgtgta gacgtattaa tcaacggtga acgtgttgat
    20161 gcgctggcgt tgatcaccca ccgtgataat tcgcaaaacc gcggtcgcga gctggtggag
    20221 aagatgaaag atctgatccc acgccagcag tttgatatcg ccattcaggc agcgattggt
    20281 acgcacatca ttgcgcgctc caccgtgaaa cagctgcgta aaaacgtact ggctaaatgt
    20341 tatggcggcg atatcagccg taagaaaaag ctgctgcaga agcagaaaga aggtaagaaa
    20401 cgcatgaagc agatcggtaa cgtcgagctg ccgcaggaag cgttcctcgc cattctgcac
    20461 gtcggcaaag acaacaaata acccttagga gttggcatgg cgaatatgtt tgccctgatt
    20521 ctggtgattg ccacactggt gacgggcatt ttatggtgcg tggataaatt ctttttcgca
    20581 cctaaacggc gggaacgtca ggcagcggcg caggcggctg ccggtgactc actggataaa
    20641 gcaacgctga agaaagttgc gccgaagcct ggctggctgg aaaccggtgc ttctgttttt
    20701 ccggtgctgg ctatcgtatt gattgtgcgt tcgtttattt atgaaccgtt ccagatcccg
    20761 tcaggttcga tgatgccgac tctgttaatc ggtgatttta ttctggtaga gaagtttgct
    20821 tatggcatta aagatcctat ctaccagaaa acgctgatcg aaaccggtca tccgaaacgc
    20881 ggcgatatcg tggtctttaa atatccggaa gatccaaagc ttgattacat caagcgcgcg
    20941 gtgggtttgc cgggcgataa agtcacttat gatccggtct caaaagagct gacgattcaa
    21001 ccgggatgca gttccggcca ggcgtgcgaa aacgcgctac cggtcactta ctccaacgtg
    21061 gaaccgagcg atttcgttca gaccttctca cgccgtaatg gtggggaagc gaccagcgga
    21121 ttctttgaag tgccgaaaaa cgaaaccaaa gaaaatggaa ttcgtctttc cgagcgtaaa
    21181 gagacattgg gtgatgtgac gcaccgcatt ctgacagtgc cgattgcgca ggaccaggtg
    21241 gggatgtatt accagcagcc agggcaacaa ctggcaacct ggattgttcc gccgggacaa
    21301 tacttcatga tgggcgacaa ccgcgacaac agcgcggaca gccgttactg gggctttgtg
    21361 cctgaagcga atctggtcgg tcgggcaacg gctatctgga tgagcttcga taagcaagaa
    21421 ggcgaatggc cgactggtgt gcgcttaagt cgcattggcg gtatccatta atagccatct
    21481 tcgttcacgt tgtcgccgtt atggcgacaa cgtgaattat ttatgagata aatctcccgt
    21541 ggctaacgac atcccccgtc gttgtgtata gaatattccc ccgaagttta aggttggcac
    21601 ctccaggttg ccacggcaca cgaaacagcg ttggtcccca tataccggta aactgaaact
    21661 gcagtgaagc agttagccga gccatgtata tcaggtctgt ttcgtgtgct gaattgttga
    21721 cgcatttatt tattggtatc gcatgaaccc catcgtaatt aatcggcttc aacggaagct
    21781 gggctacact tttaatcatc aggaactgtt gcagcaggca ttaactcatc gtagtgccag
    21841 cagtaaacat aacgagcgtt tagaattttt aggtgactct attctgagct acgttatcgc
    21901 caatgcgctt tatcaccgtt tccctcgtgt ggatgaaggc gatatgagcc ggatgcgcgc
    21961 cacgctggtc cgtggtaata cgctggctga actggcgcgc gaatttgagt taggtgagtg
    22021 cttacgttta gggccaggtg aacttaaaag cggtggattt cgtcgtgagt caattctcgc
    22081 tgacaccgtc gaggcattaa ttggtggcgt attcctcgac agtgatattc aaaccgtcga
    22141 gaaattaatc ctcaactggt atcaaactcg tctggacgaa attagcccag gcgataaaca
    22201 aaaagatccg aaaacgcgtc tgcaggaata tttgcagggt cgccatctgc cgctgccaac
    22261 ttatctggta gtccaggtac gtggcgaagc gcacgatcag gaatttacta tccactgcca
    22321 ggtcagcggc ctgagtgaac cggtggttgg cacaggttca agccgtcgta aggctgagca
    22381 ggctgccgcc gaacaggcgt tgaaaaaact ggagctggaa tgagcatcga taaaagttac
    22441 tgcggattta ttgctatcgt cggacgtccg aacgttggca aatccacatt gttgaacaaa
    22501 ctgctggggc agaagatctc catcacctcc cgcaaggcgc aaacaactcg tcaccgcatt
    22561 gtggggatcc atactgaagg cgcgtatcag gcgatctacg ttgatacccc aggcctgcat
    22621 atggaagaaa aacgcgccat taaccgcctg atgaacaaag cggcgagcag ctctatcggc
    22681 gacgttgagc tggtgatttt tgtcgtagaa ggcacccgct ggacgccgga cgatgaaatg
    22741 gtgctcaaca aactgcgcga tggcaaagcg ccggtaatcc tcgcggtgaa caaagtggat
    22801 aacgtgcagg aaaaagccga tctgctgccg cacttacagt tcctggcaag ccagatgaac
    22861 ttcctggata ttgtgccgat ctctgccgaa accgggctga atgtcgacac tatcgcagca
    22921 atcgtgcgta agcatctgcc tgaagcgacc catcacttcc cggaagatta catcaccgat
    22981 cgctcacagc gttttatggc gtctgaaatc atccgcgaaa aactgatgcg tttcctcggc
    23041 gctgaactgc cgtactccgt gaccgtggag atcgaacgtt tcgtctctaa cgaacgcggc
    23101 ggttatgaca tcaacggttt gattctcgtt gagcgtgaag ggcagaagaa gatggttatt
    23161 ggcaacaaag gggccaagat caaaaccatc gggattgaag cgcgtaaaga catgcaggaa
    23221 atgtttgaag cgcctgttca ccttgagctg tgggtaaaag tgaaatccgg ttgggccgac
    23281 gacgaacgcg cactgcgcag tctcggttat gttgacgatc tttaagagta actccgatgg
    23341 aaggctggca gcgcgcattt gtcctgcata gtcgcccgtg gagcgaaacc agcctgatgc
    23401 tggacgtctt cacggaggaa tcgggtcgcg tgcgtctggt tgccaaaggc gcacgctcta
    23461 aacgctctac tctgaaaggt gcattacaac ctttcacccc tctcttgctt cgcttcggtg
    23521 ggcgtggtga agtcaaaaca ctgcgcagtg ctgaagctgt ctcgctggcg ctgccattaa
    23581 gcggtatcac gctttacagc ggtctgtaca tcaacgaact tctctcccgc gtactggaat
    23641 acgagacgcg cttctctgaa ctctttttcg attacttgca ctgcattcag tcacttgcag
    23701 gtgccactgg tacgccagaa cccgcgctgc gtcgctttga actggcactg ctcgggcatc
    23761 tgggctatgg cgtcaatttt acccattgtg cgggtagcgg cgagtcggta gatgacacca
    23821 tgacgtatcg ttatcgcgaa gaaaaagggt ttatcgcaag cgtcgttatc gacaataaaa
    23881 cgttcaccgg aaggcagtta aaagcgttaa acgcgcggga atttcctgac gcagacacac
    23941 tgcgcgccgc gaaacgcttt acccgcatgg cgcttaagcc gtatcttggc ggtaaaccat
    24001 taaagagcag ggaactgttc cggcagttta tgcctaagcg aacggtgaaa acacattatg
    24061 aatgatgagg attgtcatgg ctgaattact gttaggcgtc aacattgacc atattgcaac
    24121 gctgcgtaac gcacgcggta ctgcttaccc ggatccggtg caggcggcgt ttattgctga
    24181 gcaggcggga gcggacggca ttaccgtgca tttacgtgaa gatcgccgcc acattactga
    24241 ccgcgatgtg cgcatcctgc gtcagacgct ggatacccgc atgaatctgg agatggcggt
    24301 gaccgaagag atgctggcga tcgccgttga gacgaagcca catttttgct gtctggtacc
    24361 ggaaaagcgt caggaagtaa caaccgaagg cggcctggac gtcgcagggc agcgtgacaa
    24421 aatgcgcgat gcctgtaaac gtctggcgga ggccgggatc ccggtttctc tgtttatcga
    24481 cgccgatgaa gagcagatca aagctgcggc agaggttggc gcaccgttta tcgagatcca
    24541 caccggttgc tatgctgatg ccaaaactga cgccgaacag gcgcaagagc tggcgcgtat
    24601 cgccaaagcc gcgacctttg ccgctagtct cggtctgaaa gttaacgctg gtcacggtct
    24661 gacctatcac aacgtgaaag ctattgccgc catccctgag atgcatgaac tgaatatcgg
    24721 tcatgccatt attggtcgtg cagtgatgac cggactgaaa gatgcggtgg cagaaatgaa
    24781 gcgtctgatg ctggaagcgc gtggctaatg gcaatattag gtttaggtac ggatattgtg
    24841 gagatcgctc gcatagaagc ggtgatcgcc cgatccggtg agcgcctggc acgccgcgta
    24901 ttaagcgata acgaatgggc aatctggaaa acgcaccacc agccggtgcg ttttctggcg
    24961 aagcgttttg ctgtgaaaga agcggcggca aaagcgtttg gcaccgggat ccgcaatggt
    25021 ctggcgttta atcaatttga agtattcaat gatgagctcg gcaaaccacg gctacggcta
    25081 tggggtgagg cattaaaact ggcggaaaag ctcggcgttg taaatatgca tgtaacgctg
    25141 gcagatgaac gccactatgc ttgtgccacg gtaattattg aaagttaaaa aaataccgtt
    25201 ctctttataa agtaaaaccc ccgaactggt aaggaacggg ggttttcact ttacaacttt
    25261 aattttgtgc agcacacgat tccaacagaa atgattatgg atcaatttcg ttatcctgta
    25321 caagcaactt gcaatctctg acataaaaaa cgcgagcctt tacgaagaaa tcgacattga
    25381 taaaaagatt aacgtgaccg ctttttgata agtacattaa aatcggtttc gttattggca
    25441 attttgctgt gctttacacc atgccactga attcccccat tgaaacgagt ggtgtcgtca
    25501 aagctctggt gtggagtgca gcactcgatt ccaataactc gcacgttcag ttttggggtg
    25561 atgtaagggc taatctgaac ggctgcattc cttgtttaag gaaaaacgaa tgactgattg
    25621 ccgatacctg attaaacgga ttatcaaaat catcattgct gttttacagc tgatccttct
    25681 gttcttataa cacaaggaaa cgtacttaag gtgcgtccgg tgaaccagtc ggacgcgcct
    25741 ttaataacta taaa
//
LOCUS       DAZFLD010000056        25633 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.056, whole genome shotgun sequence.
ACCESSION   DAZFLD010000056 DAZFLD010000000
VERSION     DAZFLD010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25633
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.056"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(200..1012)
                     /locus_tag="ACHKBL_001913"
     CDS             complement(200..1012)
                     /locus_tag="ACHKBL_001913"
                     /EC_number="3.-.-.-"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001343535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HFO5818792.1"
                     /translation="MATTQQSGFAPAASPLASTIVQTPDDAIVAGFTSIPSQGDNMPA
                     YHARPKQSDGPLPVVIVVQEIFGVHEHIRDICRRLALEGYLAIAPELYFREGDPNDFA
                     DIPTLLSGLVAKVPDSQVLADLDHVASWASRNGGDVHRLMITGFCWGGRITWLYAAHN
                     PQLKAAVAWYGKLTGDKSLNSPKQPVDIATDLNAPVLGLYGGQDNSIPQESVETMRQA
                     LRAANAKAEIIVYPDAGHAFNADYRPSYHAESAKDGWQRMLEWFKQYGGKRN"
     gene            1274..2038
                     /gene="udp"
                     /locus_tag="ACHKBL_001914"
     CDS             1274..2038
                     /gene="udp"
                     /locus_tag="ACHKBL_001914"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001593007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HFO5818793.1"
                     /translation="MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKL
                     ASHREFTTWRAELDGKPVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHI
                     NVGDVLVTTASVRLDGASLHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTASSD
                     TFYPGQERYDTYSGRVVRHFKGSMEEWQAMGVMNYEMESATLLTMCASQGLRAGMVAG
                     VIVNRTQQEIPNAETMKQTESHAVKIVVEAARRLLK"
     gene            complement(2072..3562)
                     /locus_tag="ACHKBL_001915"
     CDS             complement(2072..3562)
                     /locus_tag="ACHKBL_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001606539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HFO5818794.1"
                     /translation="MFESELLTQGFSTLLNNPQALLFATFGVMLGIVIGALPGLTATM
                     GVAILLPFTYGMEPVSGLLMICGVFFGGVYGGSITAILLKIPGTPAAAATAIDGYELT
                     KQGKAGLALSAATFSSFSGGTLSIIVLMFLSPVLASWALKFSASESFALATFGLSIIA
                     SISGESLIKGLIAGVGGLLIATIGLDPMGGFPRFTGGFVELMNVPFIPVMIGLFAASE
                     AFRSMEQNQQIRQGAKVAIGSLLLPWQTLRRIALTILRSSGLGVFIGMIPGAGADIAA
                     FVAYNETRRFSKTPENFGKGEIKAVASCEAGANGCTGGALLPMLTLGIPGDAVTAIML
                     GALTLQGMQPGPLMFTDHGDMVYTLFVGMIFCYFMLLVLGLLSLKVIGNVVKIPGNIL
                     TPMILALCVVGTYALNNSLFDVGIMLIAGVVGYFMQKGGYPASPVVLALIMGPMAESN
                     FRRALSLSGGSLDFLYTRPITLALLTLAAFTLLTPIIRKIMRLRRQ"
     gene            complement(3575..4081)
                     /locus_tag="ACHKBL_001916"
     CDS             complement(3575..4081)
                     /locus_tag="ACHKBL_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001596577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HFO5818795.1"
                     /translation="MNTKQSVAQLAVPHRKRLSSTMVVALLLCVVAGAVMINAADFPA
                     TAIETDPGASAFPTFYACALIVLAVLLVIRDLLQAKPASCANAQEKPAFRKTATGIAA
                     TAFYIVAMSYCGYLITTPVFLIVIMTLMGYRRWVLTPGIALLLTAILWLLFVEALQVP
                     LPVGTFFE"
     gene            complement(4098..5072)
                     /locus_tag="ACHKBL_001917"
     CDS             complement(4098..5072)
                     /locus_tag="ACHKBL_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HFO5818796.1"
                     /translation="MIKHLTLPKKMAAMVLLAGAALSIAPVQAASYPTKQIELVVPYA
                     AGGGTDLVARAFADAAKNHLPVSIGVINKPGGGGAIGLSEIAAARPNGYKIGLGTVEL
                     TTLPSLGMVRFKTSDFKPIARLNADPAAITVRADAPWNSYEEFMAYSKANPGKVRIGN
                     SGTGAIWHLAVAALEDKTGTKFSHVPYDGAAPAITGLLGGHIEAVSVSPGEVINHVNG
                     GKLKTLVVMADERMKTMPDVPTLKEKGVDLSIGTWRGLIVSQKTPQDVVDVLAKAAKE
                     TAEEPAFQDALQKLNLNYAWLDAASFQTQISEQEKYFDELLTRLGLKK"
     gene            complement(5097..5726)
                     /locus_tag="ACHKBL_001918"
     CDS             complement(5097..5726)
                     /locus_tag="ACHKBL_001918"
                     /EC_number="4.1.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001116470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008674 -
                     2-dehydro-3-deoxy-6-phosphogalactonate aldolase activity
                     [Evidence IEA];
                     GO_process: GO:0034194 - D-galactonate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HFO5818797.1"
                     /translation="MPFNTLLQKTGLVAILRGVKPDEIVAIGEKLYAAGFRLIEIPMN
                     SPEALQSISLLRDALPKDCLVGAGTVLSVEQVVAVKEAGGQIIVMPHCDTAVIRRARA
                     LGMYCAPGVATPTEAFAAIEHGANAIKLFPAEQITPEVTKAWRAVIPQSVPMLPVGGI
                     TPETMARYLSHGANGFGLGSALYRPGMTPEQVYENAVLFMNAWNNLNSN"
     gene            complement(5716..6720)
                     /locus_tag="ACHKBL_001919"
     CDS             complement(5716..6720)
                     /locus_tag="ACHKBL_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HFO5818798.1"
                     /translation="MPGAPSFIALDWGTSSLRAWRFGESPNPQEKREFPWGIMKLPSQ
                     AATREDAFHDTFLRVCGDWLAQTPCPVLACGMLGSAQGWQSAAYLPCPVTLEGLAKQL
                     TPVIHQQQTMLHIIPGVIKEGEMPEVMRGEETQIFGAISMEPALQNAIHQGMPVLIGL
                     PGTHAKWAVVENNTITDFRTFMTGELFDVLSRHSILGATMHPGDEPHWDAFTHGLTAA
                     QEHHQTGLLSTLFSTRSRLLTSNLTSSSQGDYLSGLLIGHELCGLASSLLRDLPATTP
                     IALIGSANLNSRYSQAFSHVFPDRQIHAIPNATEHGLWRIAHAAGLLSTNARECTHAI
                     "
     gene            complement(6744..7511)
                     /locus_tag="ACHKBL_001920"
     CDS             complement(6744..7511)
                     /locus_tag="ACHKBL_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HFO5818799.1"
                     /translation="MAETSSTTAGAQTLLRGLAVLNAVYNGCHDLKSIGEFTGTTRST
                     THRLVTVLVEQRYLRHVPTQGYQLGAKLIEFGARALESTSLYEIAQPVLQRLARYTLD
                     TVHLGIVEGDEVLYLEKINSQRGLEMRSRPGHRMPLAITGIGKALILNRTEEEWRTLF
                     KTCGDETKLGAFIQNMRRYAASGFAFDLEENEPTIRCVAAPVYNARDEIVAAISVAST
                     TTYMSLARLEELAPYVKSCAEEISAELGWGKHVRKDK"
     gene            7728..9155
                     /gene="rmuC"
                     /locus_tag="ACHKBL_001921"
     CDS             7728..9155
                     /gene="rmuC"
                     /locus_tag="ACHKBL_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HFO5818800.1"
                     /translation="MDFSIIVYAIIALVSVVIGWLFASYQHAQQKAEQLAEREEMVAE
                     LSAAKQQITQSEHWRAECELLNNEVRSLQSINTSLEADLREVTTRMEAAQQHADDKIR
                     QMINSEQRLSEQFENLANRIFEHSNRRVDEQNRQSLNSLLSPLREQLDGFRRQVQDSF
                     GKEAQERHTLTHEIRNLQQLNAQMAQEAINLTRALKGDNKTQGNWGEVVLTRVLEASG
                     LREGYEYETQVSIENDARSRMQPDVIVRLPQGKDVVIDAKMTLVAYERYFNAEDDYTR
                     ESALQEHIASVRNHIRLLGRKDYQQLPGLRTLDYVLMFIPVEPAFLLALDRQPELITE
                     ALKNNIMLVSPTTLLVALRTIANLWRYEHQSRNAQQIADRASKLYDKMRLFVDDMSAI
                     GQSLDKAQDNYRQAMKKLSSGRGNVLAQAEAFRGLGVEIKREINPDLAEQAVSQDEEY
                     RLRSVPEQPNDEAYQRDDEYNQQSR"
     gene            9250..10005
                     /gene="ubiE"
                     /locus_tag="ACHKBL_001922"
     CDS             9250..10005
                     /gene="ubiE"
                     /locus_tag="ACHKBL_001922"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HFO5818801.1"
                     /translation="MVDKSQETTHFGFQTVAKEQKADMVAHVFHSVASKYDVMNDLMS
                     FGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGETGKVVLADINESMLK
                     MGREKLRNIGVIGNVEYVQANAEALPFPDNTFDCITISFGLRNVTDKDKALRSMYRVL
                     KPGGRLLVLEFSKPIIEPLSKAYDAYSFHVLPRIGSLVANDADSYRYLAESIRMHPDQ
                     DTLKTMMQDAGFESVDYYNLTAGVVALHRGYKF"
     gene            10019..10624
                     /gene="ubiJ"
                     /locus_tag="ACHKBL_001923"
     CDS             10019..10624
                     /gene="ubiJ"
                     /locus_tag="ACHKBL_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiJ"
                     /protein_id="HFO5818802.1"
                     /translation="MPFKPLVTAGIESLLNTFLYRSPALKTARSRLLGKVLRVEVKGF
                     STSLILVFSERQVDVLGEWAGDADCTVIAYASVLPKLRDRQQLAALIRSGELEVQGDI
                     QVVQNFVALADLAEFDPAELLAPYTGDIAAEGISKALRGGAKFLHHGIKRQQRYVAEA
                     ITEEWRMAPGPLEVAWFAEETAAVERAVDALTKRLEKLEAK"
     gene            10621..12261
                     /gene="ubiB"
                     /locus_tag="ACHKBL_001924"
     CDS             10621..12261
                     /gene="ubiB"
                     /locus_tag="ACHKBL_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HFO5818803.1"
                     /translation="MTPGEVRRLYFIIRTFLSYGLDELIPKMRITLPLRLWRYSLFWM
                     PNRHKDKPLGERLRLALQELGPVWIKFGQMLSTRRDLFPPHIADQLALLQDKVAPFDG
                     KLAKQQIEAAMGGLPVEAWFDDFEIKPLASASIAQVHTARLKSNGKEVVIKVIRPDIL
                     PVIKADLKLIYRLARWVPRLLPDGRRLRPTEVVREYEKTLIDELNLLRESANAIQLRR
                     NFEDSPMLYIPEVYPDYCSEGMMVMERIYGIPVSDVATLEKNGTNMKLLAERGVQVFF
                     TQVFRDSFFHADMHPGNIFVSYEHPENPKYIGIDCGIVGSLNKEDKRYLAENFIAFFN
                     RDYRKVAELHVDSGWVPPDTNVEEFEFAIRTVCEPIFEKPLAEISFGHVLLNLFNTAR
                     RFNMEVQPQLVLLQKTLLYVEGVGRQLYPQLDLWKTAKPFLESWIKDQVGIPALVRAF
                     KEKAPFWVEKMPELPELVYDSLRQGKYLQHSVDKIARELQSNHVRQGQSRYFLGIGAT
                     LVLSGTFLLVSRPEWGLMPGWLMAGGLIAWFVGWRKTR"
     gene            12340..12609
                     /gene="tatA"
                     /locus_tag="ACHKBL_001925"
     CDS             12340..12609
                     /gene="tatA"
                     /locus_tag="ACHKBL_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HFO5818804.1"
                     /translation="MGGISIWQLLIIAVIVVLLFGTKKLGSIGSDLGASIKGFKKAMS
                     DDEPKQDKTSQDADFTAKTIADKQADTNQEQAKTEDAKRHDKEQV"
     gene            12613..13128
                     /gene="tatB"
                     /locus_tag="ACHKBL_001926"
     CDS             12613..13128
                     /gene="tatB"
                     /locus_tag="ACHKBL_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HFO5818805.1"
                     /translation="MFDIGFSELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRSLAT
                     TVQNELTQELKLQEFQDSLKKVEKASLTNLTPELKASMDELRQAAESMKRSYVANDPE
                     KASDEAHTIHNPVVKDNEAAHEGVTPAAAQTQASSPEQKPETTPEPVVKPAADAEPKT
                     AAPSPSSSDKP"
     gene            13131..13907
                     /gene="tatC"
                     /locus_tag="ACHKBL_001927"
     CDS             13131..13907
                     /gene="tatC"
                     /locus_tag="ACHKBL_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatC"
                     /protein_id="HFO5818806.1"
                     /translation="MSVEDTQPLITHLIELRKRLLNCIIAVIVIFLCLVYFANDIYHL
                     VSAPLIKQLPQGSTMIATDVASPFFTPIKLTFMVSLILSAPVILYQVWAFIAPALYKH
                     ERRLVVPLLVSSSLLFYIGMAFAYFVVFPLAFGFLANTAPEGVQVSTDIASYLSFVMA
                     LFMAFGVSFEVPVAIVLLCWMGITSPEDLRKKRPYVLVGAFVVGMLLTPPDVFSQTLL
                     AIPMYCLFEIGVFFSRFYVGKGRNREEENDAEAESEKTEE"
     gene            13949..14731
                     /gene="tatD"
                     /locus_tag="ACHKBL_001928"
     CDS             13949..14731
                     /gene="tatD"
                     /locus_tag="ACHKBL_001928"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' ssDNA/RNA exonuclease TatD"
                     /protein_id="HFO5818807.1"
                     /translation="MFDIGVNLTSSQFAKDRDDVVARAFDAGVNGLLITGTNLRESQQ
                     AQKLARQYSSCWSTAGVHPHDSSQWQAATEEAIIELAAQPEVVAIGECGLDFNRNFST
                     PEEQERAFVAQLRIAAELNMPVFMHCRDAHERFMTLLEPWLDKVPGAVLHCFTGTREE
                     MQACVAHGIYIGITGWVCDERRGLELRELLPLIPAEKLLIETDAPYLLPRDLTPKPSS
                     RRNEPAHLPHILQRIAHWRGEDAAWLAATTDANVKTLFGIAF"
     gene            complement(14728..15216)
                     /gene="rfaH"
                     /locus_tag="ACHKBL_001929"
     CDS             complement(14728..15216)
                     /gene="rfaH"
                     /locus_tag="ACHKBL_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="HFO5818808.1"
                     /translation="MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKIVRGKRT
                     AVSEPLFPNYLFVEFDPEVIHTTTINATRGVSHFVRFGASPAIVPSAVIHQLSVYKPK
                     DIVDPSTPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
                     KL"
     gene            15383..16876
                     /gene="ubiD"
                     /locus_tag="ACHKBL_001930"
     CDS             15383..16876
                     /gene="ubiD"
                     /locus_tag="ACHKBL_001930"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HFO5818809.1"
                     /translation="MDAMKYNDLRDFLTLLEQQGELKRITLPVDPHLEITEIADRTLR
                     AGGPALLFENPKGYSMPVLCNLFGTPKRVAMGMGQEDVSALREVGKLLAFLKEPEPPK
                     GFRDLFDKLPQFKQVLNMPTKRLRGAPCQQKIVSGDDVDLNRIPIMTCWPEDAAPLIT
                     WGLTVTRGPHKERQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEWCAAHPGERFPVS
                     VALGADPATILGAVTPVPDTLSEYAFAGLLRGTKTEVVKCISNDLEVPASAEIVLEGY
                     IEQGETAPEGPYGDHTGYYNEVDSFPVFTVTHITQREDAIYHSTYTGRPPDEPAVLGV
                     ALNEVFVPILQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYAGHAKRVMMGVWSFLRQF
                     MYTKFVIVCDDDVNARDWNDVIWAITTRMDPARDTVLVENTPIDYLDFASPVSGLGSK
                     MGLDATNKWPGETQREWGRPIKKDPDVVAHIDAIWDELAIFNNGKSA"
     gene            16922..17623
                     /gene="fre"
                     /locus_tag="ACHKBL_001931"
     CDS             16922..17623
                     /gene="fre"
                     /locus_tag="ACHKBL_001931"
                     /EC_number="1.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HFO5818810.1"
                     /translation="MTTLSCKVTSVEAITDTVYRVRIVPDAAFSFRAGQYLMVVMDER
                     DKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDHQIVVDIPHGEAWLRDDE
                     ERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEQHLYDLCELEALSLKHP
                     GLQVVPVVEQPEAGWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSER
                     NAREDRLFGDAFAFI"
     gene            complement(17815..18978)
                     /gene="fadA"
                     /locus_tag="ACHKBL_001932"
     CDS             complement(17815..18978)
                     /gene="fadA"
                     /locus_tag="ACHKBL_001932"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003988 - acetyl-CoA C-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadA"
                     /protein_id="HFO5818811.1"
                     /translation="MEQVVIVDAIRTPMGRSKGGAFRHVRAEDLSAHLMRSLLARNPA
                     LEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAA
                     RMIMIGDAQTCLVGGVEHMGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGI
                     SREMQDAFAARSHARAWAATQSGAFKNEIIPTGGHDADGVLTQFNYDEVIRPETTVEA
                     LATLRPAFDPVSGTVTAGTSSALSDGAAAMLVMSESRAHELGLKPRARVRSMAVVGCD
                     PSIMGYGPVPASKLALKKAGLSVSDIGVFEMNEAFAAQILPCIKDLGLIEQIDEKINL
                     NGGAIALGHPLGCSGARISSTLLNLMERKDAQFGLATMCIGLGQGIATVFERI"
     gene            complement(18988..21177)
                     /gene="fadB"
                     /locus_tag="ACHKBL_001933"
     CDS             complement(18988..21177)
                     /gene="fadB"
                     /locus_tag="ACHKBL_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0036125 - fatty acid beta-oxidation
                     multienzyme complex [Evidence IEA];
                     GO_function: GO:0003857 - 3-hydroxyacyl-CoA dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0004165 - delta(3)-delta(2)-enoyl-CoA
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0004300 - enoyl-CoA hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008692 - 3-hydroxybutyryl-CoA epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009062 - fatty acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HFO5818812.1"
                     /translation="MLYKGDTLYLDWLEDGIAELVFDAPGSVNKLDTATVASLGEAIG
                     VLEQQSDLKGLLLRSNKAAFIVGADITEFLSLFLVPEEQLSQWLHFANSVFNRLEDLP
                     VPTIAAVNGYALGGGCECVLATDYRLATPDLRIGLPETKLGIMPGFGGSVRMPRMLGA
                     DSALEIIAAGKDVGADQALKIGLVDGVVKAEKLVEGAMAILRQAINGDLDWKAKRQPK
                     LEPLKLSKIEATMSFTIAKGMVAQTAGKHYPAPITAVKTIEAAARFGREEALNLENKS
                     FVPLAHTNEARALVGIFLNDQYVKGKAKKLTKDVETPKQAAVLGAGIMGGGIAYQSAW
                     KGVPVVMKDINDKSLTLGMTEAAKLLNKQLERGKIDGLKLAGVISTIHPTLDYSGFDR
                     VDVVVEAVVENPKVKKAVLAETEQKVRPDTVLASNTSTIPISELANALERPENFCGMH
                     FFNPVHRMPLVEIIRGEKSSDETIAKVVAWASKMGKTPIVVNDCPGFFVNRVLFPYFA
                     GFSQLLRDGADFRKIDKVMEKQFGWPMGPAYLLDVVGIDTAHHAQAVMAAGFPQRMQK
                     DYRDAIDALFNANRFGQKNGLGFWRYKEDSKGKPKKEEDAVVDDLLAEVSQPKRDFSE
                     EEIIARMMIPMVNEVVRCLEEGIIATPAEADMALVYGLGFPPFHGGAFRWLDTLGSAK
                     YLDMAQQYQHLGPLYEVPEGLRNKARHNEPYYPPVEPARPVGDLKMA"
     gene            21367..22698
                     /gene="pepQ"
                     /locus_tag="ACHKBL_001934"
     CDS             21367..22698
                     /gene="pepQ"
                     /locus_tag="ACHKBL_001934"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HFO5818813.1"
                     /translation="MESLASLYKNHIATLQERTRDALARFKLDALLIHSGELFNVFLD
                     DHPYPFKVNPQFKAWVPVTQVPNCWLLVDGVNKPKLWFYLPVDYWHNVEPLPNSFWTE
                     DVEVIALPKADGIGSLLPAARGNIGYIGPVPERALQLGIEASNINPKGVIDYLHYYRS
                     FKTEYELACMREAQKMAVNGHRAAEEAFRSGMSEFDINIAYLTATGHRDTDVPYSNIV
                     ALNEHAAVLHYTKLDHQAPEEMRSFLLDAGAEYNGYAADLTRTWSAKSDNDYAQLVKD
                     VNDEQLALIATMKAGVSYVDYHIQFHQRIAKLLRKHQIITDMSEEAMVENDLTGPFMP
                     HGIGHPLGLQVHDVAGFMQDDSGTHLAAPAKYPYLRCTRILQPGMVLTIEPGIYFIES
                     LLAPWREGQFSKHFNWQKIEALKPFGGIRIEDNVVIHENNVENITRDLKLA"
     gene            22698..23312
                     /locus_tag="ACHKBL_001935"
     CDS             22698..23312
                     /locus_tag="ACHKBL_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HFO5818814.1"
                     /translation="MESWLIPAAPVTVVEEIKKSRFITLLAHTDGVEAAKAFVESVRA
                     EHPDARHHCVAWVAGAPDDSQQLGFSDDGEPAGTAGKPMLAQLMGSGVGEITAVVVRY
                     YGGILLGTGGLVKAYGGGVNQALRQLTTQRKTPLTEYTLQCEYSQLTGIEALLGQCDG
                     KIINSDYQAFVLLRVALPAAKVAEFSAKLADFSRGSLQLLAIEE"
     gene            23351..24802
                     /gene="trkH"
                     /locus_tag="ACHKBL_001936"
     CDS             23351..24802
                     /gene="trkH"
                     /locus_tag="ACHKBL_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkH"
                     /protein_id="HFO5818815.1"
                     /translation="MHFRAITRIVGLLVILFSGTMIIPGLVALIYRDGAGRAFTQTFF
                     VALAIGSMLWWPNRKEKGELKSREGFLIVVLFWTVLGSVGALPFIFSESPNLTITDAF
                     FESFSGLTTTGATTLVGLDSLPHAILFYRQMLQWFGGMGIIVLAVAILPILGVGGMQL
                     YRAEMPGPLKDNKMRPRIAETAKTLWLIYVLLTVACALALWFAGMDAFDAIGHSFATI
                     AIGGFSTHDASIGYFDSPTINTIIAIFLLISGCNYGLHFSLLSGRSLKVYWRDPEFRM
                     FIGVQFTLVVICTLVLWFHNVYSSALMTINQAFFQVVSMATTAGFTTDSIARWPLFLP
                     VLLLCSAFIGGCAGSTGGGLKVIRILLLFKQGNRELKRLVHPNAVYSIKLGNRALPER
                     ILEAVWGFFSAYALVFIVSMLAIIATGVDDFSAFASVVATLNNLGPGLGVVADNFTSM
                     NPVAKWILIANMLFGRLEVFTLLVLFTPTFWRE"
     gene            24814..25359
                     /gene="hemG"
                     /locus_tag="ACHKBL_001937"
     CDS             24814..25359
                     /gene="hemG"
                     /locus_tag="ACHKBL_001937"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HFO5818816.1"
                     /translation="MKTLILFSTRDGQTREIASYLASELKELGIQADVANVHRIEEPQ
                     WENYDRVVIGASIRYGHYHSAFQEFVKKHATRLNSMPSAFYSVNLVARKPEKRTPQTN
                     SYARKFLMSSQWRPDRCAVIAGALRYPRYRWYDRFMIKLIMKMSGGETDTRKEVVYTD
                     WEQVANFAREIAHLTDKPTLK"
BASE COUNT         6062 a         6565 c         6910 g         6096 t
ORIGIN      
        1 agaatgggag ttgatggtaa atctctaagg tgtctgccag tgaagagact catttatgac
       61 tatgacttgc cgactttctt aaggtcaaat agcggagtcg tgctctccaa tcaattcata
      121 ctgattattt tttgggggac agagatggca gcaaaatgca ctcgttcact caccatctca
      181 aatatagact aagaggaaac taattccgct tcccaccata ctgcttaaac cactccagca
      241 tacgctgcca gccatctttc gcagattcgg catgatagct cgggcgataa tcggcgttga
      301 atgcatgccc ggcatccggg tagacgataa tctctgcttt cgcattagca gcccgcagcg
      361 cctggcgcat ggtttcaacg ctctcctgcg gaatgctgtt atcctgacca ccatataagc
      421 cgagaaccgg cgcgttaaga tcggttgcga tatcaacagg ttgtttcggt gaattcagcg
      481 acttgtcgcc cgtcagtttg ccgtaccacg ccactgcggc ttttagctgt ggattatgcg
      541 cggcatacag ccaggtgata cgtccacccc agcagaatcc ggtgatcatt aaacgatgaa
      601 catcgccgcc gttgcgggaa gcccaactgg cgacatgatc gagatcggcc agcacctgcg
      661 agtcaggcac ttttgctacc agaccgctaa gcaacgtggg gatatcggca aaatcattcg
      721 gatcgccttc gcggaagtaa agttcaggtg caatagccag atacccctcc agcgccagac
      781 ggcgacaaat gtcgcggata tgttcatgca cgccaaaaat ttcctgcact acaatgacca
      841 ctggcagtgg gccatcgctt tgctttggtc tggcatggta agcaggcatg ttatcccctt
      901 gtgaagggat agaggtgaag cccgccacaa tcgcgtcgtc cggagtctga acgatggtcg
      961 aagcgagagg cgatgccgca ggtgcaaatc cagattgttg tgttgttgcc atggtattct
     1021 ccgtaccctt ataaaaatgt tgcgcaatgt taactatagt cagcatgcaa caaatcacat
     1081 tgcctgaatc ggctcatctt ttatgcagtc ctgcagaatg aagagtgatt tatgtgattt
     1141 gcatcacttt tgatgggtaa atttatgcaa cgcatttgcg tcatggtgat gagtatcacg
     1201 aaaaaatgtt aaacccttcg gtaaagtgtc tttttgcttc ttctgactaa accgattcac
     1261 agaggagttg tatatgtcca agtctgatgt ttttcatctc ggcctcacta aaaacgattt
     1321 acaaggggct acgcttgcca tcgtccctgg cgacccggat cgtgtggaaa agatcgccgc
     1381 gctgatggat aagccggtta agctggcatc tcaccgcgaa ttcaccacct ggcgtgcaga
     1441 gctggatggt aaacctgtta tcgtctgctc taccggtatc ggcggcccgt ctacctctat
     1501 tgctgttgaa gagctggcac agctgggcat tcgcaccttc ctgcgtatcg gtacaacggg
     1561 cgctattcag ccgcatatta atgtgggtga tgtcctggtt accacggcgt ctgttcgtct
     1621 ggacggcgcg agcctgcact tcgcaccgct ggaattcccg gctgtcgctg atttcgaatg
     1681 tacgactgcg ctggttgaag cggcgaagtc aattggtgcc accactcacg ttggtgtgac
     1741 ggcttcttct gataccttct acccgggtca ggaacgttac gatacttact ctggtcgcgt
     1801 agttcgtcac tttaaaggtt ctatggaaga gtggcaggcg atgggcgtaa tgaactatga
     1861 aatggaatct gcaaccctgc tgaccatgtg tgcaagtcag ggcctgcgtg ccggtatggt
     1921 agcgggggtt atcgttaacc gcacccagca agagatcccg aatgctgaga cgatgaaaca
     1981 aaccgagagc catgcggtga aaatcgtggt ggaagcggcg cgtcgtctgc tgaaataaaa
     2041 aacgctcccc aacttcgggg agcgatatca gttactgacg ccgtaaacgc attattttgc
     2101 ggattattgg cgtcagtagc gtaaaggctg ccagagttag caatgccaga gtgatcggtc
     2161 gggtatacag aaagtccagt gaaccccctg aaagcgacag cgcacggcga aaattacttt
     2221 ctgccatcgg gcccataatc aatgccagca ctacgggtga tgccggatat cctcctttct
     2281 gcatgaaata gcccaccacg cctgcaatca gcataatgcc aacatcaaac aggctattgt
     2341 tcaacgcata agtcccgacc acacaaagtg cgaggatcat cggtgtgaga atattgccgg
     2401 ggatcttcac cacgttaccg atgactttca aagagagcag tccaagaact agcagcatga
     2461 agtaacaaaa gatcatgccg acaaacagtg tataaaccat atcgccgtgg tcggtaaaca
     2521 tcagcgggcc tggttgcatt ccctgtaacg ttaacgcgcc cagcatgatg gcggttaccg
     2581 catcgccagg gatccccagc gtcagcatag gtaacaatgc gccaccggtg cagccattcg
     2641 cgcctgcctc acaggaggcc acagctttaa tttcaccctt accaaagttt tctggtgtct
     2701 tactgaaacg acgagtttcg ttataggcaa caaaagctgc aatatccgcc cccgcaccgg
     2761 ggatcatgcc gataaaaacc cctaatcctg atgagcgcaa aatggttaat gcaatgcggc
     2821 gtagtgtttg ccagggcagt aacagactgc cgatagccac cttcgccccc tgacgaattt
     2881 gctggttttg ctccatagag cggaatgctt ctgaagcagc aaataaacca atcatcaccg
     2941 ggataaatgg cacattcatc agctccacaa atccacctgt aaaccgtgga aaaccgccca
     3001 ttggatcaag gcctatcgtt gcgatcagca atccgccaac cccggcaatc agccctttaa
     3061 tcagcgactc accggaaata ctggcaataa tactcagtcc gaaggttgcc agggcgaagg
     3121 actccgaggc actaaatttc agcgcccaac tggcaagtac cggagagaga aacatcagca
     3181 cgataatgct gagcgttccg ccactaaagg aagagaacgt ggcggcactc aatgccagcc
     3241 ctgcttttcc ctgtttcgtc agctcataac catcaatagc ggtggctgcg gcggcaggcg
     3301 ttccgggaat tttgagcaaa attgcggtga tagaaccacc gtagacgcca ccaaaaaaga
     3361 cgccgcaaat catcaacaag ccagaaacag gctccatgcc gtaggtgaaa ggcagcaaaa
     3421 tcgccacacc catggtcgct gtcagaccag gcaaagcgcc aatcacaata cccagcatca
     3481 ccccaaaagt ggcaaacagc agcgcctgag gattattgag taatgtcgaa aaaccctgag
     3541 ttaaaagttc actttcaaac attgctggcc cctgttattc gaaaaatgtg ccgacaggca
     3601 atggcacctg taacgcttcg acaaacagca accagaggat tgccgttaac agcagcgcaa
     3661 tacccggtgt gagtacccat cgcctgtagc ccatcaacgt cataatgacg atgaggaaaa
     3721 caggagtagt aatgagataa ccgcagtagc tcatcgccac aatataaaac gcggttgccg
     3781 caattcctgt tgctgttttc ctgaatgccg gtttttcctg tgcgttggcg caagaggctg
     3841 gttttgcctg caaaagatcg cgtatcacca gcaagacagc gagcacaatc agggcacagg
     3901 catagaaggt agggaaggca cttgcaccgg gatccgtttc aatggcagtt gctggaaaat
     3961 cagcggcatt aatcatcacc gcgccagcaa ccacacaaag taacagcgcc accaccatcg
     4021 ttgatgaaag gcgcttgcgg tgcggtacgg cgagttgagc aacagactgt tttgtgttca
     4081 tcaaccgtac ctcaggttta ttttttcagg cccaggcgag tcagcaactc gtcaaagtac
     4141 ttttcctgtt cgctgatttg ggtctggaag ctggcagcgt caagccatgc atagttgaga
     4201 ttcaactttt gcagtgcatc ctggaatgca ggctcttcag ccgtctcttt tgctgccttt
     4261 gccagaacat ccaccacatc ctgcggcgtt ttttgcgaca caatcaggcc gcgccaggtg
     4321 ccgatggaga gatcaacgcc tttctctttt aacgtcggga cgtcaggcat ggttttcatt
     4381 cgctcatccg ccatcactac cagtgtcttc agcttgccgc cattcacatg gttgataact
     4441 tctcctgggc ttacggaaac cgcttcaata tgcccgccta acaggcctgt aatggcaggg
     4501 gctgcgccgt catacgggac atgagaaaac tttgtgcccg ttttgtcttc cagtgcagct
     4561 accgccagat gccagatagc tccggtgcct gagttaccaa tgcgtacttt tccgggattc
     4621 gctttggagt aagccataaa ttcttcatag ctattccacg gcgcatcggc acggactgtg
     4681 atagcagccg gatccgcatt cagacgggca atgggtttaa agtcgctggt tttaaaacgc
     4741 accattccga ggctgggaag ggtggtcagt tcaaccgtgc ctaaaccaat tttgtaaccg
     4801 ttagggcggg cagcggcgat ttcactcagg ccgatagcac cgcctccgcc aggtttattg
     4861 ataaccccga tgctgacggg taaatggttt ttggcggcat cagcaaaggc acgggcaacc
     4921 agatccgtac cgcctccggc agcgtaggga acgactaact cgatctgttt ggttgggtag
     4981 gaggcagcct gtacaggcgc aatgctgagg gcagcgcctg caagaagtac cattgcagcc
     5041 atttttttcg gtaaggtaag atgttttatc attgctttta tatccttatt tatcagttag
     5101 ttgctattta aattgttcca tgcgttcata aacagtactg cattttcata gacttgctct
     5161 ggcgtcatgc cggggcgata tagcgcagaa ccgaggccaa agccgtttgc gccgtggctc
     5221 agataacgcg ccatggtttc cggtgtgata ccgccaaccg gtaacatcgg aacggattgt
     5281 ggaataaccg cacgccaggc tttggtaact tctggcgtaa tttgttcagc cgggaacagc
     5341 ttgatggcat tagcaccatg ttcaatggca gcaaaggctt cggtgggcgt ggcgacgcca
     5401 ggcgcacaat acatacccag cgcacgggcg cgacggatca ccgcagtatc gcaatgcggc
     5461 atcacgataa tctggccccc agcctcttta accgcaacca cctgctctac cgaaagcaca
     5521 gttccggctc cgaccaggca gtccttaggc agggcgtcgc gcagtaaact gatactttgc
     5581 aacgcctccg gcgagttcat aggaatctca atcaggcgga atccggcggc gtacagtttt
     5641 tcaccgatgg cgacaatctc atccggtttc acgccgcgta aaatggcgac taacccggtt
     5701 ttttgcaaaa gtgtattaaa tggcatgggt acattccctg gcgttggtgg acagtaaccc
     5761 ggcagcgtgg gcgattcgcc ataatccatg ttcagtggca ttaggaatgg cgtgtatctg
     5821 tctgtcggga aaaacgtggc tgaatgcttg tgaatagcgg ctgttcagat ttgcgctacc
     5881 gattaaggcg atcggtgttg tggcgggtaa atcacgtagc aaactggatg ccagaccaca
     5941 taattcatgg cctatcagta atccggaaag gtaatccccc tgcgaggacg acgtaagatt
     6001 actggtcagc aggcgcgaac gggtcgaaaa cagcgtcgat aataatccgg tctgatgatg
     6061 ctcttgtgct gctgtcagcc cgtgggtgaa ggcatcccag tgcggttcat ctccaggatg
     6121 catggtggca ccgagaatcg aatggcggga taaaacatca aataactcgc ccgtcataaa
     6181 ggttcggaaa tcggtaatgg tgttgttttc aactactgcc catttcgcat gtgtgccggg
     6241 taagcctatc agcactggca taccctgatg gatcgcgttt tgcagggccg gttccatcga
     6301 gatagcaccg aagatttgtg tctcttcgcc acgcatcact tcgggcattt caccttcttt
     6361 aatcacgcca ggaatgatgt gcagcattgt ctgctgctgg tggataacag gcgtcaactg
     6421 cttcgccaga ccctcgagag tgaccgggca aggtaaataa gctgctgatt gccacccttg
     6481 cgcactgccg agcatgccgc aagccagtac cgggcatggg gtttgtgcca gccagtcgcc
     6541 gcagacacgt aaaaacgtat cgtggaaagc gtcctcacgt gttgctgcct gggagggtaa
     6601 tttcatgatc ccccagggga attcacgttt ttcttgtgga tttggtgatt cgccaaaacg
     6661 ccatgcccga agcgatgacg ttccccagtc cagggcaata aatgagggtg ctcccggcat
     6721 tagtcgtttc ctttaatcat ggtttattta tctttgcgga cgtgcttacc ccagcccagt
     6781 tcagcggata tctcttctgc acaggatttg acgtaaggtg ccagttcttc cagacgcgcc
     6841 agcgacatat acgtggtggt actggcgaca gaaatagccg ccacaatttc atcccgggcg
     6901 ttgtaaaccg gcgctgcaac gcaacggata gtcggctcgt tctcttccag atcaaaagca
     6961 aaaccactgg cggcataacg acgcatgttc tgaataaatg cgcccagttt tgtttcatca
     7021 ccacaggtct taaacagcgt gcgccactct tcttctgttc ggttgaggat caatgccttg
     7081 ccgatgccgg tgattgccag tggcatgcga tggcctgggc gtgagcgcat ttccagcccg
     7141 cgctgactgt tgatcttctc cagatacagc acttcgtcgc cttcaacgat ccccagatgc
     7201 acggtgtcca gggtgtaacg ggcaaggcgt tgcaaaacgg gctgggcaat ttcgtacagt
     7261 gaggtgcttt ccagtgctct ggcaccgaat tcgatcagtt tggcacccag ttggtagccc
     7321 tgcgttggga catggcgcag gtagcgttgt tccaccagaa cggtgactaa acgatgggtt
     7381 gtgctccggg tcgtgccggt gaactcgccg atacttttta ggtcatggca gccgttataa
     7441 acagcattaa gaaccgccag tccacgtaat aacgtttgtg cgcctgcggt ggttgaagag
     7501 gtttctgcca tacgtttctc ctgatgatat tgctgaagtg ttccacataa tgacgtgggg
     7561 aaaaggtgat atatcccaca atataaactc catctcatat tgtgggataa aataatgaaa
     7621 attaacgatc gttttgcgta gcgcctcgca ggaaacttct ttcaaactgg acgtttgtac
     7681 agcacaattc tattttgtgc gggtaagttg ttgggtcagg aggctttgtg gatttctcaa
     7741 tcattgttta tgccattatt gcgctggtta gtgtggtcat tggctggctg tttgccagtt
     7801 atcaacatgc gcagcaaaaa gccgagcaat tagctgaacg tgaagagatg gtcgcggagt
     7861 taagcgcggc aaaacaacaa attactcaaa gcgagcactg gcgcgctgag tgcgagttac
     7921 tcaataacga agtgcgcagc ctgcaaagta ttaacacctc tctggaggcc gatctgcgtg
     7981 aagtaaccac gcggatggaa gccgcacagc aacatgctga cgataaaatt cgccagatga
     8041 tcaacagcga gcagcgcctc agtgagcagt ttgaaaacct cgccaaccgt atttttgagc
     8101 acagcaatcg ccgggttgat gagcaaaacc gtcagagtct gaacagcctg ttgtcgccgc
     8161 tacgtgaaca actggacggt ttccgccgtc aggttcagga cagcttcggt aaagaagcac
     8221 aggaacgtca taccctgacc cacgaaattc gcaatctcca gcagctcaac gcgcaaatgg
     8281 ctcaggaggc gatcaacctg acgcgcgcgc tgaaaggcga caataaaacc cagggcaact
     8341 ggggcgaggt ggtattgacg cgggtgctgg aggcttccgg tctgcgtgaa gggtatgaat
     8401 atgaaactca ggtcagcatc gaaaatgacg cccgctcgcg gatgcagccg gatgtcatcg
     8461 tgcggttgcc gcagggaaaa gatgtggtga tcgacgccaa aatgacgctg gtcgcctatg
     8521 aacgctattt caacgccgaa gatgactaca cccgcgaaag cgcgctacag gaacatatcg
     8581 cgtcggtgcg taaccatatc cgtttgctgg gacgcaaaga ttatcaacag ctgccggggc
     8641 tgcgaaccct ggattacgtg ctgatgttta ttcccgttga acccgctttt ttactggcgc
     8701 ttgaccgcca gccggagctg atcaccgaag cgttgaaaaa caatatcatg ctggttagcc
     8761 cgactacgct gctggtggcg ctgcgcacta tcgccaacct gtggcgttat gagcatcaaa
     8821 gccgcaacgc ccagcaaatc gccgatcgtg ccagcaagct gtacgacaag atgcgtttgt
     8881 tcgtcgatga catgtcagcg attggtcaaa gtctcgacaa agcgcaggat aactaccgcc
     8941 aggcaatgaa aaaactctct tcaggtcgcg gaaatgtgct ggcgcaggca gaagcgtttc
     9001 gcggtttagg ggtagaaatt aaacgcgaga ttaatccgga tttggctgaa caggcggtga
     9061 gccaggatga agagtatcga cttcggtcgg ttccggagca gccgaatgat gaagcttatc
     9121 aacgcgatga tgaatataat cagcagtcgc gctagcccat tgggggtagt taagccgggt
     9181 agaaatctag ggcatcgacg cccaatctgt tacacttctg gaacaatttt ttgatgagca
     9241 ggcattgaga tggtggataa gtcacaagaa acgacgcact ttggttttca gaccgtcgcg
     9301 aaggaacaaa aagcggatat ggtcgcccac gttttccatt ccgtggcatc aaaatacgat
     9361 gtcatgaatg atttgatgtc attcggtatt catcgtttgt ggaagcgatt cacgattgat
     9421 tgcagcggcg tacgccgtgg gcagaccgtg ctggatctgg ctggtggcac cggcgacctg
     9481 acagcgaaat tctctcgcct ggtcggagaa actggcaaag tggtccttgc tgatatcaat
     9541 gaatccatgc tcaaaatggg ccgcgagaag ctgcgtaata tcggtgtgat tggcaacgtt
     9601 gagtatgttc aggcgaacgc tgaggcgctg ccgttcccgg ataacacctt tgattgcatc
     9661 accatttcgt ttggtctgcg taacgtcacc gacaaagata aagcactgcg ttcaatgtat
     9721 cgcgtgctga aacccggcgg ccgcctgctg gtgcttgagt tctcgaagcc aattatcgag
     9781 ccgctgagca aagcctatga tgcatactcc ttccatgtgc tgccgcgtat tggctcactg
     9841 gtcgcgaacg acgccgacag ctaccgttat ctggcagaat ccatccgtat gcatcccgat
     9901 caggataccc tgaaaaccat gatgcaggat gccggattcg aaagtgtcga ctactacaat
     9961 ctgacggcag gggttgtggc gctgcatcgt ggttataagt tctgacagga gaccggaaat
    10021 gccttttaaa cctttagtga cggcaggaat tgaaagtctg ctcaacacct tcctgtatcg
    10081 ctcacccgcg ctgaaaacgg cccgctcgcg tctgttgggc aaagtgctgc gcgtggaggt
    10141 aaaaggcttt tcgacgtcat tgattctggt gttcagcgaa cgccaggttg atgtactggg
    10201 cgaatgggca ggcgatgctg actgcaccgt tatcgcctac gccagtgtgt tgccgaaact
    10261 tcgcgatcgc cagcagcttg ccgcactgat tcgcagtggt gagctggaag tgcagggcga
    10321 tattcaggtg gtgcaaaact tcgttgcgct ggcagatctg gctgagttcg accctgcgga
    10381 actgctggcc ccttataccg gtgatatcgc cgctgaaggt atcagcaaag ccctgcgcgg
    10441 gggcgcaaag ttcctgcatc acggcattaa gcgccagcaa cgttatgtgg cggaagccat
    10501 tactgaagag tggcgtatgg cacccggtcc gcttgaagtg gcctggtttg cggaagagac
    10561 ggctgccgtc gagcgtgctg ttgatgccct gaccaaacgg ctggaaaaac tggaggctaa
    10621 atgacgccag gtgaagtacg gcgcctatat ttcatcattc gcactttttt aagctacgga
    10681 cttgatgaac tgatccccaa aatgcgtatc accctgccgc tacggctatg gcgatactca
    10741 ttattctgga tgccaaatcg gcataaagac aaacctttag gtgagcgact acgactggcc
    10801 ctgcaagaac tgggaccggt atggatcaag ttcgggcaga tgttatcaac ccgccgcgat
    10861 ctttttccgc cgcatattgc cgatcagctg gcgttattgc aggacaaagt cgctccgttt
    10921 gatggcaagc tggcgaagca gcagattgaa gctgcaatgg gcggcttgcc ggtagaagcg
    10981 tggtttgacg attttgaaat caagccgctg gcttctgctt ctatcgccca ggttcatacc
    11041 gcgcgattga aatcgaatgg taaagaggtg gtgattaaag tcatccgccc ggatattttg
    11101 ccggtcatta aagcggatct gaaacttatc taccgtctgg ctcgctgggt gccgcgtttg
    11161 ctgccggatg gtcgccgtct gcgcccaacc gaagtggtgc gcgagtacga aaagaccttg
    11221 attgatgaac tgaatttgct gcgggaatct gccaacgcca ttcagcttcg acgcaatttt
    11281 gaagacagcc cgatgctcta catcccggaa gtttaccctg actattgtag tgaagggatg
    11341 atggtgatgg agcgtattta cggcattccg gtgtctgatg ttgcgacgct ggagaaaaac
    11401 ggcaccaaca tgaaattgct ggcggaacgc ggcgtgcagg tgttcttcac tcaggtcttt
    11461 cgcgacagct ttttccatgc cgatatgcac cctggcaaca tctttgtaag ctatgaacac
    11521 ccagaaaacc cgaaatatat cggcattgat tgcgggattg ttggctcgct aaacaaagaa
    11581 gataaacgct atctggcgga aaactttatc gctttcttta atcgcgatta tcgcaaagtt
    11641 gcagagctac acgtcgattc aggctgggtg ccgccagata ccaacgttga agagttcgaa
    11701 tttgccattc gtacggtctg tgaacctatc tttgagaaac cgttggccga aatttcgttt
    11761 ggacatgtgc tgttaaatct atttaatacg gcgcgtcgct tcaatatgga agtgcagccg
    11821 caactggtgt tactccagaa aaccctgctc tacgtcgaag gggtaggacg ccagctttat
    11881 ccgcagctcg atttatggaa aacggcgaag cctttccttg agtcgtggat taaagatcag
    11941 gtcggtattc ctgcgctggt gagagcattt aaagaaaaag cgccgttctg ggtcgaaaaa
    12001 atgccagaac tgcctgaact ggtttacgac agtttgcgcc agggcaagta tttacagcac
    12061 agtgttgata agattgcccg cgagcttcag tcaaatcatg tacgtcaggg acaatcgcgt
    12121 tattttctcg gaattggcgc tacgttagta ttaagtggca cattcttgtt ggtcagccga
    12181 cctgaatggg ggctgatgcc cggctggtta atggcaggtg gtctgatcgc ctggtttgtc
    12241 ggttggcgca aaacacgctg attttttcat cgctcaaggc gggccgtgta acgtataatg
    12301 cggccttgtt taatcatcat ctaccacaga ggaacatgta tgggtggtat cagtatttgg
    12361 cagttattga ttattgccgt catcgttgta ctgctttttg gcaccaaaaa gctcggctcc
    12421 atcggttccg atcttggtgc gtcgatcaaa ggctttaaaa aagcaatgag cgatgatgaa
    12481 ccaaagcagg ataaaaccag tcaggatgct gattttactg cgaaaactat cgccgataag
    12541 caggcggata cgaatcagga acaggctaaa acagaagacg cgaagcgcca cgataaagag
    12601 caggtgtaat ccgtgtttga tatcggtttt agcgaactgc tattggtatt catcatcggc
    12661 ctcgtcgttc tggggccgca acgactgcct gtggcggtaa aaacggtcgc gggctggatt
    12721 cgcgcgttgc gttcactggc gacaacggtg cagaacgaac tgacccagga gttaaaactc
    12781 caggagtttc aggacagtct gaaaaaggtt gaaaaggcga gcctcactaa cctgacgccc
    12841 gaactgaaag cgtcgatgga tgaactacgc caggccgcgg agtcgatgaa gcgttcctac
    12901 gttgcaaacg atcctgaaaa ggcgagcgat gaagcgcaca ccatccacaa cccggtggtg
    12961 aaagacaatg aagctgcgca tgaaggcgta acgcctgctg ctgcacaaac gcaagccagt
    13021 tcgccggaac agaagccaga aaccacgcca gagccggtgg taaaacctgc tgcggacgct
    13081 gaaccgaaaa ccgctgcacc ttccccttcg tcgagtgata aaccgtaaac atgtctgtag
    13141 aagatactca accgcttatc acgcatctga ttgagctgcg taagcgtctg ctgaactgca
    13201 ttatcgcggt gatcgtgata ttcctgtgtc tggtctattt cgccaatgac atctatcacc
    13261 tggtatccgc gccattgatc aagcagttgc cgcaaggttc aacgatgatc gccaccgacg
    13321 tggcctcgcc gttctttacg ccgatcaagc tgacctttat ggtgtcgctg attctgtcag
    13381 cgccggtgat tctctatcag gtgtgggcgt ttatcgcccc agcgctgtat aagcatgaac
    13441 gtcgcctggt ggtgccgctg ctggtttcca gctctctgtt gttttatatc ggcatggcgt
    13501 tcgcctactt tgtggtcttt ccgctggcat ttggcttcct tgccaatacc gcgccggaag
    13561 gggtacaggt atccaccgac atcgccagct atttaagctt cgttatggcg ctgtttatgg
    13621 cgtttggtgt ctcctttgaa gtgccggtgg caatagtgct gctgtgctgg atgggaatta
    13681 cctcgccaga agacttacgc aaaaaacgtc cgtatgtgct ggttggcgca tttgttgtcg
    13741 ggatgctgct gacgccgccg gatgtcttct cgcaaacgct gctggcgatc ccgatgtatt
    13801 gcctgtttga aatcggtgtc ttcttctcac gcttttacgt tggtaaaggg cgaaatcggg
    13861 aagaggaaaa cgacgctgaa gcagaaagcg aaaaaactga agaataaatt caaccgcccg
    13921 tcagggcggt tgtcatatgg agtacaggat gtttgatatc ggcgttaatt tgaccagttc
    13981 gcaatttgcg aaagaccgtg atgatgttgt agcgcgcgct tttgacgcgg gagttaatgg
    14041 gctactcatc accggcacta acctgcgtga aagccagcag gcgcaaaagc tggcgcgtca
    14101 gtattcgtcc tgttggtcaa cggcgggcgt acatcctcac gacagcagcc agtggcaagc
    14161 tgcgactgaa gaagcgatta ttgagctggc cgcgcagcca gaagtggtgg cgattggtga
    14221 atgtggtctc gactttaacc gcaacttttc gacgccggaa gaacaggaac gcgcttttgt
    14281 tgcccagcta cgcattgccg cagaattaaa catgccggta tttatgcact gtcgcgatgc
    14341 ccacgagcgg tttatgacat tgctggagcc gtggctggat aaagtgcctg gtgcggttct
    14401 tcattgcttt accggcacac gcgaagagat gcaggcgtgc gtggcgcatg gaatttatat
    14461 cggcattacc ggttgggttt gcgatgaacg acgcgggctg gagctgcggg aattgttgcc
    14521 gttgattccg gcggagaaat tgctgatcga aactgatgcg ccgtatctgc tccctcgcga
    14581 tctcacgcca aagccatcat cccggcgcaa cgagccagcc catctgcccc atattttgca
    14641 acgtattgcg cactggcgtg gagaagatgc cgcatggctg gctgccacca cggatgctaa
    14701 tgtcaaaaca ctgtttggga ttgcgtttta gagtttgcgg aactcggtgt tcttcacact
    14761 gtgcttaatc tctttattaa taagattcag caatagcatg gagcgagcct cgccgtctgg
    14821 ctcggtgaaa atggcctgaa aaccttcgaa tgcgccttca gtaataatca ctttatcacc
    14881 aggatacggc gtcgatggat cgacaatatc tttcggttta tacactgata actgatgaat
    14941 caccgccgat gggactatcg ctggcgacgc gccaaagcgc acgaagtggc tgacaccgcg
    15001 ggtcgcgttg atagtcgtgg tatgaatcac ttccggatcg aactccacaa acaagtagtt
    15061 ggggaacaat ggctcactga ctgcagtacg tttgccacgc acgatttttt ccagggtgat
    15121 catcggtgcc aggcaattca cagcctgtct ttcgaggtgt tcctgggcac gttgaagttg
    15181 cccgcgcttg cagtacagta aataccagga ttgcataatg actcttatcc gtttaatcgg
    15241 ggcgcaagga tagcaaaagc tttacgctaa gttaattata ttccccggtt tgcgttatac
    15301 cgtcagagtt cacgctaatt taacaaattt acagcatcgc aaagatgaac gccgtataat
    15361 gggcgcagat taagaggcta caatggacgc catgaaatat aacgatttac gcgacttctt
    15421 gacgctgctt gaacagcagg gtgagctaaa acgtatcacg ctcccggtgg atccgcacct
    15481 ggaaatcact gaaattgctg accgcacttt gcgtgccggt gggcctgcgc tgttgttcga
    15541 aaaccctaaa ggctactcaa tgccggtgct gtgcaacctg ttcggtacgc caaagcgcgt
    15601 ggcgatgggc atggggcagg aagatgtttc ggcgctgcgt gaagttggta aattattggc
    15661 gtttctgaaa gagccggagc cgccaaaagg tttccgcgac ctgtttgata aactgccgca
    15721 gtttaagcaa gtattgaaca tgccgacaaa gcggctgcgt ggtgcgcctt gccaacaaaa
    15781 aatcgtctct ggcgatgacg tcgatctcaa tcgtattccc attatgacct gctggccgga
    15841 agatgccgcg ccgctgatta cctgggggct caccgtaacg cgcggcccgc ataaagagcg
    15901 gcagaatctg ggcatttatc gccagcagct aattggtaaa aacaaactga ttatgcgctg
    15961 gctgtcgcat cgcggcggcg cgttggatta tcaggagtgg tgtgcggcgc atccgggcga
    16021 acgtttcccg gtttctgtgg cgctgggtgc cgatcccgcc acgattctcg gtgcagtcac
    16081 ccccgttccg gatacgcttt cagagtatgc gtttgccgga ttgctgcgcg gcaccaagac
    16141 cgaagtggtg aagtgtatct ccaatgacct tgaagtgccc gccagtgcgg agattgtgct
    16201 ggaagggtat atcgaacaag gcgaaactgc gccggaaggg ccgtatggcg accacaccgg
    16261 ttactataac gaagtcgata gttttccggt atttaccgtg acgcatatta cccagcgtga
    16321 agatgcgatt tatcattcca cctataccgg gcgtccgcca gatgaacctg cggtactggg
    16381 tgtagcactg aacgaagtgt tcgtgccgat tctgcaaaaa cagttcccgg aaattgtcga
    16441 tttttatctg ccgccggaag gctgttctta tcgtctggcg gtagtgacga tcaaaaaaca
    16501 gtatgccgga cacgcgaagc gcgtcatgat gggcgtctgg tcgttcttac gccagtttat
    16561 gtacactaaa tttgtgatcg tttgcgatga tgacgtcaac gcccgcgact ggaacgatgt
    16621 gatttgggcg attaccaccc gtatggaccc ggcgcgggat accgttctgg tagaaaatac
    16681 gcctattgat tatctggatt ttgcctcccc tgtctccggg ctgggttcaa aaatggggct
    16741 ggatgccacg aataaatggc cgggggaaac ccagcgtgaa tggggacgtc ccatcaaaaa
    16801 agatccagat gttgtcgcgc atattgacgc catttgggat gaactggcta tttttaacaa
    16861 cggtaaaagc gcctgatgcg cgtttgtttt gccctattta tcgatccgac agagaaagcg
    16921 catgacaacc ttaagctgta aagtgacctc ggtagaagct atcacggata ccgtatatcg
    16981 tgtccgcatc gtgccagacg cggccttttc ttttcgtgct ggtcagtatt tgatggtagt
    17041 gatggatgag cgcgacaaac gtccgttctc aatggcttcg acgccagatg aaaaagggtt
    17101 tatcgagctg catattggcg cttctgaaat caacctttac gcgaaagcag tcatggaccg
    17161 catcctcaaa gatcatcaaa tcgtggtcga tattccccac ggagaagcgt ggctgcgcga
    17221 tgatgaagag cgtccgatga ttttgattgc gggcggcacc gggttctctt atgcccgctc
    17281 gattttgctg acagcgttgg cgcgtaaccc aaaccgtgat atcaccattt actggggcgg
    17341 gcgtgaagag cagcatctgt atgatctctg cgagcttgag gcgctttcgt tgaagcatcc
    17401 tggtctacaa gtggtgccgg tggttgaaca accggaagcg ggctggcgtg ggcgtactgg
    17461 caccgtgtta acggcggtat tgcaggatca tggtacgctg gcagagcatg atatctatat
    17521 tgccggacgt tttgagatgg cgaaaattgc ccgtgacctg ttttgcagcg agcgtaatgc
    17581 gcgggaagat cgcctgtttg gcgatgcgtt tgcatttatc tgagatataa aaaacccgcc
    17641 cctgacaggc gggaagaacg gcaactaaaa ctttatacag tggcatttag atctatgtcg
    17701 catccggcat tcggcacacg ctgcctgatg cgacgctggc gcgtcttatc aggcctacaa
    17761 ttggtgcagg atcggtagac cggataaggc gttcacgccg catctggcaa gtggttaaat
    17821 ccgctcaaac accgtcgcaa taccctgacc cagaccgata cacatcgtcg caagaccaaa
    17881 ctgggcatcc ttgcgctcca tgagatttag cagcgtgctg ctgatacgcg caccggaaca
    17941 gcccaacgga tgacccagcg cgatcgcgcc accgttgagg ttgatcttct cgtcaatctg
    18001 ctcaatcagt cccagatctt taatacatgg caggatctgc gcggcaaagg cttcattcat
    18061 ttcaaacacg ccgatatcac tgacagaaag ccccgctttt ttcagcgcca attttgaggc
    18121 cggaaccgga ccgtaaccca taatcgacgg atcacaacca acgaccgcca tcgaacgcac
    18181 acgggcgcgc ggcttaagac ctaattcatg ggcgcggctt tcactcatca ccagcatggc
    18241 agctgcgcca tcggaaagcg cagaagatgt acccgccgtt accgtaccgc tgacgggatc
    18301 aaacgccgga cgcagcgtgg cgagggcttc cacggtggtt tccgggcgaa tcacttcgtc
    18361 gtaattaaac tgcgtcagga cgccgtcggc atcgtgacca ccggtcggga taatttcatt
    18421 tttaaatgca cccgactgcg tggcggccca ggcgcgggcg tgtgaccgcg cggcaaaggc
    18481 atcctgcatt tcacggctga taccgtgcat acgcgccagc atttctgccg ttaagcccat
    18541 catgcccgcc gctttggcga cattacggct aaggccgggg tgaaaatcga cgccgtgact
    18601 cattggcaca tggcccatat gctccacgcc gccaaccaga catgtctgcg catcgccgat
    18661 catgatcatt cgtgctgcgt catgcagtgc ctgcatggat gaaccacaca agcgattgac
    18721 agtaaccgcc gggacagagt gcggcacttc tgccaacagc gccgcgttac gggcgatatt
    18781 aaaaccctgc tccagcgtct gctgtacaca accccagtaa atatcgtcga gggccgccgc
    18841 ttccagcgcc gggttacgcg ccagcaggct acgcattaaa tgagcggaga gatcttccgc
    18901 acgcacgtga cgaaaagcac cgcccttcga acggcccatc ggggtgcgaa ttgcatcgac
    18961 aatgacaacc tgttccattg tgactcctta agccattttc aggtcgccaa ccggacgggc
    19021 tggctcaacc gggggatagt acggttcgtt atgacgcgct ttattacgca gaccttccgg
    19081 cacttcatac agcgggccga gatgctgata ttgctgtgcc atatcgaggt attttgcgct
    19141 accgagggtg tccagccagc ggaacgcgcc gccgtgaaac ggagggaagc ccaggccgta
    19201 gaccagcgcc atatccgctt ctgccggagt ggcgataatg ccttcttcca gacagcgcac
    19261 cacttcgttg accatcggga tcatcatgcg ggcgataatc tcttcttcgc tgaaatcgcg
    19321 cttcggctgg ctcacttctg ccagcaggtc gtcaacgacg gcatcttctt ctttcttcgg
    19381 cttacctttg ctgtcttctt tataacgcca gaaaccgagg ccgttcttct gaccaaagcg
    19441 gttggcatta aacagcgcgt cgatggcatc gcgataatct ttctgcatcc gctgcgggaa
    19501 gcctgctgcc atgacagcct gggcgtgatg cgcggtgtca atgcccacaa catccagcag
    19561 atatgccgga cccatcggcc agccaaactg tttttccatc actttgtcga tcttgcggaa
    19621 atccgcgccg tcgcgcagca actggctgaa accggcgaaa taggggaaca gtacacggtt
    19681 gacgaagaag ccggggcagt cgttaaccac gatcggcgtc ttgcccatct tgctcgccca
    19741 tgcgacgact ttcgcgatgg tttcgtcgga gcttttctcg ccgcgaataa tttccaccaa
    19801 cggcatccgg tgcaccgggt taaagaagtg catcccgcag aagttttccg gacgttccag
    19861 cgcgttggcc agttcgctga taggaatggt tgaagtgtta gacgccagca cggtatccgg
    19921 gcgtactttc tgctcggttt ctgccagtac ggcttttttc actttcgggt tttcgacaac
    19981 cgcttctacc acaacatcca cgcggtcaaa accagagtaa tcgagcgttg ggtggattgt
    20041 ggagatcacg ccagccagtt tcagaccatc gatcttgccg cgctcaagct gcttgttcag
    20101 cagtttcgcg gcttcggtca tgccgagggt taacgacttg tcgttgatat ctttcatgac
    20161 aaccggtacg cctttccacg cagactggta agcgatgccg ccacccataa tgcctgcgcc
    20221 cagcacagcg gcctgtttcg gggtttcaac gtctttggtg agtttcttcg ctttgccttt
    20281 tacatattga tcgttaagga aaatgccgac cagtgcgcga gcttcgttgg tatgcgccag
    20341 cgggacaaaa cttttgtttt ccagatttaa tgcttcttca cgaccaaaac gggccgccgc
    20401 ttcaatggtt tttactgcgg tgatgggggc cggataatgt ttccccgccg tttgtgcgac
    20461 catcccttta gcgatggtga aactcatggt ggcttcaatc ttgctcagtt ttagcggttc
    20521 cagcttcggc tgacgtttcg ctttccagtc gaggtcgccg ttaatggcct ggcgtaaaat
    20581 cgccatcgcc ccttcaacca gtttttctgc tttgactaca ccatccacca gaccgatttt
    20641 cagcgcctga tccgcgccga catctttacc ggcggcaatg atttccagcg cactgtcagc
    20701 gcccagcata cgtggcatac gtacagaacc gccaaagccc ggcatgatgc ccagtttggt
    20761 ttccggcaga ccgatgcgca gatccggcgt cgccagacga taatcggtcg ccagcacgca
    20821 ttcgcagcca ccgcccagcg catagccgtt gacggcagca atggtcggca ccgggagatc
    20881 ttccaggcga ttaaacacgc tattggcaaa gtgcagccac tgacttaact gttcttcagg
    20941 aacgaggaac agggacaaaa attcggtgat atcagcaccg acgataaagg ctgctttgtt
    21001 cgaacgcagc agcagccctt ttagatctga ttgctgttcc agcacgccga tggcctcgcc
    21061 gaggctggcg acggtggcag tgtcgagttt attaactgaa cctggggcat caaataccag
    21121 ttcggcaatg ccatcttcca gccagtcaag gtacagggtg tcgcctttgt aaagcatgtc
    21181 agtctcctga atccgcaagg tgatctggtc gtaccagatg catcgaagtg tgtattttgt
    21241 gttaaaaata tgcaaataaa agattaaaga aatgccgatc tgatcacgct cggcagaaat
    21301 cacgctctgg atgaacgatg tgctaagatg cggagactta aggtcaaaaa aacagaaggg
    21361 taaaaaatgg aatcactggc ctcgctctat aaaaatcata tagctacctt acaggaacgg
    21421 actcgcgatg cgctggcgcg cttcaagctg gatgcgttac ttattcactc cggcgaactg
    21481 ttcaacgttt ttctcgacga tcatccctat ccgtttaaag tgaacccgca attcaaagcg
    21541 tgggtgccag taactcaggt gccaaactgc tggctgctgg tggatggcgt gaataagccg
    21601 aaactgtggt tctatctacc ggttgattac tggcacaacg tcgaaccgct gccgaactcc
    21661 ttctggactg aagatgtaga agtgatcgcg ctgccgaaag ccgatggcat tggtagtctg
    21721 ttgcctgctg cgcgcggcaa tatcggttat atcggtccgg tgccagaacg tgcgctgcaa
    21781 ctgggtattg aggccagcaa tatcaacccg aaaggggtga tcgactacct gcattactat
    21841 cgctccttca aaaccgagta cgaactggcc tgtatgcgtg aagcgcagaa aatggcggtc
    21901 aacggtcatc gcgcggcaga agaagcgttc cgttctggca tgagcgagtt cgatatcaac
    21961 attgcctatc tgaccgcgac cggtcatcgt gataccgacg taccttatag caacattgtg
    22021 gcgcttaacg aacacgctgc ggtgttgcat tacaccaaac tggaccacca ggcaccggaa
    22081 gagatgcgca gcttcctgct ggatgccggg gccgaatata acggctatgc ggctgatcta
    22141 acccgtacct ggtcggcaaa aagcgacaac gactacgcac agctggtgaa agacgtaaat
    22201 gatgaacaac tggcgctgat cgctaccatg aaagcaggcg tcagctatgt ggattaccat
    22261 atccagttcc atcagcgcat cgccaaactg ctgcgtaaac atcaaatcat caccgatatg
    22321 agtgaagaag cgatggtcga aaacgatctc accggaccgt ttatgccgca cggtatcggc
    22381 catccgctgg gcctgcaggt gcatgacgta gccggtttta tgcaggatga tagcggtacg
    22441 cacctcgcgg caccggcaaa atatccgtac ctgcgctgca cccgtattct ccagccgggc
    22501 atggtgttaa ccatcgaacc gggtatctac ttcatcgaat cgcttctggc accgtggcgt
    22561 gaagggcagt tcagcaaaca cttcaactgg cagaaaattg aagcactgaa accgttcggc
    22621 ggcattcgta tcgaagacaa cgtggtgatt cacgaaaaca acgtggaaaa cattacccgg
    22681 gatctgaaac tggcgtgatg gaaagctggt taattcctgc ggcaccggtc acggtcgttg
    22741 aagagatcaa aaagagccgc ttcattacgc tgttggcgca taccgatggc gttgaggcgg
    22801 caaaagcgtt tgttgaatcg gtgcgggcag agcaccccga tgcccgccac cattgcgtgg
    22861 cgtgggtggc gggtgcgccg gatgattctc aacagctggg tttctctgac gacggggagc
    22921 cggcgggaac ggcaggtaaa ccgatgctcg cccaactaat gggcagcggc gtcggggaaa
    22981 ttaccgctgt ggtggtgcgc tactacggcg gcatattgct tggcaccggt gggttagtga
    23041 aagcgtatgg cggcggcgtg aatcaggcgc tgcgccagct aacgacccaa cgcaagacac
    23101 cattaaccga atatactttg caatgtgaat atagtcagtt aaccggcatt gaagcgttgc
    23161 tggggcagtg tgacggcaaa attattaaca gtgattatca ggcattcgtt ctgctgcggg
    23221 tggcgcttcc ggcggcgaaa gtggctgaat tttccgcaaa gctggcggat tttagccgtg
    23281 gttcattgca attgttagcg attgaagaat aatccccact tcgttttgca gaactaagga
    23341 agcggcagag atgcattttc gcgccattac ccgaatcgtt ggactactgg tcatcttatt
    23401 ttcagggacc atgattatcc ctgggctggt agcactcatc taccgggatg gagcgggccg
    23461 cgcttttacc cagacctttt ttgtcgccct cgccattggc tctatgctgt ggtggccgaa
    23521 ccgcaaagag aagggcgaac tgaaatcccg tgaggggttt ctgatagtgg tgctgttctg
    23581 gaccgtgctg ggtagcgtcg gtgcgctccc ttttatcttc tcggaaagcc cgaacctcac
    23641 gattaccgat gcgttttttg aatctttctc tggcctgacc accacggggg ccactacgct
    23701 agtggggctg gattcgctcc ctcacgccat cctcttttat cgccagatgc tgcaatggtt
    23761 tggcgggatg gggatcatcg tgttagcggt tgcgatactg cctatcctcg gcgtgggtgg
    23821 gatgcagctc tatcgcgcag aaatgcccgg cccgctgaaa gataacaaaa tgcgcccgcg
    23881 aattgcggaa acggcgaaaa ccctgtggtt gatttatgtc ttgctgaccg tcgcctgtgc
    23941 gctggcgttg tggtttgccg gaatggatgc ctttgatgcc atcggccata gctttgcgac
    24001 tatcgctatt ggcggcttct cgacacatga tgccagtatc ggttatttcg acagcccgac
    24061 tattaacact atcattgcta tcttcctgct gatctccggc tgtaactacg gtctgcactt
    24121 ttcactgtta agtgggcgta gtctgaaggt ttattggcgc gatccggaat ttcgcatgtt
    24181 tatcggcgta cagtttacgc tggtggttat ttgtacactc gtactgtggt ttcataatgt
    24241 ctacagttcg gcgctgatga caattaacca ggcgtttttc caggtggtat cgatggcgac
    24301 aaccgccggg tttacgactg acagcattgc ccgctggccg ctctttttgc cggtactgct
    24361 cttatgttca gcttttatcg gcggttgcgc cgggtcaacg ggcggtggcc tgaaagtgat
    24421 ccgcatcctg ctgctgttta agcaggggaa ccgtgagctg aaacgactgg tgcatccgaa
    24481 cgccgtctat agcattaagc tggggaatcg cgcactgccg gaacgtatcc tcgaagccgt
    24541 gtggggattt ttctccgcct atgcattggt gtttattgtc agtatgctgg cgattatcgc
    24601 cacgggcgtg gatgactttt ctgcctttgc ctcggttgtt gcgacattga ataacctggg
    24661 gccagggctt ggtgtggttg ctgataactt taccagtatg aacccggtgg ctaaatggat
    24721 cctgattgcc aacatgctgt ttggtcgtct cgaggtcttt acattgctgg tgctctttac
    24781 cccgactttc tggcgtgaat gatggagtaa tacgtgaaaa cattaattct tttctcaaca
    24841 agggacggac aaacgcgcga gattgcctcc tacctggctt cggaactgaa agaactgggg
    24901 atccaggcgg atgtcgccaa tgtgcaccgc attgaagaac cacagtggga aaactacgac
    24961 cgtgtggtca ttggtgcttc tattcgctat ggtcactacc actctgcgtt ccaggagttt
    25021 gtcaaaaagc atgcgacgcg actgaattcg atgccgagcg ccttttactc cgtaaacctg
    25081 gtggcgcgta aaccggagaa gcgtactcca cagaccaaca gctacgcgcg gaagtttctg
    25141 atgagttcac aatggcgtcc cgatcgctgt gccgtcattg ccggggcgct gcgttaccca
    25201 cgttatcgct ggtacgaccg ttttatgatc aagctgatta tgaagatgtc aggcggtgaa
    25261 acggatacgc gcaaagaagt tgtctatacc gattgggagc aggtggcgaa tttcgcccga
    25321 gaaatcgccc atttaaccga caaaccgacg ctgaaataag cataaagaat aaaaaatgtg
    25381 cggtcagaaa attattttaa atttcctctt gtcaggccgg aataactccc tataatgcgc
    25441 caccactgac acggaacaac ggcaaacacg ccgccgggtc agcggggttc tcctgagaat
    25501 ctcaacagag aaaagcaaaa aaatgcttga ctctgtagcg ggaaggcgta ttatgcacac
    25561 cccgcgccgc tgagaaaaag tgaagcggca ctgctcttta acaatttatc agacaatctg
    25621 tgtgggcact cga
//
LOCUS       DAZFLD010000057        24835 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.057, whole genome shotgun sequence.
ACCESSION   DAZFLD010000057 DAZFLD010000000
VERSION     DAZFLD010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24835
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.057"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1620
                     /gene="yqiK"
                     /locus_tag="ACHKBL_001938"
     CDS             <1..1620
                     /gene="yqiK"
                     /locus_tag="ACHKBL_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HFO5818817.1"
                     /translation="AIIAVCILFIIGIIFARLYRRASAEQAFVRTGLGGQKVVMSGGA
                     IVMPIFHEIIPINMNTLKLEVSRSTIDSLITKDRMRVDVVVAFFVRVKPSVEGIATAA
                     QTLGQRTLSPEDLRMLVEDKFVDALRATAAQMTMHELQDTRENFVQGVQNTVAEDLSK
                     NGLELESVSLTNFNQTSKEHFNPNNAFDAEGLTKLTQETERRRRERNEVEQDVEVAVR
                     EKNRDALSRKLEIEQQEAFMTLEQEQQVKTRTAEQNAKIAAFEAERRREAEQTRILAE
                     RQIQETEIDREQAVRSRKVEAEREVRIKEIEQQQVTEIANQTKSIAIAAKSEQQSQAE
                     ARANLALAEAVSAQQNVETTRQTAEADRAKQVALIAAAQDAETKAVELTVRAKAEKEA
                     AEMQAAAIVELAEATRKKGLAEAEAQRALNDAINVLSDEQTSLKFKLALLQALPAVIE
                     KSVEPMKSIDGIKIIQVDGLNRGGATGDANTGNVGGGNLAEQALSAALSYRTQAPLID
                     SLLNEIGVSGGSLAALTSSLSSTTPVEEKAE"
     gene            1860..1919
                     /locus_tag="ACHKBL_001939"
     CDS             1860..1919
                     /locus_tag="ACHKBL_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFO5818818.1"
                     /translation="MMKLVIILIVLLLVSFAAY"
     gene            complement(2039..3472)
                     /gene="hldE"
                     /locus_tag="ACHKBL_001940"
     CDS             complement(2039..3472)
                     /gene="hldE"
                     /locus_tag="ACHKBL_001940"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HFO5818819.1"
                     /translation="MKVTLPEFERAGVMVVGDVMLDRYWYGPTSRISPEAPVPVVKVN
                     TIEERPGGAANVAMNIASLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHP
                     TITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASV
                     QQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMK
                     LIADYELSALLVTRSEQGMSLLQPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAA
                     GNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGFGVMTEEELKLAVAA
                     ARKRGEKVVMTNGVFDILHAGHVSYLANARKLGDRLIVAVNSDASTKRLKGDSRPVNQ
                     LEQRMIVLGALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANG
                     GEVLVLNFEDGCSTTNIIKKIQQDKKG"
     gene            complement(3520..6360)
                     /gene="glnE"
                     /locus_tag="ACHKBL_001941"
     CDS             complement(3520..6360)
                     /gene="glnE"
                     /locus_tag="ACHKBL_001941"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HFO5818820.1"
                     /translation="MKPLSSPLQQYWQTVVERLPEPLAEESLSAQAKSVLTFSDFLQD
                     SIIAHPEWLTELESQPPQADEWQHYAAWLQEALSNVSDEAGLMRELRLFRRRIMVRIA
                     WAQTLALVTEESILQQLSHLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGM
                     GKLGGGELNFSSDIDLIFAWPEHGCTQGGRRELDNAQFFTRMGQRLIKVLDQPTQDGF
                     VYRVDMRLRPFGESGPLVLSFAALEDYYQEQGRDWERYAMVKARIMGDSDGVYANELR
                     AMLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQ
                     LIRGGREPSLQSRSLLPTLSAIAALHLLSENDAEQLRVAYLFLRRLENLLQSINDEQT
                     QTLPFDELNRARLAWAMDFADWPQLTGVLTAHMANVRRVFNELIGDDESETQEESLSE
                     QWRELWQDALQEDDTTPVLAHLSEDDRKQVLMLIADFRKELDKRTIGPRGRQVLDHLM
                     PHLLSDVCAREDAAVTLSRITALLVGIVTRTTYLELLSEFPAALKHLISLCAASPMIA
                     SQLARYPLLLDELLDPNTLYQPTATDAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQ
                     LLRIAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQQAWVQMVARYGKPNHLNEREGRG
                     FAVVGYGKLGGWELGYSSDLDLIFLHDCPMDAMTDGEREIDGRQFYLRLAQRIMHLFS
                     TRTSSGILYEVDARLRPSGAAGMLVTSAEAFADYQKNEAWTWEHQALVRARVVYGDPQ
                     LTAHFDAVRREIMTLPREGKTLQTEVREMREKMRAHLGNKHRDRFDIKADEGGITDIE
                     FITQYLVLRYAHEKPKLTRWSDNVRILELLAQNDIMEEQEAMALTRAYTTLRDELHHL
                     ALQELPGHVSEDCFTAERELVRASWQKWLVEE"
     gene            complement(6383..7684)
                     /gene="ygiF"
                     /locus_tag="ACHKBL_001942"
     CDS             complement(6383..7684)
                     /gene="ygiF"
                     /locus_tag="ACHKBL_001942"
                     /EC_number="3.6.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic triphosphatase"
                     /protein_id="HFO5818821.1"
                     /translation="MAQEIELKFIVNHSAVEALRDHLNTLGGEHHDPVQLLNIYYETP
                     DNWLRGHDMGLRIRGENGRYEMTMKVAGRVTGGLHQRPEYNVALSEPTLDLAQLPTEV
                     WPNGELPTDLASRVQPLFSTDFYREKWLVEVDGSQIEIALDQGEVKAGEFAEPICELE
                     LELLSGDTRAVLKLANQLVSQTGLRQGSLSKAARGYHLAQGNPAREIKPTTILHVAAK
                     ADVEQGLEAALELALAQWQYHEELWVRGNDAAKEQVLAAIGLVRHTLMLFGGIVPRKA
                     STHLRDLLTQCEATIASAVSAVTAVYSTETAMAKLALTEWLVSKAWQPFLDAKAQSKM
                     SDSFKRFADIHLSRHAAELKSVFCQPLGDRYRDQLPRLTRDIDSILLLAGYYDPVVAQ
                     AWLENWQGLRHAIATGQRIEIEHFRNEANNQEPFWLHSGKR"
     gene            7926..8546
                     /gene="ygiM"
                     /locus_tag="ACHKBL_001943"
     CDS             7926..8546
                     /gene="ygiM"
                     /locus_tag="ACHKBL_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04211 family SH3 domain-containing protein"
                     /protein_id="HFO5818822.1"
                     /translation="MPKLRLIGLTLLALSATAVSHAEETRYVSDELNTWVRSGPGDHY
                     RLVGTVNAGEEVTLLQTDANTNYAQVKDSSGRTAWIPLKQLSTEPSLRSRVPDLENQV
                     KTLTDKLTNIDNTWNQRTAEMQQKVAQSDSVINGLKEENQKLKNELIVAQKKVDAASV
                     QLDDKQRTIIMQWFMYGGGVLGLGLLLGLVLPHLIPSRKRKDRWMN"
     gene            8610..9848
                     /gene="cca"
                     /locus_tag="ACHKBL_001944"
     CDS             8610..9848
                     /gene="cca"
                     /locus_tag="ACHKBL_001944"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused tRNA nucleotidyltransferase/2',3'-cyclic
                     phosphodiesterase/2' nucleotidase/phosphatase Cca"
                     /protein_id="HFO5818823.1"
                     /translation="MKIYLVGGAVRDALLGLPVKDRDWVVVGSTPQEMLDAGYQQVGR
                     DFPVFLHPQTHEEYALARTERKSGSGYTGFTCYAAPDVTLEDDLKRRDLTINALAQDD
                     NGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLSFRIADETLALM
                     REMTHAGELEHLTPERVWKETENALTTRNPQVFFQVLRDCGALRVLFPEIDALFGVPA
                     PARWHPEIDTGIHTLMTLSMAAMLSPQVDVRFATLCHDLGKGLTPPELWPRHHGHGPA
                     GVKLVEQLCQRLRVPNEIRDLARLVAEFHDLIHTFPMLNPKTIVKLFDSIDAWRKPQR
                     VEQLALTSEADVRGRTGFESADYPQGRWLREAWEVAQSVPTKAVVEAGFKGVEIREEL
                     TRRRIAAVAGWKEQRCPKPE"
     gene            complement(10036..10857)
                     /gene="bacA"
                     /locus_tag="ACHKBL_001945"
     CDS             complement(10036..10857)
                     /gene="bacA"
                     /locus_tag="ACHKBL_001945"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFO5818824.1"
                     /translation="MSDMHSLLIAAILGVVEGLTEFLPVSSTGHMIIVGHLLGFEGDT
                     AKTFEVVIQLGSILAVVVMFWRRLFGLIGIHFGRPLQHEGESKGRLTLIHILLGMIPA
                     VVLGLLFHDTIKSLFNPINVMYALVVGGLLLIAAECLKPKEPRAPGLDDMTYRQAFMI
                     GCFQCLALWPGFSRSGATISGGMLMGVSRYAASEFSFLLAVPMMMGATALDLYKSWGF
                     LTTGDIPMFAVGFITAFVVALIAIKTFLQLIKRISFIPFAIYRFIVAAAVYVVFF"
     gene            complement(10948..11316)
                     /gene="folB"
                     /locus_tag="ACHKBL_001946"
     CDS             complement(10948..11316)
                     /gene="folB"
                     /locus_tag="ACHKBL_001946"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dihydroneopterin
                     aldolase/7,8-dihydroneopterin epimerase"
                     /protein_id="HFO5818825.1"
                     /translation="MDIVFIEQLSVITTIGVYDWEQTIEQKLVFDIEMAWDNRKAAKS
                     DDVADCLSYADIAETVVSHVEGARFALVERVAEEVAELLLARFNSPWVRIKLSKPGAV
                     ARAANVGVIIERGNNLKENN"
     gene            11421..12038
                     /gene="plsY"
                     /locus_tag="ACHKBL_001947"
     CDS             11421..12038
                     /gene="plsY"
                     /locus_tag="ACHKBL_001947"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HFO5818826.1"
                     /translation="MSAIAPGMILIAYLCGSISSAILVCRLCGLPDPRTSGSGNPGAT
                     NVLRIGGKGAAVAVLIFDVLKGMLPVWGAYELGVSPFWLGLIAIAACLGHIWPVFFGF
                     KGGKGVATAFGAIAPIGWDLTGVMAGTWLLTVLLSGYSSLGAIVSALIAPFYVWWFKP
                     QFTFPVSMLSCLILLRHHDNIQRLWRRQETKIWTKFKRKREKDPE"
     gene            complement(12051..12983)
                     /gene="ttdR"
                     /locus_tag="ACHKBL_001948"
                     /gene_synonym="dan"
     CDS             complement(12051..12983)
                     /gene="ttdR"
                     /locus_tag="ACHKBL_001948"
                     /gene_synonym="dan"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate utilization transcriptional activator
                     TtdR"
                     /protein_id="HFO5818827.1"
                     /translation="MLNSWPLAKDLQVLVEIVHSGSFSAAAATLGQTPAFVTKRIQIL
                     ENTLATTLLNRSARGVALTESGQRCYEHALEILTQYQRLVDDVTQIKTRPEGMIRIGC
                     SFGFGRSHIAPAITELMRNYPELQVHFELFDRQIDLVQDNIDLDIRINDAIPDYYIAH
                     LLTKNKRILCAAPEYLQKYPQPQSLQELSRHDCLVTKERDMTHGIWELGNGQEKKSVK
                     VSGHLSSNSGEIVLQWALEGKGIMLRSEWDVLPFLESGKLVRVLPEYAQSANIWAVYR
                     EPLYRSMKLRVCVEFLAAWCQQRLGKPDEGYQVM"
     gene            13190..14101
                     /gene="ttdA"
                     /locus_tag="ACHKBL_001949"
     CDS             13190..14101
                     /gene="ttdA"
                     /locus_tag="ACHKBL_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008730 - L(+)-tartrate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
                     /protein_id="HFO5818828.1"
                     /translation="MMSESNKQQAVNKLTEIVANFTAMISTRMPDDVVDKLKQLKDAE
                     TSSMGKIIYHTMFDNMQKAIDLNRPACQDTGEIMFFVKVGSRFPLLGELQSILKQAVE
                     EATVKAPLRHNAVEIFDEVNTGKNTGSGVPWVTWDIVPDGDDAEIEVYMAGGGCTLPG
                     RSKVLMPSEGYEGVVKFVFENISTLAVNACPPVLVGVGIATSVETAAVLSRKAILRPI
                     GSRHPNPKAAELELRLEEGLNRLGIGPQGLTGNSSVMGVHIESAARHPSTIGVAVSTG
                     CWAHRRGTLLVHADLTFENLSHTRSAL"
     gene            14098..14703
                     /gene="ttdB"
                     /locus_tag="ACHKBL_001950"
     CDS             14098..14703
                     /gene="ttdB"
                     /locus_tag="ACHKBL_001950"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HFO5818829.1"
                     /translation="MKKILTTPIKAEDLQDIRVGDVIYLTGTLVTCRDVCHRRLIELK
                     RPIPYDLNGKAIFHAGPIVRKNGDKWEMVSVGPTTSMRMESFEREFIEQTGVKLVVGK
                     GGMGPLTEEGCQKFKALHVIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRVKEF
                     GPLIVSIDTHGNNLIAENKKLFAERRDPIVEEICEHVHYIK"
     gene            14752..16215
                     /gene="ttdT"
                     /locus_tag="ACHKBL_001951"
     CDS             14752..16215
                     /gene="ttdT"
                     /locus_tag="ACHKBL_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate/succinate antiporter"
                     /protein_id="HFO5818830.1"
                     /translation="MKPSTEWWRYLAPLAVIAIIALIPVPAGLESHTWLYFAVFTGVI
                     VGLILEPVPGAVVAMVGISIIAILSPWLLFSPEQLAQPGFKFTAKSLSWAVSGFSNSV
                     IWLIFAAFMFGTGYEKTGLGRRIALILVKKMGHRTLFLGYAVMFSELILAPVTPSNSA
                     RGAGIIYPIIRNLPPLYQSQPNDSSSRSIGSYIMWMGIVADCVTSAIFLTAMAPNLLL
                     IGLMKSASHATLSWGDWFLGMLPLSILLVLLVPWLAYVLYPPVLKSGDQVPRWAETEL
                     QAMGPLCSREKRMLGLMVGALVLWIFGGDYIDAAMVGYSVVALMLLLRIISWDDIVSN
                     KAAWNVFFWLASLITLATGLNNTGFISWFGKLLAGSLSGYSPTIVMVALIVVFYLLRY
                     FFASATAYTSALAPMMIAAALAMPEIPLPVFCLMVGAAIGLGSILTPYATGPSPIYYG
                     SGYLPTVDYWRLGAIFGLIFLVLLVITGLLWMPVVLL"
     gene            complement(16258..17271)
                     /gene="tsaD"
                     /locus_tag="ACHKBL_001952"
     CDS             complement(16258..17271)
                     /gene="tsaD"
                     /locus_tag="ACHKBL_001952"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HFO5818831.1"
                     /translation="MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVP
                     ELASRDHVRKTVPLIQAALKESGLTAKDIDAVAYTAGPGLVGALLVGATVGRSLAFAW
                     NVPAIPVHHMEGHLLAPMLEDNPPEFPFVALLVSGGHTQLISVTGIGQYELLGESIDD
                     AAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFA
                     ANTIRDNGTDDQTRADIARAFEDAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLR
                     AKLAEMMKKRRGEVFYARPEFCTDNGAMIAYAGMVRFKAGATADLGVSVRPRWPLAEL
                     PAA"
     gene            17509..17724
                     /gene="rpsU"
                     /locus_tag="ACHKBL_001953"
     CDS             17509..17724
                     /gene="rpsU"
                     /locus_tag="ACHKBL_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HFO5818832.1"
                     /translation="MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTE
                     RKRAKASAVKRHAKKLARENARRTRLY"
     gene            17835..19580
                     /gene="dnaG"
                     /locus_tag="ACHKBL_001954"
     CDS             17835..19580
                     /gene="dnaG"
                     /locus_tag="ACHKBL_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HFO5818833.1"
                     /translation="MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHN
                     EKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFEAG
                     SGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVATSARQYLEKRGLSHEVIARFAIGF
                     APPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSYDRFRERVMFPIRDKRGRVIGF
                     GGRVLGNDTPKYLNSPETDIFHKGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQY
                     GINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQ
                     LRFMFLPDGEDPDTLVRKEGKQAFEARMEQAIPLSAFLFNSLMPQVDLSTPDGRARLS
                     TLALPLISQVPGETLRIYLRQELGNKLGILDDNQLERLMPKAAESGVSRPVPQLKRTT
                     MRILIGLLVQNPELATLVPPLENLDENKLPGLGLFRELVNTCLSQPGLTTGQLLEHYR
                     GTNNAATLEKLSMWDDIADKNIAEQTFTDSLNHMFDSLLELRQEELIARERTHGLSNE
                     ERLELWTLNQELAKK"
     gene            19775..21616
                     /gene="rpoD"
                     /locus_tag="ACHKBL_001955"
     CDS             19775..21616
                     /gene="rpoD"
                     /locus_tag="ACHKBL_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HFO5818834.1"
                     /translation="MEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDI
                     IQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYM
                     REMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSD
                     LITGFVDPNAEEDLAPTATHVGSELSQEDLDDDEDEDEEDGDDDSADDDNSIDPELAR
                     EKFAELRAQYVVTRDTIKAKGRSHAAAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVM
                     MDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVS
                     EEVHRALQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAK
                     KYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTI
                     RIPVHMIETINKLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISME
                     TPIGDDEDSHLGDFIEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMRFGID
                     MNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD"
     gene            complement(21779..22774)
                     /locus_tag="ACHKBL_001956"
     CDS             complement(21779..22774)
                     /locus_tag="ACHKBL_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO5818835.1"
                     /translation="MSIKKLADGKYCVDVRPAGSEGRRFRRRFPTRGEAAMYERHVLT
                     HYHDKDWVEKPIERKALSDLLELWWVYHGKNHRYGAMNRVRIEAVLRDMQELGINRSD
                     HLTRKNIIRYRLELMNRGIKQSTVNRYCAMMSGFFEKLIDVEEFVGDNPFHEIRKLTI
                     NQPEMAYLAHDDIPRLTALLSGDNLKAVLLSLATGGRWGEVANIKGEHIIGGKVIFME
                     TKNGSRRVIPIAKDLESLIKVKATGRLMNPSYAIVRSAIRTVRPDLPVGQSVHVLRHT
                     FATHFMINGGNIVTLQRILGHSTIQQTMTYAHFAPDYLQDAVRFNPVAELSRFCP"
     gene            complement(22841..23134)
                     /locus_tag="ACHKBL_001957"
     CDS             complement(22841..23134)
                     /locus_tag="ACHKBL_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001519230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO5818836.1"
                     /translation="MDIPQKIRVMRKSEDLTQREFADMIGISYSALTSYEYGRNLPGL
                     EITIKLFKHPRFSKYRDWFLFDEVEPKAGQVVPALAHIGQSEAESSHSGKKTG"
     gene            23250..23675
                     /locus_tag="ACHKBL_001958"
     CDS             23250..23675
                     /locus_tag="ACHKBL_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cox family DNA-binding protein"
                     /protein_id="HFO5818837.1"
                     /translation="MQESTQNAPESLCGTRCDHQSEISEAEHLKTGRKAPERQEIRLS
                     QNPSHLLSKEGFAMYIGKTVDAIVSMAKAGKLPAVYMADPLKPGGNAELYINRKAWDE
                     ACDRLTENAPQEWHEWENRLFLFKPAAGSRNKRISKKAA"
     gene            23989..24324
                     /locus_tag="ACHKBL_001959"
     CDS             23989..24324
                     /locus_tag="ACHKBL_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000194242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4761 family protein"
                     /protein_id="HFO5818838.1"
                     /translation="MTQRRNFRTGTERHAARFSTSASRSNTHYSLQETHATPDGHPVK
                     QTGKHTWLINDAGIVIHRGARNPITGNTCYSLTRGDEQFGQDFTLHEALRTADRLISG
                     RSFQHPWGN"
     gene            24328..24582
                     /locus_tag="ACHKBL_001960"
     CDS             24328..24582
                     /locus_tag="ACHKBL_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000246597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5818839.1"
                     /translation="MATTTKDMAEIASAYTIIIHRLIANNARNALNTAKPLWETKKDI
                     ISALQGLQKCADLAGDYAAYMAIEDTIEKIESGKSLSDFV"
     gene            24603..>24835
                     /locus_tag="ACHKBL_001961"
     CDS             24603..>24835
                     /locus_tag="ACHKBL_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4754 family protein"
                     /protein_id="HFO5818840.1"
                     /translation="MDKEYKVLINKALERFNFRLSMSGVAAEHAARDSLTRAIRSLYD
                     AAFYADDLDALNELSELVRDAECGVHIEPYALG"
BASE COUNT         6176 a         6734 c         6259 g         5666 t
ORIGIN      
        1 gcgattattg ccgtatgcat tctgtttatt attggaatta ttttcgccag gctctatcgt
       61 cgcgcttcgg cagagcaagc ttttgttcgt actggtttag gtgggcaaaa agtggtaatg
      121 agcggtggcg caatcgtgat gccgatcttt catgaaataa tccccatcaa tatgaatact
      181 ctgaagctgg aagtcagccg ctcaaccatt gatagcctga ttacgaaaga tcgtatgcgc
      241 gtcgatgtcg ttgtcgcttt ctttgtgcgg gtaaaaccgt cagtggaagg tattgccacc
      301 gccgcccaga cgcttgggca acgcaccctg tcgcctgaag acttacgtat gttggttgaa
      361 gataaatttg tcgatgccct ccgtgcaaca gctgcacaaa tgaccatgca tgagttacag
      421 gatacccgcg agaacttcgt gcagggcgta caaaataccg tagctgaaga tctgtcgaaa
      481 aacggcctgg aactggaaag cgtttcactt accaacttta accagacgtc gaaagaacat
      541 ttcaatccta acaacgcctt tgacgccgaa ggtttaacta agctgacgca ggagacggag
      601 cgccgtcgtc gcgaacgtaa cgaagttgaa caggatgtag aagttgcggt gcgtgagaaa
      661 aaccgtgatg cgctttcgcg gaagctggag attgaacagc aagaagcgtt tatgacgctt
      721 gagcaggagc agcaggttaa aacccgtacc gctgagcaga atgcgaaaat tgcggctttt
      781 gaggccgagc gtcgtcggga agcagagcag acgcgaattc tggctgaacg acagattcag
      841 gaaacggaaa tcgaccgcga acaggccgtc cgttccagaa aagttgaagc tgaacgtgaa
      901 gttcgcatta aagagatcga acagcagcag gtcaccgaaa tcgccaacca gacgaaatcg
      961 atcgcgattg ccgccaaatc ggaacagcag tcacaagcgg aagcgcgtgc caaccttgca
     1021 cttgcagaag cggtaagcgc ccagcaaaac gtagaaacca ctcgccagac tgccgaagcc
     1081 gatcgtgcta aacaagtggc gttgatcgcc gcagcgcagg atgcagaaac caaagcggtt
     1141 gaactgaccg tgcgggcgaa agcagaaaaa gaagccgcag aaatgcaggc ggcggctatc
     1201 gttgagttag ccgaagctac acgtaaaaag ggtctggcgg aagcagaagc acaacgtgcg
     1261 ctgaacgatg ctatcaacgt actttctgat gaacaaacca gccttaaatt caaactggca
     1321 ttattgcagg cgctgcctgc ggtaattgag aaatccgtgg aaccaatgaa gtctatcgac
     1381 ggcatcaaaa ttattcaggt cgatgggctg aatcgtggtg gcgctacggg tgatgcaaac
     1441 acgggtaatg tggggggcgg aaacctggca gaacaagcgt tatcagccgc actctcttac
     1501 cgtacacagg caccgctgat tgactccttg ctcaatgaaa ttggcgtttc aggcggctca
     1561 ctggcggcat tgacttcatc cttaagctca acaactcccg tcgaagaaaa agcagagtaa
     1621 accctcttct atgaataatc cctggcccaa aaagccgggg attttttata tctgcgttcc
     1681 gctaaaaggt gcaaatgatc aagctgttgc agcattttgc gtgactgctc ggggaaggca
     1741 aaactgcctc tgggaaagcg ttgcgcgggg tccggcgctc atcaacaatc ggggagtcag
     1801 caaggggctg aaacgggaaa gcccctcccg aggaaggggc cttgtataag gaaagggtta
     1861 tgatgaagct cgtcatcata ctgattgtgt tgttactcgt aagtttcgca gcttattaac
     1921 agccaatcag aggggggaga aatcctccct cacccttgtt cctttacttt aggttgaaaa
     1981 aaaatcagcg tcaataggcc cgcctgctac aaagcgagat ctgtgaaccg ctttccggtt
     2041 agcctttttt atcctgttgg atcttcttga tgatgttggt cgtcgagcaa ccgtcttcaa
     2101 agttgagcac cagcacttca ccaccgttgg cccagacttc tttactcccg gcaatctctt
     2161 ctggtttata gtcgccgcct ttcaccagta gatctggcaa gataccggcg atcaagcgct
     2221 gcggcgtgtc ctcttcaaac gacaccaccc agtcgaccgc ttccagtgcg cccagcacaa
     2281 tcatacgctg ttcgagctgg ttaacggggc gggaatcccc tttcagccgt ttggtggagg
     2341 catcgctgtt cacagcgaca atcaaacggt cacccagttt acgggcattt gccagataag
     2401 agacgtgccc agcgtgcagg atgtcaaaga caccgttggt catcaccact ttttcaccgc
     2461 gtttacgcgc tgccgctaca gcaagcttca gttcctcttc ggtcatcaca ccaaagccgg
     2521 tatctgcacg tccacgtaca gcgttttcca gctcgatcgg cgaaaccgtg gatgtcccca
     2581 gtttgccgac caccacacca gccgccgcat tggcaaagaa gcaggcttct tccagtgaat
     2641 tacccgctgc cagcgttgcc gccaggacgc caatcaccgt gtcgcccgca ccggtaacgt
     2701 catacacttc ctgcgcctgg gttggcatat gcagtggcgc tttacccggt tgcagcagcg
     2761 acataccctg ttcggaacgg gtcactaaca gagccgagag ttcgtaatcg gcaatcagtt
     2821 tcatgccgcg ctcaacaatc tcttcttcgg tcttacattt accgacaaca gcttcaaatt
     2881 ccgagagatt cggcgttaac agcgtagcgc cgcggtagcg ctcaaaatcg gtaccttttg
     2941 gatcaatcag caccggaaca cccgctttac gcgccagttg gatcatctgc tgtacgcttg
     3001 ccagcgcacc tttggcgtaa tcagaaagca ccagcgcgcc aatcgaactc agtgcctgat
     3061 taatccgctc gtgcagcggc tgcggatcaa ccccttcgaa accttcttcg aaatccagac
     3121 ggatcagttg ttggttgcgg gaaagtaccc gtaatttggt gatggtcgga tgcgtcggta
     3181 cagaaacaaa gtcgcatttg acgttgacgt cggccagaga tttactgagc gcgcgcgccg
     3241 catcgtcaat gcccgtcaac ccgaccaggc gtgcattagc accgagagaa gcgatattca
     3301 tcgccacgtt agccgcgccg cccggacgtt cttcgatggt attcacttta accacgggca
     3361 ccggcgcttc cggcgagata cggctggtgg gaccgtacca gtaacgatcc agcatcacat
     3421 caccaaccac catcactcct gcacgttcaa actctggcag cgttactttc attcctgtct
     3481 cctgagagat tcaaaatttg cgcgcgataa taccatactt cattcttcca ccagccactt
     3541 ctgccagctt gcccgcacca gttcacgctc tgcggtgaag caatcctccg acacatgacc
     3601 cggcaattcc tgtaatgcca gatgatgaag ttcatcgcgc agcgtcgtgt aagcacgggt
     3661 cagcgccatc gcttcctgct cttccataat gtcgttttgc gccagtagtt ccagaatgcg
     3721 cacgttgtct gaccagcgcg ttaacttcgg tttttcgtga gcgtagcgca acaccagata
     3781 ttgagtaata aattcgatat cggtaattcc gccttcatcg gctttgatat caaagcgatc
     3841 gcgatgttta ttgccgagat gggcacgcat tttctcgcgc atttcccgca cttctgtttg
     3901 cagggttttg ccttcgcgcg gcagcgtcat aatctcccgg cgtactgcgt caaagtgcgc
     3961 ggtgagctgc ggatcgccgt acactacacg cgcacgcacc agcgcctgat gctcccacgt
     4021 ccaggcctcg tttttctgat aatcagcaaa tgcttctgcg gatgtcacca gcattcccgc
     4081 cgccccggac ggacgcagtc gtgcatccac ttcatacaaa atgccggaag aggttcgcgt
     4141 actgaacaga tgcataatgc gttgcgccag acgcagataa aactgtcgcc cgtcgatttc
     4201 ccgctcgccg tcagtcatcg catccattgg gcaatcatgg aggaagatca ggtcaagatc
     4261 ggaactgtag cctaactccc agccacccag cttgccgtag ccgaccaccg caaaaccacg
     4321 cccttcgcgt tcgttcaggt gatttggctt accgtagcgg gcaaccattt gaacccacgc
     4381 ctgctgaacg acggcatcaa tcatggcttc tgccagccag gttaagtgat cgctcacttt
     4441 cattaccggt agcgtaccgg cgatatccgc tgccgcaatg cgtaacagct gcgcctgttt
     4501 gaactgacgc agcgcctcaa gctgttgttc ttcgtcatct tccggcacgc gcagcaaata
     4561 ctggcgcagt tcatcacggt aggcatccgt cgccgttggt tggtagaggg tatttggatc
     4621 gagcaattca tccagcaata atggataacg cgctagctgg ctggcaatca tcggcgatgc
     4681 agcacacaga gaaatcaaat gtttaagcgc cgcagggaat tcactaagca gctcaagata
     4741 agtggtgcga gtaacaatcc ccaccagcaa ggcggtaatg cgcgacagcg tgacagcagc
     4801 gtcttcacgc gcacagacat cacttagcag atgtggcatc agatggtcga gcacctgacg
     4861 tcctcgcggc ccgatggtgc gcttatccag ctctttgcgg aaatcagcaa tcagcattag
     4921 cacctgtttg cgatcatcct cgctaagatg cgccagcact ggcgtggtgt catcttcctg
     4981 caacgcatcc tgccacagct cacgccactg ttccgacagc gactcttcct gagtttcact
     5041 ttcgtcgtcg ccaatcaatt cattaaacac ccggcgcaca ttggccatat gtgcggtcag
     5101 caccccggtc aattgcggcc agtcagcaaa gtccatcgcc cacgccagcc gcgcacggtt
     5161 aagctcatca aaaggaagcg tctgggtttg ttcgtcgtta atgctttgca gcaggttttc
     5221 cagacgccgc aggaacagat acgccactcg caattgttca gcatcgtttt cagaaagcag
     5281 atgcagcgcg gcaatggcgc tgagcgttgg cagtaaagag cgcgattgca gcgacggttc
     5341 gcgcccgccg cgaatgagct ggaacacctg gacgataaat tcaatttcgc gaatgccgcc
     5401 tgcgccaagt ttgatgttgt cggtcaaacc gcgtcgacgc acttcacggg caatcattcc
     5461 tttcatgttg cgcagcgact gaatcacgct gaaatcgatg taacgacgga aaacaaacgg
     5521 acgcagcatc gcacgcaact cgttagcata gacgccgtcg ctatccccca taatccgcgc
     5581 cttgaccatc gcgtaacgct cccagtcgcg cccctgctcc tggtaataat cttccagcgc
     5641 cgcaaaactc agcaccagcg ggccactttc accaaacgga cgcagccgca tatccacgcg
     5701 atagacgaaa ccatcctgcg ttggttgatc cagcacttta atcagccgct gccccatgcg
     5761 ggtaaaaaac tgcgcgttat ccagttcccg gcgtccgccc tgggtacaac catgttccgg
     5821 ccaggcaaaa atcagatcga tatctgagga gaaattcagt tccccaccgc ccagcttacc
     5881 catgcctaaa atcagcagcg gttgcgcttc gccctgcgca ttgcacggcg ttccccattc
     5941 gcggcagcag gcgtcataca gccagtcacg cgccgcaaca atcagcgtct ccgccagatg
     6001 gctgagctgc tgcaatatgc tctcttcagt aaccagtgcc agcgtttgcg cccaggcgat
     6061 gcgcaccata atgcgccgcc ggaatagccg cagctcgcgc attaacccgg cttcgtcact
     6121 cacattactg agcgcctcct gcaaccatgc tgcgtaatgc tgccattcgt cggcctgcgg
     6181 cggttggctt tccagttccg tcagccactc tggatgcgca attatgctgt cctgcagaaa
     6241 atcactgaaa gtaagtactg acttcgcctg tgcgctaagt gattcctcgg ctaaaggctc
     6301 tggcagccgc tcaacaacgg tctgccagta ctgctgtaac ggtgaagaga gcggcttcat
     6361 aagcgatttt atccttgcct gattaacgtt ttccgctgtg caaccagaac ggttcctgat
     6421 tgtttgcctc attacggaaa tgttcaattt caatgcgttg cccggtcgca atagcgtgac
     6481 gcagcccctg ccagttctcc agccaggctt gcgcgacgac aggatcgtag taacccgcca
     6541 gcaacaggat tgagtcaata tcacgcgtca ggcgtggcaa ctggtcacgg tagcgatcgc
     6601 ccaacggctg gcagaaaacg cttttcagtt cagcggcatg gcgggaaaga tggatatcgg
     6661 caaagcgttt gaaggaatcg ctcattttac tctgcgcttt ggcatctaaa aacggctgcc
     6721 aggctttgct taccagccat tcggtcaatg ccagcttcgc cattgccgtt tcggtagagt
     6781 agaccgccgt cacggcagaa accgctgaag cgatggtcgc ttcgcattga gtcaataaat
     6841 cacgtaagtg agtgctcgct ttacgcggca caatgccacc gaacagcatc agcgtatgac
     6901 ggaccaggcc aatggctgcc agcacctgct ctttcgccgc atcattgccg cgtacccata
     6961 gttcttcatg atactgccat tgcgctaacg ccagctcgag cgccgcttcc agcccctgtt
     7021 ccacatcagc ttttgccgca acatgtaaaa tggtggtcgg tttgatttca cgcgccggat
     7081 tgccctgcgc cagatgatag ccacgcgccg ctttgctcag actgccctgg cgtaatccgg
     7141 tttgcgatac cagttggttc gccagtttca gcaccgcgcg cgtgtcgccg ctaagcagtt
     7201 ccagttccag ctcgcagata ggttcagcaa attcacccgc tttcacttcc ccctggtcga
     7261 gggcgatttc aatttggcta ccatcaacct ctaccagcca cttttcgcga taaaaatcgg
     7321 tgctaaacag cggctgcacg cgggaagcga gatcggtagg caattcgccg ttcggccaga
     7381 cttccgtcgg taactgcgcc aggtcgagcg tcggttcgct caacgccacg ttatattccg
     7441 ggcgctggtg taagccgcct gtcactcttc ctgcaacttt catggtcatc tcatagcgac
     7501 cgttttcgcc acgaatacgc aagcccatgt cgtgcccacg cagccagtta tccggcgttt
     7561 cgtagtaaat attcagcaac tgcacggggt catggtgctc gccgcccagc gtattgagat
     7621 ggtcacgcaa cgcctcaacg gcactgtgat taacaataaa ctttaattcg atttcctgag
     7681 ccatagcctt tacttatcgg ttatgtcaca taagtgacga tgaacggcga acttaatgcg
     7741 atcttttttg tcagtagata gtattttgcg ccaaattgcc atgcaacggg caatttgacg
     7801 ggcgtaaaag tttgaagcag tggcaagacc tgacacagag gattccgatt gatgtcgaat
     7861 gctttgcgta gcgacactga tgccactact atcgttccac tttccatgac aataacgaca
     7921 gcctgatgcc aaaattacgc ctgatcggat taactttact cgcacttagc gccactgccg
     7981 tctcacacgc tgaagaaacg cgctatgttt ctgatgaact gaatacctgg gtccgtagcg
     8041 gtccgggaga tcattatcgc ctcgtgggca cggttaacgc cggcgaggaa gtgaccttat
     8101 tacaaactga cgccaacacc aattatgccc aggtgaaaga cagctctggc cgtaccgcct
     8161 ggatcccgtt gaaacaactt agcacagagc caagcctgcg ctcccgtgtg ccagatctgg
     8221 aaaatcaggt caaaaccctg accgataaac tcaccaatat cgataacacc tggaaccaac
     8281 gcaccgcaga aatgcagcaa aaagtggcgc agagcgacag cgtgatcaac gggttaaaag
     8341 aagaaaatca gaaactgaaa aacgagctga ttgtcgcgca gaaaaaggtc gatgccgcca
     8401 gcgtacagct ggatgacaaa cagcgcacca tcatcatgca gtggtttatg tatggcggtg
     8461 gcgtgctggg gcttggcttg ctgcttggtc tggtactgcc gcacctgatc ccaagccgca
     8521 aacgcaaaga tcgctggatg aactaaatcg ccttcttcgt tgcactgtct tattattagg
     8581 aattgtgtat ttgacgaagg gatgatggcg tgaagattta tctggtcggt ggtgctgttc
     8641 gggatgcatt gttagggcta ccggtcaaag acagagattg ggtggtggtc ggcagtacgc
     8701 cacaggagat gctcgacgcg ggctaccagc aggtaggccg cgattttcct gtgtttctgc
     8761 atccgcaaac gcatgaagag tatgcactgg cacgtaccga acggaaatct ggttccggtt
     8821 acaccggttt tacctgctat gccgcaccgg atgtcacgct ggaagatgat cttaagcgtc
     8881 gcgatctgac cattaatgcg ctggcccagg acgataacgg cgagattatc gacccgtaca
     8941 acggtctggg cgatctgcaa aatcgtctgt tgcgccatgt ttcccccgct tttggcgaag
     9001 atccgttacg cgtgttgcgc gtagcgcgtt ttgctgcgcg ttatgcccac ctcagtttcc
     9061 gtattgccga tgaaactctg gcgctgatgc gcgagatgac ccatgcgggt gaactggaac
     9121 acctgacgcc tgaacgggta tggaaagaga cggaaaacgc cctcaccacc cgcaatcccc
     9181 aggtgttctt ccaggtactg cgcgattgcg gcgcactgcg cgttttattc ccggaaattg
     9241 acgccctgtt tggcgttccg gcccctgcca ggtggcatcc ggaaatcgac acgggtattc
     9301 ataccttaat gacgctctca atggcggcga tgctgagtcc gcaggtcgat gtccgtttcg
     9361 caacgttatg ccacgatctc ggtaaaggac tgacgccacc agaactttgg ccgcgtcatc
     9421 atggtcatgg cccggcgggt gtgaagttag tggaacaact atgccagcgt ctgcgcgtgc
     9481 caaatgaaat tcgcgattta gccagactgg tggctgagtt tcacgatctc atccacacct
     9541 tcccaatgct gaacccaaaa accatcgtca aactatttga ttccatcgac gcctggcgta
     9601 aaccgcagcg tgtcgagcaa ctggcgctga ccagcgaggc tgatgtgcgc ggcagaaccg
     9661 gttttgaatc agccgactac ccgcaaggcc gctggttgcg cgaagcctgg gaagtggcac
     9721 agtcagtgcc gacaaaagcc gtcgttgaag cgggatttaa aggtgtggag attcgcgagg
     9781 agctgacccg acggcggatt gcggcggtgg ctggctggaa ggaacagcgt tgcccaaaac
     9841 ctgagtgagt gttggatatt ttgccggatg cgacgtaagc gtcttatccg gctacatgtc
     9901 aacgacagtt gtaggcctga taagacgcgc gagcgtcgca tcaggcgttg attgccggat
     9961 gcggcgtgaa cgccttatcc ggcctacgca atctgataag acgcgtcagc atcatatccg
    10021 acactgttct tcacatcaaa agaacacaac gtacaccgca gccgccacaa taaagcgata
    10081 aatggcgaac gggataaacg aaatgcgctt aatcaattgc aggaaggttt taatcgctat
    10141 cagcgccacc acaaaagcgg tgataaaccc aacggcaaac atcgggatat cgccggtagt
    10201 caggaagccc cagcttttgt agagatcgag cgccgttgcg cccatcatca tcggcaccgc
    10261 cagcaggaac gagaactcgg aagcagcgta acggctcacc cccatcagca tcccacctga
    10321 aatggtcgcc ccggaacggg agaaacccgg ccacagcgcc agacactgga aacagccaat
    10381 cataaacgcc tgacgatagg tcatatcatc aagacccggc gcacgcggct ctttcggctt
    10441 caggcattcg gcggcaatca gcaacagacc gccaacgacc agtgcataca tcacatttat
    10501 cgggttaaac aatgatttaa tcgtgtcgtg gaacaacagc cccagtacca ccgccggaat
    10561 catccccagc aaaatgtgga tcagcgttaa acggcctttg ctttctcctt cgtgctgcaa
    10621 cgggcgacca aagtgaatac caataaggcc aaacagacgt cgccagaaca tgacaaccac
    10681 tgccagaatt gatcctaact ggatcacgac ttcaaaggtt ttcgccgtgt cgccctcaaa
    10741 ccccaacaag tgaccgacaa taatcatatg gcccgtgctg gataccggca gaaattctgt
    10801 caatccttcg accacaccca atattgccgc tatcagcagc gagtgcatat cgctcatcaa
    10861 taaaccccta aattataaaa atgtaacgct tgtccgaact actgcagtat gaccaggtta
    10921 taaccgtttg gtttaacagc tgtaaaatta attattttct ttcagattat tgccacgctc
    10981 aatgattacg ccaacattcg ccgcccgcgc cactgcgcct ggcttgctga gtttgatacg
    11041 cacccacggc gagttgaagc gtgctaacag caactccgcc acctcttcag ccacacgttc
    11101 caccagcgca aaacgcgccc cctcgacgtg gctgactacc gtttctgcaa tgtcagcgta
    11161 actgaggcaa tccgccacat catcactttt cgccgcttta cggttatccc acgccatttc
    11221 gatatcgaac actaacttct gttcgatggt ctgttcccag tcgtaaacac caatagtggt
    11281 gattaccgaa agttgctcta taaatacaat atccatcacg tcctgcctgc tttttggcta
    11341 accggatacc acttccggcg aaatgtgcgt attatccaca gattcatcgt tgaacacgaa
    11401 ttttcaaaac ggaacagctt atgagtgcaa tcgcgcctgg aatgatcctc atcgcgtacc
    11461 tctgcggctc catttccagt gccattctgg tttgccgctt gtgtgggctg cccgatccgc
    11521 gaaccagcgg ctccggcaat ccaggcgcaa ccaatgtgtt acgtatcggt ggcaagggag
    11581 cagccgtagc agtactgatt ttcgacgttc tgaaaggaat gttgcccgtc tggggcgcgt
    11641 atgaattagg tgtcagcccc ttctggctag gcttaattgc catcgccgcc tgtcttggac
    11701 acatctggcc cgttttcttc ggatttaaag gaggaaaagg cgttgctacc gcttttggtg
    11761 ccatcgcacc cattggctgg gatctcaccg gagtaatggc gggaacctgg ttactgaccg
    11821 tgctattgag cggatattcg tcgctggggg cgattgtcag tgcactgatt gctccgtttt
    11881 atgtctggtg gtttaagcca caattcacct tcccggtttc gatgctctct tgcctgatcc
    11941 tgctgcgcca tcatgacaac atccagcgtc tgtggcgtcg tcaggagaca aaaatctgga
    12001 cgaaattcaa aagaaagcgc gaaaaggatc ccgagtgatt tctggtggag ctacataacc
    12061 tgatagcctt cgtcgggctt gcccagccgt tgctggcacc atgccgccag aaattccacg
    12121 cagacacgta atttcatgct gcgatagagc ggctcccggt aaacagccca gatattggcg
    12181 ctttgtgcat actccggcaa tactcgcacc agtttgccac tctccagaaa cggcaacaca
    12241 tcccactcgg aacgcaacat aatccctttg ccctccagcg cccattgcaa cactatctcg
    12301 ccgctattgg aggaaagatg cccgcttacc ttcaccgatt ttttctcctg accgttcccc
    12361 aactcccata ttccgtgggt catatcgcgc tctttggtca ccaggcagtc atgacgactt
    12421 aattcctgta aggattgtgg ctgcggatat ttttgcagat actcaggtgc tgcgcataat
    12481 attcttttat tttttgtcaa aagatgcgca atataataat caggaattgc gtcattaata
    12541 cgaatatcca gatcaatatt atcctgcact aaatcaattt gccgatcgaa caattcaaaa
    12601 tgcacctgta actcaggata attgcgcatc agttcggtaa tggctggagc aatatggctg
    12661 cgcccaaaac caaaactgca accaatgcgg atcatcccct ccgggcgcgt tttgatttgc
    12721 gtgacgtcat cgaccagtcg ctgatattgg gtgaggattt ccagcgcatg ttcgtaacaa
    12781 cgttggccac tttcagtcag cgccacgccg cgagcggagc ggttcagcag agttgttgcc
    12841 agcgtatttt caaggatttg gatccgcttg gtgacaaatg caggcgtttg cccaagcgtc
    12901 gccgctgcag cactaaaact cccgctatgg acgatttcca ccagtacctg taagtctttg
    12961 gctaaaggcc agctatttag cattgttaaa tatccgcagt tttgataaac gcagtgtatt
    13021 tcgcaaaaca ttaccattcc ctgctctggc gcaatattcg ttcttatttt cgcgttgatt
    13081 cacgaactgt taattacata ttcacggtgg caaaaaatat aaaaccacat ttttagtggt
    13141 agtttgtggc ggtgaatttt tccagacaaa tacataaact ggagttgcca tgatgagcga
    13201 aagtaataag caacaggcag tgaataagtt gacggagatt gtcgctaact ttaccgccat
    13261 gatttctacc cgaatgcctg atgacgtggt ggataaacta aaacagctaa aggatgccga
    13321 aacatcgtcg atgggaaaaa tcatctacca cacgatgttc gataacatgc aaaaagcgat
    13381 cgacctgaat cgtcctgcct gtcaggacac cggcgaaatc atgttttttg ttaaggtcgg
    13441 ttcccgtttc ccactgcttg gcgagctgca aagcatcctc aaacaagccg tggaagaggc
    13501 aaccgtcaaa gcgccactgc gtcacaatgc ggtagaaatt tttgacgaag tgaacaccgg
    13561 caaaaatacc ggcagcggcg taccgtgggt cacctgggat atcgtccccg acggtgacga
    13621 tgcggaaatc gaagtttaca tggcaggcgg cggctgcaca ctacctggtc gttcgaaagt
    13681 gttaatgccg tcggaaggat acgaaggcgt ggtgaaattc gtcttcgaaa atatctccac
    13741 cctcgccgta aacgcctgtc caccggtact ggtaggcgtg ggcatcgcca cctcggtgga
    13801 aaccgccgct gtgctctcac gtaaagccat tttacgccca attggcagcc gccaccccaa
    13861 tccgaaagcg gcagagctgg agctacgcct ggaagaagga ctcaaccgtc tggggattgg
    13921 tccacaaggg ctgactggca acagttcagt gatgggcgta catatcgaat ctgccgcccg
    13981 ccatccgtca accatcggcg ttgctgtttc tacaggctgc tgggcgcatc gtcgcggcac
    14041 actgctggtt catgccgatc tcacctttga aaatctgtct cacacccgga gcgcgttatg
    14101 aaaaagatcc tgacaacccc gatcaaagct gaagatctgc aagatattcg cgtcggcgat
    14161 gtgatctacc tgaccggtac gctggtgacc tgccgtgacg tttgtcaccg tcgcttgatc
    14221 gaactcaaac gtccgatccc ttacgatctc aacggcaaag cgattttcca cgctggcccc
    14281 atcgtgcgca aaaacggcga caaatgggag atggtatccg tcggcccgac aaccagtatg
    14341 cgtatggaaa gttttgaacg tgaatttatc gaacagaccg gcgtgaaact ggtggtcggc
    14401 aaaggcggaa tggggccgct gaccgaagaa ggctgccaga aattcaaggc gctgcatgtg
    14461 attttcccgg caggctgcgc agtactggcg gcaacccagg tggaagagat tgaagaagtg
    14521 cactggacag aactcgggat gccggagtcg ctgtgggtct gccgggtcaa agagttcggt
    14581 ccgctgattg tttctattga tacccacggc aacaacctga tagccgaaaa caaaaagctg
    14641 ttcgccgaac gccgcgatcc catcgtggaa gagatttgcg agcacgtcca ctacatcaaa
    14701 taaccctccc ggagaggctc accccctctc ctttttcgca ggcataacac gatgaaacct
    14761 tccactgaat ggtggcgata ccttgcgccg ctggcggtca tcgccattat tgctctaatt
    14821 ccggttcccg cagggctgga gagtcatacc tggctctact ttgccgtttt tactggcgtg
    14881 atcgttggac tgatcctcga acccgtgccg ggtgccgtgg tggcgatggt gggtatctcc
    14941 attatcgcca ttctctcgcc gtggctgctg tttagcccgg agcagctcgc ccagccaggt
    15001 tttaagttca ccgccaaatc cctctcctgg gcggtttctg gtttttctaa ttcggttatc
    15061 tggctgattt tcgccgcctt tatgtttggc acaggctatg aaaaaaccgg gcttggacgc
    15121 cgcattgcgc tgattctggt gaaaaagatg ggacatcgca cgctgtttct cggctatgcg
    15181 gtgatgttct ccgagctgat cctggcacct gtaacaccgt ccaactcggc gcgcggtgcg
    15241 gggattatct atcccatcat ccgtaacctg ccaccgctct atcaatcaca accaaacgac
    15301 agcagttcgc gcagcattgg ctcgtacatc atgtggatgg ggattgttgc cgactgcgtg
    15361 accagcgcca ttttcctgac ggcgatggca cctaacttgc tgttaattgg cctgatgaaa
    15421 agcgcatctc acgccacact gagttggggc gactggttcc tcgggatgtt gccgctcagt
    15481 attttactgg ttctgctggt tccctggctg gcttacgtgc tgtacccgcc ggtactgaag
    15541 tctggtgatc aggtgccgcg ctgggcagag acggaactgc aggcaatggg cccgctctgt
    15601 tcgcgtgaaa aacggatgct ggggctgatg gtaggcgcgc tggtgctgtg gattttcggc
    15661 ggtgattata tcgatgccgc gatggtcggt tacagcgtgg tggcgctgat gctgcttctg
    15721 cgcattatca gttgggacga cattgtcagt aataaagccg cgtggaacgt tttcttctgg
    15781 ctggcctcgc ttatcaccct cgctaccgga ctcaacaaca ccggttttat tagctggttt
    15841 ggcaaactgt tagcaggcag cttaagcggt tattcgccaa cgatagtgat ggtggcgttg
    15901 attgtggtgt tttatctact gcgctacttt ttcgccagcg ccacggcgta tacctccgct
    15961 ctcgcgccga tgatgattgc cgccgcgctg gcgatgccgg aaatcccgct gccggtgttc
    16021 tgcctgatgg ttggcgcggc aattggtctg gggagcattc ttacgccata cgccaccgga
    16081 cccagtccga tttactacgg tagtggttat ctgccaacgg tggattactg gcgactgggg
    16141 gcaatttttg ggctgatatt cctcgtgttg ctggtgatta ccggcttact gtggatgccc
    16201 gtggtgttgc tttaagtgag gacggtgccg gaagtgactc tccggcacct aagagtttta
    16261 cgcggccggt aactctgcca gcggccagcg cggacgcacg ctaacgccaa gatccgccgt
    16321 cgcgcctgct ttaaaccgca ccattccggc ataggcgatc atcgcgccgt tatcagtaca
    16381 aaattccgga cgcgcgtaga acacttcgcc acggcgtttt ttcatcattt ccgccagctt
    16441 cgcccgtaac gtgcggttag cactcacgcc acctgccatt accagtcgct taaagcccgt
    16501 ctgatccagc gctcgcttac acttaatcat caacgtatcg accaccgcat cttcaaaggc
    16561 gcgggcgata tcagcacgcg tctggtcgtc ggtgccgtta tcacgaatgg tatttgccgc
    16621 gaaggttttc agaccagaaa agctgaaatc cagccctgga cggtcggtca tcgggcgcgg
    16681 gaagacaaag cgcccggctg taccctgagc cgccattttc gacagtaatg gcccgccagg
    16741 ataatccagc cccagcagct tcgcggtttt atcaaacgct tcaccagccg catcatcgat
    16801 agactcgccg agcagctcgt actgaccaat gccagtcacg ctgattaact gcgtatgacc
    16861 gccggaaacc agcagcgcca caaacggaaa ttccggtggg ttatcttcca gcatcggcgc
    16921 taacagatgc ccttccatat ggtgtacagg gatcgccgga acgttccagg caaacgccag
    16981 agaacgcccc acggtcgcgc caaccagcag tgcgccgact aatccagggc ctgcggtata
    17041 ggccacagca tcaatgtctt ttgccgttaa accagactcc tttagcgccg cctggatcaa
    17101 cggtacggtt ttacgcacat gatcgcggga ggccagctca ggcactacgc cgccgtagtc
    17161 agcgtgcaat ttcacctgac tatacaattg gttggctaac aaaccttttt catcgtcgta
    17221 aatggcgatg ccggtttcat cgcaggaagt ttcaataccc agtacacgca tgacttgttt
    17281 tacctcgctt tattaccgcg cagtgtagga ccaatgcggg ttgatgtaaa actttgttcg
    17341 cccctggaga aagcctcgtg tatactcctc acccttataa aagtcccttt caaaaaaggc
    17401 cgcggtgctt tacaaagcag cagcaattgc agtaaaattc cgcaccattt tgaaataagc
    17461 tggcgttgat gccagcggca aaccgaatta atcaaaggtg agaggcacat gccggtaatt
    17521 aaagtacgtg aaaacgagcc gttcgacgta gctctgcgtc gcttcaagcg ttcctgcgaa
    17581 aaagcaggtg ttctggcgga agttcgtcgt cgtgagttct atgaaaaacc gactaccgaa
    17641 cgtaagcgcg ctaaagcttc tgcagtgaaa cgtcacgcga agaaactggc tcgcgaaaac
    17701 gcacgccgca ctcgtctgta ctaattcccc gagagcgttg ctctccgatc agaccgagtt
    17761 gtagttgtaa ggccgtgctt ccgaaaggaa tgcgcggctt attttcgttt atgaattgct
    17821 aaaaatcggg gcctatggct ggacgaatcc cacgcgtatt cattaatgat ctgctggcac
    17881 gcactgacat cgtcgatctg atcgatgccc gtgtgaagct gaaaaagcag ggcaagaatt
    17941 tccacgcgtg ttgtccattc cacaacgaga aaaccccgtc attcaccgtt aacggtgaga
    18001 aacagtttta ccactgcttt ggatgtggcg cgcacggcaa cgcgatcgac ttcctgatga
    18061 actacgacaa gcttgagttc gtcgaaacgg tcgaagagct ggcggcaatg cacaatcttg
    18121 aagtgccatt tgaagcaggt agcggcccca gccagatcga gcgccatcaa cgacaaacgc
    18181 tttatcagtt gatggacggt ctgaatacgt tttaccaaca atctttacag caacctgttg
    18241 ccacgtctgc gcgccagtat ctggaaaaac gcggattaag ccacgaggtt atcgcccgct
    18301 ttgcgattgg ttttgccccc cctggctggg acaacgtcct gaagcggttt ggcggcaatc
    18361 cagaaaatcg ccagtcattg attgatgcgg gcatgttggt cactaacgat caggggcgca
    18421 gttacgaccg tttccgcgag cgggtgatgt tccccattcg cgataaacgc ggtcgggtga
    18481 ttggttttgg cgggcgtgtg ctgggcaacg atacacccaa atacctgaac tcgccggaaa
    18541 cggacatttt ccataaaggc cgccagcttt acggtcttta tgaagcgcag caggataacg
    18601 ctgaacccaa tcgtctgctt gtggtcgaag gctatatgga cgtggtggcg ctggcgcaat
    18661 acggcattaa ttacgccgtt gcgtcgttag gtacgtcaac caccgccgat cacatacaac
    18721 tgttgttccg cgcgaccaac aatgtcattt gctgttatga cggcgaccgt gcaggccgcg
    18781 atgccgcctg gcgagcgctg gaaacggcgc tgccttacat gacagacggt cgtcagctac
    18841 gctttatgtt tttgcctgat ggcgaagacc ctgacacgct ggtacgaaaa gaaggtaaac
    18901 aagcgtttga agcgcggatg gagcaggcga taccactctc cgcatttctg tttaacagtc
    18961 tgatgccgca agttgatctg agtacccctg acgggcgcgc acgtttgagt acgctggcac
    19021 tgccactgat atcgcaagtg ccgggcgaaa cgctgcgaat atatcttcgt caggaattag
    19081 gcaacaaatt aggcatactt gatgacaacc agcttgaacg attaatgcca aaagcggcag
    19141 agagcggcgt ttctcgccct gttccacagc taaaacgcac gaccatgcgt atacttatag
    19201 ggttgctggt gcaaaatcca gaattagcga cgttggtccc gccgcttgag aatctggatg
    19261 aaaataagct ccctggactt ggcttattca gagaactggt caacacttgt ctctctcagc
    19321 caggtctgac caccgggcaa cttttagagc actatcgtgg tacaaataat gctgccaccc
    19381 ttgaaaaact gtcgatgtgg gacgatatag cagataagaa tattgctgag caaaccttca
    19441 ccgactcact caaccatatg tttgattcgc tgcttgaact gcgccaggaa gagttaatcg
    19501 ctcgtgagcg cacgcatggt ttaagcaacg aagaacgcct ggagctctgg acattaaacc
    19561 aggagctggc gaaaaagtga tttaacggct taagtgccga agagcgatcg ggaagccccc
    19621 gacagccgca ctgagaggca gcggcaaata tataagtacg ccctcgtaat tatcgttggc
    19681 ggtaaacaac cgttggattt cagcgttaac ggctgaagga catcgggtca atcgcccaac
    19741 accaacctca tgaaataagt gtggataccg tcttatggag caaaacccgc agtcacagct
    19801 gaaacttctt gtcacccgtg gtaaggagca aggctatctg acctatgccg aggtcaatga
    19861 ccatctgccg gaagatatcg tcgattcaga tcagatcgaa gacatcatcc aaatgatcaa
    19921 cgacatgggc attcaggtga tggaagaagc accggatgcc gatgatctga tgctggctga
    19981 aaacaccgcg gacgaagatg ctgccgaagc cgccgcgcag gtgctttcca gcgtggaatc
    20041 tgaaatcggg cgcacgactg acccggtacg catgtacatg cgtgaaatgg gcaccgttga
    20101 actgttgacc cgcgaaggcg aaattgacat cgctaagcgt attgaagacg ggatcaacca
    20161 ggttcaatgc tccgttgctg aatatccgga agcgatcacc tatctgctgg aacagtacga
    20221 tcgtgttgaa gcagaagaag cgcgtctgtc cgatctgatc accggctttg ttgacccgaa
    20281 cgcagaagaa gatctggcac ctaccgccac tcacgtcggt tctgagcttt cccaggaaga
    20341 tctggacgat gacgaagatg aagacgaaga agatggcgat gacgacagcg ccgatgatga
    20401 caacagcatc gacccggaac tggctcgcga aaaatttgcg gaactgcgcg ctcagtacgt
    20461 tgtaacgcgt gacaccatca aagcgaaagg tcgcagtcac gctgccgctc aggaagagat
    20521 cctgaaactg tctgaagtat tcaaacagtt ccgcctggtg ccgaagcagt ttgactacct
    20581 ggtcaacagc atgcgcgtca tgatggaccg cgttcgtacg caagaacgtc tgatcatgaa
    20641 gctctgcgtt gagcagtgca aaatgccgaa gaaaaacttc attaccctgt ttaccggcaa
    20701 cgaaaccagc gatacctggt tcaacgcggc aattgcaatg aacaagccgt ggtcggaaaa
    20761 actgcacgat gtctctgaag aagtgcatcg cgccctgcag aaactgcagc agattgaaga
    20821 agaaaccggc ctgaccatcg agcaggttaa agatatcaac cgtcgtatgt ccatcggtga
    20881 agcgaaagcc cgccgtgcga agaaagagat ggttgaagcg aacttacgtc tggttatttc
    20941 tatcgccaag aaatacacca accgtggctt gcagttcctt gacctgattc aggaaggcaa
    21001 catcggtctg atgaaagcgg ttgataaatt cgaataccgc cgtggttaca agttctccac
    21061 ctacgcaacc tggtggatcc gtcaggcgat cacccgctct atcgcggatc aggcgcgcac
    21121 catccgtatt ccggtgcata tgattgagac tatcaacaaa ctcaaccgta tttctcgcca
    21181 gatgctgcaa gagatgggcc gtgaaccaac gccggaagaa ctggctgaac gtatgctgat
    21241 gccggaagac aagatccgca aagtgctgaa gatcgccaaa gagccaatct ccatggaaac
    21301 gccgatcggt gatgatgaag attcgcatct gggggatttc atcgaggata ccaccctcga
    21361 gctgccgctg gattctgcga ccaccgaaag cctgcgtgcg gcaacgcacg acgtgctggc
    21421 tggcctgacc gcgcgtgaag cgaaagttct gcgtatgcgt ttcggtatcg atatgaacac
    21481 cgaccacacg ctggaagaag tgggtaaaca gttcgacgtt acccgcgaac gtatccgtca
    21541 gatcgaagcg aaggcgctgc gcaaactgcg tcacccgagc cgttctgaag tgctgcgtag
    21601 cttcctggac gattaatcgg taggccggat caggtgttac gccgcacccg gcactagtcc
    21661 ctccgcacaa acgccacccg aaggtggcat aagggggtag catgtaccgc actacccctt
    21721 gatatatcaa atagttacaa gttcaaagtg gttcgaaatc gtgccattta gtccccgttt
    21781 atgggcaaaa tctggacaac tccgcaaccg gattgaacct taccgcgtcc tggagataat
    21841 caggtgcaaa gtgcgcatag gtcattgttt gctgaattgt gctgtgccct aaaatacgct
    21901 gaagggtaac aatgttccct ccatttatca tgaaatgagt tgcgaatgtg tgccgtaaaa
    21961 catgcacaga ctgaccgact ggtaaatcag gccgcacagt ccttattgca cttctgacta
    22021 tcgcataact ggggttcatt aatcttccag tcgctttgac tttaatcaga gattcaagat
    22081 ctttagctat aggaataacc cgccgcgatc cattctttgt ctccatgaaa ataaccttcc
    22141 caccaattat gtgttcacct ttaatattgg caacttcacc ccagcgcccg cctgtagcca
    22201 ggcttaacaa tacagcctta agattatcgc cagataaaag tgcggttagc ctgggaatat
    22261 catcatgtgc aagataagcc atttccggct gattaattgt tagcttgcgt atctcgtgaa
    22321 acgggttatc acccacaaac tcttcaacat caatcagctt ttcaaagaaa ccgctcatca
    22381 ttgcacaata acgatttacc gttgactgct taattccgcg attcattaat tcaaggcgat
    22441 atctgataat gttttttcgg gttaagtggt cacttctgtt aatgccaagc tcttgcatat
    22501 cacgaagaac cgcttcaatt ctgacccgat tcatcgcgcc ataacgatga ttcttaccgt
    22561 ggtacaccca ccacagctcc agaagatcgc ttaacgcttt acgttcaata ggtttttcga
    22621 cccagtcctt atcgtgataa tgcgtcagaa catggcgttc atacatcgcc gcttcaccac
    22681 gggtaggaaa tcgccgccga aatcttcttc cttcagatcc agcaggccgc acgtccacac
    22741 agtatttgcc atcagcgagc ttcttaatcg acataaggaa gccccccaat gaaaacatca
    22801 tcttgatcac aattttgaga aataaaggcc agatggaccg ttaaccagtc ttcttgccgg
    22861 agtgggatga ttccgcttcg ctttgcccaa tgtgtgcgag agccggtacc acctgcccgg
    22921 cctttggttc gacctcatcg aacaaaaacc agtcacggta tttagaaaat ctaggatgct
    22981 tgaaaagctt gatggtgatc tcaaggccag gaaggttacg accatactca taactggtca
    23041 gagcagaata agaaattcca atcatatcag cgaattcccg ctgagtgaga tcttcagatt
    23101 tacgcatgac gcgaattttt tgaggaatat ccatttgaca aactcctaca tatgtgtaga
    23161 ttaacaccac atatgaacag caaacaaaat gttctaagtg gttccaaata gtgccaatta
    23221 gaaaaagcac caatggcgga ggatatcaga tgcaagaaag cactcaaaac gcgccggagt
    23281 cactttgtgg cacacgatgc gatcaccagt cagagatatc ggaagcagaa cacctgaaga
    23341 ccggccgcaa agcccctgaa cgccaggaaa tccggctttc acaaaaccct tctcatctcc
    23401 tgtcaaaaga agggtttgcg atgtatatcg gcaagaccgt tgacgccatc gtgagcatgg
    23461 caaaagcagg gaaattaccc gctgtgtaca tggcggaccc tctcaagcct ggcggaaacg
    23521 ccgaactcta catcaaccgg aaagcgtggg atgaagcctg cgatcgactc actgaaaacg
    23581 caccgcaaga atggcacgaa tgggaaaacc gacttttttt atttaagcca gcagctggtt
    23641 cacggaataa acgcatcagt aaaaaagctg cgtgatatag aactggcaat cattggaggg
    23701 gcaagatgat taaacacacg gaaatcagac aaagaataaa aataagcgac gacgcttcag
    23761 taataatctg taatcagaac ttagatgcca ttgttcgggc tggcaacgat ttgtatttcc
    23821 attacccgac ccattgcatc gttgtgacat ttgaaacaga agagtcctta aaaaatgcaa
    23881 tggagcagac aggatttcag aatgaaacag taataagaaa agacaactgg gcataaatgc
    23941 caatacaccg ttatttcaat tcttttaact taagcgggtt tttccgatat gacacagcga
    24001 cgcaacttcc gcactggcac agaacgccac gcagcccgtt tctcaaccag tgcatcacgc
    24061 agcaacacgc actacagcct gcaggaaacg cacgccacac ctgacgggca tccggttaaa
    24121 caaaccggta aacacacctg gctgattaat gatgccggaa ttgtcattca ccggggcgca
    24181 cgcaacccga ttaccgggaa tacctgttac tccctgactc gcggagatga gcagttcggg
    24241 caggatttca ccctgcacga agcattaaga acggcagacc gattaatcag cggtcgcagt
    24301 tttcagcatc catgggggaa ttaatacatg gcaacaacga caaaagacat ggcagagata
    24361 gcatcagcct acacaattat catacaccgg ctcatagcta ataatgcccg taatgcacta
    24421 aatacagcaa aacctttatg ggaaacaaaa aaagatatca tcagcgcatt acaggggcta
    24481 cagaaatgcg ccgacctcgc aggcgattac gccgcataca tggctattga agacacgata
    24541 gagaagattg aatcaggtaa atcattaagc gattttgttt aattgttttc cggggaaata
    24601 caatggacaa agaatataag gttctcatca ataaagccct tgagcgcttt aactttcgcc
    24661 tgagtatgtc aggagttgcc gcagaacatg cagcaagaga ctcactgacc agagcaattc
    24721 gcagcctgta tgatgcagct ttttacgctg acgatctgga tgctctcaat gagctgtctg
    24781 agcttgttcg tgatgcagaa tgcggagtac atattgagcc atacgcactt gggaa
//
LOCUS       DAZFLD010000058        24476 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.058, whole genome shotgun sequence.
ACCESSION   DAZFLD010000058 DAZFLD010000000
VERSION     DAZFLD010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24476
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.058"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            93..1385
                     /locus_tag="ACHKBL_001962"
     CDS             93..1385
                     /locus_tag="ACHKBL_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000597433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFO5818841.1"
                     /translation="MIKSKIVLLSALVSCALISGCKEENKNNVSIEFMHSSVEQERQA
                     VISKLIARFEKENPGITVKQVPVEEDAYNTKVITLSRSGSLPEVIETSHDYAKVMDKE
                     QLIDRKAVATVISNVGEGAFYDGVLRIVRTEDGSAWTGVPVSAWIGGIWYRKDVLAKA
                     GLEEPKNWQQLLDVAQKLNDPANKKYGIALPTAESVLTEQSFSQFALSNQANVFNAEG
                     KITLDTPEMMQALTYYRNLAANTMPGSNDIMEVKDAFMNGTAPMAIYSTYILPAVIKE
                     GDPKNVGFVVPTEKNSAVYGMLTSLTITVGQKAEETEAAEKFVTFMEQADNIADWVMM
                     SPGAALPVNKAVVTTATWKDNDVIKALGELPNQLISELPNIQVFGAVGDKNFTRMGDV
                     TGSGVVSSMVHNVTVGKADLPGTLQTSQKKLDELVEQR"
     gene            1406..2287
                     /locus_tag="ACHKBL_001963"
     CDS             1406..2287
                     /locus_tag="ACHKBL_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFO5818842.1"
                     /translation="MNRLFSGRSDMPFALLLLAPSLLLLGGLVAWPMVSNIEISFLRL
                     PLNPNIESTFVGVSNYVRILSDPGFWHSLWMTVWYTALVVAGSTVLGLAVAMFFNREF
                     RLRKTARSLVILSYVTPSISLVFAWKYMFNNGYGIVNYLGVDLLHLYEQAPLWFDNPG
                     SSFVLVVLFAIWRYFPYAFISFLAILQTIDKSLYEAAEMDGANAWQRFRIVTLPAIMP
                     VLATVVTLRTIWMFYMFADVYLLTTKVDILGVYLYKTAFAFNDLGKAAAISVVLFIII
                     FAVILLTRKRVNLNGNK"
     gene            2274..3116
                     /locus_tag="ACHKBL_001964"
     CDS             2274..3116
                     /locus_tag="ACHKBL_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFO5818843.1"
                     /translation="MATNKRTLSRIGFYCGLALFLIITLFPFFVMLMTSFKSAKEAIS
                     LHPTLLPQQWTLEHYVDIFNPMIFPFVDYFRNSMVVSVVSSVVAVFLGILGAYALSRL
                     RFKGRMTINASFYTVYMFSGILLVVPLFKIITALGIYDTEMALIITMVTQTLPTAVFM
                     LKSYFDTIPDEIEEAAMMDGLNRLQIIFRITVPLAMSGLISVFVYCFMVAWNDYLFAS
                     IFLSSASNFTLPVGLNALFSTPDYIWGRMMAASLVTALPVVIMYALSERFIKSGLTAG
                     GVKG"
     gene            3147..4199
                     /locus_tag="ACHKBL_001965"
     CDS             3147..4199
                     /locus_tag="ACHKBL_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HFO5818844.1"
                     /translation="MKKLVATAPRVAALVEYEDRAILANEVKIRVRFGAPKHGTEVVD
                     FRAASPFIDEDFNGEWQMFTPRPADAPRGIEFGKFQLGNMVVGDIIECGSDVTDYAVG
                     DSVCGYGPLSETVIINAVNNYKLRKMPQGSSWKNAVCYDPAQFAMSGVRDANVRVGDF
                     VVVVGLGAIGQIAIQLAKRAGASVVIGVDPIAHRCEIARRHGADFCLNPIGSDVGKEI
                     KTLTGKQGADVIIETSGYADALQSALRGLAYGGTISYVAFAKPFAEGFNLGREAHFNN
                     AKIVFSRACSEPNPDYPRWSRKRIEETCWELLMNGYLNCEDLIDPVVTFANSPESYMQ
                     YVDQHPEQSIKMGVTF"
     gene            4218..5006
                     /locus_tag="ACHKBL_001966"
                     /pseudo
     CDS             4218..5006
                     /locus_tag="ACHKBL_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415830.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
     gene            5016..6071
                     /locus_tag="ACHKBL_001967"
     CDS             5016..6071
                     /locus_tag="ACHKBL_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFO5818845.1"
                     /translation="MKSAMTSSPLRVAIIGAGQVADKVHASYYCTRNDLELVAVCDSR
                     LSQAQALAEKYGNASVWDDPQAMLLAVKPDVVSVCSPNRFHYEHTLMALEAGCHVMCE
                     KPPAMTAEQAREMCDTARKQGKVLAYDFHHRFALDTQQLREQVTNGVLGEIYVTTARA
                     LRRCGVPGWGVFTNKELQGGGPLIDIGIHMLDAAMYVLGFPAVKSVTAHSFQKIGTQK
                     SCGQFGEWDPATYSVEDSLFGTIEFHNGGILWLETSFALNIREQSIMNVSFCGDKAGA
                     TLFPAHIYTDNNGELMTLMQREMADDNRHLRSMEAFINHVQGKPVMIADAEQGYIIQQ
                     LVAALYQSAETGTRVEL"
     gene            6068..8335
                     /gene="ycjT"
                     /locus_tag="ACHKBL_001968"
     CDS             6068..8335
                     /gene="ycjT"
                     /locus_tag="ACHKBL_001968"
                     /EC_number="2.4.1.230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kojibiose phosphorylase"
                     /protein_id="HFO5818846.1"
                     /translation="MIRPVTLTEPHFSQHTLNKYASLMAQGNGYLGLRASHEEDYTRQ
                     TRGMYLAGLYHRAGKGEINELVNLPDVVGMEIAINGEIFSLSREAWQRELNFASGELR
                     RNVVWCTSNGAGYTITSRRFVSADQLPLIALEITITPLDADALVLISTGIDATQTNHG
                     RQHLDETQVRVFGQHLMQGIYTTQDGRSDVAISCCCKVSGDVQQCYTAKERRLLQHTC
                     AQLHAGETLTLQKRVWIDWRDDRQAALDEWGSASLRQLEMCAQQSYDQLLAASTENWR
                     QWWQKRRITVNGGDAHDQQALDYALYHLRIMTPAHDERSSIAAKGLTGEGYKGHVFWD
                     TEVFLLPFHLFSDPTVARSLLRYRWHNLPGAQEKARRNGWQGALFPWESARSGEEETP
                     EFAAINIRTGLRQKVASAQAEHHLVADIAWAVIQYWQATGDESFIAHEGMALLLGTAK
                     FWISRAVQVNDRLEIHNVIGPDEYTEHVNNNAFTSYMAYYNVQQALNIARKFGCSDDA
                     FIHRAEIFLKELRLPEIQPDGVLPQDDSFMAKPAINLAKYKAAAGKQTILLDYSRAEV
                     NEMQILKQADVVMLNYMLPEQYSAASCLANLQFYEPRTIHDSSLSKAIHGIVAARCGL
                     LAQSYQFWREGTEIDLGADPHSCDDGIHAAATGAIWLGAIQGFAGVSVRNGELHLNPA
                     LPEQWQQLSFPLFWQGCELQVTLDAQRIAIRTSAPVSLRLNGQLISVAEESVFCLGDF
                     ILPFNGTATTHQEDE"
     gene            8332..8991
                     /gene="pgmB"
                     /locus_tag="ACHKBL_001969"
     CDS             8332..8991
                     /gene="pgmB"
                     /locus_tag="ACHKBL_001969"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFO5818847.1"
                     /translation="MKLQGVIFDLDGVITDTAHLHFKAWQQIAAEIGISIDAQFNESL
                     KGISRDESLRRILQHGGKEGDFNPQERAQLAYRKNLLYVHSLRELTVNAVLPGIRNLL
                     ADLRAQQIPIGLASVSLNAPTILAALELREFFTFCADASQLKNSKPDPEIFLAACAGL
                     GVPPQACIGIEDAQAGIDAINASGMRSVGIGAGLTGAQLLLPSTDSLTWPRLSAFWQN
                     V"
     gene            9005..10087
                     /locus_tag="ACHKBL_001970"
     CDS             9005..10087
                     /locus_tag="ACHKBL_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO5818848.1"
                     /translation="MAQLSLQHIQKIYDNQVHVVKDFNLEIADKEFIVFVGPSGCGKS
                     TTLRMIAGLEEISGGDLLIDGKRMNDVPAKERNIAMVFLNYALYPHMTVYDNMAFGLK
                     MQKIANEVIDERVNWAAQILGLREYLKRKPGALSGGQRQRVALGRAIVREAGVFLMDE
                     PLSNLDAKLRVQMRAEISKLHQKLNTTMIYVTHDQTEAMTMATRIVIMKDGIVQQVGA
                     PKTVYNQPANMFVAGFIGSPAMNFIRGTIDGDKFVTETLKLTISEEKLAVLKTQESLH
                     KPIVMGIRPEDIHPDAQEENNISAKISVAELTGAEFMLYTTVGGHELVVRAGALNDYH
                     AGENITIHFDMTKCHFFDAETEIAIR"
     gene            10132..11037
                     /gene="ompG"
                     /locus_tag="ACHKBL_001971"
     CDS             10132..11037
                     /gene="ompG"
                     /locus_tag="ACHKBL_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monomeric porin OmpG"
                     /protein_id="HFO5818849.1"
                     /translation="MKKLLPCTALVMCAGMACAQAEEKNDWHFNIGAMYEIENVEGYG
                     EDMDGLAEPSVYFNAANGPWRISLAYYQEGPVDYSAGKRGTWFDRPELEVHYQFLESD
                     DFSFGLTGGFRNYGYHYVDEPGKDTANMQRWKIAPDWDVKLTDDLRFNGWLSMYKFAN
                     DLNTTGYADTRVETETGLQYTFNETVALRVNYYLERGFNMDDSRNNGEFSTQEIRAYL
                     PLTLGNHSVTPYTRIGLDRWSNWDWQDDIEREGHDFNRVGLFYGYDFQNGLSVSLEYA
                     FEWQDHDEGDSDKFHYAGVGVNYSF"
     gene            complement(11088..12086)
                     /locus_tag="ACHKBL_001972"
     CDS             complement(11088..12086)
                     /locus_tag="ACHKBL_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFO5818850.1"
                     /translation="MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEEL
                     QYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEEGFDVILQT
                     SHNLAEDLQKCESKIKQKMIKGIIMLSSPADESFFAQLDKYDIPVVVIGKVEGQYAHV
                     YSVDTDNFGDSIALTDALIESGNQNIACLHAPLDVHVSVDRVNGYKQSLAAHNIAVRD
                     EWIVDGGYTHETALQAARQLLSQSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAV
                     VGYSNETLSFILTPAPGGIDVPTQELGQQSCELLFRLIAGKPSPQNITVATHMSLK"
     gene            12241..13638
                     /locus_tag="ACHKBL_001973"
     CDS             12241..13638
                     /locus_tag="ACHKBL_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HFO5818851.1"
                     /translation="MKRLKNELNALVNRGVDRHLRLAVTGLSRSGKTAFITAMVNQLL
                     NIHAGARLPLLSAVREERLLGVKRIPQRDFGIPRFTYDEGLAQLYGDPPAWPTPTRGV
                     SEIRLALRYKSNDSLLRHFKDTSTLYLEIVDYPGEWLLDLPMLAQDYLSWSRQMTGLL
                     NGQRGEWSAKWRMMCEGLDPLAPADENRLADIAAAWTDYLHHCKQQGLHFIQPGRFVL
                     PGDMAGAPALQFFPWPDVDAWGESKLAQADKHTNAGMLRERFNYYCEKVVKGFYKNHF
                     LRFDRQIVLVDCLQPLNSGPQAFNDMRLALTQLMQSFHYGQRTLFRRLFSPVIDKLLF
                     AATKADHVTIDQHANMVSLLQQLIQDAWQNAAFEGISMDCLGLASVQATTSGIIDVNG
                     EKIPALRGNRLSDGAPLTVYPGEVPARLPGQAFWDKQGFQFEAFRPQVMDVDKPLPHI
                     RLDAALEFLIGDKLR"
     gene            13635..14696
                     /locus_tag="ACHKBL_001974"
     CDS             13635..14696
                     /locus_tag="ACHKBL_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjF family protein"
                     /protein_id="HFO5818852.1"
                     /translation="MTEPLKPRIDFDGPLEVDQNPKFRAQQTFDENQAQNFAPATLDE
                     AQEEEGQVEAVMDAALRPKRSLWRKMVMGGLALFGASVVGQGVQWTMNAWQTQDWVAL
                     GGCAAGALIIGAGVGSVVTEWRRLWRLRQRAHERDEARDLLHSHGTGKGRAFCEKLAQ
                     QAGIDQSHPALQRWYASIHETQNDREVVSLYAHLVQPVLDAQARREISRSAAESTLMI
                     AVSPLALVDMAFIAWRNLRLINRIATLYGIELGYYSRLRLFKLVLLNIAFAGASELVR
                     EVGMDWMSQDLAARLSTRAAQGIGAGLLTARLGIKAMELCRPLPWIEDDKPRLGDFRR
                     QLIGQVKETLQKGKTPSEK"
     gene            14844..16385
                     /gene="tyrR"
                     /locus_tag="ACHKBL_001975"
     CDS             14844..16385
                     /gene="tyrR"
                     /locus_tag="ACHKBL_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TyrR"
                     /protein_id="HFO5818853.1"
                     /translation="MRLEVFCEDRLGLTRELLDLLVLRGIDLRGIEIDPIGRIYLNFA
                     ELEFESFSSLMAEIRRIAGVTDVRTVPWMPSEREHLALSALLEALPEPVLSVDMKSKV
                     DMANPASCQLFGQKLDRLRNHTAAQLINGFNFLRWLESEPQDSHNEHVVINGQNFLME
                     ITPVYLQDENDQHVLTGAVVMLRSTIRMGRQLQNVAAQDVSAFSQIVAVSPKMKHVVE
                     QAQKLAMLSAPLLITGDTGTGKDLFAYACHQASPRAGKPYLALNCASIPEDAVESELF
                     GHAPEGKKGFFEQANGGSVLLDEIGEMSPRMQAKLLRFLNDGTFRRVGEDHEVHVDVR
                     VICATQKNLVELVQKGVFREDLYYRLNVLTLNLPPLRDCPQDIMPLTELFVARFADEQ
                     GVPRPKLAADLNTVLTRYAWPGNVRQLKNAIYRALTQLDGYELRPQDILLPDYDAATV
                     AVGEDAMEGSLDEITSRFERSVLTQLYRNYPSTRKLAKRLGVSHTAIANKLREYGLSQ
                     KKNEE"
     gene            complement(16429..16935)
                     /gene="tpx"
                     /locus_tag="ACHKBL_001976"
     CDS             complement(16429..16935)
                     /gene="tpx"
                     /locus_tag="ACHKBL_001976"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HFO5818854.1"
                     /translation="MSQTVHFQGNPVTVANSIPQAGSKAQTFTLVAKDLSDVTLGQFA
                     GKRKVLNIFPSIDTGVCAASVRKFNQLATEIDNTVVLCISADLPFAQSRFCGAEGLNN
                     VITLSTFRNAEFLQAYGVAIADGPLKGLAARAVVVIDENDNVIFSQLVDEITTEPDYE
                     AALAVLKA"
     gene            17054..18019
                     /gene="ycjG"
                     /locus_tag="ACHKBL_001977"
     CDS             17054..18019
                     /gene="ycjG"
                     /locus_tag="ACHKBL_001977"
                     /EC_number="5.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016855 - racemase and epimerase activity,
                     acting on amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-Ala-D/L-Glu epimerase"
                     /protein_id="HFO5818855.1"
                     /translation="MRTVKVFEEGWPLHTPFVIARGSRSEARVVVVELEEEGIKGTGE
                     CTPYPRYGESDASVMAQIMSVVPQLEKGLTREELQKILPAGAARNALDCALWDLAARK
                     QQESLADLIGITLPGTVITAQTVVIGTPDQMANSASTLWQAGAKLLKVKLDNHLISER
                     MVAIRTAVPDATLIVDANESWRAEGLAARCQLLADLGVAMLEQPLPAQDDAALENFIH
                     PLPICADESCHTRSNLKALKGRYEMVNIKLDKTGGLTEALALATEARAQGFSLMLGCM
                     LCTSRAISAALPLVPQVSFADLDGPTWLAVDVEPALQFTTGELHL"
     gene            complement(17994..18722)
                     /gene="mpaA"
                     /locus_tag="ACHKBL_001978"
     CDS             complement(17994..18722)
                     /gene="mpaA"
                     /locus_tag="ACHKBL_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide amidase MpaA"
                     /protein_id="HFO5818856.1"
                     /translation="MTVTRPRAERGAFPPGTEHYGRSLLGAPLIWFPAPAASHESGLI
                     LAGTHGDENSSIVTLSCALRTLTPSLRRHHVVLCVNPDGCQLGLRANANGVDLNRNFP
                     AANWKEGETVYRWNSAAEERDVVLLTGDKPGSEPETQALCQLIHRIQPAWVVSFHDPL
                     ACIEDPRHSELGEWLAQAFELPLVTSVGYETPGSFGSWCADLNLHCITAEFPPISSDE
                     ASEKYLFAMANLLRWHPKDAIRPL"
     gene            complement(19013..19651)
                     /locus_tag="ACHKBL_001979"
     CDS             complement(19013..19651)
                     /locus_tag="ACHKBL_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFO5818857.1"
                     /translation="MKNVLILGAGGQIARHVINQLADKQTIKQTLFARQPAKIHKPYP
                     TNSKIIMGDVLNHAALKQAMQGQDVVYANLTGEDLDIQANSVIAAMKAGDVKRLIFVL
                     SLGIYDEVPGKFGEWNNAVIGEPLKPFRRAADAIEASGLEYTILRPAWLTDEDIIDYE
                     LTSRNEPFKGTIVSRKSVAALITDIIDKPEKHIGENIGINQPGTDGDKPFFM"
     gene            complement(19722..20642)
                     /locus_tag="ACHKBL_001980"
     CDS             complement(19722..20642)
                     /locus_tag="ACHKBL_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFO5818858.1"
                     /translation="MMNNKVSFTNSNNPTISLSAVIYFPPKFDETRQYPAIVVSHPGG
                     GVKEQTAGTYAEKLAEKGFVTIAYDASYQGESGGEPRQLENPYIRTEDVSAVIDYLTT
                     LSYVDNSRIGAMGICAGAGYTANAAIQDRRIKAIGTVSAVNIGSMFRNGWENNVKSID
                     ALPYVDAGSNARTSDISSREYATMPLAPMKESDAPNEELRQAWEYYHTPRAQYPTAPG
                     YATLRSLNQIITYDAYHMAEVYLTQPIQIVAGSQAGSKWMSDDLYDRASSQDKRYHIV
                     EGANHMDLYDGKVYVAEAISVLAPFFEETL"
     gene            20780..21679
                     /gene="pgrR"
                     /locus_tag="ACHKBL_001981"
     CDS             20780..21679
                     /gene="pgrR"
                     /locus_tag="ACHKBL_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator PgrR"
                     /protein_id="HFO5818859.1"
                     /translation="MKREEIADLMAFVVVAEERSFTRAAARLSMAQSALSQIVRRIEE
                     RLGLRLLTRTTRSVVPTEAGEHLLSVLGPMLHDIDSALTSLSDLQNRPSGTIRITTVE
                     HAAKTILLPAMRTFLKSHPEIDIQLTIDYGLTDVVSERFDAGVRLGGEMDKDMIAIRI
                     GPDIPMAIVGSPDYFSRRSAPTSVSQLIDHQAINLYLPTSGTANRWRLIRGGREVRVR
                     MEGQLLLNTIDLIIDAAIDGHGLAYLPYDQVERAIKEKKLIRVLDKFTPDLPGYHLYY
                     PHRRHAGSAFSLFIDRLKYKGAV"
     gene            22016..23629
                     /gene="mppA"
                     /locus_tag="ACHKBL_001982"
     CDS             22016..23629
                     /gene="mppA"
                     /locus_tag="ACHKBL_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide ABC transporter
                     substrate-binding protein MppA"
                     /protein_id="HFO5818860.1"
                     /translation="MKHSVSVTCCALLVSSISLSYAAEVPSGTVLAEKQELVRHIKDE
                     PASLDPAKAVGLPEIQVIRDLFEGLVNQNEKGEIVPGVATQWKSNDNRIWTFTLRDNA
                     KWADGTPVTAQDFVYSWQRLVDPKTLSPFAWFAALAGINNAQAIIDGKAAPDQLGVTA
                     VDAQTLKIQLDKPLPWFVNLTANFAFFPVQKANVESSKEWTKPGNLIGNGAYVLKERV
                     VNEKLVVVPNTHYWDNAKTVLQKVTFLPINQESAATKRYLAGDIDITESFPKNMYQKL
                     LKDIPGQVYTPPQLGTYYYAFNTQKGPTADQRVRLALSMTIDRRLMTEKVLGTGEKPA
                     WHFTPDVTAGFTPEPSPFEQMSQEELNAQAKTLLSAAGYGPQKPLKLTLLYNTSENHQ
                     KIAIAVASMWKKNLGVDVKLQNQEWKTYIDSRNTGNFDVIRASWVGDYNEPSTFLTLL
                     TSTHSGNISRFNNPVYDKVLAQASTENTVKARNADYNTAEKILMEQAPIAPIYQYTNG
                     RLIKPWLKGYPINNPEDVAYSRTMYIVKH"
     gene            complement(23680..>24476)
                     /gene="ynaI"
                     /locus_tag="ACHKBL_001983"
     CDS             complement(23680..>24476)
                     /gene="ynaI"
                     /locus_tag="ACHKBL_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="low conductance mechanosensitive channel YnaI"
                     /protein_id="HFO5818861.1"
                     /translation="FICTSLIAVILTIKLFLLINQFEKQQIKKGRDITSARIMSRIIK
                     ITIIVVLVLLYGEHFGMSLSGLLTFGGIGGLAVGMAGKDILSNFFSGIMLYFDRPFSI
                     GDWIRSPDRNIEGTVAEIGWRITKITTFDNRPLYVPNSLFSSISVENPGRMTNRRITT
                     TIGLRYEDAAKVGVIVEAVREMLKNHPAIDQRQTLLVYFNQFADSSLNIMVYCFTKTT
                     VWAEWLAAQQDVYLKIIDIVQSHGADFAFPSQTLYMDNITPPDQGR"
BASE COUNT         6004 a         5867 c         6543 g         6062 t
ORIGIN      
        1 tcgggatctg attagtgaag ttgatatatt gggtaatgaa ataacactgc atccctggca
       61 ggttatgtgg attaaataaa aaggaacatt tcatgattaa atcaaaaatc gtgctgttat
      121 cagcactggt ttcctgcgcc ctgatttcag gctgtaaaga agaaaataaa aataatgtat
      181 ccatcgaatt tatgcattct tcggtggagc aggagcgcca ggccgtcatc agcaaattga
      241 ttgcccgttt tgaaaaagaa aacccaggta tcaccgtaaa acaagtgccc gtggaagaag
      301 acgcctataa caccaaagtc attacccttt cacgtagcgg ttcgctgccg gaagtgatcg
      361 aaaccagcca tgactacgcc aaagtgatgg acaaagagca gcttatcgat cgtaaagcgg
      421 ttgccacggt catcagcaac gtcggtgaag gcgcgttcta cgatggcgta ctgcggattg
      481 tgcgcaccga agatggcagc gcctggaccg gtgttcctgt cagcgcatgg attggcggga
      541 tctggtatcg caaagatgtg ctggcaaaag ctgggctgga ggagccaaaa aactggcagc
      601 aattgctgga cgttgcacag aaactgaatg acccggcgaa taaaaaatac ggtattgcgc
      661 tgcctacagc agaaagcgtg ttgacggaac agtctttctc ccagtttgcg ttatccaatc
      721 aggctaacgt ctttaacgcc gaaggcaaaa tcacccttga tacaccagag atgatgcagg
      781 cgctgaccta ttaccgcaac cttgctgcca acaccatgcc aggttctaac gacatcatgg
      841 aagtgaaaga cgcttttatg aacggcaccg cgccgatggc gatttactcc acctatatcc
      901 ttccggctgt gattaaagaa ggcgatccga aaaacgttgg tttcgttgta cccaccgaga
      961 aaaactctgc ggtctacggc atgttgacct cgctgaccat taccgtcggg caaaaggccg
     1021 aagagacgga agcggctgaa aaatttgtca cctttatgga gcaggcagac aacattgccg
     1081 actgggtgat gatgtcgcca ggcgcggcgc tgccggtgaa caaagcggtt gtcactaccg
     1141 ccacctggaa agacaacgac gttattaagg cgctgggtga gctgccaaat cagttaatca
     1201 gcgaactgcc aaatattcag gtctttggcg cagtagggga taaaaatttt acccgcatgg
     1261 gcgatgtgac gggttctggc gtggtgagct ccatggtgca taacgtcacc gtgggtaaag
     1321 ccgacctccc tggcacgctg caaacgagcc agaaaaagct ggatgagttg gttgaacagc
     1381 gctaaaccca ggacaggaat ccgcaatgaa caggcttttt tcaggtcgtt ccgatatgcc
     1441 ctttgcgctg ttgcttctcg cgcccagctt attactgctg ggcggtctgg tggcgtggcc
     1501 gatggtgtcg aatatcgaaa tcagtttttt acgtctgccg cttaatccca acatcgagtc
     1561 aacgtttgtt ggggtgagca actacgtgcg tattctctcc gatcccggct tctggcactc
     1621 gctgtggatg acggtctggt ataccgcgct ggtggtggcg ggcagcaccg tgctcgggct
     1681 ggccgtggcg atgtttttca accgcgagtt ccgcctgcgc aaaaccgcgc gttcgctggt
     1741 gatcctctcc tacgtaacgc cgtctatttc gctggtgttc gcctggaaat acatgttcaa
     1801 caacggttac ggcattgtga actacctcgg cgtcgatctt ctgcatctct atgagcaggc
     1861 accgctgtgg ttcgataatc cgggcagtag ctttgtgctg gtggtgctgt tcgccatctg
     1921 gcgctacttc ccctatgcct ttatttcgtt tctggcgatt ttgcagacca tcgacaaatc
     1981 gctgtacgaa gcggcagaga tggatggcgc taatgcctgg caacggtttc gtatcgtcac
     2041 gctgcccgcg attatgccgg tcctggcgac ggtggtgacc ctgcgcacca tctggatgtt
     2101 ctacatgttc gcggatgttt atctgctgac gaccaaggtc gatattctcg gtgtctatct
     2161 ctacaaaacc gcctttgcct ttaatgactt agggaaagcg gcggcgatct cggtggtgct
     2221 cttcatcatt attttcgctg tcattctgct gaccagaaaa cgggtgaacc tcaatggcaa
     2281 caaataaacg cacactcagt cgcatcggtt tttactgcgg gctggcgctg tttctcatca
     2341 tcacgctgtt tccgtttttt gtgatgctga tgacctcgtt caagagcgca aaagaggcga
     2401 tctcgctgca tcctacgctg ctgccgcaac agtggacgct ggagcattat gtcgacattt
     2461 ttaacccgat gattttcccg tttgtcgact acttccgtaa cagcatggtg gtgtcggtgg
     2521 tttcatccgt ggtggcggta tttctcggca ttctcggggc ttacgcgctt tcccgcctgc
     2581 gctttaaagg gcggatgacc atcaacgcca gcttttacac ggtgtatatg ttctccggca
     2641 ttttgctggt ggtgccgctg ttcaaaatca tcaccgcgct tggcatatac gacaccgaga
     2701 tggcgctgat cattaccatg gtaacgcaaa ccctgcccac cgccgtgttt atgctgaaaa
     2761 gctacttcga caccatccct gatgagatcg aagaagcggc gatgatggat ggcctcaacc
     2821 gtctgcaaat catcttccgt atcaccgtgc ccctggcgat gtccgggctg atatccgtat
     2881 tcgtctactg ctttatggtg gcatggaacg actatctgtt tgcgtcgatt ttcctctcca
     2941 gcgccagcaa tttcacctta ccagtcggcc tgaacgcgct gttcagtacg cctgattaca
     3001 tctggggacg gatgatggcg gcctcgctgg tgaccgcatt gccggtggtg attatgtatg
     3061 cgctttcgga gcgttttatt aagagtggtt tgaccgccgg tggcgtgaag ggctaaagcg
     3121 gccagtttct ttacaaggag ttttacatga aaaagttagt agccacagca ccgcgcgtag
     3181 ctgcgctggt tgagtatgaa gatcgggcga ttttagccaa tgaagtgaag atccgcgtgc
     3241 gcttcggcgc accgaagcac gggacggaag tggtcgattt ccgcgctgcc agtccgttta
     3301 tcgatgaaga ctttaacggc gaatggcaga tgttcacccc gcgtccggca gatgcgccgc
     3361 gcggcattga gtttggcaaa ttccagcttg gcaacatggt ggttggcgac attatcgagt
     3421 gcggcagcga cgttaccgac tacgcggtgg gcgacagcgt atgcggctac ggcccgctct
     3481 ccgagacggt catcattaac gcagtgaata actacaagct gcgcaaaatg ccgcaaggca
     3541 gttcgtggaa aaacgctgtc tgctacgacc cggcgcagtt tgccatgagc ggcgtgcgcg
     3601 atgccaatgt gcgcgtgggc gattttgtgg tggtggtagg gcttggcgcg atcggtcaaa
     3661 ttgccattca actggctaaa cgtgctggcg cgtcggtggt gattggcgtc gatcctatcg
     3721 cccatcgctg tgagattgcc cgtcgccatg gcgcggattt ctgccttaac cccattggca
     3781 gtgacgtagg caaagagatc aaaacgctaa ccggcaagca gggtgccgat gtgattatcg
     3841 aaaccagcgg ctatgccgac gcgctgcaat cggcgctacg aggtctggca tatggcggca
     3901 ccatctcgta tgtcgcgttt gccaaaccgt ttgccgaagg ttttaacctc ggacgcgaag
     3961 cccatttcaa taacgccaaa attgttttct cccgtgcgtg cagcgagccg aacccggatt
     4021 atccgcgctg gagccgcaaa cgtatcgaag aaacctgttg ggaactgctg atgaacggtt
     4081 atctcaattg tgaagattta atcgacccgg tggtgacctt tgccaatagc ccggaaagtt
     4141 acatgcagta tgtcgaccag catccggaac agagcatcaa aatgggcgtt acgttttaat
     4201 tcaaaggaat gaaaaaaatg aaaatcggca cacagaatca ggcgttcttt ccggaaaaca
     4261 ttctggagaa atttcgttat atctaagaga tgggtttcga tggttttgag atcgacggca
     4321 aactgctggt caacaatctc gaagaagtca aagcagcaat caaagaaacc ggtttaccgg
     4381 tgatcaccgc ctgcggtggc tatgacggtt ggattggcga ctttatcgaa gagcgtcgac
     4441 ttaatggctt aaagcagatc gaacgcattc ttgaagctct ggcagaagtg ggcggtaaag
     4501 gtatcgtcgt tccggctgca tggggcatgt ttaccttccg cttaccgccg atgacctcgc
     4561 cgcgtagcct ggacggcgac cgcaaaatgg tgagtgattc cctgcgcgta ctggaaaagg
     4621 tggccgcacg taccggaacc gtggtgtacc tcgaaccgtt aaaccgttat caggatcaca
     4681 tgatcaacac tctcgccgat gcccgccgtt atatcgtcga aaacgatctt aaacatgtac
     4741 agattatcgg cgatttctat cacatgaata tcgaagaaga taacctggcg caggcgctgc
     4801 atgacaaccg cgacctgctc ggtcatgtgc atattgccga taaccatcgc taccagccgg
     4861 gcagcggcac cctggatttc cacgcgctgt tagaacagct gcgcgcggat aactttcagg
     4921 gctatgtggt gtacgaaggg cgcatccggg cggaagatcc agcccaggcg taccgtgatt
     4981 ctttggcctg gttgcgtacc tgctaagagg tctttgtgaa aagtgcaatg acaagctctc
     5041 cgctgcgggt cgcgataata ggcgcgggcc aggtggcgga taaagttcat gcttcgtact
     5101 actgcacccg caacgatctg gaactggtgg ctgtctgtga cagccgcctt tcccaggcgc
     5161 aggcgctggc agaaaaatac gggaatgcat ccgtgtggga cgatccgcag gccatgctgc
     5221 tggcggtgaa acctgatgtg gttagcgtct gctcacctaa ccgttttcat tacgaacata
     5281 ccctgatggc gctggaagcg ggctgccatg tgatgtgcga aaaaccgccc gccatgacgg
     5341 cagaacaggc gcgggaaatg tgcgataccg cgcgcaaaca gggcaaggtg ctggcctacg
     5401 actttcacca tcgttttgca ctcgatacgc aacagctgcg tgaacaggtg accaacggcg
     5461 ttttgggaga gatttacgtt accaccgccc gcgccctgcg tcgttgcggt gttcccggct
     5521 ggggtgtctt tactaataaa gaattgcagg gcggaggccc gctgatcgac atcggcattc
     5581 atatgctgga tgcggcgatg tatgtgctgg gttttccagc ggtgaaaagc gtgactgcgc
     5641 atagctttca aaagatcggc acgcaaaaga gctgcggtca atttggcgag tgggacccgg
     5701 caacctacag cgtcgaagat tcgctgtttg gcaccattga atttcataac ggcggcattc
     5761 tgtggcttga gacctcattt gcactcaaca tccgcgaaca gtcgattatg aacgtcagct
     5821 tttgtggtga taaagccggg gcgacgctgt ttccagcaca tatctacacc gataacaacg
     5881 gtgagttaat gacgctgatg caacgggaaa tggcagacga caaccgtcat ctgcgcagta
     5941 tggaagcctt tatcaaccac gtacaaggta agcccgtgat gatagccgac gccgagcagg
     6001 gatacatcat ccagcaactg gtggcggcgc tttatcaatc cgcagaaaca gggacgcgtg
     6061 tggaattatg atcaggccag taacgttaac ggaaccccat ttcagccagc ataccctgaa
     6121 caagtatgct tcgctgatgg cgcaggggaa cggctatctt gggcttcgcg ccagccatga
     6181 agaagattac acgcgccaga cgcgcggcat gtatctggca gggctttatc atcgggcggg
     6241 taaaggtgaa atcaacgaac tggtgaacct gcctgatgtc gtggggatgg agattgccat
     6301 aaatggtgag attttctcgt tatcccgcga agcatggcag cgtgagctta actttgccag
     6361 tggcgaatta cgccgcaacg ttgtctggtg taccagcaac ggcgcaggtt acaccatcac
     6421 cagccgtcgc tttgtttcgg cagaccaact gccgctcatt gcgctggaaa tcactattac
     6481 gccactggac gccgacgcgt tagtgctgat ttcaacaggc attgacgcca cgcaaaccaa
     6541 ccacggtcgc caacatctcg acgaaaccca ggtgcgggta tttggtcagc atctgatgca
     6601 ggggatctac accacccagg atgggcgcag tgatgtggcc atcagctgtt gctgtaaggt
     6661 gagcggtgat gtgcagcaat gctataccgc caaagagcgc cgtttactgc aacatacctg
     6721 cgcgcagctt cacgcaggtg agacgctgac gctgcaaaaa cgggtgtgga tcgactggcg
     6781 ggacgacaga caagccgctt tagacgaatg gggcagtgcg tcgcttcgtc agcttgaaat
     6841 gtgcgcgcag cagagttacg accaacttct tgcagcatca acagaaaact ggcgtcaatg
     6901 gtggcagaaa cgtcgtatca cggtaaacgg cggcgatgcg cacgatcagc aagcgttaga
     6961 ttatgcgctt tatcacctgc gcatcatgac gcctgcccac gatgagcgca gcagcattgc
     7021 ggcgaaaggt ttaaccggcg aaggctacaa aggccacgtt ttctgggata cagaagtatt
     7081 tttgctaccg ttccatctgt ttagcgatcc gacggttgcc cgaagtttac tgcgttatcg
     7141 ctggcacaac ttgcctggcg cgcaggagaa agcgcgacgc aacggctggc agggcgctct
     7201 gtttccgtgg gaaagcgcgc gcagcggcga agaagagacg ccggaatttg ccgccattaa
     7261 catccgcacc ggactgcgcc agaaagtggc ctcggcgcag gctgaacacc atttggtggc
     7321 tgatatcgcc tgggcggtta ttcaatactg gcaggcaacg ggggatgaaa gtttcattgc
     7381 ccacgaaggc atggcgttac ttctgggaac cgcaaaattc tggattagcc gcgctgtaca
     7441 ggtaaacgat cgtctggaaa ttcacaatgt tattgggcca gacgaatata ccgaacatgt
     7501 caataataac gccttcacca gctatatggc gtattacaac gtccagcagg cgctgaatat
     7561 cgctcgtaag tttggttgta gcgacgacgc gtttattcat cgtgccgaaa tattcctcaa
     7621 agaactgcgg ctgccagaaa ttcagcctga cggcgttttg ccgcaggatg attcgtttat
     7681 ggcgaaacca gcgattaatc tggcgaaata caaagcggcg gcggggaagc aaaccattct
     7741 gctggattat tcacgcgcag aagtgaacga gatgcagatc ctcaaacaag ctgatgtggt
     7801 gatgctcaat tacatgctgc cggagcagta ctcagcggca tcgtgtcttg ccaatctgca
     7861 attttatgaa ccgcgcacca ttcacgactc ttcactgagt aaagcgatcc acggcattgt
     7921 tgccgcacgc tgtggcctgt tggcgcaaag ttatcagttc tggcgcgagg ggactgaaat
     7981 cgatcttggt gctgatccgc atagctgtga tgacggtatc cacgcagccg caactggcgc
     8041 tatttggctg ggggcgattc agggttttgc cggggtgagc gtgcgtaacg gtgaattgca
     8101 tctcaatcca gcgttacctg agcagtggca acagttgtct ttccctctgt tctggcaggg
     8161 ctgcgaatta caggtcactc tcgacgcgca gcgtattgcg attcgaactt ctgcgcccgt
     8221 ttcactgcgt ttgaacgggc agcttatatc tgtggctgaa gaatctgttt tctgtttggg
     8281 tgattttatt ttgcccttca atgggaccgc taccacgcat caggaggatg aatgaaactg
     8341 caaggggtaa ttttcgatct ggatggtgta atcaccgata ccgcgcatct gcatttcaag
     8401 gcgtggcagc agattgccgc tgaaattggc atcagcattg atgcgcagtt taacgaatcc
     8461 ctaaaaggga tcagccgcga tgagtctctg cggcgcattc tgcaacacgg gggcaaagag
     8521 ggcgacttta acccgcagga gagggcgcaa ctggcgtatc gcaaaaatct gctctacgtc
     8581 cactcactgc gcgaactgac ggttaatgct gtgctgcccg gcattcgcaa tttgctggca
     8641 gatctccgtg cacagcagat ccctataggg ctggcttctg tctccctgaa tgcgccgacg
     8701 attttagcag cgctggagct gcgcgagttt ttcaccttct gcgcggacgc ttcccaactt
     8761 aaaaactcga aaccggaccc ggaaatcttt ctcgccgcct gtgccggact gggcgtgccg
     8821 ccgcaggcat gtatcggcat tgaagatgcg caggcgggca ttgacgccat taacgccagc
     8881 ggtatgcgct cggtggggat cggcgcgggc ttaaccgggg cgcaattatt gttgccctca
     8941 acggactccc tgacctggcc gcggttatcg gccttctggc aaaacgtata gcaaaggaat
     9001 caacatggct cagctttcgt tacaacatat tcaaaaaatc tacgataacc aggtgcatgt
     9061 ggtgaaggac ttcaacctgg aaattgccga taaagagttc attgtctttg tcggtccgtc
     9121 aggctgcggt aaatcgacta cgctgcgcat gattgccggg cttgaggaga tcagcggcgg
     9181 cgatctgttg atcgacggca aacgaatgaa tgacgttccg gctaaagagc gcaatatcgc
     9241 gatggtgttc ctgaactacg cgctgtatcc gcatatgacg gtctacgaca acatggcgtt
     9301 tggcctgaag atgcaaaaaa tcgccaacga ggtgattgat gagcgggtga actgggcggc
     9361 gcaaattctc ggcctgcgtg agtacctgaa acgcaagccg ggggcgcttt ccggcgggca
     9421 acgtcagcga gtggcgctcg ggcgggcgat tgtgcgcgaa gcgggcgtgt ttttaatgga
     9481 tgaaccgctc tctaacctgg atgccaagct gcgcgtgcaa atgcgcgcag agatcagcaa
     9541 gctgcatcag aaactgaaca ccaccatgat ctacgtgacc cacgatcaga ccgaagcgat
     9601 gaccatggcg acgcggattg tgattatgaa ggatgggatt gttcagcaag taggtgcgcc
     9661 gaaaacggtt tataaccaac cggcgaatat gtttgttgcc ggatttatag gatcaccggc
     9721 gatgaatttt attcgcggca cgattgatgg cgataaattc gttacggaaa cgcttaaatt
     9781 aaccatttcc gaggagaaat tagcggttct gaaaacacag gaaagtttgc ataagcccat
     9841 tgtgatggga atacgcccgg aagatattca tccggacgcg caagaggaaa ataacatttc
     9901 cgccaaaatt agcgtggcag aattaaccgg tgctgaattt atgctctaca ccacggttgg
     9961 ggggcacgaa ctggtggtcc gtgctggtgc gttaaatgat tatcatgcag gagaaaatat
    10021 cactattcat tttgatatga ctaaatgtca tttctttgat gcagaaacgg aaatagcaat
    10081 tcgctaaata cagggggaag gcattccccc aggataatac aaggaacaat aatgaaaaaa
    10141 ttattaccct gtaccgcact ggtgatgtgt gcgggaatgg cctgcgcaca ggccgaggaa
    10201 aagaacgact ggcactttaa tatcggcgcg atgtacgaaa tagaaaacgt cgagggttat
    10261 ggcgaagata tggatgggct ggcggagcct tctgtctatt ttaatgccgc caacgggccg
    10321 tggagaattt ctctggccta ttatcaggaa gggccggtag attatagcgc gggtaaacgt
    10381 ggaacgtggt ttgatcgccc ggagctggag gtacattatc agttcctcga aagcgatgat
    10441 ttcagtttcg gtctgactgg tggtttccgt aattatggtt atcactacgt tgatgaaccg
    10501 ggtaaagaca cggcgaatat gcagcgctgg aaaattgcgc cagactggga tgtgaaactg
    10561 actgacgatt tacgtttcaa cggttggctg tcgatgtata aatttgccaa cgatctgaat
    10621 acgaccggtt atgctgatac ccgtgtcgaa acggaaacgg gtctgcaata taccttcaac
    10681 gaaacggttg ccttgcgagt gaactattat ctcgagcgcg gtttcaatat ggatgatagc
    10741 cgcaataacg gtgagttttc cacgcaagaa attcgcgcct atttgccgct gacgctcggc
    10801 aaccactcgg tgacgccgta tacgcgcatt gggctggatc gctggagtaa ctgggactgg
    10861 caggatgata ttgaacgtga aggccatgat tttaaccgtg tggggttatt ttacggttat
    10921 gatttccaga atggtctttc cgtttcgctg gaatacgcgt ttgagtggca ggatcacgac
    10981 gaaggcgaca gcgataaatt ccattatgca ggtgtcggcg taaattactc gttctgataa
    11041 tgggttaaat tgccggatgc ggcgcgagcg ccaaatccgg catacattta tttcaacgac
    11101 atatgcgtgg caacggtaat attttgtggt gacggttttc cggcaattaa gcggaataat
    11161 aactcgcagc tttgttgccc taactcctgc gtcggaacat cgatgccgcc cggtgcaggc
    11221 gttaaaataa atgacagcgt ttcattgcta tagcccacca ccgctaactg ctgcggaata
    11281 gcaatatttt tctctgctgc cgcacgataa atgctcatta atttcaggct gtcagtggca
    11341 aatacggctt ctggcaacgg cgactggctt aataattgcc gtgcggcttg caaggctgtt
    11401 tcatgggtat aaccgccgtc aacaatccat tcatcacgca ctgcaatatt atgcgcagcc
    11461 agactttgct tatagccatt aacgcggtca accgagacat ggacatcaag cggagcatgc
    11521 aggcaggcaa tattttgatt cccactttca attaacgcat cggtcaacgc aatgctgtcg
    11581 ccaaaattat cggtatcgac agaataaaca tgggcatatt gaccttcaac tttgccaatt
    11641 accaccaccg gaatatcata tttatcgagt tgggcaaaaa atgactcatc cgctggcgaa
    11701 cttagcataa taatgccttt aatcattttc tgcttaattt tgctttcgca tttttgtaag
    11761 tcttctgcca ggttgtgcga cgtctgcaat atcacgtcga aaccttcttc ttcagctttg
    11821 gcggtgatgg catgtaaaac ttccgagaaa aacggattac ccgccgtagt tttggtcgaa
    11881 cgggtagaaa tcaccataat ggcatcaaaa cccgaagagg tcagcgcgcg tgccagcttg
    11941 tttggttgat actgtaattc ttcaatggcc cgtaacactt tttcgcgcgc ttccggggag
    12001 atattggttt gcttattcag cacgcgtgat acggtggatt ttgatacgcc tgcaaccctg
    12061 gcaatatcat aaatagtagg ggacataggt caggaactcc gtccgaacgt gaatgttgca
    12121 catcttatgg agttcgcccg tcgatgacaa cacgggcagt tgataatcaa tggcctggcc
    12181 cccacattca tatccttacg aatgattttt ttctggtctt cagagcagca caggacagca
    12241 atgaagcgac ttaaaaatga acttaatgcg ctggtgaatc ggggtgtcga cagacatctg
    12301 cgcctcgctg taaccggact tagccgcagc ggcaaaacag cgtttatcac cgcgatggtt
    12361 aatcagttgc tcaatattca tgccggagca cgtttgccgc tattaagtgc ggtgcgtgaa
    12421 gaacgcctgc tgggcgtaaa acgcattccc cagcgtgact ttggcattcc gcgcttcacc
    12481 tatgatgaag gactggcgca gttatatggc gatcctcccg cctggccgac gccaacgcgc
    12541 ggcgtcagcg aaattcgcct ggcgctacgc tataaatcga acgattcgct gctgcgccac
    12601 tttaaggata cctccacgct gtatttggag attgtggatt accccggcga atggttgctc
    12661 gacctgccga tgctggcgca ggactattta agctggtcgc gccagatgac gggcttactc
    12721 aatggtcagc gcggcgaatg gtcggcgaaa tggcgaatga tgtgcgaagg gctggacccg
    12781 ctagcgcctg ccgacgaaaa ccggctggca gacattgccg ccgcgtggac cgattatctc
    12841 caccactgta aacagcaggg gctgcacttt attcagcctg ggcgctttgt cttgccggga
    12901 gatatggcag gtgcgcccgc gctgcaattc ttcccatggc cagatgtcga tgcctggggc
    12961 gagtctaaac tggcgcaggc cgataaacac accaacgccg gaatgttgcg cgagcgtttt
    13021 aattattact gcgagaaggt ggtgaaagga ttctataaaa atcacttcct gcgttttgac
    13081 cgccagattg tgctggtgga ctgcctgcaa ccgctcaaca gtggaccaca ggcatttaat
    13141 gatatgcgtc tggcgctgac gcagctgatg caaagtttcc actacgggca gcgtaccctg
    13201 ttccggcgtt tgttttcgcc ggttatcgat aagctattgt ttgctgccac taaagcggac
    13261 catgtgacca tcgatcagca cgctaatatg gtttcattac tgcaacaact gattcaggat
    13321 gcctggcaaa atgcagcgtt cgaagggatc agcatggact gcctggggct ggcgtcagtt
    13381 caggcgacca ccagcggcat tattgatgtt aacggtgaga aaatcccggc gctgcgcggt
    13441 aatcgactta gtgatggcgc accgctcact gtttatcctg gcgaagtccc cgcacgtttg
    13501 cctggtcagg cgttctggga taagcaaggc ttccagtttg aggcgtttcg tccgcaggtg
    13561 atggatgtcg acaaaccgct accgcatatt cgccttgatg ctgcgctgga atttttaata
    13621 ggagataaat tgcgatgacc gaaccgttaa aaccacgtat tgatttcgac ggtccactgg
    13681 aggtcgatca gaatccaaaa ttcagggcgc agcagacctt tgacgaaaat caggcgcaaa
    13741 attttgcccc ggccacgctc gacgaagcgc aggaagaaga ggggcaagtt gaagcggtga
    13801 tggacgcagc gttacgtccg aaacgcagcc tgtggcgcaa aatggtgatg ggcgggttgg
    13861 ctctgtttgg cgcaagcgtt gtcgggcagg gtgtacagtg gacaatgaac gcctggcaaa
    13921 cccaggactg ggtggcgctg ggtggatgtg ccgctggggc attgattatc ggcgctggcg
    13981 taggttctgt ggtaacagag tggcggcgct tatggcgctt gcgacagcgc gcccatgaac
    14041 gcgacgaagc gcgtgattta ttgcatagtc acggcacggg taaaggccgc gcattttgcg
    14101 aaaaactggc acagcaggcg ggcatcgatc agtcacatcc ggcgctgcaa cgctggtatg
    14161 cctcaatcca tgaaacgcaa aacgaccgtg aagtggtcag tttgtatgcg catttggtcc
    14221 agccagtttt agatgcccag gcgcggcgcg aaatcagccg ttcggcagcg gaatcaacat
    14281 tgatgattgc ggtcagccca ctggcgctgg tggatatggc ttttatcgcc tggcgcaatc
    14341 tgcgtttgat caatcgcatc gccacgctgt atggcattga actgggatat tacagccgtt
    14401 tgcgcctgtt taagctggta ttgctgaata tcgctttcgc cggagccagc gaactggtgc
    14461 gcgaagtggg gatggactgg atgtcgcaag atctcgctgc gcgtttgtct acccgggcag
    14521 ctcaggggat tggtgctgga cttctgacgg cacgactggg gattaaagct atggaacttt
    14581 gccgcccgct gccgtggatt gaagatgaca aacctcgcct cggggatttc cgtcgtcagc
    14641 ttatcggtca ggtgaaagag acgctgcaaa agggcaaaac gcccagcgaa aaataatgca
    14701 atatcgggtg ctgaccggat atctttacgc cgaagtgccc gtttttccgt ctttgtgtca
    14761 atcattgttg acagaaacct tcctgctatc caaatagtgt catatcatca tattaattgt
    14821 tcttttttca ggtgaaggtt cccatgcgtc tggaagtctt ttgtgaagac cgacttggtc
    14881 tgacccgcga attactcgat ctactggtgc tgagaggcat tgatttacgc ggtattgaga
    14941 ttgaccccat tgggcgaatc tacctcaatt ttgccgaact ggaatttgaa agtttcagca
    15001 gtctgatggc cgaaatacgc cgtattgcag gtgttaccga tgtgcgcact gttccgtgga
    15061 tgccttccga acgtgagcat ctggcgttga gcgcgttact ggaggcgttg cctgagcctg
    15121 tgctttctgt tgatatgaaa agcaaagtgg atatggcgaa cccggcgagc tgtcagcttt
    15181 ttgggcaaaa attggatcgc ctgcgcaacc ataccgccgc gcaattaatt aacggcttta
    15241 attttttacg ctggctggaa agcgaaccgc aagactcgca taacgagcat gttgttatta
    15301 atgggcagaa tttcctgatg gagattacgc ctgtttatct tcaggatgaa aatgatcaac
    15361 atgttctgac cggtgcggtg gtgatgttgc gctcgacgat tcgtatgggc cgtcagttgc
    15421 agaatgtcgc tgcacaggac gtcagtgcct tcagtcaaat tgtcgccgtc agcccgaaaa
    15481 tgaagcatgt tgtcgaacag gcgcagaaac tggcgatgct aagcgcgccg ttgctgatta
    15541 cgggtgacac cggtacaggt aaagatctct ttgcctacgc ctgccatcag gcaagcccac
    15601 gagcgggcaa accttacctg gcgctgaact gcgcgtctat accggaagat gcggtcgaga
    15661 gtgaactgtt tggtcatgct ccggaaggta aaaaagggtt ctttgagcag gcgaacggtg
    15721 gttcggtgct gttagatgag ataggcgaga tgtcgccaag gatgcaggcg aaattactgc
    15781 gtttcctcaa tgatggtact ttccgtcgag ttggtgaaga tcatgaagtg catgtcgatg
    15841 taagggtgat ttgcgctacg cagaagaatt tggtcgaact ggtgcaaaaa ggcgtgttcc
    15901 gtgaagatct ctattatcgt ctgaacgtgt tgacgctcaa tctgccgccg ctacgtgact
    15961 gtccgcagga catcatgccg ttaaccgagc tgttcgtcgc ccgctttgcc gacgagcagg
    16021 gcgtgccgcg tccgaaactg gccgctgacc tgaatactgt acttacacgt tatgcgtggc
    16081 cgggaaatgt gcggcagtta aagaacgcta tctatcgcgc actgacacaa ctggacggtt
    16141 atgagctgcg tccacaggat attttgttgc cggattatga cgccgcaacg gtagccgtgg
    16201 gcgaagatgc gatggaaggt tcgctggacg aaatcaccag tcgttttgaa cgctcggtat
    16261 taacccagct ttatcgcaat tatcccagca cgcgcaaact ggcaaaacgt ctcggcgttt
    16321 cacataccgc gattgccaat aagttgcggg aatacggtct gagtcagaag aagaacgaag
    16381 agtaagcgcg aatatgcctg atggtgcaac accatcaggc atattaactt atgctttcag
    16441 tacagccaga gctgcttcgt aatccggctc ggtggtgatt tcatccacca gctggctgaa
    16501 aatcacattg tcattttcat caataaccac aacggcacgc gctgccagac ctttcagtgg
    16561 gccatcagca attgccacac cgtaggcttg cagaaattca gcgttacgga aagtggagag
    16621 ggtgataacg ttgttcagac cttctgcgcc gcagaaacga gactgggcga acggcagatc
    16681 ggcagagata cacagcacaa cggtgttgtc gatctctgtt gccagttggt taaacttacg
    16741 tactgatgcg gcgcaaacac cggtatcaat actcgggaaa atgttcagca ctttgcgttt
    16801 acccgcaaac tgaccgaggg tgacgtcaga cagatctttt gccacgagag taaaagtctg
    16861 cgctttgcta cccgcctgcg ggatggaatt ggcgactgta accgggttgc cctggaaatg
    16921 aacggtttgt gacatgatta tctttcctgt ttacatatag ttaacgtcac acctagttta
    16981 tgctaactgt caataacaca gcaaacgcta tttgcgctta atccgcagac caccgcgaca
    17041 acaaggagta aagatgagaa ccgttaaggt attcgaggaa ggctggccct tacatacccc
    17101 gtttgtgatt gctcggggaa gtcgcagtga agcgcgcgtg gtggtggttg aactggaaga
    17161 agaaggtatt aaaggcaccg gtgaatgcac gccgtatccg cgttatggag aaagtgatgc
    17221 ctcggtaatg gcgcaaatta tgagcgtcgt gccgcaatta gagaaagggc tgacacggga
    17281 ggagttgcaa aaaatcctcc ctgccggcgc agcacgtaat gcgctggatt gtgcattgtg
    17341 ggatctcgcg gcgcgaaaac agcaggaatc gctggctgat ttgatcggca taacgcttcc
    17401 cgggacagtt atcactgcac agacggttgt catcggtacg cctgatcaga tggccaatag
    17461 tgcatcaaca ctctggcagg caggcgcgaa attactgaaa gtgaagctgg ataaccatct
    17521 tatcagtgag cggatggtgg caattcgcac cgctgtgccc gatgcgacgc tgatcgttga
    17581 tgcaaatgaa tcctggcgtg cagaagggtt ggcggcgcgt tgccagctat tggcggattt
    17641 aggcgttgca atgcttgaac aaccgcttcc tgcgcaggac gatgcggcgt tggagaattt
    17701 tattcatccg ttaccgattt gtgctgatga aagttgtcat actcgtagca atctgaaggc
    17761 gctgaaaggg cgctatgaga tggttaacat taagctcgat aaaacggggg gcctgacgga
    17821 agcgctggcg ctggcgactg aagcacgtgc acaaggtttt agtctgatgc tgggctgcat
    17881 gttgtgtacc tctcgtgcca ttagcgccgc tttaccgctg gtgccgcagg tcagtttcgc
    17941 cgatcttgac ggaccgacct ggctggcggt agatgtggaa ccggcgcttc agttcacaac
    18001 gggcgaattg catctttagg atgccagcgc agcaagtttg ccatcgcaaa gagatatttt
    18061 tcgctggctt cgtcggagga gatgggtgga aactctgcgg tgatgcaatg caggttgaga
    18121 tccgcacacc aactgccgaa agagccaggc gtttcataac cgacgctggt taccagaggc
    18181 aattcaaacg cctgggccag ccattcacct aattcgctgt gtctgggatc ttcaatacag
    18241 gccagcggat cgtggaatga gaccacccag gcgggttgaa tgcggtgtat aagctggcac
    18301 agcgcctggg tttcaggttc ggagccaggt ttgtcacctg tcagtaaaac gacatcacgt
    18361 tcttcagcgg cgctgttcca gcgataaacg gtttcacctt ccttccagtt tgccgccgga
    18421 aagtttctgt ttaaatccac accattagca ttggccctta accccaactg gcagccgtca
    18481 ggattcacac acagcaccac atgatgacgg cgcaaagaag gtgtcaacgt ccgcagagca
    18541 caggagagcg tgacgatcga agagttttca tcaccgtgag tgccagccag aatcaaacca
    18601 ctttcgtgac tggctgcagg ggccggaaac cagattaacg gcgcgcccaa taatgaacgt
    18661 ccgtaatgtt ctgttccggg cggaaatgcg ccgcgttcgg cgcgtgggcg agttacggtc
    18721 atgatcgtct ctggttcagg aagtattaca ggcagtgttg tgcaaatttt gcggattatc
    18781 aaatcctttt cttcaacgct atgttatcga tcttgtatca cttttttgcc ggaagttgtt
    18841 tgcattttca ttgatgtgca gatattagaa ttgtcgtaat gttatataca tggcgtattt
    18901 tattcttact ggttctacac atgttaatcg catctgattg atcagttcac cttaataagt
    18961 ttaataaatc gtactgcaca tagtacagta cgatggtagc tatataattg atttacatga
    19021 agaatggctt atcgccatca gtcccgggtt gattgattcc tatattctca ccaatgtgtt
    19081 tctcaggttt atcgatgata tctgtaatta atgctgcgac actcttacgt gatacgatcg
    19141 tacctttgaa tggttcattg cgtgaggtaa gctcataatc aataatatct tcatcagtca
    19201 gccaggcagg gcgaagaatt gtatattcca gtccagaagc ctcaattgca tctgcagccc
    19261 tgcggaatgg ttttaggggt tcaccaataa ctgcattatt ccattcgcca aattttcctg
    19321 gaacttcatc ataaataccg agggaaagaa caaagatcag acgtttaaca tcaccggctt
    19381 tcatcgcagc gatcactgaa tttgcctgaa tatctaaatc ttcccccgta aggttggcat
    19441 acacaacatc ctgtccctgc atggcctgct tcagtgctgc atgattcagt acatccccca
    19501 taataatctt gctattagtg ggatagggtt tgtggatttt tgctggctga cgggcaaata
    19561 aagtttgttt aatggtttgt ttatctgcca gttgattaat cacatggcgg gctatctgac
    19621 cgccagcacc aagaatcagt acatttttca tgaaactttt cccagattat tggcagcaat
    19681 atgccaggcc atatcactat tacggatttg aatatgtttc attacagagt ttcctcaaag
    19741 aacggagcta atactgaaat tgcttcagcc acatagactt tgccgtcata aagatccata
    19801 tggtttgcgc cttcaacaat gtgatagcgt ttatcctggc ttgatgctcg atcgtacagg
    19861 tcgtcactca tccatttgct ccctgcctgg ctgcccgcca caatctgtat cggctgagtc
    19921 aggtacactt ccgccatatg gtaagcatca taggtaataa tctggttaag gctgcgcaaa
    19981 gtagcgtaac ccggtgctgt tggatactgc gcgcgagggg tatggtaata ctcccaggcc
    20041 tgacgcagtt cttcgttcgg cgcatcggac tccttcattg gtgccagtgg catagtggcg
    20101 tattctctgc tgctaatatc gctggttcgg gcgtttgaac ccgcgtcaac gtatggtagg
    20161 gcatctatag atttcacatt gttttcccaa ccattacgga acatcgaacc aatattgacc
    20221 gcactaacag taccgatggc cttgatgcgg cgatcctgaa ttgcagcatt ggctgtatat
    20281 cctgcaccgg cacaaattcc catcgcacca attcggctat tgtcgacata agaaagcgtt
    20341 gtcaggtaat caatcacggc actgacgtct tcagtacgaa tgtatgggtt ttctaactga
    20401 cgcggctcgc cgccgctttc accttgataa gatgcgtcat aagcaatagt gacaaatccc
    20461 ttttccgcca gtttttcggc ataggttcca gccgtttgct ctttaacgcc cccacctggg
    20521 tgagatacca caattgccgg atactgacgg gtttcatcaa attttggagg gaaatagatc
    20581 actgcagaca aagagatggt tggattattg ctgttagtga agctgacttt attattcatc
    20641 attccgttcc tcatggagtt gtcggttcgt tttgacggtt ggtgatatca ctatagatat
    20701 tgatcattaa gttgattaga cccaaaatca tgattagacc tatcatttaa atgattaata
    20761 gattgtatgg agatactgaa tgaagcgtga agaaatcgct gatctgatgg cgtttgtcgt
    20821 cgttgcagag gagcgtagct tcactcgcgc agcagcccgc ctgagcatgg cgcagtcagc
    20881 tttaagccag atagtgcgcc gtatagaaga acgattggga ttgcggcttc tgacacgaac
    20941 cacgcgcagc gttgtaccca ctgaagcggg cgagcatctt ttgtctgttc ttggccctat
    21001 gttgcatgac atagattctg ccctgacatc cctgagcgat ctgcagaacc gcccatccgg
    21061 gacaatacgt attactactg tagaacatgc agcaaaaacg atattgttac cagctatgcg
    21121 cacattcctg aaatcgcatc ctgaaattga tatccagctt accattgatt atggtttgac
    21181 cgatgtcgtt tctgaacgtt ttgatgcagg cgtccgtctg ggtggggaga tggataaaga
    21241 tatgatcgcc attcgaatcg ggccagatat accaatggct attgttggct caccggatta
    21301 tttttctcgt cgaagtgctc caacctcagt gtcacaatta atagatcatc aggcaattaa
    21361 tttgtatctt cccacatcgg gtacagcaaa tcgctggaga ttaatacgcg gtggacgtga
    21421 agttcgtgtt cgcatggaag gtcagctttt actgaatacg atagacctga tcattgatgc
    21481 tgcaattgat gggcatggat tggcgtatct accttatgat caggttgagc gggctattaa
    21541 agaaaaaaaa ctgatacgtg ttttggataa attcacacca gatttacccg gttatcacct
    21601 gtactatcca caccgtcgac atgctggctc ggcattctca ttatttatag ataggctgaa
    21661 gtataaaggt gctgtttagc actacttgct gatacattaa tttaattctt ctcttaacgt
    21721 attctcagtt cctttcaacg ttttggttat tttttattct tcgtacaatg gcgacagatg
    21781 ctgattatga taccgaaaac gggtttgaac gtgcgaagcc cgaacgtagt gttcggagtt
    21841 ctatgtgctt taccgcattt tggagactat tatttacact aaatctgatt tgatatattg
    21901 atacttaaaa catttgatgc ttcctttgtc acttttttga tggaagttgt ttgcatttct
    21961 ttaaggcgaa acaaataatt acgcatcaat tttaatgtcg gttagaggga aacttatgaa
    22021 gcactctgtt tcagtcacgt gttgtgcgct gttggtcagc agcatttctc tttcgtatgc
    22081 tgctgaagtt ccgagcggca cagtactggc agagaagcag gagctggtgc gccacattaa
    22141 agatgagcct gcgtcgctgg atcccgctaa agccgtgggc ctgccagaga ttcaggtcat
    22201 tcgcgatctg tttgaaggac tggtgaatca gaacgagaaa ggggagattg tccccggcgt
    22261 tgcgactcag tggaaaagta atgacaaccg tatctggact tttaccctgc gcgataacgc
    22321 aaaatgggcg gatggcacac cggtaacggc gcaagatttt gtctacagct ggcaacgtct
    22381 ggtggaccca aaaacattgt cgccatttgc atggtttgcc gcgctggcgg gaatcaacaa
    22441 cgcacaggcg attattgatg gtaaagccgc gcctgaccag cttggcgtca ccgcagttga
    22501 tgcccaaact ttgaaaatcc agcttgataa accgttgccg tggtttgtga atttaaccgc
    22561 taactttgcc ttcttcccgg tgcaaaaagc caacgtagaa agcagtaaag agtggacgaa
    22621 acccggaaat ctgatcggta atggcgctta tgttcttaaa gagcgcgtag tcaatgaaaa
    22681 actggtcgtg gtaccgaata cccattattg ggataacgcc aaaacggtgc tgcaaaaagt
    22741 gaccttcctg ccaattaatc aggaatccgc agccactaag cgttatctcg cgggggatat
    22801 tgatatcacc gaatccttcc cgaaaaatat gtatcagaag ctgttgaagg atattccggg
    22861 gcaggtttat acgccgccac agctcgggac ctattattat gcgtttaaca cgcaaaaagg
    22921 gccgacggcc gatcagcgtg ttcgcctggc attaagtatg acgatagatc gccgcctgat
    22981 gaccgaaaaa gtgttgggaa cgggcgaaaa gccagcgtgg cattttacac cagatgttac
    23041 tgcaggattt acgccggaac cttcgccgtt tgaacaaatg agtcaggaag aactgaatgc
    23101 gcaggcaaaa actttgttga gcgcagctgg ttatggtccg caaaaaccgc tgaagctgac
    23161 gcttttgtat aacacttcag aaaaccatca aaaaattgcg attgctgtag catcaatgtg
    23221 gaaaaagaac cttggcgtag atgttaaatt gcaaaatcag gaatggaaaa cctatatcga
    23281 tagccgtaac accggcaatt ttgatgttat ccgagcctcg tgggtagggg attataatga
    23341 accctccact ttcctgacat tattaacgtc aacgcattca ggaaatattt cacgctttaa
    23401 caatccggta tacgacaaag tactggccca ggcatcaacg gaaaataccg ttaaagcgcg
    23461 taatgccgat tacaatacgg cagaaaaaat cctcatggag caagcaccga ttgcaccaat
    23521 ttatcaatat accaatggac gattaatcaa gccgtggctg aaaggttatc ccattaataa
    23581 cccggaagat gtggcgtaca gtcggactat gtatattgtg aagcattgat gtgatgggaa
    23641 ctggcgttac ccttgtggat aacgccagtg atgtctgatt tagcgaccct ggtccggcgg
    23701 tgtaatgtta tccatataca gcgtctggct ggggaaggca aagtccgcgc cgtgtgactg
    23761 tacaatatcg ataatcttca aataaacgtc ttgctgtgca gcaagccatt cagcccatac
    23821 cgtggttttg gtaaagcaat aaaccataat attcaatgaa gagtcagcaa actggttgaa
    23881 ataaaccagt aaggtttgtc gctggtcgat ggccgggtga tttttcagca tctcacgtac
    23941 ggcttcgaca ataacgccca cttttgccgc atcctcataa cgtaaaccaa tggtcgtggt
    24001 aatgcgccgg ttggtcattc gtcctgggtt ttctacgctg atcgacgaaa acagcgagtt
    24061 cggtacgtac aacggacgat tatcaaaagt cgtaattttg gtaattcgcc agccaatttc
    24121 cgctactgta ccttcgatat ttctgtccgg tgaacggatc cagtcgccaa tactaaaagg
    24181 acggtcgaaa tagagcataa tcccggaaaa gaagttactc aaaatatctt taccggccat
    24241 accgacagcc agaccaccaa taccaccaaa ggtcaacaag ccagaaaggc tcataccgaa
    24301 atgttcacca taaagcagaa caagcaccac aataatggtg attttgatga tacgcgacat
    24361 aatccgcgca ctggtgatat cgcgaccttt tttaatctgc tgtttttcaa actgatttat
    24421 cagcagaaat agcttaatcg tcagaataac cgcaatcagg gacgtacaga taaaat
//
LOCUS       DAZFLD010000059        24216 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.059, whole genome shotgun sequence.
ACCESSION   DAZFLD010000059 DAZFLD010000000
VERSION     DAZFLD010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24216)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24216)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24216
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.059"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            88..849
                     /gene="adiY"
                     /locus_tag="ACHKBL_001984"
     CDS             88..849
                     /gene="adiY"
                     /locus_tag="ACHKBL_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator AdiY"
                     /protein_id="HFO5818862.1"
                     /translation="MRICSDQPCIVLLTEKDVWIRVNGKEPISLKANHMALLNCENNI
                     IDVSSLNNTLVAHISHDIIKDYLRFLNKDLSQIPVWQRSATPILTLPCLTPDVFRVAA
                     QHSMMPAETESEKERTRALLFTVLSRFLDSKKFLSLMMYMLRNCVSDSVYQIIESDIH
                     KDWNLSMVASCLCLSPSLLKKKLKSENTSYSQIITTCRMRYAVNELMMDGKNISQVSQ
                     SCGYNSTSYFISVFKDFYGMTPLHYVSQHRERTVA"
     gene            986..2323
                     /gene="adiC"
                     /locus_tag="ACHKBL_001985"
     CDS             986..2323
                     /gene="adiC"
                     /locus_tag="ACHKBL_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/agmatine antiporter"
                     /protein_id="HFO5818863.1"
                     /translation="MSSDADAHKVGLIPVTLMVSGNIMGSGVFLLPANLASTGGIAIY
                     GWLVTIIGALGLSMVYAKMSFLDPSPGGSYAYARRCFGPFLGYQTNVLYWLACWIGNI
                     AMVVIGVGYLSYFFPILKDPLVLTITCVVVLWIFVLLNIVGPKMITRVQAVATVLALI
                     PIVGIAVFGWFWFRGETYMAAWNVSGLGTFGAIQSTLNVTLWSFIGVESASVAAGVVK
                     NPKRNVPIATIGGVLIAAVCYVLSTTAIMGMIPNAALRVSASPFGDAARMALGDTAGA
                     IVSFCAAAGCLGSLGGWTLLAGQTAKAAADDGLFPPIFARVNKAGTPVAGLIIVGILM
                     TIFQLSSISPNATKEFGLVSSVSVIFTLVPYLYTCAALLLLGHGHFGKARPAYLAVTT
                     IAFLYCIWAVVGSGAKEVMWSFVTLMVITAMYALNYNRLHKNPYPLDAPISKD"
     gene            2427..4070
                     /gene="eptA"
                     /locus_tag="ACHKBL_001986"
     CDS             2427..4070
                     /gene="eptA"
                     /locus_tag="ACHKBL_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase EptA"
                     /protein_id="HFO5818864.1"
                     /translation="MLKRLLKRPSLNLFSWLLLATFYISVCLNIAFFKQVLQVLPLDS
                     LHNVLVFLSMPVVAFSVINIVLTLGSFLWLNRPLACLFILVGAAAQYFIMTYGIVIDR
                     SMITNIIDTTPAESYALMTPRMLLTLGLSGVLAAIIACWIKITPTTSRLRSVLFRGAN
                     ILISVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRSANLPLIR
                     IGEDAHRNPLMQNGKRKNLTILIVGETSRAENFSLNGYPRETNPRLAKDNVVYFPNTA
                     SCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDIIQRAGINVLWNDNDGGCKGVCD
                     RVPHQNVTALNLPGQCINGECYDEVLFHGLEEYINNLQGDGVIVLHTIGSHGPTYYNR
                     YPPQFRKFTPTCDTNEIQTCTKEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSL
                     VYLSDHGESLGENGIYLHGLPYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQ
                     TQHYSQDNLFSTLLGLTGVETKYYQAADDILQTCRRVSE"
     gene            4067..4735
                     /gene="basR"
                     /locus_tag="ACHKBL_001987"
     CDS             4067..4735
                     /gene="basR"
                     /locus_tag="ACHKBL_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BasR"
                     /protein_id="HFO5818865.1"
                     /translation="MKILIVEDDTLLLQGLILAAQTEGYACDGVTTARMAEQSLEAGH
                     YSLVVLDLGLPDEDGLHFLARIRQKKYTLPVLILTARDTLTDKIAGLDVGADDYLVKP
                     FALEELHARIRALLRRHNNQGESELIVGNLTLNMGRRQVWMGGEELILTPKEYALLSR
                     LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKARIRTVRGFGYMLVA
                     NEEN"
     gene            4736..5836
                     /gene="pmrB"
                     /locus_tag="ACHKBL_001988"
     CDS             4736..5836
                     /gene="pmrB"
                     /locus_tag="ACHKBL_001988"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PmrB"
                     /protein_id="HFO5818866.1"
                     /translation="MNLMRFLRRPISLRQRLILTIGAILLVFELISVFWLWHESTEQI
                     QLFEQALRDNRNNDRHIMREIREAVASLIVPGVFMVSLTLFICYQAVRRITRPLAELQ
                     KELEARTADNLTPIAIHSATLEIEAVVSALNDLVSRLTSTLDNERLFTADVAHELRTP
                     LAGVRLHLELLAKTHHIDVAPLVARLDQMMESVSQLLQLARAGQSFSSGNYQHVKLLE
                     DVILPSYDELSTMLDQRQQNLLLPESAADITVQGDATLLRMLLRNLVENAHRYSPQGS
                     NIMIKLQEDGGAVMAVEDEGPGIDESKCGELSKAFVRMDSRYGGIGLGLSIVSRITQL
                     HHGQFFLQNRQETSGTRAWVRLKKDQNVANQI"
     gene            complement(5812..5901)
                     /gene="pmrR"
                     /locus_tag="ACHKBL_001989"
     CDS             complement(5812..5901)
                     /gene="pmrR"
                     /locus_tag="ACHKBL_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxT activity modulator PmrR"
                     /protein_id="HFO5818867.1"
                     /translation="MKNRVYESLTTVFSVLVVSSFLYIWFATF"
     gene            complement(6013..7515)
                     /gene="proP"
                     /locus_tag="ACHKBL_001990"
     CDS             complement(6013..7515)
                     /gene="proP"
                     /locus_tag="ACHKBL_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline transporter ProP"
                     /protein_id="HFO5818868.1"
                     /translation="MLKRKKVKPITLRDVTIIDDGKLRKAITAASLGNAMEWFDFGVY
                     GFVAYALGKVFFPGADPSVQMVAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAIT
                     IVIMSISTFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGEYTGASIFVAEYSPDRK
                     RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEANFLDWGWRIPFFIALPLGIIGLYLR
                     HALEETPAFQQHVDKLEQGDREGLQDGPKVSFKEIATKHWRSLLTCIGLVIATNVTYY
                     MLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSDRFGRRPFVLLGSVA
                     LFVLAIPAFILINSNVIGLIFAGLLMLAVILNCFTGVMASTLPAMFPTHIRYSALAAA
                     FNISVLVAGLTPTLAAWLVESSQNLMMPAYYLMVVAVVGLITGVTMKETANRPLKGAT
                     PAASDIQEAKEILVEHYDNIEQKIDDIDHEIADLQAKRTRLVQQHPRIDE"
     gene            complement(7779..8657)
                     /gene="rdcB"
                     /locus_tag="ACHKBL_001991"
     CDS             complement(7779..8657)
                     /gene="rdcB"
                     /locus_tag="ACHKBL_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase regulator RdcB family
                     protein"
                     /protein_id="HFO5818869.1"
                     /translation="MTKTLLDGPGRVLESIHPRFLVDLAQGDDARHPQAHQQQFRERL
                     MQELLARVQLQAWTNGGMLNAPLSLRLTLVEKLASMLDPGHLALTQIAQHLALLQKMD
                     HRQHSAFPELPQQIAALYEWFSARCRWKEKALTQRGLLVQAGEQSEQIFTRWRAGAYN
                     AWSLPGRCFIVREELRWGAFGDAFRLGSPQAVALLLGDLRVKATQHLAESINAAPTTR
                     HYYHQWFASSTIPTGGDHADFLSWLGKWSTADKQPVCWSVTQRWRTVALGMPRLCSAQ
                     RLAGAMVEEIFSVNLV"
     gene            complement(8654..10883)
                     /gene="crfC"
                     /locus_tag="ACHKBL_001992"
                     /pseudo
     CDS             complement(8654..10883)
                     /gene="crfC"
                     /locus_tag="ACHKBL_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418533.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="clamp-binding protein CrfC"
     gene            11285..11620
                     /gene="yjdM"
                     /locus_tag="ACHKBL_001993"
     CDS             11285..11620
                     /gene="yjdM"
                     /locus_tag="ACHKBL_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HFO5818870.1"
                     /translation="MSLPHCPKCNSEYTYEDNGMYICPECAYEWNDAEPAQESDELIV
                     KDANGNLLADGDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCKIDGFGPMK
                     LKSEFVKKN"
     gene            11780..12223
                     /gene="yjdN"
                     /locus_tag="ACHKBL_001994"
     CDS             11780..12223
                     /gene="yjdN"
                     /locus_tag="ACHKBL_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family metalloprotein YjdN"
                     /protein_id="HFO5818871.1"
                     /translation="MPLSPYLSFAGNCSDAIAYYQRTLGAELLYKISFGEMPKSAQDS
                     AENCPSGMQFPDTAIAHANVRIAGSDIMMSDAIPSGKASYSGFTLVLDSQQVEEGKRW
                     FDNLAANGKIEMAWQETFWAHGFGKVTDKFGVPWMINVVKQQPTH"
     gene            12356..13144
                     /gene="phnC"
                     /locus_tag="ACHKBL_001995"
     CDS             12356..13144
                     /gene="phnC"
                     /locus_tag="ACHKBL_001995"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HFO5818872.1"
                     /translation="MQTIIRVEKLAKTFNQHQALHAVDLNIHHGEMVALLGPSGSGKS
                     TLLRHLSGLITGDKSAGSHIELLGRTVQREGRLARDIRKSRANTGYIFQQFNLVNRLS
                     VLENVLIGALGSTPFWRTCFSYFTREQKQRALQALTRVGMVHFAHQRVSTLSGGQQQR
                     VAIARALMQQAKVILADEPIASLDPESARIVMDTLRDINQNDGITVVVTLHQVDYALR
                     YCERIVALRQGHVFYDGSSQQFDNERFDHLYRSINRVEENAKAA"
     gene            13169..14185
                     /gene="phnD"
                     /locus_tag="ACHKBL_001996"
     CDS             13169..14185
                     /gene="phnD"
                     /locus_tag="ACHKBL_001996"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO5818873.1"
                     /translation="MNAKIIASLTFTSMFSLSTLLSPAHAEEQEKALNFGIISTESQQ
                     NLKPQWTPFLQDMEKKLGVKVNAFFAPDYAGIIQGMRFNKVDIAWYGNLSAMEAVDRA
                     NGQVFAQTVAADGSPGYWSVLIVNKDSPINNLNDLLAKRKDLTFGNGDPNSTSGFLVP
                     GYYVFAKNNISASDFKRTVNAGHETNALAVANKQVDVATNNTENLDKLKTSAPEKLKE
                     LKVIWKSPLIPGDPIVWRKNLSETTKDKIYDFFMNYGKTPEEKAVLERLGWAPFRASS
                     DLQLVPIRQLALFKEMQGVKGNKGLNEQDKLAKTSAIQAQLDDLDRLNNALSAMSSVS
                     KAVQ"
     gene            14291..15070
                     /gene="phnE"
                     /locus_tag="ACHKBL_001997"
     CDS             14291..15070
                     /gene="phnE"
                     /locus_tag="ACHKBL_001997"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HFO5818874.1"
                     /translation="MQTITIAPPKRSWFSLLSWAIVLAVLVVSWQGAEMAPLTLIKDG
                     GNMATFAADFFPPDFSQWQDYLTEMAVTLQIAVWGTALAVVLSIPFGLMSAENLVPWW
                     VYQPVRRLMDACRAINEMVFAMLFVVAVGLGPFAGVLALFIHTTGVLSKLLSEAVEAI
                     EPGPVEGIRATGANKLEEILYGVLPQVMPLLISYSLYRFESNVRSATVVGMVGAGGIG
                     VTLWEAIRGFQFQQTCALMVLIIVTVSLLDFLSQRLRKHFI"
     gene            15091..15816
                     /gene="phnF"
                     /locus_tag="ACHKBL_001998"
     CDS             15091..15816
                     /gene="phnF"
                     /locus_tag="ACHKBL_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism transcriptional regulator
                     PhnF"
                     /protein_id="HFO5818875.1"
                     /translation="MHLSTHPTSYPTRYQEIAAKLEQELRQHYRCGDYLPAEQQLAAR
                     FEVNRHTLRRAIDQLVEKGWVQRRQGVGVLVLMRPFDYPLNAQARFSQNLLDQGSHPT
                     SEKLLSVLRPASGHVADALGITEGENVIHLRTLRRVNGVALCLIDHYFADLTLWPTLQ
                     HFDSGSLHDFLREQTGITLRRSQTRISARRAQAKECQRLEIPNLSPLLCVRTLNHRDG
                     ESSPAEYSVSLTRADMIEFTMEH"
     gene            15817..16269
                     /gene="phnG"
                     /locus_tag="ACHKBL_001999"
     CDS             15817..16269
                     /gene="phnG"
                     /locus_tag="ACHKBL_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HFO5818876.1"
                     /translation="MHADTATRQHWMSVLAHSQPTELAARLNALNITADYEVIRAAET
                     GLVQIQARMGGTGERFFAGDATLTRAAVRLTDGTLGYSWVLGRDKQHAERCALIDALM
                     QQTRHFQNLSETLIAPLNADRMARIAARQAEVNASRVDFFTMVRGDNA"
     gene            16266..16850
                     /gene="phnH"
                     /locus_tag="ACHKBL_002000"
     CDS             16266..16850
                     /gene="phnH"
                     /locus_tag="ACHKBL_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HFO5818877.1"
                     /translation="MTLETAFMLPVQDAQHSFRRLLKAMSEPGVIVALHQLKRGWQPL
                     NIATTSVLLTLADNDTPVWLSAPLSNDIVSQSLRFHTNAPLVNQPELATFAVTDEAIS
                     SEQLNALSSGTAVAPEAGATLILQVVSLSGGRMLRLTGAGIAEERMIAPQLPECILHE
                     LTERPHPFPLGIDLILTCGERLLAIPRTTHVEVC"
     gene            16850..17914
                     /gene="phnI"
                     /locus_tag="ACHKBL_002001"
     CDS             16850..17914
                     /gene="phnI"
                     /locus_tag="ACHKBL_002001"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate synthase subunit PhnI"
                     /protein_id="HFO5818878.1"
                     /translation="MYVAVKGGEKAIDAAHALQESRRRGNTDLPELSVAQIEQQLNLA
                     VDRVMTEGGIADRELAALALKQASGDNVEAIFLLRAYRTTLAKLAVSEPLDTTEMRLE
                     RRISAVYKDIPGGQLLGPTYDYTHRLLDFTLLANGEAPTLTTADSEQQPSPHVFSLLA
                     RQGLAKFEEDSGAQPDDITRTPPVYPCSRSSRLQQLMRGDEGYLLALAYSTQRGYGRN
                     HPFAGEIRSGYIDVSIVQEELGFAVNVGELLMTECEMVNGFIDPPDEPPHFTRGYGLV
                     FGMSERKAMAMALVDRALQAPEYGEHATGPAQDEEFVLAHADNVEAAGFVSHLKLPHY
                     VDFQAELELLKRLQQEQNHG"
     gene            17907..18752
                     /gene="phnJ"
                     /locus_tag="ACHKBL_002002"
     CDS             17907..18752
                     /gene="phnJ"
                     /locus_tag="ACHKBL_002002"
                     /EC_number="4.7.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate 5-phosphate
                     C-P-lyase PhnJ"
                     /protein_id="HFO5818879.1"
                     /translation="MANLSGYNFAYLDEQTKRMIRRAILKAVAIPGYQVPFGGREMPM
                     PYGWGTGGIQLTASVIGESDVLKVIDQGADDTTNAVSIRNFFKRVTGVNTTERTDDAT
                     LIQTRHRIPETPLTEDQIIIFQVPIPEPLRFIEPRETETRTMHALEEYGVMQVKLYED
                     IARFGHIATTYAYPVKVNGRYVMDPSPIPKFDNPKMNMMPALQLFGAGREKRIYAVPP
                     FTRVESLDFDDHPFTVQQWNEPCAICGSTHSYLDEVVLDDAGNRMFVCSDTDYCRQQS
                     EAKSQ"
     gene            18749..19507
                     /gene="phnK"
                     /locus_tag="ACHKBL_002003"
     CDS             18749..19507
                     /gene="phnK"
                     /locus_tag="ACHKBL_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018835 - carbon phosphorus lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnK"
                     /protein_id="HFO5818880.1"
                     /translation="MNQPLLSVNNLTHLYAPGKGFSDVSFDLWPGEVLGIVGESGSGK
                     TTLLKSISARLTPQQGEIRYENRSLYGMSEADRRRLLRTEWGVVHQHPLDGLRRQVSA
                     GGNIGERLMATGARHYGDIRATAQKWLEEVEIPANRIDDLPTTFSGGMQQRLQIARNL
                     VTHPKLVFMDEPTGGLDVSVQARLLDLLRGLVVELNLAVVIVTHDLGVARLLADRLLV
                     MKQGQVVESGLTDRVLDDPHHPYTQLLVSSVLQN"
     gene            19618..20298
                     /gene="phnL"
                     /locus_tag="ACHKBL_002004"
     CDS             19618..20298
                     /gene="phnL"
                     /locus_tag="ACHKBL_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018835 - carbon phosphorus lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnL"
                     /protein_id="HFO5818881.1"
                     /translation="MINVQNVSKTFILHQQNGVRLPVLQRASLTVNAGECVVLHGHSG
                     SGKSTLLRSLYANYLPDEGQIQIKHGEEWVDLVTAPARKVVEIRKTTIGWVSQFLRVI
                     PRISALEVVMQPLLDTGVPREACAAKAARLLTRLNVPERLWHLAPSTFSGGEQQRVNI
                     ARGFIVDYPILLLDEPTASLDAKNSAAVVELIREAKARGAAIVGIFHDEAVRNDVADR
                     LHPMGVSS"
     gene            20295..21431
                     /gene="phnM"
                     /locus_tag="ACHKBL_002005"
     CDS             20295..21431
                     /gene="phnM"
                     /locus_tag="ACHKBL_002005"
                     /EC_number="3.6.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HFO5818882.1"
                     /translation="MIINNVKLVLENEVVHGSLEVQDGKIRAFAESQSRQPEAMDGEG
                     GWLLPGLIELHTDNLDKFFTPRPKVDWPAHSAMSSHDALMVASGITTVLDAVAIGDVR
                     DGGDRLENLEKMINAIEETQKRGVNRAEHRLHLRCELPHHTTLPLFEKLVQREPVTLV
                     SLMDHSPGQRQFANREKYREYYQGKYSLTEAQMQQYEEEQLALAARWSQPNRETIAAM
                     CRARHIALASHDDATHAHVAESHQLGSVIAEFPTTFEAAEASRKHGMNVLMGAPNIVR
                     GGSHSGNVAASELAQLGLLDILSSDYYPASLLDAAFRVADDESNRFTLPQAVKLVTKN
                     PAQALNLQDRGVIGEGKRADLVLAHRKGNHIHIDHVWRQGKRVF"
     gene            21431..21988
                     /gene="phnN"
                     /locus_tag="ACHKBL_002006"
     CDS             21431..21988
                     /gene="phnN"
                     /locus_tag="ACHKBL_002006"
                     /EC_number="2.7.4.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033863 - ribose 1,5-bisphosphate
                     phosphokinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 1,5-bisphosphokinase"
                     /protein_id="HFO5818883.1"
                     /translation="MTGKLIWLMGASGSGKDSLLTELRQREQTQLLVAHRYITRAASA
                     GSENHIALSEQEFFTRAGQNLLALSWHANGLYYGVGVEIDLWLHAGFDVVVNGSRAHL
                     PQARARYQSALLPVCLQVSPEILRQRLENRGRENASEINARLARAARYTPQDCLTLNN
                     DGSLRQSVDKLLTLIHQKEKHHACL"
     gene            21975..22409
                     /gene="phnO"
                     /locus_tag="ACHKBL_002007"
     CDS             21975..22409
                     /gene="phnO"
                     /locus_tag="ACHKBL_002007"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoalkylphosphonate N-acetyltransferase"
                     /protein_id="HFO5818884.1"
                     /translation="MPACELRPATQYDTDAVYALICELKQAEFDHQAFRVGFNANLRD
                     PNMRYQLALLDGEVVGMIGLHLQFHLHHVNWIGEIQELVVMPQARGLNVGSKLLAWAE
                     EEARQAGAEMTELSTNVKRHDAHRFYLREGYEQSHFRFTKVL"
     gene            22411..23169
                     /gene="phnP"
                     /locus_tag="ACHKBL_002008"
     CDS             22411..23169
                     /gene="phnP"
                     /locus_tag="ACHKBL_002008"
                     /EC_number="3.1.4.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism protein PhnP"
                     /protein_id="HFO5818885.1"
                     /translation="MSLTLTLTGTGGAQGVPAWGCECVACVRARRSPQYRRQPCSGVV
                     KFNDAITLIDAGRHDLADRWSPGSFQQFLLTHYHMDHVQGLFPLRWGVGDQIPVYGPP
                     DEQGCDDLFKHSGLLDFSHTVEPFVMFDLQGLQVTPLPLNHSKLTFGYLLETAHSRVA
                     WLSDTAGLPEKTLKFLRNNQPQVMVIDCSHPPREDAPRNHCDLNTVLALNQVIRSPRV
                     ILTHISHQFDAWLMENALPSGFEAGFDGMEIEVA"
     gene            complement(23197..23526)
                     /gene="yjdP"
                     /locus_tag="ACHKBL_002009"
     CDS             complement(23197..23526)
                     /gene="yjdP"
                     /locus_tag="ACHKBL_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDRRRQL repeat protein YjdP"
                     /protein_id="HFO5818886.1"
                     /translation="MKRFPLFLLFTLLTLSTVPAQADIIDDTIGNIQQAINDAYNPDR
                     GRDYEDSRDDGWQREVSDDRRRQYDDRRRQFEDRRRQLDDRQRQLDQERRQLEDEERR
                     MEDEYGR"
     gene            complement(23595..24044)
                     /gene="rpiB"
                     /locus_tag="ACHKBL_002010"
                     /gene_synonym="alsI"
     CDS             complement(23595..24044)
                     /gene="rpiB"
                     /locus_tag="ACHKBL_002010"
                     /gene_synonym="alsI"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional allose-6-phosphate
                     isomerase/ribose-5-phosphate isomerase RpiB"
                     /protein_id="HFO5818887.1"
                     /translation="MKKIAFGCDHVGFILKHEIVAHLVERGVEVIDKGTWSSERTDYP
                     HYASHVALAVAGGEADGGILICGTGVGISIAANKFAGIRAVVCSEPYSAQLSRQHNDT
                     NVLAFGSRVVGLELAKMIVDAWLGAQYEGGRHQQRVEAITAIEQRRN"
BASE COUNT         5739 a         6823 c         6345 g         5309 t
ORIGIN      
        1 tattcatcat acaattatat aaccatttcc cggtatcgct ttgctttagc gagaactggt
       61 cttttattcg cactcaggag tacatgtatg aggatttgca gcgaccaacc ttgtattgtt
      121 ttattgactg aaaaagatgt ctggataagg gtgaatggga aagaacctat tagccttaaa
      181 gctaaccata tggcgttatt aaattgtgaa aataatatta tcgacgtctc ctctcttaat
      241 aacactttgg ttgctcatat tagtcacgac atcatcaaag attacctccg gtttctgaat
      301 aaagatctct cgcaaatacc agtatggcaa cgtagcgcta cgcccatcct cacccttcca
      361 tgcctgacgc cagacgtctt tcgcgttgcc gcgcaacata gcatgatgcc cgctgaaact
      421 gagtcagaaa aggaacgaac acgtgcatta ttattcactg tgctatcccg ttttctcgac
      481 agtaaaaaat tcctttcact aatgatgtat atgttacgta attgtgtaag tgacagcgtt
      541 tatcaaatta ttgaaagcga tatccacaaa gactggaatc ttagtatggt agccagttgt
      601 ttatgtctta gcccaagtct gttaaagaaa aagctgaaaa gcgaaaacac cagttatagc
      661 caaataatca ccacctgccg catgcgctat gccgtaaatg aattaatgat ggacggtaaa
      721 aatatctctc aggtatcaca gtcctgcggc tacaacagta cgtcgtactt tatttctgtc
      781 tttaaagact tctacggtat gacgccgctg cattatgtta gtcagcacag agaacgcact
      841 gtcgcctgat ttttaacctt aacgaagagc tatattaata acggcatcag cgataacccg
      901 gccgataata attcaactat cgaatgcagg cgtatgatac gacgtaattt attgtcacga
      961 agctcgcctt cgcaggagtt taattatgtc ttcggatgct gatgctcaca aagtgggctt
     1021 aatccccgtc accctgatgg tgtcggggaa tattatgggg tcaggtgttt ttctgttacc
     1081 tgcaaacctg gcctccactg gcgggattgc catttatgga tggttggtga cgattatcgg
     1141 tgcgctgggg ctctcgatgg tatacgccaa aatgtcgttc ctcgacccaa gtcctggtgg
     1201 ttcttacgct tacgcccgcc gctgctttgg cccgtttctc ggttatcaaa ccaacgtcct
     1261 ctactggctg gcctgctgga tcggcaatat cgccatggtg gtcattggcg taggatattt
     1321 aagttacttc ttcccgattc tgaaagaccc attggtatta accatcacct gcgtcgtggt
     1381 gctgtggatc ttcgtcctgc tgaacattgt cggtccgaaa atgatcaccc gcgtacaggc
     1441 cgttgctacc gtgctggcgc tgatccccat cgtcgggatt gccgtatttg gctggttctg
     1501 gttccgtggt gaaacctata tggcggcatg gaacgtcagc ggtctgggca ccttcggtgc
     1561 gattcaaagt acccttaacg ttacgctgtg gtcgttcatc ggtgtggaaa gtgcctccgt
     1621 tgccgcaggt gtggtgaaaa acccgaaacg caacgtccct atcgccacca ttggcggggt
     1681 attgattgct gccgtttgct atgtactttc taccaccgcg attatgggga tgatccctaa
     1741 tgccgcactg cgcgtttctg cttcgccatt cggtgatgcc gcacggatgg cgttgggtga
     1801 caccgccggg gccattgttt ccttctgcgc agctgcgggt tgcttaggtt cactgggcgg
     1861 ctggacgttg ctggcgggtc aaacggcgaa agccgctgcc gatgacggac tgttcccacc
     1921 gatttttgcc cgtgttaata aagcgggcac gccagtggcg gggctgatta tcgtcggtat
     1981 tttgatgacc atcttccagc tcagtagcat ttcgccaaac gcgaccaaag aattcggtct
     2041 ggtttcttcc gtgtcggtca tctttacact ggtgccatat ctttacacct gtgcggcgtt
     2101 actactgctc ggccacggtc actttggtaa agcacgcccg gcatacctgg cagtgactac
     2161 cattgccttc ctctactgca tctgggccgt ggtagggtcc ggcgcgaaag aggttatgtg
     2221 gtcatttgtc actctgatgg taatcaccgc catgtatgcg ctgaattaca accggctgca
     2281 taaaaacccg tatcccttag atgcaccaat aagcaaagat taattcccct taatcaagca
     2341 aacataaaag ccaaccttaa gaacttaagg ttggcttaat tttgctttgc gagcatatgc
     2401 gcactttgtt cgatggaaac accgtgatgt tgaagcgcct actaaaaaga ccctctttaa
     2461 atttattctc ctggttatta cttgctactt tttatatctc agtctgcctg aatatcgcct
     2521 tttttaaaca ggtgctacag gtgttaccgc tggattcact gcataacgtg ctggttttct
     2581 tgtcgatgcc ggtcgtcgca ttcagcgtga ttaatattgt cctgacacta ggctctttct
     2641 tatggcttaa tcgtccactc gcctgcctgt ttattctggt cggtgcggct gcacaatatt
     2701 tcatcatgac ctatggcatc gtcatcgacc gttcaatgat caccaatatc attgatacta
     2761 cgccggcgga aagttatgcc ctgatgacgc cacgaatgtt attaacgctg gggttaagtg
     2821 gcgtgttagc cgcgattatt gcctgctgga tcaaaatcac ccccaccacc tcacgcctgc
     2881 gcagcgttct tttccgcggg gccaacatcc tgatttctgt actgctgatt ctgctggttg
     2941 ccgcactgtt ctacaaagac tatgcctcgc tgtttcgcaa caacaaagag ctggtgaaat
     3001 ccttaagccc gtccaacagc attgttgcta gctggtcgtg gtactctcac cagcggtcag
     3061 ccaacctgcc actgatacgc attggtgaag atgctcaccg caacccatta atgcaaaatg
     3121 ggaaacgtaa aaacctgacc attttgatcg tcggagaaac gtcgcgcgca gagaatttct
     3181 cgctgaatgg ttatccacgc gaaaccaatc ctcgtctggc aaaagataac gtggtctatt
     3241 tccctaatac cgcctcttgc ggcacggcaa cggcagtttc agtaccatgc atgttctcgg
     3301 atatgccgcg tgagcactac aaagaagagc tggcacagca ccaggaaggc gtgctggata
     3361 tcattcagcg agcgggcatc aacgtgctgt ggaatgacaa cgatggcggc tgtaaaggcg
     3421 tttgcgatcg cgtacctcac cagaacgtca ccgcgctgaa tctgccaggt cagtgcatca
     3481 acggcgaatg ctatgacgaa gtgctgttcc acgggctgga agagtacatc aataacctgc
     3541 agggtgatgg cgtgattgtc ttacacacca tcggcagcca cggtccgacc tattacaacc
     3601 gctatccgcc gcagttcagg aaatttaccc caacctgtga cactaacgag atccagacct
     3661 gtaccaaaga gcaactggtg aacacttacg acaacacgct ggtttacgtc gactatattg
     3721 ttgataaagc gattaatctg ctgaaagaac atcaggataa atttaccacc agcctggttt
     3781 atctttctga ccacggtgag tcgttaggtg aaaatggcat ctatctgcac ggtctgcctt
     3841 atgccatcgc cccggatagc caaaaacagg tgccgatgct gctgtggctg tcggaggatt
     3901 atcaaaaacg gtatcaggtt gaccagaact gcctgcaaaa acaggcgcaa acgcaacact
     3961 attcacaaga taatttattc tccacgctat tgggattaac tggcgttgag acgaagtatt
     4021 accaggctgc ggatgatatt ctgcaaactt gcaggagagt gagtgaatga aaattctgat
     4081 tgttgaagac gatacgctgt tattgcaggg attaattctg gcggcgcaaa ccgaaggcta
     4141 cgcgtgcgat ggcgtgacaa ccgcgcggat ggcggaacaa agccttgagg ccggtcatta
     4201 cagcctggtg gtactggatt tagggttacc cgacgaagat ggactgcatt ttctcgcccg
     4261 tatccggcag aaaaaataca ccctgccggt actgatcctc accgctcgcg atacgctgac
     4321 cgacaaaatc gccgggctgg atgtcggtgc cgatgactat ctggtgaagc cttttgcgct
     4381 ggaagagtta cacgcccgta tccgcgccct gctacgacgc cataataatc agggcgaaag
     4441 tgagctgatt gttggcaatc tgacgctgaa tatgggtcgc cgtcaggtat ggatgggcgg
     4501 tgaagagttg attctgacgc ccaaagaata tgctctgctg tcacggttaa tgctcaaagc
     4561 aggcagtccg gtgcatcggg aaattctcta caacgacatc tataactggg acaatgaacc
     4621 ctcgaccaac accctggaag tgcatatcca caatctgcgc gacaaagtgg gcaaagcccg
     4681 tatccgcacc gtgcgcggct ttggctatat gctggtcgca aatgaggaaa actaattgaa
     4741 tctgatgcgt tttctgcgcc gaccaatatc gctgcgccaa cggctgatat tgaccattgg
     4801 ggccattttg ttggtgtttg agctgatcag cgtcttctgg ctatggcatg aaagtaccga
     4861 gcagattcag ctgtttgagc aggcgctacg cgacaatcgc aacaacgatc gccacatcat
     4921 gcgtgagatc cgcgaggcgg tcgccagcct gatcgtcccc ggcgtcttta tggtcagcct
     4981 gacgctattt atctgctatc aggcggtacg ccgcatcacc cggccgctgg cggagctgca
     5041 aaaagagctg gaagcgcgca ccgccgacaa cttaacgcct attgccattc acagcgccac
     5101 cctcgaaatc gaagcggtgg tttcggcgtt gaacgatctg gtcagtcgcc tgaccagcac
     5161 gctggataac gaaaggttgt ttaccgctga cgtcgcgcac gaactgcgaa cgccactggc
     5221 gggggtgcgt ttgcatctgg aactgctggc gaaaacgcat cacattgatg tagcaccgtt
     5281 agtggctcga cttgatcaga tgatggagag tgtctcccag ctgctgcaac tggcgcgtgc
     5341 cggacagtca ttttcttccg gtaattatca acatgtaaaa ctgctggaag atgtcattct
     5401 cccctcgtat gacgaactca gtaccatgct cgaccagcga cagcaaaacc tgctattgcc
     5461 agagagcgcc gccgacatca ccgttcaggg tgatgcaacc ctgctgcgga tgttattgcg
     5521 aaacctggta gaaaacgccc atcgttacag cccgcaaggc agcaacatta tgattaagct
     5581 gcaagaagat ggcggagcgg tcatggccgt tgaagatgaa ggaccgggta ttgatgagag
     5641 taaatgcggg gagttgagta aagcgtttgt acgtatggac agccgttatg gcgggattgg
     5701 tctggggtta agtattgtca gccgcattac acagttgcat cacgggcagt ttttcctgca
     5761 aaaccggcaa gagacttccg gcacgcgggc ctgggtacgg ctgaagaaag atcagaacgt
     5821 ggcaaaccag atataaagaa agctgctgac caccagcacg ctgaacacgg tagttaaact
     5881 ttcataaaca cggtttttca ttgcactctc ctccgttaac ctggaggaga gtatgcgcgt
     5941 cagagattaa gtgaacctta agagttcaat tggcctgata agacagcgtc acatcaggcc
     6001 atccgtttca gcttattcat caattcgcgg atgttgctgc accaggcgag tacgtttcgc
     6061 ctgcaaatcg gcaatctcgt ggtcaatatc atcgattttc tgctcgatat tatcgtaatg
     6121 ctcgacgaga atttccttcg cttcctgtat atctgacgcc gccggtgtcg cacctttcaa
     6181 cggacgattt gccgtctctt tcatggttac gccggtgatt aaaccaacca ccgccactac
     6241 catcaggtaa taggcaggca tcatcagatt ctgcgagctt tcgaccagcc aggccgccag
     6301 tgttggcgtc agaccggcaa ccagcaccga aatattaaat gccgccgcca gcgcgctgta
     6361 acggatatgc gtcggaaaca tcgctggcaa ggtagaagcc ataacgcccg taaagcagtt
     6421 aaggatcacc gccagcatca gtaacccggc aaaaatcagg ccgatgacgt tactgttaat
     6481 cagaataaac gccgggatcg ccaacacaaa cagtgcaaca ctaccaagta gcacaaacgg
     6541 acgacggcca aaacggtcac tcagcaagcc catcaccggc tggacaaaca gcataccgat
     6601 cataatggcg ataataatca gcaccccgtg gtcttcggaa taatgcaggt tatgcgacaa
     6661 ataactcggc atataggtca gcaacatgta gtaagtcacg ttggtggcaa ttaccagacc
     6721 aatacatgtc aacaggctgc gccagtgttt ggtggcaatc tctttaaacg agactttcgg
     6781 gccatcctgc aaaccttcgc ggtcgccctg ctccagttta tcaacatgct gctggaacgc
     6841 cggggtctct tccagcgcat ggcgcaggta aagcccgata atccctaacg gcagagcaat
     6901 aaagaacgga atacgccagc cccagtcgag gaagttcgct tcgccgacaa tggtcgaaat
     6961 taacaccacc acgcccgcac ccagcacaaa cccggcaata gaaccgaagt ccagccagct
     7021 gcccataaag ccacgtttac ggtcagggga gtattccgca acaaatatcg acgccccggt
     7081 atattcaccg ccgaccgaga aaccttgtgc catcttacag atcaacagca gaatcggtgc
     7141 ccaaatacca atcgtgtcgt aggacggtat taagccaata cagaacgtac tgatcgacat
     7201 aatcacaata gtgatagcga ggatcttctg gcgaccatat ttatcgccca acataccaaa
     7261 gaagagtcca ccaagcggtc gaatcagaaa gggaacggag aaagtggcaa gtgcagcaac
     7321 catctgcacg ctggggtcag cccccgggaa aaaaacttta cctaatgcgt aagcaacaaa
     7381 accataaaca ccaaaatcaa accattccat tgcattaccc agtgatgctg cggtaatggc
     7441 tttacgcagt ttaccgtcat caataatggt gacatcacga agggtaattg gttttacttt
     7501 tttccttttc agcatagctt tcctcgcaga gttggcccat ttccgcaaac gccagaaaaa
     7561 tcagcgcgac ggccgcaaaa ggggagcggg aattgcagga tgcaatctct gtaacaagcg
     7621 tagcgggttt gcttacactc tccggttaag gaatgtacaa tcaaaccagt tgacgcctga
     7681 attgggcagt taatgacctt tttaccctac cagcgtttaa atttgtgatc aacttcacac
     7741 atggatttcg tttaggactc attgatgtaa ctgattattt acaccaaatt tacagagaag
     7801 atttcctcga ccattgcccc cgccagacgc tgcgctgaac agagtcgtgg catccccagt
     7861 gcgacagttc gccagcgttg ggtcactgac cagcaaacgg gttgtttatc tgccgtggac
     7921 cactttccca gccaacttaa aaaatcagca tgatccccgc ccgtcggaat ggtcgaagag
     7981 gcaaaccact gatggtaata gtgacgcgtg gtcggggccg cattgatact ctctgccaga
     8041 tgttgtgtcg ctttcacgcg caaatcaccc agcaacaacg ccaccgcttg cgggcttccc
     8101 agacgaaagg catcgccaaa tgccccccaa cgcaactcct cccgaacgat aaaacagcgc
     8161 ccaggcaacg accaggcgtt gtacgcccca gcacgccagc gggtaaaaat ttgctcgctc
     8221 tgttcacccg cctgcaccag taggcctcgt tgcgttaacg ccttttcctt ccagcgacaa
     8281 cgggctgaaa accactcata caaggcggca atttgctggg ggagctcagg gaaagcagag
     8341 tgctggcggt gatccatttt ttgcagcagc gccagatgtt gcgcgatctg cgtcagcgcc
     8401 agatgaccgg gatccagcat cgacgccagt ttttccacca atgtcagacg caggctaagc
     8461 ggcgcattta acatcccgcc gttcgtccat gcctgaagct gcacacgcgc aagaagttcc
     8521 tgcatcagtc gttcacgaaa ctgctgctga tgggcttgtg gatggcgggc atcatcgccc
     8581 tgcgccagat ccactaaaaa gcggggatga atcgactcca gcacgcgacc ggggccgtcc
     8641 agtaacgtct tggtcaatat cgttctgccg tgaaaagtgt ttgaatatca tcgcgtaaca
     8701 gctgagcgtc ggtgtaaatc cagccatgag tcatcacagt ctgctgcaat tgctgctgca
     8761 tcagcctgac caccgattca ttttgttgac gcagagccag actttcgcgt aaacgcgtct
     8821 gtaattccgt caaacatgaa gcgaactcag cgaaaaaagt attcatgcct gccgtaacag
     8881 attcatcgac ctgctctgcc agaactttag ccatttgttg gcaataaaga tcgacttctg
     8941 cgcttaatgc tcgttgcaac gcactgtaat caaccgtctc tatcggggat ttctcatttc
     9001 cccgtcccca gtcgggctga ttcaaccagc gcgaaaaagt ctcacgcaca acgcctaaac
     9061 gcgtgctctg ctcgtccatt gcgtcctggc gcgaaatgac ttcactgaac agctggcgag
     9121 tgttgaagtg gggaactacg ccgtgaaaaa caggaaaatg aaacccagga cgaaaccctg
     9181 actcgctcaa ttccattttg atttgttgct caatggggcg aataacatcg gttaacaccc
     9241 gggcaaaggg tggattccag ctcggcaaaa cgcagcgtaa agtcgcgact aatggtgttc
     9301 tgcgccatct gtaacagtgt ctcacagcag gtacgaatct cgcttaatga ccctgaatca
     9361 tcctgaaaca aggcggctaa ctgcgcattc agcgcatctt gctgttgacg cagaaagtgg
     9421 ttggcggagg tcagggccag ctcgatttca tgtttaatct cgccgctcac ctgcgcctga
     9481 ttgagttgca atagctgcaa actttcttcg atctgatgga tattttgccg caattgttca
     9541 caagcgacgt ttaacccgtg cgcacgaaaa tccaggtatt cccgcgcctg ctgcgcgtaa
     9601 ttcaacagtt tatgcgcggc agatcgcaga gcatacaacg aggcgttagc gtaagcggca
     9661 tgaagcaacg cctgaattgg ctgggcgaac agcgaatctt cccacaactg atcggcagca
     9721 tgacgaatat gttcgaggtc cgccagatcg gcatgacgcc agcgcctgcc gagcgcggca
     9781 tgggcaaaat cttccaccca gcgttgttgc tctggcggtg gtaacttacc gttgttggct
     9841 aactcatggc gcacccgatt cgccaggtag ccccacatcg acgacaccgg aaatatctgc
     9901 tgtggcgtaa tacaaccttt catcagcgtc ccggaaatca gtgcccgcac ctggtcggcg
     9961 tcgtcactgt tacgatcctg ttgatcgaac ttattgacca gcacatacaa cggcaccgat
    10021 tgccccaccg ccaaaattgc ctcacggacc tcttcatcgg agatcgattt cagttgcgta
    10081 taatccagca ccgccagtac cgccgaggcg cgtgccagct gctggttaag cattttttgc
    10141 agatgcggtt gcccggcttc atttggcccg ggggtatcca gtaacgtcaa ctgaccggga
    10201 taactctcca gccccgccag atggacaaac tccacttcaa tcacgggaat atgctcaatg
    10261 gcggcgtaag cagaaaaagg aaaatcgacg tccagcgcct tcgccagtcg caccaaatca
    10321 ttcaaacttt tcagacaatg aaaaataggt tgagcaccca gataatattt ttcgaaagcg
    10381 atgccatttt caatccgctg cataagcgca cgcatatctt tatctatttc cagcacatcg
    10441 gtcagatgct taatatcgca atcacgcagg cgctgttgta attgttgaat taaatggtcg
    10501 attggcgcga catgtgaaaa atgcagtacc ggttcttttt gcccgggcgt atggcgaata
    10561 agcgttggca gcgcagtcat tgggcgatta cgattaggca gaacctccgt accaacaatg
    10621 gcattaatgg tggttgattt ccctgctttc atggtaccga caatcgccag caccatttcc
    10681 agccgggaaa ttttacgcaa ctcattattc agcatcgcgt gacgttcggc gatattaggc
    10741 tgactccagg gtaaagccag ttgtggcgta tcgtctccgg gtacagagag aggtattttt
    10801 tccagtaact gcaactgttg gcgagaaagc tgtaacaggc gttcagcctc ctgacttaac
    10861 tcatacaggg tctgtgtata catagaatat tcttccttaa agcaaatttt attactttta
    10921 tttagccaga ttgtttttga gttctgtttt cggcttttat aattactgca ggaaataatt
    10981 ttatatttag cgtgttgttt tttattagaa caaataacat cttccaatac gtttaaaacg
    11041 tcagaaagat aaaaacatca tgtgaattaa aaaaagaacg agtagagcat taaccttatc
    11101 ttaaataata aatggaggca aaaagattat tttctttttg cgtttccttt caaatgaaaa
    11161 cgatcgtcgt ctaaaatcag cagtaccccc gacaaactca gggattttgt gtataattgc
    11221 ggcctttttc ggcaatctgc cgttttttgg cgcttttgcc ctgctgactt ttgaggaaat
    11281 ccacatgtca ttaccacact gcccaaaatg caactccgaa tacacctacg aagataacgg
    11341 catgtacatc tgcccggaat gtgcctacga atggaacgac gcagaacctg cacaggaaag
    11401 cgacgagctg atcgttaaag atgctaacgg caatctgctg gctgacggcg acagcgttac
    11461 catcattaaa gatctgaaag tgaaaggtag ctcttcgatg ctgaaaattg gcaccaaagt
    11521 gaaaaacatc cgcctggttg aaggcgacca taacatcgat tgcaaaatcg acggttttgg
    11581 tccgatgaaa ctgaagtctg agtttgtgaa aaagaactga ttgtattgtg atcggtaggc
    11641 cggataaggc gtttacgccg catccggcaa cggtggcgac tgcctgatgc gacgctgacc
    11701 gcgtcttatc aggcttccct ctcatctgta taaatttcga actacactta acgggcttct
    11761 cttaactgag gtcaccatca tgccgttaag tccctacctc tcttttgccg gtaactgttc
    11821 cgacgcgatt gcctattatc aacgtacgtt gggcgcggaa ctgctctata aaatcagctt
    11881 cggcgaaatg ccgaaatcag cgcaggacag cgccgagaac tgcccttccg gaatgcaatt
    11941 tcccgatacc gccatcgctc atgccaacgt gcgcattgca ggaagcgaca tcatgatgag
    12001 cgatgctata ccgtcaggaa aagccagcta ctccggcttt acgctagtgc tcgattcgca
    12061 acaggtcgaa gaaggaaaac gctggtttga taaccttgca gctaacggaa aaatcgaaat
    12121 ggcctggcag gaaactttct gggcgcatgg cttcggcaaa gtcaccgata aatttggcgt
    12181 accgtggatg attaatgtcg tcaaacaaca accaacgcac taacccgccg ggagggccgc
    12241 cctcccgcac tgtcatcgaa atctcgttaa ctcttcatcc gttagtcact tttagttaac
    12301 aaaatcgtca caataatccg ccacgatgga gccacttttt tagggaggct gcatcatgca
    12361 aacgattatc cgtgtcgaga agctcgccaa aaccttcaat cagcatcagg cgctgcacgc
    12421 ggttgatctg aacattcatc acggtgaaat ggtggctctg cttgggccgt cgggttccgg
    12481 caaatccacc cttttacgtc acttaagcgg tttgattacc ggcgataaat ctgccggtag
    12541 ccatatcgag ctgctgggcc gcacagtcca gcgcgaaggc cgcctggccc gcgatatccg
    12601 caaaagccgc gccaataccg gctacatctt ccaacaattc aacctggtga atcgcctgag
    12661 cgtactggag aacgtgctga ttggcgcgct cggcagcacg ccattctggc gcacctgttt
    12721 tagctacttc acccgcgagc agaaacagcg cgcgttacag gcgctgaccc gcgttggcat
    12781 ggtgcacttt gcccatcagc gcgtttccac cctctccggc ggacagcaac agcgtgtggc
    12841 gattgcccgc gcgctgatgc agcaggcgaa ggtgattctg gcggacgaac ccatcgcctc
    12901 gctggacccg gaatcggcgc gcatcgtgat ggacaccctg cgcgacatta accagaacga
    12961 cggcattacc gtggtcgtca cgctgcatca ggtggattac gccctgcgct actgcgaacg
    13021 catcgtcgcc ctgcgccagg ggcacgtctt ctacgacggc agcagccaac agtttgataa
    13081 cgaacgtttt gaccatctct accgcagcat caaccgcgtc gaagaaaacg cgaaagctgc
    13141 ctgacatccc catcattgag gaaaacgaat gaacgctaag ataattgcct cgctgacctt
    13201 caccagcatg ttcagcctca gcaccctgtt aagcccggcg cacgccgaag agcaggaaaa
    13261 ggcgctgaat ttcggcatta tttcaacgga atcacagcaa aacctgaaac cgcaatggac
    13321 gccgttctta caggatatgg agaagaagct gggcgtgaag gtcaacgcct tctttgcccc
    13381 ggactacgcg ggcattatcc agggaatgcg cttcaacaaa gtggatatcg cctggtacgg
    13441 caatctgtca gcgatggaag cggtggatcg cgccaatggc caggtcttcg cccagacggt
    13501 cgcggcggat ggatcgccgg gttactggag cgtgttgatc gtcaacaaag atagtccgat
    13561 caacaacctg aacgatctgc tggccaagcg taaagatctc accttcggca atggcgatcc
    13621 taactccacc tcaggcttcc tcgtccccgg ctactacgtc ttcgccaaaa acaatatctc
    13681 cgccagcgac ttcaagcgca ccgtcaacgc cgggcatgaa accaacgcgc tggccgttgc
    13741 caacaagcag gtggatgttg ccaccaacaa caccgaaaac ctcgacaagc tgaaaacctc
    13801 cgcaccggag aagctcaaag aactgaaggt gatctggaaa tcgccgctga tcccaggcga
    13861 tccgatcgtc tggcgcaaaa atctgtccga aaccaccaaa gacaagatct acgacttctt
    13921 tatgaactac ggcaaaacgc cggaagagaa agcggtgctg gaacgcctgg gctgggcccc
    13981 gttccgcgcc tccagcgacc tgcaactggt gccgattcgc cagctcgcgc tgtttaaaga
    14041 gatgcagggc gtgaaaggca ataaagggct gaatgagcag gacaagctgg caaaaaccag
    14101 cgccattcag gcgcaactgg atgacctgga ccgcctgaac aacgcgttaa gcgcgatgag
    14161 ttcggtgagt aaagcggtgc agtaaatcgt aggtcggata agacgccccg gcgtcgcatc
    14221 cgacaatgtg cagacgttga tgccggatgc ggtgcaagca ccttatccgg cctacagacc
    14281 ggagccaaac atgcaaacca tcaccatcgc cccacccaag cgcagctggt tctcgcttct
    14341 gagctgggcc attgttctcg ccgtgttggt cgtctcgtgg cagggcgcgg aaatggcccc
    14401 gcttacgctg atcaaagacg gcggcaacat ggcaaccttc gccgccgact tcttcccgcc
    14461 cgatttcagc cagtggcagg attacctcac cgaaatggcc gtcacgctgc aaatcgccgt
    14521 ctggggcacc gcgctggcgg tggttctctc catccccttc ggtctgatga gcgccgaaaa
    14581 cctggtgccg tggtgggttt accagcccgt acgccgcctg atggacgcct gccgcgccat
    14641 taacgaaatg gtcttcgcca tgctgttcgt ggtcgccgtc ggccttggcc cgttcgctgg
    14701 cgtgctggcg ctgtttatcc acaccaccgg cgtgctctcc aagctgcttt ccgaagcggt
    14761 agaagcgatc gaacccggcc cggtggaggg cattcgcgcc accggtgcca acaagctgga
    14821 agagatcctc tacggcgtgc tgccacaggt gatgccgctg ctgatctcct actccctcta
    14881 tcgcttcgaa tccaacgtcc gctcggcgac cgtcgtcggc atggtcggtg caggcgggat
    14941 cggcgtcacc ctgtgggaag cgattcgcgg tttccagttc cagcaaacct gcgccctgat
    15001 ggtgctcatc atcgtcacgg tcagcctgct ggatttcctc tctcaacggt tgcgtaagca
    15061 ctttatctga taagcgaggc attgatagct atgcacttgt ctacacatcc gaccagctac
    15121 ccaacacgct atcaggagat agccgcaaaa cttgagcagg agcttcgtca acactaccgc
    15181 tgcggcgact atcttcccgc cgagcagcaa ctggcagcgc gctttgaggt gaaccgccac
    15241 accctgcgcc gcgccatcga ccaactggtg gaaaaaggtt gggtacagcg ccgtcagggc
    15301 gtcggcgtgc tggtgctgat gcgcccgttc gattacccgc tcaacgccca ggcgcgtttt
    15361 agccagaacc tgctggacca aggcagccat cccaccagcg aaaaattgct atcggtgctg
    15421 cgccccgcct ccggtcacgt cgctgacgcg ctggggataa ccgaggggga gaacgtcatc
    15481 cacctgcgca ccctgcgccg ggtcaacggc gtcgcgctct gtttaatcga ccactacttt
    15541 gcggacctca ccctctggcc gacgctgcaa catttcgaca gcggttcgct gcacgatttt
    15601 ctgcgcgagc aaacggggat tacgctacgc cgcagtcaga cgcggatcag cgcccgccgc
    15661 gcccaggcca aagagtgcca gcgtcttgaa atcccgaatc tatcgccgct gctgtgcgtg
    15721 cgcaccctta accaccgtga cggtgaaagc agcccggcgg agtactccgt cagcctgacg
    15781 cgcgccgaca tgattgaatt cactatggag cactgaatgc acgcagatac cgcgacccgc
    15841 cagcactgga tgtccgtact ggcgcacagc caaccgactg agctggcagc ccgcctgaac
    15901 gcgctgaaca tcaccgccga ctatgaagta atccgcgccg ctgaaaccgg cctggtgcag
    15961 attcaggcgc ggatgggtgg taccggcgaa cgcttttttg ctggcgacgc cacgctcacc
    16021 cgcgccgccg tacgcctgac tgacggcacg ctcggctaca gctgggtgct ggggcgcgac
    16081 aaacagcacg ccgaacgctg cgcgctgatt gacgcgctga tgcagcaaac ccgccatttt
    16141 caaaacttat cagaaactct tattgccccg ctgaacgctg accgtatggc acgcattgcc
    16201 gcacgccagg ccgaagtaaa cgccagccgg gtcgacttct ttacgatggt tcgcggagat
    16261 aacgcatgac cctggaaacc gcttttatgc ttcccgtgca ggatgcccag cacagttttc
    16321 gtcgcctgtt aaaagccatg agcgagccgg gcgtgattgt cgccctgcat cagctcaaac
    16381 gcggctggca gccgctgaat atcgccacca ccagcgtgct gctgacgctg gccgataacg
    16441 acacgccggt gtggctttct gcgccattaa gtaacgatat cgtcagccag agcctgcgtt
    16501 ttcataccaa cgcgccgctg gtcaatcagc cggaactggc gaccttcgcg gtaacggatg
    16561 aggcgatttc cagcgaacag cttaacgccc tttccagcgg caccgctgtt gcgccagaag
    16621 cgggcgcgac gctgatttta caggtcgtca gcctgagcgg cgggcgcatg ctgcgcctta
    16681 ctggcgcggg tattgccgaa gaacgaatga tcgccccgca gctgccggag tgcattctgc
    16741 acgaacttac cgagcgcccg cacccattcc cgctcggcat cgacctgatc ctgacctgcg
    16801 gcgagcgcct gctggctatc ccgcgaacca ctcatgtgga ggtatgctga tgtacgttgc
    16861 cgtgaaaggg ggcgagaagg cgatcgacgc cgcccacgcc ctgcaagaga gccgacgccg
    16921 gggcaatacc gatttgcctg aactgagcgt cgcccagatt gaacagcagc ttaatcttgc
    16981 agtagatcgc gtgatgaccg aaggcggcat tgccgaccgc gaactggcgg cgctggcgct
    17041 gaaacaggcc agcggcgata acgtcgaagc gattttcctg ctacgcgcct accgcaccac
    17101 gctggcgaag ctcgcagtaa gcgagccgct cgacaccacg gagatgcgtc tcgaacggcg
    17161 tatttcagcg gtttataaag atattcccgg cggccagctg cttggcccaa cctacgacta
    17221 cacccatcgc ctgctcgatt tcaccctgct ggcaaacggc gaagcgccga cgctgaccac
    17281 agccgacagc gaacaacagc cgtcgccgca cgttttcagc ctgctggcgc gtcaggggct
    17341 ggcgaagttt gaagaagata gcggcgcaca gccggacgac atcacccgca cgccgccggt
    17401 ttacccctgc tcacgctcct cccgcctgca acaattaatg cgcggcgacg aaggctattt
    17461 gctggcgctg gcctactcca cccagcgtgg ttacggacgc aatcatccgt tcgcaggcga
    17521 aatccgcagc ggctatatcg acgtgtcgat tgtgcaggaa gagctgggat ttgcggtgaa
    17581 cgtcggcgaa ctgctgatga ccgaatgtga aatggtcaac ggttttatcg acccgccgga
    17641 tgagccgccg cacttcacgc gcggctacgg gctggtgttc ggcatgagcg agcgcaaagc
    17701 gatggcgatg gcgctggttg accgcgctct acaagccccg gagtacggcg agcacgcgac
    17761 aggcccggcg caggatgaag agttcgtgct ggcgcatgcc gacaacgtcg aagccgcagg
    17821 ctttgtctcg caccttaaac tcccgcacta cgtcgatttc caggccgaac tggagctact
    17881 caaacgtctg caacaggagc agaaccatgg ctaatctgag cggctacaac tttgcctacc
    17941 tcgacgagca gaccaaacgc atgatccgcc gcgccatctt aaaagcggtg gcgatccccg
    18001 gttatcaggt gccgtttggc gggcgcgaga tgccgatgcc gtacggctgg ggaaccggcg
    18061 gcatacagct taccgccagc gtgattggcg aaagcgatgt gctgaaggtg attgaccagg
    18121 gcgcggacga caccaccaac gcggtgtcga tccgtaactt ctttaagcgc gtgaccgggg
    18181 taaacaccac tgaacgtacg gacgatgcga cgcttatcca gacgcgccac cgcatccccg
    18241 aaacgccgct gaccgaagat cagatcatta ttttccaggt gccgatcccc gaaccgctgc
    18301 gctttatcga gccgcgcgaa acggaaaccc gcaccatgca cgcgctggag gagtacggcg
    18361 tgatgcaggt gaagctgtat gaagatatcg cccgcttcgg tcatatcgcc accacctacg
    18421 cctatccggt gaaggtaaac gggcgctacg taatggaccc gtcgccgatc ccgaaatttg
    18481 ataacccgaa aatgaacatg atgcccgccc tgcaactgtt cggcgcgggg cgcgagaagc
    18541 gcatctatgc ggtgccgccg tttacccgcg tggaaagcct cgatttcgac gatcacccgt
    18601 tcaccgttca gcagtggaat gagccatgcg ccatctgcgg atcgacccac agctatcttg
    18661 atgaagtggt gctggatgac gccggaaacc gcatgtttgt ctgctccgat accgattatt
    18721 gccgccaaca gagcgaggcg aaaagccaat gaatcaaccg ttactttcgg tcaataacct
    18781 gacccacctt tacgcgccgg gcaaaggctt tagcgatgtc tcttttgatt tatggccggg
    18841 ggaagtgctg ggcatcgtcg gggaatccgg ctccgggaag accacgctgc tgaagtcgat
    18901 ctccgcgcgc ctgacgccgc agcaagggga aattcgctat gaaaaccgtt ccctctatgg
    18961 catgagcgag gccgaccgcc gtcgcctgct gcgcaccgaa tggggcgtgg tgcatcagca
    19021 tccactcgac ggcctgcgcc gccaggtgtc ggcaggcggc aatatcggcg agcggctgat
    19081 ggcgaccggg gcgcgtcatt acggcgatat tcgtgccacc gcgcagaagt ggctggaaga
    19141 ggtggaaatt cccgccaacc ggatcgacga cctgccgacc accttttccg gcggtatgca
    19201 gcagcgtttg cagattgccc gcaacctggt gacgcatccg aagctggtat ttatggacga
    19261 gccaaccggc gggctggatg tatctgtgca ggcgcgcctg ctcgacctgc tgcgcggcct
    19321 ggtggtggag ctgaacctgg cggtggtgat tgtcacccac gatttaggcg tcgcccgcct
    19381 gctggcggac cgtttgctgg tgatgaagca ggggcaagtg gtggagagtg ggttaaccga
    19441 ccgcgtgctc gacgacccgc accatccgta tacacaactg ctggtgtcat cggttttgca
    19501 gaattgagcc ggtgccggat gcggcgtaaa cgccttatcc ggcctacaaa tgcgctcccc
    19561 gtaggtcgga taagacgcgt cagcgtcgca tccgacaccc gaaccacgag gcgaaaaatg
    19621 attaacgtac aaaacgtcag taaaaccttc atcctgcatc agcaaaacgg cgtgcgcctg
    19681 cccgtcttac aacgtgcctc gctcaccgtc aacgcaggcg aatgcgtggt gcttcacggc
    19741 cactccggca gcggaaaatc aaccctgctg cgctcgctgt acgccaacta tctgcccgac
    19801 gaagggcaaa tccagatcaa acacggtgag gaatgggtag acctggtcac cgcgccagcc
    19861 cgcaaagtgg tggagatccg caaaaccacc atcggctggg tgagccagtt tctgcgcgtc
    19921 atcccgcgta tttcggcact ggaagtggtg atgcagcctc tgctcgatac cggcgtaccg
    19981 cgtgaagcct gcgccgctaa agccgcccga ttactgacgc gcctgaacgt gcctgaacgc
    20041 ctgtggcacc tggcaccatc gaccttctcc ggcggcgaac agcagcgcgt caacatcgcc
    20101 cgtggcttta tcgtcgacta tccgattctg ctgctggatg aacccaccgc ctcgctggac
    20161 gctaaaaaca gcgccgcggt ggtggaatta attcgcgaag ccaaagcccg tggcgcggcc
    20221 atcgtcggta tcttccatga cgaagccgta cgcaatgacg tcgccgaccg cctgcaccca
    20281 atgggagtct cttcatgatt atcaataacg tcaaactggt gctggaaaac gaagtggtgc
    20341 acggttcact ggaagtacag gatggcaaaa ttcgcgcctt tgccgaaagc cagagccgcc
    20401 agccggaagc gatggacggc gaaggtggct ggctgctgcc ggggctgatt gagctgcata
    20461 ccgataatct ggataaattc ttcaccccgc gtccaaaagt ggactggccc gcccactcgg
    20521 caatgagtag ccacgacgcg ctgatggtgg cgagcggcat caccaccgtg ctggacgcgg
    20581 tggcgattgg cgacgtgcgc gacggcggcg atcggctgga gaatctggag aagatgatca
    20641 acgccatcga agagacgcag aaacgcggcg tcaaccgcgc cgagcaccgt ctgcatctgc
    20701 gctgcgaact gccgcatcac accacgctgc cgctgtttga aaaactggtg cagcgcgaac
    20761 cggtgacgct ggtgtcgctg atggatcact cgccgggcca gcgccagttc gctaaccgcg
    20821 agaagtatcg cgaatattat cagggcaaat actcgctcac cgaagcgcag atgcagcagt
    20881 atgaagaaga gcagctggcg ctcgccgcac gctggtcgca gccaaatcgc gaaaccatcg
    20941 ccgcaatgtg ccgcgcgcga cacattgcgc tcgccagcca cgatgacgcc acgcacgccc
    21001 acgttgctga atctcaccaa cttggcagcg taatcgccga atttcccacc acgttcgaag
    21061 cggcagaagc ctcgcgcaag catggcatga acgtgctgat gggcgcaccg aatatcgtgc
    21121 gcggcggttc gcactccggc aacgtggcgg ccagtgaact ggcgcagctt ggcctgctgg
    21181 atatcctctc ttccgactac taccccgcca gcctgctcga tgcggcattt cgcgtcgccg
    21241 atgacgagag caaccgcttt acgctgccgc aggcggtgaa gctggtgact aaaaatccgg
    21301 cgcaggcgct taatcttcag gatcgcgggg tgattggcga gggcaaacgc gccgacctgg
    21361 tgctggcgca tcgcaagggc aatcacattc atatcgacca cgtctggcgt cagggtaaaa
    21421 gggtgttctg atgacgggaa aactgatttg gttaatgggg gcctctggct ccggaaaaga
    21481 cagtctgttg acggaactcc gccagcggga acaaactcag ctactggtag cgcatcgcta
    21541 catcacgcgc gccgccagcg ccggaagtga aaaccatatc gccctgagcg agcaggagtt
    21601 ttttacccgc gcggggcaga accttctggc cttaagctgg cacgctaacg gtctgtatta
    21661 tggcgtcggc gtcgagattg acctctggct gcacgctgga tttgacgtgg tggtcaacgg
    21721 ctcacgcgcc catctgccgc aggcgcgggc gcgctatcaa tcggcgctgc tgcccgtttg
    21781 tttacaggtt tcgccagaga tcctgcgcca gcgcctggaa aaccgtggtc gtgaaaatgc
    21841 cagtgaaatt aacgcccggc tggcgcgcgc tgcccgctat actcctcagg attgtcttac
    21901 gctcaataat gacggcagcc tgcgccagtc ggtcgacaag ttgctgacgc tgattcatca
    21961 gaaggagaaa caccatgcct gcttgtgagc ttcgcccggc tacgcagtac gacaccgacg
    22021 cagtttacgc gctgatttgt gagctaaaac aggcggagtt tgaccaccag gcgtttcgcg
    22081 tggggtttaa cgccaatctg cgcgatccga acatgcgcta ccaactggcg ctgctggatg
    22141 gcgaagttgt cggcatgatc ggcctgcatt tgcagtttca tctgcatcat gtcaactgga
    22201 tcggcgaaat tcaggaactg gtggtgatgc cgcaggcgcg cggtctgaat gtcggcagca
    22261 aattactggc atgggcagaa gaagaagccc gccaggccgg ggccgaaatg actgagcttt
    22321 ccaccaacgt gaagcgccac gacgcgcacc gattttacct gcgcgaaggc tacgagcaga
    22381 gccacttccg cttcaccaag gtgctgtaac atgagcctga ccctcacact tactggcact
    22441 ggcggcgcac agggcgttcc cgcatggggc tgtgagtgcg tggcctgcgt cagagcgcgg
    22501 cgctcaccgc agtatcgccg ccagccatgc agcggcgtcg tgaagtttaa cgacgcgatc
    22561 accctgatcg acgccgggcg gcacgatctc gccgatcgct ggtcgcccgg atcgttccag
    22621 cagtttttgc tgacgcacta ccatatggat cacgtgcagg ggctgtttcc gctgcgctgg
    22681 ggcgtgggcg atcagatccc ggtctacggc ccgccggatg aacagggctg cgacgatcta
    22741 ttcaaacatt cgggcctgct tgatttcagc cacacggtgg agccatttgt gatgtttgat
    22801 ttgcagggat tacaggtcac gccgctaccg ctcaaccact caaaactcac tttcggctat
    22861 ttactggaaa cggcgcacag ccgggtggcg tggctatctg acaccgcagg cctgccggaa
    22921 aaaacgctga aatttttacg caataatcag ccgcaggtaa tggtgattga ttgcagccac
    22981 ccgccgcgcg aggatgcacc gcgtaatcac tgtgatttaa ataccgtgct tgcgctgaat
    23041 caggttatcc gctcgccgcg ggtgattctg acccatatca gccatcagtt tgatgcgtgg
    23101 ctgatggaaa acgcgctacc atcggggttt gaggcggggt ttgatgggat ggagattgag
    23161 gtggcgtgag ggaaaatacc cgcgccggcc cctatatcaa cgcccatact catcttccat
    23221 tctccgctct tcatcttcca gttgccgacg ctcctgatca agctggcgct ggcgatcgtc
    23281 cagctgcctg cggcgatctt caaactggcg gcggcggtca tcatattgtc tgcgccgatc
    23341 gtcgctcact tcacgctgcc agccgtcgtc gcgcgaatct tcatagtctc gcccacggtc
    23401 agggttataa gcgtcgttaa tcgcctgctg aatattgcca atggtgtcgt cgataatatc
    23461 ggcctgggcc ggaacggtgg acagcgtgag cagggtgaat aaaagaaata gcggaaagcg
    23521 tttcattagc caacctcaaa aagaaactct atccacatta gtcattactc atccatgcaa
    23581 gtagtggacg aatctcaatt tctccgctgc tctattgccg taatcgcctc cacgcgttgt
    23641 tgatgacgac cgccttcgta ctgtgcgccc agccacgcat cgacaatcat ttttgccagt
    23701 tcgaggccaa ccactcgtga accaaaagcc agcacgttgg tgtcgttatg ctgccgcgaa
    23761 agttgcgcgg aataaggttc gctacaaacg accgcgcgaa ttccggcaaa cttgttcgcc
    23821 gctatcgaaa taccgacgcc agtgccacaa atcaaaatcc cgccatcagc ctctccgcca
    23881 gcaacggcca gtgcgacgtg actggcgtaa tgcggataat cagtacgctc tgacgaccag
    23941 gttcctttat caatcacttc aacgccacgc tcaactaaat gtgccactat ttcatgtttt
    24001 aaaatgaaac cgacatgatc acagccaaat gcaatctttt tcattcatct tccccgcccg
    24061 cgcaaaactt cacaatcaaa aaacatttgt gatgtaaatg aattaaaaac acccaaaatc
    24121 gatgaaatcc gccttcccga tctgttttac agagttacat ctggcagcgc aatagcgtta
    24181 aagacactca ctatccagca acatctatca tctaat
//
LOCUS       DAZFLD010000060        24172 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.060, whole genome shotgun sequence.
ACCESSION   DAZFLD010000060 DAZFLD010000000
VERSION     DAZFLD010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24172)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24172)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24172
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.060"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            129..413
                     /gene="pduA"
                     /locus_tag="ACHKBL_002011"
     CDS             129..413
                     /gene="pduA"
                     /locus_tag="ACHKBL_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduA"
                     /protein_id="HFO5818888.1"
                     /translation="MQQEALGMVETKGLTAAIEAADAMVKSANVLLVGYEKIGSGLVT
                     VIVRGDVGAVKAATDAGAAAARNVGEVKAVHVIPRPHTDVEKILPKGINP"
     gene            410..1222
                     /gene="pduB"
                     /locus_tag="ACHKBL_002012"
     CDS             410..1222
                     /gene="pduB"
                     /locus_tag="ACHKBL_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduB"
                     /protein_id="HFO5818889.1"
                     /translation="MSSNELVEQIMAQVIARVATPEHEVLPKTTHQIRETAMAEKSCT
                     LTEFVGTAIGDTVGLVIANVDSALLDAMKLEKRYRSIGILGARIGAGPHIMAADEAVK
                     ATNTEVISIELPRDTKGGAGHGSLILLGGNDVSDVKRGIEVALKELDRTFGDVYANEA
                     GHLELQYTARASYALEKAFGAPLGRACGVIVGAPAAVGVLMADTALKSANVDVVAYSS
                     PAQGTSYSNEVILIISGDSGAVRQAVISAREIGKTVLSTLGDTPKNDRPSYI"
     gene            1241..2905
                     /gene="pduC"
                     /locus_tag="ACHKBL_002013"
     CDS             1241..2905
                     /gene="pduC"
                     /locus_tag="ACHKBL_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol dehydratase large subunit PduC"
                     /protein_id="HFO5818890.1"
                     /translation="MRSKRFEALAKRPVNQDGFVKEWIEEGFIAMESPNDPKPSIKIV
                     NGVVTELDGKPANQFDLIDHFIARYGINLARAEEVIAMDSVKLANMLCDPNVKRSDIV
                     PLTTAMTPAKIVEVVSQMNVVEMMMAMQKMRARRTPSQQAHVTNIKDNPVQIAADAAE
                     GAWRGFDEQETTVAVARYAPFNAIALLVGSQVGRPGVLTQCSLEEATELKLGMLGHTC
                     YAETISVYGTEPVFTDGDDTPWSKGFLASSYASRGLKMRFTSGSGSEVQMGYAEGKSM
                     LYLEARCIYITKAAGVQGLQNGSVSCIGVPSAVPSGIRAVLAENLICSSLDLECASSN
                     DQTFTHSDMRRTARFLMQFLPGTDFISSGFSAVPNYDNMFAGSNEDAEDFDDYNVIQR
                     DLKVDGGLRPVREEDVIAIRNKAARALQAVFAGMGLPPITDEEVEAATYAHGSKDMPE
                     RNIVEDIKFAQEIINKNRNSLEVVKALAQGGFTDVAQDMLNMQKAKLTGDYLHTSAII
                     VDDGQVLSAVNDVNDYAGPATGYRLQGERWEEIKNIPGALDPNEID"
     gene            2915..3583
                     /locus_tag="ACHKBL_002014"
     CDS             2915..3583
                     /locus_tag="ACHKBL_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004016448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol/glycerol family dehydratase medium
                     subunit"
                     /protein_id="HFO5818891.1"
                     /translation="MEINEKLLRQIIEDVLAEMQTSDKPVSFRAPVSATASSAPDTGN
                     FLTEIGEAQQGTQQDEVIIAVGPAFGLAQTVNIIGIPHKNILREVIAGIEEEGIKARV
                     IRCFKSSDVAFVAVEGDRLSGSGIAIGIQSKGTTVIHQQGLPPLSNLELFPQAPLLTL
                     DTYRQIGKNAARYAKRESPQPVPTLNDQMARPKYQAKSAILHIKETKYVVTGKKPQEL
                     RVTF"
     gene            3598..4116
                     /gene="pduE"
                     /locus_tag="ACHKBL_002015"
     CDS             3598..4116
                     /gene="pduE"
                     /locus_tag="ACHKBL_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol dehydratase small subunit PduE"
                     /protein_id="HFO5818892.1"
                     /translation="MNTDAIESMVRDVLNRMNSLQDAAPVSAVPNASIQSAKVTDYPL
                     ANKHPEWVKTATNKTLDDFTLENVLSDNVTAQDMRITPETLRIQAAIARDAGRDRLAM
                     NFERAAELTSVPDDRILEIYNALRPYRSTKQELIAIADDLERRYQAKICAAFVREAAE
                     LYVERKKLKGDD"
     gene            4126..5958
                     /locus_tag="ACHKBL_002016"
     CDS             4126..5958
                     /locus_tag="ACHKBL_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diol dehydratase reactivase subunit alpha"
                     /protein_id="HFO5818893.1"
                     /translation="MRYIAGIDIGNSSTEVALATLSATGELSYVSSALAETTGIKGTL
                     RNVHGIQEALAQATKKVGINVSDISLIRINEATPVIGDVAMETITETIITESTMIGHN
                     PKTPGGVGLGVGLTITPQELLTRPADTPYILVVSSAFDFADIATMINASVRAGYQLTG
                     VILQRDDGVLVNNRLEIPLPIVDEVLYIDRIPLGMLAAIEVAVPGKVIETLSNPYGIA
                     TVFALNAEETKNIVPVARALIGNRSAVVVKTPSGDVKARSIPAGNIELLSAGRTTRVD
                     VAAGADAIMKAVGECPKLENVTGEPGSNIGGMLEHVRQTMAELTNKPSNEIFIQDLLA
                     IDTSVPVNVTGGLAGEFSLEQAVGIASMVKSDRLQMAMIASEIKQKLHVDVQVGGAEA
                     EAAIQGALTTPGTTRPLAILDLGAGSTDASIINQKGEIVATHLAGAGDMVTMIIAREL
                     GLNDRYLAEEIKKYPLAKVESLFHLRHEDGSVQFFPTPLSPHVFARVCVVKPDELVPI
                     LGDLTLEKVRAVRRSAKERVFVTNALRALRQVSPAGNIRDIPFVVLVGGSSLDFEVPQ
                     LVTDALAHYRLVAGRGNIRGSEGPRNAVATGLLIAWHKGAIHGK"
     gene            5948..6298
                     /locus_tag="ACHKBL_002017"
     CDS             5948..6298
                     /locus_tag="ACHKBL_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000377973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol dehydratase reactivase beta/small
                     subunit family protein"
                     /protein_id="HFO5818894.1"
                     /translation="MESKERAPAIVVMEIGDCITTWDEVLLGIEEEGIPFRIQHIPSG
                     EVIDSAWLAARQSPLLVGIACDQEKLIVHYKNLPASAPLFTLMYQQDNHARRSIGTNA
                     ARLVKGIPFRELHS"
     gene            6318..6593
                     /gene="pduJ"
                     /locus_tag="ACHKBL_002018"
     CDS             6318..6593
                     /gene="pduJ"
                     /locus_tag="ACHKBL_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduJ"
                     /protein_id="HFO5818895.1"
                     /translation="MNNALGLVETKGLVGAIEAADAMVKSANVQLVGYEKIGSGLITV
                     MVRGDVGAVKAAVDAGSAAASAVGEVKSCHVIPRPHSDVEAILPKSA"
     gene            6617..7039
                     /locus_tag="ACHKBL_002019"
     CDS             6617..7039
                     /locus_tag="ACHKBL_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001607636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HFO5818896.1"
                     /translation="MKQSLGLLEVSGLALAISCADAMAKAASITLVGLEKTNGSGWTL
                     IKITGDVASVNAAIATGASFAGQRNGLVAHKVIARPGDGILPHTTEALTVTVETSETP
                     ATTVISEIISCNLCLDPACQRQKGEPRSLCLYANKRGE"
     gene            7042..7674
                     /locus_tag="ACHKBL_002020"
     CDS             7042..7674
                     /locus_tag="ACHKBL_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate propanoyltransferase"
                     /protein_id="HFO5818897.1"
                     /translation="MDKQTLQETVSQILDEMRERPIPLGVSSRHIHLSTADYLRLFPE
                     QPISEKKPLMQPGQFAAEQVVTLEGPKGKLHNVRLLGPLRNASQVEISRTDARTLGIT
                     APLRMSGDLSGTPGIRLISPFAELEITSGVIVAQRHIHMSPLDALIYGVTHGDKVAVA
                     IEGDERRLIFENVTVRVSPDMRLEMHIDTDEANAAGADSPDVFATLVSVT"
     gene            7671..8162
                     /gene="pduM"
                     /locus_tag="ACHKBL_002021"
     CDS             7671..8162
                     /gene="pduM"
                     /locus_tag="ACHKBL_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcompartment protein PduM"
                     /protein_id="HFO5818898.1"
                     /translation="MNNMLMQRIVDEVVFRLKQRAGKTLVLTVFQLRDASVQESVHQY
                     ASLQIRYVDLPLLRQLAENETSDRAAIQIHEALAWGMHIQLSLQRHFLNAIELKTLAR
                     LPLSWCDEQGQPIYLHHDRLLSYVDIAQLSSGILVLQRKCCVTALAREAAITRNIQLI
                     RQE"
     gene            8166..8441
                     /locus_tag="ACHKBL_002022"
     CDS             8166..8441
                     /locus_tag="ACHKBL_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HFO5818899.1"
                     /translation="MHLARVTGAVVSTQKSPSLIGKKLLLVRQVSADGELPLTPLTGD
                     EVAVDSVGAGVGELVLLSSGSSARHVFSGPNEAIDLAVVGIVDTLSR"
     gene            8453..9460
                     /gene="pduO"
                     /locus_tag="ACHKBL_002023"
     CDS             8453..9460
                     /gene="pduO"
                     /locus_tag="ACHKBL_002023"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-domain cob(I)yrinic acid a,c-diamide
                     adenosyltransferase PduO"
                     /protein_id="HFO5818900.1"
                     /translation="MAIYTRTGDSGSTSLFTGQRVSKTHLRVETYGTLDELNATLSLC
                     YCATAIESHRILLEAIQQQIFWFSAELASESEQPSAQQRYIGTEEIAALENAIDSAMN
                     AVPPVHCFILPGRCEAASRMHFARTVARRAERRLVELTTETTVRNVLLHYINRLSDCL
                     YALARVEDNVAHQNLMIQEITKRYHEANHTPALKERTMPLTFQDLHQLIRSAAMRADE
                     LHIPVVISIVDANGTESVTWRMPDALLVSSELAPKKAWTAVAMKTATHKLADTVQPGA
                     PLYGLESHMQGKVVTFGGGFPLWRDGKLLGGLGISGGSVEQDMDIAQSAMAAINVGVN
                     Q"
     gene            9457..10842
                     /gene="pduP"
                     /locus_tag="ACHKBL_002024"
     CDS             9457..10842
                     /gene="pduP"
                     /locus_tag="ACHKBL_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating propionaldehyde dehydrogenase
                     PduP"
                     /protein_id="HFO5818901.1"
                     /translation="MNNSELETLIRNILREQLVPATQRAQRNAIFQTVDEAVCAAHQA
                     FLRFQQCPLKTRSAIISAIREELMPRLTELAEESAKETGMGNKEDKYLKNKAALDNTP
                     GIEDLTTTALTGDGGMVLFEYSPFGVIGSVTPSTNPTETIINNSISMLAAGNSVYFSP
                     HPGAKNVSLKLITIIEDIAFRCSGIHNLIVTVSEPTFAATEQMMAHPKISVLAITGGP
                     GIVAMGMKSGKKVIGAGAGNPPCIVDETADIVKAAEDIINGASFDYNLPCIAEKSLIV
                     VVSVAEYLIQQMQSFGALLLNHEQTEKLRAICLPDGSANKKLVGKSPSALLEAAGLPL
                     PAKAPRLLIAVVDADDSWVTCEQLMPMLPIVKVNDFDSALALALKVEDGLHHTAIMHS
                     QNVSRLNLAARVMQTSIFVKNGPSWAGIGVGGEGFTTFTIATPTGEGTTSARTFARSR
                     RCVLTSGFSIR"
     gene            10853..11965
                     /locus_tag="ACHKBL_002025"
     CDS             10853..11965
                     /locus_tag="ACHKBL_002025"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0051144 - propanediol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-propanol dehydrogenase PduQ"
                     /protein_id="HFO5818902.1"
                     /translation="MKTFSLQTRLYSGTGSLNILNRFTNRHIWIICDVFLAHSPLIDT
                     LHQALPDSNRLSIFSEITPDPTIQTVVKGIAQMQTLRPDVVIGFGGGSALDAAKAIVW
                     FGRQCGIEIETCVAIPTTSGTGSEVTSACVISDPEKGIKYPLFDNALYPDIAILDPSL
                     VVSVPPAITANTGMDVLTHALEAYVSPRASDFTDALVEKAVQIVFQYLPTAVKKGDCL
                     ATRGKMHNASTLAGIAFSQAGLGLNHAIAHQLGGQFHLPHGLANALLLTAVIHFNAGD
                     PRAAKRYARLAKTCHLCPDNANDTASLNALIQHIEQLKTTCTLPTLTNALKEKKAEWS
                     IRIPDMVQAALADATLRTNPRAADASAIAELLEELL"
     gene            11962..13305
                     /locus_tag="ACHKBL_002026"
     CDS             11962..13305
                     /locus_tag="ACHKBL_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFO5818903.1"
                     /translation="MNVELTSCDDQTIRERVQAAGVVGAGGAGFPTHIKLQARVDTFL
                     VNAAECEPMLKVDQQLMVQQASRLLRGVHYAMKATGASSGIIALKEKYQRAINALTPL
                     LPPDIRLHILPDVYPAGDEVLTIWMATGRRVPPAALPVNVGVVVNNVQTVLNIARAVE
                     QQYPVTRRTLTINGAVAKPITVTVPIGMSLREVLALAGGATVDAPGFINGGPMMGNLI
                     TSLDTPVSKTTGGLLVLPNSHSLIQRRMQNDRSVLTVAKTVCEQCRLCTDLCPRHLVG
                     HELAPHLLVRAVNYQQLATPQLLLTALTCSECNVCASVACPVGISPMRINRLLKQELR
                     AQNLRYEGALNPADPMANYRLIPVKRLVTRLGLIPWYQDAPLSEQVPQPEKVTLLLRQ
                     HIGASAIACVQKGDRVVHGQCVGQIPHGTLGAPIHASIDGMVSDVTENAITLVRG"
     gene            13308..13862
                     /gene="pduT"
                     /locus_tag="ACHKBL_002027"
     CDS             13308..13862
                     /gene="pduT"
                     /locus_tag="ACHKBL_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduT"
                     /protein_id="HFO5818904.1"
                     /translation="MSKAVGILELSSIAKGMETGDAMLKSANVELLVSKTLCPGKFLL
                     MLGGDVGAVQQAINSGASLAGEMLVDSLVLPNIHPSLLSAISGLNHVEQHRAVGVVET
                     WSVAACIDAADCAVKAANVILVRVHMAFGIGGKCYMVVAGDISDVNNAVSVASERAGE
                     KGLLVYRSVIARPHEAMWRQMVEG"
     gene            13862..14212
                     /gene="pduU"
                     /locus_tag="ACHKBL_002028"
     CDS             13862..14212
                     /gene="pduU"
                     /locus_tag="ACHKBL_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000441103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduU"
                     /protein_id="HFO5818905.1"
                     /translation="MTTQHTTERMIQEYVPGKQVTLAHVIANPGKDLFKKLGLPDTVS
                     AIGILTITPSEASIIACDIATKSGAVEIGFLDRFTGAVVLTGDVSAIEYALSQVTRTL
                     GEMMRFTACPITRT"
     gene            14217..14660
                     /locus_tag="ACHKBL_002029"
     CDS             14217..14660
                     /locus_tag="ACHKBL_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutP/PduV family microcompartment system
                     protein"
                     /protein_id="HFO5818906.1"
                     /translation="MQRMMLIGPSQCGKTSLTQCLRGENIHYQKTQAIVWSPTTIDTP
                     GEYLENRSLYSALLVSACEADIIALVLNADAPWSPFSPGFTSSMNRPVIGVVTKADLA
                     SMEQISLVKCWLREAGAHNVLVTSAVNNNGVTELFALLHTEEGCR"
     gene            complement(14761..15090)
                     /gene="tmaR"
                     /locus_tag="ACHKBL_002030"
     CDS             complement(14761..15090)
                     /gene="tmaR"
                     /locus_tag="ACHKBL_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF496 family protein"
                     /protein_id="HFO5818907.1"
                     /translation="METTKPSFQDVLEFVRLFRRKNKLQREIQDVEKKIRDNQKRVLL
                     LDNLSDYIKPGMSVEAIQGIIASMKGDYEDRVDDYIIKNAELSKERRDISKKLKAMGE
                     MKNGEAK"
     gene            complement(15262..16320)
                     /gene="yeeA"
                     /locus_tag="ACHKBL_002031"
     CDS             complement(15262..16320)
                     /gene="yeeA"
                     /locus_tag="ACHKBL_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFO5818908.1"
                     /translation="MRADKSLSPFEIRVYRHYRIVHGTRVALAFLLTFLIIRLFTIPE
                     STWPLVTMVVIMGPISFWGNVVPRAFERIGGTVLGSILGLIALQLELISLPLMLVWCA
                     AAMFLCGWLALGKKPYQGLLIGVTLAIVVGSPTGEIDTALWRSGDVILGSLLAMLFTG
                     IWPQRAFIHWRIQLAKSLTEYNRVYQSAFSPNLLERPRLESHLQKLLTDAVKMRGLIA
                     PASKETRIPKSIYEGIQTINRNLVCMLELQINAYWATRPSHFVLLNAQKLRDTQHMMQ
                     QILLSLVHALYEGNPQPVFANTEKLNDAVEELRQLLDNHHDLKVVETPIYGYVWLNME
                     TAHQLELLSNLICRALRK"
     gene            complement(16518..16991)
                     /gene="sbmC"
                     /locus_tag="ACHKBL_002032"
     CDS             complement(16518..16991)
                     /gene="sbmC"
                     /locus_tag="ACHKBL_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor SbmC"
                     /protein_id="HFO5818909.1"
                     /translation="MNYEIKQEEKRTVAGFHLVGPWEQTVKKGFEQLMMWIDSKNIVP
                     KEWVAVYYDNPDETPAEKLRCDTVVTVPNNFTLPENSEGVILTEISGGQYAVAVARVV
                     GDDFAKPWYQFFNSLLQDSAYEMLPKPCFEVYLNNGAEDGYWDIEMYVAVQPKHH"
     gene            complement(17110..18276)
                     /gene="dacD"
                     /locus_tag="ACHKBL_002033"
     CDS             complement(17110..18276)
                     /gene="dacD"
                     /locus_tag="ACHKBL_002033"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase DacD"
                     /protein_id="HFO5818910.1"
                     /translation="MKRRLIIAASLFVFNLSSGFAAENIPFSPQPPEIHAGSWVLMDY
                     TTGQILTAGNEHQQRNPASLTKLMTGYVVDRAIDSHRITPDDIVTVGRDAWAKDNPVF
                     VGSSLMFLKEGDRVSVRDLSRGLIVDSGNDACVALADYIAGGQRQFVAMMNNYAEKLH
                     LKDTHFETVHGLDAPGQHSSAYDLAVLSRAIIHGEPEFYHMYSEKSLTWNGITQQNRN
                     GLLWDKTMNVDGLKTGHTSGAGFNLIASAVDGQRRLIAVVMGADSAKGREEEARKLLR
                     WGQQNFTTVQILHRGKKVGTERIWYGDKENIDLGTEQEFWMVLPKAEIPHIKAKYTLD
                     GKELTAPISAHQRVGEIELYDRDKQVAHWPLVTLESVGEGGMFSRLSDYFHHKA"
     gene            18485..19912
                     /gene="sbcB"
                     /locus_tag="ACHKBL_002034"
     CDS             18485..19912
                     /gene="sbcB"
                     /locus_tag="ACHKBL_002034"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008310 - single-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HFO5818911.1"
                     /translation="MMNDGKQQSTFLFHDYETFGTHPALDRPAQFAAIRTDSKFNVIG
                     EPEVFYCKPADDYLPQPGAVLITGITPQEARAKGENEAAFAARIHSLFTVPKTCILGY
                     NNVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDG
                     LPSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLM
                     ALIDVPQMKPLVHVSGMFGAWRGNTSWVAPLAWHPENRNAVIMVDLAGDISPLLELDS
                     DTLRERLYTAKADLGDNAAVPVKLVHINKCPVLAQANTLRPEDADRLGINRQHCLDNL
                     KILRENPQVREKVVAIFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLP
                     ALDITFVDKRIEKLLFNYRARNFPGTLDYAEQQRWLEHRRQVFTPEFLQGYADELQML
                     AQQYADDKEKVALLKALWQYAEEIV"
     gene            complement(19986..21344)
                     /gene="plaP"
                     /locus_tag="ACHKBL_002035"
     CDS             complement(19986..21344)
                     /gene="plaP"
                     /locus_tag="ACHKBL_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PlaP"
                     /protein_id="HFO5818912.1"
                     /translation="MSHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVS
                     GLTDGHVPTAYAFALIAILFTALSYGKLVRRYPSAGSAYTYAQKSISPTVGFMVGWSS
                     LLDYLFAPMINILLAKIYFEALVPSIPSWMFVVALVAFMTAFNLRSLKSVANFNTVIV
                     VLQVVLIAVILGMVVYGVFEGEGAGTLASTRPFWSGDAHVIPMITGATILCFSFTGFD
                     GISNLSEETKDAERVIPRAIFLTALIGGMIFIFATYFLQLYFPDISRFKDPDASQPEI
                     MLYVAGKAFQVGALIFSTITVLASGMAAHAGVARLMYVMGRDGVFPKSFFGYVHPKWR
                     TPAMNIILVGAIALLAINFDLVMATALINFGALVAFTFVNLSVISQFWIREKRNKTLK
                     DHFQYLFLPMCGALTVGALWVNLEESSMVLGLIWAAIGLIYLACVTKSFRNPVPQYED
                     VA"
     gene            complement(21334..21396)
                     /gene="yoeI"
                     /locus_tag="ACHKBL_002036"
     CDS             complement(21334..21396)
                     /gene="yoeI"
                     /locus_tag="ACHKBL_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YoeI"
                     /protein_id="HFO5818913.1"
                     /translation="MGQFFAYATVITVKENDHVA"
     gene            complement(21611..22540)
                     /gene="yeeY"
                     /locus_tag="ACHKBL_002037"
     CDS             complement(21611..22540)
                     /gene="yeeY"
                     /locus_tag="ACHKBL_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFO5818914.1"
                     /translation="MKPLLDVLMILDALEKEGSFAAASAKLYKTPSALSYTVHKLESD
                     LNIQLLDRSGHRAKFTRTGKMLLEKGREVLHTVRELEKQAIKLHEGWENELVIGVDDT
                     FPFSLLAPLIEAFYQHHSVTRLKFINGVLGGSWDALTQGRADIIVGAMHEPPSSSEFG
                     FSRLGDLEQVFAVAPHHPLAQEEEPLNRRIIKRYRAIVVGDTAQAGASTASQLLDEQE
                     AITVFDFKTKLELQISGLGCGYLPRYLAQRFLDSGALIEKKVVAQTLFEPVWIGWNEQ
                     TAGLASGWWRDEILANSAIAGVYAKSDDGKSAI"
     gene            complement(22586..23410)
                     /gene="yeeZ"
                     /locus_tag="ACHKBL_002038"
     CDS             complement(22586..23410)
                     /gene="yeeZ"
                     /locus_tag="ACHKBL_002038"
                     /EC_number="1.1.1.290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFO5818915.1"
                     /translation="MKKVAIVGLGWLGMPLAMSLSARGWQVTGSKTTQDGVEAARMSG
                     IDSYLLRMEPELVCDSDDLDALMDADALVITLPARRSGPGDEFYLQAVQELVDSALAH
                     RIPRIIFTSSTSVYGDAQGTVKETTPRNPVTNSGRVLEELEDWLHNLPGTSVDILRLA
                     GLVGPGRHPGRFFAGKTAPDGEHGVNLVHLEDVIGAITLLLQAPKGGHIYNICAPAHP
                     ARNVFYPQMARLLGLEPPQFRNSLDSGKGKIIDGSRICNELGFEYQYPDPLVMPLE"
     gene            complement(23493..23747)
                     /gene="yoeB"
                     /locus_tag="ACHKBL_002039"
     CDS             complement(23493..23747)
                     /gene="yoeB"
                     /locus_tag="ACHKBL_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YoeB"
                     /protein_id="HFO5818916.1"
                     /translation="MKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGK
                     PEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAACRYHY"
     gene            complement(23744..23995)
                     /gene="yefM"
                     /locus_tag="ACHKBL_002040"
     CDS             complement(23744..23995)
                     /gene="yefM"
                     /locus_tag="ACHKBL_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoeB-YefM toxin-antitoxin system antitoxin YefM"
                     /protein_id="HFO5818917.1"
                     /translation="MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEE
                     YNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKDIIE"
BASE COUNT         6202 a         6264 c         5997 g         5709 t
ORIGIN      
        1 agttcataaa agcaatttta ttatatttgt tctctattaa tttttctcat tagccgatta
       61 agtcagccat tctttttcct cgcttttttt tatagtcata tcgtcaaaaa aatccaggcg
      121 aggtctttat gcaacaagaa gcgttaggaa tggtagaaac caaaggctta actgccgcca
      181 tagaggccgc agacgccatg gtgaagtcgg caaatgtttt gctggtcggc tacgaaaaaa
      241 taggttccgg gctggtgacc gtcatcgttc gtggtgatgt cggggcggta aaagcggcaa
      301 cagacgcggg tgccgcagct gcgcgtaacg tcggcgaagt aaaagccgta cacgttattc
      361 cccgcccaca taccgatgtc gaaaaaatct taccaaaggg aattaaccca tgagcagcaa
      421 tgaactggtc gaacagatca tggcgcaggt catcgctcgc gtagcgacgc cagagcatga
      481 ggttctcccg aaaaccactc atcaaatacg agagacggct atggcagaaa aaagctgcac
      541 gttaacagaa tttgttggca ccgctattgg cgacactgta ggtctggtca ttgcgaacgt
      601 tgatagcgcc ctgttggacg cgatgaaact tgaaaaacgc tatcgttcca ttggcattct
      661 gggggcgcgt attggcgcag ggccacacat tatggccgct gatgaagcgg taaaagccac
      721 caacactgaa gttattagca ttgaacttcc tcgggatact aaaggcggtg ccggacacgg
      781 ctcattgatc ctcttaggtg gcaacgatgt ttccgacgtg aaacgcggta ttgaagttgc
      841 cctgaaagaa ctcgatcgga cctttggtga tgtctatgcc aacgaagccg ggcatcttga
      901 actgcaatac accgcccgcg ccagctatgc actggaaaaa gcgtttggcg ctccactggg
      961 acgggcttgc ggcgttattg tcggcgctcc ggctgcggtg ggtgttctga tggccgacac
     1021 cgcgctgaag tccgccaatg tggatgtcgt ggcatacagc tcccccgctc aaggcaccag
     1081 ctacagcaac gaagtcattc tgattatctc gggtgactcc ggcgcagtcc gccaggcggt
     1141 catctccgca cgcgaaatcg gtaaaaccgt gctttcgact ctcggcgata caccgaaaaa
     1201 cgatcgccct tcctacatct gaattctctg aggcagattt atgagatcga aaagatttga
     1261 agcactggca aaacgcccgg tcaaccagga cggtttcgtt aaagagtgga ttgaagaagg
     1321 cttcattgcg atggaaagcc cgaacgatcc taaaccgtca atcaaaattg ttaacggcgt
     1381 cgttaccgaa ctggatggaa aacccgccaa tcagtttgac ctgatcgacc actttatcgc
     1441 ccgctacggc atcaatctcg cgcgcgcaga agaagtcatc gcgatggatt cggtaaaact
     1501 tgccaatatg ttgtgcgatc caaatgtcaa acgtagcgat atcgttcccc ttaccacagc
     1561 aatgacgcct gcaaaaatcg tcgaagtggt ttcgcagatg aacgtggtag agatgatgat
     1621 ggcgatgcag aaaatgcgcg cccgtcgaac accatcacaa caggctcacg tcaccaacat
     1681 caaagataac ccggtacaaa ttgccgccga cgcagctgaa ggcgcatggc gcgggtttga
     1741 tgaacaggaa accaccgttg ccgttgctcg ctatgcaccg ttcaacgcca tcgccctgct
     1801 ggtcggctca caggtgggac gtccgggcgt cttgacccag tgttcactgg aagaagccac
     1861 cgaactgaaa ctcggtatgt taggccacac ctgttacgcc gaaacaattt ctgtctatgg
     1921 tacagaacct gttttcaccg atggcgatga taccccgtgg tcaaaagggt ttctcgcgtc
     1981 atcctatgcg tctcgcggct taaaaatgcg ctttacttcc ggctccggct ctgaagtaca
     2041 gatgggttac gcggaaggca aatcaatgct ttatctcgag gctcgatgca tttacatcac
     2101 caaagccgca ggcgttcagg gtctgcagaa tggttcggta agctgtatcg gtgttccatc
     2161 agctgtgcca tcaggcatcc gcgcagtatt agcggaaaac ttaatttgct catcgctgga
     2221 tctggaatgc gcctccagta atgaccagac ctttacccac tcggatatgc gccgtaccgc
     2281 acgtttcctg atgcaatttc tgccaggtac cgactttatc tcttccggtt tttccgctgt
     2341 gccgaactac gacaacatgt ttgctggttc aaacgaagat gctgaagact tcgatgatta
     2401 caacgtcatt cagcgtgacc tgaaggttga tggcggcctg cgaccagtac gcgaagaaga
     2461 tgttatcgct atccgcaata aagccgctcg cgctttacag gctgtctttg ccggtatggg
     2521 actaccgcct attaccgatg aagaagtcga agcggcaacc tatgcccacg gttcaaaaga
     2581 tatgcctgag cgcaatattg tagaagacat caagttcgcc caggagatta tcaataaaaa
     2641 ccggaacagc ctggaagtgg tgaaagccct ggcgcaaggc ggttttaccg atgtcgccca
     2701 ggacatgctc aacatgcaaa aagcaaaact gaccggcgat tatctccata cctcagccat
     2761 catcgtcgat gacggacaag tgctctctgc ggtcaatgac gtcaatgatt acgccggacc
     2821 ggctacaggt taccgcctgc aaggggaacg ctgggaagag atcaaaaata ttcccggtgc
     2881 acttgatccc aacgaaattg actaagggtg agaaatggaa attaacgaaa aattactgcg
     2941 tcagattatc gaagacgtgc tggcagaaat gcaaaccagc gacaagcccg tttcatttcg
     3001 cgcacctgtc tctgcaactg cctcttccgc gccagatacc ggaaattttc tgaccgaaat
     3061 tggcgaagcc caacaaggca ctcagcaaga tgaagtcatt attgctgtag gcccggcgtt
     3121 cggcctggcg caaaccgtca atatcatcgg tattccgcat aaaaacattt tgcgcgaagt
     3181 gattgccggt atcgaagaag aaggcattaa agcccgtgtg attcgctgct ttaaatcgtc
     3241 cgatgtcgca ttcgtagcag tcgaaggcga tcgtcttagt ggttcaggta ttgctatcgg
     3301 tattcagtcg aaaggcacaa cggttatcca ccagcaggga cttcctccct tgtctaacct
     3361 ggaacttttc ccacaagcgc ctttgttgac gctggatact tatcgtcaga ttggcaaaaa
     3421 cgccgcgcgt tatgcaaagc gtgagtctcc acaaccggtg ccgaccctca acgatcaaat
     3481 ggcgcggccg aaataccagg ccaaatctgc cattttgcat attaaagaga cgaagtacgt
     3541 ggtcaccggc aaaaaaccgc aagaactgcg cgtgacgttt taacaaagga aaccttcatg
     3601 aataccgatg caattgagtc gatggtcaga gacgtgttga accgcatgaa cagtttacag
     3661 gatgcagctc ctgtttctgc ggtccccaac gcgtcaatac agagcgccaa agtgacggat
     3721 tacccgctgg caaataaaca tccggaatgg gttaaaacgg cgaccaataa aacgctcgac
     3781 gatttcactc tggaaaatgt gctaagcgat aacgtcaccg cccaggatat gcgtataacc
     3841 cctgaaacat tgcgtattca ggccgcaatt gccagagatg ctggtcgcga tcgcctggcg
     3901 atgaattttg aacgcgccgc tgagcttacc tcagtaccgg atgatcgcat cctcgaaatc
     3961 tacaatgcgt tacgccctta tcgttcgact aagcaagagc ttattgccat tgctgacgat
     4021 cttgaacggc gttatcaggc caaaatttgt gctgcatttg tccgtgaagc tgcggaactt
     4081 tatgtcgagc gcaaaaaact gaaaggcgac gattaaggac gaactatgcg atatatagca
     4141 ggcattgata tcggcaactc atcaacagag gttgcgctgg caacgctcag tgcaactgga
     4201 gagttgtcgt acgtgagtag cgccctggcg gaaaccaccg gaattaaagg tacgttgcgt
     4261 aacgtccatg gtattcagga ggcgctcgca caggcaacga aaaaagtcgg catcaatgtt
     4321 agcgatattt cgcttattcg tatcaacgaa gcaactccgg tcattggtga tgtggcgatg
     4381 gaaaccatca ctgaaaccat catcaccgaa tcaaccatga tcggtcacaa cccgaaaact
     4441 ccgggcggag ttgggctggg cgtagggctc accatcactc cgcaagagtt attaacacgc
     4501 ccggcggaca caccttacat cctggtggtg tcatcggcat ttgattttgc cgatatcgca
     4561 acgatgataa acgcctccgt acgagccgga tatcagctga ctggcgttat tctgcaacgc
     4621 gacgatggcg tactggtcaa taaccgcctg gaaattcctc tccctattgt ggatgaagtc
     4681 ctctacatcg atcgtattcc gctcggcatg ctggctgcaa ttgaagttgc cgttcccggg
     4741 aaagttatcg aaaccctgtc taacccgtat gggattgcca ccgttttcgc cctcaatgct
     4801 gaagagacta aaaatattgt tcctgtcgcg cgggcgctaa tcggcaaccg ttcagcagtg
     4861 gtagtgaaaa cgccttctgg cgacgttaaa gcccgttcga tacccgccgg aaatatcgaa
     4921 ctgctttctg ccgggcgcac aacgcgggtt gatgtagctg ctggtgccga tgccattatg
     4981 aaagcggtcg gtgagtgccc aaaactggaa aacgtgacgg gagaacctgg ctccaacatc
     5041 ggcggaatgc tggaacatgt tcgccagacc atggctgaac tgaccaacaa acccagcaac
     5101 gaaattttta ttcaggattt gctggcgata gatacctctg tccccgtcaa cgtaaccggt
     5161 ggtctggcag gtgaattttc actggaacag gctgtgggta ttgcctctat ggtgaaatcc
     5221 gaccgtctgc aaatggcgat gatcgccagt gaaattaagc aaaaactgca tgttgatgtt
     5281 caggtcggag gcgcagaagc agaagccgcc attcaggggg ccttgactac ccctggaacc
     5341 acacgtccac tggcaattct ggacttaggt gcaggctcta ccgatgcctc catcatcaat
     5401 cagaaagggg aaatagttgc cactcacctt gcgggagcgg gcgacatggt aacgatgatt
     5461 atcgctcgtg agctgggctt aaatgatcgt tatctcgcag aagaaatcaa aaagtacccg
     5521 ttggcaaaag tagaaagcct gttccactta cgccatgaag atggcagcgt tcagttcttc
     5581 cctactccgc tgtcccctca tgtatttgcc cgcgtttgtg tggtgaaacc tgacgaactg
     5641 gtgcctattc tcggtgattt gacgctggaa aaagtacgtg ctgtccgtcg tagtgcgaaa
     5701 gagcgtgtct tcgtaactaa tgccctgcgc gcgctacgtc aggtaagccc tgccggaaat
     5761 attcgcgaca ttccgttcgt cgttctggtg ggtggttctt cgctggattt cgaagtaccg
     5821 caattggtca ccgacgcgct ggcacattat cgactggtcg ccgggcgggg gaatatccgt
     5881 ggtagcgaag gtccgcgcaa tgcggtagct actgggctcc tcatcgcctg gcacaagggg
     5941 gcgattcatg gaaagtaaag agcgtgcacc agccatcgtt gtcatggaga tcggtgactg
     6001 tatcactaca tgggatgaag tgcttcttgg cattgaagag gaaggtattc cctttcgcat
     6061 tcaacacatt ccgtcaggtg aagttatcga tagcgcctgg ctggcggctc gtcaatctcc
     6121 cctgttggtt ggcattgcct gcgatcagga aaaactgatt gtgcattaca aaaacttacc
     6181 ggcatcagcg ccactgttta cgctgatgta tcaacaagat aaccatgcca ggcgcagcat
     6241 cggcactaac gctgcccgcc tggtaaaagg gattcccttc cgggaactcc actcataatc
     6301 cacaggagaa acgcagtatg aacaacgcac taggactggt tgaaacaaaa gggttagttg
     6361 gcgcaattga agctgcagat gccatggtta aatccgccaa cgtgcagctg gttggctatg
     6421 aaaagattgg ttcgggtctg attacggtga tggtccgtgg cgatgttggc gcagtaaaag
     6481 ccgccgttga tgcaggaagc gccgccgcaa gcgccgttgg cgaagtgaaa tcctgccatg
     6541 tcatcccgcg cccacacagt gatgttgagg ctattttacc taaatcagca taaccccatt
     6601 cgctaaggag cacagcgtga agcaatcact gggattactt gaagtcagtg gtctggcact
     6661 cgccatcagt tgcgcagatg ctatggcaaa agccgcgtcc atcacgctgg ttggccttga
     6721 gaagaccaat ggctcaggct ggacgctcat caaaatcacc ggtgacgtcg cctcggttaa
     6781 tgcggcgatc gcgacgggag ccagtttcgc cgggcagcga aatggccttg tcgcacacaa
     6841 agtaattgcc cgcccgggag acggcattct gccccacaca actgaagcat tgactgtgac
     6901 ggttgaaaca agcgaaacac cagcaacaac cgtcatctca gagattatca gttgtaattt
     6961 gtgtcttgac ccggcatgtc agcggcaaaa aggtgagccc cgctcacttt gcctgtacgc
     7021 caataagcga ggggagtagc agtggataaa caaaccctgc aggaaacagt gagccagatt
     7081 ctggatgaga tgcgcgagcg ccctatccca ctgggcgttt ccagtcgcca tattcatctt
     7141 tcaacagcgg attatttgcg tctttttcct gaacaaccga ttagcgagaa aaagccgtta
     7201 atgcaaccgg gccaattcgc tgctgaacag gttgttacgc tggagggacc aaaaggcaaa
     7261 ttgcacaacg ttcgcttgtt ggggccgcta cgcaatgcaa gccaggtgga aatttcccgg
     7321 acggacgcca gaacgttagg gatcaccgcc ccgctacgca tgtcaggcga tctcagcggc
     7381 actccgggga ttcgtttgat aagcccattt gcggaactgg agatcacatc tggcgtcatt
     7441 gtcgcgcaac ggcatattca tatgtctccg ctggatgccc tgatttatgg tgttacgcac
     7501 ggcgataaag tggcggttgc cattgaaggt gatgagcgac gactgatatt cgaaaacgtc
     7561 actgttcgcg tctctccgga tatgcgtctt gaaatgcata ttgataccga tgaagccaac
     7621 gccgcagggg ctgatagccc tgacgttttt gccacactgg tgtcagtgac atgaataaca
     7681 tgctgatgca acgtattgtt gacgaagtgg tttttcgcct taaacaacgt gcgggaaaaa
     7741 cactcgtgct gactgttttt caactgcgag acgccagcgt tcaagagagc gttcatcagt
     7801 atgcgtcatt acaaatccga tatgtcgatc tgccgctgct gcgacaactt gcggagaacg
     7861 aaacgtcaga tcgagcagca atccagatac acgaagcgtt agcctgggga atgcacattc
     7921 aattgtcgtt gcagcgtcat ttcctgaacg ccattgaatt gaagactctc gcccgtctac
     7981 cgctaagctg gtgcgacgaa caagggcaac cgatctacct acatcatgat cgacttttaa
     8041 gttatgtcga tatcgcacag ttaagctcgg gaatattggt gttacagcgg aaatgttgtg
     8101 tgaccgcact ggctcgtgaa gcggcaatta cccgaaatat tcagttaatc aggcaggagt
     8161 aacctatgca tctggctcga gtcactggcg cggtagtatc cacgcaaaaa tcaccttctc
     8221 tgatcgggaa aaaactgctg ctggttcgcc aggtcagtgc tgacggcgaa ctgcctctca
     8281 cgccattaac cggcgacgaa gttgccgttg attccgtcgg cgcaggcgtt ggtgagttgg
     8341 tactcctcag cagtggttcc agcgccagac atgtgttttc cggccctaac gaagccatcg
     8401 acctggcagt ggtcggcatt gtcgatacgc tttcgcgcta aggacctgaa agatggcaat
     8461 ttatacccga acaggtgatt caggaagtac gtctttattt accggacaac gcgtcagcaa
     8521 aactcatcta cgtgtcgaaa cctatggcac gctggatgaa ctgaacgcga cactcagtct
     8581 gtgttattgc gccaccgcca tcgaaagcca tcgcatctta ctggaagcca ttcaacaaca
     8641 gatattttgg tttagcgccg aactcgccag tgaaagcgaa cagccgtcag cgcagcagcg
     8701 ttacatcggt acggaagaaa ttgccgcgct ggaaaacgcg atcgatagcg caatgaacgc
     8761 cgtccccccg gttcactgct tcattctgcc tggtcgatgt gaagccgcaa gccgcatgca
     8821 tttcgcccgc acggtggctc gacgggctga aagacgtctg gttgaactga caacagaaac
     8881 caccgtccgg aatgttttgc tgcactatat caaccgactt tccgattgcc tgtatgccct
     8941 cgcccgcgtg gaagacaacg ttgctcatca aaacctgatg attcaggaaa tcacaaaacg
     9001 ttatcacgaa gccaaccaca caccagcatt gaaggaacgc acaatgccgc tcacttttca
     9061 ggatcttcac cagctcattc gtagtgcggc aatgcgcgca gatgaactgc atattcccgt
     9121 tgtcatcagc attgtcgatg ccaacggaac agaaagtgtt acctggcgga tgcctgatgc
     9181 cctgttggtc agcagtgaac tggcaccgaa aaaagcctgg accgccgtgg cgatgaaaac
     9241 ggcaacccac aagcttgctg ataccgtgca acccggcgcg ccgctttatg ggctcgaaag
     9301 ccatatgcaa ggcaaagtcg tcacctttgg tggcggcttc cccctctggc gtgacggaaa
     9361 attgcttggc gggcttggca tcagcggcgg tagcgttgaa caagacatgg atattgccca
     9421 aagcgcaatg gcggcaatta acgtgggagt aaaccaatga ataactctga attagaaaca
     9481 ctgatccgca atattctgcg tgagcagctt gtccccgcta cgcaacgagc gcaaagaaac
     9541 gctattttcc agactgttga tgaagctgtc tgcgcagcac accaggcctt tttacgcttt
     9601 caacagtgcc cgcttaaaac ccgtagcgcc attatcagcg ccattcgcga agaattaatg
     9661 ccccgcctta ccgaactggc agaagaaagc gccaaagaaa cggggatggg aaataaagaa
     9721 gataagtacc taaaaaacaa agcggcactt gataacacgc ctggaattga agatttaacc
     9781 accacagcac tgaccggtga tggaggcatg gttttgtttg aatactctcc gtttggggtg
     9841 attggctcgg tcacgccaag caccaacccc acggaaacca tcattaacaa cagcatcagc
     9901 atgctggcag caggtaatag cgtctacttc agtccacacc cgggagccaa aaacgtttcg
     9961 ttaaaactga ttaccataat cgaagacatc gcgtttcgct gtagtggcat tcacaacctg
    10021 atcgttaccg tatctgaacc aacctttgcc gcaaccgaac aaatgatggc gcaccctaaa
    10081 atcagcgttc tggcgattac cggtggcccg gggatcgtcg ccatgggaat gaaaagtggc
    10141 aagaaagtga ttggtgccgg agcgggtaac ccaccctgca ttgtcgatga aacggcggat
    10201 attgtgaaag ccgccgagga catcatcaac ggcgcttcat ttgattacaa cctgccgtgt
    10261 atcgctgaaa aaagcctgat tgtcgtggta tcagtggcag agtatttaat tcagcagatg
    10321 caaagctttg gagcacttct tcttaaccat gaacaaacgg agaaattgcg tgcaatttgc
    10381 ctgccagacg gtagcgccaa caaaaaactg gttgggaaaa gcccatcagc cttgctggaa
    10441 gcagcgggcc tccctttgcc cgcaaaagcg ccgcgcctac tgattgctgt tgttgacgcc
    10501 gatgatagtt gggttacgtg tgaacaactg atgccaatgc tgcccattgt gaaggtcaac
    10561 gattttgaca gcgctctcgc cctggcattg aaagtggaag acggcttgca ccacacggca
    10621 attatgcact cgcagaacgt ctcacggctc aaccttgccg cgcgcgttat gcaaacctca
    10681 atctttgtca aaaacggccc ctcctgggcg ggtattggcg tcggcggcga aggctttacg
    10741 acctttacca ttgcaacacc aaccggagag ggtacaacgt cagcacggac ttttgcccgc
    10801 tcacgacgct gcgtgttaac cagcggcttt tccattcgct agagaaacgg aaatgaaaac
    10861 tttttcactg cagacgcgtt tatacagcgg aacgggaagt ctcaacatac tgaaccgttt
    10921 cacgaatagg cacatctgga ttatttgcga cgtctttctg gcacactcac cactcattga
    10981 cacgttgcat caggcgctgc ccgacagtaa tcgtctcagt atttttagcg aaataacgcc
    11041 cgacccaact atccagaccg tggtgaaagg cattgcgcaa atgcagactt tacgcccgga
    11101 tgtggttatc ggttttggcg gcggttctgc cctggacgcc gcaaaagcga ttgtctggtt
    11161 cggtcgccag tgtggcattg aaattgaaac ctgcgtagcc attcctacca ccagcggtac
    11221 cggttccgaa gtgaccagtg cttgtgtcat cagcgatcct gagaaaggga ttaaatatcc
    11281 ccttttcgat aatgcacttt atccggatat cgcgatcctt gatccatctt tggtcgtcag
    11341 cgtgccaccg gcgataacgg ccaataccgg gatggatgtt ttaacccatg cgcttgaagc
    11401 ttatgtttct ccccgcgcca gcgacttcac ggatgcactg gtcgaaaaag ccgtgcaaat
    11461 tgtttttcaa tacctgccca ctgcagtgaa gaaaggcgat tgtcttgcca cccgtggcaa
    11521 aatgcataat gcatcgacgc ttgcaggaat agcctttagc caggccggac tcggtttaaa
    11581 ccatgccatt gctcatcaac tcggcggaca gtttcatctg ccgcacggcc tggcgaatgc
    11641 gctcctgctc acggcggtta tccactttaa tgctggcgat ccgcgcgcag ccaaacgcta
    11701 cgcccggctg gcgaaaacgt gccatttatg cccggacaac gccaatgaca ccgccagcct
    11761 gaatgcactt attcaacata ttgagcagct taaaacaacc tgtacgctgc cgactcttac
    11821 caatgcgctg aaagaaaaaa aagcagaatg gtcaatacgc ataccagata tggttcaggc
    11881 ggcactggcg gatgcaacgt tgcgtaccaa cccgcgtgcg gctgatgcct ccgctattgc
    11941 agaactgctc gaggagttgt tatgaacgtc gagttaacct cctgcgacga ccagaccatc
    12001 cgcgagcgag tacaggcagc aggtgtcgtg ggtgctggcg gcgcaggttt tccaacccat
    12061 attaagctac aagcgcgtgt tgacactttt ctggtgaatg cggcggaatg cgaaccaatg
    12121 ttgaaagtgg atcaacagtt aatggtgcag caagccagcc gcctgcttcg cggtgttcac
    12181 tatgcgatga aagccaccgg ggccagtagc ggaatcatcg cgctgaaaga gaaatatcaa
    12241 cgggcaataa acgcactcac ccccctatta ccaccggata tccgcctgca tattttgccc
    12301 gacgtttacc ccgcaggaga cgaagtgctg accatctgga tggcaactgg tcgccgcgtt
    12361 ccacccgctg cattacccgt taacgtcggt gtggtggtca ataatgttca aaccgtactt
    12421 aatattgcca gagccgtcga gcaacagtat ccagtgacgc gccgcacact cactattaat
    12481 ggtgcagtgg cgaaaccaat aacggtaacc gtccccatcg gcatgtcttt acgtgaagtc
    12541 ctggcgctgg cgggcggcgc aacagtagat gctcctggtt ttatcaatgg cgggccgatg
    12601 atgggaaatt tgatcacatc gctcgacacc cccgtcagca aaaccactgg cggcttgctg
    12661 gttttaccca actctcattc actgatccaa cgtcgaatgc agaatgaccg cagcgtactt
    12721 accgtcgcga aaactgtttg tgagcaatgc cgcttgtgta ccgatctctg cccacgtcat
    12781 ttagtcggtc acgaactggc accccattta ttagtacgtg cggttaacta ccaacagctc
    12841 gctacgccac aactgttact gacggcgctc acctgttcgg agtgcaacgt ctgcgccagc
    12901 gtcgcctgtc cggtaggaat ttcaccaatg cgcataaacc gcctgcttaa gcaggaatta
    12961 cgcgcacaaa atctccgcta cgaaggtgcc ttaaatcctg ctgatcccat ggcaaattat
    13021 cgactgattc ccgtaaaacg gctggtaacc agactgggcc taatcccgtg gtatcaggat
    13081 gcgccattgt ctgaacaggt gcctcagccc gaaaaagtga cgttattgtt acgccagcac
    13141 atcggtgcca gcgcaattgc gtgtgtacaa aaaggcgatc gcgtcgttca tgggcaatgt
    13201 gtaggtcaaa ttccacacgg cacattaggt gcccccattc atgccagtat tgatggcatg
    13261 gtcagcgatg tgacagaaaa cgcgattacg cttgtgagag gttaacgatg tcgaaagcgg
    13321 taggtatttt agaacttagc agtatcgcga aagggatgga aaccggcgac gccatgctga
    13381 aaagcgcaaa cgttgagtta ctggtcagca aaacgctatg ccccggaaaa tttctgctga
    13441 tgctcggtgg cgatgtgggt gcggtgcaac aggccatcaa cagcggtgct tcccttgctg
    13501 gtgaaatgct ggtcgacagt cttgtcctgc caaatattca tcccagtctc ctttctgcaa
    13561 tcagcggact gaaccatgtt gaacagcacc gggctgtcgg cgtagtcgaa acctggagtg
    13621 tcgccgcctg cattgacgct gccgattgtg ctgtcaaagc tgcgaatgtc attctcgttc
    13681 gcgtacatat ggcttttggc attggtggta aatgctacat ggtggttgct ggcgatattt
    13741 cagatgttaa taacgcggtc tctgtcgcca gcgagcgtgc gggggaaaaa ggtttgttgg
    13801 tctatcggtc agtgattgcc cgtccacatg aagccatgtg gcgtcagatg gtggagggat
    13861 aatgacaact cagcacacaa ctgaacggat gattcaggaa tatgtccctg gtaagcaggt
    13921 cacgctggca catgtcatcg ccaatcctgg gaaggatctg tttaagaaat tgggtttacc
    13981 agacaccgtt tctgctattg gcattctgac gattaccccc agcgaagcat ctatcatcgc
    14041 atgtgatatc gccacaaaat caggcgctgt agagatcggt tttcttgatc gttttaccgg
    14101 tgccgtagtg ctgactggtg atgtttctgc tattgaatat gcacttagcc aggttacccg
    14161 tacgctgggt gaaatgatgc gctttaccgc ctgccccatt acgcggacct gacgttatgc
    14221 aacgtatgat gcttatcggc cccagccagt gcggaaaaac gtcactcacg caatgtctgc
    14281 gtggcgaaaa tatccattat cagaaaacgc aggcaatcgt ctggtccccg accaccattg
    14341 atacaccagg cgaatacctc gaaaaccgca gcctctacag tgcattgttg gtcagtgcct
    14401 gcgaagcaga cattatcgcg ctggtactta atgcggacgc accgtggtca cctttttcgc
    14461 caggctttac ctcgtcaatg aatcgcccgg tcattggagt agtaaccaaa gcagatttag
    14521 ccagcatgga acaaatatct ttggtgaaat gttggttacg ggaagctgga gcgcacaacg
    14581 tgttagtaac cagtgcggtt aataacaatg gagttacaga actttttgcc ttgctgcata
    14641 cagaagaagg ctgccgctaa tttcgatagg ttctcagatg agaatgatga cggtgataca
    14701 cagataagaa tcaatgagca atgagggttg ccgggcaacc ctcattgaat aaaacaggaa
    14761 ttacttcgct tcgccgtttt tcatttcgcc catagctttc agctttttgg agatatcgcg
    14821 gcgttctttg gagagctcgg catttttgat gatgtaatcg tcaacgcgat cttcatagtc
    14881 acctttcatg ctggcgatga tgccctggat tgcttcaacg ctcatccctg gcttgatgta
    14941 atcgctcagg ttgtccagca gcaggacgcg cttctggttg tcacggatct ttttctcaac
    15001 gtcctgaatt tcacgttgca gtttgttctt acgacggaac agacgaacaa attccagtac
    15061 gtcctggaat gaaggcttgg tagtttccat ttttataccc ctgataatgt gagagtcgga
    15121 ttcgtttaag caaccgctat tcgttagggc caacattact catagctgcc gcagatgaca
    15181 atgcttttat cccttttcac tatcataccc tttatccttg ctgaatcgaa gcagcagcaa
    15241 gatgattctg aagttcagga attatttgcg caaggcccgg caaatcagat tcgatagcaa
    15301 ctcaagctga tgtgccgttt ccatgttcag ccacacataa ccatagattg gtgtttccac
    15361 aaccttcagg tcatggtggt tatcgagcaa ctgacgcagc tcttccacag catcgttcaa
    15421 tttttccgta ttggcaaaaa ccggctgcgg attaccttcg tacagcgcat gaacaaggct
    15481 cagcagtatt tgctgcatca tgtgctgggt atcacgaagt ttttgcgcgt tcaataacac
    15541 gaaatggctg gggcgcgtgg cccagtatgc attgatttgc aactccagca tacaaaccag
    15601 attgcgatta atggtctgga taccttcata tatcgatttt ggaatacggg tttctttgct
    15661 ggcgggcgca atcagcccac gcattttcac ggcatcggtc aggagttttt gtagatggct
    15721 ttccagacgt gggcgttcga gtaagttcgg tgagaatgca gattgataga cccgattata
    15781 ctcggtcaga cttttcgcca gttgaatgcg ccagtggatg aacgcccgtt gtggccagat
    15841 accggtaaac aacattgcca gtaaagagcc gaggatcaca tcgccgcttc gccataacgc
    15901 cgtatcaatt tcacctgtcg gggaacccac aacaattgcc agcgtcaccc caatcaataa
    15961 accttgatat ggtttcttgc ccagcgccag ccaaccgcaa aggaacatgg ccgccgcgca
    16021 ccagactaac atcagcggta acgagattaa ctccagttgc agagcgataa gacctaaaat
    16081 cgaccccaac accgtaccgc caatacgttc gaaggcacga gggacaacgt tcccccagaa
    16141 cgagattggc cccataatca ccaccatggt gaccagcggc caggtgcttt ccgggatagt
    16201 aaacaggcgg ataatgagaa aagtgagcag gaatgccagc gcgacccgag taccatgcac
    16261 aatgcggtaa tggcggtata cccggatttc aaacgggctt aatgacttat cggcacgcac
    16321 gcagaaactc cgtaatgaag attaaaagcg ctcataactc atctgtcggc cagaaggcgc
    16381 gcctctgaat tatccgacgg acaaaccggg aacagtgtaa tttcccggca ggagcgaaaa
    16441 aattgaaagg cgcatcttac tcttttcgct ttcaaaaaaa gagtggtctt cgcgtcaaca
    16501 caccgccctg agatgaatta gtgatgtttt ggctgcaccg caacatacat ttcgatatcc
    16561 cagtacccat cttccgcgcc attgttcaag taaacctcga agcagggctt tggtaacatt
    16621 tcataagcac tgtcctgcaa gaggctatta aagaactgat accagggttt agcaaaatca
    16681 tcaccgacta cacgagccac cgccaccgca tactgaccac ctgaaatttc tgtcagaatg
    16741 acgccctcac tgttttcggg gagcgtaaag ttattcggta ccgtcacgac ggtgtcgcag
    16801 cgtaattttt cggcgggtgt ttcatctgga ttgtcgtaat agacagcaac ccactccttc
    16861 ggcacaatat ttttgctatc tatccacatc atcaactgct caaaaccttt ctttaccgtc
    16921 tgctcccacg ggccaacaag atggaaacct gcaacggtac gtttctcttc ctgcttaatc
    16981 tcgtagttca tgacgcctcc attaacactg tttttatata cagtatagtt gcaatattaa
    17041 aaccacaagg aatgagtgtt gattatgcga gcagactcgc actcctgcca gtctgctgca
    17101 aaagaaaggt caggccttat ggtggaaata atcactcagg cgggaaaaca tgccgccttc
    17161 cccgacagat tccagggtaa ccagcggcca gtgcgccacc tgtttatcac ggtcgtaaag
    17221 ttcaatttcc cctacccgct gatgggcgct aattggcgcg gtgagctctt taccatcaag
    17281 ggtatatttg gctttgatat gtggaatttc ggctttcggt agcaccatcc agaactcttg
    17341 ttccgttccc aggtcgatat tttctttatc gccataccag atgcgttccg ttccgacctt
    17401 tttcccacgg tgcaaaattt gcaccgtagt aaagttttgt tgcccccaac gcagtaattt
    17461 tcttgcctct tcctcacgac cttttgcact gtcagccccc attaccactg caatgagacg
    17521 acgctgccca tctacagccg aagcaatgag attaaacccg gcaccagaag tatgacccgt
    17581 tttcaggccg tcaacattca tggttttatc ccacaacaac ccgttacggt tttgctgggt
    17641 gataccgttc caggtgaggc ttttctcact gtacatatga taaaactcgg gctcgccgtg
    17701 gatgatagcg cgagaaagca cagctaaatc ataagccgag ctatgctggc caggtgcatc
    17761 cagaccatgc actgtttcaa aatgcgtatc cttgagatgc agcttctcgg catagttgtt
    17821 catcattgca acaaactgcc gttgcccacc ggcaatatag tcagccagtg caacacaagc
    17881 atcatttccg gaatccacaa ttaaaccacg gcttaaatca cgtaccgata cgcgatcgcc
    17941 ctctttcaaa aacatcagtg aagaaccgac aaacactgga ttatctttcg cccacgcatc
    18001 gcgccctacg gtgacaatat cgtctggcgt aattcgatga ctatcgatag cgcgatccac
    18061 gacataaccc gtcatcagct ttgtcaggct ggcgggattg cgctgttgat gctcattacc
    18121 cgcggtgagg atctgtccgg tggtgtaatc catcaatacc caggacccgg catgaatctc
    18181 tggaggctga ggtgaaaaag gaatgttttc cgccgcaaaa ccagacgata agttaaaaac
    18241 gaacaaagaa gcagcaataa taagacggcg tttcaacagc aaaccctcag gagtttcaaa
    18301 tagctgttct ttttacggaa atacttatga actggctgga ataaagtgca agaaaatgtg
    18361 actaacctct catttttatc tgacatgatc tgttgccact cgctgccaaa ttgtggcgct
    18421 aaagctgatt agcacggtga tatttgatac tctggcagac agcagagata acggatttaa
    18481 cctaatgatg aatgacggta agcaacaatc tacctttttg tttcacgatt acgaaacctt
    18541 tggcacgcac cccgcgttag atcgccctgc acagttcgca gccattcgca ccgatagcaa
    18601 attcaatgtc atcggcgaac ccgaagtctt ttactgcaag cccgcggatg actatttacc
    18661 ccagcctgga gcagtattaa ttaccggtat taccccgcag gaggcacggg cgaaaggaga
    18721 aaacgaagcc gcgtttgccg cccgtattca ctcgcttttt accgtaccga agacctgtat
    18781 tctgggctac aacaatgtgc gtttcgacga cgaagtcaca cgcaacgttt tttatcgtaa
    18841 tttctacgat ccttacgcct ggagctggca gcatgataac tcgcgctggg atttactgga
    18901 tgttatgcgt gcctgttatg ccctgcgccc ggaaggaata aactggcctg aaaatgatga
    18961 cggtctaccg agctttcgcc ttgagcattt aaccaaagcg aatggtattg aacatagcaa
    19021 cgcccacgat gcgatggctg atgtgtacgc cactattgcg atggcgaaac tggtaaaaac
    19081 gcgtcagcca cgcctgtttg attatctctt tacccatcgt aataaacaca aactgatggc
    19141 gttgattgat gttccgcaga tgaaaccctt ggttcacgtt tccggaatgt ttggggcatg
    19201 gcgcggcaat accagctggg tggcaccgct ggcgtggcat cctgaaaatc gcaatgccgt
    19261 aattatggtg gatttggcag gagacatttc gccattactg gagctggata gcgacacatt
    19321 gcgcgagcgt ttatataccg caaaagccga tcttggcgat aacgccgccg ttccggttaa
    19381 gctggtgcat atcaataaat gtccggtact ggcccaggcg aatacgctac gcccggaaga
    19441 tgccgaccga ctgggaatta atcgtcagca ttgcctcgat aacctaaaaa ttctgcgtga
    19501 aaatccgcaa gtgcgtgaaa aagtggtggc gatattcgcg gaagccgaac cgtttacgcc
    19561 ttcagataac gtggatgcac agctttataa tggctttttc agtgacgccg atcgtgcagc
    19621 aatgaaaatt gtgctggaaa ccgagccgcg taatttaccg gcactggata tcacttttgt
    19681 tgataaacga attgaaaaac tgttgttcaa ttatcgagcg cgcaacttcc cggggacgct
    19741 ggattatgcc gagcagcaac gctggctgga gcaccgccgc caggtcttca cgccagagtt
    19801 tttgcagggc tacgctgatg aattgcagat gctggcacaa cagtatgctg atgacaaaga
    19861 gaaagtggcg ctgttaaaag cgctttggca gtacgcggaa gagatcgtct gaaacaaaaa
    19921 agccggagcg acaaactccg gctttttgag atttaccgga tgattatgtc atccggtttg
    19981 taagattacg ctacgtcttc gtactgcgga accgggttac ggaaactctt ggttacgcaa
    20041 gccaggtaaa tcagaccgat agcagcccag atcagaccca gaaccattga gctttcttcc
    20101 aggttaaccc acagcgcacc aacggtcagc gcaccacaca tcggcaggaa cagatactgg
    20161 aagtgatctt tcagcgtctt gttacgcttc tcacggatcc agaactgcga gatgaccgac
    20221 aggttaacga aggtgaacgc caccaacgca ccaaagttaa tcagcgccgt agccattacc
    20281 aggtcgaagt tgattgccag cagggcaatc gcgccaacca ggatgatgtt catcgccgga
    20341 gtacgccatt tcgggtgtac ataaccgaag aagcttttcg ggaacacgcc gtcacgaccc
    20401 attacgtaca tcagacgcgc tacgcctgcg tgcgccgcca taccggacgc cagtacggta
    20461 atggtggaga agatcagcgc gccaacctgg aacgctttac ccgcaacgta cagcatgatt
    20521 tcaggctgtg acgcatccgg atctttaaag cgagagatat ccgggaagta cagctgcagg
    20581 aagtaagttg caaagatgaa gatcatgccg ccgatcagcg cggtcaggaa aatcgcacgc
    20641 gggatcacgc gctctgcatc tttggtttct tccgacaggt tgctgatgcc gtcaaaaccg
    20701 gtaaaggaga agcacaggat tgtcgccccg gtaatcatcg ggataacatg cgcatcgcca
    20761 gaccagaacg gacgagtgct cgccagcgta ccagcgcctt caccttcaaa tacgccataa
    20821 acaaccatgc ccagaatcac cgcgatcagc actacctgca acacgacgat tacagtgttg
    20881 aagttcgcta cggatttcag actacgcagg ttaaaggcgg tcataaaggc caccagcgcc
    20941 accacaaaca tccacgatgg aatggaaggc accagagctt caaaatagat tttcgccagc
    21001 agaatgttga tcatcggcgc gaacagatag tcgagcagag aagaccaacc caccataaag
    21061 ccgacagtcg ggctaatgga tttctgggcg taagtgtatg cagagccagc agaaggatag
    21121 cggcgaacca gcttcccgta gctcagagcc gtaaacagga tcgcaatcaa tgcgaacgca
    21181 taggctgtcg gcacatgacc atccgtgagg cctgaaacga taccaaaagt atcaaacagc
    21241 gtcatcggct gcatataggc tagacccatc attacaaccg gaactaacgt aagcgtttta
    21301 cgcaattcca cgcgagaggt gtttggagta acgttatgcg acatggtcat tctcctttac
    21361 ggtgataacc gtcgcgtaag caaaaaattg ccccattttt ttggattcct cagcgacaac
    21421 aactgtcgat ttttagtaaa tatctatccg gtacgaagcc cggcctcttg gtatgaatga
    21481 ttggtttgaa gcaaaaaata accgacgcta attaaacgtc ggtttttagt cattttttga
    21541 cagcggcgca ttgtgcctaa atggggggga aatgacaaga gaatgagagg cttgtcataa
    21601 taatttttct ttaaatggct gattttccgt catcagattt tgcataaaca ccggcgatcg
    21661 cactatttgc taaaatttcg tcccgccacc aaccactggc aagccctgcg gtctgttcat
    21721 tccagccaat ccagacgggt tcaaagagag tttgggcgac cactttcttc tcgattaacg
    21781 cgccactatc gagaaaacgt tgcgccagat accgtggtaa atagccgcag ccaaggccgc
    21841 taatttgcag ctccagcttg gttttaaaat cgaaaacggt aatggcttct tgttcgtcga
    21901 gaagttgtga ggccgtagaa gcgcctgcct gcgcagtgtc ccccaccaca atcgcccgat
    21961 atcgtttaat gatacgacgg tttaacggtt cttcttcctg ggctaacgga tgatgaggcg
    22021 cgaccgcaaa gacttgttca agatcgccca accgcgagaa accaaactca ctggaagatg
    22081 gtggctcatg catcgcgcca acaataatat ccgctcgccc ctgagtcagt gcatcccagg
    22141 aaccaccgag tacgccattg ataaacttca ggcgcgtcac gctatgatgt tgataaaaag
    22201 cctcaatgag aggcgcaagc agagaaaaag gaaacgtgtc gtccacgcca atcaccagct
    22261 cattttccca gccttcatgc agtttgatag cctgcttttc cagttctcgc acggtatgca
    22321 gaacttcccg ccctttctct aataacattt ttccggtgcg ggtgaattta gcgcggtgac
    22381 cgctacgatc aagcaattga atattaaggt cgctttccag cttgtgaacg gtataactca
    22441 gagcggacgg cgtcttgtag agtttagccg acgccgccgc aaaactgcct tctttttcta
    22501 aggcatcgag gatcatcaac acatccagca gtggtttcat acacgccccc ttgcagtgta
    22561 tgcggcgtcc tgctggcgtg atcgtttact ccagcggcat taccagcgga tcgggatact
    22621 ggtattcaaa tcccagttca ttacaaatcc gactgccatc aataatctta cctttgccgc
    22681 tgtccagact atttctgaac tgcggaggtt ccagccccag taaacgggcc atctgcggat
    22741 agaaaacatt acgcgcaggg tgagctggcg cacatatatt atagatgtgt ccgcctttag
    22801 gtgcctgtaa caacagagtg atagcaccaa taacatcttc taaatggact aaattaacac
    22861 catgttcacc atcaggggcg gtttttccgg caaagaagcg tccgggatga cgtcccggtc
    22921 cgaccagccc cgcaagacgc agaatatcga ccgaagtacc gggtaaattg tgcagccagt
    22981 cttcgagttc ttctaatact cgtccactgt tggtcaccgg attacgcggg gtggtttctt
    23041 tcaccgtgcc ttgcgcgtcg ccatagacag atgttgagct ggtaaaaata attcggggaa
    23101 tacgatgggc cagcgcgcta tccactaact cttgtaccgc ttgtaaatag aactcatcgc
    23161 cggggccgct acgacgtgcc ggaagcgtaa tgaccagcgc gtcggcatcc atcagggcat
    23221 ccagatcgtc agaatcgcaa actaactcag gctccatgcg aagcagatag ctatcaatgc
    23281 cactcattcg ggccgcttcg acaccatctt gtgtggtttt actcccggtg acttgccagc
    23341 ctcgcgctga aagtgacatc gccagcggca tgcctaacca ccctaacccg acaattgcga
    23401 cctttttcat ccgttatctc ctgatacacc ttagatctat aaggctacgc tagcgtatca
    23461 aaactgacaa ttcattctat gaatgaatct gttcaataat gataacgaca tgctgcaatg
    23521 agtagtgaat cgtcggtaac cgcgtatacc agacggtgct cctctgtaat gcgtcgggac
    23581 cagaaacctg acaaattatg tttcaggggt tctggtttcc ccttaccttc aaatggtgtt
    23641 ctgcgggtat ctttgataag ttcattgatc tttttaacaa ttcgcttatc tgtttcctgc
    23701 cagtacagat aatcatccca tgattcctca gaccagatta gtttcactca atgatgtcct
    23761 tttccgttcc tttgccagat ttcaggctat cgattgagtc catcaatctc cgggcgttag
    23821 cgggggagcg cagtagataa gccgtctcct ccagggagtt gtattcttcg agtgacatca
    23881 gaacacaagc ctctccattc tgacgagtaa taaggatcgg ggcatgatct tcaacggctt
    23941 tcatcattgt tgccgacaaa ttctgacgcg cttcgctgta gctaattgta cgcatgtcaa
    24001 tctcctcttt tgtacagttc attgtacaat gatgggcgtt aattaactat ttattaatta
    24061 gtttgtagat caaggtattg tcagtgagac gaaaatccag gcttcgctat ttttggtgcc
    24121 gtcagcttag aggacagtcc tcttagcccc ctcctttccc cgctcattca tt
//
LOCUS       DAZFLD010000061        24011 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.061, whole genome shotgun sequence.
ACCESSION   DAZFLD010000061 DAZFLD010000000
VERSION     DAZFLD010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24011)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24011)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24011
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.061"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            147..2390
                     /gene="pdeF"
                     /locus_tag="ACHKBL_002041"
     CDS             147..2390
                     /gene="pdeF"
                     /locus_tag="ACHKBL_002041"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HFO5818918.1"
                     /translation="MKLNATYIKIRDKWWGLPLFLPSLILPIFAHINTFAHISSGEVF
                     LFYLPLALMISMMMFFSWAALPGIALGIFVRKYAELGFYETLSLTANFIIIIILCWGG
                     YRVFTPRRNNVSHGDSRLISQRLFWQIVFPATLFLILFQFAAFVGLLASRENLVGVMP
                     FNLGTLINYQALLVGNLIGVPLCYFIIRVVRNPFYLRSYYSQLKQQVDAKVTKKEFAI
                     WLLALGALLLLLCMPLNEKSTIFSTNYTLSLLLPLMMWGAMRYGYKLISLLWAVVLMI
                     SIHSYQNYIPIYPGYTTQLTITSSSYLVFSFIVNYMAVLATRQRAVVRRIQRLAYVDP
                     VVHLPNVRALNRALRDAPWSALCYLRIPGMEMLVKNYGIMLRIQYKQKLSHWLSPLLE
                     PGEDVYQLSGNDLALRLNTESHQERITALDSHLKQFRFFWDGMPMQPQIGVSYCYVRS
                     PVNHIYLLLGELNTVAELSIVTNAPENMQRRGAMYLQRELKDKVAMMNRLQRALEHNH
                     FFLMAQPITGMRGDVYHEILLRMKGENDELISPDSFLPVAHEFGLSSSIDMWVIEHTL
                     QFMAENRAKMPAHRFAINLSPTSVCQARFPVEISQLLAKYQIEAWQLIFEVTESNALT
                     NVKQAQITLQHLQELGCQIAIDDFGTGYASYARLKNVNADLLKIDGSFIRNIVSNSLD
                     YQIVASICHLARMKKMRVVAEYVENEEIREAVLSLGIDYMQGYLIGKPQPLIDTLNEI
                     EPIRESA"
     gene            complement(2429..3970)
                     /gene="ppx"
                     /locus_tag="ACHKBL_002042"
     CDS             complement(2429..3970)
                     /gene="ppx"
                     /locus_tag="ACHKBL_002042"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004309 - exopolyphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006798 - polyphosphate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HFO5818919.1"
                     /translation="MPIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQR
                     VHLADGLGPDNMLSEEAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFL
                     KRAEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPI
                     LVESRRMGCVSFAQLYFPGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASG
                     TIKAAHEVLMEMGEKDGIITPERLEKLVKEVLRHRNFASLSLPGLSEERKTVFVPGLA
                     ILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQAR
                     RVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNS
                     DLPGFNQEQQLMMATLVRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQ
                     ATTTPPTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEEST
                     PEIAA"
     gene            complement(3975..6041)
                     /gene="ppk1"
                     /locus_tag="ACHKBL_002043"
     CDS             complement(3975..6041)
                     /gene="ppk1"
                     /locus_tag="ACHKBL_002043"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HFO5818920.1"
                     /translation="MGQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSN
                     NLDEFYKVRFAELKRRIIISEEQGSNSHSRHLLGKIQSRVLKADQEFDGLYNELLLEM
                     ARNQIFLINERQLSVNQQNWLRHYFKQYLRQHITPILINPDTDLVQFLKDDYTYLAVE
                     IIRGDTIRYALLEIPSDKVPRFVNLPPEAPRRRKPMILLDNILRYCLDDIFKGFFDYD
                     ALNAYSMKMTRDAEYDLVHEMEASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVEVL
                     REKLTISRYDSIVPGGRYHNFKDFINFPNVGKANLVNKPLPRLRHIWFDKAQFRNGFD
                     AIRERDVLLYYPYHTFEHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHN
                     GKKVTVVVELQARFDEEANIHWAKRLTEAGVHVIFSAPGLKIHAKLFLISRKENGEVV
                     RYAHIGTGNFNEKTARLYTDYSLLTADARITNEVRRVFNFIENPYRPVTFDYLMVSPQ
                     NSRRLLYEMVDREIANAQQGLPSGITLKLNNLVDKGLVDRLYAASSSGVPVNLLVRGM
                     CSLIPNLEGISDNIRAISIVDRYLEHDRVYIFENGGDKKVYLSSADWMTRNIDYRIEV
                     ATPLLDPRLKQRVLDIIDILFSDTVKARYIDKELSNRYVPRGNRRKVRAQLAIYDYIK
                     SLEQPE"
     gene            complement(6212..6850)
                     /gene="purN"
                     /locus_tag="ACHKBL_002044"
     CDS             complement(6212..6850)
                     /gene="purN"
                     /locus_tag="ACHKBL_002044"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HFO5818921.1"
                     /translation="MNIVVLISGNGSNLQAIIDACKTNKIKGTVRAVFSNKADAFGLE
                     RARQAGIATHTLIASAFDSREAYDRELIHEIDMYAPDVVVLAGFMRILSPAFVSHYAG
                     RLLNIHPSLLPKYPGLHTHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGD
                     TEDDITARVQTQEHAIYPLVISWFADGRLKMHENAAWLDGQRLPPQGYAADE"
     gene            complement(6850..7887)
                     /gene="purM"
                     /locus_tag="ACHKBL_002045"
     CDS             complement(6850..7887)
                     /gene="purM"
                     /locus_tag="ACHKBL_002045"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HFO5818922.1"
                     /translation="MTDKTSLSYKDAGVDIDAGNALVGRIKGVVKKTRRPEVMGGLGG
                     FGALCALPQKYREPVLVSGTDGVGTKLRLAMDLKRHDTIGIDLVAMCVNDLVVQGAEP
                     LFFLDYYATGKLDVDTASAVISGIAEGCLQSGCSLVGGETAEMPGMYHGEDYDVAGFC
                     VGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVSGCDPQTTELDGKPL
                     ADHLLAPTRIYVKSVLKLIEKVDVHAIAHLTGGGFWENIPRVLPDNTQAVIDESSWQW
                     PEVFNWLQTAGNVERHEMYRTFNCGVGMIIALPAPEVDKALALLNANGENAWKIGIIK
                     ASDSEQRVVIE"
     gene            8212..8838
                     /gene="upp"
                     /locus_tag="ACHKBL_002046"
     CDS             8212..8838
                     /gene="upp"
                     /locus_tag="ACHKBL_002046"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HFO5818923.1"
                     /translation="MKIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYEAT
                     ADLETEKVTIEGWNGPVEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGMY
                     RNEETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLV
                     AAPEGIAALEKAHPDVELYTASIDQGLNEHGYIIPGLGDAGDKIFGTK"
     gene            8924..10213
                     /gene="uraA"
                     /locus_tag="ACHKBL_002047"
     CDS             8924..10213
                     /gene="uraA"
                     /locus_tag="ACHKBL_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HFO5818924.1"
                     /translation="MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPA
                     TVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFC
                     LVSFIVKKAGTGWLDVLFPPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQTPDSKTII
                     ISITTLAVTVLGSVLFRGFLAIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTL
                     YTPRFEWFAILTILPAALVVIAEHVGHLVVTANIVKKDLLRDPGLHRSMFANGLSTVI
                     SGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVM
                     GGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVNIGAAELKGM
                     ALATIVGIGLSLIFKLISMLRPEEVVLDAEDADITDK"
     gene            10308..11009
                     /gene="hda"
                     /locus_tag="ACHKBL_002048"
     CDS             10308..11009
                     /gene="hda"
                     /locus_tag="ACHKBL_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA inactivator Hda"
                     /protein_id="HFO5818925.1"
                     /translation="MNTPAQLSLPLYLPDDETFASFWPGDNSSLLAALQNVLRQEHSG
                     YIYLWAREGAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVPEVLDGMEHLSLVCID
                     NIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNLGLPDLASRLDWGQIY
                     KLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRTLFMTLDQLDRASITA
                     QRKLTIPFVKEILKL"
     gene            complement(11115..11474)
                     /gene="arsC"
                     /locus_tag="ACHKBL_002049"
     CDS             complement(11115..11474)
                     /gene="arsC"
                     /locus_tag="ACHKBL_002049"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311384.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA];
                     GO_process: GO:0046685 - response to arsenic-containing
                     substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HFO5818926.1"
                     /translation="MTKQVKIYHNPRCSKSRETLNLLKENGVEPEVVLYLETPADAAT
                     LRDLLKMLGMNSARELMRQKEDLYKELNLADSSLSEEALIQAMVDNPKLMERPIVVAN
                     GKARIGRPPEQVLEIVG"
     gene            complement(11495..12958)
                     /gene="bepA"
                     /locus_tag="ACHKBL_002050"
     CDS             complement(11495..12958)
                     /gene="bepA"
                     /locus_tag="ACHKBL_002050"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-barrel assembly-enhancing protease"
                     /protein_id="HFO5818927.1"
                     /translation="MFRQLKKNLVATLIAAMTIGQVAPAFADSADTLPDMGTSAGSTL
                     SIGQEMQMGDYYVRQLRGSAPLINDPLLTQYINSLGMRLVSHANSVKTPFHFFLINND
                     EINAFAFFGGNVVLHSALFRYSDNESQLASVMAHEISHVTQRHLARAMEDQQRSAPLT
                     WVGALGSILLAMASPQAGMAALTGTLAGTRQGMISFTQQNEQEADRIGIQVLQRSGFD
                     PQAMPTFLEKLLDQARYSSRPPEILLTHPLPESRLADARNRANQMRPMVVQSSEDFYL
                     AKARTLGMYNSGRNQLTSDLLDEWAKGNVRQQRAAQYGRALQAMEANKYDEARKTLQP
                     LLAAEPGNAWYLDLATDIDLGQNKANEAINRLKNARDLRTNPVLQLNLANAYLQGGQP
                     QEAANILNRYTFNNKDDSNGWDLLAQAEAALNNRDQELAARAEGYALAGRLDQAISLL
                     SSASSQVKLGSLQQARYDARIDQLRQLQERFKPYTKM"
     gene            13171..14232
                     /gene="yfgO"
                     /locus_tag="ACHKBL_002051"
     CDS             13171..14232
                     /gene="yfgO"
                     /locus_tag="ACHKBL_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFO5818928.1"
                     /translation="MLEMLMQWYRRRFSDPEAIALLVILVAGFGIIFFFSGLLAPLLV
                     AIVLAYLLEWPTVRLQSIGCSRRWATSIVLVVFVGILLLMAFVVLPIAWQQGIYLIRD
                     MPGMLNKLSDFAATLPRRYPALMDAGIIDAMAENMRSRMLTMGDSVVKISLASLVGLL
                     TIAVYLVLVPLMVFFLLKDKEQMLNAVRRVLPRNRGLAGQVWKEMNQQITNYIRGKVL
                     EMIVVGIATWLGFLLFGLNYSLLLAVLVGFSVLIPYIGAFVVTIPVVGVALFQFGAGT
                     EFWSCFAVYLIIQALDGNLLVPVLFSEAVNLHPLVIILSVVIFGGLWGFWGVFFAIPL
                     ATLIKAVIHAWPDGQIAQD"
     gene            complement(14331..14801)
                     /gene="bcp"
                     /locus_tag="ACHKBL_002052"
     CDS             complement(14331..14801)
                     /gene="bcp"
                     /locus_tag="ACHKBL_002052"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HFO5818929.1"
                     /translation="MNPLKAGDIAPKFSLPDQDGEQVNLTDFQGQRVLVYFYPKAMTP
                     GCTVQACGLRDNMDELKKAGVDVLGISTDKPEKLSRFAEKELLNFTLLSDEDHQVCEQ
                     FGVWGEKSFMGKTYDGIHRISFLIDADGKIEHVFDDFKTSNHHDVVLNWLKEHA"
     gene            complement(14801..15373)
                     /gene="gcvR"
                     /locus_tag="ACHKBL_002053"
     CDS             complement(14801..15373)
                     /gene="gcvR"
                     /locus_tag="ACHKBL_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     repressor"
                     /protein_id="HFO5818930.1"
                     /translation="MTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAML
                     GEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIVMKRTTARPRPPMPASVWVQVDV
                     ADSPHLIERFTALFDAHHMNIAELVSRTQPAENERAAQLHIQITAHSPASADAANIEQ
                     AFKALCTELNAQGSINVVNYSQHDEQDGVK"
     gene            15519..16397
                     /gene="dapA"
                     /locus_tag="ACHKBL_002054"
     CDS             15519..16397
                     /gene="dapA"
                     /locus_tag="ACHKBL_002054"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HFO5818931.1"
                     /translation="MFTGSIVAIVTPMDEKGNVCRASMKKLIDYHVASGTSAIVSVGT
                     TGESATLNHDEHADVVMMTLELADGRIPVIAGTGANATAEAISLTQRFNDSGIVGCLT
                     VTPYYNRPSQEGLYQHFKAIAEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGI
                     KEATGNLTRVNQIKELVSDDFVLLSGDDASALDFMQLGGHGVISVTANVAARDMAQMC
                     KLAAEGHFAEARVINQRLMPLHNKLFVEPNPIPVKWACKELGLVATDTLRLPMTPITD
                     SGRETVRAALKHAGLL"
     gene            16414..17448
                     /gene="bamC"
                     /locus_tag="ACHKBL_002055"
     CDS             16414..17448
                     /gene="bamC"
                     /locus_tag="ACHKBL_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HFO5818932.1"
                     /translation="MAYSVQKSRLAKVAGVSLVLLLAACSSDSRYKRQVSGDEAYLEA
                     APLAELHAPAGMILPVTSGDYAIPVTNGSGAVGKALDIRPPAQPLALVSGARTQFTGD
                     TASLLVENGRGNTLWPQVVSVLQAKNYTITQRDDAGQTLTTDWVQWNRLDEDEQYRGR
                     YQISVKPQGYQQAVTVKLLNLEQAGKPVADAASMQRYSTEMMNVISAGLDKSATDAAN
                     AAQNRASTTMDVQSAADDTGLPMLVVRGPFNVVWQRLPAALEKVGMKVTDSTRSQGNM
                     AVTYKPLSDSEWHELGASDPGLASGDYKLQVGDLDNRSSLQFIDPKGHTLTQSQNDAL
                     VAVFQAAFSK"
     gene            17661..18374
                     /gene="purC"
                     /locus_tag="ACHKBL_002056"
     CDS             17661..18374
                     /gene="purC"
                     /locus_tag="ACHKBL_002056"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HFO5818933.1"
                     /translation="MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFD
                     RKGMVNNKFNYFIMSKLAEAGIPTQMERLLSDTECLVKKLDMVPVECVVRNRAAGSLV
                     KRLGIEEGIELNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKAND
                     VLKKLFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSL
                     GGLIEAYEAVARRLGVQLD"
     gene            18542..19405
                     /gene="ypfJ"
                     /locus_tag="ACHKBL_002057"
     CDS             18542..19405
                     /gene="ypfJ"
                     /locus_tag="ACHKBL_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HFO5818934.1"
                     /translation="MRWQGRRESDNVEDRRNSSGGPSMGGPGFRLPSGKGGLILLIVV
                     LVAGYYGVDLTGLMTGQPVSQQQSTRSISPNEDEAAKFTSVILATTEDTWGQQFEKMG
                     KTYQQPKLVMYRGMTRTGCGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFA
                     QGYVIAHEVGHHVQKLLGIEPKVRQLQQNATQAEVNRLSVRMELQADCFAGVWGHSMQ
                     QQGVLETGDLEEALNAAQAIGDDRLQQQSQGRVVPDSFTHGTSQQRYSWFKRGFDSGD
                     PAQCNTFGKSI"
     gene            19420..21435
                     /gene="tmcA"
                     /locus_tag="ACHKBL_002058"
     CDS             19420..21435
                     /gene="tmcA"
                     /locus_tag="ACHKBL_002058"
                     /EC_number="2.3.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cytosine(34) acetyltransferase TmcA"
                     /protein_id="HFO5818935.1"
                     /translation="MAELTALHTLTAQMKREGIRRLLVLSGEEGWCFDHALKLRDALP
                     GDWLWISPQPDAENHCSPSALQTLLGREFRHAVFDARHGFDAAAFAALSGTLKAGSWL
                     VLLLPVWEEWENQPDADSLRWSDCPDPIATPHFVQHLKRVLTANNDAILWRQNQPFTL
                     AHFTPRTDWHPATGAPQPEQQQLLQQLLTMPLGVAVVTAARGRGKSALAGQLISRIAG
                     SAIVTAPAKAATYVLAQFAGEKFRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLV
                     SRFPRTLLTTTVQGYEGTGRGFLLKFCAHFPHLHRFELQQPIRWAQGCPLEKMVSEAL
                     VFDDENFTHTPQGNIVISAFEQTLWRSEPQTPLKVYQLLSGAHYRTSPLDLRRMMDAP
                     GQHFLQAAGENAIAGALWLVDEGGLSQELSQAVWAGFRRPRGNLVAQSLAAHGSNPLA
                     ATLRGRRVSRIAVHPARQREGTGQQLIAGALQYTHDLDYLSVSFGYTGELWRFWQRCG
                     FVLVRMGNHREASSGCYTAMALLPMSDAGKQLAEREHYRLRRDAQALAKWNGETLPVD
                     PLNDIVLSADDWLELAGFAFAHRPLLTSLGCLMRLLQTSEMALPALRGRLQKNASDAQ
                     LCTTLKLSGRKLLLVRQREEAAQALYALDDVRTERLRDRITQWQFFH"
     gene            21509..22207
                     /gene="ypfH"
                     /locus_tag="ACHKBL_002059"
     CDS             21509..22207
                     /gene="ypfH"
                     /locus_tag="ACHKBL_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFO5818936.1"
                     /translation="MKHDHFVVQSPDKPAQQLLLLFHGVGDNPVAMGEIGSWFAPLFP
                     DALVVSVGGAEPSGNPAGRQWFSVQGITEDNRQARVDAIMPTFIETVRYWQKQSGVGA
                     TATALIGFSQGAIMALESIKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDP
                     VIDLAHAVAAQEALISAGGDVTLDIVEDLGHAIDNRSMQFALDHLRYTIPKHYFDEAL
                     SGGKPGDDDVIEMM"
     gene            complement(22288..22488)
                     /gene="ypfN"
                     /locus_tag="ACHKBL_002060"
     CDS             complement(22288..22488)
                     /gene="ypfN"
                     /locus_tag="ACHKBL_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpfN family protein"
                     /protein_id="HFO5818937.1"
                     /translation="MDWLAKYWWILVIVFLVGVLLNVIKDLKRVNHKKFLANKPELPP
                     HRDFNDKWDDDDDWPKKDQPKK"
     gene            complement(22516..23643)
                     /gene="dapE"
                     /locus_tag="ACHKBL_002061"
     CDS             complement(22516..23643)
                     /gene="dapE"
                     /locus_tag="ACHKBL_002061"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HFO5818938.1"
                     /translation="MSCPVIELTQQLIRRPSLSPDDAGCQALLIERLQAIGFTVERMD
                     FADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKG
                     SLAAMVVAAERFVAQHPNHTGRLAFLITSDEEASAHNGTVKVVEALMARNERLDYCLV
                     GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIE
                     WDQGNEFFPATSMQIANIQAGTGSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLE
                     KHQLRYTVDWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMG
                     AQVVELGPVNATIHKINECVNAADLQLLARMYQRIMEQLVA"
     gene            complement(23647..24003)
                     /gene="yffB"
                     /locus_tag="ACHKBL_002062"
     CDS             complement(23647..24003)
                     /gene="yffB"
                     /locus_tag="ACHKBL_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HFO5818939.1"
                     /translation="MVTLYGIKNCDTIKKARRWLEANNIDYRFHDYRVDGLDSELLNG
                     FINELGWEALLNTRGTTWRKLDETTRNKITDAASAATLMTEMPAIIKRPLLCAPGKPM
                     LLGFSESSYQQFFHEV"
BASE COUNT         5597 a         6002 c         6323 g         6089 t
ORIGIN      
        1 tttgtttgat atatatcaat tggaactttc atatgacact tagaatcatt gctctctata
       61 gtgatgaata atcatcattc gaagtcaggt gggatgtctg tctgaataca cctccttcag
      121 gatgtggggg attctgctga gcatctatga aactgaatgc aacttatata aaaatacgtg
      181 ataaatggtg ggggcttccg ctgttcctgc cttctttaat cttgcccatt ttcgcccaca
      241 ttaatacttt cgcgcatatt tcttccggtg aagtttttct cttttatctg ccactggcac
      301 tgatgatcag catgatgatg tttttcagct gggcggcatt gccagggatc gccttaggga
      361 tttttgtccg caaatatgca gagctgggtt tttacgaaac gctctcatta acggctaatt
      421 ttattatcat tatcattctc tgttggggcg gttacagggt ttttactccc cggcgtaaca
      481 acgtttcaca tggtgatagc cgtttaattt cccagcgact attctggcag attgtgtttc
      541 ctgcaacgct gtttctgata cttttccagt ttgctgcgtt tgttggatta ctggcgagca
      601 gagaaaatct ggtcggcgtc atgcccttta acctcgggac cttaatcaat tatcaggcct
      661 tgctggtggg taatctgatt ggtgtcccgc tgtgctactt catcattcgg gtggtgcgaa
      721 atccgtttta tttacgtagc tattattcgc aattaaaaca gcaggttgat gccaaagtta
      781 ccaaaaaaga gttcgcaatc tggctactgg cattaggtgc tttactattg ctgttatgca
      841 tgccgttaaa tgaaaaaagc acgattttta gcaccaatta caccttgtca ttattgctgc
      901 ccctgatgat gtggggagcg atgcgctatg gttataagct gatttcattg ctctgggcgg
      961 tcgtgttgat gatcagcatc cacagctatc aaaattacat tcccatttat cctggctata
     1021 ccacgcagct gaccataacc tcctccagtt atctggtatt ctcttttatt gtcaattata
     1081 tggctgtact ggcaacccgt cagcgagcgg tagtcagacg cattcagcgg cttgcgtatg
     1141 tggacccggt ggttcatctg ccaaatgttc gcgccctgaa tcgcgcgtta cgtgatgccc
     1201 cctggtctgc gctttgttat ttacgcatcc ctggcatgga aatgctggtt aagaattatg
     1261 gcatcatgct gcggattcaa tacaagcaaa aactttctca ctggctgtca ccattgctgg
     1321 aaccgggtga agatgtttat cagctttcgg gtaacgatct cgcgctgcgg ctgaatacag
     1381 aatcgcacca ggagcgcatt accgcactgg atagccatct caagcaattt cgtttctttt
     1441 gggatggaat gccgatgcaa ccgcagattg gcgtcagtta ctgctatgtg cgctcgccag
     1501 tgaatcatat ctacctgctg ctgggagagc taaatacagt ggccgaactt tctatcgtga
     1561 ccaacgcccc ggaaaatatg cagcgtcgcg gagcaatgta tttgcaacgc gaattgaaag
     1621 ataaagtcgc gatgatgaat cggctacagc gggcgctgga acacaaccat tttttcctga
     1681 tggcccagcc gattaccggt atgcgtggtg atgtctacca tgaaattctt ctgcgcatga
     1741 aaggtgagaa tgatgaactg atcagccccg atagcttctt accggtcgcg cacgaatttg
     1801 gtttatcgtc gagtatcgac atgtgggtca ttgagcatac gctgcaattt atggctgaaa
     1861 acagagcgaa gatgcccgct caccgttttg cgattaatct gtcgccaacc tcggtatgtc
     1921 aggctcgttt tcctgttgaa atcagtcagc tgctggctaa atatcagatt gaagcgtggc
     1981 aacttatttt tgaagtcacc gaaagtaatg ctctgaccaa tgttaagcag gcgcaaatca
     2041 ccttgcagca tcttcaggaa ttaggctgcc agattgcgat tgatgatttc ggcaccggct
     2101 acgccagcta tgcgcggctt aaaaatgtga atgccgatct gcttaaaatt gacggcagtt
     2161 ttatccgcaa tattgtgtca aatagtctgg attatcagat agtggcgtcg atttgccacc
     2221 tggcgcgaat gaagaaaatg cgggtagtgg cagagtacgt tgaaaacgaa gagatccgcg
     2281 aggcggtgct ctctttgggg atcgattata tgcagggtta tcttattggt aagccgcaac
     2341 cgttaattga tacgctgaat gaaatcgaac ccattcgcga aagtgcctga ataatgcggg
     2401 ccgacatttc tcgtcggccc gcaaagcatt aagcggcgat ttctggtgta ctttcttctt
     2461 caattttcaa ccgccagcca gccacgcctt cccagtattc ttgctccttt tccagatcaa
     2521 gcagtaccag cgcattctga ctaaaccagt catgcggaaa acgcagtgtc cagtgactgt
     2581 catcggtaat cagcgtcaat gttggcggtg tggtggttgc ctgacgttga ttattgagta
     2641 atacgccaag gcgcaatagc tgaatcagtg gcaggaactg tttcttctta aacaaggtaa
     2701 agcgcggcag atcgtcgagc ttaatcgctt tacggtgata gcgcaccagc gtcgccatca
     2761 tcaactgctg ttcctgatta aaacccggca agtcgctgtt ttgcagaata taagcggagt
     2821 ggcgatgcaa accgctgtgg ttgatattca acccgacctc atgcagcatg gcggcccatc
     2881 gcagtagcgc ttccagttgc ggatgcgcca gcttcggttg ctgttcccgc cactgttcgt
     2941 acatttgcat agtggtatcc agcacccgtc gggcctgttc gctgtcgatg tgatactggt
     3001 tggcgaggct gctggcggtg cgactacgca catcctgatg acggaaacgt ccttccattt
     3061 catacagtac gccttcgcgt aacgccccgt cagaaaggcg cagttcacgg atggctaaag
     3121 catcaaacac accgcataaa atcgccagtc caggaacgaa gactgttttc cgttcttcgg
     3181 aaagacctgg taagctcagc gatgcgaaat tacggtgccg taaaacttct tttaccagtt
     3241 tttccagacg ttccggggta attatcccgt ctttctcgcc catttccatt aacacttcat
     3301 gggcggcttt tattgtaccg gaagcgccca ttgcaacatt ccagccctga attcgaaatt
     3361 gccaggttaa agtttccagt ttttgtgctg ccgccatgcg agcgcgctga aaattctctt
     3421 tattgatgac cccgccaggg aaataaagct gggcaaagct gacacaaccc atccggcggc
     3481 tttcaacgag aatcggttcg aaattttcac caatcaccag ttccgtagat ccgccgccaa
     3541 tatcaataac cagtttgcga cctttctccg gttgggtatg ttccacgccc ataaaaatca
     3601 gacgggcttc ttcattaccg gaaataattt caatcgggta gggaatgacc ttttccgcgc
     3661 gtttcagaaa gtcagtggcg ttcagcgcct gacgcagtgt atgggtgcca actatacaga
     3721 cgctggcagg agaaaaccct tgcagccgtt cggcaaacag cgacagacag tttaaaccgc
     3781 gcgtcattgc ctcttcactc aacatattat ctggccccag gccgtccgcc agatgcactc
     3841 gctgtttcag gcggccaata atctgcatgg caccatctac cacacgggct atgaccatgt
     3901 gaaaactgtt tgaaccaaga tcgaccgcag caaactcctg cggacgaggg gatttatcgt
     3961 gtattggcat agggttattc aggttgttcg agtgatttga tgtagtcgta aatcgccaac
     4021 tgcgcccgta ctttacggcg attgccgcgg ggaacgtagc gattactgag ttctttatcg
     4081 atataacgtg ctttgaccgt atcgctgaac aatatgtcga tgatgtccag cacccgctgc
     4141 ttcaggcgcg gatcgagcag cggtgtcgcc acttcaatac gataatcaat attgcgcgtc
     4201 atccagtcgg cggaagaaag gtagaccttt ttatcgccgc cattttcaaa aatataaacc
     4261 cggtcatgtt caaggtaacg gtcaacaata ctgatggcac gaatgttgtc gctaatgcct
     4321 tccagattgg ggatcagcga acacatgccg cgaaccagca gattaaccgg tacgccggag
     4381 ctggaggccg catacagacg atcaaccagg cctttatcga caaggttatt tagcttcagg
     4441 gtgataccgc tgggcagccc ttgctgcgcg ttggcgattt cgcggtccac catttcatac
     4501 aacaggcggc gggagttttg cggcgacacc attaaataat caaatgtcac cggacggtat
     4561 gggttttcaa taaagttgaa cacccgccgt acttcgttgg ttatgcgcgc atcggcggtc
     4621 agcaacgaat agtcagtata aagacgcgcg gttttttcgt taaagttccc ggtcccgatg
     4681 tgtgcgtaac gcaccacttc accgttttct ttacgtgaaa tcaggaacag tttggcgtga
     4741 attttcagcc ccggcgcaga gaagataacg tgcacgcctg cttcggtcag gcgcttcgcc
     4801 cagtgaatgt tggcttcttc gtcgaaacgc gcctgtaact caaccaccac ggtgactttc
     4861 ttaccgttgt gtgcggcgtg gatcatcgag tcgatgatgc gtgaatcttt cgccacgcgg
     4921 taaatgttaa ttttaatcgc cagtacgctc gggtcgaacg aagcctgacg cagcagttcc
     4981 agtacatgct caaaggtatg ataaggataa tagagcaaca catcgcgttc gcgaatagca
     5041 tcaaaaccat tgcggaactg ggctttatca aaccaaatgt ggcgtaaacg cggcagtggt
     5101 ttgttcacca gattggcttt gccgacattg gggaaattaa taaagtcttt aaaattatga
     5161 taacgaccac cggggacgat ggagtcgtag cgggaaatag tcagtttttc gcgtaacact
     5221 tcaaccagcg cattgggcat atcgcgctga taaacaaaac gcaccggctc agcagttaaa
     5281 cgctgcttga gactggaaga catcaactcc atcaggctgg cttccatctc atgcactaaa
     5341 tcgtattcgg catcgcgggt catcttcatt gaataggcat tcagcgcgtc ataatcaaag
     5401 aagcctttga aaatatcatc gaggcagtaa cgcagaatgt tatccagaag aatcatcggc
     5461 ttgcgtcgac gtggcgcttc tggcggtaaa ttcacaaagc gcggcacttt atctgatggg
     5521 atctccagca gcgcgtaacg gatggtatcg ccacggataa tttccaccgc cagataggtg
     5581 taatcatctt tcaggaactg cactaagtca gtgtcaggat tgattaaaat cggcgtaatg
     5641 tgctgacgca gatactgctt aaaataatga cgcagccagt tttgttgatt gacggagagc
     5701 tggcgttcat taatcaggaa gatctggttg cgcgccatct ccagcaacag ctcgttgtag
     5761 aggccgtcga attcctgatc ggctttcagc acccgggact gaattttgcc cagtaaatgg
     5821 cgggaatgag agttggaacc ttgttcttcg ctaataatga tgcgtcgctt cagttcagcg
     5881 aagcggactt tatagaactc atcaaggtta ttggaataga tccccaggaa acgcatcctt
     5941 tcaatcagcg ggttagattt gtccgccgct tcctgaagca cgcgttcatt gaacgataac
     6001 caactgagct ctttttcgat gtatagcttt tcctgaccca ttaccacttt tactccgttt
     6061 tattcacggg acactgcctg gatattatgg cgagcaatat tggcagatgt ccaactatgc
     6121 cagaaaaggg ggaaaaaaac tgctggagct tgcgatttac aggtgaattg aaaaacgcca
     6181 gcggcagagc tggcgcttta actacggggc attactcgtc ggcagcgtag ccctgcggcg
     6241 gcagacgttg accatccagc cacgcggcgt tttcgtgcat tttcagacga ccatcggcaa
     6301 accagctaat caccagtgga taaatagcgt gttcctgggt ttgcacgcgg gcggtgatgt
     6361 catcttccgt atcaccagca aataccggga ctttcgcctg taaaataacc gggccaccgt
     6421 ccagttcatc ggtgacgaaa tgcaccgatg taccgtgctc ttcatcgcca ttttccagcg
     6481 cctgacgatg ggtgtgtaat ccgggatatt tcggcagcag agaagggtga atgttcagca
     6541 aacgcccggc atagtgggag acaaacgccg ggctgagaat gcgcataaaa ccagccagca
     6601 cgaccacatc gggtgcgtac atgtcgattt catgaatcaa ctcccggtca taggcttcac
     6661 gactgtcaaa cgcgctggcg atgagcgtat gcgttgcaat acccgcctgg cgggcgcgtt
     6721 caaggccgaa cgcgtcggcc ttattgctga aaactgcccg tacggtgcct ttaattttgt
     6781 tggttttgca ggcgtcaata attgcctgca aattacttcc gttgccggaa ataagcacca
     6841 caatattcat tattcgataa ccacgcgttg ttcggaatca gaggctttga tgataccgat
     6901 tttccacgcg ttttcaccgt tggcattgag cagggcgaga gctttgtcca cttccggagc
     6961 aggcagggca ataatcatac cgacgccgca gttgaaggtg cgatacattt catggcgctc
     7021 aacattacct gccgtttgca gccagttgaa cacttccggc cactgccagg aagattcatc
     7081 aatcactgcc tgggtattat ctggcaatac gcgcggaatg ttttcccaga agccgccgcc
     7141 ggtcaggtgc gcaatggcat gcacatcgac cttttcaatc aatttcagca ctgacttcac
     7201 gtaaatgcgg gtcggtgcca gcagatgatc ggctaatggc ttaccatcaa gttcggtggt
     7261 ttgcggatca caaccgctga cttcaagaat tttgcgcacc agcgaatagc cattcgagtg
     7321 cgggccgctg gagccgagtg caatcagcac atcgccgtcg ctgactttag atccgtcgat
     7381 gatttctgat ttttctacca caccaacgca gaaaccagcg acatcgtaat cttcaccgtg
     7441 atacatcccc ggcatttccg ccgtttcgcc acccaccagt gaacagcccg attgcagaca
     7501 accttccgca atgccgctga tcaccgctga agcggtatca acatccagtt ttccggttgc
     7561 gtaatagtcg aggaaaaaca gcggttccgc accttgcacc accaggtcat taacgcacat
     7621 ggcgaccaga tcaataccaa tggtgtcgtg acgttttaag tccatcgcca gacgcagctt
     7681 ggtacctacg ccgtcagtgc cggaaaccag cacaggttca cgatattttt gcggcaatgc
     7741 acacagcgca ccgaagccgc ccagaccgcc catcacttcc ggacgacgcg ttttcttcac
     7801 tacgcctttg attcttccaa ccagagcatt acccgcgtca atatcaacac cggcatcttt
     7861 gtagctaaga gaggttttat cggtcactgc ttgggtcccc acgcgttact tgcggtagaa
     7921 aaataaaatt cggcgcaatt ctaacaggga aagcaaacgt ttgcgagact gctttacaca
     7981 acctttttgc acgtcttttc cccaggcgcg cggcgaaaga atacttgtac tagggcaaag
     8041 gatcgttttc ggatggaata atcttcttcc ataaccattt gaatataaaa taactttatc
     8101 tcaaaccgtt atcattttga ctaaagtcaa cgaaaataat attgccgcct tgaagaaagg
     8161 aggtataatc cgtcgatttt ttttgtggct gcccctcaaa ggagaaagag tatgaagatc
     8221 gtggaagtca aacacccact cgtcaaacac aagctgggac tgatgcgtga gcaagatatc
     8281 agcaccaagc gctttcgcga actcgcttcc gaagtgggta gcctgctgac ttacgaagcg
     8341 accgccgacc tcgaaacgga aaaagtcact atcgaaggct ggaacggccc ggtagaaatc
     8401 gaccagatca aaggtaagaa aattaccgtt gtgccaattc tgcgtgcggg tcttgggatg
     8461 atggacggtg tgctggaaaa cgttccgagt gcgcgcatca gtgtcgtcgg tatgtaccgt
     8521 aatgaagaaa cgctggagcc ggtaccgtac ttccagaaac tggtttctaa catcgatgag
     8581 cgtatggcgc tgatcgttga cccaatgctg gcaaccggtg gttccgttat cgcgaccatc
     8641 gacctgctga aaaaagcggg ctgcagcagc atcaaagttc tggtgctggt cgctgcgcca
     8701 gaaggtatcg ctgcgctgga aaaagcgcac ccggacgtcg aactgtatac tgcatcgatt
     8761 gaccagggac tgaacgagca cggatacatt attccgggcc tcggcgatgc cggtgacaaa
     8821 atctttggta cgaaataaag aataaaaata attaaagccg actttaagag tcggcttttt
     8881 tttgagtaaa gcgcctataa cacataatac agaggataat actatgacgc gccgtgctat
     8941 cggggtgagt gaaagaccgc cacttttaca gacaatcccg cttagtttgc aacatttgtt
     9001 cgccatgttt ggtgcaaccg tcctggtgcc cgtcttattt catattaacc cggcgactgt
     9061 actgttattt aacggtatag gaacactgct gtatctcttc atctgtaaag ggaaaattcc
     9121 ggcttatctt ggttccagct ttgcctttat ttcaccggta ttgttactgt tgccgttggg
     9181 gtatgaagtt gcgctgggcg gctttattat gtgcggcgtg ctgttctgcc tggtttcttt
     9241 tatcgtgaag aaagcgggga ccggctggct ggacgtgctg tttccacctg cggcaatggg
     9301 cgcaatcgtt gccgtcatcg gtctggagct ggcgggcgta gctgccggta tggcgggttt
     9361 actcccggct gaagggcaaa cgccagactc caaaaccatc atcatctcta ttaccaccct
     9421 ggcggtcacg gttttaggtt ccgtgctgtt tcgtggtttc ctggcaatta tcccgatttt
     9481 aattggcgtg ctggtggggt acgcgctctc tttcgcaatg ggaattgtcg ataccacgcc
     9541 gattattaat gctcactggt ttgcgctgcc gaccctctat acgccgcgct tcgagtggtt
     9601 tgccattctg actattctac cagcggcgtt agtggttatt gccgaacacg tagggcacct
     9661 ggtcgtaacg gctaatatcg tcaaaaaaga tctgctgcgc gatccaggtc tgcaccgttc
     9721 gatgtttgct aacggcttgt cgaccgtgat ctccggcttc tttggctcca cgccaaacac
     9781 cacttacgga gaaaacattg gcgtgatggc gatcacccgt gtttacagta cctgggttat
     9841 cggcggtgcg gcgattttcg ctatcctgct ttcctgcgtc ggtaaactgg ctgcagctat
     9901 ccagatgatt ccattgccgg tgatgggcgg cgtttcgctg ctgctttacg gtgtcatcgg
     9961 tgcttccggt attcgtgtgt tgatcgaatc gaaagtggat tacaacaaag cacagaacct
    10021 gatcctgact tccgttattt tgatcatcgg cgtcagtggc gcgaaggtaa acattggcgc
    10081 ggcggagctg aaaggtatgg cgctggcgac catcgtcggt atcggcttaa gtttgatctt
    10141 caaactgatt agtatgctgc gccctgaaga agtggtgctg gacgcagaag acgcggatat
    10201 aacagacaaa taatgtaccg accgggcagt gttgctgccc ggttcaaaca tcatgggatt
    10261 ctgtggtaaa cttctcgcga ttttgtgaaa tcctggttga ggtatctctg aacacaccgg
    10321 cacagctctc tttgccactt tatcttcctg acgacgaaac ctttgcaagt ttctggccgg
    10381 gggataactc ctctttactg gccgcgctgc aaaacgtgct gcgtcaggaa catagcggtt
    10441 acatctatct ctgggcacgc gaaggcgcgg ggcgcagcca tctgctgcac gcggcttgcg
    10501 cggaattgtc gcagcgtggc gatgcggtgg gctatgtccc gctggataaa cgcacctggt
    10561 ttgttccgga agtgctcgac ggtatggagc atttgtcgct ggtctgtatc gacaacattg
    10621 agtgtattgc aggcgatgag ttgtgggaga tggcgatttt cgatctctac aatcgaattc
    10681 tggaatcggg caaaacacgg ttgttaatca ccggcgatcg tccaccgcgg cagttgaatc
    10741 ttggattacc ggatctcgcg tcgcgtctcg actgggggca gatctacaaa ttgcagccac
    10801 tttctgatga agataagttg caggcgctac agttacgcgc gcgtttgcgt ggttttgaac
    10861 tgccggaaga tgtggggcgt ttcttgctga agcggctcga cagagagatg cgcacgttat
    10921 ttatgacgtt ggatcagttg gatcgtgcgt cgattaccgc gcaacgtaag ctgaccattc
    10981 cgtttgtgaa agaaattctg aaactgtaga caagacgttt attgtcggat atgcacccat
    11041 agtattcgtc ggccggataa gacgcggcaa gcgtcgcatc cggcaatgtg cttcaactag
    11101 cgatggagca cttcttaacc cacgatttcc agtacctgct ccggtggacg accaatccgc
    11161 gctttgccat tcgcgaccac aatcgggcgc tccattagct tcggattatc caccattgcc
    11221 tgaatcagcg cctcttcgct aagcgaactg tcagccaggt tcagttcttt ataaagatcc
    11281 tctttctggc gcatcaattc acgagcgcta ttcatcccca gcattttcag caaatcacgc
    11341 agcgttgccg catcggcggg tgtctcaagg tagagcacca cttctggctc cacgccgttt
    11401 tctttcagca ggttcagcgt ttcccggctc tttgaacagc gtgggttatg gtagattttt
    11461 acctgtttgg tcattgttct tcctttaatg cgaattacat cttggtataa ggcttaaagc
    11521 gttcctgcag ctggcgcaac tggtcgatgc gcgcatcgta acgcgcttgt tgcaggctgc
    11581 ctaatttcac ctgcgaactg gcgctactca acagcgaaat ggcctgatcg agtcgtccgg
    11641 cgagcgcata accttctgct cgcgcagcca gctcctgatc gcggttattt agcgcggctt
    11701 ccgcctgtgc cagcaaatcc cagccgttgc tgtcatcttt attattaaag gtgtagcgat
    11761 tcagaatatt ggccgcttct tgtggttgac cgccttgcag ataagcgttc gccaggttga
    11821 gctgcaacac cggattggtg cgtaaatcgc gggcattttt cagacgattg atcgcctcat
    11881 tggctttgtt ttgcccaaga tcgatatcag tagccagatc gagataccat gcgttgccag
    11941 gttctgccgc cagtaacggt tgcagcgttt tacgcgcctc gtcgtattta ttggcttcca
    12001 tcgcctgtaa agcacgacca tattgcgccg ctcgttgctg acgaacgttt cctttcgccc
    12061 attcatccag caaatcactg gtgagctggt tacgtccgga attatacatc cccagtgtgc
    12121 gcgctttcgc cagatagaaa tcttccgacg actgcaccac catcgggcgc atctgattag
    12181 cacggttgcg ggcatctgcc agacgacttt ccggcaacgg gtgagtcaat aaaatttccg
    12241 gcggacgcga ggagtaacgc gcctgatcga gtaatttttc gaggaaggtc ggcatcgcct
    12301 gcggatcgaa tcctgagcgt tgcagcacct gaataccaat gcggtccgct tcctgttcat
    12361 tttgctgggt gaagctgatc atcccctggc gcgttcccgc cagtgtaccg gtcagcgcag
    12421 ccatccccgc ctgcggactg gccatcgcca gtaaaataga acctaacgcg ccgacccagg
    12481 tcagcggcgc gctgcgctgc tgatcttcca tcgctcgcgc caggtgacgt tgggtgacgt
    12541 gggagatttc gtgcgccata actgaagcca gttgactttc gttatcggaa taacggaaca
    12601 gggcagagtg cagcaccacg ttgccgccaa agaaagcaaa ggcgttaatt tcatcgttgt
    12661 tgatcagaaa gaaatgaaac ggtgtcttaa ccgaattggc atgcgaaacc agacgcattc
    12721 ctagcgaatt aatgtattgc gttaacagcg gatcattaat taacggcgcg ctgccgcgta
    12781 gctggcggac ataatagtcg cccatctgca tttcctgacc aatggaaagt gtgcttcctg
    12841 cggaggttcc catatccggc aaggtgtctg cgctgtcggc aaacgccggg gctacctgac
    12901 caatggtcat agcagcaatg agggttgcaa ccaggttttt tttcaactgc ctgaacataa
    12961 cctctatcct gtatttctgg agatagtcat ttgaccgata ctgtgaaata tcgttcccac
    13021 cgcgccgcct ttgagtgtaa cggcgaagat tagcccactc aaatgacttt tcagtttcct
    13081 gaatccgtca taaaaagcga agtctttttt gtggcatttc gatacaattg cggatcgcaa
    13141 tcccgctctt gaggttcagg gaaggtctgt atgctcgaaa tgttgatgca atggtatcgc
    13201 cgccgtttta gcgacccgga agcgattgcc ttgctggtta ttttagttgc cggatttggc
    13261 attatctttt tctttagtgg cctgcttgct ccgctgttgg tggctattgt gctggcctat
    13321 ttgctggaat ggccaaccgt gcgcctgcaa tctattggct gctcccgccg ctgggcgacg
    13381 tcgattgtat tggtggtttt cgtcggtata ttgctactga tggcgttcgt ggtactgcct
    13441 atcgcctggc agcagggcat ctacttaatc cgcgatatgc cggggatgct caataagctt
    13501 tctgactttg ccgccacgtt gccgcgccgc tatccggcgt taatggatgc gggcattatt
    13561 gatgcaatgg ccgaaaatat gcgcagtcgg atgctgacca tgggcgattc ggtggtgaaa
    13621 atttccctcg cctcgctggt cggtttgctg accattgccg tctatctggt gctggtacca
    13681 ttgatggtct tcttcctgct gaaagacaaa gagcagatgc tgaacgccgt tcgccgggtg
    13741 ctgccgcgta accgtggact ggcaggacag gtgtggaagg agatgaatca acaaatcacc
    13801 aactatatcc gcggcaaagt gctggagatg atcgtggtgg ggattgccac ctggctggga
    13861 ttcttactct tcgggctgaa ctattcgctg ctgctggcgg tgctggtcgg cttctcggta
    13921 cttattccgt acattggtgc atttgtggtg accattccgg tggttggcgt ggcgctattc
    13981 cagtttggtg ctggcacgga attctggagc tgcttcgcgg tgtatctgat cattcaggcg
    14041 ctggacggca acctgttagt acctgtgttg ttctccgaag cggttaacct gcatccgctg
    14101 gtcattattt tatcagtagt gatcttcggt ggtttgtggg gattctgggg cgtattcttc
    14161 gccattccat tggcgacgct gatcaaagcc gtgattcacg cctggcccga tgggcaaatc
    14221 gcgcaagact gaccttcagc gaaaaaattt gaaacgagtg ttgcagggca gtgagaagat
    14281 gtgagatgtg ctggccgcaa gcgcagccag cacggaatgg agcaaagtaa tcaggcgtgt
    14341 tctttcagcc agttcagcac aacgtcgtgg tgattgctgg ttttgaaatc gtcaaagaca
    14401 tgttcgattt tgccatcagc gtcaatcagg aagctgatgc gatgaatacc atcgtaggtt
    14461 ttgcccatga aggacttttc accccagacg ccgaattgtt cgcacacctg gtggtcctca
    14521 tcagacagga gcgtaaagtt aagcagctct ttttccgcaa aacgggagag tttttcgggt
    14581 ttatcggtgc tgatacccag cacatcaacg cccgcttttt tcaactcatc catgttatcg
    14641 cgtaagccgc aggcctgtac ggtacagccg ggggtcatgg ctttcgggta gaaataaacc
    14701 agaacacgct gtccctggaa gtcggtcaaa ttaacttgtt ctccgtcttg atccggcaag
    14761 ctaaatttcg gtgcgatatc accggctttc agtggattca ttacttaact ccatcctgtt
    14821 catcatgttg ggaataattg acgacgttaa tactgccttg tgcattgagt tctgtacata
    14881 gggctttgaa tgcttgctca atatttgctg cgtccgcaga tgcggggctg tgggcggtta
    14941 tctgaatatg caactgcgca gcccgttcat tttcagcagg ttgcgtgcgc gacaccagct
    15001 ccgcaatgtt catatgatgc gcgtcgaaaa gtgctgtgaa gcgttcaatt aaatgcgggg
    15061 agtctgccac atcgacctga acccagacag atgctggcat tggcggacgc ggacgcgccg
    15121 tcgtgcgctt catcacgatt aaaagatcca gctcggcacc tttcaacggt aacgttgatt
    15181 caatcagagt aatggcattc catgaaccgg aaagcagcat gataaacgtg aactcttctc
    15241 ccagcatcgc caggcgactg tcttcaatat tacagccgca actactgaca tgacgggtga
    15301 tggtgttcac aattccaggg cgatcggcac ccaacgcagt gatcaccaga taatgttgcg
    15361 atgacagtgt caaactggtt attccttcaa ggggtgagtt gttcttaagg aaagcataaa
    15421 aaaaacatgc atacaacaat cagaacggtt ctgtctgctt gcttttaatg ccataccaaa
    15481 cgtaccattg agacacttgt ttgcacagag gatggcccat gttcacggga agtattgtcg
    15541 cgattgttac tccgatggat gaaaaaggta atgtctgtcg ggctagcatg aaaaaactga
    15601 ttgattatca tgtcgccagc ggtacttcgg cgatcgtttc tgttggcacc actggcgagt
    15661 ccgctacctt aaatcatgac gaacatgctg atgtggtgat gatgacgctg gagctggctg
    15721 acgggcgcat tccggtgatt gccgggaccg gtgctaacgc tactgcggaa gccattagcc
    15781 tgacgcagcg cttcaatgac agcggtatcg tcggctgcct gacggtaacc ccttactaca
    15841 atcgtccgtc gcaagaaggt ttgtatcagc atttcaaagc catcgctgaa catactgacc
    15901 tgccgcaaat tctgtataat gtgccgtccc gtactggctg cgatctgctc ccggaaacgg
    15961 tgggccgtct ggcgaaagta aaaaatatta tcggaatcaa agaggcaaca gggaacttaa
    16021 cgcgtgtaaa ccagatcaaa gagctggttt cagatgattt tgttctgctg agcggcgatg
    16081 atgcgagcgc gctggacttc atgcaattag gcggtcatgg ggttatttcc gttacggcta
    16141 acgtcgcagc gcgtgatatg gcccagatgt gcaaactggc agcagaaggg cattttgccg
    16201 aggcacgcgt tattaatcag cgtctgatgc cattacacaa caaactattt gtcgaaccca
    16261 atccaatccc ggtgaaatgg gcatgtaagg aactgggtct tgtggcgacc gatacgctgc
    16321 gcctgccaat gacaccaatc accgacagtg gtcgtgagac ggtcagagcg gcgcttaagc
    16381 atgccggttt gctgtaaagt ttagggagat ttgatggctt actctgttca aaagtcgcgc
    16441 ctggcaaagg ttgcgggtgt ttcgcttgtt ttattactcg ctgcctgtag ttctgactca
    16501 cgctataagc gtcaggtcag tggtgatgaa gcctacctgg aagcggcacc gcttgcggag
    16561 cttcatgccc cggctggaat gattttgccg gtgacctccg gcgattatgc aatcccggtg
    16621 accaacggta gtggtgctgt cggtaaggcg ctggacattc gtccaccagc ccagccgttg
    16681 gcactggttt ctggcgcgcg tacccagttc acgggcgata ccgcttcatt gctggtggaa
    16741 aatggtcgtg gcaatactct gtggccgcag gtggttagcg tgctgcaggc gaaaaactac
    16801 accatcaccc aacgtgatga tgctggtcag acactgacca ccgattgggt acaatggaac
    16861 cgtctggacg aagacgagca gtatcgtggt cgttatcaaa tctctgttaa gccgcagggt
    16921 tatcagcagg cggttacggt taaactgctg aacctggaac aggcgggcaa accggttgca
    16981 gacgcggctt ccatgcagcg ttacagcacg gagatgatga acgttatttc cgccggtctg
    17041 gataaatctg ccactgacgc cgcgaacgct gcgcaaaatc gtgcctccac cactatggac
    17101 gtacaaagtg cagctgatga caccggttta ccaatgctgg tcgtacgcgg gccgttcaat
    17161 gtggtttggc aacgtctgcc agctgcgctg gaaaaagtgg gcatgaaagt gaccgacagc
    17221 acccgttcgc agggcaacat ggccgtaact tacaagccgc tgtctgacag cgaatggcac
    17281 gaactgggcg cgagcgatcc aggtctggca tccggtgact ataaactgca ggttggcgat
    17341 ttagataacc gcagcagcct acagttcatc gatccgaaag gtcatactct gactcagagt
    17401 cagaacgacg cgctggtagc tgtcttccag gctgcgttta gcaagtaaaa atacagggct
    17461 ggagtcatcc ggcccttttt tctgatatga tacgcaaacg tgtgcgtcgg cagaaaaacg
    17521 cgattttagc ggtaattcgc acgaaatttg tttgtcggac gtaggtcgga taaggcgttc
    17581 acgccgcatc cgacaaaaca tccggcacac cagacagcaa tagattttaa aacgttaatt
    17641 cacacccagg agtgataaag atgcaaaagc aagctgagtt gtatcgtggt aaagcgaaaa
    17701 ccgtatacag cacggaaaac ccggacctgt tggtgctcga attccgcaat gatacgtcag
    17761 caggggatgg cgcgcgcatt gagcagtttg atcgcaaagg tatggtgaac aacaagttca
    17821 actacttcat tatgagcaaa ctggctgaag cgggtatccc gactcaaatg gagcgtctgc
    17881 tctccgatac cgaatgtctg gtgaaaaagc tggatatggt gccggttgag tgtgtcgtgc
    17941 gtaaccgtgc tgctggctct ctggtgaaac gtcttggaat cgaagaaggt attgagctga
    18001 acccgccact gttcgatctg ttcctgaaaa acgacgccat gcacgatccg atggtcaacg
    18061 aatcttactg cgaaaccttt ggctgggtaa gcaaagagaa cctggcacgc atgaaagagc
    18121 tgacctacaa agcgaacgac gtgctgaaaa aactgttcga tgatgctggt ctcattctgg
    18181 tcgacttcaa gctggagttt ggtctgtaca aaggtgaagt ggtactgggc gatgagttct
    18241 ccccggacgg cagccgcttg tgggacaaag aaacgctgga gaaaatggac aaagaccgtt
    18301 tccgccagag cctcggtggc ctgatcgagg cctatgaagc cgtcgcccgc cgcctgggtg
    18361 tacagctgga ctgatttttc tgttcatcat cttgccgtgc agctggcacg gcaagacaac
    18421 cgctctcata agatgagtat tgaacgctca ttcctccgcc atttcatccc gctttcctgt
    18481 ggtaatcttc ctctgaacca cctacgatca tttctataat gaatatattg ttgaggtatc
    18541 tatgcgttgg caagggcgac gtgaaagtga caatgttgaa gacaggcgca acagctctgg
    18601 tggtccgtca atgggcggtc caggttttcg cctgccaagc ggtaaaggcg ggctgatttt
    18661 actgatagtc gtgctggttg caggctacta tggcgttgat ttaaccgggt tgatgaccgg
    18721 acagccggtt tcccaacagc aatcaacacg gtcaattagt cctaatgaag acgaagccgc
    18781 aaaattcact tcggtgattc tggcaaccac ggaagacacc tggggacagc aattcgagaa
    18841 gatgggtaaa acctatcagc aaccgaagct ggtcatgtac cgtggaatga cgcgcaccgg
    18901 ctgcggggcg ggccagtcca taatgggacc gttctattgc ccggcggatg gcacggttta
    18961 tatcgatctc tccttctatg atgacatgaa agacaaactt ggcgcggatg gcgattttgc
    19021 ccaggggtac gttatcgccc atgaagtcgg tcatcatgtg cagaaactgt taggcatcga
    19081 gccgaaagtt cgtcaactgc aacaaaacgc gacgcaggcg gaagtgaacc gcttatctgt
    19141 gcgtatggaa ctccaggccg actgttttgc cggtgtctgg gggcatagta tgcagcagca
    19201 aggtgttctg gaaaccggcg atctggaaga ggcgctgaac gcggcgcagg ccatcggcga
    19261 tgaccgttta caacagcaaa gtcaggggcg agtagtacca gacagtttca ctcatggcac
    19321 ttctcagcaa cgctacagct ggtttaaacg tggtttcgac agcggcgatc cggcacaatg
    19381 caatactttt ggtaaaagca tttaacttcc ggggcaggga tggctgaact gactgcgctt
    19441 cacacattaa cagcgcaaat gaaacgtgaa gggatccgcc gcttgctggt gttgagcggg
    19501 gaagagggtt ggtgttttga tcatgcgctt aagttgcgtg atgccttacc tggcgactgg
    19561 ctgtggattt cgccgcagcc agatgctgaa aaccactgtt ctccctcggc gctacaaact
    19621 ttacttgggc gcgagttccg gcatgcggta ttcgacgccc gccacggctt tgatgccgct
    19681 gcctttgcgg cacttagcgg aacgttgaaa gcgggaagct ggctggtttt gttactccct
    19741 gtatgggaag agtgggaaaa ccaacctgat gccgactcgc tgcgatggag tgattgccct
    19801 gaccctattg cgacgccgca ttttgtccag catctcaaac gcgtacttac ggcgaataac
    19861 gacgctatcc tctggcggca gaaccagccg ttcacgttgg cgcattttac tccccgtact
    19921 gactggcacc ccgcgaccgg cgcaccacag ccagaacaac agcaactctt acagcagcta
    19981 ctgaccatgc cattgggcgt ggcggtggta acggctgcgc gtgggcgcgg taaatcggcg
    20041 ctggcagggc aactcatttc tcgtattgcg ggtagtgcga ttgtcaccgc gcccgcaaaa
    20101 gcggcaacgt atgtactggc acaatttgcg ggcgagaagt ttcgctttat tgcgccggat
    20161 gccttgttag ccagcgatga gcaagccgac tggctggtgg tcgatgaagc cgcagccata
    20221 cctgcgccgt tgttgcatca actggtatcg cgttttcctc gaacgttgtt aaccactacg
    20281 gtgcagggct acgaaggcac gggacgtggt tttttgctga aattttgcgc tcactttccg
    20341 catttacacc gttttgaact gcaacagccg atccgctggg cacagggatg cccgctggaa
    20401 aaaatggtta gtgaggcact ggtttttgac gatgaaaact tcacccacac accacaaggc
    20461 aatatcgtca tttccgcatt tgaacagacg ttatggcgaa gcgagccaca aacgccgtta
    20521 aaggtttatc aattattgtc tggtgcgcac taccggactt cgccgctgga tttacgccgc
    20581 atgatggatg caccagggca acatttttta caggcggctg gcgaaaacgc gattgccggg
    20641 gcgctgtggc tggtggatga gggcggatta tctcaagaac tcagtcaggc ggtatgggca
    20701 ggttttcgtc gcccgcgggg taatctggtg gcccagtcgc tggcggcgca cggcagcaat
    20761 ccactggcgg cgacattgcg cggaaggcgg gttagccgga tagcagttca tccggctcgt
    20821 cagcgggaag gcacaggtca gcaactgatt gctggtgctt tgcaatatac gcatgacctc
    20881 gactatcttt cggtgagttt tggttacacc ggggaattat ggcgtttctg gcaacgctgc
    20941 ggttttgtgc tggtgcgaat gggtaatcat cgtgaagcca gcagcggttg ctatacggcg
    21001 atggcgctgt taccgatgag tgatgcgggt aaacagctgg ctgaacgtga gcattaccgt
    21061 ttacgtcgcg atgcgcaagc tctcgcaaag tggaatggcg aaacgctccc tgttgatcca
    21121 ctaaacgata tcgtgctttc tgccgacgac tggcttgaac tggccggttt tgctttcgct
    21181 catcgtccgc tattaacgtc gttaggttgc ttaatgcgtc tgttacaaac cagcgaaatg
    21241 gcattaccgg cgctgcgtgg gcgtttacag aaaaacgcca gcgacgcgca gttatgtacc
    21301 acacttaaac tttcaggccg taagctgtta ctggtccgtc agcgggaaga ggccgcgcag
    21361 gcgctatacg cacttgatga tgttcgcact gagcgtttgc gcgatcgcat aacgcaatgg
    21421 caattttttc actgactcct tcaattttct cccatggtca ttgtgagtca gcggcgtaaa
    21481 attaatgaca tcagttaagg agttcgccat gaaacatgac cattttgttg ttcaaagccc
    21541 ggataaacct gcgcaacagt tgttactgct tttccacggt gtcggggata acccggtggc
    21601 aatgggggaa ataggtagct ggtttgcgcc gctatttcct gatgcgctgg tggtgagtgt
    21661 cggaggcgcg gagccgagcg gtaatccggc ggggcgtcag tggttttcgg tgcagggtat
    21721 tacggaagat aatcgccagg cgcgtgtgga tgccatcatg ccgacgttta ttgagacggt
    21781 gcgctactgg cagaaacaga gcggagtagg ggctaccgcc acggcgttaa tcggtttctc
    21841 tcaaggcgcg attatggcgc tggagagcat taaagccgaa ccgggccttg cctcacgcgt
    21901 cattgccttt aacggacgct atgcgagcct gccggaaact gcgtctacag ccacgacaat
    21961 tcacctgatt catggcggtg aagatccggt tatcgatctg gcgcatgcgg ttgcggcgca
    22021 ggaagcgtta atcagtgccg gtggtgacgt gacgctggat atcgtggagg atttaggcca
    22081 tgcgatcgac aatcgcagta tgcaatttgc tctggatcac ttgcgttata ccattccgaa
    22141 acattatttc gacgaagcgc taagcggcgg taagcctggc gatgatgatg tgattgaaat
    22201 gatgtaataa tagccgggta ggtctgataa gactgtatca ggcatcatgg ccggatgcgg
    22261 cgttaacgcc ttatccggcc acttagctta cttcttcggt tgatcctttt tcggccagtc
    22321 gtcgtcatcg tcccacttat cgttgaaatc acgatgcggg ggaagctccg gcttgttggc
    22381 gagaaatttc ttatggttga cgcgcttgag atctttaatc acgttcagca ggacgcctac
    22441 caaaaagaca atcaccagaa tccaccaata tttagccagc cagtccatgc ttatttcctc
    22501 ttgcagaacc actcatcagg cgacgagctg ttccatgata cgttgataca tacgggcaag
    22561 tagctgcagg tcggcagcgt tcacacattc attaatttta tgaatagtgg cattgaccgg
    22621 cccgagttcc accacctgcg cccccatgcg ggcaataaag cgcccgtcgg acgttccgcc
    22681 tgtggttagt agctgcggtt taatttcatt atagtgctca accgcgttaa cgaccgcatc
    22741 caccagttta ccgcgcgcgg tcaaaaatgg ctgcccggaa agccaccaat ccaccgtata
    22801 gcgcagttga tgtttttcaa gcagggcaag tacctgcgct ttgatcatct catcagtcag
    22861 ttcggtgctg aagcggaagt taaactgcac aaacagttca cccggaataa cgttgttact
    22921 gcccgttccc gcctgaatat tggcaatctg catactggtc gccgggaaga attcattgcc
    22981 ctgatcccac tcaatagcca ctaattcatt aaggaaaggt gctgcgcgat gtaccggatt
    23041 gtcagccaga tgtgggtagg caacatgccc ctgcacgcca tgaatggtga ggttgcaggt
    23101 taacgatccg cgacgaccat ttttcaccac atcaccaacc acttcgatac tcgacggttc
    23161 gccaaccaga cagtaatcga gacgctcatt acgcgccatt aacgcttcga cgacttttac
    23221 cgtaccgttg tgggcactag cttcttcatc agaggtgatc agaaatgcca gtcgccccgt
    23281 atggttggga tgttgtgcga caaaacgttc tgccgctacc accatcgccg ccagcgagcc
    23341 tttcatatct gccgcaccgc gcccgaataa catgccgtca cgaatggtgg gttcaaacgg
    23401 cggattgatc caacggtcgg cgtcgccagg cggcaccacg tcggtatgcc cggcaaaggc
    23461 taacgtttca ccctgcccac gccatgccca aaaattctgc gtatcggcaa agtccatgcg
    23521 ttcaacggta aaaccgatcg cctgcaaacg ttcaatcaac aaagcctggc atcctgcgtc
    23581 atcaggactc agggaagggc ggcgaataag ctgttgtgtc agctcaataa ccgggcacga
    23641 catagactac acctcatgga aaaattgctg ataactggat tcactgaaac ccagcagcat
    23701 aggcttacca ggcgcgcaga gcaatggacg tttgataatt gcaggcattt cagtcattaa
    23761 tgtcgccgca gaggccgcat cggtgatttt attgcgggtg gtttcgtcca gtttacgcca
    23821 ggttgtacca cgggtgttga gtaacgcttc ccagcctaat tcgttgataa aaccgttcaa
    23881 taattcgctg tccaatccat cgacgcggta atcatgaaag cgatagtcga tgttattggc
    23941 ttccagccaa cggcgagcct ttttaatggt gtcacaattt ttgatgccgt aaagtgtaac
    24001 cataatgaat c
//
LOCUS       DAZFLD010000062        23898 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.062, whole genome shotgun sequence.
ACCESSION   DAZFLD010000062 DAZFLD010000000
VERSION     DAZFLD010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23898)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23898)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23898
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.062"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(225..1874)
                     /gene="pgi"
                     /locus_tag="ACHKBL_002063"
     CDS             complement(225..1874)
                     /gene="pgi"
                     /locus_tag="ACHKBL_002063"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HFO5818940.1"
                     /translation="MKNINPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSAT
                     FDDQMLVDYSKNRITEETLAKLQDLAKECDLAGAIKSMFSGEKINRTENRAVLHVALR
                     NRSNTPILVDGKDVMPEVNAVLEKMKTFSEAIISGEWKGYTGKAITDVVNIGIGGSDL
                     GPYMVTEALRPYKNHLNMHFVSNVDGTHIAEVLKKVNPETTLFLVASKTFTTQETMTN
                     AHSARDWFLKAAGDEKHVAKHFAALSTNAKAVGEFGIDTANMFEFWDWVGGRYSLWSA
                     IGLSIVLSIGFDNFVELLSGAHAMDKHFSTTPAEKNLPVLLALIGIWYNNFFGAETEA
                     ILPYDQYMHRFAAYFQQGNMESNGKYVDRNGKVVDYQTGPIIWGEPGTNGQHAFYQLI
                     HQGTKMVPCDFIAPAITHNPLSDHHQKLLSNFFAQTEALAFGKSREVVEQEYRDQGKD
                     PATLDYVVPFKVFEGNRPTNSILLREITPFSLGALIALYEHKIFTQGVILNIFTFDQW
                     GVELGKQLANRILPELKDDKEISSHDSSTNGLINRYKAWRG"
     regulatory      2111..2307
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            2399..3748
                     /gene="lysC"
                     /locus_tag="ACHKBL_002064"
     CDS             2399..3748
                     /gene="lysC"
                     /locus_tag="ACHKBL_002064"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HFO5818941.1"
                     /translation="MSEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGI
                     TNLLVALAEGLEPGERFEKLDAIRNIQFAILERLRYPNVIREEIERLLENITVLAEAA
                     ALATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKVMRTNDRFGRAEPDVA
                     ALAELAALQLLPRLNEGLVITQGFIGSENKGRTTTLGRGGSDYTAALLAEALHASRVD
                     IWTDVPGIYTTDPRVVSAAKRIDEIAFAEAAEMATFGAKVLHPATLLPAVRSDIPVFV
                     GSSKDPRAGGTLVCNKTENPPLFRALALRRNQTLLTLHSLNMLHSRGFLAEVFGILAR
                     HNISVDLITTSEVSVALTLDTTGSTSTGDTLLTQSLLMELSALCRVEVEEGLALVALI
                     GNDLSKACGVGKEVFGVLEPFNIRMICYGASSHNLCFLVPGEDAEQVVQKLHFNLFE"
     gene            4001..4273
                     /locus_tag="ACHKBL_002065"
     CDS             4001..4273
                     /locus_tag="ACHKBL_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3811 domain-containing protein"
                     /protein_id="HFO5818942.1"
                     /translation="MALPRITQKEMTEREQRELKTLLDRARIAHGRVLTNSETNSIKK
                     EYIDKLMVEREAEAKKARQLKKKQAYKPDPEASFSWSANTSTRGRR"
     gene            complement(4274..5146)
                     /gene="rluF"
                     /locus_tag="ACHKBL_002066"
     CDS             complement(4274..5146)
                     /gene="rluF"
                     /locus_tag="ACHKBL_002066"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HFO5818943.1"
                     /translation="MLPDSSVRLNKYISESGICSRREADRYIEQGNVFLNGKRATIGD
                     QVKPGDVVKVNGQLIEPREAEDLVLIALNKPVGIVSTTEDGERDNIVDFVNHSKRVFP
                     IGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPIL
                     GTVTKKCKVKKEAPFVFRITLVQGLNRQIRRMCEHFGYEVKKLERTRIMNVSLSGIPL
                     GEWRDLTDDELIDLFKLIENSSSEAKPKAKAKPKTVGIKRPVVKMEKTAEKGGRPASN
                     GKRFTSPGRKKKGR"
     gene            5494..6441
                     /locus_tag="ACHKBL_002067"
     CDS             5494..6441
                     /locus_tag="ACHKBL_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HFO5818944.1"
                     /translation="MENSDDIRLIVKIAQLYYEQDMTQAQIARELGIYRTTISRLLKR
                     GRDQGIVTIAINYDYNENLWLEQQLKQKFGLKDVVVVSGNDEDEETQLAMMGLHGAQL
                     LDRLLEPGDIVGFSWGRAVNALVENLPQAGQSRQLICVPIIGGPSGKLESRYHVNTLT
                     YSAAAKLKGESHLADFPALLDNPLIRNGIMQSQHFKTISAYWDNLDVALVGIGSPAIR
                     DGANWHAFYGGEESDDLNARQVAGDICSRFFDIHGAMVETNMSEKTLSIEMNKLKQAR
                     YSIGIAMSEEKYSGIIGALRGKYINCLVTNSSTAELLLK"
     gene            6511..7317
                     /locus_tag="ACHKBL_002068"
     CDS             6511..7317
                     /locus_tag="ACHKBL_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFO5818945.1"
                     /translation="MQTWLNLQDKIIIVTGGASGIGLAIVDELLAQGANVQMVDIHGG
                     DGQYEGHKGYQFWPTDISSAKEVNHTVAEIIQRFGRIDGLVNNAGVNFPRLLVDEKAP
                     AGQYELNEAAFEKMVNINQKGVFLMSQAVARQMVKQHDGVIVNVSSESGLEGSEGQSC
                     YAATKAALNSFTRSWSKELGKHGIRVVGIAPGILEKTRLRTPEYEEALAWTRNITVEQ
                     LREGYTKNAIPIGRAGRLAEVTDFVCYLLSQRASYITGVTTNIAGGKTRG"
     gene            7327..7734
                     /locus_tag="ACHKBL_002069"
     CDS             7327..7734
                     /locus_tag="ACHKBL_002069"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIA"
                     /protein_id="HFO5818946.1"
                     /translation="MVNAIFCAHGKLACAMLESVQMVYGNANVEAVEFVPGENAGDIV
                     AKLEKLVSIHNQDEWLIAVDLQCGSPWNAAAMLAMRNPRLRVISGLSLPLALELVDNQ
                     HSMNVDELCEHLTQIAKQTCVVWKQLATAEEDF"
     gene            7734..8228
                     /locus_tag="ACHKBL_002070"
     CDS             7734..8228
                     /locus_tag="ACHKBL_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFO5818947.1"
                     /translation="MNITLARIDDRLIHGQVTTVWSKVANAQHIIICNDEVYNDEVRR
                     TLLRQAAPPGMKVNVVNIEKAVAVYHNPQYQDETVFYLFTRPQDALAMVRQGVKIDTL
                     NIGGMAWRPGKKQLTKAVSLDDDDINAFHELNNLGVILDLRVVASDPSINIIDKINEQ
                     LIAN"
     gene            8294..9091
                     /locus_tag="ACHKBL_002071"
     CDS             8294..9091
                     /locus_tag="ACHKBL_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HFO5818948.1"
                     /translation="MEISTLQIIAIFLFSCIAGMGSVLDEFQTHRPLIACTVIGLILG
                     DLKTGIMLGGTLELIALGWMNVGAAQSPDSALASIISAILVIVGQQSIATGIAIALPV
                     AAAGQVLTVFARTITVVFQHAADKAAEEARFRTLDILHVSALGVQALRVAIPALIVSL
                     FVSADMVSNMLSAIPEFVTRGLQIAGGFIVVVGYAMVLRMMGVKYLMPFFFLGFLAGG
                     YLDLSLLAFGGVGVIMALLYIQLNPQWRKAEPQLQATASTALDQLDD"
     gene            9102..9926
                     /locus_tag="ACHKBL_002072"
     CDS             9102..9926
                     /locus_tag="ACHKBL_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFO5818949.1"
                     /translation="MEQRKITRSDLVSMFLRSNLQQASFNFERIHGLGFCYDMIPAIK
                     RLYPLKEDQVAALRRHLVFFNTTPAVCGPVIGVTAAMEEARANGAEIDDGTINGIKVG
                     LMGPLAGVGDPLVWGTLRPITAALGASLALSGNILGPLLFFFIFNAVRLAMKWYGLQL
                     GFRKGVNIVSDMGGNVLQKLTEGASILGLFVMGVLVTKWTSINVPLLVSQTHAADGST
                     VTMTVQNILDQLCPGLLALGLTLLMVRLLNKKINPVWLIFALFGLGIIGNALGFLS"
     gene            9978..11207
                     /gene="sorE"
                     /locus_tag="ACHKBL_002073"
     CDS             9978..11207
                     /gene="sorE"
                     /locus_tag="ACHKBL_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-sorbose 1-phosphate reductase"
                     /protein_id="HFO5818950.1"
                     /translation="MKTTALRLYGKRDLRLETFDLPEMQEDEILATVVTDSLCLSSWK
                     EANLGENHKKVPDDVATNPIIIGHEFCGDILAVGKKWQHKFQPGQRYVIQANLQLPDR
                     PDCPGYSFPWVGGEATHVVIPNEVMEQDCLLAYDGETYFEGSLVEPLSCVIGAFNANY
                     HLQEGSYNHTMGIRPQGRTLILGGTGPMGLLAIDYALHGPVNPSLLVITDTDNDKLSY
                     ARKHYPSEPQTLIHYLNAADAAFDTLMALSGGHGFDDIFVFVPNEGLVTLASSLLATD
                     GCLNFFAGPQDKHFSAPINFYDVHYAFTHYVGTSGGNTDDMRAAVKLIEEKKVQAAKV
                     VTHILGLNAAGETTLELPAIGGGKKLVYTGKYLPLMSLTQIQDQALAAILARHQGIWS
                     GEAEQYLLAHAEAISHD"
     gene            11589..12278
                     /gene="pepE"
                     /locus_tag="ACHKBL_002074"
     CDS             11589..12278
                     /gene="pepE"
                     /locus_tag="ACHKBL_002074"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HFO5818951.1"
                     /translation="MELLLLSNSTLPGKAWLEHALPLIAEQLQGRRSAVFIPFAGVTQ
                     TWDDYTAKTAAVLAPLGVSVTGIHSVVDPVAAIENAEIVIVGGGNTFQLLKQCRERGL
                     LAPITDVVKRGALYIGWSAGANLACPTIRTTNDMPIVDPQGFDALNLFPLQINPHFTN
                     ALPEGHKGETREQRIRELLVVAPELTIIGLPEGNWITVSKGHATLGGPNTTYVFKAGE
                     EAVPLEAGHRF"
     gene            complement(12368..13999)
                     /locus_tag="ACHKBL_002075"
     CDS             complement(12368..13999)
                     /locus_tag="ACHKBL_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HFO5818952.1"
                     /translation="MLTLLHLLSAVALLVWGTHIVRTGVMRVFGARLRTVLSRSVEKK
                     PLAFCAGIGVTALVQSSNATTMLVTSFVAQDLVALAPALVIVLGADVGTALMARILTF
                     DLSWLSPLLIFIGVIFFLGRKQSRAGQLGRVGIGLGLILLALELIVQAVTPITQANGV
                     QVIFASLTGDILLDALIGAMFAIISYSSLAAVLLTATLTAAGIISFPVALCLVIGANL
                     GSGLLAMLNNSAANAAARRVALGSLLFKLVGSLIILPFVHLLAETMGKLPLPKAELVI
                     YFHVFYNLVRCLVMLPFVDPMARFCKTIIRDEPELDTQLRPKHLDVSALDTPTLALAN
                     AARETLRIGDAMEQMMEGLNKVMHGEPRQEKELRKLADDINVLYTAIKLYLARMPKEE
                     LAEEESRRWAEIIEMSLNLEQASDIVERMGSEIADKSLAARRAFSLDGLKELDALYEQ
                     LLSNLKLAMSVFFSGDVTSARRLRRSKHRFRILNRRYSHAHVDRLHQQNVQSIETSSL
                     HLGLLGDMQRLNSLFCSVAYSVLEQPDEDEGRDEY"
     gene            complement(14219..17902)
                     /gene="metH"
                     /locus_tag="ACHKBL_002076"
     CDS             complement(14219..17902)
                     /gene="metH"
                     /locus_tag="ACHKBL_002076"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HFO5818953.1"
                     /translation="MSSKVEQLRAQLNERILVLDGGMGTMIQSYRLNEADFRGERFAD
                     WPCDLKGNNDLLVLSKPEVIAAIHNAYFEAGADIIETNTFNSTTIAMADYQMEFLSAE
                     INFAAAKLARACADEWTARTPEKPRYVAGVLGPTNRTASISPDVNDPAFRNITFDGLV
                     AAYRESTKALVEGGADLILIETVFDTLNAKAAVFAVKTEFEALGVELPIMISGTITDA
                     SGRTLSGQTTEAFYNSLRHAEALTFGLNCALGPDELRQYVQELSRIAECYVTAHPNAG
                     LPNAFGEYDLDADTMAKQIREWAQAGFLNIVGGCCGTTPQHIAAMSRAVEGLAPRKLP
                     EIPVACRLSGLEPLNIGEDSLFVNVGERTNVTGSAKFKRLIKEEKYSEALDVARQQVE
                     NGAQIIDINMDEGMLDAEAAMVRFLNLIAGEPDIARVPIMIDSSKWDVIEKGLKCIQG
                     KGIVNSISMKEGVDAFIHHAKLLRRYGAAVVVMAFDEQGQADTRARKIEICRRAYKIL
                     TEEVGFPAEDIIFDPNIFAVATGIEEHNNYAQDFIGACEDIKRELPHALISGGVSNVS
                     FSFRGNDPVREAIHAVFLYYAIRNGMDMGIVNAGQLAIYDDLPTELRDAVEDVILNRR
                     DDGTERLLELAEKYRGSKTDDTANTQQAEWRSWEVNKRLEYSLVKGITEFIEQDTEEA
                     RQQATRPIEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAYLEPFIEASK
                     EQGKTNGKMVIATVKGDVHDIGKNIVGVVLQCNNYEIVDLGVMVPAEKILRTAKEVNA
                     DLIGLSGLITPSLDEMVNVAKEMERQGFTIPLLIGGATTSKAHTAVKIEQNYSGPTVY
                     VQNASRTVGVVAALLSDTQRDDFVARTRKEYETVRIQHGRKKPRTPPVTLEAARDNDF
                     AFDWQAYTPPVAHRLGVQEVEASIETLRNYIDWTPFFMTWSLSGKYPRILEDEVVGVE
                     AQRLFKDANDMLDKLSAEKTLNPRGVVGLFPANRVGDDIEIYRDETRTHVINVSHHLR
                     QQTEKTGFANYCLADFVAPKLSGKADYIGAFAVTGGLEEDALADAFEAQHDDYNKIMV
                     KALADRLAEAFAEYLHERVRKVYWGYAPNENLSNEELIRENYQGIRPAPGYPACPEHT
                     EKATIWELLEVEKHTGMKLTESFAMWPGASVSGWYFSHPDSKYYAVAQIQRDQVEDYA
                     RRKGMSVSDVERWLAPNLGYDAD"
     gene            18102..18926
                     /gene="iclR"
                     /locus_tag="ACHKBL_002077"
     CDS             18102..18926
                     /gene="iclR"
                     /locus_tag="ACHKBL_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate bypass operon transcriptional
                     repressor IclR"
                     /protein_id="HFO5818954.1"
                     /translation="MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSV
                     ALTELAQQAGLPNSTTHRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLL
                     AIVHPILRNLMEESGETVNMAVLDQSNHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
                     AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHAL
                     GLRCLAACIFDEHREPFAAISISGPISRITDDRVTEFGAMVIKAAKEVTLAYGGIR"
     gene            complement(18943..20667)
                     /gene="aceK"
                     /locus_tag="ACHKBL_002078"
     CDS             complement(18943..20667)
                     /gene="aceK"
                     /locus_tag="ACHKBL_002078"
                     /EC_number="2.7.11.5"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008772 - [isocitrate dehydrogenase
                     (NADP+)] kinase activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
                     /protein_id="HFO5818955.1"
                     /translation="MPRGLELLIAQTILQGFDAQYGRFLEVTSGAQQRFEQADWHAVQ
                     QAMKNRIHLYDHHVGLVVEQLRCITNGQSTDAAFLLRVKEHYTRLLPDYPRFEIAESF
                     FNSVYCRLFDHRSLTPERLFIFSSQPERRFRTIPRPLAKDFHPDHGWESLLMRVISDL
                     PLRLRWQNKSRDIHYIVRHLTETLGTDNLAESHLQVANELFYRNKAAWLVGNLITPSG
                     TLPFLLPIHQTDDGELFIDTCLTTTAEASIVFGFARSYFMVYAPLPAALVEWLREILP
                     GKTTAELYMAIGCQKHAKTESYREYLVYLQGCNEQFIEAPGIRGMVMLVFTLPGFDRV
                     FKVIKDKFAPQKEMSAAHVRACYQLVKEHDRVGRMADTQEFENFVLEKRHISPALMAL
                     LLQEAAEKITDLGEQIVIRHLYIERRMVPLNIWLEQVEGQQLRDAIEEYGNAIRQLAA
                     ANIFPGDMLFKNFGVTRHGRVVFYDYDEICYMTEVNFRDIPPPRYPEDELASEPWYSV
                     SPGDVFPEEFRHWLCADPRIGPLFEEMHADLFRADYWRALQNRIREGHVEDVYAYRRK
                     QRFSVRFV"
     gene            complement(20883..22187)
                     /gene="aceA"
                     /locus_tag="ACHKBL_002079"
     CDS             complement(20883..22187)
                     /gene="aceA"
                     /locus_tag="ACHKBL_002079"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004451 - isocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HFO5818956.1"
                     /translation="MKTRTQQIEELQKEWTQPRWEGITRPYSAEDVVKLRGSVNPECT
                     LAQLGAAKMWRLLHGESKKGYINSLGALTGGQALQQAKAGIEAVYLSGWQVAADANLA
                     ASMYPDQSLYPANSVPAVVERINNTFRRADQIQWSAGIEPGDPRYVDYFLPIVADAEA
                     GFGGVLNAFELMKAMIEAGAAAVHFEDQLASVKKCGHMGGKVLVPTQEAIQKLVAARL
                     AADVTGVPTLLVARTDADAADLITSDCDPYDSEFITGERTSEGFFRTHAGIEQAISRG
                     LAYAPYADLVWCETSTPDLELARRFAQAIHAKYPGKLLAYNCSPSFNWQKNLDDKTIA
                     SFQQQLSDMGYKFQFITLAGIHSMWFNMFDLANAYAQGEGMKHYVEKVQQPEFAAAKD
                     GYTFVSHQQEVGTGYFDKVTTIIQGGTSSVTALTGSTEESQF"
     gene            complement(22217..23818)
                     /gene="aceB"
                     /locus_tag="ACHKBL_002080"
     CDS             complement(22217..23818)
                     /gene="aceB"
                     /locus_tag="ACHKBL_002080"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006097 - glyoxylate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HFO5818957.1"
                     /translation="MTEQATTTDELAFTRPYGEQEKHILTAEAVEFLTELVTHFTPQR
                     NKLLAARIQQQQDIDNGTLPDFISETASIRDADWKIRGIPADLEDRRVEITGPVERKM
                     VINALNANVKVFMADFEDSLAPDWNKVIDGQINLRDAVNGTISYTNEAGKIYQLKPNP
                     AVLICRVRGLHLPEKHVTWRGEAIPGSLFDFALYFFHNYQALLAKGSGPYFYLPKTQS
                     WQEAAWWSEVFSYAEDRFNLPRGTIKATLLIETLPAVFQMDEILHALRDHIVGLNCGR
                     WDYIFSYIKTLKNYPDRVLPDRQAVTMDKPFLNAYSRLLIKTCHKRGAFAMGGMAAFI
                     PSKDEERNNQVLNKVKADKSLEANNGHDGTWIAHPGLADTAMAVFNDILGSRKNQLEV
                     MREQDAPITADQLLAPCDGERTEEGMRANIRVAVQYIEAWISGNGCVPIYGLMEDAAT
                     AEISRTSIWQWIHHQKTLSNGKPVTKALFRQMLGEEMKVIASELGEERFSQGRFDDAA
                     RLMEQITTSDELIDFLTLPGYRLLA"
BASE COUNT         5590 a         6313 c         6255 g         5740 t
ORIGIN      
        1 ttccgttaaa tcacagacaa ggggatttat ctgataaaaa atgccgacat aacgtcggca
       61 ttgtttttaa ggctatttaa tcaatcgtac tatgtattaa ttgccgaata cgatgtacat
      121 atcggcatcg acctgtaggc ctgataagac gcgaccgcgt cgcatcaggc atcggttgcc
      181 ggatgcggcg tgaacgcctt atccggccta catatcgaca atgattaacc gcgccacgct
      241 ttatagcggt taatcagacc attggtcgag ctatcgtggc tgctgatttc tttatcatct
      301 ttcagctctg gcagaatacg gttcgccagc tgtttaccca gctccacgcc ccactggtcg
      361 aaggtgaaga tgttcagaat cacgccctga gtaaagattt tgtgctcata cagcgcaatc
      421 aatgcaccca ggctgaacgg agtgatttca cgcagcagga tggagttggt cgggcggtta
      481 ccttcgaaca ctttgaacgg caccacgtag tcaagcgttg ccggatcttt accctgatca
      541 cgatattcct gctcaaccac ttcgcgggat ttaccaaacg ccagcgcttc ggtctgagca
      601 aagaagttag acagcagttt ctggtgatga tcagagagcg ggttatgggt gatagccgga
      661 gcgatgaaat cgcacggtac cattttggtt ccctggtgga tcagctgata gaacgcgtgc
      721 tgaccgttcg tgccaggttc accccagata atcgggccag tctggtaatc cacaacctta
      781 ccgttacggt caacatactt accgttggat tccatattgc cctgctggaa gtacgccgcg
      841 aaacggtgca tatactggtc atacggcaga atcgcttcag tttccgcgcc aaagaaattg
      901 ttgtaccaga tgccaatcag cgccagcaag acaggcaggt ttttctcggc aggcgtggtg
      961 gagaaatgct tgtccatcgc gtgtgcgcca gaaagcagtt caacaaaatt atcaaagccg
     1021 atggagagaa caatcgacag gccaattgct gaccacaaag agtaacggcc gccaacccag
     1081 tcccagaact cgaacatgtt ggcagtatca ataccaaact cgccaacggc tttggcattg
     1141 gtggaaagcg ccgcaaagtg tttcgcaacg tgtttttcat caccagccgc tttcaggaac
     1201 cagtcacgcg cgctgtgggc gttggtcatg gtttcctgag tggtgaaggt tttagatgct
     1261 accaggaaca gcgtggtttc cgggtttact tttttcagca cttccgcgat gtgagtccca
     1321 tcgacgttag aaacaaagtg catgttcagg tggtttttgt atggacgcag tgcttcagtc
     1381 accatgtacg ggccgaggtc agaaccgccg atcccgatgt tcactacgtc agtgattgct
     1441 ttgccggtat agcctttcca ctcaccggaa ataatcgctt ccgagaaggt tttcatcttc
     1501 tccagcaccg cgttgacttc tggcattacg tctttgccat caaccagaat cggggtattg
     1561 ctacggttac gcagcgctac gtgcagcacg gcgcggtttt cagtgcggtt gatcttctcg
     1621 ccagagaaca tcgacttaat cgcgcccgcc agatcgcact ctttcgccag atcttgtaat
     1681 ttcgccagcg tctcttcagt gatgcggttt ttggagtaat ccaccagcat ctgatcgtcg
     1741 aatgttgcgg agaacttaga aaaacgatcg ccgtctttag caaaaagatc ggcgatcgta
     1801 acgtctttca tttcatcgaa gtgtttctgt agtgcctgcc aggcagcggt ctgcgttgga
     1861 ttgatgtttt tcattagcaa tactcttctg attttgagaa ttgtgacttt ggaagattgt
     1921 agcgccagtc acagaaaaat gtgatggttt tagtgccgtt agcgtaatgt tgagtgtaaa
     1981 cccttagcgc agcgaagcat ttattagctg aactactgac cgccaggagt ggatgaaaaa
     2041 tccgcatgac cccatcgttg acaacggccc cgctcaccct ttatttataa atgtactacc
     2101 tgcgctagcg caggccagaa gaggcgcgtt gcccaagtaa cggtgttgga ggagccagtc
     2161 ctgtgataac acctgagggg gtgcatcgcc gaggtgattg aacggctggc cacgttcatc
     2221 atcggctaca ggggctgaat cccctgggtt gtcaccagaa gcgttcgcag tcgggcgttt
     2281 cgcaagtggt ggagcacttc tgggtgaaaa tagtagcgaa gtatcgctct gcgcccaccc
     2341 gtcttccgct cttcccttgt gccaaggctg aaaatggatc ccctgacacg aggtagttat
     2401 gtctgaaatt gttgtctcca aatttggcgg taccagcgta gctgattttg acgccatgaa
     2461 ccgcagcgct gatattgtgc tttctgatgc caacgtgcgt ttagttgtcc tctcggcttc
     2521 tgctggtatc actaatctgc tggtcgcttt agctgaagga ctggaacctg gcgagcgatt
     2581 cgaaaaactc gacgctattc gcaacatcca gtttgccatt ctggaacgtc tgcgttaccc
     2641 gaacgttatc cgtgaagaga ttgaacgtct gctggagaac attactgttc tggcagaagc
     2701 ggcggcgctg gcaacgtctc cggcgctgac agatgaactg gtcagccacg gcgagctgat
     2761 gtcgaccctg ctgtttgtcg agatcctgcg cgaacgcgat gttcaggcac agtggtttga
     2821 tgtacgtaaa gtgatgcgta ccaacgaccg atttggtcgt gcagagccag atgtagccgc
     2881 gctggcggaa ctggccgcgc tgcagctgct cccacgcctc aatgaaggct tagtgatcac
     2941 ccagggattt atcggtagcg aaaataaagg tcgtacaacg acgcttggcc gtggaggcag
     3001 cgattatacg gcagccttgc tggcggaggc tttacacgca tctcgtgttg atatctggac
     3061 cgacgtcccg ggcatctaca ccaccgatcc acgcgtggtt tccgcagcaa aacgcattga
     3121 tgaaatcgcg tttgccgaag cggcagagat ggcaactttt ggtgcaaaag tactgcatcc
     3181 ggcaacgttg ctacccgcag tacgcagcga tatcccagtc tttgtcggct ccagcaaaga
     3241 cccacgcgca ggtggtacgc tggtgtgcaa taaaactgaa aatccgccgc tgttccgcgc
     3301 gctggcgctt cgtcgcaatc agactctgct cactttgcac agcctgaata tgctgcattc
     3361 tcgcggtttc ctcgcggaag ttttcggcat cctcgcgcgg cataatattt cggtagactt
     3421 aatcaccacg tcagaagtga gtgtggcatt aacccttgat accaccggtt caacctccac
     3481 tggcgatacg ttgctgacgc aatccctgct gatggagctt tccgcactgt gccgggtgga
     3541 agtggaagag ggtctggcac tggtcgcgtt gattggcaat gacctgtcaa aagcctgcgg
     3601 cgttggcaaa gaggtattcg gcgtactgga accgttcaac attcgcatga tttgttacgg
     3661 cgcatccagc cataacctgt gcttcctggt gcccggcgaa gatgccgagc aggtagtgca
     3721 gaagctgcat tttaatttgt ttgagtaaac acagtatggc ccggaagaca ttatttcggg
     3781 ccgtattgtt tttcttgtca ttatgcccat aaataaacga gcctgtactc tgttaaccag
     3841 cgtctttatc ggagaataat tgcctttaat tttttatctc tttctctaac taattagaga
     3901 aagagataaa tagttaagag aaggcaaaat gaatattatc agttctgctc gcaaacgaat
     3961 tcccgtacac tgaactctct ggctcactga gggcaccatc atggcactcc cccgtattac
     4021 ccaaaaagag atgactgaac gcgagcagcg tgaacttaaa acactgctgg atcgggcgcg
     4081 gattgcgcat ggtcgcgtac tgaccaactc cgaaaccaac agcattaaga aagagtacat
     4141 cgataaatta atggtcgagc gtgaggctga agcgaaaaaa gcccgccaat tgaagaaaaa
     4201 gcaggcttat aaaccggatc ctgaggcgtc atttagctgg tcagcaaata cgtcgacgcg
     4261 cggcaggcgc tgattaacgc cccttctttt tgcgccccgg cgaggtaaaa cgcttaccgt
     4321 tggacgccgg gcgaccgcct ttttccgccg ttttttccat cttaacgact ggacgtttga
     4381 tgcctaccgt tttcggtttg gccttcgctt taggttttgc ctcggaagag gaattttcga
     4441 tgagcttaaa gaggtcgatt aactcatcgt cggttaaatc gcgccactcc cccagcggaa
     4501 tgccgcttaa actgacgttc ataatgcgcg tacgttccag ctttttcact tcgtaaccaa
     4561 agtgctcgca catgcgacgg atctgacggt tcagcccctg caccagggta atgcggaaga
     4621 caaatggcgc ttcttttttg actttgcact ttttagtcac tgtcccgagg attggcaccc
     4681 ccgcccccat accacgaata aattcatcgg taatcggttt atcgaccgtc accagatact
     4741 ctttctcatg atcgttaccc gcacggagga tcttattcac cagatcgccg tgattggtga
     4801 ggaaaatcag cccctgggag tctttatcca ggcggccaat cgggaatacg cgtttgctgt
     4861 ggttaacgaa atcgacaatg ttatcgcgct cgccatcttc ggtggtgctg acaataccga
     4921 cgggcttgtt cagggcgata agtaccaaat cttcggcttc ccgaggttca atcaactgac
     4981 catttacttt cacaacgtcg ccgggtttca cctgatcgcc aatggtggct cgcttgccat
     5041 taaggaacac attgccttgc tcgatatagc gatccgcttc gcggcgtgag caaattccgc
     5101 tttcgctgat gtatttattt aaacggactg atgagtcggg cagcataaat tctcctgtaa
     5161 aacgcggaat ataccgtacc tcttagcggg gaaaaacagg gttatgcggc aacgtcgaaa
     5221 ttaaatgtga tctgccacgc cttttatcac aaaagtgtct ccggaagtac gatgcaaatt
     5281 tgcctggcaa aataaggata atcaacatat tacgaatcgc tgcacacctg tgctgatgac
     5341 tactacgcag agaaacaaat gtgcaagaga aagcgctatt tctccccatg caaagagtga
     5401 aaaatcgctt tgaaaggccc tcatgatggg gttatatcag atcaagcggt gagaaagcgt
     5461 atttgcacaa atgtgcagaa ccaggaggcg gcaatggaaa acagtgacga tatccgtttg
     5521 attgtgaaga ttgcccaact ctattacgaa caggatatga cgcaggcgca aatcgcgcgc
     5581 gaactgggta tttaccgcac caccatcagc cgcttgctta aacgaggccg cgatcaggga
     5641 attgtcacca tcgccatcaa ctatgactac aacgaaaatc tctggctgga gcagcaactg
     5701 aagcaaaagt ttggcctgaa agacgttgtg gtggtgtcag gaaatgatga ggatgaagag
     5761 actcaactgg cgatgatggg gttacacggc gcgcaactgc tggatcgctt gctggaacct
     5821 ggcgatattg tcggtttttc ctggggccgc gcggtgaacg cactggttga aaacttgccg
     5881 caggcggggc aatcgcggca gttaatctgc gtaccaatta ttggcggacc gtccggtaaa
     5941 ctcgaaagcc gctatcatgt aaacacatta acctacagcg cggcagcgaa gttgaaaggg
     6001 gaatcgcatc tcgcggattt tccggctctg ctggataacc cattaattcg taatgggatc
     6061 atgcagtctc agcactttaa aaccatctct gcctactggg ataatctgga tgtcgccctg
     6121 gtgggaattg gctcaccggc cattcgcgac ggcgctaact ggcatgcgtt ttatggtggt
     6181 gaagagagtg acgacctgaa tgcccgccag gttgctggcg atatttgctc gcgctttttt
     6241 gatattcacg gcgcaatggt tgaaacaaat atgagcgaaa aaacactctc tatcgaaatg
     6301 aataaattaa agcaggcgcg gtattccatt ggcattgcca tgagtgaaga aaaatacagc
     6361 ggaattattg gtgcactgcg tggaaaatat attaattgtc tggtaacgaa tagcagcaca
     6421 gctgaactat tactgaaata aaaatcttcc cgatgtttac cgtaataaaa attacggcgg
     6481 gtctctatta tctaaaatca ggagtctgtt atgcaaacgt ggttaaattt gcaggataaa
     6541 atcattattg tcaccggtgg cgcatccggc attggtctgg cgattgtgga tgaattatta
     6601 gcacaaggcg cgaatgtaca gatggtcgat attcacggtg gcgatggaca atatgaaggt
     6661 cataaaggct atcagttttg gccgaccgat atttccagcg ccaaagaggt aaatcatacg
     6721 gtagcggaaa ttatccagcg ttttggtcgc atcgacggtc tggtcaataa cgccggggtc
     6781 aatttcccgc gtctgctggt cgatgagaaa gcgcctgccg ggcagtatga actcaacgaa
     6841 gctgcattcg aaaaaatggt caatatcaac cagaaaggcg tttttctgat gtcgcaggcg
     6901 gtggcgcgac agatggtcaa acaacatgat ggcgtgattg tgaatgtttc ctcagaaagt
     6961 gggctggaag gctcagaagg ccaaagctgt tacgccgcta ccaaagccgc gcttaatagc
     7021 ttcacgcgat cctggtcgaa agaattgggt aaacacggta tccgtgtagt cggtatcgcg
     7081 ccggggattc tggaaaaaac caggctacgc acgccggaat atgaagaggc gctggcgtgg
     7141 acgcgcaata tcaccgtcga gcagctgcgt gaaggctata ccaaaaacgc cattcctatt
     7201 gggcgcgccg gaagattagc agaagtcact gattttgttt gttatctgct gtctcaacgc
     7261 gccagctata tcaccggagt aaccactaac attgcgggcg gcaaaacgcg cggctaagga
     7321 ggctttatgg tcaacgccat tttttgtgcc cacggcaaat tagcttgcgc catgctggaa
     7381 tcggttcaga tggtttacgg caacgccaat gttgaagccg ttgaatttgt gcccggtgaa
     7441 aacgccggag acatcgtagc aaagctggaa aagttagtaa gcattcacaa tcaagatgag
     7501 tggctgattg ccgtggattt acagtgcggc agcccgtgga atgcggcagc gatgcttgcg
     7561 atgcgcaatc ctcgtctgcg ggtaatcagc ggcctttctc tgccgctggc gctcgaactg
     7621 gttgataacc agcacagcat gaatgttgat gaactgtgtg aacacctgac gcaaatcgca
     7681 aagcaaacct gcgttgtctg gaaacaactg gcaacggcag aggaggattt ctgatgaaca
     7741 ttacgctcgc ccgcattgat gaccgtctga tccacggcca ggtcactacc gtctggtcga
     7801 aagtggctaa tgcccagcac attattatct gcaatgacga agtttataac gatgaagtgc
     7861 gccggacatt attgcgtcag gctgcgccac cgggaatgaa ggttaacgtg gtcaatattg
     7921 aaaaagccgt cgcggtttat cacaacccgc aatatcagga cgaaaccgtt ttttatcttt
     7981 tcactcgccc acaggatgcc ttagcgatgg ttcgccaggg ggtaaaaata gacaccctga
     8041 atattggcgg catggcctgg cggcccggta aaaaacagtt aaccaaagcg gtttcattag
     8101 atgatgatga tattaacgca tttcatgagt taaataatct tggcgtcata ctggatttac
     8161 gcgtcgtagc gtcagatccc tcaatcaata ttatcgacaa aataaacgaa cagttaatcg
     8221 caaattaaat aatagcgcct gtgtacatat gctttaacag gcagggatga cgacacttaa
     8281 aaggtgacaa atcatggaaa taagtaccct acaaatcatt gctatatttc ttttttcctg
     8341 tattgccgga atgggcagcg tgctggatga atttcagact catcgtccgt taattgcctg
     8401 tacggtgatt ggtttaattc tcggtgattt aaaaaccgga attatgctcg gtggtacgct
     8461 ggaattgata gctctcggct ggatgaacgt cggcgcggcg caatctccgg attctgcact
     8521 cgccagcata atctccgcca ttctggttat cgttggtcag caaagcatcg ccaccggaat
     8581 cgccatcgcg ttgcctgtgg ctgcggcagg ccaggtgctg acggtgtttg cccgtaccat
     8641 caccgtagtg ttccagcacg cggcggataa agcagcggaa gaagcacggt ttcgcactct
     8701 cgatattctg catgtctccg cgcttggcgt gcaggcactg cgcgttgcta ttccggcact
     8761 gattgtctcg ctgttcgtca gcgctgatat ggtgagcaat atgctgagcg ccattcccga
     8821 atttgtgacc cgtggactgc agattgctgg cggttttatc gtggtggtcg gttacgccat
     8881 ggtgcttcgc atgatgggcg tgaaatattt gatgcctttc tttttcctcg gtttcctcgc
     8941 aggtggctac ctcgatctca gtctgctggc gttcggtggc gtcggcgtga tcatggccct
     9001 gctctacatc cagctaaatc cacagtggcg taaagctgaa cctcaactcc aggccaccgc
     9061 ctccaccgcc cttgaccaac ttgacgatta acggagccat catggaacag agaaaaatta
     9121 cacgcagcga tctggtgagc atgtttctgc gctccaacct gcaacaggcg tcctttaact
     9181 ttgaacgtat tcacgggctg ggtttttgct acgacatgat ccccgccatc aagcgacttt
     9241 acccattaaa agaggatcag gttgcggcgc tcaggcgaca cctggtgttc ttcaatacca
     9301 cgccagccgt atgtggcccg gtcatcggcg tcactgccgc catggaagag gcgcgggcca
     9361 acggcgcgga aattgatgac ggtaccatta acggcatcaa agtcggtctg atgggaccat
     9421 tggcaggagt tggcgatcca ctggtctggg gaacgctgcg cccgattacc gccgcgctcg
     9481 gcgcatctct ggcactttcg ggcaacattc tcggcccgct gctgtttttc tttattttca
     9541 atgcggtgcg tctggcgatg aagtggtatg gcctacagct cggctttcgc aaaggggtga
     9601 atatcgtcag cgatatgggc gggaatgtgc tgcaaaaact caccgaaggc gcgtcgattc
     9661 tcggactgtt tgtgatgggc gtgctggtga ccaaatggac gtcaatcaac gtaccgttgc
     9721 tggtttcaca aacgcatgcc gccgatggct ccaccgtcac catgaccgtg cagaacattc
     9781 tcgaccaact ttgccctggt ttgctggcgc tcggtctgac gctgctgatg gttcgtctgc
     9841 tcaacaaaaa aattaacccg gtatggctga ttttcgccct gtttggttta gggattatcg
     9901 gtaatgcgct gggcttcctg tcctgattct tcgccccggc acgactgccg gggtcatcgc
     9961 tcaacatgag gtggtttatg aaaacaacag ctctgcgtct ttatggcaaa cgtgacctgc
    10021 gtctggaaac ctttgacctt cctgaaatgc aggaggatga aatcctcgca acggtggtca
    10081 ctgacagcct gtgcctctct tcctggaaag aggccaatct gggtgaaaac cataaaaaag
    10141 tacccgacga tgtggcgacc aaccccatca tcatcggcca cgagttttgc ggcgatattc
    10201 tggccgtggg taaaaagtgg cagcacaaat tccagccggg tcagcgttat gtgattcagg
    10261 ccaacctgca actccccgac cgcccggatt gccccggcta ctccttcccg tgggtaggcg
    10321 gcgaagcgac gcatgtggtt attcccaacg aggtcatgga acaagattgc ctgctggcat
    10381 acgacggcga aacctatttt gaaggctcgc tggttgaacc gctttcctgc gtgattggcg
    10441 cgttcaacgc caactatcat cttcaggaag gtagttataa ccacacgatg gggattcgcc
    10501 cgcaagggcg cacgctgatc ctcggcggca ccggcccgat ggggctgctg gcgattgatt
    10561 atgcgttgca tggaccggtt aacccgtcgc tgctcgtcat taccgatacc gacaacgaca
    10621 aattgagtta tgcacgcaag cactatccat cagaaccgca aacactgatt cattatctca
    10681 atgccgccga tgcagcattt gatacgttaa tggcactgag tggcggtcac ggcttcgatg
    10741 atattttcgt ctttgtgcct aatgaaggac tggtgactct cgcctcttcc ttgctggcga
    10801 cagatggttg tctgaatttc ttcgccggac cgcaggataa gcacttcagc gcgccaatta
    10861 atttctacga tgtgcattat gcatttaccc actacgtggg cacgtcaggc ggcaataccg
    10921 acgacatgcg cgcagcggtc aaattgattg aagagaaaaa agtgcaggcc gcaaaagtgg
    10981 taacacatat tcttgggctg aatgccgcgg gcgaaaccac gcttgaattg cctgccatcg
    11041 gcggcggcaa aaagctggtg tataccggga aatacctgcc gcttatgtca ctcacgcaga
    11101 ttcaggatca agcactggcg gcaattctgg cgcgtcatca ggggatctgg tccggtgagg
    11161 cggagcaata cctgctcgcc catgcagagg caatttccca tgattaatcg cgctacttaa
    11221 ctctgcatgt cgctggctgg tcgccgcggc aacttcgcga cctgatgtgc ggctgattgc
    11281 tgaagcggca caatgtgcac gagaaggata aaaaaacatc agcgccttaa ggggcgctca
    11341 aattggtggc aaagagcaca ttgtttatgc cggatgcgga atgaagcctt attcggccta
    11401 cataatcttg aaaattcaat atattgcagt tatctggtag gccaggtaag cgtagcgcat
    11461 caggcaatcc tgtgtttgtc atcagtctca gcgcctgtgc aatatgtgac atcgtgagcc
    11521 atgtcagtaa gtggttaaat tcgcagtggt agcattcctt ccgtgttttc ttactaaccg
    11581 gagaagtaat ggaactgctt ttattgagta actcgacgct gccgggtaaa gcctggctgg
    11641 aacacgcgct gccgctgatt gctgaacagt tgcagggtcg ccgttctgcg gtatttattc
    11701 ctttcgcggg cgtaacgcag acctgggatg attacacagc gaaaacggct gcggttctcg
    11761 ctccgctggg tgtttctgtc accggtattc atagcgttgt cgatcccgtt gccgcgattg
    11821 aaaatgcgga aattgtgatt gtcggcggcg gtaatacttt ccagttgctg aaacagtgcc
    11881 gcgagcgcgg actgctggca ccaattactg acgtggttaa acgtggcgcg ctgtatattg
    11941 gctggagcgc tggcgctaac cttgcttgcc caaccattcg taccaccaac gatatgccga
    12001 ttgtcgatcc acaaggtttc gatgcgctga atctgttccc gttgcaaatc aacccgcact
    12061 tcaccaacgc gctgccggaa ggccataaag gtgaaacccg tgagcagcgt attcgcgaac
    12121 tgctggtcgt cgcgccagaa ctcacaatta ttggtctacc ggaaggtaac tggatcacgg
    12181 tgagtaaagg tcacgctacg ctgggtggcc cgaacaccac ttatgtgttt aaggctggtg
    12241 aagaagcggt tccgctggaa gcgggtcacc gtttttaagt gaattgccgg atgcatgatc
    12301 cggcatcttg tacatagtgc ctgatgcgac gctttacacg tcttatcagg ccttcagggt
    12361 cacggtatta atactcgtcc cgtccttcgt cttcatccgg ctgttccagc acactgtaag
    12421 ccaccgaaca aaacagcgag ttcagacgct gcatatctcc cagtaagcct aaatgtagcg
    12481 aactggtttc aatactctgc acgttttgct gatgcaggcg atcgacgtga gcatgggaat
    12541 aacggcgatt aagaatgcga aagcgatgct tgctgcggcg caaacgacgg gcgctggtaa
    12601 catcgccaga gaagaaaacc gacattgcca gttttaaatt actgagcaat tgctcataga
    12661 gcgcatccag ttccttcaag ccatcaagcg aaaatgcccg ccgtgctgcc agcgatttat
    12721 cggcaatttc actccccatg cgctcgacga tatcggaggc ctgttcaagg ttgagcgaca
    12781 tttcgatgat ctccgcccag cggcgcgact cttcttctgc cagttcctct tttggcatcc
    12841 gcgccagata cagcttaatg gcggtataga gaacgttgat gtcatcagcc agcttacgca
    12901 gctctttctc ctgccgtggc tcgccgtgca tcactttatt caacccttcc atcatctgtt
    12961 ccatggcgtc gccaatgcgc agggtttcgc gggcagcgtt cgccagagca agcgtgggcg
    13021 tatccagcgc gctgacatcc agatgtttag gccgtagctg ggtatccagt tccggttcat
    13081 cgcgaataat cgttttgcaa aaccgtgcca tcgggtcaac aaatggcagc atgaccaggc
    13141 agcgtacaag gttgtagaag acgtggaaat agatcaccaa ttccgctttt ggcaatggca
    13201 acttccccat tgtctctgcc agcaaatgaa caaacggcag gataatcagg ctacccacca
    13261 gcttaaacag caggctaccc agcgcgacac ggcgggctgc ggcattggcg gcactgttgt
    13321 tgagcatcgc cagcaggccg gaaccgaggt ttgcaccaat caccagacaa agcgccactg
    13381 ggaaagagat aatgcctgcg gcggtcagag tcgcggtcag cagtaccgcg gcaaggctgg
    13441 agtagctgat aatggcgaac atcgcaccaa tcagcgcatc cagcagaata tcgccggtca
    13501 gcgaggcaaa gatcacctga acgccgtttg cctgggtgat cggcgttacg gcctgcacaa
    13561 tcaactccag cgccagcaaa atcaacccaa gaccaatacc gacgcggccc agttgcccgg
    13621 cgcgtgactg tttgcgtccg aggaaaaaaa tcacgccgat aaaaataagt aatggtgaca
    13681 gccaggataa gtcgaaggtg agaatacgcg ccattagcgc cgtcccgaca tctgcaccca
    13741 gcacaatcac cagagccggt gcgagggcta ccagatcctg agcgacgaac gaggtcacca
    13801 gcatagtggt ggcattactg ctctgcacca gtgcggtaac gcctatcccc gcgcaaaagg
    13861 cgagcggctt tttttcgacg ctccggctaa ggacagtacg caaacgcgcg ccgaagacgc
    13921 gcattacgcc ggttcgaaca atatgagtcc cccagaccag cagggcgacg gcagaaagca
    13981 ggtgaagcag cgttaacacg agttcaggtt ctccttatcg ttatatgttc ctgaagcaat
    14041 tgaatgacca aaggaccgga aacacgctga ctggcatctt tactgtgcct tttgaccttc
    14101 gtttttccgg ctaagattat tccagcctgt ttccagtata agtgtttcat attaataaag
    14161 agacagggca ctcattgagc gccctgtttg ttgtaaggaa aagtgacaga tttgtgaatc
    14221 agtccgcgtc ataccccaga ttcggtgcca gccagcgctc aacatctgag acgctcatac
    14281 ctttacggcg ggcatagtct tcaacctgat cgcgctggat ttgcgccacc gcgtaatact
    14341 tgctgtccgg gtggctgaag taccaacccg aaaccgatgc accaggccac atggcgaaag
    14401 attctgtgag tttcatgcca gtgtgttttt ccacttccag cagctcccag atggtggctt
    14461 tttccgtatg ttccgggcag gccggataac ccggtgccgg acggatgccc tggtagtttt
    14521 cgcggatcag ctcttcgttg ctgaggttct cgttcggcgc atagccccag tagactttac
    14581 gcacacgctc atggagatac tccgcaaagg cttcggctaa acggtcggca agcgctttca
    14641 ccatgatttt gttgtaatca tcgtgctgcg cttcaaaggc atcagccagt gcgtcctctt
    14701 ccagcccgcc agtcacggca aatgcgccga tgtaatctgc tttaccagaa agcttcggcg
    14761 caacgaagtc agcgagacag tagttagcga agccggtttt ttcggtctgt tgacgcagat
    14821 ggtggctgac gttgatcaca tgggtacgcg tttcgtcacg gtagatttca atgtcatcgc
    14881 ccacacggtt tgccgggaac aggcccacca cgccacgcgg attcagcgtt ttttcggcgc
    14941 ttaatttatc cagcatgtcg ttggcgtctt taaacagccg ctgcgcctca acgcccacca
    15001 cttcatcttc cagaatgcgc ggatacttcc cggacagcga ccaggtcata aagaacggtg
    15061 tccagtcgat gtaattacgc agcgtttcga tgctggcttc gacttcctgc acaccgagac
    15121 ggtgcgccac cggcggcgtg taagcctgcc agtcaaaagc gaagtcgtta tcacgcgccg
    15181 cttccagcgt gaccggtggt gtgcgtggtt tctttcgtcc gtgctgaata cgcacggttt
    15241 cgtactcctt gcgggtacga gcaacgaaat catcgcgctg agtgtcggaa agcaacgccg
    15301 ccaccacacc aacggtgcgt gaagcgttct gcacatacac cgtcgggccg ctgtagttct
    15361 gctcaatttt caccgccgtg tgcgcttttg aggtggtcgc gccgccaatc aatagcggaa
    15421 tggtgaagcc ctgacgctcc atctctttcg ccacgttaac catctcgtcc agcgacggcg
    15481 tgatcagccc cgaaaggcca atcaaatcag cattcacttc tttagcggta cggagaattt
    15541 tttccgcagg caccatcacg ccgagatcga caatttcgta gttgttacat tgcagcacca
    15601 cgccaacgat atttttaccg atgtcgtgga catcgccctt cactgtggcg atcaccatct
    15661 tgccgttggt tttaccctgc tctttgctgg cttcaatgaa cggctcgaga tacgccaccg
    15721 cctgtttcat gacgcgcgcc gatttcacca cctgcggcag gaacattttg ccctcgccaa
    15781 acaggtcgcc gaccacgttc atgccgtcca tcaacgggcc ttcaatcact tcaatcggac
    15841 gcgtagcctg ctggcgggct tcttcggtat cctgctcgat aaactcggta atgcctttga
    15901 ccagcgagta ttccagacgt ttattcactt cccacgagcg ccactctgcc tgctgggtgt
    15961 tagcggtgtc gtcggttttg ctgccgcgat atttctcggc aagctcgagt aaacgctcgg
    16021 tgccatcgtc gcgacggttg aggatcacat cctccaccgc gtcgcgtagc tcggtaggca
    16081 ggtcgtcgta aatcgccagt tgcccggcgt tgacgatccc catatccatg ccattgcgaa
    16141 tagcgtagta gaggaacacc gcgtgaatgg cttcgcgcac cggatcgttg ccacggaatg
    16201 agaaagaaac gttagaaacg ccgccggaaa tcagtgcgtg cggcagttca cgtttgatgt
    16261 cttcacacgc gccgataaag tcctgcgcgt aattgttgtg ctcttcaatg ccagttgcga
    16321 ccgcgaagat gttcgggtcg aagatgatat cttcagccgg gaagccaacc tcttcggtga
    16381 ggattttgta cgcccgacgg caaatctcga ttttccgtgc gcgggtatcg gcctggccct
    16441 gttcgtcaaa agccattacc acgactgccg caccgtagcg acgcaacagt ttcgcgtgat
    16501 ggataaaggc gtcgacgccc tctttcatcg agatagagtt aacaatgcct ttgccctgga
    16561 tacacttcag acctttttca ataacgtccc attttgagga gtcgatcatt atcggcacgc
    16621 gagcgatatc cggttcaccg gcaatcagat tgagaaaacg caccatcgcc gcttcggcgt
    16681 cgagcatccc ttcatccatg ttgatatcga taatctgcgc accgttttcc acctgctgac
    16741 gcgccacatc cagcgcctcg ctgtatttct cttctttgat caggcgcttg aacttagcgg
    16801 aaccggtgac gttggtgcgt tcacccacgt tcacaaacag gctatcttcg ccaatgttca
    16861 gcggctccag gccggacaaa cggcaggcta cggggatttc cggcagtttg cgcggcgcta
    16921 atccttctac tgcacgactc atcgcggcaa tatgttgtgg cgtggtgcca cagcagccgc
    16981 cgacgatatt gagaaagccc gcttgcgccc attcacgtat ctgttttgcc atcgtgtcgg
    17041 cgtcgagatc gtactcacca aaggcgttgg gtagcccggc gttcgggtgc gctgtgacgt
    17101 agcattccgc aatccgtgat aattcctgca cgtactggcg cagttcgtct ggccccagcg
    17161 cacagttaag gccgaaagtc agcgcttcgg cgtggcgcag agagttataa aacgcttcgg
    17221 tggtctgccc ggagagcgtg cgcccggagg cgtcggtgat ggtgccggag atcataatcg
    17281 gcagctcaac gcccagcgct tcaaactccg ttttcaccgc aaagaccgcc gctttggcgt
    17341 taagggtgtc gaaaacggtt tcaatcagaa tcaggtcggc accaccttcc accagcgctt
    17401 tggtggactc tcgataagcc gccaccagcc cgtcaaaagt gatattacga aatgccggat
    17461 cgttgacatc cggagaaata gacgccgtgc ggttggtcgg gccgagaaca ccggcaacgt
    17521 agcgcggttt ctccggcgtg cgcgcggtcc actcatcagc acaggcgcgc gccagtttcg
    17581 ccgccgcaaa gttgatttcc gccgacagga attccatctg gtaatccgcc atcgcaatgg
    17641 ttgtggagtt gaaggtgttg gtttcgatga tatccgcgcc cgcttcaaag taggcgttgt
    17701 ggatagcggc gatcacttcc ggtttgctga gcaccagcag gtcgttgttg cctttgagat
    17761 cgcatggcca gtcggcaaag cgttcaccac gaaaatcggc ttcgttcagt cgatagctct
    17821 ggatcatggt gcccataccg ccgtccagca ccagaatacg ttcatttaac tgcgcacgca
    17881 gttgttccac tttgctgctc acacttgctc ccgacacgct caacccagat ttaataaaaa
    17941 ttcaacaaac catactggca taaacgcatc tgtggtaaaa gcgaccacca cgcaacatga
    18001 gatttgttca acattaactc atcggatcag ttcagcaact attgcattag ctaacaataa
    18061 aaatgaaaat gatttccacg atacagaaaa aggagactgt catggtcgca cccattcccg
    18121 caaaacgcgg cagaaaaccc gccgttgcca ccgcaccagc gactggacag gttcagtctt
    18181 taacgcgtgg cctgaaatta ctggagtgga ttgccgaatc caatggcagt gtggcactca
    18241 cggaactggc acagcaagcc gggttaccca attccacgac ccaccgcctg ctaaccacga
    18301 tgcagcaaca gggctttgtc cgtcaggtcg gcgaactggg tcactgggca atcggcgcac
    18361 acgcctttat ggtcggtagc agctttctcc agagccgtaa tttgttagcg attgttcacc
    18421 ctatcctgcg caatctaatg gaagagtctg gcgaaacggt caatatggcg gtgcttgatc
    18481 aaagcaatca cgaagcgatt attatcgacc aggtacagtg tactcacctg atgcgaatgt
    18541 ccgcgcctat cggcggtaaa ttgccgatgc acgcttccgg tgcgggtaaa gcctttctgg
    18601 cccaactgag cgaagaacag gtgacgaagc tgctgcaccg caaagggtta catgcctata
    18661 cccacgcaac gctggtgtct cctgtgcatt taaaagaaga tctcgcccaa acgcgcaaac
    18721 ggggttattc atttgacgat gaggaacatg cgctggggct gcgttgcctt gcagcgtgta
    18781 ttttcgatga gcaccgcgaa ccgtttgccg caatttcaat ttccggaccg atttcacgta
    18841 ttaccgatga ccgcgtgacc gagtttggcg cgatggtgat taaagcggcg aaggaagtga
    18901 cgctggcgta cggtggaata cgctgacttt ttcgcctctg atttatacga atcgaacgct
    18961 aaaccgctgc ttgcgccgat acgcataaac atcttccaca tgcccttcac gtatgcggtt
    19021 ttgtagtgcg cgccagtaat cagcgcggaa caagtcggcg tgcatctctt caaatagcgg
    19081 accaatacgc gggtcggcac ataaccagtg acggaactct tccgggaaaa catcgcccgg
    19141 cgagacgctg taccacggtt cgctggcaag ttcgtcctcc gggtagcgcg gcggcgggat
    19201 gtcgcggaag ttcacttccg tcatgtagca aatttcgtcg taatcataaa acaccacgcg
    19261 cccgtgacgg gtgacaccga agtttttaaa cagcatgtcg ccagggaaaa tattagcggc
    19321 ggcaagctgg cgaatagcgt taccgtattc ttcaatggca tcgcgcaact gctgaccttc
    19381 cacttgttcc agccagatat tgagcggcac catccgccgc tcaatataaa gatggcgaat
    19441 cacaatttgt tcgccgagat cggtgatttt ttccgctgct tcctggagca gtaatgccat
    19501 taatgccggg gaaatatgcc gcttctccag cacaaagttt tcaaactcct gggtgtccgc
    19561 cattcggccc acgcgatcgt gctctttcac cagttgatag caggcacgaa cgtgagcggc
    19621 agacatctct ttctgcggcg cgaacttgtc tttgatgact ttgaataccc gatcaaagcc
    19681 cggcagcgta aacaccagca tcaccatgcc acgaataccc ggcgcttcaa tgaactgctc
    19741 attacagccc tgtagataaa cgagatattc gcggtagctt tcggttttgg cgtgcttctg
    19801 gcagccgata gccatataca attcagcggt ggttttacct ggcagaattt cccgtagcca
    19861 ctcgaccagc gctgcgggca gcggtgcata aaccataaaa taagaacgcg caaagccaaa
    19921 aacaatgctc gcttcggcgg tggtcgtcag gcaggtatca ataaataact cgccgtcgtc
    19981 cgtctggtgg atcggcagca aaaatggcaa tgtgccggaa ggcgtgatca gattgcctac
    20041 cagccaggcg gctttattgc ggtaaaacag ttcgttcgcc acctgtaaat gactttccgc
    20101 gaggttgtct gtccccagcg tttccgtcag atggcgaacg atgtaatgaa tgtcacggct
    20161 tttattctgc caacgcagac gcagcggcag atcgctgata acgcgcatca gcagagattc
    20221 ccagccgtga tcggggtgaa agtctttcgc cagcgggcgg ggaatggtac gaaaacggcg
    20281 ctctggctgg gagctaaaga taaaaagccg ctcgggagta agcgagcggt ggtcaaataa
    20341 cctacagtac acggagttga aaaagctctc cgcaatctcg aagcgcgggt aatccggcaa
    20401 cagccgcgtg taatgctctt tgacgcgcag taaaaatgcc gcgtccgtgc tttggccgtt
    20461 ggtaatgcag cgcagttgct ccacgaccag acccacgtga tgatcgtaaa gatgaatacg
    20521 gtttttcatc gcctgctgga cagcatgcca gtcggcttgc tcgaaacgct gctgcgcccc
    20581 ggaagtcact tcgaggaatc gaccatactg agcgtcgaaa ccttgcaaaa tggtttgtgc
    20641 aatcagtaat tccaggccac gcggcatttg ttgccctcgt cgggagcaga gaattgccgg
    20701 ataagacgcg taagcgtcac atcaggcgct gattgccgga tgcggcgtag acgccttatc
    20761 cggcctacgt tcggcaacgg ctgtaggcat gataagacgc gcaagcgtcg catcaggcgc
    20821 tgattgccgg atgcggcgtg aacgccttat ccggcctaca gtcagcaacg tttatcggtt
    20881 gcttagaact gcgattcttc ggtggagcca gtcagtgcgg taaccgacga cgtgccgccc
    20941 tgaataatgg tcgtcacttt atcgaagtaa cctgtaccca cttcctgttg gtgagatacg
    21001 aaggtatagc catctttcgc ggcggcaaat tccggctgct gcactttctc aacatagtgc
    21061 ttcataccct cgccctgggc ataggcgttt gccaggtcaa acatgttgaa ccacatgctg
    21121 tggatacctg ccagggtgat gaactggaac ttgtagccca tatccgacag ctgctgctgg
    21181 aagctggcaa tagttttgtc gtcgaggttt ttctgccagt tgaacgacgg cgagcagtta
    21241 taagccagca gtttgcccgg atatttcgcg tggatggctt gtgcaaagcg acgcgccagt
    21301 tccaggtccg gcgtggaggt ttcacaccag accaggtcag catatggcgc atacgccagg
    21361 ccacggctga tcgcttgctc aatacccgca tgagtacgga agaagccttc actggtacgc
    21421 tcgccagtaa taaattcgct gtcatatggg tcgcaatcgg aggtaatcag atccgccgca
    21481 tcggcatcgg tacgagcaac cagcagggtt ggaacgcccg tcacatcagc tgccagacgc
    21541 gccgcgacca gtttctgaat agcttcctga gttggcacca aaactttgcc gcccatgtga
    21601 ccacatttct tcactgatgc cagctgatct tcgaagtgaa ctgccgctgc accggcttca
    21661 atcatcgctt tcatcagttc aaaggcattc agtacaccgc caaaaccggc ctccgcatcg
    21721 gcgacgatcg gcaggaagta atcgacatag cgcggatcgc ccggctcaat gcccgcggac
    21781 cattggatct gatccgcacg gcggaaagta ttgttgatcc gctccaccac cgctggcacc
    21841 gagtttgccg gatagagcga ctgatccgga tacatgctgg ccgccaggtt agcgtccgcc
    21901 gctacctgcc atcccgacag atagactgct tcaatacccg ctttcgcctg ttgcagcgcc
    21961 tgaccgccag tcagtgcacc gaggctgttg atgtagcctt ttttcgactc gccgtgcagc
    22021 agacgccaca ttttcgccgc gcccagttgc gccagggtgc attccggatt gactgaaccg
    22081 cgtaatttca ccacatcttc cgcgctgtac gggcgagtaa tgccttccca acgcggttga
    22141 gtccactctt tctgtaattc ttcaatttgt tgtgtacggg ttttcatgtg cagatgctcc
    22201 atagttatgt ggtggtttac gctaacaggc ggtagcctgg cagggtcagg aaatcaatta
    22261 actcatcgga agtggtgatc tgttccatca agcgtgcggc atcgtcaaaa cgcccctggg
    22321 agaaacgttc ttcgcccagt tcgctggcaa tgactttcat ctcttcgccc agcatctggc
    22381 ggaacaaggc tttagtcacc ggtttgccat tgctcaacgt tttttgatga tggatccact
    22441 gccagattga ggtacgggaa atttcagccg tcgccgcatc ttccatcagg ccataaatcg
    22501 gcacacagcc attgccagag atccacgctt cgatgtactg cacagccacg cgaatgttgg
    22561 cgcgcatacc ttcttcggtg cgttcaccat cacaaggtgc cagcagctga tcggcagtaa
    22621 tcggcgcgtc ttgttcgcgc atcacttcaa gctgattttt acgggagccg agaatgtcgt
    22681 tgaataccgc cattgccgta tccgcaaggc ctgggtgggc aatccatgtg ccatcgtgac
    22741 cgttattagc ttccagcgat ttatccgctt ttactttgtt gagcacctgg ttattgcgct
    22801 cttcatcttt gctcggaata aatgccgcca taccgcccat cgcaaaagca ccgcgtttat
    22861 ggcaggtttt aatcaacagg cgtgagtaag cattcaggaa gggtttatcc atcgtcactg
    22921 cctgtctatc tggcaggacg cgatcgggat agtttttcaa tgttttgata taactgaaga
    22981 tgtaatccca acgaccgcag ttcagaccaa caatatggtc acgcagcgcg tgaaggattt
    23041 catccatctg gaacacggcg ggcagcgttt caatcagcaa cgtcgccttg atggtgccgc
    23101 gcggcagatt aaagcgatct tctgcatagc tgaagacttc gctccaccag gccgcttcct
    23161 gccaggactg ggttttcggc agatagaaat agggaccgct gccctttgcc aacagtgcct
    23221 gatagttgtg gaagaaatag agcgcaaaat caaacaggct accggggatt gcctcaccac
    23281 gccaggtgac atgtttttcc ggcaagtgca gaccgcgtac ccgacaaatc aaaaccgctg
    23341 gattgggctt gagctgataa attttgcctg cttcattggt atagctgatg gtgccgttaa
    23401 ccgcatcgcg caggttaatt tgcccatcga tcactttgtt ccagtctggt gccagtgaat
    23461 cttcgaaatc ggccataaag actttcacat tggcgttgag cgcgttgatc accatcttgc
    23521 gctcaaccgg gccggtaatc tctacccgac gatcttctaa gtccgcagga atcccgcgaa
    23581 ttttccagtc agcatcacga atggaagctg tttccgaaat aaaatcaggc aacgttccgt
    23641 tatcgatatc ctgctgctgc tgaatgcgcg ctgccagaag tttattgcgt tgtggcgtaa
    23701 aatgcgtcac cagctcagtc agaaattcta ccgcttcggc agtaagaata tgcttctcct
    23761 gctcgccata cggccttgtg aaagccaatt catcggttgt tgttgcctgt tcagtcatcg
    23821 tgcagctcct cgtcatggat ccgaaaactt ccccactgtg aacgaaggat cgttgtgcgc
    23881 ttttcgttca gcacaact
//
LOCUS       DAZFLD010000063        22995 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.063, whole genome shotgun sequence.
ACCESSION   DAZFLD010000063 DAZFLD010000000
VERSION     DAZFLD010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22995
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.063"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            15..944
                     /gene="aaeR"
                     /locus_tag="ACHKBL_002081"
     CDS             15..944
                     /gene="aaeR"
                     /locus_tag="ACHKBL_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AaeR"
                     /protein_id="HFO5818958.1"
                     /translation="MERLKRMSVFAKVVEFGSFTAAARQLQMSVSSISQTVSKLEDEL
                     QVKLLNRSTRSIGLTEAGRIYYQGCRRMLHEVQDVHEQLYAFNNTPIGTLRIGCSSTM
                     AQNVLAGLTAKMLKEYPGLSVNLVTGIPAPDLIADGLDVVIRVGALQDSSLFSRRLGA
                     MPMVVCAAKSYLTQYGIPEKPADLSSHSWLEYSVRPDNEFELIAPEGISTRLIPQGRF
                     VTNDPMTLVRWLTAGAGIAYVPLMWVINEINRGELEILLPRYQSDPRPVYALYTEKDK
                     LPLKVQVVINSLTDYFVEVGKLFQEMHGRGKEK"
     gene            complement(1072..2517)
                     /gene="tldD"
                     /locus_tag="ACHKBL_002082"
     CDS             complement(1072..2517)
                     /gene="tldD"
                     /locus_tag="ACHKBL_002082"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HFO5818959.1"
                     /translation="MSLNLVSEQLLAANGLKHQDLFAILGQLAERRLDYGDLYFQSSY
                     HESWVLEDRIIKDGSYNIDQGVGVRAISGEKTGFAYADQISLLALEQSAQAARTIVRD
                     SGDGKVQTLGAVEHSPLYTSVDPLQSMSREEKLDILRRVDKVAREADKRVQEVTASLS
                     GVYELILVAATDGTLAADVRPLVRLSVSVLVEEDGKRERGASGGGGRFGYEFFLADLD
                     GEVRADAWAKEAVRMALVNLSAVAAPAGTMPVVLGAGWPGVLLHEAVGHGLEGDFNRR
                     GTSVFSGQVGELVASELCTVVDDGTMVDRRGSVAIDDEGTPGQYNVLIENGILKGYMQ
                     DKLNARLMGMTPTGNGRRESYAHLPMPRMTNTYMLPGKSTPQEIIESVEYGIYAPNFG
                     GGQVDITSGKFVFSTSEAYLIENGKVTKPVKGATLIGSGIETMQQISMVGNDLKLDNG
                     VGVCGKEGQSLPVGVGQPTLKVDNLTVGGTA"
     gene            complement(2673..6473)
                     /gene="yhdP"
                     /locus_tag="ACHKBL_002083"
     CDS             complement(2673..6473)
                     /gene="yhdP"
                     /locus_tag="ACHKBL_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA2 domain-containing protein YhdP"
                     /protein_id="HFO5818960.1"
                     /translation="MRRLPGILLLTGAALVVIAALLVSGLRIALPHLDAWRPEILNKI
                     ESATGMPVEASQLSASWQNFGPTLEAHDIRAELKDGGEFSVKRVTLALDVWQSLLHMR
                     WQFRDLTFWQLRFRTNTPITSGGGNDSLEASHISDLFLRQFDHFDLRDSEVSFLTPSG
                     QRAELAIPQLTWLNDPRRHRAEGQVSLSSLTGQHGVMQVRMDLRDDEGLLSNGRVWLQ
                     ADDIDLKPWLGKWMQDNIALETAQFSLEGWMTIDKGDVTGGDVWLKQGGASWLGEKQT
                     HTLSVDNLTAHITRENPGWQFSIPDTRITMDGKPWPSGALTLAWIPEQDVGGKDNKRS
                     DELRIRASNLELAGLEGIRPLAAKLSPALGDVWRSTQPSGKINTLALDIPLQAADKTR
                     FQASWSDLAWKQWKLLPGAEHFSGTLSGSVENGLLTASMKQAKMPYETVFRAPLEIAD
                     GQATISWLNNDKGFQLDGRNIDVKAKAVHARGGFRYLQPANDEPWLGILAGISTDDGS
                     QAWRYFPENLMGKDLVDYLSGAIQGGEADNATLVYGGNPQLFPYKHNEGQFEVLVPLR
                     NAKFAFQPDWPALTNLDIELDFINDGLWMKTDGVNLGGVRASNLTAVIPDYSKEKLLI
                     DADIKGPGKAVGPYFDETPLKDSLGATLQELQLDGDVNARLHLDIPLNGELVTAKGDV
                     TLRNNSLFIKPLDSTLKNLSGKFSFINGDLQSEPLTASWFNQPLNVDFSTKEGAKAYQ
                     VAVNLNGNWQPAKTGVLPEAVNEALSGSVAWDGKVGIDLPYHAGATYNVELNGDLKNV
                     SSHLPSPLAKPAGEPLPVNVKVDGNLNSFELTGQAGADNHFNSRWLLGQKLTLDRAIW
                     AADSKTLPPLPEQSGVELNMPPMNGAEWLALFQKGAAESVGGAASFPQHITLRTPMLS
                     LGNQQWNNLSIVSQPTANGTLVEAQGREINATLAMRNNAPWLANIKYLYYNPSVAKTH
                     GDSTNSSPFPTTERISFRGWPDAQIRCAECWFWGQKFGRIDSDITISGNTLTLTNGLI
                     DTGFSRLTADGEWVNNPGNERTSLKGKLRGQKIDAAAEFFGVTTPIRQSSFNVDYDLH
                     WRKAPWQPDEATLNGIIHTQLGKGEITEINTGHAGQLLRLLSVDALMRKLRFDFRDTF
                     GEGFYFDSIRSTAWIKDGVMHTDDTLVDGLEADIAMKGSVNLVRRDLNMEAVVAPEIS
                     ATVGVAAAFAVNPIVGAAVFAASKVLGPLWSKVSILRYHISGPLDDPQINEVLRQPRK
                     EKAQ"
     gene            complement(6541..8010)
                     /gene="rng"
                     /locus_tag="ACHKBL_002084"
     CDS             complement(6541..8010)
                     /gene="rng"
                     /locus_tag="ACHKBL_002084"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HFO5818961.1"
                     /translation="MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKG
                     RVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVAGEEQKQFTVRDISELVRQGQD
                     LMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
                     YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQR
                     VLRDFADAELDRIRVDSRLTYEALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEI
                     QRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQ
                     LRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKR
                     TRESIEHVLCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAE
                     ALKGEESHSLAEVEIFVGKQVKVQIEPLYNQEQFDVVMM"
     gene            complement(8000..8593)
                     /gene="yhdE"
                     /locus_tag="ACHKBL_002085"
     CDS             complement(8000..8593)
                     /gene="yhdE"
                     /locus_tag="ACHKBL_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase YhdE"
                     /protein_id="HFO5818962.1"
                     /translation="MTSLYLASGSPRRQELLAQLGVTFERIVTGIEEQRQPQESAQQY
                     VVRLAREKAQAGVAQTAQDLPVLGADTIVILNGEVLEKPRDAEHAAQMLRKLSGQTHQ
                     VMTAVALADSQHILDCLVVTDVTFRTLTDEDIAGYVASGEPLDKAGAYGIQGLGGCFV
                     RKINGSYHAVVGLPLVETYELLSNFNALREKRDKHDG"
     gene            complement(8602..9090)
                     /gene="mreD"
                     /locus_tag="ACHKBL_002086"
     CDS             complement(8602..9090)
                     /gene="mreD"
                     /locus_tag="ACHKBL_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HFO5818963.1"
                     /translation="MASYRSQGRWVIWLSFLIALLLQIMPWPDNLIVFRPNWVLLILL
                     YWILALPHRVNVGTGFVMGAILDLISGSTLGVRVLAMSIIAYLVALKYQLFRNLALWQ
                     QALVVMLLSLVVDIIVFWAEFLVINVSFRPEVFWSSVVNGVLWPWIFLLMRKVRQQFA
                     VQ"
     gene            complement(9090..10193)
                     /gene="mreC"
                     /locus_tag="ACHKBL_002087"
     CDS             complement(9090..10193)
                     /gene="mreC"
                     /locus_tag="ACHKBL_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HFO5818964.1"
                     /translation="MKPIFSRGPSLQIRLILAVLVALGIIIADSRLGTFSQIRTYMDT
                     AVSPFYFVSNAPRELLDGVSQTLASRDQLELENRALRQELLLKNSELLMLGQYKQENA
                     RLRELLGSPLRQDEQKMVTQVISTVNDPYSDQVVIDKGSVNGVYEGQPVISDKGVVGQ
                     VVAVAKLTSRVLLICDATHALPIQVLRNDIRVIAAGNGCTDDLQLEHLPANTDIRVGD
                     VLVTSGLGGRFPEGYPVAVVSSVKLDTQRAYTVIQARPTAGLQRLRYLLLLWGADRNG
                     ANPMTPEEVHRVANERLMQMMPQVLPSPDAMGPKLPEPATGITQPTPQQPATGNAVTA
                     PAAPTQPAANRSPQRATPPQSGAQPPARAPGGQ"
     gene            complement(10259..11302)
                     /gene="mreB"
                     /locus_tag="ACHKBL_002088"
     CDS             complement(10259..11302)
                     /gene="mreB"
                     /locus_tag="ACHKBL_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HFO5818965.1"
                     /translation="MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQD
                     RAGSPKSVAAVGHDAKQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNS
                     FMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATG
                     SMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERI
                     KHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALE
                     QCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALE
                     MIDMHGGDLFSEE"
     gene            complement(11607..13547)
                     /gene="csrD"
                     /locus_tag="ACHKBL_002089"
     CDS             complement(11607..13547)
                     /gene="csrD"
                     /locus_tag="ACHKBL_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase E specificity factor CsrD"
                     /protein_id="HFO5818966.1"
                     /translation="MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQA
                     VATAIDTHLVSNDFSTLRPQITELMMSADIVRVDLLHGDKQVYTLARNGSYRPVGTND
                     LFRELSVPLIKHPGMSLRLVYQDPMGNYFHSLMTTAPLTGAIGFIILMLFLAVRWLQR
                     QLAGQELLETRATRILNGERGSNVLGTIYEWPPRTSSALDTLLCEIQNAREQHSRLDT
                     LIRSYAAQDMKTGLNNRLFFDNQLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHS
                     QVEEQFFSLTNLLSTFMMRYPGALLARYHRSDFAALLPHRTLKEAESIASQLIKAVDT
                     LPNNKMLDRDDMIHIGICAWRSGQDTEQVMEHAESATRNAGLQGGNSWAIYDDSLPEK
                     GRGNVRWRTLIEQMLSRGGPRLYQKPAVTREGRVHHRELMCRIFDGNEEVSSAEYMPM
                     VLQFGLSEEYDRLLISRLIPLLRYWPEENLAIQVTVESLIRPRFQRWLRDTLMQCEKS
                     QRRRIIIELAEADVGQHISRLQPVIRLVNALGVRVAVNQAGLTLVSTSWIKELNVELL
                     KLHPGLVRNIEKRTENQLLVQSLVEACSGTSTQVYATGVRSRSEWQTLIQRGVTGGQG
                     DFFASSQPLDTNVKKYSQRYSV"
     gene            13699..14673
                     /gene="yhdH"
                     /locus_tag="ACHKBL_002090"
     CDS             13699..14673
                     /gene="yhdH"
                     /locus_tag="ACHKBL_002090"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase (NAD+)
                     activity, zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family oxidoreductase"
                     /protein_id="HFO5818967.1"
                     /translation="MQALLLEQQDGKTLASVQTLDESRLPEGDVTVDVHWSSLNYKDA
                     LAITGKGKIIRNFPMIPGIDFAGTVRTSEDPRFHAGQEVLLTGWGVGENHWGGLAEQA
                     RVKGDWLVAMPQGLDARKAMIIGTAGFTAMLCVMALEDAGVRPQDGEVVVTGASGGVG
                     STAVTLLHKLGYQVVAVSGRESTHEYLKSLGASRILPRDEFAESRPLEKQVWAGAIDT
                     VGDKVLAKVLAQMNYGGCVAACGLAGGFTLPTTVMPFILRNVRLQGVDSVMTPPARRA
                     QAWQRLVADLPESFYTQAAKEISLAEAPKFAEAIINNQIQGRTLVKVN"
     gene            14859..15246
                     /locus_tag="ACHKBL_002091"
                     /pseudo
     CDS             14859..15246
                     /locus_tag="ACHKBL_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001240072.1"
                     /note="in Escherichia coli this inner membrane protein was
                     found to anchor the periplasmic catalytic oxidoreductase
                     YedY; sulfite oxidase activity not demonstrated; contains
                     heme; frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoxide reductase heme-binding subunit YedZ"
     gene            15651..16121
                     /gene="accB"
                     /locus_tag="ACHKBL_002092"
     CDS             15651..16121
                     /gene="accB"
                     /locus_tag="ACHKBL_002092"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HFO5818968.1"
                     /translation="MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVM
                     QQAYAAPMMQQPAQSNAAAPATVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAF
                     IEVGQKVNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE"
     gene            16132..17481
                     /gene="accC"
                     /locus_tag="ACHKBL_002093"
     CDS             16132..17481
                     /gene="accC"
                     /locus_tag="ACHKBL_002093"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HFO5818969.1"
                     /translation="MLDKIVIANRGEIALRILRACKELGIKTVAVHSSADRDLKHVLL
                     ADETVCIGPAPSVKSYLNIPAIISAAEITGAVAIHPGYGFLSENANFAEQVERSGFIF
                     IGPKAETIRLMGDKVSAIAAMKKAGVPCVPGSDGPLGDDMDKNRAIAKRIGYPVIIKA
                     SGGGGGRGMRVVRGDAELAQSISMTRAEAKAAFSNDMVYMEKYLENPRHVEIQVLADG
                     QGNAIYLAERDCSMQRRHQKVVEEAPAPGITPELRRYIGERCAKACVDIGYRGAGTFE
                     FLFENGEFYFIEMNTRIQVEHPVTEMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHA
                     VECRINAEDPNTFLPSPGKITRFHAPGGFGVRWESHIYAGYTVPPYYDSMIGKLICYG
                     ENRDVAIARMKNALQELIIDGIKTNVDLQIRIMNDENFQHGGTNIHYLEKKLGLQEK"
     gene            17590..17832
                     /gene="yhdT"
                     /locus_tag="ACHKBL_002094"
     CDS             17590..17832
                     /gene="yhdT"
                     /locus_tag="ACHKBL_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HFO5818970.1"
                     /translation="MDTRFVQAHKEARWALGLTLLYLAVWLVAAYLPGVAPGFTGFPR
                     WFEMACILTPLLFIGLCWAMVKFIYRDIPLEDDDAA"
     gene            17822..19273
                     /gene="panF"
                     /locus_tag="ACHKBL_002095"
     CDS             17822..19273
                     /gene="panF"
                     /locus_tag="ACHKBL_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015081 - sodium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015233 - pantothenate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015887 - pantothenate transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0036376 - sodium ion export across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HFO5818971.1"
                     /translation="MQLEVILPLVAYLVVVFGISVYAMRKRSTGTFLNEYFLGSRSMG
                     GIVLAMTLTATYISASSFIGGPGAAYKYGLGWVLLAMIQLPAVWLSLGILGKKFAILA
                     RRYNAVTLNDMLFARYQSRLLVWLASLSLLVAFIGAMTVQFIGGARLLETAAGIPYET
                     GLLIFGISIALYTAFGGFRASVLNDTMQGLVMLIGTIVLLIGVVHAAGGLSNAVETLQ
                     TIDPQLVTPQGADDILSPAFMTSFWVLVCFGVIGLPHTAVRCISYKDSKAVHRGIIIG
                     TIVVAILMFGMHLAGALGRTVIPDLTVPDLVIPTLMVKVLPPFAAGIFLAAPMAAIMS
                     TINAQLLQSSATIIKDLYLNIRPDQMQNETRLKRMSAVITLVLGALLLLAAWKPPEMI
                     IWLNLLAFGGLEAVFLWPLVLGLYWERANAKGALSAMIVGGVLYAVLATLNIQYLGFH
                     PIVPSLLLSLLAFLVGNRFGTSVPQATVLTTDK"
     gene            19285..20166
                     /gene="prmA"
                     /locus_tag="ACHKBL_002096"
     CDS             19285..20166
                     /gene="prmA"
                     /locus_tag="ACHKBL_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HFO5818972.1"
                     /translation="MPWIQLKLNTTGANAEDLSDALMEAGAVSITFQDTHDTPVFEPL
                     PGETRLWGDTDVIGLFDAETDMNDVVAILENHPLLGAGFAHKIEQLEDKDWEREWMDN
                     FHPMRFGERLWICPSWRDVPDENAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLTGK
                     TVIDFGCGSGILAIAALKLGAAKAIGIDIDPQAIQASRDNAERNGVSDRLELYLPKDQ
                     PEEMKADVVVANILAGPLRELAPLISVLPVSGGLLGLSGILASQAESVCEAYADSFAL
                     DPVVEKEEWCRITGRKN"
     gene            20495..21460
                     /gene="dusB"
                     /locus_tag="ACHKBL_002097"
     CDS             20495..21460
                     /gene="dusB"
                     /locus_tag="ACHKBL_002097"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HFO5818973.1"
                     /translation="MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSS
                     NPQVWESDKSRLRMVHIDEPGIRTVQIAGSDPKEMADAARINVESGAQIIDINMGCPA
                     KKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAED
                     CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGA
                     DALMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGY
                     RIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA"
     gene            21486..21782
                     /gene="fis"
                     /locus_tag="ACHKBL_002098"
     CDS             21486..21782
                     /gene="fis"
                     /locus_tag="ACHKBL_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HFO5818974.1"
                     /translation="MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNG
                     QDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN"
     gene            21868..22752
                     /gene="yhdJ"
                     /locus_tag="ACHKBL_002099"
     CDS             21868..22752
                     /gene="yhdJ"
                     /locus_tag="ACHKBL_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417728.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HFO5818975.1"
                     /translation="MRTGCEPTRFGNEAKTIIQGDALTELKKLPAESVDLIFADPPYN
                     IGKNFDGLIEAWKEDLFIDWLFEVIAECHRVLKKQGSMYIMNSTENMPFIDLQCRKLF
                     TIKSRIVWSYDSSGVQAKKHYGSMYEPILMMVKDAKNYTFNGDAVLVEAKTGSQRALI
                     DYRKNPPQPYNHQKVPGNVWDFPRVRYLMDEYENHPTQKPEALLKRIILASSNPGDIV
                     LDPFAGSFTTGAVAVANGRKFIGIEINSEYIKMGLRRLDVASHYSAEELAKVKKRKTG
                     NLSKRSRLSEVDPDLIAK"
     gene            22836..>22995
                     /locus_tag="ACHKBL_002100"
     CDS             22836..>22995
                     /locus_tag="ACHKBL_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2556 family protein"
                     /protein_id="HFO5818976.1"
                     /translation="MIRKYWWLVVFAVFVFLFDTLLMQWIELLATETDKCRNMNSVNP
                     LKLVNCDEL"
BASE COUNT         5517 a         6146 c         5831 g         5501 t
ORIGIN      
        1 agtcagagtg aataatggaa cgactaaaac gcatgtcggt gtttgccaaa gtagttgaat
       61 ttggctcttt taccgccgcc gccagacagc tacagatgag cgtttcgtcc atcagtcaga
      121 cggtgtcaaa actggaagat gagttacagg taaagctgtt aaaccgtagt acacgcagca
      181 ttggcctgac tgaagccggc agaatttact accagggctg ccgtcggatg ctccatgaag
      241 tgcaggatgt tcatgagcaa ctgtatgcct tcaataacac ccccatcggg acgctgcgca
      301 ttggctgttc ttcaactatg gcacaaaatg ttctcgccgg gctgacagca aaaatgctga
      361 aagaataccc aggtttgagc gtcaatctgg ttaccggaat tcctgccccc gacctgattg
      421 ccgatggtct ggatgtggtg atccgcgtcg gcgcgttgca ggattccagc ctgttttccc
      481 gccgtctggg cgcgatgccg atggtggtgt gcgccgcgaa aagctatctc acacaatacg
      541 gcataccgga aaaacccgcc gatttgagta gtcattcatg gcttgaatac agcgtgcggc
      601 ccgacaatga atttgaactg atcgcaccgg aagggatctc gactcgcctg atcccacaag
      661 gaagatttgt gactaatgat ccgatgacgc tggtgcgctg gctgacggcg ggtgccggga
      721 tcgcctacgt gccgctgatg tgggtgatca acgagatcaa tcgtggggaa ctggagatcc
      781 tgctgccgcg ttatcagtca gatccacgcc cggtttatgc gttatatacc gaaaaagata
      841 agctgccgct gaaggtacag gtcgtgatca actcgctgac ggattatttt gttgaggtcg
      901 gtaaattgtt tcaggagatg cacgggcgcg ggaaagagaa gtaattcata ttgtactgtt
      961 acgttgtaca aacctgtgcc aacgggttcc cctcacccta accccagagg agcgagggga
     1021 ccgttcgtgc acgtagaaag attaattatc cttctgaaaa tactgaaatt attacgcagt
     1081 accgccaaca gtcaggttat cgactttcaa cgttggctgg cccacgccaa ccggcaaact
     1141 ttgcccttct ttaccgcaga cacccacgcc gttatccagt ttcaggtcgt tacccaccat
     1201 cgaaatctgc tgcatggttt cgataccgga accaatcaac gttgcgcctt tcaccggctt
     1261 cgttacttta ccgttttcaa tcagatatgc ttctgaagtg gagaaaacga atttgccgga
     1321 ggtgatatcc acctgaccgc caccaaagtt cggtgcatag ataccgtact caacggattc
     1381 aataatttcc tgcggggtcg atttacccgg cagcatatag gtgttggtca tacgcggcat
     1441 gggcagatgg gcgtaggatt cacggcgacc gttgccagtc ggcgtcatcc ccatcaaacg
     1501 cgcgttgagt ttatcctgca tgtagccttt caggatgcca ttctcaatca gcacgttgta
     1561 ctggcctggc gtaccttcgt catcaatcgc caccgaacca cggcgatcga ccatcgtacc
     1621 gtcatcaacc acggtacaca gttctgaagc caccagctcc ccaacctgtc cactaaatac
     1681 tgaagtgcca cggcggttga agtcgccttc cagaccgtga cctaccgctt catgcaacag
     1741 cacgcccggc caacctgcgc caagtactac cggcatggtg cccgctggtg cagcaacggc
     1801 agaaagattg accagcgcca tacgcactgc ttcttttgcc catgcatccg cacggacttc
     1861 gccgtcgaga tcggcaagga agaactcata accaaaacga ccgccgccgc cactggcacc
     1921 gcgttcgcgt ttgccatctt cttcgaccag aacgctcacg gaaagacgca ccagcggacg
     1981 gacatccgcc gctagcgtgc cgtcggtggc cgcaaccaga attaattcat agacaccact
     2041 gaggctggca gtcacttcct gtacgcgctt gtccgcttcg cgggcaacct tatcgacgcg
     2101 acgcaggata tccagcttct cttcacggct catgctttgc agcggatcta ccgaggtata
     2161 caacgggcta tgctctaccg cgcccagcgt ctgtacttta ccatcaccac tatcacggac
     2221 gatggtgcgc gccgcttgtg cactctgttc cagcgccagc aggctgattt ggtcagcata
     2281 agcaaatccg gttttttcac cactgattgc acgcacgcca acgccctgat cgatgttgta
     2341 agaaccatct ttaataatac ggtcttctaa aacccaggat tcgtgatagc tcgactgaaa
     2401 atagagatcg ccataatcaa ggcgacgttc ggccagttga ccgaggatcg cgaacaagtc
     2461 ctgatgtttc aggccgttcg ccgctagcaa ttgttcactt accaggttca gactcatcgt
     2521 ttttgctact cgttagttac tgcagtagag gattttttac ggctgccgga tgcggcgtga
     2581 acgccctgtc cggcctacgg ttatgttctg gctcgttggc ctttggcaac gattatccta
     2641 tgagattggg gcaattacgc gccctcgtca aatcattgcg ctttttcttt acgcggttgt
     2701 cgcaacactt cgttgatttg cggatcgtcc agcggacccg aaatgtgata gcgcaaaatg
     2761 gagactttgc tccacagcgg tcccagcact ttactggcgg caaacactgc cgcgccaaca
     2821 atggggttaa ccgcaaaggc cgcagccaca cccaccgtcg cagaaatctc tggtgcgaca
     2881 accgcttcca tattcaggtc gcgacgtacc agatttaccg accctttcat agcgatgtcc
     2941 gcttccaggc catccaccag cgtgtcgtcg gtgtgcataa cgccgtcttt aatccacgcg
     3001 gtgctgcgaa tggagtcaaa atagaaccct tcgccaaaag tgtctctgaa atcaaaacgc
     3061 agcttacgca tcagggcatc tacgctcaat aagcgcagca attgcccggc atgtccggta
     3121 ttgatttcgg taatttcgcc tttacccagt tgagtatgaa tgatgccatt caacgtcgcc
     3181 tcatctggtt gccagggtgc tttgcgccag tgtaaatcgt aatccacatt aaatgacgac
     3241 tggcgtatgg gcgtcgtgac accaaaaaat tctgcggcgg catcaatttt ctgcccacgc
     3301 agttttcctt tcagcgaggt gcgttcattc cccggattgt taacccattc accatcggca
     3361 gtgagccgcg aaaaaccagt atcaatcagt ccattagtca gcgttaatgt attgccagaa
     3421 atggtgatat cactgtcaat gcgcccgaat ttttgccccc agaaccagca ctctgcgcat
     3481 cgtatttgtg cgtccggcca gccacggaag ctaatgcgct ccgttgtcgg gaaaggtgat
     3541 gaattcgttg agtcaccatg agttttcgcc acgcttgggt tgtaataaag atatttgata
     3601 ttcgccagcc acggcgcgtt attacgcatc gccagcgtgg cgttgatttc acgcccttgc
     3661 gcctcaacca gggtgccatt tgccgtcggt tgcgaaacaa tgctcaggtt attccattgc
     3721 tgatttccca gcgacaacat aggcgtacgt aacgttatgt gttgtgggaa actcgctgca
     3781 ccaccgacac tctccgcagc gcctttctgg aacagggcca gccactcggc accattcatc
     3841 ggcggcatat tgagttcaac gccactttgt tccggcaacg gcgggagcgt tttactgtct
     3901 gccgcccaaa tagcacggtc gagcgtcagc ttttgaccga gcaaccagcg gctattgaaa
     3961 tggttatctg caccagcctg tccggttaac tcaaagctgt tgagattgcc atcaacctta
     4021 acgtttaccg gcagtggttc acccgcaggt ttggctaacg gtgaaggtaa gtgactgctc
     4081 acattcttca ggtcgccgtt cagctctacg ttataggtcg cgccagcatg ataaggcaga
     4141 tcaatgccca ctttaccatc ccacgccacg ctgccactta atgcttcgtt caccgcttca
     4201 ggcagaacgc cggttttcgc tggttgccag ttaccgttga gattcaccgc gacctggtag
     4261 gcttttgccc cttctttggt ggaaaaatcc acgttcaacg gctgattaaa ccagcttgct
     4321 gtcagtggtt cactttgcag atcgccattg ataaagctga atttaccgct caaatttttc
     4381 agggtgctgt cgagtggttt gataaacaga ctgttattac gcagcgtcac atcacctttc
     4441 gcggttacca gttcgccgtt cagcgggata tcaagatgta agcgagcatt cacatcgccg
     4501 tcgagctgga gttcttgcag ggtcgcaccc agagaatctt tcagcggtgt ctcatcaaag
     4561 taagggccaa cggctttacc cggaccttta atgtcagcgt caatcagcag tttttctttt
     4621 gagtagtcag ggatcactgc ggtgagatta cttgcgcgca cgccgcccag attaacgcca
     4681 tcggttttca tccataaacc gtcgttaata aagtccagtt caatatcaag gttagttaat
     4741 gcaggccagt ccggctggaa ggcaaacttc gcgttgcgca gcggtaccag cacttcaaac
     4801 tgaccttcgt tgtgtttata ggggaagagt tgcggattgc caccataaac cagcgtcgcg
     4861 ttatccgctt caccgccctg aatcgcgcca cttaagtaat caaccaggtc tttacccatc
     4921 aagttttccg ggaaatagcg ccaggcttgt gaaccatcat cggtactgat gccagccaga
     4981 atacccagcc agggttcatc gttagctggt tgcaggtaac gaaaaccgcc gcgcgcatgg
     5041 acggctttgg ctttaacgtc aatattacgc ccatccagct ggaaaccttt gtcattgttc
     5101 agccagctta tcgttgcctg gccgtcggcg atttccagtg gcgcacggaa taccgtttcg
     5161 taaggcatct ttgcctgctt catcgacgca gtaagcaaac cattttctac gctgccggaa
     5221 agcgtcccgg agaagtgttc cgcacccggt aataatttcc attgcttcca ggccagatcg
     5281 ctccacgatg cctgaaaacg ggtcttgtct gccgcctgaa gcgggatatc cagcgccagc
     5341 gtgttaatct tgccgctcgg ttgtgtggag cgccaaacat cacccagtgc aggtgaaagt
     5401 ttcgcggcca gcgggcgtat gccctccagg cctgccagct ccagattact ggcgcgaatc
     5461 cggagttcgt cactgcgttt attgtctttg ccgccaacgt cctgttccgg tatccaggcc
     5521 agcgtcaatg ctccgctcgg ccagggtttg ccgtccatcg tgatccgtgt atcgggaata
     5581 gagaactgcc agcctggatt ttcacgcgta atatgcgcgg tcagattatc caccgacagc
     5641 gtatgcgttt gcttctcgcc caaccagctg gcaccgccct gtttcagcca gacgtcaccg
     5701 ccggttacat cgcctttgtc gatcgtcatc cagccttcaa gggagaactg tgccgtttcc
     5761 agcgcaatat tgtcctgcat ccatttaccg agccacggct tcaggtcgat gtcatccgcc
     5821 tggagccaga cgcgaccatt gcttaacaac ccctcatcat cgcgcaaatc catgcgcacc
     5881 tgcatcacgc cgtgctgtcc ggtaaggctg gagaggctta cctggccttc tgcacggtgt
     5941 cgacgtggat cgttcagcca ggtgagctgt gggatcgcca gctcggcgcg ctgaccggat
     6001 ggcgtcagga aactgacttc actgtcgcgg agatcgaaat ggtcgaactg acgaagaaac
     6061 agatcgctga tgtgactggc ttccagacta tcattaccac cgccgctggt gataggagtg
     6121 ttggtgcgaa agcgcagctg ccagaaagtg aggtcgcgaa actgccagcg catatgtaac
     6181 aggctctgcc agacatccag cgccagagta acgcgtttaa ccgaaaattc gccgccatct
     6241 tttagttctg cacggatgtc gtgcgcttca agcgtcggac caaaattctg ccagctggct
     6301 gagagctgac tggcttctac cggcatgcca gtcgcggatt ctattttgtt gaggatttcc
     6361 ggacgccagg cgtcaagatg cggtaaagca atacgcaggc cgctaaccag cagggcagcg
     6421 atcacaacga gcgcggctcc agtaagcagt aaaatccccg gcaatcgcct cacccgtcac
     6481 tccttgtctg ctaaaaatgt gactcaaaaa ccctttgccg gatggcggcc cagcatctgt
     6541 ttacatcatt acgacgtcaa actgctcctg gttatagagc ggttcaattt gtactttaac
     6601 ctgtttacca acgaaaattt ccacttccgc cagcgagtgt gactcttcgc ctttcaaggc
     6661 ttcagctact gccggagaag catagaccag gaaacggtcg gaatcataag catggtgtac
     6721 acgaacaatc tcgcgcatga tttcatagca taccgtttcc acggttttca ccgttccgcg
     6781 accgtggcag gttgggcatt cgttacatag tacgtgctca atgctttcgc gggtgcgttt
     6841 acgcgtcatc tccaccagcc ccagcgccga aaaaccatta acgctggttt tcacccggtc
     6901 tttgctcaac gcctgctcca gcgagtgcag cacgcggcgg cggtgatctt cattattcat
     6961 atcgatgaaa tcaataatga taatcccgcc cagattacgc aaccgtaact ggcgagcgat
     7021 agcctgcgtc gcttcaatat tggtattgaa aatggtgtcg tccagattgc gatgaccgac
     7081 aaacgccccg gtattgatgt ctacggtggt catcgcttcg gtctggtcga taatcagata
     7141 gccaccggat ttcagttcta ctttgcgttc cagcgctcgc tgaatttcgt tttcgacatc
     7201 aaagagatcg aaaatcggct ggcgtccggt gtaatgctcc agcttgctgg tcatctcggg
     7261 aatgtactcc gaggtaaact ccagcaatgc ttcgtaagtc aggcgtgagt caacgcgaat
     7321 gcggtcgagt tcggcgtcgg cgaaatcacg cagaacacgc tgcgccagcg ccagttcgcc
     7381 gtacagctga taacgggtct gtgggcgctt tttacgctcc ataactttgg tccagacgcg
     7441 tttcagataa gcggcatcgg aggccagttc agcctcgcca accccttcag ctgcggtacg
     7501 gatgataaac ccgccctgct cgtcgcaata ctctgcgacc acttttttca ggcgttcacg
     7561 ttctgattcg ctttcaattc gttgggaaac cccaacgtga gaagcccctg gcataaatac
     7621 cagatagcga gaaggcagcg tgatatcggt ggtcaggcgc gcacccttag tgccaagtgg
     7681 atctttcacc acctgcacca tcagatcttg cccctgacga accagttccg agatgtcgcg
     7741 caccgtgaat tgcttttgtt cttcacccgc cacacattcg gtgtgcggca tgatgtcaga
     7801 tgcatgaaga aacgcggctt tatccagccc aatatctaca aaagccgcct gcatacccgg
     7861 aagtacacga cttacacgac ccttgtagat attgcctact attccgcgtc gcgcctcacg
     7921 ttcaatatga atttcctgca gaataccgcc atcaatatac gccactcgcg tttccgaagg
     7981 cgttacgttt actaacaatt cagccgtcat gtttatccct tttctcacgc agtgcgttaa
     8041 aattacttaa taattcatac gtttcaacca gcggtaagcc gactacggcg tgatagctgc
     8101 catttatctt cctgacaaaa cagccaccca gcccctgaat accgtatgca cctgctttat
     8161 ctaacggttc accgctggcg acatagcccg cgatgtcttc gtctgttaac gttctgaaag
     8221 tcacatcggt gaccaccagg caatcgagaa tgtgctggct gtcggccaac gccactgccg
     8281 tcattacctg atgggtctga cccgataatt tgcgcaacat ctgcgccgca tgctctgcgt
     8341 cgcgcggttt ctccagtact tctccgttca ggataacgat agtatccgca cccagcaccg
     8401 ggagatcctg cgccgtttgc gcgacacccg cctgtgcttt ctcgcgcgcc agacgcacaa
     8461 catactgctg cgcgctctcc tgcggctgac gctgctcctc aatgcccgta acaatacgtt
     8521 caaaggttac gccaagttgc gcaagtaact cctgacgacg cggagaaccg gaagctaaat
     8581 acagagaagt catagaaacc tttattgcac tgcaaactgc tgacggactt tacgcatcag
     8641 caagaaaatc cacggccaga gcaccccatt gactacgcta ctccagaaca cttccggtct
     8701 gaaagagacg ttaatcacta aaaactctgc ccagaaaaca ataatatcca ccaccagcga
     8761 aagcaacatg acgaccagcg cctgctgcca taatgcgagg ttgcggaaaa gctggtattt
     8821 cagcgccacc aggtaagcaa tgatgctcat cgccaatacg cgtacgccaa gcgtcgagcc
     8881 gctgatcaga tccagtatgg cacccatcac aaaacctgtg cccacattta cgcgatgagg
     8941 caaggccagg atccaataca acaagatgag taacacccag tttggccgga aaacaatcag
     9001 gttatccggc cagggcatga tttgcagcaa cagcgcaatg aggaaagaga gccagattac
     9061 ccagcgtccc tggctacgat agctcgccac tattgccctc ccggcgcacg cgcaggcggt
     9121 tgagcaccac tttgcggcgg cgtagccctt tgtggagagc gattagcagc gggctgtgtg
     9181 ggcgcagcag gcgcagtaac tgcatttcct gtcgccggtt gctgcggagt cggctgagtg
     9241 atccccgttg ccggttcagg taactttggc cccatcgcgt ctggcgaagg caatacctgc
     9301 ggcatcatct gcatcaaacg ttcattagca acacgatgca cctcttccgg cgtcatcggg
     9361 ttagcgccgt tacgatccgc gccccacagc agcagcagat aacgcaaacg ttgcagtcct
     9421 gcagtcggac gcgcctgaat cacagtataa gcgcgctggg tatcgagttt tacggaagag
     9481 acaaccgcga ccggatagcc ttccgggaaa cgaccgccca gaccggaagt caccagcaca
     9541 tcaccaacac gaatgtccgt attcgctggc agatgctcaa gctgcaaatc atccgtacaa
     9601 ccgttaccgg ctgcaattac gcggatatcg ttgcgcagca cctggattgg cagcgcgtgg
     9661 gtcgcatcac aaatcagcag cacgcggctg gtcagtttag cgacggccac cacctgacca
     9721 acaacacctt tgtcgctgat aaccggctgg ccttcataaa cgccattaac gctaccttta
     9781 tcgataacaa cttgatcgct ataaggatcg ttaaccgtgg agataacctg agtcaccatt
     9841 ttctgctcat cctgacgcag cggggaaccc agtagctcgc gcagacgcgc gttctcctgt
     9901 ttgtattgtc caagcatcag cagttcactg tttttcagca acagttcctg acgtaacgcc
     9961 cggttttcaa gttctagttg gtcacgcgag gccagcgtct gcgatacgcc atccagcaat
    10021 tcacgaggag cattggaaac aaagtagaaa ggactgacgg cggtatccat ataagtacgg
    10081 atttgactga acgtccccag gcggctgtcg gcaataataa tgccgagcgc caccagcacc
    10141 gccagaataa ggcgaatctg tagcgacggg ccacggctaa aaattggctt cataagttat
    10201 gcgtattctc gtatcagacc aggcagggta aacagacact tcccctgcct gcatccgatt
    10261 actcttcgct gaacaggtcg ccgccgtgca tgtcgatcat ttccagcgct ttgccgccac
    10321 cgcgcgccac acaggtcagc gggtcttcag caacaacgac tggaatgccg gtttcttcca
    10381 ttaacaaacg gtcaaggtta cgcagcagtg cgccaccacc ggtaagcacc atgccacgct
    10441 cggagatgtc ggaagccagt tccggcgggc actgttccag tgcaaccatt accgcgctca
    10501 caataccggt cagcggttcc tgcagtgctt cgaggatttc gttggagttc agggtaaagc
    10561 cgcgtggaac accttctgca aggttacggc cacgaacttc gatttcacgt acttcatcgc
    10621 ccgggtaagc cgaaccgatt tcgtgcttga tacgttctgc ggtggcttca ccgatcagag
    10681 aaccgtaatt acgacgtaca tagttgatga tagcttcgtc gaaacggtca ccaccaatgc
    10741 gcacggaaga ggagtaaacc acgccgttca aggagataac agcaacttca gtagtaccac
    10801 caccgatatc aaccaccata gaaccggtcg cttcagaaac cggcaggcca gcaccaattg
    10861 cggcagccat cggttcttca atcaggaaga cttcacgggc accagcgccc tgcgcggatt
    10921 cacgaattgc gcggcgttca acctgggtcg cgccaaccgg cacacaaacc agaacgcgcg
    10981 ggcttggacg cataaagctg ttgctgtgca cttgtttgat gaagtgctgg agcatttttt
    11041 cagtcacgaa gaagtcggcg ataacgccgt ctttcattgg gcgaatagca gcaatattgc
    11101 ccggcgtacg gcccagcatc tgcttcgcgt catgacctac tgcagctacg cttttcggtg
    11161 aaccggcacg atcctgacga atggccacca cggaaggctc attcaatacg atgccttgtc
    11221 cttttacata aatgagggta ttcgcagtac ccaggtcaat ggacaagtca ttggaaaaca
    11281 tgccacgaaa ttttttcaac atactaaggg ataatcctga aagctggggc ggaaaagaaa
    11341 atccgcttac tttaccaacc acacgcagca gcgacaaggc gcaaaaatca tctgctacgg
    11401 tgaaaattag tgcagttcgt ttcctttgtt acaaatctct gcctgagtcc agaaaggctt
    11461 aatgcatcag cagcattcct cgcctgtttg caaccgcgta aggtcattca tctgcatatg
    11521 tgctgcaaca atctggcgag cagacaagca cactcccatg agacgcagcg cgcgttattc
    11581 tacgtgaaaa cggattaaac ggcaggttaa accgagtatc tttgtgaata ttttttcacg
    11641 ttagtatcaa gtggctgtga ggacgcgaaa aaatcccctt gcccacctgt aacaccgcgc
    11701 tgaatcaggg tctgccactc gcttcgcgaa cgcacgccgg tggcgtaaac ctgggtgctg
    11761 gtcccggagc aggcttccac caggctttga accagcagct gattctccgt tcgcttctca
    11821 atgtttctga ccagccccgg atggagcttg agtaactcaa cattaagttc tttgatccaa
    11881 ctggtgctta ccagcgtcaa accagcctgg ttgacggcta cccgtacccc taaagcattc
    11941 actaaacgaa taacaggttg taagcgactg atatgttgac caacatcggc ctctgcaagt
    12001 tcaataatta tgcgtcttcg ttgcgatttt tcacattgca ttaacgtatc gcgcagccaa
    12061 cgctgaaaac gcgggcgaat cagcgactca acggtaacct gaatcgccag attttcctct
    12121 ggccagtaac gcaatagtgg aataagacgg ctgattagca ggcggtcata ctcttccgat
    12181 aaaccaaact gcaagaccat cggcatatac tctgccgagc taacctcttc attaccatcg
    12241 aagatgcggc acatgagttc acgatgatga acccggcctt cgcgagtaac cgccggtttt
    12301 tgataaaggc gcgggccgcc gcgactgagc atttgctcga taagcgtacg ccagcgaaca
    12361 ttaccgcgtc ctttttcagg caacgagtca tcgtaaatag cccagctatt gccgccctgc
    12421 aatcccgcat tacgcgtggc agactctgca tgttccatta cctgctcggt atcctgacca
    12481 ctacgccagg cacagatacc aatgtggatc atatcgtcgc gatcgagcat tttattgttc
    12541 ggcaaggtat cgacggcttt gattaactga ctggcgatgc tctctgcctc ttttaacgtc
    12601 cggtgcggta acagcgcagc aaaatcactg cggtggtaac gcgccagcag tgcgccaggg
    12661 tagcgcatca taaatgtcga tagcagattc gtcagagaga agaactgttc ttcaacctgg
    12721 ctgtgccccc aggtatcact caacatattg aaatccggca gacgaatcat catcacgatc
    12781 ccgtgggtac ctactttctc ttgatcttcc agtaacgttg ctaactgatt atcgaaaaag
    12841 agtcggttat tgaggccggt tttcatgtcc tgggcggcat aagagcggat cagcgtatca
    12901 agacggctgt gttgttcgcg tgcgttctga atttcacaaa gcagcgtatc cagcgcactg
    12961 ctggttctgg gcggccattc atagatggtt cccaacacat tagagccacg ctcaccgttt
    13021 aagatacgag tagcccgggt ttccagcaat tcttgcccgg caagttgccg ttgtaaccag
    13081 cgtaccgcca ggaagagcat aagaatgata aagccaatcg cccccgtgag cggcgcggta
    13141 gtcattaaag aatggaaata gttgcccatc ggatcctgat aaaccagacg cagcgacatc
    13201 cccggatgct ttatcaacgg aacgctcagt tcacgaaaca gatcgttggt gccaaccgga
    13261 cgataactac catttctggc cagggtataa acctgcttat caccatggag caggtctaca
    13321 cgaacgatat ctgccgacat cattaattcg gtaatttgtg gccttaatgt gctgaagtca
    13381 ttcgacacta ggtgggtatc aatcgccgtc gccaccgcct gaacacgatg actaaactta
    13441 tactgaatgg cgttgtagaa acttagcgaa cagcccagca aagtcacaaa aattgttaac
    13501 ccggtgagca gcgtgacaaa ggccgaaaat ttcgtcgtta atctcatcct tgtgttaact
    13561 ccgatagtga ggaagcgggc atactagcaa atcagattta tctcgcaatt tattgcgctt
    13621 catcggcttt gcttttccat tagcgagtat agtcttcaga aattattttc caatccatca
    13681 tgcacatgag gaccacttat gcaggcgtta cttttagaac agcaggacgg caaaactctc
    13741 gcatcagtac agactctgga cgaaagtcgc ctgccggaag gcgatgtcac ggttgatgtt
    13801 cactggtcga gcctgaacta caaagatgcg ctggcgatta ccggtaaggg aaaaatcatc
    13861 cgaaattttc cgatgattcc tgggattgat tttgccggaa ctgtacgcac cagcgaagat
    13921 ccgcgttttc atgccggtca ggaggtgtta ctcactggct ggggcgttgg tgaaaaccac
    13981 tggggtgggc tggcggaaca ggcgcgagtg aaaggtgact ggctggttgc catgccgcaa
    14041 gggctggacg cgcgtaaagc aatgattatc ggtactgccg gttttaccgc catgctgtgt
    14101 gtgatggcgc tggaagatgc cggtgttcgc ccgcaggatg gggaggttgt cgtgacgggt
    14161 gccagtggtg gcgtcggcag taccgccgtc acgctgctgc ataagttggg ttatcaggtc
    14221 gttgccgttt ccggtcgcga aagtacccat gaatatctga aaagtttagg tgccagccgt
    14281 attcttcctc gcgatgagtt tgctgaatcc cgtcctctgg aaaaacaagt ctgggctggg
    14341 gcaattgaca ccgttggcga caaagtgctg gcaaaagtgc tggcgcaaat gaattacggc
    14401 ggctgcgtgg cggcctgtgg tctggcgggt ggttttactc tgccaaccac ggtcatgcca
    14461 tttattctgc gtaatgtccg tttgcaaggg gtggattcag taatgacgcc accagcacgc
    14521 cgcgcacaag cctggcagcg actggtcgcc gatttaccgg aatcattcta tacccaagcg
    14581 gcaaaagaga tatctctggc agaggcaccg aagtttgccg aggccatcat taataaccag
    14641 atccagggtc gcacgctggt gaaggttaac taaccattta gcagggaata ataagagagg
    14701 gaactcattt tgaaatcatt attcaatcgg ttaacgggaa aagcggttag ccggacagct
    14761 ttcgtcgaac atcttggtca tgaagttgta caacatcatc caaactggaa agtcatgatt
    14821 tcgactgacc ataaattgat gcgcattgat gctccaccaa acagccatta ttgatacgcc
    14881 tccgtcgcct gttaggttta tggtgttttg ccttggcgac gctacgctta atctgttact
    14941 gatttctggt accatggggg gaataatctg attttatttg attgcaaatt aaccacttaa
    15001 acctattgaa tgcggaacat tgtcagtcgg ttaattttgc gtgctttagc attcacatct
    15061 ttccggacga tgcagtgaaa attgcgtaat cctcagcaac cgctgcgtaa tgtcgtctat
    15121 cttgtcgcga tcctggcatc cctacattat ttgtggtctg tgaagattat ctcattgcag
    15181 cccctcatct tcgcagggct ggctttacag cttttcaccc tacgttataa gaagttccgt
    15241 cgatgatggc gctaatttcg tgaattgtgc ggcttgttgc aaattacacg gtgttgaagg
    15301 ttatttacat gttagctgtt gattatcttc cctgataaga ccagtattta gctgccaatt
    15361 gctacgaaat cgttataatg tgcgacctcg tcctccctga cgcagttttt gcgctgcgga
    15421 aaaggtgaca ttggcgcaac gaaggtatat tttgtttttt gccggaggat agcagcagat
    15481 cgctgcacaa tgtccgtcaa gtctaacatt gacactctgg ggcaaaatag accggcgtcc
    15541 cggcctgctg gaatttatcg ctatgcatac agctgtcggg gcatacgctt tacagacggc
    15601 ggtgaaacgc ctgtcacaat cacactaaac aaagagtacg gaacccactc atggatattc
    15661 gtaagattaa aaaactgatc gagctggttg aagaatcagg catctccgaa ctggaaattt
    15721 ctgaaggcga agagtcagta cgcattagcc gtgcagctcc tgccgcaagt ttccctgtga
    15781 tgcaacaagc ttacgctgca ccaatgatgc agcagccagc tcaatctaac gcagccgctc
    15841 cggcgaccgt tccttccatg gaagcgccag cagcagcgga aatcagtggt cacatcgtac
    15901 gttccccgat ggttggtact ttctaccgca ccccaagccc ggacgcaaaa gcgttcatcg
    15961 aagtgggtca gaaagtcaac gtgggcgata ccctgtgcat cgttgaagcc atgaaaatga
    16021 tgaaccagat cgaagcggac aaatccggta ccgtgaaagc aattctggtc gaaagtggac
    16081 aaccggtaga atttgacgag ccgctggtcg tcatcgagta acgaggcgaa catgctggat
    16141 aaaattgtta ttgccaaccg cggcgagatt gcattgcgta ttcttcgtgc ctgtaaagaa
    16201 ctgggcatca agactgtcgc tgtgcactcc agcgcggatc gcgatctaaa acacgtatta
    16261 ctggcagatg aaacggtctg tatcggccct gctccgtcag taaaaagtta tctgaacatc
    16321 ccggcaatca tcagcgccgc tgaaatcacc ggcgcagtag caatccatcc gggttacggc
    16381 ttcctctccg agaacgccaa ctttgccgag caggttgaac gctccggctt tatcttcatc
    16441 ggcccgaaag cagaaaccat tcgcctgatg ggcgacaaag tatccgcaat cgcggcgatg
    16501 aaaaaagcgg gcgttccttg cgtaccgggt tctgacggcc cgctgggcga cgatatggat
    16561 aaaaaccgcg ccattgctaa acgcattggt tatccggtaa ttatcaaagc ctccggcggc
    16621 ggcggcggtc gcggtatgcg cgtagtgcgc ggcgacgctg aactggcaca atccatctcc
    16681 atgacccgtg cggaagcgaa agctgctttc agcaacgata tggtttacat ggagaaatac
    16741 ctggaaaatc ctcgccacgt cgagattcag gtactggctg acggtcaggg caacgctatc
    16801 tatctggcgg aacgtgactg ctccatgcag cgccgccacc agaaagtggt cgaagaagca
    16861 ccagcaccgg gcattacccc ggaactgcgt cgctacatcg gcgaacgttg cgctaaagca
    16921 tgtgttgata tcggctatcg cggtgcgggt actttcgagt tcctgttcga aaacggcgag
    16981 ttctatttca tcgaaatgaa cacccgtatt caggtagaac acccggttac tgaaatgatc
    17041 accggcgttg acctgatcaa agaacagctg cgtatcgctg ccggtcaacc gctgtcgatc
    17101 aagcaagaag aagttcacgt tcgcggccat gcggtggaat gccgtatcaa cgccgaagat
    17161 ccgaacacct tcctgccaag cccgggcaaa atcacccgtt tccacgcacc aggcggtttt
    17221 ggcgtgcgtt gggagtctca tatctacgcg ggctacaccg taccgccgta ctatgactca
    17281 atgatcggta agctgatttg ctacggtgaa aaccgtgacg tggcgattgc ccgcatgaag
    17341 aatgcgctgc aggagctgat catcgacggt atcaaaacca acgttgatct gcagatccgc
    17401 atcatgaatg acgagaactt ccagcatggt ggcactaaca tccactatct ggagaaaaaa
    17461 ctcggtcttc aggaaaaata agactgcaaa agcgtcaaaa ggccggattt tccggccttt
    17521 tttattactg gggatcgaca acccccataa ggtacaatcc ccgctttctt cacccatcag
    17581 ggacaaaaaa tggacactcg ttttgttcag gcccataaag aggcgcgctg ggcgctgggg
    17641 ctgacccttt tgtatctggc ggtatggtta gtagccgctt acttacctgg cgttgccccc
    17701 ggttttaccg gttttccgcg ctggtttgag atggcctgca tcctgacgcc gctgctgttt
    17761 attggactgt gctgggcgat ggtgaaattt atctatcgcg atatcccact ggaggatgac
    17821 gatgcagctt gaagtaattc taccgctggt cgcctatctg gtggtggtgt tcggtatctc
    17881 tgtttatgcg atgcgtaaac ggagcactgg caccttcctt aatgagtatt tcctcggcag
    17941 ccgctctatg ggcggtattg tgctggcgat gacgctcacc gcgacctata tcagtgccag
    18001 ttcgtttatc ggcgggcctg gagctgctta taaatacggc ctgggctggg tattgctggc
    18061 gatgattcag cttcctgcag tctggctttc gctcggtatt ctcggcaaga agttcgcaat
    18121 tcttgcgcgc cgctacaatg cggtgacact gaacgacatg ctgtttgccc gctaccagag
    18181 tcgtcttttg gtgtggctgg cgagtttgag tttgctggtc gcgtttattg gcgcgatgac
    18241 cgtgcagttt atcggcggcg cgcgcctgct ggaaaccgcg gcgggtattc cttatgaaac
    18301 cgggctgctg attttcggta tcagcattgc gttgtatacc gcctttggtg gctttcgcgc
    18361 cagcgtgctg aacgacacta tgcaagggct ggtgatgctg attggcacca ttgtgctgct
    18421 tattggcgtg gtacatgccg caggtggctt aagcaacgca gtagagacgt tgcaaaccat
    18481 cgatccgcaa ctggttacgc cacaaggcgc tgacgatatt ctgtcgcctg cctttatgac
    18541 gtcgttctgg gtactggtgt gctttggcgt gattggcctg ccgcatactg cggtgcgctg
    18601 tatctcttat aaagacagca aagccgtaca tcgggggatc atcatcggta cgattgtggt
    18661 cgcaatcctg atgttcggta tgcacctggc cggagcgtta ggtcggacgg tgatccccga
    18721 tctcaccgta ccggacctgg tgatcccgac gttaatggta aaagtgttgc caccgtttgc
    18781 tgccgggatc ttcctggctg cacctatggc tgcgatcatg tcgacaatta acgcccaact
    18841 gctgcaaagt tccgctacga tcattaaaga tctctatctg aatatccgtc cggatcaaat
    18901 gcaaaacgag acgcgtctga agcggatgtc ggcggtaatt acgttagttc tcggtgcgtt
    18961 gctgctgctt gccgcctgga agccgccaga aatgatcatc tggctgaatt tgctggcctt
    19021 cggtgggctg gaagccgttt tcctgtggcc gctggtactg ggtctttact gggaacgcgc
    19081 caacgccaaa ggcgcgctaa gtgcgatgat tgttggcggc gtactgtatg ccgtactcgc
    19141 gacgctgaat attcagtacc tgggcttcca ccctatcgtg ccctcgttac tactaagttt
    19201 gctggctttc ctggtcggaa accgtttcgg tacatccgtc ccgcaagcta ccgttttgac
    19261 tactgataaa taaagagttt tgccatgcct tggatccaac tgaaactgaa caccaccggc
    19321 gcgaacgcgg aagatcttag cgatgcgctg atggaagcgg gtgccgtttc tatcactttt
    19381 caggataccc acgatacgcc tgtgtttgag ccgctgccgg gcgaaacgcg cctgtggggc
    19441 gacaccgatg tgattggtct gttcgacgct gaaaccgata tgaacgacgt ggtggcgatt
    19501 ctggaaaacc atccgctgct cggcgcgggc ttcgcgcata aaatcgaaca actggaagat
    19561 aaagactggg agcgcgaatg gatggataat ttccacccga tgcgctttgg tgaacggctg
    19621 tggatctgcc caagctggcg tgatgtgccg gacgaaaacg ccgtcaacgt gatgttagat
    19681 ccagggctgg cgtttggtac gggtacccat ccaactacct ctctgtgcct gcagtggctc
    19741 gacagcctcg atttaaccgg taaaacagtc atcgactttg gctgcggttc cggcattctg
    19801 gctatcgcgg cgctgaaact gggtgcagca aaagccattg gtattgatat tgatccgcag
    19861 gcgattcagg ccagccgcga caacgcggaa cgtaatggcg tctctgaccg tctggaactc
    19921 tacttaccga aagatcagcc agaagaaatg aaagccgacg tggtggtcgc taacatcctt
    19981 gcaggcccat tacgtgaact ggcaccgtta atcagcgtcc tgccagtttc aggcggtttg
    20041 ctgggccttt ccggtattct ggcaagccag gcagagagcg tttgcgaagc ttatgccgac
    20101 agcttcgctc tggacccggt cgtggaaaaa gaagagtggt gccgcattac cggtcgtaag
    20161 aattaacctt cgcatcgccg tagggtgacg cagagcgaag tgcgagcgat gtcacaaaag
    20221 gcacgttaat ttgccgatta tttacgcaaa tttgcgtgcc aaaattttca ttcataaaga
    20281 aaaattgaga acttactcaa atttcttgga gtgtaaattt tagtcactat tttctaatat
    20341 gatgattttt atgagtaatt atcgcgccac gctcatttta aatgcaattc tttgatccat
    20401 ctcagaggat tggtcaaagt ttggcctttc atctcgtgca aaaaatgcgt aatatacgcc
    20461 gccttgcagt cacagtatgg tcatttctta actcatgcgc atcggacaat atcagctcag
    20521 aaatcgcctg atcgcagcgc ccatggctgg cattacagac agaccttttc ggacgttgtg
    20581 ctacgagatg ggagccggat tgacagtatc cgagatgatg tcttctaacc ctcaggtttg
    20641 ggaaagcgac aaatctcgtt tacggatggt gcacattgat gaacccggta ttcgcaccgt
    20701 gcaaattgct ggtagcgatc cgaaagaaat ggcagatgca gcacgtatta acgtggaaag
    20761 cggtgcccag attattgata tcaatatggg ttgcccggct aaaaaagtga atcgcaagct
    20821 cgcaggttca gccctcttgc agtacccgga tgtcgttaaa tcgatcctta ccgaggtcgt
    20881 caatgcagtg gacgttcctg ttaccctgaa aattcgcact ggctgggcac cggaacaccg
    20941 taactgcgaa gagattgccc aactggctga agactgtggc attcaggctc tgaccattca
    21001 tggccgtaca cgcgcctgtt tgttcaatgg agaagctgag tacgacagta ttcgggcagt
    21061 taagcagaaa gtttccattc cggttatcgc gaatggcgac attactgacc cgcttaaagc
    21121 cagagctgtg ctcgactata caggggcgga tgccctgatg ataggccgcg cagctcaggg
    21181 aagaccctgg atctttcggg aaatccagca ttatctggac actggggagt tgctgccccc
    21241 gctgcctttg gcagaggtta agcgcttgct ttgcgcgcac gttcgggaac tgcatgactt
    21301 ttatggtccg gcaaaagggt accgaattgc acgtaaacac gtttcctggt atctccagga
    21361 acacgctcca aatgaccagt ttcggcgcac attcaacgct attgaggatg ccagcgaaca
    21421 gctggaggcg ttggaggcat acttcgaaaa ttttgcgtaa acagaaataa agagctgaca
    21481 gaactatgtt cgaacaacgc gtaaattctg acgtactgac cgtttctacc gttaactctc
    21541 aggatcaggt aacccaaaaa cccctgcgtg actcggttaa acaggcactg aagaactatt
    21601 ttgctcaact gaatggtcag gatgtgaatg acctctatga gctggtactg gctgaagtag
    21661 aacagcccct gttggacatg gtgatgcaat acacccgtgg taaccagacc cgtgctgcgc
    21721 tgatgatggg catcaaccgt ggtacgctgc gtaaaaaatt gaaaaaatac ggcatgaact
    21781 aattcaggtt agctaaatgc ttgattaaaa aggcgctact cggcatgggg aagcgccttt
    21841 tttataggtg tcacaaaggg agtgaccatg agaacaggat gtgaaccgac ccgatttggt
    21901 aatgaagcaa agaccatcat ccagggtgat gcattaaccg aacttaaaaa gctccccgcc
    21961 gaaagcgtcg atctgatctt tgccgaccca ccgtataaca tcggtaaaaa ttttgatggt
    22021 ctgatcgaag cctggaaaga agatctgttt atcgactggc tgtttgaagt gattgcagag
    22081 tgccaccgcg ttctgaaaaa gcagggcagc atgtacatca tgaacagcac ggaaaacatg
    22141 ccctttatcg atctccagtg ccgcaagctt tttaccatca aaagtcgcat cgtctggtca
    22201 tatgacagtt caggagtgca ggcgaaaaaa cactacggct ccatgtacga acccatcctg
    22261 atgatggtga aagacgcaaa gaactacaca ttcaacggtg atgctgttct ggtagaagcc
    22321 aaaaccggat cgcagcgcgc gttgatcgat tatcgcaaaa atcctccaca gccttacaat
    22381 catcaaaaag taccgggtaa cgtctgggat tttcctcgcg tgcgttattt gatggatgaa
    22441 tatgaaaacc acccgacgca aaaaccggaa gccttactga aacgcattat tctcgcctct
    22501 tccaacccag gcgatatcgt tctcgacccg tttgccggta gctttactac cggtgccgta
    22561 gccgtcgcca acgggcgaaa attcattggt attgagatca acagcgagta catcaaaatg
    22621 gggcttcgac ggctggatgt cgcgtcgcat tactctgcgg aagaactggc gaaagtgaaa
    22681 aaaagaaaga ccggcaacct gtcaaaacga agccggttaa gcgaagttga ccccgatctc
    22741 attgcaaagt aaaggaagtg taagcctggt tttcagatta ttcatttcgt gtatatttcc
    22801 tgccagactt ggttaaacat gcacaggctc aggtaatgat tcgcaagtat tggtggctcg
    22861 tcgttttcgc tgtcttcgtt tttctgtttg atactttact gatgcagtgg attgaactgc
    22921 tggcaacaga aacagacaaa tgccgcaata tgaactcagt taatccacta aaactggtta
    22981 actgtgacga actga
//
LOCUS       DAZFLD010000064        22720 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.064, whole genome shotgun sequence.
ACCESSION   DAZFLD010000064 DAZFLD010000000
VERSION     DAZFLD010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22720
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.064"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..177)
                     /locus_tag="ACHKBL_002101"
     CDS             complement(<1..177)
                     /locus_tag="ACHKBL_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE9 sensor domain-containing protein"
                     /protein_id="HFO5818977.1"
                     /translation="MLSLYEKIKIRLIILFLLAALSFIGLFFIINYQLVSERAVKRAD
                     SRFELIQKNVGYFFK"
     gene            complement(354..1265)
                     /gene="gsiD"
                     /locus_tag="ACHKBL_002102"
     CDS             complement(354..1265)
                     /gene="gsiD"
                     /locus_tag="ACHKBL_002102"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HFO5818978.1"
                     /translation="MRLFNWRRQAVLNAMPLVKPDQVRTPWHEFWRRFRRQHMAMTAA
                     LFVILLIVVAIFARWIAPYDAENYFDYDNLNNGPSLQHWFGVDSLGRDIFSRVLVGAQ
                     ISLAAGVFAVFIGAAIGTLLGLLAGYYEGWWDRLIMRICDVLFAFPGILLAIAVVAVL
                     GSGIANVIIAVAIFSIPAFARLVRGNTLVLKQQTFIESARSIGASDMTILLRHILPGT
                     VSSIVVFFTMRIGASIISAASLSFLGLGAQPPTPEWGAMLNEARADMVIAPHVAVFPA
                     LAIFLTVLAFNLLGDGLRDALDPKIKG"
     gene            complement(1268..2188)
                     /gene="gsiC"
                     /locus_tag="ACHKBL_002103"
     CDS             complement(1268..2188)
                     /gene="gsiC"
                     /locus_tag="ACHKBL_002103"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiC"
                     /protein_id="HFO5818979.1"
                     /translation="MLNYVIKRLLGLIPTLFIVSVLVFLFVHMLPGDPARLIAGPEAD
                     AQVIELVRQQLGLDQPLYHQFWHYISNAVQGDFGLSMVSRRPVGDEIASRFMPTLWLT
                     ITSMVWAVIFGMAAGIIAAVWRNRWPDRLSMTIAVSGISFPAFALGMLLIQVFSVELG
                     WLPTVGADSWQHYILPSLTLGAAVAAVMARFTRASFVDVLSEDYMRTARAKGVSETWV
                     VLKHGLRNAMIPVVTMMGLQFGFLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQA
                     EILLFSLEFILINLVVDVLYAAINPAIRYK"
     gene            complement(2206..3744)
                     /gene="gsiB"
                     /locus_tag="ACHKBL_002104"
     CDS             complement(2206..3744)
                     /gene="gsiB"
                     /locus_tag="ACHKBL_002104"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0034634 - glutathione transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034775 - glutathione transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
                     /protein_id="HFO5818980.1"
                     /translation="MARAVHRSGLVALGIATALMASCAFAAKDVVVAVGSNFTTLDPY
                     DANDTLSQAVAKSFYQGLFGLDKEMKLKNVLAESYTVSDDGLTYTVKLREGIKFQDGT
                     DFNAAAVKANLDRASDPANHLKRYNLYKNIAKTEAIDPTTVKITLKQPFSAFINILAH
                     PATAMISPAALEKYGKEIGFHPVGTGPYELDTWNQTDFVKVKKFAGYWQPGLPKLDSI
                     TWRPVADNNTRAAMLQTGEAQFAFPIPYEQAALLEKNKNIELMASPSIMQRYISMNVT
                     QKPFDNPKVREALNYAINRPALVKVAFAGYATPATGVVPPSIAYAQSYKPWPYDPVKA
                     RELLKEAGYPNGFSTTLWSSHNHSTAQKVLQFTQQQLAQVGIKAQVTAMDAGQRAAEV
                     EGKGQKESGVRMFYTGWSASTGEADWALSPLFASQNWPPTLFNTAFYSNKQVDDFLAQ
                     ALKTNDPAEKTRLYKAAQDIIWQESPWIPLVVEKLVSAHSKNLTGFWIMPDTGFSFED
                     ADLQ"
     gene            complement(3764..5635)
                     /gene="gsiA"
                     /locus_tag="ACHKBL_002105"
     CDS             complement(3764..5635)
                     /gene="gsiA"
                     /locus_tag="ACHKBL_002105"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter ATP-binding protein
                     GsiA"
                     /protein_id="HFO5818981.1"
                     /translation="MPHSDELDAGDVLAVENLNIAFMQDQQKIAAVRNLSFSLQRGET
                     LAIVGESGSGKSVTALALMRLLEQAGGLVQCDQMLLRRRSREVIELSEQSAAQMRHVR
                     GADMAMIFQEPMTSLNPVFTVGEQIAESIRLHQNASREEAMVEAKRMLDQVRIPEAQT
                     ILSRYPHQLSGGMRQRVMIAMALSCRPAVLIADEPTTALDVTIQAQILQLIKVLQKEM
                     SMGVIFITHDMGVVAEIADRVLVMYQGEAVETGSVEQIFHAPQHPYTRALLAAVPQLG
                     AMKGLDYPRRFPLISLEHPAKQEPPIEQKTVVDGEPVLRVRNLVTRFPLRSGLLNRVT
                     REVHAVEKVSFDLWPGETLSLVGESGSGKSTTGRALLRLVESQGGEIIFNGQRIDTLS
                     SGKLQALRRDIQFIFQDPYASLDPRQTIGDSIIEPLRVHGLLPGKEAAARVAWLLERV
                     GLLPEHAWRYPHEFSGGQRQRICIARALALNPKVIIADEAVSALDVSIRGQIINLLLD
                     LQRDFGIAYLFISHDMAVVERISHRVAVMYRGQIVEIGPRRAVFENPQHPYTRKLLAA
                     VPVAEPSRQRPQRVLLSDDLPSNIHLRGEEVVAVSLQCVGPGHYVAQPQSEYAFMRR"
     gene            complement(5622..6587)
                     /gene="iaaA"
                     /locus_tag="ACHKBL_002106"
     CDS             complement(5622..6587)
                     /gene="iaaA"
                     /locus_tag="ACHKBL_002106"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HFO5818982.1"
                     /translation="MGKAVIAIHGGAGAISRAQMSLQQELRYIEALSAIVETGQKMLE
                     AGESALDVVTEAVRLLEECPLFNAGIGAVFTRDETHELDACVMDGNTLKAGAVAGVSH
                     LRNPVLAARLVMEQSPHVMMIGEGAENFAFAHGMERVSPEIFSTPLRYEQLLAAREEG
                     AMVLDHSGAPLDEKQKMGTVGAVALDLDGNLAAATSTGGMTNKLPGRVGDSPLVGAGC
                     YANNASVAVSCTGTGEVFIRALAAYDIAALMDYGGLSLAEACERVVMEKLPALGGSGG
                     LIAIDHEGNVALPFNTEGMYRAWGYAGDTPTTGIYREKGDTVATQ"
     gene            6791..8026
                     /gene="moeA"
                     /locus_tag="ACHKBL_002107"
     CDS             6791..8026
                     /gene="moeA"
                     /locus_tag="ACHKBL_002107"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HFO5818983.1"
                     /translation="MEFTTGLMSLDTALNEMLSRVTPLTAQETLPLVQCFGRILASDV
                     VSPLDVPGFDNSAMDGYAVRLADIASGQPLPVAGKSFAGQPYHGEWPAGTCIRIMTGA
                     PVPEGCEAVVMQEQTEQTDNGVRFTAEVRSGQNIRRRGEDISAGAVVFPAGTRLTTAE
                     LPVIASLGIAEVPVIRKVRVALFSTGDELQLPGQPLGDGQIYDTNRLAVHLMLEQLGC
                     EVINLGIIRDDPHALRAAFIEADSQADVVISSGGVSVGEADYTKTILEELGEIAFWKL
                     AIKPGKPFAFGKLSNSWFCGLPGNPVSATLTFYQLVQPLLAKLSGNTASGLPARQRVR
                     TASRLKKTPGRLDFQRGVLQRNADGELEVTTTGHQGSHIFSSFSLGNCFIVLERDRGN
                     VEVGEWVEVEPFNALFGGL"
     gene            8026..8775
                     /gene="moeB"
                     /locus_tag="ACHKBL_002108"
     CDS             8026..8775
                     /gene="moeB"
                     /locus_tag="ACHKBL_002108"
                     /EC_number="2.7.7.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HFO5818984.1"
                     /translation="MAELSDQEMLRYNRQIILRGFDFDGQEALKDSRVLVVGLGGLGC
                     AASQYLASAGVGNLTLLDFDTVSLSNLQRQTLHSDATVGQPKVESARDALTRINPHIA
                     ITPVNALLDDAELAAMIAKHDLVLDCTDNVAVRNQLNAGCFAAKVPLVSGAAIRMEGQ
                     ITVFTYQDGEPCYHCLSRLFGENALTCVEAGVMAPLIGVIGSLQAMEAIKLLAGYGKP
                     ASGKIVMYDAMTCQFREMKLMRNPGCGVCGQ"
     gene            complement(8851..9513)
                     /gene="fsa"
                     /locus_tag="ACHKBL_002109"
     CDS             complement(8851..9513)
                     /gene="fsa"
                     /locus_tag="ACHKBL_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HFO5818985.1"
                     /translation="MELYLDTSDVVAVKTLSRIFPLAGVTTNPSIIAAGKKPLEVVLP
                     QLHEAMGGQGRLFAQVMATTAEGMVNDARKLRSIIADIVVKVPVTAEGLAAIKMLKAE
                     GIPTLGTAVYGAAQGLLSALAGAEYVAPYVNRIDAQGGSGIQTVTDLHQLLKMHAPQA
                     KVLAASFKTPRQALDCLLAGCESITLPLDVAQQMISYPAVDAAVAKFEQDWLGAFGRT
                     SI"
     gene            9644..10543
                     /gene="ybiY"
                     /locus_tag="ACHKBL_002110"
     CDS             9644..10543
                     /gene="ybiY"
                     /locus_tag="ACHKBL_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HFO5818986.1"
                     /translation="MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDL
                     LYDPRLCLEGCELCAKTAPEVIERALNGLLIHREKLTPEHFTALTDCCPTQALTVCGE
                     VKSVEEIMATVLRDKPFYDRSGGGLTLSGGEPFMQPEMATALLQASHEAGIHTAVETC
                     LHVPWKYIAPSLPYIDLFLADLKHVADAPFKQWTDGNAARVLDNLKKLAAAGKKIIIR
                     VPLIQGFNADETSVKAITDFAADELHVSEIHFLPYHTLGINKYHLLNLPYDAPEKPLD
                     APELLDFAQQYACQKGLTATLRG"
     gene            10549..12981
                     /gene="ybiW"
                     /locus_tag="ACHKBL_002111"
     CDS             10549..12981
                     /gene="ybiW"
                     /locus_tag="ACHKBL_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HFO5818987.1"
                     /translation="MTTLKLDTLSDRIKAHKNALVHIVKPPVCTERAQHYTEMYQQHL
                     DKPIPVRRALALAHHLANRTIWIKHDELIIGNQASEVRAAPIFPEYTVSWIEKEIDDL
                     ADRPGAGFAVSEENKRVLHEVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMT
                     SGDAHLAVNFPLLLEKGLDGLREKVAERRSRINLTVLEDLHGEQFLKAIDIVLVAVSE
                     HIERFAALAREMAATETRESRRDELLTIAENCDLIAHQPPQTFWQALQLCYFIQLILQ
                     IESNGHSVSFGRMDQYLYPYYRRDVELNQTLDREHAIEMLHSCWLKLLEVNKIRSGSH
                     SKASAGSPLYQNVTIGGQNLVDGQPMDAVNPLSYAILESCGRLRSTQPNLSVRYHAGM
                     SNDFLDACVQVIRCGFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKW
                     GYRCTGMSFINFARVMLAALEGGRDATSGKVFLPQAKALSAGNFNNFDEVMDAWDTQI
                     RYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSGLQVGI
                     ANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFDGLTHEQLRQRLINGAPKYGNDDDT
                     VDTLLARAYQTYIDELKQYHNPRYGRGPIGGNYYAGTSSISANVPFGAQTMATPDGRK
                     AHTPLAEGASPASGTDHLGPTAVIGSVGKLPTAAILGGVLLNQKLNPATLENESDKQK
                     LMILLRTFFEVHKGWHIQYNIVSRETLLEAKKHPDQYRDLVVRVAGYSAFFTALSPDA
                     QDDIIARTEHML"
     gene            13127..13942
                     /gene="ybiV"
                     /locus_tag="ACHKBL_002112"
     CDS             13127..13942
                     /gene="ybiV"
                     /locus_tag="ACHKBL_002112"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase YbiV"
                     /protein_id="HFO5818988.1"
                     /translation="MSVKVIVTDMDGTFLNDAKTYNQPRFMAQYQELKKRGIEFVVAS
                     GNQYYQLISFFPELKDEISFVAENGALVYEHGKQLFHGELTRHESRIVIGELLKDKQL
                     NFVACGLQSAYVSENAPEAFVALMAKHYHRLKPVKDYQEIDDVLFKFSLNLPDEQIPL
                     VIDKLHIALDGIMKPVTSGFGFIDLIIPGLHKANGISRLLKRWDLSPQNVVAIGDSGN
                     DAEMLKMARYSFAMGNAAENIKQIARYATDDNNHEGALNVIQAVLDNIAPFNS"
     gene            14094..15353
                     /locus_tag="ACHKBL_002113"
     CDS             14094..15353
                     /locus_tag="ACHKBL_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001516052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1479 domain-containing protein"
                     /protein_id="HFO5818989.1"
                     /translation="MTFSCETLPTDAKAAIREMKAALREQLSDVQQVFDTLSAKIETR
                     VAEIDALKAQGLPVWPEVSYSDIAAGTVSDATRNEIKRRGCAVIKGHFPREQAMAWDR
                     AMLDYLDINHFDDVYKGPGDSFFGSLEASRPEIYPIYWSQAQMQARQSENMAAVQSFL
                     NRLWKSESNGKQWFNPDISVIYPDRIRRRPPGTTSKGLGAHTDSGALERWLLPAYQRV
                     FANVFNGNFDDYDPWDAAHRTDVEEYTVDNTTKCSVFRTFQGWTALSDMLSGQGLLHV
                     VPIPEAMAYVLLRPLLDDVPDDELCGVAPGKVLPISEKWHPLLLKALTSIPAITAGDS
                     VWWHCDVIHSVAPVDNQQGWGNVMYIPAAPMCEKNRAYAHKVKMALEKGASPGDFPRE
                     DYEANWEGRFTLEDLNIHGKRALGMDV"
     gene            complement(15594..17186)
                     /gene="ybiT"
                     /locus_tag="ACHKBL_002114"
     CDS             complement(15594..17186)
                     /gene="ybiT"
                     /locus_tag="ACHKBL_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HFO5818990.1"
                     /translation="MLVSSNVTMQFGSKPLFENISVKFGGGNRYGLIGANGSGKSTFM
                     KILGGDLEPTLGNVSLDPNERIGKLRQDQFAFEEFTVLDTVIIGHKELWEVKQERDRI
                     YALPEMSEEDGYKVADLEVKYGEMDGYSAEARAGELLLGVGIPVEQHYGPMSEVAPGW
                     KLRVLLAQALFADPDILLLDEPTNNLDIDTIRWLEQVLNERDSTMIIISHDRHFLNMV
                     CTHMADLDYGELRVYPGNYDEYMTAATQARERLLADNAKKKAQIAELQSFVSRFSANA
                     SKSRQATSRARQIDKIKLEEVKASSRQNPFIRFEQDKKLFRNALEVEGLTKGFDNGPL
                     FKNLNLLLEVGEKLAVLGTNGVGKSTLLKTLVGDLQPDSGTVKWSENARIGYYAQDHE
                     YEFENDLTVFEWMSQWKQEGDDEQAVRSILGRLLFSQDDIKKPAKVLSGGEKGRMLFG
                     KLMMQKPNILIMDEPTNHLDMESIESLNMALELYQGTLIFVSHDREFVSSLATRILEI
                     TPERVIDFSGNYEDYLRSKGIE"
     gene            17405..18325
                     /gene="ldtB"
                     /locus_tag="ACHKBL_002115"
     CDS             17405..18325
                     /gene="ldtB"
                     /locus_tag="ACHKBL_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFO5818991.1"
                     /translation="MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVIT
                     IPEGNTQPLEYFAAEYQMGLSNMMEANPGVDTFLPKGGTVLNIPQQLILPDTVHEGIV
                     INSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVERKKAGPTWTPTAKMHAE
                     YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDI
                     KFLFEKVPVGTRVQFIDEPVKATTEPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKS
                     VQTVTGQPDVDQVVLDEAIKNRSGMPVRLN"
     gene            complement(18383..19501)
                     /gene="ybiR"
                     /locus_tag="ACHKBL_002116"
     CDS             complement(18383..19501)
                     /gene="ybiR"
                     /locus_tag="ACHKBL_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HFO5818992.1"
                     /translation="MSLPFLRTLQGDRFFQLLILVGIGLSFFVPFAPKSWPAAIDWHT
                     IITLSGLMLLTKGVELSGYFDVLGRKMVRRFATERRLAMFMVLAAALLSTFLTNDVAL
                     FIVVPLTITLKRLCEIPVNRLIIFEALAVNAGSLLTPIGNPQNILIWGRSGLSFAGFI
                     AQMAPLAGAMMLTLLLLCWCCFPGKALQYHTGAQTPEWKPRLVWSCLGLYIVFLMALE
                     LKQELWGLVIVAVGFALLARRVVLSVDWTLLLVFMAMFIDVHLLTQLPALQGVLSNVS
                     HLSEPGLWLTAIGLSQVISNVPSTILLLNYVPPSLLLAWAVNVGGFGLLPGSLANLIA
                     LRMANDRRIWWRFHLYSIPMLLWAALVGYVLLVMIPAW"
     gene            complement(19498..19965)
                     /gene="mntR"
                     /locus_tag="ACHKBL_002117"
     CDS             complement(19498..19965)
                     /gene="mntR"
                     /locus_tag="ACHKBL_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-binding transcriptional regulator
                     MntR"
                     /protein_id="HFO5818993.1"
                     /translation="MSRRAGTPTAKKVTQLVNVEEHVEGFRQVREAHRRELIDDYVEL
                     ISDLIREVGEARQVYMAARLGVSQPTVAKMLKRLATMGLIEMIPWRGVFLTAEGEKLA
                     QESRERHQIVENFLLVLGVSPEIARRDAEGMEHHVSEETLDAFRLFTQKHGAK"
     gene            20151..20279
                     /gene="mntS"
                     /locus_tag="ACHKBL_002118"
     CDS             20151..20279
                     /gene="mntS"
                     /locus_tag="ACHKBL_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganase accumulation protein MntS"
                     /protein_id="HFO5818994.1"
                     /translation="MNEFKRCMRVFSHSPFKVRLMLLSMLCDMVNNKPQQDKPSDK"
     gene            20551..22134
                     /gene="opgE"
                     /locus_tag="ACHKBL_002119"
     CDS             20551..22134
                     /gene="opgE"
                     /locus_tag="ACHKBL_002119"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HFO5818995.1"
                     /translation="MNLTLKESLVTRSRVFSPWTAFYFLQSLLINLGLGYPFSLLYTA
                     AFTAILLLLWRTLPRVQKVLVGVSSLVAACYFPFAQAYGAPNFNTLLALHSTNMEEST
                     EILTIFPWYSYLVGLFIFALGVIAIRRKKESEKARWNTFDSLCLVFSVATFFVAPVQN
                     LAWGGVFKLKDTGYPVFRFAKDVIVNNNEVIEEQERMAKLSGMKDTWTVTAVKPKYQT
                     YVVVIGESARRDALGAFGGHWDNTPFASSVNGLIFADYIAASGSTQKSLGLTLNRVVD
                     GKPQFQDNFVTLANRAGFQTWWFSNQGQIGEYDTAIASIAKRADEVYFLKEGNFEADK
                     NTKDEALLDMTAQVLAQEHSQPQLIVLHLMGSHPQACDRTQGKYETFVQSKETSCYLY
                     TMTQTDDLLRKLYDQLRNSGSSFSLVYFSDHGLAFKERGKDVQYLAHDDKYQQNFQVP
                     FMVISSDDKAHRVIKARRSANDFLGFFSQWTGIKAKEINIKYPFISEKKAGPIYITNF
                     QLQKVDYNHLGTDIFDPKP"
     gene            complement(22183..22698)
                     /gene="ompX"
                     /locus_tag="ACHKBL_002120"
     CDS             complement(22183..22698)
                     /gene="ompX"
                     /locus_tag="ACHKBL_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpX"
                     /protein_id="HFO5818996.1"
                     /translation="MKKIACLSALAAVLAFTAGTSVAATSTVTGGYAQSDAQGQMNKM
                     GGFNLKYRYEEDNSPLGVIGSFTYTEKSRTASSGDYNKNQYYGITAGPAYRINDWASI
                     YGVVGVGYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDV
                     GTWIAGVGYRF"
BASE COUNT         5609 a         6197 c         5616 g         5298 t
ORIGIN      
        1 tttaaagaaa tagccaacgt ttttctgaat aagttcaaag cggctatcgg cacgttttac
       61 cgcgcgttcc gataccagtt gatagttaat gatgaaaaaa agaccaataa atgacagtgc
      121 tgccaataaa aataaaatta tcagccttat ctttatcttt tcgtataaac tcagcatgtt
      181 aatacattta acaaaataac tgtctgaacg ctgtgccgct gcaaaatagt cccttgctgc
      241 ggctttttaa ccaaattacc cgattctaca aaatcagcag aaagttacaa cacttgctta
      301 gggcaataat ttcctgtgag gtgattaccc tttcaagcaa tattcaaacg taactatcct
      361 ttaattttcg gatccagcgc atcgcgtaaa ccatcgccca acaaattgaa cgccagtacg
      421 gtcagaaaaa tagccagggc cggaaaaaca gcgacatgcg gcgcgataac catatccgct
      481 cgtgcctcat tgagcattgc accccactct ggtgtcggcg gctgcgcacc gaggccgaga
      541 aatgacaggc tggcggcaga gataatcgag gcgccaatgc gcatggtgaa aaacaccacg
      601 atagaagaga cggtcccagg caagatatga cgcaacaaaa tggtcatatc gctggcacca
      661 atactgcgtg ctgactcgat aaaggtttgc tgtttcaaca ccagcgtgtt gccgcgcacc
      721 agccgggcaa acgcggggat ggaaaaaatg gcgactgcga taatcacgtt agcaatgccg
      781 cttcccaaca ccgcaacaac agcgatcgcc agtaaaatac ccgggaaggc aaacaacaca
      841 tcgcaaatgc gcatgatcag ccgatcccac cagccttcat aatatccagc cagcaagccc
      901 agcaacgtcc cgatcgccgc accgataaac acggcaaaca cgcccgccgc cagcgatatt
      961 tgcgcaccaa ccaggacacg gctgaaaatg tcgcgcccca gtgaatcgac accaaaccag
     1021 tgctgcaaag aaggtccgtt attcagattg tcataatcaa aataattttc ggcgtcatag
     1081 ggagcgatcc agcgtgcaaa aatggccacc acaatcaata aaataacgaa caatgcggcg
     1141 gtcatcgcca tatgctgacg gcgaaatcgt cgccagaatt catgccacgg tgtacgtacc
     1201 tggtcaggtt tgaccagtgg catggcgttt aacaccgcct gacgtcgcca gttaaatagt
     1261 cgcatcctta cttgtacctg atagccgggt taatggcggc gtaaagcaca tccaccacta
     1321 agttgataag aataaattcc agcgagaaaa gcagaatttc cgcctgaatc accgggtaat
     1381 cgcgcatttc aacggagtca acgagtaagc gtccaagtcc gggccagttg aaaactttct
     1441 ccaccacgat ggaaccaccg agtaaaaatc caaactgtaa gcccatcatg gtcactaccg
     1501 ggatcatcgc gttgcgtagc ccgtgtttga ggacaaccca ggtttcgctc acccctttcg
     1561 ccctcgcggt acgcatataa tcttcgctta aaacgtcgac aaacgacgcg cgggtaaagc
     1621 gcgccatcac ggcggccact gccgcgccga gcgtcaggga gggtaaaatg tagtgctgcc
     1681 aactgtctgc tcccacggta ggcagccagc ccagttcaac agagaatacc tgaattaaaa
     1741 gcatccccag agcaaatgcc ggaaacgaaa tccccgacac cgcaatagtc atactcaatc
     1801 gatccggcca acggttacgc cagacggcgg cgataattcc cgccgccata ccaaatatca
     1861 ccgcccagac catactggtt atggtcagcc acagcgttgg cataaagcgg ctggcaatct
     1921 catcgccaac cggacgacgc gacaccatcg acaggccaaa atccccctgc acagcattgc
     1981 tgatatagtg ccagaactgg tgatacagcg gctgatccaa ccccagctgc tgacgcacca
     2041 gttctataac ctgcgcatca gcttcgggcc cggcaattaa tcgcgccgga tcgccgggca
     2101 gcatgtggac aaataaaaac accagcaccg agacgataaa cagcgtcgga atcaacccca
     2161 gtaagcgttt gataacgtaa ttaagcattc cactccctgc gttgcttatt gcaaatccgc
     2221 atcttcaaag ctgaagccgg tgtctggcat gatccaaaaa ccggtcaggt ttttactgtg
     2281 tgccgacaca agtttttcta ccaccagcgg gatccacggc gactcttgcc agatgatatc
     2341 ctgcgccgcc ttatacaagc gggtcttttc cgccggatca ttagttttca gtgcctgagc
     2401 caggaagtca tccacctgtt tattgctgta aaacgcggta ttaaacagcg ttggtggcca
     2461 gttctgcgag gcaaacagcg gcgatagagc ccagtcggct tcgccggttg aagccgacca
     2521 gccagtgtag aacatccgca cgccgctctc tttttgccct ttaccttcaa cttctgccgc
     2581 ccgctgtccg gcatccatcg cagtcacctg ggctttaatc ccaacctgcg ctaactgctg
     2641 ctgggtaaat tgcagcactt tctgcgcggt gctgtggtta tgtgacgacc acagcgtggt
     2701 actgaaaccg ttgggatatc ccgcctcttt cagtaattcg cgcgctttca ctggatcgta
     2761 aggccacggt ttataacttt gcgcgtaggc gatactcggc ggtaccacac cagtagctgg
     2821 cgttgcatag cccgcgaagg ccactttcac caacgccggg cggttaatgg cgtaattcag
     2881 cgcctcacgg accttcgggt tatcgaacgg cttttgcgtc acgttcatac tgatataacg
     2941 ctgcataatt gacggactgg ccatcaactc gatatttttg tttttctcca gcagtgcggc
     3001 ctgctcgtag ggaatgggga aagcaaactg cgcttcaccg gtttgcagca ttgccgcgcg
     3061 ggtgttgtta tccgccaccg gacgccaggt tatgctgtcc agtttgggca atcctggctg
     3121 ccagtaaccc gcgaattttt tcaccttcac aaaatcggtc tgattccagg tatccagttc
     3181 atacggcccg gttcccaccg gatgaaaacc aatctccttg ccatattttt ccagcgctgc
     3241 cggtgaaatc atcgcggtcg ccggatgggc aagaatatta ataaacgctg agaatggctg
     3301 tttgagggta atctttaccg ttgtcggatc gatcgcttcc gttttggcaa tattcttata
     3361 caggttatag cgtttaagat gattcgccgg atcgctggcc cggtccagat tcgcttttac
     3421 cgccgctgcg ttgaagtcgg ttccatcctg gaatttaatg ccttcccgca gtttcacggt
     3481 gtaagtaagg ccgtcatcgg aaacggtata actctccgcc agcacgtttt tcagtttcat
     3541 ctctttatcc agaccgaaca gcccctggta aaacgatttc gctacggcct gagataacgt
     3601 gtcattcgcg tcatatggat cgagcgtggt gaagttcgat cctaccgcca ccaccacatc
     3661 tttggcagcg aatgcacaag atgccatcaa cgctgtcgca atgcccagcg ccactaaccc
     3721 actacggtgt acagctcttg ccattttatt ctccgcctga atgttatcta cgcatgaatg
     3781 cgtattctga ttgtggttgt gcgacgtaat gccccggccc gacgcattgc aacgagacgg
     3841 ctacaacctc ttcaccacgc agatgaatat tgctgggaag atcgtccgac agcagtacac
     3901 gctgcggtcg ttgtcgggac ggttcagcga ccggaactgc cgccagtaat ttacgcgtat
     3961 aaggatgctg cgggttttcg aagaccgcgc gccgtggacc aatttcaaca atttgtccgc
     4021 gatacatcac cgccacacga tgactaatcc gctctaccac ggccatatcg tgggagataa
     4081 acagatacgc aatgccgaaa tcacgctgga gatcgagcaa caagttgata atctgcccgc
     4141 gaatagaaac atccagcgcc gagacggctt cgtcggcaat gatcactttt ggattcaatg
     4201 ccaacgcgcg agcaatgcag atgcgctggc gctgaccgcc ggaaaactca tgagggtaac
     4261 gccaggcatg ttccggtaac aggcccacgc gctccagcaa ccacgcaacg cgtgcagccg
     4321 cttctttacc tggcaataaa ccgtgtacgc gcagcggttc gataatcgaa tcaccgatgg
     4381 tctgacgtgg gtccagcgaa gcgtaagggt cctgaaaaat aaactgaata tcccggcgca
     4441 aagcctgaag tttaccggat gacaaggtat cgattcgctg accgttaaag ataatttcgc
     4501 cgccctgcga ttcgaccagg cgcagcaacg cccgcccggt agtggattta ccgctgccag
     4561 actcgcccac cagcgatagc gtttcgccag gccagagatc aaaactgact ttctcaacgg
     4621 catgcacttc ccgcgttacg cgattcaaca aaccgctgcg caaagggaaa cgggtgacca
     4681 gattacgcac ccgtaaaaca ggttcgccat ccaccaccgt tttctgctcg atggggggtt
     4741 cctgttttgc tggatgttca agcgatatca atgggaaacg tcggggataa tctaaccctt
     4801 tcatcgcacc aagttgcgga acagcagcta acagcgcacg ggtataagga tgttgcggtg
     4861 catgaaaaat ctgttcgacg ctacccgttt ccaccgcctc gccctgatac atcaccagta
     4921 cccgatcggc aatctctgcc accacgccca tatcgtgagt gataaagata acgcccatcg
     4981 acatctcttt ttgcaatact ttgattaatt gcaggatctg cgcctgaata gtgacatcca
     5041 acgcggtggt tggctcatcg gcaatcagca ccgccgggcg gcatgacagc gccatcgcaa
     5101 tcatcactcg ctggcgcatc ccgccagaga gttgatgcgg ataacgtgaa aggatggttt
     5161 gtgcctccgg aatgcgtacc tgatccagca tccgcttcgc ctcgaccatc gcttcttcac
     5221 gactggcgtt ctgatgcaga cgaattgatt cggcaatctg ttcacccaca gtaaataccg
     5281 ggttcagcga tgtcatcggt tcctgaaata tcatcgccat atccgcaccg cgcacatggc
     5341 gcatttgtgc agcgctctgc tcgctaagtt caatcacttc acgactgcgc cgccgcaaca
     5401 gcatttgatc gcactgcact aaaccgcccg cctgttccaa caagcgcatc aatgccagcg
     5461 cagtcactga cttaccagag ccggattcgc caacaattgc cagcgtctca ccgcgttgca
     5521 ggctaaaaga gagattgcgg accgcagcta ttttctgctg gtcctgcata aaggcaatat
     5581 tcagattttc aaccgccagc acatcaccgg catcaagttc atcactgtgt ggcaacggtg
     5641 tccccttttt cacggtagat accggtggtt ggcgtatcgc ctgcgtagcc ccaggcgcga
     5701 tacattcctt cggtgttaaa cggtagcgcg acgttacctt catggtcgat agcgattaag
     5761 ccaccgctac cgccaagcgc aggaagtttt tccattacta cccgctcgca ggcttccgcg
     5821 agacttaatc cgccgtaatc cattaacgcg gcgatgtcat atgccgccag cgcgcggatg
     5881 aagacttcgc ccgtgccggt acaagaaacc gccacactgg cgttattggc gtagcatccg
     5941 gcacccacta aggggctatc gccaactcgt ccgggtaatt tattggtcat tccgcccgtg
     6001 gacgtagctg ccgccagatt gccgtctaaa tccaacgcca cggcccccac ggtgcccatt
     6061 ttctgttttt catccagtgg cgcaccgcta tggtcgagaa ccattgcccc ttcctcgcgc
     6121 gctgccagta gttgttcata acgcaaaggc gtggagaaaa tctccggcga gacgcgctcc
     6181 atgccatgag caaacgcaaa attttctgcc ccttcgccaa tcatcatcac atgcgggctt
     6241 tgctccatca ccagccgggc ggcaagaacc ggattacgca gatgactaac gcctgccacc
     6301 gcaccggctt tcagggtgtt accatccatc acacaggcgt ccagttcatg ggtttcatca
     6361 cgcgtaaaga cagcgccaat tccggcgtta aacagtggac actcttccag cagacgcacc
     6421 gcttccgtca ccacatccag cgcactttcg cccgcttcca gcattttctg cccggtttca
     6481 acaatggcag acagcgcctc gatgtagcgt aattcctgtt gcagactcat ctgcgcgcgg
     6541 ctaattgcac ctgcgccgcc atgaattgca atgactgctt tgcccatagt attcgtccat
     6601 taagggtggc tcgccacttt tgctataaat atcagaattt ttatgaatta ctcattcaat
     6661 ttttgaatat agaaagatgg atatggcatg taaaggcagg agccaacgct acgcccgagg
     6721 tggcatacag tctgtggcgt ttttctgaca taataggcaa attcgatttt gcctccgcag
     6781 gagtgttttc atggaattta ccaccggatt gatgtcgctc gacaccgcgc ttaatgagat
     6841 gctttctcgc gttaccccac tgaccgccca ggaaacgctg ccactggttc agtgttttgg
     6901 tcgtattctg gcgagcgatg tcgtttcgcc actggatgtc ccggggtttg ataactccgc
     6961 tatggacggc tacgcggtgc gtttagccga tattgcctcc gggcaaccgc tgcccgttgc
     7021 cggtaaatcc tttgccggtc agccgtacca tggtgaatgg cctgcgggta cctgcattcg
     7081 tattatgacc ggtgcgccgg tgccagaagg ctgcgaagcg gtggtgatgc aggagcagac
     7141 tgaacaaacg gacaatggcg tgcgttttac tgctgaagtg cgtagcgggc aaaatattcg
     7201 ccgtcgcggt gaagatatct ctgcaggtgc ggttgttttc ccggcgggga ctcgcctgac
     7261 taccgctgaa ctgccagtga ttgcttcgct agggattgcc gaagttccgg tgattcgtaa
     7321 agtgcgtgta gcgctttttt ctaccggtga tgaactccag ttgcccggtc agccgctggg
     7381 cgacggccaa atctacgata ccaaccgtct cgccgtacac ctgatgttag aacagttggg
     7441 atgcgaggta atcaacttag ggattatccg cgacgatccc catgccctgc gcgccgcatt
     7501 tattgaagcc gacagccagg cggatgtagt gatcagttcc ggcggtgttt cagtgggtga
     7561 agcggattac accaaaacga ttcttgaaga gctgggggag atcgccttct ggaaactggc
     7621 gattaaaccg ggtaaaccgt tcgcgttcgg taaactttct aatagttggt tctgcggcct
     7681 gccgggcaac ccggtttcag cgacgctgac cttctatcaa ctggtacagc ctttgctggc
     7741 aaaactaagc ggcaataccg ccagtggcct gcccgcgcgc cagcgtgtac gcacagcgtc
     7801 ccgcctgaaa aaaacgccag gacgccttga tttccagcgc ggcgtgctgc aacgcaacgc
     7861 cgatggcgaa ctggaagtga cgaccaccgg acatcagggt tcacatatat ttagctcctt
     7921 tagcctcggc aactgcttta tcgtgctgga acgcgatcgc ggcaatgtgg aagtgggcga
     7981 atgggtggaa gtagaaccgt ttaacgcgtt gttcggaggc ctgtaatggc ggaactcagc
     8041 gatcaggaga tgctgcgcta caaccggcaa atcattctgc gcggttttga ttttgacggt
     8101 caggaagcat tgaaagattc ccgcgtgctg gtcgtcggcc ttggtgggct cggctgcgca
     8161 gcttcgcagt atctggcaag cgccggtgtc ggcaacctga cgctgctcga cttcgacacg
     8221 gtttcgctct ccaatctgca acgccagacg ctgcacagtg atgccacggt cgggcaacca
     8281 aaggtggaat ccgcccgtga cgccctgacg cggatcaacc cacatatcgc gattacgcca
     8341 gtcaatgcgc tgcttgatga cgctgaactt gctgcgatga ttgcaaaaca cgatctggtg
     8401 ctcgactgta cggataacgt tgcagtacgt aatcaactga acgcaggctg ttttgccgcg
     8461 aaggtgccgc tggtttccgg cgcggcaatt cgtatggaag ggcaaatcac ggtcttcact
     8521 tatcaggacg gcgaaccgtg ctatcactgc cttagccgtt tgtttggcga gaacgcgtta
     8581 acctgcgtag aagcaggcgt gatggcaccg ttaatcggcg taattggttc gttgcaggca
     8641 atggaagcaa tcaaactgct ggcaggttat ggcaaaccag ccagcggaaa aatcgtgatg
     8701 tacgatgcga tgacctgtca gtttcgcgaa atgaaactga tgcgcaatcc ggggtgtggg
     8761 gtgtgcgggc agtaactgcc acctccaggc cagataagac gcggcactcc gccacgtcat
     8821 cagcaattta tgaggatgca gaacgtccgg ttaaatcgac gttctgccaa acgctcccag
     8881 ccagtcctgt tcaaacttcg ccacagcggc atcaaccgcc ggatagctaa tcatctgttg
     8941 tgccacatcc agcggcagag taattgattc acatcctgcc agtaagcagt ccagcgcctg
     9001 acgcggggtt ttgaaactcg cggctaacac tttcgcctgc ggcgcatgca ttttcaataa
     9061 ctggtgtaag tcggtcacag tctgaatacc gctaccgccc tgagcatcaa tacgattaac
     9121 gtaaggcgca acatattccg cacctgccag cgccgacagt agcccttgtg ctgcgccata
     9181 taccgcggtt cccagcgtcg gaatcccttc cgcttttaac atcttaatag ctgccagccc
     9241 ctcagcggtc accggaacct tcaccacgat atccgcaata atagaacgca atttacgtgc
     9301 gtcattaacc atcccttcag cagtggtagc cattacctgg gcaaacagac gcccctgacc
     9361 gcccatcgcc tcgtgaagtt gcggaagcac aacctccagc ggttttttac ccgctgcgat
     9421 aatgcttggg ttagtggtca cacccgccag cggaaaaata cgtgacagcg tcttcaccgc
     9481 aacaacgtct gaagtatcca gatacagttc cataaccatc ctcaaaatgt actttttgtc
     9541 tgaatgtaga ctatcacggg aaaactctct gaacagttga cgcacatcaa tctaactttc
     9601 attcgaaagt attttaatgt ttgaacgaaa cagagaggca actatgattt tcaatattca
     9661 acgctactcg acccatgatg gccccggtat ccgcacggtc gtatttctta aaggctgttc
     9721 gctgggctgc cgctggtgtc agaacccgga aagccgcgcc cgcacgcagg atctgctgta
     9781 tgacccacga ctctgtctgg aaggctgtga gctgtgcgct aagaccgcac cggaagtgat
     9841 tgaacgcgcg ttgaatggtt tgcttattca tcgggaaaag ttaaccccgg agcattttac
     9901 ggcgctaacc gactgctgcc cgacacaggc gttaaccgtg tgtggtgaag tgaaaagcgt
     9961 tgaggagatc atggcaaccg ttttgcgcga taaaccgttt tacgatcgca gcggcggcgg
    10021 cttaacgctt tctggcggtg aaccgtttat gcagccggaa atggcgacgg cgctgctgca
    10081 agccagccac gaggccggca ttcatactgc ggtagaaacc tgtctgcatg tgccgtggaa
    10141 atatatcgcc ccttctctgc cctatatcga tctgtttctt gccgatttaa aacacgttgc
    10201 cgacgcgccg tttaaacagt ggaccgacgg taacgccgcc agggtgctgg ataacctgaa
    10261 aaaactcgcc gcagcgggca aaaaaatcat tatccgcgtg ccactgattc agggctttaa
    10321 tgccgacgaa acctctgtga aagccattac cgattttgcc gccgacgagc tgcacgttag
    10381 cgaaattcat ttcctgccct accacacgct gggcatcaac aaatatcact tacttaatct
    10441 gccctatgac gccccggaaa aaccgcttga tgcgccagaa ctgctcgact ttgcccagca
    10501 gtatgcctgc cagaaagggt taaccgcgac cttacgagga taacaatcat gacaacacta
    10561 aaactggata cgctcagcga ccgcattaaa gcgcacaaaa atgcgctggt gcatattgtg
    10621 aaaccgccgg tctgtaccga acgagcacaa cactataccg agatgtatca acaacatctc
    10681 gataagccaa tcccggtacg tcgcgcgctg gcactggcgc atcacctggc gaatcgcacc
    10741 atctggatca aacatgatga attgatcatt ggcaaccagg caagcgaagt tcgcgccgcg
    10801 ccgatcttcc cggaatatac cgtctcctgg atcgaaaaag agattgatga tctggcagat
    10861 cgtcccggtg ctggttttgc ggtgagcgaa gagaacaaac gcgttctgca tgaagtgtgc
    10921 ccgtggtggc gcggtcagac cgtacaggat cgctgctacg gcatgtttac cgatgagcaa
    10981 aaaggtctgc tggcgaccgg catcattaaa gcggagggca atatgacctc cggcgatgcg
    11041 cacctggcgg tcaatttccc gctactgctg gaaaaaggtc ttgatggtct gcgtgagaaa
    11101 gtggcggaac gtcgctcgcg catcaatctg acggttctgg aagatctgca cggcgagcaa
    11161 ttcctgaaag cgattgatat cgtgctggtg gcagtcagtg aacacattga acgtttcgct
    11221 gccctggcgc gtgaaatggc cgcaaccgaa acccgcgaaa gccgtcgcga tgaactgctg
    11281 acgatagcag aaaactgcga tcttatcgcc caccagccgc cgcagacttt ctggcaggcg
    11341 ctgcaactgt gctacttcat ccagttgatt ttgcagatcg aatctaacgg tcactcggta
    11401 tcgtttggtc gtatggacca gtatctctac ccatactatc gccgtgacgt tgaactgaac
    11461 cagacactgg atcgcgaaca cgccatcgag atgctgcata gctgctggct gaaactgctg
    11521 gaagtgaaca aaatccgctc cggctcacac tcaaaagcct ctgcgggaag tccgctgtat
    11581 cagaacgtca ccattggcgg gcaaaatctg gttgatggtc aaccgatgga cgcggtaaat
    11641 ccactctctt atgcgatcct cgaatcttgc ggtcgcctgc gttcgactca gcctaacctc
    11701 agcgtgcgtt accatgcggg tatgagtaac gatttcctcg acgcctgtgt tcaggtgatc
    11761 cgctgcggct tcgggatgcc ggcgttcaac aacgacgaaa tcgtgatccc ggaatttatt
    11821 aaactcggta ttgaaccgca ggacgcttac gactacgcag caattggttg tatcgaaacc
    11881 gccgtcggtg gcaaatgggg ctatcgctgt accggcatga gctttatcaa cttcgcccgc
    11941 gtgatgctgg cggcgctgga aggtggtcgt gatgccacca gcggcaaagt gttcctgcca
    12001 caagcaaaag cgttgtcagc gggtaacttc aacaacttcg atgaagtaat ggacgcatgg
    12061 gatacgcaaa tccgttacta cacgcgtaaa tcaatcgaaa tcgaatatgt cgtcgatacc
    12121 atgctggaag agaacgtaca cgatattctc tgctcggcgc tggtcgatga ctgtattgag
    12181 cgagcgaaaa gtatcaagca aggcggcgcg aaatatgact gggtttctgg cctgcaggtc
    12241 ggcattgcta acctcggcaa cagcctggcg gcagtgaaga aactggtatt tgaacagggc
    12301 gcgattggtc agcaacagct ggctgccgca ctggcggatg acttcgacgg cctgactcac
    12361 gagcagctgc gtcagcggct gattaacggt gcgccgaagt acggcaacga cgatgatact
    12421 gtcgatacgc tgctggctcg cgcttatcag acctatatcg acgaactgaa gcagtaccat
    12481 aacccgcgct acggtcgtgg tccgattggc ggcaactatt atgcgggcac atcgtctatc
    12541 tccgctaacg taccgtttgg cgcgcagacg atggcaacgc cggacggacg taaagcacat
    12601 accccgctgg cagaaggcgc tagtccggcc tccggtactg accatctcgg ccctactgcg
    12661 gtcattggct cggtgggtaa actgcctacg gcagcaattc tcggcggcgt gctgctcaac
    12721 cagaaactga atccggcaac gctggagaat gaatctgaca agcagaaact gatgatcctg
    12781 ctgcgtacct tctttgaagt gcataaaggc tggcatattc agtacaacat tgtttcccgc
    12841 gaaacattgc tggaggcgaa gaaacatccg gatcaatacc gcgatctggt ggtacgcgta
    12901 gcgggttatt ccgccttctt caccgcgctc tctccagacg cacaggacga tatcatcgcc
    12961 cgtaccgaac acatgctgta atcccctcag cccggcgtcg tcgtcgccgg gccaaatcat
    13021 tcacatcatc aatttcagcc tcgctttcat tcgaaatata atttgtgctc tgcgtcacat
    13081 tgttattaca ctgttacggt ttcattttcg agccaggagc acacctatga gcgtaaaagt
    13141 tatcgtcaca gacatggacg gtacttttct taacgacgcc aaaacgtaca accaaccacg
    13201 ttttatggcg caatatcagg aactgaaaaa gcgcggcatt gagttcgttg tcgccagcgg
    13261 taatcagtat taccagctta tttcgttctt tcctgagcta aaggatgaga tctcttttgt
    13321 cgcggaaaac ggcgcactgg tttacgaaca tggcaagcag ttgttccacg gcgaactgac
    13381 ccgacatgaa tcgcggattg ttattggcga gttgctaaaa gataagcaac tcaattttgt
    13441 cgcctgcggt ctgcaaagtg catatgtcag cgaaaatgcc cccgaagcat tcgtcgcact
    13501 gatggcaaaa cactaccatc gcctgaaacc tgtaaaagat tatcaggaga ttgacgacgt
    13561 actgttcaag ttttcgctca acctgccgga tgaacaaatc ccgttagtga tcgacaaact
    13621 gcacatagcg ctggatggca ttatgaaacc cgtcaccagt ggttttggct ttatcgactt
    13681 gattattccc ggtctacata aagcaaacgg tatttcgcgg ttactgaaac gctgggatct
    13741 gtcaccgcaa aatgtggtag cgattggcga cagcggtaac gatgcggaga tgctgaaaat
    13801 ggcgcgttat tcctttgcga tgggcaatgc tgcggaaaac attaaacaaa tcgcccgtta
    13861 cgctaccgat gataataatc atgaaggcgc gctgaatgtg attcaggcgg tgctggataa
    13921 tatcgctcct ttcaatagtt gatcttcccc ctggccgaag tgaccacttc ggcctctctc
    13981 ctttttgctc gctttgtgca ctgactcaat atttttatct tgcgttaact tatttttaac
    14041 ttaatgtatc aatcgcaatc attttacttt cgaatgaaag aagcgaggtt gatatgactt
    14101 tttcctgtga gacgctgccc acagatgcga aagcagctat ccgagagatg aaagctgcgc
    14161 tgcgggaaca gctcagtgat gtgcaacagg ttttcgatac actctctgca aaaatcgaaa
    14221 cgcgtgttgc tgaaatcgat gccctaaaag cgcaagggct gccggtttgg ccggaagttt
    14281 cttatagtga tattgccgca ggtacagtaa gcgatgccac tcgcaatgaa attaaacgtc
    14341 gcggttgtgc agtcataaaa ggccatttcc ctcgcgagca ggcgatggcg tgggatcgtg
    14401 ccatgctcga ttatcttgat atcaatcatt ttgatgatgt ttataaaggc cctggcgaca
    14461 gttttttcgg ctcgctggag gcttctcgtc ccgaaatcta tcccatttac tggtcgcaag
    14521 cacagatgca ggcgcggcag agcgaaaaca tggctgctgt gcaatctttt ctaaaccgac
    14581 tgtggaagtc tgaaagcaac ggcaaacagt ggtttaatcc tgatatcagt gtgatttatc
    14641 cagacaggat ccgccgccgc cctccaggta ctacatctaa aggcctgggc gcgcataccg
    14701 attctggtgc gctggaacgg tggttattac ccgcttatca gcgcgttttt gccaatgtgt
    14761 ttaatggtaa tttcgatgat tacgatccct gggatgctgc acaccggacc gatgtcgaag
    14821 agtacacggt agataacact acaaaatgtt cggtgttccg cacgtttcag ggctggacgg
    14881 cgctctctga tatgttatcg ggtcagggat tgttacatgt ggtgcctatc ccggaagcga
    14941 tggcgtatgt tttattgcgc ccgctgctgg atgacgtacc ggacgatgaa ctgtgcgggg
    15001 ttgcacctgg caaagttctg cccatttcag agaaatggca tccactactg cttaaagcgc
    15061 tgacttccat tcccgcgata acagcagggg attccgtctg gtggcactgc gatgtgattc
    15121 actccgtcgc gccagtagac aatcaacagg gttggggcaa cgtgatgtat atccctgctg
    15181 cgccaatgtg cgagaaaaat cgggcttatg cacacaaagt caaaatggcg ctggaaaaag
    15241 gcgcatcacc tggcgacttc ccgcgggaag attatgaagc aaactgggaa ggccgcttta
    15301 cgctggagga tctgaacatt cacggtaagc gcgcactggg catggatgtt tgagagttaa
    15361 tggaagtgaa ttgatgtggg attaagaaaa tcgattcaac gttactcaga ttgctgacaa
    15421 atgcgctttg ttaatgcctg atgcggcgtt aacgccttat ccggcctaca aagtcctgca
    15481 gattcaatat attgcaggta ctgcgtaggc ctgataagca tagcgcatca ggcaatcttg
    15541 cgtttgtcat cagccgccgt caccccgaca gtatcgggct gacggataat aacttactcg
    15601 atccctttac tacgcagata atcttcgtaa ttaccgctaa agtcgatcac gcgttccggg
    15661 gtgatttcca gaattcgggt cgccagggag cttacaaact cacggtcgtg agacacaaag
    15721 atcagcgtgc cctgatacag ttccagcgcc atgttcagcg actcaatgga ttccatatcc
    15781 aggtggttgg tcggttcgtc catgatcaga atgttcggct tctgcatcat taacttacca
    15841 aacagcatcc gccctttctc cccaccggaa agcactttcg ctggcttttt gatgtcgtcc
    15901 tggctgaaca gcaaacgacc gagaatgctg cgtaccgcct gctcgtcatc gccttcctgc
    15961 ttccactgac tcatccattc gaacacggta agatcatttt cgaactcata ttcgtggtcc
    16021 tgggcgtagt aaccaatgcg cgcgttttca gaccatttta cggtgccgct gtccggttgc
    16081 agatcgccca ccagcgtttt caacagtgtt gatttaccga cgccgttggt acccagtacc
    16141 gccagttttt caccgacttc cagcagcagg ttgagatttt taaacagcgg accgttatca
    16201 aaccctttgg tcagaccttc cacttccagc gcgttacgga acagtttctt atcctgttca
    16261 aaacggatga acgggttctg acggctggag gctttcacct cttccagttt gattttatca
    16321 atctggcgcg cgcgcgaagt tgcctggcga gatttcgagg cgttggcact aaagcggcta
    16381 acgaaagatt gcagctcagc aatctgcgct ttcttcttgg cgttatcagc cagcagacgc
    16441 tcacgcgcct gggtcgccgc cgtcatgtac tcatcgtagt tacctggata aacgcgcagc
    16501 tcgccgtaat ccagatccgc catgtgggta caaaccatgt taaggaagtg acggtcgtgc
    16561 gagatgatga tcatggtgct gtcacgctcg ttcagcacct gttccagcca acgaatggtg
    16621 tcgatgtcga ggttgttggt cggttcgtcg agcaggagaa tgtccggatc agcaaacagc
    16681 gcctgcgcca gcagcacacg cagcttccag ccaggagcaa cttcactcat cgggccgtag
    16741 tgctgttcca ctggaattcc cacgccaagc aacagttcac cggcgcgagc ttccgcagag
    16801 taaccgtcca tttcgccgta tttcacttcc agatcggcca ctttatagcc gtcttcttca
    16861 ctcatttccg gcaaagcata gatgcggtca cgctcctgct tcacttccca caactcttta
    16921 tgcccaatga tcaccgtatc cagcacagtg aactcttcaa aggcaaactg atcctgacgc
    16981 agcttaccaa tgcgctcgtt gggatcgagg gaaacgttac ccaacgtcgg ctctaagtcg
    17041 ccgccgagga tcttcataaa ggtggattta ccactaccgt tcgcgccaat caggccgtaa
    17101 cggttgccgc cgccaaattt gacggaaatg ttttcaaaca acggcttact gccgaactgc
    17161 atggtgacgt tactggaaac taacacaggc gtatcctgaa aagagatatg acaaaccgcg
    17221 cattatgcca taaatctgaa agaagatcct gctcctcgct taaggtctac actcaagcca
    17281 aaggcaaaaa atgtgatttc gtacacatct gatttcactg tgagctggaa tgaacttata
    17341 atgcgcttcc aatactctaa tattctcaac ccaatggcct gccaggcaca aaatctcgct
    17401 taacatgaat atgaaattga aaacattatt cgcagcggcc ttcgctgttg tcggcttttg
    17461 cagtaccgcc tctgcggtaa cttatcctct gccaaccgac gggagtcgcc tggttggtca
    17521 gaatcaggtg atcaccattc ctgaaggtaa cactcagccg ctggagtatt ttgccgccga
    17581 gtaccagatg ggactttcca atatgatgga agcgaacccg ggtgtggata ccttcctgcc
    17641 gaaaggcggt actgtactga acattccgca gcagctgatc ctgccggata ccgttcatga
    17701 aggcatcgtc attaacagtg cagagatgcg tctgtattac tatccgaaag gaaccaacac
    17761 cgttatcgtg ctgccgatcg gcattggtca gttaggcaaa gacacgccta tcaactggac
    17821 caccaaagtt gagcgtaaaa aagcaggccc gacctggacg ccgaccgcca aaatgcacgc
    17881 agagtaccgc gctgcgggcg aaccgcttcc ggctgtcgtt ccggcaggtc cggataaccc
    17941 gatgggtctg tatgcactct acatcggtcg cctgtacgct atccatggca ccaacgccaa
    18001 cttcggtatc ggcctgcgtg taagtcatgg ctgtgtgcgt ctgcgtaacg aagacattaa
    18061 attcctgttc gagaaagtgc ctgtcggtac tcgcgtgcag tttattgatg agccggtgaa
    18121 agcgactacc gaaccagacg gcagccgtta cattgaagtc cataacccgc tgtctaccac
    18181 cgaagcccag tttgaaggtc aggaaattgt gccaattacc ctgacgaaga gcgtgcagac
    18241 agtgaccggt cagccagatg ttgaccaggt tgttcttgat gaagcgatca aaaatcgttc
    18301 cgggatgccg gttcgtctga attaatcgtc aaaacgtaaa acaaaggcgg actcataatc
    18361 cgcctttttt atttgccaga ccctaccagg ccgggatcat aaccaacaaa acatatccca
    18421 ccagcgccgc ccacaacagc atcggtatcg aataaagatg aaaacgccac cagatacggc
    18481 gatcgttcgc catccgtaac gcaatcaaat ttgccagcga tccgggtaat aaaccaaagc
    18541 cccctacgtt taccgcccat gccagcagca acgacggcgg cacatagttc agcaacaata
    18601 tggtactcgg cacgttactg atcacctgcg ataaaccgat tgccgttaac cataatccgg
    18661 gttcggataa atgactcacg ttacttaaca ccccctgcaa agctggcagt tgggttagta
    18721 aatggacgtc gataaacatc gccataaaca ccagcagcag cgtccagtcc acactgagca
    18781 ccacgcgacg tgccagcagc gcaaagccca ccgccacaat caccagtccc cacagctctt
    18841 gttttaactc cagcgccatc agaaagacga tatacagccc cagacaactc cacaccagcc
    18901 gcggtttcca ctccggcgtt tgcgcccccg tatggtattg cagcgccttt ccagggaaac
    18961 aacaccagca caacagcagg agtgtcagca tcattgcgcc agccagcggt gccatttggg
    19021 caataaatcc ggcaaacgaa agaccagaac gtccccagat aagaatattt tgcgggttgc
    19081 caattggcgt cagtagcgaa ccagcgttga ctgccagcgc ctcaaaaata atcagccgat
    19141 taaccgggat ctcacacagt ctttttagcg tgatagtcag cggaacaaca ataaacagcg
    19201 cgacatcgtt ggtcagaaag gtagaaagca gcgccgccgc cagcaccata aacatcgcca
    19261 gccgacgctc cgtagcaaag cggcgcacca ttttgcgccc cagcacatca aaataaccgc
    19321 ttaactccac acctttggtc agcagcatca ggccgcttaa ggtgatgatg gtgtgccagt
    19381 cgatagcagc aggccaggat ttcggtgcaa agggaacgaa aaaacttaat ccgatgccaa
    19441 caagaattaa taactgaaaa aaacgatcgc cttgcagcgt gcgtaaaaaa ggcaggctca
    19501 tttggcaccg tgtttctggg taaacaaacg aaaggcatcc agcgtctctt cactaacatg
    19561 gtgctccatg ccttccgcgt cgcgacgggc gatttccgga ctgacgccca acaccaacaa
    19621 gaaattttcg actatctgat gtcgctcgcg gctttcctgc gccagtttct ctccttctgc
    19681 cgttaaaaac acgccacgcc aggggatcat ttcaatcagc cccatggttg ccagtcgctt
    19741 aagcatttta gccaccgtcg gttgcgaaac tcccaaacga gcagccatgt atacctgacg
    19801 agcttccccc acttccctga tcaagtcaga aatcagctca acgtaatcat caatcagctc
    19861 gcgccgatgc gcctctctga cctggcggaa cccttcaacg tgctcttcca cgttcactaa
    19921 ctgcgtcact ttttttgctg ttggcgtacc tgcgcgacga ctcattgtgc ttcctcatta
    19981 tggtgacgca tacatagcgt tctcatttcg tgcgcacatt gtaaaccaga gttgcgaagg
    20041 tacaaaaaat taacgtttta gcaatagcta tataatatag cctgtgctat atctgtatgt
    20101 aatgcaatca tccctcaagg atcgacggga ttagcaagtc aggaggtctt atgaatgagt
    20161 tcaagaggtg tatgcgcgtg tttagtcatt ctccctttaa agtacggtta atgctgctct
    20221 ctatgttgtg cgatatggtc aacaacaaac cgcagcaaga taaaccttca gataaatagc
    20281 ggcgtcgcgg tacgccgctt cactcctgct tttatgcagg cataacgcgt tttggtctga
    20341 aaaaccccac tttttgtcgg atttgcaatc cccttcgcaa aagatttgtt cgtcagtagt
    20401 tgacctgaac ggcggctcgc tctatcttct tgcagccctg cgtatattgc ggctcgcgga
    20461 tgcggacccc tttcccctct ccacgcactc ttgcaggtat tgacccttga cgccaaggta
    20521 agcacatggc gtttgttacg atagtggcat atgaatttaa ccctcaaaga atcgcttgtt
    20581 acccgtagcc gggtatttag cccgtggact gcgttctact ttttacagtc gctattaatt
    20641 aacctcggct taggttatcc cttcagtttg ctctacaccg ctgcgtttac ggctattttg
    20701 cttttgctat ggcgaacatt gcctcgcgta caaaaagttc tggtcggtgt cagttcgctg
    20761 gtggcggctt gttatttccc ttttgctcag gcctacggcg cacctaattt caatacattg
    20821 ctggcattgc actccaccaa tatggaagag tcgaccgaaa tcctgacgat ttttccgtgg
    20881 tacagctacc tggtcggctt atttattttt gcgctcggcg taatagcaat caggcggaaa
    20941 aaagagagtg aaaaagcgcg ctggaatacc ttcgacagcc tgtgtctggt attcagtgtg
    21001 gcgacatttt ttgttgctcc cgtgcaaaac ctggcctggg gtggcgtatt taaactgaaa
    21061 gatactggct atccggtatt tcgttttgct aaggatgtca tcgtcaataa taacgaggtg
    21121 attgaagagc aagaacggat ggcaaaactg tccggaatga aagatacctg gacggtcact
    21181 gccgttaagc cgaagtatca gacctatgtg gtggtgatcg gagaaagcgc gcgtcgcgat
    21241 gccctcggtg cctttggcgg tcactgggac aataccccgt ttgccagcag cgttaacggt
    21301 ttgatatttg ctgactacat tgccgccagt ggctccacgc agaaatcgct tggcttaacg
    21361 ctcaatcgcg ttgtcgatgg caaaccacag tttcaggata actttgtcac cctggcaaat
    21421 cgcgcgggct tccagacctg gtggttttcc aaccagggcc aaatcggcga atacgatacc
    21481 gccatcgcca gcatcgccaa acgtgcagat gaagtgtact tcctgaaaga aggtaatttt
    21541 gaagcagata aaaacacgaa agacgaagcg ttactggata tgaccgctca agtgctggcg
    21601 caagagcact cgcaaccgca gctgattgtt ttacatctga tgggctcaca tccgcaggcc
    21661 tgcgaccgga cacaaggcaa atacgaaacc tttgtgcaat cgaaagaaac gtcgtgctat
    21721 ctctatacca tgacgcaaac agacgattta ctgcgtaagc tgtacgatca gttacgcaac
    21781 agcggcagta gcttctcgct ggtttacttt tctgaccacg gtctggcctt taaagagcgt
    21841 ggcaaagacg tgcaatacct tgcccatgat gataagtatc agcaaaattt ccaggtgcct
    21901 tttatggtca tttcgagcga cgataaagcg catcgggtga ttaaagcccg ccgctctgcc
    21961 aatgacttct taggcttttt ctcccagtgg acgggaatta aagcgaagga aataaatatc
    22021 aaatacccgt ttatatctga gaagaaagcc gggccgatat acatcaccaa cttccagtta
    22081 cagaaggtag attacaacca tctcggaacc gatattttcg acccgaaacc ttaaaaacaa
    22141 aaatccgccc cgagaggcgg attttttata tcaccgaagt gattagaagc ggtaaccaac
    22201 accggcaatc caggtgccta cgtcaacgct acgaatacgg ctctgctcgt aagagaagtc
    22261 cagagcaacg ttttccatcg ggttgaactg cagaccagca ccgtaggaga aaccgtagtc
    22321 gctggtgtcg tgtttgtagg tcgggtattc agtggtctgg aatttaccat aacccacacc
    22381 cactacaccg tagatgcttg cccagtcgtt aatgcggtaa gccggaccag cagtgatgcc
    22441 gtagtactgg tttttgttgt agtcaccaga gcttgcagta cggcttttct cggtgtaagt
    22501 gaaagaaccg atcacaccca gcgggctgtt gtcttcttca tagcggtatt tcaggttgaa
    22561 accgcccatt ttgttcattt ggccctgagc gtcgctctgt gcgtaaccgc cagttacggt
    22621 agaagtcgca gctacggaag tacctgcggt gaaagccaga actgcggcca gtgctgaaag
    22681 acatgcaatt tttttcataa ccacctcaaa tgtgattcaa
//
LOCUS       DAZFLD010000065        22379 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.065, whole genome shotgun sequence.
ACCESSION   DAZFLD010000065 DAZFLD010000000
VERSION     DAZFLD010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22379
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.065"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            46..2397
                     /gene="polB"
                     /locus_tag="ACHKBL_002121"
     CDS             46..2397
                     /gene="polB"
                     /locus_tag="ACHKBL_002121"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HFO5818997.1"
                     /translation="MAQAGFILTRHWRDTPQGTEVSFWLATDNGPLQVTLAPQESVAF
                     IPADQVPRAQHILQGEQGFRLTPLALKDFHRQPVYGLYCRAHRQLMNYEKRLREGGVT
                     VYEADVRPPERYLMERFITSPVWVEGDIRNGAIVNARLKPHPDYRPPLKWVSIDIETT
                     RHGELYCIGLEGCGQRIVYMLGPENGDASALDFELEYVASRPQLLEKLNAWFATHDPD
                     VIIGWNVVQFDLRMLQKHAERYRIPLRLGRDNSELEWREHGFKNGVFFAQVKGRLIID
                     GIEALKSAFWNFSSFSLETVAQELLGEGKSIDNPWDRMDEIDRRFAEDKPALATYNLK
                     DCELVTQIFHKTEIMPFLLERATVNGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLG
                     EVPPHASPGGYVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHS
                     TEGFLDAWFSREKHCLPEIVTNIWHGRDEAKRQGNKPLSQALKIIMNAFYGVLGTTAC
                     RFFDPRLASSITMRGHQIMRQTKALIEAQGYDVIYGDTDSTFVWLKGAHSEEEAAKIG
                     RALVQHVNAWWAETLQKQRLTSALELEYETHFCRFLMPTIRGADTGSKKRYAGLIQEG
                     DKQRMVFKGLETVRTDWTPLAQQFQQELYLRIFRNEPYQEYVRETIDKLMAGELDARL
                     VYRKRLRRPLSEYQRNVPPHVRAARLADEENQKRGRPLQYQNRGSIKYVWTTNGPEPL
                     DYQRSPLDYEHYLTRQLQPVAEGILPFIEDNFATLMTGQLGLF"
     gene            2561..5467
                     /gene="rapA"
                     /locus_tag="ACHKBL_002122"
     CDS             2561..5467
                     /gene="rapA"
                     /locus_tag="ACHKBL_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HFO5818998.1"
                     /translation="MPFTLGQRWISDTESELGLGTVVAVDARTVTLLFPSTGENRLYA
                     RSDSPVTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFL
                     DSKLVFSKPQDRLFAGQIDRMDRFALRYRARKYSSEQFRMPYSGLRGQRTSLIPHQLN
                     IAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPETLQHQWLVE
                     MLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDL
                     LVVDEAHHLVWSEDAPSREYQAIEQLAEHVPGVLLLTATPEQLGMESHFARLRLLDPN
                     RFHDFAQFVEEQKNYRPVADAVAMLLAGNKLSNDELNMLGEMIGEQDIEPLLQAANSD
                     SEDAQSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKV
                     SGIMGARKSAEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLV
                     ICAKAATALQLEQVLREREGIRAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIG
                     SEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIHVPYLEKTAQSVLVRW
                     YHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPDQTEGFDDLIKNCREQHEALKAQLE
                     QGRDRLLEIHSNGGEKAQALAESIEEQDDDTNLIAFAMNLFDIIGINQDDRGDNMIVL
                     TPSDHMLVPDFPGLSEDGITITFDREVALAREDAQFITWEHPLIRNGLDLILSGDTGS
                     STISLLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNGNNLAAQV
                     EFETFNRQLNAVNRHTGSKLVNAVQQDVHAILQLGEAQIEKSARALIDAARNEADEKL
                     SAELSRLEALRAVNPNIRDDELTAIESNRQQVMESLDQAGWRLDALRLIVVTHQ"
     gene            5479..6138
                     /gene="rluA"
                     /locus_tag="ACHKBL_002123"
     CDS             5479..6138
                     /gene="rluA"
                     /locus_tag="ACHKBL_002123"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HFO5818999.1"
                     /translation="MGMENYNPPQEPWLVVLYQDDHIMVVNKPSGLLSVPGRLEEHKD
                     SVMTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFREREPKKQYVARVWG
                     HPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYAADNTARVVLKPITGR
                     SHQLRVHMLALGHPILGDRFYASPEARAMAPRLLLHAEMLTITHPAYGNSMTFKAPAD
                     F"
     gene            complement(6256..7071)
                     /gene="djlA"
                     /locus_tag="ACHKBL_002124"
     CDS             complement(6256..7071)
                     /gene="djlA"
                     /locus_tag="ACHKBL_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HFO5819000.1"
                     /translation="MQYWGKIIGVAVALLMGGGFWGVVLGLLIGHMFDKARSRKMAWF
                     ANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIASQLMDRMNLHGASRTAAQNAFR
                     VGKSDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEE
                     LGISRAQFDQFLRMMQGGAQFGGGYQQQSGGGNWQQAQRGPTLEDACNVLGVKPTDDA
                     TTIKRAYRKLMSEHHPDKLVAKGLPPEMMEMAKQKAQEIQQAYELIKLQKGFK"
     gene            7326..9680
                     /gene="lptD"
                     /locus_tag="ACHKBL_002125"
     CDS             7326..9680
                     /gene="lptD"
                     /locus_tag="ACHKBL_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HFO5819001.1"
                     /translation="MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQ
                     GDTNDLPVTINADHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEAPGQPEPVR
                     TVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGEN
                     RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVPIFYSPYLQL
                     PVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEF
                     RYLSQAGAGLMELDYLPSDKVYEDEHPNDDSSRRWLFYWNHSGVMDQVWRFNVDYTKV
                     SDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEP
                     QLDVNYYQNDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTE
                     AKLLATHYQQTNLDWYNSRNTTKLDESVNRVMPQFKVDGKMVFERDMEMLAPGYTQTL
                     EPRAQYLYVPYRDQSDIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTTGVTSRI
                     YDDAAVERFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISERWGLRG
                     GIQYDTRLDNVATSNSSIEYRRDEDRLVQLNYRYASPEYIQATLPKYYSTAEQYKNGI
                     SQVGAVASWPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVGYERKLNGWD
                     NDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL"
     gene            9733..11019
                     /gene="surA"
                     /locus_tag="ACHKBL_002126"
     CDS             9733..11019
                     /gene="surA"
                     /locus_tag="ACHKBL_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0042277 - peptide binding [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA];
                     GO_process: GO:0050821 - protein stabilization [Evidence
                     IEA];
                     GO_process: GO:0060274 - maintenance of stationary phase
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase SurA"
                     /protein_id="HFO5819002.1"
                     /translation="MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDG
                     LMQSVKLNAAQARQQLPDDATLRHQIMERLIMDQIILQMGQKMGVKISDEQLDQAIAN
                     IAKQNNMTLDQMRSRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESL
                     AQQVGNQNDASTELNLSHILIPLPENPTSDQVNEAESQARAIVDQARNGADFGKLAIA
                     HSADQQALNGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGE
                     SKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGS
                     ANQGGDLGWATPDIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAA
                     QKDRAYRMLMNRKFSEEAASWMQEQRASAYVKILSN"
     gene            11019..12008
                     /gene="pdxA"
                     /locus_tag="ACHKBL_002127"
     CDS             11019..12008
                     /gene="pdxA"
                     /locus_tag="ACHKBL_002127"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HFO5819003.1"
                     /translation="MVKTQRVVITPGEPAGIGPDLVVQLAQREWPVELVVCADATLLT
                     DRAAMLGLPLTLRPYSPNSPAQPQTAGTLTLLPVALRESVTAGQLAVENGHYVVETLA
                     RACDGCLNGEFAALITGPVHKGVINDAGIPFTGHTEFFEERSQAKKVVMMLATEELRV
                     ALATTHLPLRDIADAITPALLHEVIAILHHDLRTKFGIAEPRILVCGLNPHAGEGGHM
                     GTEEIDTIIPVLDKLRAQGMKLNGPLPADTLFQPKYLDNADAVLAMYHDQGLPVLKYQ
                     GFGRGVNITLGLPFIRTSVDHGTALELAGRGKADVGSFITALNLAIKMIVNTQ"
     gene            12005..12826
                     /gene="rsmA"
                     /locus_tag="ACHKBL_002128"
     CDS             12005..12826
                     /gene="rsmA"
                     /locus_tag="ACHKBL_002128"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HFO5819004.1"
                     /translation="MNNRVHQGHLARKRFGQNFLNDQFVIDSIVSAINPQKGQAMVEI
                     GPGLAALTEPVGERLDKLTVIELDRDLAARLQTHPFLGPKLTIYQQDAMTFNFGELAE
                     KMGQPLRVFGNLPYNISTPLMFHLFSYTDAIADMHFMLQKEVVNRLVAGPNSKAYGRL
                     SVMAQYYCNVIPVLEVPPSAFTPPPKVDSAVVRLVPHATMPHPVKDVRVLSRITTEAF
                     NQRRKTIRNSLGNLFSVEVLTGMGIDPAMRAENISVAQYCQMANYLAENAPLQES"
     gene            12829..13206
                     /gene="apaG"
                     /locus_tag="ACHKBL_002129"
     CDS             12829..13206
                     /gene="apaG"
                     /locus_tag="ACHKBL_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HFO5819005.1"
                     /translation="MINSPRVCIQVQSVYIEAQSSPDNERYVFAYTVTIRNLGRAPVQ
                     LLGRYWLITNGNGRETEVQGEGVVGVQPLIAPGEEYQYTSGAIIETPLGTMQGHYEMI
                     DENGVPFSIDIPVFRLAVPTLIH"
     gene            13213..14055
                     /gene="apaH"
                     /locus_tag="ACHKBL_002130"
     CDS             13213..14055
                     /gene="apaH"
                     /locus_tag="ACHKBL_002130"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008803 -
                     bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     ApaH"
                     /protein_id="HFO5819006.1"
                     /translation="MATYLIGDVHGCYDELIALLHKVEFTPGKDTLWLTGDLVARGPG
                     SLDVLRYVKSLGDSVRLVLGNHDLHLLAVFAGISRNKPKDRLTPLLEAPDADELLNWL
                     RRQPLLQIDEEKKLVMAHAGITPQWDLQTAKECARDVEAVLSSDSYPFFLDAMYGDMP
                     NNWSPELRGLGRLRFITNAFTRMRFCFPNGQLDMYSKESPEEAPAPLKPWFAIPGPVA
                     EEYSIAFGHWASLEGKGTPEGIYALDTGCCWGGTLTCLRWEDKHYFVQPSNRHKDLGE
                     AAAS"
     gene            complement(14133..14612)
                     /gene="folA"
                     /locus_tag="ACHKBL_002131"
     CDS             complement(14133..14612)
                     /gene="folA"
                     /locus_tag="ACHKBL_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HFO5819007.1"
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGR
                     HTWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQ
                     FLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     gene            complement(14804..16666)
                     /gene="kefC"
                     /locus_tag="ACHKBL_002132"
     CDS             complement(14804..16666)
                     /gene="kefC"
                     /locus_tag="ACHKBL_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015503 - glutathione-regulated potassium
                     exporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
                     /protein_id="HFO5819008.1"
                     /translation="MDSHTLIQALIYLGSAALIVPIAVRLGLGAVLGYLIAGCIIGPW
                     GLRLVTDAESILHFAEIGVVLMLFIIGLELDPQRLWKLRAAVFGGGALQMVICGGLLG
                     LFCMLLGLRWQVAELIGMTLALSSTAIAMQAMNERNLMVTQMGRSAFAVLLFQDIAAI
                     PLVAMIPLLAASSASTTMGAFALSALKVAGALVLVVLLGRYVTRPALRFVARSGLREV
                     FSAVALFLVFGFGLLLEEVGLSMAMGAFLAGVLLASSEYRHALESDIEPFKGLLLGLF
                     FIGVGMSIDFGTLLENPLRIVILLLGFLIIKIAMLWLIARPLQVPNKQRRWFAVLLGQ
                     GSEFAFVVFGAAQMANVLEPEWAKSLTLAVALSMAATPILLVILNRLEQSSSEEAREA
                     DEIDEEQPRVIIAGFGRFGQITGRLLLSSGVKMVVLDHDPDHIETLRKFGMKVFYGDA
                     TRMDLLESAGAAKAEVLINAIDDPQTNLQLTEMVKEHFPHLQIIARARDVDHYIRLRQ
                     AGVEKPERETFEGALKTGRLALESLGLGPYEARERADVFRRFNIQMVEEMAMVENDTK
                     ARAAVYKRTSAMLSEIITEDREHLSLIQRHGWQGTEEGKHTGNMADEPETKPSS"
     gene            complement(16659..17189)
                     /gene="kefF"
                     /locus_tag="ACHKBL_002133"
     CDS             complement(16659..17189)
                     /gene="kefF"
                     /locus_tag="ACHKBL_002133"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     oxidoreductase KefF"
                     /protein_id="HFO5819009.1"
                     /translation="MILIIYAHPYPHHSHANKRMLEQARTLEGVEIRSLYQLYPDFNI
                     DIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDKVFSHGWAYGHGGTALHGKHLLW
                     AVTTGGGESHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQA
                     RHYKQRLLEWQEAHHG"
     gene            complement(17297..18628)
                     /locus_tag="ACHKBL_002134"
     CDS             complement(17297..18628)
                     /locus_tag="ACHKBL_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO5819010.1"
                     /translation="MQPSRNFDDLKFSSIHRRILLWGSGGPFLDGYVLVMIGVALEQL
                     TPALKLDADWIGLLGAGTLAGLFVGTSLFGYISDKVGRRKMFLIDIIAIGVISVATMF
                     VSSPVELLVMRVLIGIVIGADYPIATSMITEFSSTRQRAFSISFIAAMWYVGATCADL
                     VGYWLYDVEGGWRWMLGSAAIPCLLILIGRFELPESPRWLLRKGRVKECEEMMIKLFG
                     EPVAFDEEQPQQTRFRDLFNRRHFPFVLFVAAIWTCQVIPMFAIYTFGPQIVGLLGLG
                     VGKNAALGNVVISLFFMLGCIPPMLWLNTAGRRPLLIGSFAMMTLALAVLGLIPDMGI
                     WLVVMAFAVYAFFSGGPGNLQWLYPNELFPTDIRASAVGVIMSLSRIGTIVSTWALPI
                     FINNYGISNTMLMGAGISLFGLLISVAFAPETRGMSLAQTSNMTIRGQRMG"
     gene            complement(18687..18974)
                     /gene="fixX"
                     /locus_tag="ACHKBL_002135"
     CDS             complement(18687..18974)
                     /gene="fixX"
                     /locus_tag="ACHKBL_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein FixX"
                     /protein_id="HFO5819011.1"
                     /translation="MTSPVNVDVKLGVNKFNVDEEHPHIVVKADADKQALELLVKACP
                     AGLYKKQDDGSVRFDYAGCLECGTCRILGLGSALEQWEYPRGTFGVEFRYG"
     gene            complement(18971..20257)
                     /locus_tag="ACHKBL_002136"
     CDS             complement(18971..20257)
                     /locus_tag="ACHKBL_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFO5819012.1"
                     /translation="MSEDIFDAIIVGAGLAGSVAALVLAREGAQVLVIERGNSAGAKN
                     VTGGRLYAHSLEHIIPGFADSAPVERLITHEKLAFMTEKSAMTMDYCNGDETSPSQRS
                     YSVLRSKFDAWLMEQAEEAGAQLITGIRVDNLVQRDGKVVGVEADGDVIEAKTVILAD
                     GVNSILAEKLGMAKRVKPTDVAVGVKELIELPKSVIEDRFQLQGNQGAACLFAGSPTD
                     GLMGGGFLYTNENTLSLGLVCGLHHLHDAKKSVPQMLEDFKQHPAVAPLIAGGKLVEY
                     SAHVVPEAGINMLPELVGDGVLIAGDAAGMCMNLGFTIRGMDLAIAAGEAAAKTVLSA
                     MKSDDFSKQKLAEYRQHLESGPLRDMRMYQKLPAFLDNPRMFSGYPELAVGVARDLFT
                     IDGSAPELMRKKILRHGKKVGFINLIKDGMKGVTVL"
     gene            complement(20308..21249)
                     /locus_tag="ACHKBL_002137"
     CDS             complement(20308..21249)
                     /locus_tag="ACHKBL_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HFO5819013.1"
                     /translation="MNTFSQVWVFSDTPSRLPELMNGAQALANQINTFVLNDADGAQA
                     IQLGANHVWKLSGKPDERMIEDYAGVMADTIRQHGADGLVLLPNTRRGKLLAAKLGYR
                     LNAAVSNDASAVSVQDGKATVKHMVYGGLAIGEERIATPYAVLTISSGTFDVAQPDAS
                     RTGETHTVEWQAPAVAITRTATQARQSNSVDLDKARLVVSVGRGIGSKENIALAEQLC
                     KAIGAELACSRPVAENEKWMEHERYVGISNLMLKPELYLAVGISGQIQHMVGANASQT
                     IFAINKDKNAPIFQYADYGIVGDAVKILPALTAALAR"
     gene            complement(21264..22034)
                     /locus_tag="ACHKBL_002138"
     CDS             complement(21264..22034)
                     /locus_tag="ACHKBL_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein FixA"
                     /protein_id="HFO5819014.1"
                     /translation="MKIITCYKCVPDEQDIAVNNADGSLDFSKADAKISQYDLNAIEA
                     ACQLKQQAAEAQVTALSVGGKALTNAKGRKDVLSRGPDELIVVIDDQFEQALPQQTAS
                     VLAAAAQKAGFDLILCGDGSSDLYAQQVGLLVGEILNIPAVNGVSKIISLTADTLTVE
                     RELEDETETLSIPLPAVVAVSTDINSPQIPSMKAILGAAKKPVQVWSAADIGFNAEAA
                     WSEQQVAAPKQRERQRIVIEGDGEEQIAAFAENLRKVI"
BASE COUNT         5422 a         6187 c         5813 g         4957 t
ORIGIN      
        1 gaaaccaggc tataatcaag cctggttttt tattggattt tcagcgtggc gcaggcaggt
       61 tttatcttaa cccgacactg gcgggatacc ccgcaaggaa ccgaagtttc cttttggtta
      121 gcgacggaca acgggccgtt gcaggttacg cttgcgccgc aagagtccgt ggcgtttatt
      181 cccgccgatc aggttccccg cgcccagcat attttgcagg gtgagcaagg ctttcgcctg
      241 acgccgctgg cgttaaagga ttttcatcgc cagccggtgt atggccttta ctgtcgcgcc
      301 catcgccagt tgatgaatta cgaaaagcgc ctgcgtgaag gtggcgttac cgtctacgag
      361 gccgatgtgc gcccgccaga acgctatctg atggagcggt ttatcacctc accggtatgg
      421 gtcgagggtg atatacgcaa tggcgctatc gttaatgccc gtctgaaacc gcatcccgac
      481 taccgtccgc cgctcaagtg ggtttctatt gatattgaaa ccacccgcca cggtgagctg
      541 tactgcatcg gcctggaagg ttgcgggcag cgcatcgttt atatgctggg gccagagaat
      601 ggcgacgcct ccgcgctcga ttttgaactg gagtacgtcg ccagccgccc gcagctactg
      661 gaaaaactca acgcctggtt tgcgactcac gatcccgatg tgatcatcgg ctggaacgtg
      721 gtgcagttcg atctgcgaat gctgcaaaaa catgccgagc gttaccgtat tccgctgcgg
      781 ctggggcgcg ataacagcga gctggagtgg cgcgagcacg gctttaaaaa cggtgtgttt
      841 tttgctcagg ttaaaggtcg gctaattatc gacggtatcg aggcactgaa atccgcgttc
      901 tggaatttct cttcattctc gctggaaacc gtcgctcagg agttattagg cgaaggaaaa
      961 tctatcgata acccgtggga tcgaatggac gaaattgacc gccgtttcgc cgaagataag
     1021 cctgcgctgg caacttataa cctgaaagat tgcgagctgg tgacgcagat cttccacaaa
     1081 actgaaatca tgccgttttt gctcgaacgg gcgacggtga acggcctgcc ggtagatcga
     1141 cacggcggtt cggtggcagc gtttggtcat ctctattttc cccgtatgca tcgcgctggt
     1201 tatgtcgcgc ctaatctcgg cgaagtgccg ccgcacgcca gccctggcgg ctacgtgatg
     1261 gattcgcgac ctgggcttta tgattcggtg ctggtgctgg actacaaaag cctgtacccg
     1321 tcgatcatcc gcacctttct gattgatccc gtcgggctgg tggaaggcat ggcgcagcct
     1381 gatccagagc acagcaccga aggctttctc gatgcctggt tctcgcgaga aaaacattgc
     1441 ctgccggaga ttgtgactaa catctggcac gggcgcgatg aagccaaacg tcagggtaat
     1501 aagccgcttt cgcaggcgct gaagatcatt atgaatgcct tttatggcgt gcttggcacc
     1561 accgcctgcc gcttcttcga tccgcgcctg gcgtcgtcga tcactatgcg tggtcatcag
     1621 atcatgcggc aaaccaaagc gttgattgaa gcgcagggct acgacgtgat ctacggcgat
     1681 accgactcaa cgtttgtctg gttgaaaggc gcacattcgg aagaagaagc ggcgaaaatt
     1741 ggtcgtgcac tggtgcagca cgttaacgcc tggtgggcag aaacgctgca aaaacaacgg
     1801 ctaaccagcg cactggaact ggagtatgaa acccatttct gccgttttct gatgccaacc
     1861 attcgcggag ccgataccgg cagcaaaaag cgctacgccg gactgattca ggagggcgac
     1921 aaacagcgga tggtatttaa agggctggaa accgtgcgca ccgactggac gccgctggcc
     1981 cagcagtttc agcaggagct gtacctgcgt atcttccgca acgagccata tcaggaatac
     2041 gtgcgcgaaa ccatcgacaa gctgatggcg ggcgaactgg atgcgcggct ggtttaccgt
     2101 aaacgccttc gccgtccgct gagcgaatat cagcgtaatg ttccgcctca tgtacgcgcc
     2161 gctcgccttg ccgatgaaga aaaccaaaag cgcggtcgcc ccttgcaata tcagaaccgc
     2221 ggctccatta agtacgtatg gaccaccaac ggcccggagc cgctggacta ccaacgttca
     2281 ccgctggatt acgaacacta tctgacccgc cagctacaac ccgtggcgga gggaatactc
     2341 ccttttattg aggataattt tgctacactt atgaccgggc aacttgggct attttgagca
     2401 aaaaaagagt tcgccagata ccattttgat gcgtgacgaa tgctttgcca tccagtacca
     2461 tagcgccctt tccattcctg gacctgaata acaccactac ctcataagca cggtagcggg
     2521 tggttattgc ctgcaattaa agatatagag ccgaacacat atgcctttta cacttggtca
     2581 acgctggatc agcgatacag aaagcgaatt gggacttgga accgttgtcg cggtggatgc
     2641 gcgaactgtc actttacttt tcccatctac tggtgaaaac cgtctgtacg cacgcagtga
     2701 ttcccccgtg acccgcgtga tgttcaaccc tggtgatact attaccagcc atgacggctg
     2761 gcagatgcaa gtcgaagaag taaaagaaga aaatggcttg ctgacctata tcggtactcg
     2821 cctggatact gaagagtccg gcgtagccct gcgtgaagtt ttccttgata gcaaactggt
     2881 gttcagcaaa ccgcaggacc gtctgtttgc cgggcagatt gaccgtatgg accgctttgc
     2941 gctgcgttat cgcgcgcgta agtattccag cgaacagttc cgtatgccgt acagcggcct
     3001 gcgcggtcag cgtaccagcc tgatcccgca ccagctcaac atcgctcatg atgttggccg
     3061 ccgccacgcg ccgcgcgtcc tgctggctga cgaagtgggt ttagggaaaa ccattgaagc
     3121 cgggatgatc ctgcatcagc aactgctctc tggcgctgct gaacgtgtgc tgattatcgt
     3181 cccggaaacc ttacagcatc agtggctggt agaaatgctg cgccgtttca acctgcgctt
     3241 tgctctgttt gatgatgagc gttatgccga agctcagcac gatgcttaca acccgttcga
     3301 taccgaacag ctggtgattt gctcgctgga ttttgctcgt cgtagcaaac agcgtctgga
     3361 acatctctgt gaagccgaat gggatctgct ggtggtcgat gaagcccatc acctggtgtg
     3421 gagcgaagat gcgccgagcc gcgaatatca ggccattgaa caactggcag agcacgtgcc
     3481 gggcgttctg ctgctgaccg caaccccgga acagctgggg atggaaagcc acttcgcccg
     3541 tctgcgtctg ctggacccga accgtttcca cgattttgcg caattcgttg aagagcagaa
     3601 aaattatcgt ccggttgcgg acgccgttgc catgctgctg gcaggtaaca aactgagcaa
     3661 tgatgaactg aacatgctcg gtgagatgat cggcgagcag gatatcgagc cgctgttgca
     3721 agcagcaaac agcgacagcg aagatgccca gagcgcccgt caggaactgg tttcgatgct
     3781 gatggatcgc cacggcacca gccgcgtgct gttccgtaac acgcgtaacg gtgtgaaagg
     3841 cttcccgaaa cgcgagctgc atactattaa gctgccactg ccgacgcagt atcagaccgc
     3901 tattaaagtc tccggcatta tgggcgcacg taaaagtgcg gaagatcgcg ctcgcgatat
     3961 gctctacccg gagcgtattt atcaggaatt tgaaggcgat aacgccacct ggtggaactt
     4021 cgatccgcgc gttgaatggc tgatgggcta cctgaccagc catcgctctc agaaagtgct
     4081 ggtgatctgc gccaaagctg ccactgcgct gcaactggag caagtactgc gcgaacgtga
     4141 aggtatccgc gctgctgtgt tccacgaagg catgtcgatt atcgaacgtg accgcgctgc
     4201 cgcctggttt gctgaagaag acaccggtgc gcaggtactg ctgtgctcgg aaatcggttc
     4261 tgaaggacgt aacttccagt tcgccagcca catggtgatg tttgacctgc cattcaaccc
     4321 ggatctgctg gagcagcgta ttggtcgtct ggatcgtatc ggtcaggcgc acgatattca
     4381 gatccatgtg ccttatctgg aaaaaactgc tcagtcggtg ctggtgcgct ggtatcacga
     4441 aggtctggat gcattcgagc acacctgccc gaccggacgc actatttacg atagtgtata
     4501 caacgatctg attaactatc tggcttcacc ggatcaaacc gaaggctttg acgatctgat
     4561 caaaaactgc cgcgagcaac atgaagcgct gaaagcacag ctggaacaag gtcgtgaccg
     4621 cctgctggaa atccattcca acggtggcga aaaagcccag gcactggcag aaagcattga
     4681 agagcaggat gacgatacca acctgatcgc cttcgccatg aacctgttcg atattatcgg
     4741 tatcaatcag gacgatcgcg gcgacaacat gatcgtgctg acgccgtccg atcatatgct
     4801 ggtgccggac ttccctggcc tgtcggaaga tggcatcacc atcaccttcg accgtgaagt
     4861 ggcgctggcg cgtgaagatg cacagtttat tacctgggag catccgctga tccgcaacgg
     4921 tctggatctg atcctttctg gcgataccgg tagcagcacg atttcactgt tgaaaaacaa
     4981 agcgttgccg gtaggtacgc tgttggtgga actgatttat gtggttgaag cccaggctcc
     5041 gaagcagttg cagctcaacc gcttcctgcc accgacgccg gtacgtatgc tgctggataa
     5101 aaacggcaac aacctggcgg cgcaggtaga gtttgaaacc tttaaccgcc agcttaacgc
     5161 ggttaaccgt cacaccggca gcaagctggt taacgccgtg cagcaggatg ttcacgctat
     5221 ccttcagctg ggggaagcgc agatcgagaa atctgcccgt gcattgattg atgccgcacg
     5281 taacgaagcc gacgaaaaac tgtctgccga gctgtctcgt ctggaagcgc tgcgtgcagt
     5341 taacccgaac attcgtgacg acgaactgac cgccattgag agcaaccgtc agcaggtaat
     5401 ggaaagcctg gatcaggcag gctggcgtct ggatgccctg cgtttgatcg ttgtgacgca
     5461 tcagtaacgg agccgaagat ggggatggaa aactacaatc cgccgcagga accctggctg
     5521 gttgtcctgt atcaggatga ccatattatg gtggtcaaca agccgagcgg tttgttgtct
     5581 gtgccgggtc gtctggaaga gcacaaagac agcgtgatga cgcgcattca gcgtgattat
     5641 ccgcaggcag aatcggtgca tcgtctggat atggctacca gcggcgtgat tgtagtggcg
     5701 ctgaccaaag ccgcggagcg ggagttaaaa cgccagttcc gcgagcgcga gcctaaaaaa
     5761 cagtatgtgg cccgcgtctg ggggcatcca tcccctgcgg aaggtctggt ggatctgccg
     5821 ctgatttgcg actggccaaa ccgcccgaaa cagaaagtct gttacgaaac aggtaaacct
     5881 gcgcagacgg agtatgaagt ggtggaatat gcggcggata acacggcaag agtggtgtta
     5941 aaaccgatta ccgggcgttc gcatcagctg cgtgtgcata tgctggcgct gggtcatccg
     6001 attctcggcg atcgttttta tgcatcacca gaagcgagag caatggcacc acgtttgttg
     6061 ctacatgcag agatgctgac gattacccat ccggcgtatg gcaatagtat gacgtttaaa
     6121 gcaccagcgg atttttaata gtcacgctgg cgaagttcgg taggccggag aaggcgttca
     6181 cgccgcatcc ggcaattttg cgccgattgc ctgatgcgac gcgggccgcg tcttatcagg
     6241 cctacacgtt gaaacttatt taaacccttt ctgcagctta atcaattcat aggcctgctg
     6301 aatttcctgc gctttttgct tcgccatctc catcatctca ggcggcaaac ctttcgccac
     6361 cagcttatcg ggatggtgtt cactcatcag cttacggtag gcacgtttga tggtggtcgc
     6421 atcatccgtc ggcttcacgc ccagcacatt acaggcatct tccagcgttg ggccacgctg
     6481 cgcttgctgc cagttaccac cgccagattg ctgctgataa ccgccgccaa actgtgcacc
     6541 gccctgcatc atgcgcaaaa actggtcaaa ctgggcgcgg gagatcccta attcttcagc
     6601 aatgacatac agcacagccc gttcattcgg gtgcagtgaa ccatcagcaa acgccgcctg
     6661 aatctggatc tccagaaaca tacgaattaa gtcaaaacga ccaaagcaga cactgcgaaa
     6721 ctggcgcatc ttttcgcgca gcgggtaatt gtctgatttt cccacccgga acgcgttttg
     6781 cgccgcagta cgggaagcgc catgaaggtt cattcggtcc atcaactggc tggcgatatg
     6841 aatatcagcc tccgtgacgc gacctttgga tttggttaaa tgccccatca cttcaaaagt
     6901 ggtggcaaaa aacagcgcct gacgctcacg ctggttggcg aaccacgcca ttttacggct
     6961 acgggcttta tcaaacatat ggccaattaa caggcctaac actacgcccc aaaagccgcc
     7021 gcccatcagt aaggccacgg ccacgccaat gatttttccc caatactgca tatattcccc
     7081 aaatcgacac acggatatca gggctatctc ccacaatata aaggtgcttt taccgttttc
     7141 cggctgcggt caattgtggg acatcgccta taatttgcat tatcatacct gtcattcaat
     7201 gccgtgccta acaccactga cgctattcgg acaggattaa cactagcgta gagatgacga
     7261 gtacgttagt ctctgaccgt ttgtcacgcg caacgttacc gatgatggaa caataaaatc
     7321 aacgtatgaa aaaacgtatc cccactctcc tggccaccat gattgccacc gccctttata
     7381 gtcaacaggg actggcagcc gacctcgcct cacagtgcat gttgggcgtg ccaagctatg
     7441 accgtcctct ggtacagggc gataccaatg acttacccgt gactatcaat gctgaccacg
     7501 cgaaagggga ctacccggat gacgccgtgt ttactggcag cgtggatatc atgcagggta
     7561 acagccgtct gcaggccgac gaagtgcagc tccatcaaaa agaggcgcca ggacaaccag
     7621 agccggtacg taccgttgat gcgctcggta atgtccatta cgacgataac caggtgatcc
     7681 tcaaagggcc gaaaggctgg gcgaatctga acaccaaaga taccaacgtc tgggagggtg
     7741 attaccagat ggtgggtcgc cagggtcgcg gtaaagcgga cctgatgaaa caacgtggcg
     7801 aaaaccgcta taccattctg gataacggta gctttacctc ctgtctgccg ggttctgaca
     7861 cctggagcgt ggtaggcagc gaaattattc atgaccgcga agaacaagtt gcggagatct
     7921 ggaacgcccg ctttaaggtg ggtccggtac cgatctttta tagcccctat ttgcagttgc
     7981 cggtgggtga caaacgtcgc tctggtttct tgatcccgaa cgccaagtac accaccacca
     8041 actactttga gttctacctg ccatattact ggaacatcgc gccaaatatg gatgccacca
     8101 tcacgccgca ttatatgcat cgtcgtggca atatcatgtg ggagaacgaa ttccgctacc
     8161 tctcccaggc gggcgctggc ttgatggaac tggactatct gccttcagat aaagtctatg
     8221 aagatgaaca cccgaacgat gacagttcac gtcgttggtt attctactgg aaccactccg
     8281 gggtcatgga tcaggtgtgg cgtttcaacg tcgactacac caaggtcagc gatcctagct
     8341 acttcaatga ttttgataac aagtacggtt ccagtactga cggctacgca acgcaaaaat
     8401 tcagcgttgg ctatgcggtg caaaacttca atgccaccgt ttcaaccaag cagttccagg
     8461 tcttcagcga gcagaacacc agtagctact cggcagagcc gcagttagac gttaactact
     8521 accagaatga tgttggtccg tttgatacac gcatttacgg ccaggcagtg cactttgtta
     8581 acaccagaga cgacatgcct gaagcaaccc gtgttcacct ggaaccgacc atcaatttgc
     8641 cgctctctaa taactggggc agcatcaata ccgaagcgaa gttgctggca acccattatc
     8701 agcaaactaa tcttgactgg tataactcca gaaacacaac caagctggac gaatccgtta
     8761 accgcgtaat gccgcaattc aaagttgacg gcaaaatggt ctttgaacgc gatatggaaa
     8821 tgctggcgcc gggttatacc caaacgctgg aaccgcgcgc gcagtattta tacgtgccgt
     8881 atcgcgatca gagcgacatc tataactacg actcgtctct gctgcaatct gactactctg
     8941 gcctgttccg ggaccggact tacggcggtc ttgaccgtat tgcctccgct aaccaggtga
     9001 cgaccggtgt cacatctcgc atatatgatg atgctgccgt tgaacgtttt aatatttccg
     9061 ttggtcaaat ctactatttc acggagtctc gcactggcga tgacaacata acatgggaga
     9121 atgacgacaa aacgggctca ctggtgtggg caggcgatac ttactggcgt atctcagagc
     9181 gttggggatt gcgtggcgga attcagtacg atacacgtct ggataacgta gcgaccagta
     9241 actccagcat tgaataccgt cgggatgaag accgtctggt acagctgaat taccgttacg
     9301 ccagcccgga atatattcag gctacgctgc ctaagtacta ttccactgct gagcaatata
     9361 agaatggtat ttcgcaggta ggtgccgtcg ccagctggcc aattgccgat cgttggtcaa
     9421 ttgttggggc ctactactac gacaccaatg cgaacaagca agccgactct atgttaggtg
     9481 tgcaatacag ctcctgctgc tatgcaattc gcgtcggtta cgagcggaag ctgaacggtt
     9541 gggataacga taaacaacat gcggtatatg acaacgcaat cggctttaac atcgaacttc
     9601 gcggcctgag ctccaactac ggtctgggta cgcaagagat gctgcgttcg aacattctgc
     9661 cgtatcaaaa cactttgtga tttgttgatt taccacgtaa tccgcagtgc ggttaattga
     9721 aatggaaaaa gtatgaagaa ctggaaaacg ctgcttctcg gtatcgccat gatcgcgaat
     9781 accagtttcg ctgcccccca ggtagtcgat aaagtcgcag ccgtcgtcaa taacggcgtc
     9841 gtgctggaaa gcgacgttga tggattaatg caatcggtaa aactgaacgc tgctcaggca
     9901 aggcagcagc ttcctgatga cgcgacgctg cgccaccaaa tcatggaacg tttgatcatg
     9961 gatcaaatca tcctgcagat ggggcagaaa atgggagtga aaatctccga tgagcagctg
    10021 gatcaggcga ttgctaacat cgcgaaacag aacaacatga cgctggatca gatgcgcagc
    10081 cgtctggctt acgatggtct gaactacaac acctatcgta accagatccg caaagagatg
    10141 attatctctg aagtgcgtaa caacgaggtg cgtcgtcgca tcaccatcct gccgcaggaa
    10201 gtcgaatccc tggcgcagca ggtgggtaac caaaacgacg ccagcactga gctgaacctg
    10261 agccacatcc tgatcccgct gccggaaaac ccgacctctg atcaagtgaa cgaagcggaa
    10321 agccaggcgc gtgccattgt cgatcaggcg cgtaacggcg ctgatttcgg taagctggcg
    10381 attgctcatt ctgccgacca gcaggcgctg aacggcggcc agatgggctg gggccgtatt
    10441 caggagttgc cggggatctt cgcccaggca ttaagcaccg cgaagaaagg cgacattgtt
    10501 ggcccgattc gttccggcgt tggcttccat attctgaaag ttaacgacct gcgcggcgaa
    10561 agcaaaaata tctcggtgac cgaagttcat gctcgccata ttctgctgaa accgtcgccg
    10621 atcatgactg acgaacaggc ccgcgtgaaa ctggaacaga ttgctgccga tatcaagagt
    10681 ggtaaaacga cttttgctgc cgcagcgaaa gagttctctc aggatccagg ctctgctaac
    10741 cagggcggtg atctcggttg ggctacacca gatattttcg atccggcctt ccgtgacgcc
    10801 ctgacccgcc tgaacaaagg tcaaatgagt gcaccggttc actcttcatt cggctggcat
    10861 ttaatcgaac tgctggatac ccgtaatgtc gataaaaccg acgctgcgca gaaagatcgt
    10921 gcataccgca tgctgatgaa ccgtaagttc tcggaagaag cagcaagctg gatgcaggaa
    10981 caacgtgcca gcgcctacgt taaaatcctg agcaactaat ggttaaaacc caacgtgttg
    11041 tgatcactcc cggcgagccc gccgggattg gcccggactt agttgtccag cttgcacagc
    11101 gtgagtggcc ggtcgaactg gttgtttgtg ccgatgccac tctccttacc gaccgggcag
    11161 cgatgctcgg tttgccgctc accctccgtc cttattcccc caactcccct gcacaaccgc
    11221 aaactgcggg cacattaacg ctacttcctg tcgcgctacg tgaatctgtc actgcggggc
    11281 agttagcggt tgaaaatggg cattacgtag tggagacgct ggcgcgagcg tgtgatggct
    11341 gtctgaacgg tgaatttgct gcgctgatca caggccccgt gcataaaggc gtcattaacg
    11401 acgcaggcat tccgtttacc ggtcataccg agtttttcga agagcgttcg caggcgaaaa
    11461 aagtggtgat gatgctggcg accgaagaac ttcgcgtggc gctggcaacg acgcatttac
    11521 cgctgcgcga tatcgcagat gctatcaccc ctgcgctttt gcacgaagtg attgctattt
    11581 tgcatcacga tttgcggacc aaatttggta ttgccgaacc gcgcattctg gtctgcgggc
    11641 tgaatccgca cgcgggcgaa ggcggtcata tgggtacgga agagatagac accattattc
    11701 cggtgctcga caagctgcgg gcgcagggga tgaaactcaa cgggccgctg cctgccgata
    11761 ccctgtttca gccgaaatat cttgataacg ccgacgcggt gctggcgatg taccacgatc
    11821 agggtcttcc cgtgctaaaa taccagggct tcgggcgcgg tgtgaacatt acgctgggcc
    11881 tgccctttat tcgcacatca gtggaccacg gtaccgcgct tgaactggcg ggacgtggca
    11941 aagccgatgt cggcagtttt attacggcgc ttaatctcgc catcaaaatg attgttaaca
    12001 cccaatgaat aatcgagtcc accagggcca cttagcccgt aaacgcttcg ggcaaaactt
    12061 tctcaacgat cagttcgtga tcgacagcat tgtttctgcc attaacccgc agaagggcca
    12121 ggcgatggtc gaaatcggcc ccggtctggc ggcattgacc gaaccggtcg gcgaacgtct
    12181 ggataagctc acggtcatcg aacttgaccg cgatctggcg gcacgtctgc aaacgcatcc
    12241 attcttaggc ccgaaactga ctatttatca gcaggatgcg atgaccttta actttggtga
    12301 actggccgag aaaatgggtc agccgctgcg tgttttcggc aacctgcctt ataacatctc
    12361 cacgccgttg atgttccatc tgtttagcta tactgatgcc attgccgaca tgcactttat
    12421 gttgcaaaaa gaggtggtga atcgtctggt tgcaggaccg aacagcaagg cgtatggtcg
    12481 attaagcgtc atggcgcaat actattgcaa cgtgatcccg gtgctggaag taccgccgtc
    12541 agcctttaca ccaccaccca aagtggattc cgccgtcgtg cgcctggttc ctcatgcaac
    12601 gatgcctcac ccggttaaag atgttcgcgt gttgagccgc atcaccaccg aagcctttaa
    12661 ccagcgtcgt aaaaccattc gtaacagcct cggcaacctg tttagcgtcg aggtgttaac
    12721 gggaatgggg atcgacccgg cgatgcgagc ggaaaatatc tctgtcgcgc aatattgcca
    12781 gatggcgaac tatctggcgg agaacgcgcc tttgcaggag agttaacgat gatcaattcg
    12841 ccccgagtgt gtattcaggt tcaaagcgtc tacattgagg cacaatcttc acctgataat
    12901 gaacgttacg tttttgctta taccgtaacc atacgcaatc tggggcgagc gccagtgcag
    12961 ttgttggggc gttactggct gatcaccaat ggcaatggcc gtgaaaccga agtccagggc
    13021 gaaggagtgg ttggcgtcca gccacttatc gcacctggcg aagagtacca gtacaccagc
    13081 ggtgcaatca ttgaaacccc gctgggcacc atgcagggtc actacgaaat gatcgatgaa
    13141 aatggcgtcc ctttcagcat cgacattccc gtattccgac tcgccgttcc cacactcatt
    13201 cattaaaaga atatggcgac ataccttatt ggcgacgttc atggttgtta cgatgaactg
    13261 atcgcattgc tgcataaagt agaatttacc cctgggaaag ataccctctg gctgacgggc
    13321 gatctggtcg cgcgcggccc gggttcgctg gatgttctgc gctatgtgaa atccttaggc
    13381 gacagcgtac gtctggtgct gggcaatcac gatctgcatc tgctggcggt atttgccggg
    13441 atcagccgca ataaaccgaa agatcgcctg acaccgctgc tggaagcgcc ggatgccgac
    13501 gagctgctta actggctgcg tcgccagcct ctgctgcaaa tcgacgaaga gaaaaagctg
    13561 gtgatggccc acgccgggat cacgccacag tgggatctac agaccgccaa agagtgcgcg
    13621 cgcgatgtag aagcggtgct gtcgagcgac tcctacccct tcttccttga tgccatgtac
    13681 ggcgatatgc caaataactg gtcaccggaa ttgcgggggc tgggaagact gcggtttatc
    13741 accaacgcct ttacccgtat gcgtttttgc ttcccgaacg gtcaactgga tatgtacagc
    13801 aaagaatcgc cggaagaggc ccctgcccca ctgaaaccgt ggtttgcgat tcctggccct
    13861 gtcgctgaag agtacagcat cgcctttggt cactgggcat cgctggaagg caaaggtacg
    13921 ccggaaggta tttacgcgct ggatactggc tgctgctggg gtggtacatt aacctgcctg
    13981 cgctgggaag ataaacacta ttttgtccag ccgtcgaacc ggcataagga tttgggcgaa
    14041 gcggcggcgt cttaaacaca gcctgataca ggaaggccgg ataagacgcg accggcgtcg
    14101 catccggcac tagccgtaaa ttctatacaa acttaccgcc gctccagaat ctcaaagcaa
    14161 tagctgtgag agttctgcgc atcagcgtcg tggaattcgc tgaataccga ttcccagtca
    14221 tccggctcgt aatccgggaa atgggtgtcg ccttccactt ctgcgtcgat atgcgtcaga
    14281 tacagttttt gcgctttggg caggaactgc tcataaacgc gaccgccgcc aatcaccatg
    14341 atttctggta cgtcaccaca cgccgcaatg gcttcatcca ccgacttcac ccacgttacg
    14401 cgatcgtccg tacccggttg actgctgagg ataatatttt tgcgtcctgg caacggacga
    14461 ccgatggatt cccaggtatg gcggcccata atcacgggtt tatttaaagt gttgcgttta
    14521 aaccaggcga gatcggcagg caggttccac ggcatggcgt tttccatgcc gataacacga
    14581 tctaccgcta acgccgcaat cagactgatc attgagattt cccgataaaa aaaattgtcg
    14641 ccactatacg taaagcgtaa accgtcgtcg actggtgcga ggatgatgtt gaggaaaatt
    14701 ttatattctg ctggcgagtc cacgctctcc cactggactc gccgcattac aatgaaataa
    14761 aaacaattag ttagctgtaa agtgtgattt acgtcactct ttattaggat gagggtttcg
    14821 tttccggttc atccgccatg ttgccggtat gtttaccttc ttcggttccc tgccagccat
    14881 gtcgttgaat taatgacaga tgttcgcggt cctcggtaat gatctcactt aacatcgcgc
    14941 tggtgcgttt atagaccgcc gcgcgggctt tggtgtcgtt ctcaaccatt gccatctctt
    15001 ccaccatctg aatattaaag cggcggaaca catcggcgcg ttctcgcgct tcatacggcc
    15061 ccagacctaa actttccagt gccagacgcc cggttttcag cgcaccttca aaggtttcac
    15121 gctccggctt ttcaacgcct gcctgacgca aacgaatgta gtggtcgaca tcgcgggcgc
    15181 gggcaataat ctgcaaatgc gggaaatgtt ctttcaccat ctccgtcagt tgcaggttgg
    15241 tttgcggatc gtcgatagcg ttaatcagca cttccgcttt cgccgctccg gcagattcca
    15301 gtaaatccat ccgcgtggca tcgccataaa acactttcat accaaattta cgcaaggttt
    15361 cgatatggtc cggatcgtga tcgagcacca ccattttcac cccgctggag agcagtaaac
    15421 gtccggtgat ctgcccaaaa cgaccgaatc cggcgatgat tacccgcggc tgttcttcgt
    15481 cgatctcatc ggcttcacgc gcctcttcac tagaagattg ctcaaggcga ttgaggatca
    15541 ccagcagaat cggcgttgcc gccatcgaca gcgccaccgc cagggtcagc gatttcgccc
    15601 actccggctc cagcacattc gccatctgcg ccgcaccaaa tactacaaag gcaaactcac
    15661 taccctgccc taatagcacc gcaaaccagc gacgctgttt atttggcacc tgcaacggcc
    15721 gggcaatcag ccacagcatg gcgattttga tgatgaggaa accgagcagc aaaatgacaa
    15781 tgcgcaacgg attttcaagc agcgtgccaa agtctatcga catgccaacg ccgatgaaaa
    15841 acagccccag cagcaaacct ttaaatggtt cgatatcgct ttccagcgca tgacggtatt
    15901 ccgagcttgc cagcagtacg cccgccagaa acgcgcccat cgccatcgac aagccgacct
    15961 cttccagcag caaaccaaag ccaaacacga ggaataacgc cacggcacta aacacttccc
    16021 gcaagccaga gcgggcaaca aaacgcagtg ccggacgcgt gacatagcgc cccagcaata
    16081 ccaccagcac cagcgcaccc gccactttta acgccgagag agcaaatgcg cccatcgtcg
    16141 tcgaggcact gctcgccgcc agtagcggga tcatcgccac cagcgggatc gccgcgatat
    16201 cctggaacag cagcaccgca aaggcactgc gacccatttg cgtcaccatc agattgcgtt
    16261 cattcatcgc ctgcatggca atcgccgtag aggagagcgc cagcgtcatg ccgatcaatt
    16321 ccgcgacctg ccagcgcagc ccaagcaaca tacagaacag ccccagcagg ccgccgcaaa
    16381 tcaccatctg caatgcgccg ccgccgaaca ccgccgcacg cagcttccac agcctttgtg
    16441 gatcgagttc gaggccgata ataaacagca tcagcaccac gccaatctcg gcaaagtgca
    16501 gaatagattc ggcatcggtc accagtcgca gcccccacgg gccaataatg cagccggcga
    16561 tcaggtagcc aagtaccgct cccagaccga gacgtaccgc aatgggaaca atcagcgccg
    16621 ccgaaccgag ataaatcagc gcctgaataa gcgtatggct atccatgatg tgcctcctgc
    16681 cattccagca gacgttgctt atagtgccgc gcctgccctt cgagggtttc gtcgtcacaa
    16741 ataaaggtgc agtgcatggc aaacggtggc agccagttca gcccgcagta gattgccgtc
    16801 gcctgtagcg gctgtgacag cacatcaaag cccggatgcg caccaatctc aaaatggctt
    16861 tccccaccgc cggtcgtcac cgcccacagt aaatgtttgc catgcagcgc cgtgccgccg
    16921 tgaccgtaag cccagccgtg cgagaagact ttatcgatcc aaagtttaag aagcggagga
    16981 atgctgtacc actgcatcgg gtgctgccag acgatcagat cggcgcgaga cagcgcctcc
    17041 tgctcggcgg caatatcgat attgaagtca ggatagagtt gataaagaga gcgaatttcg
    17101 acgccttcca gcgtccttgc ctgttcaagc atccgtttat tcgcatggga atgatgcgga
    17161 tacggatgcg cataaattat aagaatcata ggtagcccgc gtgattacgc ctttgttttc
    17221 ccacaaagtg tagtcatata ttcacgcggc ttatagtcaa taatattgat tcagaaaaga
    17281 gagaaatctg aacaaattac cccattctct gcccgcggat cgtcatattg ctggtctgcg
    17341 ccagtgacat ccctcgagtc tccggggcaa acgctacgga aatcaacaag ccaaacagcg
    17401 agatacccgc ccccattagc atcgtgttac tgataccgta attattgata aagatcggca
    17461 gtgcccaggt cgaaacaata gtgccaatac ggcttaagga cataatcacg cccacggcag
    17521 aggcgcggat atccgtcggg aagagttcat taggatagag ccactgcaaa ttacccggcc
    17581 cgccagagaa aaaggcatac accgcaaatg ccatcactac cagccagatc cccatatccg
    17641 ggatcagccc caaaaccgcc agcgccagcg tcatcatggc aaagctgcca atcaacaatg
    17701 gacgccgtcc ggcagtgttt agccacagca tcggtggaat acagccgagc ataaagaaca
    17761 ggctaatcac cacgttcccc agtgccgcgt ttttgccaac acccaatccc aacaaaccaa
    17821 cgatttgcgg gccaaaggtg tagatggcga acatcgggat cacctggcag gtccagatgg
    17881 cggcaacaaa cagaacaaaa ggaaaatggc ggcgattaaa cagatcgcga aaacgggttt
    17941 gctgcggctg ctcttcatcg aaagccaccg gttcgccaaa cagtttgatc atcatctctt
    18001 cgcactcttt tactcgccct ttgcgtaaca accagcgggg agattcaggc agttcgaatc
    18061 gaccaatcaa aatcaacaaa caggggatcg ctgcgctacc cagcatccag cgccaaccac
    18121 cttccacatc ataaagccag taaccgacca gatcggcgca ggtcgcgccg acataccaca
    18181 tcgcggcaat aaagctgatg gaaaacgccc gctgacgggt actggagaac tcagtgatca
    18241 tcgaagtggc gatgggataa tctgcaccga tgacaatgcc gatgagtacc cgcatcacta
    18301 acagttcgac gggggatgag acaaacatcg tcgccaccga tatcacgccg atggcgatga
    18361 tatcaatgag gaacattttg cgccgtccga ctttatcgga gatatagcca aacagcgatg
    18421 tgccaacgaa cagcccggcg agcgttcccg cgcccagcaa gccaatccag tcagcgtcca
    18481 gtttcagcgc cggagtcagt tgctccagcg ccacgccaat cattaccagt acataaccat
    18541 ccagaaacgg accaccgctt ccccacagca aaatgcggcg gtgaatagag gagaatttaa
    18601 gatcatcaaa gtttctggac ggttgcatgg gttattcctg tttttttgca atgagagtca
    18661 gtacggcatt acgtatcaaa ccaacatcag ccgtaacgga actccacacc aaaggtgccg
    18721 cgcgggtatt cccactgttc cagcgccgac cccagcccca gaatgcgaca ggtgccgcac
    18781 tccagacatc cggcgtaatc gaagcgcaca ctgccgtcat cctgcttctt gtacagacct
    18841 gcggggcacg ctttcaccag cagctccagg gcctgtttat cggcatcggc cttcacaacg
    18901 atgtgcggat gctcttcatc gacattgaat ttattgacgc ccagtttgac gtccacattg
    18961 acgggagaag tcataaaacg gtcactcctt tcatgccatc cttgatcaga ttgatgaagc
    19021 ccactttctt gccgtggcgg aggattttct tgcgcatcag ttccggcgca ctgccgtcaa
    19081 tggtgaacag gtcacgcgcc acgcccaccg ccagttccgg gtagccgcta aacatgcgtg
    19141 ggttatcaag gaacgccggt agtttctggt acatgcgcat atcgcgcagc gggccactct
    19201 caagatgctg acgatattcc gcgagttttt gcttactgaa atcgtcgctt ttcatcgctg
    19261 aaagcacagt ttttgctgct gcttccccgg cggcaatcgc cagatccata ccgcgaatgg
    19321 taaaaccgag gttcatacac attccggcag catcaccggc aatcaatacg ccgtcaccaa
    19381 ccaactccgg cagcatgttg atacctgctt ccggcactac gtgagcggaa tattccacca
    19441 gcttgccgcc cgcgatcagc ggtgcaacgg ccggatgttg tttgaaatct tccagcattt
    19501 gcggcaccga tttttttgcg tcatgcagat gatgcaaacc acaaaccagc cccagcgaca
    19561 gggtgttttc attggtataa agaaagccgc cgcccatcag gccatcagtg ggggaacccg
    19621 caaacaggca agccgcaccc tgattaccct gcaactgaaa acggtcttcg ataaccgact
    19681 tcggtaattc gatcagttcc ttcacgccaa ccgccacatc cgtcggttta acgcgttttg
    19741 ccatccccag cttttcggca aggatggagt tcaccccgtc agcaaggatc accgttttcg
    19801 cttcaattac atcgccatcg gcttctacgc cgacaacttt gccatcgcgc tgtacgaggt
    19861 tatcgacacg aatcccggta attaactgcg cacccgcttc ttcggcctgc tccatcagcc
    19921 aggcatcaaa tttactgcgc aaaacggagt aagaacgctg ggatggtgag gtttcgtcac
    19981 cgttgcagta gtccatagtc atcgcagact tttccgtcat aaacgcgagt ttttcatggg
    20041 tgatcaggcg ttctacgggg gcggagtcag cgaaaccagg aataatgtgt tccagactgt
    20101 gggcatagag acgcccgccg gtgacgttct tggcaccagc ggaattgcct cgctcgataa
    20161 ctaacacttg cgccccttcg cgggcgagca ccagtgcggc aaccgaaccg gcaagccctg
    20221 caccgacgat gatggcgtca aagatatctt cggacataag agctccctgt cagcggcagg
    20281 gccaaaatgc agccctgcca gagtggatca acgcgctaaa gctgcggtca gtgccggaag
    20341 gatcttcacg gcgtcgccaa caatgccgta atccgcgtac tggaagatcg gcgcattttt
    20401 atctttgttg atggcgaaaa tggtttgcga cgcattagcg ccaaccatgt gctggatctg
    20461 cccggagatc cccaccgcca ggtacagttc aggtttcagc atcaggttgg agataccgac
    20521 atagcgttcg tgctccatcc atttttcgtt ttccgccacc ggacgagaac aggccaactc
    20581 cgcacctatc gccttgcaaa gctgttctgc cagcgcaatg ttctctttgc tgccaatacc
    20641 gcgaccaacg ctgaccacca ggcgggcttt gtcgagatcg acgctgttgc tctgacgcgc
    20701 ctgggttgcc gtgcgggtaa tcgccacagc cggagcctgc cactccacgg tgtgcgtttc
    20761 gccagtgcgt gacgcgtctg gctgagccac atcgaacgtg ccgctgctga tggtcagaac
    20821 cgcatacggc gtggcaatgc gttcttcgcc aatcgccaga ccgccgtaaa ccatgtgttt
    20881 cactgtcgct ttaccgtcct gcacgctaac agcgctggca tcgttagaca ccgccgcgtt
    20941 aaggcggtaa cccagtttcg ccgccagtaa tttgccgcga cgggtgtttg gcaacagcac
    21001 cagaccgtct gcgccgtgct ggcgaatagt gtcagccatg acaccggcgt aatcttcgat
    21061 catccgctcg tccggtttgc cgcttaattt ccagacatga ttagcgccga gctggattgc
    21121 ctgtgcgccg tcggcatcat tgaggacgaa cgtgttgatt tgattagcta aagcctgcgc
    21181 accgttcatc agttccggca gacgagaagg ggtatcgctg aatacccaga cttgagaaaa
    21241 cgtgttcata gcatcccctg taattaaatg actttgcgaa gattttcagc aaatgcggcg
    21301 atctgttctt cgccgtcgcc ttcaatcacg atgcgctgac gctcgcgctg tttcggcgcg
    21361 gcaacctgtt gttctgacca ggctgcctct gcgttaaaac cgatatccgc cgccgaccat
    21421 acctggactg gctttttcgc cgcgccgaga atggctttca tcgaaggaat ttgtggggag
    21481 ttgatatcag tggaaacagc aacaaccgca ggcagcggaa tgcttaaggt ttctgtttca
    21541 tcttccagtt cgcgctcaac ggtgagggta tctgcagtca gggagataat tttgctgacg
    21601 ccgttaactg ccggaatatt aaggatttcg cctaccagca aaccaacctg ctgggcataa
    21661 aggtcggaag aaccatcgcc acagaggatc aaatcaaagc ctgctttctg agcggctgca
    21721 gccagtacgc tcgccgtttg ttgcggcagt gcctgctcga actggtcatc aatcaccaca
    21781 atcagttcat ccggaccgcg cgatagcaca tctttacgcc ctttggcgtt ggtcagggct
    21841 ttaccgccca cacttaaggc cgtcacctgc gcctctgctg cctgttgctt tagctggcaa
    21901 gccgcttcaa tagcgttgag atcgtattgg cttattttgg catcggcttt gctgaagtct
    21961 aacgaaccat cagcattatt gaccgcaata tcctgttcat caggcacgca cttatagcaa
    22021 gtaataatct tcattgcatc tccagaaatc atgaaggaaa cgtcgttcat atcggtggtg
    22081 ttaattacag atatcacgcc ctttaatttg gaaataaaac tttaatcacc aatattgaaa
    22141 atgtcacacg cagataaatc caactttcaa tattgttaag ctcctcacca atattgaaaa
    22201 catggcgtag aaaaaagaaa ttttcaatat tgttttatgg atcaccaata ttgaaagtca
    22261 gatgctattt ttttactcac aacagaacat aacaaactga ttaataatca aaattaacga
    22321 aaaaatgccc tctattcacc cgaaacaaaa atgtgataac aatcacagaa tgcagctta
//
LOCUS       DAZFLD010000066        22121 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.066, whole genome shotgun sequence.
ACCESSION   DAZFLD010000066 DAZFLD010000000
VERSION     DAZFLD010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22121)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22121)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22121
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.066"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..773)
                     /locus_tag="ACHKBL_002139"
     CDS             complement(<1..773)
                     /locus_tag="ACHKBL_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6531 domain-containing protein"
                     /protein_id="HFO5819015.1"
                     /translation="MSGKPAARQGDMTQYGGSIVQGSAGVRIGAPTGVACSVCPGGVT
                     SGHPVNPLLGAKVLPGETDLALPGPLPFILSRTYSSYRTKTPAPVGSLGPGWKMPADI
                     RLQLRDNTLILSDNGGRSLYFEHLFPGEDGYSRSESLWLVRGGVAKLDEGHRLAALWQ
                     ALPEELRLSPHRYLATNSPQGPWWVLGWCERVPEADEVLPAPLPPYRILTGLVDRFGR
                     TQTFHREAGGEFSGEITGVTDGAGRHFRLVLTTQALRAE"
     gene            complement(976..1377)
                     /gene="nikR"
                     /locus_tag="ACHKBL_002140"
     CDS             complement(976..1377)
                     /gene="nikR"
                     /locus_tag="ACHKBL_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010045 - response to nickel cation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HFO5819016.1"
                     /translation="MQRVTITLDDDLLETLDSLSQRRGYNNRSEAIRDILRSALAQEA
                     TQQHGTQGFAVLSYVYEHEKRDLASRIVSTQHHHHDLSVATLHVHINHDDCLEIAVLK
                     GDMGDVQHFADDVIAQRGVRHGHLQCLPKED"
     gene            complement(1382..2188)
                     /gene="nikE"
                     /locus_tag="ACHKBL_002141"
     CDS             complement(1382..2188)
                     /gene="nikE"
                     /locus_tag="ACHKBL_002141"
                     /EC_number="7.2.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikE"
                     /protein_id="HFO5819017.1"
                     /translation="MTLLNVCDLSHHYAHGGFSGKHQHQAVLNNVSLTLKSGETVALL
                     GRSGCGKSTLARLLVGLESPSQGNISWRGEPLAKLNRAQRKAFRRDIQMVFQDSISAV
                     NPRKTVREILREPMRHLLSLKKAEQLARASEMLKAVDLDDSVLDKRPPQLSGGQLQRV
                     CLARALAVEPKLLILDEAVSNLDLVLQAGVIRLLKKLQQQFGTACLFITHDLRLVERF
                     CQRVMVMDNGQIVETQVVGDKLTFSSDAGRVLQNAVLPAFPVRRRTTEKV"
     gene            complement(2185..2949)
                     /gene="nikD"
                     /locus_tag="ACHKBL_002142"
     CDS             complement(2185..2949)
                     /gene="nikD"
                     /locus_tag="ACHKBL_002142"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikD"
                     /protein_id="HFO5819018.1"
                     /translation="MPQQIELRNIALQAAQPLVHGVSLTLKRGRVLALVGGSGSGKSL
                     TCAATLGILPAGVRQTAGEILADGKPVSPCALRGIKIATIMQNPRSAFNPLHTMHTHA
                     RETCLALGKPADDATLTAAIEAVGLENTARVLKLYPFEMSGGMLQRMMIAMAVLCESP
                     FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSHGK
                     IVEQGDVETLFNAPKHTVTRSLVSAHLALYGMELAS"
     gene            complement(2949..3782)
                     /gene="nikC"
                     /locus_tag="ACHKBL_002143"
     CDS             complement(2949..3782)
                     /gene="nikC"
                     /locus_tag="ACHKBL_002143"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015099 - nickel cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikC"
                     /protein_id="HFO5819019.1"
                     /translation="MNFFLSSRWSVRLALIIIALLALIALTSQWWLPYDPQAIDLPSR
                     LLSPDAQHWLGTDHLGRDIFSRLMAATRVSLGSVMACLLLVLTLGLIIGGSAGLIGGR
                     VDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIALSHWAWYARMVRSLVISL
                     RQREFVLASRLSGAGHVRVFIDHLAGAVIPSLLVLATLDIGHMMLHVAGMSFLGLGVT
                     APTAEWGVMINDARQYIWTQPLQMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHA
                     H"
     gene            complement(3779..4723)
                     /gene="nikB"
                     /locus_tag="ACHKBL_002144"
     CDS             complement(3779..4723)
                     /gene="nikB"
                     /locus_tag="ACHKBL_002144"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikB"
                     /protein_id="HFO5819020.1"
                     /translation="MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPALDYLRLSNL
                     PPTPEMLASTRTMLGLDQPLYVQYGTWLWKALHLDFGISFASQRPVLDDMLNFLPATL
                     ELAGAALVLILLTSVPLGIWAARHRDRLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSV
                     YLQWLPAMGYGGWQHIILPAVSIAFMSLAINARLLRASMLDVAGQRHVTWARLRGLND
                     KQTERRHILRNASLPMITAVGMHIGELIGGTMIIENIFAWPGVGRYAVSAIFNRDYPV
                     IQCFTLMMVVVFVVCNLIVDLLNAALDPRIRRHEGAHA"
     gene            complement(4723..6297)
                     /gene="nikA"
                     /locus_tag="ACHKBL_002145"
     CDS             complement(4723..6297)
                     /gene="nikA"
                     /locus_tag="ACHKBL_002145"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO5819021.1"
                     /translation="MLSTLRRTLFALLACASFIVHAAAPDEITTAWPVNVGPLNPHLY
                     TPNQMFAQSMVYEPLVKYQADGSVIPWLAKSWTHSEDGKTWTFTLRDDVKFSNGEPFD
                     AEAAAENFRAVLDNRQRHAWLELANQIVDVKALSKNELQITLKSAYYPFLQELALPRP
                     FRFIAPSQFKNHETMNGIKAPIGTGPWVLQESKLNQYDVFVRNENYWGEKPAIKKITF
                     NVIPDPTTRAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPIETVMLALN
                     TAKAPTNELAVREALNYAVNKKSLIDNALYGTQQVADTLFAPSVPYANLGLKPRQYDP
                     QKAKALLEKAGWTLPAGKDIREKNGQPLRIELSFIGTDALSKSMAEIIQADMRQIGAD
                     VSLIGEEESSIYARQRDGRFGMIFHRTWGAPYDPHAFLSSMRVPSHADFQAQQGLADK
                     PLIDKEIGEVLATHDETQRQALYRDILTRLHDEAVYLPISYISMMVVSKPELGNIPYA
                     PIATEIPFEQIKPVKP"
     gene            complement(6408..6995)
                     /gene="acpT"
                     /locus_tag="ACHKBL_002146"
     CDS             complement(6408..6995)
                     /gene="acpT"
                     /locus_tag="ACHKBL_002146"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase AcpT"
                     /protein_id="HFO5819022.1"
                     /translation="MYRIVLGKVSTLSAAPLPPALREQAPQGPRRERWLAGRALLSHT
                     LSPLPEIIYGEQGKPAFAPETPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRANWR
                     WLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSS
                     LSVSHCQLDNLSLAICTPTPFTLTADSVQWIDSVN"
     gene            complement(7050..8099)
                     /locus_tag="ACHKBL_002147"
     CDS             complement(7050..8099)
                     /locus_tag="ACHKBL_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFO5819023.1"
                     /translation="METPQPDKTGMHILLKLASLVVILAGIHAAADIIVQLLLALFFA
                     IVLNPLVTWFIRRGVQRPVAITIVVVVMLIALTALVGVLAASFNEFISMLPKFNKELT
                     RKLFKLQEMLPFLNLHMSPERMLQRMDSEKVVTFTTALMTGLSGAMASVLLLVMTVVF
                     MLFEVRHVPYKMRFALNNPQIHIAGLHRALKGVSHYLALKTLLSLWTGVIVWLGLELM
                     GVQFALMWAVLAFLLNYVPNIGAVISAVPPMIQVLLFNGIYECILVGALFLVVHMVIG
                     NILEPRMMGHRLGMSTMVVFLSLLIWGWLLGPVGMLLSVPLTSVCKIWMETTKGGSKL
                     AILLGPGRPKSRLPG"
     gene            8231..9448
                     /locus_tag="ACHKBL_002148"
     CDS             8231..9448
                     /locus_tag="ACHKBL_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO5819024.1"
                     /translation="MPEPVAEPALNGLRLNLRIVSIVMFNFASYLTIGLPLAVLPGYV
                     HDVMGFSAFWAGLVISLQYFATLLSRPHAGRYADLLGPKKIVVFGLCGCFLSGLGYLT
                     AGLTASLPVISLLLLCLGRVILGIGQSFAGTGSTLWGVGVVGSLHIGRVISWNGIVTY
                     GAMAMGAPLGVVFYHWGGLQALALIIMGVALVAILLAIPRPTVKASKGKPLPFRAVLG
                     RVWLYGMALALASAGFGVIATFITLFYDAKGWDGAAFALTLFSCAFVGTRLLFPNGIN
                     RIGGLNVAMICFSVEIIGLLLVGVATMPWMAKIGVLLAGAGFSLVFPALGVVAVKAVP
                     QQNQGAALATYTVFMDLSLGVTGPLAGLVMSWAGVPVIYLAAAGLVAIALLLTWRLKK
                     RPPEHVPEAASSS"
     gene            complement(9452..10009)
                     /gene="dcrB"
                     /locus_tag="ACHKBL_002149"
     CDS             complement(9452..10009)
                     /gene="dcrB"
                     /locus_tag="ACHKBL_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage sensitivity protein DcrB"
                     /protein_id="HFO5819025.1"
                     /translation="MRNLVKYVGIGLLVMGLAACDDKDTNATAQGSVAESNATGNPVN
                     LLDGKLSFSLPADMTDQSGKLGTQANNMHVWSDATGQKAVIVIMGDDPKEDLAVLAKR
                     LEDQQRSRDPQLQVVTNKAIELKGHKMQQLDSIISAKGQTAYSSVILGNVGNQLLTMQ
                     ITLPADNQQKAQTTAENIINTLVIQ"
     gene            complement(10082..10747)
                     /locus_tag="ACHKBL_002150"
     CDS             complement(10082..10747)
                     /locus_tag="ACHKBL_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HFO5819026.1"
                     /translation="MNVFSQTQRYKALFWLSLFHLLVITSSNYLVQLPVSIFGFHTTW
                     GAFSFPFIFLATDLTVRIFGAPLARRIIFAVMIPALLISYVISSLFYMGSWQGFGALA
                     HFNLFVARIATASFMAYALGQILDVHVFNRLRQSRHWWLAPTASTLFGNVSDTLAFFF
                     IAFWRSPDAFMAEHWMEIALVDYCFKVLISIVFFLPMYGVLLNMLLKRLADKSEINAL
                     QAS"
     gene            10969..11214
                     /gene="tusA"
                     /locus_tag="ACHKBL_002151"
     CDS             10969..11214
                     /gene="tusA"
                     /locus_tag="ACHKBL_002151"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097163 - sulfur carrier activity
                     [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HFO5819027.1"
                     /translation="MTDLFSSPDHTLDALGLRCPEPVMMVRKTVRNMQPGETLLIIAD
                     DPATTRDIPGFCTFMEHELVAKETDELPYRYLIRKGG"
     gene            complement(11316..13514)
                     /gene="zntA"
                     /locus_tag="ACHKBL_002152"
     CDS             complement(11316..13514)
                     /gene="zntA"
                     /locus_tag="ACHKBL_002152"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase
                     ZntA"
                     /protein_id="HFO5819028.1"
                     /translation="MSTPDNHGKKAPQFAAFKPLTTVQNANDCCCDGACSSTPTLSEN
                     VSGTRYSWKVSGMDCAACARKVENAVRQLAGVNQVQVLFATEKLVVDADNDIRAQVES
                     AVQKAGYSLRDEQSADEPQASRLKENLPLITLIVMMAISWGLEQFNHPFGQLAFIATT
                     LVGLYPIARQALRLIKSGSYFAIETLMSVAAIGALFIGATAEAAMVLLLFLIGERLEG
                     WAASRARQGVSALMALKPETATRLRNGEREEVAINSLRPGDVIEVAAGGRLPADGKLL
                     SPFASFDESALTGESIPVERATGDKVPAGATSVDRLVMLEVLSEPGASAIDRILKLIE
                     EAEERRAPIERFIDRFSRIYTPAIMAVALLVTLVPPLLFAASWQEWIYKGLTLLLIGC
                     PCALVISTPAAITSGLAAAARRGALIKGGAALEQLGRVTQVAFDKTGTLTVGKPRVTA
                     IHPATGISESELLTLAAAVEQGATHPLAQAIVREAQVAELAIPAAQSQRALVGSGIEA
                     QVNGERVLICAAGKHPADAFAGLINELESAGQTVVLVVRNDDVLGVIALQDTLRADAA
                     TAISELNALGVKGVILTGDNPRAAAAIAGELGLEFKAGLLPEDKVKAVTELNQHAPLA
                     MVGDGINDAPAMKAAAIGIAMGSGTDVALETADAALTHNHLRGLVQMIELARATHANI
                     RQNITIALGLKGIFLVTTLLGMTGLWLAVLADTGATVLVTANALRLLRRR"
     gene            complement(13588..14214)
                     /locus_tag="ACHKBL_002153"
     CDS             complement(13588..14214)
                     /locus_tag="ACHKBL_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HFO5819029.1"
                     /translation="MLWSFIAVCLSAWLSVDASYRGPTWQRWVFKPLTLLLLLLLAWQ
                     APMFDAISYLVLAGLCASLLGDALTLLPRQRLMYAIGAFFLSHLLYTIYFASQMTLSF
                     FWPLPLVLLVLGALLLAIIWTRLEEYRWPICTFIGMTLVMVWLAGELWFFRPTAPALS
                     AFVGASLLFISNFVWLGSHYRRRFRADNAIAAACYFAGHFLIVRSLYL"
     gene            14355..14714
                     /locus_tag="ACHKBL_002154"
     CDS             14355..14714
                     /locus_tag="ACHKBL_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HFO5819030.1"
                     /translation="MSKPPLFFIVIIGLIVVAASFRFMQQRREKADNDMAPLQQKLVV
                     VSNKREKPINDRRSRQQEVTPAGTSMRYEASFKPQSGGMEQTFRLDAQQYHALTVGDI
                     GTLSYKGTRFVSFVGEQ"
     gene            complement(14717..14986)
                     /locus_tag="ACHKBL_002155"
     CDS             complement(14717..14986)
                     /locus_tag="ACHKBL_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1145 family protein"
                     /protein_id="HFO5819031.1"
                     /translation="MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMH
                     GMQLALLKSTLPKDGPQMTTAEKVRIFLFGVFELLVWQKKFKVKK"
     gene            complement(14976..15572)
                     /gene="rsmD"
                     /locus_tag="ACHKBL_002156"
     CDS             complement(14976..15572)
                     /gene="rsmD"
                     /locus_tag="ACHKBL_002156"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase"
                     /protein_id="HFO5819032.1"
                     /translation="MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLF
                     NWLAPVIVDAQCLDCFAGSGALGLEALSRYAAGATLIEMDRAVSQQLIKNLATLKAGN
                     ARVVNSNAMSFLAQKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADEALIYVESEV
                     ENGLPTVPANWSLHREKVAGQVAYRLYQREAQGESDAD"
     gene            15722..17218
                     /gene="ftsY"
                     /locus_tag="ACHKBL_002157"
     CDS             15722..17218
                     /gene="ftsY"
                     /locus_tag="ACHKBL_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HFO5819033.1"
                     /translation="MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEP
                     AKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVVESEKAQPEAEVVAQPELVVEETP
                     EPVAIEREELPLPEDVNAEEVSPEEWQAEAETVEIVEAAEEEAAKEEITDEELEAQAL
                     AAEAAEEAVMVVPPAEEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISL
                     FRGKKIDDDLFEELEEQLLIADVGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEM
                     GEILAKVDEPLNVEGKTPFVILMVGVNGVGKTTTIGKLARQFEQQGKSVMLAAGDTFR
                     AAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKS
                     HLMEELKKIVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLD
                     GTAKGGVIFSVADQFGIPIRYIGVGERIEDLRPFKADDFIEALFARED"
     gene            17221..17889
                     /gene="ftsE"
                     /locus_tag="ACHKBL_002158"
     CDS             17221..17889
                     /gene="ftsE"
                     /locus_tag="ACHKBL_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HFO5819034.1"
                     /translation="MIRFEHVSKAYLGGRQALQGVTFHMQPGEMAFLTGHSGAGKSTL
                     LKLICGIERPSAGKIWFSGHDITRLKNREVPFLRRQIGMIFQDHHLLMDRTVYDNVAI
                     PLIIAGASGDDIRRRVSAALDKVGLLDKAKNFPIQLSGGEQQRVGIARAVVNKPAVLL
                     ADEPTGNLDDALSEGILRLFEEFNRVGVTVLMATHDINLISRRSYRMLTLSDGHLHGG
                     VGHE"
     gene            17882..18940
                     /gene="ftsX"
                     /locus_tag="ACHKBL_002159"
     CDS             17882..18940
                     /gene="ftsX"
                     /locus_tag="ACHKBL_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HFO5819035.1"
                     /translation="MNKRDAINHIRQFGGRLDRFRKSVGGSGDGGRNAPKRAKSSPKP
                     VNRKTNVFNEQVRYAFHGALQDLKSKPFATFLTVMVIAISLTLPSVCYMVYKNVNQAA
                     TQYYPSPQITVYLQKTLDDDAAAGVVAQLQAEQGVEKVNYLSREDALGEFRNWSGFGG
                     ALDMLEENPLPAVAVVIPKLDFQGTESLNTLRDRITQINGIDEVRMDDSWFARLAALT
                     GLVGRVSAMIGVLMVAAVFLVIGNSVRLSIFARRDSINVQKLIGATDGFILRPFLYGG
                     ALLGFSGALLSLILSEILVLRLSSAVAEVAQVFGTKFDINGLSFDECLLLLLVCSMIG
                     WVAAWLATVQHLRHFTPE"
     gene            19185..20039
                     /gene="rpoH"
                     /locus_tag="ACHKBL_002160"
     CDS             19185..20039
                     /gene="rpoH"
                     /locus_tag="ACHKBL_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HFO5819036.1"
                     /translation="MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYH
                     GDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLV
                     SFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
                     LGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWE
                     EQAANRLTDAMQGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMK
                     KLRAAIEA"
     gene            20311..21414
                     /gene="livJ"
                     /locus_tag="ACHKBL_002161"
     CDS             20311..21414
                     /gene="livJ"
                     /locus_tag="ACHKBL_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     substrate-binding protein LivJ"
                     /protein_id="HFO5819037.1"
                     /translation="MNIKGKALLAGCIALAFSNMALAEDIKVAVVGAMSGPVAQYGDQ
                     EFTGAEQAVADINAKGGIKGNKLQIVKYDDACDPKQAVAVANKVVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMITPAATAPELTARGYQLILRTTGLDSDQGPTAAKYILEKVK
                     PQRIAIVHDKQQYGEGLARAVQDGLKKGNANVVFFDGITAGEKDFSTLVARLKKENID
                     FVYYGGYHPEMGQILRQARAAGLKTQFMGPEGVANVSLSNIAGESAEGLLVTKPKNYD
                     QVPANKPIVDAIKAKKQDPSGAFVWTTYAALQSLQVGLNQSDDPAEIAKYLKANSVDT
                     VMGPLTWDEKGDLKGFEFGVFDWHANGTATDAK"
     gene            complement(21602..21985)
                     /gene="panM"
                     /locus_tag="ACHKBL_002162"
     CDS             complement(21602..21985)
                     /gene="panM"
                     /locus_tag="ACHKBL_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0031638 - zymogen activation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase autocleavage activator
                     PanM"
                     /protein_id="HFO5819038.1"
                     /translation="MKLTIIRLENFSDQDRIDLQKIWPEYSPSSLQVDDNHRIYAARF
                     NERLLAAVRVTLSGTEGALDSLRVREVTRRRGVGQYLLEEVLRNNPGVSCWWMADAGV
                     EDRGVMTAFMQALGFTAQQGGWEKR"
BASE COUNT         5237 a         5860 c         6134 g         4890 t
ORIGIN      
        1 tcttctgccc gcagcgcctg cgtggtcagt accagccgga agtgacggcc tgcgccgtcg
       61 gtcacgccgg tgatttcgcc gctgaattcg ccgccagcct cacggtgaaa cgtctgtgtg
      121 cgcccgaagc ggtccaccag cccggtcagt atccggtacg gcggcagcgg cgcaggcagc
      181 acctcatccg cctccggcac ccgctcacac cagccgagta cccaccacgg cccctgcgga
      241 ctgttcgtcg ccagataacg atgcggactc aggcgaagct cttccggcag cgcctgccag
      301 agtgcggcca gccggtgacc ttcatccagt ttcgccacgc cgccgcgcac cagccagagt
      361 gactcgctgc ggctgtaacc gtcctcaccg ggaaacaggt gctcaaaata caggcttctg
      421 ccgccgttat cactgagtat cagtgtgtta tcgcgcagct gtaagcggat atccgcaggc
      481 attttccagc cggggccgag gctccccacc ggcgcgggcg tttttgtccg gtaactgctg
      541 taggtgcggg agagaatgaa cggcagcggg ccgggcaggg caaggtcggt ttcgccggga
      601 aggaccttcg caccgagcag cggattgacc ggatgaccgg acgtcacccc gccggggcac
      661 accgaacagg ccacaccggt gggggcacca atacgtaccc cggcagaacc ctgaacaatg
      721 ctaccgccat actgcgtcat gtcgccctga cgcgccgccg gttttccgct catcgtaaaa
      781 tccttttcta ttattttaat gacagtatct tcatacgaaa aagcgaagaa aaacttgtgg
      841 tcacaatgaa ttgaagattt ttaggaatat atttagaacg ttacaaaaga gaaggttcgg
      901 atagggtatt tagagaggaa tcaggtgtgc tagtggccct ttgttgatcg ttcaccaaag
      961 gacccaataa taggcttaat cttccttcgg caagcactgc aaatgcccgt gccgcacgcc
     1021 gcgctgggcg ataacgtcat cggcaaaatg ctgcacgtcg cccatatcgc ctttcagcac
     1081 ggcgatttcc agacagtcgt cgtgattgat gtggacatgt agcgtcgcga cggagagatc
     1141 gtggtgatgg tgctgggtgg agacaatacg gctggctaag tcgcgttttt cgtgttcata
     1201 cacataggag agcaccgcga aaccttgcgt gccgtgctgc tgggtagctt cctgcgccag
     1261 ggcgctacgc agaatatcgc ggatagcttc ggaacggttg ttatagccac gacgctggct
     1321 caggctgtcc agcgtctcca gtaaatcgtc atcaagcgtg atggtaactc gttgcataga
     1381 cttaaacctt ttctgtggtg cgacggcgca cggggaatgc gggtaatacc gcgttttgta
     1441 gcacacgtcc ggcgtcagag gaaaaggtta atttatctcc caccacctgg gtttcgacga
     1501 tttgtccgtt gtccatcacc atcacccgct gacagaagcg ttccaccagg cgtaagtcgt
     1561 gggtgatgaa caggcaggcg gtgccaaact gttgttgcag ctttttcagc aggcgaatga
     1621 cgcccgcctg taacacgaga tcaaggttag aaacggcttc atccagaatc agtagtttcg
     1681 gttcgaccgc cagcgcgcga gccaggcaga cgcgctggag ctgaccgccg cttaactgcg
     1741 gtgggcgttt gtctagaacg ctgtcatcga gatcgaccgc cttcagcatt tcgctggcac
     1801 gcgccagttg ttcggctttt ttcagcgata gcaggtggcg catcggttca cgcaggatct
     1861 cgcgcacggt ttttcgcgga ttcacggcgc tgatggagtc ctgaaatacc atctgaatat
     1921 cgcggcggaa cgctttacgc tgggcgcgat tgagctttgc caacggttcg ccgcgccagc
     1981 taatattccc ttgtgaggga gactctaagc ctaccagcag ccgcgcgaga gtgctttttc
     2041 cgcagccgct gcgccccagc agagcgacgg tttcgccgct tttcagggtc agggaaacgt
     2101 tattcagtac cgcctgatgc tgatgttttc cgctaaatcc accgtgcgca tagtgatgtg
     2161 aaaggtcgca gacgttaagt aaagtcatga tgccagctcc ataccgtaga gggcgagatg
     2221 agcggaaacc aggctgcgcg tcaccgtatg tttgggggcg ttaaacagcg tttctacatc
     2281 gccctgttcg acaattttac cgtgtgacat caccgccacg tcatccgcca gacgcgccac
     2341 aacgcccata tcatgggtga ccagcagcat tcccggtgct tgtttttgca taatgctttc
     2401 cagcagatcg aggatgcgcg cctgtgctac cacgtcgagg tcggtggtcg gttcatcggc
     2461 gatgataaac ggcgattcac acaacaccgc catcgcaatc atcatgcgct gcaacatgcc
     2521 gccgctcatc tcgaacgggt acagcttcaa cacgcgcgcg gtgttttcca accccaccgc
     2581 ttctatggca gcggtaagcg tagcgtcatc ggcgggtttc cccagcgcca ggcaggtttc
     2641 acgcgcgtgg gtgtgcatgg tgtgtagcgg attaaaggcg ctgcgcgggt tctgcatgat
     2701 ggtggcaatt ttgatgccgc gcagggcaca gggagaaacc ggtttgccat cggctaaaat
     2761 ttcccccgcc gtctggcgaa cgcctgcggg cagaatgccc agcgtcgcgg cgcaggtcag
     2821 tgatttcccg ctgccgctac cgccgactaa cgccagcaca cgcccgcgtt taagggttaa
     2881 cgatacgccg tgcaccagcg gttgtgcggc ctgtagcgcg atattacgta gttcaatctg
     2941 ttgcggcatt agtgtgcgtg ctccgtcacc agatgaggat ccagatgatc gcgcagtgcg
     3001 tcacccacca ggttaaaggc catcacgctg ataaacagcg ccagccccgg ccagaacatt
     3061 tgcagcggct gggtccagat atactgacgc gcgtcgttaa tcatcacgcc ccattcggcg
     3121 gtcggcgcgg tcacgccgag accgaggaag gacatccccg cgacgtgtag catcatatga
     3181 ccgatatcca gcgtcgccag caccagcagc gaggggatca ccgcacctgc cagatgatca
     3241 ataaacaccc gcacatggcc cgcgccagaa agccgtgatg ccagcacaaa ctcgcgctgg
     3301 cgcagtgaaa tcaccaggct acgcaccatg cgtgcatacc acgcccagtg cgacagggcg
     3361 atggctataa tcacattggt caacccggtg ccgagcacgc cgaccataaa gaacgacaga
     3421 atcgaggttg ggaaggtcat aaacatatca gcgacgcgca tggtggcctg atcaacgcgc
     3481 ccgccaatca gcccggcgct gccgccaata atcagcccta atgtcagcac caacagcagg
     3541 caggccatta ccgagccgag cgacacgcgg gtcgccgcca tcagccgcga gaaaatatcg
     3601 cgccctaaat gatcggtacc cagccagtgc tgcgcatccg gtgaaagcag gcgcgacggc
     3661 agatcaatcg cctgggggtc atacggcagc caccactggc tggtgagcgc aatcagcgcc
     3721 agcagggcga taatgatcag cgccaggcgt accgaccagc gggaagagag gaaaaagttc
     3781 acgcgtgcgc tccttcatga cgacgaatgc gcgggtccag cgcggcgttg agtaaatcaa
     3841 caatcaaatt acagaccaca aaaaccacca ccatcatcag cgtaaagcac tggatcaccg
     3901 gatagtcacg gttaaaaatc gccgacaccg catagcgccc gacgcccggc caggcaaaga
     3961 tgttttcgat aatcatcgtt ccgccaatca gttcgccaat gtgcatcccc acggcggtga
     4021 tcatcggcag cgaggcattg cgcagaatat ggcggcgttc ggtctgtttg tcgttcaggc
     4081 cgcgtagccg agcccaggtg acgtgacgct gaccggcgac gtccagcata ctggcgcgca
     4141 gcagccgcgc gttaatcgcc agcgacataa aggcaatgga aaccgcaggc aaaatgatgt
     4201 gctgccagcc gccgtagccc atcgcgggta gccattgcag atataccgaa aacgccatca
     4261 ccagcaaaaa cgccagccag aagttgggca tcgacacgcc gagaaacgcg atgaaacgta
     4321 cggcgaaatc cggcagacga tcgcgatggc gcgccgccca gataccgagc ggtacggaag
     4381 tgagcagaat taacaccagc gcggcacctg caagttccag cgtggcgggc aggaagttca
     4441 gcatatcgtc cagcaccggg cgttggctgg cgaatgagat accaaagtca agatgcagcg
     4501 ccttccacaa ccaggtaccg tactggacgt acagcggctg atccagcccc agcatggtgc
     4561 gggtagaggc cagcatctcc ggcgtcggcg gcaggttaga taaacgcaga taatcgagcg
     4621 ccgggtcgcc ggtaccgagg cgcagcatca gaaaaatgat caccgaggcg gcgagcacca
     4681 tcgggatcag cagcagaaag cggcgtaata cgtaacgcaa cattaaggtt tcaccggttt
     4741 aatctgttcg aacggaattt cggtggcgat cggcgcgtag gggatgttac ccagctccgg
     4801 ttttgatacc accatcattg agatgtaact gataggcaga taaaccgcct cgtcatgcag
     4861 acgggtcaga atgtcgcgat acagcgcctg acgttgcgtt tcgtcatggg tcgccagcac
     4921 ttcgccgatc tctttatcaa tcagcggctt gtcggctaaa ccttgctgtg cctggaagtc
     4981 agcgtgcgac ggtacgcgca ttgaactcag gaaggcgtgt ggatcgtatg gcgcgcccca
     5041 ggtgcggtgg aaaatcatgc caaaacgacc gtcgcgctga cgagcataga tactgctctc
     5101 ttcttcgcca atcagcgaga catctgcgcc aatctggcgc atatcagcct gaatgatttc
     5161 cgccatcgat ttgcttaacg catcggtgcc gatgaacgac agttcaatgc gcagcggctg
     5221 accatttttc tcgcggatgt ctttgcctgc aggcagcgtc caaccggctt tttccagcaa
     5281 cgctttcgct ttctgcggat cgtactggcg cggtttcagg ccgaggttgg cgtagggcac
     5341 agaaggggca aacagggtgt cggcgacctg ctgggtacca tacaacgcgt tatcaatcag
     5401 cgattttttg ttcaccgcgt aattaagagc ttcacgtact gccagctcgt tggtgggggc
     5461 tttggcggta ttgagcgcca gcatcacggt ttcgatcggc tgtgacagtt gagtgtggta
     5521 agccgggttc tggctaaagc gggcgaaggt atcgagcggt aataaccctt cgtttccgta
     5581 cagcaggtcg atatcgccag tttcaaacgc caccgcacgg gtagtcgggt ccgggatcac
     5641 gttaaaggtg atctttttaa tcgctggctt ttcgccccag tagttttcgt tacggacgaa
     5701 gacatcgtac tgattcagtt tcgattcctg taaaacccac ggcccggtgc caatcggcgc
     5761 tttaattccg ttcatggttt cgtggttttt aaactgcgag ggtgcgataa aacggaaagg
     5821 acggggcagg gccagttctt gcaggaaagg atagtaggcg cttttcaggg tgatttgcag
     5881 ctcgttttta ctgagtgctt tgacatcaac aatctggttt gccagctcca gccaggcgtg
     5941 acgttgacgg ttatcgagca ctgcgcggaa gttttctgcc gccgcctctg catcaaacgg
     6001 ttcgccgttg gagaatttca cgtcatcacg cagggtgaag gtccaggttt taccatcttc
     6061 tgaatgagtc cagctttttg ccagccacgg gatcaccgaa ccgtctgcct gatatttcac
     6121 caatggttca taaaccatgc tctgggcgaa catctggtta ggcgtgtaaa ggtgcgggtt
     6181 tagtggcccg acattcaccg gccaggcggt ggtgatttca tctggtgcag cggcatggac
     6241 gataaaagac gcacaagcca gcagcgcaaa tagagtgcgg cggagtgtgg agagcatggt
     6301 aaccccaatg gattaaaata gatggcggaa ataagtatga cgattttaag tattcgtcat
     6361 actgattacc tgttctggat caatagatgg gcagtcgggt gggcggatca gttaactgaa
     6421 tcgatccatt gcacactgtc ggcggtgagc gtaaaggggg taggggtgca gatcgccagg
     6481 cttaaattgt ctagctgaca atggctgact gacagtgatg agtgataggt gctgtctacg
     6541 ctgacgattt gccaggcgct gccgccacgc tgtttaacga tggcttcttt gcgcgtccag
     6601 atgcgccaga acatttccag ttgctgatca ggatgcacgg cgtccatctc ggcatgttcc
     6661 ccaaggctga atacggcgtt cgccagccag cgccagttgg cgcgcgggcg aatcacttcg
     6721 atatcgcagc caacttcgcc ttcatcgctc aacagcaggg cgatatcgtc accgctatgg
     6781 cttaagttga accatagcgg cgtttccggc gcgaatgccg gtttgccttg ttcgccatag
     6841 atgatctccg gtagcgggga aagcgtgtgc gaaagcaatg cacgccccgc cagccagcgt
     6901 tcgcgtcgtg gaccttgcgg tgcttgctcg cgtaaagccg gtggcagtgg agctgcgctt
     6961 aaggtcgaaa ctttccccag aactatccga tacatatcag ggccaacgtt taatggaaaa
     7021 tgaaagtgcg tatcgtaaca cttgtcgcct catcccggta accgactttt cggtctgcct
     7081 ggccccagta aaatcgccag tttgctaccg cctttggtgg tttccatcca gattttacac
     7141 acgctggtta atggcaccga aagtagcatc cctaccgggc cgagcagcca tccccaaatt
     7201 aacaatgaaa gaaataccac catggtggac atccccagac gatggcccat catccgtggt
     7261 tctaaaatat tgccgatgac catatggacc actaaaaaca atgcgccgac cagaatacat
     7321 tcgtaaatac cattaaacag cagcacctga atcattggcg gtacggcgga aattaccgca
     7381 ccgatattgg gcacgtagtt gagcaaaaac gccagtaccg cccacatcag cgcaaactgt
     7441 acgcccataa gttccagccc cagccagacg ataacgcctg tccataaact tagcagtgtt
     7501 ttcaatgcca gataatgcga aacgccttta agtgcgcggt gtagtccggc gatgtgaatt
     7561 tgtggattat tcaacgcgaa acgcattttg taagggacgt ggcgcacttc aaacagcata
     7621 aaaactacgg tcatcaccag caaaagcacg ctcgccattg ccccggaaag cccggtcatt
     7681 agcgctgtgg tgaaggtaac cactttttcc gagtccatcc gctgcagcat tcgctccggt
     7741 gacatatgca aattaagaaa aggcaacatc tcctgcaatt taaaaagttt gcgcgtcagc
     7801 tccttattaa acttcggcag catagagata aattcgttaa acgatgccgc cagtacgccg
     7861 accagcgcag ttagtgcgat cagcatcacc accaccacaa tcgtaatggc aacggggcgt
     7921 tgtactcccc gacgaataaa ccaggtgacg agcgggttga ggacgatggc aaaaaacagc
     7981 gccagtaaca gctgcacaat gatatctgcc gctgcgtgaa tgcccgcgag gatcactacc
     8041 agcgaggcca gcttgagcag aatgtgcatg cccgttttat cgggttgagg ggtttccatt
     8101 ggggcttcct tgtgactttt tgtattaagt gtagcgggag tcacgccagc aatattctga
     8161 ttcttactgc tgattttcac gtcagcacgt ggtaaaaatg aaacactgtt gtaaaaatgt
     8221 ggtgatcctc atgcccgaac ccgtagccga acccgcgcta aacggattgc gcctgaattt
     8281 gcgcattgtc tccattgtca tgtttaactt cgccagctac ctcaccatcg ggttgccgct
     8341 cgctgtatta ccgggctatg tccatgatgt gatgggcttt agcgcttttt gggcaggatt
     8401 ggttatcagc ctgcaatatt tcgccacctt gctgagccgc cctcatgccg gacgttacgc
     8461 cgatttgctg ggacccaaaa agattgtcgt cttcggttta tgcggctgct ttttgagcgg
     8521 tctgggatat ctgacggcag gattaaccgc cagtctgccc gtcatcagcc tgttattact
     8581 gtgcctgggg cgcgtcatcc ttgggattgg gcaaagtttt gccggaacgg gatcgaccct
     8641 gtggggtgtt ggcgtggttg gctcgctgca tatcgggcgg gtgatttcgt ggaacggcat
     8701 tgtcacttac ggggcgatgg cgatgggtgc gccgttaggt gtcgtgtttt atcactgggg
     8761 cggcttgcag gcgttagcgt taatcattat gggcgtggcg ctggtggcca ttttgttggc
     8821 gatcccgcgt ccgacggtaa aagccagtaa aggcaaaccg ctgccgtttc gcgcggtgct
     8881 tgggcgcgtc tggctgtacg gtatggcgct ggcactggct tccgccggat ttggcgttat
     8941 cgccaccttt atcacgctgt tttatgacgc taaaggttgg gacggtgcgg ctttcgcgct
     9001 gacgctgttt agctgtgcgt ttgtcggtac gcgtttgtta ttccctaacg gcattaaccg
     9061 tatcggcggc ttaaacgtag cgatgatttg ctttagcgtt gagataatcg gcctgctact
     9121 ggttggcgtg gcgactatgc cgtggatggc gaaaatcggc gtcttactgg cgggggcagg
     9181 gttttcgctg gtgttcccgg cattgggcgt agtggcggta aaagcggttc cacagcaaaa
     9241 tcagggggcg gcgctggcaa cttacaccgt atttatggat ttatcgcttg gcgtgaccgg
     9301 accactggct gggctggtga tgagttgggc gggcgtcccg gtgatttatc tggcggcggc
     9361 gggactggtc gcaatcgcgt tattactgac gtggcgatta aaaaaacggc ctccggaaca
     9421 cgtccctgag gccgcctcat catcttaaaa cttactgaat aaccagcgta ttaatgatgt
     9481 tttctgcggt ggtctgcgct ttttgctgat tgtcagctgg cagcgtaatt tgcatggtca
     9541 gcagttgatt acccacgtta cccagaataa cggaagagta cgccgtctgg cctttcgcgg
     9601 agataatgct gtctaactgc tgcattttgt gacctttcag ctcaatggct ttattggtta
     9661 ccacttgcag ctgcggatcg cggctacgtt gctgatcttc cagacgcttc gccaacaccg
     9721 ccagatcttc tttcggatca tcgcccatga tgacgatgac tgctttttgc ccagtggcgt
     9781 cggaccagac atgcatgtta ttggcctgcg ttcccagctt accgctctgg tcggtcatat
     9841 ccgctggcag cgagaaactt aacttgccat caagcaggtt gacgggattc ccggtagcgt
     9901 tactttccgc gaccgacccc tgcgccgtag cgttagtgtc tttatcatca caggccgcaa
     9961 gccccataac cagcaggcca attccgacat atttaaccag attgcgcatt gacttcttcc
    10021 tttcgataaa cggccataac ggctcattca tccatcttat cacaactctg ataacgaacc
    10081 tttaactcgc ctgcaaagcg ttgatttcag atttatctgc cagtcttttc aacagcatat
    10141 tgagtaatac gccatacatt ggcaggaaga aaacgatact gattaacact ttgaaacagt
    10201 aatcgaccag cgcgatttcc atccagtgtt cagccataaa ggcatccggg ctacgccaga
    10261 aggcaatgaa gaagaaggcc agcgtgtcgc tgacgttacc gaacagtgtg gacgctgtcg
    10321 gtgccagcca ccagtgacga ctctgacgca ggcggttaaa aacgtgcacg tcgaggatct
    10381 gccccagcgc gtaggccatg aaactggcgg tggcgatacg ggcgacaaac aggttgaagt
    10441 gggcgagcgc gccgaatccc tgccaggaac ccatatagaa cagcgacgag atgacgtagg
    10501 agatcagtaa cgccgggatc atcaccgcaa agatgatacg acgggccagc ggtgcgccaa
    10561 aaatacgcac ggtcaggtcg gtagcaagga aaataaacgg aaagctaaat gcgccccagg
    10621 tggtatggaa accaaaaatg gagacgggaa gctgaaccag atagttactg gaggtgatca
    10681 ccagcagatg aaataacgat aaccagaaca acgccttata gcgttgagtt tgcgagaaaa
    10741 cgttcatatt gtaccttttt gattaaccat tggggtgagg gaacccaata cgtacgacac
    10801 gctattttac cttgattaaa taacgttctt accctttttt cgagccgccg catgatactg
    10861 ctttacgttg acaatgcaat ggttaatttt cacgcaatcg ttaacctggt ttgcttacgg
    10921 actcacaggg cgtaaactag cgccgttttt ttatgtgatg agaagaaaat gaccgatctc
    10981 ttttccagcc ctgaccacac actcgacgca cttggcctgc gctgcccgga accagtaatg
    11041 atggtgcgca aaacagtgcg caatatgcag ccaggcgaaa cgttgctgat tatcgccgac
    11101 gatccggcca ctacccgcga tattcctggg ttttgtacct ttatggaaca cgaactggtt
    11161 gctaaagaga cggatgaact gccttatcgt tatttgattc gcaaaggcgg ttgatgggga
    11221 aagattggct tcgatgccgc cttttcccct caccctaacc ctctccccag aggagcgagg
    11281 ggaccgatcg cgctcaatgt tgcgatcggt ttgccttatc tcctgcgcaa caatcttaac
    11341 gcattcgctg tcaccagcac cgtcgcaccc gtatctgcca gcaccgccag ccatagcccg
    11401 gtcattccta acagtgtggt gacgaggaag atccctttca gccccagcgc aatagtgatg
    11461 ttctggcgga tattggcgtg agtggcgcgt gccagttcaa tcatttgcac caggccgcgc
    11521 aggtggttat gggttaatgc tgcatcggcg gtttccagcg ccacgtctgt gccactgccc
    11581 attgcaatcc cgatggcggc ggctttcatc gctggcgcgt cgttaatacc gtcaccgacc
    11641 atcgccagcg gcgcatgttg attcagctcg gtcaccgctt tgactttatc ttccggcaac
    11701 aggcccgctt taaactctag ccccagttcc ccggcgattg ccgccgctgc gcgtggatta
    11761 tcgccggtga ggatcacccc tttgacgccc agcgcgttca gttcactgat ggcagttgca
    11821 gcatcggcgc gcagggtatc ctgtaacgca atgacaccaa gcacgtcatc gttacgtact
    11881 accagcacta ccgtttgccc ggcgctttcc agttcgttaa ttaaaccagc aaatgcatca
    11941 gcgggatgtt tcccggcagc gcatatcaac acgcgctcac cgttaacctg cgcttcaatg
    12001 ccagacccaa ccagcgcccg ctgtgattgg gcggcgggaa tggcgagttc agcaacctgt
    12061 gcttctcgca cgatggcttg cgccagtgga tgcgtcgcgc cttgctcgac cgccgctgcc
    12121 agtgtcagca gttcggattc actaataccc gttgccggat gaatcgcggt aacgcgcggt
    12181 ttaccgacgg tcagcgtacc ggttttatca aacgccacct gggtaacacg acccagttgt
    12241 tccagcgccg ctccgccttt aattaacgcc ccacgacgcg ctgctgctgc cagcccggag
    12301 gtaatcgccg caggcgttga gataactaac gcacacgggc agccaatcag cagcagcgtc
    12361 agccctttat aaatccactc ttgccagctg gcggcaaaca gcagtggtgg caccagcgtt
    12421 accagcagag cgacggccat aatcgctggc gtgtaaatac ggctgaaacg gtcgataaac
    12481 cgctcaatgg gagcgcgacg ctcttcggct tcttcgatca gtttcagaat ccggtcaatg
    12541 gcgctggcac ccggttctga cagcacttcc agcattacca gacggtctac gctggtggcg
    12601 cctgcaggaa ctttatcgcc cgtcgcgcgc tccacaggaa tggattctcc ggtcagggcg
    12661 ctttcatcaa aactggcaaa cggtgagagc agtttaccgt cggcaggcaa acgcccacct
    12721 gccgcaactt caatcacatc gccaggccgc aggctgttta ttgccacctc ttcacgctca
    12781 ccgttacgca gacgcgtggc ggtttctggc ttcagcgcca ttaacgcgct gaccccttga
    12841 cgcgcgcgac tggcggccca gccctccagt cgttcgccaa tcaaaaacag cagcaacacc
    12901 atcgcagctt cagccgttgc gccaataaac agcgcaccaa tagcggctac gctcattaac
    12961 gtttcaatgg cgaagtagct gccggatttg atcagccgta atgcctgacg agcaatcggg
    13021 tacagcccaa ccagcgtggt cgcgataaac gccagttgcc cgaacggatg attgaactgc
    13081 tccagacccc agctgattgc catcatcacg attagcgtaa tcagcggcag attctctttc
    13141 aggcgtgatg cttgtggttc gtcggcggac tgttcatcgc gcagggaata gcctgctttt
    13201 tgcaccgcag attcaacctg ggcacggatg tcattgtcgg catcgaccac cagtttttcg
    13261 gtggcgaaca acacctgcac ctgattcacg cctgcaagct ggcgcacggc attttctacc
    13321 ttgcgcgcac aggcggcgca gtccatgccg ctgactttcc agctatagcg ggtgccggag
    13381 acgttttcag agagagttgg cgtgctggaa catgcgccgt cgcagcaaca gtcgttggcg
    13441 ttctgtaccg tggttagcgg tttgaacgca gcaaattgag gggctttctt gccgtgattg
    13501 tcaggagtcg acatggcatc ctccggttaa gttttttctc attaaccgaa ggatacactc
    13561 tggagtcgac tccagagtca agttttatca gagatacagc gagcggacga tgaggaaatg
    13621 accggcaaag tagcaggccg cagcaatcgc gttatctgcc cggaagcggc ggcgatagtg
    13681 gctacccagc cagacaaagt tactgataaa cagcaacgaa gcgccgacaa acgcagagag
    13741 cgccggagct gtcggacgga agaaccacag ttcacctgcc agccacacca tcaccagcgt
    13801 catgccgata aacgtgcaga taggccaacg atactcttcc agacgcgtcc agataatcgc
    13861 cagtaacagc gcacccagaa ccagcaacac cagtggcaga ggccagaaga aagagagcgt
    13921 catctgactg gcaaaataga tggtgtacag caggtgcgag aggaaaaacg cgccgatggc
    13981 gtacatcaga cgttgacgtg gcaacagggt tagcgcatcg cccagcagtg aggcgcacag
    14041 ccctgccagc accagatagc taatggcgtc gaacatcggc gcttgccagg ccagtaacag
    14101 caggagaaga agggttaacg gtttaaacac ccagcgttgc caggttggcc cacgatacga
    14161 tgcatccaca gatagccatg cggaaagaca gacagcgata aacgaccaaa gcatcttagt
    14221 tccttgaatt cgttattttt tcgtaagcgg aatgcctacg gtcttgcttc cagtttagtg
    14281 gcgagggtgg acggatgaca acaccataac tggcagggta tgcttatttc cgcattttcc
    14341 gatgagggat aaagatgagc aaaccacctc ttttctttat tgttatcatt ggcttaattg
    14401 tcgtcgccgc atcgtttcgt tttatgcaac agcgacggga aaaagctgat aatgatatgg
    14461 ctccgctcca gcaaaagctg gtggtggtga gcaacaagcg ggaaaaaccg attaacgatc
    14521 gccgttcgcg ccagcaggaa gtgactccgg caggcaccag tatgcgctac gaggcaagct
    14581 tcaaaccgca aagcggagga atggagcaga cgtttcgcct cgacgctcag cagtaccatg
    14641 ccctgacagt gggcgatata ggtacgctga gctataaagg aacgcgcttt gttagctttg
    14701 taggcgaaca ataaggttat tttttaactt taaatttctt ctgccagacc agcagttcaa
    14761 acacgccgaa aaggaaaatc cgcacctttt cggcggtggt catctgcggg ccatctttag
    14821 gtaaagtgga tttcaatagc gccagctgca taccatgcat cagcacggta aaaatcagcg
    14881 ccacgttaac gaaaatattc agcgggcgcg ggaagggatg caccagattg aggattaaaa
    14941 atccccaaac gcagagcatt aacaaacgac caatattaat cagcatcact ttctccttgt
    15001 gcttcgcgtt gatacagccg ataagccacc tgacccgcca ctttttcccg atgtaatgac
    15061 cagtttgccg gaacagtggg cagaccgttt tcgacttcgc tttcgacata aatcagggct
    15121 tcgtcagcca gccagccgtt atcttccagt aaatttatcg tctcttctaa caagccacgg
    15181 cggaacggtg gatcgacaaa cacgatatta tgcggtgtgc ctttttgcgc caggaatgac
    15241 attgcgttgc tgttcaccac gcgtgcattg cctgctttta gtgtcgccag attcttaatt
    15301 aactgctgag aaaccgcgcg atccatctca atcaacgtgg cccccgcagc atagcgcgac
    15361 aacgcttcca gccccagcgc gccgctcccg gcgaagcaat ccagacattg ggcgtcaaca
    15421 atgaccggag ccagccagtt aaacaacgtt tcgcgtacgc ggtcggtggt ggggcgcaga
    15481 cctgggctat caggaaccgg gagtttacgg cctcgccact gcccgccaat aatgcggatt
    15541 tggccgctgc cggaatgatt cggttttttc atgataaatt gctcaatccg ccagataaca
    15601 catacttgca ggcggtgatg tgaattaagt taagtgatag actatttcat cattttttta
    15661 gctgctatgt acatagcgtt aacgctgtgc catgaagcaa cagcgaggag tgtagtcgca
    15721 aatggcgaaa gaaaaaaaac gtggcttttt ttcctggctg ggctttggtc aaaaagagca
    15781 gaccccggaa aaagagacag aagttcagaa tgaacaaccg gttgtagaag aaatcgttca
    15841 ggcgcaagag cctgcgaagg cttcggaaca agccgttgaa gagcagccgc aggcgcatac
    15901 tgaggccgaa gcggaaactt ttgctgccga cgttgtggaa gtcactgaac aggttgttga
    15961 aagtgaaaaa gcgcaacctg aagcggaagt tgttgcacag ccggaactgg tcgtagaaga
    16021 aacgccggag ccagtggcta tcgagcgtga agagctgccg ttgccggaag acgtcaacgc
    16081 cgaagaggtt tcgccagaag agtggcaggc cgaagcggaa accgtagaga ttgtcgaagc
    16141 ggcggaagaa gaagcggcta aagaagaaat caccgacgaa gagctggaag cacaggcgct
    16201 ggctgccgaa gcagcagaag aggcggtgat ggtggttcct ccggcagaag aagagcagcc
    16261 ggtagaagaa atcgctcagg agcaggaaaa accgaccaaa gaaggtttct tcgcgcgcct
    16321 gaaacgcagc ctgttaaaaa ctaaagaaaa tctcggttcc ggatttatca gcctgttccg
    16381 cggtaaaaaa atcgacgatg atctgtttga agagctggaa gaacagctgt tgatcgccga
    16441 tgtgggcgtg gaaaccacac gtaaaataat caccaatctg acggaaggcg catcccgcaa
    16501 gcagcttcgt gatgccgagg cgctttatgg cctgctgaaa gaagagatgg gcgagattct
    16561 ggcgaaagtc gatgagccgc tgaatgttga aggtaaaacg ccgttcgtga tcctgatggt
    16621 gggcgtcaac ggtgtgggta aaaccacgac gattggtaag ctggcacgtc agtttgagca
    16681 gcagggtaaa tcggtgatgc tggcggcggg cgatactttc cgtgcggcag cggttgaaca
    16741 gcttcaggtc tggggtcagc gcaacaatat tccggtgatt gcccagcata ccggggcgga
    16801 ttccgcctct gttatcttcg acgccattca ggcagctaaa gcgcgtaata tcgacgtcct
    16861 gattgccgat accgccggac gcctgcagaa caaatcgcac ctgatggaag agttgaagaa
    16921 aatcgtccgc gtgatgaaga aactcgacgt tgaagcgccg catgaagtta tgctgactat
    16981 tgatgccagc accgggcaga acgcggtaag ccaggccaaa ctgttccatg aagccgttgg
    17041 cttaaccggc atcacgctaa cgaaactgga cggcacggcg aaaggcggag taattttctc
    17101 ggtggctgac cagtttggta tccctatccg ctacattggt gtcggcgaac gtattgagga
    17161 tttgcgtccg tttaaggcgg acgactttat agaggcactt tttgcccgag aggattaaca
    17221 atgattcgct ttgaacatgt cagcaaggct tatctcggtg ggagacaggc gctgcagggc
    17281 gttacgttcc atatgcagcc gggtgagatg gcgtttctga ccggtcattc cggcgcaggg
    17341 aaaagtaccc tcctgaagct gatctgtggg attgagcggc ccagcgccgg gaaaatctgg
    17401 tttagcggcc atgacatcac gcgtctgaaa aaccgtgaag ttccgtttct gcgccgccag
    17461 attggtatga ttttccagga tcaccatcta ctgatggacc gtactgtcta cgataacgtg
    17521 gcgatcccgc tgattatcgc cggtgccagc ggtgacgata ttcgtcgccg ggtgtcggcg
    17581 gcactggata aagtcgggct actggacaaa gcgaagaact ttcctattca gctttccggc
    17641 ggtgaacaac agcgtgttgg cattgcccgc gcggtggtga ataagcccgc ggtactgctg
    17701 gcggacgaac cgactggtaa cctggacgac gcgttgtcgg aaggcatttt acgtctgttt
    17761 gaagagttta accgcgttgg ggtaaccgta ttgatggcaa cgcacgacat caacctgatt
    17821 tcgcggcgtt cctatcgcat gctcaccctg agcgatggtc acttgcatgg aggcgtgggc
    17881 catgaataag cgcgatgcaa tcaatcatat tcggcagttt ggcgggcgtc tggatcgctt
    17941 ccgtaaatcg gtcggcggct caggcgacgg cggtcgtaac gcaccgaaac gcgcgaaatc
    18001 ctcgccaaaa ccggtaaatc gcaaaaccaa cgttttcaac gaacaggtgc gctatgcctt
    18061 ccacggcgca ttgcaggatc tgaaaagcaa accgttcgcc acgtttttaa cggtgatggt
    18121 tatcgccatc tccctgacgc tgcccagcgt ctgttatatg gtgtacaaaa acgttaacca
    18181 ggcggcgacg caatattatc cgtcaccgca aatcactgtt tatctgcaaa aaacgctgga
    18241 cgatgacgcc gctgcgggtg tggtggcaca gttacaggcc gagcaaggcg tagagaaagt
    18301 gaattatctt tctcgtgaag acgcactggg cgagttccgt aactggtctg gttttggtgg
    18361 tgcgctggat atgctggaag aaaacccgct tccggcagtg gcggtggtga tcccgaaact
    18421 cgattttcag gggacggaat cactgaatac gctgcgtgat cgtatcaccc agatcaacgg
    18481 cattgacgaa gtgcggatgg atgacagctg gtttgcccgt ctggcggcgt taaccgggct
    18541 ggtcgggcgc gtttccgcga tgatcggcgt gttgatggtg gcagcagtgt tcctcgtcat
    18601 cggcaacagt gtgcgtctga gcatctttgc tcgccgtgac tccattaacg tgcagaaact
    18661 gattggtgcg acggatggtt tcatcctgcg cccgttcctg tacggaggcg cattgctggg
    18721 attttctggc gcattgctgt cattaatttt gtcagaaatt ctggtactgc gtttgtcatc
    18781 ggcggttgcg gaagtggcac aggttttcgg aacaaagttt gatatcaatg gcttatcgtt
    18841 cgatgaatgc ctgctgttgc tgctggtatg ctcgatgatt ggctgggtgg cagcgtggct
    18901 tgccacggta caacatttac gccactttac gccggaataa taaaagcgtg ttatactctt
    18961 tccctgcaat gggttccgta gcagggaaag agaccccgtt gtctcttccc ggtatttcat
    19021 ctctatgtca cattttgtgc gtaatttatt cacaagcttg cattgaactt gtggataaaa
    19081 tcacggtctg ataaaacagt gaatgataac ctcgttgctc ttaagctctg gcacagttgt
    19141 tgctaccact gaagcgccag aagatatcga ttgagaggat ttgaatgact gacaaaatgc
    19201 aaagtttagc tttagcccca gttggcaacc tggattccta catccgggca gctaacgcgt
    19261 ggccgatgtt gtcggctgac gaggagcggg cgctggctga aaagctgcat taccatggcg
    19321 atctggaagc agctaaaacg ctgatcctgt ctcacctgcg gtttgttgtt catattgctc
    19381 gtaattatgc gggctatggc ctgccacagg cggatttgat tcaggaaggt aacatcggcc
    19441 tgatgaaagc agtgcgccgt tttaacccgg aagtgggtgt gcgcctggtc tccttcgccg
    19501 tccactggat caaagcagag atccacgaat acgttctgcg taactggcgt atcgtcaaag
    19561 ttgcgaccac caaagcgcag cgcaaactgt tcttcaacct gcgtaaaacc aagcagcgtc
    19621 tgggctggtt taatcaggat gaagttgaaa tggtggcccg tgaactgggc gtaaccagca
    19681 aagacgtacg tgagatggag tcgcgtatgg cggcacagga catgaccttt gacctgtctt
    19741 ccgatgacga ttccgacagc cagccgatgg ctccggtgct ctatctgcag gataaatcat
    19801 ctaactttgc cgacggcatt gaagatgata attgggaaga gcaagcggca aaccgtctga
    19861 ccgacgcgat gcaaggtctg gacgagcgta gccaggatat catccgcgcg cgctggctgg
    19921 atgaagacaa caagtccacg ttgcaggaac tggctgaccg ttacggtgtt tccgctgagc
    19981 gtgtgcgcca gctggaaaag aatgcgatga aaaaacttcg cgctgccatt gaagcgtaat
    20041 ttccgctact cagcagagaa ccctggatga gaatccgggg tttttgtttt ttgggcctct
    20101 gcaataatca gtttccccct ccggcaaaac gccaatcccc acgcagattg ttaataaact
    20161 gtcaaaatag ctattccaat atcataaaaa tcgggtatgt tttagcagag tatgctgcta
    20221 aagcacgggt agtcatgcat aaaacgaaat aaagtgctga acaacaacat cacaacacac
    20281 gtaataacca gaagaatggg gattctcagg atgaacataa agggtaaagc gttactggca
    20341 ggatgtatcg cgctggcatt cagcaatatg gctctggcag aagatattaa agtcgccgtc
    20401 gtaggcgcaa tgtccggtcc ggtggcgcag tacggtgacc aggagtttac tggcgcagag
    20461 caggcggttg cggatatcaa tgctaaaggt ggtatcaaag gcaacaaact gcaaatcgta
    20521 aaatatgacg atgcctgtga cccgaaacag gcggttgcgg tggcgaacaa agtcgttaac
    20581 gacggcatta aatacgtgat tggtcacctc tgctcttcat caacgcagcc agcgtctgac
    20641 atctacgaag acgaaggcat tttaatgatc accccagcgg caaccgcacc agagctgacc
    20701 gcccgtggct atcagctgat cctgcgcacc accggcctgg actccgacca ggggccgacg
    20761 gcggcgaaat atattcttga gaaagtgaaa ccgcagcgta ttgctatcgt tcacgacaaa
    20821 cagcaatacg gcgaaggtct ggcgcgagcg gtgcaggacg gcctgaagaa aggcaatgct
    20881 aacgtggtgt tctttgatgg tatcaccgcc ggggaaaaag atttctcaac gctggtggcg
    20941 cgtctgaaga aagagaatat cgacttcgtt tactacggcg gttatcaccc ggaaatgggg
    21001 caaatcctgc gtcaggcacg cgcggcaggg ctgaaaactc agtttatggg gccggaaggc
    21061 gtggctaacg tctcgctgtc taacattgcg ggtgaatcag cggaaggact gctggtgacc
    21121 aagccgaaga actacgatca ggttccggcg aacaaaccca ttgttgacgc gatcaaagcg
    21181 aagaaacagg acccaagcgg cgcgttcgtc tggaccacct acgccgcgct gcaatctttg
    21241 caggtgggcc tgaatcagtc tgacgatccg gctgaaatag ccaaatacct gaaagcgaac
    21301 tccgtggata cggtgatggg gccgctgacc tgggatgaga aaggtgatct gaaaggcttt
    21361 gagttcggcg tatttgactg gcacgccaac ggcacggcga ctgatgcgaa gtaatcatta
    21421 atcggcaact ttgggttgcc gccaaattgc taatatcgag tacgttgctt catgccggat
    21481 gcggcgtaaa cgccttatcc ggcctacaag attcaaagaa atcagtaaat tgcagcacac
    21541 attgtaggcc tgataagcgt agcgcatcag gcaatatcac ttgtgaaatc aaacttgacg
    21601 attaacgctt ctcccagccg ccctgttgtg ccgtaaatcc cagtgcctgc ataaacgctg
    21661 tcatcacgcc gcgatcttcc acgcctgcat ccgccatcca ccagcatgaa acgccaggat
    21721 tgttacgcaa aacctcttcc agcagatatt gccccacacc gcgacggcgg gtgacttccc
    21781 tcacacgcag ggaatccagt gccccctcgg tgccgcttaa ggttacccgc acggccgcga
    21841 gaaggcgctc gttaaaacgc gcggcgtaga tacggtggtt atcgtcaacc tgtaaagagg
    21901 aaggggaata ctccggccag atcttttgca ggtcaatccg gtcctggtcg ctaaagtttt
    21961 ctaatcgaat gatggtcagc ttcatgggga acccgtgtaa atcacaaaag tggaaccagt
    22021 gtagcgaaat aatttaatcg gaggctttct cttttttaat tcttttggca ggtgattaat
    22081 tttttaacag caataattac aaagttaaaa cattagagaa t
//
LOCUS       DAZFLD010000067        22081 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.067, whole genome shotgun sequence.
ACCESSION   DAZFLD010000067 DAZFLD010000000
VERSION     DAZFLD010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22081)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22081)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22081
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.067"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            133..1830
                     /gene="ypdA"
                     /locus_tag="ACHKBL_002163"
     CDS             133..1830
                     /gene="ypdA"
                     /locus_tag="ACHKBL_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     YpdA"
                     /protein_id="HFO5819039.1"
                     /translation="MHEIFNMLLAVFDRAALMLICLFFLIRIRLFRELLHKSAHSPKE
                     LLAVTAIFSLFALFSTWSGVPVEGSLVNVRIIAVMSGGILFGPWVGIITGVIAGIHRY
                     LIDIGGVTAIPCFITSILAGCISGWINLKIPKAQRWRVGILGGMLCETLTMILVIVWA
                     PTTALGIDIVSKIGIPMILGSVCIGFIVLLVQSVEGEKEASAARQAKLALDIANKTLP
                     LFRHVNSESLRKVCEIIRDDIHADAVAITNTDHVLAYVGVGEHNYQNGDDFISPTTRQ
                     AMNYGKIIIKNNDEAHRTPEIHSMLVIPLWEKGVVTGTLKIYYCHAHQITSSLQEMAV
                     GLSQIISTQLEVSRAEQLREMANKAELRALQSKINPHFLFNALNAISSSIRLNPDTAR
                     QLIFNLSRYLRYNIELKDDEQIDIKKELYQIKDYIAIEQARFGDKLTVIYDIDEEVNC
                     CIPSLLIQPLVENAIVHGIQPCKGKGVVTISVAECGNRVRIAVRDTGHGIDPKVIERV
                     EANEMPGNKIGLLNVHHRVKLLYGEGLHIRRLEPGTEIAFYIPNQRTPVASQATLLL"
     gene            1845..2579
                     /gene="ypdB"
                     /locus_tag="ACHKBL_002164"
     CDS             1845..2579
                     /gene="ypdB"
                     /locus_tag="ACHKBL_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator YpdB"
                     /protein_id="HFO5819040.1"
                     /translation="MKVIIVEDEFLAQQELSWLIKEHSQMEIVGTFDDGLDVLKFLQH
                     NRVDAIFLDINIPSLDGVLLAQNISQFAHKPFIVFITAWKEHAVEAFELEAFDYILKP
                     YQESRITGMLQKLEAAWQQQQTSSTPAATVTRENDTINLVKDERIIVTPINDIYYAEA
                     HEKMTFVYTRRESYVMPMNITEFCSKLPPSHFFRCHRSFCVNLNKIREIEPWFNNTYI
                     LRLKDLDFEVPVSRSKVKEFRQLMHL"
     gene            2592..3449
                     /gene="ypdC"
                     /locus_tag="ACHKBL_002165"
     CDS             2592..3449
                     /gene="ypdC"
                     /locus_tag="ACHKBL_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO5819041.1"
                     /translation="MKAPGLPADQQFFADLFSGLVLNPQLLGRVWFASQPASLPVGSL
                     CIDFPRLDIVLRGEYGNLLEAKQQRMVEGEMLFIPARAANLPINNKPVMLLSLVFAPT
                     WLGLSFYDSRTTSLLHPVRQIQLPSLQRGEGEAMLTALTHLSRSPLEQNIIQPLVLSL
                     LHLCRNVVNMPPGNSQPRGDFLYHSICNWVQDNYAQPLTRESVAQFFNITPNHLSKLF
                     AQHGTMRFIEYVRWVRMAKARMILQKYHLSIHEVAQRCGFPDSDYFCRVFRRQFGLTP
                     GEYSARFQG"
     gene            complement(3452..5947)
                     /gene="ptsP"
                     /locus_tag="ACHKBL_002166"
     CDS             complement(3452..5947)
                     /gene="ptsP"
                     /locus_tag="ACHKBL_002166"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFO5819042.1"
                     /translation="MLTIQFLCPLPNGLHARPAWELKEQCSQWQSEITFINHRQNAKA
                     DAKSSLALIGTGTLFNDGCSLNISGSDEEQARRVLEEYIQVRFIDSDSVQPTQAELTA
                     HPLPRSLSRLNPDLLYGNVLASGVGVGTLTLLQSDSLDSYRAIPASAQDSTRLEHSLA
                     TLAEQLNQQLRERDGESKTILSAHLSLIQDDEFAGNIRHLMAEQHQGLGAAIISNMEQ
                     VCAKLSASASDYLRERVSDIRDISEQLLHITWPELKPRNNLVLEKPTILVAEDLTPSQ
                     FLSLNLKNLAGMILEKTGRTSHTLILARASAIPVLSGLPLDAIARYAGQPAVLDAQCG
                     VLAINPNDAVSGYYQVAQMLADKRQQQQAQAAAQLAYSRDNKRIDIAANIGTALEAPG
                     AFANGAEGVGLFRTEMLYMDRDSAPDEQEQFEAYQQVLLAAGDKPIIFRTMDIGGDKS
                     IPYLNIPQEENPFLGYRAVRIYPEFAGLFRTQLRAILRAASFGNAQLMIPMVHSLDQI
                     LWVKGEIQKAIVELKRDGLRHAETITLGIMVEVPSVCYIIDHFCDEVDFFSIGSNDMT
                     QYLYAVDRNNPRVSPLYNPITPSFLRMLQQIVTTAHQRGKWVGICGELGGESRYLPLL
                     LGLGLDELSMSSPRIPAVKSQLRQLDSEACRELARQACECRSAQEIEALLTAFTPEED
                     VRPLLALENIFVDQAFSNKEQAIQFLCGNLGVNGRTEHPFELEEDVWQREEIVTTGVG
                     FGVAIPHTKSQWIRHSSISIARLVKPVDWQSEMGEVELVIMLTLGANEGMNHVKVFSQ
                     LARKLVNKNFRQSLFAAQDAQSILTLLETELTF"
     gene            complement(5972..7009)
                     /gene="ypdE"
                     /locus_tag="ACHKBL_002167"
     CDS             complement(5972..7009)
                     /gene="ypdE"
                     /locus_tag="ACHKBL_002167"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFO5819043.1"
                     /translation="MDLSLLKALSEADAIASSEQEVRQILLEEADRLQKEVRFDGLGS
                     VLIRLNESTGPKVMICAHMDEVGFMVRSISREGAIDVLPVGNVRMAARQLQSVRITTR
                     EECKIPGLLDGDRQGNDVSTIRVDIGARSYDEVMQAGIRPGDRVTFDTTFQVLPHQRV
                     MGKAFDDRLGCYLLVTLLRELHDAELPAEVWLVASSSEEVGLRGGQTATRAVSPDVAI
                     VLDTACWAKNFDYGAANHRQIGNGPMLVLSDKSLIAPPKLTAWIETVAAEIGVPLQAD
                     MFSNGGTDGGAVHLTGTGVPTVVMGPATRHGHCAASIADCRDILQMQQLLSALIQRLT
                     RETVVQLTDFR"
     gene            complement(7009..8094)
                     /gene="ypdF"
                     /locus_tag="ACHKBL_002168"
     CDS             complement(7009..8094)
                     /gene="ypdF"
                     /locus_tag="ACHKBL_002168"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFO5819044.1"
                     /translation="MTLLASLRDWLKAQQLDAVLLSSRQNKQPHLGISTGSGYVVICR
                     ESAHILVDSRYYADVEARTQGYQLHLLDAMHTLATIVRQIFADEQLQTLGFEGQQVSW
                     ETAHRWQSELNAKLVSATPDVLRQLKTPEEVEKIRLACGIADRGAEHIRRFIQAGMSE
                     REIAAELEWFMRQQGAEKASFDTIVASGWRGALPHGKASDKIVAAGEFVTLDFGALYQ
                     GYCSDMTRTLLVNGEGVSAESHPLFNVYQIVLQAQLAAISAIRPGVRCQQVDDAARRV
                     ITEAGYGDYFGHNTGHAIGIEVHEDPRFSPRDTTTLQPGMLLTVEPGIYLPGQGGVRI
                     EDVVLVTPQGVEVLYAMPKTVLLTGEA"
     gene            complement(8109..9356)
                     /gene="fryC"
                     /locus_tag="ACHKBL_002169"
     CDS             complement(8109..9356)
                     /gene="fryC"
                     /locus_tag="ACHKBL_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFO5819045.1"
                     /translation="MAIKKRSATVVPGASGAAAAVKNPQASKTSFWGELPQHVMSGIS
                     RMVPTLIMGGVILAFSQLIAYSWLKIPADIGIMDALNSGKFSGFDLSLLKFAWLSQSF
                     GGVLFGFAIPMFAAFVANSIGGKLAFPAGFIGGLMSTQPTQLLNFDPSTMQWATSSPV
                     PSTFIGALIISIVAGYLVKWMNQKIQLPDFLLAFKTTFLLPILSAIFVMLAMYYVITP
                     FGGWINGGIRTVLTAAGEKGALMYAMGIAAATAIDLGGPINKAAGFVAFSFTTDHVLP
                     VTARSIAIVIPPIGLGLATIIDRRLTGKRLFSAQLYPQGKTAMFLAFMGISEGAIPFA
                     LESPITAIPSYMVGAIVGSTAAVWLGAVQWFPESAIWAWPLVTNLGVYMAGIALGAVI
                     TALMVVFLRLMMFRKGKLLIDSL"
     gene            complement(9378..9704)
                     /gene="fryB"
                     /locus_tag="ACHKBL_002170"
     CDS             complement(9378..9704)
                     /gene="fryB"
                     /locus_tag="ACHKBL_002170"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_function: GO:0090563 - protein-phosphocysteine-sugar
                     phosphotransferase activity [Evidence IEA];
                     GO_function: GO:0090582 -
                     protein-phosphocysteine-D-fructose-phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HFO5819046.1"
                     /translation="MSKKLIALCACPMGLAHTFMAAQALEEAAVEAGYEVKIETQGAD
                     GIQNRLTAQDIAEATIIIHSVAVTPEDNERFESRDVYEITLQDAIKNAAGIIKEIEEM
                     IASEQQ"
     gene            complement(9923..10888)
                     /gene="glk"
                     /locus_tag="ACHKBL_002171"
     CDS             complement(9923..10888)
                     /gene="glk"
                     /locus_tag="ACHKBL_002171"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005536 - D-glucose binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HFO5819047.1"
                     /translation="MTKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAV
                     IRVYLEEHKIEVQDGCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFT
                     AVSMAIPMLKKEHLIQFGGAEPVEGKPIAVYGAGTGLGVAHLVHVDKRWVSLPGEGGH
                     VDFAPNSEEEGIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDI
                     TERALADSCTDCRRALSLFCVIMGRFGGNLALNLGTFGGVFIAGGIVPRFLEFFKASG
                     FRAAFEDKGRFKEYVHDIPVYLIVHDNPGLLGSGAHLRQTLGHIL"
     gene            11092..12348
                     /gene="yfeO"
                     /locus_tag="ACHKBL_002172"
     CDS             11092..12348
                     /gene="yfeO"
                     /locus_tag="ACHKBL_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel protein"
                     /protein_id="HFO5819048.1"
                     /translation="MLHPRARTMLLLSLPAVAIGIASSLILIVVMKIASVLQNLLWQR
                     LPGTLGIAQDSPLWIIGVLTLTGIAVGLVIRFSQGHAGPDPACEPLIGAPVPPSALPG
                     LIVALILGLAGGVSLGPEHPIMTVNIALAVAIGARLLPRVNRMEWTILASAGTIGALF
                     GTPVAAALIFSQTLNGSSEVPLWDRLFAPLMAAAAGALTTGLFFHPHFSLPIAHYGQM
                     QMTDILSGAIVAAIAIAAGMVAVWCLPRLHAMMHQMKNPVLVLGIGGFILGILGVMGG
                     PVSLFKGLDEMQQMVANQAFSTSDYFLLAVIKLAALVVAAASGFRGGRIFPAVFVGVA
                     LGLMLHEHVPAVPAAITVSCAILGIVLVVTRDGWLSLFMAAVVVPNTTLLPLLCIVML
                     PAWLLLAGKPMMMVNRPKQQPPHDNV"
     gene            12463..12789
                     /gene="ypeC"
                     /locus_tag="ACHKBL_002173"
     CDS             12463..12789
                     /gene="ypeC"
                     /locus_tag="ACHKBL_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein YpeC"
                     /protein_id="HFO5819049.1"
                     /translation="MFRSLFLAAALMAFTPLAANAGEITLLPSIKLQIGDRDHYGNYW
                     DGGHWRDRDYWHRNYEWRKNRWWRHDNGYHRGWDKRKAYERGYREGWRDRDDHRGKGR
                     GHGHRH"
     gene            complement(12930..14168)
                     /gene="mntH"
                     /locus_tag="ACHKBL_002174"
     CDS             complement(12930..14168)
                     /gene="mntH"
                     /locus_tag="ACHKBL_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HFO5819050.1"
                     /translation="MTNYRVESSSGRAARKMRLALMGPAFIAAIGYIDPGNFATNIQA
                     GASFGYQLLWVVVWANLMAMLIQILSAKLGIATGKNLAEQIRDHYPRPVVWFYWVQAE
                     IIAMATDLAEFIGAAIGFKLILGVSLLQGAVLTGIATFLILMLQRRGQKPLEKVIGGL
                     LLFVAAAYIVELIFSQPNLAQLGKGMVIPSLPTSEAVFLAAGVLGATIMPHVIYLHSS
                     LTQHLHGGSRQQRYSATKWDVAIAMTIAGFVNLAMMATAAAAFHFSGHTGVADLDEAY
                     LTLQPLLSHAAATVFGLSLVAAGLSSTVVGTLAGQVVMQGFIRFHIPLWVRRTVTMLP
                     SFIVILMGLDPTRILVMSQVLLSFGIALALVPLLIFTSDSKLMGDLVNSKRVKQTGWV
                     IVVLVVALNIWLLVGTALGL"
     gene            14504..15706
                     /gene="nupC"
                     /locus_tag="ACHKBL_002175"
     CDS             14504..15706
                     /gene="nupC"
                     /locus_tag="ACHKBL_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupC"
                     /protein_id="HFO5819051.1"
                     /translation="MDRVLHFVLALAVVAILALLVSSDRKKIRIRYVIQLLVIEVLLA
                     WFFLNSDVGLGFVKGFSEMFEKLLGFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFIS
                     ALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
                     ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVD
                     ASEENIQMSNLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTG
                     WFGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSNEFVAMMDLQKIAST
                     LSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLS
                     ASIAALVL"
     gene            complement(15755..17944)
                     /gene="pdeA"
                     /locus_tag="ACHKBL_002176"
     CDS             complement(15755..17944)
                     /gene="pdeA"
                     /locus_tag="ACHKBL_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
                     /protein_id="HFO5819052.1"
                     /translation="MFVEHNLIKNIKIFTLAFTLTVVLIQLSRFISPLAIIHSSYIFL
                     AWMPLCVMLSILFIFGWRGVVPVLCGMFCTNLWNFHLSFLQTAVMLGSQTFVVLCACA
                     ILRWQLGTRWRYGLTSRYVWQRLFWLGLVAPIGIKCSMYLVGNFFDFPLKISTFFGDA
                     DAIFTVVDLLSLFTAVLIYNMLFYYLARMIVSPHFAQILWRRDIAPSLSKEKRAFTLS
                     WLAALSVLLLLMCTPYENDFIAGYLVPVFFIIFTLGVGKLRYPFLNLTWAVSTLCLLN
                     YNQNFLQGVLTEYSLAFILAVLISFSVCLLYMVRIYHRSEWLNRRWHLQALTDPLTLL
                     PNFRALEQAPEQEAGKSFCCLRIDNLEFMSRHYGLMMRVHCIRSIYRTLLPLMQENEK
                     LYQLPGSELLLVLSGPETEGRLQHMVNILNSRQIHWNNTGLDMGYGAAWGRFDGNQET
                     LQPLLGQLSWLAEQSCAHHHVLALDSREEMVSGQTTKQVLLLNTIRTALDQGDLLLYA
                     QPIRNKEGEGYDEILARLKYDGGIMTPDKFLPLIAQFNLSARFDLQVLESLLKWLATH
                     PCDKKGPRFSVNLMPLTLLQKNIAGRIIRLFKRYHISPQAVILEITEEQAFSNAESSM
                     YNIEQLHKFGFRIAIDDFGTGYANYERLKRLQADIIKIDGVFVKDIVTNTLDAMIVRS
                     ITDLAKAKSLSVVAEFVETQQQQALLHKLGVQYLQGYLIGRPQPLAD"
     gene            complement(18152..18227)
                     /locus_tag="ACHKBL_002177"
     tRNA            complement(18152..18227)
                     /locus_tag="ACHKBL_002177"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18192..18194),aa:Ala,seq:ggc)
     gene            18448..18807
                     /gene="yfeC"
                     /locus_tag="ACHKBL_002178"
     CDS             18448..18807
                     /gene="yfeC"
                     /locus_tag="ACHKBL_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeC-like transcriptional regulator"
                     /protein_id="HFO5819053.1"
                     /translation="MFKERMTPDELARLTGYSRQTINKWVRKEGWTTSPKPGVQGGKA
                     RLVHVNEQVREYIRNAERPEGQGEAPALSGDAPLEVLLVTLAKEMTPVEQKQFTSLLL
                     REGIIGLLQRLGIRDSK"
     gene            18809..19201
                     /gene="yfeD"
                     /locus_tag="ACHKBL_002179"
     CDS             18809..19201
                     /gene="yfeD"
                     /locus_tag="ACHKBL_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003834896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeC-like transcriptional regulator"
                     /protein_id="HFO5819054.1"
                     /translation="MKRLRNKMTTEELAECLGVAKQTVNRWIREKGWKTEKFPGVKGG
                     RARLILVDTQVCEFIQNTPAFHNTPMLLEAEEPLAEYAPGIRTPAYRQIISAIDNMTH
                     SEQEKVAQFLSREGIRNFLARLDIDESE"
     gene            complement(19252..20667)
                     /gene="gltX"
                     /locus_tag="ACHKBL_002180"
     CDS             complement(19252..20667)
                     /gene="gltX"
                     /locus_tag="ACHKBL_002180"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HFO5819055.1"
                     /translation="MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDT
                     DLERSTPEAIEAIMDGMNWLSLEWDEGPYYQTKRFDRYNAVIDQMLEEGTAYKCYCSK
                     ERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
                     EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALK
                     APVPVYAHVSMINGDDGKKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEI
                     FTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVATHLQWHIEQENIDT
                     RNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADAAKKHLRPVARQPLEVVR
                     DKLTAITDWTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGK
                     TRSIERINKALAFIAERENQQ"
     gene            20926..21001
                     /locus_tag="ACHKBL_002181"
     tRNA            20926..21001
                     /locus_tag="ACHKBL_002181"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20959..20961,aa:Val,seq:tac)
     gene            21006..21081
                     /locus_tag="ACHKBL_002182"
     tRNA            21006..21081
                     /locus_tag="ACHKBL_002182"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21039..21041,aa:Lys,seq:ttt)
     gene            complement(21192..22076)
                     /gene="xapR"
                     /locus_tag="ACHKBL_002183"
     CDS             complement(21192..22076)
                     /gene="xapR"
                     /locus_tag="ACHKBL_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator XapR"
                     /protein_id="HFO5819056.1"
                     /translation="MERVYRTDLKLLRYFLAVAEELHFGRAAARLNMSQPPLSIHIKE
                     LENQLGTQLFIRHSRSVVLTHAGKILMEESRRLLVNANNVLARVEQIGRGEAGRIELG
                     VVGTAMWGRMRPVMRRFLRENPNVEVLFREKMPAMQMALLERRELDAGIWRMATEPPT
                     GFTSLRLHESAFLVAMPEEHHLSSFSTVPLEALRDEYFVTMPPVYTDWDFLQRVCQQV
                     GFSPVVIREVNEPQTVLAMVSMGIGITLIADSYAQMNWPGVIFRPLKERIPADLYIVY
                     ETQQVTPALVKLLAALAQ"
BASE COUNT         5441 a         5916 c         5482 g         5242 t
ORIGIN      
        1 agatgcattg ctaatacacc atttatctat caatcccctt ttaaaattgt ggtttttcct
       61 caaaaacggc ctgctggtca tcagccttca ggttacctat catagaggtt taatccttat
      121 tcagagtcac ccgtgcacga aatattcaac atgctgctgg cggtcttcga tcgggcagcg
      181 ttaatgctta tctgcctgtt ctttctcatc cgtatccgtc tgtttcgcga actgttgcac
      241 aagtcggcgc actccccaaa agagttgctc gccgttaccg ccattttctc gctgttcgcc
      301 ctgttcagca cctggtccgg cgttcctgta gaaggttcgc tggtgaacgt acgtattatc
      361 gcggtgatgt caggcgggat tctgtttggc ccgtgggtgg gcatcattac cggcgtgatt
      421 gcgggtattc accgttattt aattgatatc ggtggcgtga cggcgatccc ctgctttatc
      481 accagcattc tggcgggttg tatatcgggc tggatcaacc tgaaaatccc caaagcacag
      541 cgctggcgcg tcggtattct cggcggcatg ttgtgtgaaa cgttgaccat gattctggtg
      601 attgtctggg caccaactac cgcattgggg atcgatatcg tctctaaaat cgggattcca
      661 atgatcctcg gcagcgtctg tatcggcttt attgtgttgc tggtacaaag tgttgagggc
      721 gaaaaagagg ccagcgccgc gcggcaggcc aagctggcgc tggatatcgc caacaaaacg
      781 ctgccgctgt ttcgccatgt caatagcgag tcattacgca aggtctgcga aattatccgc
      841 gatgacattc acgctgacgc ggtggcaatc accaataccg atcatgtgct ggcctacgtt
      901 ggcgtgggtg aacataacta tcagaatggc gatgacttca ttagcccgac tacccgccag
      961 gcgatgaatt atggaaaaat catcattaaa aacaatgatg aagcccaccg cacaccagag
     1021 attcattcca tgctggtgat cccattgtgg gagaaaggcg ttgtgaccgg aacgctgaaa
     1081 atttactact gccacgcgca tcagataacc tcgtcattac aggaaatggc ggtcggtctg
     1141 tcacaaatca tctccacgca actggaagtt tcgcgcgctg agcagctgcg tgaaatggca
     1201 aataaggcag agcttcgcgc cctgcaaagc aaaattaatc cccatttcct gtttaacgct
     1261 ttgaacgcta tttcatcgtc aatccgtctg aatccggata ccgctcgcca gctgatcttt
     1321 aatctgtcgc gttatctgcg ctataacatt gaattaaaag acgatgagca aatcgatatc
     1381 aaaaaagagc tgtatcaaat taaagactat attgccattg agcaggcccg ctttggcgac
     1441 aagctgacgg ttatctatga tattgatgaa gaggtgaatt gctgcattcc cagcctgctg
     1501 atccagccgt tggtggaaaa cgccattgtc cacggtatcc agccttgcaa aggtaaaggc
     1561 gttgtcacca tcagcgttgc agagtgcggt aatcgggtac gcattgcggt gcgggatacc
     1621 gggcacggca tcgatccaaa ggtgattgag cgggtcgaag cgaacgaaat gccgggcaat
     1681 aaaattggcc tgctgaatgt ccatcatcgc gtgaagttat tgtatggcga ggggctgcat
     1741 atccgccgcc tggaaccagg aacggaaatt gcgttttaca ttcctaacca acgcacccct
     1801 gtcgcctcac aggctacgtt attgctttga gccggagtga tattgtgaaa gtcatcattg
     1861 ttgaagacga attcctggca caacaggaac tgagctggct aattaaagag cacagccaga
     1921 tggagattgt cggcaccttt gacgacggtc tggacgtgtt gaagtttttg cagcataacc
     1981 gcgtcgacgc catttttctg gatatcaata ttccgtcgct ggatggtgtg ttactggcac
     2041 aaaacatcag ccagttcgcc cataaaccgt ttattgtgtt tatcaccgcg tggaaagaac
     2101 atgcggtaga agcgtttgaa ctggaggcgt ttgactacat tctcaaaccg tatcaggagt
     2161 cacgcattac cgggatgctg caaaaactgg aagcagcctg gcagcaacag cagaccagca
     2221 gtacgcctgc cgcgacggta acgcgtgaga atgacaccat taatctggtg aaagatgagc
     2281 gaatcatcgt cacgccaatt aacgatatct attacgccga agcgcacgag aaaatgacct
     2341 ttgtctatac gcggcgtgaa tcctacgtaa tgccgatgaa cattaccgaa ttttgcagca
     2401 aactgccgcc atcgcatttt ttccgctgcc atcgctcatt ttgtgtcaat ctgaacaaaa
     2461 tccgcgagat cgaaccgtgg tttaataaca cctatattct gcggctgaaa gatctggatt
     2521 ttgaagtgcc ggtcagccgc agcaaagtta aagaattccg ccagttaatg catctttaat
     2581 cgggaacttt catgaaagca ccaggcttgc ccgccgatca acaatttttc gccgatctgt
     2641 tcagcggcct ggtgcttaac ccgcaactac tggggcgcgt ctggtttgcc agccagcctg
     2701 cctcgttacc ggtgggcagt ttatgtattg attttccccg tctggatatc gtgctgcgcg
     2761 gcgaatacgg caatctgctg gaagcaaagc agcaacgtat ggttgaagga gaaatgctgt
     2821 ttattccggc gcgcgcggct aatttaccga tcaacaacaa accggtgatg ctgttaagcc
     2881 tggtgttcgc accgacctgg cttgggttat cgttttacga tagccgcacc acgtcgttgt
     2941 tgcatccggt tcgccagatc caacttccca gcctgcaacg cggcgaaggt gaagcaatgc
     3001 ttactgccct cacccatctt agccgttcgc cgctggagca gaatatcatt cagccgctgg
     3061 tgttaagttt gctgcatctt tgccgtaacg tggtgaatat gccgccgggc aattcgcagc
     3121 cgcgcggcga ttttctctat cacagcattt gtaactgggt tcaggataat tatgcccagc
     3181 cgctcacccg tgagagcgtg gcgcagtttt ttaatatcac gccaaatcat ctgtcaaaac
     3241 tgtttgctca gcatggaaca atgcgtttta tcgagtatgt tcgctgggta cgaatggcaa
     3301 aagcgaggat gattttgcaa aaatatcatc tgtcgattca tgaagtggcg cagcgttgcg
     3361 ggtttccgga tagcgactat ttttgtcgcg ttttccggcg tcagtttggt ctgacgccgg
     3421 gagagtacag cgcccgtttt cagggctaac gtcagaaggt taattctgtt tccagcagcg
     3481 tcaggatgct ttgtgcatct tgcgcggcaa acaacgactg gcggaagttt ttattcacca
     3541 gtttacgcgc cagctgcgag aagactttca catgattcat cccttcgtta gcgcccagtg
     3601 tcagcatgat caccagttcg acttcgccca tttctgactg ccagtcaacc ggtttcacca
     3661 gtcgggcaat gctgatactg gaatgacgga tccactgaga tttggtgtgc gggatcgcta
     3721 cgccaaaacc aacgccggtg gtaacaatct cttcccgctg ccagacatcc tcttccagct
     3781 cgaacggatg ttcagtgcgc ccgttaacgc cgaggttacc gcacaggaac tggatcgcct
     3841 gctctttatt gctaaaagcc tgatcaacaa agatattctc cagcgccagc agagggcgaa
     3901 cgtcttcttc cggcgtaaac gcggtgagta acgcttcaat ctcctgcgcg ctgcggcatt
     3961 cacatgcctg acgcgccagt tcccgacacg cctcgctatc cagttgacga agctggcttt
     4021 tcaccgccgg aatgcgcggg ctactcatac tcagctcgtc caggcccagc ccaagcagta
     4081 gcggcagata acggctttca ccgcccagtt caccgcaaat acctacccat ttaccccgct
     4141 gatgcgcagt ggtaacaatc tgctgcaaca tgcgcaggaa cgatggcgta atcgggttat
     4201 acagcggcga tacgcgcggg ttattacgat cgaccgcata cagatactgg gtcatatcgt
     4261 tggagccgat actgaagaaa tcgacctcat cgcagaagtg atcgatgatg tagcacaccg
     4321 acggcacttc caccatgata cccagcgtaa tcgtctctgc atgacgcagg ccatcgcgct
     4381 taagctcaac gatcgctttt tggatctcgc ccttcaccca taagatctga tcgaggctgt
     4441 gaaccattgg gatcatcaac tgggcgttgc cgaaactggc ggcgcgcaaa atggcccgca
     4501 gttgagtgcg gaacaggcca gcaaattccg ggtaaattcg taccgcacga tagccgagga
     4561 acgggttctc ttcctgggga atgttgaggt aaggaatgct tttatcgccg ccgatgtcca
     4621 ttgtgcggaa gataatcggc ttgtcgcccg cggccagtag cacctgctgg taggcttcaa
     4681 attgctcctg ctcgtccggc gcactgtcgc gatccatata gagcatttcg gtacgaaaca
     4741 gcccgacgcc ttccgcgccg ttggcaaacg cgccaggcgc ttccagcgcg gtgccaatat
     4801 tcgccgcgat atcaatgcgc ttgttatcac gggaataggc cagctgcgcg gcggcctgcg
     4861 cctgctgctg ctgacgttta tccgccagca tctgcgcaac ctgataataa ccgctcaccg
     4921 cgtcattcgg gttaatcgcc aacacgccgc actgggcgtc aagcaccgcc ggttgcccgg
     4981 catagcgcgc aatggcatcc agcggcaggc cgctcagtac cgggatcgcc gaggcgcggg
     5041 ccagaatcag ggtatgcgag gtgcgcccgg ttttctccag aatcatgccc gcaagatttt
     5101 ttaaattgag gctcaaaaac tggctagggg ttaaatcctc tgccaccaga atggtcggtt
     5161 tttccagcac cagattgttg cgcggtttta gttccggcca ggtgatatgc agtaactgtt
     5221 cgctgatatc gcgaatgtcg ctaacccgct cgcgcagata atcactggca gaagcagaaa
     5281 gtttggcgca aacctgctcc atattgctga tgatcgccgc ccccagcccc tgatgctgtt
     5341 ctgccatcag gtgacggata ttgcctgcaa attcatcatc ctgaatcagc gacaaatggg
     5401 cgctgaggat agttttgctt tcgccgtcac gctcacgcaa ttgctggttc agttgctcgg
     5461 caagcgttgc caggctgtgc tccagccggg tggaatcttg cgcactggcg gggatcgccc
     5521 ggtaactgtc gaggctgtcg ctctgtaaca gggtcagcgt acccacgccg acgccgcttg
     5581 ccagcacatt gccgtacagt aaatcaggat tcagacggct taatgaacgc ggcagcggat
     5641 gcgccgtcag ttctgcctgc gttggctgga cgctgtcgct gtcgataaag cgcacctgga
     5701 tgtactcttc cagcacgcgc cgcgcctgct cttcatcgct gccgctaatg ttcaggctgc
     5761 aaccgtcatt aaatagggtg ccggtgccaa tcagcgccag cgagcttttg gcatctgcct
     5821 ttgcgttctg gcgatggtta ataaaagtga tttcgctttg ccactggctg cactgttctt
     5881 taagttccca cgccggacga gcatgcagac cgtttggcaa aggacagaga aattgaatcg
     5941 ttaacataac cgctccgggt taatcaggag atcatctgaa atccgtcagt tgaacaaccg
     6001 tctcacgcgt aagacgttga ataagggcag ataaaagctg ctgcatctgc aaaatgtcac
     6061 ggcaatcggc aatcgatgcg gcgcaatgtc cgtggcgggt ggcaggcccc atcaccactg
     6121 tgggtacgcc agtgccggtt aagtgcaccg ccccaccgtc cgtgccaccg ttgctgaaca
     6181 tatccgcctg caacggcacg ccaatttctg ccgccacggt ttcaatccac gcggtaagtt
     6241 ttggcggcgc aatcagcgat ttatcgctta acaccagcat cggcccgtta ccaatctgac
     6301 gatggttagc cgcgccataa tcaaagtttt tcgcccagca ggcggtatca agcacaatgg
     6361 cgacgtccgg cgacaccgcg cgggtggcag tttgcccgcc gcgtaatccc acctcttcgc
     6421 tggaacttgc caccagccac acctccgcag gcagttcggc gtcgtgcagc tcacgtagta
     6481 acgtcaccag cagatagcaa ccgaggcgat catcaaaggc tttccccatc acccgctggt
     6541 gagggagaac ctgaaaagtt gtatcaaagg tgacgcgatc gcctggacga attcccgcct
     6601 gcatcacttc gtcatacgag cgcgcaccaa tgtccacgcg tatggtgctg acgtcattcc
     6661 cctgccggtc gccgtcaagc aggcctggaa ttttgcactc ttcacgggta gtgatacgca
     6721 ccgactgaag ctggcgggca gccatgcgta cattgccaac cggcaacaca tcgatcgccc
     6781 cttcgcggga gatgctgcgc accataaatc ccacttcgtc catatgcgca cagatcatca
     6841 ccttcggacc tgtcgactca ttgagtcgga tcagcaccga tcccagccca tcaaatcgca
     6901 cttctttttg caggcgatcc gcttcttcca gcaggatctg ccgtacttcc tgttccgagg
     6961 aggcgatcgc atctgcctcg ctcaacgctt ttaataacga taaatccatt atgcctctcc
     7021 cgtgagcaac actgttttcg gcatggcgta gagcacttct acgccttgcg gggtgaccag
     7081 cacaacatct tcgatgcgca cgcccccttg ccctggcaaa taaatccccg gctccacggt
     7141 cagtaacatg cctggctgta gcgtcgtggt gtcccgcggt gaaaaacgcg gatcctcatg
     7201 gacttcaata ccgatagcat gaccggtgtt atgaccgaaa tagtcgccat aaccggcttc
     7261 tgtaatcacc cggcgcgcgg cgtcgtcaac ctgctggcag cgtacgccgg ggcgaatcgc
     7321 ggagattgct gcgagctgtg cctgcaggac aatctgatag acgttaaaca gcgggtggga
     7381 ttcggcactt accccttcgc cattcaccag caaggtgcgc gtcatatcag agcagtagcc
     7441 ctgatacagc gcaccgaaat cgagagtgac aaactcgccc gctgcaacaa tcttgtcgct
     7501 ggctttgccg tgcggcagcg ccccacgcca gccgctggcg acaatggtat cgaaagaggc
     7561 tttttctgcg ccctgctggc gcataaacca ctccagttca gcggctatct cgcgctcgct
     7621 catccccgcc tgaataaagc ggcgaatatg ctctgcaccg cgatcggcaa tcccacaggc
     7681 gagacggatt ttctccacct cctctggcgt tttgagttgc cgcagcacat ccggcgtggc
     7741 gctaaccagt ttcgcattga gttcagactg ccagcggtgc gcggtttccc aactcacctg
     7801 ctggccttca aaaccgagcg tttgcaactg ctcatcggca aagatttgcc tgacgatggt
     7861 ggcaagcgtg tgcatcgcgt cgagcaaatg cagctggtaa ccttgcgtgc gggcttctac
     7921 atccgcgtaa tagcgcgagt ccaccagaat gtgcgcactt tcacggcaaa tcaccacata
     7981 gcctgatccg gtggagatcc ccagatgcgg ctgtttgttc tgccgtgagg agagaagcac
     8041 cgcatccagt tgttgcgcct taagccagtc gcgcagcgaa gcgagtaatg tcatcaggat
     8101 tgtccttctt acaggctatc gattaacaat ttgcctttac ggaacatcat cagacgcagg
     8161 aacaccacca tcagcgcagt tatgactgcc cccagcgcga tccccgccat gtagacgccg
     8221 aggttagtga ccagcggcca tgcccagata gcggactccg ggaaccattg cactgcgccc
     8281 agccagacag cggcggttga gccgacaatc gcgccgacca tataggacgg aatggcggtg
     8341 atggggcttt ccagcgcaaa cggaatcgca ccctcactga tccccataaa cgccaggaac
     8401 atggcggttt taccctgcgg ataaagctga gcactgaaca gacgtttgcc ggttaaacgg
     8461 cggtcgataa tggtcgccag acccagacca atcggcggaa taacaatagc gatggaacgc
     8521 gcggtaaccg gtaatacgtg gtcagtggta aagctgaagg caacgaaacc agctgctttg
     8581 ttgatcgggc caccaaggtc gattgctgtc gccgcggcga tccccatcgc gtacattagc
     8641 gcacccttct cacctgcggc agtcagcacg gtacggatac cgccgttgat ccagccacca
     8701 aagggggtga tgacgtagta catcgccagc atgacaaaaa tggcggaaag aatcggtagc
     8761 aaaaatgtgg ttttgaacgc taacaggaaa tccggtaact ggattttctg gttcatccac
     8821 ttcacaaggt aaccagcaac gatagaaata atcagggcac caatgaaggt ggacggcacc
     8881 ggcgaagagg tcgcccattg catcgtgctg ggatcgaagt tcagcagttg cgtcggctgg
     8941 gtagacatca acccaccgat aaaaccagcc gggaacgcca gtttgccacc gatagagttc
     9001 gccacaaatg cagcaaacat cggaatggca aaaccaaaca acacgccgcc gaaggactgc
     9061 gacaaccagg caaatttcag taaggagagg tcgaaaccgg agaatttccc gctattaaga
     9121 gcatccatga tgccgatatc agcaggaatt ttaagccagc tataagcaat cagctggctg
     9181 aaagcgagga tcacaccgcc cataattaag gtcggcacca tgcgtgaaat ccctgacatc
     9241 acatgctgcg ggagttcacc ccagaagctg gttttagagg cctgcggatt cttaactgcc
     9301 gctgccgcac cggatgcgcc aggcacaacg gttgcactgc gttttttaat ggccataaca
     9361 attatccctg tcgaatatta ctgttgttca gaggcaatca tctcttcgat ttctttgatg
     9421 atgcccgcag cgtttttaat tgcgtcctgc aaagtgattt cataaacgtc gcgtgattcg
     9481 aaacgttcgt tatcttccgg ggtaaccgcc acggagtgga tgatgatggt cgcttcggcg
     9541 atatcctgcg cggtcaggcg attctggatg ccgtccgcgc cctgagtttc aattttcact
     9601 tcatagcctg cttccaccgc cgcttcttcc agcgcctggg ctgccataaa ggtgtgagcc
     9661 agacccatcg ggcaggcaca taaagcaatc agtttcttac tcatcgttaa ttcctcgttc
     9721 agtgatagtt gacgagtgaa attatgaacg tgcaaaacaa atcgccgtta ccagacaaat
     9781 aatgcattta ctggagaatt aatcccgctg tacggaagtg tgacgcaggt cggccttgtg
     9841 aggggacaca aaggaaaatt gaattaactg tatgatttaa aagattatcg ggagagttac
     9901 ctcccgatat aacaggaagg atttagagaa tgtgacctaa ggtctggcgt aaatgtgcac
     9961 cggaaccaag aaggcccgga ttgtcatgga cgatgagata caccggaata tcatggacat
    10021 attctttaaa gcgcccttta tcttcaaatg cggcacggaa accagaggct ttgaagaact
    10081 caaggaagcg cggcacgata ccacccgcaa taaacacgcc gccaaatgtc ccgagattga
    10141 gcgccagatt gccgccaaaa cggcccataa tgacgcaaaa cagcgacaac gcgcggcggc
    10201 aatcggtgca gctgtcagcc agcgcgcgtt cggtaatatc ttttggcttg agattttctg
    10261 gcaggcggtt gtcggcttta acaattgcgc gatacaaatt caccagccca gggccagaaa
    10321 gcacgcgctc tgccgaaaca tgaccaattt ctgcacgcag tatttcgagg ataatgccct
    10381 cttcttcact attcggcgca aaatcaacgt gaccgccttc gcctggcaag cttacccaac
    10441 gcttatcgac atggaccaga tgcgcaaccc ctagccccgt tccggcaccg taaaccgcaa
    10501 taggcttacc ttctaccggt tctgcgccac caaactgaat cagatgctct tttttcagca
    10561 tcgggatcgc catcgataca gcggtaaaat cgttgataat ttccagatgg ctaaaaccga
    10621 gattcttttt catttcggca attgagaacg cccaggtatg gttggtcatt gccacccagt
    10681 caccggtaat tgggcaagcg atggcaatac agccgtcttg cacctcgatc ttatgttctt
    10741 caagataaac gcgaatgacc gcttcgaggc tggggtaatc aagccctgaa taggtcttag
    10801 cctgcgagat ttcaccactg gcaatatcac acagagcaag acgtgcgttg gtgccgccca
    10861 catcaccgac taatgcatac tttgtcattc ttcaactgct ccgctaaagt caaaataatt
    10921 ctttctcaca ctgtaaatac ctgggggcat aacaacaacg ccggaaaggc aggctccctg
    10981 taaatatcga tctgggtcac acaattactt tatcgtttca gcaccaattg cagcgatgcc
    11041 tttttgcaag ctgggcaaac taagtatctg accccgcata aggaatagaa catgctccat
    11101 ccgcgagcca gaaccatgtt gttattatcg ctccccgccg tggcaattgg gattgcgtcc
    11161 agtcttattc tgattgtggt gatgaaaatc gcctcggtat tacagaattt gctctggcaa
    11221 cgactgccgg gaactctggg gatagcccag gattcacctc tctggatcat cggtgtatta
    11281 acgctaacgg gtattgcagt ggggttggtt atccgtttca gccagggtca tgccggacca
    11341 gaccccgcct gtgaaccgct gatcggcgca ccagttccgc cctctgcgct accaggactt
    11401 atcgtagcat taattcttgg tcttgctggt ggcgtcagcc tggggccaga acatccgatc
    11461 atgaccgtca atatcgccct tgcggttgcc attggcgctc gtctgttacc gcgcgtcaac
    11521 cgaatggagt ggactatttt agcctctgcc ggaaccatcg gcgcgctgtt tggcacacct
    11581 gttgcggcgg cgttgatatt ttcgcaaacc ttaaatggca gtagtgaagt tccgctatgg
    11641 gatcgtcttt ttgcgccgtt aatggcggca gcagctggtg cactaactac cggattattt
    11701 ttccatcctc atttttcact gcccattgcg cattacggac agatgcaaat gaccgatatt
    11761 ctcagtggtg caattgtcgc ggcgattgcc atcgcagcag ggatggtcgc cgtatggtgc
    11821 ttaccacgat tgcacgcgat gatgcatcaa atgaaaaatc cggtgctcgt gctgggtatt
    11881 ggcggattta ttctcggtat tctgggggtt atgggtggac cagtttcgct gtttaaaggg
    11941 ctggatgaga tgcagcagat ggtggcaaat caggctttca gcaccagcga ttactttttg
    12001 ctggcggtga ttaaacttgc cgccctggtc gttgctgccg ccagtggctt tcgcggtggg
    12061 cgaatcttcc cggcagtgtt tgtcggcgtg gcattagggt tgatgctgca tgagcacgtc
    12121 cccgccgttc cagcggcaat aaccgtttcc tgcgctattc tcggcatcgt gctggtggta
    12181 acacgcgatg gctggttaag tctttttatg gcggcagtcg ttgtacccaa taccacattg
    12241 ctaccgctgc tctgtatcgt catgcttccg gcatggctgt tattagcagg taagccgatg
    12301 atgatggtca atcgtccgaa gcaacagccc ccccacgata acgtttagca aaaaatgctc
    12361 tcctttatga tttaagagtt atggcgatga tacggggctt tacctccccg taatattgcg
    12421 ttaacaggcc gctgacggct tcgatcgtga aggagaataa caatgttcag gtcactgttt
    12481 ctggcggccg ccctgatggc atttaccccg cttgcggcaa acgcaggtga aatcacccta
    12541 ctgccatcaa tcaaattaca aattggcgat cgcgatcatt acggtaatta ctgggacggt
    12601 ggtcactggc gcgaccgtga ctactggcat cgcaattatg aatggcgtaa aaaccgctgg
    12661 tggcgtcatg ataatggcta ccaccgtggc tgggataagc gtaaagcgta tgagcgtggc
    12721 tatcgtgaag gctggcgcga tcgtgacgat catcgcggaa aaggccgcgg acatggacac
    12781 cgccattaaa cacattcgcc aatggagcac aatgcctgat gcgacgctag tgcgtcttat
    12841 caggcctaca aaccggctct ttaacgcgta gggcggataa ggcgtttacg ccgcatccgc
    12901 caatagtgcc agatgcgacg ctcattcaac tacaatccca gcgccgtccc caccaacaac
    12961 cagatattca gcgccacgac cagcactaca atcacccagc ctgtctgttt tacgcgtttg
    13021 ctgttcacca gatcgcccat caacttgctg tcactggtga aaatcagcag tggaaccagc
    13081 gccagagcga taccaaaact taacagcacc tgactcataa ccagaatccg tgtcggatct
    13141 aatcccatca gaatgacaat aaatgacggc aacatggtaa cggtgcgacg cacccacagc
    13201 gggatatgga agcgaatgaa gccctgcatc accacctgcc ccgccagtgt ccccaccacc
    13261 gttgatgaca gtccggcagc aaccaggctt aatccaaaga ccgttgccgc agcatggctt
    13321 aacaacggtt gcagcgtcag gtaagcctca tcaagatcgg caacgccagt atgaccggaa
    13381 aagtggaacg ccgccgcagc cgtagccatc atcgccagat tgacaaaacc ggcaatagtc
    13441 atagcgatag ccacatccca tttggtggca gaataacgtt gttgacgcga accaccatgt
    13501 aaatgctgag tcagcgaaga gtgtaaataa atcacgtgtg gcataatcgt cgcccccaac
    13561 acacctgccg ccaggaagac tgcctccgag gtgggtaaac tcgggattac cattccttta
    13621 cccagttgcg ccaggttagg ctgggagaaa atcaactcga caatgtaagc cgcagcaaca
    13681 aacagcagta acccgccaat cactttctcc agcggttttt gccctcgacg ttgcagcatt
    13741 aaaatcagga aagtcgcgat ccccgtcagc accgcgccct gcaacagcga aacacccaga
    13801 atgagtttaa aaccgatcgc cgcaccaata aattccgcca ggtcggttgc catcgcaatg
    13861 atttctgcct gaacccaata gaaccacact acgggacgcg gatagtgatc gcgaatctgc
    13921 tccgccaggt ttttaccggt ggcaatcccc agtttggcag acagtatctg aatcagcatc
    13981 gccatcaggt tggcccaaac gacaacccac agtagctgat agccgaagct ggcacccgcc
    14041 tgaatattgg tcgcaaagtt accaggatcg atataaccaa tcgccgcaat gaacgcaggt
    14101 cccattaatg cgagcctcat cttgcgcgcc gcccgtccgc tgctactctc aacgcgatag
    14161 ttagtcatct tgtgcctcta aaacatagcc tttgctatgt ttcatgctat gtcaaacgag
    14221 aatgattatc aaattcattt aagtggattg tgatgatttc tttgatagac cagaattatg
    14281 acgacgaaaa gattgatgac ttgaatgttg tgattatgtt taatgttagc acatttacat
    14341 aactttcagc ttccatacac aacatagcag aaatgtatga cagctcacta tttttgaagc
    14401 ttgtcacagg acgtcattat agtgtgtgtc agatctcgtt ttccttaacc atgttacata
    14461 gaatgtgcac ggaaatttaa cctgcctcat atttggagca aatatggacc gcgtccttca
    14521 ttttgtactg gcacttgccg ttgttgcgat tctcgcactg ctggtaagca gcgaccgcaa
    14581 aaaaattcgt atccgttatg ttattcaact gcttgttatc gaagtgttac tggcgtggtt
    14641 cttcctgaac tccgacgttg gtttgggctt cgtgaaaggc ttctccgaaa tgtttgagaa
    14701 actgctcgga tttgccaacg aagggactaa cttcgtcttt ggtagcatga atgatcaagg
    14761 cctggcattc ttcttcctga aagtgctgtg cccaatcgtc tttatctctg cgctgatcgg
    14821 tattctccag cacattcgcg tgttgccggt gattatccgc gcaattggtt tcctgctctc
    14881 caaagtcaac ggtatgggca aactggaatc ctttaacgcc gtcagctccc tgattctggg
    14941 tcagtctgaa aactttattg cctataaaga tatccttggc aaaatctccc gtaaccgtat
    15001 gtacaccatg gcagcaacgg cgatgtccac cgtgtcgatg tccatcgttg gtgcatacat
    15061 gaccatgctg gaaccgaaat acgtcgttgc agcgctggta ctgaacatgt tcagcacctt
    15121 tatcgtgctg tcgctgatca acccttaccg tgttgatgcc agtgaagaaa acattcagat
    15181 gtccaacctg cacgaaggtc agagcttctt cgaaatgctg ggtgaataca ttctggcagg
    15241 tttcaaagtt gccattatcg ttgccgcaat gctgatcggc tttatcgccc tgatcgccgc
    15301 gctgaacgca ctgtttgcta ccgtgactgg ctggtttggc tacagcatct ccttccaggg
    15361 catcctgggc tacatcttct atccgattgc atgggtgatg ggtgttcctt ccagtgaagc
    15421 actgcaagtg ggcagtatca tggcgaccaa actggtttcc aacgagttcg ttgcgatgat
    15481 ggatctgcag aaaattgctt ccacgctctc tccgcgtgct gaaggcatca tctctgtgtt
    15541 cctggtttcc ttcgctaact tctcatcaat cgggattatc gcaggtgcag ttaaaggcct
    15601 gaatgaagag caaggtaacg tggtttctcg cttcggtctg aaactggttt acggctctac
    15661 cctggtgagt gtgctgtctg cgtcaatcgc agcactggtg ctgtaagacc atacataaaa
    15721 aagccgggga taattccccg gttttttacg cctgttaatc agctaatggc tgcgggcgac
    15781 caatcaaata cccttgcaga tattgcaccc cgagcttatg caatagcgcc tgctgctgtt
    15841 gcgtctcgac aaactccgcg accacactca atgacttcgc tttcgccaga tcggtaattg
    15901 atctcacaat catcgcatcc agcgtgttcg tgacgatatc tttcacaaag acgccatcaa
    15961 ttttgatgat atcagcctgc aaacgcttta accgttcgta gttggcatat ccggtgccaa
    16021 agtcatcaat cgcaatccgg aaaccaaact tatgcagctg ctcgatgttg tacatgctgc
    16081 tttctgcgtt agaaaacgcc tgctcctcgg tgatctcaag aatgaccgcc tgcggggaga
    16141 tgtgataacg cttaaacaga cgaataatcc gcccggcaat attcttttgc agcagcgtga
    16201 gcggcattaa attgactgaa aagcgcgggc cttttttgtc gcaaggatgt gttgccagcc
    16261 acttcaacag ggattccagc acttgcaaat caaaacgcgc gctaaggtta aactgagcaa
    16321 taaggggcag aaacttatcc ggggtcataa tgccgccgtc atatttcagt cgcgcgagga
    16381 tctcatcata accttcaccc tctttgttgc gaattggctg ggcgtagagc agcaaatcac
    16441 cctgatctaa cgccgtacga atggtattca gcaatagcac ctgtttagtg gtctgcccgg
    16501 aaaccatctc ctctctgcta tccagcgcca gcacatggtg atgtgcgcag gattgctccg
    16561 ccagccagct taactgcccc aacaagggtt gcagggtttc ctgatttcca tcaaaacgcc
    16621 cccaggcagc accatagccc atatccagcc cggtattgtt ccagtgaatt tgccgactat
    16681 tcaggatgtt aaccatatgc tggagtcgcc cttccgtttc cggtccactc agcactaaca
    16741 gcagttcact acccggcaat tgatacaact tttcgttttc ctgcatcaac ggcagcagcg
    16801 tacggtaaat tgatcggata cagtgaacgc gcatcattaa gccgtaatga cgactcataa
    16861 actcaagatt atcaatgcgc aggcagcaaa aactcttgcc cgcctcttgc tccggcgctt
    16921 gttctaacgc acgaaagttg ggtaggagcg ttaacggatc ggtcagcgcc tgcaaatgcc
    16981 agcggcgatt aagccattca ctgcgatgat aaatgcgcac catatagagc aggcaaacgc
    17041 taaaggaaat cagcaccgcg agaataaatg ccagcgaata ttcggttagt accccttgca
    17101 aaaagttctg gttgtaattc agcaggcaaa gcgttgaaac agcccaggtg agatttaaaa
    17161 acggatagcg aagcttaccg acaccgaggg taaagatgat aaagaaaaca ggtaccagat
    17221 aaccagcaat aaagtcattt tcatacggtg tgcacatcag aagtagcagc acgctaagag
    17281 ctgccagcca gcttaaggta aatgcgcgtt tctctttgct caacgacgga gcgatatccc
    17341 tacgccacaa tatctgtgca aagtggggac ttacaatcat gcgggcgaga tagtaaaaaa
    17401 gcatgttgta aatcagcact gcggtgaaaa ggcttagcaa atcaacgacc gtgaaaatgg
    17461 catccgcatc gccgaaaaag gtagatatct ttagcggaaa atcaaagaaa tttcccacaa
    17521 gatacatgct gcatttgatg ccaatcggcg ccaccaaacc aagccagaaa agacgttgcc
    17581 agacatatcg gctggtcaat ccataacgcc aacgcgtccc cagctgccag cgtaatattg
    17641 cgcaggcaca caacaccaca aacgtctggc tgccgagcat gaccgcggtc tgtaaaaaag
    17701 agagatgaaa gttccacaga ttggtgcaaa acatcccgca taaaacgggg acgacaccgc
    17761 gccagccaaa gataaacaag attgacagca ttacgcacag tggcatccac gccagaaaga
    17821 tataactgga gtggataatg gcaagtggcg aaataaaacg ggatagctga ataagtacca
    17881 cggtgagcgt aaacgctagt gtgaatatct tgatattttt tatcaggtta tgctccacaa
    17941 acatgagtac actactctca ctgatcgggg ttttcaggca cacaaataat aagtttattc
    18001 gcatcgcgaa gcaaacaggg gccagcctgt tgccgatttt tcatccaatg atgctttcac
    18061 caggcggcgc taacccggac gtagactgta ttacaaaagc ggcaaaaagc agagacaaaa
    18121 aacccccgct ttacagcgag ggtttgaaat ttggtggagc taagcgggat cgaaccgctg
    18181 acctcttgca tgccatgcaa gcgctctccc agctgagcta tagccccatc acgtaaagct
    18241 tgtcgagttg acgggcggca tcatatgaat tccgcccgaa tgtgtcaacg gcaaattgcc
    18301 acgtgtagtt tcaatcgctg aaaaatcagg caaatgaaca attttggaaa accgctcgca
    18361 ttcagtagtt attcatgtca cggtttcctg taaagtggtg ttataaaatg aactactaat
    18421 agacccacat acattcaggg aattgttatg ttcaaggaga ggatgacgcc agatgaactt
    18481 gccagactga ccggctatag ccgccagacc attaataaat gggtacgcaa ggaaggctgg
    18541 acaacgtcac caaaaccagg cgtccagggt ggcaaagctc gactggttca cgtcaatgaa
    18601 caggttcgtg aatatattcg caatgctgaa cgtccagaag gtcagggaga agcgccagcc
    18661 ctttctggtg atgcgccgct tgaagtgttg ctggtgacac tggcgaaaga gatgacgcca
    18721 gttgaacaaa aacagtttac atccttgctt ctgcgggaag ggattatcgg attgttacaa
    18781 cgcttaggga ttcgcgatag caaataatat gaaaagatta cgcaataaaa tgaccaccga
    18841 agaactggct gaatgcctcg gtgtggctaa acaaactgtt aaccgttgga tcagagaaaa
    18901 aggctggaaa acggaaaaat ttcccggcgt gaaaggcggt cgtgccagac ttattctggt
    18961 cgatacgcaa gtttgcgagt ttattcagaa cacgccagcc ttccataaca caccgatgct
    19021 tctggaagcc gaagaacccc ttgccgaata cgcaccgggt attcgcactc ctgcttatcg
    19081 gcaaatcatc agcgcaatag acaacatgac tcacagtgag caggaaaaag ttgcgcaatt
    19141 tttgtcacgc gaaggaattc gtaatttcct tgctcgtctc gacatagatg aatcagaata
    19201 aataaaaaac ggcaggggta tctctcctgc cgtttatctt ttatgcgcta attattgctg
    19261 attttcgcgt tcagcaataa aagccagcgc tttgttgata cgctcgatac tgcgggtctt
    19321 accgattgcg tgaacggtaa catccagcgc cggagactgc cccgcaccgg ttacggcgac
    19381 acgcagcggc ataccaactt tacccatacc cacttccagc tcatccgccg tcgcctgaat
    19441 agcgtgatga acgttttcag cggtccagtc agtaatcgca gtcagtttgt cacgaaccac
    19501 ttccagcggc tgacgcgcta ccggacgcag atgttttttc gcggcgtcgg catcgaactc
    19561 agcaaaatct tcgtagaaat aacggcagct ctgtgccatc tctttcagcg tcttgcagcg
    19621 ttcgcccagc agtttcacca gatcagccag ctgcgggccg ttacgggtat cgatattttc
    19681 ctgctcaatg tgccattgta agtgagtagc aacatactcc ggcggcagcg cgttaatgta
    19741 gtgatggttc agccacagca gcttgtcggt gttgaacgca ctggcagatt tgctgacggc
    19801 attcagagtg aagtatttga tcatctcttc acgagtgaag atttcctgat cgccgtggga
    19861 ccagcccaga cgcaccagat agttcagcag tgcttctggc aaataaccgt catcacgata
    19921 ctgcattacg ctgaccgccc cgtgacgttt ggacagtttt ttaccgtcat cgccgttgat
    19981 catagaaacg tgcgcgtaaa ccggtaccgg cgctttcagg gctttaagaa tgttgatctg
    20041 gcgcggcgtg ttgttgatat ggtcttcgcc acggataaca tgggtgattt ccatatccca
    20101 gtcatcaaca accacacaga agttataggt tggggaacca tcggtacggc gaatgatcag
    20161 atcatccagc tcctggttgc tgaactcaat cgggccacgg atctgatcgt caaaaacaac
    20221 agaaccttcc tgcgggttag caaaacgtac aacgcacggt tcatcatcag catgatgctc
    20281 atggctgtgg cggcagcgac cgtcataacg cggcttctca cctttcgcca tttgctcttc
    20341 gcgcagcgct tccaggcgct ctttagaaca atagcattta taagctgtgc cctcttccag
    20401 catctgatcg atcaccgcgt tgtagcgatc aaaacgtttg gtctggtagt acggaccttc
    20461 atcccactcc aggctcagcc agttcatgcc atccataatg gcttcgatag cttccggcgt
    20521 ggaacgctca agatcggtgt cttcaatacg cagcacgaac tcaccgccgt ggttacgtgc
    20581 aaaaagccag gagtaaagag cagtacgcgc gccgccaacg tgcagatagc ctgttgggct
    20641 tggcgcgaag cgagttttga ttttcatgaa atggccttac gtttagaaag atgccgacaa
    20701 ccggcaaatc ctggaaaaat tgagtgggcg atattctatc actcccgcct gattcctcaa
    20761 tgtagttcgg gttgcaacct tacgcattat tgttttcgtt gacaaattgc gcaacacggt
    20821 ctgttttgcg atcatcttgc ttaaatttac gacgaacgaa caatttcttt agaaaatgca
    20881 ttgactcatt ttgaactctc cctataatgc gactccacac agcgggggtg attagctcag
    20941 ctgggagagc acctccctta caaggagggg gtcggcggtt cgatcccgtc atcacccacc
    21001 acttcgggtc gttagctcag ttggtagagc agttgacttt taatcaattg gtcgcaggtt
    21061 cgaatcctgc acgacccacc aatgtaaaaa agcgccctaa aggcgctttt ttgctatctg
    21121 caatttgcga aattgcctga tgcgcttcgc ttatcagacc aactatgtcc ggcagttccc
    21181 gtgcctctca tctactgcgc caacgccgcc agcagtttaa ccagcgcggg cgtcacctgc
    21241 tgcgtttcat aaacaatata taagtctgcc gggatgcgtt ctttgagcgg acggaatatg
    21301 acaccgggcc agttcatctg cgcgtagctg tccgctatca atgtgatacc aatacccata
    21361 ctgaccatgg cgagtaccgt ttgcggttca ttaacttcgc gaataacaac cggtgaaaat
    21421 cccacctgct ggcaaactcg ctgcaaaaaa tcccagtcag tgtacacggg cggcattgta
    21481 acgaaatact cgtcacgtag cgcttccagc gggacggtgg aaaatgatga gagatgatgc
    21541 tcttcaggca tcgccaccag aaacgccgat tcatgcaacc gtaagctggt aaaaccagtc
    21601 ggtggttctg tcgccattcg ccagatcccg gcatcaagtt cgcggcgttc cagcaaggcc
    21661 atttgcatcg caggcatctt ttcgcgaaac agaacttcaa cgttaggatt ttctctaagg
    21721 aatcgccgca taaccgggcg catccgtccc cacattgccg ttcccacaac cccgagttca
    21781 atccgccctg cttctccccg acctatttgt tcaacccgag ccaatacatt atttgcattc
    21841 accagcaatc gacgcgattc ttccatcaag attttgcccg cgtgtgtcag tacgacgctg
    21901 cgcgaatggc gaataaaaag ctgcgtgccg agttgatttt ccagctcttt aatatgaatg
    21961 ctgagcggag gctgagacat atttaaacgc gctgctgcgc gaccaaaatg caactcttcc
    22021 gctacggcaa gaaaataacg gagcaactta agatctgttc tgtatacgcg ttccataatt
    22081 a
//
LOCUS       DAZFLD010000068        21715 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.068, whole genome shotgun sequence.
ACCESSION   DAZFLD010000068 DAZFLD010000000
VERSION     DAZFLD010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21715
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.068"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(120..305)
                     /locus_tag="ACHKBL_002184"
     CDS             complement(120..305)
                     /locus_tag="ACHKBL_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001468276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5819057.1"
                     /translation="MASGLICHGGFEPHAWGVASLMDVTVLVVASLLVWLLVNRENHY
                     ARGIPQDVQVLHKKAHH"
     gene            354..511
                     /locus_tag="ACHKBL_002185"
                     /pseudo
     CDS             354..511
                     /locus_tag="ACHKBL_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010835868.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            complement(526..601)
                     /locus_tag="ACHKBL_002186"
     tRNA            complement(526..601)
                     /locus_tag="ACHKBL_002186"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(566..568),aa:Thr,seq:cgt)
     gene            complement(716..1969)
                     /gene="proA"
                     /locus_tag="ACHKBL_002187"
     CDS             complement(716..1969)
                     /gene="proA"
                     /locus_tag="ACHKBL_002187"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HFO5819058.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLSEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGGVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCSLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESAEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADAGALAQLQTGPAKVVAVKTEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     SRGPMGLEALTTYKWIGIGDYTIRA"
     gene            complement(1981..3084)
                     /gene="proB"
                     /locus_tag="ACHKBL_002188"
     CDS             complement(1981..3084)
                     /gene="proB"
                     /locus_tag="ACHKBL_002188"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HFO5819059.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRSNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            3373..4428
                     /gene="phoE"
                     /locus_tag="ACHKBL_002189"
     CDS             3373..4428
                     /gene="phoE"
                     /locus_tag="ACHKBL_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HFO5819060.1"
                     /translation="MKKSTLALVVMGIVASVSVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNDSKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYVDVGATYYFNKNMSAFVDYKINQLESDNK
                     LNINNDDIVAVGMTYQF"
     gene            complement(4467..4868)
                     /gene="crl"
                     /locus_tag="ACHKBL_002190"
     CDS             complement(4467..4868)
                     /gene="crl"
                     /locus_tag="ACHKBL_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HFO5819061.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWTSVQIKDAEVVERLEHTLRE
                     FHEKLRELLATLNLKLEPADDFRDEPVKLTA"
     gene            complement(4926..6170)
                     /gene="frsA"
                     /locus_tag="ACHKBL_002191"
     CDS             complement(4926..6170)
                     /gene="frsA"
                     /locus_tag="ACHKBL_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HFO5819062.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFSHGAQLPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACAEEDPQLSGRYWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKVVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITDWIEKRLC"
     gene            complement(6262..6720)
                     /gene="gpt"
                     /locus_tag="ACHKBL_002192"
     CDS             complement(6262..6720)
                     /gene="gpt"
                     /locus_tag="ACHKBL_002192"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFO5819063.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQNTWIEQPWDMGVVFVPPISGR"
     gene            6981..8438
                     /gene="pepD"
                     /locus_tag="ACHKBL_002193"
     CDS             6981..8438
                     /gene="pepD"
                     /locus_tag="ACHKBL_002193"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HFO5819064.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            complement(8495..8847)
                     /gene="prfH"
                     /locus_tag="ACHKBL_002194"
                     /pseudo
     CDS             complement(8495..8847)
                     /gene="prfH"
                     /locus_tag="ACHKBL_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="similar to release factor 1 and 2; frameshifted;
                     incomplete; partial in the middle of a contig; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
     gene            complement(8843..9049)
                     /locus_tag="ACHKBL_002195"
                     /pseudo
     CDS             complement(8843..9049)
                     /locus_tag="ACHKBL_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
     gene            complement(9367..9819)
                     /gene="yafP"
                     /locus_tag="ACHKBL_002196"
     CDS             complement(9367..9819)
                     /gene="yafP"
                     /locus_tag="ACHKBL_002196"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO5819065.1"
                     /translation="MNNIQIRDYQPGDFQQLCAIFLRAVTMTASQHYSPQQIAAWAQI
                     DESRWKEKLAKSQVRVAVINAKLVGFITCVEHYIDMLFVDPEYTRRGVASALLKPLIK
                     SESELTVDASITAKPFFERYGFQTVKQQHVECRGAWFTNFSMRYKPQH"
     gene            complement(9816..10871)
                     /gene="dinB"
                     /locus_tag="ACHKBL_002197"
     CDS             complement(9816..10871)
                     /gene="dinB"
                     /locus_tag="ACHKBL_002197"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HFO5819066.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFNELQLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVPAFLQTLPLAKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVILLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            complement(10942..11727)
                     /gene="lafU"
                     /locus_tag="ACHKBL_002198"
     CDS             complement(10942..11727)
                     /gene="lafU"
                     /locus_tag="ACHKBL_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HFO5819067.1"
                     /translation="MTTIKLIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKISPSH
                     PPKPATVAVPEETEKKARDVNEKTALLKKKSATELGELATSINTIARDAHMEANLEME
                     IVPQGLRVLIKDDQNRNMFERGSAKIMPFFKTLLVELAPVFDSLDNKIIITGHTDAMA
                     YKNNIYNNWNLSGDRALSARRVLEEAGMPENKVMQVSAMADQMLLDAKNPQSAGNRRI
                     EIMVLTKSASDTLYQYFGQHGDKVVQPLVQKLDKQQVLSQRAR"
     gene            11699..13411
                     /locus_tag="ACHKBL_002199"
                     /pseudo
     CDS             11699..13411
                     /locus_tag="ACHKBL_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140833.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071978 - bacterial-type
                     flagellum-dependent swarming motility [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            complement(13629..14126)
                     /gene="rayT"
                     /locus_tag="ACHKBL_002200"
     CDS             complement(13629..14126)
                     /gene="rayT"
                     /locus_tag="ACHKBL_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="REP-associated tyrosine transposase RayT"
                     /protein_id="HFO5819068.1"
                     /translation="MSEYRRYYIKGGTWFFTVNLRNRRSQLLTTQYQTLRNAIIKVKQ
                     ARPFEINAWVVLPEHMHCIWTLPESDDDFSSRWREIKKQFTHACGLQNMWQPRFWEHA
                     IRNTKDYRHHVDYIYINPVKHGWVKQVSDWPFSTFHRDVAKGLYPIDWAGDVTDFSAG
                     ERIIL"
     gene            complement(14303..15052)
                     /gene="yafL"
                     /locus_tag="ACHKBL_002201"
     CDS             complement(14303..15052)
                     /gene="yafL"
                     /locus_tag="ACHKBL_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000093946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFO5819069.1"
                     /translation="MSSFLHGRFLHPGVFSLCVLLPLLASATTSHISFSYAARQRMQN
                     RARLLKQYQTHLKKQASYIVEGNAKSRRALRQHNREQIKQHPEWFPAPLKASDRRWQA
                     LAENNHFLSSDHLHNITEVAIHRLEQQLGKPYVWGGTRPDQGFDCSGLVFYAYNKILE
                     AKLPRTANEMYHYHRATIVANNDLRRGDLLFFHIHSREIADHMGVYLGDGQFIESPRT
                     GETIRVSRLAEPFWQDHFLGARRILTEETIL"
     gene            15364..16104
                     /gene="dpaA"
                     /locus_tag="ACHKBL_002202"
     CDS             15364..16104
                     /gene="dpaA"
                     /locus_tag="ACHKBL_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan meso-diaminopimelic acid protein
                     amidase"
                     /protein_id="HFO5819070.1"
                     /translation="MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPV
                     YIQIFKEERTLDLYVKMGEQYQLLDSYKICKYSGGLGPKQRQGDFKSPEGFYSVQRNQ
                     LKPDSRYYKAINIGFPNAYDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTG
                     ALVFGQPSVQVSIYPFRMTDANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVVN
                     GRYVVSKPLSHEVVQPQLASNYTLPEAK"
     gene            complement(16075..16842)
                     /gene="yafJ"
                     /locus_tag="ACHKBL_002203"
     CDS             complement(16075..16842)
                     /gene="yafJ"
                     /locus_tag="ACHKBL_002203"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HFO5819071.1"
                     /translation="MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGK
                     GCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRN
                     WTYAHNGQLTGYKSLETGNFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMAAVFKYIA
                     SLADELRQKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTT
                     PNDVVTVIATQPLTGNETWQKIMPGEWRLFCLGERVV"
     gene            complement(16958..17536)
                     /gene="lpcA"
                     /locus_tag="ACHKBL_002204"
     CDS             complement(16958..17536)
                     /gene="lpcA"
                     /locus_tag="ACHKBL_002204"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008968 - D-sedoheptulose 7-phosphate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HFO5819072.1"
                     /translation="MYQDLIRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAG
                     GKVLSCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGFNDIFS
                     RYVEAVGREGDVLLGISTSGNSANVIKAIAAAREKGMKVITLTGKDGGKMAGTADIEI
                     RVPHFGYADRIQEIHIKVIHILIQLIEKEMVK"
     gene            17776..20219
                     /gene="fadE"
                     /locus_tag="ACHKBL_002205"
                     /pseudo
     CDS             17776..20219
                     /gene="fadE"
                     /locus_tag="ACHKBL_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414756.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0033539 - fatty acid beta-oxidation using
                     acyl-CoA dehydrogenase [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase FadE"
     gene            complement(20262..20708)
                     /gene="ivy"
                     /locus_tag="ACHKBL_002206"
     CDS             complement(20262..20708)
                     /gene="ivy"
                     /locus_tag="ACHKBL_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ivy family C-type lysozyme inhibitor"
                     /protein_id="HFO5819073.1"
                     /translation="MFKAITTVAALVIATSAMAQDDLTISSLAKGETTKAAFNQMVQG
                     HKLPAWVMKGGTYTPAQTVTLGDETYQVMSACKPHDCGSQRIAVMWSEKSNQMTGLFS
                     TIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLENHPDGFNFK"
     gene            20889..21659
                     /gene="yafV"
                     /locus_tag="ACHKBL_002207"
     CDS             20889..21659
                     /gene="yafV"
                     /locus_tag="ACHKBL_002207"
                     /EC_number="3.5.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutaramate amidase"
                     /protein_id="HFO5819074.1"
                     /translation="MPGLKITLLQQPLVWMDGPANLRHFDRQLEGITGRDVIVLPEMF
                     TSGFAMEAAASSLAQDDVVNWMTAKAQQCNALIAGSVALQTESGSVNRFLLVEPGGTV
                     HFYDKRHLFRMADEHLHYKAGNERVIVEWRGWRILPLVCYDLRFPVWSRNLNDYDLAL
                     YVANWPAPRSLHWQALLTARAIENQAYVAGCNRVGSDGNGCHYRGDSRVINPQGEIIA
                     TADAHQATRIDAELSMMALREYREKFPAWRDADEFRLW"
BASE COUNT         5239 a         5637 c         5588 g         5251 t
ORIGIN      
        1 tggttaccta atcgccaatt aatataagct ccattatttc tccatatttt catgttagaa
       61 gtgacaagga gaaatgtaat gtttctctca gagaatataa atcattcctt ttgcccacct
      121 taatggtggg cttttttatg caaaacctga acgtcctgtg gtattcctct ggcataatga
      181 ttttcgcgat taaccaacaa ccagaccagc aaagacgcca ccacaagcac agtaacatcc
      241 atcaggcttg ccacacccca tgcgtgcggc tcaaagccgc catggcatat taatccgctt
      301 gccattttcg aacagctgat tggtttggtc cggtgtttcc ctgcttgcac ttaaaaatga
      361 gcggatggga aactctcgaa atggttcgtc gatatgctca cctcgcacct aatcacctta
      421 ccgaacacgc acggtaaata gattcgatcc tgaacccatc ggtcccaaat ctgtcccaat
      481 aaaaaataag gaaggtatta gtgatgtgta acttattgat ttaaatggtg ccgataagag
      541 gagtcgaacc tccgaccttc gcattacgaa tgcgctgctc taccaactga gctatatcgg
      601 ccctgaaagg acatgttcac gaacgtgaat cacggtggac aaggttaaaa ctaaccgggc
      661 gatgcgtcaa tggccttgtg aatcaaatgg ctacttttgc atcacccggt tttatttacg
      721 cacgaatggt gtaatcacca atgccgatcc acttgtaagt ggtcagtgct tccagcccca
      781 ttgggccacg cgagtggagt ttttgtgtgc ttaccgccac ttccgcaccc agtccaaact
      841 ggccgccgtc ggtaaaacgc gtagaggcgt taacgtaaac agcggaggaa tctacttcgt
      901 taacaaaacg ctgggcgttg cgcatatcgc gggtcaggat cgcatcggag tgttgcgtgc
      961 cgtgttcacg aatatgggcg atggcatcgt caagatcgct gacgattttg acgttcaaat
     1021 ctaatgacag aaactcatcg tcatactctt cggttttaac agcaaccacc ttcgcggggc
     1081 ctgtctgcaa ctgcgccagt gcacctgcat cagcgtgtaa cgtcacgcca ctttccgcca
     1141 tttgtttgct taatgcgggc aggaaactat cggcgatgtt tttattcacc agcaacgttt
     1201 caaccgtatt acatgtgctc ggtcgctgag ttttcgcgtt gacgatcact ttcagggctt
     1261 cagcgatctc tgcactttca tcaacgtaaa tatggcatac gcctatacca cctgtgatca
     1321 ccgggatcgt cgactgttcg cggcacagtt tatgcaggcc agcgccaccg cgcgggatca
     1381 gcatgtcgat gtatttatcc atacgcagca tttcactgac cagcgcgcgg tcaggattat
     1441 caatcgcctg cacggcaccc gccggtaagc tgcaggattt cagggcgtcc tgaatcaccg
     1501 ccaccgttgc agcgttagtg cgacacgttt ctttgccgcc gcgcaggatc accgcgttac
     1561 ctgttttcag gcacagcgaa gcgacatcta ccgtcacgtt cgggcgcgct tcataaatca
     1621 cgccaatcac ccccagcggc acgcgacggc gctccagacg cagaccactg tccagtacgc
     1681 cgccatcgat tacctgcccc accggatcgg cgagattaca cacctgacgc acatcgtcgg
     1741 cgatgccttt cagccgtgcg ggcgtcagtg ccagacggtc aagcatcgct tcgctaaggc
     1801 cattggcacg cgcgtcagca acatcctggg cgttagcgtt gaggatgatt tcgctttgtg
     1861 cttccagttc atcggcgatt ttttccagca cacgattttt ttcgcggctg gagagttgcg
     1921 ctaatttata cgaggcttgc tttgcggcaa tgcccatttg ttccagcatc ggccagctcc
     1981 ttaacgggta atcatgtcat cacggtgaac ggcaaccggg ccatattcat atcccagtat
     2041 tgcatcaatt tcttgcgagt ggtgtccggc aatgcggcgt aatgcatcgc tgttgtaacg
     2101 actgacgccg tgggcgatat cgcgaccttc gaggttgcaa atgcggatga cttcaccacg
     2161 cgagaaattg ccagttacgc tcttaatgcc tttcggcaac agggagctgc cgcgttcaag
     2221 aatggcggcg gttgcccctt catctaccgt gatttcaccc gccggcggcg caccgaaaat
     2281 ccagcgttta cggttttcaa gcggagtcgc ctgggcatgg aacagcgtac cgacggaaat
     2341 gccttccatc acatcaccaa taacgcccgg cttgctgccc gcggcaataa tggtgtcgat
     2401 acccgcacgg caagccacgt ccgcggcctg caatttggtg ctcataccgc cagttccgag
     2461 gcctgaaacg ctatcaccgg caatcgcgcg cagtgcgtca tcaatgccgt acacatcttt
     2521 aatcagttct gcctgcggat tgctgcgcgg gtcagcggta tacaaacctt tttgatcggt
     2581 cagcagcaac agtttatcgg cacccgccag aatcgccgcc agcgcagaaa ggttatcgtt
     2641 atcaccgacc ttaatctctg ccgtagcgac agcatcgttc tcattaatta ccggaacgat
     2701 attgttatcg agcaacgcac gcagggtatc gcgggcgttc aggaagcgtt cacgatcttc
     2761 catatcagca cgggtcagca acatctgccc gacatgaatg ccgtaaattg aaaacagctg
     2821 ttcccacagt tgaatcagtc gactctgccc taccgccgcc agcagctgtt ttgaagcgat
     2881 agtcgctggc aattccgggt accccaggtg ctcacgtccg gcggcaatcg cacccgacgt
     2941 cacaataaca atccgatgcc cggcggcatg taactgcgcg cactggcgaa caagttcaac
     3001 gatatgggca cggttcaggc ggcgcgatcc gcctgttagc acactggtgc cgagttttac
     3061 caccagcgtc tggctgtcac tcatgattct ctgccattca attttaggaa aaaatgatat
     3121 caaacgaacg ttttagcagg actgtcgtcg gttgccaacc atctgcgagc aaagcatggc
     3181 gttttgttgc gcgggatcag caagcgtagc ggcagttgtt tacgctttta ttacagattt
     3241 aataaattac cacattttaa gaatattatt aatctgtaat atatctttaa caatctcagg
     3301 ttaaaaactt tcctgttttc aacgggactc tcccgctgaa tattcgcgcg ttaattaaaa
     3361 tcaggaatga aaatgaaaaa gagcactctg gcattagtgg tgatgggcat tgtggcatct
     3421 gtatccgtac aggccgcaga aatatataac aaagacggta ataaactgga tgtctatggc
     3481 aaagttaaag ccatgcatta tatgagtgat aatgacagta aagatggcga ccagagttat
     3541 atccgttttg gttttaaagg cgaaacacaa attaacgatc aattgaccgg ttatggtcgc
     3601 tgggaagcag agtttgccgg taataaagca gagagtgata ctgcacagca aaaaacacgt
     3661 ctcgcttttg ccgggttgaa atataaagat ttgggttctt tcgattatgg tcgtaacctg
     3721 ggcgcgttgt atgacgtgga agcctggacc gatatgttcc cggaatttgg tggcgactcc
     3781 tcggcgcaga ccgacaactt tatgaccaaa cgcgccagcg gtctggcgac gtatcggaac
     3841 accgacttct tcggcgttat cgatggcctg aacttaaccc tgcaatatca agggaaaaac
     3901 gaaaaccgcg acgttaaaaa gcaaaacggc gatggcttcg gcacgtcatt gacatatgac
     3961 tttggtggca gcgatttcgc catcagtgga gcctatacca actctgatcg caccaacgag
     4021 cagaacctgc aaagccgtgg cacaggcaag cgtgcagaag catgggcaac aggtctgaaa
     4081 tacgatgcca ataatattta tctggcaact ttctattctg aaacacgcaa aatgacgcca
     4141 ataactggcg gctttgccaa taagacgcag aactttgaag cggtcgctca ataccagttt
     4201 gactttggtc tgcgtccatc gctgggttat gtcttatcga aagggaaaga tattgagggc
     4261 atcggtgatg aagatctggt caattatgtc gatgttggtg ctacgtatta tttcaacaaa
     4321 aatatgtcag cgtttgttga ttataaaatc aaccaactgg aaagcgataa caaattaaat
     4381 attaataatg atgatattgt cgcggttggg atgacgtatc agttttaatg aatattgccg
     4441 gatgtgatgc atccggcaca tttcattcac gccgttaact tcaccggttc atcacgaaaa
     4501 tcatctgccg gttccagctt cagattcagc gtcgccagca gctcacgcag cttctcgtga
     4561 aattcacgca gggtgtgctc cagtcgttca accacttcgg catcttttat ctgaacactc
     4621 gtccagtcgc ctgctttatc gaacagacca aactggtaac tgtaggtaaa acgggattcc
     4681 tgcgcttcca gttccatcca ccagccccag aactcacgca cttccggtgc cggtttcacg
     4741 ttgacgcata cagccagaca atcgaaaaag aatcgattat cttcgcactt accttcacga
     4801 atatacgggc ctagtgcggt aaattttttg atcaatctgc tcttcgggtg tccactcggt
     4861 aacgtcattg caatctcctg ttgtgatgca actgttttac caaattggta aaatttagca
     4921 aatttttaac acaaacgttt ttcgatccag tctgtgattt cctgaaggcc tttgtcaaaa
     4981 ttccgataca ccgggttaaa tgggatctct aataatttac cgtcagcaga tgacgacgtg
     5041 attaagcgtg agtcctcttc cgggctgaac ggatcgttct tccagtagcc tgataacatt
     5101 ggcgttgggc agcgacgtcc cagcaatcct tgcactttta atgaatagcg attcaactcc
     5161 actcgcaacg cttcatcgga agcatcatgc atccctaaac gactggccag aacgtcaaga
     5221 tacatttccg gcacctgttg ctggcactta aaatcactca acagggtatg aactaccgga
     5281 ccaagacagg caaccacttt cagacgcggc gattcaaggt atgccagacg cacggcaacg
     5341 ttagcgccga aacggaaacc aaaggccgcg acgcgagtgt gatccaccca cggtacgtta
     5401 ggcagcgcct ttaaaacgtg ctgatgcaac aggctggagt cctgggtgag cttccatttt
     5461 gaagaaaagc ccaccgacgg catatcaata gtcagcatag caatgccgcg cggcgcaaaa
     5521 taacgttcat acaggctgta atagtccgtc tgcatcgcat ccagaccacc acacattaat
     5581 actgtcggga acgggccatc gcctttcggc atatgcaaaa agccggtgat gggcgcaccg
     5641 ccgggtacgg taaactccat ctgccgcatc gtgcccggca gacgctgagc ggcctcttca
     5701 taggcgcggt ttgacaaagc ctgcgcttgc tcggccaggt catctccttt cagatgagga
     5761 taggcagcaa tgttgtacaa cgtagccgca tgcagccagt atcgaccgct gagttgcgga
     5821 tcttcttccg cacaggcttt ttgttgccac accatcgcct gggtagccca ctcataaatc
     5881 cagttgccgc cgcggtagcc aatcaccgta tcgtataaat catcgtcggt acgttcggca
     5941 tcgctcatca caatacgcgc ctggacgtcg aggatttcac gggggtcaat gccgcgccag
     6001 atccacatca gacggttaat catgcgatac cagtgaggga tggttttacc atcaagggcc
     6061 gactgcacag gcagttgtgc gccgtgacta aagcggcgga ctagcgtcga ggtttcagga
     6121 tgtttaaagc ggggtttgaa cagggtttcg ctcaggtttg cctgtgtcat ggatgcagcc
     6181 tccagaatac ttactggaaa ctattgtaac ccgcctgaag ttaaaaagaa caacgcccgg
     6241 cagtgccagg cgttgaaaag attagcgacc ggagattggc ggcacgaata cgacgcccat
     6301 atcccacggc tgttcgatcc aggtattttg cgggatatca acaacataat catcaaccag
     6361 cggacgacca gccggttttg cgaagatggt gacaaagtgc gcttttggat acatttcacg
     6421 aatcgcaacc gcagtaccac cggtatctac caggtcatca ataacgatga agccttcgcc
     6481 atcgccttct gcgcgtttca gcactttaag ctcgcgctgg ttgtcgtgat cgtagctgga
     6541 aatacaaacg gtatcgacat gacgaatacc cagttcacgc gccagtaacg cacccggtac
     6601 cagaccgcca cggcttacgg caataatgcc tttccattgt tcagaaggca tcagtcggct
     6661 tgcgagttta cgtgcatgga tctgcaacat gtcccaggtg acgatgtatt tttcgctcat
     6721 gtgaagtgtc ccagcctgtt tatctacggc ttaaaaagtg ttcgagggga aaataggttg
     6781 cgcgagatta tagagatctg gcgcactaaa aaccagtatt tcacatgagt ccgcgtcttt
     6841 ttacgcactg cctctccctg acgcgggata aagtggtatt ctcaaacata tctcgcaagc
     6901 ctgtcttgtg ttgacaacat tttctgctaa ccctgtgacc tgcaatactg ttttgcgggt
     6961 gatcgacaag gagacttaac gtgtctgaac tgtctcaatt atctccacag ccgctgtggg
     7021 atatttttgc caaaatctgt tctattcctc acccgtccta tcatgaagag caactcgctg
     7081 aatacattgt tggttgggca aaagagaaag gtttccatgt cgaacgcgat caggtaggta
     7141 atatcctgat tcgtaaacct gctaccgcag gtatggaaaa tcgtaaaccg gtcgtcttgc
     7201 aggcccacct cgatatggtg ccgcaaaaaa ataacgacac cgtgcatgac ttcaccaaag
     7261 atcctatcca gccttatatt gatggcgaat gggttaaagc acgcggcacc acgctgggtg
     7321 cggataacgg cattggtatg gcctctgcac tggcggttct ggctgacgaa aacgtggttc
     7381 acggcccgct ggaagtgctg ctgaccatga ccgaagaagc cggtatggac ggtgcttttg
     7441 gcttacagag caactggttg caggctgata ttctgattaa caccgactcc gaagaagaag
     7501 gtgaaatcta catgggttgt gcgggaggta tcgacttcac ctccaacctg catttagatc
     7561 gtgaagcggt tccagctggc tttgaaacct tcaagttaac cttaaaaggc ctgaaaggcg
     7621 gtcactccgg cggtgaaatc cacgttggcc tgggtaatgc caacaaactg ctggtgcgct
     7681 tcctggcggg tcatgcagaa gaactggacc tgcgccttat cgatttcaac ggcggcacac
     7741 tgcgtaacgc catcccgcgt gaagcctttg cgaccattgc tgtcgcagct gataaagtcg
     7801 acgctttgaa atctctggtg aatacctatc aggagatcct gaaaaacgag ctggcagaga
     7861 aagagaaaaa tctggccttg ttgctggact ctgttgcgaa cgataaagct gccctgattg
     7921 cgaaatctcg cgataccttt attcgtctgc tgaacgccac cccgaacggt gtgatccgca
     7981 actccgacgt ggcaaaaggt gtggtcgaaa cctccctgaa cgtcggtgtg gtgaccatga
     8041 ctgacaataa cgtagaaatt cactgcctga tccgttctct gatcgacagc ggtaaagact
     8101 acgtggtgag catgctggat tcgctgggta aactggctgg cgcgaaaacc gaagcgaaag
     8161 gcgcatatcc tggctggcag ccggacgcta attctccggt gatgcatctg gtacgtgaaa
     8221 cctatcagcg cctgttcaac aagacgccga acatccagat tatccacgcg ggcctggaat
     8281 gtggtctgtt caaaaaaccg tatccggaaa tggacatggt ttctatcggg ccaaccatca
     8341 ccggtccaca ctctccggat gagcaagttc acatcgaaag cgtaggtcat tactggacac
     8401 tactgactga actgctgaaa gaaattccgg cgaagtaatt atttgatttg ctgccggatg
     8461 gcgtttaatc gccttccggc agtttcatcc ttcattatcc ttcgataaaa gccatccctg
     8521 tgaacgttcg tcgcgggttg ccacgttcaa tctggtgatg gaacattcgc cgctgcgatt
     8581 tcagcgccgc gctattttcc tgttgctgtt gttccagctt ccaggcaatc agcaatcgcg
     8641 ccagccgctt gttagcatgc ccagaaacca gtttttgcgc ccatgatgag gccgatacgg
     8701 actcggacaa atacactgaa tagtgccgca ccaactttcg cttaatgccc atgcattatc
     8761 gccatccaga gaaatcagcg ccgaacgcag cgtgtcagag tagcggcccg tttctgtttc
     8821 cagcaccgtt accgcgacgt cttgccgggt aaaaacgacc aacagagaag aacgctgcgc
     8881 caatcgggta gccgcgtccg ggatgtaaat ctggcatccc cacaacgcgc tgcatgttgg
     8941 gtaaatttgc cgtggtgtgt aattgttgga tcgctaaact ctcgatccac aggtcatcag
     9001 atgcgatggt aaataccgca tcggataagg gacgaatata tttgcccatg tatcattcca
     9061 tttatactcg tcatacttca agttgcatgt actgcgaccg cgttcgttca ccccagtcac
     9121 ttactttagt aagctcctgg ggattcactc gcttgtcgcc ttcctgcaac tcgaattatt
     9181 tagagtatat aaatataaaa atcaggaaat ggcagacgaa tccagggcaa gaaaaaaaga
     9241 attaatcctg gaaaggtctg caaagagtca ttgataaagg atttgtcatg atttacctcc
     9301 ttgcagtttg tggaataaga aaacgcgcac ataccaggcg ggtgttattt tcattgcaag
     9361 ctggatttaa tgttgcggtt tatatcgcat agaaaaatta gtaaaccacg ctcccctgca
     9421 ttcaacgtgc tgctgcttaa ctgtctgaaa accataacgt tcaaaaaagg gttttgcggt
     9481 tatgctggcg tccaccgtaa gttcggattc agacttaatc aaaggtttta acaaggcgct
     9541 ggcaaccccg cggcgggtgt attcagggtc aacaaataac atatcgatat agtgttcaac
     9601 gcaggtaata aagccgacca gttttgcatt aatgactgca acccgcactt gtgatttcgc
     9661 gagtttctcc ttccagcgag attcgtcaat ctgcgcccag gcggcaattt gttgtggtga
     9721 ataatgctga ctggcggtca tcgtaaccgc tctaaggaaa atagcgcata gttgctgaaa
     9781 atcgccaggc tgatagtctc ttatttgtat gttattcata atcccaacac cagttgtctt
     9841 tccatttgcg ggtcaagtaa cgtcacatgc agccccacca gtcgcacacc gcgcccgccg
     9901 cggcgttcat cccaggtttt acgcgcagtg gcgattagat cagctttatt cagccgtggc
     9961 cagacgtgct cctgagtagt ttgctgaaaa tcgtcgaact ttaatttcac tccctgacgg
    10021 gcgatcagta aatcaggttt cacctttgcc agacggcgtt caagttcagg atacagccgc
    10081 tcgataatcg cttcacattc agaccagtga tgaatatctt ccgccatcgt gcgttccacg
    10141 ccgacggatt ttcgcaaccg ttcactgtta acgtcgcgct cgtcaatccc ctgactacgc
    10201 tcccacaaaa tgcggccaaa tttgccaaag cgtttaagca gaatcaccag atcacacttt
    10261 tgcacatcac cgcaggtccg cagccccatc gcttccagtt ttgccgccga gactttgccg
    10321 acgccgggaa tttttgccag cggtaaggtt tgcagaaatg ccggaacttc tgccggcgta
    10381 atcacaaact gaccgttggg tttattcatg tcggaggcga ttttggcgag aaactttact
    10441 ggtgccacgc ccgcagatgc cgtcagttgc agctcgttga agatcgtctg gcggatttcc
    10501 tgggcgatga gggtcgcaga accgtggcaa tggacgctat cagtgacatc gagataggct
    10561 tcatccagtg acaacggttc aatacgcgag gtgtagcgcg agaagatttc acggatatga
    10621 tttgaggctt ctttgtaggc gtcaaagcgc cccggaagca aggtgagatg cgggcataat
    10681 ttgagcgcca tccctgtcgg catagcgcta cgtacgccaa atttacgcgc gggataattg
    10741 gcggtgctga ttaccccccg tcgttcgcgg ctgccgccaa tagcaatagg gatatcgcgc
    10801 agggcgggat tgtcgcgcat ctccaccgct gcgaaaaagc agtccatatc cacatgaatg
    10861 attttacgca ttgctcacct ctcaacactg gtaaagtata cagtgatttc agggtttgag
    10921 aaatgcgtaa agattcagca tttaacgcgc tcgctgcgaa agcacctgct gcttatccag
    10981 cttttgcacc agcggctgca ccactttatc cccatgctga ccaaagtatt gatacagcgt
    11041 atctgacgca cttttggtca gcaccataat ctcaatgcgc cggttgcccg cgctttgcgg
    11101 atttttggca tccagcagca tctggtccgc cattgcgctt acctgcatca ctttattttc
    11161 cggcattccg gcctcttcca gcacccgacg agccgaaagc gcgcgatcgc ccgaaaggtt
    11221 ccagttgttg tagatattgt ttttgtaggc catcgcatcg gtatgcccgg taataataat
    11281 tttgttatcg agcgagtcga acactggcgc gagctccacc agcagcgttt taaagaacgg
    11341 cataatcttc gcgctgccgc gttcaaacat attgcggttc tggtcatctt taatcagcac
    11401 gcgcagtccc tggggaacaa tctccatctc cagattcgct tccatatgtg catcacgggc
    11461 aatggtgttg atactggtcg ccagctcgcc aagctcggtg gcggatttct tcttcagcag
    11521 cgccgttttc tcgttgacgt cgcgcgcttt tttctccgtc tcttccggca ccgccacggt
    11581 ggcaggtttt ggcggatgag acgggctgat tttatttaac ggcgacaacc caccaccgtt
    11641 aaaaatcgac tgcccgtgca gcgcggcgat aatgctttca cgctcggatt tactgacgct
    11701 attgacaatc aactttatcg tcgtcaccaa aggggccgag cgtatttccg aggtttccgc
    11761 ccgctttacc ttagacgcga tgcccggtaa gcagatggcg attgacgccg atcttaacgc
    11821 cgggttgatc aaccaggcgc aggcgcaaac ccggcgtaaa gatgttgcca gcgaggccga
    11881 tttctacggc gcaatggacg gggcatcgaa gtttgtgcgc ggggacgcca tcgccgggat
    11941 gatgattctg gcgatcaacc tgatcggcgg cgtctgtatc gggatcttca aatacaacct
    12001 gagcgccgac gccgccttcc agcaatatgt gctgatgacc atcggcgacg ggctggtggc
    12061 gcagatccct tccctgctgc tctccaccgc ggcggcgatt atcgtcaccc gcgtcagcga
    12121 caacggcgat atcgcccatg acgtgcgcca ccagctgctg gcaagcccgt cggtgctcta
    12181 caccgccacc ggaattatgt ttgtactggc ggtggtgccg ggaatgccgc acctgccgtt
    12241 tttgctgttc agcgccctgc ttggctttac cggctggcgg atgagcaaac gcccgcaggc
    12301 ggcggaggcg gaggagaaaa gtcttgaaac gctaacccgc accatcactg aaaccagcga
    12361 gcaacaggtc agttgggaaa ccattccgct gatcgagcct atcagcttaa gcctcggcta
    12421 caagctggtg gcgctggtcg ataaagcgca ggggaaccca ctcacccagc ggattcgcgg
    12481 cgtgcggcag gtgatatccg acggcaacgg cgtgctgctg ccggagatcc gtattcggga
    12541 aaacttccgc ctcaagccca gccagtacgc catattcatc aacggcatta aggccgatga
    12601 agcggatatt ccggcagata aactgatggc cctgccctcc agcgaaacct acggcgagat
    12661 tgacggcgta ctggggaatg acccggcata tgggatgccg gtcacctgga ttcagcctgc
    12721 gcagaaagcg aaggcgctga atatggggta tcaggtgatc gacagcgcca gcgtgattgc
    12781 cacccatgtg aataagattg tgcgcagcta tattcctgat ttatttaact atgacgatat
    12841 tacgcagttg cataaccgcc tgtcgtcgat ggcaccgcga ctggcggaag acttaagcgc
    12901 agcgctcaat tacagccagt tgctgaaagt gtatcgcgcg ctgctgaccg aaggcgtttc
    12961 cctgcgcgat attgtcacca tcgccaccgt gctggtcgcc agtagtgcgg tgactaaaga
    13021 tcatatcctg ctggcggcag atgtgcgcct ggcgctgcgg cgcagtatca cccatccgtt
    13081 cgttcgcaag caggagctga cggtgtatac gctgaataat gagctggaaa atctgctgac
    13141 caacgtggtg aatcaggcgc aacaggccgg caaagtgatg ctcgacagcg tgccggtgga
    13201 cccgaatatg cttaaccagt tccagagtac gatgccacag gtgaaggagc agatgaaagc
    13261 ggcggggaaa gacccggtgc tgctggtgcc gccgcaactg cgtccattgc tggcgcgcta
    13321 tgcaaggttg tttgcgccgg ggctgcatgt gctgtcgtat aacgaagtgc cggatgagct
    13381 ggagttgaag attatggggg cgttgatgta agaatccggc atcggtgcac aattgccgga
    13441 tgcggcgtga acgccttatc cggcctaccg ttaaggcata gatttgtagg catgataaga
    13501 cgcgctagcg tcgcatcagg catctgcgca caaccgccgg atgcggcgtg aacgccttat
    13561 ccggcctacc gttaaggcac agatttgtag gccggataag cgtaagcgca tccggcaccg
    13621 gcgcacaatt ataaaataat acgctccccg gcactaaaat ccgttacgtc ccccgcccaa
    13681 tcgatgggat ataaaccttt cgcgacatcg cgatggaacg ttgagaatgg ccaatcactc
    13741 acctgcttta cccagccatg tttcactgga tttatgtaaa tataatcaac atgatgacga
    13801 taatctttgg tattgcgaat ggcatgctcc caaaaacgcg gttgccacat attttgcaat
    13861 ccacaagcat gggtgaactg ttttttaatc tcccgccagc gagaggaaaa atcatcatcg
    13921 ctttcgggta atgtccagat acagtgcata tgttccggca aaacgaccca ggcgttaatt
    13981 tcaaaaggtc tggcctgctt aactttaata atggcgttac ggagtgtctg gtactgggtg
    14041 gtcaaaagtt ggcttcgacg attccgtaaa tttaccgtga aaaaccatgt tccccccttg
    14101 atgtaataac gacgatattc agacatggcg actccttttt ctcaacgata agaagaaaag
    14161 gaaaagtcga caatgaagtt gtgttatatc gggaaagcag ctcgcaaaaa attcgcagac
    14221 tcgtaggcat gataagacgc ggcagcgtcg catcaggcat cggtgcacta ttgccggatg
    14281 cggcgtgaac gccttatccg tcctacaaaa tcgtttcctc cgtcaaaatc ctgcgcgcgc
    14341 ctaaaaaatg gtcctgccag aaaggttcgg ctaaccgact tacccgaatg gtttccccgg
    14401 tgcgcggtga ctcgataaac tgaccgtctc caagatatac gcccatatgg tcggctatct
    14461 cacgactgtg gatatggaaa aacagcaaat ctccccggcg caggtcgtta ttcgccacaa
    14521 tcgttgcccg atggtagtga tacatctcat tggccgtacg cgggagcttc gcctcgagga
    14581 tcttgttgta ggcataaaaa accaacccgc tacagtcgaa gccttgatcg ggccgcgtgc
    14641 cgccccagac gtaaggctta ccaagctgct gctccaggcg gtgaatcgcc acttcggtga
    14701 tgttgtgtag atggtcgctg cttaagaaat ggttgttctc cgccagcgcc tgccagcgtc
    14761 tgtcactcgc cttgagcggc gcaggaaacc attctggatg ctgttttatc tgctcccgat
    14821 tatgctggcg cagcgccctt ctgcttttgg cattgccttc cacaatatag ctggcctgct
    14881 ttttcagatg ggtttggtac tgttttaata aacgcgcacg gttttgcatc cgctggcggg
    14941 cggcgtagct gaaagagata tgtgatgtgg tggcgctggc gagtaaaggg agcaaaacgc
    15001 ataacgaaaa cacgcctgga tgcagaaatc tgccgtgcaa gaacgaagac atgaaggaca
    15061 taaaccagct gaatgaaggt gagaatttta aaagaccgga ggagtaaacg ggattattaa
    15121 ttgcttattt gcatattact attgcggttt cataatctta ggaaataaga aataaatcta
    15181 caatccatgc gcttttcgga ctcttgaaaa ctgcccctgt gctaaatttg taaaccctgc
    15241 gccgacaggc ttgcgtaaaa ctcttttggt gataatgtga gccttccaaa tccagactat
    15301 tccgatttgg gattcatttt gccgtcctgg cgtgtgtaac cgttttatca aggaataagc
    15361 agtatgcgta aaatcgcatt aattcttgcg atgttgttga tcccgtgcgt ttcgtttgcc
    15421 ggtttgctgg gcagcagtag ctcgaccacg ccagtgagca aagagtataa gcagcagttg
    15481 atgggatccc ctgtctacat ccagatcttc aaggaagaac gtacgctcga tctctacgtc
    15541 aaaatgggcg agcaatatca actgctcgac agctataaaa tctgtaaata ttccggcggc
    15601 ttagggccaa aacagcgtca gggcgatttc aaaagcccgg aagggtttta tagcgtccag
    15661 cgtaatcagt taaaaccaga cagccgttac tacaaagcga ttaatattgg attcccaaac
    15721 gcctatgacc gtgcgcatgg ttatgaaggg aaatacctga tgattcacgg cgattgtgtt
    15781 tccatcggct gctacgcgat gaccaatcag ggtattgatg agatcttcca gttcgttact
    15841 ggtgcgctgg tgtttggtca gccgagcgtg caagtgagta tttacccgtt ccgtatgacc
    15901 gacgccaata tgaagcgcca taaatattcc aactttaagg acttctggga gcaactgaag
    15961 ccaggctacg actactttga gcaaacccgt aagccaccga ccgtttctgt ggtcaatggc
    16021 cgttacgtgg tcagcaagcc gttaagccac gaagttgtgc agccacaact ggcatcaaac
    16081 tacacgctcc ccgaggcaaa ataagcgcca ttcacctggc ataatctttt gccaggtttc
    16141 attgcccgtc agcggctgtg tcgcaatcac cgtgaccaca tcattcggtg tggtctgcga
    16201 gctgaagtcg atttccacat cctgatccag caacgttgcc acgccaaacg gcgcgcggcg
    16261 ggtgatccag tgtaaattag tcgagcaata cgccattaca tagcgcccgt cagaaagcag
    16321 catgttgaaa acgcccttct gccgcagttc atccgccagt gaggcgatat atttaaacac
    16381 tgccgccatg ttgcccggtg tgcgcgggta acgctgcgtt aatttatgca ggagccagca
    16441 aaaggctttt tcgctatcgg tttcaccgac cgggcggaag ttgccggttt ccagtgattt
    16501 gtagcccgtc agttgtccat tatgggcata agtccagtta cgcccccata actcgcgggt
    16561 aaacgggtga gtattttcca gcgccacctc gccccgatta gcctggcgaa tatgagccac
    16621 caccgaacag gattttatcg ggtagtcctg gacaagtttg gcgatggggg aattaaagct
    16681 gggttgcgga tctttaaatg tgcgacagcc tttaccttcg taaaaggtaa tgccccagcc
    16741 atctttatgt ggcccggttc caccaccacg ctgtacaagt ccggtgaaac taaagcagat
    16801 atcggtaggg acgttggcgc tcatcccgag caattcgcac atgggtaacc tccacaacag
    16861 cgggagcgca tggcccccgg cgaagattga ttaaaaagta caaaggttca ggctgttacc
    16921 ctcaccccgg ccctctccct gaaagggaga aaagacctta cttaaccatc tctttttcaa
    16981 ttaactggat caggatatgg atcactttaa tgtggatctc ctgaatgcgg tcggcataac
    17041 caaagtgcgg tacgcgaatt tcgatatccg ctgtgccagc cattttgccg ccgtctttac
    17101 cggtcagggt gatcactttc atccccttct cacgcgccgc cgcgatcgct ttgatcacgt
    17161 ttgcagagtt accggaggtg gagatcccca gcagtacatc gccttcgcga cctaccgctt
    17221 caacgtaacg ggagaaaata tcattgaaac caaaatcgtt accgacgcag gaaatatggc
    17281 tgacgtcaga aatggcaatc gccgggtagc ccggacggtt ttcacggtag cgaccggtca
    17341 actcttcggc aaagtgcata gcgtcgcaat gggagccgcc gttgccgcag gaaagcactt
    17401 tgccaccggc tttaaagctg tctgctaaca ggaccgccgc gcgctgaatg gcgtgaatat
    17461 tggcgtcatc ttttaaaaaa ttagccagcg tttccgccgc ttcgttcagt tcgttacgaa
    17521 taagatcctg gtacatgagg atatccttca gcataaatgt aatagacaaa atgcagtgta
    17581 ccggataccg ccaaaagcga gaagtacggg caggtgctat gaccaggact ttttgacctg
    17641 aagtgcggat aaaaacagca acaatgtgag ctttgttgta attatattgt aaacgtattg
    17701 ctaaatgttt ttacatccac tacaaccata tcatcacaag tggtcagacc tcctacaagt
    17761 aaggggcttt tcgttatgat gattttgagt attctcgcta cggttgtcct gctcggcgcg
    17821 ttgttctatc accgcgtgag cttatttatc agcagtctga ttttgctcgc ctggacagcc
    17881 gcactcggcg ttgctggtct gtggtcggcg tgggtactgg tgcctctggc cattatcctc
    17941 gtgccattta actttgcgcc tatgcgtaag tcgatgattt ccgcgccggt atttcgcggt
    18001 ttccgtaagg tgatgccgcc gatgtcgcgc actgagaaag aagcgattga tgcgggcacc
    18061 acctggtggg aaggcgattt gttccagggc aagccggact ggaaaaagct gcataactat
    18121 ccgcagccgc gcctgaccgc agaagagcaa gcgtttctcg acggcccggt agaagaagcc
    18181 tgcaggatgg cgaatgattt ccagattacc catgagctgg cggatttgcc gccggagctg
    18241 tgggcgtatc ttaaagagca tcgcttcttc gcgatgatca tcaaaaaaga gtacggcggg
    18301 ctggagttct cggcttatgc ccagtctcgc gtgctgcaaa aactctccgg agtgagcggg
    18361 atctggcgat taccgtcggc gtgccaaact cattaggccc gggtgagcta ctgcaacatt
    18421 acggcactga cgagcagaaa gatcactatc tgccgcgtct ggcgcgtggt caggagatcc
    18481 cttgctttgc actgaccagc cccgaagcgg gttccgatgc gggcgcgatt ccggataccg
    18541 ggattgtttg catgggcgag tggcagggcc agcaggtgct ggggatgcga ctgacctgga
    18601 acaaacgcta cattacgctc gcgccgattg cgaccgtgct tggtctggcg tttaaactct
    18661 ccgacccgga aaaactgctg ggtggcgcag aagatttagg tattacctgt gcgctgatcc
    18721 cgaccaccac gccgggcgtg gaaattggtc gtcgccactt cccgctgaac gtgccgttcc
    18781 agaacggacc gacgcgcggt aaagatgtct tcgtaccgat cgattacatt atcggcggac
    18841 ccaaaatggc cgggcaaggc tggcggatgc tggtggagtg cctctcggta ggccgcggta
    18901 tcaccctgcc ttccaactca accggcggcg tgaaatcggt agcgctggca acgggtgcgt
    18961 atgcgcacat ccgccgtcag ttcaaaatct ctattggtaa gatggaaggg attgaagagc
    19021 cgctggcgcg tattgccgga aacgcttatg taatggatgc tgcggcatcg ctgattacct
    19081 acggcattat gctcggtgaa aaacctgccg tgctgtcggc tatcgttaag tatcactgta
    19141 cccaccgtgg gcagcagtcg attattgatg cgatggatat cactggtggc aaaggcatta
    19201 tgctcgggca aagtaacttc ctggcccgtg cttaccaggg cgcaccgatt gccatcaccg
    19261 ttgaaggggc taacattctg acccgcagca tgatgatctt cggacaagga gcgattcgtt
    19321 gccatccgta cgtgctggaa gagatggaag cggcgaagaa caatgacgta aatgcgttcg
    19381 ataaactgct gttcaaacat atcggtcacg tcggtagcaa caaagttcgc agcttctggc
    19441 tgggcctgac gcgcggttta accagcagca cgccaaccgg cgatgccact aaacgctact
    19501 atcagcacct gaaccgcctg agcgccaacc tcgccctgct ttctgatgtc tcgatggcgg
    19561 tgctgggcgg cagcctgaaa cgtcgtgagc gtatctcggc acgtctgggg gatattttaa
    19621 gtcagcttta cctcgcctct gccgttttga agcgttatga cgacgaaggc cgcaacgaag
    19681 ccgacctgcc gctggtgcac tggggcgtac aggatgcgct gtatcaggca gaacaggcga
    19741 tggacgattt actgcaaaac ttcccgaacc gcgtggttgc cgggctgctg aatgtggtga
    19801 tcttcccgac cggacgtcat tatctggcac cttctgacaa gctggatcat aaggtggcga
    19861 agattttaca agtgccgaac gccacccgtt cccgcattgg tcgcggtcag tacctgacgc
    19921 cgagcgagca taacccggtt ggtttactgg aagaggcgct ggtggatgtg attgccgccg
    19981 acccgattca tcagcggatc tgtaaagagc tgggtaaaaa cctgccgttt acccgtctgg
    20041 atgaactggc gcacaacgcg ctggcgaagg ggctgattga taaagatgaa gccgctattc
    20101 tggtgaaagc tgaagaaagc cgtctgcgca gtattaacgt tgatgacttt gatccggaag
    20161 agctggcgac gaagccggta aagttgccgg agaaagtgcg gaaagttgaa gccgcgtaat
    20221 tgaataacgg agccgaaagg ctccgtttct ttatccgcta attatttaaa attaaagcca
    20281 tccggatggt tttccaggct gccggtcaac gccgcgaaca gcaccgtttt accatcaata
    20341 gaaagcgcat cgttcacatt caaccaggtg agtttctctt gcgacgtttt ctcatcaata
    20401 gtcgagaaca gccccgtcat ctgattagat ttctcggacc acatcacagc gatacgttgc
    20461 gagccacagt catgcggttt gcacgcgctc atcacctgat acgtctcatc tcccaatgtc
    20521 acggtttgtg cgggagtata agtaccgcct ttcatcaccc aggcaggcag cttatgccct
    20581 tgcaccatct gattaaacgc cgctttggtg gtttcgccct ttgcaagact gctaatggtt
    20641 aaatcatcct gcgccattgc actggtggcg atgaccagag cggcgactgt cgttattgcc
    20701 ttaaacatca ttcctcccgg gcttatcctg cccatgtgat taactcccta aagcctggcg
    20761 ataacacgat aatttgcaaa tcagatattt ccaatatcag catttgttat caatttattc
    20821 tgcgctaagg caactgcctg ataaggtgtt agagttataa aacccttcgc ataaaggagt
    20881 tgtacaccgt gcctggtttg aagattacgc ttttgcagca accactggtg tggatggatg
    20941 gtcctgccaa cctgcgccat tttgatcgtc aactggaagg tattaccggg cgcgatgtga
    21001 tcgttctacc ggagatgttt accagcggct ttgccatgga agcggcagct tcgtcgctag
    21061 cacaagatga cgtagtgaac tggatgaccg ctaaagcgca gcagtgcaat gcgctgattg
    21121 caggcagtgt tgcattacaa acggagtctg gttcggttaa ccgctttttg ctggttgagc
    21181 cgggcggcac ggtacatttt tatgataagc gtcatctgtt ccgcatggca gatgagcatc
    21241 tgcattataa agcgggcaat gagcgagtga ttgtggaatg gcgcggctgg cgtattttgc
    21301 cgctggtgtg ctacgactta cgttttccgg tgtggtcgcg caatctcaac gattatgacc
    21361 tcgccctgta cgtcgccaac tggcctgctc cgcgctctct gcactggcag gcattgctga
    21421 cagcccgcgc gattgagaat caagcgtatg tggcgggatg caatcgcgtc ggcagcgatg
    21481 gcaacggctg ccattatcgc ggtgacagcc gggtgattaa tccgcaaggc gagattatcg
    21541 ctactgccga cgcgcatcag gcaacgcgca ttgatgcgga gctgtcgatg atggcattgc
    21601 gggaatatag agaaaagttt ccggcatggc gggatgcgga tgagtttagg ttgtggtgag
    21661 tgggggttgt gtttagggct ggggagagtc agggtaagac tacgaaagcc cgctc
//
LOCUS       DAZFLD010000069        21545 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.069, whole genome shotgun sequence.
ACCESSION   DAZFLD010000069 DAZFLD010000000
VERSION     DAZFLD010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21545
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.069"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..2852
                     /locus_tag="ACHKBL_002208"
     CDS             <1..2852
                     /locus_tag="ACHKBL_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YadA family autotransporter adhesin"
                     /protein_id="HFO5819075.1"
                     /translation="GSQLKTTNDAVAANTTNIATNTTNITNLTDAVDSLGDDSLLWNA
                     TAGAFSAKHGTNGTDSKITNLLAGTVSSDSTDAINGSQLYGLADSFTSYLGGGADISD
                     AGVLTGPTYTIGGTDYNNVGDALAAINTSFSTSLGDALLWDATAKGGDGAFSAGRGKD
                     NTASIITNVADGAISSTSSDAINGSQLYDTSKYIADTLGGDAEVNADGTITAPTYAIA
                     GGSYSNVGDALEAIDTTLDDALLWDATANDGNGAFSAAHGKDKTASVITNVANGAISA
                     TSSDAINGSQLYTTNKYIADALGGDAEVNADGSITAPTYTIANAEYNNVGDALDALDD
                     NALLWDATANDGAGAYNASHDGKASIITNVADGNIGEGSTDAINGSQLFNTNMMIQQN
                     SEIINQLAGNTSETYIEDNGAGINYVRTNDNGLAFNDASASGIGATAVGYNSVAEGDS
                     SVAIGQGSYSDVDTGIALGSSSVSSRVIVKGSRDTSVSEEGVVIGYDTTDGELLGALS
                     IGDDGKYRQIINVADGSEAHDAVTVRQLQNAIGAVATTPTKYFHANSTAEDSLAVGED
                     SLAMGAKTIVNGNAGIGIGLNTLVLADAINGIAIGSNARANHANSIAMGNGSQTTRGA
                     QTGYTAYNMDAPQNSVGEFSVGSEDGQRQITNVAAGSADTDAVNVGQLKVTDAQVSQN
                     TQSITNLNTQVTNLDTRVTNIENGIGDIVSTGSTKYFKTNTDGVDANAQGKDSVAIGS
                     GSIAAADNSVALGTGSVANEENTISVGSSTNQRRITNVAAGKNATDAVNVAQLKSSEA
                     GGVRYDTKADGSVDYSNITLGGGNGGTTRISNVSAGVNNNDAVNYAQLKQSVQETKQY
                     TDQRMVEMDNKLSKTESKLSGGIASAMAMTGLPQAYTPGASMASIGGGTYNGESAVAL
                     GVSMVSANGRWVYKLQGSTNSQGEYSAALGAGIQW"
     gene            3264..4919
                     /gene="lldP"
                     /locus_tag="ACHKBL_002209"
     CDS             3264..4919
                     /gene="lldP"
                     /locus_tag="ACHKBL_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015129 - lactate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015727 - lactate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HFO5819076.1"
                     /translation="MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAAS
                     WTVAIALAVALLFYKMPVANALASVIYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDI
                     IRSSILSITPDQRLQMLIVGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLC
                     LIVNTAPVAFGAMGIPILVAGQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWR
                     GIKETWPAVVVAGGSFAIAQYLSSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFR
                     FGDLGASQVDMTLAHTGYTAGQVLRAWTPFLFLTATVTLWSIPPFKALFASGGALYEW
                     VINIPVPYLDKLVARMPPVVSEATAYAAVFKFDWFSATGTAILFAALLSIVWLKMKPS
                     DAISTFGSTLKELALPIYSIGMVLAFAFISNYSGLSSTLALALAHTGHAFTFFSPFLG
                     WLGVFLTGSDTSSNALFAALQATAAQQIGVSDLLLVAANTTGGVTGKMISPQSIAIAC
                     AAVGLVGKESDLFRFTVKHSLIFTCMVGVITTLQAYVLTWMIP"
     gene            4919..5695
                     /gene="lldR"
                     /locus_tag="ACHKBL_002210"
     CDS             4919..5695
                     /gene="lldR"
                     /locus_tag="ACHKBL_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LldR"
                     /protein_id="HFO5819077.1"
                     /translation="MIVLPRRLSDEVADRVRALIDEKNLEAGMKLPAERQLAMQLGVS
                     RNSLREALAKLVSEGVLLSRRGGGTFIRWRHDTWSEQNIVQPLKTLMADDPDYSFDIL
                     EARYAIEASTAWHAAMRATPGEKEKIQLCFEATLSEDPDLASQADVRFHLAIAEASHN
                     IVLLQTMRGFFDVLQSSVKHSRQRMYLVPPVFSQLTEQHQAVIDAIFAGDADGARKAM
                     MAHLSFVHTTMKRFDEDQARHARITRLPGDHNEHSREKNA"
     gene            5692..6882
                     /gene="lldD"
                     /locus_tag="ACHKBL_002211"
     CDS             5692..6882
                     /gene="lldD"
                     /locus_tag="ACHKBL_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent L-lactate dehydrogenase"
                     /protein_id="HFO5819078.1"
                     /translation="MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSE
                     VALRQRILKNMSDLSLETTLFNEKLSMPVALGPVGLCGMYARRGEVQAAKAADAHGIP
                     FTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPT
                     PGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLE
                     DYIGWLGNNFDPSISWKDLEWIREFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHG
                     GRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTVLLGRAFLY
                     ALATAGQAGVANLLNLIEKEMKVAMTLTGAKSISEITQDSLVQGLGKELPAALAPMAK
                     GNAA"
     gene            6955..7428
                     /gene="trmL"
                     /locus_tag="ACHKBL_002212"
     CDS             6955..7428
                     /gene="trmL"
                     /locus_tag="ACHKBL_002212"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HFO5819079.1"
                     /translation="MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRL
                     RRAGLDYHEFTAVTRHHDYRAFLEAENPQRLFALTTKGTPAHSAVSYQDGDYLMFGPE
                     TRGLPASILDALPTEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGAVLRD"
     gene            complement(7481..8302)
                     /gene="cysE"
                     /locus_tag="ACHKBL_002213"
     CDS             complement(7481..8302)
                     /gene="cysE"
                     /locus_tag="ACHKBL_002213"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HFO5819080.1"
                     /translation="MSCEELEIVWNNIKAEARTLADCEPMLASFYHATLLKHENLGSA
                     LSYMLANKLSSPIMPAIAIREVVEEAYAADPEMIASAACDIQAVRTRDPAVDKYSTPL
                     LYLKGFHALQAYRIGHWLWNQGRRALAIFLQNQVSVTFQVDIHPAAKIGRGIMLDHAT
                     GIVVGETAVIENDVSILQSVTLGGTGKSGGDRHPKIREGVMIGAGAKILGNIEVGRGA
                     KIGAGSVVLQPVPPHTTAAGVPARIVGKPDSDKPSMDMDQHFNGINHTFEYGDGI"
     gene            complement(8382..9401)
                     /gene="gpsA"
                     /locus_tag="ACHKBL_002214"
     CDS             complement(8382..9401)
                     /gene="gpsA"
                     /locus_tag="ACHKBL_002214"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFO5819081.1"
                     /translation="MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATL
                     ERDRCNAAFLPDVPFPDTLHLESDLATALAASRNILVVVPSHVFGEVLRQIKPLMRPD
                     ARLVWATKGLEAETGRLLQDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTD
                     QTFADDLQQLLHCGKSFRVYSNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTAL
                     ITRGLAEMSRLGAALGADPATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSA
                     QEKIGQVVEGYRNTKEVRELAHRFGVEMPITEEIYQVLYCGKNAREAALTLLGRARKD
                     ERSSH"
     gene            complement(9401..9868)
                     /gene="secB"
                     /locus_tag="ACHKBL_002215"
     CDS             complement(9401..9868)
                     /gene="secB"
                     /locus_tag="ACHKBL_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015369180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HFO5819082.1"
                     /translation="MSEQNNTEMTFQIQRIYTKDISFEAPNAPHVFQKDWQPEVKLDL
                     DTASSQLADDVYEVVLRVTVTASLGEETAFLCEVQQGGIFSIAGIEGTQMAHCLGAYC
                     PNILFPYARECITSMVSRGTFPQLNLAPVNFDALFMNYLQQQAGEGTEEHQDA"
     gene            complement(9931..10182)
                     /gene="grxC"
                     /locus_tag="ACHKBL_002216"
     CDS             complement(9931..10182)
                     /gene="grxC"
                     /locus_tag="ACHKBL_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HFO5819083.1"
                     /translation="MANVEIYTKETCPYCHRAKALLSSKGVSFQELPIDGNAAKREEM
                     IKRSGRTTVPQIFIDAQHIGGCDDLYALDARGGLDPLLK"
     gene            complement(10324..10755)
                     /locus_tag="ACHKBL_002217"
     CDS             complement(10324..10755)
                     /locus_tag="ACHKBL_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFO5819084.1"
                     /translation="MQEIMQFVGRHPILSIAWIALLVAVIVTTFKSLTSKVKVITRGE
                     ATRLINKEDAVVVDLRQRDDFRKGHIAGSINLLPSEIKANNVGELEKHKDKPVIVVDG
                     SGMQCQEPANALTKAGFAQVFVLKEGVAGWAGENLPLVRGK"
     gene            11000..12544
                     /gene="gpmM"
                     /locus_tag="ACHKBL_002218"
                     /gene_synonym="pgmI"
     CDS             11000..12544
                     /gene="gpmM"
                     /locus_tag="ACHKBL_002218"
                     /gene_synonym="pgmI"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HFO5819085.1"
                     /translation="MSVSKKPMVLVILDGYGYREEQQDNAIFSAKTPVMDALWANRPH
                     TLIDASGLEVGLPDRQMGNSEVGHVNLGAGRIVYQDLTRLDVEIKDRAFFANPVLTGA
                     VDKAKNAGKAVHIMGLLSAGGVHSHEDHIMAMVELAAERGAEKIYLHAFLDGRDTPPR
                     SAESSLKKFEEKFAALGKGRVASIIGRYYAMDRDNRWDRVEKAYDLLTLAQGEFQADT
                     AVAGLQAAYARDENDEFVKATVIRAEGQPDAAMEDGDALIFMNFRADRAREITRAFVN
                     ADFDGFARKKVVNVDFVMLTEYAADIKTAVAYPPASLVNTFGEWMAKNDKTQLRISET
                     EKYAHVTFFFNGGVEESFKGEDRILINSPKVATYDLQPEMSSAELTEKLVAAIKSGKY
                     DTIICNYPNGDMVGHTGVMEAAVKAVEALDHCVEEVAKAVESVGGQLLITADHGNAEQ
                     MRDPATGQAHTAHTNLPVPLIYVGDKNVKAVEGGKLSDIAPTMLSLMGMEIPQEMTGK
                     PLFIVE"
     gene            12578..13837
                     /gene="envC"
                     /locus_tag="ACHKBL_002219"
     CDS             12578..13837
                     /gene="envC"
                     /locus_tag="ACHKBL_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418070.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HFO5819086.1"
                     /translation="MTRAVKPRRFAIRPIIYASVLSAGVLLCAFSAHADERDQLKSIQ
                     ADIAAKERAVRQKQQQRASLLAQLKKQEEAISEATRKLRETQNTLNQLNKQIDEMNAS
                     IAKLEQQKAAQERSLAAQLDAAFRQGEHTGIQLILSGEESQRGQRLQAYFGYLNQARQ
                     ETIAQLKQTREEVAMQRAELEEKQSEQQTLLYEQRAQQAKLTQALNERKKTLAGLESS
                     IQQGQQQLSELRANESRLRNSIARAEAAAKARAEREAREAQAVRDRQKEATRKGTTYK
                     PTESEKSLMSRTGGLGAPRGQAFWPVRGPTLHRYGEQLQGELRWKGMVIGASEGTEVK
                     AIADGRVILADWLQGYGLVVVVEHGKGDMSLYGYNQSALVSVGSQVRAGQPIALVGSS
                     GGQGRPSLYFEIRRQGQAVNPQPWLGR"
     gene            13841..14800
                     /locus_tag="ACHKBL_002220"
     CDS             13841..14800
                     /locus_tag="ACHKBL_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HFO5819087.1"
                     /translation="MFPFRRNVLAFAALLALSSPVIAGKLAIVIDDFGYRPHNENQVL
                     AMPSAISVAVLPDSPHAREMATKAHNSGHEVLIHLPMAPLSKQPLEKNTLRPEMSSDE
                     IERIIRSAVNNVPYAVGINNHMGSKMTSNLFGMQKVMQALERYNLYFLDSVTIGNTQA
                     MRAAQGTGVKVIKRKVFLDDSQNEADIRVQFNRAIDLARRNGSTIAIGHPHPSTVRVL
                     QQMVYNLPPDITLVKASSLLNEPQVDTSTPPKNTVPDTPRNPFRGVKLCKPKKPIEPV
                     YANRFFEVLSESISQSTLIVYFQHQWQGWGKQPEAAKLNASVN"
     gene            complement(14787..15821)
                     /gene="waaH"
                     /locus_tag="ACHKBL_002221"
     CDS             complement(14787..15821)
                     /gene="waaH"
                     /locus_tag="ACHKBL_002221"
                     /EC_number="2.4.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronate:LPS(HepIII) glycosyltransferase"
                     /protein_id="HFO5819088.1"
                     /translation="MMNSTNKLSVIIPLYNAGDDFRTCMESLITQTWTALEIIIINDG
                     STDNSVEIAKHYAENYPHVRLLHQANAGASVARNRGIEVATGKYVAFVDADDEVYPTM
                     YETLMTMALEDDLDVAQCNADWCFRETGETWQSIPTDRLRSTGVLTGPDWLRMGLSSR
                     RWTHVVWMGVYRRDVIVKNNIKFIAGLHHQDIVWTTEFMFNALRARYTEQSLYKYYLH
                     NTSVSRLNRQGNKNLNYQRHYIKITRLLEKLNRNYADKITIYPEFHQQITYEALRVCH
                     AVRKEPDIITRQRMIAEIFTSGMYKRLITNVRSVKVGYQALLWSFRLWQWRDKTRSHH
                     RITRSAFNLR"
     gene            complement(16060..17085)
                     /gene="tdh"
                     /locus_tag="ACHKBL_002222"
     CDS             complement(16060..17085)
                     /gene="tdh"
                     /locus_tag="ACHKBL_002222"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HFO5819089.1"
                     /translation="MKALSKLKAEEGIWMTDVPVPELGHNDLLIKIRKTAICGTDVHI
                     YNWDEWSQKTIPVPMVVGHEYVGEVVGIGQEVKGFKIGDRVSGEGHITCGHCRNCRGG
                     RTHLCRNTIGVGVNRPGCFAEYLVIPAFNAFKIPDNISDDLASIFDPFGNAVHTALSF
                     DLVGEDVLVSGAGPIGIMAAAVAKHVGARYVVITDVNEYRLELARKMGITRAVNVAKE
                     NLNDVMAELGMTEGFDVGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWTKV
                     IFKGLFIKGIYGREMFETWYKMAALIQSGLDLSPIITHRFSIDDFQKGFDAMRSGQSG
                     KVILSWD"
     gene            complement(17095..18291)
                     /gene="kbl"
                     /locus_tag="ACHKBL_002223"
     CDS             complement(17095..18291)
                     /gene="kbl"
                     /locus_tag="ACHKBL_002223"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HFO5819090.1"
                     /translation="MRGEFYQQLTNDLETARAEGLFKEERIITSAQQADITVADGSHV
                     INFCANNYLGLANHPDLIAAAKAGMDSHGFGMASVRFICGTQDSHKELEQKLAAFLGM
                     EDAILYSSCFDANGGLFETLLGAEDAIISDALNHASIIDGVRLCKAKRYRYANNDMQE
                     LEARLKEAREAGARHVLIATDGVFSMDGVIANLKGVCDLADKYDALVMVDDSHAVGFV
                     GENGRGSHEYCDVMGRVDIITGTLGKALGGASGGYTAARKEVVEWLRQRSRPYLFSNS
                     LAPAIVAASIKVLEMVEAGSELRDRLWANARQFREQMSAAGFTLAGADHAIIPVMLGD
                     AVVAQKFARELQKEGIYVTGFFYPVVPKGQARIRTQMSAAHTPEQITRAVEAFTRIGK
                     QLGVIA"
     gene            18505..19437
                     /gene="rfaD"
                     /locus_tag="ACHKBL_002224"
     CDS             18505..19437
                     /gene="rfaD"
                     /locus_tag="ACHKBL_002224"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HFO5819091.1"
                     /translation="MIIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTKFVNLVD
                     LDIADYMDKEDFLIQIMAGEEFGDVEAIFHEGACSSTTEWDGKYMMDNNYQYSKELLH
                     YCLEREIPFLYASSAATYGGRTSDFIESREYEKPLNVYGYSKFLFDEYVRQILPEANS
                     QIVGFRYFNVYGPREGHKGSMASVAFHLNTQLNNGESPKLFEGSENFKRDFVYVGDVA
                     DVNLWFLENGVSGIFNLGTGRAESFQAVADATLAYHKKGQIEYIPFPDKLKGRYQAFT
                     QADLTNLRAAGYDKPFKTVAEGVTEYMAWLNRDA"
     gene            19447..20493
                     /gene="rfaF"
                     /locus_tag="ACHKBL_002225"
     CDS             19447..20493
                     /gene="rfaF"
                     /locus_tag="ACHKBL_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose--LPS heptosyltransferase RfaF"
                     /protein_id="HFO5819092.1"
                     /translation="MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPL
                     LSRMPEVNEAIPMPLGHGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVPFFAGI
                     PHRTGWRGEMRYGLLNDVRVLDKEAWPLMVERYVALAYDKGIMRTAQDLPQPLLWPQL
                     QVSEGEKSYTCNQFSLSSERPMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVL
                     FGSAKDHEAGNEILAALNTEQQAWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVA
                     AALNRPLVALYGPSSPDFTPPLSHKARVIRLITGYHKVRKGDAAEGYHQSLIDITPQR
                     VLEELNALLLQEEA"
     gene            20497..21477
                     /gene="rfaC"
                     /locus_tag="ACHKBL_002226"
     CDS             20497..21477
                     /gene="rfaC"
                     /locus_tag="ACHKBL_002226"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HFO5819093.1"
                     /translation="MRVLIVKTSSMGDVLHTLPALTDAQQAIPGIKFDWVVEEGFAQI
                     PSWHAAVERVIPVAIRRWRKAWFSAPIKAERKAFREALQAENYDAVIDAQGLVKSAAL
                     VTRLAHGVKHGMDWQTAREPLASLFYNRKHHIAKQQHAVERTRELFAKSLGYSKPQTQ
                     GDYAIAQHFLTNLPTDAGEYAVFLHATTRDDKHWPEEHWRELIGLLADSGIRIKLPWG
                     APHEEERAKRLAEGFAYVEVLPKMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNI
                     TVYGPTDPGLIGGYGKNQMVCRAPGNELSQLTANAVKQFIEENAEKAAMI"
BASE COUNT         5248 a         5708 c         5633 g         4956 t
ORIGIN      
        1 acggctctca gctcaaaacc actaacgatg ccgtggcagc caacaccacc aatatcgcca
       61 cgaacaccac caacatcacc aacctgactg acgctgttga cagtctcggt gacgattccc
      121 tgctgtggaa cgctacggcg ggtgcattca gtgccaaaca cggcaccaac ggtactgaca
      181 gcaaaatcac caacttactg gcaggcactg tatcctctga tagcactgac gccattaatg
      241 gctcacaact ttatggttta gctgattcat ttacgtcata ccttggcggt ggtgctgata
      301 tcagcgatgc gggtgtatta accgggccaa cctacactat tggtggtact gactacaata
      361 acgtcggtga tgctctggca gccattaaca cgtcatttag cacatcactc ggtgacgccc
      421 tactctggga tgcgaccgcc aagggcggtg acggtgcgtt tagcgccggt cgcggaaaag
      481 ataacacggc cagcatcatt actaatgttg cagacggtgc aatttcctct accagcagcg
      541 acgctattaa cggctcacag ctctatgaca ccagtaagta cattgccgat actctgggtg
      601 gtgacgcaga agtcaatgct gacggcacaa tcaccgcacc gacttatgct atcgctggcg
      661 gcagttacag caacgtcggt gacgcgctgg aagcaatcga taccacgctc gatgacgctc
      721 tgttgtggga tgccacagcc aatgatggta atggtgcatt tagcgccgct cacggaaaag
      781 ataaaacagc cagtgtaatc actaacgtcg ctaacggtgc aatctctgcc accagcagcg
      841 atgccattaa cggttcacaa ctgtatacca ccaacaagta cattgccgat gccctgggtg
      901 gtgacgcaga agttaacgct gatggttcta tcactgcgcc gacttacacc attgcaaatg
      961 ccgagtacaa caacgtcggt gacgccctgg atgcgctcga cgataacgct ctgctgtggg
     1021 atgcaacggc caatgacggc gcaggtgcct acaacgccag ccatgacggc aaggccagca
     1081 tcatcacaaa tgttgctgat ggtaacattg gcgaaggcag taccgacgcc atcaacggtt
     1141 ctcagctgtt taacaccaat atgatgatcc agcagaacag cgaaatcatc aatcagcttg
     1201 ccggtaacac cagcgaaacc tacatcgaag ataacggtgc gggtattaac tatgtacgta
     1261 ccaacgacaa cggcttagcg ttcaacgatg ccagcgcttc aggtattggc gctacggctg
     1321 tgggttataa ctctgttgcc gaaggcgata gcagcgtagc catcggtcag ggcagctaca
     1381 gcgacgttga taccggtatt gccctgggta gcagctccgt ttccagccgt gtaatagtta
     1441 aaggttctcg tgacaccagc gtgtcggaag aaggtgttgt gattggttat gacaccacgg
     1501 atggcgaact acttggtgcg ctgtctattg gtgatgacgg taaatatcgt caaatcatca
     1561 acgtagccga tggttccgaa gcccatgacg ccgttacggt tcgccagttg caaaatgcca
     1621 ttggtgcagt cgctaccacg ccgaccaaat acttccacgc caactcaacg gctgaagact
     1681 cactggctgt cggggaagac tcgctggcga tgggcgcgaa aactatcgtc aatggtaatg
     1741 cgggtattgg tatcggcctg aatactctgg ttctggcaga tgccatcaac ggtattgcta
     1801 tcggttctaa cgcacgcgcc aaccatgcaa acagtattgc aatgggtaat ggttctcaga
     1861 ccactcgcgg cgctcagact ggctacaccg cctacaacat ggatgcaccg cagaactctg
     1921 tgggtgaatt ctccgtaggt agcgaagacg gtcaacgtca gatcaccaac gtcgcagcgg
     1981 ggtcggcgga taccgatgcg gttaacgtgg gtcagttgaa agtaacggac gcgcaggttt
     2041 cccagaatac ccagagcatt actaacctga atactcaggt aacgaatctg gatacccgcg
     2101 tcaccaatat cgaaaacggt attggcgata tcgtaagcac cggtagcacc aagtacttca
     2161 agaccaacac cgatggcgta gatgctaacg cgcagggtaa agacagtgtt gcgattggct
     2221 ctggctctat tgctgccgct gataacagcg ttgcgctggg tactggctct gtggcaaacg
     2281 aagaaaatac gatctccgta ggttcttcca ctaaccaacg ccgtatcacc aacgttgccg
     2341 ctggtaaaaa tgctactgac gcggttaacg ttgcgcagtt gaagtcttcc gaagcaggcg
     2401 gcgtgcggta tgacaccaaa gctgacggtt ctgttgatta cagcaacatc actctcggcg
     2461 gtggtaatgg cggaacgact cgcatcagca acgtttccgc tggcgttaac aacaacgacg
     2521 cggtgaacta cgcgcagttg aagcaaagcg tgcaggaaac gaagcaatac accgatcagc
     2581 gcatggttga gatggataac aaactgtcca aaaccgaaag caagttgagc ggtggtatcg
     2641 cttctgcaat ggcgatgacc ggtctgccgc aggcttacac gccaggtgcc agcatggcct
     2701 ctattggtgg cggtacttac aacggtgaat cggctgttgc tttaggtgtg tcgatggtga
     2761 gcgccaatgg tcgttgggtc tacaaattac aaggtagtac caatagccag ggtgaatact
     2821 ccgccgcgct cggtgccggt attcagtggt aatcatccat taacgaatgg gtaaacgcca
     2881 cattgcctga tgcgctacgc ttatcaggcc tacggggtga ttcagcacta ccctgtaggc
     2941 cggataaggc gttcacgccg catccggcaa gataaggcgc accagatcaa caatctgagg
     3001 gcaattctct gatgggaatt gcccttttct tcattacctc tctcccagaa ttggccctac
     3061 caattcttca ttgatctgac ctctggttca caattcccca attaaaactc acatcaccgt
     3121 tgccaataca taacatctag ttaactattc attgtcatta tccctacaca acacaattgg
     3181 cagtgccact tttacacaac gtgtgacaag gagatgagca acagactcat tacacggcgt
     3241 gtgtggactg caggagacct gcaatgaatc tctggcaaca aaactacgat cccgccggga
     3301 atatctggct ttccagcctg atagcatcgc ttcccatcct gtttttcttt tttgcgctga
     3361 ttaagctcaa actgaaagga tacgtcgccg cctcatggac ggtggcaatc gcccttgccg
     3421 tggctttgct gttctataaa atgccggtcg ctaacgcgct ggcctcggtg atttatggct
     3481 tcttctacgg tttgtggccc atcgcgtgga tcattattgc ggcagtgttc gtctataaga
     3541 tctcggtgaa aaccgggcag tttgacatca tccgctcgtc tattctttcg ataacccctg
     3601 accagcgcct gcaaatgctg atcgtcggtt tctgtttcgg cgctttcctt gaaggagccg
     3661 caggctttgg cgctccggta gcaattaccg ccgcattgct ggtcggtctg ggctttaaac
     3721 cactctacgc cgccggactg tgcctgattg ttaacaccgc gccagtggca tttggtgcga
     3781 tgggtattcc gattctggtc gccgggcagg taacaggcat cgacagcttt gagattggtc
     3841 agatggtggg gcgtcagttg ccgtttatga ccattatcgt gctgttctgg atcatggcga
     3901 ttatggacgg ttggcgcgga atcaaagaaa cgtggcctgc ggtcgtggtg gcaggtggtt
     3961 cgtttgccat tgctcaatac ctcagctcta acttcattgg gccggaactg ccagacatta
     4021 tctcttcgct ggtatcactg ctctgcctga cgctgttcct caaacgctgg caaccagtgc
     4081 gcgtattccg tttcggtgac ttgggggcgt cacaggttga tatgacgctg gctcacaccg
     4141 gttacaccgc aggtcaggtg cttcgcgcct ggacaccgtt cctgttcctg accgccaccg
     4201 taacgctgtg gagtatcccg ccgtttaaag ccctgttcgc ttcaggcggt gcgctgtatg
     4261 agtgggtgat caacattccg gtgccgtacc tcgataaact ggttgcccgt atgccgccag
     4321 tggtcagcga agcgacagcg tatgccgccg tgtttaagtt tgactggttc tctgccaccg
     4381 gtaccgccat tctttttgct gccctgctct caattgtctg gctgaagatg aaaccatctg
     4441 acgctatcag caccttcggc agcacgctga aagaactggc tctgcctatc tactccatcg
     4501 gtatggtgct ggcgttcgcc tttatctcga actattccgg actatcatca acgctggcgc
     4561 tggcactggc gcacaccggc catgcattca ccttcttctc gccgttcctc ggctggcttg
     4621 gtgtcttcct gaccggatcg gatacctcat ctaacgccct gttcgccgcc ctgcaagcca
     4681 ctgcagcaca acaaattggc gtttctgacc tgttgttggt tgccgccaac accaccggtg
     4741 gtgtcaccgg taagatgatc tctccgcaat ctatcgctat cgcctgtgcg gcggtaggcc
     4801 tggtgggcaa agagtctgat ttgttccgct ttactgtcaa acacagcctg atcttcacct
     4861 gtatggtggg cgtgatcacc acgcttcagg cttatgtctt aacgtggatg attccttaat
     4921 gattgtttta cccagacgcc tgtcagacga ggttgccgat cgtgtgcggg cgctgattga
     4981 tgaaaaaaac ctggaagcgg gcatgaagtt gcccgctgaa cgtcaactgg cgatgcaact
     5041 cggcgtgtcg agaaattcac tgcgcgaggc gctggcgaaa ctggtcagtg aaggtgtgct
     5101 gctcagtcga cgtggcggcg ggacgtttat tcgctggcgt catgatacat ggtcggagca
     5161 aaacatcgtc cagccgctga aaacgctgat ggccgatgat ccggattaca gtttcgatat
     5221 tctggaagcc cgctacgcca ttgaagccag caccgcgtgg catgcggcaa tgcgcgccac
     5281 accaggcgaa aaagagaaaa ttcagctttg ctttgaagca acgctaagtg aagacccgga
     5341 tctcgcctca caagcggacg ttcgttttca tctggcgatt gccgaagcct cacataacat
     5401 cgtgctgctg caaaccatgc gcggtttctt cgatgtcctg caatcctctg tgaagcatag
     5461 ccgccagcgg atgtatctgg tacccccggt tttttcacag ctgaccgaac aacatcaggc
     5521 agtcattgac gccatttttg ccggtgatgc tgacggggcg cgtaaagcaa tgatggcgca
     5581 ccttagcttt gttcacacca ccatgaaacg attcgatgaa gatcaagctc gccatgcacg
     5641 cattacccgc ctgcctggtg accataatga gcattcgagg gagaaaaacg catgattatt
     5701 tccgcagcca gcgattatcg cgccgcagca caacgcattc tgccgccgtt cctgttccac
     5761 tatatggatg ggggtgcata ttctgaatac acgctgcgcc gcaacgtgga agatttgtca
     5821 gaagtggcgc tgcgccagcg tattctgaaa aacatgtctg acttaagcct ggaaacgacg
     5881 ctgtttaatg agaaattgtc gatgccggtg gcgctaggtc cggtaggttt gtgtggcatg
     5941 tatgcgcgac gcggcgaagt tcaggctgcc aaagccgcgg atgcgcatgg cattccgttt
     6001 actctctcga cggtttccgt ttgcccgatt gaagaagtgg ctccagccat taaacgtccg
     6061 atgtggttcc agctttatgt gctgcgcgat cgcggcttta tgcgtaacgc actggagcga
     6121 gcaaaagcgg caggctgctc gacactggtt ttcaccgtgg atatgccgac accgggcgcg
     6181 cgttaccgtg atgcacactc gggtatgagc ggcccaaatg cggcaatgcg ccgttacttg
     6241 caggcggtga cgcatccgca atgggcgtgg gatgtgggcc tgaacggtcg tccacatgat
     6301 ttaggtaata tctcggctta cctcggcaaa ccgaccggac tggaagatta catcggctgg
     6361 ctggggaata acttcgatcc gtccatctca tggaaagacc ttgagtggat ccgcgaattc
     6421 tgggatggtc cgatggtgat caaagggatc ctcgatccgg aagatgcgcg cgatgcagta
     6481 cgttttggtg ctgatgggat tgtggtttct aaccacggtg gccgccagct ggacggtgta
     6541 ctctcttccg ctcgtgcatt gcccgctatt gcggatgcgg tgaaaggtga tattgccatt
     6601 ctggcagata gcggaatacg taacgggctt gatgtcgtgc gtatgattgc gctcggtgcc
     6661 gacaccgtac tgctgggtcg tgctttcctg tatgcgctgg caacagcggg ccaggcgggc
     6721 gtggcaaatc tgctgaatct gatcgaaaaa gagatgaaag tggcaatgac gctgactggc
     6781 gcgaaatcga ttagcgaaat tacgcaagat tcgctggtgc aggggctggg taaagagttg
     6841 ccggcggcac tggctccgat ggcgaaaggg aatgcggcat agttgggggt gaggggaaaa
     6901 ggccgtacca atcaccaatt ttctgctatc ctgcccccgc actaaggggg cagtatgcta
     6961 aacatcgtac tttacgaacc agaaattccg ccaaatactg gcaacatcat ccgtctttgc
     7021 gctaataccg gctttcgtct gcatatcatc gaaccaatgg gatttgcctg ggacgataag
     7081 cgcctgcgcc gcgcggggct ggactatcac gagtttaccg ccgttacgcg tcatcatgac
     7141 tatcgcgcgt tcctcgaagc agaaaatccc cagcgcctgt ttgccctcac cacgaaaggt
     7201 acgcctgctc acagcgccgt aagctatcag gatggcgatt atctgatgtt cggcccggaa
     7261 acacgcggcc tgccagcgag cattcttgat gccctgccca ctgaacaaaa aattcgtatt
     7321 ccaatggtgc cggacagccg cagcatgaac ctgtctaatg cggtgtcggt cgtggtgtat
     7381 gaagcctggc ggcagttggg gtatccggga gcggtgttga gagattagtt actgtaggcc
     7441 ggatagatga ttacatcgca tccggcacga tcacaggaca tcagatccca tcaccatact
     7501 caaacgtatg gttaatgccg ttgaaatgct gatccatatc cattgacggt ttatcgctat
     7561 ccggcttgcc gacaatgcgt gccggaacgc cagcggcagt ggtgtgcggc ggcaccggtt
     7621 gcagcaccac ggaacctgcg ccaatcttcg cgccgcgccc aacttcaata ttaccgagaa
     7681 ttttcgcgcc cgcgccaatc atcacgcctt cacgaatttt cgggtgacgg tcaccaccag
     7741 atttacccgt accgccaagc gtcacagatt gcagaatcga tacgtcgttt tcaatcaccg
     7801 ccgtttcacc cacaacgatg cccgtcgcgt ggtcgagcat gataccgcga ccaatttttg
     7861 ctgccgggtg aatatcaacc tggaacgtta cagagacctg gttttgtaga aaaatagcca
     7921 gcgcgcgacg gccctgattc cacaaccagt gaccgatgcg atacgcctgc aaagcatgaa
     7981 aacccttcag gtatagcaac ggggttgagt atttatccac cgccgggtca cgggtacgca
     8041 ccgcctgaat atcacaggcc gcagaggcga tcatttccgg gtcggcggcg taggcttctt
     8101 ccaccacttc acggatagca atagccggca taatgggcga tgacagcttg ttcgccagca
     8161 tgtagctcag tgcactgcca aggttttcgt gcttgagtag cgtcgcgtgg taaaaactgg
     8221 ccagcattgg ctcacagtct gccagcgttc tggcttcagc tttaatattg ttccagacaa
     8281 tttccagttc ttcacacgac attgcttact ccacacgata agataatgac cggcccgttc
     8341 tgcgcgggcc gggtcatagt ggtaacaaag gttccctggg tttagtggct gctgcgctcg
     8401 tccttgcgtg cacgacctag taaagtcaat gctgcctcgc gcgcgttttt tccgcaatat
     8461 aatacttgat aaatttcctc ggttattggc atttcaacgc cgaagcgatg cgccagttcg
     8521 cggacttctt tcgtattgcg gtagccttcc accacttgac caattttctc ctgcgcgctt
     8581 tgtacatcca tgccctgacc gagcatcatg ccaaaacggc ggttacgcga ctggttgtcg
     8641 gtacaggtca gcaccagatc gcccagcccc gccatgccca taaaggtggc aggatcggca
     8701 cccagcgccg cgccaagacg cgacatctca gccagcccac gggtgatcag cgccgtacgc
     8761 gcattcgcgc caaaaccgat accgtcggac atccccgcgc caatggcaat gacgtttttc
     8821 accgcgccgc caagctgcac accgatgaaa tccgggttgc tgtaaacgcg gaaacttttg
     8881 ccacagtgca gcaattgctg gaggtcatcg gcaaaggtct ggtccgtcga ggccagcgaa
     8941 atggctgtcg gtaaacctgc cgccagttct ttcgcaaacg ttgggccaga gataaccgcc
     9001 agcggaattt gatcgcctaa ggcctcacgc gctacgtcct gtaacagacg tccggtttct
     9061 gcttccagcc ctttggtcgc ccacaccaga cgcgcatcag gacgcatcag cggtttaatc
     9121 tggcgcagca cttcaccaaa gacatggctg ggtacgacga cgagaatatt acggctggct
     9181 gccagcgcag tggcgagatc gctttcaaga tggagcgtat cgggaaaagg cacatcgggg
     9241 agaaacgcgg cgttacagcg gtcgcgttca agcgttgcga tatgttcagg gtcatggccc
     9301 cagaggacaa cctcgtggcc atttcttgcc agggtaatgg caagagcggt gccgtacgag
     9361 ccggcaccga tcacagtcat tgaagcatta cgttggttca tcaggcatcc tgatgttctt
     9421 cagtaccttc gccagcctgc tgctgcaaat agttcatgaa cagcgcatcg aagttaaccg
     9481 gcgcaaggtt cagttgcggg aatgtaccgc gggataccat gctggtgatg cactcacgag
     9541 catacgggaa cagaatgttc gggcagtatg cacccaggca atgtgccatc tgggtgcctt
     9601 cgatacccgc gatggagaaa ataccgccct gctgaacttc acacaggaac gcggtttctt
     9661 cgcccaggga ggccgttacg gtaacacgca gtaccacttc gtaaacgtca tctgccagtt
     9721 gagaagatgc cgtatccaga tcaagtttaa cttctggctg ccaatctttc tggaaaacgt
     9781 gcggcgcgtt cggcgcttcg aaagaaatat ccttggtata aatacgttgg atctggaaag
     9841 tcatttcggt gttgttttgt tctgacatgt gtagaaaacc cttaagtgtt gtccttaaat
     9901 actgcgcaat gtaatgccaa cgttcacacg ttatttcagc aggggatcca gtccaccacg
     9961 tgcatccaat gcatacaagt catcacagcc gccaatgtgc tgtgcgtcaa taaaaatctg
    10021 gggaaccgtg gtgcgaccgc tgcgtttgat catctcttca cgcttagcgg cgttgccatc
    10081 gatcggcaac tcctgaaaac tcacgccctt gctgctcagc agtgctttcg cacgatggca
    10141 atacgggcag gtttctttgg tatagatttc aacattggcc ataacttagc tcctgttttt
    10201 ttaccctgaa atttcatgtt gcagggcggc tgcaaaatgg agtgtcccca gaagcttata
    10261 acaataagtc gctgggggcc taattgcagc taacggcctg catcatgaaa gacgacaggt
    10321 aaattatttg ccgcgcacca gaggtaagtt ttcgccagcc cagccagcaa cgccctcttt
    10381 cagcacgaat acttgcgcaa aaccagcttt cgtcagcgcg tttgcaggct cctggcactg
    10441 catgccagaa ccgtctacca cgataaccgg tttgtctttg tgcttctcaa gctcaccaac
    10501 attgttggct ttgatttcgc tcggcaacag gttgatagaa cctgcgatat ggcctttacg
    10561 gaagtcatca cgctgacgta aatccacaac cacagcatct tctttgttga tcagacgcgt
    10621 agcttcacca cgagtaatca ccttcacttt cgaggtcagg ctcttaaacg tagtcacaat
    10681 aaccgccacc agtaacgcga tccaggcgat actcagtatg ggatgacggc caacaaattg
    10741 cataatttct tgcatggggg gtaacaactc ccgacgtagt gattaaaaaa accaggaaag
    10801 gagtatacct gcgcggtgca gcaaatacag ccagcgcgtt aactggaatg caattttgcg
    10861 gggcgcgacg aaaaaaaagc gcaaatctgg catcggcttg ctgcgagcgg tcgtatattt
    10921 tgatcttcag aggctatttt atcgattcag ctgtagtaaa attacgcaaa ttttgactct
    10981 tgagtatgag gttgtcgcaa tgtcggtttc taaaaaacct atggtactgg tgattctgga
    11041 tggctatggc tatcgtgaag aacagcagga taacgccatt tttagtgcta aaaccccggt
    11101 aatggatgca ctgtgggcca atcgtccgca taccctgatc gacgcttccg gtctggaagt
    11161 cggtctgcct gaccgtcaga tgggtaactc cgaagtaggt cacgttaacc tgggtgccgg
    11221 ccgcatcgtg tatcaggacc tgactcgtct ggacgttgaa atcaaagatc gcgctttctt
    11281 cgctaacccg gtactgactg gcgcggtaga taaagcgaaa aacgcaggca aagcagttca
    11341 cattatgggt ctgctctccg caggtggtgt acacagccac gaagatcaca tcatggcgat
    11401 ggtagaactg gcagctgaac gcggcgcaga aaaaatctac ctgcacgcat tccttgacgg
    11461 tcgcgacact ccgccgcgca gtgctgaatc ctcgctgaaa aaattcgaag agaaatttgc
    11521 cgcactgggc aaaggccgcg tagcgtccat cattggtcgt tactacgcca tggaccgcga
    11581 taaccgttgg gatcgcgttg aaaaagctta tgacctgctg actctggcgc agggcgagtt
    11641 ccaggccgat accgccgttg ctggtttgca ggctgcttat gctcgcgacg aaaacgacga
    11701 attcgtgaaa gcgaccgtta ttcgtgcaga aggtcagcca gacgcggcaa tggaagacgg
    11761 cgatgcgctg attttcatga acttccgtgc tgaccgtgca cgtgaaatca ctcgtgcatt
    11821 cgtaaatgcc gattttgacg gcttcgcgcg taagaaagtg gttaacgtcg atttcgtgat
    11881 gctgaccgaa tacgctgccg acatcaaaac cgcagtggct tacccacccg cttccctggt
    11941 taacaccttc ggcgagtgga tggcgaaaaa cgacaaaact cagctgcgta tttccgaaac
    12001 cgaaaaatat gcccacgtta ctttcttctt caacggtggc gtagaagagt cgttcaaagg
    12061 cgaagatcgc attctgatca actcaccgaa agtggctacc tacgatctgc aaccggaaat
    12121 gagctccgca gagctgaccg aaaaactggt tgcggcaatc aagagcggca aatacgacac
    12181 cattatctgt aactatccga acggcgacat ggtaggtcac accggagtga tggaagcggc
    12241 ggttaaagcg gttgaagcgc tggatcactg cgtggaagaa gtcgcgaaag cggttgagtc
    12301 cgtgggtgga caactgctga tcaccgctga ccacggtaac gctgagcaga tgcgcgatcc
    12361 ggcaacgggt caggcacaca cggcacatac caacctgcca gttccgctga tttacgttgg
    12421 cgataagaac gtgaaagcgg ttgaaggcgg caaactttct gatatcgcgc cgaccatgtt
    12481 gtcgctgatg ggtatggaaa tcccgcaaga gatgactggt aagccgctgt tcatcgtgga
    12541 ataatccctc cccatgaggg gaaaggcgat taataccatg acacgggccg tgaaaccgcg
    12601 caggtttgca atcaggccca tcatctacgc cagcgttctt agcgctggcg tattgttgtg
    12661 cgccttttcc gcccacgcgg atgagcgtga ccaactcaaa tctattcagg ccgatatcgc
    12721 cgcgaaagag cgcgcggtac gccaaaagca acaacaacgc gcaagcctgc tcgcacaatt
    12781 gaaaaagcag gaagaagcga tctcggaagc cacccgtaag ctgcgtgaaa cgcaaaacac
    12841 gctcaatcaa ctgaataaac agattgatga gatgaacgcg tcgattgcca aactggagca
    12901 gcaaaaagcc gcccaggagc gcagcctcgc cgcacaacta gatgccgcat tccgtcaggg
    12961 cgagcatacc ggtattcagc tgattctcag cggtgaagaa agccagcgtg gacagcgttt
    13021 acaggcttat ttcggctatc tcaaccaggc gcgacaagaa accattgctc agttgaaaca
    13081 aacgcgtgaa gaagtcgcta tgcaacgcgc cgagctggaa gagaaacaga gcgagcaaca
    13141 aacgctgttg tatgagcagc gcgcccaaca ggcgaagctg acccaggcgc tgaacgagcg
    13201 taaaaagacg ctggcagggc tggagtcttc catccagcaa ggtcagcaac agttgagcga
    13261 gctgcgcgcc aacgaatccc gtctgcgtaa cagcattgcc cgtgcggaag ccgcggcgaa
    13321 agcgcgtgct gaacgagaag cgcgtgaggc ccaggcggtt cgcgaccgcc agaaagaagc
    13381 gacgcgcaaa ggcaccacct acaaaccgac cgaaagcgaa aaatcgctga tgtcccgaac
    13441 tggtggcctg ggtgcgccgc gtggtcaggc attctggcca gttcgcgggc cgacgctgca
    13501 tcgctatggc gagcagctac agggtgaatt acgctggaaa ggaatggtta tcggtgcctc
    13561 tgaaggtact gaagttaaag ccattgccga cggtcgggtg attctggctg actggctgca
    13621 aggctacggt ctggtggtgg tggttgagca tggtaaaggc gacatgagtc tttacggcta
    13681 taatcagagc gcactggtga gcgttggttc gcaggttcgc gcgggccagc cgattgcact
    13741 ggtgggcagc agtggcggtc agggtcggcc ttcactctat ttcgaaattc gccgccaggg
    13801 tcaggcggtc aatccacagc cgtggttggg aagataagtt ttgtttccat ttcgtcgtaa
    13861 cgttcttgca tttgccgctc tgttggcgct ctcctccccc gtaattgctg gcaagcttgc
    13921 catcgtcatt gatgattttg ggtatcgccc gcacaacgaa aaccaggtgc tggcgatgcc
    13981 ttccgctatc tccgtcgctg tattacccga ttcaccgcac gccagagaga tggcaaccaa
    14041 agcgcataac agcggtcacg aagtgttgat tcatctaccg atggcaccac tgagtaaaca
    14101 gccgctggag aaaaatacgc tacgcccgga gatgagcagc gacgaaattg agcgcattat
    14161 tcgtagtgcg gtcaataacg tgccctatgc cgtggggatc aacaaccaca tgggcagcaa
    14221 gatgacctct aacctgtttg gtatgcagaa agtgatgcag gcgctggagc gttacaatct
    14281 ttacttcctc gacagcgtaa ccatcggtaa tacccaggcg atgcgcgcag cgcaaggcac
    14341 tggcgtgaag gtgatcaaac ggaaagtgtt cctcgacgat tcgcaaaatg aagcggacat
    14401 ccgtgtgcag ttcaatcgcg caattgatct ggcgcgtcgc aacggttcaa ccattgccat
    14461 tgggcatcct cacccttcaa cggtacgcgt gctacaacag atggtttata acctgccgcc
    14521 agacattacg ctggtgaaag ccagcagctt gctgaatgaa ccgcaggttg atacgtctac
    14581 accgccgaaa aacactgtgc ccgacacacc gcgtaatccg ttccgtggcg tgaagctctg
    14641 caaaccgaag aaaccgatag aacctgttta tgctaatcgc ttctttgaag tattaagcga
    14701 aagcatcagc cagagcacgc tgatcgttta cttccaacat cagtggcaag gctggggcaa
    14761 acagcctgaa gcggcaaaac ttaacgctag cgtaaattaa aggcgctacg cgtaatgcga
    14821 tggtgcgacc gcgttttgtc gcgccattgc cataagcgga aagaccacag taacgcctga
    14881 taacccacct tcacgctgcg cacattggta atcaggcgct tatacatacc ggaagtaaat
    14941 atctcggcaa tcatccgttg gcgggtaata atatccggct ctttgcgcac cgcatggcaa
    15001 acacgcaatg cttcgtaagt tatttgctga tgaaattccg gataaatcgt aattttgtcg
    15061 gcataatttc gatttaattt ctctagcagg cgggtaatct taatatagtg acgttgataa
    15121 ttaaggtttt tattcccttg tctattcaac cgactcactg acgtattatg cagataatat
    15181 ttatataacg attgctcggt atatcgcgca cgcagcgcat taaacataaa ttctgttgtc
    15241 cagacaatat cctgatgatg taatccggca ataaatttaa tgttattttt aacaataaca
    15301 tcgcggcgat aaacccccat ccagacaacg tgagtccaac ggcgggaaga aagccccatc
    15361 cgcaaccaat ccgggccggt taatacgccg gttgagcgaa ggcgatcggt ggggatggat
    15421 tgccaggttt ctcccgtttc acgaaaacac cagtcagcgt tgcactgcgc cacgtcgagg
    15481 tcgtcctcta acgccatggt catcagcgtt tcgtacatgg tgggatagac ctcatcgtca
    15541 gcatcgacaa aagcgacata tttgcccgtc gccacctcga tcccacgatt acgcgctacc
    15601 gatgcgccag cattcgcctg atgcaacaaa cgaacgtgcg gatagttttc tgcgtaatgc
    15661 tttgctattt caacagaatt atccgttgaa ccatcgttaa taataatgat ttccagagca
    15721 gtccaggttt gcgtaattaa agattccata cacgtgcgga aatcatcgcc cgcattatat
    15781 aacgggataa taacactaag tttattggtg ctgttcatca taaattaccg acgacctctg
    15841 aagttatcct gacgttttac aggctttgta ttaagaaact attaagcctg tgagatatta
    15901 tttttacagg tgagagagtc acttcagggt agagagataa tgagaacagc tatataagag
    15961 atcagcgcaa ttacgaactc tttattacac tgacaggatt ttacatattt aacggattat
    16021 cctcaggaaa aagggctgga ataccagccc ttgtttgtgt taatcccagc tcaggataac
    16081 tttcccggac tggcccgaac gcatggcgtc aaagcccttc tggaaatcat cgatagagaa
    16141 acgatgagta atgatcggcg aaagatcgag gccagactga atcagcgccg ccatcttgta
    16201 ccaggtttca aacatctcac gaccgtaaat acctttaatg aacaagcctt taaagatcac
    16261 tttggtccag tctatcgaca tgtcagaagg tggaataccc agcatcgcaa tacgaccgcc
    16321 gtggttcatg gtgtcaagca tggtacgaaa cgctggcggc gcaccggaca tttccagacc
    16381 gacatcaaag ccttcggtca tgcccagttc tgccatcaca tcattgaggt tctctttagc
    16441 gacgttaacc gcacgggtga tacccatttt acgcgccaac tcaaggcggt attcgttaac
    16501 atcagtaatc accacgtaac gtgcaccaac gtgtttcgcc accgccgccg ccataatacc
    16561 aatcgggcct gcaccagaga ccagcacatc ctcgcccacc agatcgaacg acagcgcggt
    16621 atgcacggcg ttaccgaagg gatcaaaaat ggaagccaga tcatcggaga tattgtcggg
    16681 gattttgaaa gcgttgaacg ccggaatcac cagatactcg gcgaagcagc ccggacggtt
    16741 aacgccgacg ccgatcgtgt tgcggcacag atgggtacga ccgccacgac agttgcgaca
    16801 gtgaccgcag gtgatatggc cttcgccaga aacgcgatcg ccaatcttga agcctttcac
    16861 ttcctgacca atacctacca cttcaccgac atattcatga cccacgacca tcggcaccgg
    16921 gatggttttt tgcgaccact catcccagtt atagatgtga acgtcagtcc cgcagatggc
    16981 tgttttacgg attttaatca gcagatcgtt atgcccgagt tccggtacag gaacgtcggt
    17041 catccagatg ccctcttccg ctttcagttt ggataacgct ttcatctcac atcctcaggc
    17101 gataacgccc agttgtttac caatacgcgt gaatgcttct actgcacgcg taatttgctc
    17161 aggggtatgc gccgcagaca tctgggtacg aatacgcgcc tgacctttcg gaacgaccgg
    17221 atagaagaaa ccggtgacgt aaatcccctc tttttgcagc tcacgggcaa atttctgcgc
    17281 cactaccgca tcgccaagca tgaccggaat aatggcgtga tcggctcccg ccagggtaaa
    17341 gcccgccgcc gacatttgct cacggaactg acgcgcgttc gcccacagac ggtcacgcag
    17401 ctcgctaccc gcttcaacca tctccagcac tttgatggaa gcggcaacaa tggccggtgc
    17461 cagcgagttg gagaacaggt acggacgaga acgctggcgc agccactcaa ccacttcttt
    17521 gcgcgccgcg gtataaccac cagaagcccc gcccagcgct ttaccaagcg taccggtgat
    17581 aatatcgacc cgccccatca catcgcagta ttcatgggaa ccacgaccgt tttcaccgac
    17641 aaaacctacc gcgtgtgagt catctaccat caccagggca tcatatttat ctgccagatc
    17701 gcaaacgcct ttcaggttgg caatcacgcc gtccatcgag aacacaccat cggtggcaat
    17761 cagcacatga cgcgcaccgg cttcacgcgc ttctttcaga cgtgcttcca gctcctgcat
    17821 atcgttgttg gcataacgat agcgtttagc tttgcacaga cgcacaccat caataataga
    17881 cgcgtggttc agtgcgtcgg agataatggc gtcttccgcg cccaacagcg tttcgaacag
    17941 gccaccgtta gcatcaaagc aggaagagta gagaatcgca tcttccatcc ccaggaaggc
    18001 cgccagtttt tgttcaagct ctttatggct gtcctgagtg ccgcaaataa aacgcaccga
    18061 agccatgccg aaaccgtgag aatccattcc cgcctttgcc gccgcaatca gatccggatg
    18121 attcgccagt ccgagatagt tattggcaca aaagttaatg acgtggcttc catcagccac
    18181 agtgatatct gcttgctgcg cagacgtaat aatgcgctct tctttaaaca acccttccgc
    18241 ccgtgcggtt tccagatcgt tggttaactg ctgataaaat tctccacgca ttgcgattct
    18301 ccagacaggg caaattccag cacatattac ccaaacttat aggttgcgac gagatgacgc
    18361 gttaacactt ctgcaaaatt caggataaat aacgtccagc cccctcactt ttggtgaatg
    18421 gctgaagcca acgtcatgta gtgatatgat agaagtattc gtgtctgaga ttgtctctga
    18481 ctccataatt cgaaggttac agttatgatc atcgttaccg gcggcgcggg ctttatcgga
    18541 agcaacatcg ttaaagccct gaatgataaa ggcatcaccg atattctggt ggtggacaac
    18601 ctgaaagacg gcaccaagtt tgtgaacctg gtggatctgg atatcgcgga ctatatggat
    18661 aaggaagact tcctgatcca gattatggct ggcgaagagt tcggcgatgt cgaagcgatt
    18721 ttccacgaag gcgcgtgctc ttccaccacc gagtgggacg gcaagtatat gatggataac
    18781 aactatcaat actccaaaga gctgctgcac tactgtctgg agcgcgaaat cccgttcctg
    18841 tatgcctctt ccgcagccac ttacggtgga cgcacctccg actttattga atcccgcgaa
    18901 tatgaaaaac cgttgaatgt ctacggttac tcaaaattcc tgtttgatga atatgttcgc
    18961 caaatcctgc ctgaagcgaa ctcacagatt gttggcttcc gttatttcaa cgtttatgga
    19021 ccgcgtgaag gccataaagg cagcatggcg agcgtcgctt tccatctcaa cacccagctt
    19081 aataacggtg aatcaccgaa gctgttcgaa ggtagcgaga acttcaaacg tgacttcgtc
    19141 tatgtgggcg atgtggcaga tgtaaacctg tggttcctgg aaaatggcgt ttccggcatc
    19201 ttcaacctcg gtactggtcg tgcggaatcc ttccaggcag tagcagatgc tacgcttgct
    19261 tatcacaaga aaggccaaat cgaatacatt ccgttcccgg ataaactgaa aggccgctac
    19321 caggcgttca cgcaggcaga tctgacaaat ctgcgcgcgg cgggttacga caaaccgttt
    19381 aaaaccgttg ccgaaggcgt aacggaatac atggcctggc tgaatcgtga cgcataagag
    19441 ctctgcatga aaatactggt gatcggcccg tcttgggttg gcgacatgat gatgtcgcaa
    19501 agtctctatc gcacgctcca ggcgcgctat ccccaggcga taatcgacgt gatggcaccg
    19561 gcatggtgcc gtccattatt atcgcggatg ccggaagtta acgaagctat tcctatgcct
    19621 ctcggtcacg gagcgctgga aatcggcgaa cgccgcaaac tgggtcatag cctgcgcgaa
    19681 aaacgttacg accgcgccta cgttttaccc aactccttca aatccgcatt agtgcctttc
    19741 ttcgcgggta ttcctcatcg caccggctgg cgcggcgaaa tgcgctacgg tttactcaac
    19801 gatgtgcgtg tgctggataa agaagcctgg ccgctgatgg tggaacgcta tgtggcgctg
    19861 gcctatgaca aaggtattat gcgtaccgca caagatctgc cgcagccatt gttatggccg
    19921 cagttgcagg tgagcgaagg tgaaaaatca tatacctgta atcaattttc gctttcatca
    19981 gaacgtccga tgattggttt ttgcccgggt gcggagtttg gtccggcaaa acgctggcca
    20041 cactaccact atgcggagct ggcaaagcaa ctgattgatg aaggttatca ggtggttctg
    20101 tttggctcgg cgaaagatca tgaagcgggc aatgagattc ttgccgcttt gaataccgag
    20161 cagcaggcat ggtgtcgtaa cctggcgggg gaaacacagc ttgatcaagc ggttatcctg
    20221 attgcagcct gtaaagccat tgttactaac gattctggcc tgatgcacgt tgcggcggcg
    20281 ctcaatcgtc cgctggttgc cctgtatggt ccgagtagcc cggacttcac accgccgcta
    20341 tcccataaag cgcgcgtgat ccgcctgatt accggctatc acaaagtgcg taaaggtgac
    20401 gctgcggagg gttatcacca gagcttgatc gacattactc cccagcgcgt actggaagaa
    20461 ctcaacgcgc tattgttaca agaggaagcc tgacggatgc gggttttgat cgttaaaaca
    20521 tcgtcgatgg gcgatgttct ccatacgttg cccgcactca ctgatgccca gcaggcaatc
    20581 ccagggatta agtttgactg ggtggtggaa gaagggttcg cacagattcc ttcctggcac
    20641 gccgccgttg agcgagttat tcctgtggca atacgtcgct ggcgtaaagc ctggttctcg
    20701 gcccccataa aagctgaacg caaagcgttt cgtgaagcgc tacaagcaga gaactatgac
    20761 gcagttatcg acgctcaggg gctggtaaaa agcgcggcgc tggtgacgcg tctggcgcat
    20821 ggcgtaaagc atggcatgga ctggcaaacc gctcgcgaac ctttagccag cctgttttac
    20881 aatcgtaagc atcatattgc aaaacagcag cacgccgtag aacgcacccg cgaactgttt
    20941 gccaaaagtc tgggctacag caaaccgcaa acccagggcg attatgctat cgcacagcat
    21001 ttcctgacga acctgcctac agatgctggc gaatatgccg tatttcttca tgcgacgacc
    21061 cgtgatgata aacactggcc ggaagaacac tggcgagaat tgattggttt actggctgat
    21121 tcaggaatac ggattaaact tccgtggggc gcgccgcatg aggaagaacg ggcgaaacga
    21181 ctggcggaag gatttgctta tgttgaagta ttgccgaaga tgagtctgga aggcgttgcc
    21241 cgcgtgctgg ccggggctaa atttgtagtg tcggtggata cggggttaag ccatttaacg
    21301 gcggcactgg atagacccaa tatcacggtt tatggaccta ccgatccggg attaattggt
    21361 gggtatggga agaatcagat ggtttgtagg gctccgggga atgagttgtc tcaattgaca
    21421 gcaaatgctg ttaagcaatt cattgaagaa aacgctgaaa aagctgccat gatttaaatt
    21481 acaactcttt aatagataat tttgaaataa aatcagcttc cttgctgatt ttatttcaca
    21541 tattc
//
LOCUS       DAZFLD010000070        21336 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.070, whole genome shotgun sequence.
ACCESSION   DAZFLD010000070 DAZFLD010000000
VERSION     DAZFLD010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21336
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.070"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..824
                     /locus_tag="ACHKBL_002227"
     CDS             <1..824
                     /locus_tag="ACHKBL_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HFO5819094.1"
                     /translation="AQPTIVPQLQQQVTDLTSSLNSQEKKELTRKLESIFNNTQVQIA
                     VLIIPTTKDETIEQYATRVFNNWRLGDAKRNDGILIIVAWSDRTVRIQVGYGLEEKVT
                     DALAGDIIRSNMIPAFKQQKLAKGLELAIIALNNQLTSQHQYSANPSESESASSSDHY
                     YFAIFWVFAVMFFPFWFFHQGSNFCRACKSSVCISAIYLLDLFLFSDKTFSIAVFSFF
                     FTFTIFMVFTCLCVLQKRASGRSYHSDNSGSAGGSGGGFSGGGGSSGGGGASGRW"
     gene            complement(884..2182)
                     /gene="eno"
                     /locus_tag="ACHKBL_002228"
     CDS             complement(884..2182)
                     /gene="eno"
                     /locus_tag="ACHKBL_002228"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HFO5819095.1"
                     /translation="MSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTG
                     SREALELRDGDKSRFLGKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTEN
                     KSKFGANAILAVSLANAKAAAAAKGMPLYEHIAELNGTPGKYSMPVPMMNIINGGEHA
                     DNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGS
                     NAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHF
                     LEELTKQYPIVSIEDGLDESDWDGFAYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKG
                     IANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQI
                     KTGSMSRSDRVAKYNQLIRIEEALGEKAPYNGRKEIKGQA"
     gene            complement(2270..3907)
                     /gene="pyrG"
                     /locus_tag="ACHKBL_002229"
     CDS             complement(2270..3907)
                     /gene="pyrG"
                     /locus_tag="ACHKBL_002229"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine hydrolyzing CTP synthase"
                     /protein_id="HFO5819096.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPY
                     INVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRTKMSRRNNFTTGRIYSDVLRK
                     ERRGDYLGATVQVIPHITNAIKERVLEGGEGHDVVLVEIGGTVGDIESLPFLEAIRQM
                     AVEIGREHTLFMHLTLVPYMAASGEVKTKPTQHSVKELLSIGIQPDILICRSDRAVPA
                     NERAKIALFCNVPEKAVISLKDVDSIYKIPGLLKSQGLDDYICKRFSLNCPEANLSEW
                     EQVIFEEANPVSEVTIGMVGKYIELPDAYKSVIEALKHGGLKNRVSVNIKLIDSQDVE
                     TRGVEILKGLDAILVPGGFGYRGVEGMITTARFARENNIPYLGICLGMQVALIDYARH
                     VANMENANSTEFVPDCKYPVVALITEWRDENGNVEVRSEKSDLGGTMRLGAQQCQLVD
                     DSLVRQLYNAPTIVERHRHRYEVNNMLLKQIEDAGLRVAGRSGDDQLVEIIEVPNHPW
                     FVACQFHPEFTSTPRDGHPLFAGFVKAASEFQKRQAK"
     gene            complement(4135..4926)
                     /gene="mazG"
                     /locus_tag="ACHKBL_002230"
     CDS             complement(4135..4926)
                     /gene="mazG"
                     /locus_tag="ACHKBL_002230"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HFO5819097.1"
                     /translation="MNQIDRLLTIMQRLRDPENGCPWDKEQTFATIAPYTLEETYEVL
                     DAIAREDFDDLRGELGDLLFQVVFYAQMAQEEGRFDFNDICAAISDKLERRHPHVFAD
                     GSAGNSREVLARWEQIKTEERAQKAQHSALDDIPSSLPALMRAQKIQKRCANVGFDWT
                     TLGPVVDKVYEEIDEVMYEARQVVVDQAKLEEEMGDLLFATVNLARHLGTKAEIALQK
                     ANEKFERRFREVERIVAARGLEMTGVDLETMEEVWQQVKRQEIDL"
     gene            complement(5022..7256)
                     /gene="relA"
                     /locus_tag="ACHKBL_002231"
     CDS             complement(5022..7256)
                     /gene="relA"
                     /locus_tag="ACHKBL_002231"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP diphosphokinase"
                     /protein_id="HFO5819098.1"
                     /translation="MVAVRSAHINKAGEFDPEKWIASLGITSQKSCECLAETWAYCLQ
                     QTQGHPDASLLLWRGVEMVEILSTLSMDIDTLRAALLFPLADANVVSEDVLRESVGKS
                     VVNLIHGVRDMAAIRQLKATHTDSVSSEQVDNVRRMLLAMVDDFRCVVIKLAERIAHL
                     REVKDAPEDERVLAAKECTNIYAPLANRLGIGQLKWELEDYCFRYLHPTEYKRIAKLL
                     HERRLDREHYIEEFVGHLRAEMKAEGVKAEVYGRPKHIYSIWRKMQKKNLAFDELFDV
                     RAVRIVAERLQDCYAALGIVHTHYRHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTV
                     EIQIRTKQMHEDAELGVAAHWKYKEGAAAGGARSGHEDRIAWLRKLIAWQEEMADSGE
                     MLDEVRSQVFDDRVYVFTPKGDVVDLPAGSTPLDFAYHIHSDVGHRCIGAKIGGRIVP
                     FTYQLQMGDQIEIITQKQPNPSRDWLNPNLGYVTTSRGRSKIHAWFRKQDRDKNILAG
                     RQILDDELEHLGISLKEAEKHLLPRYNFNDVDELLAAIGGGDIRLNQMVNFLQSQFNK
                     PSAEEQDAAALKQLQQKSYTPQNRSKDNGRVVVEGVGNLMHHIARCCQPIPGDEIVGF
                     ITQGRGISVHRADCEQLAELRSHAPERIVDAVWGESYSAGYSLVVRVVANDRSGLLRD
                     ITTILANEKVNVLGVASRSDTKQQLATIDMTIEIYNLQVLGRVLGKLNQVPDVIDARR
                     LHGS"
     gene            complement(7304..8605)
                     /gene="rlmD"
                     /locus_tag="ACHKBL_002232"
     CDS             complement(7304..8605)
                     /gene="rlmD"
                     /locus_tag="ACHKBL_002232"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFO5819099.1"
                     /translation="MAQFYSAKRRTTTRQIITVSVNDLDSFGQGVARHNGKALFIPGL
                     LPQENAEVTVTEDKKQYARAKVVRRLSDSPERETPRCPHFGVCGGCQQQHASVDLQQR
                     SKSAALARLMKHEVSEVIADVPWGYRRRARLSLNYLPKTQQLQMGFRKAGSSDIVDVK
                     QCPILVPQLEALLPKVRACLGSLQAIRHLGHVELVQATSGTLMILRHTAPLSSADREK
                     LERFSHSEGLDLYLAPDSEILETVSGEMPWYDSNGLRLTFSPRDFIQVNAGVNQKMVA
                     RALEWLDVEPEDRVLDLFCGMGNFTLPLATQAASVVGVEGVPALVEKGQQNARLNGLQ
                     NVTFYHENLEEDVTKQPWAKNGFDKVLLDPARAGAAGVMQQIIKLEPIRIVYVSCNPA
                     TLARDSEALLKAGYTIARLAMLDMFPHTGHLESMVLFSRVK"
     gene            8662..11418
                     /gene="barA"
                     /locus_tag="ACHKBL_002233"
     CDS             8662..11418
                     /gene="barA"
                     /locus_tag="ACHKBL_002233"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase BarA"
                     /protein_id="HFO5819100.1"
                     /translation="MTNYSLRARMMILILAPTVLIGLLLSIFFVVHRYNDLQRQLEDA
                     GASIIEPLAVSTEYGMSLQNRESIGQLISVLHRRHSDIVRAISVYDENNRLFVTSNFH
                     LDPSSMQLGSNVPFPRQLTVTRDGDIMILRTPIISESYSPDESPSSDAKNSQNMLGYI
                     ALELDLKSVRLQQYKEIFISSVMMLFCIGIALIFGWRLMRDVTGPIRNMVNTVDRIRR
                     GQLDSRVEGFMLGELDMLKNGINSMAMSLAAYHEEMQHNIDQATSDLRETLEQMEIQN
                     VELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTI
                     ERSANNLLAIINDVLDFSKLEAGKLILESIPFPLRSTLDEVVTLLAHSSHDKGLELTL
                     NIKSDVPDNVIGDPLRLQQIITNLVGNAIKFTENGNIDILVEKRALSNTKVQIEVQIR
                     DTGIGIPERDQSRLFQAFRQADASISRRHGGTGLGLVITQKLVNEMGGDISFHSLPNR
                     GSTFWFHINLDLNPNIIIEGPSTQCLAGKRLAYVEPNSAAAQCTLDILSETPLEVVYS
                     PTFSALPPAHYDMMLLGIAVTFREPLTMQHERLAKAVSMTDFLMLALPCHAQVNAEKL
                     KQDGIGACLLKPLTPTRLLPALTEFCHHKQNTLLPVTDESKLAMTVMAVDDNPANLKL
                     IGALLEDMVQHVELCDSGHQAVERAKQMPFDLILMDIQMPDMDGIRACELIHQLPHQQ
                     QTPVIAVTAHAMAGQKEKLLGAGMSDYLAKPIEEERLHNLLLRYKPGSGISSRVVTPE
                     VNEIVVNPNATLDWQLALRQAAGKTDLARDMLQMLLDFLPEVRNKVEEQLVGENPEGL
                     VDLIHKLHGSCGYSGVPRMKNLCQLIEQQLRSGTKEEDLEPELLELLDEMDNVAREAS
                     KILG"
     gene            complement(11642..12982)
                     /gene="gudD"
                     /locus_tag="ACHKBL_002234"
     CDS             complement(11642..12982)
                     /gene="gudD"
                     /locus_tag="ACHKBL_002234"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008872 - glucarate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0019394 - glucarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HFO5819101.1"
                     /translation="MSSQFSTPVVTEMQVIPVAGHDSMLMNLSGAHAPFFTRNIVIIK
                     DNSGHTGVGEIPGGEKIRKTLEDAIPLVVGKTLGEYKNVLTLVRNTFADRDAGGRGLQ
                     TFDLRTTIHVVTGIEAAMLDLLGQHLGVNVASLLGDGQQRSEVEMLGYLFFVGNRKAT
                     PLPYQSQPDDKCDWYRLRHEEAMTPDAVVRLAEAAYEKYGFNDFKLKGGVLAGEEEAE
                     SIVALAQRFPQARITLDPNGAWSLNEAIKIGKYLKGSLAYAEDPCGAEQGFSGREVMA
                     EFRRATGLPTATNMIATDWRQMGHTLSLQSVDIPLADPHFWTMQGSVRVAQMCHEFGL
                     TWGSHSNNHFDISLAMFTHVAAAAPGKITAIDTHWIWQEGNQRLTKEPFEIKGGLVQV
                     PQKPGLGVEIDMDQVMKAHELYQKHGLGARDDAMGMQYLIPGWTFDNKRPCMVR"
     gene            complement(13003..14343)
                     /locus_tag="ACHKBL_002235"
     CDS             complement(13003..14343)
                     /locus_tag="ACHKBL_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HFO5819102.1"
                     /translation="MTTQSSPVITDMKVIPVAGHDSMLLNIGGAHNAYFTRNIVVLTD
                     NAGHTGIGEAPGGEVIYQTLVKAIPMVLGQEVARLNKVVQQVHKGNQAADFDTFGKGA
                     WTFELRVNAVAALEAALLDLLGQALNVPVCELLGPGKQRDAITVLGYLFYIGDRTKTD
                     LPYLENTPGNHEWYQLRHQKAMNSEAVVRLAEASQDRYGFKDFKLKGGVLPGEQEIDT
                     VRALKKRFPDARITVDPNGAWLLDEAISLCKGLNDVLTYAEDPCGAEQGFSGREVMAE
                     FRRATGLPVATNMIATNWREMGHAVMLNAVDIPLADPHFWTLSGAVRVAQLCDDWGLT
                     WGCHSNNHFDISLAMFTHVGAAAPGNPTAIDTHWIWQEGDCRLTKNPLEIKNGKIAVP
                     DAPGLGVGLDWEQVQKAHEAYKRLPGGARNDAGPMQYLIPGWTFDRKRPVFGRH"
     gene            complement(14345..15697)
                     /gene="gudP"
                     /locus_tag="ACHKBL_002236"
     CDS             complement(14345..15697)
                     /gene="gudP"
                     /locus_tag="ACHKBL_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GudP"
                     /protein_id="HFO5819103.1"
                     /translation="MSSLSQAASSVEKRTNARYWIVVMLFIVTSFNYGDRATLSIAGS
                     EMAKDIGLDPVGMGYVFSAFSWAYVIGQIPGGWLLDRFGSKRVYFWSIFIWSMFTLLQ
                     GFVDIFSGFGIIVALFTLRFLVGLAEAPSFPGNSRIVAAWFPAQERGTAVSIFNSAQY
                     FATVIFAPIMGWLTHEVGWSHVFFFMGGLGIVISFIWLKVIHEPNQHPGVNQKELEYI
                     AAGGALINMDQQNTKVKVPFSVKWGQIKQLLGSRMMIGVYIGQYCINALTYFFITWFP
                     VYLVQARGMSILKAGFVASVPAVCGFIGGVLGGIISDWLMRRTGSLNIARKTPIVMGM
                     LLSMVMVFCNYVNVEWMIIGFMALAFFGKGIGALGWAVMADTAPKEISGLSGGLFNMF
                     GNISGIVTPIAIGYIVGTTGSFNGALIYVGVHALIAVLSYLVLVGDIKRIELKPVAGQ
                     "
     gene            complement(16132..16581)
                     /locus_tag="ACHKBL_002237"
     CDS             complement(16132..16581)
                     /locus_tag="ACHKBL_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HFO5819104.1"
                     /translation="MAEIGIFVGTMYGNSLLVAEEAEAILTAQGHKATVFEDPELSDW
                     LPYQDKYVLVVTSTTGQGDLPDSIVPLFQGIKDTLGFQPNLRYGVIALGDSSYVNFCN
                     SGKQFDALLQEQSAQRVGEMLLIDASENPEPETESNPWVEHWGTMLS"
     gene            complement(16599..17381)
                     /gene="truC"
                     /locus_tag="ACHKBL_002238"
     CDS             complement(16599..17381)
                     /gene="truC"
                     /locus_tag="ACHKBL_002238"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HFO5819105.1"
                     /translation="MLEILYQDEWLVAVNKPSGWLVHRSWLDRDEKVVVMQTVRDLIG
                     QHVFTAHRLDRPTSGVLLMGLSSEAGRLLAQQFEQHQIQKRYHAIVRGWLMEEAVLDY
                     PLVEELDKIADKFAREDKGPQPAVTHYRGLATVEMLVATGRYPTTRYGLVELEPKTGR
                     KHQLRRHLAHLRHPIIGDSKHGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGEPLT
                     IHAGLDDTWMQALSQFGWRGLLPENERVEFSAPSGQDGEISS"
     gene            complement(17381..17710)
                     /locus_tag="ACHKBL_002239"
     CDS             complement(17381..17710)
                     /locus_tag="ACHKBL_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HFO5819106.1"
                     /translation="MTTHDRVRLQLQALEALLREHQHWRNDKPQPHQFNSTQPFFMDT
                     MEPLEWLQWVLIPRMHDLLDNNQPLPGAFAVAPYYEMALATDHPQRALILAELEKLDA
                     LFADDAS"
     gene            complement(18332..18877)
                     /gene="syd"
                     /locus_tag="ACHKBL_002240"
     CDS             complement(18332..18877)
                     /gene="syd"
                     /locus_tag="ACHKBL_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein"
                     /protein_id="HFO5819107.1"
                     /translation="MDDLTAQALKDFTARYCDAWHEEHKSWPLSEELYGVPSPCIIST
                     TEDAVYWQPQPFTGEQNVNAVERAFDIVIQPTIHTFYTTQFAGDMHAQFGDIKLTLLQ
                     TWSEDDFRRVQENLIGHLVTQKRLKLPPTLFIATLEEELEVISVCNLSGEVCKETLGT
                     RKRTHLASNLAEFLNQLKPLL"
     gene            18945..19793
                     /gene="queF"
                     /locus_tag="ACHKBL_002241"
     CDS             18945..19793
                     /gene="queF"
                     /locus_tag="ACHKBL_002241"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708588.1"
                     /note="Catalyzes the NADPH-dependent reduction of
                     7-cyano-7-deazaguanine (preQ0) to
                     7-aminomethyl-7-deazaguanine (preQ1) in queuosine
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA];
                     GO_function: GO:0043867 - 7-cyano-7-deazaguanine
                     tRNA-ribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HFO5819108.1"
                     /translation="MSSYANHQALAGLTLGKSTDYRDTYDASLLQGVPRSLNRDPLGL
                     KADNLPFHGTDIWTLYELSWLNAKGLPQVAVGHVELDYTSANLIESKSFKLYLNSFNQ
                     TRFNNWDEVRQTLERDLSTCAQGKVSVALYRLDELEGQPIGHFNGTCIDDQDITIDNY
                     EFTTDYLENATSGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRQIDREKLLRY
                     LVSFRHHNEFHEQCVERIFNDLLRFCQPEKLSVYARYTRRGGLDINPWRSNSDFVPST
                     TRLVRQ"
     gene            19905..21269
                     /gene="ppnN"
                     /locus_tag="ACHKBL_002242"
     CDS             19905..21269
                     /gene="ppnN"
                     /locus_tag="ACHKBL_002242"
                     /EC_number="3.2.2.10"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase PpnN"
                     /protein_id="HFO5819109.1"
                     /translation="MITHISPLGSMDMLSQLEVDMLKRTASSDLYQLFRNCSLAVLNS
                     GSLTDNSKELLSRFENFDINVLRRERGVKLELINPPEEAFVDGRIIRALQANLFAVLR
                     DILFVYGQIHNTVRFPNLNLDNSVHITNLVFSILRNARALHVGEAPNMVVCWGGHSIN
                     ENEYLYARRVGNQLGLRELNICTGCGPGAMEAPMKGAAVGHAQQRYKDSRFIGMTEPS
                     IIAAEPPNPLVNELIIMPDIEKRLEAFVRIAHGIIIFPGGVGTAEELLYLLGILMNPA
                     NKDQVLPLILTGPKESADYFRVLDEFVVHTLGENARRHYRIIIDDAAEVARQMKKSMP
                     LVKENRRDTGDAYSFNWSMRIAPDLQMPFEPSHENMANLKLYPDQPVEVLAADLRRAF
                     SGIVAGNVKEVGIRAIEEFGPYKINGDKEIMRRMDDLLQGFVAQHRMKLPGSAYIPCY
                     EICT"
BASE COUNT         5232 a         5702 c         5113 g         5289 t
ORIGIN      
        1 cagcccaacc aactattgtc ccacaattac agcaacaagt tacggatctt acgagtagct
       61 taaactcaca agaaaagaaa gaactgacgc ggaagttaga atctattttc aataataccc
      121 aagtacaaat tgctgtatta attattccca caaccaaaga cgagacaatt gaacaatatg
      181 ctacaagggt ttttaacaat tggcgtttag gagatgccaa acgcaatgat gggatactga
      241 tcattgttgc ctggtcggat cgcactgtcc gcatccaggt aggttatggg ctggaagaaa
      301 aggtaaccga tgctctggct ggagatatca tccgtagcaa catgataccc gcatttaaac
      361 aacaaaaatt agctaaggga ttagagttag ctataatcgc tttgaataac caactcactt
      421 cacaacacca atattcggct aatccttcag aaagtgaatc agcgtcttcc agcgatcatt
      481 actactttgc tattttttgg gtatttgcag tgatgttctt ccctttctgg ttttttcatc
      541 aaggcagtaa tttttgtcgc gcatgtaaaa gcagtgtctg tatttcagca atctatcttt
      601 tagatttatt cctgttctcc gataaaactt tttccattgc tgtattttcc ttctttttta
      661 ctttcaccat atttatggtc tttacctgtt tatgtgtact tcagaaaaga gcatcaggta
      721 gaagttatca ttcagacaac agcggttccg ctggaggctc aggcggcggg tttagcggcg
      781 gtggcggttc ttctggcggt ggcggcgcat ccggccgctg gtaactcaac cataaaaaac
      841 gccagcccga aggctggcat ttttaaatca gataaagtca ggcttatgcc tggcctttga
      901 tctctttacg accgttgtac ggtgcttttt cgcccagagc ttcttcgata cgaatcagct
      961 ggttgtattt agcaacacgg tcagaacggc tcatagaacc agttttgatc tggcctgcag
     1021 cagtaccaac agccaggtca gcgatagtag cgtcttcagt ttcgccagaa cggtgagaga
     1081 taactgcagt gtagccagcg tctttcgcca tcttgattgc agccagagtt tcggtcagag
     1141 aaccgatctg gttgaatttg atcaggatgg agttagcgat acctttttcg ataccttctt
     1201 tcaggatctt ggtgttggtt acgaacaggt cgtcaccaac cagctggatt ttgtcgccca
     1261 gaactttggt ctggtatgcg aaaccgtccc agtcagattc gtccagaccg tcttcgatag
     1321 atacgatcgg atactgtttg gtcagttctt ccaggaagtg agtgaattct tcagaggtga
     1381 acgctttgtt gccttcgcca gccagaacgt atttaccgtc tttgtagaat tcagaagctg
     1441 cgcagtccat cgccagagtg atgtctttgc ccagttcata accagcagct ttaacagctt
     1501 cagcgataac agccagagct tcagcgttgg aacccaggtt cggcgcatag ccaccttcgt
     1561 caccaactgc agtgttcatg cctttcgctt tcagaacttt tgccaggtga tggaaaactt
     1621 cagaacccat gcggatggct tctttcacag ttttcgcgcc aaccggctga atcatgaatt
     1681 cctggatatc aacgttgttg tcagcgtgct caccaccgtt gatgatgttc atcatcggaa
     1741 ccggcatgga gtatttgccc ggagtgccgt tcagttcagc gatgtgctcg tacagcggca
     1801 tacctttagc agctgcagca gctttggcgt tagccagaga tacagccagg attgcgttcg
     1861 cgccgaattt ggatttgttt tcggtgccgt ccaggtcgat catgatcttg tcaatgccag
     1921 cctgatcttt agcatctttg ccaatcagcg cctgagcgat cgggccgttt accgcagcaa
     1981 cagctttggt tacgccttta cccaggaaac gggatttgtc gccatcgcgc agttccagag
     2041 cttcacggga accagtagaa gcacctgacg gagcagctgc cataccgacg aaaccaccct
     2101 ccagatgtac ttcggcttca acagtcgggt taccacggga gtcgatgatt tcacgaccga
     2161 tgatttttac gattttggac attaggtttt cctcaagtca ctagttaaac tgaaactcca
     2221 gacaaacaac gcgtacccag ggtacgcgtt gccgttctaa cttttttact tacttcgcct
     2281 gacgtttctg gaactcgctg gcggctttca caaagcctgc aaacagcggg tgaccatcac
     2341 gtggagtaga agtaaactcc ggatggaact ggcaagccac gaaccacggg tgattcggaa
     2401 cttcgatgat ctcgaccaac tgatcatccc cggaacggcc cgcaacgcgc agacctgcat
     2461 cttcaatctg tttcaacagc atgttgttga cttcgtaacg gtgacgatga cgctcaacaa
     2521 ttgtcggcgc attgtacagc tggcgaacca ggctatcgtc aaccaactgg cactgctgtg
     2581 cgccgagacg catggtaccg ccgagatcgc tcttctcgct acgaacttca acgttgccgt
     2641 tttcatcgcg ccactcggta atcagcgcca caactgggta cttacagtct ggcacaaatt
     2701 ccgtagagtt ggcgttctcc atgttggcaa catggcgagc gtaatcaatt aacgccacct
     2761 gcatacccag gcaaataccc agataaggaa tattgttctc acgcgcaaaa cgcgcggtcg
     2821 taatcatgcc ttctacgcca cgatagccga aaccgccagg tacgaggatt gcgtccagac
     2881 ctttaaggat ttcaacgccg cgcgtttcaa catcttgtga atcgatcagt ttgatgttga
     2941 cgctgacacg attcttcagc ccaccgtgtt tcagtgcttc gatcactgat ttataagcat
     3001 ccggcagttc aatgtacttg ccgaccatac cgatggtgac ttcacttacc ggattcgctt
     3061 cttcgaagat aacctgttcc cattcggaca gatttgcttc cgggcagttt aagctgaatc
     3121 gtttacaaat ataatcgtcc agcccctgag atttcaacag gcccggaatt ttatagatgg
     3181 aatcgacgtc tttcagagaa ataaccgctt tttccggaac attacagaac aatgcaatct
     3241 tcgcacgttc gttcgccgga acggcgcgat ctgaacgaca aatcaggatg tcaggctgga
     3301 taccgatgga gagcagctct tttacagagt gctgagtcgg tttggttttg acttcaccag
     3361 acgctgccat gtacggcacc agcgtcaggt gcataaacag agtgtgctca cggccaattt
     3421 caacagccat ctggcgaatc gcttcgagga acggcaggga ttcgatatca cctacagtac
     3481 cgccgatttc gaccagtact acgtcatgac cttcgccgcc ttccagcacg cgctctttga
     3541 ttgcgttagt gatgtgcgga ataacctgca cggttgcgcc gaggtagtca ccgcggcgtt
     3601 ctttacgcag aacgtcagag tagatacgac ccgtggtgaa gttgttgcgg cggctcattt
     3661 tggtacgaat gaaacgctcg tagtgcccca ggtccaggtc ggtttcagcg ccgtcttcag
     3721 taacgaacac ttccccgtgt tggattgggc tcatagtacc tggatcgacg ttgatgtacg
     3781 gatccagttt catgatggtc acattgaggc cacgggcttc aagaatggct gcgagggagg
     3841 ctgcggcaat gcctttaccc agagaggata cgaccccgcc ggtcacaaaa atatagttcg
     3901 ttgtcatgct gaacctgaga agttaggttg aaaagacgat ggaataacca ggacgggaaa
     3961 gcagtatacc cgaacatgac ctgtgccaca aactttcatt atccctcctc ttcgccagcg
     4021 cactattgaa atcaggagtg agaaaatagc ccctttgggg taaatgtttt tgacgcaaat
     4081 caagcgcttg tcatttaaaa aatgacacaa atcgcgcttg accacgtaat tcccttagag
     4141 atcaatttcc tgccgtttta cctgttgcca gacttcttcc attgtttcga ggtcaacacc
     4201 tgtcatttcc agtccacgcg cggcaacaat acgctccact tcgcgaaaac gacgctcgaa
     4261 tttttcgttc gctttttgca atgcgatttc cgcttttgtc cctaaatgac gagccagatt
     4321 gactgtggca aacagcaggt cacccatttc ctcctccagt ttagcctggt cgacaacaac
     4381 ctgccgcgct tcgtacatca cctcatcgat ctcttcgtag actttatcga ctaccgggcc
     4441 aagcgtcgtc cagtcgaagc caacgttggc gcaacgtttc tggatttttt gtgcgcgcat
     4501 taaggcgggt aaactactag ggatatcgtc cagcgccgaa tgctgcgctt tttgcgcgcg
     4561 ctcttcagtt ttgatttgct cccaacgagc aagaacttcg cggctgtttc cggcagaacc
     4621 gtcagcaaaa acatgtggat gacggcgttc taatttatcg ctaatcgcag cgcaaatatc
     4681 attaaaatca aagcgccctt cttcctgggc catttgtgcg taaaacacca cctggaacag
     4741 cagatcgccc agttcaccgc gcagatcgtc aaaatcttca cgggcgatgg cgtccagcac
     4801 ctcgtaggtt tcttcaaggg tgtaaggcgc aatggtggca aatgtctgct ctttatccca
     4861 cgggcagccg ttttccgggt cacgcaggcg ctgcatgatg gtgagcaaac ggtcgatttg
     4921 attcattaga atgtcctgaa aattgcgggt ccgtcaggtg taataccttc gagtaaaaga
     4981 tatcgctgga tacggctgac gccatatcca gctatgtaag actaactccc gtgcaaccga
     5041 cgcgcgtcga taacatccgg cacctggttg agtttaccca gcacgcgccc cagcacttgc
     5101 aggttgtaaa tctcaatggt catgtcgatg gtcgccagtt gctgtttggt gtcgctacgg
     5161 ctggcaacgc caagtacgtt caccttctcg ttggcgagaa tggtcgtgat atcacgtaac
     5221 aacccactac gatcattagc caccacgcgg accaccagcg aatatccggc ggagtagctc
     5281 tcgccccata ccgcgtcaac aatgcgttct ggcgcatggg agcgcagttc cgccaattgt
     5341 tcgcaatcgg cgcggtgtac tgaaataccg cgcccctggg taatgaagcc gacaatctca
     5401 tctccaggaa tcggctggca gcagcgcgcg atgtggtgca tcaggttacc gaccccttct
     5461 accaccacac gaccgttatc tttactgcgg ttttgcggcg tgtagctttt ttgctgaagc
     5521 tgtttcagcg cggcggcgtc ctgctcttcg gcactcggct tattaaattg cgattgcagg
     5581 aagttcacca tctgattgag acggatatcc ccgccaccaa tcgccgccag caactcgtcg
     5641 acatcattga agttgtaacg cggcagcaga tgtttttctg cttctttcag gctgatcccc
     5701 agatgttcca gctcgtcgtc aagaatttgc cgcccagcca gaatgttttt gtcacggtcc
     5761 tgtttacgga accaggcgtg aattttcgaa cgcccacggc tggttgtgac gtaaccgagg
     5821 tttgggttta accagtcacg gctggggttc ggctgtttct gggtgataat ttcaatctgg
     5881 tcgcccatct gcagctggta ggtgaacggc acaatgcgcc cgccaatttt tgccccgatg
     5941 cagcggtgtc cgacatcact gtggatgtgg taagcgaagt ccagcggcgt tgatcccgca
     6001 ggcaaatcaa cgacatcacc tttcggcgta aagacgtaca ctcggtcgtc aaagacctga
     6061 ctacgtactt cgtcgagcat ttcgccggaa tcagccatct cttcctgcca cgcaatcagt
     6121 ttacgcagcc aggcaatccg gtcttcatgt cccgaacgtg cgccgccagc agccgcgccc
     6181 tctttatatt tccagtgcgc agcaacaccc aactctgcat cttcatgcat ctgtttggtg
     6241 cggatttgga tctcaacggt tttgccaccc ggccccagaa ccacggtatg aatggactga
     6301 taaccgtttg gtttcgggtt agcgacgtaa tcgtcaaact catccggcag gtggcgatag
     6361 tgagtgtgca ctatccccag tgcggcataa caatcctgta aacgctcggc gacaatacgt
     6421 accgcacgca catcaaacag ctcatcgaag gcgaggttct ttttctgcat tttgcgccag
     6481 atactgtaga tgtgtttcgg acggccatac acttcagctt taacgccttc agctttcatc
     6541 tcagcgcgca gatggccgac aaactcttcg atatagtgtt cgcggtcgag acggcgttca
     6601 tgcagcagtt ttgcgatgcg tttgtattcg gtcgggtgga gataacggaa gcagtaatct
     6661 tccagctccc atttcagttg cccaatccca agacggttcg ccaacggcgc gtagatattg
     6721 gtgcactctt ttgccgccag tacgcgttca tcttccggcg catcttttac ttcgcgcagg
     6781 tgagcaatac gctccgccag tttgatgacc acgcagcgaa aatcatcgac catcgccaat
     6841 aacatccggc gaacgttatc gacctgttcg gaggaaacag aatcagtgtg cgtcgctttc
     6901 agctggcgga tcgccgccat atcacgcacg ccgtgaataa ggttaacgac cgacttaccg
     6961 acgctctcac gcagcacatc ttcgctgact acgttggcat cagccagagg gaacagcagc
     7021 gccgcccgca gcgtgtcaat gtccatactt aatgtcgaga ggatctccac catctcaaca
     7081 ccacgccaca ataacagact ggcatccgga tgcccctgcg tctgttgcag acaatacgcc
     7141 caggtttcgg ctaagcactc acacgacttc tggctggtaa tacccagact tgcgatccat
     7201 ttttccggat caaattcacc agccttattg atatgtgcac ttcttaccgc aaccatcgtc
     7261 ctctccttta gggaccagac ctgccgaaat cggcaaatcg taactattta acgcgcgaga
     7321 aaagtaccat cgattccaga tgtcccgtgt gtgggaacat atccagcatc gccagtcgcg
     7381 caatggtata tcctgctttt aataacgctt cgctatcccg agccagcgtt gcagggttac
     7441 aggatacata aactatacga ataggttcca gttttataat ttgctgcata acacctgcgg
     7501 cacctgctcg cgccgggtcc aacaacactt tatcgaagcc gtttttcgcc cacggctgct
     7561 ttgtgacatc ttcttcaaga ttttcgtgat aaaacgtcac attctgtaag ccattaagac
     7621 gcgcattctg ctggcctttt tccaccagcg ccggaacacc ttctacaccc accacactag
     7681 cagcttgtgt cgccaatggc agtgtaaagt tgcccatacc gcagaacaga tccagtacgc
     7741 gatcttcagg ttccacatcc agccattcca acgcacgcgc taccattttc tggttcacac
     7801 ccgcattgac ctgaataaaa tcgcgcgggc taaaagttaa gcgcaaccca tttgagtcat
     7861 accagggcat ctcaccagag acggtttcga gtatctcact atcgggggcg agatacagat
     7921 ccaggccttc agaatgcgaa aagcgttcca gtttttcgcg atcagccgaa cttagcggtg
     7981 cggtatggcg caaaatcatc agcgtgccgc tggttgcctg taccagttca acatgaccaa
     8041 gatggcgtat agcttgtaag ctgcccaggc atgccctgac tttgggcagc aatgcttcaa
     8101 gttggggcac taaaatggga cattgtttaa cgtcgacaat gtcactggag cccgctttgc
     8161 gaaaccccat ctgaagttgc tgtgttttcg gtaagtagtt cagacttaaa cgcgcgcgac
     8221 ggcgatagcc ccagggaaca tcggcgatca cttcagagac ttcgtgtttc attaatcggg
     8281 cgagtgccgc acttttgctt cgctgctgta aatccacgct ggcgtgttgt tgctgacagc
     8341 cgccgcatac gccaaaatga ggacagcgtg gcgtttcgcg ttccgggcta tcgcttaacc
     8401 ggcgcacgac tttagcgcgg gcgtattgtt ttttatcttc agtaacagta acttccgcgt
     8461 tttcctgtgg caataatcca ggaataaata acgctttgcc gttatgtcgt gccacgccct
     8521 gaccaaaaga gtcgaggtcg ttgactgaaa cagttatgat ctgacgcgtc gtcgtgcgtc
     8581 gttttgcaga gtagaattgc gccattggcg agactttctc aatttaacag tgtgacctta
     8641 attgtcccat aacggaactc catgaccaac tacagcctgc gcgcacgcat gatgattctg
     8701 atcctggcac cgaccgtcct tattggttta ttgctgagta tctttttcgt tgtgcatcgc
     8761 tataacgact tgcagcgtca actggaagat gccggagcca gcattattga gccgcttgca
     8821 gtttctactg aatatggcat gagcctgcaa aatcgcgaat ctatcggtca gttaataagc
     8881 gtactgcatc gtcgccattc cgatattgtt cgcgcgattt cggtttatga tgaaaataac
     8941 cgactctttg tcacctccaa ttttcatctt gatccctcat caatgcagct cggcagcaac
     9001 gtgccgtttc ctcgccagct cactgtcact cgtgacggcg atattatgat cctccgcacg
     9061 ccgattattt ctgaaagtta ctcccccgac gaatcgccca gtagcgatgc caaaaatagt
     9121 caaaatatgc tgggatatat tgcgctggag ctggatctta aatcggttcg cttgcagcaa
     9181 tataaagaga tctttatttc cagcgtgatg atgctgtttt gtatcggtat tgcgcttatt
     9241 tttggctggc gcttaatgcg cgatgtaacc ggtccgattc gcaacatggt gaataccgtc
     9301 gaccgcatcc gtcgcgggca actcgacagc cgggtggaag gatttatgct cggcgagctg
     9361 gatatgctga aaaacggtat caactcgatg gcaatgtcgc tggcggctta tcacgaagag
     9421 atgcaacaca atatcgacca ggcgacgtcc gatctgcgtg agacgctgga gcaaatggag
     9481 attcagaacg ttgagttgga tctggcgaaa aagcgcgccc aggaagcggc gcgtattaaa
     9541 tccgagtttc tggcaaatat gtcacacgag ctgcgtacac cactgaatgg tgttattggc
     9601 tttacccgcc tgacgctgaa aacagaatta acaccaacgc agcgcgatca cctgaatacg
     9661 attgaacgtt cggcaaataa tttgctggca attattaatg atgttctcga cttctcgaaa
     9721 ctggaagccg gtaagctgat tctggaaagt attccattcc cactacgcag cacgctggat
     9781 gaagtcgtta ccctgctggc acattcttct cacgataaag ggctggaact gacgctcaat
     9841 attaaaagcg acgtgcctga taacgtgatc ggcgacccac tacgattaca gcaaatcatc
     9901 actaacctgg tagggaatgc aattaaattc accgagaatg gcaacatcga tattctggta
     9961 gaaaaacgtg cgctgagtaa taccaaagtg cagattgaag tgcagattcg ggataccggc
    10021 attggtattc ctgaacgcga tcaatcgcgc ttattccagg ccttcagaca ggctgatgcc
    10081 agtatttccc gccgtcatgg tggcaccggg ctggggctgg tgattacaca aaaactggtt
    10141 aatgaaatgg gcggcgatat ttcgttccat agcctgccga atcgtggttc aactttctgg
    10201 ttccacatta atctcgatct gaacccgaac attattatcg aagggccatc cacccagtgc
    10261 ctcgctggta agcgcctggc ctatgtcgaa ccaaactccg cagcagcaca atgcacgctg
    10321 gatattttaa gtgaaacgcc gctggaagtg gtttatagcc caacgttctc cgcgctgcct
    10381 cccgcgcatt acgacatgat gttattaggc atagcggtga ccttccgcga gccgctaaca
    10441 atgcaacatg agcgattagc gaaagcagta tcgatgaccg atttcctgat gctggcactt
    10501 ccttgccatg cacaagtcaa tgctgaaaaa cttaagcaag atggtatcgg cgcgtgtctg
    10561 ctgaaaccat taacacctac gcgcctgttg cctgccctga cggaattttg tcatcacaaa
    10621 caaaacacgc ttttgcctgt aaccgatgaa agtaagctgg caatgacagt catggcggtt
    10681 gatgacaacc ccgctaacct gaaacttatc ggcgcattgc tggaagatat ggtgcaacat
    10741 gtggaacttt gcgatagcgg gcatcaggcg gttgaacggg cgaaacagat gccgttcgat
    10801 ttaatcttaa tggatattca aatgccggac atggacggca ttcgggcatg tgagctcatc
    10861 caccagctcc cgcatcagca gcaaacgccg gttatcgcgg taacggcgca tgcgatggcc
    10921 gggcaaaaag agaaactgct tggcgcgggg atgagtgatt atctggccaa accgatcgaa
    10981 gaagagcgat tgcataattt gctgttgcgc tacaagcctg gcagcggtat ttcctctcgc
    11041 gtcgtgacgc ccgaagtcaa cgaaattgtg gtcaacccga atgcgaccct cgactggcaa
    11101 ctggcactac gccaggcagc aggaaaaacc gatttagcgc gcgatatgct gcaaatgtta
    11161 ctcgatttcc tgcctgaagt tcgcaacaaa gttgaggaac agctggttgg agaaaacccg
    11221 gaaggcctgg ttgatttgat tcataaactg catggcagtt gcggctatag cggtgtaccg
    11281 cgtatgaaga atctctgcca actgatcgaa caacagctac gtagcggtac taaagaagaa
    11341 gatttagagc cggagctgct ggaactgttg gacgagatgg ataatgtagc gcgcgaagcc
    11401 agcaaaattc tcgggtaatg gacgtcttgt cgagtagcaa ccggattttt atgacgctgg
    11461 tttcagattg gtgaaaagtg cctgtgttta tgccggatgc ggcgttaacg ccttatccgg
    11521 cctacaaaac caattaaatt caatgaatta aaaaatgatg taaccctgat acgtagcgca
    11581 acaggcagtt tggcatcagt ctcaaaccgg ctccagatag agccggtttt ggttttctgt
    11641 cttaacgcac catgcacggg cgcttgttat caaacgtcca gccggggatc aggtactgca
    11701 ttcccatcgc atcgtctcgc gcaccaagcc catgtttctg atacagctcg tgggctttca
    11761 tcacctgatc catatcgatt tctacgccca atcctggttt ttgcggcacc tgtaccagcc
    11821 cgcctttgat ctcaaacggt tctttggtca gacgctgatt gccttcctgc cagatccagt
    11881 gcgtatcaat agcagtaatt ttacccggtg cagcagcggc tacatgagta aacatcgcca
    11941 gggaaatatc gaagtggttg ttagagtgtg aaccccaggt caggccaaat tcatggcaca
    12001 tttgcgccac acgtaccgaa ccttgcatcg tccagaaatg cggatccgcc agcgggatat
    12061 caacggattg taacgagagc gtatggccca tctgccgcca gtcggtggcg atcatattgg
    12121 ttgcggtcgg caggccagtc gcgcgacgga actctgccat cacttcacgc ccggagaaac
    12181 cttgctccgc accacacgga tcttctgcat aagccagcga acctttcagg tatttaccaa
    12241 ttttaatcgc ttcgttcagc gaccaggcac cgttaggatc gagggtaata cgcgcctgtg
    12301 ggaagcgttg cgccagtgcc acaatagatt ctgcttcttc ttccccggcc agtacaccgc
    12361 ccttcagttt gaaatcgttg aagccatatt tttcatatgc cgcttccgcc aggcgcacca
    12421 ccgcatccgg cgtcatcgct tcttcgtgac gcaggcgata ccagtcacat ttatcatccg
    12481 gctggctttg atacggcagc ggtgtggctt tgcgattacc gacgaagaac agataaccga
    12541 gcatttcgac ttcgctacgc tgttgaccat cgcccagcag cgatgccacg ttaaccccca
    12601 gatgctgccc cagcagatcc agcattgccg cttctatccc ggtaaccaca tgaatagtgg
    12661 tccgcaggtc aaatgtctgc aaaccgcgcc cgccggcatc acgatcggca aatgtattac
    12721 gcaccagcgt cagaacgttt ttgtattcac ccagcgtttt acccaccacc agcggaatcg
    12781 catcttccag cgttttacgg attttctcgc cgccgggaat ttcccctacc ccggtgtgac
    12841 cagaattatc tttgataatc acaatattac gcgtgaagaa cggtgcgtgt gcaccactca
    12901 gattcatcag catactgtca tggcccgcca ccgggatgac ctgcatttca gtaacaacag
    12961 gcgtcgaaaa ttgagaactc atctttatgt cctttttcaa aatcaatgac ggccgaaaac
    13021 gggacgttta cggtcaaagg tccagccggg gatcaggtac tgcatcgggc ccgcgtcgtt
    13081 acgcgcaccc ccaggcagac gtttataggc ctcatgcgcc ttttgtacct gctcccagtc
    13141 cagtcccacg cccagaccgg gcgcatcagg aacggcaatt tttccgtttt taatctccag
    13201 cggatttttg gtcaggcgac aatcgccctc ctgccaaatc cagtgggtat cgatagcggt
    13261 aggattaccc ggtgccgccg cacccacatg ggtaaacatc gccagagaga tatcaaagtg
    13321 gttattggaa tggcagcccc aggtcagccc ccagtcgtcg caaagttgcg ccacgcggac
    13381 tgcaccggaa agcgtccaga agtgcggatc ggcaagtgga atatctaccg cattgagcat
    13441 caccgcatga cccatttcgc gccagttggt ggcgatcatg ttggtcgcga cgggcaagcc
    13501 ggtcgcccgt cgaaactccg ccatcacttc acgaccggag aaaccctgct cagcgccgca
    13561 cgggtcttcc gcataggtaa ggacatcatt cagccctttg cacagagaaa tggcttcatc
    13621 aagcagccag gcaccattgg gatcaacggt aatccgcgca tcagggaagc gtttcttcaa
    13681 tgcacgaaca gtgtcgattt cttgctcgcc aggtaacacg ccacccttaa gtttgaaatc
    13741 tttaaagcca tagcgatcct gtgaggcttc cgccagacgc acaacggctt cgctgttcat
    13801 cgctttctga tgccgcaact gataccactc atggttgccc ggcgtatttt ccagataagg
    13861 aagatcggtt ttggtccgat caccgatata aaacagataa ccgagaacgg taatagcatc
    13921 acgttgcttg cctggcccta acagttcgca gaccggaaca ttcagcgcct gacctaacag
    13981 gtcaagcaag gcggcttcca gcgccgccac ggcattaacg cgcaattcaa aagtccaggc
    14041 acctttgccg aaggtatcaa aatcggctgc ctgattacct ttatgcacct gctgaaccac
    14101 tttattcagg cgcgcaactt cctggcccag caccatcgga atagccttga ccagtgtttg
    14161 ataaatcacc tctccgcccg gcgcttcacc aatgccggta tgcccggcat tatcggtgag
    14221 taccacaata ttgcgggtga aatatgcgtt atgtgcacca ccaatattaa gcaacatgct
    14281 gtcatgtccg gcgaccggaa tgactttcat atcagtaata acaggactgg attgtgtcgt
    14341 catcttattg ccccgcgaca ggtttcagct caatacgctt gatatcgccc accagcacca
    14401 ggtagctgag caccgcgatt aaggcatgaa caccaacata aatcagcgcc ccattaaagg
    14461 agccagtcgt gccaacgata taaccaattg cgattggcgt gacgatgcca gaaatgttac
    14521 caaacatgtt aaacaggcca ccggaaagac cgctgatctc ttttggcgca gtatcagcca
    14581 ttacagccca acccagcgca ccgatcccct taccgaagaa ggccagcgcc ataaagccga
    14641 tgatcatcca ctcaacgttg acgtagttgc agaacaccat caccatcgac aacaacatgc
    14701 ccatcacgat cggtgtttta cgcgcaatgt tcagtgaacc cgtgcgacgc atcagccagt
    14761 cggaaataat cccacccagc acaccgccga taaaaccgca aaccgccgga acggaagcca
    14821 caaagcccgc tttcagaatc gacatcccgc gcgcctgcac cagataaacc gggaaccagg
    14881 taatgaaaaa gtaagtcagg gcgttgatac agtactgacc gatataaacg ccgatcatca
    14941 tccgtgaccc gagcagctgt ttgatctgcc cccacttcac gctgaacggc actttaactt
    15001 tggtgttttg ctgatccata ttgatcagcg cgccacccgc ggcgatgtac tccagctctt
    15061 tctgatttac gcccggatgt tgatttggct cgtggatgac tttcaaccag ataaagctga
    15121 tgacaatgcc cagaccgccc ataaagaaga agacgtgtga ccagcccact tcatgagtta
    15181 gccagcccat aatcggcgcg aagataaccg ttgcgaagta ttgggcggag ttaaaaatcg
    15241 acaccgccgt tcccctttcc tgcgccggaa accaggccgc aacaatgcgg ctgttgccag
    15301 ggaaagatgg cgcttcagca agcccgacca ggaagcgcag tgtaaacagg gcaacgataa
    15361 tgccgaatcc actaaagata tcgacgaagc cttgcagcaa ggtaaacatc gaccagataa
    15421 agatcgacca gaagtagacg cgttttgaac caaaacgatc cagcaaccag ccaccaggga
    15481 tctgcccgat aacatacgcc catgagaagg cagagaacac atagcccatt cccacgggat
    15541 caaggccgat atctttggcc atttccgaac cggcaataga aagcgtagcg cggtcgccgt
    15601 agttgaagga tgtgacgata aacaacatca ccactatcca gtaacgagca ttcgtgcgtt
    15661 tttccacact gctcgcagcc tgacttaaag aactcattgt tgcactcctg aaaattcgcg
    15721 ttagccacgc tcactctgga ctgcgacatc gccaggaaat cagaggtgac gtagggtgtt
    15781 ttttgccgtt tttataggtc gttcgccgaa tacggcgcgt gtttatatct ggcaatagca
    15841 gtataaaaag cgcgccatag cggctcaccg tgcaacaaca caacattaat gcgttcaatg
    15901 aggcccgatt ttggcattag ccctggacgg tggaatccac ttcacggaaa tgaaaacaag
    15961 aacaagaaag gaagggttaa aacgaagaaa taaagaagag tatgaaatgg atcgcttgac
    16021 tccaggcaaa cgccagtaaa aatccgcgct atgaagcagt ttttactggc atttgcctga
    16081 aaagattcga ttcagcaccg ctaaaacggc atttaccgct cgctgaacat atcaggacaa
    16141 catcgtgccc caatgttcga cccacggatt tgattccgtt tccggttccg ggttctcact
    16201 ggcgtcaatc aacagcattt cgccaacccg ctgagcgctc tgttcctgca acaaggcatc
    16261 gaactgtttg ccactattgc agaaattcac ataactactg tcgccgagcg caatcacgcc
    16321 ataacgcaga ttcggctgga agcccagggt atctttaatt ccctgaaaga gcggcacaat
    16381 gctatcagga aggtcgccct gcccggtcgt ggacgtaacc accagaacat acttatcctg
    16441 atagggcagc cagtcgctta attcaggatc ttcaaatacc gttgctttgt ggccctgcgc
    16501 ggtcagaatt gcttccgctt cttccgccac taacagcgaa tttccgtaca tagtgccgac
    16561 aaaaatacca atttccgcca taccgttttc tccctggatt aggaacttat ctctccatcc
    16621 tgacccgatg gcgcactgaa ctcaaccctt tcattttcag gaagcagacc ccgccagcca
    16681 aattgtgata acgcctgcat ccaggtgtcg tccaggcccg cgtgaatagt cagtggctcg
    16741 ccagtaaaag gatgcgtcaa tgacaactga ctggcatgca gcattaaccg ctgaaggcca
    16801 aaatgttcag caccgctgcg attctggcgt aaatcaccat gtttgctgtc gccaataatc
    16861 gggtggcgca aatgggcaag atggcgacgg agctggtgct tacgtccggt tttcggctcc
    16921 agttccacca ggccgtagcg cgtggtcggg taacgtccgg tcgctaccag catttctacg
    16981 gtcgccagac cgcgataatg cgtcactgct ggctgcgggc ctttatcttc gcgggcaaat
    17041 ttatcagcga ttttgtccag ttcttccacc agtggataat ccagcaccgc ttcttccatc
    17101 aaccagccgc gcacaatcgc atggtaacgt ttctggattt ggtgctgttc aaactgttgt
    17161 gccagcagcc gtccggcctc gctggataat cccatcaaca acacaccaga agtgggtcgg
    17221 tccagacgat gagcagtaaa cacatgctgg cctatcaggt cacgcacggt ttgcatgacc
    17281 actactttct cgtcgcgatc cagccagctg cggtgaacca gccagccgga gggtttattt
    17341 accgcaacca gccattcatc ctgatagagt atttccagca ttagctcgca tcatccgcaa
    17401 aaagagcatc cagtttttcc agctcagcca gaataagcgc gcgttgcgga tgatcagtcg
    17461 ccagcgccat ttcataatag ggtgcaacgg caaaagcgcc cggtaacggc tggttgttat
    17521 ctaacaaatc gtgcattcgc gggatcagca cccactgcaa ccactccagt ggttccatgg
    17581 tgtccataaa gaacggttgg gtactgttaa attgatgcgg ctggggttta tcgtttcgcc
    17641 agtgctgatg ttcacgcagt aacgcttcaa gcgcctgcaa ctggagacga acgcggtcat
    17701 gagtggtcat aaagcaacct caataagaaa aactgccgcg aaggatagca ggaataaaaa
    17761 aagggagcac tgtattcaca gtgctcccgg ttcgtttcgc agcattccgg ctacttttgt
    17821 tgctccctgc tcatccttga caacttttcc tctggccttg cggccaatcg ttcatcctga
    17881 actattgctt cctgctcaca ccaccccgat gtgatacttc atcctgaagt gttcctggcc
    17941 ttcctgaccc accgaatcat cctgaccggt tctcattctc catcctggag gtgtccttta
    18001 acgcgtcctg cgtcatcctc ttcgcttcat ccagaagcct ttccctgaaa caccatcctg
    18061 gtgtgtcctt cagaagtgtc atcatcctga tgttcacttc gttgtctgac tccctgtcga
    18121 cgaagataga atcgtctttt tcgcgaagtc ttacaaggcg cttacagaca atgccttaag
    18181 gaaaatttca tatgaaagtt aagaattaac aagcaacatg ttgtttttaa atgagaataa
    18241 cgattcttat ctttttaagt attcgcttat ttcctggcga tctcctacag agtctgtaag
    18301 agatctctca caaccagatg gggtatttgg attacagaag aggcttaagt tgattaagga
    18361 attccgcaag atttgaggct aaatgcgtcc gtttacgtgt tcctagcgtc tctttgcaca
    18421 cttcaccgga gagattacac accgagatga cctctaactc ttcttccagc gtcgcgataa
    18481 atagcgtggg cggcagtttt agccttttct gagtcaccag atgaccaatc agattttcct
    18541 gcacccgacg gaaatcgtct tcactccagg tttgcagcaa tgtcagtttg atatcgccaa
    18601 actgcgcgtg catatccccg gcaaactgag tcgtataaaa ggtatgaatc gtgggttgta
    18661 tcacaatatc aaaagcgcgt tcaaccgcgt ttacattttg ttccccggta aacggctggg
    18721 gttgccagta tacggcatct tcggtagttg aaataatgca cggtgaagga acgccgtaca
    18781 gttcctcact taacggccag cttttatgct cttcatgcca cgcatcgcag tagcgtgcag
    18841 taaaatcttt cagggcttgt gcggtcaaat cgtccactaa tttctctctt cacttaaacc
    18901 agatacactt gtcgtttagt ttatctggtt tatgacggtg aaacatgtct tcttatgcaa
    18961 accatcaggc acttgcgggc ctgactcttg gaaaatcaac cgattaccgg gatacctatg
    19021 acgccagcct actgcaaggc gttccacgca gcctgaatcg cgacccgctg ggtctgaaag
    19081 cggataacct gccttttcac ggtacggata tctggacgct gtatgaactt tcctggctga
    19141 atgcgaaagg tttgccgcag gtcgctgtcg gtcatgttga acttgattac accagcgcaa
    19201 atctgattga gtcgaagagt tttaagctct atctcaacag ttttaaccag acgcgtttta
    19261 ataactggga tgaggtgcgc cagacgctgg agcgcgactt aagcacttgc gctcagggta
    19321 aggttagcgt ggcgttatat cgtcttgatg aactggaagg ccaaccgata ggtcatttca
    19381 atggcacttg cattgatgac caggatatca ctatcgataa ctatgaattc actactgact
    19441 atctggagaa tgccaccagt ggcgaaaaag tagtagaaga gacgctcgtc agccacctgc
    19501 tgaaatcaaa ttgcctgatc acccatcaac cagattgggg atcgatccaa attcagtatc
    19561 gtggacgcca aattgacaga gaaaaactgc tgcgttacct ggtttctttc cgtcatcaca
    19621 acgagttcca cgaacagtgc gtggaacgca tctttaatga cctgttacgc ttctgccagc
    19681 cagagaaatt gagcgtttac gcacgttata cccgtcgtgg cggtctggac attaacccgt
    19741 ggcgcagtaa tagcgatttt gtcccatcga ccacaagact ggttcggcaa taaatttttt
    19801 ctcaattttg cgtgctggat tcacgcagaa ggttgtgaaa ggtcatcagg cagggctatt
    19861 gtaatcaaag ggaatgacga tattcgtccc ataaggagtt tttcttgatt acacatatta
    19921 gcccgcttgg ctccatggat atgttgtcgc agctggaagt ggatatgctt aaacgcaccg
    19981 ccagcagcga cctctatcaa ctgtttcgca actgttcact tgccgtactg aactccggta
    20041 gtttgaccga taacagcaaa gaattgctgt ctcgttttga aaatttcgat attaacgtcc
    20101 tgcgccgtga acgcggcgta aagctggaac tgataaatcc cccggaagag gcttttgtcg
    20161 atgggcgaat tattcgcgct ttgcaggcca acttgttcgc ggttctgcgt gacattctct
    20221 tcgtttacgg gcaaatccat aacaccgttc gttttcccaa cctgaatctc gacaactccg
    20281 tccacatcac taacctggtc ttttccatct tgcgtaacgc tcgcgcactg catgtcggtg
    20341 aagcgccaaa tatggtggtc tgctggggcg ggcactcaat taacgaaaac gagtatttgt
    20401 atgcccgtcg cgtcgggaat cagctgggcc tgcgtgagct gaatatctgc accggctgtg
    20461 gtccgggagc gatggaagcg ccgatgaaag gcgctgcggt cggacacgca cagcagcgtt
    20521 acaaagacag tcgttttatt ggtatgacag agccgtcgat tatcgccgct gaaccgccta
    20581 acccgctggt caacgaattg atcatcatgc cggatatcga aaaacgtctg gaagcgtttg
    20641 tccgtatcgc tcacggtatc attatcttcc ctggcggtgt gggtacggca gaagagttgc
    20701 tgtatttgct gggaatttta atgaacccgg ccaacaaaga tcaggtttta ccattgatcc
    20761 tcaccggccc gaaagagagc gccgactact tccgcgtact ggacgagttt gtcgtgcata
    20821 cattgggtga aaacgcgcgc cgccattacc gcatcatcat tgatgacgcc gctgaagtcg
    20881 ctcgtcagat gaaaaaatcg atgccgctgg tgaaagaaaa tcgccgcgac acaggcgatg
    20941 cctacagctt taactggtca atgcgcattg cgccagattt gcaaatgccg tttgagccgt
    21001 ctcacgagaa tatggctaat ctgaagcttt acccggatca acccgttgaa gtgctggctg
    21061 ccgacctgcg ccgtgcgttc tccggtattg tggcgggtaa cgtaaaagaa gtcggtattc
    21121 gcgccattga agagtttggt ccttataaaa tcaacggcga taaagagatt atgcgtcgta
    21181 tggatgacct gctacagggt tttgttgccc agcatcgtat gaagttgcca ggctcagcct
    21241 acatcccttg ctacgaaatc tgcacgtaat ctccctcccc cggtgacgtt tgttgccggg
    21301 ttttctcgtt tttggtcaca tacccatcaa tttatt
//
LOCUS       DAZFLD010000071        21315 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.071, whole genome shotgun sequence.
ACCESSION   DAZFLD010000071 DAZFLD010000000
VERSION     DAZFLD010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21315
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.071"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            1..170
                     /locus_tag="ACHKBL_002243"
                     /pseudo
     CDS             1..170
                     /locus_tag="ACHKBL_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003844207.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            complement(195..509)
                     /gene="fliE"
                     /locus_tag="ACHKBL_002244"
     CDS             complement(195..509)
                     /gene="fliE"
                     /locus_tag="ACHKBL_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HFO5819110.1"
                     /translation="MSAIQGIEGVISQLQATAMSARTQESLPQPTISFAGQLHAALDR
                     ISDTQTAARTQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSM
                     QV"
     gene            724..2382
                     /gene="fliF"
                     /locus_tag="ACHKBL_002245"
     CDS             724..2382
                     /gene="fliF"
                     /locus_tag="ACHKBL_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HFO5819111.1"
                     /translation="MNATAAQTKSLEWLNRLRANPKIPLIVAGSAAVAVMVALILWAK
                     APDYRTLFSNLSDQDGGAIVSQLTQMNIPYRFSEASGAIEVPADKVHELRLRLAQQGL
                     PKGGAVGFELLDQEKFGISQFSEQVNYQRALEGELSRTIETIGPVKGARVHLAMPKPS
                     LFVREQKSPSASVTVNLLPGRALDEGQISAIVHLVSSAVAGLPPGNVTLVDQGGHLLT
                     QSNTSGRDLNDAQLKYASDVEGRIQRRIEAILSPIVGNGNIHAQVTAQLDFASKEQTE
                     EQYRPNGDESQAALRSRQLNESEQSGSGYPGGVPGALSNQPAPANNAPISTPPANQNN
                     RQQQASTTSNSGPRSTQRNETSNYEVDRTIRHTKMNVGDVQRLSVAVVVNYKTLPDGK
                     PLPLSNEQMKQIEDLTREAMGFSEKRGDSLNVVNSPFNSSEESGGELPFWQQQAFIDQ
                     LLAAGRWLLVLLVAWLLWRKAVRPQLTRRAEAMKAVQQQAQAREEVEDAVEVRLSKDE
                     QLQQRRANQRLGAEVMSQRIREMSDNDPRVVALVIRQWINNDHE"
     gene            2375..3370
                     /gene="fliG"
                     /locus_tag="ACHKBL_002246"
     CDS             2375..3370
                     /gene="fliG"
                     /locus_tag="ACHKBL_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HFO5819112.1"
                     /translation="MSNLTGTDKSVILLMTIGEDRAAEVFKHLSQREVQTLSAAMANV
                     TQISNKQLTDVLAEFEQEAEQFAALNINANDYLRSVLVKALGEERAASLLEDILETRD
                     TASGIETLNFMEPQSAADLIRDEHPQIIATILVHLKRAQAADILALFDERLRHDVMLR
                     IATFGGVQPAALAELTEVLNGLLDGQNLKRSKMGGVRTAAEIINLMKTQQEEAVITAV
                     REFDGELAQKIIDEMFLFENLVDVDDRSIQRLLQEVDSESLLIALKGAEQPLREKFLR
                     NMSQRAADILRDDLANRGPVRLSQVENEQKAILLIVRRLAETGEMVIGSGEDTYV"
     gene            3363..4049
                     /gene="fliH"
                     /locus_tag="ACHKBL_002247"
     CDS             3363..4049
                     /gene="fliH"
                     /locus_tag="ACHKBL_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HFO5819113.1"
                     /translation="MSDNLPWKTWTPDDLAPPQAEFVPMVESEETIIEEAEPSLEQQL
                     AQLQMQAHEQGYQAGVAEGRQQGHKQGYQEGLAQGLEQGLAEAKSQQAPIHARMQQLV
                     SEFQTTLDALDSVIASRLMQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGK
                     PQLRVHPDDLQRVDDMLGATLSLHGWRLWGDPTLHPGGCKVSADEGDLDASVATRWQE
                     LCRLAAPGVV"
     gene            4049..5422
                     /gene="fliI"
                     /locus_tag="ACHKBL_002248"
     CDS             4049..5422
                     /gene="fliI"
                     /locus_tag="ACHKBL_002248"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HFO5819114.1"
                     /translation="MTTRLTRWLTTLDNFEAKMAQLPAVRRYGRLTRATGLVLEATGL
                     QLPLGATCVIERQNGSETHEVESEVVGFNGQRLFLMPLEEVEGVLPGARVYAKNISAE
                     GLQSGKQLPLGSALLGRVLDGSGKPLDGLPSPDTTETGALITPPFNPLQRTPIEHVLD
                     TGVRPINALLTVGRGQRMGLFAGSGVGKSVLLGMMARYTRADVIVVGLIGERGREVKD
                     FIENILGAEGRARSVVIAAPADVSPLLRMQGAAYATRIAEDFRDRGQHVLLIMDSLTR
                     YAMAQREIALAIGEPPATKGYPPSVFAKLPALVERAGNGISGGGSITAFYTVLTEGDD
                     QQDPIADSARAILDGHIVLSRRLAEAGHYPAIDIEASISRAMTALISEQHYARVRTFK
                     QLLSSFQRNRDLVSVGAYAKGSDPMLDKAIALWPQLEGYLQQGIFERADWEASLQGLE
                     RIFPTVS"
     gene            5441..5884
                     /gene="fliJ"
                     /locus_tag="ACHKBL_002249"
     CDS             5441..5884
                     /gene="fliJ"
                     /locus_tag="ACHKBL_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HFO5819115.1"
                     /translation="MTEHGALATLKDLAEKEVEDAARLLGEMRRGCQQAEEQLKMLID
                     YQNEYRNNLNSDMSAGMTSNRWINYQQFIQTLEKAITQHRQQLNQWTQKVDIALNSWR
                     EKKQRLQAWQTLQERQSTAALLAENRLDQKKMDEFAQRAAMRKPE"
     gene            5881..7008
                     /gene="fliK"
                     /locus_tag="ACHKBL_002250"
     CDS             5881..7008
                     /gene="fliK"
                     /locus_tag="ACHKBL_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HFO5819116.1"
                     /translation="MIRIAPLITADVDNATLPGGKASDAAQDFLALLSEALAGETTTD
                     KAAPQLLVATDKPTTKGEPLVSDIVSDAQQADLLIPVDETPPVINDKQSTSTSLTTAQ
                     TITLAAAADNNTAKDEKADDLNEDVTASLSALFAMLPGFDNTPKVTDAPSTVLPTEKP
                     TLFTKLTSAQLTTAQPDDAPGTPAQPLTPLVAEAQSKAEVISTPSSVTAAASPLITPH
                     QTQPLPTVAAPVLSAPLGSHEWQQSLSQHISLFTRQGQQSAELRLHPQDLGEVQISLK
                     VDDNQAQIQMVSPHQHVRAALEAALPVLRTQLAESGIQLGQSNISGESFSGQQQAASQ
                     QQQSQRTANHEPLAGEDDDTLPVPVSLQGRVTGNSGVDIFA"
     gene            7113..7577
                     /gene="fliL"
                     /locus_tag="ACHKBL_002251"
     CDS             7113..7577
                     /gene="fliL"
                     /locus_tag="ACHKBL_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HFO5819117.1"
                     /translation="MTDYAISKKSKRSLWIPILVFITLAACASAGYSYWHSHQVAAED
                     KAQQRVVPSPVFYALDTFTVNLGDADRVLYIGITLRLKDEATRSRLSEYLPEVRSRLL
                     LLFSRQDAAVLATEEGKKNLIAEIKTTLSTPLVAGQPKQDVTDVLYTAFILR"
     gene            7582..8586
                     /gene="fliM"
                     /locus_tag="ACHKBL_002252"
     CDS             7582..8586
                     /gene="fliM"
                     /locus_tag="ACHKBL_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HFO5819118.1"
                     /translation="MGDSILSQAEIDALLNGDSEVKDEPTASVSGESDIRPYDPNTQR
                     RVVRERLQALEIINERFARHFRMGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNL
                     NLIHLKPLRGTGLVVFSPSLVFIAVDNLFGGDGRFPTKVEGREFTHTEQRVINRMLKL
                     ALEGYSDAWKAINPLEVEYVRSEMQVKFTNITTSPNDIVVNTPFHVEIGNLTGEFNIC
                     LPFSMIEPLRELLVNPPLENSRNEDQNWRDNLVRQVQHSQLELVANFADISLRLSQIL
                     KLKPGDVLPIEKPDRIIAHVDGVPVLTSQYGTLNGQYALRIEHLINPILNSLNEEQPK
                     "
     gene            8583..8996
                     /gene="fliN"
                     /locus_tag="ACHKBL_002253"
     CDS             8583..8996
                     /gene="fliN"
                     /locus_tag="ACHKBL_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HFO5819119.1"
                     /translation="MSDMNNPADDNNGAMDDLWAEALSEQKSTSGKSAADAVFQQFGG
                     GDVSGTLQDIDLIMDIPVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILIN
                     GYLIAQGEVVVVADKYGVRITDIITPSERMRRLSR"
     gene            8999..9364
                     /gene="fliO"
                     /locus_tag="ACHKBL_002254"
     CDS             8999..9364
                     /gene="fliO"
                     /locus_tag="ACHKBL_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HFO5819120.1"
                     /translation="MNNHATVQSSAPVSTAPLLQVSGALIAIIALILAAAWLVKRLGF
                     APKRTGVNGLKISASASLGARERVVVVDVEDARLVLGVTAGQINLLHKLPPSAPTEEI
                     PQTDFQSVMKNLLKRSGRS"
     gene            9364..10101
                     /gene="fliP"
                     /locus_tag="ACHKBL_002255"
     CDS             9364..10101
                     /gene="fliP"
                     /locus_tag="ACHKBL_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310714.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HFO5819121.1"
                     /translation="MRRLLSVAPVLLWLVTPLAFAQLTGITSQPLPGGGQSWSLPVQT
                     LVFITSLTFIPAILLMMTSFTRIIIVFGLLRNALGTPSAPPNQVLLGLALFLTFFIMS
                     PVIDKIYVDAYQPFSEEKISMQEALEKGAQPLREFMLRQTREADLGLFARLANTGPLQ
                     GPEAVPMRILLPAYVTSELKTAFQIGFTIFIPFLIIDLVIASVLMALGMMMVPPATIA
                     LPFKLMLFVLVDGWQLLVGSLAQSFYS"
     gene            10111..10380
                     /gene="fliQ"
                     /locus_tag="ACHKBL_002256"
     CDS             10111..10380
                     /gene="fliQ"
                     /locus_tag="ACHKBL_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HFO5819122.1"
                     /translation="MTPESVMMMGTEAMKVALALAAPLLLVALVTGLIISILQAATQI
                     NEMTLSFIPKIIAVFIAIIIAGPWMLNLLLDYVRTLFTNLPYIIG"
     gene            10389..11174
                     /gene="fliR"
                     /locus_tag="ACHKBL_002257"
     CDS             10389..11174
                     /gene="fliR"
                     /locus_tag="ACHKBL_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HFO5819123.1"
                     /translation="MMQVTSDQWLSWLSLYFWPLLRVLALISTAPILSERSVPKRVKL
                     GLAMMITFAIAPSLPANDVPVFSFFALWLAVQQILIGIALGFTMQFAFAAVRTAGEII
                     GLQMGLSFATFVDPASHLNMPVLARIMDMLALLLFLTFNGHLWLISLLVDTFHTLPIG
                     GEPLNSNAFLALTKAGSLIFLNGLMLALPLITLLLTLNLALGLLNRMAPQLSIFVIGF
                     PLTLTVGISLMAALMPLIAPFCEHLFSEMFNLLADIISELPLI"
     gene            11464..12087
                     /gene="rcsA"
                     /locus_tag="ACHKBL_002258"
     CDS             11464..12087
                     /gene="rcsA"
                     /locus_tag="ACHKBL_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
                     /protein_id="HFO5819124.1"
                     /translation="MSTIIMDLCSYTRLGLTGYLLSRGVKKREINDIETVDDLAIACD
                     SQRPSVVFINEDCFIHDASNSQRIKHIINQHPNTLFIVFMAIANVHFDEYLLVRKNLL
                     ISSKSIKPESLDDILGDILKKETTITSFLNMPTLSLSRTESSMLRMWMAGQGTIQISD
                     QMNIKAKTVSSHKGNIKRKIKTHNKQVIYHVVRLTDNVTNGIFVNMR"
     gene            complement(12131..12319)
                     /gene="dsrB"
                     /locus_tag="ACHKBL_002259"
     CDS             complement(12131..12319)
                     /gene="dsrB"
                     /locus_tag="ACHKBL_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DsrB"
                     /protein_id="HFO5819125.1"
                     /translation="MKVNDRVTVKTDGGPRRPGVVLAVEEFSEGTMYLVSLEDYPLGI
                     WFFNEAGHQDGIFVEKAE"
     gene            12482..12709
                     /gene="yodD"
                     /locus_tag="ACHKBL_002260"
     CDS             12482..12709
                     /gene="yodD"
                     /locus_tag="ACHKBL_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416463.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid resistance protein YodD"
                     /protein_id="HFO5819126.1"
                     /translation="MKTAKEYSDTAKREVSVDVDALLAAINEISESEVHRSQNDSEHV
                     SVDGREYHTWRELADAFELDIHDFSVSEVNR"
     gene            13008..13823
                     /gene="yedP"
                     /locus_tag="ACHKBL_002261"
     CDS             13008..13823
                     /gene="yedP"
                     /locus_tag="ACHKBL_002261"
                     /EC_number="3.1.3.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050531 - mannosyl-3-phosphoglycerate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0051479 - mannosylglycerate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate phosphatase-related
                     protein"
                     /protein_id="HFO5819127.1"
                     /translation="MLSIQQPLLVFSDLDGTLLDSHSYDWQPAAPWLSRLREANVPVI
                     LCSSKTSAEMLYLQKMLGLQGLPLIAENGAVIQLAKQWQEIDGFPRIISGISHGEISQ
                     VLNTLREKEHFKFTTFDDVDDATIAEWTGLSRSQAALTQLHEASVTLIWRDSDERMAQ
                     FTARLNELGLQFMQGARFWHVLDASAGKDQAANWIIATYQQLSGKRPTTLGLGDGPND
                     APLLEVMDYAVIVKGLNREGVHLHDEDPARVWRTQREGPEGWREGLDHFFSAR"
     gene            complement(13820..15514)
                     /gene="dgcQ"
                     /locus_tag="ACHKBL_002262"
     CDS             complement(13820..15514)
                     /gene="dgcQ"
                     /locus_tag="ACHKBL_002262"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis regulator diguanylate
                     cyclase DgcQ"
                     /protein_id="HFO5819128.1"
                     /translation="MQHETKMENQSWLKKLARRLGPGHVVNLCFIVVLLFSTLLTWRE
                     VVVLEDAYISSQRNHLENVANALDKHLQYNVDKLIFLRNGMREALVAPLDFTSLRNAV
                     TEFEQHRDEHAWQIELNRRRTLPVNGVSDALVSEGNLLSRENESLDNEITAALEVGYL
                     LRLAHNSSSMVEQAMYVSRAGFYVSTQPTLFTRNVPTRYYGYVTQPWFIGHSQRENRH
                     RAVRWFTSQPEHASNTEPQVTVSVPVDSENYWYGVLGMSIPVRTMQQFLRNAIDKNLD
                     GEYQLYDSKLRFLTSSNPDHPTGNIFDPRELALLAQAMEHDTRGGIRMNSRYVSWERL
                     DHFDGVLVRVHTLSEGVRGDFGSISIALTLLWALFTTMLLLSWYVIRRMVSNMYVLQS
                     SLQWQAWHDTLTRLYNRGALFEKARPLAKLCQTHQHPFSVIQVDLDHFKAINDRFGHQ
                     VGDRVLSHAAGLISSSLRAQDVAGRVGGEEFCVILPGANLTQAAEVAERIRLKLNEKE
                     MLIAKSTTIRISASLGVSSSEETGDYDFEQLQSLADRRLYLAKQAGRNRVFASDNA"
     gene            complement(15685..15867)
                     /gene="yodC"
                     /locus_tag="ACHKBL_002263"
     CDS             complement(15685..15867)
                     /gene="yodC"
                     /locus_tag="ACHKBL_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HFO5819129.1"
                     /translation="MSFMVSEEVTVKEGGPRMIVTGYSSGMVECRWYDGYGVKREAFH
                     ETDLVPGEGSRSAEEV"
     gene            complement(15946..16863)
                     /gene="yedI"
                     /locus_tag="ACHKBL_002264"
     CDS             complement(15946..16863)
                     /gene="yedI"
                     /locus_tag="ACHKBL_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HFO5819130.1"
                     /translation="MLLAGSSLLTLLDDIATLLDDISVMGKLAAKKTAGVLGDDLSLN
                     AQQVSGVRANRELPVVWGVAKGSLINKVILVPLALIISAFIPWAITPLLMIGGAFLCF
                     EGVEKVLHMLEARKHKEDPAQSQQRLEKLAAQDPLKFEKDKIKGAIRTDFILSAEIVA
                     ITLGIVAEAPLLNQVLVLSGIALVVTVGVYGLVGVIVKIDDLGYWLAEKSSALMQALG
                     KGLLIIAPWLMKALSIVGTLAMFLVGGGIVVHGIAPLHHAIEHFAGQQSAVVAMILPT
                     VLNLILGFIIGGIVVLGVKAVAKMRGQAH"
     gene            17036..17956
                     /gene="yedA"
                     /locus_tag="ACHKBL_002265"
     CDS             17036..17956
                     /gene="yedA"
                     /locus_tag="ACHKBL_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
                     /protein_id="HFO5819131.1"
                     /translation="MRFRQLLPLFGALFALYIIWGSTYFVIRIGVESWPPLMMAGVRF
                     LAAGILLLAFLLLRGHKLPPLRPLLNAALIGLLLLAVGNGMVTVAEHQNVPSGIAAVV
                     VATVPLFTLCFSRLFGIKTRKLEWVGIAIGLAGIIMLNSGGNLSGNPWGAILILIGSI
                     SWAFGSVYGSRITLPVGMMAGAIEMLAAGVVLMIASMIAGEKLTALPSLSGFLAVGYL
                     ALFGSIIAINAYMYLIRNVSPALATSYAYVNPVVAVLLGTGLGGETLSKIEWLALGVI
                     VFAVVLVTLGKYLFPAKPVVAPVIQDASSE"
     gene            complement(17945..18415)
                     /gene="vsr"
                     /locus_tag="ACHKBL_002266"
     CDS             complement(17945..18415)
                     /gene="vsr"
                     /locus_tag="ACHKBL_002266"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VSPR family DNA mismatch endonuclease"
                     /protein_id="HFO5819132.1"
                     /translation="MVDVHDKATRSKNMRAIATRDTAIEKRLASLLTGQGLAFRVQDA
                     SLPGRPDFVVDEYRCVIFTHGCFWHHHHCYLFKVPATRTEFWLEKIGKNVERDRRDIS
                     RLQELGWRVLIVWECALRGREKLTDAALTERLEEWICGEGASAQIDTQGIHLLA"
     gene            complement(18396..19814)
                     /gene="dcm"
                     /locus_tag="ACHKBL_002267"
     CDS             complement(18396..19814)
                     /gene="dcm"
                     /locus_tag="ACHKBL_002267"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-cytosine methyltransferase"
                     /protein_id="HFO5819133.1"
                     /translation="MQENISVTDSYSTGNVVQAMLEKLLQIYDVKTLVAQLNGVGENH
                     WSAAILKRALANDSAWHRLSEKEFAHLQTLLPKPPAHHPHYAFRFIDLFAGIGGIRRG
                     FESIGGQCVFTSEWNKHAVRTYKANHYCDPATHHFNEDIRDITLSHKEGVSDEAAAEH
                     IRQHIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDARRP
                     AMFVLENVKNLKSHDQGKTFRIIMQTLDELGYDVADAEDNGPDDPKIIDGKHFLPQHR
                     ERIVLVGFRRDLNLKADFTLRDISECFPAQRVTLAQLLDPMVEAKYILTPVLWKYLYR
                     YAKKHQARGNGFGYGMVYPNNPQSVTRTLSARYYKDGAEILIDRGWDMATGEKDFDDP
                     LNQQHRPRRLTPRECARLMGFEAPGEVKFRIPVSDTQAYRQFGNSVVVPVFAAVAKLL
                     EPKIKQAVALRQQEAQHGRRSR"
     gene            complement(19881..20576)
                     /gene="yedJ"
                     /locus_tag="ACHKBL_002268"
     CDS             complement(19881..20576)
                     /gene="yedJ"
                     /locus_tag="ACHKBL_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HFO5819134.1"
                     /translation="MDLQHWQAQFENWLKNHHQHQDAAHDVCHFRRVWATAQKLAADD
                     DVDMLVILTACYFHDIVSLAKNHPQRQHSSILAAEEARRLLREEFVQFPAEKIEAVCH
                     AIAAHSFSAQIAPLTTEAKIVQDADRLEALGAIGLARVFAVSGALGVALFDGEDPFAQ
                     HRPLDDKRYALDHFQTKLLKLPQTMQTARGKQLAQHNAQFLVEFMAKLSAELAGENEG
                     VDHKVIDAFSPAG"
     gene            complement(20616..20897)
                     /gene="drpB"
                     /locus_tag="ACHKBL_002269"
     CDS             complement(20616..20897)
                     /gene="drpB"
                     /locus_tag="ACHKBL_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DrpB"
                     /protein_id="HFO5819135.1"
                     /translation="MEERLSRSPGGKLALWAFYTWCGYFVWAMARYIWVMSRIPDAPV
                     SGFESDLGSTAGKWLGALVGFLFMALVGALLGSIAWYTRPRPARSRRYE"
BASE COUNT         5139 a         5648 c         5478 g         5050 t
ORIGIN      
        1 tcgtaacaac atccgacgtg gtgatatgtc aaatgagaca ctctgatggt caaagggttt
       61 atcgcgttta cagatcgtgg atggcagaaa actttcagaa tcaggtttcc attttaaacc
      121 agaaattaag tgagtttgtc ccatccatgc cccacgcggt gagatcggat gtaatcaaca
      181 acaggttaca aaacctacac ctgcatgctc atcacttcct gatacgccgc caccagctta
      241 ttacgcacct gaatccccat ttgcattgaa actgaggctt tttgcatatc ggtcatcaca
      301 tcgtttaacg ccacgccggg ttcaccgaga gtgaattttt ctgcctgcgt gcgggcggct
      361 gtttgtgtat cgcttatgcg atcgagcgcg gcgtgcagct gcccggcaaa actaatggtc
      421 ggttgtggca gtgattcctg tgtacgcgca ctcatcgccg tagcctgtaa ctggctgata
      481 accccttcaa tcccctgtat cgctgacatt ttcatctccc ggataatttc tggcagcaaa
      541 gcctaccagt aagtcaataa gacaaaggcg ctaaatagca acaaaaaaac gggtttattg
      601 gcggatagaa aaaaacgaaa gcacaaataa tgggagcgtc aatttttcga gtttgctgac
      661 ccgggagtga gtcttgttcc actttgccaa taacgccgtc cataatcagc cacgaggtgc
      721 gcgatgaatg cgactgcagc ccagacaaaa tctcttgagt ggcttaatcg cctgcgtgcg
      781 aatccgaaaa ttccattgat tgttgccggc tccgcagcag tggcggtcat ggtcgcactg
      841 atcctgtggg cgaaagcccc cgactaccgc acattattca gcaatctttc cgatcaggat
      901 ggtggcgcaa ttgtcagcca actgacgcaa atgaatattc cttaccgctt cagcgaagcc
      961 agcggcgcta ttgaagttcc ggcagataaa gttcacgaac tgcgcctgcg cctggcgcaa
     1021 caaggtttgc ccaaaggcgg cgcggtcggt ttcgaactgc tcgatcagga aaagtttggt
     1081 atcagccagt tcagcgaaca ggtgaattat cagcgggcgc tggaaggcga gctttctcgt
     1141 accatcgaaa ctatcggccc ggtaaaaggg gcgcgcgtac atctggcaat gccgaaaccg
     1201 tctttattcg tccgtgaaca aaaatcccct tctgcatcgg tgacggtaaa tctgttaccc
     1261 ggccgcgcac tcgatgaagg gcaaattagc gccattgtac atctggtttc cagcgcggtt
     1321 gctggtctgc cgccggggaa cgtcacgctg gtggatcagg gtggacatct gttaacccag
     1381 tccaatacca gcgggcgcga tcttaatgac gctcagttga aatatgccag cgatgtcgaa
     1441 ggccggattc agcggcgtat tgaagcgatc ctgtcgccaa ttgttggtaa cggtaatatt
     1501 cacgcccagg tcacggcgca actggacttc gccagtaaag aacaaacaga agaacagtat
     1561 cgccctaacg gtgatgaatc tcaggcggcg cttcgttcac gccagcttaa tgagagcgag
     1621 caaagcggtt ccggttatcc aggcggcgta ccgggggcgt tgtcgaatca accggcacct
     1681 gcgaataacg cgccaatcag cacgcctccg gcaaatcaaa ataaccgcca gcagcaggcg
     1741 agcaccacca gcaatagtgg gccgcgtagc acacagcgta atgaaaccag taactacgaa
     1801 gtcgatcgca ccattcgtca taccaaaatg aacgtgggcg atgtgcaacg tctgtcagtc
     1861 gcggtagtgg tgaattacaa aaccttgcca gacggtaaac cgttgcctct cagcaacgaa
     1921 cagatgaagc aaattgaaga tctgacccgc gaggcgatgg gcttttctga aaaacgcggt
     1981 gactcgctca atgtcgttaa ctcgccgttc aatagtagtg aggaaagcgg cggagaactg
     2041 ccgttctggc aacaacaagc gtttatcgat cagttacttg ctgccggtcg ctggttgctg
     2101 gtactgctgg tggcgtggct gctgtggcgt aaagcggtac gcccgcagct aacacgtcgc
     2161 gctgaggcga tgaaagctgt acagcaacag gcgcaggccc gcgaggaagt ggaagatgcg
     2221 gtggaagtcc gcctgagcaa agacgaacaa ctccaacaac ggcgcgctaa ccaacgtctg
     2281 ggggcagaag tcatgagcca gcgtatccgt gaaatgtctg ataacgatcc gcgcgtggtg
     2341 gcgctggtca ttcgccagtg gataaataac gatcatgagt aacctgacag gcaccgataa
     2401 aagcgtcatc ctgctgatga ccattggcga agaccgggcg gcagaggtgt tcaagcacct
     2461 ctcccagcgc gaagtgcaaa ctctgagcgc tgcaatggcg aacgtcacgc agatctccaa
     2521 caagcagcta accgatgtgc tggcggagtt tgagcaagaa gctgaacagt ttgccgcact
     2581 gaatatcaac gccaacgatt atctgcgttc ggtattggtc aaagctctgg gtgaagaacg
     2641 tgccgccagc ctgctggagg atattctcga aacccgcgat accgccagcg gtattgaaac
     2701 gctcaacttt atggagccgc agagcgccgc cgatctgatt cgcgatgagc atccgcaaat
     2761 tatcgccacc attctggtgc atctgaagcg cgcccaggcc gccgatattc tggcgttgtt
     2821 cgatgaacgt ctgcgccacg acgtgatgtt gcgtatcgcc acctttggcg gcgtgcagcc
     2881 agccgcgctg gcggaactga ccgaagtact gaatggcttg ctcgacggtc agaatctcaa
     2941 gcgcagcaaa atgggcggcg tgagaacggc agccgaaatt atcaacctga tgaaaacgca
     3001 gcaggaagaa gccgttatta ccgccgtgcg tgaattcgac ggcgagctgg cgcagaaaat
     3061 catcgatgag atgttcctgt tcgagaatct ggtggatgtc gatgatcgca gcattcagcg
     3121 tctgttgcag gaagtggatt ccgaatcgct gctgatcgcg ctgaaagggg ccgagcagcc
     3181 actgcgcgag aaattcctgc gcaatatgtc gcagcgtgcc gccgatattc tgcgcgacga
     3241 tctcgccaac cgtggtccgg tgcgtctgtc gcaggtggaa aacgaacaga aagcgattct
     3301 gctgattgtg cgccgccttg ccgaaaccgg cgagatggtg attggcagcg gcgaggatac
     3361 ctatgtctga taatctgccg tggaaaacct ggacgccgga cgatctcgcg ccaccacagg
     3421 cagagtttgt gccaatggtc gagtcggaag aaaccattat tgaagaggcc gaacccagcc
     3481 ttgagcagca actggcgcaa ctgcaaatgc aggcccatga gcaaggttat caggcgggtg
     3541 ttgccgaagg tcgccagcaa ggtcataagc agggctatca ggaaggactg gcccaggggc
     3601 tggaacaagg tctggcagag gcgaagtctc aacaagcgcc aattcatgcc cggatgcagc
     3661 aactggtcag cgaatttcaa actacccttg atgcgcttga tagtgtgatt gcttcgcgcc
     3721 tgatgcagat ggcgctggag gcggcacgtc aggtgatcgg tcagacgcca acggtggata
     3781 actcggcact gatcaaacag atccaacagt tgttgcagca agaaccgtta ttcagcggta
     3841 aaccacagct gcgcgtgcac ccggatgatc tgcaacgtgt tgatgatatg ctcggcgcaa
     3901 ctttaagttt gcatggctgg cgcttgtggg gcgatcccac cctccatcct ggcggctgta
     3961 aagtctccgc cgatgaaggc gatctcgacg ccagtgtcgc cactcgctgg caagaactct
     4021 gccgtctggc agcaccagga gtggtgtaat gaccacgcgc ctgactcgct ggctaaccac
     4081 gctggataac tttgaagcca aaatggcgca gttgcctgcg gtacgtcgct acgggcgatt
     4141 aacccgcgct accgggctgg tgctggaagc caccggatta caattgccgc tcggcgcaac
     4201 ctgtgtcatt gagcgccaga acggcagcga aacgcacgaa gtagaaagcg aagtcgttgg
     4261 ctttaacggt caacggctgt ttttaatgcc gctggaggaa gtcgaaggtg tcctgcccgg
     4321 cgcgcgtgtt tatgccaaaa acatttcggc tgaagggctg caaagcggca agcagttgcc
     4381 gctcggttcg gcgttattag gtcgcgttct ggacggcagc ggtaaaccgc ttgatggcct
     4441 cccctccccc gatacgacgg aaaccggtgc gctgattacc ccgccattta acccgttgca
     4501 acgtacaccg attgaacatg tgctggacac cggcgtgcgc ccaatcaatg ccctgcttac
     4561 cgtcgggcgc gggcagcgta tggggctgtt tgccgggtcc ggcgttggta aaagtgtgct
     4621 gctggggatg atggcacgtt acacccgcgc cgatgtcatt gtcgtgggtt tgattggtga
     4681 acgagggcgc gaagtaaaag attttattga gaatatcctc ggtgccgaag gacgtgcacg
     4741 ctccgtggtg attgccgctc cggcggatgt ttctccgctc ctgcgaatgc agggtgccgc
     4801 ctatgccacg cgcattgccg aagattttcg cgatcgtggt cagcatgtgt tgctgattat
     4861 ggactccctc acccgctacg cgatggccca gcgtgaaatt gcgctggcga ttggcgaacc
     4921 acctgccact aaaggttatc caccgtcggt gtttgccaaa ttaccggcac tggtcgagcg
     4981 tgccggaaat ggcattagcg gcggcggctc gattaccgcg ttttataccg tgctcaccga
     5041 aggcgatgac cagcaggacc ccattgccga ctccgcgcgg gcaatcctcg acggtcacat
     5101 tgtgctgtct cgccgactgg cggaagccgg gcactatccg gctatcgata ttgaagcgtc
     5161 gatcagtcgc gcaatgacgg cgttgatcag tgagcaacat tacgcgcgag tgcgcacctt
     5221 caaacagctg ttgtcgagtt ttcagcgtaa ccgcgatctg gttagcgtcg gcgcgtatgc
     5281 caaaggcagc gatccgatgc tcgacaaagc catcgccctg tggccgcagc tggagggcta
     5341 tttgcaacaa ggcatttttg aacgcgcgga ctgggaagcg tctctccagg ggctggagcg
     5401 tattttcccg acagtatcat aacccaggag ataacggcag atgacagaac atggcgcgct
     5461 ggcgaccctg aaagatctgg cagaaaaaga ggtagaggat gccgcgcgcc tgctgggtga
     5521 aatgcgtcgg ggatgtcagc aggcggaaga acagctcaaa atgctgatcg attatcagaa
     5581 tgaatatcgc aataacctca atagcgatat gagtgccggg atgaccagca accgctggat
     5641 caactatcag cagtttatcc agacgctgga aaaagccatt actcagcatc gccagcaact
     5701 taatcagtgg acgcagaaag tagacattgc cctgaacagc tggcgagaaa agaaacaacg
     5761 tttgcaggct tggcagacac tgcaggaacg gcaatccacg gcggcactgc ttgcagaaaa
     5821 ccgcctcgat cagaaaaaga tggatgagtt cgcccagcgc gccgccatga ggaaacctga
     5881 atgattcgca tagcgccctt gattaccgct gacgttgata acgccacatt gcctggcggc
     5941 aaagccagcg atgctgcaca agattttctc gcgttgttga gcgaagcatt agcaggcgag
     6001 acaacaaccg acaaagcggc cccccagttg ctggtggcaa cagataagcc cacgacaaaa
     6061 ggcgagccgc tggtcagcga tattgtttcc gatgcgcaac aagcagattt actgatccct
     6121 gtggatgaaa caccgcctgt catcaacgac aaacaatcca catcaacatc gttaaccacc
     6181 gctcagacga taacgttggc tgcggcggct gacaacaata cggcaaaaga cgaaaaagcg
     6241 gatgatctga atgaagacgt caccgcaagc ctgagcgccc tttttgcgat gttgccgggt
     6301 tttgacaata cgcccaaagt gactgatgca ccgtcaaccg tgttaccgac agagaaacca
     6361 acgcttttca caaaactgac ttctgcgcaa ctcactacag cacagcctga tgacgccccc
     6421 ggcacaccag ctcagccatt aacaccgctg gtagcagaag cccagagtaa agcggaagtc
     6481 atcagcacgc cttcgtcggt gaccgctgcc gccagcccgc taatcactcc acaccagaca
     6541 cagccactgc ccaccgtcgc cgcgcctgtt ttgagtgcac cgctgggttc tcacgaatgg
     6601 caacaatcat taagccagca tatttcgctg ttcacccgcc aggggcaaca aagtgcagag
     6661 ttgcgtctgc acccacagga tttaggtgaa gtgcaaattt ccctcaaagt ggatgataac
     6721 caggcgcaaa tccagatggt ttcaccgcat cagcacgtac gcgccgccct ggaagcagcg
     6781 ctgccggtac tgcgtacgca gctggccgaa agtggcattc agttagggca aagcaacatc
     6841 agtggcgaaa gctttagtgg tcagcagcag gccgcttccc aacaacagca aagccaacgc
     6901 acagcaaacc atgaacctct ggcgggggaa gacgacgata cgcttccggt tcccgtctct
     6961 ttacaagggc gtgtaacagg caacagcggc gttgatattt tcgcctaacg tcagaggtag
     7021 caccgtaatc cgcgtctttt ccccgctttg ttgcgctcaa gacgcaggat aattagccga
     7081 taagcagtag cgacacagga agaccgcaac acatgactga ttacgcgata agcaagaaaa
     7141 gcaagcgatc gctttggatc ccgattctgg tattcattac cctcgcggcc tgtgccagcg
     7201 caggttacag ctactggcat tcgcatcagg ttgccgctga agacaaagcg cagcaacgcg
     7261 tcgtgccctc cccggtcttc tatgcgctgg atacctttac ggtcaatctg ggcgatgcgg
     7321 atcgcgtact ttatatcggc ataaccctgc gactcaaaga tgaagctacc cgctcgcggc
     7381 tgagtgagta tttgccggaa gtccgtagcc gtttgctgtt actgttttcg cgtcaggatg
     7441 ctgccgtact ggcgacagaa gaaggtaaga aaaacctgat tgccgagatt aaaaccacac
     7501 tttccacccc gcttgttgcc gggcaaccga aacaggatgt caccgacgtg ctgtataccg
     7561 cttttattct gcgataacga catgggcgat agtattcttt ctcaagctga aattgatgcg
     7621 ctgttgaatg gtgacagcga agtcaaagac gaaccgacag ccagtgttag cggcgaaagt
     7681 gacattcgtc cgtacgatcc gaatacccaa cgacgggttg tgcgcgaacg tttgcaggcg
     7741 ctggaaatca ttaatgagcg ctttgcccgc cattttcgta tggggctgtt caacctgctg
     7801 cgccgtagcc cggacattac cgtcggggcc atccgcattc agccgtacca tgaatttgcc
     7861 cgcaacttgc cggtgccgac caacctgaac cttatccacc ttaaaccgct gcgcggcaca
     7921 gggctggtgg tgttctcacc gagtctggtg tttatcgccg tggataacct gtttggcggc
     7981 gatggacgct tcccgaccaa agtggaaggt cgcgagttta cccatacaga acagcgcgtc
     8041 atcaaccgca tgttaaagct ggcgcttgaa ggctatagcg acgcctggaa ggcgattaat
     8101 ccgctggaag ttgagtacgt acgttcggaa atgcaggtga aatttaccaa tatcaccacc
     8161 tcaccgaacg acatcgtggt taacacgccg ttccatgtgg agattggcaa cctgaccggc
     8221 gaatttaata tctgtctgcc attcagcatg atcgagccgc tacgggaatt gttggttaac
     8281 ccgccgctgg aaaactcgcg taatgaagat cagaactggc gcgataacct ggtgcgccag
     8341 gtgcagcatt cacagctgga gctggtcgcc aactttgccg atatatcgct acgcctgtcg
     8401 cagattttaa aactgaaacc cggtgacgtc ctgccgatag aaaaacccga ccgcatcatc
     8461 gcccatgttg acggtgtccc ggtgctgacc agtcagtacg gcaccctcaa cggtcagtat
     8521 gcgttacgga tagaacattt gattaacccg attttaaatt ctctgaacga ggaacagccc
     8581 aaatgagtga catgaataat ccggccgatg acaacaacgg cgcaatggac gatctgtggg
     8641 ctgaagcgtt gagcgaacaa aaatcaacca gcggcaaaag cgctgccgac gcggtgttcc
     8701 agcaatttgg cggtggtgat gtcagcggaa cgttgcagga tatcgacctg attatggata
     8761 ttccggtcaa gctgaccgtc gagctgggcc gcacacgaat gaccatcaaa gagttattgc
     8821 gtctgacgca agggtccgtc gtggcgctgg acggcctggc gggcgaaccg ctggatattc
     8881 tgatcaacgg ttatttaatt gctcagggcg aagttgtcgt agtcgctgat aaatatggcg
     8941 tgcggatcac cgatatcatt actccgtctg agcgaatgcg tcgcctgagc cgttagtgat
     9001 gaataaccat gctactgtgc aatcttccgc gccggtttct actgcgccac tgctgcaggt
     9061 gagcggcgca ctcatcgcca ttattgccct gatcctcgct gctgcctggc tggtaaaacg
     9121 gctgggattt gccccgaaac gcactggcgt taacggtctg aaaattagcg ccagtgcttc
     9181 gctgggcgcg cgtgaacggg tcgtggtggt cgatgtggaa gatgcacggc tggtgctcgg
     9241 cgttaccgca gggcaaatca atctgctgca taaacttccc ccttcagcac caacggaaga
     9301 gataccgcag accgattttc agtcggtcat gaaaaacttg cttaagcgta gcgggagatc
     9361 ctgatgcgtc gtttgttgtc tgtcgcacct gtccttctct ggctggttac acccctcgcc
     9421 ttcgcgcaac tgacgggtat taccagccag ccgctgcctg gcggtggaca aagctggtcg
     9481 ctcccggtgc agacgctggt gttcattacc tcgttgacgt ttattccggc aattttactg
     9541 atgatgacca gtttcacccg catcatcatt gtttttggtt tattgcgtaa cgcactggga
     9601 acaccctccg cgccacctaa ccaggtattg ctggggctgg cactgttttt gacctttttt
     9661 attatgtcac cggtgatcga taaaatttat gtcgatgcgt accagccatt cagcgaagag
     9721 aaaatctcaa tgcaggaggc gctggaaaaa ggggcgcagc cgctgcgtga gtttatgctg
     9781 cgtcagaccc gtgaggcgga tttaggattg tttgccagac tggcaaatac tggcccgttg
     9841 cagggacctg aagccgtgcc gatgcgcatt ttgctcccgg cttacgtgac cagcgagttg
     9901 aaaaccgcat ttcagatagg cttcacgatc ttcattccgt ttttgattat cgacctggtg
     9961 attgccagcg tgttgatggc attggggatg atgatggtcc cccccgccac cattgctctg
    10021 ccctttaaac tgatgctgtt tgtactggtg gatggctggc aattgctggt cggttcgctg
    10081 gcgcagagct tttacagcta gagaggcaaa atgacacctg aatcggtcat gatgatgggg
    10141 actgaagcga tgaaagtcgc gctggcactg gctgccccgc tattgctggt agcattggtc
    10201 acgggcctta tcatcagtat tttgcaggcc gccacgcaga ttaacgaaat gacgctgtcg
    10261 tttattccga aaatcatcgc cgtatttatc gccattatta ttgccggacc gtggatgctc
    10321 aatctgttgc tggattacgt ccgcaccttg ttcactaacc tgccgtatat catcgggtag
    10381 gccgtacaat gatgcaggtg acaagcgatc aatggctttc ctggctaagt ctgtacttct
    10441 ggccgttact gcgcgtgttg gcgctgatct ccaccgcgcc gattctgagc gaacgcagcg
    10501 taccgaaacg ggtaaaactg ggtctggcaa tgatgatcac gttcgctatt gccccatcat
    10561 tacctgccaa cgatgttcct gttttttcgt tctttgctct gtggctggcc gtgcagcaga
    10621 tcctgatcgg cattgcgctt ggttttacca tgcaatttgc ctttgccgcg gtgcgaaccg
    10681 ctggtgaaat tatcggtctg caaatggggc tgtcatttgc gacgtttgtc gatccggcca
    10741 gccatcttaa tatgcccgtt ttagcgcgta tcatggatat gctggcgtta ctgctgttcc
    10801 tgacatttaa cggtcattta tggttgattt cactgctggt cgataccttt cacaccctgc
    10861 caattggtgg cgaaccgttg aacagcaatg cgtttctggc actcaccaaa gcagggagtt
    10921 tgattttcct taacgggctg atgctggcgt taccgctcat tactctgctg ctgaccctga
    10981 atctggcatt aggtttacta aatcgtatgg ccccgcaatt atccattttt gttattggat
    11041 ttccattaac tctgactgtc ggtatctctt taatggcggc attaatgccg ttaattgcgc
    11101 ctttttgcga acatttattc agtgagatgt ttaatttgct ggctgatatt attagtgaat
    11161 tgccattaat ataattccgt aacgtttatc atgctaccct aaggattatc cgaaaaataa
    11221 tacctacgaa catcttccag gatactcctg cggcgaaata tttgttttaa gctcactcac
    11281 atatcgcaac atttacttta ctttaagaca attccaggca aattatacaa cactttacgg
    11341 gatagtaagt ccgcctgaaa aatcgcgaga gtggcgcatt aggtgaccca tgttgttccg
    11401 tttagtcatg atgaaatatt caggtaaggg gaattatcgt tacgcattga gtgagggtat
    11461 gccatgtcaa cgattattat ggatttatgt agttacaccc gactaggttt aaccgggtat
    11521 ctgttgagta gaggggttaa aaaaagagaa atcaacgaca ttgaaaccgt tgatgacctt
    11581 gccatagctt gtgattcaca gcgcccttca gtggtgttta ttaatgagga ctgtttcatc
    11641 cacgatgctt ctaacagtca gcgcatcaag cacatcatta atcaacatcc caatacgtta
    11701 tttatcgttt ttatggcaat tgccaatgtt cattttgatg aatatctatt ggtcagaaaa
    11761 aatttattga tcagttctaa atcgattaaa ccggaatctc tcgatgatat ccttggcgat
    11821 attctgaaaa aagagacaac gataacctcg tttttaaata tgccgacgtt atcattgagc
    11881 cgaaccgaat cgagtatgtt gcgaatgtgg atggcaggtc agggaaccat tcaaatctcc
    11941 gaccaaatga atatcaaagc caagaccgtt tcatcgcata aaggtaatat taaacgtaag
    12001 atcaaaacgc ataataaaca ggttatctac catgtcgtcc gactgacgga taatgtgact
    12061 aatggtattt ttgtcaacat gcgctaacac attctgactg gtggtttccc accagtcagg
    12121 ctgaattaga ttactctgct ttctccacaa agataccgtc ctgatgccct gcttcattaa
    12181 agaaccagat gccgagcggg tagtcttcca gcgaaaccag gtacattgtg ccttcactaa
    12241 actcctcaac tgccagtacc acgccaggac gacgcggacc gccatccgtt ttgactgtta
    12301 cccgatcatt caccttcatc gttttcctcc tgtggctttg tgccagtgta gaacaatttc
    12361 gtttttctgg cagcgccagg cgcgcgcgag tgctgatttt ctcgacggtc tatacttaag
    12421 agatgccagg cggacttaac gactggcggc aacaacagag taacggttgc gaggaaagat
    12481 gatgaaaacc gcaaaagagt acagcgatac cgcaaaacgt gaggtcagcg tcgatgtcga
    12541 tgccctgctg gcggcgatca atgaaattag cgaaagcgaa gttcatcgca gccagaacga
    12601 ttctgaacac gttagtgtcg atggacgtga atatcataca tggcgtgaat tggcggatgc
    12661 cttcgaactg gatattcatg acttcagcgt ctctgaagtg aatcgttgaa tgcacaataa
    12721 aaaaaatccc gaccctgagg gggtcgggat gaaacttgct taagcaagaa gcacttaaaa
    12781 aattcgttac accaggaaat ctgatgtgtt catcacctta tccgcaattt ttttcgctga
    12841 caagaaaata ttcgtgatat gaatgattaa cgctacttat gaatagaaat gtgacgcgcg
    12901 tcaccccttg gtggctctgg cgtcaacggg atcgctccca aaaaaatcat ctttgataat
    12961 ttgtgctgtt ttgagaatca tcctggtaag gggagtactg cgcaaccatg ctttcaattc
    13021 aacaaccact actggttttt agcgatcttg atggcaccct gctggacagt catagttatg
    13081 actggcaacc ggcagccccc tggctcagcc gtttacgcga agcgaatgtt cccgtcattc
    13141 tctgtagcag taaaacttca gcggaaatgc tgtacttgca aaaaatgttg gggctacaag
    13201 gcttaccgct gattgcagag aatggcgcag tgatccagct tgctaagcaa tggcaggaga
    13261 tagacggttt tccacgcatc atctcaggta ttagccatgg cgaaatcagc caggttttaa
    13321 atacgctacg cgagaaagaa cattttaaat tcacgacttt tgatgatgtc gacgatgcaa
    13381 ccatcgccga atggacggga ttaagccgta gccaggcggc gctgacgcag ctgcatgagg
    13441 cgtcggtaac gctaatctgg cgcgacagtg acgagcgtat ggcacaattt accgctcgtc
    13501 tgaacgaact gggcttgcag tttatgcagg gtgcgcgctt ctggcacgtc ctggatgcct
    13561 ctgccggaaa agatcaggct gccaactgga ttatcgcgac ctatcaacaa ttgtcaggca
    13621 aacgcccaac cacacttggc ctgggcgatg ggccaaacga tgcgccctta ctggaggtaa
    13681 tggattacgc ggtgattgtg aaagggctaa accgtgaagg ggtccatctg catgatgagg
    13741 atccggcccg cgtctggcga acgcagcgtg aaggaccgga aggctggcgt gaagggctgg
    13801 accatttttt ctccgcccgt taagcgttat cgctcgcgaa tacccgattg cgcccggcct
    13861 gttttgcgag gtaaagccga cggtcggcca atgactggag ttgttcaaaa tcataatcac
    13921 cggtttcctc gctgctgctt acccccagcg aggcactgat gcgtatcgtc gtactcttgg
    13981 cgatcagcat ctctttttca tttaacttca ggcgaatacg ttctgcgacc tccgcagcct
    14041 gcgtcagatt cgcgcctggc agaatcacac aaaactcctc accaccgacc cgcccggcaa
    14101 cgtcctgcgc acgtaaggaa ctgctaatta atccggcagc atgagaaaga acacggtcgc
    14161 ccacctgatg accaaagcgg tcattaatcg ctttaaaatg gtcaagatcg acctgaatga
    14221 cagaaaatgg atgttggtgc gtctgacaca atttagcgag cggacgggct ttttcgaaca
    14281 gtgcgccacg gttatataaa cgcgttaagg tgtcgtgcca cgcctgccac tgcaacgagc
    14341 tttgcagaac atacatgttg ctgaccatcc ggcgaatcac ataccaggag agtagtaaca
    14401 tggtggtaaa gagcgcccac agcagggtta atgcaatgct gatactgccg aaatcaccac
    14461 gcacgccttc gcttagcgta tggacgcgca ccagcacacc gtcgaaatgg tccagacgtt
    14521 cccagctaac ataacgactg ttcatacgaa tgccaccccg cgtgtcatgt tccattgcct
    14581 gcgccaacaa ggccagttca cgaggatcaa aaatattccc tgttggatgg tcaggattgg
    14641 aagaggtcaa aaatctcagc ttactgtcat agagctgata ctcaccatcg aggtttttat
    14701 cgatggcgtt tcttaaaaat tgctgcatgg tacgcacggg aatactcatc cccagcacgc
    14761 cataccagta gttttcactg tctaccggaa cactgacggt aacctgcggt tcagtattgc
    14821 tggcgtgttc cggttgcgaa gtgaaccagc gtaccgcgcg gtgacgattt tctcgttgcg
    14881 aatggccgat aaaccagggt tgggtgacat agccgtaata acgcgttggt acattgcgcg
    14941 taaacaaggt cggctgcgtc gaaacgtaaa atccggcacg cgagacatac atcgcctgtt
    15001 caaccatcga cgaggagttg tgcgccagtc gcagcaagta accaacttcc agtgcagcgg
    15061 taatttcatt gtcgaggctt tcattttcgc gagacaggag attcccctcg ctgactaatg
    15121 catccgacac gccattgact ggcagggtgc gtcgtcggtt gagttcgatt tgccaggcgt
    15181 gctcgtcgcg atgctgttcg aactcggtta cggcattacg cagagaggtg aaatccagtg
    15241 gcgctacgag agcttcgcgc atgccattac gcaaaaagat cagtttgtcg acgttatact
    15301 gcaaatgctt atcgagcgcg ttggctacgt tttccagatg attacgctgg ctggagatat
    15361 aggcatcttc cagcaccacc acttcacgcc aggtgagcaa ggtggaaaaa agcaatacca
    15421 cgataaagca gagattaacg acatgaccag gccccaggcg gcgtgcgagt tttttcaacc
    15481 agctctggtt ttccattttt gtctcgtgct gcaccctgac gactcccaac ctgctttttt
    15541 atgattctgg cataacccgg cgataacgta ccgcaaaaca gcgataattt tataatggtg
    15601 gcatgataag acgcgactat cattcattgc aggcattggc acaccaggcc ggatgtgatc
    15661 cggcctggtg ttaatgatac cctttcaaac ttcttccgca gaacgactcc cctcccccgg
    15721 aacaagatcg gtttcatgaa aagcttcccg cttgaccccg taaccgtcat accaacgaca
    15781 ctcaaccata ccgctggagt atccagtgac aatcatccgc gggccgccct ctttaaccgt
    15841 aacttcctca ctaaccataa agctcatact cgcctccttt tttcgagtga aacttgttca
    15901 ccttagttga agatggcgaa ttttgcataa tagccagtgc gagtattaat gtgcctgacc
    15961 gcgcattttc gctactgctt tcactcccag caccacgatg ccgccgatga taaaaccaag
    16021 aatcagattt aaaacagtcg gtaatatcat cgccaccact gcactttgct gcccggcgaa
    16081 atgttcaatg gcgtgatgca gcggcgcaat accatgtacc acaatcccgc cgccgacgag
    16141 gaacatcgcc agcgtgccga caatcgataa tgctttcatc agccagggcg cgataatcaa
    16201 taacccttta cctaatgcct gcatcagcgc gctggatttt tccgccagcc aataccccag
    16261 gtcatcaatc ttaacgataa cccctaccag accgtagacg cccacggtca ctaccagcgc
    16321 gatgcctgaa agcaccagca cctgattaag caacggcgcc tctgccacaa tccccagcgt
    16381 gatggcaacg atttccgcag acaaaataaa atcggtacga atcgcccctt taattttgtc
    16441 cttttcaaac ttcagcggat cctgcgccgc cagcttctcc agacgctgct gactctgcgc
    16501 cggatcttct ttatgtttac gcgcctccag catatgcagc actttctcta ccccttcaaa
    16561 gcagagaaac gcgccgccaa tcatcaacag tggcgtaatc gcccacggga taaacgcact
    16621 gatgatcagc gccagcggca ccagaatcac tttattgatc agcgatcctt tcgccacgcc
    16681 ccagaccacg ggaagttccc ggttggcccg cacgcctgaa acttgttgcg cattgagcga
    16741 taagtcatcc cctaatacac cggcggtttt cttcgccgcc agtttgccca tcacggaaat
    16801 atcgtccagc agtgtggcga tatcatcgag caacgtcagt aaactactcc cggccaaaag
    16861 cattccctct catttttatt gttgaagggt gtaagtatga agcaaaaacg gcagctctga
    16921 tacttgcttc acaggtcaac atttttttaa caatcaagtg caattatatc tctcgccagc
    16981 agaatgattt tcacgtttac tatgacccac ctttttattt cagctgtgag ggattatgcg
    17041 tttccgccag ttgttaccgc tttttggcgc gctgtttgcg ttgtatatca tttggggctc
    17101 aacctatttt gtcattcgga ttggcgtgga aagctggccg ccgttaatga tggcgggcgt
    17161 gcgattcctg gcagccggta ttttattgct ggcatttttg ctactgcgcg gacacaaact
    17221 tcccccgcta cgtccgctgc tcaatgccgc gctgattggc ctgttattgc tggctgtcgg
    17281 taatggcatg gtgacggttg ccgaacatca aaatgttcct tccggcatcg ccgccgtagt
    17341 ggttgcaacc gtgcccctct ttaccctgtg cttcagccgc ctgtttggca ttaaaacgcg
    17401 caaactggaa tgggtgggta tcgccattgg gcttgccgga atcatcatgc tcaatagcgg
    17461 tggaaattta agcggcaatc cgtggggcgc gattctgatt ttaatcggct cgattagctg
    17521 ggcgtttggc tcggtttatg gctcgcgcat taccttacct gtagggatga tggcgggtgc
    17581 gattgagatg cttgcggcag gcgtggtgtt aatgattgcg tcgatgattg cgggtgaaaa
    17641 actgacggcg ctcccttccc tttcaggctt ccttgcggtc ggctatctgg cgctgtttgg
    17701 ttcgattatc gccattaacg cttatatgta tttaatccgc aacgtcagtc cggctctcgc
    17761 caccagctac gcctacgtta acccggtggt cgcggtctta ctgggtacgg gactgggtgg
    17821 cgaaacgctg tcgaagattg aatggctggc gctcggcgta attgttttcg cggtggtact
    17881 ggtcacgttg ggaaaatatc tcttcccggc caagcccgtt gtcgcgccag ttattcagga
    17941 tgcatcaagc gagtaaatga atcccctgcg tgtcgatctg cgcgctggca ccttcgccgc
    18001 agatccactc ttccagacgc tcggtaagcg ccgcatccgt aagcttctcg cgcccacgta
    18061 acgcacactc ccagacaatc agtacgcgcc agccgagttc ctgcaagcga ctgatatcgc
    18121 ggcgatcgcg ttcaacattt ttacctatct tctccagcca gaactcggtt cgagtcgcag
    18181 gcactttaaa cagatagcag tgatgatgat gccagaagca gccgtgggta aatatcacgc
    18241 agcgatattc atcaacgaca aaatccgggc gtccgggcag actggcgtcc tgaacgcgaa
    18301 acgcaaggcc ctgcccggtt aacaaactgg cgaggcgctt ctcaatcgcc gtatcacgcg
    18361 tggcaatcgc gcgcatattt ttgctgcggg tggccttatc gtgaacgtcg accatgttgt
    18421 gcctcttgct gacgcaacgc caccgcctgt ttgatttttg gctcaagcag ttttgccacc
    18481 gcggcaaaga ccggcacgac caccgagtta ccgaactggc gataggcctg agtgtccgaa
    18541 accggaatgc ggaatttcac ttctcctggc gcttcaaaac ccattaagcg cgcacattcc
    18601 cgaggcgtta accgacgtgg gcgatgttgc tgattcagcg gatcgtcaaa gtctttttca
    18661 cccgtggcca tatcccagcc gcgatcgatt aaaatttccg cgccatcttt gtaataacga
    18721 gcagacagcg tacgcgtgac gctttgcgga ttgttcggat aaaccattcc ataaccgaag
    18781 ccattaccgc gcgcctgatg ttttttcgca taacgataga ggtacttcca cagcaccggc
    18841 gtcaggatat atttcgcctc gaccatcggg tccagcagct gggccagcgt cactcgctgc
    18901 gcagggaaac attcgctgat atcacgcagg gtaaaatcgg ctttcagatt cagatcgcga
    18961 cgaaaaccca ccagcacgat gcgttcacgg tgctgcggca ggaaatgttt gccatcgatg
    19021 attttcggat cgtccggccc gttatcttct gcatcagcca cgtcatagcc cagttcgtcc
    19081 agcgtctgca tgatgatgcg gaacgtttta ccctggtcgt gacttttcag gtttttgacg
    19141 ttttcgagca caaacatcgc cggacgacgc gcgtcgataa tgcgcaccac atcaaagaac
    19201 agcgtgccct gagtatcgca ggcaaaaccg tgcgcccgcc cgagcgagtt ctttttcgat
    19261 acgccagcca gcgaaaatgg ctgacaagga aaacccgcca gtaatacatc gtgttcagga
    19321 atgtgttgac gaatatgttc cgccgccgcc tcatcactca cgccttcttt atggctgagg
    19381 gtgatgtcgc ggatatcttc attaaaatga tgcgtcgccg ggtcgcaata atgattggct
    19441 ttataagtgc gtaccgcatg tttgttccat tcgctggtaa acacgcactg tccgccaatc
    19501 gattcaaaac cgcgacggat gccgccaatt ccggcaaata gatcgataaa gcgaaacgca
    19561 taatgcggat gatgtgccgg tggtttgggt aacaacgttt gcagatgggc gaactctttc
    19621 tcacttaaac ggtgccatgc cgagtcattc gccagcgcac gttttaaaat tgccgcgctc
    19681 cagtgattct cacccacacc attaagctgc gccaccagcg ttttaacatc ataaatttgc
    19741 agcagtttct ccagcattgc ctgaacgaca ttcccggtgc tgtatgaatc ggttactgat
    19801 atattttcct gcatagattt aaccggccat ctgagatggc cgacaggtta acataattac
    19861 agccatttag ggccacgcgc tcagcccgcg ggtgaaaacg catctatcac cttgtgatcg
    19921 acaccttcat tctcccccgc cagttcggca ctgagcttcg ccataaactc gactaaaaat
    19981 tgcgcgttgt gctgcgccag ctgcttgcct cttgcggttt gcatggtttg cggtagcttc
    20041 agcaacttag tctggaaatg atccagcgcg tagcgtttat catccagcgg gcgatgctgt
    20101 gcgaacggat cttcaccatc aaacagcgcc acgcccaatg cccctgaaac agcaaacacg
    20161 cgcgccaggc caatcgcccc cagggcttcc agccgatccg catcctggac gattttagct
    20221 tccgttgtta agggggcgat ttgcgcgctg aaactgtgag cggcaatggc atgacaaacg
    20281 gcctcgattt tctccgccgg aaattgcacg aactcttcac ggagcaggcg acgcgcctct
    20341 tctgccgcca ggattgaaga atgctgccgt tgcggatgat ttttcgccag gctcacaata
    20401 tcgtgaaaat aacaggcggt taaaatcacc agcatatcga catcgtcatc tgccgccagc
    20461 ttctgtgccg tagcccagac gcggcgaaaa tgacatacgt catgggccgc gtcctggtgc
    20521 tgatgatggt ttttcaacca gttttcgaac tgtgcctgcc agtgttgtaa gtccatacgc
    20581 gctccgttgc caaagagtcg caaccttagc agtttttatt catagcgtct gctacgtgcg
    20641 ggacgaggtc gggtgtacca ggcaatgctg cctaacaatg ccccgaccag agccataaat
    20701 aaaaatccga ccagtgcccc taaccacttt cccgcggtag agcctaaatc gctttcaaat
    20761 cccgacaccg gggcatcagg gatccggctc atcacccaga tgtaacgcgc cattgcccag
    20821 acgaaatagc cacaccatgt atagaaagcc cacaaagcca atttgccgcc agggctgcga
    20881 gagagtctct cttccatctt tgtcgaaccg tattccattt tgagataacc cggaaaattc
    20941 agacttagta ctattatgtg ataaaagtca catttttcca cattggatga gccagtcaag
    21001 actcaatcat tttttgaaca aatagataat ttttgcgcaa cggtagtgaa tacaggaaaa
    21061 atcgttttga gcgattttat ttatctttga tatcggcttt attaattcat tcaatcaata
    21121 tattagcact gattacaatt ataccttttt attattctgt gtcatgatgc ctccgttatt
    21181 agccttttat cgtcttgttt atattttttg ggccggcatg atgccggctt ttttttattc
    21241 cttcattaat gtgcgcgtga tcacaccagc cgtttggcgc aacaatcatt gataccccct
    21301 atgtttccgc tcaac
//
LOCUS       DAZFLD010000072        21235 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.072, whole genome shotgun sequence.
ACCESSION   DAZFLD010000072 DAZFLD010000000
VERSION     DAZFLD010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21235
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.072"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..353
                     /locus_tag="ACHKBL_002270"
     CDS             <1..353
                     /locus_tag="ACHKBL_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFO5819136.1"
                     /translation="LIPQWVTLEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEP
                     DDDDDETWVLFNVMNGNRAEMSPEAAGIAACLMTYSHHACRTECYAMTVHYYRLRDYA
                     LQHPECSAIMRIID"
     gene            369..845
                     /locus_tag="ACHKBL_002271"
     CDS             369..845
                     /locus_tag="ACHKBL_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HFO5819137.1"
                     /translation="MQQLSFLPGEMTPGERSLILRALKTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLIVGGSQVFSFAEHGLL"
     gene            914..1135
                     /locus_tag="ACHKBL_002272"
     CDS             914..1135
                     /locus_tag="ACHKBL_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HFO5819138.1"
                     /translation="MKIITRGEAMRIHQQHPASRLFPFCTGKYRWHGSAEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            1298..1666
                     /locus_tag="ACHKBL_002273"
     CDS             1298..1666
                     /locus_tag="ACHKBL_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HFO5819139.1"
                     /translation="MSDTHHETNYPDDYNNRPWWGLPCTITPCFGARLVQEGNRLYYL
                     ADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSGELNPRHQHTVTLYARGLTCEAD
                     TLGSCGYVYMAVYPTPETKK"
     gene            1756..2133
                     /locus_tag="ACHKBL_002274"
     CDS             1756..2133
                     /locus_tag="ACHKBL_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TA system toxin CbtA family protein"
                     /protein_id="HFO5819140.1"
                     /translation="MNTLPDTHVREASRCPSPVTIWQTLLTRLLDQHYGLTLNDTPFS
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPRFSTSPRSQLINSIDILRARRATG
                     LMTRDNYRTVNNITRGKHPEAKR"
     gene            2130..2618
                     /locus_tag="ACHKBL_002275"
     CDS             2130..2618
                     /locus_tag="ACHKBL_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
                     /protein_id="HFO5819141.1"
                     /translation="MKLSLTLEADSINVRALNMGRIVVDVDGVELAELINVVCDNGHS
                     LRVVDESDRASTDCTPPFAALTGIRCSTAHITAKDSAWLYSLSHQTNDFGESEWIHFT
                     GSGYLLRTDAWSYPVLRLKRLGLSKTFRRLVVTLIRRYGVSLIHLDASAECLPGLPTF
                     NW"
     gene            2638..2835
                     /locus_tag="ACHKBL_002276"
     CDS             2638..2835
                     /locus_tag="ACHKBL_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HFO5819142.1"
                     /translation="MESLTTETALDILIAWLQNNIDCESGIIFDNDEDKTDSAALLPC
                     IEQAREDIRTLRQLQFLQQNR"
     gene            2920..3786
                     /locus_tag="ACHKBL_002277"
     CDS             2920..3786
                     /locus_tag="ACHKBL_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HFO5819143.1"
                     /translation="MSDITASRPEVVTGHTDVICSTSIRHILAVRKSTLLQIDTLIRQ
                     LAEISAMTESIGGKTALDWAMKQDFRCGCWLMEKPETAMKAITRNLDREIWRDLMQRS
                     GMQSLMDAQTRDTWYRSLEYDNFPEISEANILSTFEQLHRSKDEVFERGVINVFKGLS
                     WDYKTNSPCKFGSKIIVNNLVRWDQWGFHLISGMQADRLADLERMLHLLSGKPIPDNR
                     GNITINLDDHIQSVQGKEDYEDEMFSIKYFKKGSVHITFRKPELVDRLNDIIARHYPE
                     ALASTVNKSRKA"
     gene            3858..4121
                     /locus_tag="ACHKBL_002278"
     CDS             3858..4121
                     /locus_tag="ACHKBL_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001143297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051983 - regulation of chromosome
                     segregation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HFO5819144.1"
                     /translation="MPTSVSLSPYFETFIREQIESGRYNNTSEVIRAGLRALEEREQQ
                     IKLESLQSAVTAGINSGESKSAEEVFGRLTHKYKKMAEGEQPI"
     gene            4118..4444
                     /locus_tag="ACHKBL_002279"
     CDS             4118..4444
                     /locus_tag="ACHKBL_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000779484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO5819145.1"
                     /translation="MKLTVSPLAEHDLEAIGDWIAQDNPVRAISFTEELYQQCLLIAE
                     SPVLYRERPELGAGIRGCSYGRYLLLFRVLDTEVRIERIVHGSRDINRVLAEPDDKPS
                     DHFWQP"
     gene            complement(4858..5394)
                     /gene="yghD"
                     /locus_tag="ACHKBL_002280"
     CDS             complement(4858..5394)
                     /gene="yghD"
                     /locus_tag="ACHKBL_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GspM family type II secretion system protein
                     YghD"
                     /protein_id="HFO5819146.1"
                     /translation="MLRDKFIHYFQQWRERQLSRGEHWLTQHLAGRSPREKGMLLAAV
                     VFLFSVGYYVLIWQPLSERIEQQETMLQQLVAMNTRLKNAAPDIIAARKSATTTPAQV
                     SRVISDSASAHSVVIKRIAERGENIQVWIEPVVFNDLLKWLNALDEKYALRVTQIDVS
                     AAEKTGMVNVQRLEFGRG"
     gene            complement(5396..6574)
                     /gene="gspL"
                     /locus_tag="ACHKBL_002281"
     CDS             complement(5396..6574)
                     /gene="gspL"
                     /locus_tag="ACHKBL_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000097227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspL"
                     /protein_id="HFO5819147.1"
                     /translation="MSSMLEIFIPLCASDPIRWQHRTTDVEHGIWSDVDNEQLQQWLQ
                     TDAIRLYIPGEWISVWQVELPDVARKQIPTILPALLEEELNQDIDELHFAPLKIDQQL
                     ATVAVIHQQHMRNIAQWLQANGITRATVAPDWMSIPCGFMAGDAQRVICRIDECRGWS
                     AGLALAPVMFRAQLNEQDLPLSLTVVGIAPEELSAWAGPDAERLTVTALPAITTYGEP
                     EGNLLTGPWQPRVSYRKQWARWRVMILPILLILVALAVERGVTLWSVSEQVAQSRTQA
                     EKQFLTLFPEQKRIVNLRSQVTMALKKYRPQADDTRLLAELSAIASTLKSASLTDIEM
                     RGFTFDQKRQTLHLQLRAANFASFDKLRSALAADYVVQQDALQKEGDAVSGGVTLRRK
                     "
     gene            complement(6571..7548)
                     /gene="gspK"
                     /locus_tag="ACHKBL_002282"
     CDS             complement(6571..7548)
                     /gene="gspK"
                     /locus_tag="ACHKBL_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001554494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspK"
                     /protein_id="HFO5819148.1"
                     /translation="MITSPPKRGMALVVVLVLLAVMMLVTITLSGRMQQQLGRTRSQQ
                     EYQQALWYSASAESLALSALSLSLKNEKRVHLAQPWASGPRFFPLPQGQIAVTLRDAQ
                     ACFNLNALAQPTTASRPLAVQQLIALITRLDVPAYRAELIAESLWEFIDEDRSVQTRL
                     GREDSEYLARSVPFYAANQPLADISEMRVVQGMDAGLYQKLKPLVCALPMTRQQININ
                     TLDVAQSVILEALFDPWLSPVQARALLQQRPAKGWEDVDQFLAQPLLADVDERTKKQL
                     KTVLSVDSNYFWLRSDITVNEIELTMNSLIVRMGPQHFSVLWHQTGESE"
     gene            complement(7545..8150)
                     /gene="gspJ"
                     /locus_tag="ACHKBL_002283"
     CDS             complement(7545..8150)
                     /gene="gspJ"
                     /locus_tag="ACHKBL_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspJ"
                     /protein_id="HFO5819149.1"
                     /translation="MRRTCAGFTLLEMLVAIAIFASLALMAQQVTNGVTRVNSAVAGH
                     DQKLNLMQQTMSFLNHDLTQMMPRPVRGDQGQREPALLAGAGVLASESEGMRFVRGGV
                     VNPLMRLPRSNLLTVGYRIHGGYLERLAWPLTDAAGSVKPTTQKLIPADSLRLQFYDG
                     TRWQESWSSVQAIPVAVRMTLHSPQWGEIERIWLLRGPQLS"
     gene            complement(8147..8518)
                     /gene="gspI"
                     /locus_tag="ACHKBL_002284"
     CDS             complement(8147..8518)
                     /gene="gspI"
                     /locus_tag="ACHKBL_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001709020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspI"
                     /protein_id="HFO5819150.1"
                     /translation="MKRGFTLLEVMLALAIFALAAMAVLQIASGALSNQQILEEKTVA
                     GWVAENQTALLYLMTREQRAVRHQGESDMAGSRWYWRTTPLSTGNALLQAVDIEVSRH
                     EDFSSVIQSRRAWFSAVGGQQ"
     gene            complement(8515..9078)
                     /gene="gspH"
                     /locus_tag="ACHKBL_002285"
     CDS             complement(8515..9078)
                     /gene="gspH"
                     /locus_tag="ACHKBL_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001394829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspH"
                     /protein_id="HFO5819151.1"
                     /translation="MPERGFTLLEIMLVIFLIGLASAGVVQTFATASEPPAKKAAQAF
                     LTRFAQFKDRAVIEGKTLGVLIDPPGYQFMQRRHGQWLPVSATRLSAQVTVPKQVQML
                     LQPGSDIWQKEYALELQRRRLTLHDIELELQKEAKKKTPQIRFSPFEPATPFTLRFYA
                     SAQNACWAVKLAHDGVLSLNQCDEGMP"
     gene            complement(9082..9537)
                     /gene="gspG"
                     /locus_tag="ACHKBL_002286"
     CDS             complement(9082..9537)
                     /gene="gspG"
                     /locus_tag="ACHKBL_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003912123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HFO5819152.1"
                     /translation="MNSLSRTQKPRAGFTLLEVMVVIVILGVLASLVVPNLLGNKEKA
                     DRQKAISDIVALENALDMYRLDNGRYPTTEQGLEALIQQPANMADSRNYRTGGYIKRL
                     PKDPWGNDYQYLSPGEKGLFDVYTLGADGQENGEGAGADIGNWNLQEFQ"
     gene            complement(9554..10777)
                     /gene="gspF"
                     /locus_tag="ACHKBL_002287"
     CDS             complement(9554..10777)
                     /gene="gspF"
                     /locus_tag="ACHKBL_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001173399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system inner membrane protein
                     GspF"
                     /protein_id="HFO5819153.1"
                     /translation="MALFYYQALERNGRKTKGMIEADSARHARQLLRGKELIPVHIEA
                     RLNASTGGMLQRRRHAHRRVAAADLALFTRQLATLVQAAMPLETCLQAVSEQSEKLHV
                     KSLGMALRSRIQEGYTLSDSLREHPRVFDSLFCSMVAAGEKSGHLDVVLNRLADYTEQ
                     RQRLKSRLLQAMLYPLVLLVVATGVVTILLTAVVPKIIEQFDHLGHALPASTRTLIAM
                     SDTLQTSGVYWLAGLLGLLVLGQRLLKNPAMRLRWDKTLLRLPVTGRVARGLNTARFS
                     RTLSILTASSVPLLEGIQTAAAVSANRYVEQQLLLAADRVREGSSLRAALAELRLFPP
                     MMLYMIASGEQSGELETMLEQAAVNQEREFDTQVGLALGLFEPALVVMMAGVVLFIVI
                     AILEPMLQLNNMVGM"
     gene            complement(10777..12270)
                     /gene="gspE"
                     /locus_tag="ACHKBL_002288"
     CDS             complement(10777..12270)
                     /gene="gspE"
                     /locus_tag="ACHKBL_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001525635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
                     /protein_id="HFO5819154.1"
                     /translation="MVPVAQETTANTVRLPYSFSRRFSLVAWCEASLEILHVHPLSLS
                     VLQELQRGLNAPFTLRQIDEAEFEQRLNAVWQRDSSEARQLMEDLGSAEDFFTLAEEL
                     PETEDLLESDDDAPIIKLINAMLAEAIKEGASDIHIETFEKSLVIRFRVDGTLHEMLR
                     PGRKLASLLVSRIKVMARLDIAEKRVPQDGRIALLLGGRAIDVRVSTMPSAWGERVVL
                     RLLDKNQARLTLERLGLSQQLTVQLRQLLHKPHGIFLVTGPTGSGKSTTLYAGLQELN
                     NHSRNILTVEDPIEYMIEGIGQTQVNTRVGMTFARGLRAILRQDPDVVMVGEIRDTET
                     AEIAVQASLTGHLVLSTLHTNTAVGAITRLQDMGVEPFLLSSSLTGVMAQRLVRTLCP
                     DCRQPAPATDEEKRLLGITDARTVTLYHPQGCPACNHKGFRGRTAIHELIVVDATLRD
                     LIHRQAGELELERYVRQHSAGIRSNGIEKVLAGETSLDEVLRVTMEA"
     gene            complement(12270..14330)
                     /gene="gspD"
                     /locus_tag="ACHKBL_002289"
     CDS             complement(12270..14330)
                     /gene="gspD"
                     /locus_tag="ACHKBL_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020234996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system secretin GspD"
                     /protein_id="HFO5819155.1"
                     /translation="MFWRDMTLSIWRKKTTGLKTKKRLLPLMLAAALCSSPVWAEEAT
                     FTANFKDTDLKSFIETVGANLNKTIIMGPGVQGKVSIRTMTPLNERQYYQLFLNLLEA
                     QGYAVVPMENDVLKVVKSSAAKVEPLPLVGEGSDNYAGDEMVTKVVPVRNVSVRELAP
                     ILRQMIDSAGSGNVVNYDPSNVIMLTGRASVVERLTEVIQRVDHAGNRTEEVIPLDNA
                     SASEIARVLESLTKNSGENQPATLKSQIVADERTNSVIVSGDPATRDKMRRLIRRLDS
                     EMERSGNSQVFYLKYSKAEDLVDVLKQVSGTLTAAKEEAEGTVGSGREVVSIAASKHS
                     NALIVTAPQDIMQSLQSVIEQLDIRRAQVHVEALIVEVAEGSNINFGVQWASKDAGLM
                     QFANGTQIPIGTLGAAISQAKPQKGSTVISENGATTINPDTNGDLSTLAQLLSGFSGT
                     AVGVVKGDWMALVQAVKNDSSSNVLSTPSITTLDNQEAFFMVGQDVPVLTGSTVGSNN
                     SNPFNTVERKKVGIMLKVTPQINEGNAVQMVIEQEVSKVEGQTSLDVVFGERKLKTTV
                     LANDGELIVLGGLMDDQAGESVAKVPLLGDIPLIGNLFKSTADKKEKRNLMVFIRPTI
                     LRDGMAADGVSQRKYNYMRAEQIYRDDQGLSLMPHTAQPVLPAQNQALPPEVRAFLNA
                     GRTR"
     gene            complement(14360..15319)
                     /gene="gspC"
                     /locus_tag="ACHKBL_002290"
     CDS             complement(14360..15319)
                     /gene="gspC"
                     /locus_tag="ACHKBL_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspC"
                     /protein_id="HFO5819156.1"
                     /translation="MARVVFRDARIYLIQWLTKIRHILSQRQSLNTDKEHLRKMVRGM
                     FWLMLLIISAKMAYSLWRYFSFSAEYTVVSSSANKPLRADAKPFDKNDVQLVSQQNWF
                     GKYQPVAAPVKQPESAPVAETRLNVVLRGIAFGARPGAVIEEGGKQQVYLQGERLGSH
                     NAVIEEINRDHVMLRYQGKMERLSLAEEERPPVAVTSKKAVSDEAKQAVAEPAASAPV
                     EIPAAVRQALAKDPQKIFNYIQLTPVRKEGIVGYAVKPGADRSLFDASGFREGDIAIA
                     LNQQDFTDPRAMIALMRQLPSMDSIQLTVLRKGARYDISIALR"
     gene            complement(15337..15747)
                     /gene="gspS2"
                     /locus_tag="ACHKBL_002291"
     CDS             complement(15337..15747)
                     /gene="gspS2"
                     /locus_tag="ACHKBL_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system pilot lipoprotein
                     GspS-beta"
                     /protein_id="HFO5819157.1"
                     /translation="MSIKQMPGRVLISLLLSVTGLLSGCASHNENASLLAKKQAQNIS
                     QNLPIKSAGYTLVLAQSSGTTVKMTIISESGTQTTQTPDAFLTSYQRQMCADPTVKLM
                     ITEGINYSITINDTRTGNQYQRKLDRTTCGIVKA"
     gene            complement(15813..16622)
                     /gene="pppA"
                     /locus_tag="ACHKBL_002292"
     CDS             complement(15813..16622)
                     /gene="pppA"
                     /locus_tag="ACHKBL_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417446.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase PppA"
                     /protein_id="HFO5819158.1"
                     /translation="MFFDVFQQYPAAMPVLATVGGLIIGSFLNVVIWRYPIMLRQQMA
                     EFHGEMPSTQSKISLALPRSHCPHCQQTIRVRDNIPLLSWLMLKGRCRDCQAKISKRY
                     PLVELLTALAFLLASLVWPESGWALAVMILSAWLIAASVIDLDNQWLPDVFTQGVLWT
                     GLIAAWAQQSPLTLQDAVTGVLVGFITFYSLRWIAGIVLRKEALGMGDVLLFAALGGW
                     VGALSLPNVALIASCCGLIYAVITKRGSTTLPFGPCLSLGGIATLYLKALF"
     gene            complement(16752..21235)
                     /gene="sslE"
                     /locus_tag="ACHKBL_002293"
                     /pseudo
     CDS             complement(16752..21235)
                     /gene="sslE"
                     /locus_tag="ACHKBL_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026189.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein metalloprotease SslE"
BASE COUNT         4820 a         6000 c         5363 g         5052 t
ORIGIN      
        1 gcctcattcc acagtgggtg accctggagc cccgtgtctt cggctggatg gaccgtttgt
       61 gcgaagacta ctgcggtggt atctggaatc tgtacaccct gaacaacggc ggcgcattta
      121 tggcccctga accggatgac gatgatgacg aaacatgggt gctgttcaat gtcatgaacg
      181 gtaaccgcgc tgaaatgagt ccggaagccg ccggcattgc ggcctgtctg atgacgtaca
      241 gtcatcacgc ctgtcgtacg gagtgttatg ccatgacggt ccactattac cggctgcggg
      301 attacgccct gcagcatccg gaatgcagcg ccattatgcg catcattgac tgaatggagg
      361 cagggacaat gcagcagctt tcctttctgc ccggagagat gacgcccggc gagcgcagtc
      421 tcattctgcg ggccctgaaa accctggacc gccatcttca tgaacccggc gtggccttca
      481 cctccacccg tgcggcacgg gaatggctga ttctgaacat ggcaggactg gagcgtgaag
      541 agttccgggt gctgtatctg aacaaccaga atcagttgat tgccggtgaa accctcttca
      601 caggcaccat caaccgcaca gaagtccatc cccgggaggt gattaaacgc gccctgtacc
      661 acaatgccgc cgccgtggtg ctggcgcaca atcacccgtc cggtgaagtc acccccagta
      721 aggcagaccg ccttatcacc gaacgtctgg tacaggcact gggcctggtg gatatccggg
      781 tgccggacca tctgatagtc ggtggcagcc aggttttctc ctttgccgaa catggtctgc
      841 tttaacccgt caccgccaca atcacatcac acctgttttc acttttatct tctgtcttca
      901 gaggtatccc attatgaaaa ttatcacccg tggtgaagcc atgcgtattc accaacagca
      961 tcctgcatcc cgtctatttc cgttctgcac cggtaagtac cgctggcacg gcagtgctga
     1021 ggcgtatacc ggtcgtgaag tgcaggatat tcccggtgtg ctggccgtgt ttgctgaacg
     1081 ccgtaaggac agttttggcc cgtatgtccg gctgatgagc gtcactctga actaatgttc
     1141 cgtcacccgg aaaacgagta cgaccagatg acctcagacg ccgctgtgga tgaggccctt
     1201 ctcatcaatg aatacatctt caccgaagcc ggtctccaca ccggctgacc tgtcatcgtt
     1261 aaccccttct taaccgattt tctgtgagga ctttatcgtg tcagacacgc accatgagac
     1321 aaattatccc gacgactata acaaccgccc ctggtggggg ctgccctgca ccataacacc
     1381 ctgttttggc gcccgtctgg tgcaggaggg taaccggttg tattaccttg ccgaccgcgc
     1441 cggtatcaga ggccggttca gcgacgcgga tgcgtaccat ctggaccagg cctttccgct
     1501 gctgatgaaa caactggaac tcatgctcac cagcggtgaa ctgaatcccc gccatcagca
     1561 taccgtcacg ctgtatgcca gaggactgac ctgcgaagcc gacaccctcg gctcctgtgg
     1621 ttacgtttat atggctgttt atccgacgcc cgaaacgaaa aagtaaatca tcagaataac
     1681 cttctgctcc ggcctggtgt tttcaccacg ccactttccc atttttcatc tctgcatatc
     1741 aggaaaatct tcagtatgaa cacattaccc gacacacacg tacgggaggc atcgcgctgc
     1801 ccatctccag taaccatctg gcagacactg ctcacccgac tgctggacca gcactacggc
     1861 ctcacgctaa atgacacacc gttctccgat gaacgtgtga ttgagcagca tattgaggca
     1921 ggcatttcac tgtgtgacgc ggtgaacttt ctcgttgaaa aatacgcgct ggtgcgtacc
     1981 gaccagcccc ggttcagcac tagtccccgc tctcagttaa taaacagtat tgatatcctc
     2041 cgggctcgcc gggcgaccgg cctgatgacc cgcgacaatt acagaacggt aaataacatt
     2101 acccggggta agcatccgga ggcgaaacga tgaaactttc cctgacgctg gaagccgaca
     2161 gcattaacgt gcgggcgctg aacatggggc gcattgtcgt tgacgtcgat ggtgttgagc
     2221 ttgctgaact gattaacgtg gtctgcgata acggtcactc ccttcgtgtt gttgatgaat
     2281 ctgaccgggc ctcaacagac tgcacgccac catttgctgc cctgaccggc atacgctgca
     2341 gtaccgcgca tatcacggca aaagacagcg cctggctgta ctcgctgtca catcagacga
     2401 atgacttcgg cgaatcagaa tggattcatt ttaccgggtc cggttatctg ttacgtactg
     2461 atgcgtggtc gtacccggtt ctgcgactta aacgcctggg cctgtcaaaa acgtttcgtc
     2521 gtctggtcgt aacgctcatc cgccgttatg gcgtcagtct cattcatctg gatgccagcg
     2581 ctgaatgtct gccgggttta cccaccttca actggtaacc gggacaaaag acacagtatg
     2641 gaatcattaa ccacagaaac ggcgctggat attctgattg cgtggctaca gaacaatatc
     2701 gactgcgaat ccggaattat cttcgacaac gatgaggata aaacggattc ggcagcactg
     2761 ctgccctgca ttgaacaggc cagggaagat atccgtacac tgcgccaact gcagtttctg
     2821 caacagaacc ggtgagtctt actcaccaga cttcattcca ctgacgccag cctgaatacg
     2881 gctggcgttt tcgtttataa accaaaagga aaaacgatga tgtcagatat cacagcctcc
     2941 cgtccggaag tggtcaccgg gcatacggac gttatttgct caaccagtat ccgccacatt
     3001 ctggctgtac gaaagagtac actgctgcaa atcgacacac ttatccggca actggctgaa
     3061 atctcagcaa tgacagaaag tattggcggt aaaaccgcac tggactgggc gatgaaacag
     3121 gattttcgct gcggctgctg gctgatggag aaaccggaaa ccgcaatgaa agccatcaca
     3181 cgcaatctcg atcgcgaaat ctggcgtgac ctgatgcaac gttccgggat gcagagtctg
     3241 atggatgcgc agacccggga cacatggtac aggtcactgg aatatgataa ttttccggaa
     3301 atcagtgaag cgaacattct gagcacattt gaacaactgc accggagtaa ggatgaagtg
     3361 tttgagcggg gagtgatcaa cgtcttcaaa gggctgagct gggattacaa aaccaactca
     3421 ccctgtaaat ttggcagtaa aattatcgtc aacaatctgg tgaggtggga ccagtgggga
     3481 tttcatctta tcagtggaat gcaggcagat cgcctggctg acctggaaag aatgttgcat
     3541 ctgctcagcg gtaaaccgat ccccgacaac cgggggaata tcaccattaa tctggatgac
     3601 cacatacagt ctgttcaggg taaagaggac tatgaagacg agatgttcag tattaaatac
     3661 tttaagaaag gctcagtaca catcacgttc aggaaaccag aactggttga caggcttaat
     3721 gatattattg ccagacacta cccggaagcc ctggcaagca cagtgaacaa aagccgcaag
     3781 gcataaaaga ggtattttac aattcgcgaa tattaactat tattaattga tagcaatcaa
     3841 tcggcgagaa aatcaccatg cctaccagtg tatccctcag tccttacttt gaaacgttta
     3901 tccgcgaaca aatagaaagt ggtcggtata acaatacaag tgaagtcatt cgtgccggac
     3961 ttcgtgctct ggaagaacgg gaacagcaga taaagcttga atcactccag agcgccgtta
     4021 ccgcaggcat caacagcggt gaaagtaaaa gtgctgaaga ggtattcggg cgactgacgc
     4081 acaaatacaa aaagatggca gaaggtgaac agccaatatg aaactgactg tttcaccatt
     4141 agcagaacac gatcttgaag ctataggtga ctggatcgct caggataatc ctgtccgtgc
     4201 aatcagtttt accgaagagc tttaccaaca atgtctgttg atagccgaat cgccagtact
     4261 ctacagagaa agacccgaat taggggctgg gatcagaggt tgttcgtatg gtcgttacct
     4321 tctgctgttc agggtactgg atactgaagt tcgtatcgaa cgtatcgttc atggttcccg
     4381 ggatatcaac agggtacttg cagagccaga cgacaaacca tcagatcatt tctggcagcc
     4441 ataaacaaaa tctgtgcgtt aacgctccct aacacaggta gccaggtatt ttataaatct
     4501 ctgtattttg ttgttacttc gattccgcaa ttcagcatat cgaaccagac aaaatgatta
     4561 cagcaaagca caaaaaagag ggattatagc gtcaaaagaa ttcgaattgc acatgggggc
     4621 ctcattggtg gcctcaaaaa caacaaagac aaaacaatcg atacaaaaca attggttata
     4681 taaccagttt cattccgagt ccgggcacca ctattctaaa gaacccgctc acaaggcggg
     4741 tttttgcttt tggatctgac aataaccttc acgaaacaaa ttagcttata aagcctgggg
     4801 gaattactct cgccacgtta acgagagtaa ttttattgat attaatctcc tgatacttta
     4861 tccccgtcca aactccagcc gctgcacatt caccatccca gtcttctcag cagcactgac
     4921 atcaatttgt gtcacccgca gcgcatattt ttcatccagt gcgtttaacc atttcagcag
     4981 gtcattaaac accacaggtt ctatccagac ctgaatattc tccccacgct cggctatccg
     5041 cttgatgacc accgagtgcg cggaagcact gtcactgatg acccgcgata cctgcgcagg
     5101 cgttgttgtg gcagattttc gcgctgcaat aatatccggc gcggcgttct tcagtcgggt
     5161 gttcattgcc accagctgct gcaacatcgt ctcctgttgt tctatccgtt cgctcaacgg
     5221 ctgccagatg aggacgtaat atccgacgct aaacaggaac accaccgctg ccagtaacat
     5281 gcccttttca cgcggagaac gccccgccag atgttgtgtc agccagtgtt cgccacggct
     5341 taactggcgt tcacgccatt gctgaaaata gtgaataaat ttatcgcgta acatgttatt
     5401 tcctccgcaa cgttacgccg ccggaaaccg catcaccctc tttctgtaac gcgtcctgtt
     5461 gcacaacata atctgccgcc agtgcgctac gcagtttgtc gaagctggca aagttcgcag
     5521 cccgtagctg gaggtgaagc gtctggcgtt tttgatcaaa ggtgaaacca cgcatttcga
     5581 tgtcggtaag tgacgctgat ttcagggtac tggcgatcgc tgacaattct gcgagcagcc
     5641 gggtatcgtc ggcctgcggg cgatattttt tcagcgccat cgtcacctga gagcgtaaat
     5701 tcacaatccg cttctgctcc gggaacagtg ttaagaactg tttctccgcc tgggtgcggc
     5761 tttgcgccac ctgttcgctg acgctccata acgtcacgcc ccgttccact gccagcgcaa
     5821 ccagaatcag caatatcggc agaatcatca cccgccagcg cgcccactgt tttcggtagc
     5881 tgacgcgggg ctgccacggc cctgttagca ggttcccttc cggttcgcca taagtggtaa
     5941 tggcgggcag agccgtaacg gtcaggcgtt cagcatctgg gccagcccat gctgatagct
     6001 cttccggtgc aatgccgacc acggttagtg aaagcggtaa atcctgctca ttgagctgtg
     6061 cgcggaacat gaccggagcc agcgccagcc cggcgctcca tccccggcat tcatcgatgc
     6121 ggcagataac ccgttgcgca tcgccagcca taaacccaca aggaatggac atccagtctg
     6181 gcgcgacggt agcgcgggtg atgccgtttg cctgcaacca ctgcgcaatg ttgcgcatat
     6241 gctgttggtg aatcacagct acggttgcca gttgctggtc gattttcaac ggggcgaaat
     6301 gcagttcatc gatatcctgg ttcagctctt cttccagtaa ggcgggcaga atagtcggta
     6361 tctgcttgcg ggcgacatca ggcagttcaa cctgccagac gctgatccat tcgccgggaa
     6421 tgtagagtcg aatcgcatca gtttgcagcc attgctggag ctgttcgttg tcaacgtcag
     6481 accagatgcc gtgctccacg tcggtggtac ggtgctgcca acggatggga tcggaggcgc
     6541 aaagcgggat aaaaatctca agcatggaac tcactcactt tctcctgtct gatgccagag
     6601 aaccgaaaag tgttgtgggc ccatgcggac aattaacgag ttcatcgtca gttcaatctc
     6661 attcacggtg atatctgaac gcagccagaa gtaattgctg tccacgctca ggacggtttt
     6721 tagctgtttt ttagtacgct catcgacgtc agcaagtagc ggctgtgcaa gaaactgatc
     6781 gacatcttcc cagcccttcg ccggacgttg ttgtaataac gcccgcgcct gaacagggct
     6841 taaccacggg tcaaacagcg cctcaagaat cacactttgc gcgacgtcca gggtattaat
     6901 gttgatttgc tggcgggtca tcggcagcgc acagaccagc ggtttcagtt tttgataaag
     6961 tccggcgtcc attccctgca ccacgcgcat ctcgctgata tcagccagcg gttgattagc
     7021 ggcgtaaaac ggcaccgaac gggcgagata ctcgctgtct tcacggccca gacgcgtctg
     7081 cacgctgcgg tcttcgtcaa taaactccca caggctttcg gctatcagtt cggcccgata
     7141 agcaggcaca tccaggcgcg tgatcagggc aatcagttgt tgtaccgcga gcggacgcga
     7201 cgccgtcgtc ggctgagcga gagcattcag gttaaagcag gcctgtgcgt cacgcagagt
     7261 gacggcgatt tgcccctgcg gcagtgggaa aaaacgaggg ccggaagccc acggctgcgc
     7321 cagatgcacg cgcttttcat ttttcaggct cagactgagt gcgctcaacg ccaggctttc
     7381 cgcactggcg ctgtaccaca gcgcctgctg gtactcctgc tggctgcgcg ttcgcccaag
     7441 ttgttgctgc attcgcccgg aaagcgtgat ggtcaccagc atcataaccg ccagcaatac
     7501 cagcaccacg accagtgcca ttccgcgttt tggtggtgag gtgatcatga taattgcggc
     7561 ccgcgtaaca accagatgcg ttcaatctcg ccccattgcg gcgaatgcag agtcatgcgc
     7621 accgccactg gtatcgcctg cactgatgac cagctctcct gccagcgcgt gccgtcgtag
     7681 aactgcaaac ggagcgaatc cgccgggatt aatttttgcg ttgttggctt cacgctgcct
     7741 gccgcatcgg tcagtggcca ggctaaccgt tcgagataac caccatgaat gcggtaaccg
     7801 acggtgagca gattactgcg cggcagacgc atcaacggat taaccacgcc gccgcgcaca
     7861 aaacgcatcc cttcactctc agacgccagc acgccagcgc ccgccagtaa cgctggttca
     7921 cgctggccct gatcgcctct taccggacgc ggcatcattt gtgtcagatc gtggttcaaa
     7981 aaactcatcg tttgctgcat gaggtttagt ttttgatcgt gtccggcgac ggcgctattc
     8041 acgcgtgtaa cgccgtttgt cacctgctgc gccatcagtg ccagtgaggc aaaaatggca
     8101 attgccacca gcatttccag taacgtgaaa ccagcgcaag tccttctcac tgttggcctc
     8161 ccacggcgct aaaccaggcg cgtcgtgact gaatcaccga cgaaaagtct tcgtgacggc
     8221 tgacttcaat atccactgcc tggagcagcg cgttgcctgt actcagcggt gtggttcgcc
     8281 agtaccagcg gcttcctgcc atatcactct cgccctggtg cctgaccgct cgttgttcac
     8341 gggtcatcag gtaaagcagt gcggtctggt tttcagctac ccagcccgct accgtttttt
     8401 cttcaaggat ttgctgatta ctcagcgcgc cgctggcgat ctgtaacacc gccatggcgg
     8461 ccaacgcaaa aatcgccagc gcgagcatta cttccagcaa ggtaaatcca cgcttcatgg
     8521 catcccctca tcacattgat tgagggataa cacgccatcg tgcgccagct ttaccgccca
     8581 acacgcgttt tgtgccgacg cgtaaaaacg cagtgtaaac ggcgtggcgg gttcaaaagg
     8641 cgaaaaacgg atctgcggtg tcttcttttt cgcctctttt tgcagctcca gttcaatatc
     8701 gtgcagcgtc aggcgacgac gctgcagctc cagcgcatat tccttctgcc agatatcact
     8761 tccgggttgt aacagcatct gcacctgttt cggcaccgta acctgtgccg ataaccgggt
     8821 cgcagaaacg ggcaaccact gcccgtgacg acgctgcata aactggtaac ctggtgggtc
     8881 gattagcaca ccgagtgttt tcccttcgat cactgccctg tccttaaact gcgcaaagcg
     8941 agtcagaaaa gcctgcgccg cttttttcgc aggcggctct gaagcggtcg caaacgtctg
     9001 taccacgccc gcactggcaa ggccgataag gaaaatcacc agcatgattt ccagaagtgt
     9061 gaatccgcgt tcaggcactg attactgaaa ctcctgcaaa ttccagttac cgatatctgc
     9121 gccagcgccc tccccgtttt cctgaccatc tgcccccagg gtatacacat caaacagtcc
     9181 tttttcaccc gggctgagat actgataatc attgccccac ggatcttttg gcagtcgttt
     9241 aatgtatcca ccggtacggt agttgcggga gtccgccata ttggccggtt gctggatcag
     9301 cgcctccagt ccctgttcgg tagtcggata acgcccgtta tccagtcggt acatatccag
     9361 cgcattctcc agcgccacga tatcgctgat ggctttttgc cgatcggctt tctctttgtt
     9421 gcccagcagg ttaggcacca ccagacttgc caggacgcca agaataacaa tcaccaccat
     9481 cacttccagc agggtaaaac ctgcccgtgg tttttgtgtg cgggataacg aattcatgtg
     9541 ataactccgt aaattacatt ccaaccatat tgttcagttg cagcatcggt tcgaggatgg
     9601 cgatgacgat aaacagcacc acgcccgcca tcatcaccac cagcgccggc tcaaacagcc
     9661 ctaacgccag ccccacctgg gtatcaaatt ctcgttcctg gttaactgcg gcctgttcaa
     9721 gcatggtttc cagttcaccg ctctgttcgc cggaggcgat catgtacagc atcatcggtg
     9781 ggaacaggcg caactccgcc agcgcagcgc gcagactgct tccttcgcgg acgcgatctg
     9841 ccgccagcag cagttgttgt tcgacatagc gatttgccga cacggcggca gcggtctgaa
     9901 tgccttccag tagcggaaca ctgctggcgg tgaggatgct taacgtgcgg gaaaaacgcg
     9961 ccgtattcag cccgcgcgca acacgccccg tcacgggcaa acgcagcaag gttttatccc
    10021 agcgtaggcg catggcagga tttttgagta gccgttgccc cagcaccaga agccccagca
    10081 atcccgccag ccagtaaacg ccgctggtct gtaacgtgtc gctcatggcg atgagcgtgc
    10141 gggtggaggc aggcagcgcg tgtccgagat gatcaaactg ttcgatgatt ttcggcacca
    10201 ctgccgtcag caaaatagtg actacgcccg ttgccaccac cagcaatacc agcggataga
    10261 gcatggcctg caacagtcgt gatttcaggc gctgtcgctg ttcggtgtaa tccgcaaggc
    10321 gattgagcac cacgtcgaga tgcccggatt tttctccggc agcgaccatc gaacaaaaca
    10381 gggaatcaaa gacgcgggga tgttcgcgca ggctgtccga cagggtataa ccttcctgaa
    10441 tccggctgcg cagcgccatt ccgaggcttt tcacatgcag tttttcactt tgctcactga
    10501 ccgcctgtaa gcaggtttcc agcggcattg ctgcctgcac cagcgttgcc agttggcgcg
    10561 tgaacagcgc caaatctgcc gccgccacgc gacgatgtgc gtgccgccga cgctgcaaca
    10621 ttccccctgt cgaagcattc agccgggctt caatgtgcac ggggataagc tctttaccac
    10681 gcaacaactg gcgggcatga cgcgcggaat ccgcctcaat cattcctttg gttttgcgac
    10741 cattacgctc cagcgcctga tagtaaaaca gtgccattac gcctccatag ttacccgcag
    10801 aacttcatcg agagaggttt ctccggcgag cactttctca atgccgttgc tgcggatgcc
    10861 cgcagagtgt tgccggacat aacgttccag ctccagttcc ccggcctgac ggtggatcaa
    10921 atcacgcaat gtggcgtcca ccacgatcaa ctcatggatg gcagtacgcc cgcggaaacc
    10981 tttgtgatta caggcggggc agccctgcgg atggtacagc gtgacagtac gggcgtcggt
    11041 aatccccagc aggcgttttt cttcgtcagt ggcaggcgcg ggctggcggc agtcggggca
    11101 cagcgtgcgg accagtcgct gcgccatcac gcccgtcagg ctggaagaga gcaggaaagg
    11161 ctcaacgccc atatcctgca gacgtgtgat cgcccccacc gccgtgttgg tatgcagcgt
    11221 ggaaagtacc aggtgccccg tgagcgacgc ctgaacagcg atttctgcgg tttctgtatc
    11281 gcggatttca ccgaccatca ccacatccgg gtcctgacgc aaaatcgcgc gcaggccacg
    11341 ggcaaaggtc atgccgacgc gggtgttgac ctgcgtctga ccgatccctt caatcatgta
    11401 ttcgatgggg tcttcaaccg tgagaatgtt gcgcgaatgg ttgttcagct cctgcaatcc
    11461 ggcgtacagc gtggtgcttt tgccggaacc cgtcggtccc gtcaccagaa agatgccgtg
    11521 cggtttgtgt aacagctggc gcaactgcac ggtcagttgc tgactcaacc ccagacgttc
    11581 cagcgtcagg cgggcctggt ttttgtccag cagtcgcagc accacgcgct cgccccaggc
    11641 ggagggcatg gtggagacac gcacgtcaat cgcccgaccg cccagcagca gcgcaatacg
    11701 cccatcctgc ggtacgcgct tttcggcaat atccagccgc gccatcacct tgatacgcga
    11761 taccagcagc gaggccagtt tgcgccccgg gcgcagcatt tcatgtaatg tgccgtcaac
    11821 acgaaaacgg atcaccagac tcttttcaaa tgtctcgatg tggatatccg aagcgccttc
    11881 tttaatcgcc tctgccagca tggcgttgat cagtttgatg attggcgcgt cgtcgtcgct
    11941 ttccagcaga tcttccgttt ccggcagttc ttcggcgagg gtaaaaaaat cctcagcaga
    12001 accgagatct tccatcagct ggcgggcctc ggaagagtcc cgctgccaga ccgcattcag
    12061 ccgctgttca aattcggctt cgtcgatttg ccgcagcgta aagggcgcgt tcagcccccg
    12121 ctgcagctcc tgcaaaacag agagcgacaa cggatgaacg tggaggatct ccagcgacgc
    12181 ttcgcaccat gccaccaggc taaaccgacg gctgaaactg tagggcagac gcacggtgtt
    12241 agcggtggtt tcctgtgcta caggcaccat taacgcgttc tcccggcatt gaggaatgcg
    12301 cgaacttccg gcggtaaggc ctggttttgc gctggcagta ccggctgcgc ggtgtgcggc
    12361 atcaggctta agccttgatc atcgcgatag atctgctcgg cgcgcatgta gttatatttg
    12421 cgctgcgaca cgccgtctgc cgccataccg tcacgcagaa tggtcgggcg gataaacacc
    12481 atcaggttac gtttttcttt tttatccgcc gtcgatttaa acaggttacc aatcaacggg
    12541 atatcgccca gcagcggcac tttcgccacg ctttctcccg cctggtcgtc catcagaccg
    12601 ccaagcacga tcagttcacc atcgttagcc agcacggtgg tcttcagttt gcgctcacca
    12661 aacaccacgt cgaggctggt ctgtccttcc accttcgaca cttcctgctc aatcaccatc
    12721 tgtaccgcgt ttccttcgtt aatctgcggc gtgactttca gcatgatgcc gacttttttc
    12781 ctctctaccg tattgaaagg attgctgtta ttggagccaa cggtagatcc agttaatacc
    12841 ggaacgtcct ggcccaccat gaagaaggct tcctggttgt ccagcgtggt gatgctcggc
    12901 gtggagagca cgttcgagct ggagtcgttt ttgaccgcct gtaccagtgc catccagtcg
    12961 cctttcacca cgccaaccgc cgtaccgcta aagccagaaa gcagctgggc gagcgtggag
    13021 agatcgccgt tggtatccgg gttaatggtg gtggcaccgt tttcactgat caccgtggag
    13081 cctttctgcg gtttcgcctg agaaatggct gcccccagcg tgccgatagg gatctgcgta
    13141 ccgttagcaa actgcattaa tccggcatct ttcgacgccc actgcacgcc gaaattgata
    13201 ttgctgcctt cagcaacttc cacgatcaac gcctcgacgt gcacctgagc acggcgaata
    13261 tccagttgtt caatcacgct ttgcagcgac tgcatgatgt cctgcggcgc agtaacaatc
    13321 agggcattac tgtgtttgct ggcggcgatg gagacaacct cacgcccgct accgacggtg
    13381 ccttccgcct cttctttagc cgccgtgagc gtgccgctga cctgcttcag cacatcgacc
    13441 agatcttccg ctttgctgta tttgagatag aaaacctggc tgttgccgct gcgctccatt
    13501 tctgagtcca gccgacggat caggcggcgc attttgtccc gcgtggccgg gtcaccactg
    13561 acaatcacac tgttggtgcg ttcgtcggcg acaatttgag atttcagcgt cgcaggttgg
    13621 ttctcgccgc tgtttttcgt caggctttcc agcacgcggg cgatttccga agccgaggcg
    13681 ttatccagcg ggatcacctc ttcagtgcga ttacccgcgt gatccacacg ctggatcact
    13741 tccgtcagcc gctccacgac ggaggcgcgc ccggtgagca taatcacgtt ggagggatcg
    13801 taattaacaa cgttgcctga gcctgcgctg tcaatcatct ggcgcagaat cggtgccagc
    13861 tcgcgtaccg acacattgcg taccggcacg actttggtaa ccatttcatc gcccgcgtag
    13921 ttgtcgctgc cttcaccgac cagcggcagc ggctcgactt tcgcggcgct tgatttcacc
    13981 accttcagca cgtcgttttc catcggtacg accgcatacc cctgtgcttc cagcaggtta
    14041 aggaataact ggtaatactg gcgttcattg agcggagtca tagtgcgaat actcactttc
    14101 ccctgaacgc ccggccccat aatgatggtt ttattaaggt tagcgccgac ggtttcgatg
    14161 aacgatttca ggtcagtatc tttaaaatta gcggtgaaag tggcttcttc cgcccagacc
    14221 ggtgaactac acaatgccgc tgccagcatc agcggcagta aacgcttttt tgttttgagg
    14281 ccagttgtct tcttacgcca gatagacaac gtcatatcac gccaaaacac gatgaatgat
    14341 tctcctggat taaatgcggt tagcgcagtg cgatggaaat gtcgtagcgc gcacccttgc
    14401 gtaaaaccgt aagttgaatg gaatccattg aaggtaactg ccgcatcaga gcaatcattg
    14461 ctcgtggatc agtgaaatcc tgctgattta gtgcaatggc gatatcgcct tccctgaaac
    14521 cgctggcatc gaacagagaa cgatctgccc ccggcttcac tgcataaccg acaattccct
    14581 ccttacgcac aggcgtaagc tggatatagt taaaaatttt ctgcggatct ttcgccagtg
    14641 cctgacgcac ggcagccggg atctcaactg gcgcactggc agcaggctca gcaacagctt
    14701 gctttgcttc gtcgctgacg gcttttttgc tggtgaccgc cacagggggg cgttcctcct
    14761 ctgccagact cagacgttcc attttcccct gatagcgcag catcacatgg tcgcggttga
    14821 tttcctcaat cactgcgttg tgagagccaa gccgttcacc ctgcaaatag acctgctgtt
    14881 tgccgccttc ttcaatcacc gcgccgggtc tggcaccaaa ggcgatccca cgcagcacca
    14941 cattaagacg cgtttctgcc acaggcgcag attcaggttg ttttaccggt gcggcgacgg
    15001 gttgatattt gccaaaccag ttttgttggc tgactaattg cacgtcattt ttatcgaacg
    15061 gttttgcatc cgcacggagc ggtttattcg ccgatgagga aaccaccgta tattccgcag
    15121 aaaaggagaa atagcgccag agtgaatacg ccatttttgc agaaataata agcagcatga
    15181 gccagaacat cccgcgcacc atttttcgca gatgctcttt gtctgtatta agagattgtc
    15241 tctggctaag aatgtgacga attttagtca gccattgaat gagataaatt cgtgcgtcac
    15301 gaaaaacaac ccgcgccaat ttatatctac ccgacgttat gctttgacta ttccacaggt
    15361 ggtacgatcc agtttccgct gatactggtt acctgtacgt gtatcattaa tcgttatgct
    15421 gtaattaatt ccctcggtga tcattaattt caccgttggg tcagcgcaca tttgtcgttg
    15481 atagctggtt aaaaaggcgt caggtgtctg cgtggtctga gtacccgatt cgctgataat
    15541 ggtcattttt accgtcgtgc cactactttg cgccagcact aaggtatatc ccgcagattt
    15601 aatcggcagg ttttggctga tattttgcgc ctgttttttc gccagtaaac tggcgttttc
    15661 attatggctg gcgcagccac ttaataatcc tgtaacgctc aacaatagcg atattaatac
    15721 cctccctggc atttgtttta tcgacatggt aaataatctc taaggttatt aataagagtt
    15781 aaaatgtcac tttgataatg acgttgttat cattaaaaca atgcctttag ataaagtgtt
    15841 gctataccgc ccagacttaa acacggtcca aaaggcagtg tggttgatcc tctttttgta
    15901 ataacggcat atatcaggcc gcagcatgag gcgattaaag caacgttggg cagtgataac
    15961 gcccccaccc agccacctaa agcggcgaag agtaatacat cgcccatgcc taatgcttct
    16021 ttacgcagaa ctattccggc tatccagcgc agggagtaaa aagtgataaa tcccaccagt
    16081 acgccggtga ctgcgtcttg tagcgtgagc ggactctgct gcgcccatgc cgcaattagt
    16141 cccgtccaca atacgccctg agtaaaaaca tcgggcagcc attggttatc gaggtcaatg
    16201 acgctcgcgg caatcagcca ggcggataat atcatcaccg ccagcgccca tccactttcc
    16261 ggccacacca ggctcgccag caaaaaagcg agtgccgtca ataactccac cagcggataa
    16321 cgcttgctga ttttcgcctg acagtcgcgg cagcgccctt tgagcatcaa ccaggagagc
    16381 agcggaatat tgtcacgaac gcggatagtc tgctggcaat gcggacagtg cgagcgtggc
    16441 agcgccaggc ttatttttga ctgcgtactc ggcatttcac cgtgaaactc cgccatttgt
    16501 tggcgcagca tgatggggta acgccaaatc accacattca aaaaactgcc gataatcaat
    16561 cctccgacgg tagccaggac gggcatcgcc gcggggtatt gctgaaaaac atcaaaaaac
    16621 atggttaaag gttatttgtt gtaacttgcc ggatgcgggc ctgcaggtgt atgccatacg
    16681 gctttcctcc aggcccgatg cgccttattt catgccggat gcggcgcgag cgccttatcc
    16741 ggtatacagg cttactcagc tgacatctta tgctcggtaa cctgattaat ggtttccggc
    16801 ccttgctgcg gtttcggcag attaagcgcc gccagcgtct cgtaagccga ctggctcaca
    16861 ccgccctcga agttcatctc gctcgctccc ggcaactggt aagcattcgc gcccggattc
    16921 catttcttaa agaactccga aagatccgtc tgggcgaccc aggaggcaca cagcatcagc
    16981 gtgtccgctg cgttaccgtt ggattcagca cagtagttct tactgccgaa cttgttattg
    17041 ccaacctcat cgccgcgcgc tttacggtgc atcaactgga acaggttcca gcctttcatc
    17101 ccttcacgct cgctgtaaaa cttaggcagg tcaccttctg ggtaccactg tttgatatca
    17161 aagtttttct ctgcccactc tttcagctgt gcgtacatca gcagacggtc ccccgcaccg
    17221 ccgcgcgccc aggcctgacc gttgctctcg tccagatatt ccggcgcgac ggtaatgtcg
    17281 tcagcgacac ggttcatctt gccgagatag cgatcctgca tgtacagcgc cagcacgttg
    17341 ttcgccactt cagttgcacc cggtacgttc agcggtgttt ctgcagcgtt atgaccggct
    17401 tcgtgccaaa tcagccagtc gttcagcggc gttgtcggca gcgtggtgct gttcgtcgag
    17461 aagctgctgt tcattaccgg ataacccgag tgcgcatcac cgatggagat ctgcacatcg
    17521 ttggcgaaac ggtgtttatg ccccgtcaat gcttcatagg taaacatccg gtggttaccg
    17581 ctcgtttcat tgcgaccgta gaagtcattc atcgagctgg caaaggtatc cagatcttta
    17641 gcgaattctg ctacaccacc agtgaaattg ctggcctcaa ggttcttctt cggcgtggta
    17701 tagacgaacg acgcagactc cagctcgccc agcggcgccg gtgagtccag atcgtttttc
    17761 cattcgccgt ctttatagaa cggcgctttt actacaccgg taaaggtgaa gttagcagaa
    17821 acatcatcct tactgtcgcc cttgatataa atcagaccac cataaggcac cttgaaggtc
    17881 acttcaccgt tagcctccag agtatacgtt ttagtcactc ttggcggacg gttcagcgca
    17941 acttcatgct tctcacgtcc agtcaggtcg tcagccagcg ccacggtaac agtcactggg
    18001 actgacgccg aagacttaat ggtgacgtcc tgctgggccg gtgcccacag gccggttgac
    18061 tgcatgttac ccgcaaacca tttggtcgga ttcgagtaca ggctgatgtt ttccgtaacg
    18121 ctctcaccct ttgccgatac ggatcctggg tacttctcca catcaacctt aatgttcaga
    18181 tcccaccagg aacggcccag catcagacgc gtcagcggtt tttccatata gttgagcgga
    18241 tagcttgggt tcatcatgcc cgctttgctg ctaccgtcac cgtagatcat gttgttatcg
    18301 accagcgatt ttttcagatc tgcggagcac ttggtgccgc ctgcataggc atcattggcg
    18361 tagcagttca ggaactcggt gaacgtttta aagcccagct catcttcctt gccctcttcg
    18421 taacgatatt tcgtatcgtt ccacagccag accgacatgt tctggtacag acgttccaga
    18481 tcaacactgt tcagacgctc acctttactg cctttggtac ggaaataaag ctcatgctga
    18541 tacaggcgct gaatgttggt gcctaaatcc gccgcctgca ccatcgcttt cgcggtgtcg
    18601 gcgtcaagat tcagttgcgt atagcgcgga acatacatgc cacctgttac cggaacatcc
    18661 gtgcctgggc ggcgctccaa acagttaatc tcgtaatggt aagtcgagtc cttacactcc
    18721 tgtaacccag gaaacttctc aaagattttt gcctttgccg cttccagaga ttcttctgtt
    18781 gtgtattccg cttcatcaat aaaggcataa cgcgttacct gtttgccctc aacttcctcc
    18841 tgccagctcg caacttccag tttcggcttg tcatcaggct tgttgtcttg ctggtatttc
    18901 caggtcactt cccctgtatt tgggtcgatg gtgtacggcg gttgcgcgcc gtctgcagca
    18961 ggataacgtt cataaaccca aatgccagtc gcgcgacgct gacgaacgcg atccggatac
    19021 ccttgcggat cgttgttcac caccgatttg ttcagagcca ttgacagacc cgcggcatcc
    19081 agcagacgca caaaactgga cgcgctctct tccttaagat tgctcatcac gttttccatg
    19141 atcagcaccg agccaccttt gttcagataa gcgatcagat cggtcacatc ctgctgagtc
    19201 agcttcggtt tgctggtatc tgcacgcaga ggcacagcat agggatcgcc agaccactga
    19261 gtcacatatt caaagccgtt gaggatcagc aacggaatct cttccggatt cagatcgcca
    19321 tagctggtca actgtttaac cgtgatacca gtgaaatcct catggaaagc aaatggtgca
    19381 ctatttccca atacctggcc cgcttttttg aaataaacgt tctccaggtt ggtgccgaca
    19441 gtcatgatgc tcttggtatt tggctgccag atgtcatttg acaagtagcg caggacgttc
    19501 tgcataaagt gcttcatgtc atcagaatca ccgctgagcg tacactcacc tttactatta
    19561 acaccaccgc cccaactgta accgttcggg caacgcagga tgctgttgta gtgcgggtta
    19621 ccgataacca tcagtttgcc ttcaccgact tgccccagcg aaataaacgg caggttgaaa
    19681 gtcgcagtat cgcgcgtaac gttctctggc tgcacaatgg aaggcgcttc cgtaatgtac
    19741 gccagctcat ttttatccca ggcgcgtttt tcgccaaacg ccagccagta gtttttatca
    19801 ttacgcgcca tcagaatcgg gaatgccgag ttggagatat ttaccaccgc ctgaccgcgc
    19861 gcgttaccgg tgctgccata gaagttggta gagtcatgga agacgtggaa cttgctgaca
    19921 gactgatagt tcgtatccac gccccacagc ttgttaataa cgccctgaat ctggccgtca
    19981 ttaacattgc gcgttgtcag cgagaaccag cgagcctcgt tacaaccgtc ggttttcgca
    20041 caaatcgcgg tatcgatctc tttggcctga cccgtcttaa actgctcgat aaattcgtta
    20101 ggcagcacaa cgttttgatc gccttcatcc agcgtcgcac cgttggataa cgaaagattg
    20161 attatctcgt tgatcacgtt gggatattcg gcaaagacct tgcgtacata gtccggaaca
    20221 acacgagtat tattttgacc agtcgtcgaa taacgatgaa tgagctgatc gatatttgcc
    20281 ccgcgaactt catcacccaa ttcagtcagc gcaatggtcg acttattgcc acgtactgag
    20341 cccagttcaa aggtatcgat accaaaggag atgtttcgcc ccagctaaag gagaatttac
    20401 cgttttcgtc agttacgcca cggcctgaat tggtgtagta gtcaacgcca gcaacaccgt
    20461 tccccaggct atccaccagt tggccttcgg aaagaatgat ttcagtgggt tgatactgat
    20521 aaaactgttc cgcgttagcc gacacgaagg acgcgttcag atccggtttt gttccctctg
    20581 tcgtgactgg cactaccgtt gaggtatggg tggacggcgc tttatcagtc gcagcattgt
    20641 tttccacctc ttcattgacc agcttgctga aattgtctgt tgccagatca atttgcttat
    20701 acagtttttc aaatcgcgcg cgatcaacca ctgacgagaa agtaagacaa agctgttctg
    20761 catctgcggg gcaactgtcg ctggacgtca ccagagagat ggcgttggtt ttcttatttt
    20821 cagaattcgc cagctcctgc gcgtcctcca ggctaaacga cactttgtca accgcacgca
    20881 ggctacgcgc agcttctgac tgggtgttga atgttgcaat ggtcgtactg cccaccacac
    20941 aactcacggt attgcctggc gtaaaggtaa agccatcgct ggattcacca ttacaggtag
    21001 caccagttac ccgctggctt ccgcccaggg tcagataacc cgttttcgta ggaacaggtt
    21061 ctggctccgg ttctggctct ggatcaggtg tcggttccgg cgtaggttct gggtccggcg
    21121 ttggctcagg cgtcggctcc gggtttggtg ttggatcagg tttcacttcc ggcaaagacc
    21181 ctgttccaga atctacaggc ggcgtatcgg aggaagatcc ggaaccaccg ccatc
//
LOCUS       DAZFLD010000073        21014 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.073, whole genome shotgun sequence.
ACCESSION   DAZFLD010000073 DAZFLD010000000
VERSION     DAZFLD010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21014
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.073"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(2..334)
                     /gene="emrE"
                     /locus_tag="ACHKBL_002294"
     CDS             complement(2..334)
                     /gene="emrE"
                     /locus_tag="ACHKBL_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter EmrE"
                     /protein_id="HFO5819159.1"
                     /translation="MNPYIYLGGAILAEVIGTTLMKFSEGFTRLWPSVGTIICYCASF
                     WLLAQTLAYIPTGIAYAIWSGVGIVLISLLSWGFFGQRLDLPAIIGMMLICAGVLVIN
                     LLSRSTPH"
     gene            complement(402..704)
                     /locus_tag="ACHKBL_002295"
     CDS             complement(402..704)
                     /locus_tag="ACHKBL_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ren"
                     /protein_id="HFO5819160.1"
                     /translation="MTGKEAIIHYLGTHKSFCAQDVAAVTGATVTSINQAAAKMARAG
                     ILVVDGKVWRTVYYRFATREEREGKVSTNLIFKECRQSAAMKRVLRVYKRTSMGTQ"
     gene            complement(701..1402)
                     /locus_tag="ACHKBL_002296"
     CDS             complement(701..1402)
                     /locus_tag="ACHKBL_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein P"
                     /protein_id="HFO5819161.1"
                     /translation="MKNIAAQMINFDREQMRRIANNMPEQYDEKPQVQQVAQIINGVF
                     SQLLATFPASLANRDQNELNEIRRQWVLAFRENGITTMEQVSAGMRVARRQNRPFLPS
                     PGQFVAWCREEASVTAGLPNVSELVDMVYEYCRKRGLYPDAESYPWKSNAHYWLVTNL
                     YQNMRANALTDAELRRKAADELSCMTARINRGEAIPEPVKQLPVMGGRPLNRAQALAK
                     IAEIKAKFGLKGASV"
     gene            complement(1399..2418)
                     /locus_tag="ACHKBL_002297"
     CDS             complement(1399..2418)
                     /locus_tag="ACHKBL_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFO5819162.1"
                     /translation="MTGSEMAKAGLLEQNRLSGANRNTLIAGGIMANTAEIFNFPVPD
                     AAQKEPRVADLDDGYTRIANELLEAVMLAGLTQHQLLVFLAVMRKTYGFNKKLDWVSN
                     EQLSELTGILPHKCSAAKSVLVKRGIFIQSGRNTGINNVVSEWSTLPESGKKNKVYLK
                     EVNLPESGKKSLPKSGKGVYPNQVNTKDKLTKDNIKPFSSENSGESSDQPENDLPVVK
                     PDAAIQSGSKWGTAEDLTAAEWMFDMVKTIAPSARKPNFAGWANDIRLMRERDGRNHR
                     DMCVLFRWACQDNFWSGNVLSPAKLRDKWTQLEINRNKQQAGVTASKPKLDLTNTDWI
                     YGVDL"
     gene            complement(2415..2954)
                     /locus_tag="ACHKBL_002298"
     CDS             complement(2415..2954)
                     /locus_tag="ACHKBL_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001182892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT domain-containing protein"
                     /protein_id="HFO5819163.1"
                     /translation="MQPLTYQQTSGFSPTAVINRSQTKQVPGHEKIRDAVRAWSAVDN
                     QDVVATLIVNEYREQGGGTIDFPDDVSRARQKLFRFLDNKFDSEKYRNNVRELTPAIL
                     AVLPLEYRGYLVEQDSFMARLAEMEKELSEAKQAVILNAPRHQKLKEISEGIVSMFRV
                     DPDLAGPLMAMVTTMLGAI"
     gene            complement(3024..3254)
                     /locus_tag="ACHKBL_002299"
     CDS             complement(3024..3254)
                     /locus_tag="ACHKBL_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO5819164.1"
                     /translation="MNPAIKTAINIVGSQKKLGAACEVSQQAVYKWLHNKAKVSPEHV
                     GSIVTATGGVVKAYQIRPDLPKLFPHTEKNAA"
     gene            3359..4048
                     /locus_tag="ACHKBL_002300"
     CDS             3359..4048
                     /locus_tag="ACHKBL_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000858971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HFO5819165.1"
                     /translation="MKTTLSERLKEARLARGLTQKALGDLVGVSQAAIQKIETGKANQ
                     TTKIVEIANALGVRAEWLSSGVGNMSDSTVQPIQSTVSHSKYFKIDVLDIEVSAGPGV
                     INREFVEVLRSVEYSFDDARHMFDGRKAENIRIINVRGDSMSGTIEPGDLLFVDITVK
                     SFDGDGIYAFLYDDTAHVKRLQMMKDKLLVISDNKSYSPWDPIEKDEMNRVFIFGKVI
                     GSMPQTYRKHG"
     gene            4644..4850
                     /locus_tag="ACHKBL_002301"
     CDS             4644..4850
                     /locus_tag="ACHKBL_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001524508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibitor protein"
                     /protein_id="HFO5819166.1"
                     /translation="MVHQHYGTQTVNRGAVMPGMLVKHKDGTWTASANLRGRLYLHRG
                     IERTYTRDLLVEVFLDGRGNGLNH"
     gene            4926..5222
                     /locus_tag="ACHKBL_002302"
     CDS             4926..5222
                     /locus_tag="ACHKBL_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0060703 - deoxyribonuclease inhibitor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor Gam family protein"
                     /protein_id="HFO5819167.1"
                     /translation="MNAYYIQDRLEAQSWARHYQQIAREEKEAELADDMEKGLPQHLF
                     ESLCIDHLQRHGASKKAITRAFDDDVEFQERMAEHIQYMVETIAHHQVDIDSEV"
     gene            5228..6013
                     /gene="bet"
                     /locus_tag="ACHKBL_002303"
     CDS             5228..6013
                     /gene="bet"
                     /locus_tag="ACHKBL_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage recombination protein Bet"
                     /protein_id="HFO5819168.1"
                     /translation="MSTALATLAGKLAERVGMDSVDPQELITTLRQTAFKGDASDAQF
                     IALLIVANQYGLNPWTKEIYAFPDKQNGIVPVVGVDGWSRIINENQQFDGMDFEQDNE
                     SCTCRIYRKDRNHPICVTEWMDECRREPFKTREGREITGPWQSHPKRMLRHKAMIQCA
                     RLAFGFAGIYDKDEAERIVENTAYTAERQPERDITPVNDETMQEINTLLIALDKTWDD
                     DLLPLCSQIFRRDIHASSELTQAEAVKALGFLKQKATEQKVAA"
     gene            6010..6690
                     /locus_tag="ACHKBL_002304"
     CDS             6010..6690
                     /locus_tag="ACHKBL_002304"
                     /EC_number="3.1.11.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lambda exonuclease family protein"
                     /protein_id="HFO5819169.1"
                     /translation="MIPDIILQRTGIDVRAVEQGDDAWHKLRLGVITASEVHNVIAKP
                     RSGKKWPDMKMSYFHTLLAEVCTGVAPEVNAKALAWGKQYENDARTLFEFTSGVNITE
                     SPIIYRDESMRTACSPDGLCSDGNGLELKCPFTSRDFMKFRLGGFEAIKSAYMAQVQY
                     SMWVTRKDAWYFANYDPRMKREGLHYVVIERNEKYMASFDEMVPEFIEKMDEALAEIG
                     FVFGEQWR"
     gene            6687..6869
                     /locus_tag="ACHKBL_002305"
     CDS             6687..6869
                     /locus_tag="ACHKBL_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1317 domain-containing protein"
                     /protein_id="HFO5819170.1"
                     /translation="MTHPHDNIRVGAITFVYSVTKRGWVFPGLSVIRNPLKAQRLAEK
                     INNKREAVCTKHLLLS"
     gene            6842..7033
                     /locus_tag="ACHKBL_002306"
     CDS             6842..7033
                     /locus_tag="ACHKBL_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1382 family protein"
                     /protein_id="HFO5819171.1"
                     /translation="MHKASPVELRTSIEVAHSLAQIGVRFVPIPVETDEEFHTLATSL
                     SQKLEMMVAKAEADERDQV"
     gene            7110..7325
                     /locus_tag="ACHKBL_002307"
     CDS             7110..7325
                     /locus_tag="ACHKBL_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFO5819172.1"
                     /translation="MTPQQENALRSIARQANSEIKKARQHFPDKNVDDICRSVLKKHR
                     ETVTLMGFTPTHLSLAIGMLNGVFKER"
     gene            7424..7633
                     /locus_tag="ACHKBL_002308"
     CDS             7424..7633
                     /locus_tag="ACHKBL_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000763384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HFO5819173.1"
                     /translation="MADIIDSASEIEELQRNTAIKMRRLNYQTVSATHCCECGDPIDE
                     RRRLAVQGCRTCASCQEEIELKNKQ"
     gene            7690..7968
                     /locus_tag="ACHKBL_002309"
     CDS             7690..7968
                     /locus_tag="ACHKBL_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016248605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFO5819174.1"
                     /translation="MFRIIFPNTWYVDHHGTPCKILRSTHNKVHYIRKGRTCIASMFR
                     FNHDFEPVNKADADRIAEEIETAEHIKKLRDMRSKSRGNHGIIQPHTR"
     gene            7940..8005
                     /locus_tag="ACHKBL_002310"
                     /pseudo
     CDS             7940..8005
                     /locus_tag="ACHKBL_002310"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            8167..9330
                     /gene="intD"
                     /locus_tag="ACHKBL_002311"
     CDS             8167..9330
                     /gene="intD"
                     /locus_tag="ACHKBL_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HFO5819175.1"
                     /translation="MSLFRRNEIWYASYSLPGEKRIKESLGTKDKRQAQELHDKRKAE
                     LWRVEKLGDLPDVTFEEACLRWLEEKADKKSLDSDKSRIEFWLEHFEGIRLKDISEAK
                     IYSAVSRMHNRKTKEIWKQKVQAAIRKGKEPPVYEPKPVSTQTKAKHLAMIKAILRAA
                     ERDWKWLEKAPVIKIPAVRNKRVRWLEKEEAKRLIDECPEPLKSVVKFALATGLRKSN
                     IINLEWQQIDMQRRVAWVNPEESKSNRAIGVALNDTACKVLRDQIGKHHKWVFVHTKA
                     AKRADGTSTPAVRKMRIDSKTSWLSACRRAGIEDFRFHDLRHTWASWLIQSGVPLSVL
                     QEMGGWESIEMVRRYAHLAPNHLTEHARKIDDIFGDNVPNMSHSGIMEDIKKA"
     gene            complement(9346..9422)
                     /locus_tag="ACHKBL_002312"
     tRNA            complement(9346..9422)
                     /locus_tag="ACHKBL_002312"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9386..9388),aa:Arg,seq:tct)
     gene            9693..10559
                     /gene="folD"
                     /locus_tag="ACHKBL_002313"
     CDS             9693..10559
                     /gene="folD"
                     /locus_tag="ACHKBL_002313"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HFO5819176.1"
                     /translation="MAAKIIDGKTIAQQVRSEVAQKVQARIAAGLRAPGLAVVLVGSN
                     PASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDALNADNTIDGILVQLPLP
                     AGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPRLRPCTPRGIVTLLERYNIDTFGL
                     NAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKPGFIPG
                     DWIKEGAIVIDVGINRLENGKVVGDVVFEDAAKRASYITPVPGGVGPMTVATLIENTL
                     QACVEYHDPQGE"
     gene            10561..10773
                     /gene="ybcJ"
                     /locus_tag="ACHKBL_002314"
     CDS             10561..10773
                     /gene="ybcJ"
                     /locus_tag="ACHKBL_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein YbcJ"
                     /protein_id="HFO5819177.1"
                     /translation="MATFSLGKHPHVELCDLLKLEGWSESGAQAKIAIAEGQVKVDGA
                     VETRKRCKIVAGQTVSFAGHSVQVVA"
     gene            10881..11402
                     /gene="ybcI"
                     /locus_tag="ACHKBL_002315"
     CDS             10881..11402
                     /gene="ybcI"
                     /locus_tag="ACHKBL_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFO5819178.1"
                     /translation="MPTVITHAAVPLCIGLGLGSKVIPPRLLFAGIVLAMLPDADVLS
                     FKFGVAYGNVFGHRGFTHSLVFAFVVPLLCVLIGRRWFRAGLIRCWLFLTVSLLSHSL
                     LDSVTTGGKGVGWLWPWSDERFFAPWQVIKVAPFALSRYTTPYGHKVIISELMWVWLP
                     GMLLMGMLWWRRR"
     gene            complement(11438..12823)
                     /gene="cysS"
                     /locus_tag="ACHKBL_002316"
     CDS             complement(11438..12823)
                     /gene="cysS"
                     /locus_tag="ACHKBL_002316"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HFO5819179.1"
                     /translation="MLKIFNTLTRQKEEFKPIHAGEVGMYVCGITVYDLCHIGHGRTF
                     VAFDVVARYLRFLGYKLKYVRNITDIDDKIIKRANENGESFVALVDRMIAEMHKDFDA
                     LNILRPDMEPRATHHIAEIIELTEQLIAKGHAYVADNGDVMFDVPTDPTYGVLSRQDL
                     DQLQAGARVDVVDDKRNPMDFVLWKMSKEGEPSWPSPWGAGRPGWHIECSAMNCKQLG
                     NHFDIHGGGSDLMFPHHENEIAQSTCAHDGQYVNYWMHSGMVMVDREKMSKSLGNFFT
                     VRDVLKYYDAETVRYFLMSGHYRSQLNYSEENLKQARAALERLYTALRGTDKTVAPAG
                     GEAFEARFIEAMNDDFNTPEAYSVLFDMAREVNRLKAEDMAAANAMASHLRKLSAVLG
                     LLEQEPEAFLQSGAQADDSEVAEIEALIQQRLDARKAKDWAAADAARDRLNEMGIVLE
                     DGPQGTTWRRK"
     gene            12997..13491
                     /gene="ppiB"
                     /locus_tag="ACHKBL_002317"
     CDS             12997..13491
                     /gene="ppiB"
                     /locus_tag="ACHKBL_002317"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase B"
                     /protein_id="HFO5819180.1"
                     /translation="MVTFHTNHGDIVIKTFDDKAPETVKNFLDYCREGFYNNTIFHRV
                     INGFMIQGGGFEPGMKQKTTKDPIKNEANNGLKNTRGTLAMARTQAPHSATAQFFINV
                     VDNDFLNFSGESLQGWGYCVFAEVVEGMDVVDKIKGVATGRSGMHQDVPKEDVIIESV
                     TVSE"
     gene            13494..14216
                     /gene="lpxH"
                     /locus_tag="ACHKBL_002318"
     CDS             13494..14216
                     /gene="lpxH"
                     /locus_tag="ACHKBL_002318"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HFO5819181.1"
                     /translation="MATLFIADLHLCAEEPAITAGFLRFLAGEARKADALYILGDLFE
                     AWIGDDDPNPLHRQMAAAIKAVSDSGVPCYFIHGNRDFLLGKRFARESGMTLLPEEKV
                     LELYGRRVLIMHGDTLCTDDAGYQAFRAKVHKPWLQTLFLALPLFVRKRIAARMRANS
                     KEANSSKSLAIMDVNQNAVVSAMEKHQVQWLIHGHTHRPAVHELIANQQPAFRVVLGA
                     WHTEGSMVKVTADDVELIHFPF"
     gene            14334..14843
                     /gene="purE"
                     /locus_tag="ACHKBL_002319"
     CDS             14334..14843
                     /gene="purE"
                     /locus_tag="ACHKBL_002319"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HFO5819182.1"
                     /translation="MSSRNNPARVAIVMGSKSDWATMQFAAEIFEILNVPHHVEVVSA
                     HRTPDKLFSFAESAEENGYQVIIAGAGGAAHLPGMIAAKTLVPVLGVPVQSAALSGVD
                     SLYSIVQMPRGIPVGTLAIGKAGAANAALLAAQILATHDKELHQRLNDWRKAQTDEVL
                     ENPDPRGAA"
     gene            14840..15907
                     /gene="purK"
                     /locus_tag="ACHKBL_002320"
     CDS             14840..15907
                     /gene="purK"
                     /locus_tag="ACHKBL_002320"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HFO5819183.1"
                     /translation="MKQVCVLGNGQLGRMLRQAGEPLGIAVWPVGLDAEPAAVPVQQS
                     VITAEIERWPETALTRELARHPAFVNRDVFPIIADRLTQKQLFDKLHLPTAPWQLLAD
                     RSEWPAVFDRLGELAIVKRRTGGYDGRGQWRLRANETEQLPAECYGECIVEQGINFSG
                     EVSLVGARGFDGSTVFYPLTHNLHQDGILRTSVAFPQANAQQQAQAEEMLSAIMQELG
                     YVGVMAMECFVTPQGLLINELAPRVHNSGHWTQNGASISQFELHLRAITDLPLPQPVV
                     NSPSVMINLIGSDVNYDWLKLPLVHLHWYDKEVRPGRKVGHLNLTDSDTSRLTATLEA
                     LIPLLPPEYASGVMWAQSKFS"
     gene            complement(16240..17133)
                     /gene="ybcF"
                     /locus_tag="ACHKBL_002321"
     CDS             complement(16240..17133)
                     /gene="ybcF"
                     /locus_tag="ACHKBL_002321"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFO5819184.1"
                     /translation="MKTLVVALGGNALLQRGEALTAENQYRNIASAVPALARLARSYR
                     LAIVHGNGPQVGLLALQNLAWKEVEPYPLDVLVAESQGMIGYMLAQSLSAQPQMPPVT
                     TVLTRIEVSPDDPAFLQPEKFIGPVYQPEEQEALEAAYGWQMKRDGKYLRRVVASPQP
                     RKILDSEAIELLLKEGHVVICSGGGGVPVTKDGAGSEAVIDKDLAAALLAEQINADGL
                     VILTDADAVYENWGTPQQRAIRHATPDELAPFAKADGSMGPKVTAVSGYVRSRGKPAW
                     IGALSRIEETLAGEAGTCISL"
     gene            complement(17130..17945)
                     /gene="ylbF"
                     /locus_tag="ACHKBL_002322"
     CDS             complement(17130..17945)
                     /gene="ylbF"
                     /locus_tag="ACHKBL_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HFO5819185.1"
                     /translation="MTIIHPLLASSSAPNYRQSWRLAGVWRRAINLMTESGELLTLHR
                     QGSGFGPGGWVLRRAQFDALCGGLCGNERPQVMAQGIRLGRFTVKQPQRYCLLRITPP
                     AHPQPLAAAWMQRAEETGLFGPLALAAGDPLPAELRQFRHCFQAALNGVKTDWRHWLG
                     KGPGLTPSHDDTLSGMLLAAWYYGALDARSGRPFFACSDNLQLVTTAVSVSYLRYAAQ
                     GYFASPLLHFIHALSCPKRTAVAIDSLLALGHTSGADTLLGFWLGQQLLQGKS"
     gene            complement(17956..19215)
                     /gene="ylbE"
                     /locus_tag="ACHKBL_002323"
     CDS             complement(17956..19215)
                     /gene="ylbE"
                     /locus_tag="ACHKBL_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009029994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HFO5819186.1"
                     /translation="MFTSVAQANAAVIEQIRRARPHWLDVQPASSLISELNEGKTLLH
                     AGPPMRWQEMTGPMKGACVGACLFEGWAKDEAQALAMLDQGEVNFIPCHHVNAVGPMG
                     GITSASMPMLVVENVTDGNRAYCNLNEGIGKVMRFGAYGEDVLTRHRWMRDVLMPVLS
                     AALGRMERGIDLTAMMAQGITMGDEFHQRNIASSALLMRALAPHIARLDHDKQHIAEV
                     MDFLSVTDQFFLNLAMAYCKAAMDAGAMIRAGSIVTAMTRNGNMFGIRVSGLGERWFT
                     APVNTPQGLFFTGFSQEQANPDMGDSAITETFGIGGAAMIAAPGVTRFVGAGGMEAAR
                     AVSEEMAEIFLERNMQLQIPGWDFQGACLGLDIRRVVETGITPLINTGIAHKEAGIGQ
                     IGAGTVRAPLACFEQALEALAESMGIG"
     gene            complement(19225..20892)
                     /gene="fdrA"
                     /locus_tag="ACHKBL_002324"
     CDS             complement(19225..20892)
                     /gene="fdrA"
                     /locus_tag="ACHKBL_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HFO5819187.1"
                     /translation="MIHAFIKKGCFQDSVSLMIISRKLSESENVDDVSVMMGTPANKA
                     LLDTTGFWHDDFNNATPNDICVAIRSEAADAGIAQAIMQQLEEALKQLAQGSASSQAL
                     TQVRRWDSACQKLPDANLALISVAGEYAAELANQALDRNLNVMMFSDNVTLEDEIQLK
                     TRAREKGLLVMGPDCGTSMIAGTPLAFANVIPEGNIGVIGASGTGIQELCSQIALAGE
                     GITHAIGLGGRDLSREVGGISALTALEMLSADEKSEVLAFVSKPPAEAVRLKIVNAMK
                     ATGKPTVALFLGYTPAVARDENVWFASSLDEAARLACLLSRVTARRNAIAPVSSGFIC
                     GLYTGGTLAAEAAGLLAGHLGVEADDTHQHGMMLDADGHQILDLGDDFYTVGRPHPMI
                     DPTLRNLLIADLGAKPQVRVLLLDVVIGFGATADPAASLVSAWQKACAARSDSQPLYA
                     IATVTGTERDPQCRSQQIATLEDAGIAVVSSLPEATLLAAALIHPLSPATQPHTPSLL
                     ENVAVINIGLRSFALELQSASKPVVHYQWSPVAGGNKKLVRLLERLQ"
BASE COUNT         5128 a         5599 c         5345 g         4942 t
ORIGIN      
        1 tttaatgtgg tgtgcttcgt gacaataaat taataaccaa cacaccggca caaatcaaca
       61 tcatgcctat aatggctggc aggtccagcc gttggccgaa aaatccccat gacagtaagc
      121 taatcaggac aataccgact cctgaccaga tagcataagc aatccctgta ggaatataag
      181 ccagcgtctg agctaataac cagaatgatg cacaataaca aataattgta ccaacagatg
      241 gccataaccg tgtaaaacct tctgaaaact tcattaaggt tgtaccaatg acctctgcaa
      301 gtattgcacc accaagataa atataagggt tcatagcata ttctttcctg ttcaaactgg
      361 agagaattgt actacagttt gaactcaact cacctgtttc atcattgtgt tcccattgat
      421 gttcttttat ataccctcag tacccgtttc atcgcggcac tctggcgaca ctccttaaaa
      481 atcagattcg tgctcacctt tccttcccgt tcttctctgg tagcgaaccg gtaatacacc
      541 gttcgccaga ccttaccatc aacgaccagg attcctgccc gcgccatttt agccgcagcc
      601 tgatttatgc tggttacggt tgcgcctgtt accgcggcaa cgtcctgtgc acagaagctc
      661 ttatgcgtcc ccaggtaatg aataattgcc tctttgcccg tcatacactt gctcctttca
      721 gtccgaactt agctttgatt tctgcgatct tcgccagagc ctgtgcacga tttagaggtc
      781 taccgcccat gacaggaagt tgttttactg gttcaggtat cgcctcacca cggttaattc
      841 gcgcggtcat acaggacagt tcatcggcag ccttgcgccg taattccgcg tcagtcaacg
      901 cattggcccg catgttctga tacaggttgg taaccagcca gtagtgcgcg tttgatttcc
      961 acggataaga ctctgcatcc ggatacagac cacgcttccg gcaatactcg taaaccatat
     1021 caaccagctc gctgacgttt ggcagcccgg cggtaacgga tgcttcttcc cggcaccatg
     1081 caacaaactg cccgggtgat ggcagaaatg gtctattctg ccgacgggca acacgcattc
     1141 cggcgctcac ctgttccatc gtggtgatcc cgttttcccg gaaagccaga acccactggc
     1201 ggcggatttc gttcagttcg ttctggtcac gattagccag gctcgccggg aaagttgcca
     1261 gtaactggct gaatacaccg ttgattatct gcgctacctg ctgtacctgc ggcttttcgt
     1321 cgtactgttc cggcatgttg ttggcgatcc gacgcatctg ctcacggtca aagttaatca
     1381 tctgtgcggc gatgtttttc atagatccac cccgtaaatc cagtctgtgt ttgtcaggtc
     1441 gagttttggt ttgctagctg tcacgcctgc ctgttgcttg ttacggttga tttcgagttg
     1501 ggtccacttg tcgcggagtt tggccggact tagcacgtta ccggaccaga agttgtcctg
     1561 gcatgcccag cggaacagca cgcacatgtc gcggtggtta cgtccgtcac gttcacgcat
     1621 caggcggata tcgttagccc accctgcaaa attcggtttt ctggctgatg gcgcgatggt
     1681 cttcaccatg tcaaacatcc actctgcggc ggtcaggtct tctgctgtcc cccacttgct
     1741 gccgctctga attgcagcat ccggtttcac cacaggaaga tcgttttctg gttggtcaga
     1801 ggattcgcca gaattctcgg acgaaaaagg ttttatattg tcttttgtta gtttgtcttt
     1861 tgtgtttacc tgattcgggt aaacgccttt acctgatttg ggtaaacttt ttttacctga
     1921 ttcaggtaaa tttacctctt tcaggtaaac tttatttttc ttacctgatt cgggtaatgt
     1981 tgaccattca ctgaccacat tattaatgcc ggtattccgc ccgctctgaa taaaaatccc
     2041 acgctttacc agaacacttt ttgcagcaga acacttgtgc ggcaatatcc cggttaattc
     2101 ggaaagttgc tcgttgctaa cccaatccag ttttttatta aagccatatg ttttgcgcat
     2161 gacagccaga aagaccagaa gctggtgctg tgttaatccg gccagcatca cagcttccag
     2221 caactcattt gcaatgcgcg tataaccatc atcgagatct gccacgcgcg gctccttttg
     2281 tgccgcatcc ggcactggaa aattgaatat ctcagcagtg tttgccataa ttcctcccgc
     2341 aatgagtgtg ttacgatttg cacctgaaag tcggttctgt tccagcagac cggctttcgc
     2401 catttctgaa cctgtcatat cgcccccagc atggtagtaa ccatcgccat caatggacca
     2461 gccagatctg ggtccacacg aaacatcgac acaatacctt cactaatttc cttcagtttc
     2521 tggtggcgtg gtgcgttgag aatgacagcc tgttttgcct cactgagttc cttttccatt
     2581 tcagccaacc tagccatgaa gctatcctgc tcaaccaggt aaccgcgata ttccagcggt
     2641 agtaccgcca gaattgccgg ggtcagttca cgcacgttat ttcggtattt ttcagaatcg
     2701 aatttgttat cgaggaagcg gaacagcttc tggcgtgcac ggctgacatc atcagggaaa
     2761 tcgatggtgc cgccgccctg ctcccgatac tcattcacaa tgagtgtggc aacgacatcc
     2821 tgattatcta cagccgacca ggcgcggacg gcatcacgga ttttttcgtg gcctggcacc
     2881 tgttttgttt gagaacgatt tatcaccgca gtcgggctaa atccgctagt ctgttggtat
     2941 gtaagtggtt gcataattga ctcctttagt ttgaattgac tgttaagttg attgcttatt
     3001 gttaaagagc gtgaaatgga aatttaagct gcgttctttt cggtgtgtgg aaacaacttc
     3061 ggaagatccg ggcgaatctg gtatgccttc actactccac cagtagccgt aacaatgctg
     3121 ccgacatgtt caggggatac ctttgctttg ttgtgaagcc acttatagac ggcctgctgt
     3181 gaaacttcgc aagcagcgcc cagtttcttt tgtgaaccaa cgatattgat cgctgttttg
     3241 atagctgggt tcataacaac ctccgtggtt aatttgaatc aagattaaaa ctatggttgt
     3301 ttttagtcaa caaccatttt cgtttgatgg aataaaacct tggttgtaca tttggactat
     3361 gaaaacaaca ctctcagaaa gacttaaaga agccagatta gcgcgaggcc ttacacaaaa
     3421 ggcgcttggg gatttggtcg gggttagcca ggctgctatt cagaaaatcg aaacagggaa
     3481 agctaatcaa acaactaaaa tcgtggagat cgcgaacgct ttgggtgtgc gcgcagaatg
     3541 gttatcttct ggcgttggaa atatgtcaga cagtacagtg caaccaatac aatcaactgt
     3601 cagccattcc aaatacttca agattgacgt tcttgatata gaagtcagtg ctgggccggg
     3661 agtcatcaac cgtgagtttg tagaagttct acgctcggtt gagtactcgt ttgacgatgc
     3721 tcgtcacatg ttcgatggta ggaaggcgga aaatatccgc atcattaacg tgcgtggtga
     3781 cagcatgtca ggaacgatcg aaccaggtga tctgctgttc gttgatatca cagttaaatc
     3841 tttcgacggt gatggtatct atgcgtttct gtacgacgac acagcccatg taaagcgcct
     3901 gcaaatgatg aaggataagc tgctggtcat ctctgataac aaaagctact caccgtggga
     3961 cccgatcgag aaagacgaga tgaaccgggt gttcatcttc ggtaaggtta ttgggagcat
     4021 gccgcagaca tataggaagc atgggtagta ccaattaaaa attatcaact gggcattgtg
     4081 ctcattcagt aaagaactaa ttcctatctt tgctctaggt agtaatatta agccaccgca
     4141 ataatatctt tacctaacgg cgtaagaatc ccggtcaccg tgccgggttt tcttttgccc
     4201 tcccctcatc acacacaccg ttaaaaaaac caccataacc tcgcttcagt tatcgctatg
     4261 cgattcaagt cacaaaataa atccatccta aatacaacca gttatatcta aaacaaccaa
     4321 taaaacaact tttgttgttg acgataaaac aactatagtt ttaaataagt tcatcgcaac
     4381 aacacaacga tacggcaact acctgattca ccgttgcgat gaccgcttag atccgcagtt
     4441 tgaatttcag caggcttcgg ggagtgcgag gggtgaaacg gacgcgtgaa cgtcggtgtg
     4501 accagctgaa attaactcaa catttcatac cttagtcgct tcaacgaggc ggcttagtta
     4561 tgacaaccgg cggccatcca ccgcctgaat acgcgcagaa gtctctatat gttcagcagc
     4621 ccagcttacg ggcaggagtt tttatggttc atcaacatta tggaacgcag accgttaatc
     4681 gaggtgcggt catgccagga atgctggtca aacacaaaga tggtacctgg actgcatcag
     4741 ctaatttacg cggacggctt tatctgcatc gcggcatcga gcgcacttat acccgtgatt
     4801 tgctcgtgga agtttttctc gacggacgcg gtaacggcct gaatcactaa tcccctttcc
     4861 tgttttccta atcagcctgg catttcgcgg gcgatatttt cacagccatt ttcaggaggt
     4921 cagccatgaa cgcttattac attcaggatc gtcttgaggc tcagagctgg gcgcgtcact
     4981 accagcagat cgcccgtgaa gagaaagagg cagaactcgc agacgacatg gaaaaaggcc
     5041 tgccccagca cctgtttgaa tcgctatgca tcgatcattt gcaacgccac ggggccagca
     5101 aaaaagccat tacccgtgcg tttgatgacg atgttgagtt tcaggagcgc atggcagaac
     5161 acatccagta catggttgaa accattgctc accaccaggt tgatattgat tcagaggtat
     5221 aaaacggatg agtacagcac tcgcaacgct ggcagggaag ctggctgaac gtgtcggcat
     5281 ggattctgtc gacccacagg aactgatcac cactcttcgc cagacggcat ttaaaggtga
     5341 tgccagcgat gcgcagttca tcgcattgct gatcgtcgcc aaccagtacg gccttaatcc
     5401 gtggacgaaa gaaatttacg ccttccctga taagcagaac ggcattgttc cggtggtggg
     5461 cgttgatggc tggtcccgca tcatcaatga aaaccagcag tttgatggca tggactttga
     5521 gcaggacaat gaatcctgta catgccggat ttaccgcaag gaccgtaatc atccgatctg
     5581 cgttaccgaa tggatggatg aatgccgccg cgaaccattc aaaacccgcg aaggcagaga
     5641 aatcacgggg ccgtggcagt cgcatcccaa acggatgtta cggcataaag ccatgattca
     5701 gtgtgcccgt ctggccttcg gatttgctgg tatctatgac aaggatgaag ccgagcgcat
     5761 tgtcgaaaat actgcataca ctgcagaacg tcagccagaa cgcgacatca ctccggttaa
     5821 cgatgaaacc atgcaggaga ttaacactct gctgatcgcc ctggataaaa catgggatga
     5881 cgacttattg ccgctctgtt cccagatatt tcgccgcgac attcacgcat cgtcagaact
     5941 gacacaggcc gaagcagtga aagctcttgg attcctgaaa cagaaagcca ctgagcagaa
     6001 ggtggcagca tgataccgga cattatcctg cagcgtaccg ggatcgacgt gagagctgtc
     6061 gaacaggggg atgatgcatg gcacaaatta cggctcggcg tcatcaccgc ttcagaagtt
     6121 cacaacgtga tagcaaagcc ccgctcagga aagaagtggc ctgacatgaa aatgtcctac
     6181 ttccacaccc tgctggctga ggtttgcacc ggtgtggctc cggaagttaa tgctaaggcg
     6241 ctggcctggg gaaaacagta cgagaacgac gccagaaccc tgtttgaatt cacttccggc
     6301 gtgaatatta ctgaatcccc gatcatctat cgcgacgaaa gtatgcgcac cgcctgctcc
     6361 cccgatggtt tatgcagtga cggcaacggc cttgaactga aatgcccgtt tacctcccgg
     6421 gatttcatga aattccggct cggtggtttc gaggcaataa aatcggctta catggcccag
     6481 gtgcagtaca gcatgtgggt gacgcgaaaa gatgcctggt actttgccaa ctatgacccg
     6541 cgcatgaagc gtgaaggcct gcattatgtc gtgattgagc ggaatgaaaa gtacatggcg
     6601 agttttgacg agatggtgcc ggagttcatc gaaaaaatgg acgaggcact ggctgaaatt
     6661 ggttttgtat ttggggagca atggcgatga cgcatcctca cgataatatc cgggtaggcg
     6721 cgatcacttt cgtctactcc gttacaaagc gaggctgggt atttcccggc ctttctgtta
     6781 tcagaaatcc actgaaagca cagcggctgg ctgagaagat aaataataaa cgggaggcgg
     6841 tatgcacaaa gcatctcctg ttgagttaag aacgagtatt gaggtggcac atagccttgc
     6901 tcaaattgga gtcaggtttg tgccaatacc agtagaaaca gacgaagaat ttcatacgtt
     6961 agccacatcc ctttcacaaa agctggaaat gatggtggcg aaagcagaag cagatgagag
     7021 agaccaggta tgacaaccac tgaatgcatt tttctggcag cgggcttcat attctgtgtg
     7081 cttatgcttg ccgacatggg gcttgttcaa tgacacctca gcaagaaaac gcccttcgca
     7141 gcattgcccg tcaggctaat tctgaaatca aaaaagccag acagcatttt ccggataaaa
     7201 acgtcgatga catttgccgt agcgtactaa agaagcaccg cgaaacggta acgctgatgg
     7261 gattcacacc gactcattta agcctggcga tcggcatgtt gaacggcgtc tttaaggaac
     7321 ggtaaacatg aaaagcaaaa tcatcaggga gctacaggct ccttttttat tattcgcatt
     7381 taccctcaag cgtattaacc aacaattcag ggattaatga aagatggcag acatcattga
     7441 ttcagcatca gaaatcgaag aattacagcg caatacagca ataaaaatgc gtcgtctgaa
     7501 ctaccagact gtatccgcaa ctcattgttg tgagtgtggc gatccgatag atgagcgaag
     7561 acgcctggct gttcagggtt gtcggacttg tgcgagttgc caggaggaga tcgaacttaa
     7621 gaacaaacaa tagggactgt gatggcctca aagcagcaaa tttcaacatc gtccaactga
     7681 ggtgtaaaaa tgttcagaat catttttcct aacacctggt acgtcgacca ccacggcact
     7741 ccctgcaaaa tcctgcgttc tacccacaac aaagttcact acatccgaaa aggcagaaca
     7801 tgtatcgcca gcatgttccg ctttaatcat gactttgaac ctgtgaataa agctgatgca
     7861 gatcggatag cagaagagat cgaaacggca gaacacatta agaagttacg tgacatgcgt
     7921 tcaaaaagca gaggtaacca tggaatcata cagcctcaca ctcgatgagg cctgtcagtt
     7981 tcttaagata tccagaccaa ccgccaccaa ctggatacga acaggccgcc tacaggcaac
     8041 acgtaaagat ccaaccaagc caaaatctcc ttacctcaca acacggcaag cctgcattgc
     8101 ggcgcttcag tctccgctgc atactgtcca ggtgagcgcg ggtgatggca taacagagga
     8161 aagaaaatgt cactcttccg cagaaatgaa atatggtatg cctcgtattc gctcccgggc
     8221 gagaaacgaa ttaaggaatc tcttggcaca aaggacaagc ggcaagctca ggagttgcac
     8281 gacaagcgaa aagcagaact ctggcgagta gaaaagctag gggatttacc tgatgtcact
     8341 tttgaagagg cctgcctaag atggcttgag gaaaaagctg ataaaaaatc tctcgattca
     8401 gataaaagcc ggattgagtt ctggcttgaa cattttgagg gtataaggct taaagatatc
     8461 tcggaggcaa agatttactc tgctgtaagc agaatgcata acagaaagac gaaagaaata
     8521 tggaaacaga aagttcaggc cgccatcagg aaaggtaaag aaccgcctgt ttatgaacca
     8581 aagccagtat caactcagac aaaggcaaag catcttgcca tgataaaggc cattctccgt
     8641 gctgcagaac gcgactggaa gtggctggaa aaagcgcctg tcatcaagat accagcggtc
     8701 agaaacaagc gagtcagatg gctggaaaag gaggaagcaa aacgccttat tgatgagtgc
     8761 cccgaaccac tgaaatctgt cgtcaagttt gcgctggcaa ctggtctgag aaagtcgaac
     8821 atcataaatc tggaatggca acaaatcgac atgcagcgac gagttgcctg ggtgaatcca
     8881 gaagagagca aatcaaaccg cgccattggt gtggcgctga acgataccgc ctgtaaagtg
     8941 ttgcgtgatc aaataggcaa gcatcacaaa tgggtgtttg tacataccaa ggcggctaag
     9001 cgagcagatg gaacatcaac gcctgcggtc aggaagatgc gcatcgacag caagacatca
     9061 tggctatcag cttgtcgtcg tgcaggaatt gaagatttcc gtttccatga cctcagacac
     9121 acctgggcaa gctggctgat tcagtcaggc gtcccattat cagtgcttca ggaaatgggc
     9181 ggatgggagt ccatagaaat ggttcgtagg tatgctcacc ttgcgcctaa tcatttgaca
     9241 gagcatgcga ggaaaataga cgacattttt ggtgataatg tcccaaatat gtcccactct
     9301 ggaattatgg aggatataaa gaaggcgtaa ctgattgaat tgtaatggcg cgccctgcag
     9361 gattcgaacc tgcggcccac gacttagaag gtcgttgctc tatccaactg agctaagggc
     9421 gcattgatac cacggtgtgc tgtaatcgcg tgaattatac ggtcaaccct tgctgagtca
     9481 atggcttttg aaccagttgc tgaacaagtg tacgaccgca tcagatgtag ctcactatgg
     9541 cggcaaacca acgacggtta tcatgatagg tgtgaatcag ccaggtacag aaggttttac
     9601 ttttcagaaa acgaaaactg acagcgcgcc acgcttctga caaaatagac gcatcccctt
     9661 cgacctacgt aacagatgga atcctctctc tgatggcagc aaagattatt gacggtaaaa
     9721 cgattgcgca gcaggtgcgc tctgaagttg ctcaaaaagt tcaggcgcgt attgcagccg
     9781 gactgcgggc accaggactg gccgttgtgc tggtgggtag taaccctgca tcgcaaattt
     9841 atgtcgcaag caaacgcaag gcttgtgaag aagtcgggtt cgtctcccgc tcttatgacc
     9901 tcccggaaac caccagcgaa gcggagctgc tggagcttat cgatgcgctg aatgccgaca
     9961 acaccatcga tggcattctg gttcaactgc cgttaccggc gggtattgat aacgtcaaag
    10021 tgctggaacg tatacacccg gacaaagacg tggacggttt ccatccttat aacgtcggtc
    10081 gcctgtgcca gcgcgcgccg cgtctgcgtc cctgcacccc gcgcggtatt gtcacgctgc
    10141 ttgagcgtta caacattgat accttcggcc tcaacgccgt ggtgattggc gcatcgaata
    10201 tcgttggccg cccgatgagc atggaactgc tactggcagg ttgcaccacc accgtgactc
    10261 accgtttcac taaaaatctg cgtcaccacg tagaaaatgc cgatctactg atcgttgccg
    10321 tcggcaagcc gggctttatt cctggtgact ggatcaaaga aggcgcaatt gtgattgatg
    10381 tcggcatcaa ccgtctggaa aatggcaaag ttgtgggcga cgtcgtgttt gaagacgcgg
    10441 ctaaacgcgc ctcatacatt acgcctgttc cgggcggcgt tggcccgatg acggttgcca
    10501 cgctgattga aaacacgcta caggcgtgcg ttgaatatca tgatccacag ggtgagtaac
    10561 atggcgacat tttctttagg taaacatccg cacgttgagc tgtgcgactt gctgaaactg
    10621 gaaggctgga gcgaaagcgg cgcgcaggcg aaaatcgcga ttgccgaagg ccaggtgaaa
    10681 gtcgacggtg cggttgaaac gcgcaaacgc tgcaaaatcg tcgccgggca gacagtgagt
    10741 tttgcaggtc acagcgtaca ggttgttgcc tgatcccttt cccggcctgt aacaacaggc
    10801 cggttctctt ttcagacttt tgccctctac gccccgttca tccatgctga tagcatcgac
    10861 attttgccga agcgaccatc atgccaaccg ttattacgca cgccgccgtt cccctttgca
    10921 ttggtttagg gttgggttcg aaagtcatcc ctccgcgtct gttatttgcc ggaatcgtcc
    10981 tggcgatgct gcccgatgcc gacgtgttgt cgtttaaatt tggcgttgct tacggcaatg
    11041 tttttggtca tcgcgggttt acccattcgc tggtgtttgc gtttgttgtc ccgctgttat
    11101 gtgtgctgat tgggcgtcga tggttcaggg ctgggctgat tcgctgctgg ctgtttttaa
    11161 ccgtctcgtt gctatcgcac agcttgctgg attcggtaac cactggcggt aaaggggttg
    11221 gctggctgtg gccgtggtca gatgaacgct ttttcgctcc ctggcaggtg attaaagtcg
    11281 cgccgtttgc attgtctcgt tacaccacgc cgtacgggca taaggtgatt atttctgaat
    11341 tgatgtgggt gtggttgccg gggatgctac tgatgggaat gttgtggtgg cgcagacgat
    11401 aaccggatgc gaaaactcgc atccggcaat agcgcaatta cttacgacgc caggtggtcc
    11461 cttgcgggcc atcttccagc acgatcccca tctcgttaag acggtcacgc gccgcatctg
    11521 ccgccgccca gtctttcgct ttacgggcat ccagacgctg ttgaattaac gcttcaatct
    11581 cagccacttc actgtcgtct gcctgcgcgc cgctttgcag gaacgcttcc ggttcttgct
    11641 ccagcaggcc caatacagcg gaaagtttac gcaggtgaga cgccattgca ttcgccgctg
    11701 ccatatcttc tgctttcaga cggttcactt cacgcgccat atcaaacagt acggaatagg
    11761 cttccggggt gttgaaatcg tcgttcatcg cctcaataaa gcgcgcttca aacgcttcgc
    11821 caccggcagg agcaacggtt ttgtctgtgc cgcgcaaggc agtatagaga cgttccagcg
    11881 ccgcacgcgc ctgcttcagg ttctcttcgc tgtagttcaa ctgactgcga tagtgacccg
    11941 acatcaggaa gtaacgcaca gtttccgcat cgtagtattt cagcacatca cgcacggtga
    12001 agaagttacc cagcgattta gacatcttct cgcggtcaac catcaccatc cccgagtgca
    12061 tccagtagtt cacatactga ccatcatggg cacaggtgga ctgggcaatt tcgttctcgt
    12121 ggtgcgggaa catcaggtca gaaccgccgc cgtggatatc aaagtggtta cccaactgtt
    12181 tgcagttcat tgccgaacac tcaatgtgcc agccaggacg gcccgcgccc cacggagacg
    12241 gccagctcgg ttcgccctct ttcgacatct tccacagaac gaaatccatc gggttgcgtt
    12301 tgtcgtcgac cacgtcaacg cgcgcgcctg cctgcagctg atcgagatcc tgacgcgaca
    12361 gcacgccata agttggatcg gtcgggacgt cgaacatcac gtcgccgttg tccgccacat
    12421 aagcgtgacc tttggcgatc agttgttcag tgagttcaat aatttcggcg atatggtgcg
    12481 tcgcgcgcgg ctccatatcc gggcgcagaa tgttcaaagc atcaaaatct ttgtgcattt
    12541 cggcgatcat gcgatccacc agcgccacaa agctttcgcc attttcattg gcgcgtttga
    12601 tgattttgtc gtcgatatca gtaatgttgc gcacatactt cagcttatag ccgaggaaac
    12661 gcagatagcg cgcaaccacg tcaaaggcaa caaaggtacg cccgtgaccg atatgacaga
    12721 gatcgtaaac ggtgattcca cacacgtaca tgccgacttc cccggcgtga ataggcttaa
    12781 attcctcttt ttggcgtgtc agagtattga agatttttag catcgaagat tccgtttaga
    12841 catgtgtggg taattgagtt gcgtataata cccatatttc ccgcgcgaat cagcatacat
    12901 tgcgtgatga tccgatcgtg cggttatgct ataacaccac cctatatatg acccgaaccg
    12961 ggttgaagca ccaatcaaac ggaacaggat gcaaaaatgg ttactttcca caccaatcac
    13021 ggcgatattg tcatcaaaac ttttgacgat aaagcacctg aaacagttaa aaacttcctg
    13081 gactactgcc gcgaaggttt ttacaacaac accattttcc accgtgtaat caacggcttt
    13141 atgattcagg gcggcggttt tgaaccgggc atgaagcaaa aaaccaccaa agacccgatc
    13201 aaaaacgaag ctaacaacgg tctgaaaaat acccgtggta cactggcaat ggcccgtact
    13261 caggcaccgc actctgcaac tgcacagttc ttcatcaacg tggttgataa cgacttcctg
    13321 aacttctctg gcgaaagcct gcaaggttgg ggttactgcg tgtttgctga agtggttgaa
    13381 ggcatggacg tggtagacaa aatcaaaggt gttgcaaccg gtcgtagcgg tatgcaccag
    13441 gacgtgccaa aagaagacgt tatcattgaa agcgtgaccg ttagcgagta atcgtggcga
    13501 cactctttat tgcagatctt catctctgcg cggaagaacc ggcgatcacc gccggttttc
    13561 tgcgtttttt agcgggggaa gcccgcaagg ccgacgcgct gtatattctt ggcgatctgt
    13621 ttgaagcatg gattggcgac gacgatccca acccactcca tcgccagatg gcggcagcga
    13681 tcaaagcggt gtccgattcc ggcgttccct gttatttcat tcatggcaac cgtgattttc
    13741 tgctcggcaa acgctttgcc cgtgaaagcg gcatgacgtt attgccggaa gaaaaggtgc
    13801 tcgaacttta tggtcgccgg gtgttgatta tgcatggcga cacgctgtgc accgatgacg
    13861 ccggttatca ggcttttcgc gccaaagtcc acaaaccctg gctgcaaacg ctattcctcg
    13921 ccctgccgtt gtttgtgcgc aaacgcattg ccgcgcgaat gcgcgcgaac agcaaagaag
    13981 ccaacagcag taaatcgctg gcgatcatgg acgttaacca aaacgcggtg gtcagtgcga
    14041 tggaaaaaca tcaggtgcaa tggctgattc acgggcatac ccatcgcccg gcagtacatg
    14101 aacttatcgc caatcagcaa cctgctttcc gcgtcgtgct gggtgcctgg catactgaag
    14161 gttcgatggt gaaagtcacg gcggatgacg ttgagttgat ccattttcca ttttaaaaaa
    14221 cgcgcaactt tgctgatttc ataaccgcgc aaccgttttc cttgctctct ttccgtgcta
    14281 ttctctgtgc cctctaaagc cgagagttgt gcaccacagg agttttaaga cgcatgtctt
    14341 cccgcaataa tccggcgcgt gtcgccatcg tgatggggtc caaaagcgac tgggctacca
    14401 tgcagttcgc cgccgaaatc ttcgaaatcc tgaatgtccc acaccacgtt gaagtggttt
    14461 ctgctcaccg cacccccgat aaactgttca gcttcgccga aagtgccgaa gagaacggtt
    14521 atcaggtgat tattgcgggc gcaggcggcg cagcgcacct gccaggcatg attgccgcca
    14581 aaacgctggt gccagtgctg ggcgtgccag tacagagcgc cgcactgagc ggtgtcgata
    14641 gcctctactc catcgtacaa atgccgcgcg gcattccggt gggtacgctg gcgattggta
    14701 aagctggcgc ggcaaacgcg gcgttactgg cagcacaaat tcttgcgact catgataaag
    14761 aactgcacca gcgtctgaat gactggcgca aagcccagac cgacgaagtg ctggaaaacc
    14821 cggacccgcg aggtgcggca tgaaacaggt ttgcgtcctc ggtaacgggc agttaggccg
    14881 tatgctgcgt caggcaggcg aaccgttagg cattgctgtc tggccggtcg ggctggacgc
    14941 tgaaccggcg gcggtgcctg ttcaacaaag tgtgattacc gctgagatcg aacgctggcc
    15001 ggaaaccgca ttaacccgcg agctggcgcg ccatccggcc tttgtgaacc gcgatgtgtt
    15061 cccgattatt gccgaccgtc tgactcagaa gcagcttttc gataagctcc acctgccgac
    15121 cgcgccgtgg cagttacttg ccgatcgcag cgagtggcct gcggtgtttg atcgtttagg
    15181 tgaactggcg attgttaagc gtcgcactgg tggctatgac ggtcgcggtc aatggcgttt
    15241 acgcgcaaat gaaaccgaac agttaccggc agagtgttac ggcgaatgta ttgtcgagca
    15301 gggcattaac ttctctggtg aagtgtcgct ggttggcgcg cgcggttttg atggcagcac
    15361 cgtgttttat ccgctgacgc ataacctgca tcaggacggt attttgcgca ccagcgtcgc
    15421 tttcccgcag gccaatgcgc agcagcaagc gcaagccgaa gagatgctgt cggcgattat
    15481 gcaggagctg ggctatgtag gcgtgatggc aatggagtgt tttgtcaccc cgcaaggtct
    15541 gctgatcaac gaactggctc cgcgtgtgca taacagcggt cactggacac aaaacggtgc
    15601 cagcatcagc cagtttgagc tgcatctgcg ggcgattacc gatctgccgt taccgcaacc
    15661 ggtagtgaat agtccgtcgg tgatgatcaa cctgattggt agcgatgtga attatgactg
    15721 gctgaaactg ccgctggtgc atctgcactg gtacgacaaa gaagtccgtc cggggcgtaa
    15781 agtggggcat ctgaatttga ccgacagcga cacatcgcgt ctgaccgcga cgctggaagc
    15841 cttaatcccg ctgctgccgc cagagtatgc cagcggcgtg atgtgggcgc agagcaagtt
    15901 cagttaattg atgctcattt tcttgccgga tgcggcgtga acgccttatc cggcccaccg
    15961 ctccaacacc gactgtaggc ctgataagac gcgccagcat cgcatcaggc atggtgtgcc
    16021 gattgcagga tgcggcgtga acgccttatc cggcctaccg ttccaacacc gactgtaggc
    16081 ctgataagat gcgccagcat cgcatcaggc atggtgtgcc gattgccgga tgcggcgtga
    16141 acgccttatc cggcctaccg ttccgacacc gcctgtagac ctgataagac gcgccagcat
    16201 cgcatcaggc tagatgtgcc aattgcctgg tgcccatcac tacagcgaaa tacaagtccc
    16261 cgcttcgccc gccagcgtct cttcaatccg cgataacgcc ccaatccacg cgggtttgcc
    16321 acggcttctg acatagccac tcaccgcagt caccttcggc cccatcgaac catcggcttt
    16381 ggcaaatggc gctaactcat ccggtgtggc atggcgaatg gcacgttgct gcggcgttcc
    16441 ccagttttca tataccgcat cagcatcggt gaggatcacc agtccatcgg cattaatctg
    16501 ctcggcgagc aacgcagcgg cgagatcttt atcaattacc gcttcactcc ctgccccgtc
    16561 tttcgtcaca ggcacaccgc cgccgccact gcaaatcacc acatgcccct ctttgagcaa
    16621 caactcgatg gcttcgctgt cgagaatttt acgcggttgc ggagacgcca ccacccggcg
    16681 taaatattta ccatcacgtt tcatctgcca gccataagcc gcttccagtg cctcttgttc
    16741 ttctggctga taaaccggac cgataaactt ctctggctgc aaaaacgccg gatcatcagg
    16801 cgaaacttca atgcgcgtca gcactgtcgt gacaggcggc atctgcggct gtgcgctcaa
    16861 gctctgcgcc agcatatagc caatcatccc ctggctttcc gccaccagca catccagcgg
    16921 atacggttct acctctttcc acgccagatt ctgtaacgcc agcagcccca cctgcggccc
    16981 gttgccgtga acaatcgcca gccgataaga acgggccagg cgcgccagtg cgggtacagc
    17041 actggcaatg ttgcgatatt gattttctgc cgtcagcgcc tcaccgcgct ggagtaaggc
    17101 gttgccccca agagccacaa ccagtgtttt catgattttc cttgtaataa ttgttggcca
    17161 agccagaacc ccagcagcgt atctgcccct gacgtatgcc ccagcgccag cagcgaatca
    17221 atcgcaacag cggtacgttt cgggcaactc agagcatgaa taaagtgcag gagtggcgag
    17281 gcgaagtatc cttgcgcggc ataacgtaaa taactgacgc tcaccgctgt ggtaacgagt
    17341 tgaagattgt cggaacaggc aaaaaacggg cgaccggagc gcgcatctaa agcgccataa
    17401 taccaggccg ccagcagcat tccgctcagc gtgtcatcat gacttggcgt taatcctggg
    17461 cctttaccca gccagtgccg ccagtcggtc ttaacgccat tgagcgcggc ctgaaaacag
    17521 tgacgaaact ggcgtaattc agcaggaagc ggatcgcctg ccgccaacgc cagtggcccg
    17581 aaaagcccgg tttcctccgc gcgttgcatc catgccgctg caagcggttg aggatgcgca
    17641 ggcggcgtaa tacgcagcaa acaataacgc tgtggctgtt taaccgtgaa acgtccgagg
    17701 cgaatccctt gagccataac ctgtggtcgt tcattgccgc ataatccacc gcataacgca
    17761 tcgaattgcg cgcggcgaag cacccatcct ccggggccga aaccactacc ctgacgatgc
    17821 agcgttaaca gttcgccgct ttccgtcatc aggttaatcg cccgccgcca cactcccgct
    17881 aaccgccagg actggcgata attcggtgcg ctactactgg caagcagagg atggatgatc
    17941 gtcattgtgc gttcctcaac caatccccat gctttcagcc agtgcttcca gcgcctgctc
    18001 aaagcacgcc agcggtgccc gcacggtgcc tgcgccaatc tgcccgatcc ccgcctcttt
    18061 atgggcgatt ccggtattga tgagcggcgt aatgccggtt tctaccacgc gacgaatatc
    18121 cagccccagg cacgcgccct ggaaatccca gccggggatc tgcaactgca tattgcgttc
    18181 aaggaagatt tccgccatct cttcagatac cgctctggcc gcttccatgc cacccgcacc
    18241 gacaaagcgc gttacgccag gcgctgcgat catcgccgca ccgccaatac caaaggtttc
    18301 ggtaattgcg ctatcgccca tatccgggtt cgcctgctcc tgcgagaagc cggtgaaaaa
    18361 cagcccttgg ggagtgttta caggcgcagt aaaccagcgt tcgcccagcc cgcttacccg
    18421 aatcccgaac atattgccgt tgcgggtcat agccgtgacg atgctgcctg cgcggatcat
    18481 cgcaccagca tccatcgccg ccttgcagta agccatcgcg aggttaagga agaactgatc
    18541 ggtcacgctg aggaaatcca tcacttccgc gatgtgctgt ttatcatgat cgaggcgagc
    18601 aatgtgtggg gccagcgcac gcattaacag tgctgaggaa gcaatattgc gttgatggaa
    18661 ctcatcgccc atggtaatgc cctgcgccat catcgcggtg agatcgatac cgcgctccat
    18721 gcgccccagc gccgcgctta atactggcat taacacatcg cgcatccagc gatggcgagt
    18781 cagaacatct tcgccgtaag cgccaaaacg catcactttg ccgatacctt cgttgaggtt
    18841 gcagtacgcc cggttgccgt cggtcacgtt ctcgaccacc agcatcggca tactggcaga
    18901 agtaatgccg cccattggcc cgacggcatt cacatggtga caaggaatga agttcacttc
    18961 cccctgatcc agcattgcca gcgcctgcgc ttcatctttc gcccagcctt caaacagaca
    19021 tgcgcccaca catgcccctt tcatgggtcc ggtcatctcc tgccagcgca ttggcggccc
    19081 ggcgtgaagc agtgttttgc cctcgtttag ttcgctgata agtgaagaag ccggttgcac
    19141 atccagccag tgtggacgag cgcgacgaat ttgttcgata accgcagcat tggcttgcgc
    19201 cactgatgta aacatgggaa ccccttattg caaacgttct aataaacgaa ccagtttttt
    19261 attgccaccg gcgactggcg accattggta atgcacaacc ggtttgctgg cgctttgtag
    19321 ctccagcgca aagctgcgta atccgatgtt aatcacggcg acgttttcca gtaatgacgg
    19381 tgtatgtggc tgtgtggcag gcgagagcgg atgaattaac gccgccgcca gcaaggtggc
    19441 ttccggcagc gaactcacga ccgcaatccc cgcatcttcc agcgtggcga tttgctgcga
    19501 gcggcattgc gggtcacgtt cagtgcctgt caccgtggca atggcgtaca gcggttggct
    19561 gtcagaacgc gcggcacagg ctttttgcca ggcgctcacc agcgaggcgg caggatcggc
    19621 ggtcgcaccg aagccaatca cgacatcaag cagcaacacg cgcacttgcg gtttagcgcc
    19681 gagatcggca attaacaggt tgcgtaaggt cggatcgatc atcggatggg gacgcccgac
    19741 ggtgtaaaaa tcatcgccca ggtcgaggat ctgatgtccg tcggcgtcca gcatcatgcc
    19801 atgttgatgg gtatcgtcgg cttccacgcc aaggtgtccg gcaagtaatc ccgccgcttc
    19861 agcagccagc gtaccgccgg tatacaagcc gcaaataaat ccgctgctga caggcgctat
    19921 tgcgttacgt cgcgcagtga cgcgtgaaag cagacaagcc aggcgtgcgg cctcatccag
    19981 cgaggaggca aaccagacat tctcgtcgcg ggccaccgcc ggggtataac ctaaaaacag
    20041 cgccaccgtc ggtttgccag ttgctttcat ggcattaaca attttcagac gcaccgcttc
    20101 ggcaggtggt tttgaaacaa atgccagcac ttcgcttttc tcgtctgcac tgagcatttc
    20161 cagcgccgtc agcgcactga tgccgcccac ttcacggctg aggtcgcgcc cgccaaggcc
    20221 aatcgcgtga gtaattccct cccctgccag cgcaatctgt gagcacagct cctgaatccc
    20281 ggtaccggaa gcgccaatga cgccaatgtt gccttccggt atcacgttag caaaagccag
    20341 cggcgtgccg gcaatcatcg acgtaccgca gtccggcccc atcaccagca agcctttttc
    20401 ccgcgcgcgg gttttaagtt ggatttcatc ttccagcgtg acgttatcgg agaacatcat
    20461 cacgttgagg ttgcgatcca gcgcctggtt cgccagctcc gccgcatact caccagccac
    20521 tgaaatcagc gccagattgg catcgggtaa tttctgacag gcactgtccc agcgacgcac
    20581 ctgcgtcaac gcctggctgc tggctgaccc ctgcgccagt tgttttagcg cctcttcaag
    20641 ctgctgcata atcgcctgcg cgatccccgc atccgccgct tcgctacgaa ttgccacgca
    20701 aatatcgttc ggcgtggcgt tattaaaatc gtcatgccag aaacctgtgg tatctaataa
    20761 cgctttattg gcgggcgtac ccatcattac ggaaacatca tcaacatttt ctgattcgct
    20821 gagttttcgt gaaataatca ttaaactgac cgaatcctga aaacaccctt ttttaataaa
    20881 ggcgtggatc ataacaactc cttaaaaatt gcacccatgg caattgcacg agcagtgtta
    20941 atgtggcgat tacggtatcg atgacaacga gtaaataagg tgatgaatat cacacaggga
    21001 ttattaacaa aaga
//
LOCUS       DAZFLD010000074        20542 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.074, whole genome shotgun sequence.
ACCESSION   DAZFLD010000074 DAZFLD010000000
VERSION     DAZFLD010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20542
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.074"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(29..4489)
                     /gene="mukB"
                     /locus_tag="ACHKBL_002325"
     CDS             complement(29..4489)
                     /gene="mukB"
                     /locus_tag="ACHKBL_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HFO5819188.1"
                     /translation="MIERGKFRSLTLINWNGFFARTFDLDELVTTLSGGNGAGKSTTM
                     AAFVTALIPDLTLLHFRNTTEAGATSGSRDKGLHGKLKAGVCYSMLDTINSRHQRVVV
                     GVRLQQVAGRDRKVDIKPFAIQGLPMSVQPTQLVTETLNERQARVLPLNELKDKLEAM
                     EGVQFKQFNSITDYHSLMFDLGIIARRLRSASDRSKFYRLIEASLYGGISSAITRSLR
                     DYLLPENSGVRKAFQDMEAALRENRMTLEAIRVTQSDRDLFKHLISEATNYVAADYMR
                     HANERRVHLDKALEFRRELHTSRQQLAAEQYKHVDMARELAEHNGAEGDLEADYQAAS
                     DHLNLVQTALRQQEKIERYEADLDELQIRLEEQNEVVAEAIERQEENEARAEAAELEV
                     DELKSQLADYQQALDVQQTRAIQYNQAIAALNRAKELCHLPDLTADSAAEWLETFQAK
                     ELEATEKMLSLEQKMSMAQTAHSQFEQAYQLVVAINGPLARNEAWDVARELLREGVDQ
                     RHLAEQVQPLRMRLSELEQRLREQQEAERLLADFCKRQGKNFDIDELEALHQELEARI
                     ASLSDSVSNAREERMALRQEQEQLQSRIQSLMQRAPVWLAAQNSLNQLSEQCGEEFTS
                     SQDVTEYLQQLLEREREAIVERDEVGARKNAVDEEIERLSQPGGSEDQRLNALAERFG
                     GVLLSEIYDDVSLEDAPYFSALYGPSRHAIVVPDLSQVTEHLEGLTDCPEDLYLIEGD
                     PQSFDDSVFSVDELEKAVVVKIADRQWRYSRFPEVPLFGRAARESRIESLHAEREVLS
                     ERFATLSFDVQKTQRLHQAFSRFIGSHLAVAFESDPEAEIRQLNSRRVELERALSNHE
                     NDNQQQRIQFEQAKEGVTALNRILPRLNLLADDSLADRVDEIRERLDEAQEAARFVQQ
                     FGNQLAKLEPIVSVLQSDPEQFEQLKEDYAYSQQMQRDARQQAFALTEVVQRRAHFSY
                     SDSAEMLSGNSDLNEKLRERLEQAEAERTRAREALRGHAAQLSQYNQVLASLKSSYDT
                     KKELLNDLQRELQDIGVRADSGAEERARIRRDELHAQLSNNRSRRNQLEKALTFCEAE
                     MDNLTRKLRKLERDYFEMREQVVTAKAGWCAVMRMVKDNGVERRLHRRELAYLSADDL
                     RSMSDKALGALRLAVADNEHLRDVLRMSEDPKRPERKIQFFVAVYQHLRERIRQDIIR
                     TDDPVEAIEQMEIELSRLTEELTSREQKLAISSRSVANIIRKTIQREQNRIRMLNQGL
                     QNVSFGQVNSVRLNVNVRETHAMLLDVLSEQHEQHQDLFNSNRLTFSEALAKLYQRLN
                     PQIDMGQRTPQTIGEELLDYRNYLEMEVEVNRGSDGWLRAESGALSTGEAIGTGMSIL
                     VMVVQSWEDESRRLRGKDISPCRLLFLDEAARLDARSIATLFELCERLQMQLIIAAPE
                     NISPEKGTTYKLVRKVFQNTEHVHVVGLRGFAPQLPETLPGSDEAPYQAS"
     gene            complement(4489..5193)
                     /gene="mukE"
                     /locus_tag="ACHKBL_002326"
     CDS             complement(4489..5193)
                     /gene="mukE"
                     /locus_tag="ACHKBL_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HFO5819189.1"
                     /translation="MSSTNIEQVMPVKLAQALANPLFPALDSALRSGRHIGLDELDNH
                     AFLMDFQEYLEEFYARYNVELIRAPEGFFYLRPRSTTLIPRSVLSELDMMVGKILCYL
                     YLSPERLANEGIFTQQELYDELLTLADEAKLLKLVNNRSTGSDVDRQKLQEKVRSSLN
                     RLRRLGMVWFMGHDSSKFRITESVFRFGADVRAGDDPREAQRRLIRDGEAMPIENHLQ
                     LNDETEESQPDSGEEE"
     gene            complement(5174..6496)
                     /gene="mukF"
                     /locus_tag="ACHKBL_002327"
     CDS             complement(5174..6496)
                     /gene="mukF"
                     /locus_tag="ACHKBL_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HFO5819190.1"
                     /translation="MSEFSQTVPELVAWARKNDFSISLPVDRLSFLLAVATLNGERLD
                     GEMSEGELVDAFRHVSDAFEQTSETIGVRANNAINDMVRQRLLNRFTSEQAEGNAIYR
                     LTPLGIGITDYYIRQREFSTLRLSMQLSIVAGELKRAADAAEEGGDEFHWHRNVYAPL
                     KYSVAEIFDSIDLTQRLMDEQQQQVKDDIAQLLNKDWRAAISSCELLLSETSGTLREL
                     QDTLEAAGDKLQANLLRIQDATMTHDDLHFVDRLVFDLQSKLDRIISWGQQSIDLWIG
                     YDRHVHKFIRTAIDMDKNRVFAQRLRQSVQTYFDEPWALTYANADRLLDMRDEEMVLR
                     DEEVTGELPEDLEYEEFNEIREQLAAIIEEQLAVYKTRQVPLDLGLVVREYLSQYPRA
                     RHFDVARIVIDQAVRLGVAQADFTGLPAKWQPINDYGAKVQAHVIDKY"
     gene            complement(6493..7278)
                     /gene="cmoM"
                     /locus_tag="ACHKBL_002328"
     CDS             complement(6493..7278)
                     /gene="cmoM"
                     /locus_tag="ACHKBL_002328"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine 5-oxyacetic acid(34)
                     methyltransferase CmoM"
                     /protein_id="HFO5819191.1"
                     /translation="MQDRNFDDIAEKFSRNIYGTTKGQLRQAILWQDLDRVLAEMGPQ
                     KLRVLDAGGGEGQTAIKMAERGHQVILCDLSAQMIDRAKQAAEAKGVSDNMQFIHCAA
                     QDVASHLETPVDLILFHAVLEWVADPRSVLQTLWSVLRPGGVLSLMFYNAHGLLMHNM
                     VAGNFDYVQAGMPKKKKRTLSPDYPRDPAQVYLWLEEAGWQIMGKTGVRVFHDYLREK
                     HQQRDCYEALLELETRYCRQEPYITLGRYIHVTARKPQSKDKV"
     gene            7414..8193
                     /gene="elyC"
                     /locus_tag="ACHKBL_002329"
     CDS             7414..8193
                     /gene="elyC"
                     /locus_tag="ACHKBL_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope biogenesis factor ElyC"
                     /protein_id="HFO5819192.1"
                     /translation="MLFTLKKVIGNMLLPLPLMLLIIGAGLALLWFSRFQKTGKIFIS
                     IGWLALLLLSLQPVADRLLRPIESTYPTWNNSQKVDYIVVLGGGYTWNPQWAPSSNLI
                     NNSLPRLNEGIRLWRENPGSKLIFTGGVAKTNTVSTAEVGARVAQSLGVPREQIITLD
                     LPKDTEEEAAAVKQAIGDAPFLLVTSASHLPRAMIFFQQEGLNPLPAPANQLAIDSPL
                     NPWERAIPSPVWLMHSDRVGYETLGRIWQWLKSSSGEPRQE"
     gene            complement(8170..9063)
                     /locus_tag="ACHKBL_002330"
     CDS             complement(8170..9063)
                     /locus_tag="ACHKBL_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbJ family phosphotransferase"
                     /protein_id="HFO5819193.1"
                     /translation="MEQLRAELSHLLGEKLSRIECVNEKADTALWALYDSQGNPMPLM
                     ARSFSTPGKARQLAWKTTMLARSGTVRMPTIYGVMTHEEHPGPDVLLLERMRGVSVEA
                     PARTPERWEQLKDQIVEALLAWHRQDSRGCVGAVDNTQENFWPSWYRQHVEVLWTTLN
                     QFNNTGLTMQDKRILFRTRECLPALFEGFNDNCVLIHGNFCLRSMLKDSRSDQLLAMV
                     GPGLMLWAPREYELFRLMDTSLAEDLLWSYLQRAPVAESFIWRRWLYVLWDEVAQLVN
                     TGRFSRRNFDLASKSLLPWLA"
     gene            complement(9217..9963)
                     /gene="kdsB"
                     /locus_tag="ACHKBL_002331"
     CDS             complement(9217..9963)
                     /gene="kdsB"
                     /locus_tag="ACHKBL_002331"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HFO5819194.1"
                     /translation="MSFVVIIPARYASTRLPGKPLVDINGKPMIVHVLERARESGAER
                     IIVATDHEDVARAVEAAGGEVCMTRADHQSGTERLAEVVEKCAFSDDTVIVNVQGDEP
                     MIPATIIRQVADNLAQRQVGMATLAVPIHNAEEAFNPNAVKVVLDAEGYALYFSRATI
                     PWDRDRFAKGLETVGDNFLRHLGIYGYRAGFIRRYVNWQPSPLEHIEMLEQLRVLWYG
                     EKIHVAVAQEVPGTGVDTPEDLERVRAEMR"
     gene            complement(9960..10142)
                     /gene="ycaR"
                     /locus_tag="ACHKBL_002332"
     CDS             complement(9960..10142)
                     /gene="ycaR"
                     /locus_tag="ACHKBL_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcaR"
                     /protein_id="HFO5819195.1"
                     /translation="MDHRLLEIIACPVCNGKLWYNQEKQELICKLDNLAFPLRDGIPV
                     LLETEARVLTADESKS"
     gene            complement(10194..11426)
                     /gene="ycaQ"
                     /locus_tag="ACHKBL_002333"
     CDS             complement(10194..11426)
                     /gene="ycaQ"
                     /locus_tag="ACHKBL_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ"
                     /protein_id="HFO5819196.1"
                     /translation="MSLPHLSLADARNLHLAAQGLLNKPRRRASLGDIPATISRMSLL
                     QIDTINIVARSPYLVLFSRLGNYPAQWLDESLARGELMEYWAHEACFMPRSDFRLIRH
                     RMLAPEKMGWKYKDAWMQEHAAEIAQLIQHIHDKGPVRSADFEHPRKGASGWWEWKPH
                     KRHLEGLFTAGKVMVIERRNFQRVYDLTHRVMPEWNDERDLVSQAEAEIIMLDNSARS
                     LGIFREQWLADYYRLKRPALAAWREARAEQQQIIAVHVEKLGNLWLHADLLPLLERAL
                     AGKLTATHSAVLSPFDPVVWDRKRAEQLFDFSYRLECYTPAPKRQYGYFVLPLLHRGQ
                     LVGRMDAKMHRQTGILEVISLWLQEGIKPTTTLQKGLRQAITDFANWQQATRVTLGRC
                     PQGLFTDCRAGWEIDPVA"
     gene            complement(11463..12449)
                     /gene="lpxK"
                     /locus_tag="ACHKBL_002334"
     CDS             complement(11463..12449)
                     /gene="lpxK"
                     /locus_tag="ACHKBL_002334"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HFO5819197.1"
                     /translation="MIEKIWSGESPLWRLLLPLSWLYGLVSGAIRLCYKLKLKRAWRA
                     PVPVVVVGNLTAGGNGKTPVVVWLVEQLQQRGIRVGVVSRGYGGKAESYPLLLSADTT
                     TAQAGDEPVLIYQRTGAPVAVSPVRSDAVKAILAQHPDVQIIVTDDGLQHYRLARDVE
                     IVVIDGVRRFGNGWWLPAGPMRERAGRLKSVDAVIVNGGVPRSGEIPMHLLPGQAVNL
                     RTGTRCDVAQLEHVVAMAGIGHPPRFFATLKMCGVQPEKCVPLADHQSLNHADVSALV
                     NAGQTLVMTEKDAVKCRAFAEGNWWYLPVDAQLSGDEPAKLLAQLTSLASGN"
     gene            complement(12446..14194)
                     /gene="msbA"
                     /locus_tag="ACHKBL_002335"
     CDS             complement(12446..14194)
                     /gene="msbA"
                     /locus_tag="ACHKBL_002335"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0034040 - ATPase-coupled lipid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HFO5819198.1"
                     /translation="MHNDKDLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFM
                     LSLLKPLLDDGFGKTDRSVLMWMPLVVIGLMILRGITSYISSYCISWVSGKVVMTMRR
                     RLFGHMMGMPVSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIMM
                     FYYSWQLSIILIVLAPIVSIAIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGHKEVL
                     IFGGQEVETKRFDKVSNRMRLQGMKMVSASSISDPIIQLIASLALAFVLYAASFPSVM
                     DSLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFTILDSEQEKDEGKRV
                     IERATGDVEFRNVTFTYPGRDVPALRNINLKIPAGKTVALVGRSGSGKSTIASLITRF
                     YDIDEGEILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEQYSREQI
                     EEAARMAYAMDFINKMDNGLDTVIGENGVLLSGGQRQRIAIARALLRDSPILILDEAT
                     SALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGVIVERGTHNDLL
                     EHRGVYAQLHKMQFGQ"
     gene            complement(14231..16495)
                     /locus_tag="ACHKBL_002336"
     CDS             complement(14231..16495)
                     /locus_tag="ACHKBL_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309023.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC family protein"
                     /protein_id="HFO5819199.1"
                     /translation="MKITTVGVCIICGIFPLLILPQLPGTLTLASLTVFACVLVFIPF
                     KTVRYIALTLLFFVWGILAAKQILWAGETLTGATQDAIVEITATDGMTTHYGQITHLQ
                     GRRIFPAPGLVLYGEYLPQAVCAGQLWSMKLKVRAVHGQLNDGGFDSQRYAIAQHQPL
                     TGRFLQASVIEPNCSLRAQYLASLQTTLQPYMWNAVILGLGMGERLSVPKEIKNIMRD
                     TGTAHLMAISGLHIAFAALLAAGLIRGGQVFLPGRWIHWQMPLIGGICCAAFYAWLTG
                     MQPPALRTVVALAMWGMLKLSGRQWSGWDVWICCLAAILLMDPVAILSQSLWLSAAAV
                     AALIFWYQWFPCPEWQLPPVLRAVVSLIHLQLGITLLLMPVQIVIFHGISLTSFIANL
                     FAIPLVTFITVPLILAAMVAHLSGPFILEQGLWFLADRSLALLFWGLKSLPEGWINIA
                     ERWQWLTFSPWFLLVVWRLNAWRTLPAMCVAVGLLMCLPLWQKPRPDEWQVYMLDVGQ
                     GLAMVIARNGKAILYDTGLAWPEGDSGQQLIIPWLHWHNLEPEGVILSHEHLDHRGGL
                     DSILHTWPMLWIRSQLNWEHHQPCVRGEAWQWQGLRFSAHWPLQGSNDKGNNHSCVVK
                     IDDGTNSILLTGDIEAPAEQKMLSRYWQQMQATLLQVPHHGSNTSSSLPLIQRVNGKV
                     ALASASRYNAWRLPSSKVKHRYQQQGYKWLDTPHQGQITVNFSAQGWRISSLREQILP
                     RWYHQWFGVPVDNG"
     gene            complement(16702..16986)
                     /gene="ihfB"
                     /locus_tag="ACHKBL_002337"
     CDS             complement(16702..16986)
                     /gene="ihfB"
                     /locus_tag="ACHKBL_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006417 - regulation of translation
                     [Evidence IEA];
                     GO_process: GO:0075713 - establishment of integrated
                     proviral latency [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HFO5819200.1"
                     /translation="MTKSELIERLATQQSHIPAKTVEDAVKEMLEHMASTLAQGERIE
                     IRGFGSFSLHYRAPRTGRNPKTGDKVELEGKYVPHFKPGKELRDRANIYG"
     gene            complement(17146..18819)
                     /gene="rpsA"
                     /locus_tag="ACHKBL_002338"
     CDS             complement(17146..18819)
                     /gene="rpsA"
                     /locus_tag="ACHKBL_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HFO5819201.1"
                     /translation="MTESFAQLFEESLKEIETRPGSIVRGVVVAIDKDVVLVDAGLKS
                     ESAIPAEQFKNAQGELEIQVGDEVDVALDAVEDGFGETLLSREKAKRHEAWITLEKAY
                     EDAETVTGVINGKVKGGFTVELNGIRAFLPGSLVDVRPVRDTLHLEGKELEFKVIKLD
                     QKRNNVVVSRRAVIESENSAERDQLLENLQEGMEVKGIVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEIVNVGDEITVKVLKFDRERTRVSLGLKQLGEDPWVAIAKRYPE
                     GTKLTGRVTNLTDYGCFVEIEEGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLD
                     IDEERRRISLGLKQCKANPWQQFAETHNKGDRVEGKIKSITDFGIFIGLDGGIDGLVH
                     LSDISWNVAGEEAVREYKKGDEIAAVVLQVDAERERISLGVKQLAEDPFNNWVALNKK
                     GAIVTGKVTAVDAKGATVELADGVEGYLRASEASRDRVEDATLVLSVGDEVEAKFTGV
                     DRKNRAISLSVRAKDEADEKDAIATVNKQEDANFSNNAMAEAFKAAKGE"
     gene            complement(18930..19613)
                     /gene="cmk"
                     /locus_tag="ACHKBL_002339"
     CDS             complement(18930..19613)
                     /gene="cmk"
                     /locus_tag="ACHKBL_002339"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HFO5819202.1"
                     /translation="MTAIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLA
                     LAALHHHVDVASEDALVPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQ
                     VAAFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAHRRML
                     QLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVPAADALVLDSTTLSIEQVIEKALQ
                     YARQKLALA"
     gene            complement(19786..>20542)
                     /gene="ycaL"
                     /locus_tag="ACHKBL_002340"
     CDS             complement(19786..>20542)
                     /gene="ycaL"
                     /locus_tag="ACHKBL_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="metallopeptidase YcaL"
                     /protein_id="HFO5819203.1"
                     /translation="TKLLLAIAASAALLTGCQNTHGIDTNMAISSGLNAYKAATLSDA
                     DAKAIANQGCAEMDSGNQVASKSSKYGKRLAKIAKALGNNINGTPVNYKVYMTSDVNA
                     WAMANGCVRVYSGLMDMMNDNEIEGVLGHELGHVALGHSLAEMKASYAIVAARDAISA
                     TSGVASQLSRSQLGDIAEGAINAKYSRDKESEADDFSFDLLKKRGISTQGLVGSFEKL
                     ASLDGGRTQSMFDSHPPSTERAQHIRDRIASGK"
BASE COUNT         4901 a         5693 c         5026 g         4922 t
ORIGIN      
        1 aaaaggcggc attgctgccg ccttcatttt aactcgcttg gtaaggcgct tcgtcacttc
       61 ctggaagcgt ttcagggagt tgcggcgcaa atcctcgcag gccgacgacg tgaacgtgtt
      121 cggtattctg gaagacttta cgcaccagtt tataggtggt gcctttttcc ggactgatat
      181 tttccggcgc tgcgatgatg agctgcattt gcaaacgttc acacaattca aacagcgtgg
      241 cgatagaacg agcatccagt cgcgctgctt catcgaggaa cagcaggcgg caaggagaga
      301 tatctttacc gcgcaggcgg cgagattcat cttcccagct ttgtaccacc atcaccagaa
      361 tcgacatacc ggtaccaatc gcctcaccgg tcgacagagc gccagattct gcgcgcagcc
      421 agccatcaga accacggtta acctcaactt ccatttccag atagttgcgg taatccagca
      481 gttcttcgcc aatggtctgc ggcgtgcgct gccccatatc aatctgcgga ttaagacgct
      541 gatacaattt cgccagcgct tccgagaagg tcagacggtt gctgttaaac agatcctgat
      601 gctgctcgtg ctgttcagag agcacatcca gcagcatggc gtgcgtttca cgcacgttca
      661 cattcagacg tacgctgttt acctgaccga acgatacgtt ctgcaaaccc tggttgagca
      721 tacggatacg gttctgttcg cgctgaatgg ttttgcgaat aatgttcgcc acgctgcggg
      781 aactgatcgc cagtttctgt tcacgggagg ttaactcttc ggtcagacgg ctaagttcga
      841 tctccatctg ttcgatagct tccaccggat catcggtacg aataatatcc tgacgaatac
      901 gttcacgcag atgctgataa accgccacga agaactgaat tttacgttcc ggacgttttg
      961 gatcttccga catccgcagc acgtcgcgca gatgttcgtt atccgccacc gccagacgca
     1021 gcgcacctaa cgccttatcc gacatggaac gcaaatcatc agcggagaga taagccagct
     1081 cacgacggtg taagcggcgt tcaacgccgt tatctttcac catgcgcatg actgcacacc
     1141 agcccgcttt cgcggtcact acctgctcgc gcatctcaaa gtaatcacgc tccagcttgc
     1201 gcagtttgcg ggtcaggttg tccatctccg cttcgcagaa ggtaagcgct ttttccagtt
     1261 gattgcggcg tgaacggtta ttgctcagtt gcgcgtgcaa ctcgtcacgg cgaatacgcg
     1321 cccgctcttc tgccccgcta tcagcacgca cgccgatatc ctgcaattca cgttgcagat
     1381 cgttgagtag ctcttttttg gtgtcgtaag aacttttcag cgaagccagc acctggttgt
     1441 actgactcaa ctgcgctgcg tgtccgcgca acgcttcacg cgcgcgggta cgctccgctt
     1501 ccgcctgttc cagacgttca cgcagttttt cgttgagatc gctgttaccg ctcagcattt
     1561 ctgccgagtc agagtagcta aagtgcgcac gacgctgcac cacttccgtc agggcaaacg
     1621 cctgctgacg ggcatcgcgc tgcatctgct gagagtacgc gtaatcttct tttaactgtt
     1681 cgaactgttc cgggtcgctc tgcaataccg aaacgatcgg ttccagtttc gccagttgat
     1741 tgccaaactg ctgaacaaaa cgcgcggctt cctgggcttc atccagacgt tcgcggattt
     1801 catcgacgcg atccgccagg ctgtcatcag ccaacaggtt gagacgcggc agaatgcggt
     1861 tcagcgccgt aacgccctct ttcgcctgct caaattgaat acgctgctgc tggttatcat
     1921 tttcatgatt actcagcgcc cgctccagtt cgacgcgacg gctgttcagt tgacggattt
     1981 cggcttccgg atcagactca aacgccaccg ccagatgact acctataaag cggctgaacg
     2041 cctgatgcag acgctgggtt ttctgtacat caaaagagag cgtggcgaag cgttcggaaa
     2101 gcacttcacg ctcggcatgg aggctttcaa tacggctttc acgcgcagca cgaccaaaca
     2161 gcggaacttc cggaaaacgt gagtaacgcc actgacgatc ggcgattttc accactaccg
     2221 ctttttccag ctcatcaacg ctgaacacgc tgtcatcgaa tgactgcgga tccccttcga
     2281 tcagatagag atcttccggg caatcggtca agccttccag gtgttcggtt acctgtgaca
     2341 gatctggcac cacgatggcg tggcgtgacg ggccatacag tgccgagaag tacggcgcat
     2401 cttccagact cacgtcgtca taaatttcgg acagcagcac gccgccaaaa cgctccgcca
     2461 gcgcattcaa acgctggtct tcagaaccac ctggctggct taaacgttcg atctcttcat
     2521 cgacggcgtt tttgcgcgcg cctacttcat cgcgttcaac aatggcttcg cgctcacgct
     2581 ccagcaactg ttgcagatat tcagtgacgt cctggctgga ggtgaactct tcgccgcact
     2641 gttcgctcaa ctggttgaga ctgttttgcg ctgccagcca aaccggtgca cgctgcatca
     2701 aactctgaat gcgagactgc agctgttcct gttcctggcg cagtgccatc cgctcttctc
     2761 gggcgttaga gacgctatcg gaaagagagg caatgcgtgc ttccagctcc tgatgcagag
     2821 cttccagttc atcaatatca aaattcttgc cctgacgttt gcagaaatct gccagcagac
     2881 gctcagcttc ttgctgctca cgcagacgct gttccagttc gcttaagcgc atccgcaacg
     2941 gctgaacctg ctctgccagg tgacgctgat cgaccccttc gcgcaatagt tcgcgagcga
     3001 catcccacgc ctcgttacgc gccagcgggc cgttgattgc caccaccagc tgataagcct
     3061 gctcaaactg gctgtgcgcg gtttgcgcca tgctcatttt ctgctcaaga gagagcattt
     3121 tttcggtcgc ttccagctct ttcgcctgga aggtttccag ccattcggca gcgctgtcgg
     3181 cggttaagtc cggcagatgg cacagttctt tggcacgatt aagcgcagca atcgcctggt
     3241 tatactggat cgcgcgcgtt tgctggacgt ccagcgcctg ctggtagtca gcaagctggc
     3301 ttttcagctc gtccacttcc agttcggcag cttccgcacg agcctcgttc tcttcctggc
     3361 gttcgatggc ttctgccacc acttcgtttt gctcttccag acggatctgc agctcatcga
     3421 gatccgcttc gtagcgttcg atcttctcct gctgacgcag tgcggtttgc accaggttca
     3481 ggtgatcgct ggccgcctga taatccgctt ccagatcgcc ttcggcaccg ttgtgctcag
     3541 ccagctctcg cgccatgtcg acgtgtttgt actgctcagc cgccagttgc tgacgcgaag
     3601 tatgtagctc gcgacgaaac tccagggcct tatcgagatg aacacggcgc tcgttggcgt
     3661 gacgcatgta gtccgccgcc acgtagttgg tggcttcgct gatcagatgc ttaaacaggt
     3721 cacggtctga ctgggtgaca cgaatcgctt ccagcgtcat gcggttttca cgcagcgccg
     3781 cttccatgtc ctggaacgct ttccgcaccc cactgttttc cggcaacagg tagtcgcgca
     3841 gagaacgagt aatggcgctg gagatcccgc catacagcga ggcttcgatc agacgataga
     3901 atttactacg gtcagacgct gagcgcagac gacgcgcgat gatgcccaga tcgaacatca
     3961 gcgagtggta atcggtaatg gagttgaact gtttaaactg cacgccttcc atcgcctcga
     4021 gcttgtcttt cagctcgtta agcggcagca cgcgcgcctg gcgttcgttc agagtttcgg
     4081 tcaccagttg tgtcggctgc accgacatcg gcaggccctg aatggcaaat ggtttgatat
     4141 ccactttacg atcgcgtccg gcaacctgtt gcagacgcac accgaccact acacgctggt
     4201 ggcgcgagtt aatggtgtcg agcatcgaat aacagacacc cgctttcagc ttaccgtgca
     4261 gacctttatc gcgcgaaccg ctggtggccc cggcttccgt agtgttacgg aaatgcagca
     4321 gggtcaggtc ggggataagc gccgtaacga acgctgccat ggtggtggat ttacccgccc
     4381 cgttaccgcc agaaagcgtc gtgaccagct cgtcaaggtc aaaagttcgg gcaaaaaagc
     4441 cgttccagtt aatcagcgtc agtgagcgaa atttaccgcg ttcaatcatt attcttcctc
     4501 tccgctatct ggctgactct cttcggtttc atcgttgagt tgcagatgat tttcaatcgg
     4561 cattgcttcg ccatcacgaa tcaggcgacg ctgtgcttca cggggatcgt cgccagcacg
     4621 cacatcggct ccgaagcgga acaccgattc ggtaatgcgg aacttgctgc tgtcgtggcc
     4681 cataaaccac accataccca aacgacgcag acggttgagc gaagaacgta ctttctcctg
     4741 caacttctgc cggtcaacgt ctgaaccggt tgaacggttg ttcaccagtt tcagcagttt
     4801 cgcttcatcg gccagggtga gcagttcgtc gtacagttcc tgctgggtga aaatcccctc
     4861 attcgccagc cgttccgggc tgagatagag ataacagagg attttcccga ccatcatatc
     4921 cagttccgac aagacggaac gagggatcag cgtggtggaa cgtgggcgta gatagaagaa
     4981 cccttccggt gcacgaataa gctcaacgtt ataacgcgcg taaaactctt ccagatattc
     5041 ctgaaaatcc atcaggaatg catgattatc cagttcgtcg aggccaatat gacggcctga
     5101 acgtaaggcg ctatccagcg ccggaaataa cggattcgcc agcgcctgcg ccagcttaac
     5161 cggcatcact tgttcaatat ttgtcgatga catgcgcctg taccttggct ccgtaatcat
     5221 taatcggctg ccatttcgct ggcagtccgg tgaaatctgc ttgcgctacg ccaagacgta
     5281 ccgcctgatc aataacaatg cgcgcaacgt caaagtgacg tgcacgcgga tactgtgaca
     5341 gatattcgcg taccaccaga ccaagatcca acggcacttg tctggttttg tacacggcaa
     5401 gttgttcttc gatgatcgcc gccagctgtt cgcggatctc gttaaactct tcgtactcca
     5461 gatcctcagg aagttcccca gtcacttctt catcgcgcag taccatctct tcgtcacgca
     5521 tatccagcag acgatcggca ttggcataag ttagcgccca cggctcatca aaataggttt
     5581 gtaccgactg acgtaaccgc tgagcaaaga cgcggttttt atccatatcg atcgcagtac
     5641 ggataaattt gtgtacgtgg cggtcgtagc caatccacaa gtctatggat tgctggcccc
     5701 aactgataat acggtcgagt ttgctctgca gatcgaacac cagacgatcg acaaaatgca
     5761 ggtcgtcatg ggtcatcgtc gcatcctgaa tgcgcaacag attagcctgc aatttatcgc
     5821 ctgccgcttc cagcgtatcc tgcaattcac gcagcgttcc ggaagtttct gaaagcaaca
     5881 attcacagct ggaaatcgcc gcccgccagt ctttgttcaa caactgggcg atatcatcct
     5941 tcacctgctg ctgctgttcg tccatcagac gttgcgtcag gtcgatactg tcgaaaattt
     6001 ctgctaccga atatttcagt ggcgcataaa cattacggtg ccagtgaaat tcatcaccgc
     6061 cctcttcggc ggcatctgcc gcgcgtttga gctcacccgc cacaatcgac aactgcatag
     6121 aaaggcgcag cgtagaaaac tcgcgctgac ggatgtaata gtcagtaatg ccgatgccga
     6181 gcggcgtcag acggtaaatt gcgttccctt ccgcctgctc gctggtaaag cggttcagca
     6241 gacgttgacg caccatgtcg ttgatcgcgt tgttggcgcg cacgccgatg gtttcgctgg
     6301 tttgctcaaa cgcatcactc acatggcgga atgcatccac cagctcgcct tcactcatct
     6361 caccatccag acgctcgccg ttcagcgtgg caaccgccag cagaaaagag agtcggtcta
     6421 ccggcagcga gatggagaag tcattttttc tggcccaggc aaccagttcg gggactgtct
     6481 gggaaaattc actcatactt tatccttgct ctgcggtttg cgcgcggtga catgaatata
     6541 acgccccagg gtaatatacg gttcctgacg gcaataacgc gtttctaatt caagtaatgc
     6601 ttcatagcag tcgcgctgct ggtgcttctc gcgcagataa tcatgaaaca cgcgaacgcc
     6661 tgtcttaccc ataatttgcc aaccagcttc ttccagccac agataaacct gcgccgggtc
     6721 gcgtggataa tctggcgaaa gcgtccgttt tttctttttc ggcattcccg cctgcacgta
     6781 atcaaaattc ccggcgacca tgttatgcat cagcaagccg tgcgcattgt agaacattaa
     6841 cgacaacaca ccgcctggac gcaacactga ccacagggtc tgtaatacgc tgcggggatc
     6901 ggccacccac tcgagcaccg catggaacaa tatcagatca acgggcgttt ccaaatgtga
     6961 agcaacatcc tgagcggcgc aatgtataaa ttgcatgttg tcgctcacac cttttgcttc
     7021 tgccgcctgt tttgcgcggt cgatcatctg cgcagaaaga tcgcataaaa tgacctgatg
     7081 cccgcgctca gccattttga ttgcggtctg cccttctcca ccgccagcat ccagcacacg
     7141 cagtttttgc gggcccattt ccgccagcac gcgatcgaga tcctgccaca gaatagcctg
     7201 tcgaagctgc cctttggtgg tgccgtaaat gttacgggaa aacttttccg caatatcatc
     7261 aaaattgcga tcctgcattg ctttctccac cggttggcta agacgttttc tgataaagac
     7321 gctattttgt cacagccacg gcgagaatga acccgtctgg tgtaatatcg ctggcgatcc
     7381 cttgctatat ggttaaaaaa ggaaccagaa aggatgcttt ttacactgaa aaaagtgatt
     7441 ggcaatatgt tgctgccact tccgctaatg ttgttgatta ttggcgctgg cctggcgctg
     7501 ctgtggttta gccgttttca aaaaaccggg aagattttta ttagcattgg gtggctggcg
     7561 ctcttgttat tgagtttgca accggtagcc gaccgcctgc tgcgtcccat cgaaagcacc
     7621 tatccgacat ggaataattc gcaaaaagtg gactatatcg tggtgctcgg aggtggttat
     7681 acctggaacc cgcagtgggc accaagctcg aatttaatca ataacagtct gccccgcctg
     7741 aatgaaggta ttcgcttatg gcgcgaaaat ccgggatcaa aactgatctt cacgggaggc
     7801 gtagcaaaaa ccaatacggt gagtacagcc gaagtaggtg cccgagttgc gcaatcgctg
     7861 ggcgtgccgc gcgagcaaat tatcactctg gatttgccaa aagataccga agaagaagct
     7921 gcggcagtga aacaggcgat tggtgacgcc cctttcctgc tggtgacatc cgcgtctcat
     7981 ttgccgcggg cgatgatctt ttttcagcag gaagggttaa atccgctgcc agccccggcg
     8041 aatcagttgg ccatcgactc gccgcttaac ccgtgggaac gggcaatccc ttctccggtc
     8101 tggttgatgc atagcgatcg tgttggctat gaaacgcttg gccgtatctg gcagtggcta
     8161 aaaagttcgt caggcgagcc acggcaagag tgattttgat gccagatcga agttgcgccg
     8221 actaaatcgt ccggtattaa ccagttgcgc aacttcatcc cataacacat acaaccaacg
     8281 ccgccagatg aacgactccg ccactggcgc gcgttgcagg taactccaga gcaaatcttc
     8341 cgccagagaa gtatccatta gtcggaacag ttcgtattcc cgcggtgccc aaagcattag
     8401 tcccggcccg accatcgcca gtaactgatc gctgcgcgaa tctttcagca tgctgcgtaa
     8461 acagaagtta ccgtgaatca gcacacaatt gtcgttaaag ccttcaaata atgccgggag
     8521 acattcgcga gtacgaaaca ggatccgctt atcctgcatc gttagaccgg tgttattgaa
     8581 ctgattgagc gtggtccata gcacttcaac atgttgccgg taccatgagg gccagaaatt
     8641 ttcctgagta ttgtcgaccg cgccgacgca accgcgactg tcctgacggt gccaggccag
     8701 taaggcttca acgatttgat ctttgagttg ttcccagcgt tctggtgttc gggctggtgc
     8761 ctccaccgaa acaccacgca tccgctccag tagcaggaca tcggggccgg ggtgttcttc
     8821 atgcgtcatc acaccataaa tagtcggcat acggacagtc ccacttcttg ccagcatggt
     8881 ggttttccat gccagttgtc gggcttttcc gggcgtacta aagcttcttg ccattaacgg
     8941 cattgggttt ccctggctgt catacaaagc ccacaacgcc gtatccgctt tttcattgac
     9001 gcactcaata cgactgagtt tttcgcccag taaatggctt aattcggcac gcagctgttc
     9061 cattcagatt accctcataa aagctactgc tttcataatg agcgccaata taaaagatgt
     9121 cacctgaatg agatcaattg ttgaaaagaa aaattgagga aaatcgtaac ggtacgacac
     9181 tcctcccaaa attggctgga gtgtcgtgaa gtgaaattag cgcatttcag cgcgaacgcg
     9241 ctcgagatct tcaggggtat ccacacctgt gccaggaact tcctgggcga cagcaacatg
     9301 gattttttcg ccgtaccaca gaacacgaag ctgctctaac atttcgatgt gttctaacgg
     9361 acttggctgc cagttgacgt aacgacggat aaagcctgca cggtagccat aaataccaag
     9421 atgacgcagg aagttatcgc caacggtttc aaggcctttt gcaaaacgat cacgatccca
     9481 aggaatggtg gcgcgagaga agtacagtgc atacccttca gcgtcgagga ccactttcac
     9541 cgcattcggg ttaaacgctt cctccgcatt gtggattggc accgccagag tcgccatacc
     9601 cacctgacgc tgagcgaggt tatcagcaac ctgacgaatg attgtcgcag ggatcatcgg
     9661 ttcatcaccc tgcacattaa cgatcacagt gtcgtcgctg aatgcgcatt tttcgacaac
     9721 ttccgccaat cgttctgtac ctgactgatg atcggcgcgc gtcatacata cttcgccgcc
     9781 agcggcttca acagcgcggg caacatcctc atgatcggtt gccacgatga tgcgctcggc
     9841 acctgattcg cgcgcgcgtt caagaacatg aacaatcatg ggtttgccgt taatatcaac
     9901 caatggttta ccgggcagac gcgtggacgc gtaacgcgcg ggaataatga ccacaaaact
     9961 catgatttac tctcatcagc agtcagcacg cgggcttccg tttccagcaa cactggaatg
    10021 ccatcgcgca gcgggaaagc caggttgtcc agtttgcaaa taagctcttg tttttcctgg
    10081 ttataccaaa gttttccgtt gcaaaccggg caggcaatga tttcaagcag acgatgatcc
    10141 atagttcctc cagatggatc gggttatgaa tgcataaatc ttatcataat catttatgcg
    10201 acggggtcta tttcccagcc ggcgcggcaa tcagtaaaga ggccttgcgg gcagcgtcct
    10261 aatgtcaccc gcgttgcctg ctgccagtta gcgaaatcag taatcgcctg acgtaaccct
    10321 ttttgcagcg ttgtcgttgg tttaatgcct tcttgtaacc acagagagat aacttcaagg
    10381 atgcctgtct ggcgatgcat tttggcatcc attcgcccaa ctaattgccc acgatgtaat
    10441 aacggcagaa caaaatagcc atactggcgt ttcggcgctg gggtatagca ctccagccgg
    10501 tagctaaaat caaaaagctg ctctgcgcgt ttgcgatccc agacaacagg atcaaaaggc
    10561 gaaagtaccg cgctgtgcgt tgcagtgagc tttccggcca gcgcacgctc gagtagcggc
    10621 agcaaatcag catgcagcca aagattgccc aatttttcaa catgcacagc aatgatttgc
    10681 tgctgttcag ccctcgcttc acgccacgcc gccagtgccg gacgttttag ccggtagtaa
    10741 tctgccagcc actgttcgcg aaatataccc aggctacgcg cactgttatc cagcatgatg
    10801 atttctgctt ctgcttgcga aacgagatcg cgctcatcat tccactcagg catgacacgg
    10861 tgagttaaat cataaacgcg ctggaagttg cgccgttcaa tcaccatcac ctttccggca
    10921 gtaaataaac cttccagatg tcgtttatgc ggcttccact cccaccagcc gctcgcccct
    10981 ttacgaggat gctcaaaatc ggctgaacgt actggcccct tatcatgaat atgctgaatt
    11041 aactgtgcaa tttccgccgc atgttcctgc atccaggcgt ctttatattt ccagcccatt
    11101 ttttcaggtg ccagcatgcg gtggcgaata agacgaaaat cgctacgcgg cataaagcag
    11161 gcttcatgcg cccagtattc cattaattcg ccacgcgcca gagactcatc cagccactgg
    11221 gcaggataat ttcccagacg actgaaaagc accagatatg gactacgggc aacaatattg
    11281 atggtatcga tttgcagcaa ggacatgcgg gagatcgttg ccggaatatc ccccaacgac
    11341 gctcgacggc ggggtttgtt taacaggcct tgcgcggcga ggtgaagatt acgcgcatca
    11401 gcaagagaga ggtgcggcag cgacattcat gactccatca atcgaacgct gccgcggcgt
    11461 aactagttgc cagaagccag cgaggttagt tgcgcaagca gtttcgctgg ttcatcacct
    11521 gaaagctgtg cgtcaacagg caaataccac caatttcctt cggcaaaggc ccggcatttc
    11581 accgcatctt tttcagtcat taccagcgtt tgcccggcgt ttaccaacgc gctgacatcc
    11641 gcatggttca aagactgatg atcggccagc ggtacacatt tttccggttg tacgccgcac
    11701 atcttcagcg tggcaaaaaa gcgcggcgga tgtccaatcc ccgccatcgc cactacatgt
    11761 tcaagctgag caacgtcaca acgcgtaccg gtacgtaaat tcaccgcctg acccggcagc
    11821 agatgcatgg ggatttcacc gctgcggggg acaccgccgt tgacgattac cgcatcaacc
    11881 gactttaagc gccccgctcg ctcacgcatt ggccccgccg gcaaccacca gccattgcca
    11941 aagcgacgca caccatcaat aacgacaatt tccacatcac gcgccagacg ataatgctgt
    12001 aaaccatcgt cggttacgat gatctgcaca tcagggtgtt gcgccagaat ggcttttacc
    12061 gcgtcagaac gcacgggaga aaccgcaaca ggcgcgccag tgcgttgata aatcaacaca
    12121 ggttcatcac ccgcctgtgc tgtggtggta tctgccgaca ataacagcgg ataagattca
    12181 gccttaccac catatccccg cgatacaacc cccacgcgaa taccgcgctg ttgcagctgt
    12241 tccaccagcc agacaacgac cggggttttt ccgttgccgc ctgcggtgag attaccaacc
    12301 acgacaaccg gtacgggggc acgccaggcg cgcttcagtt ttagtttata gcaaagacgg
    12361 atcgcgccac tcaccaggcc atacaaccag gagagtggca gcaatagccg ccataaaggg
    12421 gattcaccag accagatttt ttcgatcatt ggccaaactg cattttgtga agttgcgcgt
    12481 aaacgccacg gtgctcaagc aaatcgttat gcgtaccgcg ttccacaatg acaccatcct
    12541 cgacaaccac gatttcgtct gccttttcaa tggtagacaa gcggtgggca atcaccagag
    12601 aggtacggtt tttctgcaac tcatccagtg ccgcctgaat cgcacgttcg gattcggtat
    12661 ccaacgccga ggtagcttcg tccagaatca gtatcgggct atcacgcaac aaggctcgag
    12721 cgatagcaat acgctgacgc tgaccgccag agagcagcac gccgttttca ccaatcactg
    12781 tatcgagacc gttatccatc ttattgatga agtccatggc gtaggccata cgcgccgctt
    12841 cttcaatttg ctcacggctg tactgttcag tccgtgcgta agcaatgttg ttagcaaccg
    12901 tatcgttaaa cagatggaca ttctgcgaca ccagagcaac ctggttacgt aacgacgcca
    12961 gggtatactc gcgcagatcg tgaccatcca tcaggatttc gccttcatca atatcgtaaa
    13021 aacgcgtgat caggctggcg atggttgatt tacctgaacc agagcgtcca accagagcaa
    13081 ccgtcttccc tgccggaatt ttcaggttga tgttacgcaa tgcgggcaca tcacgtcccg
    13141 gataagtaaa ggtgacattg cggaattcca cgtcgccagt cgcacgctcg atcacgcgct
    13201 taccttcgtc tttctcctgc tcactgtcca gaatggtaaa cagcgtctga caagccgcca
    13261 taccgcgctg gaactgggcg ttaacgttgg tcagcgattt cagcggacgc ataagtgcaa
    13321 tcattgaaga gaaaacaacg gtaatcgtac cggcagtcag gctatccatg acacttggga
    13381 agcttgccgc atacagaaca aacgccagcg ccaaagaggc gatcagctga atgatcggat
    13441 cagagatgga agaggctgaa accattttca tcccctgaag acgcattcgg ttgctgactt
    13501 tatcgaagcg tttcgtttcc acttcctgac caccgaaaat caatacttct ttatggcctt
    13561 tcagcatttg ttcggcgctg gtggtcacct gccccatggt gttctgcatg tttttactga
    13621 tgttgcgaaa acgcttcgat acaacgcgaa tcgcaatcga aacaatcggt gccagcacaa
    13681 tcaaaatgat cgacagttgc caactgtaat agaacatcat gatgaacagg ccgatgatcg
    13741 acgcaccttc acgtacaaca gtaatcagtg cgccggaaga agaagaagcg acctgttcgg
    13801 aatcgtaggt gatacgtgac aacagcgtac ccgttgactg tttgtcaaag aatgaaaccg
    13861 gcattcccat catgtgacca aacaggcggc gacgcatagt cattaccacc tttcctgata
    13921 cccaggagat acagtagctg gagatatagc tggtgatacc acgtaaaatc atcagcccga
    13981 tcaccaccag cggcatccac atcagcacgg agcgatctgt tttaccaaag ccatcatcaa
    14041 gaagtggctt aaggagcgat aacatgaagg tatcgctggc tgcgttgagg attaacgcta
    14101 cgcccgccac gatcagaccc gctttaaaag gcgcaatggt tggccacagt cggcggaatg
    14161 tctgccacgt agagagatct ttgtcgttat gcattcaaaa aaccagcatt tgttgaaata
    14221 gccgcatatt ctacccgtta tccactggca cgccaaacca ctgatgatac caacgaggta
    14281 aaatttgctc tctgaggctg ctaatccgcc agccttgcgc tgaaaaattg accgttattt
    14341 gaccctgatg tggagtatca agccacttat aaccctgttg ttgatagcga tgtttaactt
    14401 tgcttgaggg cagtcgccat gcgttatagc gcgatgccga tgcgagtgcc acttttccat
    14461 tcactcgctg aattaatggc aacgatgatg aggtattact gccatggtga ggtacctgaa
    14521 gtaatgttgc ctgcatttgc tgccagtaac ggcttagcat cttttgttca gcgggggctt
    14581 caatatcacc ggttagaaga atgctattcg tcccgtcatc aatcttaacc acacaggaat
    14641 ggttatttcc tttatcgttg ctaccttgta aaggccagtg cgcgctgaaa cgcaatcctt
    14701 gccattgcca cgcttcgcca cgcacacagg gctgatgatg ttcccagttt aactgacttc
    14761 ggatccataa catcggccat gtgtgcaata ttgagtccag ccctccccgg tgatccagat
    14821 gttcatgact caaaataacg ccttccggtt caagattatg ccagtggagc caggggataa
    14881 tcagttgttg cccactatcc ccttcgggcc aggccagtcc cgtgtcatag agaatcgctt
    14941 tgccgtttcg cgcaatgacc attgccagcc cttgcccgac atcaagcatg tacacttgcc
    15001 actcgtcagg tcgaggtttt tgccacagcg gcaagcacat cagcaagcct acagccacac
    15061 acattgctgg caacgttcgc caggcgttta atcgccatac caccagtaag aaccatgggg
    15121 aaaatgttag ccattgccaa cgttcagcaa tgttgatcca cccttccggc aaactcttta
    15181 acccccagaa aagtaaagcc aaagaccggt cggcaagaaa ccataaccct tgttccagaa
    15241 taaacggccc gcttaaatgc gcaaccatcg cggcgaggat caacggaacc gtgataaatg
    15301 tcaccagggg aattgcaaat agatttgcaa taaacgaggt cagactaatg ccatgaaata
    15361 tgacgatttg cacgggcata agcaggagtg tgattcccag ttgtaaatga atgagggaaa
    15421 caactgcacg caataccggc ggcagttgcc actccggaca gggaaaccac tggtaccaaa
    15481 aaatcagtgc cgcgaccgca gcggcagaga gccataaact ttgcgagaga atggcaacag
    15541 gatccatcag caaaattgcc gccagacaac atatccatac atcccagcca ctccattgtc
    15601 gcccacttaa cttaagcatt ccccacatgg caagcgccac cacggtacgc aatgcaggag
    15661 gttgcatccc agtcagccag gcataaaaag cagcacagca gattccgcca attaatggca
    15721 tttgccagtg gatccagcgc ccaggcagaa aaacttgccc accgcgaatg agtccggcag
    15781 ccagcaacgc cgcaaaagcg atatgcaatc ccgatatcgc cattaaatgc gccgttccag
    15841 tatcgcgcat gatatttttg atttctttgg gaacggataa ccgttccccc atacctaaac
    15901 caagaataac cgcgttccac atatagggtt gcagcgttgt ttgtagtgac gccagatact
    15961 gtgcacgcag gctacaattc ggttcaatga cacttgcctg cagaaagcgg ccggtgagcg
    16021 gctgatgctg ggcaatggca taacgctggc tatcaaagcc gccatcatta agttgaccat
    16081 gaactgcacg aactttgagt ttcattgacc acagttgtcc agcacaaacc gcttgcggaa
    16141 gatattcgcc atacagcacg aggcctggcg cagggaatat acgtcgacct tgtagatgag
    16201 taatttgacc gtaatgagtg gtcataccgt cagtagcagt gatctcaaca attgcatcct
    16261 gcgtcgcgcc agttaaggtt tctcctgccc acaaaatttg ctttgctgct aatatgcccc
    16321 aaacgaaaaa cagcaacgtc agcgcgatat aacggacagt tttaaaaggg ataaatacca
    16381 gtacgcaggc gaagacggtc agagacgcaa gggttaatgt cccaggcaat tggggcaaaa
    16441 tcagcaacgg aaaaattccg caaattatgc atacgccgac tgtcgttatt ttcatgcacc
    16501 cctccctgtt tatggaaagt gtgcaacttt gttgagcggt tgtctggtgg gagttttgcg
    16561 ttccccgagg cacgttcaga agaatttaac gagagggtga aaactgttca aaactctgcg
    16621 aggctgcttc cagttccaga ttagagaaat gaaaaaagca cccgacaggt gcttttctcg
    16681 cgttcaagtt tgagtaaaaa cttaaccgta aatattggcg cgatcgcgca gttctttacc
    16741 tggtttaaag tgaggaacgt attttccttc cagttctact ttatcgccag tcttcggatt
    16801 acgtccggta cgtggtgcgc ggtagtgcaa agagaaactg ccgaaaccgc ggatttcaat
    16861 acgctcgccc tgcgcaagag tcgaggccat atgctccagc atctctttta ctgcatcttc
    16921 aaccgtcttg gcgggaatgt gcgattgctg ggtggcaagt ctttctatca attctgactt
    16981 ggtcatgatt cctccggttc cttaaaggca aatttagcgg ctgctgttgc tgcattgatt
    17041 aagggcggcc gtagccgccc ttagtgcttg attacaggac gaaacctgca atctgtcaag
    17101 taaactcaac aaacttcgga ataaaaatcc cgaagagtca gagaattact cgcctttagc
    17161 tgctttgaaa gcttcagcca ttgcgttgtt ggagaagttt gcatcttcct gtttgttaac
    17221 agttgcgatt gcatctttct cgtcagcttc gtctttcgca cgaacagaca ggctgattgc
    17281 gcggttttta cgatcaacgc cggtgaattt agcttcaact tcgtcgccaa cgctcagaac
    17341 cagggtagcg tcttcaacgc ggtcacggga tgcttcagaa gcacgcaggt aaccttcaac
    17401 gccgtcagcc agttctacgg ttgcgccttt agcgtcaact gcagttactt taccggttac
    17461 gatagcgcct ttcttgttca gagcaaccca gttgttgaac ggatcttctg cgagctgttt
    17521 aacgcccagg gagatacgtt cacgttctgc gtcaacctgc agaacaactg cagcgatttc
    17581 gtcgcctttt ttgtattcac gaactgcttc ttcgcctgca acgttccagg agatgtcaga
    17641 caggtgaacc aggccgtcga tgccgccgtc caggccgatg aagataccga agtcagtgat
    17701 agacttgatt ttaccttcaa cacggtcgcc cttgttgtgg gtttccgcga actgctgcca
    17761 cgggttagct ttgcactgtt tcagacccag ggagatacga cgacgttctt cgtcgatatc
    17821 cagaaccata acttccacta catcgccaac gttaacaact ttggacgggt ggatgttttt
    17881 gttggtccaa tccatttcgg aaacgtgtac caggccttca acgccttctt cgatttcaac
    17941 gaagcagccg tagtcggtca ggttggtcac gcgaccagtc agtttggtac cttccggata
    18001 acgtttagcg atagctaccc acggatcttc gcccagctgt ttcaggccca gggatacacg
    18061 ggtacgttcg cggtcgaact tcagcacttt aacagtgatt tcgtcgccca cgttgacgat
    18121 ttcgctcgga tgcttaacgc gtttccaggc catgtcagtg atgtgcagca ggccgtcaac
    18181 gccgcccaga tcaacgaatg caccgtagtc agtgaggttc ttaacgatac ctttaacttc
    18241 catgccttcc tgcaggtttt ccagcagctg atcgcgctct gcgctgtttt cggattcgat
    18301 aacggcacga cgagaaacaa caacgttgtt gcgcttctga tccagcttga ttactttgaa
    18361 ttcaagctct ttgccttcca ggtgcagagt gtcacgcacc ggacgaacgt ctaccagaga
    18421 acctggcagg aacgcacgaa taccgttcag ctcaacagtg aagccgccct taactttgcc
    18481 gttgataaca ccggtaacag tttcagcatc ttcgtaagct ttttccagcg tgatccaggc
    18541 ttcgtgacgt ttagctttct cacgggacag cagagtttca ccgaagccgt cttctactgc
    18601 gtccagagca acgtcaactt cgtcacctac ctggatttcc agctcgccct gggcgttttt
    18661 gaactgctca gccgggatgg cggactcaga tttcagacca gcgtcaacca gtactacgtc
    18721 tttgtcgata gcaacaacaa cgccacgaac gatagaaccc gggcgggttt cgatttcttt
    18781 taaggactct tcaaagagtt gagcaaaaga ttcagtcatg tttaatcttc aggtttatat
    18841 ttaacgtcca cctggctcca tgccggatgg ggttgtttaa catacccgct gtcattccat
    18901 tgcaacgggg gtactgcaaa ttcggtcgct tatgcgagag ccaatttctg gcgcgcgtat
    18961 tgtagcgctt tttcaatcac ttgctcaatg cttaaggtgg tggaatccaa cactaaagca
    19021 tcggctgccg gaaccagtgg cgctaccgct cggttacgat cgcggtcgtc gcgttctttg
    19081 atctcagcca aaaggcgctc aaagttaaca ctaaagccct tctcctgcaa ctgtagcatg
    19141 cggcgatgcg cacgttcttc cgaggaggcg tcaaggaaaa ttttcactgg cgcatcaggg
    19201 aataccaccg ttcccatgtc acggccatcg gcaatcaggc ctggtaattc gcgaaacgcg
    19261 cgttggcgac gcaataatgc ttcacgaacg cgtgggaatg ccgcgacttg tgaagctgca
    19321 ttcgccactt cctgagtacg aatttcgcca ctgacatctt ccccttcgag gatcacttcc
    19381 agattgccat tggtcgacac aaaacgtaca tccagatggg atgccagcgg caccagcgca
    19441 tcttccgatg caacatcaac atggtgatgt aatgccgcca gtgccagtac gcgataaatt
    19501 gcacccgagt ccagcagatg ccattgcaac gcttccgcca tagccttaca caaggtgcct
    19561 ttccctgcac cgcttgggcc atcaatggta ataaccgggg caattgccgt catctttatc
    19621 tccttaaaac aggcgtaccg ttaacataac cgcgcgcatt atacgcgccg acgccacaaa
    19681 tcgttaacgt tgtgtgcaaa aaccagtttt gtctgtattc agatggggat ttattgcgca
    19741 attatagcgg gctggcagaa gaccagcccg aaagatgaca atgatttact taccagaggc
    19801 gatacgatca cggatgtgtt gcgcacgctc tgttgatggt gggtgagaat caaacatgga
    19861 ctgggtacga ccgccatcca ggctggccaa tttttcaaag ctgccaacca gcccctgggt
    19921 gctgatgcca cgtttcttca acagatcaaa ggagaaatca tctgcttcgg actctttatc
    19981 gcgggagtat ttagcattga tcgcgccttc tgcgatatca cctaattgtg agcgagaaag
    20041 ctgggaagcc acaccgctgg tagcggaaat cgcatcacgt gcggcaacga tcgcataaga
    20101 agctttcatt tcagccagcg agtgacccaa cgcgacgtgg cccagttcat gacccagaac
    20161 gccttcaatt tcgttgtcgt tcatcatgtc catcaggcca ctgtagacac gaacacaacc
    20221 gttcgccatc gcccatgcgt tgacgtcgct ggtcatataa accttatagt tgaccggcgt
    20281 gccattaata ttgttaccca atgctttggc gatttttgcc agacgtttcc cgtacttgct
    20341 ggatttgctt gcgacttgat tgccgctgtc catttcggca cagccctgat tggcaatcgc
    20401 ttttgcatca gcatcgctta acgttgctgc tttataggca tttaaacctg agctgatagc
    20461 catattggta tcaataccgt gggtattttg acaccctgtc agtaatgctg cagaggccgc
    20521 aatcgccagc agtaatttag ta
//
LOCUS       DAZFLD010000075        20537 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.075, whole genome shotgun sequence.
ACCESSION   DAZFLD010000075 DAZFLD010000000
VERSION     DAZFLD010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20537
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.075"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            218..370
                     /gene="hokA"
                     /locus_tag="ACHKBL_002341"
     CDS             218..370
                     /gene="hokA"
                     /locus_tag="ACHKBL_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012540334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokA"
                     /protein_id="HFO5819204.1"
                     /translation="MPQKYRLLSLIVICFTLLFFTWMVRDSLCELHIKQGSYELAAFL
                     ACNLKE"
     gene            complement(558..770)
                     /gene="cspA"
                     /locus_tag="ACHKBL_002342"
     CDS             complement(558..770)
                     /gene="cspA"
                     /locus_tag="ACHKBL_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone/antiterminator CspA"
                     /protein_id="HFO5819205.1"
                     /translation="MSGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNDGYKS
                     LDEGQKVSFTIESGAKGPAAGNVTSL"
     gene            complement(1051..1341)
                     /locus_tag="ACHKBL_002343"
     CDS             complement(1051..1341)
                     /locus_tag="ACHKBL_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator"
                     /protein_id="HFO5819206.1"
                     /translation="MEYKDPMHELLSSLEQIVFKDETQKITLTHRTTSCTEIEQLRKG
                     TGLKIDDFARVLGVSVAMVKEWESRRVKPSSAELKLMRLIQANPALSKQLME"
     gene            1775..2485
                     /locus_tag="ACHKBL_002344"
     CDS             1775..2485
                     /locus_tag="ACHKBL_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3053 domain-containing protein"
                     /protein_id="HFO5819207.1"
                     /translation="MATGKSCSRWFAPLAALLMVVSLSGCFDKEGDQRKAFIDFLQNT
                     VMRSGERLPTLTADQKKQFGPFVSDYAILYGYSQQVNQAMDSGLRPVVDSVNAIRVPQ
                     DYVTQSGPLREMNGSLGVLAQQLQNAKLQADAAHSALKQSDDLKPVFDQAFTKVVTTP
                     ADALQPLIPAAQTFTQQLVMVGDYIAQQGTQVSFVANGIQFPTSQQASEYNKLIAPLP
                     AQHQAFNQAWTTAVTATQ"
     gene            complement(2535..3509)
                     /gene="ghrB"
                     /locus_tag="ACHKBL_002345"
     CDS             complement(2535..3509)
                     /gene="ghrB"
                     /locus_tag="ACHKBL_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrB"
                     /protein_id="HFO5819208.1"
                     /translation="MKPSVILYKALPDDLLQRLQEHFTVHQVANLSPQTIEQNAAILA
                     EAEGLLGSNENVDAALLEKMPKLRATSTISVGYDNFDVDALTARKILLMHTPTVLTET
                     VADTLMALVLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
                     AQRAHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETHHLF
                     GAEQFAKMKSSAIFINAGRGPVVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLS
                     MANVVAVPHIGSATHETRYGMAACAVDNLIDALQGKVEKNCVNPHVAD"
     gene            complement(3613..4272)
                     /locus_tag="ACHKBL_002346"
     CDS             complement(3613..4272)
                     /locus_tag="ACHKBL_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family lipoprotein"
                     /protein_id="HFO5819209.1"
                     /translation="MKKRVYLIAAVVSGALAVSGCTTNPYTGEREAGKSAIGAGLGSL
                     VGAGIGALSSSKKDRGKGALIGAAAGAALGGGVGYYMDVQEAKLRDKMRGTGVSVTRS
                     GDNIILNMPNNVTFDSSSATLKPAGANTLTGVAMVLKEYPKTAVNVIGYTDSTGGHDL
                     NMRLSQQRADSVASALITQGVDASRIRTQGLGPANPIASNSTAEGKAQNRRVEITLSP
                     L"
     gene            4425..6758
                     /gene="bisC"
                     /locus_tag="ACHKBL_002347"
     CDS             4425..6758
                     /gene="bisC"
                     /locus_tag="ACHKBL_002347"
                     /EC_number="1.8.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418007.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin sulfoxide reductase"
                     /protein_id="HFO5819210.1"
                     /translation="MANSSSRYSVLTAAHWGPMLVETDGETVFSSRGALATGMENSLQ
                     SAVRDQVHSNTRVRFPMVRKGFLASPENPQGIRGQDEFVRVSWDEALELIHHQHKRIR
                     EAYGPASIFAGSYGWRSNGVLHKASTLLQRYMALAGGYTGHLGDYSTGAAQAIMPYVV
                     GGSEVYQQQTSWPLVLEHSDVMVLWSANPLNTLKIAWNASDEQGLSYFSALRDSGKKL
                     ICIDPMRSETIDFFGDKMEWVAPHMGTDVALMLGIAHTLVENGWHDEAFLARCTTGYA
                     VFASYLLGESDGIAKNAEWAAEICGVGAAKIRELAAIFHQNTTMLMAGWGMQRQQFGE
                     QKHWMLVTLAAMLGQIGTPGGGFGLSYHFANGGNPTRRAAVLSSMQGSLPGGTDAVDK
                     IPVARIVEALENPGGAYQHNGMDRHFPDIRFIWWAGGANFTHHQDTNRLIRAWQKPEL
                     VVISECFWTAAAKHADIVLPATTSFERNDLTMTGDYSNQHLVPMKQVVPPRYEARNDF
                     DVFAELSERWEKGGYARFTEGKSELQWLETFYNVARQRGASQQVELPPFAEFWQANQL
                     IEMPENPDSERFIRFADFRRDPQAHPLKTASGKIEIFSQRIADYAYPDCPGHPMWLEP
                     DEWQGNAEPEQLQVLSAHPAHRLHSQLNYSSLRELYAVANREPVTIHPDDAQARGIQD
                     GDTVRLWNARGQILAGAVISEGIKPGVICIHEGAWPDLDLTADGICKNGAVNVLTKDL
                     PSSRLGNGCAGNTALAWLEKYNGPELTLTAFEPPASS"
     gene            complement(6727..7167)
                     /locus_tag="ACHKBL_002348"
     CDS             complement(6727..7167)
                     /locus_tag="ACHKBL_002348"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HFO5819211.1"
                     /translation="MIREVQHSELPAILELWLESTTWGHPFIKSSYWRDCIPLVRDAY
                     LANAQNWVWEEDGKLLGFVSIMEGRFLAAMFVAPKAVRRGIGKALMQYVQQRHPHLML
                     EVYQKNQPAIDFYQAQGFHIVDCAWQDETQLPTWIMSWPVVQTL"
     gene            complement(7164..7727)
                     /gene="tag"
                     /locus_tag="ACHKBL_002349"
     CDS             complement(7164..7727)
                     /gene="tag"
                     /locus_tag="ACHKBL_002349"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HFO5819212.1"
                     /translation="MERCGWVSQDPLYIAYHDNEWGVPETDSKKLFEMICLEGQQAGL
                     SWITVLKKRENYRASFHQFDPVKVAAMQEEDVERLVQDAGIIRHRGKIQAIIGNARAY
                     LQMEQNGEPFADFVWSFVNHQPQVTQATTLSEIPTSTPASDALSKALKKRGFKFVGTT
                     ICYSFMQACGLVNDHVVGCCCHPGNKP"
     gene            7885..8583
                     /locus_tag="ACHKBL_002350"
     CDS             7885..8583
                     /locus_tag="ACHKBL_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HFO5819213.1"
                     /translation="MIIKKSGGRWQLSLLASVVISAFFLNTAYAWQQEYIVDTQPGHS
                     TERYTWDSDHQPDYNDILSQRIQSSQRALGLEVNLAEETPVDVTSSMSMGWNFPLYEQ
                     VTTGPVAALHYDGTTTSMYNEFGDSTTTLADPLWHASVSTLGWRVDSRLGDLRPWAQI
                     SYNQQFGENIWKAQSGLSRMTATNQNGNWLDVTVGADMLLNQNIAAYAALSQAENTTN
                     NSDYLYTMGVSARF"
     gene            8812..10020
                     /locus_tag="ACHKBL_002351"
     CDS             8812..10020
                     /locus_tag="ACHKBL_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO5819214.1"
                     /translation="MTPSNYQRTRWLTLIGTIITQFALGSVYTWSLFNGALSAKLDAP
                     VSQVAFSFGLLSLGLAISSSVAGKLQERFGVKRVTMASGILLGLGFFLTAHSDNLMML
                     WLSAGVLVGLADGAGYLLTLSNCVKWFPERKGLISAFAIGSYGLGSLGFKFIDTQLLE
                     TVGLEKTFVIWGAIALVMIVFGATLMKDAPKQEVKTSNGVVEKDYTLAESMRKPQYWM
                     LAVMFLTACMSGLYVIGVAKDIAQSLAHLDAISAANAVTVISIANLSGRLVLGILSDK
                     IARIRVITIGQVISLVGMAALLFAPLNAVTFFAAIACVAFNFGGTITVFPSLVSEFFG
                     LNNLAKNYGVIYLGFGIGSICGSIIASLFGGFYVTFYVIFALLILSLALSTTIRQPEQ
                     KMLREAHGSL"
     gene            10344..12035
                     /gene="eptB"
                     /locus_tag="ACHKBL_002352"
     CDS             10344..12035
                     /gene="eptB"
                     /locus_tag="ACHKBL_002352"
                     /EC_number="2.7.8.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid A phosphoethanolamine
                     7''-transferase"
                     /protein_id="HFO5819215.1"
                     /translation="MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWK
                     GISAVVELAATVLVTFFLLRLLSLFGRRSWRILASLVVLFSAGASYYMTFLNVVIGYG
                     IIASVMTTDIDLSKEVVGLNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRS
                     LAVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAW
                     ARVDESSDNNSLLNPAKKFTYQAPQNVDDTYVVFIIGETTRWDHMGIFGYERNTTPKL
                     AQEKNLAAFRGYSCDTATKLSLRCMFVRQGGAEDNPQRTLKEQNIFAVLKQLGFSSDL
                     YAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLI
                     ILHTKGSHFNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVID
                     QVRDKKAIVFYAADHGESINEREHLHGTPRELAPPEQFRVPMMVWMSDKYLENPVNAQ
                     AFAQLKKEADMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCHIPQAREAAAN"
     gene            12127..12203
                     /locus_tag="ACHKBL_002353"
     tRNA            12127..12203
                     /locus_tag="ACHKBL_002353"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12161..12163,aa:Pro,seq:cgg)
     gene            12238..12371
                     /locus_tag="ACHKBL_002354"
     ncRNA           12238..12371
                     /ncRNA_class="other"
                     /locus_tag="ACHKBL_002354"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            13116..14723
                     /gene="dppA"
                     /locus_tag="ACHKBL_002355"
     CDS             13116..14723
                     /gene="dppA"
                     /locus_tag="ACHKBL_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter substrate-binding
                     protein DppA"
                     /protein_id="HFO5819216.1"
                     /translation="MRISLKKSGMLKLGLSLVAMTVAASVQAKTLVYCSEGSPEGFNP
                     QLFTSGTTYDASSVPLYNRLVEFKIGTTEVIPGLAEKWEVSEDGKTYTFHLRKGVKWH
                     DNKEFKPTRELNADDVVFSFDRQKNAQNPYHKVSGGSYEYFEGMGLPELISEVKKVDD
                     NTVQFVLTRPEAPFLADLAMDFASILSKEYADAMMKAGTPEKLDLNPIGTGPFQLQQY
                     QKDSRIRYKAFDGYWGTKPKIDTLVFSITPDASVRYAKLQKNECQVMPYPNPADIARM
                     KQDKSINLMEMPGLNVGYLSYNVQKKPLDDVKVRQALTYAVNKDAIIKAVYQGAGVSA
                     KNLIPPTMWGYNDDVQDYTYDPEKAKALLKEAGLEKGFSIDLWAMPVQRPYNPNARRM
                     AEMIQADWAKVGVQAKIVTYEWGEYLKRAKDGEHQTVMMGWTGDNGDPDNFFATLFSC
                     AASEQGSNYSKWCYKPFEDLIQPARATDDHNKRVELYKQAQVVMHDQAPALIIAHSTV
                     FEPVRKEVKGYVVDPLGKHHFENVSIE"
     gene            complement(14786..15013)
                     /locus_tag="ACHKBL_002356"
     CDS             complement(14786..15013)
                     /locus_tag="ACHKBL_002356"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5819217.1"
                     /translation="MTRSLELRVCIAHKSIPALVEQGMKYCCRMALHAEAQRHRVCRL
                     LTKSALFMPDAAQTPYPTYKIVQTQYIAEFS"
     gene            15031..16050
                     /gene="dppB"
                     /locus_tag="ACHKBL_002357"
     CDS             15031..16050
                     /gene="dppB"
                     /locus_tag="ACHKBL_002357"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppB"
                     /protein_id="HFO5819218.1"
                     /translation="MLQFILRRLGLVIPTFIGITLLTFAFVHMIPGDPVMIMAGERGI
                     SPERHAQLLAELGLDKPMWQQYLHYIWGVMHGDLGISMKSRIPVWEEFVPRFQATLEL
                     GVCAMIFATAVGIPVGVLAAVKRGSIFDHTAVGLALTGYSMPIFWWGMMLIMLVSVHW
                     NLTPVSGRVSDMVFLDDSNPLTGFMLIDTAIWGEDGNFIDAVAHMILPAIVLGTIPLA
                     VIVRMTRSSMLEVLGEDYIRTARAKGLTRLRVIIVHALRNAMLPVVTVIGLQVGTLLA
                     GAILTETIFSWPGLGRWLIDALQRRDYPVVQGGVLLVATMIILVNLLVDLLYGVVNPR
                     IRHKK"
     gene            16060..16962
                     /gene="dppC"
                     /locus_tag="ACHKBL_002358"
     CDS             16060..16962
                     /gene="dppC"
                     /locus_tag="ACHKBL_002358"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppC"
                     /protein_id="HFO5819219.1"
                     /translation="MSQVTENKVISAPVPMTPLQEFWHYFKRNKGAVVGLVYVVIVLF
                     IAIFANWIAPYNPAEQFRDALLAPPAWQEGGSMAHLLGTDDVGRDVLSRLMYGARLSL
                     LVGCLVVVLSLIMGVILGLIAGYFGGLVDNIIMRVVDIMLALPSLLLALVLVAIFGPS
                     IGNAALALTFVALPHYVRLTRAAVLVEVNRDYVTASRVAGAGAMRQMFINIFPNCLAP
                     LIVQASLGFSNAILDMAALGFLGMGAQPPTPEWGTMLSDVLQFAQSAWWVVTFPGLAI
                     LLTVLAFNLMGDGLRDALDPKLKQ"
     gene            16973..17956
                     /gene="dppD"
                     /locus_tag="ACHKBL_002359"
     CDS             16973..17956
                     /gene="dppD"
                     /locus_tag="ACHKBL_002359"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HFO5819220.1"
                     /translation="MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGS
                     GKSVSSLAIMGLIDYPGRVMAEKLEFNGQDLQRISEKERRNLVGAEVAMIFQDPMTSL
                     NPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVA
                     EAAHKIIVMYAGQVVETGDAHAIFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPG
                     KYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLDDAGRPTL"
     gene            17953..18957
                     /gene="dppF"
                     /locus_tag="ACHKBL_002360"
     CDS             17953..18957
                     /gene="dppF"
                     /locus_tag="ACHKBL_002360"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding subunit
                     DppF"
                     /protein_id="HFO5819221.1"
                     /translation="MSTQEATSQQPLLQAIDLKKHYPVKKGMFAPERLVKALDGVSFN
                     LERGKTLAVVGESGCGKSTLGRLLTMIETPTGGELYYQGQDLLKHDPQAQKLRRQKIQ
                     IVFQNPYGSLNPRKKVGQILEEPLLINTSLSKEQRREKALSMMAKVGLKTEHYDRYPH
                     MFSGGQRQRIAIARGLMLDPDVVIADEPVSALDVSVRAQVLNLMMDLQQELGLSYVFI
                     SHDLSVVEHIADEVMVMYLGRCVEKGTKDQIFNNPRHPYTQALLSATPRLNPDDRRER
                     IKLTGELPSPLNPPPGCAFNARCRRRFGPCTQLQPQLKDYGGQLVACFAVDQDENPQR
                     "
     gene            complement(18987..20258)
                     /locus_tag="ACHKBL_002361"
     CDS             complement(18987..20258)
                     /locus_tag="ACHKBL_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transport family protein"
                     /protein_id="HFO5819222.1"
                     /translation="MQHNTLPKHDQKMPFTRYDFGWVLLCIGMAIGAGTVLMPVQIGL
                     KGIWVFITAAIIAYPATWVVQDIYLKTLSESDSCNDYTDIISHYLGKNWGIFLGVIYF
                     LMIIHGIFIYSLSVVFDSASYLKTFGLTDADLSQSLLYKVAIFAVLVAIASGGERLLF
                     KISGPMVVVKVGIIVVFGFAMIPHWNFANITAFPQASVFFRDVLLTIPFCFFSAVFIQ
                     VLNPMNIAYRKREADKVLATRLALRTHRISYVTLIAVILFFAFSFTFSISHEEAVSAF
                     EQNISALALAAQVIPGHIIHITSTVLNIFAVLTAFFGIYLGFHEAIKGIILNLLSRII
                     DTKKINSRMLTLAICAFIVITLTIWVSFRVSVLVFFQLGSPLYGIVSCLIPFFLIYKV
                     AQLEKLRGFKAWLILLYGILLCLSPLLKLIE"
BASE COUNT         4909 a         5057 c         5415 g         5156 t
ORIGIN      
        1 aattttatga gattttttta ggattatatc aaggagaaga aacgaactta ttaagctaga
       61 atagctgtgg gtgcttgaga ctgtttgtct caggtattca ccgaaaggca gacagagaaa
      121 agccccacct gactataaat caaagtgagg ctaccctatg cctgaacacc ataaggttag
      181 cctcttactc gttggaaatc aacacaggag gctgggaatg ccgcagaaat atagattgct
      241 ttctttaata gtgatttgtt tcacgctttt attttttacc tggatggtaa gagattcact
      301 gtgtgaattg catattaaac agggaagtta tgagctggcg gcatttttag cctgcaattt
      361 gaaagagtaa gagactttgg cgggaagtta ttcccgcctt tcttacggcg ttgcgcattc
      421 tcattgcacc caaatttatt cttcacaaaa ataacactag attctattac gcgatcgatt
      481 atttatttcc tgaaaaacaa ataaaaaaat ccccgccaaa tggcagggat cttagattct
      541 gtgcttttaa gcagagatta caggctggtt acgttaccag ctgccgggcc tttagcgccg
      601 ctttcgatgg tgaaggacac tttctgacct tcgtccagag atttgtaacc atcgttctgg
      661 atagcagaga agtgtacgaa cacgtcttta gagccatcgt caggagtgat gaagccgaag
      721 cctttgtcag cgttgaacca ttttacgata ccagtcattt taccggacat agtgtattac
      781 ctttaataat taagtgtgcc tttcggcgat atggcgtgct ttacagattt tgaagcgtta
      841 aaggaatgtg cactacgagg ggtatcaacg ataactcttg aagggacttg ccttactaca
      901 ctgctttaat ggtctgtacg tcaaaccgtt gatgtgcatt aagccacgca ttggcgggtg
      961 atgcaacaat tatttttcat atttatgatt aatcggttgg aaaacggtcc tgtcatcagg
     1021 accgtaaaca gcaataaagt ggataaaagt ctattccatc aactgcttac ttaatgccgg
     1081 gttggcttga atcaaacgca tcaattttag ttcggcactt gaaggcttca cgcgtctgga
     1141 ttcccattcc tttaccatgg cgactgatac gcccaaaacc cgggcgaaat catcgatttt
     1201 taaccctgtc ccttttcgta actgctcaat ttcggtacag gacgttgttc tgtgcgtcag
     1261 ggtaattttc tgcgtttcat ctttaaaaac aatctgttcc aggctgctca acagctcatg
     1321 cattggatct ttatattcca ttgaaaactc ctcaaatcac actgcgggat cgtgaattgc
     1381 atcgaagctc attaagaata gtcgggaaaa acagacatag cggggtgtag tcagggcatg
     1441 ctctggctat gattcgattc agccagtcat ttagcgtgac ttatagccac ctaacgatct
     1501 gataaatgcc gatatatctc ttaccactcc cggtttgtaa agatttcgaa aatctaacgt
     1561 tctgcgaagg cgtaaatctc aggctacaaa aaaagcaacg gcactatcat tttttaacaa
     1621 tgaaacaaca aattgacctt gtttcatttt ctccatgatt ttaccaggaa gattacgtag
     1681 aaagggtatc ctgcaagcct gtcctggact atccttgtca cgtcagacac gcgtgtgtcg
     1741 ttgtgcgctt tttttgggtg aaaggagtaa gaaaatggcg acaggaaagt cctgctctcg
     1801 ctggtttgcg cctcttgcgg cgttattaat ggtagttagc ctgagtgggt gttttgataa
     1861 agaaggcgat cagcgtaaag cgtttatcga cttcctgcag aatacagtga tgcgtagcgg
     1921 tgaacgtcta ccaaccctga ctgccgatca gaaaaaacag tttggtcctt ttgtctctga
     1981 ttacgcgatt ctgtatggtt attctcagca ggtgaatcag gcgatggatt ccggtctgcg
     2041 cccggttgta gacagcgtta acgcaattcg cgtaccgcag gattacgtta cgcaaagcgg
     2101 cccactgcgt gaaatgaacg gttctctggg cgtactggcg caacagctgc aaaatgcgaa
     2161 gttgcaggcc gatgctgcgc actctgcgct aaaacagagc gatgacctga aaccggtctt
     2221 cgatcaggca ttcaccaaag tggtaacgac gccagctgat gcgttgcaac cgttgattcc
     2281 ggcggcacaa actttcacgc aacaactggt gatggttgga gactacattg ctcagcaagg
     2341 tactcaggtg agctttgtcg caaatggcat ccagttcccg acttcgcagc aggcaagtga
     2401 atataacaaa ctgattgcgc cattaccggc acaacatcag gcgtttaatc aggcctggac
     2461 tacagcagtt actgcaaccc aataaagagt aaaagcccga gcgattatcg ggctttactc
     2521 cacgcagtcg cggcttagtc cgcgacgtgc ggattcacac agttcttctc aacctttcct
     2581 tgtaacgcat caatcaagtt atccacggca caggcggcca tgccataacg cgtctcatga
     2641 gtggcagatc caatatgcgg tactgcgacg acgttggcca ttgagagcaa cggcgaatct
     2701 actgacagtg gctcttgttc gaagacatcc agcccggcag cgtgaatttc ccctttctgc
     2761 aatgcagcaa tcagtgcatt ttcatcaacc accgggccac gtccggcatt aatgaaaatg
     2821 gcggaggatt tcattttggc gaattgttct gcgccaaaca gatgatgcgt ttcatcagtt
     2881 aacggcagga tcaggcaaac gaaatctgac tcttgtaaca gtgtatccaa atcgcagtag
     2941 cgggcgttga agcgttcttc tgcttcttta tggtggcggc gcgcgttata gaggatgggc
     3001 atgttgaagc caaagtgcgc acgttgtgcc agcgccatgc cgatccgtcc catcccgaca
     3061 atgcccagtg ttttatggtg aacgtcagtg ccgtaccagt ccgggcctat gctcgcggtc
     3121 cattcgcctg cttttactcg ttctgctacc tccacaaccc gacgagcggt agacaatacc
     3181 agcgccatca gcgtatcggc gacggtttct gttaatacgg ttggcgtgtg catcagcaga
     3241 atttttcggg cggtaagcgc atcgacatca aaattgtcat agcctacgga gatcgttgat
     3301 gtggcacgca gtttcggcat tttttccagc aatgcggcat caacattctc gtttgaaccc
     3361 agtaaacctt cagcttcggc aagaattgct gcattttgtt cgatggtttg tgggctgagg
     3421 tttgccacct ggtgaacggt gaaatgctct tgcaggcgtt gcagtaaatc atcaggtaag
     3481 gctttgtaga ggataacgga cggcttcatt cttctctcca ttcactgata aggcgatcac
     3541 cttagcctga aatatgatga aggaaaactg aagaatgcca ggtgatttct gcatcacctg
     3601 gcatgaaagg gattacagcg ggcttaaggt aatttctaca cggcggtttt gcgccttacc
     3661 ttctgcagtg ctgttgctgg cgattgggtt agccgggcca aggccctgag tacggatgcg
     3721 gctggcgtcc acgccctggg tgatcaacgc gctggcaacg gaatccgcac gctgctggga
     3781 gagacgcatg ttcaggtcgt gcccacccgt gctgtcggta taaccaatca cgttaaccgc
     3841 cgttttcgga tactctttca gtaccattgc cacgccggtc agggtgttag cgcccgccgg
     3901 tttcagggtt gcgctgctgc tgtcgaaggt cacattgttc ggcatattga ggataatgtt
     3961 atccccgctg cgggttacgc taacaccagt gccgcgcatt ttgtcacgca gcttcgcttc
     4021 ctgcacatcc atgtaataac ccacgccgcc gcccagagct gcgcctgctg ctgcgccaat
     4081 gagcgcgcct ttaccgcgat ctttcttcga agaagagagc gcaccaatac ccgcgccaac
     4141 gagagagccc agacctgcgc cgatagcaga tttacctgct tcgcgttcgc cggtgtaagg
     4201 gttagttgta cagccagata ccgccagagc gccactcact acggcggcaa taagataaac
     4261 acgtttcttc attgttaatc cttaataacc tttttattct ttgccacggg ttctgtggcg
     4321 ggagattatg ccgcgtgaac atgaagatta ttcctgggaa tactcggaaa tttgtaagta
     4381 atatttaact gctcaataca tctaaccttt caggagtctt cggtttggcc aactcatcct
     4441 cacgatattc cgttctgact gccgcccact gggggcctat gctggttgaa accgacggcg
     4501 aaaccgtgtt tagctcgcgt ggcgcgttag ccacaggaat ggaaaactcc ttgcagagcg
     4561 cggttcgcga ccaggttcac agcaatacgc gggtgcgatt tccaatggtg cgcaaaggct
     4621 ttcttgcgtc accggaaaat ccgcaaggca ttcgtgggca ggatgaattt gttcgcgtga
     4681 gttgggatga ggcgctggag cttattcacc atcaacataa acgcattcgt gaggcttatg
     4741 gtccggcatc gatttttgct ggttcctacg gctggcgttc aaacggcgtg ctgcataagg
     4801 cctcgacatt attacaacgc tatatggcgc tggcaggcgg ttatactggg catctggggg
     4861 attattcgac cggcgcggca caggcgatca tgccgtatgt cgtgggtggc agcgaagttt
     4921 atcaacagca aaccagttgg ccgctggtgc tggaacatag cgatgtcatg gtgctgtgga
     4981 gtgctaaccc actcaatacg ctgaaaattg cgtggaatgc atccgatgag caggggcttt
     5041 cttacttttc cgcactgcgt gacagcggga aaaagctgat ctgcattgat ccaatgcgat
     5101 cggaaaccat cgatttcttt ggcgataaaa tggagtgggt ggcaccacac atgggcaccg
     5161 atgttgcgtt gatgctgggg atcgcccata cgctggtgga aaatggttgg cacgacgaag
     5221 cgtttctggc gcgttgcacc acaggttatg ccgtcttcgc ctcttatttg ctgggcgaga
     5281 gtgatggtat agcgaaaaac gccgaatggg cggcagagat ttgtggtgtt ggcgcagcga
     5341 aaatccgcga gctggcggct attttccacc aaaatacaac catgctgatg gcaggctggg
     5401 gaatgcagcg ccaacagttt ggtgagcaaa aacactggat gctcgtcacg ctggcggcaa
     5461 tgttggggca aatcggcaca cccggcggcg gttttggtct ttcttaccat tttgccaatg
     5521 gtggtaaccc cacgcgccgc gctgcggtgc tctcttccat gcagggcagt ttgcctggcg
     5581 gcaccgatgc ggtggataaa atccctgttg cccgcattgt tgaagcactg gaaaaccccg
     5641 gtggcgcata tcaacataac ggtatggacc gacatttccc ggatattcgt tttatctggt
     5701 gggcgggtgg tgccaacttt actcatcatc aggataccaa tcgcctgatc cgtgcctggc
     5761 aaaaaccgga gctggtggtg atctctgaat gcttctggac ggcggcggcg aaacacgcgg
     5821 atatcgttct gcctgcgacc acctcttttg agcgtaatga tctcaccatg accggcgatt
     5881 acagtaatca gcatctggtg ccgatgaagc aagtggtgcc gccacgctat gaagcgcgta
     5941 atgattttga tgtttttgcc gagttaagtg aacgttggga gaagggcggc tatgcgcggt
     6001 ttacggaagg aaaaagtgag ctgcaatggc tggaaacgtt ttataacgtt gcccggcaac
     6061 gcggggcaag ccagcaggtt gaattgccgc catttgctga gttctggcaa gccaaccagt
     6121 taattgagat gccggaaaac ccggacagcg agcggtttat tcgcttcgcc gattttcgcc
     6181 gcgatccgca ggcgcatcca ttaaaaaccg ccagcggcaa gattgaaatc ttctcgcagc
     6241 gtattgccga ttacgcttat ccggattgcc ctgggcatcc aatgtggctg gagccggacg
     6301 aatggcaggg caatgccgaa ccggaacagt tgcaggtact ttctgctcat ccggcacatc
     6361 gtctgcacag ccagctgaat tacagttctc tgcgcgaatt gtacgcggtg gcaaaccgtg
     6421 agcctgtcac cattcatcct gacgatgctc aggcgcgcgg catacaagat ggcgatactg
     6481 ttcggctgtg gaacgcacgc gggcaaattc ttgccggggc agtcattagc gagggaatta
     6541 aacctggcgt gatttgcatt cacgaagggg catggccgga tctggattta accgctgacg
     6601 gtatttgtaa aaacggcgca gtgaacgtgc tgaccaaaga tctccccagc tcgcggctgg
     6661 ggaatggctg tgcgggtaat acggcgctgg catggctgga aaaatacaac ggtccggaac
     6721 tgacacttac agcgtttgaa ccaccggcca gctcataatc caggtgggta gttgggtttc
     6781 atcctgccat gcgcagtcga caatgtgaaa accctgcgcc tggtaaaaat ctatcgccgg
     6841 ttgatttttt tgataaacct ccagcatcag atggggatgg cgctgctgca catactgcat
     6901 tagcgcctta ccaataccgc gtctgacggc cttcggtgcg acaaacattg cagccagaaa
     6961 tcggccttcc ataatgctga caaaaccgag aagcttaccg tcttcttccc agacccagtt
     7021 ttgcgcgttg gcaagatacg catcgcgcac cagcggaatg cagtcacgcc agtaactcga
     7081 ttttataaag ggatgccccc aggttgtact ttccagccac agttcgagga tcgcggggag
     7141 ttctgaatgt tgcacttccc gaatcatggt ttatttcccg gatggcaaca gcagccaacc
     7201 acatgatcat tcaccagccc acatgcctgc ataaaggagt aacagattgt ggtaccgaca
     7261 aacttaaaac cacgtttttt tagtgcctta gatagggcgt cggaggcggg tgtagatgtg
     7321 gggatttcgc tcaacgttgt ggcttgtgtc acctgtggtt gatgattgac aaacgaccag
     7381 acaaagtcgg caaacggttc gccgttctgt tccatttgca ggtacgcccg cgcattacca
     7441 ataattgcct gaattttccc gcgatggcgg ataatcccgg cgtcctgtac cagtctttcg
     7501 acatcctctt cctgcattgc agccaccttc accggatcga actgatgaaa gctggcgcga
     7561 tagttttcgc gttttttgag gacggtgatc cacgataatc cagcctgctg cccttcaagg
     7621 cagatcattt cgaacagttt tttactgtca gtttcaggca cgccccactc attatcatgg
     7681 taggcaatat aaagcgggtc ctgactcacc cagccgcaac gttccatact ttccctcgct
     7741 atgtacccga tcgtaaaaat ttcagccgct tttcttgacg catcggtgaa aaagtcaata
     7801 tttaatacag ggctactatg cccgatatcc ttcatacaat gacaataata ttgccgaatt
     7861 cggctcttcc ctggagtcgt gttaatgatc ataaaaaaaa gcggtggtcg ctggcagcta
     7921 agcctgctgg cgagtgtggt aatcagtgcc ttttttctca acacagctta cgcctggcaa
     7981 caagaatata tcgttgatac gcaacccgga cattccacag agcgttacac ctgggatagc
     8041 gatcatcaac ctgattacaa cgatattttg tcgcaacgta ttcaaagtag ccaaagggcg
     8101 ctgggactgg aagtcaatct ggcggaagaa actcctgtgg atgtgaccag cagtatgagt
     8161 atgggctgga attttccttt atatgagcag gttacaaccg gcccggtcgc ggcattacat
     8221 tacgatggca caaccacctc gatgtataac gagtttggcg acagtactac cacgctggcc
     8281 gatccgttat ggcatgccag cgtgagtacc ttaggctggc gtgttgactc ccggcttggc
     8341 gatctccgac cctgggcgca aatcagctat aaccagcaat ttggcgagaa tatctggaag
     8401 gcgcaatcag gcctgagccg gatgacggcg acaaaccaga acggcaactg gctggatgtc
     8461 accgtaggcg ctgatatgtt gctcaatcaa aatattgctg cctatgctgc gctatctcag
     8521 gcagaaaata ccactaacaa tagcgactat ctgtatacga tgggggttag cgccagattt
     8581 taacgttata gatacaattg aaaccaataa ataacaacta atgtggcgat agtgggtgat
     8641 aacttactaa ataataaatt tggtgaataa ttgtcgagtc attcattcct gaactaatgc
     8701 atttcattcc gttctgatgg catttcatgc cgtttttccc caggcataaa gtgcacttcg
     8761 ttatggttgt cagcagagat ttttcctttt tattactgca ggaatactgc catgacacct
     8821 tcaaattatc agcgtacccg ctggttgaca ctcatcggta ctatcattac ccagtttgcc
     8881 cttgggtcgg tttatacctg gagcctgttt aacggcgcgc tttctgccaa gctggatgcg
     8941 ccggtaagcc aggtcgcttt ctctttcggc ttgttaagtc tggggctggc aatttcatct
     9001 tctgttgcgg ggaaattaca ggaacgtttt ggcgttaaac gcgtcactat ggcttccggc
     9061 attttgctgg gattaggctt cttcctgaca gcgcattctg acaacctgat gatgctgtgg
     9121 ttaagcgccg gtgtgctggt cggtctggcg gatggcgcgg gttacctgct gacgctctct
     9181 aactgcgtga agtggttccc ggagcgtaaa ggtctgatct ccgcgttcgc tatcggttct
     9241 tatggtctgg gtagcctggg tttcaaattt atcgacacgc agctgctgga aacggtgggt
     9301 ctggaaaaaa cctttgtgat ttggggagcg attgcgctgg tgatgattgt ctttggcgca
     9361 acattaatga aagacgcacc aaaacaggaa gtgaaaacca gcaatggtgt ggtggagaag
     9421 gactacaccc tggcagagtc gatgcgtaaa ccgcagtact ggatgttagc ggttatgttc
     9481 ctgactgcgt gcatgagtgg tctgtatgtg attggtgtag cgaaagatat cgctcaaagt
     9541 ctggcgcatc ttgatgcaat ttccgcagcc aatgctgtga cggttatttc catcgccaac
     9601 ctttctggtc gtctggtgct gggcattctg tctgacaaaa tcgcccgtat ccgtgttatc
     9661 accattggtc aggtgatatc gctggtgggt atggcggccc tgctgtttgc accattgaat
     9721 gcagtgacgt tctttgcagc gattgcctgc gtggcattta actttggcgg cactatcacg
     9781 gtgttcccgt cactggtcag tgagttcttc ggcctcaata acctggcgaa aaactacggt
     9841 gtgatttatc tcggtttcgg tatcggcagc atttgtgggt cgattatcgc ctcactgttt
     9901 ggcggcttct atgtgacctt ctacgtgatt ttcgcactgc tgattctgtc actggcgctc
     9961 tctacgacga ttcgtcagcc agagcagaaa atgttgcgtg aggcgcatgg ctccctttaa
    10021 tcatctgccg cgagctactg ggctttagcc ttagtgtgaa tcaaacgctt caacgcaatg
    10081 tataaacaat gacgcgtaaa atttgggcca gttaactggc cctttttact gctttttgta
    10141 acgctaaagt ctcttttcaa acttgcattt ttgtaaattt gtgcttcatg cacactcttt
    10201 ccccacactt tttccctttg ctgtggtcta cttattcgcg cgtgtagatt ttacttatct
    10261 gactacctcc gcacttttcc cctgccgggc ctgaaaagcc gctaagcagg gtgttatcac
    10321 ctgtttgtcc agggtttgtt tgcatgagat acatcaaatc gattacacag cagaagctga
    10381 gctttttgct tgcaatctat attggccttt ttatgaatgg cgcggttttt taccgccgct
    10441 tcggcagcta tgcgcacgat tttaccgtct ggaaaggcat ttctgctgtt gttgaactgg
    10501 ccgccaccgt actggtgacc ttctttttac tacgtcttct ttcgctgttt ggccgccgta
    10561 gctggcgtat tctggcgtcg ctggtggtgc tcttttccgc aggtgccagc tattacatga
    10621 ccttccttaa tgtggtcatt ggttatggca tcatcgcttc cgtcatgacc accgatatcg
    10681 acctgtcaaa agaagttgtt ggtctgaact ttattctctg gttaatcgcc gttagcgcgt
    10741 tgcctcttat ccttatctgg aataaccgct gtcgttacac cttgctccga caactgcgaa
    10801 ccccagggca gcgtattcgc agcctggcgg tcgtcgtact ggcaggtatt atggtttggg
    10861 caccgattcg tttgctggat atccagcaga agaaagtgga gagggcgaca ggtgttgatt
    10921 tgccgagtta tggcggcgtc gtcgcgaact cttatctgcc atcaaactgg ctttcggcat
    10981 taggtcttta tgcctgggcg cgggtcgatg aatcttccga taacaattct ctccttaacc
    11041 ctgcgaagaa gtttacgtac caggctccac aaaacgttga tgatacatat gtcgttttca
    11101 ttattggtga aaccacgcgc tgggatcata tgggcatttt cggctatgaa cgcaatacca
    11161 caccgaagct ggcccaggaa aaaaatctgg cggcgttccg tggttactcc tgtgataccg
    11221 caaccaaact ctctctgcgt tgcatgtttg tacgtcaggg gggcgcggaa gataatccgc
    11281 agcgcacatt aaaagaacag aacattttcg cggtattgaa gcagttagga ttcagttctg
    11341 atctctacgc tatgcaaagc gaaatgtggt tctacagcaa tacgatggcg gacaacattg
    11401 cttatcgtga gcagattggt gcggagccac gtaaccgtgg caaaccggta gatgatatgt
    11461 tgctggtaga cgaaatgcag caatcgctgg ggcgcaaccc ggatggtaag catctgatca
    11521 ttctgcatac caaaggctca cactttaact acacccagcg ttacccgcgc agctttgcgc
    11581 agtggaagcc ggaatgtatt ggtgttgata gcggctgtac caaagcgcag atgatcaact
    11641 catacgacaa ctcggtgacc tatgtggatc actttatttc cagcgtgatt gatcaggttc
    11701 gcgataagaa agcgattgtg ttctatgcag ctgaccatgg tgagtcaatt aatgaacgcg
    11761 agcacctgca cggcacgccg cgtgaactgg caccgccgga acagttccgc gtgccgatga
    11821 tggtctggat gtcggataaa tatctggaaa atccggtcaa tgcgcaggca tttgcgcagc
    11881 tgaaaaaaga agctgacatg aaagtgccac gccgtcacgt agagctgtac gacaccatca
    11941 tgggttgtct tggctatact tcaccggatg gtggaattaa cgaaaacaac aactggtgcc
    12001 acatcccgca ggcaagagag gcggcggcta actaagaggc ctgctgactt tctcgccgat
    12061 caaaaggcat tttgctatta agggattgac gagggcgtat ctgcgcagta agatgcgccc
    12121 cgcattcggt gattggcgca gcctggtagc gcacttcgtt cgggacgaag gggtcggagg
    12181 ttcgaatcct ctatcaccga ccaaattcga aaagcctgct caacgagcag gcttttttgc
    12241 atttgtagcc tatgaggatg agaacctccg ggggcaggga ggttcgactc gagcgaagcg
    12301 agagaacgtt gcgccagcaa cggcccgcag ggcgagccac gaagtggcga gtaatcctct
    12361 atcaccgacc aaattcgaaa agcctgctca acgagcaggc ttttttgcat ctgtagctca
    12421 tgaggaagag aacccccggg ggcggggagg ttcgactcga cgtaggtctg ataagacgcg
    12481 ccagcgtcgc atcaggcaac ggcaccgcac ttattgtcgg atgcggcgtg aacgccttat
    12541 tcgatctaca caccacacat ttccctgtca aatttgcgcc ccatatctca ctatccatta
    12601 acggatttgt gacagaaacc attgtctttt tttatatttg cttaaatctt tttttgcgtt
    12661 acttatggct ggcgctggca ttttccgctg tgcgttttag cgttcatggt attaattgct
    12721 cagatatcca ccttttcctc agattttttt cattaaatgc ataaaactta atcaccaaat
    12781 gttgctaaat attaagctga ttgttctggt tgtgttggcg tagcacaaat ttctctgctg
    12841 caaatagctg tttgaagttt ttttaatctt tgtttgtgga ttctcaccgt tggtgtaaat
    12901 cccggttggc tgtattgacg ttttcacatt ctgttgacag attgtaggtc acgaggggca
    12961 ttttatggag gatccgcact gttacactga tgttaattag tacggcatcc ccacctcata
    13021 acgttgaccc gaccgggcaa aaaacaaaaa aggtcaggca gcgacaaccc actgcaaagg
    13081 gttaaaacaa caaacatcac aattggagca gaataatgcg tatttccttg aaaaagtcag
    13141 ggatgctgaa gcttggtctc agcctggtgg ctatgaccgt cgcagcaagt gttcaggcta
    13201 aaactctggt ttattgctca gaaggatctc cggaagggtt taacccgcag ctgtttacct
    13261 ccggcaccac ctatgacgcc tcttccgtac cgctttataa ccgtctggtt gaatttaaaa
    13321 tcggcaccac tgaagtgatc ccgggcctcg ctgaaaagtg ggaagtcagc gaagacggta
    13381 aaacctatac cttccatctg cgtaaaggtg tgaagtggca cgacaataaa gaattcaaac
    13441 cgacgcgcga actgaatgcc gatgacgtag tgttctcgtt cgatcgtcag aaaaacgcgc
    13501 aaaacccgta ccataaagtc tctggcggca gctacgaata cttcgaaggc atgggcttac
    13561 cagagctgat cagcgaagtg aaaaaggtgg acgataacac cgttcagttt gtgctgactc
    13621 gcccggaagc gccgttcctc gctgacctgg caatggactt cgcctctatt ctgtcaaaag
    13681 aatatgctga cgcgatgatg aaagccggta caccagaaaa actggacctc aacccaatcg
    13741 gtaccggccc gttccagtta cagcagtacc agaaagattc ccgtattcgc tataaagcgt
    13801 ttgatggcta ctggggcacc aaaccgaaga tcgatacgct ggtcttctct attactcctg
    13861 acgcttccgt gcgttatgcg aaattgcaga agaacgaatg ccaggtgatg ccgtacccga
    13921 acccggcaga tatcgcccgc atgaagcagg ataaatccat caacctgatg gaaatgccgg
    13981 ggctgaacgt cggttatctc tcgtataacg tgcagaaaaa accactcgat gacgtgaaag
    14041 ttcgccaggc tctgacctac gcggtgaaca aagacgcgat catcaaagcg gtttatcagg
    14101 gcgcgggcgt atcagcgaaa aacctgatcc cgccaaccat gtggggctat aacgacgacg
    14161 ttcaggacta cacctacgat cctgaaaaag cgaaagcctt gctgaaagaa gcgggtctgg
    14221 aaaaaggttt ctccatcgac ctgtgggcga tgccggtaca acgtccgtat aacccgaacg
    14281 ctcgccgcat ggcggagatg attcaggcag actgggcgaa agtcggcgtg caggccaaaa
    14341 ttgtcaccta cgaatggggt gagtacctca agcgtgcgaa agatggcgag caccagacgg
    14401 taatgatggg ctggactggc gataatgggg atccggataa cttcttcgcc accctgttca
    14461 gctgcgccgc ctctgaacaa ggctccaact actcaaaatg gtgctacaaa ccgtttgaag
    14521 atctgattca accggcgcgt gctaccgacg accataacaa acgcgttgaa ctgtacaagc
    14581 aagcgcaggt ggtgatgcac gatcaggctc cggcactgat catcgctcac tccaccgtgt
    14641 ttgaaccggt acgcaaagaa gttaaaggct atgtggttga tccattaggc aaacatcact
    14701 tcgaaaacgt ctctatcgaa taattaaaag ccatacaaga ctgatggcaa aggcaaaact
    14761 gcctgatgcg ctccgcttat caggcctacg aaaactctgc aatgtattga gtttgcacga
    14821 ttttgtaggt cggataaggc gtttgcgccg catccggcat aaacaaagcg ctctttgtca
    14881 acaatctgca tacccggtgg cgctgtgcct ctgcgtgcag cgccatccgg cagcaatact
    14941 tcattccctg ttccacaagg gcgggaattg atttgtgagc aatacagaca cgcagttcca
    15001 ggctgcgggt cattatagag aatccgggtt atgttgcagt ttattctccg acgtttggga
    15061 ctcgtcatcc ccacgtttat cggtattacc cttctcacat ttgcctttgt ccacatgatc
    15121 ccgggcgatc cggtgatgat catggcgggc gagcgtggga tctccccaga gcgtcacgcg
    15181 cagctgctgg ctgaactcgg cttagataaa ccgatgtggc agcagtatct ccattacatt
    15241 tggggcgtaa tgcacggcga tttaggcatt tcgatgaaaa gccgcatccc ggtatgggaa
    15301 gagttcgtgc cgcgcttcca ggccacgctg gaacttggcg tctgcgcgat gatttttgct
    15361 acggcagtcg gtattccggt tggtgtgctg gctgcggtta aacgcggttc cattttcgat
    15421 cacacggcgg ttggcctggc gctgacaggt tattcaatgc ctatcttctg gtggggcatg
    15481 atgctgatca tgctggtttc ggtgcactgg aacctgacgc ccgtctccgg tcgcgtgagc
    15541 gacatggtgt tcctcgatga ttccaatccg ttaaccggtt ttatgctgat cgacaccgcc
    15601 atctggggtg aagacggtaa ctttatcgat gccgtcgccc atatgatctt gcccgccatt
    15661 gtgctgggta ctatcccgct ggcggtcatt gtgcgtatga cacgctcctc gatgctggaa
    15721 gtgctgggtg aagattacat ccgcaccgcg cgcgccaaag ggttgacccg ccttagggtg
    15781 attatcgtcc atgcgctgcg taacgcgatg ctgccggtgg tgaccgttat cggcctgcag
    15841 gtgggaacgt tgctggcggg ggcgattctg accgaaacca tcttctcgtg gcccggtctg
    15901 gggcgctggt tgattgacgc actgcaacgc cgcgactatc cggtagtgca gggcggcgta
    15961 ttgctggtgg cgacgatgat tatcctcgtc aacttgctgg tcgatctgct gtacggcgtg
    16021 gtgaacccgc gtattcgtca taagaagtaa ggggacatca tgtcacaggt tactgaaaat
    16081 aaagtgatta gcgcaccggt gccgatgacc ccgttacagg agttctggca ctattttaaa
    16141 cgcaacaaag gcgcggtcgt cgggctggtt tacgtcgtca tcgtgctgtt tatcgcgatc
    16201 tttgccaact ggattgcacc ctataacccg gcggaacagt tccgtgatgc actgctcgcc
    16261 ccgccagcct ggcaggaagg cggcagcatg gcgcacttgc tgggcaccga tgacgtaggc
    16321 cgtgatgtgc tgtcgcgtct gatgtacggt gcgcgattgt cactgctggt tggctgtctg
    16381 gtagttgtgt tatcgctgat tatgggcgtt attctcggcc tgatcgccgg ttacttcggc
    16441 ggcctggtcg ataacatcat catgcgcgtg gtcgatatca tgctggcgct gccaagtctg
    16501 ctgttggcac tggtgctggt ggcgattttc ggcccgtcga ttggtaacgc cgcgctggcg
    16561 ctgacctttg ttgccctgcc gcactatgtg cgcttaaccc gcgccgccgt gctggtggaa
    16621 gttaaccgcg attacgttac cgcgtctcgc gtggcaggtg ccggggcgat gcgtcagatg
    16681 tttattaaca tcttcccgaa ctgccttgcg cctctgattg ttcaggcgtc gctcggtttc
    16741 tctaacgcca ttctcgatat ggctgctctt ggtttcctcg gcatgggggc gcagccgcca
    16801 acgccggagt ggggcaccat gctctccgac gtgttgcagt tcgcgcaaag cgcctggtgg
    16861 gtcgtgacct tcccgggtct ggcgatcctg ctgacggtgc tggcatttaa cctgatgggt
    16921 gacggtctgc gtgacgcgct cgatcccaaa ctgaagcagt aagaggttcg agatggcgtt
    16981 attaaatgta gataaattat cggtgcattt cggcgacgaa agcgcaccgt tccgcgccgt
    17041 agaccgcatc agctacagcg taaaacaggg cgaagtggtc gggattgtgg gtgagtccgg
    17101 ctctggtaaa tcggtcagtt cgctggcgat tatggggctg attgattatc cgggccgcgt
    17161 aatggcggaa aagctggagt ttaacggtca ggatttgcag cgtatctcgg aaaaagagcg
    17221 ccgcaacctt gtgggtgccg aagtggcgat gatcttccag gacccgatga ccagccttaa
    17281 cccgtgctac accgtgggtt tccagattat ggaagcgatt aaggtgcatc agggcggtaa
    17341 taagagcact cgtcgtcagc gggcgattga cttgttgaat caggtcggta ttcccgatcc
    17401 ggcttcgcgt ctggatgttt acccgcatca gctttccggc ggcatgagcc agcgcgtgat
    17461 gattgccatg gcgattgcct gtcggccaaa actgctgatt gccgatgaac caaccactgc
    17521 gctggacgtg accattcagg cgcaaatcat tgaactactg ctggagctac agcagaaaga
    17581 gaacatggcg ctggtgttaa ttacccatga cctggcgctg gtggcggaag cggcacataa
    17641 aatcatcgtg atgtatgccg gtcaggtggt ggaaacgggc gatgcgcacg ccatcttcca
    17701 tgcgccgcgt cacccgtata ctcaggcatt gctgcgtgcg ctgccggaat ttgctcagga
    17761 taaagaacgt ctggcgtcgt tgccaggtgt tgttcccggc aagtatgacc gcccgaacgg
    17821 ctgcctgctt aacccgcgct gcccctatgc cactgacaga tgtcgcgctg aagaaccggc
    17881 gctgaatatg cttgctgacg ggcgtcagtc caaatgccat tacccacttg atgatgccgg
    17941 gaggccgaca ctatgagtac gcaagaggcc acctcgcaac aaccgctgtt gcaggctatc
    18001 gacctgaaaa aacattatcc ggtgaagaaa ggtatgttcg cgccggaacg tttggttaaa
    18061 gcgctggacg gcgtttcgtt taaccttgaa cgcggcaaaa cgctggcggt agtaggggaa
    18121 tctggctgcg gtaaatcgac cctcggtcgg ttgctgacga tgatcgaaac gcccaccggt
    18181 ggtgaactgt attaccaagg gcaggatctg ctcaagcacg atccgcaggc gcagaagctg
    18241 cgtcggcaga aaatccagat tgtcttccag aacccttacg gttcgctgaa tccacgtaaa
    18301 aaagtcgggc aaattcttga ggagccgctg ctgattaata ccagcttaag caaagaacag
    18361 cgtcgggaaa aagccctgtc gatgatggcg aaagtcggcc tgaaaaccga acattatgac
    18421 cgctatccgc atatgttctc cggcggtcag cgtcagcgta tcgccattgc ccgaggtctg
    18481 atgctcgacc cggatgtggt gattgccgat gagccggttt ccgcgctgga cgtctcagtg
    18541 cgcgcgcagg tgctgaatct gatgatggat ttgcagcagg agttggggct gtcttatgtc
    18601 tttatctcgc acgacctgtc ggtggtagag cacattgctg atgaagtgat ggtgatgtac
    18661 ctgggccgct gcgtggagaa gggaacgaag gaccaaatct tcaataaccc gcgtcatccg
    18721 tacactcagg cgctactctc cgcgacgccg cgcctgaacc cggacgatcg ccgcgagcgc
    18781 atcaagctca ccggtgaact gccaagccca ctgaatccac cgccgggctg cgcgtttaac
    18841 gcccgctgtc gtcggcgctt cggcccctgc acccagttgc agccgcagct aaaagactac
    18901 ggcggtcaac tggtagcttg ttttgctgtt gatcaggatg aaaatccgca gcgttaactc
    18961 acaaaaccgg ggccgtgcgc tccggtttac tcaatcagct tcaacagtgg cgacaagcat
    19021 agcaaaatgc cgtaaagcag aatcagccag gctttaaacc cgcgaagttt ttccagttgt
    19081 gcgactttat agatcaggaa aaatggaatg aggcacgaca caatgccata taacgggctt
    19141 cccaactgaa agaacaccag caccgataca cgaaacgaga cccaaatcgt caacgtaatg
    19201 acgataaaag cgcagatcgc cagagtcagc atgcgtgagt taattttctt ggtatcaata
    19261 attcggctta acagattgag aataatgcct ttaatggcct cgtggaaacc gagataaatg
    19321 ccaaagaatg cggtcagtac ggcaaagata ttcagcaccg tcgaggtgat atgaatgata
    19381 tgcccaggga tcacctgcgc ggccagtgcc agtgccgaga tattttgttc aaaggcagaa
    19441 acggcttctt cgtggctaat tgagaaggta aacgaaaagg caaaaaatag gatcaccgcg
    19501 atgagcgtga cataactaat tcggtgggta cgcagcgcga gccgggttgc cagtacttta
    19561 tccgcttccc gtttgcggta ggcaatattc atcgggttta gcacctgaat aaataccgca
    19621 gaaaagaagc aaaaaggaat agtaagcagg acgtcgcgga agaagacgga ggcttgcggg
    19681 aaggcggtta tattggcgaa attccagtgc gggatcatcg caaaaccgaa gacgacaata
    19741 atccctactt tgaccaccac cattggcccg gaaatcttaa acagtaatcg ttcaccacca
    19801 gacgcaattg ccaccagtac ggcgaaaatg gcgactttat aaagtagaga ttgtgaaaga
    19861 tcggcatcgg ttaaaccgaa ggttttcagg tacgaggcgc tgtcgaaaac cacggagaga
    19921 gagtagataa aaatcccgtg gataatcatc aaaaagtaga taaccccgag gaaaattccc
    19981 cagttcttcc ccaggtaatg actgataata tcggtgtagt cattacagga atcgctttca
    20041 gaaagggttt ttaaataaat gtcctgcact acccaggtgg caggataagc aatgatcgct
    20101 gcggtaataa atacccaaat tcccttcaag ccaatttgta ctggcatcag cacggttccg
    20161 gcaccaatcg ccatgcctat gcataataaa acccagccga agtcgtagcg tgtaaacggc
    20221 attttctgat cgtgtttcgg tagtgtgttg tgctgcataa agtcctgtcc agaaagaagt
    20281 cggcctcgca aaaatgaata aacccagttt ctcttgcgca acaggtggag aagtcgaata
    20341 cgcagtatgt aaaaaatgag gcaacagatt cactggggag gtcgcgagga ggcgcaattc
    20401 tacaaaattg cgcatttcac taccagtctg gagtcagccc ggatatgaac tgcaactaat
    20461 gcaaataaag aaattaagct ggaaggcatc acgcaatgag gaagattgcc gggtgctgac
    20521 ggtaaaaagc aatcgta
//
LOCUS       DAZFLD010000076        20490 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.076, whole genome shotgun sequence.
ACCESSION   DAZFLD010000076 DAZFLD010000000
VERSION     DAZFLD010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20490
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.076"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..834
                     /locus_tag="ACHKBL_002362"
     CDS             <1..834
                     /locus_tag="ACHKBL_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001765455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HFO5819223.1"
                     /translation="LSILLIPCIGNAQEIKIDESWLHQSLNVIGRTDSRFGPRLTNDL
                     YPEYTVAGRKDWFDFYGYVDLPKFFGVGSHYDVGIWDEGSPLFTEIEPRFSIDKLTGL
                     NLAFGPFKEWFIANNYVYDMGDNQSSRQSTWYMGLGTDIDTGLPIKLSANIYAKYQWQ
                     NYGAANENEWDGYRFKIKYSIPLTHLFGGRLVYNSFTNFDFGSDLADKSHNNKRTSNA
                     IASSHILSLLYEHWKFAFTLRYFHNGGQWNAGEKVNFGDGPFELKNTGWGTYTTIGYQ
                     F"
     gene            complement(1051..3483)
                     /gene="metL"
                     /locus_tag="ACHKBL_002363"
     CDS             complement(1051..3483)
                     /gene="metL"
                     /locus_tag="ACHKBL_002363"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase II"
                     /protein_id="HFO5819224.1"
                     /translation="MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEYSQPDDM
                     MVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADSLISAF
                     VSDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAE
                     RAAQPQVDEGLSYPLLQQLLVQHPGKRLVVTGFISRNNAGETVLLGRNGSDYSATQIG
                     ALAGVSRVTIWSDVAGVYSADPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPV
                     SGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLIEFQVPASQDFKLAH
                     KEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLA
                     LVAMVGAGVTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQ
                     SVFRAEKRIGLVLFGKGNIGSRWLELFAREQSTLSARTGFEFVLAGVVDSRRSLLSYD
                     GLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLDFASHGF
                     HVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTIL
                     SISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAR
                     EAGYNIEPDQVRVESLVPTHCEGGSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVA
                     RFDANGKARVGVEAVREDHPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGA
                     IQSDINRLAQLL"
     gene            complement(3486..4646)
                     /gene="metB"
                     /locus_tag="ACHKBL_002364"
     CDS             complement(3486..4646)
                     /gene="metB"
                     /locus_tag="ACHKBL_002364"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HFO5819225.1"
                     /translation="MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAH
                     DYSRRGNPTRDVVQRALAELEGGAGAVLTNTGMSAIHLVTTVFLKPGDLLVAPHDCYG
                     GSYRLFDSLAKRGCYRVLFVDQGDEQALRVALAEKPKLVLVESPSNPLLRVVDIAKIC
                     HLAREVGAVSVVDNTFLSPALQNPLALGADLVLHSCTKYLNGHSDVVAGVVIAKDPDV
                     VTELAWWANNIGVTGGAFDSYLLLRGLRTLVPRMELAQRNAQAIVKYLQTQPLVKKLY
                     HPSLPENQGHEIAARQQKGFGAMLSFELDGDEQTLRRFLGGLSLFTLAESLGGVESLI
                     SHAATMTHAGMAPEARAAAGISETLLRISTGIEDGEDLIADLENGFRAANKG"
     gene            4923..5240
                     /gene="metJ"
                     /locus_tag="ACHKBL_002365"
     CDS             4923..5240
                     /gene="metJ"
                     /locus_tag="ACHKBL_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HFO5819226.1"
                     /translation="MAEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRR
                     QVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPET
                     WEY"
     gene            5300..5908
                     /locus_tag="ACHKBL_002366"
     CDS             5300..5908
                     /locus_tag="ACHKBL_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HFO5819227.1"
                     /translation="MKIRLLILSLLVSVPAFAWQPQTGDIIFQISRSSQSKAIQLATH
                     SDYSHTGMLVIRNKKPYVFEAVGPVKYTPLKQWIAHGEKGKYVVRRVEGELSVEQQQK
                     LAQTAKRYLGKPYDSSFSWSDDRQYCSEVVWKVYQNALGMRVGKQQKLKEFDLSNPQV
                     QAKLKERYGKNIPLEETVVSPQAVFDAPQLTTVAKEWPLFSW"
     gene            complement(5969..6181)
                     /gene="rpmE"
                     /locus_tag="ACHKBL_002367"
     CDS             complement(5969..6181)
                     /gene="rpmE"
                     /locus_tag="ACHKBL_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HFO5819228.1"
                     /translation="MKKDIHPKYEEITASCSCGNVMKIRSTVGHDLNLDVCSKCHPFF
                     TGKQRDVATGGRVDRFNKRFNIPGSK"
     gene            6384..8582
                     /gene="priA"
                     /locus_tag="ACHKBL_002368"
     CDS             6384..8582
                     /gene="priA"
                     /locus_tag="ACHKBL_002368"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HFO5819229.1"
                     /translation="MPVAHVALPVPLPRTFDYLLPEGMTVKAGCRVRVPFGKQQERIG
                     VVVSVSNVSELPLNELKAVVEVLDVEPVFTHSVWRLLLWATDYYHHPIGDVLFHALPI
                     LLRQGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQGKIWRDQVATLEF
                     NDAALQALRKKGLCDLASETPEFSDWRKNYAVSGERLRLNTEQATAVGAIHSAADTFS
                     AWLLAGVTGSGKTEVYLSVLENVLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVL
                     HSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWR
                     YHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVL
                     DLKGQKVQAGLAPALITRMRQHLQADNQVILFLNRRGFAPALLCHDCGWIAECPRCDH
                     YYTLHQAQQHLRCHHCDSQRPVPRQCPSCGSTHLVPVGLGTEQLEQTLAPLFPGVPIS
                     RIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDGALFSA
                     DFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAE
                     RRMMQLPPWTSHVIVRAEDHNNQHAPLFLQQLRNLILSSPLADDKLWVLGPVPALAPK
                     RGGRWRWQILLQHPSRVRLQHIINGTLALINTIPDSRKVKWVLDVDPIEG"
     gene            8738..9763
                     /gene="cytR"
                     /locus_tag="ACHKBL_002369"
     CDS             8738..9763
                     /gene="cytR"
                     /locus_tag="ACHKBL_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CytR"
                     /protein_id="HFO5819230.1"
                     /translation="MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRV
                     EKAAREVGYLPQPMGRNVKRNESRTILVIVPDICDPFFSEIIRGIEVTAANHGYLVLI
                     GDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELE
                     LPTVHIDNLTAAFDAVNYLYEQGHNRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVD
                     PQYIARGDFTFEAGSKAMQQLLDLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLS
                     IIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIR
                     GSTRALP"
     gene            9855..10814
                     /gene="ftsN"
                     /locus_tag="ACHKBL_002370"
     CDS             9855..10814
                     /gene="ftsN"
                     /locus_tag="ACHKBL_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HFO5819231.1"
                     /translation="MAQRDYVRRSQPAPSRRKKSTSRKKQRNLPAVSPAMVAIAAAVL
                     VTFIGGLYFITHHKKEESETLQSQKVTGNGLPPKPEERWRYIKELESRQPGVRAPTEP
                     SAGGEVKTPEQLTPEQRQLLEQMQADMRQQPTQLVEVPWNEQTPEQRQQTLQRQRQAQ
                     QLAEQQRLAQQSRTTEQSWQQQTRTSQAAPVQAQPRQSKPASTQQPYQDLLQTPAHTT
                     AQSKPQQAAPVARAADAPKPTAEKKDERRWMVQCGSFRGAEQAETVRAQLAFEGFDSK
                     ITTNNGWNRVVIGPVKGKENADSTLNRLKIAGHTNCIRLAAGG"
     gene            10907..11437
                     /gene="hslV"
                     /locus_tag="ACHKBL_002371"
     CDS             10907..11437
                     /gene="hslV"
                     /locus_tag="ACHKBL_002371"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HFO5819232.1"
                     /translation="MTTIVSVRRNGHVVIAGDGQATLGNTVMKGNVKKVRRLYNDKVI
                     AGFAGGTADAFTLFELFERKLEMHQGHLVKAAVELAKDWRTDRMLRKLEALLAVADET
                     ASLIITGNGDVVQPENDLIAIGSGGPYAQAAARALLENTELSAREIAEKALDIAGDIC
                     IYTNHFHTIEELSYKA"
     gene            11447..12778
                     /gene="hslU"
                     /locus_tag="ACHKBL_002372"
     CDS             11447..12778
                     /gene="hslU"
                     /locus_tag="ACHKBL_002372"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HFO5819233.1"
                     /translation="MSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEEL
                     RHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAKNNWGQTEQQQEPSAARQAFR
                     KKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKLKI
                     KDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREG
                     VQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQA
                     LTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGA
                     RRLHTVLERLMEEISYDASDLSGQTITIDADYVSKHLDALVADEDLSRFIL"
     gene            12845..13771
                     /gene="menA"
                     /locus_tag="ACHKBL_002373"
     CDS             12845..13771
                     /gene="menA"
                     /locus_tag="ACHKBL_002373"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HFO5819234.1"
                     /translation="MTEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPL
                     VALLALITAGLLQILSNLANDYGDAVKGSDKPDRIGPLRGMQKGVITQQEMKRALIIT
                     VVLICLSGLALVAVACHTLADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLV
                     FFGWLSVMGSWYLQAHTLIPALILPATACGLLATAVLNINNLRDINSDRENGKNTLVV
                     RLGAVNARRYHACLLMGSLVCLALFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDP
                     VAMRPMLERTVKGALLTNLLFVLGIFLSQWAA"
     gene            13864..14349
                     /gene="rraA"
                     /locus_tag="ACHKBL_002374"
     CDS             13864..14349
                     /gene="rraA"
                     /locus_tag="ACHKBL_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008428 - ribonuclease inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0051252 - regulation of RNA metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HFO5819235.1"
                     /translation="MKYDTSELCDIYQEDVNVVEPLFSNFGGRASFGGQIITVKCFED
                     NGLLYDLLEQNGRGRVLVVDGGGSVRRALVDAELARLAVQNEWEGLVIYGAVRQVDDL
                     EELDIGIQAMAAIPVGAAGEGIGESDVRVNFGGVTFFSGDHLYADNTGIILSEDPLDI
                     E"
     gene            complement(14434..14679)
                     /gene="zapB"
                     /locus_tag="ACHKBL_002375"
     CDS             complement(14434..14679)
                     /gene="zapB"
                     /locus_tag="ACHKBL_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring assembly protein ZapB"
                     /protein_id="HFO5819236.1"
                     /translation="MTMSLEVFEKLEAKVQQAIDTITLLQMEIEELKEKNNSLSQEVQ
                     NAQHQREELERENNHLKEQQNGWQERLQALLGRMEEV"
     gene            15105..15950
                     /gene="glpF"
                     /locus_tag="ACHKBL_002376"
     CDS             15105..15950
                     /gene="glpF"
                     /locus_tag="ACHKBL_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol uptake facilitator protein GlpF"
                     /protein_id="HFO5819237.1"
                     /translation="MSQTSTLKGQCIAEFLGTGLLIFFGVGCVAALKVAGASFGQWEI
                     SVIWGLGVAMAIYLTAGVSGAHLNPAVTIALWLFACFDKRKVIPFIVSQVAGAFCAAA
                     LVYGLYYNLFFDFEQTHHIVRGSVESVDLAGTFSTYPNPHINFVQAFAVEMVITAILM
                     GLILALTDDGNGVPRGPLAPLLIGLLIAVIGASMGPLTGFAMNPARDFGPKVFAWLAG
                     WGNVAFTGGRDIPYFLVPLFGPIVGAIVGAFAYRKLIGRHLPCDICVVEEKETTTPSE
                     QKASL"
     gene            15973..17481
                     /gene="glpK"
                     /locus_tag="ACHKBL_002377"
     CDS             15973..17481
                     /gene="glpK"
                     /locus_tag="ACHKBL_002377"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HFO5819238.1"
                     /translation="MTEKKYIVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPG
                     WVEHDPMEIWATQSSTLVEVLAKADISSDQIAAIGITNQRETTIVWEKETGKPIYNAI
                     VWQCRRTAEICEHLKRDGLEDYIRSNTGLVIDPYFSGTKVKWILDHVEGSRERARRGE
                     LLFGTVDTWLIWKMTQGRVHVTDYTNASRTMLFNIHTLDWDDKMLEVLDIPREMLPEV
                     RRSSEVYGQTNIGGKGGTRIPISGIAGDQQAALFGQLCVKEGMAKNTYGTGCFMLMNT
                     GEKAVKSENGLLTTIACGPTGEVNYALEGAVFMAGASIQWLRDEMKLINDAYDSEYFA
                     TKVQNTNGVYVVPAFTGLGAPYWDPYARGAIFGLTRGVNANHIIRATLESIAYQTRDV
                     LEAMQADSGIRLHALRVDGGAVANNFLMQFQSDILGTRVERPEVREVTALGAAYLAGL
                     AVGFWQNLDELQEKAVIEREFRPGIETTERNYRYAGWKKAVKRAMAWEEHDE"
     gene            17652..18662
                     /gene="glpX"
                     /locus_tag="ACHKBL_002378"
     CDS             17652..18662
                     /gene="glpX"
                     /locus_tag="ACHKBL_002378"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HFO5819239.1"
                     /translation="MRRELAIEFSRVTESAALAGYKWLGRGDKNTADGAAVNAMRIML
                     NQVNIDGTIVIGEGEIDEAPMLYIGEKVGTGRGDAVDIAVDPIEGTRMTAMGQANALA
                     VLAVGDKGCFLNAPDMYMEKLIVGPGAKGTIDLNLPLADNLRNVAAALGKPLSELTVT
                     ILAKPRHDAVIAEMQQLGVRVFAIPDGDVAASILTCMPDSEVDVLYGIGGAPEGVVSA
                     AVIRALDGDMNGRLLARHDVKGDNEENRRIGEQELARCKAMGIEAGKVLRLGDMARSD
                     NVIFSATGITKGDLLEGISRKGNIATTETLLIRGKSRTIRRIQSIHYLDRKDPEMQVH
                     IL"
     gene            18759..19505
                     /gene="fpr"
                     /locus_tag="ACHKBL_002379"
     CDS             18759..19505
                     /gene="fpr"
                     /locus_tag="ACHKBL_002379"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004324 - ferredoxin-NADP+ reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP(+) reductase"
                     /protein_id="HFO5819240.1"
                     /translation="MADWVTGKVTKVQNWTDALFSLTVHAPVLPFTAGQFTKLGLEIE
                     GERVQRAYSYVNSPDNPDLEFYLVTVPDGKLSPRLAALKPGDEVQVVSEAAGFFVLDE
                     VPDCETLWMLATGTAIGPYLSILQLGKDLERFKNMVLVHAARYAADLSYLPLMQELEK
                     RYEGKLRIQTVVSRETAAGSLTGRLPALIESGELESAVGLPMNKETSHVMLCGNPQMV
                     RDTQQLLKETRQMTKHLRRRPGHMTAEHYW"
     gene            complement(19510..19938)
                     /gene="uspD"
                     /locus_tag="ACHKBL_002380"
     CDS             complement(19510..19938)
                     /gene="uspD"
                     /locus_tag="ACHKBL_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspD"
                     /protein_id="HFO5819241.1"
                     /translation="MAYKHIGVAISGNEEDALLVNKALELARHNDAHLTLIHIDDGLS
                     ELYPGIYFPATEDILQLLKNKSDNKLYKLTKNIQWPKTKLRIERGEMPETLLEIMQKE
                     QCDLLVCGHHHSFINRLMPAYRGMINKLSADLLIVPFIDK"
     gene            complement(19965..20264)
                     /locus_tag="ACHKBL_002381"
     CDS             complement(19965..20264)
                     /locus_tag="ACHKBL_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 domain-containing protein"
                     /protein_id="HFO5819242.1"
                     /translation="MKDVVDKCSTKGCAIDIGTVIDNDNCTSKFSRFFATREEAESFM
                     TKLKELAAAASSADEGASVAYKIKDLEGQVELDAAFTFSCQAEMIIFELSLRSLA"
BASE COUNT         4986 a         5347 c         5389 g         4768 t
ORIGIN      
        1 ttatccattc tattaatacc ttgcattgga aacgctcagg aaattaaaat tgatgaaagc
       61 tggttacatc aaagcttgaa tgtcattggt cgcacagact ctcgctttgg cccaagactg
      121 actaacgacc tctaccctga atatactgta gcaggaagaa aagactggtt tgatttttat
      181 ggttatgttg atctaccaaa attctttggc gtcggcagtc actatgatgt tgggatctgg
      241 gatgagggct caccactatt tacggaaata gaacctcggt tttccattga caaattgacc
      301 ggattaaatc ttgcgttcgg cccttttaaa gaatggttca ttgcaaacaa ctatgtctat
      361 gatatgggtg acaaccagtc atcccggcaa agtacatggt atatggggct tggtacagat
      421 atcgacacgg gtctaccaat taagctttct gccaatatat acgccaagta tcagtggcaa
      481 aactatggtg ccgctaatga aaatgaatgg gacggatatc gattcaaaat aaaatatagc
      541 attcctctta cccatttatt cggagggcga ttggtataca atagttttac taactttgat
      601 tttggctccg atcttgcgga caaatcacac aataataaac gaaccagtaa tgctattgct
      661 tcaagccata tcctttccct gctatatgaa cactggaaat ttgcatttac actacgttat
      721 tttcacaacg gtggacaatg gaatgctggg gagaaagtta acttcggaga tggtccattt
      781 gaattaaaaa acacaggatg gggaacctat actactattg gttatcaatt ttaaatcagg
      841 tgatgtttta gatattttta cttaatgcca ttttgtaatt gtcataaata acttcaccat
      901 tcacactgtg aattcatcgc tcaacacaaa agccccctta ttaccggggc actgaccaaa
      961 ggcaaaatga caatcaatca ccacctccgc aggaggtggt ttagaggtga caacaaaagc
     1021 tggcaactgc cagcttttgt tcaataatca ctacaacaac tgcgccagac ggttaatatc
     1081 cgactgaatc gccccggcgg tgacgtcgcg ccctgcacct ggcccgcgga tcaccaacgg
     1141 attatcgcga taccagcggc tttcgatggc aaagacgtta tcgcacggta gtagtgatgc
     1201 cagcggatgg tcttctcgca ccgcttccac gcctactcgc gctttgccgt tggcatcgaa
     1261 acgcgccacg tagcgcaaca ccagccccat ttcacgggcc gcttccaacc gttgtaccat
     1321 ctgctcatta agttcatcgc cattttcaaa gaaatggtcg atgcttccac cttcgcaatg
     1381 ggttggcacc agtgattcaa cacgcacctg gtccggttca atgttgtaac ccgcttcacg
     1441 cgccagaatc accagcttgc gcatcacatc tttgccggag aggtcatcac gcgggtcagg
     1501 ttcagttaag ccctgctgcc acgcctgatc caccagctca gtaaacggca cgctaccgtc
     1561 gaattgcagg aacagccagg agagcgtgcc ggagaagatc ccgctgatcg acaaaatagt
     1621 gtcgccgctg tcgatcagat cgcgcacggt atggttgatc ggcaaacctg cgccgacggt
     1681 ggcgttgtac agccagtgac gcccggtttt ttcgaacgcg tcgtggatct ggcgatattt
     1741 attgctgtcg ctcgctcccg ccagtttgtt ggcactgatg acgtggaaac cgtggctggc
     1801 gaaatcgaga tactgatcag caagctgctg gctggcagta acgtccagca caactaaatc
     1861 atcatacgga tgggcacgca tccacaggaa tagcgactct tcatcctgtt cgacggcttc
     1921 atcattaaag aaggctagcg cacggctggc gtccagcccg tcatagctca acaggctgcg
     1981 acggctgtcc accacgcctg ccagcacaaa ttcaaagccg gtgcgcgccg agagcgtgct
     2041 ctgctcacgg gcgaacagtt ccagccagcg ggaaccgata tttcccttac cgaacaatac
     2101 caggccgatg cgtttttctg cgcggaagac ggactgatgc agcccctgaa tcaggctttc
     2161 ggtcgggccg gtgcgcagta ctgccaccag gctgatgccg tcatcggact gccaggtaaa
     2221 ctcgaccggc tggcctttca gttgctgcca gaagcggtgg caatgcagcg ggttacgggt
     2281 gacgcccgcc ccaaccatcg ccaccagcgc cagcccctga cgcagacgca gttcgccggg
     2341 taatcctgct tcgtcgagga ttttcagcgc actgtcggcc acttccgagg tgtagcaaaa
     2401 ttgcagcaac tggcgatcgt tatgcacgcc aactgccagt gggcgcacct gagcacgttt
     2461 caggatttgg tcgatctctt tatgcgccag tttaaaatcc tgactggcgg gcacctgaaa
     2521 ctcaatcaaa cagacatcat cgtggctggt gacaatacgc gcaccagtac cggaggccag
     2581 cacgcgttca atgcgcgtgg aaccttgatc cggcgtgtag ctacagcgca gctgcaggtc
     2641 gatttcacta ccggatactg gctgtaaagt acgggcgtga agaacggggg ccgccagacg
     2701 cgccagttcg ctggcttcat caagacgcag caatggcagc agacaggcat ctttcacttt
     2761 acgcgggtcg gcactatata ccccggcgac atcgctccag atggttacgc gggaaacacc
     2821 cgccagcgca ccgatttgtg tcgcggaata gtcggaaccg ttacgcccca gcagcaccgt
     2881 ttcaccggcg ttgttacggc tgataaaccc ggtcaccacc agacgtttgc ccggatgttg
     2941 taccagtagc tgttgcagca acgggtaaga aagcccttca tcaacctgcg gctgcgcggc
     3001 gcgttcagcg cgtaaaaact cgcgggcatc aagccaggcc gctggcagcc cttgttgatt
     3061 aagtaccgca gacatcaggc gcgccgacca cacttcaccg tggcccacca cttccgcata
     3121 caccgcatca ttaataccgc tgtcgagcag cgccgcgagg cgttccagat cgctgacaaa
     3181 ggcgctaatg aggctatcgg cttcttcggc gggtagcagg ccgctaatca gatcgcactg
     3241 ataacgacgt agcgtttgtt gaacctgatg cgcagagaga cgatcggtct ggcttagttt
     3301 caaccagtta atcaactggt tagtggtgct accggcggcg gaaaccacca tcatatcgtc
     3361 aggctgagag tactccgcca taatgcccgc gacacgcaaa taacacttca catcagccag
     3421 actactgcca ccaaatttat gcagttgacg acctttcgcc cctgcctgcg caatcacact
     3481 catttttacc ccttgtttgc agcccggaag ccattttcca ggtcggcaat taaatcttcg
     3541 ccatcttcaa taccggtgga gatacgtagc agcgtctcgg agatcccggc ggcagcacgc
     3601 gcttctggcg ccatgcctgc atgtgtcatg gttgcggcgt gagagattaa actttccact
     3661 ccccctaatg attccgccag cgtaaacaac gacaacccgc ccaggaaacg acgcagcgtc
     3721 tgctcatcgc catccagttc aaaactcaac attgcgccaa agcctttttg ctggcgcgcg
     3781 gcaatttcat gcccctgatt ttccggcagc gacgggtgat acagtttttt caccaacggc
     3841 tgggtttgca gatatttcac aatcgcctgc gcgttgcgct gcgccagctc catacgcggc
     3901 accagcgttc gcaacccgcg cagcagcaga tagctgtcaa acgcgccgcc cgtcacgcca
     3961 atattgtttg cccaccaggc aagttcagtg acaacgtccg ggtctttagc aatcactacg
     4021 ccggccacca cgtctgagtg accgttcaga tatttcgtac atgaatgcaa caccagatcg
     4081 gcacctaatg ccagcggatt ttgtaatgcc gggcttaaga aggtgttatc caccacgctc
     4141 accgccccga cttcccttgc cagatggcag attttcgcaa tatccacgac gcgtaacaat
     4201 ggattacttg ggctttctac cagtaccagt ttgggttttt ctgccagcgc tacccgtaat
     4261 gcctgttcat cgccttgatc aacaaacaac acgcgatagc aaccgcgttt cgccagactg
     4321 tcgaacaggc gatagctgcc gccatagcag tcgtgcggcg caaccagcag atcgcccggt
     4381 ttcaaaaaga cggtcgttac cagatgaatc gcggacatgc cggtattagt aagtacggca
     4441 ccagcaccgc cttccagttc tgccagcgca cgctgaacca catcgcgcgt tgggttgccg
     4501 cgccgcgagt aatcatgcgc gcgcggttca ttaaatccgg taaagttata ggtgctggaa
     4561 agatggatcg gtgggacaac gcaaccatac tgttcgtcgt catttaaccc gctacgcact
     4621 gcgatggtgg cctgtttacg cgtcatgtga tgaagttccc tgggcttggt cggtgaaatg
     4681 tcaggcacca gagtaaacat tgtgttaatg gacgtcaata catctggaca tctaaacttc
     4741 tttgcgtata gattgagcaa atcccaaata gccgttaaaa ttatatgcat tatcacgccg
     4801 acaggtgcat tacacgatgt cacggtagcg cctgtacggt aaactatgcg ggtttacggt
     4861 cagtacccac ataaattgtg tggtctggtc tcaatttatt gacgaagagg attaagtatc
     4921 tcatggctga atggagcggc gaatatatca gcccatacgc tgagcacggc aagaagagtg
     4981 aacaagtcaa aaagattacg gtttccattc ctcttaaggt gttaaaaatc ctcaccgatg
     5041 aacgcacgcg tcgtcaggtg aacaacctgc gtcacgctac caacagcgag ctgctgtgcg
     5101 aagcgtttct gcatgccttt accgggcaac ctttgccgga tgatgccgac ctgcgtaaag
     5161 agcgcagcga cgaaatcccg gaagcggcaa aagagatcat gcgtgagatg gggattaacc
     5221 cggagacgtg ggaatactaa agcggaaaag agcggcgcgg agttaaccag ccgctttttt
     5281 catacatgga cgtttaacga tgaaaatcag gctactgatc ctcagcctgc tggtttctgt
     5341 gcctgccttt gcctggcagc cgcaaactgg cgacatcatc tttcaaatct ctcgctcatc
     5401 gcaaagtaaa gcgatccaac tggcgaccca ttccgattac agccacaccg gtatgctggt
     5461 gatacgcaac aaaaagccct acgtttttga agcggtcggc ccggtaaaat acaccccact
     5521 caagcagtgg atcgcccatg gtgaaaaggg caaatacgtt gttcgccgcg ttgaaggcga
     5581 actgagtgtt gaacaacagc aaaaactggc gcaaacggca aaacgttatc tcggtaaacc
     5641 gtatgactcc agcttctcgt ggagcgacga tcgccagtac tgttcggaag tggtttggaa
     5701 ggtttatcag aacgcgctgg gaatgcgcgt gggcaaacag caaaagttga aagagttcga
     5761 ccttagtaac ccgcaggttc aggcaaagct caaagaacgc tacggcaaaa atattccgct
     5821 tgaggaaacg gtggtctcgc cacaagccgt tttcgatgcg ccacaactca ccacggtcgc
     5881 caaagaatgg ccgctgtttt cgtggtaata cgaccaaaat tcaggcacaa aaaaagcgcc
     5941 gtgcggcgct tttttcggaa atccggtctt atttgctgcc cgggatgttg aaacgcttgt
     6001 tgaagcggtc aacacggcca ccggtagcaa catcacgctg tttgccagtg aagaacgggt
     6061 ggcacttgct gcacacgtcg aggttcaggt catgaccaac ggtggagcgg attttcatta
     6121 cgttaccgca agagcagcta gcagtaattt cttcgtattt cgggtgaata tcttttttca
     6181 tgggaaaacc tcggtttaag gccgcgtcgc tcttccagcc ctaacgccag acaccacgcg
     6241 atgttaaaag tatagcttca atacgatcat ttcgtatgaa ggcgcgaaat catacagaaa
     6301 ttaaccagcg tatgcaaact gatccgcact cttctacggc aatgtgtata ctaacccacc
     6361 gaatttcaag tcaggatgat gctatgcccg ttgcccacgt tgccttgccc gttccgcttc
     6421 cccgtacctt tgactatctg ctgccagaag gcatgacggt caaagccggg tgtcgcgtgc
     6481 gcgtgccgtt tggcaaacag caggagcgca tcggggttgt ggtgtcagtt agcaatgtca
     6541 gcgagctgcc gcttaacgag ctgaaagcgg tagttgaagt gctggacgtt gagccggtat
     6601 ttactcactc cgtctggcga ttactgctct gggcgacaga ttactatcat catccgattg
     6661 gcgatgtgct gtttcatgcc ttgccgattt tactacgcca ggggcggcct gcggcgaacg
     6721 cgccgatgtg gtactggttt gccactgaac aaggtcaggc ggtggatctg aacagcctga
     6781 aacgctcacc caagcaacaa caggcgctgg cagcgttacg gcaaggcaaa atctggcgcg
     6841 accaggttgc cacgctcgaa tttaatgatg ccgcgttgca ggcgctgcgc aaaaaaggtc
     6901 tgtgtgattt agcaagtgaa acaccagagt ttagtgactg gcgaaagaat tatgccgttt
     6961 ctggtgagcg gttgcgattg aatactgaac aggccaccgc cgttggcgca attcatagtg
     7021 cggcggatac cttctctgcc tggctgctgg cgggcgttac cggttccggt aaaacggagg
     7081 tttatctcag tgtactggaa aacgtgctcg ctcagggcaa acaggcgctg gtgatggtgc
     7141 cggaaatcgg cctgacgccg caaactatcg cccgttttcg tgaacgtttt aatgcgccag
     7201 tggaagttct gcattccggc ctgaacgaca gcgagcgtct ttcggcgtgg ttgaaagcga
     7261 aaaatggtga ggcggcgatt gtgatcggca cccgctccgc gctgtttacg ccgtttaaaa
     7321 atctcggtgt gattgtcatt gatgaagagc acgacagttc ctacaagcag caggaaggct
     7381 ggcgctatca tgcccgcgac ctggcggttt atcgtgcgca cagcgagcaa atcccgatta
     7441 ttcttggctc cgcaacaccc gcactggaaa cgttatgcaa cgtccagcag aaaaaatacc
     7501 gcctgctgcg cctgacgcgt cgggcgggga atgcgcgtcc ggcaattcaa cacgtgctgg
     7561 atttaaaagg tcagaaggtg caggcggggc tggctccggc gttaatcacc cgtatgcgcc
     7621 agcatttaca ggccgacaac caggttatcc tctttcttaa ccgccgtggc tttgcgcctg
     7681 ccttgctgtg ccacgactgc ggctggattg ccgaatgtcc acgttgcgat cactactaca
     7741 cgctgcatca ggcgcaacag catttgcgct gtcaccactg tgacagccaa cgcccggtgc
     7801 cgcgccagtg cccttcctgc ggttccacgc acctggtgcc ggtggggctg ggcaccgaac
     7861 agcttgaaca gacgcttgcg ccgctgttcc ctggtgtgcc catttctcgt atcgaccgcg
     7921 ataccaccag ccgcaaagga gcgctggaac aacaactggc ggaagtgcat cgcggcggcg
     7981 cgaggatctt gatcggcacg caaatgctgg cgaaaggtca ccactttccg gatgtgacgc
     8041 tggtggcgtt actggacgta gacggcgcgc tgttttctgc cgattttcgc tcggcagagc
     8101 gtttcgccca gctttacacc caggtcgccg gtcgtgccgg gcgtgcgggt aaacagggcg
     8161 aagtggtgct gcaaacgcat catccagaac acccgctatt gcaaacgttg ctctataaag
     8221 gctatgacgc ctttgccgaa caggcactgg ctgagcgacg catgatgcag ctgccgccgt
     8281 ggaccagcca tgtgattgtg cgtgcggaag atcataacaa tcagcacgcg ccgctgttcc
     8341 tgcaacaact gcgtaatctc atcctctcca gcccgctggc agacgacaaa ctgtgggttc
     8401 tcggtcccgt tccggcactg gcacctaaac gtggcggccg ctggcgctgg caaattttgt
     8461 tgcagcaccc ttcccgcgtg cgattacagc acatcatcaa tggtacgctg gcgcttatta
     8521 atacaatacc ggattcccgt aaggtgaaat gggtgctgga tgtcgatccc atcgagggct
     8581 aatgcgctca cgatgcgagg cggatcgaaa aattcaatat tcatcacact tttcatgaaa
     8641 attctgtaac cgttttcacg cgctatctgc taaaaatgtt gccgatgtga agtaaacatg
     8701 gatgtagtac gcctgacgtg ccaggcgagg agtgagtgtg aaagcgaaga agcaggaaac
     8761 tgccgcgacc atgaaagacg ttgccctcaa ggcaaaagtc tctacagcga ctgtctcccg
     8821 agcattaatg aatcccgata aagtctccca ggccacccgt aatcgggttg aaaaagcggc
     8881 ccgggaagtg ggttatttac cgcagcctat ggggcgcaac gtcaagcgta atgaatcccg
     8941 cactattctg gtgattgtcc cggatatctg cgatcccttc tttagcgaaa ttattcgcgg
     9001 tatcgaagtt acggcggcaa atcacggata tctggtgctg attggcgact gtgcgcatca
     9061 aaatcagcag gaaaaaacct ttatcgattt gatcatcacc aagcaaattg atggcatgtt
     9121 gctgctgggt tcaaggctgc cgtttgatgc cagcattgag gaacagcgta atctgccgcc
     9181 gatggtgatg gcgaacgaat ttgcaccgga actggagctg cctaccgttc atatcgacaa
     9241 tctgaccgcc gcatttgatg cagtaaatta tttatatgag caagggcata atcggattgg
     9301 ctgtatagcc ggtcccgaag agatgccgct gtgtcactac cgcctgcaag gctatgtcca
     9361 ggcgctgcgt cgctgcggca ttatggttga tccgcaatac atcgcccgtg gcgacttcac
     9421 cttcgaagcc ggaagcaaag cgatgcagca gttgcttgat cttccacaac cgcctactgc
     9481 tgtcttctgc catagcgatg tgatggcgct cggcgcactt tctcaggcaa aacgccaggg
     9541 gctgaaagtc ccggaagacc tttccataat cggttttgat aacatcgacc tgacgcaatt
     9601 ttgtgatccg ccgctgacaa ccatcgcgca gccgcgttac gaaatcggtc gggaagctat
     9661 gctgttattg cttgatcaaa tgcaggggca acacgttggc agtggctctc gtttaatgga
     9721 ctgcgaactt atcatccggg gatcaacacg cgcgttacct taaagtaaac ctttaagact
     9781 gccgtgactg gtcaaaggcc cgccgcttaa gtaacatggc gggctgacga acgaataaat
     9841 acagcgaaac gatagtggca caacgagatt atgtacgccg cagccaaccg gcaccttcgc
     9901 ggcgaaaaaa gagcacctca cggaaaaagc aacgaaatct gcctgcggtt tctcccgcta
     9961 tggtcgctat tgctgccgcc gttcttgtga cctttatcgg tggtctgtac ttcattacgc
    10021 atcacaagaa agaagagtcc gagacactgc aaagccagaa agtgaccgga aacggactac
    10081 caccaaaacc agaagaacgc tggcgctaca tcaaagagct ggaaagtcgc cagccgggtg
    10141 tgcgtgcgcc tacagaacct tctgccggtg gtgaagtgaa aacgccggag caactgacac
    10201 cagaacaacg ccagcttctt gagcaaatgc aggctgatat gcgccagcag ccaacgcagc
    10261 tggttgaagt tccgtggaat gaacagacgc cggaacagcg tcagcaaacg ctacagcgcc
    10321 agcgtcaggc gcagcaatta gcggagcagc aacgcctggc tcagcagtcg cgtacgactg
    10381 aacaaagctg gcagcagcag acgcgtacgt cgcaagccgc gccggtacag gcgcaaccgc
    10441 gccagtctaa accggcttct actcagcagc cgtaccagga tctgctgcaa acccctgcgc
    10501 acacgaccgc gcagtcgaag ccgcagcaag ctgcgccggt tgctcgtgcc gctgacgcgc
    10561 caaaaccgac ggcggagaaa aaagacgaac gccgctggat ggtgcagtgc ggttcgttca
    10621 gaggcgcgga acaggcagag acggtacgtg ctcagctggc gttcgaaggc tttgactcaa
    10681 aaattaccac caacaatggc tggaatcgtg tggtgattgg cccggtgaaa ggcaaagaga
    10741 acgcagacag caccctcaat cgcttgaaga tagcgggtca tacaaactgc attcggctcg
    10801 ccgccggggg ttgaaaccct caaaatcccc cccatctata attgcattat gccccgtact
    10861 tttgtacggg gtttgtactc tgtattcgta accaaggggt cagctcgtga caactatagt
    10921 aagcgtacgc cgtaacggcc atgtggtcat cgctggtgat ggtcaggcca cgttgggcaa
    10981 taccgtaatg aaaggcaacg tgaaaaaggt ccgccgtctg tacaacgaca aagtcatcgc
    11041 gggctttgcg ggcggtactg cggatgcttt tacgctgttc gaactgtttg aacgtaaact
    11101 ggaaatgcat cagggccatc tggtcaaagc cgccgttgag ctggcgaaag actggcgtac
    11161 cgatcgcatg ctgcgcaaac ttgaagcatt gctggcagtc gcggatgaaa ctgcatcgct
    11221 tatcatcacc ggtaacggtg acgtggtgca gccagaaaac gatcttattg ctatcggctc
    11281 cggcggccct tacgcccagg ctgcggcgcg cgcgctgtta gaaaacactg aacttagcgc
    11341 ccgtgaaatt gctgaaaagg cgttggatat tgcaggcgac atttgcatct ataccaacca
    11401 tttccacacc atcgaagaat taagctacaa agcgtaagga tctcccatgt ctgaaatgac
    11461 cccacgcgaa atcgtcagcg aactggataa gcacatcatc ggccaggaca acgccaagcg
    11521 ttccgtggcg attgctctgc gtaaccgctg gcgtcgcatg cagctcaacg aagagctgcg
    11581 ccatgaagtg accccgaaaa atatcctgat gatcggcccg accggtgtcg gtaaaactga
    11641 aatcgcccgt cgcctggcta agctggcgaa tgcgccgttc atcaaagttg aagcgaccaa
    11701 attcaccgaa gtgggctatg tcggtaagga agtggattct attattcgcg atctgaccga
    11761 tgccgccgtg aaaatggtac gcgtccaggc tatcgagaaa aaccgttatc gcgctgaaga
    11821 actggcagaa gaacgtattc tcgacgtact gatcccaccg gctaaaaata actggggaca
    11881 gaccgaacag cagcaggaac cgtccgctgc tcgtcaggca ttccgcaaaa aactgcgtga
    11941 aggccagctt gatgacaaag aaatcgagat cgatcttgcc gcagcaccga tgggcgttga
    12001 aattatggct cctccgggca tggaagagat gaccagccag ctgcagtcca tgttccagaa
    12061 cctgggcggt cagaaacaaa aagcgcgtaa gctgaaaatc aaagacgcca tgaagctgct
    12121 gattgaagaa gaagcggcga aactggtgaa cccggaagag ctgaagcaag atgctatcga
    12181 cgctgttgag cagcacggga tcgtgtttat cgacgaaatc gacaaaatct gtaagcgcgg
    12241 cgagtcttcc ggtccggatg tttctcgtga aggcgttcag cgtgacctgc tgccgctggt
    12301 agaaggttgc actgtttcca ccaaacatgg gatggtcaaa actgaccaca ttctgtttat
    12361 cgcttctggc gcgttccaga ttgcgaaacc gtctgacctg atcccggaac tgcaaggtcg
    12421 tctgccaatc cgcgttgagc tgcaggcact gaccaccagc gacttcgagc gtattctgac
    12481 cgagccgaat gcctctatca ccgtgcagta caaagcactg atggcaactg aaggcgtaaa
    12541 tatcgagttt accgactccg gtattaagcg catcgcggaa gcggcatggc aggtgaacga
    12601 atctaccgaa aacatcggtg ctcgtcgttt acataccgtt ctggagcgtt taatggaaga
    12661 gatttcctac gacgccagcg atttaagcgg tcaaactatc actattgacg cagattatgt
    12721 gagcaaacat ctggatgcgt tggtggcaga tgaagatctg agccgtttta tcctataatc
    12781 gcgttcaatc attttcatca ttgtttgatg gggctgaaag gccccatttt tattggcgcg
    12841 tattatgact gaacaacaaa ttagccgaac tcaggcgtgg ctggaaagtt tacgacctaa
    12901 aaccctcccc ctcgcctttg ctgcaattat cgtcgggacg gcgctggcat ggtggcaagg
    12961 tcacttcgat ccactggtcg ccctgctggc attgattacc gccgggctat tacagatcct
    13021 ttctaacctc gccaatgatt acggcgatgc ggtaaaaggc agcgataaac ctgaccgcat
    13081 tgggccgcta cgcggcatgc aaaaaggggt cattacccag caagagatga aacgggcgct
    13141 cattattacc gttgtgctca tctgtctttc cgggctggca ctggttgcag tggcgtgcca
    13201 tacgctggcc gattttgtcg gtttcctgat tcttggcggg ttgtcgatca ttgccgctat
    13261 cacctacacc gtgggcaatc gtccttatgg ttatatcggt ttgggtgata tttccgtact
    13321 ggttttcttt ggctggttga gcgtcatggg gagctggtat ttacaggctc atacattgat
    13381 tccggcactg atccttccgg caaccgcatg cggcctgctg gcaacggcag tactgaacat
    13441 taataacctg cgtgatatca atagcgaccg cgaaaacggc aaaaacacgc tggtggtgcg
    13501 cttaggtgca gtaaacgcgc gtcgttatca tgcctgcctg ctgatgggct cactggtgtg
    13561 tctggcgctg tttaatctct tttcgctgca tagcctgtgg ggctggctgt tcctgctggc
    13621 ggcaccatta ctggtgaagc aagcccgtta tgtgatgcgg gaaatggacc cggtggcgat
    13681 gcgaccgatg ctggaacgca ctgtcaaggg agcgttactg actaacctgc tgtttgtttt
    13741 agggatattc ctaagccagt gggcagcata actgacaaat atcaattaac aattgatgat
    13801 tttgccaaca gcctacatag cgcgatatac tgaaaattct cgcagcaact gaatgttaag
    13861 cctatgaaat acgatacttc cgagctttgt gacatctatc aagaagatgt taacgtcgtg
    13921 gaaccgctgt tctccaactt tggcggacgg gcgtcgtttg gcggacaaat aatcacggta
    13981 aaatgtttcg aggacaacgg gttgctgtac gatctgctcg aacagaatgg ccgtggtcgt
    14041 gttcttgtcg tcgatggcgg tggttctgtt cgtcgcgcac tggtcgatgc tgaactggcg
    14101 cgtctggcag tacaaaatga atgggaaggt ctggtcattt acggcgcggt gcgtcaggta
    14161 gatgacctgg aagagctgga tatcggcatc caggcaatgg ccgcaattcc ggttggtgcc
    14221 gctggcgaag gcattggcga aagcgatgtc cgcgtcaatt ttggcggtgt aaccttcttc
    14281 tccggcgacc atctttacgc cgacaatacc gggattattc tttcagaaga tccgctggat
    14341 attgaatgat aaaaaaggca ccgcaaggtg ccttttttct gcgttacctg ttggcctaca
    14401 cagtaaagaa attacgcgaa agatgaagcg taatcagacc tcttccatgc gacccagcag
    14461 ggcctgcaga cgttcctgcc agccgttctg ctgttctttc agatggttgt tctcacgctc
    14521 cagctcttcg cgctgatgct gggcattttg aacttcctgc gacagtgagt tgtttttttc
    14581 tttcagctct tcgatttcca tctgcaacag agtgatggta tcaatcgcct gctgtacttt
    14641 tgcttccagt ttctcaaaca cttctaatga cattgtcata cctctcctga attgcaaggc
    14701 gttgatggat aaaaaatcct cgtcccgatt accggtgacg ccttaataaa tacgagcgca
    14761 ctttagttag ctccgattgt atgaagccgc gccatcgctg tccagcggca cgccttgcag
    14821 attacggttt gccacacttt tcatccttct cctggagaca taatccacac caatcgaaaa
    14881 tgttaataaa tttgttgcgc gaatgatcta acaaacatgc atcatgtaca atcagatgga
    14941 ataaatggcg cgataacgct cattttatga cgaagcacac acattttaag ttcgatattt
    15001 ctcgtttttg ctcgttaacg ataagtttac agcatgccta caagcatcgt ggaggtccgt
    15061 gactttcacg catacaacaa acattaactc ttcaggatcc gattatgagt caaacatcaa
    15121 ccttgaaagg ccagtgcatt gctgaattcc tcggtaccgg gttgttgatt tttttcggtg
    15181 tgggttgcgt tgcagcacta aaagtcgctg gtgcgtcttt tggtcagtgg gaaatcagtg
    15241 tcatttgggg actgggggtg gcaatggcca tctacctgac cgcaggggtt tccggcgcgc
    15301 atcttaaccc cgctgttacc attgcattgt ggctgtttgc ctgtttcgac aagcgcaaag
    15361 ttatcccttt tatcgtttca caagttgccg gcgctttctg tgctgcggct ttagtttacg
    15421 ggctttacta caatttattt ttcgacttcg agcagactca tcatattgtt cgcggcagtg
    15481 ttgaaagtgt tgatctggct ggcactttct ctacttaccc taatcctcat atcaattttg
    15541 tgcaggcttt cgcagttgag atggtgatta ccgctattct gatggggctg atcctggcgt
    15601 taacggacga tggcaacggt gtaccacgcg gccctttggc tcccttgctg attggtctac
    15661 tgattgcggt cattggcgca tctatgggcc cattgacggg ttttgccatg aacccagcgc
    15721 gtgacttcgg tccgaaagtc tttgcctggc tggcgggctg gggcaatgtc gcctttaccg
    15781 gcggcagaga cattccttac ttcctggtgc cgctttttgg ccctatcgtt ggcgcgattg
    15841 taggtgcatt tgcctaccgc aaactgattg gtcgccattt gccttgcgat atctgtgttg
    15901 tggaagaaaa ggagaccaca actccttcag aacaaaaagc ttcgctgtaa tatgactacg
    15961 ggacaattaa acatgactga aaaaaaatat atcgttgcgc tcgaccaggg caccaccagc
    16021 tcccgcgcgg tcgtaatgga tcacgatgcc aatatcatta gcgtgtcgca gcgcgaattt
    16081 gagcaaatct acccaaaacc aggctgggta gaacacgacc caatggaaat ctgggccacc
    16141 caaagctcca cgctggtaga agtgctggcg aaagccgata tcagttccga tcaaattgca
    16201 gctatcggta ttacgaacca gcgtgaaacc actattgtct gggaaaaaga aaccggcaag
    16261 cctatctata acgccattgt ctggcagtgc cgtcgtaccg cagaaatctg cgagcattta
    16321 aaacgtgacg gtttagaaga ttatatccgc agcaataccg gtctggtgat tgacccgtac
    16381 ttctctggca ccaaagtgaa gtggatcctc gaccatgtgg aaggctctcg cgagcgtgcg
    16441 cgtcgtggtg aattgctgtt tggtacggtt gatacgtggc ttatctggaa aatgactcag
    16501 ggccgtgtcc atgtgaccga ttacaccaac gcctctcgta ccatgttgtt caacatccat
    16561 accctggact gggacgacaa aatgctggaa gtgctggata ttccgcgcga gatgctgcca
    16621 gaagtgcgtc gttcttccga agtgtacggt cagactaaca ttggcggcaa aggcggcacg
    16681 cgtattccaa tctccgggat cgccggtgac cagcaggcgg cgctgtttgg tcagttgtgc
    16741 gtgaaagaag gaatggcgaa gaacacctat ggcactggct gctttatgct gatgaacact
    16801 ggcgagaaag cggtgaaatc agaaaacggc ctgctgacca ccatcgcctg cggcccgact
    16861 ggcgaagtaa actatgcgct ggaaggtgcg gtgtttatgg cgggcgcgtc cattcagtgg
    16921 ctgcgcgatg agatgaagtt gattaacgac gcctacgatt ccgaatattt cgccaccaaa
    16981 gtgcaaaaca ccaatggtgt gtatgtggtt ccggccttta ccgggctggg tgcgccatac
    17041 tgggatccgt atgcacgcgg ggcgattttc ggtctgactc gtggggtgaa cgctaaccac
    17101 attatacgcg cgacgctgga gtctattgct tatcagacgc gtgacgtgct ggaagcaatg
    17161 caggccgact ccggtattcg tctgcacgcg ctgcgcgtgg acggcggtgc ggtggcgaac
    17221 aatttcctga tgcagttcca gtccgatatt ctcggtaccc gcgttgagcg cccggaagtg
    17281 cgcgaagtca ccgctttggg tgcggcctat cttgctggtc tggcggttgg cttctggcag
    17341 aatcttgacg agctgcaaga gaaagcggtg attgaacgcg agttccgtcc aggcatcgaa
    17401 accactgagc gtaattaccg ttacgcaggc tggaaaaaag cggtcaaacg cgcgatggca
    17461 tgggaagaac acgacgagta ataatgattg cctgatgcga cgcttgcgca tcttatcagg
    17521 cctacgagtc ggatacacat ccggcatcac gcagactctg aaaccgtgcg ggggcaaccc
    17581 cgcacacatc aataatcccg cccttcccct gtgctacact tcgcgccatt ccttactgct
    17641 tagagtttgc tatgagacga gaacttgcca tcgaattttc ccgcgtcacc gaatcagcgg
    17701 cgctggctgg ctacaaatgg ttaggacgcg gcgataaaaa caccgcggac ggcgcggcgg
    17761 taaacgccat gcgtattatg ctcaaccagg tcaacattga cggcaccatc gtcattggtg
    17821 aaggtgaaat cgacgaagca ccgatgctct acattggtga aaaagtcggt actggtcgcg
    17881 gcgacgcggt agatattgct gttgatccga ttgaaggcac gcgcatgacg gcgatgggcc
    17941 aggctaacgc gctggcggtg ctggcagtag gcgataaagg ctgtttcctc aacgcgccgg
    18001 atatgtatat ggagaagctg atcgtcggac cgggagccaa aggcaccatt gatctgaacc
    18061 tgccgctggc ggataacctg cgcaatgttg cggcggcgct cggcaaaccg ttgagcgaac
    18121 tgacggtaac gattctggca aaaccgcgcc acgatgccgt tatcgcagaa atgcagcaac
    18181 tcggcgtgcg cgtatttgct attccggacg gcgacgttgc agcctcaatt ctcacctgta
    18241 tgccagacag cgaagttgac gtgctgtacg gtattggtgg cgcgccggaa ggcgtggttt
    18301 ctgcggcggt gatccgcgca ttagatggtg acatgaacgg tcgtctgctg gcgcgtcatg
    18361 acgtcaaagg cgacaacgaa gagaatcgtc gcattggcga gcaggagctg gcacgctgca
    18421 aagcgatggg catcgaagcc ggtaaagtat tgcgtttggg cgatatggcg cgcagcgaca
    18481 acgtcatctt ttctgccacc ggcatcacca aaggtgatct gctggaaggt attagccgca
    18541 aaggcaatat cgccaccacc gaaacgctgc tgatccgtgg taaatcacgc accattcgtc
    18601 gcattcagtc catccactat ctggatcgta aagacccgga aatgcaggtg cacatcctct
    18661 gattgatttg atcgattgag ccttccagtc cttcgggact ggaatttttt tgttcggaga
    18721 acgaagataa ggcaagtcaa tcaaaacagg agaaaaacat ggctgattgg gtaacaggca
    18781 aagtcactaa agtgcagaac tggaccgacg ccctgtttag tctcaccgtt cacgctcccg
    18841 tgcttccgtt taccgccggg caattcacca aacttggcct tgaaattgaa ggcgaacgcg
    18901 ttcagcgcgc ctactcctat gttaactcgc ccgataatcc ggatctggag ttttacctgg
    18961 tcaccgtccc cgatggcaaa ttaagtccac gactggcggc actgaaaccg ggcgatgaag
    19021 tgcaggtggt tagcgaagcc gctggcttct ttgtgctgga tgaagtacca gattgcgaaa
    19081 cgctatggat gctggcaacc ggtacagcga ttggccctta tttatcgatt ctgcaattgg
    19141 gcaaagattt agaacgcttc aaaaacatgg tgctggttca cgccgcacga tatgccgccg
    19201 acttaagcta tctacctctg atgcaggaac tggaaaaacg ctacgagggg aaactgcgca
    19261 ttcagacggt ggtcagtcgg gaaacggctg cgggttcgct cactgggcga ttaccggcgt
    19321 taattgaaag tggtgaactg gaaagcgcgg ttggcctgcc gatgaataaa gaaaccagcc
    19381 atgtgatgct gtgcggcaat ccacagatgg tgcgcgatac acaacagttg ctgaaggaga
    19441 cccggcagat gacgaaacat ttacgtcgcc gaccgggcca tatgacagcg gagcattact
    19501 ggtaagcggt tacttgtcga taaacggcac gatcaagaga tccgcactca acttattgat
    19561 catcccgcga tatgccggca tcaaacggtt gataaatgag tgatgatgac cacagacaag
    19621 gaggtcgcac tgctcttttt gcataatttc cagcagcgtt tccggcattt ctccgcgttc
    19681 aatacgcagt tttgtcttcg gccattgaat atttttcgtc agtttataca gcttgttatc
    19741 cgacttattc ttcaacaatt gaagaatatc ttctgttgca gggaagtaga tacccggata
    19801 caactcgctt aagccatcat caatatgaat taacgtcagg tgagcgtcat tatgtctggc
    19861 gagctccagg gctttattca ccagtaaggc atcttcttca ttcccggaaa ttgccacgcc
    19921 aatgtgttta taagccatgt ttaactcctt ctaaagcaac tccatcaagc taacgaacgc
    19981 agtgataact caaaaataat catctcggcc tggcatgaga aggtgaaggc cgcatcaagc
    20041 tcaacttgcc cctccagatc cttaatctta taggccacgc tggccccttc atctgcagag
    20101 gatgcagcgg cggcaagctc tttcagtttg gtcataaaag actctgcttc ttcgcgggtg
    20161 gcaaaaaagc gcgaaaactt actggtacag ttgtcgttat caatcaccgt accgatatca
    20221 atcgcacatc ctttagtact gcatttatct acgacatctt tcatagggca cctctgtata
    20281 ttcgcctctc tgtcgattac gacttaagaa taatgcgccg ttaaaggaag aacaaacaag
    20341 taatgatctt atggattacc attttttttg atattgctta aggtgaagag ttcagaagga
    20401 ttttccgggc attgtcactt gcatcgtgga aataacaatc ccggaaaaaa atagcgataa
    20461 gagatatatt aaaggttaat tacttgattt
//
LOCUS       DAZFLD010000077        20278 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.077, whole genome shotgun sequence.
ACCESSION   DAZFLD010000077 DAZFLD010000000
VERSION     DAZFLD010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20278
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.077"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(373..1569)
                     /gene="hemY"
                     /locus_tag="ACHKBL_002382"
     CDS             complement(373..1569)
                     /gene="hemY"
                     /locus_tag="ACHKBL_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0042168 - heme metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX biogenesis protein HemY"
                     /protein_id="HFO5819243.1"
                     /translation="MLKVLLLFVLLIAGIVVGPMIAGHQGYVLIQTDNYNIETSVTGL
                     AIILILAMVVLFAIEWLLRRIFRTGAHTRGWFVGRKRRRARKQTEQALLKLAEGDYQQ
                     VEKLMAKNADHAEQPVVNYLLAAEAAQQRGDEARANQHLERAAELAGNDTIPVEITRV
                     RLQLARNENHAARHGVDKLLEVTPRHPEVLRLAEQAYIRTGAWSSLLDIIPSMAKAHV
                     GDEEHRAMLEQQAWIGLMDQARADNGSEGLRNWWKNQSRKTRHQVALQVAMAEHLIEC
                     DDHDTAQQIIIDGLKRQYDDRLLLPIPRLKTNNPEQLEKVLRQQIKNVGDRPLLWSTL
                     GQSLMKHGEWQEASLAFRAALKQRPDAYDYAWLADALDRLHKPEEAAAMRRDGLMLTL
                     QNNPPQ"
     gene            complement(1572..2771)
                     /gene="hemX"
                     /locus_tag="ACHKBL_002383"
     CDS             complement(1572..2771)
                     /gene="hemX"
                     /locus_tag="ACHKBL_002383"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HFO5819244.1"
                     /translation="MTEQEKTSAVVEETREAVDTTSQPVATEKKSKNNTALILSAVAI
                     AIALAAGVGLYGWGKQQAVNQTATSDALANQLTALQKAQESQKAELEGIIKQQAVQLE
                     QANRQQETLAKQLDEVQQKVATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTAAA
                     LLKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRL
                     ADNDSDGSPMDSDGEELSSSISEWRINLQKSWQNFMDNFITIRRRDDTAVPLLAPNQD
                     IYLRENIRSRLLVAAQAVPRHQEETYRQALENVSTWVRAYYDTDDATTKAFLDEVDQL
                     SQQNISMDLPETLQSQAMLEKLMQTRVRNLLAQPAAGTTEAKPAPAPAPAPAPQADAP
                     AAAPQGE"
     gene            complement(2793..3533)
                     /gene="hemD"
                     /locus_tag="ACHKBL_002384"
     CDS             complement(2793..3533)
                     /gene="hemD"
                     /locus_tag="ACHKBL_002384"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HFO5819245.1"
                     /translation="MSILVTRPSPAGEELVSRLRTLGQVAWHFPLIEFSPGRQLPQLA
                     DQLAALGESDLLFALSQHAVAFAQSQLHQQDRKWPRLPTYFAIGRTTALALHTVSGQK
                     ILYPQDREISEVLLQLPELQNIAGKRALILRGNGGRELIGDTLTARGAEVTFCECYQR
                     CVIHYDGAEEAMRWQSREVTTVVVTSGEMLQQLWSLIPQWYREHWLLRCRLLVVSERL
                     AKLARELGWQDIKVADNADNDALLRALQ"
     gene            complement(3530..4471)
                     /gene="hemC"
                     /locus_tag="ACHKBL_002385"
     CDS             complement(3530..4471)
                     /gene="hemC"
                     /locus_tag="ACHKBL_002385"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HFO5819246.1"
                     /translation="MLDNVLRIATRQSPLALWQAHYVKDKLMASHPGLVVELVPMVTR
                     GDVILDTPLAKVGGKGLFVKELEVALLENRADIAVHSMKDVPVEFPQGLGLVTICERE
                     DPRDAFVSNNYESLDALPAGSIVGTSSLRRQCQLAERRPDLIIRSLRGNVGTRLSKLD
                     NGEYDAIILAVAGLKRLGLESRIRAALPPEISLPAVGQGAVGIECRLDDTRTRELLAA
                     LNHHETALRVTAERAMNTRLEGGCQVPIGSYAELIDGEIWLRALVGAPDGSQIIRGER
                     RGAPQDAEQMGISLAEELLNNGAREILAEVYNGDAPA"
     gene            4858..7404
                     /gene="cyaA"
                     /locus_tag="ACHKBL_002386"
     CDS             4858..7404
                     /gene="cyaA"
                     /locus_tag="ACHKBL_002386"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HFO5819247.1"
                     /translation="MYLYIETLKQRLDAINQLRVDRALAAMGPAFQQVYSLLPTLLHY
                     HHPLMPGYLDGNVPKGICLYTPDETQRHYLNELELYRGMSVQDPPKGELPITGVYTMG
                     STSSVGQSCSSDLDIWVCHQSWLDSEERQLLQRKCSLLESWAASLGVEVSFFLIDENR
                     FRHNESGSLGGEDCGSTQHILLLDEFYRTAVRLAGKRILWNMVPCDEEEHYDDYVMTL
                     YAQGVLTPNEWLDLGGLSSLSAEEYFGASLWQLYKSIDSPYKAVLKTLLLEAYSWEYP
                     NPRLLAKDIKQRLHDGEIVSFGLDPYCMMLERVTEYLTAIEDFTRLDLVRRCFYLKVC
                     EKLSRERACVGWRREVLTQLVKEWEWDDARLAMLDNRANWKIDQVREAHNELLDAMMQ
                     SYRNLIRFARRNNLSVSASPQDIGVLTRKLYAAFEALPGKVTLVNPQISPDLSEPNLT
                     FIYVPPGRANRSGWYLYNRAPNIESIISHQPLEYNRYLNKLVAWAWFNGLLTSRTRLY
                     IKGNGIVDLPKLQEMVADVSHHFPLRLPAPTPKALYSPCEIRHLAIIVNLEYDPTAAF
                     CNQVVHFDFRKLDVFSFGENQNCLVGSVDLLYRNSWNEVRTLHFNGEQSMIEALKTIL
                     GKMHQDAAPPDSVEVFCYSQHLRGLIRTRVQQLVSECIELRLSSTRQETGRFKALRVS
                     GQTWGLFFERLNVSVQKLENAIEFYGAISHNKLHGLSVQVETNHVKLPAVVDGFASEG
                     IIQFFFEETQDENGFNIYILDESNRVEVYHHCEGSKEELVRDVSRFYSSSHDRFTYGS
                     SFINFNLPQFYQIVKVDGREQVIPFRTKSIGNMPPANQDHDTPLLQQYFS"
     gene            complement(7444..7764)
                     /gene="cyaY"
                     /locus_tag="ACHKBL_002387"
     CDS             complement(7444..7764)
                     /gene="cyaY"
                     /locus_tag="ACHKBL_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HFO5819248.1"
                     /translation="MNDSEFHRLADQLWLTIEERLDDWDGDSDIDCEINGGVLTITFE
                     NGSKIIINRQEPLHQVWLATKQGGYHFDLKGDEWICDRSGETFWDLLEQAATQQAGET
                     VSFR"
     gene            complement(7812..9869)
                     /locus_tag="ACHKBL_002388"
     CDS             complement(7812..9869)
                     /locus_tag="ACHKBL_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000611185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5819249.1"
                     /translation="MINTSRQDGFMHFRNVVCLLSCSLFLSSAWGCRLSAPEHNIYQK
                     QGKGVVYLRPYKETNLSLPDVNYKSLRRLPNLLIDPTTLDKWDKVPPLTDLTTDYLYE
                     GAQAWYPHYSWHSDGRYILYAGEVVQNPPDKPPVDVASFKAWGDFAADKHSLYFEGTR
                     TDDNGGENSLDIKTLHQVKFRSPWKPDFLGLILRDANFLYVDGHRLADPESFRVLAQK
                     SWDQRGKFSTTFNPCVAVPFGPWDTLARTRTKIIINGEQLKADPETFTVVRWMPGSLL
                     TWRDKSGLHRKVLNQGNLDKDRANSCAAFTLQEHSVSWRKGPECQQEVLPGLDPEQFH
                     PLSNTVAQYQDKLYTIKKTGFGENRLNIVTLDNPGLVIDKRFNAGRTHGYLLTKLPAD
                     GEEEDGLQVFDSSGPLILMDYRVPDEHEAHLSDSPHYKKWYAHDDRYVYAFDGSQLWR
                     YPTPNTKAVRVKWKTKDSGYGYWANYRSGELDGRLLEDGAFIPTGIPYNSSVNIAQYS
                     DGEGSFLVGETDVSWRKLRSTDQQWSQWEKLPDIDPKAFHPITDRVAQYKNHLYIAKL
                     SPFGEDTLEIITLDSSAPFLKQRINAGKGHGYFMRSSRLRDDIQIFAIDGPLKTTERF
                     AYDNRYVYTWIGSQLYRTASPCPARTRNLDQNMFSSNDDIIILTTEEECHKTPDIEQT
                     LKL"
     gene            complement(10211..10765)
                     /locus_tag="ACHKBL_002389"
     CDS             complement(10211..10765)
                     /locus_tag="ACHKBL_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3304 domain-containing protein"
                     /protein_id="HFO5819250.1"
                     /translation="MGLFTQLEKFDQKLTRGYARWGRWVWRTLIVVPVLVVLWNIGQA
                     VWGGPRGGVILEIHSEIDRPILGFSVNGVAGANAFANGGGSITCCGDVSGDTAEVIWT
                     LSTTRTQYNAGMRLEKRNMTLPLPKREWGENYLHVHFMPGDKVLLGWSKDSFSPYEDL
                     HNGGYKTRVRQDVKDKLYGTGKMN"
     gene            11107..11310
                     /gene="yifL"
                     /locus_tag="ACHKBL_002390"
     CDS             11107..11310
                     /gene="yifL"
                     /locus_tag="ACHKBL_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFO5819251.1"
                     /translation="MKNVFKALTVLLTLFSLTGCGLKGPLYFPPADKNAPPPTKPVEM
                     QTQSTVPDKNDRATGDGPSQVNY"
     gene            11347..12171
                     /gene="dapF"
                     /locus_tag="ACHKBL_002391"
     CDS             11347..12171
                     /gene="dapF"
                     /locus_tag="ACHKBL_002391"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HFO5819252.1"
                     /translation="MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQ
                     LLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANG
                     RMVLTVTDDDLVRVNMGEPNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
                     VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACG
                     SGACAAVAVGIQQGLLAEEVRVELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL"
     gene            12168..12875
                     /gene="yigA"
                     /locus_tag="ACHKBL_002392"
     CDS             12168..12875
                     /gene="yigA"
                     /locus_tag="ACHKBL_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 domain-containing protein"
                     /protein_id="HFO5819253.1"
                     /translation="MKQPGEELQETLTELDDRAVVDYLIKNPEFFIRNARAVEAIRVP
                     HPVRGTVSLVEWHMARARNHIHVLEENMALLMEQAIANEGLFYRLLYLQRSLTAASSL
                     DDMLMRFHRWARDLGLAGASLRLFPDCWRLGAPSNHTHLALSRQSFEPLRIQRLGQEQ
                     HYLGPLNGPELLVVLPEAKAVGSVAMSMLGSDADLGVVLFTSRDASHYQQGQGTQLLH
                     EIALMLPELLERWIERV"
     gene            12872..13768
                     /gene="xerC"
                     /locus_tag="ACHKBL_002393"
     CDS             12872..13768
                     /gene="xerC"
                     /locus_tag="ACHKBL_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HFO5819254.1"
                     /translation="MTDLHTDVERYLRYLSVERQLSPITLLNYQRQLEAIINFASENG
                     LQSWQQCDVTMVRNFAVRSRRKGLGAASLALRLSALRSFFDWLVSQNELKANPAKGVS
                     APKAPRHLPKNIDVDDMNRLLDIDINDPLAVRDRAMLEVMYGAGLRLSELVGLDIKHL
                     DLESGEVWVMGKGSKERRLPIGRNAVAWIEHWLDLRDLFGSEDDALFLSKLGKRISAR
                     NVQKRFAEWGIKQGLNNHVHPHKLRHSFATHMLESSGDLRGVQELLGHANLSTTQIYT
                     HLDFQHLASVYDAAHPRAKRGK"
     gene            13768..14484
                     /gene="yigB"
                     /locus_tag="ACHKBL_002394"
     CDS             13768..14484
                     /gene="yigB"
                     /locus_tag="ACHKBL_002394"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase YigB"
                     /protein_id="HFO5819255.1"
                     /translation="MRFYRPLGRISALTFDLDDTLYDNRPVILRTEREALTFVQNYHP
                     ALRSFQNEDLQRLRQAVREAEPEIYHDVTRWRFRSIEQAMLDAGLSAEEASAGAHAAM
                     INFAKWRSRIDVPQQTHDTLKQLAKKWPLVAITNGNAQPELFGLGDYFEFVLRAGPHG
                     RSKPFSDMYFLAAEKLNVPIGEILHVGDDLTTDVGGAIRSGMQACWIRPENGDLMQTW
                     DSRLLPHLEISRLASLTSLI"
     gene            14568..16730
                     /gene="uvrD"
                     /locus_tag="ACHKBL_002395"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             14568..16730
                     /gene="uvrD"
                     /locus_tag="ACHKBL_002395"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HFO5819256.1"
                     /translation="MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIA
                     WLMSVENCSPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHH
                     MDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ
                     SYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNIL
                     VDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAET
                     IRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVV
                     NRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDAL
                     SYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACRELLQEKAL
                     AGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIEN
                     LEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTLHSAKGLE
                     FPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKE
                     VYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFG
                     EGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLETV"
     gene            complement(16775..17677)
                     /locus_tag="ACHKBL_002396"
     CDS             complement(16775..17677)
                     /locus_tag="ACHKBL_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosyl-l-methionine hydroxide
                     adenosyltransferase family protein"
                     /protein_id="HFO5819257.1"
                     /translation="MKKTLATLFLLTCLGSSSAYADNALILQTDFSLKDGAVSAMKGV
                     AFGVDHNLKIFDLTHEIPPYNIWEGAYRLYQTASYWPQGSVFVSVVDPGVGTDRKSVV
                     LKTKNGQYFVSPDNGTLTLVAQTLGIDSVREIDEKANRLKGSEKSYTFHGRDVYAYTG
                     ARLASGAITFEQVGPELPAKVVELSYQKAKATKGEVKGNIPILDIQYGNVWSNISDEL
                     LKQAGIKLNDTLCVTISEGSQQKYAGKMPYVASFGDVPEGQPMVYLNSLLNVSVALNM
                     DNFAQKHQVASGADWNIDVKKCAK"
     gene            complement(17760..18524)
                     /gene="yigE"
                     /locus_tag="ACHKBL_002397"
     CDS             complement(17760..18524)
                     /gene="yigE"
                     /locus_tag="ACHKBL_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HFO5819258.1"
                     /translation="MAHRLFIGKGMITLNLKRIFLTLTLLPLFAVAADDCAPSDPTLT
                     VQAYTVNPQTERVKMYWQKANGEAWGTLHALLADINSQGQVQMAMNGGIYDESYAPLG
                     LYIENGQQKVALNLASGEGNFFIRPGGVFYVAGDKVGIVRLDAFKTSKEIQFAVQSGP
                     LLMENGVINPRIHPNVASRKIRNGVGINKHGNAVFLLSQQATNFYDFACYAKAKLNVG
                     QLLYLDGTISHMYMKGEAIPWQRYPFVTMISVEQKG"
     gene            18686..18742
                     /gene="ysgD"
                     /locus_tag="ACHKBL_002398"
     CDS             18686..18742
                     /gene="ysgD"
                     /locus_tag="ACHKBL_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YsgD"
                     /protein_id="HFO5819259.1"
                     /translation="MDTPSRYWLTILSSRINS"
     gene            18894..19844
                     /gene="corA"
                     /locus_tag="ACHKBL_002399"
     CDS             18894..19844
                     /gene="corA"
                     /locus_tag="ACHKBL_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HFO5819260.1"
                     /translation="MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEPDDDERLRVQS
                     ELGQSLATRPELEDIEASARFFEDDDGLHIHSFFFFEDAEDHAGNSTVAFTIRDGRLF
                     TLRERELPAFRLYRMRARSQSMVDGNAYELLLDLFETKIEQLADEIENIYSDLEQLSR
                     VIMEGHQGDEYDEALSTLAELEDIGWKVRLCLMDTQRALNFLVRKARLPGGQLEQARE
                     ILRDIESLLPHNESLFQKVNFLMQAAMGFINIEQNRIIKIFSVVSVVFLPPTLVASSY
                     GMNFEFMPELKWSFGYPGAIIFMILAGLAPYLYFKRKNWL"
     gene            complement(19880..>20278)
                     /locus_tag="ACHKBL_002400"
     CDS             complement(19880..>20278)
                     /locus_tag="ACHKBL_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000823788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF943 family protein"
                     /protein_id="HFO5819261.1"
                     /translation="SDAGNSVFLVVDHLPWTDSDKINWYLKHQNEIKNQHPLPEGSWH
                     TWYVIDIGNGFTDYKKYIEGPYEDLYCFPTIKSNDNCIVKNYLMVINEYPYRNTHFFI
                     ADNNEYQLTQENKIERVFNPHDFEKDNFQQ"
BASE COUNT         4735 a         5256 c         5269 g         5018 t
ORIGIN      
        1 cgcaggagaa atattcactc aggaagttat tactcaggaa gcaaagagga ttacagaatt
       61 atctcataac aagtgttaag ggatgttatt tcccggttct ctgtggcata ataaacgagt
      121 agatgctcat tccatctctt atgttcgcct tagtgcctca taaactccgg aatgacgcag
      181 agccgtttac ggtgcttatc gtccactgac agatgtcgct tctgcctcat cagacaccat
      241 ggacacaacg ttgagtgaag cacccacttg ttgtcataca gacctgtttt aacgcctgct
      301 ccgtaataag agcaggcgtt tttttatgta tcagaaaggt cccggaggag cttgcctccg
      361 ggtgagaaag agctactgtg gcgggttgtt ctgcaacgtt aacatcaaac cgtcgcgacg
      421 catcgctgcg gcttcttccg gcttgtgcag tctgtccagc gcgtcggcaa gccatgcgta
      481 atcgtaggcg tccggacgtt gtttcagcgc tgcgcggaag gcgagcgatg cttcctgcca
      541 ttcgccgtgc ttcatcagcg actgacccag tgtgctccac aacagcgggc gatcgcccac
      601 gtttttgatc tgttggcgca gcactttttc aagctgttcc gggttattgg ttttcagacg
      661 cgggatcggc agcagcaggc gatcgtcata ctggcgtttc aggccgtcga tgataatttg
      721 ctgggcagta tcatgatcgt cacattcaat aagatgttcc gccattgcca cctgtaacgc
      781 cacctgatga cgcgttttcc ggctttggtt tttccaccag ttacgcaaac cttcactacc
      841 gttatcggca cgcgcctgat ccatcaggcc aatccatgcc tgttgttcca gcattgcacg
      901 atgttcttca tcaccaacat gggctttcgc cattgatggg atgatatcca gcagcgaact
      961 ccatgcacca gtgcggatat acgcctgttc cgccagacgt aatacttccg gatggcgtgg
     1021 cgtaacttcc agcagcttat caacgccgtg acgtgctgca tggttttcat tacgggccag
     1081 ttgcagacgt acacgggtga tttctaccgg aatggtgtca ttgccggcca gctccgctgc
     1141 gcgttccaga tgttggttgg cgcgtgcttc atcaccacgt tgttgcgcgg cttcggctgc
     1201 cagtagatag ttcaccaccg gttgttcagc gtgatcggca tttttcgcca tcagcttttc
     1261 aacttgctga taatcgcctt ccgccagttt cagcagcgcc tgttcggtct gcttacgtgc
     1321 acggcgacgc ttacgtccga caaaccaccc acgggtgtgc gcgcctgtgc ggaagattcg
     1381 ccgcagtagc cactcaatgg caaacagcac taccatcgcc agaatcaaaa taatcgcgag
     1441 gcccgtgacg ctagtttcaa tattgtagtt gtcggtctgg atcagcacgt aaccctgatg
     1501 gccggcaatc atcgggccaa ccacgatccc cgcaatcagc aacacaaaga gcaataacac
     1561 ttttagcatg gttattctcc ttgcggcgcg gctgccggag catcagcttg cggtgcaggt
     1621 gcaggtgcag gtgcaggtgc aggtttagct tccgttgtcc ccgctgccgg ttgtgccagc
     1681 aggttacgca cgcgagtttg catcagtttt tccagcatcg cctggctttg cagggtttcc
     1741 ggaagatcca tcgagatatt ttgctggctt aactggtcca cctcgtcgag gaacgctttg
     1801 gtggtggcat catcagtatc gtagtaagca cgtacccagg tggagacgtt ctccagcgcc
     1861 tggcgataag tctcttcctg gtgacgcggt acagcttgtg ctgcgaccag caggcgagag
     1921 cgaatatttt cgcgcagata gatatcctga tttggcgcta acagcggtac ggcggtgtca
     1981 tcacgacggc gaatcgtaat gaagttgtcc ataaagttct gccagctttt ttgcagattg
     2041 atacgccatt cgctgatgga actggaaagc tcttcaccgt cggaatccat cggcgaacca
     2101 tcgctgtcat tatcagccag acgcagatta tctacctgat ttgaaagctg attaagctta
     2161 aggatgatac cgtcataatc cacctgcgat accgcagaaa ggctggcgat atcatcggta
     2221 attgcccgac gaacggtaat cagactcggg tcattcatat ccgccaggct ggcatctgca
     2281 cttttcagca acgctgcagc ggtcgtgacg tcttgatcgc tccacagctt ccgtcctgcg
     2341 agtttcacca ggaaatcagc ctgagccagc agccaggttt tagcatcgct gccggaaata
     2401 gtcgcgacct tttgttggac ttcatccagc tgttttgcca gcgtttcttg ctgacgattc
     2461 gcctgctcaa gctgtacagc ttgttgcttg ataatgcctt ccagctcagc tttttggctc
     2521 tcctgggctt tttgcagtgc cgtaagttgg ttagccaggg catcgctggt ggcagtctga
     2581 ttgacggcct gttgtttacc ccagccatac aaaccgacgc ccgccgccag agcaatagcg
     2641 atagccaccg cgctgagaat caatgcggta ttgttcttac tctttttttc tgttgcgaca
     2701 ggttgtgacg tggtgtccac ggcctccctg gtctcttcaa ccacggcgga ggttttttct
     2761 tgttccgtca ttatggcttc ctgttatgag agttattgta atgcccgtaa aagcgcatcg
     2821 ttgtcagcgt tatcggcgac cttaatgtct tgccagccca gttcccgggc gagtttcgcc
     2881 aaacgctcac tgacgaccaa tagtcgacag cgtagtaacc agtgctcacg ataccattgt
     2941 gggatcagcg accagagttg ctgcaacatt tcaccgctgg taacaacgac cgtcgtcacc
     3001 tcgcgggatt gccagcgcat cgcttcttct gcaccatcgt aatggattac gcatcgttga
     3061 taacattcac aaaaagtgac ctcagcaccg cgcgccgtca gggtatcccc aattagctca
     3121 cgaccgccat tgccacgtaa tatcagcgca cgtttgcccg caatattttg taattcaggt
     3181 aattgtagca agacttcgct gatttcccga tcctgcgggt agagaatctt ctgtccgctt
     3241 acggtatgta gtgccagtgc ggtggtgcgt ccaatggcga aataagtagg tagtcggggc
     3301 catttacgat cttgctgatg cagctgtgat tgggcaaaag caaccgcgtg ttgcgagagg
     3361 gcaaacaaca gatcgctctc ccccagcgcc gccagttgat cagcaagttg tggtaattgt
     3421 cgacccggag aaaactcaat cagtggaaaa tgccaggcca cctgccccag tgtgcgcaga
     3481 cggctcacta actcttctcc agcgggagac gggcgggtga caaggatact catgccgggg
     3541 cgtctccatt atagacttca gcgaggatct cgcgcgcgcc gttattcagt agctcttctg
     3601 ccagcgaaat ccccatttgt tcggcatctt gcggcgcacc gcggcgttca ccgcgaataa
     3661 tctgcgaacc gtccggcgcg ccgaccagcg cacgcagcca gatttcacca tcaataagct
     3721 cggcgtagct accaattggc acctgacatc cgccttcgag acgggtattc atggcgcgtt
     3781 ctgcggtaac gcgcagtgca gtttcgtggt gattcagcgc ggcaagcagc tcgcgagtgc
     3841 gtgtatcatc aaggcggcat tcaataccga ccgcaccttg tcctaccgcc ggaagagaaa
     3901 tctcgggtgg taacgcggcg cgaatacgtg actccagacc taaacgtttt agtccggcta
     3961 cggcaagaat gatggcatcg tattcgccgt tatccagttt actcaggcga gtgccgacgt
     4021 tgccgcgcag ggagcggata atcagatccg ggcggcgttc agccagttgg cactggcgac
     4081 gtaaactgga cgtcccgacg atactgcctg ccggtaacgc atccagactt tcatagttat
     4141 tggacacaaa ggcatcgcga ggatcttcac gctcgcaaat ggtgaccagc cccagacctt
     4201 gcgggaattc aaccggcaca tctttcattg aatgtacggc gatatcggcg cgattctcca
     4261 ggagcgcgac ttccagctct ttaacaaata agccttttcc gcctactttc gccagcggcg
     4321 tatcaagaat cacatcgccg cgcgtcacca tcggtaccag ttcaacgacc aggcccggat
     4381 ggctcgccat caacttgtct ttgacatagt gtgcctgcca gagtgcaagt gggctttggc
     4441 gtgtggcaat tcttaaaaca ttgtctaaca tgcttgttac cgtcattatc atccgtggtc
     4501 catcctaaca tccttgccag agcgatgtca gtgttgtggt gaaacgtaga cgcctgcgca
     4561 aaccgtaaaa tgaggtctgg cagtggatcc tgacaggcgt ttcacgccgt cgtaataagg
     4621 aatttacaga gaataaacgg tgctacactt gtatgtagcg catctttctt tacggtcaat
     4681 cagcaaggtg ttaaattgat cacgttttag accatttttt cgtcgtgaaa ctaaaaaaac
     4741 caggcgcgaa aagtggtaac ggttaccttt gacatacgaa atatcccgaa tgccgcgtgt
     4801 taccgttgat gttggcggaa tcacagtcat gacgggtagc aaatcaggcg atacgtcttg
     4861 tacctctata ttgagactct gaaacagaga ctggatgcca taaatcaatt gcgtgtggat
     4921 cgcgcgcttg ctgctatggg gcctgcattc caacaggtct acagtctact gccgacattg
     4981 ttgcactatc accatccgct aatgccgggt taccttgatg gtaacgttcc caaaggcatt
     5041 tgcctttaca cgcctgatga aactcaacgc cactacctga acgagcttga actgtatcgt
     5101 ggaatgtcag tacaggatcc gccgaaaggt gagcttccaa ttactggtgt atacaccatg
     5161 ggcagcacct cgtccgtagg gcaaagttgt tcctctgacc tggatatctg ggtctgtcat
     5221 caatcctggc tcgatagcga agagcgccag ttactacaac gtaaatgcag cctgctggaa
     5281 agctgggccg cctcgctggg cgtggaagtt agcttcttcc tgattgatga aaatcgcttc
     5341 cgtcataatg aaagcggcag cctggggggc gaagattgtg gctccaccca gcatatactg
     5401 ctgcttgacg aattttaccg taccgccgtg cgtctcgctg gtaagcgtat tctgtggaat
     5461 atggtgccgt gcgacgaaga agagcattac gacgactatg tgatgacgct ttacgcgcag
     5521 ggcgtgctga cgccaaatga atggctggat ctcggtggct taagctcgct ttctgctgaa
     5581 gagtactttg gtgccagcct ttggcagctc tacaagagta tcgattcccc atacaaagcg
     5641 gtgctgaaaa cactgctgct ggaagcctat tcctgggaat acccgaaccc acgtctgctg
     5701 gcgaaagata tcaaacagcg tttgcacgac ggcgagattg tgtcgtttgg cctcgatcct
     5761 tactgcatga tgctggagcg tgttactgaa tacctgacgg cgattgaaga tttcactcgt
     5821 ctggatttag tacgccgctg cttctattta aaagtgtgcg aaaaactcag ccgtgaacgc
     5881 gcctgcgtag gctggcgtcg tgaagtgcta acccagttag tgaaagagtg ggaatgggac
     5941 gatgctcgtc tggcaatgct cgataaccgc gctaactgga agattgatca ggtacgtgag
     6001 gcgcacaacg agttgctcga cgcgatgatg cagagctacc gtaatctgat tcgctttgcg
     6061 cgccgcaata accttagcgt ctccgccagc ccacaggata ttggcgtact gacgcgtaaa
     6121 ctgtatgccg cgtttgaagc gttaccgggt aaagtgacgc tggtaaaccc gcagatttcg
     6181 cccgatctct cggaaccgaa tctgaccttt atttatgtgc cgccgggccg ggctaaccgt
     6241 tcaggttggt atctgtataa ccgcgcgcca aatattgagt cgatcatcag ccatcagccg
     6301 ctggaatata accgttacct gaataaactg gtggcgtggg catggtttaa cggcctgctg
     6361 acctcgcgta cccgcttgta tattaaaggc aacggcattg tcgatttgcc taagttgcag
     6421 gagatggtcg ccgatgtgtc acaccatttc ccgctgcgcc tgcctgcacc gacgccaaaa
     6481 gcgctctaca gcccgtgtga aattcgccat ctggcgatta tcgttaacct ggaatatgac
     6541 ccgacagctg cgttctgcaa tcaggtggtg cattttgatt tccgtaagct ggacgtcttc
     6601 agctttggcg agaatcaaaa ttgcctggta ggtagcgtgg acctgctgta ccgcaactcg
     6661 tggaacgaag tgcgtacgct gcacttcaac ggcgagcagt cgatgatcga agccctgaaa
     6721 accattctcg gtaaaatgca tcaggacgcc gcaccgccag atagcgtgga agtcttctgc
     6781 tacagccagc atctgcgcgg attaatccgt actcgcgtgc agcaactggt ttctgagtgc
     6841 attgaactgc gtctttccag cacccgccag gaaaccgggc gcttcaaggc gctgcgcgtt
     6901 tctggtcaaa cctggggctt attcttcgaa cgcctgaatg tatcggtaca gaaactagaa
     6961 aatgccatcg agttttatgg cgctatttcg cataacaaac tgcacggcct gtcagtgcag
     7021 gttgaaacca atcacgtcaa attaccggcg gtggtggacg gctttgccag cgaagggatc
     7081 atccagttct tcttcgaaga aacgcaagac gagaatggct ttaatatcta cattctcgac
     7141 gaaagcaacc gggttgaggt gtatcaccac tgcgaaggca gcaaagagga gctggtgcgt
     7201 gacgtcagtc gcttctactc gtcatcgcac gaccgtttta cctacggctc aagcttcatc
     7261 aacttcaacc tgccgcagtt ctatcagatt gtgaaggttg atggtcgtga acaggtgatt
     7321 ccgttccgca ccaaatcgat tggcaacatg ccgcctgcca atcaggatca cgacacgccg
     7381 ctattacagc agtatttttc gtgatgagcg tgccggaaag cgaggcttat ccggcatgca
     7441 atcttagcgg aaactgactg tttcacccgc ttgctgcgtt gccgcctgtt ccagcaaatc
     7501 ccagaaggtt tcgccgctac gatcgcaaat ccactcatcg cctttcagat caaaatggta
     7561 gccgccctgt ttggttgcca gccatacctg gtgcagcggt tcctggcggt tgataatgat
     7621 tttgctcccg ttttcaaagg taatggtcag tacaccgccg ttgatttcgc agtcgatatc
     7681 gctatcgcca tcccagtcgt ccagacgctc ttcaatggtc agccacagtt gatcagccag
     7741 gcgatgaaat tcactgtcgt tcattgttgt atcctgtttt taagtgatgg cggcagtata
     7801 gcggcatggg gtcagagctt caaagtttgc tcaatgtctg gtgttttgtg gcactcttcc
     7861 tccgtcgtca agatgatgat gtcatcattg gatgaaaaca tgttctgatc cagattacgt
     7921 gtcctggccg gacagggcga tgcggttcga taaagttggc taccgatcca ggtataaaca
     7981 tagcgattgt cataagcgaa acgttcggtg gttttcaatg gtccatcaat agcaaaaatt
     8041 tgaatatcat cgcgtaaacg ggaagagcgc ataaagtaac catggccttt cccggcgtta
     8101 atccgttgtt tcaggaaagg tgcagaagag tcgagcgtta ttatttccag ggtgtcttca
     8161 ccaaaaggag acaatttcgc aatgtacaga tgatttttgt actgcgcgac acgatcggtg
     8221 atgggatgaa atgctttcgg gtcaatatcg gggagtttct cccattgact ccattgctgg
     8281 tctgtagagc gaagcttacg ccagctaaca tcggtttcgc cgacgagaaa agagccttcg
     8341 ccatcagaat attgagcaat gttaacggat gagttatagg ggattcccgt tggaataaac
     8401 gcaccatcct caagaagtct gccgtcaagc tcaccactac ggtagtttgc ccaatagcca
     8461 tatcctgaat ctttggtttt ccatttcacc cgcacggctt tggtattggg agttgggtag
     8521 cgccaaagct gtgagccatc gaatgcgtaa acataacgat cgtcgtgtgc ataccatttc
     8581 ttgtaatggg ggctatcgct aaggtgagcc tcatgctcgt caggaacacg ataatccatc
     8641 aggataagcg gcccggaaga atcgaatacc tggaggccat cttcttcttc accatcagct
     8701 ggtaatttgg tcaataaata gccgtgagtt cttcccgcgt taaagcgttt atctatcacc
     8761 aaaccaggat tatccagtgt gacgatattt agcctatttt cgccaaatcc cgtttttttg
     8821 atggtgtaca gcttgtcctg atactgagca acggtattac taagaggatg aaattgttct
     8881 ggatcgaggc cgggtaggac ttcttgctgg cattctggcc ctttgcgcca tgaaacagag
     8941 tgttcctgca aagtgaacgc cgcgcagcta tttgccctat ctttgtccag atttccctga
     9001 ttcagcacct tccggtgcag gccgctttta tctcgccagg ttaataacga accgggcatc
     9061 cagcgtacga cagtaaatgt ctctgggtcg gctttaagct gttcgccatt gattatgatt
     9121 ttggtccgtg tacgggctaa cgtatcccag gggccaaatg gtacggcaac gcaggggtta
     9181 aacgtggtgg aaaactttcc tcgctgatcc caggacttct gcgccagcac acggaaactc
     9241 tcggggtcag cgaggcgatg tccatcgaca tataagaaat tagcgtcacg taatatcagc
     9301 cccagaaagt caggtttcca gggggaacgg aatttcacct gatgcagcgt tttaatatct
     9361 agactatttt ctccgccatt gtcatcggtg cgcgtgcctt caaaatagag actgtgttta
     9421 tctgcggcaa aatcacccca tgccttgaac gaggcgacat cgacgggagg tttgtctggc
     9481 gggttttgca caacttcacc cgcgtagagg atatagcgac catcgctatg ccaggagtaa
     9541 tgaggatacc aggcctgtgc gccttcatag aggtagtcgg ttgtcagatc agtcagagga
     9601 ggtactttat cccatttgtc tagtgttgtc gggtctatta ataaattggg taaacggcgc
     9661 aaacttttgt aattcacgtc aggaagagaa agatttgttt ctttataagg gcgcaggtat
     9721 accacgcctt ttccctgttt ctggtaaata ttatgttcag gcgcggaaag ccgacatccc
     9781 catgccgacg ataaaaacaa cgaacaagac aacaaacaaa ccacattgcg aaagtgcata
     9841 aagccatcct ggcgcgaggt gttgatcacg aaactgctag cagaacataa atcctcacaa
     9901 gtaagcgtaa ctctcgcagc aaatcgtcac ttttcagaat ccataaaggt catcgctatt
     9961 ttctgatagt tgctggaata aacgtgatat tttcattcac gatataaaca caatcccgga
    10021 actgccccat agtacggcta atagcataaa aatattccgg attgacccct ggaagggagg
    10081 caatgtggga ttccgccccc cgttgccttc tttattgtct ttcacgagtt gtacccaggt
    10141 tccaccacgg gtatctaaca ttcgccacat tattacgctt tctgccgaac gtgattttta
    10201 tagaaatcta tcaattcatt ttccctgtac cataaagttt atccttcaca tcctgccgaa
    10261 cgcgggtctt gtaccccccg ttgtgaagat cttcatacgg agaaaaagaa tctttactcc
    10321 accccagcaa cactttatcg ccaggcataa aatgcacgtg caggtaattt tctccccatt
    10381 ctctttttgg gagaggtaac gtcatattgc gtttctccag acgcattcca gcattatact
    10441 gtgtacgagt agtacttaat gtccatataa cctctgccgt atccccactg acatcaccgc
    10501 aacaggtgat tgaaccgcca ccattcgcaa aagcattcgc gcccgccacg ccgttaacac
    10561 tgaatcccag tatcggacgg tcaatttcac tgtggatttc cagtatcact ccacctcttg
    10621 gtcctcccca tactgcctgt cctatattcc acaacacgac cagtactgga acgacaatta
    10681 acgtccgcca tacccagcgc ccccagcggg cataaccgcg cgtaagcttt tggtcaaatt
    10741 tttccagttg ggtaaatagc cccatcgatt ttattccctc ttctcacctg gttggcaaag
    10801 ttgcacgtca ctggcgacta aggtcagaca acgaaatttc gtggtacgcc actctcttaa
    10861 aagtcccatg caggcgataa gaacaagaca acaaacaaac cacattgcga tagtgcataa
    10921 agccatcctg gcgcgaggtg ccgatcacga aactaccagc aaaacataaa tcgccacgtg
    10981 taagcgttat actcgcagca tttcctcact tttcagactt cataaagagt cgctaaacgc
    11041 ttgcttttac gccttctcct gcgatgatag aaagcagaaa gcgatgaact ttacaggcaa
    11101 tccataatga aaaacgtgtt taaggcactc actgtattac ttactctctt cagcctgacg
    11161 ggctgcggtc tgaaaggtcc gctctatttc ccgcctgcag ataaaaacgc accgccgccg
    11221 accaaaccgg tagagatgca aacgcaatcc acggtgccgg ataaaaacga ccgcgccact
    11281 ggcgatggtc catcccaggt gaattactaa aagtcagttt ctgtacccgc gtgattggag
    11341 taaatgatgc agttctcgaa aatgcatggc cttggcaacg attttatggt cgtcgacgcg
    11401 gtaacgcaga atgtcttttt ttcaccggag ctgattcgtc gcctggcgga tcggcacctg
    11461 ggggtagggt ttgaccagct gctggtggtt gagccgccgt atgatcctga actggatttt
    11521 cactatcgca ttttcaatgc tgatggcagt gaagtggcgc agtgcggcaa cggtgcgcgc
    11581 tgctttgccc gttttgtgcg cctgaaaggg ctgactaata agcgcgatat ccgcgtcagc
    11641 accgccaacg ggcggatggt tctgaccgtc accgatgatg atctggtccg cgtaaatatg
    11701 ggcgaaccca acttcgaacc ttccgccgtg ccgtttcgcg ctaacaaagc ggaaaagacc
    11761 tatattatgc gcgccgccga gcagacaatc ttatgcggcg tggtgtcgat gggaaatccg
    11821 cattgcgtga ttcaggtcga tgatgtcgat accgcggcgg tagaaacgct tggtcctgtt
    11881 ctggaaagcc acgaacgttt cccggagcgc gccaatatcg gttttatgca agtggttaag
    11941 cgcgagcata ttcgtttacg cgtttatgag cgtggggcag gagaaaccca ggcctgcggc
    12001 agcggcgcgt gtgcggcggt tgcagtaggg attcagcaag gtttgctggc cgaagaagta
    12061 cgcgtggaac tccccggcgg tcgtcttgat atcgcctgga aaggtccggg tcacccgtta
    12121 tatatgactg gcccggcggt acatgtctac gacggattta ttcatctatg aagcaaccag
    12181 gggaagaact gcaggaaaca ctcacggagc ttgatgaccg ggcggttgtc gattatctga
    12241 ttaaaaatcc tgagtttttt atccgcaatg cgcgcgcagt agaagcgata cgtgtgccgc
    12301 atccggtacg cggcaccgtt tcgttggtcg agtggcacat ggcccgcgca cgtaatcaca
    12361 ttcatgttct ggaagagaac atggcgttgt tgatggagca ggctatcgcc aacgaaggcc
    12421 tgttttatcg cttactctac ttgcagcgta gtctcaccgc cgccagcagt ctcgacgata
    12481 tgctgatgcg ctttcaccgc tgggcgcgcg atctcggcct ggcgggtgcg agcctgcgcc
    12541 tgttcccgga ttgctggcgt ttaggtgcgc cgtcgaacca cactcatctg gcattaagcc
    12601 gtcagtcttt cgaaccgctg cgtattcagc gtttggggca ggaacagcac tatcttgggc
    12661 cgcttaacgg accggagctg ctggtggtgc taccggaagc gaaagcggtg ggatcggtgg
    12721 cgatgtcgat gctgggaagc gatgctgatt tgggtgtcgt gctgtttacc agtcgcgatg
    12781 ccagtcacta tcaacaaggg cagggaacgc agttacttca tgaaattgcg ctgatgttgc
    12841 cggagcttct ggagcgttgg attgaacgcg tatgaccgat ttacacaccg atgtagaacg
    12901 ctacctacgt tatctgagcg tggagcgtca gcttagcccg ataaccctac ttaactatca
    12961 gcgtcagctt gaggcgatca tcaattttgc cagtgaaaac ggcctgcaaa gctggcagca
    13021 atgtgatgtg acgatggtgc gcaatttcgc tgtacgcagc cgccgtaaag ggctgggagc
    13081 tgcaagtctg gcgttacggc tttctgcgct acgtagcttt tttgactggc tggtcagcca
    13141 gaacgaactc aaagccaacc cggcgaaagg tgtttcggca ccgaaagcgc cgcgtcatct
    13201 gccgaaaaac atcgacgtcg atgatatgaa tcggctgctg gatattgata tcaacgatcc
    13261 cctcgccgtg cgcgatcggg caatgctgga agtgatgtac ggcgcgggtc tgcgtctttc
    13321 cgaactggtg gggctcgata tcaaacacct cgacctggag tccggcgaag tgtgggtgat
    13381 gggaaaaggc agcaaagagc gccgcctgcc gattggtcgc aacgctgtgg cgtggattga
    13441 gcactggctt gatttgcgcg acctgtttgg tagcgaagac gacgcgcttt ttctgtcgaa
    13501 actgggcaag cgtatctccg cgcgtaatgt gcagaaacgc tttgccgaat ggggcatcaa
    13561 acaagggctg aataatcacg ttcacccgca taaattacgt cactcgttcg ctacgcatat
    13621 gctggagtcg agcggcgatc ttcgtggtgt gcaggagctg ctgggtcatg ccaacctctc
    13681 caccacgcaa atctatactc atcttgattt tcaacacctt gcctcggtgt acgatgcggc
    13741 gcatccacgc gccaaacggg ggaaataatg cgtttttacc ggcctttggg gcgcatctcg
    13801 gcgctcacct ttgacctgga tgataccctt tacgataacc gtccggtgat tttgcgcacc
    13861 gagcgagagg cgcttacctt tgtgcaaaat tatcatccgg cgctgcgcag cttccagaat
    13921 gaagatctgc aacgcctgcg ccaggcggta cgggaagcgg aacccgagat ttatcacgac
    13981 gtgacgcgct ggcgttttcg ttcgattgaa caagccatgc tcgacgccgg gctgagtgcc
    14041 gaagaagcca gtgcaggcgc acacgcagca atgatcaact ttgccaaatg gcgcagccga
    14101 atcgatgtcc cgcagcaaac ccacgacacc ttaaaacagc tggcgaagaa atggccgctg
    14161 gtggcgatca ccaacggtaa cgcccagccg gagctgtttg gtttagggga ttattttgag
    14221 tttgtgttgc gcgctggccc gcacgggcgc tcaaaaccgt tcagcgatat gtacttcctg
    14281 gcggcggaaa aactcaacgt gccgatcggc gagatcttac atgttgggga cgatctcacc
    14341 actgacgtgg gtggggcaat tcgcagcgga atgcaggcct gttggatcag accggaaaat
    14401 ggcgatctga tgcaaacctg ggacagccgt ttactgccgc atctggaaat ttcccggttg
    14461 gcatctctga cctcgctgat ataatcagca aatctgtata tatacccagc tttttggcgg
    14521 agggcgttgc gcttctccgc ccaacctatt tttacgcggc ggtgccaatg gacgtttctt
    14581 acctgctcga cagccttaat gacaaacagc gcgaagcggt ggccgcgcca cgcagcaacc
    14641 ttctggtgct ggcgggcgcg ggcagtggta agacgcgcgt actggtgcat cgtatcgcct
    14701 ggctgatgag cgtggaaaac tgctcgccat actcgattat ggcggtgacg tttaccaaca
    14761 aagcggcggc ggagatgcgt catcgtatcg ggcaactgat gggcaccagc cagggcggca
    14821 tgtgggtcgg caccttccac gggctggcgc accgtctgct gcgtgcgcac catatggacg
    14881 ccaatctgcc gcaggatttt cagatcctcg acagcgagga ccagctacgc ctgctcaaac
    14941 gcctaatcaa ggcgatgaac ctcgacgaga agcagtggcc gccgcgccag gcaatgtggt
    15001 acatcaacag ccagaaagat gaaggcctgc gtccacatca tattcaaagc tacggtaatc
    15061 cggtggagca gacctggcag aaggtgtatc aggcgtatca ggaagcgtgt gaccgcgcgg
    15121 gcctggtgga cttcgccgag ctgctgctac gtgctcacga gttgtggctt aacaagccgc
    15181 atatccttca gcactatcgc gaacggttta ccaatatcct ggtggatgaa ttccaggata
    15241 ccaacaacat tcagtatgcg tggatccgcc tgctggcggg cgataccggc aaagtgatga
    15301 tcgttggcga tgacgaccag tcaatctacg gctggcgtgg ggcgcaggtc gagaatattc
    15361 agcgtttcct caatgatttc cccggtgctg aaactatccg tctggagcag aactaccgct
    15421 ctaccagcaa tattctgagc gccgctaacg ccctgattga aaacaataac gggcgtctgg
    15481 gtaaaaaact gtggaccgat ggcgcggacg gtgagcctat ttccctctat tgcgctttta
    15541 acgaactcga cgaagcgcgt tttgtggtta accgtatcaa gacctggcag gacaatggcg
    15601 gggcgcttgc cgagtgcgcc attctctacc gcagcaacgc ccagtcgcgt gtactggaag
    15661 aagcactgtt gcaggcgagt atgccgtacc gtatttacgg cgggatgcgc ttcttcgaac
    15721 gccaggaaat caaagatgcg ctctcgtatc tgcgcctgat tgccaaccgt aatgacgacg
    15781 cggcgtttga gcgcgtggtg aatacaccaa cgcggggtat tggtgaccgg acgctggacg
    15841 tggtacgtca gacatcgcgc gatcgccagt taacactctg gcaggcatgt cgtgaactgt
    15901 tgcaggaaaa agccctcgcc ggacgtgctg ccagtgcctt acagcggttt atggaactga
    15961 tcgacgcctt agcgcaggaa actgccgata tgccgctgca tgtacagact gaccgggtaa
    16021 ttaaagactc cggcctgcgc acaatgtacg agcaggagaa aggcgaaaaa ggccagacgc
    16081 gtatcgaaaa cttagaggaa ctggtaacgg cgacgcgcca gttcagctac aacgaagaag
    16141 acgaagattt aatgccgctg caggcgttcc tctcccatgc ggcactggaa gcgggcgagg
    16201 ggcaggcgga tacctggcag gatgcggtgc agttgatgac gctacactcg gcgaaaggtc
    16261 tggaattccc gcaggtgttt atcgttggta tggaagaggg catgttccca agccagatgt
    16321 cgctggatga aggcgggcgt ctggaagaag aacgccgtct ggcctacgtt ggcgtaaccc
    16381 gcgcgatgca gaaactgacg ctgacctacg cggaaacccg ccgtctatat ggcaaagagg
    16441 tttaccatcg cccgtcgcgc tttatcggtg agttgccgga agagtgtgtg gaagaggtgc
    16501 gcctgcgcgc cacggtaagc cgcccggtca gccatcaacg gatggggacg ccgatggtcg
    16561 agaacgacag cggctacaag ctcggccagc gcgtacgcca cgctaagttt ggtgaaggca
    16621 ccatcgtcaa tatggaaggc agcggtgagc atagccgttt gcaggtggcg ttccagggcc
    16681 agggtattaa atggctggtg gcggcttacg cccggttgga gacggtgtga cgttgcggga
    16741 tgcggtgcgc cacaccgcat tccgcatcag caccttactt agcgcacttc ttcacatcaa
    16801 tattccagtc agcaccagac gcaacctgat gtttctgcgc aaaattatcc atattcagcg
    16861 ccacggaaac attcaataag ctgttcagat acaccatcgg ctggccttcc ggcacatcgc
    16921 cgaagcttgc gacatacggc attttccctg cgtatttttg ctgggaacct tcggaaatgg
    16981 tcacacacaa cgtgtcgttg agtttgattc ctgcctgttt cagcaactcg tcgctgatat
    17041 tgctccagac gttgccgtac tgaatatcga gaatcgggat attgcccttc acttcacctt
    17101 ttgttgcttt tgctttctga taagaaagtt cgacaacttt tgcaggaagc tctggcccca
    17161 cctgttcgaa cgtaatcgct ccggaagcca gacgcgcacc agtgtaggca tatacatcgc
    17221 gaccatggaa ggtatatgac ttttcagaac ctttcaggcg gttagctttt tcgtcgattt
    17281 cccggacgct atcaataccc agagtttgtg ccaccaaagt gagcgtaccg ttatctggtg
    17341 aaacgaagta ttgaccgttt ttggttttca gcactaccga tttacggtca gtgcctacgc
    17401 ccggatcgac cacgctaaca aacaccgaac cttgcggcca gtaactggca gtctgataca
    17461 ggcgataagc gccttcccag atgttatacg gtggaatttc gtgtgttaaa tcaaagattt
    17521 taagattatg gtcaacgccg aatgcgaccc ctttcatcgc cgagacggca ccatctttca
    17581 ggctaaaatc cgtttgtaaa atcaaagcgt tatccgcata agcggatgat gaccccagac
    17641 aggttaataa aaataatgtt gcgagcgttt ttttcatttt tgtttccctt catttatctg
    17701 atgattttcc agctgtgtag ccagccccag tttgtctggc ctcctcgagt gtacaatgtt
    17761 taacctttct gctcgacgga aatcatggtg acaaaaggat agcgttgcca cgggattgcc
    17821 tcgcctttca tatacatatg tgaaattgtg ccatcaagat aaagcagctg cccaacgttc
    17881 agtttcgctt tggcataaca ggcaaaatca taaaaatttg tcgcctgctg gctcaacaaa
    17941 aacacggcgt tcccatgttt attaatccca acaccgttac gaattttacg tgaggcgacg
    18001 ttgggatgaa tacgcggatt aattacaccg ttttccatca acaatggccc tgactgcacc
    18061 gcaaactgaa tctctttact ggttttaaag gcatccagac gaacgatgcc gactttatct
    18121 cccgcgacat aaaacactcc gccaggacga ataaagaaat tcccttcacc tgaagcgaga
    18181 tttaacgcca ccttctgctg accgttttcg atgtacaaac cgagcggcgc atagctttca
    18241 tcatagatgc cgccgttcat cgccatctgc acctgaccct gactattaat atccgccagc
    18301 agagcatgta acgttcccca cgcttcgcca ttggcttttt gccagtacat tttcacccgc
    18361 tctgtttgag gattaacggt atacgcctgt acggtcagcg tcggatctga gggtgcgcaa
    18421 tcatcagcgg caacagcaaa caagggaagt aaggtgaggg tgagaaaaat ccgtttgaga
    18481 ttcaaggtga tcattccttt accaatgaat agccgatgcg ccattatagg ccctggatgt
    18541 gggatttttt tatcctgtta gcgaccttga cgagtaccaa aaagcgcgaa gttcaactat
    18601 tgttctgtgg tgttctgttg cgtgttgacg gcaaaatttt gctggcgtaa catgcgcgca
    18661 cgatcactct aagaggacat tcgccttgga cacacccagt agatactggc tcactatcct
    18721 gtcatccagg atcaactcct aaggctatcc ctttttgctg atagccttag cggttgtcag
    18781 cgacctcaat ttttcccgtc gcgctgagtc aggctgttta atggtctgaa acccaatttg
    18841 tttctgtgtg cccaccgaac tgtccgatat tttaagcatt gggagtcccg gtcatgctga
    18901 gcgcatttca actggaaaat aaccgactga cccggctgga agtcgaagag tcacaacccc
    18961 ttgtaaatgc agtatggatt gatcttgtcg aaccggacga cgacgagcga ctgcgcgtac
    19021 aatctgaact tggtcagagc ctggcaaccc gcccggaact ggaagacatc gaagcatcgg
    19081 cacgtttctt tgaagacgat gacggcctgc atattcactc cttcttcttc tttgaagatg
    19141 cggaagatca cgccggtaac tctactgtgg catttaccat ccgtgatggt cgtctgttta
    19201 ctctgcgtga gcgtgaactg cctgcattcc gtctgtatcg tatgcgcgcc cgtagccagt
    19261 caatggtgga cggtaacgcc tacgagctgc tgttggatct gttcgaaacc aaaatcgaac
    19321 agttggcgga tgaaattgaa aacatctaca gcgatctgga gcagttgagc cgggtgatta
    19381 tggaagggca tcagggcgat gagtacgacg aggcgctctc cacgctggcg gaactggaag
    19441 atatcggctg gaaagttcgc ctgtgtctga tggataccca gcgtgcgctc aacttcctgg
    19501 tacgtaaagc acgcttaccg ggtgggcaac tggagcaggc gcgtgaaatc ctgcgagata
    19561 tcgaatccct gctgccgcat aacgaatccc tgttccagaa ggtgaacttc ctgatgcagg
    19621 cggcgatggg ctttatcaat atcgagcaga accgcatcat caaaatcttc tcggtggtat
    19681 ccgtggtgtt cctgccaccg acgctggtcg cttccagcta cgggatgaac tttgagttta
    19741 tgccggaact gaagtggagc ttcggctacc caggcgcgat tatctttatg atcctcgcgg
    19801 gcctggcacc gtatctgtac tttaagcgga agaactggtt gtaatataaa tgccgggtaa
    19861 agattgttct ttatccggct tattgctgaa aattgtcttt ttcaaagtca tgcgggttga
    19921 acactcgctc tatcttattt tcctgggtta gttgatactc attgttatca gcaataaaaa
    19981 aatgagtatt acggtaagga tattcattaa taaccattaa atagtttttt acgatgcaat
    20041 tatcattgct cttaatcgtc gggaaacagt agagatcttc atacggccct tcgatatatt
    20101 ttttatagtc agtgaagcca ttaccgatat caataacata ccaggtatgc caactacctt
    20161 ctggtagggg atgttgattt ttaatttcat tctgatgctt cagataccaa tttattttat
    20221 cgctgtcagt ccatggcaga tgatcaacga ccagaaagac tgaattgcca gcatcaga
//
LOCUS       DAZFLD010000078        20220 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.078, whole genome shotgun sequence.
ACCESSION   DAZFLD010000078 DAZFLD010000000
VERSION     DAZFLD010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20220
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.078"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            363..929
                     /gene="mepS"
                     /locus_tag="ACHKBL_002401"
     CDS             363..929
                     /gene="mepS"
                     /locus_tag="ACHKBL_002401"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional murein DD-endopeptidase/murein
                     LD-carboxypeptidase"
                     /protein_id="HFO5819262.1"
                     /translation="MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRAVG
                     SETSSLQASQDEFENLVRNVDVKSRIMDQYADWKGVRYRLGGSTKKGIDCSGFVQRTF
                     REQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHAST
                     SSGVIISSMNEPYWKKRYNEARRVLSRS"
     gene            1110..2666
                     /gene="pdeN"
                     /locus_tag="ACHKBL_002402"
     CDS             1110..2666
                     /gene="pdeN"
                     /locus_tag="ACHKBL_002402"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HFO5819263.1"
                     /translation="MFIRAPNSGRKLLLTCIVAGVMIAILVSCLQFLVAWHKHEVKYD
                     TLITDVQKYLDTYFTDLKSTTDRLQPLTLDTCQQANPELTARAAFSMNVRTFVLVKDK
                     KTFCSSATGEMDIPLKELIPALDINKNVDMAILPGTPMVPNKPAIVIWYRNPLLKNSG
                     VFAALNLNLTPSLFYSSRQEDYDGVALIIGNTALSTFSSRLMNVNELTDMPVRETKIA
                     GIPLTVRLYADDWTWNDVWYAFLLGGMSGTFVGLLCYYLMSVRMRPGREIMTAIKREQ
                     FYVVYQPVVDTQALRVTGLEVLLRWRHPVAGEIPPDAFINFAEAQKMIVPLTQHLFEL
                     IARDAAELEKVLPVGVKFGINIAPAHLHSESFKADIQKLLTSLPAHHFQIVLEITERD
                     MLKEREATQLFAWLHSVGVEIAIDDFGTGHSALIYLERFTLDYLKIDRGFINAIGTET
                     ITSPVLDAVLTLAKRLNMLTVAEGVETPEQARWLSERGVNFMQGYWISRPLPLDDFVR
                     WLKKPYTPQW"
     gene            2748..4562
                     /gene="yejA"
                     /locus_tag="ACHKBL_002403"
     CDS             2748..4562
                     /gene="yejA"
                     /locus_tag="ACHKBL_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFO5819264.1"
                     /translation="MIVRILLLFIALFTFGAQAQTIKESYAFAVLGEPRYAFNFNHFD
                     YVNPAAPKGGQITLSALGTFDNFNRYALRGNAGARTEQLYDTLFTTSDDEPGSYYPLI
                     AESARYADDYSWVEVAINPRARFHDGSPITARDVEFTFQKFMTEGVPQFRLVYKGTTV
                     KAIAPLTVRIELAKPGKEDMLSLFSLPVFPEKYWKDHKLSDPLATPPLASGPYRITSW
                     KMGQNIVYSRVKDYWAANLPVNRGRWNFDTIRYDYYLDDNVAFEAFKAGAFDLRLEND
                     AKNWVTRYTGKNFDKKYIIKDEQKNESAQDTRWLAFNIQRPVFSDRRVREAITLAFDF
                     EWMNKALFYNAWSRTNSYFQNTEYAARNYPDAAELVLLAPMKKDLPPEVFTQIYQPPV
                     SKGDGYDRDNLLKADKLLNEAGWVLKGQQRVNATTGQPLSFELLLPSSSNSQWVLPFQ
                     HSLQRLGINMDIRKVDNSQITNRMRSRDYDMMPRVWRAMPWPSSDLQISWSSEYINST
                     YNAPGVQSPVIDSLINQIIAAQGNKEKLLPLGRALDRVLTWNYYMLPMWYMAEDRLAW
                     WDKFSQPAVRPVYSLGIDTWWYDVNKATKLPSARQQGE"
     gene            4563..5657
                     /gene="yejB"
                     /locus_tag="ACHKBL_002404"
     CDS             4563..5657
                     /gene="yejB"
                     /locus_tag="ACHKBL_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HFO5819265.1"
                     /translation="MGAYLIRRLLLVIPTLWAIITINFFIVQIAPGGPVDQAIAAIEF
                     GNTGVLPGAGGEGVRASHAQTGVGNISDSNYRGGRGLDPEVIAEITHRYGFDKPIHER
                     YFKMLWDYIRFDFGDSLFRSASVLTLIKDSLPVSITLGLWSTLIIYLVSIPLGIRKAV
                     YNGSRFDVWSSAFIIIGYAIPAFLFAILLIVFFAGGSYFDLFPLRGLVSANFDSLPWY
                     QKITDYLWHITLPVLATVIGGFAALTMLTKNSFLDEVRKQYVVTARAKGVSEKNILWK
                     HVFRNAMLLVIAGFPATFISMFFTGSLLIEVMFSLNGLGLLGYEATVSRDYPVMFGTL
                     YIFTLIGLLLNIVSDISYTLVDPRIDFEGR"
     gene            5657..6682
                     /gene="yejE"
                     /locus_tag="ACHKBL_002405"
     CDS             5657..6682
                     /gene="yejE"
                     /locus_tag="ACHKBL_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO5819266.1"
                     /translation="MSRLSPVNQARWARFRHNRRGYWSLWIFLVLFGLSLCSELIAND
                     KPLLVRYDGSWYFPLLKNYSESDFGGPLASQADYQDPWLKQRLENNGWVLWAPIRFGA
                     TSINFATDKPFPSPPSRQNWLGTDANGGDVLARILYGTRISVLFGLMLTLCSSVMGVL
                     AGALQGYYGGKVDLWGQRFIEVWSGMPTLFLIILLSSVVQPNFWWLLAITVLFGWMSL
                     VGVVRAEFLRTRNFDYIRAAQALGVSDRSIILRHMLPNAMVATLTFLPFILCSSITTL
                     TSLDFLGFGLPLGSPSLGELLLQGKNNLQAPWLGITAFLSVAILLSLLIFIGEAVRDA
                     FDPNKAV"
     gene            6684..8273
                     /gene="yejF"
                     /locus_tag="ACHKBL_002406"
     CDS             6684..8273
                     /gene="yejF"
                     /locus_tag="ACHKBL_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter ATP-binding protein
                     YejF"
                     /protein_id="HFO5819267.1"
                     /translation="MTQTLLAIENLSVGFRHQQTVRTVVNDVSLQIEAGETLALVGES
                     GSGKSVTALSILRLLPSPPVEYLSGDIRFHGESLLHASDQTLRGVRGNKIAMIFQEPM
                     VSLNPLHTLEKQLYEVLSLHRGMRREAARGEILNCLDRVGIRQAAKRLTDYPHQLSGG
                     ERQRVMIAMALLTRPELLIADEPTTALDVSVQAQILQLLRELQGELNMGMLFITHNLS
                     IVRKLAHRVAVMQNGRCVEQNNAATLFASPTHPYTQKLLNSEPSGDPVPLPEPASTLL
                     DVEQLQVAFPIRKGILKRIVDHNVVVKNISFTLRAGETLGLVGESGSGKSTTGLALLR
                     LINSQGSIVFDGKPLQNLNRRQLLPIRHRIQVVFQDPNSSLNPRLNVLQIIEEGLRVH
                     QPTLSAAQREQQVIAVMHEVGLDPETRHRYPAEFSGGQRQRIAIARALILKPSLIILD
                     EPTSSLDKTVQAQILTLLKSLQQKHQLAYLFISHDLHVVRALCHQVIVLRQGEVVEQG
                     PCARVFAAPQQEYTRQLLALS"
     gene            complement(8277..8621)
                     /gene="yejG"
                     /locus_tag="ACHKBL_002407"
     CDS             complement(8277..8621)
                     /gene="yejG"
                     /locus_tag="ACHKBL_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejG family protein"
                     /protein_id="HFO5819268.1"
                     /translation="MTSLQLSIVHRLPQNYRWSAGFAGSKVEPIPQNGPCGDNSLVAL
                     KLLSPDGDNAWSVMYKLSQALSDIEVPCSVLECEGEPCLFVNRQDEFAATCRLKNFGV
                     AIAEPFSNYNPF"
     gene            complement(8955..10145)
                     /gene="bcr"
                     /locus_tag="ACHKBL_002408"
     CDS             complement(8955..10145)
                     /gene="bcr"
                     /locus_tag="ACHKBL_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Bcr"
                     /protein_id="HFO5819269.1"
                     /translation="MTTRQHSSFAIVFILGLLAMLMPLSIDMYLPALPVISAQFGVPA
                     GSTQMTLSTYILGFALGQLIYGPMADSFGRKPVVLGGTLVFAAAAVACALAQTIDQLI
                     VMRFFHGLAAAAASVVINALMRDIYPKEEFSRMMSFVMLVTTIAPLMAPIVGGWVLVW
                     LSWHYIFWILAVAAILASAMIFFLIKETLPPERRQPFHIRTTIGNFAALFRHKRVLSY
                     MLASGFSFAGMFSFLSAGPFVYIEINHVAPENFGYYFALNILFLFVMTIFNSRFVRRI
                     GALNMFRSGLWIQFIMAAWMVISALLGLGFWSLVVGVAAFVGCVSMVSSNAMAVILDE
                     FPHMAGTASSLAGTFRFGIGAIVGALLSLATFNSAWPMIWSIAFCATSSILFCLYASR
                     PKKR"
     gene            complement(10173..10868)
                     /gene="rsuA"
                     /locus_tag="ACHKBL_002409"
     CDS             complement(10173..10868)
                     /gene="rsuA"
                     /locus_tag="ACHKBL_002409"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HFO5819270.1"
                     /translation="MRLDKFIAQQLGVSRAIAGREIRGNRVTVDGEIVRNAAFKLLPE
                     HDVAYDGNPLAQQHGPRYFMLNKPQGYVCSTDDPDHPTVLYFLDEPVAWKLHAAGRLD
                     IDTTGLVLMTDDGQWSHRITSPRHHCEKTYLVTLESPVADDTAEQFAKGVQLHNEKDL
                     TKPAVLEVITPTQVRLTISEGRYHQVKRMFAAVGNHVVELHRERIGGITLDADLAPGE
                     YRPLTEEEIASVV"
     gene            11018..12778
                     /gene="radD"
                     /locus_tag="ACHKBL_002410"
     CDS             11018..12778
                     /gene="radD"
                     /locus_tag="ACHKBL_002410"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFO5819271.1"
                     /translation="MIFTLRPYQQEAVDATLNHFRRHKTPAVIVLPTGAGKSLVIAEL
                     ARLARGRVLVLAHVKELVAQNHAKYQALGLEADIFAAGLKRKESHGKVVFGSVQSVAR
                     NLDAFQGEFSLLIVDECHRIGDDEESQYQQILTHLTKVNPHLRLLGLTATPFRLGKGW
                     IYQFHYHGMVRGDEKALFRDCIYELPLRYMIKHGYLTPPERLDMPVVQYDFSRLQAQS
                     NGLFSEADLNRELKKQQRITPHIISQIMEFAVTRKGVMIFAATVEHAKEIVGLLPAED
                     AALITGDTPGAERDVLIEDFKAQRFRYLVNVAVLTTGFDAPHVDLIAILRPTESVSLY
                     QQIVGRGLRLAPGKTDCLILDYAGNPHDLYAPEVGTPKGKSDNVPVQVFCPACGFANT
                     FWGKTTADGTLIEHFGRRCQGWFEDDDGHREQCDFRFRFKNCPQCNAENDIAARRCRE
                     CDTVLVDPDDMLKAALRLKDALVLRCSGMSLQHGHDEKGEWLKITYYDEDGADVSERF
                     RLQTPAQRTAFEQLFIRPHTRTPGIPLRWITAADILAQQALLRHPDFVVARMKGQYWQ
                     VREKVFDYEGRFRRAHELRG"
     gene            12903..13187
                     /gene="rplY"
                     /locus_tag="ACHKBL_002411"
     CDS             12903..13187
                     /gene="rplY"
                     /locus_tag="ACHKBL_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HFO5819272.1"
                     /translation="MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAVELDH
                     DKVMNMQVKAEFYSEVLTIVVDGKEIKVKAQDVQRHPYKPKLLHIDFVRA"
     gene            complement(13326..14333)
                     /gene="yejK"
                     /locus_tag="ACHKBL_002412"
     CDS             complement(13326..14333)
                     /gene="yejK"
                     /locus_tag="ACHKBL_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009295 - nucleoid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HFO5819273.1"
                     /translation="MSLDINQIALHQLIKRDEQNLELVLRDSLLEPTETVVEMVAELH
                     RVYSAKNKAYGLFSEESELAQTLRLQRQGEEDFLAFSRAATGRLRDELAKYPFADGGF
                     VLFCHYRYLAVEYLLVAVLSNLSSMRVNENLDINPTHYLDINHADIVARIDLTEWETN
                     PESTRYLTFLKGRVGRKVADFFMDFLGASEGLNAKAQNRGLLQAVDDFTAEAQLDKAE
                     RQNVRQQVYSYCNEQLQAGEEIELESLSKELAGVSEVSFTEFAAEKGYELEESFPADR
                     STLRQLTKFAGSGGGLTINFDAMLLGERIFWDPATDTLTIKGTPPNLRDQLQRRTSGG
                     N"
     gene            14515..14742
                     /gene="yejL"
                     /locus_tag="ACHKBL_002413"
     CDS             14515..14742
                     /gene="yejL"
                     /locus_tag="ACHKBL_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejL family protein"
                     /protein_id="HFO5819274.1"
                     /translation="MPQISRYSDEQVEQLLAELLNVLEKHKAPTDLSLMVLGNMVTNL
                     INTSIAPAQRQAIANSFARALQSSINEDKAH"
     gene            14762..16522
                     /gene="yejM"
                     /locus_tag="ACHKBL_002414"
     CDS             14762..16522
                     /gene="yejM"
                     /locus_tag="ACHKBL_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS biosynthesis-modulating metalloenzyme YejM"
                     /protein_id="HFO5819275.1"
                     /translation="MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWP
                     TTLAGRIYSYVSIIGHFSFLVFATYLLILFPLTFIVGSQRLMRFLSVILATAGMTLLL
                     IDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKL
                     RSLTRRRRFARPLAAFLFIAFIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFL
                     EKHGLLDAQEYQRRLIEQGNPDAVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFE
                     KQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDGILSTRTPAALITAL
                     NQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRTQSDEQTATQWINWLGRYAQEDNR
                     WFSWVSFNGTNIDDSNQQAFARKYSRAAGNVDDQINRVLNALRDSGKLDNTVVIITAG
                     RGIPLSEEEETFDWSHGHLQVPLVIHWPGTPAQRINALTDHTDLMTTLMQRLLHVSTP
                     ASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRGERVKDE
                     KPQLSLLLQVLTDEKRFIAN"
     gene            16597..16673
                     /locus_tag="ACHKBL_002415"
     tRNA            16597..16673
                     /locus_tag="ACHKBL_002415"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:16631..16633,aa:Pro,seq:ggg)
     gene            complement(16799..17558)
                     /locus_tag="ACHKBL_002416"
                     /pseudo
     CDS             complement(16799..17558)
                     /locus_tag="ACHKBL_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            17878..18525
                     /gene="narP"
                     /locus_tag="ACHKBL_002417"
     CDS             17878..18525
                     /gene="narP"
                     /locus_tag="ACHKBL_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite response regulator protein NarP"
                     /protein_id="HFO5819276.1"
                     /translation="MPEATPFQVMIVDDHPLMRRGVRQLLELDPGFEVVAEAGDGASA
                     IDLANRLDIDVILLDLNMKGMSGLDTLNALRRDGVTAQIIILTVSDASSDVFALIDAG
                     ADGYLLKDSDPEVLLEAIRAGAKGSKVFSERVNQYLREREMFGAEEDPFSVLTERELD
                     VLHELAQGLSNKQIASVLNISEQTVKVHIRNLLRKLNVRSRVAATILFLQQRGAQ"
     gene            complement(18560..19612)
                     /gene="ccmH"
                     /locus_tag="ACHKBL_002418"
     CDS             complement(18560..19612)
                     /gene="ccmH"
                     /locus_tag="ACHKBL_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis thiol:disulfide
                     oxidoreductase CcmH"
                     /protein_id="HFO5819277.1"
                     /translation="MRFLLGVLMLMISGSALATIDVLQFKDEAQEQQFRQLTEELRCP
                     KCQNNSIADSNSMIATDLRQKVYELMQEGKSKKEIVDYMVARYGNFVTYDPPLTPLTV
                     LLWVLPVVAIGIGGWVIYARSRRRVRVVPEAFPEQSVPEGKRAGYVVYLPGIVVALIV
                     AGVSYYQTGNYQQVKIWQQATAQAPALLDRALDPKAAPLNEEEMSRLALGMRTQLQKN
                     PGDIEGWIMLGRVGMALGNASIATDAYATAYRLDPKNSDAALGYAEALTRSSDPNDNR
                     LGGELLRQLVRTDHSNIRVLSMYAFNAFEQQRFGEAVAAWEMMLKLLPANDTRRAVIE
                     RSIAQAMQHLSPQESK"
     gene            complement(19609..20166)
                     /gene="dsbE"
                     /locus_tag="ACHKBL_002419"
     CDS             complement(19609..20166)
                     /gene="dsbE"
                     /locus_tag="ACHKBL_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbE"
                     /protein_id="HFO5819278.1"
                     /translation="MKRKVLLIPLIIFLAIAAALLWQLARNAEGDDPTNLESALIGKL
                     VPKFRLESLDNPGQFYQADVLTQGKPVLLNVWATWCPTCRAEHQYLNQLSAQGIRVVG
                     MNYKDDRQKAISWLKELGNPYALSLFDGDGMLGLDLGVYGAPETFLIDGNGIIRYRHA
                     GDLNPRVWEEEIKPLWEKYSKEAAQ"
BASE COUNT         4929 a         5237 c         5009 g         5045 t
ORIGIN      
        1 tgatgatgcg caccgtgctt tcatccatcg cgcgcaaaaa tcattctcta agtaaatgaa
       61 tggattgcat gcgtttcact caatcgtact ttaattgacc aatcccgttt attaactttc
      121 tgtatcactt tttcttataa aaaatcatgt aaaaccgctc gccaagaccg caccaatcgg
      181 gtaatctcga actcgttttg cctcggcggt agattatcct cacagcatat aattttgtgc
      241 gttagtccac agatttggcc ttaaggaatt gtttcaacat gcccaggtaa ttagtctcgt
      301 gtcgcttggc atttttttat aacgatattt gtcgttaagg acttcaaggg aaaacaaaca
      361 acatggtcaa atctcaaccg attttgagat atatcttgcg cgggatcccc gcgattgcag
      421 tagcggttct gctttctgca tgtagtgcaa ataacaccgc aaagaatatg catcctgaga
      481 cacgtgcagt gggtagtgaa acatcatcac tgcaagcttc tcaggatgaa tttgaaaacc
      541 tggttcgtaa tgtcgacgta aaatcgcgaa ttatggatca gtatgctgac tggaaaggtg
      601 tacgctatcg tctgggtggc agcactaaaa aaggcatcga ttgttctggt ttcgtacagc
      661 gtacattccg tgagcaattt ggcttagaac ttccgcgttc gacttacgaa cagcaggaaa
      721 tgggtaaatc tgtttcccgt agtaatttgc gtacgggtga tttagttctg ttccgtgccg
      781 gttcaacagg acgccatgtc ggtatttata tcggcaacaa tcagtttgtc catgcttcca
      841 ccagcagtgg tgttattatt tccagcatga atgaaccgta ctggaagaag cgttacaacg
      901 aagcacgccg ggttctcagc cgcagctaat aaaccgtttg gatgcaatcc cttggctatc
      961 ctgacgagtt aactgaaagc actgcttagg cagtgctttt ttgttttcat tcatcagaga
     1021 aaatgatgtt tccgcgtctt gatccaggct atagtccggt cattgttatc ttttaaatgt
     1081 tgtcgtaatt tcaggaaatt aacggaatca tgttcatacg cgctcccaat tctggacgta
     1141 agctcctgct tacctgcatt gttgcaggcg tgatgattgc gatactggtg agctgccttc
     1201 agtttttagt ggcctggcat aagcacgaag tcaaatacga cacactgatt accgacgtac
     1261 aaaagtatct cgatacctat tttaccgacc tgaaatccac tactgaccgg ctccagccgc
     1321 tgaccttaga tacctgccag caggctaacc ccgaactgac tgcccgcgcg gcgtttagca
     1381 tgaatgtccg tacgtttgtg ctggtgaaag ataaaaaaac attctgttca tctgcgactg
     1441 gtgagatgga cattccacta aaagaattga ttccggcgct cgacattaat aaaaatgtcg
     1501 atatggcgat cttacccggt acgccgatgg tgccgaacaa acccgcaatc gtcatctggt
     1561 atcgcaaccc gttgttgaaa aatagtggcg tgtttgccgc gctgaatctc aatctgacgc
     1621 cttctctctt ttatagttca cggcaggaag attacgatgg cgtcgccctc attattggta
     1681 atactgcgct atctaccttt tcttcacgtt tgatgaacgt taatgaatta accgacatgc
     1741 cggtccgtga aactaaaatt gcgggcattc ctctgaccgt tcggctttat gcagatgact
     1801 ggacatggaa cgatgtgtgg tacgcatttt tactgggtgg catgagtgga actttcgttg
     1861 gacttctctg ctattacctg atgagtgtgc gtatgcgccc aggcagagaa atcatgaccg
     1921 ccatcaagcg cgaacaattt tacgtggtgt atcaaccggt ggtggataca caagctttgc
     1981 gggtaacggg cctggaagta ctgctacgct ggcggcatcc agtagcagga gaaatccccc
     2041 cggatgcctt cattaacttt gccgaagcgc aaaagatgat tgtgccactg actcagcacc
     2101 tgtttgagtt gattgcccgc gatgccgcag aattagaaaa agtactgccg gtaggcgtca
     2161 aatttggcat taacattgcg ccggcccact tgcacagcga aagctttaaa gcggatatcc
     2221 agaaactgct cacttccctg cccgcacacc atttccagat tgtgctggaa attaccgagc
     2281 gcgatatgct gaaagagcga gaagccacac aactcttcgc ctggctgcat tcggtcggcg
     2341 tagaaattgc tattgatgac ttcggcaccg ggcacagcgc gcttatctat cttgagcgtt
     2401 ttacgctcga ttatctgaaa attgatcgtg gatttatcaa cgccatcggt acggaaacga
     2461 tcacttcacc cgtacttgac gcggtgctga cgctggcgaa acgcctcaat atgctgacgg
     2521 ttgctgaagg ggtcgaaacg ccagaacagg cacgatggct aagcgaacgc ggcgttaatt
     2581 tcatgcaagg ctactggatt agtcgcccgt taccgctgga cgattttgtt cgctggctga
     2641 agaaaccgta tacgccgcag tggtaaggtg tgcttacgtc ccttattatt catagtgaaa
     2701 gcatgccgga ttgcggctaa tgatgagtaa aaggaaatcc gttgcagatg attgtgcgca
     2761 tactgctgct gtttatcgct ctgttcacct ttggtgcgca ggcgcagact atcaaggaaa
     2821 gctatgcctt tgccgtactg ggcgaacccc ggtacgcatt taatttcaac cattttgatt
     2881 atgtgaaccc cgccgcgcca aaaggtgggc aaataacgtt gtctgcgctc ggcaccttcg
     2941 ataatttcaa ccgctatgca ctgcgcggca atgcgggcgc acgcaccgag cagctgtacg
     3001 acacgctatt tacgacttcc gatgacgaac caggcagtta ttacccgctg attgctgaaa
     3061 gcgcacgcta tgctgacgat tattcctggg tggaggtcgc tattaatcca cgcgcccgtt
     3121 ttcatgatgg ttcgcccatt actgcccgcg atgtagagtt tacttttcaa aaatttatga
     3181 ccgaaggcgt gccgcaattt cgtctggtct acaaaggcac caccgtcaaa gccattgcgc
     3241 cgttaaccgt gcgcattgag ttagcgaaac ccggcaaaga agatatgctg agcctgtttt
     3301 cgctgccggt ctttccagaa aagtactgga aggatcacaa acttagcgac ccgctcgcca
     3361 cgcctccgct tgccagtggt ccgtaccgaa ttacgtcctg gaaaatgggg caaaatattg
     3421 tctattcccg cgtgaaagat tactgggcag caaacttacc ggtaaaccgt ggacgctgga
     3481 atttcgacac cattcgctac gattattacc tcgatgataa tgtcgccttt gaagcgttta
     3541 aagccggtgc ctttgatttg cgtttggaaa acgacgccaa aaactgggtc acgcgctata
     3601 ccggtaaaaa tttcgataag aaatacatca tcaaagatga gcaaaagaac gaatcagccc
     3661 aggatacgcg ctggctggcg tttaatatcc aacgtccggt attcagcgat cgccgggtgc
     3721 gggaagcaat cactctcgcc tttgactttg aatggatgaa caaggcgttg ttttataatg
     3781 cctggagtcg cacgaacagt tattttcaga ataccgaata tgcggccaga aattaccccg
     3841 acgccgcgga gctggtgctt ctggcaccaa tgaaaaaaga tctaccgcca gaagtcttca
     3901 cgcaaatcta ccagccgccg gtatccaaag gcgatggcta cgatcgtgac aacctgttaa
     3961 aagccgacaa acttctcaac gaagcgggct gggtgctgaa gggtcagcaa cgcgtaaatg
     4021 ccacaacggg tcagccgctc agctttgagt tattgctacc ttcgagcagc aatagtcagt
     4081 gggtattgcc gttccagcac agcctgcaac ggctgggtat caacatggac attcgcaaag
     4141 tggataactc gcaaatcacc aaccgcatgc gcagtcgcga ctatgacatg atgccgcgcg
     4201 tatggcgggc gatgccgtgg cccagttccg atttacagat ttcctggtca tcggaatata
     4261 tcaattccac ttataatgcc cccggcgtgc aaagcccggt tatcgactcg ctgatcaacc
     4321 aaattattgc cgcgcaggga aataaagaaa aattactgcc gttggggcga gcactggatc
     4381 gcgtattaac gtggaattat tacatgctgc caatgtggta catggcggaa gaccgtctcg
     4441 cctggtggga taaattctcc caacccgctg tacgccctgt ttacagcctg ggtattgata
     4501 cctggtggta tgacgttaat aaagcgacga aactgccgtc agccagacaa cagggagagt
     4561 agatgggcgc ttatctgatt cgccgtctgt tgctggtgat cccaacgtta tgggcgatta
     4621 tcactatcaa ctttttcatc gtgcaaattg cgcctggcgg tccggtcgac caggccatcg
     4681 ccgccattga gtttggtaat actggtgtat tacccggtgc aggcggtgaa ggtgttcgcg
     4741 ccagccatgc acaaacgggc gtcggtaata tcagcgacag taattaccgt ggcggacgcg
     4801 gattggatcc agaagtgatc gccgagatca ctcatcgcta cggtttcgat aagccgatcc
     4861 acgaacgtta cttcaaaatg ctctgggact acatccgttt tgattttggc gacagccttt
     4921 ttcgcagcgc ctcggtgctg acgctgatta aagacagtct gccggtctcc atcaccctcg
     4981 gattgtggag cacgctgatt atctatctgg tgtcgattcc gttaggcatt cgcaaagctg
     5041 tttataatgg gagccgtttt gacgtctgga gtagtgcatt tatcatcatc ggctacgcca
     5101 ttccggcctt tttgtttgcc atcctgctga ttgtcttctt cgcgggcggc agctatttcg
     5161 acctgttccc tctgcgcggc ctggtttccg ctaactttga ttcgctgccg tggtatcaga
     5221 aaatcaccga ttatctgtgg catatcacgc tgccggtgct ggcgacagtg attggcggct
     5281 ttgcggctct gaccatgctg acaaaaaact cattccttga tgaagtgcgt aagcaatacg
     5341 tggtgacggc acgtgcgaaa ggggtaagtg aaaaaaatat tctctggaaa catgtgttcc
     5401 gcaacgccat gctgctggtg attgccggtt ttccggcgac gtttatcagc atgtttttta
     5461 ccggctcgct gctgattgag gtgatgtttt cactcaatgg tcttggctta ctgggctacg
     5521 aagcgaccgt ctcgcgcgat tatcctgtaa tgtttggtac cttgtatatt ttcaccctga
     5581 ttggcctgct gctgaatatt gtcagtgata tcagctatac gctggtcgat ccgcgtatag
     5641 attttgaggg acgttaatgt cgcgactcag ccccgtcaat caggcccgtt gggcgcgttt
     5701 tcgtcataac cgtcgcggct actggtcgtt atggattttc ctcgtcttgt ttggtttgag
     5761 tttgtgttct gaacttatcg ccaacgataa accgttgctg gtgcgttatg acggcagttg
     5821 gtatttcccg ttgttgaaaa actacagcga aagcgatttt ggcggcccgc tggcaagtca
     5881 ggctgattat caggacccgt ggctgaaaca acggctggaa aataacggct gggtactgtg
     5941 ggcaccgatt cgctttggtg ctaccagtat caactttgct accgataagc ccttcccttc
     6001 tccaccctcc cggcaaaact ggctgggaac ggatgccaat ggcggcgatg tgctggcgcg
     6061 cattctctat ggcacgcgga tctcggttct gtttggcctg atgctgactc tctgttccag
     6121 cgtgatgggc gtgctggcgg gggcgctaca aggctattac ggcggtaaag tcgatctctg
     6181 ggggcaacgc tttattgaag tatggtcagg gatgccgacg ctgtttttga ttattttact
     6241 ttccagcgtc gtacagccta acttctggtg gctgctggca attaccgtct tgtttggctg
     6301 gatgagtctg gtcggcgtgg tgcgggcgga gtttttacgt acccgtaatt tcgactacat
     6361 ccgcgcggcg caggcgcttg gcgtcagcga tcgcagtatc atcctgcgtc atatgttgcc
     6421 aaatgccatg gtcgctaccc tcaccttttt accgtttatt ttatgtagtt cgataaccac
     6481 cctgacctca ctcgatttcc tcggcttcgg tctgccgctc ggttcaccgt cactcggcga
     6541 actgctgtta caagggaaaa ataaccttca ggccccgtgg cttgggatca ccgccttctt
     6601 gtcggtggcg atattgttgt ctttgctgat ctttattggt gaagccgtcc gcgacgcatt
     6661 tgatcctaat aaggcggtgt agcatgacgc aaactctgtt agcgattgaa aatttgtcgg
     6721 tgggttttcg ccatcagcaa accgtacgca cagtagtcaa tgatgtttca cttcagattg
     6781 aggctggcga aacgctggcg ctggtgggtg agtcaggttc aggcaaaagc gttaccgcgc
     6841 tgtcaatttt acgcctgctc ccttccccgc cggttgaata tctctccggc gatattcgtt
     6901 ttcatggcga atcgctgctt catgccagcg atcaaacgtt gcgcggtgta cgcggtaata
     6961 agatcgccat gatttttcag gagccgatgg tgtcgttaaa tccattacat accctggaaa
     7021 aacagcttta tgaagtactt tcactccacc gcgggatgcg tcgggaagcg gctcgtggcg
     7081 aaattcttaa ctgccttgat cgcgtcggta tccgccaggc ggcaaaacgg ctgacagatt
     7141 atccgcatca gctctccggc ggcgaacgcc agcgggtgat gattgcgatg gcgctgttaa
     7201 cgcgaccgga attattaatt gccgatgaac caaccaccgc gctggacgtc tctgtccagg
     7261 cgcagatttt acagctgttg cgcgaactgc aaggcgagct gaatatgggt atgctgttta
     7321 ttactcataa cctcagcatt gttagaaaac tggcccaccg cgtggcggta atgcaaaacg
     7381 gtcgctgtgt cgagcaaaat aacgccgcta cgctatttgc ctcacccact catccttaca
     7441 cacaaaagct actcaacagt gaaccgtcag gcgacccggt cccattgcca gaacctgcct
     7501 caacgttgct ggatgttgaa cagcttcagg ttgccttccc cattcgcaaa gggattttga
     7561 agcgcattgt ggatcataat gtggtggtga aaaacatcag ttttacgcta cgagcgggtg
     7621 aaacactggg tttagtgggc gagtctggtt ccgggaaaag cacgacggga ctggcgctgc
     7681 tgcgactgat taactctcag ggcagcatcg tctttgacgg taagccactg caaaatttaa
     7741 atcgccgcca gctgttacct attcgtcatc gcattcaggt ggtatttcag gatccaaact
     7801 cctcgctcaa cccacgactc aacgttttgc agattattga ggaaggctta cgggttcacc
     7861 agccgacgct ttctgccgcg caacgcgaac aacaagtgat agccgtgatg catgaagtgg
     7921 gattagatcc tgaaacacgc caccgttatc cggcggagtt ctctggtggt cagcggcaac
     7981 gtattgcgat tgccagggcg ttaattctta agccctcgct gatcattctt gatgaaccaa
     8041 catcatcact cgacaaaacg gtgcaggcgc aaatattgac gctattgaaa tcattgcaac
     8101 aaaagcatca actggcctat ttgtttatca gccacgattt gcacgttgtc cgcgcgttat
     8161 gtcatcaggt tatcgtactg cgacaagggg aagtagtgga acaaggaccg tgcgcgcgcg
     8221 tgtttgccgc accgcagcag gagtatacgc gtcagctact ggcgttgagc tgacgcttaa
     8281 aaaggattgt agtttgaaaa gggttcggca attgccacac caaaattttt caatcggcat
     8341 gttgcagcaa attcgtcctg gcgatttaca aacaggcacg gctcaccttc acactccagc
     8401 accgaacatg gaacttcgat gtcgcttaac gcctggctta gtttgtacat caccgaccat
     8461 gcattatcac catccgggct aagcaattta agcgccacca ggctgttgtc accgcacggt
     8521 ccattttgcg gaatcggttc aaccttcgaa cctgcgaaac cagcagacca gcgatagttc
     8581 tgcggcagtc gatggacgat tgagagttgt aatgaagtca cttattttcc ccggaagtac
     8641 attttacttc acaatttgtt tacatcaatt ttaacacatc atcaacaatc cgtctttata
     8701 cagatcgtaa agattgatgc ctcgcctgcg tggcctctat ggctctgatt taaataatat
     8761 ctgcgggcgt ggtcacgttt gcggggctat atgtacccgt ttctgcgatc taactcaacc
     8821 tttttaacta caatgatgtg actttttaca taaattgatt ttacataaaa taaacatata
     8881 tcgggggaat gatagatttg tggttgacat gcatcaacaa aggaagcctt ttagcttcct
     8941 cgttgtgcaa tagatcaccg ttttttcggc cgactggcgt acaaacagaa gagaatggag
     9001 ctggttgcgc agaatgcaat tgaccaaatc atcggccatg cagagttaaa ggtcgcaaga
     9061 gaaagtaatg cgccaacaat tgcccctatg ccgaaacgga aggttcctgc cagcgaagat
     9121 gccgttcccg ccatatgggg aaactcatca agaatgaccg ccatcgcatt ggatgacacc
     9181 atcgacacgc agcccacaaa cgccgcaacg ccaaccacca gcgaccagaa tcccagcccc
     9241 agcagtgcac tgatgaccat ccacgctgcc ataataaact gtatccacaa ccccgagcgg
     9301 aacatattta acgcgccaat acggcggacg aagcggctgt taaagatggt catcacgaac
     9361 agaaaaagaa tgtttagcgc aaagtaataa ccaaagtttt ccggcgcgac gtggttgatt
     9421 tcaatgtaga caaacggtcc ggcgcttaag aatgagaaca tcccggcaaa gctgaaaccg
     9481 ctggccagca tataactcag gacacgctta tgacggaaca gcgccgcaaa gttaccaata
     9541 gtggtacgaa tgtgaaaagg ctggcgacgc tccggtggta aggtttcttt gatcagaaag
     9601 aagatcattg ccgaagccag aatcgccgcc acggcgagga tccagaagat gtaatgccag
     9661 ctcagccaca ccagcaccca gccgccaact atcggtgcca tcagcggtgc aatggtcgtc
     9721 accagcatga caaacgacat catccgtgag aactcttctt tcgggtagat atcacgcatc
     9781 aaagcgttga taaccacgct ggccgcagcc gcagccagcc cgtggaagaa acgcatcaca
     9841 atcagctgat cgatggtttg tgccaacgca cacgccaccg cggcggcggc aaacaccagc
     9901 gtaccgccaa gaaccaccgg cttacgcccg aagctgtctg ccatcggccc gtagattaac
     9961 tgccccaacg caaagcccag aatataagta ctgagggtca tctgcgtact gcccgccggt
    10021 acaccaaact gagctgaaat taccggtagc gcaggcagat acatatcaat cgacagcggc
    10081 atcaacatgg ccagcaggcc aaggataaaa acaatagcaa acgacgaatg ctgtcgggtg
    10141 gtcacaacgg gctcctgaaa gtcattgaaa agttagacga cgctggcaat ttcttcttca
    10201 gttaacggac gatattcacc gggagctaaa tcagcatcca gcgtaatgcc gccaatacgt
    10261 tcacgatgca gctcaaccac atggttaccc acggcggcga acatgcgttt cacctgatga
    10321 taacgccctt cgctgatggt cagacgaacc tgcgttgggg taatcacttc cagcaccgcc
    10381 ggcttagtga gatctttttc gttatgcagc tgcacgcctt tagcaaattg ctctgccgta
    10441 tcgtcagcta cgggtgattc cagcgtcacc agataggtct tctcgcaatg atggcgcggg
    10501 gaagtaatgc ggtgcgacca ctgaccatca tctgtcatta gtaccagacc ggtggtatcg
    10561 atatccagac gccctgcagc gtgcagtttc cacgctaccg gttcatcaag aaaatagagc
    10621 accgttgggt gatccgggtc gtccgtggag caaacatagc cctgaggctt attgagcatg
    10681 aagtaacgtg gaccgtgttg ctgcgccagc gggttgccat cgtaagcgac atcatgttca
    10741 gggagcagtt tgaacgctgc attacggacg atttcgccat cgacggtgac acgattgccg
    10801 cggatttcac gcccggcaat agcacggcta acgccgagtt gctgtgcgat aaatttatca
    10861 agtcgcatgt gtgtgatttt gcctgtaaaa aacggaggtc gggtaatgac ccgaaaatct
    10921 gaactgttgt ctgcccagta tagcggtcta acaacatccc tcaagggaaa aagatccatg
    10981 gcatactatt agcagaataa tctacctacg cgagaccatg atttttacac ttcgcccata
    11041 tcagcaagaa gccgtggatg ccacgctcaa ccattttcgt cgtcataaaa cccctgccgt
    11101 tatcgtgctg cccaccggtg caggtaaaag cctggtgata gcggaactgg cgcggctggc
    11161 gcgtggtcgc gtgctggtgc tggcacacgt taaagaactg gtggcgcaaa accatgcaaa
    11221 atatcaggcg ctggggctgg aagccgatat ttttgccgcc gggctaaagc gcaaagagag
    11281 ccacggtaaa gtggtatttg gcagcgtgca gtcggtcgcc cgtaatcttg atgcctttca
    11341 gggtgaattt tcgctgttga ttgtcgatga atgtcaccgt attggtgacg atgaagagag
    11401 ccagtatcag caaatcctca ctcacctgac caaagtgaat ccccacttac gcctgctggg
    11461 gctgactgcc acgccttttc gactgggcaa aggctggatt tatcagttcc attatcacgg
    11521 catggtacgc ggcgatgaga aagccctttt ccgtgactgc atttatgagc taccgctgcg
    11581 ttatatgatt aaacacggct atctgacgcc gccagaacga ctggatatgc cagtagtgca
    11641 atacgatttc agccgcttgc aggcacagag taacgggcta ttcagcgaag ccgatctcaa
    11701 ccgcgagctg aaaaaacagc aacgcattac cccgcacatc atcagccaga ttatggagtt
    11761 tgctgtaacg cgcaaagggg tgatgatttt cgccgccacc gttgaacacg caaaagagat
    11821 tgtgggactg ctgcctgccg aagatgctgc actgattact ggcgacaccc ccggcgctga
    11881 gcgcgatgtg ttaattgaag attttaaagc ccagcgtttt cgctatctgg tcaacgtcgc
    11941 ggtactgacc accggatttg acgccccgca cgtcgatctt atcgccattc tgcgccctac
    12001 cgaatcagtg agtctttacc aacaaattgt cgggcgcggt ctgcgtctcg ctcccggcaa
    12061 gactgattgc ttaattcttg attatgcggg taaccctcac gatctctacg cgccggaagt
    12121 tggtacaccg aaaggcaaaa gtgacaacgt tccggtacag gttttctgcc ctgcctgcgg
    12181 ttttgccaac accttttggg ggaaaacgac cgccgacggg acattgattg aacactttgg
    12241 tcgtcgctgt cagggatggt ttgaagatga cgacggacat cgcgaacagt gtgacttccg
    12301 tttccgtttt aaaaattgcc cgcaatgtaa cgctgaaaat gatattgccg cccgccgctg
    12361 ccgcgaatgt gacacggtac tggttgatcc ggatgatatg ttaaaagcgg cgctacgact
    12421 gaaagacgcg ctggtattac gctgtagcgg catgtctttg cagcatgggc acgacgagaa
    12481 aggcgaatgg ttgaaaatca cctattacga tgaagacggc gcggatgtga gtgagcgttt
    12541 ccgtctgcaa acgcccgccc agcgtaccgc cttcgaacaa ctttttatcc gtccgcatac
    12601 gcgcacaccg ggcatccctc tgcgctggat caccgccgcc gatatcctcg cccagcaagc
    12661 attactgcga cacccggatt ttgtcgtcgc ccgcatgaaa ggtcagtact ggcaagtgcg
    12721 tgaaaaagtg ttcgattacg aaggtcgttt tcgtcgggcg cacgaattac gcggttaatg
    12781 gtagttttga ttgatgtata aggcgattga gtatagaatc tcgcccgctt ttgcatacgc
    12841 aaagcagatc acttacctgt tgctgggtcg cctgtagcag gattaattta agagagaaag
    12901 aaatgtttac tatcaacgca gaagtacgta aagagcaggg taagggtgcg agccgccgcc
    12961 tgcgtgccgc taacaagttc ccggcaatca tctacggtgg caaagaagcg ccgctggctg
    13021 tcgaactgga tcacgacaaa gtcatgaaca tgcaagttaa agctgaattc tacagcgaag
    13081 ttctgaccat cgttgttgac ggtaaagaaa tcaaagttaa agctcaggac gtacagcgtc
    13141 acccgtacaa accgaagctg ctgcacatcg acttcgttcg cgcttaattg ctgaataagt
    13201 tgtaaaaaac cccgctccgg cggggttttt tgtctctgca aattatgcct gattacggta
    13261 ttgctatttt ttgcaggcca gataaggcgt tcacgccgca tctggcattc agaacacaaa
    13321 accgattaat tgccgccaga cgtccggcgc tgcaattgat cgcgcaagtt cggcggcgtg
    13381 cctttaatgg tcagggtatc ggttgctgga tcccagaaga tgcgttcacc taacagcatc
    13441 gcatcaaagt taatagtcaa gccaccgccg ctaccggcaa acttcgtcaa ctgacgcagt
    13501 gtgctacggt ctgccgggaa gctctcttcc agttcgtagc ctttttcagc cgcaaactcc
    13561 gtgaagctca cttcgctgac gcctgccagc tctttcgaca gcgattccag ttcaatctct
    13621 tcacctgcct gcagctgctc attacagtag ctataaacct gctggcgcac gttctgtcgc
    13681 tctgctttat ccagttgtgc ttcggcagtg aaatcatcga ccgcttgcaa cagcccacgg
    13741 ttttgcgctt tcgcgtttaa cccttcgctg gcaccgagga aatccataaa gaaatccgcc
    13801 actttgcgcc ctactcgccc tttcaggaaa gtgagatagc gggtcgactc tggattggtt
    13861 tcccactcgg ttaaatcgat acgcgcaaca atatccgcat gattgatatc aagataatgg
    13921 gttgggttga tgtcgagatt ttcgttaaca cgcatactgc tcaggttgct cagaactgcc
    13981 accagcagat actccaccgc cagatagcga tagtggcaaa acagcacgaa tccaccgtca
    14041 gcaaaaggat atttcgctaa ttcgtcacgc aggcgaccgg ttgccgcgcg gctaaatgcc
    14101 agaaagtcct cttcgccctg acgctgtaag cgcagcgtct gcgccagttc actctcttcg
    14161 ctaaacaagc cgtaagcttt atttttggcg ctatagaccc gatgcagttc tgccaccatc
    14221 tcaacgacgg tttccgtcgg ttccagcaat gaatcgcgca acaccagctc aaggttctgc
    14281 tcatcacgct tgataagctg gtgcagagca atctggttga tatccagact catgataaac
    14341 tctcctttaa gaccgggcgg tattcaacca ccgccggtta agcgacgcaa taaaagatgg
    14401 cggtcatttt acagaaggcg gcgtaacgag aatgatttaa cgcataaaaa agcagaaaaa
    14461 aaaccgttgc tacggtaata tgttgccctt tcatcaacta actgatttcg atttatgcca
    14521 caaatttccc gctacagtga tgaacaggtt gaacaattgc tcgcagagct gctcaacgta
    14581 ctggaaaaac ataaggctcc gaccgacctt tccctgatgg tgttaggcaa catggtcact
    14641 aaccttatca acaccagcat tgccccggcc caacgccagg caattgccaa ctcttttgcc
    14701 cgcgccttac agtcctctat caacgaagac aaagcgcact aagggaaaca gataacaggt
    14761 tatggtaact catcgtcagc gctaccgtga aaaagtctcc cagatggtca gttgggggca
    14821 ctggtttgca ctgttcaata ttctgctttc gctcgtcatt ggcagccgtt acctgtttat
    14881 cgccgactgg ccgacaacgc ttgctggtcg catttattcc tacgtaagca ttatcggcca
    14941 tttcagcttc ctggtgttcg ccacctactt gctgatcctc ttcccgctga cctttatcgt
    15001 cggctcccag aggctgatga ggtttttgtc cgtcattctg gcaacggcgg gaatgacgct
    15061 attactgatc gatagcgaag tctttactcg tttccatctc catcttaatc ccatcgtctg
    15121 gcaactggtt atcaacccag acgaaaatga gatggcgcgc gactggcagc tgatgttcat
    15181 cagcgtgccg gttattttat tgcttgaact ggtgtttgcg acgtggagct ggcaaaagct
    15241 acgcagcctg acgcgtcgtc ggcgcttcgc gcgcccgctg gccgcattct tatttatcgc
    15301 ctttatcgcc tcgcatgtgg tttatatctg ggccgatgcc aacttctatc gcccgatcac
    15361 catgcaacgc gctaacctgc cgctttcgta cccgatgacg gcgcgacgtt ttcttgagaa
    15421 gcatggtctg cttgatgcgc aggagtatca acgccgtctc attgagcaag gtaatccaga
    15481 cgccgtttcc gttcagtatc cgttaagcga actgcgctat cgcgatatgg gcaccgggca
    15541 gaatgtgctg ttgattactg tcgatggcct gaactactca cgcttcgaga agcagatgcc
    15601 tgcgctggca ggttttgctg agcaaaatat ttcgttcacg cgccatatga gctccggcaa
    15661 cactacagac aacggcatct ttggcctgtt ctatggcatc tcgccgagct atatggatgg
    15721 cattctgtcg acccgtacgc ctgcggcatt aattactgcg cttaatcagc aaggctatca
    15781 gctggggtta ttctcgtcag atggctttac cagcccgctg tatcggcagg cattgttgtc
    15841 agatttctcg atgccgagtg tacgcaccca atccgacgaa cagaccgcca cgcagtggat
    15901 caactggctg ggccgctacg cacaagaaga taaccgctgg ttctcgtggg tctctttcaa
    15961 tggcacaaac attgacgaca gcaatcagca ggcatttgca cggaaatata gccgggcggc
    16021 aggcaatgtc gatgaccaga tcaaccgcgt gctcaatgca ctgcgtgatt ctggcaaact
    16081 ggacaatacg gtggtgatta tcactgccgg gcggggtatt ccgctgagcg aagaggaaga
    16141 aacctttgac tggtcccacg gtcatctgca ggtgccatta gtgattcact ggccaggcac
    16201 gccggcgcag cgtattaatg cgctgactga tcataccgat ctgatgacga cgctgatgca
    16261 acgcctgcta catgtcagca cacctgccag cgaatattcg caaggtcagg atttgttcaa
    16321 ccctcaacgc cgccattact gggttaccgc cgcggataac gatacgctgg caattaccac
    16381 cccgaaaaag acactggtgc tgaacaataa cggtaaatac cgcacttaca acttacgtgg
    16441 tgaaagagtg aaagatgaaa aaccacagtt aagtttgtta ttgcaagtac tgacagacga
    16501 gaagcgtttt atcgctaact gattaattat aaatcagtta gcaaaatatc ttacttgcaa
    16561 tcggtgtgga aaacggtagt attagcagcc acgagtcggc acgtagcgca gcctggtagc
    16621 gcaccgtcat ggggtgtcgg gggtcggagg ttcaaatcct ctcgtgccga ccaaaaatcc
    16681 caagaaaacc aacctattgc ggttggtttt tttatatctg catttaattc gataaacaga
    16741 cagcgacaca tcacgacctg tttattttct gttatcagaa cgtccagact acacccgcct
    16801 gagttgtgct gctttcttca cgggagaaac gactatcgat accgagcgtc aggcccgtca
    16861 atgtttgctc aaagccagct cccgcatcag tgttcacgtt gttgcggctg ttaattgccg
    16921 aactccacat cgccgccctt tacgctacca agacgctcac gcacggtgtc cagttctgca
    16981 tcaaatacca gcggttgtgc tgccgccata ttcagcacat ccgtggttga agggggcatt
    17041 tgtacgcgat tctctgtgag actccagcta tggttgccat tatccagcaa ggtatattca
    17101 tacgtactga tatcaacaac gcttccggca ttgcccaacg taaatgccgc atcaccgcgc
    17161 cctcctttaa tcgtgggatc agtcgccgcg ccataaacct gtagcactcc attattgatc
    17221 gtagtctggt ttaccgcgcc acccatttga acaatttgat aaccatcatt taatactgag
    17281 tattctgcgg tgccatttat ttcaatgtac tgatatcccc cgttaatttt agtattacta
    17341 cttacaccaa attcttatat atgctgttct ccgccaggat tgattttcgt attattggcg
    17401 gccccataaa ccagctggat atctttttcc aggacaacac catcaactgt ctcaccatac
    17461 tcaacagttg atgccaatgc tatgggtgaa tataacgctg tagccaccag aacagatatt
    17521 gcggaacgac aaagagaaac agaaccagat tggtgcatta agctttcatc ctatgaaatt
    17581 aattgctgtt aaaagcattg gggacagaaa atacccatag ctccataccc ggagtcagtt
    17641 ttaaaaaact gtttaaagaa atgcacaagt attgtgattg attttttagt tgtttttcct
    17701 gatgagaagc tgatgcaaaa ttccgtcttt ataatgaaga tgatgccaaa gcgaacgaca
    17761 aagttgtagt tttcactaca tgtccataca taaaatgggg taacattcac gcgcctggta
    17821 gcgttaccaa cgctacgctc aaacataatg attctaataa aacctcagga gactactatg
    17881 cctgaagcaa cgccttttca ggtgatgatt gtggatgatc atccacttat gcgacgcggt
    17941 gttcgtcagt tactggagct tgatcctggc tttgaagtgg tcgccgaagc gggcgacggc
    18001 gcgagcgcga tcgatctggc gaatagactg gatatcgacg tgatcttgct ggatctcaat
    18061 atgaaaggta tgagtggcct ggataccctc aatgccttgc gcagggatgg cgttaccgcg
    18121 caaattatta tcctgaccgt atccgatgcc tccagcgatg tctttgcgct gatagacgca
    18181 ggcgcagacg gttatctgtt gaaagacagc gacccggaag tgttgcttga agcgattcgt
    18241 gccggagcga aaggcagcaa agtttttagc gaacgcgtca atcagtactt acgtgaacgt
    18301 gaaatgtttg gcgcggaaga agatcccttc agcgtgctga cggagcgcga gctggatgtt
    18361 ctgcacgagc tggcacaggg gctgtcaaat aaacagattg cctcggtgtt gaatatttcc
    18421 gagcagacag taaaagtaca cattcgcaat ctgctgcgta aactcaatgt ccgctcacgc
    18481 gtggcggcca ccattctgtt cctgcaacaa cgcggggcac aataaaaata gcccgatggc
    18541 atcaccatcg ggccactttt tatttactct cctgcggcga caaatgttgc atcgcctgcg
    18601 cgatactacg ttcgatcacc gcacggcgag tatcgttggc aggtaagagt ttcaacatca
    18661 tttcccacgc ggcaacggct tcgccaaatc gttgctgctc aaaggcatta aatgcataca
    18721 tgcttaacac acggatattg ctatggtccg ttctcaccaa ctggcgcagc agttcgccgc
    18781 cgaggcggtt gtcgttggga tcagatgaac gtgtcaacgc ttcagcgtat ccgagcgccg
    18841 catcactgtt cttcggatcg aggcgatatg cagtagcgta tgcatcggtg gcgatactgg
    18901 cgttacccag cgccatgcca acgcggccca acataatcca gccttctata tctcccggat
    18961 ttttttgcag ttgagtacgc atccccagcg caaggcgcga catctcttct tcgttgagcg
    19021 gagcggcttt cggatccagc gccctgtcca gtaacgccgg agcctgcgcg gtggcctgct
    19081 gccagatttt cacctgctga taattgccag tctggtagta gctgacgcca gccacaatta
    19141 acgccaccac aatacccggc agataaacaa catatccggc acgcttacct tccggcacgc
    19201 tttgttctgg aaacgcttcc ggcaccacgc gtacccgacg ccgcgaacgg gcgtatatga
    19261 cccaaccgcc aatgccaata gccactactg gcagcaccca cagcagcacg gtcagcggcg
    19321 ttaacggcgg atcgtaagtg acgaagttgc cgtaacgcgc caccatataa tcgacaatct
    19381 ctttcttact tttgccttcc tgcatcagtt catacacttt ctggcgcagg tcggtggcaa
    19441 tcatcgagtt ggaatcggca atgctgttgt tctggcattt cgggcagcgc agttcttcag
    19501 tgagctgacg gaactgttgt tcctgtgctt catctttaaa ctgcaacacg tcgatggtcg
    19561 ccagcgctga gccggagatc atcagcatca gcacgcccaa taaaaacctc attgtgcggc
    19621 ctccttactg tatttctccc acagtggctt gatctcttct tcccagacgc gaggattgag
    19681 atcgcccgca tggcgatagc gaatgatgcc gttgccgtca ataaggaacg tttctggcgc
    19741 gccatagaca ccgagatcca gccctaacat gccgtcgcca tcaaacaggc ttagtgcgta
    19801 aggattgccc aactctttca gccagctgat tgccttctgg cgatcgtctt tatagttcat
    19861 gccgaccacg cggatgccct gcgcagaaag ctgattcaga tattgatgtt ccgcacggca
    19921 ggtcggacac caggtcgccc agacgttaag cagcaccggt ttgccctgag tcagcacatc
    19981 cgcctgataa aactgccccg gattgtccag tgattcgagg cgaaacttcg gcactagctt
    20041 gccaatgagc gccgattcca gattggtcgg atcatcccct tcggcattac gcgccagctg
    20101 ccacagtagc gccgcggcaa tcgccaggaa gataatcaac ggaattaaca atactttgcg
    20161 cttcatacag cctccggcgc agttttctgc gggttcacgc gcttacgata gcgaggatca
//
LOCUS       DAZFLD010000079        19822 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.079, whole genome shotgun sequence.
ACCESSION   DAZFLD010000079 DAZFLD010000000
VERSION     DAZFLD010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19822
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.079"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(7..720)
                     /gene="lpxT"
                     /locus_tag="ACHKBL_002420"
     CDS             complement(7..720)
                     /gene="lpxT"
                     /locus_tag="ACHKBL_002420"
                     /EC_number="2.7.4.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo(2)-lipid A phosphotransferase"
                     /protein_id="HFO5819279.1"
                     /translation="MIKNLPQIVLLNIVGLALFLSWYIPVNHGFWLPIDADIFYFFNQ
                     KLVESKAFLWLVALTNNRAFDGCSLLAMGMLMLSFWLKENAPGRRRIVIIGLVMLLTA
                     VVLNQLGQALIPVKRASPTLTFTDINRVSELLSVPTKDASRDSFPGDHGMMLLIFSAF
                     MWRYFGKVAGLIALIIFVVFAFPRVMIGAHWFTDIIVGSMTVILIGLPWVLLTPLSDR
                     LITFFDKSLPGKNKHFQNK"
     gene            complement(759..1745)
                     /gene="yeiR"
                     /locus_tag="ACHKBL_002421"
     CDS             complement(759..1745)
                     /gene="yeiR"
                     /locus_tag="ACHKBL_002421"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding GTPase YeiR"
                     /protein_id="HFO5819280.1"
                     /translation="MTRTNLITGFLGSGKTTSILHLLAHKDPNEKWAVLVNEFGEVGI
                     DGALLADSGALLKEIPGGCMCCVNGLPMQVGLNTLLRQGKPDRLLIEPTGLGHPKQIL
                     DLLTAPVYEPWIDLRATLCILDPRLLLDEKSASNENFRDQLAAADIIVANKSDRATPE
                     SEQALQRWWQQNGGDRQLIHSEHGKIDGHLLDLPRRNLAELPASAAHSHQHGVKKGLA
                     ALSLPEHQRWRRSLNSGQGYQACGWIFDADTVFDTIGILEWARLAPVERVKGVMRIPE
                     GLVRINRQGDDLHIETQNVAPPDSRIELISSSEADWNALQSALLKLRLATTA"
     gene            complement(1863..3329)
                     /locus_tag="ACHKBL_002422"
     CDS             complement(1863..3329)
                     /locus_tag="ACHKBL_002422"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFO5819281.1"
                     /translation="MNTIASVTLPHHVHAPRYDRQQLQSRIVHFGFGAFHRAHQALLT
                     DRVLNAQSGDWGICEISLFSGDQLMSQLRAQNHLYTVLEKGADGNQAIIVGAVHECLN
                     AKLDSLAAIIEKFCEPQVAIVSLTITEKGYCIDPATGALDTSNPRIIHDLQTPEEPHS
                     APGILVEALKRRRERGLTPFTVLSCDNIPDNGHVVKNAVLGMAEKRSPELAGWIKEHV
                     SFPGTMVDRIVPAATDESLAEISQHLGVNDPCAISCEPFIQWVVEDNFVAGRPAWEVA
                     GVQMVNDVLPWEEMKLRMLNGSHSFLAYLGYLSGFAHISDCMQDRAFRHAARALMLDE
                     QAPTLRIKDVDLTQYADKLIARFANPALKHKTWQIAMDGSQKLPQRMLAGIRIHLARE
                     TDWSLLALGVAGWMRYVSGVDDAGNTIDVRDPLSDKIRDLVAGSSSEQRVTALLSLRE
                     VFGDDLPDNPHFVQAIEQAWQQIAQFGAHQALLNTLKI"
     gene            complement(3552..4124)
                     /gene="yeiP"
                     /locus_tag="ACHKBL_002423"
     CDS             complement(3552..4124)
                     /gene="yeiP"
                     /locus_tag="ACHKBL_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA];
                     GO_process: GO:0043043 - peptide biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HFO5819282.1"
                     /translation="MPRANEIKKGMVLNYNGKLLLVKDIDIQSPTARGAATLYKMRFS
                     DVRTGLKVEERFKGDDIVDTVTLTRRYVDFSYVDGNEYVFMDKEDYTPYTFTKDQIEE
                     ELLFMPEGGMPDMQVLTWDGQLLALELPQTVDLEIVETAPGIKGASASARNKPATLST
                     GLVIQVPEYLSPGEKIRIHIEERRYMGRAD"
     gene            4279..4533
                     /locus_tag="ACHKBL_002424"
     CDS             4279..4533
                     /locus_tag="ACHKBL_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HFO5819283.1"
                     /translation="MECRPGCGACCTAPSISSPIPGMPDGKPANTPCIQLDEQQRCKI
                     FTSPLRPKVCAGLQASAEMCGNSRQQAMTWLIDLEMLTAP"
     gene            complement(4530..5711)
                     /gene="setB"
                     /locus_tag="ACHKBL_002425"
     CDS             complement(4530..5711)
                     /gene="setB"
                     /locus_tag="ACHKBL_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetB"
                     /protein_id="HFO5819284.1"
                     /translation="MHNSPAVTSAKSFDLTSTAFLIVAFLTGIAGALQTPTLSIFLTD
                     EVHARPAMVGFFFTGSAVIGILVSQFLAGRSDKRGDRKSLIVFCCLLGVLACTLFAWN
                     RNYFVLLFVGVFLSSFGSTANPQMFALAREHADKTGREAVMFSSFLRAQVSLAWVIGP
                     PLAYALAMGFSFTVMYLSAAVAFIVCGVTVWLFLPSMQKELPLATGTVEAPRRNRRDT
                     LLLFVICTLMWGSNSLYIINMPLFIINELHLPEKLAGVMMGTAAGLEIPTMLIAGYFA
                     KRLGKRFLMRVAAVGGVCFYAGMLMAHSPVILLGLQLLNAIFIGILGGIGMLYFQDLM
                     PGQAGSATTLYTNTSRVGWIIAGSVAGIVAEIWNYHAVFWFAMVMIIATLFCLLRIKD
                     V"
     gene            6079..7209
                     /gene="fruB"
                     /locus_tag="ACHKBL_002426"
     CDS             6079..7209
                     /gene="fruB"
                     /locus_tag="ACHKBL_002426"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HFO5819285.1"
                     /translation="MFQLSVQDIHPGEKAGDKEEAIRQVAAALVQAGNVAEGYVNGML
                     AREQQTSTFLGNGIAIPHGTTDTRDQVLKTGVQVFQFPEGVTWGDGQVAYVAIGIAAS
                     SDEHLGLLRQLTHVLSDDSVAEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIV
                     ASDLLTLQALNAARLKEAGAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVS
                     RAANAFDVDGETAAMLVSVAMNDDQPIAVLKRLADLLLDNKADRLLKADAATLLALLT
                     SDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSL
                     MKVVALGVKKGHRLRFTAQGADAEQALKAIGDAIAAGLGEGA"
     gene            7209..8147
                     /gene="fruK"
                     /locus_tag="ACHKBL_002427"
     CDS             7209..8147
                     /gene="fruK"
                     /locus_tag="ACHKBL_002427"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFO5819286.1"
                     /translation="MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGIN
                     VAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGE
                     VTDFNFSGFDVTPADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQ
                     CPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIA
                     HVVISLGAEGALWVNASGEWIAKPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLR
                     LATAVAALAVSQSNVGITDRPQLAAMMARVDLQPFN"
     gene            8164..9855
                     /gene="fruA"
                     /locus_tag="ACHKBL_002428"
     CDS             8164..9855
                     /gene="fruA"
                     /locus_tag="ACHKBL_002428"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIBC"
                     /protein_id="HFO5819287.1"
                     /translation="MKTLLIIDANLGQARAYMAKTLLGAAARKAKLEIIDNPNDAEMA
                     IVLGDSIPNDSALNGKNVWLGDISRAVAHPELFLSEAKGHAKPYTAPVAATAPVATSG
                     PKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAAD
                     LVIVAADIEVDLAKFAGKPMYRTSTGLALKKTAQELDKAVAEATPYEPAGKAQTATTE
                     GKKEGAGAYRHLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGS
                     AFALMVPVLAGYISFSIADRPGLTPGLIGGMLAVSTGSGFIGGIIAGFLAGYIAKLIS
                     TQLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILEGLTHWLQTMGTANAV
                     LLGAILGGMMCTDMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPPLAMGLATMV
                     ARRKFDKAQQEGGKAALVLGLCFISEGAIPFAARDPMRVLPCCIVGGALTGAISMAIG
                     AKLMAPHGGLFVLLIPGAITPVLGYLVAIIAGTLVAGLAYAFLKRPEVDAVAKAA"
     gene            10279..11220
                     /locus_tag="ACHKBL_002429"
     CDS             10279..11220
                     /locus_tag="ACHKBL_002429"
                     /EC_number="2.7.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine kinase"
                     /protein_id="HFO5819288.1"
                     /translation="MREKDYVVIIGSANIDVAGYSHESLNYADSNPGKIKFTPGGVGR
                     NIAQNLALLGNKAWLLSAVGSDFYGQSLLTQTNQSGVYVDKCLIVPGENTSSYLSLLD
                     NTGEMLVAINDMNISNAITAEYLAQHGEFIQRAKVIVADCNISEEALAWILDNAANVP
                     VFVDPVSAWKCVKVRDRLNQIHTLKPNRLEAETLSGIALSGREDVAKVAAWFHQHGLN
                     RLVLSMGGDGVYYSDISGESGWSAPIKTNVINVTGAGDAMMAGLASCWVDGMPFAESV
                     RFAQGCSSMALSCEYTNNPDLSIANVISLVENAECLN"
     gene            11208..12146
                     /gene="psuG"
                     /locus_tag="ACHKBL_002430"
     CDS             11208..12146
                     /gene="psuG"
                     /locus_tag="ACHKBL_002430"
                     /EC_number="4.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HFO5819289.1"
                     /translation="MSELKISPELLQISPEVQDALKNKKPVVALESTIISHGMPFPQN
                     AQTAIEVEETIRKQGAVPATIAIIGGVMKVGLSKEEIELLGREGHNVTKVSRRDLPFV
                     VAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQELAHTNVTV
                     VCAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSPFDVSIRLDSASEIARA
                     MAVKWQSGLNGGLVVANPIPEQFAMPEHTINAAIDQAVAEAEAQGVIGKESTPFLLAR
                     VAELTGGDSLKSNIQLVFNNAILASEIAKEYQRLAG"
     gene            12240..13490
                     /locus_tag="ACHKBL_002431"
     CDS             12240..13490
                     /locus_tag="ACHKBL_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:1901642 - nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HFO5819290.1"
                     /translation="MDIMRSVVGMVVLLAIAFLLSVNKKSISLRTVGAALLLQIAIGG
                     IMLYFPPGKWAVEQAALGVHKVMSYSDAGSAFIFGSLVGPKMDVLFDGAGFIFAFRVL
                     PAIIFVTALISLLYYIGVMGLLIRILGSIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRMNRNELFTAICSGMASIAGSMMIGYAGMGVPIDYLLAASLMAIPGGILFAR
                     ILSPATEPSQVTFENLSFSETPPKSFIEAAASGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGIGGWFGFANASLESIFGYVLAPLAWIMGVDWSDANLAGSLIGQKLAINEFVA
                     YLSFSPYLQTGGTLEVKTIAIISFALCGFANFGSIGVVVGAFSAISPKRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(13558..14256)
                     /locus_tag="ACHKBL_002432"
     CDS             complement(13558..14256)
                     /locus_tag="ACHKBL_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311082.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator YeiL"
                     /protein_id="HFO5819291.1"
                     /translation="MKEIHNNDLKQQLMSESAFKDCFSTDVSADTRLFHFLARDYIVQ
                     EGQQPSWLFYLTRGRARLYATLANGRVSLIDFFAAPCFIGEIELIDKDHEPRAVQAIE
                     ECWCLALPMKHYRPLLLNDTLFLRKLCVTLSHKNYRNIVSLTQNQSFPLVNRLAAFIL
                     LSQEGDLYHEKHTQAAEYLGVSYRHLLYVLAQFIHDGLLIKSKKGYLIKNRKQLSGLA
                     LEMDPENKFSGMMQ"
     gene            14386..15327
                     /gene="rihB"
                     /locus_tag="ACHKBL_002433"
     CDS             14386..15327
                     /gene="rihB"
                     /locus_tag="ACHKBL_002433"
                     /EC_number="3.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosylpyrimidine nucleosidase"
                     /protein_id="HFO5819292.1"
                     /translation="MEKRKIILDCDPGHDDAIAIMMAAKHPAIDLLGITIVAGNQTLD
                     KTLINGLNVCQKLEINVPVYAGMPQPIMRQQIVADNIHGETGLDGPVFEPLTRQAEST
                     HAVKYIIDTLMASDGDITLVPVGPLSNIAVAMRMQPAILPKIREIVLMGGAYGTGNFT
                     PSAEFNIFADPEAARVVFTSGVPLVMMGLDLTNQTVCTPDVIARMERAGGPAGELFSD
                     IMNFTLKTQFENYGLAGGPVHDATCIGYLINPDGIKTQEMYVEVDVNSGPCYGRTVCD
                     ELGVLGKPANTKVGITIDTDWFWGLVEECVRGYIKTH"
     gene            15427..16677
                     /locus_tag="ACHKBL_002434"
     CDS             15427..16677
                     /locus_tag="ACHKBL_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:1901642 - nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HFO5819293.1"
                     /translation="MDVMRSVLGMVVLLAIAFLLSVNKKKISLRTVGAALVLQVVIGG
                     IMLWLPPGRWVAEKVAFGVHKVMAYSDAGSAFIFGSLVGPKMDTLFDGAGFIFGFRVL
                     PAIIFVTALVSILYYIGVMGILIRILGGIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKSFINRLNRNELFTAICSGMASIAGSTMIGYAALGVPVEYLLAASLMAIPGGILFAR
                     LLSPATESSQVSFNNLSFTETPPKSIIEAAATGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFEHASLESILGYLLAPLAWVMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTAGTLDAKTVAIISFALCGFANFGSIGVVVGAFSAVAPHRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(16733..17821)
                     /locus_tag="ACHKBL_002435"
     CDS             complement(16733..17821)
                     /locus_tag="ACHKBL_002435"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HFO5819294.1"
                     /translation="MNNREKEILAILRRNPLIQQNEIADMLQISRSRVAAHIMDLMRK
                     GRIKGKGYILTEQEYCVVVGTINMDIRGMADIRYPQAASHPGTIHCSAGGVGRNIAHN
                     LALLGRDVHLLSVIGDDFYGEMLLEETRRAGVNVSGCVRLHGQSTSTYLAIANRDDQT
                     VLAINDTHLLDQLTPQLLNGSRDLLRHAGVVLADCNLTAEALEWVFTLADEIPVFVDT
                     VSEFKAGKIKHWLAHIHTLKPTLPELEILWGQAITSDADRNAAVNALHQQGVQQLFVY
                     LPDESVYCSEKDGEQFLLTAPAHTTVDSFGADDGFMAGLVYSFLEGNNFRDSARFAMA
                     CAAISRASGSLNNPTLSADNALSLVPMV"
     gene            complement(17824..18681)
                     /gene="nfo"
                     /locus_tag="ACHKBL_002436"
     CDS             complement(17824..18681)
                     /gene="nfo"
                     /locus_tag="ACHKBL_002436"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HFO5819295.1"
                     /translation="MKYIGAHVSAAGGLANAAIRAAEIDATAFALFTKNQRQWRAAPL
                     TTQTIDEFKAACEKYHYTSAQILPHDSYLINLGHPVAEALEKSRDAFIDEMQRCEQLG
                     LSLLNFHPGSHLMQISEEDCLARIAESINIALDKTQGVTAVIENTAGQGSNLGFKFEH
                     LAAIIDGVEDKSRVGVCIDTCHAFAAGYDLRTSAECEKTFADFARIVGFKYLRGMHLN
                     DAKSTFGSRVDRHHSLGEGNIGHDAFRWIMQDDRFDGIPLILETINPDIWAEEIAWLK
                     AQQTEKAVA"
     gene            complement(18755..19804)
                     /locus_tag="ACHKBL_002437"
     CDS             complement(18755..19804)
                     /locus_tag="ACHKBL_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HFO5819296.1"
                     /translation="MTNITLQKQHRTLWHFIPGLALSAVITGVALWGGSIPAVAGAGF
                     SALTLAILLGMVLGNTIYPHIWKSCDGGVLFAKQYLLRLGIILYGFRLTFSQIADVGI
                     SGIIIDVLTLSSTFLLACFLGQKVFGLDKHTSWLIGAGSSICGAAAVLATEPVVKAEA
                     SKVTVAVATVVIFGTVAIFLYPAIYPLMSQWFSPETFGIYIGSTVHEVAQVVAAGHAI
                     SPDAENAAVISKMLRVMMLAPFLILLAARVKQLSGANSGEKSKITIPWFAILFIVVAI
                     FNSFHLLPQSVVNMLVTLDTFLLAMAMAALGLTTHVSALKKAGAKPLLMALVLFAWLI
                     VGGGAINYVIQSVIA"
BASE COUNT         4890 a         4877 c         5221 g         4834 t
ORIGIN      
        1 tgtgagttat ttgttttgga aatgtttgtt ttttcccggt agtgatttgt caaaaaaggt
       61 gattaatcga tcacttaatg gcgtcagcaa cacccagggc aaaccgatca atatcacggt
      121 catcgaaccg acaatgatgt cagtaaacca gtgtgcgcca atcattactc tgggaaacgc
      181 aaaaaccaca aaaataataa gggcgataag gcctgcaact ttgccgaaat aacgccacat
      241 gaatgccgaa aaaataagca gcatcatacc gtgatcgccg ggaaagctat ctcgtgaggc
      301 atctttcgtg ggaacagaga gcagttcgct gacgcggtta atatcggtaa aagtcaatgt
      361 tgggctggcc cgttttacag gaattaatgc ctgacccagc tggtttaata ccactgcagt
      421 taatagcatg accagaccaa taatcacgat acgtcgtctg cctggggcgt tttctttcag
      481 ccagaaactc agcatcaaca ttcccatcgc cagcagtgaa caaccgtcga aagcgcgatt
      541 gttggtcaat gcaaccagcc acaaaaaggc cttactttcg accagtttct gattaaaaaa
      601 ataaaaaata tccgcatcaa tcggcaacca gaatccatga ttaacgggaa tataccagga
      661 aagaaacagc gcgaggccga caatattcaa caacactatt tgcggcaaat ttttaatcat
      721 attttctcta attatcttac tgaaaaacgc aggcaacctt acgcggtggt cgctaaacga
      781 agcttcaaca gcgcgctctg caaggcattc cagtcagctt cgctgctgga aatcagctca
      841 atacgactgt ccggtggcgc aacgttttgc gtttcaatgt gcaggtcatc gccctgacgg
      901 ttgattcgca ccagcccttc gggaatacgc atcacgcctt tgacgcgttc caccggtgca
      961 agtcgcgccc attccagaat gccaatggtg tcgaataccg tatcagcgtc gaatatccag
     1021 ccacaggcct gatatccttg cccgctgttc agactgcgac gccagcgttg atgctctggc
     1081 aggcttaacg ctgctaaccc ttttttcacg ccatgctgat gagaatgcgc ggcgctggcg
     1141 ggcaactcgg ctaaattgcg acgcggcaaa tccagaagat gaccgtcaat tttcccatgc
     1201 tcgctgtgaa ttaattgccg atcgccacca ttttgctgcc accagcgctg tagcgcttgc
     1261 tcactttcgg gcgtcgcacg gtcggattta ttggcgacaa taatgtctgc ggcagccagc
     1321 tggtcacgga agttttcatt gctggcgctt ttttcgtcca gcagcaggcg tggatcgaga
     1381 atgcacaacg tggcgcgcag atctatccac ggttcataca cgggcgcggt taagagatct
     1441 aggatctgtt tcggatgacc cagtccggtc ggctctatca acaagcggtc tggttttccc
     1501 tgacgcagta aggtattcaa tcctacctgc atgggtaaac cattaacgca gcacatgcag
     1561 ccgccgggga tctctttcag caatgcgccg ctatcggcga gcaaagcacc atcaattccg
     1621 acttccccaa attcattaac cagtaccgcc catttttcgt tgggatcttt atgggctaac
     1681 agatgaagaa tcgacgtggt tttcccgctg ccgagaaaac cggtgatgag gttggtcctg
     1741 gtcacaacgg ctccgcaata ctcttaaaag ttaactgagc gttaatcctg gctaaaaacg
     1801 gcagaaaaga gaaggggaga gcggacgaag gcgagcttca tccgctttaa ccgcagaaat
     1861 cgttaaattt tgagggtgtt taatagcgcc tgatgcgcgc cgaattgtgc gatttgttgc
     1921 caggcttgtt cgatggcctg cacaaaatgc gggttatctg gcagatcatc accgaaaact
     1981 tcacgcaggg aaagcagggc ggtgacgcgt tgctcactgc tgctgcccgc aacaagatcg
     2041 cgaattttat cgctaagcgg atcgcgaaca tcaatggtat ttccggcatc atcaacgccg
     2101 ctgacgtaac gcatccagcc tgcaacgcct aatgccagca acgaccagtc cgtttcgcgc
     2161 gccagatgta tgcgaatacc tgccagcata cgttgcggta atttctggct accatccatt
     2221 gcgatttgcc aggtcttatg tttcagcgcc gggttagcaa aacgtgcaat taacttatcc
     2281 gcatattgtg ttaaatcgac atctttaatt cgcagtgtcg gcgcttgctc atccagcatt
     2341 aatgctctgg cggcatggcg aaatgcgcga tcctgcatgc aatcactgat atgggcgaat
     2401 cctgagaggt aacccagata agcgagaaaa gagtggctgc cattaagcat acgcagtttc
     2461 atctcttccc atggcaggac atcattcacc atttgtacac ctgcgacttc ccaggcagga
     2521 cgcccagcga cgaagttatc ttccaccacc cactggataa acggttcgca gctaatcgcg
     2581 cagggatcat ttacccccag atgctggctg atttccgcca gtgattcgtc ggttgctgcc
     2641 ggaacaatgc ggtcgaccat agttcccgga aaactgacgt gctcttttat ccacccggcg
     2701 agttctggcg aacgtttttc tgccattccc agcaccgcgt ttttcaccac atggccattg
     2761 tcgggaatat tgtcgcagga gagcacggta aacggtgtaa ggccgcgctc acggcggcgt
     2821 ttcagtgctt cgacgagaat acccggtgcg gagtgaggtt cttcaggggt ttgtagatcg
     2881 tgaataatcc gcggattgct ggtgtcgagt gcaccggtgg ccgggtcaat acaatagcct
     2941 ttttcggtaa tcgtcaggga aacaattgcc acctgtggct cgcaaaattt ctcaataatt
     3001 gccgctaagg aatccagttt tgcattcagg cattcgtgaa cggcaccgac aattatcgcc
     3061 tgattgccgt ccgccccttt ctccagcacg gtgtataaat ggttctgtgc gcggagctgg
     3121 ctcatcagtt gatcaccgct gaacaagctg atttcacaga tcccccagtc gccgctctgg
     3181 gcattcagca cacgatcggt cagtaacgcc tgatgtgcgc ggtgaaaggc tccaaagcca
     3241 aaatgaacga tacgtgattg caactgctgg cgatcgtagc gtggagcgtg tacatgatgc
     3301 gggagcgtaa cggaggcgat tgtattcata ggagtcacgt cttagtaacg attaattaac
     3361 aatgacagtg taaagttata tgacagcaat tgaaattggt ctggattgtt aatgcgatga
     3421 agtgtgaggg cgatcaatca acgagtgaaa attgcgggcg taaggtgttc aggaagcgca
     3481 aaatagaaga ttggtataat aaattaaaaa agggacagcc tgagctatcc cttttctgca
     3541 tgcggctgaa gttagtcagc acgccccata taacggcgtt cttcgatatg gatacgaatt
     3601 ttttcgcccg ggcttaagta ttctggtacc tgaatcacca gaccagtgct caatgtcgcc
     3661 ggtttgttac gggcgctggc agatgcccct ttgatgcctg gtgcggtttc aacgatttcc
     3721 agatcaacgg tctgcggcaa ctcaagcgcc agcagttgac cgtcccaggt cagcacctgc
     3781 atgtccggca tgccgccctc tggcataaac agcaactctt cttcaatctg atctttggtg
     3841 aaggtatacg gggtatagtc ttctttatcc ataaagacat attcgttgcc atcgacatag
     3901 gagaagtcaa cgtaacggcg ggtcagcgtc acggtgtcaa cgatatcatc tcctttgaaa
     3961 cgctcttcta ctttcagccc ggtacggaca tcagaaaaac gcattttgta cagcgtagcg
     4021 gcaccgcggg cagtgggcga ctgaatatca atgtccttaa ccagcagcag tttgccgttg
     4081 taattcagta ccataccttt tttaatttcg ttcgctcttg gcatcgaaaa attcctgtag
     4141 tcgaaaatgt caaaaatatc gcgacaaagt actcgcgacg ggcctttcgg gcaagtggta
     4201 ttcgcacttt tgctggtgca aaaaaggtgg tactgtgcgc gctcatcaat ccggtggtta
     4261 accttaagag aacaacgtat ggaatgccgt ccgggttgcg gggcgtgttg cactgcccct
     4321 tcgatttcca gccctattcc cggtatgcca gatggcaagc ccgccaatac gccctgcatt
     4381 cagcttgatg aacagcaacg ctgtaaaatt ttcacctcgc cactgcggcc gaaagtctgt
     4441 gcaggcttac aagccagcgc tgaaatgtgc ggtaactcgc gtcagcaggc aatgacctgg
     4501 cttatcgatc tggaaatgct gaccgcgcct taaacatctt taatccgcag taagcaaaac
     4561 agagtggcga taatcatcac catcgcaaac cagaacacag cgtgataatt ccagatctcg
     4621 gcgacgatgc ccgccactga tcctgcgatg atccagccca cgcgcgacgt attggtatag
     4681 agcgtggtgg ctgaacctgc ctgaccgggc atcaaatcct gaaaatagag catcccgatg
     4741 ccacccagaa tgccaataaa aatagcattt agcagctgca agcccaacag aatgacaggt
     4801 gaatgcgcca tcagcattcc tgcgtaaaaa cagacgccac ccacggcagc aacgcgcatt
     4861 aagaaacgct tacccagacg tttggcgaaa tatccggcaa tcaacatggt cgggatttcc
     4921 agcccggcgg cggtccccat catcacaccg gccagtttct cgggaagatg cagttcgttg
     4981 ataataaata gcggcatgtt gatgatgtac aggctgttcg agccccacat caatgtacaa
     5041 atgacaaaca gcagcagcgt atcgcgacgg ttacggcgcg gcgcttcaac cgtgccggtc
     5101 gccagcggaa gttctttttg cattgacggt aaaaacagcc acaccgtcac gccgcaaaca
     5161 ataaacgcta ccgctgcgct cagatacatt accgtaaagc tgaaacccat cgctaaggca
     5221 taagccagcg gtgggccaat gacccatgcc agtgaaacct gagcgcgtaa aaaagagctg
     5281 aacatcaccg cctcacgtcc ggttttgtcg gcatgttcac gggcaagggc aaacatttgc
     5341 gggttagcgg tcgagccaaa gctgctaaga aagacgccaa cgaatagcaa aacaaagtag
     5401 ttgcgattcc aggcaaaaag ggtgcaggcc agcacgccta acaggcagca aaagacaatc
     5461 agcgatttgc gatcgccgcg cttatcagag cgcccggcga gaaactgact caccagaatc
     5521 ccaatgacag cgctgccggt aaagaagaat cccaccatcg ccggacgggc atgtacttca
     5581 tcggtaagaa aaatactgag tgtcggggtt tgcagagcgc ccgcaatacc ggtgagaaag
     5641 gcaacgatta aaaacgccgt cgaggtcagg tcaaacgatt tcgcgctggt gactgcgggg
     5701 gagttatgca tgctggtcga tcactggaag aggaagacgc ggagtttacg ctgcttgtca
     5761 gaaaataaac agacagcgaa atcaaaagcg ccacgaataa tcaaaatgcc atttcactgt
     5821 tgctgattgg ctgctgacgc tgctgaaatg aaattgctga taacggattt tcccatcagc
     5881 aattaggaaa aatgacaaaa aattgtgcag cacatcaaac ttttgctcat aactttacgg
     5941 ctttccttgc gtgctgaaac aaaagtatta tgctctcttg aaacgtttca gcgcgatctt
     6001 gtctttaacc ctaagccagg ttggcgcttt ttttctcata gaagctgaat cgtttcaatt
     6061 cagcaagaga ggagaactat gttccagtta tccgtacagg acatccatcc gggcgaaaag
     6121 gccggagaca aagaagaggc gattcgccag gtcgctgcgg cgctggtgca ggccggtaat
     6181 gtagctgaag gctacgtcaa tggcatgctg gcgcgcgaac agcaaacctc aacgttcctc
     6241 ggcaatggta ttgctattcc acacggcact accgacaccc gcgatcaggt gctgaaaacc
     6301 ggcgttcagg tatttcagtt cccggaaggc gtcacctggg gtgacggcca ggtagcgtac
     6361 gtggcgatcg gtattgctgc cagctcggat gaacatctgg gcctgctacg ccagctgacc
     6421 cacgtactga gcgatgattc cgttgctgaa caactgaagt cagcaacaac agcagaagaa
     6481 cttcgcgcat tgctgatggg cgaaaagcag agtgagcagc tgaagctcga caacgaaatg
     6541 ctgacgctgg atatcgtcgc cagcgatctg ctgactcttc aggcgctgaa cgctgcgcgt
     6601 ctgaaagaag cgggggcagt tgacgccact ttcgtcacca aagccatcaa tgaacaaccg
     6661 ctgaacctcg ggcagggtat ctggctgagc gatagcgccg aaggcaatct gcgtagcgcg
     6721 attgcggtaa gccgtgcggc aaatgctttt gatgtggacg gcgaaacggc agccatgctg
     6781 gtgagtgtgg cgatgaatga cgatcagccc attgcggttc ttaagcgtct cgctgatttg
     6841 ttactcgaca ataaagctga ccgcttgctg aaagcggatg cggcaacgtt gctggcgctg
     6901 ctgaccagcg atgatgcgcc gaccgacgac gtgttaagcg cggagtttgt ggtgcgcaat
     6961 gaacacggcc tgcatgctcg tccaggtacc atgctggtca ataccattaa acaatttaac
     7021 agtgatatta ccgtgacaaa ccttgatggt accggcaaac cggcaaacgg acgtagtctg
     7081 atgaaagttg tggcacttgg cgttaagaaa ggtcatcgcc tacgctttac cgcccagggt
     7141 gcagatgctg aacaggcgct gaaagcaatc ggcgacgcta tcgctgctgg tcttggggag
     7201 ggcgcataat gagcagacgt gttgctacta tcacccttaa tccggcttat gaccttgttg
     7261 gtttctgccc ggaaattgaa cgcggcgaag tgaacctggt gaaaaccacc ggtctgcatg
     7321 cggcgggtaa aggcatcaac gtggccaaag tattaaaaga cctgggaatt gatgtcaccg
     7381 ttggtggctt cctcggtaaa gacaatcagg atggttttca gcaactgttc agcgagctgg
     7441 gcattgccaa ccgtttccag gttgtacagg ggcgcacccg aattaacgtt aagctgacgg
     7501 aaaaagacgg cgaagtgacc gacttcaact tctcgggttt tgacgttacc cccgccgact
     7561 gggaacgctt tgtgactgat tctctgagct ggctcggtca gttcgatatg gtctgtgtca
     7621 gcggaagctt accgtcaggc gtcagcccgg aagcgttcac cgactggatg actcgcctgc
     7681 gtagtcagtg tccttgcatt atctttgata gtagccgtga agcgttagta gcaggtttga
     7741 aagcggcacc gtggctggtg aaacctaacc gccgcgagct ggaaatctgg gcaggccgta
     7801 aactgcctga aatgaaagat gtgattgaag ctgcgcatgc gctgcgtgaa caaggtatcg
     7861 cgcatgtcgt tatttcactg ggtgccgaag gcgcgctttg ggttaatgcc tccggcgaat
     7921 ggatcgccaa accaccgtca gtcgatgtcg taagcaccgt tggcgcaggg gattctatgg
     7981 ttggtggcct gatttatggc ttgctgatgc gtgaatccag tgaacacaca ctgcgtctgg
     8041 cgacagctgt tgcagccctg gcggtaagtc aaagcaatgt gggtattacc gatcgtccgc
     8101 agttggccgc aatgatggcg cgcgtcgact tacaaccttt taactgacag caggagaggc
     8161 ataatgaaaa cgctgctgat tattgacgct aatctcggtc aggcacgcgc ctatatggcg
     8221 aagaccctgc tgggcgcggc ggcgcgaaaa gcaaaactgg aaatcatcga caatccgaac
     8281 gacgctgaaa tggcgattgt tctcggtgat tccatcccga acgacagcgc gctgaacggt
     8341 aaaaatgtct ggctgggcga tatttcccgg gcagttgcgc accctgagct gttcctgagt
     8401 gaagccaaag gccatgcgaa accttacact gcaccggtcg ctgcgacagc accagttgcc
     8461 accagcggtc cgaaacgcgt agttgcggtg acagcttgcc cgactggcgt agcacacaca
     8521 tttatggcgg ctgaagccat tgaaaccgaa gcgaaaaaac gtggctggtg ggtgaaagtt
     8581 gaaacccgtg gttctgttgg cgcgggtaat gcgatcaccc ctgaagaagt agccgcagcg
     8641 gatctggtga ttgtggcggc agatatcgaa gtggatctgg cgaaatttgc tggtaaaccg
     8701 atgtatcgca cctctaccgg tctggcgctg aagaaaaccg cgcaggaact ggataaagcg
     8761 gttgctgaag caacgccgta tgaaccggcg ggcaaagctc aaacagcgac cactgaaggt
     8821 aagaaagagg gtgcaggcgc ttaccgtcac ttgctgacgg gcgtctctta catgctgccg
     8881 atggtcgttg caggtggtct gtgtatcgcg ctttcttttg cttttggtat cgaagcgttt
     8941 aaagagccgg gtacgttggc agcggcgctg atgcagattg gtggtggttc agcctttgcg
     9001 ctgatggtgc cggtactggc aggttacatt tccttctcca ttgccgatcg tccgggtctc
     9061 acgccgggtc tgattggggg tatgctggcg gtcagcaccg gttctggctt cattgggggt
     9121 attattgcgg gcttcctggc tggttacatt gcgaagttaa tcagtacgca actgaaactg
     9181 ccacagagta tggaggcgct gaaaccgatc ctgatcattc cgctaatttc cagtctggtg
     9241 gtcggtctgg cgatgatcta cctgatcggt aaaccggttg ctggcattct cgaagggttg
     9301 actcactggc tgcagaccat ggggactgcg aatgcggttc tgctgggggc gatcctcggt
     9361 ggcatgatgt gtactgacat gggcggtccg gtaaataaag cagcgtacgc attcggtgtg
     9421 ggtctgctga gtactcaaac ctatggcccg atggcggcga ttatggcggc aggtatggtg
     9481 ccaccgctgg caatgggtct ggcaacaatg gtggcgcgtc gcaaattcga caaagcgcag
     9541 caggaaggtg gcaaagccgc tctggttctt ggtctgtgct ttatttcgga aggtgcaatt
     9601 ccgtttgctg ctcgtgatcc gatgcgtgtg ctgccgtgtt gtatcgttgg tggggcgctg
     9661 actggcgcaa tctcaatggc gattggtgcg aaacttatgg caccgcacgg tggtctgttt
     9721 gttctgctga tccctggcgc tattacgccg gtactgggtt acctggtagc aattattgcc
     9781 ggtacgctgg tggcgggttt ggcctatgcc ttcctgaaac gtccggaagt ggacgcagta
     9841 gcgaaagcag cgtaataaaa ggtgtgttac agggcagaaa tttcctgccc tgtaacattt
     9901 gatgagcgca aagggaatga ccagtttgtg aatgtaattt tatgaattta aatggtttta
     9961 tctttcagta gaaacctgaa atgttcatca aacactaagg ttatttcctc aagggcagat
    10021 tattttctgc ccttttttag tgttcctgtt ctgtgcatcc cgtcacaaat tcctcacttt
    10081 tgtaattcag gcatttaatc gcattttgtg agcctgctca aatctgccca ttttgcctct
    10141 ctggcggcaa tttactttac gtaaaacatg catactatga gcacatgttt aaatatgaac
    10201 acatgtttaa accgtctttg cagtatctat attcttgtgg attgacagga ttggtaattt
    10261 tttaccagag gacgtgctat gcgcgaaaag gattatgtcg taattatagg ttcggcgaat
    10321 attgatgtcg ccggatattc acatgaatca ttaaattatg cggattcaaa tccaggtaaa
    10381 ataaagttta cgcctggtgg agtagggcgc aatattgcac aaaacctggc gttgctgggg
    10441 aataaagcct ggctactgag cgccgtaggc agtgattttt atggtcaatc gctgctaacg
    10501 caaaccaatc aatctggtgt ttatgtcgat aaatgcctga ttgtgccggg agaaaatacg
    10561 tcgagctatt tatcattact cgataatacc ggtgaaatgc tggttgctat aaatgacatg
    10621 aatattagca acgctattac agctgaatat ctcgcacagc acggtgaatt tattcagagg
    10681 gcaaaggtca ttgtcgcgga ctgtaatatc agtgaagagg cactggcatg gattctggat
    10741 aatgccgcca acgtacccgt atttgtcgat cctgtttccg cgtggaaatg tgtcaaagtg
    10801 cgcgaccgtc taaatcaaat ccacactctc aagccaaacc gccttgaagc ggaaaccctg
    10861 agtgggattg ctttgtcagg gcgtgaagat gtggcaaaag ttgctgcctg gttccatcaa
    10921 catggcctga accgactggt attgagcatg ggcggcgacg gcgtttatta cagtgatatc
    10981 agcggtgaaa gtggttggtc tgcgccgatc aaaaccaatg tcattaatgt taccggagcg
    11041 ggcgatgcca tgatggcggg acttgcttcg tgttgggtag acggaatgcc gtttgccgaa
    11101 tctgttcgtt tcgcacaggg atgttcgtca atggcgctct cctgtgaata caccaataac
    11161 cccgatttat cgattgccaa cgttatatcg ttagtggaga acgcagaatg tctgaattaa
    11221 aaatttcccc tgaattatta caaatttccc cggaagtgca ggacgcttta aaaaacaaaa
    11281 aaccggttgt ggcgctggaa tcgaccatta tttctcacgg gatgccgttc ccacaaaatg
    11341 cccagaccgc aattgaagtt gaagaaacta ttcgtaaaca gggcgctgta cctgccacta
    11401 tcgccattat tggcggcgtg atgaaagtgg gtttaagcaa agaagaaatt gaattgctgg
    11461 gtcgtgaagg gcataacgtg accaaagtta gtcgtcgcga tttacctttt gttgttgccg
    11521 ccggaaaaaa tggtgcaacc accgttgctt caacgatgat tattgcggcg cttgccggaa
    11581 ttaaagtatt tgccaccggg ggaattggtg gtgtgcatcg cggggcggaa cataccttcg
    11641 atatttctgc cgatttgcaa gaactggcgc acaccaatgt caccgtcgtt tgtgccgggg
    11701 cgaaatctat tctcgattta ggcttaacta ccgaatattt agaaactttc ggtgtaccgc
    11761 taattggcta tcagaccaaa gcgctgcctg cgtttttctg ccgcaccagc ccgtttgacg
    11821 tgagcattcg tctcgacagc gccagcgaaa ttgcccgtgc aatggcggtg aaatggcaaa
    11881 gcgggctgaa cggtggcctc gtggtagcga acccgatccc ggaacagttt gctatgccgg
    11941 aacacactat caatgcggcg atcgatcagg cggtagctga agctgaagcg cagggtgtta
    12001 ttggtaaaga aagtacgcca ttcctgctgg cgcgcgttgc tgagttgacc ggcggtgaca
    12061 gcctgaaatc caacatccag ctggtgttca acaacgccat cctggcgagt gaaatagcca
    12121 aagaatacca gcgtctcgcg ggttaatttc tgacgggcag aaagccgtcg cccgtactca
    12181 tcaagcatac caaaacctgg cgctgacgcc gggtttactg gcatgaaggg aaaatcatta
    12241 tggatataat gagaagtgtt gtggggatgg tggtgttact ggcaatagca tttctgttgt
    12301 cagtgaataa aaagagcatc agtttgcgca cggttggagc cgcactgctg ctgcaaatcg
    12361 ctattggtgg catcatgctc tacttcccac cgggaaaatg ggcagtagaa caggcggcat
    12421 taggcgttca taaagtgatg tcttacagtg atgccggtag cgccttcatt tttggttcgc
    12481 tggtcgggcc gaaaatggat gtcctctttg acggtgcggg ttttatcttc gcctttcgcg
    12541 tacttccggc gattatcttc gtcactgcgc tcatcagtct gctgtactac attggcgtga
    12601 tggggctgct gattcgcatc cttggcagca ttttccagaa agccctcaac atcagcaaaa
    12661 tcgaatcttt tgttgcggtt accactattt tcctcgggca aaatgagatc ccggcgatcg
    12721 ttaaaccgtt tatcgatcgc atgaatcgca acgagttgtt taccgcaatt tgtagcggga
    12781 tggcgtccat tgctggttcg atgatgattg gttatgccgg aatgggcgta ccaattgact
    12841 acctgttagc ggcatcgctg atggcgatcc ctggtggtat tttgtttgca cgtattctta
    12901 gcccagccac cgagccttcg caggtgacat ttgaaaatct gtcgttcagc gaaacgccgc
    12961 caaaaagctt tatcgaagcg gcggcgagcg gtgcgatgac cgggctgaaa atcgccgctg
    13021 gtgtggcgac ggttgtaatg gcgtttgtcg caattattgc gctgatcaac ggcattatcg
    13081 gcggaattgg cggctggttt ggtttcgcca atgcctctct ggaaagtatt tttggctatg
    13141 tgctggcacc gctggcgtgg atcatgggtg tggactggag tgatgccaat cttgcgggta
    13201 gcctgattgg gcagaaactg gcgattaacg aattcgtcgc ttacctgagt ttctccccat
    13261 acctgcaaac gggcggcacg ctggaagtga aaaccattgc gattatctcc tttgcgcttt
    13321 gtggttttgc taactttggt tctatcggtg ttgtcgttgg ggcattttcg gctatttcgc
    13381 caaaacgcgc accggaaatc gcccagcttg gtttacgggc gctggcagca gcaacgcttt
    13441 ccaacctgat gagcgcgacc attgccgggt tctttattgg tttagcttga gtgtcattgc
    13501 cggatgtacg cgcttattaa gcgtcacatc cggcaatcgc tctattgcaa ataattttta
    13561 ctgcatcatc cctgagaatt tattttccgg gtccatctcc agtgccagtc ctgacaactg
    13621 ctttctgttt ttaatgagat accctttctt gcttttaatt aataaaccgt cgtgaatgaa
    13681 ttgcgcgaga acatataaaa gatgtcgata agaaacacct aaatactctg ccgcttgcgt
    13741 atgcttttcg tgataaagat caccttcctg cgagagtaat ataaaagctg ccaggcgatt
    13801 cactaacgga aatgattgat tctgagttaa agaaacaata ttacgataat ttttatgact
    13861 taaggtgacg cagagttttc gtaaaaatag cgtgtcgttt aataacagcg ggcggtaatg
    13921 tttcataggg agcgcaaggc accaacactc ttcaatagcc tgcaccgcac gcggttcatg
    13981 gtctttgtcg attaactcaa tctcgccaat aaaacaggga gcggcaaaga aatcgatcag
    14041 cgacacgcga ccattagcaa gcgtggcgta aagcctggca cggcctcgcg tcagataaaa
    14101 cagccatgac ggctgttgcc cttcctgcac aatgtagtca cgcgctaaaa aatgaaacag
    14161 ccgcgtatcg gctgaaacat ccgttgaaaa gcaatcctta aacgcagatt cactcatcaa
    14221 ttgctgctta aggtcattat tgtggatttc tttcacgatg tttatccgct atgagatttc
    14281 tcatatttag catacttacg ctgttgttaa gattcaatca tcggcccggt cgaattttct
    14341 ttgctgtaga aaaaataacg caactggaaa cagaggaaat aaacaatgga aaagagaaaa
    14401 attattctgg attgtgatcc gggtcatgat gatgctattg ccataatgat ggcagcgaaa
    14461 catccggcga ttgatttatt aggtatcacc attgtggcgg gtaatcagac gcttgataaa
    14521 acattaatta atggcctgaa tgtttgccag aaactggaga ttaatgttcc ggtttatgcg
    14581 gggatgccac agcccattat gcgtcaacaa atcgttgccg ataatattca cggtgaaacc
    14641 ggactggatg gcccggtatt cgagccgctg acccgccagg cagaaagcac tcatgcggtg
    14701 aaatatatca ttgataccct gatggcaagc gatggcgata tcactttggt gccggttggc
    14761 ccactttcaa atatcgcggt ggcaatgcgt atgcaacccg cgatcctgcc gaaaatccgt
    14821 gaaattgtgc tgatgggcgg cgcttacggt actggcaact tcaccccatc tgccgagttc
    14881 aacatctttg ctgacccgga agccgcacgc gtggtgttca cctccggcgt tccattagtg
    14941 atgatgggcc tcgatctcac caaccagacc gtttgcaccc cggacgtgat tgctcggatg
    15001 gaaagggcag gcggccctgc cggagagctg ttcagcgaca tcatgaactt cactctcaaa
    15061 acccagttcg aaaactacgg cctggctggc ggcccggtgc atgacgccac ctgcatcggt
    15121 tatctgatta accctgatgg cattaaaacc caggagatgt acgtcgaagt ggacgttaac
    15181 agcggccctt gctacgggcg tacggtctgc gacgagctgg gcgttcttgg caagcccgcc
    15241 aataccaaag tcggcatcac tattgatact gactggttct ggggattagt cgaagagtgc
    15301 gtgcgcggct acatcaaaac ccattaagtg ttgataacaa gccgggacag tatcgcaaga
    15361 tgcaggcccg gctatttctg atgggcagaa agccgtcgcc caaaatgaca tgaagagata
    15421 ataactatgg atgtcatgag aagtgttctg ggaatggtgg tattgctggc aattgcgttt
    15481 ttgctgtcag taaacaagaa gaagatcagc ctgcgtaccg ttggcgcggc gttagtgtta
    15541 caggtcgtga ttggcggcat tatgctttgg ttaccaccag ggcgttgggt cgcagaaaaa
    15601 gtcgcttttg gcgtgcataa agtgatggcg tacagcgatg caggtagcgc atttatcttt
    15661 ggttctctgg tcggaccaaa aatggatacc ttatttgatg gcgcaggatt tatctttggt
    15721 ttcagggtat taccggcaat tatcttcgtt acagcactgg tgagtattct ctactacatc
    15781 ggtgtgatgg ggatcttaat tcgcattctc ggcggtatct tccagaaagc attgaatatc
    15841 agcaagatcg agtcattcgt cgcggtcact accattttcc tcgggcaaaa cgaaattccg
    15901 gcaatcgtca aatcctttat caatcgtctg aatcgcaatg aattatttac agcgatttgt
    15961 agcgggatgg cctcgattgc tggttcgaca atgattggtt acgccgcact gggcgtgcct
    16021 gtggaatatc tgctggcggc atcgttaatg gcgatccccg gcgggatctt gtttgcccgc
    16081 ctgttaagcc cggcaacaga atcttcgcag gtttccttta ataacctctc tttcaccgaa
    16141 acaccgccaa aaagcattat tgaagccgct gcgacagggg caatgaccgg gctgaaaatc
    16201 gccgcaggtg tggcgacagt agtgatggca tttgtcgcca tcattgcgtt aattaacggt
    16261 attatcggcg gcgttggcgg ctggtttggc tttgaacatg cctcgctgga gtccatttta
    16321 ggttacctgc tggccccact ggcgtgggta atgggtgttg actggagtga tgcgaatctt
    16381 gccgggagtt tgattggaca gaaactggca ataaatgaat ttgtcgctta cctcaatttc
    16441 tcaccttatc tgcaaacagc tggcactctg gatgctaaaa ccgtggcgat tatttccttc
    16501 gcgttgtgcg gtttcgctaa ctttggttct atcggggtgg tggtgggggc gttttctgcg
    16561 gttgcgccac accgtgcacc ggaaatcgcc cagcttggtt tacgcgcgct ggcggcggcg
    16621 acactttcta acctgatgag tgcaaccatt gcaggattct ttattggctt agcgtagtgc
    16681 gcgagttatg agccggataa gatgtagtca agcgtcgaat ccggcaacat tgttacacca
    16741 tcggcactaa tgaaagcgcg ttatcggcag acagggtagg gttgtttaag ctgccgctgg
    16801 cgcgtgaaat tgccgcgcag gccatcgcaa aacgggcgct gtcacggaaa ttgtttccct
    16861 ccagaaagct atataccagg cccgccataa aaccatcgtc agcaccaaaa ctgtctactg
    16921 tcgtatgcgc tggcgctgtc agcaaaaatt gctctccatc cttttcgctg caataaactg
    16981 actcatcggg caaataaaca aacagttgct gaacgccttg ctgatgcaac gcattcactg
    17041 cggcattacg gtcagcatcg ctggtgatcg cctgtcccca taaaatttcc agctccggta
    17101 aagtgggttt cagggtgtga atatgcgcca gccagtgttt gattttgccc gctttgaatt
    17161 ctgaaacggt atcgacaaac accgggattt catcagcgag ggtaaagacc cattccagcg
    17221 cctcggctgt caggttacaa tcagcaagta ccacgcccgc atgacgaagt aaatcgcgcg
    17281 acccgttcag tagttgcggt gtcaactgat ccagcagatg ggtatcgtta atcgccagca
    17341 cggtttgatc gtctcgattg gcaattgcca gatacgtcga tgtgctttgg ccatgtaaac
    17401 gaacgcagcc ggagacattc acgccggcgc ggcgcgtttc ttccaggagc atttcgccat
    17461 aaaagtcatc accaatcact gaaagcaaat ggacgtcgcg gcctaacagc gccaggttgt
    17521 gggcgatatt gcgtccaacg ccgcctgccg agcaatgaat ggtaccggga tgagaagccg
    17581 cttgcgggta acggatatcc gccatcccgc gaatatccat attgattgtc cccaccacta
    17641 cgcagtattc ctgctcggtg agaatgtaac ctttgccttt aatccggcct ttgcgcatta
    17701 aatccataat atgcgccgca acgcgcgaac ggctgatttg cagcatatcc gcaatttcgt
    17761 tctgctgaat cagcgggtta cgccgtaaaa ttgcaaggat ctccttctcc cggttgttca
    17821 tcttcaggct accgcttttt cagtttgttg cgctttcagc caggcgatct cttctgccca
    17881 gatatccggg ttgatggttt caaggatcag cggaatgccg tcgaaacggt cgtcctgcat
    17941 gatccagcgg aacgcatcat gaccaatatt gccttcaccg aggctatgat ggcggtcaac
    18001 gcggctgcca aaggtgcttt tcgcatcgtt aaggtgcatc ccgcgcagat acttaaagcc
    18061 aacaatacgg gcaaaatccg cgaacgtttt ctcgcattcg gcagaagtac gcaaatcata
    18121 cccggcagca aaagcatggc aggtatcaat gcagacgccg acgcgggatt tatcttccac
    18181 gccgtcgata atcgccgcga gatgttcgaa tttaaaccct aagttactgc cctgaccggc
    18241 ggtgttttca atcaccgctg tcacaccttg agttttatcc agcgcaatgt tgatggattc
    18301 ggcaatacgc gcaaggcaat cctcttctga aatctgcatc agatggctgc cagggtggaa
    18361 gttgagcaaa gaaagcccca gctgttcgca acgctgcatt tcatctataa acgcatcgcg
    18421 cgatttttcc agagcttcag cgaccggatg tccgaggtta atcagataac tgtcgtgggg
    18481 aagaatttgc gccgatgtgt agtgatattt ttcacaggcg gctttgaatt catcgatggt
    18541 ttgcgtcgtg agcggtgcgg cacgccactg acgttggttt ttggtgaaca aggcaaacgc
    18601 ggttgcgtcg atttcggcgg cgcgaattgc ggcatttgcc agaccgccag cagcactaac
    18661 gtgcgctcca atgtatttca tgcgaggact cctgttaaac ccgctggagg aaaacggtaa
    18721 tgatagcggg ttaacaggag caagatgtag tggtttatgc gatgacgctt tgaatcacat
    18781 agttaatcgc accaccacca acaatcagcc aggcaaacag taccagtgcc atcagcagag
    18841 gtttcgcccc ggcttttttc agcgcgctga cgtgagtggt cagacccagc gccgccatcg
    18901 ccattgccag caggaaggta tccagcgtta ccagcatgtt caccacgctc tgcggtaaca
    18961 ggtggaacga gttaaagatg gcaactacga tgaacaagat ggcaaaccac ggaatagtga
    19021 ttttgctttt ctcgccgctg ttcgccccag acagctgttt aacacgcgcc gccagcagga
    19081 tgaggaacgg agccagcatc atcacgcgca gcattttgga aataactgct gcgttttctg
    19141 catccgggct gatggcatga cctgccgcca ccacctgcgc cacttcgtgc acagtagaac
    19201 caatgtagat accgaaagtt tccggactaa accattgaga catcagcgga tatatcgccg
    19261 ggtagaggaa aatcgcgacg gtcccgaaga taacaacggt tgcaacagcc acggttactt
    19321 tactggcttc cgctttcact accggctcag tcgccagtac cgcggcagca ccacagatac
    19381 tgctaccggc accgatcaac cagctggtgt gcttatccag accaaacact ttctgcccca
    19441 ggaagcaagc cagcaggaag gtactggaca gcgtcagcac gtcaatgatg atcccactga
    19501 taccgacatc ggcaatttgc gagaacgtca gacggaagcc ataaagaatg atacccagac
    19561 gtaataaata ttgcttggca aacagcacac caccgtcaca gcttttccag atgtgcggat
    19621 agatggtgtt gcctaaaacc atccccaaca agattgcgag ggtgagggca ctaaacccgg
    19681 cacccgcaac cgcgggaatg gaaccacccc acagggcgac cccggtgata actgcactca
    19741 gggctaaccc cggaataaaa tgccacagtg tacgatgttg tttctgtaag gtgatattcg
    19801 tcataaccct ctcctttaac cg
//
LOCUS       DAZFLD010000080        19730 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.080, whole genome shotgun sequence.
ACCESSION   DAZFLD010000080 DAZFLD010000000
VERSION     DAZFLD010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19730
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.080"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            1046..1366
                     /gene="clpS"
                     /locus_tag="ACHKBL_002438"
     CDS             1046..1366
                     /gene="clpS"
                     /locus_tag="ACHKBL_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HFO5819297.1"
                     /translation="MGKTNDWLDFDQLAEEKVRDALKPPSMYKVILVNDDYTPMEFVI
                     DVLQKFFSYDVERATQLMLAVHYQGKAICGVFTAEVAETKVAMVNKYARENEHPLLCT
                     LEKA"
     gene            1397..3673
                     /gene="clpA"
                     /locus_tag="ACHKBL_002439"
     CDS             1397..3673
                     /gene="clpA"
                     /locus_tag="ACHKBL_002439"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0043335 - protein unfolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HFO5819298.1"
                     /translation="MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPSAREAL
                     EACSVDLVALRQELEAFIEQTTPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRNE
                     VTGANVLVAIFSEQESQAAYLLRKHEVSRLDVVNFISHGTRKDEPTQSSDPGSQPNSE
                     EQAGGEERMENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGV
                     GKTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFEKRFKALLKQLEQ
                     DTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRVIGSTTYQEFSNIFEKD
                     RALARRFQKIDITEPSIEETVQIINGLKPKYEAHHDVRYTAKAVRAAVELAVKYINDR
                     HLPDKAIDVIDEAGARARLMPVSKRKKTVNVADIESVVARIARIPEKSVSQSDRDTLK
                     NLGDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQL
                     SKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPHAVLLLDEI
                     EKAHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIGLIHQDN
                     STDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVELQVQLDQKGVSLEV
                     SQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANELLFGSLVDGGQVTVALDKEKN
                     ELTYGFQSAQKHKAEAAH"
     gene            complement(4196..4283)
                     /locus_tag="ACHKBL_002440"
     tRNA            complement(4196..4283)
                     /locus_tag="ACHKBL_002440"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4247..4249),aa:Ser,seq:gga)
     gene            complement(4537..4755)
                     /gene="infA"
                     /locus_tag="ACHKBL_002441"
     CDS             complement(4537..4755)
                     /gene="infA"
                     /locus_tag="ACHKBL_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HFO5819299.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNY
                     IRILTGDKVTVELTPYDLSKGRIVFRSR"
     gene            complement(5040..5744)
                     /gene="aat"
                     /locus_tag="ACHKBL_002442"
     CDS             complement(5040..5744)
                     /gene="aat"
                     /locus_tag="ACHKBL_002442"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HFO5819300.1"
                     /translation="MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQR
                     GIFPWFSPGDPILWWSPDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQVIEGC
                     ASDREEGTWITRGVVEAYHRLHELGHAHSIEVWREDELVGGMYGVAQGTLFCGESMFS
                     RMENASKTALLVFCEEFIGHGGKLIDCQVLNDHTASLGACEIPRRDYLNYLNQMRLGR
                     LPNNFWVPRCLFSPQE"
     gene            complement(5786..7507)
                     /gene="cydC"
                     /locus_tag="ACHKBL_002443"
     CDS             complement(5786..7507)
                     /gene="cydC"
                     /locus_tag="ACHKBL_002443"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0034775 - glutathione transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HFO5819301.1"
                     /translation="MRALLPYLALYKRHKWMLSLGIVLAIVTLLASIGLLTLSGWFLS
                     ASAVAGVAGLYSFNYMLPAAGVRGAAITRTAGRYFERLVSHDATFRVLQHLRIYTFSK
                     LLPLSPAGLARYRQGELLNRVVADVDTLDHLYLRVISPLVGAFVVIMVVTIGLSFLDF
                     TLAFTLGGIMLLTLFLMPPLFYRAGKSTGQNLTHLRGQYRQQLTAWLQGQAELTIFGA
                     SDRYRTQLENTEIQWLEAQRRQSELTALSQAIMLLIGALAVILMLWMASGGVGGNAQP
                     GALIALFVFCALAALEALAPVTGAFQHLGQVIASAVRITDLTDQKPEVTFPDTQTRVA
                     DRVSLTLRDVQFNYPEQSQQALKGISLQVNAGEHIAILGRTGCGKSTLLQLLTRAWDP
                     QQGEILLNGSPIASLNEATLRQTISVVPQRVHLFSATLRDNLLLASPGSSDEALAEIL
                     RRVGLEKLLEDAGLNSWLGEGGRQLSGGELRRLAIARALLHNAPLVLLDEPTEGLDAT
                     TESQILELLAEMMHEKTVLMVTHRLRGLSRFQQIIVMDNGQIIEQGTHAELLARQGRY
                     YQFKQGL"
     gene            complement(7508..9274)
                     /gene="cydD"
                     /locus_tag="ACHKBL_002444"
     CDS             complement(7508..9274)
                     /gene="cydD"
                     /locus_tag="ACHKBL_002444"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0042883 - cysteine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HFO5819302.1"
                     /translation="MNKSRQKELTRWLKQQSVISQRWLNISRLLGFVSGILIIAQAWF
                     MARILQHMIMENIPREALLLPFTLLVLTFVLRAWVVWLRERVGYHAGQHIRFAIRRQV
                     LDRLQQAGPAWIQGKPAGSWATLVLEQIDDMHDYYARYLPQMALAVSVPLLIVVAIFP
                     SNWAAALILLGTAPLIPLFMALVGMGAADANRRNFLALARLSGHFLDRLRGMETLRIF
                     GRGEAEIESIRSASEDFRQRTMEVLRLAFLSSGILEFFTSLSIALVAVYFGFSYLGEL
                     DFGHYDTGVTLAAGFLALILAPEFFQPLRDLGTFYHAKAQAVGAADSLKTFMETPLAH
                     PQRGEAELASTDPVTIEAEDLFITSPEGKTLAGPLNFTLPAGQRAVLVGRSGSGKSSL
                     LNALSGFLSYQGSLRINGIELRDLSPESWRKHLSWVGQNPQLPAATLRDNVLLARPDA
                     SEHELQTALDNAWVSEFLPLLPQGVDTPVGDQAARLSVGQAQRVAVARALLNPCSLLL
                     LDEPAASLDAHSEQRVMEALNAASLRQTTLMVTHQLEDLADWDVIWVMQDGQIIEQGR
                     YAELSVAGGPFATLLAHRQEEI"
     gene            complement(9397..10362)
                     /gene="trxB"
                     /locus_tag="ACHKBL_002445"
     CDS             complement(9397..10362)
                     /gene="trxB"
                     /locus_tag="ACHKBL_002445"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HFO5819303.1"
                     /translation="MGTTKHSKLLILGSGPAGYTAAVYAARANLQPVLITGMEKGGQL
                     TTTTEVENWPGDPNDLTGPLLMERMHEHATKFETEIIFDHINKVDLQNRPFRLTGDSG
                     EYTCDALIIATGASARYLGLPSEEAFKGRGVSACATCDGFFYRNQKVAVIGGGNTAVE
                     EALYLSNIASEVHLIHRRDGFRAEKILIKRLMDKVENGNIILHTNRTLEEVTGDQMGV
                     TGVRLRDTQNSDNIESLDVAGLFVAIGHSPNTAIFEGQLELENGYIKVQSGIHGNATQ
                     TSIPGVFAAGDVMDHIYRQAITSAGTGCMAALDAERYLDGLADAK"
     gene            10906..11400
                     /gene="lrp"
                     /locus_tag="ACHKBL_002446"
     CDS             10906..11400
                     /gene="lrp"
                     /locus_tag="ACHKBL_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HFO5819304.1"
                     /translation="MVDSKKRPGKDLDRIDRNILNELQKDGRISNVELSKRVGLSPTP
                     CLERVRRLERQGFIQGYTALLNPHYLDASLLVFVEITLNRGAPDVFEQFNTAVQKLEE
                     IQECHLVSGDFDYLLKTRVPDMSAYRKLLGETLLRLPGVNDTRTYVVMEEVKQSNRLV
                     IKTR"
     gene            11535..15602
                     /gene="ftsK"
                     /locus_tag="ACHKBL_002447"
     CDS             11535..15602
                     /gene="ftsK"
                     /locus_tag="ACHKBL_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFO5819305.1"
                     /translation="MSQEYTEDKEVTLTKLSSGRRLLEALLILIVLFAVWLMAALLSF
                     NPSDPSWSQTAWHEPIHNLGGMPGAWLADTLFFIFGVMAYTIPVIIVGGCWFAWRHQS
                     SDEYIDYFAVSLRIIGVLALILTSCGLAAINADDIWYFASGGVIGSLLSTTLQPLLHS
                     SGGTIALLCVWAAGLTLFTGWSWVTIAEKLGGWILNILTFASNRTRRDDTWVDEDEYE
                     DDEEYEDENHGKQHESRRARILRGALARRKRLAEKFINPMGRQTDAALFSGKRMDDEE
                     EITYTARGVAADPDDVLFSGNRATQPEYDEYDPLLNGAPITEPVAVAAAATTATQSWA
                     APVEPVTQTPPVASVDVPPSQPTVAWQPVPGPQTGEPVIAPAPEGYPQQPQYAQPAVQ
                     YNEPLQQPVQPQQPYYAPAAEQPVQQPYYAPAAEQPVQQPYYATAAEQSAQQPYYAPA
                     PEQTAAGNAWQAEEQQSTFAPQSTYQTEQTYQQPAAQEPLYQQPQPVEQHPVVEPEPV
                     VEETKPARPPLYYFEEVEEKRAREREQLAAWYQPIPEPVKEPEPIKSSLKAPSVAAVP
                     PVEAAAAVSPLASGVKKATLATGAAATVAAPVFSLANSAGPRPQVKEGIGPQLPRPKR
                     IRVPTRRELASYGIKLPSQRAAEEKAREAQRNQYDSGDQYNDDEIDAMQQDELARQFA
                     QTQQQRYGEQYQHDVPVNAEDADAAAEAELARQFAQTQQQRYSGEQPAGANPFTLDDF
                     EFSPMKALLDDGPHEPLFTPIVEPVQQPQQPIAPQQQYQQPQQPVAPQPQYQQPQQPV
                     APQQQYQQPQQPVAPQPQYQQPQQPVAPQPQYQQPQQPVAPQPHDTLLHPLLMRNGDS
                     RPLHKPTTPLPSLDLLTPPPSEVEPVDTFALEQMARLVEARLADFRIKADVVNYSPGP
                     VITRFELNLAPGVKAARISNLSRDLARSLSTVAVRVVEVIPGKPYVGLELPNKKRQTV
                     YLREVLDNAKFRDNPSPLTVVLGKDIAGEPVVADLAKMPHLLVAGTTGSGKSVGVNAM
                     ILSMLYKAQPEDVRFIMIDPKMLELSVYEGIPHLLTEVVTDMKDAANALRWCVNEMER
                     RYKLMSALGVRNLAGYNEKIAEADCMMRPIPDPYWKPGDSMDAHHPVLKKEPYIVVLV
                     DEFADLMMTVGKKVEELIARLAQKARAAGIHLVLATQRPSVDVITGLIKANIPTRIAF
                     TVSSKIDSRTILDQAGAESLLGMGDMLYSGPNSTLPVRVHGAFVRDQEVHAVVQDWKA
                     RGRPQYVDGITSDSESEGGAGGFDGAEELDPLFDQAVQFVTEKRKASISGVQRQFRIG
                     YNRAARIIEQMEAQGIVSEQGHNGNREVLAPPPFD"
     gene            15761..16372
                     /gene="lolA"
                     /locus_tag="ACHKBL_002448"
     CDS             15761..16372
                     /gene="lolA"
                     /locus_tag="ACHKBL_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010354356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HFO5819306.1"
                     /translation="MKKIAITCALLSSLVASSVWADAASDLKSRLDKVSNFHASFTQK
                     VTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWL
                     KDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQ
                     FSAVEQDDQRSSYQLKSQQNGAVDAAKFTFTPPQGVTVDDQRK"
     gene            16383..17726
                     /gene="rarA"
                     /locus_tag="ACHKBL_002449"
                     /gene_synonym="mgsA"
     CDS             16383..17726
                     /gene="rarA"
                     /locus_tag="ACHKBL_002449"
                     /gene_synonym="mgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein
                     RarA"
                     /protein_id="HFO5819307.1"
                     /translation="MSNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAAGKPLPRA
                     IEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNR
                     NAGRRTILFVDEVHRFNKSQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVY
                     LLKSLSTEDIEQVLTQAMEDKTRGYGGQDIVLPDETRRAIAELVNGDARRALNTLEMM
                     ADMAEVDDSGKRVLKPELLTEIAGERSARFDNKGDRFYDLISALHKSVRGSAPDAALY
                     WYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIA
                     QAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPTKLMKEMGYGQEYRYA
                     HDEANAYAAGEVYFPPEIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPIKRYR"
     gene            17817..19109
                     /gene="serS"
                     /locus_tag="ACHKBL_002450"
     CDS             17817..19109
                     /gene="serS"
                     /locus_tag="ACHKBL_002450"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HFO5819308.1"
                     /translation="MLDPNLLRNEPDAVAEKLARRGFKLDVDKLGALEERRKVLQVKT
                     ENLQAERNSRSKSIGQAKARGEDIEPLRLEVNKLGEELDAAKAELDALQAEIRDIALT
                     IPNLPADEVPVGKDENDNVEVSRWGTPREFDFEVRDHVTLGEMHSGLDFAAAVKLTGS
                     RFVVMKGQIARMHRALSQFMLDLHTEQHGYSENYVPYLVNQDTLYGTGQLPKFAGDLF
                     HTRPLEEEADTSNYALIPTAEVPLTNLVRGEIIDEDDLPIKMTAHTPCFRSEAGSYGR
                     DTRGLIRMHQFDKVEMVQIVRPEDSMAALEEMTGHAEKVLQLLGLPYRKIILCTGDMG
                     FGACKTYDLEVWIPAQNTYREISSCSNVWDFQARRMQARCRSKSDKKTRLVHTLNGSG
                     LAVGRTLVAVMENYQQADGRIEVPEVLRPYMNGLEYIG"
     gene            complement(19350..19490)
                     /locus_tag="ACHKBL_002451"
     CDS             complement(19350..19490)
                     /locus_tag="ACHKBL_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFO5819309.1"
                     /translation="MPQKTIIVSILCLTMLLTVWMIHRSPCEFRLNIMWSEFAAFLQC
                     KP"
BASE COUNT         5016 a         4904 c         5242 g         4568 t
ORIGIN      
        1 gttggaactt ttaccccatt gggggaaaag gtgcatacct gaatcatcat tatggtgatc
       61 aagggtatat aaagcccacc agcctgatta aaggttaacc ggaaaaagcc aggtatggag
      121 ggagtggtta gccacgaaca agccggacac caccagcaga aggtattatg attttcgtaa
      181 tacctttagc aaagcgtgaa tcatcattac cgtagggatg gtcataatga ccacaccttc
      241 ccccaccgga acaaggaagg ctggcctacg aaaatttcgt aaaattcccc ccaaaatccc
      301 cccacggttg cccgttaagt gcaagcgcac aggaacgacc tcgctaatca gttaccagcg
      361 ctcacgctca aatatccaca gaaacgggga taaatcgcac tctggcgcaa tgttcactat
      421 cggggcaagc attcaccgga aaacgtcata caggcttaca ggcggcttaa aattcgatgg
      481 gcggataaca gggcgggatc gggaggctta cgaaaatttc gtaaccccct acacaaaaag
      541 tgcaggttac tacccgaaaa gtgtagtgcc attgctgggg caaaaaaaac cgcagtgtga
      601 taacactacg gcacgccacc aaagaaacat aacaaggaat cagcaatgag taatacctgt
      661 aagccttcgg ggtgattgta acacatgagc aatcttttac tagaagctgt ttttcactgg
      721 atcacatatc gttatgggga ttcccatatc ggagagcatc caccagcttc ataacggatg
      781 gccggaaaat gataacggtt aaagcgtgtt gatctttgtt cgtggttgtt cgatattgtt
      841 cgtgaaacgg tgtagttaat ggtgtagtta atttcgctgt ttttgacagg gttgttattt
      901 gcaacacaat gaaaaataaa gactaataaa tgaagtgtta taaatgatag ttatctatca
      961 tgtggagtag attggtcagg caaataagct cttgtcagcg gcagggcgtt ctgccgataa
     1021 ccgtaaccga agatgataac tgacaatggg taaaacgaac gactggctgg actttgatca
     1081 actggcggaa gaaaaagttc gcgacgcgct aaaaccgcca tctatgtata aagtgatatt
     1141 agtcaatgat gattatactc cgatggagtt tgttattgac gtgttacaaa aattcttttc
     1201 ttatgatgta gaacgtgcaa cgcaattgat gctcgctgtt cactaccagg ggaaggccat
     1261 ttgtggagtc tttaccgctg aggttgcaga aaccaaagtg gcgatggtga acaagtacgc
     1321 gagggagaat gagcatccat tgctgtgtac gctagaaaaa gcctgaatgc aggcataaaa
     1381 attgggggag gtgcctatgc tcaatcaaga actggaactc agtttaaata tggctttcgc
     1441 cagagcgcgc gagcaccgtc atgagtttat gaccgtcgag cacttgttac tggcgctgct
     1501 cagtaaccca tctgcccggg aggcgctgga agcgtgctct gtggatttgg ttgcgctccg
     1561 tcaggaactg gaagccttta ttgaacaaac cacacccgtt ctgcctgcca gtgaagagga
     1621 gcgcgacaca cagccgacgc tgagttttca gcgtgtactg caacgtgcgg tcttccatgt
     1681 ccagtcctcc ggtcgcaatg aggttaccgg tgcaaacgtt ctggtcgcta tctttagcga
     1741 acaggagtcg caggcggcat atctgttgcg caaacatgaa gtcagccgtc tcgatgtggt
     1801 gaactttatc tctcatggca cgcgtaaaga cgagccgaca cagtcttctg atcctggcag
     1861 ccagccaaac agcgaagaac aagctggtgg ggaggaacgt atggagaatt tcacgacgaa
     1921 cctgaatcag cttgcgcgag tgggcggaat cgacccactg attggtcgtg agaaggagct
     1981 ggagcgtgct attcaggttc tctgccgtcg ccgtaaaaac aacccgctgc tggtggggga
     2041 atctggtgtc ggtaaaaccg cgattgcgga ggggcttgca tggcgaattg ttcagggcga
     2101 tgtgccggaa gtgatggctg actgtacgat ttactctctc gatatcggtt ctctgttagc
     2161 gggcacaaaa tatcgcggcg actttgaaaa acgttttaaa gcgttgctca agcagctgga
     2221 gcaagacact aacagcatcc tgtttattga tgagatccac accattatcg gtgcgggtgc
     2281 agcgtctggt ggccaggtcg atgcagctaa cctgatcaaa ccgttgctct ccagcggtaa
     2341 aattcgcgta attggttcga caacctatca ggagttcagc aacattttcg agaaagaccg
     2401 tgctctggcg cgtcgcttcc agaaaattga tattactgaa ccgtcaatcg aagaaactgt
     2461 tcaaatcatc aatggcctga aaccgaagta tgaagcgcac cacgacgtgc gttataccgc
     2521 aaaagcggtg cgtgcagcgg tagagctggc ggtgaaatac attaacgatc gtcatctgcc
     2581 ggataaagcc attgacgtta tcgacgaagc gggcgctcgc gcacgcctga tgccggtaag
     2641 caaacgcaag aaaaccgtta atgtggcgga tattgagtcc gtggtggccc gtattgcgcg
     2701 cattccagag aagagtgttt cgcagagtga ccgcgatacc ctgaaaaacc tcggcgatcg
     2761 cctgaaaatg ctggtcttcg gtcaggataa agccattgag gcgctgactg aagccattaa
     2821 gatggcgcgt gcaggtttag gtcacgaaca taaaccggtc ggttcgttcc tgtttgccgg
     2881 tcctaccggg gtcgggaaaa cagaggtgac ggtacagctt tcgaaagcgt tgggcattga
     2941 gcttctgcgc tttgatatgt ccgagtatat ggaacgccat accgtcagcc gtctgattgg
     3001 tgcgcctccg ggatacgttg gttttgatca gggcggtttg ctgactgatg cggtcatcaa
     3061 gcatccacat gcggtgctgc tgctggacga aatcgagaaa gcgcacccgg acgtgttcaa
     3121 tattctgttg caggtgatgg acaacggtac gctgaccgat aacaacggac gcaaagcgga
     3181 cttccgtaac gtggtattgg tgatgaccac caacgctggg gtacgagaaa ctgagcgtaa
     3241 atcgattggt cttatccacc aggacaacag taccgatgcg atggaagaga tcaagaagat
     3301 ctttacgccg gagtttcgta accgtctcga caacattatc tggttcgatc atctctccac
     3361 cgacgtgatc catcaggtag tggataaatt catcgtcgag ttgcaggttc agctggatca
     3421 gaaaggtgtt tctctggaag tgagccagga agcgcgtaac tggctggccg agaaaggtta
     3481 cgaccgggca atgggcgcac gtccgatggc gcgtgtcatc caggacaacc tgaaaaaacc
     3541 gctcgccaac gaactgttgt ttggttcgct ggtggacggc ggtcaggtga cggttgcgct
     3601 ggataaagag aaaaatgagc tgacttatgg attccagagt gcacaaaagc acaaggcgga
     3661 agcagcgcat taatctaatt gttaggtagg ttggtgaagt ccgtaatctc gaaagaggtt
     3721 acggactttt tgtttatggg gtggaggagg ttctgaccct ttttttgatg atgatggtaa
     3781 tttgttgata attagtgctg cgggaaggta aggataaaaa agggtggtag caggaaaatg
     3841 agatggtttt gctttattaa caacgggcta gacgtgtagt atttgagttc actgccgtac
     3901 aggcagctta gaaagccaac cgaaagaaaa actcgatttt tataccgttc actgccgtac
     3961 agacagataa aatgcgaaaa aaaaaacgta ctttcgtacg agctcttctt tgaatatggc
     4021 ggtgaggggg ggattgactc gcttcgctcg ccctgcgggc agcccactca ctgtgttcgt
     4081 ggtctgtcca actggctgcg ccagttgtcg aaccccggtc ggggcttctc atcccccctg
     4141 gagtgcaata tgcgaaaaaa aagctcgcac tttcgtacga gctcttcttt gaatatggcg
     4201 gtgagggggg gattcgaacc cccgatacgt tgccgtatac acactttcca ggcgtgctcc
     4261 ttcagccact cggacacctc accaaattgt tttgctacca aacctcatgg gtggcaacgg
     4321 ggcgctacta tagggagttg gagtaaaacg gtcaagaaga attttaatga taattattgt
     4381 ttgctcatac tgtaaacaag ttgtgcagta tatctacatc gagacaagtt atggacttat
     4441 acttccaaag tacttcatac atatcacaaa ataaaaaggc cggctaaacc gaccttttac
     4501 tcgttctttc tcttcgccca tcaggcggta aaacaatcag cgactacgga agacaatgcg
     4561 gcctttgctc aggtcgtacg gggtcagttc aacagtcact ttgtcgcccg tcaggatgcg
     4621 gatgtagttt ttgcgcattt taccggagat gtgtgcagta accacgtgac cgttttctaa
     4681 ctctacgcgg aacatggtat taggcaacgt ttcaagaacg gtaccttgca tttcaatatt
     4741 gtcttctttg gccatctaat cctctggggt atcactaccg taatttgaac cggcaagata
     4801 atgccgaagt tctgtaaata agtaaagatt tgcgcgctaa atcgcaacaa acaggttcgg
     4861 cacattactc cgaaaacaca cggctaagcc gcaccaaaag cgcaacgtat aagggagcgg
     4921 tgagataaac gatgggcgtt acctgacgcg aaaaattcct tatcggcagc ggggtaatga
     4981 gcgtaaccaa ctctgcgacc gcaattataa cactctgggg agaaatgtgc cgaaaacatt
     5041 cattcttgtg gtgaaaacaa gcaccgtggt acccagaaat tattcggcaa tcgtccgagg
     5101 cgcatttgat tgagataatt gaggtaatcc cggcggggaa tttcgcaggc accaagcgac
     5161 gctgtgtgat cgttaaggac ctggcagtcg ataagtttac cgccatgacc aataaattcc
     5221 tcacagaata ccagaagcgc cgttttagac gcattttcca tccggctgaa catggactcg
     5281 ccacaaaata gcgttccctg ggccacgccg tacataccgc cgacaagctc atcttcacgc
     5341 cagacttcaa tagagtgggc atgaccgagt tcgtgaaggc gatggtaggc ttcgaccacg
     5401 ccacgcgtga tccaggttcc ttcttcgcga tcgctggcac agccttcaat gacctgacca
     5461 aaagcgtaat tcatcgtgac tcgatagggc gagcgtttat gaaatcgctt catactacgg
     5521 ctgatatgca gtgattcagg ccaaagcacc gcgcggggat cgggcgacca ccagaggatg
     5581 gggtcacctg gagaaaacca cggaaaaata ccacgctggt aagccattaa caggcgcgca
     5641 gggctaagat cgcctcccag tgccagcagg ccgttaggct cacgtaatgc gccttccggg
     5701 gaagggaagg ctattgaatg gcgagaaagc tgaaccaggc gcatgaccgc aaaactccac
     5761 gcaagtcgga tcgttcaata atagcttaca aaccctgctt aaactggtaa taacgcccct
     5821 gtctggcaag cagttctgcg tgagtacctt gctcaataat ttgcccgttg tccatcacta
     5881 ttatttgttg gaaacgagag agtccgcgaa gtcgatgggt gaccattaac accgttttct
     5941 catgcatcat ttctgcaagc aattcaagga tctgactttc ggttgtggca tctaagcctt
     6001 ctgtaggttc atccagcaat accagcggcg cattatgtaa cagcgcacgg gcgatagcca
     6061 gacggcgtag ttcaccaccg gagagctggc gtccgccttc acccaaccaa ctgttgagac
     6121 ctgcatcctc gagcagcttc tccagaccaa cgcgacgtaa gatctccgcc agagcctcat
     6181 cactactgcc aggcgaggcg agtaacagat tatcacgcag cgtggcgcta aacagatgca
     6241 ctcgctgagg aacaacgctg atggtctgtc gtagagtcgc ttcattcagg ctggctatgg
     6301 ggctaccgtt aagcaaaatc tcgccctgtt gcgggtccca tgcgcgggtc agcagttgta
     6361 acagtgttga tttgccgcat ccggttcgcc cgagaatcgc tatatgttcc ccggcgttta
     6421 cctgaagaga aatcccttta agtgcctgtt gggattgctc cggataattg aattgaacat
     6481 cccgtaacgt cagcgaaacg cgatcggcaa cacgagtttg ggtatcaggg aaggtgactt
     6541 ccggtttttg atccgttaag tcagtgatcc gtacggcaga ggcaatgact tgccccagat
     6601 gctgaaatgc acccgttact ggtgccagtg cttcaagcgc ggctaacgcg cagaagacaa
     6661 acagggcaat taacgcgccg ggttgagcat tgccgccaac gccgccagac gccatccaca
     6721 gcatcaggat caccgctaac gcgccaatga gcagcattat cgcttgcgac aatgcggtga
     6781 gttcagattg acgacgttgc gcttccagcc actgaatttc tgtattctct aattgcgtgc
     6841 gataacgatc gctggcacca aagatggtca gctcagcttg cccttgcagc caggccgtca
     6901 gttgttggcg atactgcccg cgaagatgag tcagattttg cccggtgctt tttcccgcac
     6961 gataaaacag tggtggcata aggaaaagcg tcagtaacat aatgccgccc agcgtaaagg
     7021 cgagggtgaa atcaaggaaa cttaacccga ttgtcaccac cataatcacc acaaaagcgc
     7081 ccaccagcgg cgagataacg cgcaggtaaa gatgatcgag cgtatcaaca tccgccacca
     7141 cgcggttgag caattcgccc tgacgatagc gcgccagtcc ggcaggggat aggggcagca
     7201 atttgctgaa ggtgtaaatg cgcagatgct gcaacacgcg gaaagtcgcg tcgtgactta
     7261 ccagacgttc aaaatagcgc ccggcagtac gggtgattgc tgcgccacgc acgcccgcag
     7321 cgggtagcat atagttgaag ctgtacagtc cggcaacccc cgctaccgct gaggcggaga
     7381 ggaaccagcc ggaaagtgtc aacagaccga tactggcgag cagcgtgaca attgccagca
     7441 caataccaag acttaacatc catttatgac gtttatacag tgccagatag ggtagcaaag
     7501 cgcgcattta aatctcctcc tgacgatggg ccagcaatgt ggcgaaagga ccaccagcca
     7561 cgcttaattc cgcgtaacgt ccttgctcaa taatctgacc atcctgcatt acccaaatga
     7621 catcccagtc agcaagatct tctaactggt gggtgaccat taacgttgtc tggcgcagag
     7681 aggcggcatt aagcgcctcc attacgcgct gttcactgtg agcatcaagg ctggcagcgg
     7741 gttcatccaa cagtaatagc gaacagggat ttagtaatgc tcgggccacc gccacgcgct
     7801 gcgcctgccc cacggaaagg cgggcagcct ggtcgccaac aggcgtatca acgccttgtg
     7861 gcaggagcgg tagaaactcg ctgacccagg cgttatccag cgctgtttgt aactcgtgtt
     7921 cgctggcatc aggtcgtgcc agtagtacgt tatcccgtag tgttgctgcc ggtaattgtg
     7981 ggttttgccc aacccaggag agatgcttac gccatgattc cggtgataaa tcgcgtaatt
     8041 ctatcccgtt gattcgtagc gatccctgat atgagagaaa accagaaagc gcgttcagca
     8101 gtgagctttt acctgaaccg ctgcgaccaa ccaacactgc acgttggcct gctggcaaag
     8161 taaagttcag cggtccagcc agcgttttac cttctggtga tgtaataaac agatcctcgg
     8221 cttcaatggt caccggatcg gttgatgcta attccgcctc accacgttgc ggatgggcta
     8281 gcggggtttc cataaacgtt ttcagactgt cagctgcgcc aacagcctgg gctttagcat
     8341 gataaaacgt accgagatcg cgtaatggct ggaaaaactc cggcgcaagg atcagggcca
     8401 gaaaacccgc agccagcgtc acgccagtat catagtgacc aaaatccagc tcgccgagat
     8461 aggaaaaacc aaagtagacc gccaccagag caattgacag cgaggtaaaa aattcgagaa
     8521 tgccggagga taaaaacgcc agccgtagca cttccattgt ccgttggcgg aaatcttccg
     8581 aagcagaacg aatactttca atttcagctt caccacgacc aaaaatacgc aatgtttcca
     8641 tgccgcgcag gcgatcgagg aaatgcccac ttaagcgagc aagagcgaga aagttacgtc
     8701 ggttagcatc ggcagccccc attccaacca gcgccataaa caacggaatt aacggtgcag
     8761 tgcccagcag aataagcgcc gcagcccagt tagacgggaa gatcgccacc acaatcagca
     8821 aaggcacgga cactgccagc gccatttgcg gcaggtagcg tgcatagtaa tcatgcatat
     8881 cgtcaatttg ctcgagtacc agcgtcgccc agctccccgc cggtttaccc tgaatccacg
     8941 ctggccctgc ttgttgcaga cggtcgagaa cctgacggcg gatggcaaag cggatatgct
     9001 gcccggcgtg ataacccacc cgttcgcgta accagaccac ccatgcgcgc agtacaaagg
     9061 tcagaaccag taacgtaaag ggaagcagca gggcttcacg gggaatattc tccataatca
     9121 tatgttgcag aattcgcgcc atgaaccagg cctgggcaat gatcaatatg ccgctcacaa
     9181 agcccagcag acgagaaata ttcagccaac gttgggagat gacgctttgc tgttttaacc
     9241 agcgggttaa ctctttttga cgggatttat tcattgcacg cttagcaggt gagttatcag
     9301 aattatttgc agagcaatgt tacaacgagg aaaaaataaa ggcgacccat agtcgcatgg
     9361 tgtcgccttc tttacttttg ttactgattt gtaaaattat tttgcgtcag ctaaaccatc
     9421 gaggtagcgt tccgcatcaa gtgctgccat gcagcctgta ccagcagatg taatagcctg
     9481 gcgataaatg tgatccatca cgtcgcctgc ggcaaagacg ccagggatgc tggtctgggt
     9541 ggcattacca tgaatacccg actgtacttt gatgtagccg ttttccagtt ccagctgccc
     9601 ttcgaaaata gccgtattcg ggctgtgacc gatagcaaca aacagaccgg caacgtcgag
     9661 tgactcgatg ttatcgctgt tttgcgtatc gcgcagacga acgccagtga cgcccatctg
     9721 atcgccggtc acctcttcca gcgtacggtt ggtgtgtaga atgatgttgc cgttctccac
     9781 tttatccatc agacgcttaa taaggatttt ttccgcgcgg aaaccgtcac ggcggtgaat
     9841 cagatgcact tccgaagcga tgttagacag atacagcgcc tcttcaaccg cggtattgcc
     9901 gccgccgatg accgcaactt tctggttgcg atagaagaaa ccgtcgcagg tagcacaagc
     9961 agaaacccca cggcctttaa aggcttcttc agatggcagg cctagatagc gtgcagaagc
    10021 tccggtggca ataatcagcg cgtcgcaagt gtattcgccg ctatcgccag tcagacggaa
    10081 cggacgattc tgcaaatcaa ctttgttgat gtgatcaaag atgatttcag tttcaaattt
    10141 ggtggcatgt tcgtgcatgc gctccattaa taacggaccg gtcagatcgt ttggatcgcc
    10201 aggccagttt tccacttccg tggtggtggt cagttggccg cctttttcca tgccggtaat
    10261 cagcacaggt tgcaggttgg cgcgcgccgc gtagacagca gcggtatatc ccgccgggcc
    10321 tgaacccagg ataagcagtt tactgtgttt ggtcgtgccc atgagatccc catagttgtt
    10381 ggcagacaat gggcaggatt gtagggaatt tacagacgta aaaaaagagt atgacgattt
    10441 tgttaacaat ttgtgcaatc ggcagcatcg ataagcaggt caaattctcc cgtcattatc
    10501 acctctgcta cttaaatttc ccgctttata agccgattaa atgatgaata aacgcccctg
    10561 ttaatgaata tctggcatgt tgtactaaaa atcgatgttt tgctttgaca atcccctggt
    10621 gttttgcgaa aacattcgag gaagaaaaaa acagtattct tatatgcgca taaccatgca
    10681 tgtaaatacc atgtttaccg tgctagtgaa atctacgtat ggcgtggaca gacgccattc
    10741 gtgatgtcga tagctgccac aaggcaacgg tcttctcacc gtagacccag gcattgcgcg
    10801 ccgtgaatct tcatgatttc ggtctatcgt gacgggtagc gactctgaac agtgatgttt
    10861 cagggtcaga caggagtagg gaaggaatac agagagacaa taataatggt agatagcaag
    10921 aagcgccctg gcaaagatct cgaccgtatc gatcgtaaca ttcttaatga gttgcaaaag
    10981 gatgggcgta tttctaacgt cgagctttct aaacgtgtgg gactttcccc aacgccgtgc
    11041 cttgagcgtg tgcgtcggct ggaaagacaa gggtttattc agggctatac ggcgctgctg
    11101 aacccccatt atctggatgc atcacttctg gtattcgttg agattactct gaatcgtggc
    11161 gcaccggatg tgtttgaaca attcaatacc gctgtacaaa aacttgaaga aattcaggag
    11221 tgtcatttag tatccggtga tttcgactac ctgttgaaaa cacgcgtgcc ggatatgtca
    11281 gcttaccgta agttactggg ggaaaccctg ctgcgtctgc ctggcgtcaa tgacacacgg
    11341 acatacgttg ttatggaaga agtcaagcag agtaatcgtc tggttattaa gacgcgctaa
    11401 cacggaacag gtgcaaaatc ggcgtatttt gattacactc ctgttaatcc atacagcaac
    11461 agtactgggg taacctggta ctgttgtccg ttttagcatc gggcaggaaa agcctgtaac
    11521 ctggagagcc tttcttgagc caggaataca ctgaagacaa agaagtcaca ttgacaaagt
    11581 taagtagcgg ccgccgcctt ctggaggcgt tgctgatcct tattgtcctg tttgccgtct
    11641 ggttgatggc tgccttacta agctttaacc cttcggaccc cagctggtcg caaacggcct
    11701 ggcatgaacc tatccataat ttaggtggga tgcccggtgc gtggctggca gatacgctgt
    11761 tctttatttt tggcgtgatg gcttacacca ttcccgtcat tattgtcggc ggttgttggt
    11821 ttgcctggcg tcatcagtcc agcgacgaat acattgatta ttttgccgtt tcgctacgca
    11881 tcattggcgt tttggcgctc atccttacct cctgtggtct ggcggcaatc aacgctgacg
    11941 atatctggta ttttgcctcc ggtggcgtca ttggcagctt actaagcact acgctacaac
    12001 cactgctaca cagtagcggg ggaactattg cgctgctctg cgtttgggct gcgggtctga
    12061 cgctgttcac cggttggtca tgggtgacca ttgctgaaaa actcggcggc tggattttaa
    12121 acattctcac cttcgccagt aatcgtaccc gtcgcgatga tacctgggtc gatgaagatg
    12181 agtatgaaga cgacgaagaa tatgaagatg aaaatcacgg taaacagcat gaatcacgcc
    12241 gtgcccgtat tcttcgcggc gcgttagcgc gtcgtaaacg gttggcggaa aaattcatta
    12301 atccgatggg gcggcaaaca gatgctgcgt tgttctccgg taagcggatg gatgatgaag
    12361 aagagattac ctacactgca cgcggtgtgg ctgccgaccc tgacgacgtc ctcttttcgg
    12421 gcaatcgtgc aacgcagcca gaatatgacg aatacgatcc attattaaac ggtgcgccaa
    12481 ttaccgaacc tgttgctgta gcagctgctg ctaccacggc gacacaaagc tgggctgcgc
    12541 cggttgaacc tgtgactcag acgccgcctg ttgcctctgt tgatgttcca ccttcgcaac
    12601 ctacagtagc ctggcagcct gtaccgggtc cacaaacggg agagccggtg attgctccgg
    12661 caccggaagg ttacccacag cagccacaat atgcgcagcc tgcagtgcaa tataatgagc
    12721 cgctgcaaca acctgtacag ccacagcagc cgtattatgc gccagcagcg gaacaacctg
    12781 tgcaacaacc ttactacgct cctgcggcgg aacaacctgt tcagcagccg tattatgcaa
    12841 ctgcggcgga acagtctgcg caacagccgt attacgcccc tgcgccagaa caaacggcgg
    12901 caggtaacgc ctggcaagcc gaagagcagc aatccacttt tgctccacag tctacatacc
    12961 agactgagca aacttatcag cagccagccg ctcaggagcc gttgtaccaa cagccgcaac
    13021 ccgttgaaca gcaccctgtt gtggagcctg agcccgttgt agaagagaca aaacccgcgc
    13081 gtccgccgct ttactatttt gaagaagtgg aagagaagcg tgcccgtgaa cgtgaacaac
    13141 ttgcggcctg gtatcaaccg attcctgaac cggttaaaga accagaaccg atcaaatctt
    13201 cgctgaaagc accttctgtt gcagcagtac ctccagtgga agccgctgcc gctgtttctc
    13261 cgctggcatc tggcgtgaaa aaagcgacac tggcgacggg ggctgccgca accgttgccg
    13321 cgccagtctt cagtctggca aatagcgctg gaccgcgtcc gcaggtcaaa gaggggattg
    13381 gcccgcagtt gccacgaccg aaacgtatcc gcgtgccaac tcgtcgtgaa ctggcgtctt
    13441 acggtattaa gctgccctca cagcgtgcgg cagaagaaaa agcccgtgaa gctcagcgca
    13501 atcagtacga ttccggcgat cagtataacg atgatgaaat cgatgcgatg cagcaggatg
    13561 aactggcacg tcagttcgcc cagacacagc agcaacgcta tggcgaacag tatcaacatg
    13621 atgtgcccgt gaacgcagaa gatgcagatg ctgcggcaga ggctgaactg gctcgtcagt
    13681 ttgctcaaac tcaacaacaa cgttattccg gcgaacaacc tgctggggcg aatccgttca
    13741 ctctggatga ttttgaattt tcgccaatga aagcgttgct ggatgatggt ccacacgaac
    13801 cgctgtttac gccaattgtt gaacctgtac agcagccgca acaaccgatt gcaccgcagc
    13861 aacagtatca gcagccgcag caaccagttg cgccgcagcc gcagtatcag cagccgcaac
    13921 agccggttgc accgcagcag caatatcagc agccacaaca accggttgca ccgcagccgc
    13981 aatatcagca gccgcaacag ccggttgcac cacagccgca atatcagcag ccgcaacagc
    14041 cagttgcgcc gcagccgcat gataccctgc ttcatccgct gttgatgcgc aatggtgaca
    14101 gccgtccgtt gcataaaccg acgacgccgc tgccttctct ggatttgctg acgccgccgc
    14161 cgagcgaagt ggagccggta gatacctttg cgcttgaaca aatggcgcgc ctggtggaag
    14221 cgcgtctggc tgatttccgt attaaagccg atgtcgtcaa ttattctccg gggccggtta
    14281 tcactcgctt tgaattgaac ctggcaccgg gagtaaaagc ggcgcgtatt tctaacttgt
    14341 cacgtgacct tgcccgttca ctttcgacgg tggcggtgcg tgtcgttgaa gttattcctg
    14401 gcaaacccta tgtagggctg gagttaccga ataaaaaacg acaaaccgtt tatctgcgcg
    14461 aagttttgga taacgccaaa ttccgcgata atccgtcgcc attaaccgtg gtgctgggta
    14521 aagatatcgc cggtgagccg gtggttgccg atctggcgaa aatgccgcac ttgttggttg
    14581 cggggactac cggttccggt aagtctgtcg gtgtgaacgc gatgatcctg agcatgcttt
    14641 ataaagcaca gccagaagat gtgcgtttca tcatgatcga cccgaaaatg ctggagcttt
    14701 cggtttatga aggcatcccg catctgttaa cggaagtcgt tactgatatg aaagatgccg
    14761 ccaacgcgct gcgctggtgt gttaacgaga tggagcgtcg ttataaactg atgtctgcgc
    14821 tgggtgtgcg taatctggcg ggttataacg aaaaaattgc tgaagccgat tgcatgatgc
    14881 gtccgattcc agacccgtac tggaagccgg gtgatagtat ggatgcgcat catccggtgc
    14941 tgaaaaaaga accgtacata gtggtgttgg tggacgaatt tgccgacctg atgatgacgg
    15001 taggtaaaaa agtggaagag ctgatagcac gtctggcgca aaaagcccgt gccgcgggta
    15061 tccacctcgt actggcaacg cagcgtccat cggttgatgt tattactggt ctgattaaag
    15121 cgaatattcc gacccgtatc gcctttaccg tatccagtaa gattgactca cgtaccattc
    15181 ttgatcaggc tggcgcggaa tcactgctgg gtatggggga tatgctctac tctgggccga
    15241 actccacgtt gccggtacgt gtccatggtg cttttgtacg cgatcaggaa gttcatgccg
    15301 tggtgcagga ctggaaagcg cgtggtcgcc cacagtatgt tgacggcatc acctccgaca
    15361 gcgaaagcga aggtggtgcg ggtggtttcg atggcgctga agaactggat ccgttgttcg
    15421 atcaggcggt gcagtttgtc actgaaaaac gcaaagcgtc aatttcaggc gtacagcgtc
    15481 agttccgcat tggttataac cgtgcagcgc gtattatcga acagatggaa gcgcaaggga
    15541 ttgtcagcga acaggggcac aacggtaatc gtgaagtgct ggccccaccg ccgtttgact
    15601 aattaatgca tcgtaggccg gataaggcgc ggtagcgtcg catccggcac tctaacaact
    15661 gaaaattcag tattttcttc tttcctcaag ctgattatta gcctggaata gagagtagag
    15721 ggaactcccg atcgggagtg acgtaatttg aggaataatg atgaaaaaaa ttgccatcac
    15781 ctgtgcatta ctctcaagct tagtagcaag cagcgtttgg gctgatgccg caagcgatct
    15841 taaaagccgc ctggataaag tcagcaactt ccacgccagc ttcacacaaa aagtgactga
    15901 cggtagcggc gcggcggtgc aggaaggcca gggcgatctg tgggtgaaac gtccaaattt
    15961 attcaactgg catatgactc aacctgatga aagcattctg gtttctgacg gtaaaacact
    16021 gtggttctat aacccgttcg ttgagcaagc tacggcaacc tggctgaaag atgccaccgg
    16081 taatacgccg tttatgctga ttgcccgcaa ccagtccagc gactggcagc agtacaatat
    16141 caaacagaat ggcgatgact ttgtcctgac gccgaaagcc agcaatggca atctgaagca
    16201 gttcaccatt aacgtgggac gtgatggcac aatccatcag tttagcgcgg tggagcagga
    16261 cgatcagcgc agcagttatc agctgaaatc ccagcaaaat ggggctgtgg atgcagcgaa
    16321 atttaccttc accccgccgc aaggcgtcac ggtagatgat caacgtaagt agaggcacct
    16381 gagtgagcaa tctgtcgctc gatttttcgg ataatacttt tcaacctctg gccgcgcgta
    16441 tgcggccaga aaatttagca cagtatatcg gccagcaaca tttgttggct gcggggaagc
    16501 cgttgccgcg cgctatcgaa gccgggcatt tgcattctat gatcctctgg gggccaccgg
    16561 gtaccggcaa aacaaccctc gctgaagtga ttgcccgcta tgcgaacgct gatgtggaac
    16621 gtatttctgc tgtcacctct ggcgtgaaag agattcgcga ggcgattgag cgcgctcggc
    16681 aaaaccgcaa tgcaggtcgc cgcactattc tttttgttga cgaagttcac cgtttcaaca
    16741 aaagccagca ggatgcattt ctgccacata ttgaagacgg caccatcact tttattggcg
    16801 caaccactga aaacccgtcg tttgaactta actcggcact gctttcccgt gcccgtgtct
    16861 atctgctgaa atccctgagt acagaggata ttgagcaggt gcttactcag gcgatggaag
    16921 acaaaacccg tggctatggt ggtcaggata ttgttctgcc agatgaaaca cgacgtgcca
    16981 ttgctgaact ggtgaatggc gacgcgcgcc gggcgttaaa tacgctggaa atgatggcgg
    17041 atatggccga agtcgatgat agcggtaagc gggtcctgaa gcctgaatta ctgaccgaaa
    17101 tcgccggtga acgtagcgcc cgctttgata acaaaggaga tcgcttttac gatctgattt
    17161 ccgcactgca taagtcggta cgtggtagcg cacccgatgc ggcgctgtac tggtatgcgc
    17221 gaattattac cgctggtggc gatccgttat atgtcgcgcg tcgctgtctg gcgattgcgt
    17281 ctgaagacgt cggtaatgcc gatccacggg cgatgcaggt ggcaattgcg gcctgggatt
    17341 gctttactcg cgttggcccg gcggaaggtg aacgcgccat tgctcaggcg attgtttacc
    17401 tggcttgcgc gccaaaaagc aacgctgtct acactgcgtt taaagccgcg ctggcggatg
    17461 cacgcgaacg tccggattat gacgtgccgg ttcatttgcg taatgcgccg acgaaattaa
    17521 tgaaggaaat gggctacggg caggaatatc gttacgctca tgatgaagca aacgcttatg
    17581 ctgccggtga ggtttatttc ccgccggaaa tagcacaaac acgctattat ttcccgacaa
    17641 acaggggcct tgaaggcaag attggcgaaa agctcgcctg gctggctgaa caggatcaaa
    17701 atagccccat aaaacgctac cgttaatgtt atcgttgcgg taatgttgtt actgtatccc
    17761 tgtggtcgca ggctgtggcc acatctccca tttaattcga taagcacagg ataagcatgc
    17821 tcgatcccaa tctgctgcgt aatgagccag acgcagtcgc tgaaaaactg gcacgccggg
    17881 gctttaagct ggatgtagat aagctgggcg ctcttgaaga gcgtcgtaaa gtattgcagg
    17941 tcaaaacgga aaacctgcaa gcggagcgta actcccgatc gaaatccatt ggccaggcga
    18001 aagcgcgcgg ggaagatatc gagcctttac gtctggaagt gaacaaactg ggcgaagagc
    18061 tggatgcagc aaaagccgag ctggatgctt tacaggctga aattcgcgat atcgcgctaa
    18121 ccatccctaa cctgcctgca gatgaagtgc cggtaggtaa agacgaaaat gacaacgttg
    18181 aagtcagccg ctggggcacc ccgcgtgagt ttgactttga agttcgtgac catgtgacgc
    18241 tgggtgaaat gcactctggc ctcgactttg cagccgcagt taagttgacc ggttcccgct
    18301 ttgtggtaat gaaagggcag attgctcgca tgcaccgcgc actgtcgcag tttatgctgg
    18361 atctgcatac cgaacagcat ggctacagtg agaactatgt tccgtacctg gttaaccagg
    18421 acacgctgta cggtacgggt cagctgccga aatttgctgg cgatctgttc catactcgtc
    18481 cgctggaaga agaagcagac accagtaact atgcgctgat cccaacggca gaagttccgc
    18541 tgaccaacct ggtgcgcggt gaaatcatcg atgaagatga tctgccaatt aagatgaccg
    18601 cccacacccc atgcttccgt tctgaagccg gctcatatgg tcgtgacacc cgtggtctga
    18661 tccgtatgca ccagttcgac aaagttgaaa tggtgcagat cgtgcgcccg gaagactcaa
    18721 tggcggcgct ggaagagatg actggtcatg cagaaaaagt cctgcagttg ctgggcctgc
    18781 cgtaccgtaa aatcatcctt tgcactggcg acatgggctt tggcgcttgc aaaacttacg
    18841 acctggaagt atggatcccg gcacagaaca cctaccgtga gatctcttcc tgctccaacg
    18901 tttgggattt ccaggcacgt cgtatgcagg cacgttgccg cagcaagtcg gacaagaaaa
    18961 cccgtctggt tcataccctg aacggttctg gtctggctgt tggtcgtacg ctggttgcag
    19021 taatggaaaa ctatcagcag gctgatggtc gtattgaagt accagaagtt ctgcgtccgt
    19081 atatgaacgg actggaatat attggctaat acccaatttt tctgaatcta aaaagcgcct
    19141 gcgggtggtt ttagtgggag attaacatat tgaaaataaa gattttttat tttcatgtgt
    19201 ccagataacg tccatctttg aaacaaaaag cccgcagttt ttttacgccg cgggctttgt
    19261 ttttctttgt gacttttgtg tgagggtgcg actttcgacc gaacctttgg caaccggata
    19321 gcgggggctt tcacccccgc tgcggtttct tacggtttac actgcaagaa cgccgcaaat
    19381 tctgaccaca tgatgttaag tctgaactca cacggagaac ggtgtatcat ccagacggtc
    19441 aacaacatgg ttaagcataa aatgctcacg atgatcgttt tttgcggcat agcgcttgac
    19501 tccttttaca gggaggcgct aaccttccac ttgtctaggg taggaagggt taggcctcgg
    19561 gttaaacaaa tgtttgactc ggggcctttc cacatccggc cttcaggtaa tccttccagc
    19621 cgtcagccga aaggcacccg cgcgtaattt accttttacg tccgttacgt gcaacggatg
    19681 acgtggctgt ttttccttcc ccttcgccat cctttttcgg agcagttgtg
//
LOCUS       DAZFLD010000081        19661 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.081, whole genome shotgun sequence.
ACCESSION   DAZFLD010000081 DAZFLD010000000
VERSION     DAZFLD010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19661)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19661
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.081"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            221..3484
                     /locus_tag="ACHKBL_002452"
     CDS             221..3484
                     /locus_tag="ACHKBL_002452"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009035 - type I site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HsdR family type I site-specific
                     deoxyribonuclease"
                     /protein_id="HFO5819310.1"
                     /translation="MSLSFSEAKLEQAIIELLQDQGYQHLIGDNVPRSSLDQVIIEDD
                     LRHYLAARYQPDGITEEEIQRLIKQFTTLPASDLYESNKTFCRWLANGFLFKRDDRQQ
                     KDLYIELLDTRHLPAALRQIFDAEDVPLQQAAELPPSYINPPLNRIKIVNQLKISGKD
                     NQTRIPDGILYINGLPLVVFEFKSAVREQEANIGDAWKQLCKRYRRDIPQLFIYNALC
                     IISDGVNNRMGNLFAPYEYFYSWRKVTGNENREQDGIPSLHSMIQGLFHPVRLLDVIK
                     NFICFPDKAKHEVKICCRYPQYYAARKLYYSIKQARKPFGSGKGGTYFGATGCGKSYT
                     MQFLTRLLMKSVEFASPTIVLITDRTDLDDQLSAQMCNAKNYIGDDTIVPVTSREDLR
                     NQLAGRNSGGVFLTTIHKFTEDTELLSERSNIICISDEAHRSQVNLDQKVIVDKESGK
                     VRKTYGFAKYLHDSLPNATYVGFTGTPIDATLDVFGEVIDSYTMTESVQDEITVRIVY
                     EGRAAKVILDSSKLEEVEKYYEECANAGTNEWQIDESKKATATMNAVLGDEDRLKALA
                     EDFAKHYEKRVAEGSTVIGKAMFVCASREIAWDFYRQLKAIRPAWFEVKQAPDGVVLT
                     EQEQKELPPSEMVKMVMTRGKDDDEALYDLLGTKEYRKELDKQFKNAKSNFKIAIVVD
                     MWLTGFDVPELDTIYIDKPLQKHNLIQTISRVNRKLEGKSKGLVVDYIGIKSQMNQAL
                     AMYSRIDATNFEDIQQSVIEVKNHLDLLGQVFYEFDSRDYFSGEPQAQLSCLNRAAEF
                     VLRTQKVERRFMGLVKRMKAAYDVCCGSEALSQTERDHIHYYLAVRSIVFKLTKGDAP
                     DVTQMNARVREMIAEALKADGVEEIYFLGDKKAESIDIFDEDYLARINKIKLPATKIQ
                     LLQKLLEKAISDFKKVNQLQGINFTRRFQAIIDRYNERREDDVLNGEEFDTFSQEMTD
                     IIYDIKTEMGTWADLGIDIEEKAFFDILAHMRDKYQFTYDDEKMLSLAKEMKSVVDNT
                     SKYPDWSKRDDIKAKLKVELILLLHKHKFPPVANDDVYMGVLAQAENFKEHHMSALN"
     gene            3699..4037
                     /gene="symE"
                     /locus_tag="ACHKBL_002453"
     CDS             3699..4037
                     /gene="symE"
                     /locus_tag="ACHKBL_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001534835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease SymE"
                     /protein_id="HFO5819311.1"
                     /translation="MTDTHSIAQPFEAEVSPANNRQLTVSYASRYPDYSRIPAITLKG
                     QWLETAGFATGTAVDVKVIEGCIVLTAQPPAAEESELMQSLRQVCKLSARKQRQVQDF
                     IGVISSKTPR"
     gene            complement(4079..4132)
                     /locus_tag="ACHKBL_002454"
     CDS             complement(4079..4132)
                     /locus_tag="ACHKBL_002454"
                     /inference="COORDINATES: protein motif:HMM:NF018294.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1127 domain-containing protein"
                     /protein_id="HFO5819312.1"
                     /translation="MSDERLRDIGLRREDVE"
     gene            complement(4121..4276)
                     /locus_tag="ACHKBL_002455"
                     /pseudo
     CDS             complement(4121..4276)
                     /locus_tag="ACHKBL_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001684533.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytoplasmic protein"
     gene            4419..5831
                     /gene="yjiR"
                     /locus_tag="ACHKBL_002456"
     CDS             4419..5831
                     /gene="yjiR"
                     /locus_tag="ACHKBL_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFO5819313.1"
                     /translation="MTRYQHLATLLAERIEQGLYRHGEKLPSVRSLSQEHGVSISTVQ
                     QAYQTLETMKLITPQPRSGYFVAQRKAQPPVPPMTRPVQRPVEITQWDQVLDMLEAHS
                     DSSIVPLSKSTPDVETPSLKPLWRELSRVVQHNLQTVLGYDLLAGQRVLREQIARLML
                     DSGSVVTADDIIITSGCHNSMSLALMAVCKPGDIVAVESPCYYGSMQMLRGMGVKVIE
                     IPTDPETGISVEALELALEQWPIKGIILVPNCNNPLGFIMPDARKRAVLSLAQRHDIV
                     IFEDDVYGELATEYPRPRTIHSWDIDGRVLLCSSFSKSIAPGLRVGWVAPGRYHDKLL
                     HMKYAISSFNVPSTQMAAATFVLEGHYHRHIRRMRQIYQRNLALYTCWIREYFPCEIC
                     ITRPKGGFLLWIELPEQVDMVCVARQLCRMKIQVAAGSIFSASGKYRNCLRINCALPL
                     SETYREALKQIGEAVYQAME"
     gene            complement(6073..7017)
                     /locus_tag="ACHKBL_002457"
     CDS             complement(6073..7017)
                     /locus_tag="ACHKBL_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001678496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HFO5819314.1"
                     /translation="MTNFTTSTPHDALFKSFLTHPDTARDFMEIHLPKDLRELCDLDS
                     LKLESASFVDEKLRALHSDILWSVKTREGDGYIYVVIEHQSREDIHMAFRLMRYSMAV
                     MQRHIEHDKRRPLPLVIPMLFYHGSRSPYPWSLCWLDEFADPTTARKLYTAAFPLVDV
                     TVVPDDEIVQHRRVALLELIQKHIRQRDLMGLIDQLVILLVTECANDSQITALLNYIL
                     LTGDEARFNEFISELTRRMPQQRERIMTIAERIHNDGWLLGMEKGKEEGEQRLLRLLL
                     QNGADPEWIQRYTGLSAEQMQALEQPLPESKRDPWIEY"
     gene            7484..8716
                     /gene="mdtM"
                     /locus_tag="ACHKBL_002458"
                     /pseudo
     CDS             7484..8716
                     /gene="mdtM"
                     /locus_tag="ACHKBL_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313327.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtM"
     gene            8757..10037
                     /gene="yjiN"
                     /locus_tag="ACHKBL_002459"
     CDS             8757..10037
                     /gene="yjiN"
                     /locus_tag="ACHKBL_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 domain-containing protein"
                     /protein_id="HFO5819315.1"
                     /translation="MNKLIELRRAKMLALSLLLIAAATFVVTLFLPPNFWVSGVKAIA
                     EAAMVGALADWFAVVALFRRVPIPIISRHTAIIPRNKDRIGENLGQFVQEKFLDTQSL
                     VALIRRHEPALLIGNWFSQPENARRVGQHLLQIMSGFLELTDDARIQRLLKRAVHRAI
                     DKVDLSGTSALMLESMTKNDRHQVLLDTLIAQLIALLQRDKSRKFIAQQIVRWLESEH
                     PLKAKILPTEWLGEHSAELVSDAVNSLLDDISRDRAHQIRHAFDRATFALIDKLKNDP
                     EMAARADAVKSYLKEDEAFNRYLSELWGDLREWLKVDINSEDSRVKERIARAGQWFGE
                     TLIADDALRASLNGHLEQAAHRVAPEFSAFLTRHISDTVKSWDAQDMSRQIELNIGKD
                     LQFIRVNGTLVGGCIGLILYLLSQLPALFPLGNL"
     gene            10153..11304
                     /gene="yjiM"
                     /locus_tag="ACHKBL_002460"
     CDS             10153..11304
                     /gene="yjiM"
                     /locus_tag="ACHKBL_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="double-cubane-cluster-containing anaerobic
                     reductase"
                     /protein_id="HFO5819316.1"
                     /translation="MSLVTDLPAIFDQFSEARQKGFLTVMDLKERGIPLVGTYCTFMP
                     QEIPMAAGAVVVSLCSTSDETIEEAEKDLPRNLCPLIKSSYGFGKTDKCPYFYFSDLV
                     VGETTCDGKKKMYEYMAEFKPVHVMQLPNSVKDDASRALWKAEMLRLQKTVEERFGHE
                     ISEDALRDAIALKNRERRALANFYHLGQLNPPALSGSDILKVVYGATFRFDKEALINE
                     LDAMTARVRQQWEEGQRLDPRPRILITGCPIGGAAEKVVRAIEENGGWVVGYENCTGA
                     KATEQCVAETGDVYDALADKYLAIGCSCVSPNDQRLQMLSQMVEEYQVDGVVDVILQA
                     CHTYAVESLAIKRHVRQQHNIPYIAIETDYSTSDVGQLSTRVAAFIEML"
     gene            11314..12081
                     /gene="yjiL"
                     /locus_tag="ACHKBL_002461"
     CDS             11314..12081
                     /gene="yjiL"
                     /locus_tag="ACHKBL_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001298028.1"
                     /note="YjiL, as found in Escherichia coli, is a homolog of
                     the activator ATPases of enzymes such as lactoyl-CoA
                     dehydratase, (R)-phenyllactate dehydratase, and
                     2-hydroxyisocaproyl-CoA dehydratase. The typical substrate
                     of those enzymes is acyl-CoA with an OH at the
                     beta-position. YjiL is the putative activator for the
                     cognate protein YjiM, a putative 2-hydroxyacyl-CoA
                     dehydratase with unknown specificity, encoded by the
                     adjacent gene; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-hydroxyacyl-CoA dehydratase activator
                     YjiL"
                     /protein_id="HFO5819317.1"
                     /translation="MTYSIGIDSGSTATKGILLADGVITRRFLVPTSFRPATAITEAW
                     ETLREGLETTPFLTLTGYGRQLVDFADKQVTEISCHGLGARFLAPATRAVIDIGGQDS
                     KVIQLDDDGNLCDFLMNDKCAAGTGRFLEVISRTLGTSVEQLDSITENVTPHAITSMC
                     TVFAESEVISLRSAGVAPEAILAGVINAMARRSANFIARLSCEAPILFTGGVSHCQRF
                     THMLESHLGMPVQTHPDAQFAGAIGAAVIGQRQRKRA"
     gene            12078..12335
                     /gene="ytiA"
                     /locus_tag="ACHKBL_002462"
     CDS             12078..12335
                     /gene="ytiA"
                     /locus_tag="ACHKBL_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HFO5819318.1"
                     /translation="MKEHLFLFHSTVGVIQTRKALQAAGMTFRVSDIPRDLRGGCGLC
                     IWLTCPPGEEIQWVIPGHTESVYCQQDGGWRCIAHYGISPR"
     gene            12400..13260
                     /gene="yjiK"
                     /locus_tag="ACHKBL_002463"
     CDS             12400..13260
                     /gene="yjiK"
                     /locus_tag="ACHKBL_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418753.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdiA-regulated domain-containing protein"
                     /protein_id="HFO5819319.1"
                     /translation="MTKSISLSKRIFVIVILFVIVAVCTFFVQSCARKSNHAASFQNY
                     HATIDGKEIAGITNNISSLTWSAQSNTLFSTINKPAAIVEMTTNGDLIRTIPLDFVKD
                     LETIEYIGDNQFVISDERDYAIYVISLTPNSEVKILKKIKIPLQESPTNCGFEGLAYS
                     RQDHTFWFFKEKNPIEVYKVNGLLSSNELHISKDKALQRQFTLDDVSGAEFNQQKNTL
                     LVLSHESRALQEVTLVGEVIGEMSLTKGSRGLSHNIKQAEGVAMDASGNIYIVSEPNR
                     FYRFTPQSSH"
     gene            13328..14506
                     /gene="yjiJ"
                     /locus_tag="ACHKBL_002464"
     CDS             13328..14506
                     /gene="yjiJ"
                     /locus_tag="ACHKBL_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO5819320.1"
                     /translation="MPSSTHPVERFSFSTALFGMLVLTLGMGLGRFLYTPMLPVMMAE
                     GSFSFSQLSWIASGNYAGYLAGSLLFSFGAFHQPSRLRPFLLASALASGLLILAMAWL
                     PPFILVLLIRVLAGVASAGMLIFGSTLIMQHTRHPFVLAALFSGVGIGIALGNEYVLA
                     GLHFDLSSQTLWQGAGALSGMILIALTLLMPSKKHAIVPMPLAKTEQQIMSWWLLAIL
                     YGLAGFGYIIVATYLPLMAKDAGSPLLTAHLWTLVGLSIVPGCFGWLWAAKRWGALPC
                     LTANLLVQAICVLLTLASDSPLLLIISSLGFGGTFMGTTSLVMTIARQLSVPGNLNLL
                     GFVTLIYGIGQILGPALTSMLGNGTSALASATLCGAAALFIAALISTVQLFKLQVVTS
                     "
     gene            complement(14519..15073)
                     /gene="kptA"
                     /locus_tag="ACHKBL_002465"
     CDS             complement(14519..15073)
                     /gene="kptA"
                     /locus_tag="ACHKBL_002465"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
                     /protein_id="HFO5819321.1"
                     /translation="MAKYNDKELAETSKFLSFVLRHKPEAIGIVLDREGWADIDKLIL
                     CAQKAGKRLTRALLDIVVATSDKKRFSYSSDGTCIRAVQGHSTSQVAIGFIEKTPPQF
                     LYHGTASRFLDEIKKQGLIAGERHYVHLSADEATARKVGARHGSPVILTVKAQEMAKR
                     GIPFWQAENGVWLTSTVAVEFLEW"
     gene            15323..16006
                     /gene="yjiH"
                     /locus_tag="ACHKBL_002466"
     CDS             15323..16006
                     /gene="yjiH"
                     /locus_tag="ACHKBL_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HFO5819322.1"
                     /translation="MTQQGDAVAGELATEKVGIKGYLAFFLTIIFFSGVFSGTDSWWR
                     VFDFSVLNGSFGQLPGANGATTSFRGAGGTGAKDGFLFALELAPSVILSLGIISITDG
                     LGGLRAAQQLMTPVLKPLLGIPGICSLALIANLQNTDAAAGMTKELAQEGEITERDKV
                     IFAAYQTSGSAIITNYFSSGVAVFAFLGTSVIVPLAVILVFKFVGANILRVWLNFEER
                     RNPTQGAQA"
     gene            16003..16464
                     /gene="yjiG"
                     /locus_tag="ACHKBL_002467"
     CDS             16003..16464
                     /gene="yjiG"
                     /locus_tag="ACHKBL_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HFO5819323.1"
                     /translation="MTTQVRKNVMDMFIDGARRGFTIATTNLLPNVVMAFVIIQALKI
                     TGLLDWVGHICEPVMALWGLPGEAATVLLAALMSMGGAVGVAASLATAGALTGHDVTV
                     LLPAMYLMGNPVQNVGRCLGTAEVNAKYYPHIITVCVINALLSIWVMQLIV"
     gene            16477..17649
                     /gene="iadA"
                     /locus_tag="ACHKBL_002468"
     CDS             16477..17649
                     /gene="iadA"
                     /locus_tag="ACHKBL_002468"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008798 - beta-aspartyl-peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HFO5819324.1"
                     /translation="MIDYSAAGFTLLQGAHLYAPEDRGICDVLIANGKIIAVASNIPS
                     DIVPDCTVVDLSGQILCPGFIDQHVHLIGGGGEAGPTTRTPEVALSRLTEAGVTSVVG
                     LLGTDSISRHPESLLAKTRALNEEGISAWMLTGAYHVPSRTITGSVEKDVAIIDRVIG
                     VKCAISDHRSAAPDVYHLANMAAESRVGGLLGGKPGVTVFHMGDSKKALQPVYDLLEN
                     CDVPISKLLPTHVNRNVPLFEQALEFARKGGTIDITSSIDEPVAPAEGIARAVQAGIP
                     LARVTLSSDGNGSQPFFDDEGNLTHIGVAGFETLLETVQMLVKDYSFSLSDALRPLTS
                     SVAGFLNLTGKGEILPGNDADLLVMTPELRIEQVYARGKLMVKDGKACVKGTFETA"
     gene            17714..18625
                     /gene="hypT"
                     /locus_tag="ACHKBL_002469"
                     /gene_synonym="qseD"
     CDS             17714..18625
                     /gene="hypT"
                     /locus_tag="ACHKBL_002469"
                     /gene_synonym="qseD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001677288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypochlorite stress DNA-binding transcriptional
                     regulator HypT"
                     /protein_id="HFO5819325.1"
                     /translation="MDDCGAVLHNIETKWLYDFLTLEKCRNFSQAAVSRNVSQPAFSR
                     RIRALEQAIGVELFNRQVTPLQLSEQGKIFHSQIRHLLQQLESNLAELRGGSDYAQRK
                     IKIAAAHSLSLGLLPSIISQMPPLFTWAIEAIDVDEAVDKLREGQSDCIFSFHDEDLL
                     EAPFDHIRLFESQLFPVCASDEHGEALFNLAQPHFPLLNYSRNSYMGRLINRTLTRHS
                     ELSFSTFFVSSMSELLKQVALDGCGIAWLPEYAIQQEIRSGQLVVLNREELVIPIQAY
                     AYRMNTRMNPIAERFWRELRELEIEPG"
     gene            18632..19003
                     /locus_tag="ACHKBL_002470"
                     /pseudo
     CDS             18632..19003
                     /locus_tag="ACHKBL_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001519422.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative PEP-binding protein"
     gene            complement(19051..19434)
                     /gene="iraD"
                     /locus_tag="ACHKBL_002471"
     CDS             complement(19051..19434)
                     /gene="iraD"
                     /locus_tag="ACHKBL_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001695400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraD"
                     /protein_id="HFO5819326.1"
                     /translation="MMRQSVQTVLPESTGHNTLSLRDSVCRDLFQLFSSPHSPLPILL
                     VSGMPEWQGHNQSDKLLQSWYCRQLRSALLFHEPRIAALQVNLKEAYCHELAISLEMM
                     LYHDDEPLTFDLVWQKGNWHRTMPQ"
BASE COUNT         4703 a         4952 c         4947 g         5059 t
ORIGIN      
        1 ttttctaatt cgtctgaaaa taatacccta acagccctcc gcaataccct gctccccaaa
       61 ctcatctccg gcgagctatc cgtggaggat ctcccggatc acatcaaaca aaccgaagcc
      121 gcataacgca ttttgcccct gtaaaatcag gggctttctg gtaaggtttt ctactgatac
      181 gggaatgctt accagaaatt agccagggtt ggagcacgat atgagtctct ctttcagtga
      241 agcaaaatta gaacaagcga tcattgaact gttacaggat cagggatatc aacacctgat
      301 cggcgataat gtcccacgtt caagtctcga tcaggtcatt atcgaagacg atctccgtca
      361 ttatttagcg gcacgctacc agcctgatgg cattactgaa gaagagattc agcgactgat
      421 caaacagttc accacgcttc cggcctccga tctttatgaa agcaacaaaa cattttgtcg
      481 ctggctggca aatggttttc tgttcaaacg cgacgatcgg caacaaaaag atctctatat
      541 tgaattgctc gacacccggc atctacctgc cgcactgcgc cagatatttg acgccgaaga
      601 tgtcccgttg caacaggctg cggaactccc gccttcctat attaacccgc cgcttaaccg
      661 gattaagatt gttaaccagc ttaaaatctc cggcaaagat aatcagactc gtattcctga
      721 cggcattctc tatatcaacg gtctgccact ggtcgtcttt gaatttaaaa gcgcggtgcg
      781 cgagcaggaa gccaatatag gcgatgcctg gaagcagctc tgtaaacgct accgccggga
      841 tattccgcaa ctgtttatct acaacgcgct ctgcattatt agcgatggcg taaataaccg
      901 gatgggcaac ctgtttgcac cctatgaata tttttactca tggcgcaaag tcaccggtaa
      961 tgaaaaccgt gaacaggacg gaattccatc attgcactca atgattcagg ggctgtttca
     1021 tccggtacgt ctgctggatg tgattaaaaa ctttatctgc ttcccggata aagccaagca
     1081 cgaagtaaaa atttgctgcc gatatccgca gtactatgcc gcccgcaaac tctattacag
     1141 catcaagcaa gcgcgtaaac ctttcggtag cggtaaaggc ggtacctact ttggcgcaac
     1201 gggctgcggt aaaagctaca ccatgcaatt tttaacccgc ttgttaatga aaagcgtgga
     1261 gtttgccagc ccgaccattg ttttgatcac cgaccgcacc gatctggacg atcagctttc
     1321 tgcgcaaatg tgcaacgcca aaaattacat tggcgacgac accatcgttc ctgttaccag
     1381 ccgtgaagat ttgcgtaatc aactggcggg acgcaatagt ggcggtgtat tcctgacaac
     1441 gatccataaa ttcaccgaag acaccgaact cctttctgaa cgcagcaata tcatttgcat
     1501 ctcggacgaa gcacatcgca gccaggttaa cctcgaccag aaagtcatcg tcgataaaga
     1561 aagcggaaaa gtgcgcaaaa cgtatggctt tgcgaaatac ctgcacgatt cactgccaaa
     1621 tgccacctat gttggcttta ccggcacacc gattgacgcg acgctcgatg tcttcggtga
     1681 ggtgatcgac agttacacca tgaccgaatc cgttcaggat gaaatcactg tacgcatcgt
     1741 gtacgaaggc cgtgcggcta aagtgatcct ggactccagc aaactggaag aagtcgaaaa
     1801 gtattacgaa gagtgcgcaa acgcaggcac caatgagtgg caaatcgacg aaagcaagaa
     1861 agccacggct accatgaatg cggttctggg tgatgaagat cggttaaaag ccctcgcgga
     1921 agattttgct aaacattatg aaaaacgcgt agccgaaggt tccaccgtaa taggcaaagc
     1981 catgtttgtt tgtgccagcc gtgaaattgc ctgggatttc taccgccagc ttaaagctat
     2041 tcgccctgcc tggtttgaag tgaagcaagc acctgatggc gtcgtcctga cggaacagga
     2101 acaaaaagag ttaccgcctt ctgaaatggt gaaaatggtc atgacgcgcg gtaaagatga
     2161 cgacgaggcg ctttatgatt tactgggcac gaaagaatat cgtaaagagc tggataagca
     2221 gttcaaaaac gctaaatcga atttcaaaat tgccattgtc gttgatatgt ggctgaccgg
     2281 ttttgatgtt cctgaactgg ataccatcta tattgataag cccttacaaa aacataacct
     2341 tatccagact atttctcgcg ttaaccgtaa gctggaaggc aaaagcaaag ggttagtggt
     2401 ggactacatc ggcattaaaa gtcagatgaa ccaggcactg gcaatgtatt cccgcattga
     2461 tgccaccaac tttgaagata ttcagcaatc ggtaatagaa gttaaaaacc atctcgattt
     2521 gttggggcaa gtcttttacg aatttgacag tcgggattat tttagtggtg agccacaagc
     2581 gcaattatcc tgcctcaacc gcgcggcgga atttgttctg cgtacccaga aagttgaacg
     2641 tcgttttatg ggactggtta aacgcatgaa agccgcctac gacgtctgct gcggcagcga
     2701 agcattatca cagacagaac gtgatcatat tcactattat cttgctgttc gttcaattgt
     2761 tttcaaactg acgaaaggtg acgcaccgga tgtcacccag atgaatgcac gcgttcgtga
     2821 aatgattgca gaagcgctaa aagctgatgg cgtagaagaa atttattttc ttggcgataa
     2881 aaaagcggaa tccatcgata tttttgacga agattatctg gcgcgaatta acaagatcaa
     2941 acttccggca acaaagatcc agttattaca aaaattactg gaaaaagcga tcagcgactt
     3001 caagaaagtg aaccagttgc aagggattaa cttcacccgc cgcttccagg cgattataga
     3061 tcgttataat gagcggcgag aagatgatgt actcaacggt gaagaattcg atacattcag
     3121 tcaggaaatg accgatatta tctatgatat taaaaccgaa atgggcacct gggccgattt
     3181 aggcattgat attgaagaaa aagccttttt cgacattctt gctcatatgc gcgataaata
     3241 tcagttcacc tatgacgatg aaaaaatgct gtcactggca aaagagatga aaagcgtggt
     3301 tgacaacaca tcgaaatatc ctgactggag taaacgcgat gatattaaag cgaaactgaa
     3361 agttgaactt attctgcttc tacacaagca taagttcccg ccagtagcga atgatgatgt
     3421 ttatatgggt gtgctggcac aggcagagaa ttttaaagaa catcatatga gtgctttaaa
     3481 ttaattatga aggccggata cactttgtta tccggccatt ctgaattaac gttaatttct
     3541 cttccagatc tttccagtaa cgcttttatt attttctatt tttagctgga ctttccctgt
     3601 atttactgat gatatatcca ggtatttagc gcggtgcgaa tgtgcgccaa cacacccgca
     3661 ccgctcatca caatccctat ttcacgagaa tagtaatcat gactgacacg cattctattg
     3721 cacaaccgtt cgaagcagaa gtctccccgg caaataaccg tcaattaacc gtgagttatg
     3781 cgagtcgcta cccggattac agccgtattc ccgccatcac cctgaaaggt cagtggctgg
     3841 aaaccgccgg ttttgccacc ggcacggcgg tagatgtcaa agtgatagaa ggctgtattg
     3901 tcctcaccgc ccaaccaccc gccgccgaag agagcgaact gatgcagtca ctgcgccagg
     3961 tgtgcaagct gtcggcgcgt aaacaaaggc aggtgcagga ttttattggg gtgattagta
     4021 gcaaaacgcc acgctaagtt tctctcccct tccccatacg ggaagggaaa ttgccccctc
     4081 actccacatc ctccctgcgt aacccgatat ccctcaaccg ttcatcactc atctgctgta
     4141 cacccgccgg gcctgcattt gccgccgcca acgcctcacc gcctgtcaga gttgcacaag
     4201 tctgataaac ggcgttttgg ctttgttttc gtgaaattcc atctgctgcc tcctcatctt
     4261 tcagaggctt catcatccgt cttctggcag ttcacaatac agatgcaaat attagtttta
     4321 tttaacatac agacaggtta aaacagcatc tgcatccggt tttttagcct catctgtacc
     4381 ggtttttctc tctgtatggt ccaaataagg aatacagcat gacgcgttat caacatctgg
     4441 cgactctact tgccgagcgg attgagcaag ggctgtatcg tcacggggag aaattgccgt
     4501 cggtgcgcag cttaagtcag gagcacggcg tcagtattag caccgtgcag caggcgtacc
     4561 agacgctgga gacgatgaag ctcatcactc cgcagccgcg ttcgggttat tttgtcgcac
     4621 aacgtaaagc ccagccgcca gtaccgccga tgacgcgtcc ggtgcagcgc ccggtggaaa
     4681 ttacccagtg ggatcaagtg ctggatatgc tggaggcgca tagcgacagt tccattgttc
     4741 cgttaagtaa aagcacgccg gatgtcgaaa cgcccagcct gaaaccgctg tggcgggagc
     4801 taagccgggt ggtgcagcat aatctgcaaa ccgttctcgg ttatgacttg ttagccggtc
     4861 agcgagtgtt gcgagagcag attgcccgcc tgatgctcga cagcggctca gtggtcaccg
     4921 ctgatgacat cattatcacc agcggctgcc ataactcgat gtcgctggcg ttaatggcgg
     4981 tatgtaaacc gggcgatatt gtcgcagtcg aatccccctg ttattacggt tcaatgcaga
     5041 tgctgcgcgg tatgggcgtg aaagtgattg aaatcccaac cgatccagaa actggcatca
     5101 gcgttgaagc gctggaactg gcgctggaac agtggccgat taaaggcatc attctggtgc
     5161 caaactgtaa taatccgctg ggatttatta tgccggacgc acgcaaacgg gccgttctct
     5221 ctctcgctca gcgtcatgat attgtgattt ttgaagatga tgtctacggc gaactggcga
     5281 cggagtatcc gcgcccgcgg accattcatt cctgggatat cgacgggcga gtgctgctgt
     5341 gcagctcgtt cagtaaaagt attgcaccag gcctgcgcgt gggttgggtc gcaccggggc
     5401 gttatcacga taaactgctg catatgaaat atgccatcag cagctttaat gtaccgtcca
     5461 cgcaaatggc ggcggcaacg tttgttctgg aaggtcacta tcatcgccat atccggcgga
     5521 tgcggcagat ctatcagcgc aatttggcgc tttatacctg ctggatacgg gaatattttc
     5581 cctgcgaaat ctgtattacg cgcccgaaag gcggattttt actgtggata gaattgcctg
     5641 aacaggtcga tatggtttgc gttgcgcggc agctgtgccg catgaaaatc caggtggcgg
     5701 caggctcgat tttctcagct tccggcaaat accgtaattg tctgcgcatc aactgcgctt
     5761 tgccgctcag cgaaacctat cgcgaagcac tgaagcaaat tggcgaggcc gtgtatcagg
     5821 caatggaatg aattcgaatt agtggcgagg cccctgattt cctctcacga atcgcctgtg
     5881 actcgtttta tctttgcagg ggcatcgcca cgctccagca tggcttaggc aaagtgtgct
     5941 ttgttcatac cggatgcggc gtgaacgcct tatccggctt acaaatcgcg caaattcaat
     6001 agattgcaga ggtaatgtag gtctgataag cgtagcacat caggcagctt tgcgtttgtc
     6061 atcagtctct gattagtact cgatccatgg atcgcgcttg ctttcaggca agggttgctc
     6121 taatgcctgc atttgctctg ccgaaagtcc agtatatcgt tgtatccatt caggatccgc
     6181 accgttctgc aaaagcaatc taaggaggcg ttgttcccct tcttctttcc ccttttccat
     6241 ccctaacagc catccatcat tatgaattcg ctctgcaatc gtcattattc gctccctttg
     6301 ttgtggcatt cgacgggtaa gttcactgat aaactcatta aaacgcgctt catcgccagt
     6361 cagtaaaatg taatttaaca gcgcagttat ctggctgtca ttagcacact ctgtaaccag
     6421 taatattacc agttgatcga taagccccat cagatcgcgc tggcgaatat gcttttggat
     6481 caactccaac agggcgactc tgcgatgctg cacaatctcg tcgtctggca cgacagtgac
     6541 atccaccagc gggaacgctg cggtataaag cttccgtgcg gtggtcgggt cagcaaattc
     6601 gtccagccag cacagggacc agggataagg actacggcta ccgtgataaa acagcatcgg
     6661 gatgaccagc ggtagcggtc ggcgtttatc atgctcgata tggcgctgca tcaccgccat
     6721 ggaatatcgc atcaggcgaa aggccatatg gatatcctcg cggctctgat gttcaatcac
     6781 tacgtaaata taaccatcac cttcacgggt ctttaccgac cacagaatat cggagtgtag
     6841 cgcccgcaat ttttcatcga cgaagctggc ggattccagt tttaagctgt cgagatcgca
     6901 cagttcacgt aaatctttgg gtaagtggat ctccataaaa tcccgcgcgg tgtcagggtg
     6961 cgtgagaaag gatttaaata atgcgtcatg cggcgtactg gtcgtgaagt ttgtcatggc
     7021 gatccgtcac ctgttgataa gatggcccga ctttagcgac taacaaaacc attctcaccc
     7081 ggtaatttcc cattccccga ggggctttcc aggctatttt tattcttctg caaacgatac
     7141 atttttccgg aaacaggctc gcattttaca tttcctgtac ccggcagata ttcccctttc
     7201 caccgcactc ttttgattaa ccgcaaattt taattacact taaggtgtat attttctatg
     7261 caacccatca attcaagagg tgtaatgtgc tgatgactat ttgtaatcgt tatacattct
     7321 gacctggagt cagaaggtat ttctctgtct gtgtattcac aggcagtgtg gttgattaca
     7381 tgaaattcag tacatttatc cttctcgcct gcctttcgtc attaccgacg gtattgaatt
     7441 tcgttttccc cattggggtt ctccggacaa ggagttgttt gttatgccac gtttttttgc
     7501 ccgccatgcc gccacgctgt ttttcccgat ggcgttgatt ttgtatgact ttgctgcgta
     7561 tctgtcgacg gacctgatcc agccagggat cattaatgtg gtccgtgatt ttaatgccga
     7621 tgtcagtctg gcccctgctg ccgtcagtct ctatcttgct ggcggtatgg cgttacagtg
     7681 gctgctgggg ccgctttccg acagaattgg ccgcaggccg gtgctgatta ccggggcgtt
     7741 aatttttacc cttgcctgcg ccgcgacaat gttcaccacg tcaatgacac aatttcttat
     7801 cgcgcgtgca attcagggca ccagtatctg ttttattgcc accgttggtt atgtcacggt
     7861 gcaggaggcg tttggacaga caaaagggat caagttgatg gcgattatca cctccatcgt
     7921 actgattgcg ccgattatcg gcccgctttc cggcgcagct ctgatgcact ttgtgcactg
     7981 gaaagtcctt tttgccatca ttgcggttat gggttttatc tcatttgttg gcttactgct
     8041 ggcgatgcca gagacggtga agcgcggcgc ggttccattt agcgccaaaa gcgtcttgcg
     8101 cgattttcgt aatgtctttt gcaatcggct gttcctcttt ggcgcagcaa ccatctcttt
     8161 aagctatatc ccgatgatga gttgggtggc tgtctcgccg gtgatcctga ttgacgcagg
     8221 cggcttaacg acctcgcagt tcgcctgaac acaggttccg gtgttcggcg cggtgattgt
     8281 cgcgaatgcc atcgtggcgc gtttcgttaa agatccgacc gaaccgcgct ttatctggcg
     8341 cgccgtaccc cttcaactgg ttggcctcgc gctgttgatt atcggcaatc tgctgtcacc
     8401 gcacgtctgg ctgtggtcgg tgctgggcac cagtctgtat gctttcggga ttggtttgat
     8461 tttcccgacc ttattccgct ttacgctgtt ttccaataac ttaccgaaag ggaccgtctc
     8521 cgcatcgctg aatatggtga tcctgatggt gatgtctgtc tcggtcgaaa tcggccggtg
     8581 gctgtggttt aacggcggtc gcttgccgtt tcatctgtta gccgtcgtgg cgggcgttat
     8641 cgtcgttttc accctggcgg gattgctcaa tcgcgtgcgc caacatcagg ctgccgagct
     8701 ggcggaggag cggtgatttt tgcgcgatcc cgccgtcagg ctctattctt aacattatga
     8761 ataaactcat tgaactcaga cgcgccaaaa tgttggcgct ctctttactg cttatcgccg
     8821 ctgctacctt tgtcgttacg ctgtttttgc cgcccaattt ttgggtgagc ggcgtgaagg
     8881 cgattgctga agcggcgatg gtcggcgcgc tggcggactg gtttgcggtg gtggcgctgt
     8941 ttcgccgcgt gccgattccg atcatttctc gtcatacggc gattatcccg cgtaataaag
     9001 accggattgg cgaaaatctc ggtcagttcg tgcaggaaaa atttctcgat acccagtcgc
     9061 tggtggcatt gattcgacgt cacgaaccgg cgttgttgat tggcaactgg tttagtcagc
     9121 cggagaacgc ccgccgcgtt ggtcagcatc tgttgcagat catgagcggt tttcttgaac
     9181 tgaccgatga tgcgcgtatt cagcgcctgc ttaagcgcgc ggtccaccgg gcgattgata
     9241 aggttgatct ttctggcacc agtgcgttga tgctggagag tatgaccaaa aacgatcgtc
     9301 atcaggtgct gctcgatacg ctgatcgcac agttgatcgc ccttctccag cgcgataaat
     9361 cgcgcaagtt tatcgcccag caaattgttc gctggctgga gagcgagcat ccactgaaag
     9421 ccaaaatttt gcccactgaa tggctgggcg aacatagcgc ggagttggtt tctgacgcgg
     9481 tgaattcttt gcttgatgat atcagccgcg atcgtgcgca tcagatccgc catgcgtttg
     9541 atcgcgccac ctttgccctg atcgacaagc tgaaaaacga tcctgaaatg gcagcgcgag
     9601 ccgatgccgt aaaaagctat ctgaaagaag acgaagcttt taaccgctat ctcagtgaat
     9661 tgtgggggga tttacgggaa tggctgaaag tggatatcaa cagcgaagat tctcgtgtga
     9721 aagaacgtat cgcacgagcg ggtcaatggt ttggcgaaac gttaattgcc gatgatgcct
     9781 tgcgggcgtc gttaaatggt catctcgaac aagccgcgca ccgcgtcgcg cctgagtttt
     9841 ccgcattcct gacgcgccac atcagcgata cggtaaaaag ctgggatgcg caggatatgt
     9901 cgcggcaaat cgagttaaat atcggcaaag atctgcagtt tatccgtgtc aacggtacgc
     9961 tggttggcgg ttgtattgga cttattttgt atttgctgtc gcagctcccg gccttgttcc
    10021 ccctcggcaa tttatagaaa tccatgaaaa actaagtggc taaccgcgag agagatcaaa
    10081 taaaccacac taaaagaatg gtaataatgt ggcctctttt cattatctcc cgtggtacgg
    10141 ggaaggaaaa tcatgtcact tgtcaccgat ctacccgcca ttttcgatca gttctctgaa
    10201 gctcgccaga aaggctttct caccgtcatg gatctcaagg agcgcggcat tccgctggtt
    10261 ggcacctatt gcacctttat gccgcaagag atcccgatgg cagccggtgc ggttgtggtt
    10321 tcgctatgtt ccacctctga tgaaaccatt gaagaagccg agaaagatct gccgcgcaac
    10381 ctctgcccgc tgattaaaag cagctacggc ttcggcaaaa ccgataaatg cccctacttc
    10441 tacttttctg atctggtggt cggtgaaacc acctgcgacg gcaaaaagaa aatgtatgaa
    10501 tacatggcgg agtttaagcc cgttcatgtg atgcaactgc caaacagcgt taaggacgat
    10561 gcctcgcgtg cattatggaa agccgagatg ctgcgcttgc aaaaaacggt agaagaacgt
    10621 tttgggcacg agattagcga agatgctctg cgcgatgcca ttgcgctgaa aaaccgcgaa
    10681 cgtcgcgcac tggccaactt ttatcatttg gggcagttaa atcctccggc gcttagcggc
    10741 agcgacattc tgaaagtagt ctacggcgca accttccggt tcgataaaga ggcgttgatc
    10801 aatgaactgg atgcaatgac cgcccgcgtt cgtcagcagt gggaagaagg ccagcgactg
    10861 gacccgcgcc cacgtatttt aatcaccggc tgcccaattg gcggcgcagc agagaaagtg
    10921 gtgcgcgcga ttgaagagaa tggcggctgg gttgtcggtt atgaaaactg caccggggcg
    10981 aaagccaccg agcaatgcgt ggcggaaacg ggtgatgtgt acgacgcgct ggcggataaa
    11041 tatctggcga ttggctgctc ctgtgtttcg ccgaacgatc agcgcctgca aatgctcagc
    11101 cagatggttg aagaatatca ggtcgatggc gtagttgatg tgattttgca ggcgtgccat
    11161 acctacgcgg tggaatcgct ggcgattaaa cgtcatgtgc gccagcagca caacattcct
    11221 tatatcgcta ttgaaacaga ctactccacc tcggatgtcg ggcagctcag cacccgtgtc
    11281 gcggccttta ttgagatgct gtaaggagtg gcagtgacat attcgattgg cattgattcc
    11341 ggctcaaccg ccaccaaagg gatcttactg gctgacggcg tgattacgcg ccgtttcctc
    11401 gttccaacat cctttcgccc ggcaacagca attactgaag cctgggaaac tctgcgcgaa
    11461 gggttagaga caacgccgtt tctgacgctc accggctacg gacggcaact ggtggatttt
    11521 gccgataaac aggtgacgga aatctcctgt cacgggctgg gcgcacggtt tcttgcgcca
    11581 gcaacgcgcg cagtaatcga catcggtggt caggacagca aagtgattca gcttgatgat
    11641 gacggtaacc tgtgcgattt cctgatgaat gacaaatgcg cggcgggcac cgggcgtttc
    11701 ctggaggtga tctcgcgcac gcttggcacc agcgtcgaac agctcgacag cattaccgaa
    11761 aatgtcacgc cgcacgccat cacgagtatg tgcacagtgt ttgctgaatc agaagtgatc
    11821 agcctgcgct ctgcgggcgt agcgccagaa gcgattctcg ccggagtgat taacgcgatg
    11881 gcgcggcgga gtgccaattt cattgctcgt ctctcctgtg aagcgccgat tctgtttact
    11941 ggtggcgtta gccactgtca aaggtttacc cacatgctgg aaagccatct gggaatgccg
    12001 gtacaaactc accctgatgc gcaattcgct ggtgcaattg gcgcggcggt gatcggtcag
    12061 cggcagagaa aacgcgcatg aaagagcatc tgtttttatt tcactccacg gtcggcgtca
    12121 tacaaacccg caaggcgtta caggcagcgg gcatgacttt tcgcgtcagc gatattcccc
    12181 gtgatttacg cggcggctgc gggttatgca tttggctgac ctgtccgcct ggcgaggaaa
    12241 ttcaatgggt gatccctggg catacggaat cagtttattg tcagcaggat ggtggctggc
    12301 gctgcattgc acattacggg atttctccgc gttaaggatt cattaagatc gctacgtata
    12361 cttctcgata tcttattgat ttgccttaaa gggattaccg tgacaaaaag tatttcatta
    12421 agtaaacgca tcttcgttat tgtgattctt tttgtcattg tggcagtgtg tactttcttc
    12481 gtgcaatctt gcgcccgtaa aagcaatcat gcagcatcat ttcagaatta ccacgccacc
    12541 attgacggca aagaaattgc aggcataacg aataatatct cttcgctgac ctggtcggca
    12601 caaagcaata ctctttttag cactatcaac aaacctgccg ccattgttga aatgaccacc
    12661 aatggtgatt tgatccgcac aattcctctg gattttgtta aggatctcga gacgatcgaa
    12721 tacattggcg ataatcagtt tgtcattagc gatgagcgcg attacgccat ttatgtgatt
    12781 tcactgaccc caaactcgga agtaaaaatc ctcaaaaaaa taaaaatccc gctgcaggaa
    12841 tccccaacca attgcggttt tgaaggattg gcctattctc gccaggatca cactttctgg
    12901 ttttttaaag agaaaaaccc gattgaggtc tacaaagtta acgggctatt aagtagcaat
    12961 gaattgcaca tcagcaaaga caaggcacta caacggcaat ttactttaga tgatgtgtcc
    13021 ggcgcagaat ttaaccaaca aaaaaatacg cttctggtgc tgtctcatga atctcgcgca
    13081 ctccaggaag taacgctggt tggcgaggtt atcggtgaaa tgtcactaac caaaggcagt
    13141 cgaggtttgt cgcataacat caaacaggcc gaaggggttg cgatggatgc ctctggtaat
    13201 atttatatcg ttagcgaacc aaatcgtttt tatcgcttta cccctcaatc atcgcattaa
    13261 caggctggca tgcttcacct ttttgttatt aacttgttaa caacaaaaat gacaaacaag
    13321 gttaaacatg ccttcgtcca cgcatcccgt agaacgcttt tctttcagca ccgcgctttt
    13381 cgggatgctg gttctgacct taggtatggg tttaggccgc ttcctttata cgcctatgtt
    13441 gcccgtcatg atggcggaag gatcgttttc atttagccag ctctcgtgga ttgccagcgg
    13501 caactatgcg gggtatctgg ctggcagtct gctattttct tttggcgcat ttcaccagcc
    13561 atcgcgcctg cgcccatttc tgttggcttc tgccctggcg agcgggttgt tgatcctcgc
    13621 aatggcatgg ttgccgccat ttattctggt gttattgatt cgcgtcctgg cgggtgtcgc
    13681 cagcgccgga atgctgattt ttggttcgac gctgattatg cagcacacgc gccatccttt
    13741 tgtgctggca gctttgtttt ctggcgttgg cattggcatc gcactgggca atgaatatgt
    13801 tctggcaggc ctgcattttg acctctcctc gcaaacgtta tggcaaggcg ccggggcgct
    13861 ttctggcatg atactgattg cacttacgct tttaatgccc tcgaaaaaac acgccatcgt
    13921 accaatgcca ttggcaaaaa cggagcaaca gataatgagc tggtggttac tggctattct
    13981 gtacggcctg gcgggatttg gttatatcat cgtcgcgact tacctgccgc tcatggcgaa
    14041 agatgcaggc tcaccattgt taaccgccca tctctggacg ttagtcggct tatcaattgt
    14101 gcctggttgc tttggctggc tatgggccgc aaaacgttgg ggggctttgc cctgcctgac
    14161 ggcgaatttg ctggtgcagg ctatctgtgt gctgcttact ctcgccagcg actcgcctct
    14221 cttgcttatc atcagcagtc ttggttttgg cggtaccttt atgggcacga catctctggt
    14281 gatgactatc gcccgccaac tgagcgtacc gggaaatctc aatcttttag gctttgtgac
    14341 gctcatttac ggcattgggc aaatcctcgg cccggcgctg accagtatgc tcggcaatgg
    14401 aacatcggcg cttgccagcg ccactctctg cggcgcggcg gcgttgttta ttgctgcgct
    14461 tatcagcaca gtgcaattat ttaagctaca agtggtcact tcgtgagaga agtttccctt
    14521 accactcaag aaactcaacc gcaactgttg atgtcagcca gaccccgttt tccgcttgcc
    14581 aaaaagggat gcctcgtttc gccatttcct gtgctttgac ggttaaaata actggcgatc
    14641 cgtgtcttgc tcccactttg cgtgccgttg cttcatcagc agagagatgc acgtaatggc
    14701 gctcccctgc aatcagccct tgttttttta tctcgtcaag aaaacggctt gccgtaccat
    14761 ggtagagaaa ttgtggcggt gttttttcga tgaagccaat ggcgacctgc gaagtcgaat
    14821 gcccctgaac ggcgcgaata cacgtaccat cactggaata actaaagcgc tttttatcgc
    14881 tggtagcgac aacgatatcc agtaatgcgc gggttaaccg cttgcccgct ttttgggcac
    14941 agagtatgag cttatcgata tccgcccatc cttcacggtc cagcacgatg ccaatcgcct
    15001 ctggtttatg tcggaggaca aaacttaaaa acttgctcgt ctctgccagc tctttgtcgt
    15061 tgtattttgc catctcgttc tcgtccctgg attttctgtt tttatttact gatgtcattg
    15121 tcactgggtt tatgaatttt ctgcaactca atcctgcgca gaacctttgc cacacagcgt
    15181 catttttccg ctatgcaaaa aacgcatagc cagaaacata cttgcatttt ccccataccc
    15241 acatggctga taaggttttt cagctccagg aattttcctg gctcaagcaa cacacaaaca
    15301 caaaaaaaca atggggattg ttatgacaca acaaggggat gctgtagccg gggagctggc
    15361 gacagaaaaa gtgggcatta aagggtatct ggcttttttt ctcaccataa tctttttttc
    15421 cggtgttttt tccgggacag acagttggtg gcgcgttttt gatttttccg tgctgaacgg
    15481 atcgtttggg caactgcctg gtgcgaatgg cgccaccacc tcgtttcgcg gcgcaggtgg
    15541 cacaggagct aaagatggct ttctcttcgc gctggaactg gcaccgtcag tcattctctc
    15601 tctggggatt atttcaatta ccgatggtct tggcggatta cgcgccgcgc agcagttaat
    15661 gacgcccgtg ctaaaaccgt tgttgggcat tccaggcatc tgttcactgg cgctgattgc
    15721 taacctgcaa aacaccgatg ccgccgcagg tatgaccaaa gaactggcgc aggaagggga
    15781 aattaccgaa cgtgataaag tgatattcgc cgcctatcaa accagcggta gcgccatcat
    15841 caccaactac ttctcttccg gcgtcgcggt gtttgccttt ctgggcacgt ccgtaatcgt
    15901 acctttagcc gtcatcctgg tgtttaaatt tgtcggagcc aacattttgc gcgtctggct
    15961 taacttcgaa gaacgtcgca acccaacgca aggagcgcaa gcatgacaac tcaggtccgc
    16021 aaaaacgtca tggatatgtt tattgatggc gcgcgccgtg gctttactat cgctaccacc
    16081 aacctgctgc caaacgtggt gatggccttt gtcatcatcc aggcgctgaa aattactggc
    16141 ctgctcgact gggtgggaca tatctgtgaa cccgtaatgg cgctgtgggg attgcccggt
    16201 gaagcggcaa ccgtcctttt agccgcattg atgagcatgg gcggcgcagt cggtgttgcc
    16261 gccagtctgg caaccgctgg cgcattaacc ggccacgatg tcaccgtttt actgcccgcg
    16321 atgtatttaa tgggcaaccc ggtgcaaaat gtcggtcgct gcctgggaac agccgaagtg
    16381 aatgccaaat attacccgca tatcattacg gtctgcgtga ttaacgcatt gctgtcgatc
    16441 tgggttatgc agcttattgt ttaacaagga gttaccatga ttgattattc cgcagccggt
    16501 tttaccctgc tgcagggagc gcatttgtat gcgccggaag atcggggaat ttgcgatgtc
    16561 ctcatcgcta acggcaaaat tattgccgtt gccagcaaca tcccttctga cattgtacca
    16621 gactgcacgg ttgtcgatct cagcgggcag atcctctgcc caggttttat tgatcaacac
    16681 gtccatttga ttggtggtgg cggcgaagca ggtcccacga cacgcacgcc ggaagtggcg
    16741 ttaagtcgtc tgacggaagc gggcgtcacg tcagtggttg gtctgctggg taccgactct
    16801 atctctcgcc acccggaatc cctgctcgcc aagacccgtg cgctcaatga agaaggcatc
    16861 agcgcctgga tgctgaccgg cgcttatcat gtcccttccc gcaccattac aggttccgtg
    16921 gaaaaagacg tggcgattat cgatcgcgtg attggtgtga aatgcgccat ctctgatcac
    16981 cgttctgccg cacctgacgt ttatcacctg gctaatatgg cggcggaatc ccgcgttggc
    17041 ggtttgcttg gcggtaaacc tggcgtcacc gtgttccaca tgggcgacag caaaaaggcg
    17101 ttacagcctg tctatgacct gctggaaaac tgcgatgtgc cgatcagcaa gctgctgccg
    17161 acccacgtta accgcaacgt tccgttgttt gagcaggcgc tggagttcgc gcgcaaaggc
    17221 ggcaccatcg acatcaccag tagcattgac gaaccggtcg cccctgccga aggtattgcc
    17281 cgcgccgttc aggcgggtat tccgctggca cgcgtcaccc tcagctccga cggcaacggt
    17341 agccagccgt tctttgatga cgaaggaaat ttaacccata tcggtgttgc aggttttgaa
    17401 acattgctgg aaaccgtgca aatgttggtc aaagactata gcttcagcct tagcgatgcc
    17461 ctgcgcccgc tcaccagtag cgtagccggt ttccttaacc tgaccgggaa aggcgaaatt
    17521 ctgccaggca atgacgcaga cttactggtc atgacgcctg aattgcgcat tgagcaggta
    17581 tacgctcgcg gcaaactgat ggtcaaagac ggcaaagcct gcgtgaaagg aacgtttgaa
    17641 acggcataac cggttgtcag ccgcccgaat ggacgtagaa tgcccgcaag ggcggctgac
    17701 agagtataac gtaatggatg actgtggtgc ggttttgcat aacattgaaa ccaaatggct
    17761 ttatgatttt ctgaccctgg aaaaatgtcg caatttttcc caggcggcag tcagtcgcaa
    17821 cgtctcgcaa ccggcattca gccgccgcat ccgtgcactg gaacaggcga ttggcgttga
    17881 attgtttaac cgccaggtga cgccgctgca actctcggaa caagggaaaa tcttccattc
    17941 gcagatccgc catctgttgc aacagttaga aagcaacctg gcagagctgc gtggcggcag
    18001 cgattacgcg caacgtaaaa tcaagatagc cgctgcacac tctctttccc tcggtctgtt
    18061 accgtccatt atcagccaga tgccgccgct ctttacctgg gcaattgaag ccattgatgt
    18121 cgatgaagcg gtcgataaac tgcgcgaagg gcaaagtgac tgtatttttt cctttcacga
    18181 cgaagatttg ctggaagcgc cgtttgacca cattcgctta tttgaatctc aattattccc
    18241 cgtctgcgcc agtgacgaac acggagaagc actgtttaac ctcgcgcagc cacactttcc
    18301 gttactgaat tacagccgca actcctacat ggggcgattg attaatcgca ccctgacgcg
    18361 ccacagtgag ttgagtttca gcaccttttt tgtctcttcg atgagcgagc tattaaagca
    18421 ggttgccctc gacggctgtg ggattgcctg gctgccggaa tacgctatcc aacaagaaat
    18481 tcgcagcggg cagctcgttg tactgaatcg ggaagaactg gtgatcccga ttcaggccta
    18541 cgcataccgg atgaataccc gcatgaatcc aattgccgaa cgcttctggc gtgaactgcg
    18601 cgagctggag attgaacctg gttgaaagtt ttctgcagaa aatcttccgg cgctgggaat
    18661 tatgattgag gttcctgccg cagtcatgat tgcagacaaa ttagccagtg aagtcgattt
    18721 cttttccatc ggcaccaacg atatgacgca atatattatg gcggcagaca gaggaaatag
    18781 caccgtcgcg aaactggtcg attattgtaa tgacgcggtg attaacgcta ttgctatggt
    18841 gtgtcaggcc ggacgtaata atgaaattcc ggtcagtatg tgcggcgaaa tggctggaga
    18901 tatccagcaa acagcacgac tgctcaccat ggggattgat aagctcagtg ccagtccatc
    18961 ccggcttccg gccttgaaag cagcaatcag agcctcccac taattgtgac ctaacgcgat
    19021 acgtcttgcg cgtatcgcgt ttcccgtcat ttactgcggc atcgtgcgat gccagttccc
    19081 cttctgccag accaggtcaa acgttagcgg ctcatcatcg tggtacagca tcatctccag
    19141 actaatggcc aactcgtggc aataggcttc tttcagattg acctgcaatg ccgcaatacg
    19201 tggctcatga aataaaagcg cggaacgtaa ctggcggcaa taccaactct ggagaagctt
    19261 gtcgctctga ttatgccctt gccactcagg catgcctgaa acaagtaata ttggtagcgg
    19321 tgaatgggga gaactaaaaa gttgaaagag atcccggcat acggaatcac gcagcgataa
    19381 cgtattgtgt cctgtacttt caggtaatac cgtctgaact gattggcgca tcattttgcg
    19441 cactcctgac gtttagtggc atctgtatcc tgccctggct gccggacacc actgggttga
    19501 tgctcatatt ccataatcat tcctttatgc tcattgcgtc cctggcactt tcataaggga
    19561 tttataagat ccgccaaaga taaaacactg tacagaaaag aatatccact gactttttat
    19621 ttaaaaaggt atcatttgac aaaagtcatt aaacataacg g
//
LOCUS       DAZFLD010000082        19631 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.082, whole genome shotgun sequence.
ACCESSION   DAZFLD010000082 DAZFLD010000000
VERSION     DAZFLD010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19631
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.082"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            218..718
                     /gene="tssB"
                     /locus_tag="ACHKBL_002472"
     CDS             218..718
                     /gene="tssB"
                     /locus_tag="ACHKBL_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     small subunit"
                     /protein_id="HFO5819327.1"
                     /translation="MSKKFEGSVAPRERINISYVPKTDGQTAEVELPLNMLVVGDTGN
                     TQETSPLDERQAVSVNKHNFGAVMAEAAIGLNFTVPATLKGSTTDDELNVALNIKSLD
                     DFSPDSVARQVPEVNKLLELREALTALKGPMGNLPAFRTQLQALLENEESREQLLKEI
                     GQVSGK"
     gene            753..959
                     /locus_tag="ACHKBL_002473"
     CDS             753..959
                     /locus_tag="ACHKBL_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5819328.1"
                     /translation="MAAHKNISGKHPLSDEDILQAVYRVCFSPSGEINRDGVDALITT
                     LDRRISDLLDEIIHLCEFQQSAPY"
     gene            1028..2503
                     /gene="tssC"
                     /locus_tag="ACHKBL_002474"
     CDS             1028..2503
                     /gene="tssC"
                     /locus_tag="ACHKBL_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001501045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
                     /protein_id="HFO5819329.1"
                     /translation="MSLQEEELVSSHAGQTDQASSLLDQIMAQTRIQPGSEGYDVARQ
                     GVTAFIASILQSTASAEPVNKLAVDSMIADIDERISRQMDVIIHAPAFQQVESFWRSL
                     KTMVDRVDFRENIKVNVLHVTKQELLEDFEFAPEIIQSGFYKHVYSSGFGQFGGEPIA
                     AVLGAYEFKNTAPDMKLLQYVSAVGAMAHAPFLSSVSPEFMGLNSWTELPNIKDLYAI
                     FEGPAYTKWRALRDSEDSRYLGLTAPRFLLRQPYSPTDNPVKNFNYYEDVSQNHEDYL
                     WGNTAWMLACNIADSFAKYRWCPNIIGPQSGGAVKDLPVHLFETMGQIQAKIPTEVLV
                     TDRREFELAEEGFITLTMRKDSDNAAFFSANSVQKPKHFPGKDAETNYKLGTQLPYLF
                     IINRLAHYIKVLQREQLGSWKERSDLERELNTWIRQYVADQENPPADVRSRKPLRAAK
                     VEVMDVEGEPGWYQVALSVRPHFKFMGANFELSLVGRLDRE"
     gene            2510..2923
                     /gene="tssE"
                     /locus_tag="ACHKBL_002475"
     CDS             2510..2923
                     /gene="tssE"
                     /locus_tag="ACHKBL_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HFO5819330.1"
                     /translation="MIPDRERTTGSLFERMEASSARNRQGGSIHSLRQSIRQNLRNIL
                     NTRSGSCRGAPELGIDEPEGAENFRESMSRAIEQCIERYEPRISHAEVQAVVSSASSP
                     LDMTFHITAWVTFNETHEVLEFDMAPNGSQHYRVD"
     gene            2927..4777
                     /gene="tssF"
                     /locus_tag="ACHKBL_002476"
     CDS             2927..4777
                     /gene="tssF"
                     /locus_tag="ACHKBL_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HFO5819331.1"
                     /translation="MEFEERYFREELDYLRQLSKLLATEKPHLARFLAEKDADPDIER
                     LLEGVAFLTGNLRQKIEDEFPELTHGLIKMLWPNYLRPVPAMTLIEYTPDMDKSSVPV
                     LIPRNEQFTTNAGEIRVDEVLPSDAKKEEPPPCTFTLCRDIWLLPVRLEQIENRSTTR
                     NGVINITFSVAPGTDFRTLDLNKLRFWLGNDDNYTRDQLYLWFCEYLQGADLTVGEQH
                     LRLPEFMLKAVGFEPQDAMLPWPKNVHSGYRILQEYFCYPDAFLFFDLCGCPALPDGL
                     QAEFFTLQLRFSHPLPVDIRLRRDSLRLYCAPAINLFIHHAEAITLDNRRADYPLVPS
                     RHYPEHYDVFSVNGVISQVQDMFRKKDLGRPVSTQAARQWPAFESFSHQMEYSRKREV
                     VYWHHRTKTSLFHRGFDHTLAFIHADGSYPSDESLLSNEVVSVSLTCTNRELPSQIRS
                     GDITGTTGKNAAVASFRNITRPTQPLWPVIDGSLHWSLLSAMNLNYLSLLDTDALKQV
                     IANFDRHAIHHPQTARLSQQKLDAIERLETRPVDRLFTGIPVRGLASTLYLHPEPFVC
                     EGEMYLLGTVLSHFLSLYASVNSFHMLTVVNTESQETWKWTERTGQHPLI"
     gene            4741..5823
                     /gene="tssG"
                     /locus_tag="ACHKBL_002477"
     CDS             4741..5823
                     /gene="tssG"
                     /locus_tag="ACHKBL_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HFO5819332.1"
                     /translation="MDGKNRAASSYLSPGNPPADKEQNDPLAQVFHNACSYNFFAMAE
                     LLHRLAKGEKGTPELSLRDDPAQETLRFSADASLAFPCSDISALKRDTSGAFRMTTTF
                     MGLQGSQSPLPGYYLDHLAWKAVHEQSPVGDFLDMFSHRLTQFVWHIWRKYRYHISFR
                     NGGVDAFSQRMYSLVGLGHRQLRDKLAINHSKMLAYSGILANPGRSPEIICGLVSHCF
                     DLSDVTLQNWQRRKVDIEPDQQNSLGSYSLKNGEKLAGRSVLGNFVLGTRVPDLSGKF
                     QLSITSLTRKQFLSFLPSGENFLPLTMFVSFILRDQLAWDLHLGLAPEQVGAMRLGDN
                     KSALLGWTSFLGTPEERPSVTIRVRS"
     gene            5848..7128
                     /gene="tagH"
                     /locus_tag="ACHKBL_002478"
     CDS             5848..7128
                     /gene="tagH"
                     /locus_tag="ACHKBL_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated FHA domain
                     protein TagH"
                     /protein_id="HFO5819333.1"
                     /translation="MPEEKLQTLSLQVINGSELESGRAARCVFTQQGNVGHGPECQWS
                     VQDRQQSIPAQAFTVILHDGTFCLRPQTAQLWLNQAKVTATSDLIQLRQGDEIQIGRL
                     MVRVHLNRGDIPHYDEEMATPETIVTNRDMLTDTLLSTEGAPHYPGMTHRHQLADTVV
                     NGFSTDPLQALQSESLITAGDPLSGIAAVRPSAPLSDPASNGGINTPFMDLPPIYASP
                     GDRNDDVSVAEMAQRHLAVTPLLRGLGGSLTVSNSDDADDFLEEAGRTLQAAIKGLLD
                     LQQQRNSLSDKHLRPLEDNPLRLNMDYATALDVMFAEGKSPVHLAAPAAVSESLRNVR
                     HHEEANRAAIVESLRVLLDAFSPQNLLRRFVQYRRSHELRQPLDDAGAWQMYSHYYEE
                     LASDRQQGFEMLFNEVYAQVYDRVLREKQREPEA"
     gene            7125..7649
                     /gene="tssJ"
                     /locus_tag="ACHKBL_002479"
     CDS             7125..7649
                     /gene="tssJ"
                     /locus_tag="ACHKBL_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system lipoprotein TssJ"
                     /protein_id="HFO5819334.1"
                     /translation="MNRKAFLACVLMCVILTGCETAKKISEVIKNPDIQVGSLKEQPS
                     EITVTLLTEPDTNTNAEGESAAVDVQLVYLTDDSKLLAADYDQIASTPLPDVLGKNYI
                     DHQDFNLLPDTIKTLPPVKLDEKTQFIGVVAYFSDDQATEWKQIETVEGTGHHYRLLV
                     HVRQSSIEMKKEDE"
     gene            7652..8983
                     /gene="tssK"
                     /locus_tag="ACHKBL_002480"
     CDS             7652..8983
                     /gene="tssK"
                     /locus_tag="ACHKBL_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
                     /protein_id="HFO5819335.1"
                     /translation="MATTRNKVMWQEGMLMRPHHFQQQQRYNDYLDNQRFRAMNDLSW
                     GFTELTLNNELLAQGKIMIDSASGILPDGTVFSIPDQDALPDPLHPQNFPDERSRNIY
                     LALPVASDVRNEISDGRRIGRYRLNYADVRDLHSEEGDARTLTLGQLTPRIMSGAEDM
                     SAYITLPLCRISDRHADGSLTLDDDFIPSCQNIQVSKKLRVYLKEVQGAIGGRASDLA
                     NRIGSPAQSGIADVAEFMMLQLLNRNQTRFTHRARRSQLHPEDFYLDLAGLLGELMTF
                     TEPSRLPCPLDVYDHHDLTKTFKTLLPEVKRALHTVLSPRAVNLPLHLRDGIWQADIH
                     DTELLQSATFVLAVAANMPVDQIQRQFIQQSKISSPEKIRNMVSVQIPGIPLRALMVA
                     PRQLPYHSGFSYFELDKSGQAWAEIAAAGAVALHVSGSFPDLNMQLWAIRG"
     gene            8988..9749
                     /gene="icmH"
                     /locus_tag="ACHKBL_002481"
     CDS             8988..9749
                     /gene="icmH"
                     /locus_tag="ACHKBL_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IVB secretion system protein IcmH/DotU"
                     /protein_id="HFO5819336.1"
                     /translation="MDEGSLSLPPFTGHDEKSQRDYHLALRGNSLNPMIDAATPLLGM
                     VMRLSTMNSQTMPEHLFAQVVTDVQAVEQLLQEQGYEPGVIISFRYILCTFIDEAALG
                     NGWSNKNEWIKQSLLVHFHNEAWGGEKVFILLERLIREPKRYQDLLEFLWLCFSLGFR
                     GRYKVAAQDQGEFEQIYRRLYHVLHKLRGDAPFPLLHQDKKTQGGRYQLISRLTIKHI
                     FYGGVVVLALFYLFYLLRLDSQTQDILHQLNKLLR"
     gene            9758..12541
                     /gene="tssH"
                     /locus_tag="ACHKBL_002482"
                     /gene_synonym="clpV"
     CDS             9758..12541
                     /gene="tssH"
                     /locus_tag="ACHKBL_002482"
                     /gene_synonym="clpV"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ATPase TssH"
                     /protein_id="HFO5819337.1"
                     /translation="MIQIDLPTLVKRLNLFSRQALEMAASECMSQQAAEITVSHVLIQ
                     MLAMPRSDLRVITRQGDIGMEELRQALTVENYTTARSADSYPAFSPMLVEWLKEGWLL
                     ASAEMQHSELRGGVLLLALLHSPLRYIPPAAARLLTGINRDRLQQDFVQWTQESAESV
                     VPDADGKGAGTLTDAADTLLARYAKNMTADARNGRLDPVLCRDHEINLMIDILCRRRK
                     NNPVVVGEAGVGKSALIEGLALRIVAGQVPDKLKNTDIMTLDLGALQAGASVKGEFEK
                     RFKGLMAEVISSPVPVILFIDEAHTLIGAGNQQGGLDISNLLKPALARGELKTIAATT
                     WSEYKKYFEKDAALSRRFQLVKVSEPNAAEATIILRGLSAVYEQSHGVLIDDDALQAA
                     ATLSERYLSGRQLPDKAIDVLDTACARVAINLSSPPKQISALTTLSHQQEAEIRQLER
                     ELRIGLRTDTSRMTEVLVQYDETLTALDELEAAWHQQQALVQEIIALRQQLLGVAEDD
                     AAPLPDADTVEDTPPESESESESESEQDNTGAVPADEADREQPEETAETVSPVQRLAQ
                     LTAELDALHNDRLLVSPHVDKKQIAAVIAEWTGVPLNRLSQNEMSVITDLPKWLGDTI
                     KGQDLAIASLHKHLLTARADLRRPGRPLGAFLLAGPSGVGKTETVLQLAELLYGGRQY
                     LTTINMSEFQEKHTVSRLIGSPPGYVGYGEGGVLTEAIRQKPYSVVLLDEVEKAHPDV
                     LNLFYQSFDKGEMADGEGRLIDCKNIVFFLTSNLGYQVIVEHADDPETMQEELYPVLA
                     DFFKPALLARMEVVPYLPLSKETLATIIAGKLARLDNVLRSRFGAEVVIEPEVTDEIM
                     SRVTRAENGARMLESVIDGDMLPPLSLLLLQKMAANTAIARIRLSAVDDAFTADVEDA
                     QNDESVTKDETVL"
     gene            12538..13281
                     /gene="vasI"
                     /locus_tag="ACHKBL_002483"
     CDS             12538..13281
                     /gene="vasI"
                     /locus_tag="ACHKBL_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated protein
                     VasI"
                     /protein_id="HFO5819338.1"
                     /translation="MSRLRVFRQTGWFIAGLMTGLPATAAPAETSSMAGVAVAVATTP
                     PPDATATLQAMQSCRRESAALERLDCYDRLLAPLSPSGFDGALVKAGFVGEAWTRATE
                     QEKRREGNTTELLVTQVPGERPTVVITTPAIGHVPPRPVLMFSCVDNITRMQVALMHP
                     LDVHDIAVTLNADSRALRSHWFVRENGTLLESSRGLSGIDEIKQLFGAKTLTVDTGAD
                     NAAGKLTFNIDGLARAIAPLRVACHWAGE"
     gene            13286..14698
                     /gene="tssA"
                     /locus_tag="ACHKBL_002484"
     CDS             13286..14698
                     /gene="tssA"
                     /locus_tag="ACHKBL_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssA"
                     /protein_id="HFO5819339.1"
                     /translation="MAMNLRDPNVWISHLLENLPEEKLASALKDDNPDWEYIDGEIVK
                     LGSLAHSQLDIPELQRRGLVILASESKDFRLLAHLLRTLQHAGDPHLALRLLALYVEH
                     YWSVAAPQNMAHKKRFASQVIKRFETGIEGFSQNAATAQHDALSGELAKLAQCWQSYN
                     APELALATDDLFALYQRAFNRAAPAPAPTPAASDSSRQTTATSESGVTQPSAPAPQIA
                     IDSHDDKAWRDTLLKVAAILCERQPDSPQGYRLRRHALWQSITSTPQAESDGRTPLAT
                     VSADMVADYQSRLASADMALWQQVEKSVLLAPYWLDGHCLSAQTALRLGYKQVADTIR
                     DEVIRFLERLPQLTGLLFNDRTPFLSEQTKQWLAASPDGKVAPVAQIGEESQAARACF
                     AGQGLEAALRYLDMLPEGDPRDQFHRQYLAAQLTEEAGLIQLAQQQYRMLLMIGSQMM
                     VSDWEPSLLTQLEQKFTAEQ"
     gene            14807..18241
                     /gene="tssM"
                     /locus_tag="ACHKBL_002485"
     CDS             14807..18241
                     /gene="tssM"
                     /locus_tag="ACHKBL_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system membrane subunit TssM"
                     /protein_id="HFO5819340.1"
                     /translation="MLILAWIFLLVWIWWKGPTWTLYEEQWLKPLANRWLATAAWGII
                     ALVWLTVRVMKRLQQLEKMQKQQREEAIDPLSVELNAQQRYLDRWLLRLQRHLDNRRF
                     LWQLPWYMVIGPAGSGKTTLLREGFPSDIIYAPEGARGAEQRLYLTPHVGKQAVIFDI
                     DGTLCAPADADILHRRLWEHALGWLKEKRARQPLNGIILTLDLPDLLTADKRRREHLL
                     QTLRSRLQDIRQHLHCQLPVYVVLTRLDLLQGFAALFQSLNRQDRDAILGVTFTRRAH
                     ENDDWRTELNAFWQTWVDRMNLALPDLMVAQTHTRASLFSFSRQMQGSREPLVSLLEG
                     LLDGENMNVMLRGIYLTSSLQRGQMDDIFTQSAARQYRLGNNPLASWPLVDTAPYFTR
                     GLFPQALLAEPNLATESRTWLMRSRRRLTVFSTTGGVAALLLITGWHHYYNGNYQSGI
                     TVLKQAKAFMDVPPPLGEDDYGNLQLPLLNPVRDATLAYGDWGDRSRLADMGLYQGRR
                     IGPYVEQTYLQLLEQRYLPSLFNGLVKAMNAAPPESEEKLAVLRVMRMLEDKSGRNNE
                     VVKQYMAKRWSEKFHGQRDIQAQLMSHLDYALAHTDWHAERQAGDGDAISRWTPYDKL
                     VVSAQKELSKLPVYQRVYQSLKTRALGVLPADLNLRDQVGPTFDQVFTSADDNKLVVP
                     QFITRYGLQSYFVKQRDELVELTAMDSWVLNLTRSVKYSDADRAEIQHQLTEQYISDY
                     TATWRAGMDNLNIRNFESIGQLTGALEQVISGDQPLQRALTVLRDNTQPGVFSEKLSA
                     KEREEALAEPDYQLLTRLGHEFAPENSTLAVQKDKESTMQAVYQQLTELHRYLLAIQN
                     APVPGKSALKAVQLRLDQNSSDPIFATRQMAKTLPAPLNRWVGRLTDQAWHVVMVEAV
                     HYMEVDWRDSVVKPFNEQLANNYPFNPRSAQDASLDAFERFFKPDGILDTFYQQNLKL
                     FIDNDLSLEDGDNNVIIREDIIAQLETAQKIRDIFFSKQNGLGTSFAVETVSLSGNKR
                     RSVLNLDGQLVDYSQGRNYTAHLVWPNNMREGNESKLTLIGTSGNAPRSISFSGPWAQ
                     FRLFGAGQLTGVQDGNFTVRFSVDGGAMTYRVHTDTEDNPFSGGLFSQFGLSDTLY"
     gene            18252..19631
                     /locus_tag="ACHKBL_002486"
                     /pseudo
     CDS             18252..19631
                     /locus_tag="ACHKBL_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308244.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VasL domain-containing protein"
BASE COUNT         4564 a         5429 c         5479 g         4159 t
ORIGIN      
        1 gcacaggttc acacaatcta tttaacgctt gcagaatacc cctctttacg atcgataaga
       61 agtccttttc ttatttattt tgaagtgtag aatgcggggt gatgaaggag tggtaatttt
      121 attttcaccg ccattagggc ggcattgccg atattttttt gcggtacaaa tatgccgtgt
      181 tcatggcctg tacgagataa catgacggat cacttttatg agcaaaaaat ttgaaggcag
      241 cgtcgcgcct cgcgagcgta taaatatatc ctatgtcccg aagacggacg gccagacggc
      301 tgaagtagag cttccgctta acatgctcgt tgtcggcgat accggaaata cgcaggagac
      361 atccccactg gatgaacgtc aggcagtttc cgttaacaag cacaatttcg gtgcagtaat
      421 ggctgaagca gccattggcc tcaattttac cgtccccgcc acgctgaagg gaagcacaac
      481 ggacgatgaa ctgaatgtgg cgctgaatat caagtcactg gatgattttt caccggacag
      541 cgtggcccgg caggttccgg aagtgaataa attgctggaa ctgcgtgaag cactgactgc
      601 gctgaagggg ccgatgggca accttccggc attccgtacc cagctccagg cattgctgga
      661 aaatgaggag tcccgcgagc agttgcttaa agaaatcggc caggtgagtg ggaaataacc
      721 accactattt cattaaacag ggttatccag acatggcggc acacaaaaat atctccggga
      781 aacaccccct ctctgacgaa gacattttgc aggcggttta ccgggtatgt ttcagcccgt
      841 ccggtgaaat caaccgggat ggcgtggatg cgctgattac aacactggat cgccgtatca
      901 gtgatctatt ggatgagatt atccatcttt gtgagtttca gcagagcgcg ccgtactgac
      961 ggcgcgtact tcactgacgg cgaaaacccg gtttcagaaa cacagctaaa acagaaggat
     1021 tttcagtatg tctttacagg aagaggaact cgtttccagt catgccgggc agactgatca
     1081 ggcgtcgtcg ctgcttgatc aaatcatggc acagacccgt attcagcctg gttcggaagg
     1141 ttatgacgtt gcccgccagg gtgtgacagc ctttattgcc agcatcctgc aatccactgc
     1201 atctgccgaa ccggtcaata aactggccgt ggacagcatg attgcagata tagatgaacg
     1261 catcagccgc cagatggatg tcatcatcca tgcgcctgcc tttcagcagg tcgagtcgtt
     1321 ctggcgctca ctgaaaacca tggtggatcg cgttgatttc cgtgaaaaca tcaaggtcaa
     1381 cgtgcttcat gtcacgaagc aggaacttct ggaagacttt gaatttgccc cagaaattat
     1441 tcagtccgga ttttataaac acgtttattc gtccggattc ggacagtttg gtggtgaacc
     1501 catcgctgcc gtgctgggcg cttatgagtt caaaaatacc gctcctgaca tgaaactgct
     1561 gcaatacgtc agtgccgtgg gcgcaatggc gcacgcaccg ttcctgtcct ccgtttcccc
     1621 ggagtttatg gggctgaact cgtggaccga actgcctaat atcaaagatc tttatgccat
     1681 ctttgaaggt ccggcctaca ccaaatggcg cgccctgcgt gactcggaag attcgcgcta
     1741 tttagggctt acggcacccc gcttcctgct tcgccagccc tattcaccaa cagataaccc
     1801 tgttaagaac tttaactatt acgaagatgt cagccagaac cacgaagatt atctgtgggg
     1861 taatacggcc tggatgctgg catgcaatat cgcagacagt tttgccaaat accgctggtg
     1921 tccaaacatt atcggtccgc aaagcggcgg cgcagtgaaa gatctgccgg tgcatctgtt
     1981 cgaaacgatg gggcagattc aggccaaaat cccaaccgag gtgctggtca ccgatcgccg
     2041 cgaatttgaa ctggctgaag agggttttat cacccttacc atgcgtaaag actctgataa
     2101 tgcagccttt ttctctgcaa actcggtaca aaaaccgaag cactttccgg gaaaagacgc
     2161 agaaaccaac tataagctgg gtacgcagct tccttatctc ttcatcatca atcggttagc
     2221 gcactacatc aaggtgttgc agcgtgaaca actggggtca tggaaagaaa ggagcgactt
     2281 agaaagagag cttaatacct ggatccgtca gtatgttgcc gaccaggaaa acccgcctgc
     2341 agacgtgcgc agccgcaaac ctctgcgcgc tgccaaagtt gaggttatgg atgtagaagg
     2401 cgaacccggc tggtaccagg ttgcgctaag cgtgaggcct cattttaaat ttatgggggc
     2461 aaattttgag ctttccctgg ttggccggtt agacagggag taacacacca tgataccgga
     2521 tcgggaaaga accacaggtt ctctttttga gcgtatggaa gcctcttcag ccagaaaccg
     2581 gcagggaggc agtattcatt cgctcaggca atccattcgc cagaacctac gaaacattct
     2641 taatacccgg tccggtagtt gccggggtgc gcctgagctg ggcattgatg aaccggaagg
     2701 ggctgaaaac ttccgcgaat caatgagtcg ggccatcgag caatgtatag aacgctatga
     2761 accccggatc tcacacgctg aagtgcaggc agtggtttca tccgcctcca gcccgctgga
     2821 tatgacgttt catattacag cctgggtgac atttaacgaa acgcatgagg tgcttgaatt
     2881 tgatatggca ccgaatggca gccagcatta ccgggtggac tgacaaatgg aatttgaaga
     2941 acgctatttc cgggaagaac tcgattacct gcgccagctt agcaagctgc tggcaacgga
     3001 aaaaccccat ctggcccgct tcctggccga aaaagatgca gatccggata ttgaacgcct
     3061 gctggaaggg gtggcttttc ttaccggcaa tctccgccag aaaattgagg atgaattccc
     3121 agaactgacg cacgggctta ttaagatgct ctggcctaat tacctgcgtc cggttccggc
     3181 aatgaccctt attgaatata cgccggatat ggataagtct tctgtaccgg tgttaatccc
     3241 ccgtaatgag cagtttacaa ccaacgccgg ggaaatcaga gttgatgaag tgctgccctc
     3301 tgatgctaaa aaggaggagc ctcctccctg taccttcacc ctctgccggg atatctggct
     3361 gctgcccgtt cgcctggagc agattgaaaa ccgcagtacg acccgtaatg gtgttatcaa
     3421 catcaccttt tcggtcgcac cgggaacgga cttccgcacg ctggatctga acaaacttcg
     3481 cttctggctc ggcaatgacg acaactatac ccgtgaccag ctttatttat ggttctgcga
     3541 atacttgcag ggtgctgacc tgactgtggg tgaacagcat cttcgcctgc ctgagtttat
     3601 gctaaaagct gtcggttttg agccgcagga tgccatgctg ccctggccga aaaacgtcca
     3661 cagcggctac cggatccttc aggagtattt ctgttacccc gatgcgtttc tcttttttga
     3721 tctttgtggt tgtccggctt tgcctgacgg attgcaggcg gaattcttta ccctgcaact
     3781 gcgtttttcg cacccattgc ccgtggacat ccggttgcgc cgcgactccc tgcgcctgta
     3841 ttgcgcgcct gccattaatt tatttatcca ccatgcagaa gccatcacgc tggacaaccg
     3901 gcgggcagac tatccgctgg ttcccagccg ccattatccg gaacattacg atgtcttttc
     3961 cgttaacggc gtgataagcc aggtgcagga tatgttcagg aaaaaagatc tgggtcgtcc
     4021 tgtttcgacg caggccgcgc gccagtggcc tgcctttgaa agtttcagcc atcagatgga
     4081 atacagccgg aagcgggaag tggtgtactg gcatcaccgg accaaaacat ccctgttcca
     4141 tcgcggcttt gatcataccc ttgcttttat acatgctgat ggcagttatc cgtcagacga
     4201 atcgctgctc agtaatgaag tggtttcggt atcgctgacc tgtaccaacc gtgaacttcc
     4261 gtcacaaatt cgttccggcg atatcaccgg cacaaccggt aaaaatgcgg ctgttgcttc
     4321 atttcgcaac attacccgcc cgacgcaacc gctctggccg gtcattgatg gcagcctgca
     4381 ctggtccctg ctctccgcca tgaacctgaa ttatctgtca ttactggata cggacgcgct
     4441 gaagcaggtc atcgccaact ttgatcgcca cgcaatccat catccgcaga cggcgcggct
     4501 gtcacaacaa aagctggatg ccattgagcg tctggagacc cgccctgtcg atcgcctgtt
     4561 tacgggcatt cccgtccggg ggctggcctc cacgctgtat ctgcacccgg agccgtttgt
     4621 ctgtgaaggg gaaatgtatc tgctcggtac ggtgctttcg cattttctgt cgctgtacgc
     4681 cagcgttaac tcattccaca tgctgaccgt tgtgaacaca gaaagccagg agacatggaa
     4741 atggacggaa agaaccgggc agcatcctct tatctgagtc cgggaaatcc cccggcagat
     4801 aaagagcaga atgatccgct ggcacaggtt tttcataacg cctgctctta caattttttt
     4861 gcgatggcgg agctgttgca ccgcctggca aagggtgaaa aggggacgcc agaattatcc
     4921 ctgcgtgacg atccggcaca ggaaaccctg cgtttcagcg ccgatgccag ccttgccttc
     4981 ccgtgcagtg atatcagtgc gctgaaacgg gatacgtcag gggcattcag gatgaccacc
     5041 acctttatgg ggttacaggg gagccagtcg cctctccccg gttattacct cgatcacctg
     5101 gcctggaaag cggtacatga acaaagtccg gtaggtgatt ttctggatat gttcagccac
     5161 cgcctgaccc agtttgtctg gcatatctgg cgtaagtacc gctatcacat cagttttcgt
     5221 aacggcggtg tggatgcgtt ctcgcagcgc atgtactccc ttgtcgggct gggccaccgc
     5281 cagcttcgcg ataagctggc gattaatcac agcaaaatgc tggcgtattc cgggatcctg
     5341 gcaaatccgg gacgatcccc ggaaataatc tgcggcctgg tgtcccactg ctttgatttg
     5401 tcagacgtga cgctgcaaaa ctggcaacgt cgcaaggtgg atattgaacc ggatcagcaa
     5461 aacagtctgg ggagctattc acttaaaaat ggcgaaaaac tggcgggaag atcggtactg
     5521 ggcaactttg ttctgggtac acgggtgccc gatctcagcg gaaaattcca gctcagtatt
     5581 accagtctga cccgaaaaca gttcctctct tttctgccgt ccggcgaaaa cttcctgcca
     5641 ctgacgatgt ttgtgtcctt catcctgcgc gatcagcttg cctgggactt acatctgggg
     5701 ctggcgccgg agcaggtggg cgcaatgcgc ctgggcgata acaaaagtgc gctgctgggc
     5761 tggaccagct tcctcggcac accggaagaa cgaccctcag tcaccatcag ggtacggtcg
     5821 taaattatga taatcacgga gcagaatatg ccggaagaga aactacagac gctttcattg
     5881 caggtcatta acggcagtga gctggaaagc gggcgggcgg cgcgctgtgt gttcacacag
     5941 cagggaaatg tcggccatgg ccccgaatgc caatggtcgg tacaggatcg tcagcagagc
     6001 attccggctc aggcttttac cgttatcctg cacgatggca cattttgtct gcgcccgcag
     6061 acggcacaac tgtggctgaa ccaggcaaaa gtcacagcaa catcagacct gatacagctg
     6121 cgccagggcg atgagatcca gatagggcgt ctgatggtga gggttcatct gaaccgggga
     6181 gatatccccc attacgatga ggaaatggcc actcccgaaa ccatcgtcac caatcgcgat
     6241 atgctcacgg ataccctgct gtcaacggag ggtgcgccac actatccggg aatgactcac
     6301 cggcatcagc ttgcagacac cgtggtaaac ggtttttcta ccgatccact ccaggcactt
     6361 cagtccgaaa gcctgattac cgcgggcgat ccgctttcag gcattgcggc tgtccggcca
     6421 tcggcaccgc tgtccgatcc ggcaagtaat ggggggatca atactccgtt tatggatctg
     6481 ccgcccattt atgccagccc cggcgatcgt aatgatgacg tctctgtggc agaaatggcg
     6541 caacgccacc ttgcggtcac ccccttactg cgcggtctcg gcggttcgct taccgtgagc
     6601 aattccgacg atgcggatga ttttctggaa gaggccggac gcacgttaca ggccgcaata
     6661 aaaggtctgc tcgatttgca gcaacagcgt aacagcctct cagacaaaca tttgcgcccg
     6721 ctggaagata acccgctgcg cctgaacatg gattacgcca ccgcgctcga cgtgatgttt
     6781 gccgaaggta aaagtccggt acatctggcg gctcccgccg ctgtcagtga aagcctgcgc
     6841 aatgtccgcc accacgaaga agctaaccgg gcagcgattg tggagtcact tcgtgtcctg
     6901 cttgacgctt tctcaccaca aaatctgctg cgccgctttg tgcagtaccg ccgcagccat
     6961 gaactgcgcc agccgctgga tgatgccgga gcatggcaaa tgtacagcca ttattacgaa
     7021 gaactggcct ccgatcgcca gcaggggttt gagatgctgt ttaacgaggt ctacgcccag
     7081 gtctatgacc gggtgcttcg tgaaaaacag cgggagccgg aagcatgaac aggaaggcgt
     7141 ttctggcctg cgtgctgatg tgcgtcatat taaccggctg cgaaacggcg aaaaaaatca
     7201 gcgaggtgat caaaaacccg gatatccagg tcgggagcct gaaggagcaa ccttcggaaa
     7261 taaccgtcac cctgctgacc gaaccggaca ccaataccaa tgcggagggt gaatcagccg
     7321 ccgtggatgt gcaactggtg tacctgactg acgactcaaa attgctggca gccgactatg
     7381 accagatagc cagtacgccg ctaccggatg tgttgggtaa aaactatatc gaccaccagg
     7441 atttcaacct gttgccggac accatcaaaa ccctgccgcc ggtaaagctg gatgagaaaa
     7501 cgcagtttat tggtgtcgtg gcctatttct ccgatgacca ggccactgaa tggaaacaaa
     7561 tcgagacggt ggaaggaacc ggccaccatt accgcctgct ggtgcatgta cgtcaaagca
     7621 gcatcgaaat gaaaaaagag gatgaataat tatggcaacc acccgcaaca aggtgatgtg
     7681 gcaggaaggg atgctgatgc gcccacacca tttccagcaa cagcagcgtt acaacgacta
     7741 cctggataac cagcgtttcc gggccatgaa tgatttatcc tggggattta ccgaactcac
     7801 cctcaacaat gaactgctgg cgcagggtaa gatcatgatt gacagcgcgt caggcatact
     7861 gcccgacggc accgtctttt ctatccccga ccaggacgca ctgcccgatc cgctgcaccc
     7921 gcaaaatttt ccggacgaaa gaagccgcaa tatttacctt gctctgccgg tcgccagtga
     7981 tgtgagaaat gaaatcagcg acgggcggcg aatcgggcgt taccggctga attatgccga
     8041 tgtccgggat ctgcattcag aagaaggtga cgcgcgaaca ctgacgctgg gtcaactgac
     8101 gccgcgcatt atgagcggtg cagaagatat gagcgcctat attacgctcc cgctttgccg
     8161 tatcagcgat cgccatgccg acggctccct gacgctggat gacgatttta tcccctcctg
     8221 ccagaatatt caggtcagta agaaactgcg tgtttatctc aaagaggtac aaggggccat
     8281 tggcggacgg gcaagcgatc tggcaaaccg cattggctct ccggcgcaga gcggcatcgc
     8341 ggatgtggcg gaatttatga tgttgcagtt acttaaccgt aaccagaccc ggtttaccca
     8401 tcgcgctcgt cgatcccagc tccacccgga agatttctac cttgatcttg ccgggttgct
     8461 gggtgaactg atgaccttta ccgagccgtc gcgcctgccc tgcccgcttg atgtgtatga
     8521 tcatcatgac ctgaccaaaa catttaaaac gctgttaccg gaagtcaaac gggcgctgca
     8581 taccgtactg tcgccaagag ccgtcaatct gccgctgcat ctgcgtgacg gtatctggca
     8641 ggccgatatt catgacacag aactgctgca atctgccacc tttgtgctgg ccgtggcggc
     8701 aaacatgccg gtcgatcaga tccagcgcca gtttatccag cagtcgaaaa tttcctcgcc
     8761 ggaaaaaatc cgcaatatgg tcagtgtgca gatacccggc attccattgc gtgccctgat
     8821 ggtggctccc cgccagcttc cttaccattc cgggttcagc tatttcgaac tcgacaaaag
     8881 cggccaggcc tgggctgaaa tagctgccgc cggggccgtt gcactgcatg tttccggcag
     8941 tttcccggat ctgaacatgc aactgtgggc gataagaggg taagacgatg gatgaggggt
     9001 cactgtctct gccaccattt accggacacg acgaaaaaag ccagcgcgac taccatctcg
     9061 cgcttcgtgg caacagcctg aatccgatga tcgacgcggc cacgccgcta ctcggtatgg
     9121 taatgcgcct ttcaaccatg aacagccaga cgatgccgga gcatcttttt gctcaggtgg
     9181 tgacggatgt tcaggccgtg gagcaactgc ttcaggagca gggctacgaa ccgggcgtga
     9241 ttatctcgtt tcgctacatc ctctgcacgt ttattgatga agcggcgctc ggtaacggct
     9301 ggtcgaacaa gaacgagtgg atcaaacagt cattgctggt ccatttccac aacgaagcct
     9361 ggggtggaga gaaagttttc atcctgctgg agcgcctgat ccgtgaaccc aaacgttacc
     9421 aggatctgct ggaatttctc tggctctgtt tttctctggg atttcgcggg cgctacaaag
     9481 tggcagcaca ggatcagggg gaatttgagc agatctaccg ccgcctgtac cacgtcctgc
     9541 ataaactacg cggcgatgcc cctttcccgc tgttacacca ggacaaaaaa acccagggcg
     9601 ggcgttatca gctcatcagt cgcctgacca ttaaacatat tttttatggt ggcgttgtcg
     9661 tgctggcgtt gttttacctg ttctatctgt tgcggctgga cagccagacg caggacatcc
     9721 tgcaccagct aaacaaatta cttcgataag gacgtttatg atccagattg atcttcccac
     9781 gctggtaaaa cggcttaacc tgttctcccg ccaggcgctg gagatggccg cctctgaatg
     9841 tatgagccag caggcagcgg aaattaccgt cagccatgtg cttattcaga tgctcgccat
     9901 gccacgcagt gacctgcggg ttattacccg ccagggcgat attggcatgg aagagttgcg
     9961 ccaggcgctg acggtggaga actacacaac tgcccgttct gcggacagct acccggcgtt
    10021 ttccccgatg ctggttgagt ggctgaaaga gggctggctg ctggcgtcgg ctgagatgca
    10081 gcacagcgaa ttgcgtggcg gcgtgttact gctggccctg ctgcattcgc cgctgcgtta
    10141 tataccgcct gctgccgccc ggctgttgac cggcattaac cgtgaccgtc tgcaacagga
    10201 ctttgtacag tggacacagg agtcggcgga atcagtcgtg ccggatgcag acggtaaagg
    10261 cgcaggcaca ctgacggacg ctgctgacac cctgcttgcc cgctacgcca aaaacatgac
    10321 cgcagacgcc cgtaacggca ggcttgaccc ggtactgtgc cgcgaccacg aaatcaacct
    10381 gatgatcgac attctctgcc gccgccgcaa gaacaacccg gtggtggtgg gcgaagcagg
    10441 cgtgggtaaa agtgccttaa tcgaagggct ggcgctgcgc atcgtggcag gccaggtgcc
    10501 ggacaaactg aaaaacaccg atatcatgac ccttgacctg ggcgcattgc aggccggggc
    10561 gtcggtgaag ggcgaattcg aaaaacgctt caaggggctg atggcggagg tcatttcctc
    10621 tccggtgccg gtcattctgt ttattgacga agcacatacc ctgattggcg cgggcaacca
    10681 gcagggcggg ctggatatct ccaacctgct caaaccggcg ctggcgcgcg gcgaactgaa
    10741 aaccatcgcc gccaccacct ggagcgaata caaaaaatac ttcgagaaag atgccgctct
    10801 gtcgcgccgc ttccagttag tgaaggtcag cgaacccaac gccgctgaag ccaccattat
    10861 tctgcgtggt ctgtcggcgg tctatgaaca gtctcacggc gtgctgattg atgatgacgc
    10921 cttgcaggcc gcagcaacgc tgagcgaacg ttatctctcc gggcgtcagt tgccggacaa
    10981 agccattgat gtgctggata ccgcctgcgc ccgcgtcgcc attaaccttt cctcgccgcc
    11041 gaagcaaatc tcggcgctga ccactctgag ccaccagcag gaggcggaaa ttcgccagct
    11101 tgagcgcgag cttcgcatcg ggctgcgtac cgacacatca cggatgaccg aggtgctggt
    11161 gcagtatgat gaaacgctga cggcgctgga tgaactggag gccgcctggc atcagcagca
    11221 ggcgctggta caggagatta tcgcgctgcg ccagcaattg ctgggcgtgg cagaggacga
    11281 tgcggcgccg ttgccggacg cagataccgt ggaggatacg ccgccagagt cagagtcaga
    11341 gtcagagtca gagtcagaac aggataatac cggtgccgta ccggctgatg aggccgacag
    11401 agaacagccg gaagagaccg ctgaaacagt ttccccggta cagcggctgg cccagctcac
    11461 tgccgaactg gacgccctgc ataacgaccg gttgctggtc tccccgcacg tcgataaaaa
    11521 acagattgcg gcggtgattg ccgaatggac cggcgtgccg ctcaaccgcc tgtcgcagaa
    11581 tgaaatgtcg gtcatcaccg acctgccgaa atggctgggc gacaccatca aaggccagga
    11641 cctggcgatt gccagcctgc ataagcatct actgaccgca cgcgcagacc tgcgtcgtcc
    11701 gggacgcccg ctcggtgcgt tcctgctggc tggccccagc ggtgtgggta aaaccgaaac
    11761 cgtcctgcaa ctggcagagc tgctctacgg cggtcgccag tacctgacca ccatcaatat
    11821 gtccgaattc caggagaaac acaccgtctc gcggctgatt ggttcgcctc cgggctacgt
    11881 tggctacggt gaaggcggcg tactgaccga agcgattcgc cagaaaccct actcggtagt
    11941 actgctcgat gaagtggaaa aagcgcaccc ggatgtgctc aacctgttct accagtcgtt
    12001 cgataagggc gaaatggcag acggtgaagg ccgcctgatt gactgcaaaa atatcgtctt
    12061 cttcctgacg tccaacctcg gctaccaggt aatagtcgag cacgccgatg acccggaaac
    12121 catgcaggaa gaactgtatc cggtgctggc cgacttcttc aaacctgccc tgctggcgcg
    12181 tatggaagtg gtgccgtatc tgccgctgtc gaaagagacg ctcgccacca ttatcgccgg
    12241 aaaactggcc cgtctggata acgtgctgcg cagtcgcttt ggtgcagaag tggtcattga
    12301 accggaagtg acggacgaaa tcatgagccg cgtcacccgc gcggaaaacg gcgcgaggat
    12361 gctggaatcg gtcatcgatg gcgacatgct accgccgctc tcgctgctgc tgttgcagaa
    12421 aatggcggct aacacggcga ttgcccggat tcggttgtcg gcggtggacg acgcatttac
    12481 ggcagacgtg gaagatgctc agaacgacga gtccgtcaca aaggatgaaa cggttttatg
    12541 agcaggttgc gcgttttccg gcagacagga tggtttatcg cgggcctgat gacgggctta
    12601 cccgccactg ccgcgcccgc agaaacatcg tcaatggccg gtgtggcggt cgccgtcgcc
    12661 accacgcccc cgccggatgc gacagccacg ttacaggcca tgcagtcctg tcggcgggag
    12721 tccgcggcgc tggagcggct tgactgctat gaccgcctgc tggcaccgct gtccccgtca
    12781 gggtttgacg gggcgctggt caaagccggt tttgtgggcg aggcctggac ccgcgccaca
    12841 gagcaggaaa aacgccgcga gggcaacacc acagaactgc tggtgacgca ggtgccgggg
    12901 gagcgcccga cggtggtgat aaccaccccg gctatcggcc atgttccgcc gcgcccggtg
    12961 ctgatgttca gttgcgtgga caatatcacc cggatgcagg tggcgctgat gcaccctctg
    13021 gatgtccatg atatcgccgt cactctgaat gccgacagcc gggcattgcg tagccactgg
    13081 tttgtgcgcg agaacggcac cctgctggag tccagccggg ggctgtccgg gattgatgaa
    13141 attaagcaac tgttcggcgc aaaaacgctg acagtggata ccggcgcaga taacgccgca
    13201 ggaaagctga cctttaacat tgacgggctg gcaagggcga ttgcccccct gcgcgttgcc
    13261 tgccactggg cgggagaata aaaatatggc aatgaactta cgcgatccga atgtctggat
    13321 atcgcacctg ctggaaaacc tgccggaaga aaaactggca tcggcgctga aagatgacaa
    13381 cccggactgg gagtatatcg acggcgaaat cgtcaaactg ggatctctgg cccacagtca
    13441 actggatatc ccggaactcc agcgccgggg gctggtcatt ctggcttcgg agagtaagga
    13501 ctttcgtctg ctggcgcatc tgctgagaac cctgcaacac gccggtgatc ctcatctcgc
    13561 attgcgcctg ctggcgctgt atgtggagca ttactggtcg gtggctgcgc cgcagaatat
    13621 ggcacataaa aaacgctttg ccagccaggt cattaaacgc tttgaaacag gtattgaagg
    13681 cttttcacag aacgccgcca cagcgcagca cgacgcgctg tcaggggaac tggcgaaact
    13741 ggcgcagtgc tggcagtcat ataacgcccc ggaactggca ctggctaccg atgatctttt
    13801 tgccctgtac cagcgtgcgt ttaatcgtgc ggcaccggct cctgccccca ctccggcggc
    13861 ctccgatagt tcacgacaga ccaccgccac gtctgaaagc ggcgtgacgc aacccagtgc
    13921 tccggctccc caaatcgcca tcgacagtca cgacgacaaa gcctggcgcg acacgctgtt
    13981 aaaagtggcg gctattttat gtgaacgcca gccggactcg ccgcagggct atcgcctgcg
    14041 ccgccatgcc ctgtggcaat ccatcaccag tacaccccag gcggaaagcg atggacgtac
    14101 cccattggct acggtctctg ccgatatggt ggcggattac cagtcccggc ttgccagcgc
    14161 ggatatggcg ctgtggcagc aggttgagaa aagcgtattg ctggctcctt actggctgga
    14221 cggtcactgt ctttctgcac agacggcact gcgtctgggt tacaaacagg tggcagacac
    14281 catccgcgat gaggtcatcc gcttccttga gcgtctgccc cagcttaccg ggctgctgtt
    14341 taatgaccgc acaccgtttc tcagtgagca gacgaaacaa tggctggcag cttcgcccga
    14401 cggcaaagtt gcaccggttg cgcaaatcgg tgaggaatcg caggcagcca gagcctgttt
    14461 tgctgggcag ggtctggagg cggcgctgcg atatctggac atgctacccg aaggcgatcc
    14521 ccgcgatcag tttcaccgcc agtaccttgc cgcacagttg acggaggagg cggggctgat
    14581 acagcttgcg cagcaacagt accggatgtt gttgatgata gggagtcaga tgatggtgtc
    14641 tgactgggag ccatcattac ttacgcagct tgaacaaaaa ttcacggcag aacaataaaa
    14701 gagaacagga gtttttgtgt tcagattacc aacaccccga ctactcagcg gactcaaatc
    14761 agccctacgc ccggcgatgc cccggtttaa agtctccgct ttctggctgc tgatactggc
    14821 gtggattttt ctgctggtat ggatctggtg gaaaggcccg acatggacgc tgtatgaaga
    14881 acagtggctc aaaccactgg cgaaccgctg gctggcaacg gcggcatggg ggattatcgc
    14941 cctagtgtgg cttaccgtcc gggtgatgaa gcgcctgcaa cagcttgaaa aaatgcaaaa
    15001 gcagcagcgc gaggaggcca ttgatccgct cagtgtggag ctgaacgccc agcagcgtta
    15061 ccttgaccgc tggctgctgc gcctgcaacg ccatctcgac aaccgccgtt tcctgtggca
    15121 gttgccgtgg tatatggtca tcggcccggc gggcagtggc aaaacgacac tgctgcgcga
    15181 gggttttccg tccgacatta tttatgcccc ggagggcgca cgcggcgcag aacaacgcct
    15241 gtacctcacg ccccatgtcg gtaaacaggc ggtgatcttt gatatcgacg gcacactctg
    15301 cgctcccgct gatgcggata tcctgcatcg ccgtctgtgg gaacatgccc tcggctggct
    15361 gaaagaaaag cgcgcgcgcc agccgctgaa cgggattatt ctgacactgg atttacccga
    15421 tctgcttacc gcagacaaac gccgccgcga gcatctgtta cagacgctgc gcagccgctt
    15481 gcaggatata cgccagcatc ttcactgcca gttaccggtt tacgtggtac tcacccggct
    15541 tgatttattg cagggttttg ccgccctgtt ccagtcactg aacagacagg atcgcgatgc
    15601 aattctaggg gtcacgttca cccgccgcgc ccatgaaaat gatgactggc gaacagagtt
    15661 gaatgctttc tggcagacat gggtggatcg aatgaaccta gcgttgccgg atctgatggt
    15721 cgctcagact cacacccgcg cgtctttatt tagtttttcc cgccagatgc agggaagccg
    15781 tgaaccgctg gtgtcactgc ttgagggtct gcttgacggc gaaaatatga acgtgatgct
    15841 gcgtggtatc tatctcacct cttcgcttca acgcggacag atggatgata ttttcaccca
    15901 gtctgccgcc cgccagtacc ggctgggcaa taacccactg gcgtcctggc ccctggtgga
    15961 caccgcgcct tatttcaccc gtggcctgtt cccgcaggca ttactcgcag agcctaatct
    16021 ggcaacagaa agccgcacct ggctgatgcg ttcccgtcgc cgcctaacgg ttttctctac
    16081 cacaggaggc gtggcggcac tgctgctcat caccggctgg catcactatt acaacggtaa
    16141 ctatcagtcc ggcatcaccg tgcttaagca ggcaaaagcc tttatggatg taccgcctcc
    16201 gctgggggaa gatgattacg gcaacctgca actgccgctg ctgaacccgg tacgcgatgc
    16261 cacgctggcc tatggcgact ggggcgaccg cagccgtctg gccgatatgg gactgtacca
    16321 gggacggcgt atcgggcctt atgtggaaca aacctatctg caactgctgg agcagcgtta
    16381 cctgccttcg ctgtttaacg ggctggtcaa agcgatgaac gccgcgccgc cggagagtga
    16441 ggaaaaactc gccgtgctgc gtgtgatgcg aatgctggag gacaaaagcg gacgcaataa
    16501 tgaggtggtg aagcagtata tggcaaaacg ctggagcgaa aaattccacg gccagcgcga
    16561 tatccaggca caactgatgt cccatcttga ctacgcgctg gctcatactg actggcatgc
    16621 agagcgtcag gcgggcgacg gtgacgccat cagccgctgg acgccatatg acaagctcgt
    16681 ggtatcagca cagaaagaac tgagcaaact gcctgtctac cagcgggttt atcagagcct
    16741 gaaaacgcgg gcgctgggcg ttcttcctgc tgacctcaat ctgcgtgacc aggtagggcc
    16801 aacctttgac caggtgttta catctgccga tgacaacaaa ctggttgttc cacagtttat
    16861 cacccgctat ggcctgcaaa gctattttgt aaaacagcgc gatgaactgg ttgaactgac
    16921 agcgatggat tcctgggtac ttaacctcac ccgcagtgtg aaatacagtg acgccgaccg
    16981 tgcggaaatc cagcaccagt tgaccgagca gtatatcagc gactacaccg ccacctggcg
    17041 ggccgggatg gacaatctga atatccgcaa ttttgagtcc atcggacaac tgaccggggc
    17101 gctggagcag gttatcagcg gcgaccagcc tttgcagcgg gcgctgaccg tgctgcgtga
    17161 caacacacag ccaggcgtct tttctgaaaa actctctgcc aaagaacggg aggaagccct
    17221 ggcagagccg gattaccagt tactcacccg cctcgggcat gaattcgccc cggaaaacag
    17281 taccctggca gtacagaaag acaaagaaag cacgatgcag gccgtgtatc agcaactcac
    17341 cgagttgcac cgctacctgc tggcaatcca gaacgcgcct gtaccaggga aatcggcgct
    17401 gaaagccgtg cagttacggc ttgatcagaa cagcagcgat ccgatattcg ccacccgcca
    17461 gatggcaaaa acgctgcctg ctccgctcaa ccgctgggtt ggcagactga ctgaccaggc
    17521 ctggcatgtg gtgatggtgg aggctgttca ttatatggaa gtggactggc gcgacagcgt
    17581 ggtgaaaccg tttaacgagc aactggcaaa taactatccg tttaatccgc gttctgcaca
    17641 ggatgcctca ctggatgcct tcgaacgctt ctttaaaccg gatggcatac tggatacctt
    17701 ctaccagcag aacctgaagc tgtttatcga taatgacctg agtctggagg atggcgataa
    17761 caacgtcatt attcgcgaag atattattgc gcaactggaa actgcgcaga aaatccgtga
    17821 catcttcttc agcaaacaga acggtctggg aacatccttt gccgtggaaa cggtatcgct
    17881 ttccggcaat aaacgccgca gtgtactgaa ccttgacggt cagttagtcg attacagcca
    17941 gggccgtaac tataccgccc atctggtctg gcctaacaac atgcgcgaag gcaacgaaag
    18001 taagctgacg ctcatcggca ccagcggcaa cgcgccgcgc agtatcagct tcagcgggcc
    18061 gtgggcgcag ttccgcctgt tcggggccgg acaactgacc ggagtacagg atggcaactt
    18121 taccgtgcgc tttagcgtgg acggtggcgc gatgacctac cgtgtgcata ccgacaccga
    18181 ggataacccg ttcagcggtg ggttgttcag ccagtttggt ctgtcagaca cactgtactg
    18241 aggaggcagg gatgaatagt aacgtactga cacaaactat cgttaccggc agtgacccgc
    18301 gcgggctgcc ggaattcagc gccattcgcg aggaaataaa caaagccagc cacccgtcac
    18361 agcctgagct gaactggaaa ctggtggagt cgctggcgct ggcgattttt aaagctaacg
    18421 gtgtggattt acacaccgcc acctactata cgcttgcacg gacacggaca cagggactgg
    18481 cgggcttttg cgaaggtgcg gaactgctgg cggcaatggt aagccacgac tgggataagt
    18541 tctggccaca gggcggtccg gcgcgtactg aaatgctgga ctggtttaac tcccgaaccg
    18601 gcaatattct gcgtcagcaa atctcctttg cggaatccga cctaccactg atctaccgca
    18661 cagagcgggc attgcagctt atctgcgaca agctccagca ggtagagctg aagcgcgttc
    18721 cgcgcgtgga gaatctactc tattttatgc agaacacgcg taaacggctt gaaccccagc
    18781 cgaagagtaa cgcagagaac gccgcacaga ccacggtcag aacgctgatt tatgccccgg
    18841 aaacaatggc ttcctccacg ccagaagcgg tagtgcctcc cctgcccgac ctgcctgaga
    18901 tgaaagtgga agtgcgcagt ctgtcagaaa atcccccaca ggccagtgtg atgaagcaag
    18961 gcagtacggt aagagggttt atcgcaggga tcgtctgttc agtggctatc gcctcagcat
    19021 tgtggtggtg gcaggtctat ccggcgcagc agcaactgtt acaggttaac gacaccgcgc
    19081 agggcgcagc aacggtgtgg atggcctcac ctgaacttga aaactatgag cgcaggctgc
    19141 atcagcttct tggcgtctcc ccggtacaac cgctggaaac cgggatgcag atgatgcgtg
    19201 ttgccgacag tcgctggccg gaaagcctgc aacagcagca ggcctcgaca caatggaatg
    19261 aggcactcaa aacccgcgca cagagtagcc cacagttgcg cggctggttg cagacccgcc
    19321 aggacttaca tgcttttgct gatctcgtga tgcagcgcga gaaagaggga ttaacccttt
    19381 cctatatcaa aaatgtcatc tggcaggcgg agcggggact ggggcaggaa acacctctgg
    19441 aatccctgct gacgcagtac caggatgccc gtgcgcagaa gcagaataca gatgcgctgg
    19501 aaaaacagat taacgagcgg atggacggcg tgttaagtcg ctggctgctg ctgaagaata
    19561 atacgatacc gacgataaaa aaagacattg aatttcaata acattcatga atacaaagga
    19621 gttttaaatg g
//
LOCUS       DAZFLD010000083        19471 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.083, whole genome shotgun sequence.
ACCESSION   DAZFLD010000083 DAZFLD010000000
VERSION     DAZFLD010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19471
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.083"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            87..629
                     /gene="yfaZ"
                     /locus_tag="ACHKBL_002487"
     CDS             87..629
                     /gene="yfaZ"
                     /locus_tag="ACHKBL_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaZ family outer membrane protein"
                     /protein_id="HFO5819341.1"
                     /translation="MKKIALAGLAGMLLVSASVNAMSISGQAGKEYTNIGVGFGTEST
                     GLALSGNWTHNDDDGDVAGVGLGLNLPLGPLMATVGGKGVYTNPNYGDEGYAAAVGGG
                     LQWKIGNSFRLFGEYYYSPDSLSSGIQSYEEANAGARYTIMRPVSIEAGYRYLNLSGK
                     DGNRDNAVADGPYVGVNASF"
     gene            729..1931
                     /gene="yfaY"
                     /locus_tag="ACHKBL_002488"
     CDS             729..1931
                     /gene="yfaY"
                     /locus_tag="ACHKBL_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide deamidase-related
                     protein YfaY"
                     /protein_id="HFO5819342.1"
                     /translation="MLKVEMLSTGDEVLHGQIVDTNAAWLADFFFHQGLPLTRRNTVG
                     DNLDDLVTILRERSQHADVLIVNGGLGPTSDDLSALAAATAKGEGLVLHEAWLKEMER
                     YFHERGRVMAPSNRKQAELPASAEFINNPVGTACGFAVQLNRCLMFFTPGVPSEFKVM
                     VEHEILPRLRERFSLPQPPVCLRLTTFGRSESDLAQSLDTLQLPPGVTMGYRSSMPII
                     ELKLTGPASEKQAMEKLWLDVKRVAGQSVIFEGTEGLPSQISRELQTRQFSLTLSEQF
                     TGGLLALQLSRAGAPLLACEVVPSQEETLAQTAHWITERRANHFAGLALAVSGFENEH
                     LNFALATPDGTFALRVRFSTTRYSLAIRQEVCAMMALNMLRRWLNGQDIASEHGWIEV
                     VESMTLSV"
     gene            2151..2933
                     /gene="yfaX"
                     /locus_tag="ACHKBL_002489"
     CDS             2151..2933
                     /gene="yfaX"
                     /locus_tag="ACHKBL_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HFO5819343.1"
                     /translation="MLESSKVPALTRAIDILNLIARIGPCSAATIIDTLGIPKSTAYL
                     LLNELRRQRFLSLDHQENFCLWTRLVELSGHALSKMDLRELARPRLTQLMDTTGLLCH
                     LGIIDNGSAYYILKVESSATISVRSHEGKSLSLYRSGIGKCLLAWQPAAVQQSIIEGL
                     IWEQATPTTITHPQQLHEELARIRRQGWSYDNGEDYADVRCVAAPVFNANNELTAAIS
                     VVGTRLQINEEYRDYLAGKAIACARDISRLLGWKSPFDLQAS"
     gene            2948..4153
                     /gene="rhmD"
                     /locus_tag="ACHKBL_002490"
     CDS             2948..4153
                     /gene="rhmD"
                     /locus_tag="ACHKBL_002490"
                     /EC_number="4.2.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050032 - L-rhamnonate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnonate dehydratase"
                     /protein_id="HFO5819344.1"
                     /translation="MTLPKIKQVRAWFTGGATAEKGAGGGDYHDQGANHWIDDHIATP
                     MSKYRDYEQSRQSFGINVLGTLIVEVEAENGQTGFAVSTAGEMGCFIVEKHLNRFIEG
                     KCVSDIKLIHDQMLNATLYYSGSGGLVMNTISCVDLALWDLFGKVVGLPVYKLLGGAV
                     RDEIQFYATGARPDLAKEMGFIGGKMPTHWGPHDGDAGIRKDAAMVADMREKCGEDFW
                     LMLDCWMSQDVNYATKLAHACAPYNLKWIEECLPPQQYEGYRELKRNAPVGMMVTSGE
                     HHGTLQSFRTLSETGIDIMQPDVGWCGGLTTLVEIAAIAKSRGQLVVPHGSSVYSHHA
                     VISFTNTPFSEFLMTSPDCSTMRPQFDPILLNEPVPVNGRIHKSVLDKPGFGVELNRD
                     CNLKRPYNH"
     gene            4210..5499
                     /gene="yfaV"
                     /locus_tag="ACHKBL_002491"
     CDS             4210..5499
                     /gene="yfaV"
                     /locus_tag="ACHKBL_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416749.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO5819345.1"
                     /translation="MSTALLDAVVKKNRARLIPFMLALYVLAFLDRSNIGFAKQTYQI
                     DTGLSNEAYALGAGIFFVVYAFLGVPANLLMRKLGARTWIGTTTLLWGFLSAAMAWAD
                     TEAKFLIVRTLLGAAEAGFFPGMIYLTSQWFPQRNRASIMGLFYMGAPLALTLGSPLS
                     GALLEMHGFMGHPGWFWMFVIEGLLAVGAGVFTFFWLDDTPEQARFLSKEEKTLLINQ
                     LASEEQQKVTSRLSDALRNGRVWQLAIIYLTIQVAVYGLIFFLPTQVAALLGTKVGFT
                     ASVVTAIPWVAALFGTWLIPRYSDKTGERRNVAALTLLAAGIGIGLSGLLSPVLAIVA
                     LCVAAIGFIAVQPVFWTMPTQLLSGTALAAGIGFVNLFGAVGGFIAPILRVKAETLFA
                     SDAAGLLTLAAVAVIGSLIIFTLRVNRTVAQTDVAHH"
     gene            5516..6319
                     /gene="yfaU"
                     /locus_tag="ACHKBL_002492"
     CDS             5516..6319
                     /gene="yfaU"
                     /locus_tag="ACHKBL_002492"
                     /EC_number="4.1.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxy-L-rhamnonate aldolase"
                     /protein_id="HFO5819346.1"
                     /translation="MNALLTNPFKERLRKGEVQIGLWLSSTTAYMAEIAATSGYDWLL
                     IDGEHAPNTIQDLYHQLQAVAPYASQPVIRPVEGSKSLIKQVLDIGAQTLLIPMVDTA
                     DQARQVVSATRYPPYGERGVGASVARAARWGRIENYMAHVNDSLCLLVQVESKTALDN
                     LDEILDVEGIDGVFIGPADLSASLGYPDNAGHPEVQRIIETSIRRIRAADKAAGFLAV
                     APDMAQQCLAWGANFVAVGVDTMLYSDALDQRLAMFKSGKNGPRIKGNY"
     gene            complement(6360..6593)
                     /locus_tag="ACHKBL_002493"
                     /pseudo
     CDS             complement(6360..6593)
                     /locus_tag="ACHKBL_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311156.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6594..7589)
                     /gene="rpnE"
                     /locus_tag="ACHKBL_002494"
     CDS             complement(6594..7589)
                     /gene="rpnE"
                     /locus_tag="ACHKBL_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HFO5819347.1"
                     /translation="MTESTTSSPHDAVFKTFMFTPETARDFLEIHLPEPLRKLCNLQT
                     LRLEPTSFIEKSLRAYYSDVLWSVETSDSEGYIYCVIEHQSSAEKNMAFRLMRYATAA
                     MQRHLDKGYDRVPLVVPLLFYHGETSPYPYSLNWLDEFDDPQLARQLYTEAFPLVDIT
                     IVPDDEIMQHRRIALLELIQKHIRDRDLIGMVDRITTLLVRGFTNDSQLQTLFNYLLQ
                     CGDTSRFTRFIEEIAERSPLQKERLMTIAERLRQEGHQIGWQEGKIEGWQEGKIEGWQ
                     EGKLEGLQEGKLDGLQEGMHEQAIKIALRMLEQGFEREIVLATTQLTDADIPNCH"
     gene            complement(7782..8972)
                     /gene="glpC"
                     /locus_tag="ACHKBL_002495"
     CDS             complement(7782..8972)
                     /gene="glpC"
                     /locus_tag="ACHKBL_002495"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit GlpC"
                     /protein_id="HFO5819348.1"
                     /translation="MNDTSFENCIKCTVCTTACPVSRVNPGYPGPKQAGPDGERLRLK
                     DGALYDEALKYCINCKRCEVACPSDVKIGDIIQRARAKYDTTRPSLRNFVLSHTDLMG
                     SVSTPFAPIVNTATSLKPVRQLLDAALKIDHRRTLPKYSFGTFRRWYRSVAAQQAQYK
                     DQVAFFHGCFVNYNHPQLGKDLIKVLNAMGTGVQLLSKEKCCGVPLIANGFTDKARKQ
                     AITNVESIREAVGVKGIPVIATSSTCTFALRDEYPEVLNVDNKGLRDHIELATRWLWR
                     KLDEGKTLPLKPLPLKVVYHTPCHMEKMGWTLYTLELLRKIPGLELTVLDSQCCGIAG
                     TYGFKKENYPTSQAIGAPLFRQIEESGADLVVTDCETCKWQIEMSTSLRCEHPITLLA
                     QALA"
     gene            complement(8969..10228)
                     /gene="glpB"
                     /locus_tag="ACHKBL_002496"
     CDS             complement(8969..10228)
                     /gene="glpB"
                     /locus_tag="ACHKBL_002496"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001578690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HFO5819349.1"
                     /translation="MRFDTVIMGGGLAGLLCGLQLQKHGLRCAIVTRGQSALHFSSGS
                     LDLLSHLPDGQPVTDIHSGLEALRQQAPAHPYTLLGPQRVLDLACQAQALIAESGAQL
                     QGSVELAHQRVTPLGTLRSTWLSSPEVPVWPLPAKKICVVGISGLMDFQAHLAAASLR
                     ELDLAVETAEIELPELDVLRNNATEFRAVNIARFLDNEENWSLLLDALIPVANTCEMI
                     LMPACFGLADDKLWRWLNEKLPCSLMLLPTLPPSVLGIRLQNQLQRQFVRQGGVWMPG
                     DEVKKVTCKNGVVNEIWTRNHADIPLRPRFAVLASGSFFSGGLVAERDGIREPILGLD
                     VLQTATRGEWYKGDFFAPQPWQLFGVTTDEALRPSQAGQTIENLFAIGSVLGGFDPIA
                     QGCGGGVCAVSALHAAQQIAQRAGGQL"
     gene            complement(10218..11846)
                     /gene="glpA"
                     /locus_tag="ACHKBL_002497"
     CDS             complement(10218..11846)
                     /gene="glpA"
                     /locus_tag="ACHKBL_002497"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0046174 - polyol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HFO5819350.1"
                     /translation="MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIAT
                     GATGRNHGLLHSGARYAVTDAESARECISENQILKRIARHCVEPTNGLFITLPEDDLS
                     FQATFIRACEEAGISAEAIDPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLD
                     AKEHGAVILTAHEVTGLIREGATVCGVRVRNHLTGETQALHAPVVVNAAGIWGQRIAE
                     YADLRIRMFPAKGSLLIMDHRINQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYN
                     EIDDNRVTAEEVDILLREGEKLAPVMAKTRILRAYSGVRPLVASDDDPSGRNVSRGIV
                     LLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADLALPGSQEP
                     AEVTLRKVISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVEN
                     LNVNSLLDLRRRTRVGMGTCQGELCACRAAGLLQRFNVTMSAQSIEQLSTFLNERWKG
                     VQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL"
     gene            12119..13477
                     /gene="glpT"
                     /locus_tag="ACHKBL_002498"
     CDS             12119..13477
                     /gene="glpT"
                     /locus_tag="ACHKBL_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015169 - glycerol-3-phosphate
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HFO5819351.1"
                     /translation="MLSIFKPAPHKARLPAAEIDPTYRRLRWQIFLGIFFGYAAYYLV
                     RKNFALAMPYLVEQGFSRGDLGFALSGISIAYGFSKFIMGSVSDRSNPRVFLPAGLIL
                     AAAVMLFMGFVPWATSSIAVMFVLLFLCGWFQGMGWPPCGRTMVHWWSQKERGGIVSV
                     WNCAHNVGGGIPPLLFLLGMAWFNDWHAALYMPAFCAILVALFAFAMMRDTPQSCGLP
                     PIEEYKNDYPDDYNEKAEQELTAKQIFMQYVLPNKLLWYIAIANVFVYLLRYGILDWS
                     PTYLKEVKHFALDKSSWAYFFYEYAGIPGTLLCGWMSDKVFRGNRGATGVFFMTLVTI
                     ATIVYWMNPAGNPTVDMICMIVIGFLIYGPVMLIGLHALELAPKKAAGTAAGFTGLFG
                     YLGGSVAASAIVGYTVDFFGWDGGFMVMIGGSILAVILLIVVMIGEKRRHQELLQKRN
                     GG"
     gene            13482..14558
                     /gene="glpQ"
                     /locus_tag="ACHKBL_002499"
     CDS             13482..14558
                     /gene="glpQ"
                     /locus_tag="ACHKBL_002499"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFO5819352.1"
                     /translation="MKLTLKNLSMAIMMSGMIMGSSAMAADSNEKIVIAHRGASGYLP
                     EHTLPAKAMAYAQGADYLEQDLVMTKDDHLVVLHDHYLDRVTDVADRFPDRARKDGRY
                     YAIDFTLDEIKSLKFTEGFDIENGKKVQTYPGRFPMGKSDFRVHTFEEEIEFVQGLNH
                     STGKNIGIYPEIKAPWFHHQEGKDIAAKTLEVLKKYGYSGKDDKVYLQCFDADELKRI
                     KNELEPKMGMDLNLVQLIAYTDWNETQQKQPDGSWVNYNYDWMFKPGAMKQVAEYADG
                     IGPDYHMLIEETSQPGNIKLTGMVQDAQQNKLVVHPYTVRSDKLPEYTTDVNQLYDAL
                     YNKAGVNGLFTDFPDKAVKFLNKE"
     gene            14761..15411
                     /gene="inaA"
                     /locus_tag="ACHKBL_002500"
     CDS             14761..15411
                     /gene="inaA"
                     /locus_tag="ACHKBL_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA"
                     /protein_id="HFO5819353.1"
                     /translation="MAVSAKHDEFNHWWATEGDWVEEPNYRRNGMSGVQCVERNGKKL
                     YVKRMTHHLFHSVRYPFGRPTIVREVAVIKELERAGVIVPKIVFGEAVKIEGEWRALL
                     VTEDMAGFISIADWYAQHAVTPYSDEVRQAMLKAVALAFKKMHSVNRQHGCCYVRHIY
                     VKTEGKSEAGFLDLEKSRRRLRRDKAINHDFRQLEKYLEPIPKADWEQVKAYYYAM"
     gene            complement(15465..15719)
                     /gene="yfaE"
                     /locus_tag="ACHKBL_002501"
     CDS             complement(15465..15719)
                     /gene="yfaE"
                     /locus_tag="ACHKBL_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like diferric-tyrosyl radical
                     cofactor maintenance protein YfaE"
                     /protein_id="HFO5819354.1"
                     /translation="MARVTLRITGTQLLCQDEHPSLLAALESHNVAVEYQCREGYCGS
                     CRTRLVAGQVDWIAEPLAFIQPGEILPCCCRAKGDIEIEL"
     gene            complement(15719..16849)
                     /gene="nrdB"
                     /locus_tag="ACHKBL_002502"
     CDS             complement(15719..16849)
                     /gene="nrdB"
                     /locus_tag="ACHKBL_002502"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ia ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFO5819355.1"
                     /translation="MAYTTFSQTKNDQLKEPMFFGQPVNVARYDQQKYDIFEKLIEKQ
                     LSFFWRPEEVDVSRDRIDYQALPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLISI
                     PELETWVETWAFSETIHSRSYTHIIRNIVNDPSVVFDDIVTNEQIQKRAEGISSYYDE
                     LIEMTSYWHLLGEGTHTVNGKTVTVSLRELKKKLYLCLMSVNALEAIRFYVSFACSFA
                     FAERELMEGNAKIIRLIARDEALHLTGTQHMLNLLRSGADDPEMAEIAEECKQECYDL
                     FVQAAQQEKDWADYLFRDGSMIGLNKDILCQYVEYITNIRMQAVGLDLPFQTRSNPIP
                     WINTWLVSDNVQVAPQEVEVSSYLVGQIDSEVDTDDLSNFQL"
     gene            complement(16915..19200)
                     /gene="nrdA"
                     /locus_tag="ACHKBL_002503"
     CDS             complement(16915..19200)
                     /gene="nrdA"
                     /locus_tag="ACHKBL_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1a ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFO5819356.1"
                     /translation="MNQNLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELR
                     SHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPAL
                     YDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKYLVQNR
                     VTGEIYESAQFLYILVAACLFSNYPRETRLQYVKRFYDAVSTFKISLPTPIMSGVRTP
                     TRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMD
                     YGVQINKLMYTRLLKGEDITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQR
                     VKAVELFSLMMQERASTGRIYIQNVDHCNTHSPFDPAIAPVRQSNLCLEIALPTKPLN
                     DVNDENGEIALCTLSAFNLGAINSLDELEELAILAVRALDALLDYQDYPIPAAKRGAM
                     GRRTLGIGVINFAYYLAKHGKRYSDGSANNLTHKTFEAIQYYLLKASNELAKEQGACP
                     WFNETTYAKGILPIDTYKKDLDAIANEPLHYDWEALRESIKTHGLRNSTLSALMPSET
                     SSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLV
                     GIMQKFIDQSISANTNYDPSRFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDGAED
                     AQDDLVPSIQDDGCESGACKI"
     gene            19328..19471
                     /gene="ypaB"
                     /locus_tag="ACHKBL_002504"
     CDS             19328..19471
                     /gene="ypaB"
                     /locus_tag="ACHKBL_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001753443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpaB"
                     /protein_id="HFO5819357.1"
                     /translation="MALLQVHNFVDNSGRKKWLSRTLGQTRCPGKSMGREKFVKNNCS
                     VIS"
BASE COUNT         4459 a         4855 c         5324 g         4833 t
ORIGIN      
        1 tttttcgctc attgaatcct ctgcgccagg cagtacaatc ccgcagcttt gggaattttg
       61 attaattgct tgtgggaaag gggataatga aaaaaattgc gctagcaggt ctggccggaa
      121 tgctgttggt ttctgcatcg gtcaatgcaa tgagcatcag cggccaggcg ggtaaagaat
      181 acaccaatat tggtgtcggt tttgggactg aatcgacggg cctggcgtta agcggtaact
      241 ggacacataa cgacgacgac ggtgacgtcg cgggcgtggg gctggggttg aatctgcctc
      301 tcgggccgtt aatggcgacc gttggcggaa aaggcgttta caccaacccg aattacggcg
      361 atgaaggtta tgccgcagcg gtaggaggtg gtttgcagtg gaaaattggc aacagcttcc
      421 gcttgtttgg cgagtattac tactctccgg attcgctctc cagcggtatt caaagttatg
      481 aggaagcgaa tgctggcgcg cgttacacca ttatgcgtcc agtcagtatt gaggcgggtt
      541 atcgctacct gaacctttct ggtaaagacg gtaaccgcga caacgcagtg gctgacggcc
      601 cgtatgtggg ggttaacgcc agtttttgat cccttccggc gcggcattct gccgcgccat
      661 tcagtttctt actacgcctt atctcccttt gcgttatagt gattcgataa caaaagcagg
      721 agataaccat gttaaaagtg gaaatgttat ccaccgggga tgaagtgtta cacgggcaaa
      781 tcgttgacac taacgctgcc tggctggccg attttttctt tcatcagggg ttgcctttaa
      841 ctcgccgcaa tacggtgggg gataaccttg atgacttagt gaccattctt cgcgaacgga
      901 gtcagcacgc cgatgtgctg atcgttaacg gcgggctggg gccgaccagt gatgatttaa
      961 gcgcactcgc cgctgcgaca gcaaaaggtg aaggcctggt gctgcatgag gcctggctca
     1021 aagagatgga acgctatttc cacgaacgtg gacgagtaat ggcaccgagc aaccgtaaac
     1081 aagcggagct gcctgccagt gctgaattta tcaataaccc ggtaggcacc gcctgtggtt
     1141 ttgccgtgca gcttaatcgt tgcctgatgt tctttacccc cggcgtaccg tcagaattta
     1201 aagtgatggt cgagcacgaa atcctgccgc gcctgcgcga gcgtttttct ttaccgcagc
     1261 cgccggtttg tctgcgtttg actacatttg gtcgttcgga aagcgatctg gcacaaagcc
     1321 tggacaccct gcaactgccg ccgggcgtaa caatgggcta tcgctcttct atgccgatta
     1381 tcgaactgaa actcaccgga ccggcaagcg agaaacaggc gatggaaaaa ctgtggctgg
     1441 acgtcaaacg tgttgccgga cagagcgtga ttttcgaagg taccgaagga ctgccctcgc
     1501 agatcagccg cgaattgcaa acccgccagt tcagcctgac gttgagcgag caattcacag
     1561 gtggtttatt ggctttgcaa ctttctcgcg caggtgctcc attgctggcg tgtgaagtgg
     1621 ttccttcaca ggaggaaacc ctggcgcaaa ctgcgcactg gattacagaa cggcgggcca
     1681 accattttgc cgggctggca ctggctgttt cgggtttcga gaacgagcat ctcaactttg
     1741 cgctagccac gccagatggc actttcgctc tgcgtgtgcg tttcagcact acacgctaca
     1801 gcctggctat ccgtcaggaa gtgtgcgcaa tgatggcact gaatatgctg cgccgttggt
     1861 taaacggcca ggacatcgcc agtgagcatg gctggattga ggttgttgag tccatgacct
     1921 tatctgtctg attccgccac tctgtgccgg tttactgcac cggcacattc cccctccggc
     1981 aaaatttgtc caacctgttg tactcaataa gaaaaaatcc gtgcgctaat tcacaaattt
     2041 gtgcgtaatt tctccagtcc gctggacgag gagcgggatt tgatgagctt cacaatttta
     2101 ctccacctga gcaacgagac tgattccagt atctaagact ggagtccagt atgttggaat
     2161 caagcaaagt tcccgccctg acacgggcta ttgatattct caatttgatt gcccgcattg
     2221 gcccctgtag tgctgcgacg atcattgaca cattggggat ccctaaaagc acggcctatt
     2281 tgctgcttaa tgaactcagg cgtcagcgtt ttcttagtct tgatcaccag gaaaattttt
     2341 gtttgtggac caggctggtg gagctttccg gtcatgcgtt aagcaagatg gatctccggg
     2401 aactggctcg accgcgtttg acgcaactga tggataccac cgggctgtta tgtcacctgg
     2461 ggattattga taacggcagc gcttattaca ttttgaaagt ggagtcatca gccactatca
     2521 gcgtgcgttc ccatgaagga aaaagccttt cgctttatcg ctccggaata ggtaaatgcc
     2581 tgcttgcctg gcaacctgca gctgtacagc aaagtattat cgaagggctg atctgggagc
     2641 aggcgacacc cacaacgatt actcatccac agcaacttca cgaagaactg gcgcgtattc
     2701 gccgtcaggg ctggagctat gacaacggcg aagattacgc cgatgtccgt tgtgtcgctg
     2761 cgccggtgtt taacgccaat aacgagctta ccgctgcgat ctcggtggtc ggtacccgct
     2821 tacaaatcaa tgaagaatac cgtgattatc tggcgggcaa ggccattgcc tgcgccaggg
     2881 atatttcacg tctgttggga tggaaaagtc cctttgactt acaggcctca taacaatgga
     2941 gaacagcatg accctaccaa aaatcaaaca ggttcgcgcc tggtttactg gcggtgcgac
     3001 agcagaaaaa ggcgctggcg gcggtgatta tcacgaccag ggggcaaatc actggattga
     3061 tgatcatatt gccaccccga tgagtaaata ccgcgattac gagcagtcac gccagtcatt
     3121 tggcattaac gttcttggca cgttgattgt tgaagtcgaa gcagaaaacg gccagaccgg
     3181 attcgccgtt tcgacagccg gtgaaatggg ctgttttatt gtcgaaaaac accttaaccg
     3241 tttcattgag ggtaaatgtg tcagtgatat caaactgatc cacgatcaaa tgctcaatgc
     3301 caccctgtat tactccggct ctggtggcct ggtgatgaac acgatttcct gtgtcgatct
     3361 ggctctgtgg gatctgttcg gcaaagtggt cgggcttccg gtttataaac ttttaggcgg
     3421 cgcagttcgt gatgagattc agttctacgc cacaggtgcg cgtccggatc tggcaaaaga
     3481 gatgggcttt atcggtggca aaatgccgac gcactggggg ccacatgatg gcgatgcggg
     3541 gatccgcaaa gatgccgcta tggtcgcgga tatgcgtgaa aaatgcggtg aggatttctg
     3601 gttaatgctc gactgctgga tgagtcagga cgtgaactat gccaccaaac tggcccacgc
     3661 ttgcgcgccc tataacctga aatggatcga agagtgcctg ccgccacagc agtatgaagg
     3721 ttatcgcgaa ctgaaacgca acgcgccagt cggaatgatg gtcaccagcg gtgagcatca
     3781 cggcacactg caatctttcc gtacgctttc agaaaccggt atcgacatta tgcagccgga
     3841 tgttggctgg tgcggtggct taaccacgct ggtggaaatt gccgcaatcg ccaaatccag
     3901 agggcaactg gtggtgccgc acggttcgtc tgtttactct caccatgcgg tgatcagctt
     3961 caccaatacg ccattcagcg aattcctgat gaccagcccg gattgttcaa cgatgcgtcc
     4021 gcaatttgat ccgattctgc ttaatgagcc agtgccggtg aatggtcgta ttcataaatc
     4081 agtgcttgat aaacccggtt tcggcgtcga actcaatcgt gactgcaatt tgaaacgccc
     4141 ctacaaccac taatcactcc gggcgttgct gtgacaacgg taacgcccac atattctaag
     4201 gagcttttta tgagcaccgc tttgcttgac gccgtggtga agaaaaaccg tgcgcgttta
     4261 attccgttta tgttggcgct gtatgtgctg gcgtttctcg accgttcgaa tatcggtttt
     4321 gccaaacaga cctaccagat tgataccggg ctgagtaatg aagcttatgc gctgggagca
     4381 ggcattttct ttgtggtata tgcgtttcta ggtgttccgg cgaatctttt gatgcgcaaa
     4441 ctgggggcca gaacctggat tggtacgaca acactgctgt ggggatttct ttcggcagcc
     4501 atggcatggg ccgatactga agcgaaattt ctgatcgttc gcactctgct tggtgctgcg
     4561 gaggccgggt tcttccctgg tatgatttat ctcacctcgc aatggtttcc gcagcgtaat
     4621 cgcgccagca ttatggggct gttctatatg ggcgcaccgt tggcgttaac actgggatca
     4681 ccgctttccg gcgcgctgct ggagatgcat ggatttatgg gacatcccgg ctggttctgg
     4741 atgtttgtga ttgaaggatt gctggcagtc ggcgctgggg tattcacatt cttttggctt
     4801 gatgacacac cggagcaggc acgttttctg agtaaagaag aaaaaacatt gcttatcaat
     4861 caactggcaa gtgaagaaca acagaaagtg acttctcgac tgagcgatgc gctgcgtaat
     4921 gggcgagtct ggcaactggc gattatctac ctgaccattc aggtggcggt ttacggatta
     4981 attttcttcc tgccgaccca ggttgctgca ttgctgggga caaaagtggg ctttacggcg
     5041 tcggtggtca ccgccattcc gtgggttgcg gccttgtttg ggacctggct tattccgcgc
     5101 tattccgata aaaccggcga acggcgtaat gtcgcagcgc tgacattact ggcggcaggc
     5161 attggtattg gtctctccgg gctgctttct ccagtactgg cgatcgtagc gctgtgtgtt
     5221 gcagccatcg ggtttattgc cgtgcagccg gtgttctgga cgatgccgac acaactgctt
     5281 tccggtacgg cgctggcggc ggggattggt tttgtaaacc tgtttggtgc agtgggtggg
     5341 tttattgccc cgatcctgcg cgtgaaagca gaaacgttgt ttgccagcga tgcggcggga
     5401 ttactgacgc tggcagcggt ggcggtcatc ggttcgctga ttattttcac tctgcgtgta
     5461 aatcgcactg ttgcgcagac cgacgtggca catcattaat aggttaagga acacgatgaa
     5521 cgcattatta accaatccct ttaaagaacg tttacgcaag ggcgaagtgc aaattgggct
     5581 gtggttaagc tcaacgactg cctatatggc agaaattgcc gctacttccg gttatgactg
     5641 gttgctgatt gacggggagc acgcgccaaa caccattcag gatctttatc accagctaca
     5701 ggcggtagcg ccctatgcca gccaacccgt gatccgtccg gtggaaggca gtaagtcgct
     5761 gattaaacaa gtcctggata ttggcgcgca aactctactg atcccgatgg tcgatactgc
     5821 cgatcaggca cgtcaggtgg tgtctgccac gcgctatcct ccctacggtg agcgtggtgt
     5881 cggggccagt gtggcacggg ctgctcgctg gggacgcatt gagaattaca tggcgcacgt
     5941 caacgattcg ctttgtctgt tggtgcaggt ggaaagtaaa acggcactgg ataacctgga
     6001 cgaaatcctc gacgtcgaag ggattgatgg cgtgtttatt ggacctgcgg atctttctgc
     6061 gtcgttgggc tacccggata acgccgggca cccggaagtg cagcgaatta ttgaaaccag
     6121 cattcggcgg atccgtgctg cggataaagc ggctggtttt ctggctgtgg ctcctgatat
     6181 ggcgcagcaa tgcctggcgt ggggagcgaa ctttgtcgct gttggcgttg acacgatgct
     6241 ctacagcgat gccctggatc aacgactggc gatgtttaaa tcaggcaaaa atgggccacg
     6301 cataaaaggt aattattgat atcaaaggcc catgggggtc ggctgtgggc ctgtgttaat
     6361 tagtggttat tcgctgccag atcggcttcg cttagctggg tggctgcgag caccaggtca
     6421 cgatcaatgc cctgtaccag catgcgcaaa gcaattttaa tggcttgttt acgcatacct
     6481 tcctgcaaac cttctaattt accctcctgc cagccttcaa gcttaccttc ctgccagcca
     6541 atctgatgcc cttcctgcct cagccgttca gcaatagtca tcaatctctc cttttaatga
     6601 cagttcggaa tatcagcatc agtgagttgg gttgtcgcca gcacaatctc acgttcaaag
     6661 ccctgctcca gcatgcgcaa ggcaatttta atggcttgtt catgcatgcc ttcctgcaaa
     6721 ccatctaatt taccttcttg caaaccttct aatttacctt cctgccaacc ttcaatttta
     6781 ccttcctgcc agccttcaat cttaccttcc tgccagccaa tttgatgccc ttcctgccgc
     6841 agccgttcag caatagtcat taatctctcc ttttgtagtg gtgaacgttc ggcaatctcc
     6901 tcaataaaac gggtgaaacg ggaggtatcg ccgcattgca gcagataatt aaacagcgtt
     6961 tgtagctggc tgtcattagt gaagcctcta accaaaagcg tggtgatcct gtcgaccata
     7021 ccgattaaat cgcggtcgcg aatatgcttt tgaatcagtt caagcagagc tatacgccga
     7081 tgttgcatga tctcatcgtc aggtacgatg gtgatatcca ccaacggaaa agcttcggtg
     7141 tacaactgcc gggcaagttg cggatcgtca aactcatcca gccagttgag tgagtacggg
     7201 tagggcgagg tttcgccatg ataaaacagc aatggcacca ccagcggaac tctgtcatag
     7261 cctttatcca ggtgacgctg catggcggca gtggcatagc gcattagccg aaaagccata
     7321 ttcttttctg cagagctttg atgttcaatc acgcagtaga tatagccttc actgtcgctg
     7381 gtttccacag accacaaaac atccgagtag taagcgcgta aacttttttc aataaagctg
     7441 gtgggttcca ggcgtaaagt ttgcaggttg caaagcttgc gcagcggctc tggtagatgt
     7501 atttccagaa aatcccgagc ggtttcgggt gtgaacataa aggttttaaa caccgcatca
     7561 tgcggggagg aggttgttga ttcggtcatg gttgtccgtc acctgatatt tatgatgacg
     7621 cgacattacg gttatcccag gcctttgaca tctgccagtt tcagcctttg cgagacgttt
     7681 tcagaggaat tttcactgcg ttgcaacgca tacattgggc acggaatcgt gtacgcaaaa
     7741 atgcccggta acaacatgct taccgggcat cagaaaggag tttaagccag cgcctgggcc
     7801 agcagcgtaa tcggatgttc gcagcgaaga cttgtggaca tctcaatctg ccatttgcag
     7861 gtttcgcagt cggtaaccac cagatctgcg ccgctttctt ctatctgacg gaacagtggt
     7921 gcgccgatgg cttgtgaggt ggggtagttc tcttttttga aaccgtaagt acctgcaata
     7981 ccgcagcact gggaatccag caccgttaac tcaagccccg ggattttacg caacagctcc
     8041 agggtgtaga gcgtccagcc cattttttcc atatggcacg gagtgtgata aaccactttc
     8101 agcggcagcg gtttcagcgg caaggttttg ccttcgtcca gcttgcgcca cagccagcgg
     8161 gtcgccagtt cgatgtgatc gcgcaagcct ttgttgtcga cattcagcac ttccggatat
     8221 tcgtcgcgca gggcaaatgt acaggttgag gaggtggcaa tcaccggaat gccttttact
     8281 cccactgctt cgcggatcga ctctacattt gtgattgcct gtttacgtgc tttatcggta
     8341 aagccgttgg cgatcagcgg tacgccgcag catttttctt tgctgagcag ttgtacaccg
     8401 gtacccattg cgttgagcac tttaattaaa tctttgccta actgcggatg gttgtagtta
     8461 acgaagcagc cgtgaaagaa agccacctgg tctttatatt gtgcctgctg ggccgccacg
     8521 ctgcgatacc agcgacggaa cgtaccgaag gagtatttcg gtagcgtgcg gcgatgatcg
     8581 atttttaacg ccgcatcaag cagctgccgc actggtttca gcgaggtagc ggtattgacg
     8641 attggtgcga atggcgtgga aacgctaccc atcaggtcgg tatgactcaa cacaaaattg
     8701 cgcagcgacg ggcgtgtggt gtcatatttc gcccgcgcgc gctggatgat atcgccaatc
     8761 ttcacatcgg acgggcaggc gacttcgcag cgtttgcagt tgatgcaata tttcagcgct
     8821 tcgtcataca gtgcgccatc tttcagacgc agacgctcgc catccggccc ggcttgtttt
     8881 ggccctggat agccgggatt cacccggctc accgggcagg cggtggtgca gacggtgcac
     8941 ttaatgcagt tttcgaagct ggtgtcgttc atagttggcc tcctgcgcgt tgggcaatct
     9001 gttgagcggc atgtaaagca ctgacggcac aaacaccgcc gccgcatccc tgggcgatgg
     9061 gatcaaatcc gcccagcacc gaaccgatgg caaacaggtt ctcaatggtt tgccctgcct
     9121 gcgatgggcg tagtgcctca tcagtggtta caccgaacag ctgccacggt tgcggcgcaa
     9181 aaaaatcgcc cttataccat tcaccccgcg tggcggtttg tagcacatca aggccgagaa
     9241 tcggctcgcg aatgccgtca cgttctgcca ccagtccgcc gctaaagaaa ctgccgctcg
     9301 caagcaccgc aaatcgtggg cgcagcggga tatcggcgtg attgcgcgtc cagatttcat
     9361 tcactacgcc atttttacag gtcacttttt tcacttcatc gcctggcatc cacacgccac
     9421 cctggcgcac aaactggcgc tgtaactggt tttgcagacg aatgcccagc acggaaggcg
     9481 gcagcgttgg caaaagcatc agtgaacaag gtagtttttc attcaaccaa cgccacagtt
     9541 tgtcatcggc caggccgaag caggcgggca tcaggatcat ttcgcaggta ttggcgacag
     9601 gaataagcgc atcaagtaac agcgaccagt tctcttcgtt atcaaggaaa cgggcgatat
     9661 tcaccgcgcg aaactcggtg gcgttgttgc gcagcacatc cagttccggc agctctattt
     9721 ctgcggtttc aacggcaagg tcgagttcgc gcaacgaagc cgccgcaaga tgcgcctgaa
     9781 aatccatcag gccgcttatc ccgaccacac atattttctt cgcgggcaac ggccagacag
     9841 gaacttctgg cgaacttagc caggttgagc gcagagtgcc gagcggggta actcgctggt
     9901 gggccagttc tacgctgccc tgtaattgcg caccgctttc agcgattaat gcctgcgcct
     9961 ggcaagcgag atcgagtacg cgttgtggcc cgagaagagt gtaaggatgg gcaggtgcct
    10021 gctgacgcaa agcttccagt ccactgtgaa tgtctgtcac cggttgacca tccggcagat
    10081 ggctcagcaa atccagcgat ccagatgaga aatggagcgc gctttgacca cgcgtgacaa
    10141 tggcacagcg caggccgtgt ttttgcagtt gcaggccaca gagtaatccg gcgaggccgc
    10201 cgcccataat gacagtatca aagcgcatct ttctgctcct tctccagacc acacagtccc
    10261 tgataaaccc agcgggtaaa ttcgctttcg cgcagtgcat ctccccaggc gatgggttgc
    10321 acgcctttcc agcgttcgtt aaggaaggtg gaaagttgct cgatagattg cgcggacatc
    10381 gtgacgttaa aacgttgcag cagtccggca gcgcggcagg cgcagagttc gccctggcag
    10441 gtacccatcc ccacacgggt acgacggcgt aaatccagca ggctattaac gtttaaattt
    10501 tctaccgcgt actgaacttc acccgcagtt accgcttcgc actcacatac caggctacgg
    10561 tgcagacggc cttcgctcag ccaggcgggt gtgcgatcgc catgacgata aaccgcagaa
    10621 ccgcgcagcg gggcaggcag ggagatgact ttacgcaagg taacttcagc gggctcctgt
    10681 gaaccaggaa gtgccagatc ggcggttgta caggggcgcg tgttgcccag cttgcgacat
    10741 accgcgtcgg tagcccattc cgccatcagc cgataggtca tcagtttgcc gccggtgatg
    10801 gtgataaatc cgtccagacc atcgcgttca gcatggtcga gcagcacgat gccacgactg
    10861 acgttacggc cgctcgggtc gtcatcgctg gcaaccagcg gacgcacgcc agagtaagcc
    10921 cgcaaaatac gcgttttcgc catcacgggg gccagttttt ccccttcacg cagcagaata
    10981 tcaacttctt ctgccgtcac tcgattatcg tcaatctcat tgtaatcaat acgtaaagag
    11041 gtggtgccaa ttagcgaaat ggtatcgcca ggcaccagaa tatcggcgtc ggaaggtttg
    11101 cggcagcggt tgatcacatg ctgattaatg cggtgatcca tgatcagcag cgatcctttc
    11161 gccgggaaca tgcgaatgcg cagatcggca tattcggcaa tgcgttgccc ccagatccca
    11221 gcggcattaa ccacgacagg cgcatgaagg gcctgagttt cgccggtgag atggttacgt
    11281 acacgaacgc cgcacaccgt cgcaccttca cgaatcagcc ccgtgacttc atgagcggta
    11341 aggataacgg caccgtgttc tttggcatcc agcatgtttg ctgcggtcag acgaaatgga
    11401 tcaacggtgc catccggaac tttcaccgcg ccaatcagtg ccgggttaac ggcaggttcg
    11461 ataatgcgcg cttgctgcgg gtctatagct tctgcgctga tccctgcttc ttcgcaggcg
    11521 cggataaaag tggcctggaa ggagaggtca tcttccggca gggtgataaa caggccgttg
    11581 gtcggttcaa cgcagtgacg tgcaatgcgt ttcaggatct ggttttcact aatacattcg
    11641 cgggccgatt ccgcatcggt taccgcatag cgcgcaccgc tgtgcagcag gccgtggtta
    11701 cgcccggtgg caccggttgc gatgtcgtgg cgctcaacca aaatcacgcg cagtccgcgc
    11761 agggcacagt cgcgggcaat cccggctccc gttgcgccgc cgccaatgat aatcacgtca
    11821 cttgattgcg agtcgcgagt tttcattgtt atccctctga agttcgtttt ttaccattta
    11881 gccatagtaa aaacatgaat tgtttgattt cgcgcatatt cgctcataat tcgaaagtga
    11941 aacgtgattt catgcgtcat tatgaacatt taacccatcg aatttaataa tgtgtgcggt
    12001 aattcacatt taatttatga atgttttctt aacatcgcgg caactcaaga aacggcaggt
    12061 tctcccactg aatcaggctg ttaatcataa ataagaccac gggccacgga ggctatcaat
    12121 gttgagtatt tttaaaccag cgccacacaa agcgcgctta cctgccgcgg agatcgatcc
    12181 gacttatcgt cgattgcgct ggcaaatttt cctggggata ttctttggtt atgctgctta
    12241 ctatttggtt cgtaagaact ttgccctcgc tatgccttat ctggtcgagc agggattctc
    12301 acgcggtgat ttaggttttg ccctttcggg gatctcgatt gcttatggat tttcgaaatt
    12361 catcatgggc tcggtgtcgg atcgctcgaa tccgcgcgtt ttcctgcccg caggtttgat
    12421 tctggcggcg gcggtgatgc tttttatggg ctttgtgccg tgggcgacgt cgagcattgc
    12481 ggtgatgttt gtgctgctgt tcctctgcgg ttggttccag gggatgggat ggccgccgtg
    12541 cggtcgtact atggtgcact ggtggtcgca gaaagaacgt ggcggcattg tgtcagtgtg
    12601 gaactgtgcg cataacgtcg gtggtggtat tccgccgctg ctgttcctgc tggggatggc
    12661 ctggttcaat gactggcacg cggcgctcta tatgcccgct ttctgcgcca ttctggtggc
    12721 actgttcgcc tttgcgatga tgcgcgatac cccgcaatcc tgtggcctgc cgccgatcga
    12781 agagtacaaa aatgattatc cggacgacta taacgaaaaa gcggaacagg agctgacggc
    12841 gaagcaaatc ttcatgcagt acgtgctgcc gaacaaactg ctgtggtaca tcgctatcgc
    12901 caacgtgttc gtttatctgc tgcgttacgg catcctcgac tggtcaccga cttatctgaa
    12961 agaggtgaaa cacttcgcgc tggataaatc ctcctgggcc tacttcttct atgagtatgc
    13021 gggcattccg ggcaccctgc tgtgcggctg gatgtcggat aaagtcttcc gtggcaaccg
    13081 tggggccacc ggcgtcttct ttatgacact ggtgaccatc gcgactatcg tttactggat
    13141 gaacccggca ggtaacccaa ccgtagatat gatttgtatg attgttatcg gcttcctgat
    13201 ctacggccct gtgatgctga tcggtctgca tgcgctggaa ctggcaccga aaaaagcggc
    13261 aggtacggca gcgggcttta ccgggctgtt tggttacctg ggcggttcgg tggcggcgag
    13321 cgcgattgtt ggctacacag tggacttctt cggctgggat ggcggcttta tggtaatgat
    13381 tggcggcagc attctggcgg ttatcttgtt gattgttgtg atgattggcg aaaaacgtcg
    13441 ccatcaggaa ttactgcaaa aacgcaacgg aggctaatgg catgaaattg acgctgaaaa
    13501 accttagcat ggcgatcatg atgagtggaa tgatcatggg aagcagtgca atggcggcgg
    13561 acagcaacga aaaaatagtc atcgcccatc gcggtgccag tggatatttg ccggagcata
    13621 cgctgccagc aaaagcgatg gcgtatgcgc agggagcgga ttatctggaa caagatttgg
    13681 tgatgaccaa agacgaccat ctggttgtcc tgcatgacca ttatctcgat cgtgttactg
    13741 atgttgccga tcgtttcccg gatcgggcgc gcaaagacgg tcgttactac gcgatagatt
    13801 tcacgctcga tgaaattaag tcactgaaat ttaccgaagg tttcgatatt gaaaatggta
    13861 agaaagtaca gacttatccg gggcgtttcc cgatgggtaa atctgacttc cgggtacaca
    13921 ctttcgagga agagattgaa tttgttcagg ggttaaatca ctctaccggg aaaaatatcg
    13981 gtatctatcc agaaattaaa gcgccgtggt tccatcatca ggaagggaag gatattgcgg
    14041 caaaaacgct ggaagtgctg aagaaatatg gttacagcgg taaggacgac aaagtttatt
    14101 tgcaatgttt tgatgccgat gaactgaagc gtattaagaa tgagctggaa cctaaaatgg
    14161 gcatggacct caatctggta cagctgattg cctataccga ctggaacgaa actcagcaga
    14221 aacagccgga cggaagctgg gttaattaca actacgactg gatgtttaag ccgggggcca
    14281 tgaaacaggt ggcggaatat gctgatggta ttggtccgga ttatcatatg ttgattgagg
    14341 agacatcgca gccaggtaat atcaaactca ctggcatggt gcaagatgct cagcagaaca
    14401 agctggtagt gcatccttat accgtgcggt cagataaact gcctgaatac actactgatg
    14461 tgaatcagtt atatgatgct ctgtataaca aagcgggtgt aaatgggcta tttactgatt
    14521 tccctgataa ggcagtaaaa ttccttaata aagagtaaat taactaccgg atggcactta
    14581 tgtgctatcc gataccactc ttcattaaag tctgtgggtt atccttaatt gcaattattt
    14641 tgagcgagat gacttgttcc cggcataaga tactcgtcaa tacaacacgt attattaaga
    14701 ttttcctcag ctaaacatct atagtcattg tcgttaataa aaaacgacag gcgatagggt
    14761 atggcagttt cagcaaagca cgacgaattt aatcactggt gggcaacaga gggcgactgg
    14821 gttgaagagc ctaactatcg ccgtaatggt atgagcgggg tgcaatgcgt cgagcgcaac
    14881 ggcaaaaagc tatatgtaaa gcgcatgacg catcatctgt ttcattccgt acgttatccg
    14941 ttcggccgac caacgattgt ccgtgaggtt gcggtaatta aagaactgga acgggccggt
    15001 gtcatcgtgc cgaagatcgt ttttggtgaa gcggtgaaaa ttgaggggga atggcgcgcg
    15061 ttgctggtga ctgaagatat ggcggggttc atcagcattg ctgactggta tgcccagcat
    15121 gcagtaacgc cctattctga cgaagtacgg caagccatgt tgaaagcagt ggcgctggcg
    15181 tttaagaaaa tgcatagcgt taatcgtcag catggctgtt gctatgttcg ccatatctat
    15241 gtgaaaacag aaggtaagtc agaagctggt tttcttgatc tggagaaaag tcgtcgccgc
    15301 ttgcgtcgcg ataaagcgat taatcatgac ttccgccagc tggaaaaata tctggagccg
    15361 atccctaaag cggactggga gcaggtgaaa gcgtattact acgccatgta atcgttgtag
    15421 gccagataat acgcgtaagc gtcgtatctg gcgctagtac ccgatcacaa ctcgatttca
    15481 atatcgcctt ttgcccggca acaacagggc aaaatttccc ccggctgaat aaaggctaac
    15541 ggttcggcaa tccagtccac ctggcctgca acaaggcgcg tgcgacagga accgcagtaa
    15601 ccttcgcggc actggtactc aaccgccaca ttgtgggatt ccagcgccgc cagaagggaa
    15661 gggtgttcat cctggcacag cagttgtgtg ccagtgatgc gcagggtaac gcgagccatc
    15721 agagctggaa gttactcaaa tcgtcggtgt ccacttccga gtcaatctgc ccgaccagat
    15781 aagaactgac ttccacttcc tgcggagcaa cctgcacgtt atcagacacc agccaggtat
    15841 tgatccacgg gatcgggttg gagcgtgtct ggaacggcag atccaaaccg actgcctgca
    15901 tgcggatatt ggtgatgtat tcaacgtact ggcagagaat gtctttattc agaccaatca
    15961 tcgaaccgtc acggaacaga taatccgccc agtctttctc ttgctgtgcc gcctggacaa
    16021 acaggtcata gcactcctgc ttacattctt cggcaatttc cgccatctcc ggatcatccg
    16081 cgccgctgcg cagcagattc agcatatgct gagtaccggt caggtgcagg gcttcatcgc
    16141 gagcaatcag gcgaataatt ttggcgttac cttccatcag ttcgcgttct gcaaaggcaa
    16201 aggaacaagc aaagctgacg tagaagcgga tcgcttccag cgcgttaacg ctcatcaggc
    16261 agagatacag ttttttcttc agctcgcgca ggctaacggt tacggtttta ccgttaacgg
    16321 tgtgggtacc ttcgcccagc agatgccagt agctggtcat ttcgatcagc tcatcgtaat
    16381 agctggagat cccttccgca cgtttctgga tctgctcgtt ggtgacgata tcgtcaaaca
    16441 caacagacgg atcgttaacg atattacgaa tgatatgggt ataagaacga gagtgaatgg
    16501 tttctgagaa cgcccaggtt tcgacccagg tttccagctc agggatcgag atcagcggca
    16561 gcagtgccac gttcgggctg cgaccctgaa tggagtccag cagcgtctga tatttcaggt
    16621 tgctgataaa gatgtgtttt tcgtgttcag gtagtgcctg gtaatcgata cggtcacgag
    16681 aaacgtcaac ttcttccgga cgccagaaga aagagagctg cttttcgatc agcttttcga
    16741 agatgtcata tttttgctga tcgtagcgag ccacgttaac cggctgacca aagaacatcg
    16801 gttctttcag ctgatcattt ttcgtctgtg aaaaggtggt atatgccatg aatgtgtcct
    16861 gttgggagtt taatgccgga taaggcattt tacgccgcat ccggcatctc aatatcagat
    16921 cttacatgcg ccgctttcgc agccatcgtc ctggattgac ggtaccagat cgtcttgtgc
    16981 gtcttcagca ccgtcacggg tgttctgata atacagcgtt ttgaccccga atttgtaggc
    17041 ggtgagcagg tctttcagca gttgctgcat cggcactttt cctgacggga agcgtgacgg
    17101 atcgtagttg gtgttagcag agatcgactg atcgataaat ttctgcatga tacccaccag
    17161 ttgcaggtag ccatcgttac ccggcatttc ccacagcagt tcataggcgt cgtgcagatg
    17221 ctcgtagtcc ggcaccacct ggcgcagaat accgtctttc gacgctttga tgctgacgta
    17281 accacgcggc ggttcaatac cgttagtggc gttagagatc tgcgaagagg tctcggacgg
    17341 catcagggca gaaagcgtgg agttacgcag gccgtgcgtt ttgattgact cacgcagtgc
    17401 ttcccagtcg taatgcagcg gctcattagc gatggcatcc agatctttct tataggtatc
    17461 gatcggcagg atccctttcg cgtaagtggt ttcgttaaac cacgggcacg cgccttgctc
    17521 tttcgccagc tcattagacg ctttcagcag gtaatactga atggcttcga aggttttatg
    17581 cgtcaggttg ttggcgctgc cgtcggagta gcgtttaccg tgcttcgcca ggtagtaagc
    17641 gaagttgatc acaccaatac ccagcgtacg acgacccatc gctccacgtt tggcggccgg
    17701 gatcgggtaa tcctgataat ccagcagcgc gtcgagtgcg cgtactgcca gaattgccag
    17761 ctcttccagt tcatccaggc tgttaattgc gcccaggttg aaagcagaca gcgtacacag
    17821 cgcgatttca ccgttctcgt cgttgacgtc gttcagtggt ttggtcggta gggcgatttc
    17881 caggcacagg ttagactggc gcactggtgc aatagccgga tcaaacgggc tgtgggtgtt
    17941 gcagtggtct acgttctgaa tatagatacg accggtagac gcacgttcct gcatcatcag
    18001 cgagaacagc tcaacggctt tcacacgctg cttgcggatg ctgtcgtctt tctcatattt
    18061 ggtgtacagg cgttcaaact cttcctgatc ggcgaagaac gcgtcgtaca gccccggtac
    18121 gtcggacggg ctgaacaggg tgatatcttc acctttcagc agacgggtat acatcagttt
    18181 gttgatttgt accccgtagt ccatatgacg cacgcggttg ccttcaaccc cacggttgtt
    18241 tttcaacacc agcaggcttt ccacttccag atgccacatc gggtagaaca gtgttgccgc
    18301 accgccgcgc acaccgccct gagagcagga tttcaccgct gtctggaaat gtttgtaaaa
    18361 cggaatacag ccggtgtgga atgcttcgcc accgcgaatc gggctaccca acgcacgaat
    18421 acgcccggcg ttgatgccga tcccggcacg ctgggaaacg tatttaacaa tcgcgctgga
    18481 ggtggcgttg atggaatcca ggctgtcgcc gcactcgatc agtacgcagg agctgaactg
    18541 acgagtcggg gtacgcacgc cggacatgat tggcgtcggc agcgaaattt taaatgtgga
    18601 aaccgcgtcg taaaaacgct tcacgtattg caggcgcgtt tcacgcgggt agttcgagaa
    18661 caggcacgcg gcaaccagaa tataaaggaa ctgggcgctt tcgtagattt cgccggtcac
    18721 gcggttctgt accagatatt tgccttccag ctgcttaacg gcagcataag agaaggtcat
    18781 atcacggtcg tgatcgataa acgtgtccat ctgcttgaac tcttcttccg tgtagtcttc
    18841 cagcagatga ttatcgtatt tgcccatctc gaccattttc accacgtggt cgtacagcgc
    18901 aggcggctca aactggccgt aggctttttt acgcaggtgg aagatcgcca ggcgcgcggc
    18961 gagatactga taatccggcg catcacggga gatcaggtct gcggcagcct tgataatggt
    19021 ttcgtggatg tcagaggtct tgataccgtc ataaaactga atgtgggagc gcagttcgac
    19081 ctgggaaatc gaaacgttat gcagtccttc tgccgcccaa tccagaacgc gatggatttt
    19141 gtcgagattg atgcgctctg tgctaccgtc gcgctttgtc accagcagat tctgattcat
    19201 gtatgtcgta cctgtttttg gaattctttc cgcaatagtt tcatgcagac tacctgtagt
    19261 gagggaagct gcttagaaca ctatatatag ggggtatgtt tgggattcac tgcaagatag
    19321 tgtgaaaatg gccctcttgc aagtgcataa ctttgtggat aactcaggaa ggaaaaagtg
    19381 gctttcgcgc accttaggtc agacaaggtg tccgggaaag tcaatgggaa gagaaaaatt
    19441 tgttaaaaat aactgttcgg tgatttcttg a
//
LOCUS       DAZFLD010000084        19445 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.084, whole genome shotgun sequence.
ACCESSION   DAZFLD010000084 DAZFLD010000000
VERSION     DAZFLD010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19445
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.084"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..593)
                     /locus_tag="ACHKBL_002505"
     CDS             complement(<1..593)
                     /locus_tag="ACHKBL_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFO5819358.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDTPTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVAATDGPMPQTREHILLGRQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQ
                     YDFPGDDTPIVRGSALKALEGDAEWEAKILELAGFLDS"
     gene            complement(664..2778)
                     /gene="fusA"
                     /locus_tag="ACHKBL_002506"
     CDS             complement(664..2778)
                     /gene="fusA"
                     /locus_tag="ACHKBL_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HFO5819359.1"
                     /translation="MARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRMGANFLKVVNQIKT
                     RLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEW
                     HQNLIESAAEASEELMEKYLGGEELTEEEIKGALRQRVLNNEIILVTCGSAFKNKGVQ
                     AMLDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLT
                     FFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVT
                     TGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTD
                     EESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQ
                     SGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPL
                     AGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETP
                     EENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTM
                     EFLKYDEAPSNVAQAVIEARGK"
     gene            complement(2875..3345)
                     /gene="rpsG"
                     /locus_tag="ACHKBL_002507"
     CDS             complement(2875..3345)
                     /gene="rpsG"
                     /locus_tag="ACHKBL_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HFO5819360.1"
                     /translation="MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVY
                     SALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRW"
     gene            complement(3442..3816)
                     /gene="rpsL"
                     /locus_tag="ACHKBL_002508"
     CDS             complement(3442..3816)
                     /gene="rpsL"
                     /locus_tag="ACHKBL_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HFO5819361.1"
                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGAL
                     DCSGVKDRKQARSKYGVKRPKA"
     gene            complement(3942..4229)
                     /gene="tusB"
                     /locus_tag="ACHKBL_002509"
     CDS             complement(3942..4229)
                     /gene="tusB"
                     /locus_tag="ACHKBL_002509"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HFO5819362.1"
                     /translation="MLHTLHRSPWLTDFAALLRLLSEGDELLLLQDGVTAAVDGNRYL
                     ESLRNAPIKVYALNEDLIARGLAGQISNDIILIDYTDFVRLTVKHSSQMAW"
     gene            complement(4237..4596)
                     /gene="tusC"
                     /locus_tag="ACHKBL_002510"
     CDS             complement(4237..4596)
                     /gene="tusC"
                     /locus_tag="ACHKBL_002510"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HFO5819363.1"
                     /translation="MKRIAFVFSTVPHGTAAGREGLDALLATSALTDDLAVFFIADGV
                     FQLLPGQKPDAVLARDYIATFKLLGLYDIEQCWVCAASLRERGLDPQTPFVVEATPLE
                     ADALRRELANYDVILRF"
     gene            complement(4596..4982)
                     /gene="tusD"
                     /locus_tag="ACHKBL_002511"
     CDS             complement(4596..4982)
                     /gene="tusD"
                     /locus_tag="ACHKBL_002511"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HFO5819364.1"
                     /translation="MRFAIVVTGPAYGTQQASSAFQFAQALIAEGHELSSVFFYREGV
                     YNANQLTSPASDEFDLVRCWQQLNAQHGVALNICVAAALRRGIVDETEAGRLGLASSN
                     LQPGFTLSGLGALAEASLTCDRVVQF"
     gene            complement(4982..5704)
                     /gene="yheO"
                     /locus_tag="ACHKBL_002512"
     CDS             complement(4982..5704)
                     /gene="yheO"
                     /locus_tag="ACHKBL_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO5819365.1"
                     /translation="MSRSLLTNETSELDLLDQRPFDQTDFDILKSYEAVVDGLAMLIG
                     SHCEIVLHSLQDLKCSAIRIANGEHTGRKIGSPITDLALRMLHDMTGADSSVSKCYFT
                     RAKSGVLMKSLTIAIRNREQRVIGLLCINMNLDVPFSQIMSTFVPPETPDVGSSVNFA
                     SSVEDLVTQTLEFTIEEVNADRNVSNNAKNRQIVLNLYEKGIFDIKDAINQVADRLNI
                     SKHTVYLYIRQFKSGDFQGQDK"
     gene            complement(5871..6683)
                     /gene="fkpA"
                     /locus_tag="ACHKBL_002513"
     CDS             complement(5871..6683)
                     /gene="fkpA"
                     /locus_tag="ACHKBL_002513"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFO5819366.1"
                     /translation="MKSLFKVTLLATTMAVALHAPITFAAEAAKPATTADSKAAFKND
                     DQKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAFADKSKLSDQEIEQTL
                     QAFEARVKSSAQAKMEKDAADNEAKGKEYREKFAKEKGVKTSSTGLVYQVVEAGKGEA
                     PKDSDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGLKNIKKGGKIKLV
                     IPPELAYGKAGVPGIPPNSTLVFDVELLDVKPAPKADAKPEADAKAADSAKK"
     gene            6904..7122
                     /gene="slyX"
                     /locus_tag="ACHKBL_002514"
     CDS             6904..7122
                     /gene="slyX"
                     /locus_tag="ACHKBL_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HFO5819367.1"
                     /translation="MQDLSLEARLAELESRLAFQEITIEELNVTVTAHEMEMAKLRDH
                     LRLLTEKLKASQPSNIASQAEETPPPHY"
     gene            complement(7171..7761)
                     /gene="slyD"
                     /locus_tag="ACHKBL_002515"
     CDS             complement(7171..7761)
                     /gene="slyD"
                     /locus_tag="ACHKBL_002515"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFO5819368.1"
                     /translation="MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISG
                     LETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETDQ
                     GPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHH
                     DHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH"
     gene            complement(7856..8056)
                     /gene="yheV"
                     /locus_tag="ACHKBL_002516"
     CDS             complement(7856..8056)
                     /gene="yheV"
                     /locus_tag="ACHKBL_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative zinc ribbon protein"
                     /protein_id="HFO5819369.1"
                     /translation="MAIRKRFIAGAKCPACQAQDSMAMWRENNIDIVECVKCGHQMRE
                     ADKEARDHVRKDEQVIGIFHPD"
     gene            complement(8066..9871)
                     /gene="kefB"
                     /locus_tag="ACHKBL_002517"
     CDS             complement(8066..9871)
                     /gene="kefB"
                     /locus_tag="ACHKBL_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015503 - glutathione-regulated potassium
                     exporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefB"
                     /protein_id="HFO5819370.1"
                     /translation="MEGSDFLLAGVLFLFAAVAAVPLASRLGIGAVLGYLLAGIAIGP
                     WGLGFISDVDEILHFSELGVVFLMFIIGLELNPSKLWQLRRSIFGVGAAQVLLSAALL
                     AGLLMLTDFAWQAAVVGGIGLAMSSTAMALQLMREKGMNRSESGQLGFSVLLFQDLAV
                     IPALALVPLLAGSADEHFDWMKIGMKVLAFVGMLIGGRYLLRPVFRFIAASGVREVFT
                     AATLLLVLGSALFMDALGLSMALGTFIAGVLLAESEYRHELETAIDPFKGLLLGLFFI
                     SVGMSLNLGVLYTHLLWVVISVVVLVTVKILVLYLLARLYGVRSSERMQFAGVLSQGG
                     EFAFVLFSTASSQRLFQGDQMALLLVTVTLSMMTTPLLMKLVDKWLSRQFNGPEEEDE
                     KPWVNDDKPQVIVVGFGRFGQVIGRLLMANKMRITVLERDISAVNLMRKYGYKVYYGD
                     ATQVDLLRSAGAEAAESIVITCNEPEDTMKLVEICQQHFPHLHILARARGRVEAHELL
                     QAGVTQFSRETFSSALELGRKTLVTLGMHPHQAQRAQLHFRRLDMRMLRELIPMHADT
                     VQISRAREARRELEEIFQREMQQERRQLDGWDEFE"
     gene            complement(9871..10422)
                     /gene="kefG"
                     /locus_tag="ACHKBL_002518"
     CDS             complement(9871..10422)
                     /gene="kefG"
                     /locus_tag="ACHKBL_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312229.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     ancillary protein KefG"
                     /protein_id="HFO5819371.1"
                     /translation="MSQPAKVLLLYAHPESQDSVANRVLLKPATQLSNVTVHDLYAHY
                     PDFFIDIPREQALLREHEVIVFQHPLYTYSCPALLKEWLDRVLSRGFASGPGGNQLAG
                     KYWRSVITTGEPESAYRYDALNRYPMSDVLRPFELAAGMCRMHWLSPIIIYWARRQSA
                     QELASHARAYGDWLANPLSPGGR"
     gene            10553..12466
                     /gene="yheS"
                     /locus_tag="ACHKBL_002519"
     CDS             10553..12466
                     /gene="yheS"
                     /locus_tag="ACHKBL_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO5819372.1"
                     /translation="MIVFSSLQIRRGVRVLLDNATATINPGQKVGLVGKNGCGKSTLL
                     ALLKNEISADGGSYTFPGSWQLAWVNQETPALPQAALEYVIDGDREYRQLEAQLHDAN
                     ERNDGHAIATIHGKLDAIDAWSIRSRAASLLHGLGFSNEQLERPVSDFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLEKWLKSYQGTLILISHDRDFLDPIVDKIIH
                     IEQQSMFEYTGNYSSFEVQRATRLAQQQAMYESQQERVAHLQSYIDRFRAKATKAKQA
                     QSRIKMLERMELIAPAHVDNPFRFSFRAPESLPNPLLKMEKVSAGYGDRIILDSIKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELAPVSGEIGLAKGIKLGYFAQHQLEYLRADE
                     SPIQHLARLAPQELEQKLRDYLGGFGFQGDKVTEETRRFSGGEKARLVLALIVWQRPN
                     LLLLDEPTNHLDLDMRQALTEALIEFEGALVVVSHDRHLLRSTTDDLYLVHDRKVEPF
                     DGDLEDYQQWLSDVQKQENQTDEAPKENANSAQARKDQKRREAELRAQTQPLRKEIAR
                     LEKEMEKLNAQLAQAEEKLGDSELYDQSRKAELTACLQQQASAKSGLEECEMAWLEAQ
                     EQLEQMLLEGQSN"
     gene            12466..13488
                     /gene="yheT"
                     /locus_tag="ACHKBL_002520"
     CDS             12466..13488
                     /gene="yheT"
                     /locus_tag="ACHKBL_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFO5819373.1"
                     /translation="MAQITTTDANEFSSSAEFTPMRGFSNCHLQTMLPRLFRRKVKFT
                     PHWQRLELPDGDFVDLAWSEDPAQARHKPRLVVFHGLEGSLNSPYAHGLVEAAQKRGW
                     LGVVMHFRGCSGEPNRMHRIYHSGETEDASWFLRWLQREFGHAPTAAVGYSLGGNMLA
                     CLLAKEGNNLPIDAAVIVSAPFMLEACSYHMEKGFSRVYQRYLLNLLKANAARKLAAY
                     PGTLPINLAQLKSVRRIREFDDLITARIHGYADAIDYYRQCSAMPMLNRIAKPTLIIH
                     AKDDPFMDHQVIPKPESLPPQVEYQLTEHGGHVGFIGGTLLHPQMWLESRIPDWLTTY
                     LEAKSC"
     gene            13482..13700
                     /gene="yheU"
                     /locus_tag="ACHKBL_002521"
     CDS             13482..13700
                     /gene="yheU"
                     /locus_tag="ACHKBL_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HFO5819374.1"
                     /translation="MLIPWQDLSPETLENLIESFVLREGTDYGEHERTLEQKVADVKR
                     QLQCGEAVLVWSELHETVNIMPRSQFRE"
     gene            13754..14623
                     /gene="prkB"
                     /locus_tag="ACHKBL_002522"
     CDS             13754..14623
                     /gene="prkB"
                     /locus_tag="ACHKBL_002522"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008974 - phosphoribulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HFO5819375.1"
                     /translation="MSAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSF
                     HRYTRPEMDMAIRKARDAGRHISYFGPEANDFGLLEQTFIEYGQSGKGKSRKYLHTYD
                     EAVPWNQVPGTFTPWQPLPEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLE
                     WIQKLIRDTSERGHSREAVMDSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFA
                     AKGIPSLDESFVVIHFRNLEGIDFPWLLAMLQGSFISHINTLVVPGGKMGLAMELIML
                     PLVQRLMEGKKIE"
     gene            complement(14678..15082)
                     /gene="yhfA"
                     /locus_tag="ACHKBL_002523"
     CDS             complement(14678..15082)
                     /gene="yhfA"
                     /locus_tag="ACHKBL_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HFO5819376.1"
                     /translation="MQARVKWVEGLTFLGESASGHQILMDGNSGDKAPSPMEMVLMAA
                     GGCSAIDVVSILQKGRQDVVDCEVKLTSERREEAPRLFTHINLHFIVTGRDLKDAAVA
                     RAVDLSAEKYCSVALMLEKAVNITHSYEVVAA"
     gene            15384..16016
                     /gene="crp"
                     /locus_tag="ACHKBL_002524"
     CDS             15384..16016
                     /gene="crp"
                     /locus_tag="ACHKBL_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HFO5819377.1"
                     /translation="MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIV
                     KGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEGQERSAWVRAKTACEVAEISYKK
                     FRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
                     PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGTR"
     gene            16067..18157
                     /gene="yhfK"
                     /locus_tag="ACHKBL_002525"
     CDS             16067..18157
                     /gene="yhfK"
                     /locus_tag="ACHKBL_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312236.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccS/YhfK family putative transporter"
                     /protein_id="HFO5819378.1"
                     /translation="MWRRLIYHPDINYALRQTLVLCLPVAVGLMLGELRFGLLFSLVP
                     ACCNIAGLDTPHKRFFKRLIIGASLFATCSLLTQVLLAKDVPLPFLLTGLTLVLGVTA
                     ELGPLHAKLLPASLLAAIFTLSLAGYMPVWEPLLIYALGTLWYGLFNWFWFWIWREQP
                     LRESLSLLYRELADYCEAKYSLLTQHTDPEKALPPLLVRQQKAVDLITQCYQQMHMLS
                     AQNNTDYKRMLRIFQEALDLQEHISVSLHQPEEVQKLVERSHAEEVIRWNAQTVAARL
                     RVLADDILYHRLPTRFTMEKQIGALEKIARQHPDNPVGQFCYWHFSRIARVLRTQKPL
                     YARDLLADKQRRMPLLPALKSYLSLKSPALRNAGRLSVMLSVASLMGTALHLPKSYWI
                     LMTVLLVTQNGYGATRLRIVNRSVGTVVGLIIAGVALHFKIPEGYTLTLMLITTLASY
                     LILRKNYGWATVGFTITAVYTLQLLWLNGEQYILPRLIDTIIGCLIAFGGTVWLWPQW
                     QSGLLRKNAHDALEAYQDAIRLILSEDPQPTPLAWQRMRVNQAHNTLYNSLNQAMQEP
                     AFNSHYLADMKLWVTHSQFIVEHINAMTTLAREHRALPPELAQEYLQSCEIAIQRCQQ
                     RLEYDEPGSSGDANIMDAPEMQPHEGAAGTLEQHLQRVIGHLNTMHTISSMAWRQRPH
                     HGIWLSRKLRDSKA"
     gene            complement(18224..19444)
                     /gene="argD"
                     /locus_tag="ACHKBL_002526"
                     /gene_synonym="dapC"
     CDS             complement(18224..19444)
                     /gene="argD"
                     /locus_tag="ACHKBL_002526"
                     /gene_synonym="dapC"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     acetylornithine/succinyldiaminopimelate transaminase"
                     /protein_id="HFO5819379.1"
                     /translation="MAIEQTAITRATFDEVILPIYAPAEFIPVKGQGSRIWDQQGKEY
                     VDFAGGIAVTALGHCHPALVNALKTQGETLWHISNVFTNEPALRLGRKLIEATFAERV
                     VFMNSGTEANETAFKLARHYACVRHSPFKTKIIAFHNAFHGRSLFTVSVGGQPKYSDG
                     FGPKPADIIHVPFNDLHAVKAVMDDHTCAVVVEPIQGEGGVTAATPEFLQGLRELCDQ
                     HQALLVFDEVQCGMGRTGDLFAYMHYGVTPDILTSAKALGGGFPISAMLTTAEIASAF
                     HPGSHGSTYGGNPLACAVAGAAFDIINTPEVLEGIQAKRQRFVDHLQKIDQQYDVFSD
                     IRGMGLLIGAELKPQYKGRARDFLYAGAEEGVMVLNAGPDVMRFAPSLVVEDADIDEG
                     MHRFAHAVAKVIGA"
BASE COUNT         4763 a         5329 c         4837 g         4516 t
ORIGIN      
        1 gaatccagga agccagccag ttccaggatt ttcgcttccc actctgcgtc gccttccagc
       61 gctttcagag cagaaccacg aacgatcgga gtgtcgtcgc ccgggaagtc gtactgagac
      121 agaagttcac gaacttccat ttcaaccagt tccagcagct cttcgtcatc aaccatgtca
      181 catttgttca ggaacacgat gatgtacgga acgcctacct gacgacccag caggatgtgc
      241 tcacgagtct gcggcatcgg gccgtcagtc gcagcaacta ccaggatcgc gccgtccatc
      301 tgagcagcac cggtgatcat gtttttaaca tagtcggcgt gccccgggca gtctacgtgt
      361 gcgtagtgac gggtcggggt gtcgtattca acgtgagaag tgttgatggt gataccacga
      421 gctttttctt ccggcgcgtt atcgatctgg tcgaatgcac gagcagcacc gccgtaggtt
      481 ttagccagta cggtggtgat tgcagcggtc agagtagttt taccgtggtc aacgtggccg
      541 atagtaccaa cgttaacgtg cggttttgta cgttcaaatt tttctttaga cacggctata
      601 ttccttacta tagtgctctc cccttcagga gagagcacgg gactttggta ttaaccctta
      661 ggcttattta ccacgggctt caattacggc ctgagcaacg ttactcggcg cttcatcata
      721 cttcaggaat tccatggtgt atgatgcacg acctttggtc agagaacgca gctgagttgc
      781 atatccgaac atttcagaca gcggtacttc agcgtggatc ttaacgccag taacttcaga
      841 ttcctgacct ttgagcatac cacgacgacg gctcaagtca ccgataacgt caccggtgtt
      901 ctcttccgga gtttctactt caaccttcat gatcggctca agcagaactg gtttcgcttt
      961 cttaaagcct tctttaaagg cgatagaagc agccagttta aacgccagtt cagaggagtc
     1021 aacgtcatgg taagaaccga agtgcagacg aatacccatg tctactaccg ggtagcctgc
     1081 cagcggacct gctttcagct gttcctggat acctttatca acggccggga tgtattcgcc
     1141 agggattaca ccacctttaa tgtcgttgat gaactcgtag cctttcgggt ttgaacccgg
     1201 ctccagcggg tacatgtcga taacaacatg accatactga ccacgaccac cagactgttt
     1261 cgcgtgttta ccttcaacat cggtaacttt ctggcggata gtttcacggt aagcaacctg
     1321 cggtttacct acgttcgctt caacgttgaa ttcacgcttc atacggtcaa cgatgatgtc
     1381 gaggtgcagt tcgcccatac ccgcgatgat ggtctggtta gattcttcgt cagtccatac
     1441 acggaaagac gggtcttctt tagccagacg gcccagagcc agacccattt tttcctggtc
     1501 agctttggtt ttcggttcaa ctgcgatgga gattaccggc tcagggaatt ccatacgttc
     1561 cagaatgatc ggcgcatccg ggtcacacag ggtgtcacca gtggttacgt ctttcagacc
     1621 gatagcagca gcgatgtcgc ccgcgcgaac ttctttgatc tcttcacgtt tgttagcgtg
     1681 catctgaacg atacgaccga aacgctcacg tgcagctttc acggagttca gtacggtatc
     1741 accagagtta accacaccgg agtaaacacg gaagaaggtc aggttaccaa caaacgggtc
     1801 ggtagcgatt ttgaacgcca gtgcagagaa cggctcgtca tcacttgcgt gacgttcagc
     1861 cggagtgtct ttaccgtcgt ccaggatacc gttgatcgca ggtacgtcaa ccggggatgg
     1921 caggtaatcg attaccgcat ccagcatcgc ctgaacacct ttgttcttga acgcagaacc
     1981 acaggttacc aggatgattt cgttgttcag aacgcgctga cgcagagcac ctttaatctc
     2041 ttcttcagtc agttcttcac cacccaggta tttttccatc agctcttcag aagcttcagc
     2101 tgcggattcg atcaggttct ggtgccattc gttagccagt tcaaccatgt ctgccgggat
     2161 atcttcgtat tcgaaggtta cgccctggtc agcgtcgttc cagttgatag ctttcatttt
     2221 caccaggtca acaacaccgg tgaaatgttc ttcagcacca atcgccagct gcagcggaac
     2281 cgggttcgcg cccagacggg ttttgatctg gttaacaact ttcaggaagt tcgcacccat
     2341 gcggtccatt ttgttaacga acgcaatgcg cggaacttta tatttgtttg cctgacgcca
     2401 tacggtttca gactgcggct gaacaccacc aactgcgcag taaaccatta ccgcaccatc
     2461 gagaacacgc atggaacgtt ctacttcgat tgtgaagtca acgtgccccg gggtgtcgat
     2521 gatgttgatg cgatgcggct catactgctt agccatacca gaccagaatg cagtagtcgc
     2581 agcggaagtg atggtaatac cacgttcctg ctcctgctcc atccagtcca tggttgcagc
     2641 gccgtcatga acttcaccga ttttatggtt tacaccggtg tagaacagaa tacgttcggt
     2701 agtagtggtt ttaccggcgt cgatgtgcgc actgataccg atgttacggt agcgtgcgat
     2761 gggtgttgta cgagccattt gtttcctcgt ttatctttta ggcgttcaat ttaagtagcc
     2821 caaagcgggc tgcttactgg aagcgcccgc ctggtgacta aaactccgaa gggattacca
     2881 acggtagtgt gcgaacgcct tgttggcttc ggccatacgg tgaacgtctt cacgtttctt
     2941 aactgcagta cctttgtttt ctgcagcatc agaaagttcg ttcgccaggc gcagagccat
     3001 ggatttatca ccgcgtttac gagcagcttc aacgatccaa cgcattgcca gagcattacg
     3061 acgaaccgga cggacttcaa ctggtacctg ataagtagaa ccaccaacgc ggcgagactt
     3121 aacttctaca gtcgggcgca cgttttcgag agctacttcg aatgcttcca gttcagattt
     3181 accagagcgc tgagccaggg tctccagcgc gctgtatacg atagattcag cagtagattt
     3241 tttaccatct accatcagga tatttacaaa tttagccagc agttctgatc cgaacttcgg
     3301 atccggcaga attttacgct gaccaatgac gcgacgacgt ggcatggaaa tactccgttg
     3361 ttaattcagg attgtccaaa actctacgag tttagtttga catttaagtt aaaacgtttg
     3421 gccttactta acggagaacc attaagcctt aggacgcttc acgccatact tggaacgagc
     3481 ctgcttacgg tctttaacgc cggagcagtc aagcgcacca cgtacggtgt ggtaacgaac
     3541 acccgggagg tctttaacac gaccgccacg gatcaggatc acggagtgct cctgcaggtt
     3601 gtgaccttca ccaccgatgt aggaagtcac ttcgaaaccg ttagtcagac gaacacggca
     3661 tactttacgc agcgcggagt tcggtttttt aggagtggta gtatatacac gagtacatac
     3721 gccacgtttt tgcgggcatg cttccagcgc aggcacgttg cttttcgcaa ctttgcgagc
     3781 acgtggtttg cgtaccagct ggttaactgt tgccattaaa tagctcctgg ttttagcttt
     3841 tgcttcgtaa acacgtaata aaacgtcctc acacaatatg aggacgccga attttagggc
     3901 gatgccgaaa aggtgtcaag aaatatacaa cgatcccgcc atcaccaggc catctggctg
     3961 gagtgcttaa ccgtaagtct gacgaaatca gtatagtcaa tgagaatgat atcgttcgaa
     4021 atttgaccag ccaaaccgcg ggcaataagg tcttcgttca gggcatagac cttaatgggg
     4081 gcattacgca gactttcaag gtagcggtta ccgtcaactg cggcagttac accatcttgc
     4141 aataatagca gttcgtcgcc ctcacttagc aggcgcagca gcgcagcaaa atccgtcagc
     4201 cagggtgaac gatgtaatgt gtgcagcata aacgcctcaa aacctcaaaa taacatcgta
     4261 gttggcgagt tcgcggcgta aggcatctgc ttcgagcggc gtggcttcga caacaaaggg
     4321 tgtctgcgga tctaacccgc gttcgcgcag tgaagccgca caaacccagc actgttcaat
     4381 gtcgtacaga cccaacaatt taaaagtggc gatgtaatca cgcgccagca ctgcatcggg
     4441 cttttgtcct ggcagcagct gaaaaacgcc atcagcaata aagaagacgg ccagatcgtc
     4501 agttaatgcg gaagttgcca gtaaagcatc taaaccttcc cgaccagcag ctgtaccatg
     4561 aggcacagta gaaaaaacaa acgcaattcg tttcatcaga actgtaccac cctgtcacag
     4621 gtcagcgagg cttccgccag cgcgccaagt ccgcttaagg taaatcccgg ctgaaggttt
     4681 gacgaagcca gccccagtct tccggcctcc gtttcatcga cgataccgcg gcgtaacgct
     4741 gccgctacgc agatattcag cgccacacca tgttgcgcat tcaactgttg ccagcagcgc
     4801 acaaggtcaa attcatcgct tgccggtgag gtcaattggt tagcgttata gaccccttcc
     4861 cgataaaaaa agacgctgct taactcatgg ccttctgcaa tcagcgcctg cgcaaactga
     4921 aaagcactac tcgcctgttg cgtaccgtat gctggcccgg tcaccacgat ggcaaaacgc
     4981 attacttatc ttgcccctgg aaatcaccgc tcttgaactg gcggatgtag agatagacag
     5041 tgtgtttgga gatgttcagg cggtcagcaa cctggttgat cgcatcttta atatcgaaga
     5101 tccctttctc gtagagattc agcacgatct gacgattttt ggcgttatta gaaacattgc
     5161 gatcggcatt cacttcttcg atggtgaact ccagcgtttg ggtaaccaga tcttcaacag
     5221 aagaggcaaa gttgacgctt gaaccgacat ccggggtttc tggcggcaca aaggtgctca
     5281 taatctgcga gaagggaaca tcaagattca tattgatgca cagcaaacca attacacgct
     5341 gttcgcggtt acgaatcgcg atagtcaggg acttcattaa tacgccgctt ttggcgcgag
     5401 taaagtagca tttagaaacg ctgctatccg ctcccgtcat atcgtgcagc atacgtagcg
     5461 ccaggtcggt aattggcgaa ccaatcttcc ggcctgtatg ttcaccgtta gcaatgcgaa
     5521 tggctgaaca ttttagatcc tgcaaagagt gcaaaacgat ttcacagtgg gagccaataa
     5581 gcatcgctaa cccgtccacc accgcttcgt aggatttcag aatatcaaaa tcggtctggt
     5641 cgaaaggacg ttgatccagt aaatccaact cactggtttc gttggttaaa agcgacctgg
     5701 acatgaaaaa aaacactcct tttcaggagc ctgtcgttaa cttttcaggg caggctcatt
     5761 aatgatgcgg gtaactaaat taatacagcg gaggttccgc tttccagcac taattatatc
     5821 cggcctgtaa taaaaaaacc gccgcctggt caggcggcgg ttcttaatgc ttatttttta
     5881 gcagaatctg cggctttcgc atcagcttcc ggctttgcat cagccttcgg cgctggtttc
     5941 acatccagca gctctacgtc aaacaccagg gtagaattcg gtgggatccc cggaacaccc
     6001 gctttgccgt aagccagttc tggtggaata accagtttga tcttaccgcc tttcttgatg
     6061 ttcttcagac cttctgtcca acccggaata acaccgtcca gacggaaaga gagcggttca
     6121 ccacgggtgt aagagttgtc gaactcttta ccgtcgatca gcgtaccttt gtagttcacg
     6181 acaacagtat cgctgtcttt cggtgcttcg cctttaccgg cttcgactac ctgataaacc
     6241 agaccagttg aagaggtttt cacacctttc tctttggcaa atttctcgcg gtactcttta
     6301 cctttcgctt cgttatctgc cgcgtctttt tccatcttcg cctgagcaga agacttcacg
     6361 cgagcttcga atgcttgcag tgtctgttcg atctcttggt cggagagttt gctcttatca
     6421 gcaaatgcat cctgaacacc agcgatcagc tgatctttat ccagtttgat gcccagtttt
     6481 tcttgttctt taagagaatt ttccatgtaa cgacccagcg aagcacccag tgcataagct
     6541 gatttctgat cgtcattttt gaacgctgct ttgctgtcag cagttgtagc aggttttgca
     6601 gcttcagcag caaaagtgat cggtgcatgc agggcaacgg ccattgtggt cgccagcagc
     6661 gttactttaa acagtgattt catccatatc tccaggatcg gggcatctca ccccagggtt
     6721 aactattatc agaagggtac tataaagcgt tgtcgaacaa atctacatac agacacgccc
     6781 tattatcatc tattttcaga ctctttttgt ttaaattagt ttcgatgacc gcgaaatgag
     6841 tgctgtctcg ggcagcaaag ttaagtagaa tccgcggcga ccattcgaca aaagaggtga
     6901 atcatgcagg atttatcatt ggaagcacgc ctggcagagc tggagagccg actggctttt
     6961 caggaaatca ccattgaaga actgaacgtc acggtgaccg ctcatgaaat ggagatggcg
     7021 aaactgcgcg atcatctgcg tctgctgacc gagaagctaa aagccagcca gccgtcgaac
     7081 atcgcgtcgc aggctgaaga aacgccaccg ccacattatt gaggcgtaaa aaaagcgggg
     7141 attccccgct tttttgtcac tttttcggta ttagtggcaa ccgcaaccgc cgttaccttt
     7201 accgccgcag cagccttcgc caccgtgttc atgaccgtga tcgtggccat gaccaccgca
     7261 gcaaccgtcg tggtcgtgat cgtggtgatg atcgtgcgca ccgtgaacgt ggccatgagc
     7321 cagttcttct tcagtcgctt cgcgaatcgc cacaacttca acgttgaatt tcaggttctg
     7381 accggccagc atgtggttac catcaaccac gacgtgatcg tcttcaaccg cagtgatttc
     7441 aaccggtacc ggaccctggt cggtttcagc caggaaacgc ataccaacct gcagttcatc
     7501 aacgcccata aatacgtctt taggaacgcg ttgcaccagg ttttcgtcgt actgaccgta
     7561 agcgtcgttc gcgccaacag cgacatcaaa tttgtcgcca acttcgtgac cttccagcgc
     7621 cgtttccagg ccagagatca gggaaccgtg accatgcagg tagtccagcg gcgcactcac
     7681 cggagactca tcaaccaaca caccgtcttc tgtacgtacc tgataggcca ggctgaccac
     7741 caggtctttt gctactttca tgatatctcc tgagcatggg aagaatagtg gcgcagattg
     7801 tagcggaatt ctgcagccgt gtactcacta gcttaaaaaa actcggcgca tatcgctagt
     7861 ccggatgaaa aatcccgatc acttgctcat ctttgcgaac gtgatcgcgg gcttctttgt
     7921 ccgcttctcg catctgatgt ccgcacttaa cacattcaac aatatcaata ttattttcgc
     7981 gccacatcgc cattgaatcc tgcgcctgac aggccgggca ttttgccccc gcaataaaac
     8041 gttttcggat tgccatcttt accctctact caaattcatc ccagccgtcc agctggcgtc
     8101 gttcttgttg catttcacgc tggaaaatct cttccagttc gcgtcgggct tccctggcgc
     8161 gagaaatttg tacggtgtcg gcatgcattg ggatgagctc ccgcaacatc cgcatatcca
     8221 ggcggcgaaa atgcagttgc gcccgctgcg cctgatgtgg atgcatgcct agcgtgacca
     8281 gcgtcttgcg ccccagctct aacgcactgg agaatgtctc acgagaaaac tgcgtcaccc
     8341 ctgcctgtaa taactcatgc gcctccacac gtccgcgcgc tcgcgcaaga atatgtaaat
     8401 gcggaaagtg ctgctggcat atttccacca gtttcatggt gtcttccggc tcattacagg
     8461 taatgacgat agactcagcg gcctctgcac ccgcagaacg taaaagatct acctgcgtgg
     8521 cgtcgccgta ataaactttg tagccatatt tgcgcatcag gttaacggcg ctgatatccc
     8581 gctccagcac agtaatgcgc attttatttg ccatcagcaa acgaccaatc acctgaccaa
     8641 aacgcccgaa gcccacgaca atgacctgcg gtttatcatc gttgacccac ggtttttcgt
     8701 cttcttcttc cggtccgtta aactggcggg atagccattt atccaccagc ttcatcagca
     8761 acggcgtggt catcatggaa agcgtcaccg tcaccagcaa cagcgccatc tggtcgccct
     8821 gaaacaaccg ttgcgaagaa gcggtagaaa agagcacaaa ggcaaactca ccgccctgac
     8881 tcaacacgcc agcaaactgc atccgctctg agctacgcac gccatataat cgcgccagca
     8941 gatacagcac gagaattttc accgtcacca gcacaaccac gcttatcact acccacaaca
     9001 gatgggtata aagcaccccg agattgagcg acatgccgac agagataaag aacaaaccga
     9061 gcagcaagcc tttgaaggga tcgatagctg tttccagttc gtggcgatat tcgctttctg
     9121 ccagcaacac gcccgcaata aacgtaccga gcgccatcga cagccccagc gcatccataa
     9181 acaatgcgga acccaacacc agcagcagcg tcgcggcggt gaacacttcc cgcacgccag
     9241 aagctgcaat aaaacggaat accggacgca gtaaatagcg cccgccaatc agcatgccga
     9301 caaacgccag caccttcatg ccgatcttca tccagtcgaa atgttcgtct gccgaacccg
     9361 ccaacaacgg cactaacgcc agcgccggga tcactgccag atcctgaaac agcagaaccg
     9421 aaaaacctag ctgcccggat tcgctgcgat tcatcccttt ctcacgcatc agttgcaacg
     9481 ccattgcggt tgaggacatc gcaaggccaa taccaccgac caccgccgcc tgccaggcga
     9541 aatccgtcag catcaataat cccgccagca acgccgcgct taacagcacc tgtgccgcac
     9601 ctacgccaaa aatcgaacgc cgcaattgcc aaagcttgga gggattcaac tccaggccga
     9661 tgataaacat caggaacacc acgccgagtt ccgaaaagtg gaggatctca tcgacgtcgc
     9721 taataaaacc cagcccccac gggccaattg caatccctgc cagcaaatat cccaacacag
     9781 cgccaatacc cagccgcgat gccagcggca ccgcagccac cgccgcgaag agaaacaaca
     9841 ctcctgcgag taaaaaatcg gaaccttcca tcagcggcct cctggagaca gcggatttgc
     9901 cagccagtca ccgtaggctc tggcgtgact cgccagctcc tgtgcgcttt gccgtctcgc
     9961 ccagtaaata atgatgggac tcaaccaatg catccggcac atgcccgccg ctagttcaaa
    10021 ggggcgcaga acatcgctca tcgggtagcg attcaaggcg tcataacggt aagcactttc
    10081 cggctcgccg gtggtaatca cgctacgcca gtactttccc gccagttggt ttcctcccgg
    10141 cccgctggca aaaccacggc ttaatacccg gtccagccac tctttcagta gcgccgggca
    10201 gctataggta taaagaggat gctgaaagac aatcacctcg tgctcgcgca gtaatgcctg
    10261 ctcacggggg atatcaataa aaaaatcggg atagtgcgcg taaaggtcgt gcacggtaac
    10321 attgctgagc tgcgtggccg gtttaagcag tacccggttt gccaccgagt cctgagattc
    10381 cggatgggca tacagcagca aaactttcgc tggctgagac atcatccccc tcccggtatg
    10441 gtttttgtgt atagtcgctg ttttgggcta ccattgcgcc cggtgcggca gctcgcccat
    10501 acattacatt atcataatga taagttaaca tagtctgaac atacggcgcc ttatgattgt
    10561 tttttcctcg ttacaaattc gtcgcggcgt gcgcgtcctg ctggataatg ccaccgccac
    10621 catcaacccc gggcagaaag tcggcctggt gggtaaaaac ggctgtggta aatcgaccct
    10681 gctggcattg ctgaaaaatg aaatcagcgc cgacggcggc agctacacct ttccgggaag
    10741 ctggcaactg gcgtgggtga atcaggaaac gccggcgtta ccgcaagcgg cgctggaata
    10801 tgtcattgac ggcgaccgtg aatatcgtca actggaagcg cagctacacg acgccaacga
    10861 acgtaacgac gggcacgcca tcgccactat tcatggcaag ctggatgcta tcgacgcatg
    10921 gagtattcgc tcccgtgccg ccagcctgct gcacggcctc ggtttcagca atgaacaact
    10981 ggagcgcccg gtaagtgatt tctccggtgg ctggcgtatg cgtcttaacc ttgcccaggc
    11041 gctgatttgc cgttcagact tgctgctgct cgatgaaccg actaaccacc tcgatctcga
    11101 tgccgttatc tggctggaaa aatggctgaa gagctatcag ggcacgctga tcctgatctc
    11161 tcacgaccgc gacttcctcg atccgatcgt tgataaaatt attcatatcg aacaacaaag
    11221 catgttcgag tacaccggca actacagttc gtttgaagta cagcgcgcca cccgtctggc
    11281 gcagcaacaa gcgatgtatg aaagccagca ggaacgcgtg gcgcatctgc aaagttatat
    11341 cgaccgtttc cgtgccaaag ccaccaaagc gaagcaggcc cagagccgta ttaagatgct
    11401 ggagcgtatg gagctgattg ccccggcgca cgttgacaac ccgtttcgtt ttagcttccg
    11461 cgcgccggaa agcctgccta atccgttatt aaagatggaa aaagtcagcg caggctatgg
    11521 tgatcgcatt attctcgact cgattaaact gaatctggtc cccggctcgc gcattggtct
    11581 gctaggccgc aacggcgcgg gtaaatcgac attaatcaaa ctgttagccg gcgagcttgc
    11641 gccagtcagc ggtgaaattg gtctggcgaa agggatcaag ctcggctact tcgcccagca
    11701 tcaactggaa tacctgcgcg ccgacgaatc gcctattcaa catctggcac gtttagcgcc
    11761 gcaggaactg gagcaaaaac tgcgtgacta cctcggcggc tttggtttcc agggcgataa
    11821 agtgacggaa gaaacgcgcc gcttctccgg tggggaaaaa gcccgcctgg tgctggcatt
    11881 aattgtctgg cagcgtccga atctgctgct gctcgacgaa ccgactaacc accttgacct
    11941 cgacatgcgt caggcactga ctgaagcatt aatcgagttc gaaggcgcgc tggttgtcgt
    12001 ttcgcacgac cgtcatttgc tgcgttccac cactgacgat ctctacctgg ttcacgatcg
    12061 taaagtcgaa ccgttcgacg gcgatctgga agattatcaa cagtggttga gcgacgtaca
    12121 aaagcaggaa aaccagaccg acgaagcgcc aaaagagaac gcaaacagcg cccaggcacg
    12181 taaagatcag aagcgtcggg aagcggagct gcgtgcgcaa acccaaccgc tgcgtaaaga
    12241 gatcgcccgt ctggaaaaag agatggaaaa gctgaacgca cagctggcgc aggcggaaga
    12301 gaaactcggc gacagcgaac tgtacgatca aagccgtaaa gcggagttga ccgcctgcct
    12361 gcaacagcaa gccagcgcca aatccggcct ggaagagtgc gaaatggcgt ggctggaagc
    12421 ccaggagcag cttgagcaga tgctgctgga aggccaaagc aactgatggc gcagataacg
    12481 acgaccgatg ccaatgaatt cagcagcagt gctgaattca cccctatgcg cggctttagc
    12541 aattgtcatc tacaaaccat gctgccgcgt ctgtttcgtc gcaaggtgaa attcaccccg
    12601 cactggcagc gactggagtt gcccgacggc gattttgtcg atctcgcgtg gagtgaagat
    12661 cctgcacagg cgcggcataa accgcgttta gtcgtgtttc acgggctcga aggcagtctc
    12721 aatagccctt acgcccacgg tctggttgag gcggcgcaaa agcgcggctg gctgggcgtg
    12781 gtgatgcatt ttcgcggatg cagcggtgaa ccaaaccgta tgcaccgcat ttaccattcg
    12841 ggtgaaaccg aagacgccag ctggttttta cgctggctgc agcgcgaatt tggacatgcg
    12901 ccaacggctg ccgtcggcta ttcgctcggc ggtaatatgc tcgcctgttt gctggcaaaa
    12961 gaaggtaata acctcccgat tgatgcagcg gtaattgtct ccgcgccatt tatgctggaa
    13021 gcctgtagtt atcatatgga aaagggcttt tcccgcgttt atcagcgtta cttgctgaac
    13081 ctgttaaaag ccaatgccgc gcgcaagctg gcagcctacc ccggaacact gccgattaat
    13141 ctcgcgcagt taaaatcggt acgtcgcatc cgtgaatttg acgatctaat caccgccaga
    13201 attcacggct acgctgacgc tatcgactat tatcgtcagt gtagcgccat gccgatgctc
    13261 aatcggatcg ccaaaccgac gctgattatt cacgccaaag acgatccgtt tatggatcat
    13321 caggtgatcc cgaaaccgga aagtctcccc ccgcaggtgg agtatcaact gactgaacat
    13381 ggcggtcatg ttggctttat tggcggtaca ttacttcatc cgcaaatgtg gctggagtca
    13441 cgcattcctg actggttaac aacgtatctg gaggcgaaat catgttgatt ccgtggcaag
    13501 acctctcccc cgaaacgctg gaaaatttaa ttgaaagctt tgtgttacgt gaaggcaccg
    13561 attatggtga acatgagcgt acacttgaac agaaagtcgc cgacgtcaaa cgccagctac
    13621 agtgcggaga agcggtgctg gtatggtcgg agctgcacga aacggtcaat atcatgccgc
    13681 gcagtcagtt tcgcgaataa cctgcaaccg tcggcggacg agataataat gatagctaag
    13741 tcatggagtt actatgtctg ccaaacatcc ggtcattgcg gtaacaggat ccagcggcgc
    13801 ggggaccacc accaccagcc tcgcgtttcg taaaatattc gcgcagttaa atctgcatgc
    13861 agccgaggtg gaaggcgaca gttttcaccg ttacacccgc ccggaaatgg acatggcgat
    13921 ccgcaaagca cgcgacgccg ggcggcatat cagctacttc ggccccgaag cgaacgactt
    13981 cggcctgctg gaacaaacct tcattgaata tggtcagagc ggcaaaggga aatctcgcaa
    14041 atatctgcat acctacgatg aagccgtacc gtggaatcag gtaccgggga cattcacccc
    14101 ctggcaacct ttaccggaac ccactgatgt actgttttat gaaggtttac acggcggcgt
    14161 agtcacgcca cagcataacg ttgcgcagca tgtggactta ctggtcggcg tggtgcctat
    14221 cgttaacctt gagtggattc aaaaactgat ccgcgacacc agcgagcgcg ggcactcacg
    14281 agaagcagtg atggactcgg tagtgcgttc aatggaagac tatatcaact acatcacacc
    14341 gcagttttcc cgcacccatc ttaacttcca gcgcgttccc accgtcgaca cttcaaaccc
    14401 gttcgcggca aaaggtatcc cgtcgctgga tgaaagcttt gtggtgatcc attttcgtaa
    14461 tctggaaggg atcgatttcc cctggctgct ggcgatgttg caaggctcat tcatttccca
    14521 catcaatacg ttagtggtac cgggcggcaa aatgggtctg gcaatggaat taattatgct
    14581 gccgctggtg caacgattga tggaaggaaa gaaaatcgag taactttgct attacgccgg
    14641 ataaaatact atccggcttc acaatgggat agttaaatca cgcggcaacc acttcatacg
    14701 agtgagtaat attcaccgct ttttccagca tcaacgccac tgaacaatat ttctcggcag
    14761 agagatcaac cgcacgtgca accgctgcgt ctttcaggtc gcgaccggta acgataaaat
    14821 gcagattaat gtgcgtaaac aggcgtggtg cctcttcgcg gcgttcagag gtcaatttta
    14881 cttcacaatc gaccacatcc tgacgccctt tttgcaggat agaaaccaca tcgatggcac
    14941 tgcaaccccc cgccgccatc aacaccattt ccatcggact tggtgcttta tcgcctgagt
    15001 tgccgtccat taaaatctgg tgaccagagg cggattcgcc cagaaaagtt aacccttcga
    15061 cccacttcac tcgcgcttgc atttttgcta ctccactgcg tcaattttcc tgacagagta
    15121 cgcgtactaa ccaaatcgcg caacggaagg cgacctgggt catgctgaag cgagacacca
    15181 ggagacacaa agcgaaagct atgctaaaac aatcaggatg ctacagtaat acattgatgt
    15241 actgcatgta tgcaaaggac gtcacattac cgtgcagtac agttgatagc cccttcccag
    15301 gtagcgggaa gcatatttcg gcaatccaga gacagcggcg ttatctggct ctggagaaag
    15361 cttataacag aggataaccg cgcatggtgc ttggcaaacc gcaaacagac ccgactctcg
    15421 aatggttctt gtctcattgc cacattcata agtacccatc caagagcacg cttattcacc
    15481 agggtgaaaa agcggaaacg ctgtactaca tcgttaaagg ctctgtggca gtgctgatca
    15541 aagacgaaga gggtaaagaa atgatcctct cctatctgaa tcagggtgat tttattggcg
    15601 aactgggcct gtttgaagag ggccaggaac gtagcgcatg ggtacgtgcg aaaaccgcct
    15661 gtgaagtggc tgaaatttcg tacaaaaaat ttcgccaatt gattcaggta aacccggaca
    15721 ttctgatgcg tctgtctgca cagatggcgc gtcgcctgca agtcacttca gagaaagtgg
    15781 gcaacctggc gttcctcgac gtgacgggcc gcattgcaca gactctgctg aacctggcaa
    15841 aacaaccgga tgctatgact cacccggacg gtatgcaaat caaaattacc cgtcaggaaa
    15901 tcggtcagat tgtcggctgt tctcgtgaaa ccgtgggacg cattctgaag atgctggaag
    15961 atcagaatct gatctccgca cacggtaaaa ccatcgtcgt ttacggcact cgttaatccc
    16021 gtcggagtgg cgcgttacct ggtagcgcgc cattttgttt cccccgatgt ggcgcagact
    16081 gatttatcac cccgatatta actatgcact gcgacaaacg ctggtgctat gtttgcccgt
    16141 ggccgttggg ttaatgcttg gcgaattacg attcggcctg ctcttctccc tcgttcctgc
    16201 ctgttgcaat attgcgggcc ttgatacgcc tcataaacgt tttttcaaac gcttaatcat
    16261 tggtgcgtcg ctgtttgcca cctgtagctt gctgacacag gtgctactgg caaaagatgt
    16321 tcccctgccc tttttgctga ccggattaac gctggtactt ggcgtcactg ctgaactggg
    16381 gccattgcac gcaaaattgc ttcccgcatc gctgctcgcc gccattttta ccctcagtct
    16441 ggcaggatac atgccggtct gggaaccgtt gctcatctat gcgttgggca ctctctggta
    16501 cggattgttt aactggtttt ggttctggat ctggcgcgaa caaccgctgc gcgagtcact
    16561 aagtctgctg taccgcgaac tggcagatta ttgtgaagcc aaatacagcc tgcttaccca
    16621 gcacaccgac cctgaaaaag cgctgccgcc gctgcttgtg cgccagcaaa aagcggtcga
    16681 tctaattacc cagtgctatc agcaaatgca tatgctttcc gcgcaaaata ataccgatta
    16741 caagcggatg ctgcgtattt tccaggaggc gctggatttg caggaacata tttcggtcag
    16801 tttgcatcag ccggaagagg tgcaaaaact ggtcgagcgt agccatgcgg aagaagttat
    16861 ccgctggaat gcgcaaaccg tcgccgctcg cctgcgcgtg ctggccgatg acattcttta
    16921 tcatcgcctg ccgacgcgtt ttacgatgga aaagcaaatt ggcgcactgg aaaaaatcgc
    16981 ccgccagcat ccggataatc cggttgggca attctgctac tggcatttca gccgcatcgc
    17041 ccgcgtgctg cgcacccaaa aaccgctcta tgcgcgtgac ttactggccg ataaacagcg
    17101 gcgaatgccg ttacttccgg cgctgaaaag ttatctgtca ctaaagtctc cggcgctacg
    17161 caatgccgga cgactcagtg tgatgttaag cgttgccagc ctgatgggca ccgcactgca
    17221 tctgccgaag tcgtactgga tcctgatgac ggtattgctg gtgacgcaaa atggctatgg
    17281 cgcaacccgt ctgaggattg tgaatcgctc cgtgggaacc gtggtcgggt taatcattgc
    17341 gggcgtggcg ctgcacttta aaattcccga aggttacacc ctgacgttga tgctgattac
    17401 caccctcgcc agctacctga tattgcgcaa aaactacggc tgggcgacgg tcggttttac
    17461 tattaccgca gtgtataccc tgcaactgtt gtggctgaac ggtgagcagt acatccttcc
    17521 gcgtcttatc gataccatta ttggttgttt aattgctttc ggcggtactg tctggctgtg
    17581 gccgcagtgg cagagcgggt tattgcgtaa gaacgcccat gacgctttag aagcctatca
    17641 ggacgcgatt cgcttgattc tgagcgagga cccgcaacct acgccactgg cctggcagcg
    17701 aatgcgggtg aatcaggcgc ataacactct gtataactca ttgaatcagg cgatgcagga
    17761 accggcgttt aacagccatt atctggcaga tatgaaactg tgggtaacgc acagccagtt
    17821 tattgttgag catattaatg ccatgaccac gctggcgcgg gaacaccggg cattgccacc
    17881 ggaactggca caagagtatt tacagtcttg tgaaatcgcc attcagcgtt gtcagcagcg
    17941 actggagtat gacgaaccgg ggagttctgg cgatgccaat atcatggatg cgccggagat
    18001 gcagccgcac gaaggcgcgg caggtacgct ggagcagcat ttacaacggg ttattggtca
    18061 tctgaacacc atgcacacca tttcgtcgat ggcatggcgg cagcgaccgc atcacgggat
    18121 ttggctgagt cgcaagttgc gggattcgaa ggcgtaatac aggctaaacc atcggatgag
    18181 gcgttcacgc cgcatccgac atcttttgcc tgacgcgtct tatttacgcc ccaatcacct
    18241 tcgccaccgc gtgggcgaaa cgatgcatcc cttcatcgat atccgcatct tccaccacca
    18301 gcgacggcgc aaaacgcatc acatcaggcc cggcattcag caccattacg ccttcctctg
    18361 caccggcata caggaaatca cgcgcccgac ctttgtactg tggtttcagc tccgcgccaa
    18421 ttaacagccc cataccgcga atatcgctaa acacatcgta ctgctgatcg atcttctgca
    18481 aatggtcaac aaaacgctgg cgtttcgcct gaatgccttc cagcacttca ggggtattga
    18541 tgatatcaaa cgccgcgccc gctactgcac aggccagagg attaccgccg taggtggaac
    18601 cgtgagaacc aggatgaaat gcagaagcaa tttccgccgt ggtcagcatg gcgctaatcg
    18661 ggaagccgcc gcctaacgct ttcgcagagg tcagaatatc cggcgtcaca ccgtagtgca
    18721 tgtaagcaaa caaatcgcca gtccgcccca tcccgcactg cacttcatca aacaccaata
    18781 atgcctgatg ttgatcgcac agctcgcgca agccctgcaa aaactctggc gtcgctgccg
    18841 tcacaccgcc ctcgccctgg atcggctcaa ccaccaccgc acaggtatgg tcatccatca
    18901 ccgctttcac tgcgtggaga tcgttaaaag gcacgtggat gatgtctgcc ggttttggcc
    18961 caaagccgtc ggaatatttt ggctgcccac ccaccgaaac ggtaaacagc gagcgaccat
    19021 gaaaagcgtt atggaaggca ataattttgg ttttgaacgg gctatgacgc acacaggcgt
    19081 aatggcgtgc cagtttaaag gcggtttcgt tagcttccgt gccggagttc ataaacacca
    19141 cgcgttcggc aaaagttgcc tcaatcagtt tacggccaag acgcagcgcc ggctcattgg
    19201 tgaaaacgtt actaatatgc cacagagttt cgccctgggt ttttaacgcg ttcaccagcg
    19261 caggatggca atggcccaac gccgtaactg caatgccacc cgcgaaatcg acatactcct
    19321 tgccctgttg atcccagatt cggctgccct gaccttttac cggaataaac tctgccggag
    19381 cataaatcgg cagaatcact tcatcgaaag tcgcgcgtgt aattgctgtt tgttcaattg
    19441 ccatc
//
LOCUS       DAZFLD010000085        19289 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.085, whole genome shotgun sequence.
ACCESSION   DAZFLD010000085 DAZFLD010000000
VERSION     DAZFLD010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19289
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.085"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(72..818)
                     /gene="cutC"
                     /locus_tag="ACHKBL_002527"
     CDS             complement(72..818)
                     /gene="cutC"
                     /locus_tag="ACHKBL_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HFO5819380.1"
                     /translation="MALLEICCYSMECALTAQQNGADRVELCAAPKEGGLTPSLGVLK
                     SVRQRVTIPVHPIIRPRGGDFCYSDGEFAAILEDVRTVRELGFPGLVTGVLDVDGNVD
                     MPRMEKIMAAAGPLAVTFHRAFDMCANPLNTLNNLAELGVARVLTSGQKSDALQGLSK
                     IMELIAHGDAPIIMAGAGVRAENLHHFLDAGVLEVHSSAGAWQASPMRYRNQGLSMSS
                     DAHADEYSRYVVDGAAVAEMKGIIERHQAK"
     gene            complement(832..1398)
                     /gene="yecM"
                     /locus_tag="ACHKBL_002528"
     CDS             complement(832..1398)
                     /gene="yecM"
                     /locus_tag="ACHKBL_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFO5819381.1"
                     /translation="MANWHSIDELQDIASDLPRFTHALDELSRRLGLDITPLTADHIS
                     LRCHQNATAERWRRGFEQCGELLSENMINGRPICLFKLHEPVQVAHWQFSIVELPWPG
                     EKRYPHEGWEHIEIVLPGDPETLNARALALLSDEGLSLPGISVKTSSPKGEHERLPNP
                     TLAVTDGKTTIKFHPWSIEEIVASEQSA"
     gene            1614..3347
                     /gene="argS"
                     /locus_tag="ACHKBL_002529"
     CDS             1614..3347
                     /gene="argS"
                     /locus_tag="ACHKBL_002529"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HFO5819382.1"
                     /translation="MNIQALLSEKVRQAMIAAGAPADCEPQVRQSAKVQFGDYQANGM
                     MAVAKKLGMAPRQLAEQVLTHLDLNGIASKVEIAGPGFINIFLDPAFLAEHVQQALAS
                     DRLGVATPEKQTIVVDYSAPNVAKEMHVGHLRSTIIGDAAVRTLEFLGHKVIRANHVG
                     DWGTQFGMLIAWLEKQQQENAGEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKLQ
                     SGDEYFREMWRKLVDITMTQNQITYDRLNVTLTRDDVMGESLYNPMLPGIVADLKAKG
                     LAVESEGATVVFLDEFKNKEGEPMGVIIQKKDGGYLYTTTDIACAKYRYETLHADRVL
                     YYIDSRQHQHLMQAWAIVRKAGYVPESVPLEHHMFGMMLGKDGKPFKTRAGGTVKLAD
                     LLDEALERARRLVAEKNPDMPADELEKLANAVGIGAVKYADLSKNRTTDYIFDWDNML
                     AFEGNTAPYMQYAYTRVLSVFRKAEIDEEQLAAAPVIIREDREAQLAARLLQFEETLT
                     VVAREGTPHVMCAYLYDLAGLFSGFYEHCPILSAENEEVRNSRLKLAQLTAKTLKLGL
                     DTLGIETVERM"
     gene            complement(3400..3792)
                     /gene="flhe"
                     /locus_tag="ACHKBL_002530"
     CDS             complement(3400..3792)
                     /gene="flhe"
                     /locus_tag="ACHKBL_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlhE"
                     /protein_id="HFO5819383.1"
                     /translation="MRTLLAILLFPLLVQAAGEGMWQASSVGVTLNHRGESMSSAPLS
                     TRQPAAGLMTLVAWRYQLIGPTPAGLRVRLCSQSRCVELEGQSGTTVAFSGIPAIEPL
                     RFIWEVSGGGRLIPPLKVQRNEVIVNYR"
     gene            complement(3792..5870)
                     /gene="flhA"
                     /locus_tag="ACHKBL_002531"
     CDS             complement(3792..5870)
                     /gene="flhA"
                     /locus_tag="ACHKBL_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HFO5819384.1"
                     /translation="MSNLAAMLRLPANLKSTHWQILAGPILILLILSMMVLPLPAFIL
                     DLLFTFNIALSIMVLLVAMFTQRTLEFAAFPTILLFTTLLRLALNVASTRIILMEGHT
                     GAAAAGKVVEAFGHFLVGGNFAIGIVVFVILVIINFMVITKGAGRIAEVGARFVLDGM
                     PGKQMAIDADLNAGLIGEDEAKKRRSEVTQEADFYGSMDGASKFVRGDAIAGILIMVI
                     NVVGGLLVGVLQHGMSMGHAAESYTLLTIGDGLVAQIPALVISTAAGVIVTRVSTDQD
                     VGEQMVNQLFSNPSVMLLSAAVLGLLGLVPGMPNLVFLLFTAGLLGLAWWIRGREQKA
                     PAEPKPVKMAENNTVVEATWNDVQLEDSLGMEVGYRLIPMVDFQQDGELLGRIRSIRK
                     KFAQEMGFLPPVVHIRDNMDLQPARYRILMKGVEIGSGDAYPGRWLAINPGTAAGTLP
                     GEATVDPAFGLNAIWIESALKEQAQIQGYTVVEASTVVATHLNHLISQHAAELFGRQE
                     AQQLLDRVAQEMPKLTEDLVPGVVTLTTLHKVLQNLLDEKVPIRDMRTILETLAEHAP
                     IQSDPHELTAVVRVALGRAITQQWFPGKDEVHVIGLDTPLERLLLQALQGGGGLEPGL
                     ADRLLAQTQEALSRQEMLGAPPVLLVNHALRPLLSRFLRRSLPQLVVLSNLELSDNRH
                     IRMTATIGGK"
     gene            complement(5863..7011)
                     /gene="flhB"
                     /locus_tag="ACHKBL_002532"
     CDS             complement(5863..7011)
                     /gene="flhB"
                     /locus_tag="ACHKBL_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhB"
                     /protein_id="HFO5819385.1"
                     /translation="MSDESDDKTEAPTPHRLEKAREEGQIPRSRELTSLLILLVGVCV
                     IWFGGVSLARRLSGMLSAGLHFDHSIINDPNLILGQIILLIREAMLALLPLISGVVLV
                     ALISPVMLGGLVFSGKSLQPKFSKLNPLPGIKRMFSAQTGAELLKAILKSILVGSVTG
                     FYLWHHWPQMMRLMAESPITAMGNAMDLVGLCALLVVLGVIPMVGFDVFFQIFSHLKK
                     LRMSRQDIRDEFKQSEGDPHVKGRIRQMQRAAARRRMMADVPKADVIVNNPTHYSVAL
                     QYDENKMSAPKVVAKGAGLVALRIREIGAENNVPTLEAPPLARALYRHAEIGQQIPGQ
                     LYAAVAEVLAWVWQLKRWRLAGGQRPVQPTHLPVPEALDFINEKPTHE"
     gene            complement(7200..7844)
                     /gene="cheZ"
                     /locus_tag="ACHKBL_002533"
     CDS             complement(7200..7844)
                     /gene="cheZ"
                     /locus_tag="ACHKBL_002533"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0050920 - regulation of chemotaxis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HFO5819386.1"
                     /translation="MMQPSIKPADEHSAGDIIARIGSLTRMLRDSLRELGLDQAIAEA
                     AEAIPDARDRLYYVVQMTAQAAERALNSVEASQPHQDQMEKSAKALTQRWDDWFADPI
                     DLADARELVTDTRQFLADVPAHTSFTNAQLLEIMMAQDFQDLTGQVIKRMMDVIQEIE
                     RQLLMVLLENIPEQESRPKRENQSLLNGPQVDTSKAGVVASQDQVDDLLDSLGF"
     gene            complement(7855..8244)
                     /gene="cheY"
                     /locus_tag="ACHKBL_002534"
     CDS             complement(7855..8244)
                     /gene="cheY"
                     /locus_tag="ACHKBL_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002210873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator CheY"
                     /protein_id="HFO5819387.1"
                     /translation="MADKELKFLVVDDFSTMRRIVRNLLKELGFNNVEEAEDGVDALN
                     KLQAGGYGFVISDWNMPNMDGLELLKTIRADGAMSALPVLMVTAEAKKENIIAAAQAG
                     ASGYVVKPFTAATLEEKLNKIFEKLGM"
     gene            complement(8259..9308)
                     /gene="cheB"
                     /locus_tag="ACHKBL_002535"
     CDS             complement(8259..9308)
                     /gene="cheB"
                     /locus_tag="ACHKBL_002535"
                     /EC_number="3.1.1.61"
                     /EC_number="3.5.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate methylesterase/protein
                     glutamine deamidase"
                     /protein_id="HFO5819388.1"
                     /translation="MSKIRVLSVDDSALMRQIMTEIINSHSDMEMVATAPDPLVARDL
                     IKKFNPDVLTLDVEMPRMDGLDFLEKLMRLRPMPVVMVSSLTGKGSEVTLRALELGAI
                     DFVTKPQLGIREGMLAYSEMIAEKVRTAAKASLAAHKPLSAPTTLKAGPLLSSEKLIA
                     IGASTGGTEAIRHVLQPLPLSSPALLITQHMPPGFTRSFADRLNKLCQIGVKEAEDGE
                     RVLPGHAYIAPGDRHMELARSGANYQIKIHDGPAVNRHRPSVDVLFHSVAKQAGRNAV
                     GVILTGMGNDGAVGMLAMRQAGAWTLAQNEASCVVFGMPREAINMGGVCEVVDLSQVS
                     QQMLAKISAGQAIRI"
     gene            complement(9311..10171)
                     /gene="cheR"
                     /locus_tag="ACHKBL_002536"
     CDS             complement(9311..10171)
                     /gene="cheR"
                     /locus_tag="ACHKBL_002536"
                     /EC_number="2.1.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HFO5819389.1"
                     /translation="MTSSLPCGQTSLLLQMTERLALSDAHFRRISQLIYQRAGIVLAD
                     HKRDMVYNRLVRRLRSLGLTDFGHYLNLLESNLHSGEWQAFINSLTTNLTAFFREAHH
                     FPLLADHARRRSGEYRVWSAAASTGEEPYSIAMTLADTLGTVPGRWKVFASDIDTEVL
                     EKARSGIYRHEELKNLTPQQLQRYFMRGTGPHEGLVRVRQELANYVDFAPLNLLAKQY
                     TVPGPFDAIFCRNVMIYFDQTTQQEILRRFVPLLKPDGLLFAGHSENFSHLERRFTLR
                     GQTVYALSKD"
     gene            complement(10190..11791)
                     /gene="tap"
                     /locus_tag="ACHKBL_002537"
     CDS             complement(10190..11791)
                     /gene="tap"
                     /locus_tag="ACHKBL_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein IV"
                     /protein_id="HFO5819390.1"
                     /translation="MFNRIRISTTLFLILILCGILQIGSNGMSFWAFRDDLQRLNQVE
                     QSNQQRAALAQTRAVMLQASTALNKAGTLTALSYPADDIKTLMTTARASLTQSTTLFK
                     SFMAMTAGNEHVRALQKETEKSFARWHNDLEHQATWLESNQLSDFLTAPVQESQNAFD
                     VNFEAWQLEINHVLESASAQSQRNYHISALVFISMIIVAAIYISSALWWTRKMIVQPL
                     AIIGSHFDSIAAGNLARPIAVYGRNEITAIFASLKTMQQALRGTVSDVRKGSQEMHIG
                     IAEIVAGNNDLSSRTEQQAASLAQTAASMEQLTATVGQNADNARQASELAKNAATTAQ
                     AGGVQVSTMTHTMQEIATSSQKIGDIISVIDGIAFQTNILALNAAVEAARAGEQGRGF
                     AVVAGEVRNLASRSAQAAKEIKGLIEESVNRVQQGSKLVNNAAATMTDIVSSVTRVND
                     IMGEIASASEEQRRGIEQVAQAVSQMDQVTQQNASLVEEAAVATEQLANQADHLSSRV
                     AVFTLEEHEVARHESAQLQIAPVVS"
     gene            complement(11837..13498)
                     /gene="tar"
                     /locus_tag="ACHKBL_002538"
     CDS             complement(11837..13498)
                     /gene="tar"
                     /locus_tag="ACHKBL_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004888 - transmembrane signaling receptor
                     activity [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein II"
                     /protein_id="HFO5819391.1"
                     /translation="MINRIRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSN
                     QLREQQGELTSTWDLMLQTRINLSRSAVRMMMDSSNQQSNAKVELLDSARKTLAQAAT
                     HYKKFKSMAPLPEMVATSRNIDEKYKNYHTALTELIDYLDYGNTGAYFAQPTQGMQNA
                     MGEAFAQYALSSEKLYRDIVTDNADDYRFAQWQLAVIALVVVLILLVAWYGIRRMLLT
                     PLAKIIAHIREIAGGNLANTLTIDGRSEMGDLAQSVSHMQRSLTDTVTHVREGSDAIY
                     AGTREIAAGNTDLSSRTEQQASALEETAASMEQLTATVKQNADNARQASQLAQSASDT
                     AQHGGKVVDGVVKTMHEIADSSKKIADIISVIDGIAFQTNILALNAAVEAARAGEQGR
                     GFAVVAGEVRNLASRSAQAAKEIKALIEDSVSRVDTGSVLVESAGETMNNIVNAVTRV
                     TDIMGEIASASDEQSRGIDQVALAVSEMDRVTQQNASLVQESAAAAAALEEQASRLTQ
                     AVSAFRLAASPLTNKPQTPSRPASEQPPAQPRLRIAEQDPNWETF"
     gene            complement(13643..14146)
                     /gene="cheW"
                     /locus_tag="ACHKBL_002539"
     CDS             complement(13643..14146)
                     /gene="cheW"
                     /locus_tag="ACHKBL_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HFO5819392.1"
                     /translation="MTGMTNVTKLASEPSGQEFLVFTLGDEEYGIDILKVQEIRGYDQ
                     VTRIANTPAFIKGVTNLRGVIVPIVDLRIKFSQVDVDYNDNTVVIVLNLGQRVVGIVV
                     DGVSDVLSLTAEQIRPAPEFAVTLSTEYLTGLGALGDRMLILVNIEKLLNSEEMALLD
                     SAASEVA"
     gene            complement(14167..16131)
                     /gene="cheA"
                     /locus_tag="ACHKBL_002540"
     CDS             complement(14167..16131)
                     /gene="cheA"
                     /locus_tag="ACHKBL_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HFO5819393.1"
                     /translation="MSMDISDFYQTFFDEADELLADMEQHLLVLQPEAPDAEQLNAIF
                     RAAHSIKGGAGTFGFSVLQETTHLMENLLDEARRGEMQLNTDIINLFLETKDIMQEQL
                     DAYKQSQEPDAASFDYICQALRQLALEAKGETPSAVTRLSVVAKSEPQDEQSRDQSPR
                     RIILSRLKAGEVDLLEEELGHLTTLTDVVKGADSLSAILPGDIAEDDITAVLCFVIEA
                     DQITFETVEVSPKISPPPVLKLAAEQAPTGRVEREKTTRSSESTSIRVAVEKVDQLIN
                     LVGELVITQSMLAQRSSELDPVNHGDLITSMGQLQRNARDLQESVMSIRMMPMEYVFS
                     RYPRLVRDLAGKLGKQVELTLVGSSTELDKSLIERIIDPLTHLVRNSLDHGIELPEKR
                     LAAGKNSVGNLILSAEHQGGNICIEVTDDGAGLNRERILAKAASQGLTVSENMSDDEV
                     AMLIFAPGFSTAEQVTDVSGRGVGMDVVKRNIQEMGGHVEIQSKQGTGTTIRILLPLT
                     LAILDGMSVRVADEVFILPLNAVMESLQPREADLHPLAGGERVLEVRGEYLPIVELWK
                     VFNVAGAKTEATQGIVVILQSGGRRYALLVDQLIGQHQVVVKNLESNYRKVPGISAAT
                     ILGDGSVALIVDVSALQAINREQRMANTAA"
     gene            complement(16136..17062)
                     /gene="motB"
                     /locus_tag="ACHKBL_002541"
     CDS             complement(16136..17062)
                     /gene="motB"
                     /locus_tag="ACHKBL_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HFO5819394.1"
                     /translation="MKNQAHPIIVVKRRKAKSHGAAHGSWKIAYADFMTAMMAFFLVM
                     WLISISSPKELIQIAEYFRTPLATAVTGGDRISNSESPIPGGGDDYTQSQGEVNKQPN
                     IEELKKRMEQSRLRKLRGDLDQLIESDPKLRALRPHLKIDLVQEGLRIQIIDSQNRPM
                     FRTGSADVEPYMRDILRAIAPVLNGIPNRISLSGHTDDFPYASGEKGYSNWELSADRA
                     NASRRELMVGGLNGGKVLRVVGMAATMRLSDRGPDDAVNRRISLLVLNKQAEQAILHE
                     NAESQNEPVSALEKPEVAPQVSVPTMPSAEPR"
     gene            complement(17059..17946)
                     /gene="motA"
                     /locus_tag="ACHKBL_002542"
     CDS             complement(17059..17946)
                     /gene="motA"
                     /locus_tag="ACHKBL_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HFO5819395.1"
                     /translation="MLILLGYLVVLGTVFGGYLMTGGSLGALYQPAELVIIAGAGIGS
                     FIVGNNGKAIKGTLKALPLLFRRSKYTKAMYMDLLALLYRLMAKSRQMGMFSLERDIE
                     NPRESEIFASYPRILADSVMLEFIVDYLRLIISGHMNTFEIEALMDEEIETHESEAEV
                     PANSLALVGDSLPAFGIVAAVMGVVHALGSADRPAAELGALIAHAMVGTFLGILLAYG
                     FISPLASVLRQKSAETSKMMQCVKVTLLSNLNGYAPPIAVEFGRKTLYSSERPSFIEL
                     EEHVRAVKNPQQQTTTEEA"
     gene            complement(18073..18651)
                     /gene="flhC"
                     /locus_tag="ACHKBL_002543"
     CDS             complement(18073..18651)
                     /gene="flhC"
                     /locus_tag="ACHKBL_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhC"
                     /protein_id="HFO5819396.1"
                     /translation="MSEKSIVQEARDIQLAMELITLGARLQMLESETQLSRGRLIKLY
                     KELRGSPPPKGMLPFSTDWFMTWEQNVHASMFCNAWQFLLKTGLCNGVDAVIKAYRLY
                     LEQCPQAEEGPLLALTRAWTFVRFVESGLLQLSSCNCCGGNFITHAHQPVGSFACSLC
                     QPPSRAVKRRKLSQNPADIIPQLLDEQRVQAV"
     gene            complement(18654..19004)
                     /gene="flhD"
                     /locus_tag="ACHKBL_002544"
     CDS             complement(18654..19004)
                     /gene="flhD"
                     /locus_tag="ACHKBL_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhD"
                     /protein_id="HFO5819397.1"
                     /translation="MHTSELLKHIYDINLSYLLLAQRLIVQDKASAMFRLGINEEMAT
                     TLAALTLPQMVKLAETNQLVCHFRFDSHQTITQLTQDSRVDDLQQIHTGIMLSTRLLN
                     DVNQPEEALRKKRA"
BASE COUNT         4546 a         5414 c         4758 g         4571 t
ORIGIN      
        1 gcccctgttg aaattgcagg ggcctggtac gagcaagcat catattgggc gacatgatgc
       61 aacggtaaaa atcatttggc ctgatggcgt tcaatgattc ctttcatttc agcaaccgcc
      121 gccccgtcta cgacataacg cgaatactcg tccgcgtgtg catctgatga catggacaat
      181 ccttgattac gataacgcat tggtgaggct tgccacgctc ccgcggagct atggacttcc
      241 agcactccgg catcgaggaa gtggtgcagg ttttctgcac ggactcctgc tccggccata
      301 atgattggag catcaccatg ggcaataagt tccataattt ttgataaacc ttgcagtgcg
      361 tctgattttt gccctgatgt cagtacccga gcaacgccca attccgcaag attattgagt
      421 gtatttaaag ggttagcgca catatcgaag gcgcgatgaa aagtcacggc cagcggaccg
      481 gcagcagcca ttattttttc cattcgtggc atatcgacat tcccgtcaac atcgagaaca
      541 cccgtcacca gtccaggaaa acctaattcg cggactgtgc gcacatcctc aagaatggcg
      601 gcaaactcac cgtcgctgta gcaaaaatca ccaccgcgtg ggcgaataat aggatgcaca
      661 gggatcgtca cccgctggcg cacggatttc agcaccccca acgacggcgt taagcccccc
      721 tcttttgggg ctgcgcataa ttcaactctg tctgcgccgt tttgctgcgc cgttagcgcg
      781 cattccatgc tgtaacagca aatttccagt aatgccattt ttgctcctta attacgccga
      841 ctgctcgctg gcaacgatct cttcaatgga ccacggatga aatttaatgg tcgttttgcc
      901 atcggtgacc gccagcgtcg gattcggtaa tcgctcatgc tcaccttttg gcgaactggt
      961 tttaactgag atccccggca ggctgagtcc ttcatcagaa agcaatgcca gcgcacgggc
     1021 attcagcgtt tctggatcgc ccggcagaac gatttcaata tgttcccaac cttcgtgcgg
     1081 gtaacgtttt tccccgggcc acggtagctc cacaatggaa aactgccagt gcgcaacctg
     1141 taccggttca tgcaatttaa acagacaaat cggcctgcca ttgatcatat tttctgacaa
     1201 aagctcgccg cactgttcaa acccgcgacg ccagcgctca gcggtggcgt tttgatggca
     1261 acgcaaagaa atgtgatcgg cagtcagcgg agtgatatcc aaccccaaac ggcgggaaag
     1321 ttcatctaat gcgtgggtaa atcgcggtaa atccgatgca atatcctgca gctcgtcgat
     1381 agaatgccag ttcgccataa tcactcttcg tcttccacta aaagcgttaa tttaccctgt
     1441 tgccctgcgc caaccaaccg ctgatttcac gccgcttctg atgcaatagt gaaaacgaca
     1501 atacgccacg cgcacgttgc tgacgaaaac agccatttgc agtatactcc cgccctaatt
     1561 tctttaactg gtgcgggtaa tttttgctcg cttcatcaat gtaaggtatt ccggtgaata
     1621 ttcaggctct tctctcagaa aaagtccgtc aggccatgat tgcggcaggc gcgcctgcgg
     1681 attgcgaacc gcaggttcgt cagtcagcaa aagttcagtt cggcgactat caagctaacg
     1741 gcatgatggc agttgctaaa aaactgggta tggcaccgcg acaattagca gagcaggtgc
     1801 tgactcatct ggatcttaac ggtatcgcca gcaaagttga gatcgcgggt cctggcttta
     1861 tcaacatttt ccttgatccg gcattcctgg ctgaacatgt tcagcaggcg ctggcgtccg
     1921 atcgtctcgg tgttgctacg ccagaaaaac agaccattgt ggttgactac tctgcgccaa
     1981 acgtggcgaa agagatgcac gttggtcacc tgcgctctac cattattggt gacgcggcag
     2041 tgcgtactct ggagttcctc ggtcacaaag tgattcgcgc aaaccacgtc ggcgactggg
     2101 gcactcagtt cggtatgttg attgcgtggc tggaaaagca gcagcaggaa aacgccggtg
     2161 aaatggagct ggctgacctt gaaggtttct accgcgatgc gaaaaagcat tacgacgaag
     2221 atgaagagtt tgccgagcgt gcacgtaact acgtggtaaa actgcaaagc ggtgacgaat
     2281 atttccgcga gatgtggcgc aaactggtcg acatcaccat gacgcagaac cagatcacct
     2341 acgatcgtct caacgtgacg ctgacccgtg atgacgtgat gggtgaaagc ctctacaacc
     2401 caatgctgcc gggtatcgtg gcggacctga aagccaaagg tctggcagta gaaagtgaag
     2461 gcgcgaccgt cgtattcctt gatgagttca aaaacaagga aggcgaaccg atgggcgtga
     2521 tcattcagaa gaaagatggt ggctatctct acaccaccac tgatatcgcc tgtgcgaaat
     2581 atcgttatga aacactgcat gccgatcgcg tgctgtatta catcgactcc cgtcagcatc
     2641 aacacctgat gcaggcgtgg gctatcgtcc gtaaagcagg ctatgtaccg gaatccgtac
     2701 cgctggaaca ccacatgttc ggcatgatgc tgggcaaaga cggcaaaccg ttcaaaaccc
     2761 gcgcgggtgg tacagtgaaa ctggccgatc tgctggatga agccctggaa cgtgcacgcc
     2821 gtctggtggc agaaaagaac ccggatatgc cagccgacga gctggaaaaa ctggctaacg
     2881 cggttggtat tggtgcggtg aaatatgcgg atctctccaa aaaccgcacc acggactaca
     2941 tcttcgactg ggacaacatg ctggcgtttg agggtaatac cgcgccatac atgcagtatg
     3001 catacacgcg tgtattgtcc gtgttccgta aagcagaaat tgacgaagag caactggctg
     3061 cagctccggt tatcatccgt gaagatcgtg aagcgcaact ggcagctcgc ctgctgcagt
     3121 ttgaagaaac cctcaccgtg gttgcccgtg aaggcacgcc gcatgtaatg tgtgcttacc
     3181 tgtacgatct ggccggtctg ttctctggtt tctacgagca ctgcccgatc ctcagcgcag
     3241 aaaacgaaga agtgcgtaac agccgtctga aactggcaca actgacggcg aagacgctga
     3301 aactgggtct ggatacgctg ggtattgaga cagtagagcg tatgtaatcg cgttatacgg
     3361 caaaagccga tgtcatggca gacatcggct tttttatcat taacgataat tcacaatcac
     3421 ttcattccgt tgtaccttta gcggcggaat taaccgccca ccgccagaca cttcccagat
     3481 aaatcgcaac ggttctatcg ccggtatacc ggaaaaggcc acggtggttc cactctgccc
     3541 ttctaattcg acacaacgag attgtgaaca caagcgaacc cgcagtcctg caggtgtcgg
     3601 gccgataagc tgataacgcc acgccaccag cgtcatcaat cctgcagcag gttgtcgagt
     3661 agaaagaggc gctgacgaca tcgactcacc acggtgattc agcgtaacgc ccacactact
     3721 cgcctgccac atcccctccc cggcggcttg caccagcagc ggaaacaata atattgctaa
     3781 taaagttctc atcatttacc gccaattgtc gccgtcatgc ggatatgtcg gttatcagat
     3841 agttccagat tcgacagcac cactaactgc ggcaggctgc ggcgcaggaa gcgagacaat
     3901 aatggtcgca gcgcgtggtt caccaacaat accggcggcg cacccaacat ctcctgacgg
     3961 gatagcgctt cctgagtttg cgccagtaaa cgatccgcca gccctggctc cagtcccccc
     4021 ccgccctgca acgcctgtag caacaaacgt tccagcggtg tatcgaggcc aataacatgg
     4081 acttcatctt tgccaggaaa ccactgctgg gtaatcgccc ggcccaacgc tacgcgcacg
     4141 acggcggtta attcatgcgg atcgctttgg atgggcgcat gttccgccag cgtttcgaga
     4201 atggtgcgca tatcgcgaat cggcactttt tcatcgagca gattttgcag cactttatgc
     4261 agtgtggtaa gcgtgacgac accaggaacg agatcttccg tcagctttgg catctcctgg
     4321 gcgacgcgat ccagcagctg ttgtgcctcc tgacgaccaa acagctctgc ggcatgctgg
     4381 ctaatgaggt ggttaagatg ggttgccacc accgtgctgg cctcaaccac ggtgtacccc
     4441 tgaatctgcg cctgttcttt aagcgcactt tcaatccaga tagcattcag gccaaatgcc
     4501 ggatcgacgg tcgcctcacc aggtaacgtc ccggcagcgg taccagggtt aatcgccaac
     4561 caacgccccg gataagcatc accactgcct atctccacgc ctttcatcaa aatgcgatag
     4621 cgggcaggtt gcagatccat attgtcgcga atgtgcacca ccggcggcag aaaccccatc
     4681 tcctgggcaa atttcttgcg aatactgcgt atacggccca gcaactcacc atcctgctgg
     4741 aaatcgacca tcgggatcag gcgataaccc acttccattc ccagagaatc ttccagttgc
     4801 acatcgttcc acgtcgcttc gacaacggta ttattctctg ccatttttac cggttttggt
     4861 tcggcaggcg ctttttgttc acgtccgcgg atccaccagg ccagtcccag caatccggca
     4921 gtgaacagca aaaataccag gttcggcatt ccaggcacca ggccgagtaa accgagcacg
     4981 gcggcgctta acaacataac gcttgggtta ctgaaaagct gattcaccat ctgctcgcca
     5041 acatcctgat cggtgctgac acgcgtaacg atgacccccg cggcggtaga aatcaccaac
     5101 gccggaattt gtgccaccag accgtcgcca atagtcaata gcgtataact ttccgccgcg
     5161 tgtcccatgc tcataccatg ttgcagcacg ccgaccagca acccaccgac aacgtttatg
     5221 accatgatga ggatcccggc gatggcatcg ccgcgaacaa acttacttgc cccgtccatc
     5281 gagccgtaaa aatcggcttc ctgagtcact tcggagcggc gttttttcgc ttcatcttca
     5341 ccaatcaatc cggcgttaag gtcggcgtca atcgccatct gcttccccgg cataccatcg
     5401 agaacaaagc gcgcgcctac ttccgcaata cgcccggcac ctttggtaat gaccataaag
     5461 ttgatgatca cgagaatgac gaacaccacg ataccgatag cgaaattgcc accaacgagg
     5521 aagtgaccga acgcttcgac cacctttcca gccgccgccg cgccggtatg cccttccatc
     5581 aaaatgatac gggttgaagc cacattaagt gccagacgca acaacgtggt aaacaacaga
     5641 atcgtcggaa acgcagcaaa ctcaagcgtg cgttgggtaa acatcgccac cagcaacacc
     5701 atgatcgaca aggcaatatt gaaggtaaac agcaggtcga gtatgaatgc gggcagtggc
     5761 agtaccatca tcgacaagat caacaggatc aaaatcggtc cggcaaggat ctgccagtgt
     5821 gtcgatttca ggtttgcggg caggcgcagc atcgcggcca gattactcat gggtcggttt
     5881 ctcgttaata aaatccaggg cttccggcac cggaagatga gtaggttgta cagggcgctg
     5941 tccaccagcc agacgccagc gtttcagttg ccagacccag gccagcactt ccgccaccgc
     6001 ggcgtacagt tgacccggga tttgttgacc gatctccgca tgtcgataca gcgcacgcgc
     6061 cagcggcggc gcttcaagcg tcgggacgtt attttcagcg ccaatttcac gaatgcgcag
     6121 cgcgaccagc cctgcacctt tagcgaccac tttcggtgcg ctcattttgt tttcgtcata
     6181 ctgcaaagcc actgagtagt gagttgggtt attgacgata acgtccgctt tcggcacatc
     6241 ggccatcatc cgccgccgcg ccgcagctcg ctgcatctga cggattcgcc ctttgacatg
     6301 agggtcgcct tcgctttgtt tgaactcatc acgaatatcc tgccgtgaca tacgcagctt
     6361 tttcaggtgg ctgaagattt ggaaaaagac gtcaaatccc accatcggaa tgacaccaag
     6421 caccaccagc aatgcgcaca gccctaccag atccatcgca ttacccatgg cggtaatggg
     6481 ggactccgcc attaagcgca tcatttgcgg ccagtggtgc cagagataaa accccgtcac
     6541 actgccgacc aggatggatt tcaaaatcgc tttaagcaac tccgcaccgg tttgtgccga
     6601 aaacatccgt ttaatgcccg gtagcgggtt gagtttggaa aacttcggct gcaaggattt
     6661 gccgctaaat accaggccac ccaacataac cggagaaata agcgccacca gcaccacacc
     6721 gctaatcagc ggcagcagcg ccagcatggc ttctctgatc agcagaataa tttgcccgag
     6781 gatcagattc gggtcattga taatactgtg atcaaaatgc agcccagcgg agagcatgcc
     6841 cgacaatcga cgggccagcg acacaccgcc aaaccagata acacacacgc ccactaacaa
     6901 aatcagcagt gacgttaatt cacgtgaacg cgggatttgc ccctcttccc gcgctttttc
     6961 tagtcggtga ggtgtggggg cttctgtttt gtcgtcgctc tcgtcagaca cgtcgccaat
     7021 cctggaaaga gtattaagcg taaatgatgc cagagcgcaa agcgttcaat ggtttgagta
     7081 aggggcaaaa caggcgggat ttagggcttt tactgccaca catcaagcat agtatgcgtt
     7141 tgtcggatgc ggcgtcatcg cctgatatga cgtggtcacg ccacatcagg caatacaaat
     7201 caaaatccaa gactatccaa caaatcgtcc acctgatcct gactggctac cacaccggct
     7261 ttgctggtat cgacctgagg tccattaagc aaactctggt tttcacgttt tggacgcgac
     7321 tcctgttccg ggatgttttc caacagcacc atcaacaact ggcgttcgat ctcctgaatg
     7381 acatccatca tccgcttaat aacctgcccg gtgagatcct gaaaatcctg cgccatcatg
     7441 atttccagca gttgcgcgtt agtaaagctg gtatgcgcgg ggacatctgc cagaaattgc
     7501 cgcgtatctg ttaccagttc tcgggcgtcg gcaaggtcaa tcggatcggc aaaccagtca
     7561 tcccaacgtt gggttaacgc ttttgctgat ttctccattt gatcctgatg cggttgtgac
     7621 gcctcaacac tgttcagcgc ccgctccgca gcctgggcag tcatctgcac aacatagtac
     7681 aaacgatcgc gcgcatcggg gatggcttcc gccgcttcgg caatggcctg atccagcccc
     7741 agttcccgca aactgtcgcg cagcatacgc gtcaggctgc cgatgcgcgc aatgatatcg
     7801 ccagctgaat gctcgtcagc aggtttgatt gatggttgca tcatagtcgc atcctcacat
     7861 gcccagtttc tcaaagattt tgttgagttt ttcctccagc gtcgcggcgg taaatggctt
     7921 caccacatag ccactggccc ccgcttgcgc cgcagcaata atgttctctt tcttcgcttc
     7981 tgcagtcacc attaacactg gcaatgctga catcgcgcca tccgcacgaa tcgttttcag
     8041 caattccagg ccatccatgt tgggcatgtt ccagtcggag ataacaaatc cataaccgcc
     8101 tgcctgcaac ttattgagag cgtcgacacc atcttccgct tcctcaacat tattgaatcc
     8161 tagctctttc agcaggttac gcactatgcg tcgcatggtg gaaaagtcat ccacaaccaa
     8221 aaatttaagt tctttatccg ccatttcaca ctcctgattt aaatacgtat cgcctgtccg
     8281 gcactaattt ttgccagcat ttgctggctt acctggctaa gatcgaccac ttcgcaaaca
     8341 ccacccatat tgatggcctc gcgcggcatg ccgaacacca cgcaacttgc ttcgttttgt
     8401 gcaagggtcc atgcccccgc ctgacgcatc gccaacattc ccaccgcgcc gtcgttgccc
     8461 ataccggtca ggatcacccc aaccgcatta cgccccgcct gtttggcgac agaatggaac
     8521 aacacatcta ccgaaggccg atgacggtta accgccgggc catcgtgaat tttgatttgg
     8581 taatttgcgc cactacgcgc cagctccata tgccgatcgc ccggcgcaat ataagcatgc
     8641 cccggcaaga cacgttctcc gtcttcggct tctttaaccc cgatctggca aagcttatta
     8701 agtctgtcgg caaaagagcg ggtgaaaccg ggcggcatat gctgggtaat taacagtgcc
     8761 gggctggaaa gcggcaacgg ttgcagtacg tgacgaattg cctcagttcc acccgttgaa
     8821 gcaccaatcg caatcagttt ttcagagctc aacaacggcc ctgccttcag cgttgtcggt
     8881 gccgacaatg gcttatgtgc tgcaaggctc gcctttgctg ctgtacgcac cttttcagca
     8941 atcatttcgc tatacgccag cattccttcg cgaataccca gttgcggttt ggtgacaaaa
     9001 tctatcgccc ccagctccaa tgcgcgcagc gtgacttctg atcctttgcc ggtcagggaa
     9061 gaaaccatca caacgggcat tgggcgcaaa cgcattaatt tttcgagaaa atccagtccg
     9121 tccatccgcg gcatttcaac atccagcgtt agcacatccg gattgaattt cttaatcaaa
     9181 tcacgcgcaa ccagcggatc aggggcggtc gccaccattt ccatgtcgct atggctgttg
     9241 atgatttctg tcatgatctg gcgcatcagt gccgaatcat cgacagataa caccctgatt
     9301 ttgctcatcg ttaatcctta cttagcgcat acaccgtctg accacgcagc gtgaagcggc
     9361 gctcaaggtg gctaaagttt tcagagtgac ccgcaaacag caatccgtcg ggtttaagga
     9421 gcggaacaaa gcggcgcaaa atctcctgct gggtagtttg atcgaagtag atcatgacgt
     9481 tacgacagaa gatcgcatca aacggccccg gcacggtgta ctgtttcgcc agtaaattca
     9541 gcggggcaaa atcaacatag ttcgccagct cctgacgcac gcgtaccagc ccttcatgcg
     9601 gtcccgtccc tcgcatgaaa taccgttgca gttgctgcgg cgtcaggttt ttcaactctt
     9661 catggcgata aataccgctt ctggcttttt ccagcacttc ggtgtcgata tcgctggcaa
     9721 acactttcca gcgtccgggc acggtgccca atgtgtcagc cagcgtcatc gcaatgctgt
     9781 acggctcttc gccggtcgaa gccgccgcgc tccatacgcg atactcgcca gaacggcgac
     9841 gtgcgtgatc cgcgagcaga ggaaaatgat gcgcctcacg gaaaaatgcc gtcagattcg
     9901 tggtcagcga attgataaac gcctgccact caccgctgtg cagattagat tccagcaagt
     9961 tcaggtaatg accgaaatcc gtcagtccca gcgaacgcaa acgacgaacc agtcggttgt
    10021 aaaccatgtc gcgtttatgg tcggccagga cgatcccggc tcgttgatag atcaattgac
    10081 ttatccgccg aaaatgcgcg tcggaaagcg ccaggcgctc ggtcatctgt aacaataaag
    10141 acgtttgccc acagggcaga gatgaagtca tagcgccttc tcaatcactt caggatacca
    10201 ctggcgcaat ttgtaactgc gccgactcat gtcgtgctac ttcatgctct tcaagggtaa
    10261 ataccgccac gcgcgacgaa agatggtcgg cctgattcgc cagttgttct gttgccaccg
    10321 ccgcttcttc taccagggag gcgttctgct gtgtcacctg atccatctgg ctgacagcct
    10381 gtgcaacctg ctcaatcccc cgccgttgtt cttccgaggc cgaagcaatt tctcccataa
    10441 tgtcgttcac gcgggtcacc gaactgacaa tatcagtcat ggtcgcggcg gcgttattca
    10501 ccagtttcga accctgctgg acacgattga ctgactcttc gatcagccct ttgatctctt
    10561 ttgctgcctg ggcgctacgg ctggcaagat tgcgcacttc acctgccact accgcaaaac
    10621 cacgcccctg ctctccggcg cgagccgctt ccactgccgc attcagggcc agaatattgg
    10681 tctggaaagc aatgccgtcg ataacgctga taatgtcgcc aattttttgc gagctggtag
    10741 cgatctcctg catggtgtga gtcatggtac tgacctgaac accgcccgcc tgcgccgttg
    10801 tcgcggcatt ttttgccagt tccgacgcct gtcgtgcgtt atcggcgttt tgccctaccg
    10861 tggcggttaa ttgctccata ctggcggccg tttgtgccag cgatgccgcc tgctgttcgg
    10921 tgcgacttga gagatcgtta ttgcctgcga caatctccgc gataccaatg tgcatctcct
    10981 ggcttccctt acgcacatca cttaccgtcc cacgcaaagc ctgctgcatg gtcttcaggc
    11041 tggcaaaaat ggcggtgatc tcattgcgac catacaccgc aatcggacgt gccagattac
    11101 ccgcagcaat gctgtcaaaa tggctaccga taatggccag tggttgaaca atcatcttgc
    11161 gcgtccacca cagcgcactg ctgatgtaga tcgccgcaac aataatcatg ctgataaaca
    11221 ccagcgccga aatgtgatag ttacgctggc tttgcgcact ggcactttcc agcacatggt
    11281 tgatctccag ctgccaggcc tcaaagttaa cgtcaaacgc attctgtgat tcctgcaccg
    11341 gcgcagtgag gaaatccgaa agttgattac tttcaagcca ggtcgcctga tgttcgagat
    11401 cgttgtgcca gcgggcaaaa cttttctccg tctctttttg caacgccctg acgtgctcgt
    11461 tgcccgcagt catcgccata aaacttttaa acagcgtggt ggattgcgtc agactggcgc
    11521 gcgccgtcgt catcaacgtt ttaatgtcat ccgccggata gctaagcgcc gtcagagtgc
    11581 ccgctttgtt cagcgcggta ctggcctgta acattaccgc ccgagtttgc gctaatgccg
    11641 cacgttgctg attgctctgc tcgacctgat tcagtcgttg caaatcgtcg cgaaatgccc
    11701 aaaaagacat gccgttactg ccaatctgca agatcccgca gagaatcaaa attaaaaaca
    11761 gcgtggtcga aattcgaata cgattaaaca tcaacgctcc ccatcaggcg gcaatgaccg
    11821 cgttagtaaa tactcgtcaa aatgtttccc agtttggatc ttgttcagca attcgcagtc
    11881 gtggctgtgc cggtggttgc tcactggcag gacgggatgg tgtttgcggt ttattggtga
    11941 gtgggctggc tgccagacgg aacgcggaaa ccgcttgcgt taaacgactc gcctgttctt
    12001 ccagcgcagc tgcggcggca gctgattcct gcaccagcga tgcgttctgt tgcgtgacgc
    12061 gatccatttc cgaaaccgcc aatgcgactt gatcgatgcc acggctctgt tcatccgatg
    12121 ccgatgcaat ctcgcccata atgtcagtca cgcgggtgac agcattgacg atattgttca
    12181 ttgtttcccc ggcgctttcg accagcaccg aaccggtatc aacgcgcgag acggagtctt
    12241 caatgagggc tttgatctct tttgccgcct gggcgctgcg actggcaaga ttacgcactt
    12301 cgcccgccac cacggcaaaa ccacggccct gttcacccgc tcgcgcggct tcaacagcgg
    12361 cattcagcgc gagtatatta gtctggaagg caataccatc gataacgctg ataatgtcgg
    12421 caattttctt cgaactatcg gcgatctcat gcatcgtttt cactacgcca tccaccactt
    12481 tgccgccgtg ctgggcggtg tcggaggcac tttgcgccag ttgcgaggcc tggcgggcgt
    12541 tatcggcgtt ttgcttcact gtcgcggtga gctgctccat gctggcggca gtttcttcca
    12601 gcgcggatgc ctgctgttcc gtacgggagg aaagatcggt gttgcccgcc gcaatttcac
    12661 gggtaccggc atagatggca tctgaacctt cacgcacatg agtgacggtg tcagtcaaag
    12721 agcgttgcat atgtgaaacg ctctgcgcca ggtcgcccat ttcactgcgc ccgtcaatgg
    12781 tcagggtatt cgccaggtta ccaccggcga tttcgcgaat gtgagcaata atttttgcca
    12841 gcggtgtaag caacatacgg cgaatgccgt accacgccac cagcagaatc aataccacca
    12901 ccagcgcgat aaccgccagt tgccactggg caaatcggta atcatctgcg ttgtcagtga
    12961 cgatatcgcg atacagtttt tcactgctga gggcgtactg agcaaacgct tcgcccattg
    13021 cattttgcat tccctgggtt ggctgagcga aataagctcc agtattgcca taatcaagat
    13081 aatcaatcag ttcagttaac gctgtgtgat agtttttata tttttcatca atattacgac
    13141 tggtagcgac catttcaggt aacggtgcca tgcttttgaa ttttttataa tgagtcgctg
    13201 cctgggccaa tgttttcctg gcgctatcga gcaattcaac tttggcatta ctttgctgat
    13261 tggatgaatc catcatcatc cgcaccgctg aacgactcag gttaatgcgc gtttgcagca
    13321 ttaaatccca tgttgacgtc agctcgccct gctgttcccg taattgattg gaaaccacaa
    13381 agctcttctg gctatggtga agggaagaaa aaaacagact gccggaaata agctgtaaca
    13441 gtgcgaatac ccccagcacc attaccaaca gcgtgactac gcggatacgg ttaatcataa
    13501 ggcaccttcc tgaaaacaag ttgatctcgt tatcggcaag gaagggggaa actttattgc
    13561 tgatgccacc cgccgcgaaa ctgaaataaa aaacccgatg cgcagatcat cgggttcatt
    13621 gcaattgagg aaatcgggag aattacgcca cttctgacgc cgcgctatct aatagcgcca
    13681 tctcttcgct gttcagcagt ttttcgatgt tcaccagaat caacatacgg tcgcccagtg
    13741 cacccagtcc agtgagatat tctgttgaaa gcgtcacggc aaattccggt gccggacgaa
    13801 tttgctccgc cgtcaatgaa agcacgtctg agacgccgtc aaccacgatg ccgaccaccc
    13861 gctgtccgag attcaggacg ataactaccg tgttgtcgtt atagtccaca tccacctggc
    13921 tgaacttaat tcgtaagtcc acaatcggca caataacacc gcgcagattc gtgacgcctt
    13981 tgataaacgc tggcgtgttc gcaatccgtg ttacctgatc gtagccacgg atctcctgca
    14041 ctttcagaat atcaataccg tactcttcat caccaagggt aaataccaga aattcctggc
    14101 ctgacggctc gctggccagc tttgttacat tcgtcatacc ggtcatattg ttaccttttt
    14161 actcattcag gcggcggtgt tcgccatacg ttgttcgcgg tttatcgcct gcaaggcgga
    14221 gacatcaaca atcagtgcca cgctgccgtc gccaagaatg gtcgcagcag aaatgccggg
    14281 gactttgcga tagttacttt caaggttttt aaccacaacc tggtgttgac caattaattg
    14341 atccaccagc aaggcgtagc ggcgaccgcc actttgtaag atcaccacaa ttccctgggt
    14401 agcttcggtt ttcgcgcccg cgacgttgaa cactttccac agttcgacga tgggcagata
    14461 ttcaccccgc acttccagca cccgctcgcc gccagccagt ggatgcagat cggcttcacg
    14521 gggttgcagt gattccatga cagcattcag cggcagaatg aaaacttcat ccgcaacgcg
    14581 tacggacatg ccgtcgagga tggccagcgt cagcggcagt aaaatgcgga tcgtcgtgcc
    14641 agtaccctgc ttcgactgga tttcgacatg accgcccatc tcctggatat tacgtttaac
    14701 gacgtccatg ccgacgccgc gcccggagac gtcggtgacc tgctccgccg tggagaagcc
    14761 aggggcaaat atcagcatcg cgacttcgtc gtcgctcatg ttttcgctga cggtcagacc
    14821 ttgcgaggcc gcttttgcca gaattcgctc acggtttagc cccgcaccat cgtcggtcac
    14881 ttcaatgcaa atgttgccgc cctgatgttc ggcagacaga attaaatttc cgacgctgtt
    14941 tttacctgcg gcgagccgtt tttctggcag ttcaataccg tgatcgaggc tattgcgtac
    15001 caggtgggtc agcgggtcga taatgcgttc tatcaggctc ttgtcgagtt cagtggaact
    15061 gcccaccagc gtcagttcta cctgcttgcc gagttttccc gcaagatcac gcaccagccg
    15121 gggatagcga ctaaagacat actccatcgg catcatgcga atcgacatca ctgattcctg
    15181 caaatcacgg gcgttacgtt gtaactgccc catgctggta atcaaatcac catgattaac
    15241 cgggtccagt tcactagaac gctgggcaag cattgactgg gtgatgacca gctcgccgac
    15301 gaggttaatt aattgatcaa ccttttctac cgctacacgg atgctggtgg attcactgct
    15361 gcgcgtcgtt ttttcccgct ccacgcggcc ggttggcgct tgttcggctg ccagtttaag
    15421 cactggtggg ggggatattt tgggcgagac ttctactgtt tcaaaggtaa tctgatcggc
    15481 ttcaatcaca aaacagagta ccgctgtgat gtcatcttcg gcgatgtcgc ccggtaatat
    15541 tgccgagaga gaatccgccc ctttcaccac atcagttaac gttgtcagat gccccagctc
    15601 ttcttccagc aggtcgactt ccccggcctt caggcgcgaa aggataattc gtcgcggcga
    15661 ctgatcgcgg ctctgctcat cttgcggttc acttttggca accacactta atcgggtcac
    15721 tgcagatggc gtttcgcctt tcgcttctaa tgccagttga cgcaaggcct ggcagatata
    15781 atcgaagctg gcggcatccg gctcttgcga ctgtttataa gcgtcgagct gttcttgcat
    15841 gatgtccttc gtttccaaaa acagattgat aatgtcggtg ttgagttgca tctcacctcg
    15901 tctggcttcg tcgagcaggt tttccatcag atgcgtggtt tcctgcaaaa cgctgaagcc
    15961 aaaggttcct gcccctcctt tgatcgagtg ggcagcccga aagatggcat tcaattgttc
    16021 ggcatctggc gcttccggct gcaaaaccag cagatgctgc tccatgtcag ccaacagttc
    16081 gtccgcttca tcaaaaaatg tctgataaaa atcgcttata tccatgctca cgctgtcacc
    16141 tcggttcggc tgatggcatt gtgggaacac tgacctgtgg tgcaacctca ggtttttcca
    16201 gggcgcttac tggctcattc tggctttcgg cgttttcatg caaaatggcc tgttcggctt
    16261 gtttgttcag taccagcagg ctgatgcgac ggttgacggc atcatcaggt ccgcgatcgc
    16321 ttaagcgcat cgttgccgcc atgccgacga cacgtaacac tttgccgcca ttcaacccac
    16381 caaccatcag ttcgcggcgg gatgcattgg cacgatcggc agaaagctcc cagttgctgt
    16441 aacctttctc accgctggcg tagggaaaat catcggtatg acccgagagg ctaattcggt
    16501 tgggaatacc gttcagtacg ggcgcgatgg cgcgcagaat gtcgcgcata tagggttcga
    16561 cgtcggcact gccagttcta aacatcgggc gattctggct atcgatgatc tgaatacgta
    16621 gaccttcctg gaccagatcg attttgagat gtggacgtaa cgcccgcagt ttcggatcgg
    16681 actcaatcaa ctggtcgaga tcgccacgca atttccgcaa tcgactttgc tccatgcgtt
    16741 ttttcagctc ttcgatgttc ggctgcttat tcacttcccc ctggctttgg gtgtaatcat
    16801 cacccccacc gggaattggg ctttcactat tagaaatgcg atcgccgccc gtaaccgcag
    16861 tcgccagtgg agtccggaag tactccgcaa tctgaatcag ctcttttggg ctggagatgg
    16921 agattaacca catcaccaga aaaaaggcca tcatcgcggt cataaagtcg gcataagcaa
    16981 tcttccacga tccatgtgct gccccgtggc ttttggcttt gcgtcgtttg acgacaataa
    17041 tcggatgcgc ttgattcttc atgcttcctc ggttgtcgtc tgttgttgcg gatttttcac
    17101 cgcacgcaca tgctcttcca gttcaataaa cgacggacgt tcgctggaat agagcgtttt
    17161 gcgaccaaac tcaacggcga taggcggtgc gtaaccgttc agattagata gcagtgtgac
    17221 tttgacgcac tgcatcattt tgctggtttc ggcgcttttc tgacgtaaaa cactcgctaa
    17281 tggggaaata aatccgtaag ccaataaaat accgaggaaa gtccccacca tcgcatgtgc
    17341 gataagcgcc cccagctcgg cggcaggacg atcggctgaa cctaacgcgt gaacgacccc
    17401 cattacagcc gcaacaatac caaacgccgg aagtgagtca ccgaccagcg ccagactgtt
    17461 cgccgggact tctgcctcgc tttcgtgcgt ctcaatctct tcatccatca gagcttcgat
    17521 ttcgaaggtg ttcatgtgac cgctgataat caggcgcaga taatcgacga taaattcaag
    17581 catgacgcta tccgcaagga tgcgtgggta gctggcgaag atctcgctct cacggggatt
    17641 ttcaatatca cgttccagcg aaaacatccc catctgccgc gatttcgcca tcaaccgata
    17701 aagcagagcc agcagatcca tatacattgc tttggtgtat ttggagcgac gaaacagcaa
    17761 cggcagcgcc ttcagcgtgc ctttaatcgc tttgccatta ttgccgacga taaacgaccc
    17821 aatccctgca ccggcaataa tcaccagttc agcgggttga tagagtgctc caaggcttcc
    17881 accggtcatc aaataaccgc cgaaaactgt accgagaaca accaggtaac ctaataagat
    17941 aagcacgaca tcatccttcc actgttgacc atgacaggat gttcagtcgt caggcgttaa
    18001 cacgcgatag gggcaaaaaa aagcagcggt acgtcgttac cgctgctgga atgtttcgcc
    18061 acaccgtatc agttaaacag cctgtactct ctgttcatcc agcagttgtg ggataatatc
    18121 ggcaggattc tgggaaagtt tacgtctttt tactgcccgg gatggcggct gacataagct
    18181 gcaggcaaag ctgccaacag gctggtgagc gtgggtaata aaattgccgc cgcagcagtt
    18241 gcagctggaa agttgcagta atccactttc aacaaaccgc acgaatgtcc aggcacgggt
    18301 taatgccagc agcggtccct cttctgcttg cgggcactgt tcaaggtata aacggtaggc
    18361 tttgatcacc gcatcgacgc cattacacaa accggttttc agtaaaaact gccatgcgtt
    18421 acagaacatc gaagcatgaa cgttttgttc ccaggtcata aaccagtcgg ttgagaatgg
    18481 cagcatgcct ttcggcggtg ggcttccgcg cagttcttta tagagtttta tcaggcgtcc
    18541 gcgacttaac tgtgtttcgc tttccagcat ctgcaaacga gcgcccaggg tgatcaattc
    18601 cattgccagc tgaatatccc gcgcttcctg aacaatgctt ttttcactca tgatcaggcc
    18661 cttttcttgc gcagcgcttc ttcaggctga ttaacatcat tcagcaagcg tgttgagagc
    18721 atgatgccgg tatgaatttg ctggagatcg tcaacgcggg aatcttgcgt caactgagta
    18781 atcgtctggt gactgtcaaa acggaagtga caaaccagtt gattggtttc tgccagctta
    18841 accatttgcg gaagagtcag tgccgctaac gttgtcgcca tttcttcatt tatgccgaga
    18901 cgaaacatag cggacgcttt gtcctgaaca atcagacgct gtgcaagtag taaatatgac
    18961 aagttgatgt cataaatgtg tttcagcaac tcggaggtat gcattattcc cacccagaat
    19021 aaccaacttt atttttatgc ggtctcaccg caccccgtga tgtcgccggg aagccccggt
    19081 aaaaaataat tagcattaga atagttgcga taagctgcaa taggcagagc ccctttgtgg
    19141 gatttaataa gtccttacaa atagaagggg ttctttacac ttatctaaga tttttcctaa
    19201 atcgacgcaa ctttactcgt cactacacgc acatacaacg gaggggggct gcgattttca
    19261 ataatgtgtg atgcagatca cacaaaacg
//
LOCUS       DAZFLD010000086        19086 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.086, whole genome shotgun sequence.
ACCESSION   DAZFLD010000086 DAZFLD010000000
VERSION     DAZFLD010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19086
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.086"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(91..2757)
                     /gene="cas3"
                     /locus_tag="ACHKBL_002545"
     CDS             complement(91..2757)
                     /gene="cas3"
                     /locus_tag="ACHKBL_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase/endonuclease Cas3"
                     /protein_id="HFO5819398.1"
                     /translation="MEPFKYICHYWGKSSKSLTKGNDIHLLIYHCLDVAAVADCWWDQ
                     SVVLQNAFCRNEMLSKQKVKAWLLFFIALHDIGKFDIRFQYKSAESWLKLNPATPSLN
                     GPSTQMCRKFNHGAAGLYWFNQDSLSEQSPGDFFSFFDAAPHPYESWFPWVEAVTGHH
                     GFILHSQDQDKSRWEMPASLASYAAQDKQAREEWISVLEALFLTPAGLSINDIPPDCS
                     SLLAGFCSLADWLGSWTTTNTFLFKEDAPSNINALRTYFQDRQQDASRVLELSGLVSN
                     KRCYEGVHALLDNGYQPRQLQVLVDALPVAPGLTVIEAPTGSGKTETALAYAWKLIDQ
                     QLADSVIFALPTQATANAMLSRMEANASRLFTSPNLILAHGNSRFNHLFQSIKSRAFT
                     EQGQEEAWVQCCQWLSQSNKKVFLGQIGVCTIDQVLISVLPVKHRFIRGLGIGRSVLI
                     VDEVHAYDTYMNGLLEAVLKAQADVGGSVILLSATLPMKQKQKLLDTYGLHTDPEENN
                     SAYPLINWRGVNGAQRFDLLAHSEQLPPRFSIRPEPIYLADMLPDLRMLERMIVAANA
                     GAQVCLICNLVDVAQLCHQRLKELNNTQVDIDLFHARFTLNDRREKENRVISNFGKNG
                     KRNVGRILVATQVVEQSLDVDFDWLITQHCPADLLFQRLGRLHRHHRKYRPAGFEVPL
                     ATVLLPDGEGYGRHEHIYSNVRVMWRTQQHIEELNGASLFFPDAYRQWLDSIYDDAEM
                     DEPEWVIKGMDKFESAECEKRFKARKVLQWAEEYSLQDNDETILAVTRDGEMSLPLLP
                     YLQTSSGKQLLDGQVYEDLSHEQQYEALALNRVNVPFTWKRNFSEVVDEDGLLWLEGK
                     QNQDGWIWQGNNIVITYTRDEGMTRVIPANPK"
     gene            complement(3116..3850)
                     /gene="cysH"
                     /locus_tag="ACHKBL_002546"
     CDS             complement(3116..3850)
                     /gene="cysH"
                     /locus_tag="ACHKBL_002546"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HFO5819399.1"
                     /translation="MSKLDLNALNELPKVDRILALAETNAELEKLDAEGRVAWALDNL
                     PGEYVLSSSFGIQAAVSLHLVNQIRPDIPVILTDTGYLFPETYRFIDELTDKLKLNLK
                     VYRATESAAWQEARYGKLWEQGVEGIEKYNEINKVEPMNRALKELNAQTWFAGLRREQ
                     SGSRANLPVLAIQRGVFKVLPIIDWDNRTIYQYLQKHGLKYHPLWDEGYLSVGDTHTT
                     RKWEPGMAEEETRFFGLKRECGLHEG"
     gene            complement(3924..5636)
                     /gene="cysI"
                     /locus_tag="ACHKBL_002547"
     CDS             complement(3924..5636)
                     /gene="cysI"
                     /locus_tag="ACHKBL_002547"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009337 - sulfite reductase complex
                     (NADPH) [Evidence IEA];
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HFO5819400.1"
                     /translation="MSEKHPGPLVVEGKLTDAERMKLESNYLRGTIAEDLNDGLTGGF
                     KGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPRHAMLLRCRLPGGVITTKQWQAIDKF
                     AGENTIYGSIRLTNRQTFQFHGILKKNVKPVHQMLHSVGLDALATANDMNRNVLCTSN
                     PYESQLHAEAYEWAKKISEHLLPRTRAYAEIWLDQEKVATTDEEPILGQTYLPRKFKT
                     TVVIPPQNDIDLHANDMNFVAIAENGKLVGFNLLVGGGLSIEHGNKKTYARTASEFGY
                     LPLEHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVETFKAEVERRAGIKFEPI
                     RPYEFTGRGDRIGWVKGIDDNWHLTLFIENGRILDYPGRPLKTGLLEIAKIHKGDFRI
                     TANQNLIIAGVPESEKAKIEKIAKESGLMNAVTPQRENSMACVSFPTCPLAMAEAERF
                     LPSFIDNIDNLMAKHGVSDEHIVMRVTGCPNGCGRAMLAEVGLVGKAPGRYNLHLGGN
                     RIGTRIPRMYKENITEPEILASLDELIGRWAKEREAGEGFGDFTVRAGIIRPVLDPAR
                     DLWD"
     gene            complement(5636..7435)
                     /gene="cysJ"
                     /locus_tag="ACHKBL_002548"
     CDS             complement(5636..7435)
                     /gene="cysJ"
                     /locus_tag="ACHKBL_002548"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA];
                     GO_process: GO:0019344 - cysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     flavoprotein subunit"
                     /protein_id="HFO5819401.1"
                     /translation="MTTQVPPSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVL
                     NQQPAALAATPAPAAEMPGITIISASQTGNARRVAEALRDDLLAAKLNVKLVNAGDYK
                     FKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
                     FCQSGKDFDSKLAELGGERLLDRVDADVEYQAAAIEWRARVVDALKSRAPVAAPSQSV
                     ATGAVNEIHTSPYSKDAPLVASLSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDAL
                     GVWYQNDPALVKELVELLWLKGDEPVTVDGKTLTLSEALQWHFELTVNTANIVENYAT
                     LTRSETLLPLVGDKAKLQHYAATTPIVDMVRFSPAQLDAEALINLLRPLTPRLYSIAS
                     SQAEVENEVHVTVGVVRYDVEGRARAGGASSFLADRVEEDGEVRVFIEHNDNFRLPAN
                     PETSVIMIGPGTGIAPFRAFMQQRAADEAPGKNWLFFGNPHFTEDFLYQVEWQRYVKE
                     GVLTRIDLAWSRDQKEKIYVQDKLREQGAELWRWINDGAHIYVCGDANRMAKDVEQAL
                     LEVIAEFGGMDTEAADEFLSELRVERRYQRDVY"
     gene            7754..8116
                     /gene="queD"
                     /locus_tag="ACHKBL_002549"
     CDS             7754..8116
                     /gene="queD"
                     /locus_tag="ACHKBL_002549"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HFO5819402.1"
                     /translation="MSTTLFKDFTFEAAHRLPHVPEGHKCGRLHGHSFMVRLEITGEV
                     DPHTGWIIDFAELKAAFKPTYERLDHYYLNDIPGLENPTSEVLAKWIWDQVKPVVPLL
                     SAVMVKETCTAGCIYRGE"
     gene            8194..9465
                     /gene="ygcN"
                     /locus_tag="ACHKBL_002550"
     CDS             8194..9465
                     /gene="ygcN"
                     /locus_tag="ACHKBL_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFO5819403.1"
                     /translation="MEDDCDIIIIGAGIAGTACALRCARAGLSVLLLERAEIPGSKNL
                     SGGRLYTHALAELLPQFHLTAPLERRITHESLSLLTPDGATTFSSLQPGGESWSVLRA
                     RFDPWLVAEAEKEGVECIPGATVDALYEENGRVCGVICGDDILRARYVVLAEGANSVL
                     AERHGLVTRPAGEAMALGIKEVLSLETSAIEERFHLENNEGAALLFSGGICDDLPGGA
                     FLYTNQQTLSLGIVCPLSSLTQSRVPASELLARFKTHPAVRPLIKNTESLEYGAHLVP
                     EGGLHSMPVQYAGNGWLLLGDALRSCVNTGISVRGMDMALTGTQAAAQTLISACQHRE
                     PQNLFALYHHNVERSLLWDVLQRYQHVPALLQRPGWYRAWPALMQDISRDLWDQGDKP
                     VPPLRQLFWRHLRRHGLWHLAGDVIRSLRCL"
     gene            9456..9716
                     /gene="ygcO"
                     /locus_tag="ACHKBL_002551"
     CDS             9456..9716
                     /gene="ygcO"
                     /locus_tag="ACHKBL_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HFO5819404.1"
                     /translation="MSVTRNLWRVADAPHIVPADSVERQTVQRLINACPAGLFSLTPE
                     GDLRVDYRGCLECGTCRLLCDEKTLQQWRYPPSGFGITYRFG"
     gene            9733..10308
                     /gene="ygcP"
                     /locus_tag="ACHKBL_002552"
     CDS             9733..10308
                     /gene="ygcP"
                     /locus_tag="ACHKBL_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
                     /protein_id="HFO5819405.1"
                     /translation="MPLLHLLRQNPVIAAVKDNASLQLAIDSECQFISVLYGNICTIS
                     QIVKKIKNAGKYAFIHVDLLEGASNKEVVIQFLKLVTEADGIISTKASMLKAARAEGF
                     FCIHRLFIVDSISFHNIDKQVAQSNPDCIEILPGCMPKVLGWVTEKIRQPLIAGGLVC
                     DEEDARNAINAGVVALSTTNTGVWTLAKKLL"
     gene            complement(10455..11315)
                     /gene="ygcQ"
                     /locus_tag="ACHKBL_002553"
     CDS             complement(10455..11315)
                     /gene="ygcQ"
                     /locus_tag="ACHKBL_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HFO5819406.1"
                     /translation="MNIAIVTINQEHAAMAGWLAAQDFSGSTLTHWQIEPQPMVAEQV
                     LDALVEQWQRTPAEVVLFPPGAFGDELSIRLAWRLHGASICQVTSLDIPTVSVRKSHW
                     GNALTATLQTEKRPLCLSLARQAGAEINTMLPSGMLQLNIVPGALPDWLISIENLKNV
                     TRDPLAEARRVLVVGQGGEADNQEIAMLVEKLGAEVGYSRARVMNGGVDAEKVIGISG
                     HLLAPEVCIVVGASGAAALMAGVRNSKFVVAINHDASAAVFSQADVGVVDDWKAVLEA
                     LVTTIHADCQ"
     gene            complement(11312..12091)
                     /gene="ygcR"
                     /locus_tag="ACHKBL_002554"
     CDS             complement(11312..12091)
                     /gene="ygcR"
                     /locus_tag="ACHKBL_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HFO5819407.1"
                     /translation="MNILLAFKAEPDAGMLAEKEWQAAAQGNIGPDVSLLRSLLGADE
                     QAAAALLLAQRKNGTPMSLTALSMGDERALHWLRYLMALGFEDAVLLETAADLRFAPE
                     FVARHIAEWQRQNPLDLIITGCQSSEGQNGQTPFLLAEMLGWPCFTQVERFTLDAPFI
                     TLEQRTEHGLRCCRVRLPAVIAVRQCGEVALPVPGMRQRMAAGKAEITRETVAAEAPA
                     IQCLQLARPEQRRRAALIDGQTVAEKAQKLWQDYLRQRMQP"
     gene            complement(12069..13406)
                     /gene="ygcS"
                     /locus_tag="ACHKBL_002555"
     CDS             complement(12069..13406)
                     /gene="ygcS"
                     /locus_tag="ACHKBL_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO5819408.1"
                     /translation="MNTSPVRMDDLPLNRFHCRIAALTFGAHLTDGYVLGVIGYAIIQ
                     LTPAMQLTPFMAGMIGGSALLGLFIGSLVLGWISDHIGRQKIFTFSFLLITLASFLQF
                     FATTPEHLIGLRILIGIGLGGDYSVGHTLLAEFSPRRHRGVLLGAFSVVWTVGYVLAS
                     IAGHHFISESPEAWRWLLASAALPALLITLLRWGTPESPRWLLRQGRFAEAHAIVHRY
                     FGPHVLLGDEVATATHKHIKTLFSSRYWRRTAFNSVFFVCLVIPWFVIYTWLPTIAQT
                     IGLEDALTASLMLNALLIVGALLGLVLTHLLAHRRFLLGSFLLLAATLVVMACLPSGS
                     SLTLLLFVLFSTTISAVSNLVGILPAESFPTDIRSLGVGFATAMSRLGAAVSTGLLPW
                     VLAQWGMQATLLLLAAVLLVGFVVTWLWAPETKALPLVAAGNVGGANEHSVSV"
     gene            complement(13499..14953)
                     /gene="ygcU"
                     /locus_tag="ACHKBL_002556"
     CDS             complement(13499..14953)
                     /gene="ygcU"
                     /locus_tag="ACHKBL_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HFO5819409.1"
                     /translation="MSLSRAAIVDQLKEIVGADRVITDETVLKKNSIDRFRKFPDIHG
                     IYTLPIPAAVVKLGSTEQVSRVLNFMNAHKINGVPRTGASATEGGLETVVENSVVLDG
                     SAMNQIINIDIENMQVTAQCGVPLEVLENTLREKGYTTGHSPQSKPLAQMGGLVATRS
                     IGQFSTLYGAIEDMVVGLEAVLADGTVTRIKNVPRRAAGPDIRHIIIGNEGALCYITE
                     VTVKIFKFTPENNLFYGYVLEDMKTGFNILREIMVEGYRPSIARLYDAEDGTQHFTHF
                     ADGKCVLIFMAEGNPRIAKATGEGIAEIVARYPQCQRVDSKLIETWFNHLNWGPDKVA
                     AERVQILKTGNMGFTTEVSGCWSCIHEIYESVINRIRTEFPHADDITMLGGHSSHSYQ
                     NGTNMYFVYDYNVVDCKPEEEIDKYHNPLNKIICEETIRLGGSMVHHHGIGKHRVHWS
                     KLEHGSAWALLEGLKKQFDPNGIMNTGTIYPIEK"
     gene            complement(15023..15808)
                     /gene="ygcW"
                     /locus_tag="ACHKBL_002557"
     CDS             complement(15023..15808)
                     /gene="ygcW"
                     /locus_tag="ACHKBL_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFO5819410.1"
                     /translation="MSIESLNAFSMDFFSLKGKTAIVTGGNSGLGQAFAMALAKAGAN
                     IFIPSFVKDNGKTKEMIEKQGVEMDFMQVDITAEGAPQKIIAACCERFGTVDILVNNA
                     GICKLNKVLDFGRADWDPMIDVNLTAAFELSYEAAKIMIPQKSGKIINICSLFSYLGG
                     QWSPAYSATKHALAGFTKAYCDELGQYNIQVNGIAPGYYATDITLATRSNPETNQRVL
                     DHIPANRWGDTQDLMGAAVFLASQASNYVNGHLLVVDGGYLVR"
     gene            16126..17403
                     /gene="yqcE"
                     /locus_tag="ACHKBL_002558"
     CDS             16126..17403
                     /gene="yqcE"
                     /locus_tag="ACHKBL_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter"
                     /protein_id="HFO5819411.1"
                     /translation="MQHNSYRRWITLAIISFSGGVSFDLAYLRYIYQIPMAKFMGFSN
                     TEIGLIMSTFGIAAIILYAPSGVIADKFSHRKMITSAMIITGLLGLLMATYPPLWVML
                     CIQVAFAITTILMLWSVSIKAASLLGDHSEQGKIMGWMEGLRGVGVMSLAVFTMWVFS
                     RFAPDDSASLKTVIIIYSVVYILLGILCWFFVSDNNNLRSTNNEEKQSFQLSDILAVL
                     RISTTWYCSMVIFGVFTIYAILSYSTNYLTEMYGMSLVAASYMGIVINKIFRALCGPL
                     GGIITTYSKVKSPTRVIQILSVLGLLALTALLVTNSNPQSVAMGIGLILLLGFTCYAS
                     RGLYWACPGEARTPSYIMGTTVGICSVIGFLPDVFVYPIIGHWQDTLPAAEAYRNMWL
                     MGMAALGMVIVFTFLLFQKIRTADSAPAMASSK"
     gene            17430..18908
                     /gene="ygcE"
                     /locus_tag="ACHKBL_002559"
     CDS             17430..18908
                     /gene="ygcE"
                     /locus_tag="ACHKBL_002559"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HFO5819412.1"
                     /translation="MSKKYIIGIDGGSQSTKVVMYDLEGNVVCEGKGLLQPMHTPDAD
                     TAEHPDDDLWTSLCFAGHDLMSQFAGNKEDIVGIGLGSIRCCRALLKADGTPAAPLIS
                     WQDARVTRPYDHTNPDVAYVTSFSGYLTHRLTGEFKDNIANYFGQWPVDYKSWTWSED
                     AAVMDKFNIPRHMLFDVQMPGTILGHITPQAALATHFPAGLSVVCTTSDKPVEALGAG
                     LLDDETAVISLGTYIALMMNGKALPKDPVAYWPIMSSIPQTLLYEGYGIRKGMWTVSW
                     LRDMLGESLIQDARAQDLSPEDLLNKKASSVPPGCNGLMTVLDWLTNPWEPYKRGIMI
                     GFDSSMDYAWIYRSILESVALTLKNNYDNMCNEMNHFAKHVIITGGGSNSDLFMQIFA
                     DVFNLPARRNAINGCASLGAAINTAVGLGLYPDYATAVDKMIRVKDIFIPIESNAKRY
                     DAMNKGIFKDLTKHTDVILKKSYEVMHGELGNADSIQSWSNA"
BASE COUNT         4626 a         4950 c         4702 g         4808 t
ORIGIN      
        1 agaagatgta cattgtgcac tttccctact taagtaggga taaaccgtta ttggtcttgt
       61 tatcgtcatt gataccaatc attcccgaag ttatttggga tttgcaggga tgactctggt
      121 catcccttca tcccgagtgt aggtaataac aatattatta ccctgccaga tccatccatc
      181 ctgattctgt ttcccttcca gccaaagtaa cccatcttca tctactactt cagaaaaatt
      241 acgtttccag gtgaagggta cattgacgcg attaagtgct aacgcctcat actgctgttc
      301 atgacttagg tcctcgtaga cctggccatc gagcagttgt ttacctgaag acgtttgtaa
      361 ataaggcaat aatggcaggc tcatttcccc atccctcgtt accgcaagaa ttgtttcatc
      421 gttatcctgc aagctatatt cttcagccca ctgcagaacc ttgcgagcct tgaacctctt
      481 ttcacactca gcgctttcaa acttatccat gcctttgatg acccattctg gctcatccat
      541 ttcagcatca tcgtaaatgc tatccagcca ttgccggtag gcatcgggga aaaataagga
      601 tgctccatta agttcctcaa tatgttgctg cgtccgccac atgactctaa cgttgctata
      661 aatatgctca tgtcgtccgt aaccttcgcc atcaggcagc aaaacggtgg caagaggaac
      721 ctcaaaacca gcgggacgat atttgcgatg atggcgatgt aaacggccca atcgttggaa
      781 aagtaaatct gcggggcaat gctgagtaat taaccaatca aaatcaacgt cgagtgattg
      841 ttccacgacc tgggtggcga caagtatccg tccaacattt cgcttcccat ttttgccgaa
      901 attgctaata actcgattct ctttttcacg acgatcgttc agcgtaaagc gcgcatgaaa
      961 caaatctata tctacttgcg tgttatttag ttcctttaac cgttggtggc atagctgtgc
     1021 aacatcaacc aaattgcaga taagacagac ttgtgcaccc gcatttgccg ctacgatcat
     1081 tcgctctaac atcctaaggt caggtaacat gtcagctaaa taaataggtt ctggccgaat
     1141 cgaaaagcgg ggcgggagtt gttctgaatg agctaacaga tcaaaacgtt gtgcaccatt
     1201 cacacctcgc cagttaatga gtggatatgc ggagttattt tcctctggat ctgtatgcag
     1261 accataagta tccagaagtt tctgtttttg tttcattggt agagttgcgg aaagaagaat
     1321 aacactcccc cccacatcag cctgagcctt gagcacagcc tccagcaagc cgttcatata
     1381 cgtgtcgtaa gcatgaactt catcaacaat taaaacactt cgaccaattc ccaaaccacg
     1441 gataaagcgg tgtttaactg gcaataccga tatcaacacc tgatcaatcg tgcaaacgcc
     1501 gatttgccca agaaacactt tcttattgct ttgtgacaac cactgacaac actgaaccca
     1561 tgcttcttct tgcccctgct cagtaaatgc gcgtgatttt attgattgaa aaaggtggtt
     1621 aaaacgtgaa ttaccatgag caagaataag atttggggag gtaaataaac ggctcgcgtt
     1681 cgcttccatt ctgctgagca ttgcatttgc agtagcttgt gtagggaggg caaaaataac
     1741 actatccgca agttgttggt caataagttt ccaggcatag gccagcgcag tttcagtttt
     1801 acctgaaccc gtaggtgcct ctattaccgt cagcccgggc gctactggaa gagcatcaac
     1861 taacacttgt aattgtctgg gttgatagcc attgtccagt agagcatgaa caccttcata
     1921 acatcgctta tttgatacaa ggccgctcaa ctccaatacc cggctcgcat cctgctgtcg
     1981 gtcctggaaa tacgttctta gagcatttat gttggaaggt gcatcctctt taaacagaaa
     2041 ggtattcgtt gtagtccagg agcctaacca gtcagcaagc gagcaaaaac ctgctaacag
     2101 tgatgaacaa tcaggtggta tatcgtttat agataacccg gctggcgtta aaaataatgc
     2161 ttcgagtaca gatatccact cctcacgagc ctgtttatct tgcgcagcat aagatgccag
     2221 agaagctggc atttcccaac gcgacttatc ttgatcctgg gaatgtaata taaaaccatg
     2281 atgtcctgta acagcctcta cccatggaaa ccaggactca taaggatgag gagcggcatc
     2341 aaaaaaactg aaaaaatccc cgggagattg ctctgaaagt gaatcctggt taaaccaata
     2401 cagaccggct gcaccatgat taaatttacg gcacatttgt gttgatggac cattaagtga
     2461 tggcgttgca ggatttaatt tcagccaact ttctgctgat ttatattgga atcgtatatc
     2521 aaactttccg atatcatgaa gggcaatgaa aaataacagc caggctttca ccttctgttt
     2581 tgatagcatt tcatttcggc aaaaagcatt ttgtagtacg actgattgat cccaccagca
     2641 atctgcaaca gctgcaacat ccaggcaatg ataaatcaac agatgaatat catttccttt
     2701 cgtcaggctt tttgaggatt tcccccagta atgacatata tatttaaaag gttccattaa
     2761 tagcctccct gtttttttag tattatcgat gatatcagtg gactattttt cgatgctgct
     2821 cataaatatc gaattacagt gatatacaca tgtcttgcct ccctgcaaag aagagactaa
     2881 cattcgaatt gcttaggttc gaacgctggc ctcaggttga tagaaatatc gcctggggct
     2941 tttgtccatc tggaacctcg cgaatgctta acgccagaca gcctcaagca cccaactcca
     3001 ttctatacct gttaattctc cctgcgatgt tcacctttaa atgccggaat gctgctcgca
     3061 aaataaaaac gcccccatca tttctgacag aggcgtttaa tttatccggc aatatttacc
     3121 cttcgtgtaa cccacattcc ctttttaagc caaagaaacg cgtttcttct tccgccatgc
     3181 cgggttccca tttacgggtt gtatgggtat caccgaccga taaatatcct tcgtcccata
     3241 atgggtgata tttcaggcca tgtttttgca ggtactgata aatagttcgg ttatcccagt
     3301 cgataatcgg cagcacttta aatacgccac gctgaattgc cagcaccggt aaattggcgc
     3361 ggctgccgga ttgttcacgg cgcaggccag caaaccaggt ttgcgcattc agttctttca
     3421 gcgcccggtt catcggttcg actttgttga tctcgttgta cttctcaatc ccctcaacac
     3481 cctgctccca cagtttaccg tagcgcgctt cctgccaggc ggcgctttcg gtagcgcggt
     3541 acactttcag gttgagcttg agtttgtccg ttaactcgtc aataaagcgg taggtttctg
     3601 ggaacagata gccggtatcg gtgaggatca ccggaatatc cgggcgaatc tgattcacca
     3661 gatgcaggct caccgccgcc tgaatgccaa agctggaaga aagtacatat tcaccgggca
     3721 gattatccag cgcccaggca acgcggcctt cagcgtccag tttttccagt tcggcgttag
     3781 tttccgccag cgccagaatg cgatctacct tcggcagttc gttcagggcg tttagatcga
     3841 gtttggacat agattcctca ctgtttgcct tgcctgatgc gacgctcacg cgtcttatca
     3901 ggcctacaag accgggctaa tggttaatcc cacaaatcgc gcgccggatc gagcaccggg
     3961 cgaatgatgc ccgcacgcac cgtaaagtcg ccgaagcctt cacccgcttc gcgctctttc
     4021 gcccagcgcc ctatcagttc atcaagcgac gccaggattt ccggctcggt gatgttttct
     4081 ttatacatcc gtgggatacg tgtcccaatg cggttgccgc caagatgcaa gttgtagcga
     4141 cctggcgctt tacccaccag gcccacttcc gccagcatcg cgcgaccaca accgttcggg
     4201 cagcctgtta cacgcatcac gatatgctca tcgctgacac catgtttcgc cattaaatta
     4261 tcgatgttgt cgataaaaga cggcaggaaa cgctcggctt cggccatcgc cagcgggcaa
     4321 gtcgggaatg acacgcaggc cattgagttt tcacgctgcg gcgtgacggc attcattaac
     4381 ccgctctctt tggcgatctt ctcgatcttc gctttctcgc tttccggcac accggcgatg
     4441 atcagattct ggttcgccgt aatgcggaaa tcgcctttgt ggatcttcgc gatctccagc
     4501 aggccggttt tcagcggacg cccaggataa tcaaggatgc gaccattttc gataaacagc
     4561 gtcagatgcc agttatcatc aatgccctta acccagccaa tacgatcgcc gcgtccggta
     4621 aactcatacg gacggatcgg ttcaaatttg atccccgcgc gacgctccac ttccgcttta
     4681 aacgtctcaa cccccacgcg ctccagcgtg tatttggttt tggcattttt acgatcggtt
     4741 cggttacccc agtcacgctg agttgtcacg acggcttccg ccactgccaa cgtatgctcc
     4801 agcggcagat agccaaactc actcgcggtg cgggcgtagg ttttcttgtt gccgtgttcg
     4861 atggaaagcc caccgcccac caacaggtta aagcccacca gcttgccgtt ttcggcgatt
     4921 gccacgaagt tcatgtcgtt ggcgtgcaga tcgatatcgt tctgcggcgg gatcactacc
     4981 gtggttttga acttacgcgg caggtaggtc tgaccaagga tcggctcttc atcagtagtg
     5041 gcgacttttt cctgatcgag ccagatctcc gcatacgcgc gggtacgagg cagcagatgt
     5101 tcagagattt tcttcgccca ttcatatgct tccgcgtgca gttgcgactc gtaagggttc
     5161 gaggtgcaga gtacgttacg gttcatgtcg ttagcagtcg ccagcgcatc aaggccgacc
     5221 gagtgcagca tctggtgcac cggtttgacg ttctttttca gaatgccgtg gaactgaaac
     5281 gtctggcggt tggtcaggcg aatgctgcca tagatggtgt tttcaccggc aaatttgtct
     5341 atcgcctgcc actgtttggt ggtgatcacg ccacccggca gacggcagcg caacagcatc
     5401 gcgtggcgcg gctccagctt ctgttcagca cgttcggcgc ggatgtcgcg gtcatcctgc
     5461 tgatacatgc cgtggaagcg gatcagcagg aagttgtcgc ccttaaagcc gccggtcaga
     5521 ccgtcgttta aatcttccgc aatggtgccg cgcaggtagt tgctttcaag cttcatgcgc
     5581 tcggcgtctg tcagttttcc ttcgaccact aaaggccctg gatgtttttc gctcattagt
     5641 agacatctcg ctgataacgg cgctctacgc gcagctcact taaaaattca tccgccgctt
     5701 cggtgtccat gccaccaaat tcggcaatca cttccagaag tgcctgctca acgtctttcg
     5761 ccatgcgatt agcgtcgccg cagacataaa tgtgggcacc gtcattgatc cagcgccaca
     5821 gctccgcgcc ctgttcgcgc agtttgtctt gtacgtaaat tttttctttt tgatcgcgcg
     5881 accaggcaag atcgatacgc gtcagcacgc cctctttgac gtagcgctgc cattccacct
     5941 ggtagaggaa atcttccgta aagtgcgggt tgccaaagaa cagccagttt ttccccggcg
     6001 cctcgtcggc ggcgcgttgc tgcataaagg cgcggaacgg cgcgatgccg gtgcctgggc
     6061 caatcataat caccgacgtt tccggattgg ctggcaggcg gaagttgtcg ttatgttcga
     6121 taaagacgcg gacttcacca tcttcttcca cacgatctgc gaggaagctg gaggcaccac
     6181 cggcacgggc gcggccttcc acgtcgtaac gcaccacacc aacggtaacg tgcacttcgt
     6241 tctcgacttc cgcctgcgag gaggcgatgg aatacaggcg cggggtcagc gggcgcagca
     6301 ggttaatcag cgcttcggca tccagttgcg ccggagagaa acgcaccatg tcaacaatcg
     6361 gcgtggtcgc ggcgtaatgc tgtaacttcg ctttatcgcc caccagcggc agcagcgttt
     6421 cgctgcgggt aagcgtggcg tagttctcaa caatattcgc ggtgttgacg gtcagttcaa
     6481 agtgccactg tagcgcttcg ctcagggtca gcgttttgcc gtcaacggtg acaggttcat
     6541 cgcctttcag ccacagcagt tcgacaagtt ctttcaccag tgccggatcg ttctgatacc
     6601 agacgcccag cgcgtcaccc ggctggtaac gcaggccaga gtcacctaag tcaatttcga
     6661 tatggcgaac gtctttttca gagttacgcc cggtaatttt ctggttaaca gagaggctcg
     6721 ccaccagcgg cgcgtctttg ctgtacgggc tggtgtggat ttcatttacc gcgccagtag
     6781 cgacggattg cgaaggtgcc gcgacaggcg cacgcgattt aagcgcatca accacgcggg
     6841 cgcgccactc gatggcagca gcctggtatt caacgtcggc atcgacacgg tcgagcaggc
     6901 gttcaccgcc cagttccgcc agcttactgt caaaatcttt cccggactgg cagaaaaatt
     6961 cataagaact atcgccgagg ctaaacacgg caaaagcggt attttccagc ttcggcgctt
     7021 ttttggagaa caggaactta tgcagcgcga cggcttcttc cggcggctcc ccttcccctt
     7081 gcgttgacgt cactacgatg agcagttttt cgctggcgat ttgtttgaat ttatagtcgc
     7141 ccgcgttcac cagcttaacg ttcagttttg ctgctaataa atcatcgcgt aatgcttcag
     7201 caacccggcg cgcattgccg gtttgcgagg cggagataat agttataccc ggcatttctg
     7261 cggctggcgc tggcgtcgct gcaagcgcag caggctgctg attgagtacg ccccagaaat
     7321 agccagaaac ccaggcaagc tgggtaggag ttaaatcggt cgtggccgcc tgaaggcgtg
     7381 ccagttgctc cgggttcaac ggaagcaacg cggaaggtgg gacctgtgtc gtcatgcgtc
     7441 gttatgttcc agtaagcaaa gctgtttctg cgccctgtca gcgcccataa aacagaagag
     7501 gaggtaaggt taacggggtg aacggtgtgg attaaagacg ggatagcgat aactaataac
     7561 caaatcgact aacctgtttt agcaatagtc tttaacaaca aaacagatta accaacccaa
     7621 tgaaaaacaa atgaatttag ccaatcatta agataaatca gcgattttgc gcaacaaagt
     7681 cgttttagat aatgcgaaaa aacagccttt ccggtactct acggcggttt tatcgtcctg
     7741 tagagaaatt atgatgtcca ccacgttatt taaagatttc accttcgaag ccgctcaccg
     7801 cttaccacac gtcccggaag ggcataaatg tggtcgcctg cacgggcatt cctttatggt
     7861 gcgactggaa attaccgggg aagtcgatcc gcatacgggc tggattatcg atttcgctga
     7921 actgaaagcg gcgtttaagc caacctacga gcgcctcgat cactattatc tcaatgatat
     7981 tccaggtctg gaaaacccga ccagcgaggt tttagcaaaa tggatttggg atcaagttaa
     8041 acccgttgtg ccgctgttaa gtgcggtgat ggtaaaagaa acctgcaccg caggttgcat
     8101 ctatcgcggc gaatgataag agtgtgtcgg cggtcaattt cccttaagta acgctatgtt
     8161 agggtgttgt gttctggata tctggggcat gacatggaag acgactgcga cattattatt
     8221 attggtgccg gtattgcagg caccgcttgc gcgttacgct gcgcgcgagc gggtttatcc
     8281 gttttgttac tggaacgcgc tgaaatcccc ggcagcaaaa atctttccgg cgggcggtta
     8341 tatacccatg cactcgcgga actcctccct cagtttcacc tgaccgcgcc tcttgaacga
     8401 cgcatcactc acgaaagcct ttccctgtta acgccggatg gcgcaacgac gttttccagc
     8461 ttacagcccg gcggtgaatc ctggagtgta ttacgtgcac ggttcgatcc gtggctggtt
     8521 gccgaagccg aaaaagaagg tgtcgaatgc atccccggtg cgacggtgga tgcgctgtat
     8581 gaagaaaacg gcagggtgtg tggtgtcatt tgtggtgacg atattctccg cgcccgttat
     8641 gtggtgctgg cagaaggtgc caacagcgtc ctggctgaac gtcacgggtt agtgactcgt
     8701 cctgctggcg aagcgatggc gttggggatc aaagaagtgc tgtcgctgga aacatccgct
     8761 attgaagaac gttttcatct ggagaataac gaaggcgcag cgttgctgtt cagcggcggg
     8821 atctgtgatg acttacccgg cggcgcattt ctttatacta atcaacaaac gctctcgtta
     8881 gggattgttt gcccgctctc ttcccttacg caaagtcgtg ttccggcaag cgagctgctg
     8941 gctcgcttta aaacgcatcc ggcagtgcgc ccgcttatca aaaacacgga atcactggag
     9001 tatggtgcgc atctggtgcc agaaggtggc ttgcacagta tgccggtgca atacgccggt
     9061 aacggctggc tgctgctggg cgatgcgttg cgcagttgcg tcaataccgg aatttccgtg
     9121 cgcggcatgg atatggcgct gactggcacg caggcggcgg cacaaacgct gataagcgcc
     9181 tgccagcacc gcgagccgca aaatctgttt gcgctttatc atcacaacgt agagcgcagc
     9241 ctgctatggg atgttctaca acgttatcag catgttccgg cgcttttgca acgccctgga
     9301 tggtaccgtg cgtggcctgc gttaatgcag gatatttccc gcgatttatg ggatcagggt
     9361 gataaacctg ttccaccgct gcgccagtta ttctggcgtc atttacgtcg tcacggcctg
     9421 tggcatctgg cgggcgatgt tatcaggagt ctgcgatgtc tgtaacccgt aatctctggc
     9481 gcgttgccga tgcgccgcac attgtaccgg ctgactccgt tgagcgtcag acggtacaac
     9541 ggttgattaa cgcttgtccg gctggtcttt tttcgctcac accggaaggt gacttacgcg
     9601 ttgattatcg cggctgcctg gagtgtggca cctgccgttt gctgtgcgat gaaaaaacac
     9661 tacaacagtg gcgctatccg ccttccggat tcggcatcac ctaccgtttt ggataaataa
     9721 taaggataat ttatgcccct cttacacctg ctccgccaga atccggtgat tgctgccgtt
     9781 aaagataatg ccagcctgca actggcaatc gactctgaat gccagtttat ttccgtgttg
     9841 tacggcaata tctgcaccat tagtcaaatc gttaaaaaga ttaaaaatgc cggaaaatat
     9901 gcttttattc atgttgattt gctggaaggc gcgtcaaata aagaagtggt gattcagttt
     9961 ttgaaactgg tgactgaggc ggacggcatt atcagtacca aagcctcaat gttgaaagcg
    10021 gcaagagcgg aaggtttttt ctgtattcat cgcctgttta ttgttgattc gatttcgttt
    10081 cacaacattg ataagcaagt tgcgcaatcg aatccggatt gtattgagat cctgccaggc
    10141 tgtatgccta aagtgctggg ctgggtgacg gagaaaatcc gccaaccgct gattgccggc
    10201 gggctggtgt gcgatgaaga agatgcgcgt aatgcgatta acgcgggtgt tgtggcgctt
    10261 tccaccacga ataccggggt ctggacgtta gcgaaaaaat tgctttgacg ggatactcgt
    10321 aacgagttga atttgtgtga tttgtaggct gcacgccaca tccgacattc agcgcctgat
    10381 acgacgcttg acgcgtctta tcaggcttac aagtccccag cccagaaccg taggcaggat
    10441 catccggcag cacgttattg gcaatctgcg tgaatggttg tcaccagtgc ctccagcacc
    10501 gctttccagt catcaaccac gccaacatct gcctgtgaaa ataccgccgc gctggcgtca
    10561 tgattaatcg ccaccacaaa cttgctattg cgtacgcccg ccattaacgc tgctgcgccg
    10621 gaagcgccaa caacaatgca cacctcaggc gccagcagat gtccggaaat accaatcact
    10681 ttttcagcat caacgccacc gttcatcact cgcgcccgac tgtagcccac ttcggctccc
    10741 agcttttcta ccagcatagc gatttcctgg ttatctgcct ctccgccctg ccccaccacc
    10801 agcacccgtc tggcttcagc taatggatcg cgggtaacat ttttcaggtt ttctatgcta
    10861 atcagccagt cgggaagcgc accggggaca atgttcagtt gcagcatacc agagggcaac
    10921 atggtgttta tctctgctcc cgcctgacgc gccagggaaa gacatagcgg gcgtttttct
    10981 gtttgcagcg tcgccgttag cgcatttccc cagtgggatt tgcgcacgct tacggttgga
    11041 atatctaacg aagtcacctg gcagatgctt gcaccatgta aacgccaggc cagccggatc
    11101 gacagttcat cgccaaacgc gcccggagga aaaaggacaa cctccgcagg cgttcgttgc
    11161 cactgttcca ccagcgcatc cagcacctgt tccgcaacca tcggctgtgg ttctatttgc
    11221 caatgcgtta gtgtgctgcc gctgaaatcc tgcgccgcca gccagcccgc cattgcggca
    11281 tgttcctgat ttattgtcac aattgcgata ttcatggctg catcctttgg cgtaaatagt
    11341 cctgccatag cttttgcgct ttttctgcta ccgtttgccc atcaatcagc gccgctctgc
    11401 gtcgttgctc tggccttgcc agttgcaggc actgtatcgc tggcgcttcc gcggcaacag
    11461 tttcgcgggt aatctccgct ttccccgctg ccattcgctg gcgcattccc ggtactggca
    11521 aggccacttc accacactga cgcacggcaa tcaccgcagg caagcgaacc cggcaacagc
    11581 gcaacccatg ttcagtacgc tgttcgaggg tgataaacgg cgcgtcgaga gtaaaacgct
    11641 ccacctgggt gaaacagggc cagcccagca tttccgccag caaaaatggc gtttgtccat
    11701 tctgcccttc gctgctttgg cagccggtaa tgatcagatc cagcggattc tgacgctgcc
    11761 attcggcaat atggcgagcg acaaattccg gcgcaaagcg cagatctgcc gccgtctcca
    11821 gcaatacagc gtcctcaaac ccaagagcca tgagatagcg cagccagtgc aacgcccgtt
    11881 catcccccat gctcaacgcg gttaaagaca tcggcgtacc gttttttctc tgcgccaaca
    11941 gcagcgcggc ggcagcctgt tcatcggcac cgagtaaact tcgcagaagc gaaacatccg
    12001 gtccgatatt accctgagcc gccgcctgcc actctttttc cgccagcatt ccggcatccg
    12061 gttcggcttt aaacgctaac agaatgttca ttcgcacctc ctacatttcc cgccgccacc
    12121 agcgggagtg ctttggtttc tggtgcccat agccaggtca caacaaaacc aaccaacaac
    12181 actgccgcca ggagcaataa ggtggcttgc attccccact gcgccagcac ccacggcagc
    12241 aggccagtac ttaccgccgc ccccaaccga ctcatggcgg tggcaaaacc gacgcccagc
    12301 gagcgaatgt cagtaggaaa actttccgca ggcaaaatgc ccaccagatt actgactgcc
    12361 gaaatggtgg tgctgaagag aacaaaaagc agcagcgtta atgaactgcc ggaaggcaaa
    12421 caggccatca cgaccagcgt tgccgccagc agcaaaaaac ttcccagtaa aaatctgcga
    12481 tgcgctaaca agtgggtcaa cactaaccct aataacgcgc caacaattaa caacgcatta
    12541 agcatcaggc tggcagtcag cgcatcttcc agaccaatag tttgggcgat ggtcggcagc
    12601 caggtataaa tcacaaacca tgggattacg aggcagacaa agaagacgct gttaaacgct
    12661 gtgcgccgcc agtagcgcga agagaacaag gttttgatgt gtttatgggt cgccgttgcc
    12721 acttcatcgc ccagtaaaac atggggacca aaatagcgat gcacgatagc gtgagcttct
    12781 gcaaaacgcc cctggcgcag tagccagcgt ggcgattctg gcgttcccca gcgtaataac
    12841 gtaatcaaca atgcgggcag agctgccgat gccagtagcc agcgccaggc ctccgggctt
    12901 tcgctaataa agtgatgtcc ggcaatactt gccagcacat agcctacggt ccacaccacg
    12961 ctgaatgcgc ccagcaaaac accgcgatgg cggcgcggag aaaattcagc cagcaaagtg
    13021 tgaccgactg agtaatcgcc tcccagacca atgccgatca aaatgcgcag tccaataaga
    13081 tgctctggcg tggtggcaaa aaattgtaag aacgaagcga gcgtaatcag caaaaagctg
    13141 aaggtgaaga ttttttgccg accaatatgg tcggagatcc accccaaaac caggctaccg
    13201 ataaacaggc caagtagcgc cgagccgccg atcattcccg ccataaacgg cgtcagttgc
    13261 atggcgggtg taagctgaat aatggcgtaa ccaatgacgc cgaggacata accgtcggtc
    13321 aggtgtgcgc cgaaagtgag cgcagcaatg cggcagtgaa aacggttaag tggtaaatca
    13381 tccattcgca ccggtgaagt gttcatcttt accccttcat tgtccggtaa taagaacggc
    13441 ttctcgcctg agaagccgaa aaggcaacgc ccgtcatcgc ggaacgctgc ctgacacatt
    13501 atttttcaat cggatagata gtacccgtat tcataatgcc gttaggatcg aactgctttt
    13561 tcagcccttc cagcaacgcc cacgcgctgc cgtgttccag tttgctccag tgaacgcgat
    13621 gtttaccgat accgtggtgg tgcaccatcg aaccaccgag gcgaatggtt tcttcgcaga
    13681 tgattttgtt gagcggattg tggtacttgt cgatttcctc ttccggctta cagtcaacga
    13741 cgttgtagtc gtagacgaag tacatgttgg tgccgttctg atagctatga gaggagtgac
    13801 cgcccagcat ggtgatgtcg tcggcatgcg ggaactcggt acgaatacgg ttaataacgc
    13861 tttcgtagat ttcgtggatg cagctccagc agccggacac ttcggtggta aagcccatgt
    13921 tgccggtttt gaggatctgc acacgttcag cggccacttt atccggcccc cagttcaggt
    13981 gattaaacca ggtttcgatc agcttgctgt ccactcgctg gcattgcggg taacgtgcca
    14041 cgatttcagc aatcccttcg cctgtcgcct tcgcaatgcg agggttacct tcagccataa
    14101 agatcagcac acatttacca tcggcaaaat gggtgaagtg ttgggtgcca tcttcagcgt
    14161 catacaaacg agcgatcgac ggacgatacc cttccaccat gatttcacgc aggatgttga
    14221 agccggtttt catgtcttcc agcacatagc catagaagag attgttttcc ggcgtaaatt
    14281 tgaagatttt cactgtgact tcagtgatat agcacaatgc accttcgttg ccgatgataa
    14341 tgtgacgaat gtccgggcct gccgcgcggc gtggtacgtt tttaatacgt gtgacggtgc
    14401 catcagccaa tactgcttcc agaccaacga ccatatcttc gattgcgccg tagagtgtgg
    14461 agaactgccc gatactgcgg gttgctacca ggccgcccat ctgcgccagc ggttttgatt
    14521 gcggtgaatg ccccgtggtg taaccttttt cacgcaacgt gttttccagc acctccagcg
    14581 gaacaccaca ttgcgccgtg acctgcatat tctcaatatc aatattaatg atttgattca
    14641 tggcagagcc gtcgagcacc accgagtttt ccacaacggt ttccagtcca ccctcggtag
    14701 cggaagcacc ggtacgcggc acaccgttaa ttttgtgtgc attcataaaa ttcagtacac
    14761 gagagacttg ttctgtggaa ccgagtttta cgaccgctgc cggaatcggc aaagtataaa
    14821 tgccatgaat atccggaaat ttgcgaaaac ggtcaatact gtttttcttt aatactgttt
    14881 catcggtaat tacgcgatct gcaccaacaa tttcctttag ctggtcgaca atcgctgcgc
    14941 gagataaaga catagtaatt ccttcctgat aataaaaatg gaataatcat taataagaga
    15001 aaacagaaac caccgttatg gattagcgca ctaaataacc gccatcgacc actaataaat
    15061 gcccgttgac ataattcgat gcctgacttg cgagaaacac ggctgcgccc attaaatcct
    15121 gagtatcgcc ccaacggttt gccggaatat gatcaagaac gcgctgattg gtttctggat
    15181 tactgcgtgt cgccagcgta atatctgttg cataatagcc aggggcaata ccatttacct
    15241 gaatattata ttgacctagt tcatcacaat aagctttggt gaacccggca agagcatgtt
    15301 tagtggcaga atatgcaggt gaccattgtc cacctaagta agagaacaat gaacagatat
    15361 taataatttt accgcttttc tgcgggatca taatttttgc cgcttcatag cttaactcga
    15421 atgcggcggt caggttcaca tcaatcatcg ggtcccagtc ggcacgaccg aaatccagca
    15481 ccttattcag cttacaaata ccggcattgt taaccagaat atcaactgtt ccgaaacgct
    15541 cacagcaagc agcgataatc ttctgcggcg cgccttctgc ggtgatatcc acctgcatga
    15601 agtccatctc aacaccctgt ttttcaatca tttcctttgt tttgccgtta tctttaacga
    15661 agcttggaat aaagatattt gcaccagctt tggccagcgc catggcaaat gcctggccta
    15721 aaccgctatt cccgccggta acaattgcgg ttttaccttt cagggagaaa aaatccattg
    15781 agaacgcatt gagagattcg attgacatag ttagctccaa tttccgtaag gcaaaaaaaa
    15841 agaaaggtaa cttccccgcg agagggaagt tacctttctc attatctctg gtaactggag
    15901 ataattttta tcataagggc ataatcgcta atgtgatcgt aatcacagtg ttatattcga
    15961 attgtcaaat aattttaaaa agcagaagcg tgattactct cacacaacac cactgctttc
    16021 catgttattt cttattgcaa ttactagaga cctgagaaaa gtaatccacc tgaaagggcg
    16081 gcttactttt ctcttttttt tgtctattac aaatgggaat acgatatgca acacaactca
    16141 tatcgccgtt ggataaccct cgcgataatt agttttagcg gcggcgttag tttcgatctg
    16201 gcttatttac gttatattta tcaaattccc atggcgaaat ttatgggatt cagcaatacc
    16261 gagataggtt taataatgag tacctttggt attgcggcta ttattcttta tgcccccagc
    16321 ggcgttattg ccgataaatt ttcacaccgc aaaatgatta cttccgcgat gatcattacc
    16381 ggattactgg gtctgttaat ggcaacatat ccaccgctgt gggtaatgct ctgtattcag
    16441 gtcgcctttg cgataacgac gattttaatg ctgtggtcgg tgtcgattaa agccgcctca
    16501 ttgctgggcg atcatagtga gcaagggaaa attatgggct ggatggaagg gctgcgcggc
    16561 gtcggtgtaa tgtcgctggc ggtgtttacc atgtgggtct tttctcgctt tgcgccggat
    16621 gacagcgcca gcctgaaaac agtcattatt atctacagtg tggtttacat cttgttgggg
    16681 attctgtgct ggttttttgt tagcgataac aacaacctgc gcagtaccaa taacgaagaa
    16741 aaacagtcat tccagcttag cgatatcctg gccgttttgc gtatcagtac cacctggtat
    16801 tgcagcatgg tgatttttgg tgtcttcacc atctacgcca ttctgagtta ctccaccaac
    16861 tatctgaccg aaatgtacgg catgtcgctg gtggcggcga gctacatggg gattgtgatc
    16921 aacaaaatct tccgcgcgct gtgcggccca cttggcggca ttatcaccac ctacagtaaa
    16981 gtgaaatccc ctacccgcgt gatccaaatc ctttccgtac tcggcctgct ggcgttaact
    17041 gccctgctcg tcacgaactc taacccgcaa tcggtcgcga tggggattgg cctgatttta
    17101 ctgctgggat tcacctgtta cgcctcacgc gggctgtact gggcctgccc aggcgaagcg
    17161 agaacaccgt cttacattat gggcaccacg gtaggtattt gttcggtgat tggattcctg
    17221 ccggatgtct tcgtttaccc aattatcggc cactggcaag acaccctgcc cgcagcagaa
    17281 gcctaccgca atatgtggct gatgggcatg gcggcgcttg gcatggtgat tgtctttacc
    17341 tttttgctgt tccaaaaaat tcgtactgct gatagcgccc ccgcaatggc tagcagcaag
    17401 taagccagga aaattacagg agaaaaatca tgtcgaaaaa atacatcata gggattgatg
    17461 gcggaagtca gagcacaaaa gtggtgatgt acgatctgga aggtaatgtg gtttgcgaag
    17521 gtaaaggctt attgcagccg atgcatacgc cagatgccga tactgccgaa catcctgacg
    17581 acgatttatg gacatcgtta tgctttgccg gtcacgattt gatgagccag tttgccggaa
    17641 ataaagaaga tattgtcggt attggtctgg gctccatccg ttgctgtcgt gcgttattga
    17701 aagccgatgg cacgcccgct gcgccattga ttagctggca ggatgcacgt gtaacacgtc
    17761 cctacgatca taccaatcca gatgtggcct atgtcacctc tttttcgggt tatctgacgc
    17821 atcgcttaac cggcgagttt aaagataata tcgccaacta ttttggtcag tggccggtgg
    17881 attataagag ctggacatgg agtgaagatg ctgcggtaat ggataagttt aatatccccc
    17941 gtcatatgct gtttgatgtg caaatgcctg gcaccattct tgggcatatc acaccacaag
    18001 ctgcgctggc gacacatttc ccggctgggc tgtcggttgt ttgtaccacc agtgataaac
    18061 cggtagaagc tctgggagcc gggttactgg atgatgaaac tgcggtaatt tctcttggca
    18121 cttacatcgc attgatgatg aacggcaaag ccttgccgaa agatccggtg gcgtactggc
    18181 cgattatgtc ttctattccg caaacattgc tgtatgaagg ttacggtatt cgcaaaggca
    18241 tgtggacggt gagctggctg cgcgacatgt taggcgagtc gttaattcag gatgccaggg
    18301 cgcaggatct ttcaccggaa gatttactca acaagaaagc ctctagtgtg ccacctggct
    18361 gtaatgggct gatgacggtg ctggactggc tgaccaatcc gtgggaaccg tacaaacgcg
    18421 ggattatgat cggctttgat tccagtatgg attacgcatg gatatatcgt tcgatactgg
    18481 aaagcgtggc gctgacgctg aagaacaatt acgacaatat gtgtaatgaa atgaatcact
    18541 ttgcgaagca tgtaatcatt actggcggcg gttcgaacag cgatctgttt atgcagattt
    18601 ttgccgacgt gttcaacctt ccggcacgac gtaacgccat taacggttgt gcaagtctgg
    18661 gggcagcgat taataccgcg gtaggtctgg ggctataccc ggattacgca acggctgtcg
    18721 acaaaatgat tcgcgtgaaa gatatcttta taccgattga gagcaatgcc aaacgctacg
    18781 acgcgatgaa taaaggcatt ttcaaagacc taaccaaaca tactgatgtg atcctgaaaa
    18841 aatcgtatga agtgatgcat ggggaattgg ggaatgcgga ttcgatccag agctggtcga
    18901 atgcgtaagt gaggaaggcc gggcgggaaa ctgcccggcc tgaacatacc tgaatggtta
    18961 tccccgctga cgcggggaac ataagttcga caacttaaaa aatgataaca agtccggttt
    19021 atccccgctg atgcagggaa cataataaaa actttaacta gttcggttta tccctgtttt
    19081 tacaag
//
LOCUS       DAZFLD010000087        18747 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.087, whole genome shotgun sequence.
ACCESSION   DAZFLD010000087 DAZFLD010000000
VERSION     DAZFLD010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18747
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.087"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..541)
                     /locus_tag="ACHKBL_002560"
     CDS             complement(<1..541)
                     /locus_tag="ACHKBL_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-mannonate oxidoreductase"
                     /protein_id="HFO5819413.1"
                     /translation="MGNNLLSAKATLPVYDRNNLAPRIVHLGFGAFHRAHQGVYADIL
                     ATEHFSDWGYYEVNLIGGEQQIADLQQQDNLYTVAEMSADAWTARVVGVVKKALHVQI
                     DGLETVLAVMCEPQIAIVSLTITEKGYFHSPATGQLMLDHPMVVADVQNPHQPKTATG
                     VIVEALARRKAAGLPAFTVM"
     gene            complement(631..1998)
                     /gene="ydfJ"
                     /locus_tag="ACHKBL_002561"
     CDS             complement(631..1998)
                     /gene="ydfJ"
                     /locus_tag="ACHKBL_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000582800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter YdfJ"
                     /protein_id="HFO5819414.1"
                     /translation="MTIEKHERSTKDLVKAAVSGWLGTALEFMDFQLYSLGAALVFHE
                     IFFPESSTAMALILAMGTYGAGYVARIVGAFIFGKMGDRIGRKKVLFITITMMGICTT
                     LIGVLPTYAQIGVFAPILLVTLRIIQGLGAGAEISGAGTMLAEYAPKGKRGIISSFVA
                     MGTNCGTLSATAIWAFMFFILSKEELLAWGWRIPFLASVVVMVFAIWLRMNLKESPVF
                     EKVNDSNQPTAKPAPAGSMFQSKSFWLATGLRFGQAGNSGLIQTFLAGYLVQTLLFNK
                     AIPTDALMISSILGFMTIPFLGWLSDKIGRRIPYIIMNTSAIVLAWPMLSIIVDKSYA
                     PSTIMVALIVIHNCAVLGLFALENITMAEMFGCKNRFTRMAISKEIGGLIASGFGPIL
                     AGIFCTMTESWYPIAIMIMAYSVIGLISALKMPEVKDRDLSALEDAAEDQPRVVTAAQ
                     PSRSL"
     gene            complement(2056..3075)
                     /gene="rspB"
                     /locus_tag="ACHKBL_002562"
     CDS             complement(2056..3075)
                     /gene="rspB"
                     /locus_tag="ACHKBL_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent oxidoreductase"
                     /protein_id="HFO5819415.1"
                     /translation="MKSILIEKPNQLAIIEREIPTPSAGEVRVKVKLAGICGSDSHIY
                     RGHNPFAKYPRVIGHEFFGVIDAVGEGVESARVGERVAVDPVVSCGHCYPCSIGKPNV
                     CTTLAVLGVHADGGFSEYAVVPAKNAWKIPEAVADQYAVMIEPFTIAANVTGHGQPTE
                     NDTVLVYGAGPIGLTIVQVLKGVYNVKNVIVADRIDERLEKAKESGADWAINNSQTPL
                     GESFAEKGIKPTLIIDAACHPSILKEAVTLASPAARIVLMGFSSEPSEVIQQGITGKE
                     LSIFSSRLNANKFPVVIDWLSKGLIKPEKLITHTFDFQHVADAISLFEQDQKHCCKVL
                     LTFSE"
     gene            complement(3087..4301)
                     /gene="rspA"
                     /locus_tag="ACHKBL_002563"
     CDS             complement(3087..4301)
                     /gene="rspA"
                     /locus_tag="ACHKBL_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="starvation-sensing protein RspA"
                     /protein_id="HFO5819416.1"
                     /translation="MKIVKAEVFVTCPGRNFVTLKITTEDGITGLGDATLNGRELSVA
                     SYLQDHLCPQLIGRDAHRIEDIWQFFYKGAYWRRGPVTMSAISAVDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGHSIDEALDDYARHQELGFKAIRVQCGIPGMKTTYG
                     MSKGKGLAYEPATKGQWPEEQLWSTEKYLDFMPKLFDAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRMFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIDYIRTTLTHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCMAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPHNWTFDNGYMHPGDKPGLGIEFDEKLAAKYPYEPAYLP
                     VARLEDGTLWNW"
     gene            complement(4507..4833)
                     /gene="ynfA"
                     /locus_tag="ACHKBL_002564"
     CDS             complement(4507..4833)
                     /gene="ynfA"
                     /locus_tag="ACHKBL_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YnfA family protein"
                     /protein_id="HFO5819417.1"
                     /translation="MIKTTLLFFATALCEIIGCFLPWLWLKRNASIWLLLPAGISLAL
                     FVWLLTLHPAASGRVYAAYGGVYVCTALMWLRVVDGVKLSLYDWTGALIALCGMLIIV
                     AGWGRT"
     gene            4968..5309
                     /gene="ynfB"
                     /locus_tag="ACHKBL_002565"
     CDS             4968..5309
                     /gene="ynfB"
                     /locus_tag="ACHKBL_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1283 family protein"
                     /protein_id="HFO5819418.1"
                     /translation="MKITLSKRIGLLAFLLPCALALSTTVHAETNKLVIESGDSAQSR
                     QHAAMEKEQWNDTRNLRQKVNKRTEKEWDKADAAFDNRDKCEQSANINAYWEPNTLRC
                     LDRRTGRVITP"
     gene            5344..5904
                     /gene="speG"
                     /locus_tag="ACHKBL_002566"
     CDS             5344..5904
                     /gene="speG"
                     /locus_tag="ACHKBL_002566"
                     /EC_number="2.3.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine N1-acetyltransferase"
                     /protein_id="HFO5819419.1"
                     /translation="MPSAHSVKLRPLEREDLRYVHQLDNNASVMRYWFEEPYEAFVEL
                     SDLYDKHIHDQSERRFVVECDGEKAGLVELVEINHVHRRAEFQIIISPEYQGKGLATR
                     AAKLAMDYGFTVLNLYKLYLIVDKENEKAIHIYRKLGFTVEGELMHEFFINGQYRNAI
                     RMCIFQHQYLAEHKTPGQTLLKPTAQ"
     gene            complement(5907..6617)
                     /gene="ynfC"
                     /locus_tag="ACHKBL_002567"
     CDS             complement(5907..6617)
                     /gene="ynfC"
                     /locus_tag="ACHKBL_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YnfC family lipoprotein"
                     /protein_id="HFO5819420.1"
                     /translation="MKYKLLPCLLAIFLTGCDRTEVTLSFTPEMASFSNEFDFDPLRG
                     PVKDFTQTLMDEQGEVTKRVSGTLSEEGCFDSLELLDLENNTVVALVLDANYYRDAQT
                     LEKRVRLQGKCQLAELPSAGVSWETDDNGFVIKASSKQMQMEYRYDDQGYPLGKTTKS
                     NDKTLSVSATPSTDPIKKLDYTAVTLLNNQRVGNVKQSCEYDSHANPVDCQLIIVDEG
                     VKPAVERVYTIKNTIDYY"
     gene            6725..7030
                     /gene="ynfD"
                     /locus_tag="ACHKBL_002568"
     CDS             6725..7030
                     /gene="ynfD"
                     /locus_tag="ACHKBL_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1161 domain-containing protein"
                     /protein_id="HFO5819421.1"
                     /translation="MKLSTCCAALLLALASPAVLAAPGSCERIQSDISQRIINNGVPE
                     SSFTLSIVPNDQVDQPDSQVVGHCANDTHKILYTRTTSGNVSAPAQSTQDGAPAEPQ"
     gene            7229..9655
                     /gene="ynfE"
                     /locus_tag="ACHKBL_002569"
     CDS             7229..9655
                     /gene="ynfE"
                     /locus_tag="ACHKBL_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenate/tellurate reductase subunit YnfE"
                     /protein_id="HFO5819422.1"
                     /translation="MSKNEQMVGISRRTLVKSTAIGSLALAAGGFSLPFTLRSAAAAV
                     QQASEKVVWGACSVNCGSRCALRLHVKDNEVTWVETDNTGSDEYGNHQVRACLRGRSI
                     RRRINHPDRLNYPMKRVGKRGEGKFERISWDEALDTIASSLKKTVEQYGNEAVYIQYS
                     SGIVGGNMTRSSPSASAVKRLMNCYGGSLNQYGSYSTAQISCAMPYTYGSNDGNSTTD
                     IENSKLVVMFGNNPAETRMSGGGITYLLEKAREKSNAKMIVIDPRYTDTAAGREDEWL
                     PIRPGTDAALVAGIAWVLVNENLVDQPFLDKYCVGYDEKTLPADAPKNGHYKAYILGE
                     GDDKTAKTPQWASQITGIPVDRIIKLAREIGTAKPAYICQGWGPQRQANGELTARAIA
                     MLPILTGNVGISGGNSGARESTYTITIERLPVLDNPVKTSISCFSWTDAIDHGPQMTA
                     IRDGVRGKDKLDVPIKFIWNYAGNTLVNQHSDINKTHEILQDESKCEMIVVIENFMTS
                     SAKYADILLPDLMTVEQEDIIPNDYAGNMGYLIFLQPVTSEKFERKPIYWILSEVAKR
                     LGPDVYQKFTEGRTQEQWLQHLYAKMLAKDPALPSYDELKKMGIYKRKDPNGHFVAYK
                     AFRDDPEANPLKTPSGKIEIYSSKLAEIARTWELEKDEVISPLPVYASTFEGWDSPER
                     STFPLQLFGFHYKSRTHSTYGNIDVLKAACRQEVWINPIDAQKRGIANGDMVRVYNHR
                     GEVRLPAKVTPRILPGVSAMGQGAWHEANMSGDKIDHGGCVNTLTTLRPSPLAKGNPQ
                     HTNLVEIEKI"
     gene            9716..12139
                     /gene="ynfF"
                     /locus_tag="ACHKBL_002570"
     CDS             9716..12139
                     /gene="ynfF"
                     /locus_tag="ACHKBL_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416105.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenate/tellurate reductase subunit YnfF"
                     /protein_id="HFO5819423.1"
                     /translation="MKIHTTEALMKAEISRRSLMKTSALGSLALASSAFTLPFSQMVR
                     AAETPVEEKAVWSSCTVNCGSRCLLRLHVKDDTVYWVESDTTGDDVYGNHQVRACLRG
                     RSIRRRMNHPDRLKYPMKRVGKRGEGKFERISWDEALDTISDNLRRILKDYGNEAVHV
                     LYGTGVDGGNITNSNVPYRLMNSCGGFLSRYGSYSTAQISAAMSYMFGANDGNSPDDI
                     ANTKLVVMFGNNPAETRMSGGGVTYYVEQARERSNARMIVIDPRYNDTAAGREDEWLP
                     IRPGTDGALACAIAWVLITENMVDQPFLDKYCVGYDEKTLPANAPRNAHYKAYILGEG
                     PDGIAKTPEWAAKITSIPAEKIIQLAREIGSAKPAYICQGWGPQRHSNGEQTSRAIAM
                     LSVLTGNVGINGGNSGVREGSWDLGVEWFPMLENPVKTQISVFTWTDAIDHGTEMTAT
                     RDGVRGKEKLDVPIKFLWCYASNTLINQHGDINHTHEVLQDDSKCEMIVGIDHFMTAS
                     AKYCDILLPDLMPTEQEDLISHESAGNMGYVILAQPATSAKFERKPIYWMLSEVAKRL
                     GPDVYQTFTEGRSQHEWIKYLHAKTKERNPEMPDYEEMKTTGIFKKKCPEEHYVAFRA
                     FREDPQANPLKTPSGKIEIYSERLATIADTWELKKDEIIHPLPAYTPGFDGWDDPLRK
                     TYPLQLTGFHYKARTHSSYGNIDVLQQACPQEVWINPIDAQARGIRHGDTVRVFNNNG
                     EMLIAAKVTPRILPGVTAIGQGAWLKADMFGDRVDHGGSINILTSHRPSPLAKGNPSH
                     SNLVQIEKV"
     gene            12150..12767
                     /gene="ynfG"
                     /locus_tag="ACHKBL_002571"
     CDS             12150..12767
                     /gene="ynfG"
                     /locus_tag="ACHKBL_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMSO reductase like molybdoenzyme iron-sulfur
                     subunit YnfG"
                     /protein_id="HFO5819424.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDFKDLGPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCDDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNAEKGHMTKCDGCYSRVAEGKQPICVESCPLRALEFGPIEELRQ
                     KHGTLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPEEV"
     gene            12769..13623
                     /gene="ynfH"
                     /locus_tag="ACHKBL_002572"
     CDS             12769..13623
                     /gene="ynfH"
                     /locus_tag="ACHKBL_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenate reductase membrane anchor subunit YnfH"
                     /protein_id="HFO5819425.1"
                     /translation="MGNGWHEWPLVIFTVLGQCVVGALIVSGIGWFAANNDADRQRIV
                     RGMFFLWLLMGIGFIASVMHLGSPLRAFNSLNRIGASGLSNEIAAGSIFFAVGGLWWL
                     VAVIGKMPQALGKLWLLVSMALGVIFVWMMTCVYQIDTVPTWYNGYTTLAFFLTVLLS
                     GPILAATILRAARVTFNTTPFAIISVLALIACAGVIVLQGLSLASIHSSVQQASALVP
                     DYASLQVWRVVLLCTGLGCWLCPLIRRREPHVAGLILGLILILGGEMIGRVLFYGLHM
                     TVGMAIAG"
     gene            13666..14280
                     /gene="dmsD"
                     /locus_tag="ACHKBL_002573"
     CDS             13666..14280
                     /gene="dmsD"
                     /locus_tag="ACHKBL_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat proofreading chaperone DmsD"
                     /protein_id="HFO5819426.1"
                     /translation="MTHFSQQDNFSVAARVLGALFYYAPESAEAAPLVAVLTRDGWET
                     QWPLPEASLAPLVTAFQTQCEETHAQAWQRLFVGPWALPSPPWGSVWLDRESVLFGDS
                     TLALRQWMREKGIQFEMKQNEPEDHFGSLLLMAAWLAENGRQTECEELLAWHLFPWST
                     RFLDVFIEKAEHPFYRALGELARLTLAQWQSQLLIPVAVKPLFR"
     gene            14439..15731
                     /gene="clcB"
                     /locus_tag="ACHKBL_002574"
     CDS             14439..15731
                     /gene="clcB"
                     /locus_tag="ACHKBL_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0010447 - response to acidic pH [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated ClC-type chloride channel ClcB"
                     /protein_id="HFO5819427.1"
                     /translation="MHHLHIYPDLRTMFRRLLIATVVGILAAFAVAGFRHAMLLLEWL
                     FLNNDSGSLVNAATNLSPWRRLLTPALGGLAAGLLLMGWQKFTQQRPHAPTDYMEALQ
                     TDGQFDYAASLVKSLASLLVVTSGSAIGREGAMILLAALAASCFAQRFTPRQEWKLWI
                     ACGAAAGMAAAYRAPLAGSLFIAEVLFGTMMLASLGPVIISAVVALLVSNLINHSDAL
                     LYSVQLSVTVQARDYALIISTGVLAGLCGPLLLTLMNACHRGFVSLKLAPPWQLALGG
                     LIVGLLSLFTPAVWGNGYSTVQSFLTAPPLLMIIAGIFLCKLCAVLASSGSGAPGGVF
                     TPTLFIGLAIGMLYGRSLGLWFPDGEEITLLLGLTGMATLLAATTHAPIMSTLMICEM
                     TGEYQLLPGLLIACVIASVISRTLHRDSIYRQHTAKHS"
     gene            complement(15684..16379)
                     /gene="bioD"
                     /locus_tag="ACHKBL_002575"
     CDS             complement(15684..16379)
                     /gene="bioD"
                     /locus_tag="ACHKBL_002575"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HFO5819428.1"
                     /translation="MLKRFFITGTDTSVGKTVVSRALLQALASQGKTVAGYKPVAKGS
                     KETPEGLRNKDALVLQSVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANL
                     TDKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
                     NDGLPLIGWVANRINPGLAHYAEIIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLA
                     MLRSVLAVDRVTV"
     gene            complement(16504..17724)
                     /gene="mlc"
                     /locus_tag="ACHKBL_002576"
                     /gene_synonym="dgsA"
     CDS             complement(16504..17724)
                     /gene="mlc"
                     /locus_tag="ACHKBL_002576"
                     /gene_synonym="dgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar metabolism global transcriptional
                     regulator Mlc"
                     /protein_id="HFO5819429.1"
                     /translation="MVAENQPGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRLAQLAP
                     ASITKIVREMLEAHLVQELEIKEAGNRGRPAVGLVVETEAWHYLSLRISRGEIFLALR
                     DLSSKLVVEESQELALKDDSPLLDRIISHIDQFFIRHQKKLERLTSIAITLPGIIDTE
                     NGIVHRMPFYEDVKEMPLGEALEQHTGVPVYIQHDISAWTMAEALFGASRGARDVIQV
                     VIDHNVGAGVITDGHLLHAGSSSLVEIGHTQVDPYGKRCYCGNHGCLETIASVDSILE
                     LAQLRLNQSMSSMLHGQPLTVDSLCQAALRGDLLAKDIITGVGAHVGRILAIMVNLFN
                     PQKILIGSPLSKAADILFPVISDSIRQQALPAYSQHISVESTQFSNQGTMAGAALVKD
                     AMYNGSLLIRLLQG"
     gene            complement(17859..18746)
                     /gene="ynfL"
                     /locus_tag="ACHKBL_002577"
     CDS             complement(17859..18746)
                     /gene="ynfL"
                     /locus_tag="ACHKBL_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO5819430.1"
                     /translation="MELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQIG
                     ARLLARTNRSVLLTAAGKQFLADSRQILSMMDDAAARAERLHQGEAGELRIGFTSSAP
                     FIRAVSDTLSLFRRDYPDVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPETLEHAV
                     IVHEPLMAMIPHDHPLANNPSVTLAELAKEPFVFFDPHVGTGLYDDILGLMRRYNLTP
                     VITQEVGEAMTIIGLVSAGLGVSILPASFKRVQLNEMRWVPIAEEDAVSEMWLVWPKH
                     HEQSPAARNFRIHLLNALR"
BASE COUNT         4819 a         4813 c         4508 g         4607 t
ORIGIN      
        1 acatgacggt aaatgcggga agtcctgccg ctttacggcg agccagcgcc tcgacaatca
       61 cccctgttgc agttttcggc tggtggggat tttgtacgtc ggcaacgacc atcgggtgat
      121 cgagcattaa ctgtccggtc gccggagagt ggaaataccc tttttcggtg attgtcagag
      181 agacaatcgc gatttgcggt tcacacatca cagccaacac ggtttctaag ccatcaatct
      241 gtacgtgcaa ggctttttta acgacgccaa cgacgcgagc cgtccacgca tcggccgaca
      301 tttccgcaac ggtataaaga ttatcttgct gttgtaaatc ggcaatttgc tgttcgccgc
      361 cgattaaatt gacctcataa tatccccagt cactgaaatg ttccgtagca agaatatcgg
      421 catacacacc ctgatgcgca cggtgaaatg caccaaagcc taaatgaaca attcttgggg
      481 ccaggttatt acgatcataa acagggagcg tcgcttttgc tgataacaaa ttatttccca
      541 taacaattcc ttaaatttaa atatggcaag ctgcatgttt tgttatatga ataaaaaatc
      601 ccctctccgg taagagaagg gatggaggat ttacagactt cttgaaggtt gcgcagctgt
      661 tacaacacgc ggttgatctt ccgcggcatc ttccagtgca cttaaatcac ggtctttcac
      721 ttctggcatt ttcagcgcag agattaaacc aatcactgaa tatgccatga tcataatggc
      781 gatcggatac caggattccg tcatggtgca gaaaataccc gccaggatag gaccaaaacc
      841 ggaagcgata agaccaccaa tttctttaga aatggccatc cgggtaaagc ggtttttaca
      901 gccgaacatt tctgccatgg taatgttttc cagagcaaat aatcccagca ccgcacagtt
      961 atgaatcaca atcagtgcca ccataatggt gctcggggca tagcttttat ctacgatgat
     1021 agaaagcatt ggccatgcca gcacaatcgc ggaggtattc ataataatat acgggatccg
     1081 gcgaccaatt ttatcggata accaaccaag gaacggaatg gtcataaagc cgagaatcga
     1141 actgatcatc aatgcatctg ttggaattgc tttgttaaac aataacgtct gtactaaata
     1201 gcctgcaagg aaagtctgaa ttaaaccgga gttaccagcc tgaccaaaac gcagccctgt
     1261 tgccagccag aaggatttgc tctggaacat gctaccagca ggtgcaggtt ttgctgtcgg
     1321 ttggttgctg tcgttaacct tctcaaagac cgggctttct ttcagattca tacgcaacca
     1381 gatagcaaag accatcacga caacgctcgc caggaacggt atacgccatc cccacgccaa
     1441 cagttcctct ttactgagaa tgaagaacat aaaggcccag attgccgttg cgcttaaggt
     1501 tccgcagtta gttcccatag ccacaaatga ggagataatt ccgcgcttac cttttggcgc
     1561 atattccgcc agcatcgtac cggcaccgga aatttccgca cctgcaccca atccctgaat
     1621 aatacgcagc gtcaccagca agatgggtgc aaaaacacca atctgtgcat aggtcggtaa
     1681 cacaccaatt aaggtggtac agatccccat catggtgatg gtaataaaga gtactttttt
     1741 acgccctatt ctgtcgccca ttttgccgaa aataaatgct ccgacaatac gcgccacata
     1801 acctgcaccg taggttccca ttgccagaat taacgccatt gccgttgatg attcaggaaa
     1861 aaatatttca tgaaacacta acgctgcacc gagcgaatat aactggaaat ccataaattc
     1921 aagcgcagtg cccagccatc ccgatactgc tgctttcacc aaatccttag tgcttctttc
     1981 gtgtttttct attgtcataa tggttatctc taaaaaagag gtaagatgcg tactacttac
     2041 tcgccgttat tggtattatt cagaaaaagt aagtaagact ttgcagcaat gtttttgatc
     2101 ctgttcaaat aaactaatgg catcagcaac atgctggaaa tcaaacgtat gggtaattaa
     2161 tttttctggt ttaattaacc ctttacttaa ccagtcgata acgaccggaa atttatttgc
     2221 atttaagcgt gaagagaaaa tagagagttc ttttccggta attccttgct gaatcacttc
     2281 agacggttca ctggagaagc ccatcaatac aatacgtgcc gctggagaag ccagcgttac
     2341 agcctctttc aggatagaag gatgacaagc cgcatcgata attaatgtcg gcttgatgcc
     2401 tttttcagcg aaactctcgc caagcggtgt ctggctgtta ttaatcgccc agtctgcccc
     2461 gctctctttc gctttttcca gtcgttcatc aatgcgatcg gcaacaatca catttttaac
     2521 gttatagacg ccttttaata cctgaacgat cgtcaggccg attggaccgg caccgtaaac
     2581 cagaacggta tcattttcag tcggttgacc atgaccggta acgttagccg caatggtaaa
     2641 aggttcgatc atcaccgcat attgatcggc cactgcttca ggaattttcc acgcattttt
     2701 tgccggaacc acggcatatt cactgaaacc accgtcagcg tgcacaccta atacagccag
     2761 tgtcgtacaa acgttcggct tacctataga gcacggatag caatgcccac agctgaccac
     2821 cggatcgaca gcaacgcgtt caccgactct ggcgctttcc acgccttcac ccactgcatc
     2881 aatgacgcca aagaattcat gaccaatgac gcgcggatat ttcgcaaaag gattatgccc
     2941 acgataaata tggctatctg aaccacaaat tccggcaagt ttcactttta ctcgtacttc
     3001 acccgctgac ggggtgggta tttcgcgttc gataattgcc agttgattcg gtttttcaat
     3061 taaaatgctt ttcattatct tactccttac cagttccaca gcgtgccatc ttccagacgt
     3121 gcgactggca gataagcagg ttcataggga tatttcgccg ccagcttttc atcgaattcg
     3181 atgccaagac ccggtttgtc tcccggatgc atatagccgt tatcgaaagt ccagttgtgc
     3241 gggaagactt caagcatttg ttcggaataa cccatgtact cctggacacc gaaattgggg
     3301 acccacagat caaagtgcag cgccgcagcc atgcagactg gcgacaaatc ggaaggaccg
     3361 tgtgagccag tacgtacctg atacagcgaa gcaaaatcgg caatccggcg cataccggta
     3421 atcccccccg cgtgagtcag cgtggtgcgg atatagtcga taagttgttc ttcaatcagc
     3481 tgtttgcagt cccagatgct gttgaagact tcacccactg caattggcgt gacggtgtgt
     3541 tggcgaatca ggcggaaaca ctcctggttt tccgcaggcg tcgggtcttc catccagaac
     3601 atgcgataat cttcaatgct tttaccaaag cgcgccgctt caataggcgt taagcgatgg
     3661 tgcatgtcat gcagcaaatg ttcattaaaa ccaaacttgt tacgtaccgc gtcaaacaat
     3721 ttcggcatga aatcgaggta tttctccgtc gaccacagct gctcttccgg ccactgtcct
     3781 ttggttgcgg gttcataagc cagaccttta cctttcgaca tgccataggt ggttttcata
     3841 ccagggattc cgcactgcac gcggatggct ttgaatccca gctcctgatg acgggcataa
     3901 tcatccagag cttcatcaat actgtgaccg gtggtatggc aataaaccat caccccttca
     3961 cgagacgcgc cgccgagtaa ctggtaaagc ggcatgttgg cagctttggc tttaatatcc
     4021 cacagcgcca tatcaaccgc tgaaatggcc gacatcgtaa ccggaccgcg acgccagtaa
     4081 gcacctttat agaaaaactg ccagatatct tcgatacggt gtgcatcgcg accaataagc
     4141 tgcggacaaa ggtgatcctg caaataagag gccacggaaa gctcacgtcc attgagggtg
     4201 gcatccccaa ggcccgtaat accgtcctca gtggtgattt ttaatgtgac gaaattacgc
     4261 cccggacagg taacaaaaac ttcagccttt acgatcttca tgttcgattc cttgcatcgc
     4321 ttgtcgtgat gcatgaaatc tacgcaactg agctactacc atacaagtat gaagatcaaa
     4381 aaaagccgga gtgatcacaa aaaaaggcgt atatttgcgc cgtgaatggt tgacaaaaga
     4441 tgaaatagaa taccttttgt cagctgacac ttcctcgtat cttattgata aaagggattt
     4501 atgttcctac gtgcgccccc agcccgcaac aatgatcaac atgccgcaaa gcgcaatcaa
     4561 cgcccccgtc cagtcataaa gactcagttt cacgccatcc acaacgcgca gccacatcaa
     4621 cgccgtgcag acataaacgc caccataagc ggcgtaaaca cgcccactcg ccgcaggatg
     4681 caacgttaac aaccagacaa acagcgccag tgaaatcccc gccggaagca acagccagat
     4741 actggcgttt cgttttaacc acaaccaggg cagaaagcat ccaataattt cacacagcgc
     4801 agtagcaaaa aatagtaacg ttgttttaat catctttgtc acttattgac atcatgtata
     4861 gttataggac gacataatat catcaatata aacatcctcc tggtacgttt tgcgttcgca
     4921 gtggatggtg tagaatcacc tttaatcatt catacaggga atgaattatg aaaattactc
     4981 tcagcaaacg aattggcctg ctcgcttttc tgctgccttg cgcactggca ttgagcacaa
     5041 ctgttcatgc cgaaactaac aaactggtga ttgagtctgg cgacagtgca caaagccgcc
     5101 agcacgccgc tatggaaaaa gagcaatgga atgacacgcg caatctgcgc cagaaagtga
     5161 ataaacgcac tgaaaaagag tgggataaag ccgacgccgc ttttgataac cgcgataaat
     5221 gtgagcaaag cgccaacatc aatgcctact gggagccaaa tactttgcgc tgcctggacc
     5281 gtcgaactgg ccgcgttatt accccctaac ctgttattga tttaaggaat gtaaggacac
     5341 gttatgccaa gcgcccacag tgttaagcta cgcccgctgg agcgtgaaga tttacgctat
     5401 gtacatcaac tcgacaataa cgccagtgtg atgcgttact ggtttgagga accctacgaa
     5461 gcctttgttg aactctctga tctgtacgat aagcatattc acgatcagag cgaacggcgc
     5521 tttgtggtgg aatgtgacgg cgaaaaagcc ggtctggtgg agctggtgga aattaaccat
     5581 gttcatcgcc gcgcagaatt tcagataatt atctccccgg aatatcaggg gaaaggtctg
     5641 gcaacgcgtg ccgcaaaatt agcaatggac tatggcttca ccgttctcaa tctctataag
     5701 ctgtatctga tcgtcgataa agagaatgaa aaagcgattc acatttaccg caagcttggc
     5761 tttacggttg aaggtgaatt gatgcatgag ttctttatta atggtcaata tcgtaatgcc
     5821 attcgcatgt gcatattcca gcatcagtat ctggcagagc acaaaacacc gggtcagact
     5881 ctcctgaagc cgaccgcaca atagcattaa taataatcga tagtattttt gatggtgtaa
     5941 acccgttcga cggcgggttt tactccttca tcaacaatga ttagctgaca gtccaccgga
     6001 ttggcgtgac tgtcatattc acagctctgt tttacattac caaccctttg attattcagt
     6061 aaagtaaccg ctgtgtaatc taattttttg atcggatccg ttgatggcgt agcgctgaca
     6121 gataatgttt tgtcgttact tttcgtggtt ttacccagcg gatacccctg atcatcatag
     6181 cgatattcca tctgcatctg tttgctgctg gctttaatca caaagccatt atcatcggtt
     6241 tcccagctca ccccggcaga aggtaattct gctagctggc attttccctg caaacgaact
     6301 ctcttctcca gtgtctgggc atcacggtaa taattggcgt ccagtaccag ggcaaccacg
     6361 gtattatttt ccagatccag taattcgagt gaatcaaaac agccttcttc cgacaaagtc
     6421 cccgaaacac gtttcgtcac ttcaccttgc tcatccatta atgtctgagt gaaatctttt
     6481 accggaccac gcagcggatc aaaatcgaat tcattagaga aactggccat ctcaggggta
     6541 aatgaaagtg ttacctctgt gcggtcacat cctgtgagga atatcgcgag taagcatggc
     6601 agtaatttgt atttcacaac agtcaccaga gagtagagat gattctcaat catagtagca
     6661 aatacagtac tttacacgtt aaatgctatg cttaaagaag ttatcttcgc gtaaggagct
     6721 tatgatgaaa ctctcaacct gctgtgccgc gcttctgctc gccctcgcct cacccgcggt
     6781 actcgccgcg ccgggatcct gtgagcgcat acagagcgat atatcacagc gcattatcaa
     6841 taatggcgta cccgaaagca gcttcacgtt aagtattgta cccaatgacc aggttgatca
     6901 gcctgattcc caggtcgtcg ggcattgcgc taatgatacg cataaaattc tctacacccg
     6961 caccaccagc ggtaacgtct ctgctcccgc gcagtctacc caggatggcg cgcctgccga
     7021 accgcagtaa tacattatcg actgcacgcc ggatacgaca catccggcga tttgaacata
     7081 caacataatc ccaccttatt actcataccc ttctattgat atggattaat aattcttaac
     7141 ccaaaatagg tagactccct ctattgttag cgcgctaaat attcaatata taaactttta
     7201 tataacgata aagaacaggg agtgagttat gtccaaaaat gaacaaatgg tgggcatcag
     7261 ccgcagaaca ctcgttaaat ctaccgcgat aggttctctg gcgctggctg caggcggttt
     7321 ttctttaccg ttcaccctgc gcagtgcagc ggcagcggta cagcaggcca gcgagaaagt
     7381 ggtctggggt gcctgttccg tcaactgtgg tagccgctgt gcacttcgtc tgcatgttaa
     7441 agataatgaa gtgacctggg tggaaactga caacaccggc agcgatgagt atggcaatca
     7501 tcaagtacgc gcctgtttgc gcggtcgctc catccgtcgg cgtattaatc atcccgaccg
     7561 cttgaattac ccaatgaaac gcgtgggcaa acgcggcgaa ggcaaattcg aacggattag
     7621 ctgggatgaa gccctggata ctatcgccag tagcctgaaa aaaaccgtcg aacaatatgg
     7681 caatgaggct gtatatattc agtactcttc agggatcgtt ggcggcaata tgacccgctc
     7741 ttcgccatcc gcctcggcgg tcaaacgcct gatgaactgc tacggcggtt cactcaacca
     7801 gtatggctcc tacagcaccg cgcaaatctc ctgtgccatg ccctacacct acggcagtaa
     7861 tgatggcaac agcaccacgg atattgaaaa cagcaagctg gtcgtgatgt ttggtaacaa
     7921 cccggctgaa acccgcatga gcggcggtgg catcacttat cttcttgaaa aagcgcgcga
     7981 gaaatcgaac gcaaaaatga ttgttatcga tccgcgatat accgatacgg ctgctggtcg
     8041 tgaagatgaa tggcttccta ttcgcccggg caccgatgcc gcgctggttg cgggtattgc
     8101 ctgggtattg gttaacgaaa atctcgttga tcaacctttt ctcgataaat actgcgtcgg
     8161 ctatgacgaa aaaaccttac ccgcagatgc acccaaaaat ggtcactata aagcctatat
     8221 tcttggtgaa ggtgatgata aaacagcgaa aacgccgcag tgggcttcgc aaattacagg
     8281 tatcccggta gaccgtatca tcaaactggc gcgtgaaatt ggcacagcaa aacccgctta
     8341 tatctgccag ggctgggggc cgcaacgcca ggcaaacggc gagctgacgg cacgcgctat
     8401 tgctatgttg cctattttga cgggcaatgt cggcatcagc ggcggaaata gtggtgcgcg
     8461 tgaatcgacc tacaccatta ccatcgaacg cctgccggtg ctggataatc cggtcaaaac
     8521 gtcaatctcc tgcttcagtt ggacagatgc tatcgatcat ggcccgcaaa tgacggcaat
     8581 ccgcgacggc gtccggggca aagataaact ggatgtgccc attaagttca tctggaacta
     8641 cgcgggaaat accctggtta atcagcattc tgacatcaac aaaacgcatg aaattctgca
     8701 ggacgaatcg aaatgcgaaa tgattgtggt catcgaaaac tttatgacct catcggcaaa
     8761 atatgccgac attctgctgc cagacctgat gaccgttgag caggaagata ttattcctaa
     8821 cgactacgcc ggtaacatgg gatatctcat tttcctccag cctgtcacca gcgaaaaatt
     8881 cgaacgcaaa ccaatttact ggatcctgag tgaagtcgcg aaacgtcttg gacctgacgt
     8941 ctatcaaaag ttcactgaag gtcgcacgca ggaacaatgg ttacaacatc tgtacgccaa
     9001 aatgcttgcc aaagatccgg cgttaccgtc ttacgacgaa ttaaaaaaaa tgggtatcta
     9061 taagcgtaaa gatcccaatg gtcattttgt cgcctacaaa gcatttcgtg acgaccccga
     9121 ggcaaatcca cttaaaacgc cttccggtaa gattgaaatt tattccagca agctggcgga
     9181 aattgcccgt acctgggaac tggaaaaaga tgaagtgata agcccactgc cggtttatgc
     9241 ctcaaccttt gaaggctggg actcccctga acgtagtacc tttccactgc aactgtttgg
     9301 tttccattac aaatcccgta ctcactcgac ctacggcaac attgatgtcc tgaaggctgc
     9361 ctgccgtcag gaggtgtgga tcaaccctat agacgcacag aaacgtggga ttgccaacgg
     9421 cgatatggtg cgggtgtata accatcgtgg cgaagttcgg ctaccagcca aagtgacacc
     9481 acgcattctc cctggggtta gcgctatggg ccagggagcc tggcacgagg ccaatatgtc
     9541 tggcgataaa atcgaccatg gcggctgtgt gaatacgcta accactctgc gcccttcacc
     9601 actggcgaag ggaaacccgc agcacactaa tctggtcgag atcgaaaaaa tataacccac
     9661 tacaaccata aattctggca tgacattttg tttgaaaagc aataagtgag taatgatgaa
     9721 aatccatacc acagaggcgc tcatgaaggc tgaaatcagc cgtagaagtc tgatgaaaac
     9781 ctccgcactt ggcagtctcg cgctggcaag cagtgctttc actctgccat tttcccaaat
     9841 ggtccgagcg gcagagacac cggtagaaga gaaagcggtc tggagttcct gcaccgttaa
     9901 ctgcgggagc cgctgtctgt tacgtttgca tgtgaaagat gacaccgtgt actgggtgga
     9961 gtctgatacg acaggagacg acgtttacgg taatcatcag gttcgggcat gtttacgcgg
    10021 gcgctctatc cgccgacgga tgaatcatcc tgataggttg aaatatccca tgaagcgcgt
    10081 cggcaagcgc ggtgaaggta aatttgaacg gataagttgg gatgaagccc tggataccat
    10141 cagtgataat cttcggcgga tccttaaaga ttacggcaat gaggctgtac atgtcctgta
    10201 cggaacaggc gtagatggcg gaaacatcac caactcgaac gtcccgtacc gtctgatgaa
    10261 ctcttgcggt ggttttctca gtcgctatgg cagctacagt accgctcaaa tcagtgccgc
    10321 aatgagttat atgttcggtg ccaatgatgg caacagcccg gatgatatcg ccaatacgaa
    10381 actggtcgtt atgttcggaa ataacccggc agaaacccgg atgagcggcg gtggtgtcac
    10441 ttactacgtc gagcaagccc gagaacgttc aaacgcacgc atgatcgtca tcgatccacg
    10501 ttataacgac acagctgccg ggcgtgaaga tgaatggctg ccaattcgcc caggcaccga
    10561 tggcgcactg gcctgtgcga ttgcctgggt actgattact gaaaacatgg tcgatcagcc
    10621 atttctcgac aaatattgtg ttggttacga tgaaaaaacg ctgcccgcca acgcaccacg
    10681 taacgcgcat tataaagcct atattctggg cgaagggcct gacggcatag ctaaaacgcc
    10741 ggaatgggca gcaaaaatca ccagcatccc ggcagaaaaa attatccagt tggcacgaga
    10801 gatcggttca gcaaaacctg cttatatttg tcagggttgg gggccacaac gacactccaa
    10861 cggcgaacaa acgtcccgcg ctattgccat gctttccgtt ctcaccggta acgtcggcat
    10921 aaacggcggc aactcaggtg tacgcgaagg tagctgggat ctgggggtag aatggttccc
    10981 gatgcttgag aatcctgtta aaacgcagat ttccgtcttt acatggacag atgccatcga
    11041 ccacggtacg gaaatgaccg cgacccgtga tggtgttcgt ggaaaagaaa aactggatgt
    11101 ccccatcaag tttttatggt gctacgccag taacacattg atcaatcaac atggcgacat
    11161 caatcacacc catgaggtgc ttcaggatga cagcaagtgc gagatgattg ttggcattga
    11221 ccacttcatg actgcctcgg ctaagtattg cgatatcctg ttgcccgacc tgatgccaac
    11281 agagcaagaa gaccttatct ctcacgaatc tgccgggaat atgggctatg tgatcctcgc
    11341 ccaacccgca acctcagcaa aatttgaacg caaacccatc tactggatgc tgagcgaagt
    11401 tgccaaacgc ttaggaccag acgtttatca aacctttact gaaggccgca gtcagcacga
    11461 atggatcaaa tatctccatg cgaaaacgaa ggaacgtaac cctgagatgc ccgactacga
    11521 ggagatgaaa acgaccggga tctttaagaa aaaatgcccg gaagaacact acgtcgcttt
    11581 ccgcgcattc cgtgaagatc cacaggcaaa cccgttgaaa acaccttcgg ggaaaatcga
    11641 aatttattct gaacgactgg cgacgattgc ggatacctgg gaattgaaaa aagatgaaat
    11701 tattcatccc cttcctgcgt ataccccagg ttttgatggc tgggacgatc ccctgcggaa
    11761 aacctatcca ctgcagctaa cgggcttcca ctacaaagcg cgtacccact ccagctacgg
    11821 caatattgat gtgttacagc aggcctgccc acaagaggtg tggatcaacc ctattgatgc
    11881 tcaggcacgc ggtatccgtc atggcgatac cgtgcgggta tttaacaata atggagaaat
    11941 gctgattgcc gcaaaagtga ctccgcgtat tctgcctggc gttaccgcca tcgggcaagg
    12001 tgcgtggctt aaagcggata tgtttggtga ccgggtcgat cacggcggca gtatcaatat
    12061 tctgacctct caccgccctt caccgctggc aaagggaaac ccgtcgcaca gcaatcttgt
    12121 ccagatcgaa aaggtttaag gagtagccca tgaccacaca atatggattt tttatcgatt
    12181 ccagccgctg tactggctgt aaaacctgcg aactggcgtg caaagatttt aaagatcttg
    12241 gcccggaagt cagtttccgc cgtatttacg aatatgctgg cggcgactgg caggaagata
    12301 atggcgtctg gcaccaaaac gtgttcgctt attatctctc catctcctgt aaccattgcg
    12361 atgaccccgc atgtacaaaa gtctgcccca gcggcgcaat gcataagcgt gaagatggct
    12421 ttgtggtggt tgatgaggat gtttgtatcg gctgccgcta ctgccacatg gcttgtccgt
    12481 acggcgcgcc acagtacaat gctgaaaaag ggcacatgac gaagtgcgat ggttgttatt
    12541 cgcgcgtcgc cgaggggaaa caacccatat gtgtcgaatc ctgcccgctg cgtgcacttg
    12601 agttcggtcc aattgaagag ctgcgccaga agcacggcac tctggctgcc gtcgcgccac
    12661 taccgcgcgc gcatttcaca aaacccaata tcgttatcaa acctaacgcc aacagccgcc
    12721 cgaccggcga taccactggt tatctggcta atccggagga agtgtaaaat gggaaatgga
    12781 tggcatgaat ggccactggt tatctttacc gtattgggtc aatgcgtagt aggcgcgctg
    12841 attgttagcg gaattggctg gttcgccgcc aataatgacg cagaccgtca gcgtattgtc
    12901 cgtggcatgt ttttcctctg gctgttgatg ggcatcgggt ttattgcctc tgtcatgcac
    12961 ctgggatcgc cactgcgagc gtttaactcg ctgaatcgaa tcggagcttc tggtctgagt
    13021 aacgagattg ctgccggttc catatttttt gccgttggcg gattatggtg gctggtggca
    13081 gtcatcggta aaatgccgca agcgttgggg aaactctggt tacttgtcag catggcgctc
    13141 ggcgtcattt tcgtctggat gatgacctgc gtgtatcaaa tcgacacagt gccaacctgg
    13201 tataacggtt acactacgct ggctttcttc ctgacagtat tgttgagcgg tccaatactt
    13261 gctgccacaa ttctgcgggc agcccgcgtt acatttaata ccacaccatt tgccatcatt
    13321 agcgttctgg cgctgatcgc ttgtgccggg gtgattgtgc tgcaaggttt gtctctggcg
    13381 tctattcact catccgtaca acaagccagc gcactggtac cagattacgc ctccttacag
    13441 gtctggcgtg tggtattgtt atgcacaggt ctaggatgct ggctttgtcc gctcattcgt
    13501 cgccgtgaac ctcacgttgc cggacttatt ctggggctaa tcctcatact tggcggtgag
    13561 atgatcgggc gtgtgctctt ttatggcctg cacatgacgg taggaatggc tattgcaggt
    13621 taacaaattg gtgcggggca acccgcacat ttcaggatgt taggaatgac ccatttttca
    13681 cagcaagata atttttctgt cgcggcgcgc gtgttgggcg cgctgtttta ttacgctcca
    13741 gagagtgcgg aagccgcacc tctggttgcg gtactcaccc gtgacggctg ggaaactcag
    13801 tggcctctac cagaagcgtc attagcgccg ctggtgacgg cttttcagac acagtgtgaa
    13861 gagactcacg cccaggcctg gcagcgtttg tttgtcggcc cgtgggcact gccgtctccg
    13921 ccgtggggat cggtctggct ggatcgcgaa tctgtgctgt ttggcgattc aacattggca
    13981 cttcgtcagt ggatgcgcga gaaaggcatt cagtttgaaa tgaagcaaaa cgaaccggaa
    14041 gatcattttg gttcactgct gttgatggct gcgtggttgg cggagaatgg tcgccagaca
    14101 gaatgcgaag aattgctggc atggcacctt tttccgtggt caacacgatt tcttgacgtt
    14161 tttatcgaaa aagcagaaca ccctttctac cgtgcactgg gggaactggc tcgcttaacg
    14221 ctggcgcaat ggcaatcaca actgttgatt cctgtcgcgg ttaaaccact ctttcgatag
    14281 gatcaccgta atattgccgg atggtggcga ttacgcgcaa ttcggcaata ataggttaca
    14341 gtgtcacgtt tttttatctc ttaaagcacg cactgctttt gcggctggcc tcttttgccg
    14401 caaaatagtc gcccgtgttt cattgcccat ttctgctcat gcatcatcta cacatctatc
    14461 cggatctgcg cactatgttc cgccgtctgc ttatcgcaac agtcgtcggt attcttgcgg
    14521 cctttgccgt tgccgggttt cgtcatgcaa tgctgctact ggagtggttg ttcctcaata
    14581 atgactcggg cagtctggtc aatgcagcga caaatctttc cccatggcga cggctgctaa
    14641 ctccggcgct cggcggactg gcggcgggtt tgttgctgat gggctggcag aaatttaccc
    14701 agcaacgccc tcatgcgccg accgattaca tggaagcgtt gcaaaccgat gggcagtttg
    14761 attacgcagc aagcctggtt aaatcgcttg cctctctgct ggtagtaacc agcggcagtg
    14821 caattggtcg cgaaggtgcg atgattcttt tagctgccct tgccgcctcc tgttttgccc
    14881 aacgttttac gccacgccag gagtggaaat tatggatcgc ctgtggagct gcggcgggaa
    14941 tggctgcggc ctatcgcgcc ccgcttgctg gcagtttatt tatagccgaa gtgctgtttg
    15001 gcactatgat gttggcctct ctcggcccgg tgattatttc cgccgtcgtg gcgttgctgg
    15061 ttagcaatct gattaatcat agcgacgcgt tactctacag cgtacaactc tcagtgacgg
    15121 ttcaggctcg tgactatgcg ctgattatca gtacaggtgt gctggcaggt ctgtgcggac
    15181 cactgttgtt aacgttaatg aacgcctgtc atcgtggatt tgtaagtctc aaacttgcgc
    15241 cgccctggca actggcactg ggcgggttga tcgtgggtct gctttccctg ttcacacctg
    15301 cagtgtgggg caacggctat agcaccgtac aatccttttt aaccgccccg ccactgttaa
    15361 tgatcattgc cgggatcttc ctttgtaaac tgtgtgccgt gctggcgagt agcggttccg
    15421 gcgcacccgg tggtgtcttt acaccgacgc tatttatcgg tcttgccatt ggcatgttgt
    15481 atggtcgtag cctgggatta tggttccctg atggcgaaga aattacgctt ttactcggat
    15541 tgaccgggat ggcgacactg ttggcggcaa ccacgcacgc gccgattatg tcgacgttga
    15601 tgatatgtga aatgaccggg gagtatcagc tactccccgg tttattgatt gcctgcgtaa
    15661 ttgcgtcggt aatttcgcgg acgttacacc gtgactctat ctaccgccag cacactgcga
    15721 agcatagcta agcggatgta ttgccccagt tcacgctgtt cggcgcgcgg cagataaggc
    15781 agttcaccaa tgagcggtgc cggaagtttt ttaccgagca catcaatgat ttccgcataa
    15841 tgcgccagtc ctggattgat tcggttagcc acccagccaa tgagcggcag cccgtcgttg
    15901 gcaatcgcct gagctgttag tagtgcatgg ttaatgcaac cttcctgaat accgacaacc
    15961 atcaataccg gcagttgttc ctgcaccacc cattcagaga gcggacgcaa atcattcatc
    16021 agactgcgcc agccgccagt cccttctacc acgacatgat cgactttatc ggtcaggttt
    16081 gccaggccgt ttgaaatgag ggtgtaattg attgggcaac tgtgcgccac gctactttct
    16141 tcttcgctta acgcgatagg attaactgct tcataaggca gttcgatggt tgaaacactc
    16201 tgcaacacca gggcatcttt attacgcagc ccttcgggtg tctctttgct ccccttcgct
    16261 acgggtttat atcccgcaac cgtttttccc tgggaggcta acgcttgtag caatgcgcgg
    16321 gaaaccaccg ttttccctac agaagtgtct gtaccggtaa taaagaaacg cttcagcatc
    16381 actaactcca ccgttatgct tcacaaatat aaaccaggaa aataattaac cttgaaagtc
    16441 taagttatgc tttcctggcc caaattgaga tagcgcaaat tttggtagaa cagttaaaaa
    16501 atgttaaccc tgcaacagac gaatcaacaa agaaccgtta tacatcgcgt cttttaccag
    16561 cgcagcccct gccatcgtac cctggttaga aaactgagta ctctcaacgc taatgtgctg
    16621 actatacgca ggaagggcct gctgacggat gctgtccgag atgaccggga agaggatatc
    16681 tgccgcttta cttaacggtg agccaatcag tattttttgt gggttaaata aattcaccat
    16741 gatggcaaga atgcgcccta catgcgcgcc caccccggta atgatgtctt ttgccagtag
    16801 atcgccgcgc aatgccgcct gacacaatga atccacggtt aacggctgtc catgtaacat
    16861 cgagctcatg gattgattga gacgcagctg tgccagctca agaatactgt ccacactggc
    16921 gatggtttcg aggcagccgt gattcccgca ataacagcgt ttcccatacg ggtcgacctg
    16981 cgtgtgacct atttccacga ggctactgct gcctgcgtgt agcagatgac catcggtaat
    17041 gacgcccgcc cccacgttgt gatcgataac cacctgaatc acatcgcgcg ccccgcgtga
    17101 ggcaccaaac aaggcctcag ccatcgtcca tgcgctgata tcatgctgaa tataaaccgg
    17161 tacgccggta tgctgctcca gcgcctcgcc gagcggcatc tcttttacat cctcgtagaa
    17221 cggcatgcga tgtacaatac cattttccgt atcaataatt cccggcaagg ttatggcaat
    17281 cgaagtcaga cgctcaagtt ttttctggtg gcggataaaa aactgatcga tatgggaaat
    17341 gatacgatcc agcaatggcg agtcatcttt taacgccagt tcctgcgact cctccactac
    17401 cagtttgctg ctcagatcgc gcaaagcaag gaaaatctcc ccgcgactaa tgcgcagaga
    17461 aagatagtgc caggcttcag tttcaaccac cagccccact gccggacggc cacggttccc
    17521 cgcttctttg atttccagct cttgcaccag gtgtgcttcg agcatctcac ggacaatttt
    17581 agtgatactg gcaggagcca gctgcgccag acgggaaaga tcgatacgcg agactggacc
    17641 aagctgatca atcaggcgat aaaccgcgcc cgcgttggtc tgctttattt gatcaatgtg
    17701 cccaggctga ttttcagcaa ccaccgcata ctccctatat tttcgcgctc cgaaataatc
    17761 tgtaggctat ggtgaagcac ttcaatacgt gtcgtcaaat ttttacttag gcatgtgatt
    17821 aacagcacat ttttcgggct ttttcgctga aatttccctc acctgagagc attcagcaga
    17881 tgaatacgaa agttacgcgc agccggactt tgttcatggt gtttcggcca gaccaaccac
    17941 atttcagaaa ccgcatcctc ttcagcaatc ggcacccagc gcatttcgtt gagctgaaca
    18001 cgtttaaatg acgcaggcaa aattgaaaca cccagaccag cggaaaccag accgatgatg
    18061 gtcattgcct cacccacctc ctgagtgatg acgggcgtca aattgtaacg tcgcatcagc
    18121 ccgagaatat cgtcatacag ccctgtccct acgtgcggat caaaaaagac aaagggttct
    18181 ttcgccagtt cagccagcgt tacactcggg ttatttgcca agggatgatc gtgcgggatc
    18241 atcgccataa gcggttcatg gacgatgact gcgtgttcaa gcgtctccgg taacgctgtg
    18301 ttacgcagca atcccatatc cagcgttcct tcaatgagcg gagcgatttg ctcgcgggtg
    18361 ttcatttcgc gggtttgtaa atggacatcg ggataatcac ggcgaaacag cgataacgta
    18421 tcggacaccg cccgaataaa aggagcggac gaagtaaaac caatgcgcaa ctcccccgct
    18481 tcaccctgat gcagtctttc agcgcgagcg gcagcgtcat ccatcataga caggatttgc
    18541 cgactatctg caagaaactg ttttcctgct gccgtcagca atacactgcg attagttcgt
    18601 gccagcagtc gggcaccaat ttgttgctcc agcgcctgaa tctgctgact tagcggcggt
    18661 tgcgaaatat tcaggcgggc agcggcgcgc ccgaaatgca gctcttctgc aacagcaaca
    18721 aagtaacgca gatgacgaag ttcaata
//
LOCUS       DAZFLD010000088        18618 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.088, whole genome shotgun sequence.
ACCESSION   DAZFLD010000088 DAZFLD010000000
VERSION     DAZFLD010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18618)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18618
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.088"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            8..925
                     /gene="yfbL"
                     /locus_tag="ACHKBL_002578"
     CDS             8..925
                     /gene="yfbL"
                     /locus_tag="ACHKBL_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family metallopeptidase"
                     /protein_id="HFO5819431.1"
                     /translation="MIFYQPWVRALPPTPRHASPEQLEKTVRYLTQTVHPRSADNIDN
                     LNRSAEYIKEVFISSGARVTAQDVPITGGPYKNIVADYGPADGPLIIIGAHYDSVSSY
                     ENDQLTYTPGADDNASGVAGLLELARLLQLQTPGIGVQLVAYASEEPPFFRSDEMGSA
                     VHAASLERPVKLMIALEMIGYYDSTPGSQDYPSPAMSWLYPDRGDFIAVVGRMQDINA
                     VRQVKAALLSSRDLSVYSMNAPGFIPGIDFSDHLNYWQHDIPAVMITDTAFYRNKQYH
                     LPGDIADRLNYQKMAQVVDGVTTLLYNSK"
     gene            1028..1531
                     /gene="yfbM"
                     /locus_tag="ACHKBL_002579"
     CDS             1028..1531
                     /gene="yfbM"
                     /locus_tag="ACHKBL_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbM family protein"
                     /protein_id="HFO5819432.1"
                     /translation="MGMIGYFAEIDSEKINQLLESTKKPLMDNIHDTLSGLRRLDIDK
                     RWDFLHFGLTGTSAFDPAKNDPLSRAVLGEHSLEDGIDGFLGLTWNQELAATIDRLEA
                     LDRSELRKQFSIKRLNEMEIYPGVTFSEELEGQLFASIMLDMEKLISAYRRMLRQGNH
                     ALTVIVG"
     gene            complement(1598..3055)
                     /gene="nuoN"
                     /locus_tag="ACHKBL_002580"
     CDS             complement(1598..3055)
                     /gene="nuoN"
                     /locus_tag="ACHKBL_002580"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HFO5819433.1"
                     /translation="MTITPQNLIALLPLLIVGLTVVVVMLSIAWRRNHFLNATLSVIG
                     LNAALVSLWFVGQAGAMDVTPLMRVDGFAMLYTGLVLLASLATCTFAYPWLEGYNDNK
                     DEFYLLVLIAALGGILLANANHLASLFLGIELISLPLFGLVGYAFRQKRSLEASIKYT
                     ILSAAASSFLLFGMALVYAQSGDLSFVALGKNLGDGMLNEPLLLAGFGLMIVGLGFKL
                     SLVPFHLWTPDVYQGAPAPVSTFLATASKIAIFGVVMRLFLYAPVGDSEAIRVVLAII
                     AFASIIFGNLMALSQTNIKRLLGYSSISHLGYLLVALIALQTGEMSMEAVGVYLAGYL
                     FSSLGAFGVVSLMSSPYRGPDADSLFSYRGLFWHRPILAAVMTVMMLSLAGIPMTLGF
                     IGKFYVLAVGVQAHLWWLVGAVVVGSAIGLYYYLRVAVSLYLHAPEQPGRDAPSNWQY
                     SAGGIVVLISALLVLVLGVWPQPLISIVRLAMPLM"
     gene            complement(3062..4591)
                     /gene="nuoM"
                     /locus_tag="ACHKBL_002581"
     CDS             complement(3062..4591)
                     /gene="nuoM"
                     /locus_tag="ACHKBL_002581"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HFO5819434.1"
                     /translation="MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALITMGLTLALSL
                     QLWLQGGYSLTQSAGIPQWQSEFDMPWIPRFGISIHLAIDGLSLLMVVLTGLLGVLAV
                     LCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
                     ASDGKTRITAATKFFIYTQASGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSSG
                     VEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSL
                     PLFPNASAEFAPIAMWLGVIGIFYGAWMAFAQTDIKRLIAYTSVSHMGFVLIAIYTGS
                     QLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFA
                     VATLGMPGTGNFVGEFMILFGSFQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKS
                     QIASQELPGMSLRELFMILLLVVLLVLLGFYPQPILDTSHSAIGNIQQWFVNSVTTTR
                     P"
     gene            complement(4755..6596)
                     /gene="nuoL"
                     /locus_tag="ACHKBL_002582"
     CDS             complement(4755..6596)
                     /gene="nuoL"
                     /locus_tag="ACHKBL_002582"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HFO5819435.1"
                     /translation="MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAALV
                     TAFIGVDFFANGEQAYSQPLWTWMSVGDFNIGFNLVLDGLSLTMLSVVTGVGFLIHMY
                     ASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTD
                     PKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFREMVELAPAHFADGNNMLMWA
                     TLMLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLMT
                     PEVLHLVGIVGAVTLLLAGFAALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFH
                     LMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYLCFLVGGAALSALPLV
                     TAGFFSKDEILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFIVFHGKEQIHAHAVKG
                     VTHSLPLIVLLILSTFVGALIVPPLQGVLPQTTELAHGSMLTLEITSGVVAVVGILLA
                     AWLWLGKRTLVTSIANSAPGRLLGTWWYNAWGFDWLYDKVFVKPFLGIAWLLKRDPLN
                     SMMNIPAVLSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALLMVLR"
     gene            complement(6593..6895)
                     /gene="nuoK"
                     /locus_tag="ACHKBL_002583"
     CDS             complement(6593..6895)
                     /gene="nuoK"
                     /locus_tag="ACHKBL_002583"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HFO5819436.1"
                     /translation="MIPLQHGLILAAILFVLGLTGLVIRRNLLFMLIGLEIMINASAL
                     AFVVAGSYWGQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMRG"
     gene            complement(6892..7446)
                     /gene="nuoJ"
                     /locus_tag="ACHKBL_002584"
     CDS             complement(6892..7446)
                     /gene="nuoJ"
                     /locus_tag="ACHKBL_002584"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HFO5819437.1"
                     /translation="MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFF
                     SLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAI
                     MLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGR
                     EERAGEVLSNRKDDSAKRKTEEHA"
     gene            complement(7458..8000)
                     /gene="nuoI"
                     /locus_tag="ACHKBL_002585"
     CDS             complement(7458..8000)
                     /gene="nuoI"
                     /locus_tag="ACHKBL_002585"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HFO5819438.1"
                     /translation="MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPVYLPPRY
                     RGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFC
                     GLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
                     KDKGEAENEAKPIDVKSLLP"
     gene            complement(8015..8992)
                     /gene="nuoH"
                     /locus_tag="ACHKBL_002586"
     CDS             complement(8015..8992)
                     /gene="nuoH"
                     /locus_tag="ACHKBL_002586"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HFO5819439.1"
                     /translation="MSWISPELIEILLTILKAVVILLVVVTCGAFMSFGERRLLGLFQ
                     NRYGPNRVGWGGSLQLVADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVP
                     VSPGWVVADLNIGILFFLMMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFL
                     GLSLMGVVAQAGSFNMTDIVNSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAE
                     QELADGYHIEYSGMKFGLFFVGEYIGIVTISALMVTLFFGGWQGPLLPPFIWFALKTA
                     FFMMMFILIRASLPRPRYDQVMSFGWKICLPLTLINLLVTAAVILWQAQ"
     gene            complement(8989..11715)
                     /gene="nuoG"
                     /locus_tag="ACHKBL_002587"
     CDS             complement(8989..11715)
                     /gene="nuoG"
                     /locus_tag="ACHKBL_002587"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HFO5819440.1"
                     /translation="MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYQNAEDTRGRLVMSCMTPASDGTFISIDDEEAKQFRESVVEWLMTNHPHD
                     CPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDLGPFISHEMNRCIACYRCVRY
                     YKDYADGTDLGVYGAHDNVYFGRPEDGTLESEFSGNLVEICPTGVFTDKTHSERYNRK
                     WDMQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLK
                     DRPRQPVQRRGDDFITLNAEQAMQGAADILRQSKKVIGIGSPRASVESNFALRELVGE
                     ENFYTGIAHGEQERLQLALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALA
                     VRQAVKGKAREMAAAQKVADWQIAAILNIGQRAKHPLFVTNVDDTRLDDIAAWTYRAP
                     VEDQARLGFAIAHALDNSAPAVDGIEPELQSKIDVIVQALAGAKKPLIISGTNAGSAE
                     VIQAAANVAKALKGRGADVGITMIARSVNSMGLGIMGGGSLEEALTELETGRADAVVV
                     LENDLHRHASATRVNAALAKAPLVMVVDHQRTAIMENAHLVLSAASFAESDGTVINNE
                     GRAQRFFQVYDPAYYDSKTVMLESWRWLHSLHSTLLSREVDWTQLDHVIDAVVAKIPE
                     LAGIKDAAPDATFRIRGQKLAREPHRYSGRTAMRANISVHEPRQPQDIDTMFTFSMEG
                     NNQPTAHRSQVPFAWAPGWNSPQAWNKFQDEVGGKLRFGDPGVRLFETSENGLDYFTS
                     VPARFQPQDGKWRIAPYYHLFGSDELSQRAPVFQSRMPQPYIKLNPADAAKLGVNAGT
                     RVSFSYDGNTVTLPVEIAEGLTAGQVGLPMGMSGIAPVLAGAHLEDLKEAQQ"
     gene            complement(11768..13105)
                     /gene="nuoF"
                     /locus_tag="ACHKBL_002588"
     CDS             complement(11768..13105)
                     /gene="nuoF"
                     /locus_tag="ACHKBL_002588"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HFO5819441.1"
                     /translation="MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTG
                     LSPDEIVNQVKDAGLKGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDR
                     LLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEVGLLGKNI
                     MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGAWGKPTCVNN
                     VETLCNVPAILANGVEWYQNISKSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILED
                     YAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSRLGTALAMAVDHEIN
                     MVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLG
                     PGKTFCAHAPGAVEPLQSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW"
     gene            complement(13102..13602)
                     /gene="nuoE"
                     /locus_tag="ACHKBL_002589"
     CDS             complement(13102..13602)
                     /gene="nuoE"
                     /locus_tag="ACHKBL_002589"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HFO5819442.1"
                     /translation="MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQ
                     KQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGY
                     QGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEAIPE
                     LLERYK"
     gene            complement(13605..15407)
                     /gene="nuoC"
                     /locus_tag="ACHKBL_002590"
     CDS             complement(13605..15407)
                     /gene="nuoC"
                     /locus_tag="ACHKBL_002590"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030964 - NADH dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HFO5819443.1"
                     /translation="MVNNMTDLTAQEPAWQTRDHLDDPVIGELRNRFGPDAFTVQATR
                     TGVPVVWIKREQLLEVGDFLKKLPKPYVMLFDLHGMDERLRTHREGLPAADFSVFYHL
                     ISIDRNRDIMLKVALAENDLHVPTFTKLFPNANWYERETWDLFGITFDGHPNLRRIMM
                     PQTWKGHPLRKDYPARATEFSPFELTKAKQDLEMEALTFKPEEWGMKRGTENEDFMFL
                     NLGPNHPSAHGAFRIVLQLDGEEIVDCVPDIGYHHRGAEKMGERQSWHSYIPYTDRIE
                     YLGGCVNEMPYVLAVEKLAGITVPDRVNVIRVMLSELFRINSHLLYISTFIQDVGAMT
                     PVFFAFTDRQKIYDLVEAITGFRMHPAWFRIGGVAHDLPRGWDRLLREFLDWMPKRLA
                     SYEKAALQNTILKGRSQGVAAYGAKEALEWGTTGAGLRATGIDFDVRKARPYSGYENF
                     DFEIPVGGGVSDCYTRVMLKVEELRQSLRILEQCLNNMPEGPFKADHPLTTPPPKERT
                     LQHIETLITHFLQVSWGPVMPANESFQMIEATKGINSYYLTSDGSTMSYRTRIRTPSY
                     AHLQQIPAAIRGSLVSDLIVYLGSIDFVMSDVDR"
     gene            complement(15501..16163)
                     /gene="nuoB"
                     /locus_tag="ACHKBL_002591"
     CDS             complement(15501..16163)
                     /gene="nuoB"
                     /locus_tag="ACHKBL_002591"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HFO5819444.1"
                     /translation="MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLND
                     MVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTC
                     FTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPP
                     RPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPD
                     EI"
     gene            complement(16179..16622)
                     /gene="nuoA"
                     /locus_tag="ACHKBL_002592"
     CDS             complement(16179..16622)
                     /gene="nuoA"
                     /locus_tag="ACHKBL_002592"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoA"
                     /protein_id="HFO5819445.1"
                     /translation="MSMSTSTEVIAHHWAFAIFLIVAIGLCCLMLVGGWFLGGRARAR
                     SKNVPFESGIDSVGSARLRLSAKFYLVAMFFVIFDVEALYLFAWSTSIRESGWVGFVE
                     AAIFIFVLLAGLVYLVRIGALDWTPARSRRERMNPETNSIANRQR"
     gene            complement(17252..18190)
                     /gene="lrhA"
                     /locus_tag="ACHKBL_002593"
     CDS             complement(17252..18190)
                     /gene="lrhA"
                     /locus_tag="ACHKBL_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LrhA"
                     /protein_id="HFO5819446.1"
                     /translation="MISANRPIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQ
                     QMQRLEQLVGKELFARHGRNKLLTEHGIQLLGYARKILRFNDEACSSLMFSNLQGVLT
                     IGASDESADTILPFLLNRVSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPST
                     FKALNLRTSPTHWYCAAEYVLQKGEPIPLVLLDDPSPFRDMVLATLNKADIPWRLAYV
                     ASTLPAVRAAVKAGLGVTARPVEMMSPDLRVLSGVDGLPPLPDTEYLLCYDPSSNNEL
                     AQVIYQAMESYHNPWQYSPMSAPEGDDSLLIERDIE"
BASE COUNT         4631 a         5164 c         4824 g         3999 t
ORIGIN      
        1 tacttttatt attttttacc aaccctgggt cagggcatta ccaccaacgc cccgacacgc
       61 tagtcctgag caattagaaa aaacagttcg ttatcttaca caaactgtgc atccacgtag
      121 cgccgacaat attgataatc tgaataggtc cgcagagtat ataaaagagg tctttatcag
      181 tagcggtgcc agagttaccg cgcaggatgt ccccattacg ggtggcccct acaaaaacat
      241 tgttgctgat tatggtcctg ccgatggacc gctgattatt attggtgcgc actatgactc
      301 tgtcagtagt tatgaaaacg atcaattgac ctatacgccg ggcgcggatg ataacgccag
      361 cggcgtggca ggattactcg aactggcgcg tttgttacag ctgcagactc cagggattgg
      421 cgtccaactg gtcgcctatg cgtcggaaga accgcccttc tttcgtagcg atgaaatggg
      481 gagcgcggta catgcagctt cgcttgagcg tccagtgaaa ttaatgatag cgctggagat
      541 gattggttat tacgactcaa cgcctggtag ccaggattac ccatccccgg caatgtcctg
      601 gctttatccc gatcggggag actttattgc cgtggtcggc agaatgcagg atatcaacgc
      661 cgttcgtcag gtaaaagcgg cattgttgtc atcccgggat ttatctgttt attctatgaa
      721 tgccccaggt tttattcccg gtattgattt ctctgatcac cttaattatt ggcaacacga
      781 tattcccgcc gtaatgatta ctgacaccgc tttttatcgt aataaacaat accacttgcc
      841 cggtgatatc gcagacagat taaattatca gaaaatggct caggtagtag atggagttac
      901 aaccttgtta tacaacagta aataataaaa atttctcgag gcggatccag aaaaagcaac
      961 atgcgcattt tattttcttt cgtctgtttt aagatgcgaa acatgaaatt caacggagga
     1021 aaaataaatg ggaatgattg gctattttgc agaaattgac tcagaaaaga ttaaccaact
     1081 gcttgagtct actaaaaaac ctttgatgga taatatacat gacacgcttt caggattacg
     1141 ccgactggat attgataaac gctgggattt tttacacttt ggcctgacgg gtacatctgc
     1201 atttgacccc gcgaagaatg acccgctaag tcgcgcagtg cttggtgaac acagcctaga
     1261 agatggcata gatggcttcc tgggattgac ctggaatcag gagttagccg cgacaatcga
     1321 ccgactggaa gctctggatc gaagtgagct gcgtaaacag ttttccatta agcgactcaa
     1381 tgaaatggag atttatccgg gagtaacctt ttcagaagag ttagaggggc aactctttgc
     1441 ttcgatcatg cttgatatgg aaaagctgat tagcgcgtat cgacgtatgc tgcgacaagg
     1501 gaatcatgcg ctgacggtta ttgttgggtg atggtgtcag ggcaccaaaa aaaaccgccg
     1561 aacatgtcgg cggctttctg acttacaaac tgactgctta catcagcggc attgctaacc
     1621 gcacaatgct aatcagcggt tgtggccata cacccagcac cagtaccaac agtgcagaga
     1681 tcagcaccac gataccgcct gcgctgtact gccagtttga tggtgcatcg cgacccggtt
     1741 gttccggagc gtgaaggtat aagctcaccg ccacgcgcag gtagtagtag aggccgattg
     1801 ccgaaccgac aactacggca cccaccagcc accacaagtg tgcctggaca ccgaccgcca
     1861 gcacgtagaa cttaccgata aagcccagcg tcattgggat accagccagt gaaagcatca
     1921 tcaccgtcat cactgccgcg aggatcggac gatgccagaa cagaccgcgg taagagaaca
     1981 gggaatcagc atccgggcca cgatacgggc tggacatcag gctgaccacg ccgaacgcgc
     2041 cgaggctgct gaacagataa cctgccaggt aaacccctac cgcttccatc gacatctcgc
     2101 cagtttgcag cgcaatcagc gccaccagca gatagccgag gtgagagata gatgagtaac
     2161 cgagcagacg tttgatattg gtctggctca gcgccatcag gttaccgaag atgatggagg
     2221 caaaggcgat aatcgccagc accacgcgaa tcgcttcgct gtcacccacc ggtgcgtaga
     2281 ggaacagacg catcaccaca ccgaagatag cgattttgct cgccgtcgcc aggaaagtgg
     2341 aaaccggcgc aggcgcgccc tggtatacgt ctggcgtcca caggtggaac ggcaccagag
     2401 agagtttgaa gccgaggccg acaatcatca ggccgaaacc tgccagcaac agcggctcgt
     2461 tgagcatacc gtcgccgagg tttttaccca gcgcgacaaa cgacaggtcg ccagactgcg
     2521 catacaccag cgccataccg aacagcagga aagaagacgc tgcggcagag agaatggtgt
     2581 atttgatact ggcttccagc gaacgtttct ggcggaaagc gtaaccgacc aggccaaaca
     2641 gcggcaaaga gatcagttcg ataccgagga acagagacgc cagatggttg gcattcgcca
     2701 gcaggatccc gcccagggcg gcaattaaca ccaacaggta gaactcgtcc ttgttgtcgt
     2761 tatagccttc aagccacggg taggcgaaag tacaggtggc gaggctcgcc aacaatacca
     2821 gcccggtgta aagcatggcg aaaccatcaa cgcgcatcag tggcgtaacg tccatagcgc
     2881 ccgcctggcc gacaaaccag agcgaaacca gcgccgcgtt aagcccaata accgagagcg
     2941 tagcgttgag gaaatgattg cgtcgccacg caatggagag catcaccacc accaccgtca
     3001 agccgacgat cagcaacggt agcagtgcga tcaggttttg tggagttatt gtcatggcga
     3061 tttacggcct tgtagtagta acggaattaa caaaccactg ctggatattg ccaatcgcgg
     3121 agtgcgaggt atccagaatc ggctgcggat agaagcccag cagtaccagc agcaccacca
     3181 gcaacaggat cataaacagc tcacgcagcg acatccctgg cagttcctgg ctggcaatct
     3241 ggcttttcgc tttaccgaag taagcgcgat gtaacatcgc cagcgaataa acagatgcaa
     3301 agaccagccc aaaggtagag ataacggtaa tcaccgggac aacctggaag ctgccgaaca
     3361 gaatcataaa ttcgccgacg aagttaccgg tgccaggcat cccaagcgtt gccaccgcaa
     3421 agaacagcga cagtgctggc agccatttca tcttgctcca cagaccgccc atcatgcgca
     3481 tgtcgcgggt atggatacgt tcataaagct gaccgcagag aataaacaga cccgccgccg
     3541 acaagccgtg cgcaatcatc tggattaccg cgccctggta ggccaactgg ctgccagtgt
     3601 agatagcaat cagcacgaag cccatgtggg aaaccgaggt gtaggcgatc agacgtttga
     3661 tatcggtctg ggcgaaggcc atccacgcac cgtagaagat gccgataaca cccagccaca
     3721 tagcgattgg cgcgaactct gccgacgcgt tcgggaacaa cggtagggag aaacgcagca
     3781 gaccgtaagc ggcggttttc agcaagatcc ccgcgaggtc aacggaaccg gcggttggag
     3841 cctgtgagtg cgcatccggc agccagccat gcagcggaac caccggcatt ttcaccgcaa
     3901 atgcgatgaa gaagcccagc atcaacagat attccacacc actggacatt ggcgtattca
     3961 acagctcttc atagttgaag gtccagacgc cggtcgcatt gtagtgaaca aaaaccagcg
     4021 ccaggatggc gatcaacatc accagaccac tcgcctgggt gtaaatgaag aacttggttg
     4081 ccgccgtgat acgcgtttta ccgtcagagg ctttatgccc ccacagtgcg atcaggaagt
     4141 acatcggcac cagcatcatt tcccagaaga agaagaacag gaacatgtcg atggcaagga
     4201 acacgccgat aacgccgccc aggatccaca tcaggttgag gtggaagaag ccctgatatt
     4261 tttcgatctc tttccacgaa cagagtaccg ccagcacacc gagcagaccg gtcagcacga
     4321 ccatcagcag cgacagcccg tcaatggcga gatggataga gataccaaaa cgcgggatcc
     4381 acggcatgtc gaattcagac tgccactgcg gaattccggc ggattgcgtc agtgaataac
     4441 cgccctgcaa ccacagttgc agcgacagcg ccagcgtcaa tcccatggtg atcagcgcga
     4501 tccagcgcgg caccttgacg ccaaagcgtt cggtctgcca gcacaggaag ccgccgataa
     4561 agggaattaa tattagccag ggtagtaaca tggcgatctt tattccttgt aaaagtcccg
     4621 tcaggaccgg attttcaacg aattctcacg acagaaaatt ctcttcggac tgggggtagt
     4681 gtcggatgca ccgcgaatgc ggcttatccg acctacgttc ttatatgccc aggggcaatc
     4741 ccacaatcct taactcaacg cagtaccatc aacagtgcca gcaccacgac cgcaccgatg
     4801 ctcatggatg ccacatacca gcgcagataa ccgttctcgc ttaacagcag acctttacct
     4861 gcaaagcggg aaaggacagc cgggatgttc atcattgagt tcagcggatc gcgtttcagc
     4921 aaccaggcaa tacccaggaa cggcttgacg aacactttgt cgtacagcca gtcaaagccc
     4981 caggcgttgt accaccaggt acccagcaga cggcccggcg cactgttggc gatggaggtc
     5041 accagagtac gtttacccag ccacagccag gcagccagca gaatgccgac caccgcgacc
     5101 acgccagagg taatttccag ggtcaacatg ctgccgtgcg ccagttccgt cgtttgcgga
     5161 agcacgccct gcagcggcgg tacaatcagt gcgccaacga aggtggaaag gatcagcagc
     5221 acaatcagcg gcaggctgtg agttacccct ttcacggcgt gagcgtgaat ttgttctttt
     5281 ccgtggaaga cgatgaaaat catacggaag gtgtagagcg aggtcataaa cgcaccgacc
     5341 agacctgcca ccatcagatt gatatgacca ttcgccatcg cacccgcgag gatctcatcc
     5401 ttactgaaga agcccgcagt gaccagcggc agtgccgaca gtgctgcgcc gcccaccagg
     5461 aagcagagat aaaccagcgg aatagattta cgcagaccgc ccatcttgaa gatgttctgt
     5521 tcgtgatggc aggccagaat gacggaaccg gatgccagga acagcagcgc tttaaagaac
     5581 gcgtgggtca tcaagtggaa aatcgccgca tcccatgcct gcacgccaag cgcgaggaac
     5641 atgtagccaa tctggctcat ggtagagtaa gcgagaacac gtttgatatc ggtctgtacc
     5701 agcgcggcaa aaccggccag cagcagcgta accgccccga caatgcccac cagatgcaga
     5761 acttccggcg tcatcaggaa caggccgtgg gtacgagcga tcaggtagac acccgcggtt
     5821 accatcgttg cggcgtggat cagcgcggag acaggcgtcg ggcccgccat cgcgtcggca
     5881 agccatgtct gcaacggcaa ctgcgcagat ttaccgaccg caccgcccag cagcatcagc
     5941 gtcgcccaca tcagcatgtt attgccgtca gcaaagtgcg ctggtgccag ttccaccatt
     6001 tcgcggaagt tcagggtgcc cagttcgttg taaagaatga acagcgcgaa agcgaggaac
     6061 acgtcaccca cacgggtcac gacgaacgct ttcattgccg ctgcgccatt cttcggatcg
     6121 gtgtaataga aaccgatcag cagataggag cacaggccca cgccttccca gccgaggtac
     6181 atcaacagta ggttatcggc gagaaccaga actaccatgc tggcgatgaa caggttggtg
     6241 taagcgaaga agcgagagta gccctcttct ccgcgcatat accaggaggc atacatgtga
     6301 ataaggaaac ccacaccagt gaccactgag agcatggtca gcgacaggcc gtccagcacc
     6361 agattgaaac caatgttaaa gtcgcctacc gacatccacg tccacagcgg ctggctgtat
     6421 gcctgctcac cgttagcgaa gaagtcaacg ccgataaagg cggttaccag cgccgccagg
     6481 cccacagagc caacgccgac gatcgccgag acgttttcag accagcgccc acgggagaac
     6541 gccagcagga cgaagccaat caatggcaaa ataatggtta aggcaagcat gttcatccgc
     6601 gcatctcact tactgaatcg atgttcaggt tctggcgacg acggtgaagt tgcagcagca
     6661 gcgcaaggcc gatactcgct tccgccgccg cgaggctgat ggcgagaatg tacatcacct
     6721 gaccgtcggt ctggccccag tagcttccgg cgaccacaaa ggccagcgca gaggcgttaa
     6781 tcatgatttc cagaccaatc agcataaaca gcagattgcg acggataacc agaccggtta
     6841 agccaagaac gaataagatt gccgcgagga tcagtccatg ttgtaagggg atcatgcgtg
     6901 ctcctccgtt tttcttttcg cgctgtcgtc tttacgattg ctcagcactt cacccgcacg
     6961 ctcttcacga ccgacgtgga aggccacaac cagacccgcg agcagcagca tagaagccag
     7021 ttccaccgcc agtacgtaag gcccgaacag cgtaataccc actgctttag cactgattgg
     7081 cgtaccgtcg ataccctgat cattaacacc aaggatggcg taaacaatca ccaccagcat
     7141 gatggccgac aaaatcgccg gaccaatcca cacctgcggt ttcagccact ggcgttcctg
     7201 ttcgatttct gaaccgccca ggttgagcat catcaccacg aacacgaaca gcaccataat
     7261 ggcacccgcg tagacgataa tttccagcgc accggcgaag taagcgccca gtgagaagaa
     7321 caccccagaa atcgccagca gcgaaataat caggtacagc agcgcgtgta ccggattggt
     7381 atgggtgatc actcgcaagg tcgcaagtat ggctatcagg ccacagatat aaaaagcgaa
     7441 ctccattgcc cctctcctta cggtaacagg ctcttgacgt cgataggctt ggcttcgttc
     7501 tctgcttcgc ccttatcttt gccgtcgatt gccatacctg ccatccggta gaagttatat
     7561 tccgggtatt tgcccggacc ggagatcagc agatcctctt tctcgtaaac cagatcctgg
     7621 cgcttgtatt cccccatttc gaaatccggg gttaactgaa tcgccgtggt cggacaggct
     7681 tcttcgcaca gaccacagaa aatgcagcgt gagaagttga tgcggaaaaa ttccggatac
     7741 cagcgaccgt ctttggtttc tgctttttgc agcgagatac agccgaccgg gcaggctacc
     7801 gcgcagaggt tacaggctac gcaacgctct tcgccgtccg gatcgcgggt cagaacgata
     7861 cgaccacgat aacggggcgg cagatagacc ggctcttccg ggtacattcg cgtttcgcgt
     7921 ttggcgaacg cgtgcaggcc gatcatccag atactacgaa cctgggtgcc gaaacctact
     7981 aacaattctt ttaaggtcat ggtcttattg ccccttattg cgcctgccag agaatgacag
     8041 ccgccgttac cagcaagttg atcagcgtca gcggcaggca gattttccag ccgaaggaca
     8101 ttacctggtc ataacgcgga cgcggtaacg acgcacgaat caaaatgaac atcatcataa
     8161 agaacgcggt tttcagcgcg aaccagatga atggcggtaa caacgggcct tgccagccac
     8221 cgaagaacag cgtcaccatc agtgcagaga tggtcacaat cccgatgtat tcgcccacga
     8281 agaacagacc gaacttcatg ccggaatatt caatgtggta accatccgcc agttcctgct
     8341 cggcttccgg ctggtcaaac gggtgacggt gacacaccgc cacgcccgcg atggcaaagg
     8401 taataaaacc aaagaattgc gggatgacgt tccacacatg cgcctggctg ttgacgatgt
     8461 cggtcatgtt gaatgaaccg gcctgcgcca ccacgcccat cagggaaagc ccgaggaaca
     8521 cttcgtagct cagggtctgc gcagaagcac gcatcgcacc cagcagcgag tatttgttgt
     8581 tacttgacca gcccgcaaac agcaccgcgt aaaccgccag accggccatc atcaggaaga
     8641 acaaaatccc gatgttcagg tcggcaacca cccaacccgg actgactggc acaatcgcaa
     8701 aggccagcag cagcgaggta aaggcaatca tcggtgccag ggtaaagatg acgcgatccg
     8761 agaatttcgg gatccagtct tctttaaaga acatcttgat catgtccgcg accagctgga
     8821 gcgaaccgcc ccagccaaca cggttaggtc cgtaacggtt ctggaacaga cccagcaggc
     8881 gacgttcgcc aaagctcatg aatgccccac aggtgacaac caccagcagg atcaccaccg
     8941 ctttgaggat ggtcagcagg atctcaatca gttccggtga tatccaactc attgttgtgc
     9001 ctccttgaga tcctcaagat gcgcgccagc cagcaccgga gcaatgccgg acatacccat
     9061 cggcaagccc acctgccctg ccgtcagtcc ttcggcgatt tcaaccggca gcgtgaccgt
     9121 gttgccatcg taactaaagg agacgcgtgt acccgcgttc acacccaact tcgcggcatc
     9181 cgctgggttg agtttgatgt acggctgcgg catacggctc tggaagaccg gagcacgctg
     9241 tgacaattca tcgctaccaa acaggtggta gtacggcgcg atacgccatt tcccgtcctg
     9301 cggctggaag cgtgccggta cgctggtgaa gtaatccaga ccattttcgc tggtttcaaa
     9361 cagacgcacg cccggatcgc caaagcgcag tttgccgccc acttcgtcct ggaatttgtt
     9421 ccacgcctgc ggggagttcc agcctggcgc ccaggcaaac ggcacttgcg aacggtgcgc
     9481 ggtcggctgg ttgttacctt ccatcgagaa ggtgaacatg gtgtcaatat cctgcggctg
     9541 acgcggctca tgaacgctga tattggcgcg catggcggta cgaccgctgt aacggtgcgg
     9601 ttcacgggcc agtttctgcc cacgaatacg gaatgtcgca tccggcgcag catctttgat
     9661 acccgccagt tccgggattt tcgccacaac agcgtcaatc acatggtcga gctgcgtcca
     9721 gtccacttca cggctcagca aggtgctgtg cagcgagtgt aaccaacgcc agctttccag
     9781 catgacagtt ttgctgtcgt aataagccgg atcgtaaacc tggaagaaac gttgggcacg
     9841 gccttcgttg ttaatcaccg taccgtcgct ttcagcaaag ctggcagcag aaagtaccag
     9901 atgggcgttt tccataatcg ctgtgcgttg atggtcaacc accatcacca gcggcgcttt
     9961 agccagcgca gcattcacgc gggtagcgga agcgtgacga tgcagatcgt tttccagcac
    10021 caccaccgcg tcggcgcgtc cggtttccag ttcggttaac gcttcttcaa gcgatccacc
    10081 gcccataatg cccagcccca tgctgttgac ggaacgggca atcatggtga taccgacgtc
    10141 agcgccgcga cctttcaggg ctttcgccac gttagccgca gcctgaatca cttcagcgct
    10201 accggcgttc gtcccggaga taatcaacgg tttcttcgca cctgccagtg cctgcacgat
    10261 gacgtcgatt ttgctttgca gctcaggttc aataccgtca accgctggcg cggagttatc
    10321 cagcgcatgg gcgatggcaa aacctaaacg cgcctgatct tcaaccggtg cgcggtaagt
    10381 ccacgccgcg atatcatcca gacgggtgtc atcaacgttg gtaacaaaca gcggatgctt
    10441 cgcacgttgg ccgatgttga ggattgccgc aatctgccag tcagccactt tctgtgctgc
    10501 cgccatttcg cgcgctttac ctttcaccgc ctgacgaact gccagcgcga cgcgcgcgcc
    10561 ggtctgggta acgtcttcgc ccagcaccag taccgcatcg taagattcga tttcgcgcag
    10621 tgccggcgta taaatgccgc cttcacgcag cactttcagc gccagttgca gacgttcctg
    10681 ctcaccgtga gcgataccgg tgtagaagtt ttcttcgccc accagctcac gcagcgcaaa
    10741 gttgctttcc acgctggcac gcggagaacc aatgccgatc actttcttcg actgacgcag
    10801 aatatctgcc gcgccctgca ttgcctgttc ggcgttgagg gtaatgaaat catcgccacg
    10861 acgctgtact ggctgacgcg gacgatcttt caggttgacg taaccgtaac cgaaacgacc
    10921 acggtcgcag aggaagtagt ggtttaccgt accgttgtaa cggttttcga tacgacgcag
    10981 ttcgccgtaa cgctcgccgg ggctgatgtt acagccgatg gaacattgct ggcagatgct
    11041 cggcgcaaac tgcatatccc atttacggtt gtaacgctcg gagtgcgttt tgtcggtgaa
    11101 tacgccggtc gggcaaatct cgaccagatt accggaaaat tcgctttcca gcgtgccgtc
    11161 ttccgggcga ccgaagtaga cgttgtcgtg cgcaccgtaa acgcccaggt ccgtgccgtc
    11221 agcgtaatct ttgtagtaac gcacgcagcg gtagcaggcg atgcagcggt tcatttcgtg
    11281 agagatgaat ggccccaaat cctgattacg gtgggtacgt ttggtgaaac ggtagcgacg
    11341 gaagctgtgt ccagtcatca cagtcatatc ctgaagatgg cagttaccgc cctcttcaca
    11401 taccggacag tcgtgcgggt ggttggtcat taaccactcg accacacttt cacggaactg
    11461 tttcgcttct tcatcgtcaa tggaaataaa ggtgccatcg gaagccggtg tcatacagga
    11521 catcaccagg cgaccacgcg tgtcttccgc gttttggtat tgcttcaccg cacactggcg
    11581 gcaagcaccg acgcttccca gcgccggatg ccagcaaaag taaggaatat caaggcccag
    11641 agacagacaa gcttccagca agttgtccgc tccgttgacc tcgtattctt tgccgtctac
    11701 atgaattgta gccattagca tgcttccagt tttctcagtc agagactgag cgttaatcga
    11761 aattcggtta ccagcgctct ttcagcaggt tcggctgaat cccattaatc aaatgggtat
    11821 tgctgaacgg ctgtttgatt cccgcctcaa attcttcgcg gaaatatttg atggcgctct
    11881 gtaacggctc cactgcacca ggtgcgtggg cacagaaagt tttacccggg cctaagaatc
    11941 gacacagttg ctcaagtgtt tcgatatcgc ccggctgacc ttcgccacgc tccagcgcac
    12001 gcagaatttt cacgctccac ggcagaccgt cgcggcacgg cgtacaccag ccacaggact
    12061 cacgggcgaa aaactcttcc aggttacgca ccagcgacac catgttgatc tcatggtcaa
    12121 ccgccatcgc cagcgccgta cccagacggc ttcccgcttt accgatactt tcgaattcca
    12181 tcggcagatc aaggtgcgct tcggtcagga agtcggtgcc agcgccgcct ggctgccagg
    12241 ctttgaattt cagaccgtca cgcataccac cggcgtaatc ttcgaggatc tcgcgtgcgg
    12301 tggtgccgaa tggcagttcc cacaggcccg gatttttcac ccgaccggag aagcccatca
    12361 gcttggtgcc agcatcttta cttttcgaga tgttctgata ccactccacg ccgttagcga
    12421 ggatcgccgg aacgttacac agggtttcga cgttattgac acaggtcggt ttgccccatg
    12481 caccggaggt tgccgggaat ggaggcttcg agcgagggtt agcgcgacgc ccttccaggg
    12541 agttgattaa cgctgtttct tcaccgcaga tgtagcgccc agccccggta tggacgaaca
    12601 attcgaagtc gaaacctgtt cccataatgt ttttgccaag cagacccact tcggtggctt
    12661 cggcaatggc acggcgcaga ttcactgccg cttcgatata ctcgccacgc aggaagatgt
    12721 agccacggta cgctttcagc gcaaacgcgg agatgagcat accttccacc agcaggtgcg
    12781 gcagttgttc catcagcagg cggtctttat aggtgcccgg ctccatttca tcggcgttac
    12841 acagcaggta acggatgttc atggattcgt ctttcggcat caggctccat ttcaggccgg
    12901 tagaaaagcc cgcgccgccg cgccctttca gaccagcgtc ttttacctga ttaacgattt
    12961 cgtccggaga cagcccggtc agcgccttac gcgcgccttc gtaaccgttt ttgctgcgat
    13021 attcgtccag ccacactggc tgtttgtcat cgcgcagacg ccaggtcagc ggatgcgttt
    13081 cgggagtacg gataatgttt ttcatttata ccgctccagc agttcaggga tcgcttccgg
    13141 ggtcagatgc gcgtgagtgt cctcatcgat catcatgttt ggccctttgt cgcagttccc
    13201 caggcagcaa gttggcagca gcgtaaagcg gccatcaaat gtcgtttgcc ctggtttgat
    13261 gttcagcttt ttctccagcg ccgcctgaat accctggtaa ccgttgatgt gacagaccac
    13321 gctgtcgcaa taacggatca catggcgacc aaccggctgg cggaagatct ggctatagaa
    13381 cgttgccacg ccttcgacgt cgctcgccgg aatacccagc acatcggcga tcgcgtggat
    13441 cgcgccatcc ggcacccagc cacgctgctt ctgaacgatt ttcagcgctt caatggacgc
    13501 cgcacgcggg tcttcgtagt ggtgcatctc gtgctcaatc gcttcacgct ctgccgcact
    13561 cagctcaaaa gcctcggttt gtggttgttg attctcgtgc ataattagcg gtccacatct
    13621 gacataacaa aatcgatact gcccagataa acaatcaggt cagacaccag gctgccgcgg
    13681 atcgccgccg gaatttgctg caaatgcgca taactcggcg tacggatacg ggtgcgataa
    13741 ctcatggtgc taccgtcgct ggtcaggtag taactgttga tccccttggt cgcctcaatc
    13801 atctggaaag attcattggc tggcatcacc ggaccccacg acacttgcag gaagtgggtg
    13861 atcagggttt cgatatgttg cagcgtgcgc tctttcggcg gcggcgtggt cagcggatga
    13921 tccgctttga acgggccttc cggcatgttg ttgaggcact gctcaagaat gcgcagactc
    13981 tggcgcagct cttccacttt cagcattacg cgggtgtagc agtcagaaac accaccaccc
    14041 accgggattt caaagtcgaa gttttcatag ccagaataag gacgcgcctt acgcacgtcg
    14101 aagtcgatcc cggtggcgcg caggcccgcg ccagtggtgc cccactccag cgcctctttc
    14161 gcgccatagg cggcaacgcc ctgggaacga cctttcagaa tggtgttttg cagcgccgct
    14221 ttctcgtaag acgccagacg tttcggcatc cagtcgagga actcacgcag caggcgatcc
    14281 cagccacgcg gcaggtcgtg cgctacaccg ccaatacgga accacgccgg gtgcatacga
    14341 aaacccgtga tcgcttccac cagatcgtaa attttctgac gatcggtaaa ggcgaagaac
    14401 actggcgtca tcgcgccgac gtcctgaata aaggtggaga tatacagcag gtgactgttg
    14461 atgcggaaca gttcggagag cattacgcga atgacgttaa cgcgatccgg cacggtgatc
    14521 ccggccagtt tctctaccgc cagcacgtaa ggcatttcgt taacgcagcc gccgaggtat
    14581 tcgatacggt cggtatacgg aatgtagctg tgccaggact ggcgttcgcc cattttctcc
    14641 gcaccacggt ggtggtagcc gatgtctggt acgcagtcga caatttcttc gccatcaagc
    14701 tgcaaaacga tgcggaaagc cccgtgcgcc gacgggtggt tcgggccgag gttgaggaac
    14761 atgaagtcct cgttttcggt gccgcgcttc atcccccact cttccggttt gaaggtcagg
    14821 gcttccatct ccaggtcctg tttggctttg gtcagctcaa acggcgagaa ttcggtagcg
    14881 cgcgccggat aatctttacg cagcgggtga cctttccagg tttgcggcat catgatgcgg
    14941 cgcaggttcg ggtgaccgtc gaaagtaatg ccaaacagat cccaggtttc acgctcatac
    15001 cagttagcgt tcgggaacag tttggtgaag gtcggtacgt gcaggtcgtt ttctgccagc
    15061 gccaccttca gcatgatgtc gcggttacga tcgatagaaa tcagatggta gaaaacggaa
    15121 aaatccgcgg caggtaaccc ttcgcggtgt gtgcgcagac gttcgtccat gccgtgtaag
    15181 tcaaacagca tgacgtaagg tttcggcagt ttctttaaga aatcgcccac ttccagtaat
    15241 tgttcacgct tgatccacac aacgggaacc ccggtgcgag tcgcctgaac agtaaaggca
    15301 tccggcccaa aacggttgcg cagttcgcca atcaccggat catcaagatg atcgcgggtc
    15361 tgccaggcgg gttcttgcgc ggttaagtcg gtcatattgt tcaccattgc aaatggtccg
    15421 tggtgactgt cggcgcagta agcttcgcga aatagattta ggaataagcg aagaaaaatt
    15481 cctttgccga caggcgcaaa ttaaatctcg tcaggtgtac gcaggttggt tacggcaatg
    15541 cgttcaccgc gcttgcgttc gcgctctgat tgcatattgg cgcgataaac gccctgatcg
    15601 ccaaccaccc aggagagcgg acgacgttct ttaccgatag attcctgcaa cagcatcagt
    15661 gcctgcatgt acgcttcagg acgcggaggg cagcccggga tatacacatc aaccgggatg
    15721 aatttatcga cgccctgcac aacggaataa atatcgtaca taccgccgga gttggcacag
    15781 gcacccattg agataaccca ttttggttcc agcatctggt catacagacg ctgaataacc
    15841 ggtgccattt ttgtaaagca ggttcctgca acgaccatca ggtcagcctg acgcggcgaa
    15901 gctcgcaata cttctgcgcc aaaacgcgcc acgtcatgca ccgcggtaaa cgaagtcacc
    15961 atctcaacgt aacagcagga aagaccgaag ttatacggcc aaattgagtt tttacgaccc
    16021 cagttaacca tgtcattgag cttgcccata aacacgtttt tattaacttc ttgctccaga
    16081 gggtcggtta cgatctcctg cttttgcagg gggtaacggt cgttctcacc gttgggatct
    16141 atgcgggtga gcgtataatc catcttaatg cctcgcggtt agcgttgacg attagcgata
    16201 ctgttcgttt ccgggttcat acgctcgcgg cgtgaacgcg cgggcgtcca gtccagcgcg
    16261 ccaatacgca ccagataaac cagacctgcc agtaacacaa aaataaaaat tgcagcttcc
    16321 acaaagccta cccagccgct ttcgcggata gaggttgacc atgcgaacag atacagcgct
    16381 tcaacgtcga agataacgaa gaacatggcc accagataaa acttggcgga caggcgtaag
    16441 cgggcggagc cgaccgagtc gataccggat tcaaacggaa cgtttttcga ccttgcgcgt
    16501 gcgcgaccgc ctaaaaacca accgcctacc agcatcaggc aacacaggcc aatggcaacg
    16561 ataagaaaga tagcgaatgc ccagtgatga gcgatgactt cagtggatgt tgacatactc
    16621 attgcttact catcaaaagt agcgccagat tcactgctct tcacggcaga tggacgccac
    16681 atcgattcat ggggaggaat aaaaaaaacc ttacaatcac tgtagaaatt cttttataca
    16741 gctaattgat gtggtctttt actcctttct ataacctttt gtcaacttta acaaaagttt
    16801 cttcacatta gtttacataa tatcaacacc attagcattt aatgcccttt caccccagat
    16861 ccttgacgac tccaggataa ttagatgttg ttgaatcgtg tccgttgtga agcaatggaa
    16921 aaaatacggg tctattttga caggaatttg tgtcgattcc tccccccaaa agagagtatt
    16981 ttcttgatct gtgacacgct tttgtcattc cataacaaaa acgcagcaac aaatttacgt
    17041 attttttaac atcattgtag caggtgattt ttttcaggcg attatttgtg cgttcgggac
    17101 gtgaatctct ggtgattgaa aaatgaacag ttttgtacgt tctgcacgat acgaggaagg
    17161 ctttttacta aaaaaaagcc gctggggttt aaaacacccc cagcggctcg tttttacact
    17221 attgtctcag aaattatcta tggtccgtcg attactcgat atccctttca atcaacaatg
    17281 agtcatcgcc ttccggagca gacattgggc tgtattgcca cggattatgg tagctctcca
    17341 tcgcctgata gatcacctgg gccagctcat tattactgga cgggtcatag cacagcaaat
    17401 attcggtatc gggtagcggc ggtaagccat ctactccact cagcacacgc aggtctggac
    17461 tcatcatctc gaccggcctt gccgtcacgc caagaccggc ttttactgcc gcacgaactg
    17521 ccggaagcgt cgaggcgaca taagccagtc gccatggaat atctgcttta ttaagcgtcg
    17581 ccagcaccat atcgcgaaac gggctaggat catccagcaa tacaagaggg atcggctcgc
    17641 ctttttgcaa tacgtattcc gctgcgcagt accagtgtgt tggcgaggta cgcaggttca
    17701 atgccttaaa cgttgaagga cgatgggtgg ttaccatcaa atccacttcc tgggattcca
    17761 gcatttctgc catataggca ttacgtttaa cgcggacatc cagcgctagt ttcggataaa
    17821 ccgaactcac gcgatttaac aggaaaggta agatcgtatc ggcagattca tctgaagcac
    17881 cgatagttaa cacgccctga agattactga acattaatga tgagcaggcc tcatcattaa
    17941 aacgcagaat ttttctggcg taaccaagaa gttgaatgcc atgttcagtt aacagtttgt
    18001 tgcgaccgtg acgagcgaac agttctttcc caacgagttg ttccagacgc tgcatttgct
    18061 gacttacggc ggactgagta cgacacacag cggcagctgc ggcagcaaaa gtgttcagat
    18121 cggcaacagc aacaaatgtt ctcagcagat cgaggtcgag gttaattatc ggacgatttg
    18181 cacttatcat atattatcac ttactggcgg ctcatactga gctggttaat gctgtgcaca
    18241 cacaaacaag caattccatt tgtaatgtgc ctccctggca gtttcatccg gaacccggac
    18301 gaaagtaaaa atgcatatga gttgcactaa aaaagcgact cacattgttc cgttataatg
    18361 cctgaagtag atcacagaat atatcttcag gaatcgcata tctattaagt tactcactct
    18421 tttgtattta tgacatgcgc gtgtttgtat aaatgtaaat gtgagtcctt gttccactct
    18481 cgtgcagcat cgctggtcat acgcgaacac gtacaaacag caatggtgag gcggcatcaa
    18541 gagcagggat ccgtcattta tgcgagcatt ttaccccaaa aaccttttat ttataagggt
    18601 cattgcgaat tatctgat
//
LOCUS       DAZFLD010000089        18611 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.089, whole genome shotgun sequence.
ACCESSION   DAZFLD010000089 DAZFLD010000000
VERSION     DAZFLD010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18611
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.089"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            252..1160
                     /gene="gltI"
                     /locus_tag="ACHKBL_002594"
     CDS             252..1160
                     /gene="gltI"
                     /locus_tag="ACHKBL_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein GltI"
                     /protein_id="HFO5819447.1"
                     /translation="MQLRKPATAILALALSAGLAQADDAAPAAGSTLDKIAKNGVIVV
                     GHRESSVPFSYYDNQQKVVGYSQDYSNAIVEAVKKKLNKPDLQVKLIPITSQNRIPLL
                     QNGTFDFECGSTTNNAERQKQAAFSDTIFVVGTRLLTKKGGDIKDFADLKGKAVVVTS
                     GTTSEVLLNKLNEEQKMNMRIISAKDHGDSFRTLESGRAVAFMMDDALLAGERAKAKK
                     PDNWEIVGKPQSQEAYGCMLRKDDPQFKKLMDDTIAQVQTSGEAEKWFDKWFKNPIPP
                     KNLNMNFELSDEMKALFKEPNDKALN"
     gene            1330..2070
                     /gene="gltJ"
                     /locus_tag="ACHKBL_002595"
     CDS             1330..2070
                     /gene="gltJ"
                     /locus_tag="ACHKBL_002595"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltJ"
                     /protein_id="HFO5819448.1"
                     /translation="MSIDWNWGIFLQQAPFGNTTYLGWIWSGFQVTIALSICAWIIAF
                     LVGSFFGILRTVPNRFLSGLGTLYVELFRNVPLIVQFFTWYLVIPELLPEKIGMWFKA
                     ELDPNIQFFLSSMLCLGLFTAARVCEQVRAAIQSLPRGQKNAALAMGLTLPQAYRYVL
                     LPNAYRVIVPPMTSEMMNLVKNSAIASTIGLVDMAAQAGKLLDYSAHAWESFTAITLA
                     YVLINAFIMLVMTLVERKVRLPGNMGGK"
     gene            2070..2744
                     /gene="gltK"
                     /locus_tag="ACHKBL_002596"
     CDS             2070..2744
                     /gene="gltK"
                     /locus_tag="ACHKBL_002596"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
                     /protein_id="HFO5819449.1"
                     /translation="MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVM
                     RLSSFAPVAWFAKAYVNVFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKNDIRLISAMV
                     AFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
                     QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSY
                     LKRRTA"
     gene            2744..3469
                     /gene="gltL"
                     /locus_tag="ACHKBL_002597"
     CDS             2744..3469
                     /gene="gltL"
                     /locus_tag="ACHKBL_002597"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter ATP-binding
                     protein GltL"
                     /protein_id="HFO5819450.1"
                     /translation="MITLKNVSKWYGHFQVLTDCSTEVKKGEVVVVCGPSGSGKSTLI
                     KTVNGLEPVQQGEITVDGIVVNDKKTDLAKLRSRVGMVFQHFELFPHLSIIENLTLAQ
                     VKVLKRDKAPAREKALKLLERVGLSAHANKFPAQLSGGQQQRVAIARALCMDPIAMLF
                     DEPTSALDPEMINEVLDVMVELANEGMTMMVVTHEMGFARKVANRVIFMDEGKIVEDS
                     PKDAFFDDPKSDRAKDFLAKILH"
     gene            3587..4522
                     /gene="rihA"
                     /locus_tag="ACHKBL_002598"
     CDS             3587..4522
                     /gene="rihA"
                     /locus_tag="ACHKBL_002598"
                     /EC_number="3.2.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050263 - ribosylpyrimidine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-specific ribonucleoside hydrolase
                     RihA"
                     /protein_id="HFO5819451.1"
                     /translation="MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEK
                     TLRNVLRMLTLLNRTDIPVAGGAVKPLMRNLIIADNVHGESGLDGPALPEPTFAPQNC
                     TAVELMAKTLCESEEPITIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWT
                     PAAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHVEDTERFRAIGNPVSTIVAE
                     LLDFFLEYHKDEKWGFVGAPLHDPCTIAWLLKPELFTTVERWVGVETQGKYTQGMTVV
                     DYYYLTGNKPNATVMVDVDRQGFVDLLADRLKFYA"
     gene            complement(4567..5049)
                     /gene="ybeL"
                     /locus_tag="ACHKBL_002599"
     CDS             complement(4567..5049)
                     /gene="ybeL"
                     /locus_tag="ACHKBL_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon-containing protein"
                     /protein_id="HFO5819452.1"
                     /translation="MNKVAQYYRELVASLSERLRNGERDIDALVEQARERVIKTGELT
                     RTEVDELTRAVRRDLEEFAMSYEESLKEESDSVFMRVIKESLWQELADITDKTQLEWR
                     EVFQDLNHHGVYHSGEVVGLGNLVCEKCHFHLPIYTPEVLTLCPKCGHDQFQRRPFEP
                     "
     gene            5284..7866
                     /gene="leuS"
                     /locus_tag="ACHKBL_002600"
     CDS             5284..7866
                     /gene="leuS"
                     /locus_tag="ACHKBL_002600"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008456064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HFO5819453.1"
                     /translation="MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYP
                     SGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTY
                     DNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
                     NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKT
                     MQRNWIGRSEGVEITFNVNDYDNTLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNP
                     ELAAFIDECRNTKVAEAEMATMEKKGVDTGFKAVHPLTGEEIPVWAANFVLMEYGTGA
                     VMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDH
                     EAAFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQL
                     PVILPEDVVMDGITSPIKADPEWAKTTVNGMPALRETDTFDTFMESSWYYARYTCPEY
                     KEGMLDSKAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQ
                     GMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSK
                     NNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTA
                     KGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAP
                     TDGEQDRALMQEALLAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVED
                     STLVVVQVNGKVRAKITVPVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLN
                     LVVG"
     gene            7881..8462
                     /gene="lptE"
                     /locus_tag="ACHKBL_002601"
     CDS             7881..8462
                     /gene="lptE"
                     /locus_tag="ACHKBL_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HFO5819454.1"
                     /translation="MRYLATLLLSLAVLITAGCGWHLRDTTQVPSTMKVMILDSGDPN
                     GPLSRAVRNQLRLNGVELLDKETTRKDVPSLRLGKVSIAKDTASVFRNGQTAEYQMIM
                     TVNATVLIPGRDIYPISAKVFRSFFDNPQMALAKDNEQDMIVKEMYDRAAEQLIRKLP
                     SIRAADIRSDEEQTSTTTDTPATPARVSTTLGN"
     gene            8462..9493
                     /gene="holA"
                     /locus_tag="ACHKBL_002602"
     CDS             8462..9493
                     /gene="holA"
                     /locus_tag="ACHKBL_002602"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HFO5819455.1"
                     /translation="MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAIRQVAAV
                     QGFEEHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTG
                     LLHDDLLLIVRGNKLSKAQENAAWFTALANRSVQVTCQTPEQAQLPRWVAVRAKQLNL
                     ELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWV
                     DALLMGKSKRALHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHMPLRALFDKH
                     RVWQNRRGMMGEALNRLSHPQLRQAVQLLTRTELTLKQDYGQSVWAELEGLSLLLCHK
                     PLADVFIDG"
     gene            9495..10136
                     /gene="nadD"
                     /locus_tag="ACHKBL_002603"
     CDS             9495..10136
                     /gene="nadD"
                     /locus_tag="ACHKBL_002603"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HFO5819456.1"
                     /translation="MKSLQALFGGTFDPVHYGHLKPVETLANLIGLTRVTIIPNNVPP
                     HRPQPEANSMQRKHMLELAIADKPLFTLDERELKRNAPSYTAQTLKEWRQEQGPDVPL
                     AFIIGQDSLLTFPTWYEYETILDNAHLIVCRRPGYPLEMAQPQYQQWLEDHLTHNPED
                     LHLQPAGKIYLAETPWFNISATIIRERLQNGESCEDLLPEPVLTYINQQGLYR"
     gene            10160..10771
                     /gene="cobC"
                     /locus_tag="ACHKBL_002604"
     CDS             10160..10771
                     /gene="cobC"
                     /locus_tag="ACHKBL_002604"
                     /EC_number="3.1.3.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043755 - alpha-ribazole phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin/alpha-ribazole phosphatase"
                     /protein_id="HFO5819457.1"
                     /translation="MRLWLIRHGETQANVDGLYSGHAPTPLTARGIEQAQNLHTLLHD
                     VSFDLVLCSELERAQHTARLVLSDRQHPVHIIPELNEMFFGDWEMRHHRDLMQEDAEN
                     YSAWCNDWQHAIPTNGEGFQAFSQRVERFIARLSEYQHYQNILVVSHQGVLSLLIARL
                     IGMPAESMWHFRVDQGCWSAIDINQKFATLRVLNSRAIGVENA"
     gene            11031..11348
                     /gene="rsfS"
                     /locus_tag="ACHKBL_002605"
     CDS             11031..11348
                     /gene="rsfS"
                     /locus_tag="ACHKBL_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HFO5819458.1"
                     /translation="MQGKALQDFVIDKIDDLKGQDIIALDVQGKSSITDCMIICTGTS
                     SRHVMSIADHVVQESRAAGLLPLGVEGENSADWIVVDLGDVIVHVMQEESRRLYELEK
                     LWS"
     gene            11352..11819
                     /gene="rlmH"
                     /locus_tag="ACHKBL_002606"
     CDS             11352..11819
                     /gene="rlmH"
                     /locus_tag="ACHKBL_002606"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HFO5819459.1"
                     /translation="MKLQLVAVGTKMPDWVQTGFTEYLRRFPKDMPFELIEIPAGKRG
                     KNADIKRILDKEGEQMLAAAGKNRIVTLDIPGKPWDTPQLAAELERWKLDGRDVSLLI
                     GGPEGLSPACKAAAEQSWSLSALTLPHPLVRVLVAESLYRAWSITTNHPYHRE"
     gene            11850..13751
                     /gene="mrdA"
                     /locus_tag="ACHKBL_002607"
     CDS             11850..13751
                     /gene="mrdA"
                     /locus_tag="ACHKBL_002607"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-transpeptidase MrdA"
                     /protein_id="HFO5819460.1"
                     /translation="MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQI
                     VRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKVDNVQQTL
                     DALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
                     YKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVL
                     HGQTGYEEVEVNNRGRVIRQLKEVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVT
                     DPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNDPNTPLVNRATQGVYPPASTVKP
                     YVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFF
                     YQVAYDMGIDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKPWYQGDTIP
                     VGIGQGYWTATPIQMSKALMILINDGIVKVPHLLMSTAEDGKQVPWVQPHEPPVGDIH
                     SGYWELAKDGMYGVANRPNGTAHKYFASAPYKIAAKSGTAQVFGLKANETYNAHKIAE
                     RLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAEN
                     PAVAAAEDH"
     gene            13754..14866
                     /gene="mrdB"
                     /locus_tag="ACHKBL_002608"
                     /gene_synonym="rodA"
     CDS             13754..14866
                     /gene="mrdB"
                     /locus_tag="ACHKBL_002608"
                     /gene_synonym="rodA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885876.1"
                     /note="rod shape-determining protein RodA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase MrdB"
                     /protein_id="HFO5819461.1"
                     /translation="MTDNPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIG
                     MMERKIGQIAMGLVIMVVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRW
                     LDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTAIALVLIFMPTLLVAAQPDL
                     GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPE
                     SDPLGAGYHIIQSKIAIGSGGLRGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVG
                     ILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGV
                     PLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV"
     gene            14877..15965
                     /gene="rlpA"
                     /locus_tag="ACHKBL_002609"
     CDS             14877..15965
                     /gene="rlpA"
                     /locus_tag="ACHKBL_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic peptidoglycan transglycosylase RlpA"
                     /protein_id="HFO5819462.1"
                     /translation="MRKQWLGICIAAGMLAACTSDDGQQQTVSVPQPAVCNGPIVEIS
                     GADPRFEPLNATANQDYQRDGKSYKIVQDPSRFSQAGLAAIYDAEPGSNLTASGEAFD
                     PTQLTAAHPTLPIPSYARITNLANGRMIVVRINDRGLYGNDRVISLSRAAADRLNTSN
                     NTKVRIDPIIVAQDGSLSGPGMACTTVAKQTYALPAPPDLSGGAGTSSVPVPQGDILP
                     VSNSTLKSEDPTGAPVTSSGFLGAPTTLAPGVLEGSEPTPAPQPVVTAPSTTPATSPA
                     MVTPQAASQSASGNFMVQVGAVSDQARAQQYQQQLGQKFGVPGRVTQNGAVWRIQLGP
                     FASKAEASTLQQRLQTEAQLQSFITTAQ"
     gene            16105..17316
                     /gene="dacA"
                     /locus_tag="ACHKBL_002610"
     CDS             16105..17316
                     /gene="dacA"
                     /locus_tag="ACHKBL_002610"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase DacA"
                     /protein_id="HFO5819463.1"
                     /translation="MNTIFSARIMKRLALTTALCTAFISAAHADDLNIKTMIPGVPQI
                     DAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGN
                     DAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVG
                     LMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFT
                     FNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKG
                     REAESKKLLTWGFRFFETVNPLKVGKEFASEPVWFGDSDRASLGVDKDVYLTIPRGRM
                     KDLKASYVLNSSELHAPLQKNQVVGTINFQLDGKTIEQRPLVVLQEIPEGNFFGKIID
                     YIKLMFHHWFG"
     gene            17426..17689
                     /gene="ybeD"
                     /locus_tag="ACHKBL_002611"
     CDS             17426..17689
                     /gene="ybeD"
                     /locus_tag="ACHKBL_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF493 family protein YbeD"
                     /protein_id="HFO5819464.1"
                     /translation="MKTKLNELLEFPTPFTYKVMGQALPELVDQVVEVVQRHAPGDYT
                     PTVKPSSKGNYHSVSITINATHIEQVETLYEELGKIDIVRMVL"
     gene            17790..18431
                     /gene="lipB"
                     /locus_tag="ACHKBL_002612"
     CDS             17790..18431
                     /gene="lipB"
                     /locus_tag="ACHKBL_002612"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HFO5819465.1"
                     /translation="MYQDKILVRQLGLQPYEPISQAMHEFTDTRDDSTLDEIWLVEHY
                     PVFTQGQAGKAEHILMPGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELV
                     TLLEQTVVNTLAELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
                     SPFLRINPCGYAGMEMAKISQWKPEATTNNIAPRLLENILALLNNPDFEYITA"
BASE COUNT         4539 a         4760 c         4834 g         4478 t
ORIGIN      
        1 gcgacgcaac cgggttgcac caccacaaat cagcggtagc accaaaaagg tgcgatttaa
       61 gaattttgct tcggaatggt gcggcgcatc tcgcaaaaac aaacaataaa acagcaatat
      121 ctttacattt agccaaacta aatgttacct aacaagcaca acactgcaca ataaagttgc
      181 agatgataac aacacaaaca ctcacaacgg gtatccatgc gttcttaacg cagaagataa
      241 aggagttgga tatgcaatta cgtaaacctg ccacagcaat cctggccctg gcgctttccg
      301 caggactggc acaggcagat gacgccgccc cggcagcagg cagtacgctg gacaaaatcg
      361 ccaaaaacgg cgtgattgtc gtcggtcacc gtgaatcttc agtacctttc tcttattacg
      421 acaatcagca aaaagtggtg ggctactcgc aggattactc caacgccatt gttgaagcag
      481 tgaaaaagaa actcaacaaa ccagacttac aggtgaaatt gattccgatc acctcacaaa
      541 accgtattcc actgctgcaa aacggcactt tcgattttga atgcggttct accaccaaca
      601 atgccgaacg ccaaaaacag gcggctttct ctgacactat tttcgtggtc ggtacgcgcc
      661 tgctgaccaa aaagggcggc gatatcaaag attttgccga cctgaaaggc aaagccgtag
      721 tcgtcacttc cggcaccacc tctgaagttt tgctcaacaa actgaatgaa gagcaaaaaa
      781 tgaatatgcg catcatcagc gccaaagatc acggtgactc tttccgtacc ctggaaagcg
      841 gtcgtgccgt tgcctttatg atggatgatg ctctgctggc cggtgaacgt gcgaaagcga
      901 agaaaccaga caactgggaa atcgtcggca agccgcagtc tcaggaagcc tatggttgca
      961 tgttgcgtaa agacgatccg cagttcaaaa agctgatgga tgataccatc gctcaggtgc
     1021 agacctccgg tgaagcggaa aaatggtttg ataagtggtt caaaaatcca attccgccga
     1081 aaaacctgaa catgaatttc gaactgtcag acgaaatgaa agcactgttc aaagaaccga
     1141 atgacaaggc actgaactaa ttacaagaac caggggcgga aattccagcc ctctcgattg
     1201 ttacgtagca cggacagact atacgcctgg tggtcgttcc ccatcgggcc tgaaaaccgc
     1261 aatacgctgg gtaacaatct tcgagggtag cagttaacgc tgctaccctc ttttttctgg
     1321 agtagattta tgtctataga ctggaactgg ggtatttttt tacaacaagc cccgttcggc
     1381 aacaccacct atcttggttg gatctggagt ggttttcagg tgacgatcgc tttgtcgatc
     1441 tgcgcctgga ttatcgcttt cctcgtcggt tcatttttcg gcattttacg taccgttcca
     1501 aaccgttttc tctccggtct gggtacgttg tatgtcgaac tgttccgcaa cgtgccgttg
     1561 attgtgcaat tctttacctg gtacctggtg atcccggagc tgctgccaga gaaaatcggc
     1621 atgtggttta aggcagagct ggatcccaat attcagtttt tcctttcatc catgctctgc
     1681 ctggggctgt ttaccgccgc ccgtgtttgc gaacaggtac gcgccgctat tcagtcgctg
     1741 ccgcgtgggc aaaaaaatgc cgcgctggcg atgggtctga cgctaccgca ggcttaccgc
     1801 tacgtactgc tgcctaatgc ttatcgcgtt atcgtcccgc cgatgacctc agagatgatg
     1861 aacctggtga aaaactccgc catcgcctcg actatcggtc tggtggatat ggcggcgcag
     1921 gcgggtaaat tgctcgatta ctcggcccac gcctgggagt cgtttaccgc catcacgctg
     1981 gcgtatgtat tgattaacgc ttttatcatg ctggtgatga cgctggttga acgtaaagtt
     2041 cgtctgcccg gcaacatggg gggcaaataa tgtacgagtt tgactggagt tccattgttc
     2101 cttccctgcc atatctgctc gacgggctgg tgatcacgct gaaaatcacc gtcacggcag
     2161 tggtgattgg tattttgtgg ggcaccatgc tggcggtgat gcgcttgtcc agctttgcgc
     2221 ctgttgcgtg gtttgccaaa gcctatgtta acgtattccg ctcgattcct ttagtcatgg
     2281 ttttgctgtg gttttacctg attgtgccgg gatttctgca aaacgtgctg ggattatcgc
     2341 caaaaaatga tatccgttta atttcggcaa tggtggcgtt ttcgatgttt gaagcggcct
     2401 actattcaga gattatccgt gccggtattc aaagtatttc ccgtggtcag tcgagcgccg
     2461 cgctggcgtt ggggatgact cactggcagt cgatgaaact gattattctg ccgcaggctt
     2521 tccgcgcgat ggtgccgctg ctgctcactc agggcatcgt actgttccag gatacctcgc
     2581 tggtgtatgt gttatctctg gccgatttct tccgtaccgc ttcaaccatt ggtgagcgtg
     2641 atggtacgca ggttgagatg atcctgtttg ccggatttgt ttatttcgtt attagcctta
     2701 gcgcgtcgtt gttggtcagc tacttgaaaa gaaggacagc ataatgatta ccctgaaaaa
     2761 tgtttcaaaa tggtatggtc actttcaggt gctgaccgac tgctcaaccg aagtgaaaaa
     2821 aggcgaagtg gtggtggttt gcggcccgtc tggttccggc aaatcaacgc tgattaaaac
     2881 cgtcaacggc ctcgaaccgg tgcagcaagg tgaaatcacc gtcgatggca tcgtggttaa
     2941 cgacaagaaa accgatctgg caaagctgcg ttcccgcgtc gggatggtat tccagcattt
     3001 cgagctgttc cctcatctgt cgattatcga aaacctgacc ctggcgcagg tgaaagtgct
     3061 taaacgcgat aaagcgccgg ctcgtgaaaa agcattgaaa ttgctggagc gcgtcgggct
     3121 ttccgcacat gccaataagt ttccggcgca gctttccggc ggtcagcagc agcgtgtggc
     3181 gatcgctcgc gcattgtgta tggatcctat tgccatgctg ttcgacgaac cgacatcggc
     3241 gctggatccg gagatgatca acgaagtgct ggacgtgatg gtggaactgg cgaacgaagg
     3301 aatgaccatg atggtggtga cccacgaaat gggctttgcc cgtaaagtgg cgaatcgggt
     3361 gatctttatg gacgagggta aaattgtcga agactcgccg aaagacgctt tcttcgatga
     3421 tccgaaatcg gaccgcgcaa aagacttcct cgcgaaaatc ctgcattaat cactctggcg
     3481 cgcataccca agtgcgcgcc atctcacgct ttatcgtttc gctcctcgta tctgttacat
     3541 cctagcggta cccttgtcgc aaagaagcac acaacaagga gcaacaatgg cactgccaat
     3601 tctgttagat tgcgacccag gtcatgacga cgctatcgca atagttcttg ccctcgcctc
     3661 accagagctt gatgtcaaag caattacgtc ttccgccgga aaccagacac cagaaaaaac
     3721 cttacgcaat gttctgcgta tgctgacctt gcttaatcgc accgatattc cggtagcagg
     3781 cggcgcggta aaaccgttaa tgcgtaattt gattattgcg gacaatgtgc acggcgaaag
     3841 cggtctcgac ggcccggcat taccggaacc gacgttcgca ccgcaaaact gtacggcggt
     3901 ggagctaatg gcgaaaacgc tgtgtgaaag tgaggaacct atcaccattg tttcaaccgg
     3961 accgcaaact aacgttgcct tgctgctcaa tagccacccg gaactgcata gcaaaattgc
     4021 ccgtatcgtg attatgggcg gcgcaatggg gcttggtaac tggacgcctg cggctgaatt
     4081 taatatttac gttgacccgg aagcggcaga aattgtcttc cagtcaggaa ttccggtggt
     4141 gatggccggt ctggatgtta ctcacaaagc acaaatccac gttgaagaca ctgagcgttt
     4201 ccgcgcgatt ggcaaccctg tttcaaccat tgttgccgaa ctgctggatt tcttcctcga
     4261 atatcataaa gacgaaaaat ggggctttgt cggcgcacca ctgcatgacc catgcaccat
     4321 cgcctggctg ttgaaaccgg agttgtttac cactgttgag cgctgggttg gcgtggaaac
     4381 acaagggaaa tatacccagg gtatgacggt tgttgattat tattatctga ctggcaataa
     4441 accgaatgcc accgtaatgg tcgatgttga tcgtcagggc tttgttgatt tactggctga
     4501 tcgtctgaaa ttttacgctt aatcactcgc cggatgacat cacgccatcc ggcaccactc
     4561 atttcattac ggctcgaacg gacgtctctg gaactgatca tgaccacatt tcgggcatag
     4621 cgtcagcact tccggtgtat agatcgggag atggaagtga catttctcgc agaccagatt
     4681 tcccagccct accacttctc cgctgtgata aaccccgtga tgattgaggt cctggaaaac
     4741 ttcgcgccat tcaagctgcg ttttatcggt gatgtctgcc agctcctgcc acaagctttc
     4801 tttaatcacc cgcataaaga cgctgtcaga ttcttctttc aggctctctt catagctcat
     4861 ggcgaactct tccaggtcac gtctgacagc tcgcgtcagc tcatcgacct cggttcgcgt
     4921 taactcccct gtttttatta cgcgctcgcg cgcctgttcc accagtgcgt cgatatcacg
     4981 ttcgccattg cgcaggcgtt cgctcagtga cgcgaccagt tcacggtaat attgagcaac
     5041 cttgttcatc gtttcatctc ccgggtcgtt aactgactat aataatagac gttctttgtt
     5101 ccttgctttg ctaatacgcc cacaaactct gtaaattaat gtatgaataa tccgggcatc
     5161 atttcaacgt cgagtgcgcg aaaggctgtt ttgacgcgga cgtttgggct atgctatgcg
     5221 gatctgaaaa accacctcaa cgctacattt gtagccgtat tgaaaacagg accactggct
     5281 gccatgcaag agcaataccg cccggaagag atagaatcca aagtacagct tcattgggat
     5341 gagaagcgca catttgaagt aaccgaagac gagagcaaag agaagtatta ctgcctgtct
     5401 atgcttccct atccttctgg tcgactacac atgggccacg tacgtaacta caccatcggt
     5461 gacgtgatcg cccgctacca gcgtatgctg ggcaaaaacg tcctgcagcc gatcggctgg
     5521 gacgcgtttg gtctgcctgc ggaaggcgcg gcggtgaaaa acaacaccgc accggcaccg
     5581 tggacgtacg acaacatcgc gtacatgaaa aaccagctca aaatgctggg ctttggttat
     5641 gactggagcc gcgagctggc aacctgtacg ccggaatact accgttggga acagaaattc
     5701 ttcaccgagc tgtataaaaa aggcctggta tataagaaga cttctgcagt caactggtgc
     5761 ccgaacgacc agaccgtact ggcgaacgaa caagttatcg acggctgctg ctggcgctgc
     5821 gataccaaag ttgagcgtaa agagatcccg caatggttta tcaaaatcac cgcttacgct
     5881 gacgagctgc tcaacgatct ggataaactg gatcactggc cagacaccgt taaaaccatg
     5941 cagcgtaact ggatcggtcg ttccgaaggc gtggaaatca ccttcaacgt taacgactat
     6001 gacaacacgc tgaccgttta cactacccgc ccggacacct ttatgggttg tacctacctg
     6061 gcggtggctg cgggtcatcc gctggcacag aaagcggcgg aaaataatcc tgaactggcc
     6121 gcctttattg acgaatgccg taacaccaaa gttgccgaag ctgaaatggc gacgatggag
     6181 aaaaaaggcg tcgatactgg ctttaaagcg gttcacccat taacgggcga agaaattccc
     6241 gtttgggcag caaacttcgt attgatggaa tacggcacgg gcgcagttat ggcggttccg
     6301 ggtcacgacc agcgcgacta cgagtttgcc tctaaatacg gcctgaacat caaaccggtt
     6361 atcctggcag ctgacggctc tgagccagat ctttctcagc aagccctgac tgaaaaaggc
     6421 gtgctgttca actctggcga gttcaacggt cttgaccatg aagcggcctt caacgccatc
     6481 gccgataaac tgactgcgat gggcgttggc gagcgtaaag tgaactaccg cctgcgcgac
     6541 tggggtgttt ctcgtcagcg ttactggggc gcgccgattc cgatggtgac gctggaagac
     6601 ggtaccgtaa tgccgacccc ggacgaccag ctgccggtga tcctgccgga agatgtggtg
     6661 atggacggca ttaccagccc gattaaagca gatccggagt gggcaaaaac taccgttaac
     6721 ggtatgccag cgctgcgtga aaccgacact ttcgacacct ttatggagtc ctcctggtac
     6781 tatgcgcgct acacttgccc ggagtacaaa gaaggtatgc tggattccaa agcggctaac
     6841 tactggctgc cggtggatat ctacattggt ggtatcgaac acgccattat gcacctgctc
     6901 tacttccgct tcttccacaa actgatgcgt gatgcaggca tggtgaactc tgacgaacca
     6961 gcaaaacagt tgctgtgtca gggtatggtg ctggcagatg ccttctacta tgttggcgaa
     7021 aatggcgaac gtaactgggt ttccccggtt gatgctatcg ttgaacgtga cgagaaaggc
     7081 cgtatcgtga aagcgaaaga tgcggcaggc catgaactgg tttataccgg catgagcaaa
     7141 atgtccaagt cgaagaacaa cggtatcgac ccgcaggtga tggttgaacg ttacggcgcg
     7201 gacaccgttc gtctgtttat gatgtttgct tctccggctg atatgactct cgaatggcag
     7261 gaatccggcg tggaaggggc taaccgcttc ctgaaacgtg tctggaaact ggtttacgag
     7321 cacacagcga aaggtgatgt tgcggcactg aacgttgatg cgctgactga agatcagaaa
     7381 gcgctgcgtc gcgatgtgca taaaacgatc gctaaagtga ccgatgatat cggccgtcgt
     7441 cagaccttca acaccgcaat tgcggcgatt atggagctga tgaacaaact ggcgaaagca
     7501 ccgaccgatg gcgagcagga ccgcgccctg atgcaggaag cactgctggc cgttgtccgt
     7561 atgcttaacc cgttcacccc gcacatctgc ttcacgctgt ggcaggaact gaaaggcgaa
     7621 ggcgatatcg acaacgcgcc gtggccggtt gctgacgaaa aagcgatggt ggaagactcc
     7681 acgctggtcg tggtgcaggt taacggtaaa gtccgtgcaa aaatcaccgt tccggtggac
     7741 gcaacggaag aacaggttcg cgaacgtgct ggccaggaac atctggtagc aaaatatctt
     7801 gatggcgtta ccgtgcgtaa agtgatttac gtacccggta aactcctcaa tctggtcgtt
     7861 ggctaagcgc gggaggaagc gtgcgatatc tggcaacatt gttgttatct ctggcggtgt
     7921 taatcaccgc cgggtgtggc tggcatctgc gtgataccac gcaggttcct tccactatga
     7981 aggtcatgat cctggactca ggcgatccga acgggccatt aagccgtgcg gtgcgtaacc
     8041 agttacgtct aaatggtgtc gagttgcttg ataaagaaac cacgcgtaag gacgttccat
     8101 ccctgcgttt gggtaaagtg agcatcgcga aagataccgc atcggtattc cgtaacggtc
     8161 aaacagcaga gtatcagatg atcatgacgg tcaatgcgac cgtgttgatc cccggccgtg
     8221 acatctaccc gattagcgcc aaagtcttcc gttcgttctt cgataacccg caaatggcgt
     8281 tagcgaaaga taacgaacaa gacatgatcg taaaagagat gtacgaccgt gctgccgaac
     8341 agctgattcg taagctgcca agcatccgtg ctgcggatat tcgttccgac gaagaacaga
     8401 cgtcgaccac aacggatact ccggcaacgc ctgcacgcgt ctctaccacg ctgggtaact
     8461 gatgattcgg ttgtacccgg aacaactccg cgcgcagctc aatgaagggc tgcgcgcggc
     8521 gtatctttta cttggtaacg atcctctgtt attgcaggaa agccaggacg ctattcgtca
     8581 ggtagctgcg gtacaaggat tcgaagaaca ccacactttt tccattgatc ccaacactga
     8641 ctggaatgcg atcttttcgt tatgccaggc catgagtctg tttgccagtc gacaaacgct
     8701 attgctgttg ttaccagaaa acggaccgaa tgcggcgatc aatgaacaac ttctcacact
     8761 caccggactt ctgcatgacg acctgctgtt gatcgtccgc ggcaataaat taagcaaagc
     8821 gcaagaaaat gccgcctggt ttactgctct tgcgaatcgc agcgtgcagg tgacctgtca
     8881 gacaccggag caggctcagc ttccccgctg ggttgctgtg cgcgcaaaac agctcaactt
     8941 agagctggat gacgcggcga atcaggtgct ctgctactgt tatgaaggta acctgctggc
     9001 gctggctcag gcactggagc gtttatcgct actctggcca gacggcaaac tgacattacc
     9061 tcgcgttgaa caggcggtga atgatgccgc gcatttcacc ccttttcatt gggttgatgc
     9121 tttgttgatg ggaaaaagta agcgcgcgtt gcatattctt cagcaactgc gtctggaagg
     9181 cagcgaaccg gttattttgt tgcgcacatt acaacgtgaa ctgttgttac tggttaacct
     9241 gaaacgccag tctgcccata tgccactgcg tgcgttgttt gataagcatc gggtatggca
     9301 gaaccgccgg ggcatgatgg gcgaggcgtt aaatcgctta agccatccgc agttacgtca
     9361 ggccgtgcaa ctcctgacac gaacggaact caccctcaaa caagattacg gtcagtcagt
     9421 gtgggcagag cttgaagggt tatctcttct gttgtgccat aaacccctgg cggacgtatt
     9481 tatcgacggt tgatatgaaa tctttacagg ctctgtttgg cggcaccttt gatccggtgc
     9541 actatggtca tcttaaaccc gtggaaacgc tggcgaattt gattggtctg acgcgggtca
     9601 caatcatccc taataatgtt cctccgcatc gtccccagcc ggaagcaaac agcatgcagc
     9661 gtaaacacat gcttgaactg gcgattgccg acaagccatt atttactctt gatgaacgcg
     9721 agctaaagcg caatgccccc tcttacactg cgcaaacact gaaagagtgg cggcaggagc
     9781 aaggaccgga cgtgccgctg gcgtttatta ttggtcagga ttcattgctg acctttccga
     9841 cctggtacga atacgaaacg atactcgaca atgcacattt gatcgtctgt cggcgtcctg
     9901 gttacccact tgaaatggca caaccacaat atcagcaatg gctggaggat catttgacac
     9961 ataacccgga agatcttcac cttcagcctg ccggtaaaat ttatctggct gaaacgccgt
    10021 ggtttaacat ctcggcgacc atcatccgcg aacgtttgca aaatggtgaa tcgtgtgagg
    10081 atttattacc ggaaccggta ctgacttaca ttaaccaaca aggcttgtat cgctgatacc
    10141 tgtcgttacg gagggcatga tgcgactgtg gttaattcgt catggtgaaa cgcaagcgaa
    10201 tgtcgatggt ctgtacagcg gtcatgcgcc cacacccctg accgcgcgcg gtattgagca
    10261 agcgcaaaat ctgcatacgc tgctacatga tgtttccttt gacctggttt tatgcagtga
    10321 actggaacgg gcacagcata ccgcgcgact ggttcttagt gaccgccagc accccgttca
    10381 tatcatccct gaactcaatg aaatgttttt tggcgactgg gagatgcgac atcatcgtga
    10441 cctcatgcaa gaagatgccg aaaactatag cgcgtggtgc aatgactggc agcatgctat
    10501 ccccacgaac ggtgaaggat ttcaggcatt ttcgcaacgt gtggaacgat ttatcgcaag
    10561 gcttagtgaa tatcagcact atcagaatat tttagtcgtc agccatcagg gcgtactgag
    10621 tctgttaatt gctcgtttaa ttggcatgcc tgccgagtct atgtggcatt tccgcgttga
    10681 ccaggggtgt tggagcgcca ttgatatcaa ccaaaaattc gcgacgctac gcgtcctcaa
    10741 tagccgtgcc atcggggtcg agaatgcatg acttttctgt ttttttacgg gtaagccgca
    10801 acgaccattg acagccccgg gcaggctgat attctccgca gccagacttt ttccgccaga
    10861 cacgactttg tagaaattgt tttacaaaaa tggcgatgca atctgcggcg cggggtggga
    10921 tgatagccca ctttcgaaag ccgattcggc gacaattgtc ccgaaatcgc ctctggttca
    10981 ggtatactga cagaccattt ttatctattt gattcaccca gggggaaaac ttgcagggta
    11041 aagcactcca ggattttgtt atcgacaaaa ttgatgacct caaaggtcag gacatcatcg
    11101 ccttagacgt tcagggcaaa tccagcatca ccgactgcat gatcatctgt acgggtacgt
    11161 ccagccgcca tgttatgtcc attgctgatc acgttgtgca ggagtctcgc gcagcgggcc
    11221 tgttaccgct cggcgtagaa ggtgaaaaca gcgccgactg gattgtcgtg gatttgggcg
    11281 atgtgattgt ccatgtcatg caggaagaga gccgtcgcct gtatgaactg gaaaaactct
    11341 ggagttaatg cgtgaagctg caacttgtcg ccgtgggaac gaaaatgccg gactgggtac
    11401 aaaccggttt taccgagtac ctgcgtcgtt ttccgaaaga tatgcccttc gagctgattg
    11461 aaattccggc cggaaaacgc ggcaagaatg cggacatcaa gcgcatactc gacaaagagg
    11521 gtgagcagat gttggcggcc gcaggcaaaa accgcattgt caccctcgat attccaggca
    11581 agccctggga tacgccgcag ttagccgctg agctggaacg ctggaagctg gatggtcgcg
    11641 acgtcagtct actgattggc gggcctgaag ggttgtcgcc tgcctgtaaa gcggcggcgg
    11701 agcagagctg gtcgctgtcg gcacttaccc ttccccatcc gctggttcgc gtgctggtcg
    11761 cagagagtct gtaccgggcg tggagcatca ccaccaacca tccttatcac cgtgagtgat
    11821 aagtgagctt tgagtagaaa acgcagcgga tgaaactaca gaactctttt cgcgactata
    11881 cggcagagtc cgcgctgttt gtgcgccggg cgctggtcgc ctttttgggg attttgctgc
    11941 tgaccggcgt gcttatcgcc aacctgtata atctgcaaat tgttcgcttt accgactacc
    12001 agacccgctc taatgaaaac cgcattaagc tggtgcctat cgcacccagc cgcggcatta
    12061 tctacgaccg taacggtatc cctctggctc tcaaccgcac tatctaccag atagaaatga
    12121 tgccggagaa agtcgataac gtgcagcaaa cgctggacgc tttgcgcagc gtggttgatc
    12181 tgaccgatga tgatattgcg gcattccgaa aagagcgcgc acgttcacac cgtttcacct
    12241 ctattccggt gaaaactaac ctgaccgaag tacaagtagc tcgctttgcc gtcaaccagt
    12301 accgttttcc gggtgttgaa gttaaaggct ataaacgtcg ttactatcct tacggttcgg
    12361 cgttgaccca cgtcatcggc tatgtgtcga aaatcaacga taaagacgtc gaacgcctga
    12421 ataatgacgg caaactggcc aactatgcgg caacgcatga tatcggtaag ctgggcattg
    12481 aacgttacta tgaagacgtg ctgcacggtc agaccggtta tgaagaggtt gaagttaaca
    12541 accgtgggcg tgttatccgc cagttaaaag aagtaccacc gcaagccgga cacgatattt
    12601 acctgacgct ggatctcaaa ctccagcaat atattgaaac gctgctggcg ggtagccgcg
    12661 cagctgtggt agtcaccgat ccgcgtacag gtggggtgtt ggcgctggtt tccacgccta
    12721 gttatgaccc aaacctgttt gttgacggta tctccagcaa agattattcc gccttgctga
    12781 acgacccgaa tacaccgctg gtgaaccgcg ccacacaggg ggtttatcct cccgcgtcta
    12841 cagttaaacc ctatgttgcg gtttcggcat tgagtgccgg ggtgatcacg cgcaatacga
    12901 cgctgtttga ccccggctgg tggcaactgc cagggtcgga aaaacgttat cgtgactgga
    12961 aaaaatgggg ccacgggcgt ctgaatgtca caagatcgct ggaagaatct gcggatacct
    13021 tcttctatca ggtggcctac gatatgggga tcgatcgcct ctccgaatgg atgggtaaat
    13081 tcggttatgg tcattacacc ggtatcgacc tggcggaaga acgttccggc aacatgccta
    13141 cccgcgaatg gaaacagaaa cgctttaaaa aaccgtggta tcagggtgac accattccgg
    13201 ttggtatcgg tcagggttac tggacagcga ccccaatcca gatgagtaag gcactgatga
    13261 tcctgattaa tgacggtatc gtgaaggttc cgcatttgct gatgagcacc gccgaagacg
    13321 gcaaacaggt gccatgggta caaccgcatg aaccgcccgt cggcgatatt cattccggtt
    13381 actgggagct ggcgaaagac ggtatgtacg gtgttgctaa ccgccctaac ggtacggcgc
    13441 ataaatattt tgccagcgca ccgtacaaaa ttgcggcgaa atccggtacc gctcaggtct
    13501 tcggtctgaa agcgaacgaa acctataatg cgcacaaaat tgccgagcgt ttacgtgacc
    13561 acaaactgat gaccgccttt gcgccataca acaatccgca agtggctgtc gccatgattc
    13621 tggagaacgg tggcgcgggt ccggcggttg gtacgctgat gcgccagatc ctcgaccaca
    13681 ttatgctggg tgataacaac accgatctgc ctgcggaaaa tccagcggtt gccgcagcgg
    13741 aggaccatta atcatgacag ataatccgaa taaaaaaaca ttctgggata aagtccatct
    13801 cgatcccaca atgctgctga tcttactggc attgctggtt tacagcgccc tggttatctg
    13861 gagcgccagc ggtcaggata ttggcatgat ggagcgtaaa atcggccaga ttgcgatggg
    13921 tctggtcatc atggtggtga tggcgcaaat tcctccacgc gtttatgaag gctgggcccc
    13981 ctatctctat atcatctgta ttattttgct ggtggcggta gatgctttcg gtgccatctc
    14041 taaaggcgct caacgctggc tggacctcgg tattgttcgt tttcaaccgt cggaaattgc
    14101 caagatagcc gtaccgttga tggttgcacg ctttatcaac cgcgacgttt gcccaccttc
    14161 attgaagaat acagctatcg cgctggtgct gatatttatg cccacgctgc tggtggctgc
    14221 acagcctgac ttgggaacat caatcctcgt tgcgctttcc ggcctgtttg tactgttcct
    14281 ctctggcctt agctggcgtc tgattggcgt cgcagtagtg ctggtagcgg cgttcattcc
    14341 gattctgtgg ttctttctga tgcatgatta ccagcgccag cgcgtaatga tgctcctgga
    14401 cccggaatca gacccactcg gcgcaggcta tcacattatt cagtctaaaa ttgctattgg
    14461 ctccggcgga ttacgcggca aaggctggct gcacggcact cagtcacagc ttgaatttct
    14521 ccccgaacgc cataccgact ttatcttcgc ggtactggcg gaagagctgg gattagtggg
    14581 cattttgatt ctgctcgctc tctacattct gctgatcatg cgcgggctgt ggatagccgc
    14641 cagagcgcaa accacctttg gtcgcgtcat ggctggcggc ttaatgctga tattattcgt
    14701 ttatgtcttc gtaaatattg gtatggtaag cggtattctg ccggttgtag gggttccgct
    14761 cccactggtc agttatggag gatcggcgct aattgtgctg atggctgggt tcgggattgt
    14821 aatgtcaatc cacacccaca ggaaaatgtt gtcgaaaagc gtgtaagagg tgcgcaatgc
    14881 gtaagcagtg gctcgggatc tgcatcgcgg caggaatgct cgcggcatgt acaagcgatg
    14941 atggtcagca acagacggta agtgtaccgc agcctgcggt atgtaacggc cccatagttg
    15001 aaattagcgg ggcagacccg cgtttcgaac cactgaacgc gacggcaaat caggattacc
    15061 agcgcgacgg gaaaagctac aaaatcgtgc aggatccgtc tcgatttagc caggcgggac
    15121 tggcggcaat ctatgatgcc gaacccggca gtaacctgac ggcctctggc gaagctttcg
    15181 atccgacaca gttgacggcg gcccatccaa cgcttccaat ccccagctac gccagaatca
    15241 ctaacctggc taacgggcga atgatcgtgg tgcgcattaa tgatcgcggc ctttatggca
    15301 acgaccgcgt gatttcgctt tctcgcgcgg cagctgaccg tcttaacacg tcaaacaaca
    15361 ccaaagttcg tatcgatccg attattgtcg cccaggatgg ttcgctttct ggtcctggta
    15421 tggcttgtac cacagtcgcc aaacagactt acgccctgcc agcacctccc gatttaagcg
    15481 gtggcgcggg aacaagttca gtgcctgtcc cgcagggtga cattcttccg gtcagtaatt
    15541 cgacgctaaa aagcgaagat ccgaccggcg cgccggtaac cagcagcggt ttcctcggcg
    15601 caccaacgac cttagcgcct ggtgtactgg aaggcagcga accgacgcct gcgccacagc
    15661 ccgttgttac agctccgtcg acaacgcctg caacctcacc tgcaatggtg acaccgcaag
    15721 ccgcctcgca aagcgccagc ggcaacttta tggtgcaagt cggggccgtt agcgatcagg
    15781 ctcgtgcgca acagtaccaa cagcaactgg gacagaagtt cggcgtcccc ggtcgcgtaa
    15841 ctcaaaatgg cgcggtctgg cggatccagc ttggcccatt cgccagcaaa gccgaagcca
    15901 gtaccttgca gcaacgtttg caaaccgaag cccaattaca gtcatttatt accaccgcgc
    15961 agtagcgtaa agcaggcatc tgatgtgtca atttctgtaa atgacgttaa caaagtcacg
    16021 cggaaagtca gatgcctgcc ggtagtgcat ttgctatagt agggcacttt ttttaattcc
    16081 atcacggatg tcgtagttca gaccatgaat accatttttt ccgctcgtat catgaagcgc
    16141 ctggcgctca ccacggctct ttgcacagcc tttatctctg ctgcacatgc cgatgacctg
    16201 aatatcaaaa ccatgatccc gggtgtaccg cagatcgacg cggagtccta catcctgatt
    16261 gactataact ccggcaaagt actcgccgaa cagaacgctg atgtccgccg cgatcctgcc
    16321 agcctgacca aaatgatgac cagttacgtt atcggccagg caatgaaagc cggtaaattt
    16381 aaagaaactg atttagtcac tatcggcaac gacgcatggg ccaccggtaa cccggtgttt
    16441 aaaggttctt cgctgatgtt cctcaaaccg ggcatgcagg ttccggtttc tcagctgatc
    16501 cgcggtatta acctgcaatc gggtaacgat gcttgtgtcg ccatggccga ttttgccgct
    16561 ggtagccagg acgcttttgt tggcttgatg aacagctacg ttaacgccct gggcctgaaa
    16621 aatacccact tccagacggt acacggtctg gatgctgatg gtcagtacag ctccgcgcga
    16681 gatatggcgc tgatcggcca ggcgttgatc cgtgacgtac cgaatgaata ctcgatctat
    16741 aaagaaaaag aatttacgtt taacggtatt cgccagctga accgtaacgg cctgttatgg
    16801 gataacagcc tgaatgtcga cggcatcaaa accggacaca ctgacaaagc aggttacaac
    16861 cttgttgctt ctgcgactga aggccagatg cgcttgatct ctgcggtgat gggcggacgt
    16921 acttttaaag gccgtgaagc cgaaagtaaa aaattgctaa cctggggctt ccgtttcttc
    16981 gaaaccgtta atcctctgaa agtaggtaaa gagttcgcct ctgaaccggt ttggtttggt
    17041 gattctgatc gcgcttcgtt aggggttgat aaagacgtgt acctgaccat tccgcgtggt
    17101 cgcatgaaag atctgaaagc cagctatgtg ctgaacagca gtgaattgca tgcgccgctg
    17161 caaaagaatc aggtcgtcgg aactatcaac ttccagcttg atggcaaaac gatcgaacaa
    17221 cgcccgctgg ttgtgctgca agaaatcccg gaaggtaact tcttcggcaa aatcattgat
    17281 tacattaaat taatgttcca tcactggttt ggttaaaaat tgaacacttg aaagtgtaat
    17341 ttccgtcccc atatactaag catcagtaaa aaactcccgc cttctggcgg gagttgctat
    17401 ttaattacgt tacgccggag ctgacatgaa aaccaaactt aacgaactgc ttgaattccc
    17461 tactcctttt acttacaaag ttatggggca ggcgttacct gagctggttg atcaggtggt
    17521 tgaagtggta cagcgccatg cgccaggtga ctacacccca acggtaaaac caagcagcaa
    17581 aggcaactac cactcggtat ctatcactat caacgccact catatcgagc aggttgaaac
    17641 actgtatgaa gaactgggca aaatcgatat tgtccgcatg gttctgtaac tcgcttctcc
    17701 gttacccggc tcctggtcgg gtaactcccc tctcctcgct gtgatatact tttcccccac
    17761 ttttactcat tctccacgga gatgccgttt tgtatcagga taaaattctt gtccgccagc
    17821 tcggtcttca gccttacgag ccaatctccc aggctatgca tgaattcacc gatacccgcg
    17881 atgatagtac ccttgatgaa atctggctgg tcgagcacta tccggtattc acccaagggc
    17941 aggcaggaaa ggcggagcac attttaatgc cgggtgatat tccggtgatc cagagcgatc
    18001 gcggtgggca ggtgacttat cacgggccgg ggcaacaggt gatgtatgtg ttgcttaacc
    18061 tgaaacgccg taaactcggt gtgcgtgaac tggtgacctt gcttgagcaa acagtggtga
    18121 ataccctggc tgaactgggt atagaagcgc atcctcgggc tgacgcgcct ggtgtctatg
    18181 tcggggaaaa gaaaatttgc tcactgggtt tacgtattcg acgcggttgt tcattccacg
    18241 gtctggcatt aaacgtcaat atggatcttt caccattttt acgtattaat ccttgtgggt
    18301 atgcaggaat ggaaatggct aaaatatcac aatggaaacc cgaagcgacg actaataata
    18361 ttgcaccacg tttactggaa aatattttag cgctactaaa caatcctgac ttcgaatata
    18421 ttaccgctta attccataca tcaatggccc aatttacact gggtcattac tcataatacc
    18481 tgctaatgct ttacatctcc ttcgcgtttg tatattttct ttatcacttg cagaaatgcg
    18541 atgctacgcc ttgctccgta gcccacctaa ttgattaacc cattgaaata attgaccatg
    18601 gtgagcaaaa g
//
LOCUS       DAZFLD010000090        18559 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.090, whole genome shotgun sequence.
ACCESSION   DAZFLD010000090 DAZFLD010000000
VERSION     DAZFLD010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18559
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.090"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            3..260
                     /locus_tag="ACHKBL_002613"
     CDS             3..260
                     /locus_tag="ACHKBL_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001111998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1889 family protein"
                     /protein_id="HFO5819466.1"
                     /translation="MDVSAPTPSSMNESTAKGIFKYLKELGVPASAADITARADQEGW
                     NPGFTEKMVGWAKKMESGERSVIKNPEYFSTYMQEELKALV"
     gene            complement(380..559)
                     /gene="yoaH"
                     /locus_tag="ACHKBL_002614"
     CDS             complement(380..559)
                     /gene="yoaH"
                     /locus_tag="ACHKBL_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HFO5819467.1"
                     /translation="MFAGLPSLTHEQQQKAVERIQELMAQGMSSGQAIALVAEELRAN
                     HAGERIVARFEDEDE"
     gene            633..1994
                     /gene="pabB"
                     /locus_tag="ACHKBL_002615"
     CDS             633..1994
                     /gene="pabB"
                     /locus_tag="ACHKBL_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 1"
                     /protein_id="HFO5819468.1"
                     /translation="MKTLSPAVITLPWRQDAAEFYFSRLSHLPWAMLLHSGYADHPYS
                     RFDIVVAEPICTLTTFGKETVVSENEKRTTTTDDPLQVLQQVLDRADIRPAHNEDLPF
                     QGGALGLFGYDLGRRFESLPEIAQQDIVLPDMAVGIYDWALVVDHQRQTVSLLSHNDV
                     NARRAWLESQQFSPQEGFTLTSDWQSNMTREQYGEKFRQVQEYLHSGDCYQVNLAQRF
                     HATYSGDEWQAFLQLNQANRAPFSAFLRLEQGAILSLSPERFILCDNSEIQTRPIKGT
                     LPRLPDPQEDSKQAEKLANSAKDRAENLMIVDLMRNDIGRVAVAGSVKVPELFVVEPF
                     PAVHHLVSTITARLPEQLHTSDLLRAAFPGGSITGAPKVRAMEIIDELEPHRRNAWCG
                     SIGYLSFCGNMDTSITIRTLTAINGQIYCSAGGGIVADSQEEAEYQETFDKVNKILRQ
                     LEK"
     gene            1997..2575
                     /gene="nudL"
                     /locus_tag="ACHKBL_002616"
     CDS             1997..2575
                     /gene="nudL"
                     /locus_tag="ACHKBL_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HFO5819469.1"
                     /translation="MEHRSLTLDDFLSRFQLLRPQMNRETLNHRQAAVLIPIVRRPQP
                     GLLLTQRSIHLRKHAGQVAFPGGAVDDTDASVIAAALREAEEEVAIPPSAVEVIGVLP
                     PVDSVTGYQVTPVVGIIPPDLPYRASEDEVSAVFEMPLAQALHLGRYHPLDIYRRGDS
                     HRVWLSWYEQYFVWGMTAGIIRELALQIGVKP"
     gene            2759..4123
                     /gene="sdaA"
                     /locus_tag="ACHKBL_002617"
     CDS             2759..4123
                     /gene="sdaA"
                     /locus_tag="ACHKBL_002617"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HFO5819470.1"
                     /translation="MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTR
                     VAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDIDSIPGFIRDVEERERLLLAQGR
                     HEVDFPRDNGMRFHNGNLPLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQD
                     AANEVSVPYPFKSATELLAYCNETGYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQA
                     CIDRGMNTEGVLPGPLRVPRRASALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENA
                     AGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPC
                     IERNAIASVKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIK
                     VQCD"
     gene            4254..5852
                     /gene="pdeD"
                     /locus_tag="ACHKBL_002618"
     CDS             4254..5852
                     /gene="pdeD"
                     /locus_tag="ACHKBL_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFO5819471.1"
                     /translation="MQKAQRIIKTYRRNRMIVCTICALVTLASTLSVRFISQRNLNQQ
                     RVVQFANHAVEELDKVLLPLQAGSEVLLPLIGLPCSVAHLPLRKQAAKLQTVRSIGLV
                     QDGTLYCSSIFGYRNVPVVDILAELPAPQPLLRLTTDHALIKGSPVLIQWTPAAGSSK
                     AGVMEMINIDLLAAMLLEPQLPQISSASLTVDDRHLLYGNGLVDSLPQPENNENYQVS
                     SQRFPFTINVNGPGATALAWHYLPTQLPLAVLLSLLVGYIAWLATAYRMSFSREINLG
                     LAQHEFELFCQPLLNARSQQCIGVEILLRWNNPRQGWISPDVFIPIAEEHHLIVPLTR
                     YVMAETIRQRHVFPMSSQFHVGINVAPSHFRRGVLIKDLNQYWFSAHPIQQLILEITE
                     RDALLDVDYRIARELHRKNVKLAIDDFGTGNSSFSWLETLRPDVLKIDKSFTAAIGSD
                     AVNSTVTDIIIALGQRLNIELVAEGVETQEQAKYLLRHGVHILQGYLYAQPMPLRDFP
                     KWLAGSQPPPARHNGHITPIMPLR"
     gene            complement(5856..7412)
                     /gene="yoaE"
                     /locus_tag="ACHKBL_002619"
     CDS             complement(5856..7412)
                     /gene="yoaE"
                     /locus_tag="ACHKBL_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family cation transport protein YoaE"
                     /protein_id="HFO5819472.1"
                     /translation="MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRD
                     KARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMDFSFSGRDLIMLFGGIFLLFKAT
                     TELHERLENRDHDSGHGKGYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
                     VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAI
                     GFSIIIEVFNQIARRNFIRHQSTLPLRARTADAILRLMGGKRQANVQHDADNPMPMPI
                     PEGAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVDEIREQLLSSPHSLF
                     PVCRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRRAR
                     GSFVIVTNEFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGDGWLVKGGTDLHALQ
                     QALDVEHLADDDDIATVAGLVISANGHIPRVGDVIDVGPLHITIIEANDYRVDLVRIV
                     KEQPAHDEDE"
     gene            complement(7400..7558)
                     /gene="yoaL"
                     /locus_tag="ACHKBL_002620"
     CDS             complement(7400..7558)
                     /gene="yoaL"
                     /locus_tag="ACHKBL_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaL"
                     /protein_id="HFO5819473.1"
                     /translation="MDRHRRHFSIRPFNACLSGTLCRTFRLHFVVTPALFLASNSYSL
                     SRSLSWNS"
     gene            7875..8846
                     /gene="manX"
                     /locus_tag="ACHKBL_002621"
     CDS             7875..8846
                     /gene="manX"
                     /locus_tag="ACHKBL_002621"
                     /EC_number="2.7.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HFO5819474.1"
                     /translation="MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAET
                     LIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRIVVDKEHYEVIAGVNIPMLVETL
                     MARDDDPSFDELVALAVETGREGVKALKAKPVEKAAPAPAAAAPKATPTPAKPMGPND
                     YMVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTA
                     HVVDVAKMIRVYNNPKYAGERVMLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQ
                     VNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISKIDK"
     gene            8909..9709
                     /gene="manY"
                     /locus_tag="ACHKBL_002622"
     CDS             8909..9709
                     /gene="manY"
                     /locus_tag="ACHKBL_002622"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HFO5819475.1"
                     /translation="MEITTLQIVLVFIVACIAGMGSILDEFQFHRPLIACTLVGIVLG
                     DMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASIISTILVIAGHQSIGAGIALAIPL
                     AAAGQVLTIIVRTITVAFQHAADKAADNGNLTAISWIHVSSLFLQAMRVAIPAVIVAL
                     SVGTSEVQNMLNAIPEVVTNGLNIAGGMIVVVGYAMVINMMRAGYLMPFFYLGFVTAA
                     FTNFNLVALGVIGTVMAVLYIQLSPKYNRVAGAPAQAAGNNDLDNELD"
     gene            9722..10573
                     /gene="manZ"
                     /locus_tag="ACHKBL_002623"
     CDS             9722..10573
                     /gene="manZ"
                     /locus_tag="ACHKBL_002623"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HFO5819476.1"
                     /translation="MVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCF
                     SMVPAIRRLYPENNEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDG
                     AINGIKVGLMGPLAGVGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLVRLA
                     TRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVSR
                     ITDQTGKEHVTTVQTILDQLMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGY
                     ACGLLGL"
     gene            10628..11086
                     /gene="yobD"
                     /locus_tag="ACHKBL_002624"
     CDS             10628..11086
                     /gene="yobD"
                     /locus_tag="ACHKBL_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 family protein"
                     /protein_id="HFO5819477.1"
                     /translation="MTITDLVLILFIAALLAFAIYDQFIMPRRNGPTLLAIPLLRRGR
                     IDSVIFVGLIVILIYNNVTNHGALITTWLLSALALMGFYIFWIRVPKIIFKQKGFFFA
                     NVWIEYSRIKAMNLSEDGVLVMQLEQRRLLIRVRNIDDLEKIYKLLVSTQ"
     gene            11516..12082
                     /gene="mntP"
                     /locus_tag="ACHKBL_002625"
     CDS             11516..12082
                     /gene="mntP"
                     /locus_tag="ACHKBL_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HFO5819478.1"
                     /translation="MNITATVLLAFGMSMDAFAASIGKGATLHKPKFSEALRTGLIFG
                     AVETLTPLIGWGMGMLASRFVLEWNHWIAFVLLIFLGGRMIIEGFRGADDEDKEPRRR
                     HGFWLLVTTAIATSLDAMAVGVGLAFLQVNIIATALAIGCATLIMSTLGMMVGRFIGS
                     IIGKKAEILGGLVLIGIGVQILWTHFHG"
     gene            complement(12079..12888)
                     /gene="rlmA"
                     /locus_tag="ACHKBL_002626"
     CDS             complement(12079..12888)
                     /gene="rlmA"
                     /locus_tag="ACHKBL_002626"
                     /EC_number="2.1.1.187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(745)-N(1))-methyltransferase"
                     /protein_id="HFO5819479.1"
                     /translation="MSFSCPLCHQPLSREKNSYICPQRHQFDMAKEGYVNLLPVQHKR
                     SRDPGDSAEMMQARRAFLDAGHYQPLRDAIVAQLRERLDEKATAVLDIGCGEGYYTHA
                     FADALPEITTFGLDVSKVAIKAAAKRYPQVTFCVASSHRLPFSDTSMDAIIRIYAPCK
                     AEELARVVKPGGWVITATPGPRHLMELKGLIYNEVHLHAPHAEQLEGFTLQQSDELCY
                     PMRLRGDEAVALLQMTPFAWRAKPEVWQTLAAKEVFDCQTDFNIHLWQRSY"
     gene            complement(13054..13263)
                     /gene="cspE"
                     /locus_tag="ACHKBL_002627"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(13054..13263)
                     /gene="cspE"
                     /locus_tag="ACHKBL_002627"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HFO5819480.1"
                     /translation="MAKIKGQVKWFNESKGFGFITPADGSKDVFVHFSAIQGNGFKTL
                     AEGQNVEFEIQDGQKGPAAVNVTAI"
     gene            complement(13276..13419)
                     /gene="yobF"
                     /locus_tag="ACHKBL_002628"
     CDS             complement(13276..13419)
                     /gene="yobF"
                     /locus_tag="ACHKBL_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2627 domain-containing protein"
                     /protein_id="HFO5819481.1"
                     /translation="MCGIFSKEVLSKHVDVEYRFSAEPYIGASCSNVSVLSMLCLRAK
                     KTI"
     gene            complement(14089..14376)
                     /gene="yebO"
                     /locus_tag="ACHKBL_002629"
     CDS             complement(14089..14376)
                     /gene="yebO"
                     /locus_tag="ACHKBL_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebO family protein"
                     /protein_id="HFO5819482.1"
                     /translation="MNEVVNSGVMNIASLVVSVVVLFIGLILWFFINRASSRTNEQIE
                     LLEALLDQQKRQNALLRRLCEANEPEKADKKTVESQKSVEDEDIIRLVAER"
     gene            complement(14451..14594)
                     /gene="mgrB"
                     /locus_tag="ACHKBL_002630"
     CDS             complement(14451..14594)
                     /gene="mgrB"
                     /locus_tag="ACHKBL_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP/PhoQ regulator MgrB"
                     /protein_id="HFO5819483.1"
                     /translation="MKKFRWVVLVVVVLACLLLWAQVFNMMCDQDVQFFSGICAINQF
                     IPW"
     gene            14753..14992
                     /gene="yobH"
                     /locus_tag="ACHKBL_002631"
     CDS             14753..14992
                     /gene="yobH"
                     /locus_tag="ACHKBL_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YobH family protein"
                     /protein_id="HFO5819484.1"
                     /translation="MRFIIRTVMLIALVWIGLLLSGYGVLIGSKENAAGLGLQCTYLT
                     ARGTSTVQYLHTKSGFLGITDCPLLRKSNIVVDNG"
     gene            complement(15136..15927)
                     /gene="kdgR"
                     /locus_tag="ACHKBL_002632"
     CDS             complement(15136..15927)
                     /gene="kdgR"
                     /locus_tag="ACHKBL_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator KdgR"
                     /protein_id="HFO5819485.1"
                     /translation="MANADLDKQPDSVSSVLKVFGILQALGEEREIGITELSQRVMMS
                     KSTVYRFLQTMKTLGYVAQEGESEKYSLTLKLFELGARALQNVDLIRSADIQMREISR
                     LTKETIHLGALDEDSIVYIHKIDSMYNLRMYSRIGRRNPLYSTAIGKVLLAWRDRDEV
                     KQILEGVEYKRSTERTITSTEALLPVLDQVREQGYGEDNEEQEEGLRCIAVPVFDRFG
                     VVIAGLSISFPTLRFSEERLQEYVAMLHTAARKISAQMGYHDYPF"
     gene            16104..17477
                     /gene="yebQ"
                     /locus_tag="ACHKBL_002633"
     CDS             16104..17477
                     /gene="yebQ"
                     /locus_tag="ACHKBL_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO5819486.1"
                     /translation="MPKVQADGLPLPQRYGAILTIVIGISMAVLDGAIANVALPTIAT
                     DLHATPASSIWVVNAYQIAIVISLLSFSFLGDMFGYRRIYKCGLVVFLLSSLFCALSD
                     SLQMLTLARVIQGFGGAALMSVNTALIRLIYPQRFLGRGMGINSFIVAVSSAAGPTIA
                     AAILSIASWKWLFLINVPLGIIALLLAMRFLPPNGSRASKPRFDLPSAVMNALTFGLL
                     ITALSGFAQGQSLTLIGAELVVMVVVGIFFIRRQLSLPVPLLPVDLLRIPLFSLSICT
                     SVCSFCAQMLAMVSLPFYLQTVLGRSEVETGLLLTPWPLATMVMAPLAGYLIERVHAG
                     LLGALGLFIMAAGLFSLVLLPASPADINIIWPMILCGAGFGLFQSPNNHTIITSAPRE
                     RSGGASGMLGTARLLGQSSGAALVALMLNQFGDNGTHVSLIAAAILAVIAACVSGLRI
                     TQPRSRA"
     gene            complement(17523..18404)
                     /gene="htpX"
                     /locus_tag="ACHKBL_002634"
     CDS             complement(17523..18404)
                     /gene="htpX"
                     /locus_tag="ACHKBL_002634"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014229823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HFO5819487.1"
                     /translation="MMRIALFLLTNLAVMVVFGLVLSLTGIQSSSVQGLMIMALLFGF
                     GGSFVSLLMSKWMALRSVGGEVIEQPRNERERWLVNTVATQARQAGIAMPQVAIYHAP
                     DINAFATGARRDASLVAVSTGLLQNMSPDEAEAVIAHEISHIANGDMVTMTLIQGVVN
                     TFVIFISRILAQLAAGFMGGNRDEGEESNGNPLIYFAVATVLELVFGILASIITMWFS
                     RHREFHADAGSAKLVGREKMIAALQRLKTSYEPQEATSMMAFCINGKSKSLSELFMTH
                     PPLDKRIEALRTGEYLK"
BASE COUNT         4617 a         4668 c         4467 g         4807 t
ORIGIN      
        1 ccatggatgt atcagcgcca acaccaagtt cgatgaatga aagtacggcg aagggaatct
       61 ttaaatattt aaaagaactg ggagtacccg cgagtgccgc tgatattacg gcgcgagctg
      121 accaggaagg ctggaaccct ggtttcacgg aaaaaatggt tggatgggca aaaaaaatgg
      181 agtctggtga acgctctgtg attaaaaatc ctgaatactt ttcaacatat atgcaggaag
      241 aactaaaagc actggtttga gttaaattta catcagcata aatgggtcag gacgctttta
      301 atcacatata aaaagcgtcc tttttctcac aatcaaataa tcatcggctt ctataacgaa
      361 agcgatacgg attagtgtgt tactcgtctt catcctcaaa acgcgccacg attcgctcgc
      421 cggcatggtt ggcacgcagc tcttctgcca ccaacgcgat tgcctggccg ctgctcatcc
      481 cttgagccat cagttcctgg atccgctcga cagctttttg ctgctgttca tgggtgagtg
      541 aaggtaaacc tgcaaacatt gttaactcct gctaaattgt tggcgctaat tatttcatgc
      601 tacccggcac atagccagta gagtcaggac tgatgaagac gttatctccc gctgtgatta
      661 ctttaccctg gcgtcaggac gccgctgaat tttatttctc acgcttaagc cacctgccgt
      721 gggcgatgct tttacactcc ggctatgctg atcatccata tagccgcttt gatattgtgg
      781 tcgccgagcc gatttgcact ttaaccactt tcggtaaaga aaccgttgtt agtgaaaacg
      841 aaaaacgcac aacgaccact gatgacccgc tacaggtgct ccagcaggtg ctggatcgcg
      901 cagacattcg cccagcgcat aacgaagatt tgccatttca gggcggcgcg ctggggttgt
      961 ttggctacga tctgggccgc cgttttgagt cactgccaga aattgcgcag caagatatcg
     1021 ttctgccgga tatggcagtg ggtatctacg actgggcgct ggttgttgac caccagcgtc
     1081 aaacagtttc tttgctgagt cataatgatg tcaatgctcg tcgggcctgg ctggaaagcc
     1141 agcaattctc gccgcaggaa ggtttcacgc tcacttccga ctggcaatcc aatatgaccc
     1201 gcgagcagta cggcgaaaaa tttcgccagg tacaggaata tctgcacagc ggtgattgct
     1261 atcaggtgaa tctcgcccag cgttttcatg cgacctattc tggcgatgaa tggcaggcat
     1321 tccttcagct taatcaggcc aaccgcgcgc catttagcgc ttttttacgt cttgaacagg
     1381 gtgcaatttt aagcctttcg ccagagcggt ttattctttg tgataatagt gaaatccaga
     1441 cccgcccgat taaaggcacg ctaccacgcc tgcccgatcc tcaggaagat agcaaacaag
     1501 cagaaaaact ggcgaactca gcgaaagatc gtgccgaaaa tctgatgatt gtcgatttaa
     1561 tgcgtaatga tatcggtcgc gttgccgtag ccggttcggt aaaagtacca gagctgttcg
     1621 tggtcgaacc cttccctgcc gtgcatcatc tggtcagcac cataacggcg cggctaccag
     1681 aacagttaca caccagcgat ctgctgcgcg cagcttttcc tggtggctca ataactgggg
     1741 ctccgaaagt acgggctatg gaaattatcg acgaactgga accgcaccga cgcaatgcct
     1801 ggtgcggcag cattggctat ttgagctttt gcggcaacat ggataccagc attactatcc
     1861 gcacgctaac tgccattaac ggacaaatat actgctctgc gggcggtgga attgtcgccg
     1921 atagccagga agaagcggaa tatcaggaaa cttttgataa agttaataag atcctacgtc
     1981 aactggagaa ataaacgtgg aacaccgtag cctgacgctt gatgattttt tatcgcgctt
     2041 tcaacttttg cgtccgcaaa tgaaccggga aacgttaaat catcgtcagg ctgccgtgtt
     2101 aatccccatc gtccgtcgac cgcaaccggg gttgttgctg actcagcgtt cgattcatct
     2161 gcgtaaacac gctggacaag tggcattccc tggaggcgca gtcgatgaca cggacgcatc
     2221 agttatcgcc gccgcgctgc gcgaagctga agaagaggtc gctataccgc cttccgccgt
     2281 tgaagttatc ggcgtgctgc cgcccgtcga tagcgtaact ggctaccagg taaccccagt
     2341 ggtcggcatt atcccgcccg atctgccgta tcgcgccagt gaagatgaag tctcggcggt
     2401 gtttgaaatg ccgctcgccc aggcattaca tctgggtcgt tatcatcctt tagatatcta
     2461 ccgccgtggc gattcacatc gggtatggct gtcctggtac gaacagtatt ttgtatgggg
     2521 aatgaccgca ggcataattc gtgagctggc gctgcaaatt ggtgtgaaac cctgactata
     2581 cttatcttta catctacaaa acactacttg agacaatcat cgcaatatta gttaaatcgc
     2641 ggtttttgat tagtttaatt catgtgaata gttaagccag tcgccgcgtt ccctcttaca
     2701 ctatgcgctg ttattagttc gttactggaa gtccagtcac cttgtcagga gtattatcgt
     2761 gattagtcta ttcgacatgt ttaaggtggg gattggtccc tcatcttccc ataccgtagg
     2821 gcctatgaag gcgggtaaac agttcgtcga tgatctggtc gaaaaaggct tactggatag
     2881 cgttactcgc gttgccgtgg acgtttatgg ttcactgtcg ctgacgggta aaggccacca
     2941 caccgatatc gccattatta tgggtcttgc aggtaacgaa cctgccaccg tggatatcga
     3001 cagtattccc ggttttattc gcgacgtaga agagcgcgaa cgtctgctgc tggcacaggg
     3061 acggcatgaa gtggatttcc cgcgcgacaa cgggatgcgt tttcataacg gtaaccttcc
     3121 gctgcatgaa aacggtatgc aaatccacgc ctataacggc gatgaagtcg tctacagcaa
     3181 aacttattat tccatcggcg gcggttttat cgtcgatgaa gaacactttg gtcaggatgc
     3241 tgccaacgaa gtgagcgtgc cgtatccgtt caaatctgcc accgaactgc tcgcgtactg
     3301 taatgaaacc ggctattcgc tttctggtct cgctatgcaa aacgaactgg cgctgcacag
     3361 caagaaagag atcgacgagt atttcgcgca tgtctggcaa accatgcagg catgtatcga
     3421 tcgcgggatg aataccgaag gcgtactgcc aggcccgttg cgcgtgccac gtcgtgcgtc
     3481 ggccctgcgc cggatgctgg tttccagcga taaactgtct aacgatccga tgaatgtcat
     3541 tgactgggta aacatgtttg cgctggcagt taacgaagaa aacgccgccg gtggccgtgt
     3601 ggtaactgcg ccaactaacg gtgcctgcgg tatcgttccg gcagtgctgg cttactatga
     3661 ccactttatt gaatcagtta gcccagacat ctatacccgt tactttatgg cagcgggcgc
     3721 gattggtgca ctgtataaaa tgaacgcgtc tatttccggt gcggaagtcg gttgccaggg
     3781 cgaagtaggt gttgcctgtt caatggctgc tgcgggcctt gccgagctgt tgggcggtag
     3841 cccggaacag gtttgtgtgg cagcggaaat tggcatggag cacaacctcg gtctgacctg
     3901 cgaccccgtt gcagggcaag ttcaggtgcc gtgcattgaa cgtaatgcca ttgcctctgt
     3961 gaaggcgatt aacgccgcac ggatggctct gcgccgcacc agtgcaccgc gcgtctcgct
     4021 ggataaggtc atcgaaacga tgtacgaaac cggtaaggac atgaacgcta aataccgcga
     4081 aacctcacgc ggtggtctgg caatcaaagt ccagtgtgac taatacttct tactcgccca
     4141 tctgcaacgg atgggcgaat ttattcccgc tttctcgtct gctgtaattt tccccactac
     4201 acttccactg ttgcgtcagg cgtttgtcgc catacgctta cagggtggcc cgcatgcaaa
     4261 aagcacaacg gatcattaaa acctatcgcc gtaatcgaat gattgtttgt acgatttgcg
     4321 cactcgttac gctcgcttcg accctgagcg tgcgatttat ttcacagcgt aacttaaatc
     4381 aacaacgggt agtacaattc gccaatcacg ctgtagagga attagataaa gtactgcttc
     4441 ccctacaggc aggtagcgaa gtcttgcttc cgctgattgg tctgccctgc tctgtcgccc
     4501 atttgccatt acgtaaacag gcggcaaaac tccaaactgt gcgatccatt ggcctggtgc
     4561 aagacggcac actttattgc tccagcattt ttggttatcg caatgtgccc gtcgtggaca
     4621 ttctggctga acttcctgca ccgcaaccac ttttacgcct gacgaccgac catgccctga
     4681 ttaaaggcag tccggttttg attcaatgga cgccagcagc gggcagtagc aaagctgggg
     4741 tcatggagat gattaacatc gacttactgg cggcaatgct gcttgagcca caactgccgc
     4801 aaatcagtag cgccagcctg acggtggacg atcggcattt gctctatggt aatgggctgg
     4861 tagattccct tccgcaacct gaaaacaatg aaaactatca ggtttcttcg caacgctttc
     4921 cttttaccat taacgttaat ggtccggggg ctacggcgct ggcatggcac tatcttccaa
     4981 cacaattacc gctggcggtg ctgctaagtt tacttgtggg ctatatcgcc tggctggcga
     5041 ccgcttaccg gatgagtttt tcccgcgaaa tcaatctggg cctggcgcaa catgagttcg
     5101 aattgttctg ccagcctttg cttaatgcgc gcagccagca atgtattggc gtagagattt
     5161 tgctgcgctg gaacaatccg cgtcagggct ggatttcgcc ggatgtgttt attcctatcg
     5221 cggaagaaca tcatttaatt gtgccactga cccgctatgt gatggcagaa accattcgtc
     5281 agcgccatgt tttcccgatg agtagtcagt ttcatgttgg cattaacgtc gcacccagcc
     5341 attttcgccg tggtgtgctg ataaaagatc tcaatcagta ctggtttagc gctcacccga
     5401 ttcagcaact gatcctcgaa atcaccgaac gcgatgcctt actggatgtt gattatcgga
     5461 ttgcccgcga gctacatcgt aaaaacgtca aactggcgat tgatgacttc ggcaccggca
     5521 atagttcatt ttcctggctt gaaacattac gtcctgacgt gctgaaaatt gataagtcat
     5581 ttaccgcagc tataggttct gacgcggtta actcgacggt gaccgatatc atcatcgcac
     5641 tggggcaaag actgaatatt gaactggtgg cggagggtgt ggaaacgcaa gaacaggcga
     5701 aatatttgct ccgtcatggc gtccatattt tgcaagggta tttgtacgca cagccgatgc
     5761 cactacgtga ttttcccaaa tggctggcgg gcagccaacc gccgcccgcc cggcataatg
     5821 gacatatcac acccattatg ccgttacgtt aacgcttact catcttcatc gtgcgccggt
     5881 tgctctttaa caatgcgaac cagatcaaca cgataatcat tggcttcaat gatggtgata
     5941 tgcagtggcc ctacatcaat cacatcgccc acacggggaa tgtgaccatt tgccgagatc
     6001 acgaggcccg cgaccgtcgc gatatcatcg tcatcggcaa ggtgctcaac atcaagcgcc
     6061 tgctgcaagg catgcaaatc tgtaccgcct tttaccagcc agccgtcacc gtcggtaatg
     6121 atttccggcg tttcgtcagc gtccgggaat tcacccgcaa tggcttccag cacatccagc
     6181 ggcgtgacca agccttgtac cacaccaaac tcgttggtca cgataacaaa gctcccgcga
     6241 gcacgacgca gcacgcccag caggttgatc ggatcgaggg tttccgggac gataatcgcc
     6301 ggagacgccg aagcaatcgc cgccacatca acgccctctt ccagcgccac cagcagttct
     6361 ttagcacgta cgattccgat gatttcatcc agttcaccgc gacataccgg gaacaggctg
     6421 tgcggtgagg agagcagctg ctcgcggatt tcatctaccc cgagattagc gtcaacccag
     6481 cttatttcac cgcgcggcgt catgatcccg cgcagagaac gcgacgccag cgtcagtacg
     6541 ccgttaatca tgtaacgttc ttcttcggca aatgcacctt ccgggatcgg catcggcatc
     6601 gggttatcgg catcgtgctg aacattggcc tgacgtttcc cgcccatcaa acgcaggatg
     6661 gcatcggcag tacgcgctcg cagcggcaaa gtcgactggt gacgaataaa gttgcgacgc
     6721 gcaatctggt taaacacttc gatgatgatc gagaagccaa tcgcggcata caggtaacct
     6781 ttcggaatgt ggaaaccgaa accttctgcc accagactca gaccaatcat taacaggaag
     6841 ctcagacaga gcaccaccac cgtggggtgc tggttaacaa atcgcgtcag cggtttcgat
     6901 gccagcagca taactgccat cgcaatcact accgccgcca tcatcaccgg caaatggtta
     6961 accatcccta ctgcagtaat taccgcatcc aacgagaaga cggcgtcaag gatgacaatc
     7021 tgtgtgacga ccacccagaa actggcgtag cctttaccgt ggccggaatc atgatcgcgg
     7081 ttttccagcc gttcatgcag ttcggttgtt gctttgaaca gcaagaatat gcccccgaac
     7141 aacataatca ggtcgcgtcc ggagaaggag aaatccatga cggtaaatag cggtttggtc
     7201 agcgtgacca tccatgaaat cagcgacagc agccccagac gcataatcag cgccagtgat
     7261 aatcccagca aacgcgcttt atcgcgttgt tttggcggca gtttgtcagc aagaatggca
     7321 atgaagacca ggttatcgat acccagcaca atttcgagaa caacaagcgt gagtagcccc
     7381 gcccaaattg aggggtccat taagaattcc atgacaagct cctgcttaag gaatagctat
     7441 tcgacgccag aaataatgca ggcgtaacga caaaatgcaa acgaaaggtg cggcatagag
     7501 tgccagaaag gcaggcgtta aaaggcctga tgctgaaatg acgtcggtga cgatccatac
     7561 tgcgggctac tgccctatac tccatggttg ttaaacggga gttaaacata tcagagacgc
     7621 ctctgatttg gcaaagattt accttccttt gcaaacgaat gtgacaagga tattttacct
     7681 ttcgaaattt ctgctaatcg aaagttaaat tacggatctt catcacataa aataattttt
     7741 tcgatatcta aaataaatcg cgaaacgcag gggtttttgg ttgtagccct tatctgaatc
     7801 gattcgattg tggacgacga ttcaaaaata catctggcac gttgaggtgt taacgataat
     7861 aaaggaggta gcaagtgacc attgctattg ttataggcac acatggttgg gctgcagagc
     7921 agttgcttaa aacggcagaa atgctgttag gcgagcagga aaacgtcggc tggatcgatt
     7981 tcgttccagg tgaaaatgcc gaaacgctga ttgaaaagta caacgctcag ttggcaaaac
     8041 tcgacaccac taaaggcgtg ctgtttctcg ttgatacatg gggaggcagc ccgttcaatg
     8101 ctgccagccg cattgtcgtc gacaaagagc attatgaagt cattgcaggc gttaacattc
     8161 caatgctcgt ggaaacgtta atggcccgtg atgatgaccc aagctttgat gaactggtgg
     8221 cgctggcagt agaaacaggt cgtgaaggcg tgaaagcact gaaagccaaa ccggttgaaa
     8281 aagccgcgcc agcacccgct gccgcagcac caaaagcgac tccaactccg gcaaaaccaa
     8341 tgggaccaaa cgactacatg gttattggcc ttgcgcgtat cgacgaccgt ctgatacacg
     8401 gtcaggtcgc cacccgctgg accaaagaaa ccaatgtctc ccgtattatt gttgttagtg
     8461 atgaagtggc tgcggatacc gttcgtaaga cactgctcac ccaggttgca cctccgggcg
     8521 taacagcaca cgtagttgat gttgccaaaa tgattcgcgt ctacaacaac ccgaaatatg
     8581 ctggcgaacg cgtaatgctg ttatttacca acccaacaga tgtagagcgt ctcgttgaag
     8641 gcggcgtgaa aatcacctct gttaacgtcg gtggtatggc attccgtcag ggtaaaaccc
     8701 aggtgaataa cgcggtttcg gttgatgaaa aagatatcga ggcgttcaag aaactgaatg
     8761 cgcgcggtat tgagctggaa gtccgtaagg tttccaccga tccgaaactg aaaatgatgg
     8821 atctgatcag caaaatcgat aagtaacgta ttgtgttgat tatcactcag ttttcacact
     8881 taagtcttac gtaaacagga gaagtacaat ggagattacc actcttcaaa ttgtgctggt
     8941 atttatcgta gcctgtatcg caggtatggg atcaatcctc gatgaatttc agtttcaccg
     9001 tccgctaatc gcgtgtaccc tggtgggtat cgttcttggc gatatgaaaa ccggtattat
     9061 tatcggtggt acgctggaaa tgatcgcgct gggctggatg aacatcggtg ctgcagttgc
     9121 gcctgacgcc gctctggctt ccatcatttc tactattctg gttatcgcag gtcatcagag
     9181 cattggtgca ggtatcgcac tggcaatccc tctggccgct gcgggccagg tactgaccat
     9241 catcgttcgt actattaccg ttgccttcca gcacgctgcg gataaggctg ctgataacgg
     9301 caacctgaca gcgatttcct ggatccacgt ttcttctctg ttcctgcaag caatgcgtgt
     9361 ggctattccg gccgtcatcg ttgcgctgtc tgttggtacc agcgaagtcc agaacatgct
     9421 gaatgcgatt ccggaagtgg tgaccaatgg tctgaatatc gccggtggca tgatcgtggt
     9481 ggttggttat gcgatggtta tcaacatgat gcgtgctggc tacctgatgc cgttcttcta
     9541 cctcggcttc gtaaccgcag cattcaccaa ctttaacctg gttgctctgg gtgtgattgg
     9601 tactgttatg gcagtgctct acatccaact tagcccgaaa tacaaccgcg tagccggtgc
     9661 gcctgctcag gcagctggta acaacgatct cgataacgaa ctggactaac aggtgagcga
     9721 aatggttgat acaactcaaa ctaccaccga gaaaaaactc actcaaagtg atattcgtgg
     9781 cgtcttcctg cgttctaacc tcttccaggg ttcatggaac ttcgaacgta tgcaggcact
     9841 gggtttctgc ttctctatgg taccggcaat tcgtcgcctc taccctgaga acaacgaagc
     9901 tcgtaaacaa gctattcgcc gtcacctgga gttctttaac acccagccgt tcgtggctgc
     9961 gccgattctc ggcgtaaccc tggcgctgga agaacagcgt gctaatggcg cagagatcga
    10021 tgacggtgct atcaacggta tcaaagtcgg tttgatgggg ccgctggctg gtgtaggcga
    10081 cccgatcttc tggggaaccg tacgtccggt atttgcagca ctgggggccg gtatcgcaat
    10141 gagcggcagc ctgttaggtc cgctgctgtt cttcatcttg tttaacctgg tgcgtctggc
    10201 aacccgttac tacggcgtag cgtatggtta ctccaaaggt atcgatatcg ttaaagatat
    10261 gggtggtggc ttcctgcaaa aactgacgga aggggcgtct atcctcggcc tgtttgtcat
    10321 gggggcatta gttaacaagt ggacgcatgt caacatcccg ctggttgtct ctcgcattac
    10381 tgaccagacg ggcaaagaac acgttaccac tgtccagact attcttgacc agttaatgcc
    10441 aggcctggta ccactgctgc tgacctttgc ttgtatgtgg ctgttgcgca aaaaagttaa
    10501 cccgctgtgg atcatcgttg gcttcttcgt catcggtatc gctggttacg cttgcggcct
    10561 gctgggactg taagactgtt gtacactacc ggggcctttt ggccccgttt ttttatctgg
    10621 aggattaatg acaatcacgg acctggtact gattcttttc atcgccgcac tcctggcctt
    10681 cgcgatctac gatcagttca tcatgccccg ccgtaacggc cccaccctgc tggcaattcc
    10741 tttgctccgg cgtggtcgca tcgatagcgt tatcttcgtc ggattgattg tcattcttat
    10801 ctataacaac gtcacgaatc atggcgcgct aataacaaca tggttattaa gtgcactggc
    10861 tctgatgggt ttttatatat tctggatccg cgttccgaag atcatcttta aacaaaaagg
    10921 ttttttcttc gccaatgtct ggattgaata tagccgaatc aaagcgatga acttgtcgga
    10981 agatggcgtg ttggtgatgc aattagaaca gcgccggctg ttaatccgcg ttcgaaatat
    11041 cgacgatctg gaaaaaattt ataagcttct cgtttcaact caataagtta tgaatttagc
    11101 caaagctatg tttagtgtat ttttaataat cagacatagc ttaggctata ttacctcttc
    11161 ccttatttgt tatttatttt aacgtttcat tgatatataa atctaaatga aaatcgttat
    11221 caataaagca atgaaataat atattccaac agttgtttta tattctcaaa atatgttaag
    11281 gttgcgccct catttgggga gtagccgatt tccagattcc ggaaatgtac gtgtcaacat
    11341 actcgttgca aaacgtggca cgtacggact gaacactttc agtcaggcga gaccatatgc
    11401 acatcaatcg ctatgcctgc atgaacgcga aaacccatcg cggcttgttg ttcatgtatg
    11461 catgtttgct gggggcgatg atgtgtttta tggatacccc ggtcaggacg ttgtcatgaa
    11521 tatcactgct actgttcttc ttgcgtttgg tatgtcgatg gatgcatttg ctgcatcaat
    11581 cggtaaaggt gccaccctcc ataaaccgaa attttctgaa gcattgcgaa ccggccttat
    11641 ttttggtgcc gtcgaaaccc tgacaccgct gatcggctgg ggaatgggca tgttagccag
    11701 ccggtttgtc cttgaatgga atcactggat tgcgtttgtg ctgctgatat tcctcggcgg
    11761 gcgaatgatt attgagggtt ttcgtggcgc agatgatgaa gataaagagc cgcgccgtcg
    11821 gcacggtttc tggctactgg taaccaccgc gattgccacc agcctggatg ccatggctgt
    11881 gggtgttggt ctcgccttcc tgcaggtcaa cattatcgcg accgcattgg ccattggttg
    11941 tgcaaccttg attatgtcga cattagggat gatggttggt cgctttatcg gctcaattat
    12001 tgggaaaaaa gcggaaattc tcggcgggct ggtgctgatc ggcatcggcg tccagatcct
    12061 ctggacgcac ttccacggtt aataagaacg ctgccagagg tgaatattaa agtccgtctg
    12121 gcagtcgaac acttcttttg ctgccagtgt ttgccagact tctggcttcg cacgccaggc
    12181 aaacggcgtc atctgcaata atgcgacggc ttcatcaccg cgaagacgca tcggataaca
    12241 caactcatca ctctgctgta atgtaaaacc ttccagttgt tctgcatgtg gtgcatgaag
    12301 atgtacttca ttgtaaatca gccccttcag ctccatcaaa tgtcgcggtc ccggcgtggc
    12361 agtaatgacc cagccgccag gcttcactac tcgcgctaat tcttctgctt tacacggcgc
    12421 gtaaatacgt attatggcgt ccatactagt atcggaaaac ggcaaacggt ggctggaagc
    12481 aacacaaaaa gtgacctgcg gatagcgttt cgccgccgct tttatcgcta ccttcgagac
    12541 atccaggcca aacgtggtga tttcgggcag cgcatcggca aatgcgtgtg tgtaataccc
    12601 ttcaccacag ccaatatcca gcaccgctgt ggccttttca tcaagccgtt cccgcagttg
    12661 ggcgacaatt gcatcacgca gcggctgata atgtccggca tctaagaatg cgcggcgtgc
    12721 ttgcatcatt tccgcgctgt cgcccggatc acgagaccgt ttatgctgaa cgggcagcag
    12781 attgacatac ccttctttcg ccatatcaaa ctgatgtcgc tggggacaga tatagctgtt
    12841 tttttcacgc gaaagaggct gatggcaaag tggacaggaa aacgacatga caactccggc
    12901 aggatactaa aggccgcaag tgtaacgcga atcacgcggc gggcaaatat ccgaacgcca
    12961 ggagcgaaga taaagcaaag taaaaagcct cgcattcgac gaggctttat agcgagattg
    13021 aagcgtattc acacttcaga tcagtggatt cgatcagata gctgttacgt taacagctgc
    13081 cggacctttc tgaccgtcct gaatttcgaa ctcaacgttc tgaccttcag ccagagtttt
    13141 gaaaccatta ccctggatag cagagaagtg tacgaacacg tctttgctgc catcagccgg
    13201 agtaatgaag ccaaaacctt tagactcgtt gaaccactta acctgacctt taatctttgc
    13261 catttgaaaa attccttaga ttgttttctt cgcccgcagg cataacatag ataaaactga
    13321 cacattactg catgaggcac caatataagg ctcggcagag aagcggtatt caacgtcaac
    13381 gtgtttactc aggacttctt tactgaaaat gccacacata aacagaactg tacctcgttt
    13441 aacccgaaat ctgttatcac atacaacgtt aattatggca agccattttt aaacatgtct
    13501 cgatcagacg cacaaatctc gatacgtctt ccactttttt tgcacactta tgcaacggaa
    13561 tacgcgccga tatatcattg tgcttaacct tgccagttca ggcagatact taaactgacg
    13621 tattttctaa catagttcaa taagttcagt tgttccaggc cagtgggaga agttattaca
    13681 tagtgcgtgc aatatcacat tttctgctat gcaatgaata aaaagttata tcactttttc
    13741 tcataaaaca gtcagttaac ggccaccaat taccccaaag agaagtcaat ccccaatggt
    13801 attgtaaatt taaaataaga aaaactgatg aatgcgcaaa aaaatcaaga gagaaacgtt
    13861 tcgctaataa ataataccac gcttaatcac aaatccgatg taaacatcct aataaattaa
    13921 tgggctaaat atctttttct gatatcccgt tacgaaaata aataatatga taattatttg
    13981 aagtcagacc agagttttaa tttgacgcag ttgcaccatg acagtgcgta taatcaggat
    14041 gaattgagta acccttcaac gacggggtta ctcaagattg aggaaagatt atcgttcggc
    14101 gaccagacga ataatatctt cgtcttcaac cgatttttga ctctcaacgg tctttttatc
    14161 tgctttctct ggttcgtttg cttcgcacaa acgacgtaac agagcatttt gacgtttttg
    14221 ctgatccagc aacgcctcaa gcagttcaat ctgttcgtta gtccgtgaac tggcacgatt
    14281 gataaaaaac cacaagataa gcccgataaa aagaaccacc accgatacaa ccaaagacgc
    14341 aatgttcatc acgcctgaat ttacaacttc gttcatttca ccacctctga gtagaggcgc
    14401 tattctacca ctgctggaga ggaagaaaat ctagtgctga aaaaatgata tcaccacggg
    14461 ataaactggt taatggcaca aattccgctg aaaaattgta catcctgatc gcacatcatg
    14521 ttgaatacct gcgcccaaag cagcaagcaa gccaacacca cgacaaccag aacgacccat
    14581 cgaaactttt tcactccaat ctccgtttca cctaccttat gtcattaaac tagcatgcgt
    14641 tagttaaaca gcgcctaact atgtcgatat tcgtgctttt tcagaatgag tcgcttgctt
    14701 cagcgcatat tgccgctacg attaagcgaa cataaaaaga gaagaggttg taatgcgatt
    14761 catcattcgc acagttatgc tgatagcact ggtatggata ggattattac tcagcggata
    14821 tggcgttttg attggaagca aagagaatgc cgcgggatta ggtttgcaat gtacctatct
    14881 gaccgccaga ggcaccagca cggtacaata cctgcacact aaaagtggtt ttctggggat
    14941 aacagattgc ccgttgttac gtaaaagcaa tattgtggtc gataatggtt aagggtaata
    15001 aataaccaga ttctcactca tgagtaaaat attcttatcg caagtgcgtt taatataacg
    15061 ccaccttcaa ctttattttt ctcatgctaa ataaagaacc tgtctgtttg atattcagac
    15121 aggttcttac tctcatcaga acggatagtc gtgataaccc atttgggcag aaattttgcg
    15181 cgccgcggta tgcaacattg cgacatattc ctgtaaacgc tcttcagaga aacgtaacgt
    15241 cgggaaggag atgctcaaac cggcaatgac cacgccaaag cgatcaaata ccggaaccgc
    15301 aatgcatcgc agcccttctt cctgctcttc gttatcttcg ccatacccct gctcgcgcac
    15361 ctggtccaga acgggtaata acgcttctgt actggtgatg gtccgctcgg tactgcgttt
    15421 atactccacg ccctcaagaa tttgcttcac ttcatcgcga tcgcgccatg ccagcagtac
    15481 cttaccaatc gcggtgctgt acagcggatt acgacgccca atccgtgaat acatgcgcaa
    15541 attgtacata gagtcaattt tgtgaatgta aacaatactg tcttcgtcca gtgcgccgag
    15601 gtggatagtt tctttggtca ggcgggagat ctcacgcatc tggatatctg cgctacgaat
    15661 taaatcgacg ttttgtaacg cgcgagcgcc cagttcaaac aatttcaggg tcagcgaata
    15721 tttctccgac tccccttcct gcgccacata acctaaggtt ttcatggtct gtaaaaagcg
    15781 ataaacggtg ctttttgaca tcatgacgcg ctgcgacaac tcggttatcc ctatttcgcg
    15841 ctcttcaccc agcgcctgca aaatgccaaa aacttttagc acggaagata cagaatcagg
    15901 ctgtttatcc agatctgcgt tagccattta tcacctcatt gcgagtgttt tataaaaatc
    15961 agaactgttt tttattataa tttcgcacca gggtggtcgc aatccatctt ttgccggtta
    16021 gttacaattc tgcgacatcc accgtgaata tcagtgctag aatcataccc ctgttgatta
    16081 ttcaccaaag atataaaatt cctatgccaa aagttcaggc cgacggcctg ccattgcccc
    16141 agcgatacgg tgcgatatta accattgtga ttggtatttc gatggctgtc cttgacggcg
    16201 caatcgccaa cgtcgccttg ccaacaatcg ccacggacct tcatgccacg ccagccagtt
    16261 ccatctgggt agtgaacgcc tatcaaatcg ccattgtcat ctccctgctc tcgttttcgt
    16321 ttctgggcga tatgtttggc tatcgacgta tttataaatg cggtctggtc gtttttctgt
    16381 tgtcttcact attctgcgcc ctttctgatt cgctgcaaat gctcaccctt gcgcgtgtca
    16441 tacaaggttt cggcggtgca gcgttgatga gcgttaatac cgcacttatc cgcctgatct
    16501 atccacaacg ttttctgggt agagggatgg gcataaactc gtttattgtt gccgtttctt
    16561 ctgctgccgg gccgacaatt gctgcagcaa tcctctccat cgcatcctgg aaatggttat
    16621 ttttaatcaa cgtaccgttg ggtattatcg ccctgcttct ggcgatgcgt tttctgccac
    16681 ccaatggttc tcgcgccagt aaaccccgtt tcgacctgcc cagcgccgtg atgaacgcgt
    16741 taaccttcgg cctgcttatt actgcattga gtggtttcgc tcaggggcaa tcgctgacat
    16801 tgattggtgc ggaactggtg gtaatggttg tcgttggtat tttctttatt cgccgccagc
    16861 tttctcttcc cgtaccgctg ctaccggtgg atttactgcg tatcccgctg ttttcacttt
    16921 ctatttgcac atctgtttgc tctttctgcg cacaaatgct ggcaatggtt tcccttccct
    16981 tttacctgca aaccgtgctc gggcgtagtg aagtcgaaac aggtttactt ctgacaccgt
    17041 ggccgttagc aacaatggtg atggctccac tggcaggcta tttgattgaa cgcgtacatg
    17101 caggattgct gggtgcttta gggttattca tcatggctgc ggggcttttt tccctagttc
    17161 tgctgccagc gtcacctgcg gatatcaata ttatctggcc gatgatctta tgtggtgctg
    17221 gatttggctt gttccagtca cccaataacc acaccattat tacctccgct cctcgcgaac
    17281 gtagcggtgg agccagtggc atgttaggga cggctcgtct tctgggtcag agtagcggcg
    17341 cggctctggt ggcgctgatg ctaaatcagt ttggtgataa tggtacgcac gtctcgctga
    17401 tagctgcggc tattctggcg gtgattgcag cctgtgtcag tggtttacgt atcactcagc
    17461 cacgatccag ggcataaaaa aagcgcgtcg atcaggacgc gctttttagt atttacttca
    17521 tattacttca ggtattcacc cgtacgcaga gcttcaatac gtttatccag tggcgggtga
    17581 gtcatgaaca actcactgag cgatttcgac ttcccgttaa tgcagaaagc catcatgctg
    17641 gttgcttctt gcggttcata gctggttttc aggcgctgca atgcagcaat cattttctcg
    17701 cgaccaacca gttttgctga acccgcatcg gcgtggaatt cacgatgacg cgagaaccac
    17761 atggtgataa tgctcgccag aataccaaac accagttcca gaaccgttgc aaccgcaaag
    17821 tagatcagcg ggttgccgtt gctctcttca ccttcatcac gatttccgcc cataaaaccc
    17881 gcggcaagct gcgccagaat acgggaaata aagataacga aggtgttcac cacgccctga
    17941 atcagcgtca tggtgaccat atcaccattg gcgatgtggc tgatttcgtg agcaattacc
    18001 gcctcggctt catccgggct catgttctgc agcaaaccgg tgctgacagc aaccagagag
    18061 gcatcacggc gcgcaccggt tgcaaaagca ttgatgtccg gcgcatggta gatagccact
    18121 tgcggcatag cgatccccgc ctgacgagcc tgggttgcta ccgtattgac cagccaacgt
    18181 tccctttcgt tacgcggttg ctcgatcact tccccgccaa cagatcgtaa tgccatccat
    18241 ttggacatca gaagcgaaac gaaggaacca ccaaaaccga acagcaaggc catgatcatc
    18301 agcccctgaa cgctgctcga ctgtatccct gtcaggctca gtaccagccc gaaaacgacc
    18361 attacggcca ggttcgttag caggaagagc gcgattcgca tcataatttt ctttttacct
    18421 cagtttaaca aaacgcaata tgcgataccc acatcgtatg ggttacgcga ctattttcaa
    18481 gtctggatag tgcgtaagtc accagaaaga cacaacttta cattttgtag catctgattt
    18541 acggcatctt gctgctgtt
//
LOCUS       DAZFLD010000091        18271 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.091, whole genome shotgun sequence.
ACCESSION   DAZFLD010000091 DAZFLD010000000
VERSION     DAZFLD010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18271
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.091"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            101..1321
                     /locus_tag="ACHKBL_002635"
     CDS             101..1321
                     /locus_tag="ACHKBL_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HFO5819488.1"
                     /translation="MSIYKIPLPLNILEAAKERITWTLNTLPRICVSFSGGKDSGLML
                     HLTAEIARQMGKKICVLFIDWEAQFSCTINYVQSLREFYADVIEEFYWVALPLTTQNS
                     LSQYQPEWQCWEPDVEWVRQPPQDAITDPDFFSFYQPGMTFEQFVREFAEWFSQKRPA
                     AMMIGIRADESYNRFVAIASLNKQRFADDMPWTTAAPGGHSWYIYPIYDWKVADIWTW
                     YANHQQLCNPLYNIMYQAGVPLRHMRICEPFGPEQRQGLWLYHVIEPDRWAAMCARVS
                     GVKSGGIYAGHDNHFYGHRKILKPEHLDWQEYALLLLNSMPEKTAEHYRNKIAIYLHW
                     YQKKGIEVPQTQQGDIGAKDIPSWRRICKVLLNNDYWCRALSFSPTKAKNYQRYNERI
                     KGKRQEWGILCNND"
     gene            1294..1923
                     /locus_tag="ACHKBL_002636"
     CDS             1294..1923
                     /locus_tag="ACHKBL_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA];
                     GO_process: GO:0043170 - macromolecule metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043412 - macromolecule modification
                     [Evidence IEA];
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IbrB-like domain-containing protein"
                     /protein_id="HFO5819489.1"
                     /translation="MGDTMQQRLTQDLTQFLASLPEDDRIKAINEIRMAIHQVSPFRE
                     EPVDCVLWVKNSQLIPNDYNPNNVAPPEKKLLKKSIETDGFTQPIVVTHTDKNALEIV
                     DGFHRHEIGKGSSSLKLRLKGYLPVTCLEGTRNQRIAATIRHNRARGRHQITAMSEIV
                     RELSQLGWDDNKIGKELGMDSDEVLRLKQINGLQELFADRQYSRAWTVK"
     gene            complement(1924..3084)
                     /gene="ybdL"
                     /locus_tag="ACHKBL_002637"
     CDS             complement(1924..3084)
                     /gene="ybdL"
                     /locus_tag="ACHKBL_002637"
                     /EC_number="2.6.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine-oxo-acid transaminase"
                     /protein_id="HFO5819490.1"
                     /translation="MTNNPLIPQSKLPQLGTTIFTRMSALAQQHQAINLSQGFPDFDG
                     PRYLQERLAYHVAQGANQYAPMTGVQALREAIAQKTERLYGYQPDADSDITVTAGATE
                     ALYAAITALVRNGDEVICFDPSYDSYAPAIALSGGIVKRIALQPPHFRVDWQEFAALL
                     SERTRLVILNTPHNPSATVWQQADFAALWQAIAGHEIFVISDEVYEHINFSQQGHASV
                     LAHPQLRERAVAVSSFGKTYHMTGWKVGYCVAPAPISAEIRKVHQYLTFSVNTPAQLA
                     LADMLRAEPEHYLALPDFYRQKRDILVNALSESRLEILPCEGTYFLLVDYSAVSSLDD
                     VKFCQWLTREHGVAAIPLSVFCADPFPHKLIRLCFAKKESTLLAAAERLRQL"
     gene            3193..4281
                     /gene="hcxA"
                     /locus_tag="ACHKBL_002638"
     CDS             3193..4281
                     /gene="hcxA"
                     /locus_tag="ACHKBL_002638"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcxA"
                     /protein_id="HFO5819491.1"
                     /translation="MPHNPIRVVVGPANYFSHPGSFNHLHDFFTDEQLSRAVWIYGER
                     AIAAAQTKLPPAFELPGVKHILFRGHCSESDVQQLAAESGDDRSVVIGVGGGALLDTA
                     KALARRLGLPFVAVPTIAATCAAWTPLSVWYNDAGQALHYEIFDDANFMVLVEPEIIL
                     NAPQEYLLAGIGDTLAKWYEAVVLAPQPETLPLTVRLGINNAQAIRDVLLNSSEQALS
                     DQQKQQLTQSFCDVVDAIIAGGGMVGGLGDRFTRVAAAHAVHNGLTVLPQTEKFLHGT
                     KVAYGILVQSALLGQDDVLAQLTEAYQRFHLPTTLAELEVDINNQVEIDKMIAHTLRP
                     VESIHYLPVTLTPDTLRAAFENVESFKA"
     gene            complement(4291..4488)
                     /locus_tag="ACHKBL_002639"
     CDS             complement(4291..4488)
                     /locus_tag="ACHKBL_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HFO5819492.1"
                     /translation="MFDSLAKAGKYLGQAAKLMIGMPDYDNYVEHMRVNHPDQTPMTY
                     EEFFRERQDARYGGKGGARCC"
     gene            complement(4601..6706)
                     /gene="cstA"
                     /locus_tag="ACHKBL_002640"
     CDS             complement(4601..6706)
                     /gene="cstA"
                     /locus_tag="ACHKBL_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter CstA"
                     /protein_id="HFO5819493.1"
                     /translation="MNKSGKYLVWTVLSVMGAFALGYIALNRGEQINALWIVVASVCI
                     YLIAYRFYGLYIAKNVLAVDPTRMTPAVRHNDGLDYVPTDKKVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGMIWLLAGVVLAGAVQDFMVLFVSTRRDGRSLGELVKEEMGPTAG
                     VIALVACFMIMVIILAVLAMIVVKALTHSPWGTYTVAFTIPLALFMGIYLRYLRPGRI
                     GEVSVIGLVFLIFAIISGGWVAESPTWAPYFDFTGVQLTWMLVGYGFVAAVLPVWLLL
                     APRDYLSTFLKIGTIVGLAVGILIMRPTLTMPALTKFVDGTGPVWTGNLFPFLFITIA
                     CGAVSGFHALISSGTTPKMLANEGQACFIGYGGMLMESFVAIMALVSACIIDPGVYFA
                     MNSPMAVLAPAGTADVVASAAQVVSSWGFAITPDTLNQIASEVGEQSIISRAGGAPTL
                     AVGMAYILHGALGGMMDVAFWYHFAILFEALFILTAVDAGTRAARFMLQDLLGVVSPG
                     LKRTDSLPANLLATALCVLAWGYFLHQGVVDPLGGINTLWPLFGIANQMLAGMALMLC
                     AVVLFKMKRQRYAWVALVPTAWLLICTLTAGWQKAFSPDAKVGFLAIANKFQAMIDSG
                     NIPSQYTESQLAQLVFNNRLDAGLTIFFMVVVVVLALFSIKTALVALKEPKPTAKETP
                     YEPMPENVEEIVAQAKGAH"
     gene            complement(6887..7300)
                     /gene="entH"
                     /locus_tag="ACHKBL_002641"
     CDS             complement(6887..7300)
                     /gene="entH"
                     /locus_tag="ACHKBL_002641"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proofreading thioesterase EntH"
                     /protein_id="HFO5819494.1"
                     /translation="MIWKRHLTLDELNATSDNTMVAHLGIVYTRLGDDVLEAEMPVDT
                     RTHQPFGLLHGGASAALAETLGSMAGFMMTRDGQCVVGTELNATHHRPVSEGKVRGVC
                     QPLHLGRQNQSWEIVVFDEQGRRCCTCRLGTAVLG"
     gene            complement(7303..8049)
                     /gene="entA"
                     /locus_tag="ACHKBL_002642"
     CDS             complement(7303..8049)
                     /gene="entA"
                     /locus_tag="ACHKBL_002642"
                     /EC_number="1.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase
                     EntA"
                     /protein_id="HFO5819495.1"
                     /translation="MDFSGKNVWVTGAGKGIGYATALAFVEAGAKVTGFDQAFTQEQY
                     PFATEVMDVADAAQVAQVCQRLLAETERLDVLVNAAGILRMGATDQLSKEDWQQTFAV
                     NVGGAFNLFQQTMNQFRRQRGGAIVTVASDAAHTPRIGMSAYGASKAALKSLALSVGL
                     ELAGSGVRCNVVSPGSTDTDMQRTLWVSDDAEEQRIRGFGEQFKLGIPLGKIARPQEI
                     ANTILFLASDLASHITLQDIVVDGGSTLGA"
     gene            complement(8049..8906)
                     /gene="entB"
                     /locus_tag="ACHKBL_002643"
     CDS             complement(8049..8906)
                     /gene="entB"
                     /locus_tag="ACHKBL_002643"
                     /EC_number="3.3.2.1"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis bifunctional
                     isochorismatase/aryl carrier protein EntB"
                     /protein_id="HFO5819496.1"
                     /translation="MAIPKLQAYALPESHDIPQNKVDWAFEPQRAALLIHDMQDYFVS
                     FWGENCPMMEQVIANIAALRDYCKQHNIPVYYTAQPKEQSDEDRALLNDMWGPGLTRS
                     PEQQKVVDSLTPDADDTVLVKWRYSAFHRSPLEQMLKESGRNQLIITGVYAHIGCMTT
                     ATDAFMRDIKPFMVADALADFSRDEHLMSLKYVAGRSGRVVMTEELLPAPIPASKAAL
                     REVILPLLDESDEPFDDDNLIDYGLDSVRMMALAARWRKVHGDIDFVMLAKNPTIDAW
                     WKLLSREVK"
     gene            complement(8920..10530)
                     /gene="entE"
                     /locus_tag="ACHKBL_002644"
     CDS             complement(8920..10530)
                     /gene="entE"
                     /locus_tag="ACHKBL_002644"
                     /EC_number="2.5.1.-"
                     /EC_number="6.2.1.71"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2,3-dihydroxybenzoyl)adenylate synthase EntE"
                     /protein_id="HFO5819497.1"
                     /translation="MSIPFTRWPEEFARRYREKGYWQDLPLTDILTRHAASDSIAVID
                     GERQLSYRELNQAADNLACSLRRQGIKPGETALVQLGNVAELYITFFALLKLGVAPVL
                     ALFSHQRSELNAYASQIEPALLIADRQHALFSGDDFLNTFVAEHSSIRVVQLLNDSGE
                     HNLQDAINHPAEDFTATPSPADEVAYFQLSGGTTGTPKLIPRTHNDYYYSVRRSVEIC
                     QFTQQTRYLCAIPAAHNYAMSSPGSLGVFLAGGTVVLAADPSATLCFPLIEKHQVNVT
                     ALVPPAVSLWLQALAEGESRAQLASLKLLQVGGARLSATLAARIPAEIGCQLQQVFGM
                     AEGLVNYTRLDDSAEKIIHTQGYPMCPDDEVWVADAEGNPLPQGEVGRLMTRGPYTFR
                     GYYKSPQHNASAFDANGFYCSGDLISIDPEGYITVQGREKDQINRGGEKIAAEEIENL
                     LLRHPAVIYAALVSMEDELMGEKSCAYLVVKEPLRAVQVRRFLREQGIAEFKLPDRVE
                     CVDSLPLTAVGKVDKKQLRQWLASRASA"
     gene            complement(10540..11715)
                     /gene="entC"
                     /locus_tag="ACHKBL_002645"
     CDS             complement(10540..11715)
                     /gene="entC"
                     /locus_tag="ACHKBL_002645"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase EntC"
                     /protein_id="HFO5819498.1"
                     /translation="MDTSLAEEVQQTMATLAPNRFFFMSPYRSFTTSGCFARFDEPAV
                     NGDSPDSPFQQKLAALFAGAKAQGIKNPVMVGAIPFDPRQPSSLYIPESWQSFSRQEK
                     QASARRFTRSQSLNVVERQAIPEQTTFEQMVARAAALTATPQVDKVVLSRLIDITTDA
                     AIDSGVLLERLIAQNPVSYNFHVPLADGGVLLGASPELLLRKDGERFSSIPLAGSARR
                     QPDEVLDREAGNRLLASEKDRHEHELVTQAMKEVLRKRSSELHVPSSPQLITTPTLWH
                     LATPFEGKANSQENALTLACLLHPTPALSGFPHQAATQVIAELEPFDRELFGGIVGWC
                     DSEGNGEWVVTIRCAKLRENQVRLFAGAGIVPASSPLGEWRETGVKLSTMLNVFGLH"
     gene            11904..12860
                     /gene="fepB"
                     /locus_tag="ACHKBL_002646"
     CDS             11904..12860
                     /gene="fepB"
                     /locus_tag="ACHKBL_002646"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-enterobactin ABC transporter
                     substrate-binding protein"
                     /protein_id="HFO5819499.1"
                     /translation="MRLVPLYRNALLLTGLLLSGIAVVQAADWPRQITDSRGTHTLES
                     QPQRIVSTSVTLTGSLLAIDAPVIASGATTPNNRVADDQGFLRQWSKVAKERKLQRLY
                     IGEPSAETVAAQMPDLILISATGGDSALALYDQLSTIAPTLIINYDDKSWQSLLTQLG
                     EITGHEKQAAERIAQFDKQLAAAKEQIKLPPQPVTAIVYTAAAHSANLWTPESAQGQM
                     LEQLGFTLAKLPAGLNASQSQGKRHDIIQLGGENLASGLNGESLFLFAGDQKDADAIY
                     ANPLLAHLPAVQNKQVYALGTETFRLDYYSAMQVLERLKALF"
     gene            complement(12864..14114)
                     /gene="entS"
                     /locus_tag="ACHKBL_002647"
     CDS             complement(12864..14114)
                     /gene="entS"
                     /locus_tag="ACHKBL_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042931 - enterobactin transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0042930 - enterobactin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin transporter EntS"
                     /protein_id="HFO5819500.1"
                     /translation="MNKQSWLLNLSLLKTHPAFRAVFLARFISIVSLGLLGVAVPVQI
                     QIMTHSTWQVGLSVTLTGGAMFVGLMVGGVLADRYERKKVILLARGTCGIGFIGLCLN
                     ALLPEPSLLAIYLLGLWDGFFASLGVTALLAATPALVGRENLMQAGAITMLTVRLGSV
                     ISPMIGGLLLATGGVAWNYGLAAAGTFITLLPLLSLPALPPPPQPREHPLKSLLAGFR
                     FLLASPLVGGIALLGGLLTMASAVRVLYPALADNWQMSAAQIGFLYAAIPLGAAIGAL
                     TSGKLAHSARPGLLMLLSTLGSFLAIGLFGLMPMWILGVVCLALFGWLSAVSSLLQYT
                     MLQTQTPEAMLGRINGLWTAQNVTGDAIGAALLGGLGAMMTPVASASASGFGLLIIGV
                     LLLLVLVELRRFRQTPPQVTASDS"
     gene            14225..15229
                     /gene="fepD"
                     /locus_tag="ACHKBL_002648"
     CDS             14225..15229
                     /gene="fepD"
                     /locus_tag="ACHKBL_002648"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-siderophore ABC transporter permease"
                     /protein_id="HFO5819501.1"
                     /translation="MSGSVAVTRAIAVPGLLLLLIIATALSLLIGAKSLPASVVLEAL
                     SGTCQSADCTIVLDARLPRTLAGLLAGGALGLAGALMQTLTRNPLADPGLLGVNAGAS
                     FAIVLGAALFGYSSAQEQLAMAFAGALVASLIVAFTGSQGGGQLSPVRLTLAGVALAA
                     VLEGLTSGIALLNPDVYDQLRFWQAGSLDIRNLYTLKVVLIPVLIAGATALLLSRALN
                     SLSLGSDTATALGSRVARTQLIGLLAITVLCGSATAVVGPIAFIGLMMPHMARWLVGA
                     DHRWSLPVTLLATPALLLFADIIGRVIVPGELRVSVVSAFIGAPVLIFLVRRKTRGGA
                     "
     gene            15226..16218
                     /gene="fepG"
                     /locus_tag="ACHKBL_002649"
     CDS             15226..16218
                     /gene="fepG"
                     /locus_tag="ACHKBL_002649"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter permease"
                     /protein_id="HFO5819502.1"
                     /translation="MIYVSRRLIITCLLLVSACVVAGIWGLRSGAITLETSQVFAALM
                     GDAPRSMTMVVTEWRLPRVLMALLIGAALGVSGAIFQSLMRNPLGSPDVMGFNTGAWS
                     GVLVAMVLFGQDLTAIALAAMAGGIVTSLLVWLLAWRNGIDTFRLIIIGIGVRAMLVA
                     FNTWLLLKASLETALTAGLWNAGSLNGLTWAKTSPSAPIIILMLIAASLLVRRMRLLE
                     MGDDTACALGVSVERSRLLMMLVAVVLTAAATALAGPISFIALVAPHIARRISGTARW
                     GLTQAALCGALLLLVADLCAQLLFMPYQLPVGVVTVSLGGIYLIVLLIQESRKK"
     gene            16215..17030
                     /gene="fepC"
                     /locus_tag="ACHKBL_002650"
     CDS             16215..17030
                     /gene="fepC"
                     /locus_tag="ACHKBL_002650"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter ATP-binding
                     protein"
                     /protein_id="HFO5819503.1"
                     /translation="MTESVARLRGEQLTLGYGKYTVAENLTVEIPDGHFTAIIGPNGC
                     GKSTLLRTLSRLMTPAHGHVWLDGEHIQHYASKEVARRIGLLAQNATTPGDITVQELV
                     ARGRYPHQPLFTRWRKEDEDAVTKAMQATGITHLADQSVDTLSGGQRQRAWIAMVLAQ
                     ETAIMLLDEPTTWLDISHQIDLLELLSELNREKGYTLAAVLHDLNQACRYASHLIALR
                     EGKIVAQGAPKEIVTAELIERIYGLRCMIIDDPVAGTPLVVPLGRTTPSTAKI"
     gene            complement(17027..18160)
                     /gene="wzz(fepE)"
                     /locus_tag="ACHKBL_002651"
     CDS             complement(17027..18160)
                     /gene="wzz(fepE)"
                     /locus_tag="ACHKBL_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator Wzz(fepE)"
                     /protein_id="HFO5819504.1"
                     /translation="MSSLNIKQGSEAHFPEYPLASPSNNEVDLLNLIEVLWRAKKTVM
                     AVVFAFACAGLLISFILPQKWTSAAVVTPPEPVQWQALEKTFTKLRVLDLDIKIDRTE
                     AFNLFIKKFQSVSLLEEYLRSTPYVMDQLKEAKIDELDLHRAIVALSEKMKAVDDNAS
                     KKKDESALYTSWTLSFTAPTSEEAQKVLAGYIDYISALVVKESIENVRNKLEIKTQFE
                     KEKLAQDRIKTKNQLDANIQRLNYSLDIANAAGIKKPVYSNGQAVKDDPDFSISLGAD
                     GIERKLEIEKAVTDVAELNGELRNRQYLVEQLTKTNVNDVNFTPFKYQLRPSLPVKKD
                     GPGKAIIVILSALIGGIVACGGVLLRHAMASRKQDAIMADHLV"
BASE COUNT         4317 a         5162 c         4703 g         4089 t
ORIGIN      
        1 gtgtgaatat aaaataatgt gctctatctt gcttcgttta tcctataatt agcggattca
       61 tctcacgagt caccccgtaa cgttcagaga gcgtttttag atgtctattt ataaaatccc
      121 gcttccactg aatattctgg aagctgcaaa agaacgtatt acctggacac ttaataccct
      181 accccgtatc tgtgtttctt tttcgggtgg taaagattcc ggattaatgc tgcatctgac
      241 agccgaaatt gcccgacaaa tgggcaaaaa aatctgcgtt ttgtttatcg actgggaagc
      301 gcagttctca tgcactatta actatgttca gtcattacgt gagttttacg ccgatgtcat
      361 cgaagagttt tactgggttg cgctcccgct tacgacgcaa aattcccttt ctcaatacca
      421 acccgaatgg cagtgctggg aacctgatgt cgaatgggtg cgtcagcccc cgcaagatgc
      481 gataaccgat cctgactttt tctcctttta ccagcctggc atgaccttcg aacaatttgt
      541 gcgtgagttt gctgaatggt tttcacaaaa acgtccggcg gcgatgatga tcggcatccg
      601 tgcagatgag tcctacaacc gttttgtcgc catcgccagt ttaaataaac aacgttttgc
      661 cgatgatatg ccctggacca ccgccgcacc aggcggtcat agctggtaca tttaccccat
      721 ctacgactgg aaagtggctg atatctggac ctggtatgct aaccatcaac agctctgtaa
      781 cccactgtat aatataatgt atcaggcagg cgttcctctg cgtcatatgc gaatttgcga
      841 accttttggc ccggagcaac ggcaaggatt atggctctat cacgttatcg aaccggatcg
      901 ctgggctgct atgtgcgcac gagtcagcgg ggtaaaaagt ggcggcattt acgccggaca
      961 tgataatcat ttttatggtc atcggaaaat cctcaagcca gaacatttag actggcaaga
     1021 atatgcatta ttgctgctca atagcatgcc ggaaaaaaca gctgagcatt accgcaataa
     1081 aatcgccatt tatttgcact ggtatcagaa aaaaggcatc gaagttccac aaacccagca
     1141 aggggacatt ggcgcgaaag atatcccctc ctggcggcga atatgcaaag ttttactcaa
     1201 taacgattac tggtgccggg cattatcatt tagtcctacg aaagcgaaga actatcagcg
     1261 ttataacgaa cggataaaag gaaaacgtca ggaatggggg atactatgca acaacgatta
     1321 acgcaggatt taacccaatt tctcgccagc ttgccggaag atgatcgcat caaagcgatt
     1381 aatgaaatcc gcatggcgat ccatcaggtt agcccctttc gtgaagagcc agtggattgc
     1441 gttctgtggg ttaaaaacag ccaacttata cctaatgatt acaacccaaa caacgtggca
     1501 ccacccgaaa aaaaactgct gaaaaaatcc attgaaacag atggttttac ccaacccatt
     1561 gtggtcacgc atacggataa aaatgccctg gaaatcgtgg atggttttca ccgccatgaa
     1621 attggtaaag gctcatcatc gttaaaatta cgcttaaaag gttatttgcc ggtgacctgt
     1681 ctggaaggta cgcgtaatca acgcatcgcc gcgactattc gtcataaccg cgctcgtggt
     1741 cgccaccaaa tcactgccat gtccgaaatt gttcgtgaac tcagccagtt gggatgggac
     1801 gataataaaa ttggcaaaga gctgggaatg gacagcgacg aagtgctgcg cctgaaacaa
     1861 attaacggcc tgcaagagtt gtttgccgac cgtcaatatt cccgtgcctg gactgtcaaa
     1921 taactacagc tggcgcaggc gttcagctgc tgccagcaac gtcgattcct tcttggcaaa
     1981 acagagacga atcagtttat gggggaaggg atcggcgcaa aataccgaca gcgggatcgc
     2041 cgctacaccg tgctcccgcg tcagccactg gcaaaactta acgtcatcta gcgaagaaac
     2101 cgcgctgtaa tccaccagca aaaagtatgt accttcgcac ggtaaaatct ccagccgact
     2161 ctcacttaag gcattcacca gaatatcgcg tttctggcga taaaagtccg gtaacgcaag
     2221 ataatgctca ggttctgcac gtagcatatc ggcaagcgcc aactgtgccg gggtattcac
     2281 cgaaaaggtc agatactgat gcactttgcg gatctcggcg ctgatgggcg caggcgcaac
     2341 gcaataaccc actttccagc cggtcatatg ataggtcttg ccaaacgatg acaccgccac
     2401 cgctcgctca cgcaattgcg gatgcgccag cacgctggca tggccctgtt gtgaaaaatt
     2461 gatgtgctcg tagacttcat cgctaatgac aaaaatctcg tgcccggcga tcgcctgcca
     2521 caaagcggcg aaatcagcct gctgccagac agttgcactg gggttatgcg gggtgttgag
     2581 gatcaccagt cgggtgcgct cgcttaacaa tgcggcaaat tcctgccagt ccacgcgaaa
     2641 atgcggtggt tgcagtgcaa tacgtttcac tattcccccc gaaagcgcga tggcgggcgc
     2701 gtaactgtca tagctgggat caaagcagat cacttcatca ccattgcgca ccaatgcggt
     2761 aatcgctgca tataacgctt ccgtcgcccc tgccgttacg gtgatatcgc tatcggcatc
     2821 cggttgatag ccatacagac gttccgtttt ctgagcaatc gcctcgcgca aggcctgcac
     2881 gccggtcatg ggcgcgtatt ggtttgcccc ctgggcaacg tggtacgcca gccgctcctg
     2941 taaatagcgc ggaccatcaa aatcaggaaa gccttgtgaa aggttaatcg cctggtgttg
     3001 ctgcgccagc gcgctcatcc gggtgaaaat agtggtgcca agttgtggaa gtttgctttg
     3061 tggaatcaga gggttatttg tcatttttat tgtacctgac gaagttgcgt gttgaaggca
     3121 ctataacacg atgctagtat ttggcaatca agacgtttag atgtctaaat aaaacaataa
     3181 ggacaacaca acatgcctca caatcctatc cgcgtggtcg tcggcccggc taactacttt
     3241 tcacatcccg gaagtttcaa tcacctgcac gattttttca ctgatgaaca actttctcgc
     3301 gcggtgtgga tctacggcga acgcgccatt gctgcggcac aaaccaaact gccgcctgcg
     3361 tttgaactgc caggggtaaa gcatattttg tttcgtggtc attgcagcga aagcgatgtg
     3421 caacaactgg cggcggagtc cggtgacgat cgcagcgtgg tgattggcgt cggcggcggc
     3481 gcactactcg acaccgcgaa agccctcgcc cgccgtctcg gtctgccgtt tgttgccgtt
     3541 ccgacgatcg ccgccacctg cgccgcctgg acaccgctct ccgtctggta taacgatgcc
     3601 ggacaggcgc tgcattatga gattttcgac gacgccaatt ttatggtgct ggtggagcca
     3661 gagattatcc tcaatgcgcc gcaagaatat ctgctggcgg ggatcggtga cacgctggcg
     3721 aaatggtatg aagcggtggt gctggctccg caaccagaaa cgttgccgct aaccgtgcgg
     3781 ctggggatca ataatgcgca ggccattcgc gacgtcttgt taaacagtag cgaacaggcg
     3841 ctgagcgatc agcaaaaaca acagttaacg caatcatttt gcgatgtggt ggatgctatt
     3901 attgctggtg gtgggatggt cggtggtctg ggcgatcgtt ttacgcgtgt ggcggcggcc
     3961 catgccgtgc ataacggtct gaccgttttg ccgcaaaccg agaagtttct ccacggcacc
     4021 aaagtcgcct acggaattct ggtgcaaagc gccttgctgg gtcaggatga tgtgctggcg
     4081 caattaacag aggcgtatca gcgttttcat ctgccgacta cgctggcgga gctggaagtg
     4141 gatatcaata atcaggtgga gatcgacaaa atgattgccc acaccctacg tccggtggaa
     4201 tccattcatt acctgccagt cacgctgaca ccagatacgt tgcgtgcagc gttcgaaaac
     4261 gtggaatcgt ttaaagcctg acgtacaaat ttagcagcag cgcgcgccgc cttttccacc
     4321 gtagcgcgcg tcctgccgct cccggaaaaa ctcctcgtag gtcatcggtg tttgatcggg
     4381 atggttaacc cgcatatgtt cgacatagtt gtcgtaatca ggcataccaa tcatcagttt
     4441 cgccgcctga cctaaatatt ttccggcttt tgccagtgaa tcaaacatct aaacctccgg
     4501 taatacaaat gccctcctga attaagaggg cttaattgct atcaacacgc tcgggatgag
     4561 agtaagggga acgctcagca cattccccct cactctgact ttagtgcgcg cctttcgcct
     4621 gcgccacgat ctcctcgaca ttttccggca tcggctcata tggcgtttct ttcgccgttg
     4681 gcttcggctc tttcaatgcc accagtgccg tcttaatcga gaacagcgcc agaaccacca
     4741 caaccaccat aaagaagatg gttaatccgg catccagacg gttgttgaac accagttgcg
     4801 ccagttgtga ctcagtatac tgcgacggaa tattgccgct gtcgatcatt gcctggaact
     4861 tgttagcaat cgccaggaag ccgactttcg catccgggct aaacgctttc tgccagcctg
     4921 cggtcagggt acaaatcagc agccaggccg ttggtaccag cgccacccag gcgtaacgtt
     4981 gacgtttcat tttgaacagc accacagcac agagcatcag cgccatccct gccagcatct
     5041 ggttggcaat accaaacagc ggccacaagg tgttaatacc gcccagcgga tcgaccacgc
     5101 cctgatgcag gaagtagccc cacgccagca cgcacagcgc cgttgccagc aggttagcag
     5161 gcagtgaatc ggtccgtttc aggcccggag acaccacgcc cagcagatcc tgcaacataa
     5221 agcgcgcagc acgcgtaccc gcatccaccg ccgtcagaat aaacagtgct tcaaacagaa
     5281 tggcgaagtg ataccagaac gccacatcca tcatgccgcc cagcgcaccg tgcagaatgt
     5341 aggccatccc caccgccagg gtcggcgcac cgcccgcacg agaaatgatc gactgttcac
     5401 ccacttcgct ggcaatctgg tttagcgtat ccggggtaat cgcaaagccc cagctactca
     5461 ccacctgcgc ggcagaagca accacatccg ccgtccctgc cggagccagc accgccatcg
     5521 ggctgttcat ggcgaagtac acacccggat cgatgataca ggcggaaacc agcgccataa
     5581 tcgccacgaa ggattccatc aacatcccac cgtagccgat aaagcacgcc tgcccttcgt
     5641 tcgccagcat cttcggtgta gtgccggaag agatcagtgc atggaagcca gacaccgcgc
     5701 cacaggcgat ggtgataaac aggaacggga acaggttacc ggtccatacc gggccagtgc
     5761 catcgacaaa tttagttagc gcaggcatgg tcagcgtcgg gcgcataatc aaaatgccca
     5821 ccgccagacc gacgatagtc ccgattttca ggaaggttga gaggtagtca cgcggagcca
     5881 gcagcaacca caccggcagc accgccgcca caaaaccgta acccaccagc atccaggtca
     5941 gctgcacgcc ggtaaagtca aagtacggtg cccacgtcgg gctttctgcc acccagccgc
     6001 cagagataat ggcgaaaata aggaacacca gaccaatcac cgacacttcg ccaatacgcc
     6061 ccggacgcaa atagcgcagg tagatcccca taaacagcgc cagcggaatg gtgaacgcaa
     6121 cggtatacgt tccccacggg ctatgagtca gggctttcac cacgatcatc gccagcacgg
     6181 caaggataat gaccatgatc ataaagcagg ccaccagcgc aatcaccccg gcggttggcc
     6241 ccatctcttc tttgaccaac tcacccagcg agcgaccatc gcggcgcgta gaaacaaaca
     6301 gcaccatgaa atcctgcacc gcaccggcga gaaccacccc agcgagcagc cagatcatcc
     6361 ctggcaggta acccatttgc gccgccagta ccggcccaac caacggacct gctccggcaa
     6421 tggccgcaaa atggtgaccg aacagcactt ttttgtccgt cggcacatag tccagcccgt
     6481 cgttatggcg caccgctggc gtcatacgcg tcgggtcaac cgccagcaca tttttggcga
     6541 tatacagccc ataaaaacgg taagcgatca gatagataca gaccgacgcc accacaatcc
     6601 acagcgcgtt gatctgttcc ccacgattta aagcaatgta tcccagagca aatgctccca
     6661 ttacagagag cactgtccag acgaggtatt tccctgattt gttcatagtt gttatccgtg
     6721 tgcgtgtcca tagagatgtt acattttgtt tctataacat ctttgtacaa tttaacaaca
     6781 cggcaaccat gtaaatcgga gttgaaacca atatttaact taacaatgtt aactcgatca
     6841 ctccgttaac cgagttttcc tgcaatctca tttaattctg tcgggctcat cccaaaactg
     6901 ccgtacccag ccgacaagtg cagcaacgcc gcccctgttc atcgaaaaca acaatttccc
     6961 agctctgatt ttgccgacca agatgcagcg gctggcagac gccgcgtacc tttccctcag
     7021 acaccgggcg atgatgtgtg gcgttaagtt ccgtgcccac cacacactgt ccgtcgcgag
     7081 tcatcataaa tccggccatc gatcccagcg tttccgccag cgccgccgac gcgccgccat
     7141 gtagtaaacc gaacggctga tgagtacggg tatcaaccgg catttcggct tccagcacat
     7201 catcgcccag gcgggtatac acaattccca gatgcgccac cattgtgtta tcgctggtgg
     7261 cgttcagctc gtcgagcgtt aaatggcgtt tccagatcat gcttatgccc ccagcgtaga
     7321 gccgccatct accacaatat cctgtagggt gatatggctg gcgaggtcag aggcgaggaa
     7381 caaaatcgtg ttggcgatct cttgtggacg ggcgattttc cccagcggaa tgccaagttt
     7441 aaactgctcg ccaaagccgc gaatacgctg ctcttcggcg tcatcgctca cccacagcgt
     7501 gcgttgcata tcggtgtcgg tggagcctgg cgaaaccaca ttacagcgca cgccgctacc
     7561 cgccagttcc agcccaacgc tcaacgccag gcttttcagc gccgctttcg acgcgccata
     7621 agcactcata ccaatgcgcg gcgtgtgtgc ggcgtcggac gcaacagtaa caatcgcccc
     7681 gccccgctga cggcgaaact ggttcatggt ctgctggaac aggttaaacg caccgccgac
     7741 attaaccgca aaagtctgct gccagtcctc cttactgagc tgatcggtcg cgcccatgcg
     7801 caaaattccc gccgcattga ccagcacgtc cagtcgctcc gtttcagcta acagtcgctg
     7861 acatacctgc gccacctgcg cagcgtcggc aacatccatc acttcggtcg caaagggata
     7921 ttgctcctga gtgaacgctt gatcaaaacc tgtaactttc gctcccgcct caacaaacgc
     7981 cagcgccgtg gcgtagccga tacctttccc cgcgccagtt acccagacat ttttaccgct
     8041 gaaatccatt atttcacctc gcgggagagt agcttccacc aggcgtcgat ggtcgggttt
     8101 ttcgccagca tgacaaagtc gatatcgcca tgcactttgc gccagcgcgc cgccagcgcc
     8161 atcatccgca ccgaatccag accgtagtcg atcaggttgt cgtcatcgaa cggttcatcg
     8221 gactcgtcca gcaacggcag gatcacctca cgcagcgccg ctttgctggc ggggataggt
     8281 gctggcagta attcttcggt catcaccact cggccagaac gtccggccac atatttcagc
     8341 gacatcaaat gctcgtcacg gctgaaatcg gccagcgcat ctgccaccat aaacggttta
     8401 atatcgcgca taaatgcgtc ggttgcggtg gtcatacagc caatgtgggc atataccccg
     8461 gtaataatca gctggttacg tccactctct ttcagcattt gctccagcgg ggaacgatga
     8521 aacgcgctgt agcgccactt caccagcacc gtgtcgtcgg catctggcgt caggctatcc
     8581 accacctttt gctgttccgg cgaacgggtc aggccaggcc cccacatatc attcaacagc
     8641 gcccgatctt catcgctctg ctctttcggc tgggcggtgt aataaaccgg gatattgtgc
     8701 tgtttgcagt agtcgcgtag tgccgcaata ttcgcgatca cctgctccat catcgggcag
     8761 ttctcgcccc agaagctgac aaaatagtcc tgcatatcat ggattaacaa cgcggcacgt
     8821 tgcggttcaa aggcccagtc gactttattc tgcggaatat cgtgagactc cggcagtgcg
     8881 taagcctgta attttggaat agccatcgtg ttctctcctt caggctgatg cgcgtgacgc
     8941 cagccactga cgtaattgtt ttttatcgac tttcccgacc gccgtcagcg gaagtgaatc
     9001 tacacactcc acgcgatccg gtaatttaaa ttcggcaata ccctgttcac gcaggaaacg
     9061 acgcacctgc accgcgcgca gcggctcttt taccaccaga taagcacagc ttttttcgcc
     9121 catcagctca tcttccatgc tcaccagtgc ggcgtagatc accgccggat ggcgcagcag
     9181 caggttttcg atctcttcgg cagcgatctt ctcgccgcca cggttgatct gatctttctc
     9241 gcgcccctgc acagtgatgt aaccctctgg atcaatagag atcagatcgc cggaacagta
     9301 aaaaccgttg gcatcaaagg cgctggcatt gtgctgcggg cttttgtaat agccacggaa
     9361 ggtatacgga ccgcgggtca tcaggcgtcc aacttcccct tgcggcagtg gatttccttc
     9421 ggcatcggca acccacactt catcgtccgg acacattggg taaccctggg tatggataat
     9481 tttctccgcg ctatcatcaa gtcgggtgta gttcactagt ccttccgcca tgccgaatac
     9541 ctgctgtaac tggcagccaa tctctgcggg aatacgcgcc gcaagcgtgg cagaaagacg
     9601 tgcaccaccg acctgtaaca gtttcagcga ggcaagctgc gcccggcttt cgccttcagc
     9661 cagtgcctgc aaccacaggc tgactgcggg cggcaccagc gcggtaacgt tgacctgatg
     9721 tttttcaatc aatgggaagc aaagcgtggc gctggggtcg gcagccagaa cgacagtgcc
     9781 gccagcgaga aataccccca acgatcccgg cgaactcatg gcgtagttat gagccgccgg
     9841 gatcgcgcac aggtagcgcg tctgttgtgt gaactgacaa atctcgacgc tacgacgcac
     9901 gctgtagtag tagtcgttat gagtgcgcgg tatcagtttc ggagtgccgg tggtgccgcc
     9961 ggaaagctgg aaataggcca cttcatcagc aggagatggc gtggcagtaa aatcctcagc
    10021 cggatggtta atcgcatcct gcaagttatg ctcaccgctg tcgttgagca gctgcaccac
    10081 gcgaatggaa gaatgctctg caacaaatgt gttgaggaaa tcatccccgc taaatagcgc
    10141 atgttgacga tcggcaatca gtaacgccgg ttcaatctgg ctggcatagg cgttcagttc
    10201 actacgctga tggctgaaca gcgccagcac cggcgcaacg cccagcttca gcagcgcgaa
    10261 aaaggtaata tacaattcag cgacgttacc cagttgtacc agcgcggttt caccaggttt
    10321 aatgccctga cggcgcaaac tacaggcgag gttatccgcc gcctgattca gctcccggta
    10381 actcaactgc cgctcgccgt cgataaccgc gatgctgtca ctcgcagcgt ggcgagtcag
    10441 aatgtcggtc agtggcaaat cctgccagta gcctttttcc cgatagcgac gggcaaactc
    10501 ttccggccag cgggtgaatg gaatgctcat cctcgctcct taatgcaatc caaaaacgtt
    10561 caacatggta gaaagtttga cgcctgtttc gcgccactca cccaacggtg aggacgcagg
    10621 cacaatcccc gctccggcaa acagacgcac ctgattttcc cgcagcttcg cgcagcggat
    10681 ggtcaccacc cattcaccgt taccttcgct gtcacaccaa cccacaatgc cgccaaacag
    10741 ttcgcggtcg aacggctcca gttcagcaat aacctgagtc gcggcctgat gcgggaaacc
    10801 gctcagcgcg ggggtcggat gcagcagaca ggccagagtc agtgcgtttt cttgcgaatt
    10861 cgctttacct tcaaagggag ttgcgagatg ccacagcgtc ggcgtggtaa tcaattgtgg
    10921 agaggaggga acgtgtaact cactactgcg tttgcgcagt acctctttca tcgcctgagt
    10981 caccagttca tgttcatggc gatctttttc tgacgccagc agacgattac ccgcttcgcg
    11041 atcgagcact tcatccggct gacgacgcgc ggaaccggct aacggaatgg agctaaaacg
    11101 ctcgccgtct ttacgtagca gcagttccgg gctggccccc agcaggacgc cgccatcagc
    11161 cagcgggaca tggaagttgt aactaaccgg gttttgcgca atcaaccgtt ccagcaatac
    11221 gccactatca atggcggcgt cagtggtgat atcaatcaac cgtgacaaca ccactttgtc
    11281 gacctgcggc gtggcggtaa gtgcggcggc gcgggcaacc atctgttcaa acgtggtttg
    11341 ttccggaatt gcctggcgtt ccaccacatt cagcgactgg ctgcgggtga aacggcgtgc
    11401 ggaagcttgt ttttcctgac gggagaacga ctgccaggat tcgggaatat acagcgatga
    11461 aggctgacgt ggatcgaagg gaatcgcccc gaccatcacc ggatttttga tgccctgcgc
    11521 tttggcaccg gcaaacagcg cggcgagttt ttgctggaag ggactgtcgg gcgaatcccc
    11581 gttcacagcc ggttcatcga agcgggcgaa acatcctgac gtcgtaaaac tgcggtacgg
    11641 cgacataaag aaaaagcgat tgggcgcaag tgttgccatg gtctgctgta cttcctcagc
    11701 cagtgacgta tccatatcat cctccacaaa atgataaagg ctttaataat gattatcatt
    11761 tatattttcg gtcgctaacc taaaagcaaa cgcgcactat gtcaactctt gaggtaacgc
    11821 aatctgcaac tcggggttgc atctggttgt ggttacaatg aaaatgagaa gcattaactt
    11881 cattaataac aggaagctga tttgtgagac tcgtcccgct ctaccgcaac gcccttctat
    11941 taacaggact tttgctttca ggaatagccg tagttcaggc cgccgactgg ccgcgtcaga
    12001 ttactgacag ccggggcact cataccctgg aaagccagcc gcagcgtatt gtttccacca
    12061 gcgtcaccct gactggctca ctgctggcga ttgatgctcc ggtgatcgcc agcggcgcga
    12121 ccacgccgaa taaccgcgtc gcggatgacc agggcttttt acgccagtgg agcaaggtgg
    12181 cgaaagaacg caaactgcaa cggctctata tcggcgagcc gagtgcagaa accgttgccg
    12241 cgcaaatgcc ggatctgatt ttaattagcg caaccggcgg tgattcagcg ctggcgctgt
    12301 acgatcagct ttccaccatc gccccaacat taatcatcaa ttacgacgac aaaagctggc
    12361 agtcgctgtt aacgcagctt ggcgaaatta ccgggcatga gaaacaagcg gcagagcgga
    12421 ttgcacagtt tgataagcaa ctggcggcgg cgaaagagca aatcaaatta ccgccgcagc
    12481 cggtcactgc cattgtctat accgccgctg cacacagtgc caatctctgg acgccggaat
    12541 cagcacaagg gcagatgctg gaacaactcg gctttacgtt ggcgaaattg cccgcagggt
    12601 taaacgccag ccaaagccag ggcaaacgcc atgacatcat tcagcttggt ggggaaaatc
    12661 tggcttcagg gttaaatggc gagtcactat tcctgttcgc cggtgatcag aaagatgccg
    12721 atgcgattta tgctaatccg ctgctcgcgc acctgcctgc agtacaaaac aagcaggttt
    12781 atgcgctggg aaccgagacg ttccgtctgg attactacag cgccatgcaa gtgctggaac
    12841 ggcttaaggc gctgttttaa gcattaactg tcggacgctg tcacctgcgg cggcgtctgg
    12901 cgaaaacgcc gcaactccac cagcaccagc aataacaaca cgccgataat caacaaacca
    12961 aaaccgctcg cgcttgcgga agcaaccggt gtcatcatcg cgcccaaacc gcccagcagc
    13021 gccgcgccta tggcatcgcc cgtcacgttt tgcgccgtcc acaaaccgtt aatccgccct
    13081 aacatcgctt ccggggtttg cgtttgcagc attgtgtatt gcagcaacga actaaccgcg
    13141 ctcagccagc cgaacagcgc cagacaaacc acgcctaaaa tccacatcgg catcaggcca
    13201 aacagaccaa tggcgaggaa cgatcccagc gtggaaagca gcatcaataa ccctggtcgc
    13261 gcgctatgtg ccagcttccc gctggttaac gcgccaatag ccgcaccgag cgggatcgcc
    13321 gcatagagaa aaccaatctg tgccgccgac atctgccagt tgtcagccag cgccggatac
    13381 aggacccgca ccgcgctcgc catcgtcaat aaaccaccca acagcgcaat ccctcctacc
    13441 agcgggctgg cgagcagaaa acgaaatcct gccagtaatg atttcaacgg atgctcacgc
    13501 ggctgcggtg gtggtggtaa cgccggaagg cttaacaacg gtagcaaggt aataaacgtg
    13561 cccgccgccg ccagcccgta gttccaggcc acaccgccag tcgccagcag taaaccgcca
    13621 atcatgggcg aaatcaccga tcccagacgc acggtcaaca tggtgatcgc cccggcctgc
    13681 attaagtttt cacgccctac cagtgctggt gtcgccgcca gtagcgccgt aacgccaagt
    13741 gatgcgaaaa aaccatccca taaaccaagt aaatagattg ccagcaatga cggctccggc
    13801 agcagtgcat taaggcacag tccaatgaag ccaatgccac aggtgccgcg cgccagcaaa
    13861 atcacttttt tgcgctcata gcgatccgcc agcacaccgc cgaccatcag gccaacaaac
    13921 atcgcgccgc cggtcagcgt caccgaaagc cccacctgcc aggtagaatg cgtcattatc
    13981 tggatctgca ccggtaccgc gacgccgagc aaacccagag acacaattga gataaaacga
    14041 gcgaggaata ctgcgcgaaa cgccgggtgc gttttcaaca ggctgaggtt aagcagccag
    14101 gattgtttat tcattacaat gccttgccat caaatatttt aaatacctat tcttaggctg
    14161 cacatgctaa catatccaaa taagatcgat aacgataatt aatttcatta tcatggaagt
    14221 tcgtatgtct ggttctgttg ccgtgacacg cgccattgcc gtgcccggat tgctgttatt
    14281 actgattatc gcgacggcat taagcctgct cattggggca aaatcactcc ccgcttccgt
    14341 agtgctggag gccctctccg gcacctgcca gagcgccgac tgcaccatcg tgctcgacgc
    14401 gcggctgccg cgcacccttg ccggtttact ggcaggcggc gcgcttgggc ttgccggggc
    14461 gttaatgcaa accctcaccc gaaacccact tgccgacccc ggcttgcttg gcgtaaacgc
    14521 cggagccagc tttgccattg tgctgggtgc ggcgctgttt ggttactcat ccgcgcagga
    14581 acaactggcg atggccttcg ccggggcgct ggtggcctcg ttgattgttg cctttaccgg
    14641 cagccagggc ggcgggcagt taagtccggt gcgtttaacc ctggcgggcg tggcgctggc
    14701 ggcggtgctg gaagggctga ccagcggcat cgccctgctt aatcctgacg tctacgatca
    14761 actgcgtttc tggcaagccg gttcgctgga tattcgcaat ctatatacct taaaagttgt
    14821 gctgatcccg gtgctgatcg ccggagcaac cgcgctatta ctgagccgcg cgctgaacag
    14881 tttaagcctc ggcagtgaca ccgcgacagc gctgggcagc cgcgtggcgc gcacacagtt
    14941 gattggtctg ctggcgatta ccgtgctttg tggtagtgcg acggcggtag ttggcccgat
    15001 tgcctttatt ggcctgatga tgccgcatat ggcgcgttgg ttggttggag cagatcatcg
    15061 ctggtcgctg cccgtcacgc tacttgccac ccctgccctg ctgctgtttg ccgatatcat
    15121 cgggcgggtg attgtgcccg gcgaactgcg agtttccgtg gtcagtgcgt ttattggtgc
    15181 accggtgctg atcttcctcg tgcgacgtaa aacgcgaggt ggcgcatgat ttacgtctct
    15241 cgccgattaa ttatcacctg tttgctgctg gtttccgcct gtgtggttgc aggtatctgg
    15301 ggattacgca gcggtgccat cacgctggaa acctcgcagg tattcgccgc gctgatgggc
    15361 gatgcgccgc gcagtatgac gatggtggtc accgaatggc gtttaccacg cgtgctgatg
    15421 gcgctgttga ttggcgcggc gctgggcgtc agcggcgcga tttttcagtc gctgatgcgt
    15481 aacccgctcg gcagccctga cgtaatgggc tttaacaccg gggcatggag cggcgtactg
    15541 gtggcgatgg tgctgtttgg tcaggatctg acggctatcg cgctggcagc aatggcgggg
    15601 ggtattgtca cctcgctgtt ggtctggctg ctcgcctggc gtaacggtat cgacaccttt
    15661 cggttgatta ttatcggtat cggtgttcgc gccatgctgg tggcctttaa tacctggctg
    15721 ttgctgaaag cgtctttaga aacggcgcta acagcgggtt tgtggaatgc cggatcgctc
    15781 aacggcctga cgtgggcaaa aacctcacct tccgcgccaa tcattatatt gatgctcatt
    15841 gccgcctcct tgctggtgcg gcgaatgcgc ttgctggaaa tgggcgatga taccgcatgt
    15901 gcgctgggcg tcagcgtcga acgttcgcgg ctgttaatga tgctggtcgc cgtagtgctt
    15961 accgctgcgg caacagcgct tgccgggccg atttccttta ttgctttagt cgcgccgcac
    16021 attgcccgcc gcattagtgg caccgctcgc tgggggctaa cccaggcggc gctgtgcggg
    16081 gcgttgttac tgctggtggc cgatctctgc gcccaactac tgtttatgcc gtatcaactt
    16141 ccggttggcg tcgttaccgt cagcctcggt ggtatttacc ttatcgtctt gttaattcag
    16201 gagtctcgca aaaaatgacc gaatcagtag cccgtttgcg cggcgaacag ttaaccctcg
    16261 gatatggcaa atataccgtt gcggaaaatc tgactgtaga aatacctgat ggtcacttca
    16321 cggcaattat cgggccaaat ggctgcggta aatccacgtt gctgcgtacc ttaagccgcc
    16381 tgatgacgcc tgctcacggg catgtctggc tggatggcga gcacattcaa cattacgcca
    16441 gtaaagaggt tgcacgccgg attggcctgt tggcgcaaaa cgccaccacg ccgggagata
    16501 tcaccgtgca ggagctggtg gcgcgtgggc gttacccgca tcaaccgctg tttacccgct
    16561 ggcgcaaaga agatgaagac gcggtaacga aagcgatgca ggccacggga atcactcatc
    16621 tggcagatca aagcgtggat accctttctg gcggacaacg ccagcgagcg tggatagcaa
    16681 tggtgctggc ccaggaaacg gcaattatgc tgctcgatga acccacgacc tggctggata
    16741 tcagtcatca gattgatttg ctggaactgt taagcgaact gaaccgcgag aaaggctata
    16801 ccctggcggc ggtgctgcac gatcttaatc aggcctgtcg ttacgccagc catttgattg
    16861 cattgcggga agggaaaatt gttgctcagg gagcgccgaa ggagattgtc actgctgaac
    16921 tgatcgagcg catttatggt ctgcgctgca tgattattga cgatccggtt gccggaacgc
    16981 cgctcgtggt gccgctcgga cgaacgacac cttcaaccgc gaagatttaa actaagtggt
    17041 ctgccattat ggcatcctgt tttctggatg ccatcgcatg gcgcaatagc acgccaccac
    17101 aagccactat accgccgatc aacgcagaaa ggatcacaat aatcgcctta cccggaccgt
    17161 ctttttttac gggtaatgac ggacgcaact gatatttaaa cggtgtaaaa ttcacatcgt
    17221 tgacatttgt ttttgttaat tgctcgacta gatactgacg attacgtaat tcaccgttca
    17281 attccgcaac gtcagtgact gctttttcaa tttccagttt gcgttcaata ccgtctgcgc
    17341 cgagagaaat agagaaatcg gggtcatctt taacggcctg accattactg tatacgggct
    17401 ttttaattcc tgccgcgttg gcaatgtcga gtgaataatt gaggcgctga atgtttgcat
    17461 caagttgatt tttcgtttta atgcgatcct gagccagttt ttctttttca aactgggttt
    17521 tgatctccag tttattacgg acgttttcta tcgactcttt caccaccaac gcagagatat
    17581 aatcgatata cccggccaac actttctgtg cctcttcact ggtgggtgcg gtgaaactta
    17641 gcgtccagga agtatacaat gcagactcat cttttttctt actggcattg tcatcaaccg
    17701 ctttcatttt ttcgctcaat gcgacaattg cgcgatgcaa atccagttcg tcgattttcg
    17761 cctcttttaa ttggtccatc acataaggtg ttgagcgcag gtactcttcc agcaagctaa
    17821 ccgactgaaa cttcttgata aacaggttaa atgcttctgt acgatcaatt ttgatatcca
    17881 gatccagcac acgaagctta gtgaatgttt tctcaagcgc ctgccactga acaggttctg
    17941 gaggcgtgac aaccgccgcg ctggtccatt tttgcggcag gatgaaagag atcagtaaac
    18001 ctgcgcaggc aaacgcaaaa acgaccgcca tgaccgtttt tttggcccgc cataaaactt
    18061 caattagatt aagtaaatca acttcattat tactgggcga cgccagagga tactcgggaa
    18121 aatgagcttc acttccctgt ttaatattca gtgatgacat ggggtataac ctgaaaatga
    18181 gtccaataag agtaaatctc cacggccccc actctaccag ttggtgagat ttatccgaag
    18241 ctgaagtttg taaattactc ccactgaatg c
//
LOCUS       DAZFLD010000092        18250 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.092, whole genome shotgun sequence.
ACCESSION   DAZFLD010000092 DAZFLD010000000
VERSION     DAZFLD010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18250
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.092"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..564)
                     /locus_tag="ACHKBL_002652"
     CDS             complement(<1..564)
                     /locus_tag="ACHKBL_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5819505.1"
                     /translation="MSIDFTPCMINTYHGDIYNRTTDTDNVKTPDTPKWPCDNWEEQQ
                     PINSTFSGEGYTSDQYDLAQYQLQQINACHTNTTYTNADYSKVVAQLVSLITNIETIS
                     STQLTQQTQSILNQINNIRYEKNKSAEYRIIVVANPKPDKAIITKISVEEGIPVRFSV
                     QTMFSDTNFITEQRADLPTNIKDIQSLY"
     gene            complement(1145..2146)
                     /locus_tag="ACHKBL_002653"
     CDS             complement(1145..2146)
                     /locus_tag="ACHKBL_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFO5819506.1"
                     /translation="MSSWLAMLQDATEMFVFLAVELSLLFIVISAGVSLIRQKVPDHK
                     IQQMMGARKGKGYLLAALLGAVTPFCSCSTIPMLRGLLSAKAGFGPTLTFLFVSPLLN
                     PIIVGLMWVTFGWKVTLLYAIIAAGVSVLASIILDSLGFERHIIASKSSSANCCAPAK
                     TSPGTTYTPIKVSCCSPAAKAIEKPVVNCCSTKAVVSINPIKLATKDALQQFKDVLPY
                     LLLGVLIGSFIYGFIPSEWIAAHAGADNPFAIPLSAVVGIPLYIRAEAVIPLASVLMT
                     KGMGLGALMALIIGSAGASLTEVILLKSMFRIPMIVAFLTVILGMAILMGYLTQMLF"
     gene            2253..2549
                     /locus_tag="ACHKBL_002654"
     CDS             2253..2549
                     /locus_tag="ACHKBL_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFO5819507.1"
                     /translation="MQLEEVAKALKELGHPTRLFIFKHLVKAGEQGLPVGELQKQLGI
                     PGSTLSHHISALVSVGLVKQNRESRTLMCVSQYAMLEAIIEFLREECCVNSKIA"
     gene            complement(2672..3097)
                     /gene="arsC"
                     /locus_tag="ACHKBL_002655"
     CDS             complement(2672..3097)
                     /gene="arsC"
                     /locus_tag="ACHKBL_002655"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-dependent arsenate reductase"
                     /protein_id="HFO5819508.1"
                     /translation="MSNITIYHNPACGTSRNTLEMIRNSGTEPTIVYYLETPPTRDEL
                     VKLIADMGISVRALLRKNVEPYEELGLAEDKFTDDRLIDFMLQHPILINRPIVVTPLG
                     TRLCRPSEVVLEILPDAQQGAFTKEDGEKVVDEAGKRLK"
     gene            complement(3110..4399)
                     /gene="arsB"
                     /locus_tag="ACHKBL_002656"
     CDS             complement(3110..4399)
                     /gene="arsB"
                     /locus_tag="ACHKBL_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite/antimonite:H(+) antiporter ArsB"
                     /protein_id="HFO5819509.1"
                     /translation="MLLAGAIFVLTIVLVIWQPKGLGIGWSATLGAVLALVTGVVHPG
                     DIPVVWNIVWNATAAFIAVIIISLLLDESGFFEWAALHVSRWGNGRGRLLFTWIVLLG
                     AAVAALFANDGAALILTPIVIAMLLALGFSKGTTLAFVMAAGFIADTASLPLIVSNLV
                     NIVSADFFGLGFREYASVMVPVDIAAIVATLVILHLYFRKDIPQNYDMALLKSPAEAI
                     KDPATFKTGWVVLLFLLVGFFVLEPLGIPVSAIAAVGALILFVVAKRGHAINTSKVLR
                     GAPWQIVIFSLGMYLVVYGLRNAGLTEYLSGVLNVLADNGLWAATLGTGFLTAFLSSI
                     MNNMPTVLVGALSIDGSTASGVIKEAMVYANVIGCDLGPKITPIGSLATLLWLHVLSQ
                     KNMTISWGYYFRTGIIMTLPVLFVTLTALALRLSFTL"
     gene            complement(4454..4807)
                     /gene="arsR"
                     /locus_tag="ACHKBL_002657"
     CDS             complement(4454..4807)
                     /gene="arsR"
                     /locus_tag="ACHKBL_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="As(III)-sensing metalloregulatory
                     transcriptional repressor ArsR"
                     /protein_id="HFO5819510.1"
                     /translation="MSFLLPIQLFKILADETRLGIVLLLSELGELCVCDLCTALDQSQ
                     PKISRHLSLLRESGLLLDRKQGKWVHYRLSPHIPAWAAKIIDEAWRCEQEKVQVIVRN
                     LARQNCSADSKNICS"
     gene            complement(5055..5270)
                     /locus_tag="ACHKBL_002658"
     CDS             complement(5055..5270)
                     /locus_tag="ACHKBL_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000968019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5819511.1"
                     /translation="MMAISLQSEPPTQGWKVKNRRKVGFLHPDSDKAWYGSDPMKVYK
                     SSFTNMYSKLSLRQLKKSLTLAMLATG"
     gene            5549..5632
                     /gene="dinQ"
                     /locus_tag="ACHKBL_002659"
     CDS             5549..5632
                     /gene="dinQ"
                     /locus_tag="ACHKBL_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible type I toxin DinQ"
                     /protein_id="HFO5819512.1"
                     /translation="MIDKAIIVLGALIALLELIRFLLQLLN"
     gene            complement(5686..7038)
                     /gene="gorA"
                     /locus_tag="ACHKBL_002660"
     CDS             complement(5686..7038)
                     /gene="gorA"
                     /locus_tag="ACHKBL_002660"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HFO5819513.1"
                     /translation="MTKHYDYIAIGGGSGGIASINRAAMYGQKCALIEAKELGGTCVN
                     VGCVPKKVMWHAAQIREAIHMYGPDYGFDTTINKFNWETLIASRTAYIDRIHTSYENV
                     LGKNNVDVIKGFARFVDAKTLEVNGETITADHILIATGGRPSHPDIPGVEYGIDSDGF
                     FALPALPERVAVVGAGYIAVELAGVINGLGAKTHLFVRKHAPLRSFDPMISETLVEVM
                     NAEGPQLHTNAIPKAVVKNADGSLTLELEDGRSETVDCLIWAIGREPANDNINLEAAG
                     VKTNEKGYIVVDKYQNTNVEGIYAVGDNTGAVELTPVAVAAGRRLSERLFNNKPDEHL
                     DYSNIPTVVFSHPPIGTVGLTEPQAREQYGDDQVKVYKSSFTAMYTAVTTHRQPCRMK
                     LVCVGPEEKIVGIHGIGFGMDEMLQGFAVALKMGATKKDFDNTVAIHPTAAEEFVTMR
                     "
     gene            complement(7110..7952)
                     /gene="rlmJ"
                     /locus_tag="ACHKBL_002661"
     CDS             complement(7110..7952)
                     /gene="rlmJ"
                     /locus_tag="ACHKBL_002661"
                     /EC_number="2.1.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase"
                     /protein_id="HFO5819514.1"
                     /translation="MLSYRHSFHAGNHADVLKHTVQSLIIESLKEKDKPFLYLDTHAG
                     AGRYQLSSEHAERTGEYLEGIARIWQQDDLPAELEAYINVVKHFNRSGQLRYYPGSPL
                     IARQLLREQDSLQLTELHPSDYPLLRSEFQKDSRARVEKADGFQQLKAKLPPVSRRGL
                     ILIDPPYEMKTDYQAVVSGIAEGYKRFATGTYALWYPVVLRQQIKRMIHDLEATGIRK
                     ILQIELAVLPDSDRRGMTASGMIVINPPWKLEQQMNNVLPWLHSKLVPAGTGHATASW
                     IVPE"
     gene            8155..10197
                     /gene="prlC"
                     /locus_tag="ACHKBL_002662"
     CDS             8155..10197
                     /gene="prlC"
                     /locus_tag="ACHKBL_002662"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020317218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HFO5819515.1"
                     /translation="MTNPLLTPFELPPFSKILPEHVVPAVTKALNDCRENVERVVAQG
                     APYTWENLCQPLAEVDDVLGRIFSPVSHLNSVKNSPELREAYEQTLPLLSEYSTWVGQ
                     HEGLYKAYRDLRDGDHYATLNTAQKKAVDNALRDFELSGIGLPKEKQQRYGEIATRLS
                     ELGNQYSNNVLDATMGWTKLVTDEAELAGMPESALAAAKAQAEAKELEGYLLTLDIPS
                     YLPVMTYCDNQALREEMYRAYSTRASDQGPNAGKWDNSKVMEEILALRHELAQLLGFE
                     NYAFKSLATKMAENPQQVLDFLTDLAKRARPQGEKELAQLRAFAKAEFGVDELQPWDI
                     AYYSEKQKQHLYSISDEQLRPYFPENKAVNGLFEVVKRIYGITAKERKDVDVWHPDVR
                     FFELYDENNELRGSFYLDLYARENKRGGAWMDDCVGQMRKADGSLQKPVAYLTCNFNR
                     PVNGKPALFTHDEVITLFHEFGHGLHHMLTRIETAGVSGISGVPWDAVELPSQFMENW
                     CWEPEALAFISGHYETGEPLPKELLDKMLAAKNYQAALFILRQLEFGLFDFRLHAEFR
                     PDQGAKILETLAEIKKLVAVVPSPSWGRFPHAFSHIFAGGYAAGYYSYLWADVLAADA
                     FSRFEEEGIFNRETGQSFLDNILIRGGSEEPMELFKRFRGREPQLDAMLEHYGIKG"
     gene            10205..10957
                     /gene="rsmJ"
                     /locus_tag="ACHKBL_002663"
     CDS             10205..10957
                     /gene="rsmJ"
                     /locus_tag="ACHKBL_002663"
                     /EC_number="2.1.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709277.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1516)-N(2))-methyltransferase
                     RsmJ"
                     /protein_id="HFO5819516.1"
                     /translation="MKICLIDETGAGDGALSVLAARWGLEHDEDNLMALVLTPEHLEL
                     RKRDEPKLGGIFVDFVGGAMAHRRKFGGGRGEAVAKAVGIKGDYLPDVVDATAGLGRD
                     AFVLASVGCRVRMLERNPVVAALLDDGLARGYADAEIGGWLQERLQLIHASSLTALTD
                     ITPRPQVVYLDPMFPHKQKSALVKKEMRVFQSLVGPDLDADGLLEPARLLATKRVVVK
                     RPDYAPPLANVATPNAVVTKGHRFDIYAGTPV"
     gene            complement(11006..12475)
                     /gene="dtpB"
                     /locus_tag="ACHKBL_002664"
     CDS             complement(11006..12475)
                     /gene="dtpB"
                     /locus_tag="ACHKBL_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpB"
                     /protein_id="HFO5819517.1"
                     /translation="MNTTTPMGMLQQPRPFFMIFFVELWERFGYYGVQGVLAVFFVKQ
                     LGFSQEQAFVTFGAFAALVYGLISIGGYVGDHLLGTKRTIVLGALVLAIGYFMTGLSL
                     LKPDLIFIALGTIAVGNGLFKANPASLLSKCYPPKDPRLDGAFTLFYMSINIGSLIAL
                     SLAPVIADRFGYSVTYNLCGAGLIIALLVYIACRGMVKDIGSEPDFRPMSFSKLLYVL
                     LGSVVMIFVCAWLMHNVEVANLVLIVLSIVVTIIFFRQAFKLDKTGRNKMFVAFVLML
                     EAVVFYILYAQMPTSLNFFAINNVHHEILGFSINPVSFQALNPFWVVLASPILAGIYT
                     HLGNKGKDLSMPMKFTLGMFMCSLGFLTAAAAGMWFADAQGLTSPWFIVLVYLFQSLG
                     ELFISALGLAMIAALVPQHLMGFILGMWFLTQAAAFLLGGYVATFTAVPDNITDPLET
                     LPVYTNVFGKIGLVTLGVAVVMLLMVPWLKRMIATPESH"
     gene            complement(12790..13224)
                     /gene="uspA"
                     /locus_tag="ACHKBL_002665"
     CDS             complement(12790..13224)
                     /gene="uspA"
                     /locus_tag="ACHKBL_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HFO5819518.1"
                     /translation="MAYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYS
                     DLYTGLIDVNLGDMQKRISEETHHALTELSTNAGYPITETLSGSGDLGQVLVDAIKKY
                     DMDLVVCGHHQDFWSKLMSSARQLINTVHVDMLIVPLRDEEE"
     gene            13615..13950
                     /gene="uspB"
                     /locus_tag="ACHKBL_002666"
     CDS             13615..13950
                     /gene="uspB"
                     /locus_tag="ACHKBL_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspB"
                     /protein_id="HFO5819519.1"
                     /translation="MISTVALFWALCVVCIVNMARYFSSLRALLVVLRNCDPLLYQYV
                     DGGGFFTSHGQPNKQVRLVWYIYAQRYRDHHDDEFIRRCERVRRQFILTSALCGLVVV
                     SLIALMIWH"
     gene            complement(14021..15520)
                     /gene="pitA"
                     /locus_tag="ACHKBL_002667"
     CDS             complement(14021..15520)
                     /gene="pitA"
                     /locus_tag="ACHKBL_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitA"
                     /protein_id="HFO5819520.1"
                     /translation="MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMRSQLAVVMAAVFNFLGVLLGGLSVAYAIVHMLPTDLLLNMGSSHGLAMVFSMLL
                     AAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVLSIFGSLI
                     VSPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSA
                     IGVAFSHGANDGQKGIGLVMLVLIGVAPAGFVVNMNATGYEITRTRDAINNVEAYFEQ
                     HPALLKQATGADQLVPAPEAGATQPAEFHCHPSNTINALNRLKGMLTTDVESYDKLSL
                     DQRSQMRRIMLCVSDTIDKVVKMPGVSADDQRLLKKLKSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVATTIGEKIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVL
                     SSSVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAVLLSGGLYWLSLQFL"
     gene            15752..16945
                     /gene="yhiN"
                     /locus_tag="ACHKBL_002668"
     CDS             15752..16945
                     /gene="yhiN"
                     /locus_tag="ACHKBL_002668"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFO5819521.1"
                     /translation="MERFDAIIIGAGAAGMFCSALAGQAGRRVLLIDNGKKPGRKILM
                     SGGGRCNFTNLYVEPGAYLSQNPHFCKSALARFTQWDFIDLVNKHGITWHEKTLGQLF
                     CDDSAQQIVDMLVDECEKGNVTFRLRSEVLSVAKDETGFTLELNGMTVGCEKLVIATG
                     GLSMPGLGASPFGYKIAEQFGLNVLPTRAGLVPFTLHKPLLEELQVLAGVAVPSVITA
                     ENGIVFRENLLFTHRGLSGPAVLQISSYWQPGEFVSINLLPDVDLETFLNEQRNAHPN
                     QSLKNTLAVHLPKRLVERLQQLGQIPDVSLKQLNVRDQQALISTLTDWRVQPNGTEGY
                     RTAEVTLGGVDTNELSSRTMEARKVPGLYFIGEVMDVTGWLGGYNFQWAWSSAWACAQ
                     DLVAE"
     gene            complement(17032..18084)
                     /gene="yhiM"
                     /locus_tag="ACHKBL_002669"
     CDS             complement(17032..18084)
                     /gene="yhiM"
                     /locus_tag="ACHKBL_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2776 family protein"
                     /protein_id="HFO5819522.1"
                     /translation="MNIYIGWLFKLIPLLMGIICIALGEFVLTGSGQSEYFVAGHVLI
                     SLSAICLALFTTAFIIISQLTHGMNKFYNRLFPVIGYAGSATTMIWGWSLLASNNVMA
                     DEFVAGHVIFGVGMIAACVSTVAASSGHFLLIPKNASGSKSDGTPLQAYSSTIGNCLI
                     AVPVLLTLFGFIWSVILLRSADITPHYVAGHVLMGLTAICACLIGLVATIVHQTRNTF
                     SVKEHWLWCYWVILLGSLTIIFGIYVLISSDASARLAPGIILICLGMICYSIFSKVWL
                     LALVWRRTCSLANRIPMIPVFTCLFCLFLAAFLAEIAQTDMAYFIPSRVLVGLGAVCF
                     TLFSIVSILEAGSAKK"
BASE COUNT         4792 a         4507 c         4626 g         4325 t
ORIGIN      
        1 atacaggctt tgaatatctt ttatattagt cggtaagtca gctcgttgtt cagtaataaa
       61 attagtatct gaaaacatgg tttggacact gaatcttact ggtattccct cttctacgct
      121 aattttagtt atgattgcct tgtcgggttt aggattagcg acaacaatga ttcgatattc
      181 agcacttttg tttttttcgt atctaatgtt attaatctga ttaagtattg attgtgtctg
      241 ttgcgtaagt tgtgtcgaac ttatcgtttc aatgttagta ataagactta caagttgagc
      301 cacaactttt gagtagtctg catttgtata tgtagtgttc gtgtggcacg cattaatttg
      361 ttgaagttgg tattgcgcta aatcatattg atcagaggta tatccttctc cagagaatgt
      421 ggaattaatg ggttgttgtt cttcccaatt atcgcaaggc cacttcggtg tgtctggtgt
      481 tttaacattg tcggtatctg ttgtgcggtt ataaatatcg ccgtgatatg tatttatcat
      541 acatggggta aagtcaattg acataacatt ccttattgtt catctatttc gctaaatttt
      601 tgtaaatcgg agaattagag caggaatgga ttttcttcta cgcggaaata aacacaatgc
      661 gtcatatctt cctgatataa ttacctttaa cttccctttc atttccaccc ggtaatattt
      721 gtgtataaaa aaagggcagt gatagtatcg taaaggaaaa cgttaacaac tatcaaaaag
      781 cgctcatttt aaaaagttat gatgttcacc gatagtgtgt gaagtgatat ttttgtgatg
      841 ttttagatgt gtaataatga accaggttaa agtgtattta aaaatgtgtt gtattggcga
      901 ctgaaatctg ccctttaaaa ggggggatag cactgaaaca aagagacatt gcttctccga
      961 taaaatgtca gagaagcatg attgcgtggt gattgtcgat tgataactta ctgacggatc
     1021 cgtcatcatt ctcaaagcat tagttaagtt atttgtgatt tttaaggatt atcctatcta
     1081 tgattttcta ctcagaagga taaggcactc acaatgctcc ttatagttta ctgctggcat
     1141 ctatttaaaa tagcatttga gtcaaatagc ccatcaagat agccatacct aatataaccg
     1201 tcaggaatgc aactatcatc ggtattctga acattgattt aagtaatatc acttccgtca
     1261 ggcttgcgcc ggcactgccg atgattaatg ccattaatgc tcccagaccc attccttttg
     1321 tcatcagaac agatgccaga gggataaccg cctctgcccg gatatatagc ggaataccga
     1381 caacggcgct caatgggatg gcgaagggat tatctgcccc tgcatgagcg gcaatccact
     1441 ctgagggaat aaagccataa ataaaagagc ctattaatac ccctaacaaa agatatggca
     1501 gtacatcttt aaactgttgc aacgcatctt tggtggctag ttttatggga ttaatactta
     1561 ccacagcttt ggtactgcaa cagtttacta caggtttctc aatggcttta gccgctggac
     1621 tacagcaact cacttttatt ggcgtatatg tcgtccccgg cgaagttttg gctggagcac
     1681 aacaatttgc tgatgagctt ttactggcaa tgatatgacg ttcaaatccc agagaatcga
     1741 ggataatact ggcaagtacg gagacgccgg cggcgataat cgcatacaac aaggtaactt
     1801 tccagccaaa ggtcacccac attaacccga cgataatggg attaagtaat ggggaaacaa
     1861 ataaaaaagt gagggtcgga ccaaacccgg ctttcgctga taacaatcca cgtaacatag
     1921 ggattgtcga gcaactacag aacggggtga cggctcccaa cagagcagcc aggagatagc
     1981 ctttcccttt tcttgccccc atcatctgct ggattttatg gtctggcacc ttttgtctta
     2041 tcaggctcac accggcacta atcactataa acagcaaaga aagctcgacg gcgagaaaca
     2101 caaacatctc tgtggcatct tgcagcatcg caagccaact actcatacat aacctcattg
     2161 aataaatcta tatttccaga ataatcgaaa tgaaattcag atcaagatat ttctggtatt
     2221 atcgaaatat caaccagaca gagggggatc atatgcagtt ggaagaagtc gctaaagcgc
     2281 tgaaagagct aggtcacccg acccgtttgt tcatatttaa acatcttgtc aaagccgggg
     2341 aacaagggtt gccggtcggt gagttgcaaa agcagcttgg gatacccggc tctacactca
     2401 gtcaccatat ttcagcactg gtatcggtag ggctggtcaa acaaaaccgt gagagtcgaa
     2461 cgctgatgtg tgtttcgcaa tacgcaatgc tggaagcgat aattgaattc ttacgcgagg
     2521 agtgctgcgt aaatagcaaa attgcctgat gcgctacgcg ctacagattt catgccatgc
     2581 acggttttgt aggtcggata agacgcgtta gcgtcgcatc cgacataaac aaagcgcact
     2641 ttaccaacta tgggcagtgg atatcgccgc tttatttcaa gcgtttaccc gcttcatcaa
     2701 ccactttctc gccatcttcc ttggtgaacg cgccttgttg cgcatctggc agaatttcca
     2761 gcacgacttc tgaagggcgg cacaggcgag ttcccaacgg cgtcaccaca atcgggcgat
     2821 taatcagaat cgggtgctga agcataaagt cgattaaccg atcgtcagta aatttatctt
     2881 ctgcaaggcc cagttcctca tacggctcga cgtttttacg cagcagcgcg cgtacggaaa
     2941 tccccatatc ggcaatgagt ttgaccagtt catcacgcgt cggtggggtt tccagataat
     3001 agacaatggt cggttctgtg ccgctgttgc ggatcatctc cagcgtgtta cgcgacgtgc
     3061 cgcaggccgg gttgtgataa atggtaatgt tgctcatatc aatatctcat tacaaagtga
     3121 aagagagacg tagcgccagc gcagtcagcg tcacaaacag cacaggcagg gtcatgataa
     3181 tccctgtacg gaagtaatat ccccagctga tagtcatatt cttctgcgaa agtacgtgca
     3241 gccagagcag cgtcgccagg ctaccaatcg gggtaatttt cggccccaaa tcgcagccaa
     3301 ttacgttggc ataaaccatc gcttctttga taacgccaga tgccgtgctg ccatcaatgg
     3361 acaacgcgcc aaccagtacc gtcggcatat tgttcataat agaagagagg aaggcggtga
     3421 ggaatccggt gccgagcgtc gcggcccaca ggccattatc cgccagcacg ttgagtacgc
     3481 cagaaagata ttccgttagt ccggcattgc gcaggccata aaccaccaga tacatgccga
     3541 gcgagaagat gacaatctgc cagggcgcac cgcgtagcac cttactcgta ttaatcgcat
     3601 gaccgcgttt agcgacgaca aataatatca acgcgcccac tgccgcaatg gcgctgaccg
     3661 gaatgccgag cggttccagg acgaaaaatc ccaccagcag aaacagtaaa acaacccagc
     3721 cagttttgaa ggtggcagga tctttaatcg cttctgcggg agatttcagc agcgccatat
     3781 cgtagttctg cggaatatct ttgcggaaat agagatgcaa aatcaccagc gtggcaacaa
     3841 ttgcggcgat atccaccggc accatcaccg atgcgtattc gcgaaagccg aggccaaaga
     3901 aatctgcgga aacgatattc accaggttgg agacaataag cggcaggctg gcggtatcgg
     3961 caatgaatcc ggccgccatc acgaacgcca gcgtagtgcc tttactgaac cctaaagcca
     4021 gcaacatggc gatgacaatc ggtgtcaaaa taagcgccgc gccatcattg gcaaacagag
     4081 ctgcaacggc agcaccgagc aggacaatcc aggtgaacag caagcgaccg cgtccattgc
     4141 cccagcgtga gacgtgcagc gccgcccatt caaaaaagcc ggactcatcc agcagcaggc
     4201 tgatgataat gacggcaata aacgccgccg tcgcgttcca gacgatattc cacaccaccg
     4261 gaatatcacc cggatggacc acgcccgtaa ctaacgccag tactgcgccg agcgttgcac
     4321 tccagccgat gcctaaacct ttcggctgcc agataaccaa tacaatggtc aggacaaaga
     4381 tagcgcctgc cagtaacata atgcctcccg gataaaacac atgcgaaaat acatatgtgt
     4441 ttagctaaat tttttaactg caaatgttct tactgtctgc ggaacaattt tgtcgagcca
     4501 ggttgcggac aatcacctga accttttcct gttcacatcg ccaggcctca tcaataattt
     4561 tcgccgccca tgctggaata tgcggtgata agcggtaatg aacccactta ccttgcttgc
     4621 gatccagcaa tagcccactt tcacgcagca atgacagatg gcgggatatc ttgggctgcg
     4681 actgatcgag ggcagtgcag agatcgcaga cgcataactc tcccagttcg ctaagcagta
     4741 aaacgatgcc cagacgggtc tcatcagcaa gaattttgaa cagttgaatg ggtaacagaa
     4801 atgacatatt gcgctcctga ttgttgcagg tagtgtctct cttcgaagcg gataagtcaa
     4861 aaacatatat ggtttaacga atgtgtaagt gcagaggaag gtaataggtg tgaattttga
     4921 gttgggtatt catttgaaag gaggtctgaa tcaggacgta aaaaaaccga ctttgcgtcg
     4981 gttttttact ttccagccct gagttggtgg ctctggctgg agtgagaatg cccacatcta
     5041 acggttcaat actattagcc agtggctaac attgcaagcg ttagcgattt cttcagctgg
     5101 cggagggaca gtttgctata catgttggtg aaagaggatt tatatacttt catcgggtcg
     5161 ctgccgtacc aggccttgtc cgaatccgga tgtaaaaaac cgactttgcg tcggtttttt
     5221 actttccagc cctgagttgg tggctctgac tggagtgaga tagccatcat ctaacgcatc
     5281 aatgctaata ccatactgaa actattgcaa ggacatgctg gttttataac ccgcaagtac
     5341 tgtatgatta tccagttagc tctgagacat tttcactctg gcaatgcgca taaacgcttt
     5401 caaagtcctg gtcagaagta cgggtggtgc cgttaactga tgctctggcc ggagtgagaa
     5461 tgcccacatc taacaatggg gcatgcgccg tgacaggcag tggatgagta agcggatgca
     5521 ttctcactcc atcgcatgga gaaaacgggt gattgataaa gcaatcatcg ttcttggggc
     5581 gttaattgcg ctgctggaac tgatccgctt tctgcttcag cttctgaact gatagcggaa
     5641 acgtagctaa gggctaagag cacactactc ttagcccttt aacatttaac gcattgtcac
     5701 gaactcttct gccgctgtcg ggtgaatggc aaccgtgttg tcgaagtctt ttttggttgc
     5761 gcccatcttc agcgccaccg cgaagccctg caacatttca tccataccaa aaccaatgcc
     5821 gtgaattccg acaatcttct cttccggtcc aacgcatacc agtttcatgc ggcacggctg
     5881 gcggtgagtg gtgacggcgg tatacatcgc ggtgaaagag gatttataca ctttcacctg
     5941 atcgtcgccg tactgctcgc gcgcctgcgg ttccgttaaa ccaacagtcc caatcggcgg
     6001 atggctgaag accacggtcg gaatgttgct gtaatccaga tgctcatccg gcttgttatt
     6061 aaacaggcgt tcagagagac ggcgacccgc tgcaactgcc accggtgtca gttccactgc
     6121 acccgtgtta tcgcccaccg cgtaaatacc ttcaacgttg gtattttgat atttatcgac
     6181 gacgatatag cctttttcgt tagttttaac gccagcggct tccaggttga tgttgtcatt
     6241 ggcaggctcg cgaccaatcg cccaaatcag gcaatccacc gtttcactgc gcccatcttc
     6301 cagctccagc gtcaggctac catcagcatt tttcactact gctttcggga tggcgttggt
     6361 atgcaactgc gggccttcgg cgttcatcac ttcgaccagc gtttcagaga tcatcgggtc
     6421 gaagctgcgc agcggcgcat gtttacgcac aaacagatgc gttttcgcgc cgaggccgtt
     6481 aatcacgccc gccagttcca cggcgatgta acctgcgcca acaaccgcca cgcgctctgg
     6541 caaagcagga agggcgaaga agccatcaga atcaataccg tattccacgc ctggaatatc
     6601 cgggtggctc ggacgaccgc cggtggcgat cagaatatga tcggccgtga tggtttcgcc
     6661 gtttacctcc agcgttttgg catcaacgaa gcgggcaaag cctttgatta catcaacgtt
     6721 atttttaccg agcacgtttt cataggaagt atgaatacgg tcgatatagg cggtacggct
     6781 ggcgatcaac gtttcccagt tgaatttatt gatagtggta tcgaaaccat aatccggacc
     6841 gtacatgtgg atcgcttcac ggatttgcgc cgcgtgccac atcacttttt tcggcacgca
     6901 gccgacgttc acgcaggtgc cgcccagttc tttggcttca atcagcgcgc atttctgacc
     6961 gtacatagcc gcgcggttga tggaggcgat accgccgctg ccgccgccga tggcgatgta
     7021 atcatagtgt ttagtcatga catattgtcc ttatcgttga ttaccgcgat tgtagcgcga
     7081 gacgtaatag gtgccagcga tggctgcaat tactccggca cgatccagct tgcggtggcg
     7141 tgcccagtgc cagctggaac cagtttactg tgcagccacg gcagcacgtt attcatctgc
     7201 tgttccagct tccacggcgg attaatcaca atcatgccgg aagcggtcat gccacggcga
     7261 tcgctgtctg gcagcaccgc cagttcaatt tgcagaattt tgcgaatgcc ggtcgcttcc
     7321 aggtcgtgga tcatgcgctt aatttgctgg cgcagcacca ctggatacca caatgcgtaa
     7381 gtgccagtgg cgaaacgttt gtaaccttct gctatcccgc tgaccaccgc ctgatagtcg
     7441 gttttcattt catacggcgg gtcgataagg attaaaccac ggcgggaaac cggcggcagc
     7501 ttggccttaa gctgctggaa accgtcggct ttttcgacgc gcgcacggct gtctttctga
     7561 aattcagaac gcagcaacgg gtaatcgctc gggtgcagct cggtcagttg caggctgtcc
     7621 tgttcacgca gtagctggcg agcaatcaac ggcgaaccgg ggtaataacg caactgcccg
     7681 ctacggttga agtgttttac cacattgatg tacgcctcca gctccgcagg caaatcgtcc
     7741 tgctgccaga tacgggcgat accttcgaga tattcgccgg tacgctcggc atgttcgctg
     7801 cttaactgat aacgcccggc ccctgcgtgg gtgtcgagat agagaaacgg tttatctttc
     7861 tctttcagcg actcgatgat caggctctga acggtatgtt taaggacgtc ggcgtggttg
     7921 ccagcgtgaa agctgtggcg ataactgagc atgggtaaag gtgttccggt aagtaaaaat
     7981 cggcccgtat tcggggcgca caaaagccta tcaggacagt ataccgaaaa gaggccgccg
     8041 ccgcgaaagc gtaacgtttc tcattgaaat tcactacact taaccccatg ctacacacat
     8101 tatgtaaagc gcctgttgag cgcttcctta acctctttaa ccaggactgc gctaatgacg
     8161 aatccgttac tgactccctt tgaattgcct ccgttttcta aaattctccc ggaacatgtc
     8221 gttccagccg tgactaaggc gctgaatgac tgccgcgaaa acgtggagcg cgtagtagcg
     8281 caaggggcac cgtacacctg ggaaaatctc tgccagccgt tggcagaagt agacgatgtg
     8341 ctggggcgta tcttctcccc ggtcagccac ctgaactcgg tgaaaaatag cccggaactg
     8401 cgtgaagcgt acgaacaaac cctgccgctg ctgtcagaat acagcacctg ggtagggcaa
     8461 catgaagggc tgtataaagc gtatcgcgac ctgcgcgatg gcgatcatta cgccacgctg
     8521 aacacggcgc agaaaaaagc ggttgataac gcactgcgcg acttcgaact ctctggcatc
     8581 ggtctgccaa aagagaaaca acaacgctac ggtgagattg ccacccgtct ttccgagctg
     8641 ggcaaccagt acagcaacaa cgtcctcgat gcgacaatgg gctggaccaa actcgttacc
     8701 gacgaagcgg aactggcagg aatgccagaa agcgcactgg ctgcggcaaa agcccaggct
     8761 gaagcgaaag agctggaagg ttatttgctg acgctggata tcccaagcta cttgccggta
     8821 atgacttact gcgacaacca ggccttgcgt gaagagatgt atcgtgctta cagcacccgc
     8881 gcttccgatc aaggcccgaa cgccggtaag tgggacaaca gcaaggtgat ggaagagatc
     8941 ctcgcgctgc gtcacgaact ggcgcaactg ctgggttttg aaaactacgc ctttaaatcc
     9001 cttgccacta aaatggcaga aaatccgcag caggtgcttg atttcttaac cgatctggca
     9061 aaacgcgcgc gtccgcaagg tgaaaaagag ctggcgcaac tgcgtgcctt cgctaaagcc
     9121 gaatttggcg tcgatgagtt gcagccgtgg gatatcgctt actacagcga aaaacaaaaa
     9181 cagcatctct acagcatcag tgacgaacag ctgcgtccgt acttcccgga aaacaaagcg
     9241 gttaacggct tgtttgaagt ggtgaaacgt atttacggca tcaccgctaa agagcgtaaa
     9301 gatgttgatg tctggcatcc ggatgtacgt ttcttcgaac tgtatgacga gaacaacgaa
     9361 ctgcgcggca gcttctacct cgacctgtat gcccgtgaaa acaagcgcgg cggggcgtgg
     9421 atggatgact gcgtaggcca gatgcgtaaa gccgacggtt cgctgcaaaa accggtcgcg
     9481 tatctgacct gtaacttcaa ccgcccggta aatggtaaac cggcactgtt tacccatgac
     9541 gaagtgatca ccctgttcca tgaattcggt cacggcctgc accatatgct gacccgcatt
     9601 gaaactgctg gtgtatctgg tatcagcggg gtgccgtggg atgcggtcga actgccgagt
     9661 cagtttatgg aaaactggtg ctgggagccg gaggcgctgg cgtttatctc cggtcactat
     9721 gaaaccggcg aaccgctgcc gaaggagctg ctggataaaa tgctggcggc taagaactac
     9781 caggcggcgc tgtttattct gcgccaactg gagttcggcc tgtttgattt ccgcctccat
     9841 gccgagttcc gcccggatca gggagcgaaa atcctcgaaa ctctggcaga aatcaagaaa
     9901 ctggttgccg tagtaccgtc tccatcctgg ggccgtttcc cgcacgcttt cagccatatt
     9961 ttcgccggtg gttatgccgc aggttactac agctacctgt gggccgacgt gctggcggca
    10021 gatgccttct cgcgttttga ggaagagggc attttcaacc gtgaaactgg gcagtcgttc
    10081 ctcgacaaca ttctgatccg tggcggttca gaagagccga tggagctgtt caaacgcttc
    10141 cgtggtcgtg aaccacagct ggatgcgatg ctggagcatt acggcattaa gggctgaaca
    10201 ttcagtgaaa atctgcttaa ttgatgaaac aggcgccgga gacggcgcct tatctgttct
    10261 ggcggcccgc tgggggctgg agcacgatga agacaacctg atggcgctgg tgttaacacc
    10321 ggagcacctg gaactgcgca agcgtgatga gccgaagctg ggcggcatct ttgttgattt
    10381 tgttggcggg gcgatggcgc accgacgcaa attcggcggt ggtcgcggag aggcggtggc
    10441 gaaagcggtg ggcattaaag gcgattattt gccggatgtg gtggacgcca ccgcagggct
    10501 ggggcgcgat gcctttgtgc tggcttcggt cggctgccgc gtgcggatgc tggagcgtaa
    10561 tccggtggtt gctgcattgc tcgacgacgg cctggcgcgt ggttatgcgg atgcggaaat
    10621 cggcggctgg ttgcaggagc ggttgcagtt aattcacgct tccagcctga cggcgctgac
    10681 cgatattact ccgcgcccgc aggtggtcta tctcgatccg atgttcccgc ataagcagaa
    10741 aagcgcgttg gtgaaaaaag agatgcgtgt ctttcagtcg ctggtggggc cggatcttga
    10801 tgccgatgga ttgctggagc ctgcacgctt gctggcgacc aaacgcgtgg tggtaaagcg
    10861 cccggactac gcgccgccgc tggcgaatgt cgccacgcca aacgcggtag tgaccaaagg
    10921 gcatcgcttt gatatttatg ccggaacgcc ggtgtaaaag taaagcaggg tagcgttatc
    10981 gctaccctgc tttctgcaag aataattaat ggctttcggg cgtcgcaatc atgcgtttca
    11041 gccacggcac catcagcagc atcaccaccg caacgcccag cgtaaccagg ccaatcttac
    11101 caaacacgtt ggtatagacg ggcaacgtct caagcggatc ggtaatgttg tctggtactg
    11161 cggtaaatgt tgccacatag ccgcccagca agaacgcggc agcctgcgtc aggaaccaca
    11221 tcccgagaat aaagcccatc aaatgctgcg gcaccagggc agcaatcatc gccagaccca
    11281 gggcgctaat aaacaattca cccaggctct ggaacaagta aaccagcacg ataaaccacg
    11341 gcgatgtcag tccttgtgca tcagcaaacc acattcccgc agcggcggct gtaagaaagc
    11401 ccagcgagca cataaacatg ccgagagtaa atttcatcgg catcgagagg tctttgcctt
    11461 tgttacccag atgcgtgtaa atgcctgcca gtattgggct ggcgagtacc acccagaacg
    11521 ggttaagcgc ctggaagctg actgggttga tggaaaagcc gagaatttca tgatgcacgt
    11581 tgttgatagc aaagaagttc agcgatgttg gcatctgggc gtagagaatg taaaacacca
    11641 ccgcttcgag catcaggaca aaggcgacaa acattttatt gcgcccggtt ttatccagct
    11701 tgaatgcctg acgaaagaaa atgatggtga cgacgatgga gagaacaatc agcaccagat
    11761 tggcgacttc tacgttgtgc atcagccatg cacagacgaa gatcatcacc acgctgccaa
    11821 gtaacacgta caacagtttg ctgaagctca tcggccggaa gtcgggttca gaaccaatgt
    11881 ctttcaccat tccgcgacag gcgatgtaaa ccaatagcgc gataattaat cccgcaccgc
    11941 acaggttgta ggtgactgaa tagccgaatc tatcagcgat cacaggggcc agcgataacg
    12001 ctatcaacga gccgatgttg atcgacatat agaacagggt aaatgcgcca tcaagccgcg
    12061 gatctttcgg cggatagcac ttcgaaagca agctggctgg gttagcttta aacaggccgt
    12121 taccgacagc gatagtcccc agggcgatga aaatcaggtc aggcttaagt agcgacaggc
    12181 cggtcatgaa gtagccaatc gccagcacaa gtgctccgag gacaatggtg cgtttggtcc
    12241 ccagcaggtg gtcgccgaca tagccgccaa tggaaatgag gccatagacc agcgcagcaa
    12301 aagcaccaaa agtgacaaaa gcctgttctt gcgagaatcc aagctgctta acgaagaaaa
    12361 ccgccagtac gccctgcacg ccgtagtagc cgaatcgctc ccataactcg acaaaaaaga
    12421 tcatgaaaaa tgggcgaggt tgctgcagca tccccatggg tgttgttgta ttcatattta
    12481 ttaaccttcc aataccatcc tgaaaagtat gaacgcgtaa gggataacgc gcgaaaaagt
    12541 ttacatctgt cagagattct taatattgat gcggtgaata gattaataca ctattacaac
    12601 agaaaataac cagctgatca cactaatgat ggaaaatatg atggtgttaa atgttgtatt
    12661 ggcaggggat tttgattgcg tacgcagacc gtggccagat aaggtgttta cgctgcaagg
    12721 taacaaggcg cgccaaaaag caaaacgccc gcatatgcgg gcgttcagga acacgtcgta
    12781 gagggaagat tattcttctt cgtcgcgcag cggaacaatc agcatatcaa cgtgaacggt
    12841 gttgatcagc tgacgtgcgg aagacatcag tttgctccag aagtcctggt ggtgaccaca
    12901 aaccaccaaa tccatatcgt atttcttgat tgcatcgacc agaacctggc ccaggtcgcc
    12961 gctgccgctc agggtttcag tgattgggta gcctgcatta gtggaaagct cggtcagcgc
    13021 atgatgtgtc tcttcagaga tgcgtttctg catatcaccc agattcacat caataagccc
    13081 ggtgtatagg tcagagtagt ttacatctac gtggatcaga gaaactttcg cattgtaggg
    13141 gcgagccata gagactgctt tctctaccag aactttgctt tccggggaga gatcgaccgc
    13201 gatgagaatg tgtttataag ccatagtgtt actccttcca taaagttgtc gatgactggc
    13261 cagctagcgt ttcttgtgcc gttacgtcac ccgcgtcctg cgaactacgt gcgcggggct
    13321 aacccagccg taaagattca gtgttaagac catccttacc ttatagcgac ccggatgatc
    13381 cgtcaatccg ccttgcttac ccgttagata aacaaatttt cgacatgaat atattgatag
    13441 tggttaacct tctggaaaaa aaacaacctg atctcctaca ctatctatag agccgctcgg
    13501 atgttggtca ccacaaaagc ggtttctcca ggacctggtg ttaactgatt ggcagcgtat
    13561 cgggagcggt agccccgggg tggaactccg cgggcaggtc gccggggagg aggtatgata
    13621 agcaccgtcg cattattttg ggctttatgt gtcgtttgca ttgttaacat ggcgcgctat
    13681 ttctcatcac tacgcgcgtt gttagtggta ctgcgtaact gcgatccatt gctctatcaa
    13741 tatgttgatg gagggggctt ttttacctca catggccaac ccaacaaaca ggtgcgtctc
    13801 gtttggtata tctatgccca acgttatcgc gatcatcacg atgatgagtt tattcgccgc
    13861 tgtgagcggg tgcgtcggca gtttattctg accagcgcat tgtgtggtct ggtggtggtc
    13921 agtctgattg cattgatgat ttggcattaa gcacttcagc aaaaataacg cataaaaaaa
    13981 gcgggccagt aaactgaccc gctcgttttg gtgcgtgcga ttacaggaac tgcaaggaga
    14041 gccagtacag cccgccggaa agcagtaccg cagccggaag ggtaaacacc caggccatca
    14101 gaatactggt cacggtttta cgctgtaagc cgccgccatc taccaccatc gtccccgcga
    14161 cagaagagga gagtacgtgg gtagtggaaa ccggcatccc ggtataactc gccaggccga
    14221 tagacactgc cgcagtcatc tgggcagaca ttccctgagc gtaggtcatg cctttcttac
    14281 cgattttctc accgatagtc gttgccacac gacgccagcc aatcatcgta ccgataccta
    14341 acgccagcgc gaccgccatg atgatccaca ccggtgcata ctcgatggtg ctaagcatgt
    14401 cagacttcag tttcttcaac aggcgctgat cgtcagcact cacgccaggc atcttcacca
    14461 ctttgtcgat agtgtcagaa acgcacagca taatgcggcg catctggcta cgttgatcaa
    14521 gcgacagctt atcgtagctt tccacatcgg tagtcaacat gcctttcagg cggttgagcg
    14581 cgttaatggt attcgacgga tggcaatgga actccgcagg ttgcgttgcg ccagcttccg
    14641 gagccggtac taactgatca gcaccggtcg cttgtttcag cagcgcagga tgctgctcaa
    14701 agtaagcttc gacgttgttg atggcatcac gggtacgggt gatttcgtag ccagtggcat
    14761 tcatgttcac cacgaagcct gctggtgcga caccaatcaa taccaacata accagaccaa
    14821 tgcctttctg accatcgttc gcgccatgcg aaaacgccac gccgatagcg gaaaggatca
    14881 gcgcaatacg cgtccagaac ggcggctttt tcttgccgtc tttcttttca cgctccgctg
    14941 gggtcaggtg gatacgggcg cgtttcttgg tgccgctcca gtagcgacgc agcaagaaaa
    15001 tcagaccgcc agcaaacacc aggccgacaa taggggaaac gatcagagaa ccgaaaatac
    15061 ttaatacttt cgggatattg agtgcatcca ccactgacgt cccggtcatc aacgcattgg
    15121 ttaaaccaat cccgatgatc gcgccaatca gcgtatgaga gctggatgca ggtaaaccaa
    15181 agtaccaggt acccaggttc cagataatcg ccgccagcaa catagagaac accatggcaa
    15241 ggccatgaga cgatcccata ttaagcagca gatccgtcgg cagcatatgc acaatggcat
    15301 aggcaacact cagaccaccc agcaaaacac ccaaaaagtt gaacaccgcc gccataacca
    15361 cggcgagctg agaacgcatc gcgcgggtat agataacggt tgccacggcg ttggctgtgt
    15421 catggaaacc attgatggct tcgtagaaca gcacaaaagc cagtgcaagc aataataaca
    15481 gcccggtatg caaatccagg ccagcaaaca aatgtagcat aggacgttac gccattttga
    15541 ggacatgaac gcggcgcatt atcagtgact ttcgcggcgc gggcaaagtg aaatatagac
    15601 tttttttgat ttgcctcctg tatggatttc actcaaaaaa taattatctt atataattca
    15661 ggtaaatact tccttttagt aatattgatg ctggtgcgac cactgaggaa tctttacaat
    15721 tcacgcccgt tttttcttag aggagcgcaa agtggaaagg tttgatgcca ttattatagg
    15781 cgctggtgcg gcgggtatgt tctgttctgc gctggcaggt caggcaggac gccgggttct
    15841 gctgatcgat aatggtaaaa aaccagggcg caaaatcctc atgtctggcg gtgggcgctg
    15901 caactttacc aacctttatg tcgaaccggg cgcttatctg agccagaatc cgcatttttg
    15961 taagtctgca ctcgcgcgtt ttacccagtg ggatttcatt gatctggtca ataaacacgg
    16021 catcacctgg cacgagaaaa cgttagggca actcttctgc gatgactccg cgcagcagat
    16081 tgtcgacatg ctggtggatg agtgcgagaa gggcaatgtg accttcagat tgcgtagcga
    16141 agtgctgagt gtggcgaagg atgaaacagg cttcacgctt gaactgaacg gcatgactgt
    16201 cggttgcgaa aagctggtca tcgcgaccgg tggactgtca atgccagggc tgggcgcgtc
    16261 gccgtttggt tataagattg ccgaacaatt tggcctcaac gtgctgccga cccgcgcggg
    16321 cctggtgcca ttcactctgc ataaaccgtt gctcgaagag ttacaggtgc tggcgggcgt
    16381 ggcggtgcct tccgtgatta ccgctgaaaa cggcatcgtt ttccgtgaga acttactctt
    16441 cacccaccgc ggcttatctg gaccggcggt gttgcaaatt tccagctact ggcaaccggg
    16501 tgaatttgtc agtatcaatc tgctaccgga tgtggacctc gaaaccttcc tgaatgagca
    16561 gcgtaatgca catccgaatc agagcctgaa aaacacactg gcggttcatc taccgaagcg
    16621 gttggttgaa cgtttacaac aactcgggca aatcccggat gtttcgctaa aacagctcaa
    16681 cgtgcgtgac caacaggcac tgattagcac attgaccgac tggcgcgtac aacccaacgg
    16741 cactgaaggc tatcgcactg ccgaagtgac gctcggcggc gtggacacca acgaactctc
    16801 ttcacggacg atggaagcgc gcaaagtgcc tgggctgtac ttcatcggcg aagtgatgga
    16861 cgtcaccggc tggctggggg gctataactt ccagtgggcg tggtcgagtg catgggcttg
    16921 tgcgcaggat ttggtggcag aataagaagc aaaatgtgct ctcgatacca gagggattaa
    16981 cctgaataaa accagataag gcgtccaacg ccttatctga agcctttttt attatttttt
    17041 cgctgaaccc gcttctaata tagaaacgat ggaaaacagt gtaaaacaaa ctgcacctaa
    17101 gccaaccaga actcgcgaag gaataaagta agccatatcg gtttgcgcaa tttcagccag
    17161 gaacgcagca aggaacagac aaaacagaca ggtaaatacc ggaatcatgg gtattctgtt
    17221 ggctaacgaa caggtgcgtc gccataccag cgctaacaac cacactttgg agaaaatact
    17281 gtagcagatc atccccaggc aaatgagaat aatgccgggg gccaggcggg cgcttgcatc
    17341 tgaactgatt aatacgtaga taccaaaaat aatggtaagt gagccaagca aaataaccca
    17401 atagcaccac agccaatgtt ctttcactga gaacgtattc cgcgtttgat ggacaatagt
    17461 ggcgacaagg ccaataagac aagcgcaaat tgccgttaac cccattagta cgtgacctgc
    17521 aacataatgt ggtgtgatat ctgcactacg taacagtatg actgaccaaa tgaaaccaaa
    17581 gagagtaagc agaacgggaa cggcaattaa gcaattaccg attgttgaag aataggcctg
    17641 caatggcgta ccgtcactct tactgcccga ggcgtttttg gggatcaaca gaaaatgtcc
    17701 ggaagatgcc gctacggttg atacacaagc ggcaatcatg ccaacgccga aaataacatg
    17761 accggcgaca aattcatctg ccatcacatt attgcttgcc aacagtgacc atccccaaat
    17821 catagttgta gcagaaccgg cataaccaat aacgggaaat aacctgttgt agaatttatt
    17881 catgccgtga gttaactgag atataataat aaatgcggta gtgaacaatg ccagacatat
    17941 cgcagaaaga gaaatcagaa catgccctgc aacgaaatat tcactttgcc cagaaccggt
    18001 taatacaaac tcacctaatg cgatgcatat tatacccatc agtaaaggaa ttaatttaaa
    18061 caaccatccg atatatatat tcattttaaa ccccatatat attttctgct acacttcgaa
    18121 aaacatatga ttcgtctttt cagttagtta aatatgtcta taagatgcca tcaattaaaa
    18181 aaagtaactt cactataatc ggatttagcg ttatggggat caacttttag cggtgagtta
    18241 atgctattaa
//
LOCUS       DAZFLD010000093        18135 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.093, whole genome shotgun sequence.
ACCESSION   DAZFLD010000093 DAZFLD010000000
VERSION     DAZFLD010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18135)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18135)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18135
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.093"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(59..1150)
                     /gene="ychF"
                     /locus_tag="ACHKBL_002670"
     CDS             complement(59..1150)
                     /gene="ychF"
                     /locus_tag="ACHKBL_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HFO5819523.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVV
                     PMPDPRLDQLAEIVKPQRTLPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGH
                     VVRCFENDNIIHVSGKVNPADDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKA
                     ELAVLEKCLPQLENAGMLRALDLSAEEKAAIRYLSFLTLKPTMYIANVNEDGFENNPY
                     LDQVREIAAKEGSVVVPVCAAVEADIAELDDEEREEFMQELGLEEPGLNRVIRAGYKL
                     LNLQTYFTAGVKEVRAWTIPVGATAPQAAGKIHTDFEKGFIRAQTISFEDFITYKGEQ
                     GAKEAGKMRAEGKDYIVKDGDVMNFLFNV"
     gene            complement(1267..1851)
                     /gene="pth"
                     /locus_tag="ACHKBL_002671"
     CDS             complement(1267..1851)
                     /gene="pth"
                     /locus_tag="ACHKBL_002671"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HFO5819524.1"
                     /translation="MTIKLIVGLANPGAEYAATRHNAGAWFVDLLAERLRAPLREEAK
                     FFGYTSRVTLGGEDVRLLVPTTFMNLSGKAVAAMASFFRINPDEILVAHDELDLPPGV
                     AKFKLGGGHGGHNGLKDIISKLGNNPNFHRLRIGIGHPGDKNKVVGFVLGKPPVSEQK
                     LIDEAIDEAARCTEIWFTDGLTKATNRLHAFKAQ"
     gene            2129..2407
                     /gene="ychH"
                     /locus_tag="ACHKBL_002672"
     CDS             2129..2407
                     /gene="ychH"
                     /locus_tag="ACHKBL_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein YchH"
                     /protein_id="HFO5819525.1"
                     /translation="MKRKNASLLGNVLMGLGLVVMVVGVGYSILNQLPQFNMPQYFAH
                     GAVLSIFVGAILWLAGARVGGHEQVCDRYWWVRHYDKRCRRSDNRRHS"
     gene            complement(2462..4141)
                     /gene="dauA"
                     /locus_tag="ACHKBL_002673"
     CDS             complement(2462..4141)
                     /gene="dauA"
                     /locus_tag="ACHKBL_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HFO5819526.1"
                     /translation="MNKIFSSHVMPFRALIDACWKEKYTAARFTRDLIAGITVGIIAI
                     PLAMALAIGSGVAPQYGLYTAAVAGIVIALTGGSRFSVSGPTAAFVVILYPVSQQFGL
                     AGLLVATLMSGVFLILMGLARFGRLIEYIPVSVTLGFTSGIGITIGTMQIKDFLGLQM
                     AHVPEHYLQKVGALFMALPTINVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAGCA
                     VMGIVNLLGGHVATIGSQFHYVLADGSQGNGIPQLLPQLVLPWDLPNSEFTLTWDSIR
                     TLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKANSELVGQGLGNIIAPFFGGITATA
                     AIARSAANVRAGATSPISAVIHSILVILALLVLAPLLSWLPLSAMAALLLMVAWNMSE
                     AHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISVGIVLASLLFMRRIARMTRLAP
                     VVVDVPDDVLVLRVIGPLFFAAAEGLFTDLESRLEGKRIVILKWDAVPVLDAGGLDAF
                     QRFVKRLPEGCELRVCNVEFQPLRTMARAGIQPIPGRLAFFPNRRAAMADL"
     gene            complement(4266..5213)
                     /gene="prs"
                     /locus_tag="ACHKBL_002674"
     CDS             complement(4266..5213)
                     /gene="prs"
                     /locus_tag="ACHKBL_002674"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFO5819527.1"
                     /translation="MPDMKLFAGNATPELAQRIANRLYTSLGDAAVGRFSDGEVSVQI
                     NENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
                     LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVL
                     VDDMIDTGGTLCKAAEALKERGAKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIP
                     LSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH"
     gene            complement(5364..6215)
                     /gene="ispE"
                     /locus_tag="ACHKBL_002675"
     CDS             complement(5364..6215)
                     /gene="ispE"
                     /locus_tag="ACHKBL_002675"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HFO5819528.1"
                     /translation="MRTQWPSPAKLNLFLYITGQRADGYHTLQTLFQFLDYGDTISIE
                     LRDDGDIRLLTPVEGVEHEDNLIVRAARLLMKTAADSGRLPTGSGAEISIDKRLPMGG
                     GLGGGSSNAATVLVALNHLWQCGLSMDELAEMGLTLGADVPVFVRGHAAFAEGVGEIL
                     TPVDPPEKWYLVAHPGVSIPTPVIFKDPELPRNTPKRSIETLLKCEFSNDCEVIARKR
                     FREVDAVLSWLLEYAPSRLTGTGACVFAEFDTESEARQVLEQAPEWLNGFVAKGVNLS
                     PLHRAML"
     gene            complement(6215..6838)
                     /gene="lolB"
                     /locus_tag="ACHKBL_002676"
     CDS             complement(6215..6838)
                     /gene="lolB"
                     /locus_tag="ACHKBL_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein insertase outer membrane protein
                     LolB"
                     /protein_id="HFO5819529.1"
                     /translation="MPLPDFRLIRLLPLAALVLTACSVTTPKGPGKSPDSPQWRQHQQ
                     DVRNLNQYQTRGAFAYISDQQKVYARFFWQQTGQDRYRLLLTNPLGSTELELNAQPGN
                     VQLVDNKGQRYTADDAEEMIGKLTGMPIPLNSLRQWILGLPGDATDYKLDDQYRLSEI
                     TYSQNGKNWKVVYGGYDTKTQPAMPSNMELTDGGQRIKLKMDNWIVK"
     gene            7052..8308
                     /gene="hemA"
                     /locus_tag="ACHKBL_002677"
     CDS             7052..8308
                     /gene="hemA"
                     /locus_tag="ACHKBL_002677"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041689438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HFO5819530.1"
                     /translation="MTLLALGINHKTAPVSLRERVSFSPDKLDQALDSLLAQPMVQGG
                     VVLSTCNRTELYLSVEEQDNLQEALIRWLCDYHNLNEEDLRKSLYWHQDNDAVSHLMR
                     VASGLDSLVLGEPQILGQVKKAFADSQKGHMKASELERMFQKSFSVAKRVRTETDIGA
                     SAVSVAFAACTLARQIFESLSTVTVLLVGAGETIELVARHLREHKVQKMIIANRTRER
                     AQILADEVGAEVIALSDIDERLREADIIISSTASPLPIIGKGMVERALKSRRNQPMLL
                     VDIAVPRDVEPEVGKLANAYLYSVDDLQSIISHNLAQRKAAAVEAETIVAQETSEFMA
                     WLRAQSASETIREYRSQAEHVRDELTAKALAALEQGGDAQAIMQDLAWKLTNRLIHAP
                     TKSLQQAARDGDNERLNILRDGLGLE"
     gene            8350..9432
                     /gene="prfA"
                     /locus_tag="ACHKBL_002678"
     CDS             8350..9432
                     /gene="prfA"
                     /locus_tag="ACHKBL_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HFO5819531.1"
                     /translation="MKPSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQ
                     LSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQDELREAKEKSEQLEQQLQVLLLP
                     KDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYK
                     EIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDIN
                     PADLRIDTFRSSGAGGQHVNTTDSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGA
                     RIHAAEMAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVME
                     GKLDMLIEPIIQEHQADQLAALSEQE"
     gene            9432..10265
                     /gene="prmC"
                     /locus_tag="ACHKBL_002679"
     CDS             9432..10265
                     /gene="prmC"
                     /locus_tag="ACHKBL_002679"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFO5819532.1"
                     /translation="MEYQHWLREAISQLQASESPRRDAEILLEHVTGRGRTFILAFGE
                     TQLTDEQCQQLDALLTRRRDGEPIAHLTGVREFWSLPLFVSPATLIPRPDTECLVEQA
                     LARLPEQPCRILDLGTGTGAIALALASERPDCEITAVDRMPDAVALAQRNAQNLAIKN
                     IHILQSDWFSALAGQQFAMIVSNPPYIDEQDPHLQQGDVRFEPLTALVAADSGMADIV
                     HIIEQSRNALVSGGFLLLEHGWQQGEAVRQAFIHAGYHDVETCRDYGDNERVTLGRYY
                     Q"
     gene            10262..10654
                     /gene="sirB2"
                     /locus_tag="ACHKBL_002680"
     CDS             10262..10654
                     /gene="sirB2"
                     /locus_tag="ACHKBL_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion regulator SirB2"
                     /protein_id="HFO5819533.1"
                     /translation="MTSFSTLLSVHLISIALSVGLLTLRFWLRYQKHPQAFARWTRIV
                     PPVVDTLLLLSGIALMAKAHILPFSGQAQWLTEKLFGVIIYIVLGFIALDYRRMHSQQ
                     ARIIAFPLALVVLYIIIKLATTKVPLLG"
     gene            10658..11467
                     /gene="sirB1"
                     /locus_tag="ACHKBL_002681"
     CDS             10658..11467
                     /gene="sirB1"
                     /locus_tag="ACHKBL_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion regulator SirB1"
                     /protein_id="HFO5819534.1"
                     /translation="MRSLADFEFNKAPLCEGMILACEAIRRDFPSQDVYDELERLVSL
                     AKEEISQLLPLEEQLEKLIALFYGDWGFKASRGVYRLSDALWLDQVLKNRQGSAVSLG
                     AVLLWVANRLDLPLLPVIFPTQLILRIECPDGEIWLINPFNGESLSEHMLDVWLKGNI
                     SPSAELFYEDLDEADNIEVIRKLLDTLKASLMEENQMELALRTSEALLQFNPEDPYEI
                     RDRGLIYAQLDCEHVALNDLSYFVEQCPEDPISEMIRAQINNIAHKHIVLH"
     gene            11503..12357
                     /gene="kdsA"
                     /locus_tag="ACHKBL_002682"
     CDS             11503..12357
                     /gene="kdsA"
                     /locus_tag="ACHKBL_002682"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HFO5819535.1"
                     /translation="MKQKVVSIGDINVANDLPFVLFGGMNVLESRDLAMRICEHYVTV
                     TQKLGIPYVFKASFDKANRSSIHSYRGPGLEEGMKIFQELKQTFGVKIITDVHEPSQA
                     QPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFVSPGQMGNIVDKFKEGGN
                     EKVILCDRGANFGYDNLVVDMLGFSIMKKVSGNSPVIFDVTHALQCRDPFGAASGGRR
                     AQVAELARAGMAVGLAGLFIEAHPDPEHAKCDGPSALPLAKLEPFLKQMKAIDDLVKG
                     FEELDTSK"
     gene            complement(12506..12613)
                     /locus_tag="ACHKBL_002683"
     CDS             complement(12506..12613)
                     /locus_tag="ACHKBL_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFO5819536.1"
                     /translation="MTLAQFTVIFWHDLAAPILAGIITAAIVGWWRNRK"
     gene            complement(13018..14118)
                     /gene="chaA"
                     /locus_tag="ACHKBL_002684"
     CDS             complement(13018..14118)
                     /gene="chaA"
                     /locus_tag="ACHKBL_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-potassium/proton antiporter ChaA"
                     /protein_id="HFO5819537.1"
                     /translation="MSNAQEAVKTRHKETSLIFPVLALVVLFLWGSSQTLPVVIAINL
                     LALIGILSSAFSVVRHADVLAHRLGEPYGSLILSLSVVILEVSLISALMATGDAAPTL
                     MRDTLYSIIMIVTGGLVGFSLLLGGRKFATQYMNLFGIKQYLIALFPLAIIVLVFPMA
                     LPAANFSTGQALLVALISAAMYGVFLLIQTKTHQSLFVYEHEDDSDDDDPHHGKPSAH
                     SSLWHAIWLIVHLIAVIAVTKMNASPLETLLDSMNAPVAFTGFLVALLILSPEGLGAL
                     KAVLNNQVQRAMNLFFGSVLATISLTVPVVTLIAFMTGNELQFALGAPEMVVMVASLV
                     LCHISFSTGRTNVLNGAAHLALFAAYLMTIFA"
     gene            14388..14618
                     /gene="chaB"
                     /locus_tag="ACHKBL_002685"
     CDS             14388..14618
                     /gene="chaB"
                     /locus_tag="ACHKBL_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cation transport regulator ChaB"
                     /protein_id="HFO5819538.1"
                     /translation="MPYKTKSDLPESVKHVLPSHAQDIYKEAFNSAWDQYKDKEDRRD
                     DASREETAHKVAWAAVKHEYAKGDDDKWHKKS"
     gene            14776..15471
                     /gene="chaC"
                     /locus_tag="ACHKBL_002686"
     CDS             14776..15471
                     /gene="chaC"
                     /locus_tag="ACHKBL_002686"
                     /EC_number="4.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-specific
                     gamma-glutamylcyclotransferase"
                     /protein_id="HFO5819539.1"
                     /translation="MITRDFLMNADCKTAFGAIEESLLWSAEQRAASLAATLACRPDE
                     GPVWIFGYGSLMWNPALEFTESCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEGG
                     RTTGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGRTVNAIVFIMDPRHP
                     EYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNDLLVSVKKLLA
                     ENYPDGVLRPGLA"
     gene            complement(15515..15868)
                     /gene="ychN"
                     /locus_tag="ACHKBL_002687"
     CDS             complement(15515..15868)
                     /gene="ychN"
                     /locus_tag="ACHKBL_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/F sulfur relay family protein YchN"
                     /protein_id="HFO5819540.1"
                     /translation="MQKIVIVANGAPYGSESLFNSLRLAIALREQESNLDLRLFLMSD
                     AVTAGLRGQKPGEGYNIQQMLEILTAQNVPVKLCKTCTDGRGISTLPLIDGVEIGTLV
                     ELAQWTLSADKVLTF"
     gene            16054..17448
                     /gene="ychO"
                     /locus_tag="ACHKBL_002688"
                     /gene_synonym="iatA"
     CDS             16054..17448
                     /gene="ychO"
                     /locus_tag="ACHKBL_002688"
                     /gene_synonym="iatA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter invasin YchO"
                     /protein_id="HFO5819541.1"
                     /translation="MSRFVPRIIPFYLLLLAAGGTANAQSTFEQKAANPFDNNNDGLP
                     DLGMAPENHDGEKHFAEIVKDFGETSMNDNGLDTGEQAKAFALGKVRDAFSQQVNQHV
                     ESWLSAWGNASVDVKVDNEGHFTGSRGSWFVPLQDNDRYLTWSQLGLTQQDDGLVSNV
                     GVGQRWARGSWLVGYNTFYDNLLDENLQRAGFGAEAWGEYLRLSANFYQPFAAWHEQT
                     ATQEQRMARGYDLTARMRMPFYQHLNTSVSVEQYFGDRVDLFNSGTGYHNPVALSLGL
                     NYTPVPLVTVTAQHKQGESGENQNNLGLNLNYRFGVPLKKQLSAGEVAESQSLRGSRY
                     DNPQRNNLPTLEYRQRKTLTVFLATPPWDLKPGETVPLKLQIRSRYGIRQLIWQGDTQ
                     ILSLTPGAQANSEEGWTLIMPDWQNGEGASNHWRLSVVVEDNQGQRVSSNEITLTLVE
                     PFDALSNDELRWEP"
     gene            complement(17449..18099)
                     /gene="narL"
                     /locus_tag="ACHKBL_002689"
     CDS             complement(17449..18099)
                     /gene="narL"
                     /locus_tag="ACHKBL_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HFO5819542.1"
                     /translation="MSNQEPATILLIDDHPMLRTGVKQLISMAPDITVVGEASNGEQG
                     IELAESLDPDLILLDLNMPGMNGLETLDKLREKSLSGRIVVFSVSNHEEDVVTALKRG
                     ADGYLLKDMEPEDLLKALHQAAAGEMVLSEALTPVLAASLRANRATTERDVNQLTPRE
                     RDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVWVHQERIF"
BASE COUNT         4555 a         4513 c         4722 g         4345 t
ORIGIN      
        1 ccattatgtg cgactcacat aatggctcat tattaaccgt cagggtatta cgtggaattt
       61 agacgttgaa caggaagttc atcacatcgc catctttaac gatgtaatct ttaccttctg
      121 cacgcatttt gcctgcttct ttcgcgcctt gttcaccttt gtaagtgatg aaatcttcaa
      181 acgagatagt ctgtgcgcgg ataaagcctt tttcgaaatc ggtatggatt ttgccagccg
      241 cttgcggtgc agttgcgccg acggggatgg tccatgcgcg cacttctttc accccagcgg
      301 tgaagtaagt ttgcaggttc agcagtttat aaccggcacg gatcacacgg ttcaggcccg
      361 gctcttccag tcccagttcc tgcataaact cttcacgttc ttcatcgtcc agttcggcaa
      421 tgtctgcttc aacagctgca caaaccggaa ccacaacaga accttctttc gccgcgattt
      481 cacgcacctg gtcaagatac gggttgtttt caaaaccgtc ttcgttgacg ttggcgatgt
      541 acatcgtcgg tttcagcgtc aggaagctca ggtaacgaat agccgctttt tcttctgcgc
      601 ttaaatccag cgcgcgcagc atgcccgcgt tttccagctg tggaaggcat ttttccagta
      661 ctgccagttc ggctttcgcg tctttatcgc cgcctttggc tttcttctgt acgcggtgaa
      721 tcgcgcgctc gcaggtgtcg agatccgcca gtgccagttc ggtattgata acttcaatat
      781 cgtccgccgg gttaacttta ccggaaacgt gaatgatatt gtcattctca aagcagcgaa
      841 caacgtggcc gatcgcttcg gtttcacgaa tgttggtcag gaactggtta cccagacctt
      901 cacctttcga cgcgcccttt accagaccgg cgatatcgac aaattccatg gtagtgggaa
      961 gcgtacgctg cggttttacg atttcagcca gttgatccag gcgagggtca ggcattggta
     1021 cgacgcccgt gttcggctca atggtacaga atggaaagtt ggccgcttca ataccggctt
     1081 tggtcagcgc gttgaacagg gtagatttcc cgacgttggg caaaccgacg ataccgcatt
     1141 tgaatcccat gatttaaatc accttaatat cttaataatc aacctgttat tgctaacaga
     1201 ttgcagaaat ggaaataact ttgcctatta tacacggcac tcggcaaaaa tgccgcagac
     1261 aacgacttat tgcgctttaa aggcgtgcaa tcggttcgtt gctttggtca agccatctgt
     1321 aaaccatatt tcagtacaac gtgccgcttc gtcgatggct tcatcaatta acttctgttc
     1381 actaacaggc ggtttgccta acacaaaacc gacaacttta tttttatcgc ctggatgacc
     1441 aattccgatg cgtaaacggt gaaagttagg gttattaccc aatttactga tgatgtcttt
     1501 cagtccattg tgaccaccat ggccaccgcc caacttaaat ttggcgacgc caggaggcag
     1561 atccagttcg tcgtgggcca ccagaatctc gtccggatta atgcggaaaa aactggccat
     1621 cgccgcaacg gctttgccgc tgagattcat aaatgtagtc gggactaaca ggcggacatc
     1681 ttcgcctcca agggtgactc gcgaagtata accaaagaat ttagcctctt cgcgcagcgg
     1741 agcgcgcaat cgctctgcca gtaagtcaac gaaccaggca ccagcattat gtcgcgttgc
     1801 ggcgtattca gcgccggggt tcgccaggcc gacaatcaat ttaatcgtca cgtttttttg
     1861 tcctgagtgt gtacataact ggcgcgtagt ttactgtttg cggccccgct tgacaaaaaa
     1921 ctgcgtatca aatgcagata acgcaataat tgcctaagtg gactattaga aagtcaaggt
     1981 gttcaggcgt ttatttgtaa agttttgttg aaataagggt tgtaattgtg atcacgcccg
     2041 cacataaccc acagggtgtt gtctatactt tacacataag gaagaggggt attccctgtt
     2101 acaacccaga aagttccgga ggtgacatat gaaacgcaaa aacgcttcgt tactcggtaa
     2161 cgtgctcatg gggttgggtc tggtggtaat ggtggtcggc gtggggtatt caatcctcaa
     2221 ccagttacca cagtttaata tgccccagta tttcgcacat ggtgcagtgc taagtatttt
     2281 cgtcggtgcc attctctggc tggcgggtgc ccgtgttggc gggcatgaac aggtgtgcga
     2341 ccgttactgg tgggttcgcc actatgacaa acgttgccgc cgtagcgata atcgccgtca
     2401 tagctaacaa ataatgcggt ttgctgacca gtcagtttgc gctgactggt caatttcgta
     2461 cttataaatc cgccatcgcc gcgcgacgat tcgggaagaa cgccaggcgt cccgggatcg
     2521 gttgaatgcc tgcgcgagcc atagtgcgca gtggctggaa ttccacgttg cacacgcgca
     2581 gttcacatcc ttcgggtaga cgcttcacaa aacgctggaa cgcatcaaga ccaccagcat
     2641 caagtaccgg aacggcatcc cacttcagaa tcacaatccg tttgccttca agacgtgact
     2701 ccaggtccgt gaacaagcct tcagcggcag caaagaacag cgggccaata acgcgcagaa
     2761 ccagaacatc gtctggaacg tctacgacta ccggtgccag gcgagtcata cgtgcgatac
     2821 gacgcataaa cagtagcgat gccagcacga tccccacgct gatggcaata accatatcaa
     2881 acagcacggt cagcgacatg cacagcagca tgacgatgat gtcatctttc ggcgcatgac
     2941 gcagcaagtc gaccactttg tgcgcttcac tcatgttcca cgccaccatc aacagcaggg
     3001 cagccatggc ggaaagaggc agccaggaga gcagcggtgc cagtaccagc agggcaagaa
     3061 taaccagaat agagtggatc accgccgaga taggggaagt tgccccggca cggacgttag
     3121 cggcagaacg cgcgatggca gctgtagcgg taataccacc aaagaacgga gcgatgatat
     3181 tccccagtcc ctgtccaacc agttcactgt tcgctttgtg tttcgtcccg gtcataccat
     3241 ccagcaccac ggcgcagagc agtgattcaa ttgcccctaa catcgccatc gaaaatgctg
     3301 ccggaagcag agtgcgaatg gagtcccagg tgagggtaaa ttcggaattg ggcaggtccc
     3361 acggcagtac cagttgcggc agcagttgcg gaataccgtt accctgagaa ccatcggcca
     3421 gaacgtagtg gaattgcgaa ccgatggtag caacatgtcc gccgagcagg ttaacaatcc
     3481 ccatcaccgc gcaaccagcc agcaaggccg gaaggtgacc gggtaaacga atgcccagac
     3541 gtggccagaa aaccagaatg ccaagcgtca caatgccaat ggcagcatca cccacattaa
     3601 tggtcggcaa cgccataaat aatgcgccga ctttttgtag ataatgttca gggacatggg
     3661 ccatttgcag accgagaaaa tctttaatct gcatggtacc gatggtgatc ccgataccgg
     3721 aagtaaaacc taaggtaacg gaaaccggaa tatattcaat caagcgtcca aaacgtgcca
     3781 gccccatcag gatcaaaaag acacctgaca tgagtgtcgc gaccagcagt cctgccagtc
     3841 caaactgttg cgaaacggga tagagaatta ccacaaatgc cgcagtcgga ccggaaacgc
     3901 taaagcgtga cccacccgtc agagcaatga caatccccgc aacagctgcg gtatataaac
     3961 cgtactgggg tgccacacca ctaccaatag ccaacgccat tgccagcggg atagcaataa
     4021 tcccgacggt tatcccggca atcaggtcac gggtaaaccg tgcggcagta tatttttctt
     4081 tccagcaagc gtcgatcaga gcgcggaaag gcatcacatg tgaggaaaat attttgttca
     4141 caataatgtt tcatccgtga gcgcatcatc tgtcaactaa atggcaggtg aaggaggcat
     4201 aggtcataca aatggatatt acagacaaaa aaacccgccg cagcgggtct ttgagccggg
     4261 ttcgattagt gttcgaacat ggcagagatc gattcttcgt tgctgatacg acgaatcgct
     4321 tcggccagca tacctgacag ggtcagagta cgcacgttcg gcagtgattt gatttcatcg
     4381 ctcagcggaa tggtatcgca gacaacgact tcatcaatta cagagttacg caggttgttc
     4441 gccgcgttgc cagagaagat cgggtgagtc gcgtacgcaa atacacgttt agcaccacgt
     4501 tctttcagag cttcagcagc tttacacagc gtaccgccag tatcgatcat atcatcgacc
     4561 agtacgcagt cacgacctgc aacgtcaccg atgatatgca tcacctggga aacgttcgca
     4621 cgcggacgac gtttgtcgat gattgccata tcggtatcgt tcagcagctt agcgatagcg
     4681 cgggcacgca caacgccgcc gatgtccgga gaaaccacaa ttgggttatc cagattcagc
     4741 tgcagcatgt cttccagcag gatcgggcta ccaaatacgt tatcaaccgg aacgtcgaag
     4801 aaaccctgaa tctgttcagc gtgcagatcc actgtcagca cacggtcaac accgacgctg
     4861 gagaggaagt ctgcaaccac tttcgcagtg attggtacac gagcggaacg gacgcgacgg
     4921 tcctggcgcg catagccaaa gtaggggata acagcggtga tacgacctgc ggaagcacga
     4981 cgcagggcat caaccataac gactaattcc atcaggttgt cgttagtagg ggcacaagtg
     5041 gactggatga tgaaaatatc accaccgcgt acattttcgt taatttgtac gctgacttcg
     5101 ccgtcgctaa agcgacctac agcggcgtcg ccgagtgaag tgtacaggcg gttggcaata
     5161 cgttgtgcta gttccggggt ggcgttacca gcaaaaagct tcatatcagg cacgagaaga
     5221 acctcaggca tgcgtccatt ggtggaaaga atctgccgaa aactgtgcgg gccaggcatg
     5281 attctttcca ggcggtgtat taaagagcgc gatgcaacgt ctggaacaag gtgacgttgt
     5341 caccgaaact cagcttgccc ggcttaaagc atggctctgt gcaatgggga aagattaacg
     5401 cctttcgcca caaagccatt gagccattcc ggggcttgct ctagcacctg gcgggcttca
     5461 gactctgtat caaattcagc aaagacacag gcccctgtcc cagtcaggcg cgacggggcg
     5521 tattctaaca gccaggaaag caccgcatca acctcgcgaa aacgttttct tgcgataacc
     5581 tcgcaatcat tgctgaattc acattttagc aacgtttcta ttgacctttt tggcgtattg
     5641 cgcgggagtt caggatcttt aaaaatcacc ggtgtcggaa tacttacacc agggtgcgcc
     5701 accaagtacc acttctctgg cggatccacc ggcgttagta tttcaccaac gccttcggca
     5761 aacgcggcat gcccccgaac aaagacagga acatctgcgc ccagcgtcag ccccatttcc
     5821 gccagctcat ccatgcttag cccgcattgc cagagatggt ttaatgccac cagcaccgtc
     5881 gcggcattgg atgaaccacc acccagaccg ccgcccatcg gcaaacgctt gtcaatactg
     5941 atttctgcac cgcttcccgt aggaagacgc ccgctgtctg ccgctgtttt catcagcaac
     6001 cgtgctgcgc gaacgatcag gttatcttca tgttccacgc cttcaacggg tgttaacaga
     6061 cgaatatccc catcgtcacg aagctcaatg ctgatggtgt cgccgtaatc caggaactga
     6121 aacagcgttt gcagcgtgtg gtaaccgtcc gcacgctgac cggtaatgta taaaaacaga
     6181 ttaagttttg ccggagaggg ccactgtgtc cgcattattt cactatccag ttatccattt
     6241 ttaacttgat gcgctgacca ccatcggtga gttccatatt ggatggcatc gcaggttgag
     6301 tttttgtgtc ataaccaccg taaacaactt tccagttttt gccattctgg ctgtaggtga
     6361 tttcgctaag gcggtactgg tcgtccagtt tgtagtcagt cgcatcaccc ggcaaaccta
     6421 aaatccactg gcgtaagctg ttgagtggaa tgggcattcc ggtcaatttg ccaatcatct
     6481 cttcggcgtc atcggcggta taacgctggc ctttattgtc gactaactgc acgttacccg
     6541 gttgagcatt cagctccagt tccgtgctgc ccaatgggtt agtcagcagc agacggtagc
     6601 gatcctggcc agtttgctgc cagaagaaac gggcgtacac tttttgttgg tcagaaatat
     6661 aagcgaacgc gccgcgagtt tgatactgat taagattgcg cacgtcttgc tgatgctgac
     6721 gccattgtgg cgaatccggg cttttgccag gacctttggg cgtggtaacg gaacaggcag
     6781 tgagcacaag agcagccagc ggtagtaggc ggataagacg aaaatcgggc aggggcatag
     6841 tgatgacaag tccttgagat acgttgcagt tataaccctt aatgctagcg ttaccgtccg
     6901 ctatcgtcta tgttcaagtt gtcttaattg ccagaatcta acggctttcg gcaattactc
     6961 caaaaggggg cgctctcttt tattgatctt acgcatcctg tatgatgcaa gcagactaac
     7021 cctatcaacg ttggtattat ttcccgcaga catgaccctt ttagcactcg gtatcaacca
     7081 taaaacggca cctgtatcgc tgcgagaacg tgtatcgttt tcgccggata agctcgatca
     7141 ggcgcttgac agcctgcttg cgcagccgat ggtgcagggc ggcgtggtgc tgtcgacgtg
     7201 caatcgcacg gaactttatc ttagcgttga agagcaggat aacctgcaag aggcgttaat
     7261 ccgctggctt tgcgattatc acaatcttaa tgaagaagac ctgcgtaaaa gcctctactg
     7321 gcatcaggat aacgacgcgg ttagccattt aatgcgtgtt gccagcggcc tggattcact
     7381 ggttttgggg gagccgcaga tcctcggtca ggttaaaaaa gcgtttgccg attcgcaaaa
     7441 aggccatatg aaggccagcg aactggaacg catgtttcag aaatctttct ctgtagcgaa
     7501 acgtgttcgc actgaaacag atatcggtgc cagcgctgtt tctgtcgctt ttgcggcttg
     7561 tacgctggcg cggcagatct ttgaatcgct ctctacggtg acggtactgc tggtgggtgc
     7621 gggcgaaacc atcgagctgg tggcgcgtca tctgcgcgaa cacaaagtac agaagatgat
     7681 tatcgccaac cgcactcgcg aacgtgccca aattctggca gatgaagtcg gcgcggaagt
     7741 tattgccctg agtgatatcg acgaacgtct gcgcgaagcc gatatcatca tcagttccac
     7801 cgccagcccg ttaccgatta tcgggaaagg catggtggag cgcgcattaa aaagccgtcg
     7861 caaccaacca atgctgttgg tggatattgc cgttccgcgc gatgttgagc cggaagttgg
     7921 caaactggcg aatgcttatc tttatagcgt ggacgatctg caaagcatca tttcgcacaa
     7981 cctggcgcag cgtaaagccg cagcggttga ggcggaaact attgtcgctc aggaaaccag
     8041 cgaatttatg gcgtggctgc gtgcacaaag cgccagcgaa accattcgcg agtatcgcag
     8101 ccaggcagag cacgttcgcg atgagttaac tgccaaagcg ttagcggccc ttgagcaggg
     8161 cggtgacgct caagccatta tgcaggatct ggcatggaaa ctgactaacc gcctgatcca
     8221 tgcgccaacg aaatcacttc aacaggccgc ccgtgacggg gataacgaac gcctgaatat
     8281 tctgcgcgac ggcctcgggc tggagtagca gtacatcatt ttcttttttt acagggtgca
     8341 tttacgccta tgaagccttc tatcgttgcc aaactggaag ccctgcatga acgccatgaa
     8401 gaagttcagg cgttgctggg tgacgcgcaa actatcgccg accaggaacg ttttcgcgca
     8461 ttatcacgcg aatatgcgca gttaagtgat gtttcgcgct gttttaccga ctggcaacag
     8521 gttcaggaag atatcgaaac cgcacagatg atgctcgacg atcctgaaat gcgtgagatg
     8581 gcgcaggatg aactgcgcga agctaaagaa aaaagcgagc aactggaaca gcaattacag
     8641 gttctgttac taccaaaaga tcctgatgac gaacgtaacg ccttcctcga agtccgtgcc
     8701 ggaaccggcg gcgacgaagc ggcgctcttc gctggcgatc tgttccgtat gtacagccgt
     8761 tacgccgaag cccgccgctg gcgtgtagaa atcatgagcg ccagcgaggg tgaacatggt
     8821 ggttataaag agatcatcgc caaaattagc ggtgatggtg tatatggtcg tttgaaattc
     8881 gaatctggcg gtcatcgcgt gcagcgtgtg cctgctacgg aatcgcaggg tcgaattcat
     8941 acttccgctt gtaccgttgc ggtaatgcca gaactgcctg acgcagaact gccggacatc
     9001 aacccagcag atttgcgcat tgatactttc cgctcgtcag gggcgggtgg tcagcacgtt
     9061 aacaccaccg attcggcaat tcgtattact cacttgccga ccgggattgt tgttgaatgt
     9121 caggacgaac gttcacaaca taaaaacaaa gctaaagcac tttctgtact cggtgctcgc
     9181 atccacgctg cagaaatggc aaaacggcaa caggccgaag cgtctacccg tcgtaacctg
     9241 ctggggagtg gcgatcgcag cgaccgtaac cgtacttaca acttcccaca ggggcgcgtt
     9301 accgatcacc gcatcaacct gacgctctac cgcctggatg aagtgatgga aggtaagctg
     9361 gatatgctga ttgaaccgat tatccaggaa catcaggccg accaactggc ggcgttgtcc
     9421 gagcaggaat aatggaatat caacactggt tacgtgaagc aataagccaa cttcaggcga
     9481 gcgaaagccc gcggcgtgat gctgaaatcc tgctggagca tgttaccggc agagggcgta
     9541 cttttattct cgcctttggt gaaacgcagc tgactgacga acaatgtcag caacttgatg
     9601 cgctactgac gcgtcgtcgc gatggtgaac ccattgctca tttaaccggg gtgcgagaat
     9661 tctggtcgtt gccgttattt gtttcgccag cgaccttaat tccgcgcccg gatacggagt
     9721 gtctggtgga gcaggcactg gcgcggttgc ctgaacaacc ttgccgtatt ctcgatctcg
     9781 ggacgggtac cggggcgatt gcgctggcgc tggccagcga gcgtccggac tgcgaaatta
     9841 ccgctgtaga tcgtatgcca gatgctgtcg ccctggcgca acgtaatgcc cagaatctgg
     9901 cgatcaaaaa catccatatt ctgcaaagcg actggtttag cgcgctagcc gggcagcagt
     9961 ttgcgatgat tgtcagtaat ccgccgtata ttgacgagca ggacccacat cttcaacaag
    10021 gcgatgtccg ctttgagccg ctcactgcgc tggttgcggc agacagtgga atggcagaca
    10081 tcgtgcatat catcgaacag tcgcgtaacg cgctggtatc cggcggcttt ctgcttctgg
    10141 aacatggttg gcagcagggc gaagcggtgc gacaggcatt tatccacgcg ggatatcatg
    10201 acgtcgaaac ctgtcgggac tatggtgata acgagcgcgt aacgctcggc cgctattatc
    10261 aatgacaagt ttttctacac tgcttagtgt tcatcttatt agtatcgcgc tttcagttgg
    10321 gctattaacc ttacgtttct ggctacgtta tcagaagcat ccacaggcat ttgcgcgctg
    10381 gacgcgcatt gtgccgccgg ttgtcgatac gctgttattg ttaagcggca ttgcgttgat
    10441 ggctaaagcg cacatcctgc cattttccgg gcaggcacag tggctgactg aaaagctgtt
    10501 tggagttatc atttatatcg ttttgggttt tattgcactc gattatcgtc gtatgcacag
    10561 tcagcaggcg cgcattattg ccttcccgct ggcgttggtg gtgctgtaca tcatcattaa
    10621 actcgccacc acaaaagtac cgttactggg gtaagtcatg agatcgttag ctgatttcga
    10681 atttaataaa gcgccattgt gcgaaggcat gatcctggct tgcgaagcaa tccgccgcga
    10741 ttttccctcg caagatgttt acgacgaact ggagcgtctc gttagtctgg cgaaggaaga
    10801 aatcagccag cttctgcctt tagaagagca gctggaaaaa ctgatcgcgc tgttttacgg
    10861 cgactgggga tttaaagcct cacgcggcgt ttatcgtctt tccgatgcat tatggctgga
    10921 ccaggtgtta aagaatcgac agggcagtgc ggtatcatta ggtgccgttt tattatgggt
    10981 cgcgaatcgt cttgatttgc cgctgctgcc ggtgattttc cctacgcagc tgatattgcg
    11041 cattgaatgt ccggatggcg aaatttggct gattaatcct tttaacggtg aatcgttaag
    11101 cgaacatatg ctggacgtat ggttaaaggg aaatatcagc ccgtcggcgg aactgtttta
    11161 tgaagacctt gatgaagctg ataacattga ggtaatccgc aaattgctgg atacactcaa
    11221 agcttcgttg atggaagaga atcagatgga gctggcgtta cgcaccagcg aagctttatt
    11281 acaattcaac ccggaagatc cctatgaaat tcgcgatcgc gggttgattt atgcgcaact
    11341 ggattgcgaa cacgttgcgt tgaacgattt aagttatttc gttgaacagt gtccggaaga
    11401 cccgatcagc gaaatgatcc gtgcgcaaat aaataacatc gcgcataaac atattgtgct
    11461 gcattaatta atcgacattt tactcaagat taaggcgatc ctatgaaaca aaaagtggtt
    11521 agcattggcg acatcaacgt agcaaatgac ctgccgttcg tactgtttgg cggtatgaac
    11581 gtgctggaat ctcgcgatct ggcgatgcgc atttgcgagc actacgtaac cgtgacccag
    11641 aaactgggta tcccttacgt gttcaaagcc tcttttgaca aagccaaccg ctcctccatc
    11701 cactcttatc gtggaccggg cctggaagaa gggatgaaaa tcttccagga gttgaagcag
    11761 acttttggtg tgaaaattat caccgacgtt cacgaaccaa gtcaggcaca gcccgttgcg
    11821 gatgtcgtgg atgtgattca gttgccggcg tttcttgctc gccagactga cctggtggaa
    11881 gccatggcga aaaccggtgc ggtaattaac gtcaagaaac cgcagtttgt tagcccggga
    11941 cagatgggta atatcgttga taaatttaaa gaaggcggca acgaaaaagt gattctttgc
    12001 gatcgcggtg ctaacttcgg ctatgacaac ctggttgtcg atatgctggg cttcagcatc
    12061 atgaagaaag tgtctggtaa ttcgccggtg attttcgacg tgacccacgc actgcaatgc
    12121 cgcgatccgt ttggcgcagc ttccggcggt cgtcgtgctc aggtggctga gctggcacgt
    12181 gccggtatgg cggtaggtct ggcggggctg tttattgaag cgcatccgga tccggaacat
    12241 gcgaaatgtg atggtccatc cgcgctgccg ctggctaaac tggaaccgtt cctcaagcag
    12301 atgaaagcga ttgatgactt ggtgaaaggt ttcgaagaac tggataccag caagtaatct
    12361 tttttgcctg aaaactaaag ccttagcgtt ctgcgttaag gcttttttca tgggggccgg
    12421 atacaaaaag ggccgcaggc tgttacccct gcggccggtt tcgggcgcat attgccatca
    12481 cggcagcctg acgcccgttt tcgccttact tccggttacg ccaccagccg acaatcgctg
    12541 cggtaataat tcccgccagg atcggtgctg ccaggtcgtg ccagaaaatc acggtaaact
    12601 gcgcgagcgt catatagccg ccttgttgta atgacaacat tttgcggcta ttcttgaatt
    12661 gttctggttc aagattagcc cccgttctgt tgtcaggtta tacctctcaa cgtgcggggg
    12721 ttttctcttt ccagcaacca atgccaccag ggataaagcc cccgcaacat tgcgcctcac
    12781 cggataacgc cggcttggtg tggatactac gactcaattc atcttcactt catccctgaa
    12841 atgtttgcaa tgaagagtgc attccggttt ttcaacagct gttacagtcg tttcgtgcag
    12901 gttgtggttc aggctcgcaa ttctgattaa caacctgtcc aggtagtatg aagaaataca
    12961 ataagcagga atttatatta tctcccccga tttccgtcat cggaggagat ataactgtca
    13021 ggcaaatatc gtcatcaaat aggcggcaaa cagtgccaga tgcgctgcgc cattgagcac
    13081 gttagtacgt ccggtggaga aggagatatg acacagcact aaagaggcca ccatcaccac
    13141 catttctggc gcaccaagtg caaactgcaa ttcgttaccc gtcataaagg caattagcgt
    13201 gacgacaggt acggtaagcg aaatggttgc taacactgaa ccaaagaaca gattcatcgc
    13261 gcgctggacc tggttgttca acactgcttt taacgcacct aaaccttccg gcgacagaat
    13321 caacagagcc accaggaagc cagtaaaggc gacaggggca ttcatgctgt cgagcaatgt
    13381 ctccagcggg ctggcgttca ttttggtcac cgcaataacg gcaatcagat ggacaatcaa
    13441 ccagatagca tgccacaggc tgctatgggc agacggttta ccgtgatgcg ggtcgtcgtc
    13501 atcactgtca tcttcgtgct cgtagacaaa caaactttga tgcgttttgg tctggatcag
    13561 caaaaatacg ccatacattg ccgcagaaat taatgctacc agtaacgcct gaccggttga
    13621 aaaattcgcc gcaggcagag ccattggaaa taccagtacg attatcgcca gggggaacag
    13681 cgcaattaaa tactgcttga taccaaacag attcatatat tgggtggcaa acttgcgtcc
    13741 gcccaacaat aatgaaaagc caaccagccc accggtaaca atcataatga ttgaatagag
    13801 cgtatcacgc attagcgttg gcgcggcgtc gccggttgcc attaaagctg aaatcaaact
    13861 gacttcaaga ataaccactg aaaggctaag aataagcgaa ccgtaaggct ctcccaggcg
    13921 atgggctaat acgtccgcat gacggacaac actaaaggcg ctacttaaaa taccaataag
    13981 agcaagaaga ttgatggcaa tgaccactgg tagtgtctgg ctgcttcccc acaggaacag
    14041 cactaccagc gccagaaccg ggaaaataag cgaagtctcc ttgtggcggg tttttaccgc
    14101 ctcttgagca tttgacatta tggttatccc tttgcagatg aatttatcga aaatgtaaaa
    14161 aataggtagg aaaaataaca gaaagtgtct ggatatcggt aacattttac gaatttttac
    14221 cccgctgtcg attttttact catttgggca taaaaataag taatacgttt agacaatgtt
    14281 tgttttagca cttcttaaga agagtctgac atgaaaattc ttatgttttg gcaagagtag
    14341 atattgttga ccacacttaa tgttcaactt tgtaaaagga gtcaacgatg ccgtataaaa
    14401 cgaaaagcga tctgccggaa agcgtaaagc acgtgcttcc gtctcatgcc caggatatct
    14461 ataaagaagc gttcaacagc gcatgggatc aatataaaga taaagaagat cggcgtgatg
    14521 acgccagtcg cgaagaaaca gcgcataaag tggcctgggc tgctgtgaag catgaatatg
    14581 ccaaagggga tgatgataaa tggcataaaa aatcgtaaaa ccggtagctt agttaaagct
    14641 attcgtgcgg tgttgccttg caagtggtcc gtggattgca tattgtcccg ttagtggttt
    14701 caaaatgagc agtaaaaatg tccggaagac accaaaaagt tgtcgcaggg aagtatgcag
    14761 tggcggaggt gtaaggtgat aacgcgtgat ttcttgatga atgccgattg taaaacggca
    14821 tttggtgcca ttgaagaatc actcttatgg tcagcagaac aacgggcggc ttcgctggcg
    14881 gcgacgctgg cttgtcgacc tgatgaggga ccggtgtgga tcttcggtta tggatcgttg
    14941 atgtggaatc cggcactgga gtttaccgag tcgtgcaccg gtacactggt tggatggcat
    15001 cgtgcgtttt gcctgcgcct gaccgccggg cgcgggactg cgcaccagcc gggacggatg
    15061 cttgcactga aagagggcgg acgcaccaca ggcgtcgcct atcgactgcc agaagagacg
    15121 ctggagcagg aactaaccct gttgtggaag cgcgagatga ttaccggctg ttatctgcca
    15181 acctggtgtc agcttgatct tgatgatgga cgcacagtaa acgccattgt gtttattatg
    15241 gacccgcgac atccagaata tgaatctgac actcgcgctc aggtcatcgc gccgttgatt
    15301 gcggcggcga gcggtccact gggaaccaac gcacaatacc tgttttcact ggaacaggag
    15361 cttatcaaac tgggaatgca ggacgatggg ctgaatgatt tgctggtatc ggtaaaaaaa
    15421 ctgctggcgg agaattatcc ggatggtgtg ttacggccgg gactcgcctg agtaaacttc
    15481 cctcatagtg gggcgtcaga cgccccctca aacattaaaa tgtgagcact ttatcggctg
    15541 acagcgtcca ttgcgccagt tccacaagcg taccgatttc caccccatca atcagaggaa
    15601 gtgtactaat cccgcgcccg tcggtacagg ttttgcacaa tttcaccggt acattctgag
    15661 cggtaaggat ctccagcatt tgctgaatgt tgtagccttc ccctggtttt tgcccgcgca
    15721 acccggctgt gaccgcatca gacatcagga acagacgcag atccagattg ctctcctgct
    15781 ctcgtaacgc aatggccagc cgcaagctgt taaataagga ttcgctcccg taaggtgcgc
    15841 cattggcaac gatcacgatt ttttgcatta tttactcctg tattcaggga attagacact
    15901 catcttctat cttactgctt ctgcagcgtc tgaccaatcg gtcacatttt taaggatttt
    15961 cctgaaagcg cgagaaaata cgacaaaagt tgccagtaat cgttattctt tagggctatg
    16021 gtttttcatt ttttaccgga agttaccgac gttttgagcc gtttcgttcc tcgcattatt
    16081 ccgttttatt tactcttgct ggcggcaggc ggtacagcta acgcacaatc taccttcgag
    16141 caaaaagcgg caaatccctt tgataataac aatgatggtc tgccggattt aggcatggca
    16201 cctgaaaatc atgatgggga aaaacacttt gctgaaattg tgaaagattt cggcgaaacc
    16261 agtatgaatg ataacgggct ggatactggc gagcaggcaa aagctttcgc attgggaaaa
    16321 gtccgcgacg cgtttagtca acaggttaat cagcacgtag agtcgtggct atcagcgtgg
    16381 ggaaatgcca gtgttgacgt caaagtggat aacgaaggac atttcaccgg cagtcgtgga
    16441 agctggtttg tgccgttaca agataatgat cgttatctca cctggagcca gcttggtctt
    16501 actcagcagg atgatgggct ggtgagcaat gtgggcgttg ggcaacgctg ggcgcgcggc
    16561 agctggctgg tgggttataa cactttttat gacaacttgc tggacgaaaa tcttcagcga
    16621 gcgggcttcg gtgccgaagc gtggggcgaa tatttgcgac tatcggcaaa cttttatcag
    16681 ccgtttgctg catggcatga acagacagcc acgcaggaac aacggatggc gcgcgggtac
    16741 gacctgacag cccggatgcg catgccgttt tatcaacacc tcaataccag tgtcagcgta
    16801 gaacagtatt ttggtgatcg tgttgatttg tttaactctg gtacgggtta tcacaatccc
    16861 gtcgcgttga gtctgggatt aaattatacc ccagtgccat tagtcactgt gacggcccag
    16921 cataaacagg gtgaaagtgg cgaaaatcaa aataacctcg ggctgaatct taattaccgc
    16981 tttggtgtac cgctcaaaaa acaactttct gcgggcgagg ttgccgaaag tcagtcgtta
    17041 cgtggtagtc gctatgacaa tccgcagcga aataatctac cgactcttga gtaccgacag
    17101 cgaaaaacgt taacggtgtt tctggcgaca ccgccgtggg atctaaaacc tggcgaaaca
    17161 gtgccgctga aattacaaat ccgcagtcgt tacggtattc ggcaactgat ttggcagggc
    17221 gatacgcaga tattaagttt gacgccgggc gcacaagcca acagtgagga gggctggacg
    17281 ctgatcatgc ctgactggca aaacggggaa ggcgcaagca atcactggcg attgtcagtg
    17341 gtggtggaag ataaccaggg gcagcgtgtc tcctccaatg agatcacgct aacgcttgtc
    17401 gaaccgttcg acgcattgtc aaacgacgaa ctgcgctggg aaccgtaatc agaaaatgcg
    17461 ctcctgatgc acccataccg cagcttccac gcgagacttt agcttcattt tcttcagcat
    17521 gtgcttgacg tgaactttta ctgtgctttc ggtgatgtcc aggcggcggg caatcatctt
    17581 gttcggcaaa ccctgggcaa ttagcttgag aatatcgcgc tcgcgtgggg ttaactggtt
    17641 aacatcgcgc tcagtagtgg cacggttagc gcgcaagctg gcggccagaa caggcgttaa
    17701 tgcttcgctt aataccattt cgccagcagc tgcctgatgt aacgctttca gcagatcttc
    17761 cggttccata tcttttaaca gatagccatc cgcgccgcgt ttcagtgcgg tgaccacatc
    17821 ttcttcatgg ttagagacgc tgaataccac aatgcgcccg gagagggact tttcgcgcag
    17881 tttatccagc gtttccagac cgttcatgcc gggcatattg agatctaaca ggatcagatc
    17941 gggatcaaga gactccgcca gttcaatacc ctgttcgcca ttactcgctt cgccaaccac
    18001 ggtgatatct ggtgccatac tgataagctg ttttacgcca gttcgcagca tcgggtgatc
    18061 gtcaatcagc aggatagtag ccggttcctg attactcatg ggtatctcct tggacgtctg
    18121 tgaaagtttt ttcgg
//
LOCUS       DAZFLD010000094        18051 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.094, whole genome shotgun sequence.
ACCESSION   DAZFLD010000094 DAZFLD010000000
VERSION     DAZFLD010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18051
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.094"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            150..716
                     /locus_tag="ACHKBL_002690"
     CDS             150..716
                     /locus_tag="ACHKBL_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000033313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO5819543.1"
                     /translation="MSKFVKAAVATAMLMGALTSASVVAAGNNGTVRFYGTIEDSPCS
                     IVPDDHKLEVDMGSIGTGSLTGGKTTTPKDFQIRLQDCNFNTETTMTTTFTGNPYSGN
                     SKNYSLSNMDNGTEIPNVSLVIGDQRGTGYDLGAGIDQDIAMDSSTGKGKQKQTLNFK
                     AWLVGETAAVTPTPAPFETLTTFQITYL"
     gene            802..3441
                     /locus_tag="ACHKBL_002691"
     CDS             802..3441
                     /locus_tag="ACHKBL_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014639959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HFO5819544.1"
                     /translation="MPEHSFLRLRGLSWWIALAISGSPFNVLADDTIQFDARFLELKG
                     NTKIDLGRFSQKGYVEPGKYNLRVHVNNHPLPDEYDIYWYVAENDPNKSYACLPPELI
                     AQFGFKDDFAKSLQWGHDGQCLKTDQIGGMEIKGDLSQSALLVSVPQAYLEYTDDDWD
                     PPSRWDEGIPGLIADYSINAQTRHENGGDDTNDISGNGTVGVNVGPWRLRADWQSDYQ
                     HTRSNDDGDTDDSGTQKNWEWSRYYAWRALPSLKAKLSLGEDYLNSDIFDGFSYIGGS
                     ISTDDQMLPPNMRGYAPDISGVARTTAKVTVTQMGRVIYETQVPAGPFRIQDIGDSVS
                     GTLHVRIEEQNGQVQEYDVSTASMPFLTRPGQVRYKVTMGRPQNWDHQVAGSFFSGGE
                     ASWGIANGWSLYGGALADENYQSAALGLGRDLALFGALAFDVTHSRVQLDDNSVYGNK
                     TLDGNSYRVSYAKDFDELNSRVTFAGYRFSEKNYMTMSEYLDANDTDRARTGNDKEMY
                     TVTYNQNFRDARVSVYLNYSHHTYWDREDQTNYNMMLSHYFNLGSLRNLSVSLTGYRY
                     EYDKSADKGVYLSLSLPWGDNSTISYNGNYGSGADSSQASLYHRIDDASHYTLSAGTS
                     ENHTSLDGYYSHDSTLAKVDLSANYHEGQYTSAGVSLQGGVTLTAHGGALHRTQNMGG
                     TRLLIDADGVANVPVEGNGSAVYTNMFGKAVVADVNDYYRNQAYIDLNKLPENAEATQ
                     SVVQATLTEGAIGYRKFAVISGEKAMVVLRLQDGSHPPFGAEVKNDNQQQVGLVDDEG
                     NVYLAGVKPGEHMTVFWEGESHCDISLPDPLPNDLFNGLLLPCQQKGGSAPVITHDIQ
                     PVIQEQTQQVTPMEPPMSVSSNQ"
     gene            3461..4210
                     /locus_tag="ACHKBL_002692"
     CDS             3461..4210
                     /locus_tag="ACHKBL_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFO5819545.1"
                     /translation="MTKHLSVKTLATVLSLLAATVSFSALASVTPDRTRLIFNESDKS
                     ISVTLRNNDPKQPCLAQSWIEDEKGVKISSPLAVLPPVQRIDAMMNGQVKIQGLPDIH
                     KLPADRESIFYFNVREIPPKSNKANTLQIALQTRIKLFWRPKALEKASMQNPWQHKVT
                     LTRNGQEFTVNNPTPYYVIFSNASAQKNGNPADGFSPIVMSPKTSAPLKVKMASVPVL
                     TYVNDYGARMPLFFTCSGNSCQVDEELSRKG"
     gene            4227..4733
                     /locus_tag="ACHKBL_002693"
     CDS             4227..4733
                     /locus_tag="ACHKBL_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO5819546.1"
                     /translation="MKRSCSVWAGTWLLMCCIGSDFAVAGSKSVNLSLRVLVDAPPPC
                     TVNGATVEFGNVFINKIDGVGYKQPIDYSLVCNNLAMDDLRLQMRATTVVINGETVIS
                     TGISGFGIRVQKSSDHSTLDLTSGSWLPFNFSSGVPALEAVPVKQSGTTLSAAEFNAS
                     ATIVVDYQ"
     gene            4730..5200
                     /locus_tag="ACHKBL_002694"
     CDS             4730..5200
                     /locus_tag="ACHKBL_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO5819547.1"
                     /translation="MKRISLLMLWGFSSMALSNVSFHGYLVQPPNCTISNGKTIEITF
                     QDVFVDDINGSNYEQTVPYSITCDTAVRDPSMEMKLSWSGTQSDFDDAAVSTDISGLA
                     IRLKQAGQSFKLNTALVVNETALPVLTAVPVKKSGVDLPEADFEAWATLQVDYQ"
     gene            5197..5721
                     /locus_tag="ACHKBL_002695"
     CDS             5197..5721
                     /locus_tag="ACHKBL_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO5819548.1"
                     /translation="MKKKRALVFICSLMLLYSVTAPAVDNLHFTGNLLGKSCTPVING
                     SLLAEVHFPTIAASDLMNLGQSERVPLVFQLKDCKSSTLFSVRVTLTGTEDSELPGFL
                     AFDASSSASGAGIGIETAAGAAVPVNSTTGVTFPLNQGNNSLNFNAWLQAKGGRDVTS
                     GDFSATATATFEYF"
     gene            5723..6565
                     /locus_tag="ACHKBL_002696"
     CDS             5723..6565
                     /locus_tag="ACHKBL_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001317961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="StfH/YfcO family fimbrial adhesin"
                     /protein_id="HFO5819549.1"
                     /translation="MKKWTMIFASLVLLVMSASVPKSYAGDKLTSASFDSVKIYYGKG
                     KMSIHNTGGLYVTVLTPNEAAYGVYNDAIRKNEQASVISWTGSGPAPVLILTDYGNAD
                     SSNCPGINTATFQCAYETFKITVQSDSYGCPWLASFYTITDQPGFGRYTGPTNHATVC
                     PTIPVASYDISWSENYVSHNKALQLQSNGSTITTTLSTYLMEDGKLCDGSVFDSRGAY
                     CRVVSELLTFTSYGCDKSTVTVSPTRHPVTDKQLHDIVVNVNTSSRQPIDSTCRFQYV
                     LNEL"
     gene            complement(6681..7232)
                     /gene="smrB"
                     /locus_tag="ACHKBL_002697"
     CDS             complement(6681..7232)
                     /gene="smrB"
                     /locus_tag="ACHKBL_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HFO5819550.1"
                     /translation="MKKKTTLSEEDQALFRQLMAGTRKIKQDTIVHRPQRKKISEVPV
                     KRLIQEQADASHYFSDEFQPLLNTEGPVKYVRPDVSHFEAKKLRRGDYSPELFLDLHG
                     LTQLQAKQELGALIAACRREHVFCACVMHGHGKHILKQQTPLWLAQHPHVMAFHQAPK
                     EYGGDAALLVLIEVEEWLPPELP"
     gene            7398..8330
                     /gene="prmB"
                     /locus_tag="ACHKBL_002698"
     CDS             7398..8330
                     /gene="prmB"
                     /locus_tag="ACHKBL_002698"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA];
                     GO_process: GO:0042273 - ribosomal large subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFO5819551.1"
                     /translation="MDKIFVDEAVNELQTIQDMLRWSVSRFSAANIWYGHGTDNPWDE
                     AVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERVIRRVNERIPVAYLTNKAWFCGH
                     EFYVDERVLVPRSPIGELINNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPEAEVD
                     AVDISPDALAVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSD
                     LPNEYRHEPELGLASGTDGLKLTRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDV
                     PFTWLEFDNGGDGVFMLTKEQLIAAREHFAIYKD"
     gene            8365..9450
                     /gene="aroC"
                     /locus_tag="ACHKBL_002699"
     CDS             8365..9450
                     /gene="aroC"
                     /locus_tag="ACHKBL_002699"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HFO5819552.1"
                     /translation="MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGIPLTEADLQHD
                     LDRRRPGTSRYTTQRREPDQVKILSGVFEGVTTGTSIGLLIENTDQRSQDYSAIKDVF
                     RPGHADYTYEQKYGLRDYRGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQM
                     GDIPLEIKDWSQVEQNPFFCPDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGL
                     GEPVFDRLDADIAHALMSINAVKGVEIGDGFDVVALRGSQNRDEITKDGFQSNHAGGI
                     LGGISSGQQIIAHMALKPTSSITVPGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEA
                     MLAIVLMDHLLRQRAQNADVKTDIPRW"
     gene            9453..10277
                     /gene="mepA"
                     /locus_tag="ACHKBL_002700"
     CDS             9453..10277
                     /gene="mepA"
                     /locus_tag="ACHKBL_002700"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HFO5819553.1"
                     /translation="MNKTAIVLLALLASSASLAATPWQKITQPVPGSAQSIGSFSNGC
                     IVGADTLPIQSEHYQVMRTDQRRYFGHPDLVMFIQRLSRQVSNLGMGTVLIGDMGMPA
                     GGRFNGGHASHQTGLDVDIFLQLPKTRWTSAQLLRPQALDLVSRDGKHVVPTLWKPEI
                     FSLIKLAAQDKDVTRIFVNPAIKQQLCLDAGTDRDWLRKVRPWFQHRAHMHVRLRCPA
                     DSLECEDQPLPPPGDGCGAELQSWFEPPKPGTTKPEKKTPPPLPPSCQALLDEHVI"
     gene            10277..11086
                     /locus_tag="ACHKBL_002701"
     CDS             10277..11086
                     /locus_tag="ACHKBL_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFO5819554.1"
                     /translation="METFNSLFMVSPLLLGVLFFVAMLAGFIDSIAGGGGLLTIPALM
                     AAGMSPANALATNKLQACGGSISATIYFIRRKVVSLSDQKLNIAMTFVGSMSGALLVQ
                     YVQADVLRQILPILVICIGLYFLLMPKLGEEDRQRRMYGLPFALVAGGCVGFYDGFFG
                     PAAGSFYALAFVTLCGFNLAKATAHAKLLNATSNIGGLLLFILGGKVIWATGFVMLVG
                     QFLGARMGSRLVLSKGQKLIRPMIVIVSAVMSAKLLYDSHGQEILHWLGMN"
     gene            11086..11634
                     /gene="epmC"
                     /locus_tag="ACHKBL_002702"
     CDS             11086..11634
                     /gene="epmC"
                     /locus_tag="ACHKBL_002702"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HFO5819555.1"
                     /translation="MNSTHHYEQLIEIFNSCFADEFNTRLIKGDDEPIYLPADAEVPY
                     NRIVFAHGFYASAIHEISHWCIAGKARREQVDFGYWYCPDGRDAQTQSQFEDVEVKPQ
                     ALDWLFCVAAGYPFNVSCDNLEGDFEPDRVVFQRRVHAQVMDYLANGIPERPARFIKA
                     LQNYYHTPELTAEQFPWPEALN"
     gene            11668..11946
                     /locus_tag="ACHKBL_002703"
     CDS             11668..11946
                     /locus_tag="ACHKBL_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcL family protein"
                     /protein_id="HFO5819556.1"
                     /translation="MIAEFESRILALIDGMVDHASDDELFASGYLRGHLTLAIAELES
                     GDDHSAQAVHTTVSQSLEKAIGAGELSPRDQALVTDMWENLFQKASQQ"
     gene            complement(12002..14008)
                     /gene="mnmC"
                     /locus_tag="ACHKBL_002704"
     CDS             complement(12002..14008)
                     /gene="mnmC"
                     /locus_tag="ACHKBL_002704"
                     /EC_number="1.5.-.-"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)(34)-
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HFO5819557.1"
                     /translation="MKHYAIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLG
                     GNQLEARFPEHPHPLFVVAESGFGTGLNFLTLWQAFDQFREAHPQAQLQRLHFISFEK
                     FPLTRADLALAHLHWPELAPWAEQLQAQWPMPLPGCHRLLLDEGHVTLDLWFGDINEL
                     TSQLDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNAMARLARPGSTLATFTSAGFVRR
                     GLQDAGFTMQKRKGFGRKREMLCGVMEQTLPLPCSTPWFNRTGSSKREVAIIGGGIAS
                     ALLSLALLRRGWQVTLYCADEAPALGASGNRQGALYPLLSKHDEALNRFFSNAFTFAR
                     RLYDLLPVKFDHDWCGVTQLGWDEKSQHKIAQMLSMDLPTELAVAVGANEVEGVTGVV
                     TNCGGITYPQGGWLCPAELTCNVLKLAQQQGLQIRYQHQLKDLSRKDDCWLLNFAGDQ
                     QATHSVVVLANGHQISRFSQTSSLPVYSVAGQVSHIPTTPELAELKQVLCYDGYLTPQ
                     NPANQHHCIGASYHRGSEDTAYSEDDQQQNRQRLIDCFPQAQWAKEVDVSEKEARCGV
                     RCATRDHLPMVGNVPDYDVTLVEYASLAEQKDEAVSAPIYDDLFMLAALGSRGLCSAP
                     LCAEILAAQMSNEPIPMDASTLAALNPNRLWVRKLLKGKAVKAG"
     gene            14167..15387
                     /gene="fabB"
                     /locus_tag="ACHKBL_002705"
     CDS             14167..15387
                     /gene="fabB"
                     /locus_tag="ACHKBL_002705"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HFO5819558.1"
                     /translation="MKRAVITGLGIVSSIGNNQQEVLASLREGRSGITFSQELKDSGM
                     RSHVWGNVKLDTTGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVGLI
                     AGSGGGSPRFQVFGADAMRGPRGLKAVGPYVVTKAMASGVSACLATPFKIHGVNYSIS
                     SACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACEFDAMGALSTKYNDTPEKA
                     SRTYDAHRDGFVIAGGGGMVVVEELEHALARGAHIYAEIVGYGATSDGADMVAPSGEG
                     AVRCMKMAMHGVDTPIDYLNSHGTSTPVGDVKELAAIREVFGDKSPAISATKAMTGHS
                     LGAAGVQEAIYSLLMLEHGFIAPSINIEELDEQAAGLNIVTETTDRELTTVMSNSFGF
                     GGTNATLVMRKLKD"
     gene            15652..16830
                     /locus_tag="ACHKBL_002706"
     CDS             15652..16830
                     /locus_tag="ACHKBL_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO5819559.1"
                     /translation="MTAVSQTETRSSANFSLFRIAFAVFLTYMTVGLPLPVIPLFVHH
                     ELGYGNTMVGIAVGIQFLATVLTRGYAGRLADQYGAKRSALQGMLACGLAGGALLLAA
                     ILPVPAPFKFALLVVGRLILGFGESQLLTGALTWGLGIVGPKHSGKVMSWNGMAIYGA
                     LAVGAPLGLLIHSHYGFAALALTTMALPLLAWACNGTVRKVPALAGERPSLWSVVGLI
                     WKPGLGLALQGVGFAVIGTFVSLYFASKGWAMAGFTLTAFGGAFVVMRVMFGWMPDRF
                     GGVKVAIVSLLVETVGLLLLWQAPGAWVALAGAALTGAGCSLIFPALGVEVVKRVPSH
                     VRGTALGGYAAFQDIALGVSGPLAGMLATTFGYSSVFLAGAISAVLGIIVTILSFRRG
                     "
     gene            complement(16827..17822)
                     /gene="flk"
                     /locus_tag="ACHKBL_002707"
     CDS             complement(16827..17822)
                     /gene="flk"
                     /locus_tag="ACHKBL_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0010468 - regulation of gene expression
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulator Flk"
                     /protein_id="HFO5819560.1"
                     /translation="MIQPISGPPPGQPPGQGDNLPSGAGNQPLSSQQRTSLESLMTKV
                     TSLTQQQRAELWAGIRHDIGLSGDSPLLSRHFPAAEHNLAQRLLAAQKSHSARQLLAQ
                     LGEYLRLGNNRQAVTDYIRHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQREAT
                     DRPLLPAEHNALKQLVTKLAAATGEPSKQIWQSMLELSGVKDGELIPAKLFNHLVTWL
                     QARQTLSQQNTPTLESLQMALKQPLDASELAALSAYIQQKYGLSAQSSLSSAQAEDIL
                     NQLYQRRVKGIDPRDMQPLLNPFPPMMDTLQNMATRPALWILLVAIILMLVWLVR"
BASE COUNT         4321 a         4515 c         4776 g         4439 t
ORIGIN      
        1 ccaaagaata ttatatcaag gaacctgttt tttcaggagc tgaaaatctg ttcattggct
       61 gcttaaaagt cgctgcaaaa attgaagaag ttctgtgtca atatttcagt tggcttatat
      121 tctaattatt tatgatttaa ggacaaataa tgagtaagtt tgtaaaagca gcagttgcca
      181 cggcaatgct catgggggcg ctgacttcag catcagtcgt tgccgcaggt aacaacggta
      241 cagtgagatt ttacggcaca attgaagact ctccgtgctc gattgttcct gacgatcata
      301 aactggaagt ggatatgggc agcattggta caggttcttt aaccggtggc aagactacca
      361 cacctaaaga tttccagatt cgtttacagg attgtaattt caatactgaa acgacaatga
      421 ccacgacatt cactggtaat ccgtactccg gcaactcaaa aaactattcc ctgtctaaca
      481 tggataatgg aactgaaatt ccaaatgtaa gtctggtgat tggcgaccaa cgtggtactg
      541 gttatgacct gggggcaggc atcgatcagg acatcgcgat ggacagttca acaggaaaag
      601 gcaaacagaa acaaacgttg aactttaaag cctggttagt aggtgaaact gcagcggtaa
      661 caccaacccc agcgccgttt gaaacgctaa cgacgttcca gattacatat ctctaattat
      721 gctgttcccc cataactgca atgttatggg gtttataaat aaaagataac taaattataa
      781 taatcatgga tggatacgta gatgcctgaa cattcttttc ttcgtttacg ggggctttca
      841 tggtggattg ctctggcaat ttccgggagt ccttttaatg tactggcaga tgataccatt
      901 caatttgatg ctcgatttct ggagttaaaa ggaaatacca aaattgatct ggggcgtttt
      961 tctcaaaaag ggtatgttga accagggaaa tacaatttac gtgttcatgt aaataatcac
     1021 ccattacctg atgaatacga tatctattgg tatgtagctg aaaacgatcc gaataaatca
     1081 tatgcctgct taccacctga actgatagcc cagtttggct tcaaagatga ttttgcgaag
     1141 agtttgcaat ggggtcatga tggacaatgt ctgaaaactg accaaatagg aggtatggaa
     1201 attaagggcg atttaagtca atctgcgctc ttagtttctg tgccacaggc atatctcgaa
     1261 tacactgacg atgactggga cccaccttcg cgttgggatg aggggattcc tgggcttatt
     1321 gccgattaca gtatcaacgc acaaacccga catgaaaatg ggggggatga cactaacgac
     1381 atcagcggta atggtacggt cggcgttaac gtggggccgt ggcgcttacg agccgactgg
     1441 cagagcgatt accaacatac ccgcagtaac gatgacggcg acactgatga tagcggtacg
     1501 caaaaaaact gggagtggag ccgttactac gcctggcgcg ccttgccgtc attaaaagcc
     1561 aaactttcgc tgggtgaaga ttatctcaac tccgatattt ttgatggctt cagttacatt
     1621 ggcggcagca tcagtaccga tgatcaaatg ttaccgccaa acatgcgcgg ctacgcaccc
     1681 gatatttcag gtgttgcacg cactaccgct aaagtgaccg taacccaaat gggccgtgtc
     1741 atttacgaaa cccaagtgcc cgcaggtcca ttccgcattc aggatattgg cgattccgta
     1801 tctggcacgc tgcatgtccg cattgaagaa caaaacggtc aggtacagga atatgacgtc
     1861 agcacggcgt ccatgccgtt tctgacccgc cccggtcagg tgcgttacaa agtgacaatg
     1921 gggcgtccac aaaactggga ccatcaggtt gcaggcagct tcttctcggg cggtgaggcg
     1981 tcatggggga ttgcgaatgg ctggtcgctc tacggcgggg cgttggccga tgaaaactac
     2041 cagtcggcag cgttaggcct cggtcgtgac ctcgcgctgt tcggagcgct agcgtttgat
     2101 gtcactcact ctcgtgttca actcgacgat aacagcgtgt atggcaacaa aacgctggac
     2161 ggcaactctt atcgcgtcag ctatgccaaa gatttcgatg aactcaacag tcgggtgacg
     2221 tttgccggat atcgcttctc tgaaaaaaac tacatgacca tgagtgagta tctggatgcg
     2281 aacgacaccg accgtgcgcg cacgggtaac gacaaggaaa tgtacacggt cacttacaac
     2341 cagaacttta gggatgcgcg cgtttcggtc tatctcaatt attctcacca tacctattgg
     2401 gatcgcgagg atcagaccaa ctacaacatg atgctttctc attatttcaa tttaggaagt
     2461 cttcgcaacc tgagcgtttc tctcaccggt tatcgctacg aatatgataa aagtgccgac
     2521 aaaggtgttt atctgtcctt gagtttgcca tggggcgaca acagcaccat cagttacaac
     2581 ggcaattatg gcagtggtgc cgacagcagc caggcgagtt tgtatcaccg catcgatgac
     2641 gctagccact atacgttgag cgccggaacc agtgaaaatc acaccagtct tgatggttat
     2701 tacagtcatg acagtacgct ggcaaaagtt gacctgagtg caaactacca tgaggggcaa
     2761 tacacatctg cgggggtctc cttacagggc ggtgtaacac tgactgctca cggcggggcg
     2821 ttgcatcgta cgcaaaatat gggaggcaca cgtctgctaa tcgacgccga tggcgttgct
     2881 aatgtgccgg tcgaaggtaa tggttccgcc gtttatacca acatgttcgg gaaagcggtg
     2941 gtggcagatg ttaatgatta ctaccgtaac caggcttata tcgatttgaa caaactaccg
     3001 gaaaacgccg aagctaccca atcggtggtc caggcaacgc tgacggaagg cgctattggc
     3061 taccgtaaat ttgcggtgat cagcggtgag aaggcgatgg tagtgctgcg cttgcaggat
     3121 ggcagccatc cgccatttgg ggcggaagtg aaaaacgata atcaacaaca agttgggctg
     3181 gttgatgacg aaggtaatgt ttatcttgca ggcgttaagc cgggtgaaca tatgaccgtt
     3241 ttctgggagg gagaatctca ctgcgatatc agcctgccag atccgttacc gaatgacctg
     3301 ttcaatggct tgttgttgcc atgccagcaa aaagggggaa gtgctcccgt cataacgcat
     3361 gatattcaac ctgtaattca ggagcagaca caacaggtga caccaatgga accgccgatg
     3421 tctgtttcat cgaaccaata acgtgattaa ggaatgatcc atgactaaac atctctctgt
     3481 caaaacattg gcaacggtac tgtcgttact ggctgcaacg gtaagttttt ctgctctggc
     3541 gtcagtaacg cccgatcgta ctcgcctgat ttttaatgaa agcgataaat ccatcagcgt
     3601 cacgctgcgt aataacgacc cgaaacagcc ttgtctggca caaagctgga tcgaagatga
     3661 aaagggcgtc aagattagtt cgccgctggc agtattgccc cccgtacagc gcattgatgc
     3721 aatgatgaat ggtcaggtga aaattcaggg attgccagat atccataaat tgcctgccga
     3781 tcgtgaatcg atattttatt tcaatgtgcg tgaaattccg ccgaaatcga acaaagccaa
     3841 tacgttacaa attgcgctgc agacacgcat caaacttttc tggcgaccta aagccctgga
     3901 aaaagctagc atgcaaaacc catggcagca taaagtgacc ttaacccgta acggtcagga
     3961 atttaccgtc aataacccta ctccgtatta cgtcattttc agtaatgcca gcgcgcaaaa
     4021 aaatggcaat ccggcagacg gtttctcgcc gatagtgatg tcgccaaaaa cctccgcacc
     4081 gctcaaggtg aagatggcta gcgtgccggt gctgacctat gtcaacgact atggcgcacg
     4141 gatgccactc tttttcacct gtagcggcaa cagttgtcag gttgatgaag agctgagccg
     4201 caaaggttaa tgacggggaa gtaagtatga aaagatcttg ttcagtctgg gcgggaacat
     4261 ggttactgat gtgttgtatt ggtagcgact ttgcagttgc agggagtaaa tcagtaaatt
     4321 tgagtttacg agtgctcgtt gatgcccctc ctccttgcac ggttaatggt gcgacggttg
     4381 agtttggtaa tgtattcatc aataagatcg atggggtagg ttataaacaa cccattgatt
     4441 actcgcttgt ttgtaacaac ctggcaatgg atgacctgcg tttacagatg cgggcaacaa
     4501 cggttgtgat caacggtgaa acagttatca gcacaggtat ttcagggttt ggtattcggg
     4561 ttcagaaaag tagtgatcac tcaacactcg acttaacgtc tggttcatgg ttgccgttta
     4621 atttttcctc gggcgtccct gcactcgaag cagtgccggt gaaacagagt ggtacaacct
     4681 tatcggcagc ggaatttaac gccagtgcca caattgtggt ggattaccaa tgaaacggat
     4741 ctcacttctt atgctgtggg gctttagctc gatggcactc tcaaatgtct cttttcacgg
     4801 ctatttagtg cagccaccta actgcactat tagcaacggt aaaaccattg aaataacctt
     4861 tcaggatgtt tttgttgatg acattaatgg cagtaactat gagcaaaccg tgccgtattc
     4921 cattacctgt gatacggcgg ttcgcgaccc atcaatggaa atgaaattga gctggagcgg
     4981 tacgcagagc gactttgacg atgccgcggt tagcaccgat atctctggcc tggcgattcg
     5041 gcttaaacag gcagggcaga gctttaagct caatacggca ctggtggtga atgaaacggc
     5101 cttaccggtg ttaaccgcag taccggtcaa aaagagcggt gtcgatttgc ctgaggcaga
     5161 ctttgaagcc tgggccacgt tacaggtgga ttatcaatga agaaaaagcg tgcgttggtt
     5221 tttatctgta gcctgatgct gttgtacagc gttactgccc cggcggtcga caacctgcac
     5281 tttaccggta atttgctcgg taaatcctgt acgccagtga tcaacggtag cctgttggcg
     5341 gaggtgcact ttcccaccat tgccgccagc gatttaatga acctcgggca gtcagagcga
     5401 gtaccgttgg tatttcaatt gaaggattgc aaaagctcca cgctgtttag cgtcagggta
     5461 acgctgacgg gaacggaaga tagcgaactg cccggatttc tggcctttga cgcctcatct
     5521 tctgcatccg gagctgggat tggtattgaa acggcggcag gcgcggcggt gccagtcaac
     5581 agcacaacgg gtgtcacgtt cccgctaaac cagggcaata acagtctcaa ttttaatgcc
     5641 tggctacagg cgaagggcgg gcgggatgtg acgtccggtg atttcagcgc tacggcgacg
     5701 gcaacctttg agtattttta acatgaaaaa atggacaatg atttttgcga gcctggtgtt
     5761 gctggtgatg agtgcgagcg ttccaaagag ctatgccggg gataaactca ccagcgcgag
     5821 tttcgacagt gtgaagattt attatgggaa ggggaaaatg tctattcata acaccggagg
     5881 tttgtatgtc acggtgttaa ctcccaacga ggcggcctat ggggtgtaca acgatgccat
     5941 aaggaagaac gagcaagcca gcgttatttc gtggacgggg tcgggaccag cgccagtgtt
     6001 gatcttaact gactacggta atgctgatag ctcgaattgt cctgggataa atacggcgac
     6061 atttcagtgt gcatatgaga cgtttaagat aaccgttcag tccgacagtt atggttgccc
     6121 gtggctcgct tctttttata cgatcacaga tcaacccgga ttcgggagat atactgggcc
     6181 aacgaaccat gccacagtat gtccgacgat ccctgtcgcg tcgtatgaca tctcgtggag
     6241 tgagaattac gtcagccaca ataaggcgtt acagttgcaa tccaatggct cgacaatcac
     6301 caccacgctt tcgacctacc tgatggaaga tggcaaactc tgtgatggca gcgtttttga
     6361 tagcagaggt gcatactgcc gcgtggtctc ggaactgtta acctttacct cctacggttg
     6421 cgataaatca acggtaacgg tttctcccac acggcatccg gtaaccgata aacagctaca
     6481 tgatatcgtg gtaaatgtga acaccagtag ccgacaaccg attgattcta cgtgccgatt
     6541 ccagtatgta ctgaatgaat tgtagtgacg atattttcac aagtcgacgg ggccatctgg
     6601 ccctgtcgtc atttccggcg tctgttttat gatatgatgg ctaaggcgaa ctttccctct
     6661 acagcattcg atgccagcat tcagggcaat tcaggcggca gccactcttc cacttcaatc
     6721 aacaccaaca aagccgcatc gccaccatac tcttttggtg cctggtgaaa tgccattaca
     6781 tgtggatgct gcgccagcca cagcggcgtt tgctgcttca aaatatgctt cccgtgacca
     6841 tgcatcacgc aggcacaaaa cacatgttca cggcggcagg cggcaatcaa cgcccccagt
     6901 tcctgtttgg cttgcaactg cgtcagaccg tgcaaatcaa gaaacaactc cggtgaataa
     6961 tcaccgcgcc gcagtttctt cgcttcaaaa tggctgacat ccgggcgaac atatttcacc
     7021 ggaccttcgg tatttaataa tggctgaaac tcatcggaaa aataatggct ggcatcggcc
     7081 tgctcctgaa tcaaccgctt gaccggtact tcgctgattt ttttacgctg cggtcggtgg
     7141 acaatcgtgt cctgcttaat cttgcgagtc cccgccatca actggcgaaa cagcgcctgg
     7201 tcctcctcgc taagtgttgt tttctttttc atttaactgt ctctatttct aattttccta
     7261 cagtttacct gactcgtagc acaatcgatc gttttaaacg tatttttcct cactcaccgc
     7321 gcgtgaatgc tgatttatcg tcatcttcat ggcaaactag ccgccgaaat tattgcgatc
     7381 atgccctgga ggaatacgtg gataaaattt tcgttgatga agcagtaaat gagctgcaaa
     7441 ccattcagga catgttgcgc tggtcggtga gccgcttcag cgcggcgaat atctggtacg
     7501 gccacggcac cgataacccg tgggatgaag ccgtgcaact ggtgttgcct tcactctacc
     7561 tgccgctgga tattccggaa gatatgcgca ccgcgcgtct gacttccagc gaaaaacacc
     7621 gtattgttga gcgcgtgatc cgccgcgtca acgaacgcat tccggtggct tacctgacca
     7681 ataaagcctg gttctgtggc catgaatttt acgtcgatga acgcgtgctg gtgccgcgct
     7741 cgccgattgg tgaactgatc aacaataaat ttgccggact catcagcaag caaccgcagc
     7801 acattttaga tatgtgtacc ggtagcggct gcatcgccat tgcctgtgct tatgccttcc
     7861 cggaagcgga agtcgacgcg gtagacatct ctcccgacgc gctggcggtt gctgaacaga
     7921 acatcgaaga acacggtttg atccacaacg tcattccgat tcgttccgac ttgttccgtg
     7981 atttgccgaa agtgcagtac gacctgattg tcaccaaccc gccgtatgtc gatgcggaag
     8041 atatgtccga cctgccaaac gaataccgcc acgagccgga actcggtctg gcatctggca
     8101 ctgacggcct gaaactgacg cgtcgcattc ttggtaacgc ggcagattac ctcgctgatg
     8161 atggcgtgct gatttgtgaa gtgggcaaca gcatggtaca tcttatggaa caatatccgg
     8221 atgttccgtt cacctggctg gagtttgata acggcggcga tggtgtgttt atgctcacta
     8281 aagagcagct tattgccgca cgagaacatt tcgcgattta taaagattaa gtaaacacgc
     8341 aaacacaaca ataacggagc cgtgatggct ggaaacacaa ttggacaact ctttcgcgta
     8401 accaccttcg gcgaatcgca cgggctggcg ctcggctgca tcgtcgatgg tgttccgcca
     8461 ggcattccgc tgacggaagc ggacttgcaa catgaccttg atcgtcgtcg acctgggaca
     8521 tcgcgctata ccacccagcg ccgcgagccg gatcaggtca aaattctctc cggtgttttt
     8581 gaaggtgtta ctactggcac cagcatcgga ttgttgatcg aaaataccga ccagcgttct
     8641 caggattata gtgcgattaa ggacgttttc cgtccaggtc atgccgatta cacctatgaa
     8701 caaaaatacg gcctgcgcga ttatcgcggc ggtggacgtt cttccgcccg cgaaaccgct
     8761 atgcgcgttg cagcaggggc gattgccaaa aaatatctcg ccgagaaatt tggcattgag
     8821 attcgcggct gcctgaccca gatgggtgat attccgctgg aaatcaaaga ctggtcgcag
     8881 gtcgagcaaa atccgttttt ctgcccggac ccggacaaaa tcgacgcgtt agatgaactg
     8941 atgcgcgcgc tgaaaaaaga gggtgactcc atcggcgcga aagtcaccgt cgttgccagt
     9001 ggcgtacccg ccggacttgg cgagccggtt tttgaccgcc tggatgccga catcgcccat
     9061 gcgctaatga gcattaacgc ggtgaaaggt gtggaaattg gtgatggttt tgacgtggta
     9121 gcgctgcgtg gcagccagaa ccgtgacgaa atcaccaaag acggtttcca gagcaaccat
     9181 gcgggcggca ttcttggtgg tatcagcagc gggcagcaaa tcattgccca tatggcgctg
     9241 aaaccgacct caagcattac tgtgccgggg cgcacgatta accgctttgg cgaagaagtt
     9301 gagatgatca ccaaaggccg tcacgatccg tgtgttggga tccgcgcggt gccgatcgct
     9361 gaagcgatgc tggcgatcgt tttaatggat cacctgttac ggcaacgggc gcaaaatgct
     9421 gatgtgaaga ctgatattcc acgctggtaa aaatgaacaa aactgcgatt gtgctgctgg
     9481 ccctgcttgc cagtagcgcc agcctggcag cgacgccgtg gcaaaaaata acccaacctg
     9541 tgccgggtag tgcacaatcg ataggcagtt tttcaaatgg ctgtatcgtc ggtgctgaca
     9601 cgctgccgat acagtccgaa cattatcagg tcatgcgtac cgatcagcgt cgctatttcg
     9661 gacacccgga tctggtgatg tttatccagc gtctgagtcg ccaggtgagc aatctgggta
     9721 tgggtacggt gctgattggc gatatgggga tgcctgctgg tggtcgtttc aacggcggtc
     9781 acgccagcca ccagactgga ctggatgtcg atatcttcct gcaactgcca aaaacacgct
     9841 ggacctccgc gcagctcttg cgcccgcaag cgttagattt agtttcccgc gacggtaaac
     9901 acgttgtccc gacgctgtgg aagccggaaa ttttcagctt gattaaactc gccgcgcagg
     9961 acaaagacgt cacgcgcatt tttgttaatc cggcgattaa acaacaactt tgccttgatg
    10021 cgggcaccga tcgcgactgg ttacgcaaag tgcggccctg gttccagcat cgcgcccata
    10081 tgcatgtacg actacgttgt cctgccgata gcctggagtg tgaagatcaa cctttaccgc
    10141 caccgggcga tggttgcggg gcagaactgc aaagctggtt tgaaccacca aaaccgggaa
    10201 caacaaagcc tgagaagaag acaccgcctc cgttgccgcc ttcctgccag gcgctactgg
    10261 atgagcacgt gatctaatgg aaacgtttaa tagcctgttt atggtttccc cgctgctgct
    10321 gggagttctc ttttttgtcg ccatgctggc gggatttatc gactcgattg ccggtggcgg
    10381 tgggttactc accattccgg cattgatggc ggcggggatg tctccggcta acgcgctggc
    10441 aaccaataaa ctgcaagcct gcggcggctc tatttccgcc actatctact ttattcgccg
    10501 caaagtggtt agtttaagcg atcagaaact caatatcgcc atgacctttg tcggctcaat
    10561 gagcggcgca ttactggtgc agtatgttca ggctgatgtc ttacggcaga ttctgcccat
    10621 tctggtgatt tgtatcggcc tctatttctt gctgatgcca aagctgggtg aagaagatcg
    10681 ccagcgccgg atgtatggac tgccatttgc gctggtggcg gggggctgcg tcggttttta
    10741 cgatggattc tttgggccag ctgccggatc gttttacgcg ctggccttcg ttacgctgtg
    10801 cggattcaac ctcgccaaag ccacggctca cgccaaatta ctcaacgcaa cgtcaaacat
    10861 tggcggtctg ctgctgttta ttctcggcgg caaagtgatt tgggcgacgg gctttgtgat
    10921 gctggtgggg cagttcctcg gcgcgcgcat gggttcaaga ctggtgttga gcaaaggaca
    10981 aaagctgatc cgcccgatga tcgttatcgt ctcggcggtg atgagtgcaa aattacttta
    11041 cgatagccac ggacaggaga tcctccactg gttggggatg aactaatgaa cagtacacac
    11101 cactatgagc agttgattga gatttttaat agctgctttg ccgatgaatt taatacccgt
    11161 ctgattaaag gcgacgacga accgatctat cttcctgctg atgcggaagt accgtataac
    11221 cgaatcgtct ttgctcatgg cttttatgcc agcgcaattc atgagatttc gcactggtgt
    11281 attgccggaa aagcgcgccg tgagcaggtt gatttcggct actggtattg cccggacggt
    11341 cgcgatgccc agacgcaaag ccagtttgaa gatgttgaag tgaagccgca ggcgctggac
    11401 tggttgttct gcgtggcggc gggatatccg tttaatgtta gctgcgacaa tctggaaggg
    11461 gatttcgaac cggaccgcgt ggtgtttcaa cggcgggtac atgcgcaggt aatggattat
    11521 ctggcaaacg gcattcccga acgtccggca cgcttcatta aagcactaca aaattattat
    11581 cacacgccgg aacttacggc ggaacagttc ccgtggccgg aagcgctcaa ctgaagccag
    11641 cgccacgttt tacaatgagg aaagaagatg atcgcggagt ttgaatcacg cattctggca
    11701 ttaatcgacg gtatggttga ccatgccagt gatgatgagt tgtttgccag tgggtatttg
    11761 cgtggccacc tgacattagc catcgcagaa ctggaaagtg gtgatgacca ctccgcccag
    11821 gcggtacata caaccgttag ccagagtctg gaaaaagcga ttggtgccgg tgagttgtcg
    11881 ccgcgtgacc aggcgctggt gaccgatatg tgggaaaacc tgtttcagaa ggcgtcacag
    11941 cagtaagtaa tgtgccggat aaggcgttca cgccatatcc ggcaagcaat gccagagctg
    12001 attaccccgc cttaaccgct ttacctttca acaacttccg cacccataac cgattcgggt
    12061 ttaacgccgc cagcgtactg gcatccatcg gaattggttc gttgctcatc tgcgccgcca
    12121 gaatctcggc acacaacggg gcagagcaca aaccgcgaga acctaaagca gctaacataa
    12181 aaagatcgtc ataaattggc gcgcttactg cctcatcttt ctgctccgcc agcgaagcat
    12241 attccacgag cgttacgtca taatcgggca cgttgcctac cattggcaga tgatcgcggg
    12301 tggcacaacg cacaccgcag cgcgcctctt tctcactgac atcaacctct tttgcccact
    12361 gcgcctgcgg gaaacaatca atcaaccgct ggcgattctg ctgctgatcg tcctcactgt
    12421 acgccgtatc ttcgctgccg cgatgataac tggcaccaat acaatgatgt tgattcgccg
    12481 gattttgtgg cgtgagataa ccgtcatagc acagcacctg cttcagctcc gctagttccg
    12541 gcgttgtcgg aatatggcta acctgcccgg caaccgaata cactggcaga gacgatgttt
    12601 ggctgaaacg gctgatttga tgcccgttcg ccagtaccac cacgctatgc gttgcttgct
    12661 gatctcctgc aaaattcagc aaccaacagt catccttacg ggataaatcc tttaactggt
    12721 gttgataacg aatctgtaaa ccttgctgtt gcgccagttt cagcacatta caggtcagtt
    12781 ctgctggaca cagccatcca ccttgcggat atgtaatacc gccacaattt gttactacac
    12841 ctgtaacgcc ttcaacctca ttcgccccga ccgcaacagc cagttctgtg ggtaaatcca
    12901 tcgacagcat ctgtgcgatt ttatgctggc ttttctcatc ccagcctaac tgcgtgacgc
    12961 cgcaccagtc atgatcaaat ttgacgggca ataaatcgta aagccgacga gcaaaagtaa
    13021 acgcattaga gaaaaagcgg ttcaacgcct catcgtgttt acttaagagc ggatacagcg
    13081 ccccctggcg attgccggaa gcacccagcg cgggggcttc atccgcgcaa taaagcgtta
    13141 cttgccagcc gcgccgtaat aacgccagcg acaacaacgc gctggcaata ccaccgccga
    13201 taatcgctac ttcccgtttg ctgctgcccg tacgattaaa ccacggcgtg gagcagggga
    13261 ggggtaatgt ctgttccatc accccacaaa gcatttcccg tttgcgccca aagcccttac
    13321 gtttttgcat cgtgaatccg gcgtcctgca aaccgcggcg gacaaaaccg gcagacgtaa
    13381 atgtcgccag cgtgctgccc ggacgcgcca gtcgtgccat ggcgttaaac agattttgcg
    13441 tccacatatc cgggtttttc gctggcgcaa agccgtccag aaaccaggcg tccacttttt
    13501 gattcagcga gtcgtccagt tggctggtca gttcgtttat atcgccaaac cataaatcca
    13561 gtgtcacgtg gccttcatcg agcaataaac gatggcaacc gggcaagggc attggccact
    13621 gcgcctgaag ttgttctgcc cacggagcca gttccggcca gtgtagatgc gctaaggcta
    13681 aatccgcacg ggtgagggga aatttctcaa aactaatgaa atgtaagcgt tgtaattgcg
    13741 cttgcggatg tgcttcgcga aactgatcaa atgcctgcca tagtgtcagg aagtttaatc
    13801 cggtgccgaa gccgctctct gctaccacaa acagaggatg tggatgctca ggaaagcgtg
    13861 cctctaattg gttacctccg agaaaaacat aacgcgtctc ttccagtccg ttgtcattgg
    13921 aaaagtaaac gtcgtcaaaa tctcgggaaa caggtgtacc ctcagcatta aattcgaggt
    13981 tggcaggttg tatggcgtag tgtttcacgt aagttactcg tctgacgggc agtctgacga
    14041 tcttaacgat gtgtgtcagg ctgcgcaaat ttctctatta attggctgat cggacttgtt
    14101 cggcgtacaa gtgtacgcta ttgtgcattc gaaacttact ctatgtgcga cttacagagg
    14161 tattgaatga aacgtgcagt gattactggc ctgggcattg tttccagcat cggtaataac
    14221 cagcaggaag tcctggcatc tctgcgtgaa ggacgttcag ggatcacttt ctctcaggag
    14281 ctgaaggatt ccggcatgcg tagccacgtc tggggcaacg taaaactgga taccactggc
    14341 ctcattgacc gcaaagttgt gcgctttatg agcgacgcat ccatttatgc attcctttct
    14401 atggagcagg caattgcaga tgctggcctc tctccggaag cttaccagaa taacccgcgc
    14461 gttggcctga ttgcaggttc cggcggcggt tccccgcgtt ttcaggtgtt cggcgctgac
    14521 gcaatgcgcg gcccgcgcgg cctgaaagcg gttggcccgt atgtggtcac caaagcgatg
    14581 gcatccggcg tttctgcctg tctcgccacg ccgtttaaaa ttcacggtgt aaactactcc
    14641 atcagctccg cgtgtgcgac ctccgcacac tgtatcggta acgcagtaga gcagatccaa
    14701 ctgggcaaac aggacatcgt gtttgctggt ggcggcgaag agctgtgctg ggaaatggct
    14761 tgtgaattcg acgcgatggg tgcgctgtct accaaataca acgacacccc ggaaaaagcc
    14821 tcccgtactt atgacgctca ccgtgacggt ttcgttatcg ctggcggcgg cggtatggtg
    14881 gtggtcgaag aactggaaca cgcgttggca cgtggtgctc atatctacgc tgaaatcgtt
    14941 ggctacggcg caacgtctga tggcgcagac atggttgctc cgtctggcga aggcgcagtg
    15001 cgttgcatga agatggcgat gcacggtgtt gataccccaa tcgactacct gaactcccac
    15061 ggtacttcga ctccggttgg cgacgtgaaa gagctggctg ctatccgtga agtgttcggc
    15121 gataagagcc cggcgatttc tgcaaccaaa gcaatgaccg gtcactctct gggcgctgct
    15181 ggcgtacagg aagctatcta ctctctgctg atgctggaac acggctttat cgccccgagc
    15241 atcaacattg aagagctgga cgagcaggct gcgggtctga acatcgtgac cgaaacgacc
    15301 gatcgcgaac tgaccaccgt tatgtctaac agcttcggct tcggcggcac caacgccacg
    15361 ctggtaatgc gcaagctgaa agattaattc ggagtaggtc ggataggacg cgtcagcgtc
    15421 gcatccgacg atatgcggca atgctgcctc cggtactaaa gaataaggga accctatggt
    15481 tccctttttc acatcattga caatcgccgc ctgtaccagg caaacttccc gctttgtcga
    15541 tttccttctg aaaagacgta cgcgttaatt cctgccaacg caccgtaacc ctgaaaccag
    15601 agagatgaga cggggatact cctcgccttg cgctgcattc tggagtaatg catgactgct
    15661 gtaagccaaa ccgaaacacg atcttctgcc aatttttcgc tcttccgcat cgcttttgcg
    15721 gtttttctca cctacatgac cgtagggttg ccgttgccgg ttatcccgct gtttgttcat
    15781 catgaactgg gctatggcaa cactatggtc ggcattgccg tcgggattca gtttctggct
    15841 acggtgctga cgcgtggtta cgccgggcga ctggccgatc aatatggcgc aaaacgttcg
    15901 gcgcttcagg ggatgttagc ttgtggtctg gcggggggcg cgttgctgct ggcggcgatt
    15961 ttgcccgtcc ccgcaccgtt caaatttgcc ctgttggtcg tcgggcgttt gattcttggc
    16021 tttggtgaaa gccagttact gacgggcgct ctgacctggg gattaggcat cgttgggcca
    16081 aaacattccg gcaaagtgat gtcatggaac ggaatggcga tttacggtgc cctcgctgtt
    16141 ggtgcgccgc ttggcctatt gattcatagc cattacggtt ttgccgcact ggcgctcacc
    16201 acaatggcat tacccttact ggcgtgggcc tgtaacggca cagtgcgcaa agtaccggcc
    16261 ctggcggggg aacgtccatc gctgtggagc gttgtcgggc ttatctggaa accagggtta
    16321 ggtctggcac tacaaggcgt tggttttgcg gttatcggga ctttcgtttc gctctacttt
    16381 gccagcaaag gatgggcgat ggcgggcttt acgcttaccg cgtttggcgg cgcatttgtc
    16441 gtgatgcgcg tcatgtttgg ctggatgccg gaccgttttg gcggcgtgaa agtggcgatc
    16501 gtctctctgc ttgtagaaac ggtgggcttg ttgctgctct ggcaagcccc aggtgcatgg
    16561 gtcgcattag cgggcgcggc gttaaccgga gccggatgtt cgcttatctt tcctgcgctg
    16621 ggcgtggagg tggttaaacg cgtaccgtca cacgttcgcg gcaccgcact gggcggttac
    16681 gccgcgtttc aggatatcgc cctcggcgtc tccgggccgc ttgcgggaat gctggcgacc
    16741 acgtttggtt actcttcggt atttcttgcc ggggcgatct ctgcggtgct gggaattatt
    16801 gtcacgatac tgtcatttcg tcggggttaa cgaaccagcc agaccagcat caggataatc
    16861 gcgactaaca gtatccacag cgcgggacgc gttgccatat tttgcaacgt gtccatcatc
    16921 ggtggaaaag gattaagcag cggttgcata tcacgcggat caatcccttt aacccgccgt
    16981 tgataaagct gattaagaat atcctcggcc tgggcagaag aaagcgatga ttgcgcagaa
    17041 aggccatatt tttgctggat atatgccgat aacgccgcca gttcactggc atctaaaggt
    17101 tgtttcagcg ccatctgtag tgattcaagc gtcggcgtat tttgctggct tagcgtctga
    17161 cgcgcctgca gccaggtcac cagatggtta aacagtttcg ctggaattaa ctcgccatct
    17221 ttcaccccgg aaagttccag catcgattgc cagatctgtt tgctgggttc tcccgttgcc
    17281 gccgcaagtt tggtcaccag ctgtttgagc gcattgtgct ccgccggtaa taacggacgg
    17341 tcggtcgcct cgcgctgctg cggttgcgga ataaccatct tcccttcctg caacagggtt
    17401 agaatggttt ttaattgctc cggtgagagc tgattcagcg gcgtctgacc aaagttatga
    17461 cggatataat ccgtgaccgc ctgacgatta ttccccagac gtaaatactc ccctaattgc
    17521 gctaaaagct ggcgggcaga atggcttttt tgcgcggcca gcagacgttg cgccagattg
    17581 tgctcagcgg cagggaagtg acgcgaaagc agcggtgaat ctcccgacag accaatatcg
    17641 tgcctgatac ccgcccacag ttctgctctt tgctgttgcg tcagtgaggt cactttcgtc
    17701 attaagcttt ccagcgaagt acgttgctga ctggataaag gctgattgcc cgcgccagac
    17761 ggcaggttat ctccctgacc tggtggttgc ccaggaggag ggccggaaat aggttgtatc
    17821 attacgtatc cttatacctg aaatcttcgc aagtatgcct ggccgcgaga ttatggcaca
    17881 cttgtccggt taactctcgt ctcatacagg taacacaaac gtgaaaatcc ttgttgatga
    17941 aaaatgaaaa ttatttaaca ttttgatata aaaggattta tatcaattgt tgtccacgca
    18001 atgaccacat tttcgagata tagaaaaagc ccgcagtttt tacgctgcgg g
//
LOCUS       DAZFLD010000095        18046 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.095, whole genome shotgun sequence.
ACCESSION   DAZFLD010000095 DAZFLD010000000
VERSION     DAZFLD010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18046)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18046)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18046
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.095"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(57..1085)
                     /gene="epmB"
                     /locus_tag="ACHKBL_002708"
     CDS             complement(57..1085)
                     /gene="epmB"
                     /locus_tag="ACHKBL_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0072581 - protein-N6-(L-lysyl)-L-lysine
                     modification to protein-N6-(beta-lysyl)-L-lysine [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HFO5819561.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPNDPLLRQVLTSQDEFVVAPGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITDALVERFSHSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
     gene            1127..1693
                     /gene="efp"
                     /locus_tag="ACHKBL_002709"
     CDS             1127..1693
                     /gene="efp"
                     /locus_tag="ACHKBL_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFO5819562.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            1745..1870
                     /gene="ecnA"
                     /locus_tag="ACHKBL_002710"
     CDS             1745..1870
                     /gene="ecnA"
                     /locus_tag="ACHKBL_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HFO5819563.1"
                     /translation="MMKRLIVLVLLASALLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            1981..2127
                     /gene="ecnB"
                     /locus_tag="ACHKBL_002711"
     CDS             1981..2127
                     /gene="ecnB"
                     /locus_tag="ACHKBL_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HFO5819564.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            2302..2619
                     /gene="sugE"
                     /locus_tag="ACHKBL_002712"
     CDS             2302..2619
                     /gene="sugE"
                     /locus_tag="ACHKBL_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HFO5819565.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(2620..3153)
                     /gene="blc"
                     /locus_tag="ACHKBL_002713"
     CDS             complement(2620..3153)
                     /gene="blc"
                     /locus_tag="ACHKBL_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HFO5819566.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDREMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(3242..4375)
                     /locus_tag="ACHKBL_002714"
     CDS             complement(3242..4375)
                     /locus_tag="ACHKBL_002714"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HFO5819567.1"
                     /translation="MFKMTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGI
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDDVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANASIGLFGALAVKPSGLSFEQAMQTRVFQPLKLTHTWINVPSAEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLKPLDITEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDIIINGSDNKIALAARPVKAITPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAACQILNALQ"
     gene            complement(4438..4797)
                     /gene="frdD"
                     /locus_tag="ACHKBL_002715"
     CDS             complement(4438..4797)
                     /gene="frdD"
                     /locus_tag="ACHKBL_002715"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HFO5819568.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            complement(4808..5203)
                     /gene="frdC"
                     /locus_tag="ACHKBL_002716"
     CDS             complement(4808..5203)
                     /gene="frdC"
                     /locus_tag="ACHKBL_002716"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HFO5819569.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            complement(5214..5948)
                     /gene="frdB"
                     /locus_tag="ACHKBL_002717"
     CDS             complement(5214..5948)
                     /gene="frdB"
                     /locus_tag="ACHKBL_002717"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HFO5819570.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            complement(5941..7749)
                     /gene="frdA"
                     /locus_tag="ACHKBL_002718"
     CDS             complement(5941..7749)
                     /gene="frdA"
                     /locus_tag="ACHKBL_002718"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HFO5819571.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAVIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            8074..9051
                     /gene="epmA"
                     /locus_tag="ACHKBL_002719"
     CDS             8074..9051
                     /gene="epmA"
                     /locus_tag="ACHKBL_002719"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HFO5819572.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            9270..10772
                     /gene="yjeM"
                     /locus_tag="ACHKBL_002720"
     CDS             9270..10772
                     /gene="yjeM"
                     /locus_tag="ACHKBL_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
                     /protein_id="HFO5819573.1"
                     /translation="MPHTIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAIPFYIF
                     SALLFFIPFALMMAEMGAAYRKEEGGIYSWMNNSVGPRFAFIGTFMWFSSYIIWMVST
                     SAKVWVPFSTFLYGSDMTQHWRIAGLEPTQVVGLLAVAWMILVTVVASKGINKIARIT
                     AVGGIAVMCLNLVLLLVSITILLLNGGHFAQDINFLASPNPGYQSGLAMLSFVVFAIF
                     AYGGIEAVGGLVDKTENPEKNFAKGIVFAAIVISIGYSLAIFLWGVSTNWQQVLSNGS
                     VNLGNITYVLMKSLGVTLGNALHLSPEASLSLGVWFARITGLSMFLAYTGAFFTLCYS
                     PLKAIIQGTPKALWPEPMTRLNAMGMPSIAMWMQCGLVTVFILLVSFGGGTASAFFNK
                     LTLMANVSMTLPYLFLALAFPFFKARQDLDRPFVIFKTHMSAMIATVVVVLVVTFANV
                     FTIIQPVVEAGDWDSTLWMIGGPVFFSLLAMAIYQNYCSRMANKPELALD"
     gene            10835..11149
                     /gene="yjeN"
                     /locus_tag="ACHKBL_002721"
     CDS             10835..11149
                     /gene="yjeN"
                     /locus_tag="ACHKBL_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000340721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjeN"
                     /protein_id="HFO5819574.1"
                     /translation="MDDASRDPVITEDEIRELQFSAGDVAEIEQTVLSFVDTRHTRKV
                     AMVVGNTINTLKERDGPRWGNLPDIYCAYLIRCLVFRGELVGYGDLFRMRYSEIKRPI
                     IS"
     gene            11146..11460
                     /locus_tag="ACHKBL_002722"
     CDS             11146..11460
                     /locus_tag="ACHKBL_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeO family protein"
                     /protein_id="HFO5819575.1"
                     /translation="MSARMFILCCIWFIVAFLWITITSALDKEWMIDGRGINNVCDVL
                     MYLEDDDTRDVGVIMTLPLFFPFLWFALWRKKRGWFMYATALAIFGYWLWQFFLRYQF
                     CL"
     gene            complement(11491..14814)
                     /gene="mscM"
                     /locus_tag="ACHKBL_002723"
     CDS             complement(11491..14814)
                     /gene="mscM"
                     /locus_tag="ACHKBL_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HFO5819576.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQISQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQEVIDNYPKLSATLRAQLNNMRDEPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNSPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMDLVASQQRQAASQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNGQLEDALKEVNEATHRYLFWTSDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLLGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIVPLIMALMMFDNLDDRE
                     FSGSLGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVA
                     ILASAVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAE
                     MLAQRARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLW
                     SEIHSAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLE
                     LAILQHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFG
                     LQEIFANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNK
                     AFITEQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFL
                     VDLQQGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNG
                     KQTGRTLTSAGKGRQAGSL"
     gene            complement(14836..15804)
                     /gene="asd"
                     /locus_tag="ACHKBL_002724"
     CDS             complement(14836..15804)
                     /gene="asd"
                     /locus_tag="ACHKBL_002724"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaetidylserine decarboxylase"
                     /protein_id="HFO5819577.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLPAEEIEAEHDANPLVDDNKDQV"
     gene            complement(15901..16953)
                     /gene="rsgA"
                     /locus_tag="ACHKBL_002725"
     CDS             complement(15901..16953)
                     /gene="rsgA"
                     /locus_tag="ACHKBL_002725"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HFO5819578.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            17048..17593
                     /gene="orn"
                     /locus_tag="ACHKBL_002726"
     CDS             17048..17593
                     /gene="orn"
                     /locus_tag="ACHKBL_002726"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HFO5819579.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEAYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
     gene            17804..17879
                     /locus_tag="ACHKBL_002727"
     tRNA            17804..17879
                     /locus_tag="ACHKBL_002727"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:17837..17839,aa:Gly,seq:gcc)
BASE COUNT         4161 a         4626 c         4760 g         4499 t
ORIGIN      
        1 tatgaataat taaaggttat gcaacgtgca aagattaaac ttccctgttt aatcagttac
       61 tgctggcgta gctggagatc cagcggcgtt ttgctgggtt cgccgccaat ttctcgcgcc
      121 agtttaggca ccagatatcc cgacaccagt gtcagcaact cacgcataat ctgccgtgct
      181 tcgtcatcac tcaccataaa atgcgccgcg ccctgtactt tatcgagcac atgcaggtaa
      241 tacggcatca cgcccgcatc gaacaacgca ttactcaggt ttgccagcgt ttgcgcgtta
      301 tcgttcacac cacgtaacag aacgctctgg ttgagcaaag taacgcctac ccggcgcaac
      361 ttagccatcg cctgacggaa tgtttcatct acctcattgg catggttaat gtgattcacc
      421 agcaagattt gcagcgtaga atgagaaaag cgttcaacca gcgcatctgt gatgcgtgcc
      481 gggatcacaa tcggcagacg gctgtgaatc cgcagacgtt tgatatgcgg gatggcttcc
      541 agttgtgtga gcaaccagtc cagctcgtga tctttcgcca tcagcggatc gccgccggag
      601 aaaatcatct cgtccagttc cggatgcgca gcaacatact caagtgcagt ctgccagtta
      661 cgcttgttgc cctgattttc ggcatagggg aagtggcgac ggaagcaata gcggcaattt
      721 accgcgcagc cgcctttgac cagcaaaagc gcccggttgt ggtatttatg caacaaacct
      781 ggcactacgc tgtgctgttc ttccagcggg tcggtggaga atccgggcgc gacaacaaac
      841 tcatcttgcg aggtaagtac ctgacgcaaa agcggatcgt tcggattgcc tttctccatg
      901 cgatcgataa atgagcgggg cacacgcagg gcaaacagct ttttggcgct gcgcccggcc
      961 aacagttttt cgtccgcgtc tatattcaaa agacgcagaa gttcatcagg atcggtcaca
     1021 acatcggcaa gttgcgttaa ccaatcttct ctggatgggg tatttagggt tacaatatgc
     1081 gccattttgt ggcttagcta ccaattaaca aatttcagag ggccttatgg caacgtacta
     1141 tagcaacgat tttcgtgctg gtcttaaaat catgttagac ggcgaacctt acgcggttga
     1201 agcgagtgaa ttcgtaaaac cgggtaaagg ccaggcattt gctcgcgtta aactgcgtcg
     1261 tctgctgacc ggtactcgcg tagaaaaaac cttcaaatct actgattccg ctgaaggtgc
     1321 tgatgttgtc gatatgaacc tgacttacct gtacaacgac ggtgagttct ggcacttcat
     1381 gaacaacgaa actttcgagc agctgtctgc tgatgcaaaa gcaattggcg acaacgccaa
     1441 atggttgctg gatcaggcag agtgtatcgt aactctgtgg aatggtcagc cgatctccgt
     1501 tactccgccg aacttcgttg agctggaaat cgttgatacc gatccgggcc tgaaaggtga
     1561 taccgcaggt actggcggca aaccagctac cctgtctact ggcgctgtgg ttaaagttcc
     1621 gctgttcgta cagatcggcg aagtcatcaa agtggatacc cgctctggtg aatacgtctc
     1681 tcgcgtgaag taatgcggtt gtggtgcggc ctgcaggctg caccatcact tattcaggtc
     1741 agagatgatg aaacgcctta tcgttcttgt tttgcttgcc agtgcgctgc tcacgggctg
     1801 taacaccgct cgcggttttg gtgaagacat taaacatctc ggcaactcca tctcccgcgc
     1861 tgccagctaa tttttcttct cctccgaaaa atcatcagat ttccatcatt tttggcgatg
     1921 ttgtctatta ttaatttgct ataggcaaac ataaataaca ttacctaaaa ggaagacgtt
     1981 atggtgaaga agacaattgc agcgatcttt tctgttctgg tgctttcaac agtattaact
     2041 gcttgcaaca ccacgcgtgg cgttggtgaa gacatttctg atggcggtaa cgcgatttct
     2101 ggcgcagcaa cgaaagcgca gcagtaagca ataacggtac gacagctgtg tcgtgccgtt
     2161 tgtttttttg cgatagtcac aaaggtaata gttgaaattc ccctgccacc tggcaaaata
     2221 tccgttcaac catcagcttt gcaggacgac ctgcaaacgc ctcttttcac cggggacggc
     2281 cccaattctc cggagcctga tatgtcctgg attatcttag ttattgctgg cctgctggaa
     2341 gtggtatggg ccgttggcct gaaatatacc cacggcttta gtcgtttgac gccaagtgtt
     2401 attactgtga cggcgatgat tgtcagtatg gcgctacttg cctgggcgat gaaatcgtta
     2461 ccagtaggga cggcttatgc cgtgtggacg ggtattggcg cagtcggcgc ggccatcacc
     2521 ggcattgtgc tgctcggcga gtccgctaac ccgatgcgcc tggcgagtct ggcgttaatc
     2581 gtattgggga ttattggtct gaaactcagc actcactaac taactaccgg gctgctgtac
     2641 ccaaataaac ttactgacat caaacccttc ccgggtcgcg actgccagca tctcctgttt
     2701 cacttcgtca gaaatggttg gcgtgcggga gagtatccac aggtagtcgc ggtccggacc
     2761 gcaaaccagc gcatggcggt attcccgatc gagtgcaata acgttataac cgccatagaa
     2821 aggaccaaag aatgacactt tcagcgcagc gcgagttggt gcgccggtaa agtacgcttt
     2881 cccttcactc tgctgccaca tttctctgtc agggttatag cctttattaa tgacattcag
     2941 gccgccgtca tcacgcaggc tgtatgttgc ggtgactttt tccagtccac gttcaaaacg
     3001 gtgatcaaaa cgggcaatct cataccaggt accgagatag cgtttagcat cgaaattatt
     3061 cactacggtc acgccacggg gcggcgtagg agaactgcag gcaacgacca gaaatgcagc
     3121 tgtcgctgcg gcaacgagag ggagcaggcg cataaatgtt tccttactgg tttttttcta
     3181 agtgtagatg acagcaagga aaagcggaga aaaggtccaa aaattcggac ccgatgaaat
     3241 tttactgtag agcgttgaga atctgacagg cggcggcgac tctcgctgga ttgggatagt
     3301 ttttgttagc cagcatcacg atacccagct ctttttctgg aataaacgcg acatagctac
     3361 caaatccgcc ggtcgccccc gttttatgta cccatgatgc gcgtactgca ggagttgggg
     3421 gcgtaatcgc ttttacgggg cgtgctgcca gtgcaatttt attgtcactg ccgttaatga
     3481 tgatgtcagg attgaccggc cagtccagca tttcccagcc caggccctga tacatatcgc
     3541 cggtttgcca gtagcgagat tgtgccagtt gtatcccttg ttgaagcgtt ttctcagtga
     3601 tatcaagggg ttttaaattg ctttgcaccc agcgggccat atcttcaatg gtcgacttca
     3661 caccataagc ctcagcatct aacgcccctg gcgaaacatg cactgcctta ccttcgcgat
     3721 atccccaggc gtaattcttt tcttctgcgg acggcacatt aatccacgta tgagtgagtt
     3781 tgagtggctg gaaaacacga gtttgcatcg cctgttcaaa gctcaaaccg gatggtttca
     3841 ccgccagtgc accgaacaac ccaatactgg cgttggcata cagacgttgt gttcctggag
     3901 cccatgcagg ttgccagttt tgatagaagc gcagcaagtc gcttgaggat ttaacgtcat
     3961 ccggcacctg caatggcagg ccgccagcgg tgtaggtcgc gagatgtaat agtgtgatcc
     4021 cattccattg tttagcggta agttcaggcc agtattttgt cgcgggatcg cttaacttga
     4081 tttcccctcg agcaatagcg tcgccaccaa gcacgcccgt aaatgttttg ctgaccgaac
     4141 ctaactcaaa caacgtttgc tgtgtgacgg gctgcttttt ggcgatgtcc gcatagcccc
     4201 aggtaaagta ataaggttta ccctgataaa ttaccgccac cgctataccg gggatctttt
     4261 gttgctctat aagcggggta attgtgcgat gcacaatatc gttgatttgt tgaggggcag
     4321 caaatgtgga gcaagaggcg gtaattaata aggcgcagag cgtcattttg aacatagggt
     4381 ctggtttcca tacaaaacgg cccgccatag gcgggccgga tttacattgg cgatgcgtta
     4441 gattgtaacg acaccaatca gcgtgacaac tgtcaggata gcagccagac cgtagaaaac
     4501 ccatttgccc gcaggtacgt ggattttcag atcgtgcatc gcgtggtgca tacggtgtaa
     4561 accacaccac agcggcagaa cgatcatcag gaacaggaat acgcgaccaa tgaagctctg
     4621 cgcgaacgcc agaacgcgct cgtagctcag tgcgtcaccc ggaaacagcc ccagtggcag
     4681 cagaatacca accagcagga tcatcaccgg cgcaataatt gcgctccaca taccaccggc
     4741 cccgaagagg ccccagaata ccggttcgtc agaacgcttt ggatttggat taatcatctc
     4801 aggctcctta ccagtacagg gcaacaaaca ggattacgat ggtggcaacc acagttaccg
     4861 cccagaggct tttgataatt ggctctggtc ccattttttc gtcttttaca atgatattgg
     4921 ccgcttttgg tgccagctca aaccaggttt tggtgtgcag cagggctgcc gccagagtga
     4981 tcaggttaat gatcacgata accgggtttt gtaaaaagtc gacgaatccc gcccaggctt
     5041 ccgggccatt tttcagggca aacagcccga aaatcagttc aatgctgaac cacacagccg
     5101 gaaccgccgt gccttcgcgc agcatgtaga agcgataaaa cggcaatttt ttccaccagg
     5161 tggacgtcat tggccgtaca tacggtttac gtttagtcgt catgttgcac tccttagcgt
     5221 ggtttcaggg tcgcgataag aaagtctttc gaactttcta ctttgccctg ctgaatggcc
     5281 gcagccggat cgacgtgttt cgggcagact tcggagcagt agcccacgaa agtacagctc
     5341 catacgccgt tctggctgtt caactgcgcc atacgctcct tcttaccgtg gtcgcggcta
     5401 tcttcgttat aacgatgcgc cagcgtaatg gcagccggac cgatgaactc tgggttcagg
     5461 ccaaactgcg ggcacgcggc gtagcacaga ccacagttga tgcaaccgga gaactggtga
     5521 tacttggcca tctgcgccgg ggtctggatg ttagtaccct gatccgcggt gcgggagttg
     5581 ccgatgatgt acggtttgat cgcttccaga ctttcgatga agtgggtcat atcgaccacc
     5641 agatcgcgtt caatcgggaa gttagctaac gcttcaacct tcataccgtc ggtgtaatca
     5701 cgcaggaagg ttttacatgc cagttttggc acgttgttaa ccatcatgcc gcaggaacca
     5761 caaatcgcca tacggcagga ccagcggtag ctcaggtccg gtgccaggtt gtctttgatg
     5821 tagcccagcg catccagtaa tgaggtagtt gcgtcataag gcacttcata gaatgcgcta
     5881 tgcggtgcgg tatcgacttc cgggttatag cgcaccacct caattttcag gtttttcatc
     5941 tcagccattc gccttctcct tcttattggc tgcttccgcc ttatcggctg catccgcttc
     6001 gccaccgtaa acgcgtttag ctggcggcag cgtagtaatc ttcacgtcgc tgtactccag
     6061 gcgagtcgtg ccatcagcat cgcggaaggc gagggtgtgt ttgaggaagt tgacgtcgtc
     6121 acgctcggtg caaccttcgt ccagacgctg gtgtgcgccg cgggactctt tacgtgccat
     6181 tgcggagtgc gccatacatt cagcaacgtt cagaccgtgg cccagttcaa tggtgtagag
     6241 caggtcggtg ttgaacacgc tggaagtgtc ggtgatgcgc acgcgtttga agcgttcctg
     6301 cagctctgcc agcttgtcga tggttttctg catcagttcc ggcgtacggt agataccgca
     6361 gccttcttcc atagccaggc ccatttcgtc gcggatcttc gcccagtttt cgccgccatc
     6421 ctggttaacc agatctttca aacgttgttc aacgccagct gcctgcgctt caattaccgc
     6481 ttcgttgcca ttaccggcag ttgctgcacg ctctgtcgct tgttcaccgg ccagacggcc
     6541 gaagaccacc agttccgcca gggagttaga acccagacgg tttgcaccgt gcagaccaac
     6601 agaggaacat tcacccacgg cgaacagacc tttaatgcgg gtttcacagt tctgatcggt
     6661 ttcgataccg cccatggtgt agtgtgcggt cggacgtacc ggaatcggtt ctttaaccgg
     6721 atcgacgcca acgtacgctt tcgccagttc gcagatgaac ggcagacgtt catgcagttt
     6781 tttctcgccg aggtgacgca agtcgagata aaccacatcg ccacgcggcg tggagatagt
     6841 gttgccttta cgccattcgt gccagaaggc ctgagaaact ttgtcgcgtg gacccagttc
     6901 catatatttg tttttcggct cgcccagcgg agtttccggg cccatgccgt aatcttgcag
     6961 ataacggtag ccatttttgt tgaccagaat accgccttca ccgcggcagc cttcggtcat
     7021 caggataccg gaacctggca gaccggttgg gtgatactga acgaattcca tgtcacgcag
     7081 cggaacgccg tggcttagcg ccatacccat accgtcaccg gtaacgatgc cgccattggt
     7141 attgtaacga taaacgcgac ccgcaccgcc ggtagccata acgaccgcgt tagcacggat
     7201 ctgcaccagc gtgccttcca tcatgttcat tgctaccagg ccgcgaacat gaccatcatc
     7261 aaccagaata tccagtacga aatgttcgtc aaaacgctgg atctgcggga attgcagaga
     7321 ggtctggaac agcgtgtgca gcatatggaa gccggtctta tcggcggcga accaggtgcg
     7381 ctcgattttc atgccgccga agcgacgtac gttgacgcta ccatccgggc gacggctcca
     7441 tgggcatccc cacagttcca gttgggtcat ttcggtgggg cagtggtgga cgaaataatc
     7501 cacgacatcc tgctcacaca accagtcgcc acccgctact gtatcgtgaa agtgatattc
     7561 gaagctgtca tgatcctgcg cgacagcggc ggagccccct tctgcagcaa cggtatggct
     7621 acgcatcggg tatacttttg agattagtgc gatttttgca tttggatttg cctgcgcggc
     7681 agcaattgca gcacgtaatc ccgcgccacc ggcgcctaca atggcaagat cggcttgaaa
     7741 ggtttgcacg acattcctcc agattgtttt tatcccacag ccccgtactt cagggtaagt
     7801 gcctgaaagt tacggtctgc gaacgctatt ccactgctcc tttataggta caacagtata
     7861 gtctgatgga taagtctgaa atttgacgag atcgattttt ttagtgcgca agagggctaa
     7921 attatcactg aagatgatta atttaattac taaaccatca gatcgtgctt tttttagtca
     7981 ctgcccaccc ttgtttgatt cctgcgtaaa atttgtctcg ccgtcgcgtt gcgagtagac
     8041 ttcgtgccct tgtcaaaaac tggagattta actatgagcg aaacggcatc ctggcagccg
     8101 agcgcatcca ttcctaactt attaaaacgc gcggcgatta tggcggagat ccgtcgtttc
     8161 tttgccgatc gtggagtgct ggaggtggag acgccttgta tgagccaggc gacggtaacc
     8221 gatattcatt tggtcccgtt tgagacacgt ttcgtaggcc ccgggcattc gcaggggatg
     8281 aatctctggt taatgaccag cccggaatac catatgaaac gcctgctggt tgctggttgt
     8341 gggccggtat tccagctgtg ccgcagcttc cgtaatgaag agatggggcg ttatcacaac
     8401 cctgagttca ctatgcttga gtggtatcga ccgcactatg atatgtaccg gttgatgaac
     8461 gaggtggacg atctcttgca acaggtgctg gactgtccgg cagcagaaag cctttcttat
     8521 caacaagctt tcttgcgtta tctggaaatt gacccactct ctgccgacaa aacgcaactg
     8581 cgggaagtgg cagcgaaact ggatttgagc aacgttgccg atactgaaga agaccgcgac
     8641 acgcttctac aattgctgtt tacctttggt gtagagccaa atattggcaa agaaaaaccg
     8701 acctttgtgt accactttcc agccagccag gcatcactgg cgcaaatcag taccgaagat
     8761 catcgggtcg ctgaacgctt tgaggtttat tataaaggta ttgagctggc gaatggtttc
     8821 catgaattga cggatgcccg tgagcagcaa caacgctttg aacaagataa ccgtaagcgc
     8881 gcggcgcgcg gtttgccgca gcaccccatt gaccagaatc tgattgaagc cttgaaagtc
     8941 ggtatgcctg actgttccgg cgtggcatta ggcgttgatc gtctggtgat gttggcgctg
     9001 ggcgcggaga cactggctga agtcatcgcc tttagcgttg accgggcata attctgaaaa
     9061 attactgaat taaccgcgaa gaatggcgtg ataagcgcca ttctgtagca tatgttttca
     9121 tttttccgct aaaggccaca atttaagact ggatatttga tatcatccag gtatcaattc
     9181 tgtattggtt tttacgctgc ccgctcaatg ttgggcagtg cagttcagtg ggcgcgtttt
     9241 atacacgcgc tgaaatgaag gatgttttca tgcctcacac gataaaaaag atgagtctga
     9301 taggactcat attgatgatc tttacttccg tatttggatt tgccaatagc ccatcggctt
     9361 attacttaat gggttatagt gcgattccct tttatatatt ttctgcattg ttattcttta
     9421 ttccattcgc cttaatgatg gctgaaatgg gagctgctta tcgcaaagaa gaaggcggta
     9481 tctattcctg gatgaataat agtgtcggac cacgttttgc cttcattggt acgtttatgt
     9541 ggttttcctc ttatatcatc tggatggtga gtacctccgc gaaagtttgg gtaccgttct
     9601 caacattcct ctatggtagc gacatgaccc agcactggcg tattgccgga ctggagccta
     9661 cgcaggtggt tggtctgctg gcagtggcat ggatgattct ggtcaccgtc gttgcttcaa
     9721 aagggattaa taaaattgcc cgcattactg cggtgggcgg tattgcagta atgtgtctga
     9781 atttagtatt gctgttagta agcattacta ttttgttatt aaatggtggg catttcgcgc
     9841 aggatattaa tttccttgca tcaccgaacc caggttatca gtccggtctg gcaatgctat
     9901 cgtttgtggt atttgctatt tttgcctatg gtggaattga agcggttggt ggtctggtcg
     9961 ataaaacgga aaatccagaa aagaactttg ccaaaggtat tgtttttgcc gctattgtta
    10021 tttcaatcgg ttattcgctg gcaatatttt tatggggcgt cagcacaaac tggcagcagg
    10081 tattaagtaa tggttccgtt aacctcggca atattaccta tgtgctgatg aagagcctcg
    10141 gggtgacgct gggtaacgca ctgcatttgt cacctgaagc gtcattgtcg ctgggcgtat
    10201 ggtttgcgcg tattaccgga ctttcgatgt tcctcgctta taccggtgcg ttctttacgc
    10261 tttgctattc accgctgaaa gccatcatcc aggggacgcc gaaagcgttg tggccggaac
    10321 cgatgacgcg cctgaatgcg atggggatgc cgtctatcgc catgtggatg cagtgcgggt
    10381 tggttactgt cttcatcctg ctggtttcgt ttggtggcgg taccgcatcg gcgttcttta
    10441 acaagctgac gctgatggcg aacgtgtcta tgacgcttcc ttacctgttc ctcgcgctgg
    10501 ctttcccatt ctttaaagca cgtcaggatc tcgacagacc gtttgtgatt ttcaaaacgc
    10561 atatgtcggc aatgattgca acagtggttg tcgtactggt ggtgacattt gcgaacgtct
    10621 tcaccatcat tcaacctgtg gttgaagctg gagactggga cagcacattg tggatgattg
    10681 gcggccctgt cttcttctcg ctgttagcga tggcgattta ccagaactat tgcagtcgca
    10741 tggcgaacaa acctgagtta gctctcgact gatcatatga aatcctgcaa ctcatcgtct
    10801 ctccggcgat gagttgtctg gaaaggaaga cgttatggac gacgctagtc gggatccagt
    10861 gataaccgaa gatgaaatca gggagctgca attttctgcc ggggatgtgg cggaaataga
    10921 gcaaacggtc ctctcttttg tcgatacccg ccacacccgc aaagtagcga tggtggtggg
    10981 taacactatt aataccctca aagagcgcga tggtccgcgg tggggaaacc tgccggatat
    11041 ctattgcgct tatcttatta gatgtctggt ttttcgcgga gaattagtgg gatacggcga
    11101 tttgtttcgt atgcgctata gcgaaattaa gcgaccgatt atctcatgag tgcgcgcatg
    11161 tttatcttgt gctgcatctg gtttattgtg gcgttcctct ggataaccat cacttccgcg
    11221 ctggacaaag agtggatgat tgatggacgc gggattaata acgtctgcga tgtgctgatg
    11281 tatctcgaag atgacgatac ccgtgatgtc ggtgtgataa tgacgttacc gctctttttc
    11341 ccttttctct ggttcgcttt gtggcgaaaa aaacgcggct ggtttatgta cgccaccgcg
    11401 ctggctattt tcggctattg gctgtggcag ttttttctgc gctatcagtt ttgcttataa
    11461 gccggatggg ttcatccggc cacaaagacc ttacaaactt cccgcctgac gacctttgcc
    11521 cgcagaagtt aatgttctcc ccgtttgctt accattcagg ctttccaaac gcatctggaa
    11581 gggcgggaat ggcatatcaa taccgtgggc atggaatcct gccagaatta gctggtgaat
    11641 ctcatggcgt agtggcatac ggtgacccat ctcagcggcg taaatacgca gctcgaaaat
    11701 ttgaatcccc tgttgcagat ccaccaggaa gacttccggt gctgggttat cgattaccag
    11761 cgagcagcga cgcgctgcgg tgagcaggat ttccgtcact tcttcactgt tggcatcggc
    11821 aggggccgga atcgtcaaca ccacgcgcgt gaccgagtca gagagcgacc agttgataaa
    11881 ctgctcggta ataaacgcct tgttcggcac gataatttct ttgcggtccc agtcgctgat
    11941 ggtggtggcg cgggtgttaa ttttcgtcac actaccggta agatcgcgga tcgttaccgt
    12001 atcgccaatg cgaatcggtt tttcgaacag gataatcagg ccagagataa agttggcgaa
    12061 aatttcctgc aaaccaaaac cgagaccaac accgagcgcg gcgaccagcc actgcaattt
    12121 cgaccactca ataccaatca tcgagaagcc gaccagcccg ccaatcagca tcagcagata
    12181 tttggtgatg gtggtgatgg cgtagcccgt acccggcgtt aaatccaggt gctgcagaat
    12241 cgccagttcc agcagcgcgg gcaggttgcg caccagctgc gtggtgatga taaacaccag
    12301 aatggcaatc agcaccgcac cgagggtaat cggctccagg ctttctacgc cctgtaccgt
    12361 ggaagtgaca tcccacagcg aaatattttc gaggaagccg aaagcggaat ggatttctga
    12421 ccacagcaca atgaccgaaa gcagggcgat caacatcaaa attgagcgca ccagccgcaa
    12481 ggattgcgca ctgatggcat cgagatcgac ttcgctttca tcgacttcaa ttgctccttc
    12541 cgggctactg tgatgatgcg cttcctcttc gccacgcgca cgctgcgcta acatctctgc
    12601 ccggcgatgc ttcgcccggt caaacgccag cctgcggcgc tggatcagca tccagcggcg
    12661 gataacgtga taaaccacca gtagcaggaa ccagatggca accgaggttt caagcctcgc
    12721 taacagcgcc tgcgccgttg ccagataacc caccgccgac gccagaatgg caaccagcgg
    12781 tgcgccaatc atcatgttcc acagcatatg gttggtaatg ttgtcgccgc tgccttcttt
    12841 gttgagatac agcgggatcc cggccttttt cagactgaga gtgaccaccg ccagcgcacc
    12901 gcaaatgagg ataaagcaaa gccgtcccag cgaaccggag aattcacggt cgtcgaggtt
    12961 atcgaacatc atcagcgcca taatcagcgg cacaataagc ccgatgctca tcaggtagta
    13021 gcgcatccca cgggaaaccc gttcgcgcgg ccagccaaaa tgagcgataa acaagccgtt
    13081 cgggcgggca aaggtggcgc aaatcatcac tacccacagc agcggcacgg tggctgttac
    13141 gccatcacca atcgcgaccg ccagcggata aggccacgcc tcgcgcaagc cgtaacccag
    13201 cgtcatccac agcacaggca gcggtgacgc gacgagaatc gaccagaaaa gagtgcgtaa
    13261 cgtcagccag aagtgatcct gagtcacttt gccgactttc gccgccgaac gttcaagaaa
    13321 acgggtgaaa tagcggcgcg agtaaatact gcaaccgacc agaatcaacg cgccaagcag
    13381 cggcaaaatc gtctctttac tggtcagcat cattacactg gctttgccca actgactgaa
    13441 ggtgtccagc gaaatgagac gacgcagatc ctgggcgatt tccagcggcc aggcgatggt
    13501 catcgggcgc acgtcagagg tccagaacag atagcggtgc gtcgcttcat tcacctcttt
    13561 cagcgcatcc tccagttgcc cgttggagac tttcagcttg gtcagttcca gtagtagcgt
    13621 gtctccaccc tgcaacaatg agttcagcaa ctcacgctga gtgcgtagct gtgcttccag
    13681 aatacggttt tgctcggcgg tcagcggctg accgtcggcc tgatggattt gccgtagcag
    13741 cggctgttta tttagcagat cctcataacg taaccgttgc acacgcaact gcgccatttc
    13801 ggtatcaagc tgttgtggtt ttggcatttc cggtagccgt gccacctgcg cccgcaacgc
    13861 ttcgccgagc aggttggacg agcccagcca ttgcgactgt tcacgcagcg tattcaacgc
    13921 ctgccggacc tgtaacgtct ggctggcagc ctgacgctgt tgcgaggcaa cgagatccat
    13981 ccgctgcgcc tgttgattca gcgctgccga tagttcgcgg ttaattttga attgcgcgac
    14041 gatatctttc ggcaaatcgg cactgttttc tgccagtaat tcggtacttt ccagcgcccg
    14101 ctccgcctca agctgacgtt ggctgttcaa ctgattacgt aaggcctgca aatacgcatc
    14161 cagttgctgg ctctcttttt ccgccagctc tgagcgtaag cgcgctaatt cctgacggtt
    14221 attggcagac agctgcgcca gttccagttc atcgacgagc gctttcaggc gtgcggagtc
    14281 agactgcaac gcgaaatttt gtgcctgatt gagcggggaa ttgccggtaa gcgtccccag
    14341 gcggcgctcg atttcattta actgacggcg ggcatcggtt tgctgttgcg gcagttgatt
    14401 cagcgaatcg gcaatctcgc gggcgcgttc ctgctcttgc tgggcctgac ggcttttatc
    14461 cagcaactgg ctgctgacct ggagaatttc ctgattcagc gcgtcggtag acattcccgg
    14521 tgacacgctg cgcggttcgt cacgcatgtt gtttaattgt gcgcgcagag tagcggaaag
    14581 tttcgggtaa ttatcaatga cttcttgata ttgcttgatg cgctcaaggg aaccttttcg
    14641 ttcctcaagc gcatttaagg cggactggag cgcctctacg acttccggct gcgcgggttt
    14701 cgccgctttt gcctgctcca gttcttgtga gatttgtttg ctatcggggg ccgtcgcggc
    14761 gtacgccccc caactgaggc accaggccat cagaaaagtg ataatcaggc gcacgtcagc
    14821 gtttcctttg atggattaga cctggtcttt gttgtcgtca accaatgggt tggcgtcgtg
    14881 ttctgcttcg atctcttcag caggaagcgg ggccggttca gcgtctggtg taacaaaggt
    14941 ttcggtagat actgccagcg gctggccaat tttcgtgaca gacaggcttt ccagttgctc
    15001 aaccagattc actttacccg gtgcaaacag gttgataacg gtggaaccga gtttaaagcg
    15061 acccatttcc tggcctttca gcagtgccac agaaccgtcg ttttccccgg caggccaggt
    15121 ccagcgcttg atgatacctt cgcgcggcgg cgtaatggtg cccgcccaga ccgtctcaat
    15181 gctgcctaca atcgtcgccc cgaccagaat ctgcgccatt gggccaaatt cggtatcgaa
    15241 caggcagatc acgcgttcgt tacgggcaaa cagattcggc acgttctgag cggtgagatg
    15301 gttaacggaa aagagatcgc ccggcacgta gatcatctca cgcagaatac cgttgcacgg
    15361 catgtgtacg cggtggtagt cacgtgggga gaggtaagtg gtcacaaacg taccgttgcg
    15421 gaacaggtcc gccatcagat agttgccagc cagcagggct tcgaggctgt agttgtggcc
    15481 tttggcttgc aggattttat cttcttcgat tttacccaac tggctgataa cgccatcggc
    15541 aggcataacc agtacattcg gatcggtatc gattgggcgt acttcgtcac gcagcggacg
    15601 gacaaagaat tcgttaaagg tgcggtagct ggcggtgtcc ggcttttgcg cctctttcat
    15661 atcgaccttg tagtatttaa cgaacagatc gataaccagt tttgtcagcc atcctgcccg
    15721 cttgcttgcg ccccaacccg ccaggcgagt aagccatagt ttcggcagaa tgtactgtag
    15781 cgaaagttta aatgaattta acaaggtagc ctccaggcca ttgttttgtc gttcctgatc
    15841 cggcctacat gccggatcct gaaaaaaagg ggacgattct aacgacggtt agcttaattg
    15901 tcagtcatcc gtatcagaaa agtttttacg cgtttttacc tgcgccatgc tttccagaat
    15961 acggtgatag ttttcgaaac gggtttccgc gatttttcct tcctcaaccg cttcccggat
    16021 agcgcagccc ggatcggtat cgtgtttgca atcgcgatat ttacacagac ctaagtagtc
    16081 atggaattcg acaaagccct gagtgatttg ttccggctcc aggtgccaga ggccgaactc
    16141 acgcactcct ggggagtcaa tcacatcacc gccgtgcggg aagtgataca gccgagcggc
    16201 ggtcgtggtg tgctgaccga gacccgagtt gtcagagaca tcgttggtca ggatctcttt
    16261 ttgcagaccc agcaatgcgt tcagcaggct ggatttgcca acgccagact gcccggcaaa
    16321 aatgctgatg cgcccggtca acgcctcttc cagcggtttc agcccatcct gagtatgact
    16381 ggaaaccatc aatacgcgat aaccgatatt gcggtagata tccatctgct cgttgacgaa
    16441 tgccatgcct tcgtcgtcca gcaggtctat cttgttgagc acaataatcg gctcaatctg
    16501 caaggtttcg caggccacca ggtaacggtc gataatattg agcgacagct ccggcaaaat
    16561 ggcggagaca atgacaatct ggtcgatgtt ggcagcaata ggtttcacac cgtcgtaaaa
    16621 atccgggcgc gtcaacactg aggtgcgctc atgcaccgct tccacgatcc ctttgacatt
    16681 tacgccttcc gccgctggtt tacccggacg ccagactacg cggtcgccgg tcaccaacga
    16741 acggatggta cggcgaatat tgcagcggtg aacgtcgcca tcggcggatt ccacatcagc
    16801 gtgcatacca aagcggctga tgacgatacc ttcatcaggc tcgccaaaca gattgtcgtc
    16861 gtagtcgggc ttctccttag acgttttaag acgacgctgg tgattggcgt tcacgcggcg
    16921 ctgctggcct ttggagagtt tatttttact caatcgtaca gactcctggt cgcccctgat
    16981 gggcaaaaca tctatgatac acgcaattgt ggatcaatat agtcactgtg aatgggtgga
    17041 aaatagcatg agtgccaatg aaaacaacct gatttggatc gatcttgaga tgaccggtct
    17101 ggatcccgag cgcgatcgca ttattgagat tgccacgctg gtgaccgatg ccaacctgaa
    17161 tattctggca gaagggccga ccattgcagt acaccagtct gatgaacagc tggcgctgat
    17221 ggatgactgg aacgtgcgca cccataccgc cagcgggctg gtggagcgcg tgaaagcgag
    17281 cacgatgggc gatcgtgaag ctgaactggc aacgctcgaa ttcttaaaac agtgggtgcc
    17341 tgcgggaaaa tcgccgattt gcggtaacag catcggccag gatcgtcgct tcctgtttaa
    17401 atatatgccg gagctggaag cctacttcca ctaccgttat ctcgatgtca gcaccctgaa
    17461 agagctggcg cgccgctgga agccggaaat tcttgatggt tttaccaagc aggggacgca
    17521 tcaggcgatg gatgatatcc gtgagtcggt ggcagaactg gcttactacc gcgagcattt
    17581 tatcaagctg taaaattttt cctggtcacg taagcgccgg aatccacttg cggcggcgtg
    17641 attttgacgc taaattgaac gtcttgctga taatttcagc acttgagata aaaactcaaa
    17701 aaaaattttt taggggggtt gcagagggaa agatttctcg tataatgcgc ctcccgtaac
    17761 gacgcagaaa tgcgaaaatt acgaaagcaa aattaagtag tacgcgggaa tagctcagtt
    17821 ggtagagcac gaccttgcca aggtcggggt cgcgagttcg agtctcgttt cccgctccaa
    17881 aatttgaaaa gtgctgcaaa gcacagacca cccaatcgca gtatttcgaa ttgcgacaag
    17941 gcggcaactg aacgaatcct tgggaactta caaaagtaag tgactaaggt gagtgaaggc
    18001 agccaaggca gtagcggttc gaaagacgaa gattaagcgg gaatag
//
LOCUS       DAZFLD010000096        17706 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.097, whole genome shotgun sequence.
ACCESSION   DAZFLD010000096 DAZFLD010000000
VERSION     DAZFLD010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17706
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.097"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            2..1336
                     /gene="cdgI"
                     /locus_tag="ACHKBL_002728"
     CDS             2..1336
                     /gene="cdgI"
                     /locus_tag="ACHKBL_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012311644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HFO5819580.1"
                     /translation="MGTQKLKAQGFFIFSLLLTLILFCITTLYNENTNVKLIPQMNYL
                     MVVVALFFLNAVIFLFMLMKYFTNKQILPTLILSLAFLSGLIYLVETIVIIHKPINGS
                     TLIQTKSNDVSIFYIFRQLSFICLTSLALFCYGKDNILDNNKKKTGILLLALIPFLVF
                     PLLAHNLSSYNADYSLYVVDYCPDNHTATWGINYTKILVCLWAFLLFFIIMRTRLASE
                     LWPLIALLCLASLCCNLLLLTLDEYNYTIWYISRGIEVSSKLFVVSFLIYNIFQELQL
                     SSKLAVHDVLTNIYNRRYFFNSVESLLSRPVVKDFCVMLVDINQFKRINAQWGHRVGD
                     KVLVSIVDIIQQSIRPDDILARLEGEVFGLLFTELNSAQAKIIAERMRKNVELLTGFS
                     NRYDVPEQMTISIGTVFSTGDTRNISLVMTEADQALREAKSEGGNKVIIHHI"
     gene            1517..3007
                     /gene="dgcJ"
                     /locus_tag="ACHKBL_002729"
     CDS             1517..3007
                     /gene="dgcJ"
                     /locus_tag="ACHKBL_002729"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0043709 - cell adhesion involved in
                     single-species biofilm formation [Evidence IEA];
                     GO_process: GO:1902201 - negative regulation of
                     bacterial-type flagellum-dependent cell motility [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcJ"
                     /protein_id="HFO5819581.1"
                     /translation="MKLHHRMLRHFIAASVIVLTSSFLIFELVASDRAMSAYLRYIVQ
                     RADSSFLYDKYQNQSIAAHVMRALAAEQSEVSPEQRRAICEAFESANNTHGLNLTAHK
                     YPDLRGTLQTASTDCDTIVEAAALLPAFDQAVESNRHQDDYGSGLGMAEEKFHYYLDL
                     NDRYVYFYEPVNVEYFAMNNWSFLQSGSIGIDRKDIEKVFTGRTVLSSIYQDQRTKQN
                     VMSLLTPVYVAGQLKGIVLLDINKNNLRNIFYTHDRPLLWRFLNVTLTDTDSGRDIII
                     NQSEDNLFQYVSYVHDLPGGIRVSLSIDILYFITSSWKSVLFWILTALILLNMVRMHF
                     RLYQNVSRENISDAMTGLYNRKILTPELEQRLQKLVQSGSSVMFIAIDMDKLKQINDT
                     LGHQEGDLAITLLAQAIKQSIRKSDYAIRLGGDEFCIILVDSTPQIAAQLPERIEKRL
                     QHIAPQKEIGFSSGIYAMKENDTLHDAYKASDERLYVNKQNKNSRS"
     gene            3050..3553
                     /gene="yeaK"
                     /locus_tag="ACHKBL_002730"
     CDS             3050..3553
                     /gene="yeaK"
                     /locus_tag="ACHKBL_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mischarged aminoacyl-tRNA deacylase"
                     /protein_id="HFO5819582.1"
                     /translation="MTEMAKGSVTHQRLIALLSQEGADFRVVTHEAVGKCEAVSEIRG
                     TALGQGAKALVCKVKGNGVNQHVLAILAADQQADLSQLASHIGGLRASLASPAEVDEL
                     TGCVFGAIPPFSFHPKLKLVADPLLFERFDEIAFNAGMLDKSVILKTADYLRIAQPEL
                     VNFRRTA"
     gene            complement(3554..3658)
                     /gene="yoaI"
                     /locus_tag="ACHKBL_002731"
     CDS             complement(3554..3658)
                     /gene="yoaI"
                     /locus_tag="ACHKBL_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YoaI"
                     /protein_id="HFO5819583.1"
                     /translation="MNDQMFVETLIITSSFFAIAVVLVLSVLLIERTG"
     gene            3828..4274
                     /gene="yeaL"
                     /locus_tag="ACHKBL_002732"
     CDS             3828..4274
                     /gene="yeaL"
                     /locus_tag="ACHKBL_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HFO5819584.1"
                     /translation="MFDVTLLILLGLAALGFISHNTTVAVSILVLIIVRVTPLSTFFP
                     WIEKQGLSIGIIILTIGVMAPIASGTLPPSTLIHSFLNWKSLVAIAVGVIVSWLGGRG
                     VTLMGSQPQLVAGLLVGTVLGVALFRGVPVGPLIAAGLVSLIVGKQ"
     gene            complement(4231..5052)
                     /gene="nimR"
                     /locus_tag="ACHKBL_002733"
     CDS             complement(4231..5052)
                     /gene="nimR"
                     /locus_tag="ACHKBL_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NimR"
                     /protein_id="HFO5819585.1"
                     /translation="MMHRLNLNGYEPDRHHEAAVAFCIHAGTDELTSPVHQHRKGQLI
                     LALHGAITCTVENALWMVPPQYAVWIPGGVEHSNQVTANAELCFLFIEPSAVTMPTTC
                     CTLKISPLCRELILTLANRTTTQRAEPMTRRLIQVLFDELPQQPQQQLHLPVSSHPKI
                     RAMVEMMAKEPVEWGSLGQWARFFAMSERNLARLIVKETGVSFRQWRQQLQLIMALQG
                     LVKGDTVQKVAHTLGYDSTTAFITMFKKGLGQTPGRYIAGLTTVSPQSAKPDPRQ"
     gene            5149..6330
                     /gene="nimT"
                     /locus_tag="ACHKBL_002734"
     CDS             5149..6330
                     /gene="nimT"
                     /locus_tag="ACHKBL_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-nitroimidazole transporter"
                     /protein_id="HFO5819586.1"
                     /translation="MTCSTSLSGKNRIVLIAGILMIATTLRVTFTGAAPLLDTIRSAY
                     SLTTAQTGLLTTLPLLAFALISPLAAPVARRFGMERSLFAALLLICAGIAIRSLPSPY
                     LLFGGTAVIGGGIALGNVLLPGLIKRDFPHSVARLTGAYSLTMGAAAALGSTMVVPLA
                     LNGFGWQGALLMLMSFPLLALFLWLPQWRSQQHANLSTSRALHTRGIWRSPLAWQVTL
                     FLGINSLVYYVIIGWLPAILISHGYSEAQAGSLHGLLQLATAAPGLLIPLFLHHVKDQ
                     RGIAAFVALMCAVAAVGLCFMPAHAITWTLLFGFGSGATMILGLTFIGLRASSAHQAA
                     ALSGMAQSVGYLLAACGPPLMGKIHDANGDWSVPLMGVAILSLLMAIFGLCAGRDKEI
                     R"
     gene            6385..6732
                     /gene="yeaO"
                     /locus_tag="ACHKBL_002735"
     CDS             6385..6732
                     /gene="yeaO"
                     /locus_tag="ACHKBL_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HFO5819587.1"
                     /translation="MNIQCKRVYDPAEQSDGYRVLVDRLWPRGIKKTDLALDEWDKEI
                     TPSTELRKAFHGEVVDFATFREQYLAELAQHEQEGKRLANIAKKQPLTLLYSAKNTTQ
                     NHALVLADWLRSL"
     gene            complement(6754..7008)
                     /gene="yoaF"
                     /locus_tag="ACHKBL_002736"
     CDS             complement(6754..7008)
                     /gene="yoaF"
                     /locus_tag="ACHKBL_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing lipoprotein YoaF"
                     /protein_id="HFO5819588.1"
                     /translation="MKIISFVLPCLLVLAGCSTPSQPEAPKPPQIGMANPASVYCQQK
                     GGTLIPVQTAQGVSNNCKLPGGETIDEWALWRRDHPAGEK"
     gene            7191..8216
                     /gene="dgcP"
                     /locus_tag="ACHKBL_002737"
     CDS             7191..8216
                     /gene="dgcP"
                     /locus_tag="ACHKBL_002737"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcP"
                     /protein_id="HFO5819589.1"
                     /translation="MSDQIIARVSQSLAKEQSLESLVRQLLEMLEMVTDMESTYLTKV
                     DVEARLQHIMFARNSQKMHIPENFTVSWDYSLCKRAIDENCFFSDEVPDRWGDCIAAR
                     NLGITTFLSTPIHLPDGSFYGTLCAASSEKRQWSERAEQVLQLFAGLIAQYIQKEALV
                     EQLREANAALIAQSYTDSLTGLPNRRAIFENLTTLFSLARHLNHKIMIAFIDLDNFKL
                     INDRFGHNSGDLFLIQVGERLNTLQQNGEVIGRLGGDEFLVVSLNNENADISSLRERI
                     QQQIRGEYHLGDVDLYYPGASLGIVEVDPETTDADSALHAADIAMYQEKKHKQKTPFV
                     AHPALHS"
     gene            8249..8347
                     /gene="yoaK"
                     /locus_tag="ACHKBL_002738"
     CDS             8249..8347
                     /gene="yoaK"
                     /locus_tag="ACHKBL_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family small membrane protein"
                     /protein_id="HFO5819590.1"
                     /translation="MRIGIIFPVVIFITAVVFLAWFFIGGYAAPGA"
     gene            8350..8424
                     /gene="yoaJ"
                     /locus_tag="ACHKBL_002739"
     CDS             8350..8424
                     /gene="yoaJ"
                     /locus_tag="ACHKBL_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaJ"
                     /protein_id="HFO5819591.1"
                     /translation="MKKTTIIMMGVAIIVVLGTELGWW"
     gene            complement(8483..8731)
                     /gene="yeaQ"
                     /locus_tag="ACHKBL_002740"
     CDS             complement(8483..8731)
                     /gene="yeaQ"
                     /locus_tag="ACHKBL_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFO5819592.1"
                     /translation="MGILSWIIFGLIAGILAKWIMPGKDGGGFFMTILLGIVGAVVGG
                     WISTLFGFGKVDGFNFGSFVVAVIGAIVVLFIYRKIKS"
     gene            complement(8879..9061)
                     /gene="yoaG"
                     /locus_tag="ACHKBL_002741"
     CDS             complement(8879..9061)
                     /gene="yoaG"
                     /locus_tag="ACHKBL_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1869 domain-containing protein"
                     /protein_id="HFO5819593.1"
                     /translation="MGKATYTVTVTNNSNGVSVDYETETPMTLLVPEVAAEVIKDLVN
                     TVRSYDTENEHDVCGW"
     gene            complement(9065..9424)
                     /gene="yeaR"
                     /locus_tag="ACHKBL_002742"
     CDS             complement(9065..9424)
                     /gene="yeaR"
                     /locus_tag="ACHKBL_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1971 domain-containing protein YeaR"
                     /protein_id="HFO5819594.1"
                     /translation="MLQIPQNYIHTRSTPFWNKQTAPAGIFERHLDKGTRPGVYPRLS
                     VMHGAVKYLGYADEHSTEPDQVILIEAGQFAVFPPEKWHNIEAMTDDTYFNIDFFVAP
                     EVLMEGAQQRKVIHNGK"
     gene            complement(9597..10235)
                     /gene="leuE"
                     /locus_tag="ACHKBL_002743"
     CDS             complement(9597..10235)
                     /gene="leuE"
                     /locus_tag="ACHKBL_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine efflux protein LeuE"
                     /protein_id="HFO5819595.1"
                     /translation="MFAEYGVLNYWTYLVGAIFIVLVPGPNTLFVLKNSVSSGMKGGY
                     LAACGVFIGDAVLMFLAWAGVATLIKTTPILFNIVRYLGAFYLLYLGSKILYATLKGK
                     NNEAKSDEPQYGAIFKRALILSLTNPKAILFYVSFFVQFIDVNAPHTGISFFILATTL
                     ELVSFCYLSFLIISGAFVTQYIRTKKKLAKVGNSLIGLMFVGFAARLATLQS"
     gene            complement(10362..11285)
                     /gene="dmlR"
                     /locus_tag="ACHKBL_002744"
     CDS             complement(10362..11285)
                     /gene="dmlR"
                     /locus_tag="ACHKBL_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310535.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DmlR"
                     /protein_id="HFO5819596.1"
                     /translation="MNNLPLLNDLRVFMLVARRAGFAAVAEELGVSPAFVSKRIALLE
                     QTLNVVLLHRTTRRVTITEEGERIYEWAQRILQDVGQMMDELSDVRQVPQGMLRIISS
                     FGFGRQVVAPALSALAKAYPQLELRFDVEDRLVDLVNEGVDLDIRIGDDIAPNLIARK
                     LATNYRILCASPEFIAQHGAPKHLTDLSALPCLVIKERDHPFGVWQLRNKEGHHAIKV
                     TGPLSSNHGEIVHQWCLDGQGIALRSWWDVSENIASGHLVQVLPEYYQPANVWAVYVS
                     RLATSAKVRITVEFLRQYFAEHYPNFSLEHA"
     gene            11388..12473
                     /gene="dmlA"
                     /locus_tag="ACHKBL_002745"
     CDS             11388..12473
                     /gene="dmlA"
                     /locus_tag="ACHKBL_002745"
                     /EC_number="1.1.1.83"
                     /EC_number="1.1.1.85"
                     /EC_number="1.1.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional
                     D-malate/3-isopropylmalate/tartarate dehydrogenase"
                     /protein_id="HFO5819597.1"
                     /translation="MMKTMRIAAIPGDGIGKEVLPEGIRVLQAAAERWGFALSFEQME
                     WASCEYYSHHGKMMPDDWHEQLSRFDAIYFGAVGWPDTVPDHISLWGSLLKFRREFDQ
                     YVNLRPVRLFPGVPCPLAGKQPGDIDFYVVRENTEGEYSSLGGRVNEGTEHEVVIQES
                     VFTRRGVDRILRYAFELAQSRPRKTLTSATKSNGLAISMPYWDERVEAMAENYPEIRW
                     DKQHIDILCARFVMQPERFDVVVASNLFGDILSDLGPACTGTIGIAPSANLNPERTFP
                     SLFEPVHGSAPDIYGKKIANPIATIWAGAMMLDFLGNGDERFQQAHNGILAAIEEVID
                     HGPKTPDMKGNATTPQVADAICKIILR"
     gene            12724..14334
                     /gene="yeaV"
                     /locus_tag="ACHKBL_002746"
     CDS             12724..14334
                     /gene="yeaV"
                     /locus_tag="ACHKBL_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter YeaV"
                     /protein_id="HFO5819598.1"
                     /translation="MMSNVKKKDVPLISISLVAILFIAAALSLFPQQSADAANAIYTF
                     VTRTLGSAVQVLVLLAMGLVIYLATSKYGNIRLGEGKPEYSTLSWLFMFICAGLGSST
                     LYWGVAEWAYYYQTPGLNIAPRSQQALEFSVPYSFFHWGISAWATYTLASLIMAYHFH
                     VRKNKGLSLSGIIAAITGVRPQGPWGKLVDLMFLIATVGALTISLVVTAATFTRGLSA
                     LTGLPDNFTVQAFVILLSGGIFCLSSWIGINNGLQRLSKMVGWGAFLLPLLVLIVGPT
                     EFITNSIINAIGLTTQNFLQMSLFTDPLGDGSFTRNWTVFYWLWWISYTPGVAMFVTR
                     VSRGRKIKEVIWGLILGSTVGCWFFFGVMESYAIHQFINGVINVPQVLETLGGETAVQ
                     QVLMSLPAGKLFLAAYLGVMIIFLASHMDAVAYTMAATSTRNLQEGDDPDRGLRLFWC
                     VVITLIPLSILFTGASLETMKTTVVLTALPFLVILLVKVGGFIRWLKQDYADIPAHHV
                     EHYLPQTPVEVLEKTPVLPAGTVFKGDN"
     gene            14366..15490
                     /gene="yeaW"
                     /locus_tag="ACHKBL_002747"
     CDS             14366..15490
                     /gene="yeaW"
                     /locus_tag="ACHKBL_002747"
                     /EC_number="1.14.13.239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase, oxygenase subunit YeaW"
                     /protein_id="HFO5819599.1"
                     /translation="MSNLSPDFVLPENFCANPQEAWTIPARFYTDQNAFEHEKENVFA
                     KSWICVAHSSELANANDYVTREIIGESIVLVRGRDKVLRAFYNVCPHRGHQLLSGEGK
                     AKNVITCPYHAWAFKLDGNLAHARNCENVANFDSDKAQLVPVRLEEYAGFVFINMDPN
                     ATSVEDQLPGLGAKVLEACPEVHDLKLAARFTTRTPANWKNIVDNYLECYHCGPAHPG
                     FSDSVQVDRYWHTMHGNWTLQYGFAKPSEQSFKFEEGTDAAFHGFWLWPCTMLNVTPI
                     KGMMTVIYEFPVDSETTLQNYDIYFTNEELTDEQKSLIEWYRDVFRPEDLRLVESVQK
                     GLKSRGYRGQGRIMADSSGSGISEHGIAHFHNLLAQVFKD"
     gene            15546..16511
                     /gene="yeaX"
                     /locus_tag="ACHKBL_002748"
     CDS             15546..16511
                     /gene="yeaX"
                     /locus_tag="ACHKBL_002748"
                     /EC_number="1.14.13.239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase reductase subunit YeaX"
                     /protein_id="HFO5819600.1"
                     /translation="MSDYQMFEVQVSQVEPLTEQVKRFTLVATDGKPLPAFTGGSHII
                     VQMSDGDNQYSNAYSLLSSPHNTSSYQIAVRLEENSRGGSRFLHQQVKVGDRLTISTP
                     NNLFALIPSARKHLFIAGGIGITPFLSHLAELQYSDVDWQLHYCTRNPESCAFRDELV
                     QHPQAEKVHLHHSSTGTRLELARLLADIEPGTHVYTCGPEALNEAVRSEAARLNIAAD
                     TLHFEQFAIEDKTGDAFTLVLARSGKEFVVPEEMTILQVIENNKAAKVECLCREGVCG
                     TCETAILEGEADHRDQYFSDEERASQQSMLICCSRAKGKRLVLDL"
     gene            complement(16558..17673)
                     /gene="rnd"
                     /locus_tag="ACHKBL_002749"
     CDS             complement(16558..17673)
                     /gene="rnd"
                     /locus_tag="ACHKBL_002749"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0033890 - ribonuclease D activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HFO5819601.1"
                     /translation="MITTDDALASLCEAVRAFPAIALDTEFVRTRTYYPQLGLIQLFD
                     GEHLALIDPLGISDWSPLKAILRDPSITKFLHAGSEDLEVFLNVFGELPQPLIDTQIL
                     AAFCGRPMSWGFASMVEEYSGVTLDKSESRTDWLARPLTERQCEYAAADVWYLLPITA
                     KLMVETEASGWLPAALDECRLMQMRRQEVVAPEDAWRDITNAWQLRTRQLACLQLLAD
                     WRLRKARERDLAVNFVVREEHLWSVARYMPGSLGELDSLGLSGSEIRFHGKTLLALVE
                     KAQALPEEALPQPMLNLMDMPGYRKAFKAIKSLITDVSETHKISAELLASRRQINQLL
                     NWHWKLKPQNNLPELISGWRGELMAEALHNLLQEYPQ"
BASE COUNT         4391 a         4361 c         4266 g         4688 t
ORIGIN      
        1 tatgggtaca caaaaattaa aagctcaagg cttttttatt ttcagcttat tgctgacgtt
       61 aattttattt tgcattacta ccttatataa cgaaaacacg aatgtaaaac tcatcccaca
      121 gatgaattac ctgatggttg ttgtggcttt gtttttcctt aacgccgtca ttttcctttt
      181 catgttaatg aagtatttca ctaacaaaca aattttacca acactcattt taagccttgc
      241 atttttaagt ggccttatct atttagttga aaccattgta attatccata aaccaatcaa
      301 cggcagtaca ctgatccaga caaagtcgaa tgatgtttct attttctata ttttccgcca
      361 actcagtttc atttgtttaa cctcgctggc gctcttttgt tatgggaaag acaacatcct
      421 tgacaacaat aagaagaaaa ctggcattct gttgctggcg ctgattcctt ttttagtttt
      481 tccccttctg gcacacaatc tgagcagtta taacgctgac tattctttgt atgtcgtcga
      541 ttactgtccg gacaaccata ctgcgacctg gggaatcaac tatacaaaaa tattggtttg
      601 tttgtgggca tttttactgt tctttattat catgcgtaca cgattagcca gcgaactatg
      661 gccgttaata gcattattat gtctggcatc gctatgctgc aacttacttc tactgactct
      721 ggatgagtat aattacacca tctggtatat cagtcgcggg attgaagttt ccagtaaact
      781 gtttgttgtg tcttttctga tttataacat ttttcaggaa ctgcaactct ccagcaaact
      841 ggcagttcat gatgtactga ccaatattta taatcggcgc tactttttca acagcgtaga
      901 gtcattattg tcgcgacctg ttgttaagga cttttgtgtc atgctggttg atattaatca
      961 gttcaaacgc atcaatgccc aatggggaca tcgtgtgggt gataaagtgc tggtatcaat
     1021 tgtcgatatt atccagcaaa gcatccgccc cgatgatatt ttagcgcgac tggagggtga
     1081 ggtgtttggc ttgctattta ccgaactcaa tagtgcccag gcaaaaatca ttgcggaacg
     1141 tatgcgtaaa aatgtcgaac tcctgaccgg ctttagtaac agatatgatg ttcctgagca
     1201 aatgaccatc agtattggca cggttttttc aacgggtgac acacgtaata tctcgcttgt
     1261 catgacggaa gcagatcaag ccttacgcga agcgaaaagc gaggggggca acaaagtgat
     1321 tattcatcat atttaagtgc aaaaatattc agagccatgc tttttgcgtg gcttttgcat
     1381 acaatttatt actattaccc ctaaatttcc cctccccagc ggcgcagaga tgagtataat
     1441 tagcgcccct gtgccaggcc gcaatcgaac tttatctggt tttctcgttt cactaaccga
     1501 aggagtgcca tttatcatga aattgcacca tagaatgctc cggcatttta tcgccgcaag
     1561 tgtcattgtg ctgacatctt ccttccttat ttttgaactt gtcgccagcg acagagcaat
     1621 gagtgcctat ctgcgctata tcgtgcagag agcagactcc tcctttcttt atgataagta
     1681 tcagaatcag agtattgccg cgcatgtgat gcgcgctctc gctgctgagc agtcggaagt
     1741 gtcgccagaa cagcggcgcg ccatctgcga ggcttttgag tctgccaata atacccatgg
     1801 cttaaacctg actgcccata aatacccgga cttacgcggc acactacaaa ccgcatccac
     1861 tgactgcgac acaattgtgg aagctgcagc actattaccc gcttttgatc aggcagtgga
     1921 aagcaaccgc caccaggatg attacggttc aggtcttggg atggccgaag agaaatttca
     1981 ctattatctc gatctcaatg accgctatgt ctatttttat gaaccggtta atgttgagta
     2041 ctttgcgatg aataactggt ctttcctgca gtcaggaagt attggcatcg atcgcaaaga
     2101 tattgaaaag gtatttaccg ggcgtaccgt tttatcgagc atttaccagg atcagcgtac
     2161 taaacagaac gtgatgagtt tgctgacgcc ggtatatgtc gcagggcagc taaaagggat
     2221 tgtgctgctg gatattaaca aaaacaatct gcggaatatc ttttacactc atgaccgccc
     2281 tctcctctgg cgttttctca atgtcacgct aaccgatacc gattcggggc gcgacattat
     2341 catcaaccag agcgaagata atctgttcca gtatgtcagt tacgtccatg acttaccggg
     2401 cggcattcgt gtctcgttat ccattgatat tctttacttt atcacgtctt cgtggaaaag
     2461 cgttctgttc tggattttga cggcgttaat tttgctgaat atggtgcgga tgcacttccg
     2521 tttataccaa aacgtgtcgc gagaaaatat tagtgatgcg atgactggac tgtataatcg
     2581 caaaatttta acccctgaac tggagcagcg gttgcagaag ctggtgcaat ccggttcttc
     2641 ggtgatgttt attgcaattg acatggacaa gttaaagcaa ataaatgaca ccctcggtca
     2701 tcaggagggg gatttagcga ttacgttatt agctcaggcg attaaacaat cgattcgtaa
     2761 aagtgattat gccatccgac tcggtggcga tgaattctgc atcattcttg tcgattcgac
     2821 gccgcaaatt gcagcacaac tgcctgaacg tatcgaaaaa cgtctgcaac atatcgcgcc
     2881 gcagaaagag atcggcttct cttccggtat ttacgcgatg aaagaaaacg atacgttaca
     2941 tgatgcgtat aaagcttccg atgagcgttt atatgtcaat aagcagaaca aaaacagccg
     3001 ttcatgataa ccttctgtgg ttgtttgctt gtaatctcag gagcatgaaa tgactgaaat
     3061 ggctaaagga agcgtgacgc atcagcggtt aatcgcttta ttatcacaag aaggtgctga
     3121 cttccgcgtc gttacccatg aagcggtagg taaatgtgaa gcggtgtcag aaattcgtgg
     3181 caccgcttta ggccaggggg ctaaggcact ggtatgtaaa gtcaaaggca atggtgtaaa
     3241 tcaacatgtt ctggcgatcc tcgcggccga tcaacaggcc gatctgagcc aactcgccag
     3301 ccatataggc ggattacgcg cttcgttggc cagcccggcg gaagtcgatg aactcacagg
     3361 ctgtgtcttc ggcgcgatcc cccctttcag cttccatcca aaactcaaac tggttgccga
     3421 tcccctactc ttcgaacgat ttgatgaaat cgccttcaat gcaggcatgc tggataaatc
     3481 cgttattttg aaaaccgcgg attatttgcg cattgcgcaa ccagaactgg tcaatttccg
     3541 ccgcactgcg taactagcct gtccgttcga taagaagaac ggacaaaacc agcacaacag
     3601 caatggcaaa aaacgatgac gtgataatca gtgtctcgac gaacatttga tcgttcatag
     3661 tatgcccccg ggagatagtt gttttcacgt tattgttagc gtgcgcaaat ggcagtttga
     3721 tgacagttcg cctgattttt tatgcaaaaa acgtaaagat tttctacttc cttcctgcag
     3781 caagcgtaaa gtaagcaggc ttattattct ttggcaagga aaccacgatg tttgatgtca
     3841 ctttgctgat cctgctcggg ttagctgcgc tgggctttat cagtcataac accactgtcg
     3901 ccgtttcaat tctggtgtta atcattgtcc gcgtcacacc gttaagcacc tttttcccgt
     3961 ggattgaaaa acaggggcta agtatcggga taatcattct gacgattggc gttatggctc
     4021 ccatcgccag cgggacgcta ccgccctcaa ccctgattca ctcgtttctg aactggaaat
     4081 cgctggtggc aattgcagtc ggcgttattg tctcctggct gggggggcgc ggcgtgacgt
     4141 taatgggcag ccagccgcaa ctggtcgccg ggctgctggt gggcactgtt ttgggcgtgg
     4201 cgctgtttcg cggcgtaccg gttggcccgc ttattgccgc gggtctggtt tcgctgattg
     4261 tggggaaaca gtagttaatc ccgcaatata tcggcctggc gtttgcccca ggcctttctt
     4321 aaacatagta ataaaggccg ttgttgaatc ataccccagc gtatgagcaa ctttctgtac
     4381 agtatcgcct ttcactaatc cctgaagcgc cataatcagc tgtaactgct gacgccactg
     4441 gcgaaaactc acccccgtct ctttcactat cagtcgggca agattgcgtt cactcatcgc
     4501 aaaaaagcga gcccattgcc ccaatgaccc ccactcgacg ggctctttcg ccatcatctc
     4561 caccatcgcg cggattttag gatgagaaga aacgggtagg tgcagctgtt gttgtggctg
     4621 ctgagggagt tcatcaaaca ggacctgaat taatctgcgg gtcatcggct cagcacgctg
     4681 agtcgtcgtc ctgtttgcca gggttaaaat taattcccgg cataagggcg atattttcaa
     4741 tgtgcaacag gttgtcggca ttgtcactgc gctgggttcg ataaacagaa aacagagttc
     4801 agcgttagct gtcacttgat tgctatgttc cacccctccc ggtatccaaa ccgcatattg
     4861 tggcggcacc atccacaaag cattttccac cgtacaggta attgcaccat gtagtgccag
     4921 tatcaactgt cctttgcggt gctgatgtac aggactggtt aactcatccg ttccggcgtg
     4981 aatacaaaat gcgaccgcgg cttcatggtg tcggtcgggt tcatagccat tgagattcaa
     5041 cctgtgcatc attttgtccg aatttagcga taatttgtca ttttagcttg attcaacata
     5101 acaataaaaa cggtaaggta cagcctcgtt tgtaacaatg agaagcatat gacctgttca
     5161 acttcattaa gcggcaaaaa caggattgtc cttatcgctg gcattctgat gattgccaca
     5221 acattacgcg tcacctttac cggcgcagca ccgttactgg atacgattcg ttccgcttac
     5281 tcgctgacga cggcgcaaac cggcttattg accaccctgc cattattagc ctttgcgcta
     5341 atctcacctt tggctgcccc ggtggcgcga cgttttggta tggaacgtag cctgtttgcc
     5401 gcgttacttt tgatctgtgc tggtatcgca attcgctctc tcccttcgcc ttacttatta
     5461 tttggcggta cagcggtcat tggcggtggg attgcattag gcaatgtctt actgccagga
     5521 ttaattaaac gcgatttccc tcattccgtc gccagactta ccggcgcata ttccctgaca
     5581 atgggagctg cagcggcact gggatcgact atggtcgtgc cgctggcttt gaacggtttt
     5641 ggctggcaag gcgcgttgct catgctgatg tcttttcctc tgctggctct ttttttatgg
     5701 ctgccacagt ggcgaagtca acaacatgca aatttgagta cctcacgcgc cttacatact
     5761 cggggtatct ggcgttcgcc gcttgcctgg caggtaacat tgtttcttgg gatcaactca
     5821 ctggtctatt acgtgattat tggctggctt ccggcgattc tcatcagtca cggctatagc
     5881 gaagcacagg cgggttcact gcatggtttg ctgcaactag ccacagcagc tcccggtttg
     5941 ctgatcccac ttttcttaca tcatgtgaaa gatcagcgtg gtattgcagc gttcgttgcc
     6001 ttgatgtgcg cagtggccgc ggttgggctc tgctttatgc cagcacacgc gatcacctgg
     6061 actctgcttt tcggttttgg ttccggcgca acaatgatac tggggttgac gttcattggt
     6121 ctgcgggcaa gttctgcgca tcaggcggcg gcactctcgg ggatggcaca atccgtcggg
     6181 tatttgctgg cagcctgtgg gccgccgctg atgggtaaaa tacacgatgc taacggtgac
     6241 tggtccgtac cactgatggg tgttgcaata ctttcactac tgatggcgat tttcggactt
     6301 tgcgccggga gagacaaaga aattcgctaa tatccggtgc tatagtgacg tggcaaatca
     6361 tgcgtgaaag ggagaacaaa tacgatgaat attcagtgca aacgcgttta tgatccggct
     6421 gaacagagcg atggttatcg cgtactggtc gaccgcctct ggccacgcgg tatcaaaaaa
     6481 accgatttag cccttgatga atgggataaa gaaatcacgc cgtcaacgga actgcgcaaa
     6541 gcctttcacg gcgaagtcgt cgattttgca acctttcgcg agcaatatct tgcagaactg
     6601 gcgcaacacg agcaagaagg aaagcggctg gcgaacatcg ccaaaaaaca gccgctgacc
     6661 ctgctctact cagcaaaaaa caccacgcag aaccatgcgc tggtgctggc agactggcta
     6721 cgtagcttgt gattttagta cgccatccgg cagttatttt tcaccagccg gatggtcccg
     6781 tcgccacaat gcccattcat caatcgtttc accgcccggt aatttgcaat tgttgctgac
     6841 cccttgcgct gtctgcacag gaatgagcgt cccgcccttc tgctggcaat agaccgacgc
     6901 cggatttgcc ataccaatct gcggcggttt aggtgcttct ggctgagaag gggttgaaca
     6961 accagccagg accagcaagc aaggcagaac aaaactgata attttcattt attgatctca
     7021 catatttatc cagacttaga gtatcgcggt atcgttttgt tttgcagcac tatttttatt
     7081 acattcactc aaatcatatt acgccttgtt tcatctttgt tgatgatgtt ttatcatgcc
     7141 tgcaaagatt aaataatcag catttacccg ccgtatcctg gagttgtttc gtgtcagatc
     7201 agattatcgc ccgcgtctcg caatcccttg ccaaagaaca gtcactggaa agcctggtcc
     7261 gacagcttct ggagatgctg gaaatggtca ctgatatgga atcaacctac ctgaccaaag
     7321 tggatgtcga agcgcgcctg cagcatataa tgtttgcccg taacagccag aaaatgcaca
     7381 tcccggagaa ttttaccgtc tcgtgggatt actcgttatg caaacgcgcc attgatgaaa
     7441 actgcttttt cagcgatgaa gtccctgacc gttggggcga ctgtattgcg gcacgcaatc
     7501 ttggcatcac cacatttctg agcacgccaa ttcacttacc ggatggatca ttctatggca
     7561 cgctttgcgc cgccagcagt gagaagcgcc agtggagtga acgcgcggaa caggttttac
     7621 agttattcgc cggactgatt gcacaatata ttcaaaaaga ggcactggtt gagcagctgc
     7681 gcgaagccaa tgctgcgctg attgcgcaat cgtataccga ctcgttaacc gggctaccga
     7741 atcggcgggc gatttttgaa aatctgacga cgctgttttc tctcgcccga catcttaacc
     7801 ataagataat gatcgcgttt atcgatctgg ataacttcaa attaatcaac gatcgttttg
     7861 gtcataatag tggtgatttg ttcctcattc aggttggcga gcgccttaat acgctccagc
     7921 aaaatggcga agttattggt cgtctcggcg gtgatgagtt tttggttgtt tcactgaaca
     7981 acgagaatgc ggatatttcg tctctgcgag aacgtattca acagcaaata cgtggagaat
     8041 atcacttagg tgacgttgat ttgtattatc ccggtgccag tcttggcata gtagaagtcg
     8101 atcctgaaac gaccgatgca gacagtgccc tgcatgctgc cgatatcgcg atgtatcagg
     8161 agaaaaaaca caaacagaaa acaccttttg tcgcgcatcc agcgctacat tcctgaggcg
     8221 tattcacatc cttttgattg gtgataacat gcgaatcggt attatttttc cggttgtaat
     8281 ctttattaca gcggtcgtat ttttagcatg gttttttatt ggcggctatg ctgccccggg
     8341 agcataaaga tgaaaaaaac aacgattatt atgatgggtg tggcgattat tgtcgtactc
     8401 ggcactgagc tgggatggtg gtaacgtcac atctagaaaa tagcaaaggc tgcctgtgtg
     8461 cagcctttgt gcaatttacg cgttaacttt taatctttct gtagataaac agcacgacaa
     8521 tcgcgccaat aacggcaacc acgaagctgc caaaattgaa gccatcgact ttaccaaagc
     8581 caaacagcgt gctgatccat ccgccgacta cggcaccgac tatccccagc aggatagtca
     8641 taaagaatcc accaccatct ttacctggca tgatccactt cgccagaata ccggcaataa
     8701 gcccaaaaat aatccatgac agaatgccca ttgtttcctc acttatctgt tttgcattag
     8761 cgggttagtc gctgataaaa agcatagcac aacatcgtga gggcaagatt tgtgacgagc
     8821 atcacggagg atttttttgc gatggcgcag aaattgcgcc atcaacgatc agtgataatt
     8881 accaaccaca aacatcatgt tcgttttccg tgtcataaga acgcacggta ttcaccagat
     8941 cttttatcac ttcagccgcc acttctggca ccagcaaagt catcggcgtc tctgtttcat
     9001 aatcgacaga aacgccattg ctgttattgg tgacggtcac ggtatacgtt gctttgccca
     9061 tgattcattt cccgttatga atgactttcc gttgttgcgc accttccatc aggacttcag
     9121 gagcaacgaa gaagtcaatg ttgaaataag tgtcgtcagt catggcttca atgttgtgcc
     9181 acttttctgg agggaacaca gcaaactgcc ccgcttcgat aaggatcact tgatcaggct
     9241 ctgtactgtg ttcatcagcg tagccgagat atttgaccgc cccatgcata acggaaaggc
     9301 gtgggtaaac ccccgggcgc gttcctttat caagatgacg ttcgaatatt ccggcaggcg
     9361 cagtttgttt attccagaaa ggcgttgagc gcgtatgaat ataattctgt gggatttgca
     9421 gcatcctttt ccctccttcg gtgaatgcgc tgaaaacggt ttatttcagc cgtttcaggg
     9481 tacgcctgat aatttgcatt ttaaatacca tttattggtt actatttagc accgtatcag
     9541 agaagaatca gggaggatta caggtgggaa gcccgcgaca acgcgggctg aatgcatcag
     9601 gactggagcg tcgccagtcg ggcggcgaaa cccacaaaca tcaaaccaat cagtgagttg
     9661 cccactttag ccagtttctt tttggtacgt atgtactgcg tgacaaaagc accagaaata
     9721 atcaggaagc tcaaatagca gaaactcacc agttccagcg tcgtcgccag aataaagaat
     9781 gaaattcccg tatgtggggc attaacatcg ataaactgta cgaaaaacga cacatagaac
     9841 aaaatggctt tcggattagt caggctcaaa attaacgcgc gtttaaaaat ggcaccgtat
     9901 tggggctcat cggatttggc ctcgttattt ttacccttca gggtcgcgta gagaatttta
     9961 ctccccagat agagcaaata aaacgcacca agataacgta caatgttgaa taatatcggg
    10021 gtggtcttaa ttaatgtcgc cactccagcc catgccagaa acatcaatac cgcatcgcca
    10081 ataaatacac cgcaggccgc aagataaccg cctttcatac cgctactgac gctatttttg
    10141 agtacaaaca gggtatttgg ccctggcacc aacacaataa aaatggcccc aaccagatag
    10201 gtccagtaat tcagaacccc gtattcagcg aacacattaa cctctttaat tatctttcga
    10261 tcatgcgcga ttaaaggtga atatgctaac caatctgtag cggcttagaa aggagaaaat
    10321 caggttttaa cctgatatca acccgataat tgaatcatta atcaggcatg ctccagtgaa
    10381 aaattcgggt agtgctcggc aaaatactgg cgtaaaaatt ctaccgttat ccgtactttc
    10441 gctgacgtcg ccagccttga aacataaacg gcccagacat tcgctggctg gtaatattcc
    10501 ggtagcactt gtactaaatg accactggca atgttttcgc taacatccca ccaggagcgc
    10561 agagcaatcc cctgcccatc gagacaccac tgatggacaa tttctccatg attagacgac
    10621 agtgggccgg taactttaat cgcatggtgg ccttctttat tgcgtaattg ccagacacca
    10681 aaaggatgat cgcgctcttt aatgaccagg cacggcaatg cagaaagatc ggttaaatgt
    10741 ttcggcgcgc catgttgcgc aataaattcc ggtgacgcgc agagaatgcg ataattggtc
    10801 gccagtttgc gggcgattaa attaggggcg atgtcatcgc caatgcgaat atcgagatcg
    10861 acaccttcat tgaccaaatc gaccaaccgg tcttccacat caaaacgtaa ttccagttgc
    10921 ggatacgcct tcgccagcgc tgataacgcc ggggccacca cctgtcgtcc aaaaccaaag
    10981 ctgctgataa tacgcagcat cccctgtggc acctgacgca cgtcagaaag ttcgtccatc
    11041 atctgaccga catcctgcaa aatccgctgc gcccattcat aaatccgctc tccttcttcg
    11101 gtaatggtga cgcggcgggt ggtgcggtgt aacaacacca cgtttagcgt ttgctccagc
    11161 aaggcgatgc gcttgctgac gaacgccgga gaaacgccca gttcttccgc cacggcggca
    11221 aaaccagccc ggcgagccac cagcataaag acgcgcaaat cgttcagcag cggtaaatta
    11281 ttcatgattc gtgttttatg tttcaccagt tacagggatt aattcctttt cagtcaatta
    11341 taggatggtg atgttgtcaa ttttgatggt caggaagtga gaacccaatg atgaaaacga
    11401 tgcgtattgc tgcgatcccg ggagacggga ttggcaaaga agtccttcct gaagggattc
    11461 gcgtgttaca ggctgccgct gagcgctggg gcttcgcctt gagttttgag caaatggagt
    11521 gggcgagctg cgagtattac agccatcacg gtaaaatgat gccggacgac tggcatgagc
    11581 aacttagccg tttcgacgcc atctattttg gtgccgtcgg ctggccggac accgttccgg
    11641 accatatttc gttgtggggt tcgctgctga aatttcgtcg tgaattcgac cagtacgtca
    11701 acctgcgccc ggttcgtctc tttcctggcg ttccctgccc gctggcggga aaacagcctg
    11761 gcgacatcga tttttacgtg gtcagggaaa acaccgaagg cgaatattcc tcgctcggcg
    11821 gcagagtgaa tgaaggtaca gagcatgaag tcgtcattca ggaatcggta tttactcgtc
    11881 gtggtgtcga tcgtattttg cgctatgcct ttgaacttgc gcaaagccgc ccacgtaaga
    11941 cgctaacttc tgccactaaa tcgaacggtt tagccatcag catgccgtac tgggatgagc
    12001 gagtggaagc aatggctgag aattacccgg agatccgctg ggacaagcag catattgaca
    12061 ttctctgcgc acgttttgtg atgcaaccgg aacgcttcga tgtggtagtg gcgtccaatt
    12121 tgtttggcga tatcctttcc gatcttggcc cggcctgcac cggcaccatt ggcattgccc
    12181 catccgccaa cctgaatccg gaacgcactt tcccgtcgct cttcgagcct gtccacggtt
    12241 ccgcgccgga tatctacggg aaaaaaattg ctaaccctat cgccacaatt tgggccggtg
    12301 caatgatgct cgattttctc ggcaatggag atgagcgttt ccagcaagcg cataacggta
    12361 ttctggccgc gattgaagaa gtgattgatc acgggccgaa aacacctgat atgaaaggca
    12421 atgccaccac gccacaggtt gccgacgcga tttgcaaaat tattttgcgt taaggtcaaa
    12481 ccagtttatt tgaaccgcgt cactgacgcg gtttttttat tcgttctttg cagtaaataa
    12541 cctgcgtcat ttcacctttt attgtttccg tttcgtgttt tatggctttc cgtattctta
    12601 attgtttaat ttatgtaaca tgcaaataat caatttgtta atcacgacat acaactcatt
    12661 accctgcaaa taattacaac tggcaaaagc cagagacggg cttttatgcc caggagatga
    12721 ctcatgatga gcaatgttaa gaaaaaagat gtgccgctga taagcatcag cctggtggcc
    12781 attcttttca tcgcagctgc attaagcctt ttcccacaac aatcggccga cgcggccaac
    12841 gcaatataca cttttgttac gcgtacgtta ggttccgccg tacaggtatt ggttttgctg
    12901 gcaatgggac tggtgattta tttagccacc agtaaatacg gcaatattcg tcttggcgaa
    12961 ggaaaaccgg aatacagcac gctctcatgg ctgtttatgt ttatttgtgc cggtttaggt
    13021 tcttctacgc tttattgggg ggttgctgaa tgggcctatt attatcaaac gcctggatta
    13081 aatatcgcac cgcgttcaca acaggcactc gaatttagcg ttccctactc cttcttccac
    13141 tggggcatca gcgcctgggc aacttatacg ctggcctcat taatcatggc ttatcacttt
    13201 catgtgcgga aaaataaagg gctgagcctt tccggcatta ttgctgctat taccggcgtt
    13261 cgcccgcaag gcccgtgggg aaaactggtc gatttgatgt tcctgatcgc cactgtcggc
    13321 gcactgacca tttcccttgt tgtcaccgca gcaaccttta cccgtgggct ttccgcgctg
    13381 accggtttac ccgataactt taccgtacag gcatttgtga tcctgctttc cggcggcatt
    13441 ttttgcctaa gctcgtggat tggtatcaac aacggtttgc aacgtctaag caaaatggtt
    13501 ggctggggcg cgttcctgct gccattactg gtgctgattg tcggtccaac cgaatttatt
    13561 accaacagca tcatcaatgc catcggcctg accacgcaaa acttcctgca aatgagctta
    13621 ttcaccgatc cgcttggcga tggttcattt acccgcaact ggaccgtttt ctactggctg
    13681 tggtggatct catacacgcc tggcgtagca atgtttgtca cccgcgtttc ccgcggtcgt
    13741 aagattaaag aagttatctg gggactgatc ctcggcagca ccgtcggttg ctggttcttc
    13801 ttcggcgtaa tggaaagcta tgccattcac cagtttatca atggcgtaat caacgtccca
    13861 caggtgctgg aaacactggg cggcgagaca gctgtgcagc aagttctgat gtcgttgcca
    13921 gccggtaaat tgttcctcgc cgcatacctg ggcgtgatga ttattttcct tgcctcgcat
    13981 atggatgcgg tggcctacac catggcggca accagtacgc gtaatctcca ggaaggtgac
    14041 gatcctgacc gtgggctgcg tcttttctgg tgcgtggtaa ttaccctgat cccgctttca
    14101 attcttttca ctggcgcgtc gctggaaacg atgaaaacca ccgtcgtact cacagccctt
    14161 ccgttcctcg tcattttact ggtgaaagtc ggcggattta ttcgctggct gaaacaggat
    14221 tacgccgaca ttccggctca tcatgttgaa cattatctcc cgcagacacc ggttgaagtc
    14281 ctggaaaaaa cgccagtgct ccctgcggga accgtattca aaggcgacaa ctgagcgcca
    14341 tcatcctaac gataaaggta tccctatgag caatctgagc cctgactttg tactacccga
    14401 aaatttttgc gctaacccgc aagaggcgtg gaccattcct gcccgttttt ataccgatca
    14461 gaacgccttt gaacacgaaa aagagaacgt cttcgccaaa agctggattt gcgtcgctca
    14521 cagcagcgaa ctggcgaatg ccaatgatta tgtgacgcgt gagatcattg gcgaaagcat
    14581 tgtgctggta cgcggtcgtg ataaggtttt gcgcgcgttc tataacgtgt gtccgcaccg
    14641 tggtcatcag ttgttgagcg gcgaagggaa agcgaaaaat gtgattacct gcccgtatca
    14701 cgcatgggca ttcaaactcg atggcaacct ggcccatgca cgtaactgcg aaaacgtcgc
    14761 caatttcgat agcgacaaag cgcaactggt tccggtgcgt ctggaagaat atgccggatt
    14821 cgtcttcatc aacatggacc ctaacgccac cagcgtagaa gatcagttac ccggtctggg
    14881 cgcgaaagtg ctggaagcct gcccggaagt ccacgatctg aaactggcgg cccgctttac
    14941 cacccgcacg cctgccaact ggaagaacat tgtcgataac tatctcgagt gctatcactg
    15001 tggtccggcg catccaggtt tctccgactc cgtacaggtt gatcgttact ggcacaccat
    15061 gcacggtaac tggacgctgc aatacggttt cgccaaaccg tcagaacagt ctttcaaatt
    15121 cgaagagggc acggatgcgg cattccacgg tttctggctg tggccgtgca cgatgcttaa
    15181 cgtcacaccg atcaaaggga tgatgacggt catttatgaa ttcccggtgg attctgaaac
    15241 taccctgcaa aactacgata tttacttcac caatgaagag ttaaccgacg agcaaaaatc
    15301 gctgattgag tggtatcgcg atgtgttccg tccggaagat ttacgtctgg ttgaaagcgt
    15361 acagaaaggg ctgaaatcgc gtggctatcg tggtcagggg cgcatcatgg ccgacagtag
    15421 cggtagcgga atttccgaac atggtatcgc ccatttccat aatctgctgg cgcaggtgtt
    15481 taaggactaa tgacatcggc ggcggtattt tccgccgctg ggctgatttt tgatggagta
    15541 cagcaatgtc agactatcaa atgtttgaag tacaggtgag tcaggtggaa ccgcttaccg
    15601 aacaggtgaa acgcttcacg ctggtggcaa ccgatggcaa accgctgcct gcgttcaccg
    15661 gaggaagtca catcatagtg cagatgagcg atggtgataa ccagtacagc aatgcgtatt
    15721 cactactgag ttcaccgcat aacacctcta gttatcagat tgccgttcga ctggaggaaa
    15781 actcgcgcgg cggttcccgc tttttgcatc agcaggtaaa agtgggcgat cggttaacga
    15841 tttcaacgcc taataacctg tttgcgctaa ttccctcggc cagaaagcat ctgtttatcg
    15901 cgggcggtat tggtatcacc cctttcctgt cgcacctggc agagttgcaa tacagcgacg
    15961 tcgactggca gctacattac tgcacgcgaa atccagaaag ttgcgcattt cgtgatgagc
    16021 tagtccagca tccacaggct gagaaagttc acttacatca ctcatcaacc ggaacacgac
    16081 tggaattagc gcgattattg gcggatatcg aacctggcac acatgtttat acctgtggcc
    16141 ccgaggcgct aaatgaagcg gtaagaagtg aagctgcgcg tctgaacatc gccgccgata
    16201 cgctgcactt tgagcaattt gctatcgaag acaaaaccgg cgatgcattt accctggtgc
    16261 ttgcccgttc cggaaaagag tttgtggtgc cggaagagat gactattttg caggttattg
    16321 aaaataataa agcagcgaaa gtggaatgtt tatgtcgtga aggggtatgc ggaacctgcg
    16381 aaacggcaat actggaaggt gaagctgacc atcgggatca gtattttagc gatgaagagc
    16441 gtgccagcca gcaaagtatg ttgatctgtt gttcgcgcgc gaagggtaaa cgcctggtgt
    16501 tggatttgta gtttgccgaa gccggactga gcgcgcccag tccggcttcg gaagatttta
    16561 ctgcggatat tcctgcaata aattgtgtaa tgcttccgcc atcagctcac cacgccagcc
    16621 ggaaatcagc tccggcaaat tgttctgcgg tttcagtttc cagtgccagt tcagcagttg
    16681 gttgatttgc cgacgcgatg ccagcaattc ggcgctgatc ttgtgcgttt cgctcacgtc
    16741 agtaatcagc gacttaatcg ctttaaacgc tttacgataa cccggcatgt ccatcaggtt
    16801 aagcatcggc tgcggtaagg cctcttccgg caatgcctgc gctttttcca ccagcgccag
    16861 cagcgtttta ccgtgaaagc ggatttcgct accggataag cccaggctgt ccagttcgcc
    16921 taaacttccc ggcatataac gcgctacgga ccacaaatgc tcttcacgca cgacaaagtt
    16981 cactgccaga tcgcgctccc gcgccttgcg cagtcgccag tcggctaaca gttgcagaca
    17041 ggccagttgg cgtgtgcgta actgccaggc attggtgata tcacgccagg catcttccgg
    17101 agcaacgact tcctgacgac gcatttgcat caggcggcat tcatccagcg ccgcaggtag
    17161 ccagccggag gcctccgttt ctaccataag ctttgcggtg atcggtaaca gataccagac
    17221 atccgccgct gcgtattcac actgacgttc ggtcagcggt ctggccagcc agtcggtgcg
    17281 cgattcactc ttgtccagcg taacgccgga atactcttcc accatggaag cgaaacccca
    17341 tgacatcggg cgtccgcaga aggcagcaag gatttgcgtg tcaatcaagg gttgtggtaa
    17401 ttcgccaaag acattgagga acacttccag atcttcactg cccgcatgga gaaattttgt
    17461 gatggacgga tcgcgcagga tcgctttcag cggtgaccag tcagagatcc cgaggggatc
    17521 aattagcgcc agatgctcac catcaaaaag ttgaattaga cccagttgcg ggtaataggt
    17581 gcgcgtacga acaaattcag tatccagggc tatcgccgga aaggcacgga cggcttcaca
    17641 caaagaagcc agcgcatcgt ccgtggtaat catttggtaa ttcaaattgt attctcttta
    17701 gtgggc
//
LOCUS       DAZFLD010000097        17689 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.098, whole genome shotgun sequence.
ACCESSION   DAZFLD010000097 DAZFLD010000000
VERSION     DAZFLD010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17689
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.098"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(45..698)
                     /gene="fdnI"
                     /locus_tag="ACHKBL_002750"
     CDS             complement(45..698)
                     /gene="fdnI"
                     /locus_tag="ACHKBL_002750"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit gamma"
                     /protein_id="HFO5819602.1"
                     /translation="MSKSKMIVRTKFIDRACHWTVVICFFLVALSGISFFFPTLQWLT
                     QTFGTPQMGRILHPFFGIAIFVALMFMFVRFVHHNIPDKKDIPWLLNIVEVLKGNEHK
                     VADVGKYNAGQKMMFWSIMSMIFVLLVTGVIIWRPYFAQYFPMQVVRYSLLIHAAAGI
                     ILIHAILIHMYMAFWVKGSIKGMIEGKVSRRWAKKHHPRWYREIEKAEAKKESEEGI"
     gene            complement(691..1575)
                     /gene="fdnH"
                     /locus_tag="ACHKBL_002751"
     CDS             complement(691..1575)
                     /gene="fdnH"
                     /locus_tag="ACHKBL_002751"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase N subunit beta"
                     /protein_id="HFO5819603.1"
                     /translation="MAMETQDIIKRSATNSITPPSQVRDYKAEVAKLIDVSTCIGCKA
                     CQVACSEWNDIRDEVGHCVGVYDNPADLSAKSWTVMRFSETEQNGKLEWLIRKDGCMH
                     CEDPGCLKACPSAGAIIQYANGIVDFQSENCIGCGYCIAGCPFNIPRLNKEDNRVYKC
                     TLCVDRVSVGQEPACVKTCPTGAIHFGTKKEMLELAEQRVAKLKARGYEHAGVYNPEG
                     VGGTHVMYVLHHADQPELYHGLPKDPKIDTSVNLWKGALKPLAAAGFIATFAGLIFHY
                     IGIGPNKEVDDDEEEHHE"
     gene            complement(1588..4635)
                     /gene="fdnG"
                     /locus_tag="ACHKBL_002752"
     CDS             complement(1588..4635)
                     /gene="fdnG"
                     /locus_tag="ACHKBL_002752"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0047111 - formate dehydrogenase
                     (cytochrome-c-553) activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(4048..4050),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HFO5819604.1"
                     /translation="MDVSRRQFFKICAGGMAGTTVAALGFAPKQALAQARNYKLLRAK
                     EIRNTCTYCSVGCGLLMYSLGDGAKNAREAIYHIEGDPDHPVSRGALCPKGAGLLDYV
                     NSENRLRYPEYRAPGSDKWQRISWEEAFSRIAKLMKADRDANFIEKNEQGVTVNRWLS
                     TGMLCASGASNETGMLTQKFARSLGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANVVMVMGGNAAEAHPVGFRWAMEAKNNNDATLIVVDPRFTRTASVADIYAPIR
                     SGTDITFLSGVLRYLIENNKINAEYVKHYTNASLLVRDDFAFEDGLFSGYDAEKRQYD
                     KSSWNYQFDENGYAKRDETLTHPRCVWNLLKAHVSRYTPDVVENICGTPKADFLKVCE
                     VLASTSAPDRTTTFLYALGWTQHTVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSTSLPGYLTLPSEKQVDLQSYLEANTPKATLADQVNYWSNYPKFFVSL
                     MKSFYGDAAQKENNWGYDWLPKWDQTYDVIKYFNMMDEGKVTGYFCQGFNPVASFPDK
                     NKVVSCLSKLKYMVVIDPLVTETSTFWQNHGESNDVDPASIQTEVFRLPSTCFAEEDG
                     SIANSGRWLQWHWKGQDAPGEARNDGEILAGIYHHLRELYQAEGGKGVEPLMKMSWNY
                     KQPHEPQSDEVAKENNGYALEDLYDANGVLIAKKGQLLSSFAHLRDDGTTASSCWIYT
                     GSWTEQGNQMANRDNSDPSGLGNTLGWAWAWPLNRRVLYNRASADINGKPWDPKRMLI
                     QWNGSKWTGNDIPDFGNAAPGTPTGPFIMQPEGMGRLFAINKMAEGPFPEHYEPIETP
                     LGTNPLHPNVVSNPVVRLYEQDALRMGKKEQFPYVGTTYRLTEHFHTWTKHALLNAIA
                     QPEQFVEISETLAAAKGIANGDRVTVSSKRGFIRAVAVVTRRLKPLNVNGQQVETVGI
                     PIHWGFEGVARKGYIANTLTPNVGDANSQTPEYKAFLVNIEKA"
     gene            4867..5748
                     /gene="yddG"
                     /locus_tag="ACHKBL_002753"
     CDS             4867..5748
                     /gene="yddG"
                     /locus_tag="ACHKBL_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415990.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid efflux DMT transporter YddG"
                     /protein_id="HFO5819605.1"
                     /translation="MTRQKATLIGLIAIVLWSTMVGLIRGVSEGLGPVGGAAAIYSLS
                     GLLLIFTVGFPRIRQIPKGYLLAGSLLFVSYEICLALSLGYAATRHQAIEVGMVNYLW
                     PSLTILFAILFNGQKTNWLIVPGLLLALVGVCWVLGGDNGLHYDEIINNITTSPLSYF
                     LSFIGAFIWAAYCTVTNKYARGFNGITVFVLLTGASLWVYYFLTPQPEMVFSTPVMIK
                     LISAAFTLGFAYAAWNVGILHGNVTIMAVGSYFTPVLSSALAAVLLSAPLSFSFWQGA
                     LMVCGGSLLCWLATRRG"
     gene            6021..7409
                     /gene="narU"
                     /locus_tag="ACHKBL_002754"
     CDS             6021..7409
                     /gene="narU"
                     /locus_tag="ACHKBL_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter NarU"
                     /protein_id="HFO5819606.1"
                     /translation="MALQNEKNSRYLLRDWKPENPAFWENKGKHIARRNLWISVSCLL
                     LAFCVWMLFSAVTVNLNKIGFNFTTDQLFLLTALPSVSGALLRVPYSFMVPIFGGRRW
                     TVFSTAILIIPCVWLGIAVQNPNTPFGIFIVIALLCGFAGANFASSMGNISFFFPKAK
                     QGSALGINGGLGNLGVSVMQLVAPLVIFVPVFAFLGVNGVPQADGSVMSLANAAWIWV
                     PLLAIATIAAWSGMNDIASSRASIADQLPVLQRLHLWLLSLLYLATFGSFIGFSAGFA
                     MLAKTQFPDVNILRLAFFGPFIGAIARSVGGAISDKFGGVRVTLINFIFMAIFSALLF
                     LTLPGTGSGNFIAFYAVFMGLFLTAGLGSGSTFQMIAVIFRQITIYRVKMKGGSDEQA
                     QREAITETAAALGFISAIGAVGGFFIPQAFGMSLNMTGSPVGAMKVFLIFYIVCVLLT
                     WLVYGRRKFSQK"
     gene            7504..11244
                     /gene="narZ"
                     /locus_tag="ACHKBL_002755"
     CDS             7504..11244
                     /gene="narZ"
                     /locus_tag="ACHKBL_002755"
                     /EC_number="1.7.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase Z subunit alpha"
                     /protein_id="HFO5819607.1"
                     /translation="MSKLLDRFRYFKQKGETFADGHGQVMHSNRDWEDSYRQRWQFDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWEIQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPLIRKRLIELWREALKQHSDPVLAWASIMNDPQKCLSYKQVRGRGGFIR
                     SNWQELNQLIAAANVWTIKTYGPDRVAGFSPIPAMSMVSYAAGTRYLSLLGGTCLSFY
                     DWYCDLPPASPMTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTIAITPDYSEVAKLCDQWLAPKQGTDSALAMAMGHVILKEFHLDNPSDYFINYCRRY
                     SDMPMLVMLEPRDDGSYVPGRMVRASDLVDGLGESNNPQWKTVAVNTAGELVVPNGSI
                     GFRWGEKGKWNLESIAAGTETELLLTLLGQHDAVAGVAFPYFGGIENPHFRSVKHNPV
                     LVRQLPVKNLTLADGNTCPVVSVYDLVLANYGLDRGLEDENSAKDYAEIKPYTPAWGE
                     QITGVPRQYIETIAREFADTAHKTHGRSMIILGAGVNHWYHMDMNYRGMINMLIFCGC
                     VGQSGGGWAHYVGQEKLRPQTGWLPLAFALDWNRPPRQMNSTSFFYNHSSQWRYEKVF
                     AQELLSPLADASKYSGHLIDFNVRAERMGWLPSAPQLGRNPLGIKAEADKAGLSPTEF
                     TAQALKSGDLRMACEQPDSGSNHPRNLFVWRSNLLGSSGKGHEYMQKYLLGTESGIQG
                     EELGPTEGIQPEEVEWQTAAIEGKLDLLVTLDFRMSSTCLFSDIVLPTATWYEKDDMN
                     TSDMHPFIHPLSAAVDPAWESRSDWEIYKGIAKAFSQVCVGHLGKETDMVLQPLLHDS
                     PAELSQPCEVLDWRKGECDLIPGKTAPNIVAVERDYPATYERFTSLGPLMDKLGNGGK
                     GISWNTQDEIDFLGKLNYTKRDGPAQGRPLIDTAIDASEVILALAPETNGHVAVKAWQ
                     ALGEITGREHTHLAQHKEDEKIRFRDIQAQPRKIISSPTWSGLESDHVSYNAGYTNVH
                     ELIPWRTLSGRQQLYQDHPWMRAFGESLVAYRPPIDTRSVSEMRQIPPNGFPEKALNF
                     LTPHQKWGIHSTYSENLLMLTLSRGGPIVWISETDARELTIVDNDWVEVFNANGALTA
                     RAVVSQRVPPGMTMMYHAQERIMNIPGSEVTGMRGGIHNSVTRVCPKPTHMIGGYAQL
                     AWGFNYYGTVGSNRDEFIMIRKMKNVNWLDDEGRDQVQEAKK"
     gene            11241..12785
                     /gene="narH"
                     /locus_tag="ACHKBL_002756"
     CDS             11241..12785
                     /gene="narH"
                     /locus_tag="ACHKBL_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HFO5819608.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTGREGMEYAWFNNV
                     ETKPGIGYPKNWEDQEEWQGGWVRDVNGKIRPRLGSKMGVITKIFANPVVPQIDDYYE
                     PFTFDYEHLHSAPEGKHIPTARPRSLIDGKRMDKVIWGPNWEELLGGEFEKRARDRNF
                     EAMQKEMYGQFENTFMMYLPRLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCRG
                     WRLCISGCPYKKIYFNWKSGKSEKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYDA
                     DRIEEAASTEREVDLYERQCEVFLDPHDPSVIEEALKQGIPQNVIEAAQRSPVYKMAM
                     DWKLALPLHPEYRTLPMVWYVPPLSPIQSYADAGGLPKSEGVLPAIESLRIPVQYLAN
                     MLSAGDTGPVLRALKRMMAMRHYMRSQTVEGVTDTRAIDEVGLNVAQVEEMYRYLAIA
                     NYEDRFVIPTSHREMAGDAFAERNGCGFTFGDGCHGSDSKFNLFNSSRIDAINITEVR
                     DKAEGE"
     gene            12785..13480
                     /gene="narW"
                     /locus_tag="ACHKBL_002757"
     CDS             12785..13480
                     /gene="narW"
                     /locus_tag="ACHKBL_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HFO5819609.1"
                     /translation="MQILKVIGLLMEYPDELLWECKEDALALIRRDAPMLTDFALDLL
                     NAPLLDKQAEWCEVFDRGRTTSLLLFEHVHAESRDRGQAMVDLLAEYEKVGLQLNCRE
                     LPDYLPLYLEYLSILPDDQAKEGLLNVAPILALLGGRLKQREAPWYALFDALLQLAGS
                     PLSSDSVTKQIRSEERDDTRQALDAVWEEEQVKFIEDNATACDSSPLNQYQRRFSQDV
                     APQYVDISAGGGK"
     gene            13477..14157
                     /gene="narI"
                     /locus_tag="ACHKBL_002758"
     CDS             13477..14157
                     /gene="narI"
                     /locus_tag="ACHKBL_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HFO5819610.1"
                     /translation="MIQYLNVFFYDIYPYLCGTVFILGSWLRYDYGQYTWRAASSQML
                     DKRGMVLWSNLFHIGILGIFFGHLFGMLTPHWMYAWFLPVAVKQQMAMILGGICGVLT
                     LIGGAGLLVRRLTNPRIRATSSGADILILAILLIQCILGLTTIPFSAQHPDGSEMMKL
                     VGWAQSIVTFQGGASTHLDGVAFIFRVHLVLGMTIFLLFPFTRLVHVWSAPFEYFTRR
                     YQIVRSRR"
     gene            14237..15130
                     /locus_tag="ACHKBL_002759"
     CDS             14237..15130
                     /locus_tag="ACHKBL_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family isomerase"
                     /protein_id="HFO5819611.1"
                     /translation="MKPQVYHVDAFTSQPFRGNSAGVVFPADNLSEAQMQLIARELGH
                     SETAFLLHSDDSDVRIRYFTPTVEVPICGHATVAAHYVRAKVLGLGNCTVWQTSLAGK
                     HRVTIEKHNDDYRISLEQGTPGFESPLEGETRAAIINALHLTEDDILPGLPIQVATTG
                     HSKVMIPLKPEVDIDALSPDLNALTAISKQIGCNGFFPFQIRPDKNETDGRMFSPAIG
                     IVEDPVTGNANGPMGAWLVHHNVLPHDGNVLRVKGHQGRALGRDGVIEVTVTIRDNQP
                     EKVTISGVAVILFHAEWAIEL"
     gene            complement(15226..16071)
                     /gene="nhoA"
                     /locus_tag="ACHKBL_002760"
     CDS             complement(15226..16071)
                     /gene="nhoA"
                     /locus_tag="ACHKBL_002760"
                     /EC_number="2.3.1.118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-hydroxyarylamine O-acetyltransferase"
                     /protein_id="HFO5819612.1"
                     /translation="MTPILNHYFARINWSGAAAANIDTLRALHLKHNCTIPFENLDVL
                     LPREIQLDDQSLEEKLVIARRGGYCFEQNGVFERVLRELGFNVRSLLGRVVLSNPPAL
                     PPRTHRLLLVELEGEKWIADVGFGGQTLTAPIRLMPDIMQTTPHGEYRLLQEGDDWVL
                     QFNHHQHWQSMYRFDLCEQQQSDYVMGNFWSAHWPQSHFRHHLLMCRHLPDGGKLTLT
                     NFHFTHYENGHAVEQRNLPDVASLYAVMQEQFGLGVDDAKHGFTVDELALVMAAFDTH
                     PEAGK"
     gene            16246..16815
                     /locus_tag="ACHKBL_002761"
     CDS             16246..16815
                     /locus_tag="ACHKBL_002761"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HFO5819613.1"
                     /translation="MSQFIPVELHHASRLLNHGPTVLITSFDEQSQRRNIMAAAWSMP
                     VEFEPPRVAIVVDKSTWTRELIERNGKFGIVIPGVAATNWTWAVGSVSGREEDKFYCY
                     GIPVVKGPVLGLPLVEEKCLAWMECRLLPATSAQEKYDTLFGEVVSAAADARVFVEGR
                     WQFDDDKLNTLHHLGAGTFVTSGKRVTAG"
     gene            complement(16969..17349)
                     /locus_tag="ACHKBL_002762"
     CDS             complement(16969..17349)
                     /locus_tag="ACHKBL_002762"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO5819614.1"
                     /translation="MIIRPVKPSDVEPLLDMLLASGQFNQDGLHHVQKTLTHYFSGQS
                     ADLWFSAEHLGLAGIAYCAPEVMTNDVWNLLMHWISPSHQRQGVGHLLINEIEKELTI
                     KQARLLLVETSSYVDFSAARAFYT"
BASE COUNT         4173 a         4653 c         4503 g         4360 t
ORIGIN      
        1 ggcgcttctt aaagcgccat tttcagctta aagttcaaaa gagattatat cccttcttca
       61 ctctcttttt tcgcttccgc cttctcgatt tcacgatacc agcgcggatg gtgtttcttc
      121 gcccagcgac ggctcacctt cccttcgatc atccctttaa tcgatccttt cacccagaat
      181 gccatataca tatggatcag gatggcgtgg atcaggatga tacccgcagc cgcgtgaatc
      241 agcaggctgt agcgaacaac ctgcatcggg aagtactgcg caaagtacgg acgccagata
      301 atcaccccgg tcaccagcag cacgaaaatc atgctcatga tcgaccagaa catcatcttc
      361 tgcccggcgt tgtacttacc aacatccgcc actttgtgct cattgccttt taatacttcg
      421 acaatgttca acagccacgg aatatccttc ttatccggga tattgtgatg tacaaaacgc
      481 acaaacataa acatcagcgc gacgaaaatc gcaatgccga agaacgggtg caaaatgcgt
      541 cccatctgcg gcgtaccgaa ggtttgcgtc agccattgca gcgtcgggaa gaagaacgaa
      601 atcccggaca gcgccaccag gaagaagcaa atcaccacgg tccagtgaca ggcgcgatca
      661 ataaacttgg tgcgcacaat cattttcgac ttactcatga tgctcctcct cgtcatcgtc
      721 cacttcctta ttcgggccaa taccgatgta gtggaaaatc aacccggcaa aagtggcgat
      781 aaagccagcc gctgccagcg gtttcaacgc gcctttccac aagttcaccg atgtgtcgat
      841 cttcggatct ttcggcagac cgtgatacag ctccggctga tcggcgtgat gcaacacgta
      901 catgacgtgc gtaccaccga ccccttccgg gttgtagacg ccagcatgtt cgtaaccacg
      961 cgctttcagt ttcgctacgc gctgttccgc cagctccagc atctccttct tggtgccgaa
     1021 gtggatagcc ccggtcggac aggttttcac acaagccggt tcctggccga cgctgacgcg
     1081 atcgacgcag agcgtgcatt tatatacccg gttatcctct ttgttgaggc gcggaatatt
     1141 aaacggacac ccggcaatgc agtaaccaca gccgatgcag ttttccgact ggaaatcaac
     1201 aatcccgtta gcgtactgaa tgattgcacc agcagacggg cacgccttca ggcagcccgg
     1261 atcttcacag tgcatacagc catctttacg gatcagccac tccagcttgc cgttctgttc
     1321 ggtttcgcta aagcgcatca ccgtccagga cttggcgctc agatcggcgg ggttatcgta
     1381 aaccccgacg cagtgcccca cttcatcacg gatgtcgttc cactccgaac acgccacctg
     1441 acaggcttta cagccgatac aggtggaaac gtcgataagt ttcgcgactt ctgctttgta
     1501 atcacgcacc tgagaaggcg gcgtgatgga gttagttgcg gaccttttga taatgtcctg
     1561 cgtttccata gccatctgtt cgccccctta cgccttctcg atgtttacta agaacgcttt
     1621 atattccggc gtttgcgagt ttgcatcacc gacattcggc gtcagagtgt tagcgatata
     1681 acctttacgc gcgacaccct caaagcccca gtggattgga atacccaccg tttcaacctg
     1741 ctgaccgttt acattcagcg gtttcagacg gcgcgttacc acagccaccg cgcggataaa
     1801 gccacgcttg ctggagacag tgacacgatc gccattggca atgcctttcg ccgccgccag
     1861 cgtttcgctg atttccacaa actgttccgg ctgagcaatt gcgttgagca atgcgtgctt
     1921 ggtccaggtg tggaagtgct ccgtcagacg ataggtcgta cccacatacg ggaactgctc
     1981 ttttttaccc atccgcagtg cgtcttgttc atacagacga acaaccgggt tagacaccac
     2041 gttcggatgc aacgggttag tgcccagcgg cgtttcaatt ggctcatagt gttccgggaa
     2101 cggaccttcc gccattttgt tgatggcaaa caggcgtccc atcccttccg gctgcatgat
     2161 aaacggcccg gttggcgtac ccggtgcggc attgccgaag tccggaatat cgttacccgt
     2221 ccacttgcta ccgttccact ggatcagcat ccgtttcgga tcccacggtt taccgttgat
     2281 atccgccgaa gcacggttgt agagtacgcg acggttgagc ggccacgccc aggcccatcc
     2341 cagcgtattc cccagaccgg acgggtcgga gttatcgcgg ttagccatct ggttgccctg
     2401 ctcggtccag ctaccggtgt agatccagca agaagatgcg gttgtaccgt catcacgcag
     2461 atgcgcaaag ctactcagca actgaccttt cttcgcaatc agcacgccat tagcgtcata
     2521 gagatcttcc agcgcatagc cgttgttctc tttcgccact tcgtcagact gcggttcgtg
     2581 cggctgcttg tagttccagc tcatcttcat cagcggttct acgcctttac caccctcggc
     2641 ctggtacagc tcgcgcagat gatggtagat acccgccaga atttcgccgt cgttacgtgc
     2701 ttcgcccggc gcgtcctgac ctttccagtg ccactgcaac cagcgaccgg agttagcgat
     2761 agaaccatct tcttcagcaa agcaggtcga aggcagacgg aatacttcag tctgaataga
     2821 tgccggatcg acatcgttcg actcgccgtg gttctgccag aaggtagagg tttcagtcac
     2881 cagcggatcg ataaccacca tgtacttcag tttgctcagg cagctcacca ctttgttttt
     2941 gtccgggaag gacgcaaccg ggttaaagcc ctggcagaaa taaccggtga ctttaccttc
     3001 atccatcatg ttgaaatact tgatgacgtc gtaggtctgg tcccacttcg gcagccagtc
     3061 gtagccccag ttgttctctt tctgcgcggc atcgccgtag aaagatttca tcaggctaac
     3121 gaaaaacttc ggatagttgc tccagtagtt cacctgatca gccagcgtcg ctttcggcgt
     3181 gttcgcttcc agatacgact gcaaatcaac ctgtttttct gacggcagcg tcagataacc
     3241 tggcaggctg gtagagagca ggcctaagtc agtcaggccc tgaatgttgg agtgaccacg
     3301 caatgcgttc acgccgccac cggccatacc catgttaccg agcagcaact ggatcatcgc
     3361 catcgtacgg atgttctgcg cacccacggt gtgctgcgtc cagcccagcg cgtacaggaa
     3421 ggtggttgtg cgatccggtg cgctggtgga ggccagcact tcacacactt tcaggaagtc
     3481 ggcttttggc gtaccacaga tgttttcaac aacgtccggc gtgtagcggg aaacgtgcgc
     3541 tttcagcagg ttccacacac agcgcggatg agtcagtgtt tcatcgcgtt tcgcatagcc
     3601 gttttcatcg aactgatagt tccaggacga tttatcgtat tgccgttttt cagcgtcgta
     3661 gccgctgaac agaccgtctt cgaaagcaaa atcatcacgc accagcaggc tggcattggt
     3721 gtaatgctta acgtattcgg cattgatttt gttgttttcg atcaggtagc gcaaaacgcc
     3781 agacaggaac gtaatgtccg taccggaacg aataggtgcg taaatatccg ctacagaagc
     3841 ggtacgcgta aaacggggat cgacaacgat caaggttgca tcgttgttgt ttttcgcttc
     3901 catcgcccag cggaaaccga cgggatgcgc ttcagcagcg ttaccgccca tcaccattac
     3961 gacgttagcg tttttgatat ccacccagtg gttggtcatc gcaccgcgac caaatgttgg
     4021 agcaagactt gctaccgttg gtccgtgtca gacgcgcgcc tggttgtcta ctgccagcat
     4081 cccgagggag cgggcaaatt tttgagtcag catcccggtt tcgttgctgg caccggaggc
     4141 acacagcata ccggtagaaa gccaacggtt taccgttacg ccctgctcgt tcttttcaat
     4201 aaagttagcg tcacggtcag ctttcatcag cttcgcaata cgggagaatg cttcttccca
     4261 gctaatgcgc tgccatttgt cagaacctgg cgcacgatat tccgggtagc gcagacggtt
     4321 ttcgctgttg acgtaatcca gcaaaccggc ccctttcggg cacagcgcac cacggcttac
     4381 cggatgatcc gggtcacctt caatgtgata aatcgcttct ctggcgtttt tcgcgccatc
     4441 acccaggcta tacatcaata gcccgcaacc tacggaacag tatgtgcagg tgttacggat
     4501 ctctttcgcg cgtaataatt tgtagtttcg cgcctgagcc agtgcttgct tcggggcaaa
     4561 gcccaatgcc gctaccgttg ttccagccat accgcccgcg cagattttaa aaaattgtct
     4621 gcgactgacg tccattgctt tcctcttttt tcaggggtat tactgcgcga ggaaactaac
     4681 agtaaaattc tgaatctttt tgcgcgaaat caaaaagaac acgtgtttgc cacaaaataa
     4741 tccgcctgcg caaattgacc tacctcaata gcggtagaaa aacgcaccac tgcctgacag
     4801 gccagttaaa aaaatgctat aaaattcagc ttaattttta acggcaagag agacaaaaca
     4861 gcgagcatga cacgacaaaa agcaacgctc atagggctaa tagcgatcgt cctgtggagc
     4921 acgatggtag gattgattcg cggtgtcagt gaggggctcg gcccggtcgg cggcgcagct
     4981 gctatctatt cattaagcgg gctgctgtta atcttcacgg ttggatttcc gcgtattcgg
     5041 cagatcccga aaggctattt actcgccggg agtctgttat tcgtcagcta tgaaatctgt
     5101 ctggcgcttt ccttagggta tgcggcgacc cgtcatcagg cgattgaagt ggggatggtg
     5161 aactatctat ggcccagcct gacaattctc tttgccattc tgtttaatgg tcagaaaacc
     5221 aactggctga ttgtaccagg attattatta gccctggtcg gcgtctgctg ggtgttaggt
     5281 ggtgacaatg ggttacatta tgatgaaatc atcaataata tcaccaccag cccattgagt
     5341 tatttcctgt cgtttattgg tgcgtttatc tgggcagcct actgcacagt aacgaataaa
     5401 tacgcacgcg gatttaacgg aattaccgtt tttgtcctgc taacgggagc aagtctgtgg
     5461 gtttactatt ttctcacgcc acaaccagaa atggtattta gcacgccagt catgattaaa
     5521 ctcatctctg cggcatttac cttaggattt gcttatgctg catggaacgt cggtatatta
     5581 catggcaacg tcaccattat ggcggtaggt tcgtatttta cgcccgtcct ttcctcagcg
     5641 cttgcagccg tgctgctcag cgccccgctg tcgttctcgt tctggcaagg cgcgctaatg
     5701 gtctgcggcg gttccctgct ctgctggctg gcgacacgtc gtggctaaaa taattgtcgg
     5761 gtctacggcc cggcagttaa tcagataaat tctgacaata ttaaaaaata aatttcaaat
     5821 accagaaagc cattttagtt acatttagta acacatatct gtctgtttaa tccacattct
     5881 gcccccgcag ttacgctttc ttatctattc ttctaatcag cacttcacct gattcatcct
     5941 taattctaat aactggctat tgcgccttac ctcctgtttt gtcttttttt cattaccaat
     6001 gtgtgcatgt gaggaacaat atggcactgc aaaatgagaa aaatagtcgt tatcttttac
     6061 gcgactggaa accagaaaat ccggccttct gggaaaataa aggaaagcat attgcacgaa
     6121 gaaacctctg gatatcagtc agttgcctac ttcttgcctt ctgtgtctgg atgctattta
     6181 gcgcagttac tgttaatctc aataaaatcg gttttaattt cactaccgat caactctttt
     6241 tattaaccgc attaccctcc gtttctggcg cattattgcg tgtcccctac tcctttatgg
     6301 tgcctatatt cggtggacgc cgatggacgg tttttagtac tgcaatcctg attattcctt
     6361 gcgtctggct cggaattgcc gtgcagaatc cgaatactcc ttttgggata tttatcgtta
     6421 tcgctttgct atgcggtttt gcaggtgcga actttgcttc gagcatgggc aatatcagtt
     6481 tcttctttcc aaaagccaaa caagggagcg ctcttgggat taatggcgga ttaggaaact
     6541 taggtgtaag tgtgatgcag ctggttgcac cgctggtcat ttttgtcccc gtatttgcct
     6601 ttctcggcgt caatggcgta ccgcaggccg acggttcggt gatgtcgctg gcgaatgccg
     6661 catggatttg ggtgccatta ctggcgattg ccacgatcgc cgcctggtca gggatgaatg
     6721 atatcgccag ttcacgcgcc tcaattgccg accagctccc tgtcttacaa cgcctgcatc
     6781 tctggctgct gagcctgctt taccttgcca ccttcggttc gtttatcggt ttttctgcgg
     6841 gttttgccat gctggcaaaa acccagttcc cggatgtgaa tattctgcgc ctggcgttct
     6901 ttggcccatt tatcggtgcc atcgcgcgat cggttggtgg tgctatttcc gataaattcg
     6961 gcggcgtgcg ggtgacgttg atcaacttca tttttatggc gattttcagc gccctgctgt
     7021 tccttacctt accgggcaca ggctccggta atttcatcgc attttacgcc gtatttatgg
     7081 ggctgtttct gaccgcgggt ctgggaagtg gttctacttt ccagatgatc gccgtcatct
     7141 ttcgccagat aacaatttat cgggtgaaga tgaaaggcgg tagtgatgag caagctcaaa
     7201 gagaagccat caccgaaacg gcagccgctc tgggctttat ctcagccatt ggcgcagtgg
     7261 gcggcttttt tattccgcag gcgtttggca tgtcgctcaa tatgaccggc tctccggtgg
     7321 gcgcgatgaa agtgttttta atcttctaca tcgtttgtgt gctactgacc tggctggttt
     7381 atggtcggcg gaagttcagc caaaaataag agatcgatgc ctgacacgtc ttatcaggct
     7441 acaaccgtgt cgcatccgac aaggcatcag atcccaattc attcctggag caggagttat
     7501 gtcatgagta aacttttgga tcgctttcgc tacttcaaac aaaagggcga aacctttgcc
     7561 gatggtcacg gacaggtgat gcatagcaac cgcgactggg aggacagcta tcgccagcgt
     7621 tggcagttcg acaaaatcgt gcgttccacc cacggtgtta actgtacagg ctcctgtagc
     7681 tggaaaatct acgttaaaaa tggtctggtg acctgggaaa tccaacagac cgactacccg
     7741 cgcactcgcc ctgacctgcc aaatcatgaa cctcgcggct gcccgcgtgg cgcaagttac
     7801 tcctggtatc tctacagcgc taaccgcctg aaataccctc tcattcgtaa acgactgatt
     7861 gaactgtggc gcgaagccct caagcaacac agcgatccgg tactggcgtg ggcatcgatt
     7921 atgaacgatc cgcaaaagtg cctgagctac aaacaagtac gtggacgcgg cggctttatc
     7981 cgctctaact ggcaggagct aaatcagctg attgctgccg ctaacgtctg gaccatcaaa
     8041 acctacggcc cggatcgcgt tgccggtttc tcgccgatcc cggcgatgtc gatggtttct
     8101 tacgccgccg gaacacgtta tctttcacta ttaggtggca cctgtttaag cttctacgac
     8161 tggtattgcg acctgccgcc cgcctcgccg atgacctggg gcgagcaaac cgacgtaccg
     8221 gaatctgccg actggtataa ctccagttac atcatcgcct gggggtctaa cgtaccgcag
     8281 acacgtacgc cggacgccca tttctttacc gaagtacgct acaaaggcac taaaaccatc
     8341 gccattaccc ctgactactc tgaagtggcc aaattgtgcg accagtggct ggcaccgaaa
     8401 caaggcactg atagtgccct ggcaatggca atgggccatg tcattttaaa agagtttcat
     8461 ctcgataatc ccagcgatta ttttattaac tactgccgcc gctacagcga catgccgatg
     8521 ctggtaatgc tggaacctcg cgacgacggc agctacgttc ccgggcgcat ggtccgcgca
     8581 tctgacctgg tggatggact gggcgaaagc aataatccgc agtggaaaac cgtggcagtt
     8641 aataccgcag gtgaactggt agtgccgaac ggttcgattg gttttcgctg gggagaaaaa
     8701 ggcaaatgga atctggaatc cattgccgcc ggtacggaaa ccgaattgtt gttaactctg
     8761 ctcggtcaac atgatgccgt tgcaggcgtg gccttcccct actttggcgg cattgaaaat
     8821 ccgcattttc gcagcgtaaa acacaatccg gtcctggtgc gccaattgcc cgttaaaaac
     8881 ctgacattag ccgatggcaa cacctgtccg gtcgtcagcg tttatgattt ggtactggcg
     8941 aattatggcc tcgatcgcgg gctggaagat gaaaacagcg cgaaagatta cgctgaaatc
     9001 aaaccgtaca ccccagcctg gggtgagcaa attaccggcg tgccgcgcca gtatattgaa
     9061 accatcgccc gtgaatttgc cgataccgcc cataaaacgc atgggcgctc gatgattatc
     9121 ctcggtgctg gcgtcaacca ttggtatcac atggacatga actaccgtgg gatgatcaac
     9181 atgctgatct tctgcggttg tgtcgggcaa agcggtggcg gctgggcaca ctatgtcggt
     9241 caggaaaaac tgcgcccgca aaccggctgg ttgccgctgg cctttgcgct cgactggaac
     9301 cgaccaccgc gccagatgaa cagcacctcg tttttctaca atcattccag ccaatggcgc
     9361 tatgaaaaag tctttgctca ggagttactt tcaccgctcg ccgatgccag taagtacagc
     9421 ggtcatctga ttgatttcaa cgttcgtgcc gaacgtatgg gctggctacc ttctgcgccg
     9481 cagctggggc gtaacccgct cgggattaaa gctgaagccg acaaggccgg attatccccc
     9541 acagaattta ccgcccaggc gctgaaatcg ggcgatttac gcatggcctg cgaacaacca
     9601 gatagcggca gcaatcatcc gcgtaatttg tttgtctggc gttctaacct gcttggctcc
     9661 tccggcaaag gccacgagta tatgcagaag tatctgctgg gtacggaaag tggcattcag
     9721 ggggaagaac ttggaccgac ggagggaatc caaccggaag aagttgagtg gcaaactgca
     9781 gcgattgaag gcaagctcga cctgctggtg acgcttgact tccgcatgtc cagcacctgc
     9841 ctgttctccg atatcgttct gcccaccgcc acctggtacg aaaaagacga tatgaatacc
     9901 tcggatatgc atccgtttat tcatccgctt tctgcggcgg tcgatccggc gtgggaatca
     9961 cgcagcgact gggaaatcta caaaggtatc gctaaagcct tttcgcaagt gtgcgtgggg
    10021 catctcggca aagaaaccga catggtatta caaccactgc tgcatgactc cccggcagag
    10081 ctttcacagc cgtgtgaagt gctcgactgg cgcaaaggtg aatgcgatct gattccaggc
    10141 aaaaccgcgc cgaatattgt ggcggtggag cgcgactacc ctgctaccta tgaacgcttt
    10201 acctcactcg ggccattgat ggacaaactt ggcaacggcg gtaaagggat ttcgtggaat
    10261 acccaggatg aaatcgattt cctcggtaaa ctcaattaca ccaagcgtga tggcccggca
    10321 caggggcgtc cgctgattga taccgcgatt gacgcatcag aagtgattct ggcgctggca
    10381 ccggaaacca acggccatgt tgcagtcaaa gcgtggcaag cgctgggcga gatcaccgga
    10441 cgcgaacata cccatctggc gcagcacaaa gaggacgaga agattcgctt tcgcgatatt
    10501 caggcgcagc cgcgtaaaat tatctccagc cccacatggt ctggtctgga aagcgatcac
    10561 gtttcctata acgcgggata caccaacgtt catgagttaa ttccgtggcg cacgctgtcg
    10621 ggacgccagc aactctatca ggatcatccg tggatgcgtg cttttggtga aagtctggtg
    10681 gcttatcgcc cgcctatcga cacccgcagt gtcagtgaaa tgcgccagat accgccaaac
    10741 ggcttcccgg agaaagccct taacttcctg acaccgcacc agaaatgggg cattcactca
    10801 acctacagtg aaaacctgct aatgctgacg ctctctcgcg gtggaccgat tgtctggatc
    10861 agcgaaaccg atgcccgtga actgaccatt gtcgataacg actgggtgga agtgtttaac
    10921 gccaatggcg cgctgacggc ccgcgcggtg gtcagccagc gtgtaccacc gggcatgacc
    10981 atgatgtatc acgctcagga acgcattatg aatattccag gttcggaagt aaccggcatg
    11041 cgcggcggca ttcataactc ggtcacccgc gtttgtccga aaccaacgca tatgattggc
    11101 ggttacgcgc agctggcctg gggctttaat tactacggca ccgtcggctc gaaccgtgac
    11161 gaattcatca tgatccgcaa gatgaagaac gttaactggc tggatgatga aggtcgcgat
    11221 caggtacagg aggcgaaaaa atgaaaatac gttcgcaagt cggcatggtg cttaacctcg
    11281 ataaatgtat cggctgccat acctgttcgg tgacctgtaa aaacgtctgg accggacgcg
    11341 aaggcatgga gtacgcatgg tttaacaacg tcgaaaccaa accgggcatt ggttatccga
    11401 aaaactggga agatcaggaa gaatggcaag gcggctgggt gcgtgatgtg aatggcaaga
    11461 tccgcccgcg tctgggcagc aagatgggcg taataaccaa aatcttcgcc aacccggtgg
    11521 tgccgcagat tgatgattac tacgaacctt tcaccttcga ctacgaacat ttgcatagcg
    11581 caccggaagg caaacatatc cctactgctc gcccgcgttc actgattgac ggcaaacgga
    11641 tggacaaagt gatctggggg ccaaactggg aagaactgct gggcggcgaa tttgaaaaac
    11701 gcgctcgcga ccgcaacttc gaggccatgc aaaaggagat gtacgggcag tttgaaaaca
    11761 ccttcatgat gtacctgccg cgcctgtgcg aacactgcct caatcccagc tgcgtggcga
    11821 cctgcccaag cggtgctatc tacaaacgtg aagaagacgg cattgtgctg attgatcagg
    11881 ataaatgccg tggctggcgt ttgtgcatta gcggttgtcc gtacaaaaaa atctacttca
    11941 actggaaaag cggcaagtca gaaaaatgta tcttctgtta cccacgaatt gagtcaggcc
    12001 aacccaccgt gtgctcagaa acctgcgtgg gtcgcatccg gtatctgggc gtgctgcttt
    12061 acgacgccga ccgcatcgag gaagcggcga gcaccgagcg cgaagttgac ctctatgaac
    12121 gtcagtgcga agtgttcctc gatccgcacg atccctcagt gatcgaagaa gccctgaaac
    12181 aaggtattcc acaaaacgtg attgaagctg cccagcgttc gccagtctac aaaatggcga
    12241 tggactggaa actggcgtta ccgctgcacc ctgaataccg caccctgccg atggtctggt
    12301 acgttcctcc gctgtcaccg attcagtctt acgctgatgc gggcggtttg ccgaaaagcg
    12361 aaggcgtgct gcctgctatc gaaagcctgc gtattccggt gcaatatctc gccaatatgt
    12421 tgagtgctgg cgataccggt ccggtactgc gggcgctgaa acggatgatg gcgatgcgcc
    12481 actatatgcg ttcacaaacc gtggaaggcg ttaccgatac tcgtgccatc gacgaagtag
    12541 gcctgaacgt cgcccaggtc gaagagatgt atcgctacct cgccattgcc aactatgaag
    12601 accgttttgt cattccaacc agtcatcggg aaatggcggg cgatgccttt gctgaacgca
    12661 acggctgcgg ttttaccttt ggcgacggtt gccacggctc ggacagcaaa ttcaacctgt
    12721 tcaacagtag ccgtatcgat gccatcaaca tcaccgaagt gcgcgacaaa gcggagggcg
    12781 aataatgcag atcctcaaag tgatcggcct gttgatggag tatccggacg agctgttgtg
    12841 ggaatgtaag gaggacgcgc tggcgttgat ccgccgcgac gcaccgatgc tcaccgattt
    12901 tgcactcgac ctgctcaacg cgccgctgct ggataaacag gccgaatggt gtgaagtgtt
    12961 tgaccgcggg cgcaccacgt cgctgctgct gtttgaacat gttcatgccg agtcccgcga
    13021 tcgcggccag gcaatggtgg acctgctggc ggagtatgaa aaggtcggtc tgcaactgaa
    13081 ttgtagggaa ctgcccgatt atctaccgct gtatctggag tatttaagca tacttccgga
    13141 cgatcaggcg aaagaaggat tactcaacgt tgcgccgatc ctcgccctgc ttggcggacg
    13201 cttaaaacaa cgcgaggcac cgtggtacgc gttatttgac gccctgttgc aactggcagg
    13261 aagccctcta tcaagtgaca gtgtcacaaa acagattcgc agtgaagagc gtgacgacac
    13321 ccgccaggcg ctggatgcgg tgtgggaaga agaacaggtg aagtttattg aagataacgc
    13381 cacggcgtgt gacagctcgc cgcttaatca atatcagcga cgctttagcc aggatgtcgc
    13441 gccgcagtat gtcgacatca gtgcgggagg tgggaaatga ttcagtatct gaacgtcttt
    13501 ttttacgata tctacccgta cttgtgtggc acggttttta tccttggaag ctggctgcgc
    13561 tacgattacg ggcagtatac ctggcgtgcc gcctccagcc agatgctgga taaacgcggg
    13621 atggtgctat ggtcgaactt gttccatatc ggcattctgg ggatcttctt cggtcatctg
    13681 ttcggcatgt taacgccgca ctggatgtac gcgtggtttc tgccggtggc ggtaaaacag
    13741 cagatggcga tgatcctcgg cggaatatgc ggggtattga cgctgattgg cggtgcgggg
    13801 ctactggtgc gtcgtttaac aaatccgcgt atcagggcga cgtcttctgg tgcggatatc
    13861 ctgatcctcg ccattttgtt aattcagtgc attcttgggt taaccaccat tcctttttca
    13921 gcacaacatc cggacggcag tgaaatgatg aagctggtgg gctgggcgca aagcattgtc
    13981 actttccagg gcggtgcatc tacgcatctt gatggtgtcg cctttatatt ccgcgttcat
    14041 ctggtgcttg gaatgaccat cttcctgctc ttcccgttca cccgactggt gcatgtctgg
    14101 agcgcgccgt ttgagtactt tactcgtcgg tatcagattg tgcgttcgcg gcgttaattc
    14161 tcacgcgggc cggacagccc ttcgctctcc ggccacttgc aggctatgat agttctccag
    14221 ccacaggaga acattaatga aaccgcaggt ttaccacgtc gatgctttta cctctcaacc
    14281 gtttcgcggc aattctgccg gtgttgtttt ccccgccgat aatctcagtg aagcgcaaat
    14341 gcagcttatc gcccgcgagt taggtcattc ggaaactgct tttctgctgc acagcgacga
    14401 cagcgatgtg cgcatccgct actttacgcc aacggttgaa gttccgattt gtggtcacgc
    14461 gacagtagct gcgcactatg tacgtgcaaa ggtgttaggt ttaggaaatt gcacggtctg
    14521 gcaaacatcg ctggcaggaa aacatcgcgt gactatcgaa aagcacaacg atgattatcg
    14581 tatttcgctg gaacaaggta cgccgggctt tgagtcaccg ctggaaggtg aaacacgtgc
    14641 ggcgattatc aacgcgcttc atctcactga agacgatatt ctgccaggct tgccaattca
    14701 ggtggcaacc acaggacact caaaagtgat gatcccactg aaaccggaag tggatatcga
    14761 cgccctttcg cctgacctta atgcgctgac cgctatcagc aaacagattg gctgcaatgg
    14821 tttcttcccg ttccagatcc gcccagacaa aaacgaaacc gacggtcgta tgttctcgcc
    14881 tgccattggt attgtggaag atccggtgac tggcaatgcc aatggcccga tgggtgcatg
    14941 gttagtacat cacaacgtat tgccccacga tggcaacgtg ttgcgtgtta aaggccatca
    15001 ggggcgcgca ttagggcgtg acggcgtgat tgaagtgacg gtgacaattc gtgataacca
    15061 accggagaaa gtcaccattt ctggcgtggc ggtgattttg tttcatgccg aatgggcaat
    15121 tgaactctga aaccgttgcc tgatgcgctt cgcttatcag gtctacaacc gggctaatgt
    15181 aggccggata aggcgtttac gccacatccg gcaacctgac ataaattatt ttcccgcctc
    15241 cgggtgcgta tcaaacgccg ccatcaccag cgctaactca tccacagtaa agccatgttt
    15301 cgcatcatct acgcccagac caaactgttc ctgcatcacg gcatataacg acgccacatc
    15361 tggtagattt cgctgctcaa ctgcgtgccc attttcataa tgggtaaaat gaaaattggt
    15421 cagcgttagc ttgccaccgt ccggcaaatg gcgacacatc agcaaatgat ggcgaaaatg
    15481 cgactgcggc cagtgcgccg accagaaatt gcccatcaca taatcgcttt gttgctgctc
    15541 gcagagatca aaacggtaca tcgactgcca gtgctgatga tgattaaact gcaacaccca
    15601 atcatcaccc tcttgcaaca accgatactc tccgtgtggc gtggtttgca tgatatccgg
    15661 cattaaacga atcggcgcgg ttagcgtctg cccaccgaaa ccgacatcag caatccattt
    15721 ttccccttcc agttccacca acagcaaacg atgggtgcgc ggcggtaatg ctggcggatt
    15781 tgataacact acgcgcccta acaagctgcg aacgttaaac cccagctcgc gtaacacccg
    15841 ctcaaacacg ccattctgct caaaacagta accaccacga cgggctatca ccagtttctc
    15901 ttccagcgat tgatcatcaa gctgtatttc cctcggcagc aaaacgtcga ggttttcaaa
    15961 cggaatggtg caattgtgtt tcaggtgcaa tgcacgaagc gtatcaatat tggccgcagc
    16021 agctcccgac cagttaatac gggcaaaata atgattcaga atgggcgtca tcaggcgttt
    16081 cccttagtgg ttttctcaac ttataacctg gaatagacgc cgcaccggct attttgtgcg
    16141 atcgcggaca tgcatcacgt tcgcaaatcg ccaatcccgt taatttcgtt acttattgcg
    16201 ttacgagcta cgcttttaaa acacccttca tagcgaggcg atatcgtgag ccaattcatt
    16261 cccgtcgaat tacaccatgc cagccgtctg ttaaatcatg gcccaactgt cttgatcacc
    16321 agtttcgatg agcaatccca gcggcgtaac attatggctg cagcctggtc aatgccggtg
    16381 gagtttgaac ctccacgcgt ggcgattgtg gtagataagt cgacatggac cagggagttg
    16441 attgagcgta acggtaaatt tggcatcgtt atcccaggcg ttgcagcgac taactggacg
    16501 tgggcagtgg gaagtgtatc tgggcgtgaa gaggataaat tttattgcta tggcattccg
    16561 gtggtgaaag gtccggtgct tggtttgcca cttgttgaag agaaatgtct ggcgtggatg
    16621 gagtgccgat tgctaccagc gacttccgcg caagagaaat acgacacgct gtttggcgaa
    16681 gtggtatcag cagcggcaga cgcacgggta tttgtcgaag gccgctggca gtttgatgat
    16741 gataagctca atacgttgca tcatttaggc gctggcacgt ttgttaccag cggcaagcga
    16801 gtcaccgcgg gctaaagaat catatgcagc aaccaagttg cttaagcaat gtttcaatat
    16861 gagttaaaag catattttat ctcacgattg catattttta gtgaagatta atttatcctc
    16921 accaacatca tagtaattgc gtattatagc ttcattgata aagccctgtt atgtataaaa
    16981 tgcgcgggcg gcactaaaat cgacatagct ggaggtctct actaaaagta gtcttgcctg
    17041 tttaattgtg agctcctttt ctatttcgtt aatcagcaga tgtcccactc cctggcgctg
    17101 atgggaagga ctgatccagt gcattaataa attccagaca tcatttgtca tcacttcagg
    17161 agcgcaatag gcaatccctg ccagtcctaa gtgttctgca ctaaaccata aatcggcaga
    17221 ttgacctgag aaataatgtg ttagtgtttt ttgtacatga tgaaggccat cttgattaaa
    17281 ttgtccactg gcaagcagca tatccagtaa tggttcaaca tctgaaggtt tgactggtcg
    17341 tataatcatg gaattcctta tctattgatg aatgtttgaa aattgtttgc gtggaacatg
    17401 gttaatctga gccaatattt ttgagaaaga atagatgggt atttatataa gcaaagaagc
    17461 catctcttta aatatcaatt aaagtataac attaacacca gacttcattg cgacaggaca
    17521 cgacaaagca cttttgcggt tttttcaggg tgcaattaca aaaattgaat cccttatgga
    17581 ctaatgtgag aaaattccgg ggcatgacta ttttgatata gtggacaatc aagcgtctcg
    17641 aattaacaat gacttttcag atgctaaacc atgcaattgg aagtatgtc
//
LOCUS       DAZFLD010000098        17663 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.099, whole genome shotgun sequence.
ACCESSION   DAZFLD010000098 DAZFLD010000000
VERSION     DAZFLD010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17663
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.099"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(83..2530)
                     /gene="glgP"
                     /locus_tag="ACHKBL_002763"
     CDS             complement(83..2530)
                     /gene="glgP"
                     /locus_tag="ACHKBL_002763"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HFO5819615.1"
                     /translation="MNAPFTYSSPTLSVEALKHSIAYKLMFTIGKDPVVANKHEWLNA
                     TLFAVRDRLVERWLRSNRAQLSQETRQVYYLSMEFLIGRTLSNAMLSLGIYEDVQGAL
                     EAMGLNLEELIDEENDPGLGNGGLGRLAACFLDSLATLGLPGRGYGIRYDYGMFKQNI
                     VNGSQKESPDYWLEYGNPWEFKRHNTRYKVRFGGRIQQEGKKTRWIETEEILGVAYDQ
                     IIPGYDTDATNTLRLWSAQASSEINLGKFNQGDYFAAVEDKNHSENVSRVLYPDDSTY
                     SGRELRLRQEYFLVSSTIQDILSRHYQLHKTYDNLADKIAIHLNDTHPVLSIPEMMRL
                     LIDEHQFSWDDAFEVCCQVFSYTNHTLMSEALETWPVDMLGKILPRHLQIIFEINDYF
                     LKTLQEQYPNDTDLLGRASIIDESNGRRVRMAWLAVVVSHKVNGVSELHSNLMVQSLF
                     ADFAKIFPGRFTNVTNGVTPRRWLAVANPSLSAVLDEHLGRNWRTDLSLLNDLQQHCD
                     FPMVNHAVHQAKLENKKRLAEYIAQQLNVVVNPKALFDVQIKRIHEYKRQLMNVLHVI
                     TRYNRIKADPDAKWVPRVNIFGGKAASAYYMAKHIIHLINDVAKVINNDPQIGDKLKV
                     VFIPNYSVSLAQLIIPAADLSEQISLAGTEASGTSNMKFALNGALTIGTLDGANVEML
                     DHVGADNIFIFGNTAEEVEELRRQGYKPREYYEKDEELHQVLTQIGSGVFSPEDPGRY
                     RDLVDSLINFGDHYQVLADYRSYVDCQDKVDELYERQEEWTAKAMLNIANMGYFSSDR
                     TIKEYADHIWHIDPVRL"
     gene            complement(2549..3982)
                     /gene="glgA"
                     /locus_tag="ACHKBL_002764"
     CDS             complement(2549..3982)
                     /gene="glgA"
                     /locus_tag="ACHKBL_002764"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HFO5819616.1"
                     /translation="MQVLHVCSEMFPLLKTGGLADVIGALPAAQIADGVDARVLLPAF
                     PDIRRGVTDAQVVSRRDTFAGHITLLFGHYNGVGIYLIDAPHLYDRPGSPYHDTNLFA
                     YTDNVLRFALLGWVGAEMASGLDPFWRPDVVHAHDWHAGLAPAYLAARGRPAKSVFTV
                     HNLAYQGMFYAHHMNDIQLPWSFFNIHGLEFNGQISFLKAGLYYADHITAVSPTYARE
                     ITEPQFAYGMEGLLQQRHREGRLSGVLNGVDEKIWSPETDLLLASRYTRDTLEDKAEN
                     KRQLQIAMGLKVDDKVPLFAVVSRLTSQKGLDLVLEALPGLLEQGGQLALLGAGDPVL
                     QEGFLAAAAEYPGQVGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTL
                     PLVRRTGGLADTVSDCSLENLADGVASGFVFEDSNAWSLLRAIRRAFVLWSRPSLWRF
                     VQRQAMAMDFSWQVAAKSYRELYYRLK"
     gene            complement(3982..5277)
                     /gene="glgC"
                     /locus_tag="ACHKBL_002765"
     CDS             complement(3982..5277)
                     /gene="glgC"
                     /locus_tag="ACHKBL_002765"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HFO5819617.1"
                     /translation="MVSLEKNDHLMLARQLPLKSVALILAGGRGTRLKDLTNKRAKPA
                     VHFGGKFRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVDLL
                     PAQQRMKGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKG
                     ARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPPSMPNDPSKSLASMGIYVF
                     DADYLYELLEEDDRDENSSHDFGKDLIPKITEAGLAYAHPFPLSCVQSDPDAEPYWRD
                     VGTLEAYWKANLDLASVVPELDMYDRNWPIRTYNESLPPAKFVQDRSGSHGMTLNSLV
                     SGGCVISGSVVVQSVLFSRVRVNSFCNIDSAVLLPEVWVGRSCRLRRCVIDRACVIPE
                     GMVIGENAEEDARRFYRSEEGIVLVTREMLRKLGHKQER"
     gene            complement(5295..7268)
                     /gene="glgX"
                     /locus_tag="ACHKBL_002766"
     CDS             complement(5295..7268)
                     /gene="glgX"
                     /locus_tag="ACHKBL_002766"
                     /EC_number="3.2.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HFO5819618.1"
                     /translation="MTQLAIGKPAPLGAHYDGQGVNFTLFSAHAERVELCVFDANGQE
                     HRYDLPGHSGDIWHGYLPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIDG
                     EFKDNPLLHAGHNEPDYRDNAAIAPKCVVVVDHYDWEDDAPPRTPWGSTIIYEAHVKG
                     LTYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNY
                     WGYNPVAMFALHPAYACSPETALDEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPL
                     FSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYASACLRYWVETCHVDGFR
                     FDLAAVMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIAPGGYQVGNFPPLFAEW
                     NDHFRDAARRFWLHYDLPLGAFAGRFAASSDVFKRNGRLPSAAINLVTAHDGFTLRDC
                     VCFNHKHNEANGEENRDGTNNNYSNNHGKEGLGGTLDLVERRRDSIHALLTTLLLSQG
                     TPMLLAGDEHGHSQHGNNNAYCQDNQLTWLDWSQASSGLTAFTAALIHLRKRIPALVE
                     NRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPA
                     GEWHAIPPFAGEDNPVITAVWQGPAHGLCVFQR"
     gene            complement(7265..9451)
                     /gene="glgB"
                     /locus_tag="ACHKBL_002767"
     CDS             complement(7265..9451)
                     /gene="glgB"
                     /locus_tag="ACHKBL_002767"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HFO5819619.1"
                     /translation="MSDRIDRDVINALIAGHFADPFSVLGMHKTTAGLEVRALLPDAT
                     DVWVIEPKTGRKLAKLECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFG
                     PLIQEMDAWLLSEGTHLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWD
                     GRRHPMRLRKESGIWELFIPGAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPETAS
                     LICGLPEKVVRTEERKKANQFDAPISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYA
                     KWMGFTHLELLPINEHPFDGSWGYQPTGLYAPTRRFGTRDDFRYFIDAAHAAGLNVIL
                     DWVPGHFPTDDFALAEFDGTNLYEHSDPREGYHQDWNTLIYNYGRREVSNFLVGNALY
                     WIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNTNRILGEQV
                     SGAVTMAEESTDFPGVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPVYRQYHHDKLT
                     FGMLYNYTENFVLPLSHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLL
                     FMGNEFAQGREWNHDASLDWHLLEGGDNWHHGVQRLVRDLNLTYRHHKAMHELDFDPY
                     GFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFGINQPGKWREILNTD
                     SMHYHGSNVGNGGTVHSDEIASHGRQHSLSLTLPPLATIWLVREAE"
     gene            complement(9724..10827)
                     /gene="asd"
                     /locus_tag="ACHKBL_002768"
     CDS             complement(9724..10827)
                     /gene="asd"
                     /locus_tag="ACHKBL_002768"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFO5819620.1"
                     /translation="MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQAA
                     PSFGGTTGTLQDAFDLEALKALDIIVTCQGGDYTNEIYPKLRESGWQGYWIDAASSLR
                     MKDDAIIILDPVNQDVITDGLNNGIRTFVGGNCTVSLMLMSLGGLFANDLVDWVSVAT
                     YQAASGGGARHMRELLTQMGHLYGHVADELATPSSAILDIERKVTTLTRSGELPVDNF
                     GVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILNTSSVIPVDGLCVRVGALRCHSQ
                     AFTIKLKKDVSIPTVEELLAAHNPWAKVVPNDREITMRELTPAAVTGTLTTPVGRLRK
                     LNMGPEFLSAFTVGDQLLWGAAEPLRRMLRQLA"
     gene            11019..11612
                     /gene="yhgN"
                     /locus_tag="ACHKBL_002769"
     CDS             11019..11612
                     /gene="yhgN"
                     /locus_tag="ACHKBL_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YhgN"
                     /protein_id="HFO5819621.1"
                     /translation="MNEIISAAVLLILIMDPLGNLPIFMSVLKHTEPKRRRAIMVREL
                     LIALLVMLVFLFAGEKILAFLSLRAETVSISGGIILFLIAIKMIFPSASGNSSGLPAG
                     EEPFIVPLAIPLVAGPTILATLMLLSHQYPNQMGHLVIALLLAWGGTFVILLQSSLFL
                     RLLGEKGVNALERLMGLILVMMATQMFLDGIRMWMKG"
     gene            complement(11669..13009)
                     /gene="gntU"
                     /locus_tag="ACHKBL_002770"
     CDS             complement(11669..13009)
                     /gene="gntU"
                     /locus_tag="ACHKBL_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015128 - gluconate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035429 - gluconate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HFO5819622.1"
                     /translation="MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSG
                     MPLDKIAATMEKGMGGTLGFLAVVVALGAMFGKILHETGAVDQIAVKMLKSFGHSRAH
                     YAIGLAGLVCALPLFFEVAIVLLISVAFSMARHTGTNLVKLVIPLFAGVAAAAAFLVP
                     GPAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDISEPHL
                     GEGKMPSFGFSLSLILLPLVLVGLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLV
                     AIYGLAMRQGMPKDKVMEICGHALQPAGIILLVIGAGGVFKQVLVDSGVGPALGEALT
                     GMGLPIAITCFVLAAAVRIIQGSATVACLTAVGLVMPVIEQLNYSGAQMAALSICIAG
                     GSIVVSHVNDAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLS"
     gene            complement(13013..13540)
                     /gene="gntK"
                     /locus_tag="ACHKBL_002771"
     CDS             complement(13013..13540)
                     /gene="gntK"
                     /locus_tag="ACHKBL_002771"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HFO5819623.1"
                     /translation="MSTTNHDHHIYVLMGVSGSGKSAVASEVAHQLHAAFLDGDFLHP
                     RRNIEKMASGEPLNDDDRKPWLQALNDAAFAMQRTNKVSLIVCSALKKHYRDLLREGN
                     PNLSFIYLKGDFDVIESRLKARKGHFFKTQMLVTQFETLQEPGADETDVLVVDIDQPL
                     EGVVASTIEVIKKGK"
     gene            complement(13679..14674)
                     /gene="gntR"
                     /locus_tag="ACHKBL_002772"
     CDS             complement(13679..14674)
                     /gene="gntR"
                     /locus_tag="ACHKBL_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate operon transcriptional repressor GntR"
                     /protein_id="HFO5819624.1"
                     /translation="MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAAL
                     DELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYG
                     YKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSKSPCLDIAV
                     GFDNFEAARQMTTAIIARGHRHIAYLGARLDERTIIKQKGYEQAMLDAGLVPYSVMVE
                     QSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHG
                     HDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLDLGFTLSPGGSI"
     gene            complement(14898..15593)
                     /gene="yhhW"
                     /locus_tag="ACHKBL_002773"
     CDS             complement(14898..15593)
                     /gene="yhhW"
                     /locus_tag="ACHKBL_002773"
                     /EC_number="1.13.11.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quercetin 2,3-dioxygenase"
                     /protein_id="HFO5819625.1"
                     /translation="MIYLRKANERGHANHGWLDSWHTFSFANYYDPNFMGFSALRVIN
                     DDVIEAGQGFGTHPHKDMEILTYVLEGTVEHQDSMGNKEQVPAGEFQIMSAGTGIRHS
                     EYNPSSTERLHLYQIWIMPEENGITPRYEQRRFDAVQGKQLVLSPDARDGSLKVHQDM
                     ELYRWALLKDEQSVHQIAAERRVWIQVVKGNVTINGVKASISDGLAIWDEQAISIHAD
                     SDSEVLLFDLPPV"
     gene            complement(15716..16753)
                     /locus_tag="ACHKBL_002774"
     CDS             complement(15716..16753)
                     /locus_tag="ACHKBL_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFO5819626.1"
                     /translation="MVINCAFIGFGKSTTRYHLPYVLNRKDSWHVAHIFRRHAKPEEQ
                     APIYSHIHFTSDLDEVLNDPDVKLVVVCTHADSHFEYAKRALEAGKNVLVEKPFTPTL
                     AQAKELFALAKSKGLTVTPYQNRRFDSCFLTAKKAIESGKLGEIVEVESHFDYYRPVA
                     ETKPGLPQDGAFYGLGVHTMDQIISLFGRPDHVAYDIRSLRNKANPDDTFEAQLFYGD
                     LKAIVKTSHLVKIDYPKFIVHGKKGSFIKYGIDQQETSLKANIMPGEPGFAADDSVGV
                     LEYVNDEGVTVREEMKPEMGDYGRVYDALYQTITNGAPNYVKESEVLTNLEILERGFE
                     QASPSTVTLAK"
     gene            17087..17575
                     /gene="yhhY"
                     /locus_tag="ACHKBL_002775"
     CDS             17087..17575
                     /gene="yhhY"
                     /locus_tag="ACHKBL_002775"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HFO5819627.1"
                     /translation="MSEIVIRHAETRDYEAIRQIHAQPEVYCNTLQVPHPSDHMWQER
                     LADRPGIKQLVACIDGTVVGHLTIDVQQRPRRSHVADFGICVDSRWKNRGVASALMRE
                     MIEMCDNWLRVDRIELTVFVDNAPAIKVYKKFGFEIEGTGKKYALRNGEYVDAYYMAR
                     VK"
BASE COUNT         4336 a         4806 c         4419 g         4102 t
ORIGIN      
        1 attgtttatt gtgtatagaa ataccgtgag ttccactctg tattacttca gcggaacaca
       61 cggtatttag ccatatttga acttacaatc tcaccggatc aatatgccag atatgatcgg
      121 cgtactcttt gatcgtacgg tcagaagaga agtagcccat attggcaatg ttcagcatcg
      181 cttttgcggt ccactcttcc tgacgctcgt agagttcgtc gactttatcc tgacaatcga
      241 catagctgcg ataatccgcc agtacctggt agtgatcgcc gaagttgatc agcgaatcga
      301 ccagatcgcg atagcgaccc ggatcttccg gactgaatac accgctgccg atttgcgtca
      361 gcacctgatg cagctcctca tctttctcgt agtattcacg cggtttgtag ccctgacgac
      421 gcagttcttc cacttcttcc gctgtgttac caaagataaa gatattgtca gcaccgacat
      481 gatccagcat ctcgacattc gcaccgtcca gcgtaccgat agtcagcgcg ccgttaagcg
      541 caaacttcat gttactggtg ccggaagctt ccgtccccgc cagcgaaatc tgttcagaca
      601 gatcggccgc cggaatgatc aactgcgcca ggctaacact gtagttcggg atgaacacga
      661 ctttcagctt gtcgccaatc tgcggatcgt tgttgatcac tttcgctacg tcattgatca
      721 aatgaataat gtgcttcgcc atgtaatagg ccgaagccgc cttaccacca aaaatattca
      781 cgcgcggtac ccacttcgca tccgggtcgg ccttgatgcg gttatagcgg gtgatcacgt
      841 gcaacacatt catcaattga cgtttgtatt cgtgaatacg tttgatttgc acatcgaaca
      901 gcgctttcgg atttaccacc acattcagct gctgggcgat atactccgcc agacgctttt
      961 tgttctccag cttcgcctga tgcaccgcgt gattaaccat tgggaaatca cagtgttgtt
     1021 gcagatcatt aagcaggcta aggtcggtgc gccagttgcg gcccaggtgt tcgtccaaca
     1081 cggctgaaag cgatgggttc gctaccgcca gccagcgacg cggcgtcaca ccgttggtga
     1141 cgttggtgaa acgacccggg aagattttcg caaagtcggc aaatagcgat tgcaccatca
     1201 ggttagagtg cagctccgat acaccgttaa ctttgtggct caccacaacc gccagccagg
     1261 ccatacgcac acgacgaccg ttggattcat caatgatcga cgcccgtccc agcagatcgg
     1321 tatcgttcgg atactgttcc tgcaaggttt tcaggaaata gtcgttgatt tcaaagatga
     1381 tctgcaggtg acgcggcaga attttaccca gcatatcgac tggccaggtt tccagcgcct
     1441 cgctcattag cgtgtggttg gtgtaggaga agacctgaca acacacttca aacgcgtcgt
     1501 cccagctaaa ttggtgctca tcgatcagca gacgcatcat ctcaggaatc gacagcaccg
     1561 gatgagtatc gttgaggtga atcgcaattt tatccgccag gttatcgtag gttttatgca
     1621 actgataatg gcggcttaaa atgtcctgaa tggtcgagga aaccaggaag tattcctgac
     1681 gcagtcgcag ctcacgcccg gagtaggtgg agtcgtccgg atacagtacg cgagatacgt
     1741 tctcggagtg gtttttatct tccactgccg cgaagtagtc gccctggttg aatttaccga
     1801 ggttaatttc gctactggct tgggcactcc acaaacgcaa cgtgttggtc gcgtcggtgt
     1861 cgtaacctgg gattatctga tcgtaagcta ctcccagaat ctcttcggtt tcaatccagc
     1921 gcgttttttt accttcctgc tgaatgcgac cgccaaaacg gactttatag cgcgtgttgt
     1981 ggcgtttgaa ttcccacggg ttaccgtatt ccagccagta gtcaggcgac tctttctggc
     2041 taccgttaac gatgttctgc ttgaacatac cgtagtcata gcggatgccg taaccgcgcc
     2101 ccggcaaccc taacgtcgcc agagaatcga ggaagcaagc cgccagacgg cccaggccac
     2161 cgttaccgag gcccgggtca ttttcttcat caatcagctc ttcgagattt aatcccatcg
     2221 cttccagtgc gccctgtaca tcttcgtaaa ttcctagcga caacatggcg ttggaaagcg
     2281 tacggccaat caaaaactcc atcgacaggt agtaaacctg acgagtttct tgcgacaact
     2341 gggcacggtt tgagcgtaac cagcgctcca cgagacgatc gcgcacagca aataacgttg
     2401 cgttcagcca ttcatgttta ttggcgacga ccggatcctt gccaatcgta aacatcagct
     2461 tgtaagcgat agagtgctta agagcttcta cgctaagcgt gggcgatgaa tatgtaaacg
     2521 gagcattcat ataggcgttt cctgaaaact atttcaagcg atagtaaagc tcacggtacg
     2581 acttcgccgc gacctgccag ctaaaatcca ttgccatagc ctgacgttgc acaaaccgcc
     2641 acagtgaagg acgggaccac agtacaaaag cacgtcgaat agcccgtaac agcgaccagg
     2701 cattactatc ttcaaagaca aacccactgg cgacgccatc tgcaaggttc tcaagagaac
     2761 agtcagaaac cgtatcagca agcccaccgg tgcgccgcac taacggcagc gtaccgtact
     2821 tcaatccata aagttgcgtt aagccgcacg gttcaaaacg gctgggcacc agaatgacgt
     2881 ccgcgccgcc cataatgcga tgcgaaaatg cttcgtgata gccaatctga acgcccacct
     2941 ggccggggta ttccgctgcc gccgcaagga aaccttcctg cagcaccgga tcgcccgcgc
     3001 cgagtagcgc cagctgcccg ccctgctcca gaagaccagg taaggcttcc agcaccaggt
     3061 cgagaccttt ctggctagtc agacggctca ccactgcaaa aagcggcact ttatcgtcaa
     3121 ccttaagccc cattgcgatt tgtaactggc gcttattttc cgctttatct tccaacgtat
     3181 cgcgggtgta acgcgaggcc aacagtaagt ccgtctctgg actccagatt ttctcgtcca
     3241 cgccgttcag tacgccggaa agacgccctt cacggtgacg ctgttgcaac agaccttcca
     3301 taccgtaggc aaactgcggt tcggtgatct cgcgagcgta ggttgggctg actgccgtaa
     3361 tgtgatcggc atagtacaga ccggccttca gaaaagagat ttgtccgttg aattccagcc
     3421 catgaatatt aaagaatgac catggcaatt ggatgtcatt catgtgatgt gcataaaaca
     3481 tgccttgata ggccaggttg tgcacagtaa acaccgactt cgccggacgc ccgcgcgccg
     3541 ccagatacgc aggcgcaagg cctgcatgcc agtcgtgcgc atgcaccaca tcaggacgcc
     3601 agaatgggtc aagcccgctg gccatttctg ccccaaccca ccccagcagc gcaaaacgca
     3661 atacgttgtc ggtataggca aataagttgg tatcgtgata cgggcttccc ggacgatcat
     3721 agagatgcgg cgcgtcaatc aggtatatgc caaccccgtt gtaatgaccg aacaacagcg
     3781 tgatatgtcc ggcgaaggta tcacgacggg atactacctg cgcatcggtc acgccacggc
     3841 gaatatcggg aaatgcaggc aacagtacgc gagcgtcaac gccgtctgcg atttgtgctg
     3901 cgggtaatgc cccaataaca tcagccagac cgccggtttt aagcagcggg aacatctctg
     3961 aacatacatg taaaacctgc attatcgctc ctgtttatgc cctaacttcc gtagcatttc
     4021 gcgcgttacc agcacgatgc cttcttctga acgatagaaa cgacgtgcat cttcctctgc
     4081 gttttcacca atcaccatgc cttccggaat aacacaagca cgatcgatga cgcagcggcg
     4141 cagacggcac gagcgaccta cccatacttc cggtaacaat acggcggaat caatgttgca
     4201 gaatgaattc acgcgaacgc gcgagaacag aacggactgc accaccaccg aaccggagat
     4261 cacacaaccg ccggatacca gtgagttaag ggtcatccca tggctaccgg agcgatcctg
     4321 cacgaatttc gctggcggta atgattcatt gtaggtgcga attggccaat tgcgatcgta
     4381 catatccagt tccggcacca cagaggccag atcgaggttc gctttccagt aagcttccag
     4441 cgtacccaca tcgcgccagt acggctcggc atccgggtcg gattgtacgc aagagagcgg
     4501 gaacgggtgc gcataggcca gaccggcttc ggtgatcttg ggaatcaaat ctttgccaaa
     4561 gtcgtggctg gagttctcat cgcgatcgtc ttcttccagc agttcataca gatagtcggc
     4621 gtcaaagacg tagataccca tactcgccag agatttgctc ggatcgttcg gcattgacgg
     4681 tgggttagca ggtttttcaa cgaattcgat aattttatcg ttctcatcaa ccgccataac
     4741 gccaaatgcg gaggcttctt caatcggtac tggcatacaa gcaacggtgc aacgtgcgcc
     4801 tttttcgacg tggtcgataa gcatacgcga gtagtcttgc ttgtagatat ggtcgcccgc
     4861 caggatcacc acgtattccg ctttataacg gcggataata tcgaggtttt gggtaactgc
     4921 atctgcggtg ccgcgatacc agttttcccc tttcattctc tgctgtgctg gcagcagatc
     4981 gacaaactcg ttcatttctt cattgaagaa tgaccagccg cgctgaatgt gctgcaccag
     5041 agtgtgggac tggtactggg tgatcacgcc catacgacgg atcccggagt tgatgcagtt
     5101 agacagcgca aagtcgataa tgcggaactt accgccgaag tgtacggccg gttttgctcg
     5161 cttattggtt aaatccttca ggcgggtacc acgtcctccc gccagtatca gggcaacaga
     5221 tttcaatggc agctggcgcg ccaacattaa gtgatcgttc ttctctaaac taaccatgac
     5281 taactccttt tttatcatct ctggaacaca cacaatccgt gtgcaggtcc ctgccagaca
     5341 gccgtaatca ctgggttatc ctctccagcg aatgggggaa tggcgtgcca ctccccagca
     5401 ggtaaaacaa tctctgttac ctcaagcgtg gcgttaattg cgatcaaaaa gcgatccgag
     5461 agcagaattt gcagctgttt cggcccgttt tgccactcat ccgtgcttaa aggttgagca
     5521 tatcgattta gccaacggac attgccgtcg ccttcttccc accagcgatt ctccaccaaa
     5581 gcgggaatgc gcttgcgcag atggattaac gcggcggtaa atgcggttaa accactgctt
     5641 gcctgcgacc agtccaacca ggttaattgg ttatcctgac agtaggcatt gttattgcca
     5701 tgctggctgt gaccatgttc gtcaccggcc agtaacatcg gcgtaccctg ggagagcaac
     5761 aacgttgtta acagggcgtg aatgctgtcg cgacgccgtt caaccaggtc aagagtaccg
     5821 cctaaccctt ctttaccatg attgttactg taattgttgt tggtcccgtc gcgattttct
     5881 tctccgtttg cttcattgtg tttatggttg aagcaaacgc agtcgcgaag cgtaaaaccg
     5941 tcatgcgcgg tgacgagatt aatcgcggca ctcggcagac gaccattacg tttaaaaaca
     6001 tcgctggagg cagcaaaacg cccggcaaac gcccccagag gcaaatcata atgaagccag
     6061 aaacgacggg cagcatcgcg gaaatgatcg ttccactcgg caaacagcgg cgggaaattt
     6121 cccacctgat aaccaccagg agcaatatcc cacggttcag caattaactt cacctgcgag
     6181 agcaccgggc agttctggat agcggtaaac aacggcgcat cctgacggaa ctctggcgta
     6241 cggcccatga ctgccgccag atcaaagcgg aaaccatcga cgtggcaggt ttctacccaa
     6301 taacgcaggc aggcgctggc ataatccacc accgccggat gactcaaatt gagcgtgttg
     6361 ccacaaccgg tccagttgtg ataatcgccg tcttctctta tccaataata gctacggttg
     6421 tcgatcccac gcagagagaa taacgggccg tcgaggtcca gttccgcact atggttgagc
     6481 acgatatcaa gaatgacttc gatacccgct ttatgcagtg ctttgattgc atcgcgaaac
     6541 tcatccagcg ccgtttctgg ggagcaggca tacgccggat gcagcgcaaa catcgccacc
     6601 gggttataac cccagtagtt acttagaccc atacgttgca ggcgtggttc gctggcaaac
     6661 tgcgccaccg gcagcagttc cagcgcggta atgcccaact gtttcaaata gttgatcatc
     6721 accggatgcc ccagggcttt ataagtgcca cggatctcga ccgggatctc cgggtgcagg
     6781 tacgttaatc ctttgacatg ggcttcataa atgatggtac tgccccacgg cgtgcgcggc
     6841 ggagcatcat cctcccagtc atagtgatca accaccacta cgcatttcgg cgcaatggcg
     6901 gcgttgtcgc gatagtcagg ttcattatga ccggcgtgca gcagcgggtt atctttaaat
     6961 tccccgtcaa tttgccgcgc gcaaggatca atcaacaact tcgccgggtt aaagcgatgc
     7021 ccctcggcgg gttgccaggg gccatgaacg cgataaccat aacgcaaacc cggacgcgca
     7081 tccggcagat aaccgtgcca aatgtcgcca ctgtgccctg gcaagtcata gcgatgttcc
     7141 tggccattgg cgtcaaagac acacagttct acccgctcgg catgagcgga gaaaagtgtg
     7201 aagttgacgc cctgaccgtc gtaatgcgcg ccgaggggtg cgggtttgcc aatggcgagt
     7261 tgtgtcattc tgcctcccga accagccaga tagtggccag cggtggtagt gtcaggctta
     7321 atgaatgctg acgaccgtgg ctggcaatct catcgctgtg taccgtgccg ccattgccga
     7381 cattactgcc gtgatagtgc atggaatcgg tattgaggat ttcacgccat ttgcccggct
     7441 ggttaatgcc gaagcgataa tcatgacgcg gcaccggagt aaagttactg gcaacgatga
     7501 tttcgttacc ctctttatcg cgacgcacaa agatcagcac cgagcgttct ttgtcatcca
     7561 ccaccagcca ttcaaaaccg tacgggtcaa aatccagttc atgcatcgct ttatggtggc
     7621 ggtaggtgag gttcagatcg cgcaccagac gctggacacc gtggtgccag ttatcgccgc
     7681 cttccaacag atgccagtcg aggctggcgt catggttcca ctcgcggccc tgggcaaatt
     7741 cgttccccat gaacagcagt ttcttgcccg ggaatgccca catccagccg tagtaggcgc
     7801 gcaggttagc gaatttctgc catgcgtcgc ccggcatccg gtcgagaatc gattttttac
     7861 cgtggaccac ttcatcatgc gacaacggca ggacgaagtt ttcagtgtag ttgtagagca
     7921 tcccgaaggt cagtttatcg tgatgatact gacgataaac cgggtcgagc ttcatgtagt
     7981 ccagggtgtc atgcatccag ccgaggttcc acttgtacca gaagcccaga ccgcccatat
     8041 cctgcggacg agaaacgcca gggaaatcgg tagactcctc agccattgtc accgcaccgg
     8101 aaacctgctc accaagaata cggttggtat tacgcaagaa ttcaatcgct tcaagattct
     8161 cgcgcccgcc aaattcgttc gggatccact ccccctcttt acggctgtag tcgcgataaa
     8221 tcattgacgc taccgcatcg acgcgcagcg catcaatacc aaaacgttca atccagtaaa
     8281 gcgcgttacc gacaaggaag ttactgactt cacggcgacc atagttgtag atcagcgtgt
     8341 tccagtcctg atgatagcct tcgcgcggat cgctgtgttc atacaagttc gtgccatcaa
     8401 attcggcaag cgcaaagtca tcggtcggga agtggcctgg cacccagtcg agaatcacgt
     8461 tcagaccagc tgcgtgtgcg gcatcaatga aataacggaa gtcgtcgcga gtaccaaaac
     8521 ggcgggtcgg tgcatacaga ccggttggct gataacccca actgccatcg aagggatgct
     8581 cgttaatggg cagtagttcg aggtgggtaa agcccatcca tttagcataa ggcaccagtt
     8641 gatcggccag ctcgcggtag ctcaaccaga aattgttgtc ggtgtggcga cgccaggagc
     8701 ccagatgaac ttcataaata gagattggcg catcaaactg attcgctttt ttgcgctctt
     8761 cagtccgtac aaccttttcc ggcagcccgc aaataagaga cgcggtttcc gggcgcattt
     8821 gcgcttcgaa ggcataaggg tcggacttca gacgcaagtt gccattggca tcaatcatct
     8881 cgtatttata gagctgaccg ttatgcgccc cagggataaa cagttcccag atgccgctct
     8941 ctttacgcag gcgcatcggg tgacggcgac cgtcccagta gttgaattgc ccaaccaccg
     9001 agacccgacg ggcgtttgga gcccagacag agaaacgcgt acctgtgacg ccatccatag
     9061 tatctgcatg cgcgcctaag gtttcatacg ggcgcaggtg agtaccttca gataatagcc
     9121 aggcatccat ttcctggatt agcggaccaa aacggtaagg atcatcaatc aggttttgct
     9181 gaccatgcca gacaacagcc aactgatagc ggaaaaaatt cttacgtcgc ggaatgacgc
     9241 cgctaaagaa tccccgtgag tcgagacact ccagttttgc gagtttgcgc ccggttttcg
     9301 gttcaatcac ccacacatcg gtagcgtcgg gtaaaagggc acggacttcc agtcccgcgg
     9361 tggttttatg cattcccagt acggaaaaag gatccgcaaa atggcctgca attagcgcgt
     9421 taatcacgtc tctatcgata cgatcggaca tgcttgtctt cctgttttat tgtgtcaccc
     9481 catccaattg gatttttgac ttctggttgt gacatttttt tgacctgaac ggcgcagcac
     9541 tctgtgcatc ctctctgcgt cgtcctcact tcaggtaagg ctgtgaatac tcatgtattc
     9601 agccaccctt aaagaatagc caatgctcta tttaactccc ggtaaatcat gaaacatctg
     9661 cgcttactcc tgtattacgc actaacaggg gcggcatcgc gccccagatt taatgaataa
     9721 agattacgcc agttgacgaa gcatccgacg caacggctcc gcggcccccc acagcagctg
     9781 gtcacccacg gtaaaggctg acaggaactc cggtcccata ttcagcttac gcaggcggcc
     9841 taccggcgtg gtcagcgtgc cggtaacggc agctggggtt agctcacgca tagtgatttc
     9901 ccgatcgttc ggaacgactt tcgcccacgg attgtgtgca gccagcagtt cttccacggt
     9961 cggaatagac acatcttttt tcaatttaat agtgaatgcc tggctgtggc agcgcaatgc
    10021 cccgacacgc acacataaac catctaccgg aattacggaa gatgtgttga ggatcttgtt
    10081 ggtttccgcc tgccctttcc actcttcgcg gctctgaccg ttatcgagct gtttgtcgat
    10141 ccacggaatc aggctacccg ccagcggcac gccaaagtta tccaccggca gctcaccgct
    10201 acgggttaag gttgtgactt tgcgttcgat atcgagaata gcagaggacg gggtcgcgag
    10261 ttcatctgcc acatggccat acagatggcc catctgggtt aataactcac gcatatgtcg
    10321 cgcaccaccg ccggaagcgg cctggtaggt tgcaacggac acccaatcaa caagatcatt
    10381 ggcgaataaa ccacccaacg acatcaacat caggcttacg gtacagttac cgccaacaaa
    10441 agtcctgatg ccattattta atccatcggt aatgacgtcc tgattgacgg ggtcaagaat
    10501 gatgatggca tcatctttca tgcgcagaga cgatgctgcg tcaatccagt aaccttgcca
    10561 tccgctttca cgaagctttg gatagatttc gttggtataa tcgccgccct gacaggtcac
    10621 aatgatatcg agggccttta gcgcctccag atcaaaggca tcctgaagtg tgccagtggt
    10681 tccgccaaaa gacggcgcag cctgaccaag ctgagaagta gaaaagaaga cagggcgaat
    10741 ggcgtcgaag tcgcgctctt caaccatgcg ttgcatgaga acggagccga ccataccgcg
    10801 ccagccgata aaaccaacat ttttcataag cgttttttcc tgcaaagatg tgtgctgtat
    10861 aaatgtgccg gtctcctctt ggcacatctt tcaccataca aaaagcagcc aaagtcgcaa
    10921 gtgaaattaa tcaatgatag cgaagccatc agtaatgcga cttatcctgc tttgttagca
    10981 cgcagaaagt ccgcggcaat tatcagggaa tttgagttat gaatgaaatc atttctgcag
    11041 cagttttatt gatcctgatt atggatccgc tcggaaacct acctattttc atgtccgtac
    11101 tgaaacatac tgaaccgaaa agacggcggg caatcatggt gcgagagttg cttattgctc
    11161 tcctggtgat gctggtgttc ctgtttgcgg gtgagaaaat tctggcattt ctcagcctgc
    11221 gagcagaaac cgtctccatt tctggcggca tcattctgtt tctgatcgcc attaagatga
    11281 ttttccccag cgcttcagga aatagcagcg ggcttccggc aggtgaagag ccgtttatcg
    11341 tgccgttggc aattccgtta gtcgccgggc cgactattct cgccacgctg atgttgttgt
    11401 ctcatcagta cccgaatcag atgggacatc tggtgattgc tctgctgctg gcctggggcg
    11461 gcacctttgt catcctgcta cagtcttcgc tatttttacg tctgctgggc gagaaagggg
    11521 tgaacgcact tgaacgcctg atgggattga ttctggtgat gatggcaacc cagatgttcc
    11581 tcgacggcat tcgaatgtgg atgaaggggt aacgaggaga gtttttcgaa aagagcccgg
    11641 tagcgtcaga actaccgggt aaacaaactt aactcaacag ctggaacgca atcatcccaa
    11701 cgatggcacc gacagtgccg aggatggttt ccatcatggt ccaggttttc agcgtttcgg
    11761 cttcggtcgc gccggtaaat ttaccgaaca accagaaacc ggcgtcgtta acgtggctga
    11821 caacaatcga accaccggcg atacaaatcg acagcgccgc catttgcgca ccagagtagt
    11881 tcagttgttc aatgactggc atcaccagtc ccaccgccgt taaacaggct acggtggcag
    11941 acccctgaat gatgcgcact gcagctgcca gcacgaagca ggtgatagca atcggcaggc
    12001 ccatgccggt taacgcttcg cccagtgccg gacctacgcc agagtcaacc agcacctgtt
    12061 tgaacacgcc gcccgcacca atcaccagca gaatgatccc cgccggttgc agcgcgtgac
    12121 cgcaaatctc catcactttg tcttttggca tgccctgacg cattgccagg ccataaatcg
    12181 ctaccagaca agcaaccaga atcgcggtaa acggatgacc aataaactcg aaccattcgt
    12241 aagcggtaga gccttctggc acaaaacgcg cggcaatggt tttcagcccc accagcacca
    12301 gcggcaacag gatcagcgac aggctgaatc cgaaagatgg cattttacct tcgccgagat
    12361 gcggttcgct gatgtcgtca ggaatatgca gctcaacgta acggctgatg aagttacccc
    12421 acagcggccc ggcaataatc attcccggaa ttgccgcaca caggccaatc aggatcatcc
    12481 agccaaagtc ggcgttcatc tgcgatgcca gcagcattgg cgctggcccc ggcaccagga
    12541 acgcagccgc tgccgccacg cctgcaaata atgggattac cagcttcacc aggttcgtac
    12601 cggtatgacg cgccattgag aaagcaacgc taatcagcag aacaatcgcc acttcaaaga
    12661 acagcggtag cgcacaaacc agccccgcaa ggccaatggc ataatgcgcg cggctgtgac
    12721 cgaaggattt aagcattttg acggcaatct gatcgactgc gccggtttca tgtaagatct
    12781 tgccaaacat agctcccagg gcgacaacca ccgccaggaa gccgagggtg cctcccatcc
    12841 ctttttccat cgtcgctgcg attttatcga gcggcatacc ggaaaaaagg ccagccccca
    12901 tggacaccac cattaaagcc aggaaagcgt gcatacgcgc cttcatgact aaaaacagca
    12961 gcagtaaaac agaccctact gctgttaaaa caagcgttaa tgtagtcact acttatttgc
    13021 cttttttaat aacctcaatg gtgcttgcca caacaccttc cagcggttga tcgatatcca
    13081 ccaccagtac atcggtttcg tccgcacccg gctcctgcag cgtttcaaac tgcgtcacca
    13141 acatttgggt tttaaagaaa tggcctttgc gcgctttcag gcggctttca atcacatcaa
    13201 aatcaccttt caggtagatg aaagagagat tcggattacc ttcacgcagc aagtcgcgat
    13261 agtgtttttt caatgcagaa cagacgatca gtgacacttt attagtgcgc tgcatagcaa
    13321 acgcggcgtc gttcagcgcc tgcagccacg gtttgcgatc gtcatcattc agcggttcgc
    13381 cagacgccat tttttcgata ttgcgccgtg gatggaggaa atcgccatca agaaacgcgg
    13441 catgaagttg atgcgccact tcacttgcga ccgcagattt gccgctgccc gatacgccca
    13501 tcaagacgta aatgtggtga tcatggttag tcgtgctcaa agtggtgccc ccacaataca
    13561 agaattaaaa atgttacggg taactgttat cggtaacatt gtccagccgg acaatatcag
    13621 aagcaatatc catccgtgcc ttaagtgtat aagtgtgagc tacttcaaat ttgtgggctt
    13681 aaatagatcc gcccggtgac aaggtgaaac ctaaatctaa cattttcggt gtcacagatt
    13741 cgccacgaat acgcgccagc aggcgttcag cgccaatact gcccatccgc tcacgcggcg
    13801 tcaggacgct cgccaaccgt ggctccatca cctggccaat gtcatgaccg tggaaaccgg
    13861 caatcgccat atcgtcagga actttcaacc ccagacgctg acattcaaac gccgcgccga
    13921 ccgccaggtc atcattagta cagaacacgc catccagctg cggatattcc cgccgtgcct
    13981 ggcgaatcag ttcaataccg gaagaataag aagaagattg ctcaaccatc acgctatacg
    14041 gcaccaggcc tgcatccagc atcgcctgtt catatccctt ctgtttgatg atagtacgtt
    14101 cgtcgagacg tgcgccgaga taggcaatgt ggcgatgccc gcgagcaata atggctgtgg
    14161 tcatctggcg tgccgcttca aagttatcaa aaccgacggc gatatcgagg caaggcgact
    14221 tgctgtccat cagttccacc accgggatcc ccgccacttc aatcatcttt aaggtgcgcg
    14281 gcgtgtgggt acgttcggtg aggatcagtc cgtcgatatt ccatgagagc atggattcga
    14341 gacgttcttg ctccatttcc ggtttataac cgtagtgggc cagcatggtc tgataaccgt
    14401 gcgcgtcggt gacgctttcg attccgcgta atacttccgc gaaaacctgg ttggtgagag
    14461 aaggtaacag gacgccaatc gcccggctgg tggcgttaga gaggatatcg ggtgcgcgat
    14521 tgggaatata acccagttca tcaagagcgg cggcaatctt gccgcgtaga gcgacggaaa
    14581 cctgctccgg gttacgcaaa aagcggctga ccgtcatttt ggtcacgcct acacggtcag
    14641 ccacatcctg aagtacgggt cttttctttt tcatcgtcct gaaggtacaa aagagataga
    14701 ttctcttagt ttaacacgga cgcatcagaa ccttcccggg gaaaacagtg cttgtttaca
    14761 attattagat atacactaaa tcaattgcat cgcattgtgc taacgacgcg gcaaaacccc
    14821 ggaagcgtac atcagcacgc gaccggagcg agcgaggaag cctgcaaaaa ggcaggcttc
    14881 aaagatgccg ttgagcttta aaccggcggc agatcgaaca gtaacacttc gctgtcgcta
    14941 tccgcatgga tggagattgc ctgctcatcc cagattgcca gaccatcgct gatcgaggct
    15001 ttcacgccgt taatggtgac attgcctttc accacctgga tccagacgcg gcgttcagcg
    15061 gcaatctgat gcaccgactg ctcatctttc agcaacgccc agcggtacag ttccatatcc
    15121 tgatgcactt tcaatgaacc atcacgcgca tctggcgaaa gcaccagttg tttgccctgc
    15181 actgcatcga agcgacgctg ttcataacgc ggcgtaatgc cgttttcttc cggcatgatc
    15241 cagatctgat acaggtgcag acgctcggtg ctgcttgggt tgtactctga gtgacgaata
    15301 cccgtacccg cactcataat ctggaactcg cccgccggaa cctgctcttt attgcccatg
    15361 ctgtcctggt gctcaacagt accttccagc acgtaggtca aaatttccat atctttatgc
    15421 gggtgagtgc cgaagccctg ccctgcttca atcacgtcgt cgttaatcac gcgcagcgcg
    15481 gagaagccca taaagttcgg atcgtaatag ttggcaaaag agaaagtatg ccaggagtcc
    15541 agccagccat gatttgcatg accacgttca tttgctttgc gtaagtagat catttgtatt
    15601 cgccccctga atgatttcga tgggcttagt gtggacccga tctgcctggg atgatagagg
    15661 gtgaaaattg acccctctgt tcaaaaaaat tgaactattc gaggggccat caaacttact
    15721 tcgcgagagt cactgtggag ggagaggctt gctcaaatcc gcgttcaagg atttccagat
    15781 tggtaagaac ttcagattcc ttgacgtaat ttggcgcacc gttggtgatg gtttgataca
    15841 acgcatcata aacgcgcccg taatcgccca tctccggctt catctcttct ctgaccgtca
    15901 caccctcgtc attgacatac tccagcacac cgaccgaatc atccgctgcg aatcccggtt
    15961 cgcccggcat aatattagcc ttgaggctgg tttcctgctg gtcgataccg tatttaataa
    16021 acgaaccttt cttaccgtga acgataaatt tcggataatc gattttcacc agatggctgg
    16081 ttttgacgat ggctttcaag tcgccataaa acagttgcgc ttcaaaggtg tcgtcaggat
    16141 tggctttatt gcgcaggctg cggatgtcat aagcgacgtg atccgggcga ccgaacagag
    16201 aaataatctg gtccatcgta tgcacaccaa ggccatagaa cgcgccatcc tgcggcaacc
    16261 caggtttggt ttctgccacc gggcggtaat agtcaaaatg gctttccact tcaacaatct
    16321 ctcccaactt gccgctttca atcgcttttt tcgccgtcag gaagcaggaa tcaaagcgac
    16381 gattctgata cggcgtgacg gtcagccctt tgcttttcgc cagcgcaaac agctccttcg
    16441 cctgggcaag cgtcggggtg aacggttttt cgaccagcac atttttcccg gcttccagcg
    16501 cgcgtttcgc gtattcgaaa tggctgtccg cgtgggtgca gacgacaacc agcttaacat
    16561 cgggatcgtt tagcacttcg tcgagatcgc tggtgaaatg gatatgagag taaatgggag
    16621 cctgttcttc cggctttgca tggcggcgaa aaatatgcgc gacatgccag ctatccttgc
    16681 ggttaagtac atacggcaga tggtaacggg tggtgctttt gccgaagcca ataaaggcgc
    16741 agttgatgac catgatttcg tcctttttaa ggtggttatt tacaccttag cgcaaagcgg
    16801 acgtggttcc tactggagtg cgcataacga acacaagcac ttcccgagga taaattgaga
    16861 acgaaagatc aaaaaaaaag ccagcacccg gctggctaag taatactgga agcaatgtga
    16921 gcaatgtcgt gctttcaggt tctccgcgag ggtcttcctg atcgcgagac aataataatc
    16981 attctcattc gcacttgtcc aacacttttt gcaaaaaaat gcatttgact cgcattcgaa
    17041 agtcaatgat gttgaaaggg acatttaccc caaagaggac aaaggaatga gtgagatagt
    17101 gatacgccac gcagaaacac gggattacga ggccatcagg cagattcacg cccagccgga
    17161 ggtgtattgc aacacactac aggtgcctca tccctccgat catatgtggc aggaacgact
    17221 cgccgatcgc cccggcatca agcaactcgt cgcctgtatt gatggaaccg tagtaggtca
    17281 tctcaccatt gatgtacagc aacgcccgcg ccgcagtcat gttgccgatt ttggtatctg
    17341 tgtcgactcc cgttggaaga accgcggcgt cgccagcgcc ctgatgcgag agatgattga
    17401 aatgtgcgac aactggttgc gggtagatcg tattgagcta acggtgttcg tcgataacgc
    17461 gccagcaatt aaggtctata aaaaatttgg ctttgagatt gaagggactg gcaagaagta
    17521 cgcattacgt aatggtgaat atgtcgatgc gtattatatg gcgcgggtga agtaagatag
    17581 tgcccttttt ctgagatgga aaaagggtgt cattcaaaat cggcatacct tcctttaagg
    17641 tatttatttt cccaatacat ata
//
LOCUS       DAZFLD010000099        17616 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.100, whole genome shotgun sequence.
ACCESSION   DAZFLD010000099 DAZFLD010000000
VERSION     DAZFLD010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17616
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.100"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            254..1669
                     /gene="tnaA"
                     /locus_tag="ACHKBL_002776"
     CDS             254..1669
                     /gene="tnaA"
                     /locus_tag="ACHKBL_002776"
                     /EC_number="4.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009034 - tryptophanase activity [Evidence
                     IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HFO5819628.1"
                     /translation="MENFKHLPEPFRIRVIEPVKRTTRAYREEAIIKSGMNPFLLDSE
                     DVFIDLLTDSGTGAVTQSMQAAMMRGDEAYSGSRSYYALAESVKNIFGYQYTIPTHQG
                     RGAEQIYIPVLIKKREQEKGLDRSKMVAFSNYFFDTTQGHSQINGCTVRNVYIKEAFD
                     TGVRYDFKGNFDLEGLERGIEEVGPNNVPYIVATITSNSAGGQPVSLANLKAMYSIAK
                     KYDIPVVMDSARFAENAYFIKQREAEYKDWTIEQITRETYKYADMLAMSAKKDAMVPM
                     GGLLCMKDDSFFDVYTECRTLCVVQEGFPTYGGLEGGAMERLAVGLYDGMNLDWLAYR
                     IAQVQYLVDGLEEIGVVCQQAGGHAAFVDAGKLLPHIPADQFPAQALACELYKVAGIR
                     AVEIGSFLLGRDPKTGKQLPCPAELLRLTIPRATYTQTHMDFIIEAFKHVKENAANIK
                     GLTFTYEPKVLRHFTAKLKEV"
     gene            1761..3008
                     /gene="tnaB"
                     /locus_tag="ACHKBL_002777"
     CDS             1761..3008
                     /gene="tnaB"
                     /locus_tag="ACHKBL_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity tryptophan permease TnaB"
                     /protein_id="HFO5819629.1"
                     /translation="MTDQSEKKHSAFWGVMVIAGTVIGGGMFALPVDLAGAWFFWGAF
                     ILIIAWFSMLHSGLLLLEANLNYPVGSSFNTITKDLIGNTWNIISGITVAFVLYILTY
                     AYISANGAIISETISMNLGYHANPRIVGICTAIFVASVLWISSLAASRITSLFLGLKI
                     ISFVIVFGSFFFQVDYSILRDATSTTAGTSYFPYIFMALPVCLASFGFHGNIPSLIIC
                     YGKRKDKLIKSVVFGSLLALVIYLFWLYCTMGNIPRESFKAIISSGGNVDSLVKSFLG
                     TKQHGIIEFCLLVFSNLAVASSFFGVTLGLFDYLADLFKIDNSHAGRFKTVLLTFLPP
                     ALLYLIFPNGFIYGIGGAGLCATIWAVIIPAVLAIKARKKFPNQMFTVWGGNLIPAIV
                     ILFGITVILCWFGNVFNVLPKFG"
     gene            3140..4315
                     /gene="mdtL"
                     /locus_tag="ACHKBL_002778"
     CDS             3140..4315
                     /gene="mdtL"
                     /locus_tag="ACHKBL_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtL"
                     /protein_id="HFO5819630.1"
                     /translation="MSRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAF
                     SVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFIIASVFCSLAETSALFLAGRFLQG
                     LGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLF
                     WTMATMGIAVLMLSLFILKETRPAAPTTSDKPRENSESLLNRFFLSRVVITTLSVSVI
                     LTFVNTSPVLLMEIMGFERGEYATIMALTAGVSMTVSFSTPFALGIFKPRTLMITSQV
                     LFLAAGITLAVSPSHAVSLFGITLICAGFSVGFGVAMSQALGPFSLRAGVASSTLGIA
                     QVCGSSLWIWLAAVVGIGAWNMLIGILIACSIVSLLLIMFVAPGRPVAAHEEIHHHA"
     gene            4290..5249
                     /gene="yidZ"
                     /locus_tag="ACHKBL_002779"
     CDS             4290..5249
                     /gene="yidZ"
                     /locus_tag="ACHKBL_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator YidZ"
                     /protein_id="HFO5819631.1"
                     /translation="MKKSITTLDLNLLLCLQLLMQERSVTKAAKRMNVTPSAVSKSLA
                     KLRAWFDDPLFVNSPLGLSPTPLMVSMEQNLAEWMQMSNLLLDKPHHQTPRGLKFELA
                     AESPLMMIMLNALSKRIYQRYPQATIKLRNWDYDSLDAITRGEVDIGFSGRESHPRSR
                     ELLSSLPLAIDYEVLFSDVPCVWLRKDHPALHEAWNLDTFLRYPHISICWEQSDTWAL
                     DNVLQELGRERTIAMSLPEFEQSLFMAAQPDNLLLATAPRYCQYYNQLHQLPLVALPL
                     PFDESQQKKLEVPFTLLWHKRNSHNPKIVWLRETIKNLYASMA"
     gene            5406..6155
                     /locus_tag="ACHKBL_002780"
     CDS             5406..6155
                     /locus_tag="ACHKBL_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HFO5819632.1"
                     /translation="MATHFARGILTEGHLISVRLPSQCHQEARNIPPHRQSRFLASRG
                     LLAELMFMLYGIGELPEIVTLPKGKPVFSDKNLPSFSISYAGNMVGVALTTEGECGLD
                     MELQRATRGFHSPHAPDNHTFSSNESLWISKQNDPNEARAQLITLRRSVLKLTGDVLN
                     DDPRDLQLLPIAGRLKCAHVNHVEALCDAEDVLVWSVAVTPTIEKLSVWELDGKHGWK
                     SLPDIHSRANNPTSRMMRFAQLSTVKAFSPN"
     gene            6177..6743
                     /gene="yieF"
                     /locus_tag="ACHKBL_002781"
                     /gene_synonym="chrR"
     CDS             6177..6743
                     /gene="yieF"
                     /locus_tag="ACHKBL_002781"
                     /gene_synonym="chrR"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I chromate reductase YieF"
                     /protein_id="HFO5819633.1"
                     /translation="MSEKLQVVTLLGSLRKGSFNGMVARTLPKIAPASMEINALPSIA
                     DIPLYDADVQQEDGFPATVEALAEQIRQADGVVIVTPEYNYSVPGGLKNAIDWLSRLP
                     DQPLAGKPVLIQTSSMGVIGGARCQYHLRQILVFLDAMVMNKPEFMGGVIQNKVDPQT
                     GEVIDQGTLDHLTGQLTAFGEFIQRVKI"
     gene            complement(6797..8134)
                     /gene="adeP"
                     /locus_tag="ACHKBL_002782"
     CDS             complement(6797..8134)
                     /gene="adeP"
                     /locus_tag="ACHKBL_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeP"
                     /protein_id="HFO5819634.1"
                     /translation="MSQQHTTQASGQGMLERVFKLREHGTTARTEVIAGFTTFLTMVY
                     IVFVNPQILGVAGMDTSAVFVTTCLIAAFGSIMMGLFANLPVALAPAMGLNAFFAFVV
                     VQAMGLPWQVGMGAIFWGAIGLLLLTIFRVRYWMIANIPVSLRVGITSGIGLFIGMMG
                     LKNAGVIVANPETLVSIGNLTSHSVLLGILGFFIIAILASRNIHAAVLVSIVVTTLLG
                     WMLGDVHYNGIVSAPPSVMSVVGHVDLAGSFNLGLAGVIFSFMLVNLFDSSGTLIGVT
                     DKAGLADEKGKFPRMKQALYVDSISSVTGSFIGTSSVTAYIESSSGVSVGGRTGLTAV
                     VVGLLFLLVIFLSPLAGMVPGYAAAGALIYVGVLMTSSLARVNWQDLTESVPAFITAV
                     MMPFSFSITEGIALGFISYCVMKIGTGRLRDLSPCVIIVALLFILKIVFIDAH"
     gene            8301..8966
                     /gene="yieH"
                     /locus_tag="ACHKBL_002783"
     CDS             8301..8966
                     /gene="yieH"
                     /locus_tag="ACHKBL_002783"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate phosphatase"
                     /protein_id="HFO5819635.1"
                     /translation="MSQIEAVFFDCDGTLVDSEVICSRAYVTMFREFGIHVDLEEIFT
                     RFKGVKLYEIIDIISKEQGVTMVQADAEHVYRAEVARLFDTELEDIAGANELLASINV
                     PMCVVSNGPVSKMQHSLGKLNMLHYFPEKLFSGYDIQRWKPDPALMFHAAKAMNVNVE
                     NCILVDDSSAGAQSGIDAGMEVFYFCADPHNKPIVHPKVTTFTHLSQLPELWKARGWD
                     ITA"
     gene            9033..9506
                     /locus_tag="ACHKBL_002784"
     CDS             9033..9506
                     /locus_tag="ACHKBL_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CbrB"
                     /protein_id="HFO5819636.1"
                     /translation="MSVSRRVIHHGLYFAVLGPLIGVLFLVLYIFFAKEPLILLVIIQ
                     VLPLFLLMSITTGAIPAMLTGVMVACLPEKIGSQKRYRCLVGGIGGVVITEIYCAVIV
                     HIKDMASSALFENILSGENLVVRIIPALLAGVVMSRIITHLPGLDISCPETDSLS"
     gene            complement(9588..10310)
                     /locus_tag="ACHKBL_002785"
     CDS             complement(9588..10310)
                     /locus_tag="ACHKBL_002785"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFO5819637.1"
                     /translation="MKLIITEDYQEMSRVAAHHLLGYMSKMRRVNLAITAGSTPKGMY
                     EYLITLVKGKPWYDNCYFYNFDEIPFRGKEGEGVTITNLHNLFFTPAGIKEENIQKLT
                     IDNYREHDQKLAREGGLDLVVLGLGVDGHFCGNLPNTTHFHEQTVEFPIQGEMVDIVA
                     HGELGGDFSLVPDSYVTMGPKSIMAAKNLLIIVSGAGKAQALKNVLQGPVTEDVPASV
                     LQLHPSLIVIADKAAAAELALG"
     gene            complement(10325..11494)
                     /locus_tag="ACHKBL_002786"
     CDS             complement(10325..11494)
                     /locus_tag="ACHKBL_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418175.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HFO5819638.1"
                     /translation="MNIKIAALTLAIASGISAQWAIAADMPASPAPTIPVKQYVTQVN
                     TDNSVTFRYFAPGAKNVSVVVGVPVPDNIHPMTKDEAGVWSWRTPALKGNLYEYFFNV
                     DGVRSIDTGTAMTKPQRQVNSSMILVPGSYLDTRSVVHGDLIAITYHSNALQSERQMY
                     VWTPPGYTGIGEPLPVLYFYHGFGDTGRSAIDQGRIPQIMDNLLAEGKIKPMLVVIPD
                     TETDAKGIIPEDFVPQERRKVFYPLNAKAADRELMNDIIPLISKRFNVRKDADGRALA
                     GLSQGGYQALVSGMNHLESFGWLATFSGVTTTTVPDEGVAARLNEPAAINQQLRNFTV
                     VVGDKDVVTGKDIAGLKTELEQKKIKFDYQEYPGLNHEMDVWRPAYAAFVQKLFK"
     gene            complement(11521..13137)
                     /gene="bglH"
                     /locus_tag="ACHKBL_002787"
     CDS             complement(11521..13137)
                     /gene="bglH"
                     /locus_tag="ACHKBL_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-specific porin BglH"
                     /protein_id="HFO5819639.1"
                     /translation="MFRRNLITSAILLMAPLAFSAQSLAESLTVEQRLELLEKALRET
                     QSELKKYKDEEKKKYTPATVNRSVSTNDQGYAANPFPTSRAAKPDAVLVKNEEKNASE
                     TGSIYSSMTLKDFSKFVKDEIGFSYNGYYRSGWGTASHGSPKSWAIGSLGRFGNEYSG
                     WFDLQLKQRVYNENGKRVDAIVMMDGNVGQQYSTGWFGDNAGGENFMQFSDMYVTTKG
                     FLPFAPEADFWVGKHGAPKIEIQMLDWKTQRTDAAAGVGLENWKVGPGKIDIALVRED
                     VDDYDRSLQNKQQINTNTIDLRYKDIPLWDKATLMVSGRYVSANESASEKDNQDNNGY
                     YDWKDTWMFGTSLTQKFDKGGFNEFSFLVANNSIASNFGRYAGASPFTTFNGRYYGDH
                     TGGTAVRLTSQGEAYIGDHFIVANAIVYSFGNDIYSYETGAHSDFESIRAVVRPAYIW
                     DQYNQTGVELGYFTQQNKDANSNKYNESGYKTTLFHTFKVNTSMLTSRPEIRFYATYI
                     KALENELDGFTFEDNKDDQFAVGAQAEIWW"
     gene            complement(13223..14617)
                     /gene="bglB"
                     /locus_tag="ACHKBL_002788"
     CDS             complement(13223..14617)
                     /gene="bglB"
                     /locus_tag="ACHKBL_002788"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglB"
                     /protein_id="HFO5819640.1"
                     /translation="MKAFPETFLWGGATAANQVEGAWQEDGKGITTSDLQPHGVMGKM
                     EPRILGKENIKDVAIDFYHRYPEDIALFAEMGFTCLRISIAWARIFPQGDEAEPNEAG
                     LAFYDRLFDEMAQAGIKPLVTLSHYEMPYGLVKNYGGWANRAVIDHFEHYARTVFTRY
                     QHKVVLWLTFNEINMSLHAPFTGVGLAEESGEAEVYQAIHHQLVASARAVKACHSLIP
                     EAKIGNMLLGGLVYPLTCQPQDMLQAMEENRRWMFFGDVQARGQYPGYMQRFFRDHNI
                     TIEMTESDAEDLKHTVDFISFSYYMTGCVSHDESINKNAQGNILNMIPNPHLKSSEWG
                     WQIDPVGLRILLNTLWDRYQKPLFIVENGLGAKDSVEVDGSIQDDYRIAYLNDHLVQV
                     NEAIADGVDIMGYTSWGPIDLVSASHSQMSKRYGFIYVDRDDNGEGSLTRTRKKSFGW
                     YAEVIKTRGLSLKK"
     gene            complement(14636..16510)
                     /gene="bglF"
                     /locus_tag="ACHKBL_002789"
     CDS             complement(14636..16510)
                     /gene="bglF"
                     /locus_tag="ACHKBL_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIABC"
                     /protein_id="HFO5819641.1"
                     /translation="MTELARTIVAGVGGADNIVSLMHCATRLRFKLKDESKAQAEVLK
                     KTPGIIMVVESGGQFQVVIGNHVADVFLAVNSVAGLGEKAQQAPENDDKGNLLNRFVY
                     VISGIFTPLIGLMAATGILKGMLALALTFQWTTEQSGTYLILFSASDALFWFFPIILG
                     YTAGKRFGGNPFTAMVIGGALVHPLILTAFENGQKVDALGLDFLGIPVTLLNYSSSVI
                     PIIFSAWLCSILERRLNAWLPSAIKNFFTPLLCLMVITPITFLLVGPLSTWISELIAA
                     GYLWLYQAVPAFAGAVMGGFWQIFVMFGLHWGLIPLIINNFTVLGYDTMIPLLMPAIM
                     AQVGAALGVFLCERDAQKKVVAGSAALTGLFGITEPAVYGVNLPRKYPFVIACISGAL
                     GATIIGYAQTKVYSFGLPGIFTFMQTIPSTGIDFTVWASVIGGVIAIGCAFVGTVMFH
                     FITAKSQSAQVAQQEKTPEVITPEQGGICSPMTGEIVPLIHVADTTFASGLLGKGIAI
                     MPSVGEVRSPVAGRIASLFATLHAIGIESDDGVEILIHVGIDTVKLDGKFFSAHVNVG
                     DKVNTGDRLISFDIPAIREAGFDLTTPVLISNSDDFTDVLPHGTAQINAGEPLLSIIR
                     "
     gene            complement(16644..17480)
                     /gene="bglG"
                     /locus_tag="ACHKBL_002790"
     CDS             complement(16644..17480)
                     /gene="bglG"
                     /locus_tag="ACHKBL_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator BglG"
                     /protein_id="HFO5819642.1"
                     /translation="MNMQITKILNNNVVVVIDDQQREKVVMGRGIGFQKRPGERINSS
                     GIEKEYALSSHELNGRLSELLSHMPLEVMATCDRIISLAQERLGKLQDSIYISLTDHC
                     QFAIKRFQQNVLLPNPLLWDIQRLYPKEFQLGEEALTIIVKRLGVQLPKDEVGFIAMH
                     LVSAQMSGNMEDVAGVTQLMREMLQLIKFQFSLNYQEESLSYQRLVTHLKFLSWRIIE
                     HASINDSDESLQQAVKQNYPQAWQCAERIAIFIGLQYQRKISPAEIMFLAINIERVRK
                     EH"
BASE COUNT         4481 a         4431 c         4105 g         4599 t
ORIGIN      
        1 attgacaaca agattgtcga tcaccgccct tgatttgccc ttctgtagcc atcaccagag
       61 ccaaaccgat tagattcaat gtgatctatt tgtttgctat atcttaattt tgccttttac
      121 aaaggccatc tttcgtttat ttacttgttt tagtaaatga tggtgcttgc atatatatct
      181 ggcgaattaa tggatattgc aaatgtaata ttcacaggga tcactgtaat taaaataaat
      241 gaaggattat gtaatggaaa actttaaaca tctccctgaa ccgttccgca ttcgtgttat
      301 tgagccagta aaacgtacca ctcgcgctta tcgtgaagag gcaattatta aatccggtat
      361 gaacccgttc ctgctggata gcgaagatgt gtttatcgat ttactgaccg acagcggcac
      421 cggggcggtg acgcagagta tgcaggccgc gatgatgcgc ggcgacgaag cctacagcgg
      481 cagccgcagc tactatgcgt tagccgagtc agtaaaaaat atctttggtt atcaatatac
      541 tattccgact caccagggcc gtggtgcaga acaaatctat attccggtac tgattaaaaa
      601 acgcgagcag gaaaaaggcc tggatcgcag caaaatggtg gcgttctcta actatttctt
      661 tgataccacg cagggccata gccagattaa cggctgtacc gtgcgtaacg tctatatcaa
      721 agaagccttc gatactggcg tgcgttacga ctttaaaggt aactttgacc ttgagggatt
      781 agaacgcggc attgaagaag ttggcccgaa taacgtgcca tatatcgttg cgaccatcac
      841 cagtaactct gcaggtggtc agccggtttc actggcaaac ttaaaagcga tgtacagcat
      901 cgcgaagaaa tacgatattc cggtggtcat ggactccgca cgctttgctg aaaatgccta
      961 tttcatcaag cagcgtgaag cagaatacaa ggactggacc atcgagcaga tcacccgcga
     1021 aacctacaaa tatgccgata tgctggcgat gtccgccaag aaagatgcga tggtgccgat
     1081 gggcggcctg ctgtgcatga aagacgacag cttctttgat gtgtacaccg aatgcagaac
     1141 cctttgcgtg gtgcaggaag gcttcccgac atatggcggc ctggaaggcg gcgctatgga
     1201 gcgtctggcg gtaggtctgt atgacggcat gaatctggac tggctggctt atcgtatcgc
     1261 gcaggtgcag tatctggtcg atggtctgga agagattggc gttgtctgtc agcaggcggg
     1321 cggtcacgcg gcattcgttg atgcaggtaa actgttgccg catatcccgg cagatcagtt
     1381 cccggcacag gcgctggcct gcgagctgta taaagtcgcc ggtatccgtg cggtagaaat
     1441 tggctctttc ctgttaggcc gcgatccgaa aaccggtaaa caactgccat gcccggctga
     1501 actgctgcgt ttaaccattc cgcgcgcaac atatactcaa acacatatgg acttcattat
     1561 tgaagccttt aaacatgtga aagagaacgc ggcgaatatt aaagggttaa cctttaccta
     1621 cgaaccgaaa gtattgcgtc acttcaccgc aaaactgaaa gaagtttaat taaaacactc
     1681 agagtggcta taaggatgtt agccactcta ttaccctaca tcctcaataa caaaaatagc
     1741 cttcctctaa aggtggcatc atgactgatc aatcagaaaa aaaacactct gcattttggg
     1801 gtgttatggt tatagccggt acagtaattg gcggtgggat gtttgcttta cctgttgacc
     1861 ttgccggagc ctggtttttt tggggcgctt ttattcttat tatcgcctgg ttttcaatgt
     1921 tacattcagg gctattatta ttagaagcaa acttaaatta tcccgtcgga tccagtttta
     1981 acaccatcac caaagattta atcggtaaca cctggaacat tatcagcggt attaccgttg
     2041 ccttcgttct ctacatcctc acttatgcct atatctctgc taatggtgcg atcattagtg
     2101 aaacgatatc aatgaatttg ggttatcatg ctaatccacg tattgtcggg atctgcactg
     2161 ccattttcgt tgccagcgta ttgtggataa gttcgttagc cgccagtcgt attacctcat
     2221 tgttccttgg gctgaagatt atctcctttg taattgtttt tggttctttc ttcttccagg
     2281 tcgattactc cattctgcgc gatgccacca gcaccacggc gggaacatct tatttcccgt
     2341 atatctttat ggctttgccg gtgtgtctgg cgtcatttgg tttccacggc aatattccca
     2401 gcctgattat ttgctatggc aaacgcaaag ataagctaat caaaagcgtg gtcttcggtt
     2461 cgctgctggc gctggtgatt tatctcttct ggctctattg cacgatgggg aatatcccgc
     2521 gagaaagctt taaggcgata atctcctcag gcggcaacgt tgattcgctg gtgaaatcgt
     2581 tcctcggcac caaacagcat ggcattatcg agttttgcct gctggtgttc tccaacttag
     2641 ctgttgccag ttcgttcttt ggtgtcacgc tgggattgtt cgattatctg gcggatctgt
     2701 ttaagattga taactcccac gccgggcgtt tcaaaaccgt gctgttaacc ttcctgccac
     2761 ctgcgttgtt gtatctgatc ttcccgaacg gcttcattta cgggatcggc ggtgccggac
     2821 tgtgtgccac catctgggcg gtcattatcc ccgcggtgct ggcaatcaaa gctcgcaaga
     2881 agtttcccaa tcagatgttc acggtctggg gcggcaatct tattccggcg attgtcattc
     2941 tctttggtat aaccgtaatt ttatgctggt tcggtaacgt ctttaacgtg ttacctaaat
     3001 ttggctaaat ccttcaagaa gccagccatt cgctggcttc ttgcctctca ggaaatcact
     3061 tatgtccaaa tggcaactcg cctgatcctc cttcaccacg tatgctttgc gtcaccttac
     3121 tatcaggacg ctttagccca tgtcccgctt tttgatttgt agttttgccc tggttttact
     3181 ttatcccgca gggattgata tgtacctcgt tggtttaccg cgcatcgccg ccgatctcaa
     3241 tgccagcgaa gcgcagttgc atattgcgtt ctccgtttat ctggcgggga tggcggctgc
     3301 gatgttattt gccggtaaag tggccgatcg ttcagggaga aagcctgtcg ccatacccgg
     3361 cgcggcgcta tttattatcg cctcggtgtt ctgttcactg gctgaaacca gcgcgttatt
     3421 tcttgcaggg cgatttctac agggactggg cgcgggctgt tgttacgtag tggcgttcgc
     3481 cattttgcgc gacacgctgg atgatcgacg tcgggctaaa gtgctgtcgt tactcaacgg
     3541 cattacctgc atcattccgg tgttagcgcc ggtgctcgga cacctgatta tgcttaaatt
     3601 cccttggcag agtctgttct ggacgatggc aacgatgggc atcgcggtac tgatgttgtc
     3661 tttgtttatt ttaaaagaaa cgcgccctgc ggcccccaca acttcggaca aaccacgaga
     3721 aaatagcgag tcactgctta atcggttttt cctcagccgt gttgttatca ccaccctcag
     3781 cgtttcggtg atcctcacct tcgtcaacac gtcgccagta ttgctgatgg aaatcatggg
     3841 ttttgagcgc ggagaatacg ccaccattat ggcgttgacc gctggcgtca gcatgaccgt
     3901 ttcattctcc acgccgtttg cgctgggaat ttttaagcca cgtacgttga tgatcacctc
     3961 acaggtgtta ttcctggcgg cggggatcac ccttgccgtt tcaccttccc atgcggtttc
     4021 gctgtttggt atcacgctga tttgcgccgg tttctcggta ggttttggtg tggcgatgag
     4081 tcaggcgtta gggccgtttt cattacgcgc gggcgtagcc agctcgacct taggtattgc
     4141 gcaagtttgc ggttcgtcac tgtggatttg gctggcggcg gtggttggta tcggcgcatg
     4201 gaatatgctg atcgggattc tgattgcctg tagcatagtg agcctgttgc tgattatgtt
     4261 cgtcgcgcct ggacgcccgg ttgccgctca tgaagaaatc catcaccacg cttgatctca
     4321 atctgctgct ctgtctgcaa ctgctgatgc aggagcgcag cgtgaccaaa gcggcgaagc
     4381 ggatgaacgt gaccccttcg gcggtgagta agtcgctggc aaagttaaga gcgtggtttg
     4441 acgacccgct ctttgtgaac tcaccgctgg ggctgtcgcc cacaccgcta atggtcagca
     4501 tggagcaaaa tctggcggag tggatgcaaa tgagcaacct gctgctggat aaaccgcacc
     4561 accagacacc gcgcggcctg aagtttgagc tggcagcaga atcaccgcta atgatgatca
     4621 tgcttaatgc gctgtcgaaa cggatctacc aacgttaccc gcaggcgacc atcaaattac
     4681 gtaactggga ttacgattcc ttagatgcca ttactcgtgg tgaagtggat attggttttt
     4741 ccggtcgcga aagtcatccc cgttcgcggg agctgttaag ctcgctaccg ttagccattg
     4801 attatgaagt gctgtttagt gatgtgccct gcgtctggtt acgcaaagat catccggcac
     4861 tgcatgaagc atggaatctg gacacctttt tacgttatcc gcatatcagc atttgctggg
     4921 aacagagcga tacctgggcg ctggacaatg tgttacagga gctggggcgc gaacgcacga
     4981 ttgctatgag cctgccggaa ttcgagcagt cactgtttat ggcagcacag cccgacaatc
     5041 tgctactggc gaccgcaccg cgctactgtc agtactacaa tcaactccac caactgccgt
     5101 tggttgctct tcctctcccg tttgacgaaa gccagcaaaa aaagctggaa gtccctttta
     5161 ccctgctgtg gcataaacgg aacagccata atccgaagat cgtctggtta cgggaaacca
     5221 ttaaaaatct ttacgcgtcg atggcataac cgaatcgtat gaaacgcgac ccaatttcac
     5281 aataaaatgt aaaaaagttg taataagctt gtctgaatcg aacttttagc cgctttagtc
     5341 tgtccatcat tccagtaaat gattactctt gtattcataa tggaccatta agcatggagc
     5401 gaaaaatggc gactcacttt gcccgaggga ttttaacgga aggacatctg atttctgttc
     5461 gtctcccctc ccagtgtcat caagaagccc gaaacattcc ccctcatcgt caaagccgtt
     5521 ttctggcgtc cagaggttta ctcgcagaac tgatgttcat gctgtatggc attggcgaat
     5581 tgccggaaat cgtcaccctg ccgaaaggaa aaccggtttt cagcgataaa aatttgcctt
     5641 cgttttctat ttcctatgcc gggaatatgg tcggcgtggc gttaacaacc gaaggtgaat
     5701 gtggcctcga tatggaacta cagcgtgcga cgcgcgggtt tcatagccca cacgcgcccg
     5761 ataaccacac cttttccagc aatgaatcgc tatggatcag taaacaaaac gatcctaacg
     5821 aagcgcgggc gcagctcatc acgctgcgcc gaagcgtgct aaaactaacc ggtgatgttt
     5881 tgaatgacga tccgcgcgat ctgcaactac tgcccattgc cggacgcctg aaatgtgctc
     5941 atgtaaatca tgtagaagcg ttatgcgacg cggaggacgt gctggtgtgg tccgtggcgg
     6001 tcacgcccac gattgaaaag ctcagtgtct gggagttaga tggcaaacat ggctggaaaa
     6061 gcctgcccga tattcacagc cgcgccaaca atcctaccag ccggatgatg cgttttgccc
     6121 agctctctac cgtgaaggct ttttcgccaa attgatagac aaccacagga gtcatcatgt
     6181 ctgaaaaatt gcaggtggtt acgttactgg ggagcctgcg caaaggctca tttaatggca
     6241 tggttgcacg taccctgccg aaaattgctc cggcgagcat ggaaatcaat gcgttacctt
     6301 ccattgccga cattcccctg tatgacgctg acgtacagca ggaagacggt tttccagcaa
     6361 cagttgaagc tctggcggaa cagatccgtc aggctgacgg tgtggtgatc gtcacgccgg
     6421 aatataacta ctcggtaccg ggtgggctga aaaatgccat cgactggctt tcccgcctgc
     6481 cggatcaacc gctggctggt aaaccggtat tgattcagac cagctcaatg ggcgtgattg
     6541 gcggcgcgcg ctgtcagtat cacctgcgcc agattctggt tttcctcgat gcaatggtga
     6601 tgaacaagcc ggaatttatg ggcggcgtga ttcagaacaa agttgatccg caaaccgggg
     6661 aagtgattga tcagggtacg ctggaccacc tgaccgggca attgaccgca tttggtgagt
     6721 ttattcagcg agttaagatc taaataaaaa acccgccagc aatcatgcat ggcgggtttt
     6781 taacgcgcta tcgattttag tgagcgtcga taaagacaat cttcaggata aacagcagcg
     6841 caacgatgat tacgcacggg ctaaggtcac gcagacgtcc ggtaccaatc ttcatcacgc
     6901 agtaggagat aaagcccagc gcgatacctt cagtaatcga gaagctgaac ggcatcatca
     6961 cagcggtaat aaacgccgga acagattcag taagatcctg ccagttcacg cgagcaagac
     7021 ttgaggtcat caacacgcca acgtaaatca gcgcaccagc tgcagcgtag cctggcacca
     7081 tccccgccag cggcgacagg aagataacca gcaggaacag cagaccaaca accactgccg
     7141 tcagaccggt acgaccgcca acggaaacgc cggaagaaga ttcaatatac gccgtaacag
     7201 aagaagtacc gataaacgaa ccggtcaccg aagagatact gtcgacatac agcgcctgct
     7261 tcatgcgcgg gaatttacct ttctcatcag ccagacctgc tttatcggtc acgccaatca
     7321 gcgtaccgga ggagtcaaac aagttgacca gcatgaaaga gaaaatcacc cctgccagcc
     7381 caaggttaaa cgacccggct aaatctacat gacccacgac actcattacg ctcggcggcg
     7441 cagaaacgat gccattatag tggacatcac ccagcatcca gcccagcagc gttgtcacca
     7501 cgatggaaac cagcaccgcc gcgtgaatat tgcgcgaggc cagaatggcg atgatgaaga
     7561 agccgaggat ccccagcagt acgctgtgag aagtcagatt accgatgctc accagcgttt
     7621 ccgggttagc gacaatcaca cctgcattct tcagtcccat catgccaata aacaggccga
     7681 taccgctggt aatgcccaca cgcagactca ccggaatgtt ggctatcatc cagtagcgaa
     7741 cgcggaaaat cgtcagtaac agcagaccaa tcgcgcccca gaagattgcg cccatcccga
     7801 cctgccacgg caagcccatc gcctgcacaa ccacaaaagc gaagaacgca ttcaggccca
     7861 tagcgggtgc cagtgcaact ggcaggttag caaacagtcc catcataata ctgccgaatg
     7921 cagcaatcag acaggtagta acgaagacgg cgctggtatc catgccagca acgccaagaa
     7981 tttgcgggtt aacaaaaacg atgtaaacca tcgtcaggaa ggtggtaaaa ccggcgatca
     8041 cttcggtccg tgccgtcgtg ccatgttcgc gcagtttaaa cacgcgttcc agcatcccct
     8101 ggccagaagc ctgggttgtg tgttgttgac tcattatcta tttccgaaca aggagggaaa
     8161 atccgtcgct atcgtatacc aaaatgagac aatatggacg gctgaagata actttttttc
     8221 attgtatttg cttatacggc aacgattgcg tgatgcgaat tggcatgacg aaaacgttaa
     8281 actaacgata aaggaaagac atgtcccaga tagaagcggt atttttcgac tgcgacggta
     8341 cgctggtcga tagcgaggtc atttgctctc gcgcctatgt cacgatgttc agggaatttg
     8401 gcattcacgt tgacctggaa gagattttta cccgttttaa aggcgtaaaa ctctacgaaa
     8461 tcatcgacat catcagtaaa gaacagggcg tgacgatggt ccaggccgat gccgagcacg
     8521 tttaccgcgc agaagtagca cgtctgtttg ataccgaact tgaggacatc gcaggagcga
     8581 acgaattgct ggcgagcatt aacgtgccga tgtgcgtcgt ctctaacggt ccggtcagca
     8641 aaatgcagca ttcgcttggt aagctcaaca tgctgcacta cttcccggaa aaactgttca
     8701 gcggctacga tatccagcgt tggaagcccg acccggcgtt aatgttccat gcggcaaaag
     8761 cgatgaatgt gaacgttgaa aactgcattc tggtcgatga ctccagcgcc ggtgcgcaat
     8821 caggaatcga tgctggtatg gaagtgtttt acttctgcgc tgacccgcac aataagccga
     8881 tcgttcatcc gaaagtcacc acctttaccc atctttcgca attaccggaa ctgtggaagg
     8941 cgcgtggttg ggatattacg gcatagttat tcacactccc ttcacttact ccacttaaat
     9001 tggcacgcaa aggtaagtaa agggagtttg atatgtctgt ttcacgtcgg gtaattcatc
     9061 acggactgta ttttgcggtt ttagggccgt taattggtgt tctgtttctt gtcctctaca
     9121 tattcttcgc aaaagaaccg ctgattcttt tggtaataat acaggtactt ccattgtttc
     9181 tcttaatgtc gataactacg ggagctattc ctgcaatgtt aactggcgta atggtcgcct
     9241 gtctgccgga aaagatcggg tcacagaaac gttatcgttg tctggttggc ggcataggtg
     9301 gagtcgttat caccgagatc tattgcgctg ttatcgtaca tattaaggac atggcttcct
     9361 cagcgttgtt tgaaaacatt ctttctggtg aaaatctcgt tgtccgcatc atcccagcat
     9421 tgctggcagg cgtagtgatg agcagaatca ttacacatct acccggattg gatatttcat
     9481 gtcctgaaac agactcttta agctaagcgg aatgctttat ctatttgtca ttaaatgttg
     9541 gagaacaggc cggagcgtaa ttcacacatc cggccttatt tttaagctta accgagcgct
     9601 aattctgcgg cagcggcttt atcagcgatg actatcagcg aagggtgcaa ctgcaatacg
     9661 gaagcaggaa catcttcggt aaccggacct tgcagaacat ttttcagtgc ctgcgcttta
     9721 cccgcgccac tgacaattat cagcaagttt ttcgccgcca tgatgctttt cggccccatc
     9781 gtcacgtagc tgtcaggcac taatgaaaag tcgccaccca actcgccatg cgccacaata
     9841 tcaaccatct ctccctggat cgggaactcc acggtttgct catggaaatg ggtcgtattt
     9901 ggcagattgc cacaaaaatg accatctaca cctaatccca acaccaccaa atccaggccg
     9961 ccttcacgcg ccagtttctg atcatgctcg cggtagttat caatagtgag cttctggata
    10021 ttctcttctt tgatccctgc aggggtgaaa aacagattat gcagattggt aatcgttacg
    10081 ccttcccctt ctttaccgcg aaatggaatt tcatcgaaat tgtagaaata gcagttatcg
    10141 taccagggct tacctttaac cagagtgatg agatattcgt acatgccttt gggcgtgctc
    10201 ccggcggtaa ttgccaggtt aacgcgacgc atctttgaca tataaccaag cagatgatgt
    10261 gccgcgacac ggctcatttc ctggtaatct tcggtaatga tcaatttcat cttaatgcct
    10321 tatattattt gaataatttc tgtacaaagg ctgcataggc tggtcgccag acatccattt
    10381 catggttcag accgggatat tcctggtaat caaacttaat ttttttctgc tcaagctcag
    10441 ttttcagccc ggcgatatcc ttgccggtta cgacatcttt atcgcccacg accacagtaa
    10501 aattacgtag ttgctggtta atagctgccg gctcgttcag ccgggcagcg accccttcat
    10561 ctggtacggt cgtcgtggta acaccactga atgtggccag ccagccaaag ctttccagat
    10621 gattcattcc cgataccagt gcctgatacc caccttgtga aagccctgcc agtgcgcgac
    10681 catcggcatc tttacggaca ttaaaacgct tgctaatcag cgggataata tcgttcatca
    10741 attcgcgatc tgccgcttta gcatttaacg gataaaagac tttacgtctc tcctgaggaa
    10801 cgaaatcttc gggaataatg cccttcgcat cggtttcggt atccgggatc accaccagca
    10861 tcggtttaat tttcccttca gccagcaggt tatccatgat ttgcgggata cgcccctgat
    10921 caatagcgga acgtccggta tcaccaaagc cgtgatagaa atagagcact ggcaaaggct
    10981 cgcctatgcc ggtgtatcct ggcggggtcc agacatacat ctgacgttcg gattgcaatg
    11041 cgttggagtg gtaagttatg gcgatcaaat caccatgcac aacagaacgt gtatccagat
    11101 aactgcccgg taccagaatc atactggagt taacctggcg ctgaggctta gtcattgctg
    11161 tacctgtatc aatgctgcgt acaccatcaa cattgaaaaa atactcgtac aggttgcctt
    11221 tcagagcagg cgtgcgccac gaccagactc ccgcttcgtc tttggtcatc gggtgaatat
    11281 tgtccggaac cggaacaccc accactacag agacattttt tgccccaggg gcaaagtagc
    11341 gaaaggtcac gctgttatcg gtattgacct gagtcacata ctgtttcacc ggaatagtgg
    11401 gtgccgggct ggctggcata tccgctgcta tggcccattg agcagaaata ccgctggcaa
    11461 tagccagcgt taaagcagca atttttatat tcatcgtaat attcctttta ttaagttcca
    11521 ttaccaccag atttcagcct gggcaccgac agcaaactgg tcgtctttat tgtcttcaaa
    11581 ggtgaagcca tccagttcgt tttccagagc tttgatatat gtagcgtaga aacgaatttc
    11641 cgggcgcgag gtcaacatac tggtattgac tttaaaggta tggaagagcg tggttttata
    11701 accagactca ttatatttat tgctattcgc atctttgttt tgctgggtga aatagcccag
    11761 ttcaacacct gtctggttat attggtccca aatataggcc gggcgaacaa ccgcacgaat
    11821 agattcgaaa tcagaatgtg cgcctgtttc gtagctataa atatcgttac cgaaggagta
    11881 aacaatcgcg ttagccacaa tgaaatgatc gccgatataa gcttcgccct gcgaagtcag
    11941 acgaactgca gttccgcctg tgtgatcacc ataataacga ccattaaagg tagtaaatgg
    12001 acttgcacca gcataacgac caaagttact ggcgatagag ttattcgcaa ccaggaagga
    12061 gaattcgttg aagccaccct tatcaaattt ctgcgttaaa gatgtaccaa acatccaggt
    12121 atctttccag tcataatacc cgttattatc ctgattatct ttttccgatg cgctttcatt
    12181 tgctgagaca taacgaccgc tcaccattaa ggtggcttta tcccataacg ggatatcttt
    12241 atagcgtaaa tcaatggtat tggtattaat ctgctgtttg ttttgcaggc tgcgatcgta
    12301 atcatcgaca tcttcacgaa ccagcgcgat atcaatttta cccggaccga ctttccagtt
    12361 ttccagacct acacctgcgg cagcatcggt acgctgcgtt ttccagtcaa gcatctggat
    12421 ttcaattttc ggcgcaccgt gtttacccac ccagaaatca gcctctggcg caaagggcag
    12481 gaaacctttg gtggtaacgt acatatcaga gaactgcata aagttctcac caccggcgtt
    12541 atcgccaaac cagccagtag agtactgctg accaacgtta ccatccatca tcacgatggc
    12601 atcaacccgt ttgccgtttt cgttgtagac acgttgtttt aactgcaaat caaaccagcc
    12661 ggagtattcg ttaccaaagc ggcccagaga accaattgcc catgatttag gtgaaccatg
    12721 agaggctgtt ccccaaccag aacggtagta gccgttgtaa ctaaagccga tttcatcttt
    12781 cacaaattta ctgaaatctt tcagagtcat ggaagaataa atcgagcctg tctcactggc
    12841 atttttctct tcatttttaa ccagtacagc atcaggcttt gcggcacgac tggtcgggaa
    12901 tggattggcg gcataccctt gatcattcgt acttacgcta cgattcaccg tcgctggcgt
    12961 atactttttc ttctcttcat ctttatattt tttcagttcg ctttgcgttt ctcttaacgc
    13021 cttttctaat agctcaaggc gttgttccac tgttaatgat tcagccaatg attgtgcgga
    13081 aaaggctaac ggtgccatta ataagatggc agaggtaata agatttcgtc taaacataat
    13141 atccctttat ggtgcaaaga aagaattaac gcatcgcatc cagactgttc tgaatgcgac
    13201 gataattaag gtgctttaat ggttattttt ttaatgacag cccccgagtt ttgatcactt
    13261 ctgcatacca tccgaagctt ttcttgcgcg ttcttgtgag gcttccttca ccattatcat
    13321 cacgatccac ataaataaag ccgtagcgct tagacatttg tgaatgagat gcactgacta
    13381 aatcaattgg cccccaactg gtgtacccca taatatccac accatcggca atcgcttcat
    13441 ttacctgtac caggtgatcg tttaaatagg caattcgata atcgtcctgt atcgaaccat
    13501 ccacttcaac gctgtctttt gcgcctaatc cgttctcgac aataaataac ggtttttgat
    13561 aacgatccca aagcgtattt aacagaatcc gtaatccaac cggatcaatt tgccaccccc
    13621 actctgaact tttcagatgc ggatttggga tcatattcag tatgttgccc tgcgcatttt
    13681 tattaatgct ttcgtcgtgg gaaacacaac cggtcatgta ataactaaaa gagatgaaat
    13741 ctacggtatg ttttaaatct tctgcgtcac tttcagtcat ctcaatggtg atattgtggt
    13801 cgcggaagaa acgctgcata tagccgggat actgaccacg cgcctgaaca tcaccaaaga
    13861 acatccagcg ccggttctct tccatggcct gcaacatatc ctgtggctgg caggtgaggg
    13921 ggtaaaccag cccaccgaga agcatattgc caattttcgc ttcggggatc aggctatgac
    13981 aggctttaac tgcccgcgca ctggcaacca gttgatgatg gatcgcctga taaacttccg
    14041 cctcgccact ctcttctgcc agccccactc ccgtgaatgg cgcgtgtaat gacatgttga
    14101 tttcattaaa tgtcagccat aacacaactt tatgttggta gcgggtgaag accgtgcggg
    14161 cgtaatgctc gaagtgatcg atgaccgccc gattagccca accgccgtag tttttcacca
    14221 gcccatatgg catttcgtaa tgggataacg ttaccagcgg cttgatcccc gcctgcgcca
    14281 tttcatcaaa cagccgatcg taaaacgcta accccgcttc attcggttcg gcttcgtcgc
    14341 cctgagggaa aattcgcgcc caggcaatgg aaatacgcag acaggtgaag cccatctcgg
    14401 caaataacgc gatatcttcc gggtaacggt gataaaaatc gatggcgaca tctttgatat
    14461 tctctttccc caggatgcgc ggttccattt ttcccattac gccatgaggc tgtaaatctg
    14521 aggtcgtgat ccctttgcca tcttcctgcc aggcgccttc cacctgattg gcagctgttg
    14581 caccacccca aagaaatgtt tctggaaatg ctttcataat taactccttt tatcgttagc
    14641 gaatgatgga taacagcggc tcaccagcgt ttatctgcgc cgtgccgtgg ggtaatacgt
    14701 ccgtaaaatc atcgctatta ctgattaata ccggcgtcgt cagatcaaat ccggcctcgc
    14761 gaatagcagg gatatcaaaa gaaatcagcc gatcgcctgt attgaccttg tcacccacgt
    14821 tgacgtgagc ggaaaagaat ttgccgtcca gttttacggt atcgataccg acatgaatca
    14881 ggatctccac accatcatct gactcaatgc caatggcgtg taatgtggcg aacaacgaag
    14941 caattcgacc tgccaccgga gaacgcactt caccaaccga gggcataatg gcaatacctt
    15001 tacccaacag gccgctggca aacgtggtat cagcgacgtg aatgagcggc acaatctctc
    15061 ccgtcatcgg tgaacagata ccgccctgct caggtgtaat aacctctggt gttttctctt
    15121 gctgggcaac ttgcgctgac tgactcttag cggtgatgaa atgaaacatc accgtaccga
    15181 caaatgcgca accgatggca atgacaccgc caataacgct ggcccatacg gtgaaatcaa
    15241 tccccgttga cgggatggtt tgcatgaagg tgaaaatacc tggcaaacca aaggagtaaa
    15301 ctttcgtttg cgcgtagcca atgatggtgg cccccaatgc cccacttatg caggcgataa
    15361 cgaaggggta cttacgcggc agattgacgc catataccgc tggttcggtg ataccaaaca
    15421 aacctgtcaa cgccgctgat cccgccacca cttttttctg tgcatcgcgt tcgcagagga
    15481 agacgccgag cgccgccccg acttgcgcca taatggcggg cattaacagc gggatcatgg
    15541 tgtcgtagcc cagcacggtg aagttattga taattaaagg tatcaaaccc caatgcagac
    15601 cgaacatcac aaagatttgc cagaagccgc ccattaccgc gcctgcaaat gcaggaaccg
    15661 cctgataaag ccagagataa ccggcagcaa tcagttcact tatccaggtt gatagcggcc
    15721 ccaccagcag aaatgtgatg ggcgtgatga ccatcagaca tagcaagggt gtgaagaaat
    15781 ttttgattgc cgaaggtaac cacgcattaa gtcggcgttc cagaatgctg cacaaccagg
    15841 cagaaaaaat aatgggaata accgatgacg agtaattcaa caatgtgacc ggaataccca
    15901 ggaaatccag gcccagcgca tccacttttt gcccgttctc gaaggcagtc agaattaatg
    15961 gatgcactaa cgctccgcca atcaccatgg cagtaaatgg attgccgccg aagcgtttcc
    16021 ctgcggtgta gcccaggatt atcgggaaga accaaaacaa ggcatcactg gcgctaaata
    16081 aaattaaata agtaccactt tgttcggtcg tccactgaaa agtgagtgcc agagccaaca
    16141 tacctttcag gatcccggtt gccgccatca aaccgatcag aggcgtaaaa atacctgaaa
    16201 taacataaac aaagcggttt aacagattac ctttatcatc attttccggt gcctgttgcg
    16261 ctttttcgcc aaggcctgcc acgctgttaa ccgccaggaa gacatcggcc acatggttac
    16321 ctatgacaac ctgaaactgg ccaccgcttt ccaccaccat aataataccg ggggtctttt
    16381 tcagtacctc agcttgcgct ttgctttcat cctttaactt aaaacgtaat cgcgttgcac
    16441 aatgcatcag actcacaatg ttatctgcgc ccccgactcc tgcgactatt gttctggcta
    16501 actccgtcat aacttgccct ctaccgcttt gcggcaaaac tccaaaaaaa aacctgaaaa
    16561 aaacggcctg acgtgaatca agcaattttt ttcaggtttt gcccgcttag tgcggtaaca
    16621 atcctttatt cagtaataat atttcagtgt tctttgcgca cgcgctctat atttatggct
    16681 aaaaacataa tctctgcagg tgagatttta cgttgatact gcaaaccaat aaaaatggcg
    16741 atccgctccg cacattgcca tgcttgcggg taattttgct ttactgcttg ttgtaatgat
    16801 tcatcactat cgttaatcga tgcatgttca ataatacgcc aggataaaaa tttcagatgc
    16861 gtaaccagtc gctgataact caagctttct tcctggtaat taaggctgaa ctgaaatttt
    16921 attaattgca gcatttctcg cattaattgc gtgacacctg caacatcctc catatttccg
    16981 ctcatttggg cactgaccag atgcatggca ataaagccca cttcatcttt cggtaactgc
    17041 acgcccaacc gtttaacaat aatggttaac gcttcttccc ctagctggaa ctctttcggg
    17101 taaagtcgct ggatatccca cagcagcggg ttgggcagta gcacgttttg ctgaaagcgt
    17161 ttaatcgcaa actggcaatg gtcagttagc gagatataaa tactgtcctg caactttccc
    17221 aggcgctcct gcgccagaga gataatacga tcacaggttg ccatcacctc aagaggcata
    17281 tgacttaaga gttcgcttaa tcgcccgttc agttcatgac tgctcaaggc atactctttt
    17341 tctattccac ttgagttaat tctttcgcct gggcgttttt gaaagccaat tccgcgcccc
    17401 atgacgactt tttcccgctg ttgatcatca ataaccacca caacattatt gttgagaatt
    17461 ttggtgattt gcatgttcat agcaaggacc ttttttataa acaaaaaaac ccgacttcac
    17521 cagtattctc tggttatgtc aggttttgcc tgcgaatgca gtaacaatcc agtcattaat
    17581 taatggtttt tataacgaac atccaggttc gaaaat
//
LOCUS       DAZFLD010000100        17556 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.101, whole genome shotgun sequence.
ACCESSION   DAZFLD010000100 DAZFLD010000000
VERSION     DAZFLD010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17556
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.101"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            76..1125
                     /gene="allD"
                     /locus_tag="ACHKBL_002791"
     CDS             76..1125
                     /gene="allD"
                     /locus_tag="ACHKBL_002791"
                     /EC_number="1.1.1.154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate dehydrogenase"
                     /protein_id="HFO5819643.1"
                     /translation="MKISRETLHQLIENKLCQAGLKREHAATVAEVLVYADARGIHSH
                     GAVRVEYYAERISKGGTNREPEFRLEETGPCSAILHADNAAGQVAAKMGMEHAIKTAQ
                     QNGVAVVGISRMGHSGAISYFVQQAARAGLIGISMCQSDPMVVPFGGAEIYYGTNPLA
                     FAAPGEGDEILTFDMATTVQAWGKVLDARSRNMSIPDTWAVDKNGAPTTDPFAVNALL
                     PAAGPKGYGLMMMIDVLSGVLLGLPFGRQVSSMYDDLHAGRNLGQLHIVINPNFFSSS
                     ELFRQHLSQTMRELNAITPAPGFNQVYYPGQDQDIKQRKAAVEGIEIVDDIYQYLISD
                     ALYNTSYETKNPFAQ"
     gene            1147..2382
                     /gene="allC"
                     /locus_tag="ACHKBL_002792"
     CDS             1147..2382
                     /gene="allC"
                     /locus_tag="ACHKBL_002792"
                     /EC_number="3.5.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047652 - allantoate deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA];
                     GO_process: GO:0009442 - allantoin assimilation pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoate deiminase"
                     /protein_id="HFO5819644.1"
                     /translation="MITHFRQAIEETLPWLSSFGADPAGGMTRLLYSPEWLETQQQFK
                     KRMAASGLETRFDEVGNLYGRLSGTEYPQEVVLSGSHIDTVVNGGNLDGQFGALAAWL
                     AIDWLKTQYGAPLRTVEVVAMAEEEGSRFPYVFWGSKNIFGLANPDDVRNICDAKGNS
                     FVDAMKACGFTLPNAPLTPRQDIKAFVELHIEQGCVLESNGQSIGVVNAIVGQRRYTV
                     TLNGESNHAGTTPMGYRRDTVYAFSRICHQSIEKAKKMGDPLVLTFGKVEPRPNTVNV
                     VPGKTTFTIDCRHTDAAVLRDFTQQLENDMRAICDEMDIGIDIDLWMDEEPVPMNKEL
                     VATLTELCESEKQNYRVMHSGAGHDAQIFAPRVPTCMIFIPSINGISHNPAERTNITD
                     LAEGVKTLALMLYQLAWQK"
     gene            2393..3178
                     /gene="allE"
                     /locus_tag="ACHKBL_002793"
     CDS             2393..3178
                     /gene="allE"
                     /locus_tag="ACHKBL_002793"
                     /EC_number="3.5.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071522 - ureidoglycine aminohydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0010136 - ureide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-ureidoglycine aminohydrolase"
                     /protein_id="HFO5819645.1"
                     /translation="MGYLNNVTGYRDDLLANRAIVKHGNFALLTPDGLVKNIIPGFEN
                     CDATILSTPKLGASFVDYLVTLHQNGGNQQGFGGEGIETFLYVISGNITAKAEGKTFA
                     LSEGGYLYCPPGSLMTFVNAQAEDSQIFLYKRRYIPVEGHAPWLVSGNASELEHIHYE
                     GMDDVILLDFLPKELGFDMNMHILSFAPGASHGYIETHVQEHGAYILSGQGVYNLDNN
                     WIPVKKGDYIFMGAYSLQAGYGVGRGEAFSYIYSKDCNRDVEI"
     gene            complement(3307..4452)
                     /gene="glxK"
                     /locus_tag="ACHKBL_002794"
     CDS             complement(3307..4452)
                     /gene="glxK"
                     /locus_tag="ACHKBL_002794"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 3-kinase"
                     /protein_id="HFO5819646.1"
                     /translation="MKIVIAPDSFKESLSAEKCCQAIKAGFSTLFPDAHYICLPIADG
                     GEGTVEAMVAATDGNIVKLEVCGPMGEKVNAFYGITGDGKTAVIEMAAASGLMLVAPE
                     KRNPLLASSFGTGELIRHALDNGIRHIILGIGGSATVDGGMGMAQALGVRFLDAYGQV
                     LAANGGNLARVASIEMDECDPRLANCHIEVACDVDNPLVGARGAAAVFGPQKGATPEM
                     VEELEQGLQNYARVLQQQTEINVCQMAGGGAAGGMGIAAAVFLNADIKPGIEIVLNAV
                     NLAQAVQGAALVITGEGRIDSQTAGGKAPLGVASVAKQFNVPVIGIAGVLGDGVEVVH
                     QYGIDAVFSILPRLAPLAEVLASGETNLFNSARNIACAIKIGQGIKN"
     gene            complement(4474..5775)
                     /gene="ybbY"
                     /locus_tag="ACHKBL_002795"
     CDS             complement(4474..5775)
                     /gene="ybbY"
                     /locus_tag="ACHKBL_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil/xanthine transporter"
                     /protein_id="HFO5819647.1"
                     /translation="MFNFAVSRESLLSGFQWFFFIFCNTVVVPPTLLSAFQLPQSSLL
                     TLTQYAFLATALACFAQAFCGHRRAIMEGPGGLWWGTILTITLGEASRGTPINDIATS
                     LAVGIALSGVLTMLIGFSGLGHRLARLFTPSVMVLFMLMLGAQLTTIFFKGMLGLPFG
                     IADPNFKIQLPPFALSVAVMCLVLAMIIFLPQRFARYGLLVGTITGWLLWYFCFPSSH
                     SLSGELHWQWFPLGSGGALSPGIILTAVITGLVNISNTYGAIRGTDVFYPQQGAGNTR
                     YRRSFVATGFMTLITVPLAVIPFSPFVSSIGLLTQTSDYTRRSFIYGSVICLLVALVP
                     ALTRLFCSIPLPVSSAVMLVSYLPLLFSALVFSQQITFTARNIYRLALPLFVGIFLMA
                     LPPVYLQDLPLTLRPLLSNGLLVGILLAVLMDNLIPWERIE"
     gene            complement(5832..7193)
                     /gene="allB"
                     /locus_tag="ACHKBL_002796"
     CDS             complement(5832..7193)
                     /gene="allB"
                     /locus_tag="ACHKBL_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004038 - allantoinase activity [Evidence
                     IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase AllB"
                     /protein_id="HFO5819648.1"
                     /translation="MSFDLIIKNGTVILENEARVVDIAVKGGKIAAIGQDLGDAKEVM
                     DASGLVVSPGMVDAHTHISEPGRSHWEGYETGTRAAAKGGITTMIEMPLNQLPATVDR
                     ASIELKFDAAKGKLTIDAAQLGGLVSYNIDRLHELDEVGVVGFKCFVATCGDRGIDND
                     FRDVNDWQFFKGAQKLGELGQPVLVHCENALICDELGEEAKREGRVTAHDYVASRPVF
                     TEVEAIRRVLYLAKVAGCRLHVCHISSPEGVEEVTRARQEGQDVTCESCPHYFVLDTD
                     QFEEIGTLAKCSPPIRDLENQKGMWEKLFNGEIDCLVSDHSPCPPEMKAGNIMKAWGG
                     IAGLQSCMDVMFDEAVQKRGMSLPMFGKLMATNAADIFGLQQKGRIAPGKDADFVFIQ
                     PNSSYVLTNDDLEYRHKVSPYVGRTIGARITKTILRGDVIYDIEQGFPVAPKGQFILK
                     HQQ"
     gene            complement(7254..8708)
                     /gene="ybbW"
                     /locus_tag="ACHKBL_002797"
     CDS             complement(7254..8708)
                     /gene="ybbW"
                     /locus_tag="ACHKBL_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative allantoin permease"
                     /protein_id="HFO5819649.1"
                     /translation="MEHQRKLFQQRGYSEDLLPKTQSQRTWKTFNYFTLWMGSVHNVP
                     NYVMVGGFFILGLSTFSIMLAIILSAFFIAAVMVLNGAAGSKYGVPFAMILRASYGVR
                     GALFPGLLRGGIAAIMWFGLQCYAGSLACLILIGKIWPGFLTLGGDFTLLGLSLPGLI
                     TFLLFWLVNVGIGFGGGKVLNKFTAILNPCIYIVFGGMAIWAISLVGIGPIFDYIPGG
                     IQKAENSGFLFLVVINAVVAVWAAPAVSASDFTQNAHSFREQALGQTLGLVVAYILFA
                     VAGVCIIAGASIHYGADTWNVLDIVQRWDSLFASFFAVLVILMTTISTNATGNIIPAG
                     YQIAAIAPTKLTYKNGVLIASIISLVICPWKLMENQDSIYLFLDIIGGMLGPVIGVMM
                     AHYFMVMRGQINLDELYTAAGDFKYYDNGFNLTAFSVTLVAVILSLGGKFIPFMEPLS
                     RVSWFVGVIVAFAAYALLKKRTAAEKTGEQKVTG"
     gene            complement(8878..9756)
                     /gene="glxR"
                     /locus_tag="ACHKBL_002798"
     CDS             complement(8878..9756)
                     /gene="glxR"
                     /locus_tag="ACHKBL_002798"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008679 - 2-hydroxy-3-oxopropionate
                     reductase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046487 - glyoxylate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HFO5819650.1"
                     /translation="MKLGFIGLGIMGTPMAINLARAGHQLHVTTIGPVADELLSLGAV
                     SVETARQVTDASDIIFIMVPDTPQVEDVLFGENGCTKASLKGKTIVDMSSISPIETKR
                     FARQVNELGGDYLDAPVSGGEIGAREGTLSIMVGGDEAVFERVKPLFELLGKNITLVG
                     GNGDGQTCKVANQIIVALNIEAVSEALLFASKAGADPVRVRQALMGGFASSRILEVHG
                     ERMIKRTFNPGFKIALHQKDLNLALQSAKALALNLPNTATCQELFNTCAANGGSQLDH
                     SALVQALELMANHKLA"
     gene            complement(9856..10632)
                     /gene="hyi"
                     /locus_tag="ACHKBL_002799"
     CDS             complement(9856..10632)
                     /gene="hyi"
                     /locus_tag="ACHKBL_002799"
                     /EC_number="5.3.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxypyruvate isomerase"
                     /protein_id="HFO5819651.1"
                     /translation="MLRFSANLSMLFGEYDFLARFEKAAQCGFRGVEFMFPYDYDIEE
                     LKQVLASNKLEHTLHNLPAGDWAAGERGIACIPGREEEFRDGVAAAIRYARALGNKKI
                     NCLVGKTPTGFSSEQIHTTLVENLRYAANMLMKEDILLLIEPINHFDIPGFHFTGTRQ
                     ALKLIDDVGCCNLKIQYDIYHMQRMEGELTNTMTQWADKIGHLQIADNPHRGEPGTGE
                     INYEYLFTVIENSDYNGWVGCEYKPQTTTEAGLRWMDPYR"
     gene            complement(10645..12426)
                     /gene="gcl"
                     /locus_tag="ACHKBL_002800"
     CDS             complement(10645..12426)
                     /gene="gcl"
                     /locus_tag="ACHKBL_002800"
                     /EC_number="4.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009028 - tartronate-semialdehyde synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate carboligase"
                     /protein_id="HFO5819652.1"
                     /translation="MAKMRAVDAAMYVLEKEGITTAFGVPGAAINPFYSAMRKHGGIR
                     HILARHVEGASHMAEGYTRATAGNIGVCLGTSGPAGTDMITALYSASADSIPILCITG
                     QAPRARLHKEDFQAVDIEAIAKPVSKMSVTVREAALVPRVLQQAFHLMRSGRPGPVLV
                     DLPFDVQVAEIEFDPDMYEPLPVYKPAASRMQIEKAVEMLIQAERPVIVAGGGVINAD
                     AAALLQQFAELTSIPVIPTLMGWGCIPDDHELMAGMVGLQTAHRYGNATLLASDMVFG
                     IGNRFANRHTGSVEKYTEGRKIVHIDIEPTQIGRVLCPDLGIVSDAKAALTLLVEVAQ
                     EMQKAGRLPCRKEWVADCQQRKRTLLRKTHFDNVPVKPQRVYEEMNKAFGRDVCYVTT
                     IGLSQIAAAQMLHVFKDRHWINCGQAGPLGWTIPAALGVCAADPERNVVAISGDFDFQ
                     FLIEELAVGAQFNIPYIHVLVNNAYLGLIRQSQRAFDMDYCVQLAFENINSSEVNGYG
                     VDHVKVAEGLGCKAIRVFKPEDIAPAFEQAKILMAQYRVPVVVEVILERVTNISMGSE
                     LDNVMEFEDIADNAADAPTEACFMHYE"
     gene            complement(12516..13331)
                     /gene="allR"
                     /locus_tag="ACHKBL_002801"
     CDS             complement(12516..13331)
                     /gene="allR"
                     /locus_tag="ACHKBL_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor AllR"
                     /protein_id="HFO5819653.1"
                     /translation="MTEVRRRGRPGQAEPVAQKGAQALERGIAILQYLEKSGGSSSVS
                     DISLNLDLPLSTTFRLLKVLQAADFVYQDSQLGWWHIGLGVFNVGAAYIHNRDVLSVA
                     GPFMRRLMLLSGETVNVAIRNGNEAVLIGQLECKSMVRMCAPLGSRLPLHASGAGKAL
                     LYPLAEEELMSIILQTGLQQFTPTTLVDMPTLLKDLEQARELGYTVDKEEHVVGLNCI
                     ASAIYDDVGSVVAAISISGPSSRLTEDRFVSQGELVRDTARDISTALGLKAHP"
     gene            complement(13409..13891)
                     /gene="allA"
                     /locus_tag="ACHKBL_002802"
     CDS             complement(13409..13891)
                     /gene="allA"
                     /locus_tag="ACHKBL_002802"
                     /EC_number="4.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HFO5819654.1"
                     /translation="MKLQVLPLSQEAFSAYGDVIETQQRDFFHINNGLVERYHDLALV
                     EILEQDRTLISINRAQSANLPLTIHELERHPLGTQAFIPMKGEVFVVVVALGDDKPDL
                     STLRAFITNGEQGVNYHRNVWHHPLFAWQRVTDFLTIDRGGSDNCDVESIPEQELCFA
                     "
     gene            14121..15047
                     /gene="allS"
                     /locus_tag="ACHKBL_002803"
     CDS             14121..15047
                     /gene="allS"
                     /locus_tag="ACHKBL_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AllS"
                     /protein_id="HFO5819655.1"
                     /translation="MFDPETLRTFIAVAETGSFSKAAERLCKTTATISYRIKLLEENT
                     GVALFFRTTRSVTLTAAGEHLLCQARDWLSWLESMPSELQQVNDGVERQVNIVINNLL
                     YNPQAVAQLLAWLNERYPFTQFHISRQIYMGVWDSLLYEGFSLAIGVTGTEALANTFS
                     LDPLGSVQWRFVMAADHPLANVEEPLTEAQLRRFPAVNIEDSARTLTKRVAWRLPGQK
                     EIIVPDMETKIAAHLAGVGIGFLPKSLCQSMIDNQQLVSRVIPTMRPPSPLSLAWRKF
                     GSGKAIEDIVTLFTQRRPEISGFLEIFGNPRS"
     gene            15116..16210
                     /gene="mnmH"
                     /locus_tag="ACHKBL_002804"
     CDS             15116..16210
                     /gene="mnmH"
                     /locus_tag="ACHKBL_002804"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043828 - tRNA 2-selenouridine synthase
                     activity [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HFO5819656.1"
                     /translation="MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMN
                     NDERAAVGTCYKQQGSDAALALGHKLVAGEIRQQRMDAWRAACLQNPQGILCCARGGQ
                     RSHIVQSWLHAAGIDYPLVKGGYKALRQTAIQATIELAQKPIVLIGGCTGSGKTLLVQ
                     QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENLLAAEMLKTDARQNLRLWVLEDES
                     RMIGSNHLPECLRERMTQAAIAVVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQ
                     EYCEYLHHGLSAIKRRLGLQRYNELAARLDAALTTQLTTGSTDGHLAWLVPLLEEYYD
                     PMYRYQLEKKAEKVVFRGEWAEVAEWVKTR"
     gene            16326..16691
                     /gene="ylbG"
                     /locus_tag="ACHKBL_002805"
                     /pseudo
     CDS             16326..16691
                     /gene="ylbG"
                     /locus_tag="ACHKBL_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014543172.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(17458..17556)
                     /locus_tag="ACHKBL_002806"
                     /pseudo
     CDS             complement(17458..17556)
                     /locus_tag="ACHKBL_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308588.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         4609 a         4576 c         4170 g         4201 t
ORIGIN      
        1 gatcacataa tccagatatt ttccctgtat gttaattgca gtcatgcttc acaccgtcgt
       61 taaaaaggaa gacagatgaa aatcagtcgg gaaacactcc accagctaat tgagaataaa
      121 ctctgccagg ctgggttaaa acgtgagcac gctgcaaccg tggctgaagt attggtttac
      181 gccgatgcca gagggatcca ctctcatggc gcggtgcgcg tggaatacta cgcggaacgc
      241 atttcaaaag gcggcaccaa ccgcgaaccg gagtttcgtc ttgaggaaac cgggccgtgc
      301 tcggcaattt tacatgccga caatgccgcc ggacaggtcg cggcgaaaat gggtatggaa
      361 catgccatca aaaccgccca gcaaaatggc gttgcggtgg tcggtatcag ccggatgggt
      421 cacagcggcg caatctctta ttttgtgcag caggcagctc gcgccgggtt aattggcatt
      481 tcgatgtgcc agtccgatcc aatggtggtg ccgtttggcg gcgcggaaat ttactacggt
      541 actaacccac tggcctttgc cgcgccggga gaaggcgacg agatccttac ctttgatatg
      601 gcgactaccg tacaggcatg gggaaaagtg ctcgacgccc gctcgcgtaa tatgtctatc
      661 ccggatacct gggcggtcga taaaaacgga gcaccaacaa ccgatccatt tgcagttaac
      721 gccctgctcc ctgccgccgg accgaaaggg tatggcctga tgatgatgat tgacgtcctc
      781 tcaggcgtct tactcggctt accgttcggg cgacaggtta gttcgatgta tgacgattta
      841 cacgccgggc gtaatttggg gcaattacat atcgttatta atccgaactt tttctcctcc
      901 agcgaattat tccgtcaaca tcttagccag accatgcgcg aattaaatgc cattaccccc
      961 gcgcccggtt ttaatcaggt ttattatccc ggacaggatc aggatattaa acaacgcaaa
     1021 gccgccgtcg aaggcatcga aattgttgat gatatttacc agtatttaat ttccgacgcg
     1081 ctttataaca cgtcatacga aacgaaaaat ccctttgcgc aataattatt gagacaggac
     1141 tttcttatga ttacacattt ccgtcaagcc atagaagaaa cgctgccctg gctttcctct
     1201 tttggcgctg acccagcggg tgggatgacc cgtttacttt attcgccgga atggctggaa
     1261 actcagcagc agtttaaaaa aagaatggca gcaagcgggc tggaaacacg tttcgatgaa
     1321 gtggggaatt tatacggtcg cctgagtggc accgaatatc cacaggaagt ggttctgagc
     1381 ggttcgcata tcgataccgt ggttaacggc ggtaacctcg acgggcaatt cggtgcgctg
     1441 gcggcgtggc tggcaattga ctggctgaaa acgcaatacg gcgcgccgtt acgtacggtc
     1501 gaagtggtgg cgatggcaga agaagaaggc agccgcttcc cgtatgtctt ctggggcagt
     1561 aaaaatatct ttgggctggc gaatcctgac gacgtgcgga atatctgtga tgccaaagga
     1621 aatagttttg tcgatgcgat gaaggcttgc ggatttactc tgccgaacgc cccactaact
     1681 ccgcgtcagg atattaaagc ctttgtcgaa ctgcatatcg aacagggctg tgtgctggaa
     1741 agtaatgggc agtcaattgg cgtggtgaat gcaattgtcg ggcagcgtcg ttatacggtg
     1801 acgctgaacg gcgaatcaaa ccatgcaggc accacgccga tgggttatcg tcgtgatacg
     1861 gtttacgctt tcagtcgcat ttgccatcag tcgatcgaaa aagcgaaaaa gatgggcgac
     1921 ccgctggtgc ttacctttgg caaggtggag ccgcgtccga atacggtgaa tgtggtaccg
     1981 ggtaaaacca cgttcaccat tgattgtcgt cataccgacg ctgccgtgct gcgcgatttc
     2041 acccagcagt tagaaaacga catgcgggcg atttgcgatg aaatggacat tggtattgat
     2101 atcgatttat ggatggacga agaacccgtg ccgatgaata aggagctggt cgccaccctg
     2161 acagaattgt gtgaaagtga aaaacagaac taccgggtaa tgcacagtgg tgccgggcac
     2221 gacgcgcaaa ttttcgcgcc tcgcgtgccg acctgcatga ttttcattcc cagcatcaac
     2281 gggatcagcc ataacccggc ggaacgcacc aatattaccg accttgccga aggggtcaaa
     2341 acgttggcac tcatgcttta tcaacttgcc tggcagaaat aaggagttat aaatgggata
     2401 tttaaataac gtcaccggtt accgcgatga tttactggct aaccgtgcga ttgtgaaaca
     2461 cggtaatttc gcactgttaa caccagacgg tctggtaaaa aatattattc cgggctttga
     2521 aaattgtgac gcgacaatcc tctccacgcc aaagctgggt gcctcttttg ttgattatct
     2581 ggtcacactg catcaaaacg gcggcaacca acagggcttc ggtggcgaag gcattgaaac
     2641 gttcctgtat gtgatctctg gaaatatcac tgccaaagcc gaagggaaaa catttgcctt
     2701 aagcgaaggt ggctatcttt attgcccgcc aggctcctta atgacgtttg ttaacgccca
     2761 ggccgaagac agccaaatct ttttatataa gcgtcgctat attccggtag aaggtcatgc
     2821 gccgtggctg gtttctggca atgccagcga actggaacac attcattatg aaggcatgga
     2881 cgatgttatt ctgctggatt ttctgcccaa agagttaggt tttgatatga acatgcatat
     2941 cctctctttt gcgccaggtg ccagccacgg ttatatcgaa actcacgtcc aggaacacgg
     3001 tgcctatatt ctttccggtc agggggttta taacctcgac aataactgga tcccggtgaa
     3061 aaaaggcgat tacatcttta tgggcgctta ctccttacag gccggttatg gcgtagggcg
     3121 tggtgaagcg tttagttata tttactcaaa agattgcaac cgcgacgtag agatttaagt
     3181 cataccactc gcctgattat ttttagctat gttggatttt gccacggttt attccgtggc
     3241 tttttttgta gtcaattcag gtaaaaaaag atgcgatata aatcgcatcc gctttaaagg
     3301 taagagtcag tttttaattc cctgacctat tttaatggcg caggcaatat ttcgcgcgct
     3361 gttaaagaga ttggtttcac cgctggcgag cacttcggct agaggtgcca gacgtggcaa
     3421 aatgctgaat accgcgtcaa tgccgtactg gtgcaccact tccacgccat cacccaatac
     3481 gccagcaatc ccaatcaccg ggacattaaa ctgcttcgcc accgacgcca cgcccagcgg
     3541 cgctttacca cctgccgttt gcgagtcgat gcgcccttcc ccggtgatca ccagtgctgc
     3601 gccctgcact gcctgcgcaa gattgaccgc attcaacaca atctcaatac ccggtttaat
     3661 atccgcattg aggaataccg ccgccgcaat gcccataccg cccgccgcgc caccacccgc
     3721 catctggcag acgttgattt cagtttgctg ttgtaaaaca cgggcgtaat tttgcagccc
     3781 ctgctccagc tcttcgacca tttccggcgt tgcccctttt tgcgggccga acaccgccgc
     3841 cgcgccgcgt gcccctacca gcggattatc aacatcacat gcgacttcaa tatggcagtt
     3901 cgccagacgc ggatcgcatt catccatctc aatacttgcc acgcgtgcta aattgccgcc
     3961 gttagccgcc agcacctgac cgtaggcatc aaggaaacgc acaccgagcg cctgcgccat
     4021 gcccataccg ccgtcaaccg tcgcgctacc accaatgccg agaataatat ggcgaatgcc
     4081 gttatccagc gcatggcgaa ttaactcccc cgtaccaaaa ctggaggcca gcaacggatt
     4141 acgcttttca ggcgcgacca gcatcaggcc acttgctgct gccatctcaa tcaccgccgt
     4201 tttcccgtcg ccggtaatgc cataaaaagc attcactttt tcgcccatcg gcccgcagac
     4261 ttcaagcttc acgatgttac cgtccgtcgc ggcgaccatc gcctccaccg tcccttcgcc
     4321 gccatccgct atcggcaaac agatatagtg cgcatcggga aagagggtcg aaaacccggc
     4381 tttaattgcc tgacaacatt tttctgcact taagctctct ttaaaagagt ctggcgcaat
     4441 gacaatcttc attatcaatc ctttttcaac aaattattcg atgcgttccc acggaataag
     4501 attatccata agaacagcca gtaaaattcc gaccagtaag ccgttactga gcagaggacg
     4561 aagcgttaat ggaaggtctt gcagatacac aggcggtaat gccattaaaa atatgccgac
     4621 aaataacggc aatgcgagtc gataaatatt gcgagcggta aacgtgattt gctggctaaa
     4681 caccagcgcg gaaaagagta aaggcagata agaaaccagc atgaccgcgc tactcactgg
     4741 taaagggata ctgcaaaaca gtcgcgtgag tgccggaacc agtgccacca gcaggcaaat
     4801 aacgctgcca taaataaacg aacgccgcgt gtaatcgcta gtctgggtta ataaaccaat
     4861 ggatgaaaca aacggtgaaa atggaattac tgccagtggc acggttatca gtgtcataaa
     4921 tccggtcgcc acaaagctgc gacgataacg cgtattcccc gcgccctgct gcggataaaa
     4981 aacatccgtg ccccgaatcg caccgtaggt attgctgata tttaccagac ctgtaatcac
     5041 cgccgtcaga ataatccccg gcgacaaagc accaccactg ccgagcggga accactgcca
     5101 gtgcaactca ccggagagcg aatgcgaaga aggaaagcaa aagtaccaca acaaccagcc
     5161 ggttatcgta ccgaccagca ggccgtaacg ggcaaaacgt tgtggcagga aaataatcat
     5221 cgccagtacc aggcacatca ccgcgaccga gagcgcgaac ggcggtaatt gaattttaaa
     5281 attcgggtcg gctatgccaa acggcagccc gagcatacct ttgaaaaaga tagtggtcag
     5341 ttgcgcaccc agcatcaaca taaacaagac catcaccgac ggcgtaaata accgtgccag
     5401 gcgatgtcct aatccgctaa aaccaatcaa catcgtcagc acgccggaga gcgcaattcc
     5461 caccgccaga ctggtagcga tatcgttgat cggtgtcccg cgcgatgctt cgccaagggt
     5521 gatagtcagg atggttcccc accacaggcc gcccggcccc tccataatag cgcgacgatg
     5581 accgcagaac gcctgagcga agcaggccag tgcggtagca agaaaagcat attgcgtgag
     5641 cgtaagcagg ctactttgcg gcaactgaaa agcagaaagt agcgtaggag gaaccacaac
     5701 cgtgttgcaa aaaataaaga aaaaccactg aaatcctgat aacaggcttt cgcggctgac
     5761 tgcaaaattg aacataaaaa caccttaacc ggagaatgcc cgccgcaagg acgggcattg
     5821 caggggccag attactgctg atgtttaagg ataaattgac ctttcggcgc aacagggaag
     5881 ccctgttcga tgtcgtaaat cacatcacca cgtaagatgg ttttcgtgat acgcgcgcca
     5941 atggtacggc caacatacgg gctgactttg tggcgatatt ccagatcgtc attggtaaga
     6001 acatagctgc tattcggctg aatgaagacg aagtcggcat cttttcctgg ggcgatacgg
     6061 cctttttgct gcagaccgaa aatatctgct gcgttagtcg ccattaattt gccgaacatc
     6121 ggcagagaca taccgcgttt ctgtaccgct tcatcgaaca tcacgtccat gcagctttgc
     6181 agaccggcga taccacccca tgctttcatg atattgccgg ctttcatttc cggcggacat
     6241 ggagagtggt cggaaaccag gcagtcgatt tcaccgttaa acagtttttc ccacatgcct
     6301 ttctggtttt ccagatcgcg gatcggcggt gaacacttcg ccagagtacc gatttcttcg
     6361 aactgatcgg tatcaagtac aaagtaatgc gggcaggatt cacaagtaac gtcctgacct
     6421 tcctgacgtg cacgagtcac ttcttcaacg ccttccgggc tgctgatatg gcaaacgtgc
     6481 agacggcaac cggcaacttt cgccagatac agcacgcggc gaatcgcttc cacttcggta
     6541 aataccggac gcgaagccac atagtcatga gcagttacgc gaccttcacg cttcgcttct
     6601 tcgcccagtt cgtcacagat cagcgcgttt tcgcagtgca ccagcaccgg ctgccccagt
     6661 tcgcccagct tctgcgcacc tttgaagaac tgccagtcgt ttacatcacg gaagtcgttg
     6721 tcgataccgc gatcgccaca ggtcgcaacg aagcatttga agccgacaac gcccacttca
     6781 tccagctcat gcagacggtc gatgttgtaa gacaccaggc caccgagttg tgccgcatcg
     6841 atagtcagct tgcctttagc ggcatcgaac ttcagttcaa ttgaagcgcg gtcaaccgtt
     6901 gctggcagct ggttgagcgg catttcgatc atggtggtga taccaccttt tgccgctgcg
     6961 cgagtaccag tttcataacc ttcccagtgg ctacgacccg gctcagaaat atgggtgtgc
     7021 gcatcaacca tgcctggaga aaccaccaga ccagacgcat ccatcacttc ttttgcatcg
     7081 cccagatcct gaccgatagc agcaattttt ccgcctttaa cggcgatatc tacaacgcga
     7141 gcttcgtttt ctaaaataac ggtgccgttt ttaatgatta aatcaaaaga cataacaaac
     7201 tccttgtaaa atacagattg gttttttaac tcaatgttca atatcgggat taattaacct
     7261 gtgacttttt gttctcctgt tttttctgct gctgtacgtt tttttaataa cgcgtaggca
     7321 gcgaatgcga caataacgcc gacaaaccat gaaacgcgtg ataaaggctc catgaacgga
     7381 ataaatttac caccgagaga taaaataacg gcgaccaaag ttactgagaa cgcggtaagg
     7441 ttaaagccgt tatcgtaata tttgaaatca cctgctgcgg tatacagttc atcaagatta
     7501 atttgtccgc gcatcaccat aaaataatgc gccatcatga caccaattac cggacccagc
     7561 attccgccga taatatcgag gaacagataa atactgtcct gattttccat taatttccac
     7621 gggcagatca ccaggctgat aatactggca atcagtacgc cgtttttata ggtcagtttc
     7681 gtcggtgcaa tagcggcaat ctgataaccg gccggaataa tattaccggt ggcgttagtg
     7741 gaaatggtcg tcatcagaat aaccagtacc gcaaagaacg aggcgaacag gctgtcccaa
     7801 cgctgaacaa tatccagcac gttccaggtg tcagcgccgt aatgaatact ggctccggca
     7861 ataatacata ccccggcgac cgcaaacaga atataggcca caactaaacc cagcgtttgc
     7921 cccagcgcct gctcacgaaa cgagtgggcg ttttgcgtaa agtcggatgc gctcaccgcc
     7981 ggtgccgccc agaccgcaac caccgcgttt atcaccacca gaaacaggaa gccactgttt
     8041 tctgctttct gaataccgcc cggaatgtag tcaaagattg gaccgatccc gaccagcgaa
     8101 atcgcccaaa tcgccatacc gccgaaaacg atatagatgc acgggttaag aatggcagtg
     8161 aatttattta aaacttttcc gccgccaaaa ccaataccaa cgttaaccag ccagaagagt
     8221 aaaaaagtaa ttaatcccgg tagagaaagg cctaacagcg tgaaatcgcc accgagagtc
     8281 aaaaatcccg gccagatttt gccaatcaga atcaagcagg ccagtgaccc cgcgtaacat
     8341 tgcaggccaa accacataat ggcagcaata ccgcctctta ataatccggg aaacagtgca
     8401 ccacgtacgc cgtaagaagc acgcaggatc atggcaaacg gtacgccgta tttactgccc
     8461 gcagcaccgt ttaataccat taccgcggca atgaaaaagg cactgaggat aattgccagc
     8521 ataatactaa aggtagacaa gccgagaata aaaaagccgc caaccatcac ataattggga
     8581 acgttatgaa ccgaacccat ccataaggta aaatagttaa atgttttcca ggtccgctga
     8641 ctttgcgttt tcggcagtag atcttcgcta tagccgcgtt gctggaatag ttttctctga
     8701 tgttccataa atccctctta atttcacgta tcgaataacg ttgttttaat ctagctatga
     8761 ggaacaaata aaataaaaat attattttta atactgaagc aacatctttg gctgctttct
     8821 ttttttaata ttgctggccg attcattttc tgatatcggc catttttatt gcgggtatca
     8881 ggccagttta tggttagcca ttaattccag cgcctgcact aatgcagagt gatccaactg
     8941 gctgccaccg tttgccgcac aggtattaaa caattcctgg caggtcgcgg tgtttggcag
     9001 gttcagcgcc agtgctttcg cactttgcag tgccaggttg agatctttct ggtgcagcgc
     9061 aattttgaaa cctggattaa aggtgcgttt aatcatgcgc tcaccgtgaa cttccagaat
     9121 acgtgaggaa gcaaagccgc ccatcagcgc ctggcgcaca cgtaccgggt ccgcaccggc
     9181 ttttgaagca aacaacaagg cttcagaaac cgcttcaata ttgagcgcca cgataatctg
     9241 atttgccact ttgcaggttt gaccgtcgcc gttaccgccc acgagagtga tatttttacc
     9301 gagcagttca aacagcggtt ttacacgttc aaatactgct tcatcaccgc caaccataat
     9361 cgataacgtc ccttcacgcg cgccgatttc accgccggag actggcgcat cgagataatc
     9421 gccgcccagt tcattcacct gacgagcgaa acgtttagtt tcaatcgggg aaatggagct
     9481 catatcaaca atggttttgc ccttcagcga ggctttggta caaccatttt caccaaacag
     9541 aacgtcttca acctgtggcg tatccggcac cataataaaa atgatgtccg atgcatctgt
     9601 tacctggcgc gcagtttcaa cactaacggc gcccagtgac agtaattcat cagcaaccgg
     9661 accaatggtt gtgacatgca gttgatgacc ggcacgcgcc agattaatgg ccatcggtgt
     9721 acccataatg cctaagccaa taaatcccag tttcataatt aacctctttt aaatttcgct
     9781 tttcctgaat tcagacaaca cgatcccctg ccaacttagc ggcctaaaaa agcattgtct
     9841 gaatagcgtt acgttttaac ggtacggatc catccagcgt aaaccggctt ccgtggtggt
     9901 ttggggttta tattcacagc caacccaacc gttgtagtca gaattttcaa ttaccgtaaa
     9961 gagatattcg taattaattt ctccggttcc cggttcgccg cgatgcggat tatcagcaat
    10021 ttgcaggtga ccaattttat cggcccactg agtcatggta ttagttaatt caccttccat
    10081 ccgctgcatg tgataaatgt catactgaat tttcaaattg cagcaaccaa catcgtcaat
    10141 cagtttcagt gcctgccggg ttccggtgaa atggaaacca ggaatatcaa aatggttaat
    10201 aggttcaatc agtaataaaa tatcttcttt catcagcata ttcgcagcat aacgcaggtt
    10261 ttctacaagc gttgtgtgaa tctgttcact gctgaaacca gtcggcgttt taccgaccag
    10321 acagttaatt tttttattac ccagagcacg agcataacga atcgctgctg ctacgccatc
    10381 ccgaaactct tcttcacggc caggaataca ggcaataccc cgctcgcccg ccgcccagtc
    10441 acctgccggt aaattgtgca gcgtatgttc gagtttatta ctcgccagca cctgttttaa
    10501 ttcttcaatg tcgtagtcat aagggaacat aaattcaacg ccgcgaaaac cacactgcgc
    10561 agctttctca aaacgggcga gaaaatcata ttcaccaaat aacatcgata aattagcaga
    10621 gaaacgtaac attatttatc tcccttattc atagtgcatg aaacaggctt cagtcggtgc
    10681 atccgctgcg ttatcggcga tatcttcaaa ttccatgacg ttatccagtt cactgcccat
    10741 cgaaatattg gtcacacgct cgagaataac ttccacgact accggtaccc gatattgcgc
    10801 cattaagatt ttcgcctgtt caaaggctgg tgcaatatct tccggtttga aaacgcgaat
    10861 tgctttacaa cctaaacctt ccgctacttt tacgtggtcg acgccataac cgttcacttc
    10921 gctggagtta atattttcga acgcgagctg tacgcagtaa tccatatcaa aggcacgttg
    10981 cgactggcga atcagaccca gataggcgtt attgaccagt acatggatgt acggaatgtt
    11041 gaactgtgcg ccaacggcta attcttcaat caggaactgg aagtcgaagt caccggaaat
    11101 cgccaccaca ttgcgctctg gatcagcggc acaaaccccc agcgccgccg gaatcgtcca
    11161 gcccagcggg cctgcctgcc cacagttgat ccagtggcgg tctttaaaga catgcagcat
    11221 ttgtgccgca gcgatttgtg acaggccaat ggtggtgaca taacaaacat cgcggccaaa
    11281 ggctttgttc atctcttcat acacgcgctg cggtttcacc ggcacgttgt cgaagtgggt
    11341 tttgcgcagc aaagtgcgtt tgcgctgctg gcagtcggcg acccattctt tacgacacgg
    11401 cagacgaccc gctttttgca tctcctgcgc cacttcaacc agcagcgtca gcgccgcttt
    11461 agcatcagag acaatgccga gatccggaca cagcacgcgg ccaatttgcg tcggctcaat
    11521 atcgatatga acgattttgc gcccttcggt gtatttctct accgaaccgg tatgacggtt
    11581 agcaaaacgg ttaccgatac caaacaccat gtcggacgcc agcagcgttg cgttaccgta
    11641 acgatgcgcg gtttgcagac ccaccatccc ggccatcagt tcatgatcgt cgggaataca
    11701 gccccagccc atcagcgttg ggatcaccgg aatgctggtc agttcagcaa actgttgtaa
    11761 cagtgcagct gcgtcagcat tgattacccc gcccccggca acaatcaccg gacgttcggc
    11821 ctggattaac atttctaccg ctttttcgat ctgcatacgg ctggcagcag gtttgtagac
    11881 cggcagcggt tcgtacatgt caggatcaaa ctcgatttcc gcaacctgaa cgtcgaacgg
    11941 taaatccacc agtaccggac ccggacgacc agaacgcatc aggtgaaatg cctgttgcag
    12001 cacgcgaggc accagcgccg cttcacgtac tgtaaccgac attttgctga ccggtttagc
    12061 aattgcttca atatcaacgg cctggaaatc ttctttatgc agacgggcgc gcggagcctg
    12121 gccggtaata cacagaatag gaatggaatc agcagaagca gaatagagcg cggtgatcat
    12181 gtcagtgcct gctggtcccg aggttcccag acatacgccg atattccctg ccgttgcgcg
    12241 ggtataacct tccgccatgt gcgaagcacc ttccacatga cgcgccagaa tgtgacgaat
    12301 accgccgtgc ttacgcatcg ctgaatagaa aggattgatt gctgctcccg gaacgccgaa
    12361 ggcggtggtg ataccttctt tctccagcac atacattgcc gcgtcaacgg ctctcatttt
    12421 tgccatttta ttcctacctc tattaattgg aaaaattttc caactttcaa attttgtcaa
    12481 aaaaacccgc tccgcagagc gggatgcgac agacactatg gatgtgcttt cagtcccaac
    12541 gccgtgctga tatcgcgggc ggtgtctctg accagctcac cctgactgac aaaacgatct
    12601 tctgtcagtc ttgatgaagg cccggagatg gagatagcgg caacaacact acctacatca
    12661 tcgtaaattg ctgaagctat gcaattcagg ccgacaacat gctcttcttt atctacggta
    12721 tagcccagtt cacgcgcttg ttccaggtcc ttcagcaagg tgggcatatc cacaagcgta
    12781 gttggcgtaa actgctgcaa accggtttgc agaatgatgc tcatcaactc ctcttccgcc
    12841 agcggataaa gcagcgcctt gcccgcaccg gaagcatgca gcggcagacg actgcccagc
    12901 ggcgcacaca tcctgaccat cgatttacac tctaactgac caattaatac cgcttcattg
    12961 ccgttacgga tcgcgacatt gaccgtttcg ccggaaagta acatcaggcg gcgcataaac
    13021 ggcccggcga cggagagtac atcgcggtta tggatgtacg ctgcaccgac gttaaagaca
    13081 cctaatccta tatgccacca gcctaactga ctgtcctgat agacaaaatc cgctgcctgt
    13141 aaaaccttca gcaagcgaaa ggtcgtggag agcggcaaat ccagattgag agaaatatcg
    13201 ctaaccgacg aacttccccc acttttttcc agatattgca gaatcgcaat tccccgctct
    13261 aacgcctgtg cgcccttctg tgccacaggc tccgcctgtc ctggcctgcc gcgccgtcta
    13321 acttccgtca tacctacgtt aactccgacc tacaagtgag cataaacaat gaacaattcg
    13381 tttatcttat gcaagtcggt tgcaggcgtc acgcaaaaca gagttcctgt tcaggaatac
    13441 tttcaacatc acagttgtca ctgccgccgc gatcgatggt cagaaaatcg gtgacgcgct
    13501 gccaggcgaa aagtgggtga tgccagacgt tacgatggta attcaccccc tgttcaccgt
    13561 tggtaataaa cgcccgcagc gttgacaggt ctggtttgtc gtcacctaac gccacgacta
    13621 ccacaaacac ttcacctttc atcgggataa acgcctgtgt acccaacgga tgacgttcga
    13681 gttcgtgaat agtcagcggc agattcgccg attgcgcgcg gttaatgctg ataagcgtac
    13741 ggtcttgctc aagaatctca accagcgcca aatcgtggta acgctctacc aggccattgt
    13801 taatatggaa aaaatcccgt tgctgcgttt cgattacgtc gccataagca ctaaaggctt
    13861 cctgacttaa cggtaatacc tgaagtttca tctttttgtc cttgtaacat aattccgttc
    13921 aaccaccgcc gaccggtcag cttggtttca ataacaggca atctaaaacc ttaagagaac
    13981 aacatcaata caaaagttgg aatatttttc caaaactaga tttagtccac aaattgaaca
    14041 gggttatcat gtaaaaatac tgataaatca gaggataaga aaatcacgca acttgcggat
    14101 ttaaaagggg atacctgaag atgttcgatc cagaaacctt gcggactttc attgcggttg
    14161 ctgaaacagg aagtttttca aaagcggcag aacgattatg taaaaccacg gcgacgatca
    14221 gttatcgcat taaacttctg gaagagaata ccggagtagc gctgtttttc cgtacgactc
    14281 gtagcgtgac gttgacggcg gctggcgagc atctactttg tcaggccaga gactggctga
    14341 gctggctgga aagtatgccc agcgagctgc aacaggtgaa tgatggcgtg gaacgccagg
    14401 tgaatattgt catcaacaac ctgctctata acccccaggc cgtcgcccag ttgctggcgt
    14461 ggctgaatga acgttacccc tttacccagt ttcacatctc ccgacaaatc tatatgggcg
    14521 tctgggattc gctattatac gaaggttttt cactggctat cggcgtcacg ggaaccgagg
    14581 cgctggcaaa tacctttagt cttgatccct taggatcggt gcaatggcgc tttgtcatgg
    14641 cggcggatca tccgctggcg aacgttgaag agccgctaac agaagcacag ttgcggcgct
    14701 ttccggcggt taatattgaa gacagcgccc gcacactaac caaacgcgtc gcctggcgat
    14761 tgccggggca aaaagagatt attgttcccg atatggaaac gaaaatcgcc gcccatctgg
    14821 cgggcgttgg cattggtttt ttgccaaaat cactttgcca gtcaatgatc gataatcaac
    14881 aactggtcag ccgggtaatc ccaacgatgc gccctccttc accattgagt ctggcatggc
    14941 gcaaatttgg cagcggcaaa gcgatagaag atattgtgac cttgtttacc cagcgcaggc
    15001 cggaaatcag cggattttta gaaattttcg gcaacccacg cagttaaaaa tcaagtttcc
    15061 ttgcgcgttg gcgcgtaaaa tagcgcgcaa gctgatttga ggttcacttt ttactatgca
    15121 agagagacac acggaacagg actatcgtgc cctgctgatt gctgatacgc ccattattga
    15181 tgttcgcgcc cccatcgagt ttgagcaagg cgcaatgcct gccgctatca acctgccgtt
    15241 aatgaataac gatgaacgcg ccgccgttgg cacctgctat aaacagcaag ggtcagacgc
    15301 ggcgctggcg ctgggtcata aactggtggc gggtgaaatt cgtcagcagc gtatggacgc
    15361 ctggcgggca gcgtgcctgc aaaatccgca aggtattctc tgctgcgccc gtggcggtca
    15421 gcgctcacat attgtgcaaa gctggttgca tgcagcgggg attgattatc cgctggtgaa
    15481 aggcggttat aaggcactgc gccagaccgc gattcaggcg actattgaac tggcacaaaa
    15541 accgatagtg ctgattggcg gttgtaccgg cagcggtaaa acgctgttag tgcagcaaca
    15601 gccgaacggt gttgatctgg aagggttggc gcgtcatcgc ggttcggcgt ttggtcgcac
    15661 cttacaacca caacttagcc aggcgagttt tgaaaacctg ctggctgccg aaatgctaaa
    15721 aaccgacgcc cgtcagaatt tgcgcctgtg ggtgctggaa gatgaaagcc ggatgatcgg
    15781 ttcgaatcac ctgccggaat gcctgcgcga gcgaatgacc caggcggcga ttgcggtagt
    15841 agaagatccg tttgagatcc gtcttgagcg gctgaacgaa gaatatttcc tgcgtatgca
    15901 tcatgatttt acccacgcgt acggcgacga acagggctgg caggagtatt gcgaatacct
    15961 gcatcacgga ctttcggcga ttaagcgtcg gctggggcta cagcgctata acgaactggc
    16021 tgcaaggctg gatgcggcgc tgacaacgca acttaccacc ggcagcaccg acggtcatct
    16081 ggcctggctg gtgccgttac ttgaagaata ttacgacccg atgtatcgct atcagcttga
    16141 gaaaaaagcg gaaaaagttg tctttcgcgg tgagtgggcg gaagtggcgg aatgggttaa
    16201 gacgcgataa tgagggacgt atttgtatta gtcgcgactt gatctgacac atggccttga
    16261 aaggttgaga gttaccggtt ttgatatgag tgtcgaatcc ttatccaaaa caagaggtaa
    16321 ctctcatgct tcatactgcc aatcccgtca tcaaacacaa agccggtttg ctcaatctgg
    16381 ctgaagaact cagcaacgta tcaaaagcct gtaaaatcat gggcgtttcg cgtgatacgt
    16441 tttatcgtta ccgcgaactg gccgctgaag gcggtgtgga tgcgcagatt aatcgtagtc
    16501 gccgcgctcc taaccttaag aaccgtaccg atgaggcaat tgaacaggct gttgttgatt
    16561 acgccgtcgc tttcccgaca catggtcagc accgggccag caacgagctg cgtaaacagg
    16621 gggtttttat ctccgacagt ggtgtccgtt ctgtctggtt actccataac cttgagaatc
    16681 tcaaaagacg atactgaaca aattttatca ggtgacgttc cgtaaaaagt tgtatggagt
    16741 tctcgatgca gtcggcgcga ctatcagtac ccccgctgaa ccctggacta tcgggccgcc
    16801 gtactggggc atgtcaccct ggcgtgccgc tggttttccg ctaatggttt atttcctctt
    16861 tctttcccgg cgagaattta ttttaatccg gaaggattat ttattttcag atagggactc
    16921 acattaaaca cgtaacattt taattaatgc atggctgaat atgtattaca caatgtcaga
    16981 tatcgtaaaa gaccagtgca attctatgta aactctctgg ctgttcattt tatttgttgt
    17041 ttcagggtcg gaactcaccc tcaataaaca actgaatacg agtagatatt catcgtctga
    17101 gctatatggc tttacacaat agccgacatg tcttttctca gtcagggatt atacttttcc
    17161 tcactacgaa gttgacgact tcgatatggg atagactctt aattcaagca attactctgc
    17221 acaagcatca ggtaacccag ctccaatatt tgttataaaa taatgcgtaa cattaatttc
    17281 accaacaaaa aaaaggttta cgctatcccc ttgccctggt attttaccat atttacacca
    17341 cacttttttt gtatctgtat cattctctga tacgattttc gccacttgtt ccctggccct
    17401 cacagtcaat gttttcttaa acctcccccc atcatcgatt gttagggaaa aataagaaaa
    17461 aaaataatca tcttctacat ttgtggatat tgatatatcc accgctacgt gcggaaaaaa
    17521 atctgggagc catgtaaaaa ccccatcact ttctgg
//
LOCUS       DAZFLD010000101        17502 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.102, whole genome shotgun sequence.
ACCESSION   DAZFLD010000101 DAZFLD010000000
VERSION     DAZFLD010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17502
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.102"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(149..451)
                     /locus_tag="ACHKBL_002807"
     CDS             complement(149..451)
                     /locus_tag="ACHKBL_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001535197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5819657.1"
                     /translation="MSHCTSDGRFVCNDGSLSQSKRFCSGYGVAPAKRQVKSPVTTKL
                     VQKKKSAAVRKVSQPSVAGKNEVVPVTEPRKPTCAPLYMASKPGFTHLPICSGNQY"
     gene            complement(798..1802)
                     /locus_tag="ACHKBL_002808"
     CDS             complement(798..1802)
                     /locus_tag="ACHKBL_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001179707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFO5819658.1"
                     /translation="MQNITLIGIDLGKHSFHIHCQDKFGKALLRKKFTRTKLMQFLAT
                     CPPSVVVMEACAGAHFMARRISDFGHETKLISPQFVRPFVKSNKNDFVDAEAICEAAS
                     RPSMRFVQPRTEPLQAMRALHRVRESLIRDKVKTTNQIHGFLLEFGISLPTGDAVIKR
                     LSLVLAEHEIPEYLSRLLVRLHTHYLYLVEQIAELESELSQSINADDTAQRIMTIPGV
                     GPITASLLSSQPGDGKQFSCSRDFAASTGLVPRQYSTGGKSTLLGISKRGDKNLRRLL
                     VQCARSFMMRLERQHGKLAEWVREQLNKKHSNVVACALANKLARIAWVITTQQKEYQA
                     "
     gene            complement(2060..2491)
                     /gene="tssE"
                     /locus_tag="ACHKBL_002809"
     CDS             complement(2060..2491)
                     /gene="tssE"
                     /locus_tag="ACHKBL_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HFO5819659.1"
                     /translation="MPRPSLYEILYGNFAGGLELNQVSAEEQVILSVLDNMQRILNTR
                     AGSLKHLPDYGLPDMTTVLQGMPGTAHQLMRVLSDVLLKYEPRIKRVNVTMQEQTQPG
                     ELHYVIDAELKDAGLVRYGTTFIPEGRVLLRHLKQQRYVQT"
     gene            complement(2494..3030)
                     /gene="tssJ"
                     /locus_tag="ACHKBL_002810"
     CDS             complement(2494..3030)
                     /gene="tssJ"
                     /locus_tag="ACHKBL_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000974467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system lipoprotein TssJ"
                     /protein_id="HFO5819660.1"
                     /translation="MAIIAGKAGYGLIIALFSLSLSGCGLTQRVADGTVSATKSLFYR
                     QIKTLHLDIRAREAINTSAAGIPLSVVVRIYQLKDNRNFDSADYQALFTGDSEILAGD
                     IIAQKDVWMQPGGSVTVDMPLDDAAKFTGVAAMFLEPDRKKNTWRVVLSRDELEPDTP
                     RLIEVSGNTLTLLPVKDK"
     gene            complement(3011..4111)
                     /gene="tssG"
                     /locus_tag="ACHKBL_002811"
     CDS             complement(3011..4111)
                     /gene="tssG"
                     /locus_tag="ACHKBL_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HFO5819661.1"
                     /translation="MHPVERKSQSAPARLITRYRKQLPYINFYRFCQLLEQSQPDQPP
                     IGSGWQARQEAVRFCPYTGMGFPASEIKDAVIPEESHLPPTVHVTFMGLYGVTSPLPA
                     HYISDIAQQREGHEAAADFLDIFSHRLITQYYRIWRKYSYPATFEAGGQDKTSQYLLG
                     LARLGIPGCAQNIATPVSRFLALLPLMLLPGRTAEGLTSLVTLLAPGTQARVWHHDRR
                     RIPLKTPLAMRVHHPVSLKSRPVMGDHATDVNGQVLLQLSTQTGSEVQGWLPGGQLYS
                     DLLALLHVYLGSRLDVRLQLCVERSLLPDVRLSCRPAAGSPQLGRTAVMRTQAKITTS
                     AARVMTIRLGRYQRVQEHYQRKEAQENGDYRW"
     gene            complement(4066..5829)
                     /gene="tssF"
                     /locus_tag="ACHKBL_002812"
     CDS             complement(4066..5829)
                     /gene="tssF"
                     /locus_tag="ACHKBL_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HFO5819662.1"
                     /translation="MDDLTLRYFDAEMRYLREAGKAFAQAHPDRAAMLDLDKAGTPDP
                     CVERLFEGFAFSMGRLRQKIDDDLPELTESLVSMLWPHYLRTIPSLSVVALTPRLSVM
                     KMAETVPAGLEVTSRPVGPGNTVCRYRTTRAIPQNPLAVEKVVMTTEPDGRSVLKIGF
                     ACSELADWSQVDLHRLSLYLAAEAPVSSTLHLMMTKRLAALYLRLPGNDERIRIDGWF
                     SPGGFAEEDRLWPKGDSAFSGYQLLLEYFTFREKFMFVHLNGLENISLPAGISGFDIE
                     VVLSQSWPADLPVTDDALCLHCVPVINLFTLEADPLIINGLESEYLLRPKRLQDGYTE
                     IYSVDAVAGSGRTGSAEYVPFTSFRHRGGMLRHDAPERYYHTRVKRGVTGMYDTWLIL
                     GGQRWEADRMPERETLSLRITGTNGQLPRRALQSTLLDRCEQVLQTPISVCNLCKPTL
                     PVYPPTEDRFHWRVMSHLGTGFLNMLSSAEVLRGTLALYNWRDDELNHRRLDAILAVQ
                     HHRIQRFEKGFLLRGLDVEVTLDGNGFAGEGDIHLFGEMLNRFLALYADMNQFNQLTL
                     IVQPEGKCIRWKENHNPRLPG"
     gene            complement(5963..7561)
                     /gene="tssA"
                     /locus_tag="ACHKBL_002813"
     CDS             complement(5963..7561)
                     /gene="tssA"
                     /locus_tag="ACHKBL_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssA"
                     /protein_id="HFO5819663.1"
                     /translation="MASIHSLLSACQTTPRDVAEPAQVRIALWDKWLAPVTPDNPAGD
                     DAGYDDDFQQMREEVNKLSGADAGIVSQLAEKLLTTRTKNIRVATWYIWARLRQDGEK
                     GLADGLELLTGLLQRFGEHLHPQRSRARKAALEWLCSARILDSLSLYPEVVKADTLRI
                     AGALWLAEQTFTDEASAPVLNGLYQALENRLMKAGGVDAVVPQEAAAPAPTVTSGSVM
                     ALSAITSGQELLSQARVLAKYLRDQPEGWLAAHRLMKSVRHDTLHQLPPLSADGRTRI
                     APPGPDRRASLKRLYLQQNWLSLLEQCDDMFARGASHLWLDLQWYIHQALLQTGKENY
                     AAIIQYDLKGLLLRLPGLETLAFNDGMPFADDVTLSWIQQQVMECGERWAEEPSVTIT
                     AAPGDNDILSLEPEALQIADNEGTEAALSWLQARPGIQSDRSNWLLRLLMARVAEQTG
                     KNDLALHLLVELDERATRLTLSQWEPELVFEVKARRLKLLRMKSAKTESDRVRLQPDM
                     EHLLAGLIAIDAARAAVLCNSGSS"
     gene            complement(7561..10950)
                     /locus_tag="ACHKBL_002814"
     CDS             complement(7561..10950)
                     /locus_tag="ACHKBL_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImcF-related family protein"
                     /protein_id="HFO5819664.1"
                     /translation="MNKLACLSGRFGRPGIVFIGVAALWWLITRYGAFLGAETRRDQI
                     LLLILLSLGLLFVCYLPVMKKYVQELTYRRRARKEQRLPDDEERLAQTSPRYVTVQDI
                     RHTLRRQHGRFWGRKIRILLITGTASEVELLTPGLTEQFWQEEQGTLLLWGGDPSQPE
                     NADWLAALRRLRYRPADGIVWVTSGLSETLSAPLTEDALDRVSRAVSSCCERLGWRLP
                     LYVWSLQESPDERGRITQPVGCLLPAECSSDKLKAQLQAMLPGLVAQGIQQICCAPRY
                     YFLLSLAERFRRNIDAVVEPLSVLLRPYRQLLLAGIVFSPATVGGERSVRHRWRMDNR
                     WEALPETVQQLPVRLQPSRTGHNWRRSLAVMAAILMMAQGTGMVVSFLANRSLVAEVQ
                     EQIRPAQNQQLSPAERLQALLNLQKSLARLQYREEHGAPWYLRAGMNQNADLLAVVMP
                     LYAQNAHLLLRDAAAAHLEQQLRTFIRLPPDSPQRGKMAKAAYDQLRLYLMLAQPQHM
                     EPARFSRTLMREWPQRDGVSAVFWQANGPTLLAYYASGIITHPQWKLTADEELVSQSR
                     TLLLRHLGTQNSDAMLYQKMLARVAHQFADMRLTDMTGDTDVSRLFFTDEVVPGMFTR
                     QAWEEAVLPSIDTVINERREEMDWVLTDGRQKAPSPVSPEALRQRLTTRYFADFGNAW
                     LNFLNSLHLRKAQTLSDVTEQLTLMADVRQSPLVALMNTLAVQGRTGQPREAVTDSLV
                     KSARNLLSQEKQPVAVPESRLHGPLATTFGPVLALMDNQNNSADMLNLQTYLTRVTQV
                     RLRLQQIAGSSDPQAMMQMLAQTVLQGKSVDLTDTRDYGSLTAAGLGQEWYGFGQTVF
                     VRPMEQAWQQVLTPAAESLNARWRTAVVDGWNNAFSGRYPFKNVSSDASLPLLAKYLN
                     TDTGRIARFLQNNLSGVLHSEGSRWVPDTINTRGLTFNPAFLKAINTLSEIADVAFTT
                     GNAGLHFELRPGTAAGVMQTTLITDNQKLIYVNQMPVWKRFTWPADTEAPGASLSWVS
                     TQAGTRQYADLPGSWGLIRLLEMARRKAAPGVASGWSLSWQAQDGRMLNYTLRTEAGE
                     GPLVLLKLRNFVLPETVFELSGTSAFTGNDEDAGDSVEETD"
     gene            complement(10943..12091)
                     /locus_tag="ACHKBL_002815"
     CDS             complement(10943..12091)
                     /locus_tag="ACHKBL_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein"
                     /protein_id="HFO5819665.1"
                     /translation="MGWSRTKSLSTELPHKPSMMLWFMTGAFMAVVGALLFIIRASEY
                     VKALNDFSIWWLALTPPGGWFFLFCLRHWQWSNQMDEHLFFKKEGEYAQKQWESWAER
                     YLVITASCVYLPDKITVATLCDELPLQYGLVKKIDYLSDSGHKVEASLRVLLREIMDK
                     FCQLPAVLPVNVTLITDQPDSEIRSAFVSAWEVLFPQRVVPDNIEVTPDFSMGWVDER
                     LKQPVLTVDLILVIQLNGGNAYSDGLAALLLTSDDVAQKYNLPHPARLLRPMSLDINK
                     FNDEFTLFLETQTAACRTARVLGDCYHWEKIAAPLMTIGNQYGAGWEPSGRMLLEKWC
                     GIPGPAAPWLLTALAADLVCFGNDSLLTLFSSGEEHFISTVTSGNQNE"
     gene            complement(12095..12361)
                     /locus_tag="ACHKBL_002816"
     CDS             complement(12095..12361)
                     /locus_tag="ACHKBL_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAAR domain-containing protein"
                     /protein_id="HFO5819666.1"
                     /translation="MSKGFVLLGDNTTHGGKVISASSTMIVDGKKVALVGDMVSCPVS
                     GHGTNPIVEGSPEWISDGRAVVIDGCKCLCGCKVISSAPECAIG"
     gene            complement(12392..13069)
                     /gene="tli1"
                     /locus_tag="ACHKBL_002817"
     CDS             complement(12392..13069)
                     /gene="tli1"
                     /locus_tag="ACHKBL_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000196359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T6SS immunity phospholipase A1-binding
                     lipoprotein Tli1-EAEC"
                     /protein_id="HFO5819667.1"
                     /translation="MTRDKIIFIAPLLALLTGCNSVSSQYADSDKTEWGYKKLPYDEW
                     KFLFIYPKALPALATQALMVDGEGYKTTYQYLDTTRPSQVSVGRWNDHLGATESYYNK
                     GKALPLMLRFCWDSIIDKKSYETLIFFKKGTWEQMVTPYTDNRWNETYYRSTMIIGLA
                     PEGKVRVWLGDRGNPVVPQLDAKITTVSGDKMNMCKGVTKHPDGYVYYGDTPDFIKGK
                     IYPYGNW"
     gene            complement(13089..14771)
                     /gene="tle1"
                     /locus_tag="ACHKBL_002818"
     CDS             complement(13089..14771)
                     /gene="tle1"
                     /locus_tag="ACHKBL_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T6SS effector phospholipase Tle1-EAEC"
                     /protein_id="HFO5819668.1"
                     /translation="MTKYQGYDVTDATHKTSIHNDWKVVVAKKKPARGVTLTIGIFFD
                     GTGNNRENTASRLMKFNECSAARQGVNQKDAQSCEDFLEEINKNSISNGSYRGYYSNI
                     HWLNSLYVADQTLLESQVEAQIQTYISGIGTAAGEADSVIGMGLGTSILDIFEGVVTK
                     TDEAMERITQALSEFMGFNLNPDFCIAKIQFDVFGFSRGAAAARHFANRVMEQDPAIA
                     RAIAKGLRGDFYDGKPSGEVRFLGLFDTVAAIGGISNFFDINGRSNPGVKLELRPSVV
                     KKVFQITAMNEYRYNFSLNSIKGMWPELALPGAHSDIGGGYNPVGSPLQENESLFLSC
                     PEFEIVSDDTRETDTRVYRNAEQARKMLMTLPALKHILPHGKLTTKIRSVGVNNSNQR
                     RAGVIQKQVGAAVFFERMAVPNDWANVCLRVMLDAAQEAGVLFEPIRQTNTELQLPSE
                     LIFLADKAIAQGKAVRLGQEPQAFTEEELYIIGKYTHCSANWNIESDGNLWVDPTTGE
                     IFIHRFGPKGNKAFVFPNKPNDRWIRSVWYMDDQQRLNDNAVKNTKVMMSGV"
     gene            complement(14768..17287)
                     /locus_tag="ACHKBL_002819"
     CDS             complement(14768..17287)
                     /locus_tag="ACHKBL_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system Vgr family protein"
                     /protein_id="HFO5819669.1"
                     /translation="MSLTDTLSKTISASLNRYHLIIPSCPSPLDVEHFSGREEMSQTY
                     YYIINFTSTDNDLDAGLLLRKSATFTMGTGGLKEQVSQKVVHGVITDFRRISGSADQA
                     KYQVILEPFIKLLDRQHRSHRFFVNKSVPEVVTEVLQEHGLKGWEFEFRLKKTYPKRE
                     QINQYQESDLQFIQRLLAEVGIFYFFTLQPDTQTEVVHFGDSQAALTFDKTLAINSPS
                     GMNDNRADSVWGLNVTHNVAEASVTTKAYNHREAQYLLQSAPADMTHGDGDGINYGEV
                     YHYHFRHLERGDKIDPVPETANFYARLGHERYLAEQVRITGNSTDATLAPAQVLTITD
                     SLPPTLPALLRNPVLLTCVGFSASRKDALQVVLKGVPYSEVICWRPPLLPRPKVTGTM
                     TARVTSAKEGDIYAWQDASGMYRVKFDADRDDKNPGQESMPVRLAKPYSGDAYGFHFP
                     LIQGTEVAIAFEEGDPDRPYIAHALHDSRHVDHVTDKNGTRNVIRTPANNKLRMEDKR
                     GEEHIKISTEFGGKTQLNLGHNVNASRKLRGEGAELRTDDWISIRGGKGIFISADMQP
                     QAQGKMLDMNEAIRQLEQALSLARSMAKAATAANATQGDISCQQRLNSSLTDLTAPGM
                     LLHAPDGIGMVSARALRIASGSESVGIMSGDNTDISAGQSFTVVAEGAVSLLSRNQGM
                     QLLAAKGRVNIQAQSDDLSMSSQQNLDIQSSEGKVTVSANQELILACGGAYIKLSGGN
                     IELGCPGQILLKSTNMQKMGPTSLDIASVEMPRGFGGGFILTDEAGVPQPSTPYRLTT
                     AEGDILQGITDENGKTAPVNTSIPSVVKVEFGKVKIHGETE"
BASE COUNT         4451 a         4838 c         3904 g         4309 t
ORIGIN      
        1 ctttattttc cttctcagta actgaatatc tttgattaac gccctccgga aatatttata
       61 cccggaaggc tgtccagctt tatgataccc gccagtacag aattaactgc aggctgagca
      121 cagatttctc tgctatttca gtgtcagatt aatactgatt accggaacaa atgggcagat
      181 gtgtaaatcc aggtttgctg gccatataaa gcggcgcaca ggtgggttta cggggttcag
      241 tgaccggtac aacctcattt ttcccggcca ccgacggttg tgacaccttc cggacagccg
      301 ctgatttttt cttctgaacc agcttcgtgg tgaccggtga tttcacctga cgtttggcgg
      361 gtgccacgcc ataaccggaa caaaagcgct tcgactgact cagacttccg tcattgcaga
      421 caaaacgtcc gtctgacgtg cagtgtgaaa taccaccttt agcacccgag cagggttttc
      481 ttccccgcgc agcatctgtc tgaaccgaaa ataatctatg gtcgcccccg tttttgcaac
      541 actgattttt taattaatgg tgggcttgct taaatctatc cggcgtctct gtaggcaagg
      601 atgcctgcgc cccgatgagt ttcgcacctg ataatcctaa aaagcccctc ggcttcagtg
      661 agccatttta tttattaggt ttttaatcag gtcgatttgc cgttcatgtc atcaataatc
      721 agtcttcgca aaaccagaca ggcaactttg tatcttattg tttacaataa gccaattgag
      781 ttatatcagc atttatatca agcctgatat tctttttgct gagtagtgat tacccaggca
      841 attcgagcca gtttatttgc cagagcgcag gcaaccacat tcgaatgctt cttgttgagt
      901 tgctcccgaa cccattcggc aagttttcca tgctggcgtt caagccgcat catgaatgag
      961 cgggcacact gaaccagtaa ccggcgcaaa tttttatcgc ctcgcttact gatacccaac
     1021 agagtcgatt ttcctcccgt gctatactgc cggggaacga gccctgtgga agccgcaaaa
     1081 tctctgctgc atgaaaactg tttcccgtca ccaggttgcg acgatagtaa gctggcagta
     1141 atcggcccaa caccaggaat tgtcataatg cgctgagcgg tatcatcagc gttgattgac
     1201 tgacttaatt ctgattccag ctctgctatt tgctcaacaa ggtaaaggta gtgggtatgc
     1261 aacctcacca aaaggcggct cagatattcg gggatttcgt gttcagccaa cacgagagac
     1321 aaccgtttta ttacagcatc acctgtcggc aggctgattc caaattccag caagaaaccg
     1381 tggatctggt tggttgtttt caccttatct ctgatgagtg attctcggac ccggtgtaac
     1441 gccctcatgg cctgcagggg ctcagttctt ggctggacaa aacgcattga tggtcgtgaa
     1501 gccgcttcgc atatggcttc tgcatcaaca aaatcattct tattactttt gacaaacggg
     1561 cgaacgaact gtggagaaat tagcttagtt tcatgcccaa aatcagagat acgacgagcc
     1621 ataaagtgtg cacccgcaca ggcttccatt acaacaactg agggcggaca tgtcgccaga
     1681 aactgcatca gtttagtacg ggtaaatttt ttacgcagaa gagccttacc aaacttgtct
     1741 tgacaatgaa tgtggaagga gtgctttccg agatcgatac caataagtgt gatgttctgc
     1801 atgatggtct ctccgttaaa gggattcacc ttaacagcgt actgctgtta aggtgggggt
     1861 gaccatctca ttagtcccac gaacagaaaa cagataattt tcttcattta aaaactcata
     1921 cattgttatt gctgatatgc cagagttatc aggatgatga aacattccgc cagcgttgtc
     1981 ataagccgga ataatgtcac attcacacct gacatatatc catcactgtt tatttctgtt
     2041 ctgccctccc tgctccggct cacgtctgaa cgtaccgctg ctgtttcaga tggcgcaaca
     2101 acaccctgcc ctccggtata aatgttgttc cgtaacgaac cagcccggca tctttaagtt
     2161 ctgcatcaat gacataatgc aactcaccag gctgagtctg ctcctgcatc gtcacgttga
     2221 cacgcttaat acgtggctca tacttcagca gaacatctga aagcacacgc atcagttgat
     2281 gcgccgttcc cggcattccc tgcagaacgg ttgtcatatc cggcagacca taatccggca
     2341 gatgtttaag gcttccggcc cgggtattca gaatacgctg catgttatcg agcaccgaaa
     2401 gaataacctg ctcttccgca ctgacctggt ttaactccag cccgccagcg aaattgccat
     2461 agagaatttc ataaagggaa ggacgcggca tcactattta tccttcaccg gtaacagcgt
     2521 cagggtatta ccggatactt caataagacg gggcgtgtcc ggctcaagct catcccggct
     2581 cagcaccacc cgccaggtat ttttcttccg gtcaggctcc agaaacattg ctgccacacc
     2641 ggtaaatttc gccgcatcat ccagaggcat atccacggtt actgagccac ctggctgcat
     2701 ccatacatct ttctgtgcaa tgatatcgcc tgccagaatt tcactgtccc ccgtaaaaag
     2761 cgcctgataa tccgcgctgt caaaattccg gttgtcttta agctgataaa tccgcaccac
     2821 aaccgacagg ggaataccgg cagcactggt gttaatggcc tccctggcac gaatatcaag
     2881 gtgaagtgtt tttatctgtc ggtaaaacag ggatttcgtg gcagataccg taccgtctgc
     2941 cactctttgc gtcaggccac accctgataa cgacagggaa aacaacgcaa taatcagacc
     3001 gtaaccagcc ttaccagcga taatcgccat tttcctgagc ctccttacgt tgataatgtt
     3061 cctgaacccg ctgataacgc cccagacgga tggtcattac gcgtgccgct gaagtcgtta
     3121 tctttgcctg tgttctcatt acagccgtgc gccccagttg cggactaccg gctgccggtc
     3181 ggcaactgag ccggacatcc ggcaacagtg aacgctcaac acaaagttgc agacgtacat
     3241 ccagacggct tcccagataa acgtgcaaca acgccagcaa atcagaatac agctgcccgc
     3301 ccggtaacca gccctgtacc tcgctgcccg tctgtgttga aagctgtaat aacacctgtc
     3361 cgttcacatc cgtagcatga tcgcccatca ccgggcgact cttcagactg accggatgat
     3421 gaacacgcat ggccagcggt gttttcagcg ggatccgtcg tcggtcatga tgccagacac
     3481 gggcctgtgt tccgggagcc agcagcgtta ccagtgatgt cagcccttca gctgttcttc
     3541 cgggtaacaa catcaggggc aataacgcca gaaaacgcga taccggcgtg gcaatattct
     3601 gtgcacatcc ggggatcccc agtctggcca gaccgagcag atactgggat gttttatcct
     3661 gcccccccgc ctcaaaggtg gccgggtacg aatatttgcg ccagatacgg taatactggg
     3721 tgatcagacg atgactgaat atatcgagaa aatccgctgc ggcttcatgg ccttcccgct
     3781 gctgtgcaat atcactgata tagtgagccg gcaacggaga cgtgaccccg taaagtccca
     3841 taaacgtcac atgtacagtg ggtggcagat gggattcttc aggaattaca gcatctttta
     3901 tttcactcgc aggaaacccc attcccgtgt atggacaaaa gcgaaccgct tcctgacggg
     3961 cctgccagcc actgccaatg ggaggctgat ctggctggct ttgttccagt aactgacaga
     4021 agcgataaaa attaatatac ggaagctgtt tccggtaacg ggtaattaac cgggcaggcg
     4081 cggattgtga ttttctttcc accggatgca ttttccttca ggctgaacaa tcagtgtgag
     4141 ctgattgaac tgattcatat cggcgtacag ggccaggaaa cggttgagca tttcgccaaa
     4201 caggtgaata tctccctctc cggcaaaccc gttgccatca agagttacct ccacatcaag
     4261 cccccggagc agaaaaccct tttcaaaacg ctgaatacga tgatgctgta cagccagaat
     4321 ggcatcaagc cgacggtgat taagttcatc gtcccgccag ttataaagcg ccagcgtgcc
     4381 acgcagcact tccgcactgc tcagcatgtt aagaaatcct gtccccagat gactcatcac
     4441 ccgccagtga aaacgatctt ctgtcggcgg ataaaccggc agcgtgggtt tacaaagatt
     4501 acatacactg atcggggtct gcagtacctg ttcacaccga tcaagcagcg tactctgcaa
     4561 cgcacgacgg ggaagctgac cattggtacc tgtaatacgt aatgaaagcg tttcccgctc
     4621 cggcatccga tccgcctccc accgctgacc gccaaggata agccatgtgt catacatccc
     4681 cgtgacaccc cgcttcaccc gggtatgata ataacgttcg ggtgcatcat ggcgcagcat
     4741 tcccccacga tgacggaagc tggtaaaagg cacgtactct gcactgccgg ttcgcccgga
     4801 gcccgccacc gcatccactg aatatatctc cgtatatcca tcctgcagac gcttcgggcg
     4861 cagcagatat tcgctctcca gcccattgat gatgagtgga tcggcttcaa gcgtaaaaag
     4921 gttaatcacc ggcacacaat gcagacacag ggcatcatct gtcacaggta aatctgccgg
     4981 ccaggactga ctcagcacca cctcaatatc aaatccggaa atacctgccg gcaaagaaat
     5041 attctccagt ccgttcagat gaacaaacat aaatttttcc cgaaaggtga aatattccag
     5101 caataactga tacccactga aagcactgtc acctttcggc cacagccgat cttcttccgc
     5161 aaaaccaccg ggtgaaaacc atccgtcaat gcggatccgc tcatcatttc ccggcagcct
     5221 cagatacagc gcagccagac gctttgtcat catcagatga agggtgctgc tgaccggggc
     5281 ttctgctgcc agatacaggc tcagacgatg caaatcgacc tgagaccagt cagccagttc
     5341 actgcaggca aaacctattt tcagcactga gcgcccgtcc ggctccgtag tcataacgac
     5401 cttctccact gccagtggat tctgtggtat ggcccgggtg gtacggtaac gacagactgt
     5461 atttccgggg ccaacagggc gggaagtcac ttccagtccg gctggtacgg tttcagccat
     5521 tttcatgacg gataaccggg gagtcagtgc caccaccgac agggacggga tagtacgcag
     5581 gtaatgaggc cagagcatac tgaccagact ttcagtaagc tccggcaaat catcatcgat
     5641 tttctggcgc agccgtccca tggaaaaggc gaaaccttca aacaggcgtt ccacgcaggg
     5701 atcaggcgta cccgctttat cgagatccag catcgccgcc cgatcgggat gagcctgtgc
     5761 aaacgccttt ccggcttcac gcagataacg catttcagcg tcaaaataac gcagggttaa
     5821 atcgtccatt atcttctcag aataaagcta actgttccgg agactcgcgc tctgacttca
     5881 gccatatttc aaacaatcgc ccgaactgaa ctgcaggcag gatttcatta atcagattac
     5941 acatgaatat aattcagaca tctcatgaac ttcccgaatt gcacaacaca gcagctctgg
     6001 cagcatcaat ggcgatgagc ccggcaagaa gatgttccat atcaggctgc agacgcaccc
     6061 tgtcactttc agtttttgcg gacttcatac gtaacagttt caggcggcgg gcttttacct
     6121 caaataccag ttcaggttcc cattgtgaca gggtcagccg ggttgcacgt tcatccagct
     6181 ccacaagcag atgcagggca aggtcatttt ttccagtctg ttcggcaaca cgggccatca
     6241 acaggcgcag tagccagttg ctgcggtcag actgaatacc gggacgtgcc tgtaaccagc
     6301 ttaaagcggc ttctgttcct tcattatccg caatctgcag ggcttccggc tcaagagata
     6361 aaatgtcgtt atctccggga gctgccgtta tcgttacaga aggttcttcc gcccagcgtt
     6421 caccacactc catcacctgc tgctgtatcc agctcagcgt cacgtcatcg gcaaacggca
     6481 tgccgtcgtt aaaagccagt gtctcaagcc ccggcaggcg cagaagcaat cctttcaggt
     6541 catactgaat aatggcagca taattttctt ttccggtctg caacagcgcc tgatgaatat
     6601 accactgcag gtccagccac agatgactgg ctccccgggc aaacatatcg tcacactgtt
     6661 caagcagtga caaccagttc tgctgcagat acaaccgttt cagtgacgca cgccggtccg
     6721 gccccggtgg ggcaatacgg gtccgtccat cagcagataa cggcggtaac tgatgcagcg
     6781 tgtcatgccg gacgcttttc atcagtcgat gagccgccag ccagccctca ggctggtcac
     6841 gcagatattt tgccagaaca cgggcctgtg acagcagttc ctgtccggag gtgatggcag
     6901 ataatgccat gacgctgccg gatgttaccg tcggtgccgg cgcggcagcc tcctgaggca
     6961 ccactgcatc aacaccaccg gccttcatca ggcgattttc aagtgcctga tataacccgt
     7021 tcagcaccgg ggcggaagcc tcatccgtaa aggtctgttc tgccagccac agcgccccgg
     7081 caatgcgcag ggtatccgcc ttcaccacct ccggatacag agacagactg tccagtatgc
     7141 gggcgctgca taaccactcc agcgccgcct tcctggcccg tgaccgttgt ggatgcaggt
     7201 gctctccaaa acgctgcaac aaaccggtca gcagctccag accgtcggcc agcccttttt
     7261 caccatcctg ccgcagacgg gcccagatat accaggtcgc cacccggata tttttggttc
     7321 tggtggtcag taatttttct gcaagctggc tgacaattcc ggcatcggct ccggagagtt
     7381 tattgacctc ttcacgcatc tgctgaaaat catcatcgta ccccgcatca tcacctgcgg
     7441 gattatctgg cgtcacggga gcaagccatt tatcccacag cgcaatacgc acctgagccg
     7501 gttcagccac atcccggggt gtcgtctggc atgcactgag gagcgaatga atggaagcca
     7561 tcagtcagtc tcctccacgg aatccccggc atcttcgtca ttgcctgtaa acgctgacgt
     7621 accggagagt tcgaaaaccg tttccggcag aacaaaattg cggagtttca gcaatacaag
     7681 cggcccttcc cccgcttcag ttcgcagtgt gtaattcagc atacgaccgt cctgcgcctg
     7741 ccagctcagg ctccagccac tggcaacgcc cggtgcggct ttccgtcggg ccatctcaag
     7801 cagacgtatc agcccccagc ttcccggcag gtctgcatac tgacgggtac cggcctgagt
     7861 gcttacccag cttaaactgg cgccaggtgc ttccgtatca gccggccagg taaatcgctt
     7921 ccataccggc atctgattaa cataaatcag tttctgatta tcggttatca gcgtcgtctg
     7981 catcacaccg gcagcagttc ccgggcgcag ctcaaaatgc agccccgcgt tacctgtggt
     8041 gaacgccaca tccgctattt cactcagggt attgatggct ttcagaaacg cagggttgaa
     8101 tgtcagtccc cgggtattaa tggtatcagg tacccagcga ctgccttcac tgtgcaacac
     8161 accactcagg ttattctgca ggaaacgggc aatacgtccc gtgtccgtat tcaggtattt
     8221 tgccagcagg ggaagcgaag catcgctgct gacattttta aacggataac gcccggaaaa
     8281 tgcattattc cagccatcca ccaccgcagt acgccaccgg gcattcagac tttctgccgc
     8341 gggcgtaaga acctgctgcc acgcctgctc catcgggcga acaaagaccg tctgtccgaa
     8401 gccataccac tcctgtccga gtccggcagc ggtcaggcta ccgtaatccc gggtatcggt
     8461 taaatcaacc gatttcccct gcaatactgt ctgtgccagc atctgcatca tggcctgcgg
     8521 gtcagaagag ccggcaatct gttgcagacg cagacgaacc tgtgtcaccc gggtcagata
     8581 tgtctgcaga ttcagcatat ccgcactgtt gttctgatta tccatcagtg caagcaccgg
     8641 cccgaaagtc gtcgccagag gaccgtgaag ccggctttcc ggtactgcca ctggctgttt
     8701 ttcctgtgaa agcaggtttc tggcagattt aaccagtgaa tcagtaaccg cttcccgggg
     8761 ctggccagta cgcccctgaa ccgccagcgt attcatcaga gcaaccaggg gggactggcg
     8821 cacatcagcc atcagcgtca gttgctctgt cacatcagac agcgtctgag ctttacgcaa
     8881 gtgcagactg ttcagaaaat tcagccaggc attaccaaaa tcagcaaaat agcgcgttgt
     8941 caggcgctgt ctgagtgctt ccggcgatac cggtgacggt gccttctgcc tgccatccgt
     9001 cagtacccag tccatttctt cacgacgctc attaatcacg gtgtctatcg acggcaatac
     9061 ggcctcttcc caggcctggc gggtaaacat accgggaacc acttcatccg taaaaaagag
     9121 gcgggagaca tcagtatcac cggtcatatc cgtcagacgc atatccgcaa actgatgtgc
     9181 cacccgtgcc agcattttct gatacagcat ggcatcgctg ttctgtgttc ccagatggcg
     9241 aagcagcaga gttctgctct ggctgaccag ctcttcatca gccgtcagct tccactgagg
     9301 atgagtaata atcccggagg cataatacgc cagcaacgtc gggccattag cctgccagaa
     9361 aacggcactc acaccgtcac gctgtggcca ttcccgcatg agcgtacgac tgaacctggc
     9421 gggctccata tgttgcggtt gcgccagcat cagatacagt ctgagctggt catatgcggc
     9481 ttttgccatt ttcccccgct gcgggctgtc cggaggaaga cgaataaatg tcctgagctg
     9541 ctgctcaaga tgagcagcag cggcatcacg cagtaaaaga tgtgcatttt gtgcataaag
     9601 gggcattacg accgcgagca ggtctgcatt ctgattcata ccggcccgca ggtaccatgg
     9661 cgccccgtgc tcctcccggt actgaaggcg ggccagggat ttctgcagat tcagcaacgc
     9721 ctgcagacgt tctgcaggag acagttgctg gttctgagcc ggacgaatct gttcctgtac
     9781 ttcagcaacc agactccggt ttgccagaaa ggaaaccacc attcctgttc cctgagccat
     9841 catcagtatg gccgccataa cggccagact tctgcgccag ttatgacctg tgcgggaagg
     9901 ctgtagccgg acaggtaatt gctgcactgt ctccggcaac gcctcccagc ggttatccat
     9961 tcgccagcga tgtctgacac ttcgttcacc gccgacagtt gccggactga aaacaatacc
    10021 cgccagcaac aactgacggt atggacggag taatacggat aatggctcca ccacagcatc
    10081 aatatttcgt ctgaatcgct ctgccagcga caacaaaaaa taataccgag gggcacagca
    10141 tatctgctgt atcccctgag caacaagccc ggggagcatg gcctgtagtt gtgctttcag
    10201 tttgtcagaa ctacattctg ctggcagaag gcatcccacg ggctgagtaa tacgccctcg
    10261 ctcatccgga ctctcctgca gtgaccagac atacagcggc agacgccagc caaggcgctc
    10321 acaacatgaa ctgaccgcac gggaaaccct gtcaagggca tcttctgtca gtggtgcgga
    10381 taatgtttca ctcagtccgg aagttaccca gacaatgcca tcagcaggcc ggtaacgcag
    10441 acggcgtaac gcagccagcc agtctgcgtt ttccggctgc gaggggtctc ctccccagag
    10501 aagcaatgtc ccctgctctt cctgccagaa ctgttctgtc aggcccgggg tcagcaactc
    10561 aacctcagag gcagtacctg ttatcaacag aatgcgtatt ttacgtcccc agaatctgcc
    10621 atgctggcgc cgcagagtat gacgaatatc ctgaacagta acatagcgcg gtgacgtctg
    10681 agccagtcgc tcttcatcgt caggtaacct ttgctcctta cgggcccgac gtcgatatgt
    10741 cagttcctga acatattttt tcatcacagg caggtaacag acaaagagca accccagact
    10801 cagcaatatc agcagtagta tttgatcccg gcgtgtttct gcccccagaa atgctccata
    10861 tcgggttatc agccaccaga gcgcagccac accaataaat acaatccccg gtcgtccaaa
    10921 gcgaccagac agacaggcca gtttattcat tctggtttcc tgatgtaaca gtactgataa
    10981 aatgttcttc tccggatgag aataaagtca ggagagaatc attaccaaaa cagaccagat
    11041 cggctgccag agcagtaagc agccacggag cagcaggtcc cgggataccg caccattttt
    11101 ccagtaacat acgtccggac ggttcccatc cggcaccata ctggtttcca atagtcatca
    11161 gcggtgcagc aatcttttcc cagtgataac agtcccccag tacacgagcg gtacgacagg
    11221 cagctgtctg ggtttcaagg aaaagtgtga attcatcatt aaacttgttg atatccagcg
    11281 acatcgggcg tagcagacgg gctggatgcg gcaggttata tttttgcgca acatcatcgc
    11341 tggtcaacaa aagtgcagcc agtccgtcag agtatgcatt tcccccatta agctgtatta
    11401 ccagtatcaa atccacagtc agcactggtt gtttcagacg ctcatcaacc caccccatgg
    11461 aaaagtcggg agtaacctcg atattgtcag gaacaacacg ttggggaaat aacacttccc
    11521 atgcagaaac aaaagcagat ctgatttcag aatcaggctg atcggttatc agcgtcacat
    11581 ttacagggag aacggcaggt aactgacaaa atttatccat tatctcccta agcaagacac
    11641 gtaaagaggc ttcaacctta tgcccactgt cagaaagata atctattttc tttacaaggc
    11701 cgtactgcag aggcaattca tcacaaagag tagctaccgt tattttatca ggcaaataaa
    11761 cacaactggc agtaataaca agataccttt cggcccagga ttcccactgt ttctgagcat
    11821 actccccctc ttttttaaag aacaaatgtt catccatctg gttgctccac tgccagtgtc
    11881 gcaggcaaaa caggaaaaac cagccccccg gaggagtcaa tgcaagccac cagatactga
    11941 aatcatttaa tgctttcaca tattctgatg cccggataat aaacaacaga gcaccaacaa
    12001 cagccataaa tgcccctgtc ataaaccaca acatcattga tggtttatga ggtaattctg
    12061 ttgataatga ttttgttctg ctccatccca tgggttatcc tattgcacat tcaggtgcac
    12121 tggatataac cttacaccca cacagacatt tacatccatc aatcacaaca gctcttccat
    12181 cagatatcca ttcaggagag ccctccacaa taggattagt accatgtccg gaaacaggac
    12241 aactaaccat atctccgaca agagctactt ttttaccatc gacaatcatg gttgaagatg
    12301 ccgaaataac tttaccacca tgtgtggtat tatcaccaag caatacaaaa cctttagaca
    12361 taaaactctc aaaaataaat tttactttga gtcaccagtt accgtagggg taaattttcc
    12421 ctttgataaa atctggtgta tcaccgtaat aaacatatcc atctggatgt ttagtaaccc
    12481 ccttacacat attcatttta tcacccgata ctgtagtaat tttagcatcc aactgaggta
    12541 ccaccggatt accgcgatct cccagccaga ctcttacctt cccttcaggt gccagaccaa
    12601 taatcatcgt tgaacgataa taggtttcgt tccagcggtt gtctgtgtaa ggcgtaacca
    12661 tctgctccca ggtccctttt ttaaaaaaaa tcagagtttc ataactcttt ttatcaatta
    12721 ttgaatccca gcaaaaacgt aacatcagag gtaatgcttt ccccttgtta taatacgact
    12781 cagtcgcgcc cagatggtca ttccagcgcc ctacactgac ctgactcggt cttgtggtat
    12841 caagatactg ataagtcgtc ttataacctt ccccgtccac catcaatgcc tgagtggcca
    12901 gcgcaggcag agcctttgga taaataaaga gaaatttcca ttcatcatag ggtaattttt
    12961 tataacccca ttcagtctta tccgaatcag catactgtga gctaaccgaa ttacacccag
    13021 ttaacaatgc cagtaatggt gcgataaaaa tgattttatc tctggtcata tattgccacc
    13081 ctgtggtgtt aaacaccaga catcataact ttagtatttt ttactgcgtt atcattcaat
    13141 cgctgctgat catccatgta ccatacagaa cgaatccagc gatcatttgg tttattcgga
    13201 aaaacgaacg ccttattacc tttaggacca aagcgatgga taaaaatctc tcccgttgta
    13261 ggatccaccc ataaattacc atcagactca atattccagt tggcagaaca atgggtgtat
    13321 tttccgataa tatacaactc ttcctcagta aaagcctgag gctcttgccc caaacgtacc
    13381 gcttttccct gagcaatggc tttgtcagca agaaatatta gttcagatgg aagttgtaac
    13441 tcagtatttg tttgtcgaat tggttcaaac aatacccctg cttcctgagc agcatccagc
    13501 atcacacgca ggcaaacatt agcccagtca ttaggtactg ccatacgctc aaaaaatact
    13561 gcggctccca cctgcttttg tatcacacca gcccgacgtt gattgctgtt gttcacacct
    13621 acggaacgaa ttttggtggt tagttttcca tgaggcaaaa tatgttttaa ggctggcaga
    13681 gtcatcaaca tttttctggc ttgctctgca ttacggtata ctcgcgtatc cgtttctctg
    13741 gtatcgtcac tgacaatttc aaattccggg caactcagaa acagactttc attttcctgc
    13801 agaggtgaac ctaccggatt gtaaccacct ccaatatctg aatgcgcccc tggtaaagcc
    13861 agttcgggcc acataccttt aatactgttc aggctgaaat tatatcggta ttcattcatt
    13921 gcagtaatct ggaaaacttt cttcacgact gaaggacgca attccagctt tactcccggg
    13981 ttgctacgtc cattaatatc gaagaaatta ctgattccac caatagctgc gacagtatca
    14041 aaaagcccca gaaaacggac ttcaccagaa ggtttaccat cataaaaatc gcctctgagc
    14101 cctttagcaa tagctctggc tatagcagga tcctgttcca taacccgatt ggcaaagtgc
    14161 cttgccgccg ctgctccccg gctaaaacca aaaacatcaa actggatttt tgcgatacaa
    14221 aaatcaggat ttaaattgaa ccccataaat tctgataacg cctgggtgat tcgttccatt
    14281 gcctcgtcgg ttttcgtcac cacaccttca aatatatcca gaatactggt gcctaatccc
    14341 atgccaatga ctgaatctgc ttcaccagcc gcagtcccta tcccggaaat gtaagtctga
    14401 atttgtgctt caacctgact ttccaacaaa gtctgatctg ctacgtaaag tgaatttagc
    14461 cagtgaatat tggaataata tcctcgataa ctgccattcg agatactgtt cttgttaatt
    14521 tcttcaagaa agtcctcaca gctttgtgca tctttttgat ttaccccctg tctggctgca
    14581 ctacactcat taaatttcat caggcgagat gctgtattct cccgattgtt tccggtacca
    14641 tcgaaaaata taccaatggt taaagtcaca ccccgcgccg gttttttctt tgcaacaaca
    14701 actttccagt cattatgaat actggtttta tgggtggcat ctgttacgtc atatccctga
    14761 tattttgtca ttctgtttct ccatgaattt ttaccttccc aaactcaact tttactactg
    14821 acggaatgga ggtgttgact ggcgctgttt taccattttc atcggtgatc ccttgtagaa
    14881 tatcaccttc agccgttgtt aatcgataag gcgtcgaagg ttgcggcact cccgcttcat
    14941 cggtaagaat aaaaccgcca ccaaaccctc gcggcatttc aacagacgca atatcaagac
    15001 ttgttggtcc catcttctgc atattcgtgc tttttaacag gatctgtccc gggcatccca
    15061 gctcaatatt tccaccgctt agtttgatat aagctccacc acatgccagt atcagttcct
    15121 gattagcaga aacagtcact ttgccctcag aactttgaat atcaagattc tgttgggaac
    15181 tcattgacaa atcgtcactt tgagcctgaa tatttacccg cccttttgct gctaacagtt
    15241 gcatcccctg gtttctgctc aacaggctaa cagcaccttc tgctacaact gtaaatgact
    15301 gcccggcact aatatcagtg ttatctcccg acattatgcc cacgctttca ctacccgaag
    15361 caattcgtaa cgcccgtgca ctaaccatgc caatcccatc aggagcatgc aacagcattc
    15421 ccggcgcagt gagatctgtt agcgatgaat tcaaccgttg ctgacaactt atatctccct
    15481 gagtagcatt tgcagcagtt gcggccttcg ccatacttcg ggccagagat aatgcctgct
    15541 ccagttggcg gatagcttca ttcatatcca gcatctttcc ttgtgcctga ggctgcatat
    15601 ctgcactgat aaatattcct tttcctcccc ggatacttat ccagtcatca gtcctcagct
    15661 cagcgccttc tcccctgagt ttccgggagg cattaacgtt atgaccaaga ttaagttgtg
    15721 tcttaccgcc aaactcggta ctgatcttga tatgctcctc accgcgtttg tcctccatcc
    15781 gcaacttgtt atttgccggc gtacggataa cattgcgcgt accgttctta tcggtaacgt
    15841 gatcaacatg acgtgaatca tgcagggcat gcgcgatata ggggcgatcc ggatcacctt
    15901 cctcaaaggc aatggccact tccgttccct gaatcagcgg aaaatgaaaa ccataagcat
    15961 caccactgta gggtttggcc agacgtaccg gcatgctttc ctgtcccgga tttttatcat
    16021 cacggtcagc atcaaatttc acccggtaca tacctgatgc atcctgccag gcgtagatat
    16081 ccccttcttt tgcactggtc acacgggccg tcatggtacc ggtaactttc ggacgcggca
    16141 gtaaaggagg gcgccagcag ataacttcac tgtaaggaac gcccttcagt acaacctgca
    16201 gggcatcctt gcgacttgca gaaaaaccga cacaggtaag caacaccgga ttgcgcagca
    16261 gcgcaggcaa cgtcggcgga agactgtcgg tgatagtcag aacctgagca ggtgccagcg
    16321 tggcgtctgt actgttgccg gttatccgca cctgctccgc cagataacgt tcatgaccaa
    16381 ggcgggcata aaaattggcc gtctccggca ccgggtcgat tttatccccc cgttccagat
    16441 gacggaaatg gtagtggtac acctcaccat agttaattcc gtcaccatca ccatgcgtca
    16501 tgtctgccgg ggcggactgc aacagatatt gtgcttcacg atggttataa gcctttgtgg
    16561 tgacactggc ctctgccaca ttgtgcgtca cattcagccc ccacactgaa tccgctctgt
    16621 tatcattcat tccggacgga ctgtttatcg ccagcgtctt atcaaacgtc agcgcagcct
    16681 ggctgtcacc aaaatgcact acttccgtct gcgtgtccgg ttgcagggtg aaaaaataaa
    16741 aaatccccac ttcagccagc aaacgctgaa taaattgcag gtcactttcc tgatactggt
    16801 taatctgttc acgcttcgga tacgtttttt tgagacggaa ctcaaactcc cagcctttca
    16861 ggccgtgctc ctggagaacc tctgtcacca cctccggcac cgatttgtta acgaaaaatc
    16921 ggtgagaacg atgctgcctg tccagtagtt taataaatgg ttccagtatc acctggtatt
    16981 ttgcctggtc ggcggagccc gatatacggc gaaaatctgt aatgacacca tgtactactt
    17041 tctggctcac ctgctcctta agccctcccg tacccattgt aaatgtggct gatttacgca
    17101 gcagcagccc ggcatccagg tcgttgtcag tactggtaaa attgatgatg taataatagg
    17161 tctgactcat ctcctccctg ccgctgaaat gctcaacatc cagtggtgac gggcaggagg
    17221 gaataattaa atggtaacga ttcagggaag cagagatagt ttttgaaagt gtatctgtca
    17281 gactcatatt gttaactcca tgtctgtacc ggtattgaac tgcaattata taatgatgac
    17341 cgttaagctt tattttcaga gagaattact gataatcatc ccattcatcg taaatatatg
    17401 catatgcaaa tcgatacatc agataaccga caaaaaataa cgcaacagag agccctcccc
    17461 acagatattt aaattcagca gatgagagaa aaaagaacca ga
//
LOCUS       DAZFLD010000102        17436 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.103, whole genome shotgun sequence.
ACCESSION   DAZFLD010000102 DAZFLD010000000
VERSION     DAZFLD010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17436
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.103"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(180..911)
                     /gene="rlmB"
                     /locus_tag="ACHKBL_002820"
     CDS             complement(180..911)
                     /gene="rlmB"
                     /locus_tag="ACHKBL_002820"
                     /EC_number="2.1.1.185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HFO5819670.1"
                     /translation="MSEMIYGIHAVQALLERAPERFQEVFILKGREDKRLLPLIHALE
                     SQGVVIQLANRQYLDEKSDGAVHQGIIARVKPGRQYQENDLPDLIASLDQPFLLILDG
                     VTDPHNLGACLRSADAAGVHAVIVPKDRSAQLNATAKKVACGAAESVPLIRVTNLART
                     MRMLQEENIWIVGTAGEADHTLYQSKMTGRLALVMGAEGEGMRRLTREHCDELISIPM
                     AGSVSSLNVSVATGICLFEAVRQRS"
     gene            complement(1002..3443)
                     /gene="rnr"
                     /locus_tag="ACHKBL_002821"
     CDS             complement(1002..3443)
                     /gene="rnr"
                     /locus_tag="ACHKBL_002821"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HFO5819671.1"
                     /translation="MSQDPFQEREAEKYANPIPSREFILEHLTKREKPASRDELAVEL
                     HIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPERLDLVKGTVIGHRDGYGFLRVE
                     GRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEA
                     GVGFVVPDDSRLSFDILIPPDQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNM
                     GTGMAVDIALRTHEIPYIWPQAVEQQVAGLKEEVPEEAKAGRVDLRDLPLVTIDGEDA
                     RDFDDAVYCEKKRGGGWRLWVAIADVSYYVRPPTPLDREARNRGTSVYFPSQVIPMLP
                     EVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQG
                     DQDLREQYAPLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAERRIERIEQT
                     QRNDAHKLIEECMILANISAARFVEKAKEPALFRIHDKPSTEAITSFRSVLAELGLEL
                     PGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQSYAHF
                     TSPIRRYPDLTLHRAIKYLLAKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRAD
                     EATRDVADWLKCDFMLDQVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYY
                     RFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKIDFSLISSERAPRNVGKTARE
                     KAKKGDAGKKGGKRRQVGKKVNFEPDSAFRGEKKPKPKAAKKDARKAKKPSAKTQKIA
                     AATKAKRAAKKKVAE"
     gene            complement(3482..3907)
                     /gene="nsrR"
                     /locus_tag="ACHKBL_002822"
     CDS             complement(3482..3907)
                     /gene="nsrR"
                     /locus_tag="ACHKBL_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing transcriptional repressor
                     NsrR"
                     /protein_id="HFO5819672.1"
                     /translation="MQLTSFTDYGLRALIYMASLPEGRMTSISEVTDVYGVSRNHMVK
                     IINQLSRAGYVTAVRGKNGGIRLGKPASAIRIGDVVRELEPLSLVNCSSEFCHITPAC
                     RLKQALSKAVQSFLTELDNYTLADLVEENQPLYKLLLVE"
     gene            complement(4112..5410)
                     /gene="purA"
                     /locus_tag="ACHKBL_002823"
     CDS             complement(4112..5410)
                     /gene="purA"
                     /locus_tag="ACHKBL_002823"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HFO5819673.1"
                     /translation="MGNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTL
                     VINGEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEMKELEDRGIPVRERLLLSE
                     ACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAE
                     KLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDF
                     VMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGA
                     GPFPTELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLD
                     VLDGLKEVKLCVAYRMPDGREVTTTPLAADDWKGVEPIYETMPGWSESTFGVKDRSGL
                     PQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA"
     gene            complement(5514..5711)
                     /gene="yjeT"
                     /locus_tag="ACHKBL_002824"
     CDS             complement(5514..5711)
                     /gene="yjeT"
                     /locus_tag="ACHKBL_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HFO5819674.1"
                     /translation="MNSTIWLALALVLVLEGLGPMLYPKAWKKMISAMTNLPDNILRR
                     FGGGLVVAGVVVYYMLRKTIG"
     gene            complement(5793..6797)
                     /gene="hflC"
                     /locus_tag="ACHKBL_002825"
     CDS             complement(5793..6797)
                     /gene="hflC"
                     /locus_tag="ACHKBL_002825"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HFO5819675.1"
                     /translation="MRKSVIAIIIIVLVVLYMSVFVVKEGERGITLRFGKVLRDDDNK
                     PLVYEPGLHFKIPFIETVKMLDARIQTMDNQADRFVTKEKKDLIVDSYIKWRISDFSR
                     YYLATGGGDISQAEVLLKRKFSDRLRSEIGRLDVKDIVTDSRGRLTLEVRDALNSGSA
                     GTEDEVTTPAADNAIAEAAERVTAETKGKVPVINPNSMAALGIEVVDVRIKQINLPTE
                     VSEAIYNRMRAEREAVARRHRSQGQEEAEKLRATADYEVTRTLAEAERQGRIMRGEGD
                     AEAAKLFADAFSKDPDFYAFIRSLRAYENSFSGNQDVMVMSPDSDFFRYMKTPTSATR
                     "
     gene            complement(6800..8059)
                     /gene="hflK"
                     /locus_tag="ACHKBL_002826"
     CDS             complement(6800..8059)
                     /gene="hflK"
                     /locus_tag="ACHKBL_002826"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HFO5819676.1"
                     /translation="MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDI
                     FRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKE
                     AERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
                     MNVQYRVTDPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQR
                     ELEETIRPYDMGITLLDVNFQAARPPEEVKAAFDDAIAARENEQQYIREAEAYTNEVQ
                     PRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEITRERLYIETMEKV
                     LGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGASNLLRLPPASSSTTSGASN
                     TSSTSQGDIMDQRRANAQRNDYQRQGE"
     gene            complement(8145..9425)
                     /gene="hflX"
                     /locus_tag="ACHKBL_002827"
     CDS             complement(8145..9425)
                     /gene="hflX"
                     /locus_tag="ACHKBL_002827"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome rescue GTPase HflX"
                     /protein_id="HFO5819677.1"
                     /translation="MFDRYDAGEQAVLVHIYFTQDKDMEDLQEFESLVSSAGVEALQV
                     ITGSRKAPHPKYFVGEGKAVEIAEAVKATGASVVLFDHALSPAQERNLERLCECRVID
                     RTGLILDIFAQRARTHEGKLQVELAQLRHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRNRIVQIQSRLERVEKQREQGRQSRIKADVPTVSLVGYTNAGKSTLFNRIT
                     EARVYAADQLFATLDPTLRRIDVADVGETVLADTVGFIRHLPHDLVAAFKATLQETRQ
                     ATLLLHVIDAADVRVQENIEAVNTVLEEIDAHEIPTLLVMNKIDMLEDFEPRIDRDEE
                     NKPIRVWLSAQTGEGIPQLFQALTERLSGEVAQHTLRLPPQEGRLRSRFYQLQAIEKE
                     WMEEDGSVSLQVRMPIVDWRRLCKQEPALIDYLI"
     gene            complement(9502..9810)
                     /gene="hfq"
                     /locus_tag="ACHKBL_002828"
     CDS             complement(9502..9810)
                     /gene="hfq"
                     /locus_tag="ACHKBL_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HFO5819678.1"
                     /translation="MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGTSSNYHHGSSAQNTSAQQDSEETE
                     "
     gene            complement(9896..10846)
                     /gene="miaA"
                     /locus_tag="ACHKBL_002829"
     CDS             complement(9896..10846)
                     /gene="miaA"
                     /locus_tag="ACHKBL_002829"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HFO5819679.1"
                     /translation="MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSA
                     LIYKGMDIGTAKPNAEELLAAPHRLLDIRDPSQAYSAADFRRDALAEMADITAAGRIP
                     LLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAAEQGWESLHRQLQEVDPVAAARI
                     HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRF
                     HQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLEGEISYDEMVYRGVCATR
                     QLAKRQITWLRGWEGVHWLDSEKPEQARDEVLQVVGAIAG"
     gene            complement(10839..12686)
                     /gene="mutL"
                     /locus_tag="ACHKBL_002830"
     CDS             complement(10839..12686)
                     /gene="mutL"
                     /locus_tag="ACHKBL_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HFO5819680.1"
                     /translation="MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDI
                     DIERGGAKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIISLGFRGEALASI
                     SSVSRLTLTSRTVEQQEAWQAYAEGRDMDVTVKPAAHPVGTTLEVLDLFYNTPARRKF
                     LRTEKTEFNHIDEIIRRIALARFDVTINLSHNGKMVRQYRAVPEGGQKERRLGAICGT
                     AFLEQALAIEWQHGDLTLRGWVADPNHTTPALAEIQYCYVNGRMMRDRLINHAIRQAC
                     EDKLGADQQPAFVLYLEIDPHQVDVNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQL
                     ETPLPLDDEPQPAPRAIPENRVAAGRNHFAEPAVREPVAPLYTPAPASGSRPAAPWPN
                     AQPGYQKQQGEVYRQLLQTPAPMQKPKAPEPQEPALAANSQSFGRVLTIVHSDCALLE
                     RDGNISLLALPVAERWLRQAQLTPGEAPVCAQPLLIPLRLKVSGEEKSALEKAQSALA
                     ELGIDFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQSVFEPGNIAQWIARNLM
                     SEHAQWSMAQAITLLADVERLCPQLVKTPPGGLLQSVDLHPAIKALKDE"
     gene            complement(12696..14033)
                     /gene="amiB"
                     /locus_tag="ACHKBL_002831"
     CDS             complement(12696..14033)
                     /gene="amiB"
                     /locus_tag="ACHKBL_002831"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiB"
                     /protein_id="HFO5819681.1"
                     /translation="MMYRIRNWLVATLLLLCAPVGAATLSDIQVSNGNQQARITLSFI
                     GDPDYAFSHQSKRTVALDIKQTGVIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDL
                     TENGKTEAVKRQNGSNYTVVFTINADVPPPPPSPPVVAKRVETPAVVAPRVSEPARNP
                     FKTESNRTTGVISSNTVTRPAARATANTGDKIIIAIDAGHGGQDPGAIGPGGTREKNV
                     TIAIARKLRTFLNDDPMFKGVLTRDGDYFISVMGRSDVARKQNANFLVSIHADAAPNR
                     SATGASVWVLSNRRANSEMASWLEQHEKQSELLGGAGDVLANSQSDPYLSQAVLDLQF
                     GHSQRVGYDVATSMISQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEE
                     RLLASDDYQQQLAEAIYKGLRNYFLAHPMQSAPQGATAQTASTVTTPDRTLPN"
     gene            complement(14052..14513)
                     /gene="tsaE"
                     /locus_tag="ACHKBL_002832"
     CDS             complement(14052..14513)
                     /gene="tsaE"
                     /locus_tag="ACHKBL_002832"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HFO5819682.1"
                     /translation="MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTF
                     SRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFAND
                     AICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG"
     gene            complement(14485..16032)
                     /gene="nnr"
                     /locus_tag="ACHKBL_002833"
     CDS             complement(14485..16032)
                     /gene="nnr"
                     /locus_tag="ACHKBL_002833"
                     /EC_number="4.2.1.136"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HFO5819683.1"
                     /translation="MTDHTMKKNPVSIPHTVWYADDIRRGEREAADALGLTLYELMLR
                     AGEAAFQVCRSAYPDARHWLVLCGHGNNGGDGYVVARLAKAIGIDVTLLAQESDKPLP
                     EEAALAREAWLNAGGEIHASNIVWPESVDLIVDALLGTGLQQAPRESISQLIDHANTH
                     PAPIVAVDIPSGLLAETGATPGAVINADHTITFIALKPGLLTGKARDVTGQLHFDSLG
                     LDSWLAGQETKIQRFSAEQLSQWLIPRRPTSHKGDHGRLVIIGGDHGTAGAIRMTGEA
                     ALRAGAGLVRVLTRSENIAPLLTARPELMVHELTMDSLTESLEWADVVVIGPGLGQQE
                     WGKKALQKVENFRKPMLWDADALNLLAINPDKRHNRVITPHPGEAARLLGCSVAEIES
                     DRLHCAKRLVQRYGGVAVLKGAGTVVAAHPDALGIIDAGNAGMASGGMGDVLSGIIGA
                     LLGQKLSPYDAACAGCVAHGAAADVLAARFGTRGMLATDLFSTLQRIVNPEVTDKNHD
                     ESSNSAP"
     gene            16031..17170
                     /gene="queG"
                     /locus_tag="ACHKBL_002834"
     CDS             16031..17170
                     /gene="queG"
                     /locus_tag="ACHKBL_002834"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HFO5819684.1"
                     /translation="MSEPLDLNQLAQNIKQWGLELGFQQVGITDTDLSASEPKLQAWL
                     DKQYHGEMDWMARHGMLRARPHELLPGTLRVISVRMNYLPANAAFASTLKNPKLGYVS
                     RYALGRDYHKLLRNRLKKLGEMIQQHCVSLNFRPFVDSAPILERPLAEKAGLGWTGKH
                     SLILNREAGSFFFLGELLVDIPLPVDQPVEEGCGKCVACMTICPTGAIVEPYTVDARR
                     CISYLTIELEGAIPEELRPLMGNRIYGCDDCQLICPWNRYSQLTTEDDFSPRKPLHAP
                     ELIELFAWSEEKFLKVTEGSAIRRIGHLRWLRNIAVALGNAPWDETILTALESRKGEH
                     PLLDEHIAWAIAQQIERRNACVVEVQLPKKQRLVRVIEKGLPRDA"
     gene            complement(17153..17206)
                     /locus_tag="ACHKBL_002835"
     CDS             complement(17153..17206)
                     /locus_tag="ACHKBL_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5819685.1"
                     /translation="MRFYFYSQAVDESGVTR"
BASE COUNT         3965 a         4967 c         4365 g         4139 t
ORIGIN      
        1 tgttcaggta cagtttgcag cgccgtcgcc agcgccaacg gattccatgt catatattat
       61 tccatataaa ttaagtttaa atattaataa aatgaatatt tgcagtacgt aattatctta
      121 ccagctatag acaaaaaaaa accatccaaa tctggatggc ttttcataat tcagaggaac
      181 tagctgcgct gacgaaccgc ttcaaataag caaattccgg ttgcaaccga aacgttcagg
      241 gaagaaacac ttcctgccat tgggatgctg atcaactcat cgcaatgttc acgggtcagg
      301 cgacgcatac cttcaccttc cgcgcccatc accagcgcca ggcgtccggt cattttgctc
      361 tgatagagtg tatgatccgc ctcgcctgcc gtaccgacga tccagatatt ctcttcctgc
      421 aacatacgca tggtgcgcgc cagattagtc acccgaatca gcggaacgct ttctgccgca
      481 ccacaggcca cttttttcgc cgtggcgttg agctgtgcgg agcgatcttt cggcacaatc
      541 accgcatgaa caccagcggc gtccgcgcta cgcaggcacg cgccgaggtt gtgcggatcg
      601 gttacgccgt cgaggatcag caggaacggt tgatcgagcg aagcgatcag atccggcaga
      661 tcgttttcct gatactgccg tcctggcttc acacgagcga taatgccctg atgcacggca
      721 ccgtcgcttt tctcgtcgag atattggcgg tttgccaact ggataaccac gccctgagac
      781 tcaagggcgt gaatcagcgg taacagacgt ttatcttcac ggccttttaa aataaagact
      841 tcctgaaaac gttcaggggc gcgctccagc agggcctgca ctgcatggat gccgtaaatc
      901 atttcgctca ttaatgtact cgttatttac gttttgccgg gagcgccacg cttgcccggc
      961 ctacggataa ttcaccctct tctgtttgaa gagggtaatg atcactctgc cacttttttc
     1021 ttcgccgcac gcttcgcttt ggtcgctgcg gctattttct gcgttttcgc cgatggcttt
     1081 ttcgcttttc tcgcgtcttt cttcgctgct ttcggcttcg gttttttctc gccgcggaag
     1141 gcgctgtctg gctcaaagtt gacctttttg ccgacctgac gccgcttgcc gccttttttg
     1201 cctgcatcgc cttttttcgc tttctcgcgc gccgttttac cgacgttgcg cggtgcgcgt
     1261 tcgctggaga tcaggctaaa gtcgattttg cgctcgtcca tattaaccgc ttcgacgcga
     1321 acttccacgc gatcgcccag gcgataagtc tggccgctgg attcgcccat caggcgttgc
     1381 cccacctggt caaagcgata gtagtcattg tccagcgaag agacatggac cagaccatca
     1441 atgaacaagt catccaggcg aacaaagaag ccaaaaccag tgacgctgga aattacgcct
     1501 ttaaagacgt tacctacctg gtcgagcatg aagtcacact tcagccagtc agccacatcg
     1561 cgcgttgctt cgtcggcacg acgttccgcc atcgaacagt gctgacccag ttgcagcatc
     1621 tcttccatcg aataatggta gccgccggtt tcagtggtat tcccctgatg cccctgctct
     1681 ttcgccaaca gatacttaat ggcgcggtgc agcgtcaggt caggataacg acgaatcggc
     1741 gaagtaaagt gcgcatagga ctgcaatgcc agaccgaagt gaccacggtt ttccggatcg
     1801 taaatcgctt gtttcatcga gcgcagcagc atggtttgca gcatttctgc atcaggacgg
     1861 tcggcaaccg actccagcag ctctgcgtag tcgcgcggtt ccggcttgtt accgcccggc
     1921 agttccagcc ccagctccgc cagcactgaa cggaaagagg taatcgcttc ggtgctcggc
     1981 ttgtcgtgaa tacggaacag tgccggttct ttcgctttct caacgaaacg cgccgccgag
     2041 atattcgcca gaatcatgca ctcttcaatt aacttgtgtg cgtcgttacg ctgagtctgt
     2101 tcgatacgct caatacggcg ttcggcgttg aaaatgaact tcgcttcttc gctctcaaat
     2161 gagatcccgc cacgttcttc acgggcttta tccagcactt tatagaggtt atgcaactct
     2221 tcgagatgct taaccagcgg ggcatactgc tcgcgcagat cctgatcgcc ttgcagtata
     2281 tgccagactt tggtgtaggt cagacgcgcg tgagaactca tcaccgcttc gtagaatttg
     2341 tagccagtca ggcggccttt cgacgaaacc gtcatctcgc acaccataca caggcggtct
     2401 acctgcgggt tgagcgagca caggccgtta gagagcactt ccggcagcat cgggataacc
     2461 tgcgaaggga agtacaccga cgtaccacgg ttacgcgctt ctctgtccag cggtgttggc
     2521 ggacgcacat agtaactgac gtcggcaatt gcgacccata aacgccagcc gccgccgcgt
     2581 tttttctcac agtaaactgc atcgtcaaag tcacgggcgt cttcgccatc aatggtgacc
     2641 agcggtaaat cgcgcagatc gacacggccc gcttttgctt cttccggcac ttcttctttc
     2701 agcccggcaa cctgttgctc aacagcctgc ggccagatgt acggaatttc atgggtacgc
     2761 agagcaatat caaccgccat gccggtgccc atattatcgc ccagcacttc gacgatttta
     2821 cccaccgctt tggtacggcg agtcgggcgt tgagtcagtt caaccaccac cacaaagccc
     2881 atccgcgcgc ccatgatctg atcgggcggg attaagatat cgaagctcag acggctatcg
     2941 tcaggaacca caaagccgac gcccgcttcg gtgaagtagc gaccaacaat ctggctggtt
     3001 tttggcacca gtacgcggac aatacgcgct tcacgacgac ctttacggtc agcgcccagc
     3061 ggctgagcca gcacctgatc gccatgaatg caggttttca tctgctcgct ggagagatac
     3121 aaatcatctt tacgcccttc aactcgcaga aagccgtagc catcacggtg gccaataacg
     3181 gtacctttca ccaggtcgag gcgttccggc agcgcatagc actgacggcg agtgaagacc
     3241 agttgaccat cgcgctccat cgcgcgcagg cggcgacgca ggccttcaag ctgctcttcg
     3301 ccttcaatgt gcagttctac cgccagctca tcacggctgg ccggtttttc acgtttggtt
     3361 aaatgttcga ggataaattc ccgactaggg atgggattcg cgtatttttc agcttcgcgt
     3421 tcctggaaag gatcttgtga catctcggtt cctccgttgt catctctgat gaagattttc
     3481 gtcactccac cagcaataat ttataaagcg gttgattctc ttcaaccaaa tcggcaagcg
     3541 tgtagttatc cagttccgta agaaaacttt gcacggcctt agaaagcgcc tgtttcaacc
     3601 gacaggcagg tgtaatgtgg caaaactcac tgctgcaatt caccagcgat aagggctcca
     3661 gctcgcgcac cacatcacca atacgtattg cactcgccgg tttacccaag cgaatgccgc
     3721 cattttttcc acgaacagca gtcacgtagc cggcacgact aagttgattg attattttga
     3781 ccatatgatt acgggagacg ccgtagacgt cagtcacttc agaaatactg gtcatccgcc
     3841 cttctggcaa tgacgccatg tagatcagcg cacgtaatcc gtaatcagtg aaactcgtta
     3901 actgcacatc aacctcaaaa gggaaatcgg ggaaaaatac atttatattg atgataaacc
     3961 agccacaagc tgtgtcgcta atttatttca gttcggggaa ggaaaaaagc gaggatttaa
     4021 cactgtgtcg gatagcgtaa tggcttaacc gacacagcaa tgtatcacac tgttagcccg
     4081 gcaggcaaaa tatctgccag gcgtaccaga attacgcgtc gaacgggtcg cgcagaatca
     4141 tggtttcagt acgatccgga ccagtagaga tgatatcgat cggcacaccg gtcagctctt
     4201 caatgcgctt gatgtagttc agtgccgcct gcggcaggcc gctacgatct ttcacgccga
     4261 aggtggattc agaccagccc ggcatggttt cgtaaatcgg ctctacacct ttccagtcgt
     4321 cagctgccag cggagtggta gtcacttcac gaccatccgg catacggtaa gccacgcaga
     4381 gtttaacctc tttcaggcca tccagaacgt ccagtttagt caggcagaag ccagacagag
     4441 agttcagctg taccgcacga cgaacggcaa cggtgtccag ccagccggta cgacgacgac
     4501 gacccgtagt tgcgccgaat tcgttaccct gcttgcagag gaactcgcca gtttcatcaa
     4561 acagttcggt cgggaacgga cctgcaccca cacgagtgga gtaagctttg aggataccca
     4621 gaacgtaatc aacataacgc gggcccaggc cggaaccggt cgccacgcca ccagcagtgg
     4681 tgttggaaga agttacgtac ggatatgtac cgtggtcgat atccagcagc gtaccctgcg
     4741 caccttcaaa catgacgaaa tcgccacgct gacgcgcctg gtcaagcagg tcagaaacgt
     4801 caacaaccat agaagtcagg atgtcggcaa cagccatcgt atcatccaga actttctggt
     4861 aatcaaccgc ttcagctttg tagtagttaa ccaactggaa gttgtgatat tccatcactt
     4921 ctttcagttt ttcagcgaag gtttctttgt cgaaaaggtc gccaacacgc agaccgcgac
     4981 gtgccacttt atcttcataa gcaggcccga tacctcgacc ggtggtgccg atcgctttcg
     5041 cgccacgcgc tttctcacgc gcgttatcca gcgcaacgtg ataatcaagg atcagcggac
     5101 atgcttcgga cagcagcaga cgctcacgaa cggggatgcc acggtcttcc agttctttca
     5161 tctctttcat cagcgcagcc ggagacagca caacaccgtt accgatgatg ctggttacat
     5221 tctcgcggag aatacctgat ggaataagat ggagaacggt tttttcaccg ttgattacga
     5281 gagtatggcc tgcgttgtga ccgccctggt agcgtacaac atatttagcc cgttcagtca
     5341 gaagatcgac gatcttacct ttaccttcgt caccccattg ggtgcccagt acgacgacgt
     5401 tgttacccat ttttcaaaat caccgtttgc ttaaaaatgg attctaccat cgctttttca
     5461 gagttacagc actttttgca ctcaaaaatg acctcgatca gtctgttttt tgttcagcca
     5521 atcgttttcc tcaacatgta gtagaccaca acgcccgcaa ccacaagtcc accgccaaaa
     5581 cgacgtaaaa tattatcggg caaattggtc atcgcagaga tcatcttctt ccatgccttc
     5641 gggtaaagca tcggccctaa accttccagt accaaaacca gggcaagcgc cagccagatt
     5701 gtcgaattca ttcattatcc ttatagaaaa agaaaaccac cgacatccct gaggatgcgg
     5761 tggctttatt gacctgtacc gcagtcgtta tattaacgcg ttgcggaagt cggcgtcttc
     5821 atgtagcgga agaaatcgct atccgggctc atgaccatca cgtcctgatt gccagagaag
     5881 ctgttctcat aagcacgcag gctacggata aatgcgtaga agtccggatc tttactgaat
     5941 gcatcagcaa acagtttggc tgcttctgca tcgccttcac cacgcatgat gcggccctga
     6001 cgctcagctt cagccagcgt tctggtcact tcatagtcgg cagtcgcgcg cagtttttcc
     6061 gcttcttcct gaccttgtga acggtgacga cgcgctaccg cttcacgctc ggcgcgcata
     6121 cggttgtaga tcgcttcaga cacttcggtc ggcaggttga tctgcttaat acgcacatcg
     6181 acaacttcaa tacccagcgc cgccatactg ttcgggttga tgaccggaac tttgcccttc
     6241 gtctcagccg ttacgcgctc tgccgcttcg gcaatggcgt tatctgctgc cggggtagta
     6301 acttcatctt ctgtacccgc agaaccggag ttcagcgcgt cacgtacttc gagggtcaga
     6361 cgaccacggg aatcggtgac gatgtctttc acgtccaggc gaccaatttc agaacgcaga
     6421 cggtcagaga acttacgttt caacagcact tccgcttgcg aaatgtcgcc accacccgtt
     6481 gccaggtagt aacggctgaa atcgctgatg cgccatttga tgtaagagtc gacgatcagg
     6541 tctttcttct ctttggtcac aaagcggtcg gcctggttgt ccatggtctg aatacgtgcg
     6601 tcgagcattt tcaccgtttc aatgaacggt atcttgaaat gcagacccgg ctcataaacc
     6661 agaggtttgt tgtcatcgtc acgcagtacc ttaccaaaac gcagcgtaat accgcgctca
     6721 ccttctttga cgacaaagac agacatgtaa agcactacca gcacgatgat gataatcgcg
     6781 ataactgact tacgcatcgt tattccccct gacgctggta gtcgttacgc tgcgcgttgg
     6841 cgcggcgttg gtccataata tcgccctgac tggtggacga cgtgttgctt gctccactgg
     6901 ttgtggagga agaggctggc ggcagacgca gcagattgct ggcaccgtta tcgctcttcg
     6961 ccgcaggggc gttaccacct ttcagcatct ggtctaacgg cagaaccatc aagttgccac
     7021 ctttatcgtt aaccagcact ttgcgggtgt tgcccaacac tttttccatc gtctcgatat
     7081 acagacgctc gcgagtaatt tccggcgcgg ctttatattc cggcagaagt ttagcaaagc
     7141 gcgccacttc accctgagct tccaggatgg tctgggcctt gtacgcacgc gcctcttcga
     7201 ggatacgttg cgcctgaccg ttcgcacgcg gctgaacttc gttggtatac gcttctgctt
     7261 cacgaatgta ttgctgttcg ttttcacgcg cggcaatcgc atcgtcaaac gccgctttta
     7321 cttcttccgg cggacgagct gcctggaagt tgacgtccag cagcgtgata cccatgtcat
     7381 acggacgaat cgtctcttcc agttcgcgct gagtatcgct acgaatcacg gtacgacctt
     7441 ccgtcagaat gcggtccatg gtgtatttac cgataacccc acgcagggcg ctgtcggtag
     7501 cctgacgcag gctgtcatcc gggctggtca cgctatacag atatttttcc ggatcggtga
     7561 cgcggtactg cacgttcatc tcaacgcgca ctacgttctc gtccgacgtc agcatcacac
     7621 cagaagcggc cagttcacgc acggcttcca cgttcaccgg tttgacttcg tcgataaacg
     7681 tcggtttcca gttcagaccc ggctcaacca gatggctgaa tttaccaaag cgtgttacca
     7741 cgccacgttc ggcttcttta atggtataga aaccactggc cgcccagata atgacaatcg
     7801 ctgccgctgc gatggtaacg acacgaccgc caagctgcgg gcgcgggcct tgcgatgaac
     7861 tgccaccgcc agatccggtg cctttaccgc cgcccagacc accgagcttt ttgctcagtt
     7921 tgcggaagat atcatctaaa tcaggtggcc cttgatcgcg accgcctttg tttccatttc
     7981 cctcagagtt gccgccaggt ttgctgcttc cccacgggtc gcggtcttgt ccgttattac
     8041 cgggctgatt ccacgccatg tttgtgctcc atatttgtta tgcggtgatc cctgttgtct
     8101 ttcgacgcca gaggatatga ctccacgctt cagacgctac gccgttagat caggtaatcg
     8161 atcaacgccg gttcttgttt acagaggcga cgccagtcaa cgatcggcat acgaacttgc
     8221 agacttacgc tgccgtcctc ctccatccac tctttttcta ttgcctgaag ctgataaaaa
     8281 cgacttctca gacgcccttc ctgcggtggc agacgcaatg tatgctgcgc cacctcgccg
     8341 gaaagccgct ccgtcaaagc ctgaaaaagc tgtggtatcc cctctccggt ctgtgcggaa
     8401 agccagacac ggatcggttt gttctcttcg tcccgatcaa tacgcggttc gaaatcttcc
     8461 agcatatcga tcttgttcat caccagcagg gttgggatct cgtgagcgtc gatctcttca
     8521 agaaccgtat tcaccgcttc gatgttttct tgtacacgca catccgccgc atcaatgacg
     8581 tgcagcagca atgtggcttg ccgcgtctct tgtaacgtgg ctttaaatgc cgccaccaga
     8641 tcgtgcggca ggtggcgaat aaaccctacg gtatctgcca acacggtttc accgacatct
     8701 gcgacgtcaa tacgccgcaa cgtcgggtcg agggtggcaa acaactggtc tgccgcgtag
     8761 acccgcgctt cggtgatgcg attgaaaagg gtagatttac cggcgttggt atatcccacc
     8821 agcgaaacag taggaacgtc ggctttgata cgcgattgcc gcccctgctc acgctgcttt
     8881 tcaactcttt ccaggcgcga ctgtatctgc acgatgcgat tacgcaacaa acgacggtcg
     8941 gtttcgagct gggtttcacc cggaccacgc aaacctatcc cgcctttctg tctttcaagg
     9001 tgggtccagc cacgcaccag gcgcgtagcc agatggcgca gctgcgccag ctcaacctgc
     9061 aacttaccct catgggtacg cgcacgttgg gcgaaaatat ctaaaataag gccggtgcgg
     9121 tcgataacac gacactcgca caaacgctcc aggttacgct cttgcgccgg gctcagggca
     9181 tggtcaaaaa gaacgaccga agcacccgtc gctttgacag cttccgcaat ttcaactgct
     9241 ttaccttcac ctacaaaata ctttgggtgc ggcgctttac ggctaccggt aatcacctgc
     9301 aatgcttcga caccggcgga agagaccaga gattcaaact cctggaggtc ttccatatct
     9361 ttgtcttgcg taaaatagat gtgtaccagt accgcctgct caccagcatc ataacggtca
     9421 aacaagcgta taaccctcta aatagatcag cggggaacgc aggatcgctg gctccccgtg
     9481 taaaaaaaac agcccgaaac cttattcggt ttcttcgctg tcctgttgcg cggaagtatt
     9541 ctgcgcgctg ctaccatgat ggtagttact gctggtaccg ccaccggcgt tgttactgtg
     9601 atgagaaacc gggcgagacg ggacaacagt agaaatcgcg tgcttgtaaa ccatctggct
     9661 gaccgtgttt ttcaacagga tcacgaactg atcaaaagac tcgatttgcc cttgcagctt
     9721 aataccattc accaaataaa tagaaactgg aacacgttcc cgacgcagtg cgttcaggaa
     9781 cggatcttgt aaagattgcc ccttagccat tctctctttt ccttatatgc ttatttgtac
     9841 tttgaacctt tcgattctga aaaaattgcg cacgatacgt ctcaattgta cacattcagc
     9901 ctgcgatagc accaacaacc tgtaatactt cgtcacgcgc ttgttctggt ttttcactgt
     9961 caagccagtg aaccccttcc caaccacgca gccaggttat ctgccgcttc gccaactgtc
    10021 tcgtggcgca aacacctcga taaaccattt catcgtatga gatttcgcct tcaaggtaag
    10081 accacatctg gcgataaccc acgcaacgaa tggaaggcaa atccgtatgc aaatctcctc
    10141 gggcaaaaag cgcccggact tctgcttcaa aacctgaagc caacatctga tgaaaacgct
    10201 gctcaattcg ttgatggagc agttcacggc tcgccggggc gatggcgaac tgatgcacct
    10261 gatacggtag agcgtctcct gacgtttgcg tcagttccgt taaagtttta cccgaaatga
    10321 aaaaaacttc cagtgcccgg gaaagccttt gtggatcatt tggatgaatc cttgctgccg
    10381 caaccggatc tacctcctga agttgacgat gcaatgactc ccaaccttgc tctgccgcct
    10441 gttgctcaat tctggcccgc acttccgggt ctgccgacgg tagcggcgac aacccttcca
    10501 gcaatgcctt gaaatacaac atcgtaccgc ccactaacag tgggatccgc cccgccgcgg
    10561 tgatatcggc catttccgcc agcgcatcgc ggcgaaaatc agcagccgag taagcctgcg
    10621 acggatcgcg aatatccagc aatcggtgcg gcgcggcgag taactcttca gcgttcggct
    10681 tcgccgtccc gatatccatc cctttgtaaa taagggcaga atcaacgctt atcaactcta
    10741 ctggtaaaat tttacgcagc tcaatggcta acgccgtttt accggaggcc gtcggcccca
    10801 tcaaaaaaat cgccttaggc aggctcgcct tactgatatc actcatcttt cagggctttt
    10861 atcgccggat gtaaatcaac agattgtaac agaccacccg gcggcgtttt cacaagttgc
    10921 ggacataacc gttccacgtc cgccagcagg gttatggcct gcgccattga ccactgcgca
    10981 tgttcgctca tcagatttcg tgcaatccac tgcgcaatat tgccaggttc gaatacggac
    11041 tgcttcgcca ggtagcctat cagttcagga atcaagattt gtaaattttg ttggcgtaag
    11101 ggtaaaggca cggccctgat ggtcacatgc tgcgcatcgg actggaaatc aatacccaat
    11161 tccgccaggg cagactgcgc tttttctaat gcagattttt cttcgccaga aacttttagc
    11221 cgcagcggaa tcagcaacgg ctgggcgcaa acgggcgctt cacccggcgt caattgtgcc
    11281 tgacgcagcc aacgttctgc cactggcaag gctaaaagtg aaatgttgcc gtcgcgctcc
    11341 agcaacgcac agtcggaatg gacgatagtc agtacccgac caaagctttg actgttcgcc
    11401 gcaagtgcag gttcctgcgg ttctggcgct tttggttttt gcatcggcgc gggcgtttgc
    11461 aaaagctggc gatacacttc accttgctgt ttctggtaac ctggctgcgc attcggccag
    11521 ggggcagccg gacgactgcc tgaggcgggc gcaggagtgt aaagcggagc tactggctca
    11581 cgaactgccg gttcagcaaa atgattgcgc cccgccgcca cgcggttttc cgggatggcg
    11641 cgcggtgcag gttgtggttc atcgtccagc ggtagcggcg tttccagttg ctgttgtagc
    11701 acgctcagca cgccctgata gataaagtca tgcaccagac gcgactgatg gaaacgcact
    11761 tcgtgtttgg cggggtgtac gttgacgtcc acctgatgcg ggtcgatctc cagatacagc
    11821 acaaacgccg gttgttgatc ggcccccagt ttgtcttcgc aggcctggcg gatcgcatga
    11881 ttgatcaggc gatcgcgcat catgcgacca ttcacgtagc aatactgaat ttccgccagt
    11941 gcgggcgtgg tgtgattcgg atctgccacc cagccgcgca gcgtaagatc gccgtgttgc
    12001 cattcaatcg ccagcgcttg ttcgagaaaa gcggtgccgc aaatcgcccc taaacggcgc
    12061 tctttttgtc caccttccgg cacggcgcgg tactggcgga ccattttccc gttatgcgac
    12121 aggttgatcg tgacgtcgaa acgcgccagc gcaatgcggc ggatgatctc atcaatgtgg
    12181 ttaaattcgg ttttctcggt gcgcaggaat ttgcgccgcg ccggggtgtt gtagaacaga
    12241 tccagcacct ccagcgtagt ccccacagga tgcgccgccg gttttaccgt cacgtccata
    12301 tcgcgccctt cggcataggc ctgccaggct tcctgctgtt ctacggtgcg tgaagtgagc
    12361 gtcaggcggg aaaccgaact gatactcgcc agcgcctcac cgcgaaagcc caggctgata
    12421 atggcttcga gatcgtccag agaggcgatt ttactggtgg catgacgagc cagcgccagc
    12481 gccagctcat cttttttgat accgcagccg ttatcacgaa tgcggataag tttcgccccg
    12541 ccacgttcga tatcaatatc aatgcgcgtc gcacctgcat cgaggctgtt ttccaccagt
    12601 tctttgacta ccgacgcagg tcgctcgacc acctcacctg cggcaatctg gttcgccagt
    12661 tgtggcggta agacctgtat tggcatcaat cgtccttagt ttggcagcgt acgatctggc
    12721 gtcgtcaccg tactggcagt ttgtgccgtt gcaccctgcg gcgcagattg catcggatgc
    12781 gcaaggaaat aattgcgcag acctttataa atggcttctg ccagctgttg ttggtaatcg
    12841 tcgctcgcca gcaaacgttc ttcgctgttg ttgctgataa aaccggtttc gaccagtact
    12901 gatgggatat ccggcgaacg cagaacgcca aggctggcgt gttctggtcg acgtttatgt
    12961 atttcgccaa tgcgttgcaa ctggctgatc atactggtcg ctacatcata ccctacacgc
    13021 tgggaatgac cgaactgtaa atccagcacc gcctggctta aatagggatc agactgactg
    13081 ttcgccagca catcgcccgc tccgcccagc agctccgact gtttctcgtg ctgctccagc
    13141 cagctggcca tttcgctgtt ggcgcgacgg ttagagagca cccatacgga agcgccagtc
    13201 gcactgcggt ttggtgcggc atcagcgtga atcgacacga ggaaattggc gttttgctta
    13261 cgtgccacat cgctgcgccc catcaccgag ataaagtaat ccccgtcacg ggttaaaacg
    13321 cctttaaaca ttggatcgtc attgagaaaa gtacgcaatt tacgtgcgat ggcgatggtg
    13381 acatttttct cccgcgtacc accggggccg atagcgccag ggtcctgacc gccgtgtcct
    13441 gcatcaatag cgatgataat tttatcgcca gtgttagccg tcgcgcgcgc tgccggacgc
    13501 gttaccgtat tactgctgat aacacctgta gtgcggttac tttccgtttt aaacggattg
    13561 cgcgccggtt cgctgacgcg cggtgcgaca accgcaggcg tttcaacgcg tttagcaacc
    13621 acaggcggcg aaggaggcgg tggcggcaca tcggcgttaa tcgtaaagac gacagtgtaa
    13681 ttgctgccat tctgccgctt caccgcttcg gttttaccgt tttcggttag atcgaccacc
    13741 agccgtagcg tttgtgcatc tttaggcgtt ccggagcgaa tcgccttcac cagattattg
    13801 ccgctaaaca acaacggcag tccctgaatc acgcccgttt gtttgatatc gagcgccacg
    13861 gtgcgtttgc tttgatggct aaacgcataa tcaggatcgc caataaaact caacgttatc
    13921 cgcgcctgct gattgccgtt agaaacctga atatcagaga gcgtcgcggc acccaccggc
    13981 gcgcacagca gcagcaacgt cgctaccaac caatttctga tgcgatacat catcccgcca
    14041 cctttcaaag gttaaccggc taaacgcgcc agcaacaatt cacccgcaga ggaaaccgca
    14101 ctcacgcgcg cctcacggcc ttgtgcctga taatcaatgt gtatttcgac atccgggtca
    14161 ggaagaacac ctgtaccttg ttgtggccac tccaccaggc agatggcatc gttggcaaaa
    14221 taatcgcgga tccccataaa ctccagctcc tcgggatcgg caaggcggta caaatcaaag
    14281 tgataaacca ttaaattgtc gagcgtatag ggttcgacca gcgtataagt ggggcttttg
    14341 acattaccct gatggcccaa agcctgcaaa aagccccggc taaaggtggt tttacctgcg
    14401 cctaaatcgc catacagata gattacggtt gcgccatcgc aggctttcgc tacccgctcg
    14461 cccaggtcta atgttgcctg ctcatcaggg agcggaatta ctcgattcat catggttttt
    14521 atcagtcact tccgggttaa caatacgctg tagcgtggaa aagagatcgg ttgccagcat
    14581 cccgcgcgtt ccaaaacgcg ccgccagtac gtcagctgcc gcaccgtgcg cgacacagcc
    14641 cgcacaggct gcatcatacg gcgacagttt ttgcccaagc aatgcgccaa taataccaga
    14701 gagcacatcg cccatgccgc cgctcgccat gcctgcattc ccggcatcaa taatgcctaa
    14761 agcgtcagga tgggcggcga ccacggttcc ggcacctttc agcaccgcta cgccgccata
    14821 acgttgtacc agacgtttgg cgcaatgtaa gcggtcactt tcaatttcag cgacggaaca
    14881 gcctaacaac cgtgcggcct cgccaggatg tggcgtgatc acgcgattgt gacgcttatc
    14941 gggattgatt gccagcaggt tcaatgcatc ggcatcccac aacatcggtt tgcgaaaatt
    15001 ctcaactttt tgcagggctt ttttccccca ctcttgctgg cccagaccgg gaccaatcac
    15061 caccacatcg gcccattcca ggctttcggt aagagagtcc atagtcagtt catgcaccat
    15121 caattctggt cgtgcagtca gcagcggcgc aatgttctca ctgcgggtca gtactcggac
    15181 taaaccagca ccagcacgta gcgccgcttc ccccgtcata cgaatagccc ccgccgtgcc
    15241 gtgatcgcca ccgataatca ccagccgccc gtgatcgcct ttatgcgaag tcgggcgacg
    15301 cggtatcagc cactgagaaa gttgttctgc cgaaaaccgc tgaattttcg tctcctgacc
    15361 tgccagccag ctatccagcc ccagtgagtc aaaatgcagt tgtccggtaa catcccgcgc
    15421 ttttccagtg agcaagcctg gtttcagcgc aataaaagtg atggtgtgat cggcgttgat
    15481 taccgcgcct ggcgtagcgc cggtttcagc cagaaggccg gaagggatat caaccgccac
    15541 aatcggcgca gggtgagtat tagcgtggtc gattaactgg ctaatggatt cgcggggcgc
    15601 ttgctgcaag ccggtgccga gcagcgcatc aacaatcaga tctaccgatt cgggccagac
    15661 aatattcgaa gcatggatct cgcctcccgc gtttaaccat gcttcgcgtg ccagcgcggc
    15721 ctcttccggc aacggtttgt cactttcctg ggccagcagc gtgacatcaa taccgatcgc
    15781 tttggccagc cgcgcgacca cgtagccatc gccgccgtta ttaccatgac cgcacagcac
    15841 cagccagtgg cgggcgtcag gataagccga acgacacacc tggaatgcgg cttcgccagc
    15901 gcgaagcatc agctcataga gtgtgagccc cagcgcatct gccgcctcgc gttctccgcg
    15961 gcggatatcg tcggcgtacc agacggtgtg tggtatactt acggggtttt tcttcattgt
    16021 atggtccgtc atgtcagagc ccctcgatct caatcaatta gcgcaaaata ttaaacagtg
    16081 ggggctggaa ctgggctttc agcaggtagg tattaccgat accgatctca gcgcgtccga
    16141 gcccaaactg caagcatggc tggacaaaca ataccacggc gaaatggact ggatggcgcg
    16201 tcacggtatg ctgcgtgctc gtcctcacga attattgccc ggtacgctgc gcgtgatcag
    16261 cgttcggatg aattaccttc ctgctaacgc cgcatttgcc agcacgctga aaaatcccaa
    16321 actcggctat gttagccgtt atgcgctggg ccgtgactat cacaagcttc tgcgcaaccg
    16381 actcaaaaag ctgggcgaga tgattcagca acattgtgtt tcgctgaatt ttagaccgtt
    16441 tgtcgattct gcgcctattc tcgagcgccc gttagctgaa aaagcaggtc ttggctggac
    16501 aggtaagcac tcacttatcc tcaatcgcga ggccggttcg ttcttctttt taggcgaatt
    16561 gctggtcgat attccgctgc ccgtggatca accagtcgag gaaggatgcg gtaaatgcgt
    16621 ggcctgtatg acgatttgcc cgaccggtgc catcgtcgag ccatataccg tcgatgctcg
    16681 ccgctgtatc tcttatctca ccatcgaact ggaaggggcg atcccggaag agttgcgacc
    16741 gttaatggga aaccgtattt acggttgcga tgactgccag cttatctgcc cgtggaatcg
    16801 ctattcgcaa ctcactacag aagacgattt cagcccgcgt aagccgctac acgcaccgga
    16861 actcattgag ttattcgcct ggagtgaaga gaagttttta aaagtcacgg aaggttcggc
    16921 gattcgccgt ataggtcacc tgcgttggct gcgtaatatc gccgtagcat taggcaatgc
    16981 cccctgggat gaaacgattt tgacagcgct ggaaagtcgt aaaggtgagc acccacttct
    17041 tgatgagcac atagcgtggg cgattgcgca gcaaatcgag aggcgaaatg cgtgcgtggt
    17101 cgaagtgcaa ttacccaaaa aacagcgtct ggttcgggtg attgaaaaag ggttaccgcg
    17161 tgacgcctga ttcatccaca gcttgtgaat aaaaataaaa acgtattgca attcaagacg
    17221 aataaaatcg tcaagtgatc gactcgacaa aacgaaatct taatttttaa cttatctatc
    17281 aataggttaa aaaaatacta ttcaccgtgc gaagtttctt tgaaattaag attacggcct
    17341 gaggctgtgg ataactctgt ttacaagagt ttgtcagaga caacaaaaac cagcaataac
    17401 gcatcgcgtc gctgtggata ttttattgag agaaga
//
LOCUS       DAZFLD010000103        17319 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.104, whole genome shotgun sequence.
ACCESSION   DAZFLD010000103 DAZFLD010000000
VERSION     DAZFLD010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17319
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.104"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..515
                     /locus_tag="ACHKBL_002836"
     CDS             <1..515
                     /locus_tag="ACHKBL_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HFO5819686.1"
                     /translation="KFKKCLLPVAMLASFTLAGCQSNADDHAADVYQTDQLNTKQETK
                     TVNIISILPAKVAVDNAQNKRNAQAFGALIGAVAGGVIGHNVGSGSNSGTTAGAVGGG
                     AVGAAAGSMVNDKTLVEGVSLTYKEGTKVYTSTQVGKECQFTTGLAVVITTTYNETRI
                     QPNTKCPEKS"
     gene            531..1070
                     /locus_tag="ACHKBL_002837"
     CDS             531..1070
                     /locus_tag="ACHKBL_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5384 family protein"
                     /protein_id="HFO5819687.1"
                     /translation="MKKVFLCAILASLSYPAIASSLQDQLSAVAEAEQQGKNEEQRQH
                     DEWVAERNREIQQEKQRRANAQAAANKRAATAAANKKARQDKLDAEATADKKRDQSYE
                     DELRSLEIQKQKLALAKEEARVKRENEFIDQELKHKAAQTDVVQSEADANRNMTEGGR
                     DLMKSVGKAEENKSDSWFN"
     gene            complement(1163..2740)
                     /gene="guaA"
                     /locus_tag="ACHKBL_002838"
     CDS             complement(1163..2740)
                     /gene="guaA"
                     /locus_tag="ACHKBL_002838"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFO5819688.1"
                     /translation="MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEA
                     QIRDFNPSGIILSGGPESTTEENSPRAPQYVFEAGVPVFGVCYGMQTMAMQLGGHVEA
                     SNEREFGYAQVEVVNDSALVRGIEDALTADGKPLLDVWMSHGDKVTAIPSDFVTVAST
                     ESCPFAIMANEEKRFYGVQFHPEVTHTRQGMRMLERFVRDICQCEALWTPAKIIDDAV
                     ARIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVLDM
                     FGDHFGLNIVHVPAEDRFLSALAGENDPEAKRKIIGRVFVEVFDEEALKLEDVKWLAQ
                     GTIYPDVIESAASATGKAHVIKSHHNVGGLPKEMKMGLVEPLKELFKDEVRKIGLELG
                     LPYDMLYRHPFPGPGLGVRVLGEVKKEYCDLLRRADAIFIEELRKADLYDKVSQAFTV
                     FLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWAHLPYDFLGRVSNRIINEVNGIS
                     RVVYDISGKPPATIEWE"
     gene            complement(2809..4275)
                     /gene="guaB"
                     /locus_tag="ACHKBL_002839"
     CDS             complement(2809..4275)
                     /gene="guaB"
                     /locus_tag="ACHKBL_002839"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HFO5819689.1"
                     /translation="MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLP
                     TTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLNQPVSVYMTPKERLV
                     TVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRL
                     RVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGG
                     NVATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIP
                     VIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTI
                     DELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS"
     gene            4437..5813
                     /gene="xseA"
                     /locus_tag="ACHKBL_002840"
     CDS             4437..5813
                     /gene="xseA"
                     /locus_tag="ACHKBL_002840"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000937953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HFO5819690.1"
                     /translation="MLPSQSPAIFTVSRLNQTVRLLLEHEMGQVWISGEISNFTQPAS
                     GHWYFTLKDDNAQVRCAMFRNSNRRVTFRPQHGQQVLVRANITLYEPRGDYQIIVESM
                     QPAGEGLLQQKYEQLKAKLQAEGLFDQQLKKPLPSPAHCVGVITSKTGAALHDILHVL
                     KRRDPSLPVIIYPTAVQGDDAPGQIVRAIELANLRNECDVLIVGRGGGSLEDLWSFND
                     ERVARAIFASRIPIVSAVGHETDVTIADFVADLRAPTPSAAAEVVSRNQQELLRQVQS
                     THQRLEMAMDYYLANRTRRFTQIHHRLQQQHPQLRLARQQTMLERLQKRMSFALENQL
                     KRAGQQQQRLTQRLNQQNPQPRIHRAQTRIQQLEYRLAETLRAQLSATRERFGNAVTH
                     LEAVSPLSTLARGYSVTSAADGAVLKQVKQVKVGETLTTRLGDGVVISEVSAVTKTRK
                     SRKKTSNP"
     gene            complement(5882..13933)
                     /locus_tag="ACHKBL_002841"
     CDS             complement(5882..13933)
                     /locus_tag="ACHKBL_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001535498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF823 domain-containing adhesin"
                     /protein_id="HFO5819691.1"
                     /translation="MSLTLNKILVLCALLISAMLPGWSWAESAWQDSSDTVGEFNGTV
                     PTADSASIPVYQGSVFLDPAKTHEVAFTAKPSEFSADVSVSKLLVTNPQDREGDIIAT
                     PRWENQTPPTVSLVWADAATPDTLLDPQPVADRSFCAQGLAGRSLIAWAQPDPQQTMP
                     LLYLLTSTGYPYESVLTLADQKVTLKIAPAQGDLISVSAAGYDESSGAAKTTVGGSIT
                     LTVTTKDCVGNVVGNIPFVIKRKDAENRQGVVNNTAPVKLGTTELTTTATEYRGTTDA
                     NGVATVTVTQANGPGVKTPLVASLAGIAQTSETAVIFTVLTSPDVPQATMWGHMPDTL
                     KARDYTFSRPKLAAEVDNEDGTVNDHNETWSTFTWSGADKHCDILPGMRQFGALATVV
                     PTSVQDVAGWPMQGNYYWSSLAGMSGQHYAADVSNRSEAQKPDDTTFIVSCVDKEAPD
                     VEPKLVLTPGSYDSTIKAMKVKVGEEASLRLTITDSKNNDQPLAYYYFSLHLDDGVNR
                     KNQTDAAWETHPVQIDGGSNVRKVDAHTYEGITDANGEASLTLTQPGGVGVKTHITAK
                     MRSDYTASDEKDVIFTVITSPDSDKARMWGHMLGIIEANNIFKRPRLADETDNELGAV
                     RENNEDWALFDQNSSMQAECGLGHIPSQSSLHSLFAAHPANVIGTEYGWPTLQKAYLS
                     AVEETSHASVNLATGNIDTYSGFKQNYLSCSGNEMVAQIAATTDHDVSTSSRAQAKVG
                     ETITMTVRTFNALNNAPVPYTAFTITKDMGKNRQGQTTGFDDPTRGAIEMNGTLYGTS
                     QPSLVYAGTTDAQGFATVEIKQPQGVGLATPLNIAPVNSYIPNTVNYNVIFTTLTSPD
                     AVGAQMWGHMDETITVDALTFARPRLAAEVSSPDGTLTENNEVWSRVSQANTSSTSKG
                     GCGANMLPRRSQLNALYDANSGNAVQTVHGWPTQRQPYWSSSPADQVPHYYTIALNDG
                     ARTVGGSTAVYVSCLTTANNPASSITLEVVDPAQWNAAANAAKLKKGETLQVKVTVKD
                     AQGNPLGDMPFTLKRGDGYTRSNEKHIAGGGDALVAPVVINSGLADETSLNDTAAAYS
                     AMTGSDGTKILTITRPDTHGTKTSLTAALYSDTTKKATLDTIFTVVTSPDSDKAKMWG
                     HMPETVTAADGTVFKRPLLLKELSSTSGRTAIAEENEDWAQFTQTQAISTSSNGCGSE
                     YVPSQAGLESLYEANRGNAMKTVQGWPVASSYLSSTTGSSSLEQRDFKAVNLSSGTSS
                     IIPSATKELLTCQTTPIVKASQIVLEAADPTKFDSTNNVVKAKKGEEAVLRVTTKDAQ
                     GNPVGNTAFTLKRNTSVNRANVSTTTSIASLAVTDAWGNTQDDFLSTTLVIYGVTGAD
                     GITTFTLKQDQTTGLKTELTAALDSSSSTKSTLPVVFTVLTSPDSPKAKFWGHMAETA
                     TGDDGLIYRRPLLRDENSATTSIGTLVEEGEAWSTFPSGQANDTSINGCGAEYVPTDN
                     ELRAIYAHQGSSALHDAIGWPVSRFYISNTVADTFTQTFTYDVVSLKTGDETQMPSSG
                     GALLSCRTTPVAVASQIIVEANDTAQFVKVDDTLSALKVKKGEDAVIRVVTKNAQGNS
                     VPNVPFILRREGSKNRQNAEMINKSITVINAAGASARMNSSSSLLYGVTGADGTTSFT
                     VKQDDSMGLVTNMYAQLYQPTIESNKLPVMFTVITSPDTPLASYWGHMPETFTTRSGI
                     AFKRPLLTAEHPAGQSTMANNESWLSLNTAAKNDVSKSDCGEPYQPLLSEFQELYSEH
                     PNGAIGTDLGLPLTNTWWAYDKIAYANVWYDQSINLSNGSSSRALSNTVAFVSCLVNP
                     HAVAASIEMTSTALDAEKTASNDGRPSATAAKGTAIRMTVIVRDSAGNLLPGANFNLI
                     RGTALDRAKNRLDSTYDDLTIVPVTPAGVNMSLYNNGAQALLTTGSDGKATFDVTQNE
                     TYGLATPLTATLMRDTTKSATMDVIFTVITSPNSPKAKYWGHMPDTFTSRAGVTFKRP
                     LLAAEATLGSSVSNNNESWSYLFYTNKVTPDCPVEYQPRLNELQGLYNDHPGGTILTD
                     LGLPITAGSGNWWTYEMSTTDALTWYYGVINLKTGQSTTTINGYALMLCLTQPHSAPA
                     SLTLSSTAYDEGRTASNGGTPTSSVKKGEMLPIVVTIKDANGNPVGGEGVTLKRVQAK
                     SRSGISVSSNTVDDLILDEVTPTSARISFNQNTSAWSGFTGSDGTITFNVTQNNTVGL
                     VTPFTASLARNPQVTANQDLIFTVVTSPDSAKANYWGHMPATLTAVNGAVFERPKLWS
                     ELTSTSGVGKINNNNEDWPYFTPTQKSDASVSPCEVARQPLFNDLSSLSARYPNNTFV
                     TETGWPAYYTWWAEDKSADGKDQSVDLRNGTLYTGSTKSFQPCLANARSTVSSVTLTS
                     TAFDAATQAAKVKKGEAMPVTVTVKDSAGNTVPNVEFTLKRGEASPRNAGATLYGDVV
                     AMDDLIVQPLSGSAVTLSESGNTISGMTGADGTASFTLRQDNTPGYKTPLTVTLANYA
                     SATDTLDAIFTVPTSPNVSSAHFWGHMADTVVVNGKSLHRPLLTTELPSGANPVSSPI
                     INYENWASAHIIDASKWDIARQCGSIENAPTYNELELLHTVFNSLGWPSSPSFPYLSS
                     QQCGMDEGTGAQDCSITLINKPGLVTCFQ"
     gene            complement(14054..15031)
                     /locus_tag="ACHKBL_002842"
     CDS             complement(14054..15031)
                     /locus_tag="ACHKBL_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SinI family autotransporter-associated protein"
                     /protein_id="HFO5819692.1"
                     /translation="MKQDKRRGLTRIALALALAGYCVAPVALAEESAWVDSGETNIFQ
                     GTIPWLYSEGGSATTDADHVTLTSDLKGARPTGSETDKRLYSGDKLTVSWEIGDTEGD
                     VDLGGLGDNAKTIDTIRWMSYKDAQGGDPKELATKVTSYTLTDDDRGRYIGIEITPTT
                     QTGTPNVGTALHLYDISTASGGGSDSDNVAPGPVVNQNLKVAIFVDGTSINLINGSTP
                     IELGKTYVAKLYSDENKNGKFDAGTDADVTANYDFRWVLSGSSQQLGTSGGIVNSSFD
                     NNNLAIPATNDEARTNLNGPARDGKEALTIPTNGDGVQGYKLHIIYKHK"
     gene            complement(15086..17263)
                     /gene="sinH"
                     /locus_tag="ACHKBL_002843"
     CDS             complement(15086..17263)
                     /gene="sinH"
                     /locus_tag="ACHKBL_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001317981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intimin-like inverse autotransporter SinH"
                     /protein_id="HFO5819693.1"
                     /translation="MLRWKRCIILTFISGAAFAAPEINVKQNESLPDLGSQAAQQDEQ
                     TNKGKSLKERGADYIINSATQGFENLTPEALESQARSYLQSQITSTAQSYIEDTLSPY
                     GKVRSNLSIGQGGDLDGSSIDYFVPWYDNQTTVYFSQFSAQRKEDRTIGNIGLGVRYN
                     FDEWLLGGNIFYDYDFTRGHRRLGLGGEAWTDYLKFSGNYYHPLSDWKDSEDFDFYEE
                     RPARGWDIRAEAWLPSYPQLGGKVVFEQYYGDEVALFGTDNLEKDPYAVTLGLNYQPV
                     PLLTVGADYKAGTGDNSDVSINATLNYQFGVPLKDQLDSDKVKAAHSLMGSRHDFVER
                     NNFIVLEYKEKDPLDVTLWLKADATNEHPECVIKDTPEAAVGLEKCKWTINALINHHY
                     KIVAASWQAKNNAARTLVMPVIKENTLTEGNNNHWNLVLPAWQYSSDKAEQEKLNTWR
                     VRLALEDEKGNRQNSGVVEITVQQDRKIELIVNNIANVPEENNHSHEASAQADGVDGV
                     VMDLDVTDSFGDNTDRNGDALPEDNLTPRLYDAQDKRVTLTNKPCSTDNPCVFIAKQD
                     KEKGTVTLSSTLPGTYRWKAKAAPYDDSNYVDVTFLGAEIGGLNAFIYRVGAAKPSNL
                     IGKDKEPLPLNNTYRFVLWRDSNKDGVFQQVEKLTDEEMAQYDYKWEFTGKSINGEVG
                     AQANTSNEDIVIPATNREAAQTYGAQAGDGLQGYGLRVLYTKK"
BASE COUNT         4068 a         4611 c         4301 g         4339 t
ORIGIN      
        1 tgaaatttaa aaaatgtctt ctgcctgtgg caatgttagc gtcattcact ctggcaggat
       61 gccagtcaaa tgctgatgat catgccgccg atgtttatca aaccgatcaa ctgaatacca
      121 aacaagaaac taaaaccgtt aatattattt ccattcttcc cgcaaaagtt gccgtagaca
      181 acgcccaaaa taaacggaac gcacaagcct tcggcgcgct tattggcgct gtcgctggcg
      241 gtgttatcgg ccacaacgtc gggtctggca gcaattccgg aacgacggca ggtgcagttg
      301 gcggcggagc tgtaggcgcg gcagcgggtt ctatggtgaa tgataaaacc ttagtggaag
      361 gtgtttcttt aacatataag gaaggcacca aagtgtatac ctccacccag gtgggtaaag
      421 agtgccagtt tacgacaggt ttagccgttg ttattaccac gacgtataac gaaacacgta
      481 ttcagccaaa taccaaatgt cctgaaaaga gctaataatc aggaggagtc atgaagaaag
      541 tttttctttg cgccatctta gcctccttaa gctatccggc tatcgcctca tcattgcagg
      601 atcaactctc ggctgtcgca gaagcggaac agcaaggtaa aaatgaagag caaaggcagc
      661 atgacgaatg ggtcgcggag cgcaacaggg aaatccagca agagaagcaa cgtcgcgcaa
      721 acgcccaggc cgcggctaat aaaagagcgg caacggcagc ggcgaataag aaagctcgtc
      781 aggataaact ggacgccgaa gccactgcgg acaaaaaacg cgatcaaagt tatgaagatg
      841 agctacgcag cttagagatt cagaaacaaa aactggcgct ggcgaaagaa gaagcccgcg
      901 ttaagcgaga aaacgaattt atcgatcagg aactgaagca caaagctgcg caaaccgatg
      961 tggtgcaatc tgaagctgac gcaaacagga atatgactga aggcggtcgc gatctgatga
     1021 aaagcgtggg caaagcagaa gagaataaat cagatagctg gtttaactaa acgatgttag
     1081 taacttcaaa cctataattc tttaggataa aaaaccctct gtagtaacag agggttttgt
     1141 tcattcatag tgcagggtca aatcattccc actcaatagt tgctggtggc ttgccgctga
     1201 tgtcatacac cacgcgggaa ataccattca cttcattgat aatgcggttg gaaacgcgac
     1261 cgaggaaatc gtacggcaga tgtgcccagt gtgcggtcat aaagtcgatg gtttcgacag
     1321 cacgcagaga gacaacccag tcatacttac gaccatcgcc cattacgcca acggaacgta
     1381 ctggcaggaa gacggtgaat gcctggctga ctttgtcgta caggtccgct ttacgcagtt
     1441 cttcaatgaa gatggcgtca gcacggcgca gcaggtcaca atactctttc ttcacttctc
     1501 ccagaacacg aacgccaagg cctggtcccg ggaacgggtg acggtacaac atgtcatacg
     1561 gtaggcccag ctccagacca atcttacgca cttcgtcttt gaacagctct ttcagcggtt
     1621 caaccaagcc catcttcatc tctttcggca ggccacccac gttgtggtga gatttgatga
     1681 cgtgtgcttt accggttgca gaagcagcag attcgataac gtcagggtag atagtgccct
     1741 gcgccagcca cttcacgtct tccagtttca gcgcttcttc atcgaatact tcaacgaaaa
     1801 cgcgcccgat gattttacgt tttgcttccg gatcattttc gccagccagc gctgacaaga
     1861 agcgatcttc tgccggtacg tgaacaatat tcagaccaaa gtgatcgcca aacatatcca
     1921 gaacctgctc tgcttcgttg agacgcagca ggccgttgtc gacgaatacg caagtcaggt
     1981 ttttaccgat agcgcggtgc aacagcattg cggttacgga ggaatccaca ccaccagaga
     2041 ggccgaggat gactttatcg tcgcctacct gctcgcggat gcgagctaca gcatcgtcga
     2101 taattttcgc tggcgtccac agggcttcac actggcagat atcacgcaca aaacgctcca
     2161 gcatgcgcat accctggcgg gtgtgagtca cttccgggtg gaactgtacg ccatagaagc
     2221 gtttttcttc gttagccata atggcaaacg ggcagctttc ggtgctggct acggtgacga
     2281 agtcggacgg gatagcggta actttatcgc cgtggctcat ccagacatcg agcagcggtt
     2341 taccgtctgc ggtcagagca tcttcaatac cgcgaaccag tgcgctgtcg tttacgactt
     2401 caacctgcgc gtagccaaat tcacgttcgt tagaggcttc aacgtgaccg cccaactgca
     2461 ttgccatggt ctgcatgcca tagcaaacgc cgaataccgg tacgcctgct tcaaaaacat
     2521 actgcggcgc acgcgggctg ttttcttcgg tggtgctttc cgggccgccg gaaagaataa
     2581 tgccgcttgg gttgaagtca cgaatttgtg cttctgtcac atcccacgcc cacagttcgc
     2641 agtaaacacc cagttcacgc acgcggcgcg caaccagttg agtgtactgg gaaccgaagt
     2701 ccagaatgag gatgcgatgc ttatgaatgt tttccgtcat tgacgcttat tccgaggcaa
     2761 gtgaaacaga taatataaat cgcccgacat gaagtcgggc gaagagaatc aggagcccag
     2821 acggtagttc ggggactctt tagtaatggt cacgtcgtga acgtggcttt cctgaatgcc
     2881 cgcaccgctg atacgtacaa actccgcttt agtacgcagt tcgtcgatag taccacagcc
     2941 ggtcagcccc atacaggagc gcaggccgcc catctgctgg tgaatgatct ctttcaggcg
     3001 acctttatag gccacgcgac cttcgatacc ttccggcacc agtttgtcgg cagcgttgtc
     3061 gctctggaag taacggtcag aagaaccttt ggacatcgcg cccagggaac ccataccacg
     3121 gtaagatttg taagaacggc cctggtagag ttcgatttca cccggagatt cttcagtacc
     3181 tgccagcatg gaacccacca tcaccgcgct tgcgcctgcg gcgatagctt tggcgatatc
     3241 gcctgagaaa cggataccgc cgtcagcgat aaccggaatg ccagtacctt ccagggcttc
     3301 tactgcatca gcaacggcgg tgatctgcgg aacaccgaca ccagtcacga tacgcgtcgt
     3361 acagatagaa ccagggccga tacccacttt aacggcactg caaccggctt ctgccagcgc
     3421 gcgggcacct gcagctgttg ccacgttgcc gccgatgatt tgcaggtcag gatatttagc
     3481 gcgagtttca cggatacgct gcagaacacc ttcagagtga ccgtgagagg agtcgatcag
     3541 cagaacgtca acgcctgcgg caaccagcgc atcaacacgc tcttcgttac cagcgccagc
     3601 accaaccgct gcaccaacac gcagacggcc ttgctcgtct ttacaggcgt tcggtttacg
     3661 ttctgctttc tggaaatctt tcacggtgat catgccgatc aggtggaatt cgtcatcaac
     3721 caccagcgct ttttcaacgc gtttttcgtg catttttgcc agcaccactt cacgggcttc
     3781 gccttcacgc acggttacca gacgctcttt tggcgtcatg taaacgctaa ccggttgatt
     3841 cagatcggtc acaaaacgca cgtcacgacc ggtgatgatg ccgaccagtt cgttttcttc
     3901 ggtaacgacc gggtagcccg caaagccgtt acgctcggtc agttctttca cttcgcgcag
     3961 cgtcgtggtt ggcagaacag tctgcgggtc agtcaccaca ccagattcgt gttttttcac
     4021 acggcgaact tcttctgcct ggcgttcaat ggacatgttt ttgtggataa agccgatacc
     4081 gccttcctga gccagagcaa tagccaggcg cgcttccgtt acggtatcca ttgctgcgga
     4141 aagcataggg atattcagac gaatagtttt cgtcagctgg gtgctgaggt cagcagtatt
     4201 cggcagaacg gtagaatgtg caggaacgag gagaacgtcg tcaaacgtca gagcttcttt
     4261 agcgatacgt agcatgggca atatctcgac cagagtggtt aataaatatt gccgcggcat
     4321 tatacagagc gtaaccgatt gcatctaccc ctttttgcaa aaaatgcttg ctatccccgg
     4381 agggcgggtt actatcgact gaataacctg ctgatttaga atttgatctc gctcacatgt
     4441 taccttctca atcccctgca atttttaccg ttagccgcct gaatcaaacg gttcgtctgc
     4501 tgcttgagca tgagatgggg caggtgtgga tcagcggcga aatctctaat ttcacacaac
     4561 cggcttccgg tcactggtac tttacgctta aagacgataa cgcccaggtg cgctgcgcga
     4621 tgttccgcaa cagcaatcgc cgggtcacct tccgcccgca gcatgggcaa caggttttag
     4681 ttcgcgccaa cattacgctg tatgaaccgc gcggcgatta tcaaattatt gtcgaaagta
     4741 tgcagcccgc cggtgaaggt ttactgcaac agaagtacga acagcttaaa gcgaagttgc
     4801 aggctgaagg tttgttcgat cagcaactta aaaaaccact cccctcccct gcgcactgcg
     4861 ttggggtgat tacctcaaaa acaggtgccg cactgcacga tattttgcat gtgttgaaac
     4921 gccgcgatcc gtctctgcca gtgataatct atcccactgc cgttcagggc gatgacgcac
     4981 cagggcaaat cgttcgcgcc attgagctgg cgaatcttcg caacgagtgc gacgtattaa
     5041 tcgtcgggcg tggcggtggc tcgctggaag atttatggag ttttaatgat gagcgtgtgg
     5101 cgcgggcgat ttttgccagt cgcattccga tagtcagcgc cgtcgggcat gaaaccgatg
     5161 tgacaattgc tgactttgtt gctgatctac gtgcgccaac gccgtcagcc gccgctgaag
     5221 tggtgagccg aaatcagcaa gaattgctac gtcaggtgca atcgacccat caacggctgg
     5281 aaatggcgat ggattattat ctcgccaacc gcacgcgtcg ttttacgcag attcatcatc
     5341 gcttgcagca acagcatccg cagctccggc tggcacgtca gcaaactatg ctcgaacgtc
     5401 tgcaaaagcg gatgagcttt gcgctggaaa atcagcttaa gcgtgccggg caacagcagc
     5461 agcgattaac acagaggcta aaccagcaaa atccgcagcc gcgtattcac cgcgcacaga
     5521 cgcgaattca acaactggaa tatcgtttag cagaaaccct gcgcgcacag cttagcgcca
     5581 cgcgtgaacg tttcggtaat gcagtaacac acctcgaagc cgtaagccca ctgtcaacgc
     5641 tggcgcgcgg ttatagcgtc accagcgccg ctgatggcgc ggtgttaaaa caggttaagc
     5701 aagtgaaagt gggtgagaca ctgaccacac gactgggaga tggtgtggtg attagtgaag
     5761 tcagcgccgt aacaaaaacc cgcaaatcac gtaaaaaaac gtctaatccg tagaccggat
     5821 aagaggcgcc agcatcgcat ccggcaataa ctcccaggcc acgccagatc cgccatgctg
     5881 gttactggaa gcaggtcacc agacccggtt tatttattag cgtgatacta caatcctgtg
     5941 ctccggtacc ttcgtccatc ccacattgtt gagaggacaa ataaggaaaa ctgggtgaag
     6001 acggccatcc cagtgaatta aaaacagtat gcaacaactc taattcattg tatgtcggtg
     6061 cattttctat gctgccacac tgccttgcga tatcccattt actggcatca atgatatgtg
     6121 cagaagccca gttttcgtag ttaataatcg gtgaggatac gggattagcc ccggacggca
     6181 attctgtcgt taacaacggt cgatgtagcg atttaccatt caccactacc gtgtctgcca
     6241 tatgtcccca aaagtgcgcc gaggaaacat ttgggctggt tggtaccgta aaaatagcgt
     6301 ccagcgtgtc tgtcgcggaa gcgtaattag ccagagtaac cgtcagtggc gttttataac
     6361 caggggtatt atcctggcgg agcgtaaaac ttgccgtccc atccgcgcct gtcatgccac
     6421 taatggtatt accgctttca ctcagcgtaa cagccgaacc agataacggc tgaacgatga
     6481 gatcgtccat cgccaccaca tcaccataca acgttgcccc agcgttacgc ggcgatgcct
     6541 caccgcgttt caaagtaaat tccacattcg gcaccgtatt cccggcgcta tctttcaccg
     6601 tcacggtaac gggcatggct tcgccttttt tcactttcgc cgcctgagtg gctgcatcaa
     6661 acgctgttga ggtaagtgtg acgctggaaa ccgttgaacg cgcgttagcc aggcaaggct
     6721 gaaatgattt tgttgaaccc gtatacagcg tgccatttct caaatcaacc gactggtctt
     6781 taccatcggc ggatttatcc tctgcccacc aagtgtaata cgcaggccag ccagtttcag
     6841 tgacaaacgt gttattcgga taacgcgcag acaacgaact taaatcattg aatagtggct
     6901 gccttgctac ttcacaaggg ctcacactcg catctgattt ctgtgttggg gtaaagtacg
     6961 gccagtcttc attattgtta ttgatcttgc cgacgccaga agtcgatgtc agttcactcc
     7021 acaatttcgg gcgttcaaat accgcaccat tcactgccgt caaggttgct ggcatatgcc
     7081 cccagtagtt agccttcgca gaatccgggc tggtcactac cgtgaatatc aggtcctgat
     7141 tagccgtaac ctgcggattt cgcgccaatg aagctgtaaa cggcgttacc agaccgacgg
     7201 tgttattttg ggtaacgttg aacgttatcg tgccatcact gccggtgaag ccgctccatg
     7261 ccgacgtatt ctggttgaag gagatacgcg ccgatgtcgg ggtgacttca tctaaaatga
     7321 gatcatctac ggtattggag gatacagaga tccctgaacg gcttttcgcc tgcacgcgct
     7381 tgagcgtaac accttcgccg ccaacaggat taccattggc atctttgatc gtcaccacga
     7441 tgggaagcat ctcacctttt ttcacagatg acgtgggggt accaccattg ctggcagttc
     7501 ggccctcatc ataggcagtc gatgacagcg taaggcttgc tggtgccgaa tgtggctgcg
     7561 tcagacagag cattaaagcg tatccattaa tagtcgtcgt cgattgtccc gttttcaaat
     7621 taatgacgcc gtaataccac gtcaacgcgt cagtggtgct catttcataa gtccaccaat
     7681 ttccacttcc ggcagttata ggcaacccca aatctgtcag tattgtcccg cctggatgat
     7741 cattatatag cccctgtaac tcattgagac gcggctgata ctcaaccggg caatccggcg
     7801 taactttatt ggtataaaaa aggtatgacc acgactcatt attattactt accgaggacc
     7861 ccaaggtcgc ttctgctgcc agtagcggtc gtttaaaggt cacccccgcc ctactggtaa
     7921 acgtgtcggg catatgaccc cagtattttg ctttcggtga gttcggactg gttatcacag
     7981 tgaaaataac atccatcgtg gcactttttg tggtgtcgcg catcaatgtt gcagtgagcg
     8041 gagttgccag cccataagtc tcgttttgcg tcacatcaaa agtcgcttta ccatcacttc
     8101 ctgtcgtcag aagtgcctgt gccccattat tatagagtga catgtttact ccggcagggg
     8161 tgaccggcac gatagtaaga tcatcatacg tagaatctag cctgtttttt gcacgatcca
     8221 acgcagtccc gcgtatgaga ttaaagtttg cgcctggcaa cagatttcct gcgctatccc
     8281 gcacaatcac cgtcatccgg atcgccgtac ctttcgccgc cgttgcacta gggcgaccat
     8341 cgttgctggc cgttttctcc gcatccagtg ccgtggaggt catctctata gaagctgcca
     8401 ctgcatgagg gtttaccaga caggacacaa atgccacggt gtttgataat gccctggagc
     8461 ttgatccatt cgataaatta atagactgat cgtaccagac gttcgcgtag gcaattttat
     8521 cgtaagccca ccaggtgtta gtcagcggaa ggcctaaatc ggtccctatc gcaccattag
     8581 gatgttcact gtataactct tgaaattcgc ttaataacgg ttgataaggt tcgccgcagt
     8641 ctgatttgct gacatcattt tttgctgccg tattcaaaga aagccatgat tcattattag
     8701 ccatggttga ctgccccgct ggatgctcgg cagtaagcaa tggccgctta aaggcaattc
     8761 ccgaccgggt agtaaacgtt tcaggcatat gcccccagta acttgccagt ggcgtatccg
     8821 ggctggttat gacggtaaac atgaccggta atttgttgga ttctatcgtc ggctgataca
     8881 actgggcgta catattggtt accaggccca tagaatcatc ttgttttacc gtaaaggagg
     8941 tagtcccatc cgcgccagtc accccataaa gcaggcttga agaactattc atcctcgcag
     9001 aagcgcctgc cgcgttaatt accgtaatcg acttatttat catctcggca ttttgacggt
     9061 tcttcgagcc ttcacgtctg agaataaagg ggacattggg cacgctattt ccctgcgcat
     9121 ttttagtcac cacgcgaatc accgcgtctt cgcctttttt cactttcaac gcactcaagg
     9181 tgtcgtcaac tttgacaaat tgcgccgtat cattcgcctc aacgatgatc tgactggcaa
     9241 cggcgacagg cgtggtccgg cagctcagta atgcaccacc agatgagggc atctgcgttt
     9301 catctcctgt cttcagcgaa acgacatcat acgtaaaggt ttgagtgaac gtatctgcaa
     9361 cggtattcga gatatagaac ctcgacactg gccagccaat agcatcatgt aatgcgctac
     9421 tcccctggtg cgcataaatg gccctaagtt cattgtcagt tggaacatac tccgcgccac
     9481 aaccatttat cgaggtatcg ttagcctggc ccgacgggaa cgtagaccag gcctccccct
     9541 cttccaccag ggtaccgata ctggtggtag cactattctc gtccctcagt aaaggccttc
     9601 gataaataag accgtcatcg cctgtcgccg tttcagccat atgcccccag aatttggctt
     9661 ttggcgaatc cgggctggta agcaccgtaa agacaacggg aagcgtcgat ttagtgctgc
     9721 tggaggaatc caacgctgcc gtgagttctg tttttagccc ggtagtctgg tcttgcttca
     9781 atgtgaaagt ggtgatgcca tcagcccccg tcacgccata aataaccagg gtcgttgaaa
     9841 gaaaatcatc ttgagtattt ccccaggcat ccgttaccgc cagcgaagca atcgacgtcg
     9901 tagttgacac attggccctg ttcacgctgg tgtttcgttt gagggtaaat gcggtatttc
     9961 ccacagggtt accctgcgca tctttagtgg tcactcgtaa tacggcttct tcgccttttt
    10021 tcgctttgac aacattattc gtactgtcaa attttgtggg gtctgcagct tcaagaacaa
    10081 tttggcttgc cttaacaatc ggcgtcgtct ggcaggtaag cagttctttt gtggcagatg
    10141 gaattatcga actggtgcct gaactgagat tgacggcctt gaagtcacgc tgttccaggc
    10201 tactactacc tgtcgtgctg gaaaggtaac ttgaggcgac gggccagccc tgaaccgttt
    10261 tcatcgcatt gccgcggttg gcttcataca atgactccag cccggcctgg gagggaacat
    10321 attcactacc acaaccatta gaggatgtgc ttatagcctg ggtttgggta aattgcgccc
    10381 agtcttcatt ctcttctgca atcgctgttc gacccgacgt actggataac tctttcaaca
    10441 ataatgggcg tttaaagacc gttccatctg cagccgtcac tgtttcaggc atatgccccc
    10501 acattttcgc cttatcgctg tccgggctgg tgacaacggt aaaaatggta tcgagggtcg
    10561 cttttttcgt tgtgtctgaa tagagcgcgg cggtcagcga cgtttttgtg ccatgggtat
    10621 ccgggcgggt aatagtgaga attttagtgc catcgctgcc ggtcatcgcg ctgtatgctg
    10681 cggcggtatc attgagcgaa gtttcatccg ccaggccact attgatcaca accggcgcaa
    10741 ccagcgcatc accgccaccg gcaatgtgtt tttcgtttga tcgggtataa ccatcgccac
    10801 gcttcagcgt aaaaggcata tcacccaatg gattgccctg agcatctttt accgtcacct
    10861 tcacctgtag cgtttcgcct tttttcagct tcgccgcatt tgctgccgcg ttccactgcg
    10921 ccgggtccac cacttccagc gtaatgctgc tggcaggatt gttagccgtc gtcaggcaac
    10981 tgacataaac cgccgtgcta ccgccaacgg tccgcgcgcc atcgttgagg gctatggtgt
    11041 agtaatgcgg cacctgatcc gcgggcgaac tgctccagta gggctgacgc tgcgttggcc
    11101 aaccgtgtac cgtttgaacg gcgttaccgc tgttagcatc atagagcgcg ttaagctggg
    11161 aacggcgtgg gagcatattg gcaccacagc cacctttact ggtgctggaa gtgttggcct
    11221 ggctaacgcg cgaccacact tcgttatttt ccgttaatgt gccgtccggg ctggacactt
    11281 ccgcagccag ccggggtcgg gcaaaggtta atgcatcgac ggtaatcgtc tcatccatat
    11341 gaccccacat ctgcgcgcca accgcatccg gactggtgag cgtagtgaaa atcacattgt
    11401 aattaacagt attgggaata taggagttta caggcgcaat attcagcggc gtcgctaagc
    11461 ccacaccttg cggttgctta atttccacag tagcaaaacc ctgcgcatcc gtagtaccgg
    11521 catagactaa agacggttgc gatgttccgt agagtgtgcc attcatctcg attgcgccgc
    11581 gggttggatc atcaaaacct gtagtctgcc cctgacgatt tttccccata tctttagtga
    11641 tggtgaacgc ggtatacggc acgggcgcat tattcaaggc attaaaggtg cgtaccgtca
    11701 tggtaatcgt ttcacccact ttagcctgcg ctctgctgct tgtggaaaca tcatgatcgg
    11761 tggtcgcagc aatctgggcg accatctcat tgcccgaaca agaaaggtag ttttgcttaa
    11821 agccggaata ggtgtcgatg ttgcccgtgg ccaggttcac cgacgcgtgc gacgtctctt
    11881 ccaccgcact aagatacgct ttttgcaacg ttggccagcc atattcggtg ccgatgacat
    11941 tggctggatg tgcggcaaac aaactgtgca atgagctttg gctcggaata tgacctaaac
    12001 cacactcagc ctgcatactg ctattctgat caaacagcgc ccagtcttcg ttattttccc
    12061 gcactgctcc cagttcgttg tcggtttcat ccgccagccg tgggcgttta aagatattat
    12121 tcgcttcaat aatgcctaac atatgtcccc acatccgcgc tttgtcggaa tcagggctgg
    12181 tgatcaccgt aaaaataacg tctttctcat ccgaggcggt ataatcactg cgcattttcg
    12241 cggtgatgtg tgttttcacc cccacgccgc ctggctgggt cagcgtcaga gaggcctctc
    12301 cgttagcatc agtgatccct tcataggtat gcgcgtcaac tttgcgaacg ttactgccac
    12361 catcaatttg taccgggtgc gtctcccacg cagcgtcagt ttgattctta cggttaacac
    12421 cgtcatccag atgcagcgaa aagtagtagt aagccagcgg ctgatcgttg ttcttgctat
    12481 cggtaatcgt caggcgtaaa gaagcctctt cccccacctt tactttcatc gcttttatcg
    12541 tcgaatcata actgcccggc gtcagtacca gcttcggttc aacatcgggc gcttctttat
    12601 cgacacagct cacaataaac gtcgtatcgt ccggtttttg cgcttcgctg cggttcgaaa
    12661 catccgccgc atagtgctgc ccgctcatcc ccgccagtga tgaccagtag tagttaccct
    12721 gcatcggcca accagccaca tcctgcactg acgttggcac caccgtcgcc agcgcaccga
    12781 actgacgcat tccgggcaaa atgtcgcaat gtttatccgc accactccag gtaaaggtgg
    12841 accaggtttc attgtgatcg ttaaccgtac cgtcttcatt atcgacttcc gccgccagtt
    12901 ttggtcgact aaaagtgtaa tcacgggcct tcagcgtatc gggcatatga ccccacattg
    12961 tggcctgcgg gacatccggg ctggtgagga cggtaaagat cactgccgtt tcactggtct
    13021 gagcaatacc cgcgaggctc gcgaccaacg gcgtttttac accagggccg ttggcctggg
    13081 tgaccgtaac cgtggcaaca ccattcgcat cggtagtacc acgatattcc gtcgcggtgg
    13141 tggttaattc ggtcgtgccc agttttaccg gcgcagtatt gttcactaca ccctggcggt
    13201 tttcagcatc tttgcgtttg ataacaaaag gaatattccc cacaacatta ccgacgcaat
    13261 ctttggtggt gaccgttaac gtgatactgc cgccaaccgt cgttttcgcc gcaccggagg
    13321 attcgtcata gcccgcggcg ctgacggaaa taaggtcacc ctgcgcgggc gcaatcttca
    13381 gcgtcacttt ctgatcggca agggtcagaa cactttcata gggatagccc gtcgaggtaa
    13441 gtagatataa cagcggcatg gtctgctggg ggtctggctg cgcccaggcg atcagcgaac
    13501 gcccggcaag gccctgtgcg caaaatgagc gatctgcgac tggctgtggg tccagtaagg
    13561 tgtccggcgt cgcggcatcc gcccaaacta aagatactgt cggtggcgtt tggttttccc
    13621 aacgtggtgt ggcaatgata tcgccttctc tgtcttgcgg gttggtgacg agcaatttag
    13681 agacgctaac atccgcgctg aattcactcg gtttagcggt aaacgctacc tcatgcgtct
    13741 ttgcaggatc cagaaagaca ctgccctggt agacgggtat gctggcactg tcggcggtgg
    13801 gcacggtacc gttaaattcg ccgaccgtat ctgaagagtc ttgccatgca gattctgccc
    13861 atgaccaccc aggcagcatt gcgcttatca gcaatgcgca taacacaagg attttattca
    13921 gggtcaggga catgacagaa actccaccgg ttgttaaacg gacgcttgtc gcatcaacat
    13981 attttgctta atccgggatt aagcaaaata tgcggaaaca gcattccctt gcgggaatgc
    14041 tgctgagtac gcgttatttg tgtttgtaaa taatgtgaag tttgtaaccc tgtaccccgt
    14101 cgccgtttgt cggaatggta agagcctctt taccatcgcg cgcaggaccg ttaaggttgg
    14161 ttctggcttc gtcgttggtc gcagggatgg ccagattgtt attatcgaag cttgagttaa
    14221 cgataccacc tgaagtgccc agctgctggc tgctgccaga aagtacccaa cggaagtcat
    14281 aattggcggt aacgtcagca tcggtacccg catcaaactt gccgtttttg ttctcatccg
    14341 agtacagttt ggccacgtag gttttgccaa gttcgattgg cgtgctgccg ttgataaggt
    14401 tgatactggt accatcaaca aagatggcga ctttcaggtt ctggttaacc accggccctg
    14461 gcgcaacgtt atcgctgtcg cttccgccgc cgctggcagt ggaaatgtca taaagatgca
    14521 gcgcagtccc gacgtttggc gtaccggtct gcgtggtcgg cgtaatttca ataccgatat
    14581 aacgaccacg gtcgtcatcg gtaagagtgt aactggtcac cttcgttgcc agctctttcg
    14641 gatcgccccc ctgagcgtcc ttgtagctca tccagcggat agtatcgata gtctttgcgt
    14701 tatcgcccaa tcccccaaga tccacgtcgc cttcggtatc accaatttcc caacttacgg
    14761 ttaatttatc acccgagtaa agacgcttgt ccgtttcact gcctgtcggg cgagcgcctt
    14821 ttagatcaga agtcaacgtt acatggtcgg catctgtcgt agcgcttccc ccttccgaat
    14881 agagccacgg aatggttccc tggaaaatat tggtttcgcc gctgtcgacc caggcgcttt
    14941 cttccgccag cgccacaggt gccacacaat aacctgccag tgccagcgcc aatgcgatcc
    15001 gggtcagacc gcgtctttta tcctgcttca ttgatgctct ccttaatgaa ccttcagcga
    15061 ctggccgact ggccttacgc caatcttatt ttttggtata cagcacgcgt aaaccgtatc
    15121 cctgcaagcc atctcccgct tgtgcgccat aggtttgcgc tgcttcgcgg ttagtggccg
    15181 gaatcacaat gtcctcattg gaagtgttcg cctgtgcacc cacttcacca ttgatgctct
    15241 tgccggtaaa ttcccacttg tagtcgtact gcgccatctc ttcgtcagtc agtttttcga
    15301 cctgctggaa gacaccgtct ttattgctgt cacgccacaa aacaaatcga taagtgttgt
    15361 tgagcggcag cggttcttta tctttaccta tcaggttgct gggtttagcc gcccccacac
    15421 gatagataaa tgcatttagc ccaccaattt ctgccccgag gaaagtgaca tccacatagt
    15481 tactgtcgtc gtagggcgca gcttttgctt tccagcgata ggtgccgggt aaggtactgg
    15541 agagggtgac agtacccttt tctttatctt gtttagcgat aaaaacgcag gggttgtcgg
    15601 tcgagcaagg cttgttggtt aacgtcactc gtttgtcctg cgcgtcgtaa agccgcggcg
    15661 tgaggttatc ttccggcagc gcatcgccgt tgcggtcggt gttatcgcca aagctgtcgg
    15721 ttacatcgag atccatcacc acaccatcaa cgccatctgc ctgggcgctg gcttcgtggc
    15781 tgtggttgtt ctcttccggc acgttcgcga tgttattaac aatcaactca attttacggt
    15841 cctgctgaac ggtgatttcc accacaccag agttctgtcg gttgcccttt tcatcttcca
    15901 gcgccagacg tactcgccag gtattgagtt tttcttgttc ggctttgtca gagctgtact
    15961 gccaggcggg cagcaccagg ttccagtggt tattgttacc ctctgtcagg gtattctctt
    16021 tgataaccgg cataaccagc gtgcgggcag cattgttttt cgcctgccat gacgcagcga
    16081 caattttgta gtggtggttg ataagcgcgt taatggtcca cttacatttt tccagcccga
    16141 ccgccgcttc aggcgtgtct ttaatgacac actcggggtg ttcgttggtg gcatccgctt
    16201 ttaaccacag ggtgacatca agcggatctt tttctttgta ttccagaaca ataaagttat
    16261 tacgctcaac aaaatcatga cgactgccca tcaacgagtg agccgctttc actttatcgc
    16321 tatccaattg atcttttagc ggaacgccga actgataatt aagagtggca ttaatgctga
    16381 catcgctgtt atctccggtc cccgctttat agtccgcccc aacggttagt aatggcaccg
    16441 gttgataatt cagtccaagt gttaccgcgt agggatcttt ctccaaatta tccgtaccaa
    16501 aaagggcaac ttcatcgccg taatattgct cgaagacaac tttgcccccc agttgcggat
    16561 aagaaggtaa ccaggcttcg gcgcgaatat cccagccgcg cgcaggacgc tcctcataaa
    16621 aatcgaaatc ttcggagtct ttccagtcag aaagcggatg ataatagttg ccagagaatt
    16681 ttaaataatc agtccacgct tcgccgccta aacctaaacg gcgatgtcct cgggtaaaat
    16741 cataatcata aaatatattt ccgcccaata accattcatc aaaattatat cttaccccaa
    16801 gaccaatatt cccgattgta cgatcttctt ttcgctgcgc agaaaattga ctgaaataaa
    16861 cagtggtttg attatcgtac cagggaacaa aataatcgat ggaactgcca tccagatcgc
    16921 cgccctggcc aatggagagg ttcgaacgga ccttaccgta gggagagaga gtgtcttcaa
    16981 tataagattg tgcggttgag gtgatttgac tttgcagata gcttctggcc tgagattcca
    17041 gcgcctcagg ggtcaagttt tcaaaccctt gcgtggcgga gttgatgatg taatcggctc
    17101 cgcgctcttt cagcgattta cccttgttgg tttgttcatc ctgctgtgct gcctggctac
    17161 ctaaatcagg taacgattcg ttttgcttaa catttatctc tggcgccgcg aaagcagcac
    17221 cagagataaa tgttagaata atacagcgtt tccatcgcag catgcacttt ccctgtgaca
    17281 atgatacagt caaaaataag ctttaatatt gtatccctg
//
LOCUS       DAZFLD010000104        17281 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.105, whole genome shotgun sequence.
ACCESSION   DAZFLD010000104 DAZFLD010000000
VERSION     DAZFLD010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17281
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.105"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..122)
                     /locus_tag="ACHKBL_002844"
     CDS             complement(<1..122)
                     /locus_tag="ACHKBL_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011192680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HFO5819694.1"
                     /translation="MLKNLLNTEVVQVVEQVKDWREAVAISCRPLIENGSIEPR"
     gene            393..1424
                     /locus_tag="ACHKBL_002845"
     CDS             393..1424
                     /locus_tag="ACHKBL_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFO5819695.1"
                     /translation="MEKTRKRRGTGRVTLQEVANFAGVGTMTVSRALRTPEQVSDKLR
                     EKIEQAVEELGYIPNRTAGALASGHSHTVAVLVPSLTDKASSRFMQSLQQVLNKNEFQ
                     LLLGCHEYNQLKEAEILMTLLQGNPAALVIFGSQLADKTHQILEKTNIPTINVIGSPF
                     SPAQITIETAFFEASHKLTEHLLEQGYKNIGFIGAHMDNRLQRQQLNGWHKAMLSHYQ
                     NTDLVITMPDAASLQLGRYALNEILLRQPELDAVICSHEEIALGIMFECQRRLLKIPG
                     NIAVACLDGSDNCDQTHPTLTSIRIDYKKMGIETGKLLIGLLNNNHDESEESRIVQFN
                     YQIELRQST"
     gene            1821..2090
                     /locus_tag="ACHKBL_002846"
                     /pseudo
     CDS             1821..2090
                     /locus_tag="ACHKBL_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001167450.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2264..3493)
                     /locus_tag="ACHKBL_002847"
     CDS             complement(2264..3493)
                     /locus_tag="ACHKBL_002847"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase"
                     /protein_id="HFO5819696.1"
                     /translation="MTRRVVITGMGGVTAFGENWQDVSARLLAYENAVRKMPEWQVYD
                     GLHTLLGAPVDDFTLPEHYTRKRIRAMGRVSQMSTRASELALEQAGLIGDPILTSGET
                     GIAYGSSTGSTGPVSEFATMLTEKHTNNITGTTYVQMMPHTTAVNTGLFFGLRGRVIP
                     TSSACTSGSQAIGYAWEAIRHGYQTVMVAGGAEELCPSEAAVFDTLFATSQHNDAPKT
                     TPSPFDENRDGLVIGEGAGTLILEELEHAKARSATIYGEIVGFATNCDAAHITQPQRE
                     TMQYCMEQSLKIAGLSAQDIGYISAHGTATDRGDMAESLATATIYGDNVPLSSLKSYF
                     GHTLGACGALEAWMSLQMMREGWFAPTLNLNKPDPNCGALDYIMHEARKVDCEFLQSN
                     NFAFGGINTSIIIKRWP"
     gene            complement(3490..4221)
                     /locus_tag="ACHKBL_002848"
     CDS             complement(3490..4221)
                     /locus_tag="ACHKBL_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-ketoacyl-ACP reductase FabG2"
                     /protein_id="HFO5819697.1"
                     /translation="MSRSVLVTGASKGIGRAIACKLAADGFNIGVHYHRDATGAQETL
                     NAIVANGGNGRLLSFDVANREQCREVLEHEIAQHGAWYGVVSNAGIARDAAFPALSDD
                     DWDAVIHTNLDSFYNVIQPCIMPMIGARQGGRIITLSSVSGVMGNRGQVNYSAAKAGI
                     IGATKALAIELAKRKITVNCIAPGLIDTGMIEMEESALKEAMSMIPMKRMGQAEEVAG
                     LASYLMSDIAGYVTRQVISINGGML"
     gene            complement(4221..4685)
                     /locus_tag="ACHKBL_002849"
     CDS             complement(4221..4685)
                     /locus_tag="ACHKBL_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-fatty acyl-ACP dehydratase"
                     /protein_id="HFO5819698.1"
                     /translation="MSHYLSPGAYLPHDAPMLLLEDVVSVSDDSAVCRVTVSPSGVLA
                     PFLDPDGNLPGWFALELMAQTVGVWSGWHRHQQGKNSIELGMILGARELLCTTGILPA
                     GQTLTITVKLLMQDDRFGSFECSINDGEATGRINTFQPTAEELTTLFQQGAS"
     gene            complement(4682..5851)
                     /locus_tag="ACHKBL_002850"
     CDS             complement(4682..5851)
                     /locus_tag="ACHKBL_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HFO5819699.1"
                     /translation="MIYISAVGMINALGNNLDEIAANLARGVAPGMRPRAGWLQGHPQ
                     AVLAGVDGELPLIPEKFAAHRSRNNQILLAALAQIQPQVDDAIAKYGRERIAIVLGTS
                     TSGLHEGDTHVNLRTNGQPSTTWHYAQQELGDPSRFLSHWLALDGPAYTLSTACSSSA
                     RAMISGRRLIEAGLVDAAIVGGADTLSRMPINGFHSLESLSPTLCQPFGRDRAGITIG
                     EGAGLMLLTREPQPIALLGVGESSDAYHISAPHPQGEGAIRAINQALTDAQLTPDDIG
                     YINLHGTATQLNDQIESMVVNALFGKRIPCSSTKHLTGHTLGAAGITEAAISMLILQR
                     DLPLPAQDFSLSPRDPTLPPCGIIEKPQPLARPVILSNSFAFGGNNASILLGRVS"
     gene            complement(5853..6437)
                     /locus_tag="ACHKBL_002851"
     CDS             complement(5853..6437)
                     /locus_tag="ACHKBL_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3261 domain-containing protein"
                     /protein_id="HFO5819700.1"
                     /translation="MIKSTFWRALALTATLILTGCSHSQPEQEGRPQAWLQPGTRITL
                     PAPGISPAVNSQQLLSGSFNGKTQSLLVMLNADDQKITLAGLSSVGIRLFLVTYDAKG
                     LRAEQSIVVPQLPPASQVLADVMLSHWPISAWQPQLPTGWTLRDNGNKRELRNASGKL
                     VTEIIYLNRQGKRVPISIEQHVFKYHITIQYLGD"
     gene            complement(6434..8752)
                     /locus_tag="ACHKBL_002852"
     CDS             complement(6434..8752)
                     /locus_tag="ACHKBL_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HFO5819701.1"
                     /translation="MTNANVLPPSKRPALLWGLVCLVMAVVLLILLPQSRLNSSVLAM
                     LPKQAMGDIPPALNDGFMQRLDRQLVWLVSPGKEANPRVAQEWLTLLQKSAALGDIKG
                     PMDAASQQAWGAFFWQHRNGLIDPDTRARLQNGGEAQAQWILSQLYSAFSGVSGKELQ
                     NDPLMLMRGSQLAMAKNGQRLRLMDGWLVTQDPQGNYWYLLHGELAGSSFDMQQTHQL
                     ITTLNTLEKDLKTRYPQAQLLSRGTVFYSDYASQQAKQDISTLGVATLLGVILMIVAV
                     FRSLRPLLLCVISIGIGALAGTAATLLIFGELHLMTLVMSMSVIGISADYTLYYLTER
                     MVHGNDVSPWQSLAKVRNALLLALLTTVAAYLIMMLAPFPGIRQMAIFAAVGLSASCL
                     TVLFWHPWLCRGLPVRPVPAMALMLRWLAAWRRNKKLSLGLPVALALFSLAGMSMLRV
                     DDDISQLQALPQHILAQEKAITALTGQSVDQKWFVVYGDSPQQTLGRLETFTTSLEKA
                     KKDGLISNYRTIPLNSLARQEEDLDLLKTAAPTVTKALQNAGLTAVNPDLNAMPVKVD
                     EWLASPASEGWRLLWLTLENGESGVLVPVEGVKSSALQQEIASYYPGGIAWVDRKSTF
                     DELFALYRYVLTGLLLVALAVIACGAVARLGWRKGLISLVPSVLSLGCGLAVLAMSGQ
                     AVNLFSLLALVLVLGIGINYTLFFSNPRGTPLTSLLAITLAMLTTLLTLGMLVFSATQ
                     AISSFGIVLVSGIFTAFLLSPLAMPDKKRTKK"
     gene            complement(8721..9326)
                     /locus_tag="ACHKBL_002853"
     CDS             complement(8721..9326)
                     /locus_tag="ACHKBL_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HFO5819702.1"
                     /translation="MKFLPLLALLISPFVSALTLDDLQQRFTEQPVIRAHFDQTRTIK
                     DLPQPLRSQGQMLIARDQGLLWDQTSPFPMQLLLDDKRMVQVINGQPPQIITAENNPQ
                     MFQFNHLLRALFQADRKVLEQNFRVEFADKGEGRWTLRLTPTTTPLDKIFNTIDLAGQ
                     TYLESIQLNDKQGDRTDIALTQHQLTPAQLSDDERQRFAAQ"
     gene            complement(9323..9745)
                     /locus_tag="ACHKBL_002854"
     CDS             complement(9323..9745)
                     /locus_tag="ACHKBL_002854"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HFO5819703.1"
                     /translation="MLNDPRFTAEVELTIPFHDVDMMGVAWHGNYFRYFEVAREALLN
                     QFNYGYRQMKESGYLWPVVDARVKYRHALTFEQRIRVRAHIEEFENRLRIGYQIFDAE
                     TGKRATTGYTIQVAVEEQSRELCFVSPDILFERMGVKP"
     gene            complement(9749..11425)
                     /locus_tag="ACHKBL_002855"
     CDS             complement(9749..11425)
                     /locus_tag="ACHKBL_002855"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFO5819704.1"
                     /translation="MSVNFSPCVLIPCYNHGAMMPGVLARLKPFNLPCIVVDDGSDAA
                     TQQQLDNLVAEQPGVTLIRLAENTGKGAAVMRGLQAAADAGFSHAVQVDADGQHAIED
                     IPKLLALAEQHPAALISGQPIYDDSIPRSRLYGRWVTHVWVWIETLSLQLKDSMCGFR
                     VYPVAPTLQLAKYATIGKRMDFDTEVMVRLYWQGNTCYFVPTRVTYPQDGLSHFDALK
                     DNVRISLMHTRLFFGMLPRIPSLLMRRSSSHWARQSEVKGLWGMRLMLLVWRLLGRTA
                     FSALLYPVVGVYWLTAARARKASQDWLARVRQHQPQAAKLNSYQHFLRFGNAMLDKIA
                     SWRGELQLGHDVLFAPGAEAALDVRDPRGKLLLASHLGDVEVCRALAKIQGYKTINAL
                     VFSENAQRFKQIMQEMAPQAGINLMPVTDIGPETAILLKEKLDNGEWVAIVGDRIAVT
                     PQRGGDWRVCWSPFMGQPAPFPQGPFILASILRCPVNLIFALRQRGKLHIHCETFADP
                     LLLPRGERQQGLQNAIDHYAARLEHYALQSPLDWFNFFDFWQLPEIQDKE"
     gene            complement(11416..11769)
                     /locus_tag="ACHKBL_002856"
     CDS             complement(11416..11769)
                     /locus_tag="ACHKBL_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009008217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymyristoyl-ACP dehydratase"
                     /protein_id="HFO5819705.1"
                     /translation="MIRHEIERHQVQLQEMEIVLYLDPMLYWFNGHFAVQPLLPGVAQ
                     LDWVMHYATTLLAPGWRFRSIQNVKFLAPLIPETTVTLQLTWQETSQVLTFCYQRHDG
                     DARHTASSGKIRLCR"
     gene            complement(11756..13114)
                     /locus_tag="ACHKBL_002857"
     CDS             complement(11756..13114)
                     /locus_tag="ACHKBL_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HFO5819706.1"
                     /translation="MNQTLSLSQWLTASRPVTTPVAWLGEYTWTLGHLRHDVALLIDH
                     LRDQPGNRWALCFENSYLFIVALLATLHSGKIPVIPGHNRAALLNVQRSLFDCILSDK
                     RLGWNGPQFVVSTSHQMTTSTIAFDDIASDAFIELFTSGSTGQPKQIAKPVISLDQEA
                     NMLATHFADRLLGCRFVASVMPQHLYGLTFRIFLPMALGLPLHAAMLWYSEQLAALSH
                     TYRYAFVSSPAFLKRLDLHLTPPPVEMILSAGGMLPWQDVEKTSTWLNIWPDEIYGST
                     ETGILAWRYRQQDNIPWFTFSDVHLSQESEGVRVFSPLIPAEGLVLDDMLQFNENGQF
                     HLIGRRGRVVKIEEKRISLVEIEQRLLALDGIMDVAAIPLIRNGRQAIGVVVVPNKEA
                     RQIWQCSGGKTLELSWRKALLPWLEPVAVPRYWRIIDEIPVNNMNKRVYAQLQELFHD
                     TP"
     gene            complement(13111..13692)
                     /locus_tag="ACHKBL_002858"
     CDS             complement(13111..13692)
                     /locus_tag="ACHKBL_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5819707.1"
                     /translation="MSGIRSLPMIKLLTGLLLLAWPFLIWLGLAHNSLHWLSPLMALL
                     LLLRLRQPRRQAGPLQAVTKIVAVVGIVLCVSSFLLKTHQLLLFYPVVVNAVMLAVFG
                     GSLRSTMPIVERLARLQEPDLPEKAVRYTRRVTQVWCLFFIFNGSIALFTTLYGNMPL
                     WTAWNGIIAYLLIGFLTAGEWLIRCQMIKRKTP"
     gene            complement(13697..13948)
                     /locus_tag="ACHKBL_002859"
     CDS             complement(13697..13948)
                     /locus_tag="ACHKBL_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFO5819708.1"
                     /translation="MTDQQTIYQEVSALLVKLFEIDPQDIKPEARLYEDLELDSIDAV
                     DMIVHLQKKTGKKIKPEEFKAVRTVQDVVEAVKRLLQEG"
     gene            complement(13960..14217)
                     /locus_tag="ACHKBL_002860"
     CDS             complement(13960..14217)
                     /locus_tag="ACHKBL_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HFO5819709.1"
                     /translation="MQALYLEIKNLIISTLNLDELTPDDIDTNAPLFGDGLGLDSIDA
                     LELGLAVKNEYGVVLSAESEEMRQHFFSVATLASFIAAQRA"
     gene            complement(14192..15013)
                     /locus_tag="ACHKBL_002861"
     CDS             complement(14192..15013)
                     /locus_tag="ACHKBL_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312354.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HFO5819710.1"
                     /translation="MNKLMSRLGWAWRLVMTGLCFALFGLGGLLLSVVWFNILLVLVW
                     DTSRRRRLARRSIAASFRLFLTVAKGLGVLDYRIDGAEILRQERGCLVVANHPTLIDY
                     VLLASVMPETDCLVKSALLKNPFLGGVVRAADYLINSEAETLLPRCQQRLAQGDTILI
                     FPEGTRTKPGEKMTLQRGAANIAVRCASDLRIVTIGCSEHLLDKQSKWYDVPPARPFF
                     TVEVHGRVEINKFYDATSHEPALAARQLNRHLLLQLQQGCLPLSGINNASALSGN"
     gene            complement(15010..15732)
                     /locus_tag="ACHKBL_002862"
     CDS             complement(15010..15732)
                     /locus_tag="ACHKBL_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312353.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase chain length factor"
                     /protein_id="HFO5819711.1"
                     /translation="MKFALNITNWQALAPGLSDVQQWQAWSRQPWAIDPAAPLAKLSE
                     LPMMTARRLSSGSKLAVECGLAMLRRYQPDAVLYTSRHGELERNYRIVHALATEQALS
                     PTDFALSVHNSSVGNLTIAAKQPIVSSSLSAGRDSFQQGLCEVLSLLQAGYQRVLMVD
                     FDGFLPEFYHPQLPAEMPTWPYAVALMIESGDDWQCETQSAIAVNETTLPQSMLFLQH
                     YLQNAGAFSLSGERVQWRWSRR"
     gene            complement(15773..16831)
                     /locus_tag="ACHKBL_002863"
     CDS             complement(15773..16831)
                     /locus_tag="ACHKBL_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HFO5819712.1"
                     /translation="MYEQDTLSTLDAITEAQRIAFAPMLFQAALCLRNAGILDYLDQQ
                     GKQGAPLNAITEQTVLNEYAVSVLLDMGLSGRIITCKEGNYYLAKIGHYLLHDTMTRV
                     NMDFTQDVCYQGLFFLADSLNEGKPSGLKVFGDWPTIYPALSQLPDAARASWFAFDHY
                     YSDGAFNAALPYVFANNPTTLYDVGGNTGKWALRCCKYNENIAVTLLDLPQQIVLAKE
                     NIANAGFSDRIDFQAVDMLSDAPLPGEADIWWMSQFLDCFSPEQIISILSKIASVMKP
                     GAKVCIMELFWDAQRFEAASFSLNASSLYFTCMANGNSRFYSAEKFYDYLNKAGFQVA
                     ERHDNLGVGHTLLICQKK"
     gene            complement(16895..>17281)
                     /locus_tag="ACHKBL_002864"
     CDS             complement(16895..>17281)
                     /locus_tag="ACHKBL_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5819713.1"
                     /translation="KRYIKWLAITIFISMLSACARTAPVQQISTTVSVGHTQEQVKNA
                     ILKAGAQRKWIMTQVSPGVIKARYQTRNHVAEVRITYTATYYNIKYDNSLNLQASDGK
                     IHKNYNRWVRNLDKDIQVNLSTGATL"
BASE COUNT         4135 a         4570 c         4589 g         3987 t
ORIGIN      
        1 taacgcggtt cgatagagcc gttttcaatc aacggtcggc atgaaatcgc cacggcttcc
       61 cgccagtctt tgacctgttc cacgacctga acgacctctg tatttaatag attttttaac
      121 atgctactga taactcccga tgataattaa caggagagta gatattttac gcattaatta
      181 atgtgatgat tgtcgcattt gatagcgcta tctgataacg ctaccaatct ttgtgatagc
      241 gctatcattt ctctgccgcc atgctttggc tatattcggt tcctgcatat acaatgcaac
      301 aggatatttg ctaccgtggg caaaaactgg aaagagtaat ggtgaaataa aagtaatgaa
      361 aacagctggt tgtaattatc catggagtcg aaatggaaaa aacacgtaag cgccgtggta
      421 ccggacgggt gacgctgcag gaggtcgcta actttgcagg tgtcggaacc atgaccgttt
      481 cccgcgcgct gagaacaccg gaacaggttt ctgacaagct gagggaaaag atagaacagg
      541 ccgtagaaga gttgggttat attcccaaca gaactgctgg cgcccttgca tctggtcaca
      601 gtcatacagt cgccgtactg gttccttccc tgacagataa agccagctcc cgctttatgc
      661 agtcattaca gcaggtgctt aataaaaatg aattccagct actgcttggg tgtcatgagt
      721 ataatcagct caaagaagct gaaattctga tgacgcttct tcaaggcaac ccggcggcgt
      781 tagttatttt tggttctcag ttggctgata aaactcacca gatactcgag aaaacaaata
      841 tacctaccat taacgttatc ggttcccctt tcagtccggc tcaaattacc atcgaaacag
      901 cattttttga agcatcacat aaactgaccg aacatctgct ggagcagggt tataagaata
      961 tcggatttat cggtgcgcat atggacaacc gcctgcaacg tcagcaactg aacggctggc
     1021 ataaagcaat gctgagccat taccagaaca ctgatcttgt tatcaccatg ccggatgccg
     1081 caagtctgca attggggcgt tacgcattaa acgagatatt actgcgacaa ccagagctgg
     1141 atgctgtgat ttgcagccac gaagagattg cgctgggtat aatgtttgag tgccagcggc
     1201 gtttgttaaa aatccctgga aatatcgctg tagcttgtct tgacggttca gacaattgcg
     1261 atcaaacaca cccaactttg acatccatcc gtattgacta caaaaaaatg gggatagaga
     1321 caggtaagtt actgattgga ctattgaata ataatcatga tgaatcagaa gagtcgcgta
     1381 tcgtgcaatt caactaccag atagaattac gtcagagtac atgagcctaa aagagcgcta
     1441 ttacaaagtt agctattgtg gtagggatag ccgtttccgg ctattccccg cgcagttccg
     1501 gatgtatgct gctaacgcat ccattcctac ttcaggtatt tggcgtagaa gcggatgcta
     1561 aaaacttgtc gcaaaaccgc aatatcagcc tgcagtatcg ctctccagat tatccattcc
     1621 tatattgatg acaacgacag gtaagactgt gactcaccaa acttggatgt ttttctgagc
     1681 ttaaagctcg ttgcgtgaat aaggcgagag tcagcgaatc tcattgaggc ccgcagcacc
     1741 ggctgcaata aggatataga gcagcaatct accgtcaacg tcaaaaacac ggaagccttg
     1801 caaacgggat gcgttcatat aaacaacatg gagcagcaac agtacgatgc ctgttgttgc
     1861 tcagtcagga ccttttctgc catgcgcgag ccagcgtcac gattgataaa taatgttcgc
     1921 aactgattac tttactgcaa cagacgtggt tggtaataag cactggcgga caatgcagga
     1981 tggaaggctg ttttcaggtt gtccgttcag gtacgttaga tcagttcgtt gcgctaaaga
     2041 caactgttca gtcccttatg ttacccgtta tggcaaccat tgcccggtga taaccgggcg
     2101 tgtcatctgt gcatccgttg attcgtgtgg actgataaag ttttgtctgc tgaatcgtta
     2161 tacggaatgc tcttcagtat gcgtaagaga atttgaggtt catattcatt gattcagatt
     2221 ttttgaagaa tcaccacatg ggatgtccat gtggttgtat aactcagggc cagcgcttaa
     2281 taatgatgga tgtattaatg ccgccaaagg caaagttgtt actctgcaaa aactcacaat
     2341 caaccttgcg ggcttcatgc atgatgtaat ccagcgcgcc acaattcggg tctggtttat
     2401 tcaggtttag cgtaggcgca aaccaacctt cgcgcatcat ttgcagactc atccaggctt
     2461 cgagcgcgcc acaggcacca agcgtatgac caaaataact tttcagcgag gagagcggca
     2521 cattatcacc gtaaatggtt gctgtcgcca gactttctgc catatcaccg cgatcggtcg
     2581 ccgtaccatg cgctgaaata tagccgatat cttgtgcgct taaccctgcg atttttagcg
     2641 attgctccat acaatactgc atggtttcgc gctgcggctg ggtaatatgt gccgcgtcgc
     2701 agttagtcgc aaagccgacg atttcaccgt aaatggtcgc gctgcgcgct ttggcatgct
     2761 ccagttcttc gagaatgagc gtacctgcgc cttcaccaat caccagtccg tcgcggtttt
     2821 catcgaacgg tgaaggtgtg gttttcggcg catcattgtg ctggctggtg gcaaaaaggg
     2881 tatcaaacac cgctgcttct gaagggcata actcttcggc accaccggca accatcaccg
     2941 tttgataacc gtggcgaatg gcttcccacg cataaccgat cgcctggctg ccggaggtac
     3001 aggcgctgga ggttgggatc acccgcccgc gcaggccgaa aaagaggcca gtattaacag
     3061 cggtggtgtg cggcatcatc tgcacatagg tggtgccggt gatgttgttc gtgtgctttt
     3121 cagtcagcat ggtggcaaat tcgctcaccg gaccggtact gccggtcgat gaaccgtacg
     3181 caatgcccgt ttcgccgctg gttaatatcg gatcgccaat caaccctgcc tgctctaacg
     3241 ccagctcgct ggcacgggtc gacatttgcg acacgcggcc catcgcacgg atacgcttgc
     3301 gggtgtagtg ctccggtagc gtgaaatcat ccacaggcgc gccaagcagg gtgtgcagcc
     3361 catcgtagac ctgccactcc ggcattttgc gcaccgcatt ttcatacgcc agtaagcgtg
     3421 cagaaacgtc ctgccagttt tcaccaaagg cagtcacgcc gcccattcct gtaatcacta
     3481 cgcgacgtgt cataacatcc ctccgttgat ggaaataacc tggcgggtga cgtaacccgc
     3541 aatatcagac attaaataac tggcaagccc ggcaacttct tccgcctggc ccatacgttt
     3601 catggggatc atcgacattg cctctttcag agcgctttct tccatttcga tcatcccggt
     3661 atcaataagc cctggcgcga tgcaattgac ggtgattttg cgctttgcga gttcgatagc
     3721 cagtgctttt gttgcgccga taattccggc tttggctgcg ctgtaattca cctggccgcg
     3781 attgcccatt acgccggaaa ccgaggacag cgtgatgatg cgcccaccct ggcgcgcgcc
     3841 gatcattggc ataatgcacg gctgaatgac gttgtagaaa ctgtcgaggt tggtgtggat
     3901 caccgcatcc cagtcgtcat cgcttaaagc cggaaaagcg gcatcgcggg cgatcccggc
     3961 gttactgacc acgccgtacc aggcaccgtg ttgcgcgatc tcatgctcca gcacttcccg
     4021 acactgttcg cggtttgcga cgtcgaaact gagcaaacgc ccgttacccc cgttggcgac
     4081 aatggcattc agcgtttcct gagcgcctgt ggcgtcacgg tgatagtgaa cgccaatgtt
     4141 aaagccgtct gccgcaagct tgcaggcgat ggcgcgacca atgcctttgc tggcaccagt
     4201 aaccagaact gaacgactca tcaggacgct ccttgttgaa acagggtggt aagttcttcc
     4261 gcggtcggct ggaaggtgtt aatgcggccc gtcgcctcgc cgtcgttgat gctgcactca
     4321 aagctgccga agcgatcgtc ctgcatcagc agtttgacgg taatggtcag cgtctggcct
     4381 gcgggcagaa tgccagtggt gcaaagcaat tcacgcgcgc ccagtatcat ccctaactca
     4441 atactgtttt tgccctgctg gtggcgatgc cagccagacc agacgccgac ggtttgcgcc
     4501 atcagttcaa gggcaaacca gccagggaga ttgccgtccg ggtcgagaaa cggtgccagt
     4561 acgccactgg gcgaaaccgt cacgcggcac accgcgctgt catcgctgac gctcaccacg
     4621 tcttccagca gtaacatggg cgcgtcgtgg ggcagataag cgccggggga taaatagtgg
     4681 ctcacgaaac cctcccgagc agaatgctgg cgttattgcc gccaaaggcg aaggaattgg
     4741 acaggatcac cggacgtgcc agcggctgcg gtttttcgat aatgccacac ggtggcagcg
     4801 tgggatcgcg cggcgacagg ctgaagtcct gagcgggcag cggtaaatca cgctgtaaaa
     4861 tcaacatact aattgccgcc tcggtaatgc cagccgcgcc gagcgtgtgt ccggtcaaat
     4921 gtttggtgga gctgcacggt atgcgtttgc caaacagggc gttaaccacc attgattcaa
     4981 tctggtcgtt gagttgtgtg gcggtaccgt gcaggttgat atagccgatg tcatctggcg
     5041 taagctgcgc atcggtcagc gcctggttaa tggcgcggat cgccccttcg ccttgcggat
     5101 gcggggccga aatatggtaa gcgtcgctcg attcaccaac gcccagtaac gcgatgggct
     5161 gcggttcacg ggttaataac atcagtcccg ccccttcgcc aatggtgata cctgcgcggt
     5221 cacgaccaaa cggctgacat aaggttggcg agagcgattc gaggctgtga aaaccgttaa
     5281 tcggcatccg gctcagagta tctgcgcccc ccacaatggc ggcatcgacc agccccgctt
     5341 cgataagcct gcgcccgctg atcatcgctc tggcgctgga agagcaggcg gttgaaaggg
     5401 tataggccgg gccgtcgagc gccagccagt ggctgagaaa acgcgacgga tcgccgagtt
     5461 cctgttgcgc gtagtgccat gtggtgctcg gctgtccgtt ggtgcgcagg ttaacgtgcg
     5521 tgtcgccctc atgcaggccg gaggtgctgg tgccaagcac aatcgcgatg cgctcgcggc
     5581 catatttcgc gatagcgtca tcaacctgcg gctggatctg cgccagcgcc gccagcagga
     5641 tctgattgtt gcgcgagcgg tgcgcagcga atttttcggg gataagcgga agctcgccat
     5701 ccacgccagc caggaccgcc tgcggatgcc cctgcaacca gcctgcgcgc ggacgcatgc
     5761 cgggggcgac accgcgagcc aggttggctg cgatttcgtc aagattattt cccagggcgt
     5821 tgatcatgcc gacggcggaa atataaatca tatcagtcac ctaagtattg aatggtgatg
     5881 tggtatttaa agacatgctg ctcaatgctg attggcacgc gtttgccctg gcgattcaga
     5941 tagatgattt ccgtgaccag cttgccgctg gcgttacgca gctcgcgttt gttgccgttg
     6001 tcgcgaagcg tccagccagt gggaagttgc ggttgccagg cgctaatcgg ccagtggctg
     6061 agcattacgt cagccagcac ctgacttgcg ggcggcagct gcgggacgac gatggattgc
     6121 tcggcgcgta gaccttttgc atcgtaggtc accagaaaca ggcgaatgcc gaccgacgac
     6181 agcccggcaa gggtgatttt ctggtcgtcg gcgttaagca tcactagcag cgactgggtt
     6241 ttgccgttga agctgccgct caagagttgc tgggaattga cggcgggtga aatccccggc
     6301 gcaggcagcg tgatgcgtgt gccaggttgc agccacgcct gcgggcggcc ttcctgttcc
     6361 ggttgcgagt ggctacagcc ggtgaggata agcgtagcgg tcagggcgag cgctcgccag
     6421 aaggtggatt tgatcatttt tttgttctct ttttatcggg catagccagc ggcgaaagca
     6481 ggaaggcggt gaaaataccg ctcaccagca caataccaaa actgctgatg gcctgggtgg
     6541 cgctgaatac cagcattccc agcgtcagca aggtggtgag cattgccagc gtgatcgcca
     6601 gtagggaagt taacggtgta ccgcgcggat tactgaaaaa cagcgtgtaa ttgatgccga
     6661 tgccaagcac cagcaccagc gccagtagcg aaaagagatt caccgcctgc ccgctcatcg
     6721 ccagcactgc cagaccgcag cccagcgaaa gcaccgaagg caccaggctg ataagccctt
     6781 tgcgccagcc gaggcgggcc actgcgccgc aggcaattac tgccagcgcc accagcaaca
     6841 agccggttaa cacataacgg taaagtgcga acaattcatc aaaggtgctt ttgcgatcaa
     6901 cccaggcaat gccgccaggg taatagctgg cgatttcctg ctgcaacgcg ctacttttaa
     6961 ccccttcaac cggcaccagt acgccgcttt cgccgttttc cagcgtcagc cacagcagac
     7021 gccagccttc actggcgggg cttgccagcc attcatcaac cttcactggc atggcgttaa
     7081 gatccgggtt caccgccgtc agcccggcat tttgcagtgc ttttgttacc gtcggggcag
     7141 ccgttttcag caggtctaaa tcttcctcct gccgcgccag ggagttcagc ggaatggtgc
     7201 ggtagttgct gataaggccg tcttttttcg ctttctccag cgaggtggta aatgtctcca
     7261 gccgtcccaa ggtttgctgt ggggagtcgc cgtaaactac aaaccatttt tgatcgacgc
     7321 tttgtccggt cagggcggta atggctttct cctgcgccag aatatgctgc ggcagcgcct
     7381 gtaactgcga gatatcgtca tcgacgcgta gcattgacat ccccgccagc gaaaacagcg
     7441 ccagcgcgac gggcagaccc agcgacagtt ttttattacg ccgccacgcc gccagccagc
     7501 gcagcatcag cgccatcgcc ggaaccgggc gtaccggcag gccacggcac agccacggat
     7561 gccagaacag gacggtcaga caggaggcgc tcaacccgac ggcggcaaaa atcgccattt
     7621 gccgaatacc gggaaaaggg gcgagcatca taatcagata tgccgccacg gtggtgagca
     7681 gcgccagtag caaggcattg cgcactttcg ccaggctttg ccacggcgaa acgtcgttgc
     7741 cgtgcaccat ccgttcggtg aggtaataga gcgtgtagtc agcggaaatg ccgataacgc
     7801 tcatgctcat caccagcgtc atcaggtgta attcgccgaa aatcaacaat gtggcggccg
     7861 ttcccgccag cgcgccgatg ccgatggaaa tcacgcaaag cagcaacggg cgtaaagagc
     7921 ggaacaccgc cacaatcatc aatatcaccc ccagcagcgt tgccacgccc agggtggaga
     7981 tatcctgctt cgcctgttgg ctggcgtaat cgctgtaaaa caccgtgccg cgcgagagca
     8041 actgcgcctg cgggtaacgc gttttcagat ctttttccag cgtattcagg gtcgtgatga
     8101 gctggtgggt ttgctgcata tcaaacgacg atcccgccag ttcgccgtgc agcagatacc
     8161 agtagttgcc ctggggatcc tgcgtcacca gccagccgtc catcagtcgt aaacgctggc
     8221 cgtttttcgc catcgccagc tgcgagccgc gcattaacat cagcggatcg ttttgcaact
     8281 ccttgccgct taccccggag aacgcagaat aaagctggga gaggatccat tgtgcctgcg
     8341 cttcgccgcc gttttgcagg cgggcgcggg tgtcggggtc aatcaggccg ttgcggtgct
     8401 gccagaaaaa cgccccccac gcctgctggc tggcggcatc cattggccct ttaatgtcgc
     8461 caagcgcagc ggacttttgc agcagcgtca gccattcctg agccaccctg ggattagcct
     8521 ctttaccggg gctgaccagc cacaccagtt ggcggtccag acgctgcata aagccgtcat
     8581 tcagcgctgg gggaatatcg cccatcgcct gtttgggtaa catagccagc acgctactgt
     8641 tcagccgtga ttgcggcagc aggatcagca ataccaccgc catgaccagg cagactagcc
     8701 cccataacag cgcggggcgt ttactgggcg gcaaaacgtt ggcgttcgtc atcgctcagt
     8761 tgcgctggcg tcagttgatg ttgggtaaga gcgatatcgg tgcgatcgcc ctgtttatca
     8821 ttaagctgaa tgctctccag ataggtttgc ccggcgagat cgatggtgtt gaaaatttta
     8881 tccagcggcg tggtggtcgg cgtcaggcgc agcgtccagc ggccttcgcc tttatcggca
     8941 aattcgacgc ggaagttttg ttccagcact ttgcgatcgg cctggaacag cgcgcgcagc
     9001 aggtggttaa actggaacat ctgcgggtta ttttctgccg tgatgatttg cggcggctgg
     9061 ccgttgatca cctgcaccat gcgtttatcg tcgagcaata gctgcatggg gaatggtgaa
     9121 gtttgatccc acaacagccc ctggtcgcgg gcgatcaaca tctgaccctg agatcgcagc
     9181 ggctgcggca gatctttaat cgtccgggtt tgatcgaaat gggcgcggat caccggttgt
     9241 tcggtaaagc gttgctgaag atcgtccagg gtcagggcgc tcacaaacgg gctaatcagc
     9301 agcgccagca gcggtaaaaa tttcatggtt tgactcccat gcgttcaaac agaatatcgg
     9361 ggctgacaaa gcacagttca cggctttgct cctctactgc gacctgaatg gtgtatcctg
     9421 tggtggcgcg tttgcccgtt tcggcatcga aaatctgata gccaatccgc agacggtttt
     9481 cgaactcttc gatatgcgca cgcacgcgga tgcgttgctc aaaggtgagg gcgtggcgat
     9541 atttcacccg cgcatcgacc actggccaca aataacctga ctctttcatc tgccgatagc
     9601 cgtaattgaa ctggttgagc agcgcctcgc gggccacttc aaagtagcga aaatagttac
     9661 cgtgccaggc cacgcccatc atatcgacgt cgtgaaaggg aatggtcagc tcgacttcag
     9721 cggtaaatcg gggatcgtta agcacccttt actccttgtc ctgaatttcc ggcagttgcc
     9781 agaaatcgaa aaaattaaac cagtcgaggg gcgactggag cgcgtaatgt tccagacgcg
     9841 cggcgtagtg gtcgatagcg ttttgcagcc cctgttggcg ttcgccacgc ggcaacagca
     9901 gcgggtcggc aaaggtttcg cagtgaatat gcagcttgcc gcgctggcgc agggcgaaaa
     9961 tcagattcac cgggcagcgc aaaatagacg cgagaataaa cggcccctgt gggaaaggcg
    10021 caggctggcc cataaacgga ctccagcaga cgcgccagtc gccgccgcgt tgcggggtga
    10081 cggcgatgcg gtcaccgaca atcgccaccc attcgccgtt atccagcttc tctttcagca
    10141 ggatggcggt ttccgggccg atatctgtta ccggcatcag gttaatgcct gcctggggag
    10201 ccatctcctg cattatctgt ttaaagcgtt gggcgttttc gctaaacacc agcgcgttaa
    10261 tggttttgta gccctgaatt tttgccagcg cccggcacac ttccacatcg ccaagatgcg
    10321 aggccagcag caatttgccg cgcggatcgc ggacgtcaag cgccgcttct gcgcctggcg
    10381 caaacagcac atcgtgcccc agttgtagct cgccgcgcca gctggcgatt ttgtcgagca
    10441 tggcattacc gaaacgtaga aagtgctgat agctgttgag ttttgccgcc tgtggttgat
    10501 gctgtcgtac acgggcgagc cagtcttgcg acgctttgcg cgcacgagca gcagtgagcc
    10561 agtagacgcc caccaccggg taaagcagcg cgctaaacgc cgttcttccc agcagacgcc
    10621 agaccagcag catcaggcgc attccccata atcctttcac ttcactctgc cgcgcccagt
    10681 ggctggaaga gcggcgcatc agtagtgaag gaatacgcgg caacatgccg aaaaacagac
    10741 gcgtgtgcat gagcgagatg cggacgttat ctttcagggc atcaaaatgc gaaagcccgt
    10801 cctgcggata agtgacgcgg gttggcacga aataacaggt gtttccctgc cagtagaggc
    10861 gcaccatcac ttcagtgtcg aaatccatcc gcttgccgat ggtggcgtat tttgccagtt
    10921 gcagcgttgg cgcaaccgga taaacgcgaa agccgcacat actgtctttc agttgcaggg
    10981 agagcgtttc gatccatacc cagacgtggg tgacccagcg cccgtaaagc cgtgagcggg
    11041 ggatggagtc atcgtaaatt ggctggccgg agatcagcgc cgcagggtgt tgttcagcga
    11101 gtgccagcag tttggggata tcttcaatcg catgttgacc gtcagcatcc acctgcaccg
    11161 catggctgaa gcccgcgtct gccgccgcct gtaagccgcg cattaccgcc gcgcctttgc
    11221 ctgtgttttc tgccaggcga attaaggtca cgccaggctg ttcggcaacc agattatcca
    11281 gttgctgttg cgtggcggca tcgctgccgt catccaccac aatacaggga agattaaatg
    11341 gcttaagacg cgccagcacg cccggcatca tcgcaccgtg gttgtagcag gggatcaaca
    11401 cgcagggaga aaagtttacc gacataggcg gatcttcccg ctactggcgg tgtggcgggc
    11461 gtcaccgtcg tgacgctgat agcagaaagt gagtacctga cttgtttcct gccatgttaa
    11521 ttgcagggta acagtcgtct caggtataag tggcgccaga aatttaacat tctggatact
    11581 acgaaaacgc cagcccggag caagcagcgt ggtagcataa tgcattaccc agtccaactg
    11641 ggccacgcct ggtaacagag gctgtacggc aaaatgacca ttaaaccagt agagcatcgg
    11701 gtcaaggtat agaacgattt ccatctcctg cagttgaacc tgatggcgct caatttcatg
    11761 gcgtatcatg aaataactcc tgtaattgtg catagacacg cttattcata ttgtttactg
    11821 gtatttcatc aataatgcgc cagtaacgag gtactgcaac aggttcaagc catggtaata
    11881 gtgctttgcg ccaggacaac tccagagttt tccctccgga gcattgccat atttgacggg
    11941 cctctttatt tggtacaacc acgacaccga tagcctggcg gccattgcgt attaatggta
    12001 ttgcagcgac atccataata ccgtcaagtg ccagaagccg ttgttcgatt tcaaccagtg
    12061 atatccgctt ctcctctatt tttaccactc gaccacgtcg tccgatcagg tggaactgac
    12121 cgttttcatt gaattgcaac atatcgtcaa gcaccaatcc ttcagcaggg atgagtggcg
    12181 aaaagacacg aaccccctca ctttcctgtg aaagatgaac atcagaaaaa gtaaaccagg
    12241 ggatattatc ctgttgacgg taacgccatg cgagaatacc tgtttcggta ctaccataga
    12301 tttcatcggg ccagatattc agccaggtgg aagttttctc tacatcctgc catggtagca
    12361 taccgccggc agagagaatc atttccacgg ggggaggcgt cagatgaagg tcgagtcgtt
    12421 taaggaacgc cgggctgctg acaaaggcat agcggtaggt atggcttaat gcggcaagtt
    12481 gctcagaata ccagagcatt gcggcatgca atggcaggcc aagtgccata ggtaaaaaaa
    12541 tacggaaagt cagcccatac aggtgctgag gcatcacgga tgctacaaaa cgacagccca
    12601 gtagtcggtc tgcaaagtga gttgccagca tgtttgcttc ttgatcaagg ctgataacag
    12661 gttttgcaat ttgttttggt tgtccggttg aacccgatgt gaaaagttca ataaacgcgt
    12721 cagaagcaat atcatcaaag gctatcgtgc tggtcgtcat ctggtgagat gtgcttacaa
    12781 caaactgtgg accgttccag cccagccttt tatcactcag gatgcaatca aacaaggagc
    12841 gttgtacatt taacaaagcg gcgcgattat ggcccgggat tactgggatt ttacctgagt
    12901 gtagcgtggc caacaacgct acaataaaaa gatagctgtt ttcaaaacaa agtgcccagc
    12961 gattgcctgg ctggtcgcgt aaatggtcga tcaaaagcgc tacatcatgc cgcagatgcc
    13021 ccagtgtcca ggtatattcc ccaagccagg caacaggagt tgttacaggg cgagaggctg
    13081 tcaaccactg tgaaagtgag agagtctgat tcatggtgtt ttccgtttga tcatctgaca
    13141 acgtatcagc cattcccctg cagtcaggaa acctatcaga aggtaagcaa tgataccgtt
    13201 ccaggctgtc cacaaaggca tgttgccata caaggtggta aataacgcaa tactgccgtt
    13261 gaaaataaaa aacaggcacc agacctgcgt tacgcggcgg gtatatcgca cggccttttc
    13321 cgggaggtct ggttcctgca aacgtgccag ccgctcaact atcggcattg tcgaccgcag
    13381 cgaaccgcca aaaaccgcca gcatgacagc gttaaccact accgggtaaa acagcagtaa
    13441 ctggtgcgtt ttcaacagaa aactggagac acacaataca atgcccacaa cagcgacgat
    13501 ttttgtgacg gcttgtagcg gccctgcctg acgacggggc tggcgcaggc gcaataaaag
    13561 cagcaacgcc atcagcggcg acagccagtg caggctgtta tgcgccaaac cgagccagat
    13621 taaaaaaggc caggcgagca gcaataatcc ggtcagcaac ttaatcatcg gtagcgagcg
    13681 aatgcccgac atcaaattag ccttcttgta gcaggcgttt tacagcctct acaacatcct
    13741 ggactgtacg cactgcttta aactcttccg gcttgatttt cttaccggtt ttcttttgca
    13801 ggtgcacaat catgtcaacg gcgtcgatgc tgtccagttc cagatcttcg tacaggcgcg
    13861 cttcaggttt gatgtcctgc ggatcgattt caaacagctt aaccagcagg gctgagactt
    13921 cctgataaat ggtttgttgg tctgtcatga tggctttcct caggcacgtt gcgcagcaat
    13981 gaaagaggcc agagtggcga cggagaaaaa gtgctgacgc atttcttcgc tttccgcaga
    14041 gagcacgacg ccgtactcat tttttactgc cagccccagt tccagtgcat cgatagagtc
    14101 cagccctaaa ccgtcgccaa acagtggcgc attggtatca atatcatcag gagtcaactc
    14161 gtccagattg agggtgctga tgatgagatt tttaatttcc agataaagcg cttgcattat
    14221 ttattcctga gagaggtaaa caaccttgtt gtaattgaag caaaagatgt cggtttaact
    14281 gccttgcagc cagcgccggc tcatgtgaag ttgcatcgta aaatttattg atttctaccc
    14341 gcccatggac ttcaaccgta aaaaacggcc tggcaggcgg aacgtcatac catttacttt
    14401 gtttatccag cagatgctca ctacagccga ttgtcacgat gcgtaaatcg ctggcgcagc
    14461 gcacggcaat attcgctgca ccgcgctgta atgtcatttt ttcaccgggt ttcgttcgtg
    14521 tgccttcggg gaaaatcagg atagtgtcac cctgtgccag tcgctgttgg cagcggggta
    14581 acagggtttc agcttcgctg ttgattaaat aatctgccgc ccgcacgacg ccgccaagaa
    14641 aaggattttt taacagcgcg cttttcacca ggcagtcagt ttctggcatg actgacgcca
    14701 gcagcacgta atcgatcagc gtgggatgat tggctacgac gaggcaaccg cgttcctgac
    14761 gtaaaatttc agcgccatca atacgataat ccagcacgcc aagccctttc gcgacagtta
    14821 aaaacaggcg aaaactggcg gcaatgctgc gacgcgccaa acgacggcgg cgggaagtat
    14881 cccataccaa aactaacaat atgttaaacc atacgactga aagcaacagg ccgccgagac
    14941 caaacagggc aaaacataac ccggtcatga ccagtcgcca tgcccatccc aggcgcgaca
    15001 tcagcttatt catctgcggc tccagcgcca ttgcacgcgc tcgccagaca gtgaaaatgc
    15061 gcctgcattt tgtaaatagt gctgtaaaaa caacatgctt tgcggcagtg ttgtttcatt
    15121 taccgcaatg gctgactgcg tttcacattg ccagtcatcg ccagattcaa tcatcagcgc
    15181 gacggcgtac ggccaggtcg gcatttctgc aggcagttgt gggtgataaa actcgggcag
    15241 aaaaccgtca aaatcgacca tcaacacgcg ctggtatccc gcttgtagca ggctaagcac
    15301 ttcacacaaa ccttgctgga aactgtcgcg accagctgaa agcgatgacg aaacgatcgg
    15361 ctgtttggcc gcaatggtca ggttacccac ggaagaattg tgtacggaaa gcgcgaagtc
    15421 cgtcggagaa agcgcctgtt cggttgccag tgcatgaaca atgcggtaat tacgctccag
    15481 ttcgccatga cgactggtat acaaaacggc gtcaggctga tagcggcgta gcattgccag
    15541 accgcattca acagccagtt tgctgcctga actgagacga cgggcagtca tcattggcag
    15601 ttcacttaat tttgccagcg gcgcggcagg gtcaatcgcc cacggctggc gcgaccatgc
    15661 ttgccattgc tgcacatcgc taagtccagg cgccagcgcc tgccagttgg taatatttaa
    15721 tgcaaatttc atcaacgcgt gcccgcggta aaccaaaatt attaagtgac gattatttct
    15781 tttggcatat caaaagtgta tgtccaacgc ctaaattatc gtggcgctct gctacctgga
    15841 atcctgcttt attcagatag tcgtaaaatt tctctgcact atagaaacgg ctgttaccgt
    15901 ttgccatgca ggtaaaataa agtgaggacg cgttcaggct aaacgatgct gcttcaaacc
    15961 gctgcgcatc ccagaacaac tccatgatgc aaacttttgc gccaggcttc attacgctgg
    16021 cgattttgct gagtatgcta attatttgtt cgggtgaaaa acagtctaaa aattggctca
    16081 tccaccagat atcagcctcg cctggtaacg gggcatcgct tagcatatcc accgcttgga
    16141 aatctatgcg atcagaaaaa cctgcattcg cgatattttc tttcgccagt acgatttgtt
    16201 gtggcagatc taataacgta acggcgatgt tttcattgta tttgcagcag cgcaaagccc
    16261 acttacccgt attgccgccc acatcgtaca aggttgtggg gttgttggcg aatacataag
    16321 gcagtgcggc gttaaatgcg ccatctgaat agtaatgatc aaatgcaaac cagctagcac
    16381 gagcagcatc tggtaattgc gaaagtgccg gatatatcgt cggccagtcg ccaaatactt
    16441 ttaacccgga aggttttcct tcattgagtg aatcggcaag gaagaaaagc ccctggtaac
    16501 agacatcctg ggtgaaatcc atattcacgc gagtcatagt gtcatgcagg aggtaatgac
    16561 cgattttcgc caggtaataa tttccctctt tacaggtaat aattcgcccg cttaacccca
    16621 tatccagtaa cacactcacg gcatattcat tcaggactgt ctgttcagta atcgcgttaa
    16681 ggggagctcc ctgtttaccc tgttgatcca aataatctaa tattcctgca ttgcgcaggc
    16741 acaaagctgc ctggaaaagc ataggagcaa aagcaatccg ttgtgcctcc gtgatggcat
    16801 ccagtgtgct gagtgtgtcc tgttcgtaca tatataacca ttcttataga gaagtttaat
    16861 gatatcgggc tattccagcc cgatatcagc atcactataa cgttgctcct gtagacaagt
    16921 taacctgtat atctttatcc aggttacgca cccaacggtt atagttttta tgaatttttc
    16981 catcagaagc ctgcagattc aggctattgt catatttgat gttatagtaa gtagctgtat
    17041 atgtaatgcg aacctcagca acgtgatttc gtgtttgata gcgagcttta ataactccag
    17101 gggacacttg cgtcataatc cacttgcgct gtgcacctgc tttcaaaatg gcatttttaa
    17161 cctgctcctg agtatgaccc acactgacag tggtgcttat ctgttgcact ggagccgtac
    17221 gggcacaggc actcaacata ctgataaaaa ttgtgatggc aagccatttt atataacgtt
    17281 t
//
LOCUS       DAZFLD010000105        17156 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.106, whole genome shotgun sequence.
ACCESSION   DAZFLD010000105 DAZFLD010000000
VERSION     DAZFLD010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17156)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17156)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17156
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.106"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            503..1591
                     /gene="pdeL"
                     /locus_tag="ACHKBL_002865"
     CDS             503..1591
                     /gene="pdeL"
                     /locus_tag="ACHKBL_002865"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator/c-di-GMP
                     phosphodiesterase PdeL"
                     /protein_id="HFO5819714.1"
                     /translation="MNSCDFRVFLQEFGTTVHLSLPGSVSEKERLLLKLLMQGMSVTE
                     ISQYRNRSAKTISHQKKQLFEKLGIQSDITFWRDIFFQYNPEIISATGNNSHKYINDN
                     HYHHIVTPEAISLALENHEFKPWIQPVFCAQTGVLTGCEVLVRWEHPQTGIIPPDQFI
                     PLAESSGLIVIMTRQLMKQTADILMPVKHLLPDNFHIGINVSAGCFLAAGFEKECLNL
                     VKKLGNDKIKLVLELTERNPIPVTPEARAIFDSLHQHNITFALDDFGTGYATYRYLQA
                     FPVDFIKIDKSFVQMASVDEISGHIVDNIVELARKPGLSIVAEGVETQEQADLMIGKG
                     VHFLQGYLYSPPVPGNKFISEWVMKAGG"
     gene            complement(1633..2565)
                     /gene="yahB"
                     /locus_tag="ACHKBL_002866"
     CDS             complement(1633..2565)
                     /gene="yahB"
                     /locus_tag="ACHKBL_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFO5819715.1"
                     /translation="MNSIFTEENLLAFTTAARFGSFSKAAEELGLTTSAISYTIKRME
                     TGLDVVLFTRSTRSIELTESGRYFFRKATDLLNDFHAIKRSIDTISQGIEARVRICIN
                     QLLYTPKHTARLLQVLKKQFPTCQITVTTEVYNGVWDAIINNQANIAIGAPDTLLDGG
                     GIDYTEIGAIRWAFAIAPDHPLAFVPEPIAESQLRLYPNIMVEDTAHTINKKVGWLLH
                     GQESILVPDFNTKCQCQILGEGIGFLPDYMVREAMAQSLLVTRQIHNPRQDSRMLLAT
                     QHSATGQVTQWIKKQFSPNGILTGIYQDLLHREN"
     gene            complement(2657..3154)
                     /gene="yahC"
                     /locus_tag="ACHKBL_002867"
     CDS             complement(2657..3154)
                     /gene="yahC"
                     /locus_tag="ACHKBL_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HFO5819716.1"
                     /translation="MNGLTATGVTVGICAGLWQLVSSHVGLSQGWELLGTIGFVAFCS
                     FYAAGGGKSGFIRSLAVNYFGMVWAFFAALAAGWLASVSGISGFWASVIMTVPFSAVV
                     VWQGRFGLLSFIPGGFLGMTLFFASGMNWTVTLLGFLAGNCVGIISEYGGQKLSEATT
                     KRDGY"
     gene            3412..4017
                     /gene="yahD"
                     /locus_tag="ACHKBL_002868"
     CDS             3412..4017
                     /gene="yahD"
                     /locus_tag="ACHKBL_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HFO5819717.1"
                     /translation="MTIKNLPADYLLAAQQGDIDKVKTCLALGVDINTCDRQGKTAIT
                     LASLYQQYACVQALIDAGADINKQDHTCLNPFLISCLNDDLTLLRIILPAKPDLNCVT
                     RFGGVGLTPACEKGHLNIVKELLAHTEINVNQTNHVGWTPLLEAIVLNDGGIKQQAIV
                     QLLLEHGASPHMTDKYGKTPLELARERGFEEIAQLLIAAGA"
     gene            4057..4920
                     /gene="yahE"
                     /locus_tag="ACHKBL_002869"
     CDS             4057..4920
                     /gene="yahE"
                     /locus_tag="ACHKBL_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HFO5819718.1"
                     /translation="MWALTADADFLAQRGQGQVEQVFARAVNIALPARQQLLTLLCEE
                     YDNAPNSCRLALTHFDGLFRHGDKVQFDDQGITVGQHLHIEMSRCRRWLSPTLQMAAV
                     NFHLIAWQQWHDIIHQHLGENETLFNYCGDNPFYQALNKELHIKRRAVIQAVNDKQNI
                     ASAVASMMGLGIGLTPSADDYLTGLALILFISGHPAEKYKEEFYLGLQCGRNNTTLLS
                     AITLEAALQQRCRENIHRFIHNIIYDIPGNATQAIEKIKHIGSSSGCDMLYGMADGCA
                     LSQTYGGNYVS"
     gene            4910..6457
                     /gene="fdrA"
                     /locus_tag="ACHKBL_002870"
     CDS             4910..6457
                     /gene="fdrA"
                     /locus_tag="ACHKBL_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HFO5819719.1"
                     /translation="MSVKIVIKPNTYFDSVSLMSISTRANKLDGVEQAFVAMATEMNK
                     GVLRNLGLLTPELEQAKNGDLMIVINGKSGADNEQLLVEIEELFNSKAQSGSHEARYA
                     TIASAKKHIPESNLAVISVNGLFAAREARQALQNDLNVMLFSDNVSVEDELALKQLAH
                     EKGLLMMGPDCGTAIINGAALCFGNAVRRGNIGIVGASGTGSQELSVRIHEFGGGVSQ
                     LIGTGGRDLSEKIGGLMMLDAIGMLENDPQTEIIVLISKPPAPAVARKVLERARACRK
                     PVVVCFLGRGETPVDEQGLQFARGTKEAALKAVMLSGMKQEHLDLHTLNQPLIADVRA
                     RMQPQQKYIRGLFCGGTLCDETMFAVMEKHGDVYSNIQPDPEFRLQDINRSVKHTFLD
                     FGDDDFTNGKPHPMIDPTNRISRLIEEARDPEVAVIVMDFVLGFGSHEDPVGSTIEAI
                     KEAKAIAAAEGRELIILAYVLGTDLDTPSLEQQSQMLLDAGVILASSSTNTGLLAREF
                     ICKGEEA"
     gene            6457..7875
                     /gene="yahG"
                     /locus_tag="ACHKBL_002871"
     CDS             6457..7875
                     /gene="yahG"
                     /locus_tag="ACHKBL_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HFO5819720.1"
                     /translation="MSPSLFSQPLNVINVGIAMFSDDLKKQHVEVTQLDWTPPGQGNM
                     QVVQALDNIADSPLADKIAAANQQALERIIQSHPVLIGFDQAINVVPGMTPKTILHAG
                     PPITWEKMCGAMKGAVTGALVFEGLAKDLDEAAELAASGEITFSPCHEHDCVGSMAGV
                     TSASMFMHIVKNKTYGNIAYTNMSEQMAKILRMGANDQSVIDRLNWMRDVQGPILRDA
                     MKIIGEIDLRLMLAQALHMGDECHNRNNAGTTLLIQALTPGIIQAGYSVEQQREVFEF
                     VASSDYFSGPTWMAMCKAAMDAAHGIEYSTVVTTMARNGVEFGLRVSGLPGQWFTGPA
                     QQVIGPMFAGYKPEDSGLDIGDSAITETYGIGGFAMATAPAIVALVGGTVEEAIDFSR
                     QMREITLGENPNVTIPLLGFMGVPTAIDITRVGSSGILPVINTAIAHKDAGVGMIGAG
                     IVHPPFACFEKAILGWCERYGV"
     gene            7901..8155
                     /locus_tag="ACHKBL_002872"
                     /pseudo
     CDS             7901..8155
                     /locus_tag="ACHKBL_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            8205..9155
                     /gene="yahI"
                     /locus_tag="ACHKBL_002873"
     CDS             8205..9155
                     /gene="yahI"
                     /locus_tag="ACHKBL_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase family protein"
                     /protein_id="HFO5819721.1"
                     /translation="MKELVVVAIGGNSIIKDNASQSIEHQAEAVKAVADTVLEMLASD
                     YDIVLTHGNGPQVGLDLRRAEIAHEREGLPLTPLANCVADTQGGIGYLIQQALNNRLA
                     RHGEKKAVTVVTQVEVDKNDPGFAHPTKPIGVFFSESQRDELQKANPDWRFVEDAGRG
                     YRRVVASPEPQRIVEAPAIKALIQQGFVVIGAGGGGIPVVCSEAGDYQSVDAVIDKDL
                     STALLAREIHADILVITTGVEKVCIHFGKPQQQALDRVDIATMTRYMQEGHFPPGSML
                     PKIIASLTFLEQGGKEVIITTPECLPAALRGETGTHIIKT"
     gene            9165..10547
                     /gene="yahJ"
                     /locus_tag="ACHKBL_002874"
     CDS             9165..10547
                     /gene="yahJ"
                     /locus_tag="ACHKBL_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFO5819722.1"
                     /translation="MKENNSRREFLSQSGKMVTAAALFGTSVPLAHAAVSGTIHCDAN
                     NTMKITDPHYYLDNVLLETGFDYENGVAVQTRTARQTVEIQNGKIVALRENNQHPDAT
                     LPHYDAGGKLMLPTTRDMHIHLDKTFYGGPWRSLNRPAGTTIQDMIKLEQKMLPELQP
                     YTQERAEKLIDLLQSKGTTIARSHCNIEPVSGLKNLQNLQAVLARRQAGFECEIVAFP
                     QHGLLLSKSEPLMREAMQAGAHYVGGLDPTSVDGAMEKSLDTMFQIALDYDKGVDIHL
                     HETTPAGVAAINYMVETVEKTPQLKGKLTISHAFALATLNEQQVDELANRMAAQQISI
                     ASTVQIGTLHMPLKQLHDKGVKVMTGTDSVIDHWSPYGLGDMLEKANLYAQLYIRPNE
                     QNLSRSLFLATGDILPLNEKGERVWPKAQDDASFVLVDASCSAEAVARISPRTATFHK
                     GQLVWGNVAG"
     gene            10843..11253
                     /locus_tag="ACHKBL_002875"
     CDS             10843..11253
                     /locus_tag="ACHKBL_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HFO5819723.1"
                     /translation="MTIKEKFSQKYPHASFCTFGDSAALADHLATLIATGVKTASCGS
                     LAGCIEDNAFPLIGEYKIVENSRGEPVCVIRVIGLHLLRFSDVTAELARKEGEGDLSL
                     EYWRNEHRRFFQAEGCYSPEMDVIFEEYALIDVV"
     gene            11507..12490
                     /locus_tag="ACHKBL_002876"
     CDS             11507..12490
                     /locus_tag="ACHKBL_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO5819724.1"
                     /translation="MNKRFVINMVSSLLLGAALISAPLQAAEKVVVNISKVDGMPWFN
                     RMGEGVVEAGKAFGVNASQVGPSSTDAPQQVKIIEDLIARKVNAITIVPNDANVLEPV
                     FKKARDAGIVVLTNESPGQPSANWDIEIIDNEKFAAEYVEHMAKRMGGKGGYVIYVGS
                     LTVPQHNLWADLLVKYQKEHYPDMHEVTRRMPVAESVDDSRRTTLDLMKTYPDLKAVV
                     SFGSNGPIGAGRAVKEKRAKNKVAVYGMMIPSQAASLIKSGDITEGITYDPASAGYAL
                     AAVASTLLKGEEIKPGLEMQNLGKADVDMDKRIIRFHKVLLVNKDNIDSLY"
     gene            12539..14023
                     /locus_tag="ACHKBL_002877"
     CDS             12539..14023
                     /locus_tag="ACHKBL_002877"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HFO5819725.1"
                     /translation="METFLSLRHINKTFHATRALRDVSLDFMSGEVHCLAGQNGCGKS
                     TLIKIMSGVYRPDEGAEITLGGKNWSKLTPAASVAQGIQVIYQDLSLFPNLSVWENIA
                     VNHYHHGLFVNRRRLREVAQAAMTSINVTLPLDTLVSELSIARCQLVAICRALAQDAR
                     LIVMDEPTASLTHQEVQGLLQVVHQLRERGICVVFVSHRLEEVMEVSDRISVLKDGEL
                     VGTFPAAEMTTKQLSFLMTGQEFEYQVRELWQGKSSTPVLEVRNLSRHGEYQNINLRV
                     EAGEVVSIVGLLGAGRTELCLSLFGMTRPDAGEILINGQPVTLHSNQDAIRHGIGYVS
                     EDRMSRGLVMAQSIEDNIISTVFHKVKDRFGFLSEAKVRDLVDRLIKALTIKVSDPHL
                     PVNTLSGGNAQRVSIAKWLAIGPRLLILDSPTVGVDIANKAGIYGIISDLAAHGIAVL
                     MICDEIEEAWYQSHRILVMQKGQITHSFLPDSSSQARIAEVVNG"
     gene            14016..14987
                     /locus_tag="ACHKBL_002878"
     CDS             14016..14987
                     /locus_tag="ACHKBL_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO5819726.1"
                     /translation="MAELKKRHEFWLALLIVVLFVGLAWRSDEFLTFGNLYDLANNYA
                     MLTILACGLFVVLISGGIDISFPAMTIIAQYGMVLLLQKMGGNFAVAFALAGGIGILL
                     GLINALLVNRLRVPSIIITISTLNIFYGLLLWLSKGVWLYDFPPWFEQGVMLFKYTDA
                     DGYDYGLGLPLIAMITVVLLTAFIMNFTSVGRKIYALGGNRESASRIGFSVLKLQLFV
                     YGYMGLMSGAAGVVQSWTVMTVAPDSLLGYELTVLAAVVLGGTSLLGGRGTLTGTLLG
                     VVLLAVMQNGLNLLGVSSYWQTLITGIIIVASISATAWSQHQNRSLL"
     gene            14984..15940
                     /locus_tag="ACHKBL_002879"
     CDS             14984..15940
                     /locus_tag="ACHKBL_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO5819727.1"
                     /translation="MKKSWRNNVEFYLIGLLVLTVAAFSITMPEIFWSISNFQSVASQ
                     MPVLGILALAMAVTMLCGGINLSIIATANACSLVMAWVATQYPPGIATVVATLLAGAG
                     AAVIIGLCNGVLIAGIRVSPILATLGMMTLLKGVNILVTGGSAIANYPSWVLWLNHAQ
                     WFGIPLPMWLFTAVALGLWILLEKTPLGKSIYLIGSNERATLYSGINTRRVLIWVYVI
                     SALLCAVAAFLMMSKLNSAKASYGESYLLVSILAAVLGGVNPDGGSGRIIGMVLALFL
                     LQIIESGFNILGISPYLTMALWGTLLLCFIQARGMLGLDRVV"
     gene            16027..17076
                     /gene="yahK"
                     /locus_tag="ACHKBL_002880"
     CDS             16027..17076
                     /gene="yahK"
                     /locus_tag="ACHKBL_002880"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase YahK"
                     /protein_id="HFO5819728.1"
                     /translation="MKIKAVGAYSAKQPLEPMDITRREPGPHDVKIEIAYCGVCHSDI
                     HQVRSEWAGTVYPCVPGHEIVGRVVAVGDQVEKYAPGDLVGVGCIVDSCKHCEECEDG
                     LENYCDHMTGTYNSPTPDEPGHTLGGYSQQIVVHERYVLRIRHPQEHLAAVAPLLCAG
                     ITTYSPLRHWQAGPGKKVGVVGIGGLGHMGIKLAHAMGAHVVAFTTSELKREAAKALG
                     ADEVVNSRNADEMAAHLKSFDFILNTVAAPHNLDDFTTLLKRDGTMTLVGAPATPHKS
                     PEVFNLIMKRRAIAGSMIGGIPETQEMLDFCAEHGIVADIEMIRADQINEAYERMLRG
                     DVKYRFVIDNRTLTD"
BASE COUNT         4245 a         4038 c         4640 g         4233 t
ORIGIN      
        1 tgctgaatgg attcagtctt aagaggcagg ttgttaaggg ataggcatag agtaatgacg
       61 tgtatgcata accaacatct ttactcatta tgtcattgaa tgttgacgct atgtgtttat
      121 gaggttgagg tattttcagt tgatctggat tgttaaattc atataatgcg cctttactca
      181 tgaatgggcg tcaatacgta gtgggaaatt ataaatatcg aaatagtcca actacttctt
      241 tattaccaaa aatgagtatc tgaattttaa tattgcattc ttgcgtgatt atctcctgag
      301 ttttgacttg tgattacctt tttaaggtat ttagcgtaac tgtttctgag cgagcatcag
      361 aggtaaagat aatcttcttg atagtgatgt gggatgttat acgtatggca tcgctaatgt
      421 ttatggttac cccttatgtg tgctcaggaa tccacaggta atcactcata ctgaacagcg
      481 ataaaagata aaaggtgtgt tcatgaattc atgtgatttt cgtgtttttc tgcaagagtt
      541 cggtacaacg gttcatttgt cattgcctgg tagcgtatcc gagaaagaac gactgctact
      601 caagctgctg atgcagggaa tgtctgtaac agaaatatca cagtacagaa atcgtagtgc
      661 aaagaccatt tcacatcaaa agaaacagct ctttgagaaa ctggggattc agagcgatat
      721 tactttctgg cgggatattt tctttcagta caatccggag atcatatccg ccacgggtaa
      781 taatagtcac aaatatatta atgataatca ctatcaccat atcgtcacgc ctgaagccat
      841 cagtctggcg ttggaaaacc atgaatttaa accgtggatc caaccggttt tctgcgcgca
      901 gactggggta ctgacgggct gtgaggtgct tgtccgctgg gaacatccac aaacgggaat
      961 tatcccaccg gatcagttta ttcctctggc ggagtcatcc ggtcttattg tcataatgac
     1021 ccgccagttg atgaaacaga ctgcggatat tctgatgccg gtaaaacatt tgctgccgga
     1081 caatttccat attggcatca acgtctcggc gggttgtttt ttggctgcgg gatttgaaaa
     1141 agagtgtctg aacctggtta agaaattagg taacgataaa atcaaactgg ttctcgagct
     1201 aacggaacgt aaccctattc cggtaacgcc agaagccaga gcgatatttg acagccttca
     1261 tcagcacaac attacctttg cgctggatga ctttggtacg ggttatgcga cctatcgtta
     1321 cttgcaggcg ttcccggtcg attttattaa gatcgataag tcatttgtgc aaatggcgag
     1381 cgtggacgaa atatccggtc atattgtgga caatattgtc gaactggcgc gtaagcctgg
     1441 tctgagtatc gtggcggaag gggtagaaac ccaggagcag gcggatttaa tgatcggcaa
     1501 aggcgttcac tttttgcagg gctatttgta ctctccgcca gtaccgggta ataaatttat
     1561 ctctgaatgg gtaatgaaag caggtggttg atgtaaaccg ctattcacag cgcatcggga
     1621 ggttggcagc gattaattct cccgatgcag taaatcctga tagatccccg ttaatatgcc
     1681 atttggcgaa aattgttttt taatccactg cgtcacctga cccgtagcag aatgctgagt
     1741 tgcgagtaac atgcgtgaat cctggcgcgg attatggatt tgtcgggtaa ccagcaggga
     1801 ttgcgccatc gcctcacgga ccatgtaatc cggcaaaaaa ccaattcctt cacctaaaat
     1861 ctggcactga catttggtgt tgaaatctgg caccagaatc gactcctgcc catgcagcaa
     1921 ccagcccact tttttattaa tcgtatgcgc ggtgtcctcc accatgatat tggggtaaag
     1981 acgcagctgg ctttcggcga tcggctccgg cacaaaggct aatgggtgat ccggggcgat
     2041 agcaaaagcc cagcgaattg cgccaatctc cgtgtaatca atgccgccgc cgtccagcag
     2101 cgtatcgggt gcgccgatag caatattcgc ctgattatta ataatcgcat cccagacgcc
     2161 gttatacact tcggtcgtca cggtaatctg gcaggttgga aactgctttt tcagtacctg
     2221 taataagcgt gcggtgtgtt tgggcgtata aagcagttgg ttaatacaaa tgcgcacccg
     2281 tgcttctata ccctgcgaaa tagtatcgat gctgcgtttg atggcgtgaa aatcattcag
     2341 caggtcggta gctttgcgga aaaaataacg cccggactcc gttaactcaa tgcttcgggt
     2401 actgcgagtg aacagcacca catccagccc tgtttccatt cgcttgatgg tgtagctaat
     2461 ggcggaagtg gttaaaccca actcttctgc ggctttactg aaactgccaa aacgcgcagc
     2521 ggtagtaaat gccaacagat tctcttcggt aaaaattgag ttcattaaac cattcccgcc
     2581 attatcgaat atgaatttct ccagctatga ctgtagaggt atcggttaaa gatagataat
     2641 catttttgaa taacttttaa tacccgtcgc gtttagtcgt cgcttcgctt aatttttgtc
     2701 cgccatattc tgaaattatc cccacgcagt tacccgccag aaaacccagt aatgtcaccg
     2761 tccagttcat cccgctggca aaaaatagcg tcatgccgag aaagcctccg ggaataaaag
     2821 acagtaaacc gaaacgtcct tgccagacca ccaccgccga gaagggtaca gtcataatca
     2881 cgctcgccca gaacccggaa atcccactta cagaggccag ccagccggca gccagcgcgg
     2941 cgaaaaaggc ccacaccatc ccgaaataat taaccgccag acttctgata aaccccgatt
     3001 taccgccgcc tgcggcataa aaactgcaaa aggcgacgaa gccaatggtt cctaataatt
     3061 cccatccctg agagagcccg acatgtgaag acactaactg ccaaagccct gcacaaatac
     3121 cgacggtgac acccgttgcc gtgagtccgt tcatcttgtt cccctctggt tagacatgga
     3181 cgaaagctac tcttacatgt gcagactata gagaaataaa acctgattga gtgaaagggt
     3241 atcgggtcaa agaaacaaat attgcactac cgcacactgc gaaaagattg ttgaacatca
     3301 ttcaacaaaa aggcgaaata cggcgaatta aatttaagcg ggttctttta tcagattatc
     3361 gttgctattc tcaattcatc gaaaacagcc acaaaaacca ggggaatgtg aatgactata
     3421 aaaaatctac ctgccgacta tttattggct gcgcaacagg gtgatatcga taaagtaaaa
     3481 acctgccttg cgctgggtgt cgatattaat acctgcgatc gtcaggggaa aacggcaatt
     3541 acgctggcaa gtttatatca gcaatatgct tgcgttcagg cattaattga tgccggagcg
     3601 gatattaata agcaagatca tacctgttta aatccttttt taattagctg tctgaacgat
     3661 gatttaacgc tactacgaat tattttaccg gctaaacccg atcttaattg cgtcacccgt
     3721 tttggcggtg tcggcctgac gcctgcctgt gaaaaaggcc atttaaatat tgtaaaagag
     3781 cttttggcgc atacggagat taacgttaac cagaccaacc atgttggctg gacgccgcta
     3841 ctggaagcga ttgtgcttaa tgatggcggg attaaacagc aggcgattgt ccagttattg
     3901 ctggaacacg gtgccagccc gcacatgacg gataaatatg gcaaaacgcc gctggaactg
     3961 gcgcgggaac gaggctttga agagattgcg cagttactga ttgccgcagg tgcataaacc
     4021 gggaggcttg ctatcaacac accagaaaga cggtgtgtgt gggcgctaac tgcggatgct
     4081 gattttctgg cgcagcgggg gcaaggacag gttgaacagg tctttgccag agcggtaaat
     4141 atcgcacttc cggcccgcca gcagttgctg acgctgcttt gtgaagagta cgacaatgcg
     4201 ccaaacagtt gtcggttggc actcactcac tttgatggtc tgttccggca tggtgataag
     4261 gttcagtttg acgatcaagg cattacggtt ggtcaacatc ttcatataga gatgagtcgt
     4321 tgtcggcgtt ggctgtcccc aaccttgcaa atggccgctg tgaattttca ccttatcgcc
     4381 tggcaacagt ggcatgacat tattcatcag cacctggggg aaaatgaaac cctgtttaat
     4441 tattgcggcg ataatccgtt ttatcaggcg ttaaataaag aattacatat taaacgacgg
     4501 gcagttattc aggccgtaaa cgataaacaa aatatcgcct cagcagtcgc cagtatgatg
     4561 ggcttaggga ttggccttac gccatcagcc gacgattatt taacaggtct ggcgcttatt
     4621 ttatttattt ccggacatcc ggcggaaaaa tacaaagagg aattttatct cggtctgcaa
     4681 tgcggcagaa ataataccac gttattaagt gccataacgc tggaagccgc attacaacaa
     4741 cgctgccggg aaaatattca tcgttttatt cataacatta tttatgacat ccctgggaac
     4801 gcaactcagg caatagaaaa aattaaacat attggctcca gttccggctg cgacatgctg
     4861 tatggcatgg ccgatggttg tgcgctgagc caaacctacg gagggaatta tgtcagttaa
     4921 aatagtcatt aaaccgaata cctattttga ttctgtctcg ctgatgtcta tctccacgcg
     4981 tgcaaataaa ctcgacggcg tcgagcaggc atttgtggcg atggcgaccg aaatgaacaa
     5041 aggcgtgctg aggaatttag gactgctgac gccggaactg gagcaggcga aaaacggtga
     5101 cctgatgatt gtcatcaatg gtaaatcggg tgcggacaac gagcagttgc tggtggagat
     5161 tgaagaactg ttcaacagca aagcgcaaag tggctcgcac gaggcgcgtt acgccactat
     5221 tgccagcgcc aaaaagcata tcccggaaag taacctggcg gtgatttcgg tcaacggtct
     5281 gtttgccgct cgcgaagcgc gtcaggcgct gcaaaacgat cttaacgtga tgctgttttc
     5341 cgataacgtc tcagttgaag atgaactggc gctcaaacag ttggcccacg aaaaagggct
     5401 gctgatgatg gggccagact gcggtacggc gattatcaac ggcgcggcgc tctgctttgg
     5461 taacgccgtg cgtcgcggca acatcggcat tgttggcgca tccggcaccg gtagccagga
     5521 actgagcgtc cgcattcatg aatttggcgg cggcgtttcg caactgatcg gcaccggcgg
     5581 gcgggacctg agcgagaaaa tcggcggcct gatgatgctc gacgctatcg ggatgctgga
     5641 aaacgatccg caaactgaaa tcattgtgct tatctccaaa ccgccagcgc ctgcggtggc
     5701 ccgcaaagtg ctggaacgcg cacgcgcctg ccgtaagcca gtggtagtct gcttcctcgg
     5761 tcgtggcgaa acgccagtgg atgagcaggg gctacagttt gctcgcggca ccaaagaagc
     5821 ggcactaaaa gcggtgatgc tctccggtat gaaacaagaa catctcgact tgcataccct
     5881 gaaccagccg ttgattgcgg atgtgcgtgc gcgtatgcaa ccgcagcaga aatacattcg
     5941 tggcctgttc tgcggcggca cgctgtgtga cgaaaccatg tttgcggtga tggaaaaaca
     6001 tggcgatgtc tacagcaaca ttcagcccga tcctgaattc cgcctgcaag atatcaaccg
     6061 cagcgtcaaa cataccttcc tcgactttgg cgatgacgat tttaccaacg gcaagccgca
     6121 tccaatgatt gacccgacca accgcatcag ccggttgatc gaagaagcgc gcgatccaga
     6181 agtggcggtg atcgtgatgg attttgtgct cgggtttgga tcgcatgaag atccggtcgg
     6241 ttccaccatc gaggcgatca aagaggcgaa agcgatcgcc gctgccgaag ggcgcgagtt
     6301 gatcattctc gcctatgtgc tgggtaccga tctcgatacg ccatcgttag aacaacaaag
     6361 ccagatgttg cttgatgccg gagtgattct ggccagcagc agcaccaata ccggattgct
     6421 ggcgcgtgaa tttatctgca aaggggagga agcctgatga gcccgtcact gtttagccaa
     6481 ccgttgaacg ttattaacgt cggtatcgcc atgtttagcg atgatctgaa aaagcagcat
     6541 gtagaagtga cccaactcga ctggacgccg ccggggcagg gcaatatgca ggtggtgcag
     6601 gcgctggaca acattgccga ttccccgctg gcggacaaaa tcgccgccgc taaccagcag
     6661 gcgctggagc gcattatcca gtcgcatccg gtgctgattg gttttgatca ggcgattaac
     6721 gtggtgccgg gtatgacgcc gaaaaccatt cttcacgccg ggccgccgat cacctgggaa
     6781 aaaatgtgtg gcgcgatgaa aggcgcggtc accggagcgc tggtgttcga agggctggcg
     6841 aaagatctcg atgaggcggc tgaactggct gcctccgggg agatcacctt ctcgccgtgc
     6901 cacgagcacg actgcgtggg ttcgatggcg ggtgttacct cggcctcgat gtttatgcac
     6961 atcgtaaaaa acaaaaccta cggcaacatc gcttacacca atatgagcga gcagatggcg
     7021 aagattttgc gtatgggcgc taacgaccag agcgtgattg accgcctcaa ctggatgcgc
     7081 gatgtgcagg ggcccatact gcgcgacgcg atgaaaatta tcggcgaaat cgatctgcgc
     7141 ttaatgctgg cgcaagcgct gcatatgggc gatgagtgcc ataaccgcaa taacgccggg
     7201 acgacgctgc tgattcaggc gctgacgcca gggattattc aggcgggcta ttccgtcgaa
     7261 caacagcggg aagtgtttga gtttgtcgcc agcagcgact acttctccgg cccgacgtgg
     7321 atggcgatgt gtaaagcggc gatggatgcg gcgcacggca ttgaatacag cactgtggtc
     7381 accaccatgg cgcgtaacgg cgtcgagttc ggcctgcggg tcagcggcct gccggggcaa
     7441 tggtttaccg gcccggcgca gcaggtgatc ggcccgatgt ttgccggtta taagccggaa
     7501 gattcggggc tggatatcgg cgacagcgcc atcactgaaa cctacggcat tggcggattt
     7561 gccatggcga ccgcgcctgc aatcgttgcg ctggtgggcg gcacggtgga agaggctatc
     7621 gatttctccc gccagatgcg tgaaatcact ctcggtgaaa accccaacgt caccattccg
     7681 ctgctcggtt ttatgggggt gccgacggca atcgacatca cccgcgtggg cagtagcggc
     7741 attctgccgg tgatcaacac cgccatcgcc cataaagatg cgggcgtcgg catgattggc
     7801 gcgggcattg tgcatccacc attcgcctgc ttcgagaaag ccattcttgg ctggtgcgaa
     7861 cgttacggcg tctgagttac atgtttatgc cggatgcgac atgaatgtct tatccggcct
     7921 acgaatggcg cggaaatctg taggcctgat aagcgtggcg cttcaggcat tggccaccac
     7981 agccggatgc gacgtgaacg ccttatccgg cctacgaatg gcacggaaat ctgtaggcct
     8041 gataagcgta gcgcatcagg cattggtcac cacagccgga tgcggcgtga acgccttatc
     8101 cggcctacga gtggcgcaag aatctgtagg cctgataagc gtagcgcatc aggcagtctg
     8161 gcgttggtca taaccccatc accctctgta gcggacataa aaatatgaaa gaacttgtgg
     8221 tcgttgccat tggtggcaac agcattatca aagataacgc cagccagtcg attgagcatc
     8281 aggcggaggc ggtgaaagcc gtcgccgata cggtgctgga aatgctggct tccgattacg
     8341 acattgtgct gacccacggt aacgggccgc aggtcgggct ggatttacgc cgcgcggaga
     8401 ttgctcacga gcgcgaaggg ctgcccttaa cgccgctggc aaactgtgtg gcggatacgc
     8461 aaggtggcat tggctatctg atccagcagg cgctgaacaa ccggctggcc cgccacggcg
     8521 agaaaaaagc cgtcaccgtg gtgactcagg tggaagtgga taaaaacgat ccgggatttg
     8581 cccatcccac caagcctatc ggcgtattct tcagtgaaag ccagcgtgac gaattacaaa
     8641 aggcaaaccc tgactggcgt tttgttgaag atgccggacg gggctatcgc cgcgtggtcg
     8701 cctcgccgga accgcaacgt attgtagaag cacctgccat taaggccctg atccaacaag
     8761 gctttgtggt gattggcgcg ggcggcggcg gaattcctgt ggtgtgcagt gaagcgggag
     8821 attaccaaag cgtggacgcg gttatcgaca aagatctctc taccgcgcta ttggcccgtg
     8881 aaattcacgc cgacattctg gtaattacca ccggcgtcga aaaagtgtgt attcactttg
     8941 gcaaaccgca gcagcaggcg ctggatcggg tggatattgc caccatgacc cgctatatgc
     9001 aggaagggca tttcccaccc ggcagcatgt tgccaaaaat catcgccagc ctgacgttcc
     9061 tggagcaggg cggtaaagaa gtgattatca ccacgccgga atgcctgcct gcggcgctgc
     9121 gtggtgaaac gggcacccat attattaaaa cgtaaggacg taagatgaaa gaaaacaata
     9181 gccgccgtga gtttctgagc cagagcggta agatggtcac cgccgccgcg ctgtttggta
     9241 cctctgtgcc gctcgcccat gcggcagtct ctggcacaat ccattgcgac gcgaataaca
     9301 ccatgaaaat cactgacccg cactactatc tcgacaacgt gttgctggaa accggttttg
     9361 actacgaaaa tggcgtggcg gtgcagaccc gcacggcgcg ccagactgtg gaaattcaga
     9421 acggcaaaat tgttgccctg cgcgagaaca atcagcatcc ggacgccacg ctgccgcact
     9481 atgacgctgg cggtaagctg atgctgccca ccacccgcga catgcatatt catctcgaca
     9541 aaaccttcta tggcgggccg tggcgctcgc tcaatcgtcc ggcaggcacc accatccagg
     9601 acatgatcaa actcgagcag aaaatgctgc cggagttgca accgtacact caggaaaggg
     9661 cggaaaaact gattgattta ttgcagtcga aaggcactac cattgcccgc agccactgca
     9721 atatcgaacc ggtttccggc ctgaaaaatc tgcaaaattt gcaggcggtt ctggcgcgac
     9781 gtcaggcggg cttcgagtgt gaaattgtcg ccttcccgca gcacggtttg ctgctgtcga
     9841 aatcagaacc cttaatgcgc gaagcgatgc aggcgggggc gcattacgtc ggcgggctgg
     9901 acccgaccag tgttgatggc gcgatggaaa aatccctcga caccatgttc cagattgcgc
     9961 tggactacga caaaggcgtc gatattcacc tgcacgaaac cactccggca ggcgtggcag
    10021 ccatcaatta tatggttgaa acggtagaga aaacgccaca gctgaagggc aagctgacca
    10081 tcagccacgc cttcgcgttg gcaacgctca acgaacaaca ggtagatgaa ctggcgaacc
    10141 ggatggcggc gcagcaaatt tctatcgcct cgacggtgca gatcggcacg ctacatatgc
    10201 cgcttaaaca actgcacgac aaaggcgtaa aagtcatgac cggaactgac agcgttattg
    10261 accactggtc gccttacggc ctgggcgaca tgctggaaaa agccaatctc tacgcgcagc
    10321 tctatattcg tcctaacgaa cagaacctct cccgttcgct gtttttagct actggcgata
    10381 tattgccgct caacgaaaaa ggcgagcgtg tgtggcctaa agctcaggat gacgccagct
    10441 ttgtgctggt ggacgcctcc tgttccgccg aggcggtggc gcgtatctcg ccgagaaccg
    10501 caacgttcca taaagggcaa ctggtgtggg ggaatgtggc aggttgatgc gcatttgata
    10561 tgtctgcaac ggtgaacagt aagagattta agccccaggg agtggggcgg gaatggtttg
    10621 ttaatgaatg atcctgaact ttataatctc ccggcataat ttccagggga ttatttgaga
    10681 gaaatacaca aaagtgagtt ttaacaaagg tatttatcaa gccataaatg tgacctgctt
    10741 cggataaaaa agattaaaca taggaaaacc aatgatactc ttgctgatgg ttaccaacag
    10801 taattaagat gtgatttttt ccgtaatcga aaaaatattg ccatgaccat aaaagaaaaa
    10861 ttctcacaaa aatatcccca tgcttccttt tgtacttttg gcgattcagc agcgttggcc
    10921 gatcacctcg caacgttgat tgcgactggt gttaaaacag cttcctgtgg ttcgctggcg
    10981 ggatgtattg aagacaacgc atttccgctg attggtgagt ataagattgt agaaaatagc
    11041 cgtggtgagc ctgtctgtgt tatcagagtg attggcctgc atttactgcg cttttctgat
    11101 gtcaccgcag agcttgcccg taaggaaggt gaaggtgacc tcagtcttga atattggcgt
    11161 aatgaacacc gtcgtttttt ccaggcagaa ggatgctatt caccagaaat ggacgttatc
    11221 tttgaagaat atgcccttat tgacgttgtg tagataacta tttttctacg cgaaataagc
    11281 tcacaaacat aaagtatcgc taattttgtt tattttgctg ttttttacgt gatgattgtc
    11341 acacttattg ttgctgcccg ttttgtccat atttataacc aaatttttaa tagctgcctg
    11401 aaatagggct gtttattctg cgtaaaaata ttcgaaaatt cttattctct ccagcgcgct
    11461 aatttgggcg aacacttcct gactaccctg caatgaggct gaaatgatga ataaacgttt
    11521 tgttatcaat atggtgtctt cgctgttatt aggcgctgcg ctaatttctg cgccgctcca
    11581 ggcagcggaa aaagtcgtgg tgaatatctc taaagtcgat ggtatgccgt ggtttaatcg
    11641 catgggcgaa ggtgtggttg aggcgggtaa agcctttggt gttaatgcgt cacaggtggg
    11701 gccttcaagt actgatgctc cgcaacaggt taaaattatt gaggatttaa tcgcccgtaa
    11761 ggttaatgca attactatcg tgccaaacga tgccaacgtg ctggaaccgg tattcaaaaa
    11821 agcgcgagat gccggaattg tggttctgac caacgaatct cccggtcagc caagtgctaa
    11881 ctgggatatc gaaatcattg ataacgaaaa atttgccgct gaatatgttg agcatatggc
    11941 gaaacgcatg gggggcaagg gcggctatgt gatttacgtg ggtagtttga ccgtgccgca
    12001 gcataactta tgggctgact tgctggtgaa ataccagaaa gagcattacc cggatatgca
    12061 tgaagttacc cgccgcatgc cggttgccga gagtgtggat gactcacgtc gtaccacact
    12121 ggacctgatg aaaacctatc ctgacttgaa agcggttgtc tcctttggtt caaacggtcc
    12181 gattggcgcg ggacgtgcag tgaaagagaa acgggcgaaa aataaagtcg cagtctatgg
    12241 catgatgatc ccttcacagg cggcatcact gattaaaagt ggtgatatca ccgaagggat
    12301 tacttacgat ccggcctctg caggttacgc actcgcggcc gtcgccagca cgttgctgaa
    12361 gggtgaagag atcaaacctg gattagaaat gcagaatctc ggtaaggccg acgtagatat
    12421 ggataagcga attatccgtt tccataaggt attactggtc aacaaagaca atatcgactc
    12481 actttattaa tccatttctt cggggcgaaa gccccgattc ttattgccag gggatgctat
    12541 ggaaaccttc ctttcccttc gtcatatcaa caaaacgttt catgccacgc gagcgctgcg
    12601 cgatgtttct ctcgacttta tgtcgggtga ggtgcactgc cttgccgggc aaaacggctg
    12661 tggtaaatcg acgttgatta aaattatgtc cggtgtttat cgcccggatg agggggcgga
    12721 aattacgctt ggtgggaaaa actggtcaaa gctgacaccc gccgcttcgg tggcgcaggg
    12781 gattcaggtg atttatcagg acctctcttt atttcctaac ctgagtgtct gggagaatat
    12841 cgccgtgaat cactatcacc acggtctgtt tgttaaccgc cgtcgtctgc gtgaggtggc
    12901 gcaggcggca atgaccagta ttaacgtcac attgccactg gatacgctcg tttctgagct
    12961 ttcaattgcc cgctgtcagc tggtggcaat ttgccgtgca ctggcgcagg acgcacggtt
    13021 gatcgtgatg gatgaaccaa ccgcttcgct gacccatcag gaagtgcaag ggttattaca
    13081 ggtggtgcat caattgcggg aacgcgggat ctgcgtggtc ttcgtcagtc atcgtctgga
    13141 agaagtgatg gaagtttccg accgtatttc agtgctgaaa gatggtgagc tggtcgggac
    13201 ctttccggcc gcagagatga ccacaaaaca actcagtttt ctgatgaccg gccaggagtt
    13261 cgaatatcag gtacgagagt tgtggcaggg aaaatccagc accccggtgc tggaggtgcg
    13321 taatttaagt cgtcatgggg aatatcaaaa tattaacttg cgggtggaag cgggcgaagt
    13381 ggtgtcgatt gttggcctgc ttggtgcggg gcgcacggaa ttatgtctga gtctgtttgg
    13441 tatgacccga ccggatgctg gtgagatcct catcaacggt cagccggtta cactgcatag
    13501 taaccaggac gccatccgcc acggtattgg ttatgtttcg gaagatcgca tgtcgcgcgg
    13561 tttggtgatg gcgcaatcta ttgaagacaa catcattagc actgtttttc acaaggtaaa
    13621 agatcgcttc ggctttctga gtgaagctaa agtgcgtgac ctggtggaca ggctaatcaa
    13681 ggcattgacc atcaaagtct ctgacccaca tctcccggtg aatacgcttt ccgggggtaa
    13741 cgcgcagcga gtatcgatcg ccaaatggct ggcgattggg ccacgactgt tgattctcga
    13801 tagcccgact gtcggtgttg atatcgccaa caaagcagga atatacggca ttatcagcga
    13861 tctggcggct catggcattg cggtattgat gatctgcgat gaaattgaag aagcgtggta
    13921 tcaaagccac cggattttgg taatgcaaaa aggtcagatc acccatagct tcctgcctga
    13981 cagcagttct caggccagga ttgcggaggt ggtaaatggc tgaactgaaa aaacgccatg
    14041 aattctggct ggcattactg attgtggtgc tgtttgttgg ccttgcctgg cgcagcgacg
    14101 agtttctgac attcggtaat ttgtacgatc tcgccaataa ctatgccatg ttgactattc
    14161 tcgcctgtgg tttgtttgtg gtgttaattt ccggtgggat tgatatttcg tttccggcaa
    14221 tgaccatcat tgcgcaatac ggcatggtgc tgttgctgca aaaaatgggt ggcaactttg
    14281 ctgtcgcgtt tgccctggcg ggcggcatcg gcattttact tggcttaata aacgccttac
    14341 tggttaatcg cctacgggtg ccgtctatca tcatcactat ctcgacgctg aatatttttt
    14401 atggcctgct gttatggttg agtaaaggtg tgtggctgta cgactttccg ccgtggtttg
    14461 agcagggggt aatgttgttc aagtacaccg atgctgatgg ctatgactat ggccttggtc
    14521 tgccgctgat cgccatgatt acggtggtgc tgctgacagc gtttatcatg aatttcacca
    14581 gtgtagggcg caaaatttat gcccttggcg ggaaccgcga atcagccagt cgcatcggct
    14641 ttagtgtgct gaaactgcaa cttttcgtct atggctatat gggattgatg tctggtgctg
    14701 cgggtgtagt gcagtcgtgg acggtgatga ctgtcgcccc cgattctctt ctgggttatg
    14761 agctgacagt actggctgcg gtggtgcttg gtggcactag tttgctcggc gggcgcggca
    14821 cgttaaccgg tactttgctc ggcgtggtgt tgttggcagt aatgcaaaac gggctaaatt
    14881 tattgggagt ctcgtcttac tggcaaacat tgatcacagg catcatcatc gttgccagca
    14941 ttagcgccac ggcgtggagt cagcatcaga accggagtct gctatgaaaa aatcgtggcg
    15001 taataacgtt gagttttact tgatcggcct gctggtgcta actgttgccg cgttcagcat
    15061 tactatgccg gagattttct ggtcgataag taatttccag tccgtcgctt cgcaaatgcc
    15121 cgtgctgggc attctggcgt tggctatggc ggtgaccatg ctttgcggtg gcattaactt
    15181 gtcaattata gccactgcta acgcctgttc actggtgatg gcgtgggtgg cgactcagta
    15241 tccaccgggt attgctacgg ttgtcgctac gctgctggcg ggcgctggcg cggcggtgat
    15301 aatcggttta tgtaacggcg tattgattgc tggtattcga gtgtcgccaa ttcttgcaac
    15361 gcttgggatg atgacgttgc tcaaaggggt aaatattctg gtgacaggcg gtagcgccat
    15421 tgccaattat ccctcatggg tgctgtggtt gaatcatgca cagtggttcg gtattccgct
    15481 accgatgtgg ttgtttaccg cagtggcgct ggggctgtgg atattgctgg agaaaacgcc
    15541 gctcggtaaa tccatttatc tgattggttc taacgaacga gcaacacttt actccggtat
    15601 taacactcgt cgggtgctga tatgggtgta tgtcatttcc gccttgcttt gtgcggtagc
    15661 ggcatttttg atgatgtcga aactcaactc ggctaaggcc tcttatggcg aatcttactt
    15721 gctagtgtca atcctcgcgg cagtactcgg cggggttaat ccggacggcg gcagcgggcg
    15781 gataattggt atggtgctgg cgctgtttct tctgcaaatt attgaaagcg gcttcaatat
    15841 tctcggtata agtccctacc tgacgatggc cttatgggga acgttgctgc tctgtttcat
    15901 tcaggctcgc ggcatgttgg ggctggatcg ggtggtttaa atattggcta tattcaatgg
    15961 acgcgttttg ccgcgatgac atatcaggca tcgccaaata cacatagcta atcaggagta
    16021 aacacaatga agatcaaagc tgttggtgca tattcagcta aacaaccact tgaaccgatg
    16081 gatatcaccc ggcgtgaacc gggaccgcat gatgtcaaaa ttgaaatcgc ttactgtggc
    16141 gtctgccatt ccgatatcca ccaggtccgt tccgagtggg cggggacggt ttatccctgc
    16201 gtgccgggcc atgaaattgt aggacgcgtg gtagctgttg gtgatcaggt agaaaaatat
    16261 gcgccgggcg atctggttgg tgtcggctgc attgtcgaca gttgtaaaca ttgcgaagag
    16321 tgtgaagacg ggctggaaaa ctactgtgat cacatgaccg gcacctataa ctcgccgacg
    16381 ccggacgaac cgggccatac tctgggcggc tactcacaac agatcgtcgt tcatgagcga
    16441 tatgttctgc gtattcgtca cccgcaagag cacctggcgg cggtggctcc tttgttgtgt
    16501 gcagggatca ccacatactc gccgctacgt cactggcagg ccgggccggg taaaaaagtg
    16561 ggcgtggtcg gcatcggcgg tctgggacat atggggatca agctggccca cgcgatgggg
    16621 gcgcatgtgg tggcatttac cacttctgag ttaaaacgcg aagcggcaaa agccctgggg
    16681 gccgatgaag ttgttaactc acgcaatgcc gatgagatgg cggctcatct gaagagtttc
    16741 gatttcattt tgaatacagt agctgcgcca cataatctcg acgattttac caccttgctg
    16801 aagcgcgatg gcaccatgac gttggttggt gcgcctgcga caccgcataa atcgccggaa
    16861 gttttcaacc tgatcatgaa acgccgcgcg atagccggct ctatgattgg cggcattcca
    16921 gaaacacagg agatgctcga tttttgcgcc gaacatggca tcgtggctga tatagagatg
    16981 attcgggccg atcaaattaa tgaagcctat gagcgaatgc tgcgtggtga tgtgaaatat
    17041 cgttttgtta tcgataatcg cacactaaca gactgaaaaa attaataaac atcctgtggt
    17101 ttaacatatt aacttcgctc cacttaactt tttagttaag gagagcgaaa taatat
//
LOCUS       DAZFLD010000106        17010 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.107, whole genome shotgun sequence.
ACCESSION   DAZFLD010000106 DAZFLD010000000
VERSION     DAZFLD010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17010
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.107"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..234
                     /locus_tag="ACHKBL_002881"
     CDS             <1..234
                     /locus_tag="ACHKBL_002881"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5819729.1"
                     /translation="SFIMISVFTTSPFRQDRPKFHAYTICVLAIDPFLTLRVVFPAYR
                     NTFVVRKVCKGKRLPCDFAGAEVRVWSEMEWQQ"
     gene            222..1247
                     /gene="purR"
                     /locus_tag="ACHKBL_002882"
     CDS             222..1247
                     /gene="purR"
                     /locus_tag="ACHKBL_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HFO5819730.1"
                     /translation="MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELH
                     YSPSAVARSLKVNHTKSIGLLATSSEAAYFAEIIEAVEKNCFQKGYTLILGNAWNNLE
                     KQRAYLSMMAQKRVDGLLVMCSEYPEPLLAMLEEYRHIPMVVMDWGEAKADFTDAVID
                     NAFEGGYMAGRYLIERGHREIGVIPGPLERNTGAGRLAGFMKAMEEAMIKVPESWIVQ
                     GDFEPESGYRAMQQILSQPHRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDN
                     VRNARYFTPALTTIHQPKDSLGETAFNMLLDRIVNKREEPQSIEVHPRLIERRSVADG
                     PFRDYRR"
     gene            complement(1244..2176)
                     /gene="punR"
                     /locus_tag="ACHKBL_002883"
     CDS             complement(1244..2176)
                     /gene="punR"
                     /locus_tag="ACHKBL_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator PunR"
                     /protein_id="HFO5819731.1"
                     /translation="MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWL
                     AVPLFERRHRDVELTAAGAWFLKEGRSVVKKMQITRQQCQQIANGWRGQLAIAVDNIV
                     RPERTRQMIVDFYRHFDDVELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYAF
                     RDMGMLSWSCVVASHHPLALMDGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKR
                     LVVPDWESSATCISAGLCIGMVPTHFAKPWLNEGKWVALELENPFPDSACCLTWQQND
                     MSPALTWLLEYLGDSETLNKEWLREPEETPATGD"
     gene            2289..3500
                     /gene="punC"
                     /locus_tag="ACHKBL_002884"
     CDS             2289..3500
                     /gene="punC"
                     /locus_tag="ACHKBL_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1990961 - xenobiotic detoxification by
                     transmembrane export across the plasma membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside transporter PunC"
                     /protein_id="HFO5819732.1"
                     /translation="MQPGKRFLVWLAGLSVLGFLATDMYLPAFAAIQADLQTPASAVS
                     ASLSLFLAGFAAAQLLWGPLSDRYGRKPVLLIGLTIFALGSLGMLWVENAATLLVLRF
                     VQAVGVCAAAVIWQALVTDYYPSQKVNRIFATIMPLVGLSPALAPLLGSWLLVHFSWQ
                     AIFATLFAITVVLILPIFWLKPTTKARNNSQDGLTFTDLLRSKTYRGNVLIYAACSAS
                     FFAWLTGSPFILSEMGYSPAVIGLSYVPQTIAFLIGGYGCRAALQKWQGKQLLPWLLV
                     LFAVSVIATWAAGFISHVSLVEILIPFCVMAIANGAIYPIVVAQALRPFPHATGRAAA
                     LQNTLQLGLCFLASLVVSWLISISTPLLTTTSVMLSTVVLVALGYMMQRCEEVDCQNH
                     GNAEVAHSESH"
     gene            3791..4939
                     /gene="cfa"
                     /locus_tag="ACHKBL_002885"
     CDS             3791..4939
                     /gene="cfa"
                     /locus_tag="ACHKBL_002885"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HFO5819733.1"
                     /translation="MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNP
                     DFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIAGAR
                     LFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMIC
                     EKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSVVGVTISAEQQKMAQERCEGLDVTIL
                     LQDYRDLNDQFDRIVSVGMFEHVGPKNYDTYFAVVDRNLKPEGIFLLHTIGSKKTDLN
                     VDPWINKYIFPNGCLPSVRQIAQSSEPHFVMEDWHNFGADYDTTLMAWYERFLAAWPE
                     IADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGLRVAR"
     gene            complement(4979..5620)
                     /gene="ribE"
                     /locus_tag="ACHKBL_002886"
     CDS             complement(4979..5620)
                     /gene="ribE"
                     /locus_tag="ACHKBL_002886"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004746 - riboflavin synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HFO5819734.1"
                     /translation="MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAH
                     NGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGH
                     IMTTAEVAKILTSENNRQIWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVH
                     LIPETLERTTLGKKKLGARVNIEIDPQTQAVVDTVERVLAARENAMNQPGTEA"
     gene            5835..7208
                     /gene="mdtK"
                     /locus_tag="ACHKBL_002887"
     CDS             5835..7208
                     /gene="mdtK"
                     /locus_tag="ACHKBL_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MdtK"
                     /protein_id="HFO5819735.1"
                     /translation="MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDM
                     AAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLI
                     MLVLWNAGYIIRSMENIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
                     MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSM
                     RDIRNEKGTAKPDPAVMKRLIQLGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQI
                     ALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLMVGVCMATLTAIFTV
                     SLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITF
                     TAYWVLGLPSGYILALTDLVVEPMGPAGFWIGFIIGLTSAAIMMMLRMRFLQRLPSAI
                     ILQRAAR"
     gene            complement(7249..8505)
                     /locus_tag="ACHKBL_002888"
     CDS             complement(7249..8505)
                     /locus_tag="ACHKBL_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HFO5819736.1"
                     /translation="MGSDAKNLMSDGNVQIVKTGEVIGATQLTEGELIVEAGGRAENT
                     VVTGAGWLKVATGGIAKCTQYGNNGTLSVSDGAIATDIVQSEGGAISLSTLATVNGRH
                     PEGEFSVDKGYACGLLLENGGNLRVLEGHRAEKIILDQEGGLLVNGTTSAVVVDEGGE
                     LLVYPGGEASNCEINQGGVFMLAGKASDTLLAGGTMNNLGGEDSDTIVENGSTYRLGT
                     DGLQLYSSGKTQNLSVNVGGRAEVHAGTLENAVIQGGTVILLSPTSADENFVVEEDRA
                     PVELTGSVALLDGASMIIGYGADLQQSTITVQQGGVLILDGSTVKGDSVTFSVGSINL
                     NGGKLWLITGAATHVQLKVKRLRGEGAICLQTSAKEISPDFINVKGEVTGDIHVEITD
                     ASRQTLCNALKLQPDEDGIGATLQPA"
     gene            8814..8890
                     /locus_tag="ACHKBL_002889"
     tRNA            8814..8890
                     /locus_tag="ACHKBL_002889"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8848..8850,aa:Val,seq:gac)
     gene            8895..8971
                     /locus_tag="ACHKBL_002890"
     tRNA            8895..8971
                     /locus_tag="ACHKBL_002890"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8929..8931,aa:Val,seq:gac)
     gene            9079..9384
                     /locus_tag="ACHKBL_002891"
     CDS             9079..9384
                     /locus_tag="ACHKBL_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HFO5819737.1"
                     /translation="MATLLQLHFSFNGPFGDAMAEQLKPLAESINQEPGFLWKVWTES
                     EKNHEAGGIYLFTNEKSALAYLEKHTARLKNLGVEEVVAKVFDVNEPLSQINQAKLA"
     gene            9510..11120
                     /locus_tag="ACHKBL_002892"
     CDS             9510..11120
                     /locus_tag="ACHKBL_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001602606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-NAD(P)-binding protein"
                     /protein_id="HFO5819738.1"
                     /translation="MKKIAIVGAGPTGIYTLFSLLQQQTPLSISIFEQADEAGVGMPY
                     SDEENSKMMLANIASIEIPPIYCTYLEWLQKQEASHLQRYGVKKETLHDRQFLPRILL
                     GEYFRDQFLRLVDQARQQKFAVAVYESCQVTDLQITNAGVMLATNQDLPSETFDLAVI
                     ATGHVWPDEEEATRTYFPSPWSGLMEAKVDACNVGIMGTSLSGLDAAMAVAIQHGSFI
                     EDDKQHVVFHRDNASEKLNITLMSRTGILPEADFYCPIPYEPLHIVTDQALNAEIQKG
                     EVGLLDRVFRLIVEEIKFADPDWSQRIALESLNVDSFAQAWFAERKQRDPFDWAEKNL
                     QEVERNKREKHTVPWRYVILRLHEAVQEIVPHLNEHDHKRFSKGLARVFIDNYAAIPS
                     ESIRRLLALREAGIIHILALGEDYEMEINESRTVLKTEDNSYSFDVFIDARGQRPLKV
                     KDIPFPGLREQLQKTGDEIPDVGEDYTLQQPEEIRGRVAFGALPWLMHDQPFVQGLTA
                     CAEIGEAIAQAVVKPASRARRRLSFNQM"
     gene            complement(11126..11938)
                     /gene="ydhT"
                     /locus_tag="ACHKBL_002893"
     CDS             complement(11126..11938)
                     /gene="ydhT"
                     /locus_tag="ACHKBL_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdhT"
                     /protein_id="HFO5819739.1"
                     /translation="MIITRADLREWRIGAVMYRWFLRHFPRGGSYADIHHALIEEGYT
                     DWAESLVEYAWKKWLADENFAHQEVSSMQKLATDPGERAFCSQFARSDDHARIGCCED
                     NARIATAGYAAQIASMGYSVRIGSVGFNSHIGSSGERARVAVTGNSSRISSAGDSSRI
                     ANTGMRVRVCTLGERCHVASNGDLVQIASFGANARIANSGDNVHIIASGEDSTIVSTG
                     VVDSIILGPGGSAALAYHDGERVRFAVAIEGENNIRAGVRYRLNEQHQFVEC"
     gene            complement(11942..12727)
                     /gene="phsC"
                     /locus_tag="ACHKBL_002894"
     CDS             complement(11942..12727)
                     /gene="phsC"
                     /locus_tag="ACHKBL_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate reductase cytochrome B subunit"
                     /protein_id="HFO5819740.1"
                     /translation="MNPSQHAEQFQSQLANYVPLFTPQFWPVWLIIAGLLLVGMWLVL
                     GLHALLRARGVKKSANDHGEKVYLYSKAVRLWHWSNALLFVLLLASGLINHFALVGAT
                     TVKSLVAVHEVCGFLLLACWLGFVLINAVGGNGHHYRIRRQGWLERAAKQTRFYLFGI
                     MQGEEHPFPATTQSKFNPLQQVAYVGVMYGLLPLLLLTGLLCLYPQAVGDMFPGVRYW
                     LLQAHFALAFISLFFIFGHLYLCTTGRTPHETFKSMVDGYHRH"
     gene            complement(12724..13392)
                     /locus_tag="ACHKBL_002895"
     CDS             complement(12724..13392)
                     /locus_tag="ACHKBL_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416186.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFO5819741.1"
                     /translation="MSFTRRKFVLGMGTVIFFTGSASSLLANTMQEKKVRYAMIHDES
                     RCNGCNICARACRKTNHVPAQGSRLSIAHIPVTDNDNETQYHFFRQSCQHCEDAPCID
                     VCPTGASWRDEQGIVRVEKSQCIGCSYCIGACPYQVRYLNPVTKVADKCDFCAESRLA
                     KGFPPICVSACPEHALIFGREDSPEIQAWLQENKYYQYQLPGAGKPHLYRRFGQHLIK
                     KENV"
     gene            complement(13456..14094)
                     /locus_tag="ACHKBL_002896"
     CDS             complement(13456..14094)
                     /locus_tag="ACHKBL_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310406.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhW family putative oxidoreductase system
                     protein"
                     /protein_id="HFO5819742.1"
                     /translation="MNHQDELPLAKVSEVDEAKRQWLQGMRHPVDTVTEPEPAEILAE
                     FIRQHSAAGQLVARAVFLSPPYSVAEEELSVLLENIKQNGDHADIACMTGSQDDYYYS
                     TQAMSENYAAMSLQVVEQDICRAIAHAVRFECQTYPRPYKVAMLMQAPYYFQEAQIEA
                     AIAAMDIAPEYADIRQVESSTAVLYLFSERFMTYGKAYGLCEWFEVEQFQNP"
     gene            complement(14107..16209)
                     /locus_tag="ACHKBL_002897"
     CDS             complement(14107..16209)
                     /locus_tag="ACHKBL_002897"
                     /EC_number="1.2.7.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase"
                     /protein_id="HFO5819743.1"
                     /translation="MANGWTGNILRVNLTTGNITLEDSSKFKSFVGGMGFGYKIMYDE
                     VPPGTKPFDEANKLVFATGPLTGSGAPCSSRVNITSLSTFTKGNLVVDAHMGGFFAAQ
                     MKFAGYDVIIIEGKAKSPVWLKIKDDKVSLEKADFLWGKGTRATTEEICRLTSPETCV
                     AAIGQAGENLVPLSGMLNSRNHSGGAGTGAIMGSKNLKAIAVEGTKGVNIADRQEMKR
                     LNDYMMTELIGANNNHVVPSTPQSWAEYSDPKSRWTARKGLFWGAAEGGPIETGEIPP
                     GNQNTVGFRTYKSVFDLGPAAEKYTVKMSGCHSCPIRCMTQMNIPRVKEFGVPSTGGN
                     TCVANFVHTTIFPNGPKDFEDKDDGRVIGNLVGLNLFDDYGLWCNYGQLHRDFTYCYS
                     KGVFKRVLPAEEYAEIRWDQLEAGDVNFIKDFYYRLAHRVGELSHLADGSYAIAERWN
                     LGEEYWGYAKNKLWSPFGYPVHHANEASAQVGSIVNCMFNRDCMTHTHINFIGSGLPL
                     KLQREVAKELFGSEDAYDETKNYTPINDAKIKYAKWSLLRVCLHNAVTLCNWGWPMTV
                     SPLKSRNYRGDLALEAKFFKAITGEDMTQEKLDLAAERIFTLHRAYTVKLMQTKDMRN
                     EHDLICSWVFDKDPQIPVFTEGTDKMDRDDMHASLTMFYKEMGWDPQLGCPTRKTLQR
                     LGLEDIAADLAAHNLLPA"
     gene            complement(16230..16856)
                     /locus_tag="ACHKBL_002898"
     CDS             complement(16230..16856)
                     /locus_tag="ACHKBL_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein"
                     /protein_id="HFO5819744.1"
                     /translation="MNPVDRPLLNIGLTRLEFLRISGKGLAGLTIAPALLSLLGCKQE
                     DIDSGTVGLINTPKGVLVTHRARCTGCHRCEISCTNFNDGSVGTFFSRIKIHRNYFFG
                     DNGVGSGGGLYGDLNYTADTCRQCKEPQCMNVCPIGAITWQQKEGCITVDHKRCIGCS
                     ACTTACPWMMATVNTESKKSSKCVLCGECANACPTGALKIIEWKDITV"
BASE COUNT         4120 a         4410 c         4013 g         4467 t
ORIGIN      
        1 tcatttataa tgataagtgt ttttaccact tccccttttc gtcaagatcg gccaaaattc
       61 cacgcttaca ctatttgcgt actggccatt gaccccttcc tgacgctccg tgtcgttttt
      121 ccggcgtacc gcaacacttt tgttgtgcgt aaggtgtgta aaggcaaacg tttaccttgc
      181 gattttgcag gagctgaagt tagggtctgg agtgaaatgg aatggcaaca ataaaagatg
      241 tagcgaaacg agcaaacgtt tccactacaa ctgtgtcaca cgtgatcaac aaaacacgtt
      301 tcgtcgctga agaaacgcgc aacgccgtgt gggcagcgat taaagaatta cactactccc
      361 ctagcgcggt ggcgcgtagc ctgaaggtta accacaccaa gtctatcggt ttgctggcga
      421 ccagcagcga agcggcctat tttgccgaga tcattgaagc ggttgaaaaa aattgcttcc
      481 agaaaggtta caccctgatt ctgggcaatg cgtggaacaa tcttgagaaa cagcgggctt
      541 atctgtcgat gatggcgcaa aaacgcgtcg atggtctgct ggtgatgtgt tctgagtacc
      601 cagagccgtt gctggcgatg ctggaagagt atcgccatat cccaatggtg gtgatggact
      661 ggggtgaagc aaaagctgac ttcaccgatg cggtcattga taacgcattc gaaggcggct
      721 acatggccgg gcgttatctg attgaacgcg gtcatcgcga aatcggcgtt atccctggcc
      781 cgctggaacg taacaccggc gcaggccgcc ttgccggttt tatgaaggcg atggaagaag
      841 cgatgatcaa agtgccggaa agctggattg tacagggtga ctttgaacct gaatccggtt
      901 atcgcgccat gcagcaaata ctgtcgcaac cgcatcgccc tactgccgtc ttctgtggtg
      961 gcgatatcat ggcaatgggc gcactttgtg ctgctgatga aatgggtctg cgcgtgccgc
     1021 aggatgtttc gctgatcggt tatgataacg tgcgcaacgc ccgctatttt acgccagcgc
     1081 tgaccacgat ccatcagcca aaagattcgc tgggcgaaac ggcgttcaac atgctgttgg
     1141 atcgtatcgt caataaacgc gaagaaccgc agtccattga agtgcatccg cgcttgattg
     1201 aacgccgctc cgtggctgac ggcccgttcc gcgactatcg tcgttaatca cccgttgcgg
     1261 gagtctcttc cggctcccgc agccactcct tattcagcgt ctcactatcg ccgagatact
     1321 caagcaacca ggttaacgca ggcgacatat cattttgctg ccatgtcaga caacatgccg
     1381 aatccgggaa ggggttttcc agttctagtg ctacccactt cccctcatta agccacggtt
     1441 tggcgaaatg tgttggcacc atccctatgc ataatcctgc cgagatacag gttgccgatg
     1501 attcccagtc aggcacgaca agtctttttt ggttatccag caaccaggta atacgtttag
     1561 gtagcgttcg cgaggtgtct tcgcgcacca acgacggcca gttgcgcaac gtatcatcgc
     1621 tgaacgggcc atccatcaac gccagcgggt ggtggctggc aacaacgcaa ctccagctta
     1681 gcatccccat atcgcggaag gcataacgac cgcctaccgg aatcgcgcgt gttgcgccaa
     1741 tcgccagttc cacgcgcccg tcggaaagcg catcccagac accgttgaac acttcctgaa
     1801 agacaagaag ttcgacatca tcaaaatggc gataaaaatc aacgatcatc tgccgtgtac
     1861 gttctggcct gacaatatta tccactgcga tagctaactg accgcgccag ccgttcgcta
     1921 tctgctgaca ttgctgccgg gtgatctgca tttttttgac aacagagcgc ccttctttga
     1981 gaaaccacgc cccggcagcg gtcagttcca catcacggtg acgtcgttca aacagcggca
     2041 ccgccagcca ctcttccagc tgacgcacgg tatagctgac cgcagaagga acgcgatgca
     2101 actcctgtgc cgcagcgcta aaactaccat tacgcgctac cgcatcaaca acttcgagtg
     2161 aatattctga ccacatagtc tgcctgcaaa atttttgaaa ccagtcatca aatattaccg
     2221 tttcacaaca ctaatttcac tccctacact ttgcggcggt gtttaattga gagatttaga
     2281 gaatatacat gcaacctggg aaaagatttt tagtctggct ggcgggtttg agcgtactcg
     2341 gttttctggc aaccgatatg tatctgcctg ctttcgccgc catacaggcc gacctgcaaa
     2401 cgcctgcgtc tgctgtcagt gccagcctta gtctgttcct tgccggtttt gccgcagccc
     2461 agcttctgtg ggggccgctc tccgaccgtt atggtcgtaa accggtatta ttaatcggcc
     2521 tgacaatttt tgcgttaggt agtctgggga tgctgtgggt agaaaacgcc gctacgctgc
     2581 tggtattgcg ttttgtacag gctgtgggtg tctgcgccgc ggcagttatc tggcaagcgt
     2641 tagtgacgga ttattatcct tcacagaaag ttaaccgtat ttttgcgacc atcatgccgc
     2701 tggtgggtct atctccggca ctggctcctc tgttaggaag ctggctgctg gtccattttt
     2761 cctggcaggc gattttcgcc accctgtttg ccattaccgt ggtgctgatt ctgcctattt
     2821 tctggctcaa acccacgacg aaggcccgta acaatagtca ggatggtctg acctttaccg
     2881 acctgctacg ttctaaaacc tatcgcggca acgtgctgat atatgcggcc tgttcagcca
     2941 gtttttttgc atggctgacc ggctcaccgt tcatccttag tgaaatgggc tacagcccgg
     3001 cagttattgg tttaagttat gtcccgcaaa ctatcgcgtt tctgattggt ggttatggct
     3061 gtcgcgccgc actgcagaaa tggcaaggca agcagttatt accgtggttg ctggtactgt
     3121 ttgctgtcag cgtcattgcg acctgggctg cgggcttcat tagccatgtg tcgctagtcg
     3181 aaatcctgat cccattctgt gtgatggcga ttgccaatgg cgcgatctac ccgattgttg
     3241 tcgcccaggc gctgcgtccc ttcccacatg caaccggtcg cgccgcagcg ttgcagaaca
     3301 ctctacaact gggtctgtgc ttcctcgcaa gtctggtagt ttcctggctg atcagtatca
     3361 gcacgccatt gctcaccacc accagcgtga tgttatcaac agtagtgctg gtcgctctgg
     3421 gttacatgat gcaacgttgt gaagaagtag actgtcagaa tcatggcaat gccgaagtcg
     3481 ctcatagcga atcacactga tctatatcga tatgattata cttaggctgc taacaaaatt
     3541 ttgttgtatg attgaaatta gcggcctata ctaatttcga gttgttaaag ctacgataaa
     3601 tattatgttt ttacggggac aggatcgttc ccgactcact atggatggtc atttcggcaa
     3661 gggttcctcc tttccctctg ttctacgtcg gattatagac tcgcggtttt ttctgcgaga
     3721 tttctcacaa agcccaaaaa gcgtctacgc tgttttaagg ttctgatcac cgaccagtga
     3781 tggagaaact atgagttcat cgtgtataga agaagtcagt gtaccggatg acaactggta
     3841 ccgtatcgcc aacgaattac ttagccgtgc cggtatagcc attaacggtt ctgccccggc
     3901 ggatattcgt gtgaaaaacc ccgatttttt taaacgcgtt ctgcaggaag gatcgttggg
     3961 gttaggcgaa agttatatgg atggctggtg ggaatgtgac cgactggata tgttttttag
     4021 caaagtctta cgcgcaggtc tcgagaacca actcccccat catttcaaag acacgctgcg
     4081 tattgccggc gctcgtctct tcaatctgca gagtaaaaaa cgtgcctgga tagtcggcaa
     4141 agagcattac gatttgggta atgacttgtt cagccgcatg cttgatccct tcatgcaata
     4201 ttcctgtgct tactggaaag atgccgataa tctggaatca gcccagcagg cgaagctcaa
     4261 aatgatttgt gaaaaattgc agttaaaacc agggatgcgc gtactggata ttggctgtgg
     4321 ctggggtgga ctggcgcact acatggcgtc taattatgac gtaagcgtgg tcggcgtcac
     4381 catttctgcc gaacagcaaa aaatggctca ggaacgctgt gaaggcctgg atgtcaccat
     4441 tttgctgcaa gattatcgtg acctgaacga ccagtttgat cgtattgttt ctgtggggat
     4501 gttcgagcac gtcggaccga aaaattacga tacctatttt gcggtggtgg atcgtaattt
     4561 gaaaccggaa ggcatattcc tgctccatac tatcggttcg aaaaaaaccg atctgaatgt
     4621 tgatccctgg attaataaat atatttttcc gaacggttgc ctgccctctg tacgccagat
     4681 tgctcagtcc agcgaacccc actttgtgat ggaagactgg cataacttcg gtgctgatta
     4741 cgatactacg ttgatggcgt ggtatgaacg attcctcgcc gcatggccag aaattgcgga
     4801 taactacagt gaacgcttta aacggatgtt tacctattat ctgaatgcct gtgcgggtgc
     4861 tttccgcgcc cgtgatattc agctctggca ggtcgtgttc tcacgcggtg ttgaaaacgg
     4921 ccttcgagtg gctcgctaaa ggctattcta tcgccccctc tccgggggcg atttcagatc
     4981 aggcttctgt acctggttga ttcatggcat tttctcgtgc cgccagcact cgttctaccg
     5041 tatctaccac tgcctgagtt tgtggatcga tttcaatgtt gacgcgtgcg ccaagttttt
     5101 tcttcccaag agtcgtgcgt tccagtgttt ccggaattaa atggacgcaa aaacgcgttg
     5161 gcgtgacttc accgacggtc aggctaatac cgtcgatgcc aataaatcct ttgtacagaa
     5221 tatatttcat caactgacta tcctggactt taaaccagat ctggcgatta ttttccgagg
     5281 ttaatatttt cgccacttca gcggtggtca taatatgacc tgacattaag tgtccgccaa
     5341 tttcatcact gaatttcgcc gcacgctcaa cgtttaccca atcccccact tttaaatcgc
     5401 caagattggt aatgcgtaac gtttctttca tcaggtcaaa actgacatgg ttgccgttaa
     5461 tttccgtcac ggtcaggcag caaccgttat gcgccacgga agcaccggtt tccagaccgt
     5521 ccagcatgtg gtcgggtaac tccaccacat gtgtgcgaaa atttggtttc tcgtcaatcg
     5581 acaccagttt tgcggtgccc tgtacaatcc ccgtaaacat acttacaact cctgaaatca
     5641 gttaagacat tctgttcagc acaatagcag gtggcaaacg cccttaccag tgaaggggta
     5701 agaatgacta ttttttcact ggagaattaa taaatcctcg ctacaataga ctgaatttcc
     5761 cctgcttctt ctttttgctg cccattcagg cggcttttta gtctctcata taactacaaa
     5821 taaaaggtgt tcacgtgcag aagtatatca gtgaagcgcg tctgttatta gcattagcaa
     5881 tcccggtgat tctcgcgcaa atcgcccaaa ctgcgatggg ttttgtcgat accgtgatgg
     5941 cgggcggcta tagtgccacc gacatggcgg cggtcgctat cggtacttct atctggcttc
     6001 cggcgatcct ctttggtcac ggactgctgc tggcattaac gccggttatc gcgcaattaa
     6061 atggttccgg tcgacgtgag cgcattgcgc atcaggtgcg acaaggtttc tggctggcgg
     6121 gttttgtttc cgttctcatt atgctggtgc tgtggaatgc aggttacatt atccgctcca
     6181 tggaaaacat cgatccggct ctggcggaca aagccgtggg ttatctgcgt gcgttgctat
     6241 ggggcgcgcc gggctatctt ttcttccagg ttgcccgtaa ccagtgtgaa ggtctggcaa
     6301 aaaccaagcc gggtatggtg atgggcttta tcggcctgct ggtgaacatt ccggtgaact
     6361 atatctttat ttacggtcat ttcggtatgc ctgagctcgg tggcgttggt tgtggcgtgg
     6421 ctactgctgc ggtgtattgg gtcatgttcc ttgccatggt ttcttacatt aaacgcgccc
     6481 gctcgatgcg cgatattcgt aacgaaaaag gcactgcgaa gcccgatcct gcggttatga
     6541 aacgactgat tcaactcggt ttgccaattg cgctggcgct gttctttgaa gtaacgctgt
     6601 ttgccgtcgt ggctctgtta gtgtctccgc tcggtattgt tgatgtcgca ggacatcaga
     6661 ttgccctgaa ctttagttca ctgatgttcg tacttcccat gtcgctggcg gcagcggtaa
     6721 ccatccgcgt aggttatcgt ctgggtcagg gctcaacgct ggatgcgcaa accgctgcgc
     6781 ggaccgggct tatggtgggt gtctgtatgg caaccctgac ggccattttc acggtttcac
     6841 tgcgggagca aatcgccctg ttgtacaacg acaatcccga ggttgtaacg ctggctgcgc
     6901 atttgatgtt gctggcggcg gtatatcaga tttctgactc aatccaggtg attggcagtg
     6961 ggattttgcg tggatataaa gatacgcgtt ccattttcta tattaccttt acggcttact
     7021 gggtgctggg cttgccaagc ggctatattc tggcactgac cgatctggtc gttgaaccta
     7081 tggggccagc aggcttctgg ataggcttta ttattggcct gacgtcggca gccattatga
     7141 tgatgctgcg tatgcggttc ctgcaacgtc tgccgtcagc catcattctg caacgagcag
     7201 ctcgctagta aagacaaggc gcaaccttca cgggttgcgc ctgtgttttt acgcaggctg
     7261 gagcgttgcg ccaatcccgt cttcgtctgg ctgtagtttc agtgcgttac acagagtttg
     7321 ccgactggca tctgttatct caacgtgtat atcaccagta acttcccctt tcacattgat
     7381 gaagtcaggt gaaatttctt tcgcactggt ttgcaggcaa atcgctccct ctccgcgcag
     7441 gcgtttcact ttcagttgca catgcgttgc cgcaccagtg attagccaca gttttccgcc
     7501 atttagattg atgctaccaa cactgaaagt gacactgtca ccttttaccg tactgccgtc
     7561 gagaatcaat acaccgccct gctgtacagt aatcgttgat tgttgcagat ctgcgccata
     7621 gccaataatc attgaagcgc cgtccagtaa tgcaacacta ccggtcagtt caaccggtgc
     7681 gcgatcttcc tctacgacaa aattttcgtc cgcgctggtg ggtgacaaca ggatgactgt
     7741 cccaccttgt attaccgcat tttccagcgt accggcatgc acttcagccc gaccacccac
     7801 attaacggac aggttttgcg tcttaccgga actgtagagc tgaagaccat ccgtccccag
     7861 acgataagtg gatccattct caacaatagt gtcagagtct tcaccaccga gattattcat
     7921 ggtgccacca gcaagcaatg tatcactggc tttccccgcc agcataaaaa cgccgccctg
     7981 attaatctca caattgctgg cttccccacc tggatacacc aacaattcac caccttcatc
     8041 taccacgacc gctgaggttg tcccattgac caacaggcca ccctcttgat cgagaataat
     8101 tttttccgcg cgatggcctt ccagtacacg caggttaccg ccattttcca gcaacaaacc
     8161 gcaggcataa cctttatcaa cgctgaattc accttcggga tggcggccat taaccgtagc
     8221 gagcgtagag agactaattg cgcctccctc ggactgaaca atatctgtgg caatggcacc
     8281 gtcgctgacc gatagcgtgc cattgttgcc gtactgtgtg catttggcga tcccaccggt
     8341 tgccactttc aaccagccag cccccgtgac cacggtattt tcggctcttc cgccagcttc
     8401 aacaattaac tcaccttcag taagttgcgt cgcgccaatg acctcgccgg tcttaacaat
     8461 ttgcacgttc ccgtcgctca tcaagttttt cgcatcagat cccatgattt attcctttgc
     8521 tgcatctgtg tgcctttatt gctacctaag tgtaaaggct acggaggatt tatccacgac
     8581 agattttgag atggtggcaa acaactctgt ttaaactctg atacacggat tattgggttg
     8641 tatctgatgt aaatgcgatc ctgaataaaa atcgcccttg caaatcaaca aaatatagcc
     8701 aatttgctta aaagctctgc aaacggtcga aatagcgcag aaaattacgt tttgcctctt
     8761 gccaccttcc cactctgccg ctaatattcg tccccgttgt cacctacaac gttgcgttca
     8821 tagctcagtt ggttagagca ccaccttgac atggtggggg tcgttggttc gagtccaatt
     8881 gaacgcacca tcctgcgtcc gtagctcagt tggttagagc accaccttga catggtgggg
     8941 gtcggtggtt cgagtccact cggacgcacc agattttctt aatctggtct tctccttttt
     9001 ccctctgttt cttctctata tccaatacgt taaaagattt acactgtctt cgtatgcgtt
     9061 atcagaagga gaatcgctat ggcaactttg ttacaacttc atttttcttt caatggccca
     9121 tttggcgacg caatggctga gcagcttaaa ccgcttgctg agtcgattaa tcaggaacct
     9181 ggttttctgt ggaaggtatg gacagaaagt gaaaagaacc acgaagccgg tgggatctac
     9241 ctttttacta atgaaaaaag cgcccttgcc tatctggaaa aacatactgc ccgactgaaa
     9301 aatctcggcg ttgaggaagt tgtcgccaaa gttttcgatg tcaatgagcc acttagtcaa
     9361 atcaatcagg caaaactcgc ctgacagaat ttaatcaagg gcggttagcg cccttttcat
     9421 ccctgtctga aatttctcaa attctaaaaa tctcaaccaa acttatctga taacactaaa
     9481 ttcgaaagaa tgcgtacagg taagtaacaa tgaaaaaaat tgctattgtg ggtgccgggc
     9541 ctacggggat ctacacctta ttctcgcttc tacagcaaca aactccactt tctatttcta
     9601 tcttcgagca ggctgatgaa gccggtgtcg ggatgccata cagtgatgag gaaaactcaa
     9661 aaatgatgct ggcaaatatt gccagtattg aaataccgcc gatttattgt acgtatctcg
     9721 aatggctaca aaagcaagaa gccagccatc tccagcgtta tggcgttaaa aaagaaacct
     9781 tgcacgatcg tcagttttta ccgcgaattc tgctgggcga atatttccgc gatcaatttt
     9841 tacgattagt agaccaggca cgacagcaaa aatttgcagt ggctgtttat gaatcatgcc
     9901 aggttaccga tctgcaaatt acaaatgctg gcgtcatgct cgctacaaat caggatttac
     9961 ccagcgagac gtttgattta gcggtgatcg ccacgggtca cgtctggcct gatgaagaag
    10021 aagcaacccg aacgtatttt cccagcccgt ggtcaggcct gatggaagca aaggtcgatg
    10081 cgtgtaacgt gggtattatg ggaacatcct tgagcggact ggatgcggca atggcagtgg
    10141 ctattcagca tggttcgttc attgaagatg ataaacaaca cgtcgttttt caccgcgata
    10201 acgcaagtga aaagctaaat attacgttga tgtcgcgtac gggtatttta cccgaagccg
    10261 atttctattg ccctattccc tacgagccct tacacatcgt caccgatcaa gcattaaatg
    10321 ctgagattca aaaaggcgaa gtgggccttt tggatcgggt atttagattg atagtagagg
    10381 aaatcaaatt tgctgatcca gactggagcc aacgcatagc cttagaaagc ctgaatgtcg
    10441 attcctttgc tcaagcctgg tttgccgagc gcaaacaacg cgacccattc gactgggcag
    10501 aaaaaaatct ccaggaagtc gaacgcaata aacgagaaaa acatactgtt ccctggcgtt
    10561 atgtcattct gcgcctgcat gaagccgtac aggaaattgt tcctcatctg aatgaacacg
    10621 accataaacg gttcagtaaa ggccttgccc gggttttcat cgataattat gcggcaatcc
    10681 cttcagagtc tattcgtcga ctactggcct tacgtgaagc gggaatcatt catattctcg
    10741 ccctcggtga agactacgaa atggaaatta atgagtcgcg caccgtcctg aaaacggaag
    10801 acaatagcta ctcgtttgac gtttttattg atgcccgtgg gcagcgtccg cttaaagtga
    10861 aagatattcc tttccctgga ctacgcgaac aattacagaa aacaggggat gaaatccctg
    10921 atgttggcga agattatacg ctacagcaac ccgaagaaat tcgtgggcgc gtagcgttcg
    10981 gcgcgttgcc ctggttgatg cacgaccagc cattcgtaca gggacttacg gcatgtgcag
    11041 aaattggtga ggcgatagct caggcggtcg taaagcctgc atcccgtgct cgtcggcgtc
    11101 tttcgtttaa tcaaatgtaa agctatcagc actcaacaaa ctgatgctgt tcattaaggc
    11161 gatagcggac tccggctcga atattgtttt caccttcaat ggcgacggcg aagcgaactc
    11221 tttcgccgtc atgatatgcc agtgcggcgc ttccccctgg cccgagaata attgagtcga
    11281 ccacgcccgt gctcactatt gttgaatctt caccgctggc aatgatgtgt acattgtcgc
    11341 cgctgttggc aattctggcg tttgcgccaa agctggcgat ttgcaccagg tcaccattac
    11401 tggcgacatg acatcgttca cccaatgtac aaacgcgtac tcgcattccg gtattcgcaa
    11461 tccgactgct atctccagcg ctgcttatcc gcgaagaatt accggttaca gcaacccgtg
    11521 cgcgttcacc ggagctgcca atgtgactgt taaagccgac acttccaatc cgcacgctat
    11581 aacccatact ggcaatttgc gcggcatacc cagcggttgc gattctggca ttatcctcac
    11641 aacagccgat gcgagcatga tcgtcgctgc gggcaaactg actgcaaaat gctctctctc
    11701 cgggatctgt ggccagtttt tgcatagaag aaacttcctg atgcgcgaag ttttcatccg
    11761 ccagccattt tttccacgca tactcaacca gactctccgc ccagtccgta tatccttcct
    11821 caatcagtgc atgatgaata tcggcataac taccgccgcg tgggaaatgg cgcagaaacc
    11881 agcgatacat caccgctccg atacgccatt ctcgcaggtc tgctcgtgta atgatcattc
    11941 gttagtgccg gtgatagcca tcgaccatgc ttttaaaggt ttcgtgtggc gtacgccccg
    12001 tggtgcaaag ataaagatga ccgaagataa aaaagaggct tataaatgcc agagcaaaat
    12061 gcgcctgcaa taaccagtat cttacgccag gaaacatatc tcccacggct tgcggataga
    12121 gacacagcag ccccgtcaat agtaacaatg gcagcaatcc atacatgaca ccaacatagg
    12181 cgacctgctg taagggatta aatttagact gggttgttgc cgggaaagga tgttcttccc
    12241 cctgcataat gccaaacaaa tagaatcgcg tttgttttgc cgctcgctcc agccacccct
    12301 gacgacgaat gcgatagtgg tgaccattac ccccaacggc attgatcaga acaaagccga
    12361 gccagcatgc cagtaacaaa aatccgcaaa cttcatgcac cgcaaccaga cttttaaccg
    12421 tagttgcgcc caccagcgca aagtgattta tcagcccact tgccagcaac aatacaaaga
    12481 gtaacgcatt cgaccagtgc cataatctga ccgctttgct gtaaagataa accttctcac
    12541 catggtcgtt ggctgatttc ttcacgccac gagcacgaag caaggcatgc agccccagca
    12601 ccaaccacat ccctaccagc aatagtccgg caatgatcaa ccacaccggc caaaattggg
    12661 gagtgaacag tggcacatag ttcgccaact ggctctgaaa ctgttcagca tgttgcgacg
    12721 ggttcataca ttttcctttt taatcaaatg ttgaccgaac cggcgataca ggtgcggttt
    12781 cccggcacca ggtagctgat attgatagta tttattttcc tgcaaccacg cctgaatttc
    12841 tgggctatct tcgcgaccaa agatcaatgc gtgttccgga caggcgctga cgcaaatggg
    12901 cggaaagcct tttgccagtc gggactcagc gcagaaatca catttatctg ccactttagt
    12961 gacagggtta aggtagcgaa cctggtaagg acaagcgcca atgcaataac tgcagccgat
    13021 gcactgcgat ttttctaccc gcacgatccc ctgctcatca cgccacgacg cccctgtcgg
    13081 gcaaacgtcg atgcaaggag catcttcaca gtgctggcat gactggcgaa aaaagtgata
    13141 ctgcgtttcg ttgtcgttat cggtgactgg aatgtgtgct atcgataaac ggcttccctg
    13201 agcggggacg tggttagttt tacggcatgc gcgggcgcaa atattacagc cattgcaccg
    13261 tgactcgtca tgaatcatgg cataccgaac tttcttttct tgcatcgtgt tcgctaacag
    13321 agaagacgca gaaccagtaa aaaagattac tgttcccatc cccagaacaa atttgcgtcg
    13381 agtgaaggac atctgattat cccctaatgc agtggctgct actgtccacc ctatccatga
    13441 catctccctg aaatttcagg ggttctgaaa ctgctctacc tcgaaccatt cgcataagcc
    13501 atacgccttg ccataggtca taaatcgctc gctaaacaag tacaacaccg ccgtcgatga
    13561 ctccacctgc cgtatatcag catattcagg tgcaatatcc atcgcggcga tcgcggcttc
    13621 gatctgcgct tcctggaagt aatatggcgc ttgcatcagc atggcgactt tgtaaggacg
    13681 aggatacgtc tggcactcaa aacgaacggc gtgtgcaatg gcgcgacaaa tgtcctgctc
    13741 cacaacttgc aaagacatcg ccgcgtagtt ttcactcatg gcctgcgttg agtaatagta
    13801 atcatcctgg cttcccgtca tacacgcgat atccgcgtgg tctccgttct gcttaatgtt
    13861 ttccagcaaa actgacagtt cctcttctgc aaccgaatag ggcggcgaga gaaacacggc
    13921 gcgcgccacc agctgcccag ccgcagaatg ctgacgaatg aactctgcca ggatttcagc
    13981 aggttctggt tcggttaccg tatcaacggg gtgacgcatc ccctgcaacc attgccgctt
    14041 cgcctcatca acttcgctga ctttcgctaa cggcaattca tcctgatggt tcatctcacc
    14101 cattccttac gcaggcagta gattgtgtgc tgccagatcg gcggcaatat cttccagccc
    14161 cagacgctgc aatgttttgc gggtcggaca accaagctgt gggtcccagc ccatttcttt
    14221 gtagaacatc gtgagcgaag catgcatatc gtcacggtcc attttgtctg taccttcagt
    14281 aaagaccggg atctgcggat ccttgtcgaa tacccaggaa cagataagat cgtgttcatt
    14341 acgcatatct ttagtttgca tcagttttac cgtgtaggcg cgatgcaacg taaagatacg
    14401 ctctgcagct aaatctaatt tttcctgagt catatcttcg ccagtgatcg ctttgaagaa
    14461 tttggcttca agcgccagat cgccccgata attacggctt ttcagcggcg aaacggtcat
    14521 tggccagccc cagttgcaca gggtgacggc gttatgcaaa cagacccgca acagcgacca
    14581 cttagcatat ttaatttttg cgtcattgat tggcgtgtag tttttggttt catcgtaagc
    14641 atcttcagaa ccaaaaagtt ctttcgccac ttcacgttgc agtttcaatg gcaagccgga
    14701 accaataaag ttgatatggg tatgcgtcat gcagtcacgg ttgaacatac agttaacaat
    14761 ggagccgacc tgcgctgacg cttcattggc gtggtgaact ggatagccaa acggcgacca
    14821 gagtttattt ttcgcgtagc cccagtactc ttcacccaaa ttccagcgtt ctgcgatggc
    14881 atatgaacca tcagccaggt gactcagctc acccacgcga tgcgccagac ggtagtaaaa
    14941 atctttaatg aagttaacgt cacccgcttc cagttgatcc cagcgaattt ccgcatactc
    15001 ttcagcaggc agaacacgct tgaacacccc tttgctgtaa caataggtaa agtcgcgatg
    15061 aagctgacca tagttacacc ataggccgta gtcatcgaac agattcagac caaccaggtt
    15121 accaatcaca cgaccatcat ctttatcttc aaaatctttc ggaccgttcg ggaagatggt
    15181 ggtatggaca aagtttgcta cacaagtgtt accacctgtg ctgggcacgc caaactcttt
    15241 cacccgagga atattcattt gggtcataca acggatcggg caagagtggc agccactcat
    15301 ttttactgtg tatttctctg ccgccggacc taagtcaaaa acggatttat aggtacgaaa
    15361 gccgaccgtg ttctgattgc ctggcggaat ctcacccgtt tcaatcggac caccttcagc
    15421 cgcgccccaa aacaaccctt tacgtgcagt ccaacgtgac ttgggatctg aatactctgc
    15481 ccacgattgc ggcgtacttg gcacgacatg gttgttattc gcaccgataa gttcagtcat
    15541 catgtaatcg ttgagacgct tcatctcctg acgatcggca atgttgaccc ctttcgtccc
    15601 ttcaaccgca atcgctttca ggtttttcga acccattatt gcgccagttc ccgcaccacc
    15661 gctgtggtta cggctattca acatgccaga gagaggaaca aggttttccc cagcctgacc
    15721 aatagccgcc acacaggttt ccggactggt caagcgacaa atttcttccg tcgttgcgcg
    15781 cgtccctttt ccccataaga aatcggcttt ttcaaggcta actttgtcat ctttaatctt
    15841 cagccatacc ggtgatttcg ctttcccttc gataataatg acgtcgtatc cagcgaattt
    15901 catttgcgct gcaaaaaagc cccccatatg ggcatcgacg actaaatttc ctttggtaaa
    15961 agtagaaagt gaggtgatat ttacgcgaga actacagggg gcaccagatc cagttaatgg
    16021 gccggtagca aaaactaatt tattcgcttc atcgaaaggt ttcgtgcctg gcggtacttc
    16081 gtcatacata attttgtagc cgaagcccat gccaccgaca aaacttttaa acttactgga
    16141 atcttcgagg gtaatatttc ctgtcgtgag attgactctt aatatattac ctgtccaacc
    16201 gttagccatg attttttcct ttgcaagatt cacacagtaa tatctttcca ctcgataatt
    16261 tttaacgccc ctgttgggca ggcgtttgcg cattcaccgc ataacacaca tttcgaggat
    16321 tttttacttt cggtatttac ggtggccatc atccacgggc atgccgtagt acaggcgcta
    16381 cagccaatac aacgtttatg atcgacggta atacagcctt ctttctgctg ccaggtaatc
    16441 gcaccaatcg ggcagacgtt catgcattgc ggctctttgc attgacggca ggtgtccgcg
    16501 gtatagttga gatcgccata caggccgcca ccagagccaa ccccgttatc gccaaagaaa
    16561 taattgcgat ggattttgat acgggagaag aatgtcccta ctgagccatc gttgaagttg
    16621 gtacaagaga tttcacagcg gtggcaaccc gtgcatcgcg cacgatgggt caccagcacc
    16681 cctttgggtg tatttatcaa gcctactgtg ccgctatcaa tatcttcctg tttgcaaccc
    16741 aaaagtgaca gtaacgcagg ggcaattgtt aaaccagcaa ggccttttcc tgatatacgc
    16801 aagaattcta atcgcgttaa gccaatattt aatagtggac gatcaaccgg gttcatttat
    16861 ttcgcatcct cttcgcaaac acagtgctga caattcagca gcctgatatt aggcactcaa
    16921 ctaatacgct gctgactctg tcctcaacac cagatatttt ccctaaccca atagtgtatg
    16981 agaaaagatt taacgctgat aaactaatcc
//
LOCUS       DAZFLD010000107        16927 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.108, whole genome shotgun sequence.
ACCESSION   DAZFLD010000107 DAZFLD010000000
VERSION     DAZFLD010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16927)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16927
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.108"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(23..718)
                     /gene="araD"
                     /locus_tag="ACHKBL_002899"
     CDS             complement(23..718)
                     /gene="araD"
                     /locus_tag="ACHKBL_002899"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFO5819745.1"
                     /translation="MLEDLKRQVLEANLALPKHNLITLTWGNVSAVDRERGVLVIKPS
                     GVDYNVMTADDMVVVSIETGEVVEGTKKPSSDTPTHRLLYQAFPSIGGIVHTHSRHAT
                     IWAQAGQSIPATGTTHADYFYGTIPCTRKMTDAEINGEYEWETGNVIVETFEKQGIDA
                     AQMPGVLVHSHGPFAWGKNAEDAVHNAIVLEEVAYMGIFCRQLAPQLPDMQQTLLDKH
                     YLRKHGAKAYYGQ"
     gene            complement(918..2420)
                     /gene="araA"
                     /locus_tag="ACHKBL_002900"
     CDS             complement(918..2420)
                     /gene="araA"
                     /locus_tag="ACHKBL_002900"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HFO5819746.1"
                     /translation="MTIFDNYEVWFVIGSQHLYGPETLRQVTQHAEHVVNALNTEAKL
                     PCKLVLKPLGTTPDEITAICRDANYDDRCAGLVVWLHTFSPAKMWINGLTMLNKPLLQ
                     FHTQFNAALPWDSIDMDFMNLNQTAHGGREFGFIGARMRQQHAVVTGHWQDKQAHERI
                     GSWMHQAVSKQDTRHLKVCRFGDNMREVAVTDGDKVAAQIKFGFSVNTWAVGDLVQVV
                     NSISDGDVNALVDEYESCYTMTPATQIHGEKRQNVLEAARIELGIKRFLEQGGFHAFT
                     TTFEDLHGLKQLPGLAVQRLMQQGYGFAGEGDWKTAALLRIMKVMSTGLQGGTSFMED
                     YTYHFEKGNDLVLGSHMLEVCPSIAVEEKPILDVQHLGIGGKDDPARLIFNTQTGPAI
                     VASLIDLGDRYRLLVNCIDTVKTPHSLPKLPVANALWKAQPDLPTASEAWILAGGAHH
                     TVFSHALNLNDMRQFAEMHDIEITVIDNDTRLPAFKDALRWNEVYYGFRR"
     gene            complement(2431..4131)
                     /gene="araB"
                     /locus_tag="ACHKBL_002901"
     CDS             complement(2431..4131)
                     /gene="araB"
                     /locus_tag="ACHKBL_002901"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008741 - ribulokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HFO5819747.1"
                     /translation="MAIAIGLDFGSDSVRALAVDCATGEEIATSVEWYPRWQKGQFCD
                     APNNQFRHHPRDYIESMEAALKTVLAELSAEQRAAVVGIGVDTTGSTPAPIDADGNVL
                     ALRPEFAENPNAMFVLWKDHTAVEEAEEITRLCHTPGNVDYSRYIGGIYSSEWFWAKI
                     LHVTRQDSAVAQSAASWIELCDWVPALLSGTTRPQDIRRGRCSAGHKSLWHESWGGLP
                     PASFFDELDPILNRHLPSPLFTDTWTADIPVGTLCPEWAQRLGLPESVVISGGAFDCH
                     MGAVGAGAQPNALVKVIGTSTCDILIANKQSVGERAVKGICGQVDGSVVPGFIGLEAG
                     QSAFGDIYAWFGRVLGWPLEQLAAQHPELKEQINASQKQLLPALTEAWAKNPSLDHLP
                     VVLDWFNGRRTPNANQRLKGVITDLNLATDAPLLFGGLIAATAFGARAIMECFTDQGI
                     AVNNVMALGGIARKNQVIMQACCDVLNRPLQIVASDQCCALGAAIFAAVAAKVHADIP
                     SAQQKMASAVEKTLQPRSEQAQRFEQLYRRYQQWAMSAEQHYLPTSAPAQAAQAVPTL
                     "
     gene            4470..5348
                     /gene="araC"
                     /locus_tag="ACHKBL_002902"
     CDS             4470..5348
                     /gene="araC"
                     /locus_tag="ACHKBL_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HFO5819748.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKG
                     YILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRA
                     YWHEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLL
                     LRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQ
                     LGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEF
                     RAGCEEKVNDVAAKLS"
     gene            5434..6198
                     /gene="yabI"
                     /locus_tag="ACHKBL_002903"
     CDS             5434..6198
                     /gene="yabI"
                     /locus_tag="ACHKBL_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFO5819749.1"
                     /translation="MQALLEHFITQSTVYSLMAVVLVAFLESLALVGLILPGTVLMAG
                     LGALIGSGELSFWHAWLAGIVGCLLGDWISFWLGWRFKKPLHRWSFLKKNKALLDKTE
                     HALHQHSMFTILVGRFVGPTRPLVPMVAGMLDLPVAKFITPNIIGCLLWPPFYFLPGI
                     LAGAAIDIPAGMQSGEFKWLLLATAVFLWVGGWLCWRLWRSGKATDRLSHYLSRGRLL
                     WLAPLISAVGVVALVVLIRHPLMPVYIDILRKVVGV"
     gene            complement(6312..7010)
                     /gene="thiQ"
                     /locus_tag="ACHKBL_002904"
     CDS             complement(6312..7010)
                     /gene="thiQ"
                     /locus_tag="ACHKBL_002904"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein
                     ThiQ"
                     /protein_id="HFO5819750.1"
                     /translation="MLKLTDITWLYHHLPMRFSLTVERSEQVAILGPSGAGKSTLLNL
                     IAGFLTPASGSLTIDGVDHTTTPPSRRPVSMLFQENNLFSHLTVAQNIGLGLNPGLKL
                     NAAQQEKMHAIARQMGIDNLMARLPGELSGGQRQRVALARCLVREQPILLLDEPFSAL
                     DPALRQEMLTLVSTSCQQQKMTLLMVSHSVEDAARIATRSVVVADGRIAWQGMTDELL
                     SGKASASAILGITG"
     gene            complement(6994..8604)
                     /gene="thiP"
                     /locus_tag="ACHKBL_002905"
     CDS             complement(6994..8604)
                     /gene="thiP"
                     /locus_tag="ACHKBL_002905"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease ThiP"
                     /protein_id="HFO5819751.1"
                     /translation="MATRRQPLIPGWLIPGISAATLVVAVALAAFLALWWNAPQGDWV
                     AIWQDSYLWHVVRFSFWQAFLSALLSVVPAIFLARALYRRRFPGRLALLRLCAMTLIL
                     PVLVAVFGILSVYGRQGWLASLWQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQ
                     ALENIPGEQRQLAAQLGMRGWHFFRFVEWPWLRRQIPPVAALIFMLCFASFATVLSLG
                     GGPQATTIELAIYQALSYDYDPARAAMLALIQMVCCLGLVLLSQRLSKAIAPGTTLLQ
                     GWRDPDDRLHSRICDTVLIVLALLLLLPPLLAVLVDGINRQLPEVLAQPVLWQALWTS
                     LRIALAAGVLCVVLTMMLLWSSRELRARQKMLAGQALEMSGMLILAMPGIVLATGFFL
                     LLNNTIGLPQSADGIVIFTNALMAIPYALKVLENPMRDITARYSMLCQSLGIEGWSRL
                     KVVELRALKRPLAQALAFACVLSIGDFGVVALFGNDDFRTLPFYLYQQIGSYRSQDGA
                     VTALILLLLCFLLFTVIEKLPGRNVKTD"
     gene            complement(8580..9563)
                     /gene="thiB"
                     /locus_tag="ACHKBL_002906"
     CDS             complement(8580..9563)
                     /gene="thiB"
                     /locus_tag="ACHKBL_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0030975 - thiamine binding [Evidence IEA];
                     GO_function: GO:0048502 - ABC-type thiamine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HFO5819752.1"
                     /translation="MLKKCLPLLLLCTAPVFAKPVLTVYTYDSFAADWGPGPKIKKAF
                     EADCNCELKLVALEDGVSLLNRLRMEGKNSKADVVLGLDNNLLDAASKTGLFAKSGVA
                     ADAVNVPGGWKNDTFVPFDYGYFAFVYDKNKLKNPPQSLKELVESDQNWRVIYQDPRT
                     STPGLGLLLWMQKVYGDDAPQAWQKLAKKTVTVTKGWSEAYGLFLKGESDLVLSYTTS
                     PAYHILEEKKDNYAAANFSEGHYLQVEVAARTAASKQPELAQKFLQFMVSPAFQNAIP
                     TGNWMYPVANVTLPAGFEQLTKPATTLEFTPAEVAAQRQAWISEWQRAVSR"
     regulatory      complement(9594..9693)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(9727..11382)
                     /gene="sgrR"
                     /locus_tag="ACHKBL_002907"
     CDS             complement(9727..11382)
                     /gene="sgrR"
                     /locus_tag="ACHKBL_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SgrR"
                     /protein_id="HFO5819753.1"
                     /translation="MPSARLQQQFIRLWQCCEGKSQDTTLNELAALLSCSRRHMRTLL
                     NTMQDRGWLTWEAEVGRGKRSRLTFLYTGLALQQQRAEDLLEQDRIDQLVQLVGDKAA
                     VRQMLVSHLGRSFRQGRHILRVLYYRPLRNLLPGSALRRSETHIARQIFSSLTRINEE
                     NGELEADIAHHWQQILPLHWRFFLRPGVHFHHGRELEMDDVIASLKRINTLPLYSHIA
                     DIVSPTPWTLDIHLTQPDRWLPLLLGQVPAMILPREWETLSNFASHPIGTGPYAVIRN
                     SSNQLKIQAFDDFFGYRALIDEVNVWVLPEIADDPAGGLMLKGPQDEEKEIESRLEEG
                     CYYLLFDSRTHRGANQQVRDWISYVLSPTNLVYFAEEQYQQLWFPAYGLLPRWHHTRT
                     IKSEKPAGLESLTLTFYQDHSEHRVIAGIMQQILASHQVTLEIKEISYDQWHEGEIES
                     DIWLNSANFTLPLDFSLFAHLCEVPLLQHCIPIDWQADAARWRNGEINLANWCQQLVA
                     SKAMVPLIHHWLIIQGQRSMRGLRMNTLGWFDFKSAWFAPPDP"
     gene            11471..11602
                     /gene="sgrT"
                     /locus_tag="ACHKBL_002908"
     CDS             11471..11602
                     /gene="sgrT"
                     /locus_tag="ACHKBL_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001248776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046325 - negative regulation of glucose
                     import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake inhibitor SgrT"
                     /protein_id="HFO5819754.1"
                     /translation="MRQFYQHYFTATAKLCWLRWLSVPQRLTMLEELMQWEGSHSDY"
     gene            complement(11710..12315)
                     /gene="leuD"
                     /locus_tag="ACHKBL_002909"
     CDS             complement(11710..12315)
                     /gene="leuD"
                     /locus_tag="ACHKBL_002909"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HFO5819755.1"
                     /translation="MAEKFIKHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLF
                     NDWRFLDEKGQQPNPDFVLNFPQYQGASILLARENFGCGSSREHAPWALTDYGFKVVI
                     APSFADIFYGNSFNNQLLPVKLSDAEVDELFALVQANPGIHFDVDLEAQEVKAGEKTY
                     RFTIDAFRRHCMMNGLDSIGLTLQHDDAIASYEEKQPAFMR"
     gene            complement(12326..13726)
                     /gene="leuC"
                     /locus_tag="ACHKBL_002910"
     CDS             complement(12326..13726)
                     /gene="leuC"
                     /locus_tag="ACHKBL_002910"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HFO5819756.1"
                     /translation="MAKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFDGL
                     RAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNH
                     PYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
                     GRAKTMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMT
                     LCNMAIEMGAKAGLVAPDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTV
                     VTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVERASAEKALAYMGLKPGIPL
                     TEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGPVKAQAEAEGLD
                     KIFIEAGFEWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMA
                     AAAAVTGHFADIRNIK"
     gene            complement(13729..14820)
                     /gene="leuB"
                     /locus_tag="ACHKBL_002911"
     CDS             complement(13729..14820)
                     /gene="leuB"
                     /locus_tag="ACHKBL_002911"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HFO5819757.1"
                     /translation="MSKNYHIAVLPGDGIGPEVMTQALKVLDAVRNRFAMRITTSHYD
                     VGGAAIDNHGQPLPPATVEGCEQADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKHF
                     KLFSNLRPAKLYQGLEAFCPLRADIAANGFDILCVRELTGGIYFGQPKGREGSGQYEK
                     AFDTEVYHRFEIERIARIAFESARKRRHKVTSIDKANVLQSSILWREIVNEIATEYPD
                     VELAHMYIDNATMQLIKDPSQFDVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQG
                     FGLYEPAGGSAPDIAGKNIANPIAQILSLALLLRYSLDADDAACAIERAINRALEEGI
                     RTGDLARGAAAVSTNEMGDIIARYVVEGV"
     gene            complement(14820..16391)
                     /gene="leuA"
                     /locus_tag="ACHKBL_002912"
     CDS             complement(14820..16391)
                     /gene="leuA"
                     /locus_tag="ACHKBL_002912"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HFO5819758.1"
                     /translation="MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVME
                     VGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIAT
                     SPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAIN
                     AGATTINIPDTVGYTMPFEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAV
                     HAGARQVEGAMNGIGERAGNCSLEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQI
                     CNMPIPANKAIVGSGAFAHSSGIHQDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGR
                     AAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIGKQQEEPEHFR
                     LDYFSVQSGSNDIATAAVKLACGEEVKAEAANGNGPVDAVYQAINRITDYNVELVKYS
                     LTAKGHGKDALGQVDIVANYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRAAEVEKE
                     LQRKAQHNENNKETV"
     gene            complement(16484..16570)
                     /gene="leuL"
                     /locus_tag="ACHKBL_002913"
     CDS             complement(16484..16570)
                     /gene="leuL"
                     /locus_tag="ACHKBL_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leu operon leader peptide"
                     /protein_id="HFO5819759.1"
                     /translation="MTHIVRFIGLLLLNASSLRGRLVSGIQN"
BASE COUNT         3743 a         4589 c         4598 g         3997 t
ORIGIN      
        1 tggctgtggt tttatacagt cattactgcc cgtaatatgc cttcgcacca tgcttacgca
       61 gatagtgttt atccagcagc gtttgctgca tatccggtaa ctgcggcgct aactgacggc
      121 agaatatccc catataagcg acctcttcca gcacgatggc gttatgcacc gcatcttcgg
      181 catttttgcc ccatgcaaat gggccgtgag aatggaccag gacgccgggc atttgtgctg
      241 cgtcgattcc ctgtttttcg aaggtttcta cgatgacgtt accggtttcc cactcatatt
      301 caccgttgat ttctgcgtcg gtcattttgc gggtgcaggg aatggtgccg tagaaatagt
      361 cggcgtgggt ggtaccggtt gccggaattg actggcccgc ctgcgcccag atggtggcgt
      421 ggcgcgagtg tgtgtgcaca atgccgccaa tggacgggaa tgcctgatag agcagccggt
      481 gagttggcgt gtcggaggag ggctttttcg ttccttcaac cacttcaccg gtttcgatgc
      541 tgaccacgac catatcgtca gcggtcatga cgttgtaatc gacgccggaa ggtttgatca
      601 ccaagacgcc gcgctcgcga tcaacggcgc tgacattgcc ccatgtgagc gtgatcaggt
      661 tatgttttgg cagcgccaaa ttggcttcta atacctggcg tttgagatct tctaacatgt
      721 tgactccttc gtgccggatg cgctttgctt atccggccta caaaatcgca gcgtgtaggc
      781 ctgataagac gcgtcagcgt cgcatcaggc ggtgaatgcc ggatgcgctt tgcttatccg
      841 gcctacaaaa tcacagcgtg taggcctgat aagacgcgtc agcgtcgcat caggcgttac
      901 acacctgatg cggctactta gcgacgaaac ccgtaataca cttcgttcca gcgcagcgcg
      961 tctttaaacg ctggcaggcg ggtatcgtta tcaatcaccg tgatttcaat gtcgtgcatc
     1021 tcggcgaact ggcgcatatc gttgaggttc agcgcatggc tgaaaacggt atggtgcgcg
     1081 ccaccagcaa ggatccacgc ttcggaagca gttggcagat ccggttgcgc tttccacagc
     1141 gcattcgcca ccggcagttt cggcagggag tgcggtgttt tcaccgtgtc gatgcagtta
     1201 accagcagac ggtaacgatc gccgagatca atcaggctgg cgacaatcgc tggaccggtt
     1261 tgggtattga agatcaggcg ggcaggatcg tctttaccgc caataccgag gtgctgaacg
     1321 tcgaggatcg gtttctcttc cacagcgatc gacggacaga cttccagcat gtgggagccg
     1381 agcaccaggt cattgccttt ctcgaagtga taggtgtagt cctccataaa ggaggtgccg
     1441 ccctgcagac cggttgacat caccttcatg atgcgaagca gggcggcagt tttccagtcg
     1501 ccttcgcccg caaagccgta accctgctgc atcagacgct gtacggccag accaggaagc
     1561 tgtttcagac cgtgcaaatc ttcaaaggtg gtggtgaacg cgtggaagcc accttgttcc
     1621 aggaaacgct taatccccag ctcaatacgc gccgcttcca gcacgttctg gcgtttttcg
     1681 ccgtggattt gtgtggcagg cgtcatggtg tagcagcttt cgtactcatc gaccagcgcg
     1741 ttaacatcgc catcgctgat ggagttcact acctgcacca gatcgccaac cgcccaggta
     1801 ttgacggaga aaccgaactt gatctgtgcg gcaactttat caccatcggt gaccgccact
     1861 tcacgcatgt tatcgccaaa acggcagact ttcagatgac gggtatcctg tttagagacc
     1921 gcctgatgca tccaggagcc gatacgctca tgggcttgtt tatcctgcca gtgaccggta
     1981 acgacggcat gttgctgacg catacgcgcg ccaatgaagc cgaactcgcg accgccatgt
     2041 gcagtctggt tcaggttcat aaagtccata tcgatgctat cccacggcag cgccgcgtta
     2101 aactgggtgt ggaattgcag caacggtttg ttgagcatag tcaggccgtt gatccacatt
     2161 ttggccgggg agaaagtgtg cagccacact accaggccag cgcaacgatc gtcgtaattc
     2221 gcgtcacggc aaatagcggt gatttcatcc ggcgtggtgc ccagcggttt cagcaccagt
     2281 ttgcagggca gtttcgcttc cgtattcagc gcattaacga cgtgctcggc atgttgggtg
     2341 acctgacgca gggtttccgg gccatacaga tgctggctgc caattacaaa ccacacttca
     2401 taattatcaa aaatcgtcat tgtcgtgtcc ttatagagtc ggaacggcct gggcagcctg
     2461 tgccggggcg gaagttggaa gatagtgttg ttcggcgctc atcgcccatt gctgatagcg
     2521 gcgataaagc tgttcaaagc gttgtgcctg ttcgctgcgc ggttgcaggg ttttctctac
     2581 cgcactggcc attttttgct gggctgaggg gatgtctgcg tgcactttcg cggcgacggc
     2641 agcgaaaatc gccgcaccga gcgcacagca ctggtcagag gcaacaattt gcagcgggcg
     2701 attcagcacg tcgcagcagg cctgcataat gacctggttt ttccgcgcga tgccgcccag
     2761 cgccatcacg ttattgacgg cgatcccctg atcggtaaag cactccatga ttgcgcgtgc
     2821 gccaaaggcg gtggcagcaa tcaatccgcc gaacagcagc ggagcgtcgg tagcgaggtt
     2881 cagatcggta atcacccctt tcaggcgttg gttagcgttt ggcgtgcggc ggccgttaaa
     2941 ccagtcgagc accaccggca ggtgatccag agacggattt ttggcccatg cttcggtcag
     3001 cgccggaagc agttgtttct ggctggcgtt gatttgctct ttcagttccg gatgctgggc
     3061 ggcaagctgt tccagcggcc agccgagtac gcgaccgaac caggcgtaga tatcaccaaa
     3121 cgccgattgg cctgcttcca gaccgataaa tccaggcacc acgctgccat caacctgacc
     3181 gcaaatacct ttcactgccc gctcgccaac gctctgtttg ttggcaatca gaatgtcgca
     3241 ggtggaagta ccgataactt ttaccagagc gttaggctgt gcgcctgcgc caactgcgcc
     3301 catatggcag tcaaacgcgc cgccggaaat caccacgctt tcaggtaggc cgagacgctg
     3361 cgcccattcc gggcataagg tgcccaccgg aatatcggca gtccaggtgt cagtgaatag
     3421 cggggaaggc aaatggcgat tgaggatcgg gtccagctca tcaaagaaac tggctggcgg
     3481 caggccgccc cagctttcat gccatagcga cttatgcccg gcgctgcaac gtccgcgacg
     3541 aatatcctgc gggcgggtgg taccggaaag cagagctggc acccagtcgc acagctcaat
     3601 ccacgatgcg gcagattgcg ccacggcgct gtcctggcga gtcacatgca gtatttttgc
     3661 ccagaaccat tcgctggaat aaataccgcc aatatagcgg gagtagtcaa cgttgcccgg
     3721 cgtgtgacat aagcgggtta tctcttccgc ttcttcaacc gcggtgtggt ctttccacaa
     3781 tacgaacatc gcgttcgggt tttcggcaaa ctccgggcgc agcgccagga cattaccgtc
     3841 cgcgtcaatc ggtgcgggcg tcgagccggt tgtgtcaacg ccaatcccga ccacagctgc
     3901 gcgctgctcg gcgctaagct ctgcaagcac ggttttcagc gccgcttcca ttgactcaat
     3961 gtagtcacgc ggatgatgac ggaactggtt atttggggca tcacaaaatt gccctttctg
     4021 ccaacgggga taccactcta cgctggtggc gatctcttca ccggtggcgc agtccaccgc
     4081 caaagctcgc acagaatcac tgccaaaatc gaggccaatt gcaatcgcca tcgtttcact
     4141 ccatccaaaa aaacgggtat ggagaaacag tagagagttg cgaaaaaaag cgtcaggtag
     4201 gatccgctaa tcttatggat aaaaatgcta tggcatagca aagtgtgacg ccgtgcaaat
     4261 aatcaatgtg gacttttctg ccgtgattat agacactttt gttacgcgtt tttgtcatgg
     4321 ctttggtccc gctttgttac agaatgcttt taataagcgg ggttaccggt tggattagcg
     4381 agaagagcca gtaaaagacg cagtgacggc aatgtctgat gcaatatgga caattggttt
     4441 cttctctgaa tggcgggagt atgaaaagta tggctgaagc gcaaaatgat cccctgctgc
     4501 cgggatactc gtttaacgcc catctggtgg cgggtttaac gccgattgag gccaacggtt
     4561 atctcgattt ttttatcgac cgaccactgg gaatgaaggg ttatattctc aatctcacca
     4621 tacgcggcca gggggtggtg aaaaatcagg gacgagaatt tgtctgccga ccgggtgata
     4681 ttttgctgtt cccgccagga gagattcatc actacggtcg tcatccggag gctcgcgaat
     4741 ggtatcacca gtgggtttac tttcgtccgc gcgcctactg gcatgaatgg cttaactggc
     4801 cgtcaatatt tgccaatacg ggtttctttc gcccggatga agcgcaccag ccgcatttca
     4861 gcgacctgtt tgggcaaatc attaacgccg ggcaagggga agggcgctat tcggagctgc
     4921 tggcgataaa tctgcttgag caattgttac tgcggcgcat ggaagcgatt aacgagtcgc
     4981 tccatccacc gatggataat cgggtacgcg aggcttgtca gtacatcagc gatcacctgg
     5041 cagacagcaa ttttgatatc gccagcgtcg cacagcatgt ttgcctgtcg ccgtcgcgtc
     5101 tgtcgcatct gttccgccag cagttaggaa ttagcgtctt aagctggcgc gaggaccaac
     5161 gcattagtca ggcgaagctg cttttgagca ccacccggat gcctatcgcc accgtcggtc
     5221 gcaatgttgg ttttgacgat caactctatt tctcgcgagt atttaaaaaa tgcaccgggg
     5281 ccagcccgag tgaattccgt gccggttgtg aagaaaaagt gaatgatgta gccgccaagt
     5341 tgtcataatt ggtaacgaat cagataattg acggcttgac ggagtagcat agggtttgca
     5401 gaatccctgc ttcgtccatt tgacaggcac attatgcaag cattgctgga acactttatt
     5461 acccaatcca ccgtgtattc attgatggcg gtggtgttgg tagcctttct ggagtcgctg
     5521 gcgctggtcg gtttgattct acccggtacg gtgctaatgg cgggactggg ggcgctgatt
     5581 ggcagtggcg agttaagttt ctggcacgcc tggctggcag ggatcgtcgg ttgtctgctg
     5641 ggcgactgga tctccttctg gctgggctgg cgttttaaaa agccgttgca tcgctggtca
     5701 tttctgaaga agaacaaagc attactcgat aaaaccgaac atgcgctgca tcagcacagc
     5761 atgttcacca ttctggtcgg tcgctttgtt ggcccgacgc gtccgctggt gccaatggtg
     5821 gcgggaatgc tggatctgcc ggtggcaaaa ttcattacgc cgaatattat cggctgcctg
     5881 ctgtggccac cgttttactt cctgccaggg attctggcgg gtgcggcgat cgatattcca
     5941 gccggaatgc agagcggcga gtttaaatgg ttgctgctgg cgacggcggt atttttgtgg
     6001 gttggtggct ggctgtgctg gcggttatgg cgcagcggta aagcgactga ccgtttgagt
     6061 cattatttgt cgcgcggtcg tttgttgtgg ctggcaccgt taatttctgc cgtcggtgta
     6121 gtggcgctgg tggtgttaat tcgccacccg ttgatgccgg tgtatatcga tattttgcgt
     6181 aaagtggttg gggtttaggg gatagccttg tgcgggttgc ctgatgcgac gcttgccgcg
     6241 tcttatcagg cctacaaaac gcactgtccg taggtcggat aaggcgttca cgccgcatcc
     6301 gacagtgcat actaacccgt aatccccaat attgccgaag cactcgcctt accgctcaac
     6361 aactcatcgg tcataccctg ccaggcgatg cgcccgtcgg cgaccaccac cgagcgcgtg
     6421 gcgatccgcg ccgcatcttc cacactgtgc gacaccatca atagcgtcat tttttgctgt
     6481 tggcagctcg tgctcaccag cgtcaacatc tcctgacgta acgccggatc gagcgcagag
     6541 aacggttcat cgagcaataa aatcggctgt tcgcgcacca gacaacgcgc taacgccacg
     6601 cgctgtcgct gaccgccaga aagctcgccc ggtaagcgcg ccattaaatt atcaatcccc
     6661 atctgacggg caatcgcgtg cattttctcc tgttgtgccg cgttcagctt caatcctgga
     6721 ttaagcccca gcccgatgtt ctgcgcgacc gtcaggtgac tgaacaggtt gttttcctga
     6781 aacagcatcg acaccggacg gcgtgacggc ggcgtcgtgg tgtgatcaac gccgtcgata
     6841 gtcagcgaac cgctggctgg cgtcagaaaa ccggcgatca aattcagcag ggtactttta
     6901 cccgcgccgc ttggcccgag gatcgccacc tgctcgctgc gttccaccgt taagctaaaa
     6961 cgcatcggca aatggtggta aagccaggtg atatcagtca gttttaacat ttcgccccgg
     7021 tagtttttca atcacggtaa acagcagaaa acagagtagc agcagaatta acgcggtgac
     7081 cgcgccgtcc tggctgcgat aggagccaat ttgctggtag agataaaacg gcagggtgcg
     7141 gaaatcatcg ttaccaaaca acgccaccac gccaaaatca ccaatcgata gcacacaagc
     7201 aaaggccagt gcctgcgcca gtgggcgttt aagggcgcgc agctccacca cttttaagcg
     7261 tgaccagcct tcaatgccca gcgactgaca caacatactg taacgcgcgg tgatatcgcg
     7321 catcgggttt tccagcactt tcagcgcata agggatcgcc attaacgcat tggtgaaaat
     7381 cacaatgccg tcagcagatt gtggcaggcc gatagtgttg ttgagcagta aaaagaagcc
     7441 ggtagccagc acaatccccg gcatcgcgag gatcaacatg ccgctcatct ccagcgcctg
     7501 acccgccagc attttctgcc gcgcccgcag ttcgcgactg ctccatagca gcatcatggt
     7561 cagcactacg cataatacgc ctgccgccag cgcaatacgc aacgaggtcc acagcgcctg
     7621 ccacagcacc ggctgcgcca gcacttccgg caactggcga tttatcccat cgacgagcac
     7681 cgccagtaac ggtggcagca acagcaacag cgccagcaca attaacaccg tgtcgcaaat
     7741 gcggctatgc agacgatcgt ccgggtcgcg ccagccttgc agcagcgtag tgccgggcgc
     7801 aatggcctta ctcaatcgct gactcaacag caccagccca aggcaacaca ccatctggat
     7861 cagcgccagc atcgccgcgc gggcaggatc gtagtcgtaa ctcagcgcct gataaatagc
     7921 cagctcgata gtcgtcgctt gcggaccgcc gcccagcgac aacacggtgg cgaagctggc
     7981 gaaacagagc ataaagataa gcgcggcaac cggcgggatt tgtcgccgta accacggcca
     8041 ttcgacgaag cggaaaaaat gccagccgcg catcccaagc tgggcggcaa gctgacgttg
     8101 ttccccggga atgttttcca gtgcctggag taataagcgg ctcgccatcg gcagattaaa
     8161 aaatacatgc gccagcaaaa tgccttgcag gccgtagggc gaaaacgtcc actccagacc
     8221 aagcgattgc cagagcgatg ccagccagcc ctggcgacca tagacgctaa gaatgccgaa
     8281 aacagcaacc agcaccggga ggatcaaggt catggcacac agacgcaaca gcgccagccg
     8341 tcctggaaag cgcctgcggt agagggcgcg ggcaaggaat atcgcgggca cgacagagag
     8401 cagcgccgag agaaacgcct gccagaagga gaagcgcacc acatgccaca ggtagctgtc
     8461 ctgccagatt gccacccagt caccctgcgg cgcgttccac cacagggcga gaaacgcagc
     8521 cagtgcaacc gccaccacca gcgtggcggc gcttattcct ggaattaacc agccgggaat
     8581 taacggctga cggcgcgttg ccattcgcta atccatgcct gacgttgtgc cgccacttcg
     8641 gctggcgtga actccagcgt ggttgccggt ttggtcaatt gttcaaaacc ggcaggcagc
     8701 gtgacgtttg ccaccggata catccagttg ccggttggga tcgcattctg aaaagccgga
     8761 gaaaccataa actggagaaa tttttgcgcc agctccggct gcttgctggc agcggtgcgg
     8821 gcggcgactt ccacctgcag atagtgacct tcgctgaagt ttgcggcggc gtagttatct
     8881 ttcttctctt cgagaatgtg ataagccgga gaggtggtgt aactcagtac cagatcgctt
     8941 tcacctttta aaaacaggcc gtaggcttcg ctccagcctt tggtgaccgt gaccgttttc
     9001 ttcgccagtt tctgccaggc ttgtggggcg tcatcgccat agactttttg catccacaac
     9061 agcaggccca gccccggcgt actggtgcgc ggatcctgat aaatcacccg ccagttttga
     9121 tcgctctcaa caagctcttt caggctttgc ggcggatttt tcagtttgtt cttgtcatag
     9181 acgaaggcga aatagccgta atcaaacggt acgaaagtgt catttttcca gccgccggga
     9241 acgttaacgg catccgctgc cacgccgctt ttggcaaata gtccggtttt actggcggcg
     9301 tctaacaggt tgttatccag ccccagcacc acatcggctt tactgttttt gccttccatc
     9361 cgcagacggt tgagcagcga aacgccatct tccagcgcca ccagtttcag ttcgcagtta
     9421 cagtcggctt caaaagcttt tttgatcttt ggaccaggcc cccagtcggc ggcgaaggaa
     9481 tcgtaggtat aaacagtcag aacgggttta gcgaaaacgg gcgctgtgca cagcaacagc
     9541 aaggggagac atttttttaa cactttgcac ctcaaaaaag agtggcaaag gacttgagaa
     9601 ggagcctcaa atcccttcgc cggcgttatc cggatcaggt tcgacgggta ttttctcagc
     9661 gcacgcgtct gcgtggcacc ccgttgagaa cggcgttagt gtagtgattt tgttatcaac
     9721 cagcaatcat ggatccggcg gcgcaaacca cgctgattta aaatcgaacc agccgagggt
     9781 attcatacgc aggccgcgca tactgcgttg cccctgaatg atcagccagt ggtggataag
     9841 cggcaccatt gctttgctgg cgaccagttg ctggcaccag ttcgccaggt ttatttcgcc
     9901 attgcgccag cgggcggcgt cggcttgcca gtcgatcgga atacagtgtt gtagcagcgg
     9961 cacctcgcac aggtgcgcga atagcgaaaa atccagcggc agcgtaaagt tggcgctgtt
    10021 aagccagata tcgctctcga tctctccttc atgccactga tcgtagctga tctctttgat
    10081 ttccagcgtg acctggtgac ttgccagaat ctgctgcatg atcccggcaa tcaccctatg
    10141 ctcactgtga tcctgataaa aggtcagggt gaggctttcc agaccagccg gtttttcgct
    10201 ctttatggtg cgggtatggt gccaacgggg aagcagccca taggccggaa accacaactg
    10261 ctggtactgt tcctcggcga aataaaccag attggtcggc gaaagcacat agcttatcca
    10321 gtctctgact tgctgattcg ccccgcgatg ggtacggctg tcgaacagta aatagtaaca
    10381 accttcttca aggcggcttt caatctcttt ttcctcgtct tgcggtcctt tcagcatcag
    10441 gcctcccgcg gggtcatcag caatttccgg caagacccag acgttaactt cgtcgattaa
    10501 tgcccgataa ccaaagaagt cgtcgaatgc ctgaattttc agttgattgc tgctgttgcg
    10561 gatcaccgca taaggacccg ttccgatggg atggctggca aagttgctga gggtttccca
    10621 ttcgcgcggc aggatcatcg ccggaacttg ccccagcagt aacggtaacc agcggtccgg
    10681 ttgcgtgaga tggatatcca gcgtccaggg cgtcggcgac acaatgtcag caatatgcga
    10741 atagagcggc agcgtattga ttcgttttaa agaggcgatc acgtcgtcca tttccagttc
    10801 acgaccatga tgaaaatgga ctcctggacg caaaaagaaa cgccagtgaa gcggtaaaat
    10861 ttgctgccag tggtgggcga tgtccgcttc cagttcccca ttttcctcat ttatgcgcgt
    10921 tagcgaactg aagatttgcc gggcgatatg ggtttcggaa cgacgcaatg cactgccggg
    10981 cagcagatta cgcaacggac gatagtagag aacgcgcagg atgtgccgcc cctggcggaa
    11041 gctgcgtccc agatgagaaa ccaacatttg ccgcacagcc gctttgtcgc caaccagttg
    11101 caccagttga tcgatacgat cctgctccag caggtcctct gctcgctgtt gctgaagcgc
    11161 cagcccggta tagaggaatg tcagacgcga gcgtttgccg cgcccgactt ccgcttccca
    11221 cgtcagccag ccgcgatcct gcatggtgtt gagcagggtg cgcatatgac gacgcgagca
    11281 gctcaataac gctgccagtt cgttgagcgt cgtgtcctgc gatttaccct cgcagcattg
    11341 ccacaggcgg atgaactgtt gttgcagacg agcagatggc ataaaagggg aactcctgcg
    11401 caaaagacag caattttatt ttccctatat taagtcaata attcctaacg atgaagcaag
    11461 gaggtgcctc atgcgtcagt tttatcagca ctattttacc gcgacagcga agttgtgctg
    11521 gttgcgttgg ttaagcgtcc cacaacgatt aaccatgctt gaagaactga tgcaatggga
    11581 ggggagtcat tctgactact gatttgcgga catcatgtgt gactgagtat tggtgttaat
    11641 cgccacgcca gcagtgatta tctgctggct tttttctttt tggcgattga gtgccggacg
    11701 gtgtacaaac taacgcataa acgccggttg cttctcttca taagacgcaa ttgcgtcgtc
    11761 gtgctgcaga gtcaggccga tgctgtccag accgttcatc atgcagtggc ggcggaaggc
    11821 atcgatggta aagcgataag ttttctctcc cgctttcacc tcttgcgctt ccaggtctac
    11881 atcaaaatga atccccggat tggcctgcac cagcgcaaac agttcgtcca cttctgcgtc
    11941 gcttaatttc accggcagca gctggttgtt aaagctgttg ccgtagaaga tgtcagcaaa
    12001 actcggtgca atcaccactt taaaaccgta atcggtcaat gcccacggcg cgtgctcacg
    12061 cgaggaaccg cagccgaagt tttctcgtgc cagcaaaatg gaagcgccct gatactgcgg
    12121 gaagttcagc acgaagtccg ggtttggctg ttggcctttt tcatccagaa aacgccagtc
    12181 gttaaacaga tgcgcgccaa aacccgtacg ggtcactttc tgcaaaaact gtttcgggat
    12241 gattgcatcg gtatcgacat tggcggcatc cagcggaacc accaggcctg tgtgtttgat
    12301 aaatttctct gccatggtgt gctccttatt taatgttgcg aatgtcggcg aaatgtccgg
    12361 tcacagcagc agcggcagcc attgccgggc tgaccagatg ggtgcgccct ccacgcccct
    12421 ggcggccttc aaagttacgg ttgctggtgg aggcacaacg ctcacccgga ttcaggcgat
    12481 cgttgttcat cgccagacac attgagcaac ctggcaagcg ccattcaaaa ccggcttcaa
    12541 taaagatttt gtccagacct tccgcttccg cctgggcttt taccgggcca gagccgggaa
    12601 ccaccagtgc ctgcacgcct ggcgcgactt tccgtccttt ggcgatttca gctgccgcgc
    12661 gtaaatcttc aatacgtgag ttagtgcagg aaccgataaa cactttgtcg atagccactt
    12721 ctgtcagcgg aatacccggt ttcagcccca tataggccag cgctttttct gccgacgcgc
    12781 gttcaaccgg atcggcaaac gaagccggat cgggaatgtt gtcgttcacg gaaatcacct
    12841 gacctggatt ggtgccccag gtgacctgcg gtgaaatttc ttctgcctgc aaagtcacaa
    12901 cggtatcgaa agttgcgcct tcgtcggttt gcagagtttt ccagtaggca acggcgtcgt
    12961 cgaaatcttt gcctttcggc gcatgcagac ggcctttgac atagttaaag gtagtttcgt
    13021 ccggtgcaac cagaccggct ttcgcgccca tttcgattgc catattgcac agggtcatac
    13081 gaccttccat gcttaaatca cggattgctt cgccgcaaaa ctccaccaca tgcccggtgc
    13141 cgcctgcgct accagtttta ccgataatcg ccagcacgat atctttcgct gtaatgcccg
    13201 gcgcggcttt gccctggact tcaattttca tggtcttcgc acgaccctgt ttcagggttt
    13261 gcgttgccag tacgtgttca acttcggaag tgccgatacc aaaggctagt gcgccaaacg
    13321 cgccgtgggt ggcggtatgc gagtcgccgc agacaatggt catccccggc aatgtgacgc
    13381 cctgttccgg ccccattacg tggacgatcc cctgatacgg gtgattcagg tcatacagct
    13441 cgacgccaaa ttctttgcag tttttgatca gctcctgcat ctggatgcgc gccatttcac
    13501 cgcaggcatt aatgtctttg gtctgagtag agacgttgtg atccatagtg gcgaaggttt
    13561 tgcctggctg acgtaccggg cgaccgtggg cgcgcagacc atcgaacgcc tgcggtgagg
    13621 tcacttcatg caccaggtgg cggtcgatat ataacagcgg ggtttcgttt tcggcttcgt
    13681 acactacgtg agcgtcgaac aatttttcgt ataacgtttt agccatgatt acaccccttc
    13741 taccacatag cgggcaatga tatcgcccat ttcattggta ctaacggcgg cagcgccacg
    13801 ggctaaatcc ccggtgcgaa tgccttcttc taatgcgcgg ttaatggcgc gttcaatggc
    13861 gcaagccgca tcatctgcat ccaggctgta gcgcagcagc agtgccagcg acagaatttg
    13921 cgcaatcggg ttggcgatgt ttttgcctgc gatatctggt gctgagccgc ctgccggttc
    13981 atacaaacca aaaccttgct cgttcaggct ggcggaaggc aacatcccca tcgagccagt
    14041 gatcattgcg cactcgtcag acagaatgtc gccaaacagg ttggaacaca gcaggacgtc
    14101 aaactgtgat ggatctttaa tcagctgcat ggtggcgttg tcgatgtaca tatgcgccag
    14161 ttcgacatcc gggtattccg tggcgatctc gttgacgatt tcacgccata aaatagagga
    14221 ttgcagcacg ttggctttat cgatcgacgt cactttatgg cgacgtttac gagcagattc
    14281 aaacgcgatg cgggcaatac gctcgatctc aaaacggtga tacacctcgg tatcaaacgc
    14341 tttttcatat tgtccgctac cttcgcggcc ttttggctga ccgaaataga tgccgccggt
    14401 cagttcacgc acacacagga tgtcgaagcc gttagcggca atgtcagcac gcagtggaca
    14461 gaatgcttcc agcccctgat acagttttgc cggacgcagg ttgctgaata atttgaagtg
    14521 cttacgcaga ggcagcagcg cgccgcgttc tggttgctgg tctggtggta aatgttccca
    14581 cttcggaccg cctaccgagc caaacagcac ggcatcggct tgctcacaac cttcaaccgt
    14641 cgcaggcggc agtggttgcc cgtggttatc aatggctgcg ccgcctacat cgtaatggct
    14701 ggtggtgata cgcatcgcaa atcggttgcg cacggcatcc agcactttca gcgcctgagt
    14761 catcacttcc ggaccaatac cgtcccccgg caatacggca atatggtaat tcttcgacat
    14821 cacacggttt ccttgttgtt ttcgttgtgt tgagctttgc gttgcaactc tttttcgact
    14881 tctgcggcac gccagatatt gttcagtacg tgcaccatgg ctttggcgga ggactcgaca
    14941 atatcggtgg ccaggccgac gccgtggaag cggcgaccgt tgtagttggc gacaatatcc
    15001 acctgaccca gcgcatcttt accgtgacct ttggcggtca ggctgtattt caccagttcg
    15061 acgttatagt cagtgatgcg gtttatcgcc tggtagacgg catcgaccgg accgttaccg
    15121 ttggcggctt ctgctttgac ttcttcgccg caggccagtt tgacggcggc agtggcaata
    15181 tcgttagaac ctgactgcac gctgaagtaa tccagacgga aatgctccgg ctcttcttgc
    15241 tgcttaccga tgaaggccag cgcctccaga tcgtaatcaa acacctgacc ttttttgtcc
    15301 gccagcttca ggaaggcatc gtacaaattg tctaaattat attcactttc tttatacccc
    15361 atctcatcca tgcggtgttt tactgccgca cgccccgaac gagaggtcag attcagctgg
    15421 atttggttca aaccaataga ttctggtgtc atgatttcgt agttttcgcg gtttttcagc
    15481 acgccatcct ggtggatacc ggaggagtgt gcgaatgcgc cgctgccaac aatggctttg
    15541 tttgccggga tcggcatatt acaaatctgg ctaactaact ggctggtgcg ccatatctcc
    15601 tggtgattaa tagcggtgtg gacgttgagg atatccttac gaactttgat cgccatgatg
    15661 acttcttcca gcgaacagtt accggcacgc tcgccgatcc cgttcattgc accttccacc
    15721 tggcgtgcac cggcatgtac cgccgccagt gagttgccga ccgccaggcc cagatcgtcg
    15781 tgggtatgta cggaaataat ggctttgtcg atgttgggta cgcgttcata cagcccgctg
    15841 atgattccgg cgaactcaaa tggcatggtg tagcccacgg tgtccggaat gttgatggtg
    15901 gtggcaccgg cattaatcgc cgcttcgacc actcgcgcca gatcggcaat tggggtgcgt
    15961 ccggcatctt cgcaagaaaa ttcaacatca tcggtgtaat tacgggcgcg tttcaccata
    16021 tagatagcgc gttcgatcac ttcgtccagc gtgctgcgca gcttggtggc gatgtgcatt
    16081 ggcgaagtgg caataaaggt atgaatacgg aaggcttcgg cgactttcag ggattcggct
    16141 gccacgtcaa tatctttttc cacgcagcga gctaacgcgc atacacggct atttttaacc
    16201 tggcgagcga tggtctgcac tgattcaaaa tcacccggcg aagagacggg gaaaccgact
    16261 tccatcacgt caacacccat acgctcaagg gccagcgcaa tttgcagttt ttctttcaca
    16321 ctcaagcttg cctgtaacgc ctgttcaccg tcgcgcaatg tggtatcgaa aataatgact
    16381 tgctggctca tggtttgggt ccttgtctct ttttgagcgc ctcgcttcgg gcataaaaaa
    16441 acccgcgcaa tggcgcgggt tttttgtttg actgagtgct gacttaattc tgaatgccgc
    16501 tcaccagtct accgcgcaaa gaagatgcgt ttagtagtag tagaccgata aagcgaacga
    16561 tgtgagtcat taaatcagct ccagatgaat gcgatatgct tttagagtta ctggatacca
    16621 aaacggatgt caaccctgat gcaacaaaaa cgtccagcca gcgtgagttc tgcatccgtc
    16681 aaattagcta attgtgctgc ggtggttaaa acaagcgata ttaatttctg cttaaccacc
    16741 gccgcttttc atcggttgac atatttcaga ataaattttt gcatctaatc gacgagggaa
    16801 agggggataa aatgcacgcg ttacaaaacc tatcctgatg atttgtattg aattatatgt
    16861 attgcgaatt ttttgatatt catttggtga atattattga tcaattaatg ttaagaatta
    16921 aaacatt
//
LOCUS       DAZFLD010000108        16879 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.109, whole genome shotgun sequence.
ACCESSION   DAZFLD010000108 DAZFLD010000000
VERSION     DAZFLD010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16879
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.109"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(281..1519)
                     /locus_tag="ACHKBL_002914"
     CDS             complement(281..1519)
                     /locus_tag="ACHKBL_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000085198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO5819760.1"
                     /translation="MTRAIHRLSDTLLRKLSGSPTTKNTFFNDGGNLSVRHSTNGLLT
                     WYFTYRAGTGRKVPPERLRLGNYPDLSLKAAREKAAQCRAWLAEGKNPRHELNHTVQE
                     ALSPVTVKEALTYWLESYAKEKRTDYESLKSRINTHIISQIGAMPLEKCELRHWLTCF
                     DQLAKRNPVSAGFLLQVCKQALKYCRKRRYAITNVLDDMVVGDVGKKAEISERVLTNK
                     ELGELLRALDEKIYPPYYSALIRLLIVFGCRTTELRRSEVQEWDFKEMLWTVPKEHSK
                     TKVAIFRPIPEAILPFVTKLEEQNRHTGLLLGELKGQSSVSEYGRTAHRRINQAPWTL
                     HDIRHTFTTMLNDLGVEPHVVEQLTAHQLPGIQRVYNHSRYLDAKRHALDLWMERLEL
                     LHSDDEKIVIMSQRNSAQNS"
     gene            complement(1700..1774)
                     /gene="yljB"
                     /locus_tag="ACHKBL_002915"
     CDS             complement(1700..1774)
                     /gene="yljB"
                     /locus_tag="ACHKBL_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YljB"
                     /protein_id="HFO5819761.1"
                     /translation="MNQKFEAVNAIDRNVTDVADANDR"
     gene            1924..2148
                     /gene="cspD"
                     /locus_tag="ACHKBL_002916"
     CDS             1924..2148
                     /gene="cspD"
                     /locus_tag="ACHKBL_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspD"
                     /protein_id="HFO5819762.1"
                     /translation="MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKA
                     GQSVQFDVHQGPKGNHASVIVPVEVEAAVA"
     gene            complement(2221..4167)
                     /gene="macB"
                     /locus_tag="ACHKBL_002917"
     CDS             complement(2221..4167)
                     /gene="macB"
                     /locus_tag="ACHKBL_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990195 - macrolide transmembrane
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding
                     protein/permease MacB"
                     /protein_id="HFO5819763.1"
                     /translation="MTPLLELKDIRRSYPAGDEQVEVLKGISLDIYAGEMVAIVGASG
                     SGKSTLMNILGCLDKATSGTYRVAGQDVATLDADALAQLRREHFGFIFQRYHLLSHLT
                     AEQNVEVPAVYAGLERKQRLLRAQELLQRLGLEDRTEYYPAQLSGGQQQRVSIARALM
                     NGGQVILADEPTGALDSHSGEEVMAILHQLRDRGHTVIIVTHDPQVAAQAERVIEIRD
                     GEIVRNPPAIEKVNVAGGTEPVVNTASGWRQFVSGFNEALTMAWRALAANKMRTLLTM
                     LGIIIGIASVVSIVVVGDAAKQMVLADIRSIGTNTIDVYPGKDFGDDDPQYQQALKYD
                     DLIAIQKQPWVASATPAVSQNLRLRYNNVDVAASANGVSGDYFNVYGMTFSEGNTFNQ
                     EQLNGRAQVVVLDSNTRRQLFPHKADVVGEVILVGNMPARVIGVAEEKQSMFGSSKVL
                     RVWLPYSTMSGRVMGQSWLNSITVRVKEGFDSAEAEQQLTRLLSLRHGKKDFFTWNMD
                     GVLKTVEKTTRTLQLFLTLVAVISLVVGGIGVMNIMLVSVTERTREIGIRMAVGARAS
                     DVLQQFLIEAVLVCLVGGALGITLSLLIAFTLQLFLPGWEIGFSPLALLLAFLCSTVT
                     GILFGWLPARNAARLDPVDALARE"
     gene            complement(4164..5279)
                     /gene="macA"
                     /locus_tag="ACHKBL_002918"
     CDS             complement(4164..5279)
                     /gene="macA"
                     /locus_tag="ACHKBL_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019898 - extrinsic component of membrane
                     [Evidence IEA];
                     GO_component: GO:1990195 - macrolide transmembrane
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1990961 - xenobiotic detoxification by
                     transmembrane export across the plasma membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide transporter subunit MacA"
                     /protein_id="HFO5819764.1"
                     /translation="MKKRKTVKKRYVIALVIIIVGLITLWRILNAPVPTYQTLIVRPG
                     DLQQSVLATGKLDALRKVDVGAQVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIK
                     EVEATLMELRAQRQQAEAELKLARVTYSRQQRLAQTQAVSQQDLDNAATEMAVKQAQI
                     GTIDAQIKRNQASLDTAKTNLDYTRIVAPMAGEVTQITTLQGQTVIAAQQAPNILTLA
                     DMSTMLVKAQVSEADVIHLKPGQKAWFTVLGDPLTRYEGQIKDVLPTPEKINDAIFYY
                     ARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLSALGDPVGDNRYKVKLLRNGETR
                     EREVTIGARNDTDVEIVKGLEAGDEVVIGEAKPGAAQ"
     gene            5430..6386
                     /gene="ybjX"
                     /locus_tag="ACHKBL_002919"
     CDS             5430..6386
                     /gene="ybjX"
                     /locus_tag="ACHKBL_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
                     /protein_id="HFO5819765.1"
                     /translation="MNMSQLTERTFTPSESLSSLSLFLSLARGQCRPGKFWHRRSFRQ
                     KFLLRSLIMPRLSVEWMNELSHWPNLNVLLTRQPRLPVRLHRPYLAANLSRKQLLEAL
                     RYHYALLRGCMSAEEFSLYLNTPGLQLAKLEGKNGEQFTLELTMMISMDKEGDSTILF
                     RNSEGIPLAEITFTLCEYKGKRTMFIGGLQGAKWEIPHQEIQNATKACHGLFPKRLVM
                     EAACLFAQRLQVEQIIAVSNETHIYRSLRYRDKEGKIHADYNAFWESVGGVCDAERHY
                     RLPAQIARKEIAEIASKKRAEYRRRYEMLDAIQPQMATMFRG"
     gene            complement(6383..8041)
                     /gene="ybjD"
                     /locus_tag="ACHKBL_002920"
     CDS             complement(6383..8041)
                     /gene="ybjD"
                     /locus_tag="ACHKBL_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HFO5819766.1"
                     /translation="MILERVEIVGFRGINRLSLMLEQNNVLIGENAWGKSSLLDALTL
                     LLSPESDLYHFERDDFWFPPGDINGREHHLHIILTFRESLPGRHRVRRYRPLEACWTP
                     CTDGYHRIFYRLEGESAEDGSVMTLRSFLDKDGHPIDVEDINDQARHLVRLMPVLRLR
                     DARFMRRIRNGTVPNVPNVEVTARQLDFLARELSSHPQNLSDGQIRQGLSAMVQLLEH
                     YFSEQGAGQARYRLMRRRASNEQRSWRYLDIINRMIDRPGGRSYRVILLGLFATLLQA
                     KGTLRLDKDARPLLLIEDPETRLHPIMLSVAWHLLNLLPLQRIATTNSGELLSLTPVE
                     HVCRLVRESSRVAAWRLGPSGLSTEDSRRISFHIRFNRPSSLFARCWLLVEGETETWV
                     INELARQCGHHFDAEGIKVIEFAQSGLKPLVKFARRMGIEWHVLVDGDEAGKKYAATV
                     RSLLNNDREAEREHLTALPALDMEHFMYRQGFSDVFHRVAQIPENVPMNLRKIISKAI
                     HRSSKPDLAIEVAMEAGRRGVDSVPTLLKKMFSRVLWLARGRAD"
     gene            8467..9162
                     /gene="aqpZ"
                     /locus_tag="ACHKBL_002921"
     CDS             8467..9162
                     /gene="aqpZ"
                     /locus_tag="ACHKBL_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015250 - water channel activity [Evidence
                     IEA];
                     GO_process: GO:0006833 - water transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HFO5819767.1"
                     /translation="MFRKLAAECFGTFWLVFGGCGSAVLAAGFPELGIGFAGVALAFG
                     LTVLTMAFAVGHISGGHFNPAVTIGLWAGGRFPAKEVVGYVIAQVVGGIVAAALLYLI
                     ASGKTGFDAAASGFASNGYGEHSPGGYSMLSALVVELVLSAGFLLVIHGATDKFAPAG
                     FAPIAIGLALTLIHLISIPVTNTSVNPARSTAAAIFQGGWALEQLWFFWVVPIVGGII
                     GGLIYRTLLEKRN"
     gene            9619..10518
                     /gene="lysO"
                     /locus_tag="ACHKBL_002922"
     CDS             9619..10518
                     /gene="lysO"
                     /locus_tag="ACHKBL_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine exporter LysO"
                     /protein_id="HFO5819768.1"
                     /translation="MFSGLLIILVPLIVGYLIPLRQKAALRVINQLLSWMVYLILFFM
                     GISLAFLDNLASNLLAILHYSAVSITVILLCNIAALMWLERGLPWRNHHHQEKLPSRI
                     AMALESLKLCGVVVIGFAIGLSGLAFLQHATEASEYTLILLLFLVGIQLRNNGMTLKQ
                     IVLNRRGMIVAVVVVASSLIGGLINAFILDLPINTALAMASGFGWYSLSGILLTESFG
                     PVIGSAAFFNDLARELIAIMLIPGLIRRSRSTALGLCGATSMDFTLPVLQRTGGLDMV
                     PAAIVHGFILSLLVPILIAFFSA"
     gene            10662..12314
                     /gene="hcp"
                     /locus_tag="ACHKBL_002923"
                     /gene_synonym="priS"
     CDS             10662..12314
                     /gene="hcp"
                     /locus_tag="ACHKBL_002923"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HFO5819769.1"
                     /translation="MFCVQCEQTIRTPAGNGCSYAQGMCGKTAETSDLQDLLIAALQG
                     LSAWAVKAREYGIINHDVDSFAPRAFFSTLTNVNFDSPRIVGYAREAIALREALKAQC
                     LAVDANARVDNPMADLQLVSDDLGELQRQAAEFTPNKDKAAIGENILGLRLLCLYGLK
                     GAAAYMEHAHVLGQYDNDIYAQYHKIMAWLGTWPADMNALLECSMEIGQMNFKVMSIL
                     DAGETGKYGHPTPTQVNVKATAGKCILISGHDLKDLYNLLEQTEGTGVNVYTHGEMLP
                     AHGYPELRKFKHLVGNYGSGWQNQQVEFARFPGPIVMTSNCIIDPTVGAYDDRIWTRS
                     IVGWPGVRHLDGEDFSAVIAQAQQMAGFPYSEIPHLITVGFGRQTLLGAADTLIDLVS
                     REKLRHIFLLGGCDGARGERHYFTDFATSVPDDCLILTLACGKYRFNKLEFGDIEGLP
                     RLVDAGQCNDAYSAIILAVTLAEKLGCGVNDLPLSLVLSWFEQKAIVILLTLLSLGVK
                     NIVTGPTAPGFLTPDLLAVLNEKFGLRSITTVEEDMKQLLSA"
     gene            12326..13294
                     /gene="hcr"
                     /locus_tag="ACHKBL_002924"
     CDS             12326..13294
                     /gene="hcr"
                     /locus_tag="ACHKBL_002924"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidoreductase"
                     /protein_id="HFO5819770.1"
                     /translation="MTMPTNQCPWRMQVHHITQETPDVWTISLICHDYYPYRAGQYAL
                     VSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGVGSQWLTRDVKRGDYLWLSDAMG
                     EFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNRPQADVQVIYNVRTPQDVIFADEW
                     RNYPVTLVAENNVTEGFIAGRLTRELLAGVPDLASRTVMTCGPAPYMDWVEQEVKALG
                     VTRFFKEKFFTPVAEAATSGLKFTKLQPAREFYAPVGTTLLEALESNNVPVVAACRAG
                     VCGCCKTKVVSGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLVLA"
     gene            13427..15145
                     /gene="poxB"
                     /locus_tag="ACHKBL_002925"
     CDS             13427..15145
                     /gene="poxB"
                     /locus_tag="ACHKBL_002925"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0052737 - pyruvate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HFO5819771.1"
                     /translation="MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEW
                     MSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLHLINGLFDCHRNHVPVLAIAAHI
                     PSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
                     GDVALKPAPEGATTHWYHAPQPVVTPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKE
                     LVEFAGKIKAPIVHALRGKEHVEYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQF
                     PYRAFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALLPLVEEKADRKFLDK
                     ALEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLK
                     MNGKRRLLGSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLMGDFLSVVQMK
                     LPVKIIVFNNSVLGFVAMEMKAGGYLTDGTELHDTNFARIAEACGITGIRVEKASEVD
                     EALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGDEVIELA
                     KTNWLR"
     gene            15182..16183
                     /gene="ltaE"
                     /locus_tag="ACHKBL_002926"
     CDS             15182..16183
                     /gene="ltaE"
                     /locus_tag="ACHKBL_002926"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HFO5819772.1"
                     /translation="MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAA
                     ELSGKEAAIFLPTGTQANLVALLSHCERGEEYIVGQAAHNYLFEAGGAAVLGSIQPQP
                     IDAAADGTLPLDKVAMKIKPDDIHFARTKLLSLENTHNGKVLPREYLKEAWEFTRERN
                     LALHVDGARIFNAVVAYGCELKEITQYCDSFTICLSKGLGTPVGSLLVGNRDYIKRAI
                     RWRKMTGGGMRQSGILAAAGMYALKNNVARLQEDHDNAAWMAEQLREAGADVMRQDTN
                     MLFVRVGEENAAALGEYMKARNVLINASPIVRLVTHLDVSREQLAEVAAHWRAFLAR"
     gene            16194..>16879
                     /locus_tag="ACHKBL_002927"
     CDS             16194..>16879
                     /locus_tag="ACHKBL_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HFO5819773.1"
                     /translation="MPQRILVLGASGYIGQHLVRTLSQQGHQILAAARHVDRLAKLQL
                     ANVSCHKVDLSWPDNLPALLQDIDTVYFLVHSMGEGGDFIAQERQVALNVRDALREVP
                     VKQLIFLSSLQAPAHEQSDHLRARQATADILREAGVPVTELRAGIIVGAGSAAFEVMR
                     DMVYNLPVLTPPRWVRSRTTPIALENLLHYLVALLDHPASEHRIFEAAGPEVLSYQQQ
                     FEHFMAVSGKR"
BASE COUNT         3948 a         4431 c         4312 g         4188 t
ORIGIN      
        1 aaggttcgta gaagctcgtt agtgtttaaa atcgtgtcac gagtttttaa gtgacgcaac
       61 aaaaaatgtc gtcacttggc atgacagctg aattatctca ttaaaaacaa atagataaac
      121 catcaagcat tgaaacaatc aagaaataga gaataaggat tcaggagagt gaatttcaat
      181 tttttcgatg attgacaaat aaaaaaacga cgaactttgg tcgaacttcg acgatctttt
      241 taagacggct attttcccga agaaaaacag ccgtaatttg tcaagaattt tgagcgctat
      301 ttcgctgact catgataacg attttttcat cgtcgctatg cagaagttcg agtctttcca
      361 tccagagatc gagcgcgtgg cgttttgcat ctagatagcg ggagtggtta tacacccttt
      421 gtattcctgg taactggtgc gcggtaagct gctccacaac gtgcggttcc acgcctaaat
      481 cgttcagcat cgttgtaaac gtgtgccgga tgtcgtgcaa cgtccagggg gcttgattaa
      541 tgcgtctgtg tgccgttctt ccgtactctg atacagatga ttgccctttc agttctccga
      601 gcaataagcc tgtgtgcctg ttctgttcct ccagcttcgt gacgaacggc aagatcgctt
      661 ccggtattgg cctgaatatt gctaccttcg ttttgctgtg ttctttcggc acggtccaga
      721 gcatttcttt aaaatcccat tcctggacct cagagcgtct tagctcagtg gtacggcatc
      781 cgaagacaat caggaggcga atcagggcgc tgtagtacgg tgggtaaatt ttttcatcca
      841 gggcgcggag taattcccca agctccttgt ttgttagtac gcgctcgctt atttctgctt
      901 ttttcccaac atcacctaca accatatcat cgagaacgtt ggtaattgcg tagcgtcgtt
      961 tgcggcagta cttaagcgcc tgcttgcata cctgtaacaa aaatcctgct gataccggat
     1021 tacgcttagc aagctgatca aaacaggtca gccagtggcg tagctcgcat ttttccagcg
     1081 gcatagcacc aatctgactg attatgtgtg tattgatccg gctcttcaga gattcgtagt
     1141 ctgtgcgctt ttccttcgcg tacgattcaa gccagtaagt gagcgcttcc ttaaccgtaa
     1201 caggtgataa cgcttcctgt acggtgtgat taagctcatg gcgtggattt ttcccctcag
     1261 caagccaggc gcgacactgc gccgcttttt ccctggctgc tttcaggctc aaatctggat
     1321 aatttcccag cctcagacgt tcagggggta ccttcctgcc tgttccggcc ctgtaagtga
     1381 aataccaggt taacaggcca ttggttgaat gccttacgct aaggttgcca ccatcattaa
     1441 aaaaggtgtt ttttgtggtt ggtgatccgc ttaattttcg taaaagagtg tcgctaagtc
     1501 tatgaattgc cctggtcatg tttcgaattt ccggtcatca aaattaacta caccattgac
     1561 tacaccgagc atcgtacaag ggcgaactgt gacgaacaag ggcgaacaag aaaaacgaaa
     1621 ttatccttta taatcataga aataaatgag atacccacac catgacgaac acccacgaac
     1681 actaactgat aaataactat catctatcgt tggcatcagc gacatctgtc acattcctgt
     1741 caatagcgtt aactgcttca aatttttgat tcatacctgc cccctggccc ctgtcaaatg
     1801 cttgacggct cgccctaatt ctctaaattg tatttctaga gttggcgagg ttttgaacag
     1861 ccccctctct gaccccggtt tattccatct tacttgtata agatttgcga aggatgtcga
     1921 agcatggaaa agggtactgt taagtggttc aacaatgcca aagggtttgg tttcatctgc
     1981 cctgaaggcg gcggcgaaga tattttcgct cattattcca ccattcagat ggatggttac
     2041 agaacgctaa aagctggaca atccgttcag tttgatgtcc accaggggcc aaaaggcaat
     2101 cacgccagtg ttattgtgcc cgtcgaagta gaagcggcag tcgcatagct cttctgtctc
     2161 attgtgtaca tcctaaaggc aaaatgccag cccgatcggc tggcattttt atctcaaaaa
     2221 ttactctcgt gccagagcat ctactggatc cagtcgtgcc gcatttcgtg cgggtaacca
     2281 gccaaataaa atcccggtga ccgtcgagca gagaaacgcc agcagcagcg ccaacggtga
     2341 aaaaccaatc tcccagccgg gtaagaaaag ctgtaaggtg aaagcaatta acagtgacag
     2401 tgttattccc aacgcgccac cgaccaggca aaccagtacg gcttcaatca ggaactgttg
     2461 caaaacatcg cttgctcgcg cacctacagc catgcgaatg ccaatttccc gcgtccgctc
     2521 ggtcactgac accagcataa tattcattac accaataccg cccaccacca gcgaaatcac
     2581 cgccaccagc gtcagaaaca gttgtaaagt acgtgtggtc ttttcaacgg ttttcaagac
     2641 gccgtccatg ttccaggtaa agaaatcctt cttaccgtgg cgcagtgaaa gtaaacgcgt
     2701 gagttgctgt tccgcctcag cgctgtcaaa gccttctttc accctgacag taatggagtt
     2761 cagccacgac tggcccataa ctcgcccgga catcgtgctg taaggtagcc agacacgcag
     2821 cactttactg ctaccaaaca tcgactgttt ttcttccgcc acaccaatga ctctggcggg
     2881 catattgccg accagaatca cctcgccaac cacatctgct ttatggggga aaagctggcg
     2941 gcgagtatta ctgtcgagca ccaccacctg cgcacgaccg ttcagctgct cctgattaaa
     3001 ggtgttccct tcactgaagg tcatgccata gacattaaaa taatcgccgc tcacgccatt
     3061 ggcactggca gccacatcaa cattgttata acgcaggcgc aggttttgcg agacggcagg
     3121 tgtggctgag gcgacccacg gttgtttttg gatggcgatt aagtcgtcgt acttcagcgc
     3181 ctgctgatat tgcggatcgt catcgccaaa atctttcccg ggatagacat caatagtatt
     3241 cgtaccaata gaacgaatat ccgccagcac catttgtttg gcggcgtcac ccaccacgac
     3301 aatggaaacc accgacgcaa taccgataat aatccccagc atggtcagta aagtacgcat
     3361 tttattcgct gccagcgccc gccatgccat cgtcagcgcc tcgttaaaac cgctgacaaa
     3421 ctgccgccag ccagacgccg tgttgacaac aggttccgtc ccgccagcaa cattcacttt
     3481 ttcaatggcg ggaggattgc gcacaatttc accgtcgcga atttcgatca cccgctcggc
     3541 ctgagcagcg acctgcggat cgtgggtaac gataatcacc gtatgcccgc gatcgcgcag
     3601 ctgatgcagg atcgccatca cctcttcgcc agaatggctg tccagtgcgc cggtcggttc
     3661 atcggcaaga attacctgac cgccgttcat caatgcccgc gctatgctga cgcgctgttg
     3721 ctgaccaccc gaaagctgtg ccgggtaata ctctgtacgg tcttccagcc ccagtcgttg
     3781 cagcaactcc tgggcgcgaa gcagccgctg tttccgctca agaccggcat agacggcggg
     3841 tacttcaacg ttctgctcgg cggttaaatg cgaaagcaaa tggtaacgct ggaaaataaa
     3901 gccgaaatgc tcgcggcgta gttgcgccag cgcatcggcg tccagcgtgg caacatcctg
     3961 accggctacg cgataggtgc cgctggtggc cttatccagg cagccgagaa tattcatcag
     4021 ggtcgattta ccggaacccg aagcgccgac aatcgccacc atctcaccag cataaatatc
     4081 gaggctgatg cccttcagca cctcaacctg ctcatcaccg gcaggatagc tgcgacgaat
     4141 atcctttaat tcgagcaagg gtgtcattgc gcagctcctg gtttggcctc accaatcacc
     4201 acttcatcgc ccgcttccag ccctttgaca atctcaacat cggtatcgtt acgtgcgcca
     4261 atcgtcactt cacgctcgcg tgtttcacca ttacgcaaca atttgacttt ataacgatta
     4321 tcgccaaccg gatcgcctaa cgccgacaga gggatcgtca gcacattttt cacatcggtg
     4381 agctgaatat gcacttgcgc ggtcatatcc agccgcagta aaccattggg gttgggtact
     4441 tcaaaacggg cgtaatagaa aatagcgtcg ttaatctttt ccggcgtcgg tagcacatcc
     4501 ttgatttgcc cctcgtagcg cgtcagtgga tcgccaagca ccgtaaacca ggctttttgc
     4561 cccggcttca ggtggattac atccgcttca gaaacctgcg cttttaccag catggtgctc
     4621 atatctgcca gcgtcagaat gttcggtgct tgttgtgcgg caatcaccgt ctggccttgc
     4681 agagtggtga tttgcgtgac ttccccggcc atcggggcaa cgatgcgggt gtaatcgaga
     4741 ttggttttag ccgtatcgag agaggcctga ttgcgcttga tttgcgcgtc aatggtgcca
     4801 atttgcgcct gtttcacagc catctccgtc gcggcgttat cgagatcctg ctgtgaaacc
     4861 gcttgcgttt gtgcaagacg ttgctgacgg gaatacgtca cccgcgccag tttcagctcc
     4921 gcttccgcct gctgccgctg cgcgcgtagc tccatcagcg ttgcttccac ctccttaatc
     4981 tggttttcag cctgttcagg atcaataacc cctaaaagct ggtctttttt tactttatcg
     5041 ccaatcgcca ccgacagcgt tttcaactga ccgctgacct gcgcgcccac atcaacctta
     5101 cgcagcgcgt ccagctttcc ggtcgccagc acgctttgct gtaaatcacc ggggcgcaca
     5161 atcagcgtct gataagtcgg cacgggtgcg ttaagaattc tccataacgt aatcagcccg
     5221 acgatgatta tcaccagcgc aataacgtaa cgcttcttca cggttttccg ctttttcata
     5281 aatattctga atactccata tgccaatccg ggtcattaac ttcaacgaaa tatcaacaaa
     5341 accctaaaat ttacattagt aatcacacta ttgatgtttg gttaagatac gcaatggtga
     5401 aatcgacgtc atgtataacc attgatttca tgaatatgtc gcagctaact gaacggacct
     5461 ttacgccatc tgaatctctc agcagcctgt cactttttct tagtctggca cgtggacagt
     5521 gtcggccggg taaattctgg catcgccgta gttttcgcca gaaatttttg ctgcgctcgt
     5581 tgattatgcc gcgtttaagc gttgagtgga tgaacgaact ttcccactgg cccaatctca
     5641 atgtgttgtt aacgcgccag ccgcgactgc ctgtgcgtct gcatcgccct taccttgcgg
     5701 cgaatcttag ccgtaagcaa ttgctggagg cgttacgtta ccattatgcg ttactccgcg
     5761 ggtgtatgtc ggcggaagaa ttcagcttat atttgaatac ccccgggctg caactggcta
     5821 aactggaagg caaaaacggc gagcagttca cgcttgagct gaccatgatg atctcaatgg
     5881 ataaagaagg tgacagcaca atcctgttcc gcaacagcga aggtattcct ctggcagaga
     5941 tcacgtttac cctgtgtgaa tataagggga aaagaacaat gtttattggc ggactgcaag
     6001 gcgcaaaatg ggaaattcca catcaggaaa tccagaatgc gacgaaagcc tgccacgggc
     6061 tatttcccaa acgccttgtg atggaagcgg cctgtctgtt tgcccaacgt ttgcaggtag
     6121 agcagattat tgccgtcagc aatgaaacgc atatttaccg cagcctgcgt tatcgtgata
     6181 aagaaggcaa gatccatgcc gattacaacg cgttctggga gtcggttggc ggcgtatgtg
     6241 atgctgaacg ccattaccgc cttccggcac agatagcacg aaaagagatt gccgaaatcg
     6301 ccagtaaaaa acgggctgaa taccgtcggc gctatgagat gctcgacgct attcagccac
     6361 aaatggccac gatgtttcgc ggttaatccg cgcgaccgcg cgccagccac agcacgcgtg
     6421 agaacatttt tttcagcagc gtcggtacgg agtccacacc acgacgtccg gcctccattg
     6481 ccacttcaat ggcaagatcg ggtttggaag agcgatggat cgctttcgag ataattttgc
     6541 gcagattcat cggtacattt tccgggattt gcgccacgcg gtggaacaca tcggaaaatc
     6601 cctggcgata cataaaatgt tccatatcca gcgccggtaa cgccgttaaa tgttctcgtt
     6661 cggcttcccg atcgttattc aacaggctgc gtaccgtagc ggcatatttc ttccctgctt
     6721 catcgccatc gaccagtaca tgccattcaa tccccattcg gcgggcaaat ttaaccagtg
     6781 gctttagccc ggactgggca aactcaatga ccttgattcc ttcggcatca aaatgatgtc
     6841 cgcactgacg cgccagttca ttgataaccc aggtttccgt ttccccttcc accagcagcc
     6901 agcagcgagc aaacagcgat gacggacggt taaaacgaat gtgaaaggat atgcgtcggc
     6961 tatcttcggt actcaagcca ctcggcccca gacgccaggc ggcaacgcgc gaggactcac
     7021 gtaccagtcg gcaaacatgc tctaccggcg ttaacgaaag taactcaccc gagttggtgg
     7081 tggcaatgcg ctgcaagggc agaagattca acagatgcca ggcaactgaa agcataatgg
     7141 ggtgtaaacg ggtttctgga tcttcgatca gcaacaatgg acgggcgtct ttatccagtc
     7201 gcaatgtgcc ttttgcctgc aataaagtag caaacaggcc gagcaaaata acccgatacg
     7261 agcgccctcc aggtcggtca atcatccggt tgatgatatc cagatagcgc cagcttcgtt
     7321 gctcattgct ggctcgccgc cgcattaaac gatatcgcgc ctgtccagct ccctgctcag
     7381 aaaaataatg ctcaagaagt tgcaccattg cggaaagtcc ctgacgaatc tgcccatcag
     7441 agagattttg cggatgtgag gataattcac gggcgaggaa atcgagctgg cgcgcggtga
     7501 cttccacatt ggggacattt ggcaccgtgc cgttacgaat acggcgcata aaacgggcat
     7561 cacgcaagcg cagcaccggc attaaacgca ccagatgacg agcctgatcg ttaatatcct
     7621 caacatcaat cggatgtccg tctttatcga gaaaactgcg cagtgtcatc acgctgccgt
     7681 cttccgcact ctccccttcc agacgataaa aaatacggtg ataaccatcg gtgcatggcg
     7741 tccagcacgc ttccagcggc cgatagcggc gaacccgatg tcggcctggc agcgattcgc
     7801 ggaaggtcaa aataatatgc agatgatgtt ctcgcccgtt gatatctccc ggcgggaacc
     7861 agaaatcgtc gcgctcaaaa tggtagagat ctgattctgg cgatagcagc agggttaagg
     7921 cgtccagcaa gctggattta ccccacgcgt tctccccaat caggacgttg ttttgttcca
     7981 gcatcaacga caaacggttg ataccgcgaa aacccacaat ttcaacgcgc tcaagaatca
     8041 tatcccctcc agaaatatgt gacttatcct ttacgctggc agtataacgg gaaaggccat
     8101 gctaatacgt gatgacatca catttaagca tggcaaatgt ctgaaaaatg agcgaataat
     8161 cgcgttatac tccctcttgc tgataagtct gcaaaaacaa caaaatcaaa taattagcat
     8221 gataaattat tttttattac gcctaaagga ttaaatcttt cgcttgcact acactgtcaa
     8281 tcagacaaat gactatatgg ggcaacccgc tgttgcctta aatcaaattt actggattta
     8341 tttatctggt gaatgactcg ccttcgtttt taaaatagcc gcgcattatt tccgtcgtca
     8401 cttttttacg acgaccctat aaaacgacca tatttttcac agggtcaatt tttaattgag
     8461 gtggatatgt tcagaaaatt agcagctgaa tgttttggta ctttctggct tgtttttggt
     8521 ggctgtggta gtgccgtact ggccgcaggc ttcccggaat taggcattgg ttttgccggc
     8581 gtggcgctgg cattcggtct gaccgttctg acgatggcct ttgctgttgg tcatatttct
     8641 ggtggtcatt ttaacccggc ggtcactatt ggtttatggg ctggcggacg tttcccggca
     8701 aaagaagtcg ttggctacgt aattgcccag gttgtcggcg gtattgttgc ggcggcgctg
     8761 ctgtatttaa ttgccagtgg taaaacaggt tttgacgcgg cagccagcgg ttttgcttct
     8821 aacggttatg gcgagcattc gccaggcggt tattccatgc tttccgcgct ggtagttgaa
     8881 ctggtattga gtgcaggttt cctgctggtg atccacggcg caaccgacaa attcgctcct
     8941 gctggttttg cgccgatcgc tattggtctg gccttaaccc tgattcactt aatcagtatt
     9001 ccggtgacta acacttctgt taacccggcg cgcagcaccg cggctgctat cttccagggc
     9061 ggctgggcgt tagaacaact gtggttcttc tgggtggtgc caattgtcgg cggtattatc
     9121 ggtggtctga tttaccggac cctgctggaa aagcgtaatt aattagcatt ggcattttat
     9181 ctgccctggt tgttttatct tcgacgttac aaaccgcacg ggtgttggtg gcgtcaaaat
     9241 cgctgaagcg ttccccgaag gccggggcag cccacatgga tgtgggctga gggcgcgtct
     9301 tacaggacgt tacctcgcgc ccgacccggt agccgaaggg gataagctga aggcaccgcg
     9361 cagcggcgat tttgttcgcc agagcccggg ggtgcagggg gcggcggcga ctggccgccc
     9421 cctgcgcgct ccctgcgcca gtggctttat atggcattgc acatgaaagg agcgcaacaa
     9481 tgctaaacac tcatatagaa ttaaaaatct ccctctgatg cataacattt ccttttaatt
     9541 gcaactttac tcgtccttcc gctttcagta gtgtgtcatc gcgcatttcg acttcatctt
     9601 tgcaaggact gggttttcat gttttctggg ctgttaatca ttctggttcc cctgattgtg
     9661 ggttacctca ttccgcttcg ccaaaaagct gcattaagag ttattaatca gctattaagc
     9721 tggatggttt accttattct cttttttatg ggtatcagtc tggcgtttct cgataacctc
     9781 gccagtaacc tgttggcgat tctgcattat tctgccgtca gtattaccgt tattttactg
     9841 tgtaatattg ccgccctgat gtggctggag cgcggcctgc catggcgcaa ccaccatcat
     9901 caagaaaaac tcccgtcgcg tattgcgatg gcgctggagt cgctaaaact gtgcggcgta
     9961 gtagtgattg gttttgccat tggtctgagt ggactggctt tcttacaaca cgcgaccgaa
    10021 gccagtgaat acaccttaat tctgctactt ttcctcgtcg gtattcagtt gcgcaataat
    10081 ggcatgacct taaagcagat tgtccttaat cgccggggaa tgattgtcgc cgtggtggtg
    10141 gttgccagtt cattaattgg tggtttaatt aacgccttta ttcttgatct tcccatcaat
    10201 accgcgctgg caatggcctc cggtttcggc tggtattctc tttccggtat tttattgacc
    10261 gaatcttttg gtccggtaat cgggagcgcg gcgtttttta atgatctggc ccgtgagctg
    10321 attgctatta tgttaatccc tgggctgatt cgccgtagcc gctctactgc actgggctta
    10381 tgcggtgcga cgtcgatgga tttcaccctg cccgttcttc aacgtacagg cgggctggat
    10441 atggtcccgg cggcgattgt tcacggtttt attcttagtc tgttagtgcc gattctcatc
    10501 gcctttttct ctgcataata cctctctggc ggtagatctc tgccgccaaa attgcgctaa
    10561 atcaatctcc cttaaagttg cattaaaaat ctcttttatc cccgcgttaa gcgtcttaac
    10621 cttaaacatg tatattaaat ataactttaa aaggtgtgat catgttttgt gtgcaatgtg
    10681 aacaaactat ccgtactccg gcaggaaacg gctgctcata cgcgcagggg atgtgtggta
    10741 aaacggcaga aacctctgac cttcaggatt tactcatcgc ggcgctgcaa gggctttcgg
    10801 cctgggcagt gaaagcacgt gaatacggca ttatcaacca cgatgtagac agctttgcgc
    10861 cacgtgcctt tttctcaacc ctgaccaacg ttaacttcga ttctccgcgt attgtcggct
    10921 acgctcgtga agcgattgcc ctgcgcgagg cgctgaaagc acaatgcctg gctgtagatg
    10981 ccaacgcccg cgtcgataac ccgatggctg acctgcaact ggtgagcgac gatctcggcg
    11041 agctgcaacg tcaggcagca gaatttaccc ctaacaaaga taaagcggcg attggcgaaa
    11101 acattctcgg cctgcgtctg ctgtgcctgt atggcctgaa aggtgcggcg gcctatatgg
    11161 aacacgcgca tgttctcggt caatacgaca acgatattta tgcccagtac cataaaatca
    11221 tggcgtggct ggggacctgg cctgccgata tgaacgcgct tcttgagtgt tcaatggaaa
    11281 tcggccagat gaacttcaaa gtgatgagca ttctggacgc aggcgaaacc ggtaaatacg
    11341 gtcacccgac gccaactcag gtcaatgtca aagcgacggc gggtaaatgc attctgattt
    11401 ccggtcacga tctcaaagat ctctacaacc tgctggaaca gaccgaaggc acgggcgtta
    11461 atgtttacac ccacggcgaa atgctgcctg cgcatggcta cccggagttg cgtaaattca
    11521 agcatctggt cggtaactac ggcagcggct ggcagaatca gcaagtggag ttcgctcgtt
    11581 tcccaggccc catcgtgatg acctcgaact gcatcatcga cccaaccgta ggcgcttatg
    11641 acgatcgtat ctggacccgc agcatcgttg gctggccggg tgtgcgtcat ctggatggcg
    11701 aagatttctc tgcagtcatt gcccaggcgc aacagatggc gggcttcccg tacagcgaaa
    11761 ttccgcacct gatcaccgtg ggctttggtc gccagacact gcttggcgca gccgatacgc
    11821 tgattgatct ggtgagccgt gaaaaactgc gtcatatctt cctgcttggc ggctgtgacg
    11881 gcgcacgcgg cgagcgtcac tatttcaccg atttcgccac cagcgtgccg gatgactgcc
    11941 tgatcctgac cctcgcttgc ggtaaatatc gctttaataa acttgagttt ggcgatatcg
    12001 aaggtctgcc gcgtctggta gatgctggtc aatgtaacga tgcttactca gcaattattc
    12061 tggcggtaac gctggcagag aaactgggct gcggagtcaa cgatctgccg ctgtcgctgg
    12121 tgctctcctg gtttgaacag aaagcgattg tcattctgct gacgctgctt tctctgggtg
    12181 tgaaaaatat cgtcaccggc ccgactgcac ctggtttcct gacaccagac ctgctggcgg
    12241 tgctgaacga gaaattcggc ctgcgttcta tcaccactgt tgaagaagac atgaagcaac
    12301 tgttgagcgc gtaaggagtt tgtcgatgac gatgccaacg aatcaatgcc cgtggcggat
    12361 gcaggttcat cacattacgc aagaaacgcc ggatgtgtgg acgatttccc tgatttgcca
    12421 cgattactac ccatatcgcg ccgggcaata tgcactggtc agcgtgcgta actcagcgga
    12481 aacgctgcgt gcttacacca tttcctccac gccaggcgtg agtgaataca tcaccctgac
    12541 tgtgcggcgg attgatgacg gtgtcggctc ccagtggctg acgcgcgatg taaaacgcgg
    12601 tgattatctc tggctttcgg acgcgatggg ggaatttacc tgcgatgata aagcagaaga
    12661 taaattcctg ttgctggcgg caggctgcgg cgttacgccg attatgtcga tgcgtcgctg
    12721 gctggcgaag aaccgtccac aggccgatgt gcaggtgatc tacaacgtgc gtacgccgca
    12781 ggatgtgatt ttcgccgatg agtggcgtaa ctatccggta acgctggtgg cggaaaataa
    12841 cgttaccgaa ggctttatcg ctggtcgtct cactcgcgaa ctgctggcag gtgtccctga
    12901 tttagcctca cgtaccgtga tgacctgtgg ccctgctcca tatatggatt gggtagagca
    12961 ggaagtgaaa gcgctcggcg tgacgcgttt ctttaaagag aaattcttca ccccagtagc
    13021 ggaagcggcg accagcggtc tgaaattcac caaactgcaa ccggcacgag aattttacgc
    13081 cccggttggc accacgctac tggaggcgct ggaaagcaat aacgttccgg ttgtcgccgc
    13141 ctgccgcgca ggtgtttgcg gctgctgtaa gacgaaagtg gtttccggtg aatatacggt
    13201 gagcagcaca atgacgctga ccgacgccga aatcgctgaa ggttatgtac tggcctgctc
    13261 ctgccatccg cagggggatt tggttctcgc ataatcgcct gatgcccgat gatattcctt
    13321 tcatcgggct atttaaccgt tagtgcctcc tttttctccc atcccttccc cctccgtcag
    13381 atgaactaaa cttgttaccg ttatcacatt caggagatgg agaaccatga aacaaacggt
    13441 tgcagcttat atcgccaaaa cactcgaatc ggcaggcgtg aaacgcatct ggggagtcac
    13501 gggcgactct ctgaacggtc ttagtgacag tcttaatcgc atgggcacca tcgagtggat
    13561 gtccacccgg catgaagaag tggcggcatt tgccgctggc gctgaagcac aacttagcgg
    13621 agaactggcg gtctgtgccg gatcgtgcgg tcccggcaac ctgcacttaa tcaacggcct
    13681 gttcgattgc caccgcaatc acgttccggt actggcgatt gccgctcata ttccctccag
    13741 cgaaattggc agcggctatt tccaggaaac ccacccacaa gagctattcc gcgaatgtag
    13801 tcactattgc gagctggttt ccagcccaga gcagatccca caagtgctgg caattgctat
    13861 gcgcaaagcg gtgcttaacc gtggcgtttc cgttgttgtg ttaccgggcg acgtggcgtt
    13921 aaaacctgcg ccagaagggg caactaccca ctggtatcat gcgccacagc cggtagtaac
    13981 accggaagaa gaagagttac gcaaactggc gcagctgctg cgttattcca gcaatatcgc
    14041 cctgatgtgt ggcagcggct gtgcgggggc gcataaagag ttagttgagt ttgccgggaa
    14101 aattaaagcg cctatagtcc atgccctgcg cggtaaagaa catgtcgaat acgataatcc
    14161 atatgatgtt ggaatgacgg gattaatcgg cttttcgtca ggtttccata ccatgatgaa
    14221 cgccgacacg ttagtgctgc tcggtactca gtttccctac cgtgcgttct acccgaccga
    14281 tgccaaaatt attcagattg atatcaaccc agccagcatc ggcgctcaca gcaaggtgga
    14341 tatggcactg gtcggcgata tcaagtcgac tctgcgtgca ttgctgccat tggtggaaga
    14401 aaaagccgat cgcaagtttc tggataaagc gctggaagat taccgcgacg cccgcaaagg
    14461 gctggacgat ttagctaaac cgagcgagaa agccattcac ccgcaatatc tggcgcagca
    14521 aattagtcat tttgccgccg atgacgctat tttcacctgt gacgttggta cgccaacggt
    14581 gtgggcggca cgttatctaa aaatgaacgg caagcgtcgc ctgttaggtt cgtttaacca
    14641 cggttcgatg gctaacgcca tgccgcaggc gctgggtgcg caggcaaccg agccggaacg
    14701 tcaggtggtc gccatgtgcg gcgatggcgg ttttagcatg ttgatgggcg atttcctctc
    14761 agtagtgcag atgaaactgc cggtgaaaat tatcgtcttt aataacagcg tgctgggctt
    14821 tgtggcgatg gagatgaaag ccggaggcta cctgacagac ggtactgagc tgcacgacac
    14881 caactttgcc cgcattgccg aagcgtgcgg cattacgggt attcgtgtag aaaaagcctc
    14941 tgaagtcgat gaagccctgc aacgcgcctt ctccatcgac ggtccggtgt tggtggatgt
    15001 ggtggtcgcc aaagaagagt tagccattcc accgcagatc aaactcgaac aggccaaagg
    15061 tttcagcctg tatatgctgc gcgcaattat cagcggacgc ggcgatgaag tgatcgagct
    15121 ggcgaaaacg aactggctaa ggtaaaaagg gtggcatttc ccgtcataat aaggacatgc
    15181 catgattgat ttacgcagtg ataccgttac ccgaccgagc cgcgccatgc tcgaagcgat
    15241 gatggccgcc ccggttgggg acgacgttta cggagacgac cctaccgtta atgctctgca
    15301 ggactatgca gcagagcttt ccggtaaaga agccgccatt tttctgccta ccggcactca
    15361 ggccaacctg gtcgctctgc tcagtcactg cgaacgcggc gaagagtata tcgtcggtca
    15421 ggccgcgcat aactatctgt ttgaagccgg tggcgcagca gtgctgggca gtattcagcc
    15481 gcaacccatc gacgcggctg ccgacggcac gctacctctg gataaagtgg cgatgaagat
    15541 caaacccgac gatatccatt tcgcccgcac caaattactc agtctggaaa acacccacaa
    15601 cggcaaagtg ttgccgcggg aatacctgaa agaagcatgg gaatttactc gcgagcgcaa
    15661 tctggcgctg catgttgacg gtgcgcgcat ctttaatgcc gtggtggctt acggctgcga
    15721 actgaaagag atcacgcaat attgtgattc gttcaccatt tgcctgtcga aaggtcttgg
    15781 gacgccggtc ggttcattac tcgtcggtaa tcgtgattac attaaacgtg ccattcgctg
    15841 gcggaaaatg acaggtggcg ggatgcgtca gtccggcatt ctggctgccg ccgggatgta
    15901 tgccctgaaa aataacgtcg cacgtttgca ggaagaccac gataacgccg cctggatggc
    15961 ggagcagttg cgtgaagcag gcgcggatgt gatgcgtcag gacaccaata tgctgtttgt
    16021 tcgcgtcggg gaagaaaatg ctgccgcgtt aggcgaatac atgaaagcga gaaacgtgct
    16081 gattaacgcc tcgccgattg tccgcctggt gacccatctt gacgtctcgc gtgaacaact
    16141 ggcggaagtt gccgcccact ggcgtgcgtt cctggcgcgt taaggagaga gacgtgccgc
    16201 aacgcatttt agttctcggt gccagtggct acattggtca gcatctggtg cgaactctca
    16261 gccagcaagg gcatcagatc ctggcggcgg cacgtcatgt cgacaggctt gcaaagctgc
    16321 aactggcaaa cgtcagttgc cataaagtcg atctcagctg gccggataac cttccggccc
    16381 tgttgcagga tatcgatacg gtctattttc tggtgcacag catgggcgaa ggcggcgatt
    16441 ttatcgctca ggagcgccag gtggctctca acgtccgcga tgcgctacgc gaagtccctg
    16501 ttaagcaatt aatcttcctc agttcgttgc aggccccggc acatgagcag tctgaccatc
    16561 tgcgcgcccg tcaggctacg gcggatattc ttcgtgaagc gggtgtcccg gtgaccgaac
    16621 tacgtgccgg aataatcgtt ggtgcaggtt cagcggcgtt cgaagtcatg cgcgatatgg
    16681 tctacaacct gccggtgtta acgccgccac gctgggttcg ttcacgcacc acgcccatcg
    16741 cgctggaaaa cttgctgcac tatctggtgg cgctgttaga tcatccggcc agcgaacatc
    16801 gcatcttcga agccgccgga ccagaagtgc tcagttatca gcaacagttt gaacatttta
    16861 tggcggtgag cggtaagcg
//
LOCUS       DAZFLD010000109        16418 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.110, whole genome shotgun sequence.
ACCESSION   DAZFLD010000109 DAZFLD010000000
VERSION     DAZFLD010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16418
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.110"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..182
                     /locus_tag="ACHKBL_002928"
     CDS             <1..182
                     /locus_tag="ACHKBL_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2170 family protein"
                     /protein_id="HFO5819774.1"
                     /translation="NQKMMPLSSVGISSVQHEEYYIVFGALSLKSSLEDILLEITSLV
                     DNALDLAEITEEYSH"
     gene            201..899
                     /locus_tag="ACHKBL_002929"
     CDS             201..899
                     /locus_tag="ACHKBL_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HFO5819775.1"
                     /translation="MGILKSLFTLGKSFISQAEESIEETQGVRMLEQHIRDAKAELDK
                     AGKSRVDLLARVKLSHDKLKDLRERKASLEARALEALSKNVNPSLINEVAEEIARLEN
                     LITAEEQVLSNLEVSRDGVEKAVTATAQRIAQFEQQMEVVKATEAMQRAQQAVTTSTV
                     GASSSVSTAAESLKRLQTRQAERQARLDAAAQLEKVADGRDLDEKLAEAGIGGSNKSS
                     AQDVLARLQRQQGE"
     gene            949..1608
                     /locus_tag="ACHKBL_002930"
     CDS             949..1608
                     /locus_tag="ACHKBL_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjfK family protein"
                     /protein_id="HFO5819776.1"
                     /translation="MSGFFQRLFGKDNKPAIARGPLGLHLNSGFTLDTLAFRLLEDEL
                     LIALPGEEFTVAAVSRIDLGGGSQIFRYYTSGDEFLQINTTGGEDIDDIDDIKLFVYE
                     ESYGISKESHWREAINAKAMGAMTLNWQEKRWQRFFNSEEPGNIEPVYMLEKVENQNH
                     AKWEVHNFTMGYQRQVTEDTYEYLLLNGEESFNDLGEPEWLFLRALGVDIPLTSLHII
                     G"
     gene            1626..2024
                     /locus_tag="ACHKBL_002931"
     CDS             1626..2024
                     /locus_tag="ACHKBL_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HFO5819777.1"
                     /translation="MHILDSLLAFSAYFFIGVAMVIIFLFIYSKITPHNEWQLIKNNN
                     TAAALAFSGTLLGYVIPLSSAAINAVSIPDYFAWGGIALVIQLLVFAGVRLYMPALSE
                     KIINHNTAAGMFMGTAALAGGIFNAACMTW"
     gene            2034..2672
                     /locus_tag="ACHKBL_002932"
     CDS             2034..2672
                     /locus_tag="ACHKBL_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 domain-containing protein"
                     /protein_id="HFO5819778.1"
                     /translation="MARKRKSRNNKNIGHGAISRIGRPHNPFEPRRNRYAQKYLTLAL
                     MGGAAFFVLKGCGDSGDVDNDGDGTFYATVQDCIDDGNNADICARGWNNAKAAFYADV
                     PKNMTQQNCQSKYENCYYDNVEQSWIPVVSGFLLSRVIRKDRDEPFVYNSGGSSFASR
                     PVWRNTSGDYSWRSGSGKKESYSSGGFTTKKASTVSRGGYGRSSSARGHWGG"
     gene            2675..3838
                     /locus_tag="ACHKBL_002933"
     CDS             2675..3838
                     /locus_tag="ACHKBL_002933"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HFO5819779.1"
                     /translation="MLRHNVPVRRDLDQIAADNGFDFHIIDNEIYWDESRAYRFTLRQ
                     IEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAIPPLYWDVIAESWRARDPSLYGRM
                     DFAWCGNAPVKLLEYNADTPTSLYESAYFQWLWLEDARRSGVIPRDADQYNAIQERLI
                     SRFSELYSREPFYFCCCQDTDEDRSTVLYLQDCAEQAGQESRFIYIEDLGLGVGGVLT
                     DLDDNVIQRAFKLYPLEWMMRDDNGPLLRKRREQWVEPLWKSILSNKGLMPLLWRFFP
                     GHPNLLASWFDGEKPQIAAGESYVRKPIYSREGGNVTIFDGQNNVVDHADGDYADEPM
                     IYQAFQPLPRFGDSYTLIGSWIVDDEACGMGIREDNTLITKDTSRFVPHYIAG"
     gene            3922..5547
                     /locus_tag="ACHKBL_002934"
     CDS             3922..5547
                     /locus_tag="ACHKBL_002934"
                     /EC_number="1.3.8.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418608.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HFO5819780.1"
                     /translation="MHWQTHTVFNQPIPLNNSNLYLSDGALCEAVTREGAGWDSDFLA
                     SIGQQLGTAESLELGRLANVNPPELLRYDAQGRRLDDVRFHPAWHLLMQALCTNRVHN
                     LAWEEDARSGAFVARAARFMLHAQVEAGSLCPITMTFAATPLLLQMLPAPFQDWTTPL
                     LSDRYDSHLLPGGQKRGLLIGMGMTEKQGGSDVMSNTTRAERMEDGSYRLVGHKWFFS
                     VPQSDAHLVLAQTTGGLSCFFVPRFLPDGQRNAIRLERLKDKLGNRSNASCEVEFQDA
                     IGWLLGQEGEGIRLILKMGGMTRFDCALGSHAMMRRAFSLAIYHAHQRHVFGNPLIQQ
                     PLMRHVLSRMALQLEGQTALLFRLARAWDRRADAKEALWARLFTPAAKFVICKRGMPF
                     VAEAMEVLGGIGYCEESELPRLYREMPVNSIWEGSGNIMCLDVLRVLNKQAGVYDLLS
                     EAFVEVKGQDRYFDRAVRRLQQQLRKPAEELGREITHQLFLLGCGAQMLKYASPPMAQ
                     AWCQVMLDTRGGVRLSEQIQNDLLLRATGGVCL"
     gene            complement(5655..5930)
                     /gene="yjfN"
                     /locus_tag="ACHKBL_002935"
     CDS             complement(5655..5930)
                     /gene="yjfN"
                     /locus_tag="ACHKBL_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protease activator YjfN"
                     /protein_id="HFO5819781.1"
                     /translation="MKQFLASSSLQLVTYPASATAQSAEFASADCVTGLNEIGQISVS
                     NISGDPQDVERIVALKADEQGASWYRIITMYEDQQPDNWRVQAILYA"
     gene            complement(6079..6408)
                     /gene="bsmA"
                     /locus_tag="ACHKBL_002936"
     CDS             complement(6079..6408)
                     /gene="bsmA"
                     /locus_tag="ACHKBL_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm peroxide resistance protein BsmA"
                     /protein_id="HFO5819782.1"
                     /translation="MVSRKRNSVIYRFASLLLVLMLSACSALQGTPQPAPPVTDHPQE
                     IRRDQTQGLQRIGSVSTMVRGSPDDALAEIRAKAVAAKADYYVVVMVDETIVTGQWYS
                     QAILYRK"
     gene            6590..7339
                     /gene="yjfP"
                     /locus_tag="ACHKBL_002937"
     CDS             6590..7339
                     /gene="yjfP"
                     /locus_tag="ACHKBL_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFO5819783.1"
                     /translation="MIEIESRELADIPVLHAYPVGQKDTPLPCAIFYHGFTSSSLVYS
                     YFAVALAQAGLRVIMPDAPDHGSRFSGDAARRLNQFWQILLQSMQEFTTLRAAIAEEN
                     WLRDDRLAVGGASMGAMTALGITARHSTVKCTASMMGSGYFTSLARSLFPPLIPETAA
                     QQNEFNNIVAPLAEWEATNHLEQLADRPLLLWHGLDDDVVPADESLRLQQALSETGRD
                     KLLTCSWQPGVRHRITPEALDAAVTFFRQHL"
     gene            complement(7336..8091)
                     /gene="ulaR"
                     /locus_tag="ACHKBL_002938"
     CDS             complement(7336..8091)
                     /gene="ulaR"
                     /locus_tag="ACHKBL_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HFO5819784.1"
                     /translation="MTEAQRHQILLEMLAQLGFVTVEKVVERLGISPATARRDINKLD
                     ESGKLKKVRNGAEAITQQRPRWTPMNLHQAQNHDEKVRIAKAASQLVNPGESVVINCG
                     STAFLLGREMCGKPVQIITNYLPLANYLIDQEHDSVIIMGGQYNKSQSITLSPQGSEN
                     SLYAGHWMFTSGKGLTAEGLYKTDMLTAMAEQKMLSVVGKLVVLVDSSKIGERAGMLF
                     SRADQIDMLITGKNANPEILQQLEAQGVSILRV"
     gene            complement(8199..9263)
                     /gene="ulaG"
                     /locus_tag="ACHKBL_002939"
     CDS             complement(8199..9263)
                     /gene="ulaG"
                     /locus_tag="ACHKBL_002939"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035460 - L-ascorbate 6-phosphate
                     lactonase activity [Evidence IEA];
                     GO_process: GO:0019854 - L-ascorbic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HFO5819785.1"
                     /translation="MSKVKSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLG
                     CTGIWLKSEGGTNVCVDFWCGTGKQSHGNPLMKQGHQMQRMAGVKKLQPNLRTTPFVL
                     DPFAIRQIDAVLATHDHNDHIDVNVAAAVMQNCADDVPFIGPKTCVDLWIGWGVPKER
                     CIVVKPGDVVKVKDIEIHALDAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPG
                     GSLYHSGDSHYSNYYAKHGNEHQIDVALGSYGENPRGITDKMTSADILRMGEALNAKV
                     VIPFHHDIWSNFQADPQEIRVLWEMKKDRLKYGFKPFIWQVGGKFTWPLDKDNFEYHY
                     PRGFDDCFTIEPDLPFKSFL"
     gene            9618..11015
                     /gene="ulaA"
                     /locus_tag="ACHKBL_002940"
     CDS             9618..11015
                     /gene="ulaA"
                     /locus_tag="ACHKBL_002940"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFO5819786.1"
                     /translation="MEILYNIFTVFFNQVMTNAPLLLGIVTCLGYILLRKSVSVIIKG
                     TIKTIIGFMLLQAGSGILTSTFKPVVAKMSEVYGINGAISDTYASMMATIDRMGDAYS
                     WVGYAVLLALALNICYVLLRRITGIRTIMLTGHIMFQQAGLIAVTLFIFGYSMWTTII
                     CTAILVSLYWGITSNMMYKPTQEVTDGCGFSIGHQQQFASWIAYKFAPFLGKKEESVE
                     DLKLPGWLNIFHDNIVSTAIVMTIFFGAILLSFGIDTVQAMAGKVNWTVYILQTGFSF
                     AVAIFIITQGVRMFVAELSEAFNGISQRLIPGAVLAIDCAAIYSFAPNAVVWGFMWGT
                     IGQLIAVGILVACGSSILIIPGFIPMFFSNATIGVFANHFGGWRAALKICLVMGMIEI
                     FGCVWAVKLTGMSAWMGMADWSILAPPMMQGFFSIGIAFMAVIIVIALAYMFFAGRAL
                     RAEEDAEKQLAEQSA"
     gene            11031..11336
                     /gene="ulaB"
                     /locus_tag="ACHKBL_002941"
     CDS             11031..11336
                     /gene="ulaB"
                     /locus_tag="ACHKBL_002941"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HFO5819787.1"
                     /translation="MTVRILAVCGNGQGSSMIMKMKVDQFLTQSNIDHTVNSCAVGEY
                     KSELSGADIIIASTHIAGEITVTGNKYVVGVRNMLSPADFGPKLLEVIKEHFPQDVK"
     gene            11346..11810
                     /gene="ulaC"
                     /locus_tag="ACHKBL_002942"
     CDS             11346..11810
                     /gene="ulaC"
                     /locus_tag="ACHKBL_002942"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIA"
                     /protein_id="HFO5819788.1"
                     /translation="MKLRDSLAENKSIRLQAEAETWQDAVKIGVDLLVAADVVEPRYY
                     QAILDAVEQHGPYFVLAPGLAMPHGRPEEGVKKTGFALVTLKKPLEFNHEDNDPVDIL
                     ITMAAVDANTHQEVGIMQIVNLFEDEENFDRLRACRTEQEVLDLIDRTNAAA"
     gene            11824..12474
                     /gene="ulaD"
                     /locus_tag="ACHKBL_002943"
     CDS             11824..12474
                     /gene="ulaD"
                     /locus_tag="ACHKBL_002943"
                     /EC_number="4.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HFO5819789.1"
                     /translation="MSLPMLQVALDNQTMDSAYETTRLIAEEVDIIEVGTILCVGEGV
                     RAVRDLKALYPHKIVLADAKIADAGKILSRMCFEANADWVTVICCADINTAKGALDVA
                     KEFNGDVQIELTGYWTWEQAQQWRDAGIGQVVYHRSRDAQAAGVAWGEADITAIKRLS
                     DMGFKVTVTGGLALEDLPLFKGIPIHVFIAGRSIRDAASPVEAARQFKRSIAELWG"
     gene            12484..13338
                     /gene="ulaE"
                     /locus_tag="ACHKBL_002944"
     CDS             12484..13338
                     /gene="ulaE"
                     /locus_tag="ACHKBL_002944"
                     /EC_number="5.1.3.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase UlaE"
                     /protein_id="HFO5819790.1"
                     /translation="MLSKQIPLGIYEKALPAGECWLERLQLAKTLGFDFVEISVDETD
                     DRLSRLDWSREQRLALVNAIVETGVRVPSMCLSAHRRFPLGSEDDAVRAQGLEIMRKA
                     IQFAQDVGIRVIQLAGYDVYYQEANNETRRRFRDGLKESVEMASRAQVTLAMEIMDYP
                     LMNSISKALGYAHYLNNPWFQLYPDIGNLSAWDNDVQMELQAGIGHIVAVHVKDTKPG
                     VFKNVPFGEGVVDFERCFETLKQSGYCGPYLIEMWSETAEDPAAEVAKARDWVKARMA
                     KAGMVEAA"
     gene            13338..14024
                     /gene="ulaF"
                     /locus_tag="ACHKBL_002945"
     CDS             13338..14024
                     /gene="ulaF"
                     /locus_tag="ACHKBL_002945"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710063.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase UlaF"
                     /protein_id="HFO5819791.1"
                     /translation="MQKLKQQVFEANMDLPRYGLVTFTWGNVSAIDRERGLVVIKPSG
                     VAYETMKADDMVVVDMSGKVVEGEYRPSSDTATHLELYRRYPSLGGIVHTHSTHATAW
                     AQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGDAEPLHTP
                     GIVVYQHGPFAWGKDAHDAVHNAVVMEEVAKMAWIARGINPQLNHIDSYLMNKHFMRK
                     HGPNAYYGQK"
     gene            complement(14153..14428)
                     /gene="yjfY"
                     /locus_tag="ACHKBL_002946"
     CDS             complement(14153..14428)
                     /gene="yjfY"
                     /locus_tag="ACHKBL_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YjfY"
                     /protein_id="HFO5819792.1"
                     /translation="MFSRVLALLAVLLLSANTWAAIEINNHQARNMDDVQSLGVIYIN
                     HNFATESEARQALNEETDAQGATYYHVILMREPGSNGNMHASADIYR"
     gene            14756..15151
                     /gene="rpsF"
                     /locus_tag="ACHKBL_002947"
     CDS             14756..15151
                     /gene="rpsF"
                     /locus_tag="ACHKBL_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HFO5819793.1"
                     /translation="MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGR
                     RQLAYPINKLHKAHYVLMNVEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPM
                     VKAKDERRERRDDFANETADDAEAGDSEE"
     gene            15158..15472
                     /gene="priB"
                     /locus_tag="ACHKBL_002948"
     CDS             15158..15472
                     /gene="priB"
                     /locus_tag="ACHKBL_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030894 - replisome [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HFO5819794.1"
                     /translation="MTNRLVLSGTVCRTPLRKVSPSGIPHCQFVLEHRSVQEEAGFHR
                     QAWCQMPVIVSGHENQAITHSITVGSRITVQGFISCHKAKNGLSKMVLHAEQIELIDS
                     GD"
     gene            15477..15704
                     /gene="rpsR"
                     /locus_tag="ACHKBL_002949"
     CDS             15477..15704
                     /gene="rpsR"
                     /locus_tag="ACHKBL_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HFO5819795.1"
                     /translation="MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLSLLPYTDRHQ"
     gene            15746..16195
                     /gene="rplI"
                     /locus_tag="ACHKBL_002950"
     CDS             15746..16195
                     /gene="rplI"
                     /locus_tag="ACHKBL_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HFO5819796.1"
                     /translation="MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNI
                     EFFEARRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADA
                     VTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE"
BASE COUNT         3876 a         3969 c         4440 g         4133 t
ORIGIN      
        1 gaaatcagaa gatgatgccg ttgtcatcgg tagggatctc cagcgtgcaa catgaagagt
       61 attacattgt tttcggtgcg ttatcgctta aatcttctct tgaagatatc ctgcttgaga
      121 taacctcgct ggtagataac gcattggatc tggctgaaat tacagaagaa tattcacact
      181 aattacaagg acgggtattt atgggaattt taaaaagttt atttacgctg gggaaatcgt
      241 ttatctccca ggcggaagaa tccattgaag aaacccaggg cgtgcgcatg ctggaacagc
      301 atattcgtga cgctaaagct gaactcgata aagccggaaa atctcgcgtt gatctgctgg
      361 cgcgggtgaa attaagtcac gataagctga aagatttacg tgagcgcaaa gccagtctgg
      421 aagctcgtgc gctggaagcg ttaagcaaga acgttaatcc gtcgttgatt aacgaagttg
      481 ctgaagaaat cgcgcgcctt gagaatctca ttaccgctga agagcaagtg ttgtcgaatc
      541 tggaagtctc tcgtgatggc gtggaaaaag cagttacagc gacagcgcag cgtatcgctc
      601 agtttgagca gcaaatggaa gtcgttaaag ctactgaagc catgcagcgt gcacaacagg
      661 ctgtaacgac ctctactgtt ggcgcatctt ccagcgtttc gacagcggca gaatccttaa
      721 aacgcctgca aacgcgtcag gccgaacgtc aggctcgcct ggatgctgcc gcacagttgg
      781 aaaaagtcgc agacggtcgc gatcttgatg aaaagctggc ggaagccgga attggcggta
      841 gcaataaaag tagcgcccag gatgtattag caagactgca acgccaacag ggcgagtaat
      901 tttttgccag cctcactgca aggctggcaa ctattttaag gataaaatat gtctggtttt
      961 ttccagcgtc tgtttggcaa ggataataag cctgctatcg ctcgcggtcc tctgggactt
     1021 catctcaata gtggctttac gctcgacacg ttagcgtttc gtttgctgga agatgaattg
     1081 ctgattgcgc tgccgggtga ggaatttacg gtagccgctg tcagccgcat cgatctgggc
     1141 ggcggtagcc agattttccg ctactacact tcaggcgatg agtttctgca aatcaatacc
     1201 actggcggcg aagatattga tgacattgat gatatcaagc tctttgtcta tgaagagagc
     1261 tacggtatca gtaaagagag tcactggcgc gaggcgatca atgccaaagc gatgggggca
     1321 atgaccttaa actggcagga aaaacgctgg cagcgatttt ttaatagcga agaaccggga
     1381 aatatcgaac ccgtctacat gctggaaaaa gtagaaaatc aaaaccatgc taaatgggag
     1441 gtccataatt ttaccatggg ctatcaacgg caagtgaccg aagatactta cgaatatctg
     1501 ctgttaaatg gtgaggaatc ttttaatgat ctcggcgagc cggagtggtt atttttgcgc
     1561 gcgctggggg tcgatatccc gctgacatca cttcatatta ttggttaatt acaaggacgt
     1621 ttacgatgca tatactggat tcacttcttg cctttagcgc ttattttttt attggtgtgg
     1681 ctatggtgat tatttttctg tttatctact ccaaaattac accgcacaac gaatggcagt
     1741 taatcaaaaa taataacacc gcggcggcac tggcgttcag cggaacattg ttggggtacg
     1801 tgatcccctt atccagtgcg gcaatcaatg cggtgagtat tccagactat ttcgcatggg
     1861 gcgggattgc tctggtgatt caattactcg tttttgctgg cgtcaggctt tatatgcccg
     1921 cattaagcga aaaaattatt aatcacaata ccgcagcagg aatgtttatg ggaaccgccg
     1981 cgctggctgg cggtattttt aacgcagctt gtatgacatg gtaatggacg atcatggcca
     2041 gaaaacgcaa atccagaaat aacaaaaata tcggacatgg ggcaatcagt cgcattggta
     2101 gaccgcataa tccttttgag ccgcgccgca atcgctacgc acaaaaatac ttaacgttgg
     2161 cgctaatggg cggtgccgct tttttcgtat tgaaaggttg tggcgatagt ggcgatgtcg
     2221 ataacgacgg cgacggaacg ttttacgcga ctgtgcagga ttgtattgat gacggtaata
     2281 atgcagacat ttgcgcgcgt ggctggaaca acgccaaagc ggcgttttat gccgatgttc
     2341 cgaagaatat gactcagcag aattgtcagt ctaagtacga aaattgctac tacgacaatg
     2401 ttgaacagag ttggatcccg gtcgtttctg gatttttgtt aagccgggtt attcgtaaag
     2461 atcgcgatga gccgttcgtc tataacagcg gcggatcctc atttgcttcg cgccccgtct
     2521 ggcgcaacac ttctggtgat tactcctggc gctccggttc tggcaaaaaa gagtcttact
     2581 cttcgggcgg ctttaccacc aaaaaagcgt caaccgtttc tcgcggcggc tatggtcgtt
     2641 cttccagcgc ccgtgggcat tggggaggct aatcatgctg agacacaacg ttcctgtgcg
     2701 gcgggatctg gaccagatcg ccgctgacaa cggtttcgac tttcatatca tcgacaatga
     2761 aatctattgg gacgagagtc gagcttaccg ttttactctg cgccagattg aagagcagat
     2821 cgaaaaaccg actgcggaac tgcatcagat gtgccttgag gtggtggatc gcgcggttaa
     2881 agacgaagag atcctgacgc aactggcgat cccgccgttg tactgggatg tgatcgccga
     2941 aagctggcgc gcacgcgatc cttcgttgta tggccgtatg gattttgcct ggtgtggtaa
     3001 tgcaccggtt aagttgctgg agtacaacgc cgatacgcca acttcattgt acgagtcggc
     3061 ttatttccag tggttgtggc tggaagatgc ccggcgcagc ggcgttattc cgcgcgatgc
     3121 cgatcagtac aatgccattc aggagcgcct gatttcacgc tttagcgagc tttacagtcg
     3181 ggaaccgttt tatttttgct gctgtcagga caccgacgaa gacaggagta ccgtgctgta
     3241 cttgcaggac tgcgccgagc aggcagggca ggagtcgcgg tttatctaca ttgaagatct
     3301 cggtttgggc gtcggcggtg tgctgaccga tcttgatgat aatgtcatcc agcgtgcatt
     3361 taagctgtat ccgctggagt ggatgatgcg tgacgataac ggtccgctgc tgcgcaagcg
     3421 tcgcgagcaa tgggtggagc cgttatggaa aagtatcttg agtaataaag ggctaatgcc
     3481 gctgctttgg cgcttcttcc ctggtcatcc taaccttctt gcatcctggt tcgatggtga
     3541 aaaaccgcag attgccgctg gcgaaagcta tgtgcgtaaa ccgatttact cgcgcgaagg
     3601 cggaaacgtc accattttcg acggtcagaa taacgttgtt gaccacgctg atggtgatta
     3661 cgccgatgaa ccgatgatct accaggcgtt tcaacctctg ccgcgatttg gcgatagcta
     3721 cacactcatc ggtagctgga ttgtcgatga tgaagcgtgc ggaatgggga tccgtgaaga
     3781 taacacgttg atcaccaaag acacctcacg tttcgttccg cattacattg ctggataaga
     3841 atgttttagc aatctctttc tgtcatgaat ccatggcagt gaccatacta atggtgactg
     3901 ccattgatgg agggagacac agtgcactgg caaactcaca ccgtttttaa tcaacctata
     3961 ccattaaata acagcaactt atacctgtct gatggcgcgc tctgcgaagc ggtaacgcgt
     4021 gaaggtgctg gctgggatag cgattttctc gccagtattg gtcagcagtt agggacggct
     4081 gaatcccttg aactggggcg gctggcgaat gtgaatccgc ctgaattatt gcgctacgat
     4141 gcccaggggc ggcgtctgga cgatgtgcgt tttcaccccg cctggcacct gctgatgcag
     4201 gcgctatgta ccaatcgggt gcacaatctt gcctgggaag aagacgctcg ctccggcgca
     4261 tttgtggcgc gcgcggcgcg ttttatgttg cacgcacagg ttgaggcagg gtcgttatgt
     4321 ccgataacga tgacctttgc cgccacgcca ttgctgttac agatgttacc cgcgccgttt
     4381 caggactgga ccacgccgct gttgagcgat cgctacgatt ctcacttatt gccaggtggg
     4441 caaaaacgcg gtttgttgat tggcatggga atgacggaaa aacagggcgg ttccgatgtc
     4501 atgagcaaca ccacccgcgc agagcgcatg gaagatggct cttatcggct ggtggggcat
     4561 aaatggtttt tctcggtgcc gcaaagcgat gcgcatctgg tgctggcgca gactacgggc
     4621 ggtttgtcct gcttttttgt gccgcgcttt ttgcctgacg ggcaacgcaa cgcgattcgc
     4681 ctcgagcggc tgaaagataa gctgggtaat cgctctaacg ccagctgcga agtggagttt
     4741 caggatgcca ttggctggtt gttggggcag gaaggggaag gaattcgtct gatcctgaaa
     4801 atgggtggga tgacgcgttt tgattgcgcc ctgggtagcc atgccatgat gcgccgtgca
     4861 ttttcgctag cgatttatca tgcacatcaa cgccatgttt ttggtaatcc attgatccaa
     4921 cagcccctta tgcgtcatgt cttaagtcgc atggcacttc agcttgaagg gcaaacggcg
     4981 ttgctgtttc gtcttgctcg agcgtgggac cggcgtgccg atgccaaaga agccctgtgg
     5041 gcgcgtttat ttacgcctgc ggcgaaattt gtgatctgca aacgaggtat gccgtttgtg
     5101 gccgaagcga tggaggtgct gggcggcatt ggttattgcg aggagagcga gctgccgcgg
     5161 ctttaccggg agatgccggt aaacagtatt tgggaaggtt ccggtaatat tatgtgcctg
     5221 gatgtactgc gcgtgctcaa taagcaagcg ggtgtatacg acttattgtc ggaagcattt
     5281 gtggaagtga aagggcagga tcgctatttt gatcgcgcgg ttcgtcgttt acagcagcag
     5341 ctgcgtaagc cagctgaaga actggggcga gagattactc atcagctatt cctgctgggc
     5401 tgcggtgcgc aaatgttgaa atatgcctct ccgccaatgg cgcaggcgtg gtgtcaggtg
     5461 atgttagata cgcgcggcgg cgtacggttg tcagagcaga tccagaatga tttattgctg
     5521 cgggcgacgg gaggagtgtg tttgtaagcg tatacgacgc tggtttcgat taactcaatg
     5581 aaatatgtga aaattgttag ccggacaagg cgctcgcgcc gcatccggca ttgttcattt
     5641 gctgtagatg acactcacgc atacaggatc gcctgtacgc gccagttatc aggctgctgg
     5701 tcttcgtaca tggtaataat gcggtaccat gacgcacctt gttcatcagc ctttaacgcc
     5761 acgatacgtt ccacgtcctg cggatcccct gaaatattgc taactgagat ctgacctatt
     5821 tcattcaggc ctgtcacgca gtcagcactg gcgaattctg cagactgtgc cgtggcgctc
     5881 gcaggataag tcactaattg cagcgaggat gaggcaagga attgtttcat cgtcagctcc
     5941 atttttcgtt tccccacatg ttgcagggca ttccatcgcg gtaataaacg cccaggcttc
     6001 cagccggtaa tttattgtgc acaggtaaac gcatagggac gcaaagcaat gtaaagctga
     6061 ctatgtaagt tgtctgattt atttacggta caaaatggct tgtgaatacc actgtcctgt
     6121 gacgatggtt tcgtccacca tgacgacaac gtaataatca gcttttgcag cgacagcttt
     6181 cgctctgatt tctgctaatg cgtcatccgg agaaccccga accatcgtgc ttacgctacc
     6241 tattcgctgt aacccttgcg tctggtcgcg gcgaatctct tgcggatggt cggttactgg
     6301 cggtgctggc tgtggcgtac cttgtagtgc gctacaggca cttaacatca acaccaataa
     6361 taaactggca aaccggtaaa taacgctatt acgtttcctg ctaaccatag tgtagtgcct
     6421 tattaattta actttggggg aatgttcccg aattgttacc tggtacgcga ttttcgaatg
     6481 aaaacgtgat gaaagcgtaa cccacatctc aacattatga actaataccg ccagcagaaa
     6541 cttgtgctaa ggatttcttc atgtcatacc agacaaaaag gagagacaga tgattgaaat
     6601 agaatcacgc gagctggcag atattcccgt tcttcatgct tatcctgtcg ggcaaaaaga
     6661 taccccgtta ccgtgcgcga ttttttatca cggctttact tcgtccagtc tggtgtatag
     6721 ctattttgcc gttgcgctgg cgcaggctgg tttgcgggtg atcatgccgg atgcgcccga
     6781 tcacggtagt cgttttagtg gcgacgcagc gcggcggtta aatcaattct ggcaaatctt
     6841 gctacaaagt atgcaggaat tcactacttt acgtgcggca atagccgaag aaaactggct
     6901 gcgtgatgac cgtctggcag tcggtggcgc gtcgatgggc gcgatgacgg cactgggaat
     6961 taccgctcgc cactccacgg tgaaatgtac cgccagcatg atgggatcgg gctattttac
     7021 atcgctcgcc cgttcactgt ttccaccgct gatacctgaa acggcagcac agcagaatga
     7081 attcaataac attgtcgcgc cgctggcaga gtgggaagcg acaaaccacc tggaacaact
     7141 tgctgacaga cctctactgc tgtggcatgg ccttgacgat gacgtcgtgc ctgccgacga
     7201 atcactacgt ttgcagcagg ccttaagcga gacgggacgg gataaactgc taacctgttc
     7261 atggcagcca ggcgtgcgtc accgcattac gcctgaggcg ttagatgctg ccgtgacatt
     7321 tttccgccag catctttaaa cacgcagaat gctgacccct tgcgcttcca gttgttgcag
     7381 gatttccggg ttagcatttt tgccggtgat cagcatatcg atttgatcgg cacggctaaa
     7441 aagcattccc gcgcgttcgc cgatcttact gctatcaacc agtaccacca gtttccctac
     7501 cacgcttagc atcttctgct ctgccattgc cgtcagcata tcggttttat acaacccttc
     7561 tgctgtcagc cctttgccgc tggtaaacat ccagtgcccg gcatagagac tgttttcgct
     7621 gccttgcggg cttaaagtga tggactgact tttgttgtac tgtccgccca taatgatcac
     7681 gctgtcatgc tcctgatcga tcaggtaatt tgccagcggt agataattag tgatgatttg
     7741 cactggcttg ccacacattt cccgcccaag cagaaacgcg gtggagccgc agttgatgac
     7801 tacgctttcg cccggattaa ccagctgcga ggccgcttta gcaatacgta ctttttcatc
     7861 gtgattctgc gcctgatgca gattcatcgg cgtccagcgt gggcgctgct gggtaatagc
     7921 ttctgcgcca ttgcgcactt ttttcagttt gccgctttcg tcaagtttat tgatatcgcg
     7981 tcgcgcagtg gcaggcgaaa ttcccagacg ctcaacgact ttctcaacgg tcacaaagcc
     8041 caattgtgcg agcatttcca ggaggatttg atgtctttgt gcttcagtca tgagctattc
     8101 cgataaaatt tgattttttt agatgatatt tgaaatagcc aggaaatact acgccggata
     8161 gcgcgaacta ctccacgcta tccggtacaa gcgacagatt acaggaacga cttgaacggc
     8221 agatccggtt caatagtgaa gcaatcatcg aaaccacgcg gatagtggta ctcgaagttg
     8281 tctttatcca gcggccaggt aaatttgccg ccgacctgcc agataaacgg cttgaagcca
     8341 tacttcaggc gatctttttt catctcccac agcacgcgga tctcttgcgg atcggcctgg
     8401 aagtttgacc agatatcgtg gtggaacggg atcactactt tagcattcag cgcttcgccc
     8461 atacgcaaga tatcagcact ggtcattttg tcggtaatac cgcgtgggtt ttcgccgtat
     8521 gaacctaaag ccacgtcgat ctgatgctcg ttgccatgtt ttgcatagta gttagagtag
     8581 tgggaatcgc cgctgtgata cagagaaccg cccggtgttt tgaacagata gtttactgcg
     8641 cgatcgtcca tgccgtctgg cagcacgcca gccgcttttt gatccgctgg tagggtgatc
     8701 agtgcagtac ggtcgaacgc gtcaagggca tgaatttcaa tgtctttcac ttttacgaca
     8761 tcgcccggtt tgaccacgat gcaacgctct ttcggtacgc cccagccaat ccataaatcc
     8821 acgcaggttt tcggtccgat aaacggtacg tcatcagcac agttctgcat cacggcagca
     8881 gcgacgttga cgtcgatatg atcgttgtga tcgtgagttg ccagtaccgc gtcgatctgg
     8941 cgaatcgcaa acggatcaag aacaaacggg gtggtgcgca ggtttggctg cagttttttc
     9001 acgccagcca tgcgctgcat ctggtgaccc tgtttcatca gcgggttacc gtgactttgt
     9061 ttgccagtac cgcaccagaa atcaacgcaa acgttggtgc caccttccga tttcagccag
     9121 atcccggtgc agcctagcca ccacatcgcg aatgtgccag gggcgacctg ctcttgttca
     9181 atttcttcat tcagccagct accccactcc gggaaagtgc tcaggatcca ggattctcgg
     9241 gtgatacttt tcactttact catcgccatt taccttcatg atagttcaat tcgaatcaac
     9301 atgtgattgg ttttgattaa tcctgacact attttttcag gaaggcaatg accatttttt
     9361 gactttttgc cagggaagtt gttgttgatt ttgagtgtgg aaagatttaa tggaatgtgt
     9421 aattcaatta actaaatgaa tttaaatgaa taattatttc gttgtgtgaa tcccactcta
     9481 tccatgtgga attatttgcg ggtcgcgtca catttaatca taaataatct tgttgtgatt
     9541 acttttgaaa attagagtga gtgcacaaca ttccgggtgt gtggaatacc cggttacctc
     9601 ttcttcagga gatcgttatg gagatcctct acaacatctt taccgtgttt tttaaccagg
     9661 tcatgaccaa tgccccgttg ttgctgggta ttgtgacctg tctgggctac atcctactgc
     9721 gcaaaagtgt cagcgttatt attaaaggca cgattaaaac cataattggt ttcatgttgt
     9781 tgcaggcagg atccggcatc ctcaccagca ccttcaaacc ggtggtggcg aaaatgtccg
     9841 aagtctacgg cattaacggc gcaatttccg atacttacgc gtcgatgatg gcaaccatcg
     9901 accgcatggg cgatgcctat agctgggtag gttacgcggt gttgttagcg ctggcactga
     9961 acatctgtta cgtgctgctg cgtcgcatta ccggtattcg taccatcatg ctgactggtc
    10021 acatcatgtt ccagcaggcg gggctgattg ccgtcacgct gttcatcttt ggctactcca
    10081 tgtggacgac cattatctgc accgcgattc tggtttcgct ctactggggc attacctcca
    10141 acatgatgta caagccgact caggaagtga cggacggctg cggtttctcc atcggtcacc
    10201 agcaacagtt tgcatcatgg attgcctata aatttgcgcc gttcctcggc aaaaaagagg
    10261 aaagcgttga agacctcaaa ctgccaggct ggctgaatat tttccacgac aacatcgtct
    10321 ccacggcgat tgtcatgacc atcttctttg gtgccattct gctctccttc ggtatcgaca
    10381 ccgtgcaggc gatggcaggc aaagtgaact ggacggtgta catcctgcaa actggtttct
    10441 ccttcgcggt ggcgatcttc atcatcactc agggtgtgcg catgtttgtg gcggaactct
    10501 ctgaagcgtt taacggtatc tcccagcgtc tgatccctgg tgcggttctg gcgattgact
    10561 gtgcggctat ttatagcttc gcgccgaacg ccgtggtctg gggctttatg tggggcacca
    10621 tcggtcagct gattgcggtt ggcatcctgg tcgcctgcgg ttcctcgatc ctgattatcc
    10681 caggctttat cccgatgttc ttctccaacg ccactattgg cgtgttcgct aaccacttcg
    10741 gcggctggcg tgcggcgctg aagatttgtc tggtgatggg gatgatcgaa atctttggct
    10801 gcgtctgggc ggtgaaactt accggtatga gtgcctggat gggtatggcg gactggtcga
    10861 ttctggcacc gccgatgatg cagggcttct tctccatcgg tatcgccttt atggctgtca
    10921 tcattgtaat tgcactggct tatatgttct tcgctggccg cgcactgcgc gcagaagaag
    10981 atgcagaaaa acaactggca gaacagtctg cttaataagg agttttgatt atgaccgtac
    11041 gtattctggc tgtgtgtggc aacggacaag gcagttccat gatcatgaaa atgaaagtgg
    11101 accagttttt aacccagtcg aacattgacc acacggtaaa cagctgcgcg gttggcgaat
    11161 acaaaagcga gttgagcggc gcggatatca tcatcgcttc tacccatatt gcgggcgaaa
    11221 tcactgtgac cggcaacaaa tatgtggttg gcgtgcgcaa tatgctctct cctgccgact
    11281 ttggcccgaa attgctggaa gtgatcaaag agcattttcc gcaggacgtg aagtaaggac
    11341 gcgccatgaa attacgtgat tcgctggcgg agaataaatc catccgcctg caggctgaag
    11401 ctgagacatg gcaggacgca gtaaaaatcg gcgttgacct gctggtggcg gcggatgttg
    11461 tcgagccgcg ttactaccag gcgattctgg atgcggttga acagcacggt ccgtactttg
    11521 tgctcgcacc cggcctggca atgccgcacg gtcgcccgga agagggcgtt aagaaaaccg
    11581 gttttgcgct ggtgacgctg aaaaagccgc tggagttcaa ccacgaagat aacgatccgg
    11641 tggatatcct catcaccatg gcggcggtcg atgccaacac tcaccaggaa gtgggcatca
    11701 tgcagatcgt caatctgttt gaagatgaag agaatttcga ccgtttacgt gcctgccgta
    11761 ccgagcagga agtactggat ctcattgacc gcaccaacgc ggcagcttaa gaaggaattg
    11821 aaaatgtcat taccgatgtt gcaggtcgcg ctggacaacc agactatgga tagcgcctat
    11881 gaaaccactc gcctgattgc cgaagaagtc gacattatcg aagtgggtac cattctgtgc
    11941 gtgggcgaag gcgtgcgtgc ggttcgcgat ctgaaagcgc tttacccgca caaaatcgta
    12001 ctggcagacg ccaaaattgc cgatgcgggc aaaatccttt ctcgtatgtg cttcgaagct
    12061 aacgctgact gggtgaccgt gatttgctgt gcggatatca acacagctaa aggtgcgctg
    12121 gacgtggcaa aagagtttaa cggcgacgtg cagatcgaac tgaccggtta ctggacctgg
    12181 gaacaggcgc aacagtggcg cgacgcaggc attgggcagg tggtttatca ccgcagccgt
    12241 gacgcacagg ccgcaggtgt ggcgtggggc gaagcggaca ttaccgcgat caaacgtctt
    12301 tccgatatgg gcttcaaagt caccgtcacc ggtggtctgg cgctggaaga tctgccgttg
    12361 tttaaaggca ttccgattca tgtgtttatc gctggtcgca gcattcgtga tgccgcttct
    12421 ccggtggagg ccgcacgtca gttcaaacgt tccatcgctg aactgtgggg ctaaggagcg
    12481 gatatgttgt ccaaacaaat cccgcttggc atctatgaaa aagcgctccc cgccggggag
    12541 tgctggctgg aacggctgca actggcaaaa acgttaggct tcgattttgt cgagatttcg
    12601 gtagatgaaa cggacgatcg cctgtcgcgc ctcgactgga gccgcgagca gcgtctggcg
    12661 ctggtgaacg cgattgttga aaccggcgtg cgcgtgccgt ccatgtgcct ttctgctcat
    12721 cgtcgtttcc cgctgggcag cgaagatgac gcagtgcggg cgcaggggct ggagattatg
    12781 cgcaaagcta tccagttcgc ccaggatgtc ggtattcgcg tgatccaact ggcgggctac
    12841 gacgtttact atcaggaagc caataacgaa acgcgtcgtc gtttccgtga cggcctgaaa
    12901 gagagcgttg agatggcaag ccgcgcgcag gtgacgctgg cgatggagat catggattac
    12961 ccgttgatga actccatcag caaggcgctg ggttacgcgc actatcttaa caacccgtgg
    13021 ttccagctct acccggatat cggcaacttg tcggcgtggg acaacgacgt gcagatggag
    13081 ttgcaggccg ggatcgggca tatcgtcgcg gtgcatgtga aagacaccaa accaggcgtc
    13141 ttcaaaaacg tcccgtttgg cgaaggtgta gtggatttcg aacgttgctt cgaaacgctc
    13201 aaacagagtg gttattgcgg gccgtacctg attgagatgt ggagcgaaac ggcggaagac
    13261 ccggcggcag aagtggcgaa agcgcgtgat tgggtgaaag cgcgcatggc caaagccggc
    13321 atggtggagg cggcataatg caaaagctaa aacagcaggt atttgaagcc aatatggatc
    13381 tgccgcgcta cgggctggtg acctttacct ggggcaacgt cagcgccatt gaccgcgaac
    13441 gcgggctggt ggtgatcaag cccagcggcg ttgcctacga aaccatgaaa gcagacgata
    13501 tggtggtggt tgatatgagc ggcaaggtgg tggaagggga gtatcgtccg tcttccgaca
    13561 ccgcgacgca tcttgaactc taccgccgtt atccgtcgct tggcggcatt gtccataccc
    13621 attccacgca tgccaccgca tgggcgcagg cggggctggc tatcccggca ttaggcacca
    13681 cgcacgctga ctacttcttt ggcgatatcc cctgtacgcg cgggttaagc gaagaagaag
    13741 tgcaggggga atacgagcta aacactggca aagtgattat cgaaacgctg ggtgacgccg
    13801 agccgctgca tacgccggga attgtggtgt atcagcacgg gccattcgcc tgggggaaag
    13861 atgctcacga tgcggtgcat aacgcggtgg tgatggaaga agtggcgaaa atggcgtgga
    13921 ttgcccgcgg cattaacccg caactgaatc acatcgacag ctatctcatg aataaacact
    13981 tcatgcgtaa gcacggtccc aacgcctatt acgggcagaa gtaaaacacg cgctgcggag
    14041 atttccttct tcgggagata actggtctaa gtccgcagcc gtttttcaaa aaaagccccc
    14101 tgaaaagggg gcaaagcaaa ctatggcaat gtttcgttgg ttatacctgg tgctagcgat
    14161 aaatatccgc gctggcgtgc atattgccgt tactccccgg ttcccgcatc agaatcacgt
    14221 ggtagtacgt tgcgccctgc gcatctgtct cttcatttaa tgcctgacgc gcttcgcttt
    14281 cagtggcgaa attatgattg atataaatca cgcctaagct ttgcacatcg tccatatttc
    14341 tggcctggtg gttattaatt tcaatggctg cccatgtatt tgcacttagc aaaagcacag
    14401 ccagaagggc taaaacacgg ctgaacatag atacctcctc gacggctgac tttgtgtgct
    14461 ctcctcttgt ggtgatcttc tgatttaatt ttaatcaatg ataaagaagt ggatggtgac
    14521 catttctgat gcagttgttc aaaaaaacac catgatgaag tgtgatgaac ttcaaatcag
    14581 cgtgttagta cgatttggtg aaggatgaga tttatttgtc ggctggcctt gagtttagcg
    14641 gggtatgtca gtactataac gcgtcatttt tcagccgacc tttaacacgt tccttgcctc
    14701 cccgggattc ggctgaccca gacaggaggc tgaataatcc gtaaggagca attcgatgcg
    14761 tcattacgaa atcgttttta tggtccatcc tgaccagagc gaacaggttc cgggcatgat
    14821 cgagcgctac actgctgcca tcactggtgc agaaggcaag atccaccgtc tggaagactg
    14881 gggccgccgt cagctggctt acccgatcaa caaactgcac aaagcacact acgttctgat
    14941 gaacgttgaa gctccgcagg aagtgatcga tgagctggaa actaccttcc gcttcaacga
    15001 tgccgttatc cgcagcatgg ttatgcgtac caagcacgct gttaccgaag catctccgat
    15061 ggttaaagcg aaagacgagc gccgtgagcg tcgcgatgat ttcgcaaacg aaaccgctga
    15121 tgatgctgaa gctggggatt ctgaagagta atttctgatg accaaccgtc tggtgttgtc
    15181 cggcaccgtg tgcaggactc cccttcgaaa ggtcagtcca tcaggaattc ctcactgcca
    15241 gttcgtgctt gagcatcgtt ctgtgcagga ggaagccggc tttcaccggc aggcatggtg
    15301 ccaaatgccc gttattgtta gcggacacga aaaccaggcc attactcaca gtataacggt
    15361 cggcagtcgc ataaccgttc aggggttcat ttcatgccat aaggcaaaga acggactgag
    15421 caaaatggtt ttgcatgccg agcagattga attgatagat tctggagact agccatatgg
    15481 cacgttattt ccgtcgtcgc aagttctgcc gtttcaccgc ggaaggcgtt caagagatcg
    15541 actataaaga tatcgctacg ctgaaaaact acatcaccga aagcggtaag attgtcccaa
    15601 gccgtatcac cggtacccgt gcaaaatacc agcgtcagct ggctcgcgct atcaaacgcg
    15661 ctcgctacct gtccctgctg ccgtacactg atcgccatca gtaatcggtc acggtccatt
    15721 aatacgactt tgagaggata aggtaatgca agttattctg cttgataaag tagcaaacct
    15781 gggtagcctg ggtgatcagg taaacgttaa agcgggctat gctcgtaact tcctggtacc
    15841 gcagggtaaa gctgttccag ctaccaagaa aaacattgaa ttcttcgaag cacgtcgcgc
    15901 tgaactggaa gctaaactgg ctgaagttct ggcagcagct aatgctcgcg ctgagaaaat
    15961 caatgcactg gaaactgtta ccatcgcgtc taaagctggc gacgaaggta aactgttcgg
    16021 ttccatcggt actcgcgaca tcgctgacgc tgtaactgca gctggcgttg aagtggctaa
    16081 gagcgaagtt cgtctgccga acggcgttct gcgtaccact ggcgaacacg aagtgagctt
    16141 ccaggttcac agcgaagtat tcgcgaaagt gatcgtaaac gtagtagctg aataattcgt
    16201 tattcaacga gacgtaaaaa cgccgaccag tggtcggcgt tttgctttta agtgataaaa
    16261 accgcagaaa cagacgttcc ctccataaat ctcttgctat tccttttata aaaagttcgc
    16321 agtacccata cctggccccc agcctcgccg ctggtagttc gtgtcatgtt ttttacgcgt
    16381 aaaacacgac aaaagcatca atacagcgat cgtctcac
//
LOCUS       DAZFLD010000110        16374 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.111, whole genome shotgun sequence.
ACCESSION   DAZFLD010000110 DAZFLD010000000
VERSION     DAZFLD010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16374
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.111"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(29..1483)
                     /gene="xylB"
                     /locus_tag="ACHKBL_002951"
     CDS             complement(29..1483)
                     /gene="xylB"
                     /locus_tag="ACHKBL_002951"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - D-xylulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HFO5819797.1"
                     /translation="MYIGIDLGTSGVKVILLNEQGEVVASQTEKLTVSRPHPLWSEQD
                     PEQWWQATDRAMKALGDQHSLQDVKALGIAGQMHGATLLDAQQRVLRPAILWNDGRCA
                     QECTLLEARVPQSRVITGNLMMPGFTAPKLLWVQRHEPEIFRQIDKVLLPKDYLRLRM
                     TGEFASDMSDAAGTMWLDVAKRDWSDDMLQACHLSRDQMPALYEGSEITGALLPEVAK
                     AWSMAAVPVVAGGGDNAAGAVGVGMVDANQAMLSLGTSGVYFAVSEGFLSKPESAVHS
                     FCHALPQRWHLMSVMLSAASCLDWAAKLTGLSNVPALIAAAQQADESAEPVWFLPYLS
                     GERTPHNNPQAKGVFFGLTHQHGPNELARAVLEGVGYALADGMDVVHACGIKPQSVTL
                     IGGGARSEYWRQMLADISGQQLDYRTGGDVGPALGAARLAQIAANPEKSLIELLPQLP
                     LEQSHLPDAQRYAAYQPRRETFRRLYQQLLPLMA"
     gene            complement(1555..2877)
                     /gene="xylA"
                     /locus_tag="ACHKBL_002952"
     CDS             complement(1555..2877)
                     /gene="xylA"
                     /locus_tag="ACHKBL_002952"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009045 - xylose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HFO5819798.1"
                     /translation="MQAYFDQLDRVRYEGSKSSNPLAFRHYNPDELVLGKRMEEHLRF
                     AACYWHTFCWNGADMFGVGAFNRPWQQPGEALALAKRKADVAFEFFHKLHVPFYCFHD
                     VDVSPEGASLKEYINNFAQMVDVLAGKQEESGVKLLWGTANCFTNPRYGAGAATNPDP
                     EVFSWAATQVVTAMEATHKLGGENYVLWGGREGYETLLNTDLRQEREQLGRFMQMVVE
                     HKHKIGFQGTLLIEPKPQEPTKHQYDYDAATVYGFLKQFGLEKEIKLNIEANHATLAG
                     HSFHHEIATAIALGLFGSVDANRGDAQLGWDTDQFPNSVEENALVMYEILKAGGFTTG
                     GLNFDAKVRRQSTDKYDLFYGHIGAMDTMALALKIAARMIEDGELDKRIAQRYSGWNS
                     ELGQQILKGQMSLADLAKYAQEHNLSPVHQSGRQEQLENLVNHYLFDK"
     gene            3243..4235
                     /gene="xylF"
                     /locus_tag="ACHKBL_002953"
     CDS             3243..4235
                     /gene="xylF"
                     /locus_tag="ACHKBL_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015753 - D-xylose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO5819799.1"
                     /translation="MKIKNILLTLCTSLLLTNVAAHAKEVKIGMAIDDLRLERWQKDR
                     DIFVKKAESLGAKVFVQSANGNEETQMSQIENMINRGVDVLVIIPYNGQVLSNVVKEA
                     KQEGIKVLAYDRMINDADIDFYISFDNEKVGELQAKALVDIVPQGNYFLMGGSPVDNN
                     AKLFRAGQMKVLKPYVDSGKIKVVGDQWVDGWLPENALKIMENALTANNNKIDAVVAS
                     NDATAGGAIQALSAQGLSGKVAISGQDADLAGIKRIAAGTQTMTVYKPITLLANTAAE
                     IAVELGNGQEPKADTTLNNGLKDVPSRLLTPIDVNKNNIKDTVVKDGFHKESEL"
     gene            4313..5854
                     /gene="xylG"
                     /locus_tag="ACHKBL_002954"
     CDS             4313..5854
                     /gene="xylG"
                     /locus_tag="ACHKBL_002954"
                     /EC_number="7.5.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015614 - ABC-type D-xylose transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015753 - D-xylose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter ATP-binding protein"
                     /protein_id="HFO5819800.1"
                     /translation="MPYLLEMKNITKTFGSVKAIDNVSLRLNAGEIVSLCGENGSGKS
                     TLMKVLCGIYPHGSYEGEIIFAGEEIQASHIRDTERKGIAIIHQELALVKELTVLENI
                     FLGNEITHNGIMDYDLMTLRCQKLLAQVSLSISPDTRVGDLGLGQQQLVEIAKALNKQ
                     VRLLILDEPTASLTEQETSVLLDIIRDLQQHGIACIYISHKLNEVKAISDTICVIRDG
                     QHIGTRDAAGMSEDDIITMMVGRELTALYPNEPHTTGDEILRIEHLTAWHPVNRHIKR
                     VNDVSFSLKRGEILGIAGLVGAGRTETIQCLFGVWPGQWEGKIYIDGKQVDIRNCQQA
                     IAQGIAMVPEDRKRDGIVPVMAVGKNITLAALKKFTGGISQLDDAAEQKCILESIQQL
                     KVKTSSPDLAIGRLSGGNQQKAILARCLLLNPRILILDEPTRGIDIGAKYEIYKLINQ
                     LVQQGIAVIVISSELPEVLGLSDRVLVMHEGKLKANLINHNLTQEQVMEAALRSEHHV
                     EKQSV"
     gene            5832..7013
                     /gene="xylH"
                     /locus_tag="ACHKBL_002955"
     CDS             5832..7013
                     /gene="xylH"
                     /locus_tag="ACHKBL_002955"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose ABC transporter permease XylH"
                     /protein_id="HFO5819801.1"
                     /translation="MSKSNPSEVKLAVPTSGGFSGLKSLNLQVFVMIAAIIAIMLFFT
                     WTTDGAYLSARNVSNLLRQTAITGILAVGMVFVIISAEIDLSVGSMMGLLGGVAAICD
                     VWLGWPLPLTIIVTLVLGLLLGAWNGWWVAYRKVPSFIVTLAGMLAFRGILIGITNGT
                     TVSPTSAAMSQIGQSYLPASTGFIIGALGLMAFVGWQWRGRMRRQALGLQSPASTAVV
                     GRQALTAIIVLGAIWLLNDYRGVPTPVLLLTLLLLGGMFMATRTAFGRRIYAIGGNLE
                     AARLSGINVERTKLAVFAINGLMVAIAGLILSSRLGAGSPSAGNIAELDAIAACVIGG
                     TSLAGGVGSVAGAVMGAFIMASLDNGMSMMDVPTFWQYIVKGAILLLAVWMDSATKRR
                     S"
     gene            7091..8269
                     /gene="xylR"
                     /locus_tag="ACHKBL_002956"
     CDS             7091..8269
                     /gene="xylR"
                     /locus_tag="ACHKBL_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312479.1"
                     /note="D-xylose enhances binding of XylR to the xyl
                     promoter and activates transcription; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose utilization transcriptional activator
                     XylR"
                     /protein_id="HFO5819802.1"
                     /translation="MFTKRHRITLLFNANKAYDRQVVEGVGEYLQASQSEWDIFIEED
                     FRARIDKIKDWLGDGVIADFDDKQIEQALADVDVPIVGVGGSYHLAESYPPVHYIATD
                     NYALVESAFLHLKEKGVNRFAFYGLPESSGKRWATEREYAFRQLVAEEKYRGVVYQGL
                     ETAPENWQHAQNRLADWLQTLPPQTGIIAVTDARARHILQVCEHLHIPVPEKLCVIGI
                     DNEELTRYLSRVALSSVAQGARQMGYQAAKLLHRLLDKEEMPLQRILVPPVRVIERRS
                     TDYRSLTDPAVIQAMHYIRNHACKGIKVDQVLDAVGISRSNLEKRFKEEVGETIHAMI
                     HAEKLEKARSLLISTTLSINEISQMCGYPSLQYFYSVFKKAYDTTPKEYRDVNSEVML
                     "
     gene            complement(8377..9201)
                     /locus_tag="ACHKBL_002957"
     CDS             complement(8377..9201)
                     /locus_tag="ACHKBL_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein bax"
                     /protein_id="HFO5819803.1"
                     /translation="MILTPIRRYGAMILMLLTLVFSSEVLAKTHTTTASQKSHLTKAS
                     NKQVSSKQEYSRNSAKSSSLPDLRKYPSGTPRKKAFLRTVMPYITSQNAAITAERNWL
                     ISKQYQGQWSPAERARLKDIAKRYKVKWSGNTRKIPWNTLLERVDIIPTSMVATMAAA
                     ESGWGTSKLARNNNNLFGMKCMKGRCTNAPGKVKGYSQFSSVKESVSAYVTNLNTHPA
                     YSSFRKSRAQLRKADQEVTATAMIHKLKGYSTKGKSYNNYLFAMYQDNQRLIAAHM"
     gene            9521..11548
                     /gene="malS"
                     /locus_tag="ACHKBL_002958"
     CDS             9521..11548
                     /gene="malS"
                     /locus_tag="ACHKBL_002958"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004556 - alpha-amylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HFO5819804.1"
                     /translation="MKLAACFLTLLPGFAVAASWTSPGFPTFSEQGTGTFVSHAQLPK
                     GTRPLTLNFDQQCWQPADAIKLNQMLSLQPCSNTPPQWRLFRDGEYTLQLDTRSGTPT
                     LMISIQNAVEPVASLVRECPKWDGLPLTLDVSATFPEGAAVRDYYSQQIAIVKNGQIT
                     LQPAATSNGLLLLERAETDAPAPFDWHNATVYFVLTDRFENGDPSNDQSYGRHKDGMA
                     EIGTFHGGDLRGLTNKLDYLQQLGVNALWISAPFEQIHGWVGGGTKGDFPHYAYHGYY
                     TQDWTNLDANMGSEADLRTLVDSAHQRGIRILFDVVMNHTGYATLADMQEYQFGALYL
                     SGDEVKKTLGERWSDWKPAAGQTWHSFNDYINFSDKTGWDKWWGKNWIRTDIGDYDNP
                     GFDDLTMSLAFLPDIKTESTTASGLPVFYKNKTDTHAKVIDGFTPRDYLTHWLSQWVR
                     DYGIDGFRVDTAKHVELPAWQQLKTEASAALVEWKKANPDKALDDKPFWMTGEAWGHG
                     VMQSDYYRHGFDAMINFDYQEQAAKAVDCLAQMDTTWQQMAEKLQDFNVLSYLSSHDT
                     RLFREGGDKAAELLLAPGAVQIFYGDESSRPFGPTGSDPLQGTRSDMNWQDVSGKSAA
                     SVAHWQKISQFRARHPAIGAGKQTTLSLKQGYGFVREHGDDKVLVIWAGQQ"
     gene            11726..12979
                     /gene="avtA"
                     /locus_tag="ACHKBL_002959"
     CDS             11726..12979
                     /gene="avtA"
                     /locus_tag="ACHKBL_002959"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HFO5819805.1"
                     /translation="MTFSLFGDKFTRHSGITLLMEDLNDGLRTPGAIMLGGGNPAQIP
                     EMQDYFQTLLTDMLESGKATDALCNYDGPQGKTELLTLLAGMLREKLGWDIEPQNIAL
                     TNGSQSAFFYLFNLFAGRRADGRIKKVLFPLAPEYIGYADAGLEEDLFVSARPNIELL
                     PEGQFKYHVDFEHLHIGEETGMICVSRPTNPTGNVITDEELLKLDALANQHGIPLVID
                     NAYGVPFPGIIFSEARPLWNPNIVLCMSLSKLGLPGSRCGIIIANEKIITAITNMNGI
                     ISLAPGGIGPAMMSEMIKRNDLLRLSETVIKPFYYQRVQETIAIIRRYLPEDRCLIHK
                     PEGAIFLWLWFKDLPITTEQLYQRLKARGVLMVPGHNFFPGLDKPWPHTHQCMRMNYV
                     PEPEKIEAGVKILAEEIERAWAESH"
     gene            complement(13131..13604)
                     /locus_tag="ACHKBL_002960"
     CDS             complement(13131..13604)
                     /locus_tag="ACHKBL_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFO5819806.1"
                     /translation="MNRFIIADATKCIGCRTCEVACAVSHQENQDCAALSPDEFISRI
                     RVIKDHTWTTAVACHQCEDAPCANVCPVDAISREHGHIFVEQSRCIGCKSCMLACPFG
                     AMEVVSSRKKARAIKCDLCWHRATGPACVEACPTKALQCMDVEKVQRHRLRQQPV"
     gene            complement(13706..14554)
                     /gene="yiaJ"
                     /locus_tag="ACHKBL_002961"
     CDS             complement(13706..14554)
                     /gene="yiaJ"
                     /locus_tag="ACHKBL_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator YiaJ"
                     /protein_id="HFO5819807.1"
                     /translation="MGKEVMGKKENEMAQEKERPAGSQSLFRGLMLIEILSNYPNGCP
                     LAHLSELAGLNKSTVHRLLQGLQSCGYVTTAPAAGSYRLTTKFIAVGQKALSSLNIIH
                     IAAPHLEALNIATGETINFSSREDDHAILIYKLEPTTGMLRTRAYIGQHMPLYCSAMG
                     KIYMAFGHPDYVKSYWESHQHEIQPLTRNTITELPAMFDELAHIRESGAAMDREENEL
                     GVSCIAVPVFDIHGRVPYAVSISLSTSRLKQVGEKNLLKPLRETAQAISNELGFTVRD
                     DQGAIT"
     gene            14755..15753
                     /gene="yiaK"
                     /locus_tag="ACHKBL_002962"
     CDS             14755..15753
                     /gene="yiaK"
                     /locus_tag="ACHKBL_002962"
                     /EC_number="1.1.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047559 - 3-dehydro-L-gulonate
                     2-dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydro-L-gulonate 2-dehydrogenase"
                     /protein_id="HFO5819808.1"
                     /translation="MKVTFEQLKAAFNRVLISRDVDSETADACAEMFARTTESGVYSH
                     GVNRFPRFIQQLENGDIIPDAQPKRITSLGAIEQWDAQRSIGNLTAKKMMDRAIELAA
                     DHGIGLVALRNANHWMRGGSYGWQAAEKGYIGICWTNSIAVMPPWGAKECRIGTNPLI
                     VAIPSTPITMVDMSMSMFSYGMLEVNRLAGRQLPVDGGFDDEGNLTKEPGVIEKNRRI
                     LPMGYWKGSGMSIVLDMIATLLSDGASVAEVTQDNSDEYGISQIFIAIEVDKLIDGPT
                     RDAKLQRIMDYVTTAERADENQAIRLPGHEFTTLLAENRRNGITVDDSVWAKIQAL"
     gene            15765..16232
                     /locus_tag="ACHKBL_002963"
     CDS             15765..16232
                     /locus_tag="ACHKBL_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HFO5819809.1"
                     /translation="MIFGHIAQPDPCRLPAAIEKALDFLRTTDFNALEPGVVEIDGKN
                     IYAQIIDLTTREAAENRPEVHRRYIDIQFLAWGEEKIGIAIDTGNNKVSESLLEQRDI
                     IFYHDSEHESFIEMIPGSYAIFFPQDVHRPGCILQTTSEIRKIVVKVALTALN"
BASE COUNT         4184 a         4148 c         4016 g         4026 t
ORIGIN      
        1 ccccacccgg tcaggcagag gataacgttt acgccattaa tggcagaagt tgctgataga
       61 ggcgacggaa cgtttctcgt cgtggctgat aagcggcata acgctgcgca tctggtagat
      121 gcgactgttc taacggtaac tgcggcaaca actcaatgag cgatttctct ggattcgccg
      181 caatctgcgc cagccttgct gcgcccagtg ctggccccac atcccccccc gtacggtaat
      241 cgagctgctg accgctgata tccgccagca tttgacgcca gtactcacta cgcgccccgc
      301 caccaatcaa cgtaacactt tgcggtttaa tgccgcaggc atgcacgaca tccatgccat
      361 ctgccagcgc ataacccacg ccttccagca ctgctcgcgc cagttcattg gggccatgtt
      421 gatgagtcaa accaaagaaa acccccttcg cctggggatt attgtgtggc gtacgctcgc
      481 cggaaagata aggcagaaac caaactggct cggcactttc atcagcctgt tgagctgcag
      541 cgattaaagc tgggacattg ctcaggccgg ttaatttcgc ggcccaatcc agacacgacg
      601 ctgcactcag cattacagac attaaatgcc aacgctgcgg tagcgcatgg caaaagctgt
      661 gtacggcgct ttctggcttg cttaaaaacc cttcgctgac agcaaaatag acccccgacg
      721 tccccagcga taacattgcc tgattagcat caaccattcc tacaccaacc gcaccagctg
      781 cattatcacc accgcctgcg acaactggca ccgccgccat actccacgct ttcgcaactt
      841 caggtagcaa agcaccagta atttcgctgc cttcgtataa tgcgggcatc tggtcacgag
      901 ataagtggca agcctgcagc atgtcgtcac tccagtcacg ctttgcgaca tccagccaca
      961 tggtgccagc tgcgtcagac atatcgctgg caaactcccc cgtcatacgc agacgcaagt
     1021 aatctttcgg taataatact ttgtcgattt gacggaatat ctccggctca tgccgctgaa
     1081 cccatagcaa tttaggcgca gtaaatccgg gcatcatcag gttaccggta atcacccgtg
     1141 attgcggaac tcgcgcttcc agcaaagtgc actcttgcgc acagcgcccg tcgttccaca
     1201 aaatggcagg gcgtaatacc cgttgctggg catccagtaa ggttgcaccg tgcatctggc
     1261 cggcaatacc caatgcttta acgtcctgca gagaatgctg atcgcccaga gctttcattg
     1321 cgcggtcagt tgcctgccac cactgttccg ggtcttgttc cgaccagagt ggatgcgggc
     1381 gcgaaacggt cagcttttcc gtttgcgaag caaccacctc accctgctcg ttgagcaaaa
     1441 taacttttac gcccgaggtg ccaagatcta tcccgatata catatcgatc gttccttaaa
     1501 aaaatgcccg gtatcgctac cgataaccgg gccaacggac tgcacagtta gccgttattt
     1561 gtcgaacaga taatgattta ccagattttc cagttgctcc tggcgaccac tctgatgcac
     1621 cggagacaaa ttgtgttcct gagcatattt ggctaaatct gccagtgaca tttggccttt
     1681 caggatttgc tggcctaatt cgctattcca gccggaataa cgctgcgcga tgcgtttatc
     1741 cagctcgcca tcttcaatca tgcgtgctgc aattttcagc gccaacgcca tcgtatccat
     1801 cgcgccgata tgaccgtaaa acagatcata tttatcagta ctttgacgac gtactttggc
     1861 atcgaagttc agaccgccag tggtgaaacc gcctgctttg agaatttcat acatcaccag
     1921 cgcattctct tccacactgt tcgggaactg gtcggtgtcc cagcccagtt gtgcatcgcc
     1981 acggttggcg tcgacagaac cgaacaggcc aagcgcaatg gcggtggcta tttcatgatg
     2041 gaaagagtga cctgccagcg tcgcgtggtt agcttcaata ttcagtttaa tctctttttc
     2101 cagaccaaac tgtttcagga agccatagac cgtcgcagca tcgtaatcat attgatgttt
     2161 ggtcggttct tgcggtttcg gttcgataag caacgtgccc tggaaaccga ttttatgttt
     2221 atgctcaacc accatctgca taaagcggcc cagctgttca cgctcctggc gcaagtcggt
     2281 atttaacagc gtttcgtaac cttcacgacc accccacagg acatagtttt caccgcccaa
     2341 tttatgggtt gcttccatcg ctgtaacaac ttgcgttgcc gcccaactga agacttcagg
     2401 atctgggttc gtcgccgcac ccgcgccata gcgagggttt gtaaagcagt tagcagttcc
     2461 ccacagcagc ttcacgccgc tctcttcttg cttgcctgcc aggacatcaa ccatttgcgc
     2521 aaaattattg atgtactctt ttaacgacgc gccctcaggg gaaacatcca catcgtggaa
     2581 gcaataaaat ggcacatgta acttgtggaa aaactcaaat gcgacatctg ctttacgctt
     2641 cgccaacgcc agtgcctcac caggctgctg ccacggacga ttaaacgctc ccacaccaaa
     2701 catatccgcc ccgttccagc agaaggtgtg ccagtagcag gcggcaaaac gcaagtgctc
     2761 ttccatacgc ttgcccaaca ccagttcgtc gggattgtag tgacggaatg ctaacgggtt
     2821 tgaggatttt gagccttcat aacgaacgcg atcgagctgg tcaaaatagg cttgcatatt
     2881 gaactccata atcaggtaat gccgcgggtg atggatgatg tcgtaatatt gggcactccc
     2941 tttcagttgc tcaattatgt tatttcacac tgctattgag ataattcaca agtgtgcgct
     3001 cgctcgcaaa ataaaatggg atgatgaaac tgggtaattc ctcgaagaga aaaatgcaat
     3061 aagtacaatt gcgcaacaaa agtaagatct cggtcataaa tcaagaaata aaccaaaaat
     3121 cgtaatcgaa agataaaaat ctgtaattgt tttcccctgt ttagttgcta aaaattggtt
     3181 acgtttatcg cggtgattgt tacttattaa agctgtcctc taactacaga aggccctaca
     3241 ccatgaaaat aaagaacatt ctactcaccc tttgcacctc acttctgctt accaacgttg
     3301 cggcacacgc caaagaagtc aaaataggta tggcgattga tgatctccgt cttgaacgct
     3361 ggcaaaaaga tcgagatatt tttgtgaaaa aagcagaatc tctcggcgcg aaagtatttg
     3421 tacagtctgc aaatggcaat gaagaaacac aaatgtcgca gattgaaaac atgattaacc
     3481 ggggtgtcga tgttcttgtc attattcctt ataacggtca ggtattaagt aacgttgtaa
     3541 aagaagccaa acaagaaggt attaaagtat tagcttacga ccgtatgatt aacgatgcgg
     3601 atatcgattt ttatatttct ttcgataacg aaaaagtcgg tgaactgcag gcaaaagccc
     3661 tggtcgatat tgttccgcaa ggtaattact tcctgatggg cggctcgccg gtagataaca
     3721 acgctaagct gttccgcgcc ggacaaatga aagtgttaaa accttacgtt gattctggaa
     3781 aaattaaagt cgttggtgac caatgggttg atggctggtt accggaaaac gcattgaaaa
     3841 ttatggaaaa cgcgctaacc gccaataata acaaaattga tgctgtagtt gcctcaaacg
     3901 atgccaccgc aggtggggca attcaggcat taagcgcgca aggtttatca gggaaagtag
     3961 caatttccgg ccaggatgcg gatctcgcag gtattaaacg tattgctgcc ggtacgcaaa
     4021 ctatgacggt gtataaacct attacgttgt tggcaaatac tgccgcagaa attgccgttg
     4081 agttgggcaa tggtcaggaa ccaaaagcag ataccacact gaataatggc ctgaaagatg
     4141 ttccctcccg cctgctgaca ccgatcgatg tgaataaaaa caacatcaaa gacacggtag
     4201 ttaaagacgg attccacaaa gagagcgagc tgtaagcgtt acgccccggc gaggagcggg
     4261 ggcgtgattt ctctccatgc cgtgtgaatt aattggctta ggtggagtcg ttatgcctta
     4321 tctacttgaa atgaagaaca ttaccaaaac cttcggcagt gtgaaggcga ttgataacgt
     4381 cagcttgcgg ttgaatgctg gcgaaatcgt ctcactctgt ggggaaaatg ggtctggtaa
     4441 atcaacgctg atgaaagtgc tgtgtggtat ttatccccat ggttcctacg aaggcgaaat
     4501 tatttttgcg ggagaagaga ttcaggcgag tcacatccgc gataccgaac gcaaaggtat
     4561 cgccatcatt caccaggaat tggccctggt gaaagaattg accgtgctgg aaaatatctt
     4621 cctgggtaac gaaataaccc ataatggcat tatggattat gatctgatga cgctacgctg
     4681 tcagaagctg ctcgcacagg tcagtttatc catttcacct gatacccgcg ttggcgattt
     4741 aggacttggg caacaacaac tggttgaaat tgccaaggca cttaataaac aggtgcgttt
     4801 gttaattctc gatgagccga cagcctcatt aactgagcag gaaacgtcgg ttttactgga
     4861 tattattcgc gatctacaac agcacggtat cgcctgtatt tatatttcgc acaaactcaa
     4921 cgaagtcaaa gcgatttccg atacgatttg cgttattcgc gacggtcagc acattggcac
     4981 gcgtgatgct gctggaatga gtgaagacga tattatcacc atgatggtcg gacgagagtt
     5041 aaccgcgctt taccctaatg aaccacatac caccggagat gaaatattac gtattgaaca
     5101 tctgacggca tggcatccgg ttaatcgtca tattaaacga gttaatgatg tctcgttttc
     5161 cctgaaacgt ggcgaaatac tgggtatcgc cggactcgtt ggtgccggac gtaccgagac
     5221 tattcagtgc ctgtttggtg tgtggcccgg acaatgggaa gggaaaattt atattgatgg
     5281 caaacaggta gatattcgta actgtcagca agccatcgcc caggggattg cgatggtacc
     5341 cgaagacaga aagcgcgacg gcatcgttcc ggtaatggcg gttggtaaaa atattaccct
     5401 cgccgcactc aaaaaattta ccggtggcat tagccagctt gatgacgcgg cagagcaaaa
     5461 atgtattctg gaatcaatcc agcaactcaa agttaaaacg tcgtcccccg accttgctat
     5521 tggacgtttg agcggcggca atcagcaaaa agcgatcctc gctcgctgtc tgttacttaa
     5581 cccgcgcatt ctcattcttg atgaacccac caggggtatc gatattggcg cgaaatacga
     5641 gatctacaaa ttaattaacc aactcgtcca gcagggtatt gccgttattg tcatctcttc
     5701 cgaattacct gaagtgctcg gccttagcga tcgtgtactg gtgatgcatg aagggaaact
     5761 aaaagccaac ctgataaatc ataacctgac tcaggagcag gtgatggaag ccgcattgag
     5821 gagcgaacat catgtcgaaa agcaatccgt ctgaagtgaa attagccgta ccgacatccg
     5881 gtggcttttc cgggctgaaa tcactgaatt tgcaggtctt cgtgatgatt gcagctatca
     5941 tcgcaatcat gctgttcttt acctggacca ccgatggtgc ctacttaagc gcccgtaacg
     6001 tctccaacct gttacgccaa accgcgatta ccggcatcct cgcggtagga atggtgttcg
     6061 tcataatttc tgctgaaatc gacctttccg tcggctcaat gatggggctg ttaggtggcg
     6121 tcgcggcgat ttgtgacgtc tggctaggct ggcctttgcc acttaccatc attgtaacgc
     6181 tggttctggg attgcttctc ggtgcctgga acggatggtg ggtcgcgtac cgcaaagtcc
     6241 cttcatttat tgtcaccctc gcgggcatgt tggcatttcg cggcatactc attggcatca
     6301 ccaacggcac gaccgtatcc cccaccagcg ccgcgatgtc acaaattggg caaagctatc
     6361 tacctgccag cactggcttc atcattggcg cgcttggctt aatggctttt gttggctggc
     6421 aatggcgcgg aagaatgcgc cgtcaggctt tgggtttgca atctcccgcc tctaccgcag
     6481 tagttggtcg ccaggcttta accgctatca tcgtattagg cgcaatctgg ctgttgaatg
     6541 attaccgtgg cgttcccact cctgttctgc tgctgacgtt gctgttactc ggcggaatgt
     6601 ttatggcaac gcggacagca tttggacgac gcatttatgc catcggcggc aatctggaag
     6661 cggcacgtct ctccgggatt aacgttgaac gcaccaaact tgccgtgttc gcgattaacg
     6721 gattaatggt agccatcgcc ggattaatcc tcagttctcg acttggcgct ggttcacctt
     6781 ctgcggggaa tatcgccgag ctggacgcaa ttgcagcatg tgtgattggc ggcaccagtc
     6841 tggcaggcgg cgtcgggagc gtggctggag cggtaatggg ggcattcatc atggcttcac
     6901 tggataacgg catgagtatg atggatgtac cgaccttctg gcagtatatc gttaaaggtg
     6961 cgattctgtt gctggctgta tggatggact ccgcaaccaa acgccgttct tgattttgat
     7021 aaaaattttc tcaaagccgg taacgtatta ccggttttga gtttttgcat gattcagcag
     7081 gaaaagaacc atgtttacta aacgtcaccg catcacatta ctgttcaatg ccaataaagc
     7141 ctatgaccgg caggtagtag aaggcgtagg ggaatattta caggcgtcac aatcggaatg
     7201 ggatattttc attgaagaag atttccgcgc ccgcattgat aaaatcaagg actggttagg
     7261 agatggcgtc attgccgact tcgacgacaa acaaatcgag caagcgctgg ctgatgtcga
     7321 cgtccccatt gttggggttg gtggctcgta tcaccttgcc gaaagttacc cacccgttca
     7381 ttacattgcc accgataact atgcgctggt tgaaagcgca tttttgcatt taaaagagaa
     7441 aggcgttaac cgctttgctt tttatggtct tccggaatca agcggtaaac gttgggccac
     7501 tgaacgcgaa tatgcatttc gtcagcttgt cgccgaagaa aagtatcgcg gagtggttta
     7561 tcaggggtta gaaaccgcgc cagagaactg gcaacacgcg caaaatcggc tggcagactg
     7621 gctacaaacg ctgccaccgc aaaccgggat tattgccgtt actgacgccc gggcgcggca
     7681 tattctgcaa gtatgtgaac atctacatat tcccgtaccg gaaaaattat gcgtgattgg
     7741 catcgataac gaagaactga cccgctatct gtcgcgtgtc gccctttctt cggtcgctca
     7801 gggcgcgcgg caaatgggct atcaggcggc aaaactgttg catcgattat tagataaaga
     7861 agaaatgccg ctacagcgga ttttggtccc gccagttcgc gtcattgaac ggcgctcaac
     7921 agattaccgc tcgctgaccg atcccgccgt tattcaggcc atgcattaca ttcgtaatca
     7981 cgcctgtaaa gggattaaag tggatcaggt actggatgcg gtcgggatct cgcgctccaa
     8041 tcttgagaag cgttttaaag aagaggtggg tgaaaccatc catgccatga ttcatgccga
     8101 gaagctggag aaagcgcgca gtctgctgat ttcaaccacc ttgtcgatca atgagatatc
     8161 gcaaatgtgc ggttatccat cgctgcaata tttctactct gtttttaaaa aagcatatga
     8221 cacgacgcca aaagagtatc gcgatgtaaa tagcgaggtc atgttgtagc gatgatgaga
     8281 attgtcggcg tcgcatcagg taactcagca caaccacctg atgcaagcaa aagccttatc
     8341 cgactgacaa gtcggataag gctcgaggaa atgcaattac atatgcgccg cgattaaccg
     8401 ttggttatcc tggtacattg cgaacaggta gttgttataa ctcttccctt tagtcgagta
     8461 gcccttcagc ttgtgaatca tcgctgtggc agtcacttcc tgatccgctt tacgcagctg
     8521 tgcacgcgat ttacggaacg aagagtaagc cggatgagta ttcaggttag tgacataggc
     8581 gctcaccgat tctttgacag aactaaactg tgagtaccct ttcactttac ccggcgcatt
     8641 ggtacaacgt cctttcatgc atttcatgcc gaacagattg ttgttgttgc gcgccagctt
     8701 tgacgttccc caaccgcttt ctgctgcagc catcgtcgcc accatactgg tagggataat
     8761 gtctacgcgt tcaagcaagg tattccacgg gatttttcgc gtattaccag accacttcac
     8821 cttgtagcgt ttggcgatgt ctttcagacg cgcacgctca gcaggtgacc attggccctg
     8881 atactgtttt gaaatcagcc agttacgttc cgcagtaatg gccgcatttt ggctggtaat
     8941 gtaaggcatt acagtccgga gaaacgcctt tttccttggt gttccggaag ggtattttcg
     9001 caaatcagga agtgaactac tctttgcact attgcgagaa tactcttgtt tactgcttac
     9061 ctgtttatta ctagctttag ttaagtggga cttttgactc gctgttgttg tgtgcgtctt
     9121 cgctaacacc tcactcgaaa acaccagagt gagtaacata agaatcatcg ccccatatcg
     9181 tcgtatggga gtcaaaatca tcaggtctcc tggtcggatt taatcattcc aacaccttat
     9241 atttttcaca aatttgagag ttgaatctca aatcatatca aaaatagctg tcaagagcac
     9301 cccaaggaat agtccaaatc tgaaactatg tcacgagtta acgattcaga ttggcgctaa
     9361 atcgcagaaa atgtggggtt tatcgcaaaa ttcagccgtt ttttgtgcgc gatcgctcac
     9421 ccttgcttct catcctgtgg acttaccgct cagggatgag ttttgtctgg cttatcgctg
     9481 gcaaactgtc tgaaatcgca gcaataagga ctcatccgcc atgaaactcg ccgcctgttt
     9541 tctgacactc cttcctggct tcgccgttgc cgccagctgg acttctccgg ggtttcccac
     9601 ctttagcgaa caggggacgg gaacatttgt cagccacgcg cagttgccca aaggtacgcg
     9661 tccactaacg ctaaattttg accaacagtg ctggcagcct gcggatgcga taaaactcaa
     9721 tcagatgctt tccctgcaac cttgtagcaa cacgccgcct caatggcgat tgttcaggga
     9781 cggcgaatat acgctgcaac tagacacccg ctccggtaca ccaacattga tgatttccat
     9841 ccagaacgcc gtcgaaccgg tagcaagcct ggtccgtgaa tgcccgaaat gggatggatt
     9901 accgctcaca ctggatgtca gcgccacttt tccggaggga gccgccgtcc gggattatta
     9961 cagccagcaa attgcgatag tgaagaacgg tcaaataacg ttacaacccg ctgccaccag
    10021 caacggttta ctcctgctgg aacgggcaga aactgacgcc cctgcccctt tcgactggca
    10081 taacgccacg gtttactttg tgctgacaga tcgtttcgaa aacggcgatc ccagtaatga
    10141 ccagagttac ggacgtcata aagacggtat ggcagaaatt ggcacttttc acggcggcga
    10201 tttacgcggc ctgaccaaca aactggatta cctccagcag ctgggcgtca atgctttatg
    10261 gataagtgcc ccatttgagc aaattcacgg ctgggtcggc ggcggtacaa aaggcgattt
    10321 cccgcattat gcctaccacg gttattacac acaggactgg acgaatcttg atgccaatat
    10381 gggcagtgaa gccgatctac ggacactggt tgatagcgcg catcagcgcg gtattcgtat
    10441 tctctttgat gtcgtgatga accacaccgg ctatgccaca ctggcggata tgcaggagta
    10501 tcagtttggc gcgttatatc tttctggtga cgaagtgaaa aaaacgttgg gtgaacgctg
    10561 gagcgactgg aaacctgccg ccgggcaaac ctggcatagc tttaacgatt acattaattt
    10621 cagcgacaaa acaggctggg ataaatggtg gggaaaaaac tggatcagaa ccgatatcgg
    10681 cgattacgac aatcctggat ttgacgatct cactatgtcg ctggcctttt tgccggatat
    10741 caaaaccgaa tcaacgaccg cttctggtct gccggtgttc tataaaaaca aaacggatac
    10801 ccacgctaaa gtcatcgacg gctttacccc tcgcgattac ttaacccact ggttaagtca
    10861 gtgggttcgc gactatggga ttgatggttt tcgggtcgac accgccaaac atgttgagtt
    10921 gcccgcctgg cagcaactga aaaccgaagc cagcgccgcg cttgtcgaat ggaaaaaagc
    10981 gaatcccgat aaagcgttag atgacaaacc gttctggatg accggtgaag cctggggcca
    11041 cggcgtgatg caaagtgact actatcgcca cggcttcgat gcgatgatca atttcgatta
    11101 tcaggagcag gcggctaaag cagtcgattg tctggcgcag atggatacga cctggcagca
    11161 aatggcggag aaattgcagg atttcaacgt gttgagctac ctctcgtcgc atgatacccg
    11221 cctgttccgt gaagggggcg acaaagcagc agagttatta ttagcgccag gcgcggtaca
    11281 aatcttttat ggtgatgaat cctcgcgtcc gttcggcccc accggttcgg atccactgca
    11341 aggcacgcgt tcggatatga actggcagga tgttagcggc aaatctgccg ccagcgtcgc
    11401 gcactggcag aaaatcagcc agttccgcgc ccgccatccc gcaattggcg cgggcaaaca
    11461 aacgacactt tcgctgaagc agggctacgg ctttgttcgt gagcatggcg acgataaagt
    11521 gctggtcatc tgggctgggc aacagtaact tttccggctt cccgttcgtc agtacctcgg
    11581 gaagccgcca accaggataa aatgtcagac ctaatcagcg ttgcaggata aagcaccgct
    11641 ccctcttcaa cagagcgatt tgcaccccag caaatgtagc gttattgtta ccttccttgc
    11701 tacagagttc gacagatatc ccgctatgac attctccctt tttggtgaca aatttacccg
    11761 ccactccggc attacgctgc tgatggaaga tctgaacgac ggtttacgca cgcctggcgc
    11821 gattatgctc ggcggcggta atccagcgca gatcccggaa atgcaggact acttccagac
    11881 gctactgacc gacatgctgg aaagtggcaa agcgactgat gcactgtgca attacgacgg
    11941 tccacagggg aaaacggagc tactcacact gcttgccgga atgctacgcg agaagttggg
    12001 ttgggatatc gaaccacaga atattgcact aacaaacggc agccagagcg cgtttttcta
    12061 cttatttaat ctgttcgccg gacgccgtgc cgatggtcgg atcaaaaaag tgctgtttcc
    12121 gctggcaccg gaatacattg gctacgctga cgccggactg gaagaagatc tgtttgtctc
    12181 tgcgcgtccg aatattgagc tgctgccgga aggccagttt aaataccacg tcgattttga
    12241 gcatctgcat attggcgaag aaaccgggat gatttgcgtc tcccggccga cgaacccaac
    12301 aggcaatgtg attactgacg aagagttgct gaagcttgac gcgctggcga atcaacacgg
    12361 cattccgctg gtgattgata acgcttatgg cgtcccgttc ccgggtatca tcttcagcga
    12421 agcgcgcccg ctgtggaatc cgaatatcgt actgtgcatg agtctttcca agctgggtct
    12481 accaggctcc cgctgcggca ttatcatcgc caatgaaaaa atcatcaccg ccatcaccaa
    12541 tatgaacggc attatcagcc tggcacctgg cggtattggt ccggcgatga tgagtgaaat
    12601 gattaagcgt aacgatctgc tgcgcctgtc tgaaacagtc atcaaaccgt tttactacca
    12661 gcgtgttcag gaaactatcg ctatcattcg ccgctattta ccggaagatc gctgcctgat
    12721 tcataaaccg gaaggagcca ttttcctctg gctatggttt aaggatttgc ccattacgac
    12781 cgagcagctc taccagcgcc tgaaagcacg gggcgtgctg atggtgccgg ggcacaactt
    12841 cttcccaggg ctggataaac cgtggccaca tacacatcaa tgtatgcgca tgaactacgt
    12901 gccggagccg gagaaaattg aggcgggtgt gaagattctg gcagaagaga tagaaagagc
    12961 ctgggctgaa agtcactaaa aattgcctga tgcgctacgc ttatcaggcc tacatgattt
    13021 ctgcaatata ttgaatttgg tagaatttgt aggccggata aggcgtttta gccgcatccg
    13081 gcataaacaa agcgcactat ccctgcgttg agaaacgccg gaaaaacgtt tcaaacaggc
    13141 tgctgccgta gccgatgtcg ctgcactttc tcgacatcca tgcactgcaa cgcctttgtc
    13201 gggcaggctt caacacaggc cggtcccgtc gcccgatgcc agcacagatc gcacttaatc
    13261 gccctcgcct ttttgcgcga agaaacgacc tccatcgcac caaacgggca agccagcata
    13321 cagcttttac agccaatgca acgtgattgt tcaacgaaaa tatgcccatg ttcgcggctt
    13381 atcgcgtcaa cagggcagac attcgcgcac ggtgcatctt cacactgatg gcaggctaca
    13441 gccgtggtcc atgtgtggtc tttaatgaca cgaatacggg aaataaactc gtctggtgac
    13501 aacgcagcgc aatcctgatt ctcctgatgc gacactgcgc aagccacttc acaggtacgg
    13561 caaccgatac atttcgtcgc atccgcaata ataaaccggt tcatgatcac tcctttgcac
    13621 taaaacagca gagtgccaga gagcgcgtct ctactgcttt gatccaacga aggaaatgtt
    13681 aaagttttgg cacttgtcca aagcgttatg tgatcgcgcc ctggtcgtcc ctgacagtaa
    13741 atcccagttc attagaaata gcctgcgcgg tttcacgcag tggtttcagg agatttttct
    13801 cccccacctg tttcagacgt gatgtcgaaa gcgaaatcga cacggcgtac ggcacccgcc
    13861 catgaatatc aaacaccgga acagcaatac aggagacgcc gagttcgttt tcttctctgt
    13921 ccatcgccgc tccgctttca cgaatgtgcg ccagttcgtc gaacatcgcg ggcagctcgg
    13981 taatggtatt gcgggttaac ggctggatct catgctgatg gctttcccag tatgacttca
    14041 cgtagtccgg gtggccaaac gccatgtaga tcttgcccat tgcggaacag tagagcggca
    14101 tatgctggcc aatataggca cgggttcgca gcatcccggt tgtgggttcc agcttataaa
    14161 tcaaaatagc gtgatcgtct tcgcggctgg agaagttaat agtttcacca gtggcgatgt
    14221 tcagtgcctc aagatgcgga gcggcgatat gaatgatatt cagcgaagac agcgccttct
    14281 gcccgacggc aataaatttg gtggtcaggc gataactccc tgcggcgggc gcggtggtca
    14341 catagccgca ggattgtaat ccctgcaata agcgatggac ggtactctta tttaaaccag
    14401 ccagctccga aagatgcgcc aacggacaac cgtttggata gttgctcaaa atctcaatca
    14461 gcatcaaccc gcgaaacaga ctctggcttc cggctggacg ctctttttcc tgcgccatct
    14521 cgttctcttt ttttcccatc acttctttcc ccattttgtc gcgtcaagat ggtagcgcaa
    14581 agtgtgtcgt agttcacgat ctcgacagat aatttataac cacttgattt ttaagttttt
    14641 tgaaattcat caatcagatt gctttgttaa aaagcgatcg atatatttga aatcagattt
    14701 cgcatattga aattttaagc caaaaaagcg atcaaaaaaa caaggaagcc agggatgaaa
    14761 gtgacatttg agcagttaaa agcagccttt aatcgagtct taatttcacg cgacgttgac
    14821 agcgaaacgg ctgatgcctg tgccgaaatg ttcgcccgca ccaccgaatc cggcgtttat
    14881 tctcacggcg tcaaccgttt ccctcgtttc attcaacaac tggaaaacgg cgatatcatt
    14941 cctgatgccc aacccaaacg tataaccagc ctcggcgcaa ttgaacagtg ggacgcccag
    15001 cgttcgatcg gtaacctgac agcgaaaaag atgatggatc gcgccattga actggctgcc
    15061 gatcacggta ttggtctggt ggcactacgt aatgccaacc actggatgcg cggcggcagc
    15121 tacggctggc aggcggcgga aaaaggctat attggcattt gctggaccaa ctccatcgcc
    15181 gtaatgccgc cgtggggcgc aaaagagtgt cgcatcggca ctaacccgct gatcgtcgcc
    15241 attccttcta ccccaatcac catggtcgat atgtcgatgt cgatgttctc ttacggcatg
    15301 ttagaagtta accgtctggc aggccgtcag ctcccggtcg atggtggctt tgatgatgag
    15361 ggcaatttga ccaaagaacc tggcgttatc gagaagaatc gccgcatttt gccgatgggt
    15421 tactggaaag gttcgggcat gtcgattgtg ctggatatga tcgccactct cctttctgac
    15481 ggcgcatcgg ttgccgaagt cacccaggac aacagcgacg aatacggcat ttcacaaatt
    15541 tttattgcca ttgaagtaga taagcttatc gacggcccca cccgcgatgc caaactgcaa
    15601 cgcatcatgg attacgttac caccgctgaa cgcgctgacg aaaatcaggc cattcgctta
    15661 cccggccacg aatttactac cctgctggcc gaaaaccgcc gtaacggtat taccgttgat
    15721 gacagcgtgt gggcaaaaat ccaagcgtta taaggagata acccatgatt tttggacata
    15781 tcgcgcagcc tgacccgtgc cgtttgcctg ccgccattga aaaggcgctt gattttctgc
    15841 gcaccaccga cttcaacgcc ctggaaccgg gcgttgtcga aatcgacggc aagaatattt
    15901 atgcgcaaat tatcgattta accacacgcg aagcggcgga aaaccgtccg gaagtgcatc
    15961 gtcggtatat cgatattcag tttctggcgt ggggtgaaga gaaaatcggc attgctattg
    16021 atacgggaaa taataaagtc agcgaatcac tattagagca gcgcgatatt attttttatc
    16081 acgatagtga acatgaatca tttatagaaa tgataccggg cagctacgct attttcttcc
    16141 cgcaggatgt tcaccgacca ggttgtattc tgcaaacaac ctctgagata cgaaaaattg
    16201 tggttaaagt cgcattaaca gcgctgaatt aatcgaataa tcgtctacgc cagaacgcct
    16261 gggtaatgtt attgctctaa ctatattaat aatttaggtt gctgcacatg caacttgaat
    16321 tatgacagac atagcctcaa ggaatagcta tgaaaaaaat acttgaagca atac
//
LOCUS       DAZFLD010000111        16078 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.112, whole genome shotgun sequence.
ACCESSION   DAZFLD010000111 DAZFLD010000000
VERSION     DAZFLD010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16078)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16078)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16078
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.112"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            289..1188
                     /gene="yegS"
                     /locus_tag="ACHKBL_002964"
     CDS             289..1188
                     /gene="yegS"
                     /locus_tag="ACHKBL_002964"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase YegS"
                     /protein_id="HFO5819810.1"
                     /translation="MAEFPASLLILNGKSTDNLPLREAIMLLREEGMTIHVRVTWEKG
                     DAARYVEEARKLGVATVIAGGGDGTINEVSTALIQCEGDDIPALGILPLGTANDFATS
                     VGIPEALDKALKLAIAGNAIAIDMAQVNKQTCFINMATGGFGTRITTETPEKLKAALG
                     GVSYIIHGLMRMDTLQPDRCEIRGENFHWQGDALVIGIGNGRQAGGGQQLCPNALIND
                     GLLQLRIFTGDEIIPTLVSTLKSDEDNPNIIEGASSWFDIQAPHEITFNLDGEPLSGQ
                     NFHIEILPAALRCRLPPDCPLLR"
     gene            complement(1270..2043)
                     /locus_tag="ACHKBL_002965"
     CDS             complement(1270..2043)
                     /locus_tag="ACHKBL_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFO5819811.1"
                     /translation="MNSFERRNKIIQLVNEQGTVLVQDLAGVFAASEATIRADLRFLE
                     QKGVVTRFHGGAAKIMSGNSETETQEVGFKERFQLASAPKNRIAQAAVKMIHEGMTVI
                     LDSGSTTMLIAEGLMTAKNITVITNSLPAAFALSENKDITLVVCGGTVRHKTRSMHGS
                     IAERSLQDINADLMFVGADGIDAVNGITTFNEGYSVSGAMVTAANKVIAVLDSSKFNR
                     RGFNQVLPIEKIDIIITDDAVSEVDKLALQKTRVKLITV"
     gene            complement(2149..3189)
                     /gene="gatD"
                     /locus_tag="ACHKBL_002966"
     CDS             complement(2149..3189)
                     /gene="gatD"
                     /locus_tag="ACHKBL_002966"
                     /EC_number="1.1.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFO5819812.1"
                     /translation="MKSVVNDTDGIVRVAESVIPEIKHQDEVRVKIASSGLCGSDLPR
                     IFKNGAHYYPITLGHEFSGYIDAVGSGVDDLHPGDVVACVPLLPCFTCPECLKGFYSQ
                     CAKYDFIGSRRDGGFAEYIVVKRNNVFALPADMPIEDGAFIEPITVGLHAFHLAQGCE
                     NKNVIIIGAGTIGLLAIQCAVALGAKSVTAIDISSEKLALAKSFGAMQTFNSREMSAP
                     QIQGVLRELRFNQLILETAGVPQTVELAVEIAGPHAQLALVGTLHQDLHLTSATFGKI
                     LRKELTVIGSWMNYSSPWPGQEWETASRLLTERKLSLEPLIAHRGSFESFAQAVRDIA
                     RNAMPGKVLLIP"
     gene            complement(3237..4592)
                     /locus_tag="ACHKBL_002967"
     CDS             complement(3237..4592)
                     /locus_tag="ACHKBL_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015577 - galactitol transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015796 - galactitol transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HFO5819813.1"
                     /translation="MFSEVMRYILDLGPTVMLPIVIIIFSKILGMKAGDCFKAGLHIG
                     IGFVGIGLVIGLMLDSIGPAAKAMAENFDLNLHVVDVGWPGSSPMTWASQIALVAIPI
                     AILVNVAMLLTRMTRVVNVDIWNIWHMTFTGALLHLATGSWMIGMAGVVIHAAFVYKL
                     GDWFARDTRNFFELEGIAIPHGTSAYMGPIAVLVDAIIEKIPGVNRIKFSADDIQRKF
                     GPFGEPVTVGFVMGLIIGILAGYDVKGVLQLAVKTAAVMLLMPRVIKPIMDGLTPIAK
                     QARSRLQAKFGGQEFLIGLDPALLLGHTAVVSASLIFIPLTILIAVCVPGNQVLPFGD
                     LATIGFFVAMAVAVHRGNLFRTLISGVIIMSITLWIATQTIGLHTQLAANAGALKAGG
                     MVASMDQGGSPITWLLIQVFSPQNIPGFIIIGAIYLTGIFMTWRRARGFIKQEKAVLA
                     E"
     gene            complement(4596..4880)
                     /gene="gatB"
                     /locus_tag="ACHKBL_002968"
     CDS             complement(4596..4880)
                     /gene="gatB"
                     /locus_tag="ACHKBL_002968"
                     /EC_number="2.7.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HFO5819814.1"
                     /translation="MKRKIIVACGGAVATSTMAAEEIKELCQSHNIPVELIQCRVNEI
                     ETYMDGVHLICTTARVDRSFGDIPLVHGMPFVSGVGIEALQNKILTILQG"
     gene            complement(4911..5363)
                     /gene="gatA"
                     /locus_tag="ACHKBL_002969"
     CDS             complement(4911..5363)
                     /gene="gatA"
                     /locus_tag="ACHKBL_002969"
                     /EC_number="2.7.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HFO5819815.1"
                     /translation="MTNLFVRSGISFVDRSEVLTHIGNEMLAKGVVHDTWPQALIARE
                     AEFPTGIMLEQHAIAIPHCEAIHAKSSAIYLLRPTNKVHFQQADDDNDVAVSLVIALI
                     VENPQQQLKLLRCLFGKLQQPEIVETLITLPETQLKEYFTKYVLDSDE"
     gene            complement(5373..6635)
                     /gene="gatZ"
                     /locus_tag="ACHKBL_002970"
     CDS             complement(5373..6635)
                     /gene="gatZ"
                     /locus_tag="ACHKBL_002970"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatZ"
                     /protein_id="HFO5819816.1"
                     /translation="MKTLIARHKAGEHIGICSVCSAHPLVIEAALAFDRNSTRKVLIE
                     ATSNQVNQFGGYTGMTPADFREFVFAIADKVGFARERIILGGDHLGPNCWQQENVDAA
                     MEKSVELVKAYVRAGFSKIHLDASMSCAGDPIPLAPETVAERAAVLCFAAESVATDCQ
                     REQLSYVIGTEVPVPGGEASAIQSVHITQVEDAANTLRTHQKAFIARGLAEALTRVIA
                     IVVQPGVEFDHSNIIHYQAQEAQALAQWIEKTKMVYEAHSTDYQTQTAYRELVRDHFA
                     ILKVGPALTFALREAIFALAQIEQELIAPENRSGCLAVIEEVMFDEPQYWKKYYRTGF
                     NDSLLDIRYSLSDRIRYYWPHSRIKNSVETMMVNLQGMEIPLGMISQYLPKQFERIQS
                     GELSAIPHQLIMDKIYDVLRAYRYGCAE"
     gene            complement(6664..7518)
                     /gene="gatY"
                     /locus_tag="ACHKBL_002971"
     CDS             complement(6664..7518)
                     /gene="gatY"
                     /locus_tag="ACHKBL_002971"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416599.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HFO5819817.1"
                     /translation="MYVVSTKQMLNNAQRGGYAVPAFNIHNLETMQVVVETAANLHAP
                     VIIAGTPGTFTHAGTENLLALVSAMAKQYHHPLAIHLDHHTKFDDIAQKVRSGVRSVM
                     IDASHLPFAQNISRVKEVVDFCHRFDVSVEAELGQLGGQEDDVQVNEADALYTNPVQA
                     REFAEATGIDSLAVAIGTAHGMYASAPALDFSRLENIRQWVNLPLVLHGASGLSTKDI
                     QQTIKLGICKINVATELKNAFSQALKNYLTEHPEATDPRDYLQSAKSAMRDVVSKVIA
                     DCGCEGRA"
     gene            complement(7747..8799)
                     /gene="fbaB"
                     /locus_tag="ACHKBL_002972"
     CDS             complement(7747..8799)
                     /gene="fbaB"
                     /locus_tag="ACHKBL_002972"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HFO5819818.1"
                     /translation="MTDIAQLLGKDADNLLQHRCMTIPSDQLYLPGHDYVDRVMIDNN
                     RPPAVLRNMQTLYNTGRLAGTGYLSILPVDQGVEHSAGASFAANPLYFDPKNIVELAI
                     EAGCNCVASTYGVLASVSRRYAHRIPFLVKLNHNETLSYPNTYDQTLYASVEQAFNMG
                     AVAVGATIYFGSEESRRQIEEISAAFERAHELGMVTVLWAYLRNSAFKKDGVDYHVSA
                     DLTGQANHLAATIGADIVKQKMAENNGGYKAINYGYTDDRVYSKLTSENPIDLVRYQL
                     ANCYMGRAGLINSGGAAGGETDLSDAVRTAVINKRAGGMGLILGRKAFKKSMADGVKL
                     INAVQDVYLDSKITIA"
     gene            9056..10333
                     /locus_tag="ACHKBL_002973"
     CDS             9056..10333
                     /locus_tag="ACHKBL_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015858 - nucleoside transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease"
                     /protein_id="HFO5819819.1"
                     /translation="MKTTAKLSFMMFVEWFIWGAWFVPLWLWLSKSGFSAGEIGWSYA
                     CTAIAAILSPILVGSITDRFFSAQKVLAVLMFAGAALMYFAAQQTTFAGFFPLLLAYS
                     LTYMPTIALTNSIAFANVPDVERDFPRIRVMGTIGWIASGLACGFLPQMLGYADISPT
                     NIPLLITAGSSALLGVFAFFLPDTPPKSTGKMDIKVMLGLDALILLRDKNFLVFFFCS
                     FLFAMPLAFYYIFANGYLTEVGMKNATGWMTLGQFSEIFFMLALPFFTKRFGIKKVLL
                     LGLVTAAIRYGFFIYGSADEYFTYALLFLGILLHGVSYDFYYVTAYIYVDKKAPVHMR
                     TAAQGLITLCCQGFGSLLGYRLGGVMMEKMFAYQEPVNGLTFNWSGMWTFGAVMIAII
                     AVLFMIFFRESDNEITAIKVDDRDIALTQGEVK"
     gene            10330..11334
                     /locus_tag="ACHKBL_002974"
     CDS             10330..11334
                     /locus_tag="ACHKBL_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HFO5819820.1"
                     /translation="MKTERILGALYGQALGDAMGMPSELWPRSRVKAYFGWIDRFLPG
                     PKENNAACYFNRAEFTDDTSMALCLADALLEREGKIDPDLIGRNILDWALRFDAFNKN
                     VLGPTSKIALNAIRDGKPVAELENNGVTNGAAMRVSPLGCLLPARDVDSFIDDVALAS
                     SPTHKSDLAVAGAVVIAWAISRAIDGESWSAIVDSLPSIARHAQQKRITTFSASLAAR
                     LEIALKIVRNADGTESASEQLYQVVGAGTSTIESVPCAIALVELAQTDPNRCAVLCAN
                     LGGDTDTIGAMATAICGALHGVNAIDPALKAELDAVNQLDFNRYATALAKYRQQREAI
                     "
     gene            11331..12296
                     /locus_tag="ACHKBL_002975"
     CDS             11331..12296
                     /locus_tag="ACHKBL_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HFO5819821.1"
                     /translation="MSGARLHTLLPELATRQPVMVVGAAVIDVIADAYALPWRGCDIE
                     LKQQSVNVGGCALNIAVALKRLGIEAGNALPLGQGVWAEIIRNRMAKEGLISLIDNAE
                     GDNGWCLALVEPDGERTFMSFSGVENQWNRQWLARLTVAPGSLLYFSGYQLASPCGEL
                     LVEWLEELQDVTPFIDFGPRIGDIPDALLARIMACRPLVSLNRQEAEIAAERFALSAE
                     ITTLGKQWQEKFAATLIVRLDKEGAWYFSNDASGCIPAFPTQVVDTIGAGDSHAGGVL
                     AGLASGLPLADAVLLGNAVASWVVGHRGGDCAPTREELLLAHKNV"
     gene            complement(12270..13016)
                     /locus_tag="ACHKBL_002976"
     CDS             complement(12270..13016)
                     /locus_tag="ACHKBL_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFO5819822.1"
                     /translation="MEQAHTQLIAQLNERILAADNTPLYIKFAETVKNAVRSGVLEHG
                     NILPGERDLSQLTGVSRITVRKAMQALEEEGVVTRSRGYGTQINNIFEYSLKEARGFS
                     QQVVLRGKKPDTLWVNKRVVKCPEEVAQQLAVEAGSDVFLLKRIRYVDEEAVSIEESW
                     VPAHLIHDVDAIGISLYDYFRSQHIYPQRTRSRVSARMPDAEFQSHIQLDSKIPVLVI
                     KQVALDQQQRPIEYSISHCRSDLYVFVCEE"
     gene            complement(13068..13886)
                     /locus_tag="ACHKBL_002977"
     CDS             complement(13068..13886)
                     /locus_tag="ACHKBL_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HFO5819823.1"
                     /translation="MQLRITSRKKFTALLCALGLISIVAIYPRQTVNFFYSTAIQIKD
                     YIHFYGYRPVKSFAIRIPASYTIHGIDVSRWQERIDWQRVAKMRDNGIRLQFAFIKAT
                     EGEKLVDPYFSRNWQLSRENGLLRGAYHYFSPSVSASVQARLFLQTVDFSQGDFPAVL
                     DVEERGKLSAKELRKRVSQWLKMVEKRTGKKPIIYSGAVFYHTNLAGYFNEYPWWVAH
                     YYQRRPDNDGMAWRFWQHSDRGQVDGINGPVDFNVFNGTVEELQGFVDGIKETP"
     gene            complement(13951..14751)
                     /gene="thiD"
                     /locus_tag="ACHKBL_002978"
     CDS             complement(13951..14751)
                     /gene="thiD"
                     /locus_tag="ACHKBL_002978"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HFO5819824.1"
                     /translation="MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQ
                     NTRGVQSVYRIEPDFVAAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNV
                     VLDTVMLAKSGDPLLSPSAVATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLE
                     QGRSLLAMGCGAVLMKGGHLDDEQSPDWLFTREGEQRFTAQRIMTKNTHGTGCTLSAA
                     LAALRPRHTNWTDTVQEAKSWLSSALAQADTLEVGHGIGPVHHFHAWW"
     gene            complement(14748..15536)
                     /gene="thiM"
                     /locus_tag="ACHKBL_002979"
     CDS             complement(14748..15536)
                     /gene="thiM"
                     /locus_tag="ACHKBL_002979"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004417 - hydroxyethylthiazole kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HFO5819825.1"
                     /translation="MQVDLLSSAQSAHTLHLFHQHSPLVHCMTNDVVQTFTANTLLAI
                     GASPAMVIETEEASQFAAIASALLINVGTLTQPRAQAMSAAVEQATRSQTPWTLDPVA
                     VGALDYRRRFCVELLSHKPTAIRGNASEIMALAGVANGGRGVDTTDAAANAIPAAQTL
                     ARETGAIVVVTGEVDYVTDGHRIIGIHGGDPLMTKVVGTGCALSAVVAACCALPGDTL
                     ENIASACHWMKQAGERAVASSEGPGSFVPHFLDALWQLTQEVQA"
     regulatory      complement(15571..15667)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(15759..16031)
                     /gene="rcnR"
                     /locus_tag="ACHKBL_002980"
     CDS             complement(15759..16031)
                     /gene="rcnR"
                     /locus_tag="ACHKBL_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II)-binding transcriptional repressor
                     RcnR"
                     /protein_id="HFO5819826.1"
                     /translation="MSHTIRDKQKLKARASKIQGQVVALKKMLDEPHECAAVLQQIAA
                     IRGAVNGLMREVIKGHLTEHIVHQGDELKREEDLDVVLKVLDSYIK"
BASE COUNT         3942 a         4199 c         3881 g         4056 t
ORIGIN      
        1 acatacacgg atgcccttcc aggtaacccc atgggggggt taatatattt ttaatagtat
       61 attgaagatg ccactgttta gttgaatatt aggtatatgt tcttttttga aatttaccgg
      121 tggctaccgt taatattcgc tgttcccatt gcaagctcct ggtggtaacc actgaatcct
      181 ccatacttga actgactttt tatcctccga ctttcatcct gttctgactc caccttttct
      241 tttctgctct acactatcta cagtccaatc ataaaggcac atacgatcat ggcagaattt
      301 cccgccagct tactgattct taatggcaaa agtactgaca atctaccctt gcgcgaagca
      361 attatgctgt tgcgtgagga aggaatgacg atccatgtgc gggtcacctg ggagaaaggc
      421 gatgccgcac gatatgtaga ggaggcacgg aagttgggcg tcgcaacggt gattgccggt
      481 ggtggcgatg gcaccattaa tgaagtttct acggcgttga ttcagtgtga gggggatgac
      541 atacccgcgc tgggaatttt accattagga accgccaatg attttgccac cagtgtaggg
      601 attcctgagg cactggataa ggcgctgaaa ctggcaattg ccggtaacgc cattgcgata
      661 gatatggcgc aggtcaataa acaaacctgt tttattaata tggcgacagg cgggtttggg
      721 acgcgtatta ccacagaaac gccggaaaaa ttaaaagccg cgctgggtgg cgtctcttac
      781 atcattcatg gcttaatgcg catggacact ctgcaaccgg accgttgtga aatccgcggt
      841 gaaaactttc actggcaagg tgacgccctg gtcattggta ttggtaacgg gcgtcaggcc
      901 ggtggcggtc aacaattgtg cccgaacgcg ttaattaacg atggcttgct gcaactgcgc
      961 atttttaccg gcgatgaaat tattccgact ctcgtatcaa ccttaaaatc tgacgaagat
     1021 aacccgaata ttatcgaagg agcttcgtcg tggtttgata tacaagcccc acacgaaatc
     1081 acttttaatc ttgatggcga accgttgagt gggcaaaact tccatattga aatacttccg
     1141 gcggcgttgc gttgtcgatt accaccagat tgtccattgt tgcgttaatc aaactattct
     1201 ctgttgaacc agagagaatc cgtaatgccg gatgcggtgt aaacacctta tccggcatac
     1261 agaacaatac tacaccgtaa ttaattttac ccgtgtcttc tgcaatgcca gtttatccac
     1321 ctcagacaca gcatcatcgg taataataat gtcaattttt tcgattggca gtacctgatt
     1381 aaaaccgcga cgattaaatt tcgatgaatc gagaacggca atcactttat tcgccgctgt
     1441 caccatcgca ccgctaacgg aataaccttc attaaaggtc gtaataccat taaccgcatc
     1501 aataccatca gcaccgacaa acattaaatc ggcattaata tcctgcaatg aacgctcagc
     1561 aatggaacca tgcatcgagc gcgttttatg gcgcaccgta ccaccacaaa ccaccaaagt
     1621 gatgtcttta ttttcggaaa gggcaaacgc agccgggaga ctgttggtaa tcaccgtgat
     1681 atttttggcg gtcattaatc cttcggcgat aagcattgtc gtacttccgc tgtcgagaat
     1741 aacggtcatc ccttcgtgga tcattttgac tgccgcctgc gctattctgt ttttcggcgc
     1801 gctggcgagc tgaaatcgct ctttaaaccc gacttcctgg gtctcggttt cgctattacc
     1861 ggacattatt ttcgccgcac cgccatgaaa gcgcgtaacc acgccttttt gttcgagaaa
     1921 gcgcaaatcg gcacggattg tcgcttccga ggcagcaaat actcccgcca gatcctgaac
     1981 aagcacggtt ccctgttcat tcactaattg gatgatctta ttccttcgct cgaatgaatt
     2041 catcgtcatt tacccatatt caattgtggc tagtgtaaac gaagggggac cattggtgaa
     2101 tgatctgttt tgcacaatac cgggcctgca tcacgctggc ccgcggtttc agggaatgag
     2161 caacactttg cccggcatgg cattacgagc gatgtcacgc accgcctggg cgaagctttc
     2221 aaagcttcca cggtgagcga ttaatggctc caggcttaac ttacgttctg tcagcaaccg
     2281 gctcgccgtt tcccactcct gccccggcca agggctggag tagttcatcc aactgccgat
     2341 aaccgtcagc tctttacgca atattttgcc aaacgttgca gatgttaaat gcagatcctg
     2401 atgcaacgtg cccaccagcg ccagttgggc atgaggaccg gcaatctcta ccgccagttc
     2461 gacggtttgc gggacgccag ccgtctcgag gataagctga ttaaagcgca gctcgcgtaa
     2521 aacgccctgt atttgcggcg cgctcatttc acggctgtta aatgtttgca tcgctccgaa
     2581 agattttgcc agtgccagtt tttctgagct aatgtcgata gccgtcacac tctttgctcc
     2641 cagcgcgaca gcgcactgaa ttgccagcag gccaatggtt ccggcaccaa taataataac
     2701 gtttttattc tcacatcctt gcgctaaatg gaacgcatgc aggccaacgg taatcggttc
     2761 aataaaagcg ccatcctcaa taggcatatc cgcaggtagt gcaaagacat tgtttcgctt
     2821 aacgacaata tattcggcaa atccaccatc acgccgcgag ccaataaaat catattttgc
     2881 gcactgggaa taaaaccctt tcaaacactc tggacaagta aaacagggta ataacggcac
     2941 acaggcaacc acatcgccag ggtgtaaatc atcaacaccg gatcccaccg catcaatata
     3001 gccgctaaat tcatgaccta acgttattgg ataataatgt gcaccatttt taaatatcct
     3061 gggtaaatcg gaaccacata agcccgagct ggcaattttt acccgcacct catcctgatg
     3121 tttaatttca ggaatgacgc tttctgcaac gcgcacgata ccatcagtat cattcaccac
     3181 tgatttcata aaaactcctg attattaagg gggataacct ccccctcagg taaaaattat
     3241 tctgcgagaa cggctttctc ttgtttaata aagccacgcg ctctacgcca ggtcatgaaa
     3301 ataccggtaa gataaatggc accgataata atgaaaccgg gaatattttg cggggagaaa
     3361 acctgaatca gtaaccaggt aatgggagaa ccgccctgat ccattgaagc caccatgccc
     3421 ccggctttta acgccccagc attagccgcc agttgggtgt gcaaaccaat agtttgcgtc
     3481 gcgatccaca gggtgatgct cataatgatg acacccgaga ttaaggtgcg gaacagattt
     3541 ccacgatgca ccgcgaccgc catcgccaca aagaagccga tggtggcaag gtcgccaaac
     3601 ggcagcacct gattacccgg cacacaaaca gcaattaaaa tggtgagtgg aataaaaatc
     3661 aggcttgccg ataccaccgc cgtatgtccc agcagcaacg ccggatcaag gccaatcagg
     3721 aactcctgac cgccgaactt cgcctgtaaa cgactacggg cctgcttagc gatgggcgtt
     3781 aaaccatcca tgatgggttt aatcacccgt ggcatcagca gcataactgc cgccgttttt
     3841 accgccagct gcaatacacc tttgacatcg taacccgcga ggatgccgat aatcagcccc
     3901 atcacaaaac ccacggtgac cggttcgcca aaagggccaa atttgcgctg aatatcatcg
     3961 gcgctaaatt taattcggtt aacgcctggg attttctcga tgatagcatc gaccagcacc
     4021 gcaatcggcc ccatatacgc cgacgtaccg tgcggaatag caatgccttc cagctcaaag
     4081 aaatttcggg tatcgcgggc aaaccagtcg ccgagcttat aaacaaacgc cgcgtgaatt
     4141 accacacccg ccatccctat catccatgaa ccggttgcca gatgcagcaa cgcgccggtg
     4201 aaggtcatat gccagatatt ccagatatca acatttacca cccgcgtcat acgggtcagt
     4261 aacatcgcca cgttaaccag aatcgcaatc ggaatcgcca ccagcgcaat ttgcgacgcc
     4321 caggtcatgg gtgaagagcc cggccagccg acatcgacca catgcagatt caggtcgaaa
     4381 ttttccgcca tcgctttcgc cgccgggcca atggaatcca gcattaagcc aatcacaagg
     4441 ccaataccaa caaagccaat cccgatatgc agacctgctt taaagcaatc gcctgccttc
     4501 atgcctaata ttttagaaaa aataatgatg acaatcggca gcatcaccgt agggccgagg
     4561 tcgagaatat aacgcatgac ttctgaaaac ataggtcacc cctgtaagat agtcagaatt
     4621 ttattttgta atgcttcgat accgacacca gaaacaaaag gcatgccgtg aactaacgga
     4681 atatcgccaa aacttcgatc caccctggca gtggtgcata tcaaatgcac gccatccata
     4741 taggtttcta tttcattaac ccgacactgg attaattcaa caggaatatt atgactctga
     4801 cacaactctt taatttcttc cgccgccatc gtagaggtcg caaccgcgcc tccgcaagcg
     4861 acaataatct tgcgtttcat aaatagtcct ttttattgtt acagagggat ttattcgtct
     4921 gaatctaaaa catactttgt gaagtattcc tttaactggg tttcaggaag agtgattagt
     4981 gtctcgacga tctcgggctg ttgtaacttg ccaaataaac agcgtaaaag tttcaattgc
     5041 tgctgcggat tttccacaat taacgcaatc accaacgata ccgccacgtc gttatcatca
     5101 tccgcttgct gaaaatgaac tttatttgtt ggccttaaca gataaatggc tgacgactta
     5161 gcatgaatcg cctcacaatg cggtattgca atggcgtgct gctcaagcat tatcccggta
     5221 gggaattctg cttctctggc aattaatgcc tgtggccagg tatcatgaac cacacctttc
     5281 gcgagcatct cattaccgat atgggttaaa acttcgctac gatcgacaaa agaaattccg
     5341 ctacgaacaa acaggttagt catataccgt ccttattccg cacagccgta gcgataggcg
     5401 cgcaaaacat cataaatttt atccataatc aactgatgcg gtattgctga taattccccg
     5461 gactgaatgc gttcaaattg tttaggaaga tactgactaa tcatgcccag agggatttcc
     5521 atgccttgca gattcaccat catcgtttcg acgctatttt taatccggct atgcggccag
     5581 taataacgaa tacgatccga caggctgtaa cgaatatcca gtaatgaatc gttaaaaccc
     5641 gtacgataat attttttcca gtattgcggt tcgtcaaaca tcacttcttc aattaccgcc
     5701 aggcaaccgc tgcgattttc tggggcgata agttcctgct caatttgcgc cagcgcaaat
     5761 atcgcctcac gtaaagcaaa ggttaatgcc ggaccgactt tcaaaattgc aaaatgatcg
     5821 cgaactaatt cccgataggc cgtctgagtc tggtaatcgg tggaatgcgc ttcataaacc
     5881 attttggttt tttctatcca ttgcgccagc gcctgagctt cttgcgcctg ataatgaata
     5941 atattgctat ggtcaaattc cacacccggc tgcaccacga tggcaatcac gcgtgttaac
     6001 gcctctgcca gaccgcgggc aataaacgct ttttgatgcg tgcgtaaagt attggcggca
     6061 tcttcaacct gggtgatatg tactgactga atggcgctgg cctcaccgcc cggaaccgga
     6121 acttcggtgc caatgacata gctcagttgc tcacgctggc aatctgtcgc cacactttcc
     6181 gcagcaaagc aaagcacagc agctcgttcc gcaaccgttt ctggggctaa cggtatagga
     6241 tcccccgcgc aagacattga cgcatcaaga tgaattttac tgaagccggc acgaacatat
     6301 gcctttacca gttcgacgga tttttccatc gccgcatcca cattttcttg ctgccagcag
     6361 tttggtccca gatggtcgcc accgagaata atacgctcac gagcaaatcc gactttatcg
     6421 gcaatcgcaa aaacaaattc gcgaaagtct gccggtgtca ttccggtata accgccaaat
     6481 tgattgacct ggtttgacgt tgcttcaatc agcactttac gcgtgctgtt gcgatcaaat
     6541 gccagcgccg cttcgataac caacggatgt gcagaacaga ctgaacatat gccaatatgt
     6601 tcaccagctt tatgccgggc aattaacgtt ttcatgtttt ttccttgtta aatggcaggt
     6661 gcgttatgcc ctgccctcgc agccacaatc ggcaatcact ttgctcacca catcgcgcat
     6721 tgcggattta gccgactgca aataatcccg gggatcggtc gcttcggggt gctctgtcag
     6781 gtaatttttt aacgcctgcg agaaggcatt ttttagctct gttgcaacgt tgattttgca
     6841 tatccccagt ttgatggttt gctgaatatc cttagttgat aatcctgacg cgccatgcag
     6901 caccagcggt aagttcaccc actggcgaat gttctccagt cgagaaaaat caagcgccgg
     6961 tgcgctggca tacataccgt gagccgtgcc gatggcgacc gccagggaat caattccggt
     7021 tgcctcggca aattcacgcg cctgaacagg gttggtgtac aacgcatcgg cttcattgac
     7081 ttgcacatca tcttcctggc cgccaagttg ccccagctcc gcttcgacgc tgacatcaaa
     7141 gcgatggcaa aaatccacca cctctttgac ccgtgaaata ttttgcgcaa aaggcaaatg
     7201 cgaggcgtca atcatgactg agcgcacgcc agaacgaacc ttctgagcga tatcgtcaaa
     7261 tttcgtgtga tggtcgagat gaattgccag tggatggtga tattgcttcg ccatcgcgct
     7321 gaccagcgcc aacagatttt ctgtaccggc atgagtaaat gtgccaggcg ttccggcgat
     7381 gatgaccggc gcatgcaggt tggcagcggt ttctaccacc acttgcatcg tttcgagatt
     7441 gtgaatattg aatgccggaa ccgcataacc gccgcgctgt gcgttgttca gcatctgctt
     7501 tgtcgatacc acgtacattt tcatatcctg tcgtttgttt tcgatttcaa aatataatga
     7561 aatgatttgt ttttaaatgt cgctataacg atcacaaaaa cgacaatatg aaaatcaatc
     7621 caggaatgaa aggcaaaaaa aacggcctcc cgggggaagc cgtaacaaag tacgcgtggt
     7681 tttatgccgg atgtggcgtt aacgcctttt ccggcctacg gggtagtgct gttcgtatga
     7741 agcgcatcag gcgatagtaa ttttgctatc gagataaacg tcctgcacgg cgttaatcag
     7801 tttcacgccg tcagccatcg atttcttgaa cgccttacgt ccaagaatca gccccattcc
     7861 gcctgcgcgt ttgttgataa ccgcagtacg cactgcatcg ctgaggtcag tttcaccgcc
     7921 tgcagcaccg ccggagttta tcaacccggc ccggcccata tagcagttag ctaactgata
     7981 acgcaccagg tcaatcgggt tttcactggt cagtttgctg tagactcgat cgtcggtata
     8041 accgtaatta atcgctttat agccgccgtt attttccgcc attttttgtt tgacgatatc
     8101 tgcacctatg gttgccgcca gatggttcgc ctgaccagtc aggtcggcgg aaacatggta
     8161 atcaacgcca tctttcttaa aggcggagtt acgcaaatag gcccacagca ctgtcaccat
     8221 gcccagctcg tgcgcacgtt caaaagccgc agaaatttct tcaatctggc gacgtgactc
     8281 ttccgagcca aaatagatag tcgcaccaac cgccaccgcg cccatattga acgcctgctc
     8341 cacgctggca tacagcgttt gatcgtaggt attcgggtaa cttagcgtct cgttgtgatt
     8401 aagtttgacg aggaatggaa tgcgatgcgc gtaacgccgc gataccgatg ccagcacgcc
     8461 gtaagttgac gccacacagt tacagcccgc ttcgatcgcc agttcaacaa tgtttttcgg
     8521 gtcaaagtag agcgggttag cggcaaatga agctccggca gagtgctcaa cgccctggtc
     8581 aaccggcaga atagaaagat atcctgtgcc agccagacgc ccggtgttgt acaacgtctg
     8641 catattacgt aacaccgctg gcgggcgatt attgtcaatc atcacgcggt ctacgtagtc
     8701 atgtccgggg agataaagct ggtcagaagg aatagtcata caacggtgct gtaaaaggtt
     8761 gtcggcgtct ttgccaagca actgcgcaat atctgtcatt actatgctcc cgtaaattcc
     8821 gattggatat cggctatgga ttttcctggc ccgccttttg cgggcaatca taatcctggt
     8881 cgttacaggc gagtttttcc atcttttgga tgttttttca gcgttttctg ttggcacgat
     8941 tcatcagaaa aaatgttatc gcggtcaagt tttcaccgca aaggtattta aaaggtatta
     9001 ttaaatggta ttgtcatcgc gtaccttaca ttacctgtca tgaaggaatt aaaagatgaa
     9061 aacaacagca aagctgtcgt tcatgatgtt tgttgaatgg tttatctggg gcgcgtggtt
     9121 tgtgccattg tggttgtggt taagtaaaag cggttttagt gccggagaaa ttggctggtc
     9181 gtatgcctgt accgccattg cggcgatcct gtcgccaatt ctggttggct ccatcactga
     9241 tcgctttttc tcggcgcaga aagtgctggc ggtattgatg ttcgctggtg cggcgctgat
     9301 gtatttcgct gcgcaacaga ccacttttgc cgggttcttc ccgttactgc tggcctactc
     9361 gctaacctat atgccgacca ttgcgctgac taacagtatc gcttttgcca acgtgccgga
     9421 tgtggagcgt gatttcccac gcattcgtgt gatgggcact atcggctgga ttgcctccgg
     9481 tctggcatgt ggatttttgc cgcaaatgct gggttatgcc gatatctctc cgactaacat
     9541 cccgctgctg attaccgccg gaagttctgc tctgctcggt gtgtttgcgt ttttcctgcc
     9601 cgacacgccg ccaaaaagca ccggcaaaat ggacattaaa gtcatgctcg gcctggatgc
     9661 gctgatcctg ctgcgcgata aaaacttcct cgtctttttc ttctgttcat tcctgtttgc
     9721 gatgccactg gcgttctatt acatctttgc caacggttat ctgacagaag tcggcatgaa
     9781 aaacgccacc ggctggatga cgctcggcca gttctctgaa atcttcttta tgctggcatt
     9841 gccgtttttc actaaacgct ttggtatcaa aaaggtattg ttgcttggtc tggtcaccgc
     9901 tgcgatccgc tatggcttct ttatttacgg tagtgcggat gaatatttca cctacgcgtt
     9961 actgttcctc ggcattttgc tgcacggcgt cagttacgat ttttactacg ttaccgctta
    10021 catctatgtc gataaaaaag cccccgtgca tatgcgtacc gccgcgcagg ggctgatcac
    10081 gctctgctgc cagggcttcg gcagtttgct cggctatcgt cttggcggtg tgatgatgga
    10141 aaagatgttc gcttatcagg aaccggtaaa cggactgact ttcaactggt ccgggatgtg
    10201 gactttcggc gcggtgatga ttgccattat cgccgtgctg ttcatgattt ttttccgcga
    10261 atccgacaac gaaattacgg ctatcaaggt cgatgatcgc gatattgcgt tgacacaagg
    10321 ggaagttaaa tgaaaacaga acgtattctc ggtgctcttt atgggcaggc gttaggggat
    10381 gcgatgggga tgccctccga gctttggccg cgcagtcgcg tcaaagcgta ctttggctgg
    10441 attgaccgtt ttctgcctgg gccaaaggag aataacgcgg cctgttattt taaccgcgcc
    10501 gaattcaccg acgatacctc gatggcgctg tgtctggcgg atgcgttact ggaacgtgaa
    10561 ggcaagatcg atccggatct gattgggcgt aatattctcg actgggcgct gcgtttcgac
    10621 gcctttaaca aaaacgtact aggtccgacc tcgaagattg ctctcaacgc cattcgcgac
    10681 ggtaaacccg ttgctgaact ggaaaacaac ggcgtgacca acggcgcagc gatgcgcgtc
    10741 tcgccattag gttgtttgct tccggcgcgt gatgttgatt cctttattga tgatgtggcg
    10801 ctggcctcca gcccgacaca taaatccgat ctggcggttg cgggggcggt agtcatcgca
    10861 tgggcgattt ctcgtgccat tgacggagaa agctggtcag cgattgttga ttcactgcct
    10921 tcaattgcgc gacatgctca acaaaaacgc atcactacct tcagcgcctc actggcagca
    10981 cgtctggaga ttgcgctgaa aattgtgcgc aatgccgacg gcaccgaatc cgccagcgaa
    11041 cagctttacc aggtcgttgg cgcaggtacc agcactattg agtccgttcc gtgcgccatt
    11101 gcgctggttg aactggcaca aaccgacccg aaccgttgcg ccgtcctgtg cgctaacctt
    11161 ggcggcgaca cagacaccat cggtgctatg gcgacggcaa tttgtggcgc gttgcatggc
    11221 gttaacgcta tcgatcctgc gttaaaggcg gaactggatg cagtaaatca gcttgatttc
    11281 aaccgctatg ccacggcgct ggcgaagtat cgccaacaac gggaggcgat atgagcggcg
    11341 ctcgattaca cacgctgctg cctgaattag ccacgcgtca gccagtgatg gtcgtcggcg
    11401 cggcggtcat tgatgtgatc gccgacgctt atgccctccc ctggcgtggg tgcgatatcg
    11461 aactgaaaca gcagagcgtt aacgttggcg gctgcgcact gaatattgcc gtggcgttaa
    11521 agcgcctcgg catcgaagcg ggtaatgcct tgccgctcgg tcagggcgtg tgggcggaga
    11581 ttattcgcaa ccggatggca aaagagggct taatcagcct gatcgataac gccgaaggtg
    11641 ataacggctg gtgtctggcg ctggttgagc cggatggcga acgcactttt atgtcattca
    11701 gcggtgtgga aaatcagtgg aatcgccagt ggctggcgcg attaaccgtt gcgcctggca
    11761 gcctgctcta tttttccggt tatcaactgg cctcgccctg cggcgaattg ttagtggaat
    11821 ggctggaaga gctgcaagac gtgacgccat ttatcgattt tggcccacgt attggcgata
    11881 tcccggatgc attactggcg cggatcatgg cctgtcgacc tttagtgtca ctcaatcgtc
    11941 aggaggctga gattgccgcc gaacgttttg ctttatccgc agagataaca acacttggca
    12001 agcaatggca ggagaaattt gctgcgacgt tgatcgttcg tctcgataaa gagggcgcat
    12061 ggtatttcag caacgacgct tctggctgca ttccggcatt tccaacgcaa gttgtagaca
    12121 ccattggggc gggcgacagt catgccggcg gcgtacttgc cgggctggcc tctggtctgc
    12181 cactggcgga tgccgtatta ctgggcaatg cagtggcgtc gtgggttgtc gggcatcggg
    12241 gcggtgattg tgcgccaacg cgcgaggaac tactcctcgc acacaaaaac gtatagatcg
    12301 ctgcgacagt ggctgatgct gtactcaata ggccgttgct gttggtcaag cgccacttgc
    12361 ttgatcacca gcaccggtat tttgctatct aactgaatat gtgactgaaa ctcggcatcc
    12421 ggcatccggg cgctaacgcg ggaacgcgta cgttgtgggt aaatgtgctg gctgcggaag
    12481 tagtcataaa gagaaatccc gatggcatca acatcatgaa ttaaatgtgc cggaacccac
    12541 gattcctcaa tcgataccgc ctcttcatcg acatagcgaa tacgcttaag caaaaagaca
    12601 tcacttcctg cttcgaccgc tagctgctgc gcgacttctt cgggacattt cacgacacgc
    12661 ttgttaaccc atagcgtatc gggctttttc ccgcgcaata ccacctgctg agaaaaacct
    12721 cgcgcttctt tcaacgagta ttcgaagatg ttgttgatct gcgtgccgta accacgcgaa
    12781 cgcgtcacca cgccctcttc ttccagcgcc tgcatcgcct tgcgcaccgt aatgcgcgaa
    12841 acgccggtta actgacttaa atcacgctca ccgggcaaaa tattgccatg ctccagcacc
    12901 ccgctgcgca cggcattttt taccgtttcg gcaaacttaa tataaagcgg cgtgttatcc
    12961 gccgctaaaa tacgttcgtt cagttgggca atcagctggg tatgcgcttg ttccatttgt
    13021 ctttttcctg ccagggagtc tggtgggagt atatcactgc ttcttattta aggcgtttct
    13081 ttaatcccat caacgaatcc ctgcaactcc tccaccgtgc cattaaacac attaaaatcc
    13141 accggaccat taatgccatc tacctgtcca cggtcggaat gctgccagaa gcgccaggcc
    13201 attccgtcat tgtccggacg acgttgatag tagtgagcca cccaccacgg atattcatta
    13261 aaatagcccg ccagattggt gtgataaaaa acggctcctg agtaaataat tggctttttt
    13321 cccgtacgtt tttcgaccat ttttagccac tgacttaccc gcttgcgtaa ttcttttgcc
    13381 gataattttc ctcgctcttc tacgtccagt accgcaggga aatcgccttg tgagaaatcc
    13441 accgtttgca gaaataatcg cgcctgaact gaagcggata ctgacgggga gaaataatga
    13501 tacgccccgc gcagcaggcc attttcgcgg cttagttgcc agttacgcga aaaatagggg
    13561 tccaccagct tttcgccttc cgtcgcctta ataaaagcaa actgtaagcg gataccgttg
    13621 tcgcgcattt ttgccacccg ctgccagtcg atccgctcct gccagcgcga aacatctatt
    13681 ccgtgaatgg tgtaactggc aggaatgcga atagcgaaag atttaaccgg gcgataaccg
    13741 tagaagtgga tgtagtcttt aatctgaatt gctgtcgaat agaaaaaatt caccgtctga
    13801 cgcggataaa ttgcaacgat ggaaattagc ccaagagcgc ataataaagc ggtgaatttt
    13861 tttcgactgg tgattcttag ctgcattggt ttcatccctg aatgtcagcg ccaaaggctg
    13921 acaataacca aagcagctat agtacggtgc tcaccaccag gcgtggaagt ggtgaaccgg
    13981 accaataccg tgaccaactt ccagcgtgtc ggcctgggct aatgccgatg aaagccagct
    14041 ttttgcctcc tgtacggtgt cagtccagtt tgtatggcgc gggcgcagtg cagccaacgc
    14101 cgcagagagc gtacaaccag tgccgtgagt gtttttggtc ataatgcgct gtgcggtaaa
    14161 ccgttgttca ccctcgcggg taaacagcca gtccgggctt tgctcatcat ccagatgacc
    14221 acctttcatt agcactgcgc cacagcccat cgccaacagc gatcgccctt gttccagcat
    14281 ttcctgttcg gtgcgcgcgt gtggcgcgtc gagcaaggcg gctgcttcgg gcaagtttgg
    14341 cgttattaat gaaacctgtg gcaataatcg actgcgcagc gtagcaaccg ccgaaggtga
    14401 aagcagcggg tcgccgcttt ttgccagcat aacggtgtcg agtaccacgt tttggatctg
    14461 ataacgttgc aaccgttctg ccaccgcttc aacaatatcg gtttccgcca acataccgat
    14521 tttagtggtg tcgattcgca catcgctgaa caccgaatcg agctgggcgg cgacaaaatc
    14581 aggctcaatg cgatacaccg actgtacacc acgggtattt tgcgccacca gtgcagtaat
    14641 aactgagcaa ccataagcgc caagtgccga gaaggtttta agatcggcct gaatccccgc
    14701 accaccactc ggatcagtac cggcaatcgt cagagcgtta attcgtttca cgcttgcacc
    14761 tcctgcgtca attgccagag cgcatcaagg aaatgtggaa caaaactgcc tggcccctcg
    14821 ctactggcga ctgcgcgttc tccggcttgt ttcatccagt gacaggcaga tgcgatattt
    14881 tccagcgtat cgcctggtag cgcacaacag gcagcgacaa ccgccgataa tgcacagcca
    14941 gttcctacca ctttggtcat taacggatcg ccaccgtgaa tgccaatgat acgatgtcca
    15001 tcggtaacat aatccacctc gccagtgacc acgacgattg cgccagtttc ccgtgccagt
    15061 gtttgtgcag cgggtatcgc gttagctgcg gcatcagtgg tatccactcc ccgtccacca
    15121 ttagcaacgc cagctaatgc catgatttcc gaagcattac cacgtatggc ggttggctta
    15181 tgggacagaa gttccacaca aaaacggcgg cgataatcga gcgcaccaac cgcaaccgga
    15241 tcaagcgtcc agggcgtttg tgatcgtgtt gcttgctcaa cggcagcact catcgcttgc
    15301 gctcgtggct gcgtcagcgt gccaacgtta atcaacaagg cactggcgat agccgcaaac
    15361 tgactggcct cttcggtttc gataaccatc gctggcgatg caccgatcgc cagcaaggta
    15421 ttggcagtaa aagtttgcac cacatcgttg gtcatgcagt gcacaagagg ggaatgttgg
    15481 tgaaaaaggt gtaacgtgtg cgcagattgc gcggaactca gcaggtcgac ttgcatagtt
    15541 tgctcctgcc ataacgtgaa gaagcaatga cctggtggtc cgtgacttcc ctacgctggc
    15601 attatccaga tcaggtgata cgggtatttc tcagccttca caaagaaggg caccccgagt
    15661 cgtttggttg cgataataaa tcgcagagga ggatggtaat gtccagcgca cgcgttgtaa
    15721 acggggaaaa acggcggtat tacaatcgcg ttaataaatt atttgatata agaatccagc
    15781 accttcagaa cgacatccag atcttcttca cgttttagct catccccctg gtgaacgatg
    15841 tgttccgtca gatggccttt aatcacttcc cgcatcagac cgtttaccgc gccacggata
    15901 gcagcaatct gttgtaaaac tgcagcgcat tcatgcggtt cgtcgagcat tttcttgagc
    15961 gccacgacct ggccctgaat cttactggca cgcgctttca gtttctgttt atcacggatt
    16021 gtatgagaca tggcaacacc tggttaacaa gaatatgaaa aatcatagca ctattaat
//
LOCUS       DAZFLD010000112        16071 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.113, whole genome shotgun sequence.
ACCESSION   DAZFLD010000112 DAZFLD010000000
VERSION     DAZFLD010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16071
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.113"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(5..862)
                     /gene="repA"
                     /locus_tag="ACHKBL_002981"
     CDS             complement(5..862)
                     /gene="repA"
                     /locus_tag="ACHKBL_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HFO5819827.1"
                     /translation="MTDLQQTYYRQVKNPNPVFTPREGAGTLKFCEKLMEKAVGFTSR
                     FDFAVYVAYARSRGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTLAIECG
                     LATESEAGKLSITRATRALKFLSELGLITYQTEYDPTIGCNIPTDITFTPALFDSLDI
                     SEEAVASARRSRVEWENKLRKKQGLDALGMDELIARAWRFVRERFRSYQAELKSHGMK
                     RARARRDAGRTRQDIVTLVKRQLTREIAEGRFRGSLEAVKREIDRRVKERMIMSRNNN
                     YTRLATASP"
     gene            complement(855..929)
                     /gene="tap"
                     /locus_tag="ACHKBL_002982"
     CDS             complement(855..929)
                     /gene="tap"
                     /locus_tag="ACHKBL_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001370046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA];
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepA leader peptide Tap"
                     /protein_id="HFO5819828.1"
                     /translation="MPGKVQYFFLCSLLLRIVSAGWCD"
     gene            complement(1152..1415)
                     /gene="repA"
                     /locus_tag="ACHKBL_002983"
     CDS             complement(1152..1415)
                     /gene="repA"
                     /locus_tag="ACHKBL_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication regulatory protein RepA"
                     /protein_id="HFO5819829.1"
                     /translation="MSDAVRSATSSEARPKRAYRKGAPLSVNERKRLSLNRKRDTHKA
                     LNVFIRNELKDILERFCQDKHLTLGEGIELLIQQEKERAKNGE"
     gene            complement(1819..2085)
                     /locus_tag="ACHKBL_002984"
     CDS             complement(1819..2085)
                     /locus_tag="ACHKBL_002984"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5819830.1"
                     /translation="MDNGKTRTLWGVGLESAVAGYSKGERLRLEDKGTEKVQWIERMK
                     DGREIEKNGDRRVWDCRSLTRESEVMKGFRVSGMPERDDGPDVA"
     gene            complement(2160..2741)
                     /locus_tag="ACHKBL_002985"
     CDS             complement(2160..2741)
                     /locus_tag="ACHKBL_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFO5819831.1"
                     /translation="MALLGYARVSTSHQKLTHQISELKNAGVREDRIFTDMMTGATDE
                     REGLQRLLARAEKDDVIICTKMDRLGRNTADMIRVVDSCYKKGIAIRFLENGLSTEGT
                     MGKMVIQILAAVAEAERERILERTNDGRIAALAAGVKFGRKKHPRTPTALELISQGES
                     LGSVTEKTGISRSTYFRLKRTIKNDAKIATFYK"
     gene            2898..5936
                     /locus_tag="ACHKBL_002986"
     CDS             2898..5936
                     /locus_tag="ACHKBL_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001175011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="HFO5819832.1"
                     /translation="MQKYKKRISILTKSEINELYQVPSFNPVERDEFFSLDISLKREI
                     DKIINIESRVYLILITGYFRYKPVIPEFTLKDVKRDVKYIIQTYYKNESTNLNLNIPK
                     STKYRLVKRMLSTLGFEQLTPSIKNELSERLNDVATICMEPKYIFDELLAFLGQRRIA
                     LPGYSTVQNLVSDALNFERKRTINILSKRIYPATAQKITSIIHDDGILNSIRGYNYST
                     RDFSASEIENELRTHNVISSIYYEIKDIINSLGISQGNMEYYATIVKHSTTFRLKRHP
                     KWQGILYLCCYLYFRYREINDKIITAFRYLVKKHKDASVLFAKARATEEIDLINKKLK
                     FASKIFNFFVDSNIEDSIPFGYVREKVFSLISKDDMHLVSEFFDKNKIDVIEYQWQYI
                     DSHHLKIANSIRKLFLAIDIVYDNEKSVIGQQITAAKDDLRGKGKLDAKDLRVIHPSE
                     KNYIVKEGEINTQRFEFHMYSRVSNHLENGVFYAAESEQNKRLEDDLIERSYWHKNKS
                     ILIEKTGLERLITPITQTLNELENTFSDKLARITIGINSDANEFVKKQPHSNRLQWSL
                     ASKKWKTSIDNPIYNQIKNIGIVDVMKYVNNETNFLSVFNAISSRKNNLEADYNDLLA
                     CIFGNGANYGVHRMASASDRSIGILRDVNDKFIRPETTGMANTVISDAIANLSVYKYW
                     TINEDSPFGSVDGQKHTCRINTFKARYSAKYFRKGKGVSAMTLVSNHVPLSTVVISPN
                     EYEGHFAFDLLYNNTSEIQPKSLATDNHGINNVNFAILDIFGYQFSPRYAKFKKIFNE
                     LFDVILESDKLIIKLKKQFNFKLIEHEWENIQHIICSLSRKTTIQSTVVKKLSNTKRS
                     NKTLAALREYDRVIKCIYVLDYADNKTLRQFVQQALNRGEAYHQLRRAIASVNGNQFR
                     GGNDYQIDQWNDCARLIANCVIYYNSAILSRLVEKFEKENNKKAIDVLANLSPVAWRH
                     ILLGGNYSFEDQVAITNLDSLLEGVDPLSDIGDIESEYDG"
     gene            6002..6199
                     /locus_tag="ACHKBL_002987"
                     /pseudo
     CDS             6002..6199
                     /locus_tag="ACHKBL_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010972061.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            6664..7644
                     /locus_tag="ACHKBL_002988"
     CDS             6664..7644
                     /locus_tag="ACHKBL_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid segregation protein ParM"
                     /protein_id="HFO5819833.1"
                     /translation="MRIFVDDGSTNIKLAWVEDGAVKTLISPNSFKPEWSMTLQGLTD
                     ATIPASANYEIDGEKYSFDQLSPDAVRTTETRYQYSDVNVVAIHHALMQSGIAPQPVD
                     IVVTLPLGEYLDENDQPNLANIERKKNNVRRAVTVQGRENFTIRKVSVLPESVPAGFD
                     VLKDLNDLESLLIVDIGGTTLDIAHVRSKMSGFTKTYCDPKTGVSIITEAVKHAMDVN
                     VSTRTSSYFADRLIQGRDDNEFLTRYIPNAEQREQILTVLCDREKKLQHRVADSVGRF
                     AGFTHVMVVGGGASLVAGAVKKATGVGDNRFFVSDNPQFDLVLGMMAMKG"
     gene            7647..8069
                     /locus_tag="ACHKBL_002989"
     CDS             7647..8069
                     /locus_tag="ACHKBL_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001617754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="HFO5819834.1"
                     /translation="MADNRKCSFYIYPERNAADRVADRFLEKLPQKERGRAMRAMMLC
                     GAALMKQDERLPFLIAEFLTDSTSMQDIQRIISSTLPQQENGEVVRLLEAFLQSAGNN
                     AKTILPAVDSASQEISAPVDQNLLETRNNIKNLFPDDE"
     gene            8093..8383
                     /locus_tag="ACHKBL_002990"
     CDS             8093..8383
                     /locus_tag="ACHKBL_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5819835.1"
                     /translation="MLRPFFFSDKGCRRAPLFTAPADAARQKRCLWSVSRVRRARDGR
                     FYRSRLASVTVYASQSPFSDERPSSRFRGITLPSKRRRLRYSTVGLTRYRTR"
     gene            8493..9395
                     /locus_tag="ACHKBL_002991"
     CDS             8493..9395
                     /locus_tag="ACHKBL_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
                     /protein_id="HFO5819836.1"
                     /translation="MYFSGEPAQIAEIKRLASGAVTPFYRRATNEGIQLFLAGSAGLL
                     QTTEDVQFEPCPGLTAAGRGVVSPENIAFTRWLTHLQNGLLLDEQNCLMLHELWLQSG
                     TGQRRWEGLPDDVRDTITALFTAKRGDWCGFWSNEDVSVWWNRLCDNVVPEKTMPFDL
                     LTVLPTRLDVEVNGFNGGVLNGVPSAYHWYTEQYGVKWPVGYEVNISSQGDNFIQVDF
                     DTPWCQPESDVIAELSRRFSCTLEHWYAEQGCDFCGWQLYERGELVDVLWGELEWSSP
                     TDDDELPEVTGPAWIVDNVAHYGG"
     gene            9359..9454
                     /locus_tag="ACHKBL_002992"
                     /pseudo
     CDS             9359..9454
                     /locus_tag="ACHKBL_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001324028.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            9800..10225
                     /locus_tag="ACHKBL_002993"
     CDS             9800..10225
                     /locus_tag="ACHKBL_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001198918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFO5819837.1"
                     /translation="MQYAKSVTLNVEECDRLSFLPYLFGNDFLYAEAYVYALAQKMMP
                     EYQGGFWHFIRLPDGGGYMMPDGDRFHMVNGANWFDRTVSADAAGVILTSLVINRQLW
                     LYHDSGDAGLTQLYRMRDAQLWRHIEFHPECNAIYAALD"
     gene            10272..10694
                     /locus_tag="ACHKBL_002994"
     CDS             10272..10694
                     /locus_tag="ACHKBL_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
                     /protein_id="HFO5819838.1"
                     /translation="MYCTVKEIIRDVLDTDVPDSECVFAVVLTRGDVRHIAQDWSLTD
                     DELETIMQRLDDAFEHGADVSVVHDVVRELMEEKRASRQVTVPAVMLEKVMALAGSEM
                     KRLYAVGSENGGDGDAFVREEREAMDVVLQALDGENMS"
     gene            10691..10882
                     /locus_tag="ACHKBL_002995"
     CDS             10691..10882
                     /locus_tag="ACHKBL_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5819839.1"
                     /translation="MNISTETREILRNYKAVINARRREMGQKPLTTAQIVDEICDFVA
                     NQQAVFLGGHYILQGSRHR"
     gene            complement(11215..11283)
                     /locus_tag="ACHKBL_002996"
                     /pseudo
     CDS             complement(11215..11283)
                     /locus_tag="ACHKBL_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052655.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pilus protein"
     gene            11775..12089
                     /locus_tag="ACHKBL_002997"
     CDS             11775..12089
                     /locus_tag="ACHKBL_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000412203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5819840.1"
                     /translation="MTQAVISNICAALGNGWMINPSPKQDLIKGYFQLVSAEKKYEHF
                     SMYCFVMNGMLHIYGCVYDELNGDMAWIPLNKSMPDIVRIIKRKVISQKNRLLSIVSY
                     RK"
     gene            12170..12490
                     /locus_tag="ACHKBL_002998"
     CDS             12170..12490
                     /locus_tag="ACHKBL_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000794216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5819841.1"
                     /translation="MNYSEQVAQIILEQLGGKRFIAMTGASNLAYFDDEGESGLCFRL
                     PSHFAINNINIVRIKLTFADDYTVTFYSKQGSRIAQISEHEGIYCDQLQWLFTRETGL
                     YTRL"
     gene            12572..13510
                     /locus_tag="ACHKBL_002999"
     CDS             12572..13510
                     /locus_tag="ACHKBL_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HFO5819842.1"
                     /translation="MSKKTPTTPTPHDGAFRAFLSSPEVARDFVSLHLPAEYRGLCDL
                     STLKIEPCSFVDEELKKYECDALFSMKTPDGEDGYVYCLAEHQSSDNPFMAFRMLRYS
                     VAAMQRHFEQHKRLPLVIPILFYHGERSPYPHSMGWMDCFDKPEIVAKIFTSPFHLVD
                     LTVTDDDEFMHHRRMGALSLMMKHIRSRDLMMQLDRLATLIRETLDERLTLILVNYLL
                     NGSEHVTVEFLQTLAQRLPQHEDSIMTLAERLKQEGIQKGRMEGRMEGRMEGRMEGRL
                     EEKQDIARQLQKMGMTPEQIKLATGLSDDDLKKITH"
     gene            14408..14542
                     /locus_tag="ACHKBL_003000"
     CDS             14408..14542
                     /locus_tag="ACHKBL_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5819843.1"
                     /translation="MSMRQIVTLADAVVKGQPTRIPPMKGDEWREFCWWLDHLRGYAM
                     "
     gene            14607..15086
                     /locus_tag="ACHKBL_003001"
     CDS             14607..15086
                     /locus_tag="ACHKBL_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000154081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5819844.1"
                     /translation="MTVLQNAALRKTQLVFGAVAGIQELKSKRLQKKCRALAEREKPF
                     RIMEFTNFLNCGQHHLFVLRKMEVNIPNTGMPDEKAFAGGVITGKRFIGLMVVDRVEE
                     KILVYQYARSNWTDRKFEENIRISYDSPVYALKHFTKVNIWFWQRELHILLNKTKGI"
     gene            15165..15560
                     /locus_tag="ACHKBL_003002"
     CDS             15165..15560
                     /locus_tag="ACHKBL_003002"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5819845.1"
                     /translation="MSKEENNIRFDPDNMTREEMVSELLLLRSSRDLTTDIYFEYVDA
                     GLPETTELTAADAGRLKEGLPAHHLHWLVGDGAPLTVRRISDFADVADDFSCLAAFID
                     PELAEEPEEGFVPEGFGGIFFCGIQDDDE"
     gene            complement(15614..15868)
                     /locus_tag="ACHKBL_003003"
     CDS             complement(15614..15868)
                     /locus_tag="ACHKBL_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003980153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFO5819846.1"
                     /translation="MTGWELRLWRKGMLWSREKAAREFGVTLRTWHAWENAEQVDVTV
                     LRTTQALSVMDLLPDIHRMTKSTIIRRLENEFRDGNTGDG"
BASE COUNT         4171 a         3704 c         4096 g         4100 t
ORIGIN      
        1 aaactcaggg tgaggctgtg gccagccgtg tgtagttgtt gttccgcgac attatcatgc
       61 gttccttcac gcgacggtcg atttcgcgct tcacggcctc caggcttccg cggaaacgcc
      121 cttccgcgat ttcacgtgtc agctgccgtt tcaccagggt gacaatgtcc tgacgggtcc
      181 tgcctgcatc gcggcgcgca cgggcacgtt tcatgccgtg ggacttaagc tctgcctgat
      241 aactacggaa acgctcgcgc acaaaacgcc aggctctcgc tatcagttca tccataccca
      301 gcgcatccag cccctgcttt ttgcgcagct tgttttccca ttccacgcgg ctgcggcgtg
      361 cggaagccac tgcctcttct gagatatcaa gtgagtcaaa cagtgccggc gtgaacgtga
      421 tgtcggtcgg gatgttacag ccaattgtcg ggtcgtattc cgtctggtag gttatcagcc
      481 ccagctcaga caggaacttc agggcacggg tggcacgggt gatggagagt ttcccggcct
      541 cagactccgt cgccagtccg cactcaatgg ccagcgtggt gatggagcac tgcacgcggt
      601 tggccagcgg gtcatagtgg aaacacagcc cctgcagcag cgcatcaatc gcccggcggc
      661 gcagcaccgg cggcatacgc cggcgcaggc cgcgcgaacg ggcatacgcc acatacacgg
      721 cgaaatcgaa acgggaagtg aaacccaccg ccttttccat cagtttttcg cagaacttca
      781 gcgttcctgc accttcacgg ggtgtgaaca ccggattcgg gttctttacc tggcggtaat
      841 acgtttgttg aagatcagtc acaccatcct gcacttacaa tgcgcagaag gagtgagcac
      901 agaaagaagt attgaacttt tccgggcata taactatact ccccgcatag ctgaattgtt
      961 ggctatacgg tttaagtggg ccccggtaat cttctgcgtc gccaaacata gaaaggttat
     1021 cggggttttt gcttttctgg ctcctgtaaa tccacatcag aaccagttcc ctgccacctt
     1081 acggcgtggc cagccatgaa ttttctgtcc gtaatctatc actctgccag aaaaggatca
     1141 aataaaggtg actactcacc atttttagct cgttccttct cctgctggat tagcagctcg
     1201 atcccttcac ccaaggtcaa atgcttatcc tggcaaaagc gctcaagaat atctttcaat
     1261 tcattacgga tgaaaacatt aagcgcttta tgtgtatccc gctttcgatt gagagacaac
     1321 ctctttcgct cattaacgct taacggtgca ccttttctat atgcccgttt aggtctggct
     1381 tcagaggaag ttgccgaacg tacagcgtca gacatacagc ctcctcatag atcaaaaaag
     1441 gatcattttg cagaggatac tacactttat tgaaatcagc gacttaatta taccctggcg
     1501 gatccatgtt ggcgcacaga tcgccagttc agctataaag atctgatgct attcagaaaa
     1561 gatcctgctc cgaacggaac aagtgtcgat atatcatgtg attacttaac taacaccctc
     1621 cacaaatccc ggatcaaaaa gggggcagct cacgaaacgg catttgaaca gaaacttacg
     1681 gtttcaactg ctgaacaaag aatgaaaccg taagttcttg aatgtgggat tacgttaacg
     1741 gtcggaactt gccttgctcg acccgctggt tttaaaaccc cgcccttaag ggcggggata
     1801 taaggcgcgg tgtataagtt acgctacatc aggaccatca tcacgctccg gcatacctga
     1861 tactctgaat cccttcatga cttcagattc acgagtcagc gatctacagt cccaaaccct
     1921 gcgatcgcca tttttttcaa tctctcttcc atccttcatc ctttcaatcc actgaacttt
     1981 ctctgttcct ttatcctcca gtcgcaaacg ctcaccttta ctataacctg ctacagcact
     2041 ctccagacca actccccata gtgttcgtgt tttcccgtta tccatcttca gtttcacaaa
     2101 atgggggttc gcgcccattt cctctaaaag tctcaccttg cttaggtaat taattgaatt
     2161 tatttataaa acgttgcaat tttagcatca ttttttattg ttctttttaa cctgaagtac
     2221 gtcgatcgag agatacctgt tttttctgtc acactgccca gagattctcc ctgtgatata
     2281 agctccaacg ctgtaggcgt tcgtggatgc ttcttcctgc caaattttac gcctgcggca
     2341 agtgccgcta ttcttccgtc gtttgtccgc tccagaatac gctcacgttc cgcttcagct
     2401 acagcagcaa ggatttggat aaccattttg cccattgtcc cttctgtact gagaccgttc
     2461 tccaggaacc ggatggcgat cccctttttg taacagctat caaccacacg aatcatgtca
     2521 gcagtgttac gccccagtcg gtccattttt gtacagatga ttacgtcgtc tttctctgca
     2581 cgggccaaca accgctgtag tccttcccgt tcatcagttg cgccagtcat catatccgta
     2641 aaaatacgat cttcccgtac cccggcattt tttagctccg agatttgatg tgttagcttc
     2701 tgatgactgg ttgatacccg tgcatacccc aataacgcca tttttagtct cacttatcgt
     2761 ttttgacact gcatggtatc ataatgtaca aaatacgatt tttgaaacct gcttttatgc
     2821 tgttttagac aggtatcaga tatcttaata tttgatacta gccatattta acaaatattt
     2881 agcattttgg taaatgtatg cagaagtaca agaaaagaat tagtatatta acaaaaagtg
     2941 aaataaatga attatatcaa gtcccctctt ttaatcctgt tgaaagggat gagttttttt
     3001 cgctggatat cagccttaaa agagaaattg ataaaataat aaatatagaa agcagggtgt
     3061 atctgatatt aataacaggg tatttcagat ataaacctgt gattcctgaa tttacattaa
     3121 aagacgtaaa aagggatgtg aaatatatta tccagacata ttataaaaac gaaagtacta
     3181 atctcaattt aaacatacct aaaagtacaa agtaccgcct tgttaaacga atgctttcta
     3241 ctctaggatt tgaacaactc accccatcta taaaaaatga gttaagtgaa cgtctgaatg
     3301 atgttgctac tatatgtatg gaaccaaaat atatttttga tgaattactt gcttttttag
     3361 gacagcggcg tatagctctt cctggataca gtactgtcca gaacctcgtt tcagatgctt
     3421 taaactttga aaggaaaagg acaattaata ttttgtcaaa gagaatttat cctgccactg
     3481 cacaaaagat tacatccatc atccatgatg atggaatatt aaactccatc aggggatata
     3541 attatagcac gagagacttc tcagcatcag aaatagaaaa tgaattaaga actcacaatg
     3601 tgataagtag tatttactat gaaataaaag atataattaa ttcattaggt atatcccagg
     3661 gaaacatgga gtactatgca actatcgtaa aacacagtac aacattcaga cttaagcgac
     3721 accctaaatg gcaaggaata ctttatttgt gctgctactt gtacttcaga tacagagaga
     3781 taaatgataa aataatcaca gccttcagat atttagtaaa aaaacataaa gacgcttcag
     3841 ttttgtttgc aaaagctcgg gcaacagagg aaatagatct aataaataaa aagcttaagt
     3901 ttgcgagtaa aatatttaac ttcttcgttg atagtaacat tgaagacagc atcccttttg
     3961 gatatgttcg tgaaaaggtt ttttcactta tatcgaaaga tgatatgcat cttgtgagtg
     4021 aattttttga caaaaataaa atcgatgtaa ttgaatatca atggcagtat attgacagcc
     4081 atcaccttaa gatcgccaat tcaatccgaa agctttttct ggctatagat atagtatatg
     4141 acaacgagaa gtctgttatt ggccagcaaa ttactgctgc caaggatgat ctcagaggaa
     4201 aaggaaagct ggatgctaag gatctgaggg taattcatcc atccgagaaa aattacattg
     4261 taaaagaagg agaaattaac actcaacgat tcgaatttca tatgtacagc agggtatcta
     4321 accatctgga aaacggtgtc ttttatgctg cggagagtga acaaaacaag agactggaag
     4381 atgatctcat agaacgaagt tactggcata agaataaatc gattttgata gagaaaacag
     4441 gccttgaacg acttatcaca ccaataacgc agactcttaa tgagctggag aataccttta
     4501 gcgataaact ggctcgtata accattggaa taaactcaga tgctaatgag tttgtgaaaa
     4561 aacaaccaca ctccaacaga ttacagtgga gtctggccag taaaaaatgg aagaccagca
     4621 tcgataatcc gatttacaat cagataaaaa atatcggaat agttgacgta atgaagtatg
     4681 taaacaatga gacgaatttt ttgtctgtgt ttaatgccat atcatccagg aaaaataatc
     4741 ttgaagctga ctataatgac cttcttgcct gtatatttgg taatggcgca aattatggtg
     4801 ttcacagaat ggcatctgca tcagaccgta gtattggaat actacgtgat gtgaatgaca
     4861 aattcattcg tccagaaaca acaggaatgg ccaacacagt aatatctgat gcaattgcaa
     4921 atctttctgt atataaatac tggacgataa atgaagattc tccatttgga agtgttgatg
     4981 ggcagaagca cacttgcaga ataaatacat ttaaagcccg ttattctgcc aagtatttca
     5041 gaaaggggaa aggggtttct gcgatgacac tggtatctaa tcatgtgcca ttatcaactg
     5101 tggtaatttc tccaaatgaa tatgaaggtc attttgcatt tgacttgtta tataacaaca
     5161 ccagtgaaat acaaccaaag tcattagcga ctgacaatca tggtataaac aacgttaatt
     5221 ttgcgattct tgacattttt ggctatcagt tttcaccacg ttatgcaaaa ttcaagaaaa
     5281 tatttaatga attatttgat gttatactgg agagtgataa actcattata aaacttaaaa
     5341 aacaatttaa ctttaagctc attgaacatg aatgggaaaa catccaacat attatttgtt
     5401 ctttaagcag aaaaacaacc attcaaagta cggttgttaa gaagttatct aatacaaagc
     5461 gtagcaacaa gacattagca gcacttcggg agtatgaccg agttataaaa tgcatatatg
     5521 tcctggatta tgctgacaat aaaaccttaa ggcaatttgt gcaacaagca ctgaacagag
     5581 gtgaagctta tcatcaacta agaagagcaa tagcatcagt aaatggaaat caatttcgcg
     5641 gtggtaatga ctaccaaata gatcaatgga acgactgtgc gaggttaata gctaattgtg
     5701 taatctatta taattcagca atattatcca ggctggtcga gaaatttgaa aaagaaaata
     5761 ataaaaaagc gatagatgtt ctggctaact tatccccagt agcatggaga cacattctcc
     5821 ttggagggaa ttattctttt gaagatcaag ttgcgatcac aaatttggac agtcttttgg
     5881 agggagtaga cccactaagt gatatcggag atattgagtc cgaatatgac ggttaacaac
     5941 atgataaata atggttttta tgctaagagt gagacttttg gaggaaatgg gcgcagaacc
     6001 ccgtaaagga tgttcacctg ataatgccgc atgcgaaggc tttttcggca gactgaaaac
     6061 ggaaatgtat tatgggcgta aatggtcggg catcacgcca gaaaagttca tgcagcaagt
     6121 ggatgcttac atcagatggt ataacgagcg gcgtataaaa ttctcgctgg gtgccgtcag
     6181 ccccaaaatg taccgctaac aatgcgggct ggaatgataa agcagtccag gaaatcgtcc
     6241 gcatccccaa aacggccaat aatcttggcg ttgacaccac cgccagatca gtggcagaag
     6301 tgccgtctgc gggcagacct gcggccggga ccagcggcag tacgacgact tctgccgcac
     6361 gctgttctgc ccggaggcgg tcctgttctg ccagcagcag aggctgtgcc acggcccggc
     6421 cggcgtccgt cagctccact gcctgctgca ggttcagttt ttttctgaat tttaactgta
     6481 tttgtccttt attgttcttg tttgttccct ggtgtgctat ttgagtcggt ttttgtcttc
     6541 atttttcttt atttttctgt ttgtggacga tatcaggtct ttactgatgc gtgtttttag
     6601 gtgataatct ttttcggcgc catatttttc taaaaaaatc cattttattc tgtgaggttc
     6661 tggatgcgta tctttgtaga tgacggttct accaacatca aactggcctg ggttgaagac
     6721 ggtgcagtga aaacgttaat tagtccgaat agttttaaac cggaatggtc tatgacgctg
     6781 cagggactga ctgatgccac tatacccgct tccgcaaact atgagattga tggagagaaa
     6841 tacagctttg atcaattgag tccggatgca gtgcgcacca cagaaacgcg ttatcagtac
     6901 agcgacgtta atgtggttgc tattcatcat gcgttgatgc agtcaggtat tgctccgcaa
     6961 cctgttgata ttgttgtgac tttaccttta ggtgagtatc tggatgagaa cgatcagcca
     7021 aatctggcta atatcgaacg taagaaaaat aatgtcagac gagctgtaac agttcagggg
     7081 cgtgaaaact ttaccattcg taaagtcagt gtcttgcctg agtccgtgcc tgccggtttt
     7141 gacgtgctga aagatctgaa tgatctggag tcgctgttga tcgttgatat cggtggaacg
     7201 acccttgata ttgcacatgt ccgcagcaaa atgtccggtt tcacaaaaac ttattgtgac
     7261 cccaagacag gcgtttccat cattactgaa gcagttaagc atgcgatgga tgtgaatgtg
     7321 agtacacgaa ccagttctta ttttgcagat cgtctaatcc agggccggga tgacaatgaa
     7381 ttcctgactc gttatatccc gaatgcagaa cagcgtgagc agatcctgac agtattatgt
     7441 gaccgggaaa aaaaactgca gcatcgtgtg gcggattcag taggacgctt tgctggtttt
     7501 actcatgtta tggttgttgg cggtggtgcc agccttgtgg ctggggcagt aaaaaaagcg
     7561 acaggagtgg gtgataaccg tttctttgtc agtgacaatc ctcagttcga tctggtgttg
     7621 ggaatgatgg caatgaaggg atgagtatgg ctgataatcg taagtgctct ttttatattt
     7681 atccggaacg aaatgcagct gatcgggtgg ctgacaggtt tcttgaaaaa ttgcctcaga
     7741 aagaacgtgg tcgggcaatg cgggccatga tgttgtgtgg tgctgcattg atgaaacagg
     7801 atgaacgatt gccgttcctg atagctgaat tcctgacaga cagcacgtct atgcaggata
     7861 ttcaacgaat tatcagtagt acgctaccac aacaggaaaa tggtgaagtt gttcgtttgc
     7921 tggaggcatt tctgcagtct gccggtaata atgcaaaaac gatcctgcct gcggttgatt
     7981 ctgcttcgca agaaatatct gctccagtcg atcagaatct cctggaaaca agaaacaaca
     8041 ttaagaactt gtttccggat gatgaataag aactgaactc tgttaaagga cgctgttgcg
     8101 tccttttttc ttttccgaca aaggatgtcg ccgggctcct ctttttactg cgccggctga
     8161 cgcggcgcgg cagaaacgct gcctgtggtc agtgtcccgc gtccggcggg cacgggacgg
     8221 gcgcttttac cgctcccggc tggcgtcggt gacagtgtac gccagccagt cgcccttttc
     8281 tgatgaacgc ccttcaagcc gctttcgcgg cataaccttg ccgtcaaaaa gacgacggct
     8341 gcggtattcc acggtcggcc tgacccgtta ccggacgcgg tgaacagccc acaggcagcg
     8401 gggaacgggc gctacagggg cgccctcccg gtgctctctg ctgaagcaga agtgagagga
     8461 aaattcccat gccaaactgg tgctcgaatc gtatgtattt ttctggtgaa ccggcacaga
     8521 tcgctgagat taaacgactg gccagcggcg cagtcacacc gttttatcgc cgcgccacaa
     8581 atgaaggtat tcagctgttt ctggccggaa gtgccggact tctgcagacc actgaagatg
     8641 tgcagtttga accgtgcccc ggactgacgg ctgccggacg tggtgttgta tcgccggaga
     8701 atatcgcgtt cacccgctgg ctgacacacc tgcagaacgg tctactgctg gatgagcaaa
     8761 actgcctgat gctgcatgaa ctctggctgc agagcggtac tggacagcgt cgctgggaag
     8821 gattaccgga tgatgtcagg gataccatca ccgcactttt caccgcaaaa agaggtgact
     8881 ggtgtggctt ctggagtaac gaggatgtat cggtgtggtg gaaccgtctg tgtgacaacg
     8941 tggttccgga aaaaaccatg ccgtttgacc tgctgacggt tctgcccacc cgcctggatg
     9001 ttgaagtgaa tggctttaac ggtggtgttc tgaacggtgt tccttctgca tatcactggt
     9061 atacggaaca gtatggcgtg aagtggcctg tggggtatga ggtgaatatc agtagtcagg
     9121 gagacaactt cattcaggtt gatttcgaca cgccgtggtg tcagccggaa agcgacgtta
     9181 ttgcagaatt aagccgccgt ttcagctgca cgctggagca ctggtatgcc gaacagggct
     9241 gtgatttctg tggctggcag ttgtatgagc gcggagagct cgttgatgtg ctgtgggggg
     9301 aactggaatg gtcttccccg acagatgacg atgagctgcc ggaagtcacc ggacctgcgt
     9361 ggatagtcga caatgtggcg cattatggcg gatgaagtat gacggagacg ggcgggaaac
     9421 cgcccgtttc ttttccgaca aaggatgtcg ccgcaggctc cgctttaccc ggccatgcgg
     9481 ggcatggcct tgtgggtttt cagttatgtg gcctcagcgt cgtgtgcggg ctgtgccgtg
     9541 cctccatcgt agccgggctt ctgcgggcgg gttgcaaggt aaggtgaacc gctgcggtca
     9601 cccggtccct ccttcccgtc tgccgtgatt ttccggtcgt tcacccgctc aggtttcgcg
     9661 gtctcaccct ccaactcccg tcagccgtgt gcgtcaggct gcggcttccc ttgcgccctt
     9721 gcaaccctgc aggcccggca aagatggagg cacggcaccg cccggtgtcg cggaatatgt
     9781 gaacaacgga ggaaatccca tgcagtatgc gaaatctgtc actctgaacg ttgaagagtg
     9841 cgaccgcctt tcttttctgc cttacctgtt tggcaacgat tttctgtatg ccgaggcgta
     9901 tgtgtacgcg ctggcgcaaa aaatgatgcc ggaatatcag ggaggcttct ggcatttcat
     9961 ccgcctgccg gacggtggcg gttacatgat gccggatggc gaccgtttcc acatggtgaa
    10021 cggtgcaaac tggtttgacc gtaccgtgag tgctgatgcc gcgggcgtca ttctgacttc
    10081 ccttgtgatt aaccgccagt tgtggctgta ccacgacagc ggggatgcag gactgaccca
    10141 gctttaccgg atgcgcgatg cgcagttgtg gcgtcacatc gaatttcacc ctgaatgcaa
    10201 cgcgatttac gcagcactgg actgattaac ggacggggcg gcaatgccgc ccctgtaaag
    10261 gagacgagaa catgtactgt actgttaaag aaatcatccg cgatgtactg gatacagacg
    10321 tgccggacag tgaatgcgtt tttgccgtgg tgctgacccg tggggatgtg cgtcacatag
    10381 ctcaggactg gagtctgaca gacgatgagc tggaaaccat catgcagcgg ctggacgatg
    10441 cctttgagca tggtgcggat gtcagcgttg ttcacgacgt tgttcgggaa ctgatggaag
    10501 aaaagcgcgc cagccgtcag gtgacagtcc cggcggtgat gctggaaaaa gtgatggcgc
    10561 tggcaggcag tgaaatgaag cgcctgtatg ccgtcgggag tgagaacggg ggcgacggtg
    10621 acgcattcgt cagggaagaa cgcgaagcga tggacgttgt gttacaggcg ctggacgggg
    10681 agaacatgtc atgaatatca gcacagaaac ccgcgaaatt ctgcgcaatt acaaagccgt
    10741 gattaatgca cgacgtcgtg agatggggca gaaaccgctc accaccgcac agattgttga
    10801 tgaaatctgc gattttgtgg cgaatcagca ggcggttttc ctcggtggtc actacatcct
    10861 tcagggcagc aggcacaggt gatacagggc aggcggtgga ggccgcctgt cagcccgcgc
    10921 gaaaacccct ggcaggctga cgcctgccgg aaatgcgggg tgcagcgcct gcggcgctgc
    10981 cggcccggct ttccggtcag aagatagtgt atggctgtat gccaggaaca ggtgcgatat
    11041 tccgtgacac tgtcactgtt tctggctgtc ttttattgcg cccacttcgg ctcccggctg
    11101 cctggcggca tccggtcgtc aggcccgact acgacaggca gctgcttgcg cacccgccag
    11161 tctccgccga acccgctggc gcggtttcgg gctgtcgcta cggtccgatg cgtgaaaaac
    11221 cgggcttgca gcgaccgttc cggtcgcgcc gcctctgtcg cgccgtgacc tgcgctccct
    11281 gcacacctga cggtgtgtga ggaaactgcg cctgatggcg cagcagtctt ccctcctgcc
    11341 gccacctctg cggcctgttt cccgctgtgg actttccggg gggcagacgt aaccccctcc
    11401 cgttccatct gtccagggtg agatgcaccg gccacaaaat ttttagccat gaacccggca
    11461 aaaaattttg cgtccgcccc cggacagctg tcccgtgagg cggtaaacgg gcccccatcg
    11521 gaaaggtcca cagcgggaag aatccccgga gaagtggacg gcaggaggag cttcgctcct
    11581 taagaagcgg cctgaaacat ccggaccgct ctttaacaat ccgaccattt tttcagtgaa
    11641 tcagaaaaca ctgaaggcgg tgatggcgat acagacgccg ttcagcgcaa ggaacgctgc
    11701 agaggaaggg agagccggaa gtgtgtaaag gtgggctgaa acgccctttt atcagaacgt
    11761 tcaggaggtg aatcatgaca caggcagtta tttcgaatat ttgtgcagcg ctggggaatg
    11821 gctggatgat taatccgtcc ccgaaacagg atttaataaa aggttatttt cagttagtca
    11881 gtgcggagaa aaaatatgaa catttttcaa tgtactgctt tgtaatgaac ggcatgcttc
    11941 atatctacgg gtgtgtatat gatgaactga acggggatat ggcctggatc cccctgaaca
    12001 agtcgatgcc tgacattgtg cgaattataa aaagaaaagt gatatctcag aaaaatcgtc
    12061 tgctttccat tgtgagttac aggaagtaag gccgcagcgc cacaggcggc aggctgcggt
    12121 gtcagcctgc ccggtcagtt cagtcagcac gtgttaacag gagataaaaa tgaattattc
    12181 agagcaggtt gcgcagatta ttctggagca actcgggggt aaacgtttta ttgccatgac
    12241 cggagccagt aatctggcgt attttgatga tgaaggtgag agcggattat gttttcgttt
    12301 gccgtctcat tttgcaataa ataatattaa tatagtcaga attaaattaa cgtttgccga
    12361 tgattacacg gtgacgtttt acagcaaaca gggttcccgg atagcgcaga tatcggagca
    12421 cgaggggatt tattgcgatc agctgcaatg gctttttacc cgggagacag ggctgtatac
    12481 acgactgtaa atccctgccc ttctttctgc ccgtggcagg tgcgtaccgc acgggcgtat
    12541 aatcccggtg ataataactc atggagggaa agtgagtaag aagaccccca cgacgcccac
    12601 gccgcatgat ggcgcgttcc gggcgttcct gtccagtcct gaagtggccc gtgattttgt
    12661 gtcacttcat ctgccggcgg aatatcgcgg actgtgcgac ttgtcgacgc tgaaaattga
    12721 gccgtgtagc tttgtggatg aggagctcaa aaaatatgaa tgcgatgccc tgttcagcat
    12781 gaagacgccg gatggtgagg atggttatgt ttattgcctc gcggaacacc agagcagtga
    12841 caatccgttc atggcctttc gtatgctcag atattcagtg gcggcaatgc agcggcattt
    12901 tgagcagcat aaaaggctgc cgctggttat tccgatactg ttttatcatg gagaacggag
    12961 tccgtatccg cacagtatgg gctggatgga ctgtttcgac aaaccggaaa tagttgctaa
    13021 aattttcacc agcccgtttc acctggttga tctgacggtg acggatgatg atgagttcat
    13081 gcaccatcgc cgtatggggg cactgagtct catgatgaag catatccgca gtcgtgatct
    13141 gatgatgcag cttgacagac tggcgacact gatacgggag acgctggatg aaaggctgac
    13201 gctgatactg gtgaattatc tgctgaatgg cagcgaacat gtaacggttg aatttttgca
    13261 gacgctggcg cagcgactgc cgcagcacga ggacagcatt atgacactgg cagaacgtct
    13321 taagcaggaa ggaatacaga aaggtcgaat ggaaggccgc atggaaggtc gaatggaagg
    13381 ccgcatggaa ggccgtctgg aggagaagca ggatattgcg cgtcagcttc agaaaatggg
    13441 gatgacgccg gagcagatta agctggccac cggactttcc gatgacgacc tgaagaaaat
    13501 cacgcactga tgagaaacac agagcccggc aacatagccg ggctttttcg ttctgttacc
    13561 ggtagcgcag cccgtgtgac cgggcatcga acccggtcac acgggataag gagccacatt
    13621 ttctggcaga tggtctgaac agccacggag ccgtggtgtt tctgcctgta tccatccact
    13681 aaaaaagtga agttttttca ctttttcttt tttgttttca tgccgtttcc gacactacct
    13741 cattaatcaa tgtgtggcac agaatccgcc ggtattgccg ggcagatatt gtgcggtgtt
    13801 cgtcactact ggtgatgcgt cctccccggg ggcgtgcagc gaccgtaccg gtcgcgcgcc
    13861 ccggtcgcgc cgttggcggc gctccacgca tcccggccag gggccgccgg gatgtaatgc
    13921 caccgcccct gtcggcgcgg tggtcctcct tcagcctgtc atcccgtgcc ctgcatcccc
    13981 gcagaggact ttccgggggg cagacgtaac catcacccgc tccatcagtc caggggtgaa
    14041 atgcacatcc cgcaaaactt tttgcccaaa agcaggcaaa aactttagcg tgctgccccc
    14101 ggacagctgt cccgtgagct ggtaaacggg cccccatcgg aaaggtccac agcggggagt
    14161 cacctgtacg ggaccggacg gcagaaggag cttcgctcct cagaagatgc ctgaacatac
    14221 cgtaccgttc tttaacaatc tgaccttttt tcagcgtaaa gagaaaaagc gtaaggaggt
    14281 gagcacgatg taaatcacag acggcgcgag ggaatgcagg aaaggagaaa aggaggttcg
    14341 caggagtgca aggggtgcag tcacacccct ttgcacgaat atcaacacat cacttggagg
    14401 catgattatg tcaatgcgac aaatcgtaac ccttgctgat gcggttgtca aagggcaacc
    14461 cacacgcatt ccgccaatga aaggcgacga atggcgcgag ttctgttggt ggctggacca
    14521 cctcagaggc tatgccatgt gacacccggg gggcggtaac gccccctttt taagacggtt
    14581 tcatacagtc cctgacggag gcaattatga cagtactgca gaatgcagcg ttacggaaga
    14641 cacagcttgt ttttggtgct gtggccggaa tacaggaatt aaaaagtaag cggcttcaga
    14701 aaaagtgtcg ggcgctggcg gagcgtgaaa aaccgtttcg tatcatggag ttcacgaatt
    14761 ttttaaactg tggtcagcat catttattcg tgctgcggaa aatggaggta aatatcccga
    14821 acacagggat gccggatgag aaagcctttg caggcggcgt gataaccgga aagcgcttta
    14881 ttggtctgat ggttgttgac cgcgtggagg aaaaaatcct ggtttatcag tatgcacgca
    14941 gtaactggac agaccggaaa tttgaagaaa atatccgaat cagctatgac agcccggttt
    15001 atgcactgaa gcatttcacg aaggtgaaca tctggttctg gcaaagggaa ttacatatcc
    15061 tgttgaacaa aacgaaaggc atataaatcc gtgcggggcc ggacagcccc gccagttctg
    15121 tcgcagagct gcggcatggt gatactgaat gagatacaga gacaatgagt aaagaggaaa
    15181 acaatatccg gtttgacccg gataacatga cgcgcgaaga gatggtcagt gagcttttac
    15241 tgctccgttc gtccagggac ctgacgacgg atatttattt tgaatatgtg gatgcggggt
    15301 tacccgaaac aaccgaactc accgccgcag atgccggacg cctgaaagaa gggttacccg
    15361 ctcatcatct tcactggtta gtcggggatg gcgcacccct gacggtaagg cgaataagtg
    15421 actttgccga tgttgcggat gatttcagtt gcctggctgc tttcattgat cctgaactgg
    15481 cggaggagcc ggaagaaggc tttgtgccgg aagggttcgg ggggattttt ttctgcggta
    15541 ttcaggatga tgatgaataa ggcattgccg catttccggt catgatgcgc attccggcgg
    15601 aacgggcggt cagtcatccg tcgccggtgt tcccgtcgcg gaattcattc tccagacgcc
    15661 ggatgatggt gctttttgtc atccggtgta tatccggcag taaatccatg acagaaagtg
    15721 cctgggtggt tctcaggacg gtgacatcca cctgttccgc attttcccag gcgtgccatg
    15781 ttctcagcgt cacgccaaat tctctggccg ctttttcgcg tgaccagagc attccttttc
    15841 tccagagtct cagttcccag ccggtcataa atcacctttg aaaaagtgaa aaaacttcac
    15901 tttattttat catcatagtc accaagtcag cgcgataaaa aagagtgaaa aagtgaagaa
    15961 atttcacttt tttttgattg tatatgtcgt gttgttttct ctgttttcac ctgtgcggga
    16021 gtggtgcacc agtgaccacg acatgccatt gttgtgccgt cgtttttctt c
//
LOCUS       DAZFLD010000113        15744 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.114, whole genome shotgun sequence.
ACCESSION   DAZFLD010000113 DAZFLD010000000
VERSION     DAZFLD010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15744
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.114"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..121
                     /gene="yecU"
                     /locus_tag="ACHKBL_003004"
     CDS             <1..121
                     /gene="yecU"
                     /locus_tag="ACHKBL_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protein YecU"
                     /protein_id="HFO5819847.1"
                     /translation="TLKKQPLLFLAKLMVYSAALTFFTANFHCNMTRKINEYA"
     gene            108..332
                     /gene="yecF"
                     /locus_tag="ACHKBL_003005"
     CDS             108..332
                     /gene="yecF"
                     /locus_tag="ACHKBL_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2594 family protein YecF"
                     /protein_id="HFO5819848.1"
                     /translation="MSTPDFSTAENNQELANEVSCLKAMLTLMLQAMGQADAGRVMLK
                     MEKQLALIEDETQAAVFSKTVKQIKQAYRQ"
     gene            complement(399..1121)
                     /gene="sdiA"
                     /locus_tag="ACHKBL_003006"
     CDS             complement(399..1121)
                     /gene="sdiA"
                     /locus_tag="ACHKBL_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SdiA"
                     /protein_id="HFO5819849.1"
                     /translation="MQDTDFFSWRRTMLLRFQRMEAAEEVYHEIELQAQQLEYDYYSL
                     CVRHPVPFTRPKVAFYTNYPEAWVSYYQAKNFLAIDPVLNPENFSQGHLMWNDDLFSE
                     AQPLWEAARAHGLRRGVTQYLMLPNRALGFLSFSRCTAREIPILSDELQLKMQLLVRE
                     SLMALMRLNDEIVMTPEMNFSKREKEILKWTAEGKTSAEIAMILSISENTVNFHQKNM
                     QKKINAPNKTQVACYAAATGLI"
     gene            complement(1351..2103)
                     /gene="tcyN"
                     /locus_tag="ACHKBL_003007"
     CDS             complement(1351..2103)
                     /gene="tcyN"
                     /locus_tag="ACHKBL_003007"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine ABC transporter ATP-binding protein
                     TcyN"
                     /protein_id="HFO5819850.1"
                     /translation="MSAIEVKNLVKKFHGQTVLHGIDLEVKPGEVVAIIGPSGSGKTT
                     LLRSINLLEQPEAGTITVGDITIDTARSLSQQKSLIRQLRQHVGFVFQNFNLFPHRTV
                     LENIIEGPVIVKGEPKEEATARARELLAKVGLAGKETSYPRRLSGGQQQRVAIARALA
                     MRPEVILFDEPTSALDPELVGEVLNTIRQLAQEKRTMVIVTHEMSFARDVADRAIFMD
                     QGRIVEQGAAKALFADPEQPRTRQFLEKFLLQ"
     gene            complement(2100..2768)
                     /gene="tcyL"
                     /locus_tag="ACHKBL_003008"
     CDS             complement(2100..2768)
                     /gene="tcyL"
                     /locus_tag="ACHKBL_003008"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter permease"
                     /protein_id="HFO5819851.1"
                     /translation="MQESIQLVIDSLPFLLKGAGYTLQLSIGGMFFGLLLGFILALMR
                     LSPIWPVRWLARFYISIFRGTPLIAQLFMIYYGLPQFGIELDPIPSAMIGLSLNTAAY
                     AAETLRAAISSIDKGQWEAAASIGMTPWQTMRRAILPQAARVALPPLSNSFISLVKDT
                     SLAATIQVPELFRQAQLITSRTLEVFTMYLAASLIYWIMATVLSTLQNHFENQLNRQE
                     REPK"
     gene            complement(2783..3769)
                     /gene="dcyD"
                     /locus_tag="ACHKBL_003009"
     CDS             complement(2783..3769)
                     /gene="dcyD"
                     /locus_tag="ACHKBL_003009"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019148 - D-cysteine desulfhydrase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HFO5819852.1"
                     /translation="MPLHNLTRFPRLEFIGAPTPLEYLPRFSDYLGREIFIKRDDVTP
                     MAMGGNKLRKLEFLAADALREGADTLITAGAIQSNHVRQTAAVAAKIGLHCVALLENP
                     IGTTAENYLTNGNRLLLDLFNTQIEMCDALTDPNAQLEELATRVEAQGFRPYVIPVGG
                     SNALGALGYVESALEIAQQCEGAVNISSVVVASGSAGTHAGLAVGLEHLMPESELIGV
                     TVSRSVADQLPKVVNLQQAIAKELELTASAEILLWDDYFAPGYGVPNDEGMEAVKLLA
                     RLEGILLDPVYTGKAMAGLIDGISQKRFKDEGPILFIHTGGAPALFAYHPHV"
     gene            complement(3874..4674)
                     /gene="tcyJ"
                     /locus_tag="ACHKBL_003010"
     CDS             complement(3874..4674)
                     /gene="tcyJ"
                     /locus_tag="ACHKBL_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015276 - ligand-gated monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO5819853.1"
                     /translation="MKLAHLGRQALMGVMAVALVAGMSVKSFADEGLLNKVKERGTLL
                     VGLEGTYPPFSFQGDDGKLTGFEVEFAQQLAKHLGVEASLKPTKWDGMLASLDSKRID
                     VVINQVTISDERKKKYDFSTPYTISGIQALVKKGNEGTIKTADDLKGKKVGVGLGTNY
                     EEWLRQNVQGVDVRTYDDDPTKYQDLRVGRIDAILVDRLAALDLVKKTNDTLAVTGEA
                     FSRQESGVALRKGNEDLLKAVNDAIAEMQKDGTLQALSEKWFGADVTK"
     gene            complement(4762..5313)
                     /gene="fliZ"
                     /locus_tag="ACHKBL_003011"
     CDS             complement(4762..5313)
                     /gene="fliZ"
                     /locus_tag="ACHKBL_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliZ"
                     /protein_id="HFO5819854.1"
                     /translation="MMVQHLKRRPLSRYLKDFKHSQTHCAHCRKLLDRITLVRDGKIV
                     NKIEISRLDMLLDENGWQVEQKSWAALCRFCGDLHCKTQSDFFDIIGFKQFLFEQTEM
                     SPGTVREYVVRLRRLGNHLHEQNISLDQLQDGFLDEILAPWLPTTSTNNYRIALRKYQ
                     HYQRQTCTGLVQKSSSLPASDIY"
     gene            complement(5359..6078)
                     /gene="fliA"
                     /locus_tag="ACHKBL_003012"
     CDS             complement(5359..6078)
                     /gene="fliA"
                     /locus_tag="ACHKBL_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
                     /protein_id="HFO5819855.1"
                     /translation="MNSLYTAEGVMDKHSLWQRYVPLVRHEALRLQVRLPASVELDDL
                     LQAGGIGLLNAVERYDALQGTAFTTYAVQRIRGAMLDELRSRDWVPRSVRRNAREVAQ
                     AIGQLEQELGRNATETEVAERLGIDIADYRQMLLDTNNSQLFSYDEWREEHGDSIELV
                     TDDHQRENPLQQLLDSNLRQRVMEAIETLPEREKLVLTLYYQEELNLKEIGAVLEVGE
                     SRVSQLHSQAIKRLRTKLGKL"
     gene            complement(6397..8136)
                     /locus_tag="ACHKBL_003013"
     CDS             complement(6397..8136)
                     /locus_tag="ACHKBL_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001602758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliC/FljB family flagellin"
                     /protein_id="HFO5819856.1"
                     /translation="MAQVINTNSLSLITQNNINKNQSALSSSIERLSSGLRINSAKDD
                     AAGQAIANRFTSNIKGLTQAARNANDGISVAQTTEGALSEINNNLQRIRELTVQASTG
                     TNSDSDLDSIQDEIKSRLDEIDRVSGQTQFNGVNVLAKDGSMKIQVGANDGQTITIDL
                     KKIDSDTLGLSGFNVNGGGAVANTAATKSDLAAAQLLAPGTADANGTVTYTVSAGLKT
                     STAADVIASLANNAKVNATIANGFGSPTATDYTYNSATGDFTYSATIAAGTNSGDSNS
                     AQLQSFLTPKAGDTANLNVKIGSTSIDVVLASDGKITAKDGSELFIDVDGNLTQNNAG
                     TVKAATLDALTKNWHTTGTPGAVSTVITTEDETTFTLDGGTDATTSGAITVANARMSA
                     ESLQSATKSTGFTVDVGATGTSAGDIKVDSKGIVQQYTGTVFEDAYTKADGSLTTDNT
                     TNLFLQKDGTVTNGSGKAVYVSADGNFTTDAETKAATTADPLKALDEAISSIDKFRSS
                     LGAVQNRLDSAVTNLNNTTTNLSEAQSRIQDADYATEVSNMSKAQIIQQAGNSVLAKA
                     NQVPQQVLSLLQG"
     gene            8402..9808
                     /gene="fliD"
                     /locus_tag="ACHKBL_003014"
     CDS             8402..9808
                     /gene="fliD"
                     /locus_tag="ACHKBL_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0009421 - bacterial-type flagellum
                     filament cap [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HFO5819857.1"
                     /translation="MASISSLGVGSGLDLSSILDSLTAAQKATLTPISNQQSSFTAKL
                     SAYGTLKSALTTFQTANTALSKADLFSATSTTSSTTAFSATTAGNAIAGKYTISVTHL
                     AQAQTLTTRTTRDDTKTAIATSDSKLIIQQGGDKDPITIDISAANSSLSGIRDAINNA
                     KAGVSASIINVGNGEYRLSVTSNDTGLDNAMTLSVSGDDALQSFMGYDASASSNGMEV
                     SVAAQNAQLTVNNVAIENSSNTISDALENITLNLNDVTTGNQTLTITQDTSKAQTAIK
                     DWVNAYNSLIDTFSSLTKYTAVDAGADSQSSSNGALLGDSTLRTIQTQLKSMLSNTVS
                     SSNYKTLAQIGITTDPSDGKLELDADKLTAALKKDASGVGALIVGDGKKTGITTTIGS
                     NLTSWLSTTGIIKAATDGVSKTLNKLTKDYNAASDRIDAQVARYKEQFTQLDVLMTSL
                     NSTSSYLTQQFENNSNSK"
     gene            9833..10243
                     /gene="fliS"
                     /locus_tag="ACHKBL_003015"
     CDS             9833..10243
                     /gene="fliS"
                     /locus_tag="ACHKBL_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000270662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HFO5819858.1"
                     /translation="MYAAKGTQAYAQIGVESAVMSASQQQLVTMLFDGVLSALVRARL
                     FMQDNNQQGKGVSLSKAINIIENGLRVSLDEESKDELTQNLIALYSYMVRRLLQANLR
                     NDVSAVEEVEALMRNIADAWKESLLSPSLIQDPD"
     gene            10243..10608
                     /gene="fliT"
                     /locus_tag="ACHKBL_003016"
     CDS             10243..10608
                     /gene="fliT"
                     /locus_tag="ACHKBL_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:1902209 - negative regulation of
                     bacterial-type flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliT"
                     /protein_id="HFO5819859.1"
                     /translation="MNNAPHLYFAWQQLVEKSQLMLRLATEEQWDELIASEMAYVNAV
                     QEIAHLTEEVAPSTTMQEQLRPMLRLILDNESKVKQLLQIRMDELAKLVGQSSVQKSV
                     LSAYGDQGGFVLAPQDNLF"
     gene            10686..12173
                     /gene="amyA"
                     /locus_tag="ACHKBL_003017"
     CDS             10686..12173
                     /gene="amyA"
                     /locus_tag="ACHKBL_003017"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HFO5819860.1"
                     /translation="MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPA
                     YKGASGGYSVGYDSYDLFDLGEFDQKGSIPTKYGDKAQLLAAIDALKRNDIAVLLDVV
                     VNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGQYSQFIWDFKCF
                     SGIDHIENPDEDGIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKY
                     WARWVMEQTQCDGFRLDAVKHIPAWFYKEWIEHVQEVAPKPLFIVAEYWSHEVDKLQT
                     YIDQVEGKTMLFDAPLQMKFHEASRMGRDYDMTQIFTGTLVEADPFHAVTLVANHDTQ
                     PLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPII
                     EQLDELILARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTIHLG
                     ENYGNKTWRDFLGNRQESVVTDENGEATFFCNGGSVSVWVIEEVI"
     gene            complement(12207..12620)
                     /gene="yedD"
                     /locus_tag="ACHKBL_003018"
     CDS             complement(12207..12620)
                     /gene="yedD"
                     /locus_tag="ACHKBL_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310694.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YedD"
                     /protein_id="HFO5819861.1"
                     /translation="MKKLAIAGALMLLAGCAEVENYNNVVKTPAPDWLAGYWQTKGPQ
                     SALVSPEAIGSLIVTKEGDTLDCRQWQRVIAVPGKLTLMSDDLTNVTVKRELYEVERD
                     GNTIEYDGMTMERVDRPTAECAAALDKAPLPTPLP"
     gene            12807..14012
                     /gene="yedE"
                     /locus_tag="ACHKBL_003019"
     CDS             12807..14012
                     /gene="yedE"
                     /locus_tag="ACHKBL_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
                     /protein_id="HFO5819862.1"
                     /translation="MSWQQFKHAWLIKFWAPIPAVIAAGILSTYYFGITGTFWAVTGE
                     FTRWGGQLLQLFGVHAEEWGYFKIIHLEGSPLTRIDGMMILGMFGGCFAAALWANNVK
                     LRMPRSRIRIMQAIIGGIIAGFGARLAMGCNLAAFFTGIPQFSLHAWFFAIATAIGSW
                     FGARFTLLPIFRIPVKMQKVSAASPLTQKPDQARRRFRLGMLVFFGMLGWALLTAMNQ
                     PKLGLAMLFGVGFGLLIERAQICFTSAFRDMWITGRTHMAKAIIIGMAVSAIGIFSYV
                     QLGVEPKIMWAGPNAVIGGLLFGFGIVLAGGCETGWMYRAVEGQVHYWWVGLGNVIGS
                     TILAYYWDDFAPALATDWDKINLLKTFGPMGGLLVTYLLLFAALMLIIGWEKRFFRRA
                     APQIAKEIA"
     gene            14009..14242
                     /gene="yedF"
                     /locus_tag="ACHKBL_003020"
     CDS             14009..14242
                     /gene="yedF"
                     /locus_tag="ACHKBL_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HFO5819863.1"
                     /translation="MKNIVPDYRLDMVGEPCPYPAVATLEAMPQLKKGEILEVVSDCP
                     QSINNIPLDARNHGYTVLDIQQDGPTIRYLIQK"
     gene            14351..15019
                     /gene="yedK"
                     /locus_tag="ACHKBL_003021"
     CDS             14351..15019
                     /gene="yedK"
                     /locus_tag="ACHKBL_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HFO5819864.1"
                     /translation="MCGRFAQSQTREDYLAFLAEDIERDIPYDPEPIGRYNVAPGTKV
                     LLLSERDEHLHLDPVFWGYAPGWWDKPPLINARVETAATSRMFKPLWQHGRAICFADG
                     WFEWKKEGDKKQPYFIYRADGQPVFMAAIGSTPFERGDEAEGFLIVTAAADQGLVDIH
                     DRRPLVLSPEAVREWMRQEVGGKEASEIAASGCVPANQFTWHPVSCAVGNVKNQGAAL
                     IQPV"
     gene            15130..15609
                     /gene="yedL"
                     /locus_tag="ACHKBL_003022"
     CDS             15130..15609
                     /gene="yedL"
                     /locus_tag="ACHKBL_003022"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO5819865.1"
                     /translation="MFTIKTDDLTHPAVQALVAYHISGMLQQSPPESSHALDVQKLRN
                     PKVTFWSVWEGEQLAGIGALKLLDDKHGELKSMRTAPNYLRRGVASLILRHILQVAHD
                     RCLHRLSLETGTQAGFTACHQLYLKHGFVDCEPFADYQLDPHSRFLSLTLCENNELP"
BASE COUNT         3894 a         4022 c         3860 g         3968 t
ORIGIN      
        1 cacgctcaaa aaacaaccac tgctattttt agcaaagctt atggtatact ccgccgcctt
       61 aacatttttc accgcaaatt ttcattgcaa catgacgagg aaaataaatg agtacgcctg
      121 atttttctac tgccgagaat aatcaagaac tggcaaatga agtctcctgc ctgaaagcga
      181 tgctgacgct gatgctgcag gcgatgggac aagctgacgc gggccgcgtg atgttaaaga
      241 tggaaaaaca gcttgcgctg atcgaagacg aaacccaggc tgcagtattt tccaaaacgg
      301 ttaagcaaat taaacaagcc taccgtcagt aatataaaac cggctgagag cgtgcctttc
      361 agccggtttt tgcatctggc acgcagaaag aaaagcgatc aaattaagcc agtagcggcc
      421 gcgtaacagg ccacctgggt cttatttggt gcattgattt ttttctgcat gtttttctga
      481 tggaaattga ccgtattctc agagattgac aaaatcatcg ctatctctgc tgatgtcttc
      541 ccttccgccg tccatttcag aatttctttt tcgcgcttgc tgaaattcat ctctggcgtc
      601 atcactattt catcatttaa acgcatcaga gccatcagac tttcgcgcac cagtaactgc
      661 atttttaatt gcagttcatc actaagaatg ggtatttcgc gcgcagtgca acgggaaaag
      721 gacaaaaagc ccagcgcccg gtttggcagc attaaatact gagtgacacc gcggcgtaaa
      781 ccatgcgcgc gcgcagcttc ccataacggc tgtgcttcgc tgaataagtc atcattccac
      841 attaaatggc cctgactaaa gttttcaggg ttcagtacag gatcaattgc gagaaagttt
      901 tttgcctgat aataactaac ccacgcctca gggtaattgg tgtagaaagc cactttaggt
      961 cgcgtgaatg gtaccgggtg gcgaacacat aacgaatagt aatcgtactc cagctgctga
     1021 gcctgaagct ctatttcatg gtagacctct tctgcggcct ccatcctctg aaaacgcaac
     1081 agcatcgtgc gacgccagct gaaaaaatcc gtatcctgca tagtaaaccg caacgcccct
     1141 gagagtgagt atcatttata atgataattc aaactatcac ataaataaga tttatatata
     1201 atttatatta ttcaggcaat gaattacttt tgcaagccat cgcattctct tatgttatta
     1261 atgagttatg ctgatttgtt aagcagtttt atcacgtttg aagtggcgca ccgccccgac
     1321 aggtgaatcg tcggggctga ttttttctta ttattgcagc agaaacttct cgaggaactg
     1381 gcgggtgcga ggctgctcgg gatcggcaaa taacgctttt gcggccccct gctcgactat
     1441 ccgcccctgg tccataaaga tcgcccggtc cgcaacatcc cgggcaaagc tcatttcgtg
     1501 cgtcacaatc accatcgtgc gcttttcctg cgccagctgg cggatggtgt tcaggacttc
     1561 gcccaccagc tctggatcca gcgccgacgt tggctcgtca aacaaaatca cttcaggacg
     1621 catagccagc gctcgcgcaa tcgcgacacg ctgctgttga ccgccagaca aacgacgtgg
     1681 atagctggtt tctttacctg ccagcccaac tttcgccagc agctcgcgag cgcgcgccgt
     1741 ggcctcttct ttcggttcgc ctttgacgat caccggccct tcaataatgt tttccagcac
     1801 cgtacgatgc ggaaacaaat taaagttctg gaagacaaac ccgacgtgct gacgcaactg
     1861 gcgaatcagc gatttttgct gacttaatga acgtgcagta tcaatggtga tatcgccaac
     1921 ggtgatggtt cccgcttccg gttgttccag cagatttatg ctgcgtagca acgtggtttt
     1981 gccggaacca cttggaccga taattgccac cacttcgcca ggcttcacct caaggtcgat
     2041 accgtgcagc accgtctgac cgtggaattt tttcaccagg ttcttaactt caatggcact
     2101 cattttggtt ctctctcctg gcgattaagt tgattctcaa aatggttctg caacgtcgat
     2161 aatactgtcg ccatgatcca gtagatgagc gaagccgcca ggtacatggt gaacacctcc
     2221 agcgtgcgcg aggtaatcaa ctgcgcctga cggaacagct ccggcacctg gattgtcgcg
     2281 gccagcgacg tatcttttac caggctgata aagctgttcg acagcggcgg caacgccaca
     2341 cgcgccgcct gtggcaaaat cgcgcgacgc atggtctgcc acggcgtcat accaatactg
     2401 gcagcagctt cccactgacc tttatcaata gaagaaatgg cggcgcgcag tgtttcggcg
     2461 gcataggcgg cagtgtttag cgacagacca atcattgctg acggaatggg atctaattcg
     2521 atgccaaact gcggcaggcc gtaatagatc ataaacagtt gtgcgatgag tggcgtacca
     2581 cgaaaaatag agatataaaa acgcgccagc cagcgcacgg gccagattgg cgaaaggcgc
     2641 atcagcgcga gaataaaccc gagcagtaag ccaaaaaaca tgccgccgat actgagttgc
     2701 agcgtatacc cagccccttt cagcaggaac ggcaaggaat caataaccag ttgtatactt
     2761 tcttgcatga gcgtttccgg agctaaacgt ggggatgata ggcgaacagc gcaggcgcgc
     2821 cgccggtatg aataaacaga atcggccctt catctttgaa gcgtttctga ctgataccgt
     2881 caatcagccc cgccattgct tttccggtat acacaggatc aagcagaatg ccttccagcc
     2941 gcgccagcaa tttcactgct tccatgcctt cgtcgttcgg cacgccgtag ccaggtgcaa
     3001 aatagtcatc ccagagtaaa atttccgctg atgcggtcag ctccagttct ttcgcaatcg
     3061 cctgttggag gttaaccact ttcggcaatt gatcggcaac ggaacgcgac acggtcacgc
     3121 caatcagttc gctttcaggc atcaggtgtt ccagcccaac agccagtccg gcgtgagttc
     3181 cggcactgcc cgacgcgacc accaccgacg aaatattaac tgccccttca cactgttgcg
     3241 caatttccag cgcactctcc acataaccca gcgcgcccaa agcattagaa ccgccaaccg
     3301 gaatgacata cgggcgaaag ccttgtgctt cgactcgcgt cgccagctct tccagttggg
     3361 cattgggatc ggtcagtgcg tcgcacattt caatctgggt attgaacaga tccagcaaca
     3421 aacgattgcc gttggttaaa tagttttctg cggttgtgcc aataggattt tccagcagcg
     3481 ccacgcagtg cagaccgatt ttcgccgcaa ctgcggcagt ctggcgcaca tggttagact
     3541 ggatcgcccc ggcagtaatc agcgtatcgg caccttcacg cagagcatct gccgcgagaa
     3601 attccagctt acgtaattta ttgccgccca ttgccatggg agtgacgtca tcccgtttga
     3661 tgaaaatttc ccgtccaaga taatcagaaa agcgcggcag atattcgagc ggcgttggcg
     3721 cgccgataaa ctccagccgt ggaaaacggg ttaaattatg cagtggcata acagcctccg
     3781 atgtgtgttg ttgtgatttt cttattatgc acgctgaaaa cgcgtaaata aaaaaggcgc
     3841 tagtgaaagc gccctttttt gtcattatgc tgattatttg gtcacatcag caccaaacca
     3901 tttttcggaa agggcttgca gagtgccatc tttttgcatt tccgcaattg catcattcac
     3961 cgctttcagc aggtcttcat ttcctttacg cagcgccaca ccagactcct gacgggagaa
     4021 tgcttcaccg gttactgcca gcgtatcgtt ggttttcttc accagatcca gcgccgccag
     4081 acggtcaacg aggatcgcat cgatacggcc tacgcgcaga tcctgatatt tggtcgggtc
     4141 atcatcatag gtacgcacat cgacgccctg aacattctga cgcagccact cttcatagtt
     4201 ggtgcccaga ccgacaccca cttttttgcc tttcagatca tcagccgttt taatggtgcc
     4261 ttcgttacct tttttcacca gcgcctgaat accggaaatg gtgtacgggg tagagaaatc
     4321 gtattttttc ttgcgctcgt cagaaatggt gacctggtta atcaccacat caatgcgttt
     4381 agagtccagc gacgccagca taccgtccca tttagtcggt tttagtgacg cctcaacgcc
     4441 aagatgtttt gccagctgtt gggcaaattc cacttcaaaa ccggttaatt tgccgtcatc
     4501 cccctgaaaa ctgaacggcg gataagttcc ttccagccct accagcagcg tgccgcgctc
     4561 tttgacttta ttaagcagac cttcatctgc aaaactttta acgctcatac ccgcaaccag
     4621 cgccacggcc atcacaccca tcaatgcctg acgtcccaga tgtgctaatt tcatatttac
     4681 cccgaatgtt gttatgtctg tttgcagtgt agagccattg tttgtaaaca caaaaacaac
     4741 tccgctacat cttattctta tttaatatat atcagaagca ggcagactgg aggatttctg
     4801 cacaagtccg gtacacgttt gacgctgata gtgttgatac ttccgtaacg caatgcggta
     4861 attgttggtg ctggtggtgg gcagccacgg ggcgaggatt tcatcaagga aaccgtcctg
     4921 cagctgatcg agggaaatat tttgctcgtg tagatgatta cccaaacggc gcagacgaac
     4981 gacatattca cgcaccgtac ctgggctcat ttcagtttgc tcaaaaagaa attgcttaaa
     5041 gccgataata tcgaaaaaat cactctgcgt tttgcaatgt aaatcaccgc aaaatcggca
     5101 caatgccgcc catgattttt gttccacttg ccagccattt tcatcaagca gcatgtccag
     5161 gcgggaaatc tcgattttat tcactatttt gccgtcgcga acgagggtaa tgcgatcgag
     5221 taatttacgg caatgcgcgc aatgggtctg gctgtgttta aagtctttaa gatagcggct
     5281 taatggccgt cttttcaggt gctgcaccat cattaagaac tcctggtagt caaaattaaa
     5341 gtgcggcatt tactgacgtt ataacttacc cagtttagtg cgtaaccgtt taatggcctg
     5401 gctgtgtaac tgactgaccc gcgattcccc gacctccagc accgcgccaa tctctttgag
     5461 attcagctct tcctggtaat agagggttaa taccagtttt tcgcgctccg gcaacgtttc
     5521 gatagcttcc atcacccgct ggcgcagatt actgtccagc agttgttgta gcgggttttc
     5581 tcgctgatga tcatcagtaa ccagttcgat gctatcgccg tgctcttcgc gccactcatc
     5641 gtaggagaag agctggctgt tattggtgtc gagcaacatt tggcgatagt cggcaatatc
     5701 gatccctaaa cgttccgcta cctcagtttc cgtggcgttg cggccaagtt cctgctccag
     5761 ttgccctatt gcctgtgcca cttcacgcgc gttgcgtcgc acgctgcgcg gcacccagtc
     5821 acggctgcga agttcatcca gcatagcgcc acggatacgc tgcactgcgt aagttgtaaa
     5881 tgccgttcct tgtagggcgt catagcgttc gacggcattg agtaacccaa tgccgcccgc
     5941 ctgtagcaga tcgtcaagtt ccacgctcgc gggcagtcga acctgcaggc gcaatgcttc
     6001 gtgacgcacc agcgggacat aacgctgcca cagcgagtgt ttatccatta caccttcagc
     6061 ggtatagagt gaattcacga taaacagccc tgcgttatat gagttatcgg catgattatc
     6121 cgtttctgca gggtttttaa tcggacgatt agtgggtgaa atgaggggtt atttgggggt
     6181 taccggtaaa ttgcgggcag aaaaaacccc gccgttggcg gggaagcacg ttgctgacaa
     6241 attaccattc atgttgccgg atgcggcgta aacgccttat ccggcctaca aaatccgcaa
     6301 attcaataaa ttgcaattca ccttgtaggc ctgataagcg cagcgcatca ggcaatttgg
     6361 cgttgccgtc agtctcagtt aatcaggtta cgacgattaa ccctgcagca gagacagaac
     6421 ctgctgcggt acctggttag cttttgccag cacggagtta ccggcctgct ggatgatctg
     6481 cgctttcgac atgttggaca cttcggtcgc atagtcggcg tcctgaatac gggactgcgc
     6541 ttcggacagg ttggtagtgg tgttgttcag gttggtgacc gcggaatcca gacggttttg
     6601 caccgcaccg agggaggagc ggaatttgtc gatggagctg atggcttcgt ccagagcttt
     6661 cagtggatcg gcggtggttg cagctttagt ttcagcgtca gtagtaaaat taccatccgc
     6721 tgaaacatag actgctttac ctgaaccatt ggtcacagtt ccgtcttttt gcaaaaacag
     6781 attggttgta ttatcggtag tcagtgaacc atcagctttg gtgtaagcgt cttcaaaaac
     6841 tgtacctgtg tattgttgta ctataccttt actatcaact ttaatatcgc ctgcgctggt
     6901 accagtagct ccaacatcaa ctgtgaatcc tgtggactta gttgccgatt gaagagactc
     6961 agcactcatt cttgcatttg ctacagtgat tgcaccagaa gtagtagcat cagtaccgcc
     7021 atccagagtg aaggttgttt catcttcagt tgtaattacc gtagatacgg cacccggtgt
     7081 gcctgttgta tgccagtttt tagtcagtgc atcaagagtg gctgctttga cagtcccagc
     7141 attgttttga gtgaggttac catctacgtc aataaatagt tctgaaccat ctttcgcggt
     7201 aattttaccg tcgctagcca atacaacgtc aattgacgta gaaccaattt taacgtttaa
     7261 gttagcagta tcgcccgctt ttggtgtcag gaaggattgt aactgagcac tgttactatc
     7321 accagaattt gtaccagctg caatagttgc actatatgta aaatcacctg tagcgctgtt
     7381 gtatgtataa tctgtagctg ttggcgatcc aaaaccattt gcaattgtgg cattaacttt
     7441 tgcgttatta gccaaactcg caattacatc tgcagctgta gatgttttca ggcctgcgct
     7501 aacagtatag gtaactgtac cattagcatc agcagtacct ggagccaaga gttgagctgc
     7561 tgccaaatca gatttagtcg ctgcagtatt agccacagcc ccgccaccat tcacattaaa
     7621 cccactcagc cccagcgtat cagagtcaat tttcttcaga tcaatagtga tagtctggcc
     7681 gtcattcgca ccaacctgaa ttttcattga accgtctttc gccagtacgt tcacgccgtt
     7741 gaactgggtc tggccagata cgcggtcaat ttcgtccaga cgggatttga tttcgtcctg
     7801 aatggagtcc agatccgaat cggagttagt cccggtagaa gcctgaaccg tcagttcacg
     7861 gatacgctgt aagttgttgt tgatttcgga cagcgcgcct tcggtggtct gtgcaacgga
     7921 gataccgtcg ttggcgttac gtgcagcttg agtcagacct ttaatgttag aagtaaaacg
     7981 gttagcaatc gcctgacctg cggcgtcatc cttcgcgctg ttaatacgca agccagaaga
     8041 cagacgctcg atagaactcg acagcgcaga ctggttcttg ttgatattat tttgagtgat
     8101 cagcgagagg ctgttggtat taatgacttg tgccatgatt cgttatccta tattgcaagt
     8161 cgttgattac gtattgggtt tccacccgtc ggctcaatcg ccgtcaaccc tgttatcgtc
     8221 tgtcgtaaaa caacctttag aatttttttc acaaacagcc attttttgtt agtcgccgaa
     8281 atactctttt ctctgcccct tattcccgct attaaaaaaa acaattaaac gtaaactttg
     8341 cgcaattcag gccgataacc ccggtattcg ttttacgtgt cgaaagataa aaggaaatcg
     8401 catggcaagt atttcatcgc tgggagtcgg gtcaggtctg gatttaagtt caatccttga
     8461 tagcctcacc gccgcgcaaa aagcgacgct aacccctatt tcaaatcagc aatcgtcgtt
     8521 taccgctaaa cttagcgcct acggtacgct gaaaagcgcg ctgacgactt tccagaccgc
     8581 caatactgca ttgtctaaag ccgatctttt ttccgccacc agcactacca gcagcaccac
     8641 cgcgttcagt gccaccactg cgggtaacgc catcgccggg aaatacacca tcagcgtcac
     8701 ccatctggcg caggcgcaaa ccctgaccac gcgcaccacc agagacgata cgaaaacggc
     8761 gatcgccacc agcgacagta aactcatcat tcaacaaggc ggcgacaaag atccgattac
     8821 cattgatatc agcgcggcta actcatcgtt aagcgggatc cgtgatgcta tcaacaacgc
     8881 aaaagcgggc gtaagcgcaa gcatcattaa cgtgggtaac ggtgaatatc gtctgtcagt
     8941 cacatcaaat gacaccggcc ttgataatgc gatgacactc tcggtcagcg gtgatgatgc
     9001 gctacaaagt tttatgggct atgacgccag tgccagcagc aacggcatgg aggtctcggt
     9061 cgccgcccag aatgcgcagc tgacggtcaa caacgtcgcc attgagaaca gcagcaacac
     9121 catcagcgac gcgctggaaa acatcaccct gaacctgaac gatgtcacca cgggcaacca
     9181 gacgctaacc atcactcagg acacctccaa agcgcaaacg gcgattaaag actgggtgaa
     9241 tgcctataac tcgctaatag ataccttcag cagcctgacc aaatacaccg ccgtagatgc
     9301 gggagctgat agccagagtt ctagcaatgg cgcactgctc ggcgactcca cgttgcggac
     9361 gattcagacg cagttgaaat cgatgctgag taataccgtt agttcttcca actataaaac
     9421 gttggcgcag attggtatca cgaccgatcc cagcgatggc aaactggaac tggatgccga
     9481 caaactcacc gctgcactga aaaaagatgc cagcggcgta ggtgcattga ttgttggcga
     9541 tggtaaaaaa accggcatca cgaccaccat cggcagcaac ctgaccagtt ggctttcgac
     9601 aacaggcatt attaaagccg ctaccgatgg cgttagtaag accctgaata agttaaccaa
     9661 agactacaac gccgccagcg atcgcattga tgcgcaggtc gcgcgctaca aagagcaatt
     9721 tacccaactg gacgttttaa tgacctcgtt aaacagcacc agcagctact taacgcagca
     9781 gttcgaaaac aacagtaatt ccaagtaatc aatattcatc gggagacagg tcatgtacgc
     9841 ggcaaaaggc acccaggcct atgcacaaat tggcgtcgaa agcgccgtaa tgagcgccag
     9901 ccagcaacag ctggtcacca tgctatttga tggagtgctg agcgcactgg ttagagcgcg
     9961 cctgtttatg caggacaaca atcagcaagg caaaggcgtc tctttgtcaa aagcgatcaa
    10021 catcattgag aacgggctgc gggtgagtct tgatgaagag agcaaagacg aactaaccca
    10081 aaacttgatt gctctttata gctatatggt caggcgcttg ctgcaagcca atttacgcaa
    10141 cgatgtctcc gcagtcgaag aagtggaagc attaatgcgc aatattgccg atgcctggaa
    10201 agagtcttta ctctcccctt ctttgattca ggacccagac tgatgaacaa tgcaccgcat
    10261 ttgtatttcg cctggcaaca actcgtcgaa aaaagtcagc tcatgttacg cctggcgaca
    10321 gaagaacaat gggacgaact catcgccagc gaaatggcgt atgtgaatgc ggtgcaggag
    10381 atcgcacacc tgactgaaga ggttgccccg tccaccacga tgcaggagca gctccgcccg
    10441 atgctgcgcc tgattctcga caacgaaagc aaggtaaagc agttattaca gattcggatg
    10501 gatgaactgg cgaaactggt cggtcagtca tcggtgcaaa aatcggtgtt aagtgcctat
    10561 ggcgatcagg gtggctttgt gctggctccg caggataacc tcttttgaat cttaaagagc
    10621 caatggctcg cgaataatct gattacggct acgcttctaa tgttcccctt gaatggagtc
    10681 gaagaatgcg taatcccacg ctgttacaat gttttcactg gtattacccg gaaggcggta
    10741 agctctggcc tgaactggcc gagcgcgccg acggttttaa tgatattggt atcaatatgg
    10801 tctggttgcc gcccgcctat aaaggcgcat cgggcgggta ctcggtcggc tacgactcct
    10861 atgatttatt tgatttaggc gagtttgatc agaaaggcag catccctact aaatatggcg
    10921 ataaagcaca actgctggcc gccattgacg ccctgaaacg taatgacatt gcggtgctgc
    10981 tggacgtggt agtcaaccac aaaatgggcg cggatgaaaa agaagctatt cgcgtgcaac
    11041 gtgtaaatgc tgatgaccgt acgcaaattg acgaagaaat cattgagtgc gaaggctgga
    11101 cgcgttacac cttccccgcg cgtgccgggc aatactcgca gtttatctgg gatttcaaat
    11161 gttttagcgg tatcgaccat atcgaaaacc ctgatgaaga tggcattttt aaaattgtta
    11221 acgactatac cggagaaggc tggaacgatc aggttgatga tgaactcggt aatttcgatt
    11281 atctgatggg tgagaatatc gattttcgca atcatgccgt gacggaagag attaaatact
    11341 gggcgcgctg ggtgatggaa caaacgcaat gcgacggctt tcgtcttgat gcggtcaaac
    11401 atattccggc atggttttat aaagagtgga tcgaacacgt acaggaagtt gcgccaaagc
    11461 cgctgtttat tgtggctgaa tactggtcgc atgaagtcga taaactgcaa acgtatattg
    11521 atcaggtgga aggcaaaacc atgctgtttg atgcgccgct gcagatgaaa ttccatgaag
    11581 catcgcgcat gggtcgcgac tacgacatga cgcagatctt cactgggacg ctggtggaag
    11641 ccgatccttt tcacgccgta accctcgttg ccaaccacga cacccaaccg ttgcaagccc
    11701 tcgaagcacc ggtcgaaccg tggtttaaac cgctggcgta tgccttaatt ttgttgcggg
    11761 aaaatggcgt tccttcggtg ttctatccgg acctctacgg tgcacattac gaagatgtcg
    11821 gtggtgacgg gcaaacctat ccgatagata tgccaataat cgaacagctt gatgagttaa
    11881 ttctcgcccg tcagcgtttc gcccacggtg tacagacgtt atttttcgac catccgaact
    11941 gcattgcctt tagccgcagt ggcaccgacg aatatcccgg ctgcgtggtg gtcatgtcga
    12001 acggggatga tggcgaaaaa accattcatc tgggagagaa ttacggcaat aaaacctggc
    12061 gtgattttct ggggaatcgg caagagagcg tagtgaccga cgaaaacggc gaagcgacct
    12121 tcttttgcaa cggcggcagc gtcagcgtgt gggttatcga agaggtgatt taaattcatc
    12181 cccggcggca agccggggag atttcattac ggcagtggcg tcggtaacgg cgctttatcc
    12241 agcgcggcgg cacactctgc cgtcgggcga tccacacgct ccatcgtcat accatcgtac
    12301 tcaatggtgt taccgtcacg ctcaacctcg tacagctcac gtttcacggt aacgttagtg
    12361 agatcatcag acatcaatgt caacttaccc ggcacggcaa tcacccgctg ccactgacgg
    12421 caatccaggg tatcgccctc tttagtgaca atcaggctgc ctatcgcttc cggactgacc
    12481 agcgcacttt gcggcccttt ggtctgccag tagcccgcca gccagtccgg cgcgggggtt
    12541 ttcacgacat tgttatagtt ttcgacttcg gcacagcccg ccagcaacat gagcgcgcct
    12601 gcaattgcga gttttttcat cattcgtcct gcatgagaag aaaagaggat tgtggcatta
    12661 aagccctgat gtcgccagcc tttccaggtt cagagagaaa tttgatctaa ctattatttt
    12721 tgtgaattat taccccgaaa ataaatttgc ttgatgtatg attcgcaact cttgtttcaa
    12781 accttctgag ttcagaggct acatacatgt catggcagca attcaaacac gcctggttga
    12841 ttaaattctg ggcccccatc cctgcggtca tcgcggcggg tattctctcc acttactact
    12901 ttggcatcac tggcaccttt tgggctgtca cgggtgaatt tacccgctgg ggcggtcagc
    12961 ttctgcagct gttcggcgtc catgctgaag agtggggtta ttttaaaatt atccatctgg
    13021 aaggatcgcc attaacccgc atcgacggca tgatgatcct cggtatgttt ggcggctgct
    13081 ttgctgcagc gctgtgggcc aacaatgtca aactacgcat gccacgcagc cgtatccgca
    13141 ttatgcaggc cattattggc ggcattatcg ctggttttgg cgcgcgtctg gcaatgggct
    13201 gtaacctggc ggcgttcttt accggcattc ctcagttctc gctgcatgcc tggttctttg
    13261 ccatcgccac tgccattggt tcatggtttg gcgcgcgctt taccctgctg cccatcttcc
    13321 gtattcccgt gaaaatgcag aaagtttctg ccgcctcacc gctgacgcaa aaaccggatc
    13381 aggcgcggcg tcgttttcgt ctcgggatgc tggtcttttt cggcatgctg ggctgggcgc
    13441 tgctcacggc gatgaaccaa ccaaaactgg ggctggcaat gctgtttggc gtcggctttg
    13501 gtttactgat tgaacgtgcg caaatctgct ttacttcggc gttccgcgat atgtggatca
    13561 ccggacgtac ccatatggcg aaagcaatca ttatcggtat ggcggtgagt gccatcggga
    13621 tcttcagtta cgtacagtta ggcgttgaac ctaaaatcat gtgggcggga ccaaatgcgg
    13681 taattggtgg tttacttttt ggttttggca tcgtgctggc aggcggctgc gaaaccggct
    13741 ggatgtaccg cgcggtagaa ggccaggtgc actactggtg ggtcggtctg ggcaacgtga
    13801 tcggctcaac gattctggcg tattactggg atgatttcgc tccggcgctg gccaccgact
    13861 gggacaaaat caacctgctg aaaacctttg gtccgatggg tggcctgctg gtgacatatt
    13921 tgctgttgtt tgcagctcta atgttgatta tcggctggga aaaacgcttc ttccgccgtg
    13981 cggcaccgca gattgctaag gagatcgcat gaaaaatatc gttcctgatt accgtcttga
    14041 tatggtgggt gaaccctgcc cttatccggc tgtcgcaacc cttgaggcga tgccgcagtt
    14101 aaaaaaaggg gagatcctgg aagtggtgag cgactgtccg cagtcgatca ataatattcc
    14161 actggatgcg cgtaatcacg gctatacggt gctggatatt cagcaagacg ggccgaccat
    14221 tcgttattta attcagaagt aatccctcat tcccgctgtg gcccacagcg ggaacatcgt
    14281 taccactcta tcacatagat tttacccgcc ccctctccta ttcttttcag tgtgctctca
    14341 ggaggtttct atgtgtggac gctttgccca atcccaaacg cgtgaagatt acctcgcgtt
    14401 tctcgcggaa gatattgaac gcgatattcc ctacgatccc gaacctattg gcagatacaa
    14461 cgtcgcgccg ggaaccaaag tccttctgct cagtgaacgt gatgaacacc ttcatctgga
    14521 tccggttttc tggggatatg ctcccggatg gtgggataaa ccgccgctga ttaacgcccg
    14581 cgtagaaact gccgcaacca gtcgcatgtt taaaccgctc tggcaacacg gtcgggcaat
    14641 ctgttttgcc gatggctggt ttgagtggaa aaaagaaggc gacaaaaaac agccttattt
    14701 tatctatcgc gctgacggac aacctgtttt tatggccgcg ataggtagca caccgtttga
    14761 gcgtggtgac gaagccgaag gatttttgat cgtcactgcg gcggcagatc aaggtctggt
    14821 agatattcat gaccgccgcc cgctggttct gtcgccagaa gctgtacggg aatggatgcg
    14881 gcaagaggtt ggcggtaaag aagcctcaga aattgctgcc agtggctgtg tgccagcaaa
    14941 ccagttcacc tggcacccgg tatcgtgcgc ggtgggtaat gttaaaaacc agggagcggc
    15001 gttaattcaa cctgtttgat aagcctggag attattgatc aacaatactg cgtcataaga
    15061 aatctctatt agacaaagat ttcagtacct gttggcatat tgcaaaaata acaccaatac
    15121 ggaatcgtca tgttcacgat taaaacagat gatctcaccc atccagcagt gcaagcatta
    15181 gtggcttacc atatttccgg catgctgcag cagtctcccc ccgaaagtag tcatgcttta
    15241 gatgtgcaaa aattacgtaa cccgaaagtg acattctggt cagtatggga aggcgaacaa
    15301 ctcgcaggaa ttggtgcgct gaagttgctg gatgataaac atggcgaact gaaatcaatg
    15361 cgtaccgcgc caaattattt acgtcggggt gtcgccagtc tgattttacg ccacattttg
    15421 caggtcgccc atgacagatg ccttcatcgc ctgagcttag aaacgggtac acaggctgga
    15481 tttacggcct gccatcaact ttatttgaaa catggattcg ttgattgcga accgtttgct
    15541 gattatcaac ttgatccgca cagtcgattt ttgtcattga cgctatgcga aaataatgaa
    15601 ttgccatgag ccagacgcag cacattcttg cattcgacgt gctgcgcctt tatttattat
    15661 cagccgcaaa tgtcttgtcc ttagacgctc tttccacatg cgccacaaga aacctctatt
    15721 ccagtgacac aattatgcct gatt
//
LOCUS       DAZFLD010000114        15707 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.115, whole genome shotgun sequence.
ACCESSION   DAZFLD010000114 DAZFLD010000000
VERSION     DAZFLD010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15707
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.115"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(65..1954)
                     /gene="deaD"
                     /locus_tag="ACHKBL_003023"
     CDS             complement(65..1954)
                     /gene="deaD"
                     /locus_tag="ACHKBL_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DeaD"
                     /protein_id="HFO5819866.1"
                     /translation="MAEFETTFADLGLKAPILEALNDLGYEKPSPIQAECIPHLLNGR
                     DVLGMAQTGSGKTAAFSLPLLQNLDPELKAPQILVLAPTRELAVQVAEAMTDFSKHMR
                     GVNVVALYGGQRYDVQLRALRQGPQIVVGTPGRLLDHLKRGTLDLSKLSGLVLDEADE
                     MLRMGFIEDVETIMAQIPEGHQTALFSATMPEAIRRITRRFMKEPQEVRIQSSVTTRP
                     DISQSYWTVWGMRKNEALVRFLEAEDFDAAIIFVRTKNATLEVAEALERNGYNSAALN
                     GDMNQALREQTLERLKDGRLDILIATDVAARGLDVERISLVVNYDIPMDSESYVHRIG
                     RTGRAGRAGRALLFVENRERRLLRNIERTMKLTIPEVELPNAELLGKRRLEKFAAKVQ
                     QQLESSDLDQYRALLSKIQPTAEGEELDLETLAAALLKMAQGERTLIVPPDAPMRPKR
                     EFRDRDDRGPRDRNDRGPRGDREDRPRRERRDVGDMQLYRIEVGRDDGVEVRHIVGAI
                     ANEGDISSRYIGNIKLFASHSTIELPKGMPGEVLQHFTRTRILNKPMNMQLLGDAQPH
                     TGGERRGGGRGFSGERREGGRNFSGERREGGRGDGRRFSGERREGRAPRRDDSTGRRR
                     FGGDA"
     gene            complement(1947..2027)
                     /gene="yrbN"
                     /locus_tag="ACHKBL_003024"
     CDS             complement(1947..2027)
                     /gene="yrbN"
                     /locus_tag="ACHKBL_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrbN"
                     /protein_id="HFO5819867.1"
                     /translation="MKIADQFHDELCRLAAINFEAHVLHG"
     gene            complement(2134..3018)
                     /gene="nlpI"
                     /locus_tag="ACHKBL_003025"
     CDS             complement(2134..3018)
                     /gene="nlpI"
                     /locus_tag="ACHKBL_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020802045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HFO5819868.1"
                     /translation="MKPFLRWCFVATALTLAGCSNTSWRKSEVLAVPLQPTLQQEVIL
                     ARMEQILASRALTDDERAQLLYERGVLYDSLGLRALARNDFSQALAIRPDMPEVFNYL
                     GIYLTQAGNFDAAYEAFDSVLELDPTYNYAHLNRGIALYYGGRDKLAQDDLLAFYQDD
                     PNDPFRSLWLYLAEQKLDEKQAKEVLKQHFEKSDKEQWGWNIVEFYLGNISEQTLMER
                     LKADATDNTSLAEHLSETNFYLGKYYLSLGDLDSATALFKLAVANNVHNFVEHRYALL
                     ELSLLGQDQDDLAESDQQ"
     gene            complement(3127..5262)
                     /gene="pnp"
                     /locus_tag="ACHKBL_003026"
     CDS             complement(3127..5262)
                     /gene="pnp"
                     /locus_tag="ACHKBL_003026"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HFO5819869.1"
                     /translation="MLNPIVRKFQYGQHTVTLETGMMARQATAAVMVSMDDTAVFVTV
                     VGQKKAKPGQDFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPIRPL
                     FPEGFVNEVQVIATVVSVNPQVNPDIVAMIGASAALSLSGIPFNGPIGAARVGYINDQ
                     YVLNPTQDELKESKLDLVVAGTEAAVLMVESEAELLSEDQMLGAVVFGHEQQQVVIQN
                     INELVKEAGKPRWDWQPEPVNEALNARVAALAEARLSDAYRITDKQERYAQVDVIKSE
                     TIATLLAEDETLDENELGEILHAIEKNVVRSRVLAGEPRIDGREKDMIRGLDVRTGVL
                     PRTHGSALFTRGETQALVTATLGTARDAQVLDELMGERTDTFLFHYNFPPYSVGETGM
                     VGSPKRREIGHGRLAKRGVLAVMPDMDKFPYTVRVVSEITESNGSSSMASVCGASLAL
                     MDAGVPIKAAVAGIAMGLVKEGDNYVVLSDILGDEDHLGDMDFKVAGSRDGISALQMD
                     IKIEGITKEIMQVALNQAKGARLHILGVMEQAINAPRGDISEFAPRIHTIKINPDKIK
                     DVIGKGGSVIRALTEETGTTIEIEDDGTVKIAATDGEKAKHAIRRIEEITAEIEVGRV
                     YNGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQ
                     GRIRLSIKEATEQSQPAAAPEAPAAEQGE"
     gene            complement(5509..5778)
                     /gene="rpsO"
                     /locus_tag="ACHKBL_003027"
     CDS             complement(5509..5778)
                     /gene="rpsO"
                     /locus_tag="ACHKBL_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HFO5819870.1"
                     /translation="MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFA
                     EHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTQLIERLGLRR"
     gene            complement(5927..6871)
                     /gene="truB"
                     /locus_tag="ACHKBL_003028"
     CDS             complement(5927..6871)
                     /gene="truB"
                     /locus_tag="ACHKBL_003028"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HFO5819871.1"
                     /translation="MSRPRRRGRDINGVLLLDKPQGMSSNDALQKVKRIYNANRAGHT
                     GALDPLATGMLPICLGEATKFSQYLLDSDKRYRVIARLGQRTDTSDADGQIVEERPVT
                     FSAEQLAAALDTFRGDIEQIPSMYSALKYQGKKLYEYARQGIEVPREARPITVYELLF
                     IRHEGNELELEIHCSKGTYIRTIIDDLGEKLGCGAHVIYLRRLAVSKYPVERMVTLEH
                     LRELVEQAEQQDIPAAELLDPLLMPMDSPASDYPVVNLPLTSSVYFKNGNPVRTSGAP
                     LEGLVRVTEGENGKFIGMGEIDDEGRVAPRRLVVEYPA"
     gene            complement(6871..7272)
                     /gene="rbfA"
                     /locus_tag="ACHKBL_003029"
     CDS             complement(6871..7272)
                     /gene="rbfA"
                     /locus_tag="ACHKBL_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HFO5819872.1"
                     /translation="MAKEFGRPQRVAQEMQKEIALILQREIKDPRLGMMTTVSGVEMS
                     RDLAYAKVYVTFLNDKDEDAVKAGIKALQEASGFIRSLLGKAMRLRIVPELTFFYDNS
                     LVEGMRMSNLVTSVVKHDEERRVNPDDSKED"
     gene            complement(7436..10108)
                     /gene="infB"
                     /locus_tag="ACHKBL_003030"
     CDS             complement(7436..10108)
                     /gene="infB"
                     /locus_tag="ACHKBL_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HFO5819873.1"
                     /translation="MTDVTIKTLAAERQTSVERLVQQFADAGIRKSADDSVSAQEKQT
                     LIDHLNQKNSGPDKLTLQRKTRSTLNIPGTGGKSKSVQIEVRKKRTFVKRDPQEAERL
                     AAEEQAQREAEEQARREAEESAKREAQQKAEREAAEQAKREAAEQAKREAAEKDKVSN
                     QQDDMTKNAQAEKARREQEAAELKRKAEEEARRKLEEEARRVAEEARRMAEENKWTDN
                     AEPTEDSSDYHVTTSQHARQAEDESDREVEGGRGRGRNAKAARPKKGNKHAESKADRE
                     EARAAVRGGKGGKRKGSSLQQGFQKPAQAVNRDVVIGETITVGELANKMAVKGSQVIK
                     AMMKLGAMATINQVIDQETAQLVAEEMGHKVILRRENELEEAVMSDRDTGAAAEPRAP
                     VVTIMGHVDHGKTSLLDYIRSTKVASGEAGGITQHIGAYHVETENGMITFLDTPGHAA
                     FTSMRARGAQATDIVVLVVAADDGVMPQTIEAIQHAKAAQVPVVVAVNKIDKPEADPD
                     RVKNELSQYGILPEEWGGESQFVHVSAKAGTGIDELLDAILLQAEVLELKAVRKGMAS
                     GAVIESFLDKGRGPVATVLVREGTLHKGDIVLCGFEYGRVRAMRNELGQEVLEAGPSI
                     PVEILGLSGVPAAGDEVTVVRDEKKAREVALYRQGKFREVKLARQQKSKLENMFANMT
                     EGEVHEVNIVLKADVQGSVEAISDSLLKLSTDEVKVKIIGSGVGGITETDATLAAASN
                     AILVGFNVRADASARKVIEAESLDLRYYSVIYNLIDEVKAAMSGMLSPELKQQIIGLA
                     EVRDVFKSPKFGAIAGCMVTEGVVKRHNPIRVLRDNVVIYEGELESLRRFKDDVNEVR
                     NGMECGIGVKNYNDVRTGDVIEVFEIIEIQRTIA"
     gene            complement(10133..11620)
                     /gene="nusA"
                     /locus_tag="ACHKBL_003031"
     CDS             complement(10133..11620)
                     /gene="nusA"
                     /locus_tag="ACHKBL_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HFO5819874.1"
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEID
                     VRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTF
                     DRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEA
                     VILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGE
                     EVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLW
                     DDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGW
                     ELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKEL
                     LEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGIDRDLAFKLAA
                     RGVCTLEDLAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEA"
     gene            complement(11648..12100)
                     /gene="rimP"
                     /locus_tag="ACHKBL_003032"
     CDS             complement(11648..12100)
                     /gene="rimP"
                     /locus_tag="ACHKBL_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HFO5819875.1"
                     /translation="MSTLEQKLTEMITAPVEALGFELVGIEFIRGRTSTLRIYIDSED
                     GINVDDCADVSHQVSAVLDVEDPITVAYNLEVSSPGLDRPLFTAEHYARFVGEEVTLV
                     LRMAVQNRRKWQGVIKAVDGEMITVTVEGKDEVFALSNIQKANLVPHF"
     gene            complement(12307..12383)
                     /locus_tag="ACHKBL_003033"
     tRNA            complement(12307..12383)
                     /locus_tag="ACHKBL_003033"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12347..12349),aa:Met,seq:cat)
     gene            12731..14074
                     /gene="argG"
                     /locus_tag="ACHKBL_003034"
     CDS             12731..14074
                     /gene="argG"
                     /locus_tag="ACHKBL_003034"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HFO5819876.1"
                     /translation="MTTILKHLPVGQRIGIAFSGGLDTSAALLWMRQKGAVPYAYTAN
                     LGQPDEEDYDAIPRRAMEYGAENARLIDCRKQLVAEGIAAIQCGAFHNTTGGLTYFNT
                     TPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNAELQIYKPWLDT
                     DFIDELGGRHEMSEFMIACGFDYKMSVEKAYSTDSNMLGATHEAKDLEYLNSSVKIVN
                     PIMGVKFWDESVKIPAEEVTVRFEQGHPVALNGKTFSDDVEMMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLLTGIHNEDTIEQYHAHGRQLGRLLYQG
                     RWFDSQALMLRDSLQRWVASQITGEVTLELRRGNDYSILNTVSENLTYKPERLTMEKG
                     DSVFSPDDRIGQLTMRNLDITDTREKLFGYAKTGLLSSSAASGVPQVENLENKGQ"
     gene            complement(14082..>15707)
                     /gene="yhbX"
                     /locus_tag="ACHKBL_003035"
     CDS             complement(14082..>15707)
                     /gene="yhbX"
                     /locus_tag="ACHKBL_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFO5819877.1"
                     /translation="MTVFNKFVRSFKSHWLLYLCVIVFGITNLVASSGAHMVQRLLFF
                     VLTILVVKRISSLPLRLLVAAPFVLLTAADMSISLYSWCTFGTTFNDGFAISVLQSDP
                     DEVVKMLGMYIPYLCAFAFLSLLFLAVIIKYDVSLPTKKVTGILLLIVISGSLFSACQ
                     FAYKDAKNKKAFSPYILASRFATYTPFFNLNYFALAAKEHQRLLSIANTVPYFQLSVR
                     DTGIDTYVLIVGESVRVDNMSLYGYTRSTTPQVEAQRKQIKLFNQAISGAPYTALSVP
                     LSLTADSVLSHDIHNYPDNIINMANQAGFQTFWLSSQSAFRQNGTAVTSIAMRAMETV
                     YVRGFDELLLPHLSQALQQNTQQKKLIVLHLNGSHEPACSAYPQSSAVFQPQDDQDAC
                     YDNSIHYTDSLLGQVFELLKDRRASVMYFADHGLERDPTKKNVYFHGGREASQQAYHV
                     PMFIWYSPVLGDGVDRTTENNIFSTAYNNYLINAWMGVTKPEQPKTLEEVIAHYKGDS
                     RVVDANHDVFDYVMLRKEFTEDKQGNPTPEGQG"
BASE COUNT         3727 a         4260 c         3784 g         3936 t
ORIGIN      
        1 cccgatggta aaaatcgggg ctgtatatat tattttacag attgtgttcg ctgttcagcg
       61 atgattacgc atcaccaccg aaacgacgac gaccggtaga gtcatcacga cgcggagcgc
      121 ggccttcacg acgttcgccg ctaaaacgac gaccatcacc acgaccacct tcacggcgtt
      181 caccgctgaa gttacgaccg ccttcacgac gttcgccact gaaaccacga ccaccgccac
      241 gacgctcacc gccagtatgc ggctgtgcat cgcccagtaa ctgcatgttc atcggcttgt
      301 tgagaatgcg agtgcgcgta aagtgttgca gcacttcacc cggcatacct ttcggcagtt
      361 cgatggtgga gtgagaagca aacagcttga tgttaccaat gtaacggctg ctgatgtcgc
      421 cttcgttagc aatcgcacca acgatatgac gaacttcaac accatcatcg cggcccactt
      481 caatgcggta cagctgcata tcgccaacat cacgacgttc acgacgcgga cgatcttcac
      541 ggtcaccacg cgggccacgg tcgttacgat cgcgcggacc acggtcatca cggtcacgga
      601 attcacgttt cggacgcatc ggcgcatctg gcggaacaat cagagtacgt tcaccctgtg
      661 ccattttcag cagtgcggcg gccagagttt cgagatccag ctcttcacct tctgccgtcg
      721 gctgaatttt gctcagcagt gcacggtatt gatccagatc gctgctttcc agctgctgct
      781 gtactttagc ggcgaatttt tccagacgac gtttgcccag cagttctgcg ttcggcagtt
      841 caacttccgg aatggtcagc ttcatggtgc gttcaatgtt acgcagcaga cgacgctcac
      901 ggttctcaac gaacagcagc gcacggccag cacggcccgc acgaccagta cgaccgatac
      961 ggtgaacgta agactcagaa tccatcggga tatcgtagtt aactaccagg ctgatacgct
     1021 caacgtccag gccacgggcc gcaacgtcgg tcgcaatcag gatgtccaga cgaccatctt
     1081 tcagtcgttc cagtgtctgc tcacgcagcg cctggttcat gtcaccgttc agcgcggcgc
     1141 tgttgtagcc gttacgctca agggcttctg ccacttccag agtcgcgttt ttggtacgaa
     1201 cgaagataat cgccgcatca aaatcttccg cttccaggaa acgtaccagt gcttcgtttt
     1261 tacgcatacc ccagacagtc cagtagctct ggctgatgtc aggacgggta gtcacgctgg
     1321 actgaatgcg cacttcctgc ggctctttca taaagcggcg ggtaatgcga cgaatcgctt
     1381 ccggcatggt tgcagagaac agagcggtct gatgaccttc cgggatctgc gccataatgg
     1441 tttcaacgtc ttcgatgaag cccatgcgca gcatttcgtc agcttcatcc agaaccagac
     1501 cgctcagttt agagaggtcc agagtgccac gtttcaggtg gtccagcaga cggcccggag
     1561 taccgacaac gatctgcggc ccctgacgca gggcgcgtaa ttgcacgtca taacgttggc
     1621 cgccgtacag ggcaaccacg tttacgccgc gcatgtgttt agagaaatcc gtcattgctt
     1681 cagcaacctg taccgccagt tcgcgggtcg gtgccagcac cagaatctgt ggtgctttca
     1741 gctcaggatc aagattctgc agcagaggta aagagaatgc tgcagttttt ccgctccccg
     1801 tctgggccat acccagaacg tcgcggccat tcagcagatg tggaatacac tctgcctgaa
     1861 ttggagatgg tttttcgtaa cccagatcgt taagggcttc aaggatagga gccttcaggc
     1921 ccagatctgc aaaagtggtt tcgaattcag ccatgtagta cgtgtgcctc aaaattaatg
     1981 gcggccagtc tacataactc atcatgaaat tgatcagcaa ttttcattga aaagtgtgaa
     2041 ccggctcaaa gtaggtgtat taacgaacaa caacgccctc acccgttaag gtgatggcaa
     2101 tcaaaaaaga ttacgggctg atgtgtacgt cagctattgc tggtccgatt ctgccaggtc
     2161 atcttggtcc tggcccagga gcgataattc caacaatgcg tatcggtgct caacaaagtt
     2221 atgaacgttg ttggcaaccg ccagtttgaa cagtgccgtg gcgctgtcca aatcccccag
     2281 acttaggtag tacttaccta aatagaagtt ggtttcactg agatgctcag cgagcgaggt
     2341 gttatccgtt gcgtccgcct tgagcctttc cattaacgtt tgttcgctaa tgttgcccag
     2401 gtagaactcg acaatgttcc atccccactg ttccttatcc gatttttcga agtgctgttt
     2461 caacacttct ttagcctgct tctcatcgag cttctgctcg gcgagataaa gccacagact
     2521 acggaaagga tcattgggat cgtcttgata aaacgccagc agatcatctt gcgctaactt
     2581 gtcacgaccg ccgtaatata atgcgatccc gcgattcaag tgcgcgtagt tgtaagttgg
     2641 atcaagctca agtacagaat caaacgcttc ataggcagca tcaaaattgc ctgcctgcgt
     2701 taaatatatg cctaagtaat tgaatacttc aggcatatcc ggtcggattg ccagcgcttg
     2761 cgaaaaatcg ttacgcgcta atgccctcag accgagacta tcatacaaca ctccgcgctc
     2821 atataaaagc tgtgcgcgtt cgtcatcggt taaagcccga ctggcaagga tttgttccat
     2881 acgtgccaga atcacttcct gctgtaaagt cggttgcaat ggtaccgcga ggacttcact
     2941 tttacgccag gaagtattac tgcatcctgc aagcgtaagt gctgtcgcaa cgaaacacca
     3001 gcgcaaaaaa ggcttcattt cccactcccg aagaccacgg ttgaatgaac gtcctgttcc
     3061 cggttgctaa caaggcgtcc tgcccggtta aaagcccccc gccgcagcgg agggcaaatg
     3121 gcaaccttac tcgccctgtt cagcagccgg agcttccggt gctgcagcag gttgagactg
     3181 ctcagtcgct tctttaatgc tcagacggat acggccctgg cgatcaactt ccagaacttt
     3241 caccggtact tcctgaccca tctgcaggta atcggtcact ttctcaacgc gtttgtcagc
     3301 gatctgagag atgtgtacca gaccttcttt accgccgccg atggcaacaa atgcgccaaa
     3361 gtcaacgata cgggtcactt taccattgta gacgcggccc acttcgattt ctgcagtgat
     3421 ctcttcgata cgacgaatag catgtttcgc tttctcgccg tcggtcgctg cgattttcac
     3481 agtaccgtca tcttcgattt cgatggtggt gccagtttct tcggtcagag cacggataac
     3541 agaaccgcct ttaccgataa cgtctttgat cttgtccggg ttgatcttga tggtatggat
     3601 acgcggagcg aactcagaga tatcgccgcg cggcgcgttg atcgcctgtt ccattacgcc
     3661 caggatatgc agacgcgcac ctttagcctg gttcagcgca acctgcatga tctctttggt
     3721 gataccttca attttgatat ccatctgcaa tgcagagata ccgtcacggg aacccgcaac
     3781 tttgaagtcc atatcgccca ggtgatcttc gtcgcccaaa atgtcagaca gtacaacgta
     3841 gttgtcgcct tctttcacca gacccattgc gatacccgca acggcagctt tgatcggcac
     3901 acctgcgtcc atcagcgcca gagacgcgcc gcacacggaa gccatagaag aggaaccgtt
     3961 ggattcggtg atttcagaca caacacgtac ggtatacggg aatttgtcca tatccggcat
     4021 gactgccagc acgccgcgct tcgccagacg accgtgacca atttcacgac gcttcggaga
     4081 accgaccatg ccggtttcgc ctacagagta cggagggaag ttgtagtgga acaggaaggt
     4141 gtcagtacgt tcgcccatca gttcatcaag aacctgcgcg tcacgtgcag tacctagcgt
     4201 tgcggtaacc agcgcctgag tttcaccacg ggtgaacagc gcagaaccgt gagtacgcgg
     4261 cagcacacca gtacgcacat ccagaccacg gatcatatct ttttcacgac cgtcgatacg
     4321 cggttcgcct gccagtacgc ggctacgaac aacatttttc tcgatagcgt gcagaatttc
     4381 acccagttcg ttttcgtcca gggtttcgtc ttcagcaagc agtgtcgcga tggtttcaga
     4441 tttgatgaca tcaacctgcg cataacgctc ttgtttgtcg gtgatgcggt aagcatcgct
     4501 cagacgagct tcagccagtg cagcaacgcg cgcgttcagc gcttcgttta ccggctccgg
     4561 ctgccagtcc caacgcggtt taccggcttc tttcaccagt tcattgatgt tctgaataac
     4621 aacctgctgt tgttcatgac cgaagactac tgcgcccagc atctggtctt cgctcagcag
     4681 ttcagcttca gattcaacca tcagtacagc ggcttcagta cccgcaacaa ccagatccag
     4741 tttgctctct ttcaactcgt cctgagtcgg gttcagtacg tactggtcat tgatgtaacc
     4801 tacacgggca gcaccaatcg ggccattgaa cggaatacca gacagagaca gggctgcgga
     4861 agcaccaatc atcgcgacga tatccgggtt aacctgcggg ttaacagaaa ccacggtggc
     4921 gataacctga acttcgttga cgaagccttc cgggaacagc gggcgaatcg ggcggtcaat
     4981 cagacgcgcg atcagggttt cgccttcgct tgggcggcct tcacgacgga agaagctacc
     5041 cgggatacga ccagcagcgt aggtacgctc ctgatagtta acggtcagcg ggaagaagtc
     5101 ctgacctggt ttggcttttt tctgaccaac aacggtaacg aataccgcag tgtcatccat
     5161 gctaaccata acagcggcag tagcctgacg agccatcatg ccggtttcca gagtcacggt
     5221 gtgttggccg tactggaatt tacgaacgat cggattaagc aatgtaatat cctttctctt
     5281 tcttagacag taccttacgg cactggtgtt aatacccgat cttctgcgca tcctcgcgac
     5341 taatgacaac cctaacccag ctctatgtgg gtaaagcctc tcattagccg cgcgaacctc
     5401 tgcagcggaa gatcattcat agcaacaata cattagtttc cagtgaattg ctgccgtcag
     5461 cttgaaaaaa ggggccactc aggccccctt ttctgaaact cgcaagaatt agcgacgcag
     5521 acccaggcgc tcgatgagct gggtgtaacg tgctacgtct ttacgtttca ggtagtcgag
     5581 cagtttacga cgctgagaaa ccatgcgcag cagaccacga cggctgtggt gatctttttt
     5641 gtgctctgca aagtggccct gcaggtggtt gatctgtgca gtcagcagtg ctacctgaac
     5701 ttcggtagaa ccggtgtcgt ttgcgtcacg accaaactca gaaacgattt tagctgttgc
     5761 ttcagtactt agagacattt taaaactcca aagtatatag aatgaaagga cgccgatctc
     5821 taattcagcg atcccagtgt acgttacgca aagtgttaaa caatttacgc gacgttaagc
     5881 ggcagtattc tactcgtagc gacttgttat cgcaagacgg ttaacattac gccgggtatt
     5941 caaccaccag gcgacgaggc gcaacgcggc cttcatcgtc aatttcgccc ataccgataa
     6001 acttgccgtt ctcaccctct gtaacgcgaa ccagtccttc cagcggcgca ccggatgtac
     6061 gaactgggtt accatttttg aagtaaacag aagacgttaa cggaagattc accactggat
     6121 agtccgaagc tggactgtcc attggcatca gtaatggatc aagtaactcc gcagccggaa
     6181 tatcctgctg ttcggcttgc tcaaccagtt cacgcagatg ttccagggtc accatccgtt
     6241 caaccggata tttacttacc gccagacgac gcaagtaaat aacatgcgcg ccacaaccga
     6301 gtttttcgcc gagatcgtca atgatagtgc ggatataagt gccttttgag cagtgaattt
     6361 ccagctccag ctcattgcct tcatggcgaa taaacagcaa ttcataaacg gtaatcgggc
     6421 gcgcttcacg cggaacttca atgccctgac gcgcgtattc gtacagtttt ttgccctgat
     6481 atttcagtgc tgaatacatc gaagggatct gttcgatatc gccacggaag gtatccagcg
     6541 ccgccgccag ttgctctgca ctaaaggtta ccggacgttc ttcaacgatt tgtccgtcgg
     6601 catcagaagt atcggtacgc tgtccaagac gcgcaatgac ccgataacgt ttgtcggagt
     6661 ccagcagata ctgggaaaac ttcgtcgctt ccccgaggca aattggcaac atgccggtcg
     6721 ccagcgggtc cagcgcaccg gtatgcccgg cacggttggc gttataaatc cgtttcactt
     6781 tttgcagcgc atcgttgctg gacatcccct gcggtttatc cagcaacaaa acgccgttaa
     6841 tgtcgcgacc gcgacgacga ggacgactca ttagtcctcc ttgctgtcgt ccgggttaac
     6901 acgacgttct tcgtcatgtt tgaccacgct ggtcaccagg tttgacatgc gcatcccttc
     6961 aaccagagag ttgtcgtaga agaaggtcag ttccggcacg atacgcaggc gcatcgcttt
     7021 ccccagcagg ctgcggatga aaccagaagc ttcctgcagc gctttgatgc ccgctttaac
     7081 cgcgtcttca tctttgtcgt tgaggaacgt tacatatact ttggcatacg ccaggtcgcg
     7141 agacatttcg acaccggaaa cggtggtcat catgcccaga cgaggatctt taatttcacg
     7201 ctgcaggatg agagcgatct ctttttgcat ttcctgcgct acgcgctgcg ggcgaccaaa
     7261 ttctttcgcc ataataaatt ctcctgacaa aaaaggggct gttagcccct ttttaaaatt
     7321 aatttcaggt ggaagggctg ttcacgttgg cctgataaga cgcgccagcg tcgcatcagg
     7381 caatccatgc cggatgcggc gtaaacgcct tatcccgcat ggaaccctaa aaaccttaag
     7441 cgatggtacg ttggatttcg atgatttcga atacttcgat cacatcgcca gtgcggacgt
     7501 cgttgtagtt cttaacgccg ataccacatt ccataccgtt acggacttcg ttaacgtcat
     7561 ctttgaagcg gcgcagggac tccagctcgc cttcgtagat aaccacgttg tcgcgcagaa
     7621 cgcggatcgg gttgtgacgt ttaaccacac cttcggtaac catacaacct gcgatggcac
     7681 caaatttcgg tgatttgaat acgtcacgaa cttccgccag accgataatc tgttgtttca
     7741 gttccggaga cagcataccg ctcattgccg ctttcacttc gtcgatcagg ttatagatga
     7801 cggagtagta acgcagatcc aggctttccg cttcaatcac tttacgtgca gaggcatcag
     7861 cacgtacgtt gaagccaacc aggatggcat tggacgccgc agccagggta gcgtcggttt
     7921 cggtgatacc acctacgcca gaaccgatga tcttcacttt aacttcgtca gtagacagtt
     7981 tcagcaagga gtcggagata gcttccacag aaccctgtac gtctgccttc aggacgatat
     8041 tcacttcgtg aacttcgcct tcggtcatgt tggcgaacat gttctcgagt ttagatttct
     8101 gctgacgcgc cagtttaact tcgcggaatt taccctgacg atagagtgca acttcacgcg
     8161 ctttcttctc gtcacgtaca acggtaactt catcacccgc agccggaacg ccggacaggc
     8221 cgaggatttc caccggaatg gacggacccg cttccagcac ttcctgaccc agttcgttgc
     8281 gcatcgcacg aacacgaccg tattcgaagc cacacagaac gatatcgccc ttgtgcagag
     8341 taccttcacg taccagaacg gtagcaaccg gaccacgacc tttatcgagg aaggattcga
     8401 taaccgcacc gctcgccata cctttacgta ccgctttcag ttccagaact tccgcctgca
     8461 gcaggatagc gtccagcagc tcatcgatac cagtacccgc tttcgcagac acatgtacga
     8521 actggctttc accgccccac tcttccggca ggatgccgta ctgggagagt tcgttcttaa
     8581 cgcgatccgg atcggcttct ggtttatcga tcttgttcac tgcaaccacc accggaacct
     8641 gcgccgcttt cgcgtgctgg attgcttcga tggtctgcgg cattacgccg tcgtcggcag
     8701 caacaaccag gactacgatg tccgttgcct gcgcaccacg agcacgcatt gaagtaaacg
     8761 cggcgtgccc cggggtgtcc aggaaggtga tcatgccgtt ttcagtttca acgtggtatg
     8821 caccaatgtg ctgggtaatg ccgcccgctt cgccagaggc cactttcgtt gaacgaatgt
     8881 agtccagcag agaggtttta ccgtggtcaa cgtgacccat gatggtcaca accggcgcgc
     8941 gcggttcagc cgctgcaccc gtgtcacggt cgctcattac cgcttcttcc aactcgtttt
     9001 cacgacgcag gataactttg tggcccatct cttcagcaac cagctgtgcg gtttcctgat
     9061 cgataacctg gttgatggtt gccattgcgc ccagtttcat catcgctttg atgacctgag
     9121 agcctttaac cgccatcttg ttcgccagtt cgccaacggt gatggtttcg ccgatcacaa
     9181 cgtcacggtt aacggcctga gcaggcttct ggaagccttg ctgcagcgaa gaacctttac
     9241 gttttccgcc cttaccgcca cgtactgctg cgcgtgcttc ttcacgatca gcttttgatt
     9301 cagcgtgttt gttgcctttc ttcggacgcg cagctttcgc gttacgacca cggccacggc
     9361 cgccttcgac ttcacgatcg ctttcgtctt ctgcctggcg agcatgttga gaagtagtaa
     9421 cgtgataatc gctggaatct tcagtcggtt ccgcgttatc agtccatttg ttttcttccg
     9481 ccatacgacg tgcttcttca gcaacgcgac gtgcttcttc ttcgagttta cgacgcgctt
     9541 cttcttcggc tttacgcttg agctctgcag cttcctgctc acggcgggct ttttcagcct
     9601 gggcgttttt agtcatatcg tcttgttgat tgctcacttt gtctttttcc gcagcttcac
     9661 gtttcgcttg ttcagcagct tcacgcttag cttgttctgc ggcctcacgt tcagcttttt
     9721 gttgcgcctc acgtttggcc gattcttctg cctcacgacg ggcttgctct tccgcttcac
     9781 gctgcgcttg ctcttccgca gcaaggcgtt cagcctcttg cggatcgcgt ttcacaaagg
     9841 tgcgtttctt gcggacttcg atttgtaccg atttgctttt tccaccggta ccaggaatgt
     9901 taagggtgct gcgtgtttta cgttgcagcg tcaatttgtc cgggcctgaa tttttctgat
     9961 tcaggtggtc aatcaaagtc tgtttctctt gtgcagacac agagtcgtca gcagacttcc
    10021 ggatacctgc atcagcaaat tgctgtacca ggcgttccac ggaggtctgt cgctctgcgg
    10081 ccagcgtttt aatcgttaca tctgtcatgc tgttccttcc tgctacagtt tattacgctt
    10141 cgtcaccgaa ccagcaaata ttacgggcag ccataatcag tgctccggct ttttcgtcgg
    10201 tcaacccttc gatatcagcc agatcatcaa tgccctgttc ggcgagatct tccagcgtac
    10261 aaacgccacg ggcggccagt ttgaatgcca aatcacgatc tatcccttca aggttcagca
    10321 gatcgtcagc cggtttgtta tcaccgaggc tttcttcctg ggcctgtgca atggtggcca
    10381 gtgcattttt agcacgctcg cgcagtgctt caacggtcgg ctcatcaagg ccttcgattt
    10441 ccaacagctc tttcatcggc acataggcca gttcttccag cgtcgagaag ccttcttcta
    10501 ccagaacagt cgcgaagtct tcgtcgatgt cgagatattt ggtgaaggtg tcgatcgctg
    10561 cgtgcgcttc cgcctgatgc ttagcctgca ggtcgtcaac ggtcatcacg ttgagttccc
    10621 aaccgctcag ctgcgaagcc agacgcacgt tctgaccatt acggccaatc gcctgcgcca
    10681 ggttaccggc ttcaacggcg atatccatgg tgtgtttatc ttcatccacc acgatagaag
    10741 caacgtctgc cggtgccatt gcgttaatca cgaactgcgc cgggttatca tcccacagga
    10801 cgatatcgat acgctcgccg cccagttcag tagaaaccgc ctgaacacgc gcgccacgca
    10861 tacctacgca agcacctacc ggatcgatac gtttatcgtt ggttttcacc gcgattttcg
    10921 cacgagaacc cggatcgcga gccgctgctt taatttcaat cacttcttcg ccgatttccg
    10981 gcacttcaat gcggaacagt tcgatcagca tttccggctt ggaacgggtg acgaacagtt
    11041 gcgcgccacg cgcttccggg cgaacggaat agagcacgcc acgaacgcgg tcgccaggac
    11101 ggaagttttc acgcggcagc atatcttcgc gcaggatcac ggcttcagcg ttgttgccca
    11161 gatccagaga gatgttgtcg cggtttactt ttttcaccac gccggtgatg atttcacctt
    11221 cgtgttcacg gaactgatca accaccatcg cacgttcggc ttcacgcact ttctgcacga
    11281 taacctgttt tgccgtctgg gtggtgatac ggtcaaaggt aacagactca atctgatctt
    11341 caacgtaatc gcccaggttc aggctttcat cttcataacg tgcggcttca agggtgattt
    11401 ccttggtcgg ctgggtgact tcatcaacaa ctaaccagcg acggaaggtg tcaaaatcac
    11461 cgcttttgcg atcgatctgt acacggacgt cgatctcttg ctcatatttt ttctttgttg
    11521 ctgtcgccag cgcgctttcc aatgcttcga aaatcttctc gcgaggtagc gccttttcat
    11581 tggatacggc ttcaactaca gccaaaattt ctttgttcat cgcgggcttt tcacctcatc
    11641 cagactatta aaagtgggga accaggttcg ccttctggat attactcagc gcgaacactt
    11701 catctttacc ttcgacggta actgtgatca tttcaccgtc taccgctttg ataacgccct
    11761 gccatttacg acggttttgt accgccatgc ggagaaccag agtcacctct tctccgacga
    11821 aacgggcgta gtgttcagcc gtgaacagtg ggcgatcgag acccggtgag gagacttcca
    11881 ggttataagc aacggtgatg ggatcttcaa catccagcac agcacttacc tggtggctca
    11941 catcagcaca atcatcaaca ttgatgccat cttcactatc aatatagatg cgcagtgtgg
    12001 atgtgcgacc gcgaataaat tcgatgccaa ccagttcaaa acccagggcc tcaactggcg
    12061 cagtaatcat ctctgttaat ttttgctcta atgtggacaa gcccaccccc aagacataaa
    12121 aaaagggcct aaagcccagt tattctgtag tcagataaca aaaaaccccg ataaatcggg
    12181 gctttatata actgaaccct ataaccgcaa ctgcggtctg gagcactttc cagaaggatt
    12241 ttttcaaatc ccactacgaa ggccgaagtc ttcacagtat atttgaaaaa ggactctaag
    12301 ggaaagtggt tgcgggggcc ggatttgaac cgacgacctt cgggttatga gcccgacgag
    12361 ctaccaggct gctccacccc gcgcctgaaa cgtggcaaat tctactcgtt ttgggtaaaa
    12421 aatgcaaata ctgctgggat ttggtgtacc gagacgggac gtaaaatctg caggcattat
    12481 agtgatccac gccacatttt gtcaacgttt attgctaatc acgtgaatga atatccagtt
    12541 cactttcatt tgttgaatac ttttgccttc tcctgctctc ccttaagcgc attattttac
    12601 aaaaaacaca ctaaactctt cctgtctccg ataaaagatg attaaatgaa aactcattta
    12661 ttttgcataa aaattcagtg agagcggaaa tccaggctcc tcatcagtta attaagcagg
    12721 gtgttatttt atgacgacga ttctcaagca tctcccggta ggtcaacgta ttggtatcgc
    12781 tttttccggc ggtctggaca ccagtgccgc actgctgtgg atgcgacaaa agggagctgt
    12841 tccttatgca tatactgcaa atctgggcca gccagacgaa gaggattatg atgcgatccc
    12901 tcgtcgtgcc atggaatacg gcgcggagaa cgcacgtctg atcgactgcc gcaaacaact
    12961 ggtggccgaa ggtattgccg ctattcagtg tggcgcattt cataacacca ctggcggcct
    13021 gacctatttc aacacgacgc cgctgggccg cgccgtgact ggcaccatgc tggttgctgc
    13081 tatgaaagaa gatggcgtga atatctgggg tgacggtagc acctataaag gaaacgatat
    13141 cgaacgtttc tatcgttacg gtctgctgac caatgctgaa ctgcagattt acaaaccgtg
    13201 gcttgatact gactttattg atgaactggg cggtcgtcat gaaatgtctg aatttatgat
    13261 tgcctgcggt ttcgactaca aaatgtctgt cgaaaaagcc tactccacgg actccaacat
    13321 gctcggcgca acgcatgaag cgaaggatct ggaatacctc aactccagcg tcaaaatcgt
    13381 caacccgatt atgggcgtga agttctggga tgagagcgtg aagatcccgg cagaagaagt
    13441 cacagtacgc tttgagcaag gtcatccggt ggcgctgaac ggtaaaacct ttagcgacga
    13501 cgtagaaatg atgctggaag ctaaccgcat cggcggtcgt cacggcctgg gcatgagcga
    13561 ccagattgaa aaccgtatca tcgaagcaaa aagccgcggc atttatgaag cccctgggat
    13621 ggcactgctg cacattgcgt atgaacgttt gttgaccggt attcacaacg aagacaccat
    13681 tgagcagtat cacgcgcatg gtcgtcagtt gggccgtctg ctgtaccagg gacgttggtt
    13741 tgactcccag gcgctgatgc tgcgggactc tctgcaacgc tgggttgcca gccagatcac
    13801 tggtgaagtt actctggagc tgcgccgcgg gaacgattat tcaatcctga ataccgtctc
    13861 agagaacctg acctacaagc cagagcgtct gacgatggaa aaaggcgact cggtgttctc
    13921 gccagatgat cgtatcggtc aattgaccat gcgtaacctg gatatcaccg atacccgcga
    13981 gaaacttttc ggttatgcca aaactggcct cctctcctcc tctgccgctt caggcgtgcc
    14041 gcaggtggag aatctggaaa ataaaggcca gtaattcgac atcaaccctg cccttcaggg
    14101 gtggggttac cttgcttatc ctctgtaaac tcctttctga gcatcacata atcgaaaaca
    14161 tcatggtttg catccacaac ccgtgagtct cctttatagt gtgcaatcac ttcctccagc
    14221 gttttcggtt gttccggctt tgttaccccc atccacgcat taatcaggta attattgtaa
    14281 gctgtggaaa agatgttgtt ttccgttgtg cgatccacgc catccccaag aacggggcta
    14341 taccagataa acatcgggac atgatatgcc tgctggctgg cctccctgcc tccatgaaaa
    14401 tagacgttct tcttcgtagg gtcacgttcc aggccgtggt cggcaaaata catgaccgag
    14461 gcgcggcgat cttttaataa ttcaaaaacc tgacctagca aactatctgt gtaatgaatg
    14521 gagttgtcat agcaggcatc ctgatcgtcc tgcggttgaa acacggcgct ggattgcgga
    14581 taggcgctac aagccggttc atggcttcca tttaaatgaa gaacaatcag tttcttttgc
    14641 tgcgtatttt gctgtaatgc ttgcgataaa tgcggcaaca acaattcatc aaatcctctg
    14701 acataaactg tttccatggc gcgcatggcg atactggtaa ctgctgtacc attctgccga
    14761 aaagcggatt gcgagcttag ccagaaagtc tgaaatcctg cttgattagc catattaata
    14821 atgttgtccg ggtaattatg aatgtcatga ctcaaaacgg aatcagcagt taaagaaagg
    14881 ggaaccgaca gcgcagtgta aggtgcaccg cttattgctt gattaaacag tttgatctgt
    14941 tttctttgtg cttcgacttg cggtgtcgta gagcgtgtat atccatacaa agacatattg
    15001 tcgacgcgta cagactcccc cacaatcaac acgtaggtat caatacctgt atccctgact
    15061 gataattgaa aatacggcac cgtgtttgca attgagagta atctttgatg ctctttcgct
    15121 gctaaagcaa aataattgag attgaaaaac ggtgtatagg tagcaaatcg cgacgctagt
    15181 atatatggac tgaacgcttt tttatttttt gcatctttat aagcaaattg acaagcggaa
    15241 aataaactgc ccgaaatgac aatcagcaat aatattcctg tcactttttt cgtcggcaag
    15301 gaaacatcat attttattat tactgccaaa aaaagaaggg ataaaaaggc aaaagcacat
    15361 agataaggga tatacatccc cagcattttg acaacttcat ccggatcact ctgcagcaca
    15421 ctaatcgcaa atccatcatt gaaagttgta ccaaaggtac accatgaata gaggctaata
    15481 ctcatgtctg ccgctgtcag taacacaaat ggtgcggcaa caagcaggcg aagcggaagg
    15541 gatgatatac gtttaacaac caggatggtc agaacgaaga acagcaatcg ctgaaccata
    15601 tgcgctccgg aagaggcgac taagttcgta ataccaaaaa caataacaca aagatacaac
    15661 aaccaatgag atttaaaact tctaacaaat ttgttgaata ctgtcat
//
LOCUS       DAZFLD010000115        15698 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.116, whole genome shotgun sequence.
ACCESSION   DAZFLD010000115 DAZFLD010000000
VERSION     DAZFLD010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15698
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.116"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(138..350)
                     /gene="cspH"
                     /locus_tag="ACHKBL_003036"
     CDS             complement(138..350)
                     /gene="cspH"
                     /locus_tag="ACHKBL_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspH"
                     /protein_id="HFO5819878.1"
                     /translation="MSRKMTGIVKTFDRKSGKGFIIPSDGRKEVQVHISAFTPRDAEV
                     LIPGLRVEFCRVNGLRGPTAANVYLS"
     gene            636..848
                     /gene="cspG"
                     /locus_tag="ACHKBL_003037"
     CDS             636..848
                     /gene="cspG"
                     /locus_tag="ACHKBL_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspG"
                     /protein_id="HFO5819879.1"
                     /translation="MSNKMTGLVKWFNADKGFGFITPDDGSKDVFVHFTAIQSNEFRT
                     LNENQKVEFSIEQGQRGPAAANVVTL"
     gene            859..1047
                     /gene="ymcF"
                     /locus_tag="ACHKBL_003038"
     CDS             859..1047
                     /gene="ymcF"
                     /locus_tag="ACHKBL_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFO5819880.1"
                     /translation="MTPHLHFRCPCCHGSQYRTSAFDVTERNPLGAKCIFCKSTMITF
                     DNVALQIRTDHAPLDFTK"
     gene            1022..1252
                     /gene="ymcE"
                     /locus_tag="ACHKBL_003039"
     CDS             1022..1252
                     /gene="ymcE"
                     /locus_tag="ACHKBL_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmcE"
                     /protein_id="HFO5819881.1"
                     /translation="MRRWISQNNIRLPRGAFFISALFFFNAVCIVSDNLLIIESFGEM
                     AYNISYLTRVPGTNTLLACCCLSRPEEVNSEY"
     gene            1242..1415
                     /gene="gnsA"
                     /locus_tag="ACHKBL_003040"
     CDS             1242..1415
                     /gene="gnsA"
                     /locus_tag="ACHKBL_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, GnsA/GnsB family"
                     /protein_id="HFO5819882.1"
                     /translation="MNIEELKKQAETEIADFIAQKIAELNKNTGKEVSEIRFTAREKM
                     TGLESYDVKIKIM"
     gene            complement(1463..2536)
                     /gene="yccM"
                     /locus_tag="ACHKBL_003041"
     CDS             complement(1463..2536)
                     /gene="yccM"
                     /locus_tag="ACHKBL_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFO5819883.1"
                     /translation="MTEKRRTRWQRRPGTTGGKLPWNDWRNATTWRKATQLLLLAMNI
                     YIAITFWYWVRYYETAGSTTFVTRPGGIEGWLPIAGLMNLKYSLATGQLPSVHAAAML
                     LLVAFIVISLLLKKAFCSWLCPVGTLSELIGDLGNKLFGRQFVLPRWLDTPLRGVKYL
                     LLSFFLYIALLMPAQAIHYFMLSPYSVVMDVKMLNFFRHMGTATLISMIVLLIASLFI
                     RHAWCRYLCPYGALMGVVSLLSPFKIRRNAESCIDCGKCAKNCPSRIPVDKLIQVRTV
                     ECTGCMTCVESCPVASTLTFSLQKPAANKKAFALSGWLMTLLILGIMFAVIGYAMYAG
                     VWQSPVPEELYRRLIPQAPMIGH"
     gene            complement(2608..5352)
                     /gene="torS"
                     /locus_tag="ACHKBL_003042"
     CDS             complement(2608..5352)
                     /gene="torS"
                     /locus_tag="ACHKBL_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system sensor histidine
                     kinase/response regulator TorS"
                     /protein_id="HFO5819884.1"
                     /translation="MNLTLTRRLWMGFALMALLTLTSTLVGWYNLRFISQVEKDNTQA
                     LIPTMNMARQLSEASAWELFAAQNLTSADNEKMWQAQGRMLTAQSLKINALLQALREQ
                     DFDTTAIEQQEQEISRSLRQQGELVGQRLQLRQQQQQLSQQIVAAADEIARLAQGQAN
                     NAATSAGATQAGIYDLIEQHQRQAAESALDRLIDIDLEYVNQMNELRLSALRVQQMVM
                     NLGLEQIQKNAPTLEKQLNNAVKILQRRQIRIEDPGVRTQVATTLTTVSQYSDLLALY
                     QQDSEISNHLQTLAQNNIAQFAQFSSEVSQLVDTIELRNQHGLAHLEKASARGQYSLL
                     LLGIVSLCALILILWRVVYRSVTRPLAEQTQALQRLLDGDIDSPFPETAGVRELDTIG
                     RLMDAFRSSVHALNRHREQLAAQVKARTAELQELVIEHRQARAEAEKANQAKSAFLAA
                     MSHEIRTPLYGILGTAQLLADNPALNAQRDDLRAITDSGESLLTILNDILDYSAIEAG
                     GKNVSVSDEPFEPRPLLESTLQLMSGRVKGRPIRLATAIADDVPTALMGDPQRIRQVI
                     TNLLSNALRFTDEGHIILRSRTDGEQWLVEVEDSGCGIDPAKLAEIFQPFVQVSGKRG
                     GTGLGLTISSRLAQAMGGELSATSTPEVGSCFCLRLLLRIATAPVPKTVNQAVRLDGL
                     RLLLIEDNPLTQRITVEMLNSSGAQVVAVGNAAQALETLQNSEPFAVALVDFDLPDID
                     GITLARQLAQQYPSLVLIGFSAHVIDETLRQRTSSLFRGIIPKPVPREVLGQLLAHYL
                     QLQVNNDQPLDVSQLNEDAQLMGTEKIHEWLVLFTQHALPLLDDIDIARANQDSEKIK
                     RAAHQLKSSCSSLGMRIASQLCAQLEQQPLSAPLPHEEITRSVAALEAWLHKKDLNAI
                     "
     gene            5435..6463
                     /gene="torT"
                     /locus_tag="ACHKBL_003043"
     CDS             5435..6463
                     /gene="torT"
                     /locus_tag="ACHKBL_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system periplasmic protein TorT"
                     /protein_id="HFO5819885.1"
                     /translation="MRVLLLLLLSLFMLSAFSADNLLRWHDAQHYTVQASTPLKAKRA
                     WKLCALYPSLKDSYWLSLNYGMQEAARRYGVDLKVLEAGGYSQLATQQAQIDQCKQWG
                     AEAILLGSSTTSFPDLQKQVANLPVIELVNAIDAPQVKSRVGVPWFQMGYQPGRYLVQ
                     WAHGNPLNVLLMPGPDNAGGSKEMAEGFRAAIAGSPVRIVDIALGDNDIEIQRNLLQE
                     MLERHPEIDVVAGTAIAAEAAMGEGRNLKTPLTVVSFYLSHQVYRGLKRGRVIMAASD
                     QMVWQGELAVEQAIRQLQEQSVSDNVSPPILVLTPKNADREHIRRSLSPGGFRPVYFY
                     QHTSAAKK"
     gene            complement(6436..7128)
                     /gene="torR"
                     /locus_tag="ACHKBL_003044"
     CDS             complement(6436..7128)
                     /gene="torR"
                     /locus_tag="ACHKBL_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator TorR"
                     /protein_id="HFO5819886.1"
                     /translation="MPHHIVIVEDEPVTQARLQSYFTQEGYTVSVTASGAGLREIMQN
                     QPVDLILLDINLPDENGLMLTRALRERSTVGIILVTGRSDRIDRIVGLEMGADDYVTK
                     PLELRELVVRVKNLLWRIDLARQAQPHTQDNCYRFAGYCLNVSRHTLERDGEPIKLTR
                     AEYEMLVAFVTNPGEILSRERLLRMLSARRVENPDLRTVDVLIRRLRHKLSADLLVTQ
                     HGEGYFLAADVC"
     gene            7258..8430
                     /gene="torC"
                     /locus_tag="ACHKBL_003045"
     CDS             7258..8430
                     /gene="torC"
                     /locus_tag="ACHKBL_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pentaheme c-type cytochrome TorC"
                     /protein_id="HFO5819887.1"
                     /translation="MRKLWNALRRPSARWSVLALVAIGIVIGIALIVLPHVGIKVTST
                     TEFCVSCHSMQPVYEEYKQSVHFQNASGVRAECHDCHIPPDIPGMVKRKLEASNDIYQ
                     TFIAHSIDTPEKFEAKRAELAEREWARMKENNSATCRSCHNYDAMDHAKQHPEAARQM
                     KVAAKDNQSCIDCHKGIAHQLPDMSSGFRKQFDELRASANDSGDTLYSIDIKPIYAAK
                     GDKEASGSLLPASEVKVLKRDGDWLQIEITGWTESAGRQRVLTQFPGKRIFVASIRGD
                     VQQQVKTLEKTTVADTNTEWSKLQATAWMKKGDMVNDIKPIWAYADSLYNGTCNQCHG
                     APEISHFDANGWIGTLNGMIGFTSLDKREERTLLKYLQMNASDTAGKAHGDKKEEK"
     gene            8430..10976
                     /gene="torA"
                     /locus_tag="ACHKBL_003046"
     CDS             8430..10976
                     /gene="torA"
                     /locus_tag="ACHKBL_003046"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0050626 - trimethylamine-N-oxide reductase
                     (cytochrome c) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
                     /protein_id="HFO5819888.1"
                     /translation="MNNNDLFQASRRRFLAQLGGLTVAGMLGPSLLTPRRATAAQAAT
                     EAVISKEGILTGSHWGAIRATVKEGRFVAAKPFELDKYPSKMIAGLPDHVHNAARIRY
                     PMVRVDWLRKRHLSDTSQRGDNRFVRVSWDEALDMFYEELERVQKTHGPSALLTASGW
                     QSTGMFHNASGMLAKAIALHGNSVGTGGDYSTGAAQVILPRVVGSMEVYEQQTSWPLV
                     LQNSKTIVLWGSDLLKNQQANWWCPDHDVYEYYEQLKAKVAAGEIEVISIDPVVTSTH
                     EYLGREHVKHIAVNPQTDVPLQLALAHTLYSENLYDKNFLANYCVGFEQFLPYLLGEK
                     DGQPKDAAWAEKLTGINAETIRGLARQMAANRTQIIAGWCVQRMQHGEQWAWMIVVLA
                     AMLGQIGLPGGGFGFGWHYNGAGTPGRKGVILSGFSGSTSIPPVHDNSDYKGYSSTIP
                     IARFIDAILEPGKVINWNGKSVKLPPLKMCIFAGTNPFHRHQQINRIIEGWRKLETVI
                     AIDNQWTSTCRFADIVLPATTQFERNDLDQYGNHSNRGIIAMKQVVPPQFEARNDFDI
                     FRELCRRFNREEAFTEGLDEMGWLKRIWQEGVQQGKGRGVHLPAFDDFWNNKEYVEFD
                     HPQMFVRHQAFREDPDLEPLGTPSGLIEIYSKTIADMNYDDCQGHPMWFEKIERSHGG
                     PGSQKYPLHLQSVHPDFRLHSQLCESETLRQQYTVAGKEPVFINPQDASARGIRNGDV
                     VRVFNARGQVLAGAVVSDRYAPGVARIHEGAWYDPDKGGEPGALCKYGNPNVLTIDIG
                     TSQLAQATSAHTTLVEIEKYNGTVEQVTAFNGPVEMVAQCEYVPASQVKS"
     gene            10973..11572
                     /gene="torD"
                     /locus_tag="ACHKBL_003047"
     CDS             10973..11572
                     /gene="torD"
                     /locus_tag="ACHKBL_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051259 - protein complex oligomerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HFO5819889.1"
                     /translation="MTTLTAQQIACVYAWLAQLFSRELDDEQLTQIASAQMAEWFSLL
                     KSEPPLTAAVNELENRIATQTVRDDARLELAADFCGLFLMTDKQAALPYASAYKQDEQ
                     EIKRLLVEAGMETSGNFNEPADHLAIYLELLSHLHFSLGEGTVPARRIDNLRQKTLTA
                     LRQWLPEFAARCHQYDSFGFYAALSQLLLVLVECDHQNR"
     gene            complement(11827..12132)
                     /gene="cbpM"
                     /locus_tag="ACHKBL_003048"
     CDS             complement(11827..12132)
                     /gene="cbpM"
                     /locus_tag="ACHKBL_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HFO5819890.1"
                     /translation="MANVTVTFTITEFCLHTGISEEELNEIVGLGVVEPREIQETTWV
                     FDDHAAIVVQRAVRLRHELALDWPGIAVALTLMDDIAHLKQENRLLRQRLSRFVAHP"
     gene            complement(12132..13052)
                     /gene="cbpA"
                     /locus_tag="ACHKBL_003049"
     CDS             complement(12132..13052)
                     /gene="cbpA"
                     /locus_tag="ACHKBL_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="curved DNA-binding protein"
                     /protein_id="HFO5819891.1"
                     /translation="MELKDYYAIMGVKPTDDLKTIKTAYRRLARKYHPDVSKEPDAEA
                     RFKEVAEAWEVLSDEQRRAEYDQMWQHRNDPQFNRQFHHGDGQSFNAEDFDDIFSSIF
                     GQHARQSRQRPATRGHDIEIEVAVFLEETLTEHKRTISYNLPVYNAFGMIEQEIPKTL
                     NVKIPAGVGNGQRIRLKGQGTPGENGGPNGDLWLVIHIAPHPLFDIVGQDLEIVVPVS
                     PWEAALGTKVTVPTLKESILLTIPPGSQAGQRLRVKGKGLVSKKQTGDLYAVLKIVMP
                     PKPDENTAALWQQLADAQSSFDPRKDWGKA"
     gene            13205..13306
                     /locus_tag="ACHKBL_003050"
                     /pseudo
     CDS             13205..13306
                     /locus_tag="ACHKBL_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415521.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            13587..14828
                     /gene="agp"
                     /locus_tag="ACHKBL_003051"
     CDS             13587..14828
                     /gene="agp"
                     /locus_tag="ACHKBL_003051"
                     /EC_number="3.1.3.10"
                     /EC_number="3.1.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glucose-1-phosphatase/inositol
                     phosphatase"
                     /protein_id="HFO5819892.1"
                     /translation="MNKTLIAATVAGIVLLASNAQAQTVPEGYQLQQVLMMSRHNLRA
                     PLANNGSVLEQSTPNKWPEWDVPGGQLTTKGGVLEVYMGHYMREWLAEQGMVKSGECP
                     PPDTVYAYANSLQRTVATAQFFITGAFPGCDIPVHHQEKMGTMDPTFNPVITDDSAAF
                     SEQAVAAMEKELSKLQLTDSYKLLEQIVNYKDSPACKEKQQCSLVDGKNTFSAKYQQE
                     PGVSGPLKVGNSLVDAFTLQYYEGFPMDQVAWGEIKSDQQWKVLSKLKNGYQDSLFTS
                     PEVARNVAKPLVSYIDKALVTDRTSAPKITVLVGHDSNIASLLTALDFKPYQLHDQNE
                     RTPIGGKIVFQRWHDSKANRDLMKIEYVYQSAEQLRNADALTLQAPAQRVTLELSGCP
                     IDANGFCPMDKFDRVLNEAVK"
     gene            complement(14866..15093)
                     /gene="yccJ"
                     /locus_tag="ACHKBL_003052"
     CDS             complement(14866..15093)
                     /gene="yccJ"
                     /locus_tag="ACHKBL_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002898701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccJ family protein"
                     /protein_id="HFO5819893.1"
                     /translation="MPTQEAKAHHVGEWASLRNTSPEIAEAIFEVAGYDEKMAEKIWE
                     EGSDEVLVKAFAKTDKDSLFWGEQTIERKNV"
     gene            complement(15114..15692)
                     /gene="wrbA"
                     /locus_tag="ACHKBL_003053"
     CDS             complement(15114..15692)
                     /gene="wrbA"
                     /locus_tag="ACHKBL_003053"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000607019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003955 - NAD(P)H dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HFO5819894.1"
                     /translation="MINHILRHVETMARAVAEGASKVDGAEVVVKRVPETMPPQLFEK
                     AGGKTQTAPVATPQELADYDAIIFGTPTRFGNMSGQMRTFLDQTGGLWASGALYGKLA
                     SVFSSTGTGGGQEQTITSTWTTLAHHGMVIVPIGYAAQELFDVSQVRGGTPYGATTIA
                     GGDGSRQPSQEELSIARYQGEYVAGLAVKLNS"
BASE COUNT         3833 a         3960 c         4105 g         3800 t
ORIGIN      
        1 tttataacta ttaggtgcag agatattcgc ttaaatggat ttgttgctcg gtgaaaaagt
       61 aacttgcgtt ataatttatg gtgttgacta ttgagctatt ccaataaaac cagccaactt
      121 taaacaagca actcatatta agagagataa acatttgccg ctgttggtcc tcgcaggcca
      181 tttacgcggc aaaattccac gcgtaatcct ggtataagca cttctgcgtc gcggggagtg
      241 aatgcggaaa tatggacctg gacttcttta cgaccgtcgg aggggataat gaatcctttg
      301 ccgcttttgc gatcaaaggt tttgacaatt cctgtcattt tacgggacaa acaaattcct
      361 tactgaaaat actgcgctgc actatacggg gttaataaaa taaagccagc gatatttaag
      421 accgccggac ggctaaaata aaatttgctt aatctcaatt atcatgcgtt aatagctgcg
      481 tcggtttgaa agacagacag catacaaagt agtttactaa agcagttctc attatcaggc
      541 attatcccct tcttttgagt ctctctcctg aacactaagt agtttctgta ctaaagccct
      601 gtttgccgaa aggcccaaaa tgaaggaagt aaaatatgtc taataaaatg actggtttag
      661 taaaatggtt taacgcagat aaaggttttg gctttatcac tcctgatgat ggcagcaaag
      721 acgttttcgt ccatttcacc gccatccaga gcaatgaatt ccgcacgctg aacgaaaatc
      781 agaaagttga attttctatt gagcaggggc aacgtggtcc cgcggcagcg aacgttgtta
      841 cgctctaagg ttgccgttat tactccacat ctccatttcc gctgtccatg ttgtcatggt
      901 tcacagtacc gcacatcggc attcgatgtg acggagcgaa atcctttagg ggcaaagtgt
      961 attttttgta aatcgacgat gatcaccttt gataacgtcg cgctgcaaat acgcactgac
     1021 catgcgccgc tggatttcac aaaataatat caggctccct cgtggagcct tttttatatc
     1081 tgccttattt ttcttcaacg ctgtatgtat agtaagcgat aacctgttga ttattgaatc
     1141 tttcggggag atggcttata acatttctta cctgaccagg gtaccgggaa ccaacacctt
     1201 actggcgtgt tgctgtcttt caagaccaga agaggttaac agtgaatatt gaagagttaa
     1261 aaaaacaagc cgaaacggaa atcgctgact ttatcgcgca aaaaatcgcc gagctgaaca
     1321 agaatacagg gaaagaagtc tctgaaattc gcttcaccgc acgagaaaaa atgaccgggc
     1381 ttgaaagtta tgatgtcaaa atcaaaataa tgtgattttg tgaacatcac ccgtgcgagg
     1441 tgatgttccg cttgttgcta atttagtggc caatcattgg cgcttgtgga attaagcgtc
     1501 ggtacaattc ctccggcacc gggctttgcc atactcccgc atacattgcg taaccaatca
     1561 ccgcaaacat aatccccaga atcagtagcg tcattaacca gccagacaac gcaaaggctt
     1621 ttttatttgc cgcaggtttt tgcagtgaaa aggtcaatgt tgaggctacc ggacatgact
     1681 ctacgcaagt catacagccg gtacattcca ctgttcgtac ctgaattaat ttatcgaccg
     1741 ggatccgtga tgggcaattt tttgcgcatt tgccacagtc gatacaactt tcggcattgc
     1801 gacgaatctt aaacggcgat aatagcgaaa ccacgcccat cagcgcgcca tatgggcaaa
     1861 gataacgaca ccaggcatgg cgaataaaca ggctggcaat cagcaaaacg atcatgctga
     1921 ttaatgtcgc ggtccccata tgacgaaaga aattgagcat tttaacgtcc atcaccacgc
     1981 tgtagggcga caacataaaa tagtgaatcg cctgagcggg catcaataac gcaatataga
     2041 gaaaaaaact caacagcaaa tacttcacgc cgcgcagagg agtatccagc cagcggggaa
     2101 ggacaaattg ccgaccaaac agtttgttac cgagatcgcc gattaattca gaaagcgtac
     2161 caaccgggca taaccatgaa caaaaggcct tttttaataa tagactgatg acgataaaag
     2221 cgaccaataa cagcatcgcg gcggcgtgga cggacggtaa ctggcctgtt gcaaggctat
     2281 atttcagatt catcagaccg gcaatcggta gccagccttc gatacctccc ggtctggtga
     2341 caaatgtcgt actacctgcc gtttcgtaat agcgcaccca ataccagaac gtgatggcaa
     2401 tataaatatt cattgccaac agtaataatt gcgtcgcttt acgccaggtc gtggcattgc
     2461 gccagtcatt ccacggtaat ttgccgcccg tcgtgcctgg ccgccgctgc cagcgggttc
     2521 ttcttttctc tgtcatgatt ttgccagtcc gttaagttgc ataccaaatg ccactattct
     2581 agttgttctt aactggctga tattgattca aatcgcgttc aggtctttct tatgcaacca
     2641 tgcttccaga gcggcaacac tgcgtgtaat ttcttcgtgt ggaagggggg cagataatgg
     2701 ctgctgctcc agttgtgcgc atagctggct ggcgatacgc attcccaggc tcgagcaact
     2761 gctttttagc tgatgtgcgg cacgctttat tttctcgcta tcctgattgg cgcgggcaat
     2821 gtcgatgtca tcgagaagcg gcagggcatg ttgtgtaaat aataccagcc attcgtggat
     2881 cttctccgtc cccattaact gagcatcttc attgagttgc gatacatcca gcggttgatc
     2941 gttattgact tgcagttgga gatagtgcgc cagtaactga ccgagcactt cacgcggcac
     3001 cggtttaggg ataatcccgc gaaatagcga actggtacgc tggcgcaacg tttcgtcgat
     3061 gacatgggcg ctaaagccaa tcaaaaccag cgacggatat tgctgtgcca gttgccgggc
     3121 aagcgtaatg ccgtcgatat ccggcagatc aaaatccacc agtgcgacag caaatggttc
     3181 actattttgc agtgtctcta aagcctgcgc ggcatttcct acggcaacga cctgcgcccc
     3241 actgctgttc aacatctcaa cggtaatccg ctgggttagc gggttatctt caattaacag
     3301 caaacgtaaa ccgtccagac gcaccgcctg attgactgtt tttggcacgg gtgcagtggc
     3361 aatacgtaac agcaagcgta aacaaaaaca gcttccaacc tccggcgtgc tggtggcgct
     3421 cagttcgcca cccatcgcct gggccagacg gctactgata gtcagcccca gcccggtgcc
     3481 gccgcgtttg ccgctaacct gaacaaatgg ctggaagatt tcagccagtt tcgcgggatc
     3541 aataccgcag ccgctgtctt ccacttcgac cagccattgt tcgccatcag tgcgactacg
     3601 caggataatg tgcccttcgt cggtgaaacg cagggcgttg ctcaacaggt tggtaatgac
     3661 ctgacgaata cgctgcggat cgcccattaa cgcagtcggt acatcgtcgg caattgccgt
     3721 tgccaggcga atcgggcgac ctttcacccg tccgctcatt aattgcaggg tactttccag
     3781 caacgggcgc ggttcaaagg gttcatcgct gaccgaaaca ttcttgccgc ctgcttcgat
     3841 agccgaataa tcgagaatat cgttgaggat ggtcagcaac gattcgccag agtcggtaat
     3901 tgcccgcaaa tcatcacgct gagcgttaag tgcggggtta tctgccagta gctgtgcggt
     3961 accgagaata ccgtacagcg gtgtgcggat ctcatggctc atcgccgcca gaaacgctga
     4021 ttttgcctgg ttggcttttt ctgcttccgc ccgtgcctgt cggtgttcta tcaccagttc
     4081 ctgcaattca gctgtacgcg ctttgacctg cgccgccaac tgttcacggt ggcgattcag
     4141 tgcatgaaca ctgctgcgaa acgcatccat cagccgcccg atggtgtcca gttcccgcac
     4201 gccagcggtt tccgggaaag gggagtcgat atcaccatcc agcaagcgtt gtagcgcctg
     4261 cgtttgttct gcaagagggc gcgtgactga gcgataaacc acgcgccaga ggatcagaat
     4321 cagtgcgcaa agagaaacta ttcccagcaa taacaggctg tattgcccgc gtgcactggc
     4381 tttttccaga tgtgccagcc cgtgctgatt acgcagctca atggtgtcga ccagctgact
     4441 gacttcgcta ctaaactgcg cgaattgggc gatgttattt tgggcgagag tttgcaggtg
     4501 attgctgatt tcactgtcct gctgatacag cgccagcaaa tcgctatatt ggctaacggt
     4561 agttaacgtt gttgctacct gagtacgaac acccggatct tcaatgcgta tttgccgacg
     4621 ttgcagaatt ttcaccgcat tattgagctg cttttccagc gttggcgcat ttttctggat
     4681 ctgttccagc cccagattca tcaccatttg ctgcacccgc agggcgctaa ggcgcagttc
     4741 attcatctgg ttaacatact caagatcgat atcaatcagc cgatcgagtg cactttctgc
     4801 ggcctgacgc tgatgttgtt cgatcaaatc gtaaatcccg gcctgggtcg ctccggcgga
     4861 ggttgccgca ttattcgcct gaccttgcgc caggcgtgcg atttcatcgg cggcggcgac
     4921 tatctgctga ctgagttgct gttgttgctg gcgaagttgc aaacgctgcc ccaccagttc
     4981 cccttgctga cgtaacgaac gggagatctc ctgctcctgt tgttcaatag cggtggtgtc
     5041 aaaatcttgt tcccgtaacg cttgcagcaa cgcattaatc ttcaggcttt gtgcggtgag
     5101 cattcgcccc tgcgcctgcc acatcttttc gttatcggca ctggtcaggt tctgcgcggc
     5161 gaaaagttcc caggcgctgg cttcgctcaa ctggcgcgcc atattcatgg taggaatcaa
     5221 tgcctgagtg ttgtcttttt ccacctggct gataaagcgc aggttgtacc atcccaccag
     5281 ggtactggtc agggttaaca gcgccatcag ggcaaagccc atccagagtc ttcgggtcag
     5341 ggttaaattc acggtcggtg cactttgggt gaaaaaggtt gagtcgcaaa gcggaatgca
     5401 tctagcataa agccttatta ttgatgaggc tatcatgcgc gtactgctat tgttactact
     5461 ttcccttttc atgttatcgg cattttcggc tgataacctg ttgcgctggc atgatgcaca
     5521 gcattacacg gtgcaagcct ctacgccgct aaaagccaaa cgcgcatgga aactgtgcgc
     5581 gctttatccc agtttgaaag attcatactg gttatcgttg aactatggta tgcaggaggc
     5641 tgctcgccgc tacggtgtgg atttaaaagt gctggaggca ggcggctaca gccagttggc
     5701 tacccagcaa gcacaaatcg accagtgtaa acagtggggc gcagaggcca ttttgctcgg
     5761 tagtagcacg acgtcatttc ccgatctgca aaagcaggta gcaaatctgc cggtgatcga
     5821 actggtaaat gctattgatg ctccccaggt gaaaagccgc gttggtgtgc cctggtttca
     5881 gatgggctat caacctgggc gatatctggt gcaatgggcg cacggtaacc cactgaacgt
     5941 gctgttgatg cccggacccg ataacgccgg gggcagtaaa gagatggccg agggttttcg
     6001 cgcagctatt gctggaagcc cggtacgtat tgttgatatt gcgcttggcg ataacgatat
     6061 tgaaatccag cgtaacctgt tgcaggagat gctggagcgc catccagaaa tcgacgtcgt
     6121 tgccggaacg gccattgcgg cagaggcggc aatgggtgaa gggcgtaacc tgaaaacgcc
     6181 gcttaccgtg gtgtcgtttt atctttcaca tcaggtgtat cgcgggctga agcggggaag
     6241 agtgattatg gctgccagcg atcagatggt ctggcagggg gaactggcgg ttgagcaggc
     6301 catcaggcaa ttacaggagc aatcggtgtc tgataatgtc agcccaccga ttttagttct
     6361 gacgccgaaa aatgccgacc gcgaacatat ccgccgctcg ctgtcaccgg ggggatttcg
     6421 tccggtctat ttttatcagc acacatcagc ggctaagaaa taaccttcgc catgttgcgt
     6481 caccagtaaa tccgcgctga gtttatggcg taaacgacga attaacacat cgacggtgcg
     6541 caagtcaggg ttttccaccc gacgcgcaga gagcatacgc agcagacgtt cacggctaag
     6601 aatttcgccc ggattcgtca caaatgccac caacatttca tactctgcgc gggtcagttt
     6661 aatcggctcg ccatcccgct ccagcgtatg gcgcgacaca ttcaggcaat aaccggcaaa
     6721 gcgatagcag ttgtcctgtg tgtgcggttg agcttgtcgc gcgaggtcga ttcgccagag
     6781 cagatttttc acccgtacta ccagttcgcg cagttccagc ggtttggtga cgtaatcgtc
     6841 tgcgcccatt tccagcccaa caatacggtc aatccgatcg ctgcgtcccg taaccagaat
     6901 aatccccacc gttgagcgtt ctcgcagggc gcgggttaac atcaggccat tttcatcggg
     6961 caaattaata tccagcagaa ttaaatccac cggctgattc tgcataattt cccgcagccc
     7021 ggcaccgctc gccgtaacgg aaacggtata cccctcctga gtgaagtagg attgtaatcg
     7081 cgcctgggta accggctcat cttcaacaat aacaatgtga tgtggcatca gagggtttta
     7141 ctcattctgt tcatatctgt tcatattctg ccgtaagccg ttcatcctga ccagtgccgc
     7201 tgttcatatt tactcattaa gatcgcttca ctaaaccata attttacagg ggctattatg
     7261 cggaaactct ggaacgcgct acgccgaccc agtgctcgtt ggtcggtact ggcgctggtc
     7321 gctattggga ttgtgattgg cattgcgctg attgtattgc cacacgttgg gatcaaagtc
     7381 accagcacaa ccgaattttg tgtcagttgc cacagtatgc aaccggtgta tgaagaatat
     7441 aaacagtcgg tgcacttcca gaacgcctcc ggcgtgcgag ctgaatgcca tgactgtcat
     7501 atcccgccgg atattccagg catggtgaag cgcaaactgg aagcgagcaa cgacatttac
     7561 cagaccttta ttgcccactc cattgataca cctgaaaaat tcgaagccaa acgcgcggaa
     7621 cttgccgagc gtgaatgggc gcgaatgaaa gaaaacaact cggcaacctg tcgctcctgc
     7681 cataactacg atgcgatgga tcatgccaag cagcatcctg aagcggcgcg tcagatgaag
     7741 gtggcagcga aagataatca atcctgcatc gactgtcata aaggtattgc ccaccagtta
     7801 ccggatatga gtagtggctt ccgtaagcag ttcgatgagc tgcgcgccag tgctaatgat
     7861 agtggtgaca cgctgtactc cattgatatt aagccgattt atgcggcgaa aggcgataaa
     7921 gaagcgtctg gttctctgct gcctgcttcg gaagtgaaag tccttaaacg tgacggcgac
     7981 tggctgcaaa ttgaaatcac tggttggacg gaaagtgcag gacgtcagcg tgtactcacc
     8041 caattcccag gtaaacgcat ctttgttgcc tcgattcgtg gtgatgtgca gcagcaggtg
     8101 aaaacgctgg agaaaaccac cgttgccgac accaataccg agtggagcaa gttgcaggct
     8161 actgcgtgga tgaagaaagg cgacatggtg aacgatatca aaccgatctg ggcttatgcg
     8221 gattcgctgt acaacggcac ctgtaaccag tgccacggcg caccggaaat ctctcacttt
     8281 gacgctaacg gttggatcgg cacgctcaac ggcatgattg gctttaccag cctcgataaa
     8341 cgtgaagaac gcaccttgtt gaaatatctg caaatgaatg cgtctgacac cgcaggtaag
     8401 gctcacggcg ataagaagga agaaaaataa tgaacaataa cgatctcttt caggcatcac
     8461 gtcggcgttt tctggcacaa ctcggcggct taaccgtcgc cgggatgctg gggccgtcat
     8521 tgttaacgcc gcgccgtgca actgcggcgc aagcggcgac tgaggctgtc atctcgaaag
     8581 agggcattct taccgggtcg cactgggggg ctatccgcgc gacggtgaag gagggtcgct
     8641 tcgtggcagc aaaaccgttc gaactggata aatatccgtc gaaaatgatc gccggattgc
     8701 cggatcatgt acacaacgcg gcgcgtattc gttatccgat ggtacgcgtg gactggctac
     8761 gtaagcgcca tctgagcgac acctcccagc gcggtgataa ccgttttgtg cgcgtgagct
     8821 gggatgaagc cctcgacatg ttctatgaag aactggaacg cgtacagaaa actcacgggc
     8881 cgagtgcctt gctgaccgcc agtggttggc aatcgacggg gatgttccat aacgcttcgg
     8941 ggatgctggc gaaagcgatt gccttgcatg gtaatagcgt gggtacaggc ggagattact
     9001 ctaccggggc tgcgcaggtg atcctgccgc gcgtagtcgg ttcgatggaa gtgtatgaac
     9061 agcaaacctc ctggccgctg gtattgcaga acagcaaaac cattgtgctg tggggctccg
     9121 atttgctgaa aaaccagcaa gcgaactggt ggtgcccgga tcacgatgtt tatgaatatt
     9181 acgagcagct aaaagcgaaa gtcgccgccg gtgaaattga ggtcatcagc atcgatccgg
     9241 ttgtcacatc cacccatgag tatctggggc gcgagcatgt gaagcacatt gcggttaacc
     9301 cgcaaactga cgtgccgctg caactggctc tggcgcatac gctgtacagt gaaaacctgt
     9361 acgacaaaaa cttcctcgct aactactgtg taggttttga gcagttcctg ccgtatctgc
     9421 tgggtgagaa agacggtcag ccgaaagatg ccgcatgggc tgaaaaactg acaggtatta
     9481 atgccgaaac cattcgtggg ctggcgcggc agatggcggc gaacagaacg cagattattg
     9541 ctggctggtg cgtgcagcgt atgcagcacg gtgaacagtg ggcgtggatg attgtcgttc
     9601 tggcggcgat gttggggcaa attggcctgc caggtggagg ttttggtttt ggctggcact
     9661 ataacggcgc aggcacgccg gggcgtaaag gcgttattct gagtggtttc tccggctcta
     9721 cgtcgattcc gcctgttcac gacaacagcg actacaaagg ctacagcagc accattccga
     9781 ttgcccgttt tatcgatgcg atcctcgaac cggggaaagt gatcaactgg aacggtaaat
     9841 cggtaaaact accgccgctg aaaatgtgta tttttgccgg aactaacccg ttccatcgcc
     9901 atcagcagat caaccgcatt attgaaggct ggcgcaagct ggaaacggtt atcgccatag
     9961 ataaccagtg gacctcaacc tgccgctttg ccgatatcgt gctgcctgcg accacgcagt
    10021 ttgagcgtaa cgatctcgac cagtacggca accactccaa tcgtggcatt atcgccatga
    10081 aacaggtcgt gccgccgcag ttcgaggcgc gcaacgactt cgatattttc cgcgagctgt
    10141 gccgtcgctt taatcgcgaa gaagccttta ccgaagggct ggacgaaatg ggctggctga
    10201 aacgcatctg gcaggaaggt gtacaacagg gcaaaggacg cggcgttcat ctgccagcgt
    10261 ttgatgactt ctggaataat aaagagtacg tcgagtttga ccatccgcag atgtttgttc
    10321 gccaccaggc attccgcgaa gatccggatc tcgaaccgct gggcacgccg agtggcctga
    10381 ttgagatcta ctcgaaaacc atcgctgata tgaactacga cgattgtcag gggcatccga
    10441 tgtggtttga gaaaatcgaa cgctcccacg gtgggcccgg ctcgcagaag tatccgttgc
    10501 atctgcaatc tgtgcatccg gatttccgac ttcactcgca gttatgtgag tcggaaacgc
    10561 tgcgtcagca atatacggta gcgggcaaag agccagtgtt cattaacccg caggatgcca
    10621 gcgcgcgcgg tattcgtaac ggtgatgtgg tacgcgtgtt taacgctcgc ggtcaggtgt
    10681 tggcaggggc agtggtttct gaccgctatg cacccggcgt ggcgcgaatt catgaagggg
    10741 catggtacga tccagataaa ggcggcgagc ctggtgcgct gtgcaaatac ggtaacccca
    10801 acgtgttgac catcgacatc ggtacttcgc agttggcgca ggcgaccagt gcgcatacca
    10861 cgctggtgga aattgagaag tacaacggaa cagtggagca ggtgacggcg tttaacggcc
    10921 ctgttgagat ggtggcgcag tgcgaatatg ttcccgcgtc gcaggtgaaa tcatgaccac
    10981 gctgacagca caacagattg cctgtgttta cgcctggctg gcgcagttgt tctcccgtga
    11041 actggacgat gaacaactga cgcaaatcgc cagtgcgcag atggctgaat ggttttcgtt
    11101 gctgaaaagc gaaccgccgc tcactgcggc ggtgaatgag ctggaaaacc gtattgccac
    11161 tcagacagta cgtgacgatg ctcgtctgga actggccgct gacttttgcg gcctgttttt
    11221 gatgaccgac aaacaagctg cgctgccgta tgcatcggcc tacaaacagg acgagcaaga
    11281 gattaaacgc ttgttagttg aggcagggat ggaaaccagc ggcaatttca acgaaccggc
    11341 agaccatctg gcgatctatc tcgagttgtt gagtcatctg catttttcgc tgggagaggg
    11401 gaccgttcct gcgcgaagaa tcgacaatct gcggcaaaaa acactgacgg cgctgcgaca
    11461 atggttacca gagtttgctg cgcgttgcca tcagtacgac agtttcggtt tttacgcggc
    11521 actaagccag ttattgctgg tgttagtgga atgcgaccac caaaacagat aacgtcgttt
    11581 gttggcctga taagacgcgt ttagcgtcgc atcaggcatt agtgcgcagt tgccggatgc
    11641 ggcgagaacg ccttatccgg cctacgggaa cagtgctttt tgtgggcctg ataagatgcg
    11701 ttaagcgtcg catcaggcat tatggcgcag ttgccggatg cggcgagaac gccttatccg
    11761 gcctacggga acagtgcttt ttgtgggcct gataagacgc gttaagcgtc gcatcaggca
    11821 acaaactcac gggtgagcca caaaccggga aagccgctgg cgcagcaggc ggttttcctg
    11881 cttcaggtgc gcaatatcat ccattaacgt cagcgccacc gcgatccccg gccagtccag
    11941 agccagttca tgacgcaggc gtaccgcgcg ttgcaccaca atggcggcat ggtcgtcaaa
    12001 tacccaggtt gtctcctgga tctcccgcgg ttcaaccacc cccaaaccga caatttcatt
    12061 caactcctct tcagagatgc cggtatgcag gcaaaattcg gtaatagtaa aagtcaccgt
    12121 aacattagcc attatgcttt cccccaatct ttacgtggat caaaagacga ctgggcgtct
    12181 gccagttgct gccacagcgc ggcagtgttt tcatccggtt tcggcggcat cacgattttc
    12241 agtaccgcat acagatcgcc ggtctgtttt ttgctcacca gacctttgcc tttaacgcgc
    12301 aatcgttgcc cggcctggct gccaggcggg atagtcagca aaatgctttc tttcagtgtt
    12361 ggaacggtga ctttagtacc cagcgccgct tcccacgggc taaccggcac cactatttcc
    12421 agatcctggc cgacaatatc aaacagcgga tgtggcgcaa tgtgaatcac cagccacagg
    12481 tcgccatttg gaccgccgtt ttcgcccggc gtcccctggc ctttcaggcg gatgcgttga
    12541 ccattgccga cgcccgccgg gatcttcaca ttcagcgttt tcgggatttc ctgttcgatc
    12601 atgccaaagg cgttataaac cggcaggtta tagctgatgg tacgcttatg ctcagtaagc
    12661 gtttcttcga ggaaaaccgc cacttcgatt tcaatatcgt ggccgcgtgt ggcggggcgt
    12721 tgacggctct ggcgggcatg ctgaccgaaa attgacgaga agatatcgtc aaaatcttcg
    12781 gcgttaaaac tctgaccgtc gccatgatgg aactgacggt taaattgcgg atcgttgcga
    12841 tgttgccaca tctgatcata ctcagcgcga cgttgttcat cacttaacac ttcccaggct
    12901 tcggcgacct ctttgaagcg ggcttcggca tccggttctt tgctgacatc aggatggtat
    12961 ttgcgggcca gtcgacgata ggcggtcttg attgtcttga gatcgtccgt cggtttcacg
    13021 cccatgatgg cgtaataatc ctttaattcc atagcgttat ctcgcgtaaa tcaacacaaa
    13081 ttgaaggaac ccctgtaagg taactcctat aagtgtaggg taatcctcaa aatttcatat
    13141 gccaacacag aatatgttat tgaaatcatc gcggagggga ggtcgcctca aggtgggagg
    13201 aacatatttt atttacctcc ctcctgaact tttggctcat gcttacaagt taaacaataa
    13261 ggaaaggaat gcacaactcg actatttaaa tcgttatgca atttaatgat aaagacatgt
    13321 gctaaaaacc atttctatta gtctcacatt ctttttttat tggtaatggt aacgccgctg
    13381 attttcttta tagctactga tgtaatcatt agcggcgtaa acgaggccgg ggctaatcag
    13441 gcatagtttg cgtcaaaact tgcctgtttt taaaggagaa tatagaaaaa caggcgttca
    13501 acgagccatt ttgcgaacct gttcccggaa aaatgacata tttctgtcac actctttaat
    13561 tattgataac aaaagaggtg tgaggaatga acaaaacgct aatcgccgca actgtggcag
    13621 ggatagtttt actcgcttca aatgctcagg cacaaaccgt accggaaggc tatcagctac
    13681 agcaagtcct tatgatgagc cgtcataact tacgtgcgcc gctggcgaac aatggcagtg
    13741 tgctggagca gtcgacgccg aataaatggc ctgaatggga cgtccccggt gggcagctca
    13801 ccactaaagg gggcgtgctc gaagtgtata tgggccatta tatgcgtgaa tggctggcag
    13861 aacaggggat ggtgaaatca ggggaatgcc cgccaccgga caccgtttat gcctatgcca
    13921 atagtctgca acgcaccgtc gcgaccgcgc agttctttat tactggcgca ttccctgggt
    13981 gtgatattcc tgttcatcac caggaaaaaa tgggcactat ggaccccacc tttaacccag
    14041 tgatcaccga tgattccgcc gcattcagtg aacaggcggt ggcggcaatg gagaaagagc
    14101 tcagcaaact ccagcttacc gacagctaca agctactgga acaaatcgtt aactataaag
    14161 attctcctgc gtgtaaagag aagcaacaat gttcgcttgt agatggcaaa aataccttta
    14221 gcgcgaagta tcaacaagaa ccgggcgttt ccgggccgct gaaagtcggc aactcgctgg
    14281 tagatgcgtt tactttgcaa tactacgaag gttttccgat ggatcaggtg gcctggggag
    14341 aaatcaaatc tgaccagcag tggaaggtgt tgtcgaagct gaaaaacggt taccaggaca
    14401 gcctgtttac ctcaccggaa gtggcgcgca atgttgcgaa accgctggtc agttatatcg
    14461 acaaagccct ggtcaccgat cgcaccagtg caccgaaaat cacggtatta gttgggcacg
    14521 actccaacat tgcctctctg ttaacggcgc tggatttcaa accgtatcag ttgcatgacc
    14581 agaacgaacg cacgccgatt ggcggcaaaa tcgttttcca gcgttggcat gacagcaaag
    14641 ccaatcgcga tttgatgaaa attgaatatg tgtaccagag tgcggaacag ttacgtaatg
    14701 ccgatgcgtt aaccctgcag gcacctgcgc agcgcgtgac gctggaatta agcggttgcc
    14761 cgatagatgc caacggtttt tgcccaatgg ataagtttga tcgcgtattg aatgaagcgg
    14821 tgaaataaca gaaaactccc ccgcgtgaag cgggggagtc gctggttaaa cgtttttacg
    14881 ttcgatggtc tgttcgcccc aaaaaagcga atctttatcg gtcttagcaa aggctttgac
    14941 taacacttca tcgctacctt cttcccaaat cttttccgcc attttttcgt cgtacccggc
    15001 gacttcgaaa atggcctcgg ctatttccgg cgacgtattg cgcagagatg cccattcacc
    15061 gacgtgatga gctttcgctt cttgagttgg catgcgtatc ctcctgttga agattagctg
    15121 ttaagtttaa ctgccagacc tgcgacatat tccccttgat aacgagcaat agacagttct
    15181 tcctggctgg gctggcgtga accgtcaccg cctgcgatgg tggttgcgcc gtacggcgta
    15241 ccgccgcgaa cctgtgaaac gtcaaataat tcctgcgctg cgtagccaat ggggacaatc
    15301 accatgccgt gatgcgcaag ggtcgtccag gtggatgtga tggtttgttc ctgaccgccg
    15361 ccagtaccgg tggaactaaa gacgctcgcc agttttccgt atagtgcgcc ggaagcccac
    15421 aggccgcccg tctggtcgag gaaggtacgc atttgaccgg acatgttacc aaagcgggta
    15481 ggtgtaccaa aaataatggc gtcgtaatcg gccagttctt gcggggttgc aaccggtgca
    15541 gtttgcgttt taccgcctgc tttttcaaat aattgcggcg gcatggtttc cggtacacgc
    15601 ttaacgacaa cttctgcgcc atccactttg cttgcaccct cagcaactgc gcgtgccatc
    15661 gtttcaacat gtctaaggat atggtttatc attttgaa
//
LOCUS       DAZFLD010000116        15544 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.117, whole genome shotgun sequence.
ACCESSION   DAZFLD010000116 DAZFLD010000000
VERSION     DAZFLD010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15544
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.117"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            4..885
                     /gene="yieE"
                     /locus_tag="ACHKBL_003054"
     CDS             4..885
                     /gene="yieE"
                     /locus_tag="ACHKBL_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YeiE"
                     /protein_id="HFO5819895.1"
                     /translation="MHITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQ
                     LGVQLFDRVGKRLVVNEHGRLLYPRALALLEQAVEIEQLFREDNGAIRIYASSTIGNY
                     ILPAVIARYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLE
                     DELVVFAAPTSPLARGPVTLEQLAAAPWILRERGSGTREIVDYLLLSHLPKFEMAMEL
                     GNSEAIKHAVRHGLGISCLSRRVIEDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLS
                     NALRRFLDYCDPANVPR"
     gene            1090..2559
                     /gene="lysP"
                     /locus_tag="ACHKBL_003055"
     CDS             1090..2559
                     /gene="lysP"
                     /locus_tag="ACHKBL_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-specific permease"
                     /protein_id="HFO5819896.1"
                     /translation="MVSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATIS
                     QAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSGSFATYGQNYVEEGFGFALGWNY
                     WYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
                     LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSF
                     QGTELIGIAAGESEDPAKNIPRAVRQVFWRILLFYVFAILIISLIIPYTDPSLLRNDV
                     KDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYASTRMLYTLACDGKAPR
                     IFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAVS
                     HYRFRRGYVLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGV
                     AATYIGIPLFLIIWFGYKLIKGTHFVRYSEMKFPQNDKK"
     gene            2852..4831
                     /gene="cirA"
                     /locus_tag="ACHKBL_003056"
                     /pseudo
     CDS             2852..4831
                     /gene="cirA"
                     /locus_tag="ACHKBL_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311074.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
     gene            complement(4863..5699)
                     /gene="yeiG"
                     /locus_tag="ACHKBL_003057"
     CDS             complement(4863..5699)
                     /gene="yeiG"
                     /locus_tag="ACHKBL_003057"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HFO5819897.1"
                     /translation="MEMLEEHRCFEGWQQRWRHDSSTLNCPMTFSIFLPPPRDHTPPP
                     VLYWLSGLTCNDENFTTKAGAQRVAAELGIVLVMPDTSPRGEQVANDDGYDLGQGAGF
                     YLNATQPPWATHYRMYDYLRDELPALVQSQFNVSDRCAISGHSMGGHGALIMALKNPG
                     KYTSVSAFAPIVNPCSVPWGIKAFSSYLGEDKNAWLEWDSCALMYASNAQDAIPTLID
                     QGDNDQFLADQLQPAVLAEAARQKAWPMTLRIQPGYDHSYYFIASFIEDHLRFHAQYL
                     LK"
     gene            5957..6625
                     /gene="folE"
                     /locus_tag="ACHKBL_003058"
     CDS             5957..6625
                     /gene="folE"
                     /locus_tag="ACHKBL_003058"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HFO5819898.1"
                     /translation="MPSLSKEAALVHEALVARGLETPLRPPVHEMDNETRKSLIAGHM
                     TEIMQLLNLDLADDSLMETPHRIAKMYVDEIFSGLDYANFPKITLIENKMKVDEMVTV
                     RDITLTSTCEHHFVTIDGKATVAYIPKDSVIGLSKINRIVQFFAQRPQVQERLTQQIL
                     IALQTLLGTNNVAVSIDAVHYCVKARGIRDATSATTTTSLGGLFKSSQNTRHEFLRAV
                     RHHN"
     gene            6642..7799
                     /gene="yeiB"
                     /locus_tag="ACHKBL_003059"
     CDS             6642..7799
                     /gene="yeiB"
                     /locus_tag="ACHKBL_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 domain-containing protein YeiB"
                     /protein_id="HFO5819899.1"
                     /translation="MGRNVTLDFVRGVAILGILLLNISAFGLPKAAYLNPAWYGAITP
                     QDAWTWAFLDLIGQVKFLTLFALLFGAGLQMLLPRGRRWIQSRLTLLVLLGFIHGLLF
                     WDGDILLAYGLVGLICWRLVRDAPSVKSLFNTGVMLYLVGLGVLLLLGLISDSQTSRA
                     WTPDASAILYEKYWKLHGGVEAISNRADGVGNSLLALGAQYGWQLAGMMLIGAALMRS
                     GWLKGQFSLRHYRRTGFVLVAIGVTINLPAIALQWQLDWAYRWCAFLLQMPRELSAPF
                     QAIGYASLFYGFWPQLSRFKLVLAIACVGRMALTNYLLQTLICTTLFYHLGLFMHFDR
                     LELLAFVIPVWLANILFSVIWLRYFRQGPVEWLWRQLTLRAAGPARSKTSR"
     gene            7941..8981
                     /gene="galS"
                     /locus_tag="ACHKBL_003060"
     CDS             7941..8981
                     /gene="galS"
                     /locus_tag="ACHKBL_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalS"
                     /protein_id="HFO5819900.1"
                     /translation="MITIRDVARQAGVSVATVSRVLNNSTLVSADTREAVMKAVSELD
                     YRPNANAQALATQVSDTIGVVVMDVSDAFFGALVKAVDLVAQQHQKYVLIGNSYHEAE
                     KERHAIEVLIRQRCNALIVHSKALSDDELAQFMDNIPGMVLINRVVPGYAHRCVCLDN
                     LSGARMATRMLLNNGHQRIGYLSSSHGIEDDAMRKAGWMSALKEQDIIPPESWIGTGT
                     PDMPGGEAAMVELLGRNLQLTAVFAYNDNMAAGALTALKDNGIAIPLHLSIIGFDDIP
                     IARYTDPQLTTVRYPIASMAKLATELALQGAAGNIDPRASHCFMPTLVRRHSVATRQN
                     AAAITNSTNLAM"
     gene            9261..10259
                     /gene="mglB"
                     /locus_tag="ACHKBL_003061"
     CDS             9261..10259
                     /gene="mglB"
                     /locus_tag="ACHKBL_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HFO5819901.1"
                     /translation="MNKKVLTLFAVMASMLFGAAAHAADTRIGVTIYKYDDNFMSVVR
                     KAIEQDAKAAPDVQLLMNDSQNDQSKQNDQIDVLLAKGVKALAINLVDPAAAGTVIEK
                     ARGQNVPVVFFNKEPSRKALDSYDKAYYVGTDSKESGIIQGDLIAKHWAANQGWDLNK
                     DGQIQFVLLKGEPGHPDAEARTTYVIKELNDKGIKTEQLQLDTAMWDTAQAKDKMDAW
                     LSGPNANKIEVVIANNDAMAMGAVEALKAHNKSSIPVFGVDALPEALALVKSGALAGT
                     VLNDANNQAKATFDLAKNLADGKGAADGTNWKIDNKVVRVPYVGVDKDNLAEFSKK"
     gene            10320..11840
                     /gene="mglA"
                     /locus_tag="ACHKBL_003062"
     CDS             10320..11840
                     /gene="mglA"
                     /locus_tag="ACHKBL_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020883197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0043211 - ABC-type carbohydrate
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HFO5819902.1"
                     /translation="MVSSTTPSSGEYLLEMSGINKSFPGVKALDNVNLKVRPHSIHAL
                     MGENGAGKSTLLKCLFGIYQKDSGTILFQGKEIDFHSAKEALENGISMVHQELNLVLQ
                     RSVMDNMWLGRYPTKGMFVDQDKMYRETKAIFDELDIDIDPRARVGTLSVSQMQMIEI
                     AKAFSYNAKIVIMDEPTSSLTEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDE
                     VTVLRDGQWIATEPLAGLTMDKIIAMMVGRSLNQRFPDKENKPGEVILEVRNLTSLRQ
                     PSIRDVSFDLHKGEILGIAGLVGAKRTDIVETLFGIREKSAGTITLHGKKINNHNANE
                     AINHGFALVTEERRSTGIYAYLDIGFNSLISNIRNYKNKVGLLDNSRMKSDTQWVIDS
                     MRVKTPGHRTQIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDVGAKFEIYQLIA
                     ELAKKGKGIIIISSEMPELLGITDRILVMSNGLVSGIVDTKTTTQNEILRLASLHL"
     gene            11856..12866
                     /gene="mglC"
                     /locus_tag="ACHKBL_003063"
     CDS             11856..12866
                     /gene="mglC"
                     /locus_tag="ACHKBL_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HFO5819903.1"
                     /translation="MSALNKKSFLTYLKEGGIYVVLLVLLAIIIFQDPTFLSLLNLSN
                     ILTQSSVRIIIALGVAGLIVTQGTDLSAGRQVGLAAVVAATLLQSMDNANKVFPEMAT
                     MPIALVILIVCAIGAVIGLINGLIIAYLNVTPFITTLGTMIIVYGINSLYYDFVGASP
                     ISGFDSGFSTFAQGFVALGSFRLSYITFYALIAVAFVWVLWNKTRFGKNIFAIGGNPE
                     AAKVSGVNVGLNLLMIYALSGVFYAFGGMLEAGRIGSATNNLGFMYELDAIAACVVGG
                     VSFSGGVGTVIGVVTGVIIFTVINYGLTYIGVNPYWQYIIKGAIIIFAVALDSLKYAR
                     KK"
     gene            complement(12937..14172)
                     /gene="preA"
                     /locus_tag="ACHKBL_003064"
     CDS             complement(12937..14172)
                     /gene="preA"
                     /locus_tag="ACHKBL_003064"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017113 - dihydropyrimidine dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0006210 - thymine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
                     /protein_id="HFO5819904.1"
                     /translation="MLTKDLSITFCGVKFPNPFCLSSSPVGNCYEMCAKAYDTGWGGV
                     VFKTIGFFIANEVSPRFDHLVKEDTGFIGFKNMEQIAEHPLEDNLAALRRLKEDYPDK
                     VLIASIMGENEQQWEELARLVQEAGADMIECNFSCPQMTSHAMGSDVGQSPELVEKYC
                     RAVKRGSTLPMLAKMTPNIGDMCEVALAAKRGGADGIAAINTVKSITNIDLNQKIGMP
                     IVNGKSSISGYSGKAVKPIALRFIQQMRTHPELRDFPISGIGGIETWEDAAEFLLLGA
                     ATLQVTTGIMQYGYRIVEDMASGLSHYLADQGFDSLQEMVGLANNNIVPAEDLDRSYI
                     VYPHINLDKCVGCGRCYISCYDGGHQAMEWSEKTRTPHCNTEKCVGCLLCGHVCPVGC
                     IDLGEVKFKKGEKEHPVTL"
     gene            complement(14166..15404)
                     /gene="preT"
                     /locus_tag="ACHKBL_003065"
     CDS             complement(14166..15404)
                     /gene="preT"
                     /locus_tag="ACHKBL_003065"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreT"
                     /protein_id="HFO5819905.1"
                     /translation="MPQQNYLDELTPAFTPLLAIKEASRCLLCHDAPCSQACPAQTDP
                     GKFIRSIYFRNFKGAAETIRENNALGAVCARVCPTEKLCQSGCTRAGVDTPIDIGRLQ
                     RFVTDFEQQTGMEIYQPGTKTLGKVAIIGAGPAGLQASVTLTNQGYDVTIYEKEAQPG
                     GWLRNGIPQFRLPQSVLDAEIARIEKMGVTIKCNNEIGNTLTLEQLKAENRAVLVTVG
                     LSSGSGLPLFEHSDVEIAVDFLQRARQAQGDISIPQSALIIGGGDVAMDVASTLKVLG
                     CQAVTCVAREELDEFPASEKEFASARELGVSIIDGFTPVAVEGNKVTFKHVRLPGELT
                     MAADKIILAVGQHARLDAFAELEPQRNTIKTQNYQTRDPQVFAAGDIVEGDKTVVYAV
                     KTGKEAAEAIHHYLKGACSC"
BASE COUNT         3696 a         3835 c         4070 g         3943 t
ORIGIN      
        1 actatgcaca tcaccctccg gcagttggaa gtttttgcag aagtattgaa aagtggatca
       61 accacccagg cgtcggtgat gctggcgttg tcgcaatcag cagtgagcgc agccttgacc
      121 gatctggaag ggcagcttgg cgtgcaactg tttgatcgcg tggggaaaag actggttgtt
      181 aatgaacacg ggcggctgct ctatccgcgc gcgttggcac tgcttgaaca ggcggttgaa
      241 atcgaacaac tgtttcgcga agacaacggc gcgattcgta tctatgccag tagtactatc
      301 ggtaactaca ttctgcctgc agtcattgcc cgttatcgcc atgattatcc gcagttgccg
      361 attgaactta gcgttgggaa cagccaggac gtgatgcaag cggtgctgga tttccgcgtt
      421 gatattggct ttattgaagg accgtgccac agcactgaaa tcatttctga accgtggctg
      481 gaagacgagc tggtggtttt cgccgcgccg acttcgccat tggctcgtgg tccggtcacc
      541 ttagaacagc tggccgctgc gccgtggatc ctgcgtgaac gcggctccgg cacgcgggag
      601 attgtcgatt atctgttgct gtcacattta ccgaagtttg agatggcgat ggaattaggt
      661 aactccgagg caatcaaaca tgcggtgcgt catgggttgg gaattagttg cctgtcgcga
      721 cgggtgattg aagatcaatt gcaggcaggc acattaagtg aagttgcggt ccctctgccg
      781 cgcctgatgc gtacgttgtg gcgtatacat catcggcaaa aacacctttc caacgcgcta
      841 cggcgctttc tggactattg cgatcccgcg aatgtaccgc gttaagttgc tgtacaagaa
      901 catgctggtg ctgtgtcgat ttcgtgacgc agcgccctca gcatgcattc gccagaaaag
      961 agattggctg ctttacttat aatccctggg cgatcatgaa ggtgtcttat aaccgtgtat
     1021 ttctgccgga aggattgcca atcgtctgct acaatcgcgc ctcattttta agatggatag
     1081 catttttgta tggtttccga aactaaaacc acagaagcgc cgggcttacg ccgtgaatta
     1141 aaggcgcgtc acctgacgat gattgccatt ggcggttcca tcggtacagg tctttttgtt
     1201 gcctctggcg caacgatttc tcaggcaggt ccgggcgggg cattgctctc gtatatgttg
     1261 attggcctga tggtttactt cctgatgacc agtctcggtg aactggctgc atatatgccg
     1321 gtttccggtt cgtttgccac ttacggtcag aactatgttg aagaaggctt tggcttcgcg
     1381 ctgggctgga actactggta caactgggcg gtgactatcg ccgttgacct ggttgcagct
     1441 cagctggtca tgagctggtg gttcccggat acaccgggct ggatctggag tgcgctgttc
     1501 ctcggcgtta tcttcctgct gaactacatc tcagttcgtg gctttggtga agcggaatac
     1561 tggttctcac tgatcaaagt cacgacagtt attgtcttta tcatcgttgg cgtgctgatg
     1621 attatcggta tcttcaaagg cgcgcagcct gcgggctgga gcaactggac aatcggcgaa
     1681 gcgccgtttg ctggtggttt tgcggcgatg atcggcgtag ctatgattgt cggcttctct
     1741 ttccagggaa ccgagctgat cggtattgct gcaggcgagt ccgaagatcc ggcgaaaaac
     1801 attccacgcg cggtacgtca ggtgttctgg cgaatcctgt tgttctatgt gttcgcgatc
     1861 ctgattatca gcctgattat tccgtacacc gatccgagcc tgctgcgtaa cgatgttaaa
     1921 gacatcagcg tcagtccgtt caccctggtg ttccagcacg cgggtctgct ctctgctgca
     1981 gcggtgatga acgcagttat tctgacggcg gtgctgtcag cgggtaactc cggtatgtat
     2041 gcgtctactc gtatgctgta caccctggcg tgtgacggta aagcgccgcg cattttcgct
     2101 aaactgtcgc gtggtggcgt gccgcgtaat gcgctgtatg cgacgacggt gattgccggt
     2161 ctgtgcttcc tgacctccat gtttggcaac cagacggtat acctgtggct gctgaacacc
     2221 tccgggatga ccggttttat cgcctggctg gggattgccg ttagccacta tcgcttccgt
     2281 cgcggttacg tattgcaggg acacgacatt aacgatctgc cgtaccgttc aggtttcttc
     2341 ccactggggc cgatcttcgc attcattctg tgtctgatta tcactttggg ccagaactat
     2401 gaagcgttcc tgaaagatac cattgactgg ggcggcgtag cggcaacgta tattggtatc
     2461 ccgctgttcc tgattatttg gttcggctac aagctgatta aaggaactca ctttgtacgc
     2521 tacagcgaaa tgaagttccc gcagaacgat aagaaataag ttttctccct tccttgctaa
     2581 gccctctcaa ccgagagggc tttttcaatt ccatttccct gacaaatcat gcggatataa
     2641 aatttaacat ttggattgat aattgttatc gtttgcatta tcgttacgcc gcaatcaaaa
     2701 aaggctgaca aatcagaggc tggtctggct ttctgggatg atcacctgca tacaaataag
     2761 tccaccgcga tgctgccgta cgcaagggga cgtgaagaag atgtgagcga taacccattt
     2821 tattttcgta gttacctcat ggagatatgg aatgtttagg ttgaaccctt tcgtacgggt
     2881 cgggctgtgt ttgtccgcta tttcttgtgc atggcctgtg ttagcggtcg atgatgatgg
     2941 cgaaacgatg gttgtcactg catcttccgt tgagcaaaac cttaaagatg cacctgccag
     3001 tatcagcgtc attacctagg aagacctgca gcgaaaaccg gtacagaatc tgaaggatgt
     3061 cctcaaagaa gtgcctggcg tacaactgac gaacgaaggg gataaccgta agggcgttag
     3121 tattcgtggt ctggacagca gctacaccct gattctcgtc gacggtaaac gcgttaactc
     3181 ccgcaatgcc gtcttccgcc acaatgattt cgatctgaac tggatcccgg tcgattccat
     3241 cgaacgtatt gaagtggtcc gtggcccgat gtcgtcgctg tacggttccg atgcgctcgg
     3301 cggtgtagtg aatatcatca ccaaaaaaat cggtcagaaa tggtcgggca ccgttaccgt
     3361 cgataccacc gttcaggaac atcgcgatcg cggtgatacc tataacggtc aattctttac
     3421 cagcggacca ttaattgacg gcgtgctggg aatgaaagct tacggcagcc tggcaaaacg
     3481 tgaaaaggat gacccgcaaa actcaacgac caccgatacc ggagaaacgc cgcgtattga
     3541 aggattctcc agccgcgacg gcaatgtcga atttgcctgg acaccgaatc aaaatcacga
     3601 ttttactgcc ggatacggtt tcgaccgtca ggatcgtgat tccgactcgc tggacaaaaa
     3661 ccgcctggaa cgccagaact actccgtcag ccataatggg cgttgggatt acggcaccag
     3721 cgaactgaaa tactacggtg agaaagtcga gaacaaaaac cctggcaaca gcagcccgat
     3781 aacttccgaa agcaatacgg tcgacggcaa atacacgttg ccgctgacgg cgattaatca
     3841 gtttctcacg gttggcggtg aatggcgtca cgacaaactt agcgatgcgg tgaacctgac
     3901 tgggggaacc agctccaaaa catctgccag ccagtacgcg ctatttgttg aagatgaatg
     3961 gcggatcttc gagccgctgg cgctgacgac cggcgtacgt atggacgatc atgaaaccta
     4021 cggtgaacac tggagtccgc gtgcctacct ggtttataac gccaccgaca ccgtaacggt
     4081 gaaagggggc tgggcgacgg catttaaagc accttctctg ttgcaactta gccctgactg
     4141 gacgagcaat tcctgccgtg gcgcatgtaa gattgtgggt agcccggatc tgaaaccaga
     4201 aaccagcgaa agttgggagc tggggcttta ctacatgggc gaagaaggct ggctggaagg
     4261 ggttgaatcc agcgttaccg ttttccgtaa cgatgtgaaa gatcgtatca gcatcagccg
     4321 tacgtctgac gtcaatgctg caccaggcta ccaaaacttt gttggtttta cagcgaacgg
     4381 tgtgccggta tttagctact acaacgttaa caaagcacgt attcagggcg tggaaaccga
     4441 gctgaaaatt ccgttcaacg atgaatggaa actgtcactc aactacacct acaacgatgg
     4501 tcgtgatgtc agcaacggcg aaaataaacc tttatccgat ctgccgttcc ataccgctaa
     4561 cggtacgctg gactggaaac cgctggcgct ggaagactgg tcattctatg tttccggaca
     4621 ctataccggg cagaaacgcg ccgacagcgc gacggctaaa acaccgggcg gttataccat
     4681 ctggaatacc ggtgcggcct ggcaggtgac taaagacgtc aaactgcgcg caggcgtgct
     4741 gaaccttggc gacaaggatc tcagtcgtga cgactacagc tataacgaag acggacgtcg
     4801 ttactttatg gcagtggatt atcgcttctg atgagaagat gcccggcgaa ccgggcggac
     4861 tttcacttca gtaaatactg cgcatggaag cgcaggtgat cctctataaa agaggcgatg
     4921 aagtagtaac tgtgatcata tcccggctga atacgcagcg tcatcggcca cgctttctgg
     4981 cgggcggctt ctgccagcac tgcgggttgc agctggtcgg caagaaactg atcattatca
     5041 ccctgatcga taagcgtcgg gatcgcatcc tgcgcgttac tggcatacat cagcgcacaa
     5101 ctgtcccatt ccagccatgc atttttgtcc tcacctaaat agctgctaaa cgctttaatg
     5161 ccccagggta cgctgcatgg attcacaatc ggcgcaaagg cggaaacgct ggtgtatttg
     5221 cccgggtttt tcagcgccat aatcagcgca ccgtgaccac ccattgaatg accgctaata
     5281 gcgcagcggt cgctgacatt aaattgcgac tgaaccagcg ccggtaattc atcgcgcaga
     5341 taatcataca tccggtaatg cgtcgcccac ggcggttgcg tggcattaag ataaaagcct
     5401 gcgccctggc ctaaatcgta gccatcgtcg ttggcaacct gttcgccgcg cgggctggtg
     5461 tctggcatca ccagtacaat ccccagttct gccgccacgc gctgggcacc cgccttggtg
     5521 gtgaagttct cgtcattgca ggttaatccg gaaagccagt acagcactgg tggcggggtg
     5581 tgatcacgag gtggtgggag aaagatactg aacgtcatcg gacagtttaa ggtactggag
     5641 tcgtgtcgcc agcgttgctg ccagccttca aaacagcggt gctcttcgag catttccatg
     5701 cgtggctcct tgttgtgttg tttgcaattt cttcatcata ccgtttattc aatgtgctgt
     5761 gagtaacttt cacttccgta tttgcataac gatgttttaa catctgctga tgaaaggcag
     5821 cggcaattac aataattatc gctgtgaata ctggattatg tgcgccgcct cacgcacaat
     5881 aatcaggctg taaatcagct taataacttt gcccccacgc agggcggagg cgtcacacct
     5941 gcaggagaaa tcataaatgc catcactcag taaagaagcg gccctggttc atgaagcgtt
     6001 agttgcgcga ggactggaaa caccgctgcg cccgcccgtg catgaaatgg ataacgaaac
     6061 gcgcaaaagc cttattgctg gtcatatgac cgaaatcatg cagctgctga atctcgacct
     6121 ggctgatgac agtttgatgg aaacgccgca tcgcatcgct aaaatgtatg tcgatgaaat
     6181 tttctccggt ctggattacg ccaatttccc gaaaatcacc ctcattgaaa acaaaatgaa
     6241 ggtcgatgaa atggtcaccg tgcgcgatat caccctgacc agcacctgtg aacaccattt
     6301 tgttaccatc gatggcaaag cgacggtggc ttatatcccg aaagattcgg tgatcggtct
     6361 gtcaaaaatt aaccgcatcg tgcagttctt tgcccagcgc ccacaggtac aggaacgtct
     6421 gacccagcaa attctcattg cgctacaaac gctgctgggc accaataacg tggctgtctc
     6481 tatcgacgcg gtgcattact gcgtgaaagc gcgtggcatc cgcgacgcaa ccagtgccac
     6541 aacaacgacc tctcttggtg gattgttcaa atccagtcag aatacgcgcc acgaatttct
     6601 gcgcgctgtg cgtcatcaca actgattaaa aggcaggaac catggggcgc aacgtcacgc
     6661 tcgattttgt tcgcggcgtc gccattctgg ggatcctgct attaaacatc agcgcctttg
     6721 ggctaccaaa ggcggcttat cttaatcccg cctggtacgg cgctattacg ccgcaggatg
     6781 catggacctg ggcatttctc gatctcatcg gccaggtgaa attcctcacg ctttttgcgc
     6841 tgctgtttgg tgctggcctg caaatgttgc tgccccgtgg cagacgctgg atccagtcgc
     6901 ggttaacgct gttagtcttg ctgggcttta ttcacggttt attgttctgg gacggcgata
     6961 ttctgctggc ttacgggctg gtgggcttaa tctgctggcg gctggtgcgc gatgcgccat
     7021 cggtaaaaag cctgtttaat accggcgtca tgctttatct ggtggggctt ggcgttttgc
     7081 tgttattggg gttgatttcc gatagccaga ccagccgcgc ctggacacct gatgcatcgg
     7141 ctattttgta tgaaaagtac tggaaacttc acggtggtgt tgaagcgatc agtaatcgtg
     7201 ccgatggtgt tggcaacagt ttactggcac tgggcgcaca gtatggctgg caactggcag
     7261 ggatgatgct cattggtgcg gcgctgatgc gcagcggctg gctgaaaggg cagttcagct
     7321 tacgtcacta tcgtcgtact ggttttgtgc tggtggcgat tggggtgacc attaaccttc
     7381 ctgccatcgc cctgcaatgg cagctggact gggcgtatcg ctggtgtgcc tttttacttc
     7441 agatgccgcg ggaactgagt gcgccgtttc aggcgattgg ctatgcgtcg ctgttttatg
     7501 gcttctggcc gcaattgagc cgctttaagc tggtgcttgc gatcgcctgc gtcggacgga
     7561 tggcgctgac caactatcta ttgcaaacgc tgatttgtac cacgcttttt taccacctcg
     7621 gtttgtttat gcattttgac cgcctggagc tgctggcgtt tgttattccg gtatggctgg
     7681 cgaatatcct cttctctgtt atctggctgc gttacttccg ccaggggccg gtggaatggc
     7741 tctggcgtca gttaactttg cgtgcagctg ggccggcaag atctaaaaca tcaagataac
     7801 gatctggatc acattcgtta acaaaacggc tgtaaccgtt tccattgctg tgactcgatt
     7861 cacgaagtcc tgtattcagt gctgacaaaa tagccgccag caagcagtca tttactgcaa
     7921 tctcataaca ggtagtgaat atgatcacca ttcgtgatgt agcgcgtcag gctggcgtct
     7981 ctgtggcaac ggtttcccgg gtgctcaata acagcacgct ggtcagtgcc gacacgcgtg
     8041 aagcagtaat gaaagccgtg agtgagctgg attatcggcc aaacgccaat gcccaggcgc
     8101 tggcaactca ggttagcgat accattggcg tggtggtgat ggacgtttct gatgcgtttt
     8161 ttggtgcgct ggtaaaagcg gtggatttgg tcgcccagca gcatcagaaa tacgtgctaa
     8221 taggcaatag ctatcatgaa gcggaaaaag agcgtcacgc cattgaggtg ttaattcgcc
     8281 agcggtgtaa tgcgttgatt gttcactcaa aagcattgag tgatgatgaa ctggcacaat
     8341 ttatggataa cattcctggt atggtgttaa tcaaccgcgt tgtgccgggg tacgcccatc
     8401 gttgcgtttg cctggataat ctcagcggtg cccgaatggc gacgcgcatg ttgctgaata
     8461 acggtcatca acgtattggt tatctttctt ccagtcacgg cattgaagat gacgccatgc
     8521 gtaaagcagg ctggatgagt gcgttgaaag agcaggatat tattccgccg gaaagctgga
     8581 ttggcacagg tacgccggac atgccgggcg gtgaggcggc gatggttgaa ctgctggggc
     8641 gcaatctaca acttaccgct gtatttgctt ataacgacaa tatggccgct ggcgcactga
     8701 cagcattaaa agataatggc attgcgattc cgttacatct ctccatcatc ggtttcgatg
     8761 atattcccat cgcccgttac accgacccgc aattaacgac tgtgcgttat cccattgctt
     8821 caatggctaa attagccacc gaactggcct tgcagggggc agcaggcaat attgatccgc
     8881 gtgccagcca ctgttttatg ccgacgttag tgcgtcgcca ttctgtcgca acgcgccaga
     8941 atgcggcggc gatcactaac tcaacaaatc tggcgatgta accgctttca atctgtgagt
     9001 gatttcacag tatcttaaca atgtgatagc tatgattgca ccgttttaac gttgtaaccc
     9061 gtatgtaaca gtgaataatc actttcaccg aggtaatagc gtcataacaa caattaaagc
     9121 cgttttctgg agcgttaccg ggcatggaag aacgaatttt aaaaagtgag cttcggcgtt
     9181 cagtaacact tcattaactc tactgccccg ccgagcattt atctcaagca ctaccctgca
     9241 taagaaaaac cggagatacc atgaataaga aggtgttaac cctgtttgct gtgatggcca
     9301 gcatgttatt cggtgccgct gcacacgctg ctgatactcg cattggtgta acaatctata
     9361 agtacgacga taactttatg tctgtggtgc gcaaggctat tgagcaagat gcgaaagccg
     9421 cgccagatgt tcagctgctg atgaatgatt ctcagaatga ccagtccaag cagaacgatc
     9481 agatcgacgt attgctggcg aaaggggtga aggcactggc aatcaacctg gttgacccgg
     9541 cagctgcggg tacggtgatt gagaaagcgc gtgggcaaaa cgtgccggtg gttttcttca
     9601 acaaagaacc gtctcgtaag gcgctggata gctacgacaa agcctactac gttggcactg
     9661 actccaaaga gtccggcatt attcagggcg atttgattgc taaacactgg gcggcgaatc
     9721 agggttggga tctgaacaaa gacggtcaga ttcagttcgt actgctgaaa ggtgagccgg
     9781 gccatccgga tgcagaagca cgtaccactt acgtgattaa agaattgaac gacaaaggca
     9841 tcaaaactga acagttacag ttagataccg ctatgtggga taccgctcag gcgaaagata
     9901 agatggacgc ctggctgtct ggcccgaacg ccaacaaaat cgaagtggtt atcgccaaca
     9961 acgatgcgat ggcaatgggc gcggtagaag cactgaaagc acacaacaag tccagcattc
    10021 cggtgtttgg cgtcgatgcg ctgccagaag cgctggcgct ggtgaaatcc ggtgcactgg
    10081 cgggcaccgt actgaacgat gctaacaacc aggcgaaagc gacctttgat ctggcgaaaa
    10141 acctggccga tggcaaaggt gcggctgatg gcaccaactg gaaaatcgac aacaaagtgg
    10201 tccgcgtacc ttatgttggc gtagataaag acaacctggc tgaattcagc aagaaataag
    10261 actgattggg cgcagtcgtt tactgcgccc ttattgtatg gccaacaagg taaaattata
    10321 tggtcagctc aacgactccg tcttccgggg aatacttgtt ggaaatgagc ggtatcaaca
    10381 agtcttttcc aggtgttaag gcacttgata acgttaattt aaaagtccgg ccacattcta
    10441 tccatgcatt aatgggggaa aacggcgcag gaaaatcaac attattaaaa tgcctgtttg
    10501 gtatttatca aaaagactcc ggcaccattt tattccaggg taaagagatc gatttccatt
    10561 ctgccaaaga agcgctggaa aatggtattt cgatggtaca ccaggagtta aacctggtat
    10621 tacaacgttc ggtgatggac aacatgtggc tggggcgata tcccaccaaa ggcatgtttg
    10681 tcgatcagga caaaatgtac cgcgaaacca aagcgatttt tgatgaactg gatattgata
    10741 tcgatccgcg tgcgcgcgtc ggcacattat ccgtttcgca aatgcagatg atcgaaatcg
    10801 ccaaagcgtt ttcctataac gcgaaaattg tgattatgga tgaaccgact tcttcgttaa
    10861 ccgaaaaaga ggtcaatcat ctgttcacta ttattcgtaa attaaaagag cgcggctgcg
    10921 gtattgttta tatctcacat aaaatggaag aaatcttcca gttatgtgat gaagttaccg
    10981 tattgcgcga cggtcagtgg atcgccactg aaccgctggc aggactgacg atggacaaga
    11041 tcatcgccat gatggttggg cgttctctta accagcgttt ccctgacaaa gaaaacaagc
    11101 cgggcgaagt gattcttgaa gtgcgtaacc tgacgtcgct gcgccagccg tcgattcgcg
    11161 atgtctcgtt tgatctgcat aaaggggaga ttctcggtat agccggtctg gtgggagcga
    11221 aacgtaccga tattgttgag acgttatttg gtattcgcga gaaatcggct ggcaccatta
    11281 cgttgcacgg caaaaagatc aataaccata atgccaacga agccataaac cacggatttg
    11341 cactggtaac tgaagagcgc cgctcaacgg gaatttatgc ctatctggat atcggtttta
    11401 actcgttaat ttccaatatt cgcaactaca aaaataaagt tggtttactg gataactcgc
    11461 ggatgaaaag cgatacccag tgggtgattg attcgatgcg ggtaaaaaca ccggggcatc
    11521 ggacgcaaat tggttcgctc tccggcggta atcagcaaaa ggtgattatt ggtcgctggc
    11581 tactaacgca accagaaata ttaatgctcg atgaaccgac gcgcggtatt gatgtcggag
    11641 cgaagtttga gatctatcag ttaattgccg aactggcgaa gaaaggcaaa gggattatta
    11701 ttatctcctc tgaaatgcct gagttgttag ggataacaga ccgtattctg gtcatgagca
    11761 atggtctcgt ttccggaatt gtcgatacaa aaacaacaac gcaaaacgaa attctgcgtc
    11821 ttgcgtcttt gcacctttaa gattaggggc ttcccatgag tgcgttaaat aagaaaagtt
    11881 ttcttactta cctgaaagag ggcggtattt acgtcgttct tttagttttg ctggcaatta
    11941 ttattttcca ggacccaaca tttttaagtc tgttgaactt aagtaatatt ctcacccagt
    12001 catcggtgcg tattattatc gcgctcggtg tggcagggtt aattgtcacc caggggaccg
    12061 acctttctgc aggtcgccag gtggggctgg cggcagtagt agcggcgacg ttattgcagt
    12121 ccatggataa cgccaacaaa gtgttcccgg aaatggcgac gatgccgatt gcgctggtta
    12181 ttctgattgt ctgtgccatt ggtgcggtga tcggtttgat caacggtctg attatcgcct
    12241 atctcaacgt gacgccgttc attaccacgc tcggcacgat gatcatcgtc tatggcatca
    12301 actcgctcta ttacgacttt gtcggggcgt cgccgatttc tggtttcgac agtggcttct
    12361 ctacctttgc tcagggcttt gtcgcgctgg ggagtttccg tctctcttac atcaccttct
    12421 acgcgttgat tgcggtggcg ttcgtctggg tgttgtggaa caaaacccgc ttcggtaaga
    12481 acatttttgc cattggcggt aacccggaag cggcgaaagt atctggtgtc aacgtcggcc
    12541 tgaacctgct gatgatctac gcgttgtctg gcgtgttcta tgccttcggc gggatgttag
    12601 aagccggacg tatcggctct gccaccaaca acctcggctt tatgtatgag ttggatgcta
    12661 tcgcggcatg tgtggtgggt ggtgtatcgt tcagcggtgg tgtagggacg gtgattggcg
    12721 tggtgaccgg ggtgattatt tttaccgtca tcaactatgg cctgacgtac atcggcgtaa
    12781 acccatactg gcagtacatc atcaaagggg cgattattat cttcgccgtg gcgctggatt
    12841 cactgaaata cgcgcgtaag aaatgatgtg aatgtgggta ttgccggatg cgccgcatcc
    12901 ggcaatcggt gccaaatgcc ggatgcgtct ctggttttac aacgttaccg ggtgttcttt
    12961 ctctcccttc ttaaacttca cttctccgag atcaatacaa cccaccgggc agacgtgacc
    13021 acaaagcagg caaccaacac atttctcggt attacaatgc ggtgtacggg ttttctcgct
    13081 ccattccatc gcctggtgac cgccgtcgta acaggaaata taacagcgtc cacagccaac
    13141 acatttatcg agattgatat ggggatagac aatataactg cggtctaaat cttccgccgg
    13201 gacaatattg ttattcgcca gacctaccat ctcctgcaac gaatcaaacc cttgatcggc
    13261 gagataatgc gacaacccgc ttgccatatc ttccactatc cgatacccgt actgcataat
    13321 gccggtggtc acctgcaacg tcgctgcacc gagcaataaa aactccgccg catcttccca
    13381 ggtttcaatg ccgccgatac cgctgatcgg gaaatcgcgc agttctggat gggtacgcat
    13441 ttgctgaatg aagcgcaggg cgatcggttt taccgcttta ccggaatatc cggaaatact
    13501 cgatttaccg ttaacgatcg gcataccgat tttctgatta agatcgatat tggtgatgga
    13561 tttaacggtg ttaatcgccg caatgccatc tgcgccgccg cgcttcgccg ccagcgccac
    13621 ttcgcacata tcaccgatat tcggcgtcat cttcgccagc attggcagcg tggaaccacg
    13681 ttttactgcc cgacaatatt tttctaccag ctccgggctt tgcccgacat cgctacccat
    13741 cgcatgagaa gtcatttgcg gacaggagaa gttacactcg atcatatccg cgccagcttc
    13801 ttgcaccaga cgcgccagct cttcccattg ctgctcattt tcgcccatga ttgaagcgat
    13861 caatactttg tccgggtaat cttctttcag ccgacgcagt gcggccagat tatcttccaa
    13921 cggatgttca gcaatctgct ccatattttt gaagccgata aaaccggtat cttctttcac
    13981 cagatgatca aaacgcggcg agacttcgtt ggcgataaaa aaaccgatcg ttttaaacac
    14041 cacgccaccc cagcctgtgt cgtaggcttt ggcacacatc tcatagcagt tgcctacagg
    14101 cgaagaagag aggcagaacg ggttgggaaa cttcacacca caaaaggtaa tcgaaagatc
    14161 tttcgttaac atgagcaagc tccctttaaa tagtgatgaa tcgcctcagc ggcttctttc
    14221 ccggttttca cggcataaac cacggttttg tcaccctcaa caatatcgcc agcagcaaag
    14281 acttgcgggt cgcgggtctg gtaattttgt gttttgatgg tgttacgctg cggctctaac
    14341 tccgcaaagg catccagtct ggcatgctga ccgacggcga gaataatttt atctgccgcc
    14401 atcgtcagtt cgcccggtaa ccgtacatgc ttaaacgtga ctttattgcc ttccacagct
    14461 actggcgtga atccatcgat gatcgaaacg cccagctccc gggcgctggc aaactctttt
    14521 tcgcttgccg gaaactcatc taactcttca cgcgctacgc aagttactgc ctgacagccg
    14581 agaactttca gcgtgctggc tacgtccatc gcgacatcac cgccgccgat aattaatgcg
    14641 ctttgtggaa tgctgatatc gccttgcgcc tgtcgtgcac gttgcaagaa gtcgacggca
    14701 atctcaacat cgctatgctc aaacagtggt agccccgaac cgctcgataa ccccacagtg
    14761 accagtaccg cgcggttttc cgctttcagc tgctccaggg tgagtgtgtt accgatttcg
    14821 ttattgcatt taatggtcac gcccattttt tcaatacggg cgatttctgc atccagtact
    14881 gattgcggta accggaattg cggaatgccg ttacgcaacc agccaccggg ttgtgcttct
    14941 ttctcataga tcgtgacgtc ataaccctgg tttgtcagtg tcacactggc ctgcaatccg
    15001 gcaggacccg cgccaataat ggcgactttg ccgagcgttt tagtaccggg ctgataaatt
    15061 tccatcccag tttgttgttc aaaatcagta acaaaacgct gtaagcgacc gatatcaatg
    15121 ggagtatcaa caccggcacg ggtacaaccg ctttggcata atttctccgt cgggcacact
    15181 ctggcacaaa cggcaccgag ggcattattt tcacgaattg tctcggcagc gcctttaaaa
    15241 ttacgaaagt agattgagcg aataaatttc cccggatcgg tctgtgccgg gcaagcctga
    15301 ctacaggggg cgtcatggca taataaacag cgagaggctt ctttaatcgc cagtaacggt
    15361 gtaaaagccg gagtgagttc atccagataa ttttgttgcg gcatgtgatc ttccttaatg
    15421 ctatgagtaa ttaaggcgaa cgctaaatat actcgtcata cttcaagttg catgtgctgc
    15481 gtctgcgttc gctcacccca gtcacttact tatgtaagct cctggggact cactcacttg
    15541 tcgc
//
LOCUS       DAZFLD010000117        15231 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.118, whole genome shotgun sequence.
ACCESSION   DAZFLD010000117 DAZFLD010000000
VERSION     DAZFLD010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15231
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.118"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            188..633
                     /locus_tag="ACHKBL_003066"
                     /pseudo
     CDS             188..633
                     /locus_tag="ACHKBL_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611788.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4/IS5 family transposase"
     gene            677..945
                     /locus_tag="ACHKBL_003067"
                     /pseudo
     CDS             677..945
                     /locus_tag="ACHKBL_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009008364.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1150..2268
                     /gene="ybdK"
                     /locus_tag="ACHKBL_003068"
     CDS             1150..2268
                     /gene="ybdK"
                     /locus_tag="ACHKBL_003068"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbdK family carboxylate-amine ligase"
                     /protein_id="HFO5819906.1"
                     /translation="MPIPDFQVSEPFTLGIELEMQVVNPPGYDLSQDSSMLIDAVKNK
                     ITAGEVKHDITESMLELATDVCRDINQAAGQFSAMQKVVLQAAADHHLEICGGGTHPF
                     QKWQRQEVCDNERYQRTLENFGYLIQQATVFGQHVHVGCASGDDAIYLLHGLSRFVPH
                     FIALSAASPYMQGTDTRFASSRPNIFSAFPDNGPMPWVSNWQQFEALFRCLSYTTMID
                     SIKDLHWDIRPSPHFGTVEVRVMDTPLTLSHAVNMAGLIQATAHWLLTERPFKHQEKD
                     YLLYKFNRFQACRYGLEGVITDPHTGDRRSLMEDTLRLLEKIAPSAHKIGASSAIEAL
                     HRQVVSGLNEAQLMRDFVADGGSLIGLVKKHCEIWAGD"
     gene            2334..2582
                     /gene="ybdJ"
                     /locus_tag="ACHKBL_003069"
     CDS             2334..2582
                     /gene="ybdJ"
                     /locus_tag="ACHKBL_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1158 domain-containing protein"
                     /protein_id="HFO5819907.1"
                     /translation="MKHPLETLTTAAGILLMAFLSCLLLPAPALGLTLAQKLVTMFHL
                     MDLSQLYTLLFCLWFLVLGAIEYFVLRFIWRRWFSLAD"
     gene            2647..3015
                     /gene="ybdF"
                     /locus_tag="ACHKBL_003070"
     CDS             2647..3015
                     /gene="ybdF"
                     /locus_tag="ACHKBL_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFO5819908.1"
                     /translation="MDKQSLHETAKRLALELPFVELCWPFGPEFDVFKIGGKIFMLSS
                     ELRGVPFINLKSDPQKSLLNQQIYPSIKPGYHMNKKHWISVYPGEEISEALLRDLIND
                     SWNLVVDGLAKRDQKRVRPG"
     gene            3109..3762
                     /gene="nfsB"
                     /locus_tag="ACHKBL_003071"
     CDS             3109..3762
                     /gene="nfsB"
                     /locus_tag="ACHKBL_003071"
                     /EC_number="1.5.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NAD(P)H nitroreductase"
                     /protein_id="HFO5819909.1"
                     /translation="MDIISVALKRHSTKAFDASKKLTPEQAEQVKTLLQYSPSSTNSQ
                     PWHFIVASTEEGKARVAKSAAGNYVFNERKMLDASHVVVFCAKTAMDDAWLKLVVDQE
                     DADGRFATPEAKAANDKGRKFFADMHRKDLHDDAEWMAKQVYLNVGNFLLGVAALGLD
                     AVPIEGFDAAILDEEFGLEEKGYTSLVVVPVGHHSVEDFNATLPKSRLPQNITLTEV"
     gene            3870..5117
                     /gene="ybdG"
                     /locus_tag="ACHKBL_003072"
     CDS             3870..5117
                     /gene="ybdG"
                     /locus_tag="ACHKBL_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel YbdG"
                     /protein_id="HFO5819910.1"
                     /translation="MQDLISQVEDLAGIEIDHTTSMVMIFGIIFLTAVVVHIFLHWVV
                     LRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGIIVNIQAVFWLQKGTEAADILTTC
                     AQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQGIKLIGAILVGILMISLL
                     IGQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGA
                     VIDIGLTTVKVRNWDNTITTIPTWSLVSDSFKNWSGMSASGGRRIKRSISIDVTSIRF
                     LDEDEMQRLNKAHLLKPYLTSRHQEINEWNRQQGSTESVLNLRRMTNIGTFRAYLNEY
                     LRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQADIFDHIFAIVEE
                     FGLRLHQSPTGNDIRSLAGAFKQ"
     gene            complement(5198..6574)
                     /gene="pheP"
                     /locus_tag="ACHKBL_003073"
     CDS             complement(5198..6574)
                     /gene="pheP"
                     /locus_tag="ACHKBL_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine transporter"
                     /protein_id="HFO5819911.1"
                     /translation="MKNASTVSEDTASNQEPTLHRGLHNRHIQLIALGGAIGTGLFLG
                     IGPAIQMAGPAVLLGYGVAGIIAFLIMRQLGEMVVEEPVSGSFAHFAYKYWGPFAGFL
                     SGWNYWVMFVLVGMAELTAAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGET
                     EFWFALIKVLAIIGMIGFGLWLLFSGHGGEKASIDNLWRYGGFFATGWNGLILSLAVI
                     MFSFGGLELIGITAAEARDPEKSIPKAVNQVVYRILLFYIGSLVVLLALYPWVEVKSN
                     SSPFVMIFHNLDSNVVASALNFVILVASLSVYNSGVYSNSRMLFGLSVQGNAPKFLTR
                     VSRRGVPINSLMLSGAITSLVVLINYLLPQKAFGLLMALVVATLLLNWIMICLAHLRF
                     RAAMRRQGRETQFKALLYPFGNYLCIAFLGMILLLMCTMDDMRLSAILLPVWIVFLFV
                     AFKTLRRK"
     gene            complement(6676..9819)
                     /gene="cusA"
                     /locus_tag="ACHKBL_003074"
     CDS             complement(6676..9819)
                     /gene="cusA"
                     /locus_tag="ACHKBL_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit CusA"
                     /protein_id="HFO5819912.1"
                     /translation="MIEWIIRRSVANRFLVLMGALFLSIWGTWTIINTPVDALPDLSD
                     VQVIIKTSYPGQAPQIVENQVTYPLTTTMLSVPGAKTVRGFSQFGDSYVYVIFEDGTD
                     PYWARSRVLEYLNQVQGKLPAGVSAELGPDATGVGWIYEYALVDRSGKHDLADLRSLQ
                     DWFLKYELKTIPDVAEVASVGGVVKEYQVVIDPQRLAQYGISLAEVKSALDASNQEAG
                     GSSIELAEAEYMVRASGYLQTLDDFNHIVLKASENGVPVYLRDVAKVQIGPEMRRGIA
                     ELNGEGEVAGGVVILRSGKNAREVIAAVKDKLETLKSSLPEGVEIVTTYDRSQLIDRA
                     IDNLSGKLLEEFIVVAVVCALFLWHVRSALVAIISLPLGLCIAFIVMHFQGLNANIMS
                     LGGIAIAVGAMVDAAIVMIENAHKRLEEWQHQHPDATLDNKTRWQVITDASVEVGPAL
                     FISLLIITLSFIPIFTLEGQEGRLFGPLAFTKTYAMAGAALLAIVVIPILMGYWIRGK
                     IPPESSNPLNRFLIRVYHPLLLKVLHWPKTTLLVAALSVLTVLWPLNKVGGEFLPQIN
                     EGDLLYMPSTLPGISAAEAASMLQKTDKLIMSVPEVARVFGQTGKAETATDSAPLEMV
                     ETTIQLKPQDQWRPGMTMDKIIEELDNTVRLPGLANLWVPPIRNRIDMLSTGIKSPIG
                     IKVSGTVLADIDAMAEQIEEVARTVPGVASALAERLEGGRYINVEINREKAARYGMTV
                     ADVQLFVTSAVGGAMVGETVEGIARYPINLRYPQSWRDSPQALRQLPILTPMKQQITL
                     ADVADVKVSTGPSMLKTENARPTSWIYIDARDRDMVSVVHDLQKAIAEKVQLKPGTSV
                     AFSGQFELLERANHKLKLMVPMTLMIIFVLLYLAFRRVGEALLIISSVPFALVGGIWL
                     LWWMGFHLSVATGTGFIALAGVAAEFGVVMLMYLRHAIEAEPSLNNPQTFSEQKLDEA
                     LYHGAVLRVRPKAMTVAVIIAGLLPILWGTGAGSEVMSRIAAPMIGGMITAPLLSLFI
                     IPAAYKLMWLHRHRVRK"
     gene            complement(9831..11054)
                     /gene="cusB"
                     /locus_tag="ACHKBL_003075"
     CDS             complement(9831..11054)
                     /gene="cusB"
                     /locus_tag="ACHKBL_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     adaptor subunit CusB"
                     /protein_id="HFO5819913.1"
                     /translation="MKKIALIIGSMIAGGIISAAGFTWFAKAEPPAEKTSTAERKILF
                     WYDPMYPNTRFDKPGKSPFMDMDLVPKYADEESSASGVRIDPTQTQNLGVKTATVTRG
                     PLTFAQSFPANVSYNEYQYAIVQARAAGFIDKVYPLTVGDKVQKGTPLLDLTIPDWVE
                     AQSEYLLLRETGGTATQTEGILERLRLAGMPEADIRRLIATQKIQTRFTLKAPIDGVI
                     TAFDLRAGMNIAKDNVVAKIQGMDPVWITAAIPESIAWLVKDASQFTLTVPARPDKTL
                     TIRKWTLLPGVDAATRTLQLRLEVDNADESLKPGMNAWLQLNTASEPMLLIPSQALID
                     TGSEQRVITVDADGRFVPKRVAVFQASQGVTALRSGLAEGEKVVSSGLFLIDSEANIS
                     GALERMRSESATHAH"
     gene            complement(11070..11402)
                     /gene="cusF"
                     /locus_tag="ACHKBL_003076"
     CDS             complement(11070..11402)
                     /gene="cusF"
                     /locus_tag="ACHKBL_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     metallochaperone CusF"
                     /protein_id="HFO5819914.1"
                     /translation="MKKALQVAMFSLFTVIGFNAQANEHHHETMSEAQPQVISATGVV
                     KTIDLESKKIIIHHDPITAVNWPEMTMRFTITPQTKMSEIKTGDKVAFNFVQQGNLSL
                     LQDIKVSQ"
     gene            complement(11426..12808)
                     /gene="cusC"
                     /locus_tag="ACHKBL_003077"
     CDS             complement(11426..12808)
                     /gene="cusC"
                     /locus_tag="ACHKBL_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter outer
                     membrane channel CusC"
                     /protein_id="HFO5819915.1"
                     /translation="MSPCKLLPFCVALALTGCSLAPDYQRPAMPVPQQFSLNQNGLVN
                     AADNYQNAGWRTFFVDNQVKTLISEALVNNRDLRMATLKVQEARAQYRLTDADRYPQL
                     NGEGSGSWSNNLKGDSATTREFSTGLNASFDLDFFGRLKNMSEAERQNYLATEEAQRA
                     VHILLVSNVAQSYFNQQLAYAQLQIAEETLRNYQQSYAFVEKQLLTGSSNVLALEQAR
                     GVIESTRSDIAKRQGELAQANNALQLLLGSYGKLPQAQTVNSDSLQSVKLPAGLSSQI
                     LLQRPDIMEAEHALMAANANIGAARAAFFPSISLTSGISTASSDLSSLFNASSGMWNF
                     IPKIEIPIFNAGRNQANLDIAEIRQQQSVVNYEQKIQNAFKEVADALALRQSLNDQIS
                     AQQRYLASLQITLQRARALYQHGAVSYLEVLDAERSLFATRQTLLDLNYARQVNEISL
                     YTALGGGWQQ"
     gene            12965..13648
                     /gene="cusR"
                     /locus_tag="ACHKBL_003078"
     CDS             12965..13648
                     /gene="cusR"
                     /locus_tag="ACHKBL_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper response regulator transcription factor
                     CusR"
                     /protein_id="HFO5819916.1"
                     /translation="MKLLIVEDEKKTGEYLTKGLTEAGFVVDLADNGLNGYHLAMTGD
                     YDLIILDIMLPDVNGWDIVRMLRSANKGMPILLLTALGTIEHRVKGLELGADDYLVKP
                     FAFAELLARVRTLLRRGAAVIIESQFQVADLMVDLVSRKVTRSGTRITLTSKEFTLLE
                     FFLRHQGEVLPRSLIASQVWDMNFDSDTNAIDVAVKRLRGKIDNDFEPKLIQTVRGVG
                     YMLEVPDGQ"
     gene            13638..15086
                     /gene="cusS"
                     /locus_tag="ACHKBL_003079"
     CDS             13638..15086
                     /gene="cusS"
                     /locus_tag="ACHKBL_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) sensor histidine kinase CusS"
                     /protein_id="HFO5819917.1"
                     /translation="MVSKPFQRPFSLATRLTFFISLATIAAFFAFAWIMIHSVKVHFA
                     EQDINDLKEISATLERVLNHPDETQAQRLMTLEDIVSGYSNVLISLADSHGKTVYHSP
                     GAPDIREFTRDAIPDKDTRGGEVYLLSGPTMMMPGHGHGHMEHSNWRMISLPVGPLVD
                     GKPIYTLYIALSIDFHLHYINDLMNKLIMTASIISILIVFIVLLAVHKGHAPIRSVSR
                     QIQNITSKDLDVRLDPQTVPIELEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTP
                     ITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVSDMLFLAQADNNQLIPEKK
                     MLNLADEVGKVFDFFEALAEDRGVELRFVGDECQVAGDPLMLRRALSNLLSNALRYTP
                     PGEAIVVRCQTVDHQVQVTVENPGTPIAPEHLPRLFDRFYRVDPSRQRKGEGSGIGLA
                     IVKSIVVAHKGTVAVTSDARGTRFMIKLPEKG"
BASE COUNT         3692 a         4081 c         3738 g         3720 t
ORIGIN      
        1 aataaaaaat aagacattac gataatatta taaagcattg ctttgttggg ggatgacgta
       61 tgtagcaatg aaagataaca aatgccggta atggtacgga gaaatcctca gtaataggcg
      121 ggtaaaaaag acgggcatag agttttgtgg tcgactggtt gtgctaacaa ttcaggagat
      181 cacctctatg cccgttcgct catcatgcca aaagtttttc cgtgatgatt tgactccatt
      241 tcatcagtac cgccagaatg ccatccttga cgccactgct gcgcttagtc gggacgcatc
      301 cctcatcctt accagcattg gacggtatat gccaggcaat gctcaggtta aaacaagatt
      361 aagcccatcg atcgcctgcc aggcaatgac gctttacacc gcgatatccc ggctattttt
      421 cataatatta cccggtttca gccccgagac cttacgttgt gcgtcattgc cgttgactgg
      481 agtggttatc cttctcaggc attccatgtt ctgcatactc cccaagtttg cgacgggcgt
      541 tctatattac ttctggatca ggttgtatcg tcattctggc aacaaattgc tcagttctat
      601 aagaattttc ttgatacgct tgcaatcact attgctccga ataaaccgga taaaaattac
      661 ctttaaacac agcaccatga ccgccacaag ggcatatttc gtcagcatgt ttatattgcc
      721 tccatgcaaa gaagggacta ccatccaaat tgtttgggtt tggagtggca gccccaggat
      781 tgatagaaat atctcctggg gcttcatctt tccagacctc agagtaaacc tgaaaccgga
      841 aagcttcagg cacccgccgt tatcttaccc ttccttagca aaatggctgt ttatttatac
      901 agtaagtcct gtttcggact tctcaatcct catttgatca actgaagctg aacccgtttt
      961 attctgcatg cctcaatcgt caaaattctt cgccaccagt ccgcactgcc ataacttgtc
     1021 atattgcaaa atgtagccaa tttgcttcag tcaccatcat caaaaaaaga aacattccag
     1081 aacttccgca tctcgcccag ccgctccgac ttctatactg aatagaaaac gccaacataa
     1141 gagaaaccta tgccaatacc cgattttcaa gtctctgaac cttttaccct cggtattgaa
     1201 ctggaaatgc aggtggttaa tccgccgggc tatgacttaa gccaggactc ttcaatgctg
     1261 attgacgcgg ttaaaaataa gatcacggcc ggagaggtaa agcacgatat caccgaaagt
     1321 atgctggagc tggcgacgga tgtttgccgt gatatcaacc aggctgccgg gcaattttca
     1381 gcgatgcaga aagtcgtatt gcaggcagcc gcagaccatc atctggaaat ttgcggcggt
     1441 ggcacgcacc cgtttcagaa atggcagcgt caggaggtat gcgataacga acgctatcaa
     1501 cgaacgctgg aaaactttgg ctatctcatc cagcaggcga ccgtttttgg tcagcatgtc
     1561 catgttggct gcgccagtgg cgatgatgcc atttatttgc tgcacggcct gtcacggttt
     1621 gtgccgcact ttatcgccct ttccgccgcg tcgccatata tgcaaggaac ggatacgcgt
     1681 tttgcctcct cacgaccgaa tattttttcc gcctttcccg ataatggccc gatgccgtgg
     1741 gtcagtaact ggcaacaatt tgaagccctg tttcgctgct tgagttacac cacgatgatc
     1801 gacagcatta aagatctgca ctgggatatt cgccccagtc ctcattttgg cacggtggag
     1861 gttcgggtaa tggatacccc gttaaccctt agccatgcgg taaatatggc gggattaatt
     1921 caggctactg cccactggtt actgacagaa cgcccgttca aacatcagga gaaagattac
     1981 ctgctgtata aattcaaccg tttccaggcc tgtcgctatg ggctggaagg cgtcattacc
     2041 gatccgcata ctggcgatcg tcggtcacta atggaagaca ccttgcgttt actggaaaaa
     2101 atcgcccctt ctgcacataa aattggcgca tcgagcgcaa tagaagcact gcaccgccag
     2161 gtcgtcagcg gtctgaatga agcgcagctg atgcgcgatt tcgtcgccga tggcggctcg
     2221 ctgattgggc tggtgaaaaa gcattgtgag atctgggctg gtgactaagc cgggaattgc
     2281 cttacagaag aatatccctg acaatggtgt tttaacttac atttaacaat gttatgaaac
     2341 accctttaga aaccttgacc accgcagcag gcattttgct gatggctttc ctttcttgcc
     2401 tgctgctgcc cgcccccgca ctggggctga cgctggcaca aaaactggtg accatgttcc
     2461 atctgatgga tcttagccag ctttacactt tattgttttg tctgtggttt ttagtgctgg
     2521 gcgctattga gtattttgtt ctgcgcttta tctggcgacg ctggttctcg ctggcggatt
     2581 aaacgtcgat tatcgccgta cttcgcggca tactttgctt attctccttc gcctaaagga
     2641 acgtttatgg ataagcaatc actgcacgaa acggcgaaac gcctggccct tgagttaccc
     2701 tttgtcgagc tttgctggcc ttttggcccg gagttcgatg tttttaaaat tggcggcaag
     2761 atttttatgc tgtcgtcgga gctgcgcggc gtccccttta tcaatctgaa gtccgatcca
     2821 caaaaatccc tgttaaatca gcaaatatac ccgagcatca agccagggta tcacatgaat
     2881 aaaaagcact ggatttcagt gtatcccggc gaggaaatct ccgaagcgtt acttcgcgat
     2941 ctgatcaacg attcgtggaa tctggtggtt gatggtctgg ctaaacgcga tcaaaaaaga
     3001 gtgcgtccag gctaaagcgg aaatctatag cgcatttttc tcgcttacca tttctcgttg
     3061 aaccttgtaa tctgctggca cgcaaaatta ctttcacatg gagtctttat ggatatcatt
     3121 tctgtcgcct taaagcgtca ttccactaag gcatttgatg ccagcaaaaa actaaccccg
     3181 gaacaggccg agcaggtcaa aactctcctg caatacagcc catccagtac caactcccag
     3241 ccgtggcatt ttattgttgc cagcacggaa gaaggtaaag cgcgtgttgc caaatccgct
     3301 gccggtaatt acgtgttcaa cgagcgtaaa atgcttgatg cctcgcacgt cgtggtgttc
     3361 tgtgcaaaaa ccgcgatgga cgatgcctgg ctgaagctgg ttgttgacca ggaagatgcc
     3421 gatggccgct ttgccacgcc ggaagcgaaa gccgcgaacg ataaaggtcg caagttcttc
     3481 gccgatatgc accgtaaaga tctgcacgat gatgcagagt ggatggcaaa acaggtttat
     3541 ctcaacgtcg gtaacttcct gctcggcgtg gcggcgctgg gtctggacgc ggtgcccatc
     3601 gaaggttttg acgccgccat cctcgatgaa gaatttggtc tggaagagaa aggctacacc
     3661 agtctggtgg ttgttccggt gggtcatcac agcgttgaag attttaacgc tacgctgccg
     3721 aaatctcgtc tgccgcaaaa cattacctta accgaagtgt aattctccct tgccgggcat
     3781 ctgcccggct atttcccctc agattctcct gatttgcata accctgtttc agccgtcatc
     3841 ataggctgct gttgtataaa ggagacgtta tgcaggattt aatatcccag gttgaagatt
     3901 tagcgggtat tgagatcgat cacaccacct cgatggtgat gattttcggt attatttttc
     3961 tgaccgccgt cgtggtgcat atttttttgc actgggtggt actgcggacc ttcgaaaaac
     4021 gcgccatcgc cagttcacgg ctttggttgc aaatcattac ccagaataaa ctcttccacc
     4081 gtttagcttt taccctgcag gggattatcg tcaatattca ggcggtattc tggctgcaaa
     4141 aaggcaccga agcggcagat attctgacta cctgcgcgca gttgtggatc atgatgtatg
     4201 cgctgctttc agtcttctcg ttgctggatg tgattttgaa tctggcgcag aaattcccgg
     4261 cggcatctca gttaccgctg aaagggatat ttcaggggat taaactgatc ggcgcgattc
     4321 tggtcggcat tttgatgatc tcgctgctga ttggtcagtc gccagcgatt ctgatcagcg
     4381 gtcttggtgc aatggctgcc gtgctgatgt tggtatttaa agatccgatt cttggtctgg
     4441 tggcaggtat tcagctttcc gcgaacgata tgctgaaact gggcgactgg ctggagatgc
     4501 cgaaatacgg cgcggatggc gcggtgatcg atattgggtt aaccaccgtc aaagtacgta
     4561 actgggacaa taccattacc actattccca cctggtctct ggtttctgac tcctttaaaa
     4621 actggagcgg gatgtcagca tctggcgggc gacgtattaa gcgcagtatc agtattgatg
     4681 tcaccagtat tcgttttctt gatgaagacg aaatgcaacg tctgaataaa gcgcatttgt
     4741 taaagcctta tttaaccagc cgccatcagg aaattaatga gtggaatcgc cagcaaggtt
     4801 ctacggagtc ggtattaaac ctgcgccgaa tgaccaatat tggaaccttt cgtgcctatc
     4861 tgaacgaata tctacgtaac catccgcgga ttcgtaaaga tatgacctta atggtacgcc
     4921 aactggctcc tggcgataac ggtttaccgc tcgagatcta tgcgtttacc aacaccgtgg
     4981 tgtggctgga atatgaaagc attcaggctg atatattcga ccacatattt gcgattgtcg
     5041 aagagtttgg tctgcgactt catcagtcgc caactggcaa tgatattcgc tctctggcgg
     5101 gtgcatttaa gcagtaatta aaaaaaccgc tctcatcgaa tggatgagag cggtttcgga
     5161 tggttgacat cattttgtcg gatacggcgc gaatgcctta tttccgacgc agcgttttaa
     5221 acgctacaaa caggaataca atccacactg gcagcagaat cgctgacaag cgcatatcat
     5281 ccatcgtgca catcagcagc aaaatcatgc cgaggaaggc gatgcaaaga tagttgccga
     5341 acggatagag cagcgcctta aactgtgttt cacgtccctg acgtcgcatc gccgcgcgaa
     5401 aacgcagatg cgccaggcag atcataatcc agttcaacag cagcgttgct accaccagcg
     5461 ccatcagcag accaaacgct ttttgcggca gcagatagtt aatcagcacc accagcgaag
     5521 tgatggctcc ggaaagcatc agcgagttaa tcggcacgcc gcgacggctg acgcgagtca
     5581 aaaacttcgg tgcattaccc tgcacagaaa ggccaaacag catgcggctg ttagagtaaa
     5641 ccccgctgtt atacaccgac agcgatgcca ccagaatgac gaagttcagc gcagaagcta
     5701 ccacgttgct gtcgagatta tggaaaatca tcacaaacgg gctactgttg gatttcactt
     5761 ccacccacgg atagagtgcc agtaaaacca ccagtgaacc gatataaaac agcagaatgc
     5821 gatacaccac ctgattcact gctttcggaa tgcttttttc cggatcgcgc gcttcagcgg
     5881 cagtaatccc aatcagctcc agaccgccga aagagaacat aattaccgcc agcgacaaaa
     5941 tcagcccatt ccagccggtg gcaaaaaaac caccgtaacg ccagaggtta tcgatactgg
     6001 ctttctcgcc gccgtgacca gaaaacagca gccacaggcc aaagccgatc ataccgatga
     6061 tcgccagcac tttaatcagc gcaaaccaga attcggtttc gccatataag cgcacgttca
     6121 ccaggttaac ggcgttgatg ataataaaga aggcggcagc ccaaatccac gttggaacat
     6181 ccgggaacca gtactgcata tagatgcccg cagcggtcag ctctgccatt cccaccagca
     6241 cgaacattac ccagtagttc cagccagaga ggaagcccgc aaacggtccc cagtatttat
     6301 aggcaaagtg ggcaaatgaa ccggataccg gctcctcaac caccatttcg ccaagctggc
     6361 gcataatcag gaaagcgatg atcccggcga cgccgtagcc cagcaataca gccggacccg
     6421 ccatctgaat cgccggacca atgccaagaa acagaccagt accaattgcg ccacccagcg
     6481 caatcagttg aatatgacgg ttatgtaatc cgcgatgaag cgtcggctct tgattcgacg
     6541 cagtatcttc cgatacggtt gacgcgtttt tcacgccttt cccctgtgtg tcttttttgt
     6601 tgaggggcac cttttaacat ttagtgccca tcgtcgcaag acacaatcca cacggttaaa
     6661 cggggtatcc tgcttttatt tccgtacccg atgtcggtgc agccacatca gcttatacgc
     6721 cgccgggata ataaacagcg acagcaaagg tgcggtgatc atgccgccaa tcatcggtgc
     6781 ggcaatccgg ctcatcacct ctgaaccagc ccccgttccc cacagaatcg gcagcagacc
     6841 ggcgataatc actgccaccg tcatcgcttt cgggcgcacg cgcaggaccg cgccgtgata
     6901 caacgcctca tccagcttct gctcgctgaa tgtttgcgga ttattcaacg acggctcggc
     6961 ctctatggcg tgacgtaaat acatcagcat caccacgcca aattcggcgg caaccccggc
     7021 aagggcaata aaaccggtgc ccgtcgccac ggaaagatga aagcccatcc accacagcaa
     7081 ccagatgccg cccaccagcg caaacggtac gctgctgata atcagcaacg cttcgcccac
     7141 ccgacggaac gccagatata acagcacgaa gatgatcatc agcgtcatcg gcaccatgag
     7201 tttcagctta tggttggcgc gctccagtag ctcgaactgc ccggagaatg ccacgctggt
     7261 gcccggtttt aactgcactt tttcagctat cgctttttgc agatcgtgaa ccaccgacac
     7321 catgtcacga tcgcgggcat cgatataaat ccagctcgtc gggcgcgcat tctcggtttt
     7381 cagcatcgac ggtccggtag agactttaac gtcagccacg tctgccaggg tgatttgctg
     7441 cttcatcggc gtgaggatcg gcaactggcg cagtgcctgc gggctatcgc gccagctttg
     7501 cggataacgc agattaattg gataacgagc aatcccttcc accgtttcgc caaccatcgc
     7561 cccgcccacc gcagaagtca caaacaactg cacatccgcc accgtcatac cgtaacgcgc
     7621 ggctttttca cggttaatct caacgttgat ataacgccca ccttccagac gctcagcaag
     7681 cgcagaagcc acgcctggca ccgttcgcgc cacttcttca atttgctccg ccatcgcgtc
     7741 gatatccgcc agcacggtgc cggaaacttt aataccgatg ggacttttaa tgccagtcga
     7801 gagcatatcg atacggttac gaattggcgg cacccacaga ttcgccagcc ccggcaaacg
     7861 cacggtgtta tccagttcct caatgatttt gtccatcgtc atgccaggcc gccactgatc
     7921 ctgcggctta agctgaatgg tcgtttctac catctccagc ggagcagaat cggtggcggt
     7981 ttccgctttc ccggtttggc caaatacccg cgccacttcc ggtacgctca taatcagctt
     8041 gtcggttttt tgcagcatac tcgccgcctc cgctgcggaa atccccggca gcgtcgatgg
     8101 catatacaac aagtcgcctt cattgatctg cggtaaaaat tccccgccaa ctttattgag
     8161 cggccagaga accgtcagca ccgaaagcgc tgccaccaac agcgtggttt tcggccagtg
     8221 cagtactttc agcaacagcg gatgataaac acgaatcaaa aagcgattga gcgggttact
     8281 gctttccggc ggaattttgc cacggatcca gtagcccatc aggatcggga tcactacgat
     8341 cgccagtagc gccgcacccg ccatcgcata ggttttggtg aacgccagcg ggccaaacag
     8401 acgcccttcc tgcccttcca gggtgaagat cgggataaac gacagcgtga taatcagcag
     8461 actgataaac agcgccggcc ccacttcaac agatgcatcg gtaatcacct gccagcgcgt
     8521 tttattatcc aacgtggcgt caggatgctg gtgctgccac tcttccagcc gtttatgcgc
     8581 attctcgatc atgacgatag cggcatcgac catcgccccg acggcaatcg caatgccacc
     8641 caacgacata atattggcgt tcagtccctg aaagtgcatg acaataaaag caatacacaa
     8701 ccccagcggc aacgaaataa tcgccaccag cgccgagcgc acatgccaga gaaacagcgc
     8761 acagactacc gccaccacaa taaactcttc cagcaacttg ccgctgaggt tgtcgatggc
     8821 gcgatcaatg agctggctgc gatcgtatgt tgtaactatc tccacgcctt ccggcagact
     8881 acttttcagc gtttccagtt tgtccttcac ggcggcgatt acttctcggg cgtttttgcc
     8941 ggagcgcagg atcaccaccc cgcccgccac ttcgccttcg ccgttcagtt cggcaatgcc
     9001 ccggcgcatc tccgggccaa tctggacctt cgcaacatca cgcagataaa cgggcacgcc
     9061 attttcactg gcttttaaaa cgatgtgatt aaagtcgtcg agcgtttgca gatagccgct
     9121 ggctcgcacc atatattccg cttccgccag ttcgatcgac gaaccacccg cttcctggtt
     9181 tgaagcatcc agcgcgcttt ttacttcggc gagactgatg ccatattgcg ccaggcgctg
     9241 aggatcgata accacctgat actctttcac cacaccgcct accgacgcca cttccgcaac
     9301 gtcaggaatg gttttcagct catatttgag aaaccagtcc tgcaatgagc gtaaatcggc
     9361 aagatcgtgc ttaccgctgc gatccaccag tgcatattca tagatccagc caacacccgt
     9421 ggcatctggc cccagctcgg cgctgactcc cgcaggcagc ttaccctgta cctggttgag
     9481 gtactccagc acccgcgagc gcgcccagta cggatcggtg ccatcttcga aaatgacata
     9541 cacataagag tcgccaaact gcgagaaacc gcgcacagtc ttcgcgccag gcaccgacaa
     9601 catggtggtg gttagcggat aagtcacctg attttcaacg atttgcggtg cctgaccggg
     9661 atagctggtt ttaataatca cctgcacatc agagagatcc ggcagtgcat ccactggcgt
     9721 gttaatgatg gtccaggtgc cccagatact cagaaacaac gcgcccatca gcaccagaaa
     9781 acggttcgcc accgagcgac gaataatcca ttcaatcatt ggttattccc tcaatgcgca
     9841 tgggtagcac tttcagagcg catccgctcc agtgcgccag aaatattggc ttcggaatca
     9901 atcaggaaca ggccgctgga aaccaccttt tcaccttccg ccagaccaga gcgtaacgcg
     9961 gtgacgcctt gcgatgcctg gaaaacggca acgcgtttcg gtacaaagcg tccgtcggca
    10021 tcaacggtaa tcacccgctg ttcactgccg gtatcaatca gcgcctgtga cggaatgagc
    10081 agcatcggtt cgctggcggt attgagttgc aaccaggcgt tcatgcccgg ttttagcgac
    10141 tcgtcggcgt tgtcgacttc cagacgcagc tgcagcgtgc gggtcgcggc atccacgccg
    10201 ggtagcaggg tccatttgcg aatggtgagt gttttatcag gtcgcgccgg aacggtgagc
    10261 gtaaactgcg aggcatcttt caccagccat gcgatggact ccgggatcgc ggcagtgatc
    10321 cacaccgggt ccataccctg aattttcgcg accacgttat ctttggcgat attcattccc
    10381 gcgcgcagat caaacgcggt gatcacgcca tcaatgggtg ctttgagcgt aaagcgagtc
    10441 tggatttttt gcgtggcaat caggcggcga atatccgcct ccggcattcc cgccagtcgc
    10501 agtcgctcaa gaatgccttc agtctgggtc gccgtaccgc cggtttcgcg cagcagtaaa
    10561 tactcactct gcgcttccac ccagtcggga atggtcaggt cgagaagcgg tgtgcccttt
    10621 tgtactttat cgcccacggt aagcggatac accttatcga taaacccggc agcgcgagcc
    10681 tgcacaatgg catactgata ctcgttgtaa ctgacattcg ccgggaaact ctgggcaaaa
    10741 gtcagcggcc cgcgcgttac ggtcgccgtt ttcaccccca gattctgcgt ctgggtcggg
    10801 tcaatgcgta caccagacgc agaactctct tcatcggcat atttcggcac cagatccata
    10861 tccataaacg gcgatttacc tggtttatcg aaccgcgtat tgggatacat cgggtcgtac
    10921 cagaataaga ttttacgttc tgcggtcgac gttttttctg cgggcggttc cgcctttgca
    10981 aaccaggtaa aacctgccgc agaaataata ccgcccgcga tcatgctgcc gataataagc
    11041 gcgatttttt tcatctcatt aaacctgggt tactggctga ctttaatatc ctgtaataaa
    11101 gaaaggttgc cctgctggac aaaattaaac gccactttgt cgccggtttt aatttcactc
    11161 attttcgtct gcggggtgat ggtaaagcgc atggtcatct ccggccagtt caccgcggta
    11221 attggatcgt ggtggatgat gatttttttg ctttccagat caatggtttt taccacaccg
    11281 gtggcgctaa tgacctgtgg ttgtgcctcg ctcatggttt catgatgatg ttcgttagcc
    11341 tgggcattaa agccaataac ggtaaacaga ctaaacattg cgacttgcag tgcttttttc
    11401 atttattctc tcctggagtt aaaaattatt gctgccaacc gccgcctaag gcggtatata
    11461 aagaaatttc attaacctga cgggcataat tcaggtcaag taaagtttgt cgggttgcaa
    11521 ataaagaacg ctcagcatcc agcacttcca gataacttac cgcgccgtgc tgatataatg
    11581 cccgcgcccg ttgcaaagta atttgcagcg acgccagata acgctgctgg gcgctaattt
    11641 gatcgttcag gctttgacgt aatgccagcg catctgccac ttctttaaag gcgttctgga
    11701 ttttctgttc ataattcacc accgactgct gctggcgaat ttcggcgata tccagattgg
    11761 cctggttacg tccggcatta aaaatgggga tctcaatttt gggtataaaa ttccacatcc
    11821 cgctgctggc gttaaataat gacgacagat cgctgctggc ggttgatatt ccgctggtca
    11881 ggctgataga cgggaaaaat gccgcgcgtg cagcaccaat attggcatta gccgccatta
    11941 acgcatgttc agcttccata atatccgggc gctgcaataa gatttgcgac gacaagcccg
    12001 ccggtaattt aacgctttgc aggctgtcgc tgtttactgt ctgcgcttgc ggcagcttgc
    12061 cgtagcttcc caataacagt tgcaatgcat tattcgcctg cgccagttcc ccctgacgtt
    12121 tagcgatgtc gctgcgggta ctttcaatca ccccgcgagc ctgttccagc gccagaacat
    12181 tactgctacc tgtcaacagt tgtttttcga caaacgcata tgactgctga taattacgca
    12241 gcgtttcttc ggctatttgc aattgcgcat acgccagctg ctgattgaaa tagctttgcg
    12301 cgacattaga aaccagcaaa atatgcaccg cgcgctgagc ttcctcagtg gctaaataat
    12361 tttggcgctc ggcttcgctc atgttcttta agcgcccgaa aaaatcgaga tcaaagctgg
    12421 cgttaaggcc agtcgagaac tcccgcgtcg tggctgaatc gcctttaaga ttgttgctcc
    12481 agctgccgct gccctcgcca ttgagctgtg ggtagcggtc ggcatcggtc agacgatatt
    12541 gcgcccgcgc ttcctgcact ttcagcgtcg ccatgcgcaa atcccggtta ttcaccagcg
    12601 cctcgctaat cagcgtcttc acctgattat caacaaaaaa ggtgcgccag cccgcgttct
    12661 ggtagttatc tgctgcgtta accaggccgt tctggttgag tgagaactgc tgcggcacgg
    12721 gcattgccgg gcgctgataa tccggtgcca gtgaacaacc ggttagcgca agggccacac
    12781 aaaatggcag aagtttacaa ggagacatag gctcataatt tctggtgatt ttatgccgcc
    12841 aactttactc gccaggctct gattttccgg tgacaggaaa atgacaaaat tgtcattttg
    12901 ccaataagcg attgccatct gatcccgcta ctctagaatt gcccgggcaa catgcggagg
    12961 aaatatgaaa ctgttgattg tcgaagatga aaagaaaacc ggagaatact tgaccaaagg
    13021 gttaaccgaa gccggttttg tggtcgattt ggccgacaac gggctgaatg gctaccatct
    13081 ggcgatgacc ggtgattatg atctgataat cctcgatatt atgctgccgg acgtgaacgg
    13141 ctgggatatc gtgcgcatgt tgcgttccgc caataagggg atgccgattc tgttgcttac
    13201 cgcgcttggc accattgaac atcgcgtcaa ggggctggag ttgggggcgg atgactatct
    13261 ggtgaagccg ttcgcttttg ctgaactgct ggcgcgagtg cgcactctac tgcggcgcgg
    13321 ggcggcggtg attatcgaaa gtcagtttca ggttgccgat ctgatggtcg atctcgtcag
    13381 ccgcaaagtc acccgcagtg gcacgcgcat cactctgacc agtaaagaat ttactctgct
    13441 ggagtttttc cttcgccatc agggcgaagt actgccccgc tcacttattg cctcacaggt
    13501 gtgggacatg aatttcgaca gcgacactaa cgccattgat gtggcggtga agcggctgcg
    13561 tggcaaaatc gacaacgact ttgagccgaa gctgattcaa accgtgcgcg gcgtgggtta
    13621 catgcttgag gtgccggatg gtcagtaagc cgtttcagcg cccgttttcg ctggcaaccc
    13681 gcctgacctt ttttattagc ctggccacca tcgcggcgtt tttcgccttt gcatggatca
    13741 tgatccactc agtaaaagtg cattttgccg agcaggatat taatgattta aaagagatta
    13801 gcgccactct tgaacgggta cttaatcacc ctgacgaaac gcaagcccaa cgcttaatga
    13861 cgctggaaga tatcgtcagt ggttattcca atgtgttgat ttccctggca gatagccacg
    13921 gtaaaacggt gtatcactcc cccggtgcgc cggatatccg cgagtttacg cgtgacgcca
    13981 tacccgataa agacacgcgg ggcggtgagg tgtatctcct ttccggcccg acgatgatga
    14041 tgccgggcca cggtcacggg catatggaac acagcaactg gcggatgatt agcttgccgg
    14101 ttggcccgtt ggtggacggc aaaccgattt atacgctcta cattgcgctt tcgatcgatt
    14161 ttcatcttca ttacataaat gatttgatga ataaacttat tatgaccgca tcgatcatca
    14221 gcatcctgat cgtctttatc gtgctgttgg cggtacataa aggtcacgcg ccgatccgca
    14281 gcgtcagccg tcaaatccag aacattacct cgaaagatct cgacgttcgc cttgacccgc
    14341 agaccgtgcc cattgagctg gaacagctgg tactgtcgtt caaccatatg atcgagcgta
    14401 ttgaggatgt ctttacccgc cagtccaatt tctcagcgga catcgcccac gaaattcgta
    14461 caccaattac gaatctcata acgcaaacgg aaatcgccct cagccagtca cgcagccaga
    14521 aggagctgga agatgtgctc tactctaatc tcgaagagct gacgcgaatg gcgaaaatgg
    14581 tcagcgatat gctgtttctc gcccaggccg ataacaacca gttaatcccc gaaaagaaaa
    14641 tgctcaacct ggcggatgaa gtcggcaaag tgttcgattt tttcgaggca ttagcggaag
    14701 atcgcggcgt ggagttgcgg tttgttggtg acgagtgtca ggttgcgggc gatccgctga
    14761 tgctgcgtcg ggcgttaagc aacctgcttt cgaacgccct gcgttatacg ccacccggag
    14821 aggcaattgt tgtgcgctgc caaacggtcg atcaccaggt gcaagttacc gtcgaaaacc
    14881 ccggtacgcc cattgcgccc gagcacttac cgcgattgtt tgaccgtttc tatcgcgttg
    14941 acccctcccg tcagcgaaaa ggcgaaggta gcggtattgg cctggcgata gtgaaatcga
    15001 ttgttgtcgc gcataaaggc acggttgcag taacgtcaga tgctcggggg acaaggttta
    15061 tgatcaaact accggagaaa ggttaattag ttagttcaaa ctcaaagagt gatgtatcgc
    15121 cttctgttat tatttatcaa taaaacaggg aagaccttat tggtcgtctg aaatcgaagg
    15181 ttattcattt tcggatattt acgagatatt tagatatgca atttaattaa a
//
LOCUS       DAZFLD010000118        15221 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.119, whole genome shotgun sequence.
ACCESSION   DAZFLD010000118 DAZFLD010000000
VERSION     DAZFLD010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15221
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.119"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(277..352)
                     /locus_tag="ACHKBL_003080"
     tRNA            complement(277..352)
                     /locus_tag="ACHKBL_003080"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(317..319),aa:Phe,seq:gaa)
     gene            complement(458..1165)
                     /gene="yqgA"
                     /locus_tag="ACHKBL_003081"
     CDS             complement(458..1165)
                     /gene="yqgA"
                     /locus_tag="ACHKBL_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HFO5819918.1"
                     /translation="MVIGPFINASAVLLGGVLGALLSQRLPERIRVSMTSIFGLASLG
                     IGILLVVKCANLPAMVLATLLGALIGEICLLEKGVNTAVTKAQNLFRHSRKKPAHESF
                     IQNYVAIIVLFCASGTGIFGAMNEGMTGDPSILIAKSFLDFFTAMIFACSLGIAVSVI
                     SIPLLIIQLTLAWAAALILPLTTPSMMADFSAVGGLLLLATGLRICGIKMFPVVNMLP
                     ALLLAMPLSAAWTAWFA"
     gene            1318..1395
                     /locus_tag="ACHKBL_003082"
     CDS             1318..1395
                     /locus_tag="ACHKBL_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5819919.1"
                     /translation="MHATSLHSLAYRRFARNSSLFLLMR"
     gene            1558..3693
                     /gene="speC"
                     /locus_tag="ACHKBL_003083"
     CDS             1558..3693
                     /gene="speC"
                     /locus_tag="ACHKBL_003083"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HFO5819920.1"
                     /translation="MKSMNIAASSELVSRLSTHRRVVALGDTDFTDVAAVVITAADSR
                     SGILALLKRTGFHLPVFLYSEHVVELPAGVTAVINGNEQQWLELESAACQYEENLLPP
                     FYDTLTQYVEMGNSTFACPGHQHGAFFKKHPAGRHFYDFFGENVFRADMCNADVKLGD
                     LLIHEGSAKDAQKFAAKVFHADKTYFVLNGTSAANKVVTNALLTRGDLVLFDRNNHKS
                     NHHGALIQAGATPVYLEASRNPFGFIGGIDAHCFNEEYLRQQIRDVAPEKADLPRPFR
                     LAIIQLGTYDGTVYNARQVIDTVGHLCDYILFDSAWVGYEQFIPMMADSSPLLLELNE
                     NDPGIFVTQSVHKQQAGFSQTSQIHKKDNHIRGQARFCPHKRLNNAFMLHASTSPFYP
                     LFAALDVNAKIHEGESGRRLWAECVALGIEARKAILARCKLFRPFIPPVVDGKLWQDY
                     PTSVLASDRRFFSFEPGAKWHGFEGYAAEQYFVDPCKLLLTTPGIDAETGEYSDFGVP
                     ATILAHYLRENGIVPEKCDLNSILFLLTPAESHEKLAQLVAMLAQFEQHIEDDSPLAE
                     VLPSVYNKYPVRYRDYTLRQLCQEMHDLYVSFDVKDLQKAMFRQQSFPSVVMNPQDAH
                     SAYIRGEVELVRIRDAEGRIAAEGALPYPPGVLCVVPGEVWGGAVQRYFLALEEGVNL
                     LPGFSPELQGVYSETDADGMKRLFGYVLK"
     gene            complement(3743..4999)
                     /gene="nupG"
                     /locus_tag="ACHKBL_003084"
     CDS             complement(3743..4999)
                     /gene="nupG"
                     /locus_tag="ACHKBL_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupG"
                     /protein_id="HFO5819921.1"
                     /translation="MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVY
                     SSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGAITLFMAAQVTTPEAMFLVILIN
                     SFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHM
                     QLYIGAALSAILVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSM
                     LLGAELQITNMFGNTFLHSFDKDPMFASSFIVQHASIIMSISQISETLFILTIPFFLS
                     RYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVE
                     KEVSPAIRASAQGMFLMMTNGFGCILGGIVSGKVVEMYTQNGITDWQTVWLIFAGYSV
                     VLAFAFMAMFKYKHVRVPTGTQTVSH"
     gene            complement(5201..6280)
                     /gene="mltC"
                     /locus_tag="ACHKBL_003085"
     CDS             complement(5201..6280)
                     /gene="mltC"
                     /locus_tag="ACHKBL_003085"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HFO5819922.1"
                     /translation="MKKYLALALIAPLLISCSTTKKGDTYNEAWVKDTNGFDILMGQF
                     AHNIENIWGFKEVVIAGPKDYVKYTDQYQTRSHINFDDGTITIETIAGTEPAAHLRRA
                     IIKTLLMGDDPSSVDLYSDVDDITISKEPFLYGQVVDNTGQPIRWEGRASNFADYLLK
                     NRLKSRSNGLRIIYSVTINMVPNHLDKRAHKYLGMVRQASRKYGVDESLILAIMQTES
                     SFNPYAVSRSDALGLMQVVQHTAGKDVFRSQGKSGTPSRSFLFDPASNIDTGTAYLAM
                     LNNVYLGGIDNPTSRRYAVITAYNGGAGSVLRVFSNDKIQAANIINTMTPGDVYQTLT
                     TRHPSAESRRYLYKVNTAQKSYRRR"
     gene            complement(6345..6620)
                     /gene="yggX"
                     /locus_tag="ACHKBL_003086"
     CDS             complement(6345..6620)
                     /gene="yggX"
                     /locus_tag="ACHKBL_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HFO5819923.1"
                     /translation="MSRTIFCTFLQREAEGQDFQLYPGELGKRIYNEISKEAWAQWQH
                     KQTMLINEKKLNMMNAEHRKLLEQEMVNFLFEGKEVHIEGYTPEDKK"
     gene            complement(6648..7700)
                     /gene="mutY"
                     /locus_tag="ACHKBL_003087"
     CDS             complement(6648..7700)
                     /gene="mutY"
                     /locus_tag="ACHKBL_003087"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HFO5819924.1"
                     /translation="MQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEVMLQQTQ
                     VATVIPYFERFMARFPTVTDLANAPLDEVLHLWTGLGYYARARNLHKAAQQVATLHGG
                     KFPETFEEVAALPGVGRSTAGAILSLSLGKHFPILDGNVKRVLARCYAVSGWPGKKEV
                     ENKLWSLSEQVTPAVGVERFNQAMMDLGAMICTRSKPKCSLCPLQNGCIAATNNSWSL
                     YPGKKPKQTLPERTGYFLLLQHEDEVLLAQRPPSGLWGGLYCFPQFADEESLRQWLAQ
                     RQIAADNLTQLTAFRHTFSHFHLDIVPMWLPVSSFTGCMDEGNALWYNLAQPPSVGLA
                     APVERLLQQLRTGAPV"
     gene            7861..8580
                     /gene="trmB"
                     /locus_tag="ACHKBL_003088"
     CDS             7861..8580
                     /gene="trmB"
                     /locus_tag="ACHKBL_003088"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HFO5819925.1"
                     /translation="MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQEHALENYWPV
                     MGVEFSEDMLDFPALFGREAPVTLEIGFGMGASLVAMAKDRPEQDFLGIEVHSPGVGA
                     CLASAHEEGLSNLRVMCHDAVEVLHKMIPDNSLRMVQLFFPDPWHKARHNKRRIVQVP
                     FAELVKSKLQLGGVFHMATDWEPYAEHMLEVMSSIDGYKNLSESNDYVPRPASRPVTK
                     FEQRGHRLGHGVWDLMFERVK"
     gene            8580..8906
                     /gene="yggL"
                     /locus_tag="ACHKBL_003089"
     CDS             8580..8906
                     /gene="yggL"
                     /locus_tag="ACHKBL_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HFO5819926.1"
                     /translation="MAKNRSRRLRKKMHIDEFQELGFSVAWRFPEGTSEEQIDKTVDD
                     FINEVIEPNKLAFDGSGYLAWEGLICMQEIGKCTEEHQAIVRKWLEERKLGEVRTSEL
                     FDVWWD"
     gene            9090..9809
                     /gene="yggN"
                     /locus_tag="ACHKBL_003090"
     CDS             9090..9809
                     /gene="yggN"
                     /locus_tag="ACHKBL_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2884 domain-containing protein"
                     /protein_id="HFO5819927.1"
                     /translation="MMRKMLLAAALSVTAMTAHADYQCSVTPRDDVIVSPQTVQVKGE
                     NGNLVITPDGNVMYNGKQYSLNAAQREQAKDYQAELRSTLPWIDEGAKSRVEKARIAL
                     DKIIVQEMGESSKMRSRLTKLDAQLKEQMNRIIETRSDGLTFHYKAIDQVRAEGQQLV
                     NQAMGGILQDSINEMGAKAVLKSGGNPLQNVLGSLGGLQSSIQTEWKKQEKDFQQFGK
                     DVCSRVVTLEDSRKALVGNLK"
     gene            9985..11031
                     /gene="ansB"
                     /locus_tag="ACHKBL_003091"
     CDS             9985..11031
                     /gene="ansB"
                     /locus_tag="ACHKBL_003091"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006528 - asparagine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HFO5819928.1"
                     /translation="MEFFKKTALAALVMGFSGAALALPNITILATGGTIAGGGDSATK
                     SNYTAGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQDMNDDVWLTLAKKINTDCDKT
                     DGFVITHGTDTMEETAYFLDLTVKCDKPVVMVGAMRPSTSMSADGPFNLYNAVVTAAD
                     KASANRGVLVVMNDTVLDGRDVTKTNTTDVATFKSVNYGPLGYIHNGKIDYQRTPARK
                     HTSDTPFDISKLNELPKVGIVYNYANASDLPAKALVDAGYDGIVSAGVGNGNLYKTVF
                     DTLATAAKNGTAVVRSSRVPTGATTQDAEVDDAKYGFVVSGTLNPQKARVLLQLALTQ
                     TKDPQQIQQIFNQY"
     gene            11148..12155
                     /gene="yggM"
                     /locus_tag="ACHKBL_003092"
     CDS             11148..12155
                     /gene="yggM"
                     /locus_tag="ACHKBL_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HFO5819929.1"
                     /translation="MKKQWIVGTALLMLMTGNVWADGEPPTENILKDQFKKQYHGILK
                     LDVITLKNLDAKGNQATWSAEGDVSSSDDLYTWVGQLADYELLEQTWTKDKPIKFSAM
                     LTSKGTPASGWSVNFYSFQAAASDRGRVVDDIKTNNKYLIVNSEDFNYRFSQLESALN
                     NQKNSIPALEKEVKALDKQMVAAQKAADAYWGKDANGKQMTREDAFKKIHQQRDEFNK
                     QNDSEAFAVKYDKEVYQPAIAACHKQSADCYEVPIQQKRDFDINEQRRQTFLQSQKLS
                     RKLQDDWITLEKGQYPLTMKVSEINSKKVAILMKIDDINQTNERWKKDTEQLRRNGVI
                     K"
     gene            complement(12211..13512)
                     /locus_tag="ACHKBL_003093"
     CDS             complement(12211..13512)
                     /locus_tag="ACHKBL_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708726.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HFO5819930.1"
                     /translation="MDSYIALTLFGCFFVLVFIGVPISFSIGIATVASMLLMFPWDIA
                     AITVSQRLANGLDNFALLAIPFFIFAGTLMNSGGIAIRLINLAQVMVGRVPGSLGHVN
                     VLANMMFGSISGSAVAAAAAVGGTLNPIQTKEGYDPAFSTAVNVSSCITGLLIPPSNV
                     LIVFSLTAGGVSVASLFMAGYLPGILMGLAVMIVCGIIAKRRGYPLSERATFAQACKA
                     FLDALPSLLLVFIVMGGILGGIFTATEASAIAVVYTFILSVLIYREVKWRDLPKLILE
                     SVVTTSIVLLLIGFSVGMSWAMTNADIPYMISDALMGISDNPLVILLLINIVLLIVGI
                     FMDMTPAVLIFTPIFLPIAQELGMDPVHFGIMMVANLCIGLLTPPVGSALFVGCSISG
                     VKIQHLIKPLLPFYAALLIALMMITYIPQISLFIPQLLGLM"
     gene            complement(13512..14015)
                     /locus_tag="ACHKBL_003094"
     CDS             complement(13512..14015)
                     /locus_tag="ACHKBL_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HFO5819931.1"
                     /translation="MIFNRLKLAVDRVIAAFSVAVMLALVVCVVWQVFSRYVLNQPST
                     LTDELARFLMIWVGLLGAAYTVGAQRHLSIDLFALALNKRKQLLLSIVINVLILGFAG
                     SVIVTGGLKLIDKTLATSQVSAAMQIPMGYVYIILPLSGLVMMFYALCFINQSIQQLK
                     QPAQEVS"
     gene            complement(14060..15046)
                     /locus_tag="ACHKBL_003095"
                     /pseudo
     CDS             complement(14060..15046)
                     /locus_tag="ACHKBL_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231167.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
BASE COUNT         4033 a         3646 c         3857 g         3685 t
ORIGIN      
        1 cagacactgg aagatgccct gagtttgtat acagatgagc gcacagtgag caagatttgc
       61 aaagggggga ataaatcttg acaggctgaa tcagcaatgt aagcatacag tgttaacgag
      121 aacagggcgt ctctgcaaaa gacaccctgt ttgaggatac gataccgaac tcaatcagtg
      181 taaccacaac ttacacaaac ggagaccctt tgatgccgca tcctgtatta accgcccgtc
      241 aaggatctca tcccttaacc agcgtgttct ggtatatggt gcccggaggc ggaatcgaac
      301 caccgacacg gggattttca atcccctgct ctaccgactg agctatccgg gcaacggggc
      361 gcattaaacc tgattcgcct cgtctcgtca atcaaatttc ttcaatttac tgcagactgc
      421 acaatctatc atcactttgc cgctattgca cgcattgtca ggcaaaccag gcggtccagg
      481 cggcggaaag cggcattgcc agaaggagtg ctggaagcat gttgaccacc gggaacattt
      541 taatgccaca gatgcgtaat ccggttgcca gcagcaataa accgcctacg gcgctgaagt
      601 ctgccatcat cgacggtgtg gtcagcggta atatcagcgc ggcagcccac gccagcgtta
      661 actggatgat cagtaatggg atactaatta ccgacaccgc aatacccaac gagcaggcga
      721 agatcatcgc cgtaaagaaa tcgagaaatg acttggcgat taaaatactc ggatcgccgg
      781 tcatcccttc gttcatcgcc ccgaagatcc cggtgccgct ggcgcaaaac aggacaataa
      841 tcgcgacata attctgaata aaagattcat gcgctggctt cttacgtgag tggcgaaaca
      901 gattttgtgc tttggtgacc gcggtattga cgcctttttc cagcagacaa atttcgccga
      961 ttaatgcacc gagtaaggtt gctaaaacca tcgccggaag gttggcgcat ttcaccacca
     1021 gtaaaatacc aatccccagc gatgccagac caaaaataga tgtcatggag acgcggatac
     1081 gttccggtaa acgttggctg agcagtgcgc cgagaacgcc acccagtaag actgcactag
     1141 cgttgataaa agggccgatg accacaagag ttcctgttaa ctgtattgtc tgattttctt
     1201 attatgaact ttttgccttg ttgatggctt gtctggtcgc aaagtcctgg cttgcacgct
     1261 ttagcgaaag gtgccatgat tgcgcgaatt ttctcctctc tgtacggagt ttgcccgatg
     1321 cacgccacct ccttacattc tctcgcttat cgccgtttcg cgcgaaactc ctcccttttt
     1381 ctgctcatgc ggtgaagtta acgcacgctc actgcaggac aacagtaaaa tcagagcgtt
     1441 tctgctttta ctgatgtctg gcggtcggag ctggtgacca gtttgaccca catctcatgg
     1501 ggcaggtttg tccatcctgt ccggtactct tacttccccg aaacgggttt tgcgcttatg
     1561 aaatcaatga atattgccgc cagtagtgaa ctggtatccc gactttctac tcatcgccgc
     1621 gtggtggcgt tgggagatac tgattttacg gacgtcgcgg cagtcgtcat taccgctgcg
     1681 gatagtcgca gtggcattct tgcgttgctt aagcgcaccg gttttcatct accggtgttt
     1741 ttgtattccg aacatgttgt tgaattacct gcgggcgtta cggcggtaat caacggcaac
     1801 gagcagcagt ggctggagct ggaatccgca gcctgtcagt atgaagagaa tttgctgcca
     1861 ccgttttatg acacgctgac gcagtacgtt gagatgggca acagcacctt tgcttgccct
     1921 ggacatcaac atggtgcgtt ttttaaaaag catcctgccg gacgccattt ttacgatttc
     1981 tttggtgaga acgtctttcg cgccgatatg tgtaacgctg acgtaaaatt gggcgatctg
     2041 cttattcatg aaggttcggc gaaggatgca cagaaattcg cagccaaagt ctttcatgcc
     2101 gataaaacct attttgtgct gaacggcaca tcggcagcga ataaagtggt gacgaatgcg
     2161 ctgttaacgc gtggcgatct ggtgctcttc gaccgtaaca accataagtc gaatcatcat
     2221 ggcgcgttga ttcaggcggg ggcgacgccg gtctatctgg aagcttcacg caacccgttt
     2281 ggtttcattg gtggtattga tgcgcactgt tttaatgaag agtatctgcg ccagcaaatt
     2341 cgcgacgttg cgccagaaaa agcagacctg ccgcgtccgt ttcgtctggc gattattcag
     2401 ctgggaacct atgacggcac tgtctataac gcccgtcagg tgatcgatac cgttgggcat
     2461 ctgtgtgatt acattctgtt tgattccgcg tgggtcggtt acgaacagtt tatcccgatg
     2521 atggcggata gctcgccgct gctgttagaa cttaacgaaa acgatccggg gatctttgtg
     2581 acgcaatcgg tgcataaaca gcaggcggga ttctcacaga cgtcgcagat ccataaaaaa
     2641 gataaccata ttcgcgggca ggcgcgtttt tgcccgcata agcggttaaa taatgccttt
     2701 atgctccatg cttctaccag cccgttctat ccgctgtttg ccgcgctgga cgttaacgcc
     2761 aaaattcatg aaggggagag tgggcgtcgg ctgtgggcgg agtgcgttgc gttggggatt
     2821 gaagcccgga aggcgattct tgcgcgctgt aagctgttcc gcccgtttat tccacccgtt
     2881 gttgatggca aattgtggca ggattatccg acgtcagtgt tagccagcga ccgtcgtttt
     2941 ttcagttttg agccgggggc gaagtggcac ggctttgaag gatatgccgc ggagcagtat
     3001 tttgtcgatc cgtgcaagct gttactgacc acaccaggta ttgatgctga aaccggtgaa
     3061 tatagcgact ttggcgttcc ggcgacgatt ctggcgcatt atctgcgtga gaacggtatt
     3121 gtgccggaga agtgcgatct caactccatt ctgttcttat taacgccggc ggaaagccac
     3181 gagaagctgg cacaactggt ggcgatgctg gcgcagtttg aacagcatat tgaggatgac
     3241 tcgccgctgg cggaggtgtt gccgagcgtt tataacaaat atccggtgcg ctatcgcgac
     3301 tacactttgc gccagttgtg tcaggagatg cacgatctgt atgtcagttt cgacgtcaaa
     3361 gacctacaaa aagcgatgtt ccgccagcag agtttcccgt cagtggtgat gaacccccag
     3421 gatgcgcata gcgcttatat tcgcggtgaa gtggagttgg tgcggattcg tgatgccgaa
     3481 gggcgaattg cggcagaagg ggcgttgcct tatccaccag gcgtgctttg cgtggtaccc
     3541 ggagaagtct ggggtggggc ggttcagcgt tatttccttg cgctggaaga aggggtgaat
     3601 ttattgccgg gattttcgcc ggagctgcaa ggtgtttata gcgaaaccga tgcggatggc
     3661 atgaaacggt tgttcggtta tgtgttgaag taagaataaa aaaaacgggt caccttctgg
     3721 cgacccgttt ttctttgcgt aattagtggc taaccgtctg tgtgcctgtc gggacacgaa
     3781 cgtgtttata tttgaacatc gccatgaacg cgaaggccag aaccacggag taaccagcga
     3841 aaatcagcca tacggtctgc cagtcggtaa tgccgttttg ggtgtacatc tcaacaactt
     3901 taccgctcac gatgccgccg aggatacagc cgaagccgtt agtcatcatc aggaacatcc
     3961 cctgcgcact ggcgcgaatt gccgggctaa cttctttttc gacaaacacc gaaccagaga
     4021 tgttgaagaa gtcgaatgcg caaccgtaaa cgatcatcga cagtaccagc agtacagtac
     4081 cgaacggagt cgggtcgccg taagcaaaca gcgcaaaacg caggatccac gccacaatac
     4141 tgatcatcat tacgttctta ataccgtagc ggcttaagaa gaacgggatg gtcagaatga
     4201 acagggtttc agagatctgc gaaatcgaca tgatgattga cgcatgctgc acgatgaagc
     4261 tgctggcaaa catcggatct ttgtcgaagc tgtgcaggaa ggtgttaccg aacatgttgg
     4321 taatctgcag ttccgcgccc agcagcattg agaagatgaa gaagattgcc atacgcttgt
     4381 ttttaaacag cgcgaatgca tcgaggccca gcagggttgt ccagctctga ttcgcttgct
     4441 gtttagcaac cggaatatgc ggcagagtca gggtaaacag aaccagaatg gcggaaagtg
     4501 ctgcgccaat atacagctgc atgtggctta attcgaagcc agacaggctc accacccaca
     4561 ttgccatgat aaagccgatg gtgccccaga tacggattgg cgggaagtca gtaacgatat
     4621 ccatcccggc attttgcagg cgatagtaag agatggtgtt gattaaccca agcgttggca
     4681 tataagcaaa cgagttaatc aatatcacaa ggaacatcgc ttccggcgtc gtgacctgtg
     4741 ccgccatgaa cagcgtgata gcgccaatgg tgtggcaaat ggcatatacc catttcgcac
     4801 ttaaccattt gtcggccaca atccccagca gcgcaggcat aaataccgcg gcgataccca
     4861 gtgagctata aactgcgcca atagaagcac cgtcaaactt cagggtaaca aacatatagg
     4921 agccgagggt cgtcagccaa cttccccaca gacagaactg cagaaaagag aggattttca
     4981 gctgcagctt aagattcatg ttaatttcct cacatcgtga tgcggatgag tgtttgaaaa
     5041 agcacatttg accgtgcgag atgatgggat tctatcaact tcccggactg gttttttgtt
     5101 acctgtggca aataattgca aacattccca atttgcaaaa cgaaattgtg atgtggataa
     5161 catttttacc cctgagcatc gtcaggggcg gttagtggaa ttatcggcgg cggtaggatt
     5221 tttgcgcggt attcacttta taaagataac ggcgagattc cgcagaggga tggcgggtcg
     5281 tcagcgtttg ataaacatcg cccggcgtca tggtgttaat aatattggcc gcctgaatct
     5341 tatcattcga aaagactcgc agcacgctgc ctgcgccgcc gttataggcg gtgatgacgg
     5401 cataacgccg ggatgtaggg ttatcaattc cgccgagata aacgttgttc agcatcgcca
     5461 gatacgcggt gccggtatca atattgctgg caggatcaaa caagaaactg cggctcggcg
     5521 tgccggattt cccctgcgaa cggaacacat ctttcccggc agtatgttgt accacctgca
     5581 ttaaccccag cgcatcggaa cggctgaccg catacgggtt aaaggatgac tcggtctgca
     5641 taatcgccag aatcagcgac tcatcaacgc catatttccg tgacgcctgg cggaccatgc
     5701 cgagatattt gtgcgcacgt ttatcaaggt ggttcggcac catgttaatg gtgacgctgt
     5761 agatgatacg cagcccgttg ctgcggctct tcagacggtt tttcagcaga taatccgcga
     5821 agttgctcgc gcgaccttcc cagcgaatcg gctgcccggt gttgtccacc acctgaccgt
     5881 aaaggaaagg ttctttcgaa atcgtaatat catcaacgtc ggaatagaga tcgaccgaac
     5941 tcggatcgtc ccccatcagc aacgttttga taattgcccg gcgcagatgc gcggcaggtt
     6001 ctgtcccggc gatggtttcg atagtaatcg taccgtcatc gaagttgatg tggctgcggg
     6061 tctgatattg atcggtgtat ttcacgtagt ccttaggacc ggcgatcacc acctctttga
     6121 agccccagat gttctcaata ttgtgggcaa attgccccat cagaatatca aaaccgttgg
     6181 tatctttgac ccaggcttcg ttataggtat cgcctttttt ggtcgtcgaa caggagatga
     6241 gcaacggcgc aatcaaagcc agcgcgagat attttttcat cattccgggt gcgtgttgtg
     6301 tttgttttta tgcaagttgc cggaggcgtg ctccggcact gtttttattt tttatcttcc
     6361 ggcgtatagc cctcaatatg cacctcttta ccctcgaaca ggaagttgac catctcctgt
     6421 tccagcagct tgcggtgctc ggcattcatc atgttgagtt tcttttcatt aatcagcatg
     6481 gtttgtttgt gctgccactg cgcccaggct tctttggaga tctcgttata gatgcgtttt
     6541 cccagctcgc cggggtacag ctgaaaatcc tgaccttctg cttcacgttg caggaaagta
     6601 caaaaaatcg ttctgctcat aaatcatcct ctttatcgac tcacgcgcta aaccggcgcg
     6661 ccagtgcgta actgctgtaa caaacgctcc acgggagccg ccaggccaac tgacggcggt
     6721 tgcgctaagt tataccagag cgcattgcct tcatccatgc agccggtgaa tgacgacacg
     6781 ggaagccaca taggcacaat atctaagtgg aaatggctga aggtatgccg aaacgcggtc
     6841 agctgcgtca ggttatcggc agcaatctgc cgttgcgcca gccactgccg caaactttct
     6901 tcgtcggcaa actgcgggaa acagtataaa ccgccccaca atccgctcgg cggacgctgc
     6961 gccagcaata cttcatcttc gtgctgtaac aggagaaagt agccggtgcg ctccggcagc
     7021 gtctgtttcg gttttttgcc cggataaagc gaccagctat tgttggtggc ggcaatacat
     7081 ccgttttgta gcggacagag cgagcatttc ggcttcgagc gcgtgcaaat catcgcaccc
     7141 aaatccatca tcgcctgatt aaaccgctcc acgccgaccg cgggcgtcac ctgttcgctc
     7201 aaactccaca gtttattctc gacctctttt ttcccaggcc agccgcttac agcatagcag
     7261 cgcgccagca cccgtttgac gttaccgtcg agaatcggaa agtgcttacc cagagaaagc
     7321 gagagaattg cgcctgcggt ggaacgcccg acgcccggta acgccgcgac ttcttcaaag
     7381 gtttccggga atttaccgcc gtgtaaggtc gccacttgtt gtgccgcctt atgcagattg
     7441 cgcgcgcggg cgtaatagcc aagcccggtc cacaagtgga gaacttcgtc gagcggcgca
     7501 ttggcgagat cggtcaccgt cgggaagcgc gccataaagc gttcaaaata ggggataacg
     7561 gtcgcaacct gagtttgttg caacatcact tctgagagcc atactttgta gggcgtcttg
     7621 tcaatttgcc agggcagcgt tttccgcccg tatttatcgt accagtccag aacctgggct
     7681 gaaaattgcg acgcttgcat ggtcaccgaa ttcactgttg ttgggggcaa gattgcagca
     7741 cagcgacggc agggtgtaaa ccggaacttt ccgcagccac ggccttatca tgcttgcatc
     7801 cggcaactaa ctttggataa tgcccgtttt cagaacactt tcacaagcga ctaaatcttt
     7861 atgaaaaacg acgtcatctc accggaattt gatgaaaacg gccgcccact gcgccgtatc
     7921 cgtagttttg tgcgccgcca ggggcgactg accaaaggcc aggaacatgc gctggaaaac
     7981 tactggccgg tgatgggcgt tgagttcagc gaagatatgc tggatttccc cgcgcttttt
     8041 ggccgagaag cgccggtgac gcttgagatt ggtttcggca tgggggcgtc gctggtggca
     8101 atggctaaag atcgccccga gcaggacttc ctcggcattg aagtgcattc accgggcgtt
     8161 ggtgcgtgcc tggcttctgc gcatgaagag ggcttaagca acctgcgcgt gatgtgtcac
     8221 gatgcggttg aagtgctgca taaaatgatt cctgacaatt cattgcgcat ggtgcagctc
     8281 tttttccctg acccgtggca caaagcgcgc cataataaac gccgtatcgt tcaggtgccg
     8341 tttgccgaac tggtaaaaag caaactgcaa ctggggggcg tattccatat ggcgaccgac
     8401 tgggaacctt atgcggaaca tatgcttgaa gtgatgtctt ctattgacgg ttataaaaac
     8461 ctgtcagaga gcaatgatta cgtaccgcgt ccggcatcac gtccggtgac gaaatttgaa
     8521 caacgtggtc atcgtcttgg tcacggagta tgggacttaa tgttcgagag ggtgaaataa
     8581 tggcaaagaa ccgtagccgt cgtctgcgta aaaaaatgca catcgacgaa ttccaggaat
     8641 taggattttc ggtggcatgg cgattcccgg aaggtacatc ggaagaacag attgataaaa
     8701 ccgttgatga ctttattaac gaggttatcg aaccgaacaa actggccttt gacggcagcg
     8761 gttatctggc ctgggaaggt ctgatctgca tgcaggaaat cggcaaatgc accgaagaac
     8821 atcaggcgat tgtgcgtaag tggctggaag agcgcaaact gggcgaggta cgcaccagcg
     8881 aacttttcga cgtttggtgg gactaagaaa gcatacgggc gatgacaaat gcaaaactgc
     8941 ctgatgtgct acgcttatca ggcctataaa gatgcacgat cgagtaggct ggataaggcg
     9001 tttacgccgc atccggcatg gaaaacgcgc actttgttat caatctgggg ccagcaaatg
     9061 ctggcctgat ttgttcttga gggaagacta tgatgcgcaa aatgctgctg gcggcagcac
     9121 tttcagtgac ggcaatgacc gctcacgccg actaccagtg cagcgtcacg ccgcgtgacg
     9181 atgtgattgt cagcccgcaa accgtgcagg tgaagggcga aaacggcaat ctggtgatca
     9241 cgccagacgg caacgtgatg tataacggta agcaatattc cctgaatgcc gcccagcgcg
     9301 agcaggcgaa ggattatcag gctgaactcc gtagcaccct gccgtggatt gatgaaggcg
     9361 cgaaaagccg cgtcgaaaaa gcccgtattg cgctggataa aattatcgtt caggagatgg
     9421 gcgaaagcag caaaatgcgc agccgtctga ccaaacttga tgcgcagctg aaagagcaga
     9481 tgaaccgcat tatcgaaacg cgcagcgatg gcctgacgtt tcactataaa gccattgatc
     9541 aggttcgcgc cgaaggccag caattagtga atcaggcaat gggtggaatt ttacaggaca
     9601 gcattaatga aatgggcgcg aaagcggtgc tgaaaagcgg cggtaaccca ttacagaatg
     9661 tgctgggaag cctgggcggt ctgcaatcct caatccaaac cgagtggaaa aagcaggaaa
     9721 aagatttcca gcagtttggc aaagatgttt gtagccgcgt tgtgactctg gaagatagcc
     9781 gcaaagccct ggtcgggaat ttaaaataat cctctatttt aagacggcat aatacttttt
     9841 tatgccgttt aattcttcgt tttgttacct gcctctaact ttgtagatct ccaaaatata
     9901 ttcacgttgt aaattgttta acgtcaaatt tcccatacaa agctaaggga taatgcgtag
     9961 cgttcacgta actggaggaa tgaaatggag tttttcaaaa agacggcact tgccgcactg
    10021 gttatgggtt ttagtggtgc agcattggca ttacccaata tcaccatttt agcaactggc
    10081 gggaccattg ccggtggtgg tgactccgca accaaatcta actacacagc gggtaaagtt
    10141 ggcgtagaaa atctggttaa tgcggtgccg caactgaagg acattgcgaa cgttaaaggc
    10201 gagcaggtag tgaatattgg ctcccaggac atgaacgatg atgtctggct gacactggcg
    10261 aaaaaaatta acaccgactg cgataaaacc gacggcttcg tcattaccca cggtaccgac
    10321 acgatggaag aaaccgctta cttcctcgac ctgacggtga aatgcgacaa accggtggtg
    10381 atggtcggcg caatgcgccc gtccacgtcc atgagcgcag acggtccatt caacctgtat
    10441 aacgcggtag tgaccgcagc tgataaagcc tccgctaatc gtggcgtgct ggtggtaatg
    10501 aacgacaccg tactggacgg tcgcgatgtc accaaaacca ataccaccga cgtagcgacc
    10561 ttcaagtctg ttaactacgg tcctctggga tacattcaca acggtaagat tgactaccaa
    10621 cgtaccccgg cacgtaagca caccagcgat acgccgttcg atatctctaa gctgaatgag
    10681 ctgccgaaag tcggcatcgt ttataactac gctaacgcat ccgatcttcc ggctaaagca
    10741 ctggtagatg cgggctatga tggcattgtt agcgctggtg tgggtaacgg caacctgtat
    10801 aaaaccgtat ttgacactct ggcgaccgcc gcgaaaaacg gcactgcagt cgtgcgttct
    10861 tcccgcgtac cgacgggcgc taccactcag gatgccgaag tggatgatgc gaaatatggc
    10921 ttcgtggtct ctggcacgct gaacccgcaa aaagcgcgcg ttctgctgca actggctctg
    10981 acgcaaacca aagatccgca gcagatccag cagatcttca atcagtacta atcacctcgc
    11041 cccggtaacg tgccggggct ttttcacttc agacttacgt ccattgccaa ttttcattac
    11101 cctgatgata atcactggaa taaattattc cgcgcgaggg ttttcgggtg aaaaagcaat
    11161 ggattgttgg cacggcgctg cttatgttga tgactggtaa tgtctgggca gatggcgaac
    11221 cgccaactga aaatatctta aaagatcaat tcaaaaagca gtatcacggc attctcaagc
    11281 ttgatgtcat caccttaaaa aatcttgatg ctaagggtaa tcaggccacc tggtcagcgg
    11341 aaggcgatgt ctcttccagt gacgatctct atacctgggt cggtcagttg gcagattacg
    11401 aactgctcga acagacctgg acgaaagata aaccgataaa attctcggcg atgttaacca
    11461 gtaaaggaac gcccgcgtct ggctggtcgg tgaactttta ctcttttcag gcggcagcca
    11521 gcgatcgtgg acgggtggtc gacgatatca aaacgaataa taaatatctg atcgtgaata
    11581 gcgaagattt caattatcgc tttagtcagc ttgaatcagc gttgaataac cagaagaact
    11641 cgattcctgc gctggaaaag gaagtgaaag cgcttgataa gcaaatggtg gcggcgcaga
    11701 aagcagcgga tgcatattgg ggaaaagatg caaacggtaa gcaaatgacc cgcgaagacg
    11761 cctttaaaaa aattcaccaa cagcgtgatg agtttaataa acagaacgat agcgaggcgt
    11821 tcgcggttaa atatgacaaa gaggtttatc aaccggcgat tgcggcatgt cataaacaga
    11881 gtgcagattg ttatgaagtg ccgattcagc agaagcgaga tttcgatatc aacgaacaac
    11941 ggcgacagac ttttctgcaa tcacaaaaac ttagccgtaa attgcaggat gactggataa
    12001 cgctcgaaaa agggcaatat ccgttaacga tgaaagtttc agaaataaac agtaaaaaag
    12061 tcgcgatttt gatgaaaatt gatgatatca accagactaa tgaacgctgg aaaaaagata
    12121 ccgaacaatt gcggcgcaat ggagtgatta agtaactcat gttaagtacc tttgccggat
    12181 aatataaaaa gttatccggc aaattatttt ttacattagc cctagtagct gtggaatgaa
    12241 caaggatatt tgcgggatgt aagtgatcat catcaacgcg attaacaatg cggcataaaa
    12301 tggcagcaat ggtttgatca aatgttgaat cttcacacct gaaatagaac aaccaacaaa
    12361 tagcgcacta cccaccggcg gtgttaacag cccaatacaa aggttagcca ccatcataat
    12421 accgaaatgt accggatcca ttcccagctc ttgtgcaatc ggcaagaaga ttggtgtgaa
    12481 gattagtaca gccggtgtca tgtccataaa gatgccgaca attaataaga cgatattaat
    12541 cagcagcaat atgaccagtg ggttatcaga aatccccatc aacgcgtcgc tgatcatata
    12601 aggaatatca gcattggtca ttgcccagga catgcccacg gaaaaaccga tcaacagtaa
    12661 tacaatcgaa gtggtaacaa ccgattccag aatcagtttg ggtaaatcgc gccattttac
    12721 ttcccggtaa atcagcaccg agagaataaa tgtgtagaca acggcaattg ctgacgcttc
    12781 agttgcggta aaaatgccgc ccagaatgcc gcccattaca atgaacacca gaagcaaact
    12841 tggtaaggca tccagaaacg ctttacatgc ctgagcaaag gttgctcttt ctgaaagtgg
    12901 gtacccacgg cgtttagcaa taatgccgca gactatcatt accgcgagac ccattaagat
    12961 ccctggcaaa tatcccgcca taaatagcga tgctacagaa acgccgccag ctgtcaggga
    13021 aaacacaatt aacacgtttg agggcggtat cagtaatcca gtaatacaag atgagacgtt
    13081 tacagcggta gaaaatgccg gatcataacc ttctttggtc tgaatgggat tcagtgtgcc
    13141 accaacggca gcagctgccg caacggctga accagaaatg gaaccaaaca tcatattcgc
    13201 caggacgttc acatgaccaa gcgaaccagg aacgcggcca accattacct gggcgaggtt
    13261 aatcaaacgt atcgcaatac cgccgctgtt catcagcgtt ccggcaaaga tgaaaaaggg
    13321 gattgccaat agggcaaagt tatccagtcc attagccagt cgctgcgaaa cagtgattgc
    13381 cgcgatatcc cagggaaaca tcaacagcat tgaagctaca gtggcaatac cgattgagaa
    13441 cgaaatgggc acaccaataa agaccagcac aaaaaaacag ccaaacagtg taagcgcgat
    13501 ataactatcc attaactgac ctcctgcgca ggttgtttca gttgttgaat gctttgatta
    13561 ataaaacaca aggcataaaa catcatcacc aatccactta atggcagaat gatatatacg
    13621 tatcccatcg gtatttgcat cgcggcagat acctgtgatg ttgccagtgt tttatcaatt
    13681 aatttcaggc caccagtcac aatgaccgaa ccggcaaaac ctaaaataag aacgttaatg
    13741 actatactaa gaagtaattg cttgcgctta ttcagggcga gagcaaataa atcaatagag
    13801 agatgtctct gcgcgccgac ggtatatgca gcacctaata atcctaccca gatcatcaaa
    13861 aagcgtgcca gctcatcggt taatgtactt ggctggttaa gtacgtagcg gctaaatact
    13921 tgccagacta cgcatacaac cagagcaagc attacggcga cagaaaacgc ggcaatgacg
    13981 cggtctaccg caagtttcag gcggttaaat atcattgtaa gcatcctgga aaatcagggc
    14041 cagagactgg cccgaaatat tattgagcag cgttgtcgaa tttctctaac aaagcagctt
    14101 gttttggatc ttttttgaag tcgtcaaaca gaggctgaac tgcggcacgg aatggggctt
    14161 tatcaacctt aacgatttca ccacccatgg ctttcgcctg agcacgtgtg tcagcgtcaa
    14221 ttttttccca caatttttgt tgataagctt cggatttctt cgccgctgtt tcgaggattt
    14281 gttgctgttc tggggttaat ttattccatg ttttggtgga gataaccaaa aagtcaggaa
    14341 ttgaggcatg ttcatcttca gaaaagactt tggcaatttc gatatgtcga gtctgcaccc
    14401 aggaaggcac gttattttct gcgccatcta ctacaccttg ctgcatcgca gtataaactt
    14461 cgccaaatga aattggagtg ggtgagccac ccatcagttc aatcattttg atggtggtcg
    14521 ggcttggctg aacacgaatt ttcagccctt taagatcttc tggcttagta atcggttttt
    14581 tcgcatagaa actgcgtgta cccgctacat aagctgataa agcaaaaaag cctttatctt
    14641 ttgttgaatc cataatttct ttaccaactt caccaaaaac cactttgttg aagtgcgcct
    14701 gatctttaaa taagaacggc aaattataga tagcataaat attatcaaaa gactccagat
    14761 cactggcgga acctttggtc atatccagtg caccattttg caacaactac atagtttctc
    14821 gcgcattacc catctggctg ctggggtaaa tacggatcac catattacct tttgacagct
    14881 gttttacttc tttcgccagc tcttcaaaac tctgatgtac aacatgactt ctttccaggt
    14941 tgtgtgctaa ctttaacgtc actttttctg cggcgtttac gccagtgcta accagagcca
    15001 gcaaggatgc gcagactaat gagcgggtaa atgacctcat tttcataaaa aaactccctg
    15061 ttgaagaatg gaaattggtt gatatctaat tgtgcttatc agtgctttgt gcgacaaatt
    15121 ggttaacctg tctgacaact tgtctatgtt tatcacaact tgaaataaag tcagaaatta
    15181 aatattaatt ttgtgcgcca ggttgtacat gtttatactc a
//
LOCUS       DAZFLD010000119        15199 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.120, whole genome shotgun sequence.
ACCESSION   DAZFLD010000119 DAZFLD010000000
VERSION     DAZFLD010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15199
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.120"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..500)
                     /gene="curA"
                     /locus_tag="ACHKBL_003096"
     CDS             complement(<1..500)
                     /gene="curA"
                     /locus_tag="ACHKBL_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent curcumin/dihydrocurcumin
                     reductase"
                     /protein_id="HFO5819932.1"
                     /translation="MGQQKQRNRRWVLASRPHGAPVPENFRLEEDDIATPGEGQVLLR
                     TVYLSLDPYMRGRMSDEPSYSPPVDIGGVMVGGTVSRVVESNHPDYQPGDWVLGYSGW
                     QDYDISSGDDLVKLGDHPQNPSWSLGVLGMPGFTAYMGLLDIGQPKEGETLVVAAATG
                     PVGATVG"
     gene            681..1196
                     /gene="mddA"
                     /locus_tag="ACHKBL_003097"
     CDS             681..1196
                     /gene="mddA"
                     /locus_tag="ACHKBL_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine sulfoximine/L-methionine sulfone
                     acetyltransferase"
                     /protein_id="HFO5819933.1"
                     /translation="MSIRFARKADCAAIAEIYNHAVLYTAAIWNDQTVDADNRIAWFE
                     ARTIAGYPVLVSEEDGVVTGYASFGDWRSFDGFRHTVEHSVYVHPDHQGKGLGRKLLS
                     RLIDEARDCGKHVMVAGIESQNQASLHLHQSLGFVVTAQMPQVGTKFGRWLDLTFMQL
                     QLDERSEPDAI"
     gene            1196..1645
                     /gene="ydcZ"
                     /locus_tag="ACHKBL_003098"
     CDS             1196..1645
                     /gene="ydcZ"
                     /locus_tag="ACHKBL_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFO5819934.1"
                     /translation="MNQSLTLAFLIAAGIGLVVQNTLMVRITQTSSTILIAMLLNSLV
                     GIVLFVSILWFKQGMAGFGELVSNVRWWTLIPGLLGSFFVFASISGYQNVGAATTIAV
                     LVASQLIGGLVLDIFRSHGVPLRALIGPICGAILLVVGAWLVARRTF"
     gene            complement(1646..1879)
                     /gene="ydcY"
                     /locus_tag="ACHKBL_003099"
     CDS             complement(1646..1879)
                     /gene="ydcY"
                     /locus_tag="ACHKBL_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2526 family protein YdcY"
                     /protein_id="HFO5819935.1"
                     /translation="MSHLDEVIARVDAAIEESVIAHMNELLIALSDDAELSREERYTQ
                     QQRLRTAIAHHGRKHKEDMEARHEQLTKGGTIL"
     gene            complement(1965..2138)
                     /gene="ortT"
                     /locus_tag="ACHKBL_003100"
     CDS             complement(1965..2138)
                     /gene="ortT"
                     /locus_tag="ACHKBL_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orphan toxin OrtT"
                     /protein_id="HFO5819936.1"
                     /translation="MSLYQHMLVFYAVMAAIAFLITWFLSHDKKRIRFLSAFLVGATW
                     PMSFPVALLFSLF"
     gene            2333..2428
                     /gene="yncL"
                     /locus_tag="ACHKBL_003101"
     CDS             2333..2428
                     /gene="yncL"
                     /locus_tag="ACHKBL_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response membrane protein YncL"
                     /protein_id="HFO5819937.1"
                     /translation="MNVSSRTVVLINVFAAVGLFTLISMRFGWFI"
     gene            complement(2525..3949)
                     /gene="patD"
                     /locus_tag="ACHKBL_003102"
     CDS             complement(2525..3949)
                     /gene="patD"
                     /locus_tag="ACHKBL_003102"
                     /EC_number="1.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019145 - aminobutyraldehyde dehydrogenase
                     (NAD+) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009447 - putrescine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminobutyraldehyde dehydrogenase"
                     /protein_id="HFO5819938.1"
                     /translation="MQHKLLINGELVSGEGEKQPVYNPATGDVLLEIAEASAEQVDAA
                     VRAADAAFAEWGQTTPKVRAECLLKLADVIEENGQVFAELESRNCGKPLHSAFNDEIP
                     AIVDVFRFFAGAARCLNGLAAGEYLEGHTSMIRRDPLGGVASIAPWNYPLMMAAWKLA
                     PALAAGNCVVLKPSEITPLTALKLAELAKDIFPAGVINVLFGRGKTVGDPLTGHPKVR
                     MVSLTGSIATGEHIISHTAPSIKRTHMELGGKAPVIVFDDADIEAVVEGVRTFGYYNA
                     GQDCTAACRIYAQKGIYDTLVEKLGAAVATLKSGAPDDESTELGPLSSLAHLERVSKA
                     VEEAKATGHIKVITGGEKRKGNGYYYAPTLLAGALQDDAIVQKEVFGPVVSVTLFDNE
                     EQVVNWANDSQYGLASSVWTKDVGRAHRVSARLQYGCTWVNTHFMLVSEMPHGGQKLS
                     GYGKDMSLYGLEDYTVVRHVMVKH"
     gene            complement(3971..4765)
                     /gene="ydcV"
                     /locus_tag="ACHKBL_003103"
     CDS             complement(3971..4765)
                     /gene="ydcV"
                     /locus_tag="ACHKBL_003103"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO5819939.1"
                     /translation="MHSERAPFFLKLAAWGGVVFLHFPILIIAVYAFNTEDAAFSFPP
                     QGLTLRWFSVAAQRSDILDAVTLSLKVAALATLIALVLGTLAAAALWRRDFFGKNAIS
                     LLLLLPIALPGIVTGLALLTAFKTINLEPGFFTIVVGHATFCVVVVFNNVIARFRRTS
                     WSLVEASMDLGANGWQTFRYVVLPNLSSALLAGGMLAFALSFDEIIVTTFTAGHERTL
                     PLWLLNQLGRPRDVPVTNVVALLVMLVTTLPILGAWWLTREGDNGQ"
     gene            complement(4755..5696)
                     /gene="ydcU"
                     /locus_tag="ACHKBL_003104"
     CDS             complement(4755..5696)
                     /gene="ydcU"
                     /locus_tag="ACHKBL_003104"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO5819940.1"
                     /translation="MAMNVLQSPSRPGLGKVSGFFWGNPGLGLFLLLLGPLMWFGIVY
                     FGSLLTLLWQGFYTFDDFTMSVTPELTLANIRALFNPANYDIILRTLIMAVAVTIASA
                     ILAFPMAWYMARYTSGKMKAFFYIAVMLPMWASYIVKAYAWTLLLAKDGVAQWFLQHL
                     GLEPLLTAFLTLPAVGGNTLSTSGLGRFLVFLYIWLPFMILPVQAALERLPPSLLQAS
                     ADLGARPRQTFRYVVLPLAIPGIAAGSIFTFSLTLGDFIVPQLVGPPGYFIGNMVYSQ
                     QGAIGNMPMAAAFTLVPIVLIALYLAFVKRLGAFDAL"
     gene            complement(5697..6710)
                     /gene="ydcT"
                     /locus_tag="ACHKBL_003105"
     CDS             complement(5697..6710)
                     /gene="ydcT"
                     /locus_tag="ACHKBL_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO5819941.1"
                     /translation="MTYAVEFDNVSRLYGDVRAVDGVSIAIKDGEFFSMLGPSGSGKT
                     TCLRLIAGFEQLSGGAISIFGKSASNLPPWERDVNTVFQDYALFPHMSILDNVAYGLM
                     VKGVDKKQRYAMAQEALEKVALGFVQQRKPSQLSGGQRQRVAIARALVNEPRVLLLDE
                     PLGALDLKLREQMQLELKKLQQSLGITFIFVTHDQGEALSMSDRVAVFNNGRIEQVDS
                     PRDLYMRPRTPFVAGFVGTSNVFDALMADKLCGMTGSFALRPEHIRFNIPGEVQANGT
                     IQAVQYQGAATRFELRLNGGEKLLVSQANMTGEELPATLTPGQQVMVSWSRDVMVPLV
                     EER"
     gene            complement(6728..7873)
                     /gene="ydcS"
                     /locus_tag="ACHKBL_003106"
     CDS             complement(6728..7873)
                     /gene="ydcS"
                     /locus_tag="ACHKBL_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter substrate-binding
                     protein YdcS"
                     /protein_id="HFO5819942.1"
                     /translation="MSKTFARSSLCALTMTIMTAHAAEPPTNLDKPEGRLDIIAWPGY
                     IERGQTDKQYDWVTQFEKETGCAVNVKTAATSDEMVSLMAKGGYDLVTASGDASLRLI
                     MGKRVQPINTALIPNWKALDPRVVKGDWFNVGGKVYGTPYQWGPNLLMYNTKTFPTPP
                     DSWQVVFVEQNLPDGKSNKGRVQAYDGPIYIADAALFVKATQPQLGIRDPYQLTEEQY
                     QAVLKVMRDQHSLIHRYWHDTTVQMSDFKNEGVVASSAWPYQANALKAEGQPVATVFP
                     KEGVTGWADTTMLHSEAKHPVCAYKWMSWSLTPKVQGDVAAWFGSLPVVPQGCKASPL
                     LGEKGCETNGFNYFDKIAFWKTPIAEGGKFVPYSRWTQDYIAIMGGR"
     gene            complement(8118..9524)
                     /gene="ydcR"
                     /locus_tag="ACHKBL_003107"
     CDS             complement(8118..9524)
                     /gene="ydcR"
                     /locus_tag="ACHKBL_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFO5819943.1"
                     /translation="MKKYQQLAEQLREQIASGIWQPGDRLPSLRDQVALSGMSFMTVS
                     HAYQLLESQGHIIARPQSGYYVATQAIKMPKAPVIPVTRDEAVDINTYIFDMLQASRD
                     PSVVPFASAFPDPRLFPLQQLNRSLAQVSKTATAMSVIENLPPGNAELRQAIARRYAL
                     QGITISPDEIVITAGALEALNLSLQAVTEPGDWVIVENPCFYGALQALERLRLKALSV
                     ATDVKEGIDLKALELALQEYPVKACWLMTNSQNPLGFTLTPQKKARLVALLNQYNVTL
                     IEDDVYSELYFGREKPLPAKAWDRHDGVLHCSSFSKCLVPGFRIGWVAAGKHARKIQR
                     LQLMSTLSTSSPMQLALVDYLSTRRYDAHLRRLRRQLAERKQRAWQALLRYLPAEVKI
                     HHNDSGYFLWLELPEPLDAGELSLAALTHHISIAPGKMFSTGENWSRFFRFNTAWQWG
                     EREEQAVKQLGKLIQERL"
     gene            complement(9603..10019)
                     /gene="hicB"
                     /locus_tag="ACHKBL_003108"
     CDS             complement(9603..10019)
                     /gene="hicB"
                     /locus_tag="ACHKBL_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HicB"
                     /protein_id="HFO5819944.1"
                     /translation="MRYPVTLTPAPEGGYVVSFVDIPEALTQGETVAEAMEAAKDALL
                     TAFDFYFEDNELIPLPSPLTNNDHFIEVPLSIASKVLLLNAFLQSEITQQELARRIGK
                     PKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV"
     gene            complement(10063..10239)
                     /gene="hicA"
                     /locus_tag="ACHKBL_003109"
     CDS             complement(10063..10239)
                     /gene="hicA"
                     /locus_tag="ACHKBL_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin HicA"
                     /protein_id="HFO5819945.1"
                     /translation="MKQSEFRRWLESQGVDVANGSNHLKLRFQGRRSVMPRHPSDEIK
                     EPLRKAILKQLGLN"
     gene            10461..10691
                     /gene="yncJ"
                     /locus_tag="ACHKBL_003110"
     CDS             10461..10691
                     /gene="yncJ"
                     /locus_tag="ACHKBL_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2554 family protein"
                     /protein_id="HFO5819946.1"
                     /translation="MFTKALSVVLLTCALFSGQLMAGHKGHEFMWVKNVDHQLRHEAD
                     SDELRAVAEESAEGLREHFYWQKSRKPEAGHR"
     gene            complement(10783..12744)
                     /gene="rlhA"
                     /locus_tag="ACHKBL_003111"
     CDS             complement(10783..12744)
                     /gene="rlhA"
                     /locus_tag="ACHKBL_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA 5-hydroxycytidine C2501 synthase"
                     /protein_id="HFO5819947.1"
                     /translation="MTVSSHRLELLSPARDAAIAREAILHGADAVYIGGPGFGARHNA
                     SNSLKDIAELVPFAHRYGAKIFVTLNTILHDDELEPAQRLITDLYQTGVDALIVQDMG
                     ILELDIPPIELHASTQCDIRTVEKAKFLSDVGFTQIVLARELNLEQIRAIHQATDATI
                     EFFIHGALCVAYSGQCYISHAQTGRSANRGDCSQACRLPYTLKDDQGRVVSYEKHLLS
                     MKDNDQTANLGALIDAGVRSFKIEGRYKDMSYVKNITAHYRQMLDAIIEERGDLTRAS
                     SGRTEHFFVPSTEKTFHRGSTDYFVNARKGDIGAFDSPKFIGLPVGEVLKVAKDHLDV
                     AVTEPLANGDGLNVMIKREVVGFRANTVEKTGENQYRVWPNEMPAELHKIRPHHPLNR
                     NLDHNWQQALTKTSSERRVAVDIELGGWQEQLILTLTSEEGVSITHTLDGQFDEANNA
                     EKAMNNLKDGLAKLGQTIYYARDVQINLPGALFVPNSLLNQFRREAADMLDAARLASY
                     QRGSRKPVADPAPVYPQTHLSFLANVYNQKAREFYHRYGVQLIDAAYEAHEEKGEVPV
                     MITKHCLRFAFNLCPKQAKGNIKSWKATPMQLVNGDEVLTLKFDCRPCEMHVIGKIKN
                     HILKMPLPGSVVASVSPDELLKTLPKRKG"
     gene            complement(12817..13353)
                     /gene="sutR"
                     /locus_tag="ACHKBL_003112"
     CDS             complement(12817..13353)
                     /gene="sutR"
                     /locus_tag="ACHKBL_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SutR"
                     /protein_id="HFO5819948.1"
                     /translation="MENLTRFLSTTLKQLRQQRGWSLSRLAEATGVSKAMLGQIERNE
                     SSPTVATLWKIATGLNVPFSTFISPPQSATPSVYDPQQQVMVITSLFPYDPQLCFEHF
                     SIQMAPGAISESTPHEKGVIEHVVVIDGQLDLCVDGEWQSLNCGEGARFAADVTHVYR
                     NGGEQIVHFHSLIHYPRS"
     gene            13406..14617
                     /gene="ydcO"
                     /locus_tag="ACHKBL_003113"
     CDS             13406..14617
                     /gene="ydcO"
                     /locus_tag="ACHKBL_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012601493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BenE family transporter YdcO"
                     /protein_id="HFO5819949.1"
                     /translation="MLRYLYAITHEVTMRLFSIPPPTLLAGFLAVLIGYASSAAIIWQ
                     AAIVAGATTAQISGWMTALGLAMGVSTLALTLWYRVPVLTAWSTPGAALLVTGLQGLT
                     LNEAIGVFIVTNALIVLCGITGLFARLMRIIPHSLAAAMLAGILLRFGLQAFASLDDQ
                     FTLCGSMLLVWLATKAVAPRYAVIAAMIIGVVIVIAQGDVVTTDVVFKPVLPTYISPD
                     FSFAHSLSVALPLFLVTMASQNAPGIAAMKAAGYSAPVSPLIVFTGLLALFFSPFGVY
                     SVGIAAITAAICQSPEAHPDKDQRWLAAAVAGIFYLIAGLFGSAITGMMAALPVSWIQ
                     MLAGLALLSTIGGSLYQALHNERERDAAVVAFLVTASGLTLVGIGSAFWGLIAGGVCY
                     VVLNLIANRNR"
     gene            complement(14652..>15199)
                     /locus_tag="ACHKBL_003114"
     CDS             complement(14652..>15199)
                     /locus_tag="ACHKBL_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
                     /protein_id="HFO5819950.1"
                     /translation="KETTSATGKPVLRWVDPSFDQSKYDSIVWNPVTYYPVPKPSTQV
                     GQKVLDKILSYTNTEMKEAIAQRKPLVTTAGPRSLIFRGAITGVDTSKEGLQFYEVVP
                     VALVVAGTQMATGHRTMDTRLYFEGELIDAATNKPVIKVVRQGEGKDLNNESTPMAFE
                     NIKQVIDDMATDATMFDVNKK"
BASE COUNT         3703 a         4011 c         3715 g         3770 t
ORIGIN      
        1 cccaccgtcg cccccacagg acctgtcgcc gcagcaacaa ccaatgtttc gccctctttg
       61 ggctgaccga tatccagcag ccccatataa gcggtaaagc ctggcatccc cagcacaccc
      121 agcgaccacg atggattttg cggatgatcg ccaagtttca ccagatcatc accactggat
      181 atgtcatagt cctgccatcc actgtagccc agcacccagt cgccaggctg gtaatcagga
      241 tgattcgact ccacaacacg gctcaccgta ccgccgacca ttacgccacc aatatcaacc
      301 ggcggtgaat aagatggctc atcgctcata cgtccacgca tatacgggtc cagtgacaaa
      361 taaactgtgc gcagtaacac ctgtccttca cccggtgtgg cgatatcatc ttcttcaaga
      421 cggaaattct ccggaaccgg cgcgccatgc ggacgcgagg ccagaaccca acgtcgatta
      481 cgctgctttt gttgccccat aatgctctcc tgtgcttttg gcatgaatcc atcaatagta
      541 gcaggctcgc aggaatggtt ttgtttgcag ccgtcacatc ccctctgcaa cacgcaataa
      601 tttcatcgag gaatccgttg cttcgggctg ttttcgatag tattgataac gtcatcatca
      661 ttatttaatc gaggccaaac atgtccatcc gttttgcccg caaagccgac tgtgctgcca
      721 ttgcggaaat ttataaccac gccgtgttgt atacggcggc tatctggaat gaccaaacgg
      781 tggatgcaga taaccgcatt gcctggtttg aagcgcggac catagcaggt tatccagtgc
      841 tggtgagcga agaagatggc gtagtgacgg gatatgcctc gtttggcgac tggcgtagtt
      901 tcgatggttt tcgccatacc gtggaacatt cggtttatgt ccatcccgat catcagggca
      961 aaggtctggg gcgtaaattg ttaagccgat tgattgatga agcgcgggat tgcgggaagc
     1021 atgtcatggt cgccgggatc gaatcgcaaa atcaggcgtc tttgcatctg caccagtcgc
     1081 tgggatttgt cgtcaccgcg caaatgccgc aggtgggcac taaatttggg cgctggctgg
     1141 acctgacatt tatgcagttg caactcgacg aacgcagtga accggacgca atctgatgaa
     1201 tcagtcgctc acccttgctt ttttgattgc cgccgggatt ggtctggtag tccagaacac
     1261 gctgatggtg cgcatcaccc agacctcctc taccattctc atcgccatgt tgctgaactc
     1321 tttggtagga attgtgctct ttgtttctat tttatggttt aaacaaggta tggcggggtt
     1381 tggcgaactg gtgtccaacg tgcgctggtg gacacttata cctggcttgc tgggatcgtt
     1441 ttttgtcttc gccagtatca gcggatatca gaatgtggga gcggcgacaa ctattgcggt
     1501 actggtcgcc agccagctga ttggcggtct ggtgctggat atcttccgca gccatggcgt
     1561 accactacgg gcgttgatcg gcccaatttg tggcgcaatt ttacttgtgg ttggtgcctg
     1621 gctggtggcc aggcgcacat tttaatcaga ggatggtgcc acctttggtt aactgttcgt
     1681 ggcgcgcttc catatcttct ttatgtttgc gaccgtgatg ggcgatcgct gtgcgaagac
     1741 gctgctgctg ggtatagcgt tcttcccgac ttaactccgc atcatcgctc agggcgatta
     1801 ataattcgtt catatgggca atgacgctct cttcaatcgc ggcatccacg cgcgcgatga
     1861 cttcatccag atgcgacata ctttctcctt agccacttgt gaaatttatc gattcatcca
     1921 aatgatataa gtctgcgtgg tgccgtcaag ccagtgagta agatttaaaa cagtgaaaac
     1981 aacagcgcca ccgggaaact catcggccat gttgccccca ccagaaaggc gcttaagaaa
     2041 cggatgcgtt tcttatcgtg agaaagaaac caggtgataa gaaatgcgat tgctgccata
     2101 accgcataaa aaacaagcat gtgttgatag agagacatgt acgggcgtcc agccagatga
     2161 ataaaacgcg cgcaaatatg cgtcattttt tgacgtacat caagtttttt aaccggtttg
     2221 agaaaaaaac atcaaacagc aacttgccct tatccatctt cagaatgagt attattgcgg
     2281 attttcttaa cccgtactat acacataggg gcaatgataa aaggtggcaa aaatgaatgt
     2341 ttccagtaga actgtagtac tgataaatgt ctttgctgct gttggtttgt ttactcttat
     2401 ctctatgaga tttggctggt ttatttgatg tagtgatttt gcacgcagca cgtactgtca
     2461 gagaaatcag tgctctgaca gtacgcaaga tcatctcata ctcttacgat ctgcaatatt
     2521 atccttaatg tttaaccatg acgtggcgga cgacggtgta atcctccagc ccataaagtg
     2581 acatatcctt gccgtaacca gaaagtttct gcccaccgtg cggcatttca cttaccagca
     2641 tgaaatgggt attgacccag gtacaaccat attgcagccg tgcgctgacg cgatgcgccc
     2701 tgcccacatc tttcgtccat accgaagatg caagtccgta ctggctgtca ttcgcccagt
     2761 ttaccacctg ttcttcgttg tcgaagagtg taacactcac tactggacca aatacctctt
     2821 tttgcacgat ggcatcgtcc tgtaatgcgc cagccagcag cgtcggcgca tagtaatagc
     2881 cattaccctt gcgcttttca ccgccagtga tcactttgat gtgccctgtc gctttcgcct
     2941 cttctactgc cttgctgacg cgttcgagat gcgccagcga gcttaaaggt ccaagctccg
     3001 tagactcgtc atccggcgca ccagatttta acgttgccac cgcagcaccc agtttttcca
     3061 ccagcgtatc gtaaatgcct ttttgcgcgt agatccgaca agccgcagta caatcctgtc
     3121 cggcattgta atagccaaat gtacgtacac cttcgaccac tgcctcaata tccgcatcat
     3181 caaaaacaat cactggcgct ttgccaccaa gttccatatg agtacgcttt atggacggcg
     3241 cggtatggct gatgatgtgt tccccagtgg cgatagagcc cgtcagcgac accatccgca
     3301 ctttgggatg accggtcagc ggatcgccca ccgttttgcc tctgccaaac agtacattaa
     3361 tcacgccagc cgggaagata tctttcgcca actctgccaa cttcaacgct gtcagcgggg
     3421 taatttctga tggtttaagc actacgcagt tccctgccgc cagcgccgga gccagtttcc
     3481 acgcagccat catcagagga taattccacg gtgcgataga agccacgccc cccaacggat
     3541 cgcgacggat catcgaagta tgaccttcaa gatattcacc tgccgccaga ccattcagac
     3601 agcgcgccgc accagcgaaa aagcgaaaaa catcgacaat cgccgggatt tcatcattga
     3661 acgcactatg cagcggtttg ccacaattac gggactccag ttcggcaaaa acctgaccat
     3721 tttcttcgat aacatcagcc agtttcagca gacattccgc acgcactttc ggcgtggttt
     3781 gcccccattc ggcaaatgct gcatctgccg cgcgaacagc agcatcgacc tgctctgcgg
     3841 atgcctcggc aatctccagt aaaacgtccc ccgttgccgg attatagaca ggctgttttt
     3901 ccccttcgcc gctaaccagt tctccgttaa tcagtaactt atgttgcata gcatattcct
     3961 gtatcagtgg ttattgacca ttgtcgcctt cgcgggttag ccaccaggcc cccaggatcg
     4021 gcaaggttgt taccaacata accagcagtg ccaccacgtt agttaccggt acatcacgcg
     4081 gtcgcccaag ctgattgagc aaccacaacg gtaacgttcg ttcatgacct gccgtaaagg
     4141 tcgtaacgat gatttcatcg aacgacaagg caaacgccag cattcctcct gccagtaacg
     4201 ccgaactgag attcggcaac actacgtagc ggaaggtttg ccagccatta gccccaagat
     4261 ccattgacgc ctcaaccaga ctccacgagg tgcggcgaaa acgggcgatg acattgttaa
     4321 agaccaccac tacacaaaaa gtcgcatgac cgaccacgat ggtgaaaaat cccggctcca
     4381 gattgatggt tttaaaggcg gttaataacg ccagaccagt gacaatgccc ggcagcgcaa
     4441 tgggcagcag cagtaacagc gaaatggcgt ttttgccgaa aaagtctcgt cgccacagcg
     4501 cggcagcggc cagcgtccct aacaccagcg cgattaatgt cgccagcgcc gccactttaa
     4561 gtgacagtgt cacggcatca agaatatcac tacgctgtgc tgccacgcta aaccagcgca
     4621 gcgtcaggcc ttgcggtgga aaactaaacg ccgcatcttc agtgttaaac gcataaacgg
     4681 cgataatcag aatgggaaaa tgtaagaaaa ccacgccgcc ccaggccgcc agtttgagga
     4741 aaaacggtgc gcgttcagag tgcatcgaac gctcccagac gtttcacgaa cgctaggtaa
     4801 agtgcgatga gtacaatcgg cactagggtg aatgccgccg ccatcggcat attgccaatc
     4861 gccccctgct gggaataaac catattgccg ataaaatatc ctggagggcc aaccagctgc
     4921 gggacaataa aatcgcccag cgtgagtgag aaggtaaaga tagagccagc ggcaataccc
     4981 gggattgcca gcggcagcac cacatagcga aaggtttgtc gtggacgtgc gccgagatca
     5041 gctgacgcct gcaacaatga cggcggcaaa cgctcaagcg ccgcctgcac cgggaggatc
     5101 atgaacggca accagatata gagaaacacc agaaagcgcc ccagcccgga agttgacagc
     5161 gtattacctc ccaccgccgg tagcgttagg aacgcagtca gcagtggttc cagccccaga
     5221 tgttgtaaaa accactgcgc cacgccatct tttgccagca ataacgtcca ggcataggct
     5281 ttcacaatgt agctcgccca catcggcaac attaccgcga tataaaaaaa cgctttcatt
     5341 ttcccgctgg tatagcgcgc catataccac gccattggaa aagccagaat ggcgctggcg
     5401 atagtgactg ccacagccat aatcagcgtg cggagaatga tgtcgtaatt cgccggatta
     5461 aacagcgcac ggatattcgc cagcgtcagt tccggcgtta ccgacatggt gaaatcgtca
     5521 aaggtataaa atccctgcca taacagtgtc agcagcgagc cgaaatagac aatgccaaac
     5581 cacattagcg ggccaagcag cagtaaaaac agccccagcc ccggattacc ccagaagaaa
     5641 ccggacacct tacccagacc tggacgtgaa ggtgattgca atacgttcat cgccattcac
     5701 ctctcctcaa ccagcggtac catcacatca cgcgaccagg aaaccatcac ctgttgtccg
     5761 ggcgtgagcg tggcgggcag ttcttcgcct gtcatattgg cctgactcac cagcagtttt
     5821 tcaccgccgt tcagtctcag ttcaaaacgg gtcgccgcgc cctgatactg caccgcctgg
     5881 atcgtgccat tagcctgcac ttcgccgggg atgttgaagc ggatatgttc cggtcgcagg
     5941 gcgaagcttc ccgtcatgcc acaaagtttg tctgccatca gtgcatcaaa aacattcgat
     6001 gtcccgacga acccggcaac aaacggcgtg cgcgggcgca tatagagatc acgaggggaa
     6061 tcgacctgct caatgcgtcc attattgaaa accgccacac gatcggacat cgataacgct
     6121 tcgccctgat cgtgggtaac gaagataaaa gtgataccga gagactgttg cagttttttc
     6181 agttccagct gcatctgctc acgcaatttg agatccagtg cgccgagcgg ttcatccaac
     6241 agcaatacgc gcggttcatt caccaatgct ctggcgatag caacccgctg gcgctgacca
     6301 ccagaaagct gtgacggctt acgttgctga acaaaaccaa gcgccacttt ctccagcgct
     6361 tcttgcgcca tagcatatcg ctgtttttta tccacgcctt tgaccatcag cccataggcc
     6421 acgttgtcaa gaatcgacat atgcggaaaa agtgcgtagt cctggaagac ggtattaaca
     6481 tcccgctccc acggtggcag attgctggcg gatttaccaa agatagagat agccccgccg
     6541 gaaagctgtt cgaagccagc aatcaggcgc aggcaggtgg ttttgccgga gccggacggc
     6601 cccagcatag agaagaactc accatctttt atcgcaatac tgacgccatc tactgcgcgc
     6661 acgtcaccgt acaaccgtga gacgttgtca aactccactg cgtacgtcat aaaacacccc
     6721 agcgaagtta gcgaccgccc ataatggcaa tgtaatcctg cgtccagcga ctgtagggaa
     6781 caaacttgcc cccttctgct ataggcgttt tccagaaggc gattttatcg aaatagttaa
     6841 aaccgttggt ttcacaacct ttttcaccta ataacggact ggctttacac ccttgcggca
     6901 ctactggtaa cgagccaaac caggccgcca catcaccctg cacttttggc gttaatgacc
     6961 agctcatcca tttgtaggcg caaaccggat gtttcgcttc gctatgtagc atggtggtgt
     7021 cagcccaacc ggtaacgccc tccttcggga aaacggtagc gacaggctgg ccttcggctt
     7081 tcagggcgtt ggcctgatag ggccatgcac tggaagcaac cacaccctcg tttttgaaat
     7141 cgctcatttg cacggtagtg tcatgccagt agcgatggat caaactatgt tgatcgcgca
     7201 tcactttcag taccgcctga tactgttctt cggtgagttg atacggatcg cggatgccca
     7261 actgcggctg agtggcttta acgaacaacg cagcgtccgc aatgtagata gggccatcat
     7321 aagcctgaac gcggccttta ttgctcttgc cgtccggcag attctgctca acaaaaacca
     7381 cttgccagct atccggcggc gtcgggaagg ttttagtgtt gtacatcagc aggttcggcc
     7441 cccattggta aggtgtgccg taaactttgc cgccaacgtt aaaccagtcg cctttaacca
     7501 cgcgcggatc gagcgctttc cagttgggaa tcaaggcggt attaattggc tgcacgcgtt
     7561 tacccataat caaacgcagc gaggcatcgc cggatgccgt aaccagatcg taaccccctt
     7621 tggccatcag actgaccatt tcatcggaag tcgcggcggt tttaacattc accgcgcagc
     7681 ctgtctcttt ttcgaattgc gttacccagt cgtattgttt atcagtttgt ccgcgttcga
     7741 tgtatcccgg ccaggcgata atatccagtc gcccttccgg tttatctaaa ttggtaggtg
     7801 gttcggcggc gtgagcggtc attattgtca tggtgagcgc acacaggctg ctgcgggcaa
     7861 atgtcttgct cataaagtct tactcctgtc gtaattaatt gttcagccgc cgtgccgtaa
     7921 aaatatctcc ttgtttaacc atagacggcg gctagcaggc acaccttcgc ccctgagaaa
     7981 atttaatcag gttgtgagct accgcgcatt atgccgtatg taaggttatc ttctggagta
     8041 tagacaagga gttaggaaat atgactgcta ttcgaaattc atattaccca acctgagagg
     8101 aatttaatta aacgctatta cagccgttct tgaataagtt tgcctaattg tttaaccgcc
     8161 tgttcttcac gttctcccca ctgccacgcg gtattaaaac ggaaaaaacg tgaccagttt
     8221 tcaccggtag aaaacatttt acccggcgca atactgatat gatgcgtcag tgctgccagg
     8281 cttaattcgc ccgcatctaa cggctcgggg agctccagcc agagaaaata accgctgtca
     8341 ttatgatgaa ttttcacttc cgcaggcaga taacgcagca gtgcttgcca ggcacgttgt
     8401 ttacgttccg caagctggcg acgcaggcga cgaagatggg cgtcgtatcg gcgcgtggaa
     8461 aggtaatcca ccagcgcaag ctgcatcggt gagctggtgg aaagcgtact catcaactgc
     8521 aagcgttgaa ttttacgtgc atgttttccg gcggcgaccc aaccaatacg aaaaccaggc
     8581 accagacatt tcgaaaacga agagcaatgc aaaacaccat cgtggcgatc ccacgctttc
     8641 gcaggcagcg gtttttcccg tccaaaataa agttcgctgt aaacgtcatc ttcaatcagc
     8701 gttacgttgt actgattgag caacgctacc agccgcgctt ttttctgcgg cgttaaggta
     8761 aatccgagtg gattctggct attagtcatc agccagcacg ctttcaccgg atactcctgc
     8821 aacgccagtt ccagcgcctt gagatcaatc ccttctttaa cgtccgtcgc caccgataac
     8881 gccttcagcc gtaaccgctc cagcgcctgc aacgcaccgt agaaacaagg attctctact
     8941 atcacccaat cgcccggttc agtgaccgct tgtaaactga ggtttaatgc ctctaacgcc
     9001 ccggcagtaa tgacaatttc atcgggtgaa atggtgatgc cctgtaaggc atagcgacga
     9061 gctatagcct gacgcagttc agcgtttcct ggcggtaagt tttcaatcac gctcatcgcc
     9121 gtggcggttt tgcttacctg tgccagcgag cggttgagtt gttggagggg gaaaagtcgc
     9181 gggtcgggaa aggccgaggc aaacggaact accgacggat cgcgactggc ctgcaacata
     9241 tcaaaaatat aggtgttgat atcgactgct tcatctcgag tgactggaat gactggcgct
     9301 ttcggcattt ttattgcctg tgtcgcaacg taataacccg actgcggtcg cgcgataata
     9361 tgtccctgac tttcgagcaa ctgataggca tggctgacag tcataaagct catgccagaa
     9421 agcgccacct ggtcacgcaa cgaaggcaaa cgatcgccag gttgccagat acccgacgca
     9481 atctgctcgc gtaattgttc tgcaagctgc tggtattttt tcattatcga atcgtaatta
     9541 tgtgcagatg attcggcagt ctatatcagt tgttaaaaat gacaactttt cattaactgt
     9601 tatcaaacca tcaccagtga taactctttg ccaagcgcct tagccgcaag ctggacggcg
     9661 tcgatttttg tagcatggtg caagttaaat aagcgagtaa tctcctgttt aggtttgcca
     9721 attcgtctgg ctaactcttg ctgagtgatt tctgactgta aaaaagcatt taacagcaat
     9781 actttagagg cgatacttaa aggcacttca ataaagtgat cgttgttagt taatggcgaa
     9841 ggcaacggga ttagctcatt atcttcaaaa taaaaatcaa atgcggtgag taaggcatct
     9901 tttgctgctt ccatcgcttc agcaacagtt tctccctgcg tcaacgcttc agggatatct
     9961 acaaaagaaa ccacataacc gccttccggc gcaggagtaa gagtgacggg ataacgcata
    10021 attttgactg tctctcgcat gtcattaacc ttcttatggc gattaattta aaccgagttg
    10081 cttcaggatt gctttacgca atggttcttt tatctcatca ctggggtgac gcggcatgac
    10141 actgcgtcgc ccttgaaacc tgagtttcaa atggttgctg ccattcgcca catcgacgcc
    10201 ctgagattcg agccaacgtc tgaactcgct ttgtttcaca tcgcctccct gccgctgagt
    10261 atatcaacaa agtaaacatt agtgtttacc tccgtgtgta atttgctcga aaatagcact
    10321 aaatcgccgc aacggcagaa tgcaatgcaa aatcaggaag gaagattcca gaatttcttt
    10381 gcgctaattt gcaaaactga ataataacct caagaaacga tcggcatcat agcgacaaaa
    10441 ggtaagaaaa aggtggcagg atgtttacga aggcgttatc ggttgtctta ttaacgtgtg
    10501 cactgttctc aggacaactc atggcagggc acaaaggaca tgaatttatg tgggtaaaga
    10561 atgtggatca tcagctgcgt catgaagcgg acagcgatga attgcgtgct gtggcggaag
    10621 agtcggcgga aggtttgcgc gagcattttt actggcaaaa atcgcgcaaa ccagaagcgg
    10681 gacatcgttg agtcatttgt tgccggatgc ggcatgacgc cttacccggc aggcaatatc
    10741 atgcagattt gatgagttgt gagtaaccag aaaactggcg ttttaccctt ttcgcttcgg
    10801 taaagttttc agcagctcat ccggacttac ggatgccact acgcttcccg gtaacggcat
    10861 tttcagtatg tgatttttga ttttgccaat gacgtgcatc tcgcatgggc ggcaatcaaa
    10921 ctttagcgtt aatacttcat cgccgttaac cagttgcatt ggcgtcgcct tccagctttt
    10981 gatattgcct ttcgcctgtt tcgggcacag attgaaggca aagcgcagac aatgcttagt
    11041 gatcatcacc gggacttcgc ccttctcttc atgcgcttca tacgccgcat caatcagttg
    11101 cacaccatag cgatgataaa attcacgcgc tttctggttg taaacgttcg cgaggaaact
    11161 caaatgcgtt tgcggataaa ccggcgcagg atcagcaacc ggtttacggc tgccgcgctg
    11221 gtaactggca agacgcgcag catctagcat gtcggcggct tcacggcgga actggtttaa
    11281 cagactgttt ggtacaaaca gcgcacccgg caaattaatt tgcacatcgc gggcgtaata
    11341 gatggtttgc cccagttttg ccagaccatc ctttagattg ttcattgctt tttcggcgtt
    11401 attggcttcg tcgaactgcc cgtccagcgt atgcgtgatg ctgacaccct cttcactggt
    11461 gagggtcaga atcagttgtt cctgccagcc gcccagttca atgtctaccg ccacccgacg
    11521 ttcgctggag gtttttgtca gtgcctgctg ccagttatga tcaagattac ggtttagtgg
    11581 gtgatgcggg cgaattttgt gcaactctgc tggcatttca ttgggccaga cgcggtactg
    11641 attttctccg gttttctcga ccgtattggc acgaaagccg acgacttcac gtttaatcat
    11701 cacgttcagg ccatcgccat ttgccagtgg ctcggtaacg gcaacatcga gatgatcttt
    11761 cgccactttc aatacttcac ccaccggcag gccgataaat ttcggcgaat cgaacgcgcc
    11821 aatatcgcct ttacgggcat tcacaaaata atctgtgcta ccacggtgga aagtcttttc
    11881 cgtcgatgga acaaagaaat gttcagtacg acctgatgaa gcacgcgtca gatcgccacg
    11941 ttcttcaata atggcatcca gcatctggcg ataatgggcg gtgatattct tcacgtagct
    12001 catatctttg taacgccctt caatcttgaa ggagcgtacg ccagcatcaa tcagcgcgcc
    12061 gaggttggca gtctgatcgt tatctttcat cgacagcaga tgtttttcat aggaaaccac
    12121 ccgcccctga tcgtctttca atgtgtatgg caaacggcac gcctgcgagc aatcgccacg
    12181 gttggcgcta cgccctgttt gcgcatgaga aatgtagcac tgacccgaat aggccacgca
    12241 cagtgcgcca tgaataaaga attcaatggt cgcgtccgtg gcctggtgaa tcgcgcggat
    12301 ctgctcaaga ttcagctctc gcgccagcac aatctgcgtg aagccaacat cagagaggaa
    12361 cttcgctttt tctactgtac gaatgtcgca ctgcgtactg gcgtgcagtt caatcggcgg
    12421 aatatcaagt tccagaatcc ccatatcctg aacaatcagc gcgtcgacgc cggtctggta
    12481 gaggtcggta atcagccgtt gcgcaggttc cagctcatca tcatgcaaaa tggtgttaag
    12541 cgtgacgaaa atttttgcac cataacgatg ggcaaatggc accagttcgg caatatcttt
    12601 caggctatta ctggcattat gacgggcacc aaaaccaggg ccgccgatat aaacagcatc
    12661 ggcaccgtgc aaaatagctt cgcgggcaat ggcggcatcg cgtgccgggc ttaacagttc
    12721 aagtcgatga gaagatacgg tcacattctt atccagtagc tgaaaaatgg cggctatttt
    12781 agccacaagc gcgacgttgc gaaatagttt tcccgcttaa ctgcgtgggt aatggatgag
    12841 ggaatgaaaa tgtacgattt gctccccacc attacgatag acatgcgtaa cgtctgcagc
    12901 aaatcgagcg ccttctccac aattaaggct ctgccattca ccatcaacgc acagatcaag
    12961 ttgcccatca atgacgacaa catgttcgat tactcccttc tcatggggcg tcgattcact
    13021 tattgcacca ggtgccatct gaatcgagaa gtgttcaaag cagagttgtg gatcataagg
    13081 aaacagcgaa gtgatcacca tcacctgctg ttgcggatca taaactgaag gcgtagcgga
    13141 ctgcggcgga gaaataaatg tcgaaaatgg cacattcaac ccggtggcaa ttttccataa
    13201 tgtcgcgacc gtcgggctgg actcattacg ctcaatctgc cctaacatcg ctttagaaac
    13261 gcccgtcgct tctgccagtc gcgaaagact ccagccgcgc tgctgacgta gttgtttaag
    13321 tgtggtggat aaaaagcgag tgagattttc catgcgactc ctccggcaaa acggaagttt
    13381 atcacttgtg cgttataacg gacaaatgct acggtacctg tatgctataa cgcacgaggt
    13441 gaccatgcgt ctgttttcta ttcctccacc cacgctactg gcggggtttc tggcggtatt
    13501 aattggctac gccagttcag cggcaataat ctggcaagca gcgattgtcg ccggagccac
    13561 cactgcacaa atctctggct ggatgacggc gctggggctg gcaatgggcg tcagtacgct
    13621 ggctctgaca ttatggtatc gcgtacctgt tctcaccgca tggtcaacgc ctggcgcggc
    13681 tttgttggtc accggattac agggactaac acttaacgaa gccatcggcg tttttattgt
    13741 caccaacgcg ttaatagtcc tctgcggcat aacgggactc tttgctcgtc tgatgcgcat
    13801 tattccgcac tcgcttgcgg cggcaatgct tgccgggatt ttattacgct ttggtttaca
    13861 ggcgtttgcc agtctggatg atcaatttac gttgtgtggc agtatgttgc tggtatggct
    13921 ggcaaccaag gccgttgcac cgcgctatgc ggtaattgcc gcgatgatta ttggggtcgt
    13981 gatcgttatc gcgcaaggtg acgttgtcac aactgatgtt gtctttaaac ccgttctccc
    14041 cacttatatt agccctgatt tttcgtttgc tcacagcctg agcgttgcac tccccctttt
    14101 tctggtgacg atggcatcgc aaaacgcacc gggtatcgca gcaatgaaag cagctggata
    14161 ttcggctcct gtttcgccat taattgtgtt tactggattg ctggcactgt ttttttcccc
    14221 tttcggcgtt tattccgtcg gtattgcggc aatcaccgcg gctatttgcc aaagcccgga
    14281 agcgcatccg gataaagatc aacgttggct ggccgctgcc gttgccggca ttttttattt
    14341 gatcgcaggt ctgtttggta gtgccattac cgggatgatg gctgccctgc ccgtaagttg
    14401 gatccagatg ctggcaggtc ttgcgctgtt gagtaccatt ggcggcagtt tgtatcaggc
    14461 gctgcataat gagcgtgagc gcgacgcggc ggtggtggca tttctggtaa cggcaagtgg
    14521 attgacgctg gtcgggattg gttctgcgtt ctggggatta attgccggag gcgtttgtta
    14581 cgtggtgttg aatttaatcg ctaacagaaa ccgataataa aaagcgccat ctaacgatgg
    14641 cgcaactggt attacttttt gttaacgtca aacatggtgg catcggtcgc catgtcatca
    14701 ataacttgtt taatattttc gaaagccatt ggcgtacttt cgttattcag gtctttacct
    14761 tcgccctgac gaacgacttt gataaccggt ttattagtcg ctgcatcaat cagctcacct
    14821 tcaaaataca ggcgagtatc catggtacgg tggcccgtcg ccatttgcgt ccccgcaacc
    14881 actaatgcaa caggaaccac ttcatagaat tgcagccctt ctttgctggt atctacaccg
    14941 gtaatggccc cacggaaaat cagactacgc ggcccagcgg tggtaaccag tggtttacgc
    15001 tgcgcaatcg cttctttcat ttcggtgttg gtataactca aaattttatc cagaactttc
    15061 tgccctacct gggtagatgg tttcggtacc ggataataag tgactgggtt ccagacgatg
    15121 ctgtcatatt tgctttgatc aaaactcggg tctacccaac gtaaaacagg tttacctgtt
    15181 gctgaggttg tttctttta
//
LOCUS       DAZFLD010000120        15078 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.121, whole genome shotgun sequence.
ACCESSION   DAZFLD010000120 DAZFLD010000000
VERSION     DAZFLD010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15078)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15078)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15078
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.121"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            98..1021
                     /gene="yicL"
                     /locus_tag="ACHKBL_003115"
     CDS             98..1021
                     /gene="yicL"
                     /locus_tag="ACHKBL_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylate/amino acid/amine transporter"
                     /protein_id="HFO5819951.1"
                     /translation="MGSTRKGMLNVLIAAVLWGSSGVCAQYIMEQSQMSSQFLTMTRL
                     IFAGLILLTLSFVHGDKIFSIINNHKDAISLLIFSVVGALTVQLTFLLTIEKSNAATA
                     TVLQFLSPTIIVAWFSLVRKSRPGILVFCAILTSLIGTFLLVTHGNPTSLSISPAALF
                     WGIASAFAAAFYTTYPSTLIARYGTLPVVGWSMLIGGLILLPFYARQGTNFVVNGSLI
                     LAFFYLVVIGTSLTFSLYLKGAQLIGGPKASILSCAEPLSSALLSLLLLGITFTLPDW
                     LGTLLILSSVILISMDSRRRARKINRPARHE"
     gene            complement(1025..1843)
                     /gene="nlpA"
                     /locus_tag="ACHKBL_003116"
     CDS             complement(1025..1843)
                     /gene="nlpA"
                     /locus_tag="ACHKBL_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpA"
                     /protein_id="HFO5819952.1"
                     /translation="MKLTTHHLRAGAALLLAGILLAGCDQSSSDEKHIKVGVINGAEQ
                     DVAEVAKKVAKEKYGLDVELVGFSGSLLPNDATNHGELDANVFQHRPFLEQDNQAHGY
                     KLVAVGNTFVFPMAGYSKKIKTVAQIKEGATVAIPNDPTNLGRALLLLQKEKLITLKE
                     GKGLLPTALDITDNPRHLQIMELEGAQLPRVLDDPKVDVAIISTTYIQQTGLSPVNDS
                     VFIEDKNSPYVNILVAREDNKNAENVKEFLQSYQSPEVAKAAETIFNGGAVPGW"
     gene            2065..2358
                     /gene="yicS"
                     /locus_tag="ACHKBL_003117"
     CDS             2065..2358
                     /gene="yicS"
                     /locus_tag="ACHKBL_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YicS"
                     /protein_id="HFO5819953.1"
                     /translation="MKPTMLLMITVFLIFPAISQAESPFSSLQSAKEKTTVLQDLRKI
                     CTPQASLSDEAWEKLMLSDENNKQHIREAIVAMERNNQSNYWEALGKVECPDM"
     gene            complement(2399..3589)
                     /gene="nepI"
                     /locus_tag="ACHKBL_003118"
     CDS             complement(2399..3589)
                     /gene="nepI"
                     /locus_tag="ACHKBL_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015211 - purine nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015860 - purine nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine ribonucleoside efflux pump NepI"
                     /protein_id="HFO5819954.1"
                     /translation="MSEFIAENRGADAITRPNWSAVFSVAFCVACLIIVEFLPVSLLT
                     PMAQDLGISEGVAGQSVTVTAFVAMFASLFITQTIQATDRRYVVILFAVLLTLSCLLV
                     SFANSFSLLLIGRACLGLALGGFWAMSASLTMRLVPPRTVPKALSVIFGAVSIALVIA
                     APLGSFLGELIGWRNVFNAAAAMGVLCIFWIIKSLPSLPGEPSHQKQNTFRLLQRPGV
                     MAGMIAIFMSFAGQFAFFTYIRPVYMNLAGFGVDGLTLVLLSFGIASFVGTSLSSFIL
                     KRSVKLALAGAPFVLALSALVLTLWGSDKIVATGVAIIWGLTFALIPVGWSTWITRSL
                     ADQAEKAGSIQVAVIQLANTCGAAIGGYALDNIGLTSPLMLSGTLMLLTALLVTAKVK
                     MKKS"
     gene            complement(3800..4252)
                     /gene="yicN"
                     /locus_tag="ACHKBL_003119"
     CDS             complement(3800..4252)
                     /gene="yicN"
                     /locus_tag="ACHKBL_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1198 domain-containing protein"
                     /protein_id="HFO5819955.1"
                     /translation="MIWIMLATLAVVFVVGFRVLTSGARKAIRRLSDRLNIDVVPVES
                     MVDQMGKSAGDEFLRYLHRPDESHLQNAAQVLLIWQIVIVDGSEQNLLQWHRILQKAR
                     LAAPITDAQVRLALGFLRETEPEMQDINAFQMRYNAFFQPAEGVHWLH"
     gene            complement(4305..5639)
                     /gene="adeQ"
                     /locus_tag="ACHKBL_003120"
                     /gene_synonym="yicO"
     CDS             complement(4305..5639)
                     /gene="adeQ"
                     /locus_tag="ACHKBL_003120"
                     /gene_synonym="yicO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeQ"
                     /protein_id="HFO5819956.1"
                     /translation="MNNDNTDYVSNESGTLSRLFKLPQHGTTVRTELIAGMTTFLTMV
                     YIVFVNPQILGAAQMDPKVVFVTTCLIAGIGSIAMGIFANLPVALAPAMGLNAFFAFV
                     VVGAMGISWQTGMGAIFWGAVGLFLLTLFRIRYWMISNIPLSLRIGITSGIGLFIALM
                     GLKNTGVIVANKDTLVMIGDLSSHGVLLGILGFFIITVLSSRHFHAAVLVSIVVTSCC
                     GLFFGDVHFSGVYSIPPDISSVIGEVDLSGALSLELAGIIFSFMLINLFDSSGTLIGV
                     TDKAGLIDSNGKFPNMNKALYVDSVSSVAGAFIGTSSVTAYIESTSGVAVGGRTGLTA
                     VVVGVMFLLVMFFSPLVAMVPPYATAGALIFVGVLMTSSLARVNWDDFTESVPAFITT
                     VMMPFTFSITEGIALGFMSYCIMKVCTGRWRDLNLCVVVVAALFALKIILVD"
     gene            5814..7580
                     /gene="adeD"
                     /locus_tag="ACHKBL_003121"
     CDS             5814..7580
                     /gene="adeD"
                     /locus_tag="ACHKBL_003121"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HFO5819957.1"
                     /translation="MNNSINHKFHHISRAEYQELLAVSRGDAVADYIIDNVSILDLIN
                     GGEISGPIVIKGRYIAGVGAEYADAPALQRIDARGATAVPGFIDAHLHIESSMMTPVT
                     FETATLPRGLTTVICDPHEIVNVMGEAGFAWFARCAEQARQNQYLQVSSCVPALEGCD
                     VNGASFTLEQMLAWRDHPQVTGLAEMMDYPGVISGQNALLDKLDAFRHLTLDGHCPGL
                     GGKELNTYIAAGIENCHESYQLEEGRRKLQLGMSLMIREGSAARNLNALAPLINEFNS
                     PQCMLCTDDRNPWEIAHEGHIDALIRRLIEQHNVPLHVAYRVASWSTARHFGLNHLGL
                     LAPGKQADIVLLSDARKVTVQQVLVKGEPIDAQTLQAEESARLAQSAPPYGNTISRQP
                     VSASDFALQFTPGKRYRVIEVIHNELITHSRSSVYSENGFDRDDVCFIAVLERYGQRL
                     APACGLLGGFGLNEGALAATVSHDSHNIVVIGRSAEEMALAVNQVIQDGGGLCVIRNG
                     QVQSHLPLPIAGLMSTDTAQSLAEQIDALKAAARECGPLPDEPFIQMAFLSLPVIPAL
                     KLTSQGLFDGEKFAFTTLEVTE"
     gene            complement(7627..9018)
                     /gene="uhpT"
                     /locus_tag="ACHKBL_003122"
     CDS             complement(7627..9018)
                     /gene="uhpT"
                     /locus_tag="ACHKBL_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HFO5819958.1"
                     /translation="MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYL
                     IRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGVGKTLVSYYADGKNTKQFLPFML
                     ILSAICMLGFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
                     LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPE
                     SYGLGKAEELFGEEISEEDKETESTDMTKWQIFVEYVLKNKVIWLLCFANIFLYVVRI
                     GIDQWSTVYAFQELKLSKAVAIQSFTLFEAGALVGTLLWGWLSDLANGRRGLVACIAL
                     ALIIATLGVYQHASNEYIYLASLFALGFLVFGPQLLIGVAAVGFVPKKAIGAADGIKG
                     TFAYLIGDSFAKLGLGMIADGTPVFGLTGWAGTFAALDIAAIGCICLMAIVAVMEERK
                     IRREKKIQQLTVA"
     gene            complement(9274..10593)
                     /gene="uhpC"
                     /locus_tag="ACHKBL_003123"
     CDS             complement(9274..10593)
                     /gene="uhpC"
                     /locus_tag="ACHKBL_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HFO5819959.1"
                     /translation="MLPFLKAPADAPLLTDKHEIDARYRYWRRHILLTIWLGYALFYF
                     TRKSFNAAVPEILANGVLSRSDIGLLATLFYITYGVSKFVSGIVSDRSNARYFMGIGL
                     IATGIINILFGFSTSLWAFAVLWVLNAFFQGWGSPVCARLLTAWYSRTERGGWWALWN
                     TAHNVGGALIPIVMAASALHYGWRAGMMIAGCMAIVVGIFLCWRLRDRPQALGLPAVG
                     EWRHDALEIAQQQEGAGLTRKEILTKYVLLNPYIWLLSFCYVLVYVVRAAINDWGNLY
                     MSETLGVDLVTANTAVTMFELGGFIGALVAGWGSDKLFNGNRGPMNLIFAAGILLSVG
                     SLWLMPFASYVMQATCFFTIGFFVFGPQMLIGMAAAECSHKEAAGAATGFVGLFAYLG
                     ASLAGWPLAKVLDTWHWSGFFVVIAIAAGVSALLLLPFLNAQTPREA"
     gene            complement(10603..12105)
                     /gene="uhpB"
                     /locus_tag="ACHKBL_003124"
     CDS             complement(10603..12105)
                     /gene="uhpB"
                     /locus_tag="ACHKBL_003124"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction histidine-protein
                     kinase/phosphatase UhpB"
                     /protein_id="HFO5819960.1"
                     /translation="MKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFP
                     FGLRLGLMLQCPRGYWPVLLGAEWLLIYWLTQAVGLTHFSLLMIGSLLTLLPVALISR
                     YRHQRDWRTLLLQGAALTAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFWH
                     YLANNTWLPLGPSLVSQPINWRGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLALP
                     IIALAWHYGWQGALIATLMNAIALIASQTWRDHPVDLLLSLLVQSLTGLLLGAGIQRL
                     RELNQSLQKELARNQHLAERLLETEESVRRDVARELHDDIGQTITAIRTQAGIVQRLA
                     ADNASVKQSGQLIEQLSLGVYDAVRRLLGRLRPRQLDDLTLEQAIRSLMREMELEGRG
                     IVSHLEWRIDESALSENQRVTLFRVCQEGLNNIVKHADASAVTLQGWQHDERLMLVIE
                     DDGSGLPPDSGQHGFGLTGMRERVTALGGTLHISCLHGTRVSVSLPQRYV"
     gene            complement(12105..12695)
                     /gene="uhpA"
                     /locus_tag="ACHKBL_003125"
     CDS             complement(12105..12695)
                     /gene="uhpA"
                     /locus_tag="ACHKBL_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator UhpA"
                     /protein_id="HFO5819961.1"
                     /translation="MITVALIDDHLIVRSGFAQLLGLEPDLQVVAEFGSGREALAGLP
                     GRGVQVCICDISMPDISGLELLSQLPKGMATIMLSVHDSPALVEQALNAGARGFLSKR
                     CSPDELIAAVHTVATGGCYLTPDIAIKLASGRQDPLTKRERQVAEKLAQGMAVKEIAA
                     ELGLSPKTVHVHRANLMEKLGVSNDVELARRMFDGW"
     gene            complement(12768..13058)
                     /gene="ilvN"
                     /locus_tag="ACHKBL_003126"
     CDS             complement(12768..13058)
                     /gene="ilvN"
                     /locus_tag="ACHKBL_003126"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HFO5819962.1"
                     /translation="MQNTTHDNVILELTVRNHPGVMTHVCGLFARRAFNVEGILCLPI
                     QDSDKSHIWLLVNDDQRLEQMISQIDKLEDVVKVQRNQSDPTMFNKIAVFFQ"
     gene            complement(13062..14750)
                     /gene="ilvB"
                     /locus_tag="ACHKBL_003127"
     CDS             complement(13062..14750)
                     /gene="ilvB"
                     /locus_tag="ACHKBL_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HFO5819963.1"
                     /translation="MASSGTTSTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYD
                     ALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDS
                     IPLICITGQVPASMIGTDAFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQS
                     GRPGPVWIDIPKDVQTAVFEIEAQPAVAEKAIAPAFSEESIRDAAAMINAAKRPVLYL
                     GGGVINAPARVRELAEKAQLPTTMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEA
                     DLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQL
                     IPLVEAQPRAEWHQLVADLQREFPCPIPKACDPLTHYGLINAVAACVDDSAIITTDVG
                     QHQMWTAQAYPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPDRKVLCFSGDGSLMMN
                     IQEMATASENQLDVKIILMNNEALGLVHQQQSLFYKQGVFAATYPGKINFMQIAAGFG
                     LETCDLNNETDPQAALQEIINRPGPALIHVRIDAEEKVYPMVPPGAANTEMVGE"
     gene            complement(14856..14954)
                     /gene="ivbL"
                     /locus_tag="ACHKBL_003128"
     CDS             complement(14856..14954)
                     /gene="ivbL"
                     /locus_tag="ACHKBL_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilvB operon leader peptide IvbL"
                     /protein_id="HFO5819964.1"
                     /translation="MTTSMLNAKLLPTAPSAAVVVVRVVVVVGNAP"
BASE COUNT         3871 a         4149 c         3735 g         3323 t
ORIGIN      
        1 actaaaactg attgattact ctactccctc accgtcatcg gtagtggtat ccttaacgta
       61 tattgatgaa aagacaaaac agtatgcaga gggaaaaatg ggttccacca gaaaggggat
      121 gctgaacgtt ctgattgccg ccgtgttgtg gggaagttca ggggtctgcg cgcaatacat
      181 catggagcaa agccagatgt cgtcgcagtt tttgactatg acgcgtttga tattcgccgg
      241 tttgattctg ctgacgctat catttgttca tggtgataaa atcttttcta ttattaacaa
      301 tcacaaagat gccattagcc tactgatttt ttccgtggtt ggcgcgctaa ccgtacagct
      361 cacttttttg ctaaccatag aaaaatcgaa cgcagccacg gcaacggtgc tgcaattcct
      421 ctcaccgact attatcgtcg cctggttctc actggtgcgt aaatcccgcc cgggcattct
      481 ggttttctgc gctattttga catcgctgat cgggactttt ttattggtga cacacggtaa
      541 tccgacgtca ttatcgatct ctcctgccgc gttattctgg ggcattgcct cggcatttgc
      601 tgctgcattc tataccacct atccctcaac gctgattgcc cgctatggca cgttaccggt
      661 cgtcggctgg agtatgctga ttggcggtct gatcctgctg cccttttatg ccagacaagg
      721 gacaaacttt gtcgttaacg gcagtttgat tctggcgttt ttttatttgg tggtcattgg
      781 tacgtccctg acatttagtc tgtacctgaa aggagcacaa ttaattggcg gtccaaaagc
      841 cagcatttta agctgtgcag aaccattaag tagcgcgctg ctctctttgc tgttgctggg
      901 gatcaccttc acattgccgg actggctggg aacgctgctg attctgtcat cggtgatttt
      961 gatttcaatg gattcccgcc gccgcgccag aaaaataaat cgtccggcgc ggcatgagtg
     1021 agaattacca gccaggcacc gcgcctccgt taaagatggt ttctgccgct ttagcgactt
     1081 cgggtgattg ataagattgc agaaattcct tcacgttttc tgcattctta ttatcttccc
     1141 gtgccaccag aatattcaca tacggcgaat ttttatcttc aataaatacg ctgtcgttaa
     1201 ccggagaaag tcctgtttgc tggatgtaag tggtgctgat aatcgctaca tccactttcg
     1261 ggtcgtccag tacacgtggc aactgtgccc cttccagctc cattatctgc aaatggcgtg
     1321 gattatcggt tatatccagc gcggtaggta gtaacccttt tccctctttc agagtaatta
     1381 gcttctcttt ttgcaacagt aaaagcgcgc gaccaaggtt ggtagggtcg ttggggatcg
     1441 ccactgtcgc gccttctttt atttgcgcca cggttttgat ttttttggaa taacccgcca
     1501 tggggaagac aaacgtattt cccaccgcta ccagtttata accatgcgcc tgattatctt
     1561 gttcaagaaa gggtcgatgc tggaagacgt tcgcatcaag ctcgccatga tttgtcgcat
     1621 cgttcggtag caacgaaccg ctaaatccta ccagttcaac atcgaggcca tatttctctt
     1681 ttgccacctt tttagcgact tccgcgacat cttgttctgc gccattaata acgcccactt
     1741 taatgtgctt ttcatcgctg ctgctctggt cgcaacctgc cagcagaatt cctgccagta
     1801 ataatgcggc ccccgcccgt agatgatgtg ttgtcagttt cattttttta tccttttatt
     1861 aatgattacc tgactatagc ggtaaggtcg ctgcagttta aaaaacgaaa cgctatcgat
     1921 aagaataaaa aggaataaaa gtggaatata aagtctggca cttcttactg accacgcaaa
     1981 cacctttcgt acagcatgat gagagcggca aaagcaaact acacttatgc ttcattcgat
     2041 gcacatttgc agaaggttgc cgctatgaag ccaacgatgc tactcatgat taccgtattc
     2101 ctgatttttc cagccatttc tcaggccgag tcgccattca gttctctaca gtcggcaaaa
     2161 gagaaaacca cggtattgca agatttacgt aaaatttgca cgccacaggc gtcattatca
     2221 gatgaagcgt gggaaaagtt aatgttgtca gatgaaaaca ataaacaaca tatacgcgag
     2281 gccatcgtgg cgatggaacg taataatcag agcaattatt gggaagcgtt gggtaaggta
     2341 gaatgcccgg atatgtaaga acggcaacaa ccactgacca acgcttcttt acctgatatc
     2401 aggatttctt cattttcacc tttgcagtca ccaacaatgc agtcagcaac atcaatgtgc
     2461 cggacaacat cagcggcgaa gtcagaccaa tgttatccaa cgcataaccg ccgattgccg
     2521 caccgcaggt attggcaagc tgaataacgg ccacctgaat agatccagcc ttttctgcct
     2581 gatcggccaa tgagcgagtg atccacgttg accaaccaac gggaattaaa gcaaaggtca
     2641 gcccccagat aatcgccaca ccagtagcaa caattttatc gcttccccac agcgtcagta
     2701 ccagcgcgct cagggcgagc acaaacggtg cgcccgctaa ggccagtttc accgaacgtt
     2761 tgagaataaa cgacgaaagc gatgtcccga caaagctggc gataccaaaa ctcagcagca
     2821 ccagggttaa gccatcaaca ccgaatcccg ccagattcat atacactggg cgaatataag
     2881 tgaaaaaagc aaactgtccg gcaaaggaca taaagatggc gatcatcccg gccatcactc
     2941 ccggacgttg taataagcgg aaagtatttt gtttctgatg cgaaggttca cctggcagcg
     3001 aaggcagtga tttgataatc cagaaaatac acagcacacc catcgccgcc gccgcattaa
     3061 agacattgcg ccaaccgata agctcgccta aaaaactgcc caacggcgcg gcaatcacca
     3121 gcgcaataga aaccgcgccg aagatcaccg acagcgcctt cggcaccgta cgcggcggta
     3181 ccagacgcat ggtcagcgac gccgacatcg cccagaaccc gcccagcgcc agccccagac
     3241 aggcgcgacc gattaacagc agactgaatg agttggcaaa ggaaaccagc aaacaggaaa
     3301 gcgtcagcaa aacggcgaac agaatgacaa cgtagcggcg gtcagtggcc tgaatggtct
     3361 gggtaataaa caaactggca aacatcgcca caaaggcggt cacggtcacc gattgcccgg
     3421 caaccccttc cgaaatgcct aaatcctggg ccattggcgt caacaaactg acaggcaaaa
     3481 actcaacgat aatcagacag gcgacgcaaa acgccaccga gaaaacggct gaccagttcg
     3541 gtcgggtgat ggcatccgcg ccgcggtttt cagcaataaa ttcactcatg gtgttacccg
     3601 tggcatgttt cagagggaaa gccgaccact ttaacattga aatgtgtgac gtatttaacg
     3661 tttttgcaac ttttacggtg ttgcttgagc tggagttaag atcgaaacgg aacagggcaa
     3721 agattaccgg atgcggcgtg aacgccttat ccaccctaca ggtcaggcgc ggtctgtagg
     3781 ctgataagac gaagcggttt taatgcagcc agtgaacgcc ctcggcaggc tgaaagaacg
     3841 cgttatagcg catctgaaaa gcattaatat cctgcatttc aggttcggtt tcgcgcagaa
     3901 aacccagcgc cagcctgacc tgagcatcgg taatgggagc ggcaaggcgg gctttttgta
     3961 aaatccgatg ccattgcagc aagttctgtt cgctaccatc gacaatgaca atttgccaga
     4021 tgagcaacac ttgcgcggcg ttttgcaggt gcgactcatc cggacgatgc aaataacgta
     4081 aaaattcgtc accggctgac tttcccattt gatcgaccat cgactccacg ggtacgacat
     4141 cgatgttcag ccgatcgctg agacggcgaa tcgcttttct ggccccggat gtcagcaccc
     4201 gaaaaccaac cacaaacact accgccagcg tggcgagcat tatccagatc atgagttctc
     4261 ctgcgtaatc agaactcatg atatctggaa accctcgcca gttactaatc caccagaata
     4321 atcttcagtg caaacagagc tgcgaccacc accacacaca ggttcagatc gcgccagcgc
     4381 ccggtgcaca ctttcatgat gcagtacgac ataaagccga gtgcaatccc ttcggtgatc
     4441 gagaaagtaa agggcatcat caccgtggta ataaacgcag gtaccgattc ggtaaaatca
     4501 tcccagttaa cgcgcgccag acttgaggtc atcaacacgc caacaaagat taacgctccg
     4561 gcggttgcgt aaggaggaac catcgccacc agcggtgaga agaacataac caacaggaac
     4621 ataacgccga ccacaaccgc agtcagcccc gtgcgaccac cgactgccac accagaagta
     4681 ctttcaatat aggcggtaac agacgaggtg ccgataaacg cacccgccac cgaactgacg
     4741 ctatcaacat acagcgcctt attcatatta gggaatttac cgttactatc tattaagcca
     4801 gctttatcag ttacaccaat taatgttcct gatgaatcaa ataggttgat cagcataaag
     4861 gagaaaatga taccggcgag ttcaagtgat aacgcgccgc tcaaatccac ttcaccaatg
     4921 acgctgctaa tatcaggcgg aatggaatag acgccgctaa aatgaacatc accgaaaaat
     4981 aatccacagc aagacgtcac cacaatagaa acaagcaccg cggcatgaaa atgacgtgat
     5041 gacaacacgg ttataataaa aaaccctaaa atacctaaca acacgccgtg agaacttaaa
     5101 tcgccaatca tcaccagggt gtctttattg gcgacaataa cgccagtatt ttttaatccc
     5161 attaaggcga taaataatcc gattccgctg gtgataccaa tacgtaaact taatggaatg
     5221 ttggagatca tccagtaccg gatacgaaac agcgtgagca gaaatagccc aactgcgccc
     5281 cagaatatcg cgcccatccc ggtctgccag gagatgccca tcgcccccac gaccacgaac
     5341 gcaaagaagg cgttcagccc cattgccgga gccagcgcca cgggtaagtt agcaaatatc
     5401 cccatcgcaa tactgccgat accggcaatc aaacaggtgg taacaaacac cactttcggg
     5461 tccatttgtg ccgcgccgag gatttgcgga ttcacaaaaa cgatgtatac catggttaaa
     5521 aaagtggtca tccccgcaat caattctgtg cggacggtgg tcccatgctg aggtagttta
     5581 aataatcgcg aaagcgtccc tgattcatta ctcacgtaat cggtattgtc attattcatt
     5641 tttttgtcca ttggaggaga tttaatccct tgcttttgta aaacaaatga caacaacggt
     5701 tcagtgataa ttatcacatt tcaattgcac attaatggat attctttaat aatctcgcga
     5761 cgtttcttta tgataaataa taatcaaatt gataaaatca aaatgagaaa aatatgaata
     5821 attctattaa ccataaattt catcacatta gccgggctga ataccaggaa ttgttagccg
     5881 tttcccgtgg agacgctgtt gccgattata ttattgataa tgtctctatt ctcgacctga
     5941 tcaatggcgg agaaatttcc ggcccaattg tgattaaagg acgttacatt gccggtgttg
     6001 gcgcagaata cgctgatgct ccggctttgc agcggattga tgcccgcggc gcaacggcgg
     6061 tgccagggtt tattgatgcg cacctgcata ttgaatccag catgatgacg ccggtcacct
     6121 ttgaaaccgc taccctgccg cgcggcctga cgaccgttat ttgcgaccct catgaaatcg
     6181 tcaacgtgat gggcgaagcc ggtttcgcct ggtttgcccg ctgtgccgaa caggcaaggc
     6241 aaaaccagta cttacaggtc agctcttgcg tacccgccct ggaaggctgc gatgttaacg
     6301 gtgccagttt tacccttgaa cagatgctcg cctggcggga ccatccgcag gttaccggcc
     6361 ttgcagaaat gatggactat cctggcgtaa ttagcgggca gaatgcgctg ctcgataaac
     6421 tggacgcatt tcgccacctg acgctggacg gtcactgccc gggtttgggt ggtaaagaac
     6481 ttaacaccta tattgctgcg ggtattgaaa actgccacga aagttatcag ctggaagaag
     6541 gacgccggaa attacaactc ggtatgtcgt tgatgatccg cgaagggtcc gcagcccgca
     6601 atctcaacgc gctggcaccg ttgatcaacg aatttaacag cccgcaatgc atgctctgta
     6661 ccgatgaccg taacccgtgg gagatcgccc atgaaggaca tatcgatgcc ttaattcgcc
     6721 gcctgatcga acaacacaat gtgccgctgc atgtggcata tcgcgtcgcc agctggtcga
     6781 cggcgcgcca ctttggtctg aatcacctcg gcttactggc acccggtaag caggccgata
     6841 tcgtcctgtt gagcgatgcg cgtaaggtca cggtgcaaca ggtactggtg aaaggcgagc
     6901 cgattgatgc gcaaacctta caggcggaag agtcggcgag actggcacaa tccgccccgc
     6961 catatggcaa taccatttcc cgccagccag tttccgccag cgactttgcc ctgcaattta
     7021 cccccggaaa acgctatcgc gttattgagg tcatccataa cgaattgatt acccactccc
     7081 gctccagcgt ctacagcgaa aatggttttg atcgcgatga tgtgtgcttt attgccgtac
     7141 ttgagcgtta cgggcaacgg ctggctccgg cctgtggttt gctcggcggc tttggcctga
     7201 atgaaggtgc gctggcggcg acggtcagcc atgacagcca taatattgtg gtgatcggtc
     7261 gtagcgcaga agagatggcg ctggcggtca atcaggtgat tcaggatggc ggcgggctgt
     7321 gcgtgatccg taacggtcag gtacaaagtc atctaccgtt gcccattgcc gggctaatga
     7381 gcaccgacac ggcgcagtca ctggcggagc aaattgacgc cttgaaagcc gccgcccgtg
     7441 aatgcggtcc gttaccggat gagccgttta ttcagatggc gtttctttcc ctgccagtga
     7501 tccccgcgct gaaactaacc agtcaggggc tgtttgatgg cgagaagttt gccttcacta
     7561 cactggaagt cacggaataa taaaaaaaag cccggcgtca tgccgggcaa aagtcaccag
     7621 ttaagtttat gccactgtca actgctgaat ttttttctcg cggcggattt tgcgttcttc
     7681 catcaccgcc actatcgcca tcaggcagat acaaccaatc gcggcgatat ccagcgcggc
     7741 gaaggtgcct gcccagccgg taaggccaaa taccggcgtc ccatcggcaa tcattcccag
     7801 acctaacttg gcaaagctgt caccaatcag gtaagcgaag gtgcctttaa taccatcggc
     7861 agcgccaatc gcttttttag gtacaaagcc aacagcagcc acaccaatca acaattgcgg
     7921 gccaaagacc aggaaaccca acgcaaagag agaagccagg tagatatatt cgttgctggc
     7981 gtgttgatac acaccgagcg tggcgataat cagcgccagc gcgatacagg ccaccaggcc
     8041 acggcgaccg ttcgccaggt cagagagcca gccccacagc agcgtaccga ccagcgcacc
     8101 agcttcaaac agcgtaaagc tctgaatcgc caccgcttta gagagtttca gttcctggaa
     8161 cgcatacacg gttgaccact ggtcgatacc aatacgcacc acatagagga aaatgttggc
     8221 gaagcacagc agccagatca ctttgttttt cagcacatac tcaacaaaga tctgccactt
     8281 ggtcatatcg gtagattctg tctctttgtc ctcttcgctg atctcctcgc cgaacagctc
     8341 ttcagctttg ccgaggccat aagattccgg ggagtcgctg ccgtaacgca ggccgataaa
     8401 accgacaatc agcgcgataa tcgacgggaa aataaacatg ccgatgacat gaccatcgaa
     8461 caggtaattt gcgccgaaca gcgccacacc tgctgcgcct gcaccaccaa ggttgtgaga
     8521 aatgttccag aaaccgagga atgtcccgcg tttacgacgc ggcgtccatt tggtgatggt
     8581 ggagtaactg cacgaaccgc cggtactctg gaaaaagccg ctcaaggcgt agaaggcaat
     8641 catcaggaac aggctaaccg agccgctgcc catactggca ctgaagccca gcatacaaat
     8701 ggcggacagg atcagcatga acggcaggaa ttgtttggtg tttttgccgt cggcgtagta
     8761 agaaaccagc gttttaccca cgccataagt gatggagaag cccaggccga tcatccccag
     8821 ctgcgtcatg ctcaacccgt aggtcgagat catgtcgttc tgcgcgatgt taaagttctt
     8881 gcgaatcagg tacatcgtaa ggtagccgat aaagaccacc aggtaggatt gcatgaacgg
     8941 tttgaaccac attttgcgcc gcacttcgag cggaaggtcc agggtcggct tgcgaacctg
     9001 gtttaggaaa gccagcatgg gttactcctg atttcccttc atacttcaag ccgcaggtgc
     9061 cttagctgca cgcattcctc ccggtcacat agttcactat gctcccggga ctcacgcgtt
     9121 tgctgcgttc ctgcaacttg aattattttg ggaaagaatt aatgaatgat ggcattgtaa
     9181 aaatcagcag caaatcaggc ctgagatagc gtccaggcaa aacctggaaa attgcttagt
     9241 tttgccggat atgaagtgaa aaggtgatat gcatcacgct tcgcgcggtg tctgggcgtt
     9301 caaaaagggc agtaacagca gtgcggaaac cccggcggca atggcgataa ccacaaaaaa
     9361 tccgctccag tgccaggtat cgagcacttt cgccagcggc caaccagcaa gcgacgcccc
     9421 cagataagca aacaagccga caaaccccgt cgccgcccct gccgcctctt tgtgggaaca
     9481 ttctgccgcc gccatgccga ttaacatctg cgggccaaag acaaaaaaac caatggtgaa
     9541 gaagcaggtt gcctgcatca cgtagctggc aaatggcatc agccacagcg agccgactga
     9601 aagcaaaatt ccggcggcga aaatcaaatt catcggcccg cggttgccgt taaacaattt
     9661 gtccgagccc caacccgcta ccagcgcacc gataaatcca cccagttcaa acatcgtcac
     9721 tgccgtattc gccgtgacca gatcgacgcc cagcgtctcg gacatataca aattgcccca
     9781 gtcgttgatt gccgcccgga ccacatagac cagcacatag caaaacgaaa gcagccagat
     9841 atacggattc agtaacacat atttggtgag gatctcttta cgcgtcagcc ctgccccttc
     9901 ttgttgttga gctatctcca gcgcgtcgtg ccgccattca ccaaccgccg gtaaacctaa
     9961 cgcctgcggg cgatcgcgca gccgccagca gagaaaaatc cccacgacta tcgccataca
    10021 acccgcaatc atcatcccgg cacgccagcc gtaatgcagc gcgctcgctg ccatcacaat
    10081 gggaatgagt gcgccgccga cgttatgcgc cgtgttccat aatgcccacc aaccgccgcg
    10141 ctcggtacga gaataccagg cggttaacag acgcgcacac accggtgaac cccagccctg
    10201 gaaaaaggcg ttcaacaccc aaagcacggc aaacgcccat agcgacgtcg agaagccaaa
    10261 cagaatgttg ataatgcccg tggcaataag ccctatcccc ataaaataac gggcatttga
    10321 gcgatcgctg acaatgccgg agacaaactt cgacacgccg taggtaatgt aaaacagggt
    10381 cgctaacagg ccaatatcgc tacggctgag cacgccgtta gcaaggattt ctggtacggc
    10441 ggcgttaaaa cttttacgcg tgaagtaaaa cagcgcgtaa cccagccaga tggtcagcag
    10501 aatatgccga cgccagtagc gatagcgggc atcaatttca tgtttatcag tcagtaatgg
    10561 cgcatcggca ggcgctttca gaaacggcaa catcatcgaa ccttagacat aacgttgagg
    10621 tagagaaacg ctgacacgcg tgccgtgcag acaggaaatg tgtaatgtgc cacccagcgc
    10681 cgttacgcgc tcgcgcattc cggtaaggcc aaaaccgtgt tgcccggaat ctggcggtaa
    10741 accgctgcca tcgtcttcaa tcaccagcat taaccgctca tcatgctgcc agccttgcag
    10801 cgtgaccgcg ctggcatcgg catgtttcac aatgttgttc aacccttcct ggcagacgcg
    10861 aaatagcgtc acgcgctggt tttcgcttaa cgctgattca tcgattcgcc attcgagatg
    10921 actgacaatg ccgcgccctt ccagctccat ttcccgcatc agtgagcgga tggcctgctc
    10981 cagggtgaga tcatccagct ggcgcggacg taaccgccct aacaagcggc gcaccgcgtc
    11041 gtaaacgccc agcgatagtt gttcgatgag ctgcccgctc tgtttcacgc tggcgttatc
    11101 cgccgcgagc cgctggacaa tgcccgcttg agttcgaata gcagtaatgg tctgaccgat
    11161 atcatcgtgc agctcacgtg ccacatcacg gcgcacgctc tcttcggttt ccagcaaccg
    11221 ttcagcgaga tgctgattgc gcgccagttc cttttgcagc gactggttaa gttcacgcaa
    11281 ccgctggatg cctgcgccca gtaacaaccc tgtcagactt tgcaccagca gcgagagcaa
    11341 taaatccacc ggatgatcgc gccaggtttg actggcgatc agcgcgatgg cgttcatcaa
    11401 cgtcgcaatt agcgcccctt gccagccata gtgccaggcc agcgcgataa tcggcagcgc
    11461 caggcagaat ggcgtaaagc gcgacagttc gtccggcaat cccaactgga gccagagact
    11521 gataacaaac agcagcaagt accagaccag atgccgccca cgccagttga tcggttgaga
    11581 aactaacgac gggccgagcg gcagccaggt gttattggcg agatagtgcc agaacaccag
    11641 acatatcggg gccagcgtca ggccgccagt taaagtcagc aacagcgcat tccacgactc
    11701 tttgccgtgc caaagccagg gcagcgactg caacaacgcc gccgccgtta atgccgcccc
    11761 ttgtagcagc aaggtgcgcc agtcacgttg atgtcgatag cgcgagatca gcgccacggg
    11821 cagtaacgtc agtaaactac cgatcatcaa taaggagaaa tgggttaaac cgaccgcctg
    11881 cgttaaccag taaatcagca gccactccgc gcccagcaac accggccagt atccgcgcgg
    11941 gcattgcagc attagcccca gacgcagacc aaacggaaat aacagcaccg ccatatcagg
    12001 gcgctcaacc aggtgcaggc tgatactcca caggcaaaac catgcggcag agaagataaa
    12061 aaagcaggca ataacggtaa ttaagcggga gaacaacgtc ttcatcacca accatcaaac
    12121 atgcggcgcg ccagttcaac gtcgttactg acgcccagtt tttccatcag attggcgcga
    12181 tggacgtgta ccgttttcgg cgacaagccc agttcggcgg caatctcttt caccgccatt
    12241 ccttgcgcca gtttttctgc cacctggcgt tcgcgtttgg ttagcgggtc ctgacgaccg
    12301 gatgccagtt taatggcaat atccggcgtc agataacagc cgcccgtggc aaccgtatgc
    12361 accgcagcaa tcagttcgtc agggctacaa cgcttggaga gaaagccgcg cgcccccgcg
    12421 ttaagcgcct gctcaaccag cgccggactg tcatgaacag agagcataat cgtcgccata
    12481 cctttcggca gctggcttag cagctccaga ccggagatat cgggcatgga gatatcgcaa
    12541 atacacacct gcacaccgcg ccccggcaac cccgccagcg cctcgcgccc cgaaccaaac
    12601 tcggcaacta cctgcaaatc aggttccagc cccagcagct gcgcaaagcc ggagcggacg
    12661 ataaggtgat cgtctataag ggcaacggtg atcatgatct tgtcctggcg ggtaataaaa
    12721 cgcgcttacc ttaacgataa gcgcgatgtt gttcaagcct tgagcggtta ctgaaaaaac
    12781 accgcaatct tgttaaacat tgtcggatcg gactgattac gctgcacttt caccacatct
    12841 tccagcttat cgatttggct tatcatctgc tccagacgct ggtcgtcatt gaccagtagc
    12901 cagatatggc ttttgtcgct gtcctgaatc ggcaggcaga gaatgccttc aacgttaaaa
    12961 gcgcgacggg caaaaaggcc acaaacgtgg gtcattacgc ccggatggtt gcgaacggtg
    13021 agttcgagaa tcacgttgtc atgagttgtg ttttgcatgg cttattcccc caccatttca
    13081 gtattcgccg cacctggcgg caccatcggg tacacttttt cttcggcatc aatgcgcacg
    13141 tggatcagcg ccggaccagg gcgattgatg atttcctgca atgcagcctg cggatcggtt
    13201 tcgttattca aatcacaggt ttcgaggccg aatccggcgg caatctgcat aaagttgatt
    13261 tttcccggat aggtagcggc aaaaacgccc tgcttgtaga acagactctg ttgctgatgt
    13321 accagcccca gcgcttcgtt gttcatcaga atgattttga catccagctg attttcgcta
    13381 gcggtcgcca tctcctgaat attcatcatc aggctgccgt cgccggagaa acacaacact
    13441 ttgcgatccg ggttcgcaag cgctgcgcca atcgccgcag gcaggccaaa gcccatcgtg
    13501 cccagcccac cggaggtcag ccactggcgt gggcgattga gcggataagc ctgcgccgtc
    13561 cacatctgat gctgacccac gtcggtggtg ataatcgcgc tgtcatcgac acaggcggca
    13621 acagcgttga tcaggccgta atgcgttaac ggatcgcacg ctttcgggat tggacacggg
    13681 aattcacgct gcaaatccgc caccaactgg tgccactctg cacgcggttg cgcttccacc
    13741 agcgggatca actgcgccaa cacgtcatca acatccgcct gaattgccac atgcggttgc
    13801 ttgattttgc ccagctctgc gcggtcgata tcgacatgaa taattttggc attcgggcag
    13861 aactgctcgg ttttgccaat cgcccggtca tcaaaacgcg cgccgagaac aatcagcaga
    13921 tccgcctcct gcaaaatata gttggtgctg cgcacgccgt gcatccctag catacccagc
    13981 gacagcggat gcgcttttgg caacataccc agcgccatta atgtcattgt ggtaggcagc
    14041 tgcgctttct ccgccagttc acgcactctc gcaggcgcat tgatcacacc accgcccaga
    14101 taaagcaccg ggcgtttggc agcattaatc attgccgctg cgtcacgaat gctttcttcg
    14161 ctaaaggcgg gcgcgatggc tttttctgcc accgcgggct gtgcttcaat ctcaaaaacg
    14221 gccgtttgca catccttagg aatgtctatc cacaccgggc cagggcggcc tgattgcgca
    14281 atacggaagg catcgctcat gacctgcggg agttcttcga tatgtctgac cagatagttg
    14341 tgtttggtga tggggataga gatgccgtag gtgtcgactt cctggaaggc gtcggtgccg
    14401 atcatcgagg cgggaacctg accagtgatg caaatcagcg ggatggagtc cagccgcgca
    14461 tcggcaatgg cggtcaccag gttagtcgcc cccggtccgc tacaggccat acagaccgcc
    14521 ggtttaccgt cggtgcgcgc cattccctga gcgataaacc ccgcgccctg ttcatggcga
    14581 gccagaatat ggcggatttg cgtgctttgg cttaaggcat cgtaaacagg caggatagaa
    14641 ccgcccggaa tgcccgtcac aatcttaatg ccctgctgtt ccaggaaatg aacgataaat
    14701 tctgcgccgg taaagcgctt acgcgtcgat gttgtgcccg aacttgccat gctccagtcc
    14761 ttttcttctg ggccgacttt ccgggaggtg cttaaacgaa aaaccccgcc cggtttgcgc
    14821 cggcggggtt ttggaatcgt gtgttgttcc agtccctacg gcgcattgcc gacgaccacc
    14881 accacacgca cgacgaccac tgcggcggat ggcgcagttg gtagtagttt tgcgttgagc
    14941 atggaagtag tcattgggga ccttgttggt tttgtgttta acaatattta tacaagcaca
    15001 gctttacagg ggagacaatg gaaaattttt cagcaaggga aaattgaggg gttgatcacg
    15061 ttttgtactg aattgcag
//
LOCUS       DAZFLD010000121        15059 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.122, whole genome shotgun sequence.
ACCESSION   DAZFLD010000121 DAZFLD010000000
VERSION     DAZFLD010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15059
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.122"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            4..80
                     /locus_tag="ACHKBL_003129"
     tRNA            4..80
                     /locus_tag="ACHKBL_003129"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:38..40,aa:Arg,seq:acg)
     gene            361..927
                     /gene="yqaB"
                     /locus_tag="ACHKBL_003130"
     CDS             361..927
                     /gene="yqaB"
                     /locus_tag="ACHKBL_003130"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1-phosphate/6-phosphogluconate
                     phosphatase"
                     /protein_id="HFO5819965.1"
                     /translation="MYERYAGLIFDMDGTILDTEPTHRKAWREVLGHYGLQYDVQAMI
                     ALNGSPTWRIAQAIIELNQADLDPHALAREKTEAVRSMLLDSVEPLPLVEVVKSWHGR
                     RPMAVGTGSESAIAEALLAHLGLRRYFDAVVAADHVKHHKPAPDTFLLCAQRMGVQPT
                     QCVVFEDADFGIQAARAAGMDAVDVRLL"
     gene            924..1352
                     /locus_tag="ACHKBL_003131"
     CDS             924..1352
                     /locus_tag="ACHKBL_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaA family protein"
                     /protein_id="HFO5819966.1"
                     /translation="MSEALSLFSLFASSFLSATLLPGNSEVVLVAMLLSGISHPWVLV
                     LTATMGNSLGGLTNVILGRFFPLRKTSRWQEKATGWLKRYGAVTLLLSWMPVVGDLLC
                     LLAGWMRISWGPVIFFLCLGKALRYVAVAAATVQGMMWWH"
     gene            1425..2981
                     /gene="gshA"
                     /locus_tag="ACHKBL_003132"
     CDS             1425..2981
                     /gene="gshA"
                     /locus_tag="ACHKBL_003132"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HFO5819967.1"
                     /translation="MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTLATTGH
                     PEALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPL
                     SMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFW
                     QAKCGDISGADAKEKISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPF
                     EKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAK
                     IGIEKDGKRLQINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDI
                     NPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSSELACTRVNWNRVILEGRKPGLTLG
                     IGCETAQFPLPQVGKDLFRDLKRVAQTLDSINGGEAYQKVCDELVACFDNPDLTFSAR
                     ILRSMIDTGIGGTGKAFAEAYRNLLREEPLEILREEDFIAEREASERRQQEMEAADTE
                     PFAVWLEKHA"
     gene            3131..3646
                     /gene="luxS"
                     /locus_tag="ACHKBL_003133"
     CDS             3131..3646
                     /gene="luxS"
                     /locus_tag="ACHKBL_003133"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008458247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HFO5819968.1"
                     /translation="MPLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPN
                     KEVMPERGIHTLEHLFAGFMRNHLNGNGVEIIDISPMGCRTGFYMSLIGTPDEQRVAD
                     AWKAAMEDVLKVQDQNQIPELNVYQCGTYQMHSLQEAQDIARSILERDVRINSNEELA
                     LPKEKLQELHI"
     gene            complement(3697..4809)
                     /locus_tag="ACHKBL_003134"
     CDS             complement(3697..4809)
                     /locus_tag="ACHKBL_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFO5819969.1"
                     /translation="MIWQLTDDKRWSALRQRFSWVEEMHHTPQDPEHHGEGDVGVHTE
                     MVLNALVTQPEFQQLPAQQQEVLWAAALLHDVEKRSTTVQENGRIQSPGHARRGELTA
                     RQILWRDIPTPFVLREQIVALVRLHGLPLWLLERPEPERLLLTAAMRIDTRLLALLAR
                     ADLLGRQSPDQQSMLERIDLFELFCHEQQCWGKMRPFVSDSARWHYLTHEQSSPDFVP
                     WEAEPFEVILLCGLPGMGKDRYISEQCQGMDVISLDDMRRRINASPDDKTATGRIVQQ
                     AKEEARVFLRQKKPFIWNATNITRQLRSQLISLFTAYGARVKIVYLEVPWAQWKQQNA
                     RREYAVPEAVVMRMASRLEVPQPDEAHSVEYRMTDR"
     gene            complement(4806..5513)
                     /locus_tag="ACHKBL_003135"
     CDS             complement(4806..5513)
                     /locus_tag="ACHKBL_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001613653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase family protein"
                     /protein_id="HFO5819970.1"
                     /translation="MNTQRKYGRTWHYPFSPGTTSDDRINADYWQDLQAITQLVHTEK
                     LDGENNCLNRYGVFARSHAAPTQSAWTYKIRQRWQLLKNDLGDLELFGENLYAVHSIE
                     YRALEQDFYLFAVRCQDMWLSWEEVQFYAALFDFPCVPEISGPQPGNDEKSWQRDFLA
                     LTNARGAFDPWDTQTGQPCTLEGIVSRNRDAFSVADFSHNVFKYVRKNHVKTTVHWKR
                     HWQRARMAHEFDYGEQS"
     gene            complement(5771..7309)
                     /gene="emrB"
                     /locus_tag="ACHKBL_003136"
     CDS             complement(5771..7309)
                     /gene="emrB"
                     /locus_tag="ACHKBL_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrB"
                     /protein_id="HFO5819971.1"
                     /translation="MQQQKPLEGAQLVIMTIALSLATFMQVLDSTIANVAIPTIAGNL
                     GSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWACGVSSSL
                     NMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
                     ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTERRRIDAVGLALLVIGIGSLQI
                     MLDRGKELDWFSSQEIIILTVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLC
                     ISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVILSPIIGRFAHKLDMR
                     RLVTFSFIMYAVCFYWRAYTFEPGMDFGASAWPQFIQGFAVACFFMPLTTITLSGLPP
                     ERLAAASSLSNFTRTLAGSIGTSITTTMWTNRESMHHAQLTESVNPFNPNAQAMYSQL
                     EGLGMTQQQASGWIAQQITNQGLIISANEIFWMSAGIFLVLLGLVWFAKPPFGAGGGG
                     GGAH"
     gene            complement(7326..8498)
                     /gene="emrA"
                     /locus_tag="ACHKBL_003137"
     CDS             complement(7326..8498)
                     /gene="emrA"
                     /locus_tag="ACHKBL_003137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrA"
                     /protein_id="HFO5819972.1"
                     /translation="MSANAETQTPQQPVKKSGKRKRLLLLLTLLFIIIAVAIGIYWFL
                     VLRHFEETDDAYVAGNQIQIMSQVSGSVTKVWADNTDFVKEGDVLVTLDPTDARQAFE
                     KAKTALASSVRQTHQLMINSKQLQANIEVQKIALAKAQSDYNRRVPLGNANLIGREEL
                     QHARDAVTSAQAQLDVAIQQYNANQAMILGTKLEDQPAVQQAATEVRNAWLALERTRI
                     VSPMTGYVSRRAVQPGAQISPTTPLMAVVPATNMWVDANFKETQIANMRIGQPVTITT
                     DIYGDDVKYTGKVVGLDMGTGSAFSLLPAQNATGNWIKVVQRLPVRIELDQKQLEQYP
                     LRIGLSTLVSVNTTNRDGQVLANKVRSTPVAVSTAREISLAPVNKLIDDIVKANAG"
     gene            complement(8625..9155)
                     /gene="emrR"
                     /locus_tag="ACHKBL_003138"
     CDS             complement(8625..9155)
                     /gene="emrR"
                     /locus_tag="ACHKBL_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter EmrAB
                     transcriptional repressor EmrR"
                     /protein_id="HFO5819973.1"
                     /translation="MDSSFTPIEQMLKFRASRHEDFPYQEILLTRLCMHMQSKLLENR
                     NKMLKAQGINETLFMALITLESQENHSIQPSELSCALGSSRTNATRIADELEKRGWIE
                     RRESDNDRRCLHLQLTEKGHEFLREVLPPQHNCLHQLWSALSTTEKDQLEQITRKLLS
                     RLDQMEQDGVVLEAMS"
     gene            complement(9246..9581)
                     /gene="ygaH"
                     /locus_tag="ACHKBL_003139"
     CDS             complement(9246..9581)
                     /gene="ygaH"
                     /locus_tag="ACHKBL_003139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine transporter subunit YgaH"
                     /protein_id="HFO5819974.1"
                     /translation="MSYEVLLLGLLVGAANYCFRYLPLRLRVGNARPTKRGAVGILLD
                     TIGIASICALLVVSTAPEVMHDTRRFVPTLVGFAVLGASFYKTRSIIIPTLLSALAYG
                     LAWKVMAII"
     gene            complement(9571..10308)
                     /gene="ygaZ"
                     /locus_tag="ACHKBL_003140"
     CDS             complement(9571..10308)
                     /gene="ygaZ"
                     /locus_tag="ACHKBL_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine exporter subunit YgaZ"
                     /protein_id="HFO5819975.1"
                     /translation="MESPTPLPAPGSATFMEGCKDSLPIVISYIPVAFAFGLNATRLG
                     FSPLESVFFSCIIYAGASQFVITAMLAAGSSLWVAALTVMAMDVRHVLYGPSLRSRII
                     QRLQKSKTALWAFGLTDEVFAAATAKLVRNNRRWSENWMIGIAFSSWSSWVFGTVIGA
                     FSGSGLLQGYPAVEAALGFMLPALFMSFLLASFQRKQSLCVTAALVGALAGVTLFSIP
                     VAILAGIVCGCLTALIQAFWQGAPDEL"
     gene            complement(10432..11616)
                     /locus_tag="ACHKBL_003141"
     CDS             complement(10432..11616)
                     /locus_tag="ACHKBL_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO5819976.1"
                     /translation="MTKPNHELSPALIVLMSIATGLAVASNYYAQPLLDTIARNFSLS
                     ASSAGFIVTAAQLGYAAGLLFLVPLGDMFERRRLIVSMTLLAACGMLITASSQSLAMM
                     ILGTALTGLFSVVAQILVPLAATLASPDKRGKVVGTIMSGLLLGILLARTVAGLLANL
                     GGWRTVFWVASVLMALMALALWRGLPQMKSETHLNYPQLLGSVFSMFISDKILRTRTL
                     LGCLTFANFSILWTSMAFLLAAPPFNYSDGVIGLFGLAGAAGALGARPAGGFADKGKS
                     HHTTTFGLLLLLLPWLAIWFGHTSVLALIIGILVLDLTVQGVHITNQTVIYRIHPDAR
                     NRLTAGYMTSYFIGGAAGSLISASVWQHGGWAGVCLAGATIALVNLLVWWRGFHRQEA
                     AI"
     gene            complement(11808..12800)
                     /gene="proX"
                     /locus_tag="ACHKBL_003142"
     CDS             complement(11808..12800)
                     /gene="proX"
                     /locus_tag="ACHKBL_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="HFO5819977.1"
                     /translation="MRHSVLFATAFATLISTQTFAADLPGKGITVNPVQSTITEETFQ
                     TLLVSRALEKLGYTVNKPSEVDYNVGYTSLASGDATFTAVNWTPLHDNMYEAAGGDKK
                     FYREGVFVNGAAQGYLIDKKTADQYKITNIAQLKDPKIAKLFDTNGDGKADLTGCNPG
                     WGCEGAINHQLAAYGLTNTVTHNQGNYAAMMADTISRYKEGKPVFYYTWTPYWVSNEL
                     KPGKDVVWLQVPFSALPGDKNADTKLPNGANYGFPVSTMHIVANKAWAEKNPAAAKLF
                     AIMQLPVADINAQNAIMHDGKASEGDIQGHVDGWIKAHQQQFDGWVNEALAAQK"
     gene            complement(12858..13922)
                     /gene="proW"
                     /locus_tag="ACHKBL_003143"
     CDS             complement(12858..13922)
                     /gene="proW"
                     /locus_tag="ACHKBL_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     permease ProW"
                     /protein_id="HFO5819978.1"
                     /translation="MADQNNPWDTTPAADSAAQSADAWGTPAAAPTDGGGADWLTSTP
                     APNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFRPVFQGVRVPVDYILNGFQQLLL
                     GMPAPVAIIVFALIAWQISGVGMGVATLVSLIAIGAIGAWSQAMVTLALVLTALLFCI
                     VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFA
                     LPPIIRLTILGINQVPADLIEASRSFGASPRQMLFKVQLPLAMPTIMAGVNQTLMLAL
                     SMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIILDRLTQAVGRDSRSR
                     GNRRWYTTGPVGLLTRPFIK"
     gene            complement(13915..>15059)
                     /gene="proV"
                     /locus_tag="ACHKBL_003144"
     CDS             complement(13915..>15059)
                     /gene="proV"
                     /locus_tag="ACHKBL_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0031460 - glycine betaine transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein ProV"
                     /protein_id="HFO5819979.1"
                     /translation="AFKYIEQGLSKEQILEKTGLSLGVKDASLAIEEGEIFVIMGLSG
                     SGKSTMVRLLNRLIEPTRGQVLIDGVDIAKISDAELREVRRKKIAMVFQSFALMPHMT
                     VLDNTAFGMELAGINAEERREKALDALRQVGLENYAHSYPDELSGGMRQRVGLARALA
                     INPDILLMDEAFSALDPLIRTEMQDELVKLQAKHQRTIVFISHDLDEAMRIGDRIAIM
                     QNGEVVQVGTPDEILNNPANDYVRTFFRGVDISQVFSAKDIARRTPNGLIRKTPGFGP
                     RSALKLLQDEDREYGYVIERGNKFVGAVSIDSLKAALTQQQGLDAALIDAPLAVDAQT
                     PLSELLSHVGQAPCAVPVVDEDQQYVGIISKGMLLRALDREGVNNG"
BASE COUNT         3699 a         3903 c         4011 g         3446 t
ORIGIN      
        1 aatgcatccg tagctcagct ggatagagta ctcggctacg aaccgagcgg tcggaggttc
       61 gaatcctccc ggatgcacca tattctccgt aaccttcagc aatgaaggta tcgaagaggt
      121 agcggtatta accgcaggca ccagggagga taacgttgcg ccagcagcgg cccgaagggc
      181 gaacgccagt gagtcatcct cccggatgca ccatcttctt caaaaaattt ccttcaaaaa
      241 tatctcattt ctaaataact actttatcca tttcctgaat attcttctcc ttctccatca
      301 gcgacaaaat caagcaatta cgctaaagta gcgtcatatt tttcgacttg cgagaagacc
      361 atgtacgagc gttatgcagg tttaattttt gatatggatg gcacaatcct ggatacggag
      421 cctacgcacc gtaaagcgtg gcgcgaagta ttagggcact acggtcttca gtacgatgtt
      481 caggcgatga ttgcgcttaa tggctcgccc acctggcgta ttgctcaggc gattattgag
      541 ctgaatcagg ccgatctcga cccgcatgcg ttagcgcgtg aaaaaacaga agcagtaaga
      601 agtatgctgc tggatagtgt cgagccgctt cctcttgttg aagtggtgaa aagctggcat
      661 ggtcgtcgcc caatggctgt aggaacgggg agtgaaagcg ccatcgctga ggcattgctg
      721 gcacacctgg gattacgccg ttattttgac gccgtcgtcg ctgccgatca cgtcaaacac
      781 cataaacccg cgccagacac atttttgttg tgcgcgcagc gtatgggcgt gcaaccgacg
      841 cagtgtgtgg tcttcgaaga tgccgatttc ggtattcagg cggcccgtgc agcaggcatg
      901 gacgccgtgg atgttcgctt gctgtgagtg aagcgttatc gcttttctcg ttgtttgcca
      961 gtagttttct cagcgctaca ctattacccg gcaactccga agtcgtgctg gtggcaatgt
     1021 tgctttccgg gatcagtcat ccctgggttt tagttttaac agcaacaatg ggtaatagcc
     1081 ttggagggtt aactaacgtt atccttgggc gtttctttcc attgcgtaaa acatcgcgct
     1141 ggcaagagaa agctaccggc tggctgaaac gctatggtgc agtcacgcta ttattaagct
     1201 ggatgcccgt ggttggcgat ttactgtgtc tgttagcggg atggatgcgc atctcgtggg
     1261 gaccggtaat cttttttttg tgccttggta aagcgttacg ctatgttgcg gttgcagcag
     1321 ctaccgttca gggcatgatg tggtggcact aattgtaggc ctgcacatat ggtcaccatt
     1381 acagttatgc taattaaaac gattttgaca ggcgggaggt caatttgatc ccggacgtat
     1441 cacaggcgct ggcctggctg gaaaaacatc ctcaggcgtt aaaggggata cagcgtgggc
     1501 tggagcgcga aactttgcgt gttaatgctg atggcacact ggcaacaaca ggtcatcctg
     1561 aagcattagg ttctgcactg acccataaat ggattactac cgattttgcg gaagcattgc
     1621 tggaatttat tacaccagtg gatggtgata ttgaacatat gctgaccttt atgcgcgatc
     1681 tgcatcgcta tacggcgcgc aatatgggcg atgagcggat gtggccgtta agtatgccat
     1741 gctacatcgc agaaggtcag gacatcgaac tggcacagta cggcacttct aacaccggtc
     1801 gctttaaaac gctgtaccgt gaagggctga aaaatcgcta cggcgcgctg atgcaaacca
     1861 tttccggcgt gcactataat ttctctttgc caatggcatt ctggcaagcg aagtgcggtg
     1921 atatctcggg cgctgatgcc aaagagaaaa tttctgcggg ctatttccgc gttatccgca
     1981 attactatcg tttcggttgg gtcattcctt atctgtttgg tgcgtctcca gcgatttgtt
     2041 cttctttctt gcaaggaaaa ccaacgtcgc tgccgtttga gaaaaccgag tgcggtatgt
     2101 attacctgcc gtatgcgacc tctcttcgtt tgagcgatct cggctatacc aataaatcgc
     2161 aaagcaatct tggtatcacc ttcaatgatc tctacgagta cgtggcgggc cttaaacagg
     2221 caatcaaaac gccatcggaa gagtacgcga agattggtat tgagaaagac ggtaagcggc
     2281 tgcaaatcaa cagcaatgtg ctgcagattg aaaacgaact gtacgcgccg attcgtccaa
     2341 aacgcgttac ccgcagtggt gagtcgcctt ctgatgcgct gttacgtggc ggcattgaat
     2401 atattgaagt ccgctcgctg gatatcaacc cgttctcgcc gattggtgta gatgaacaac
     2461 aggtgcgatt cctcgacctg tttatggtct ggtgtgcgct ggctgacgca ccggaaatga
     2521 gcagtagcga acttgcctgt acacgcgtta actggaaccg ggtgatcctc gaaggtcgca
     2581 aaccgggcct gacgctgggt atcggctgcg aaactgcgca gttcccgtta ccgcaggtgg
     2641 gtaaagatct gttccgcgat ctgaaacgcg tcgcgcaaac gctggatagc attaacggcg
     2701 gcgaagcgta tcagaaagtg tgtgatgaac tggtcgcctg cttcgataat cccgatctga
     2761 ctttctctgc ccgtatctta agatctatga ttgatactgg tattggcgga acaggcaaag
     2821 cgtttgcaga agcctaccgt aatctgctgc gtgaagagcc gttggaaatt ctgcgcgaag
     2881 aggattttat agccgagcgc gaggcgtctg aacgccgtca gcaggaaatg gaagccgctg
     2941 ataccgaacc gtttgcggtg tggctggaaa agcacgcctg acagaaaaga aaaaggccac
     3001 tcgtgagtgg ccaaaatttc atctctgaat tcagggatga tgataacaaa tgcgcgtctt
     3061 tcatatactc agactcgcct gggaagaaag agttcagaaa attttaaaaa aaattaccgg
     3121 aggtggctaa atgccgttgt tagatagctt cacagtcgat catacccgga tggaagcgcc
     3181 tgcagttcgg gtggcgaaaa caatgaacac cccgcatggc gacgcaatca ccgtgttcga
     3241 tctgcgcttc tgcgtgccga acaaagaagt gatgccagaa agagggatcc ataccctgga
     3301 gcacctgttt gctggtttta tgcgtaacca tcttaacggt aatggtgtag agattatcga
     3361 tatctcgcca atgggctgcc gcaccggttt ttatatgagt ctgattggta cgccagatga
     3421 gcagcgtgtt gctgatgcct ggaaagcggc aatggaagac gtgctgaaag tgcaggatca
     3481 gaatcagatc ccggaactga acgtctacca gtgtggcact taccagatgc actcgttgca
     3541 ggaagcgcag gatattgcgc gtagcattct ggaacgtgac gtgcgcatca acagcaacga
     3601 agaactggcg ctgccgaaag agaagttgca ggaactgcac atttagtcag tttaagcaga
     3661 agaggccggg ctggtgaaaa caccgcccgg cttttgttac ctgtcagtca tccgatattc
     3721 cacgctatgt gcttcatcag gttgaggaac ttccagcctt gaggccatcc gcatcacgac
     3781 cgcttcagga actgcatact cgcgtctggc attttgctgc ttccattgcg cccatggcac
     3841 ttccagataa acgattttta ctcgtgcgcc gtaagccgta aacagactga tgagctggct
     3901 gcgcagctgg cgggtaatgt tcgttgcatt ccagatgaac ggctttttct ggcgtaaaaa
     3961 aacgcgcgcc tcttcttttg cctgttgcac aatacgcccg gtggcggttt tgtcgtccgg
     4021 actggcattg attcgccgcc gcatatcatc aaggctgata acgtccattc cctggcactg
     4081 ttcactgata tagcgatctt tgcccatacc cggtaagcca cacagcaaaa taacctcaaa
     4141 aggttcagcc tcccagggaa caaaatcagg agaactttgc tcatgagtca ggtagtgcca
     4201 gcgtgcggaa tcagaaacga acgggcgcat ttttccccag cattgttgct catggcaaaa
     4261 cagctcgaac agatcgatgc gttccagcat tgattgctga tccgggcttt ggcgaccaag
     4321 cagatcggca cgagcaagca aagcgagcag acgcgtgtcg atgcgcattg cagccgtgag
     4381 cagcaaacgc tccggttccg gacgttccag taaccatagc ggtaacccat gcagacgcac
     4441 cagcgcaaca atttgctcgc gtagcacgaa cggcgttggg atatcgcgcc ataaaatttg
     4501 tcgggccgtc agttcgcccc gacgagcatg gccaggcgac tgaatacgtc cgttttcctg
     4561 cacggtagtg ctgcgctttt caacatcatg caataacgcc gccgcccata aaacttcctg
     4621 ctgttgagcg gggagttgct ggaattctgg ctgagtaacg agggcgttca gcaccatttc
     4681 ggtatgcacg ccgacgtcac cttcaccatg atgctccggg tcttgtggcg tgtgatgcat
     4741 ctcctcaacc cagctaaaac gctgacgtag cgccgaccag cgtttgtcgt cggtaagctg
     4801 ccaaatcatg actgttctcc ataatcgaac tcatgggcca tccgcgcccg ttgccagtgt
     4861 cgtttccagt ggacggtggt tttgacatga tttttgcgca catacttgaa gacgttatgc
     4921 gaaaagtcag cgacggaaaa cgcatcacga ttcctgctga caatgccttc cagcgtacag
     4981 ggttgcccgg tttgcgtatc ccagggatcg aacgcgccgc gcgcattggt cagcgccaga
     5041 aaatcacgtt gccagctttt ttcatcattc cccggttgcg ggccggagat ttcgggaacg
     5101 caggggaaat caaacagagc ggcgtaaaac tgcacctctt cccagctcag ccacatatcc
     5161 tggcagcgaa cggcaaacag ataaaaatcc tgctccaggg cgcgatattc aatcgaatgc
     5221 acggcataaa gattttcacc aaacaactcc agatcgccga gatcgttttt cagcagttgc
     5281 cagcgttggc gaattttata ggtccatgcc gactgcgtag gcgcggcatg ggaacgggca
     5341 aacacgccgt agcggttaag gcagttattt tcaccgtcga gtttttctgt gtgcaccaat
     5401 tgcgtgatgg cctgtaaatc ctgccagtaa tcggcattaa tgcggtcatc gctggtggtg
     5461 ccgggggaga aaggataatg ccaggttctc ccgtattttc gttgggtatt cataataatc
     5521 cattattttg tgtgtaataa atgtacgccc gcgagggtgc gagttatggg gttaaaagaa
     5581 aaatgtgagt atcacccgcg aaagcgaggg cgaaagaaaa agagaattaa caacctgagc
     5641 gaatgctcag aaccggttaa tggtgaatgg aagagtttgg ggcggcgaaa agatacaccg
     5701 aatgagtgga taaaacaacc gtttgcaaaa tgagcaaata aaaagggagc cgcagctccc
     5761 tttgacaacc ttagtgcgca ccgcctccgc cgccacctgc accaaatggc ggtttagcaa
     5821 accacaccag ccccagcagg acgaggaata ttccggctga catccagaag atctcattgg
     5881 cggaaataat cagcccctga ttagtgatct gctgggcaat ccagcctgat gcctgttgtt
     5941 gcgtcatccc cagcccttcc agttggctgt acatcgcctg agcgttcggg ttaaacgggt
     6001 ttaccgactc ggttaactgt gcgtggtgca tcgactcgcg gttagtccac atggtggtgg
     6061 tgatggatgt gccgatagac cccgccagcg ttcgcgtaaa gttggagagg ctcgatgccg
     6121 ccgccagtcg ttccggtggc aaaccggaca gcgtaatggt ggtcagcggc ataaagaagc
     6181 aggccaccgc aaacccctgg ataaactgcg gccaggccga cgcgccaaaa tccatacctg
     6241 gttcaaaggt ataggcgcgc cagtagaagc agacggcata cataataaag ctgaaggtta
     6301 ccagccgccg catatccagt ttatgcgcga agcggccgat aatcggcgac aggatcaccg
     6361 gaataatccc taccggcgca gaggccagac ctgcccacgt tgccgtgtaa ccgtagacct
     6421 cctgcaacaa ctgcggcagc agaacaatcg cgccgaagta gagcatatac gcgaggctga
     6481 tacacaagca gccgatggtg aagttgcgcg acttaaacaa cgacagatcg actatcgggt
     6541 tatcgtcggt cagctcccag acaatcagga agcagatagc caccactgcc accacggtaa
     6601 ggatgataat ttcctgtgat gagaaccagt ccagctcttt accgcggtcg agcataatct
     6661 gcaggctacc gataccaata accagcagtg ccagccccac ggcatcaatc cgccgccgtt
     6721 cggtgcgggt ttcacgtccg cgcagggttt gcagtgtcat caacaccacc gccacgccaa
     6781 tcggtacgtt gatgaagaat atccagcccc agtggtaatt gtcactgata tacccgccga
     6841 ggatcgggcc gcaaattggc gcgacaatca ccgtcatcga ccacaacgcc agcgcgatcg
     6901 agcgtttggc tggcgggtag ttattaagca atagactttg cgaaagcggg atcagcggcc
     6961 cggcgacaat cccctgaatc acgcggaaga agatcagcat attcaggctg ctggagacac
     7021 cacacgccca cgacgcaata gcaaaggcga tggtggacca gaggaacagt ttcacttccc
     7081 cgacgcgctt tgccagccag ccggtaagcg ggatcgagat ggcattcgcc accccgaaag
     7141 aagtgattac ccacgttccc tggctgagcg atgagcccag attcccggcg atagtgggga
     7201 tcgccacgtt agcaatggtg gagtccagca cctgcatgaa tgtcgccagt gacagcgcaa
     7261 tcgtcataat gaccagttgc gcgccttcca gcggtttttg ctgttgcatc acacgcacct
     7321 ctggattagc cagcgttagc ttttacgata tcgtcgatca gtttattgac aggtgccagg
     7381 ctgatttcac gcgcggtgct taccgctacc ggtgtggaac gtactttatt tgccagtacc
     7441 tgaccgtcac ggttagtggt attgacgctc accagcgtgg acaaaccgat acgcagcggg
     7501 tattgctcga gctgtttctg gtccagttca atacgaacag gcagacgctg aacgactttg
     7561 atccagttac cggttgcatt ttgcgccgga agcagtgaga acgcgctacc tgtgcccata
     7621 tccagaccaa ccacttttcc ggtgtatttc acatcatcgc cgtaaatatc ggtggtgatg
     7681 gtgaccggct gaccgatacg catattggca atctgcgtct ctttaaagtt ggcatccacc
     7741 cacatattgg tggctggaac gaccgccatc agcggcgtcg ttgggctaat ttgcgcccca
     7801 ggctgtaccg cgcggcggga gacataaccg gtcatcggac tgacaatacg ggtacgctcc
     7861 agcgccagcc aggcgttacg tacttcggtg gcagcctgtt gcacggcagg ctgatcttcc
     7921 agtttagtcc ccagaatcat cgcctgattg gcattgtatt gttgaatagc gacgtccagt
     7981 tgtgcctggg cactggtaac ggcgtcgcgg gcgtgttgca gctcttcgcg accaatcagg
     8041 ttggcattgc ccagcggcac acggcggttg tagtcgcttt gtgctttcgc gagggcgatt
     8101 ttttgcacct caatattcgc ctgcaactgt ttgctgttaa tcatcagctg gtgggtttgg
     8161 cgaacgctgg aagccagtgc agttttggct ttttcaaacg cctggcgagc atctgtcggg
     8221 tcgagagtga ccagcacatc gccttctttt acaaaatcgg tgttatcggc ccagactttc
     8281 gtcacgctgc cagacacctg agacataatt tgaatctgat tccctgccac gtatgcgtca
     8341 tcagtttctt cgaaatgacg cagtaccaaa aaccaataaa tccctatcgc tacggcaata
     8401 attataaaga gcaaggtgag aaggaggagc agacgcttac gtttgccgct cttctttacc
     8461 ggttgctgcg gggtttgagt ctccgcattt gcgctcatat tgttctccac gatcttctta
     8521 actcatcggc tgagccgacc tgcttgccaa aaaggccagc atgttgcgat gctggccagt
     8581 cattttttct tttataaatc tggatttttg agcgagatga cgcgttagct catcgcttcg
     8641 agaaccacac cgtcttgttc catctggtcg agacgggaga gcaatttgcg ggtgatttgc
     8701 tcgagctgat ctttttctgt tgtgctgagc gcggaccaga gttgatgcag gcagttatgc
     8761 tgcggtggta aaacctcgcg caaaaactcg tgaccttttt ccgttaattg cagatgcagg
     8821 cagcggcgat cgttatcgct ttcacgacgt tcgatccaac cgcgtttttc cagttcatcg
     8881 gcaatacgcg tcgcgttggt acgggatgat ccaagagcac aactcaattc agaaggctga
     8941 atactgtggt tttcctgaga ctccagcgta atcaacgcca taaacaacgt ctcgttaatc
     9001 ccctgagcct tcagcatttt attgcggttc tccagcagct tgctttgcat gtgcatgcaa
     9061 agacgggtca gaaggatctc ctgataagga aaatcttcgt ggcggctggc gcgaaatttt
     9121 agcatttgtt caatgggcgt aaacgaacta tccatttggg tatgacctca ttaatttcag
     9181 ccgatatagt aacgacagtg acaaataaag taaatgtatt gttttagaaa aatgattctt
     9241 gtgggttata taatcgccat cactttccag gcgagcccat aggccagcgc actaagcagt
     9301 gttgggatga taatgctgcg tgttttatag aaactggcac ccagtaccgc gaagccgacc
     9361 agagtgggca cgaaacggcg tgtatcgtgc atcacttctg gtgcggtaga gacaaccagc
     9421 agagcgcata tcgaggcgat gccaatggtg tcgagcaaaa tacctaccgc gccacgtttg
     9481 gttgggcggg cattacccac acgcaggcgc agcggcaaat agcggaagca ataattcgcc
     9541 gcgccaacca gtaacccaag cagcagaacc tcatagctca tcgggcgctc cttgccagaa
     9601 tgcctggatt aacgcagtga ggcagccaca gacaatgcct gccagaatgg cgacgggaat
     9661 agaaaatagc gttacgcctg caagggcacc aactaacgct gcggtaacgc aaagagattg
     9721 tttgcgctgg aaagaggcga gcaggaaact cataaagagt gccggaagca taaaacctaa
     9781 tgctgcttca acggcgggat atccttgcag caagccgctg ccggagaatg cccctattac
     9841 cgtaccaaat acccacgatg accatgaact gaaggcaatg ccgatcatcc agttctcgct
     9901 ccagcggcga ttattgcgta ccagttttgc ggttgcggcg gcaaaaacct catccgtcag
     9961 gccaaacgcc cacagggcag ttttcgattt ttgcagacgc tgaataatac ggctacgcag
    10021 tgacgggcca tacaaaacat ggcgaacatc cattgccatg acggtcagtg cagcaaccca
    10081 caaactactc ccggctgcca gcatcgcggt aatgacgaac tggctcgcgc ctgcataaat
    10141 gatgcaggag aaaaaaacgc tttcgagagg agagaatccc agacgggtcg cattcagacc
    10201 gaacgcaaag gccaccggaa tataactaat aacaatcggt aaactgtctt tgcatccttc
    10261 catgaaggtc gccgaaccag gagcaggcag tggagtaggg ctttccatag agtgtacgct
    10321 taacgattgt taatattata aatagactga atgaatatct taaccttatc agactgatgg
    10381 gcttcttaac acccttataa gtgtaaagcc acgaaaacgg ttgctgattg cttaaattgc
    10441 ggcttcctga cgatgaaaac ctcgccacca gaccagtaag ttaaccaggg caatcgtcgc
    10501 gccagccaga caaacgccag cccaaccgcc atgttgccag actgaggctg aaattagcga
    10561 accggcggca ccgccaatga agtagctggt catgtaacct gcggtcaggc gattacgcgc
    10621 atcagggtgt attcgataaa ttaccgtctg gttagtgata tgcacgccct gcacggtgag
    10681 atccagcacc aggatgccga taatcagcgc cagtacggaa gtgtgaccaa accagatcgc
    10741 cagccatgga agtaacagca gcagcagacc gaaagttgtg gtgtggtgcg atttgccctt
    10801 atcggcaaat ccgcccgccg gacgagcgcc caacgctccg gctgctcccg caagtccaaa
    10861 cagaccaatg acgccatcgc tgtaattaaa aggcggagcg gcgagcaaaa aggccatcga
    10921 ggtccagaga atgctgaagt tggcaaaggt caggcagccc agcaacgtgc gggtgcgcag
    10981 aattttatcg ctgataaaca tgctgaaaac ggaaccaagc aactgtgggt agttgaggtg
    11041 ggtttctgat ttcatttgtg gcagaccacg ccataatgcc agcgccatca gtgccattaa
    11101 caccgaagca acccaaaaga cggtgcgcca gccgccgagg tttgccagca acccggcaac
    11161 ggttcgtgcc agcaagatcc ccaacagcag cccgctcata atggtgccga ccactttgcc
    11221 gcgtttgtcc ggtgaagcca gcgtcgctgc cagcggaacc agaatttgtg ccacgaccga
    11281 gaataaaccg gttaatgccg taccgaggat catcatcgcc agcgactgac tgctggcagt
    11341 gattaacatg ccgcatgccg ccagtaaggt catcgagaca atcaggcggc ggcgttcaaa
    11401 catatcaccg aggggaacaa gaaacagcag acctgcggca tagcccaact gcgcggcggt
    11461 aacaataaag cctgccgaac tggcggaaag ggaaaagtta cgcgcgatgg tgtcgagcaa
    11521 tggctgggcg taatagttac tggctaccgc caggccggtg gcgatagaca tcagcacgat
    11581 cagcgccggg ctaagctcat gattaggttt agtcattatt tttgttcatc aggtgatgat
    11641 taagctggaa atcaataata gcggatgttg atgaatatgg gatgaaagat agattgttga
    11701 aatgtgcggt gtggtttgta ggtatgataa gacgcaccag cgtcgcatca ggcattgtgt
    11761 acagagcggc ggatgaggca aaaacgcctt atccgcccga ataaaaatta cttctgcgct
    11821 gccagcgcct cattcaccca gccatcgaac tgctgctggt gggctttgat ccagccatca
    11881 acgtggccct gaatatcgcc ttctgaggct ttgccgtcat gcataatggc gttctgggcg
    11941 ttaatatcag ccactggtaa ctgcataatg gcaaacagtt tcgctgctgc cgggtttttc
    12001 tcggcccagg ctttgttggc aacgatatgc atggtactga ccgggaagcc gtaattcgcg
    12061 ccattcggca gtttggtatc ggcattttta tcgcccggca gtgcggagaa cggcacctgc
    12121 aaccagacca catctttccc cggtttcagt tcgttactca cccagtacgg cgtccaggtg
    12181 tagtaaaaca ccggcttacc ctctttgtaa cgactgatgg tgtcagccat catcgctgcg
    12241 tagttcccct gattatgcgt cacggtattg gtcagtccat acgcggcaag ctggtggttg
    12301 atcgcacctt cgcagcccca gcctgggtta cagccggtta aatccgcttt accgtcgccg
    12361 ttggtatcga acagtttggc gatcttcgga tctttaagtt gcgcgatgtt ggtaattttg
    12421 tactggtcgg cagttttctt atcgatcagg taaccctgtg ccgcgccgtt aacaaatact
    12481 ccttcacgat aaaatttctt atcgccaccg gcagcttcgt acatgttgtc atgcagtggc
    12541 gtccagttca ctgcggtgaa ggttgcatcg ccggaagcaa gcgaggtgta gccaacgttg
    12601 taatccactt cgctgggttt attgacggta taacctaatt tctccagcgc gcggctgacc
    12661 agcagcgtct ggaaagtttc ttcggtgatg gtgctctgaa ccggattaac agtaatgcct
    12721 ttgcccggca ggtcggcagc aaaagtttgt gtagagataa gcgtggcaaa cgctgtcgca
    12781 aaaagtacgc tatgtcgcat tgttattcct ttttttggca gggtgataat gcccggcgtc
    12841 accgggcaag tgcagagtta cttaatgaat gggcgggtca gcagaccgac agggccagtg
    12901 gtgtaccagc gacggttgcc gcgactgcgt gagtcgcgcc caacggcctg cgtcaggcgg
    12961 tcgaggataa tggcgaggat cacaatcccg acgccgccaa cggtggcaag ccccatatcc
    13021 agacgaccga taccgcgaag caccatctga cccagcccgc cgacggcaat catcgaggcg
    13081 atgaccacca tagaaagggc cagcatcagc gtctggttaa cgcccgccat aatggtcggc
    13141 atcgccagcg gtaactgaac tttgaacagc atctggcgcg ggctggcacc gaatgagcgc
    13201 gaggcttcaa tcagatccgc cggaacctgg ttaattccca gaatcgtcag acggataatc
    13261 ggcggcagcg caaagatgat cgtcaccacc acgcccggca cgttaccgat accaaacagc
    13321 atgacgattg gtaccagata aacaaacgcg ggcgtggtct gcatggcatc aagcagtgga
    13381 cgaataattt tcgccgctcg cggacttctc gccagccaga tccccaacgg caaaccgatg
    13441 acgatacaga acagcagggc ggttagcacc agcgccagag taaccattgc ctgtgaccag
    13501 gcaccgattg cgccgatggc aatcagcgaa accagtgtcg ccacgcccat tccgacaccg
    13561 gaaatttgcc aggcgatgag agcgaaaacg ataatcgcca ccggagcggg catacccagc
    13621 agcaattgct ggaaaccgtt gaggatgtaa tcaaccggaa cgcgcacgcc ctgaaagacg
    13681 ggacgaaaat gggtaacgac ccagtcgatc ccttcagtga cccaactgtc gagcgggatc
    13741 agcgttttat ggaacggatc gagaatatta aaatgctcga cgtttggcgc aggcgtactg
    13801 gtcagccagt cagcaccacc gccgtcagtc ggtgcagcag ccggtgtacc ccaggcgtct
    13861 gcggactgtg cggcactgtc cgccgctggc gtggtatccc acggattatt ttgatcagcc
    13921 attatttacc ccctcacgat ctaaagcgcg cagcagcatt cctttcgaaa tgatgccgac
    13981 atactgttgg tcctcatcga ccacgggcac cgcacagggg gcctgtccga catgagagag
    14041 caactcgcta agaggcgttt gtgcatcgac cgctaacggc gcatcaatca gcgccgcatc
    14101 aagaccttgc tgctgcgtta acgcggcttt aagcgaatcg atagacacag cgccgacaaa
    14161 cttattaccg cgttcgatga cgtagccata ttcgcgatct tcatcctgca ataatttcag
    14221 tgccgaacgc gggccgaagc cgggggtttt acgaattaag ccgttcggtg tacgacgggc
    14281 aatatctttc gcactgaaca cctggctaat atcgacgcca cggaagaagg tacggacata
    14341 atcattcgcc ggattattga gaatttcatc cggtgtgccg acctgtacta cttcaccatt
    14401 ttgcataatg gcaattcggt cgccaatacg catggcttca tcgagatcgt gggaaataaa
    14461 gacaatggtg cgctgatgtt tcgcctgtaa ttttaccagc tcatcctgca tctcggtgcg
    14521 aattaatgga tcgagcgccg agaaggcttc gtccattaat aatatatccg gattaatcgc
    14581 taacgcgcgg gctaatccca cacgttgacg catcccgcca gagagttcat ccgggtagct
    14641 gtgggcataa ttttccagcc cgacctgacg cagtgcatca agggcttttt cacggcgttc
    14701 ttcggcatta attccggcca gttccatacc gaacgcagta ttgtccagca cggtcatatg
    14761 cggcattaag gcaaaggact ggaagaccat cgcaatcttt tttctgcgca cctcacggag
    14821 ttcggcgtcg gatattttgg caatatccac accatcaatc agcacttgcc cgcgggtggg
    14881 ttcaatcagg cgattgagaa ggcgtaccat tgtggattta cccgaaccgg ataatcccat
    14941 gatgacaaat atctcgcctt cttcaatggc cagactggcg tctttaacgc caagcgatag
    15001 cccagttttt tccagaattt gttcttttga aagtccttgt tcgatatatt tgaaagctc
//
LOCUS       DAZFLD010000122        15012 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.123, whole genome shotgun sequence.
ACCESSION   DAZFLD010000122 DAZFLD010000000
VERSION     DAZFLD010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15012
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.123"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(50..2329)
                     /gene="maeB"
                     /locus_tag="ACHKBL_003145"
     CDS             complement(50..2329)
                     /gene="maeB"
                     /locus_tag="ACHKBL_003145"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxaloacetate-decarboxylating
                     malate dehydrogenase"
                     /protein_id="HFO5819980.1"
                     /translation="MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVA
                     APCLEIEKDPLKAYKYTARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFA
                     GIDVFDIEVDELDPDKFIEVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
                     DDQHGTAIISTAAILNGLRVVEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVV
                     CDSKGVIYRGREPNMAETKAAYAVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKK
                     MARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDV
                     GATAINEEMKLAAVRAIAELAHAEQSEVVASAYGDQDLSFGPEYIIPKPFDPRLIVKI
                     APAVAKAAMESGVATRPIADFDVYIDKLTEFVYKTNLFMKPIFSQARKAPKRVVLPEG
                     EEARVLHATQELVTLGLAKPILIGRPNVIEMRIQKLGLQIKAGVDFEIVNNESDPRFK
                     EYWTEYFQIMKRRGVTQEQAQRALISNPTVIGAIMVQRGEADAMICGTVGDYHEHFSV
                     VKNVFGYRDGVHTAGAMNALLLPSGNTFIADTYVNDEPDAEELAEITLMAAETVRRFG
                     IEPRVALLSHSNFGSSDCPSSSKMRQALELVRERAPELMIDGEMHGDAALVEAIRNDR
                     MPDSPLKGSANILVMPNMEAARISYNLLRVSSSEGVTVGPVLMGVAKPVHVLTPIASV
                     RRIVNMVALAVVEAQTQPL"
     gene            2618..3568
                     /gene="tal"
                     /locus_tag="ACHKBL_003146"
     CDS             2618..3568
                     /gene="tal"
                     /locus_tag="ACHKBL_003146"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HFO5819981.1"
                     /translation="MNELDGIKQFTTVVADSGDIESIRHYHPQDATTNPSLLLKAAGL
                     SQYEHLIDDAIAWGKKNGKTQEQQVVAACDKLAVNFGAEILKIVPGRVSTEVDARLSF
                     DKEKSIEKARHLVDLYQQQGVEKSRILIKLASTWEGIRAAEELEKEGINCNLTLLFSF
                     AQARACAEAGVFLISPFVGRIYDWYQARKPMDPYVVEEDPGVKSVRNIYDYYKQHHYE
                     TIVMGASFRRTEQILALTGCDRLTIAPNLLKELQEKVSPVVRKLIPPSQTFPRPAPMS
                     EAEFRWEHNQDAMAVEKLSEGIRLFAVDQRKLEDLLAAKL"
     gene            3588..5591
                     /gene="tkt"
                     /locus_tag="ACHKBL_003147"
     CDS             3588..5591
                     /gene="tkt"
                     /locus_tag="ACHKBL_003147"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFO5819982.1"
                     /translation="MSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFL
                     KHNPTDPTWYDRDRFILSNGHASMLLYSLLHLTGYDLPLEELKNFRQLHSKTPGHPEI
                     GYTPGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGCLMEG
                     ISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVIHEIDGH
                     DPQAVKEAILEAQSVKDKPSLIICRTVIGFGSPNKAGKEEAHGAPLGEEEVALARQKL
                     GWHHPPFEIPKEIYHAWDAREKGEKAQQSWNEKYAAYKKAHPQLAEEFTRRMSGGLPK
                     DWEKTTQKYINELQANPAKIATRKASQNTLNAYGPMLPELLGGSADLAPSNLTIWKGS
                     VSLKEDPAGNYIHYGVREFGMTAIANGIAHHGGFVPYTATFLMFVEYARNAARMAALM
                     KARQIMVYTHDSIGLGEDGPTHQAVEQLASLRLTPNFSTWRPCDQVEAAVGWKLAVER
                     HNGPTALILSRQNLAQVERTPDQVKEIARGGYVLKDSGGKPDIILIATGSEMEITLQA
                     AEKLAGEGRNVRVVSLPSTDIFDAQDEEYRESVLPSNVAARVAVEAGIADYWYKYVGL
                     KGAIVGMTGYGESAPADKLFPFFGFTAENIVAKAHKVLGVKGA"
     gene            complement(5694..6737)
                     /gene="ypfG"
                     /locus_tag="ACHKBL_003148"
     CDS             complement(5694..6737)
                     /gene="ypfG"
                     /locus_tag="ACHKBL_003148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HFO5819983.1"
                     /translation="MRYRIFLLFFFALLPTSLVWAAPAQRAFSDWQVTCNNQNFCVAR
                     NTGDHNGLVMTLSRSAGAHTDAVLRIERGGLKSPDASEGEIAPRLLLDGEPLALSGDK
                     WRISPWLLVTDDTATITAFLQMIQEGRAITLRDGNQTISLSGLKAALLFIDAQQKRVG
                     SETAWIKKGDEPPLSVPPAPALKEVAVVNPTPTPLSLEERNDLLDYGNWRMNGLRCSL
                     DPLRREVNVTALTDDKALMMISCEAGAYNTIDLAWIVSRKKPLASRPVRLRLPFNSGQ
                     ETNELELMNATFDEKSRELVTLAKGRGLSDCGIQARWRFDGQRFRLVRYAAEPTCDNW
                     HGPDAWPTLWITR"
     gene            complement(6863..7438)
                     /gene="nudK"
                     /locus_tag="ACHKBL_003149"
     CDS             complement(6863..7438)
                     /gene="nudK"
                     /locus_tag="ACHKBL_003149"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
                     /protein_id="HFO5819984.1"
                     /translation="MTQQITLIKDKILSDNYFTLHNITYDLTRKDGEVIRHKREVYDR
                     GNGATILLYNAKKKTVVLIRQFRVATWVNGNESGQLIETCAGLLDNDEPEVCIRKEAI
                     EETGYEVGEVRKLFELYMSPGGVTELIHFFIAEYSDSQRANAGGGVEDEDIEVLELPF
                     NKALEMIKTGEIRDGKTVLLLNYLQMSHLMD"
     gene            complement(7506..9485)
                     /gene="aegA"
                     /locus_tag="ACHKBL_003150"
     CDS             complement(7506..9485)
                     /gene="aegA"
                     /locus_tag="ACHKBL_003150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     AegA"
                     /protein_id="HFO5819985.1"
                     /translation="MNRFIMANSQQCLGCHACEIACVMAHNDEQHVLSQHHFHPRITV
                     IKHQQQRSAVTCHHCEDAPCARSCPNGAISHVDDSIQVNQQKCIGCKSCVVACPFGTM
                     QIVLTPVAAGKVKATAHKCDLCAGRENGPACVENCPADALQLVTDAALSGMAKSRRLR
                     TARQEHQPWHASTAAQEMPVMSKVEQMQATPARGEPDKLAIEARKTGFDEIYLPFRAD
                     QVQREASRCLKCGEHSVCEWTCPLHNHIPQWIELVKAGNIDAAVELSHQTNTLPEITG
                     RVCPQDRLCEGACTIRDEHGAVTIGNIERYISDQALAKGWRPDLSHVTKVDKRVAIIG
                     AGPAGLACADVLTRNGVEVTVYDRHPEIGGLLTFGIPSFKLDKSLLARRREIFSAMGI
                     HFELNCEVGKDVSLNSLLEQYDAVFVGVGTYRSMKAGLPNEDAPGVYDALPFLIANTK
                     QVMGLEELPEEPFINTAGLNVVVLGGGDTAMDCVRTALRHGASNVTCAYRRDEANMPG
                     SKKEVKNAREEGANFEFNVQPVALELNEQGHVCGIRFLRTRLGEPDAQGRRRPVPVEG
                     SEFVMPADAVIMAFGFNPHGMPWLESHGVTVDKWGRIIADVESQYRYQTTNPKIFAGG
                     DAVRGADLVVTAMAEGRHAAQGIIDWLGVKSVKSH"
     gene            9691..11391
                     /gene="narQ"
                     /locus_tag="ACHKBL_003151"
     CDS             9691..11391
                     /gene="narQ"
                     /locus_tag="ACHKBL_003151"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
                     /protein_id="HFO5819986.1"
                     /translation="MIVKRPVSASLARAFFYIVLLSILSTGIALLTLASSLRDAEAIN
                     IAGSLRMQSYRLGYDLQSGSPQLNAHRQLFQQALHSPVLTNLNVWYVPEAVKTRYAHL
                     NANWLEMNNRLSKGDLPWYQANINNYVNQIDLFVLALQHYAERKMLLVVAISLAGGIG
                     IFTLVFFTLRRIRHQVVAPLNQLVTASQRIEHGQFDSPPLDTSLPNELGLLAKTFNQM
                     SSELHKLYRSLEASVEEKTRDLHEAKRRLEVLYQCSQALNTSQIDVHCFRHILQIVRD
                     NEAAEYLELNVGDNWRISEGQPNPELPMQILPVTMQETVYGELHWQNSHVSSSEPLLN
                     SVSSMLGRGLYFNQAQKHFQQLLLMEERATIARELHDSLAQVLSYLRIQLTLLKRSIP
                     EDNATAQSIMADFSQALNDAYRQLRELLTTFRLTLQQADLPSALREMLDTLQNQTSAK
                     LTLDCRLPTLALDAQMQVHLLQIIREAVLNAMKHANASEIAVSCVTAPDGNHTVYIRD
                     NGIGIGEPKEPEGHYGLNIMRERAERLGGTLTFSQPSGGGTLVSISFRSAEGEESQLM
                     "
     gene            11555..14668
                     /gene="acrD"
                     /locus_tag="ACHKBL_003152"
     CDS             11555..14668
                     /gene="acrD"
                     /locus_tag="ACHKBL_003152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease AcrD"
                     /protein_id="HFO5819987.1"
                     /translation="MANFFIDRPIFAWVLAILLCLTGTLAIFSLPVEQYPDLAPPNVR
                     VTANYPGASAQTLENTVTQVIEQNMTGLDNLMYMSSQSSGTGQASVTLSFKAGTDPDE
                     AVQQVQNQLQSAMRKLPQAVQNQGVTVRKTGDTNILTIAFVSTDGSMDKQDIADYVAS
                     NIQDPLSRVNGVGDIDAYGSQYSMRIWLDPAKLNSFQMTAKDVTDAIESQNAQIAVGQ
                     LGGTPSVDKQALNATINAQSLLQTPEQFRDITLRVNQDGSEVRLGDVATVEMGAEKYD
                     YLSRFNGKPASGLGVKLASGANEMATAQLVLNRLDELAQYFPHGLEYKVAYETTSFVK
                     ASIEDVVKTLLEAIALVFLVMYLFLQNFRATLIPTIAVPVVLMGTFSVLYAFGYSVNT
                     LTMFAMVLAIGLLVDDAIVVVENVERIMSEEGLTPREATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGTTGAIYRQFSITIVAAMVLSVLVAMILTPALCATLLKPLKKGEHHGQ
                     KGFFAWFNQMFNRNAERYEKGVAKILHRSLRWIVIYVLLLGGMVFLFLRLPTSFLPLE
                     DRGMFTTSVQLPSGSTQQQTLKVVEQIEKYYFTHEKDNIMSVFATVGSGPGGNGQNVA
                     RMFIRLKDWSERDSKTGTSFAIIERATKAFNKIKEARVIASSPPAISGLGSSAGFDME
                     LQDHAGAGHDALMAARNQLLALAAENPELTRVRHNGLDDSPQLQIDIDQRKAQALGVA
                     IDDINDTLQTAWGSSYVNDFMDRGRVKKVYVQAAAPYRMLPDDINLWYVRNKDGGMVP
                     FSAFATSRWETGSPRLERYNGYSAVEIVGEAAPGVSTGTAMDIMESLVKQLPNGFGLE
                     WTAMSYQERLSGAQAPALYAISLLVVFLCLAALYESWSVPFSVMLVVPLGVIGALLAT
                     WMRGLENDVYFQVGLLTVIGLSAKNAILIVEFANEMNQKGHDLFEATLHACRQRLRPI
                     LMTSLAFIFGVLPMATSTGAGSGGQHAVGTGVMGGMISATILAIYFVPLFFVLVRRRF
                     PLKPRPE"
     gene            complement(14767..14826)
                     /gene="ypfM"
                     /locus_tag="ACHKBL_003153"
     CDS             complement(14767..14826)
                     /gene="ypfM"
                     /locus_tag="ACHKBL_003153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpfM"
                     /protein_id="HFO5819988.1"
                     /translation="MIERELGNWKDFIEVMLRK"
BASE COUNT         3519 a         3992 c         3914 g         3587 t
ORIGIN      
        1 aaactgctta ccctgaatat tcagggtaag cgtgagagtt aaaaaaaaat tacagcggtt
       61 gggtttgcgc ttcaaccacg gccagcgcca ccatattgac gatacgacgt accgatgcga
      121 tcggcgttaa cacgtgaacc ggtttcgcca cacccatcag caccggaccg acagtcacgc
      181 cttccgagct ggaaacgcgc agtaagttgt aactaatgcg ggcagcttcc atgttcggca
      241 tcaccagaat attagcggaa cctttcaaag ggctgtccgg catacggtca ttccgaatcg
      301 cttccaccag cgctgcatcg ccgtgcattt caccatcaat catcagttct ggtgcacgtt
      361 ccctgaccag ttccagcgcc tgacgcattt tgctcgacga cgggcagtca gaagaaccaa
      421 agttggagtg cgacaacaga gccacacgcg gctcaatacc aaaacgacga acagtttctg
      481 ccgccatcaa ggtgatctcc gccagctctt ctgcatcagg ctcatcattg acgtaggtat
      541 cggcaataaa ggtgttacca ctcggcaaca gcagcgcgtt cattgcacct gcggtgtgaa
      601 cgccatcgcg ataaccaaag acatttttca ccacgctaaa gtgttcatga taatcgccca
      661 ccgtaccgca aatcattgca tcggcttcgc cacgctgaac catgatcgcg ccgatcactg
      721 tcgggttact gatcagcgca cgctgcgcct gctcctgagt gacaccgcga cgcttcatga
      781 tctggaagta ttcggtccag tactctttaa agcgcggatc ggattcgtta ttgacgatct
      841 caaaatcaac gcccgccttg atctgcaagc ccagtttctg aatgcgcatt tcgatcacgt
      901 tcggacgacc aataaggatc ggtttcgcca gtcccagcgt taccagttcc tgagtggcat
      961 gcagaacgcg cgcctcttcc ccttccggca gaactacgcg cttcggcgct ttgcgagcct
     1021 gggagaaaat cggcttcata aacaggttag ttttgtaaac gaactccgtc agcttgtcga
     1081 tgtagacgtc gaaatcagca atcggacgcg tcgccacgcc cgactccatc gccgctttcg
     1141 ccaccgcagg agcgatctta acgatcaagc gcggatcaaa cggttttgga atgatgtatt
     1201 ccggaccaaa gctcagatcc tgatcgccat acgctgaagc caccacttca ctctgttccg
     1261 catgggccag ttctgcaatc gcgcgtaccg ccgccagttt catctcttcg ttgatggcgg
     1321 ttgcgccaac gtccagcgcg ccacggaaga taaacgggaa gcaaaggacg ttgttcacct
     1381 ggttcgggta gtcggaacgg ccggtacaga tgatggcatc cggacgcact tctttcgcca
     1441 gcggcggcag aatttccggt tccgggttcg ccagcgccag gatcattggc gcacgggcca
     1501 ttttcttcac catttcctgg gtcagcactt tcgggccgga acagccgagg aaaatatccg
     1561 cgccttcaat cacatcatcg agggtacgtt taccgtcatc caccaccgcg tatgcggctt
     1621 tggtttccgc catgtttggc tcacggcccc gatagataac gccttttgaa tcgcaaacca
     1681 cgatgttatg tttttgcaga cccagcgcta ccagcaggtt catacaggcg attgctgcgg
     1741 cacccgcgcc agaaaccacc atccgcacgt cggagatgtt tttctccacc acgcgcaaac
     1801 cgttgaggat ggcggcagtg ctgataattg ccgtgccgtg ctgatcgtcg tggaataccg
     1861 gaatattcat ccgctcgcgc agtttctgtt caatatagaa acattctggc gctttaatgt
     1921 cttcgaggtt gatgccgcca aaggtcggtt cgagcgcagc gacgacttca ataaatttgt
     1981 ccgggtcgag ttcatcaact tctatgtcaa atacatcaat cccggcgaat ttcttaaaca
     2041 gaacgccctt gccttccatc accggtttgc ctgccagcgc gccaatgttg cctaacccca
     2101 gcaccgccgt accgttagag atcaccgcta ccaggttacc gcgcgcggta tatttgtagg
     2161 cttttaacgg gtctttttcg atttcaagac aaggtgcggc aacgcctggt gagtaggcca
     2221 gcgccagatc gcgctgcgtt gccagaggct tggttggaga aacctggatt ttccctggaa
     2281 ctggaaattc atggaaatca agtgcacttt gttttaactg gtcatccatt tggttgttcc
     2341 tttcacgtaa cgttcacaaa taaagtgtgt gggcaacagc ccctgcccac aacgtggcgc
     2401 acattattac cttgccggag tctacagact ttgagcaagt ccaaactctc accattaata
     2461 taatgttttg gtaataatcc tataacactg atgttacctg cttaatccag caataccatg
     2521 cctgtctgct atgctttttt gatgcgttta gcgaaatttc tcagaagtgt gaattaacgc
     2581 actcatctaa cactttactt ttcaaggagt atttcctatg aacgagttag acggcatcaa
     2641 acagttcacc actgtcgtgg cagacagcgg cgatattgag tccattcgcc attatcatcc
     2701 ccaggatgcc accaccaatc cttcgctgtt actcaaggct gccggattat cacaatatga
     2761 gcatttaata gacgatgcta tcgcctgggg taaaaaaaat ggcaagaccc aggagcaaca
     2821 ggtggtcgca gcgtgtgaca aactggcggt caatttcggt gctgaaattc tcaaaatcgt
     2881 acccggtcgc gtgtcaacag aagttgatgc acgcctctct tttgataaag aaaagagtat
     2941 tgagaaggcg cgccatctgg tggacttgta tcagcaacaa ggcgttgaga aatcacgcat
     3001 tctgatcaag ctggcttcga cctgggaagg aattcgcgcg gcagaagagc tggaaaaaga
     3061 aggtattaac tgcaacctga cgctgctgtt ttcttttgca caggcgcggg cctgtgcaga
     3121 agcaggcgtt tttctgattt cgccgtttgt cgggcgtatt tatgactggt atcaggcacg
     3181 caagccgatg gacccgtatg tggtggaaga agatccaggc gttaaatcag tacgcaatat
     3241 ctacgactac tataagcaac accactatga aaccattgtg atgggcgcga gcttccgtcg
     3301 caccgaacaa atcctcgcct taaccggctg cgaccgactg actatcgcac cgaatttgct
     3361 gaaggagcta caggaaaaag tttcgccagt ggtacgtaaa ttaatcccac cttctcagac
     3421 gttcccacgc ccagctccca tgagcgaagc ggaattccgt tgggagcaca atcaggatgc
     3481 gatggcggta gaaaaactgt ctgaaggcat tcgtctgttc gccgttgatc aacgcaaact
     3541 ggaagatctt cttgccgcca aactataaac cagccacgga gtgttatatg tcccgaaaag
     3601 accttgccaa tgcgattcgc gcactcagta tggatgcggt acaaaaagcc aattctggtc
     3661 atcccggcgc gccgatgggc atggctgata ttgccgaagt gctgtggaac gattttctta
     3721 aacataatcc taccgaccca acctggtatg atcgcgaccg ctttattctt tccaacggtc
     3781 acgcgtcgat gctgctctac agtttgctgc atctgaccgg ttacgacctg ccactggaag
     3841 aactgaagaa cttccgtcag ttgcattcga aaacccctgg tcacccggaa atcggctata
     3901 cgccaggtgt tgaaaccacc accggtcctc ttggacaagg tttggcgaac gccgtcgggc
     3961 tggcgatagc agagcgtacg ctggcggcgc agtttaatca gccggatcat gaaattgtcg
     4021 atcacttcac ctatgtgttt atgggcgacg gctgcctgat ggaaggtatc tctcacgaag
     4081 tctgttcgct ggcgggcacg ctggggctgg gcaagctgat tggtttttac gatcacaacg
     4141 gtatttccat cgacggtgaa accgaaggct ggtttaccga cgatacggca aaacgttttg
     4201 aagcctatca ctggcatgtg atccacgaga tcgacggtca cgatccgcag gcggtgaagg
     4261 aagcgatcct tgaagcgcaa agcgtgaaag ataagccgtc gctgattatc tgccgtacgg
     4321 tgattggctt tggatcgccg aataaagcag gtaaagaaga ggcgcacggc gcaccgctgg
     4381 gtgaagaaga agtggcattg gcacggcaaa aactgggctg gcaccatccg ccatttgaga
     4441 tccctaaaga gatttatcac gcctgggatg cccgcgaaaa aggcgaaaaa gcgcagcaga
     4501 gctggaatga gaagtatgca gcctacaaaa aggcccatcc gcaactggca gaagagttta
     4561 cccgtcggat gagcggtggt ttaccgaagg actgggagaa aacgactcag aaatatatca
     4621 atgaattaca ggcaaatccg gcgaaaatcg ctacccgtaa ggcttcgcaa aatacgctta
     4681 acgcttacgg gccaatgctt cctgaattgc tcggcggttc ggcggatctg gctcccagca
     4741 acctcaccat ctggaaaggt tctgtgtcgc tgaaggagga tccggcgggc aactacattc
     4801 actacggggt gcgggaattt ggcatgaccg ctatcgccaa tggcattgcg caccacggcg
     4861 gcttcgtgcc gtataccgcg acgttcctga tgtttgttga atacgctcgt aacgccgcgc
     4921 ggatggcggc actgatgaaa gcgcggcaga ttatggttta tacccacgac tcaattggct
     4981 taggggaaga tggtccgacc caccaggccg ttgagcaact ggccagtctg cgcttaacgc
     5041 caaatttcag cacctggcga ccgtgcgatc aggtggaagc ggcggttggc tggaagctgg
     5101 cggttgagcg ccacaacgga ccgacggcac tgatcctctc caggcagaat ctggcccagg
     5161 tggaacgtac gccggatcag gttaaagaga ttgctcgtgg cggttatgta ctgaaagaca
     5221 gcggcggtaa gccagatatt attttgattg ccaccggttc agagatggaa atcaccctgc
     5281 aagcggcaga gaaattagca ggagaaggtc gcaatgtacg cgtagtttcg ctgccctcga
     5341 ccgatatttt cgacgcccag gatgaggaat atcgggagtc ggtgttgcct tctaacgttg
     5401 cggctcgcgt ggcggtggaa gcaggcatcg ccgattactg gtacaaatat gttggtctga
     5461 aaggggcaat tgtcgggatg accggctatg gggaatctgc tccggcagat aagctgttcc
     5521 cgttctttgg ctttaccgcc gagaatattg tggcaaaagc gcataaggtg ctgggagtga
     5581 aaggtgcctg atggtgatcg ccggatgcta tatgttcccc tcaccctaac cctctcccca
     5641 gaggggcgag gggacggtcc gtgcgctttt taggggcgca tctgggattt tgtttaacgg
     5701 gtgatccaca acgtgggcca ggcgtctggc ccatgccagt tatcgcaggt gggttctgcg
     5761 gcgtaacgca ccaggcgaaa acgctgaccg tcaaagcgcc agcgcgcctg aatgccacaa
     5821 tcgcttaatc cgcgcccttt cgctaaggtc accagttcac gcgatttttc atcgaatgtt
     5881 gcgttcatca gttccagttc attcgtctcc tgaccgctgt tgaacggcaa acgtaaccga
     5941 accgggcgcg aggccagtgg ctttttacgc gacacaatcc atgccaaatc aatggtgtta
     6001 taggcccctg cctcacagct aatcatcatc agcgctttat catcggtcag cgcagtgaca
     6061 ttcacctctc gacgcaatgg atcaagcgag cagcgcagac cattcatccg ccagtttcca
     6121 taatccagca aatcgttgcg ttcttcgagt gagagtggcg tcggcgttgg gttaaccacc
     6181 gcgacctctt tcagcgcagg cgcgggcggt acgctgagcg gcggttcatc ccctttcttt
     6241 atccacgcgg tttcactgcc aacgcgcttt tgctgagcat ctataaacaa caacgccgct
     6301 tttaagccac tcagagaaat agtctgattg ccgtcgcgta aggtgatcgc cctcccttcc
     6361 tgaatcatct gcaaaaacgc ggtgatggtt gccgtatcat cggttactaa taaccatggt
     6421 gaaatccgcc acttgtcacc acttaacgcc aacggctcac catctaacag cagccgtggc
     6481 gctatctccc cttcagacgc atccggcgac ttcaacccgc cgcgctcaat acgtaaaacg
     6541 gcatcggtat gcgccccggc gctgcggctc agggtcatca ccagtccgtt atgatcgccc
     6601 gtattacgcg ccacgcagaa gttttggtta ttacaggtga cctgccagtc ggaaaacgcc
     6661 cgttgcgctg gtgccgccca caccaaagac gtcggcaaca aagcgaaaaa aaagagaagg
     6721 aaaatgcgat agcgcatgga cggtacgacc ccagaagttc aaacaaacag cacaagtcgt
     6781 atcttcgttg cgcaggtagc gcggctcaat cggatttatc ggattgagcc aataataaac
     6841 aacgaaatat tattgttatt tttcagtcca ttaaatgtga catttgcaaa tagttaagca
     6901 ataacaccgt cttaccgtca cgtatctcgc cagttttgat catctccagc gccttgttga
     6961 acggcagctc gagcacttca atatcttcat cttcgacacc accgccggcg ttagcgcgct
     7021 ggctgtcact gtattcggcg ataaagaagt ggattagctc agtcacaccg cctggcgaca
     7081 tatacagttc aaataatttg cgcacttcgc caacttcata acccgtctct tcaatcgctt
     7141 ctttacgaat acacacttcc ggttcgtcgt tatccagcag cccggcgcag gtttcaatca
     7201 gttgcccgct ttcattgcca ttaacccagg tagcgacgcg gaactggcga atcagaacca
     7261 cggtcttttt cttcgcgttg tacaggagga tcgttgcacc attaccgcga tcgtaaactt
     7321 cacgtttatg gcggataact tcgccatctt tgcgcgtgag atcgtaagta atgttgtgca
     7381 gggtgaaata gttatcggag agaattttgt ctttaatgag ggtgatttgt tgcgtcatac
     7441 cgactccaca gcgcgaaatg aacaattatc ttacgctgtg aagccgggtt tgtctgcgca
     7501 ggctatcagt gagatttgac tgattttacc cccagccagt caataatccc ctgtgccgca
     7561 tgacgtcctt ctgccattgc ggtaaccacc agatccgcac cacgcacggc gtcaccaccg
     7621 gcgaagattt tcggattggt ggtctggtaa cggtactggc tttccacatc cgcgatgatg
     7681 cggccccatt tgtctaccgt tacaccgtgc gactccagcc acggcatccc gtgcggattg
     7741 aagccaaacg ccataatcac cgcatcggct ggcatgacaa attcactgcc ttccaccggc
     7801 actggacgcc gacgcccctg ggcatccggc tcgccaagac gcgtgcgcag gaaacgaatc
     7861 ccgcagacgt gaccttgttc attcagttca agcgccaccg gctggacgtt aaattcgaag
     7921 ttggccccct cttcacgggc gttcttcacc tctttcttcg agcctggcat gttagcttca
     7981 tcacgacgat aagcgcaggt gacgttactc gcgccgtggc gcagcgcagt acgcacacag
     8041 tccatcgcgg tgtcgccgcc gcccagtacc acgacgttaa gtccggcagt attgataaac
     8101 ggctcttccg gtagctcttc gagtcccatc acctgtttag tattggcgat gaggaacggc
     8161 agcgcgtcat aaacgcccgg cgcatcttca ttgggtaaac ccgctttcat ggagcggtaa
     8221 gtgcctacgc caacgaagac cgcgtcgtat tgttccaaaa gcgaattcaa agagacatct
     8281 ttacccactt cacaattgag ttcgaagtga atccccatcg cgctgaagat ttcccgacgg
     8341 cgtgccagca gggatttatc cagtttgaaa gaaggaatgc cgaaagtgag caagccaccg
     8401 atttctggat ggcgatcgta caccgtcacc tctacgccat tgcgggtcaa aacatccgca
     8461 caggccagcc ctgccggacc cgcaccgata atcgccaccc gcttgtccac tttggtgaca
     8521 tggcttaagt caggacgcca acctttcgcc aacgcctgat ctgaaatgta gcgttcaatg
     8581 ttaccgatag ttactgcgcc gtgctcatcg cgaatagtac aggcaccttc acacaaacgg
     8641 tcttgcgggc aaacgcgtcc ggtaatttcc ggcagggtgt tggtctggtg agaaagctcg
     8701 acggcggcgt cgatgtttcc ggctttcacc agttcaatcc actgcggtat atggttatgc
     8761 agcgggcagg tccattcaca aacgctgtgc tcgccgcact taaggcagcg cgaggcttcc
     8821 cgttgtacct gatcggcacg gaatggcaga taaatttcat caaaaccggt tttgcgcgct
     8881 tcaatcgcca gtttatctgg ctcgccacgc gcgggcgttg cctgcatttg ctcgacttta
     8941 ctcattaccg gcatttcttg cgccgcggta ctggcatgcc acggttgatg ttcctgacgt
     9001 gcggtacgca agcggcggga tttcgccatg cctgagagtg cggcgtcagt gaccagttgc
     9061 agcgcgtccg ctgggcaatt ctcgacacaa gcaggaccgt tttcgcgccc tgcacaaagg
     9121 tcgcatttat gcgccgtggc ttttactttt cctgccgcga cgggggtcag gacgatttgc
     9181 atcgtaccaa atggacaggc caccacgcag gatttacagc caatacactt ttgctgattg
     9241 acctgaatgc tgtcatcaac gtggctgatt gcgccattag ggcagctacg ggcgcagggc
     9301 gcatcttcac aatggtgaca ggtcacggca ctacgttgct gctgatgttt gataaccgta
     9361 attcggggat gaaaatggtg ttggctcagg acatgttgct catcattgtg agccatgaca
     9421 caggcgattt cacaagcatg acaacccaga cattgctgac tgttggccat aataaaacga
     9481 ttcataacga ccttcttttt tggttgtaaa aaccttattc tttatatgag tgttgttatt
     9541 acccgactta caggggaatc ggcaatgttc atgtgcccag aataagtaac tatttcgcta
     9601 taaactgtgg cagatcaaat aatcccatca ctgactaaat tgcgtttcag cgaattggaa
     9661 cattaatgat tttttgtgga gaagacgcgt gtgattgtta aacgacccgt ctcggccagt
     9721 ctggcccggg ccttttttta cattgtgctg ctgtcgattc tttccacggg tatcgctctg
     9781 ctaacgctgg cgagcagttt acgcgacgct gaggctatca atattgccgg atcgctgcgt
     9841 atgcagagtt accgcctggg ctacgacttg caaagtggca gtccacaact caatgcacat
     9901 cgccagttat ttcagcaggc actgcattca ccggtattaa ccaatctcaa cgtctggtat
     9961 gtgccagagg cagtaaaaac ccgctatgcg catctgaatg ccaactggct ggagatgaat
    10021 aatcggctca gcaagggcga cttgccgtgg tatcaggcca atattaataa ttatgttaat
    10081 cagatagacc tgttcgtgct ggctttacag cactacgccg aacgcaaaat gctgctggtg
    10141 gtggcgatct ccctggctgg cggcatcggt attttcacgc tggtcttttt tactttgcgc
    10201 cgcatacgcc atcaggtggt tgccccgctg aatcagctgg ttaccgccag tcagcgtatt
    10261 gaacacgggc agttcgactc gccgccgctg gataccagcc tgccgaatga gcttggcctg
    10321 cttgcaaaaa cctttaacca gatgtcgagc gagctgcata aattgtaccg ttcgctggag
    10381 gcgtcagtag aagaaaagac ccgcgatctc cacgaggcca agcgtcgtct ggaggtgttg
    10441 tatcagtgtt cgcaggcgct aaacaccagc cagattgatg tgcattgttt ccgccatatt
    10501 ttgcagattg ttcgcgacaa tgaagcggct gaatatctgg agttaaatgt cggtgacaac
    10561 tggcggatta gcgaagggca gccaaacccg gaattgccga tgcagatttt accggtgaca
    10621 atgcaagaga cggtttacgg cgaactgcac tggcaaaata gtcacgtttc atcatcagaa
    10681 ccgctgctta acagtgtttc gtcgatgctg ggacgcggtt tgtactttaa tcaggcgcag
    10741 aagcattttc agcagttatt gttgatggaa gaacgtgcga ccatcgcccg cgaattgcac
    10801 gactcgctgg ctcaggtact ttcttactta cgtatccagt tgacgttact gaagcgttcg
    10861 ataccggaag ataacgccac cgcacaaagt atcatggccg atttctccca ggcgctgaat
    10921 gatgcttatc ggcagttacg cgagctgtta actactttcc gcctgacgct gcagcaggcg
    10981 gatctcccct ccgcgttgag ggaaatgctg gatacgttac aaaatcaaac cagcgccaaa
    11041 ctgaccctcg actgccgtct gccaaccctg gcgctggatg cgcaaatgca ggtgcatttg
    11101 ttgcaaatta ttcgcgaagc ggtgctgaat gcgatgaagc acgccaacgc cagcgaaatc
    11161 gccgttagct gcgtcaccgc gccggatggc aatcacacag tctatattcg cgacaacggg
    11221 attggcatcg gtgaaccgaa agaacccgaa ggccattatg gtctgaatat catgcgcgaa
    11281 cgcgcggaaa gattgggtgg aacgctgact ttttcacaac cttccggcgg cggcacgtta
    11341 gtgagtatta gctttcgctc tgcggagggt gaggaaagtc agctgatgta atgcctctta
    11401 ttgaccaaag aatagtggca cttaaggttc agtataaaag ggcatgataa tttacattaa
    11461 ctcctttttt tctccacgat tggctcgtac cttgccgcta cagtgaagca agtcaagcct
    11521 acaacgatac gcagaaacac gaggtcctct tttaatggcg aatttcttta ttgatcgccc
    11581 catttttgcc tgggtgctgg caatcctgtt gtgtctgaca ggtaccctgg cgattttttc
    11641 attgcccgtt gaacaatacc ccgatctcgc gccaccgaat gtgcgagtga ccgctaacta
    11701 tcccggcgca tcggcccaga cgctggaaaa caccgtgacc caggttatcg agcaaaatat
    11761 gaccggcctc gataatctca tgtatatgtc gtctcagagc agtggcaccg gccaggcatc
    11821 tgtcacttta agttttaaag caggcaccga tccggacgaa gccgtgcagc aagtacaaaa
    11881 ccagctgcaa tcagccatgc gaaagttacc gcaggcggtg caaaatcagg gcgtgacggt
    11941 gcgtaaaacc ggcgatacca atattctgac cattgccttc gtctctaccg atggttcgat
    12001 ggataaacag gatattgccg attacgttgc cagtaatatt caggacccgt taagccgcgt
    12061 gaatggcgtc ggggatatcg atgcctatgg ttcgcaatat tccatgcgta tctggctgga
    12121 cccggcgaaa ctcaacagtt tccagatgac ggctaaagat gtcactgatg ccattgagtc
    12181 acagaacgcg cagattgcgg ttgggcaact tggtggtacg ccttccgtcg ataagcaggc
    12241 gctcaacgcc accattaacg cccagtcact gctgcaaaca ccagaacagt tccgcgatat
    12301 caccttgcgg gtgaatcagg acggttcaga ggtaaggctg ggcgatgtcg ccaccgtcga
    12361 aatgggggcg gaaaaatacg attatcttag ccgcttcaat ggtaagccag cctccgggct
    12421 gggggtaaaa ctggcctccg gcgcgaacga aatggcgact gcgcagctgg tgctcaatcg
    12481 tctcgacgag ctggcgcagt attttccaca cggactggaa tacaaggtgg cgtatgaaac
    12541 cacctcgttt gttaaagcct ccattgaaga cgtggtgaaa acgctgctgg aagctatcgc
    12601 tctggttttc ctcgttatgt atctgttcct gcaaaacttc cgcgccacgc tgatacccac
    12661 tatcgccgtg ccggtggtgt tgatgggaac cttctccgta ctttatgcct tcggttacag
    12721 cgtcaacacc ttaaccatgt tcgcgatggt gctggcgatc ggtctgctgg tggatgatgc
    12781 catcgtggtg gtggaaaacg tcgaacgtat tatgagtgag gaaggactca ctcctcgcga
    12841 agccacacgt aaatcgatgg ggcagatcca gggggcactg gtcgggattg cgatggtgct
    12901 ttcggcggta tttgtgccaa tggccttctt cggcggcacc accggtgcca tctatcgcca
    12961 gttctctatt accattgttg cggcgatggt gctgtcagta ctggtagcga tgatcctcac
    13021 tccggctctg tgtgccacgc tacttaagcc actgaaaaaa ggtgagcatc acgggcaaaa
    13081 aggctttttt gcctggttta accagatgtt taaccgcaac gccgaacgct acgaaaaagg
    13141 ggtggcgaaa attctccacc gtagcctgcg ctggattgtg atttatgtcc tgctgcttgg
    13201 cggcatggtg ttcctgttcc tgcgtttgcc gacgtcgttc ttgccacttg aagaccgtgg
    13261 catgtttact acctcggtac agttgcccag cggttcaacc caacaacaga ccctgaaagt
    13321 cgttgagcaa atcgagaaat actacttcac ccatgaaaaa gacaacatca tgtcggtgtt
    13381 tgccactgtt ggttctggcc ctgggggtaa cggacaaaac gtggcgagaa tgtttatccg
    13441 cctgaaagac tggagcgaac gcgacagtaa gaccggcacc tcgtttgcca ttatcgagcg
    13501 tgcaacgaag gcgtttaaca agattaaaga agctcgcgtt atcgccagca gcccgccagc
    13561 aattagcggt cttggtagtt ctgcaggttt tgatatggag ttgcaggacc acgctggagc
    13621 gggtcacgat gcgctgatgg cagcacgtaa ccagttgctg gcgctggcgg cggaaaaccc
    13681 ggagctaacc cgtgtgcgcc ataacggcct cgacgacagt ccgcagttgc agattgatat
    13741 cgaccagcgt aaagctcagg cgctgggcgt tgctatcgac gatattaacg acacactgca
    13801 aaccgcctgg ggttcgagct atgtgaatga ctttatggat cgcggtcgcg tgaagaaagt
    13861 ctatgtgcag gcagctgcgc cgtatcgcat gctgccagat gacatcaatc tctggtatgt
    13921 ccgaaataaa gatggcggca tggtgccctt ctctgctttc gcgacctcac gctgggagac
    13981 aggctcgccg cgtctggaac gctataacgg ttattctgcg gtagagattg taggggaagc
    14041 cgcaccgggg gtcagtaccg gtacggcgat ggatattatg gaatcgttag tgaagcagtt
    14101 gccaaacggc tttggtctgg agtggacggc gatgtcgtat caggagcggc tttccggcgc
    14161 gcaggctccg gcgctgtacg ccatttcctt gctggtggta ttcctgtgtc ttgcggcgtt
    14221 gtatgaaagc tggtcggtgc cgttctcggt aatgctggtc gtgccgctgg gggttatcgg
    14281 cgcgctgctg gcaacctgga tgcgcgggct ggaaaacgac gtttacttcc aggtgggcct
    14341 gttaacggtc attggtttat cggcgaaaaa cgccatcctg atcgtcgagt ttgctaacga
    14401 gatgaaccaa aaaggccacg acctgtttga agcgacgctc cacgcctgcc gtcagcgttt
    14461 acgcccgatt ctgatgactt cgctggcatt tatcttcggc gtattgccaa tggcaaccag
    14521 cacgggtgcc ggttccggtg gtcagcatgc ggtgggtact ggcgtaatgg gcgggatgat
    14581 ttcggccact attctggcta tttacttcgt gccgctgttc tttgtgctgg tgcgccgccg
    14641 cttcccgctg aagccgcgcc cggaataagc aataaaaaag gcgacatgct aatgtgtcgc
    14701 ctttttcaac tttccgatta agaacctgct cagcgggttc ttgctgtttg tactttgtct
    14761 caggaattac ttacgaagca taacttcgat aaagtctttc cagttcccca gttcacgttc
    14821 aatcataaca acctctctta taattatggg tattctacgg aaacaatata ccgtggtgaa
    14881 gctaatttac tcgattgctg cgatgactac ctccggggga caaaccttat gtaaatacta
    14941 tggtcctgcg gtgataattt gtatgtgata cacagcaaca tttcgagata ttcatacggc
    15001 atctaatact ga
//
LOCUS       DAZFLD010000123        14834 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.124, whole genome shotgun sequence.
ACCESSION   DAZFLD010000123 DAZFLD010000000
VERSION     DAZFLD010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14834
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.124"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            241..2157
                     /gene="dnaK"
                     /locus_tag="ACHKBL_003154"
     CDS             241..2157
                     /gene="dnaK"
                     /locus_tag="ACHKBL_003154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HFO5819989.1"
                     /translation="MGKIIGIDLGTTNSCVAIMDGTTPRVLENAEGDRTTPSIIAYTQ
                     DGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAW
                     VEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEV
                     LATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDI
                     DDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLL
                     DVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAAD
                     NKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLN
                     EDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSTRKQVEEVGDKLPADDKTAIE
                     SALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDD
                     DVVDAEFEEVKDKK"
     gene            2246..3376
                     /gene="dnaJ"
                     /locus_tag="ACHKBL_003155"
     CDS             2246..3376
                     /gene="dnaJ"
                     /locus_tag="ACHKBL_003155"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HFO5819990.1"
                     /translation="MAKQDYYEILGVSKTAEEREIKKAYKRLAMKYHPDRNQGDKEAE
                     AKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQGGMGGGGFGGGADFSDIFGDVFGDIF
                     GGGRGRQRAARGADLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQT
                     CPTCHGSGQVQMRQGFFAVQQTCPHCQGRGTLIKDPCNKCHGHGRVERSKTLSVKIPA
                     GVDTGDRIRLAGEGEAGEHGAPAGDLYVQVQVKQHPIFEREGNNLYCEVPINFAMAAL
                     GGEIEVPTLDGRVKLKVPGETQTGKLFRMRGKGVKSVRGGAQGDLLCRVVVETPVGLN
                     EKQKQLLQELQESFGGPTGEHNSPRSKSFFDGVKKFFDDLTR"
     gene            complement(3480..3632)
                     /gene="mokC"
                     /locus_tag="ACHKBL_003156"
     CDS             complement(3480..3632)
                     /gene="mokC"
                     /locus_tag="ACHKBL_003156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin MokC"
                     /protein_id="HFO5819991.1"
                     /translation="MKQPKAMIVALIVICITAVVAALVTRKDLCEVHIRTGQTEVAVF
                     TAYESE"
     gene            4218..5384
                     /gene="nhaA"
                     /locus_tag="ACHKBL_003157"
     CDS             4218..5384
                     /gene="nhaA"
                     /locus_tag="ACHKBL_003157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HFO5819992.1"
                     /translation="MKHLHRFFSSDASGGIILIIAAILAMIMANSGATSGWYHDFLET
                     PVQLRVGSLEINKNMLLWINDALMAVFFLLVGLEVKRELMQGSLASLRQAAFPVIAAI
                     GGMIVPALLYLAFNHADPITREGWAIPAATDIAFALGVLALLGSRVPLALKIFLMALA
                     IIDDLGAIIIIALFYTNDLSMASLGVAAVAIAVLAVLNLCGVRRTGVYILVGVVLWTA
                     VLKSGVHATLAGVIVGFFIPLKEKHGRSPAKRLEHVLHPWVAYLILPLFAFANAGVSL
                     QGVTLDGLTSILPLGIIAGLLIGKPLGISLFCWLALRLKLAHLPEGTTYQQIMAVGIL
                     CGIGFTMSIFIASLAFGSVDPELINWAKLGILVGSISSAVIGYSWLRVRLRPSV"
     gene            5444..6349
                     /gene="nhaR"
                     /locus_tag="ACHKBL_003158"
     CDS             5444..6349
                     /gene="nhaR"
                     /locus_tag="ACHKBL_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HFO5819993.1"
                     /translation="MSMSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRAL
                     EERLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVA
                     DALSKRLVSSVLDAAVVEDEQIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQE
                     GLFSMKIGECGVSFWCTNPLPEKPFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNV
                     EILGEFDDAALMKAFGATHNAIFVAPSLYANDFYNDDSVVEIGRVENVMEEYHAIFAE
                     RMIQHPAVQRICNTDYSALFTPASK"
     gene            complement(6445..6708)
                     /gene="rpsT"
                     /locus_tag="ACHKBL_003159"
     CDS             complement(6445..6708)
                     /gene="rpsT"
                     /locus_tag="ACHKBL_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HFO5819994.1"
                     /translation="MANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDK
                     AAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA"
     gene            6811..7029
                     /locus_tag="ACHKBL_003160"
     CDS             6811..7029
                     /locus_tag="ACHKBL_003160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2575 family protein"
                     /protein_id="HFO5819995.1"
                     /translation="MCRHSLRSDGAGFYQLAGCEYSFSARKIAAGGQFLPMICAIAMK
                     SHFFLISVLNRRLTLTAVQGILGRFSLF"
     gene            7037..7978
                     /gene="ribF"
                     /locus_tag="ACHKBL_003161"
     CDS             7037..7978
                     /gene="ribF"
                     /locus_tag="ACHKBL_003161"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HFO5819996.1"
                     /translation="MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQEEGR
                     KRNLPVMVMLFEPQPLELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAAL
                     TAQNFISDLLVKHLRVKFLAVGDDFRFGAGREGDFLLLQKAGMEYGFDITSTQTFCEG
                     GVRISSTAVRQALADDNLALAESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQV
                     SPVKGVYAVEVLGLGEKPLPGVANIGTRPTVAGIRQQLEVHLLDVAMDLYGRHIQVVL
                     RKKIRNEQRFASLDELKAQIARDELTAREFFGLTKPA"
     gene            8021..10837
                     /gene="ileS"
                     /locus_tag="ACHKBL_003162"
     CDS             8021..10837
                     /gene="ileS"
                     /locus_tag="ACHKBL_003162"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HFO5819997.1"
                     /translation="MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAA
                     KKGKKTFILHDGPPYANGSIHIGHSVNKILKDIIIKSKGLSGYDSPYVPGWDCHGLPI
                     ELKVEQEYGKPGEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFK
                     TEANIIRALGKIIGNGHLHKGAKPVHWCVDCRSALAEAEVEYYDKTSLSIDVAFQAVD
                     QDALKTKFGVSNVNGPISLVIWTTTPWTLPANRAISIAPDFDYALVQIDGQAVILAKD
                     LVESVMQRIGVSDYTILGTVKGAELELLRFTHPFMDFDVPAILGDHVTLDAGTGAVHT
                     APGHGPDDYVIGQKYGLETANPVGPDGTYLPGTYPTLDGVNVFKANDIVIALLQEKGA
                     LLHVEKMQHSYPCCWRHKTPIIFRATPQWFVSMDQKGLRAQSLKEIKGVQWIPDWGQA
                     RIESMVANRPDWCISRQRTWGVPMSLFVHKDTEELHPRTLELMEEVAKRVEVDGIQAW
                     WDLDAKEILGDEADQYVKVPDTLDVWFDSGSTHSSVVDVRPEFAGHAADMYLEGSDQH
                     RGWFMSSLMISTAMKGKAPYRQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADI
                     LRLWVASTDYTGEMAVSDEILKRAADSYRRIRNTARFLLANLNGFDPAKDMVKPEEMV
                     VLDRWAVGCANAAQEDILKAYEAYDFHEVVQRLMRFCSVEMGSFYLDIIKDRQYTAKA
                     DSVARRSCQTALYHIAEALVRWMAPILSFTADEVWGYLPGEREKYVFTGEWYEGLFGL
                     ADSEAMNDAFWDELLKVRGEVNKVIEQARADKKVGGSLEAAVTLYAEPELAAKLTALG
                     DELRFVLLTSGATVADYNDAPADAQQSEVLKGLKVALSKAEGEKCPRCWHYTQDVGKV
                     AEHAEICGRCVSNVAGDGEKRKFA"
     gene            10837..11331
                     /gene="lspA"
                     /locus_tag="ACHKBL_003163"
     CDS             10837..11331
                     /gene="lspA"
                     /locus_tag="ACHKBL_003163"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HFO5819998.1"
                     /translation="MSQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDTVPLFP
                     SLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGISVILAVMMYRSKATQKLNNIAYA
                     LIIGGALGNLFDRLWHGFVVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSK
                     AKKQ"
     gene            11439..11888
                     /gene="fkpB"
                     /locus_tag="ACHKBL_003164"
     CDS             11439..11888
                     /gene="fkpB"
                     /locus_tag="ACHKBL_003164"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFO5819999.1"
                     /translation="MSESVQSNSAVLVHFTLKLDDGTTAESTRNNGKPALFRLGDASL
                     SEGLEQHLLGLKVGDKTTFSLEPDAAFGVPSPDLIQYFSRREFMDAGEPEIGAIMLFT
                     AMDGSEMPGVIREINGDSITVDFNHPLAGQTVHFDIEVLEIDPALEA"
     gene            11890..12840
                     /gene="ispH"
                     /locus_tag="ACHKBL_003165"
                     /gene_synonym="lytB"
     CDS             11890..12840
                     /gene="ispH"
                     /locus_tag="ACHKBL_003165"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-enyl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HFO5820000.1"
                     /translation="MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRY
                     VVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKV
                     HMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEK
                     LSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVL
                     VVGSKNSSNSNRLAELAQRMGKRAFLIDDAKDIQEEWVKGVNCVGVTAGASAPDILVQ
                     NVVARLQQLGGGEAIPLEGREENIVFEVPKELRVDIREVD"
     gene            12906..13820
                     /gene="rihC"
                     /locus_tag="ACHKBL_003166"
     CDS             12906..13820
                     /gene="rihC"
                     /locus_tag="ACHKBL_003166"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside hydrolase RihC"
                     /protein_id="HFO5820001.1"
                     /translation="MRLPIFLDTDPGIDDAVAIAAAIFTPELDLQLMTTVAGNVSVEK
                     TTRNALQLLHFWNAEIPLAQGAAVPLVRAPRDAASVHGESGMAGYDFVEHNRQPLGIP
                     AFLAIRDALMRAPEPVTLVAIGPLTNIALLLSQCPECKPYIRRLVIMGGSAGRGNCTP
                     NAEFNIAADPEAAACVFRSGIEIVMCGLDVTNQAILTPDYLATLPELNRTGKMLHALF
                     SHYRSGSMQSGLRMHDLCAIAWLVRPDLFTLKPCFVAVETQGEFTSGTTVVDIDGCLG
                     KPANVQVALDLNVKGFQQWVAEVLALVP"
     gene            13987..14808
                     /gene="dapB"
                     /locus_tag="ACHKBL_003167"
     CDS             13987..14808
                     /gene="dapB"
                     /locus_tag="ACHKBL_003167"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HFO5820002.1"
                     /translation="MHDANIRVAIAGAGGRMGRQLIQAALALEGVQLGAALEREGSSL
                     LGSDAGELAGAGKTGVTVQSSLDAIKDDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIG
                     TTGFDEAGKQAIRDAAADIAIVFAANFSVGVNVMLKLLEKAAKVMGDYTDIEIIEAHH
                     RHKVDAPSGTALAMGEAIAHALDKDLKDCAVYSREGHTGERVPGTIGFATVRAGDIVG
                     EHTAMFADIGERLEITHKASSRMTFANGAVRSALWLSGKESGLFDMRDVLDLNNL"
BASE COUNT         3537 a         3611 c         4079 g         3607 t
ORIGIN      
        1 atatgaataa aattgctgtc agtttcatgt cttgttctgg catatcgcga aatttctgcg
       61 caaaagcaca aaaaattttt gcatctcccc cttgatgacg tgggttacga ccccatttag
      121 tagtcaaccg cagtgagtga gtctgcaaaa aaatgaaatt gggcagttga aaccagacgt
      181 ttcgccccta ttacagactc acaaccacat gatgaccgaa tatatagtgg agacgtttag
      241 atgggtaaaa taattggtat cgacctgggt actaccaact cttgtgtagc gattatggat
      301 ggcaccactc ctcgtgtact ggagaacgcc gaaggcgatc gcaccacgcc ttctatcatt
      361 gcctataccc aggatggtga aactctggtt ggtcagccgg ctaaacgtca ggcagtgacg
      421 aacccgcaaa acaccctgtt tgcgattaaa cgcctgattg gccgccgctt ccaggacgaa
      481 gaagtacagc gtgatgtttc catcatgccg ttcaaaatta ttgctgctga taacggcgac
      541 gcatgggtcg aagttaaagg ccagaaaatg gcaccgccgc agatctctgc tgaagtgctg
      601 aaaaaaatga agaaaaccgc tgaagattac ctgggtgaac cggtaactga agctgttatc
      661 accgtaccgg catactttaa cgatgctcag cgtcaggcaa ccaaagacgc aggccgtatc
      721 gctggtctgg aagtaaaacg tatcatcaac gaaccgaccg cagctgcgct ggcttacggt
      781 ctggacaaag gtactggcaa ccgtactatc gcggtttatg acctgggtgg tggtactttc
      841 gatatttcta ttatcgaaat cgacgaagtt gacggcgaaa aaaccttcga agttctggca
      901 accaacggtg atacccacct gggtggtgaa gacttcgaca gccgtctgat caactacctg
      961 gttgaagaat tcaagaaaga tcagggcatt gacctgcgca acgatccgct ggcaatgcag
     1021 cgcctgaaag aagcggcaga aaaagcgaaa atcgaactgt cttccgctca gcagaccgac
     1081 gttaacctgc cgtacatcac tgcagacgcg accggtccga aacacatgaa catcaaagtg
     1141 actcgtgcga aactggaaag cctggttgaa gatctggtaa accgttccat tgagccgctg
     1201 aaagttgcac tgcaggacgc tggcctgtcc gtatctgata tcgacgacgt tatcctcgtt
     1261 ggtggtcaga ctcgtatgcc aatggttcag aagaaagttg ctgagttctt tggtaaagag
     1321 ccgcgtaaag acgttaaccc ggacgaagct gtagcaatcg gtgctgctgt tcagggtggt
     1381 gttctgactg gtgacgtaaa agacgtactg ctgctggacg ttaccccgct gtctctgggt
     1441 atcgaaacca tgggcggtgt gatgacgacg ctgatcgcga aaaacaccac tatcccgacc
     1501 aagcacagcc aggtgttctc taccgctgaa gacaaccagt ctgcggtaac catccatgtg
     1561 ctgcagggtg aacgtaaacg tgcagctgat aacaaatctc tgggtcagtt caacctggat
     1621 ggtatcaacc cggcaccgcg cggcatgccg cagatcgaag ttaccttcga tatcgatgct
     1681 gacggtatcc tgcacgtttc cgcgaaagat aaaaacagcg gtaaagagca gaagatcacc
     1741 atcaaggctt cttctggtct gaacgaagat gaaatccaga aaatggtacg cgacgcagaa
     1801 gctaacgccg aagctgaccg taagtttgaa gagctggtac agactcgcaa ccagggcgac
     1861 catctgctgc acagcacccg taagcaggtt gaagaagtgg gcgacaaact gccggctgac
     1921 gacaaaactg ctatcgagtc tgcactgact gcactggaaa ctgctctgaa aggtgaagac
     1981 aaagccgcta tcgaagcgaa aatgcaggaa ctggcacagg tttcccagaa actgatggaa
     2041 atcgcccagc agcaacatgc ccagcagcag actgccggtg ctgatgcttc tgcaaacaac
     2101 gcgaaagatg acgatgttgt cgacgctgaa tttgaagaag tcaaagacaa aaaataatcg
     2161 ccctataaac gggtaattat actgacacgg gcgaagggga atttcctctc cgcccgtgca
     2221 ttcatctagg ggcaatttaa aaaagatggc taagcaagat tattacgaga ttttaggcgt
     2281 ttccaaaaca gcggaagagc gtgaaatcaa aaaggcctac aaacgcctgg ccatgaaata
     2341 ccacccggac cgtaaccagg gtgacaaaga ggccgaggcg aaatttaaag agatcaagga
     2401 agcttatgaa gttctgaccg actcgcaaaa acgtgcggca tacgatcagt atggtcatgc
     2461 tgcgtttgag caaggtggca tgggaggcgg cggtttcggc ggcggcgcag acttcagcga
     2521 tatttttggt gacgttttcg gcgatatttt tggcggcgga cgtggtcgtc aacgtgcggc
     2581 gcgcggtgct gatttacgct ataacatgga gctcaccctc gaagaagctg tacgtggcgt
     2641 gaccaaagag atccgcattc cgactctgga agagtgtgac gtttgccacg gtagcggtgc
     2701 aaaaccaggt acacagccgc agacctgtcc gacctgtcat ggttctggtc aggtgcagat
     2761 gcgccagggt ttctttgccg tgcagcagac ctgtccacac tgtcagggcc gcggtacgct
     2821 gatcaaagat ccgtgcaaca aatgtcatgg tcatggtcgt gttgagcgca gcaaaacgct
     2881 gtccgttaaa atcccggcag gggtggacac tggagaccgc atccgtcttg cgggcgaagg
     2941 tgaagcgggc gagcatggcg caccggcagg cgatctgtac gttcaggttc aggttaaaca
     3001 gcacccgatt ttcgagcgtg aaggcaacaa cctctattgc gaagtcccga tcaacttcgc
     3061 tatggcggcg ctgggtggcg aaatcgaagt accgaccctt gatggtcgcg tcaaactgaa
     3121 agtgcctggc gaaacccaga ccggtaagct gttccgtatg cgcggtaaag gcgtcaagtc
     3181 tgtccgcggt ggcgcacagg gtgatttgct gtgccgcgtt gtcgtcgaaa caccggtagg
     3241 cctgaacgag aagcagaaac agctgctgca agagctgcaa gaaagcttcg gtggcccaac
     3301 cggcgagcac aacagcccgc gctcaaagag cttctttgat ggtgtgaaga agttttttga
     3361 cgacctgacc cgctaatctc cccaaaagcc tgcccgtggg caggcctggg taaaaatagg
     3421 gtgcgttgaa gatatgcgag cacctgaaaa gtggcgggga tcactccccg ccgttgctct
     3481 tactcggatt cgtaagccgt gaaaacagca acctccgtct ggccagttcg gatgtgaacc
     3541 tcacagaggt cttttctcgt taccagcgcc gccactacgg cggtgataca gatgacgatc
     3601 agggcgacaa tcatcgcctt aggctgcttc attgctctct tctccttgac cttacggtca
     3661 gtaagaggca ctctacatgt gttctgcata tagggggcct cgggttgata gtaaaatatc
     3721 actcggggct tttctctatc tgccgttcag ctaatgcctg agacagacag cctcaagcac
     3781 ccgccgctat tatatcgctc tctttaaccc attctgtttt atcgattcta atcctgaaga
     3841 cgcctcgcat ttttatggcg taatttttta atgatttaat tatttaactt taatttatct
     3901 cttcatcgca attattgacg acaaactgga ttatttttga agtattggcc taacaaacat
     3961 cgccgactga caacaaatta attattactt ttcctaatta atccctcaga aatcctgacc
     4021 ttaagctatg attatcaggg cttagggtca cacgtcagcg cttacagccg tcaaaaactc
     4081 atctcatcac tgatgacgca aatccttcaa tgcctcgtaa aaagcgaatt attcctacac
     4141 tataatctga ttttaacgat gattcgtgcg gggtaagata gtaaaaacga tctattcacc
     4201 tgaaagagaa ataaaaagtg aaacatctgc atcgattctt tagcagtgat gcctcgggag
     4261 gcattattct tatcattgcc gctatcctgg cgatgattat ggccaacagc ggcgcaacca
     4321 gtggatggta tcacgacttt ctggagacgc cggttcagct ccgggttggt tcactcgaaa
     4381 tcaacaaaaa catgctgtta tggataaatg acgcgctgat ggcggtattt ttcctgttag
     4441 tcggtctgga agttaaacgt gaactgatgc aaggatcgct agccagctta cgccaggctg
     4501 catttccagt tatcgccgct attggtggga tgattgtgcc ggcattactc tatctggcct
     4561 ttaaccatgc cgatccgatt acccgcgaag ggtgggcgat cccggcggct actgacattg
     4621 cttttgcact tggtgtgctg gcgctgttgg gaagtcgtgt tccgttagcc ctgaagatct
     4681 ttttgatggc tctggctatt atcgacgatc ttggggccat cattatcatc gcattgttct
     4741 acactaatga cttatcgatg gcctctcttg gcgtcgcggc tgtagcaatt gcggtactcg
     4801 cggtattgaa tctgtgtggt gtacgccgca cgggcgtcta tattctggtt ggcgtggtgt
     4861 tgtggactgc ggtgttgaaa tcgggggttc acgcaaccct ggcgggggta attgtcggct
     4921 tctttattcc tttgaaagag aagcatgggc gttctccagc gaagcgactg gagcatgtgt
     4981 tgcacccgtg ggtggcgtat ctgattttac cgctgtttgc atttgctaat gctggcgttt
     5041 cactgcaagg cgtcacgctg gatggcttga cctccattct gccattgggg atcatcgctg
     5101 gcttgctgat tggcaaaccg ctggggatta gtctgttctg ctggttggcg ctgcgtttga
     5161 aactggcgca tctgcctgag ggaacgactt atcagcaaat tatggcggtg gggatcctgt
     5221 gcggtatcgg ttttactatg tctatcttta ttgccagcct ggcctttggt agcgtagatc
     5281 cagaactgat taactgggcg aaactcggta tcctggtcgg ttctatctct tcggcggtaa
     5341 ttggatacag ctggttacgc gttcgtttgc gtccatcagt ttgacaggac ggtttaccgg
     5401 ggagccataa acggctccct tttcattgtt atcagggaga gaaatgagca tgtctcatat
     5461 caattacaac cacttgtatt acttctggca tgtctataaa gaaggttccg tggttggcgc
     5521 agcggaggcg ctttatttaa caccacaaac cattaccgga cagattcggg cgctggaaga
     5581 gcgcctgcaa ggcaaattat ttaagcgcaa gggacgtggc ctcgagccca gcgagctggg
     5641 agagctggtc tatcgctatg ccgataaaat gttcacctta agccaggaaa tgctggatat
     5701 cgtcaactat cgcaaagagt ccaacttatt gtttgatgtt ggtgtggcag atgcactttc
     5761 caaacgtctg gtcagcagtg ttctggatgc cgcagttgtg gaagacgagc agatccatct
     5821 acgctgtttc gaatcgacgc acgagatgct tttagagcag ttgagtcagc ataaactgga
     5881 tatgatcatc tctgactgtc cgatcgattc cactcagcag gaagggctgt tttccatgaa
     5941 aattggcgaa tgtggtgtca gtttctggtg cactaaccca ctaccagaaa agccgtttcc
     6001 tgcctgtctt gaagagcgtc gtttacttat tccggggcgt cgctcaatgt tggggcgtaa
     6061 gctattaaac tggtttaact cccagggctt gaacgtcgaa attttgggtg agtttgatga
     6121 tgctgcgttg atgaaagcct ttggggcgac gcataacgct attttcgttg caccttcgct
     6181 ttacgctaat gatttctata acgatgactc ggttgtggag ataggccgtg ttgagaacgt
     6241 gatggaagag taccacgcga tttttgccga aaggatgatt cagcaccctg cagtacagcg
     6301 tatctgcaat acagactatt ctgcgctgtt tactccagct tcaaaataaa catttgtact
     6361 ttgcaggccg aattttggcc tgcagatgga tataaaaaaa cccgccagag cgggtttttt
     6421 tacaaagctg acaacaacag gcaattaagc cagtttgttg atctgtgcag tcaggttagc
     6481 cttatgacgt gcagctttgt ttttgtggat cagaccttta gcagcctgac ggtccacgat
     6541 tggttgcatt tcgttaaatg ctttctgtgc agcagctttg tcgccagctt cgatagctgc
     6601 gtatactttc ttgatgaaag tacgcatcat agagcgacgg cttgcgttgt gcttacgagc
     6661 cttttcagac tgaatggcgc gcttcttagc tgatttgata ttagccaagg tccaactccc
     6721 aaatgtgttc tatatggaca attcaaaggc cgaggaatat gcccttttag ccttcttttg
     6781 tcaatggatt tgtgcaaata agcgccgtta atgtgccggc actcgttacg tagtgatggc
     6841 gcaggattct accagcttgc ggggtgtgaa tacagctttt ccgcgagaaa aattgcagca
     6901 ggcggtcagt ttcttcccat gatttgcgcc atagcaatga aaagccactt ctttctgatt
     6961 tcggtactca atcgccggtt aaccttgacc gctgtacaag gtatactcgg acgattttca
     7021 ctgttttgag ccagacatga agctgatacg cggcatacat aatctcagcc aggccccgca
     7081 agaagggtgt gtgctgacta ttggtaattt cgacggcgtg catcgcggtc atcgcgcgct
     7141 gttacagggc ttgcaggaag aagggcgcaa gcgcaactta ccggtgatgg tgatgctttt
     7201 tgaacctcaa ccactggaac tgtttgctac tgataaagcc ccggcacggc tcacccggct
     7261 gcgggaaaaa ctgcgttatc ttgcagagtg tggtgttgat tacgtgctgt gcgtgcgttt
     7321 tgacaggcgt tttgcggcgt taaccgcgca aaacttcatc agtgatcttc tggtgaagca
     7381 cttgcgggta aaatttcttg ccgtaggtga cgatttccgc tttggcgctg gtcgtgaagg
     7441 cgatttcttg ttattacaga aagcgggcat ggaatacggc ttcgatatca ccagcacgca
     7501 aactttttgc gaaggtggcg tgcgtatcag cagcaccgcc gtgcgtcagg cgcttgcgga
     7561 tgacaatctg gctctggcag aaagtttact ggggcacccg tttgctatct ccgggcgtgt
     7621 agtccacggt gatgaattag ggcgcactat aggtttcccg acggcgaatg taccgctacg
     7681 ccgtcaggtt tccccggtga aaggggttta tgcggtagaa gtgttgggcc ttggcgaaaa
     7741 gccgttaccc ggcgttgcaa acatcggaac acgcccaacg gttgccggta ttcgccagca
     7801 actggaagtg catttgttag atgttgcaat ggacctttat ggtcgccata tacaagtagt
     7861 gctgcgtaaa aaaatacgca atgagcagcg atttgcatcg ctggacgaac tgaaagcgca
     7921 gattgcgcgt gatgaattaa ccgcccgcga attttttggg ctaacaaaac cggcttaagc
     7981 ctgttatgta atcaaaccga aatacggaac cgagaatctg atgagtgact ataaatcaac
     8041 cctgaatttg ccggaaacag ggttcccgat gcgtggcgat ctcgccaagc gcgaaccggg
     8101 aatgctggcg cgttggactg atgatgatct gtacggcatc attcgtgcag ctaaaaaagg
     8161 caaaaaaacc ttcattctgc atgatggccc tccttatgcg aatggcagta ttcatattgg
     8221 tcactcggtt aacaagattc tgaaagacat tatcattaag tccaaagggc tttctggata
     8281 tgactcgccg tatgtgcctg gctgggactg tcatggtctg ccaatcgagc tgaaagtaga
     8341 gcaagaatac ggtaagccgg gggagaaatt caccgccgct gagttccgcg ccaagtgccg
     8401 cgaatacgct gcgacccagg ttgacggtca gcgcaaagac tttatccgtc tgggcgtgct
     8461 gggcgactgg tcgcacccgt acctgaccat ggacttcaaa actgaagcca acatcatccg
     8521 cgcgctgggc aaaatcatcg gcaacggtca cctgcacaaa ggcgcgaagc cggtgcactg
     8581 gtgcgttgac tgccgttctg cactggcaga agcggaagtt gagtattacg acaaaacttc
     8641 tctgtccatc gacgtcgctt tccaggcggt cgatcaggat gcgcttaaaa cgaaatttgg
     8701 cgtaagcaat gttaacggcc caatttcgct ggttatatgg accaccacgc cgtggacgct
     8761 gcctgctaac cgcgcaatct ccatcgcacc tgattttgat tatgcgctgg tgcaaatcga
     8821 cggtcaggcc gtgatcctcg cgaaagatct ggttgaaagc gtaatgcagc gtatcggcgt
     8881 tagcgattac accattcttg gcacggtgaa aggtgccgag ctggaactgt tgcgctttac
     8941 ccatccgttt atggacttcg atgttccggc aattctcggc gaccacgtta cgctggatgc
     9001 cggtaccggt gccgttcata ccgcgccagg ccacggtccg gacgactatg tgatcggtca
     9061 aaaatatggt ctggaaaccg ctaacccggt tggcccggac ggcacttatc tgccgggtac
     9121 ttacccgact ctggatggcg ttaacgtctt caaagcgaac gatattgtca ttgcgttgtt
     9181 gcaggaaaaa ggcgcactgt tgcacgttga gaaaatgcaa cacagctatc cgtgctgctg
     9241 gcgtcataaa acgccgatca tcttccgcgc gacgccgcag tggttcgtca gcatggatca
     9301 gaaaggtctg cgtgcgcagt cactgaaaga gatcaaaggc gtgcagtgga tccctgactg
     9361 gggccaggcg cgtatcgagt cgatggttgc taaccgtcct gactggtgta tctctcgtca
     9421 gcgtacctgg ggcgtgccga tgtcactgtt cgtgcacaaa gacacagaag aactgcatcc
     9481 gcgtactctc gaactgatgg aagaagtggc aaaacgcgtt gaagttgacg gcattcaggc
     9541 gtggtgggat ctcgatgcga aagagatcct cggcgacgaa gctgaccagt atgtgaaagt
     9601 accggatacg ctggatgtat ggtttgactc cggatctacc cactcttccg ttgttgatgt
     9661 gcgtccggaa tttgccggtc acgcagcgga catgtatctg gaaggttctg accaacaccg
     9721 tggctggttc atgtcttccc tgatgatctc taccgcgatg aagggcaaag cgccatatcg
     9781 tcaggtactg actcacggct ttaccgtgga tggtcagggt cgcaagatgt ctaaatccat
     9841 cggtaacacc gtttcgccgc aggatgtgat gaacaaactg ggtgcggata ttctgcgtct
     9901 gtgggtggca tcaaccgact acactggcga aatggccgtt tctgacgaga tcctgaaacg
     9961 tgctgccgac agctatcgtc gtatccgtaa caccgcgcgc ttcctgctgg caaacctgaa
    10021 cggttttgat ccggcaaaag atatggtgaa accggaagag atggtggtac tggatcgctg
    10081 ggccgtaggt tgtgcgaacg cggcacagga agacatcctc aaagcgtacg aagcatacga
    10141 tttccacgaa gtggtacagc gtctgatgcg cttctgctcc gttgagatgg ggtccttcta
    10201 cctcgacatc atcaaagacc gtcagtatac cgccaaagcg gacagcgtgg cgcgtcgtag
    10261 ctgccagact gcgctgtatc acatcgcaga agcgctggtt cgctggatgg caccaatcct
    10321 ctccttcacc gctgatgaag tgtggggtta cctgccgggc gaacgtgaaa aatacgtctt
    10381 caccggtgag tggtacgaag gcctgtttgg tctggcagac agtgaagcaa tgaacgatgc
    10441 gttctgggac gagctgttga aagtgcgtgg cgaagtgaac aaagtcattg agcaagcgcg
    10501 tgccgataag aaagtgggcg gctcgctgga agcggcagta accttgtatg cagaaccgga
    10561 actggcggcg aaactgaccg cgctgggcga tgaattacga tttgtcctgt tgacctccgg
    10621 cgctaccgtt gcagactata acgacgcacc tgctgacgcc cagcagagcg aagtcctcaa
    10681 agggctgaaa gtcgcgttga gtaaagccga aggtgagaag tgcccacgct gctggcacta
    10741 cacccaggat gtcggcaagg tggcggaaca cgcagaaatc tgcggccgtt gtgtcagcaa
    10801 cgtcgccggt gacggtgaaa aacgtaagtt tgcctgatga gtcaatcgat ctgttcaaca
    10861 gggctacgct ggctgtggct ggtggtagtc gtgctgatta tcgatctggg cagcaaatac
    10921 ctgatcctcc agaactttgc tctgggggat acggtcccgc tgttcccgtc gcttaatctg
    10981 cattatgcgc gtaactatgg cgcggcgttt agtttccttg ccgatagtgg cggctggcag
    11041 cgttggtttt ttgccggtat tgcgattggt attagcgtga tcctggcagt gatgatgtat
    11101 cgctcgaagg ccacgcagaa gctaaacaat atcgcttacg cgctgattat tggcggcgcg
    11161 ctgggcaacc tgttcgaccg cctgtggcac ggcttcgttg tcgatatgat cgacttctat
    11221 gtcggcgact ggcacttcgc caccttcaac cttgccgata ctgccatttg tgtcggtgcg
    11281 gcactgattg tgctggaagg ttttttacct tctaaagcga aaaaacaata ataaaccctg
    11341 ccggatgcga tgctgacgca tcttatccgg cctacagatt gctgcgaaat cgtaggccgc
    11401 atccggcaaa aatcctcaaa atataagagc aaacctgcat gtctgaatct gtacagagca
    11461 atagcgccgt cctggtgcac ttcacgctga aactcgatga tggcaccacc gctgagtcta
    11521 cccgcaacaa cggtaaaccg gcgctgttcc gcctgggtga tgcttctctt tctgaagggc
    11581 tggagcaaca cctgctgggg ctgaaagtgg gcgataaaac caccttctcg ctggagcctg
    11641 atgcggcgtt tggcgtgccg tcaccggacc tgattcagta cttctcccgc cgtgaattta
    11701 tggatgcagg cgagccagaa attggcgcaa tcatgctttt taccgcaatg gatggcagtg
    11761 agatgcctgg cgtgatccgc gaaattaacg gcgactccat taccgttgat ttcaaccatc
    11821 cgctggccgg gcagaccgtt cattttgata ttgaagtgct ggaaatcgat ccggcactgg
    11881 aggcgtaaca tgcagatcct gttggccaac ccgcgtggtt tttgtgccgg ggtagaccgc
    11941 gctatcagca ttgttgaaaa cgcgctggcc atttacggcg caccgatata tgtccgtcac
    12001 gaagtggtgc ataaccgcta cgtggtcgat agcttgcgcg agcgtggggc tatctttatt
    12061 gagcagatta gcgaagtacc ggacggcgcg atcctgattt tctccgcaca cggtgtttct
    12121 caggcagtac gtaacgaagc gaaaagccgc gatttgacgg tgtttgatgc cacctgtccg
    12181 ctggtgacca aagtgcatat ggaagtcgcc cgcgccagcc gtcgtggtga agaatctatt
    12241 ctcatcggtc acgccgggca cccggaagtg gaagggacga tgggccagta cagcaacccg
    12301 gaagggggaa tgtatctggt cgaatcgcca gacgatgtgt ggaaactgac ggtcaaaaac
    12361 gaagagaagc tctcctttat gacccaaacc acgctgtcgg tggatgacac gtctgatgtg
    12421 atcgacgcgc tgcgtaaacg cttcccgaaa attgtcggtc cgcgcaaaga tgacatctgc
    12481 tacgccacga ctaaccgtca ggaggcggta cgcgccctgg cagagcaggc ggaagtagta
    12541 ctggtggttg gttcgaaaaa ctcctccaac tccaaccgtc tggcggaact ggcccagcgt
    12601 atgggcaaac gcgcattttt gattgacgat gcgaaagata tccaggaaga gtgggtaaaa
    12661 ggcgtgaact gcgttggcgt gactgcgggc gcatcggctc cggatattct ggtgcagaat
    12721 gtggtggcac gtttgcaaca gctgggcggt ggtgaagcca ttccgctgga aggtcgtgaa
    12781 gaaaacattg ttttcgaagt gccgaaagag ctgcgtgtcg atattcgtga agtcgattaa
    12841 gtcattagca gcctaagtta tgcgaaaatg ccggtcttgt taccggcatt ttttatggag
    12901 aaaacatgcg tttacctatc ttcctcgata cagaccccgg cattgacgat gccgttgcca
    12961 ttgccgccgc gatttttacc cccgaactcg atctgcaact gatgaccact gtcgcgggta
    13021 atgtttcggt agagaaaacg acccgcaacg ccctgcaact gctgcatttc tggaatgcgg
    13081 agattccgct tgcccagggc gctgccgtac cactggttcg tgcgccgcgc gatgcggcat
    13141 ctgtacacgg tgaatcggga atggctggct atgactttgt tgaacacaac cgtcagccac
    13201 tgggtatacc ggcgtttctg gcaattcgtg atgccctgat gcgtgcgcca gagcccgtta
    13261 ccctggtggc catcggcccg ttaaccaata ttgcgctgtt actttcacaa tgcccggaat
    13321 gtaagccgta tattcgccgt ctggtgatca tgggcggttc tgctggtcgc ggcaactgca
    13381 caccaaacgc cgagtttaat atcgctgccg atccagaagc ggcagcctgt gtcttccgta
    13441 gcggcatcga aatcgtcatg tgcggtctgg atgtcaccaa tcaggcaata ttaacccctg
    13501 actatctcgc taccttgccg gagttgaatc gcaccggaaa aatgctccac gccctgttta
    13561 gtcactaccg tagcggcagt atgcaaagtg gactgcgaat gcacgatctc tgcgccatcg
    13621 cctggctggt gcgcccggac ctgttcactc tcaagccgtg ttttgtggca gtggaaacgc
    13681 agggcgaatt tacctctggc acgacggtgg ttgatatcga cggttgcctg ggcaaaccag
    13741 ccaatgtaca ggtggcattg gatctgaatg taaaaggctt ccagcagtgg gtagctgagg
    13801 tgctggctct ggtgccgtaa cctgtcacat gttatcggca tgcagtcatt catcgactca
    13861 tgcctttcac tgatatccct ccctgtttat cattaatttc taattatcag cgtttttggc
    13921 tggcggcgta gcgatgcgct ggttactctg aaaacggtct atgcaaatta acaaaagaga
    13981 atagctatgc atgatgcaaa catccgcgtt gccatcgcgg gagccggggg ccgtatgggc
    14041 cgccagttga ttcaggcggc gctggcatta gaaggcgtgc agctgggcgc ggcgctggag
    14101 cgcgaaggat cttctttact gggcagcgat gcgggcgaac tggccggagc cgggaaaaca
    14161 ggcgttactg tgcaaagtag tcttgatgcg ataaaagatg attttgatgt gtttatcgat
    14221 tttacccgtc cggaaggtac gctgaaccat ctcgcttttt gtcgccagca tggcaaaggg
    14281 atggtgatcg gcactacggg gtttgacgaa gccggtaaac aggcgattcg tgacgccgcc
    14341 gccgatattg cgattgtctt tgccgccaat tttagcgttg gcgttaacgt catgcttaaa
    14401 ctgctggaga aagcagccaa agtgatgggt gactacaccg atatcgaaat tattgaagca
    14461 catcatagac ataaagttga tgcgccgtca ggcaccgcac tggcaatggg agaggcgatc
    14521 gcccacgctc tggataaaga tctgaaagat tgcgcggtct acagtcgtga aggccatacc
    14581 ggtgaacgtg tgcctggcac cattggtttt gccaccgtgc gtgcaggtga catcgttggt
    14641 gaacataccg cgatgtttgc cgatattggc gagcgtctgg agatcaccca taaggcgtcc
    14701 agccgcatga catttgctaa cggtgcggta agatcggctt tgtggttgag tggtaaggaa
    14761 agtggtcttt ttgatatgcg agatgtgctt gatctcaata atctgtaacc acaaaatatt
    14821 tatagtgatg caaa
//
LOCUS       DAZFLD010000124        14656 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.125, whole genome shotgun sequence.
ACCESSION   DAZFLD010000124 DAZFLD010000000
VERSION     DAZFLD010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14656
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.125"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            289..1578
                     /gene="sdaC"
                     /locus_tag="ACHKBL_003168"
     CDS             289..1578
                     /gene="sdaC"
                     /locus_tag="ACHKBL_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease SdaC"
                     /protein_id="HFO5820003.1"
                     /translation="METTQTSTIASKDSRSAWRKTDTMWMLGLYGTAIGAGVLFLPIN
                     AGVGGMIPLIIMAILAFPMTFFAHRGLTRFVLSGKNPGEDITEVVEEHFGIGAGKLIT
                     LLYFFAIYPILLVYSVAITNTVESFMSHQLGMTPPPRAILSLILIVGMMTIVRFGEQM
                     IVKAMSILVFPFVGVLMLLALYLIPQWNGAALETLSLDTASATGNGLWMTLWLAIPVM
                     VFSFNHSPIISSFAVAKREEYGDMAEQKCSKILAFAHIMMVLTVMFFVFSCVLSLTPA
                     DLAAAKEQNISILSYLANHFNAPIIAWMAPIIAIIAITKSFLGHYLGAREGFNGMVIK
                     SLRGKGKSIEINKLNRITALFMLVTTWIVATLNPSILGMIETLGGPIIAMILFLMPMY
                     AIQKVPAMRKYSGHISNVFVVVMGLIAISAIFYSLFS"
     gene            1636..3003
                     /gene="sdaB"
                     /locus_tag="ACHKBL_003169"
     CDS             1636..3003
                     /gene="sdaB"
                     /locus_tag="ACHKBL_003169"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase II"
                     /protein_id="HFO5820004.1"
                     /translation="MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIARNLLKDVTR
                     VVVDVYGSLSLTGKGHHTDIAIIMGLAGNLPDTVDIDSIPGFIQDVNTHGRLMLANGQ
                     HEVEFPVDQCMNFHADNLSLHENGMRITALAGDKVVYSQTYYSIGGGFIVDEEHFGQQ
                     DSAPVEVPYPYSSAADLQKHCQETGLSLSGLMMKNELALHSKEELEQHLANVWEVMRG
                     GIERGISTEGVLPGKLRVPRRAAALRRMLVSQDKTTTDPMAVVDWINMFALAVNEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFIREVNANSLARYLLVASAIGSLYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGASPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPC
                     IERNAIAAVKAVNAARMALRRTSEPRVCLDKVIETMYETGKDMNAKYRETSRGGLAMK
                     IVACD"
     gene            3114..3869
                     /gene="xni"
                     /locus_tag="ACHKBL_003170"
     CDS             3114..3869
                     /gene="xni"
                     /locus_tag="ACHKBL_003170"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0017108 - 5'-flap endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0033567 - DNA replication, Okazaki fragment
                     processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease Xni"
                     /protein_id="HFO5820005.1"
                     /translation="MAVHLLIVDALNLIRRIHAVQGSPCVETCQHALDQLIMHSQPTH
                     AVAVFDDENRSSGWRHQRLPDYKAGRPPMPEELHDEMPALRAAFEQRGVPCWSASGNE
                     ADDLAATLAVKVTQAGHQATIVSTDKGYCQLLSPTLRIRDYFQKRWLDAPFIDKEFGV
                     QPQQLPDYWGLAGISSSKVPGVVGIGPKSATQLLVEFKSLEGIYENLNAVAEKWRKKL
                     ETHKEMAFLCRDIARLQTDLHIDGNLQQLRLVR"
     gene            complement(3924..5072)
                     /gene="fucO"
                     /locus_tag="ACHKBL_003171"
     CDS             complement(3924..5072)
                     /gene="fucO"
                     /locus_tag="ACHKBL_003171"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HFO5820006.1"
                     /translation="MANRMILNETAWFGRGAVGSLTDEVKRRGYQKALIVTDKTLVQC
                     GVVAKVTDKMDAAGLAWAIYDGVVPNPTITVVKEGLGVFQNSGADYLIAIGGGSPQDT
                     CKAIGIISNNPEFADVRSLEGLSPTNKPSVPILAIPTTAGTAAEVTINYVITDEEKRR
                     KFVCVDPHDIPQVAFIDADMMDGMPPALKAATGVDALTHAIEGYITRGAWALTDALHI
                     KAIEIIAGALRGSVAGDKDAGEEMALGQYVAGMGFSNVGLGLVHGMAHPLGAFYNTPH
                     GVANAILLPHVMRYNADFTGEKYRDIARVMGVKVEGMSLEEARNAAVEAVFALNRDVG
                     IPPHLRDVGVRKEDIPTLAQAALDDVCTGGNPREATLEDIVELYHTAW"
     gene            complement(5100..5747)
                     /gene="fucA"
                     /locus_tag="ACHKBL_003172"
     CDS             complement(5100..5747)
                     /gene="fucA"
                     /locus_tag="ACHKBL_003172"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008738 - L-fuculose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006004 - fucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HFO5820007.1"
                     /translation="MERNKLARQIIDTCLEMTRLGLNQGTAGNVSVRYQDGMLITPTG
                     IPYEKLTESHIVFIDGNGKHEEGKLPSSEWRFHMAAYQSRPDANAVVHNHAVHCTAVS
                     ILNRPIPAIHYMIAAAGGNSIPCAPYATFGTRELSEHVALALKNRKATLLQHHGLIAC
                     EANLEKALWLAHEVEVLAQLYLTTLAITDPVPVLSDEEIAVVLEKFKTYGLRIEE"
     gene            6294..7610
                     /gene="fucP"
                     /locus_tag="ACHKBL_003173"
     CDS             6294..7610
                     /gene="fucP"
                     /locus_tag="ACHKBL_003173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015150 - fucose transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015756 - fucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HFO5820008.1"
                     /translation="MGNTSIQTQSYRAVDKDAGQSRSYIIPFALLCSLFFLWAVANNL
                     NDILLPQFQQAFTLTNFQAGLIQSAFYFGYFIIPIPAGILMKKLSYKAGIITGLFLYA
                     LGAALFWPAAEIMNYTLFLVGLFIIAAGLGCLETAANPFVTVLGPESSGHFRLNLAQT
                     FNSFGAIIAVVFGQSLILSNVPHQSQDVLDKMSPEQLSAYKHSLVLSVQTPYMIIVAI
                     VLLVALLIMLTKFPALQSDNHSDAKQGSFSASLSRLARIRHWRWAVLAQFCYVGAQTA
                     CWSYLIRYAVEEIPGMTAGFAANYLTGTMVCFFIGRFTGTWLISRFAPHKVLAAYALI
                     AMALCLISAFAGGHVGLIALTLCSAFMSIQYPTIFSLGIKNLGQDTKYGSSFIVMTII
                     GGGIVTPVMGFVSDAAGNIPTAELIPALCFAVIFIFARFRSQTATN"
     gene            7643..9418
                     /gene="fucI"
                     /locus_tag="ACHKBL_003174"
     CDS             7643..9418
                     /gene="fucI"
                     /locus_tag="ACHKBL_003174"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008736 - L-fucose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019317 - fucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HFO5820009.1"
                     /translation="MKKISLPKIGIRPVIDGRRMGVRESLEEQTMNMAKATAALLTEK
                     LRHACGAAVECVISDTCIAGMAEAAACEEKFSSQNVGLTITVTPCWCYGSETIDMDPT
                     RPKAIWGFNGTERPGAVYLAAALAAHSQKGIPAFSIYGHDVQDADDTSIPADVEEKLL
                     RFARAGLAVASMKGKSYLSLGGVSMGIAGSIVDHNFFESWLGMKVQAVDMTELRRRID
                     QKIYDEAELEMALAWADKNFRYGEDENNKQYQRNAEQSRAVLRESLLMAMCIRDMMQG
                     NSKLADIGRVEESLGYNAIAAGFQGQRHWTDQYPNGDTAEAILNSSFDWNGVRKPFVV
                     ATENDSLNGVAMLMGHQLTGTAQIFADVRTYWSPEAIERVTGHKLDGLAEHGIIHLIN
                     SGSAALDGSCKQRDSEGKPTMKPHWEISQQEADACLAATEWCPAIHEYFRGGGYSSRF
                     LTEGGVPFTMTRVNIIKGLGPVLQIAEGWSVELPKDVHDILNKRTNSTWPTTWFAPRL
                     TGKGPFTDVYSVMANWGANHGVLTIGHVGADFITLASMLRIPVCMHNVEETKVYRPSA
                     WAAHGMDIEGQDYRACQNYGPLYKR"
     gene            9527..10945
                     /gene="fucK"
                     /locus_tag="ACHKBL_003175"
     CDS             9527..10945
                     /gene="fucK"
                     /locus_tag="ACHKBL_003175"
                     /EC_number="2.7.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008737 - L-fuculokinase activity
                     [Evidence IEA];
                     GO_process: GO:0042355 - L-fucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
                     /protein_id="HFO5820010.1"
                     /translation="MKQEVILVLDCGATNVRAIAVNRQGKIVARASTPNASDIAMENN
                     TWHQWSLDAILQRFADCCRQINSELTDCHIRGIAVTTFGVDGALVDKQGNLLYPIISW
                     KCPRTAAVMDNIERLISAQQLQAISGVGAFSFNTLYKLVWLKENHPQLLERAHAWLFI
                     SSLINHRLTGEFTTDITMAGTSQMLDIQQRDFSPQILQATGIPRRLFPRLVEAGEQIG
                     TLQNSAAAMLGLPVGIPVISAGHDTQFALFGAGAEQNEPVLSSGTWEILMVRSAQVDT
                     SLLSQYAGSTCELDSQAGLYNPGMQWLASGVLEWVRKLFWTAETPWQILIEEARLIAP
                     GADGVKMQCDLLSCQNAGWQGVTLNTTRGHFYRAALEGLTTQLQRNLQMLEKIGHFKA
                     SELLLVGGGSRNTLWNQIKANMLDIPVKVLDDAETTVAGAALFGWYGVGEFNSPEEAR
                     AQIHYQYRYFYPQTEPEFIEEV"
     gene            10947..11369
                     /gene="fucU"
                     /locus_tag="ACHKBL_003176"
     CDS             10947..11369
                     /gene="fucU"
                     /locus_tag="ACHKBL_003176"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016854 - racemase and epimerase activity
                     [Evidence IEA];
                     GO_function: GO:0042806 - fucose binding [Evidence IEA];
                     GO_process: GO:0042354 - L-fucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HFO5820011.1"
                     /translation="MLKTISPLISPELLKVLAEMGHGDEIIFSDAHFPAHSMGPQVIR
                     ADGLLVSDLLQAIIPLFELDSYAPPLVMMAAVEGDTLDPEVERRYRNALSLQAPCPDI
                     IRINRFAFYERAQKAFAIVITGERAKYGNILLKKGVTP"
     gene            11427..12158
                     /gene="fucR"
                     /locus_tag="ACHKBL_003177"
     CDS             11427..12158
                     /gene="fucR"
                     /locus_tag="ACHKBL_003177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose operon activator"
                     /protein_id="HFO5820012.1"
                     /translation="MKAARQQAIVDLLLNHTSLTTEALSEQLKVSKETIRRDLNELQT
                     QGKILRNHGRAKYIHRQNQDSGDPFHIRLKSHYAHKADIAREALAWIEEGMVIALDAS
                     STCWYLARQLPDINIQVFTNSHPICHELGKRERIQLISSGGTLERKYGCYVNPSLISQ
                     LKSLEIDLFIFSCEGIDSSGALWDSNAINADYKSMLLKRAAQSLLLIDKSKFNRSGEA
                     RIGHLDEVTHIISDERQVAASLVTA"
     gene            complement(12202..13302)
                     /gene="rlmM"
                     /locus_tag="ACHKBL_003178"
     CDS             complement(12202..13302)
                     /gene="rlmM"
                     /locus_tag="ACHKBL_003178"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010435097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HFO5820013.1"
                     /translation="MNKVVLLCRPGFEKECAAEITDKAGQREIFGFARVKENAGYVIY
                     ECYQPDDGDKLIRELPFSSLIFARQWFVVGELLQHLPPEDRITPIVGMLQGVVEKGGE
                     LRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
                     GYSYSNNNSPFYMGIPRLKFPADAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDL
                     GACPGGWTYQLVKRNMWVYSVDNGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVC
                     DMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINA
                     QIQARQLYHDREEVTVHVRRIWAAVGGRRDER"
     gene            complement(13295..13690)
                     /gene="ygdD"
                     /locus_tag="ACHKBL_003179"
     CDS             complement(13295..13690)
                     /gene="ygdD"
                     /locus_tag="ACHKBL_003179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HFO5820014.1"
                     /translation="MTSRFMLIFAAISGFIFVALGAFGAHVLSKTMGAVEMGWIQTGL
                     EYQAFHTLAILGLAVAMQRRISIWFYWSSVFLALGTVLFSGSLYCLALSHLRLWAFVT
                     PVGGVSFLAGWALMLVGAIRLKRKGVSHE"
     gene            complement(13709..14626)
                     /gene="gcvA"
                     /locus_tag="ACHKBL_003180"
     CDS             complement(13709..14626)
                     /gene="gcvA"
                     /locus_tag="ACHKBL_003180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     regulator GcvA"
                     /protein_id="HFO5820015.1"
                     /translation="MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSL
                     EDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLLP
                     SFAIHWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
                     YAEYLLPVCSPLLLTGEKPLKTPEDLAKHTLLHDASRRDWQTYTRQLGLNHINVQQGP
                     IFSHSAMVLQAAIHGQGVALANNVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQA
                     ELGKIAAFRQWILAKAAAEQEKFRFRYEQ"
BASE COUNT         3580 a         3849 c         3606 g         3621 t
ORIGIN      
        1 gaagaagtca gatgtcgttt aatgggcaaa tattgccctt aaattctctt ttacttttga
       61 tttacagagt aaagcgttgg gataatctat cttccaagta gattattgta tttgggatca
      121 agatcactga tagatacata acttgtgtgt atctttccgc cctcaaatta ttacggcggt
      181 aaatgattaa gccatcgcct atagacagat ttcattttta cggtcaggca ccttcccggg
      241 ctgaactggc taaaagctga attatttgca ttcctccagg agaaatagat ggaaacgact
      301 caaaccagca cgattgcgtc gaaagactct cgtagtgcct ggcgcaagac agacaccatg
      361 tggatgctgg gcctttacgg cacggcaatc ggcgcgggcg tgctgttcct gccaatcaac
      421 gccggtgttg gcggtatgat cccgctgatc atcatggcta tccttgcgtt cccgatgaca
      481 ttttttgctc accgcggtct gactcgcttc gtactgtctg gtaaaaaccc gggcgaagac
      541 atcaccgagg ttgtagaaga acactttggt attggcgcag gtaaactgat taccctgctc
      601 tacttcttcg ctatctaccc gattctgctg gtttatagcg tggcaatcac caatacagtt
      661 gaaagcttca tgtctcacca gctgggtatg acaccaccgc cgcgtgcgat tctgtcgctg
      721 atcctgatcg tgggtatgat gaccatcgtt cgcttcggtg agcagatgat cgttaaagcg
      781 atgagtattc tggtattccc gtttgttggc gtactgatgc tgctggctct gtacctgatc
      841 ccgcagtgga acggcgcagc actggaaacg ctgtctctgg acactgcatc tgcaaccgga
      901 aacggtcttt ggatgaccct gtggctggca attccggtaa tggtgttctc gttcaaccac
      961 tctccgatca tctcttcttt cgccgttgcg aagcgtgaag agtacggcga tatggcagaa
     1021 cagaaatgct cgaagatcct ggcattcgca cacatcatga tggtgctgac cgtaatgttc
     1081 ttcgtcttca gctgtgtact gagcctgact ccagcagacc tggctgcggc taaagagcag
     1141 aacatctcga ttctgtctta cctggctaac cactttaacg cgccgatcat cgcgtggatg
     1201 gctccgatta tcgcgattat cgctatcacc aaatccttcc tcggccacta cctgggcgca
     1261 cgtgaaggct tcaacggtat ggtgattaaa tctctgcgtg gtaaaggtaa gtctatcgaa
     1321 atcaacaagc tgaaccgtat cactgcgctg ttcatgctgg taacgacctg gattgttgcc
     1381 accctgaacc cgagcatcct gggtatgatt gaaaccctgg gcggcccaat catcgcgatg
     1441 atcctgttcc tgatgccgat gtacgcaatt cagaaagtac cggcaatgcg taagtacagc
     1501 ggtcacatca gcaacgtatt cgttgtcgtg atgggtctga ttgcaatctc cgcaatcttc
     1561 tactctctgt tcagctaagt cctttcgcgc cgctttcggg cggcgcttcc tccgttttaa
     1621 cgcgatgtat ttcctatgat tagcgtattc gatattttca aaatcggcat tggcccttcc
     1681 agttctcata ccgttggacc aatgaaagcg ggtaaacaat ttaccgacga tctgattgcc
     1741 cgtaacctgc ttaaagacgt gacccgcgtg gtggttgacg tgtacggctc gctctctctg
     1801 accggtaaag gccaccacac tgatatcgcc attattatgg gcctggcggg taacctgccg
     1861 gataccgtgg atatcgattc catccctggt tttattcagg atgtgaatac tcatggtcgc
     1921 ctgatgctgg caaacggtca gcatgaagtg gagttcccgg ttgatcagtg catgaacttc
     1981 catgccgaca acctttctct gcatgaaaac ggtatgcgca ttaccgcact ggcgggcgat
     2041 aaagtcgttt acagccagac ttactactct atcggcggtg gctttatcgt tgatgaagaa
     2101 cattttggcc agcaggatag cgcaccggtt gaagttcctt atccgtacag ttcagcagcc
     2161 gatctgcaaa aacattgtca ggaaaccggg ctgtcactct ctggcctgat gatgaaaaac
     2221 gagctggcgc tgcacagcaa agaagagctg gaacagcacc tggcgaacgt ctgggaagtc
     2281 atgcgtggcg gtattgagcg tggtatttct accgaaggcg tgttacctgg caaactgcgc
     2341 gttccacgcc gtgctgcggc actacgccgg atgctggtca gccaggataa aaccaccact
     2401 gacccgatgg cggttgttga ctggatcaac atgtttgcac tggcagtgaa cgaagagaat
     2461 gctgctggcg gtcgcgtggt gactgcgccg actaacggtg cgtgcgggat tatcccggct
     2521 gtgctggcgt actacgataa gtttatccgc gaagtgaacg ctaactcact ggctcgttat
     2581 ctgctggtag ccagcgccat tggttctctt tataagatga acgcgtcgat ttctggtgcc
     2641 gaagtgggtt gccagggtga agttggcgtg gcgtgctcta tggcggcagc tggtctggca
     2701 gaactgttag gcgcaagccc ggcgcaggtg tgcatcgcgg cggaaatcgc catggagcat
     2761 aacctcggtc tgacgtgtga cccggttgct ggacaggtgc aggtaccgtg catcgagcgt
     2821 aacgccattg cggcagtaaa agcggtgaac gccgcgcgta tggcgctgcg ccgtaccagc
     2881 gagccgcgcg tctgcctcga taaagttatc gagaccatgt acgaaacagg taaagatatg
     2941 aacgccaagt accgcgaaac ctctcgcggt ggcctggcaa tgaagatcgt tgcctgcgat
     3001 taatctctcc cggaggcctc gttttgcgag gcctcttccc gttttctcaa ccagccatag
     3061 cctgttccgg catcgaatgt tacccttatc gcctgatctt taagggggtt atcgtggctg
     3121 ttcatttgct tattgtcgat gcactgaatc ttattcgtcg cattcatgcc gttcagggtt
     3181 cgccctgtgt cgaaacctgc cagcacgcgc tcgatcagct cattatgcac agccagccaa
     3241 cccacgcggt cgccgttttt gatgatgaaa accgcagtag cggctggcgt catcagcgtt
     3301 taccagatta caaagcgggt cgaccgccaa tgccggaaga gttgcacgac gagatgcctg
     3361 cattacgcgc cgcctttgag caacgcggcg tcccgtgctg gtccgccagc ggcaacgaag
     3421 ccgatgactt agccgccacg ctggcggtca aagtgacaca ggccgggcat caggcaacga
     3481 ttgtttcgac agataaaggc tactgtcagt tactttcacc gacattacgt attcgtgatt
     3541 acttccagaa acgttggctg gatgcgccat ttatcgataa agaatttggc gttcaaccgc
     3601 agcagttgcc cgattactgg ggacttgcgg ggatcagcag ttcaaaggta ccgggtgttg
     3661 tggggatcgg accaaaaagc gccacgcagc tgctggtcga gtttaagagt ctggaaggga
     3721 tatatgagaa tctgaatgcg gttgccgaaa agtggcgcaa aaaattagaa acccataaag
     3781 agatggcgtt tctgtgccgc gatattgccc gcttacaaac cgatttgcat atcgacggca
     3841 atttacagca attgcggttg gtaaggtaac ggcgtgccgg atacggcgca aacgtcgcat
     3901 ccggcattat cacatcagcg catttaccag gcggtatggt aaagctctac aatatcctca
     3961 agcgttgctt cacgcgggtt gccaccggta caaacatcat ccagtgccgc ctgcgccagt
     4021 gtcggaatat cttccttacg tacaccaaca tcacgtaaat gtggcggaat accgacatca
     4081 cggttgagag caaacaccgc ttcaacagcg gcattacgcg cctcttccag gctcatacct
     4141 tccactttca cgcccataac gcgtgcgata tcgcggtact tctcaccggt aaagtcagcg
     4201 ttataacgca taacatgcgg taacaggatg gcgttcgcaa caccgtgtgg cgtgttgtaa
     4261 aacgcgccca gtggatgcgc cataccatgc accaacccta acccaacatt cgagaatccc
     4321 atacccgcaa catactgccc gagcgccatt tcttctccgg catccttatc accagcaacc
     4381 gatcctcgca gcgccccagc aatgatttca atcgctttaa tgtgcagcgc atcggttagc
     4441 gcccacgcgc cacgggtaat atacccctca atagcatgag tgagcgcatc gacacccgtc
     4501 gcagctttca gcgctggagg cataccatcc atcatgtcag cgtcaataaa cgccacctgc
     4561 gggatatcat gcggatcaac gcaaacaaac ttgcgccgtt tttcttcgtc agtgatcacg
     4621 tagttaatgg tcacttctgc cgcagtacct gctgtggtgg ggattgccag aatcggtaca
     4681 ctgggtttat tggtcgggga aagcccttcc aggctacgca catcggcaaa ctccgggttg
     4741 ttgctgataa tgccaatcgc tttacaagta tcctgtggag aaccgccacc aatagcgatc
     4801 aggtaatccg cgccgctatt ctggaataca ccgagccctt ctttgacgac agtaattgtt
     4861 gggttcggca ctacgccgtc gtaaatcgcc catgccagcc ctgcagcatc catcttatcg
     4921 gtcacttttg ccaccacgcc gcattgcacc agcgttttat cggtgacgat cagcgccttc
     4981 tgataaccac ggcgtttcac ctcatcggtt aaagacccaa cagccccccg accaaaccat
     5041 gccgtttcgt tcagaatcat tctgttagcc atcatccttc tccttgttgc tttacgaaat
     5101 tactcttcaa tccgtaaccc gtaggttttg aatttctcca gcactacggc aatctcttca
     5161 tcgctcagca ctggcaccgg gtccgtaatc gccagggtcg tcaggtaaag ttgtgccagc
     5221 acttcaactt catgcgccag ccataacgct ttctccagat tcgcctcaca agcgataagc
     5281 ccatgatgtt gtaacaaagt tgccttacga tttttgagag ccagcgcaac atgttcagaa
     5341 agttcgcgtg ttccaaaggt cgcataaggc gcgcaaggaa tagaattacc gccagccgcc
     5401 gcaatcatgt agtgaatcgc ggggatcggt cggttaagaa tggaaactgc cgtacaatga
     5461 acggcatgat tgtgaacaac cgcgttggca tccggtctgc tttgataggc cgccatatgg
     5521 aaacgccact cgcttgaggg gagttttcct tcctcatgtt tgccgttgcc atcaataaag
     5581 acaatatgcg actccgtcag tttttcatat ggaatgcctg taggcgtaat cagcatccca
     5641 tcctgataac gtacactgac gttacccgct gtcccctggt tcagtcccag gcgggtcatt
     5701 tccaggcaag tgtcaataat ctgacgagca agtttatttc gttccattag ctacctctct
     5761 ctgattcaaa acagggcaat aatgttgttc ctttcacact attgaattag ccgtttaatt
     5821 acccaccatc ttcttcctga ttaaccagaa agaaattcac aagcttatat tttgtgacct
     5881 ggttcaacta atcacagtaa ataactgcaa gttctctttt tataacccta ttaaaaatga
     5941 ccgctcttaa aaatatttat caaaacggtc atttttccat tccccctcgc cagaaatgcc
     6001 cgttttcggc acaaacaatt aatacggtca tcgggtttgt gttttttatg atttattttc
     6061 tgaaacgggc atgaaatttc gattattaaa gtgatggtag tcacataaag tcacctccca
     6121 actaataagt gtgaccgccg tcatattaca gagcgttttt tatttgaaaa tgaatccatg
     6181 agttcatttc tgacaggcaa atattcactg atatgaagcc cgaactcgct ggttttgcac
     6241 ttttgaaaac ataaccgatt acgtgcttaa gcttctgaac ctaagaggat gctatgggaa
     6301 acacatcaat acaaacgcag agttaccgtg cggtagataa agatgcaggg caaagcagaa
     6361 gttacattat tccattcgcg ctgctgtgct cactgttttt tctttgggcg gtagccaata
     6421 accttaacga cattttatta cctcaattcc agcaggcttt tacgctgaca aatttccagg
     6481 ctggcctgat ccaatcggcc ttttactttg gttatttcat tatcccaatc cctgctggga
     6541 tattgatgaa aaaactcagt tataaagcag ggattattac cggattattt ttatatgcct
     6601 tgggcgctgc attattctgg cccgctgcag aaataatgaa ctacacctta tttttagttg
     6661 gcctatttat tattgcagct ggattaggtt gtctggaaac tgccgcaaac ccttttgtta
     6721 cggtattagg gccggaaagc agtggtcact tccgcttaaa tcttgcgcaa acatttaact
     6781 cgtttggcgc aattatcgcg gttgtctttg gacaaagtct tattttgtct aacgtgccac
     6841 atcaatcgca agacgttctc gataaaatgt ctccagagca attgagtgcg tataaacaca
     6901 gcctggtatt atcggtacag acaccttata tgatcatcgt ggctatcgtg ttactggtcg
     6961 ccctgctgat catgctgacg aaattcccgg cattgcagag tgataatcac agtgacgcca
     7021 aacaaggatc gttctccgca tcgctttctc gtctggcgcg tattcgtcac tggcgctggg
     7081 cggtattagc gcaattctgc tatgtcggcg cgcaaacggc ctgctggagc tatttgattc
     7141 gctacgctgt agaagaaatt ccaggtatga ccgcaggctt tgccgctaac tatttaaccg
     7201 gaaccatggt gtgcttcttt attggtcgtt tcaccggtac ctggctcatc agtcgcttcg
     7261 caccacacaa agtcctggca gcctacgcat taatcgctat ggcactgtgc ctgatctcag
     7321 ccttcgctgg cggtcatgtg ggcttaatag ccctgacttt atgcagcgcc tttatgtcga
     7381 ttcagtaccc aacaatcttc tcgctgggca ttaagaatct cggccaggac accaaatacg
     7441 gttcgtcctt catcgttatg accatcattg gcggcggtat tgtcactccg gtcatgggtt
     7501 ttgtcagtga cgcggcgggc aacatcccca ctgctgaact gatccccgca ctctgcttcg
     7561 cggtcatctt tatctttgcc cgtttccgtt ctcaaacagc aactaactga acatattttc
     7621 cgaataaagt gaggaatctg taatgaaaaa aatcagctta ccgaaaattg gtatccgccc
     7681 ggttattgac ggtcgtcgca tgggtgttcg tgagtcgctt gaagaacaaa caatgaatat
     7741 ggcgaaagcc acggctgcac tgctgaccga gaaactgcgc catgcctgtg gagctgccgt
     7801 cgagtgtgtc atttccgata cctgtatcgc gggtatggct gaagccgctg cttgcgaaga
     7861 aaaattcagc agtcagaatg taggcctcac cattacggta accccttgct ggtgctatgg
     7921 cagtgaaacc atcgatatgg atccaacccg cccgaaagcc atttggggct ttaacggcac
     7981 tgaacgtccc ggcgctgttt acctggcagc ggctctggca gctcacagcc agaaaggcat
     8041 cccggcattc tccatttacg gtcatgacgt tcaggatgcc gatgacacat cgattcctgc
     8101 cgatgttgaa gaaaaactgc tgcgctttgc ccgcgccggt ttagcagtcg ccagcatgaa
     8161 aggtaaaagc tatctgtcgc tgggcggcgt ttcgatgggt atcgccggtt ccatcgttga
     8221 tcacaacttc tttgaatcct ggctgggaat gaaagtccag gcggtggata tgaccgaact
     8281 gcgtcgccgt atcgatcaga agatttacga cgaagccgaa ctggaaatgg cgctggcctg
     8341 ggctgataaa aacttccgct atggcgaaga tgaaaataac aaacagtatc aacgcaatgc
     8401 cgagcaaagc cgcgcagttc tgcgcgaaag tttactgatg gcgatgtgta tccgcgacat
     8461 gatgcaaggc aacagcaaac tggccgatat tggccgcgtg gaagaatcac ttggctacaa
     8521 cgccatcgct gcaggcttcc aggggcaacg tcactggacc gatcaatatc ccaatggcga
     8581 taccgctgaa gcgatcctca acagttcatt tgactggaat ggcgtgcgca aaccgtttgt
     8641 cgtggcgacc gaaaacgaca gtcttaacgg cgtggcaatg ttaatgggcc accagctcac
     8701 cggcaccgct cagatatttg ccgatgtgcg tacctactgg tcaccagaag caattgagcg
     8761 tgtaacgggg cataaactgg acgggctggc agaacacggc atcatccatt tgatcaactc
     8821 cggttctgct gcgctggacg gctcctgtaa acaacgcgac agcgaaggta aaccgacgat
     8881 gaagccgcac tgggaaatct ctcagcaaga ggctgacgct tgcctcgccg ctaccgaatg
     8941 gtgcccggcg atccacgaat acttccgtgg cggcggttac tcttcccgct tccttaccga
     9001 aggcggcgtc ccgttcacca tgactcgtgt caacatcatc aaaggcctgg gaccggtact
     9061 gcaaatcgcg gaaggctgga gcgtggaatt gccgaaggat gtgcatgaca tcctcaacaa
     9121 acgcaccaac tcaacctggc caaccacctg gtttgcaccg cgcctcaccg gtaaagggcc
     9181 gtttacggat gtgtactcgg taatggcgaa ctggggcgct aaccacggag ttctgaccat
     9241 cggccacgtt ggcgcagact ttatcactct tgcctccatg ctgcgtatcc cggtatgtat
     9301 gcacaacgtt gaagagacca aagtgtatcg tccttctgcc tgggccgcgc acggcatgga
     9361 tattgaaggc caggattacc gcgcttgcca gaactacggc ccgttgtaca agcgttaata
     9421 catgactccc tgatgtgatg cccggtcgct ccggctaccg ggcctgaaca agcaagagtg
     9481 gttagccgga taagcaatgt tatccggcta tattgcagga gcgattatga aacaagaagt
     9541 tatcctggta ctcgactgtg gcgcgaccaa tgtcagggcc atcgcggtta atcggcaggg
     9601 aaaaattgtt gcccgcgcct caacgcctaa tgccagcgat atcgcgatgg aaaacaacac
     9661 ctggcaccag tggtctttag acgccatttt gcaacgcttt gctgattgct gtcggcaaat
     9721 caatagtgaa ctgactgatt gccatatccg cggtatcgcc gtcaccacct ttggtgtgga
     9781 tggcgctctg gtagataagc aaggcaatct gctctatccg atcattagct ggaaatgtcc
     9841 gcgaacagcc gcggtaatgg acaatattga acggttgatc tccgcacagc agttgcaggc
     9901 tatttctgga gtcggagcct ttagtttcaa tacgttatat aagttggtgt ggttgaaaga
     9961 aaatcatcca caactgctgg aacgcgcgca cgcctggctc tttatttcgt cactgattaa
    10021 ccaccgttta accggcgaat tcactactga tattacgatg gccggaacca gccagatgct
    10081 ggatatccag caacgcgatt tcagtccgca aattttacaa gccaccggta ttccacgccg
    10141 actcttccct cgtctggtgg aagcgggtga acagattggt acgctacaga acagcgccgc
    10201 agcaatgctc ggcttacccg ttggcatacc ggtgatttcc gcaggtcacg atacccagtt
    10261 cgcccttttt ggcgctggtg ccgaacaaaa tgaacccgtg ctctcttccg gtacatggga
    10321 aattttaatg gtccgcagcg cccaggttga tacttcgctg ttaagtcagt acgccggttc
    10381 tacttgcgaa ctggatagcc aggcagggtt gtataaccca ggtatgcaat ggctggcatc
    10441 cggcgtgctg gaatgggtga gaaaactgtt ctggacagct gaaactccct ggcaaatatt
    10501 gattgaagaa gctcgcctga tcgcgcctgg cgcagatgga gtgaaaatgc agtgtgattt
    10561 attgtcgtgc cagaacgctg gctggcaagg cgtaacgctt aataccacgc gggggcattt
    10621 ctatcgcgcg gcgctggaag ggttaaccac gcaattacag cgcaatctac agatgctgga
    10681 aaaaatcggg cactttaaag cctctgaatt attgttagtc ggcggaggaa gtcgcaacac
    10741 attgtggaat cagattaaag ccaatatgct tgatattccg gtaaaagttc tcgacgatgc
    10801 cgaaacgacc gtcgcaggag ctgcgctgtt tggttggtat ggcgtagggg aatttaacag
    10861 tccggaagaa gcccgcgcgc agattcatta tcagtaccgt tatttctacc cgcaaactga
    10921 acctgaattt atagaggagg tgtgaaatgc tgaaaacaat ttcgccgtta atttctcccg
    10981 aactattgaa agtgctggca gagatggggc atggagatga aattattttt tccgatgctc
    11041 actttcccgc ccattcgatg ggaccgcagg tgatccgcgc tgatggcctg ttggtgagcg
    11101 acttgctcca ggcgattatc ccgttatttg agctggacag ttatgcacca ccgctggtga
    11161 tgatggcggc ggtagaaggt gacactctag atcctgaagt agaacgacgt tatcgtaatg
    11221 cgctttcact acaagccccc tgtcctgaca tcatccgcat caatcgtttt gcgttttatg
    11281 aacgggcaca aaaagccttt gcgatcgtta tcacaggcga acgagcgaag tacgggaata
    11341 ttcttttaaa aaaaggggta acaccgtaat ctcataccgg tacgcccgca tgacgcgggc
    11401 ggttatcgaa tgatggggtg aaaaatatga aagcggcacg ccagcaagcg atagtcgacc
    11461 tgctgctgaa ccataccagc ctgaccacgg aagctctctc tgaacagcta aaggtcagta
    11521 aagaaaccat tcgtcgcgat ctcaatgaat tacagacgca gggtaaaatt ctgcgcaatc
    11581 atgggcgcgc taaatatatc caccgtcaaa atcaagacag tggcgatccc tttcacatcc
    11641 ggctgaaaag ccattatgcg cataaagcag atatcgcgcg cgaggcgctc gcgtggattg
    11701 aagaagggat ggtaatagcc ttagacgcaa gttcaacctg ctggtatctg gcacgccagt
    11761 tgcctgacat caacatccag gtcttcacca atagccatcc gatttgccat gaactcggta
    11821 aacgcgaacg cattcaactg atcagttccg gcggcacact tgagcgcaaa tatggttgtt
    11881 acgtcaatcc ctcgctgatt tcccagctta aatcgctgga aatcgatctg tttatttttt
    11941 cttgtgaagg gatcgatagc agcggcgcac tgtgggactc caatgccatc aacgctgatt
    12001 acaaatcgat gctattaaaa cgtgccgcgc aatcgttgtt attgattgat aaaagtaaat
    12061 ttaatcgttc aggggaagcc cgcatcgggc atctggatga ggtcacgcac attatttctg
    12121 atgagcgcca ggttgcagct tctttggtaa cagcctgacg gaaaggatag caggccggaa
    12181 acgacgcccc ggccttgcct gttatcgctc gtcgcgacga ccacccaccg cagcccagat
    12241 acggcggacg tgcaccgtca cttcttcgcg atcgtgatac aactgccgcg cctgaatctg
    12301 agcatttatg ccatgttcat caagctgtgc ctgaatatac gccagattgt gtgacacttc
    12361 ttcgtagcgt tttttcatcg gcagtttgag gttgaaaatg gtttcacggc accagccatt
    12421 aaccagccac tgcgccatca atgccgcaac tttcgccggt ttttcaacca tatcgcatac
    12481 catccaggag atattgctgc gtgtcggacg gaatttgaaa ccgtcttccc gcagccacgt
    12541 cacctgtccg gtatccatca gactttgcgc catcgggccg ttgtcgacgg aataaaccca
    12601 catgttgcgc ttcaccagtt ggtaggtcca gccgcctggg caagcgccta aatccaccgc
    12661 ccacatcccg ttcgccaggc gttcatccca ctcatccgca ggaataaaca catgaaatgc
    12721 ctcttccagt ttgagcgtgg aacgactcgg cgcatctgcc ggaaatttca ggcgcggaat
    12781 gcccatatag aacggcgaat tattgttgct gtatgagtaa ccggtatagc agcagcctgg
    12841 tgcaataaag aatacatgca caaccggacg cttcggcgtt tcatagttcg ccagcacacc
    12901 cgcatcgcgc aaggcagcgc gtagcggcac ggtgaattta cggcagaatt tcagcaactc
    12961 tttgctttcg ttggtatcgg caacttcaac acgcagttca ccgcccttct ctactacacc
    13021 ctgtaacatg ccgacaatgg gggtaatacg atcttctggc ggcaaatgct gcaggagttc
    13081 ccccaccaca aaccactggc gggcaaaaat taatgaactg aacggcagct cacggattaa
    13141 cttatcgcca tcatcaggtt gataacattc ataaatgaca taacccgcat tctctttcac
    13201 gcgggcaaaa ccgaaaattt cccgctggcc ggctttatcg gtaatttctg cggcgcactc
    13261 tttttcaaag cccggacggc acagcaatac aaccttattc atgacttacg cccttgcgct
    13321 ttaaacggat agcaccaact aacattaacg cccagcccgc aaggaagctc acgccgccaa
    13381 ccggtgtgac aaacgcccac aaacgcagat gggacagcgc caggcaataa aggctgccgc
    13441 tgaacagcac cgtgcctaac gcgaggaaaa cgctactcca gtaaaaccag atactgatgc
    13501 gacgctgcat tgccaccgcc agacctaaga tcgccagcgt atgaaacgcc tggtattcga
    13561 ggccggtctg gatccagccc atctcaacgg cccccatggt tttacttaac acatgcgcgc
    13621 caaaagcgcc cagagctaca aaaatgaagc cgctaatggc ggcgaaaatc agcataaaac
    13681 ggctggtcat ggtcgtaccc tacgtaaatt attgttcata acgaaagcgg aatttttctt
    13741 gttcagcagc ggctttcgcc aggatccatt ggcgaaaggc ggctatttta cccagttctg
    13801 cctggctgtc atgacaaacc agataaaaag cgtttttact gaccagaaca tcattaaacg
    13861 ggcaaacaag acgtccggcc tcaatttcag attgcgccat cacgttattt gccagcgcca
    13921 ctccctgccc gtggatagcc gcttgcagca ccatggcgct atggctaaaa attggccctt
    13981 gctgaacgtt gatatgattt aaccccaact gtcgggtata tgtctgccag tcacgacgcg
    14041 acgcatcatg taataacgta tgtttagcca gatcttccgg ggtcttcaag ggtttttcgc
    14101 cagtcagcag tagcggcgaa cacaccggca ataaatattc ggcgtaaagt ttttccaccc
    14161 gtagccccgg ccagttgccc cgaccataaa atatcgccac atcaacatca tccgccagct
    14221 tatcttcctg acgatcaacc gcctggattc gaacgtcaat tcccggataa gctgaattaa
    14281 agctggaaag tcgcggaacc aaccaatgaa tggcgaaact ggggagtaaa ctgaccgtca
    14341 acgccccctt agcgctacgg gcctggagtt tacgcgtcgc ttcggttaat tgcgaaaata
    14401 tctctttgat atcgaggaaa tagctttgcc cttcctcggt cagcaggagt gaacgattac
    14461 ggcggcggaa cagttttagc cccagaaaat cctcaagaga cttgatttga tgacttactg
    14521 cggcctgggt cacaaaaagc tcttctgctg cgcgagtgaa acttaaatgg cgtgctgcgg
    14581 catcaaaaac tcgtaaggca tttagcggtg gtaatcgttt agacatggct tttaaacttt
    14641 gatgttaaat gaattt
//
LOCUS       DAZFLD010000125        14579 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.126, whole genome shotgun sequence.
ACCESSION   DAZFLD010000125 DAZFLD010000000
VERSION     DAZFLD010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14579)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14579)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14579
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.126"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            88..1080
                     /gene="asnA"
                     /locus_tag="ACHKBL_003181"
     CDS             88..1080
                     /gene="asnA"
                     /locus_tag="ACHKBL_003181"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004071 - aspartate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HFO5820016.1"
                     /translation="MKTAYIAKQRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDGT
                     QDNLSGCEKAVQVKVKALPDAQFEVVHSLAKWKRQTLGQHDFSAGEGLYTHMKALRPD
                     EDRLSPLHSVYVDQWDWERVMGDGERQFSTLKSTVEAIWAGIKATEAAVSEEFGLAPF
                     LPDQIHFVHSQELLSRYPDLDAKGRERAIAKDLGAVFLVGIGGKLSDGHRHDVRAPDY
                     DDWSTPSELGHAGLNGDILVWNPVLEDAFELSSMGIRVDADTLKHQLALTGDEDRLEL
                     EWHQALLRGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQCGVWPAAVRESVPSLL"
     gene            complement(1085..2536)
                     /gene="viaA"
                     /locus_tag="ACHKBL_003182"
     CDS             complement(1085..2536)
                     /gene="viaA"
                     /locus_tag="ACHKBL_003182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA stimulator ViaA"
                     /protein_id="HFO5820017.1"
                     /translation="MLTLDTLNVMLAVSEEGLIEEMIIALLASPQLAVFFEKFPRLKA
                     AITDDVPRWREALRSRLKDARVPPELTEEVMCYQQSQLLSTPQFIVQLPQILDLLHRL
                     NSPWAEQARQLVDANSTITSALHTLFLQRWRLSLIVQATTLNQQLLEEEREQLLSEVQ
                     ERMTLSGQLEPILADNNTAAGRLWDMSAGQLKRGDYQLIVKYGEFLNEQPELKRLAEQ
                     LGRSREAKSIPRNDAQMETFRTMVREPATVPEQVDGLQQSDDILRLLPPELATLGITE
                     LEYEFYRRLVEKQLLTYRLHGESWREKVIERPVVHKDYDEQPRGPFIVCVDTSGSMGG
                     FNEQCAKAFCMALMRIALAENRRCYIMLFSTEIVRYELSGPQGIEQAIRFLSQQFRGG
                     TDLASCFRAIMERLQSREWFDADAVVISDFIAQRLPDDVTSKVKELQRVHQHRFHAVA
                     MSAHGKPGIMRIFDHIWRFDTGMRSRLLRRWRR"
     gene            complement(2530..4026)
                     /gene="ravA"
                     /locus_tag="ACHKBL_003183"
     CDS             complement(2530..4026)
                     /gene="ravA"
                     /locus_tag="ACHKBL_003183"
                     /EC_number="3.6.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA"
                     /protein_id="HFO5820018.1"
                     /translation="MAHPHLLAERISRLSSSLEKGLYERSHAIRLCLLAALSGESVFL
                     LGPPGIAKSLIARRLKFAFQNARAFEYLMTRFSTPEEVFGPLSIQALKDEGRYERLTS
                     GYLPEAEIVFLDEIWKAGPAILNTLLTAINERQFRNGAHVEKIPMRLLVAASNELPEA
                     DSSLEALYDRMLIRLWLDKVQDKANFRSMLTSQQDENDNPVPASLQVTDEEYERWQKE
                     IGEITLPDHVFELIFMLRQQLDKLPDAPYVSDRRWKKAIRLLQASAFFSGRSAVAPVD
                     LILLKDCLWYDAQSLNLIQQQIDVLMTGHAWQQQGMLTRLGAIVQRHLQLQQQQSDKT
                     ALTVIRLGGIFSRRQQYQLPVNVTASTLTLLLQKPLKLHDMEVVHISFERNALEQWLS
                     KGGEIRGKLNGIGFAQKLNLEVDSAQHLVVRDVSLQGSTLALPGSSAEGLPGEIKQQL
                     EELESDWRKQHALFSEQQKCLFIPGDWLGRIEASLQDVGAQIRQAQQC"
     gene            4249..6117
                     /gene="kup"
                     /locus_tag="ACHKBL_003184"
     CDS             4249..6117
                     /gene="kup"
                     /locus_tag="ACHKBL_003184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity potassium transporter Kup"
                     /protein_id="HFO5820019.1"
                     /translation="MSTDNKQSLPAITLAAIGVVYGDIGTSPLYTLRECLSGQFGFGV
                     ERDAVFGFLSLIFWLLIFVVSIKYLTFVMRADNAGEGGILTLMSLAGRNTSARTTSML
                     VIMGLIGGSFFYGEVVITPAISVMSAIEGLEIVAPQLDTWIVPLSIIVLTLLFMIQKH
                     GTAMVGKLFAPIMLTWFLILAGLGLRSIIANPEVLHALNPMWAVHFFLEYKTVSFIAL
                     GAVVLSITGVEALYADMGHFGKFPIRLAWFTVVLPSLTLNYFGQGALLLKNPEAIKNP
                     FFLLAPDWALIPLLIIAALATVIASQAVISGVFSLTRQAVRLGYLSPMRIIHTSEMES
                     GQIYIPFVNWMLYVAVVIVIVSFEHSSNLAAAYGIAVTGTMVLTSILSTTVARQNWHW
                     NKYFVALILIAFLCVDIPLFTANLDKLLSGGWLPLSLGTVMFIVMTTWKSERFRLLRR
                     MHEHGNSLEAMIASLEKSPPVRVPGTAVYMSRAINVIPFALMHNLKHNKVLHERVILL
                     TLRTEDAPYVHNVRRVQIEQLSPTFWRVVASYGWRETPNVEEVFHRCGLEGLSCRMME
                     TSFFMSHESLILGKRPWYLRLRGKLYLLLQRNALRAPDQFEIPPNRVIELGTQVEI"
     gene            6284..6703
                     /gene="rbsD"
                     /locus_tag="ACHKBL_003185"
     CDS             6284..6703
                     /gene="rbsD"
                     /locus_tag="ACHKBL_003185"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HFO5820020.1"
                     /translation="MKKGTVLNSDISSVISRLGHTDTLVVCDAGLPIPKSTTRIDMAL
                     TQGVPSFMQVLGVVTNEMQVEAAIIAEEIKQHNPHLHETLLTHLEQLQKHQGNTIEIR
                     YTTHEQFKQQTAESQAVIRSGECSPYANIILCAGVTF"
     gene            6711..8216
                     /gene="rbsA"
                     /locus_tag="ACHKBL_003186"
     CDS             6711..8216
                     /gene="rbsA"
                     /locus_tag="ACHKBL_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter ATP-binding protein RbsA"
                     /protein_id="HFO5820021.1"
                     /translation="MEALLQLKGIDKAFPGVKALSGAALNVYPGRVMALVGENGAGKS
                     TMMKVLTGIYTRDAGTLLWLGKETTFTGPKSSQEAGIGIIHQELNLIPQLTIAENIFL
                     GREFVNRFGKIDWKTMYAEADKLLAKLNLRFKSDKLVGDLSIGDQQMVEIAKVLSFES
                     KVIIMDEPTDALTDTETESLFRVIRELKSQGRGIVYISHRMKEIFEICDDVTVFRDGQ
                     FIAEREVASLTEDSLIEMMVGRKLEDQYPHLDKAPGDIRLKVDNLCGPGVNDVSFTLR
                     KGEILGVSGLMGAGRTELMKVLYGALPRTSGYVTLDGHEVVTRSPQDGLANGIVYISE
                     DRKRDGLVLGMSVKENMSLTALRYFSRAGGSLKHADEQQAVSDFIRLFNVKTPSMEQA
                     IGLLSGGNQQKVAIARGLMTRPKVLILDEPTRGVDVGAKKEIYQLINQFKADGLSIIL
                     VSSEMPEVLGMSDRIIVMHEGHLSGEFTREQATQEVLMAAAVGKLNRVNQE"
     gene            8221..9186
                     /gene="rbsC"
                     /locus_tag="ACHKBL_003187"
     CDS             8221..9186
                     /gene="rbsC"
                     /locus_tag="ACHKBL_003187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002882531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HFO5820022.1"
                     /translation="MTTQTVSGRRYFTKAWLMEQKSLIALLVLIAIVSTLSPNFFTIN
                     NLFNILQQTSVNAIMAVGMTLVILTSGIDLSVGSLLALTGAVAASIVGIEVNALVAVA
                     AALALGAAIGAVTGVIVAKGRVQAFIATLVMMLLLRGVTMVYTNGSPVNTGFTENADL
                     FGWFGIGRPLGVPTPVWIMGIVFLAAWYMLHHTRLGRYIYALGGNEAATRLSGINVNK
                     IKIIVYSLCGLLASLAGIIEVARLSSAQPTAGTGYELDAIAAVVLGGTSLAGGKGRIV
                     GTLIGALILGFLNNGLNLLGVSSYYQMIVKAVVILLAVLVDNKKQ"
     gene            9211..10101
                     /gene="rbsB"
                     /locus_tag="ACHKBL_003188"
     CDS             9211..10101
                     /gene="rbsB"
                     /locus_tag="ACHKBL_003188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
                     /protein_id="HFO5820023.1"
                     /translation="MNMKKLATLVSAVALSATVSANAMAKDTIALVVSTLNNPFFVSL
                     KDGAQKEADKLGYNLVVLDSQNNPAKELANVQDLTVRGTKILLINPTDSDAVGNAVKM
                     ANQANIPVITLDRQATKGEVVSHIASDNVLGGKIAGDYIAKKAGEGAKVIELQGIAGT
                     SAARERGEGFQQAVAAHKFNVLASQPADFDRTKGLNVMQNLLTAHPDVQAVFAQNDEM
                     ALGALRALQTAGKSDVMVVGFDGTPDGEKAVNDGKLAATIAQLPDQIGAKGVETADKV
                     LKGEKVQAKYPVDLKLVVKQ"
     gene            10227..11156
                     /gene="rbsK"
                     /locus_tag="ACHKBL_003189"
     CDS             10227..11156
                     /gene="rbsK"
                     /locus_tag="ACHKBL_003189"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006014 - D-ribose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HFO5820024.1"
                     /translation="MQNAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKG
                     ANQAVAAGRSGANIAFIACTGDDSIGESVRQQLATDNIDISPVSVIKGESTGVALIFV
                     NGEGENVIGIHAGANAALSPALVEAQRERIANASALLMQLESPLESVMAAAKIAHQNK
                     TIVALNPAPARELPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKGIR
                     TVLITLGSRGVWASVNGEGQRVPGFRVQAVDTIAAGDTFNGALITALLEEKPLPEAIR
                     FAHAAAAIAVTRKGAQPSVPWREEIDAFLDRQR"
     gene            11160..12152
                     /gene="rbsR"
                     /locus_tag="ACHKBL_003190"
     CDS             11160..12152
                     /gene="rbsR"
                     /locus_tag="ACHKBL_003190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose operon transcriptional repressor RbsR"
                     /protein_id="HFO5820025.1"
                     /translation="MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELN
                     YAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQ
                     RMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
                     SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTG
                     DFEFNGGFDAMRQLLLHPLRPQAVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDI
                     ELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQLTPILMERGSA"
     gene            complement(12118..13545)
                     /gene="mdtD"
                     /locus_tag="ACHKBL_003191"
     CDS             complement(12118..13545)
                     /gene="mdtD"
                     /locus_tag="ACHKBL_003191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtD"
                     /protein_id="HFO5820026.1"
                     /translation="MSDKKKRSMAGLPWIAAMAFFMQALDATILNTALPAIAHSLNRS
                     PLAMQSAIISYTLTVAMLIPVSGWLADRFGTRRIFTLAVSLFTLGSLACALSNSLPQL
                     VVFRVIQGIGGAMMMPVARLALLRAYPRNELLPVLNFVAMPGLVGPILGPVLGGVLVT
                     WATWHWIFLINIPIGIAGLLYARKHMPNFTTARRRFDITGFLLFGLSLVLFSSGIELF
                     GEKIVASWIALTVIVTSIGLLLLYILHARRTPNPLISLDLFKTRTFSIGIVGNIATRL
                     GTGCVPFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQVLRRLGYRHTLVGI
                     TVIIGLMIAQFSLQSPAMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGN
                     SVLAVTQQLSISLGVAVSAAVLRVYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLL
                     KTTDGNNLIKRQRKSKPNRVPSESE"
     gene            complement(13568..14260)
                     /gene="yieP"
                     /locus_tag="ACHKBL_003192"
     CDS             complement(13568..14260)
                     /gene="yieP"
                     /locus_tag="ACHKBL_003192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HFO5820027.1"
                     /translation="MPLSAQQLAAQKNLSYVLAEKLAQRILKGEYEPGTILPGEIELG
                     EQFGVSRTAVREAVKTLTAKGMVLPRPRIGTRVMPQSNWNFLDQELLTWWMTEENFHQ
                     VIDHFLVMRICLEPQACLLAAMVGTAEQKAHLNTLMTEMVALKENFRRERWIEVDMAW
                     HEHIYEMSANPFLTSFASLFHSVYHTYFTSITSDTVIKLDLHQAIVDAIIQSDGDAAF
                     KACQALLRSPDK"
BASE COUNT         3525 a         3592 c         3845 g         3617 t
ORIGIN      
        1 tgagttaggc tttttattga atgattattg catgcgtgtc ggtttttgtt gcttaatcat
       61 aagcaacagg acgcaggagt ataaaaaatg aaaaccgctt acattgccaa acaacgtcaa
      121 attagcttcg tgaaatctca cttttcccgt caactggaag aacgtctggg gctgatcgaa
      181 gtccaggcgc cgattcttag ccgtgtgggg gatggcacgc aggataactt gtcgggctgt
      241 gaaaaagcgg tgcaggtaaa agtgaaagct ctgcctgatg cccagttcga agtggttcat
      301 tcactggcga agtggaaacg tcagacatta gggcaacacg acttcagcgc gggcgaaggg
      361 ctgtacacgc acatgaaagc ccttcgcccc gatgaagacc gtctttctcc gttgcactcg
      421 gtctatgttg accagtggga ctgggaacgc gtaatgggcg acggtgagcg tcaattctcg
      481 actctgaaaa gcacggtaga ggcgatctgg gcgggaatta aagcaaccga agctgcggtt
      541 agcgaagagt ttggcctggc accgttcctg ccggatcaga tccacttcgt acacagccag
      601 gagttactgt ctcgttatcc ggatctcgat gccaaagggc gtgagcgggc gattgcgaaa
      661 gatcttggcg cggtattcct cgtcgggatt ggcggcaagt tgagtgatgg tcatcgtcac
      721 gacgtgcgcg caccggatta tgatgactgg agcaccccgt cagagctggg ccatgcgggt
      781 ctgaacggcg atattctggt atggaacccg gtactggaag atgcgtttga gctttcttcc
      841 atggggatcc gcgtggatgc cgatacgttg aaacatcagc tggcgctgac cggtgatgaa
      901 gatcgcctgg agctggagtg gcatcaggcg ctgctgcgcg gtgaaatgcc gcagaccatc
      961 ggcggcggta tcgggcagtc tcgtttgacc atgctgctgc tgcaactgcc acatatcggc
     1021 caggttcagt gtggtgtatg gccggcagct gttcgcgaga gcgtcccttc tctgctgtaa
     1081 taatttatcg ccgccagcgt ctgagcaggc ggcttcgcat cccggtatca aagcgccaga
     1141 tatgatcgaa aatgcgcatg atgccgggtt tgccatgtgc agacatcgcc acggcatgaa
     1201 agcgatgctg atgtacgcgc tgtagctctt tcactttact cgtcacgtcg tcaggcaacc
     1261 gctgggcgat aaaatcagaa atcaccaccg catcggcatc aaaccattcc ctgctttgca
     1321 agcgttccat aatggcgcga aaacaactgg caagatcggt gccgccacga aactgctggc
     1381 ttaaaaaacg gattgcttgt tcgatgcctt gtgggcctga aagctcataa cggacgatct
     1441 cggtggaaaa taacataata tagcagcgcc ggttttcagc gagagcaatg cgcatcaagg
     1501 ccatgcagaa cgctttcgca cactgttcat taaagccgcc cattgagccg gaagtatcca
     1561 cacagacaat aaacggtccg cgcggctgtt cgtcgtaatc tttatgtacc accgggcgtt
     1621 cgatcacttt ttcacgccac gactcaccgt gcaggcgata ggtgagcaac tgtttttcca
     1681 ccagccgacg gtaaaactca tactccagtt ccgttatccc cagtgtcgcc agttctggcg
     1741 gcaggagacg taaaatatca tcgctttgtt gcagaccatc aacctgctca ggaaccgtcg
     1801 ccggttcgcg caccatggtg cggaaggttt ccatctgcgc atcgttgcgc ggtattgatt
     1861 tggcttcccg tgaacgcccc aactgttctg ccaggcgttt cagttccggc tgttcgttaa
     1921 gaaattcacc gtatttcaca atcaactgat agtcgccgcg tttaagctga ccggcgctca
     1981 tatcccacag acgaccagct gcggtattgt tatctgcgag aatcggttca agttgcccgc
     2041 tcagcgtcat gcgttcctga acttcactca acagttgttc gcgttcttct tctaatagct
     2101 gctgatttag cgtcgttgct tgcacgatca gacttaaacg ccagcgctgg agaaaaagcg
     2161 tgtgtaacgc tgaagtgatc gtactgttag catcaaccaa ctgtcgggct tgttctgccc
     2221 atggagaatt cagacgatgc agtaagtcca ggatctgtgg tagctgcaca ataaactgcg
     2281 gcgtggagag gagctggctt tgctgatagc acatcacctc ttcggtgagt tccggcggga
     2341 ctcgggcatc tttcagccga ctgcgcagcg cctcacgcca gcggggaaca tcatcagtga
     2401 ttgccgcctt cagtcgtggg aatttttcaa agaagaccgc cagctgcggt gaggccagca
     2461 gcgcgatgat catctcttcg atcaatccct cttcgctgac ggccagcatc acattaagcg
     2521 tatccagcgt tagcattgtt gtgcctggcg aatctgggcg ccgacatcct gtaggctggc
     2581 ttcaatgcga cctaaccagt cgccagggat aaacagacat ttttgctgtt cgctgaataa
     2641 agcgtgttgc ttgcgccagt cgctttcaag ctcttccagt tgttgtttta tttcacctgg
     2701 cagaccttca gccgatgaac cgggaagtgc cagcgtactg ccttgtaaac ttacatcgcg
     2761 tacaacaaga tgttgggcgc tatccacttc cagattcaat ttctgggcaa agccgatacc
     2821 gttcagtttg ccgcgaattt caccaccttt actcagccac tgttccagcg cgttacgctc
     2881 aaaggagata tgaaccactt ccatatcatg cagttttaac ggtttttgca gcagcagagt
     2941 cagagtggaa gcagtaacgt taacaggaag ttgatactgc tgacgacggc tgaaaatgcc
     3001 gcccagacga attaccgtta aggctgtttt atcgctttgt tgctgctgga gttgcaggtg
     3061 acgttgcaca atcgcgccca ggcgggtcaa cattccttgc tgttgccagg catgaccggt
     3121 catcaataca tcaatttgtt gttgtatcaa attcaggctt tgcgcgtcat accacaggca
     3181 atctttcagc aaaatgagat caaccggggc aacagcactg cgaccgctaa aaaaggcgct
     3241 ggcctgcaat aatcgaatcg cttttttcca gcgacgatcc gagacataag gcgcatccgg
     3301 taatttatcc agttgctggc gcagcataaa aatcagctca aatacatgat cgggcagcgt
     3361 aatttcacca atctctttct gccagcgttc atattcttca tctgtgacct gcaaggaggc
     3421 aggaaccgga ttgtcgtttt catcctgttg actggtcagc atggagcgga aattcgcttt
     3481 atcctgcact ttatctaacc acagacgaat cagcatgcgg tcatataacg cttccagact
     3541 gctgtctgct tccggcagct cgttggaggc cgccaccagc aggcgcatcg ggattttttc
     3601 tacgtgtgca ccgttgcgga actggcgctc gttaatggcg gtgagcaggg tattaagaat
     3661 tgccggaccc gctttccaga tctcatccag aaagacgatt tctgcttccg gcaggtaacc
     3721 gctggttaaa cgttcatagc gcccttcatc ttttagcgcc tgaatagaaa ggggaccaaa
     3781 aacctcttcc ggcgtggaga agcgagtcat cagatattca aacgcgcggg cattctgaaa
     3841 ggcgaatttt aagcgccggg cgatcaaact tttggcaata cctggcgggc caaggaggaa
     3901 cacactttca ccacttaatg ccgctaataa acacaagcgg atggcgtggc tacgttcata
     3961 aagccccttt tccaacgaac tgctcaggcg ggaaattctt tccgctaata aatgagggtg
     4021 agccataatg aagtggcgtc ctttcgtcaa aagttctgcg taaattgcga gtatagacgt
     4081 ttcttgctgg tggctaaaat agtctcaaag ggggggtatt tttctttgag ccaggttaat
     4141 gtgcccgcat ttaggagtac gattttgccg ttaatcgtgc atactgtgcg cttttttgtg
     4201 ggccaaggga ctaagcacac atttcatatt tcaacgaaag actagtctat gagcactgat
     4261 aataagcaat cattgcccgc gattaccctc gcggcgattg gagttgtcta cggcgatatt
     4321 ggtaccagcc cgttatatac acttcgtgaa tgtttgtccg gccagtttgg ttttggcgtt
     4381 gagcgcgatg ccgtgtttgg ctttttatcg ctgatcttct ggctgctaat ctttgtggtt
     4441 tctattaaat atctcacctt cgtgatgcgg gcagataacg ccggtgaagg ggggatcctg
     4501 acgttgatgt cgcttgccgg gcgtaatacg tcggcgcgaa ccacatcaat gctggtgatt
     4561 atggggctaa tcggcggcag ctttttctat ggtgaagtcg tcataacacc cgctatttcg
     4621 gtgatgtccg ccattgaagg tctggaaatc gtcgccccgc agctggatac ctggatagtt
     4681 cccctctcaa ttatcgttct cactttatta tttatgattc aaaagcatgg cacggctatg
     4741 gtcggtaagc tgtttgcgcc gatcatgctg acctggtttt tgattctggc aggactgggg
     4801 ttacgtagca ttattgctaa cccggaagtg ctgcatgcgc tgaatccgat gtgggcggtg
     4861 catttcttcc ttgaatacaa aacggtttct tttattgcat taggggcagt ggtgctgtcg
     4921 attacggggg tcgaggcgct gtatgctgat atggggcact ttggtaagtt ccctattcgc
     4981 ctggcgtggt tcaccgtcgt attaccttcc ttgaccctta attacttcgg tcagggagcg
     5041 ttgttgttaa agaacccgga agcgattaag aacccgttct tcctgttggc accggactgg
     5101 gcgctgatcc cgctgctgat catcgccgca ctggcgacgg taattgcctc gcaggctgtt
     5161 atctctggcg tcttctcatt gacgcgtcag gcggtacgtc tgggatattt gtcgccgatg
     5221 cgcattattc acacctccga aatggagtca gggcaaatct atattccctt tgtgaactgg
     5281 atgctctatg tcgcggtcgt gattgtgatt gtcagctttg agcactccag caacctggcg
     5341 gcggcgtacg ggattgcggt gaccggaacc atggtgctga cgtctattct ctcgactacc
     5401 gtggcacgtc agaactggca ctggaataag tattttgttg cgctgatcct gattgctttc
     5461 ctttgtgtcg atattccatt gttcaccgct aacctcgata aactgctctc cggcggctgg
     5521 ttgccattga gcctcggtac ggtgatgttt atcgtgatga ccacctggaa gagcgaacgt
     5581 ttccgtttgc tgcggcggat gcatgaacat ggtaactctc tggaggcgat gattgcttcg
     5641 ctggagaaat caccgcccgt tcgcgtgccc gggaccgcgg tgtatatgtc acgtgcaatc
     5701 aacgtcattc cctttgcgct gatgcataac cttaaacata acaaggtatt gcatgagcgg
     5761 gtgattctgt taactctgcg caccgaagac gcgccatatg tccataacgt ccgtcgggta
     5821 cagattgaac aactgtcgcc cactttctgg cgcgtggtgg caagttatgg ttggcgagaa
     5881 acgccaaacg tggaagaagt tttccaccgc tgcggtctgg aaggattaag ttgccggatg
     5941 atggaaacct ccttctttat gtcgcatgag tcgttgatcc tcggcaaacg cccgtggtat
     6001 ttgcgtctgc gcggcaagct gtacttgctg ctgcaacgta atgcgctgcg tgcgccggat
     6061 caatttgaaa tcccgccaaa cagggttatc gaactgggta ctcaggtcga aatctaacgc
     6121 cagacgcctc ctttctcctt aagggggcgt ttctgttttc atggttaatt accatgtaaa
     6181 acgtttcgag gttgatcaca tttccgtaac gtcacgatgg ttttcccaac tcagtcagga
     6241 ttaaactgcg ggtcagcgaa acgtttcgct gatggagaaa aaaatgaaaa aaggcaccgt
     6301 tcttaattct gatatttcat cggtgatctc ccgtctggga cataccgata cgctggtggt
     6361 gtgtgatgct ggtttaccca tccccaaaag tacaacgcgt atcgatatgg cattaaccca
     6421 gggcgtacct tcttttatgc aggtgctggg cgtcgtcaca aatgaaatgc aggtcgaggc
     6481 agccattatc gcggaagaga tcaaacaaca taatccgcat ctccacgaaa cgttgctcac
     6541 tcaccttgag cagctgcaaa aacaccaggg aaataccatt gaaattcgtt acaccacgca
     6601 tgaacaattc aaacaacaaa ccgcagaaag tcaggcggta attcgcagcg gagaatgttc
     6661 tccgtatgcg aatatcattc tctgtgctgg cgtgacgttc tgaggccgtc atggaagcat
     6721 tacttcagct taaaggcatc gataaagcct tcccgggcgt aaaagccctc tcgggcgcag
     6781 cgttaaatgt ctatcctggc cgcgtgatgg cgctggtggg cgaaaacggt gcgggtaaat
     6841 ccaccatgat gaaagtgctt acgggtatct atactcgcga tgccggtacg cttttatggc
     6901 tggggaaaga aacgacgttt accgggccga aatcttccca ggaagccggg attgggatta
     6961 tccatcagga actgaacctg atcccgcagt tgaccattgc cgaaaacatt ttcctcggtc
     7021 gtgagtttgt taatcgcttt ggcaaaattg actggaaaac catgtatgcc gaagcggata
     7081 aattgctggc taaacttaac ctgcgcttta aaagcgacaa gctggtgggc gatctttcca
     7141 tcggtgacca gcaaatggtt gaaattgcca aagtgctgag ctttgagtcg aaagtcatca
     7201 ttatggatga accgaccgat gcgctgaccg ataccgaaac cgaatccttg ttccgcgtca
     7261 tccgcgagct gaaatcgcaa ggccgcggta ttgtctatat ctcccaccgc atgaaagaaa
     7321 tcttcgagat ttgcgatgac gttaccgttt ttcgtgatgg gcaatttatt gctgagcgtg
     7381 aagtagcatc actgaccgaa gattcgctga ttgagatgat ggtgggtcgc aagctggaag
     7441 atcaatatcc gcacctggac aaagcgccgg gagatatccg cctgaaagtc gataatctct
     7501 gcggacctgg cgttaacgat gtctctttta ctttacgcaa aggcgaaatt cttggcgtct
     7561 ctggtttgat gggcgcaggt cgtaccgaac tgatgaaagt gctctacggc gcactgccgc
     7621 gcaccagcgg ttacgtcacc ctggatgggc atgaagtcgt tacccgttca ccgcaggatg
     7681 gcctggcaaa cggcattgtg tatatctccg aagaccgtaa acgtgacggt ttagtgctgg
     7741 gcatgtcagt aaaagagaac atgtcgctga cagcgctgcg ctacttcagc cgcgctggcg
     7801 gcagtttgaa gcatgccgat gaacagcagg cagtaagtga tttcattcgt ctgtttaatg
     7861 tgaaaacgcc gtcgatggaa caggcaattg gtctgctctc cggtggcaat cagcaaaaag
     7921 tggcgattgc ccgtggtctg atgacacgcc ccaaagtgtt gatcctcgat gagcctaccc
     7981 gtggcgtaga tgtcggcgcg aaaaaagaga tctatcaact gattaaccag ttcaaagccg
     8041 atggcttgag catcattctg gtgtcatcgg agatgccaga agtattaggc atgagcgatc
     8101 gcatcatcgt catgcatgaa gggcatctca gcggggaatt tactcgtgag caggccaccc
     8161 aggaagtgtt aatggctgcc gctgtgggca agcttaatcg cgtgaatcag gagtaaaaaa
     8221 atgacaaccc agactgtctc tggtcgccgt tatttcacga aagcgtggct gatggagcag
     8281 aaatcgctta tcgctctgct ggtgctgatc gcgattgtct cgacgttaag cccgaacttt
     8341 ttcaccatca ataacttatt caatattctc cagcaaacct cggtgaacgc cattatggca
     8401 gtcgggatga cgctggtgat cctgacgtcg ggcatcgact tgtcggtagg ttctctattg
     8461 gcgctgaccg gcgcagttgc tgcatctatc gttggcattg aagtcaatgc gctggtggct
     8521 gtcgctgccg ctctcgcgtt aggtgccgca attggtgcgg taaccggggt gattgtagcg
     8581 aaaggtcgcg tccaggcctt tatcgctacg ctggttatga tgcttttact gcgcggcgtg
     8641 accatggttt ataccaacgg tagcccagtg aatactggct ttactgagaa cgccgatctg
     8701 tttggctggt ttggtattgg tcgtccgctg ggtgtaccga cgccagtctg gatcatggga
     8761 attgtcttcc tcgcggcctg gtacatgctg catcacacgc gtctggggcg ttacatctac
     8821 gcgctgggcg gcaacgaagc ggcaacgcgc ctttctggta tcaacgtcaa taaaatcaaa
     8881 atcatcgtct attctctttg tggtctgctg gcatcgctgg ccgggatcat tgaagtggcg
     8941 cgtctctcct ccgcacaacc tacggcgggg actggctatg agctggatgc cattgctgcg
     9001 gtggttctgg gcggtacgag tctggcgggc ggaaaaggtc gcattgttgg gacgttgatc
     9061 ggcgcattaa ttcttggctt ccttaataat ggattgaatt tgttaggtgt ttcctcctat
     9121 taccagatga tcgtcaaagc ggtggtgatt ttgctggcgg tgctggtaga caacaaaaag
     9181 cagtaataac gactacagga catcttgaat atgaacatga aaaaactggc taccctggtt
     9241 tccgctgttg cgctaagcgc caccgtcagt gcgaatgcga tggcaaaaga caccatcgcg
     9301 ctggtggtct ccacgcttaa caacccgttc tttgtatcgc tgaaagatgg cgcgcagaaa
     9361 gaggcggata aacttggcta taacctggtg gtgctggact cccagaataa cccggcgaaa
     9421 gagctggcga acgtgcagga cttaaccgtt cgcggcacca aaattctgct gattaacccg
     9481 accgactccg acgcagtggg taatgctgtg aagatggcta accaggcgaa catcccggtt
     9541 atcactcttg accgccaggc aacgaaaggt gaagtggtga gccacattgc ttctgataac
     9601 gtactgggcg gcaaaatcgc cggtgattac atcgcgaaga aagcgggtga aggtgccaaa
     9661 gttatcgagc tgcaaggcat tgccggtaca tccgcagccc gtgaacgtgg cgaaggcttc
     9721 cagcaggctg ttgctgctca caagtttaat gttcttgcca gccagccagc agattttgat
     9781 cgcactaaag gtttgaacgt aatgcagaac ctgttgaccg cacatccgga tgttcaggct
     9841 gtattcgcgc agaatgatga aatggcgctg ggcgcgctgc gcgcactgca aactgccggt
     9901 aaatcggatg tgatggtcgt cggatttgac ggtacaccgg atggcgaaaa agcggtgaat
     9961 gatggcaaac tagcagcgac tatcgctcag ctacccgatc agattggcgc gaaaggcgtc
    10021 gaaaccgccg ataaagtact gaaaggtgag aaagttcagg ctaagtatcc ggttgatctg
    10081 aaactggttg ttaagcagta gttttaatca ggttgtatga cctgatggtg acataaatac
    10141 ggcatcgaca gatgaacgtg taatataaag aaaagcaggg cacgcgccac cctaacacgg
    10201 tggcgcattt tatggacatc ccgaatatgc aaaacgcagg cagcctcgtt gttcttggca
    10261 gcattaatgc tgaccacatt cttaatcttc aatcttttcc taccccaggc gaaaccgtaa
    10321 ccggtaacca ctatcaggtt gcatttggcg gcaaaggcgc taatcaggct gtggctgctg
    10381 ggcgtagcgg tgcgaatatc gcgtttattg cctgcactgg cgatgacagc attggtgaga
    10441 gcgttcgcca gcagctcgcc actgataaca tcgatatttc tccggtcagc gtgatcaaag
    10501 gcgaatcaac aggtgtggcg ctgatttttg ttaatggcga aggtgagaat gtcatcggta
    10561 ttcatgccgg cgctaatgct gccctttccc cggcactggt ggaagcgcaa cgtgagcgta
    10621 ttgccaacgc gtcagcatta ttaatgcagc tggaatcacc actcgaaagt gtgatggcag
    10681 cggcgaaaat cgcccatcaa aataagacta tcgttgcgct taacccggct ccggctcgcg
    10741 aacttcctga cgaactgctg gcactggtgg acattattac gccaaacgaa acggaagcag
    10801 aaaagctcac cggtattcgt gttgaaaatg atgaagatgc agcgaaggcg gcgcaggtac
    10861 tgcatgaaaa aggtatccgt actgtactga ttactttagg aagtcgtggt gtatgggcta
    10921 gcgtgaatgg tgaaggtcag cgcgttcctg gattccgggt gcaggctgtc gataccattg
    10981 ctgccggaga tacctttaac ggcgcgttaa tcacggcgtt gctggaagaa aaaccattgc
    11041 cagaggcgat tcggtttgct catgctgccg ctgcgattgc cgtaacacgt aaaggcgcac
    11101 aaccttccgt accgtggcgt gaagagatcg acgcattttt agacaggcag aggtgacgct
    11161 tggctacaat gaaagatgtt gcccgcctgg cgggcgtttc tacctcaaca gtttctcacg
    11221 ttatcaataa agatcgcttc gtcagtgaag cgattaccgc caaagttgaa gcggcgatta
    11281 aagaactcaa ttacgcgcca tcagccctgg cgcgtagcct caaactcaat caaacacata
    11341 ccattggcat gttgatcact gccagtacca atcctttcta ttctgaactg gtgcgtggcg
    11401 ttgaacgcag ttgcttcgaa cgcggttata gtcttgtcct ttgcaatacc gaaggcgatg
    11461 aacagcggat gaatcgcaat ctggaaacgc tgatgcaaaa acgcgttgat ggcttgctgt
    11521 tactgtgcac cgaaacgcat caaccttcgc gtgaaatcat gcaacgttat ccgacagtgc
    11581 ctactgtgat gatggactgg gctccgttcg atggcgacag cgatcttatt caggataact
    11641 cgttgctggg cggagactta gcaacgcaat atctgatcga taaaggtcat acccgtatcg
    11701 cctgtattac cggcccgctg gataaaactc cggcacgcct gcggttggaa ggttatcggg
    11761 cggcgatgaa acgtgcgggt ctcaacattc ctgatggcta tgaagtcact ggtgattttg
    11821 aatttaacgg cgggtttgac gctatgcgcc aactgttatt acatccgctg cgtcctcagg
    11881 ccgtctttac cggaaatgac gctatggctg ttggcgttta ccaggcgctg tatcaggcag
    11941 agttacaagt tccgcaggat attgcggtga ttggctatga cgatatcgaa ctggcaagct
    12001 ttatgacgcc accattaacc actatccacc aaccgaaaga tgaactaggg gagctggcga
    12061 ttgatgtact catccatcgg ataacccaac cgacccttca gcaacaacga ttacaactta
    12121 ctccgattct gatggaacgc ggttcggctt agatttacgc tgtcttttga tcaaattatt
    12181 accatcggtt gttttcagaa gcatgaacat tgctgctgaa gcaacagtaa taatgcccat
    12241 cgtgataaac gtatagtgga attgttcgac agtcgttgtg ccttccatcc cttcataaac
    12301 gcgaaggacg gccgcactta cagcaacgcc taaactgata gacagttgct gcgtgaccgc
    12361 cagaacactg ttaccgctgc tggcgttgtc atcggtcaga tcggcaagtg tgatggtatt
    12421 catcgcggta aattgcgtcg acatagccat ccctaatata aacaacggca agatcagcat
    12481 ccatatcgcc attgccggtg attgcaaaga gaactgagcg atcattagcc caataatcac
    12541 cgtgatcccc actaatgtat ggcgatagcc cagacgacgt aagacttggg taaccatcga
    12601 ttttgcaata atggaaccta acgctgtcgg tgccatcata cagccggcaa taaacgcctg
    12661 ataaccaaat cctacctgta acatcaatgg cataaggaac ggtacacaac cggtccccag
    12721 acgggttgca atattgccta cgataccgat cgagaaagtg cgggttttaa ataaatctaa
    12781 tgaaattaat gggtttggcg tgcgtcgtgc atggagaata tagagaagca gtaacccgat
    12841 gctggtgaca attaccgtca aggcaatcca actggcgaca atcttttccc cgaatagctc
    12901 tattccgctt gagaagagaa caaggctaag gccaaacagc aaaaagccag tgatatcgaa
    12961 tctgcgtcgt gcggtggtga aattgggcat atgtttgcgc gcgtaaagaa ggcccgcaat
    13021 acctatgggg atattgatta aaaatatcca gtgccaggtt gcccaggtga ccagcacgcc
    13081 gccaagaaca gggcctaaaa ttggccccac cagacccggc atggcgacaa aattcaatac
    13141 aggaagaagc tcattacgag gataagcgcg cagtaaggcc agccgagcaa caggcatcat
    13201 cattgcgccg cctatcccct gaataacccg gaagacaacc agctgtggta gcgagttaga
    13261 aagtgcgcag gccagagaac ccaacgtgaa cagactcacg gcaagagtaa atatgcgacg
    13321 cgtaccgaag cgatcggcta gccatccgct taccggaata agcatcgcca ccgtcagcgt
    13381 ataactgatg atggctgatt gcatcgcgag aggagaacga ttaaggctat gagcgattgc
    13441 gggtaaggcg gtattcagaa tagtggcatc aagtgcctgc ataaagaagg ccatcgccgc
    13501 gatccacggc aaacccgcca tactgcgctt ctttttatcg ctcattcaat gtcctgttat
    13561 cggggtgtca cttatcaggt gagcgtagca gcgcctgaca agctttaaat gccgcgtcgc
    13621 catcgctttg gataatcgcg tcaacaatcg cctggtgcag atccagcttt atcactgtgt
    13681 cgctggtaat tgacgtgaag taagtgtgat aaaccgaatg gaagagcgag gcgaatgagg
    13741 tcaaaaacgg attggcgctc atttcataga tatgctcatg ccaggccata tcgacctcaa
    13801 tccagcgttc acggcgaaag ttctctttta atgccaccat ttcggtcatt aacgtattga
    13861 gatgcgcctt ctgttccgcg gtgccaacca ttgctgccag taggcaagct tgcggctcca
    13921 gacagatacg cataaccaga aagtgatcga tgacctgatg aaagttctct tctgtcatcc
    13981 accaggtaag caattcctga tcaagaaaat tccagtttga ttgtggcatg acccgggtac
    14041 caattcgcgg tcgcggtaaa accatccctt ttgccgttaa cgttttgacc gcttcgcgta
    14101 ccgctgtacg actcactcca aattgctcgc ccagctcaat ctcaccaggc aaaatggtgc
    14161 cgggttcata ttcacctttt aagatgcgtt gcgccagctt ctcagccaga acatacgaaa
    14221 ggtttttctg tgcagctaac tgttgtgcgc ttaaaggcat tactaatctt cctttttctt
    14281 tttattcctc cttagtatgc caccaggaag tgtgattacg gttgcaaaaa cggcaaattg
    14341 cttgttttat ggcacattaa cggggctttt gctgaaaaaa tgcgcggtca gaaaattatt
    14401 ttaaatttcc tcttgtcagg ccggaataac tccctataat gcgccaccac tgacacggaa
    14461 caacggcaaa cacgccgccg ggttagcggg gttctcctga gaattccggc agagaaagca
    14521 aaaacaaatg cttgactctg tagcgggaaa gcgtattatg cacaccccgc gccgctgag
//
LOCUS       DAZFLD010000126        14486 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.127, whole genome shotgun sequence.
ACCESSION   DAZFLD010000126 DAZFLD010000000
VERSION     DAZFLD010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14486
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.127"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            64..702
                     /gene="ompW"
                     /locus_tag="ACHKBL_003193"
     CDS             64..702
                     /gene="ompW"
                     /locus_tag="ACHKBL_003193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
                     /protein_id="HFO5820028.1"
                     /translation="MKKLTVAALAVTTLLSGSAFAHEAGEFFMRAGSATVRPTEGAGG
                     TLGSLGGFSVTNNTQLGLTFTYMATDNIGVELLAATPFRHKIGTRATGDIATVHHLPP
                     TLMAQWYFGDASSKFRPYVGAGINYTTFFDNGFNDHGKEAGLSDLSLKDSWGAAGQVG
                     VDYLINRDWLVNMSVWYMDIDTTANYKLGGAQQHDSVRLDPWVFMFSAGYRF"
     gene            complement(761..1267)
                     /gene="yciE"
                     /locus_tag="ACHKBL_003194"
     CDS             complement(761..1267)
                     /gene="yciE"
                     /locus_tag="ACHKBL_003194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFO5820029.1"
                     /translation="MNRIEHYHDWLRDAHAMEKQAESMLESMASRIDNYPELRARIEQ
                     HLSETKNQIVQLETILDRNDISRSVIKDSMSKMAALGQSIGGIFPSDEIVKGSISGYV
                     FEQFEIACYTSLLAAAKNAGDTASIPIIEAILNDEKHMADWLIQHIPQTTEKFLIRSE
                     TDGVEAKK"
     gene            complement(1313..1813)
                     /gene="yciF"
                     /locus_tag="ACHKBL_003195"
     CDS             complement(1313..1813)
                     /gene="yciF"
                     /locus_tag="ACHKBL_003195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFO5820030.1"
                     /translation="MNMKTIEDVFIHLLSDTYSAEKQLTRALAKLARATSNEKLSQAF
                     HAHLEETYGQIERIDQVVESESNLKIKRMKCVAMEGLIEEANEVIESTEKNEVRDAAL
                     IAAAQKVEHYEIASYGTLATLAEQLGYRKAAKLLKETLEEEKATDIKLTDLALNNVNK
                     KAENKA"
     gene            complement(1899..2078)
                     /gene="yciG"
                     /locus_tag="ACHKBL_003196"
     CDS             complement(1899..2078)
                     /gene="yciG"
                     /locus_tag="ACHKBL_003196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HFO5820031.1"
                     /translation="MAEHRGGSGNFAEDREKASDAGRKGGQHSGGNFKNDPQRASEAG
                     KKGGQQSGGNKSGKS"
     gene            complement(2459..3265)
                     /gene="trpA"
                     /locus_tag="ACHKBL_003197"
     CDS             complement(2459..3265)
                     /gene="trpA"
                     /locus_tag="ACHKBL_003197"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HFO5820032.1"
                     /translation="MERYESLFAQLKERKEGAFVPFVTLGDPGIEQSLKIIDTLIEAG
                     ADALELGIPFSDPLADGPTIQNATLRAFAAGVTPAQCFEMLALIRQKHPTIPIGLLMY
                     ANLVFNKGIDEFYAQCEKVGVDSVLVADVPVEESAPFRQAALRHNLAPIFICPPNADD
                     DLLRQIASYGRGYTYLLSRAGVTGAENRAALPLNHLVTKLKEYNAAPPLQGFGISAPD
                     QVKAAIDAGAAGAISGSAIVKIIEQHINEPEKMLAALKAFVQPMKAATRS"
     gene            complement(3265..4458)
                     /gene="trpB"
                     /locus_tag="ACHKBL_003198"
     CDS             complement(3265..4458)
                     /gene="trpB"
                     /locus_tag="ACHKBL_003198"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HFO5820033.1"
                     /translation="MTTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQ
                     FNDLLKNYAGRPTALTKCQNITAGTNTTLYLKREDLLHGGAHKTNQVLGQALLAKRMG
                     KTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVH
                     SGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVREFQRMIGEETKAQILE
                     REGRLPDAVIACVGGGSNAIGMFADFINETNVGLIGVEPGGHGIETGEHGAPLKHGRV
                     GIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALE
                     AFKTLCLHEGIIPALESSHALAHALKMMRENPEKEQLLVVNLSGRGDKDIFTVHDILK
                     ARGEI"
     gene            complement(4470..5831)
                     /gene="trpCF"
                     /locus_tag="ACHKBL_003199"
     CDS             complement(4470..5831)
                     /gene="trpCF"
                     /locus_tag="ACHKBL_003199"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HFO5820034.1"
                     /translation="MMQTVLAKIVADKAIWVEARKQQQPLASFQNEVQPSTRHFYDAL
                     QGARTAFILECKKASPSKGVIRDDFDPAHIATVYRNYASAISVLTDEKYFQGSFDFLS
                     IVSQIAPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLDDEQYRHLAAVAHSLEMG
                     VLTEVSNEEELERAIALGAKVVGINNRDLRDLSIDLNRTRELAPKLGHNVTVISESGI
                     NTYAQVRELSHFANGFLIGSALMAHDDLNAAVRRVLLGENKVCGLTRGQDAKAAYDAG
                     AIYGGLIFVATSPRCVNVEQAQEVMAAAPLQYVGVFRNHDIADVVDKAKVLSLAAVQL
                     HGNEDQLYIDTLREALPAHVAIWKALSVGETLPARELQHVDKYVLDNGQGGSGQRFDW
                     SLLNGQSLGNVLLAGGLGADNCVEAAQTGCAGLDFNSAVESQPGIKDARLLASVFQTL
                     RAY"
     gene            complement(5832..7427)
                     /gene="trpD"
                     /locus_tag="ACHKBL_003200"
     CDS             complement(5832..7427)
                     /gene="trpD"
                     /locus_tag="ACHKBL_003200"
                     /EC_number="2.4.2.18"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase glutamate
                     amidotransferase component TrpG/anthranilate
                     phosphoribosyltransferase TrpD"
                     /protein_id="HFO5820035.1"
                     /translation="MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHVPAQTLIERL
                     ATMSNPVLMLSPGPGVPSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAG
                     EILHGKASSIEHDSQAMFAGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHD
                     ADRVCGFQFHPESILTTQGARLLEQTLAWAQQKLEPTNTLQPILEKLYQAQTLSQQES
                     HQLFSAVVRGELKPEQLAAALVSMKIRGEHPNEIAGAATALLENAAPFPRPDYLFADI
                     VGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNAD
                     KSRQALDELGVCFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGV
                     YSPELVLPIAETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGEIKSYQLTAEDF
                     GLTPYHQEQLAGGTPEENRDILTRLLQGKGDAAHEAAVAANVAMLMRLHGHEDLQANA
                     QTVLEVLRSGSAYDRVTALAARG"
     gene            complement(7427..8989)
                     /gene="trpE"
                     /locus_tag="ACHKBL_003201"
     CDS             complement(7427..8989)
                     /gene="trpE"
                     /locus_tag="ACHKBL_003201"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HFO5820036.1"
                     /translation="MQTQKPTLELLTCEGAYRDNPTALFHQLCEDRPATLLLESADID
                     SKDDLKSLLLVDSALRITALGDTVTIQALSGNGEALLALLDNALPAGVENEQSPNCRV
                     LRFPPVSPLLDEDARLCSLSVFDAFRLLQSLLNVPNEEREAMFFGGLFSYDLVAGFED
                     LPQLAAENNCPDFCFYLAETLMVIDHQKKSTRIQASLFAPNEEEKQRLTARLNELRQQ
                     LTEAAPPLPVVSVPHMRCECNQSDEEFGGVVRSLQKAIRAGEIFQVVPSRRFSLPCPS
                     PLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQIEIYPIAGTRPRGR
                     RADGSLDRDLDSRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSRYVADLTKVDR
                     YSYVMHLVSRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYG
                     GAVGYFTAHGDLDTCIVIRSALVENGIATVQAGAGVVLDSVPQSEADETRNKARAVLR
                     AIATAHHAQETF"
     misc_feature    complement(9011..9104)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00513"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Trp leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00513"
     gene            complement(9081..9125)
                     /gene="trpL"
                     /locus_tag="ACHKBL_003202"
     CDS             complement(9081..9125)
                     /gene="trpL"
                     /locus_tag="ACHKBL_003202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon leader peptide"
                     /protein_id="HFO5820037.1"
                     /translation="MKAIFVLKGWWRTS"
     gene            9263..10144
                     /gene="rnm"
                     /locus_tag="ACHKBL_003203"
                     /gene_synonym="trpH"
                     /gene_synonym="yciV"
     CDS             9263..10144
                     /gene="rnm"
                     /locus_tag="ACHKBL_003203"
                     /gene_synonym="trpH"
                     /gene_synonym="yciV"
                     /EC_number="3.1.13.-"
                     /EC_number="3.1.3.97"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase AM"
                     /protein_id="HFO5820038.1"
                     /translation="MSDTNYAVIYDLHSHTTASDGCLTPEALVHRAVEMRVGTLAITD
                     HDTTAAISPAREEISRSGLALNLIPGVEISTVWENHEIHIVGLNIDITHPLMCEFLAQ
                     QTERRNQRAQLIAERLEKAQIPGALEGAQRLAQGGAVTRGHFARFLVECGKASSMADV
                     FKKYLARGKTGYVPPQWCTIEQAIDVIHHSGGKAVLAHPGRYNLSAKWLKRLVAHFAE
                     HYGDAMEVAQCQQLPNERTQLATLARQHHLWASQGSDFHQPCPWIELGRKLWLPAGVE
                     GVWQLWEQPQNTTEREL"
     gene            10141..10761
                     /gene="yciO"
                     /locus_tag="ACHKBL_003204"
     CDS             10141..10761
                     /gene="yciO"
                     /locus_tag="ACHKBL_003204"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HFO5820039.1"
                     /translation="MSQFFYIHPDNPQQRLINQAVEIVRKGGVIVYPTDSGYALGCKI
                     EDKNAMERICRIRQLPDGHNFTLMCRDLSELSTYSFVDNVAFRLMKNNTPGNYTFILK
                     GTKEVPRRLLQEKRKTIGMRVPSNPIAQALLEALGEPMLSTSLMLPGSEFTESDPEEI
                     KDRLEKQVDLIIHGGYLGQKPTTVIDLTDDTPVVVREGVGDVKPFL"
     gene            10789..12678
                     /gene="yciQ"
                     /locus_tag="ACHKBL_003205"
     CDS             10789..12678
                     /gene="yciQ"
                     /locus_tag="ACHKBL_003205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HFO5820040.1"
                     /translation="MAGIYRCILLLIVGLFFSSLSYPKNTEIPFYEEGISLFDVEATL
                     QPDGVLDIKENIHFQARNQQIKHGFYRDLPRLWMQPDGDAALLNYHIVGVIRDGIPEP
                     WHLDWHIGLMSIVVGDKQRFLPQGDYHYQIHYQVKNAILREGDSDLLIWNVTGNHWPF
                     EIYKTLFSLKLPDIAGNPFSEIDLFTGEEGDTYRNGRILEDGRIESRDPFYREDFTVL
                     YRWPHALLGNAPAPQTTNIFSHLLLPSTSSLLICFPSLFLACGWLYLWKRRPQFTPVD
                     VIETDVIPPDYTPGMLRLDAKLVYDDKGFCADIVNLIVKEKIHLEDHYDKNQQILIRV
                     NEGATRNNAVLLPAEQLLLEALFRKGDKVVLTGRRNRVLRKAFLRMQKFYLPRKKSSF
                     YRPDAFLQWGGMAILAVILYGNLSPVGWEGMSLVGDMFIMICWLLPFLFCSLELLFAR
                     DDDKPCVNRVIITLFLPLICSGVAFYSLYINVGDVFFYWYMPAGYFSAVFLTGYLTGM
                     GYIFLPKFTQTGQQRYAHGEAIVNYLARKEAATHSGRRRKGETRKLDYALLGWAISAN
                     LGREWALRIAPSLSSAICAPEIARNGVLFSLQTHLSCGAYTSLLGRSYSGGGSGAGGG
                     GGGGW"
     gene            12891..13766
                     /gene="rluB"
                     /locus_tag="ACHKBL_003206"
     CDS             12891..13766
                     /gene="rluB"
                     /locus_tag="ACHKBL_003206"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HFO5820041.1"
                     /translation="MSEKLQKVLARAGHGSRREIESIIEVGRVSVDGKIAKLGDRVEI
                     TPGLKIRIDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGAR
                     WIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGV
                     QLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDI
                     PLPKGLPRGGWTELDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHC
                     QVSGGRRSGGRNNNG"
     gene            13936..>14486
                     /locus_tag="ACHKBL_003207"
     CDS             13936..>14486
                     /locus_tag="ACHKBL_003207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001599750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5820042.1"
                     /translation="MIRYSGIFLSLLMLSGCATEALKNEQAETVITAGNKAVAANASF
                     IKQYYASRENFLVNFYATNPDCALRPDYTIDIGGDGNGGKLCQRSGPNTKQVQLVPFE
                     KEKLLASMEVINSMSAYVELLTKDMATTESQQVALLKQALDHANNAQTLLGVSHSKDL
                     AQSAAAVKDLVNYFNELYIIKVNS"
BASE COUNT         3519 a         3518 c         3711 g         3738 t
ORIGIN      
        1 tcatttgtta ctccaatgta ggtatattcg tcacgttttt ataaccataa cgacggagcg
       61 gatatgaaaa agttaacagt ggcggctttg gcagtaacaa ctcttctctc tggcagtgcc
      121 tttgcgcatg aagcaggcga attttttatg cgtgcaggtt ctgcaaccgt acgtccaaca
      181 gaaggtgctg gtggtacgtt aggaagtctg ggtggattca gcgtgaccaa taatacgcaa
      241 ctgggcctga cgtttactta tatggcgacc gataacattg gtgtggaatt gctggcagcg
      301 acgccgttcc gccataaaat cggcacccgg gcgaccggcg atattgcaac cgttcatcat
      361 ctgccgccaa cactgatggc gcagtggtat tttggtgatg ccagcagcaa attccgtcct
      421 tacgttgggg caggtattaa ctacaccacc ttctttgata atggatttaa cgatcatggc
      481 aaagaggcgg ggctttccga tctcagtctg aaagattcct ggggagctgc cgggcaggtg
      541 ggggttgatt atctgattaa ccgtgactgg ttggttaaca tgtcagtctg gtacatggat
      601 attgatacca ctgccaatta taaactgggc ggtgcacagc aacacgatag cgtacgcctc
      661 gatccgtggg tgtttatgtt ctcagcagga tatcgttttt aattccgcac aaaaatgacc
      721 ccgtaattac ggggtcaata aggacatggt ataaagggta ttatttcttc gcttctacgc
      781 catcagtttc agagcgaatt aagaatttct cagttgtttg cggaatatgc tgaattagcc
      841 agtcggccat gtgcttttca tcatttaaaa tcgcttcgat aatcggaatt gaggctgtat
      901 caccggcatt ttttgctgct gctaagagtg aggtataaca ggcgatttca aattgctcga
      961 agacatatcc gctaatagag cctttgacta tttcatcaga agggaatata ccaccgattg
     1021 actgcccaag tgcagccatt ttactcatgg aatctttaat gactgaacgt gaaatgtcat
     1081 tacgatcaag aatagtttcc agttgaacaa tctggttttt ggtttcacta agatgttgtt
     1141 caatacgagc gcgtagttca ggataattat ctatacgact ggccatggat tcaagcattg
     1201 attcggcttg cttttccatt gcgtgggcgt cacgtaacca gtcatgataa tgttcaatac
     1261 gattcatgaa aatgtcctct ttataaaatg actaaaagtt aaagttcata tttcaggctt
     1321 tattttcggc tttcttattt acgttattaa gggccagatc agtcaatttg atgtcggtgg
     1381 ccttttcttc ttccagggtc tctttcagaa gcttcgctgc tttacgataa cctaattgtt
     1441 cagccagcgt cgctaatgtc ccgtaactgg caatctcata atgctcgact ttctgtgctg
     1501 cggcaatcag tgcggcatca cgcacttcgt ttttctcggt actttcgatg acctcattag
     1561 cttcttcaat aagaccttcc attgccacac atttcatgcg cttaattttc agattcgatt
     1621 ctgattccac aacttgatca atacgttcaa tctgtccata tgtttcctcg aggtgcgcat
     1681 gaaaagcctg acttaatttt tcgtttgatg ttgctcttgc gagttttgcc agtgcccggg
     1741 ttaattgttt ttctgcgctg taggtatctg aaagcaggtg aataaataca tcttcaatgg
     1801 tcttcatatt catatttttc tccagtgaaa tcactgcgag ccatacggcc cgcagatgca
     1861 attatcttta tcagctgata ttaataatta acggtgaatc aggatttgcc tgatttatta
     1921 ccaccgcttt gttgaccgcc ttttttaccc gcttcagatg cgcgttgcgg atcattttta
     1981 aaattaccgc cgctatgctg accgccttta cggccagcgt cggatgcctt ttcacggtct
     2041 tcggcgaaat ttcctgaacc accacgatgt tcggccatgt tatttctccc gttgcgttgc
     2101 attgtttcat taatatgggt gttgtgtgtc gacactcatt aaaattagtc gctaatgaaa
     2161 attagtcaaa ttaagcgcaa cgagaaaaaa gagggaaaat atattatgag gaacattctg
     2221 gatatattaa caattgcctg aggaataagt gacttagaga aaattattga ttttactggt
     2281 gttatgttgc gggattaatt tgtttgatta tattactgtt gggcggaaaa aagacgtaag
     2341 ttgacgttcg acaggggcaa gcgaaacggt aaaaagataa ataataaatg aatttaggat
     2401 ttttccagct tcgttaaaga aagttaaaat gccgccagcg gaactggcgg ctgtgggatt
     2461 aactgcgcgt cgccgctttc atcggttgta caaaagcttt cagtgccgcc agcattttct
     2521 ctggctcatt aatatgttgc tcgatgattt taacgatggc cgaaccagaa atcgcgcccg
     2581 cagctcctgc atcaatcgct gcttttacct gatccggggc ggaaatacca aagccctgca
     2641 atggaggcgc agcattgtat tctttcagct tcgtgaccag atgattgagg ggtaacgcgg
     2701 cgcggttttc tgcgccggtc acgcccgcac gtgacaataa ataggtgtat ccacgaccgt
     2761 aggaggctat ctggcgcagc aaatcatcgt cggcattcgg cgggcagata aagataggtg
     2821 caagattatg acgcaacgcg gcctggcgga agggagcgga ctcttccact ggcacatcgg
     2881 caaccagcac cgaatcgacg ccgactttct cgcactgggc ataaaactca tcaatgcctt
     2941 tgttaaacac caggttggca tacatcagca ggccaatggg aatggtcggg tgtttctggc
     3001 gaatcagtgc cagcatttca aaacattgtg ccggagtcac acctgccgca aaggcgcgca
     3061 gagtggcgtt ttgaatcgtc gggccatccg ccagtgggtc ggagaagggg atacctaact
     3121 ccagcgcgtc agcaccggct tcaattagcg tgtcgataat tttcagcgac tgctcaatgc
     3181 ccggatcacc gagcgtgacg aaaggaacga atgcgccttc tttgcgctcc ttcaactggg
     3241 caaacagaga ttcgtagcgt tccatcagat ttcccctcgt gctttcaaaa tatcgtgaac
     3301 ggtgaagatg tctttatcgc cgcgaccgga aaggttaacc accagtagct gctctttttc
     3361 cgggttttcg cgcatcattt tcagcgcatg ggccagggcg tgtgaggact ccagcgccgg
     3421 gataatccct tcgtgcaggc agagcgtttt gaaggcttcc agtgcttcat catcggtaat
     3481 tgacacgtaa tcagcgcgtc cagtgctgtt aagatacgcg tgttgtgggc cgacagacgg
     3541 gaaatccagt ccggcggaga tggagtaaga ttcttcaatc tgcccgtctt cggtttgcat
     3601 catcggcgct ttcataccga aatagatgcc cacgcgaccg tgttttaacg gtgcgccgtg
     3661 ctcgccagtt tcgataccgt gaccacctgg ctccacacca atcaggccga cgttggtttc
     3721 attgatgaaa tcagcaaaca tgccgatggc attcgaaccg ccgccaacac aggcgataac
     3781 agcatccggc aggcgacctt ctctttccag aatctgcgct ttcgtttctt cgccaatcat
     3841 ccgctgaaac tcacgcacaa tggtcgggta aggatgcggg ccagccgcgg tgcccagcat
     3901 atagtgcgcg gtttcataac tgccggacca gtcgcgtagc gcctcattac aggcatcttt
     3961 cagcgtcgcg gaaccgctat gcaccgggat cacttccgca cccattaaac gcatacgaaa
     4021 aacgttaggc gactggcgtt caacgtcttt ggcacccata taaatgcgac atttcaggcc
     4081 gagcagggcg ctggcaaggg ccgacgccac gccatgctga ccggctccgg tttcggcgat
     4141 gatttccgtt ttacccatcc gcttcgccag caacgcctgg ccgagcactt ggttggtttt
     4201 atgcgcgccg ccgtgcagca aatcttcgcg cttgagatac agcgtggtgt tagtgccagc
     4261 cgtaatgttc tggcatttgg tgagtgcggt tggacgcccg gcatagtttt tcagcaggtc
     4321 gttgaactga gcctgaaatt caggatcttt ttgcgcgcta acaaaagctt cttccagctg
     4381 gcgcagagca ggcatcagga tttgtggcac gtacatgccg ccaaactcac caaaatacgg
     4441 gttaagtaat gttgtcattg ttcctttcct taatatgcgc gcagcgtctg gaaaaccgag
     4501 gccaaaagac gtgcgtcttt gatgcccggt tgcgactcta cagcagaatt aaaatcaagt
     4561 ccggcgcagc cggtttgtgc cgcttccacg cagttatctg cgcctaagcc ccccgccagc
     4621 agaacgttgc caagcgattg accgtttaac agtgaccagt cgaaacgttg accgcttcca
     4681 ccctggccgt tgtctaaaac atatttatca acgtgctgaa gctcgcgggc gggcagggtt
     4741 tcaccgacgc ttaatgcttt ccagatggca acgtgtgctg gcagagcttc acgcagcgta
     4801 tcgatataca gctgatcttc attaccatgc agttgcactg ccgccagcga taacacctta
     4861 gctttgtcca ccacatcggc aatatcgtga ttgcggaaca cgccaacata ctgcaacggt
     4921 gccgcagcca tcacttcctg cgcctgttca acgttgacgc aacgcggtga tgtcgcaaca
     4981 aaaatcaacc caccgtaaat cgcgcccgcg tcataagctg ctttagcatc ttgcccacgc
     5041 gtcaggccac atactttatt ctcacccagc aatacccggc gcacggcggc gttcaaatcg
     5101 tcatgggcca tcaacgccga accaatcaga aaaccgttag cgaagtggct taactcgcgc
     5161 acctgagcgt aagtattgat gccggattcg ctgattaccg tcacgttgtg ccccagtttc
     5221 ggcgcaagct cgcgggtacg gttgagatca atcgacaaat cacgcagatc gcggttgttg
     5281 atgccaacga cctttgcccc caatgcaatg gcgcgctcca gttcctcttc attactgact
     5341 tcggtcagca cacccatctc cagactgtgg gcgacggcgg caaggtggcg atattgttcg
     5401 tcatccagta ctgaaagcat taataagcag gcatcggcct ggtaatagcg cgccagatag
     5461 atctggtaag ggtcgataat aaagtcttta cataaaatcg gctgcggggc gatttggctg
     5521 acgatggaga ggaaatcaaa gctcccctga aaatatttct catcagtcag caccgaaatt
     5581 gccgaagcgt aatttctgta gacggttgca atgtgtgccg gatcaaaatc atcgcggatc
     5641 acgccttttg acggtgacgc ttttttacac tccagaataa acgccgtgcg tgcaccctgt
     5701 agcgcatcat aaaaatgtcg cgtgctcggc tgaacctcat tctgaaaact ggccagcggt
     5761 tgctgctgtt tgcgggcttc tacccaaatc gccttgtctg cgacgatttt cgctaaaacg
     5821 gtttgcatca tttaccctcg tgccgccagt gcggtgactc tgtcgtaagc ggaaccactg
     5881 cgcagtacct caagaacggt ttgcgcattg gcttgcagat cttcatggcc atgcaggcgc
     5941 attaacatgg cgacgttcgc cgcgacggct gcttcatggg cggcgtcgcc tttaccttgt
     6001 aacaagcgtg ttaaaatgtc acggttttct tccggtgttc cgcctgccag ttgctcctgg
     6061 tggtagggtg tcaggccaaa atcttctgca gttagctgat agctcttaat ttcaccgtca
     6121 tgcagttcgg caacgattgt cggcgcgtgt aatgaaactt catccatccc gccgctgtgc
     6181 actaccgcag cgcgttgata ccccagcacg cgcaaggttt cggcaatcgg cagcaccagt
     6241 tccggactat aaacaccaat tagcgccagc ggcggatgcg ccgggttaat caatggcccc
     6301 aacacgttga acagggtgcg ggttttcagt tgctggcgaa ccggcatcgc gtggcggaat
     6361 ccggtgtgat atttcggcgc aaagaggaaa cagacgccta actcatccag cgcctggcgc
     6421 gatttatcgg cgttcatatc aagattaata ccgaacgccg ccagcagatc ggacgagccg
     6481 gatttactgg agacgctgcg gttaccgtgt ttcgccactt tcagcccaca ggccgcggcg
     6541 acaaacgcac tggcggtaga aatattgatg ctgttgctgc cgtcaccgcc agtaccgacg
     6601 atatcggcaa acagataatc cgggcgcggg aacggcgcgg cgttttccag tagcgcggtt
     6661 gctgctccgg cgatctcgtt cgggtgctcg ccgcgaattt tcatgctcac cagcgccgcc
     6721 gccagttgtt ccggcttcag ctcgccacgc accaccgctg aaaacagctg gtgactttct
     6781 tgttggctaa gcgtctgtgc ctgatacagt ttttccagaa tcggttgcag cgtgttggtt
     6841 ggctcaagtt tctgctgcgc ccaggccagc gtttgttcca gcaggcgagc gccctgggta
     6901 gtaagaatgg attccggatg gaactggaaa ccgcaaacgc gatctgcatc gtgacgcacc
     6961 gccatcacca tgccattaaa atgggcgttg atggttaaac ctgccgggat attactgcca
     7021 accagcgagt gataacgcgc caccggcaat gggtttgtta atccggcaaa catcgcctga
     7081 ctgtcatgtt caatgctcga cgctttaccg tgaagaattt cgcccgcctg accgacatag
     7141 cccccgtaag cttcgacaat tgcctgatgt ccgaggcaaa tgccaataat tggcagcttg
     7201 ccacgcaagc gggtgaggag ttccggcata caaccggctt cgctcggcac accggggcca
     7261 ggagaaagca tcagcaccgg attgctcatc gtcgccaggc gttcaattaa ggtctgcgcc
     7321 ggaacatggt tgcggtaaat caccacgtta tgaccattgc tgcgcaactg atctgccagg
     7381 ttgtacgtaa aagagtcgat attatcgagc agcagaatgt cagccatcag aacgtctcct
     7441 gtgcatgatg cgcggtggca atagcgcgca gtacagcgcg ggctttatta cgggtttcgt
     7501 cggcttccga ctgcggaaca gaatcaagga ctacgccagc accggcttgc acggtggcga
     7561 taccgttttc caccagcgcc gagcggatca caatgcaggt gtcgagatcg ccatgcgcgg
     7621 taaaataacc taccgcgccg ccgtagctgc cgcgtcgacg accttctgct tcggcaatta
     7681 actgcatagc gcgcactttc ggcgcaccgc ttaatgtccc catattcata caggcgcggt
     7741 aagcgtgcag ggcgtcgaga tcgtggcgca gctcaccgac cacgcgggag acgaggtgca
     7801 tcacataaga ataacggtca actttggtga gatcggcgac gtagcgacta ccgggagtgc
     7861 aaatacgcgc caggtcatta cgggcgagat ccactagcat cagatgttca gaaagctctt
     7921 tatgatcggt acgcatttcc agttcgatgc ggctgtcgag gtctctgtcc agtgaaccat
     7981 cggcacgacg accgcgtgga cgtgttccgg caatcgggta gatctcaatc tggcggctgg
     8041 tggcgtcata cttgagcgaa ctttccggcg acgcgccaaa cagggtgaaa tcattatcct
     8101 gcataaaaaa catgtacggg ctgggattac tctttttcag cacgtaatag gccgccagcg
     8161 gtgacgggca gggcagagag aaacggcgag atggcaccac ctggaaaatt tctccggcgc
     8221 gaatcgcttt ttgcagcgaa cgcactacgc caccgaactc ttcatcactc tggttacatt
     8281 cacaacgcat atgcggcacg gaaaccaccg gcagcggcgg cgcggcttcg gtcagttgct
     8341 gacgtagttc gttcaggcga gcagtgagac gttgtttttc ttcttcattc ggagcaaaca
     8401 ggctggcctg aatacgggtg ctttttttct gatggtcaat caccatcagc gtttcagcga
     8461 gataaaaaca gaaatcagga cagttatttt ccgctgccag ttgtggtaaa tcttcaaatc
     8521 ccgccacaag gtcataagag aacagaccgc cgaagaacat tgcttctcgt tcttcattcg
     8581 gtacattcaa cagactctgc aataagcgga aagcgtcaaa aaccgaaagg gagcataagc
     8641 gagcgtcttc atccagcagt ggactgacag gggggaagcg cagcacacgg cagtttggtg
     8701 attgttcatt ttccacaccc gcaggcaggg cgttatccag tagtgccagc agggcttcgc
     8761 cattgccgga aagtgcctgg attgtgacag tgtcacctaa agctgtaatg cgcagcgcac
     8821 tgtctaccag cagcaggctt tttaaatcat ctttgctgtc gatatctgcg gattccagta
     8881 gcagcgttgc tggacgatcc tcacacaact ggtgaaaaag cgcggtggga ttgtcgcgat
     8941 aagcgccttc gcaggttagc agttcgagag tcggtttttg tgtttgcatt gttattctct
     9001 aattttgttc aaaaaaaagc ccgctcatta ggcgggctgg gtatctgatt gctttacgca
     9061 tggtgaatac actgcccgtt tcaggaagtg cgccaccaac ctttcagtac gaaaattgct
     9121 ttcattgtcg ataccctttt tacgtgaact tgcgtactag ttaactagtt cgatgattaa
     9181 ttgtcaacag ctcatttcag aatatttgcc agaaccgtta tgatgtcggc gcaaaaaaca
     9241 ttatccagaa cgggagtgcg ccttgagcga cacgaattac gcagtgattt acgacctgca
     9301 cagccatacc acagcttccg atggctgcct gacgccagaa gcattggtgc accgtgcagt
     9361 cgagatgcgc gtcggcaccc tggcgatcac cgaccatgac accacagctg caatctcacc
     9421 agcaagagag gaaatttcac gttcgggact ggcgctgaat cttattcccg gcgtggaaat
     9481 ttccacggtc tgggaaaatc atgaaattca tattgtcggg ctgaatattg atattactca
     9541 tccgctaatg tgtgagttcc tcgcgcagca gacagaacgg cgcaatcagc gggcgcagct
     9601 gattgccgaa agacttgaaa aagcgcaaat acctggggca ctggaaggcg cgcaacgact
     9661 ggcgcagggc ggtgcggtga cgcgcggtca ttttgctcgt ttccttgtgg agtgcggcaa
     9721 agccagttca atggcggatg tctttaaaaa gtatctggcg cgcgggaaaa ccggatacgt
     9781 tccgccacag tggtgtacaa tagaacaagc tattgatgtc attcatcatt ctggcggtaa
     9841 ggcggtgcta gctcatcctg ggcggtacaa tcttagtgct aaatggctga aaagattggt
     9901 tgcgcatttt gccgaacact acggcgacgc gatggaagtc gcgcagtgcc agcaattgcc
     9961 caatgaacgc acccagctgg cgacacttgc gcgtcagcat catttatggg catcacaagg
    10021 atctgatttt catcagccat gcccgtggat cgaactgggt cgtaaactct ggctgcccgc
    10081 aggcgttgaa ggcgtctggc agctatggga acagccgcag aacaccacag agagggaatt
    10141 atgagccagt ttttttatat tcatcctgat aacccgcagc aacgcctgat caaccaggcg
    10201 gtggagatcg tgcgtaaagg cggggtgatt gtttatccaa ctgattccgg ctatgcgctc
    10261 ggctgtaaaa ttgaagataa aaacgcgatg gagcgtattt gtcgaattcg ccagctgccg
    10321 gacggtcaca actttaccct gatgtgtcgc gatctttctg agctctcgac ctattcattc
    10381 gtggataacg ttgcgtttcg tttgatgaaa aacaacacgc cgggcaacta caccttcatc
    10441 ctgaaaggga cgaaggaggt gccacgccgt ctgttgcagg aaaaacgcaa aaccatcggt
    10501 atgcgtgtgc cttctaaccc gattgcgcag gcgctgcttg aagcattggg cgaaccgatg
    10561 ctttccacat cactgatgct gccaggcagc gaatttaccg aatccgatcc ggaagaaatt
    10621 aaagaccgtc tggaaaaaca ggtggacttg attattcacg gcggctatct cgggcagaaa
    10681 ccaacaacgg ttattgatct tactgatgat acgccggtcg tggtgcgtga aggcgtaggt
    10741 gatgtgaagc ctttcttata acaaattgcg ttccacggat ggaagactat ggcagggata
    10801 tatcgctgca ttttgctgtt gatagttggg ctttttttct cttcgttgag ttatccgaaa
    10861 aacacggaga tcccttttta tgaagaaggg atctcgctct ttgatgttga agccactctg
    10921 caaccggatg gggtgctcga catcaaagaa aatattcatt ttcaggcgcg aaatcagcag
    10981 attaagcacg gattttatcg tgatttacca cgactctgga tgcagcctga tggggacgct
    11041 gcactgctga actatcatat tgttggcgtc atccgtgatg gtattcctga accctggcat
    11101 cttgactggc atatcgggtt aatgagtatt gtcgtgggcg ataaacaacg tttcttgcct
    11161 caaggcgact atcattatca aattcattat caggttaaaa atgctatcct gcgtgagggg
    11221 gattctgatc tgctaatctg gaacgtgacc ggtaaccact ggccgtttga aatttataag
    11281 accctatttt cactcaagtt gcctgatatt gcgggtaatc catttagcga aatcgatctc
    11341 tttactggag aagagggcga cacatatcga aatggccgca tccttgagga cggaagaatt
    11401 gaatctcgcg atccgtttta tcgtgaagat ttcacggtcc tctaccgctg gcctcacgca
    11461 ttacttggta atgccccggc tccacaaacg acgaatattt tcagtcatct tcttttaccc
    11521 tcaacgtcat cgttgttaat ttgctttccg agtctgttcc tggcgtgtgg atggttatat
    11581 ctctggaagc gcaggccgca atttacgccg gtagatgtga ttgaaaccga tgtcattccg
    11641 ccagattaca cgcccggcat gttacgtctt gatgcgaaac tggtttacga cgataaaggt
    11701 ttttgcgccg atatcgtaaa tctgattgtg aaagaaaaaa ttcatctgga agatcattat
    11761 gacaagaacc agcaaatcct gattcgtgtt aatgaaggcg cgaccagaaa taatgcggta
    11821 ttactgcccg cagagcagtt attactggaa gcgttatttc gtaaaggcga taaggtcgtt
    11881 cttacgggga gacgcaacag agtcttacgc aaggcatttt tacggatgca gaaattttat
    11941 ctgccgcgta aaaagtcttc gttttaccga cctgatgcgt ttttgcaatg gggcggaatg
    12001 gcaatattgg cggtcattct ctacggtaac ctgagtcccg taggctggga aggaatgagt
    12061 ctggtgggcg atatgtttat tatgatctgc tggcttcttc cttttttatt ttgttccctt
    12121 gagcttttgt ttgcccgcga tgatgacaag ccttgcgtta atcgtgtcat catcactttg
    12181 tttttaccac tgatttgttc aggtgtggct ttttactctc tctacatcaa tgtcggagat
    12241 gtattctttt actggtatat gccagcgggt tattttagcg ctgttttcct gaccggttat
    12301 ctcactggca tggggtatat ttttctgcca aagtttaccc aaactgggca gcaacgttat
    12361 gcccacggtg aagctatcgt taactatctt gcgcgtaaag aggcagcaac acacagtggg
    12421 cggcgtagga aaggggaaac acggaaactg gattacgctt tgttaggttg ggctatatcg
    12481 gcaaatttgg ggagggaatg ggcgttacgc atcgcccctt cgctttcttc ggcgatttgc
    12541 gctccagaga ttgcccgtaa cggcgtttta ttctcattac agacgcacct gagttgcggg
    12601 gcctatacca gtttgttggg gcggagctat tctggtggtg gttctggcgc tggtggcgga
    12661 ggcggtggtg gctggtaatc aagctgatgt taatcgaaac aagctttaac cataaagata
    12721 gtgtagtatg ttgcgcctca aagccaggca agttaaaacg tcgagtcgtt tacttaaggc
    12781 ctgaagagtt caaacagact ttactatcag tgagtcagat gtaaattctg actcactgtt
    12841 gcacaaaagc atatcagacg cctgggaagg cgacaccaaa aggaagctct atgagcgaaa
    12901 agctacagaa agtgctggcg cgtgccggcc acggttctcg ccgtgaaatc gaatctatta
    12961 ttgaagtcgg tcgtgtgagt gttgatggca aaattgctaa actcggcgat cgcgttgaaa
    13021 ttacccctgg attgaaaatc cgtatcgatg gtcacctgat ttcggtacgt gagtccgctg
    13081 aacaaatttg tcgcgttctg gcctattaca aaccggaagg tgagttgtgt acccgtaacg
    13141 atccggaagg gcgtccaacg gtgtttgacc gtctgccaaa gctgcgtggc gcacgctgga
    13201 ttgccgtagg tcgcctggac gttaatacct gtggtctgct gctgttcact accgacggtg
    13261 aactggcaaa ccgtttaatg catccaagcc gtgaagttga acgtgaatac gccgtgcgcg
    13321 tatttggtca ggttgacgac gcgaaactgc gtgatttgag tcgtggcgtg cagctggaag
    13381 atggtccggc agcctttaaa accatcaagt tcagcggcgg cgaagggatc aaccagtggt
    13441 acaacgttac tctgaccgaa ggccgtaacc gtgaagttcg tcgtctgtgg gaagcggttg
    13501 gtgtgcaggt aagccgcctg atccgtgttc gttacggtga tatcccactg ccgaaaggtc
    13561 tgccacgcgg tggctggacg gagctggacc tcgcccagac taactatctg cgcgaactgg
    13621 tggagctacc gccagaaacc agctctaaag tcgctgtaga aaaagaccgt cgtcgtatga
    13681 aggcgaatca gattcgtcgt gcggtgaaac gtcactgtca ggtgagcggc ggtcgtcgtt
    13741 ctggtggacg taataataac ggttaattta agttaagtat aaaataaagg ccctgcgggg
    13801 ccttttcttc atttgaagta tgataaatag tcgcagtatt ttaatttaat ttcatacttg
    13861 cttatgaata ttaccaggaa atagccacat tactatttaa ttatctgaga taaactcaac
    13921 gtcaagaggt gggttatgat tagatatagt ggaatatttc tatcgctgtt aatgctttca
    13981 ggttgtgcaa ctgaagcatt gaaaaacgaa caagctgaaa ctgttattac tgcgggtaac
    14041 aaagcagtag cggcgaatgc aagtttcatt aaacaatact atgcttctcg tgaaaacttc
    14101 cttgttaatt tctacgcgac taatccggat tgtgccctgc gacctgatta taccattgat
    14161 ataggcggtg atgggaatgg cggaaaatta tgccagcgtt cagggccaaa tacaaaacaa
    14221 gtacaattag tccctttcga gaaagaaaaa ttactggcct caatggaagt cattaatagc
    14281 atgagcgctt atgtggaact tctgacaaag gatatggcaa ctacggaaag tcagcaagtg
    14341 gcattactta aacaggctct ggatcacgca aacaacgcac aaacactact tggtgtttca
    14401 catagcaaag atttagctca atctgcagca gcggttaaag atttggtaaa ttatttcaat
    14461 gagttatata taattaaagt caattc
//
LOCUS       DAZFLD010000127        14424 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.128, whole genome shotgun sequence.
ACCESSION   DAZFLD010000127 DAZFLD010000000
VERSION     DAZFLD010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14424
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.128"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            111..677
                     /locus_tag="ACHKBL_003208"
     CDS             111..677
                     /locus_tag="ACHKBL_003208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO5820043.1"
                     /translation="MFKGQKTLAALAISLLFTAPVYAADEGSGEIHFKGEVIEAPCEI
                     HQDDIDKEVELGQVTTSHINQSHHSDAVAVDLRLVNCDLENSNNGSGGKISKVAVTFD
                     SSAKTTGADPILNNTSTGEATGVGVRLMNKDQSNIVLGTATPDIDLAPTSSEQTLNFF
                     AWMEQIDQATPVTPGAVTANATYVLDYK"
     gene            728..3187
                     /gene="ybgQ"
                     /locus_tag="ACHKBL_003209"
     CDS             728..3187
                     /gene="ybgQ"
                     /locus_tag="ACHKBL_003209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFO5820044.1"
                     /translation="MDTVNIYRLSFISCLVMAMPCALAVEFNLNVLDKSMRDRIDISL
                     LKGKGVIVPGEYFVSVAVNNNQISNGQKIDWKKNGDQTIPCINDLLVDKFGLKPEVRQ
                     SLPRLNQCVDFSSRPEILFIFDQASQQLNITIPQAWLAWHSDNWTPPSTWKEGVAGVL
                     MDYNLFASSYRPQDGSNSTNLNAYGTAGINAGAWRLRSDYQLNQTDSDDNHEQSGEIS
                     RTYLFRPLPQLGSKLTLGETDFSSNIFDGFSYTGAALASDDRMLPWELRGYAPQISGI
                     AQTNATVTVSHSGRVIYQKKVPPGPFIIDDLNQSVQGTLDVKVTEEDGRANNFQVSAA
                     STPFLTRQGQVRYKLAAGQPRTSMSHQTENETFFSNEVSWGMLSNTSLYGGLLISGDD
                     YHSAAMGIGQNMLWLGALSFDVTWASSQFDTQQDERGLSYRFNYSKQVDATDSTISLA
                     AYRFSDRHFHSYANYLDHKYNNSDAQDEKQTISLSVGQPITPLNLNLYANLLHQTWWN
                     ADASTTANITAGFNVDIGNWRDISISTSFNTTHYEDKDRDNQIYLSISLPFGNGGRVG
                     YDMQNSSHSTTHRMSWNDTLDERNSWGMSTGLQSDRPDNGAQVSGNYQHLSSAGEWDI
                     SGTYAANDYSSVSSSWSGSFTATQYGAAFHRRSSTNEPRLMVSTDGVADIPVQGNLDY
                     TNHFGIAVVPLISSYQPSTVAVNMNDLPDGVTVAENVIKETWIEGAIGYKSLASRSGK
                     DVNVIIRNASGQFPPLGADIRQDNSGISVGMVGEEGHAWLSGVAENQQFTVIWGDSQR
                     CSLHLPEHMEDTANRLILPCH"
     gene            3202..3933
                     /gene="ybgP"
                     /locus_tag="ACHKBL_003210"
     CDS             3202..3933
                     /gene="ybgP"
                     /locus_tag="ACHKBL_003210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFO5820045.1"
                     /translation="MTFMKGLPLLLLVASLCSHAALQPDRTRIVFNANDKATSLRVDN
                     RSDKLPYLAYSWLENEKGEKSDDLLVALPPIQRLEPKATTQVRIVKQASTTKLPGDRE
                     TLFFYNMREIPPSPEKNSGHAVLQVAIQSRIKVFWRPAALRKKAGEKVELQLQVSQQG
                     NQLTLKNPTAYYLTIAYLGRNEKGVLPGFKTVMVAPFSTVITNTGSYSGSQFYLGYMD
                     DYGALRMTTLNCSGQCRLQAVEAKK"
     gene            3930..4991
                     /gene="ybgO"
                     /locus_tag="ACHKBL_003211"
     CDS             3930..4991
                     /gene="ybgO"
                     /locus_tag="ACHKBL_003211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO5820046.1"
                     /translation="MSAGKGVLLVICLVFLPLKSALALNCYFGTSGGTVEKSEAIQPF
                     AVPGNAKPGDKIWESDDIKIPVYCDNNTNGNFESEHVYAWVNPYPGVQDRYYQLGVTY
                     NGVDYDASLGKSRIDTNQCIDSKNINIYTPEQIIAMGWQNKICSGDPANIHMSRTFLA
                     RMRLYVKIREIPPHDYQSSLSDYIVVQFDGAGSVNEDPTAQNLKYHITGLENIRVLDC
                     SVNFSISPETQVIDFGKFNLLDIRRHTMSKTFSIKTTKSQNDQCTDGFKVSSSFYTEE
                     TLIEEDKALLIGNGLKLRLLDENSSPYTFNKYSEYADFTSDMLIYEKTYTAELSSIPG
                     TPIEAGPFDTVVLFKINYN"
     gene            5144..6190
                     /gene="abrB"
                     /locus_tag="ACHKBL_003212"
     CDS             5144..6190
                     /gene="abrB"
                     /locus_tag="ACHKBL_003212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFO5820047.1"
                     /translation="MPVLQWGMLCVLSLLLSIGFLAVHLPAALLLGPMIAGIIFSMRG
                     ITLQLPRSAFLAAQAILGCMIAQNLTGSILTTLAVNWPIVLAILLVTLLSSAIVGWLL
                     VRYSSLPGNTGAWGSSPGGAAAMVAMAQDYGADIRLVAFMQYLRVLFVAGAAVLVTRM
                     MLGDNAEAVNQQIVWFPPVSINLLLTILLAVVAGTVGCLLRLPSGTMLIPMLAGAVLQ
                     SGQLITIELPEWLLAMAYMAIGWRIGLGFDKQILLRALRPLPQILLSIFALLAICAGM
                     AWGLTRFMHIDFMTAYLATNPGGLDTVAVIAAGSNADMALIMAMQTLRLFSILLTGPA
                     IARFISTYAPKRSA"
     gene            complement(6187..6978)
                     /gene="nei"
                     /locus_tag="ACHKBL_003213"
     CDS             complement(6187..6978)
                     /gene="nei"
                     /locus_tag="ACHKBL_003213"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000703 - oxidized pyrimidine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
                     /protein_id="HFO5820048.1"
                     /translation="MPEGPEIRRAADNLEAAIKGKPLTDVWFAFPQLKPYQSQLIGQH
                     VTHVETRGKALLTHFSNDLTLYSHNQLYGVWRVVDTGEEPQTMRVLRVKLQTVDKTIL
                     LYSASDIEMLTPEQLITHPFLQRVGPDVLDPNLTPEVVKERLLSPRFRNRQFAGLLLD
                     QAFLAGLGNYLRVEILWQVGLTGNHKAKDLNAAQLDALAHALLDIPRFSYATRGQVDE
                     NKHHGALFRFKVFHRDGELCERCGGIIEKTTLSSRPFYWCPGCQH"
     gene            complement(7014..7748)
                     /gene="pxpA"
                     /locus_tag="ACHKBL_003214"
     CDS             complement(7014..7748)
                     /gene="pxpA"
                     /locus_tag="ACHKBL_003214"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HFO5820049.1"
                     /translation="MKIDLNADLGEGYASDAELLTLVSSANIACGFHAGDAQTMQACV
                     REAIKNGVAIGAHPSFPDRENFGRSVMQLPPETVYAQTLYQIGALATIARAQGGVMRH
                     VKPHGMLYNQAAKEAQLADAIARAVYACDPALILVGLAGSELIRAGKQYGLTTREEVF
                     ADRGYQADGSLVPRSQPGALIENEEQALAQTLEMVQHGRVKSITGEWATVTAQTVCLH
                     GDGEHALAFARRLRSTFAEKGIVVAA"
     gene            complement(7738..8670)
                     /gene="pxpC"
                     /locus_tag="ACHKBL_003215"
     CDS             complement(7738..8670)
                     /gene="pxpC"
                     /locus_tag="ACHKBL_003215"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpC"
                     /protein_id="HFO5820050.1"
                     /translation="MLKIIRAGMYTTVQDGGRHGFRQSGISHCGALDMPALRIANLLV
                     GNDANAPALEITLGQLTVEFETDGWFALTGAGCEARLDDNAVWTGWRLPMKAGQRLTL
                     KRPQHGMRSYLAVAGGIDVPPVMGSCSTDLKVGIGGLEGRLLKDGDRLPIGKSKRDFM
                     EAQGVKQLLWGNRIRALPGPEYHEFDRASQDAFWRSPWQLSSQSNRMGYRLQGQILKR
                     TTDRELLSHGLLPGVVQVPHNGQPIVLMNDAQTTGGYPRIACIIEADMYHLAQIPLGQ
                     PIHFVQCSLEEALKARQDQQRYFEQLAWRLHNEN"
     gene            complement(8664..9320)
                     /gene="pxpB"
                     /locus_tag="ACHKBL_003216"
     CDS             complement(8664..9320)
                     /gene="pxpB"
                     /locus_tag="ACHKBL_003216"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HFO5820051.1"
                     /translation="MQRARCYLIGETAVVLELEPPVTLASQKRIWRLAQRLVDMPNVV
                     EAIPGMNNITVILRNPESLALDAIERLQRWWEESEALEPESRFIEIPVVYGGAAGPDL
                     AVVAAHCGLSEKQVVELHSSVEYVVWFLGFQPGFPYLGSLPEQLHTPRRAEPRLLVPA
                     GSVGIGGPQTGIYPLATPGGWQLIGHTSLSLFDPARDEPILLRPGDSVRFVPQKEGVC
                     "
     gene            complement(9343..10086)
                     /gene="ybgI"
                     /locus_tag="ACHKBL_003217"
     CDS             complement(9343..10086)
                     /gene="ybgI"
                     /locus_tag="ACHKBL_003217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radiation resistance protein YbgI"
                     /protein_id="HFO5820052.1"
                     /translation="MKNTELEQLINEKLNSAAISDYAPNGLQVEGKETVQKIVTGVTA
                     SQALLDEAVRLGADAVIVHHGYFWKGESPVIRGMKRNRLKTLLANDINLYGWHLPLDA
                     HPELGNNAQLAALLGITVMGEIEPLVPWGELTMPVPGLELASWIEARLGRKPLWCGDT
                     GPEVVQRVAWCTGGGQSFIDSAARFGVDAFITGEVSEQTIHSAREQGLHFYAAGHHAT
                     ERGGIRALSEWLNENTDLDVTFIDIPNPA"
     gene            10357..11838
                     /gene="dtpD"
                     /locus_tag="ACHKBL_003218"
     CDS             10357..11838
                     /gene="dtpD"
                     /locus_tag="ACHKBL_003218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide permease DtpD"
                     /protein_id="HFO5820053.1"
                     /translation="MNKHASQPRAIYYVVALQIWEYFSFYGMRALLILYLTNQLKYSD
                     NHAYELFSAYCSLVYVTPILGGFLADKVLGNRMAVMLGALLMAIGHVVLGASEIHPSF
                     LYLSLAIIVCGYGLFKSNVSCLLGELYEPTDPRRDGGFSLMYAAGNVGSIIAPIACGY
                     AQEEYSWAMGFGLAAVGMIAGLVIFLCGNRHFSHTRGVNKKVLRATNFLLPNWGWLLV
                     LLVATPALITVLFWKEWSVYALIVATIIGLGVLAKIYRKAENQKQRKELGLIVTLTFF
                     SMLFWAFAQQGGSSISLYIDRFVNRDIFGYTVPTAMFQSINAFAVMLCGVFLAWVVKE
                     SVAGNRTVRIWGKFALGLGLMSAGFCILTLSARWSAMYGHSSLPLMVLGLAVMGFAEL
                     FIDPVAMSQITRIEIPGVTGVLTGIYMLLSGAIANYLAGVIADQTSQASFDASGAINY
                     SINAYIEVFDQITWGALACVGLVLMIWLYQALKFRNRALALES"
     gene            complement(11881..13299)
                     /gene="phrB"
                     /locus_tag="ACHKBL_003219"
     CDS             complement(11881..13299)
                     /gene="phrB"
                     /locus_tag="ACHKBL_003219"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HFO5820054.1"
                     /translation="MTTHLVWFRQDLRQHDNLALAAACRNSSASVLALYIATPRQWAA
                     HNMSPRQAEFINAQLNGLQIALAEKGIPLLFHEVDDFAASVEIVKQVCAENRVTHLFY
                     NYQYEVNERARDVQAERALRNVVCEGFDDGVILPPGAVMTGNHEMYKVFTPFKNAWLK
                     RLREGMPECVAAPKVRSSGSIDPAPSITLNYPRQPFDTAHFPVEEKAAIAQLRQFCQN
                     GAGEYEQQRDFPAVEGTSRLSASLATGGLSPRQCLHRLLAEQPQALDGGAGSIWLNEL
                     IWREFYRHLITYYPSLCKHRPFIVWTDRVQWQSNSAHLKAWQEGKTGYPIVDAAMRQL
                     NSTGWMHNRLRMITASFLVKDLLIDWREGERYFMSQLIDGDLAANNGGWQWAASTGTD
                     AAPYFRIFNPTTQGEKFDREGEFIRQWLPELRDVPGKVVHEPWKWAQKAGVTLDYPQP
                     IVDHKEARLRTLAAYEEARKGA"
     gene            complement(13296..13805)
                     /gene="ybgA"
                     /locus_tag="ACHKBL_003220"
     CDS             complement(13296..13805)
                     /gene="ybgA"
                     /locus_tag="ACHKBL_003220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HFO5820055.1"
                     /translation="MNQQRFDESTLIRIFALYELHRLKEHGLTRGALNDYHSRYKLVF
                     LAHSQPEYRKLGPFVADIHQWQNLDDYYNQYRQRVVVLLSHPANRRDHTNVLMHVQGY
                     FRPHIDSTERQQLAALIDSYRGGEQPLLAPLMRIKHYMALYPDAWLSGQRYFELWPRV
                     INLRHSGVL"
     gene            complement(13947..14424)
                     /locus_tag="ACHKBL_003221"
                     /pseudo
     CDS             complement(13947..14424)
                     /locus_tag="ACHKBL_003221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421020.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
BASE COUNT         3475 a         3843 c         3502 g         3604 t
ORIGIN      
        1 tttatataac aagcttttta atattgttaa ataaaaccaa atacattcaa ctgtcagtta
       61 atatattctt gcctcgcaac actctatctc attatttaaa ggactcagct atgtttaaag
      121 gacaaaaaac attggccgca ctggccatat ctctgctatt cacagcacct gtttatgcgg
      181 ctgatgaagg atccggtgaa attcacttta aaggtgaagt tattgaagca ccgtgtgaaa
      241 tacatcagga tgatattgat aaagaggttg aactcggtca ggtgaccacc agccacatta
      301 atcagtcgca tcacagcgat gccgttgctg tcgacctgcg cttagtcaac tgcgatctgg
      361 aaaactccaa caacggttcc ggcggcaaga tttctaaagt tgcagtcacc tttgacagct
      421 cagcgaaaac caccggcgca gatccgattc tcaacaacac cagcacgggt gaagcgaccg
      481 gtgtcggcgt gcgtttaatg aataaagatc aaagcaatat cgttttaggc accgctactc
      541 cagatatcga cctggcacca acctccagcg aacaaacgtt aaatttcttt gcgtggatgg
      601 aacaaattga tcaggctacg cccgtaacac caggcgcagt tacggcgaat gcgacctacg
      661 tactggatta taaataagta tcactttggc ggcgtcatcg tcgcctttct tcaaatattt
      721 atcaggtatg gacaccgtga atatttatcg actctctttt atatcctgtc tggtcatggc
      781 gatgccttgc gcattggcgg tcgaattcaa cctgaatgtc ctcgataaat cgatgcgaga
      841 tcgcattgat atttcattat taaagggaaa aggggtcatt gttcccggtg aatattttgt
      901 tagcgttgca gtaaataaca accaaatcag taacggacaa aaaattgact ggaaaaagaa
      961 cggtgaccaa accattccgt gtattaatga tttactggta gataaatttg gtttaaaacc
     1021 tgaagttcgt cagtcgttac cacggttaaa ccagtgcgtc gattttagct cccggcctga
     1081 aattctcttc atttttgatc aggctagcca gcaactgaat atcactattc cacaagcctg
     1141 gctggcgtgg cactcagata actggactcc cccctcaaca tggaaagaag gtgtcgccgg
     1201 cgtcctgatg gactacaacc tgtttgcaag cagctaccgc ccacaggatg gtagcaacag
     1261 cactaacctg aacgcctatg gaacagccgg aattaacgcg ggagcatggc gtttacgcag
     1321 tgattaccaa ttgaatcaga ccgatagcga tgataaccat gaacagtcag gcgaaatatc
     1381 acgcacctat ctttttcgtc cattaccgca attaggttca aaattaaccc ttggtgaaac
     1441 ggatttcagt tccaatattt tcgacggctt ttcttatacc ggcgcggcac tggcaagtga
     1501 cgatcggatg ttaccgtggg agctacgcgg ctacgcccca caaatcagcg gcattgcaca
     1561 aaccaacgcc accgtcaccg tgagtcactc agggcgagtt atttaccaga agaaagtccc
     1621 accaggtccg tttatcattg atgaccttaa tcagtccgtc cagggtacgt tagatgtcaa
     1681 agtgacggaa gaagatggtc gggcgaacaa tttccaggtt tcggcagcat cgacgccttt
     1741 ccttacgcgc caggggcagg ttcgctataa acttgctgcc ggtcagccgc gaacatctat
     1801 gtctcatcaa actgaaaatg aaaccttttt tagcaatgaa gtttcctggg ggatgctgtc
     1861 aaatacctca ctgtacggcg gcctgctgat ttcgggtgac gactaccatt ctgccgcaat
     1921 gggtatcggg caaaatatgc tgtggcttgg tgcattgtcg tttgatgtca cgtgggccag
     1981 tagtcagttt gatactcagc aggacgagcg gggcttaagt taccgcttta attacagcaa
     2041 acaagtggat gccaccgaca gcacgatttc gctcgccgct tatcgtttct ccgatcgtca
     2101 ttttcacagc tacgccaact atctggatca caaatacaac aacagcgatg cgcaggacga
     2161 aaaacagacg atcagcttat ccgtgggcca accgattacc ccactgaacc tcaatctgta
     2221 cgccaacctg ctgcatcaaa cctggtggaa tgcagacgcc tccacgaccg ccaacatcac
     2281 ggcgggtttt aatgttgata ttggtaactg gagagatatc tcgatctcga cgtcattcaa
     2341 tacaacccat tacgaagata aagatcgcga caatcagatt tacctgtcga tttcgctccc
     2401 cttcggtaac ggggggcggg tcggttatga catgcaaaac agtagtcaca gcaccacgca
     2461 ccgcatgtcg tggaatgata cgttagatga acgtaatagc tggggcatgt ctaccggact
     2521 gcaatccgac cgtccggata atggagccca ggtgagcggt aactatcagc acctgagttc
     2581 agcgggtgag tgggatattt ctggtaccta tgccgccaat gattacagtt ccgtcagcag
     2641 cagctggagc ggttctttca cggcaactca atatggtgcg gcgttccatc gtcgcagctc
     2701 caccaatgaa ccacgtctga tggtcagtac cgatggcgtg gcagatattc cggttcaggg
     2761 caatctcgac tacaccaacc attttggcat tgcggtggtg ccgttgattt ccagttatca
     2821 gccttccacc gtggcggtga acatgaatga cttacccgac ggcgtaacag tggcagaaaa
     2881 cgttatcaaa gaaacgtgga ttgaaggcgc gataggttac aaatcgctgg cttcccgttc
     2941 cggtaaagac gttaacgtca tcattcgcaa cgccagcggt cagttccctc ccctcggtgc
     3001 ggatatccgc caggataaca gcggcattag cgtggggatg gttggcgagg aaggacatgc
     3061 ctggttaagc ggagtcgctg aaaatcaaca atttaccgtg atctggggtg atagccagcg
     3121 ttgctcgctc catcttcctg aacatatgga agacaccgca aatcgcctga ttttaccttg
     3181 tcattaatta aggaaatgaa aatgacattt atgaaaggac tacccctttt gttgttggtt
     3241 gccagtctgt gcagccacgc tgcactacaa cccgatcgca ctcgtattgt gtttaacgct
     3301 aatgataaag ctaccagtct gcgcgtggat aatcgtagcg ataaactccc ctacctcgct
     3361 tattcctggc tcgaaaatga aaaaggggag aaaagcgatg atcttctggt tgccctgcca
     3421 ccgatacaac gtctggaacc gaaagcaaca acgcaggtac ggatagtaaa gcaagcctcg
     3481 accacaaaat taccgggcga tcgcgaaacg ctatttttct acaatatgcg tgaaattcct
     3541 ccctccccag aaaaaaacag cggccatgcc gttctgcaag tcgccattca gagtcgtatc
     3601 aaggtattct ggcgaccggc tgcattacgc aagaaagcag gagaaaaggt cgaactgcag
     3661 ttacaggtca gccagcaggg caaccaactg acactgaaaa atcctaccgc gtattatctg
     3721 accattgcct atctggggag aaatgaaaaa ggcgttcttc ccggcttcaa aaccgtgatg
     3781 gttgcgcctt ttagtacagt aattacaaac accggaagtt atagcggcag tcagttttat
     3841 ctcggttata tggatgatta cggcgcgtta cgcatgacga cgctcaactg cagcggacaa
     3901 tgccgtttac aagcagtgga ggcgaagaaa tgagtgctgg caagggagtg ttgctcgtca
     3961 tctgtctggt atttctgcca ttgaagtctg cgctagcgct gaactgctat tttggtactt
     4021 ccggcggaac ggtagaaaaa tcggaagcga ttcaaccctt tgctgtgcca ggtaatgcca
     4081 aacccggcga taagatctgg gaatctgacg atattaaaat tcccgtctat tgtgacaaca
     4141 ataccaacgg caactttgaa agcgaacatg tctacgcatg ggtaaacccc tacccggggg
     4201 tacaggatcg ttattatcaa ctcggcgtga catacaatgg tgtcgattac gatgccagtc
     4261 tggggaaaag ccgcatcgac accaaccagt gtatcgacag taagaacatt aacatttata
     4321 cccctgagca gattatcgcg atgggctggc agaacaaaat atgctcgggt gaccccgcta
     4381 atattcacat gtcgcgaacc tttcttgctc gtatgcggtt atacgttaag attcgcgaaa
     4441 taccgccgca tgattatcaa agttcgctta gcgactacat cgtcgtgcaa tttgatggtg
     4501 ccggtagcgt taacgaagac cctaccgccc aaaacctgaa ataccatatt actggtctgg
     4561 aaaacattcg tgtactggat tgcagcgtca atttttctat ttcaccagaa acacaggtga
     4621 ttgattttgg taaatttaac ctgctggata ttcgccgcca cacaatgtcg aaaacattca
     4681 gtattaagac gacaaaatca caaaatgacc agtgcaccga cggatttaag gtcagctctt
     4741 cgttttatac cgaagaaaca ttgattgaag aggataaagc actgctgatt ggtaatggac
     4801 tgaagctacg tttgctggat gaaaattctt caccctatac tttcaacaaa tacagcgaat
     4861 atgctgattt caccagcgat atgctgatct acgaaaaaac ctatacggct gaactttcgt
     4921 ccattcccgg cacccccatc gaagcgggtc ccttcgatac agtggtgctt tttaagatta
     4981 actataacta atttatcatg atgccctttg cagttagtaa agggcctctc tttcagaaaa
     5041 ttgctgattt tccccttttc cttgattgct gttatttttt cctggcccgg ctttctttat
     5101 gctgtatcga tacaccaaag aaaaaataat gagggaacga aggatgccag ttttgcagtg
     5161 gggaatgtta tgtgtgctct cacttctcct ttcaattggt ttcctcgcgg ttcatctccc
     5221 ggcggcgcta ttacttgggc cgatgatcgc cgggatcatc tttagtatgc gcggaattac
     5281 tctgcaactt ccccgctccg cttttctcgc cgcccaggcc attcttggct gcatgattgc
     5341 gcaaaacctg accggttcga tcctgaccac gctagcggtg aactggccga ttgtgttagc
     5401 tattttactg gtgacgttac tttccagcgc catcgtgggt tggttattgg tgcgctatag
     5461 ctcactgcct ggaaataccg gtgcctgggg ttcttctcct ggcggcgcgg cggcaatggt
     5521 cgctatggcg caagattacg gcgcagatat tcgcctggtg gcgtttatgc aatatctgcg
     5581 agtgctgttc gtcgcagggg ccgctgttct ggtcacccgc atgatgctgg gcgataacgc
     5641 tgaagcggtt aaccagcaga tcgtctggtt tccgccagtg agcattaatc tcctgcttac
     5701 gattttgctc gcggtcgttg ccggtacagt gggatgcttg ctacgtcttc cttccggcac
     5761 gatgctcatc ccgatgctgg cgggcgcagt gctccagtct ggtcagctca tcaccatcga
     5821 actaccggaa tggctgcttg cgatggcgta tatggcaatt ggttggcgga ttggtcttgg
     5881 tttcgataaa caaatcttac tgcgggcatt gcgcccgcta ccgcaaatcc tgctgtcgat
     5941 ttttgctctg ctggctattt gtgcgggtat ggcgtggggg ctgacccggt ttatgcatat
     6001 tgattttatg accgcctacc tcgccaccaa ccccggcggg cttgataccg tggcggtcat
     6061 cgccgcaggg agcaatgccg atatggcgct catcatggcg atgcaaaccc tgcgcctgtt
     6121 cagtattttg ctgacgggac ctgccattgc gcggtttatt tcaacctatg cgccgaagcg
     6181 ttctgcctaa tgctggcagc cagggcacca gtaaaacggg cgagatgaca gcgtggtttt
     6241 ctcaatgatg ccgccacagc gttcgcacag ttcgccatca cgatgaaaaa ccttaaagcg
     6301 aaacagcgcc ccatgatgct tattctcatc cacctgcccc cgcgtagcgt aggaaaatcg
     6361 aggaatatcc agtaacgcgt gtgcgagtgc atccagttgc gccgcattga gatctttcgc
     6421 tttatgattt ccagtcaacc caacctgcca gaggatctcc acccgcaaat aattgccaag
     6481 cccagccaga aacgcctgat cgagcagtaa tccagcaaac tgacggttac gaaagcgcgg
     6541 cgacaataac cgttccttta ccacctccgg cgtcagattc ggatccagca catcggggcc
     6601 gactcgttgt aaaaacggat gcgtgatcag ttgttccggc gtcaacatct caatatccga
     6661 ggcgctataa agcagaatag ttttgtcaac cgtttgcagt tttacccgca atactcgcat
     6721 agtctgcggc tcttcgccgg tatcaaccac gcgccagacg ccgtaaagct gattatggct
     6781 gtagagcgtt aagtcgttgg aaaaatgagt taacaacgcc ttaccacgcg tttccacatg
     6841 ggtaacgtgt tgaccgataa gttgtgattg ataaggtttt aactgcggga aggcaaacca
     6901 gacatcagtt agtggtttgc ctttgatcgc cgcctccagg ttatccgctg cacggcggat
     6961 ctccgggcct tcaggcatgg tgatatcctt ttttcattct ttattaaacg gggttatgct
     7021 gcgacaacaa tccccttttc ggcaaatgta gagcgtagtc ggcgggcgaa tgccagtgcg
     7081 tgctcgccgt cgccatgcag gcagaccgtt tgcgccgtga ccgttgccca ttcgccggtg
     7141 atgcttttga ctctgccgtg ttgcaccatc tccagcgttt gcgccagcgc ctgttcttcg
     7201 ttttcaatca acgcgcctgg ctggcttcgc ggcaccagcg aaccgtctgc ctgataaccg
     7261 cgatcggcaa acacttcctc gcgcgttgtc aggccatatt gcttgcctgc acgaatcagc
     7321 tcgcttcctg ccagcccgac gagaatcaat gccggatcgc aagcgtatac cgctctggcg
     7381 atggcgtctg ccagttgtgc ctctttcgct gcctggttgt ataacatgcc gtgaggtttg
     7441 acatgacgca taacaccgcc ttgcgcacgg gcaatcgttg ccagcgcgcc aatttgatac
     7501 agcgtctggg cgtaaacggt ttctggcggc agctgcatga cgctgcgccc aaaattttcc
     7561 ctgtcgggaa aactcgggtg agcgccaatc gcaacaccat tttttattgc ttcacgcacg
     7621 caagcctgca tggtttgcgc atcgcctgca tgaaatccac aggcaatatt ggcagaggaa
     7681 accagcgtta atagctctgc gtcgctggca tagccttcgc ccagatcggc gttcaggtca
     7741 attttcattg tgcagccgcc acgctaattg ttcgaaataa cgttgctgat cttgccgtgc
     7801 tttcagtgct tcttccagtg aacactggac aaaatgaatc ggctgaccga gcggaatttg
     7861 cgccagatgg tacatatcag cctcaatgat acaggcaata cgcggataac caccggtggt
     7921 ctgtgcgtcg ttcatcaaca caatcggctg cccgttatgc ggcacctgca ccacgcccgg
     7981 taacaaacca tgagataaca gttcgcgatc ggtggtacgt tttaaaattt gcccctgtaa
     8041 gcgatagccc attcggttac tttgcgagct aagctgccag ggcgaacgcc agaatgcatc
     8101 ctgcgaggcg cgatcgaact catgatattc cggccccggc aaggcgcgaa tgcggttgcc
     8161 ccacagcagc tgtttaacgc cctgcgcttc cataaaatca cgcttcgatt tgccaatcgg
     8221 gagtcggtca ccatccttca gtaaacggcc ttccagcccg ccaatcccca ctttgagatc
     8281 ggtgctgcat gaccccatta ccggcggaac atcaatacca cccgcgaccg ccagataact
     8341 gcgcatcccg tgctgcgggc gtttaagcgt taaacgctga cctgctttca tcggcaagcg
     8401 ccagccggtc cagacggcgt tatcatccag ccgcgcttcg caaccggccc ccgtcagagc
     8461 aaaccaccca tcagtttcga actcaaccgt taactgaccg agcgtgatct ccagcgcggg
     8521 ggcattggcg tcattaccca ccagtaggtt agcaatgcgc agcgcaggca tatccagtgc
     8581 gccgcagtgg ctgatacccg actggcgaaa accgtgacga ccgccatcct gcacagtggt
     8641 atacataccc gcacgaataa tcttcagcat actccctcct tctgcggtac aaagcgcacg
     8701 ctgtctcccg gacgtaataa gatgggttcg tcacgcgccg gatcaaacag gctgagtgag
     8761 gtatgaccaa tcaactgcca gccacccggc gttgccaacg gataaatacc agtctgcggc
     8821 ccgccgatcc cgacagaacc tgccggaacg agcaagcgcg gttcagcgcg ccgtggcgtg
     8881 tgtagttgtt ccggcaaact cccgagatac gggaagcccg gttgaaaacc taaaaaccag
     8941 accacgtatt ccacggagga gtgcaattca acaacctgtt tttcgctcaa tccacaatgc
     9001 gccgcgacca ccgccaaatc cggtccggct gcgccaccgt aaaccaccgg aatttcaata
     9061 aagcgagact ccggctccag cgcctcactc tcctcccacc agcgttgcaa acgctctatg
     9121 gcatccagcg ccagcgactc aggattacgc aaaatcactg tgatattgtt catgccagga
     9181 atggcttcaa ccacattcgg catatccacc agacgctgtg ccagtcgcca gatccgtttc
     9241 tggctagcca gcgtcaccgg cggttccagc tccagcacta ccgccgtttc acctatcaga
     9301 taacaacgcg ctcgttgcac tttcgatccc tctgattatt cgttatgcag gattaggaat
     9361 atcaataaag gtcacatcaa gatcggtatt ttcattcagc cactcgctca atgcgcgaat
     9421 accaccgcgt tcagtggcat ggtgacccgc agcataaaaa tgcaatccct gctcgcgggc
     9481 tgaatgaatg gtctgttctg aaacttcgcc agtaataaaa gcatccacgc caaaacgcgc
     9541 ggcgctatcg ataaaactct gcccgccgcc cgtgcaccag gcgacgcgct gaaccacctc
     9601 aggtccggta tcgccacacc ataacggctt acgtcccaga cgcgcttcaa tccaggaagc
     9661 cagctccagt cccggcacag gcatggtcag ttcgccccac ggcaccaacg gctcaatttc
     9721 gcccatgacc gtgatcccca gtaacgccgc cagttgtgcg ttattaccca gctccggatg
     9781 cgcgtccagg ggcagatgcc agccatacag gttgatatca ttcgccagca acgtttttaa
     9841 acggttacgc ttcattccgc gaatgaccgg agattcacct ttccagaagt agccatgatg
     9901 cacaatgacc gcatccgcgc ccagacgcac cgcttcatcg agcaaagcct ggctggcggt
     9961 gacaccggta acaatttttt gcaccgtctc tttgccttcc acctgcaaac cattcggcgc
    10021 gtaatcacta atcgccgcgc tgttcagttt ttcgttaatc agttgttcca gttcggtgtt
    10081 tttcatcttc gcgccttccg tattctgata gctacaccgt taacatagcg ttacctcgcc
    10141 tgttcgtcga tagccaatct tttgacatat taagaaacaa acccgacgcc aaaagacgca
    10201 aattgtttat atcatcacgc ttcagctaat gtatatttat gcaatcagac agcatattgc
    10261 ataaataatc agatctgtat caaagtttta actttaagga aacacaagca cgccgcgcaa
    10321 ctattacgtg acgttgcagt caattgtgga attattatga ataaacacgc atcacaaccg
    10381 cgcgctattt actatgtcgt tgcgctgcaa atctgggaat attttagctt ttacggcatg
    10441 cgtgccctgc tgatcctcta tctcaccaat caactaaaat atagcgataa tcacgcctac
    10501 gagttattta gcgcctactg ttcgctggta tatgtcacgc caatcctcgg tggctttttg
    10561 gcggataaag ttctcggcaa tcgcatggcg gtgatgctgg gtgcgttgtt gatggcgatc
    10621 ggtcatgtgg tgctgggtgc cagtgagatc catccgtcat tcctctatct gtccctggcg
    10681 attatcgttt gtggctatgg tctgtttaaa tccaacgtca gttgcctgct cggcgagctg
    10741 tatgagccaa ccgatccgcg tcgtgatggc ggtttctcgc tgatgtatgc ggcgggtaac
    10801 gtggggtcta ttatcgcacc tatcgcctgt ggctacgccc aggaagagta cagttgggcg
    10861 atgggctttg gcctggcggc ggttggcatg atcgcgggtc tggtcatttt cttatgtggc
    10921 aatcgtcatt tctctcatac ccgcggcgtt aacaaaaaag tactgcgtgc gacaaacttt
    10981 ctcctgccca actggggatg gctgctggtt ctgctggtgg caacacctgc gctgatcacc
    11041 gtactgttct ggaaagagtg gtcggtatac gccttgattg tcgcgactat tattggcctg
    11101 ggtgtattgg caaaaattta tcgcaaagca gaaaaccaga aacagcgtaa ggagctgggg
    11161 ctgattgtta ccctcacctt cttcagcatg ttgttctggg cattcgcaca acaggggggt
    11221 agctcgatta gcctttatat cgaccgcttc gttaaccgcg atatatttgg ttataccgtt
    11281 ccgactgcta tgttccagtc gattaatgcc ttcgcagtta tgctgtgcgg tgtgttcctg
    11341 gcgtgggtgg ttaaagaaag cgtcgcaggt aatcgtaccg tgcgcatctg ggggaaattt
    11401 gctcttggtc tcggcctgat gagcgccgga ttctgcattc tgaccttaag cgcccgctgg
    11461 tctgcaatgt atggtcactc ttctctgcca ctgatggtgt taggcctggc ggtgatgggg
    11521 tttgcggaac tgtttatcga cccggttgct atgtcgcaga ttacgcgcat tgaaatcccc
    11581 ggcgtgaccg gcgtattaac cggcatttat atgctgcttt ccggcgcgat tgcgaactat
    11641 ctggccggcg tgattgccga tcagacatcg caggcttcgt ttgatgcttc cggagcgatc
    11701 aactactcca tcaatgcata tattgaagta tttgatcaaa ttacctgggg cgcactggcg
    11761 tgtgtaggac tggtgctgat gatttggctg tatcaggcgc ttaagttcag aaaccgcgcg
    11821 ctggcgctgg aatcttaaca ggccagcacc gatgcctgat aagacgctcg ctgcgtttta
    11881 tcaggctcct ttcctcgctt cttcgtacgc tgccagcgtt cgcagccttg cctctttgtg
    11941 atcaactatc ggttgcggat aatccagcgt cacacctgct ttctgcgccc acttccacgg
    12001 ctcatgcacc actttccctg gcacatcgcg cagttccggt agccactggc ggataaactc
    12061 gccctcacgg tcaaatttct cgccctgggt tgtcgggtta aaaatacgaa aatacggcgc
    12121 tgcatcggtt ccggttgaag cggcccactg ccagccaccg ttattggctg ccaaatcacc
    12181 atcaatcagc tgcgacatga aatatcgctc accttcgcgc cagtcgatca ataaatcttt
    12241 caccaaaaaa ctggctgtaa tcatccgtag ccggttatgc atccagccgg tgctgttaag
    12301 ctgacgcatg gcagcatcaa caatcgggta tcccgttttg ccttcctgcc aggcctttaa
    12361 atgcgcggaa ttgctctgcc actgcacgcg gtcggtccag acaataaacg gacgatgttt
    12421 acacagcgag gggtaatacg ttatcagatg acggtaaaac tcgcgccaga tcagctcatt
    12481 aagccagata ctaccggccc caccgtccag cgcctgcggc tgttccgcca acaggcgatg
    12541 caaacactgg cgaggcgata acccgcccgt tgccaggctg gcggacaaac ggctggtgcc
    12601 ttctaccgca gggaaatctc gctgttgctc atactctccg gcaccgttct ggcaaaactg
    12661 gcgtaattgc gcaatcgccg ctttctcttc caccgggaaa tgcgcagtat cgaaaggctg
    12721 acgaggataa ttcagcgtaa tggatggcgc gggatctatc gatccgctac tacgtacttt
    12781 tggcgcagcg acgcactccg gcatcccttc tcgcagccgt ttaagccagg cgtttttaaa
    12841 tggcgtaaag actttgtaca tctcatgatt gccagtcatc accgcccccg gcggcaggat
    12901 cacgccgtcg tcaaatcctt cacacaccac gttacgcagc gccctctcag cctgcacatc
    12961 ccgcgcccgc tcattcacct catactgata gttataaaac agatgggtaa cacgattttc
    13021 cgcgcacacc tgtttaacta tttcgacgct ggcggcaaag tcatccactt catgaaacag
    13081 caaaggaatg cctttttccg ccagcgcgat ttgtagcccg ttcagttgtg cattgataaa
    13141 ttcagcctga cgcggcgaca tattatgcgc cgcccactgg cgtggtgtag cgatatacaa
    13201 cgccagcacg cttgcagacg aattgcggca ggcggcagcc agcgcgagat tatcatgctg
    13261 acgtaaatcc tggcgaaacc agaccagatg ggtagtcata aaactcctga atggcgcaag
    13321 ttaatcacac gcggccaaag ttcgaaataa cgctgccctg aaagccaggc gtcaggataa
    13381 agcgccatat agtgtttgat acgcatcagc ggcgcaagaa gcggttgctc gccaccgcga
    13441 taactgtcga taagcgccgc cagctgctgg cgttctgtgg aatcaatatg agggcgaaaa
    13501 taaccctgaa cgtgcatcaa aacattggtg tggtcacgtc ggttggcggg gtgagaaagc
    13561 aaaacaacta cgcgttggcg gtactggttg taatagtcat ccagattttg ccactggtga
    13621 atatcagcca cgaacgggcc aagtttgcgg tactcgggct gggaatgggc cagaaagacg
    13681 agtttatagc ggctgtgata atcgttgagt gccccgcgcg ttaagccatg ttctttcagt
    13741 cgatgtaact cataaagcgc gaagatacgg attagagtgc tttcatcaaa tcgttgctga
    13801 ttcattactg ctcctgagat attaacagga gtaagtgtag acgttggcaa atgatgtttg
    13861 tgaagttaat cctgttgtag cagggcaaaa ttacgaaagc acgctccccg caaggactga
    13921 cgccagatat tttctgtcca tggctgccct ttccattcaa acgtgaaatc aataagttca
    13981 tcagggacat cgcaaacaat atgaagacgg atttcttctc tgccgtgact cttttcactc
    14041 attgcgtaac tgccatgctc tggattattt aattctttca gcggaaattt ttcctcgaag
    14101 gctttattta gccgcccctg gtttcctttt acagcgaata aataatcacc gccctgtttt
    14161 tgtatcttct ctgcaatatc tttctggcaa cccatcgcat cagttgtgat gatttttcct
    14221 ttaatattca gcatgttaag aagttccggg atagctgtga tctcattgga tttctcatcc
    14281 gtcttgatct gtccgatgac cagactgtgc attgttgaga acgcactaat gacatgaatc
    14341 gctcccttgc ggcgactctt gtcataagag tgccggagcg tttttccatc aattgcaatg
    14401 acgtctttat catctgaaga atgg
//
LOCUS       DAZFLD010000128        14122 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.129, whole genome shotgun sequence.
ACCESSION   DAZFLD010000128 DAZFLD010000000
VERSION     DAZFLD010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14122)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14122)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14122
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.129"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            134..3877
                     /gene="narG"
                     /locus_tag="ACHKBL_003222"
     CDS             134..3877
                     /gene="narG"
                     /locus_tag="ACHKBL_003222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HFO5820056.1"
                     /translation="MSKFLDRFRYFKQKGETFADGHGQLLNTNRDWEDGYRQRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWETQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPMMRKRLMKMWREAKALHSDPVEAWASIIEDADKAKSFKQARGRGGFVR
                     SSWQEVNELIAASNVYTIKNYGPDRVAGFSPIPAMSMVSYASGARYLSLLGGTCLSFY
                     DWYCDLPPASPQTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTVAVTPDYAEIAKLCDLWLAPKQGTDAAMALAMGHVMLREFHLDNPSQYFTDYVRRY
                     TDMPMLVMLEERDGYYAAGRMLRAADLVDALGQENNPEWKTVAFNTNGEMVAPNGSIG
                     FRWGEKGKWNLEQRDGKTGEETELQLSLMGSQDEIAEVGFPYFGGDGTEHFNKVELEN
                     VLLHKLPVKRLQLADGSTALVTTVYDLTLANYGLERGLNDVNCATSYDDVKAYTPAWA
                     EQITGVSRSQIIRIAREFADNADKTHGRSMIIVGAGLNHWYHLDMNYRGLINMLIFCG
                     CVGQSGGGWAHYVGQEKLRPQTGWQPLAFALDWQRPARHMNSTSYFYNHSSQWRYETV
                     TAEELLSPMADKSRYTGHLIDFNVRAERMGWLPSAPQLGTNPLTIAREAEKAGMNPVD
                     YTVKSLKEGSIRFAAEQPENGKNHPRNLFIWRSNLLGSSGKGHEFMLKYLLGTEHGIQ
                     GKDLGQQGGVKPEEVDWQDNGLEGKLDLVVTLDFRLSSTCLYSDIILPTATWYEKDDM
                     NTSDMHPFIHPLSAAVDPAWEAKSDWEIYKAIAKKFSEVCVGHLGKETDIVTLPIQHD
                     SAAELAQALDVKDWKKGECDLIPGKTAPHIMVVERDYPATYERFTSIGPLMEKIGNGG
                     KGIAWNTQSEMDLLRKLNYTKAEGPAKGQPMLNTAIDAAEMILTLAPETNGQVAVKAW
                     AALSEFTGRDHTHLALNKEDEKIRFRDIQAQPRKIISSPTWSGLEDEHVSYNAGYTNV
                     HELIPWRTLSGRQQLYQDHQWMRDFGESLLVYRPPIDTRSVKEVMGQKSNGNPEKALN
                     FLTPHQKWGIHSTYSDNLLMLTLGRGGPVVWLSEADAKELGIADNDWIEVFNSNGALT
                     ARAVVSQRVPAGMTMMYHAQERIVNLPGSEITSQRGGIHNSVTRITPKPTHMIGGYAH
                     LAYGFNYYGTVGSNRDEFVVVRKMKNIDWLDGEGNDQVQESVK"
     gene            3874..5412
                     /gene="narH"
                     /locus_tag="ACHKBL_003223"
     CDS             3874..5412
                     /gene="narH"
                     /locus_tag="ACHKBL_003223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HFO5820057.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNV
                     ETKPGQGFPTDWENQEKYKGGWIRKINGKLQPRMGNRAMLLGKIFANPHLPGIDDYYE
                     PFDFDYQNLHTAPEGSKSQPIARPRSLITGERMAKIEKGPNWEDDLGGEFDKLAKDKN
                     FDNIQKAMYSQFENTFMMYLPRLCEHCLNPACVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRMCITGCPYKKIYFNWKSGKSEKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYD
                     ADAIERAASTENEKDLYQRQLDVFLDPNDPKVIEQAIKDGIPLSVIEAAQQSPVYKMA
                     MEWKLALPLHPEYRTLPMVWYVPPLSPIQSAADAGELGSNGILPDVESLRIPVQYLAN
                     LLTAGDTKPVLRALKRMLAMRHYKRAETVDGKVDTRALEEVGLTEAQAQEMYRYLAIA
                     NYEDRFVVPSSHRELAREAFPEKNGCGFTFGDGCHGSDTKFNLFNSRRIDAIDVTSKT
                     EPHP"
     gene            5409..6119
                     /gene="narJ"
                     /locus_tag="ACHKBL_003224"
     CDS             5409..6119
                     /gene="narJ"
                     /locus_tag="ACHKBL_003224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HFO5820058.1"
                     /translation="MIELVIVSRLLEYPDAALWQHQQEMFEAIAASKNLPKEDAHALG
                     IFLRDLTTMDPLDAQAQYSELFDRGRATSLLLFEHVHGESRDRGQAMVDLLAQYEQHG
                     LQLNSRELPDHLPLYLEYLAQLPQSEAVEGLKDIAPILALLSARLQQRESRYAVLFDL
                     LLKLANTAIDSDKVAEKIADEARDDTPQALDAVWEEEQVKFFAEKGCGDSAITAHQRR
                     FAGAVAPQYLNITTGGQH"
     gene            6119..6796
                     /gene="narI"
                     /locus_tag="ACHKBL_003225"
     CDS             6119..6796
                     /gene="narI"
                     /locus_tag="ACHKBL_003225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HFO5820059.1"
                     /translation="MQFLNMFFFDIYPYIAGAVFLIGSWLRYDYGQYTWRAASSQMLD
                     RKGMNLASNLFHIGILGIFVGHFFGMLTPHWMYEAWLPIEVKQKMAMFAGGASGVLCL
                     IGGVLLLKRRLFSPRVRATTTGADILILSLLVIQCALGLLTIPFSAQHMDGSEMMKLV
                     GWAQSVVTFHGGASQHLDGVAFIFRLHLVLGMTLFLLFPFSRLVHIWSVPVEYLTRKY
                     QLVRARH"
     gene            complement(6981..7112)
                     /locus_tag="ACHKBL_003226"
     ncRNA           complement(6981..7112)
                     /ncRNA_class="other"
                     /locus_tag="ACHKBL_003226"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(7159..7243)
                     /locus_tag="ACHKBL_003227"
     tRNA            complement(7159..7243)
                     /locus_tag="ACHKBL_003227"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7207..7209),aa:Tyr,seq:gta)
     gene            complement(7403..8245)
                     /gene="purU"
                     /locus_tag="ACHKBL_003228"
     CDS             complement(7403..8245)
                     /gene="purU"
                     /locus_tag="ACHKBL_003228"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008864 - formyltetrahydrofolate
                     deformylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HFO5820060.1"
                     /translation="MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHR
                     TGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLL
                     MKANYGGLDVEIAAVIGNHDTLRSLVERFDIPFELVSHEGLSRNEHDQKMADAIDAYQ
                     PDYVVLAKYMRVLTPEFVARFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAH
                     YVNDNLDEGPIIMQDVIHVDHTYTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNR
                     TIIL"
     gene            complement(8295..8753)
                     /gene="ychJ"
                     /locus_tag="ACHKBL_003229"
     CDS             complement(8295..8753)
                     /gene="ychJ"
                     /locus_tag="ACHKBL_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HFO5820061.1"
                     /translation="MSQLCPCGSAVEYSLCCHPYVSGEKVAPDPEHLMRSRYCAFVMK
                     DADYLIKTWHPSCGAATLRAELIAGFAHTEWLGLTVFEHCWQDVDNIGFVSFVARFTE
                     GSKTGAIIERSRFLKENGQWYYIDGTRPQFGRNDPCPCGSGKKFKKCCGQ"
     gene            8866..9771
                     /gene="rssA"
                     /locus_tag="ACHKBL_003230"
     CDS             8866..9771
                     /gene="rssA"
                     /locus_tag="ACHKBL_003230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004622 - lysophospholipase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HFO5820062.1"
                     /translation="MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGS
                     LVGAAYACDRLSALEDWVTSFSYWDVLRLMDLSWQRGGLLRGERVFNQYREIMPETEI
                     ENCSRRFAAVATNLSTGRELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVN
                     PIPISLTRALGADIVIAVDLQHDAHLMQQDLLSFNVSEENSENGDSLPWHARLKERLG
                     SITTRRAVTAPTATEIMTTSIQVLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAH
                     AAIAAGQLAVEKKMDELLPLVRTNI"
     gene            9863..10876
                     /gene="rssB"
                     /locus_tag="ACHKBL_003231"
     CDS             9863..10876
                     /gene="rssB"
                     /locus_tag="ACHKBL_003231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RssB"
                     /protein_id="HFO5820063.1"
                     /translation="MTQPLVGKQILIVEDEQVFRSLLDSWFSSLGATTVLAADGVDAL
                     ELLGGFTPDLMICDIAMPRMNGLKLLEHIRNRGDQTPVLVISATENMADIAKALRLGV
                     EDVLLKPVKDLNRLREMVFACLYPSMFNSRVEEEERLFRDWDAMVDNPAAAAKLLQEL
                     QPPVQQVISHCRVNYRQLVAADKPGLVLDIAALSENDLAFYCLDVTRAGHNGVLAALL
                     LRALFNGLLQEQLAHQNQRLPELGALLKQVNHLLRQANLPGQFPLLVGYYHRELKNLI
                     LVSAGLNATLNTGEHQVQISNGVPLGTLGNAYLNQLSQRCDAWQCQIWGTGGRLRLML
                     SAE"
     gene            11078..11986
                     /gene="galU"
                     /locus_tag="ACHKBL_003232"
     CDS             11078..11986
                     /gene="galU"
                     /locus_tag="ACHKBL_003232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFO5820064.1"
                     /translation="MAAINTKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQY
                     VVNECIAAGITEIVLVTHSSKNSIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHV
                     TIMQVRQGLAKGLGHAVLCAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMIRRFD
                     ETGHSQIMVEPVADVTAYGVVDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRY
                     VLSADIWPLLAKTPPGAGDEIQLTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAF
                     VEYGIRHNTLGTEFKAWLEEEMGIKK"
     gene            complement(12131..12544)
                     /gene="hns"
                     /locus_tag="ACHKBL_003233"
     CDS             complement(12131..12544)
                     /gene="hns"
                     /locus_tag="ACHKBL_003233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0071824 - protein-DNA complex organization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histone-like nucleoid-structuring protein H-NS"
                     /protein_id="HFO5820065.1"
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEE
                     SAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLAAVKSGTKAKRAQRPAKYSYVDE
                     NGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ"
     gene            13148..13765
                     /gene="tdk"
                     /locus_tag="ACHKBL_003234"
     CDS             13148..13765
                     /gene="tdk"
                     /locus_tag="ACHKBL_003234"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HFO5820066.1"
                     /translation="MAQLYFYYSAMNAGKSTALLQSSYNYQERGMRTVVYTAEIDDRF
                     GAGKVSSRIGLSSPAKLFNQNSSLFDEIRAEHEQQAIHCVLVDECQFLTRQQVYELSE
                     VVDQLDIPVLCYGLRTDFRGELFIGSQYLLAWSDKLVELKTICFCGRKASMVLRLDQA
                     GRPYNEGEQVVIGGNERYVSVCRKHYKEALEVGSLTAIQERHRHD"
     gene            complement(14048..>14122)
                     /locus_tag="ACHKBL_003235"
     CDS             complement(14048..>14122)
                     /locus_tag="ACHKBL_003235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5820067.1"
                     /translation="AFPRVVKERPWKYPTAPKKSQSVA"
BASE COUNT         3486 a         3388 c         3758 g         3490 t
ORIGIN      
        1 tttactttat ttttcatccc catcactctt gatcgttatc aattcccacg ctgtttcaga
       61 gcgttacctt gcccttaaac attagcaatg tcgatttatc agagagccga caggctccca
      121 caggagaaaa ccgatgagta aattcctgga ccggtttcgc tacttcaaac agaagggcga
      181 aacctttgcc gatgggcatg gccagcttct caataccaac cgtgactggg aggatggata
      241 tcgccagcgt tggcagcatg acaaaatcgt ccgctctacc catggggtaa actgcaccgg
      301 ctcctgcagc tggaaaatct acgttaaaaa cggtctggtc acctgggaaa cccagcagac
      361 tgactatccg cgtactcgcc cggatctgcc aaaccatgaa cctcgcggct gcccgcgcgg
      421 tgccagctac tcctggtatc tttacagcgc caaccgtctg aaatacccga tgatgcgcaa
      481 acgcctgatg aaaatgtggc gtgaagcgaa ggcgctgcat agcgatccgg ttgaggcatg
      541 ggcttctatc attgaagacg ccgataaagc gaaaagcttt aagcaggcgc gtggacgcgg
      601 tggttttgtt cgttcttcct ggcaggaagt gaacgaactg atcgccgcat ctaacgttta
      661 caccatcaaa aactacggcc cggaccgtgt cgctggcttc tcgccaattc cggcaatgtc
      721 gatggtttct tacgcgtcgg gtgcgcgtta tctctcgctg cttggcggta cttgcttaag
      781 cttctacgac tggtactgcg atctgcctcc tgcgtctcca caaacctggg gcgagcaaac
      841 tgacgtaccg gaatctgctg actggtacaa ctccagctac atcatcgcct gggggtcaaa
      901 cgtgccgcag acgcgtactc cggatgctca cttctttact gaagtgcgtt acaaaggcac
      961 caaaaccgtt gccgtcacgc cggactacgc tgaaatcgcc aaactgtgcg atctgtggct
     1021 ggcaccgaag cagggcaccg acgcagcaat ggcgctggcg atgggtcacg tgatgctgcg
     1081 tgaattccac ctcgacaacc caagccagta tttcaccgac tatgtgcgtc gttataccga
     1141 catgccaatg ctggtgatgc tggaagagcg cgacggttac tacgctgcgg gtcgtatgct
     1201 gcgcgctgct gatctggtgg atgcgctggg ccaggaaaac aatccggaat ggaaaactgt
     1261 cgcctttaat accaatggag aaatggttgc gccgaacggt tctattggct tccgctgggg
     1321 cgagaagggc aaatggaatc ttgagcagcg cgacggcaaa actggcgaag aaaccgagct
     1381 gcaactgagc ctgatgggta gccaggatga gatcgctgaa gtgggcttcc catactttgg
     1441 tggcgacggc acggaacact tcaacaaagt ggaactggaa aacgtgctgc tgcacaagct
     1501 gccggtgaaa cgcctgcaac tggctgatgg cagcactgct ctggtgacca ccgtttatga
     1561 tctgacgttg gcaaactacg gtctggaacg tggcctgaac gacgttaact gcgcaaccag
     1621 ctatgacgac gtgaaagcct acacccctgc atgggccgag cagatcactg gcgtttctcg
     1681 cagccagatt atccgcattg cccgtgaatt tgccgataac gctgacaaga cgcacggtcg
     1741 ttcgatgatt atcgtcggtg cggggctgaa ccactggtat cacctcgata tgaactatcg
     1801 tggtctgatc aacatgctga ttttctgcgg ctgtgtaggt cagagcggtg gtggctgggc
     1861 gcactatgta ggtcaggaaa aactgcgtcc gcaaaccggc tggcagccgc tggcgtttgc
     1921 tcttgactgg cagcgtccgg cgcgtcacat gaacagcacc tcttatttct ataaccactc
     1981 cagccagtgg cgttatgaaa ccgtgaccgc agaagagttg ttgtcgccga tggcggataa
     2041 atcccgttat accggacatc tgatcgactt taacgtccgt gcagaacgta tgggctggct
     2101 gccgtctgcg ccgcagttag gcactaaccc gctgactatc gctcgcgaag cagaaaaagc
     2161 cgggatgaat ccggtggact atacggtgaa atccctgaaa gagggttcca tccgttttgc
     2221 ggcagaacaa ccagaaaacg gtaaaaacca cccgcgtaac ctgttcatct ggcgttctaa
     2281 cctgctcggt tcttccggta aaggtcatga gtttatgctc aagtacctgc tggggacgga
     2341 gcacggtatc cagggtaaag acctgggtca gcagggcggc gtgaagccgg aagaagtgga
     2401 ctggcaggac aacggtctgg aaggcaagtt ggatctggtg gtgacgctgg acttccgtct
     2461 gtcgagcacc tgtctctatt ccgacatcat tttgccgacg gcgacctggt acgaaaaaga
     2521 cgacatgaat acttcggata tgcatccgtt tattcacccg ctgtctgctg cggtcgatcc
     2581 ggcctgggaa gcgaaaagcg actgggaaat ctacaaagcc atcgcgaaga aattctccga
     2641 agtgtgcgtt ggtcatctgg gcaaagaaac cgacatcgtc acgctgccta tccagcacga
     2701 ctctgccgct gaactggcgc aagcgctgga tgtgaaagac tggaaaaaag gcgaatgtga
     2761 cctgatccca ggtaaaaccg caccgcacat tatggttgta gagcgcgatt atccggcaac
     2821 ttacgaacgc tttacctcta tcggcccgct gatggagaaa atcggtaacg gcggtaaagg
     2881 gattgcctgg aacacccaga gcgagatgga tctgctgcgt aagctcaact acaccaaagc
     2941 ggaaggtccg gcgaaaggcc agccgatgct caacaccgca attgatgcag cagagatgat
     3001 cctgacgctg gcaccagaaa ccaacggcca ggtggccgtg aaagcctggg cggcattgag
     3061 tgagtttacc ggtcgtgacc atacgcatct ggcgctgaat aaagaagacg agaagatccg
     3121 cttccgtgac attcaggcgc agccgcgcaa aatcatctcc agcccgacct ggtctggcct
     3181 tgaagatgaa catgtttctt acaacgccgg ttacaccaac gttcacgagc tgatcccgtg
     3241 gcgtacgctc tctggccgtc agcagctgta tcaggatcat cagtggatgc gtgatttcgg
     3301 tgaaagcctg ctggtttatc gtccgccgat cgacacccgt tcggtgaaag aagtgatggg
     3361 ccagaaatct aacggtaatc cggaaaaagc gctcaacttc ctgacaccgc accagaagtg
     3421 gggtatccac tccacctaca gcgacaacct gctgatgctg actttaggtc gcggtggtcc
     3481 ggtggtctgg ttgagtgaag ccgatgccaa agagctgggt atcgccgaca acgactggat
     3541 tgaagtcttc aacagcaacg gtgcattgac tgcccgtgcg gttgtcagcc agcgtgttcc
     3601 ggcagggatg accatgatgt accacgcgca ggaacgtatc gttaacctgc cgggttcaga
     3661 aatcaccagc cagcgcggtg gtattcataa ctcggtcacc cgtatcacgc cgaaaccgac
     3721 gcatatgatc ggcggctatg cccatctggc atacggcttt aactactacg gcactgtagg
     3781 ttctaaccgc gatgaatttg ttgtagtgcg taagatgaag aacattgact ggttagatgg
     3841 cgaaggcaat gaccaggtac aggagagcgt aaaatgaaaa ttcgttcaca agtcggcatg
     3901 gtgctgaatc tggataagtg catcggttgc cacacctgtt cagtgacctg taaaaacgtc
     3961 tggaccagcc gtgaaggcgt ggaatacgcg tggttcaaca acgtggaaac caagccgggc
     4021 cagggcttcc cgacagactg ggaaaaccag gaaaaataca aaggcggctg gatccgtaaa
     4081 atcaacggca aactgcaacc gcgcatgggt aaccgcgcca tgctgctggg taaaattttc
     4141 gctaacccgc atctgccggg gatcgacgat tattacgagc cgttcgattt tgactatcag
     4201 aacctgcata ccgcgccgga aggcagcaaa tcgcagccga ttgcccgtcc acgttcgctg
     4261 attaccggcg aacggatggc gaaaatcgaa aaagggccaa actgggaaga tgacctgggc
     4321 ggcgagtttg acaaactggc gaaagacaag aacttcgaca acatccagaa ggcgatgtat
     4381 agccagttcg aaaacacctt catgatgtat ttgccacgcc tgtgcgaaca ctgcctgaac
     4441 ccggcatgtg tggcgacctg cccgagcggt gcgatttaca agcgtgaaga agatggcatc
     4501 gtcctgatcg accaggacaa atgccgtggc tggcgtatgt gcatcaccgg atgcccgtac
     4561 aaaaaaatct acttcaactg gaagagcggt aagtctgaga agtgcatctt ctgctatccg
     4621 cgtattgaag ccggtcagcc gacggtgtgc tctgaaacct gtgtcggtcg tatccgttat
     4681 cttggcgtgc tgctgtacga tgccgacgcg attgaacgtg cagccagcac cgagaacgag
     4741 aaagatctct accagcgtca gctggacgtg ttcctcgatc cgaacgatcc gaaggtcatc
     4801 gagcaggcga ttaaagacgg tattccgctg agcgttattg aagccgcaca gcagtcgccg
     4861 gtttataaaa tggcgatgga atggaaactg gcgctgccgc tgcatccgga atatcgcacg
     4921 ctgccgatgg tctggtatgt gccacctctg tcgccgattc agtctgcggc agatgcgggt
     4981 gagctgggca gcaatggcat tctgccagac gtcgaaagcc tgcgtattcc ggtacagtat
     5041 ctggcgaatc tgctgaccgc aggtgatacc aaaccggtac tgcgcgcgct gaaacgtatg
     5101 ctggcgatgc gtcattataa acgtgctgaa accgttgacg gtaaagttga tacccgtgcg
     5161 ctggaagagg tcggtctgac cgaagcccag gcacaggaga tgtaccgtta tctggcgatt
     5221 gctaactacg aagatcgctt tgtggtgccg agcagtcatc gtgaactggc gcgggaagcc
     5281 ttcccggaga aaaatggctg cggctttacc tttggtgatg gctgccacgg ctcagatacc
     5341 aaattcaatc tgtttaacag ccgtcgtatc gatgccatcg acgtgaccag taaaacggag
     5401 ccgcacccat gatcgaactc gtgattgtat cgcgtctcct tgaatatccg gatgctgcct
     5461 tatggcagca tcaacaagag atgtttgagg cgattgccgc gtcgaaaaat ctgccaaaag
     5521 aggatgccca tgcgctgggc attttcctgc gcgatttaac gacgatggat ccgctcgatg
     5581 cccaggcgca gtacagcgaa ctgttcgacc gtggccgcgc cacgtcactg ttgctatttg
     5641 aacatgttca cggtgaatcc cgtgaccgcg gccaggcgat ggtggatctg ctggcgcagt
     5701 acgagcagca cggcttgcag ttaaacagcc gcgaattgcc cgaccatctg ccgctgtatc
     5761 tggagtacct ggcgcagctg ccgcaaagcg aagccgtgga gggtttgaaa gatattgcgc
     5821 cgattctggc attgctgagc gcgcgtctgc aacagcgtga aagccgttat gccgtgctgt
     5881 ttgatctgct gctgaaactg gcgaataccg ccatcgacag cgacaaagtg gcggaaaaaa
     5941 ttgccgatga agcgcgcgac gatacgccgc aggcgctgga tgcagtctgg gaagaagagc
     6001 aggttaaatt ctttgctgaa aaaggctgcg gtgattcagc aatcactgct catcagcgtc
     6061 gctttgccgg tgccgtcgcg ccgcaatatc tgaatatcac caccggagga cagcactaat
     6121 gcaattcctg aatatgttct tctttgatat ctacccgtac atagccgggg cggtcttcct
     6181 gattggtagc tggctgcgtt atgactacgg gcagtacacc tggcgcgcgg cgtccagcca
     6241 gatgctggat cgcaaaggga tgaacctggc gtcgaacctg ttccatatcg gtattctggg
     6301 gatttttgtt ggtcacttct tcggtatgct gacgccgcac tggatgtatg aagcctggct
     6361 gccgattgaa gtgaaacaga aaatggcgat gtttgctggt ggtgccagcg gtgtgctgtg
     6421 tctgattggc ggcgtgctgt tgctgaagcg tcgtctgttc agcccacgcg tgcgtgcaac
     6481 cactaccgga gcggatatcc tgatcctgtc gctgctcgtt atccagtgcg cgctgggcct
     6541 gttgaccatt ccgttctccg cccaacatat ggacggtagc gagatgatga aactggttgg
     6601 ctgggcgcag tcggtagtga ccttccacgg tggcgcttct caacaccttg atggtgtggc
     6661 gtttatcttc cgtctgcacc tggtgttggg gatgacgtta ttcctgctgt tcccgttctc
     6721 gcgtctggtg cacatctgga gcgtaccggt ggagtatctg acacgtaagt atcagctggt
     6781 gcgcgctcgt cactaagcga attttagttt gcatagaccc cgcttcggcg gggttttttt
     6841 atgggcacgg tacggggtga gttgtcggat gcgcttcgct tatccgacct acaggggagg
     6901 atattgtagg tccggtaaac gcggtgccat tgggcagaat tagctcggag taacaggttt
     6961 tgagtattta caggaagtga tggtggtgga ggaaggatta ctcagcgctg cgcgcttcgc
     7021 ccttcgggtc gttgcctgcg gccacgctct ctcgctggcg ctcgagtcga accctggtcg
     7081 aagcttctca tccttccccg cttgggcaga atatttaatc gcggattcgt ttgagagttc
     7141 acggactttt gaaagtgatg gtggtggggg aaggattcga accttcgaag tcgatgacgg
     7201 cagatttaca gtctgctccc tttggccgct cgggaacccc accacggggt aatgcttttt
     7261 actagcctgc tcccttatcg ggaagcgggg cgcatcatat caaatgacgc gccgctgtaa
     7321 agtgttacgt tgagaaaaat gaactggttg cgtaattttc acccgtaacg gattaaaggt
     7381 aaccagttat ttttgctggc gattaaagaa taatcgtccg attaccgtaa acaaagacgc
     7441 gctgcgccag tactttgtag agtgcgcgac ttaagacgtt tttctcgacg tcacgacctg
     7501 cgcgcatcat atcttcagct gtgtaggtat gatcgacatg aataacgtcc tgcatgatga
     7561 ttggaccttc gtccagattg tcattcacat agtgagcggt agcgccaata atcttcacgc
     7621 cacgttcata ggcctggtga taaggacgtg cgccgataaa cgctggcagg aatgaatggt
     7681 gaatattgat gatcttattc gggaagcgtg ccacaaattc tggcgttaat acccgcatat
     7741 acttcgccag caccacgtag tcaggctgat aagcatcaat ggcatccgcc atcttttgat
     7801 cgtgctcgtt gcggcttaac ccttcatggc ttaccagctc aaacggaata tcaaaacgct
     7861 caaccagaga acgtaaagta tcgtggttac caataactgc cgcgatttcg acatccaggc
     7921 cgccgtaatt ggctttcatc aacaaatcgc caaggcaatg cgcctcttta gtgaccagaa
     7981 tcactatccg gcgacgaccg gcaggattca gctcacgtac ggagccttct ggcaatgcgc
     8041 tgtcgagatc cgccagcagg gtggaatcat taaaaattcc ttccagttcc gtgcgcataa
     8101 aaaagcgccc ggtacggtga tcaacaaatt cattgttctg tacgatattt aactcgtgct
     8161 tgtagcaaat attggtaata cgtgcgatca gacctttttg gtccggacaa atagtacgca
     8221 gaactttacg ttggagtgaa tgcattgctg gaaaaacctt gttgagagtg tttgctaaac
     8281 cgtaccgtca accattattg gccgcagcac tttttaaatt ttttacctga accacaaggg
     8341 cagggatcgt tgcggccaaa ctgcggacgt gtaccgtcaa tatagtacca ttgaccgttt
     8401 tctttcaaaa atcgcgaacg ttcaattatt gcacctgttt tgctaccttc ggtaaatcgc
     8461 gcaacaaaac tgacaaatcc gatattgtcg acgtcctgcc aacaatgttc aaatacggtc
     8521 aatcccaacc attcagtatg cgcaaatccg gcaatcaatt cggcacgtaa cgtcgccgct
     8581 ccacaagatg gatgccaggt ctttattaaa taatctgcgt ctttcatcac aaaagcgcag
     8641 taacgcgaac gcatgagatg ttctggatca ggtgcaacct tttcaccaga cacataaggg
     8701 tggcaacata ggctatactc gacagcacta ccacagggac aaagctgaga cacaaataat
     8761 ctccctggaa acaataacgg cgtattaacc gcctgagtag cactatgtta accgagcagt
     8821 agcgatgttg ctacgattgc attccagggg aatcttgcgg gaataatgag aaagataaaa
     8881 atagggctgg cgctgggatc tggcgcggcg agaggttggt cgcatattgg cgttattaat
     8941 gcgctaaaaa aagtgggtat tgaaattgat atcgttgcag gatgttcaat tggttcgctg
     9001 gtgggcgctg cctatgcatg cgatcgacta tctgcgctgg aagattgggt gacctctttc
     9061 agttattggg atgttttacg cctgatggat ctctcctggc agcgcggtgg gttactgcgc
     9121 ggcgagcgtg tcttcaatca atatcgcgaa ataatgccgg aaacagagat cgaaaattgt
     9181 tcccgtcgct tcgcggctgt tgccaccaat ttaagtactg ggcgcgaatt atggtttact
     9241 gaaggcgatc tccatcttgc tattcgtgca tcatgcagta ttccaggact aatggcaccc
     9301 gttgcgcata acggctactg gctggttgat ggcgctgtag ttaacccaat tcctatttcc
     9361 ctcacgcgtg cattgggggc tgatattgtg atagcggtcg acctgcagca cgatgctcat
     9421 ttgatgcaac aagatttgct ctcctttaat gtcagtgaag aaaatagcga gaatggtgat
     9481 tctctgccgt ggcatgcgcg tctgaaagaa aggttaggca gcataacgac acgtcgggcg
     9541 gtgacagcgc caacggcaac agagattatg accacttcta tccaggtgct ggagaaccgc
     9601 cttaaaagga accgcatggc aggtgatccg cccgatattc tgattcaacc tgtttgcccg
     9661 caaatatcta cgcttgattt ccatcgcgcg cacgctgcca ttgcggccgg gcagctggca
     9721 gtggaaaaga aaatggacga acttttgccg ttggtacgca ccaacatttg accagaattt
     9781 ttatctacac ttaagttaat tctgacaggc gcaggtggca atagcatgcc actattgagt
     9841 aaagccagtc aggggagaga acatgacgca gccattggtc ggaaaacaga ttctcattgt
     9901 tgaagatgag caggtatttc gctcgcttct ggattcgtgg ttttcctcat tgggagcgac
     9961 aacggtactg gcggctgatg gggtggatgc ccttgagttg ctgggaggtt tcactccaga
    10021 cctgatgata tgtgatatcg cgatgccacg aatgaacggg cttaaactgc tggagcatat
    10081 acgtaacaga ggcgaccaga ccccagttct ggtgatatct gccactgaaa atatggcaga
    10141 tattgccaaa gcgttacgtc tgggcgttga agatgttttg ctgaaaccag ttaaagatct
    10201 gaatcgcttg cgcgagatgg tttttgcctg tctctatccc agcatgttta attcccgcgt
    10261 tgaggaagag gaaaggcttt ttcgcgactg ggatgcaatg gttgataacc ctgccgcagc
    10321 ggcgaaatta ttacaggaat tgcaaccgcc ggttcagcag gtgatttccc attgccgggt
    10381 taattatcgt caattggttg ccgcggacaa acccggcctg gtgcttgata ttgccgcact
    10441 ttcggaaaac gatctggcat tttactgcct tgatgtcacc cgagctggac ataatggcgt
    10501 acttgctgcc ttgttattac gcgcattgtt taacggatta ttacaggaac agcttgcaca
    10561 ccaaaatcaa cgtttgccag agttgggcgc gttattgaag caggtaaacc atttactccg
    10621 tcaggccaat ctgccggggc agtttccgct attagttggc tattatcatc gcgaactgaa
    10681 aaatctcatt ctggtttctg cgggtctgaa tgcgacgtta aataccggcg aacaccaggt
    10741 gcaaatcagt aatggtgttc cgttaggcac tttaggtaac gcttatttga atcaattgag
    10801 ccagcgatgc gatgcctggc aatgccaaat atggggaacc ggtggtcggc ttcgcttgat
    10861 gttgtctgca gaatgagcaa acgataacgc gggctaaatt tgcattacct gctaatgtcg
    10921 gctggtggta ctatcgtcgc cattcgtata agtaattgtc ttaattatgc taactcgcct
    10981 ccttttcaga acttagcccc ttcggggtgc tgatatactg ggatgcgata cagaaatatg
    11041 aacacgttca aaacacgaac agtccaggag aatttaaatg gctgccatta atacgaaagt
    11101 caaaaaagcc gttatccccg ttgcgggatt aggaaccagg atgttgccgg cgacgaaagc
    11161 catcccgaaa gagatgctgc cacttgtcga taagccatta attcaatacg tcgtgaatga
    11221 atgtattgcg gctggcatta ctgaaattgt gctggttact cactcatcta aaaactctat
    11281 tgaaaaccac tttgatacca gttttgaact ggaagcaatg ctggaaaaac gtgtaaaacg
    11341 tcaactgctt gatgaagtgc agtctatttg tccgccgcac gtgactatta tgcaagttcg
    11401 tcaggggctg gcgaaaggcc tgggacatgc ggtattgtgt gctcacccgg tagtgggtga
    11461 tgaacctgtg gctgttattt tgcctgacgt tattctggat gaatatgaat ccgatttgtc
    11521 acaggataac ctggcagaga tgatccgccg ctttgatgaa acgggtcata gccagatcat
    11581 ggttgaaccg gttgctgatg ttaccgcata tggcgttgtg gattgcaaag gcgttgaatt
    11641 agcgccgggt gaaagcgtac cgatggttgg tgtggttgaa aaaccaaaag cggatgttgc
    11701 gccgtctaat ctcgctattg tgggtcgtta cgtacttagc gcggatattt ggccgttgct
    11761 ggcaaaaacc cctccgggag ctggtgatga aattcagctc accgacgcaa ttgatatgct
    11821 gatcgaaaaa gaaacggtgg aagcctatca tatgaaaggg aagagccatg actgtggtaa
    11881 taaattaggt tacatgcagg ccttcgttga atacggtatt cgtcataaca cccttggtac
    11941 ggaatttaaa gcctggcttg aagaagagat gggcattaag aagtaacatc cgtatcggtg
    12001 ttatccacga aacggcgttg agcaatcgac gccgtttttt tatagcttat tcttattaaa
    12061 ttgtcttaaa ccggacaata aaaaatcccg ccgatggcgg gatttttaag caagtgcaat
    12121 ctacaaaaga ttattgcttg atcaggaaat cgtcgaggga tttaccttgc tcatccattg
    12181 cttttttgat tacagctgga gtacggccct ggccagtcca ggttttagtt tcgccgtttt
    12241 cgtcaacgta gctatatttt gccggacgct gagcacgttt agctttggtg ccagatttaa
    12301 cggcagcaag gctattcagc agttcgttcg ggtcaatacc gtcagcgatc agcatttcgc
    12361 gatattgctg cagtttacga gtgcgctctt caacttcagc agcagccgcg ctttcttctt
    12421 cgcgacgttc gttaacaaca acttctaatt tttccagcat ttcttccagc gtttcaagtg
    12481 tacattctct tgcctgcgca cgaagagtac ggatgttgtt cagaatttta agtgcttcgc
    12541 tcattgtagt aatctcaaac ttatattggg gtggtttgtt gaggtaataa tagagcctta
    12601 aattcagttg tgcaatagcc aggaatgtaa ggaattcaaa attgttcttt attttgtgcc
    12661 gccaataaat atcttttcat aaaattagcc agaaaagacg cggcatatag ccctatttgc
    12721 gccgatgatt tcgcagcacg tgaggttaaa acttcctgat tcatgtcaca ttttatgggg
    12781 agattatcgt aggctgacga cctttcagtc ttctgtatta gtcgtgttta caagaattcc
    12841 ctattaagcg aatgatgaaa agtagaacag tcgcaataag agcagggact tagtattgca
    12901 ctatctcctg gaggtcaaca gagggctatt acttgcgcaa caggttaaag attgtgaata
    12961 gttaccagca gtcatttacc cgcttataac aagcgaggca gttgtaatga tagctcagaa
    13021 ggattatgca aggcttcgta agggagaacg catataccac ttctgtgcat actgttgagc
    13081 tgaaaaactg acgaattatg ataaactcca gccaacttta attcatatca ttgagggcct
    13141 gtggctgatg gcacagctat atttctacta ttccgcaatg aatgcgggta agtctacagc
    13201 attgttgcaa tcttcataca attaccagga acgcggcatg cgcactgtcg tatatacggc
    13261 agaaattgat gatcgctttg gtgccgggaa agtcagctcg cgtataggtt tgtcatcgcc
    13321 tgcaaaatta tttaaccaaa attcatcatt atttgatgag attcgtgcgg aacatgaaca
    13381 gcaggcaatt cattgcgtac tggttgatga atgtcagttt ttaaccagac aacaagtata
    13441 tgaattatcg gaggttgtcg atcaactcga tatacccgta ctttgttatg gtttacgtac
    13501 cgattttcga ggtgaattat ttattggcag ccaatactta ctggcatggt ccgacaaact
    13561 ggttgaatta aaaaccatct gtttttgtgg ccgcaaagca agcatggtgc tgcgtcttga
    13621 tcaagcaggc agaccttata acgaaggtga gcaggtggtt attggtggta atgaacgcta
    13681 cgtttctgta tgccgtaaac actataaaga agcgttagaa gtcggctcat taacggctat
    13741 tcaggaaagg catcgccacg attaataaga atttctttac tgacagggtg agcagggcgc
    13801 ttttatcctg tcagttcgtt ttactcactt tatccggtgt atataccctt ctcgacagtt
    13861 ctttaacgcc gctatacccc tcacagagcg cttttacagt gacgttgact tgtgacctgt
    13921 aaagtacaat atcgctgtgt gtaggcgtta tacatcgccg taaatatgat gcaggcaaat
    13981 gctgtcggtt tatgggaaag ttgctttggg taaaaaaaac ggccccagaa gggataatgc
    14041 cagtcagtta agcaactgac tggctctttt tcggggctgt ggggtatttc cagggcctct
    14101 cctttaccac tctcgggaag gc
//
LOCUS       DAZFLD010000129        13867 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.130, whole genome shotgun sequence.
ACCESSION   DAZFLD010000129 DAZFLD010000000
VERSION     DAZFLD010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13867
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.130"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..389)
                     /locus_tag="ACHKBL_003236"
     CDS             complement(<1..389)
                     /locus_tag="ACHKBL_003236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5820068.1"
                     /translation="MSQNDIIIRTHYKSPHRMHIDSDIPTPSSEPINQFACQLITLLD
                     TSDLSSMLSYCVTQEFTANCRKISQNCYSTALFTINFATSPIHAENILITLHYKKEII
                     SLLLETTPIKANHLRSILDYIEQEQLTT"
     gene            912..2840
                     /gene="thrS"
                     /locus_tag="ACHKBL_003237"
     CDS             912..2840
                     /gene="thrS"
                     /locus_tag="ACHKBL_003237"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006894721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HFO5820069.1"
                     /translation="MPVITLPDGSQRHYDHAVSPMDVALDIGPGLAKACIAGRVNGEL
                     VDACDLIENDAQLSIITAKDEEGLEIIRHSCAHLLGHAIKQLWPHTKMAIGPVIDNGF
                     YYDVDLDRTLTQEDVEALEKRMHELAEKNYDVIKKKVSWHEARETFANRGESYKVSIL
                     DENIAHDDKPGLYFHEEYVDMCRGPHVPNMRFCHHFKLMKTAGAYWRGDSNNKMLQRI
                     YGTAWADKKALNAYLQRLEEAAKRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWTIFRE
                     LEVFVRSKLKEYQYQEVKGPFMMDRVLWEKTGHWDNYKDAMFTTSSENREYCIKPMNC
                     PGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IRDEVNGCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAEADLAVALEENNI
                     PFEYQLGEGAFYGPKIEFTLYDCLDRAWQCGTVQLDFSLPSRLSASYVGEDNERKVPV
                     MIHRAILGSMERFIGILTEEFAGFFPTWLAPVQVVIMNITDSQSDYVNELTQKLSNAG
                     IRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKVAVRTRRGKDLGSMDVNEV
                     IEKLQQEIRSRSLKQLEE"
     gene            2844..3386
                     /gene="infC"
                     /locus_tag="ACHKBL_003238"
     CDS             2844..3386
                     /gene="infC"
                     /locus_tag="ACHKBL_003238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HFO5820070.1"
                     /translation="MKGGKRVQTARPNRINGEIRAQEVRLTGLEGEQLGIVSLREALE
                     KAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQKVIQVKEIKFRPGT
                     DEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVES
                     FPTKIEGRQMIMVLAPKKKQ"
     gene            3483..3680
                     /gene="rpmI"
                     /locus_tag="ACHKBL_003239"
     CDS             3483..3680
                     /gene="rpmI"
                     /locus_tag="ACHKBL_003239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HFO5820071.1"
                     /translation="MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHL
                     RPKAMVSKGDLGLVIACLPYA"
     gene            3734..4090
                     /gene="rplT"
                     /locus_tag="ACHKBL_003240"
     CDS             3734..4090
                     /gene="rplT"
                     /locus_tag="ACHKBL_003240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HFO5820072.1"
                     /translation="MARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFTALVEKAKAALA"
     misc_feature    4207..4331
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            4213..4257
                     /gene="pheM"
                     /locus_tag="ACHKBL_003241"
     CDS             4213..4257
                     /gene="pheM"
                     /locus_tag="ACHKBL_003241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pheST operon leader peptide PheM"
                     /protein_id="HFO5820073.1"
                     /translation="MNAAIFRFFFYFST"
     gene            4541..5524
                     /gene="pheS"
                     /locus_tag="ACHKBL_003242"
     CDS             4541..5524
                     /gene="pheS"
                     /locus_tag="ACHKBL_003242"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HFO5820074.1"
                     /translation="MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTT
                     LRELPPEERPAAGAVINEAKEQVQQALNARKAELESAALNARLAAETIDVSLPGRRIE
                     NGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWF
                     DATRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNI
                     SFTNLKGTLHDFLRNFFEEDLQIRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVH
                     PNVLRNVGIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDLRFLKQFK"
     gene            5539..7926
                     /gene="pheT"
                     /locus_tag="ACHKBL_003243"
     CDS             5539..7926
                     /gene="pheT"
                     /locus_tag="ACHKBL_003243"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HFO5820075.1"
                     /translation="MKFSELWLREWVNPAIDSDALANQITMAGLEVDGVEPVAGSFHG
                     VVVGEVIECAQHPNADKLRVTKVNVGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGD
                     FKIKAAKLRGEPSEGMLCSFSELGISDDHSGIIELPADAPIGTDIREYLKLDDNTIEI
                     SVTPNRADCLGIIGVARDVAVLNQLPLVEPEIVPVGAAIDDTLPITVEAPEACPRYLG
                     RVVKGINVKAPTPLWMKEKLRRCGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGI
                     VVRMAKEGETLVLLDGTEAKLNADTLVIADHNKALAMGGIFGGEHSGVNDETQNVLLE
                     CAFFSPLSITGRARRHGLHTDASHRYERGVDPALQHKAMERATRLLIDICGGEAGPVI
                     DITNEATLPKRATITLRRSKLDRLIGHHIADEQVTDILRRLGCEVTEGKDEWQAVAPS
                     WRFDMEIEEDLVEEVARVYGYNNIPDEPVQASLIMGTHREADLSLKRVKTLLNDKGYQ
                     EVITYSFVDPKVQQMIHPGVEALLLPSPISVEMSAMRLSLWTGLLATVVYNQNRQQNR
                     VRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESV
                     LDLTGKLNEVEFRAEANPALHPGQSAAIYLKGERIGFVGVVHPELERKLDLNGRTLVF
                     ELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPAADILSECKKVGVNQVVGVNL
                     FDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD"
     gene            7931..8230
                     /gene="ihfA"
                     /locus_tag="ACHKBL_003244"
     CDS             7931..8230
                     /gene="ihfA"
                     /locus_tag="ACHKBL_003244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HFO5820076.1"
                     /translation="MALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEDIPITARRVVTFRPGQKLKSRVENASPKDE"
     gene            8331..9311
                     /gene="btuC"
                     /locus_tag="ACHKBL_003245"
     CDS             8331..9311
                     /gene="btuC"
                     /locus_tag="ACHKBL_003245"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter permease BtuC"
                     /protein_id="HFO5820077.1"
                     /translation="MLTLARQQQRQNIRWLLCLSVLMLLALLLSLCAGELWISPGDWF
                     SPRGELFVWQIRLPRTLAVLLVGAALAISGAVMQALFENPLAEPGLLGVSNGAGVGLI
                     AAVLLGQGQLPNWALGLCAIAGALIITLILLRFARRHLSTSRLLLAGVALGIICSALM
                     TWAIYFSTSVDLRQLMYWMMGGFGGVDWRQSWLMLALIPVLLWICCQSRPMNMLALGE
                     ISARQLGLPLWFWRNVLVAATGWMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRVLL
                     PGCALAGASALLLADIVARLALAAAELPIGVVTATLGAPVFIWLLLKAGR"
     gene            9374..9925
                     /gene="btuE"
                     /locus_tag="ACHKBL_003246"
     CDS             9374..9925
                     /gene="btuE"
                     /locus_tag="ACHKBL_003246"
                     /EC_number="1.11.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional thioredoxin/glutathione peroxidase"
                     /protein_id="HFO5820078.1"
                     /translation="MQDSILTTVVKDIDGEVTTLEKYAGNVLLIVNVASKCGLTPQYE
                     QLENIQKAWADRGFVVLGFPCNQFLEQEPGSDEEIKTYCTTTWGVTFPMFSKIEVNGE
                     GRHPLYQKLIAAAPTAVAPEESGFYARMVSKGRAPLYPDDILWNFEKFLVGRDGLVIQ
                     RFSPDMTPEDPIVMESIKLALAK"
     gene            9925..10674
                     /gene="btuD"
                     /locus_tag="ACHKBL_003247"
     CDS             9925..10674
                     /gene="btuD"
                     /locus_tag="ACHKBL_003247"
                     /EC_number="7.6.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015889 - cobalamin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter ATP-binding protein
                     BtuD"
                     /protein_id="HFO5820079.1"
                     /translation="MSIVMQLQDVAESTRLGPLSGEVRAGEILHLVGPNGAGKSTLLA
                     RMAGMTSGKGSIQFAGQPLEAWSATKLALHRAYLSQHQTPPFAMPVWHYLTLHQHDKT
                     RTELLNDVAGALALDDKLGRSTNQLSGGEWQRVRLAAVVLQITPQANPAGQLLFLDEP
                     MNSLDVAQQSALDKILSALCQQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRRE
                     EVLTPPNLAQAYGMNFRRLDIEGHRMLISTI"
     gene            10753..11217
                     /gene="nlpC"
                     /locus_tag="ACHKBL_003248"
     CDS             10753..11217
                     /gene="nlpC"
                     /locus_tag="ACHKBL_003248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFO5820080.1"
                     /translation="MRFCLILITALFLAGCSHHKAPPPNARLSDSITVIAGLNDQLQS
                     WHGTPYRYGGMTRRGVDCSGFVVVTMRDRFDLQLPRETKQQASIGTQIDKDELLPGDL
                     VFFKTGSGQNGLHVGIYDTNNQFIHASTSKGVMRSSLDNVYWQKNFWQARRI"
     gene            11464..12177
                     /gene="ydiV"
                     /locus_tag="ACHKBL_003249"
     CDS             11464..12177
                     /gene="ydiV"
                     /locus_tag="ACHKBL_003249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-FlhDC factor"
                     /protein_id="HFO5820081.1"
                     /translation="MKIFLENLYHSDCYFLPIRDNQQDLVGVELITHFSSEDGTVRIP
                     TSRVIAQLTEEQHWQLFSEQLELLKSCQHFFIQHKLFAWLNLTPQVATLLLDRDNYAG
                     ELLKYPFIELLINENYPHLDEGKDNRDLLSLSQMYPLVLGNLGAGNSTMKAVFDGLFT
                     RVMLDKSFIQQQITHRSFEPFIRAIQAQISPCCNCIIAGGIDTPEIMAQITPFDFHAL
                     QGCLWPAVPVNQITTLVQR"
     gene            12240..13676
                     /gene="selO"
                     /locus_tag="ACHKBL_003250"
     CDS             12240..13676
                     /gene="selO"
                     /locus_tag="ACHKBL_003250"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0070733 - AMPylase activity [Evidence
                     IEA];
                     GO_process: GO:0018117 - protein adenylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase SelO"
                     /protein_id="HFO5820082.1"
                     /translation="MTLSFVTRWRDELPETYTALSPTPLNNARLIWHNTELANTLSIP
                     SSLFKNGAGVWGGETLLPGMSPLAQVYSGHQFGVWAGQLGDGRGILLGEQRLADGTTM
                     DWHLKGAGLTPYSRMGDGRAVLRSTIRESLASEAMHYLGIPTTRALSIVTSDSPVYRE
                     TVEPGAMLMRVALSHLRFGHFEHFYYRREPEKVRQLADFAIRHYWSHLEDDEDKYRLW
                     FSDVVARTASLIAQWQTVGFAHGVMNTDNMSLLGLTLDYGPFGFLDDYEPGFICNHSD
                     HQGRYSFDNQPAVALWNLQRLAQTLSPFVAVDALNEALDSYQQVLLTHYGQRMRQKLG
                     FMTEQKEDNALLNELFSLMARERSDYTRTFRMLSLTEQHSAASPLRDEFIDRAAFDDW
                     FARYRRRLQQDEVSDIERQQLMQSVNPALVLRNWLAQRAIEAAEKGDMTELHRLHEAL
                     RNPFSDRDDDYASRPPDWGKRLEVSCSS"
     gene            complement(13680..>13867)
                     /gene="hemP"
                     /locus_tag="ACHKBL_003251"
     CDS             complement(13680..>13867)
                     /gene="hemP"
                     /locus_tag="ACHKBL_003251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HFO5820083.1"
                     /translation="YTDSRKLTPETDANHKTASPQPIRRITSQTLLGPDGKLIIDHDG
                     QEYLLRKTQAGKLLLTK"
BASE COUNT         3457 a         3222 c         3710 g         3478 t
ORIGIN      
        1 gtagttaact gttcctgttc aatataatcc agtatgcttc gcaaatggtt agctttaata
       61 ggcgtggttt ccagtaataa ggaaatgatt tcttttttat agtgtaatgt aatgagtata
      121 ttttctgcat ggatgggtga agtggcaaag ttaatggtaa aaagggcagt ggaataacaa
      181 ttttgtgata tttttcgaca gtttgcggta aattcctgag taacacagta tgacagcatt
      241 gaacttaagt cagaggtatc aagtagggtg atgagttggc acgcaaattg attaataggc
      301 tctgatgaag gcgttggtat gtcgctatcg atgtgcattc tatgaggaga cttataatga
      361 gttctgataa taatgtcgtt ttgactcata atctattcgc cttatatatg tctggaggtt
      421 ttgaggatga tgattaatta ttatcgccag aacgaaatgt gcacgaaaaa ttgctataag
      481 aattgtgtca cttttatcat tgtgatatga ccacttcgtc attaatctta tgatcgttta
      541 aatagtaccg ccaaattacg caacgaactt tgctggattc agtttatgct gctgcaaatc
      601 cgctcgcagt aaacctttca gacgctccgt gatgttatca gtggttcttt aagcgttttg
      661 ctggtgtact cactacaaac gaattgcgaa tcgaatcaat gtgaaacgga aaggtacaat
      721 ctcccttttt ttagtgttaa catcgttcaa ccgggtggaa ggtgagaggt taatgtcttt
      781 catttgtgta cctgattgcg aaccaattta gcatttgttg gctagatggt ttcgcaatga
      841 actgttaata aacaaatttt tctttgtatg tgatctttcg tgtgggtcac cactgcaaat
      901 aaggatataa aatgcctgtt ataactcttc ctgatggcag ccaacgccat tacgaccacg
      961 ctgtaagccc catggatgtt gcgctggaca ttggtccagg tctggcgaaa gcctgtatcg
     1021 cagggcgcgt taatggcgaa ctggttgatg cttgcgatct gattgaaaac gacgcacaac
     1081 tgtcgatcat taccgccaaa gacgaagaag gtctggagat cattcgtcac tcctgtgcgc
     1141 acctgttagg gcacgcgatt aaacaacttt ggccgcatac caaaatggca atcggcccgg
     1201 ttattgacaa cggtttttat tacgacgttg atcttgaccg cacgttaacc caggaagatg
     1261 tcgaagcact cgagaagcgg atgcatgagc ttgctgagaa aaactacgac gtcattaaga
     1321 agaaagtcag ctggcacgaa gcgcgtgaaa ctttcgccaa ccgtggtgag agctacaaag
     1381 tctccattct tgacgaaaac atcgcccatg atgacaagcc aggtctgtac ttccatgaag
     1441 aatatgtcga tatgtgccgc ggtccgcacg taccgaacat gcgtttctgc catcatttca
     1501 aactaatgaa aacggcaggg gcttactggc gtggcgacag caacaacaaa atgttgcaac
     1561 gtatttacgg tacggcgtgg gcagacaaaa aagcgcttaa cgcttacctg cagcgcctgg
     1621 aagaagccgc gaaacgcgac caccgtaaaa tcggtaaaca gctcgacctg taccatatgc
     1681 aggaagaagc accgggtatg gtattctggc acaacgatgg ctggaccatc ttccgtgaac
     1741 tggaagtgtt tgttcgttct aaactgaaag agtaccagta tcaggaagtt aaaggtccgt
     1801 tcatgatgga ccgtgtcctg tgggaaaaaa ccggtcactg ggacaactac aaagatgcaa
     1861 tgttcaccac gtcttctgag aaccgtgaat actgcattaa gccgatgaac tgcccgggtc
     1921 acgtacaaat tttcaaccag gggctgaagt cgtatcgcga tttgccgctg cgtatggccg
     1981 agtttggtag ctgccaccgt aacgagccgt caggttcgct gcatggcctg atgcgtgtac
     2041 gtggttttac ccaggatgac gcgcatatct tctgtactga agaacaaatt cgcgatgaag
     2101 ttaacggatg tatccgttta gtctatgata tgtacagcac ttttggcttc gagaagatcg
     2161 tcgtcaaact ctccactcgt cctgaaaaac gtattggcag cgacgaaatg tgggatcgtg
     2221 ctgaggcgga cctggcagtt gcgctggaag aaaacaacat cccgtttgaa tatcaactgg
     2281 gtgaaggcgc tttctacggt ccgaaaattg aatttaccct gtatgactgc ctcgatcgtg
     2341 catggcagtg cggtacagta cagctggact tctccttgcc gtctcgtctg agcgcctcct
     2401 atgtgggcga agacaacgag cgtaaggtac cggtaatgat tcaccgcgca attcttgggt
     2461 cgatggaacg tttcatcggt atcctgaccg aagaattcgc tggtttcttc ccgacctggc
     2521 ttgcgccggt tcaggttgtt atcatgaata ttaccgattc acagtctgat tacgttaacg
     2581 aattgacgca aaaactatca aatgcgggca ttcgtgttaa agcagacttg agaaatgaga
     2641 agattggctt taaaatccgc gagcacactt tgcgtcgcgt cccgtatatg ctggtctgtg
     2701 gtgataaaga ggtggaatca ggcaaagttg ccgttcgcac ccgccgtggt aaagacctgg
     2761 gaagcatgga cgtaaatgaa gtgatcgaga agctgcaaca agagattcgc agccgcagtc
     2821 ttaaacaatt ggaggaataa ggtattaaag gcggaaaacg agttcaaacg gcgcgcccta
     2881 accgtatcaa tggcgaaatt cgcgcccagg aagttcgctt aacaggtctg gaaggcgagc
     2941 agcttggtat tgtgagtctg agagaagctc tggagaaagc agaagaagcc ggagtagact
     3001 tagtcgagat cagccctaac gccgagccgc cggtttgtcg tataatggat tacggcaaat
     3061 tcctctatga aaagagcaag tcttctaagg aacagaagaa aaagcaaaaa gttatccagg
     3121 ttaaggaaat taaattccgt cctggtacag atgaaggcga ctatcaggta aaactccgca
     3181 gcctgattcg ctttctcgaa gagggtgata aagccaaaat cacgctgcgt ttccgcggtc
     3241 gtgagatggc gcaccagcaa atcggtatgg aagtgcttaa tcgcgtgaaa gacgatttgc
     3301 aagaactggc agtggtcgaa tccttcccaa cgaagatcga aggccgccag atgatcatgg
     3361 tgctcgctcc taagaagaaa cagtaaggcc ttcaagtagc actgtctgtg gagcctttgg
     3421 gcttcacagg cattgttcgc ctttgtttcg tttattaaca atgcgaagtg gaagttatta
     3481 aaatgccaaa aattaagacc gtacgcggtg ctgctaagcg cttcaaaaaa accggtaaag
     3541 gtggttttaa gcataagcac gctaacctgc gtcacattct gaccaaaaaa gcgaccaaac
     3601 gtaaacgtca cctgcgtccg aaagccatgg tttccaaagg cgatctgggc ctggtaatcg
     3661 cgtgcctgcc gtacgcataa gccgttaacg ttttttaact ttttaattag aatatagata
     3721 caggagagca catatggctc gcgtaaaacg tggtgttatt gcacgcgcac gtcacaagaa
     3781 aattttgaaa caagctaaag gctactacgg tgcgcgttct cgcgtatacc gcgttgcctt
     3841 ccaggctgtt atcaaagctg gtcagtatgc ttaccgtgac cgtcgtcaac gtaagcgtca
     3901 gttccgtcaa ctgtggattg cgcgtatcaa cgcagcagca cgtcagaacg gtatttctta
     3961 cagcaaattc atcaatggcc tgaaaaaagc ctctgttgaa atcgaccgta agatcctggc
     4021 tgatatcgca gtattcgaca aagtagcgtt caccgctctg gttgaaaaag cgaaagcagc
     4081 tctggcataa gccagttgaa agagggagct agtctccctc ttttcgtttc aacgccatca
     4141 aaacattgac ttttatcgcc gtagcctttt caataaaggt cttttgaaga gtaccaaaag
     4201 gtaacgcaag caatgaatgc tgctattttc cgcttctttt tttactttag cacctgactc
     4261 caggaggcta gcgcgtgaga agagaaacgg aaaacagcgc ctgaaagcct cccagtggag
     4321 gctttttttg tatgcgcgtt ttgaaaaata aaaagcctgt ctcatcaggc gtaattgtcg
     4381 cagccagtct ggacacggcc agcgcggaga aaccggagcg tacttaagta cgtgaggatt
     4441 tcgagcactg cccgggacca aaatggcaag taaaatagcc tgatgggatg ggctctaagt
     4501 ccaacgaacc agtgtcacca ctgacacaat gaggaaaaac atgtcacatc tcgcagaact
     4561 ggttgccagt gcgaaggcgg ccattagcca ggcgtcagat gttgccgcgt tagataatgt
     4621 gcgcgtcgaa tatttgggta aaaaagggca cttaaccctt cagatgacga ccctgcgtga
     4681 gctgccgcca gaagagcgtc cggcagctgg tgcggttatc aacgaagcga aagagcaggt
     4741 tcagcaggcg ctgaatgcgc gtaaagcgga actggaaagc gctgcactga atgcgcgtct
     4801 ggcggcggaa acgattgatg tctccctgcc gggtcgtcgc attgaaaacg gcgggctgca
     4861 tccggtgacc cgtaccatcg accgtatcga aagtttcttc ggtgagcttg gctttaccgt
     4921 ggcaaccggg ccggaaatcg aagacgatta tcataacttc gatgctctga acattcctgg
     4981 tcaccacccg gcgcgcgctg accacgacac tttctggttt gacgctaccc gcctgctgcg
     5041 tacccagacc tctggcgtac agatccgcac catgaaagct cagcagccac cgattcgtat
     5101 catcgcgcct ggccgcgttt atcgtaacga ctacgaccag actcacacgc cgatgttcca
     5161 tcagatggaa ggtctgattg ttgataccaa catcagcttt accaacctga aaggcacgct
     5221 gcacgacttc ctgcgtaact tctttgagga agatttgcag attcgcttcc gtccttccta
     5281 cttcccgttt accgaacctt ctgcagaagt ggacgtcatg ggtaaaaacg gtaaatggct
     5341 ggaagtactg ggctgcggga tggtgcatcc gaacgtgctg cgtaacgttg gcatcgaccc
     5401 ggaagtttac tctggtttcg ccttcgggat ggggatggag cgtctgacca tgttgcgtta
     5461 cggcgtcacc gacctgcgtt cattcttcga aaacgatctg cgtttcctca aacagtttaa
     5521 ataaggcagg aatagattat gaaattcagt gaactgtggt tacgcgaatg ggtgaacccg
     5581 gcgattgata gcgatgcgct ggcaaatcaa atcactatgg cgggcctgga agttgacggt
     5641 gtagaaccgg ttgccggtag cttccacggc gtggtcgttg gtgaagtgat tgagtgtgcg
     5701 cagcatccga acgctgacaa actgcgtgtg acaaaagtga atgtcggcgg cgatcgcctg
     5761 ctggacatcg tctgcggtgc gccaaactgc cgtcagggcc tgcgtgtggc ggtagcgacc
     5821 attggtgctg ttctgccggg cgatttcaaa attaaagcgg cgaaactgcg tggcgaaccg
     5881 tctgaaggga tgctgtgctc cttttctgag ctgggaattt ctgacgatca cagcggcatt
     5941 atcgagctgc ctgcagatgc gccgattggc accgacatcc gcgaatacct gaaactcgat
     6001 gacaacacca tcgaaatcag cgtaacgcca aaccgtgccg attgcttagg cattatcggc
     6061 gtggcgcgtg atgttgccgt gctgaaccag ctgccgctgg ttgaaccgga aatcgttccg
     6121 gttggtgcgg ccatcgacga cacgctgccg attacagtcg aagcgccgga agcctgcccg
     6181 cgctatcttg gccgtgtggt aaaaggcatt aacgttaaag cgccaactcc gctgtggatg
     6241 aaagaaaaac tgcgtcgttg cgggatccgt tctatcgatg cagttgttga cgtcaccaac
     6301 tatgtgctgc tcgaactggg ccagccgatg cacgctttcg ataaagatcg cattgaaggc
     6361 ggcattgtgg tgcggatggc gaaagagggc gaaactctgg tgctgcttga tggcactgaa
     6421 gcgaagctga atgctgacac cctggtcatc gccgaccaca acaaggcgct ggcgatgggc
     6481 ggcatctttg gtggcgaaca ctctggcgtg aatgacgaaa cgcaaaacgt cctgctggaa
     6541 tgcgctttct tcagcccgct gtctatcacc ggtcgtgctc gtcgtcatgg cctgcatact
     6601 gatgcgtctc accgttatga gcgtggcgtt gatccggcac tgcagcacaa agcgatggaa
     6661 cgtgcgaccc gtctgctgat cgacatctgc ggtggtgagg ctggtccggt aattgatatc
     6721 accaacgaag caacgctgcc gaagcgtgcc accattactt tacgtcgcag caaactggat
     6781 cgcctgatcg gccatcatat tgctgatgag caggtaactg acattctgcg tcgtctcggc
     6841 tgcgaagtga ccgaaggcaa agacgaatgg caggccgttg cgccgagctg gcgtttcgac
     6901 atggagattg aagaagatct ggtcgaagaa gtcgcgcgtg tttacggcta caacaacatc
     6961 ccggatgagc cggtacaggc aagcctgatt atgggtactc accgcgaagc cgatctgtcg
     7021 ctcaagcgcg tgaaaacgct gctcaacgac aaaggctatc aggaagtgat cacctacagc
     7081 ttcgttgatc cgaaagtgca gcagatgatc catccaggcg ttgaagcctt actgctgcca
     7141 agccctattt ccgttgaaat gtcagcaatg cgtctttccc tgtggaccgg cctgctggca
     7201 accgtggtgt acaaccagaa ccgtcagcag aaccgtgtgc gcattttcga aagtggtctg
     7261 cgtttcgtac cagatactca ggcaccgttg ggcattcgtc aggatctgat gttagccggt
     7321 gtgatttgcg gtaaccgtta cgaagagcac tggaacctgg caaaagagac cgttgatttc
     7381 tatgatttga aaggcgatct tgaatccgtt ctcgacctga ccggtaaact gaatgaggtt
     7441 gagttccgtg cagaagcgaa tccggcactg catccggggc aatccgcagc gatttatctg
     7501 aaaggtgaac gtattggttt tgttggggtt gttcatcctg aactggaacg taaactggat
     7561 cttaacggtc gcactctggt gttcgaactg gagtggaaca agctcgcaga ccgcgtggtg
     7621 cctcaggcgc gcgagatttc tcgcttcccg gcgaaccgtc gtgacatcgc ggtggtggtc
     7681 gcagaaaacg ttcccgcagc ggatatttta tccgaatgta agaaagttgg cgtaaatcag
     7741 gtagttggcg taaacttatt tgacgtgtac cgcggtaagg gtgttgcgga ggggtataag
     7801 agcctcgcca taagcctgat cctgcaagat accagccgta cactcgaaga agaggagatt
     7861 gccgctaccg tcgccaaatg tgtagaggca ttaaaagagc gattccaggc atcattgagg
     7921 gattgaacct atggcgctta caaaagctga aatgtcagaa tatctgtttg ataagcttgg
     7981 gcttagcaag cgggatgcca aagaactggt tgaactgttt ttcgaagaga tccgtcgcgc
     8041 tctggaaaac ggcgaacagg tgaaactctc tggttttggt aacttcgatc tgcgtgataa
     8101 gaatcaacgc ccgggacgta acccgaaaac gggcgaggat attcccatta cagcacggcg
     8161 cgtggtgacc ttcagacccg ggcagaagtt aaaaagccgg gtcgaaaacg cttcgcccaa
     8221 agacgagtaa tctgatctaa ctaaaaaggc cgctctgcgg ccttttttct tttcactgtc
     8281 gaagagtcac cgtaaaatca acgccatgac acttcagcag aacggatacc atgctgacac
     8341 ttgcccgcca acaacagcga caaaatattc gctggttatt atgcctgtca gttttgatgc
     8401 tgctggcgct tctcttaagc ctttgcgccg gtgaactatg gatctcgcca ggtgactggt
     8461 tttctcctcg tggcgaactg ttcgtctggc aaattcgcct gccacgtacg ctggctgtat
     8521 tgctggttgg tgcggcgctg gctatatccg gcgctgtaat gcaggcgttg tttgaaaatc
     8581 ctctggcaga acctggacta cttggcgtct ctaacggcgc aggcgtgggg cttatcgccg
     8641 cggtattgct tgggcaaggg caactcccca actgggcgct agggctgtgt gcgattgctg
     8701 gcgcgcttat catcacttta atactcttac gtttcgctcg tcgtcatctt tcgaccagtc
     8761 ggttattgct ggctggcgtt gcattaggga ttatctgtag cgcactaatg acgtgggcta
     8821 tctacttttc cacctctgtt gatttacgtc agctgatgta ctggatgatg ggcggttttg
     8881 gcggcgtaga ctggcggcaa agctggctga tgctggcatt gatccccgtg ttgttgtgga
     8941 tctgttgtca gtccaggccg atgaatatgt tagcacttgg cgagatctcg gcgcggcaac
     9001 tgggtttacc cctgtggttc tggcgcaatg tgttggtcgc agcgaccggc tggatggttg
     9061 gcgtcagtgt ggcgctggcg ggtgctatcg gctttattgg tctggtgatc ccacatattc
     9121 tccggctgtg tggtttaacc gatcatcgcg tattacttcc cggctgcgcg ctggcagggg
     9181 cgagcgcatt gctgctggcc gatattgtag cgcgcctggc attagctgcc gcagagctgc
     9241 ctattggcgt ggtcaccgca acgttgggtg cgccggtgtt tatctggtta ttgttaaaag
     9301 caggacgtta gccgcaaaaa gacggtctat gattaaaagc taagattttt actgaccaca
     9361 cccaggagaa acgatgcaag attccattct gacgaccgta gtgaaagata tcgacggtga
     9421 agtgaccacg ctggagaagt acgccggtaa tgtgctgttg attgtcaatg tcgcctcaaa
     9481 gtgtggctta acgccgcaat atgagcagtt ggagaatatt cagaaagcct gggccgatcg
     9541 tggttttgtg gtgctgggat tcccgtgcaa ccagtttctg gaacaagaac cgggcagcga
     9601 tgaagagatt aaaacttact gtaccaccac atggggggtg acgttcccga tgttcagtaa
     9661 gattgaagtt aatggcgaag gacgccatcc gctgtatcaa aaattgattg ccgcagcgcc
     9721 gaccgcagtc gcgccggaag agagcggatt ctatgctcgt atggtcagca aaggtcgtgc
     9781 accgctgtac cctgatgata ttttatggaa ttttgagaaa tttttagttg gcagggacgg
     9841 attggtcatc cagcgttttt ccccggatat gacgccggaa gatcccattg tgatggaaag
     9901 cattaaactg gcgttggcaa aataatgtct attgtgatgc agttacagga tgttgcggaa
     9961 tctacccgcc tggggccgct ttctggcgag gttcgggctg gggagatcct gcacctggtg
    10021 gggccgaatg gcgcgggtaa gagtacctta ctggcgcgaa tggccggaat gaccagcggt
    10081 aagggaagca ttcagttcgc ggggcaacca ctggaagcat ggtccgcaac aaaactcgcg
    10141 ctacatcgcg cctatctttc acaacaccag acgccaccgt ttgcaatgcc ggtctggcac
    10201 tacctgacac tgcatcagca cgataaaacg cgtaccgaac tactgaatga tgtcgcaggg
    10261 gcgctggctc ttgatgacaa actcggacgg agcaccaatc aactttccgg cggtgaatgg
    10321 caacgcgtac gtcttgctgc ggtggtgttg caaatcacac cacaagccaa tcccgcaggc
    10381 caattgctgt ttcttgatga gccgatgaac agtcttgatg ttgcgcaaca aagtgcgtta
    10441 gacaaaattc tgagcgcact gtgtcagcaa ggactggcga ttgtgatgag cagtcacgat
    10501 ctcaaccaca ccttgcgtca tgcgcatcgg gcgtggttgc taaaaggtgg aaaaatgctg
    10561 gccagtggac gcagggaaga ggtgctcacg ccgccaaatc tggcgcaggc ctatgggatg
    10621 aattttcgcc gtctggatat cgaaggtcac agaatgctga tttcgaccat ctgatttcat
    10681 taatatcttg aaaatcgggt aattacgacg ctaaattaat gcaagaataa aaaaacagag
    10741 gaaccgtacg gaatgcgttt ctgccttatt ttgatcacag cactgtttct ggccgggtgt
    10801 agccaccata aagcaccgcc gccaaatgcc agactttctg attcgattac cgttattgcc
    10861 ggtttgaacg accagctaca aagctggcat ggtacgccgt atcgttatgg tggcatgacg
    10921 cggcgcggtg tggactgttc ggggtttgtg gttgtgacga tgcgcgatcg tttcgatttg
    10981 cagctgcccc gtgaaaccaa acaacaagcc tctatcggca cgcaaattga taaagacgag
    11041 ttgctgcctg gtgacctggt ctttttcaaa acgggctccg gacaaaatgg tttgcatgta
    11101 ggtatttatg ataccaacaa ccaatttatc cacgcctcta ccagcaaggg agtgatgcgt
    11161 tcctcacttg ataatgtcta ttggcagaaa aatttctggc aggcgagacg aatctagtat
    11221 gtcgaaaaat ggacggcgat gatacatcgc cgtccattta ctcctgaata cagtagtatt
    11281 ttgcagacaa aaattccgta aatggattat tcgtttataa tcaggataat ctaaaaaaag
    11341 gacccctgaa aaagtggtta acatggaaca aatgaaagtt actccttctg taattgttat
    11401 gtcttatggc ctcatccctt ttagccggat actgaaaaac atccttcgag agggacggtt
    11461 accatgaaga tttttttgga gaatctttat cattctgatt gctatttcct gccgataaga
    11521 gataaccagc aagaccttgt cggcgttgag ttaattaccc atttctccag tgaagatggt
    11581 acggttcgta tcccgacgag tcgggttata gcgcaactta ccgaggagca acattggcaa
    11641 ttattttcag agcagttgga attactaaaa tcatgccaac acttttttat tcaacataaa
    11701 ctctttgcct ggcttaattt aacgccacaa gtggctacat tattattaga cagggataat
    11761 tacgcaggcg aactattaaa atatccgttt attgaactgt tgattaatga gaattacccg
    11821 catctcgatg aaggaaaaga taatcgcgat cttttgtcct tatcgcaaat gtatcccctt
    11881 gtgttaggta atttgggcgc aggcaatagc acaatgaaag cggtttttga tggtcttttc
    11941 acccgcgtca tgctggataa aagttttatt cagcaacaaa tcacgcatcg ctccttcgaa
    12001 ccgtttattc gtgcgatcca ggctcaaatt tccccatgtt gcaattgcat catcgcgggg
    12061 ggcattgata cgccagaaat aatggcacag attaccccct ttgatttcca tgctctccag
    12121 ggatgcctgt ggcctgccgt tcctgtaaat cagataacga cgttggttca gcgataaccc
    12181 tcctgtttgc tggtgtttaa gacgagagta accgtctaca ctatcaaata ggaggatcta
    12241 tgaccctgtc ttttgttacc cgctggcgcg atgaattgcc agaaacctat acagcacttt
    12301 cccctacgcc tttaaataat gcccggctga tttggcataa taccgaactc gctaacacgc
    12361 tgagtattcc atcgtcgctg tttaaaaatg gcgcaggtgt ctggggcggc gaaaccctac
    12421 tgcctggtat gtcaccactg gcgcaggttt acagtggtca tcagttcggc gtctgggcgg
    12481 gccaactggg tgatgggcgc ggcattttac tcggcgaaca acggcttgct gatggcacta
    12541 caatggactg gcatctgaaa ggtgctggcc tgacgcctta ttcgcgaatg ggtgatggac
    12601 gggcggtttt acgttcgacg atacgagaaa gtctcgccag tgaggcgatg cattatctgg
    12661 gcattccgac gacccgcgcg ttaagtatcg tcaccagcga ttcgccagtg tatcgggaaa
    12721 cggtggagcc aggcgcgatg ctgatgcgtg tggcactaag tcatctgcgc tttggtcatt
    12781 tcgaacattt ttactatcgc cgcgagccgg aaaaggttcg tcagttggct gactttgcca
    12841 ttcgtcatta ctggtctcat cttgaagatg atgaggacaa ataccgtctc tggtttagcg
    12901 atgttgtcgc acgtaccgca tcgttaattg cccaatggca gacggtcggc tttgctcatg
    12961 gggtgatgaa taccgacaac atgtcgctgc tggggctgac gcttgattac gggccgtttg
    13021 gttttcttga tgattacgaa cccggtttta tttgtaatca ctcggatcac caagggcgtt
    13081 acagctttga taatcaacct gccgtcgcgt tgtggaattt acagcgtctg gcgcagacat
    13141 tgtcgccatt tgtcgccgtt gatgccctga atgaggccct ggacagctat cagcaggttt
    13201 tgttgacgca ttatggacaa cggatgcggc agaaactggg cttcatgacg gagcaaaaag
    13261 aggataacgc gctactaaat gaattattca gtctgatggc gcgagagcgc agcgattata
    13321 cccgcacatt tcgcatgctg agtctgaccg agcagcacag cgcggcgtca ccgctacgtg
    13381 atgagtttat tgatcgtgcg gcatttgatg actggtttgc ccgttatcgg aggcgtttgc
    13441 aacaagacga ggttagcgat attgagcgtc agcaactgat gcaaagcgtt aacccagctc
    13501 tggtgttgcg caactggttg gcgcaacggg cgattgaagc ggcagaaaag ggtgatatga
    13561 cggaattgca ccgcctgcat gaggcgttgc gaaatccttt cagcgacaga gatgatgact
    13621 acgccagtcg tccacctgac tggggtaaac ggctggaagt cagctgctcg agttaaaggt
    13681 tacttggtca acagcagctt gccagcctga gttttacgga gcagatattc ttgcccgtca
    13741 tgatcgataa tcagtttgcc atccggacct aacagtgtct ggctggtaat tcgccggatg
    13801 ggctgcgggg aagcggtctt gtgattggca tccgtttcag gcgtgagttt tctgctatcc
    13861 gtataac
//
LOCUS       DAZFLD010000130        13843 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.131, whole genome shotgun sequence.
ACCESSION   DAZFLD010000130 DAZFLD010000000
VERSION     DAZFLD010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13843
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.131"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            82..1215
                     /gene="tssD"
                     /locus_tag="ACHKBL_003252"
     CDS             82..1215
                     /gene="tssD"
                     /locus_tag="ACHKBL_003252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tube protein TssD"
                     /protein_id="HFO5820084.1"
                     /translation="MSDIIYLTITGEQQGCISSRCGTSASIGNRWQIGHEDEIFAFSL
                     SNSITNTGKGSQLHGLSFCKLIDKSSPLLINAINNNEQLFMEFDFYRINRFGRWEKYY
                     NIQLRGALLSAINHLFTENNLDTETITVSYEYILSRHLIANTEFSHLAFPDNYNRLFI
                     PRQKTKDDNGLKTLNSKAVGRLLAAGGIYNGNIEGFRETANKLGGDAPAGYDQVMDNK
                     GLLIAGASIVAGVVLGRMRFPDLESFEHFGARGAVSGRPFKPDLAGGPIENLTIDGVT
                     INREGIAIVEKHISRFDHDPANDVMISRLKKIANKELLPEKYDLNYYTHECREYQRYC
                     NLGWETGEPNGLDGYELWNNAHTATLEDFKIKDTDLFHPDAKK"
     gene            1212..1469
                     /locus_tag="ACHKBL_003253"
     CDS             1212..1469
                     /locus_tag="ACHKBL_003253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFO5820085.1"
                     /translation="MTITIVEFNVLKVMAEKDIDWSWMVLDRTLAIRNIPGFGNVANI
                     VTKLVNHGLVDIVNGEGNSKPRYRVSQYGLNLIKEQQDNLF"
     gene            1544..1789
                     /locus_tag="ACHKBL_003254"
     CDS             1544..1789
                     /locus_tag="ACHKBL_003254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFO5820086.1"
                     /translation="MITKNELNVLNVMTEKDINWNWMNLDRTLSIRQIPGFGNVVNIV
                     NKLANEGLVIIEDSGHKSMPHYHVSEKGYNLVQRENK"
     gene            complement(1958..4219)
                     /gene="metE"
                     /locus_tag="ACHKBL_003255"
     CDS             complement(1958..4219)
                     /gene="metE"
                     /locus_tag="ACHKBL_003255"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HFO5820087.1"
                     /translation="MTILNHTLGFPRVGLRRELKKAQESYWAGNSTREELLAVGRELR
                     ARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVPPRHQNKDGSVDIDTLFRIGRG
                     RAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLEEVDEALALGHKVKPV
                     LLGPVTYLWLGKVKGEQFDRLSLLNDILPVYQQVLAELAKRGIEWVQIDEPALVLELP
                     QAWLDAYKPAYDALQGQVKLLLTTYFEGVTPNLDTITALPVQGLHVDLVHGKDDVAEL
                     HKRLPSDWLLSAGLINGRNVWRADLTEKYAQIKDIVGKRDLWVASSCSLLHSPIDLSV
                     ETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAV
                     EKRLAAITAQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKG
                     NLDANNYRTGIAEHIKQAIVEQERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGW
                     VQSYGSRCVKPPIVIGDVSRPAPITVEWAKYAQSLTDKPVKGMLTGPVTILCWSFPRE
                     DVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEA
                     FRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDY
                     PNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAAL
                     ANMVQAAQNLRRG"
     gene            4337..5290
                     /gene="metR"
                     /locus_tag="ACHKBL_003256"
     CDS             4337..5290
                     /gene="metR"
                     /locus_tag="ACHKBL_003256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MetR"
                     /protein_id="HFO5820088.1"
                     /translation="MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRL
                     GFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQ
                     WLTPALENFHKNWPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
                     YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSV
                     DNTLLLIQMVAARMGIAALPHWVVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQP
                     ITEAFIRSARNHACDHLPFVKSAERPTYDAPTVRPGSPARL"
     gene            complement(5178..6077)
                     /gene="bioP"
                     /locus_tag="ACHKBL_003257"
     CDS             complement(5178..6077)
                     /gene="bioP"
                     /locus_tag="ACHKBL_003257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter"
                     /protein_id="HFO5820089.1"
                     /translation="MALLIITTILWAFSFSFYGEYLAGHVDSYFAVLVRVGLAALVFL
                     PFLRTRGNSLKTVGLYMLVGAMQLGVMYMLSFRAYLYLTVSELLLFTVLTPLYITLIY
                     DIMSKRRLRWGYAFSALLAVIGAGIIRYDQVTDHFWTGLLLVQLSNITFAIGMVGYKR
                     LMETRPMPQHNAFAWFYLGAFLVAVIAWFLLGNAQKMPQTTLQWGILVFLGVVASGIG
                     YFMWNYGATQVDAGTLGIMNNMHVPAGLLVNLAIWHQQPHWPTFITGALVILASLWVH
                     RKWVAPRSSQTADDRRRDCALNE"
     gene            complement(6153..6953)
                     /gene="yigL"
                     /locus_tag="ACHKBL_003258"
     CDS             complement(6153..6953)
                     /gene="yigL"
                     /locus_tag="ACHKBL_003258"
                     /EC_number="3.1.3.23"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar/pyridoxal phosphate phosphatase YigL"
                     /protein_id="HFO5820090.1"
                     /translation="MYQVVASDLDGTLLSPDHTLSPYAKETLKLLTARGINFVFATGR
                     HHVDVGQIRDNLEIKSYMITSNGARVHDLDGNLIFAHNLDRDIASDLFGVVNDNPDII
                     TNVYRDDEWFMNRHRPEEMRFFKEAVFKYALYEPGLLEPEGVSKVFFTCDSHEQLLPL
                     EQAINARWGDRVNVSFSTLTCLEVMAGGVSKGHALEAVAKKLGYSLKDCIAFGDGMND
                     AEMLSMAGKGCIMGSAHQRLKDLHPELEVIGTNAEDAVPHYLRKLYLS"
     gene            complement(6961..7983)
                     /gene="pldB"
                     /locus_tag="ACHKBL_003259"
     CDS             complement(6961..7983)
                     /gene="pldB"
                     /locus_tag="ACHKBL_003259"
                     /EC_number="3.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase L2"
                     /protein_id="HFO5820091.1"
                     /translation="MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVR
                     FVRFRAQHHDRVIVICPGRIESYVKYAELAYDLFHLGFDVLIIDHRGQGRSGRLLADP
                     HLGHVNRFNDYVDDLAAFWQQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
                     LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRY
                     RRNLRFYADDPTIRVGGPTYHWVRESILAGEQVLAGAGDDATPTLLLQAEEERVVDNR
                     MHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALHAIVDFFNRHNSPSG
                     NRSTEV"
     gene            8094..8714
                     /gene="rhtB"
                     /locus_tag="ACHKBL_003260"
     CDS             8094..8714
                     /gene="rhtB"
                     /locus_tag="ACHKBL_003260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
                     /protein_id="HFO5820092.1"
                     /translation="MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIA
                     GLQTGLAIHIVLVGVGLGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRAAGAIDLKPL
                     ASTQSRRHLFQRAVFVNLTNPKSIVFLAALFPQFIMPQQPQLMQYIVLGVTTIVVDII
                     VMIGYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARHA"
     gene            complement(8776..9396)
                     /gene="rhtC"
                     /locus_tag="ACHKBL_003261"
     CDS             complement(8776..9396)
                     /gene="rhtC"
                     /locus_tag="ACHKBL_003261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709629.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine export protein RhtC"
                     /protein_id="HFO5820093.1"
                     /translation="MLMLFLTVAMVHIVALMSPGPDFFFVSQTAVSRSRKEAMMGVLG
                     ITCGVMVWAGIALLGLHLIIEKMAWLHTLIMVGGGLYLCWMGYQMLRSALKKEVVSAP
                     APQVELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTERWGIFALIIIET
                     LAWFTVVASLFALPQMRRGYQRLAKWIDGFAGALFAGFGIHLIISR"
     gene            complement(9460..11289)
                     /gene="recQ"
                     /locus_tag="ACHKBL_003262"
     CDS             complement(9460..11289)
                     /gene="recQ"
                     /locus_tag="ACHKBL_003262"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026263.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HFO5820094.1"
                     /translation="MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCL
                     VVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQANGVAAACLNSTQTRE
                     QQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
                     EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLM
                     EKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAARLQSKGISAAAYHAGLENDVRA
                     DVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPA
                     EAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEG
                     RQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDY
                     GHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSL
                     QLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEM
                     AEQMPISASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE"
     gene            complement(11421..12290)
                     /gene="pldA"
                     /locus_tag="ACHKBL_003263"
     CDS             complement(11421..12290)
                     /gene="pldA"
                     /locus_tag="ACHKBL_003263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004620 - phospholipase activity [Evidence
                     IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HFO5820095.1"
                     /translation="MRTLQGWLLPVFMLPMAVYAQEATVKEVHDAPAVRGSIIANMLQ
                     EHDNPFTLYPYDTNYLIYTQTSDLNKEAIASYDWAENARKDEVKFQLSLAFPLWRGIL
                     GPNSVLGASYTQKSWWQLSNSEESSPFRETNYEPQLFLGFATDYRFAGWTLRDVEMGY
                     NHDSNGRSDPTSRSWNRLYTRLMAENGNWLVEVKPWYVVGNTDDNPDITKYMGYYQLK
                     IGYHLGDAVLSAKGQYNWNTGYGGAELGLSYPITRHVRLYTQVYSGYGESLIDYNFNQ
                     TRVGVGVMLNDLF"
     gene            12455..12922
                     /gene="yigI"
                     /locus_tag="ACHKBL_003264"
     CDS             12455..12922
                     /gene="yigI"
                     /locus_tag="ACHKBL_003264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase YigI"
                     /protein_id="HFO5820096.1"
                     /translation="MSAVLTAEQALKLVGEMFVYHMPFNRALGMELERYEKEFAQLAF
                     KNQPMMVGNWAQSILHGGVIASALDVAAGLVCVGSTLTRHETISEDELRQRLSRMGTI
                     DLRVDYLRPGRGERFTATSSLLRAGNKVAVARVELHNEEQLYIASATATYMVG"
     gene            12974..>13843
                     /gene="rarD"
                     /locus_tag="ACHKBL_003265"
     CDS             12974..>13843
                     /gene="rarD"
                     /locus_tag="ACHKBL_003265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HFO5820097.1"
                     /translation="MDTKQTRQGVLLALAAYFIWGIAPAYFKLIYYVPADEILTHRVI
                     WSFFFMVVLMSICRQWSYLKTLIQTPKKIFMLAVSAVLIGGNWLLFIWAVNNHHMLEA
                     SLGYFINPLVNIVLGMIFLGERFRRMQWLAVILAICGVLVQLWTFGSLPIIALGLAFS
                     FAFYGLVRKKIAVEAQTGMLIETMWLLPVAAIYLFAIADSSTSHMGQNPMSLNLLLIA
                     AGIVTTVPLLCFTAAATRLRLSTLGFFQYIGPTLMFLLAVTFYGEKPGADKMVTFTFI
                     WVALAIFVMDAIYT"
BASE COUNT         3473 a         3596 c         3436 g         3338 t
ORIGIN      
        1 tcccttttgg gcgtaaataa aggtaaaaaa taacaatact atttttcttt cacaaaagaa
       61 ttaacaaaag gtttttcaag gatgagtgat attatttacc tgaccataac cggagaacaa
      121 caaggatgta tttcctctcg ttgcggaacg agcgcatcaa taggtaatcg ctggcagatt
      181 ggccatgaag atgaaatctt cgcgttctcg ctatctaata gtattacgaa tacaggcaaa
      241 gggtcacaat tacatggcct tagtttctgt aagttgattg acaaaagctc cccgttactt
      301 attaacgcta ttaacaataa cgagcagttg ttcatggagt ttgacttcta tcggatcaat
      361 agatttggtc gatgggagaa atattacaac attcaactcc gcggcgcgct cttatcagca
      421 atcaatcacc tgtttactga gaacaatctg gatacagaaa ccatcactgt cagttatgaa
      481 tatatactct ccaggcatct gattgctaat actgaattta gtcatttggc atttcccgat
      541 aactacaacc gtctgtttat accacggcaa aaaactaaag atgataatgg cctcaaaaca
      601 ctcaatagta aagcggttgg cagactgctt gctgctggcg gcatatacaa tgggaatatc
      661 gaaggattca gggaaacagc aaacaagttg ggaggagatg cacctgctgg ttacgatcaa
      721 gttatggata acaaagggtt actcatcgct ggtgcttcaa tagttgctgg agttgttctg
      781 ggaagaatga gattccctga tttggaatca tttgagcatt tcggagccag aggagcagta
      841 tcaggaagac catttaagcc cgaccttgcc ggtggtccaa tagaaaacct gacgatagat
      901 ggcgtaacaa tcaataggga aggtatcgct attgttgaaa agcatatctc cagatttgat
      961 catgatccag ctaatgatgt aatgatttca aggctaaaaa aaatagcaaa taaagaattg
     1021 cttcctgaaa aatacgatct gaactactat actcatgaat gccgcgagta tcagcgttat
     1081 tgtaatttag gatgggaaac aggtgaaccg aatggtttag atggatatga attatggaat
     1141 aatgcacata ctgcaactct agaagacttt aaaattaaag atactgactt atttcatccg
     1201 gatgcaaaaa aatgacgatt acgatagttg aatttaacgt gctgaaagtt atggctgaga
     1261 aagatattga ctggagctgg atggtgctgg atcgtacttt agctatacga aatattccag
     1321 gatttggcaa cgtagcgaat atcgtaacaa agctggttaa tcatgggttg gttgatatcg
     1381 taaatggtga gggcaactct aaaccacgct acagagtttc tcaatatgga ttgaatctta
     1441 tcaaagaaca acaagacaac ctcttctgat ccagccatta caggagcatg atttacattg
     1501 tgatctttca ataagatact catcaatttt ggtacagaat atcgtgatca ctaaaaatga
     1561 acttaacgtg ctgaacgtta tgactgagaa agatataaac tggaactgga tgaatctcga
     1621 tcgcacacta tccataaggc aaataccagg attcggtaat gttgttaata ttgttaacaa
     1681 attagctaac gaaggtttgg ttattattga ggatagcggg cataagtcta tgcctcacta
     1741 ccacgttagt gaaaagggat acaatctcgt gcaacgtgag aataaataac gtagcgttga
     1801 ttactgaacg gaatcactca aaactgtgtt ttgcgaaaaa aacgattcgg ttatttctac
     1861 tcctgaagat ctgcttcaat ctggcactca tgcaaaatgg cttccagtac tggaagccat
     1921 tatcgtactg caatttacaa tgactcgtta atactcatca tccccgacgc aaattctgcg
     1981 ccgcctgcac catgttcgcc agtgccgcgc gggtttctgg ccagccgcgc gttttcaggc
     2041 cacagtccgg gtttacccac agacgctctg ccggaatgcg ttttgccgct ttcttcagca
     2101 gggcttcaat ccattccacg ctcggtacgt ttggcgagtg aatgtcatag acgcctggac
     2161 caatttcatt tggataatca aactcttcaa acgactccag caactccatg tcggaacgcg
     2221 aggtttcgat ggtgatgacg tctgcgtcca gcgccgcaat cgaatccatg atgtcgttga
     2281 actcgcagta acacatgtga gtgtggattt gtgtgtcatc tttcgccacg gcggcgttga
     2341 tacggaaagc ctccacgccc cactggagat aggcatccca gtcgctgcga cgcagcggta
     2401 aaccttcgcg cagtgccggt tcatcaatct ggatgatgcc aattccagcg gcttccaggt
     2461 ccgccacttc atcacgcagc gccagcgcaa tttgtttggc gatggtttca cggctgacat
     2521 cttcacgcgg gaatgaccag cagagtatgg ttaccggccc cgtcagcatc cctttcaccg
     2581 gtttgtcggt cagcgattgt gcatatttcg cccactccac ggtaattggt gccgggcggc
     2641 taacgtcacc aatgacaatc ggtggcttca cgcagcggga accgtagctc tgtacccaac
     2701 cgttttgcgt aaagacaaag ccatccagat gctcgccaaa gtattccacc atgtcattac
     2761 gctcggcctc gccatgtacc agcacatcca gtcccaaacg ttcctgctcg acaatggcct
     2821 gcttgatatg ttccgcaatg cccgtgcggt agttgttggc gtcgagatta ccctttttga
     2881 aatccagacg cagtgtacga atttccgtgg tttgcgggaa ggaaccaatc gtggtagtcg
     2941 gccacgcggg cagtttaaaa cgcgcacgct gggcttcagc acgcacttca tagacattcg
     3001 cacgctgact gtcctgggcg gtaatggctg caagacgctt ttctaccgcc ggattatgga
     3061 cacgggtaga gtgacgacgc gcctgaatcg gagcgctcca ttctgccaga gccgccgtat
     3121 caccactgtt cagcgcatcg cgcagcagtg ccagttcatg gcatttttgt agggcgaagg
     3181 caaaccagct tttcacttct gcatcaagac gcgtttccac gctcaggtcg atggggctgt
     3241 gcagcaacga gcaggaagat gccacccaca aatcgcgttt gccgacaatg tccttaattt
     3301 gcgcatattt ctcggtaaga tcggcgcgcc agacgttacg accattgatc agacccgcag
     3361 acagtagcca gtcagaaggc agacgcttgt gcagttcagc aacgtcatct ttaccatgta
     3421 caaggtccac atgcagcccc tgaacaggca gcgcagtaat cgtgtcgaga tttggcgtta
     3481 cgccttcaaa ataggtagtc agcagcagtt tcacctggcc ctggagcgcg tcgtaagcgg
     3541 gtttgtatgc gtccagccac gcctgcggta gttccagtac cagcgctggt tcgtcgatct
     3601 gtacccactc aatgccgcgt tttgccagtt ctgccaacac ttgctgataa accggcagaa
     3661 tgtcgttcag caggctcagg cgatcaaact gttcaccttt cactttaccc agccacaggt
     3721 aggtaaccgg ccccagcagc acgggcttga ccttgtggcc cagcgccagc gcctcgtcca
     3781 cttcctccag cagctgcgtc caggtcagtt tgaactgttg gcctttaacg aactccggca
     3841 ccatgtagtg atagttggtg ttaaaccatt tggtcatttc cgctgccgcc gcaggttcgc
     3901 cagtcggtgc acgtccacga ccaatacgga acagggtgtc gatatctacc gaaccatctt
     3961 tgttctgatg acgtggcgga acgttaccca gcagcagact ggtggtcagt acatgatcgt
     4021 accaggcaaa atcgcccacc ggcagcaggt cgatacccgc ctgcttttgt tgatcccagt
     4081 gacgggcacg taattcacgc ccaacagcca gcagttcttc acgcgtggag ttccccgccc
     4141 aataactctc ttgcgctttt ttcagctcgc gacgcaggcc aacgcgaggg aaaccgaggg
     4201 tgtgattaag aattgtcatt atatgcccct tatggaattt ttagtattta gccgtccaga
     4261 tgtttacaca tccataattg gcggttactg tatattcctc aagcgcaaat tattcatgcc
     4321 gaagtgaagg actttcatga tcgaagtaaa acacctgaaa acgctacaag cgttgcggaa
     4381 ctgcggctca ctcgcagccg ctgcggcgac gttgcatcaa acgcaatccg ccctgtctca
     4441 ccagtttagc gatctggaac aacgccttgg cttccggcta tttgtgcgta aaagccagcc
     4501 gcttcggttt acaccgcagg gagaaatcct gttgcaactg gcgaatcagg tgctgcctca
     4561 aatcagccag gcactgcaag cctgcaatga accgcagcag acgcgcctgc gcattgccat
     4621 tgagtgccat agctgtattc agtggctgac gcccgcgtta gaaaatttcc ataagaactg
     4681 gccgcaggtg gagatggatt ttaaatcggg cgtgacgttt gacccgcagc ccgcgttgca
     4741 gcagggtgag ctggatctgg tgatgacctc cgatattctg ccgcgcagtg gcctgcatta
     4801 ttcgccgatg ttcgattatg aagtgcgtct ggtgttggca cctgaccatc cactggcggc
     4861 gaaaacgcga atcacgccgg aagacctcgc cagcgaaacg ctgttgattt atccggtgca
     4921 gcgtagccga ctggatgtct ggcggcattt tcttcagccc gcaggcgtta gcccgtcact
     4981 gaaaagcgtc gataacacct tgttgttgat tcagatggtt gccgcgcgga tgggcatcgc
     5041 tgcgctaccg cattgggtgg tggagagttt tgaacgccag ggtctggtgg tcaccaaaac
     5101 cctgggcgaa ggcttgtgga gccgactgta cgccgccgtg cgcgatggcg agcagcgtca
     5161 gccgattaca gaagcgttta ttcgttcagc gcgcaatcac gcctgcgatc atctgccgtt
     5221 tgtgaagagc gcggagcgac ccacttacga tgcacccaca gtgaggccag gatcaccagc
     5281 gcgcctgtaa taaacgttgg ccagtgcggc tgttggtgcc agatagccag gttaaccaac
     5341 agccctgccg gaacgtgcat attattcata atgcccagcg ttccggcgtc cacctgagtc
     5401 gcgccgtagt tccacataaa gtagccaata ccggaagcca ccacgccaag aaacaccaga
     5461 atgccccatt gcagcgtggt ttgcggcatt ttctgcgcat ttcccagcaa aaaccatgca
     5521 atcaccgcca ccaggaacgc accaagatag aaccacgcaa aggcgttatg ctgtggcatc
     5581 ggacgagttt ccatcaggcg tttgtaaccc accatgccga tggcaaaagt gatattggag
     5641 agttgcacca gcagcaggcc agtccagaag tggtcggtga cctgatcata gcgaataatt
     5701 ccggcaccaa tcaccgccaa caaggcgcta aaagcatagc cccagcgcag acggcgctta
     5761 ctcatgatgt cataaatcag cgtgatgtag agcggagtca gtacggtgaa cagcaacagt
     5821 tcggagaccg tcaggtagag ataggcgcga aaactcagca tatacatcac accgagctgc
     5881 atcgcgccca ccagcatata caggccaacc gttttcaggc tattgccacg ggtacgcaga
     5941 aacggcagaa acacgagtgc cgccaggcca acgcgcacca gcactgcaaa atagctatcg
     6001 acgtgccccg caaggtactc gccataaaag ctaaaggaga aggcccacag aatcgtggtg
     6061 atgataagta gcgccacaat ggaaaactct ttgattaacg agtatccatt gtagcgaagt
     6121 atcaggttga caactgacca aataaagaac gattacgata aatagagttt acgcagataa
     6181 tgcggcaccg cgtcttcggc attagtacca atcacttcca gctccggatg cagatctttc
     6241 agacgctggt gcgcactgcc cataatgcag cctttacctg ccatcgacag catttcggcg
     6301 tcgttcatcc cgtcaccaaa ggcaatacaa tctttcagac tgtagcccag tttcttcgcc
     6361 accgcttcca gggcatggcc ttttgaaacg ccgcccgcca tcacttccag acaagttagg
     6421 gtagagaagc tgacgttgac gcgatcgccc caacgagcgt tgattgcctg ctccagcggc
     6481 agcagttgct catgagaatc gcaggtgaag aacactttgc tgacgccttc cggctccagt
     6541 aatcctggct catacagcgc atatttgaac actgcttctt taaaaaagcg catctcttcc
     6601 gggcggtggc gattcataaa ccattcgtcg tcgcgataaa cgttagtaat gatgtccggg
     6661 ttgtcgttga ccacgccaaa cagatcgctg gcaatgtcgc gatccaggtt atgagcaaaa
     6721 atcagattgc catccagatc gtgaacacgc gcgccattgg aggtaatcat gtaagactta
     6781 atctccagat tatcgcgaat ttgccctaca tcaacgtggt ggcgaccggt cgcaaacaca
     6841 aagttaatgc cgcgcgcggt gagcagcttc agagtttctt tggcgtaagg ggataacgta
     6901 tggtcgggag aaagtaacgt gccatctaaa tcagacgcaa caacctggta cataagaaat
     6961 ttaaacctct gtagagcggt ttccgctggg tgagttatgc ctgttgaaaa aatcaacaat
     7021 ggcgtggagc gcgactgagc gcattgcgtc cttttcaaaa aggatctcat ggtacgcacc
     7081 tttaattacc agcggtcgtc ccccttcgac aggatggccc gcagcggtgc ggagttcaca
     7141 aaaacggtcg tgcatgcggt tatccaccac gcgttcctct tcagcctgca agagaagcgt
     7201 tggcgtggcg tcatcacccg caccggctaa cacctgttcg ccagccagaa tactttcgcg
     7261 tacccaatgg taggtcggcc caccgacgcg aatcgttggg tcatcagcat aaaagcgtaa
     7321 gttacgtcga tatcgctgtc tgctgtgggt cagtacgttg atagcaaacg gcaacgcgcg
     7381 ccagcgcccg gtgcctattg catagccatc acggaaacgt ggatgcgctt cggcccagtt
     7441 aaggatctgc cgcgccataa atgacggcat acgaatcacg atcccaaaca ttggcgcagt
     7501 tagcgcaatg gcgtcacata cacctggatg gcgttgcaga aataacgtgg agatcgcacc
     7561 gcccatcgaa tgtgccagta tatagcgctt acgccacgga ccaggctgga cctcctgttg
     7621 ccagaatgcc gccaggtcat cgacataatc attaaagcga ttaacatgcc caagatgcgg
     7681 atcggctaac aggcgaccgg aacgtccctg cccgcgatgg tcgatgatta agacatcaaa
     7741 ccccaaatgg aacaggtcgt aggccagttc cgcatatttt acgtagctct caatacgccc
     7801 cgggcagatg actatcaccc ggtcatggtg ctgtgcgcga aaacggacaa agcgcaccgg
     7861 aatgtcatct acaccagtaa actctgcttc atcacgctga cgccagaaat cagtcagcgg
     7921 tcccatggta aaagcagcaa acgcgttttc tcttgtttcc cagtcttttt gctgctgaaa
     7981 catcgggtaa tctgcctctt aaaccacgta aaatcgtttt tttaagcgtg cctgacacaa
     8041 cgcttcgaca gtagcgtatt gtggcacaaa aatagacaca ccgggagttc atcatgacct
     8101 tagaatggtg gtttgcctac ctgctgacat cgatcatttt aagcctgtcg ccaggctctg
     8161 gtgcaatcaa caccatgacc acctcgctca accacggtta tcgcggcgcg gtggcgtcta
     8221 ttgctgggct tcagaccgga ctggcgattc atattgtgct ggttggcgtg gggttgggga
     8281 cgctattttc ccgctcagtg attgcgtttg aagtgttgaa gtgggcaggc gcagcttact
     8341 tgatttggct tgggatccag cagtggcgcg ccgctggcgc aattgatctt aaaccgctgg
     8401 cctctactca atcgcgtcga catttgttcc agcgcgcagt ttttgtcaat ctcaccaatc
     8461 ccaaaagtat tgtgtttctg gcggcgctat ttccgcaatt catcatgccg cagcagccac
     8521 aactgatgca gtatatcgtg ctcggcgtca ccactattgt ggtcgatatt attgtgatga
     8581 tcggttacgc cacacttgct caacggattg cgttatggat aaaagggccg aaacagatga
     8641 aggcattgaa taagattttt ggctcgctgt ttatgctggt aggagcgttg ttggcatcgg
     8701 cgaggcatgc gtgaaaaata atgtcggatg cggcataaac gccttatccg acttactctg
     8761 atgaagcgtt tggcatcaac gagaaataat caaatgaatg ccaaatccgg caaataacgc
     8821 cccggcaaaa ccatcaatcc acttcgccag acgttgataa ccacggcgca tttgcggtag
     8881 agcaaacagg ctggcaacca ccgtaaacca tgccagtgtt tcgataataa tcaacgcgaa
     8941 aatgccccag cgctcagtgg tgccgacgtt atcgccgaca aacagcgaga ataccgaacc
     9001 aaagtagata attgctttcg gattggcgag attggtcagt aagcctttca ggaaactgcg
     9061 cccgcttttt gccagttcta cctgtggcgc aggcgcagaa acgacctctt ttttcagtgc
     9121 gctacgcagc atctgataac ccatccagca gagatacagg ccaccgccca ccataatcag
     9181 cgtatgcagc caggccattt tttcgataat caaatgcagg ccaagcaacg cgatccctgc
     9241 ccagaccatt acgccgcagg taatgcccag cacgcccatc atcgcctctt tacgggaacg
     9301 actgacagcg gtctgagaaa caaagaagaa atcgggaccg gggctcataa gcgccacaat
     9361 gtgcaccatg gcaacggtga ggaataacat caacattcaa ttgactcgcg gagaaatcgt
     9421 ttacggagtc ttcatcctgg cactttttta tgctgctgac tactcttcgt catcgccatc
     9481 gacatgcgca cgaattagcg ccataaacgg tttgccaaag cgttccagct tgcgcatccc
     9541 aacgccgtta acgctgagca tttcgctggc ggagatcggc atctgttccg ccatctcaat
     9601 caaggtcgca tcgttaaaca ccacatacgg cgggacatta ctttcatcgg caatcgattt
     9661 acgcagtttg cgtaatttgg cgaacagttt gcgatcgtaa ttgccaccga aagatttctg
     9721 cattgctttc ggtttgagcg ccacaatacg cggcacggca agttgtaaag aggattcgcc
     9781 gcgcaacacc gggcgtgcgg cttctgtcag ttgtaatgcc gaatgctggg cgatgttttg
     9841 cgtcaccaga ccgagatgaa tcaactggcg gattacgctc acccagtgct catgactttt
     9901 atcgcggccc atgccgtaga ctttcagttt gtcatgacca tagtcgcgga ttcgctggtt
     9961 attggcgccg cgaatcactt ccactacata gcccatccca aaacgctgat tcacacgacc
    10021 aatggtggaa agcgcaatct gcgcatcggt tgaaccatcg tattgtttcg gcggatcgag
    10081 gcagatatcg cagttcccgc acggctcctg ccgcccttcg ccaaagtagt tcagcagcac
    10141 cagacgacgg caagtttgcg cttcggcaaa agcgcccatc gcattgagtt tatggcgctc
    10201 gatatcctgc aactgcccct gcggcttctc ttccagacaa cggcgcagcc acgccatatc
    10261 agccggatcg taaaacagca tcgcttccgc gggcaggcca tcacgcccgg cgcgaccggt
    10321 ttcctgataa taggattcga tattgcgtgg aatatcaaag tgaaccacga agcggacgtt
    10381 aggcttgttg atgcccatgc cgaacgctac cgtcgccacc acaatttgca ggtcatcacg
    10441 ctggaatttt tcctgcacgt ccgcacggac atcgttttca agcccggcgt gataagccgc
    10501 tgcgctgata cccttgcttt gcagacgcgc ggcggtgtct tctactttcg cacgactatt
    10561 acagtaaata atccccgact taccgcgttg ttcctgtaca taacgcatca actgatcgag
    10621 cggtttgaat ttctccatca gcatgtagcg aatattcgga cggtcaaaac tgctaatttg
    10681 aatcagcgga tcgttcagcc ccagcaggcg cacgatatcc tgccgcgtgg tgtcgtcggc
    10741 ggtggcggtt agcgccataa acggcagcgt cgggaaccgc tggcgcaact gaccgagcgc
    10801 ggcatattcc gggcggaaat cgtggcccca ttgggagata cagtgcgctt catcaacggc
    10861 taataacacc ggattccagt gcgccagatg ctcaagaaag ttatccagca tcaggcgttc
    10921 cggggcgata taaagcagac gaatttgccc ggtgcgacag cctgtcatca cttcaagctg
    10981 ctgctcgcgg gtttgcgtcg agttaaggca cgccgccgcc acaccgttgg cctgtagctg
    11041 atccacctga tctttcatca acgaaatcag tggtgaaaca accacggtaa gaccgtttag
    11101 cagtaaggca ggaatttgat agcaaaggga ttttccgcca ccagtgggca tgacgaccag
    11161 gcaatcgcga ccggaaagta cggtgtcgat aatctcttcc tggccggggc gaaactgttg
    11221 gtagccaaag gtttcttgta aaacctgttt agctccggac tccagattca acacttccgc
    11281 ctgcgccaca ttaaccccga ttgcctgaaa taaaaacagg cgctattttc agcgcctgcg
    11341 cgagaaactg caatgctttt agtctctgag agggtaatcc tggatactgg attccagttt
    11401 tgcctttcaa tcaactcaca ttagaacaag tcgttcagca taacccccac acccacacgg
    11461 gtctggttaa agttatagtc gatgagcgat tcgccataac cgctgtaaac ctgggtataa
    11521 aggcgcacat gcctggtgat cgggtaactt aagcctaact ccgcaccacc gtaaccggtg
    11581 ttccagttgt actgtccttt cgcactcagc accgcatcac ccaggtgata gccgatttta
    11641 agctggtagt aacccatata tttggtgata tctgggttat cgtcagtatt ccccaccaca
    11701 taccacggct tcacctctac cagccagtta ccgttttctg ccatcagacg ggtataaagg
    11761 cggttccagc tgcgggaagt cgggtctgaa cgcccgttag agtcgtggtt ataacccatc
    11821 tccacatcgc gcagcgtcca gccagcaaag cggtaatcgg tggcaaaacc aaggaacaat
    11881 tgcggttcgt agttggtttc acgaaacggt gaagactctt cgctattgga cagttgccac
    11941 caggattttt gcgtgtaaga agcccccaac accgagtttg gccccaatat tccgcgccac
    12001 agcggaaatg ccaggctcaa ctggaatttc acttcatcct tacgcgcgtt ttccgcccag
    12061 tcataactgg cgatcgcttc tttgttcaga tcgctggttt gggtgtagat gaggtagttg
    12121 gtgtcataag gatagagcgt gaaaggattg tcatgctcct gcagcatatt cgcaataata
    12181 ctgccacgca ccgcaggcgc gtcatgcacc tcttttaccg ttgcctcttg cgcatacact
    12241 gccattggca acataaacac cggcaacaac cagccctgca gagtccgcat cggtcgtgtt
    12301 ctccgtaacg aattattgat tagatgaata tttttctgcg ggctatttta catacttaac
    12361 tcctttgccg ttagttatcc cttcctaaag tagaaaacca caattaagaa gcataaaatc
    12421 aacatttcat taaccaatga ggtgtaagga tgatatgtct gccgtactga ccgctgaaca
    12481 agccctgaaa ttagtgggtg agatgtttgt ttatcacatg ccatttaacc gcgcattggg
    12541 gatggaactg gagcgttacg aaaaagagtt cgcacagctg gcctttaaaa atcagccaat
    12601 gatggtgggt aactgggcgc aaagcatttt gcacggcggg gtcattgcgt cggcgctgga
    12661 tgtcgccgca ggtctggtgt gcgtgggaag taccttaacc cgccacgaaa ccatcagtga
    12721 agatgaacta cgccagcggc tatcgcggat ggggaccatt gatcttcgcg tcgattatct
    12781 gcgcccaggc aggggcgagc gttttactgc taccagtagt ctgttgcgtg caggcaataa
    12841 agtcgccgtt gcccgcgttg aattacacaa tgaagaacag ctttatatag ccagtgccac
    12901 cgccacctat atggtaggtt gataacgtaa aagcaggtaa actgctttta ccaataaaaa
    12961 atgagttttg ccaatggaca ccaaacaaac gcggcagggc gtattactcg ctcttgccgc
    13021 ttattttatt tggggtattg cgccagcgta cttcaagctg atttactacg tgcccgccga
    13081 tgagatcctg acgcatcgcg tgatctggtc gtttttcttt atggtggtgc tgatgagcat
    13141 ttgccgccag tggtcctatt taaaaacgct gattcagaca ccaaagaaaa tcttcatgct
    13201 ggcagtctct gccgtgctga ttggtggcaa ctggctactg tttatctggg cggtgaacaa
    13261 tcaccatatg ctggaagcga gccttggtta ctttattaac ccgctggtga acattgtgct
    13321 ggggatgatt ttcctcggcg agcgattccg ccggatgcaa tggctggcgg tgattctggc
    13381 gatatgtggt gtattggtcc agctctggac ttttggttcg ctgccaatca tcgcgctggg
    13441 gctggcgttt agttttgcct tctacggcct ggtacgcaag aaaattgccg ttgaagcgca
    13501 aaccggcatg ttaatcgaaa ccatgtggct gctgcccgtg gcggcgattt acctgtttgc
    13561 tattgccgac agctcaacca gccatatggg gcaaaacccg atgtcgctga atttactgct
    13621 gatcgccgcc ggtattgtca ccaccgtgcc actgttatgt tttaccgcag cagctacacg
    13681 cttacgactc tcaacattag gctttttcca gtacattggg ccgacgctaa tgttcctgtt
    13741 ggcagtgacg ttttatggtg aaaaaccggg tgccgataag atggtgactt tcacctttat
    13801 ttgggtggcg ctggcaattt ttgtgatgga tgcgatttat acg
//
LOCUS       DAZFLD010000131        13495 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.132, whole genome shotgun sequence.
ACCESSION   DAZFLD010000131 DAZFLD010000000
VERSION     DAZFLD010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13495
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.132"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            137..1246
                     /gene="livK"
                     /locus_tag="ACHKBL_003266"
     CDS             137..1246
                     /gene="livK"
                     /locus_tag="ACHKBL_003266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter substrate-binding protein LivK"
                     /protein_id="HFO5820098.1"
                     /translation="MKRNAKTIIAGMIALAISHTAMADDIKVAVVGAMSGPIAQWGDM
                     EFNGARQAIKDINAKGGIKGDKLVGVEYDDACDPKQAVAVANKIVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMISPGATNPELTQRGYQYIMRTAGLDSSQGPTAAKYILETVK
                     PQRIAIIHDKQQYGEGLARSVQDGLKAANANVVFFDGITAGEKDFSALIARLKKENID
                     FVYYGGYYPEMGQMLRQARSVGLKTQFMGPEGVGNASLSNIAGDAAEGMLVTMPKRYD
                     QDPANKGIVDALKADKKDPSGPYVWITYAAVQSLATALERTGSDEPLALVKDLKANGA
                     NTVIGPLNWDEKGDLKGFDFGVFQWHADGSSTAAK"
     gene            1294..2220
                     /gene="livH"
                     /locus_tag="ACHKBL_003267"
     CDS             1294..2220
                     /gene="livH"
                     /locus_tag="ACHKBL_003267"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivH"
                     /protein_id="HFO5820099.1"
                     /translation="MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHG
                     EVYMIGSYVSFMIIAALMMMGIDTGWLLVAAGFVGAIVIASAYGWSIERVAYRPVRNS
                     KRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVI
                     WIVTFLAMLALTIFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAV
                     AGVLLGQFYGVINPYIGFMAGMKAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLS
                     TEYKDVVSFALLILVLLVMPTGILGRPEVEKV"
     gene            2217..3494
                     /gene="livM"
                     /locus_tag="ACHKBL_003268"
     CDS             2217..3494
                     /gene="livM"
                     /locus_tag="ACHKBL_003268"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     permease LivM"
                     /protein_id="HFO5820100.1"
                     /translation="MKPMHIAMALLSAAMFFVLAGVFMGVQLELDGTKLVVDTASEIR
                     WQWVFIGTAVVFFFQLVRPAFQKGLKSVSGPKFILPAIDGSTVKQKLFLVALLVLAVA
                     WPFMVSRGTVDIATLTMIYIILGLGLNVVVGLSGLLVLGYGSFYAIGAYTFALLNHYY
                     GLGFWTCLPIAGLMAAAAGFLLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGG
                     PNGISQIPKPTLFGLEFSRTAREGGWDTFSNFFGLKYDPSDRVIFLYLVALLLVVLSL
                     FVINRLLRMPLGRAWEALREDEIACRSLGLSPRRIKLTAFTISAAFAGFAGTLFAARQ
                     GFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAILLVVSRELMRDFNEYSMLMLGG
                     LMVLMMIWRPQGLLPMTRPQLKLKNGAAKGEQA"
     gene            3491..4258
                     /gene="livG"
                     /locus_tag="ACHKBL_003269"
     CDS             3491..4258
                     /gene="livG"
                     /locus_tag="ACHKBL_003269"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HFO5820101.1"
                     /translation="MSQPLLSVNGLMMRFGGLLAVNNVNLELYPQEIVSLIGPNGAGK
                     TTVFNCLTGFYKPTGGTILLRDQHLEGLPGQQIARMGVVRTFQHVRLFREMTVIENLL
                     VAQHQQLKTGLFSGLLKTPSFRRAQSEALDRAATWLERIGLLEHANRQASNLAYGDQR
                     RLEIARCMVTQPEILMLDEPAAGLNPKETKELDELIAELRNHHNTTILLIEHDMKLVM
                     GISDRIYVVNQGTPLANGTPEQIRNNPDVIRAYLGEA"
     gene            4260..4973
                     /gene="livF"
                     /locus_tag="ACHKBL_003270"
     CDS             4260..4973
                     /gene="livF"
                     /locus_tag="ACHKBL_003270"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivF"
                     /protein_id="HFO5820102.1"
                     /translation="MEKVMLSFDKVSAHYGKIQALHEVSLHINQGEIVTLIGANGAGK
                     TTLLGTLCGDPRATSGRIVFDDKDITDWQTAKIMREAVAIVPEGRRVFSRMTVEENLA
                     MGGFFAERDQFQERIKWVYELFPRLHERRVQRAGTMSGGEQQMLAIGRALMSNPRLLL
                     LDEPSLGLAPIIIQQIFDTIEQLREQGMTIFLVEQNANQALKLADRGYVLENGHVVLS
                     DTGDALLANEAVRSAYLGG"
     gene            5061..5282
                     /locus_tag="ACHKBL_003271"
     CDS             5061..5282
                     /locus_tag="ACHKBL_003271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFO5820103.1"
                     /translation="MHTMNYSEARQNLALVLETAATGTPVTITRRGHKAAVIISAEEF
                     ERYQKAKLDAEFDAIMAIHGSEIKELADK"
     gene            5279..5656
                     /locus_tag="ACHKBL_003272"
     CDS             5279..5656
                     /locus_tag="ACHKBL_003272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HFO5820104.1"
                     /translation="MTIQFISAEEIIYFHDKLLSVTPGVTGMSDPGRAEALLYRVLNK
                     YEYEGVSDLWLLAAMHLLAISRGHIFNDGNKRTALFITLLFLKRNGIILAANPEFVEL
                     TVDVAAGKVSLEDIVTRLRQRGT"
     gene            5865..7181
                     /gene="ugpB"
                     /locus_tag="ACHKBL_003273"
     CDS             5865..7181
                     /gene="ugpB"
                     /locus_tag="ACHKBL_003273"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
                     /protein_id="HFO5820105.1"
                     /translation="MKPLHYTASALALGLALMGNAQAVTTIPFWHSMEGELGKEVDSL
                     AQRFNAENPDYKIVPTYKGNYEQNLSAGIAAFRTGNAPAILQVYEVGTATMMASKAIK
                     PVYDVFKEAGIQFDESQFVPTVSGYYSDSKTGHLLSQPFNSSTPVLYYNKDAFKKAGL
                     DPEQPPKTWQDLADYAAKLKASGMKCGYASGWQGWIQLENFSAWNGLPFASKNNGFDG
                     TDAVLEFNKPEQVKHIAMLEEMNKKGDFSYVGRKDESTEKFYNGDCAMTTASSGSLAN
                     IREYAKFNYGVGMMPYDADAKDAPQNAIIGGASLWVMQGKDKETYTGVAKFLDFLAKP
                     ENAAEWHQKTGYLPITKAAYDLTREQGFYEKNPGADTATRQMLNKPPLPFTKGLRLGN
                     MPQIRVIVDEELESVWTGKKTPQQALDTAVERGNQLLRRFEQSTKS"
     gene            7357..8244
                     /gene="ugpA"
                     /locus_tag="ACHKBL_003274"
     CDS             7357..8244
                     /gene="ugpA"
                     /locus_tag="ACHKBL_003274"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HFO5820106.1"
                     /translation="MSSSRPVFRSRWLPYLLVAPQLIITVIFFIWPAGEALWYSLQSV
                     DPFGFSSQFVGLDNFVTLFHDSYYLDSFWTTIKFSTFVTVSGLLVSLFFAALVEYIVR
                     GSRFYQTLMLLPYAVAPAVAAVLWIFLFNPGRGLITHFLAEFGYDWNHAQNSGQAMFL
                     VVFASVWKQISYNFLFFYAALQSIPRSLIEAAAIDGAGPIRRFFKIALPLIAPVSFFL
                     LVVNLVYAFFDTFPVIDAATSGGPVQATTTLIYKIYREGFTGLDLASSAAQSVVLMFL
                     VIVLTVVQFRYVESKVRYQ"
     gene            8241..9086
                     /gene="ugpE"
                     /locus_tag="ACHKBL_003275"
     CDS             8241..9086
                     /gene="ugpE"
                     /locus_tag="ACHKBL_003275"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HFO5820107.1"
                     /translation="MIENRPWLTIFSHTMLILGIAVILFPLYVAFVAATLDKQAVYAA
                     PMTLIPGTHLLENIHNIWVNGVGTNSAPFWRMLLNSFVMAFSITLGKITVSMLSAFAI
                     VWFRFPLRNLFFWMIFITLMLPVEVRIFPTVEVIANLKMLDSYAGLTLPLMASATATF
                     LFRQFFMTLPDELVEAARIDGASPMRFFCDIVFPLSKTNLAALFVITFIYGWNQYLWP
                     LLIITDVDLGTTVAGIKGMIATGEGTTEWNSVMAAMLLTLIPPVVIVLVMQRAFVRGL
                     VDSEK"
     gene            9088..10158
                     /gene="ugpC"
                     /locus_tag="ACHKBL_003276"
     CDS             9088..10158
                     /gene="ugpC"
                     /locus_tag="ACHKBL_003276"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol 3-phosphate ABC transporter ATP
                     binding protein UgpC"
                     /protein_id="HFO5820108.1"
                     /translation="MAGLKLQAVTKSWDGKTQVIKPLTLDVADGEFIVMVGPSGCGKS
                     TLLRMVAGLERVTTGDIWIDRKRVTEMEPKDRGIAMVFQNYALYPHMSVEENMAWGLK
                     IRGMGKQQIAERVKEAARILELDGLLKRRPRELSGGQRQRVAMGRAIVREPAVFLFDE
                     PLSNLDAKLRVQMRLELQQLHRRLKTTSLYVTHDQVEAMTLAQRVMVMNGGVAEQIGT
                     PVEVYEKPASLFVASFIGSPAMNLLKGRVNNEGTHFELDGGIALPLNGGYRQYAGRKM
                     TLGIRPEHITLRSQAEGGVPLVMDTLEILGADNLAHGRWGEQKLVLRLAHQERPTAGS
                     TLWLHLPENQLHLFDGETGQRV"
     gene            10155..10898
                     /gene="ugpQ"
                     /locus_tag="ACHKBL_003277"
     CDS             10155..10898
                     /gene="ugpQ"
                     /locus_tag="ACHKBL_003277"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFO5820109.1"
                     /translation="MSNWPYPRIVAHRGGGKLAPENTLAAIDVGARYGHKMIEFDAKL
                     SKDGEIFLLHDDNLERTSNGWGVAGELNWQDLLRVDAGSWYSKAFKGEPLPLLSQVAE
                     RCREHGMMANIEIKPTTGTGPLTGKTVALAARELWTGMTPPLLSSFEIDALEAAQQAA
                     PELPRGLLLDEWRDDWRELTARLGCVSIHLNHKLLDKARVMQLKDAGLRILVYTVNKP
                     QRAAELLRWGVDCICTDAIDVIGPNFTAQ"
     gene            complement(10885..11325)
                     /locus_tag="ACHKBL_003278"
     CDS             complement(10885..11325)
                     /locus_tag="ACHKBL_003278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2756 family protein"
                     /protein_id="HFO5820110.1"
                     /translation="MKRLLILTALLPFVGFAQPINTLNNPNQPGYQIPSQQRMQTQMQ
                     TQQIQQKGMLNQQLKTQTQLQQQHLENQINNNSQRVLQSQPGERNPARQQMLPNTNGG
                     MLNSNRNPDSSLNQQHMLPERRNGDMLNQPSTPQPDIPLKTIGP"
     gene            11445..13190
                     /gene="ggt"
                     /locus_tag="ACHKBL_003279"
     CDS             11445..13190
                     /gene="ggt"
                     /locus_tag="ACHKBL_003279"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036374 - glutathione hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006751 - glutathione catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HFO5820111.1"
                     /translation="MIKPTFLRRVAIAALFTGSCFSTVAAPPAPSPVSYGVEEDVFHP
                     VRAKQGMVASVDATATQVGVDILKEGGNAVDAAVAVGYALAVTHPQAGNLGGGGFMLI
                     RSKNGNTTAIDFREMAPAKATRDMFLDDQGNPDSKKSLTSHLASGTPGTVAGFSLALD
                     KYGTMPLNKVVQPAFKLARDGFIVNDALADDLKTYGSEVLPNHENSKAIFWKEGAPLK
                     KGDKLVQANLAKSLEMIAENGPDEFYKGTIAEQIAQEMQKNGGLITKEDLAAYKAVER
                     TPISGEYRGYQVYSMPPPSSGGIHIVQILNILENFDMQKYGFGSADAMQIMAEAEKYA
                     YADRSEYLGDPDFVKVPWQALTNKAYAKSIADQIDINKAKPSSEIRPGKLAPYESNQT
                     THYSVVDKDGNAVAVTYTLNTTFGTGIVAGESGILLNNQMDDFSAKPGVPNVYGLVGG
                     DANAVGPNKRPLSSMSPTIVVKDGKTWLVTGSPGGSRIITTVLQMVVNSIDYGMNVAE
                     ATNAPRFHHQWLPDELRVEKGFSPDTLKLLEAKGQKVALKEAMGSTQSIMVGPDGELY
                     GASDPRSVDDLTAGY"
     gene            complement(13228..>13495)
                     /locus_tag="ACHKBL_003280"
     CDS             complement(13228..>13495)
                     /locus_tag="ACHKBL_003280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YrhB family protein"
                     /protein_id="HFO5820112.1"
                     /translation="AFAKANHYLDDADLPVVITLHGRFIQGWYFCFESQEFLESGDEA
                     ARLAGNAPFIIDKDSGEIHSLGTAKPLEEYLQDYEIKKATFGLP"
BASE COUNT         3006 a         3405 c         3877 g         3207 t
ORIGIN      
        1 atcgctatca tcacaaaata ctgtgctaaa cccttaatca gacaggcaaa aacagtgcag
       61 tataaaaaaa gaacagtctg atttgttaac acataagaac aaagcaacac aacatcacga
      121 atggggattt ttgactatga aacggaatgc gaaaactatc atcgcaggga tgattgcact
      181 ggcaatttca cacaccgcta tggctgacga tattaaagtc gccgttgtcg gcgcgatgtc
      241 cggcccgatt gcccagtggg gcgatatgga atttaacggc gcgcgtcagg cgattaaaga
      301 cattaatgcc aaagggggaa ttaaaggcga taagctggtt ggcgtggaat atgacgacgc
      361 ctgcgacccg aaacaagccg ttgcggtcgc caacaaaatc gttaacgacg gcattaaata
      421 cgttattggg cacctgtgtt cctcctctac ccaacctgca tcagatatct acgaagacga
      481 aggtattctg atgatttcgc cgggagcgac caatccggag ctgacccaac gcggttatca
      541 atacatcatg cgtactgccg gtctggactc ttcccagggg ccaacggcgg caaaatacat
      601 tcttgagacg gtgaagcccc agcgcatcgc catcattcac gacaaacaac agtatggcga
      661 agggctggcg cgttcggtgc aggacggatt gaaagcagcc aatgccaacg tcgtcttctt
      721 cgacggcatt accgctggtg agaaagattt ctccgcgctg atcgcccgcc tgaaaaaaga
      781 aaacatcgac ttcgtttact acggcggtta ctacccggaa atggggcaga tgctgcgcca
      841 ggcccgttcc gttggcctga aaactcagtt tatggggccg gaaggggtgg gtaatgcgtc
      901 gctgtcgaac attgctggtg atgccgccga aggtatgttg gtcactatgc caaaacgcta
      961 tgaccaggac ccggcaaaca aaggcatcgt tgatgcgctg aaagcagaca agaaagatcc
     1021 gtccggacct tatgtctgga tcacctacgc ggcggtgcaa tctctggcga ctgcccttga
     1081 gcgtaccggc agcgatgagc cgctggcgct ggtgaaagat ttaaaagcta acggtgcaaa
     1141 caccgtgatt gggccgctga actgggatga aaaaggcgat cttaagggat ttgattttgg
     1201 tgtcttccag tggcacgccg acggttcatc gacggcagcc aagtgatcat cccaccgccc
     1261 gtgaaatgcg ggcggtttta gaaaggttac cttatgtctg agcagttttt gtatttcttg
     1321 cagcagatgt ttaacggcgt cacgctgggc agtacctacg cgctgatagc catcggctac
     1381 accatggttt acggcattat cggcatgatc aacttcgccc acggcgaggt ttatatgatt
     1441 ggtagctatg tctcctttat gatcatcgcc gcactgatga tgatgggcat tgataccggc
     1501 tggctgctgg tggcggcggg attcgtcggc gcaatcgtca ttgccagcgc ctacggctgg
     1561 agtatcgaac gggtagccta tcgtccggtg cgtaactcta agcgcctgat tgcgcttatt
     1621 tccgcaatcg gcatgtctat cttcctgcaa aactacgtca gcctgaccga aggttcgcgc
     1681 gacgtggcgc tgcccagcct gtttaacggt cagtgggtgg tgggccatag cgaaaacttc
     1741 tctgcctcta ttaccaccat gcaggcggtg atatggattg ttactttcct cgccatgctg
     1801 gcgctgacga ttttcattcg ctattcccgt atggggcgcg cctgccgtgc ctgcgcggaa
     1861 gatctgaaaa tggcgagcct gcttggcatc aacaccgacc gggtgattgc gctgaccttt
     1921 gtgattggcg cggcgatggc ggcagtggcg ggcgtgctgc tcggtcagtt ctacggcgta
     1981 attaaccctt acatcggctt tatggccggg atgaaagcct ttaccgctgc ggtgctcggt
     2041 gggattggca gcattccggg ggcgatgatt ggcgggctga tcctggggat tgcggaggcg
     2101 ctctcttctg cctatctgag tacggaatat aaagatgtgg tctcattcgc cctgctgatc
     2161 ctggtgctgc tggtgatgcc gaccggtatt ctgggtcgcc cggaggtaga gaaagtatga
     2221 aaccgatgca tattgcaatg gcgctgctct ctgccgctat gttctttgtg ctggcgggcg
     2281 tctttatggg cgtgcaactg gagctggatg gcaccaaact ggtggtcgac acggcttcag
     2341 agatccgttg gcagtgggtg tttatcggca cggcggtggt ctttttcttc cagcttgtgc
     2401 ggcctgcttt ccagaaaggg ctaaaaagcg tttccgggcc gaagtttatt ctgcccgcga
     2461 ttgatggctc cacggtgaag cagaaactgt tcctcgtggc gctgctggtg cttgcagtgg
     2521 cgtggccgtt tatggtgtcg cgtgggacgg tggatatcgc cactctgacc atgatctaca
     2581 ttattctcgg tctcgggctg aacgtggttg ttggtctttc tggtctgctg gtgctgggat
     2641 acggcagctt ttacgccatc ggcgcttaca cttttgcgct gctcaatcac tattacggtc
     2701 tgggcttctg gacctgtctg ccgattgccg ggttgatggc ggcggcggcg ggcttcctgc
     2761 tcggttttcc ggtgctgcgt ctgcgcggtg actatctggc gatcgttacc ctcgggttcg
     2821 gcgaaattgt gcgcatattg ctgctcaata acaccgaaat taccggcgga ccgaacggta
     2881 tcagtcagat tccgaaaccg acgctcttcg gactcgagtt cagccgtacc gcccgcgaag
     2941 gcggctggga cacgttcagt aatttctttg gcctgaaata cgatccctcc gatcgcgtca
     3001 tcttcctcta cctggtggcg ttgctgctgg tggtgttaag cctgtttgtc attaaccgcc
     3061 tgctgcggat gccgctgggc cgtgcgtggg aagcgttgcg tgaagacgag atcgcctgcc
     3121 gttcgctggg cttaagcccg cgtcgtatca agctgactgc ctttaccatc agcgccgcgt
     3181 ttgctggttt tgccggaacg ctgtttgctg cgcgtcaggg ctttgtcagc ccggaatcct
     3241 tcacttttgc cgaatcggcg tttgtgctgg cgatagtggt gctcggtggt atgggttcgc
     3301 aatttgcggt gattctggcg gcaattttgc tggtggtgtc gcgagagttg atgcgtgatt
     3361 tcaacgaata cagcatgtta atgctcggtg gtttgatggt gctgatgatg atctggcgtc
     3421 cgcagggctt gctgcccatg acgcgcccgc aactcaagct gaaaaacggc gcagcgaaag
     3481 gagagcaggc atgagtcagc cattattatc tgttaacggc ctgatgatgc gcttcggcgg
     3541 cctgctggcg gtgaataacg tcaatcttga actgtacccg caggagatcg tctcgttaat
     3601 cggccctaac ggtgccggaa aaaccacggt ttttaactgc ctgaccggtt tctacaaacc
     3661 caccggtggc actattttac tgcgcgatca gcatctggaa ggtttgcccg gccagcaaat
     3721 cgcccgcatg ggcgtggtgc gcaccttcca gcatgtgcgt ctgtttcgtg aaatgacggt
     3781 gattgaaaac ttgctggtgg cgcagcatca gcaactgaaa accgggctgt tctccggcct
     3841 gttgaaaacg ccttctttcc gccgcgccca gagcgaagcg ctcgaccgtg ccgcaacctg
     3901 gcttgagcgc attggtttgc tcgaacacgc taaccgtcag gcgagtaacc tggcctacgg
     3961 tgaccagcgc cgtctggaga tcgcccgctg catggtaacg cagccggaga ttttaatgct
     4021 cgacgaaccg gcagcaggtc ttaacccgaa agagacgaaa gagctggatg agctgattgc
     4081 cgaactgcgt aaccatcaca acaccactat cttgttgatt gaacacgata tgaagctggt
     4141 gatggggatt tcagacagaa tttacgtggt caatcagggg acgccgctgg caaacggtac
     4201 gccggagcag atccgtaata acccggacgt gatccgtgcc tatttaggtg aggcataaga
     4261 tggaaaaagt catgttgtcc tttgacaaag ttagcgccca ctacggcaaa atccaggcgc
     4321 tgcatgaggt gagcctgcat atcaatcagg gcgaaattgt cacgctgatt ggcgcgaacg
     4381 gggcggggaa aaccaccttg ctcggcacgt tatgcggcga cccacgcgcc accagcgggc
     4441 gaattgtgtt tgatgataaa gacattaccg actggcagac ggcgaaaatc atgcgcgaag
     4501 cggtggcgat tgtcccggaa ggacgtcgcg tcttctcacg catgacggtg gaagagaatc
     4561 tggcgatggg cggctttttt gctgaacgcg accagttcca ggagcgcata aagtgggtgt
     4621 atgaactgtt tccacgtctg catgaacgcc gtgttcagcg ggcaggcacc atgtccggcg
     4681 gtgaacagca gatgctggcg attggtcgcg ctttgatgag taacccacgt ttgttattgc
     4741 ttgatgaacc ctcgctcgga ctggcaccga ttatcatcca gcaaattttc gacaccatcg
     4801 agcaactgcg cgagcagggg atgaccatct tcctcgtcga gcagaacgcc aaccaggcgc
     4861 tgaagctcgc tgatcgcggc tacgtgctgg aaaacggtca tgtggtgctc tccgatactg
     4921 gcgatgcgct gctggcgaac gaagcggtga gaagtgcgta tttaggcggg taataatatt
     4981 ttgagtaaaa aggagtcgaa tgactccttt tttgtacttg ttagttgtac aatttaattg
     5041 tacttaatat ggaggatatc atgcatacca tgaactacag cgaagcgcgg cagaaccttg
     5101 cattagttct ggaaacagcg gcgacaggta ctccggtaac gatcacgagg cgtgggcata
     5161 aggcagctgt gattatcagc gcagaagagt ttgaacgtta ccagaaggct aaactggacg
     5221 cagaatttga cgccatcatg gctattcatg gcagcgaaat taaggagtta gcggataaat
     5281 gaccattcag tttatctcag cggaggagat aatttatttc cacgataaat tgcttagcgt
     5341 tacaccaggt gtaacaggaa tgtccgatcc agggcgagca gaagcgcttt tgtaccgggt
     5401 actgaataag tatgagtatg aaggcgtttc tgatctttgg ctgctggcgg cgatgcatct
     5461 attggcaatc tcgcgcggtc atatctttaa tgatggtaat aagcgcaccg cactgtttat
     5521 cacgctatta ttccttaaac gtaacggcat catacttgct gcaaacccag agtttgtaga
     5581 attgacggtt gatgtggcgg cgggaaaagt atcgttagaa gatatcgtta cccgcttgcg
     5641 tcaacgcggt acctgacaag gttcttagcc attctgcttt ttatccgtgc cccatctccc
     5701 attttccctc acccacgccg tcaccgcctt gtcatctttc tgacacctta ctatcttaca
     5761 aatgtaacaa aaaagttatt tttctgtaat tcgagcatgt catgttaccc cgcgagcata
     5821 aaaagcgtga attcgcgcat ccggtacaac aagagagata aacgatgaaa ccgttacatt
     5881 atacagcttc agcactggcg ctcggactgg cgttaatggg gaatgcacag gcagtgacga
     5941 ccattccgtt ctggcattct atggaagggg aactgggtaa agaggtggat tctctggccc
     6001 aacgttttaa cgccgaaaac ccggattaca aaattgtacc gacctataaa ggcaactacg
     6061 aacagaattt aagcgcgggg attgccgcat ttcgtaccgg caacgcgccg gctattttgc
     6121 aggtttatga agttggcacc gccaccatga tggcgtcgaa agccattaaa ccggtatatg
     6181 acgtgtttaa agaggcgggg attcagttcg atgagtcgca gtttgtgccg acggtttcag
     6241 gctactactc cgacagcaaa accggccact tactctccca gccgttcaat agctcgactc
     6301 ctgttctcta ttacaacaaa gacgccttca agaaagccgg tttagacccg gaacagccgc
     6361 cgaaaacctg gcaggatctg gcggactatg ccgcgaaact gaaagcctcc ggtatgaagt
     6421 gcggctacgc cagcggctgg cagggctgga tccaactgga aaactttagc gcctggaacg
     6481 gtctgccgtt tgccagcaaa aacaacggct ttgacggcac agacgcggtg ctggagttca
     6541 acaagccgga gcaggtgaaa cacatcgcta tgctcgaaga gatgaacaag aagggcgatt
     6601 tcagctacgt cgggcgtaag gatgaatcca ctgagaagtt ctataacggt gattgcgcga
     6661 tgaccaccgc ctcttccggt tcgcttgcca acattcgtga gtacgccaaa tttaactacg
     6721 gcgtaggcat gatgccttac gacgccgatg cgaaagatgc accgcaaaac gccattatcg
     6781 gcggggccag cctgtgggtg atgcagggca aagataaaga aacgtatacc ggcgtggcga
     6841 agttcctcga cttcctcgcg aagccagaaa acgctgccga gtggcatcag aaaaccggtt
     6901 atctgccaat caccaaagcg gcgtatgacc tgacccgtga gcagggcttt tatgagaaaa
     6961 acccaggggc ggataccgcg acgcgtcaga tgctgaataa gccgccgtta ccgttcacca
     7021 aagggctgcg tctgggcaac atgccgcaga tccgcgtgat tgtggatgaa gagctggaga
     7081 gcgtgtggac cggtaagaag acaccgcagc aggcgctgga taccgccgtt gagcgtggga
     7141 accagttact gcgccgcttt gagcagtcta cgaagtctta attctcttac cctcacccta
     7201 accctctccc aaagggagag gggaccgatc ggcgtggtat cctggtgccg tgtcggtttg
     7261 caacttcgct cggtgggaaa gtatgcaccg cctttctctc tcgcccctct ggggagaggg
     7321 ccggggtgag gggaacacaa cacgaagagt ttaactatgt catcatcccg tccggtgttc
     7381 cgctcgcgct ggctgcccta tttgctggtc gcgccgcagc tcatcatcac cgttatcttt
     7441 tttatctggc ctgcgggcga agcgttgtgg tactcgctgc aaagcgtcga tccgtttggt
     7501 ttctccagcc agtttgtcgg cctggataac ttcgtcacgc tgtttcatga cagctactat
     7561 ctcgactcct tctggacgac gataaaattc agcacctttg tcaccgtcag cggtttgctg
     7621 gtgtcgctgt tctttgcggc gctggtggag tacatcgtgc gcggcagccg tttctatcaa
     7681 accttaatgc tgctgcctta cgctgtggct cccgccgttg ccgccgtatt gtggatcttc
     7741 ctgtttaacc ccggtcgcgg gctgatcacc cattttctcg ctgagttcgg ctacgactgg
     7801 aaccacgcgc aaaacagcgg tcaggcgatg tttctggtgg tgtttgcctc ggtgtggaag
     7861 caaatcagct acaactttct gttcttctat gccgcgctgc aatccattcc ccgttcgttg
     7921 atcgaagccg cagccatcga cggtgcaggc ccgattcgcc gcttctttaa gattgcgctg
     7981 ccgcttatcg ctccggtgag tttcttcctg ctggtggtga acctggtgta cgccttcttc
     8041 gataccttcc cggtgatcga cgccgccacg tccggcgggc cggttcaggc caccacgacg
     8101 ctgatttata agatttaccg cgaaggcttt accggactgg atctggcttc gtctgccgcg
     8161 cagtcggtgg tgttgatgtt cctcgtcatc gtgctgacgg tggtgcagtt ccgctatgtt
     8221 gaaagcaagg tgcgttacca atgattgaga accgtccgtg gctgacaata ttcagccata
     8281 ccatgctgat cctcgggatc gcggtgatcc tcttcccgct gtacgtggcg tttgtcgcgg
     8341 cgacactgga taaacaagcc gtctacgccg cgccgatgac gctaatcccc ggcacgcatc
     8401 tgctggaaaa catccacaac atctgggtga acggggtagg cacgaatagc gcgccgttct
     8461 ggcggatgtt gcttaacagc tttgtgatgg cgttcagcat tacgctcggc aaaattaccg
     8521 tctcgatgct ctcggccttt gccattgtct ggtttcgttt tccactacgt aacctcttct
     8581 tctggatgat ttttatcacc ctgatgctgc cggttgaagt gcgtatcttc ccgacggtgg
     8641 aagtcatcgc caacctgaaa atgctcgaca gctacgcagg tttaacgctg ccgctgatgg
     8701 cctcggcgac cgccacgttt ctgtttcgcc agttctttat gaccctaccg gatgaactgg
     8761 tggaagccgc gcggatcgac ggcgcgtcac caatgcgttt cttttgcgac atcgtttttc
     8821 cgctgtccaa aaccaatctg gcggcgctgt ttgtgatcac ctttatctac ggctggaacc
     8881 agtatttgtg gccgttgttg attattaccg acgttgatct cggcaccacc gtggcaggga
     8941 ttaaagggat gatcgccaca ggcgaaggca caacggaatg gaactcggtg atggcggcga
     9001 tgttgttaac cctgatccca ccagtagtga ttgttttagt gatgcagcgt gccttcgtgc
     9061 gcggcctggt cgatagtgag aaataagatg gcaggactga aattacaggc agtaaccaaa
     9121 agctgggatg gcaaaaccca ggtcattaaa ccgctgaccc ttgatgtggc ggatggcgaa
     9181 tttatcgtga tggtcgggcc gtctggctgc gggaaatcga cgttattgcg aatggttgcc
     9241 ggactggagc gggtaacgac gggcgatatc tggatcgacc gcaagcgtgt caccgaaatg
     9301 gagccgaaag atcgcgggat tgcgatggtg ttccagaact acgcgcttta tccgcatatg
     9361 agcgtcgaag aaaacatggc gtgggggctg aaaattcgcg gcatgggcaa gcagcaaatt
     9421 gccgagcgcg ttaaagaagc ggcgcgcatt ctggagctgg acggcctgct taagcgccgc
     9481 ccgcgtgagc tatctggcgg tcagcgtcag cgtgtggcga tggggcgtgc gattgtgcgt
     9541 gagccggcag tgttcctgtt tgatgagccg ctctctaacc tcgatgccaa gttgcgtgtg
     9601 cagatgcgtc ttgaactgca acagctgcac cgtcgcctga aaacgacttc actctacgtt
     9661 acccacgatc aggttgaggc gatgacgctc gcccagcgag taatggtgat gaacggcggc
     9721 gttgccgaac agattggcac gccagttgaa gtgtacgaaa agcccgccag cctgtttgtg
     9781 gcgagtttta tcggcagtcc ggcgatgaat ctgctgaaag ggcgtgtgaa taacgaaggt
     9841 acgcatttcg aactggacgg cggtatcgcg ctgccgctaa acggtggcta ccgtcagtat
     9901 gctggacgta aaatgactct cggcattcgc ccggaacata ttacgctacg ttcgcaggct
     9961 gaaggtggcg tgccgctggt gatggacacg ctggagatcc tcggcgcaga taacctggcg
    10021 cacggacgct ggggcgaaca gaagctggtg ttgcgactgg cgcatcagga gcgcccgacg
    10081 gcaggcagca cgctgtggct gcatctgccg gaaaatcagc tacatctttt tgatggtgaa
    10141 acaggacaac gagtatgagt aactggcctt atccccgcat cgtcgcccat cgtggcggcg
    10201 gtaaactggc cccggaaaac accctggcgg caatcgatgt gggggcaaga tacggtcata
    10261 agatgatcga atttgacgcg aagttatcga aagatggcga gatcttcttg cttcatgacg
    10321 acaatctcga acgcaccagc aacggctggg gcgtcgcggg tgaactgaac tggcaggatt
    10381 tactgcgcgt ggatgcgggc agttggtaca gcaaagcgtt taaaggtgag ccgctgccgt
    10441 tgctttcgca ggtggcggaa cgctgtcgcg aacacgggat gatggcgaat atcgaaatca
    10501 aacccaccac tggcaccggg ccattaacgg gcaaaacggt ggcgctggcg gcacgcgaac
    10561 tgtggaccgg tatgacgccg ccgctgctgt catcgtttga gattgatgct ttagaagccg
    10621 cgcaacaggc agcaccggaa ctgccgcgtg gtttattgct tgatgaatgg cgcgacgact
    10681 ggcgagaatt gaccgcgcgg ctgggctgcg tctctattca tctcaatcat aagttgctcg
    10741 ataaagcgcg agtgatgcag ttgaaagacg ccggattacg gattctggtt tataccgtca
    10801 ataaacccca gcgcgcggca gagttgctgc gctggggcgt ggattgcatc tgtaccgatg
    10861 cgattgacgt gattggtccg aactttacgg cccaatagtt ttcaacggaa tgtcaggctg
    10921 cggcgtgctg ggctgattca gcatgtcgcc gtttctcctt tccggcagca tatgctgctg
    10981 attcaacgaa ctatccggat tacggttgct gtttaacatc ccgccattgg tgttgggcag
    11041 catttgctgt cgggcgggat ttcgctcccc cggctgtgac tgcaacaccc gctgagaatt
    11101 attgtttatc tggttttcta aatgctgctg ttgcagctgc gtttgcgttt tcagttgctg
    11161 attcagcatc cctttttgct ggatttgctg cgtttgcatt tgggtttgca tccgctgctg
    11221 actggggatc tgataccccg gctggttagg gttattcaga gtattaatgg gctgtgcaaa
    11281 gccgacaaac ggcaggagtg ccgtaagaat cagaagtcgt ttcatcgcgt atcctcctct
    11341 gaagatattc tttaagttta ctcctttcct gcaatcatca ggattaattc agagttatat
    11401 accaggctta gctggggttg ccccttaatc tctggagaat aacgatgata aaaccgacgt
    11461 ttttacgccg ggtggccatt gctgctctgt tcacaggaag ttgttttagt acggtagccg
    11521 cgccacctgc gccgtctcct gtttcttacg gcgtggagga agatgtcttt catccggtac
    11581 gcgcgaaaca ggggatggtg gcgtctgtag atgctactgc cactcaggtg ggggtggata
    11641 tcctcaagga gggcgggaat gccgttgatg ccgccgtggc tgtgggctac gcgctggcgg
    11701 taacgcatcc gcaggcaggg aatctgggcg gtggtggttt tatgctgatc cgctcgaaaa
    11761 atggcaatac caccgctatc gatttccgcg aaatggctcc cgccaaagcg acccgcgata
    11821 tgttccttga tgatcagggt aacccggaca gcaaaaaatc actcacttcg catctggctt
    11881 ctggtacgcc cggtacggta gcaggtttct cgctggcgtt ggataaatac ggcaccatgc
    11941 cgttgaacaa agtggtgcaa ccggcgttta aactggcacg cgatggtttt atcgttaacg
    12001 acgcgttggc tgacgatctc aaaacctacg gtagcgaagt gttgccgaat cacgaaaaca
    12061 gcaaggctat cttctggaaa gagggcgcgc cgctgaaaaa gggcgacaag ctggtgcagg
    12121 cgaacctggc aaagagcctg gagatgattg ccgaaaacgg cccggacgaa ttctacaaag
    12181 gcacgatagc ggaacagatt gcccaggaga tgcagaaaaa cggtggcttg atcactaaag
    12241 aagatttagc ggcgtataaa gcggtcgaac gtacaccgat tagcggcgaa tatcgcgggt
    12301 atcaggttta ctcgatgcca ccgccatctt ccggtgggat tcatatcgtg cagatcctca
    12361 atatcctgga aaacttcgat atgcagaaat acggctttgg cagcgccgat gcgatgcaaa
    12421 tcatggcaga agcggagaaa tacgcctacg ccgaccgctc ggaatatctt ggcgatccgg
    12481 attttgtcaa agtcccgtgg caggcgctga ccaataaagc ctatgccaaa tctattgccg
    12541 atcaaatcga tatcaataaa gcgaagccat ccagcgaaat tcgccctggc aagcttgcgc
    12601 cttatgagag taatcaaact acccattact cagtggtgga taaagatggt aacgcggtgg
    12661 cggtgaccta tacgctgaac accaccttcg gtacgggcat tgtcgcgggc gagagcggta
    12721 ttctgcttaa taaccaaatg gatgatttct ccgccaaacc gggcgtgccg aacgtttacg
    12781 ggctggtggg tggtgacgct aacgcggtcg ggccgaataa acgtccgctg tcgtcgatgt
    12841 caccaaccat tgtggtgaaa gacggtaaaa cctggctggt caccggaagc ccaggcggta
    12901 gccggatcat cactaccgtg ctgcaaatgg tggtgaatag catcgattat ggtatgaatg
    12961 tcgccgaagc gaccaatgca ccgcgtttcc accatcagtg gttgccggat gagctgcgtg
    13021 tcgagaaagg atttagcccg gatacgctca agctgctgga agcaaaaggt cagaaagtgg
    13081 cgctgaaaga ggcgatgggc agtacacaaa gcattatggt tgggcctgac ggtgagttgt
    13141 acggcgcatc cgacccgcgc tcagtagatg atttaacggc ggggtattaa ggttagcggc
    13201 cctcttcgtg ggaagagggc cgtattgtca gggcaagccg aaggtagcct tttttatttc
    13261 ataatcctgt agatattctt ccagcggctt tgccgttcca agggaatgaa tttcaccact
    13321 gtctttatca ataataaaag gtgcgttacc ggctaagcgc gcggcctcat caccagattc
    13381 aagaaattct tgtgattcga aacagaaata ccagccctgg ataaaacgtc catgcagagt
    13441 gatgacgaca gggagatctg catcatcaag gtaatggttc gctttcgcga atgcg
//
LOCUS       DAZFLD010000132        13226 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.133, whole genome shotgun sequence.
ACCESSION   DAZFLD010000132 DAZFLD010000000
VERSION     DAZFLD010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13226)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13226)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13226
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.133"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..211)
                     /locus_tag="ACHKBL_003281"
     CDS             complement(<1..211)
                     /locus_tag="ACHKBL_003281"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5820113.1"
                     /translation="MRFLLANTFCKIVKQFSFIDRIRIYSLHPCLNKHLSIQSNRDWL
                     SMSLFSDYSSSSEMHNNLTIDYYLAL"
     gene            495..2126
                     /gene="oppA"
                     /locus_tag="ACHKBL_003282"
     CDS             495..2126
                     /gene="oppA"
                     /locus_tag="ACHKBL_003282"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HFO5820114.1"
                     /translation="MTNITKRSLVAAGVLAALMAGNVALAADVPAGVTLAEKQTLVRN
                     NGSEVQSLDPHKIEGVPESNISRDLFEGLLVSDLDGHPAPGVAESWDNKDAKVWTFHL
                     RKDAKWSDGTPVTAQDFVYSWQRSVDPNTASPYASYLQYGHIAGIDEILEGKKPITDL
                     GVKAIDDHTLEVTLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIITNGAYT
                     LKDWVVNERIVLERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPI
                     ELFQKLKKEIPDEVHVDPYLCTYYYEINNQKPPFNDVRVRTALKLGMDRDIIVNKVKA
                     QGDMPAYGYTPPYTDGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPLTINLLYN
                     TSDLHKKLAIAASSLWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPT
                     SFLNTMLSNSSMNTAHYKSPAFDSIMAETLKATDEAQRTALYTKAEQQLDKDSAIVPV
                     YYYVNARLVKPWVGGYTGKDPLDNTYTRNMYIVKH"
     gene            2212..3132
                     /gene="oppB"
                     /locus_tag="ACHKBL_003283"
     CDS             2212..3132
                     /gene="oppB"
                     /locus_tag="ACHKBL_003283"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HFO5820115.1"
                     /translation="MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPE
                     VMANIEAKYHLNDPIMTQYFSYLKQLAHGDFGPSFKYKDYSVNDLVASSFPVSAKLGA
                     AAFFLAVILGVSAGVIAALKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHW
                     LPGGGWNGGALKFMILPMVALSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPMRR
                     IILRHALKPALLPVLSYMGPAFVGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGALTILFNAIVDVLYAVIDPKIRY"
     gene            3147..4055
                     /gene="oppC"
                     /locus_tag="ACHKBL_003284"
     CDS             3147..4055
                     /gene="oppC"
                     /locus_tag="ACHKBL_003284"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HFO5820116.1"
                     /translation="MMLSKKNSETLENFSEKLEVEGRSLWQDARRRFMHNRAAVASLI
                     VLVLIALFVILAPMLSQFAYDDTDWAMMSSAPDMESGHYFGTDSSGRDLLVRVAIGGR
                     ISLMVGVAAALVAVVVGTLYGSLSGYLGGKVDSVMMRLLEILNSFPFMFFVILLVTFF
                     GQNILLIFVAIGMVSWLDMARIVRGQTLSLKRKEFIEAAQVGGVSTSGIVIRHIVPNV
                     LGVVVVYASLLVPSMILFESFLSFLGLGTQEPLSSWGALLSDGANSMEVSPWLLLFPA
                     GFLVVTLFCFNFIGDGLRDALDPKDR"
     gene            4067..5080
                     /gene="oppD"
                     /locus_tag="ACHKBL_003285"
     CDS             4067..5080
                     /gene="oppD"
                     /locus_tag="ACHKBL_003285"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP-binding protein OppD"
                     /protein_id="HFO5820117.1"
                     /translation="MSVIETATVPLAQQQADALLNVKDLRVTFSTPDGDVTAVNDLNF
                     SLRAGETLGIVGESGSGKSQTAFALMGLLAANGRIGGSATFNGREILNLPERELNRLR
                     AEQISMIFQDPMTSLNPYMRVGEQLMEVLMLHKNMSKAEAFEESVRMLDAVKMPEARK
                     RMKMYPHEFSGGMRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKREF
                     NTAIIMITHDLGVVAGICDKVLVMYAGRTMEYGNARDVFYQPVHPYSIGLLNAVPRLD
                     AEGETMLTIPGNPPNLLRLPKGCPFQPRCPHAMEICSSAPPLEEFTPGRLRACFKPVE
                     ELL"
     gene            5077..6081
                     /gene="oppF"
                     /locus_tag="ACHKBL_003286"
     CDS             5077..6081
                     /gene="oppF"
                     /locus_tag="ACHKBL_003286"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP-binding protein OppF"
                     /protein_id="HFO5820118.1"
                     /translation="MNAVTEGRKVLLEIADLKVHFEIKDGKQWFWQPPKTLKAVDGVT
                     LRLYEGETLGVVGESGCGKSTFARAIIGLVKATDGHVAWLGKELLGMKPDEWRAVRSD
                     IQMIFQDPLASLNPRMTIGEIIAEPLRTYHPKMSRQEVRERVKAMMLKVGLLPNLINR
                     YPHEFSGGQCQRIGIARALILEPKLIICDEPVSALDVSIQAQVVNLLQQLQREMGLSL
                     IFIAHDLAVVKHISDRVLVMYLGHAVELGTYDEVYHNPLHPYTRALMSAVPIPDPDLE
                     KNKTIQLLEGELPSPINPPSGCVFRTRCPIAGPECAKTRPVLEGSFRHAVSCLKVDPL
                     "
     gene            complement(6134..6463)
                     /gene="yciU"
                     /locus_tag="ACHKBL_003287"
     CDS             complement(6134..6463)
                     /gene="yciU"
                     /locus_tag="ACHKBL_003287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HI1450 family dsDNA-mimic protein"
                     /protein_id="HFO5820119.1"
                     /translation="MDMDLNNRLTEDETLEQAYDIFLELAADNLDPADVLLFNLQFEE
                     RGGAELFDPAEDWQEHVDFDLNPDFFAEVVIGLADSEDGEINDVFARILLCREKDHKL
                     CHIIWRE"
     gene            complement(6498..7958)
                     /gene="cls"
                     /locus_tag="ACHKBL_003288"
     CDS             complement(6498..7958)
                     /gene="cls"
                     /locus_tag="ACHKBL_003288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFO5820120.1"
                     /translation="MTTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLII
                     YILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEENSSVAAP
                     LFKLCERRQGIAGVKGNQLQLMTESDDVMQALIRDIQLARHNIEMVFYIWQPGGMADQ
                     VAESLMAAARRGIHCRLMLDSAGSVAFFRSPWPELMRNAGIEVVEALKVNLMRVFLRR
                     MDLRQHRKMIMIDNYIAYTGSMNMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIY
                     SCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPEDLIHQALLTAAYSAR
                     EYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAG
                     VKIYQFEGGLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQ
                     DDYISRSRLLDARLWLKRPLWQRVAERLFYFFSPLL"
     gene            8101..8274
                     /gene="yciY"
                     /locus_tag="ACHKBL_003289"
     CDS             8101..8274
                     /gene="yciY"
                     /locus_tag="ACHKBL_003289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciY family protein"
                     /protein_id="HFO5820121.1"
                     /translation="MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILN
                     KQRNSLLFAIYNI"
     gene            complement(8329..9582)
                     /gene="kch"
                     /locus_tag="ACHKBL_003290"
     CDS             complement(8329..9582)
                     /gene="kch"
                     /locus_tag="ACHKBL_003290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated potassium channel protein"
                     /protein_id="HFO5820122.1"
                     /translation="MSHWATFKQTATNLWVTLRHDILALAVFLNGLLIFKTIYGMSVN
                     LLDIFHIKAFSELDLSLLANAPLFMLGVFLVLNSIGLLFRAKLAWAISIILLLIALIY
                     TLHFYPWLKFSIGFCIFTLVFLLILRKDFSHSSAAAGTIFAFISFTTLLFYSTYGALY
                     LSEGFNPRIESLMTAFYFSIETMSTVGYGDIVPVSESARLFTISVIISGITVFATSMT
                     SIFGPLIRGGFNKLVKGNNHTMHRKDHFIVCGHSILAINTILQLNQRGQNVTVISNLP
                     EDDIKQLEQRLGDNADVIPGDSNDSSVLKKAGIDRCRAILALSDNDADNAFVVLSAKD
                     MSSDVKTVLAVSDSKNLNKIKMVHPDIILSPQLFGSEILARVLNGEEINNDMLVSMLL
                     NSGHGIFSDNDEQETKADSKESAQK"
     gene            complement(9883..10179)
                     /gene="yciI"
                     /locus_tag="ACHKBL_003291"
     CDS             complement(9883..10179)
                     /gene="yciI"
                     /locus_tag="ACHKBL_003291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HFO5820123.1"
                     /translation="MLYVIYAQDKADSLEKRLSVRPAHLARLQLLHDEGRLLTAGPMP
                     AVDSNDPGAAGFTGSTVIAEFESLEAAQAWADADPYVAAGVYEHVSVKPFKKVF"
     gene            10403..11122
                     /gene="tonB"
                     /locus_tag="ACHKBL_003292"
     CDS             10403..11122
                     /gene="tonB"
                     /locus_tag="ACHKBL_003292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein TonB"
                     /protein_id="HFO5820124.1"
                     /translation="MTLDLPRRFPWPTLLSVCIHGAVVAGLLYTSVHQVIELPAPAQP
                     ISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPKPKP
                     VKKVQEQPKRDVKPVESRPASPFENTAPARPTSSTATAATSKPVTSVASGPRALSRNQ
                     PQYPARAQALRIEGQVKVKFDVTPDGRVDNVQILSAKPANMFEREVKNAMRRWRYEPG
                     KPGSGIVVNILFKINGTTEIQ"
     gene            complement(11162..11560)
                     /gene="yciA"
                     /locus_tag="ACHKBL_003293"
     CDS             complement(11162..11560)
                     /gene="yciA"
                     /locus_tag="ACHKBL_003293"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HFO5820125.1"
                     /translation="MSTTHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAI
                     LAKEIAHGRVVTVRVEGMTFLRPVAVGDVVCCYARCVQKGTTSVSINIEVWVKKVASE
                     PIGQRYKATEALFKYVAVDPEGKPRALPVE"
     gene            complement(11665..12204)
                     /gene="yciB"
                     /locus_tag="ACHKBL_003294"
     CDS             complement(11665..12204)
                     /gene="yciB"
                     /locus_tag="ACHKBL_003294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HFO5820126.1"
                     /translation="MKQFLDFLPLVVFFAFYKIYDIYAATAALIVATAIVLIYSWVRF
                     RKVEKMALITFVLVVVFGGLTLFFHNDEFIKWKVTVIYALFAGALLVSQWVMKKPLIQ
                     RMLGKELTLPQPVWSKLNLAWAVFFILCGLANIYIAFWLPQNIWVNFKVFGLTALTLI
                     FTLLSGIYIYRHMPQEDKS"
     gene            complement(12234..12977)
                     /gene="yciC"
                     /locus_tag="ACHKBL_003295"
     CDS             complement(12234..12977)
                     /gene="yciC"
                     /locus_tag="ACHKBL_003295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciC family protein"
                     /protein_id="HFO5820127.1"
                     /translation="MSITAQSVYRDTGNFFRNQFMTILLVSLLCAFITVVLGHVFSPS
                     DAQLAQLNDGVPVSGSSGLFDLVQNMSPEQQQILLQASAASTFSGLIGNAILAGGVIL
                     IIQLVSAGQRVSALRAIGASAPILPKLFILIFLTTLLVQIGIMLVVVPGIIMAILLAL
                     APVMLVQDKMGVFASMRSSMRLTWANMRLVAPAVLSWLLAKTLLLLFASSFAALTPEI
                     GAVLANTLSNLISAVLLIYLFRLYMLIRQ"
     gene            complement(13005..13094)
                     /locus_tag="ACHKBL_003296"
                     /pseudo
     CDS             complement(13005..13094)
                     /locus_tag="ACHKBL_003296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
BASE COUNT         3563 a         3137 c         3249 g         3277 t
ORIGIN      
        1 aaagtgctaa ataataatca attgttaaat tattgtgcat ttcactactg gaactgtaat
       61 cagaaaagag agacatgctt aaccaatctc tatttgattg aattgaaaga tgtttgttaa
      121 gacatggatg caagctatag attctgatgc ggtcaataaa agagaattgc ttaacaattt
      181 tgcaaaatgt attggcgagt aagaagcgca tttggtactt tccgggcaac cgccaggcga
      241 ttctttattg gtaatgagaa taattaacaa ttaaagagcg tcgcgaaaga ataatgtgtc
      301 tcgacagggg agacacagta cgaatcgaca taaggtgatc gtctgaatca ccagaataaa
      361 taaagtcggt gatagcaatc cgtaacgata aagtaacctg acagcagaaa gtctccgagc
      421 ctgtgcaggg tcccaatccg ggattacaca tgctggttaa taccagtaat tataatgagg
      481 gagtccaaaa aacaatgacc aacatcacca agagaagttt agtagcagct ggcgttctgg
      541 ctgcactaat ggcagggaat gtcgcgctgg cagctgatgt acccgcaggc gtcacactgg
      601 cggaaaaaca aacgctggta cgtaacaatg gttcagaagt tcagtcatta gatccgcata
      661 aaattgaagg tgttccggag tctaatatca gccgagacct gtttgaaggc ttactggtca
      721 gcgatcttga cggtcatccg gcacctggcg tcgctgaatc ctgggataat aaagacgcga
      781 aagtctggac cttccatttg cgtaaagatg cgaaatggtc tgatggcacg ccagtcacag
      841 cacaagactt tgtgtatagc tggcaacgtt ctgttgatcc aaacaccgct tctccgtatg
      901 ccagttatct gcaatatggg catatcgccg gtattgatga aattcttgaa gggaaaaaac
      961 cgattaccga tctcggcgtg aaagcaattg atgatcacac attagaagtc accttaagtg
     1021 aacctgttcc gtacttctat aaattacttg ttcacccatc aacttcaccg gtgccaaaag
     1081 ccgctatcga gaaattcggc gaaaaatgga cccagcctgg taatatcatc accaacggtg
     1141 cctatacctt aaaagattgg gtcgtaaacg aacgaatcgt tcttgaacgc agcccaacct
     1201 actggaacaa cgcgaaaacc gttattaacc aggtaaccta tttgcctatt gcttctgaag
     1261 ttaccgatgt caaccgctac cgtagtggtg aaatcgacat gacgtataac aacatgccga
     1321 tcgaattgtt ccagaagctg aaaaaagaga tcccggacga agttcacgtt gatccatacc
     1381 tgtgcactta ttattacgaa attaacaacc agaaaccgcc attcaacgat gtacgtgtgc
     1441 gtaccgcact gaaactgggt atggaccgcg atatcattgt taataaagtg aaagcgcagg
     1501 gcgacatgcc cgcctatggt tacactccac cgtatactga tggcgcaaaa ttgactcagc
     1561 cggaatggtt tggctggagc caggaaaaac gtaacgaaga agcgaaaaaa ctgctggctg
     1621 aagcgggtta taccgcagac aaaccgttga ccatcaacct gttgtataac acctccgatc
     1681 tgcataaaaa gctggcgatt gctgcctctt cattgtggaa gaaaaacatt ggtgtaaacg
     1741 tcaaactggt taaccaggag tggaaaacgt tcctcgacac ccgtcaccag ggcacttttg
     1801 atgtggcccg tgcaggctgg tgtgcagact acaacgaacc aacttccttc ctgaacacca
     1861 tgctgtcgaa cagctcaatg aataccgcgc attataagag cccggccttt gacagcatta
     1921 tggcggaaac gctgaaagcg actgacgagg cgcagcgcac agctctgtac actaaagcag
     1981 aacaacagct ggataaggat tcggccattg ttcctgttta ttactacgtg aatgcgcgtc
     2041 tggtgaaacc gtgggttggt ggctataccg gcaaagatcc gctggataat acctacaccc
     2101 ggaatatgta cattgtgaag cactaatggc aatacgtggg gcaggcgtgt actgctccac
     2161 ggtgtctgat ttttatcgca ttacagaagg cacaggccag aaggtagggc aatgttaaaa
     2221 tttattctac gtcgctgtct ggaagcgatt ccgacgctat ttattcttat tactatttcg
     2281 ttcttcatga tgcgcctcgc gccgggaagc ccttttaccg gcgaacgtac tttaccgcca
     2341 gaagtgatgg ccaatatcga agcgaaatat catcttaatg atccgatcat gacacagtat
     2401 ttcagctacc tgaaacaact ggcgcatggc gatttcggtc catcgtttaa atataaagat
     2461 tattcggtca acgacctggt tgcatccagt tttcccgttt ctgccaaact gggagccgca
     2521 gcatttttcc ttgcggtaat actgggtgtt agtgctggcg ttattgccgc attaaaacaa
     2581 aacaccaaat gggactatac cgtgatgggg ctggcaatga ccggggttgt tatccccagt
     2641 tttgtggttg cgccattatt agtcatgata tttgcgatca ttttgcattg gctgccgggc
     2701 ggtggctgga atggtggtgc gcttaaattc atgatactgc caatggtggc gttgtcactc
     2761 gcctatatcg ccagtattgc gcgtattacc cgtggctcta tgattgaagt attacactcc
     2821 aactttattc gtactgcccg ggcgaaaggg ttacctatgc ggcggatcat tttacgccac
     2881 gcattaaaac ctgctctgtt acccgtgctc tcctatatgg gccccgcatt tgtcggcatt
     2941 attaccggtt ctatggtcat cgaaaccatt tatggtttgc cggggattgg gcaactgttc
     3001 gttaatggcg cattgaaccg tgactattcc ttagtgttaa gcctgactat cctggttggt
     3061 gcgttaacca ttttgtttaa tgccattgtc gatgtgctat atgcggttat cgacccgaaa
     3121 atccgttact gatactggag ctcgcgatga tgttaagtaa gaaaaacagc gagacgctgg
     3181 aaaatttcag tgaaaagctg gaggtcgaag ggcgcagctt gtggcaggac gcacgtcgac
     3241 gttttatgca taaccgtgcg gcggttgcca gtctgatagt gctggtgcta atcgcgttat
     3301 ttgtaatcct ggcaccgatg ctttcgcagt ttgcctatga cgatactgac tgggcgatga
     3361 tgtccagcgc cccggatatg gagtccggtc actactttgg tactgactca tccggtcgcg
     3421 acctgcttgt gcgtgttgcg attggcgggc gtatctcact catggtcggt gttgctgcgg
     3481 cactggtggc agtggtcgtg gggacacttt atggttcgct ttccggttat ctgggcggta
     3541 aagtggattc ggtaatgatg cgtctgctgg aaatcctcaa ctccttccca ttcatgttct
     3601 tcgtcatttt gctggtgacc tttttcggtc aaaacatcct gctgattttc gtggcgattg
     3661 gcatggtttc ctggctggat atggctcgta ttgtgcgtgg gcaaaccctg agtctgaagc
     3721 gcaaagagtt tattgaggcg gcacaagttg gcggtgtatc gacgtcgggc attgttattc
     3781 gccacattgt gccgaatgta ctcggtgtgg tggtggtcta cgcatcactt ctggtgccca
     3841 gcatgatcct ctttgaatct ttccttagct tcctggggtt ggggacgcaa gagccgttaa
     3901 gcagctgggg cgcattgctg agtgatggcg cgaactcgat ggaagtctct ccatggttac
     3961 tgttgttccc agcgggattc ctcgtggtga cgctattttg tttcaacttt ataggcgatg
     4021 gcttgcgtga tgccctcgac ccgaaagatc gttaaggagt gcagccatga gcgtaattga
     4081 aactgcaact gtgccgctcg cacaacaaca ggctgacgca ctgctgaacg tgaaagattt
     4141 gcgtgtcacc tttagtaccc cggacggcga cgtcacggcg gtcaatgatt tgaatttttc
     4201 cctacgtgcc ggagaaacgc tgggcattgt aggtgagtct ggttcgggta aatcgcaaac
     4261 tgcatttgcg ttgatgggcc tgctggctgc caacggacgt attggcggat cggcaacctt
     4321 caatgggcgt gaaatcctca atttgccaga gcgtgaactc aatagactgc gcgctgaaca
     4381 aatctcaatg atttttcagg acccaatgac ttcgttgaat ccctatatgc gcgtcggtga
     4441 gcagttgatg gaagtgctga tgctgcataa gaacatgagc aaagctgagg cgtttgaaga
     4501 gtcggtgcgg atgctcgatg cggtaaaaat gccggaagca cgtaaacgca tgaaaatgta
     4561 cccgcatgaa ttttctggtg gcatgcgtca gcgagtcatg attgcgatgg cattgctatg
     4621 tcgacctaag ctgctgattg cggatgaacc cactactgcg ctggacgtca ccgtacaggc
     4681 gcagatcatg acgctattaa atgaactgaa gcgggaattt aataccgcca tcattatgat
     4741 cacccacgat cttggcgtgg tggcggggat ctgcgacaaa gtgctggtaa tgtacgccgg
     4801 gcgcacgatg gaatatggca acgcgcgcga tgtcttttat caacccgttc atccttattc
     4861 tatcggtttg ctcaacgcgg tgccgcgtct cgatgcggaa ggtgaaacaa tgttgaccat
     4921 ccctggtaat ccgccaaacc tgctgcgatt accgaaaggt tgcccgttcc agccacgttg
     4981 tccgcatgcg atggaaattt gtagtagcgc accgccgctg gaagagttta cgcctggccg
     5041 tctgcgtgct tgctttaaac cggtggagga actgttatga atgctgtaac cgaaggaaga
     5101 aaagtcctcc tcgaaatcgc cgatctgaaa gtgcactttg aaatcaaaga tggcaaacag
     5161 tggttctggc aaccgccgaa aacgctcaaa gccgtcgatg gtgtcactct tcgcctgtat
     5221 gaaggggaaa cattaggtgt ggtaggggaa tcgggatgcg gtaagtccac ctttgctcgc
     5281 gccatcattg gtttggtcaa ggcgaccgac ggtcatgttg cctggttagg taaagagttg
     5341 ctgggcatga agcccgatga atggcgtgcc gttcgcagtg atattcagat gattttccag
     5401 gatccattgg catcgcttaa cccgcgtatg accatcggcg agatcatcgc tgaaccactg
     5461 cgtacttatc atccgaaaat gtcacgccag gaagttcgcg agcgcgtgaa ggcgatgatg
     5521 ctgaaagtcg ggttattgcc taacctgatt aaccgctatc cgcatgagtt ctctggtggg
     5581 cagtgccagc gtatcgggat tgctcgtgct cttattcttg aaccgaagct gattatctgt
     5641 gatgagccgg tgtcggcgct ggacgtgtca attcaggcgc aggtggtcaa cctgctccag
     5701 cagctgcaac gcgagatggg attgtcatta atttttatcg ctcatgacct ggccgtggta
     5761 aaacacattt ccgatcgtgt gctggtgatg tatctcggcc atgcggtaga actggggacc
     5821 tatgatgagg tctatcacaa tccgctacat ccttacacca gggcattgat gtcggcagtc
     5881 cccatacctg atccggatct ggagaagaac aaaaccatcc agttactgga aggggaatta
     5941 ccgtcgccga tcaacccgcc ttccggttgt gttttccgta cccgttgccc gattgccggt
     6001 ccagagtgtg ccaaaacacg tcctgtgctg gaggggagtt tcagacacgc cgtttcttgc
     6061 ctgaaagtcg atccgcttta aaagcaataa gggctgacag ttgtcagccc ttattcacgc
     6121 caaaagcgat tatttattcc cgccagatga tatggcacag tttgtgatct ttttcgcggc
     6181 ataacagaat gcgcgcgaaa acatcgttga tttcaccatc ttcactgtcc gccagaccaa
     6241 tcaccacttc ggcaaaaaag tccgggttca aatcgaaatc aacatgttcc tgccagtctt
     6301 ctgccggatc gaataactcc gcgccgccgc gctcttcaaa ctgaagattg aacagcagaa
     6361 catcagctgg atcgagattg tcggcagcca gttcgagaaa aatatcgtag gcctgctcaa
     6421 gcgtttcatc ttcagtcagg cgattgttta gatccatatc catagtcact acctgtttaa
     6481 cctctgttgg cgacgtttta cagcaacggg ctgaagaagt aaaacagtcg ctcggcgaca
     6541 cgttgccata atggacgttt tagccataaa cgggcatcga gcagacgtga acgcgaaata
     6601 taatcgtcct gaacggcggc aaggtctgca ccaaaacctt tatcgtcgat tgccagggta
     6661 atctcgaaat ttagccacag actccgcata tcaaggttaa cggtgccaac cagacttagt
     6721 tcaccatcga ccagcacgct cttggtatgc agtaacccgc cttcaaactg ataaatttta
     6781 accccagcag ccagcagttc cgtaaagaat gcgcgactgg cccagccgac cagcatcgag
     6841 tcattttttc gcggaaggat aatactgaca tccaccccgc gctgcgccgc cgtgcaaatc
     6901 gcatgaagta aatcatcgct gggcacaaag tagggcgttg tcatgatcaa atattcacgc
     6961 gccgaataag cagcagtcaa taatgcctgg tgaatgagat cttccggaaa gccggggcca
     7021 gaagcaattg tgtgaatggt gtgaccgctg gcctgctcaa acggcataat attgacatct
     7081 ggtggtggcg gcagaatacg ttttccggtt tcaatctccc agtcgcagga ataaataatc
     7141 cccatcgcgg tagcgatggg accttccata cgcgccatca gatcaatcca ttgccctacg
     7201 cccgcatctt gtttgaagta gcgagggtcg accatattca tgctgccggt gtacgcgatg
     7261 taattatcga tcatgatcat cttgcgatgt tggcgcaggt ccatacggcg taaaaacaca
     7321 cgcatcagat tgacctttaa ggcttcgaca acttcaatac cggcattacg cattagctcg
     7381 ggccacgggc tgcggaaaaa agccacactc ccggcggagt cgagcatcaa tcggcaatga
     7441 atgccgcgtc gcgcagccgc cattaatgat tcagccacct ggtccgccat gccgccgggc
     7501 tgccagatat aaaacaccat ctcaatatta tggcgagcga gctggatgtc gcggattaac
     7561 gcctgcatca catcatctga ctcggtcatc agttgcagct gattcccttt gaccccagcg
     7621 atcccctgac gacgctcgca aagcttgaat aatggcgcag cgacactgct attttcttcg
     7681 gcgaagatat gcttacaggc tttaaggtcg ttaagccatt ttgcggtgga aggccacatc
     7741 gctctggcgc gctcagcgcg gcgtttgcct aaatggagct cgccaacggc aagataggca
     7801 ataattccga ctaacggcag aatgtaaata atcaacagcc aggccatcgc ggagggaact
     7861 gcgcgtcgtt tcattagaat gcgtaaagtt acgcctgcaa tgagcaacca gtatcccaga
     7921 atggccaacc aactcaccaa cgtataaacg gttgtcatag attagaaatc cttttgaaag
     7981 cgcattgtta tgagtttacg catatggatt catctggcaa ataaaaacgc gggaaaagca
     8041 ctggtcagcg tcgggggcgg gggtataatg accattctgt tattgcatag agtagttaac
     8101 atgaagcgga gtagaacgga agtggggcgc tggcgcatgc agcgtcaggc tagccgacgt
     8161 aaatcgcgtt ggcttgaggg gcagtcgcgc cgaaatatgc gtatccacag catcaggaag
     8221 tgcattctaa acaaacagcg taactcgtta ttgtttgcga tctacaatat ctaaatgtac
     8281 tgggcaccat tgcggtgccc aataagttta ctcttccctc tttaaactct atttttgcgc
     8341 cgattcttta ctgtcagctt tcgtttcttg ttcatcgtta tcgctgaaaa tgccatgacc
     8401 ggagttcaac aacattgaaa cgagcatatc attattaatc tcttcaccat ttaaaactcg
     8461 cgccagaatt tcgctgccaa acagttgcgg cgagagaatg atatccggat gtaccatctt
     8521 aatcttattc aggtttttgc tatcactgac ggcgagaact gttttgacat cactgctcat
     8581 atcttttgcc gagagtacaa caaacgcgtt atctgcatcg ttatcactca gcgccagaat
     8641 ggcccggcat cgatcaattc cagctttctt taatactgaa ctgtcattac tgtcaccggg
     8701 gataacatca gcgttatcgc ctaagcgttg ctcaagttgc ttgatatcat cttcaggcaa
     8761 gttgctgata accgttacgt tttgtccgcg ttgattcagt tgcaggatcg tattgatggc
     8821 gagaatcgaa tgtccgcaaa caataaaatg atctttacga tgcattgtat gattgtttcc
     8881 ttttacaagt ttgttgaatc ccccgcggat aagcggacca aaaattgagg tcatagatgt
     8941 ggcaaaaacg gtaatgccgg aaataatgac cgaaatagtg aacaatcgtg ctgattcaga
     9001 aacagggaca atatcgccat agccgacggt tgacatggtt tctatcgaaa aatagaacgc
     9061 ggtcatcaaa ctttctattc gcggattaaa accttcgctt aaataaagcg caccgtaggt
     9121 ggagtaaaac agtaacgtcg tgaaactaat aaatgcgaaa attgtcccgg ctgcggcgct
     9181 actgtgggag aagtctttgc gcagtatcag caaaaacacc agcgtaaaaa tgcaaaatcc
     9241 aatactaaat ttcagccagg gataaaaatg cagggtgtaa attagcgcta tcaacaacaa
     9301 aatgatactg attgcccatg cgagctttgc ccggaacagt aagccaatgg agttcaggac
     9361 aagaaagacg ccgagcataa agagtggggc gtttgccagc aaggagagat ccaactctga
     9421 aaatgctttg atatggaaaa tatctagcaa attgaccgac ataccataga ttgttttaaa
     9481 aataagcaat ccatttaaaa agacggccag cgcgagaatg tcgtgccgta gggtcaccca
     9541 taaattagtt gctgtttgtt tgaatgtagc ccagtgactc actgatacct ctgtaaccac
     9601 aattttgttt aattatctta gcgtgcacat tttctgaaca tacatgcagc gcgtgaatgt
     9661 gttaaacaat agtagatgac tttatccgca gtaatcaaag gttttgagtg agcaactctg
     9721 cgtcaggaaa gggcattttt cctgaatatg ctcacatcat ataaagaaat acagataaag
     9781 ttattatctg cttgtggtgg taaatgcaat gactgaccta taaggaaagg ccaaacaaga
     9841 acacggttgc taaaaccgtg cccttaaata ttgaatttct attcagaaca ctttcttaaa
     9901 tggtttcact gaaacgtgtt catagactcc tgccgctacg tacgggtcag catcggccca
     9961 ggcctgagct gcttccagcg attcaaattc agcaataacg gttgagccag taaatcccgc
    10021 agcccctgga tcgttactgt ctactgctgg cattggaccg gcggtcaaca atcgaccttc
    10081 atcatgcagt aactgtaaac gtgctaaatg tgccggacga acggaaaggc gtttttcgag
    10141 gctatcagct ttgtcttgag cgtaaataac atacaacacg ggcacaactc cttgttcggg
    10201 aaagttgtaa agtacgttat ttgaaagggc gaagatctgc aacggaaaga tgatgtcttt
    10261 gttaagacca tttaaaaagt aaggtcaatt acgccaaaaa tgacattttc actgatcctg
    10321 atcgtcttgc cttattgaat atgattgcta tttgcattta aaatcgaggc ctggtttttc
    10381 tactgaaatg attatgactt caatgaccct tgatttacct cgccgcttcc cctggccgac
    10441 gttactttcg gtctgcattc atggtgctgt tgtggcgggt ctgctctata cctcggtaca
    10501 tcaggttatt gaactacctg cgcctgcgca gccaatttct gtcacgatgg ttgcgcctgc
    10561 ggatctcgaa ccgccacaag ccgttcagcc gccaccggag ccggtggtag agccagaacc
    10621 ggaacctgag ccgatccccg aaccgccaaa agaagcaccg gtggtcattg aaaagccgaa
    10681 gccgaaacct aagccaaaac cgaagccggt gaaaaaggta caggagcagc caaaacgcga
    10741 tgtcaaaccc gtagagtcgc gtccggcatc accgtttgaa aatactgcac ccgcgcgccc
    10801 tacatcaagt acggcaacgg ctgcaaccag caagccggtt accagtgtgg cttcaggacc
    10861 acgcgcatta agccgcaatc agccgcagta tccggcacga gcacaggcat tgcgcattga
    10921 agggcaggtt aaagttaaat ttgatgtcac gccggatggt cgcgtggata acgtacaaat
    10981 cctctcagcc aagcctgcga acatgtttga gcgtgaggtg aaaaatgcga tgcgcagatg
    11041 gcgttatgag ccgggtaagc caggcagtgg gattgtggtg aacatcctgt ttaaaattaa
    11101 cggcaccacc gaaattcagt aagcagaaag tcaaaagcct ccgaccggag gcttttgatt
    11161 attactcaac aggtaaggcg cgaggttttc cttcaggatc aaccgcgaca tacttaaata
    11221 atgcttctgt cgctttatag cgttgcccga tgggttcaga cgcgactttt ttcacccaca
    11281 cttcaatatt aatgctaacc gatgtcgtcc ctttctggac acagcgtgca tagcagcaca
    11341 ccacatcgcc gaccgcaacc ggtcgtaaga aagtcattcc ttcaacccgc acggtcacta
    11401 cgcgaccgtg ggcaatttct ttcgccagaa tagcgccgcc aatatccatt tgtgacatta
    11461 accaaccacc aaagatgtca ccattggcat tggtatcggc gggcatggct aaagtacgta
    11521 aaacaagatc gccctgaggg acgttatgtg ttgtagacat ggtaaaaccg acttaaaagg
    11581 aaaatcacag gcgcgatgct actatgattt tcctgaggag aacagaggag atacagcgcc
    11641 agccccgaag gactggcagt ctggttagga tttatcttcc tgcggcatgt ggcggtagat
    11701 atagataccg cttaacaatg tgaagattaa ggtaagggcg gtcaggccaa agactttaaa
    11761 gttgacccaa atattttgcg gcagccagaa tgcgatgtag atgtttgcca gaccgcaaag
    11821 gataaagaaa acagcccagg ccagattcag cttcgaccat accggttgcg gcagcgtgag
    11881 ttctttaccc agcatccgct gaattagcgg ctttttcatc acccattggc tgactaacag
    11941 ggcacccgca aacagggcat aaatgacagt aaccttccat ttaataaact catcattgtg
    12001 gaagaacagg gtcaagccac cgaagacgac caccagaaca aaagtgatca gggccatctt
    12061 ctcaacctta cgaaagcgaa cccagctata tataagcaca atcgccgtgg cgacgatcag
    12121 cgccgcagta gccgcataga tgtcataaat cttgtaaaac gcgaaaaaga caaccagcgg
    12181 taaaaaatca agaaactgct tcattttacg attccgtaat caaatgctta aggttattgg
    12241 cgaatcaaca tatacaggcg gaacagatag atgagcagta cggctgaaat caggttgctc
    12301 aaggtgttcg ccagtacggc accaatttcc ggggttaatg cggcaaaaga agaggcaaaa
    12361 agcagcaaca gtgtctttgc cagcaaccag ctcagtactg cgggtgccac cagacgcata
    12421 ttcgcccaag tcagccgcat actgctacgc atcgaggcaa aaacgcccat tttgtcctgt
    12481 accaacatca ccggtgccag ggccagtaaa atggccatga taattcccgg aacgaccacc
    12541 agcatgatgc caatctgtac taaaagggta gtcagaaaaa tcagaataaa taactttggc
    12601 aatatcggcg cactggcacc aatagctcgc agcgcactga ctctctgacc cgcagacacc
    12661 agctggataa ttaatattac gcctccggcg agaatggcgt taccgattaa tcctgaaaaa
    12721 gtggacgccg ctgaagcctg cagcaaaatt tgttgctgtt ccggtgacat attctgaacc
    12781 aggtcgaaca acccactact gccgctaacg ggcacgccgt cattgagctg cgcaagctgt
    12841 gcatcactgg gtgagaaaac atgccctaac accactgtga taaacgcaca tagcaacgat
    12901 accaacagaa tggtcataaa ttgattacgg aagaaatttc cggtgtcacg gtatacggac
    12961 tgcgccgtga tagacatgca ctctccttga gttttatgca ggtgttaatt agcgggtaat
    13021 tgtaccctgg ttaagcctcg ggtggcagca tcaggtcttg tatggaaaag catatctttg
    13081 taaagcggag gtaattgctg gccttcagac gtgggcaaat cgcggcaagt ggcgatcgag
    13141 ttaatatttg cgtagcgaaa atatttaaaa attgatttaa atcacattaa ccaggattct
    13201 caatgcaact tctaaattaa tccaga
//
LOCUS       DAZFLD010000133        13060 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.134, whole genome shotgun sequence.
ACCESSION   DAZFLD010000133 DAZFLD010000000
VERSION     DAZFLD010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13060)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13060)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13060
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.134"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1710
                     /locus_tag="ACHKBL_003297"
     CDS             <1..1710
                     /locus_tag="ACHKBL_003297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016241628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
                     /protein_id="HFO5820128.1"
                     /translation="FTHSLPMRYADFPTLVDALDYAALSSAGMNFYDRRCQLEDQLEY
                     QTLKARSEAGAKRLLSLNLKKGDRVALIAETSSGFVEAFFACQYAGLVAVPLAIPMGV
                     GQRDSWSAKLQGLLASCQPAAIITGDEWLPLVNAATHNNNPELHVLSHAWFKALPEAD
                     VALQRPVPNDIAYLQYTSGSTRFPRGVIITHHEVMANLRAISHDGIKLRPGDRCVSWL
                     PFYHDMGLVGFLLTPVATQLSVDYLRTQDFAMRPLQWLKLISKNRGTVSVAPPFGYEL
                     CQRRVNEKDLAELDLSCWRVAGIGAEPISAEQLHQFAECFRQVNFDDKTFMPCYGLAE
                     NALAVSFSDEACGVVVNEVDRDILEYQGKAVAPGAETRAVSTFVNCGKALPEHGIEIR
                     NEADIPVAERVVGHICISGPSLMSGYFGDQVSQDEIAATGWLDTGDLGYLLDGYLYVT
                     GRIKDLIIIRGRNIWPQDIEYIAEQEPEIHSGDAIAFVTAQEKIILQIQCRISDEERR
                     GQLIHALAARIQSEFGVTADIDLLPPHSIPRTSSGKPARAEAKKRYQKAYAASLHVQE
                     SLA"
     gene            1707..2621
                     /locus_tag="ACHKBL_003298"
     CDS             1707..2621
                     /locus_tag="ACHKBL_003298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001481799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFO5820129.1"
                     /translation="MNQTVAVTGATGFIGKYIIDNLLARGFHVRALTRTARAHVNDNL
                     IWVRGSLEDTHSLSELVAGASAVVHCAGQVRGHKEEIFTRCNVDGSLRLMQAAKESGF
                     CQRFLFISSLAARHPELSWYANSKHVAEQRLIAMADEITLGIFRPTAVYGPGDKELKP
                     LFDWMLRGLLPRLGAPDTQLSFLHVTDFAQAVGQWLNAETVQTQTYELCDGVAGGYDW
                     QRVRQLAAEARCGSVRMVGIPLPLLTCLADISTALSRLAGKEPMLTRSKIRELTHADW
                     SANNNRISEDINWFPGISLEHALRNGLF"
     gene            2653..2901
                     /locus_tag="ACHKBL_003299"
     CDS             2653..2901
                     /locus_tag="ACHKBL_003299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFO5820130.1"
                     /translation="MVNREIVMDYILSCLQDLVENGVEIKPDSDLVNDLGLESIKVMD
                     LLMMLEDRFDISIPINILLDVKTPAQLMETLLPWLENK"
     gene            2901..4073
                     /locus_tag="ACHKBL_003300"
     CDS             2901..4073
                     /locus_tag="ACHKBL_003300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFO5820131.1"
                     /translation="MGLYDKYARLAGERLQFSDNGLTPFGTCIDEVYSATEGRIGNKK
                     VILAGTNNYLGLTFNHDAIREGQAALAAQGTGTTGSRMANGSYAPHLALEKEIAEFFN
                     RPTAIVFSTSYTANLGVISALADHNAVVLLDADSHASIYDACSLGGAEIIRFRHNDVN
                     DLERRMVRLGERAKEAIIIVEGIYSMLGDVAPLAEIVDIKRRLGGYLIVDEAHSFGVL
                     GATGRGLVEAVGVEDDVDIIVGTFSKSLASIGGFAVGSEAMEVLRYGSRPYIFTASPS
                     PSCIATVRSSLRTISTQPELRQKLMDNANNLYDGLQKLGYELSSHISPVVPVIIGSKE
                     EGLRIWRELISLGVYVNLILPPAAPAGITLLRCSVNAAHSREQIDAIIQAFATLKQ"
     gene            complement(4108..5187)
                     /gene="lptG"
                     /locus_tag="ACHKBL_003301"
     CDS             complement(4108..5187)
                     /gene="lptG"
                     /locus_tag="ACHKBL_003301"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HFO5820132.1"
                     /translation="MNVFSRYLIRHLFLGFAAAAGLLLPLFTTFNLINELDDVSPGGY
                     RWTQAVLVVLMTLPRTLVELSPFIALLGGIVGLGQLSKNSELTAIRSTGFSIFRIALV
                     ALVAGILWTVSLGAIDEWVASPLQQQALQIKSTATALGEDDDITGNMLWARRGNEFVT
                     VKSLNEQGQPVDVEIFHYRDDLSLESYIYARSATIEDDKTWILHGVNHKKWLNGKETQ
                     ETSDNLPWQSAFTSMNLEELSMPGNTFSVRQLNHYIHYLQETGQPSSEYRLALWEKLG
                     QPILTLAMILLAVPFTFSAPRSPGMGSRLAVGVIVGLLTWISYQIMVNLGLLFALSAP
                     VTALGLPVAFVLVALSLVYWYDRQH"
     gene            complement(5184..6254)
                     /gene="lptF"
                     /locus_tag="ACHKBL_003302"
     CDS             complement(5184..6254)
                     /gene="lptF"
                     /locus_tag="ACHKBL_003302"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HFO5820133.1"
                     /translation="MKLVEHYIMRGTRRLVLIIVGFLIFIFASYSAQRYLTEAANGTL
                     ALDVVLDIVFYKVLIALEMLLPVGLYVSVGVTLGQMYTDSEITAISAAGGSPGRLYKA
                     VLYLAIPLSIFVTLLSMYGRPWAYAQIYQLEQQSQSELDVRQLRAKKFNTNDNGRMIL
                     SQTVDQDNNRLTDALIYTSTANRTRIFRARSVDVVDPSPEKPTVMLHNGTAYLLDHQG
                     RDDNEQIYRNLQLHLNPLDQSPNVKRKAKSVTELARSAFPADHAELQWRQSRGLTALL
                     MALLAISLSRVKPRQGRFSTLLPLTLLFVAIFYGGDVCRTLVANGAIPLIPGLWLVPG
                     LMLMGLLMLVARDFSLLQKFSR"
     gene            complement(6284..6844)
                     /locus_tag="ACHKBL_003303"
     CDS             complement(6284..6844)
                     /locus_tag="ACHKBL_003303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HFO5820134.1"
                     /translation="MSSRLIIALIIMLLAPGVQAHNFVTGKTVTPVYIQEGGELLRNS
                     DDEIHYQKWNSTQLAGKVRIIQYIAGRKSAKKKNSLLIKAVEAANFPQDRFQPTTIVN
                     TDDAIFGTGYFVVGKIEKNKRRYPWAQFVIDGNGQGRVAWHLQEQSSTILVLNKTGQI
                     QWAKDGSLTPEEVDHVIALAQKLINE"
     gene            complement(6856..7680)
                     /locus_tag="ACHKBL_003304"
     CDS             complement(6856..7680)
                     /locus_tag="ACHKBL_003304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5820135.1"
                     /translation="MMRKYFPLEASERLFVAIEEDDVVDAQVSLPPTIALSCTTEIIH
                     DNYALCLQFWLNGVNRQELLRLICKQAKGDELTADERKQFKYMRARYKHLRFAQRLYL
                     KKHQAGFLFGKTTVFLGRFQDGFRNGKKNIVSYYGNLLRVYLSSPVWSLVNYSLRHSQ
                     LESVSGFIAYRQKQMHTLKEIIAKPRLTGREFHDVRKIISQQVSYYDTLRSLDPENKE
                     ALQISRFLAAINGLMGDKHDDMVADDMENRQSYDAPMALDSDIRQRLELLISRFPV"
     gene            complement(7680..9029)
                     /locus_tag="ACHKBL_003305"
     CDS             complement(7680..9029)
                     /locus_tag="ACHKBL_003305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HFO5820136.1"
                     /translation="MAGFNIKHWFADGAFRTIIRNSAWLGSSNVVSALLGLLALSCAG
                     KGMTPAMFGVLVIVQSYAKSISDFIKFQTWQLVVQYGTPALTNNNPQQFRNVVSFSFS
                     LDIVSGAVAIVGGIALLPFLSHSLGLDDQSFWLAALYCTLIPSMASSTPTGILRAVDR
                     FDLIAVQQATKPFLRAAGSVVAWYFDFGFAGFVIAWYASNLVGGTMYWWFAARELRRR
                     NIHNAFKLNLFESARHIKGAWSFVWSTNIAHSIWSARNSCSTVLVGIVLGPAAAGLFK
                     IAMTFFDAAGTPAGLLGKSFYPEVMRLDPRTTRPWLLGVKSGLLAGGIGILVALAVLI
                     VGKPLISLVFGVKYLEAYDLIQVMLGAIVISMLGFPQESLLLMAGKQRAFLVAQTIAS
                     IGYIVLLFMFCHLFGVLGAAFAYFGGQCLDVVLSLIPTLKAFFQRHSLLYNAAGEKS"
     gene            complement(9075..9833)
                     /locus_tag="ACHKBL_003306"
     CDS             complement(9075..9833)
                     /locus_tag="ACHKBL_003306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5820137.1"
                     /translation="MDALQTQTVNSTTAPQPNYIPGLIAVVGCDGTGKSTLTTDLVKS
                     LQQHWQTERRYLGLLSGEDGDKIKRLPLVGVWLERRLAAKSSKTQSMKTKSPALWAAV
                     IMYCFSLRRMANLRKVQRLAQSGVLVVSDRFPQAEISGFYYDGPGIGVERATGKISMF
                     LAQRERRLYQQMAQYRPELIIRLGIDIDTAISRKPDHDYAELQDKIGVMSTIGYNGTK
                     ILEIDSRAPYSEVLEQAQKAVSLVAIVSDRRSLT"
     gene            complement(9865..10578)
                     /gene="yghS"
                     /locus_tag="ACHKBL_003307"
     CDS             complement(9865..10578)
                     /gene="yghS"
                     /locus_tag="ACHKBL_003307"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006235 - dTTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFO5820138.1"
                     /translation="MNQRNMSIINSTPVRVIAIVGCDGSGKSTLTASLVNELAATMPT
                     EHIYLGQSSGRIGEWISQLPVIGAPFGRYLRSKAAHVHEKPSTPPGNITALVIYLLSC
                     WRAYKFRKMLCKSQQGYLLITDRYPQVEVPGFRFDGPQLAKTTGGNGWIKMLRQRELK
                     LYQWMASYLPVLLIRLGIDEQTAFARKPDHQLAALQEKIAVTPQLTFNGAKILELDGR
                     QPADEILQASLRAIHAALS"
     gene            10752..11444
                     /gene="yghT"
                     /locus_tag="ACHKBL_003308"
     CDS             10752..11444
                     /gene="yghT"
                     /locus_tag="ACHKBL_003308"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006235 - dTTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFO5820139.1"
                     /translation="MQSITPPLIAVIGSDGSGKSTVCEHLITVVEKYGAAERVHLGKQ
                     AGNVGRAVTKLPLMGKSLHKTIEKNEVKTAKKLPGPVPALVITAFVARRLLRFRHMLA
                     CRRRGLIVLTDRYPQDQIPGAYDGTVFPPNVEGGRFVSWLASQERKAFHWMASHKPDL
                     VIKLNVDLEVACARKPDHKRESLARKIAITPQLTFGGAQLVDIDANQPLEKVLVDAEK
                     AITDFMTARGYH"
     gene            complement(11493..12992)
                     /gene="pitB"
                     /locus_tag="ACHKBL_003309"
     CDS             complement(11493..12992)
                     /gene="pitB"
                     /locus_tag="ACHKBL_003309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitB"
                     /protein_id="HFO5820140.1"
                     /translation="MLNLFVGLDIYTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMQPQLAVVMAAFFNFFGVLLGGLSVAYAIVHMLPTDLLLNMGSTHGLAMVFSMLL
                     AAIIWNLGTWFFGLPASSSHTLIGAIIGIGLTNALLTGSSVMDALNLREVTKIFSSLI
                     VSPIVGLVIAGGLIFLLRRFWSGTKKRDRIHRIPEDRKKKKGKRKPPFWTRIALIVSA
                     AGVAFSHGANDGQKGIGLVMLVLVGIAPAGFVVNMNASGYEITRTRDAVTNFEHYLQQ
                     HPELPQKLIAMEPPLPAASTDGTQVTEFHCHPANTFDAIARVKTMLPGNMESYEPLSV
                     SQRSQLRRIMLCISDTSAKLAKLPGVSKEDQNLLKKLRSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVAMTIGEKIGKRGMTYAQGMAAQMTAAVSIGLASYIGMPVSTTHVL
                     SSAVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAIFLSGGLYWIALQLI"
BASE COUNT         3304 a         3404 c         3238 g         3114 t
ORIGIN      
        1 tttacgcatt ccctacctat gcgctatgcc gattttccaa cgctggttga tgctttggac
       61 tacgccgctc tgagtagcgc cggaatgaat ttttatgaca gacgttgcca acttgaagat
      121 caactggaat atcagacgtt aaaagcgcgt tccgaagctg gtgcgaaaag gttgttatcg
      181 ctgaacctga aaaaaggcga tcgcgtggcg ctgattgccg aaacgagtag cgggtttgta
      241 gaggcttttt ttgcctgcca gtatgccggc ttagtcgccg tcccgttggc gattccaatg
      301 ggcgtcggtc agcgggattc ctggagcgcc aaactgcagg gtttactggc aagttgccag
      361 cccgcagcca ttatcactgg tgatgagtgg ttgccactgg tcaatgccgc gacgcataac
      421 aacaaccccg aattacatgt tttaagccac gcctggttta aggcattacc ggaagccgat
      481 gttgcgctcc agcgtccagt tccaaacgat atcgcctacc tccagtacac ctccggcagc
      541 acccgttttc cccgtggcgt cattatcacc catcacgaag tgatggctaa tctacgtgct
      601 ataagccacg atggcattaa attacgccct ggcgaccgct gcgtctcctg gctgcctttc
      661 taccatgata tgggactggt cggctttctc ctgacccccg tcgccacgca gctttcagta
      721 gattatttgc gcactcagga ttttgccatg cgtcctctgc aatggcttaa attgatcagt
      781 aaaaatcgcg gcaccgtttc cgttgcaccg ccgtttggct atgaattgtg ccagcgccgc
      841 gtgaatgaaa aagatctcgc tgaactggat ctttcctgct ggcgcgtcgc tggtattgga
      901 gcagaaccga tctccgcaga acaactccat caattcgctg aatgttttcg tcaggttaac
      961 tttgacgata aaactttcat gccgtgctac ggactggcag aaaatgcgct ggctgtcagc
     1021 ttctctgatg aagcctgcgg ggttgtggtt aacgaagtgg atcgcgacat cctcgaatac
     1081 cagggtaaag ccgtcgcgcc gggtgctgaa acacgcgccg tatcgacttt cgtcaactgc
     1141 ggtaaagcgt tgccggaaca tggcattgaa atccgcaatg aagcagatat acctgtcgcg
     1201 gaacgtgtgg taggccatat ttgcatctcc ggccccagcc tgatgagcgg gtactttggc
     1261 gaccaggttt cgcaagacga gattgccgcg acgggctggt tagacaccgg cgaccttggt
     1321 tatctgctgg acggttatct gtatgtcacc ggacgcatta aagatctgat tattattcgt
     1381 ggccgtaata tctggccgca ggatattgaa tacattgcgg aacaggaacc ggaaattcat
     1441 tctggcgatg cgattgcttt tgttaccgcc caggaaaaaa tcattttgca gatccagtgt
     1501 cggatcagcg acgaagaacg tcgcgggcag cttatccacg cgctggcagc tcggatccaa
     1561 agcgaatttg gcgttaccgc ggatatcgat ctgttgccgc cccacagtat tccccgaaca
     1621 tcctccggca agcctgcccg tgcggaagcg aaaaaacgtt atcagaaggc ttatgctgcc
     1681 agtcttcatg tgcaggaatc cctggcatga atcaaactgt cgcggtgacg ggcgcaaccg
     1741 ggtttatcgg taaatatatt attgataacc tgctcgcccg cggctttcac gttcgcgcat
     1801 taacgcgtac tgcccgcgct cacgtcaatg ataatctcat ctgggtgcgc ggttcgctgg
     1861 aagatacaca ttcgcttagc gaactggttg ccggagccag cgcggtggtc cattgcgccg
     1921 gacaagtgcg cggacacaaa gaagagattt ttacccgctg taacgttgac ggcagcctgc
     1981 gcctgatgca agcggcaaaa gagagcggct tttgccaacg ttttctgttt atctcttcgc
     2041 tggcggcgcg ccatcccgaa ctctcctggt acgcaaattc caaacacgtc gccgaacaac
     2101 gactgatcgc aatggctgac gaaattacgc tgggcatttt tcgcccgaca gccgtgtatg
     2161 gtcccggcga taaagagtta aaaccgctgt ttgactggat gctgcgcggc ctgctgccac
     2221 gacttggtgc gccagataca caactctctt tcctgcacgt caccgatttt gcgcaagcag
     2281 tgggtcagtg gttaaacgcc gaaactgtac agacgcaaac ctatgaatta tgcgatggcg
     2341 tcgctggcgg ctatgactgg caacgcgtac gacagcttgc cgccgaagcc cgttgtggtt
     2401 ctgtgcggat ggttggcatt cctctgccgc tactcacctg ccttgcggat atcagtactg
     2461 cgttgagtcg cctggcaggt aaagaaccta tgctgacccg ctcgaaaatt cgtgaattaa
     2521 cccacgccga ctggtcggca aataataacc gtatttctga agatattaat tggtttcccg
     2581 ggattagcct ggaacacgca ttacgcaacg ggctattttg attgtcttgt ttaaaggtgt
     2641 gttaacgttt ctatggtaaa tcgtgaaata gtaatggatt acatcctttc ctgtttacag
     2701 gatttggtcg aaaacggtgt ggaaattaag cccgacagcg atctcgtcaa cgatcttggc
     2761 ctggagtcga tcaaagtgat ggatctctta atgatgctgg aagacagatt cgatatttcc
     2821 attcctatta atattctgct ggatgtcaaa acccctgcgc aattaatgga aactctactt
     2881 ccatggctgg agaataaata atggggctat acgataaata tgcgcgcctt gctggcgagc
     2941 gcctgcaatt tagtgacaac gggttaacac catttggcac ctgtattgat gaagtgtatt
     3001 ccgccaccga aggccggatc ggcaataaaa aagtgattct ggccgggacc aataattatc
     3061 tcggtttaac ctttaatcat gatgctatcc gcgaaggtca ggcagcgtta gcggcccaag
     3121 gcactggcac caccggttct cgtatggcaa acggcagtta tgcgccgcat ctggcgttag
     3181 agaaagagat tgccgagttt ttcaatcgcc ctaccgctat cgttttttca accagttata
     3241 ccgctaattt aggtgtcatc agcgcacttg ccgaccataa cgccgtggtg ttactggatg
     3301 ccgacagcca cgccagtatt tatgacgcct gttctctcgg cggcgcagag attattcgtt
     3361 tccgccataa tgacgtcaac gatctggaac ggcgcatggt ccgcctcggt gaacgggcga
     3421 aagaggcgat tattatcgtt gaaggcatct acagtatgct cggtgacgtt gcgccgctgg
     3481 cagaaattgt tgatatcaaa cgtcgtctcg gtggttattt gattgtcgat gaagcacatt
     3541 cgtttggcgt gttaggcgca acaggccgcg gactggttga agccgttggc gttgaagacg
     3601 acgtggatat tatcgtcggc accttcagta aaagtttggc ttccattggt ggttttgccg
     3661 tgggttcaga agcgatggaa gtgctgcgct acggcagccg cccgtatatt tttaccgcct
     3721 ctccttctcc gtcctgcatt gcgaccgtgc gctcgtcgtt aagaaccatt tccacacaac
     3781 cagagctgcg gcaaaaactg atggataacg ccaacaatct gtatgacggt ctgcaaaaac
     3841 tgggttatga gttaagttca catattagcc cggtagtgcc agtaataatc ggttcgaaag
     3901 aggaaggttt acgcatctgg cgcgagctta tttcgctggg cgtttatgtg aatcttatcc
     3961 tgccacctgc cgcgcctgca ggcatcacgc tgctgcgctg tagcgtaaac gctgcccaca
     4021 gtcgtgagca aatcgatgcc atcattcagg catttgctac tctgaagcag taatgtgagc
     4081 ccgctgtggt gttgcgccac ggcgggttta atgttgtctg tcataccaat acaccaggct
     4141 caacgccacc agcacaaacg ctaccggtaa accgagcgcg gtaacaggtg cgctcaacgc
     4201 aaataacaat cccagattga ccatgatttg atagctgatc caggtgagta agccaacgat
     4261 gacgcctaca gcgagacggc tacccatccc aggcgaacgc ggggcgctaa aggtaaacgg
     4321 cacagccagc aaaatcatcg ccagggtcaa gatgggttgt cccagttttt cccacagtgc
     4381 aaggcggtat tcgctgctgg gttgcccggt ttcctgcaaa taatggatgt aatgattaag
     4441 ttgacggaca gaaaaagtat ttcccggcat cgataactct tcaagattca tactggtgaa
     4501 ggccgattgc cagggaagat tatcagatgt ttcctgcgtt tctttgccat tcagccattt
     4561 tttatgattc acaccatgca ggatccacgt tttgtcgtct tcaatggtag cactgcgcgc
     4621 ataaatgtag gattcgagcg agagatcatc gcggtaatga aatatctcca cgtccaccgg
     4681 ctggccctgc tcgttcagcg atttcaccgt cacaaattca ttaccacgcc tggcccacag
     4741 catattgccg gtaatgtcat cgtcctcccc caacgcagtg gcagtcgatt tgatttgcag
     4801 cgcctgttgc tgcaatggcg acgccaccca ctcatctatc gcgcctaatg aaacagtcca
     4861 caatatcccg gcaaccagcg ccaccagtgc aatacggaag atagaaaacc ccgtgctgcg
     4921 aatggcggta agctcactgt ttttcgataa ctgccccagg ccgacaatcc ctcccaataa
     4981 ggcgataaac ggcgaaagtt cgaccagtgt gcgcggtaag gtcatcagca ccaccagcac
     5041 cgcctgagtc cagcgataac cacccgggct gacatcatcc agttcgttaa tcaggttgaa
     5101 ggtggtaaaa agcggcagca atagccctgc ggcggcggca aaaccgagaa agagatgacg
     5161 gattaaatag cgactgaaaa cattcatcgg gaaaatttct gcagcaaaga gaagtcgcgt
     5221 gcgaccaaca tcagcaggcc cattagcatg agtccgggta ctaaccacaa accaggaatg
     5281 aggggaatcg cgccgttagc caccagcgta cggcagacgt cgccgccata aaaaatggca
     5341 acaaacagca acgtcagtgg caataacgtt gaaaatcgcc cttgccgcgg ttttacccga
     5401 cttaatgaaa tggccagcag cgccatcaac aatgctgtca ggccacggct ttgtcgccat
     5461 tgcagttcgg catgatcggc aggaaaggcg gagcgcgcca gctccgtgac cgattttgct
     5521 ttgcgtttga cgttagggct ttgatccagc ggattcagat gtaattgcag attacggtag
     5581 atctgttcgt tgtcgtcacg cccctgatga tcgagaagat aggcggtccc gttatgcaac
     5641 ataacggtcg gtttctcagg tgatgggtca accacatcaa ccgaacgggc gcggaaaatg
     5701 cgggttcgat tagcagtaga agtataaatc agcgcatcag tcaggcgatt attatcctga
     5761 tcaaccgtct gcgaaaggat cattcgtccg ttatcgttag tgttaaattt ctttgcccgc
     5821 aactggcgaa catccagctc cgactgtgac tgttgctcca gttgataaat ctgcgcataa
     5881 gcccacggac gaccatacat cgacagaagg gtgacaaaaa tacttagcgg tatcgccaga
     5941 taaagaacgg ctttgtacaa gcgtcccgga ctgccccccg ccgcagagat agcggtaatt
     6001 tccgagtcgg tatacatctg ccctagcgtc acgccaactg acacatacag tccaacaggt
     6061 aacaacatct ccagtgcaat cagcactttg tagaaaacga tatccagcac aacatcaaga
     6121 gctaatgtcc catttgccgc ttcggtcaga taacgctgtg cggagtagct ggcgaaaata
     6181 aagatcagaa aaccgacgat aatcagcacc agacggcgcg taccacgcat gatgtagtgt
     6241 tcaaccagct tcatctcact aacgccctca atcctttgaa tttttattca ttgatcagct
     6301 tctgcgccag cgcaatgaca tgatctacct cttccggcgt caggctgccg tcctttgccc
     6361 actgaatttg cccggtttta ttcagcacca gaatggttga actctgctcc tgcaagtgcc
     6421 aggcaacgcg tccctgtcca ttgccgtcaa taacgaattg cgcccatgga tagcggcgtt
     6481 tatttttttc gattttgcca acgacaaaat agccggtacc aaagatggca tcatcagtgt
     6541 tcacaatggt ggtgggctga aaacgatcct gcggaaagtt tgccgcttca actgctttaa
     6601 ttaacagtga gttctttttc ttcgccgact tacgcccggc gatatactga ataatgcgaa
     6661 cttttcccgc caattgggtg ctgttccact tttggtagtg aatttcgtcg tcgctattcc
     6721 gaagcagctc gccgccttcc tggatataaa cgggggtaac cgttttgcca gtgacgaaat
     6781 tgtgtgcctg cacacccggg gctaagagca tgataatcag cgcaataatc aaacgagatg
     6841 acatcattgc tccggttata ccggaaaacg cgaaatcagc agttctaagc gctggcgaat
     6901 atcgctatcc agcgccatcg gtgcatcata cgactggcgg ttttccatat catccgccac
     6961 catgtcgtcg tgtttatccc ccatcaggcc attaatggcg gcgaggaagc gggaaatctg
     7021 taacgcttct ttattttcag gatcaagcga tcttagggtg tcgtaataag aaacctgctg
     7081 gctgatgatt tttcgcacat catgaaattc cctgccggtt agtcgcggtt tggcaattat
     7141 ctctttcagg gtatgcattt gcttttgccg ataggcaata aaaccgctta cgctttctaa
     7201 ctggctatgg cgtaatgaat agttcaccaa cgaccagacc ggagagctta aataaacccg
     7261 taacaggttg ccgtaatacg acacaatatt tttcttaccg ttacgaaagc cgtcctgaaa
     7321 acgccccaaa aaaacggtcg ttttgccgaa taaaaatccg gcctgatgct tctttaaata
     7381 cagccgttgc gcaaaacgca ggtgcttgta gcgcgctcgc atgtatttaa attgtttgcg
     7441 ctcatcagca gtcagctcat cgcctttggc ctgtttgcat attaaacgca gtaactcctg
     7501 gcgattgacg ccattgagcc agaattgcag gcataacgcg taattatcat ggatgatttc
     7561 ggtggtacaa cttagtgcga tagtcggcgg caaactgacc tgtgcatcaa cgacatcatc
     7621 ttcttcaatc gcaacaaata gacgctcgct cgcctctagc gggaaatatt ttctcatcat
     7681 caggatttct ctccggctgc gttataaagc aaggaatggc gctgaaaaaa tgcctttaag
     7741 gtcggaatta gcgaaagcac cacatccaga cattgaccac cgaaataagc aaacgctgct
     7801 cccagcacgc caaacagatg acagaacatg aacagcaata cgatgtagcc gattgaggcg
     7861 atggtttgcg ccacgagaaa agcgcgctgt tttcccgcca tcaatagcaa tgactcctga
     7921 gggaagccca gcatcgagat cacaattgcg cccagcatca cctgaatcag gtcatacgct
     7981 tcgagatact taacgccaaa caccagcgaa atgagcggct tgccgacaat caacaccgcg
     8041 agcgccacca gaataccgat tccgcccgcc agtaaacctg acttcacgcc taacaaccac
     8101 ggtctggtgg tacgcggatc taaacgcatc acctctgggt aaaaactttt gcccagtaaa
     8161 cccgctggcg ttccggcggc gtcgaagaat gtcatggcga ttttaaataa cccggcggca
     8221 gcgggtccta acacgatccc caccaacact gtgctgcacg aattacgcgc cgaccagatg
     8281 gaatgggcaa tgttggttga ccagacaaaa ctccacgcgc ctttgatgtg tcgggcagac
     8341 tcaaacagat tcaatttgaa cgcgttatgg atatttctgc ggcgtaattc acgtgcggca
     8401 aaccaccagt acatggtgcc gccaacaaga ttcgaggcgt accaggcaat aacaaaaccc
     8461 gcaaaaccaa agtcaaaata ccaggcaacg acgctccccg ctgcgcgcag aaaaggtttc
     8521 gtcgcttgct gtacagcaat tagatcgaag cgatctaccg cacgcagaat gccggtcggc
     8581 gtggaggaag ccattgaagg aatgagcgtg caatacagcg ctgccagcca aaagctttgg
     8641 tcatccagac ctaatgaatg ggaaagaaaa gggagtaagg caatgccgcc gacaatcgcc
     8701 accgcgccgc tgacgatatc cagcgagaag gaaaatgaga cgacattgcg gaattgctgc
     8761 ggattattgt tggttaatgc tggtgttccg tactgaacca ccagctgcca tgtctgaaac
     8821 ttaataaaat cgctgatcga cttggcgtac gattgtacaa tcaccagtac gccaaacatg
     8881 gcgggcgtca tccctttacc ggcacacgag agcgccaaca gacccagcaa agcgctcacg
     8941 acattactgg agcctaacca ggcgctattg cgaataatgg tgcgaaacgc gccatctgca
     9001 aaccaatgtt tgatgttaaa acccgccaat tcagcctgac cttatcgttg atagtaaaaa
     9061 gtatcccgcc agccttaagt taaacttcgg cggtcagaaa cgatggcaac cagagaaacc
     9121 gccttctgtg cctgttccag cacttcgctg tagggcgctc tggaatcaat ctcaagaatt
     9181 tttgtgccgt tatagccaat cgtcgacatg acgccgattt tgtcctgcag ctcggcatag
     9241 tcatggtcag gcttgcggga gatggcagta tcaatatcaa tccccaaacg aatgattaat
     9301 tcaggacggt attgcgccat ttgttggtat aaacgccgtt cgcgctgcgc cagaaacatg
     9361 ctgattttcc cggtcgcacg ttcgacgccg atccccggtc cgtcataata aaagcccgaa
     9421 atttcagcct gcgggaagcg atcgctgacc accagaacgc cactttgcgc cagtcgctga
     9481 accttgcgta gattcgccat tcttcgcagc gagaagcagt acataatcac cgccgcccat
     9541 agcgctggag atttggtttt catgctttgg gttttcgagg atttggccgc cagtcgccgt
     9601 tcgagccaga cgccaaccaa cggcaatcgt ttgattttgt cgccgtcttc gcccgagagc
     9661 agccccagat agcgccgctc ggtttgccag tgttgttgca gcgatttcac caggtcggtg
     9721 gtcagtgtgg atttaccggt gccatcacac ccgaccaccg caatcagccc cggaatgtag
     9781 ttgggctgcg gcgcggttgt actattaaca gtttgtgttt gtagtgcgtc cattcgttaa
     9841 atcatcccct atccattttg gtgatcagga aagcgcggcg tgaattgcgc gtagtgacgc
     9901 ttgcagaatt tcatcggcgg gttgccgacc atccagttca aggatctttg cgccattaaa
     9961 tgtcagttgc ggcgtaacgg cgattttttc ctgtaacgcc gccagttggt ggtcaggttt
    10021 acgcgcaaac gcggtttgtt catcaatgcc aagacgaatc aacaatacgg gcaaataaga
    10081 cgccatccat tggtacagct tcagctcgcg ctgccttaac atttttatcc aaccgttacc
    10141 gcccgtggtt tttgccaatt gcgggccatc aaagcgaaaa cccggcactt caacctgcgg
    10201 gtagcggtcg gtgatgagca gatagccttg ctggctttta cacaacattt tgcgaaactt
    10261 gtacgcccgc cagcaggaaa gcagatagat aactagcgca gtaatattgc caggtggtgt
    10321 ggagggcttt tcgtgcacat gtgccgcttt acttcgcaga taacgcccaa aaggtgcgcc
    10381 aataacaggg agctgtgaaa tccattcgcc aattcgcccg gacgattgcc cgagataaat
    10441 gtgttctgtt ggcattgttg ctgccagttc atttaccagg cttgccgtga gggtcgattt
    10501 acctgaacca tcacatccta caatggcaat aacacgcact ggcgtggaat taattattga
    10561 catattacgt tgattcacga aaaacccggc agcaaataat gtattgaata ttacgtggtc
    10621 cgtgctcaga atatccgttc aaccttattg tgacaatggt taattgtgac attaacctga
    10681 attacgcttt cataaaaaca tattaatcaa ataaatattt tggatggata tttaaattta
    10741 aggatatatt catgcagtca ataacacctc cattaattgc cgttattggt agcgatggtt
    10801 caggcaagtc aacggtgtgt gaacatctta ttaccgttgt cgaaaaatat ggtgctgccg
    10861 aaagagttca tttaggaaaa caggccggaa atgtcggtcg tgcagtgaca aaattaccgt
    10921 tgatgggaaa gtctctgcac aagactattg aaaaaaatga agtgaaaacg gcaaaaaaat
    10981 tgccgggtcc ggttccggcc ctggtgatca ccgctttcgt cgcccgccga ctgttacgct
    11041 ttcgtcatat gctcgcctgt cgtcgtcgcg ggctgattgt cttaaccgat cgttatcctc
    11101 aggaccaaat tcctggcgct tacgatggta cggtgttccc acctaacgtt gaaggcggcc
    11161 ggtttgtctc atggctggca agccaggaac gtaaagcatt tcactggatg gcgagccata
    11221 agcctgatct ggtcatcaaa ctcaatgttg accttgaagt tgcctgcgcg cgtaaacccg
    11281 accataaacg ggaatcgctg gcgaggaaga ttgccataac gccacagtta acctttggcg
    11341 gtgcacaact ggttgatatc gacgccaatc agccactgga aaaggtgctg gttgatgcag
    11401 aaaaagcgat tacggatttt atgaccgcgc gtggttatca ctagtcaaaa atggaaatgc
    11461 ccgatcgcca ggaccgggca ttttcaggaa ggttaaatca actgcaatgc tatccagtac
    11521 aacccaccag aaagaaaaat cgccgccggt aaagtaaaca cccacgccat caggatgctg
    11581 gttaccgttt tacgctgtaa cccaccgccg tccaccacca tcgtccctgc taccgcagac
    11641 gagaggacgt gtgttgtgga gacgggcatc ccaatataac tggcaaggcc gatagacact
    11701 gccgccgtca tttgtgccgc catgccttgc gcatacgtca tgccgcgctt accaatcttc
    11761 tcaccgatgg tcatcgctac acgacgccag ccaatcatgg tgccaatgcc gagcgccaga
    11821 gctactgcca tgatgatcca caccggagcg tactcaatgg tgcttaacat atcgctgcgc
    11881 agttttttca gcaggttctg gtcttcttta ctgacgcctg gcagtttcgc cagcttcgcg
    11941 gaggtatcag agatgcacag cataatgcgg cgcagctggc tgcgctgact cacgcttaac
    12001 ggctcgtaac tttccatatt gcctggcagc atcgttttaa cgcgcgcaat cgcatcaaag
    12061 gtatttgccg gatgacagtg aaactccgtt acttgcgtgc catcagtcga tgccgcaggc
    12121 aatggaggtt ccatcgcaat caacttctgc ggcagttcag gatgctgttg caggtagtgt
    12181 tcgaagttgg tgacggcatc gcgggtacgg gtaatttcat agccggacgc attcatattg
    12241 acgacgaaac cagcaggggc aatccccacc agtaccagca tcaccaggcc gatccctttc
    12301 tgcccgtcgt tagcaccgtg cgaaaacgcc acgcccgcag cggaaacaat cagcgcaata
    12361 cgcgtccaga atggtggctt acgtttgcct tttttctttt tgcgatcttc cggaatgcgg
    12421 tgaatacggt cacgcttttt cgtcccgctc cagaaacggc gcagcaggaa aatcagacca
    12481 cccgcaatga ccaggccgac gataggggaa acaatcagcg aggagaaaat tttggtcact
    12541 tcacgcaggt ttaacgcatc catcaccgat gagccggtta acagcgcgtt ggttaaaccg
    12601 atgccgataa tcgcaccaat caaggtgtgc gaactggagg ccggtaaacc aaagaaccac
    12661 gttcccaggt tccagataat cgcggccagc agcatggaaa agaccatcgc caggccgtga
    12721 gttgacccca tattcagcaa caaatcggtt ggtaacatat ggacaatggc ataggcaacg
    12781 ctaagtccgc ctagcaatac gccaaaaaag ttaaaaaatg ccgccatcac cacggcaagc
    12841 tgtggctgca tggctcgagt ataaataacg gttgccaccg cattcgccgt gtcatgaaaa
    12901 ccattgattg cttcgtagaa caacacaaat gccagagcaa gtaataacaa aagccctgtg
    12961 tatatatcaa ggccaacaaa taaatttagc atatattaga ttacgccatt ttgaatttac
    13021 gaacggacgc attatcagtg actttaacgg catgggcaaa
//
LOCUS       DAZFLD010000134        12854 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.135, whole genome shotgun sequence.
ACCESSION   DAZFLD010000134 DAZFLD010000000
VERSION     DAZFLD010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12854)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12854)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12854
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.135"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            127..1185
                     /gene="citC"
                     /locus_tag="ACHKBL_003310"
     CDS             127..1185
                     /gene="citC"
                     /locus_tag="ACHKBL_003310"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008771 - [citrate (pro-3S)-lyase] ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006101 - citrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HFO5820141.1"
                     /translation="MFGNDIFTRVKRSENKKMAEIAQFLHENDLSVDTTVEVFITVTR
                     DEKLIACGGIAGNIIKCVAISESVRGEGLALTLATELINLAYERHSTHLFIYTKTEYE
                     ALFRQCGFSTLTSVPGVMVLMENSATRLKRYAESLKKFCHPGNKIGCIVMNANPFTNG
                     HRYLIQQAAAQCDWLHLFLVKEDSSRFPYEDRLDLVLKGTADIPRLTVHRGSEYIISR
                     ATFPCYFIKEQSVINHCYTEIDLKIFRQYLAPALGVTHRFVGTEPFCRVTAQYNQDMR
                     YWLETPTISAPPIELVEIERLRYQEMPISASRVRQLLAKNDLTAIAPLVPAVTLHYLQ
                     NLLEHSRQDAAARQKTPA"
     gene            1200..1496
                     /gene="citD"
                     /locus_tag="ACHKBL_003311"
     CDS             1200..1496
                     /gene="citD"
                     /locus_tag="ACHKBL_003311"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HFO5820142.1"
                     /translation="MKINQPAVAGTLESGDVMIRIAPLDTQDIDLQINSSVEKQFGDA
                     IRTTILDVLARYNVRGVQLNVDDKGALDCILRARLEALLARASGIPALPWEDCQ"
     gene            1493..2401
                     /gene="citE"
                     /locus_tag="ACHKBL_003312"
     CDS             1493..2401
                     /gene="citE"
                     /locus_tag="ACHKBL_003312"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006084 - acetyl-CoA metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HFO5820143.1"
                     /translation="MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLE
                     DSVALREKDTARRMVYHALQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGADVVRL
                     PKTDTAQDVLDIEKEILRIEKACGREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
                     ALGAEDYVRNLRTERSPEGTELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEA
                     AHIKQLGFDGKSLINPRQIDLLHNLYAPTQKEVDHARRVVEAAEAAAREGLGVVSLNG
                     KMVDGPVIDRARLVLSRAELSGIREE"
     gene            2412..3944
                     /gene="citF"
                     /locus_tag="ACHKBL_003313"
     CDS             2412..3944
                     /gene="citF"
                     /locus_tag="ACHKBL_003313"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008814 - citrate CoA-transferase activity
                     [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HFO5820144.1"
                     /translation="MTQKIEQSQRQERVAAWNRRAECDLAAFQNSPKQTYQAEKARDR
                     KLCANLEEAIRRSGLQDGMTVSFHHAFRGGDLTVNMVMDVIAKMGFKNLTLASSSLSD
                     CHAPLVEHIRQGVVTRIYTSGLRGPLAEEISRGLLAEPVQIHSHGGRVHLVQSGELNI
                     DVAFLGVPSCDEFGNANGYSGKACCGSLGYAMVDADNAKQVVMLTEELLPYPHNPASI
                     EQDQVDLIVKVDRVGDAAKIGAGATRMTTNPRELLIARSAADVIVNSGYFKEGFSMQT
                     GTGGASLAVTRFLEDKMRSRDIRADFALGGITATMVYLHEKGLIRKLLDVQSFDSHAA
                     QSLARNPNHIEISANQYANWGSKGAAVDRLDVVVLSALEIDTQFNVNVLTGSDGVLRG
                     ASGGHCDTAIASALSIIVAPLVRGRIPTLVDNVLTCITPGSSVDILVTDHGIAVNPAR
                     PELAERLQEAGIKVVSIEWLRERARLLTGEPQPIEFTDRVVAVVRYRDGSVIDVVHQV
                     KE"
     gene            3948..4499
                     /gene="citX"
                     /locus_tag="ACHKBL_003314"
     CDS             3948..4499
                     /gene="citX"
                     /locus_tag="ACHKBL_003314"
                     /EC_number="2.7.7.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050519 - holo-citrate lyase synthase
                     activity [Evidence IEA];
                     GO_process: GO:0051191 - prosthetic group biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HFO5820145.1"
                     /translation="MHLLPELASHHAVSIPELLVSRDERQARQHVWLKRHPVPLVSFT
                     VVAPGPIKDSEVTRRIFNHGVTALRALAAKLGWQIQEQAALVSASGPEGMLSIAAPAR
                     DLKLATIELEHSHPLGRLWDIDVLTPEGEILSRRDYSLPPRRCLLCEQSAAVCARGKT
                     HQLTDLLNRMEALLNDVDACNVN"
     gene            4474..5352
                     /gene="citG"
                     /locus_tag="ACHKBL_003315"
     CDS             4474..5352
                     /gene="citG"
                     /locus_tag="ACHKBL_003315"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HFO5820146.1"
                     /translation="MSMPATSTKNTKLATSLIDEYALLGWRAMLTEVNLSPKPGLVDR
                     INCGAHKDMALEDFHRSALAIQGWLPRFIEFGACSAEMAPEAVLNGLRPIGMACEGDM
                     FRATAGVNTHKGSIFSLGLLCAAIGRLLQLNQPVTPTTVCSTAASFCRGLTDRELRTN
                     NSRLTAGQRLYQQLGLTGARGEAEAGYPLVINHALPHYLTLLDQGLDPELALLDTLLL
                     LMATNGDTNVASRGGEGGLRWLQREAQTLLQKGGIRTPADLDYLRQFDRECIERNLSP
                     GGSADLLILTWFLAQI"
     gene            5403..6866
                     /gene="citT"
                     /locus_tag="ACHKBL_003316"
     CDS             5403..6866
                     /gene="citT"
                     /locus_tag="ACHKBL_003316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/succinate antiporter CitT"
                     /protein_id="HFO5820147.1"
                     /translation="MSLAKDNIWKLLAPLVVMGVMFLIPVPDGMPPQAWHYFAVFVAM
                     IVGMILEPIPATAISFIAVTICVIGSNYLLFDAKELADPAFNAQKQALKWGLAGFSST
                     TVWLVFGAFIFALGYEVSGLGRRIALFLVKFMGKRTLTLGYAIVIIDILLAPFTPSNT
                     ARTGGTVFPVIKNLPPLFKSFPNDPSARRIGGYLMWMMVISTSLSSSMFVTGAAPNVL
                     GLEFVSKIAGIQISWLQWFLCFLPVGVILLIIAPWLSYVLYKPEITHSEEVATWAGDE
                     LKTMGALTRREWTLIGLVLLSLGLWVFGSEVINATAVGLLAVSLMLALHVVPWKDITR
                     YNSAWNTLVNLATLVVMANGLTRSGFIDWFANTMSTHLEGFSPNATVIVLVLVFYFAH
                     YLFASLSAHTATMLPVILAVGKGIPGVPMEQLCILLVLSIGIMGCLTPYATGPGVIIY
                     GCGYVKSKDYWRLGAIFGVIYISMLLLVGWPILAMWN"
     gene            6980..7786
                     /gene="rna"
                     /locus_tag="ACHKBL_003317"
     CDS             6980..7786
                     /gene="rna"
                     /locus_tag="ACHKBL_003317"
                     /EC_number="4.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease I"
                     /protein_id="HFO5820148.1"
                     /translation="MKAFWRNAALLAVSLLPFSSANAVALQAKQYGDFDRYVLALSWQ
                     TGFCQSQHDRNRNERDECRLQTETTNKADFLTVHGLWPGLPKSVAARGVDERRWMRFG
                     CATRPIPNLPEARASRMCSSPETGLSLETATKLSEVMPGAGGRSCLERYEYAKHGACF
                     GFDPDAYFGTMVRLNQEIKESEAGKFLADNYGKTVSRRDFDAAFAKSWGKENVKAVKL
                     TCQGNPAYLTEIQISVKADAINAPLSANSFLPQPHPGNCGKTFVIDKAGY"
     gene            8016..8426
                     /gene="rnk"
                     /locus_tag="ACHKBL_003318"
     CDS             8016..8426
                     /gene="rnk"
                     /locus_tag="ACHKBL_003318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HFO5820149.1"
                     /translation="MSRPTIIINDLDAERIDILLEQPAYAGLPIADALNAELDRAQMC
                     SPEEMPHDVVTMNSRVKFRNLSDGEVRVRTLVYPAKMTDSNTQLSVMAPVGAALLGLR
                     VGDSIHWELPGGVATHLEVLELEYQPEAAGDYLL"
     gene            8569..8658
                     /gene="yldA"
                     /locus_tag="ACHKBL_003319"
     CDS             8569..8658
                     /gene="yldA"
                     /locus_tag="ACHKBL_003319"
                     /inference="COORDINATES: protein motif:HMM:NF041476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YldA"
                     /protein_id="HFO5820150.1"
                     /translation="MSEALGITAFYIFIAAIIVAAVLYLEQRW"
     gene            8783..9211
                     /gene="uspG"
                     /locus_tag="ACHKBL_003320"
     CDS             8783..9211
                     /gene="uspG"
                     /locus_tag="ACHKBL_003320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspG"
                     /protein_id="HFO5820151.1"
                     /translation="MYKTIIMPVDVFEMELSDKAIRHAEFLAQDDGVIHLLHVLPGSA
                     SLSLHRFAADVRRFEEHLQHEAEERLQTMVSHFTIDPSRIKQHVRFGSVRDEVNELAK
                     ELDADVVVIGSRNPSISTHLLGSNASSVIRHANLPVLVVR"
     gene            complement(9332..10897)
                     /gene="ahpF"
                     /locus_tag="ACHKBL_003321"
     CDS             complement(9332..10897)
                     /gene="ahpF"
                     /locus_tag="ACHKBL_003321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008785 - alkyl hydroperoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000302 - response to reactive oxygen
                     species [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HFO5820152.1"
                     /translation="MLDTNMKTQLKAYLEKLTKPVELIATLDDSAKSAEIKELLAEIA
                     ELSDKVTFKEDNSLPVRKPSFLITNPGSNQGPRFAGSPLGHEFTSLVLALLWTGGHPS
                     KEAQSLLEQIRHIDGDFEFETYYSLSCHNCPDVVQALNLMSVLNPRIKHTAIDGGTFQ
                     NEITDRNVMGVPAVFVNGKEFGQGRMTLTEIVAKIDTGAEKRAAEELNKRDAYDVLIV
                     GSGPAGAAAAIYSARKGIRTGLMGERFGGQILDTVDIENYISVPKTEGQKLAGALKVH
                     VDEYDVDVIDSQSASKLIPAAVEGGLHQIETASGAVLKARSIIVATGAKWRNMNVPGE
                     DQYRTKGVTYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFAPEMKA
                     DQVLQDKLRSLKNVDIILNAQTTEVKGDGSKVVGLEYRDRVSGDIHNIELAGIFVQIG
                     LLPNTNWLEGAVERNRMGEIIIDAKCETNVKGVFAAGDCTTVPYKQIIIATGEGAKAS
                     LSAFDYLIRTKTA"
     gene            complement(11068..11631)
                     /gene="ahpC"
                     /locus_tag="ACHKBL_003322"
     CDS             complement(11068..11631)
                     /gene="ahpC"
                     /locus_tag="ACHKBL_003322"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HFO5820153.1"
                     /translation="MSLINTKIKPFKNQAFKNGEFIEITEKDTEGRWSVFFFYPADFT
                     FVCPTELGDVADHYEELQKLGVDVYAVSTDTHFTHKAWHSSSETIAKIKYAMIGDPTG
                     ALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVAS
                     HPGEVCPAKWKEGEATLAPSLDLVGKI"
     gene            12003..12749
                     /gene="dsbG"
                     /locus_tag="ACHKBL_003323"
     CDS             12003..12749
                     /gene="dsbG"
                     /locus_tag="ACHKBL_003323"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
                     /protein_id="HFO5820154.1"
                     /translation="MLKKILLLALLPAIAFAEELPAPVKAIEKQGITIIKTFDAPGGM
                     KGYLGKYQDMGVTIYLTPDGKHAISGYMYNEKGENLSNTLIEKEIYAPAGREMWQRME
                     QSHWLLDGKKDAPVIVYVFADPFCPYCKQFWQQARPWVDSGKVQLRTLLVGVIKPESP
                     ATAAAILASKDPAKTWQQYEASGGKLKLSVPANVSTEQMKVLSDNEKLMDDLGANVTP
                     AIYYMSKENTLQQAVGLPDQKMLNIIMGNK"
BASE COUNT         3037 a         3383 c         3252 g         3182 t
ORIGIN      
        1 gagaaaaaga tatacctcga tcgcccccct ttctcccaag tgaaaataaa aggttatcag
       61 tttgcaacat cgaacaacat ttgttgcaaa tcgataacaa catgcacctt caggatacta
      121 tttattatgt tcggcaatga tattttcacc cgtgtaaaac gttcagaaaa taaaaaaatg
      181 gcggaaatcg cccaattcct gcatgaaaat gatttgagcg ttgacaccac agtcgaagta
      241 tttattaccg taacccgcga tgaaaagctt atcgcgtgcg gtggaattgc cggaaatatt
      301 attaaatgcg ttgctatcag tgaatccgtt cgcggtgaag gactggcgct gacattagcc
      361 accgagctaa ttaacctcgc ctatgagcgg cacagcacgc atctatttat ttataccaaa
      421 accgaatacg aggcgctgtt ccgccagtgc ggtttttcca cgctgaccag cgtacccggc
      481 gtgatggtgc tgatggaaaa cagcgccacg cgactgaaac gctatgccga atcgctgaaa
      541 aaattttgtc atccagggaa caagattggc tgcattgtga tgaacgccaa tccctttacg
      601 aatggtcacc gttatctgat tcaacaggct gcggcacagt gcgactggtt gcatctgttt
      661 ttagtcaaag aagattcttc acgcttcccc tatgaagacc gactggatct ggtgttaaaa
      721 ggcaccgccg atattccacg cctgactgtg catcgcggct ccgaatacat catctcccgc
      781 gctacgttcc cttgctactt cattaaagaa cagagcgtca ttaaccattg ttacaccgaa
      841 attgatctga agattttccg tcagtacctc gctcccgcgc tgggtgtaac tcaccgcttt
      901 gtcggtactg aacccttttg tcgcgttact gcccagtaca accaggatat gcgctactgg
      961 ctggaaacac cgactatctc cgcaccgccc atcgaactgg ttgaaattga gcggctgcgt
     1021 taccaggaga tgccgatatc cgcttcccgg gtacgtcaac tgctggcgaa aaacgatctc
     1081 acggctatcg cgccgctggt ccctgcagtc acgctgcatt atttgcagaa cctgcttgag
     1141 cactcccgcc aggacgcggc agctcgtcaa aagacccccg catgagaaac aggtgaaaaa
     1201 tgaaaataaa ccagcccgcc gttgcaggca cccttgagtc tggggatgtg atgatacgca
     1261 tcgccccact cgatacgcag gatatcgacc tgcaaatcaa cagcagcgtt gagaaacagt
     1321 ttggcgatgc aattcgcacc accattctgg acgttctcgc ccgctacaac gtgcgcggcg
     1381 tacagctgaa tgtcgatgac aaaggcgcac tggactgcat tttacgtgca cgactggaag
     1441 ccctgctggc acgcgccagc ggtatcccgg ctctgccatg ggaggattgc caatgatttc
     1501 cgcttcgctg caacaacgta aaactcgcac ccgccgcagt atgttgtttg tgcctggtgc
     1561 taatgccgcg atggtcagca actcctttat ctacccggct gatgccctga tgtttgacct
     1621 cgaagactcc gtagcattgc gtgaaaaaga caccgcccgc cgcatggttt accacgcgct
     1681 gcaacatccg ctttatcgcg atattgaaac cattgtgcgt gtaaacgcgc tggattccga
     1741 atggggtgtt aacgacctgg aagctgtcgt tcgcggtggt gcggacgttg tgcgtctgcc
     1801 gaaaaccgat accgctcagg atgttctgga tatcgaaaaa gagatcctgc gtatcgaaaa
     1861 agcctgtggt cgtgaacccg gcagtaccgg cctgctggcg gcgattgaat ctccgctggg
     1921 cattacccgc gcggtagaaa tcgctcacgc ttccgagcgt ttgatcggta tcgccctcgg
     1981 tgcagaagac tatgtgcgca acctgcgtac agaacgctcc ccggaaggaa ctgaactgct
     2041 gttcgctcgc tgctccattt tgcaggccgc gcgctctgcg ggtattcagg cgttcgatac
     2101 cgtctattcc gacgctaaca acgaagccgg attcctgcaa gaagccgccc acatcaaaca
     2161 gctgggcttt gacggcaaat cgctgatcaa cccgcgtcag attgatctgc tgcacaacct
     2221 ctacgcaccg acccagaaag aagtggatca cgcccgccgc gtcgtagaag ccgctgaagc
     2281 cgccgctcgc gaaggcctcg gcgtggtttc cctgaacggc aagatggtgg acggtccggt
     2341 tatcgatcgc gcccgtctgg tgctctcccg tgcagaactt tccggcatcc gcgaagaata
     2401 aggcaatcaa aatgacgcag aaaattgaac aatctcaacg acaagaacgg gtagcggcct
     2461 ggaatcgtcg cgctgaatgc gatcttgccg ctttccagaa ctcgccaaag caaacctacc
     2521 aggctgaaaa agcgcgcgat cgcaaactgt gcgccaacct ggaagaagcg attcgtcgct
     2581 ctggtttaca ggacggcatg acggtttcct tccatcacgc tttccgtggc ggtgacctga
     2641 ccgtcaatat ggtgatggac gtcatcgcga agatgggctt taaaaacctg actctggcct
     2701 ccagctccct gagtgattgc catgcgccgc tggtagaaca cattcgccag ggcgtggtta
     2761 cccgcattta tacctctggc ctgcgcggtc cactggcgga agagatctcc cgtggtctgc
     2821 tggcggaacc ggtgcaaatc cactctcacg gcggacgtgt gcatctggta cagagcggcg
     2881 aactgaatat cgatgtggct ttcctcggcg tcccgtcctg tgatgaattc ggtaatgcca
     2941 acggctactc cggtaaagcc tgctgcggct ccctcggcta tgcaatggtt gatgccgaca
     3001 acgcaaaaca ggtcgtgatg cttaccgaag aactgctgcc ttatccgcat aatccggcaa
     3061 gcattgagca agatcaggtt gatctgatcg tcaaagttga tcgcgttggc gatgctgcaa
     3121 aaatcggcgc tggtgctacc cgtatgacca ctaacccgcg cgaactgctt attgcccgta
     3181 gcgctgcgga tgtgattgtt aactctggct acttcaaaga aggtttctcc atgcagaccg
     3241 gcaccggcgg cgcttcgctg gcggtaaccc gtttcctgga agacaaaatg cgtagccgcg
     3301 atattcgcgc cgacttcgcc ctcggcggta ttaccgcaac gatggtttat ctgcacgaaa
     3361 aaggtttgat ccgcaaactg ctggatgtgc agagctttga cagccatgct gcgcaatcgc
     3421 tggcccgtaa ccccaatcac atcgaaatca gcgccaacca gtacgctaac tgggggtcga
     3481 aaggcgcagc ggttgatcgt ctcgacgtgg tggtactgag cgcgctggaa attgacaccc
     3541 agttcaacgt taacgtgctg accggctctg acggcgtact gcgtggtgct tccggtggtc
     3601 actgcgatac cgcgattgct tctgcgcttt ccatcatcgt cgcgccgctg gtacgcggtc
     3661 gtattccgac gctggtggat aacgtgctga cctgcatcac cccgggctcc agtgtcgata
     3721 ttctggtcac agaccacggc atcgctgtta acccggcacg tccggagctg gcagaacgtc
     3781 tgcaggaagc gggcattaaa gtggtttcca ttgagtggct gcgtgaacgt gcgcgtctgc
     3841 tgaccggtga accacagccg attgaattca ccgaccgcgt cgttgccgtt gtgcgttacc
     3901 gcgatggctc ggtgatcgat gttgtgcatc aggtgaagga ataagccatg cacctgcttc
     3961 ctgaactcgc cagccaccat gcggtgtcaa ttcccgagct gctcgtcagc cgggatgaaa
     4021 ggcaagcacg gcaacacgtc tggctcaagc gccatcctgt tccactggtc tcctttaccg
     4081 tggttgcgcc tgggccgatt aaagacagcg aggtcacacg ccgaattttt aatcatggcg
     4141 tgacagcctt gcgtgcctta gccgcaaaac tgggctggca aattcaggag caggctgcac
     4201 tggtttccgc cagcgggccg gagggcatgt tgagcattgc cgccccggct cgcgacctca
     4261 agctcgccac cattgagctt gaacatagtc atcctctcgg gcggttatgg gatatcgatg
     4321 tcctgacacc cgaaggcgaa attctctccc gccgcgacta ttcactgccg cctcgccgct
     4381 gcctgttgtg cgagcaaagc gcagctgttt gcgcgcgtgg aaaaacccat caactgaccg
     4441 atttactcaa ccgcatggag gcgctgctga acgatgtcga tgcctgcaac gtcaactaaa
     4501 aacacaaagc ttgcgacgtc attaatcgat gagtacgccc tgctgggctg gcgcgccatg
     4561 ctgactgaag tcaatctgtc accgaaacca ggcctcgtgg atcgcattaa ctgcggtgcg
     4621 cacaaagata tggcgctgga agatttccac cgcagcgcgc tggcgattca gggctggtta
     4681 ccccgtttca ttgaatttgg tgcctgtagt gcagaaatgg caccggaagc ggtactcaac
     4741 ggattacgcc caattggtat ggcttgcgaa ggtgatatgt tccgcgccac tgcgggcgta
     4801 aacacgcata aaggaagcat tttttcttta gggctactgt gtgcggcaat tggtcgtttg
     4861 cttcagctca accagccggt aacgccaaca accgtttgtt ctacggcggc aagtttctgc
     4921 cgtggcctga ccgatcgcga actgcgtacc aataattcac gactgacggc aggtcaacgg
     4981 ttgtaccagc agcttggcct taccggcgca cgcggtgaag ccgaagcggg ttatccactg
     5041 gtgatcaacc acgccttgcc gcattacctc actctgctgg atcaggggtt agatcctgag
     5101 ctggcattgc tcgatacctt gctcctactg atggctacca acggcgatac caacgttgca
     5161 tcgcgcggtg gcgagggggg cctgcgctgg ctacagcgcg aggcgcaaac attattgcaa
     5221 aaagggggca ttcgaacccc cgccgatctc gattatctcc ggcagttcga cagggagtgt
     5281 atcgaacgaa atctcagtcc aggcggcagt gccgacctgc tgatccttac ctggttttta
     5341 gcacagattt aattatttaa gcacttgata aatttggaaa tattaatttt cggagaaccc
     5401 gtatgtcttt agcaaaagat aatatatgga aattattggc gcccctggtg gtgatgggtg
     5461 tcatgtttct tatccctgta cctgacggta tgccgccgca ggcgtggcat tactttgcag
     5521 tgtttgtggc aatgattgtc ggcatgatcc tcgagccaat tccggcaacg gcgatcagtt
     5581 ttattgcggt tactatttgc gttattggca gtaattacct gctctttgat gccaaagaat
     5641 tagctgaccc agcgtttaat gcgcaaaaac aggcgctgaa atggggtctg gctggttttt
     5701 ccagcaccac ggtatggctg gtatttggcg catttatttt tgcattaggg tatgaagttt
     5761 ccgggttagg ccgtcgtatt gcccttttcc tggtgaaatt catgggcaaa cgcacgctga
     5821 cgttgggtta tgcgattgtc attatcgaca ttctgctggc accgtttaca ccgtccaaca
     5881 ccgcgcgtac cgggggtacg gtttttccgg tcattaaaaa cctgccgccg ttgtttaaat
     5941 cattcccgaa cgatccgtcc gcgcgtcgta ttggcggcta tttgatgtgg atgatggtca
     6001 ttagtaccag tctgagttcg tccatgtttg tcaccggtgc ggcaccaaac gtgctgggtc
     6061 tggagttcgt cagcaaaatt gccggtatcc agattagctg gttgcagtgg ttcctctgct
     6121 tcctgccggt tggggttatc ttgcttatca ttgcgccgtg gctttcctac gtgctgtaca
     6181 aaccggaaat cacacacagt gaagaagtgg caacctgggc gggtgatgaa ctaaaaacca
     6241 tgggtgcgct gacacgcaga gagtggacgc tgattggcct tgtattgctc agcttaggtt
     6301 tgtgggtatt tggcagtgaa gtcattaatg ctactgcggt tggtctgctg gcagtttcgc
     6361 taatgctggc cctgcacgtt gtgccgtgga aagacattac ccgctataac agcgcatgga
     6421 acacgctggt caacctggca actctggttg tgatggctaa cggcctgact cgttctggtt
     6481 ttattgactg gttcgccaat accatgagta cgcacctgga aggattctca ccaaacgcaa
     6541 cggtgattgt actggttctg gtgttctact ttgcacacta cctgtttgcc agcctgtctg
     6601 cgcacaccgc aaccatgctg ccggttattc tggccgtcgg taaaggtatt ccgggcgtac
     6661 caatggaaca actgtgtatc ctgctggtgc tatctatcgg tatcatgggc tgtctgacgc
     6721 cgtatgcaac cggtcctggg gtgattattt acggctgtgg ctatgtgaaa tcaaaagatt
     6781 actggcgtct tggcgcaatc ttcggggtga tttacatctc tatgttgctg ttggttggct
     6841 ggccgattct cgccatgtgg aactaatacg ttcgccaaac gctgaataaa tcatcacgcc
     6901 cgccaggtaa acccacctgg cgggcttttt tatgattaaa tagataatat tcagtgtatg
     6961 tatgagttcc acacccatta tgaaagcatt ctggcgtaac gccgcgttgc tcgcggtttc
     7021 tctgcttccc ttctcttctg ccaacgccgt ggcgttgcag gcaaaacagt atggcgattt
     7081 tgatcgctat gtcctggccc tctcctggca aaccggattt tgccagagtc aacacgatcg
     7141 aaatcgtaac gaacgagatg aatgtcgcct gcaaaccgaa acgaccaaca aagctgattt
     7201 tctgaccgta catggtctgt ggccaggatt gcctaaatcg gttgctgccc gtggtgttga
     7261 tgaacgccgc tggatgcgct tcggttgcgc tactcgccca atcccgaatc taccagaagc
     7321 gcgcgccagc cgaatgtgtt catcgccgga aaccgggtta tcactggaaa cggccactaa
     7381 actaagtgaa gtcatgccag gagctggcgg acgttcctgc ctggaacgct acgaatatgc
     7441 caaacacggt gcctgctttg gttttgatcc agacgcatac ttcggcacga tggtacgcct
     7501 gaatcaagaa attaaagaaa gcgaagccgg aaaattcctt gcggataact acggtaaaac
     7561 agtgagccgc cgtgactttg atgccgcctt tgccaaaagc tggggaaaag agaacgtgaa
     7621 agcagttaag ctaacgtgcc agggtaaccc tgcgtatttg actgaaattc agatctcggt
     7681 caaagctgac gccatcaacg ctccgctttc tgccaactca ttcttgccac aaccacaccc
     7741 aggcaactgt ggcaaaacct ttgtgattga taaagcaggt tattaatcct ctgatttaca
     7801 aaacacgcta attcgtcttc cataaaccat gacgattaag atttatacat aatgcgctac
     7861 attgaggaat tccctgaatg tgacgcaaat cacttcaaag agtgagcaac agtcagtatc
     7921 atcatgtgag ttaaaccctc gccgcctgac ggtgagggtt ttcttttggg tatgtttatc
     7981 tgcgacactc gcagtaccga cgacatggag taaaaatgtc cagaccaact atcatcatta
     8041 acgacctgga tgccgaacgc atcgatattc tgctggagca acccgcctat gctggtttgc
     8101 caatcgccga cgcgttaaac gcagagttgg atcgcgccca aatgtgttcg ccagaagaga
     8161 tgccacacga cgtggtgaca atgaacagcc gggttaaatt ccgcaatctt agcgatggcg
     8221 aagtgcgtgt gcgcacgctg gtgtatccgg caaaaatgac cgacagcaat actcagcttt
     8281 ccgttatggc tccggtaggt gccgcactgc tggggctgcg cgttggcgat tccattcact
     8341 gggaacttcc gggcggcgtt gcaacccacc ttgaagtgct ggaactcgaa taccagccag
     8401 aagctgctgg cgactacctg ctttaatccc atctgcacaa gcgataactc gctttgccca
     8461 gaggatgcac tcgcatcctc tttccgctca aatatcaacc agatttatca ttttgctctt
     8521 aagcgcagca ttctaatctg aactggctat tcatctgcgg aacctcatat gagcgaagct
     8581 ctcggtatca ctgcatttta tatattcata gcagcaatta ttgttgctgc agtgctatac
     8641 cttgaacaac gctggtagcg cctggttttt atttattttc cagctcaata gcaaattcag
     8701 atcaaatcag cctcgcaaat tatagattcg taaccaataa tgtgctgtta tgtatacatg
     8761 caaaaacaag gagagaatgc ttatgtacaa aacaatcatc atgccagtgg atgttttcga
     8821 aatggaattg agcgacaaag cgattcgcca cgctgaattc ctcgcccagg atgacggagt
     8881 tattcatcta cttcacgtac taccggggtc agccagcctg agcctgcacc gttttgccgc
     8941 tgatgtacgt cgttttgaag agcatctgca acatgaagca gaagaacgtc tgcaaacgat
     9001 ggtcagccac ttcaccatcg atccttcccg cattaagcaa catgtccgtt ttggtagtgt
     9061 gcgggatgaa gttaacgagt tagcgaaaga acttgatgca gatgtagtag tgattggttc
     9121 gcgtaaccca tcaatttcaa ctcatctgtt gggttctaac gcctcgagcg taatccgcca
     9181 cgccaatttg ccggtgctgg ttgtgcgtta attgccagaa taagtctccc gccctgcccg
     9241 gagccatccc tgcagggctt tttaatgcaa taaaaaagcc gccaggtttg accccggcgg
     9301 ctaagcaatt acaggtgaat cttacttctt cttatgcagt tttggtgcga atcaggtagt
     9361 caaaagcact cagagaggct ttggcacctt cgccagtggc gatgatgatc tgcttgtacg
     9421 gaaccgtcgt acagtcaccc gctgcgaaca cgcctttgac gttggtttcg catttcgcat
     9481 caatgataat ctcgcccatg cgattacgtt cgactgcgcc ttccagccag ttggtgttcg
     9541 gcagcagacc aatctgcacg aaaataccgg ccagttcgat gttgtgaata tcgccgctga
     9601 cacgatctcg atattccaga cctacgactt tgctaccgtc gcctttcact tccgtggttt
     9661 gcgcattcag aataatgtcg acgtttttca ggctgcgcag tttgtcctgc agaacctggt
     9721 cggctttcat ttctggcgca aattccagca gcgttacgtg ctcaacgata cccgccaggt
     9781 caattgccgc ttccacgccg gagttaccgc cgccgataac cgctacgcgt ttacctttaa
     9841 acagcgggcc gtcgcagtgc gggcagtagg tcacgccttt ggtgcgatac tgatcttcgc
     9901 ccggaacgtt catgttgcgc cattttgcac cggtcgccac gataatgctg cgtgctttca
     9961 gtaccgcgcc agaagctgtt tcaatctgat gcaggccacc ttcaaccgct gccgggatca
    10021 gtttgctggc gctctggctg tcgatcacat caacgtcgta ttcatcaacg tgaactttca
    10081 gcgctcctgc cagtttctgg ccttcggtct tcggtacaga aatgtagttt tcgatatcaa
    10141 cggtatcgag gatctgacca ccaaaacgtt cgcccatcag accggtacgg atgcctttac
    10201 gtgcggagta aattgctgcc gctgcacccg ccgggccgga accgacgatt aatacgtcat
    10261 aagcatcacg cttgttcagc tcttctgccg cacgtttttc cgcaccagta tcaattttgg
    10321 caacgatttc agtcaacgtc atgcggccct gaccaaactc tttcccgttt acgaacactg
    10381 ccggaacgcc catcacgttg cgatcggtga tttcgttctg gaaggtgccg ccgtcaattg
    10441 cagtgtgctt gatgcgcggg ttcagtacgc tcatcaggtt cagcgcctgc accacgtccg
    10501 ggcagttgtg gcaagagagc gagtaatagg tttcgaattc aaaatcaccg tcaatatggc
    10561 gaatctgctc cagcagagac tgcgcttctt tcgacggatg accaccggtc cacagcaacg
    10621 ccagtaccag cgaggtgaac tcgtggccca gcggggagcc tgcaaaacgt ggcccctggt
    10681 tagaacctgg gttggtgatc aggaaagacg gcttacgcac cggcaagctg ttatcttctt
    10741 taaaggtgac tttgtctgac agttctgcga tttcagccaa cagttccttg atttctgccg
    10801 atttagcgct gtcatccagc gtggcaatta actcaacagg cttggtcaat ttctcaaggt
    10861 aagccttgag ttgagttttc atatttgtgt cgagcatgtt tatctcctgg gctttaaaca
    10921 tcatcatgca agcggcctct gcggctacct ggatgcagct tgcatcatgg tgcggggaag
    10981 aaaatcgggt gccgcagcac ccgaagaatt aggtgaattt ccgaagtatt tcgcgccgga
    11041 gttagtagcg cgaaagacaa aggaaattta gattttacca accaggtcca gagacggagc
    11101 cagagttgct tcaccttctt tccatttagc cgggcaaact tcacctgggt gagaagctac
    11161 gtactgtgct gctttgattt tacgcagcag gtcagacgcg tcacggccaa tgccttcagc
    11221 ggtaacttcg attgcctgga tgataccctg cgggtcaaca acgaaggtcg cacggtcagc
    11281 cagaccttca tcttcacgca tgttgtcgaa gttacgggtc agggcgccag tcgggtcgcc
    11341 gatcatcgca tatttgattt tagcgatggt ttcagagctg ctgtgccatg ctttgtgggt
    11401 gaagtgagta tcggtagata ctgcgtatac gtctacgccc agtttctgca attcttcgta
    11461 atggtcagca acgtcaccca gttcggtcgg gcatacgaaa gtaaagtcag ccgggtagaa
    11521 gaagaagacg ctccagcggc cttcggtatc tttttcggtg atttcgatga attcgccgtt
    11581 tttgaatgcc tggtttttaa aaggtttaat tttggtgtta atcaaggaca tctatacttc
    11641 ctccgtgttt tcgatgagat gtaaggtaac caatttttgc cgattcggct aatgcgtttc
    11701 cattatcaaa tcgataagtt ttaccttaca accttcgtaa gacaactttg tgaactttgc
    11761 accgccagcg gcgataaaaa gtaaaatggc cccctttcca ggtgaccgtg taatctgaac
    11821 taccctttgg tatcttcagc gcctgcctgc aggctgaggt gttgccctta caaatgcaac
    11881 aacgacatgg attacaacac cctcataaac aaagggcaat cacctgatct aagctcttac
    11941 ctatgacagt gataggttat gccttttact cgacttttgc actgactgaa aaggacaaat
    12001 taatgttaaa aaagatactt ttactggctc tgcttcctgc aatcgccttc gcagaggaac
    12061 ttcctgcccc agtaaaagcg attgaaaaac agggtattac aatcatcaaa acgttcgatg
    12121 cccctggagg aatgaaaggt tatctcggta agtatcagga tatgggcgtc accatctacc
    12181 tgactccaga tggtaagcac gctatctctg gttacatgta caacgaaaaa ggtgaaaacc
    12241 tgagtaacac gcttatcgaa aaagaaattt acgcacccgc cggacgtgaa atgtggcaac
    12301 ggatggaaca atcccactgg ctcctcgacg gtaaaaaaga tgcgccggtc attgtctacg
    12361 tcttcgccga tccgttctgc ccatattgta aacaattctg gcagcaggct cgtccgtggg
    12421 tagattctgg caaagtgcaa ttaagaacat tgttggttgg ggttatcaag ccagaaagcc
    12481 cggcgacagc agcggcaatt cttgcctcca aagatcccgc aaaaacctgg caacaatatg
    12541 aagcctctgg tggcaagctt aagctaagcg tgcctgcaaa cgtaagcaca gagcaaatga
    12601 aagtgttaag cgacaacgag aaactcatgg acgatctggg ggcaaatgtc acaccagcta
    12661 tctattacat gagtaaggaa aataccctgc aacaagccgt cggattaccc gatcagaaaa
    12721 tgcttaatat cattatgggg aataaataaa agataattca tggatggttg aaatatatcc
    12781 ttgcttttca accatcctaa aaacgcatga attgctcgta acccacctga cagctatgct
    12841 attttagaaa atct
//
LOCUS       DAZFLD010000135        12811 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.136, whole genome shotgun sequence.
ACCESSION   DAZFLD010000135 DAZFLD010000000
VERSION     DAZFLD010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12811
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.136"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..236)
                     /locus_tag="ACHKBL_003324"
     CDS             complement(<1..236)
                     /locus_tag="ACHKBL_003324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFO5820155.1"
                     /translation="MQDIHEESLNESVKSEQSPRVVLWEIDLTVQGGERYFFCNELNE
                     KGEPVTWQGREYQAYPIEGSGFEMNGKGSSARPSL"
     gene            complement(236..565)
                     /locus_tag="ACHKBL_003325"
     CDS             complement(236..565)
                     /locus_tag="ACHKBL_003325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO5820156.1"
                     /translation="MKTFRWKVKPDMEVNSQPSVREVRFGDGYSQRMAAGLNADLKTY
                     RVTLSVTREEARHLEAFLAEHGGWKAFLWKPPYAYRQIKVTCAGWSARVGMLRVEFSA
                     EFKQVVN"
     gene            complement(562..3207)
                     /locus_tag="ACHKBL_003326"
     CDS             complement(562..3207)
                     /locus_tag="ACHKBL_003326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO5820157.1"
                     /translation="MAGNFADLTAVLTLDSARFSEEAARVKKELGETSALADLMSGKV
                     SQSFRKQADAAEQSLSRQALAAQKAGISVGQYKAAMRTLPAQFTDIVTQLAGGQNPFL
                     IMLQQGGQISDSFGGPLSLLTLLKEELLGIRDASESSEDSLSDTANALAENARNAGEL
                     GRFMSVARVAAGGGVAVLAALAAAAWQAEQADRALLRSLTLTGGAAATTTAELWKMAG
                     VISDEAGGGIRQAAENLARLAESGKYTAGQLRIMGETSQRWLQTVGDDAGKVEKAFEG
                     IAADPVKALASLNQQYNFLSVSQLRHIDELERTKGKQAAVTEAMSLFADVMNARLEQL
                     DKAATPVEKIWDDVKTWTSDAWAWIGDHTLGALSLITDVVAGTVEQVKLLLVQGDLAL
                     AEFIQSAWETTKNVPGVGALFGELAEENRVFIEKTKRDELALRKSIAERDVRIRQGEM
                     GYINRSRATGVSKGPGQQEAVSRLAEELTGKKHTSPKTRSAGEREEEQAREALLALEA
                     ELRTLEKHSGANEKISRQRRDLWKAESQYAVLKEAATKRQLSGQEKSLLAHKDETLEY
                     KRQLAELGDKVEYQKRLNELAQQAVRFEEQQSAKQAAISAKARGLTDRQAQRESEEQR
                     LRDVYGDNPQALAQVTGALKQTWADEDMLRGDWLAGLKSGWGEWAESATDSFSQVKSA
                     ATQTFDGIAQNMAAMLTGAEADWRGFTRSVLSMMTEILLKQAMVGIVGRIGSAIGGAF
                     GGGASASSGTAIEAAAANFHFATGGFTGTGGKYEPAGIVHRGEFVFTKEATSRIGVGN
                     LYRLMRGYAEGGYVGGAGSPAQMRRAEGINFNQNNHVVIQNDGINGQAGPQLMKAVYD
                     MARKGAQDELRLQLRDGGMLSGSGR"
     gene            complement(3257..3559)
                     /locus_tag="ACHKBL_003327"
                     /pseudo
     CDS             complement(3257..3559)
                     /locus_tag="ACHKBL_003327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309140.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
     gene            complement(3586..4008)
                     /locus_tag="ACHKBL_003328"
     CDS             complement(3586..4008)
                     /locus_tag="ACHKBL_003328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HFO5820158.1"
                     /translation="MFLKTEQFEYNGVSVTLSELSALQRFDYMKFVSDAAQQETTEHD
                     AVHINQRYLETASLLVAMSLWHSHSLKGTLASPETEMQQIRREVMLGWPADALNQATN
                     RVLYLSGMLDNRHDADPEPTGKAEATEPVTSKKHSKAS"
     gene            complement(4025..4774)
                     /locus_tag="ACHKBL_003329"
     CDS             complement(4025..4774)
                     /locus_tag="ACHKBL_003329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO5820159.1"
                     /translation="MTLPNPLAPVKGSGTTLWVYTGNGDAWANPLSDDDWTRLAKIKD
                     LTPGEMTAESYDDNYLDDEDADWVSTGQGQKSAGDTSFTLAWKPGEKGQRDLIAWFDS
                     SETRAYKIRFPNGTVDVFRGWVSAIGKSVTAKEVITRTVKITNIGRPSLAEDRGEITP
                     VTGITVTPLTGNVAKGQNITLTVAVQPEGATDKTFRATSANQNFATITVKGNTIIVKG
                     VAAGKAQIPVVTGNGEFAAVAEITVTDGAAG"
     gene            complement(4782..5180)
                     /gene="gpU"
                     /locus_tag="ACHKBL_003330"
     CDS             complement(4782..5180)
                     /gene="gpU"
                     /locus_tag="ACHKBL_003330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail terminator protein"
                     /protein_id="HFO5820160.1"
                     /translation="MKHREIRAAVLSALKENISERVNWFDGRPVFIDEQELPAVAVYL
                     TDASATEEFVDEGTWEAVLHIEVFLRAKETDSVLDMWMEEKILPALEAVPGLSALLLK
                     MNLQGYDYRRDDEYMMWGSADLQWNITYQM"
     gene            complement(5193..5816)
                     /locus_tag="ACHKBL_003331"
     CDS             complement(5193..5816)
                     /locus_tag="ACHKBL_003331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO5820161.1"
                     /translation="MAIKGLAQAMKNLDAIDRRAVPRASATTLNRVAGAIIAKTASSV
                     ARELAVPRRLIRARIRLSPARPDKVYAKVYINTGNLPAIKLGEARVRLSRRKRRKKGQ
                     RAALKGGGSVLIVGKRRIPDAFITRLANGRWHVMQRMPWAPSSSGADSKGRPKRHRLP
                     IEVVKIPTAGPLAETFERERDRMYREKLPAQMMTAMAHQLRLVLKRK"
     gene            complement(5819..6100)
                     /locus_tag="ACHKBL_003332"
     CDS             complement(5819..6100)
                     /locus_tag="ACHKBL_003332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA breaking-rejoining protein"
                     /protein_id="HFO5820162.1"
                     /translation="MSDLFTRMCCRMDGATVRVMGKQAEINGVVYDVMPEEESAEMGA
                     LSGSQLSLVVFSARYRPARHDVVVFAGRTLTVTRYDTYNGKPRIFVEQE"
     gene            complement(6093..6419)
                     /locus_tag="ACHKBL_003333"
     CDS             complement(6093..6419)
                     /locus_tag="ACHKBL_003333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsid cement protein"
                     /protein_id="HFO5820163.1"
                     /translation="MAKNFVQDGTTIELVNAGDQTILSGAAVVVGSMVAVAITDIPAG
                     DAGDGFAEGVFLLPKQSADDIQSGAVVYLKDGVVQLAADGAVAAGVAWENAPANSATV
                     AVKINV"
     gene            complement(6507..8486)
                     /locus_tag="ACHKBL_003334"
     CDS             complement(6507..8486)
                     /locus_tag="ACHKBL_003334"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001369631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ClpP-like prohead protease/major capsid protein
                     fusion protein"
                     /protein_id="HFO5820164.1"
                     /translation="MKNKSWFRMQAGGPGDADIYIYDEIGFWGVTAKQFVSELNALGD
                     ITHINLHINSPGGDVFEGIAIFNALKNQGATITVYVDGVAASMASVIAMAGDTVIMPE
                     NAFMMIHKPWGFSGGDAEDMRSYADLLDKVESVLLPAYAQKTGKTTDEIAAMLADETW
                     MSGAECLAHGFADQVTPAVEAMACIQSKRTEEFKKMPESIRNMITPPRNSAPRDTTVT
                     IPAPAVTEPSPVPAVSDEATIRARVMAEQKARMSGINDLFAMFGGRYQTLQAQCVADP
                     DCSLEMARERLLNEMGKESSPTNKNTPAHIYAGNGNFVGDGIRQAMLARAGFENVEKD
                     NAYNGMTLREWARMSLTERGIGVASYNPMQMVGLALTHSTSDFGNILLDVSNKGLIQG
                     WEESEETFQKWTRKGRLSDFKTAYRVGMGGFGSLRQVREGAEYKYITTSDRKETIALA
                     TYGEIFSITRQAIINDDLNMLVDVPMKMGRAAKATIGDLVYKVLTDNPKLSDGKALFH
                     ADHKNIATGGISVSGLDAARQMMRLQKEGERALNIRPAFMLVPVALETVANQTIKSAS
                     VKGADANAGVINPIQNFAEVIAEARLDAADPKTWYLAAAQGTDTIEVAWLDGVDTPYI
                     DQQEGFTTDGIATKIRIDAGVAPLDWRGLVRSSVA"
     gene            complement(8476..9978)
                     /locus_tag="ACHKBL_003335"
     CDS             complement(8476..9978)
                     /locus_tag="ACHKBL_003335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO5820165.1"
                     /translation="MAIIDDVIGVFSPGWKAARLRSRALIMAYEAVKPTRTHKARREN
                     RSADQLSKYGAVSLREQARFLDINHDLVIGVFDKLEERVIGARGIIVEPQPLRKNGEM
                     VAELAADIRRLWAEWSVSPDVTGQYTRPVLERLLLRTWLRDGEVFAQMVSGAGNGLER
                     TAGVPFWLEAMEPDFVPMRTDESAGLNQGVFLDEWGRPKKYLVYKNYPVSGRQSDTKE
                     IAAGKMIHLKFTRRLHQTRGSSMLSGVLMRISALKEYEDAELTAARIAAALGLYIRKG
                     DGQDYEDPGIKETEREVHITPGIIYDDLRKGEDIGMVKSDRPNPNLETFRNGQLRAVA
                     AGSRLSFSSAARNYNGTYSAQRQELVESTDGYLILQDCFIGAVTRPVYRTWLNMVVAA
                     GLLKIPADVEMKTLYNATYSGPVMPWIDPVKEAEAWRIQIRGGAATESDWVRAGGRNP
                     DEVKRRRKAEIDENSRLGLVFDTDPVNDKGGNSAGTERQYQRDTESQHEE"
     gene            complement(9978..10190)
                     /locus_tag="ACHKBL_003336"
     CDS             complement(9978..10190)
                     /locus_tag="ACHKBL_003336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5820166.1"
                     /translation="MSTEEAREMIQRYREAEMAVLEGKSVTFNGQQLTLESLSQIRAG
                     RQEWERRLAAMVSRRRGKPGFKLARF"
     gene            complement(10187..12310)
                     /locus_tag="ACHKBL_003337"
     CDS             complement(10187..12310)
                     /locus_tag="ACHKBL_003337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HFO5820167.1"
                     /translation="MNQVNESHSRASDIWREVASLFRPPGRLPVAEAIRRYMRVPRGA
                     NTSGPWESSLTPYMIDPINTLSAREYDAVVFVGPARTGKTEGLIDGWIVYGIICDPAD
                     MLVVQMTETKAREHSRTRLSRTFRHSPEVCKRLSPSRNDNNVHDKMFLDGSFLKIGWP
                     SITVFSSSDYRRVALTDYDRFPENVDGEGDAFTLASKRTTTFMSSGMTLVESSPGRDI
                     TDTKWRCGGAHEAPPTTGILSLYNRGDRRRWYWPCPHCGEYFQPVMDNMTGYRNNPDF
                     VAAGQAARLMCPHCRGLIAPEQKRELNNQGIWLREGERAAADGSITGTPRNSRIASFW
                     MEGPAAAFQTWEQLIFKLLAAEEEYERTGSEETLKAVVNTDIGRPYLPRSATEQRKSE
                     LLEQRAEPFPRRSVPDGVRFIEATVDVQGGKNRRFVVQITGYGEQGERWIVDRYNIRH
                     SLRCSPNGESLPVDPAAYPEDWDLLLTDVFHKTWPLASDPDVRMRLMAMAVDTGGEAG
                     VTDNAYRFWRRCRSDGLGNRVFLFKGDGLRRDRLINRTFPDNTGRSARRARASGDVAL
                     WLVQTDAFKDRVNNALWRDTPGPNYIHFPDWLGRWFYDELTYEERGSDGKWRKPGRGA
                     NEAFDLLVYADALAVLHGYEKIRWPSAPDWAQRETWLVFPQARSGETVSPELTAGAEK
                     RRRRKKKLRTERAEDNPWITSGGWL"
     gene            complement(12307..12783)
                     /locus_tag="ACHKBL_003338"
     CDS             complement(12307..12783)
                     /locus_tag="ACHKBL_003338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1441 family protein"
                     /protein_id="HFO5820168.1"
                     /translation="MDRELKNLHLNISQLAALSGAHRQTVAARVKNISPAGGHESNLK
                     LYRLTDILAELMKAPLPVDNEEMDPHARKAWYQSERDRLKFEQETGLLVPVSDVRRSF
                     SVVVKAIVQVLETRPDRLERDRGWTASQLNEVQIVVDEIRDTLEKAVIDCCDEADM"
BASE COUNT         2647 a         4131 c         3051 g         2982 t
ORIGIN      
        1 agcgacgggc gggcactgct gccctttccg ttcatctcaa agccgctgcc ctcaatcggg
       61 tacgcctgat attcacgccc ctgccaggtc accggctccc ctttttcatt cagctcattg
      121 cagaaaaaat accgctcacc gccctgcacc gtcaggtcga tttcccagag caccacccgc
      181 ggtgactgct ctgacttaac cgactcgttc aggctttctt cgtgaatatc ctgcatcagt
      241 tcaccacctg cttaaactcc gcgctgaact caacgcgcaa catcccgacc cgcgcagacc
      301 acccggcgca ggtcaccttt atctgccggt atgcataggg tggcttccac aaaaatgcct
      361 tccagccccc gtgctctgcc agaaacgctt ccagatgccg ggcctcctcc cgggtcacgg
      421 aaagcgtcac ccggtatgtt ttcaggtcag cattcagccc tgccgccata cgctgtgagt
      481 acccgtcacc aaaacgcact tcacgcaccg acggctgcga gttcacctcc atatccggct
      541 tcactttcca gcgaaaggtt ttcatcgccc gctccctgat aacataccgc catcacgcaa
      601 ctgcagccgg agttcatcct gcgccccctt acgggccatg tcatacaccg ccttcatcag
      661 ctgcggcccc gcctgtccgt ttataccgtc gttctgaatc accacgtgat tgttctgatt
      721 aaaattaata ccttccgccc gccgcatctg cgccggactt ccggcaccac ccacataacc
      781 accttccgca tagccgcgca tcagacggta aagattcccc acacctatcc ggctggttgc
      841 ctctttcgtg aaaacaaact ccccgcggtg aactatcccc gcaggctcat atttgccgcc
      901 cgtccccgta aatcctccgg tcgcgaaatg gaagttcgcc gccgcagcct caatggccgt
      961 ccccgaggaa gcagatgcac caccaccgaa agcaccacca atggcgctgc cgatacgccc
     1021 gacaattccc accatggcct gtttaagcag gatttctgtc atcatggaca gcaccgaacg
     1081 ggtgaatccc cgccagtctg cctctgcacc ggtcagcatc gccgccatat tctgtgcaat
     1141 accatcaaag gtctgcgtgg ccgcactttt aacctgcgaa aaactgtccg tcgcactttc
     1201 cgcccactcc ccccagccgg acttcagccc ggccagccag tcaccgcgca gcatgtcttc
     1261 atccgcccat gtctgtttca gtgccccggt gacctgtgcc agcgcctgcg gattatcgcc
     1321 gtacacgtca cgaagacgct gctcttcaga ctcccgctgt gcctgacggt cggtgagacc
     1381 acgggctttt gcgctgatgg cggcctgctt cgcgctctgc tgctcttcaa accgcaccgc
     1441 ctgctgtgcc agctcattca ggcgtttctg gtattcaacc ttgtcgccca gctcagccag
     1501 ctggcgtttg tactccagcg tctcgtcttt atgcgccagc agggattttt cctgcccgga
     1561 taactgtcgt ttcgtggcag cctctttcag gaccgcatac tgactttccg ccttccataa
     1621 atcacggcgc tgccgactga ttttctcatt cgcaccgctg tgtttttcca gcgtcctgag
     1681 ctcagcttca agggcaagca gagcctctct tgcctgctcc tcttccctct ccccggcaga
     1741 gcgcgttttc ggtgatgtat gctttttacc tgtcagctct tcagccagac ggctgacggc
     1801 ttcctgctgc cccggacctt tgctgacgcc tgttgcacgc gaacggttga tgtaccccat
     1861 ttccccctgg cgtatacgca catcccgttc cgcaatggat tttctcagcg ccagttcatc
     1921 gcgttttgtt ttctcaataa atacgcggtt ctcttctgcc agttcaccaa acaacgcacc
     1981 aacgccgggc acattctttg tcgtttccca ggctgactga ataaattcag ccagcgccag
     2041 atccccctgc acaagcagca gcttcacctg ttcaacggtt cctgccacca cgtcagtgat
     2101 cagactgagt gcccccagtg tatgatcacc tatccatgcc catgcgtcag aagtccaggt
     2161 tttaacatcg tcccagattt tttccaccgg cgtggccgct ttatcaagtt gctccagacg
     2221 tgcattcatg acatccgcaa acagggacat cgcctccgtc accgcagcct gtttaccttt
     2281 cgtgcgctca agctcatcaa tatggcgtaa ctgggaaacg ctcaggaagt tatactgctg
     2341 attcagggag gccagcgcct tcaccggatc tgctgcaatc ccttcaaagg ctttttccac
     2401 cttcccggca tcgtccccca ccgtctgcag ccatctctga gaggtttccc ccatgatccg
     2461 tagctgcccg gcggtatatt tcccgctttc tgccagacgg gccagatttt ctgccgcctg
     2521 tctgatacca ccaccggctt catcgctgat caccccggcc attttccaca attctgccgt
     2581 tgtggtggca gccgcccctc cggtcagggt cagtgaacgc aataaggccc ggtcagcctg
     2641 ctctgcctgc caggcggcag cagcaagcgc ggccagtacg gcaaccccgc cacctgccgc
     2701 cacacgggcc accgacataa atcgtcccag ttcaccggca ttccgggcat tttcagccag
     2761 tgcatttgcc gtatctgaca gcgaatcctc tgatgattca gaggcatccc tgatcccgag
     2821 aagttcctcc ttcagcaggg taagcaggct gagcggtcca ccgaatgaat cgctgatctg
     2881 ccccccctgc tgcagcatga taaggaaggg attctgacca ccggcaagct gagtgacaat
     2941 atccgtgaac tgtgcgggca gtgtgcgcat ggcagcctta tactgtccga ctgatatccc
     3001 ggctttttgt gcagccagcg cctgtcggct cagactctgc tcagcagcat cagcctgttt
     3061 tctgaaagac tgactgactt tcccggacat caaatcagca agcgcactgg tttcacccag
     3121 ctcttttttt acccgcgctg cctcttcaga aaaacgggca gaatccagtg tcagcacggc
     3181 tgtcagatcg gcaaaattac ctgccatcgt ggtcacctcc tgaaatgtcc tctgatgcca
     3241 tcaataactg tcacaacctc cttccctcct ccccgaaacg gactccaccg gcgaggcccg
     3301 ccgccttctg catcagtaca tcattttcgt ccggcgtctc cgcctgccct tcctctgcct
     3361 ccggagcgaa caggctgaaa tccgccggat gcatatccgg atcgcaaaaa aacaggctga
     3421 gtacggcgta cgtcagcccg gaaaaatgca tatccagctg ggtatcctga aaataatgcg
     3481 tgcagtaaaa acgtcgccag tcggcatatt cggtggatgt catcccggca agcatggcgc
     3541 gccagtcggc tctccccatc tcacgcgcca gtttcaggac aaagttcagc tcgccttcga
     3601 atgctttttt gatgttaccg gctcagtcgc ttctgctttc ccggttggtt caggatcggc
     3661 atcgtgccgg ttatccagca tacctgaaag ataaagcacc cggttcgttg cctgattcag
     3721 tgcatcagca ggccatccca gcatcacttc acggcggatc tgctgcatct ctgtctccgg
     3781 agatgccaga gtgcctttga gggaatggga atgccatagc gacatcgcca caagcaggga
     3841 tgccgtttcc agatatcgct ggttaatgtg cacggcatca tgctccgttg tctcctgttg
     3901 tgctgcgtct gaaacaaact tcatataatc aaaccgctgc agcgcagaca gctccgacag
     3961 cgtgacggac accccgttat attcaaattg ttctgttttc aggaacatgc ttgatctcct
     4021 cccctcagcc cgcagcgcca tccgtgacgg tgatctccgc caccgccgca aactcaccgt
     4081 tgccggtgac aacagggatc tgcgctttac ctgccgcaac acctttcacg atgatcgtgt
     4141 tccctttcac ggtaatggtg gcgaaattct gattcgctga ggttgcgcgg aacgttttat
     4201 ccgttgcgcc ttccggctga acggccacgg tcagggtgat attctgacct tttgccacat
     4261 tccccgttag tggcgtcacg gtaataccgg tgaccggtgt gatttctccc cgatcctcag
     4321 ccagcgacgg acgaccgata ttggtgattt tcacagtgcg ggtgatcact tctttggcgg
     4381 tcacggattt accaatggcg ctcacccagc cacggaacac atccaccgtg ccgttcggga
     4441 agcggatttt gtaggcccgg gtctcgctgc tgtcaaacca ggcaatcaaa tcacgctgcc
     4501 ctttctcgcc gggcttccag gccagcgtaa aactggtgtc accggcagat ttctgcccct
     4561 gcccggtgga tacccagtca gcatcctcat catccagata gttatcgtcg taggattccg
     4621 ccgtcatctc gccgggggtc agatccttta tttttgccag gcgcgtccag tcatcatctg
     4681 acagcgggtt tgcccaggca tcgccatttc cggtataaac ccagagcgtg gtgccggaac
     4741 cttttaccgg cgcaagggga ttagggagcg tcataacgtc ctcacatctg gtaagtaata
     4801 ttccactgga gatcagccga tccccacatc atatattcgt catcccggcg atagtcatac
     4861 ccctgaagat tcatcttcag cagtaatgca cttagcccgg gaaccgcctc cagcgccgga
     4921 agtatttttt cttccatcca catatccagc acggagtccg tctctttcgc cctgagaaac
     4981 acctcaatat gaagcacggc ctcccaggtc ccctcgtcaa caaactcctc cgtcgcagaa
     5041 gcatctgtca gataaaccgc caccgcaggc agttcctgtt catcaataaa taccgggcgg
     5101 ccgtcaaacc agttcacccg ctcagaaata ttttctttca gggcagacag aactgccgcc
     5161 cgtatttcac ggtgtttcat acaccctccc tgtcattttc gtttcagcac caggcgtaac
     5221 tgatgcgcca tggccgtcat catctgcgcc ggtaattttt cccggtacat ccggtcccgt
     5281 tcacgttcaa aggtttctgc cagcggtccg gcagtcggaa tcttcaccac ttcgatcggc
     5341 agacggtggc gtttcggcct ccctttgctg tccgcgccgc tggacgatgg tgcccacggc
     5401 atacgctgca tcacatgcca gcgtccgtta gccagccggg tgataaaggc gtccgggatc
     5461 cgtcttttcc ccacaatcag cacactgccg ccccctttca gggccgcacg ctgtcctttc
     5521 tttctccgtt ttctgcggga aagtcgaacg cgggcctccc ccagtttgat ggcgggcagg
     5581 ttgccggtat tgatgtaaac ctttgcgtaa accttatccg gtcgtgccgg acttaaccgg
     5641 atgcgggcac ggataagacg acggggaacg gccagctccc tggcaactga agaggccgtt
     5701 ttcgcaataa tggcccccgc cacgcggttc agtgtcgtgg cagaggcccg gggaacggca
     5761 cggcggtcaa ttgcatccag atttttcatg gcctgcgcca gaccttttat tgccatactc
     5821 attcctgttc gacaaaaatc cggggtttac cgttgtacgt gtcataacgg gtcaccgtca
     5881 gtgtgcgccc cgcaaacaca acaacatcat gacgggccgg acggtaccgg gctgaaaaca
     5941 ccaccagtga caactggctg cccgaaagcg cccccatctc cgcggactct tcctccggca
     6001 tcacgtcata cactacgccg ttaatctccg cctgtttgcc catcacccga acggtcgccc
     6061 cgtccatccg gcaacacatt cgcgtaaaca gatcagacat tgatttttac cgccacagtg
     6121 gcgctgtttg caggagcatt ttcccaggct acccccgcag ccaccgcacc gtctgcagcc
     6181 agctgcacaa ccccgtcctt cagataaacc accgcgccgg actgaatatc gtcagcagac
     6241 tgtttgggca gcaggaacac gccttcggca aaaccgtcac cggcatcacc ggcaggaata
     6301 tcggtaatgg ccactgccac catactgccg accaccaccg cagcaccgct caggatggtc
     6361 tgatctccgg cattcaccag ttcaatggtg gtgccgtcct gtacaaaatt tttcgccata
     6421 atgctgtttc tccggacagc ccctgtgggg ctgtttttca ggcataaaaa aagccctttc
     6481 gggcagtgat tgtgataacg cggttatcag gccaccgacg aacgcaccag accgcgccag
     6541 tcaagtggtg ccacaccggc atcaatacga atttttgtgg caatgccgtc agtggtgaaa
     6601 ccttcctgct gatcaatgta tggcgtgtcc acaccatcca gccaggccac ttcaatggtg
     6661 tcagtgccct gtgccgccgc cagataccag gttttcggat ctgccgcatc aagacgcgct
     6721 tccgcaatca cctcagcaaa gttctggatg gggttaatga caccggcgtt tgcatccgcc
     6781 cctttcacac tggccgattt gatggtctgg ttcgccaccg tctccagtgc caccggtacc
     6841 agcataaagg ccggacggat attcagggcg cgctcgcctt ctttctgcag gcgcatcatc
     6901 tgacgggccg catccagtcc ggaaacggag atcccgccgg tggcaatatt tttgtgatcg
     6961 gcatggaaca gcgccttacc gtcggacagt ttcgggttat ccgtcagcac cttgtaaacc
     7021 aggtcaccaa tcgttgcctt cgccgcacgc cccatcttca tcggcacgtc caccagcata
     7081 ttcagatcat cattgataat ggcctggcgg gtgatggaga aaatctcccc gtaggtggcc
     7141 agagcaatgg tctccttgcg atctgaggtg gtgatgtatt tatactccgc cccctcacga
     7201 acctgacgca aagaaccaaa accgcccatc cccacgcgat acgctgtttt gaagtctgac
     7261 aggcgtccct tacgggtcca cttctggaag gtttcttctg attcctccca gccctggatc
     7321 agccccttgt tcgacacatc cagcaggata ttgccaaaat cagaggtgct gtgcgtaagc
     7381 gccagcccga ccatctgcat ggggttataa ctggccaccc caataccgcg ctccgtcagt
     7441 gacatacgag cccattcacg cagggtcatc ccgttatagg cgttatcctt ctcgacattt
     7501 tcaaatccgg cacgggccag catcgcctgg cggatcccgt ctcccacaaa attgccgttt
     7561 ccggcataaa tatgggccgg tgtgtttttg ttggtcggcg aggactcctt gcccatttca
     7621 ttcagcagac gttcacgggc catttccagc gaacagtcag gatcagccac gcactgtgcc
     7681 tgaagcgtct gatagcgacc gccgaacatg gcaaacagat cgttaatgcc tgacatgcgt
     7741 gctttctgct cagccataac gcgggcgcga atggtcgcct catcagacac tgccggtacc
     7801 ggtgatggtt ctgttaccgc cggtgcaggg attgtcactg tggtatcacg cggggcactg
     7861 ttgcgtggtg gagtaatcat gtttcggatg gattccggca tctttttaaa ttcctctgta
     7921 cgttttgact gaatacatgc cattgcctca acagcgggtg tcacctggtc agcaaatccg
     7981 tgtgccagac attcggcacc ggacatccag gtttcatccg ccagcatggc ggcaatttca
     8041 tcggtggttt ttccggtttt ctgcgcatag gctggcaaca gtaccgattc gactttatcc
     8101 agcaaatcag cataactgcg catatcctca gcatccccgc cactgaatcc ccatggctta
     8161 tggatcatca tgaaggcatt ttccggcata atgaccgtat caccggccat cgcaatcaca
     8221 gatgccatcg aggcggcaac gccatccaca tacacggtaa tggtcgcccc ctgatttttc
     8281 agggcattaa aaatggcgat gccttcaaag acatcgccac ccggtgagtt gatgtggaga
     8341 ttaatgtggg tgatatcacc cagagcattc agttcgctga caaactgctt cgcggtaact
     8401 ccccagaaac caatctcgtc ataaatataa atatccgcgt cacccggccc cccagcctgc
     8461 atcctgaacc aggatttatt cttcatgctg gctttcggtg tcacgctgat actgtcgttc
     8521 agttccggca ctgttgcctc ctttgtcgtt gacggggtca gtatcaaaga ccagccccag
     8581 tctgctgttt tcatcaattt cagccttgcg gcgacgtttg acctcatccg gattgcgccc
     8641 gccggcacgc acccagtcag attctgtcgc tgcaccaccc cggatctgaa ttctccaggc
     8701 ttcagcttcc ttaaccgggt cgatccacgg catcaccgga ccggaatacg tcgcgttata
     8761 tagcgttttc atctccacat ccgccggaat tttcagcaga cctgccgcaa ccaccatatt
     8821 cagccatgtc cggtacaccg ggcgggttac cgcgccaata aaacagtcct gcaggatcag
     8881 gtaaccatcc gtggactcga ccagctcctg ccgctgggcg ctgtaggtgc cgttatagtt
     8941 acgcgccgca ctggaaaaac tcagacgact gccagctgcc actgcacgca actggccgtt
     9001 gcggaaagtt tcaaggttgg gattgggacg gtcagatttg accatgccga tatcctcgcc
     9061 cttgcgcaaa tcgtcataaa taatacccgg ggtgatatgg acttcccgct cggtttcttt
     9121 gatccccgga tcttcatagt cctgtccgtc acctttacgg atatacagtc ccagcgccgc
     9181 agcaatacgc gccgctgtca gttccgcatc ctcatactct ttaagggcac tgatccgcat
     9241 cagcaccccc gataacatgg atgagcctcg cgtctgatgc agacgacgag tgaacttcag
     9301 gtggatcatt tttccggcag cgatttcttt cgtatcactc tgccggccgc tgaccggata
     9361 atttttataa accagatatt ttttcggtct tccccactca tcaagaaaaa ccccctgatt
     9421 cagcccggcg gattcatcag tgcgcatggg aacaaaatcc ggctccatcg cctcaagcca
     9481 gaatggcact cccgccgtcc gttccagacc gtttcccgca ccactgacca tctgcgcaaa
     9541 cacttcacca tcccgcagcc aggtccgcag cagtaaacgt tcaagcacgg gacgggtata
     9601 ctgccctgtc acatccggac tcacggacca ttcagcccac aaccggcgga tatccgcagc
     9661 cagctctgcc accatttccc cgttttttcg taatggctga ggctccacaa taattcccct
     9721 ggcaccaatc acccgctctt ccagcttgtc aaacacacca atcaccaggt catgattgat
     9781 atccagaaaa cgggcctgct cccgcaggga aaccgcaccg tatttactga gctgatcagc
     9841 agagcgattt tcccgccggg ctttatgtgt ccgggtcggt ttcaccgcct cataggccat
     9901 gattaacgcc cttgaacgca gtctggctgc tttccatccg ggggaaaaca cgcctatcac
     9961 atcatcaata attgccatta aaacctcgcc agtttaaatc ccggttttcc ccgcctgcgg
    10021 ctcaccatcg cggcaagcct gcgttcccac tcctgacgtc cggcgcggat ctgagaaagg
    10081 ctttccagcg tcagttgctg cccgttgaag gtgacagact tcccctccag tacggccatt
    10141 tccgcttcac ggtaccgctg tatcatttct ctggcttctt ctgtgctcac aaccagcctc
    10201 ctgatgttat ccatggatta tcttccgcac gctccgtccg cagttttttc ttccggcgac
    10261 ggcgtttttc tgccccggcc gtcagttccg gggataccgt ttcaccagaa cgcgcctgcg
    10321 ggaagacgag ccacgtttcc cgctgtgccc agtccggtgc ggagggccag cggatctttt
    10381 cataaccatg cagaacagca agcgcatccg cataaaccag caggtcaaac gcctcgttag
    10441 cgcccctgcc cggttttcgc cattttccgt cactgccgcg ctcttcatag gtcagctcat
    10501 cgtaaaacca ccgccccagc cagtcgggaa agtggatata gttcggccct ggtgtgtcac
    10561 gccacagggc attatttaca cggtccttaa acgcatccgt ctgaaccagc cacagcgcga
    10621 catcgccact ggctctggca cggcgggcac ttctgccggt attatccggg aaggtacggt
    10681 taatcagcct gtcacggcga agaccatccc ccttgaacag aaacaccctg ttgcccagtc
    10741 cgtcactccg gcaacgacgc cagaaacgat aggcgttatc tgtcaccccg gcttcccctc
    10801 ccgtatccac cgccatggcc atcagacgca tgcgcacatc cggatcagaa gccagcggcc
    10861 atgttttatg gaacacatcc gtcagcaaca aatcccagtc ctccggatat gccgccggat
    10921 caaccggcag actttcaccg ttgggactgc atcgcagtga atgccggatg ttgtagcgat
    10981 caacaatcca gcgttccccc tgctctccgt atccggtgat ctgcacaaca aaacggcgat
    11041 ttttaccgcc ctgtacgtca accgttgcct caataaaacg cacaccatcc ggcacagatc
    11101 gccggggaaa cggctcggca cgctgttcaa gcagttcact tttacgctgt tccgtggctg
    11161 aacggggcag atagggtcgt ccgatatcgg tgttcaccac cgctttcagg gtctcttcac
    11221 tgccggttcg ctcatactct tcttctgccg ccagcagctt aaaaatcagt tgttcccagg
    11281 tctgaaacgc cgcagccggc ccctccatcc agaaacttgc tattctggaa tttcgggggg
    11341 ttccggtgat actgccatcc gccgctgccc gttcgccttc ccgcagccag atcccctggt
    11401 tattcagttc acgcttctgt tccggagcaa tcaggccgcg gcagtgcgga cacatcaggc
    11461 gggccgcctg cccggcagcc acaaaatcag ggttattccg gtaaccggtc atgttatcca
    11521 tcaccggctg aaaatattcc ccgcagtgcg gacacggcca gtaccaccgg cggcggtctc
    11581 cccggttata cagtgacagg atccccgttg ttggcggtgc ctcatgtgcg ccaccacaac
    11641 gccatttggt atcggtgata tcccgcccgg gcgaactttc gaccagggtc atccccgagg
    11701 acataaaggt ggtggtacgc tttgaggcca gggtgaaggc atccccttcc ccgtccacgt
    11761 tttcagggaa acggtcataa tccgtcagcg ccacacgacg gtaatccgag gaggaaaata
    11821 cggtgatcga cggccagcca attttcagga atgagccgtc aagaaacatt ttatcgtgga
    11881 cgttgttgtc attacgggaa gggctgaggc gtttgcagac ctccgggctg tggcgaaacg
    11941 tcctggaaag acgcgttctg gaatgctcac gcgccttcgt ctcggtcatc tgcaccacca
    12001 gcatgtcagc cggatcacag atgatgccgt acacaatcca gccatcaatc agcccttcgg
    12061 ttttcccggt tcgcgccggt ccgacaaaca ccaccgcgtc atattcacgg gctgataatg
    12121 tattaatggg gtctatcata tagggcgtca gcgatgactc ccacggaccg gaagtattgg
    12181 ctccccgtgg tacccgcata taacgcctga tggcttccgc tactggtaac cggccaggtg
    12241 ggcgaaacag cgaggccact tcgcgccaga tatcggatgc gcggctatgg ctctcgttca
    12301 cctgattcac atatcggcct catcacaaca gtcaatgact gccttttcca gtgtgtcgcg
    12361 gatctcatca accacaatct gtacttcatt cagttgtgat gcggtccacc ccctgtccct
    12421 ctccagccgg tcaggcctgg tttccagtac ctgaactatc gctttcacca cgacagaaaa
    12481 cgaccgcctg acatcactga caggcacgag cagaccggtt tcctgctcaa atttcagtcg
    12541 gtcacgttct gactggtacc atgctttacg cgcatgagga tccatttcct cgttatctac
    12601 aggcagagga gctttcatca gttcggcaag gatatctgtc agtcggtaca gtttgagatt
    12661 gctctcatga ccaccggctg ggcttatgtt ttttacccga gccgcgacag tctgtcgatg
    12721 agcaccggat aatgcggcca gttgggaaat attcagatgc agatttttta attcacgatc
    12781 cataactccc cctgaaaatt atgtaaatac a
//
LOCUS       DAZFLD010000136        12710 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.137, whole genome shotgun sequence.
ACCESSION   DAZFLD010000136 DAZFLD010000000
VERSION     DAZFLD010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12710
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.137"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..543)
                     /locus_tag="ACHKBL_003339"
     CDS             complement(<1..543)
                     /locus_tag="ACHKBL_003339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HFO5820169.1"
                     /translation="MAFKHYDVVRAASPSDLAERLTQKLKEGWQPFGSPVAITPYTLM
                     QAIAAEGDVTTPVLVKPSDGEGTVISATSDPEYYFVVVLAGQSNGMSYGEGLPLPETY
                     DRPDPRIKQLARRSTVTPGGAACKYNDIIPADHCLHDVQDMSRLNHPKADLSKGQYGT
                     VGQGLHIAKKLLPFIPANAGI"
     gene            complement(1558..2493)
                     /locus_tag="ACHKBL_003340"
     CDS             complement(1558..2493)
                     /locus_tag="ACHKBL_003340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000231841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HFO5820170.1"
                     /translation="MVFQESNNREVARKYAEYITSVPLRRFVAEKVREYCGDLPLSIF
                     DGVCGSGQLEQFLTVGSLYAVDIQASPLLHIPDNFPDATVKTVNENFFNAIDDVSDTD
                     TFDCVIMNPPFSLQYKAQSADIREKISAMMPYKKTSGVLDDAFYVLASMKARFSFFLC
                     FPGVGYRRGELAMRQYFGNRVVELVQISGGFADTSVSVLFVIIDNEKQSDDVVVYGVG
                     FNGETCKKSAPVTEKINTENWNRPYIAEEKVVIDIVQVTAELLVRQTRRRKLEDEHNA
                     FVWSLMSDDQRTEVSRFLSVHGLFFDAGSGCVHIR"
     gene            complement(2578..2654)
                     /locus_tag="ACHKBL_003341"
     tRNA            complement(2578..2654)
                     /locus_tag="ACHKBL_003341"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2618..2620),aa:Arg,seq:tct)
     gene            complement(2668..2744)
                     /locus_tag="ACHKBL_003342"
     tRNA            complement(2668..2744)
                     /locus_tag="ACHKBL_003342"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2708..2710),aa:Arg,seq:tcg)
     gene            complement(2893..3645)
                     /locus_tag="ACHKBL_003343"
     CDS             complement(2893..3645)
                     /locus_tag="ACHKBL_003343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HFO5820171.1"
                     /translation="MNLEALPKYYSPKSPKLSDDAPATGSGGLTITDVMAAQGMVQSK
                     APLGFALFLAKVGVQDPQFAIEGLLNYAMALDNPTLNKLSEETRLQIIPYLVNFAFAD
                     YSRSAASKARCEHCAGTGFHNVLREVVKHSRSGESVIKEEWVKELCQHCHGKGEVSTA
                     CRGCKGKGIVLDEKRTRLHGAPVYKICGRCNGNRFSRLPTTLARHRVQKLIPDLTDYQ
                     WYKGYADVIDKLVTKCWQEEAYAEEQLRKVTR"
     gene            complement(3659..4648)
                     /locus_tag="ACHKBL_003344"
     CDS             complement(3659..4648)
                     /locus_tag="ACHKBL_003344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFO5820172.1"
                     /translation="MRALLTPEIAPRMGIVLFRPGSELMPLFMQGRVLLEPEPERYSS
                     FASGAVPAASQPLADDPAVRAVFRNEAVIRRAGGVECLESWLLREKGCQWPHSDWHSE
                     NMTTMRHAPGAIRLCWHCDNQLRDQFTERLESMATDNCARWVLSVVRRDLGFDDSHVV
                     TMPELCWWLIRNDLADALPESAARKALRLPNLVVPSVTRESDLVPSVPATSIIQDKAK
                     KVLALKVDPESPESFMLRPKRRRWVNEKYTRWVKTQPCACCGKPADDPHHLIGHGQGG
                     MGTKAHDLFVLPLCRKHHDELHADTVAFEEKYGSQLELIFRFIDRALAIGVLA"
     gene            complement(4656..5471)
                     /locus_tag="ACHKBL_003345"
     CDS             complement(4656..5471)
                     /locus_tag="ACHKBL_003345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005634 - nucleus [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
                     /protein_id="HFO5820173.1"
                     /translation="MNQLLVIDGVSVRQYFESNYCLNDLQKAALLAAGENRSSRSLEV
                     HEFMRRPETKALVELLEEETTGDSRSIPVITIQGRNGGTYVCKELVYAYAMWISPAFS
                     LKVIRTFDALHNSSPEETTSDKIKSGVILLESAAKTLNLSNSSKLGAYQKLSKVAGLP
                     ELMPIYAIDAPADAPDGSSRPTLSLSALLKQYGIRLTANQAYHQMAKLGIVEQRERYS
                     PTAINNIKKFWSLTAKGCMFGKNITSPANPRETQPHIFESRFPELLKLLDTVH"
     gene            complement(5634..6029)
                     /locus_tag="ACHKBL_003346"
     CDS             complement(5634..6029)
                     /locus_tag="ACHKBL_003346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000767123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFO5820174.1"
                     /translation="MKLILPFPPSVNTYWRHPNKGAFAGKSLISAAGRKFQSAACTAI
                     VEQLRRLPKPTSAPASVEIVLFPPDNRIRDLDNYNKALFDALTHAGVWEDDSQVKRML
                     VEWGPVIPEGKVEITISKYEKASCKLATR"
     gene            complement(6026..6352)
                     /locus_tag="ACHKBL_003347"
     CDS             complement(6026..6352)
                     /locus_tag="ACHKBL_003347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003878599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional regulator"
                     /protein_id="HFO5820175.1"
                     /translation="MTTLTQCQQQVLDMLISYQKERGFPPTNQEVATMLGYRSVNAAV
                     EHLRALEKKGVITIKRGVARGITLHTAVKDDDSEVAGIIRALLAGEENARLRAAHWLH
                     ERGLKV"
     gene            complement(6349..7002)
                     /locus_tag="ACHKBL_003348"
     CDS             complement(6349..7002)
                     /locus_tag="ACHKBL_003348"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001378271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage N-6-adenine-methyltransferase"
                     /protein_id="HFO5820176.1"
                     /translation="MSNKYCQALVELRNKPAHELKEVGDQWRTPDNIFWGINTLFGPF
                     VLDLFTDGDNAKCAAYYTAEDNALAHDWSERLAELKGAAFGNPPYSRASQHEGQYITG
                     MRYIMKHASAMRDKGGRYVFLIKAATSEVWWPEDADHIAFIRGRIGFELPVWFIPKDE
                     KQVPTGAFFAGAIAVFDKTWKGPAISYIGRDELEACGEAFLAQVRQQAEKLVREMAA"
     gene            complement(7002..7496)
                     /locus_tag="ACHKBL_003349"
     CDS             complement(7002..7496)
                     /locus_tag="ACHKBL_003349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HFO5820177.1"
                     /translation="MIMSLLNEVQKFIEAHPGCTSGDIADAFAGYSRQRVLQSASKLR
                     QSGRVAHRCEGDTRRHFPRLTEIPQEPEPQPVRESRPVRNFYVGTNDPRVILCLARQA
                     EELESRGLYRRAATVWMAAFRESHSQPERNNFLARREQCLRKSSKRAASGEEWYLSGN
                     YVGA"
     gene            complement(7493..8311)
                     /locus_tag="ACHKBL_003350"
     CDS             complement(7493..8311)
                     /locus_tag="ACHKBL_003350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000061490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO5820178.1"
                     /translation="MSMELMVKAMKIRVGNPLRKLVLIKLADNASDQGECWPSYQHIA
                     DQCEISKRSVMNHIAALCESGLVKKVTRKGEKGNSSNIYLLHLDGAGDSPGGSANNSL
                     SGAANSPGSAGVAPGGSAGDSPRTSHSFEPVKEPVNEPIAVGASADESVRVRSNRPEY
                     SPEFEQAWLAYPKRAGGNSKSAAFKAWKARLNEGVNPETMLEGVKRYAGWVSAMGNSG
                     TQFVKQAVTFFGPDRHFEESWEVPAVSVARREDPYFKASYDNVDYSQIPAGFRG"
     gene            complement(8308..8544)
                     /locus_tag="ACHKBL_003351"
     CDS             complement(8308..8544)
                     /locus_tag="ACHKBL_003351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000933940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5820179.1"
                     /translation="MLNPLSLNIRRLLQRKKTSIPTVGQWYTTPAGHVLRVSLVDREC
                     QKVICEPLGRNYRVSMPLITFCSGKNMKHLGGAA"
     gene            complement(8537..9247)
                     /locus_tag="ACHKBL_003352"
     CDS             complement(8537..9247)
                     /locus_tag="ACHKBL_003352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005080040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ash family protein"
                     /protein_id="HFO5820180.1"
                     /translation="MRFSEIAAVYFHALNKMLVSGTHTQWKLIDLQSDLFTVVFDSIV
                     HELKHNKIVMFAQCIDSVIPGCVDNIYLSGHTLHIVAKSTIGSETPELSTAIYDAPAS
                     FLSSALTHARIMVGWAGEPKGSPVSVDAGSLNPVQSATSEIETSGGGSFPQSTEAAIM
                     ATVPAITRLNDEDLHKLSYITTALRALRKVTLSDPQAHQVLVETILNLQAERIRLADK
                     ANFHIHCLLNISGGHRHA"
     gene            9212..9442
                     /locus_tag="ACHKBL_003353"
     CDS             9212..9442
                     /locus_tag="ACHKBL_003353"
                     /inference="COORDINATES: protein motif:HMM:NF017757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO5820181.1"
                     /translation="MKVYGSNLGEPHTCAFGDGLFELRIKGSDGIARVFYCTLTGKRI
                     IMLHSFIKKTQKTPPAERKKAETRMKEVKHDW"
     gene            9432..9740
                     /locus_tag="ACHKBL_003354"
     CDS             9432..9740
                     /locus_tag="ACHKBL_003354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO5820182.1"
                     /translation="MTGKRTPPTMTHDEMAAKWMDDPAFKAEYDAIADEFALLDEMLA
                     ARKEAGLTQAEVAERMGTKATAITRMESNLASGVSGPSFATLKKFARATGKKLQIRFV
                     "
     gene            complement(9762..9935)
                     /locus_tag="ACHKBL_003355"
     CDS             complement(9762..9935)
                     /locus_tag="ACHKBL_003355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFO5820183.1"
                     /translation="MQELNRWFRDGRGLYVHVIRWEPETQRVIYLRKDYPHECFSPLW
                     KFRRDFVECEAPP"
     gene            complement(10081..10632)
                     /gene="ymfL"
                     /locus_tag="ACHKBL_003356"
     CDS             complement(10081..10632)
                     /gene="ymfL"
                     /locus_tag="ACHKBL_003356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmfL"
                     /protein_id="HFO5820184.1"
                     /translation="MGKHHWKVEKQPEWYVKAVRKTIAALPGGYAEAADWLDVTENAL
                     FNRLRADGDQFLPLGWAMVLQRAAGTHHIADAVAQSAGGVFVSLPEIDEVENADINQR
                     LLEAIEQIGSYSKQIRSAIEDGVVEPHEQTAINDELYLSISKLQEHAALVYKIFCAPE
                     KSDARECAAPGVVAFCVCGETNA"
     gene            complement(10676..10876)
                     /locus_tag="ACHKBL_003357"
     CDS             complement(10676..10876)
                     /locus_tag="ACHKBL_003357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO5820185.1"
                     /translation="MKAYWDSLTKEQQGELAEKVGSTPGYLRLVFNGYKKASFVLAKK
                     LEQCTSGAITKSDLRPDIYPKD"
     gene            10967..11641
                     /locus_tag="ACHKBL_003358"
     CDS             10967..11641
                     /locus_tag="ACHKBL_003358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family protein"
                     /protein_id="HFO5820186.1"
                     /translation="MKTVHDIRRSNARKLRDGVGGNSSFATMIDREPTQTSRFMGDGA
                     TKNIGDSMARHIEKCFDLPVGWLDQEHQTTNITKRPDVSITNKQITLVPVISWVQAGA
                     WKEVGYSEVDLSTAETYPCPVPCGEMTYILRVIGDSMIDEYRPGDMIFVDPEVPACHG
                     DDVIALMHDTGETTFKRLIEDGTQRYLKALNPNWPEPYIKINGNCSIIGTVIFSGKPR
                     RYQIKA"
     gene            12001..12311
                     /locus_tag="ACHKBL_003359"
                     /pseudo
     CDS             12001..12311
                     /locus_tag="ACHKBL_003359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518763.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            12326..12688
                     /gene="yfdP"
                     /locus_tag="ACHKBL_003360"
     CDS             12326..12688
                     /gene="yfdP"
                     /locus_tag="ACHKBL_003360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YfdP"
                     /protein_id="HFO5820187.1"
                     /translation="MASERSTDVQAFIGELDGGVFETKIGAVLSEVASGVMNTKTKGK
                     VSLNLEIEPFDENRVKIKHKLSYVRPTNRGKISEEDTTETPMYVNRGGRLTILQEDQG
                     QLLTLAGEPDGKLRAAGH"
BASE COUNT         3209 a         3438 c         2969 g         3094 t
ORIGIN      
        1 aatgcccgca ttcgccggta taaacggcag cagttttttg gcgatatgca gcccctgccc
       61 cacggttccg tactgcccct ttgacaggtc cgctttcgga tggttaagac ggctcatgtc
      121 ctgcacatca tgcagacaat ggtccgccgg aataatgtcg ttatacttac aggcggcacc
      181 gcccggtgtc accgtactgc ggcgcgccag ctgcttaata cgcgggtccg gacggtcata
      241 tgtctccggc agcggaaggc cttcaccata cgacatgccg tttgactgcc ctgccagaac
      301 cacaacaaag taatactccg ggtcgctggt ggcgctgata actgtgcctt ctccatccga
      361 cggcttcacc aacacaggtg tggtgacatc accttccgcc gcaatggcct gcatcagggt
      421 ataaggcgtg atggccaccg ggctgccaaa tggctgccac ccctccttca gtttttgagt
      481 cagtcgctcc gcaaggtctg acggcgacgc cgccctgacc acatcgtagt gtttaaatgc
      541 catgaatcct cccggtcggg ataatgttgt gagtcagata aggggcgggc tgaagtccgg
      601 aagttacagg acaatgacag gaggaagact acagcccgca atacgaaaaa ggccgcgcag
      661 ttgcgcagcc ttatgaattc tggttaaaat ctgtccgcta aattcattgc tttttgaaag
      721 tgttaatcat gtttgcccgc acattgttgc gggctttttt atccataaga aagcccctcc
      781 ggagaggggc tggagtagcg ctatgtaccg ttcattgcat gatgccgggt gcctcccggt
      841 gagttcagcc cggtgccacc gaacccgcgt cattctcgct aacgatcatc agagaccata
      901 ccgtacacca gccgcccctc cgcacagggg gattcaccat gcagaaattt tttaacaaat
      961 tctcagtcgg gaagacaacc ttcaactgac tgaattgtga gcaacatagc atttaacggg
     1021 gaacctgttt tctgcagtaa aaaggcccac cggagcggac gggcctggaa ggatagcggt
     1081 catgtgatgc cggtttcccg gtaactcagc accggtatct gagtcaacgt tttctctact
     1141 gggtcatttc cgatacgccc tgcctgctga caggctttca tcacatctga aaatatagca
     1201 ccctgactga tactgtagta cccaaggttc cagaaactgt gatgtatccg gcacagaaaa
     1261 gcccctccgg agaggggctg acgctgcgta tctgtatcat catgcgcatg gtgccgggtg
     1321 cctcccggtg agttcagtat cagcacctga acccgcacag aaaggataag ggatgtaacc
     1381 aaacacctgt cgctgatatg cccctccgct cagggggatt caccatgcca gtttctttta
     1441 acaaactccc cgcaaatcag acaactgtca accgtctgaa ttgtgagaca tttaaaattt
     1501 tcggggcaac aggaagacaa tcatcacgac accggcaacc agcgaccggc accagattta
     1561 acgtatgtgc acacaacctg aaccggcatc aaaaaacaaa ccgtgcactg acagaaaacg
     1621 ggaaacctcc gtccgctgat catcactcat taaggaccac acaaaggcgt tatgctcatc
     1681 ttcaagtttc cgtcttcttg tttgccttac aagcaactca gccgtcacct gtacaatgtc
     1741 aatgaccacc ttctcttcag caatgtacgg cctgttccag ttctctgtgt taatcttctc
     1801 cgtcaccggc gctgactttt tgcaagtttc cccattaaac ccgacgccat aaacaacaac
     1861 atcatcgctt tgcttttcgt tatcaataat gacgaataat acagaaaccg atgtatcagc
     1921 gaacccgcct gaaatctgaa ccaactcaac aacgcggttg ccaaaatact gacgcattgc
     1981 cagctcacca cgcctgtaac caaccccggg aaagcacaaa aaaaatgaaa atcttgcctt
     2041 catgctcgcc agaacataaa acgcgtcatc cagaacaccg gaggtctttt tataaggcat
     2101 catcgctgaa atcttctccc tgatatccgc gctctgtgct ttgtactgta gcgaaaaagg
     2161 cggattcatg atgacacaat cgaacgtgtc cgtgtcactg acatcatcga tggcgttgaa
     2221 gaaattttca ttgaccgtct tcactgtggc atccgggaaa ttatccggga tatgcaacaa
     2281 cggagaggcc tgtatatcca cagcatacag acttcccacc gtgagaaact gctccagttg
     2341 cccggagcca cacacaccat caaaaatact caggggcaga tcaccacaat attcccgcac
     2401 tttttcagcg acaaaccgac gcaaaggaac ggacgtgatg tactctgcgt attttctggc
     2461 gacttcgcga ttattggatt cctgaaaaac catttgtgaa aggctctgtg gttgatatgc
     2521 gccgggcgtg gcacggagac aaaaaaggcc cgcaaaagcg agccaggtaa ataaatatgg
     2581 cgcgctgtac tggattcgaa ccagtgaccg attgcttaga aggcaattgc tctgtccggc
     2641 tgagctaaca acgcagtgta ccgataatgg accgccatcg aggactcgaa ccccgcgcaa
     2701 ccagcttcga aggctggcgc tctatcctga tgagctaatg gcggtattta acaggagatg
     2761 caacaaaaac ccgctcagcg gcgggctctt aaatcttatc aacggtagac atacaaagcc
     2821 catcgttggg aaaactttat ccatattttt tgaaaaatgc aagcatcatg tcgacatctt
     2881 cggcgaaatt atctatcttg tcacttttct caattgctct tcagcatacg cttcttcctg
     2941 ccagcacttt gtaaccagtt tatcaatgac atctgcatat cctttgtacc actgataatc
     3001 cgtcaggtct ggtatcagct tctggacacg atgcctcgcc agtgtggttg gtaaacggct
     3061 aaaccggttt ccattgcaac gcccacaaat cttataaaca ggcgcgccat gaagccgggt
     3121 tcttttttca tccaggacaa tacctttacc cttacaccct ctgcacgctg tgctgacttc
     3181 tcccttacca tggcaatgct gacatagttc cttcacccac tcttccttga taacagattc
     3241 cccgcttctg gagtgtttca ccacttcgcg caatacatta tgaaatccag taccagcaca
     3301 atgctcacag cgagccttac ttgccgcaga ccgggaataa tcagcaaagg caaaattcac
     3361 aaggtaaggg atgatctgta accgggtttc ttcactcaat ttattcaatg tcgggttatc
     3421 cagtgccatc gcgtaattga gtagaccttc aatcgcaaac tgaggatcct gaacaccgac
     3481 ttttgccagg aataaggcaa acccaagcgg tgctttcgac tgcaccatcc cctgcgcagc
     3541 catcacatct gtaatcgtta aaccacccga gcctgtcgcc ggtgcgtcat cgctcaattt
     3601 tggagatttt ggggagtaat attttggtaa ggcttcaagg ttcatgctcg ttctccactt
     3661 acgccagtac gcctattgcc agcgcacgat cgataaaacg aaatatcagc tccagctggg
     3721 agccatactt ctcttcgaat gccacggtat ccgcatgcag ctcgtcgtga tgctttctgc
     3781 acaaaggcaa cacaaagagg tcatgcgctt ttgttcccat tccaccctga ccgtgaccta
     3841 tcaggtggtg gggatcatca gcgggctttc cacaacatgc acacggctgt gtcttaaccc
     3901 agcgcgtgta cttttcatta acccagcggc gacgttttgg gcgtaacata aaagactccg
     3961 gcgactccgg atccactttc agcgccagca ccttttttgc cttatcctgg atgatgctgg
     4021 tggcaggaac cgaaggcaca aggtcacttt cccgggtgac agacggcaca acaagatttg
     4081 gtaatctcag tgccttacgg gctgcacttt ccggtaaggc atccgccaag tcattacgaa
     4141 tcagccacca gcacagttcc ggcattgtca caacgtgact atcatcaaaa ccgagatccc
     4201 gacgcacaac agacaacacc cagcgggcac agttatccgt cgccattgat tccagccgtt
     4261 ccgtgaactg atcgcgcagc tggttatcgc agtgccagca cagacggatt gcgcccggag
     4321 cgtgtcgcat tgtggtcatg ttctcgctgt gccagtcgga atgaggccac tggcagcctt
     4381 tttcacgaag taaccagctt tcaagacatt ccacgccacc agcacgacgg atcactgcct
     4441 cattgcggaa cacggcccga acggcaggat catccgccag cggttgtgat gccgccggaa
     4501 cggcaccact ggcgaaagat gaataacgct ccggctcagg ctccagcagg acacgcccct
     4561 gcataaacag gggcatcagc tctgaacctg gcctgaacaa tacgatcccc atacgcgggg
     4621 cgatttcagg ggtcagtagt gctctcacgg tcacctcaat gaacggtatc gagcagcttt
     4681 aacagctcag ggaatcggga ttcgaagata tgcggctgcg tctcgcgcgg atttgcagga
     4741 ctggtgatgt tcttgccgaa catgcagcct ttcgcggtca gcgaccagaa ttttttgatg
     4801 ttgttaatcg cggtagggct gtatcgttcg cgttgttcaa cgatccccag cttcgccatc
     4861 tggtgatatg cctgattagc cgtcaggcgg ataccatact gcttcagcag tgcactcagc
     4921 gacagcgtag ggcggcttga accatctggc gcatcagcag gtgcatcaat ggcatagatc
     4981 ggcataagtt caggaagacc agctaccttt gataatttct ggtatgcacc aagtttcgag
     5041 gagtttgaca gatttagagt ctttgctgct gattcaagca gaatgacccc ggatttaatt
     5101 ttgtcggatg tggtttcttc tggtgatgaa ttatgaagcg catcaaaagt acgtatcact
     5161 tttaagctga atgccgggct gatccacatt gcatatgcat agaccagctc tttacagaca
     5221 tacgtcccac cattgcgccc ctgaatggtg atgacaggaa tactacggga atctcccgta
     5281 gtttcttctt ccaataattc cacaagagcc ttcgtttcag gacgacgcat aaactcgtga
     5341 acttccagcg aacgggagga gcgattctca ccagcggcaa gaagagcagc tttctgaagg
     5401 tcgttaagac agtagttaga ttcgaagtac tggcgcacag aaacgccatc aattacaagc
     5461 aactgattca ttggtttctc cacaaatttt tatccacgag cgggactgca ctcccttttc
     5521 gttgatgcag gatgaactta ctgcgatttt taatagttat caaggataca ctgttcataa
     5581 atacagtatc tttaacgagg taatacccaa atttagggtg ttgctcaatt ccgttaccga
     5641 gttgctaatt tgcaactcgc tttttcgtac ttactgatag tgatctcgac cttcccctcc
     5701 gggataaccg gtccccactc caccagcatt cttttcacct gactgtcgtc ttcccacaca
     5761 cccgcgtggg tcagggcgtc aaacagcgcc ttgttatagt tgtccagatc gcggatccgg
     5821 ttatccggag gaaacaacac gatctccact gaagcaggtg ccgacgttgg tttcggcaga
     5881 cgacgtaact gctcaactat tgctgtgcac gccgcactct ggaattttcg ccccgccgcg
     5941 cttatcaggc tcttaccagc aaatgcccct ttgttggggt gtcgccagta ggtgttcacg
     6001 ctgggtggaa aaggcagaat cagcttcata ctttcagccc cctctcatgt aaccagtggg
     6061 ctgcacgcag cctggcgttt tcctcaccag caagcagtgc gcggataatc ccggccacct
     6121 cgctgtcgtc gtccttcacc gcggtatgaa gcgtgatgcc ccgggccacg ccacgcttta
     6181 tcgtgatgac gccttttttc tccagtgcgc gaagatgctc caccgctgca ttcactgaac
     6241 ggtatcccag catggttgcc acctcctgat tggttggcgg gaagccacgt tctttctgat
     6301 aagaaatcag catatccagc acctgctgct ggcattgagt taacgtcgtc atgccgccat
     6361 ctccctgacc agtttttccg cctgctggcg aacctgcgcc agaaacgcct caccacatgc
     6421 ctcaagttca tcgcgcccga tgtagctgat tgccggtccc ttccaggtct tgtcaaaaac
     6481 agcaatagca ccagcgaaaa aagctcctgt cggtacctgc ttctcgtctt tcgggataaa
     6541 ccagacaggc agttcaaaac caatacgccc gcgaataaaa gcaatatgat ctgcatcttc
     6601 cggccaccac acttcgctgg tggcagcttt gatcaggaaa acatagcgcc cgcccttatc
     6661 acgcatggca ctggcatgtt tcatgatgta acgcatgccg gtgatgtatt gcccctcatg
     6721 ctgactggcg cggctgtatg ggggattacc aaaggcagca cctttaagct ccgcaagacg
     6781 ttctgaccag tcatgcgcca gcgcgttgtc ttccgccgtg taatacgcgg cacatttggc
     6841 gttatcaccg tcggtgaaca gatccagaac aaacgggcca aacagggtgt taattcccca
     6901 gaaaatgttg tccggcgtgc gccactgatc gcccacttcc ttcagttcat gggctggttt
     6961 gttccgcagt tccaccagcg cctggcaata tttattactc attaagcccc cacgtaattc
     7021 cctgacagat accactcttc acctgatgca gcccgcttgc tgcttttccg taagcactgc
     7081 tcacgacgcg ccagaaaatt gtttcgttct ggctgggagt ggctttcacg gaatgccgcc
     7141 atccacaccg ttgcagcacg acggtataag ccccttgact ccagttcttc agcctggcgg
     7201 gccaggcaca aaatcacccg gggatcgtta gtgccgacat agaaattgcg cacaggtctg
     7261 gattcacgaa ctggttgcgg ttccggctcc tgcggtatct cagtcagccg cgggaaatgt
     7321 ctgcgtgtat ccccttcaca acggtgagcc acacgcccac tctgacgtaa cttgcttgct
     7381 gactgcagaa cgcgctgccg tgagtaacct gcaaaagcat ccgcaatgtc tccggaagta
     7441 caccccggat gggcttcaat gaatttctga acttcattca aaagactcat gatcaccccc
     7501 tgaatcctgc cgggatctgg ctgtagtcca cgttgtcgta actggctttg aagtacgggt
     7561 cctcgcgtct ggctacagat accgcaggaa cttcccagga ttcttcgaaa tgacgatccg
     7621 gaccaaagaa cgtgacagcc tgtttcacaa attgtgtgcc gctgttaccc atcgcagata
     7681 cccagcccgc gtagcgtttc acaccttcca gcatggtttc ggggtttact ccctcattca
     7741 aacgggcttt ccaggctttg aaggctgcag attttgaatt gccaccagca cgtttggggt
     7801 atgccagcca tgcctgctca aactccggag agtattccgg tcggtttgaa cgaactcgca
     7861 cagactcatc agcagatgca ccaacagcta ttggttcatt gactggttct ttgactggtt
     7921 caaaagagtg actggttctg ggtgaatctc ctgcactacc ccctggtgca actcctgcac
     7981 tacctggtga atttgctgca ccagatagtg aattatttgc actaccccct ggtgaatctc
     8041 ctgcaccatc cagatgaagg agatagatat tacttgagtt acctttttca cctttccggg
     8101 taactttttt taccagcccg gactcacaaa gggccgcaat atgattcatc acagaacgtt
     8161 tgctaatctc gcactggtca gcaatatgct ggtagctggg ccagcactcc ccctgatcgc
     8221 tggcattatc agccagcttg atcagaacca gttttcgcaa tggattaccc actcgaattt
     8281 tcatcgcttt aaccatcagc tccatactca tgctgcacct ccgagatgct tcatgttttt
     8341 tccggagcaa aaggttataa gcggcatact gacgcggtaa ttacggccca gcggttcaca
     8401 aatcactttc tgacattcac ggtcaaccag gctaacacgt agaacatgcc ctgcgggcgt
     8461 ggtgtaccac tgcccaactg taggaattga cgttttttta cgctgaagta aacggcgaat
     8521 attgaggctc aacggattaa gcatgacgat gccctccgct gatattcagg agacagtgaa
     8581 tatgaaaatt agccttatcc gccagacgaa tacgttcagc ctgcaagtta agaatggttt
     8641 ctaccagaac ctgatgcgcc tgcggatccg aaagagttac cttgcgcaga gcacgtagtg
     8701 cagttgttat ataactgagt ttatgtaagt cttcatcatt cagacgagtg atggctggga
     8761 cagtagccat gatggcagcc tccgttgact gtggaaaact tccaccaccg gaggtctcaa
     8821 tctcgctggt ggcggactgg acagggttga gactaccggc gtcaacggag accggcgagc
     8881 ctttcggctc ccctgcccag cccaccataa ttctggcgtg cgtgagcgcg gacgataaaa
     8941 aagacgctgg cgcgtcgtat atcgccgttg acaattccgg ggtctcactc ccgatagtgg
     9001 attttgccac tatatgtaaa gtgtgccccg acaaataaat attgtcaacg catcccggaa
     9061 ttacagaatc aatacattgc gcaaacataa caattttgtt atgtttcaat tcatgaacta
     9121 tactatcgaa tactacagtg aagaggtcag actggaggtc gatcagcttc cattgagtat
     9181 gcgtgcccga taccagcatc ttgttgagcg catgaaagta tacggcagca atctcggaga
     9241 acctcatacc tgcgccttcg gtgacggact tttcgagctt cggattaaag gcagtgatgg
     9301 catcgcacgc gtcttttact gcaccctgac aggaaaacgc atcatcatgc tgcatagctt
     9361 catcaagaag acccagaaaa ccccaccagc tgaacgcaaa aaagctgaaa ccagaatgaa
     9421 ggaggttaag catgactggt aaacgtaccc cgccaaccat gacacacgat gaaatggcag
     9481 ctaaatggat ggatgacccg gcctttaaag cagaatacga cgccatcgcc gacgagttcg
     9541 cactgcttga tgaaatgctg gccgcacgca aagaagctgg tctcacccag gctgaagttg
     9601 ctgaacgcat gggaactaaa gcaactgcca tcaccagaat ggaaagcaat cttgcctctg
     9661 gtgtcagtgg accatcattt gccacactga aaaaatttgc acgcgcaaca gggaaaaaac
     9721 tccagatccg cttcgtgtaa atttccatta ttgcgccgtt attatggcgg cgcttcgcac
     9781 tcaacaaaat cacgcctgaa tttccacaaa ggactaaagc actcatgcgg gtagtctttg
     9841 cgaagataga taacgcgctg tgtttctggt tcccaacgaa taacatgaac ataaagtcct
     9901 cttccgtcac gaaaccagcg gttaagttcc tgcacaactt gccccccaca gtcaggtaaa
     9961 gttctctgtg gttacttaca gccaggtgat ttggtaatct gcattcatgc cgtaacaaca
    10021 ggtgttcagc gacgctgacc accagctgtt gcgacaaacg gttatttgcc gttaaactgt
    10081 tcatgcgtta gtttctccac agacacaaaa cgccacgacg cccggagctg cacactcgcg
    10141 ggcgtcactc ttttctggag cgcaaaagat tttgtagacc agtgctgcat gctcctggag
    10201 cttcgaaatt gacagataca actcatcatt aattgctgtc tgctcgtgtg gctccactac
    10261 cccgtcttcg attgccgaac gaatctgctt tgagtaacta ccgatctgtt cgatggcttc
    10321 cagcaatcgc tggtttatat cggcgttctc tacttcatca atttcaggaa gcgatacaaa
    10381 caccccacca gcagactgtg cgacagcatc cgcaatgtgg tgagtaccag ccgcacgctg
    10441 taaaaccatt gcccatccca acggtaaaaa ctgatcgcca tcggcacgaa ggcgattaaa
    10501 taatgcgttc tctgttacat ccagccagtc agccgcttca gcgtaacccc ccggcaacgc
    10561 cgcgatagtt tttctgacag ctttcacgta ccactctggc tgtttttcta ctttccagtg
    10621 atgcttaccc acggttagcc tcatcgttct gtggttaaaa attgaaggtg ttctgttaat
    10681 ctttcggata gatatccggt cttaagtcag atttcgtaat tgcacctgac gtgcattgct
    10741 caagtttttt agccagcaca aaactggctt ttttataacc attgaaaacc agccgtaagt
    10801 agcctggtgt tgagccaact ttttcggcca actcgccctg ctgttctttg gttaaagagt
    10861 cccaatacgc tttcatacaa tatgtacctc ccgtatacat attacatgat tgagatgaac
    10921 cttcaagata cttgtacctt atcggtacaa aggttttaat ttctttatga aaacagtcca
    10981 tgacatccgg cggtctaacg ccagaaaact gagagatggt gttggcggga attcttcctt
    11041 tgccaccatg attgatcgcg agccaaccca gaccagcagg tttatgggag atggtgctac
    11101 taaaaatatc ggtgacagca tggcacggca catcgaaaaa tgtttcgacc tgcctgtcgg
    11161 atggcttgat caagaacacc agaccacgaa catcacaaaa agacctgatg tttcaatcac
    11221 taataaacaa ataacgttag tccctgtcat atcatgggta caggccggag catggaaaga
    11281 agttggctat tctgaggttg atttgagcac agcagaaact tatccctgcc ctgtaccctg
    11341 tggcgaaatg acttatatct tgcgggtgat tggtgattca atgattgatg agtaccgccc
    11401 gggagacatg atttttgttg atcccgaagt ccctgcctgc cacggtgacg acgttattgc
    11461 attgatgcac gatacaggcg aaaccacctt caagcgattg atagaagatg gaacacagcg
    11521 ttatctcaaa gcattaaacc caaactggcc tgagccttac attaagatta acggtaattg
    11581 ctctataatt ggtacagtga ttttctcggg aaaaccaaga agataccaaa taaaagccta
    11641 atcaatattt ataacctgct tcggcaggtt tttttatact tgacaatgta cccgatagat
    11701 acataatgta tctaaaagaa acatgccgca ggcgagattc aaacaaagtt tggttgtaac
    11761 acggcgtatg gcacatgcgt cgttagcggt ctggggacgt taaaggggac aatccactcc
    11821 ttgctcgggc aaacaaacca ggtagccgga atgtgcaagt caatgatgat gctgataaga
    11881 cgcctaacca gcgtggcgat tcggtttgac gcctgggaag agaccagggt gcaacgatga
    11941 gggaatttat ggaaccgcga caaagagtgg tgccgtaact ggctaagtgc tctcagcgtt
    12001 gtggtgaatg cggctcagcg cacgcgggtt aaggttgagt ctgacagtcg accttctgtg
    12061 gatacccacc cgtctggtgt gcaaccttcg ccaggcaccg ggaggcaccc ggcaccacaa
    12121 ctttatgctg tgtgtagtcc tggcggtacc agtttgtaca cttgcttccg gctggtaccg
    12181 ctctttttac aaaacagaga agagcatcac cggacgacgg gctcataacc caatccatcc
    12241 gggcggctgc caccgcaggt gttcttctct gttttgtgga gaaaccaacc gaccttgcag
    12301 ggtcgatatg atgaggagca gcaaaatggc tagcgaacgc agtactgatg tgcaggcatt
    12361 tatcggggag ctggacggcg gcgtatttga aaccaaaatc ggcgcagttc tcagtgaagt
    12421 cgcttccggt gtgatgaaca cgaaaaccaa aggtaaggtc tcactcaacc tggaaatcga
    12481 accatttgat gagaaccgtg tgaaaatcaa acacaaactc tcatatgttc gcccgactaa
    12541 ccgcgggaaa atttccgaag aagacaccac cgaaacgccg atgtatgtca atcgcggtgg
    12601 tcgcctgact attctgcagg aagaccaggg acaattactg actcttgccg gtgaacctga
    12661 cggaaaactc cgcgcagcag gtcattaata tcgttcttaa ttaactgatt
//
LOCUS       DAZFLD010000137        12678 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.138, whole genome shotgun sequence.
ACCESSION   DAZFLD010000137 DAZFLD010000000
VERSION     DAZFLD010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12678
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.138"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            48..1562
                     /gene="caiT"
                     /locus_tag="ACHKBL_003361"
     CDS             48..1562
                     /gene="caiT"
                     /locus_tag="ACHKBL_003361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine/gamma-butyrobetaine antiporter"
                     /protein_id="HFO5820188.1"
                     /translation="MKNEKRKTGIEPKVFFPPLIIVGILCWLTVRDLDAANVVINAVF
                     SYVTNVWGWAFEWYMVVMLFGWFWLVFGPYAKKRLGNEPPEFSTASWIFMMFASCTSA
                     AVLFWGSIEIYYYISTPPFGLEPNSTGAKELGLAYSLFHWGPLPWATYSFLSVAFAYF
                     FFVRKMEVIRPSSTLVPLVGEKHAKGLFGTIVDNFYLVALIFAMGTSLGLATPLVTEC
                     MQWLFGIPHTLQLDAIIITCWIILNAICVACGLQKGVRIASDVRSYLSFLMLGWVFIV
                     SGASFIMNYFTDSVGMLLMYLPRMLFYTDPIAKGGFPQGWTVFYWAWWVIYAIQMSIF
                     LARISRGRTVRELCFGMVLGLTASTWILWTVLGSNTLLLIDKNIINIPNLIEQYGVAR
                     AIIETWAALPLSTATMWGFFILCFIATVTLVNACSYTLAMSTCREVRDGEEPPLLVRI
                     GWSILVGIIGIVLLALGGLKPIQTAIIAGGCPLFFVNIMVTLSFIKDAKQNWKD"
     gene            1593..2735
                     /gene="caiA"
                     /locus_tag="ACHKBL_003362"
     CDS             1593..2735
                     /gene="caiA"
                     /locus_tag="ACHKBL_003362"
                     /EC_number="1.3.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA dehydrogenase"
                     /protein_id="HFO5820189.1"
                     /translation="MDFNLNDEQELFVAGIRELMASENWEAYFAECDRDSVYPERFVK
                     ALADMGIDSLLIPEEHGGLDAGFVTLAAVWMELGRLGAPTYVLYQLPGGFNTFLREGT
                     QEQIDKIMAFRGTGKQMWNSAITEPGAGSDVGSLKTTYTRRNGKIYLNGSKCFITSSA
                     YTPYIVVMARDGASPDKPVYTEWFVDMSKPGIKVTKLEKLGLRMDSCCEITFDDVELD
                     EKDMFGREGNGFNRVKEEFDHERFLVALTNYGTAMCAFEDAARYANQRVQFGEAIGRF
                     QLIQEKFAHMAIKLNSMKNMLYEAAWKADNGTITSGDAAMCKYFCANAAFEVVDSAMQ
                     VLGGVGIAGNHRISRFWRDLRVDRVSGGSDEMQILTLGRAVLKQYR"
     gene            2863..4080
                     /gene="caiB"
                     /locus_tag="ACHKBL_003363"
     CDS             2863..4080
                     /gene="caiB"
                     /locus_tag="ACHKBL_003363"
                     /EC_number="2.8.3.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine CoA-transferase"
                     /protein_id="HFO5820190.1"
                     /translation="MDHLPMPKFGPLAGLRVVFSGIEIAGPFAGQMFAEWGAEVIWIE
                     NVAWADTIRVQPNYPQLSRRNLHALSLNIFKDEGREAFLKLMETTDIFIEASKGPAFA
                     RRGITDEVLWQHNPKLVIAHLSGFGQYGTEEYTNLPAYNTIAQAFSGYLIQNGDVDQP
                     MPAFPYTADYFSGLTATTAALAALHKVRETGKGESIDIAMYEVMLRMGQYFMMDYFNG
                     GEMCPRMTKGKDPYYAGCGLYKCADGYIVMELVGITQIAECFKDIGLAHLLGTPEIPE
                     GTQLIHRIECPYGPLVEEKLDAWLAAHTIAEVKERFAELNIACAKVLTVPELESNPQY
                     VARESITQWQTMDGRTCKGPNIMPKFKNNPGQIWRGMPSHGMDTAAILKNIGYSENDI
                     QELVSKGLAKVED"
     gene            4153..5706
                     /gene="caiC"
                     /locus_tag="ACHKBL_003364"
     CDS             4153..5706
                     /gene="caiC"
                     /locus_tag="ACHKBL_003364"
                     /EC_number="6.2.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016405 - CoA-ligase activity [Evidence
                     IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetaine/carnitine-CoA ligase"
                     /protein_id="HFO5820191.1"
                     /translation="MDIIGGQHLRQMWDDLADVYGHKTALICESSSGVVNRYSYLELN
                     QEINRTANLFYVLGIRKGDKVALHLDNCPEFIFCWFGLAKIGAIMVPINARLLREEST
                     WILQNSQACLLVTSAQFYPMYQQIQQEDASQLRHICLTDMVLPADDGVSSFTQRKNQQ
                     PATLCYAPPLSTDDTAEILFTSGTTSRPKGVVITHYNLRFAGYYSAWQCALRDDDVYL
                     TVMPAFHIDCQCTAAMAAFSAGATFVLVEKYSARAFWGQVQKYRATITECIPMMIRTL
                     MVQPPSANDRQHCLREVMFYLNLSEQEKDAFCERFSVRLLTSYGMTETIVGIIGDRPG
                     DKRRWPSIGRAGFCYEAEIRDDHNRPLPAGELGEICIKGVPGKTIFKEYFLNPKATAK
                     VLEADGWLHTGDTGYRDEEGFFYFVDRRCNMIKRGGENVSCVELENIIATHPKIQDIV
                     VVGIKDSIRDEAIKAFVVLNEGETLSEEEFFCFCEQNMAKFKVPSYLEIRKDLPRNCS
                     GKIIRKNLK"
     gene            5815..6600
                     /gene="caiD"
                     /locus_tag="ACHKBL_003365"
     CDS             5815..6600
                     /gene="caiD"
                     /locus_tag="ACHKBL_003365"
                     /EC_number="4.2.1.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA hydratase"
                     /protein_id="HFO5820192.1"
                     /translation="MSESLHLTRNGSILEITLDRPKANAIDAKTSFEMGEVFLNFRDD
                     PQLRVAIITGAGEKFFSAGWDLKAAAEGEAPDADFGPGGFAGLTEIFNLDKPVIAAVN
                     GYAFGGGFELALAADFIVCADNASFALPEAKLGIVPDSGGVLRLPKILPPAIVNEMVM
                     TGRRMGAEEAQRWGIVNRVVSQAELMDNARELAQQLVNSAPLAIAALKEIYRTTSEMP
                     VEEAYRYIRSGVLKHYPSVLHSEDAIEGPLAFAEKRDPVWKGR"
     gene            6606..7196
                     /gene="caiE"
                     /locus_tag="ACHKBL_003366"
     CDS             6606..7196
                     /gene="caiE"
                     /locus_tag="ACHKBL_003366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine operon protein CaiE"
                     /protein_id="HFO5820193.1"
                     /translation="MSYYAFEGLIPVVHPTAFVHPSAVLIGDVIVGAGVYIGPLASLR
                     GDYGRLIVQAGANIQDGCIMHGYCDTDTIVGENGHIGHGAILHGCVIGRDALVGMNSV
                     IMDGAIIGEESIVAAMSFVKAGFRGEKRQLLMGTPARAVRSVSDDELHWKRLNTKEYK
                     DLVGRCHASLHETQPLRQMEENRPRLQGTTDVTPKR"
     gene            complement(7315..7710)
                     /gene="caiF"
                     /locus_tag="ACHKBL_003367"
     CDS             complement(7315..7710)
                     /gene="caiF"
                     /locus_tag="ACHKBL_003367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine metabolism transcriptional regulator
                     CaiF"
                     /protein_id="HFO5820194.1"
                     /translation="MCEGYVEKPLYLLIAEWMMAENRWVIAREISIHFDIEHSKAVNT
                     LTYILSEVTEISCEVKMIPNKLEGRGCQCQRLVKVVDIDEQIYARLRNNSREKLVGVR
                     KTPRIPAVPLTELNREQKWQMMLSKSMRR"
     gene            complement(7971..11192)
                     /gene="carB"
                     /locus_tag="ACHKBL_003368"
     CDS             complement(7971..11192)
                     /gene="carB"
                     /locus_tag="ACHKBL_003368"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HFO5820195.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALEL
                     ERQGVLEEFGVTMIGATADAIDKAEDRRRFDVAMKKIGLETARSGIAHSMEEALAVAA
                     EVGFPCIIRPSFTMGGSGGGIAYNREEFEEICARGLDLSPTKELLIDESLIGWKEYEM
                     EVVRDKNDNCIIVCSIENFDAMGIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIG
                     VETGGSNVQFAVNPKNGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LMNDITGGRTPASFEPSIDYVVTKIPRFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQ
                     ESLQKALRGLEVGATGFDPKVSLDDPEALTKIRRELKDAGAERIWYIADAFRAGLSVD
                     GVFNLTNIDRWFLVQIEELVRLEEKVAEVGITGLNADFLRQLKRKGFADARLAKLAGV
                     REAEIRKLRDQYDLHPVYKRVDTCAAEFATDTAYMYSTYEEECEANPSTDREKIMVLG
                     GGPNRIGQGIEFDYCCVHASLALREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLE
                     DVLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVPVIGTSPDAIDRAEDRERFQHAV
                     ERLKLKQPANATVTTIEMAVEKAKEIGYPLVVRPSYVLGGRAMEIVYDEADLRRYFQT
                     AVSVSNDAPVLLDHFLDDAVEVDVDAICDGEMVLIGGIMEHIEQAGVHSGDSACSLPA
                     YTLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLIEVNPRAARTVPFVSKA
                     TGVPLAKVAARVMAGKSLAEQGVTKEVIPPYYSVKEVVLPFNKFPGVDPLLGPEMRST
                     GEVMGVGRTFAEAFAKAQLGSNSTMKKHGRALLSVREGDKERVVDLAAKLLKQGFELD
                     ATHGTAIVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIINTTSGRRAIEDSRVIR
                     RSALQYKVHYDTTLNGGFATAMALNADATEKVISVQEMHAQIK"
     gene            complement(11210..12358)
                     /gene="carA"
                     /locus_tag="ACHKBL_003369"
     CDS             complement(11210..12358)
                     /gene="carA"
                     /locus_tag="ACHKBL_003369"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HFO5820196.1"
                     /translation="MIKSALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILT
                     DPSYSRQIVTLTYPHIGNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYL
                     KRHNIVAIADIDTRKLTRLLREKGAQNGCIIAGDNPDAALALEKARAFPGLNGMDLAK
                     EVTTAEAYSWTQGSWTLTGGLPEAKKEDELPFHVVAYDFGAKRNILRMLVDRGCRLTI
                     VPAQTSAEDVLKMNPDGIFLSNGPGDPAPCDYAITAIQKFLETDIPVFGICLGHQLLA
                     LASGAKTVKMKFGHHGGNHPVKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFD
                     GTLQGIHRTDKPAFSFQGHPEASPGPHDAAPLFDHFIELIEQYRKTAK"
BASE COUNT         2975 a         3294 c         3363 g         3046 t
ORIGIN      
        1 aaacgggaac gcaaaataaa tgttcgtttt cacagtggaa ttaactcatg aagaatgaaa
       61 agagaaaaac gggaatagaa ccgaaggttt tctttccgcc gttaataatc gtcggcatac
      121 tttgttggct tacagtcaga gatctggatg cggcgaatgt cgttattaat gctgtattca
      181 gttacgtcac caatgtatgg ggatgggcat ttgaatggta tatggtggtg atgcttttcg
      241 gctggttctg gctggtgttt ggcccgtatg ccaaaaagcg tttaggtaac gaaccaccag
      301 aatttagtac cgccagttgg atctttatga tgttcgcctc ctgtacgtct gctgccgtac
      361 tgttctgggg atcgattgag atctactact acatctccac cccgccgttt ggcttagaac
      421 cgaactcaac aggcgcgaaa gagttggggc tggcttacag cttgttccac tggggacctc
      481 tgccgtgggc cacttacagc ttcctttcag tcgccttcgc ttacttcttc tttgtccgca
      541 aaatggaagt gattcgcccc agctccaccc tggtgccgct ggtaggtgaa aaacacgcca
      601 aagggttgtt cggcactatc gtcgacaact tctatctcgt cgccttgatc ttcgcgatgg
      661 gtaccagtct gggccttgcc acgccgctgg tgaccgagtg tatgcaatgg ttgtttggca
      721 ttccgcatac cctgcaactg gacgctatca tcattacctg ctggattatc ctcaacgcca
      781 tttgcgtcgc ttgcggtctg caaaaagggg tacgtatcgc cagtgacgtg cgtagttacc
      841 tgagcttcct gatgctgggt tgggtgttca tcgtcagcgg tgccagcttc atcatgaact
      901 acttcaccga ttcggtgggg atgttgctga tgtatctgcc gcgcatgttg ttctataccg
      961 atcccatcgc taaaggcggc ttcccgcagg gctggaccgt gttctactgg gcatggtggg
     1021 tgatttacgc tatccagatg agcatcttcc tcgcccgcat ctcccgtggt cgtaccgtgc
     1081 gtgaactgtg cttcggcatg gtgctggggc tgacagcgtc aacctggatc ctgtggactg
     1141 tactcggtag taacactctg ctgttgatag ataaaaacat catcaacatt ccaaatctga
     1201 tcgaacagta cggtgtggcg cgcgccatca tcgaaacctg ggccgctctg ccactcagca
     1261 ccgccaccat gtggggcttc ttcatcctct gctttattgc caccgttacg ctggttaacg
     1321 cctgctctta taccctggcg atgtccactt gccgcgaagt acgcgatggt gaagaaccac
     1381 cgctgctggt gcgtatcggc tggtcaattc tggttggcat tatcggtatt gttctgctgg
     1441 cgctcggcgg cctgaaaccg attcaaaccg ccattatcgc cggaggatgc ccgctgttct
     1501 tcgtcaacat tatggtgacg ctctccttta ttaaagacgc gaaacagaac tggaaagatt
     1561 aattaacccc caaaatatca agaggttgaa agatggattt taatttaaat gatgagcagg
     1621 aactgtttgt cgccggtatc cgcgaactga tggccagcga aaactgggag gcctattttg
     1681 ccgagtgcga ccgtgacagc gtctacccgg aacgttttgt caaagcactg gcggatatgg
     1741 gtatcgacag tctgctgatt cctgaagagc acggtggtct tgacgcgggt tttgttactc
     1801 tcgccgccgt gtggatggag ctgggacgtc tgggggcacc aacctatgtg ctgtaccagt
     1861 tgccgggcgg gttcaatacc ttcctgcgcg aaggcacaca agagcagatc gacaagatta
     1921 tggctttccg cggcaccggt aagcagatgt ggaactcagc gattaccgaa ccgggcgcgg
     1981 gctccgacgt gggtagcctg aaaacgactt atacccgtag aaatggtaag atttatctta
     2041 atggtagtaa gtgttttatt accagcagcg cctacacccc gtacatcgtg gtgatggcgc
     2101 gcgacggggc ttctccggac aaacctgtct acaccgaatg gtttgttgat atgagcaaac
     2161 cgggcatcaa agtgaccaaa cttgagaagc tcggtctgcg tatggatagc tgctgtgaaa
     2221 tcacttttga cgacgtggaa ctggacgaga aagacatgtt cggtcgggaa ggtaacggat
     2281 ttaaccgcgt caaagaagag ttcgaccatg aacgtttcct ggtagccctg accaactacg
     2341 gtacggcgat gtgcgccttt gaagatgcgg cgcgctacgc caaccagcgc gtgcagtttg
     2401 gcgaggctat tggtcgtttc cagttgattc aggaaaaatt cgcccacatg gcgatcaaat
     2461 taaactccat gaaaaacatg ctgtatgaag cagcgtggaa agcagacaac ggcaccatca
     2521 cctctggcga tgcagcgatg tgcaaatact tctgcgccaa tgcggcattt gaagtggtgg
     2581 atagcgcaat gcaggtgctg ggcggtgtcg ggattgcggg caaccaccgc atcagccgct
     2641 tctggcgtga cctgcgtgta gaccgcgttt ccggaggatc tgacgaaatg cagatcctga
     2701 cgctgggtcg cgcggtgctg aagcaatacc gttaagtccg tgcgctgcct gatgcgacgc
     2761 tgacgcgtct tatcaggcct acgggagcac gaagatggcc ggataaggcg tttacgccgc
     2821 atccggcagt catgcgctac ataaattttt caggagctaa taatggatca tctacccatg
     2881 ccgaaattcg ggccgctggc cggattacgc gttgtcttct ccggtatcga aatcgccggg
     2941 ccgttcgccg gacaaatgtt cgcagaatgg ggggcggaag ttatctggat cgagaacgtc
     3001 gcctgggccg acaccattcg cgttcaaccg aactacccgc agctctcccg ccgcaatttg
     3061 catgcgctgt cgttaaatat tttcaaagat gaagggcgcg aagcgtttct gaaattaatg
     3121 gaaaccaccg atatcttcat cgaagccagt aaaggtccgg cctttgcccg tcgtggcatt
     3181 accgatgaag tcctgtggca gcacaaccca aaactggtta tcgctcacct ttccggtttt
     3241 ggtcagtatg gcaccgagga gtacaccaat cttccggcct ataacactat cgcccaggcc
     3301 ttcagtggtt acctgattca gaacggtgat gttgaccagc caatgcctgc cttcccgtat
     3361 accgccgatt acttttctgg cctgaccgcc accacggcgg cgcttgcagc actgcataaa
     3421 gtgcgtgaaa ccggcaaagg cgaaagcatc gacatcgcca tgtatgaagt gatgctgcgt
     3481 atgggccagt acttcatgat ggattacttc aacggcggcg aaatgtgccc gcgcatgacc
     3541 aaaggtaaag atccctacta cgccggttgc ggtctgtata aatgcgccga cggctacatc
     3601 gtgatggaac tggtgggcat tactcaaatt gcagagtgct ttaaagatat tggcctcgca
     3661 cacctgctcg gtacgccgga aatcccggaa ggcactcagc ttatccaccg tatcgaatgc
     3721 ccttacggcc cactggtaga agagaaactc gatgcctggc tggcggcaca taccatcgca
     3781 gaagttaaag aacgctttgc cgaactgaat atcgcctgtg ccaaagtact gaccgtaccg
     3841 gaactggaaa gcaatccaca gtatgtggct cgcgaatcaa tcactcagtg gcaaacgatg
     3901 gatggtcgca cctgcaaagg gccgaatatc atgccgaaat tcaaaaataa ccccggacaa
     3961 atctggcgcg gaatgccctc acatggcatg gacacggctg ctattttgaa aaatatcggc
     4021 tacagcgaaa acgacattca ggagttggtc agcaaaggtc tggccaaagt tgaggactaa
     4081 acgcgtggcg tcagtcagct tgcctgacta gcgcccgctc aataacaaac gaacaaaatg
     4141 gatagaggtg caatggatat cattggcgga caacatctac gtcaaatgtg ggacgatctg
     4201 gcggacgttt acggtcataa aacggcgctg atttgtgaat ccagcagcgg agtcgttaac
     4261 cggtatagtt atcttgagtt aaatcaggag attaaccgca cggcaaacct gttttatgtg
     4321 ctggggattc gcaaaggcga caaggttgct ctacatctcg acaactgccc ggaatttatc
     4381 ttttgctggt tcgggttggc aaaaattggc gcgattatgg tgccgattaa cgcccgcctg
     4441 ttgcgcgaag aaagcacgtg gatcctgcaa aatagccagg cgtgcctgct ggtgaccagt
     4501 gcgcaatttt atcctatgta tcaacagatt cagcaggaag atgcctctca gttgcggcac
     4561 atttgcctga cagatatggt acttcccgct gatgatggcg tgagttcgtt tactcaacgg
     4621 aaaaatcaac aacctgccac cttgtgctat gcaccgccgc tatcgactga cgatacggcg
     4681 gaaattctct tcacctccgg caccacctcc cgaccgaaag gggtggtgat aactcattac
     4741 aacctgcgct tcgctggata ttactccgcc tggcagtgtg cactgcgtga cgatgacgtc
     4801 tacctgacgg taatgcctgc gtttcatatc gattgccagt gtactgcggc gatggcggcg
     4861 ttttctgccg gggccacctt tgtgctggtc gagaaataca gcgcccgcgc cttctgggga
     4921 caggtgcaga agtaccgcgc caccattacc gaatgtattc cgatgatgat tcgtacgttg
     4981 atggtgcagc cgccttcagc caacgatcgg caacactgcc tgcgggaagt gatgttttat
     5041 ctcaacctgt cggagcagga aaaagacgcg ttttgtgaac gcttcagcgt tcgcttgctg
     5101 acgtcttatg ggatgacgga aaccattgtg ggcattatcg gcgatcgccc tggcgataaa
     5161 cggcgctggc cgtcgattgg tcgggcgggc ttttgctacg aagcggagat ccgcgacgat
     5221 cacaatcgtc ccctcccggc tggtgagctc ggtgaaatct gtattaaagg cgtaccaggg
     5281 aaaaccatct tcaaagagta ttttctcaac ccgaaagcca ctgcgaaagt gctggaagcc
     5341 gatggctggc tacataccgg cgataccgga taccgcgacg aagagggctt tttttatttc
     5401 gtcgatcgcc gctgcaatat gatcaaacgc ggtggcgaga atgtctcctg cgtggagctc
     5461 gaaaatatca tcgccaccca tccaaaaatt caggatatcg tggttgtggg tattaaagat
     5521 tcgattcgcg atgaagccat caaagcattt gtggtgctga atgaaggtga aacattgagc
     5581 gaagaggaat tcttctgctt ctgtgaacag aatatggcga aatttaaagt gccctcttat
     5641 ctggagatca gaaaagatct gccacgtaat tgctcgggga aaataattag aaagaatctg
     5701 aaataaatga aacggcaggg aactaccctg tcagctaata atcacgctct aaaacaatat
     5761 gcattttttg cagggatgtt gtcgtccctg aaaaagcaaa aatggagaaa aggaatgagt
     5821 gaatcattac atctgacccg caatggatca attctggaaa ttacccttga tcgtccaaaa
     5881 gcgaatgcta ttgatgcaaa aaccagcttt gaaatgggcg aagtatttct aaatttccgt
     5941 gacgatccgc aattacgtgt cgccattatt accggtgccg gagagaaatt cttttccgcg
     6001 ggctgggatt taaaagcggc agcagaaggc gaagcaccgg atgctgactt tggcccggga
     6061 ggttttgcgg gattaaccga aattttcaat ctcgacaaac cggttatcgc agctgtgaac
     6121 ggctacgcct ttggcggcgg tttcgaactg gcgctggcgg cagattttat tgtttgtgcc
     6181 gataatgcca gcttcgccct gccggaagcc aaactgggca tcgttcctga cagcggcggc
     6241 gtgctgcgtc tgccgaagat cctgccgcct gccatcgtca atgaaatggt gatgaccggc
     6301 agacgaatgg gcgcagaaga ggcgcagcgt tgggggatag tcaaccgcgt ggttagccag
     6361 gcggaactga tggataacgc ccgcgaactg gcgcagcagc tggttaacag cgctccactg
     6421 gcgattgcgg cgctgaaaga gatctaccgc accaccagcg aaatgccggt agaagaagcg
     6481 tatcgctata ttcgcagcgg cgtgttgaaa cactatccat cggttctgca ttcggaagat
     6541 gccattgaag ggccgctggc gtttgccgag aagcgcgatc cggtgtggaa aggacgttaa
     6601 caaccgtgag ctattacgcc tttgagggat taattccggt ggttcacccg acggcgtttg
     6661 tccatcccag tgcagtattg attggcgatg tgattgtggg agccggtgtc tacatcggcc
     6721 cactcgcctc actgcgtggt gactacgggc ggttgatcgt gcaagcggga gccaatattc
     6781 aggatggctg cattatgcat ggctactgcg acactgacac catcgttggg gaaaacggcc
     6841 atatcgggca cggagcgatc ctgcatggtt gtgtgattgg tcgcgatgca ctggtcggga
     6901 tgaacagcgt gattatggat ggcgcgatca ttggcgaaga gagcattgtt gccgctatga
     6961 gctttgtcaa agcgggcttt cgcggcgaga aacgccagtt gttgatgggt acgccagccc
     7021 gcgctgtacg cagcgtgagt gacgacgagt tacactggaa acggttgaat accaaagagt
     7081 ataaggatct tgtcgggcgc tgtcatgcat cattacatga aacgcagcca ctgaggcaaa
     7141 tggaggaaaa tcgcccccgt ttgcagggga cgacggatgt gacgccgaaa cggtgatctt
     7201 tccggatgcg gcgtaaacgc cttatccgac ctggctggaa aggaaccgta ggccgtataa
     7261 gatgcgccag catcgcatcc ggcgaccagg tcgcacggta tcaacgaaac gacattaacg
     7321 acgcatactc tttgacaaca tcatctgcca cttctgctcg cggttaagtt ccgtgagcgg
     7381 aacggcagga atacgtggcg tctttcttac gccgactaat ttttcccgac tgttattgcg
     7441 caggcgcgcg taaatttgct catcgatatc gaccacttta accagtcgct gacactggca
     7501 tccccgccct tccagcttat tagggatcat cttaacttcg cagcttattt ctgtgacttc
     7561 cgacagaata taagtcaggg tattaaccgc cttgctgtgt tcaatatcga aatgaataga
     7621 gatctctctt gctatcaccc accgattttc agccatcatc cattcggcga ttaacaagta
     7681 gagtggtttt tcaacatatc cttcacacat acgaactcca gtttcacaaa ttaaaagtct
     7741 ggctatgact cgttttgcag caattaaaca aggccatatt aaaattgtgg atatatcgat
     7801 agtatgcaac ttcaccgtcg ataagtgtga gctaaatcct gcttatgtca tcaaaaatat
     7861 cgattcccca aaagatttac ttaatgggca aaaaaaacgg tcctcatcag agaaccgtat
     7921 tagttattcg atcaaattag cggatgaaaa atatccgcca tgacacgcta ttatttgatc
     7981 tgcgcgtgca tttcctgcac cgaaattact ttttcagtcg catcggcatt cagcgccatc
     8041 gcggtagcga aaccaccgtt cagggtggtg tcgtaatgca ctttatattg cagcgcactg
     8101 cgacggatca cgcgggagtc ttcaatcgca cgacgacctg aggtggtgtt gatgatgtag
     8161 gtatattcgc cattcttgat acggtcctga atgtgcggac gaccttcatg caccttgttc
     8221 accagacgcg gattaatgcc tgcttcgccc agcacaatcg ccgtgccgtg ggtcgcatcc
     8281 agctcgaagc cctgtttcag cagttttgcc gccaggtcca ccacgcgttc tttatcgcct
     8341 tcgcgcacgg aaagcaacgc acgaccgtgt ttcttcatgg tggagttgct gcccagctgc
     8401 gctttggcga acgcttcagc gaaggtgcgg cccacgccca tgacttcccc ggtagagcgc
     8461 atttctggcc ctaacagcgg gtcaacgccc gggaatttat tgaacggcag caccacttct
     8521 ttcaccgagt agtacggcgg gataacttct ttggttacgc cctgctcagc cagcgatttg
     8581 ccagccatca cacgcgccgc cacttttgcc agcggtacgc cggtggcttt ggagacgaac
     8641 ggaacggtac gcgccgcacg cgggttaact tcaatcaggt agacttcgtt gtttttcacc
     8701 gcaaactgca cgttcatcag gccgcgcacc tgcaattcga aggccagttt ctgcacctgc
     8761 tggcgcatca catcctgaat ttcctgactt aaagtgtagg ctggtagcga acatgcagag
     8821 tcaccggagt gcacgcccgc ctgctcgatg tgctccatga tgccgccaat cagcaccatt
     8881 tcaccgtcgc agatggcatc cacgtcaact tctactgcgt catcaaggaa gtggtccagc
     8941 aacactggcg catcgttaga tacgctgacc gctgtctgga agtagcgacg caggtcagct
     9001 tcgtcataga cgatttccat cgcccgaccg ccgagaacgt aagacggacg caccaccagc
     9061 gggtagccaa tctctttcgc tttctcaacc gccatttcga tggtggtaac ggtggcgttc
     9121 gccggttgtt tcagtttcag acgctcaacc gcatgctgga agcgttcacg gtcttccgca
     9181 cggtcgatgg catccgggct ggtaccgata accggtacgc cagcagcttc cagcgcgcgc
     9241 gccagtttca gcggggtctg accgccgtac tggacgataa cgcctttcgg cttctcaata
     9301 cgcacaattt ccagcacgtc ttccagagtt accggctcga agtagagacg gtcagaggtg
     9361 tcgtagtcgg tggagacggt ttccgggtta cagttaacca taatggtttc ataaccatct
     9421 tcgcgcagcg ccagcgaggc gtgtacacag cagtagtcga attcgatacc ctgaccgata
     9481 cggttcgggc cgccgccgag caccatgatt ttttcacggt cggtagacgg attcgcttcg
     9541 cactcttctt cataagtgga gtacatgtaa gcggtgtcgg tggcgaactc tgccgcacag
     9601 gtatccacgc gcttataaac cgggtgcaag tcatactggt cgcgcagctt gcggatttct
     9661 gcttcacgca cgcccgcgag ttttgccaga cgcgcgtcgg caaagccttt gcgtttcagc
     9721 tggcgcagga agtcagcgtt caggccagtg atgcccactt ctgcgacttt ctcttccaga
     9781 cgcaccagct cttcaatttg taccaggaac cagcggtcaa tgttggtcag gttgaacacg
     9841 ccgtctacgg acaggcccgc gcggaaagca tcggcgatat accagatacg ctctgcgcct
     9901 gcatctttca gttcgcggcg aattttggtc agcgcttccg gatcatcgag gctgactttc
     9961 gggtcgaagc cagttgcgcc cacttccagg ccgcgcagcg ctttttgcag ggattcctgc
    10021 tgcgtgcgac caatcgccat cacttcgcca accgatttca tctgagtggt cagacggtcg
    10081 ttcgccccgg cgaatttctc gaagttgaag cgaggaattt tagtcaccac atagtcgatg
    10141 gacggctcga aggaggccgg agtacgccca ccggtgatgt cgttcatcag ctcgtcgagg
    10201 gtgtaaccca ccgccagttt cgccgccact ttggcaatcg ggaaaccagt ggctttcgac
    10261 gccagcgcag aggagcgaga cacgcgcggg ttcatttcga taacaatcag gcgaccgttt
    10321 ttcgggttca ccgcaaactg gacgttggaa ccaccggttt caacgccgat ttcacgcagt
    10381 accgccatcg aggcgttacg catgatttga tattctttgt cggtcagcgt ttgggctggc
    10441 gcgacagtga tggagtcacc ggtgtggatg cccatcgcat caaagttttc gatagagcag
    10501 acaatgatgc agttgtcgtt tttatcacgc accacttcca tctcgtactc tttccagccg
    10561 atcagcgact catcaatcag cagctcttta gtcggagaga gatccagacc acgggcgcaa
    10621 atttcttcaa actcttcgcg gttataagcg ataccgccgc cgctaccgcc catggtaaag
    10681 gatgggcgaa taatgcacgg gaagccaact tcagcggcaa ccgccagcgc ttcttccatt
    10741 gaatgagcga taccggaacg cgcggtttcc agaccgattt tcttcatcgc tacgtcgaaa
    10801 cggcggcggt cttcggcttt atcaatcgca tcggcagtgg caccaatcat ggtgacgccg
    10861 aactcttcca gcacgccctg acgctcaagc tcaagcgcac agttcagcgc cgtctgcccc
    10921 cccatggttg gcagcaccgc gtccggacgc tctttttcaa taatcttgcg taccacttcc
    10981 cagtgaatcg gttcgatgta ggttgcatca gccatttccg ggtcggtcat gatggtcgcc
    11041 gggttggagt tcaccagaat gacgcggtaa ccctcttcac gcagggcttt acacgcttgc
    11101 gcgccagagt agtcaaactc acacgcctga ccgataacaa tcgggcccgc acccagaatc
    11161 aggatacttt ttatatctgt acgttttggc atggctcttt tactcctgat tacttagcgg
    11221 ttttacggta ctgctcaatt aactcgataa agtggtcaaa caacggcgcg gcgtcgtgtg
    11281 gaccagggct ggcttccggg tgtccctgga agctgaacgc cggtttatcg gtacgatgaa
    11341 tgccctgtaa cgtaccgtcg aacagggatt tatgtgtgac acgcaggttt gcaggtaatg
    11401 ttgcttcatc caccgcaaaa ccgtggttct gggcggtaat catcaccaca tttttctcaa
    11461 catctttaac cggatggttg ccgccgtggt gaccaaattt catttttaca gtcttcgcac
    11521 cgctcgccag cgccagcagc tgatgaccga ggcagatgcc gaataccgga atatcggttt
    11581 cgaggaattt ctggatggcg gtaatggcgt aatcgcacgg ggccgggtcg ccaggaccgt
    11641 tggagaggaa gatgccgtct ggattcattt tcagcacgtc ttccgcagaa gtttgcgccg
    11701 gaacgatggt cagacgacag cctctatcca ccaacatgcg caggatgttg cgcttggcac
    11761 caaaatcata agccacaaca tggaacggca gctcgtcttc tttcttcgct tctggcaggc
    11821 cgccggtcaa cgtccagctc ccttgtgtcc agctataggc ttctgcggtg gtcacttctt
    11881 ttgccagatc catgccgttc agacctggga acgcgcgggc tttttctaat gccagcgccg
    11941 catccgggtt atcgcctgcg ataatgcagc cattctgtgc gcctttctcg cgcagtaaac
    12001 gcgtcagctt acgggtatcg atatcggcaa tcgccacgat gttatggcgc ttcaggtaag
    12061 aagagaggtc ttcggtatta cggaagttgc tggcaatcag cggcaggtcg cgaatcacca
    12121 gaccttgtgc atgtacctga gaagattctt catcggcgtc attagtgccg acattaccaa
    12181 tatggggata agtaagagta acgatttgac gagaatagga aggatcagtg aggatttctt
    12241 gataaccggt cattgaagta ttgaaaacga cttccccaac cgccgaacct gttgccccta
    12301 tggcccgacc gtgaaactgg gttccgtctt ccagaaccaa tagcgctgac ttaatcaaaa
    12361 caccctccag agaatattca ctcactttat ttgcatatta attcacaatg atgacgtaaa
    12421 tcaatgcaaa tctgcttacc gacgaatttc tggcaaacgg cggcattctg gagatatggg
    12481 cgctaaaagt caacttaatg gcgggatttt ttatattatt ttgtggctca tgcatgtttt
    12541 acaggcatta agcgacaaaa agatcatata acatgttctg gaaaacgctt gcgcagcaag
    12601 aagattgcat gaaatcaaaa tgagcagaaa aaagtggacc aaatggtcaa aatttacatt
    12661 aaaacaacaa aaacagaa
//
LOCUS       DAZFLD010000138        12636 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.139, whole genome shotgun sequence.
ACCESSION   DAZFLD010000138 DAZFLD010000000
VERSION     DAZFLD010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12636
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.139"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(57..947)
                     /locus_tag="ACHKBL_003370"
     CDS             complement(57..947)
                     /locus_tag="ACHKBL_003370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000236948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper family protein"
                     /protein_id="HFO5820197.1"
                     /translation="MVISENTVGTNDIPDSNHDILGPLMPARGFFFIVIDEPGKDGFL
                     VRKIYASPNSPYLTKMNLDPLRLLSETEPERYGLMPKDYSAPTTVAEHVMGNNKSSLI
                     STSSIFPDGSPRFEGKTIFIDVHKAKNAGLKLISTAEIITALEEYKKQYPHLAKRVEK
                     ISGYVDDIDKEVLIEGRKVPAQTIFTPESLKTTQNIVKAGRVVQVFSIFFTAYDLEQA
                     TEKSFETKSIKPIGAEAIRQIGGWGGAVAGAKIGTVIGASLGVTTGPGAVATGIIGGI
                     VFGAAGYFGADWVADYIDEN"
     gene            complement(941..3430)
                     /locus_tag="ACHKBL_003371"
     CDS             complement(941..3430)
                     /locus_tag="ACHKBL_003371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001298160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system Vgr family protein"
                     /protein_id="HFO5820198.1"
                     /translation="MGIQNFDHSHHKLKIRGLKSPVDVLTFTGREQLSAPFRYDIEFT
                     STDKTIEPESVLMQDGAFSLTAPPVQGMPVQTALRTLHGVITGFKHLSSSQDEARYEV
                     RLEPRMALLTRSRQNAIYQNQTVPQIVEKILRERHQMRGQDFVFNLKSEYPSREQVMQ
                     YGEDDLTFISRLLSEVGIWFRFATDARLKIEVIEFYDDQSGYERGLTLPLRHPSGLFD
                     GETEAVWGLNTAYSVVEKSVSTRDYNYREATAEMTTGQHDATGGDNTTYGEAYHYADN
                     FLQQGDKEAAESGAFYARIRHERYLNEQAILKGQSTSSLLMPGLEIKVQGDDAPAVFR
                     KGVLITGVTTSAARDRSYELTFTAIPYSERYGYRPALIPRPVMAGTLPARVTSTVKND
                     IYAHIDKDGRYRVNLDFDRDTWKPGYESLWVRQSRPYAGDTYGLHLPLLAGTEVSIAF
                     EEGNPDRPYIAGVKHDSAHTDHVTIQNYKRNVLRTPANNKIRLDDERGKEHIKVSTEY
                     GGKSQLNLGHLVDAGKQQRGEGFELRTDMWGVVRAKKGIFISADAQDNAQGQVREMAP
                     AMAILNGAQSQMKSLSTDAQTANADPADLSSQIALLQQSVKDLNQAAILLSAPKGVAI
                     ASGEHLQLAAAKNVIANAGNNADVGAVKNMFIGVGQALSVFVRKAGIKLFANKGAISV
                     QAQNDLMELLAQKSIEITSTEDEIKITAKKKITLNGGGSYIRLDACGIEAGTQGEYNV
                     KAGYYGRKPKAKLTPELMAFPVISAKFDEQVRVIDDDGKFIANVPYYIEDSIGNIYKG
                     FTDSAGCCERIFTEEADNLNVLLGIKALEKW"
     gene            complement(3442..6087)
                     /gene="tssH"
                     /locus_tag="ACHKBL_003372"
                     /gene_synonym="clpV"
     CDS             complement(3442..6087)
                     /gene="tssH"
                     /locus_tag="ACHKBL_003372"
                     /gene_synonym="clpV"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001535533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ATPase TssH"
                     /protein_id="HFO5820199.1"
                     /translation="MTGNHPAALLRRLNPYCARALDAAASLCQTRAHAEITIEHWLLK
                     LLEQGEGDITVIARRYEWDIDTLWQSLLAHLDTLPRSVRERPQLSEPLAALIRQAWLI
                     ASLEGDDPQIRSQHLLMALTEKPMLPACNDLWVLLSLSRVQLERLHPLLDAQSDECPA
                     RQPQVTEPLTSAQPESATTDVPAKTLTGKQDDALLAVLNRFTEDVTEKARSGRIDPVF
                     GRDTEIRQMVDILSRRRKNNPILVGEPGVGKTALVEGLALRIAEGNVPDSLKTVHIRT
                     LDLGLLQAGAGVKGEFEQRLKNVIDAAQKSPEPVLLFIDEAHTIIGAGNQAGGADAAN
                     LLKPALARGELRTIAATTWSEYKQYFERDAALERRFQMVKVDEPDDDTACLMLRGLKA
                     RYAQHHGVHMLDSAIQTAVRLSRRYLTGRQLPDKAVDLLDTAGARVRMSLDTLPEPLT
                     QLHARLAALDIEREAIEQDSVFYPETSPERLAELTDLRDELQAEAGHLEAQYQQEKAL
                     VQQIMTLRQEGTDSSELQQQLRTHQGFAPLLALDVDARAVATVVADWTGIPLSSLLKD
                     EQSDLLSMEQSLENRVVGQRPALCAIAQRLRAAKTGLTPENGPQGVFLLTGPSGTGKT
                     ETALTLADTLFGGEKSLITINLSEYQEPHTVSQLKGSPPGYVGYGQGGVLTEAVRKRP
                     YSVVLLDEVEKAHRDVMNLFYQVFDRGFMRDGEGREIDFRNTVILMTANLGSDHIMQL
                     LEEKPDATDADLHELLYPLLREHFQPALMARFQTVIYRPLGQEAMRTIVEMKLAQVVR
                     RLHQHYGLETEINDSLYDALTAACLLPDTGARNIDSLLNQQILPVLSQQLLAQQAAHR
                     KPAQLRLGWDEEDGIVLEFATEEMQ"
     gene            complement(6279..6770)
                     /locus_tag="ACHKBL_003373"
     CDS             complement(6279..6770)
                     /locus_tag="ACHKBL_003373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
                     /protein_id="HFO5820200.1"
                     /translation="MAIPAYLWLKDDGGADIKGSVDVQGREGSIEVVALDHDVYIPTD
                     NNTGKLTGTRTHKPFTFTKEIDASSPYLYKAVPTGQTLKTAEFKFYRINDAGQEVEYF
                     NITLDNVKLVRVAPLMHDIKDPSREKHNHLERIEFRYEKITWTYKDGNIIHSDSWNER
                     PSA"
     gene            complement(6828..8480)
                     /locus_tag="ACHKBL_003374"
     CDS             complement(6828..8480)
                     /locus_tag="ACHKBL_003374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001246525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HFO5820201.1"
                     /translation="MRNTLKQAIVLWGMVLLLVLWWVFVSPSGVLRWAGAAVIVLAVA
                     ALLIYRRRQTWTEMTGDAGLLSLPPETYRQPVVLVCGDMSAHLFTDSPVRQVSEGLYL
                     HVPDEEQLVAQVERLLTLRPAWASQLAVAYTVMPGMYRDTAVLTGQLRRFAHSMAIVR
                     RRAGVNVPWLLWSGLSGSPLPERANSPWFICTGGEVQVATSAETTMPAQWIAQSGAQE
                     RSQRLSYLLKAESLMQWLDLNVLAELNGPEAKCPPLAMAVGLVPSLPAVDNNLWQLWI
                     TARTGLTPDIADTGTDDALPFPDALLRRLPRQSGFTPLRRACVTMLGVTTVAGIAALC
                     LSATANRQLLRQVGDDLHRFYAVPVEEFITKARHLSVLKDDATMLDGYYREGEPLRLG
                     LGLYPGERIRQPVLRTIRDWRPPEQKMEVTASLQVQTVRLDSMSLFDVGQARLKDGST
                     KVLVDALVNIRAKPGWLILVAGYTDATGDEKSNQQLSLRRAEAVRNWMLQTSDIPATC
                     FAVQGLGESQPAATNDTPLGRAVNRRVEISLVPRSDACQDVK"
     gene            complement(8493..9158)
                     /gene="tssL"
                     /locus_tag="ACHKBL_003375"
     CDS             complement(8493..9158)
                     /gene="tssL"
                     /locus_tag="ACHKBL_003375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssL, short
                     form"
                     /protein_id="HFO5820202.1"
                     /translation="MKKETDINIDVLLRDTFLTVVELRQGTTVRHGIELYRHCQRQVE
                     LVRERLKDAGFSRENVEHITYAQCALLDETVLSRSGMDDGQAIWMKNPLQSHFFNTLQ
                     AGELLYERMKQVLQEPAPAQAVLTCFHRVLLLGFRGRYQDPVAPERDQLISTLNGLVA
                     PFGVLPETAVLNVPLSPRQHPLLHSPFFWLVTLTLLLAGVWWGLHHWLNVLVDELLPQ
                     SLR"
     gene            complement(9155..10492)
                     /gene="tssK"
                     /locus_tag="ACHKBL_003376"
     CDS             complement(9155..10492)
                     /gene="tssK"
                     /locus_tag="ACHKBL_003376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
                     /protein_id="HFO5820203.1"
                     /translation="MKICRPLWAEGTFLSSQQFQQQARWEAFSNDSIAQLCIRHPWGI
                     SNVLFDRDALTLGKLKTQAVRLRFADGTLIDSDVADILPPACDLRTLEGGSAIVLLAL
                     PLAHGNGGNLGQGEQAERPLRYRQEWQKVQDIYGSDSEDMAVERHALSLRFAHDNNQD
                     YITCPLARLVRDVQGNWTQDESYIPPLLTLNAHDGLVQRLDTLLLQLRAKCQRLMAMR
                     RESNQRMADFAVADVSLFWLLNALNSAEPVLSDFLRYPAVHPELVWRELARLAGALLT
                     FSLEHNVSAVPPYVHESPSTVFPPLFSLLSELLEASLPSRVIALDLASLPGNRWKADL
                     HDPRLQEEADFYLSVRSSLPAHQVLHQLPLVCKIGAPDDVTLLINVALNGVPLVPLTS
                     VPAALPLRLENQYFALDMHSDAAKSMLESGCCMIYAPGTMGDLKPELFAVLRS"
     gene            complement(10512..12050)
                     /gene="tssC"
                     /locus_tag="ACHKBL_003377"
     CDS             complement(10512..12050)
                     /gene="tssC"
                     /locus_tag="ACHKBL_003377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
                     /protein_id="HFO5820204.1"
                     /translation="MSVQQEYATSETATLTTTESGGVYQSLFDKINLTPVSSIQEIDL
                     WQNSETLADASPDERVTAAIHVLLSCLAKSGEDVVKLDKSLLDFHIDDLDQKISKQLD
                     AVMHHPEFQKVESLWRGTWFVVQRTDFRKNVKIELLDISKEHLRQDFDDSPEIIQSGL
                     YRHTYIQEYDTPGGEPVASLISSYEFDNSPQDIALLRNISRVSAASHMPFIGSVGPKF
                     FLKNSMEEVAAIKDIGNYFDRAEYIKWKSFRDTDDSRYVGLVMPRVLGRLPYGPDTVP
                     VRSFNYVEEVKGPDHEKYLWTNASFAFAANMVKSFVNNGWCVQIRGPQAGGAVADLPI
                     HLYDLGTGNQVKIPSEVMIPETREFEFANLGFIPLSYYKNRDYACFFSANSAQKPALY
                     DTADATANSRINARLPYIFLLSRIAHYLKIIQRENIGTTKDRRVLELELNTWIRTLVT
                     EMTDPGDELQASHPLRDGKVIVEDIEDNPGFFRVRLFAVPHFQIEGMDVNLSLVSQMP
                     KAKA"
     gene            complement(12109..12609)
                     /gene="tssB"
                     /locus_tag="ACHKBL_003378"
     CDS             complement(12109..12609)
                     /gene="tssB"
                     /locus_tag="ACHKBL_003378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     small subunit"
                     /protein_id="HFO5820205.1"
                     /translation="MADSFQNEVPAARVNIKLDLHTGNAKKKVELPLKLLAVGDYSNG
                     KEQRPLSERDKVDINKNNFNSVMAEFSPAVNLTVEDTLNGNGNEQNIALEFKSLKDFE
                     PEQVAKNIPQLRVLLAMRNLLRDLKSNLLDNATFRRELENILKDPTLSSELRDELAKI
                     APQENV"
BASE COUNT         2616 a         3632 c         3384 g         3004 t
ORIGIN      
        1 aaaaatcttt aacttcaggt tttttaacga gcaaattctt catgtgctct atccaattag
       61 ttttcatcta tataatccgc tacccaatct gcaccaaaat aaccagctgc accaaaaaca
      121 atcccgccaa taatcccggt agccactgct cctggcccag tagtaacacc cagggacgcg
      181 cctatcactg ttccaatttt agcaccagct actgctcctc cccatcctcc aatttgcctt
      241 attgcttctg cacctatagg ttttatgctt tttgtttcaa acgatttttc agttgcctgt
      301 tctaaatcat atgcagtgaa aaaaatacta aaaacttgta cgacacgccc ggccttaaca
      361 atgttttgag tcgtttttag agactctggt gtaaaaattg tttgtgctgg tacttttcta
      421 ccttcgatca agacctcttt atcaatatca tcaacatagc ctgaaatttt ttcaacacgc
      481 ttagccaaat gagggtattg tttcttatac tcctcaagag cagtaataat ctctgcggtc
      541 gagattaact tcaacccggc attttttgct ttgtgaacat caataaatat ggttttcccc
      601 tcaaatctcg gagagccatc aggaaatata gagcttgtag aaatcaacga ggatttgttg
      661 ttccccataa catgttctgc tacagtagtg ggtgctgaat aatcttttgg catcaaccca
      721 tagcgttcag gctctgtttc tgataataat ctaagaggat ccagattcat ctttgtcagg
      781 taggggctat ttggtgaagc atatattttt ctgacaagaa agccatcttt tcccggctca
      841 tcgataacta taaaaaagaa acctcttgca ggcattaagg gtcctaatat atcgtgattt
      901 gaatcaggta tatcatttgt tcctactgta ttctcagata tcaccatttt tccagcgcct
      961 taataccaag aagtacgttt aggttatcag cttcttccgt gaaaattctt tcacagcacc
     1021 cggcagaatc agtaaacccc ttatagatat tccctatgct gtcttctata tagtaaggta
     1081 cattagcaat gaattttccg tcatcatcaa ttactctaac ttgctcatca aatttcgcag
     1141 atattacagg aaacgccatc agctccggcg tcagcttcgc ctttggttta cgcccgtaat
     1201 atccggcctt aacgttatat tctccctgcg ttcccgcttc tatcccacag gcatccagcc
     1261 ggatgtagct gccaccgccg ttaagggtga ttttcttctt cgcggtgatc ttgatctcat
     1321 cttccgtact ggtgatctct atcgatttct gtgccaacag ttccatcaga tcgttctgtg
     1381 cctgtaccga gatcgccccc ttgttggcaa acagcttgat gcccgcttta cgcacaaaca
     1441 cgcttagcgc ctgtcccacg ccgataaaca tatttttcac cgcgccgaca tcggcgttgt
     1501 tgcctgcgtt ggctatcaca ttctttgccg ccgccagttg caggtgctcg ccgctggcga
     1561 tcgccacgcc cttgggtgcg cttagcagga tcgcagcctg gttcagatct tttacgctct
     1621 gttgcaacaa cgcaatctgc gatgacagat ctgccggatc ggcattcgcc gtctgcgcat
     1681 cagtggacag agacttcatc tgtgactgtg cgccattcag tatcgccatt gcaggagcca
     1741 tttcgcgcac ctgcccctgc gcattatcct gcgcatcggc gctgataaag atgcctttct
     1801 ttgcccgcac cacgccccac atatcggtac gcagttcaaa gccttcgccg cgttgctgtt
     1861 tcccggcatc caccagatga ccgaggttta gctggctctt gccgccgtac tcggtactga
     1921 ccttgatatg ttctttgccg cgctcatcgt cgaggcggat tttgttgttc gccggggttc
     1981 tgaggacgtt acgcttatag ttctgaatgg tcacatggtc agtgtgtgcc gagtcgtgtt
     2041 ttacgcctgc aatataaggt cggtccgggt tgccttcctc aaaggcgata ctgacttccg
     2101 ttcccgccag cagcggcagg tgcaggccgt aggtgtctcc cgcatacggg cgcgactggc
     2161 gtacccacag gctctcatag cccggtttcc acgtatcgcg gtcaaagtcg aggttgaccc
     2221 ggtagcgccc gtccttgtcg atatgggcgt agatgtcgtt cttcaccgta ctggtgaccc
     2281 gcgccgggag cgttccggcc attacggggc gggggataag cgccggacgg tagccgtagc
     2341 gctctgagta cggtatcgcc gtgaacgtca gctcatagct gcggtcacgc gccgcgctgg
     2401 tcgtcacgcc ggtaatcagc acgcccttgc ggaacaccgc aggcgcgtcg tcgccctgaa
     2461 cttttatctc cagccccggc atcagcagtg aactggtgct ctggcctttg agaatggcct
     2521 gttcattcag gtagcgttcg tgacggatac gggcataaaa cgcgccgctt tccgctgcct
     2581 ctttatcgcc ttgttgcagg aagttgtcgg catagtggta ggcttcgccg taagtggtgt
     2641 tatcgccccc ggtggcgtca tgctggcctg tggtcatttc cgccgtggct tcacggtagt
     2701 tgtagtcccg cgtggagaca cttttttcca ccacgctgta cgcggtgttc agcccccata
     2761 cggcttccgt ttcaccatca aacagcccag aaggatggcg aagcggcagc gtcagcccgc
     2821 gctcgtagcc gctctggtcg tcgtaaaatt cgataacttc gattttcaac cgggcgtcgg
     2881 tggcaaagcg gaaccagata cccacttctg acagcaggcg tgaaataaac gtcaggtcgt
     2941 cctcaccgta ctgcatcacc tgttcacggg acgggtattc gcttttgagg ttaaacacga
     3001 agtcctgacc gcgcatctgg tgccgctcgc gcaggatttt ttcgacgatt tgcggcaccg
     3061 tctggttctg gtagatggcg ttctggcggc tgcgggtaag cagcgccata cggggttcaa
     3121 ggcgcacttc gtaacgggct tcgtcctgtg acgatgagag atgtttaaag ccggtaatga
     3181 cgccgtgcag ggtgcgcagg gcagtctgta ccggcattcc ctgtaccggc ggtgccgtca
     3241 ggctgaatgc gccatcctgc atcagcaccg actccggctc gatggtttta tcggtactgg
     3301 taaattcaat gtcgtagcgg aagggtgcgc tgagttgttc ccggcccgta aacgtcagca
     3361 cgtccaccgg gcttttcagg ccgcgaattt tgagcttgtg gtgtgagtgg tcgaaattct
     3421 gaatacccat aaaagcctcc gttattgcat ctcttccgta gcgaactcca gtacaatccc
     3481 gtcttcctca tcccagccaa gccgtagttg ggctggttta cggtgcgcgg cctgctgtgc
     3541 cagcaactgc tgacttagta ccggcaggat ttgctggttc aacaggctgt cgatattgcg
     3601 tgcaccggtg tccggcagca ggcaggcggc ggtcagcgcg tcatacaggc tgtcgttgat
     3661 ttccgtttcc agcccatagt gctgatgaag acgacgaacc acctgcgcca gtttcatttc
     3721 cacaatggtg cgcatcgcct cctgtcccag cgggcggtaa atcaccgtct gaaaacgcgc
     3781 catcagtgca ggctggaagt gctcgcgcag caggggatac agcagttcat gcaggtctgc
     3841 gtccgtggcg tccggttttt cctccagcag ttgcatgatg tggtcgctgc ccagattagc
     3901 cgtcatcaga atcacggtgt tgcggaagtc aatttcccgg ccttcgccgt cgcgcataaa
     3961 accccggtca aatacctgat agaacaggtt cattacgtcg cggtgcgcct tttccacttc
     4021 gtcgagcaac accacgctgt acgggcgttt gcgaacggct tcggtaagta cgccgccctg
     4081 accgtagcca acgtagcccg gaggagaccc cttaagctgg gagacggtat gcggctcctg
     4141 atattcggag aggttgatgg tgataagaga tttttcaccg ccaaacaggg tgtcggccag
     4201 tgtgagcgca gtttcggttt taccggtgcc gctggggccg gtcagcagga ataccccctg
     4261 tgggccgttc tccggcgtga ggccggtttt agccgcccgc aaccgctgtg cgatggcgca
     4321 gagcgccggg cgttgcccga caacgcggtt ttcaaggctc tgttccatac tgagcaggtc
     4381 gctctgctcg tccttgagca gggaagagag cgggatgcca gtccagtccg ccaccaccgt
     4441 ggcgacggcg cgggcgtcca catccagcgc cagcagcggt gcaaagccct gatgcgtccg
     4501 cagttgctgt tgcagttcag agctgtctgt tccttcctga cgcaacgtca taatctgctg
     4561 caccagtgcc ttttcctgct gatactgcgc ttccagatgc ccggcttctg cctgcagctc
     4621 atcacgcaaa tcggtcagtt ctgccagccg ctccgggctg gtttcgggat aaaacacgct
     4681 gtcctgctca atcgcctccc gctcgatatc cagcgccgcc agtcgcgcat gaagctgcgt
     4741 cagcggctcc ggcagggtgt cgaggctcat gcggactctt gctccggcgg tatccagcaa
     4801 atcaaccgcc ttgtccggca gctggcgtcc ggtcagatag cggcgcgaca ggcgcacggc
     4861 ggtctgaatg gcgctgtcca gcatatgcac gccgtggtgc tgtgcataac gggccttcag
     4921 tccccgcagc atcagacagg cggtgtcatc atccggctcg tcaaccttaa ccatctggaa
     4981 gcggcgctcc agcgcggcgt cgcgctcaaa atactgtttg tattcgctcc acgtggtcgc
     5041 cgcgatggtg cgcagttcgc cccttgccag tgccggtttc agcaggttcg ccgcatccgc
     5101 gccgccagcc tggttacccg cgccgataat ggtatgggct tcatcaataa acagcagtac
     5161 cggctccggc gatttctgtg ctgcatcaat cacgtttttc agtcgctgct caaattcacc
     5221 tttaacaccc gcgccagcct gtaacagacc gaggtccagc gtgcggatat gcaccgtttt
     5281 caggctgtcg ggcacgttgc cttcggcgat acgcagcgcc agcccttcca ccagtgcggt
     5341 tttgcccacc cccggttctc ccaccagaat cgggttgttt ttgcggcgac gggagaggat
     5401 atcgaccatc tggcgaattt cggtgtcgcg cccgaatact gggtcgattc gcccgctacg
     5461 ggctttttcc gtcacgtctt cggtaaagcg gttaagcacc gccagcaggg cgtcatcctg
     5521 tttccccgtc agcgtttttg ccggtacgtc cgtcgttgcc gactctggtt gcgcggatgt
     5581 cagcggttcg gtgacctgtg gctgacgtgc cggacattca tccgactgcg catccagcag
     5641 gggatgcagc cgctcaagct gcacgcggct cagactcagc aatacccaca ggtcattaca
     5701 ggcgggcagc atcggttttt ctgtcagcgc catcagcagg tgctggctgc ggatttgtgg
     5761 atcgtcacct tccagtgacg ctatcagcca cgcctgtctg ataagcgctg ccagcggttc
     5821 agaaagttga ggacgttcgc ggaccgagcg gggtaaggtg tccagatgcg ccagcagaga
     5881 ctgccagagc gtgtcgatat cccattcata gcgacgggca atcaccgtga tatcgccttc
     5941 tccctgctcc agcagtttca gcagccagtg ttcaatggtt atctccgcat gggcgcgggt
     6001 ctgacacagt gaggcggcag cgtccaacgc ccgtgcacag tatgggttaa ggcgacgcag
     6061 cagcgcggcg gggtgatttc ctgtcataga agggtttcct tatcgtgttc tgtattcctg
     6121 cgatccgcgt tcaggcacgc taccgcccat agccgttacc tcacacaggc ccatcaggtt
     6181 tcaccagatt gtgggtatgc tttgctcagt accggtgcct gcggcactgg acaaagcaca
     6241 caacaaaaag gcagacagtt tcctgtctgc cgcagttatc aggcggaagg acgctcattc
     6301 cacgagtcgg aatgaatgat gttgccgtct ttgtaagtcc aggtgatttt ctcgtagcgg
     6361 aactcaatac gttccaggtg gttatgcttc tctctggaag gatccttgat gtcgtgcatc
     6421 agcggagcga ctctgaccag cttgacgtta tcaagcgtga tgttgaagta ctccacttcc
     6481 tgaccggcat cgttgatgcg gtaaaactta aattctgccg ttttcagggt ctgaccggtg
     6541 ggcacagctt tgtagagata cggactggac gcatcgattt ctttggtaaa cgtaaagggc
     6601 ttgtgagtac gggtaccggt cagtttgccg gtgttgttgt cggtcgggat gtacacatcg
     6661 tgatccaacg ccaccacttc gatgctgcct tcgcgcccct gaacgtccac ggaacctttg
     6721 atatctgcgc cgccgtcatc tttcagccag agataagcag gaatagccat gagaattact
     6781 ctccttgtta gtgtgattga agcaccgcct tcttccggcg gtatgtttta tttcacgtcc
     6841 tgacaggcgt cagaacgcgg aacaagactg atttcgacgc gccggttgac tgccctgccc
     6901 agtggcgtat cgttggtcgc cgcaggctgg ctctcaccca gtccctgtac ggcaaaacag
     6961 gtggccggga tgtcgctggt ctgcagcatc cagttacgca ccgcttcggc acgtcgcagc
     7021 gataactgct gattgctttt ttcgtcgccg gtggcgtcgg tatatccggc cacgaggatc
     7081 agccagcccg gttttgcccg gatgttcacc agtgcgtcca ccagcacttt tgtcgagccg
     7141 tctttcaggc gggcctgtcc gacgtcaaac agcgacatac tgtcaagacg cacggtctga
     7201 acctgaagcg aagccgtcac ctccattttt tgttcaggcg gacgccagtc gcgaatggtg
     7261 cgtaataccg gctggcggat gcgttcgccg gggtataacc ccagaccgag gcgcagtggt
     7321 tctccttccc ggtaataccc atcgagcatg gtcgcatcgt ctttcagcac cgacaggtga
     7381 cgggctttgg tgataaattc ctccaccggg acggcataaa accggtgcag atcgtcaccg
     7441 acctgccgta ataactggcg atttgccgtg gctgacaggc acagcgcggc gatacccgcc
     7501 acggtggtga cgcccagcat ggtcacgcag gcccgtcgca gcggggtaaa gcccgactga
     7561 cgcggcaacc gccgtaacag ggcatccggg aatggcagcg catcgtctgt gccggtgtcc
     7621 gcgatatccg gcgtcaggcc ggttctggcg gtgatccaca actgccatag gttgttatcc
     7681 accgcaggca acgaggggac cagccccacg gccatcgcca gtggtggaca tttcgcctcc
     7741 gggccgttca gttccgccag cacattcaga tccagccatt gcatcaggct ttcggctttc
     7801 agcagataag agagtcgctg actgcgttcc tgtgcgccgg attgtgcaat ccactgcgcg
     7861 ggcatggtgg tctctgcgga tgttgctacc tgaacttcgc cgccggtaca gataaaccac
     7921 ggtgaattcg ctctctccgg caacggcgag ccggacagcc cgctccagag aagccagggt
     7981 acgtttacgc ccgcccggcg acgcactatc gccatactgt gagcgaaccg tcgcagttgt
     8041 ccggtcagaa ccgccgtatc ccggtacatg ccgggcatga cggtatacgc cacggcaagc
     8101 tgcgatgccc acgccgggcg aagggtcagt aatcgctcca cctgcgccac aagctgttct
     8161 tcatcgggaa catgcagata cagcccttct gaaacctgcc ggaccgggct gtcagtgaac
     8221 agatgcgctg acatatcgcc acagaccagc actaccggtt gtcggtacgt ttccggcggc
     8281 agcgataaca acccggcatc gccggtcatc tccgtccacg tctgcctgcg ccggtaaatc
     8341 aacaatgcgg caaccgccag aacgataact gctgcaccgg cccatctcag tacgccagac
     8401 ggactgacaa acacccacca cagcaccagc agtaacacca ttccccacag cacgatggcc
     8461 tgtttcagcg tgttcctcac tcacggctcc ctttaccgca ggctttgcgg cagcaattca
     8521 tccaccagca cgttcagcca gtggtgcagt ccccaccaga caccggccag tagcagcgtc
     8581 agcgttacca gccagaaaaa cggcgaatgc agcaaaggat gctgacgggg ggataacggc
     8641 acgttcagta ccgccgtttc cggcagcacg ccaaatggcg cgaccagccc gttgagcgtc
     8701 gaaataagct ggtcacgctc cggggccacc ggatcctgat aacgcccgcg aaatcccagc
     8761 agcaggacac ggtgaaagca ggtcagcact gcctgcgcgg gggcgggctc ctgcaacacc
     8821 tgcttcatcc gttcgtacag cagctcgcct gcctgcagag tgttgaagaa atgcgactgg
     8881 agtgggtttt tcatccagat ggcctgaccg tcgtccatgc cactacggct gagcaccgtt
     8941 tcgtccagca gggcgcactg cgcgtaagtt atgtgctcca cgttctcccg actgaatccg
     9001 gcatctttca gacgttcgcg gaccagttcc acctgccgct gacagtggcg atacagctcg
     9061 ataccgtgac gcacggtggt tccctgacgc agttccacca ccgtcagaaa cgtgtcgcgc
     9121 agcagcacgt cgatattgat atccgtctct tttttcatga gcgcagcacc gcaaacagtt
     9181 caggtttcag gtcgcccatt gttcccggcg cataaatcat gcagcacccg gactccagca
     9241 tcgatttagc cgcgtcactg tgcatatcca gcgcaaaata ctggttctcc agccgcagcg
     9301 gcagcgccgc cggaaccgag gtgagtggca ccagtggaac accgttcagc gccacgttga
     9361 taagcagcgt gacgtcatca ggcgcaccga ttttgcagac cagcggtagc tggtgcagta
     9421 cctgatgcgc aggcagtgag gaacgaacgg acaggtaaaa atcggcttct tcctgcaggc
     9481 gcggatcgtg caggtcggct ttccagcggt tgcccggcag ggacgccaga tccagcgcga
     9541 tcacccgcga cggcaggctg gcttccagca gctcgctgag cagggagaac agtggcggaa
     9601 agacggtgga cggcgattcg tggacatagg gcggaacggc gctgacgtta tgctcaaggg
     9661 aaaacgtcag cagcgcaccg gcaagcctcg ccagctcccg ccagaccagt tcggggtgga
     9721 cagccgggta gcgcaggaaa tcgctcagca ccggctcggc ggaattcagg gcgttaagca
     9781 gccagaacag agagacatcg gcgacggcga agtccgccat gcgctggttg ctttcccggc
     9841 gcatcgccat caggcgctgg cacttcgccc gtagctgcaa cagcagcgtg tccaggcgct
     9901 gtaccagccc gtcatgggcg tttaacgtca gtaacggcgg aatatagctc tcatcctgcg
     9961 tccagttgcc ctgaacatcc cgcaccagac gcgccagcgg acaggtgatg taatcctgat
    10021 tgttatcatg ggcaaagcgc agggacagtg catggcgctc aacggccata tcttcactgt
    10081 cactgccgta gatatcctgc accttttgcc attcctgacg atagcgcagc ggcctttcag
    10141 cctgctcgcc ctgaccgaga ttaccgccgt taccatgcgc aagcggcagc gccagcaaaa
    10201 caatcgcgct gccgccctcc agcgtgcgca ggtcacaggc aggcggcaga atatccgcca
    10261 cgtctgaatc gatcagcgta ccatcagcaa aacgcaggcg tactgcctgc gttttcagct
    10321 ttccgagcgt cagggcatcc cggtcaaaga gcacgtttga gatcccccac ggatggcgaa
    10381 tacaaagctg cgcaatgctg tcattgctga acgcttccca gcgggcctgc tgctgaaact
    10441 gctgagaaga caggaatgtc ccctcagccc acagtggtcg acaaattttc atacatcaca
    10501 ccaccgcccg atcaggcttt tgcttttggc atctgggaaa ccagagaaag gttgacgtcc
    10561 atcccttcga tctggaaatg cggcacggca aagagtctga cgcggaaaaa gcccggattg
    10621 tcctctatgt cctcgacgat aactttcccg tcgcgcagtg gatgcgaagc ctgaagttca
    10681 tcgcccggat cggtcatttc cgtcaccagc gtgcggatcc aggtgttcag ctccagttcc
    10741 agcacgcgtc tgtctttggt ggtgccgata ttctcgcgct gaatgatttt caggtagtga
    10801 gcaatgcggg acagcaggaa gatatagggc aaacgggcgt tgatacggct gttggcggtg
    10861 gcgtcagcgg tatcatacag cgccggtttc tgggcagagt tcgctgagaa gaagcaggcg
    10921 taatcgcgat tcttataata agagagcggg ataaagccaa ggttggcgaa ctcaaactcg
    10981 cgggtttccg ggatcatcac ttcggacgga attttgacct gattgccggt gccgagatcg
    11041 taaagatgga tcggcagatc ggctactgcg ccacccgcct gcggaccacg gatctgcaca
    11101 caccagccat tattcacaaa actcttcacc atattggcgg caaaggcaaa cgaggcgttt
    11161 gtccagaggt acttttcgtg atccgggcct ttgacctctt ccacatagtt aaagctgcgt
    11221 accggcaccg tgtccgggcc atagggcaga cggcccagca cgcgcggcat cactaatccc
    11281 acgtagcggc tgtcgtcggt atcgcggaac gacttccatt tgatatattc cgcgcggtca
    11341 aaatagttgc cgatatcttt aattgcggcg acttcttcca tcgagttttt aaggaagaac
    11401 ttcggtccga cggaaccgat aaagggcata tgtgatgcag cagagactct ggaaatatta
    11461 cgcagcaggg caatatcctg cgggctgtta tcgaattcat agctggatat taatgaggct
    11521 acaggttcgc cacccggcgt atcatattcc tgaatgtatg tatggcgata taaaccactt
    11581 tgaatgattt ccggagaatc atcaaagtcc tgccgcagat gttctttact gatatccagc
    11641 agttcaattt tgacattttt gcgaaaatcg gtgcgctgta cgacgaacca tgtgccgcgc
    11701 cagagcgact cgactttctg gaattccgga tggtgcatga ctgcatcaag ctgtttactg
    11761 attttctgat ccaaatcgtc gatatgaaaa tccagcaggc tcttgtcgag tttaaccacg
    11821 tcctcgcctg atttcgccag acaggaaagc agaacgtgga tcgccgccgt cacgcgctca
    11881 tcgggtgaag catcggccag cgtttcgctg ttttgccata aatcgatttc ctgaatggaa
    11941 gagaccgggg ttaaattaat tttatcaaac agggactgat aaacgccgcc ggactcagtg
    12001 gtggtgagtg ttgcagtttc agaggtggca tattcttgtt gtacagacat cagcatttcc
    12061 tttttaggga tcctttcttc tccggtttaa cctgaatacg cgttcgggtt aaacgttctc
    12121 ctgcggcgca attttcgcca gttcatcacg cagttctgaa ctgagcgtcg ggtctttcag
    12181 gatattttcc agttcacggc gaaacgttgc gttatccagc agattggatt tcaggtcacg
    12241 tagtaaattg cgcatagcga gtaatacacg aagttgcgga atattttttg ctacctgttc
    12301 gggttcaaaa tccttcaggc ttttaaattc aagcgcgata ttttgttcat taccgtttcc
    12361 gtttagcgta tcttctactg ttaaattaac cgcaggcgaa aactcagcca tgacgctgtt
    12421 gaagttgttt ttattgatat caaccttgtc ccgttcggac agcggacgtt gctcttttcc
    12481 gttactgtaa tcgcctacgg caagaagctt gagggggagt tcaactttct ttttagcatt
    12541 gcctgtatgc agatcaagct tgatatttac acgagcggcg ggaacttcat tctggaaact
    12601 gtcggccata tccttttcct catcatccac tttttc
//
LOCUS       DAZFLD010000139        12356 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.140, whole genome shotgun sequence.
ACCESSION   DAZFLD010000139 DAZFLD010000000
VERSION     DAZFLD010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12356
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.140"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(61..834)
                     /gene="uxuR"
                     /locus_tag="ACHKBL_003379"
     CDS             complement(61..834)
                     /gene="uxuR"
                     /locus_tag="ACHKBL_003379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxu operon transcriptional regulator"
                     /protein_id="HFO5820206.1"
                     /translation="MKSATSAQRPYQEVGAMIRDLIIKTPYNPGERLPPEREIAEMLD
                     VTRTVVREALIMLEIKGLVEVRRGAGIYVLDSSGSHNTDSPDANVCNDAGPFELLQAR
                     QLLESNIAEFAALQATREDIVKMRQALQLEERELASSAPGSSESGDMQFHLAIAEATH
                     NSMLVELFRQSWQWRENNPMWIQLHSHLDDSLYRKEWLGDHKQILAALIKKDARAAKL
                     AMWQHLENVKQRLLEFSNVDDIYFDGYLFDSWPLDKVDA"
     gene            complement(1049..2509)
                     /locus_tag="ACHKBL_003380"
     CDS             complement(1049..2509)
                     /locus_tag="ACHKBL_003380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructuronate reductase"
                     /protein_id="HFO5820207.1"
                     /translation="MTTIVDSNLPVARPSWDHSRLESRIVHLGCGAFHRAHQALYTHH
                     LLESTDSDWGICEVNLMPGNDRVLIENLKKQQLLYTVAEKGAESTELKIIGSMKEALH
                     PEIDGCEGILNAMARPQTAIVSLTVTEKGYCADAASGQLDLNNPLIKHDLENPTAPKS
                     AIGYIVEALRLRREKGLKAFTVMSCDNVRENGHVAKVAVLGLAQARDPQLAAWIEENV
                     TFPCTMVDRIVPAATPETLQEIADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDKV
                     GAQFVADVVPFEMMKLRMLNGSHSFLAYLGYLGGYETIADTMTNPAYRKAAFALMMQE
                     QAPTLSMPEGTDLNAYATLLIERFSNPSLRHRTWQIAMDGSQKLPQRLLDPVRLHLQN
                     GGSWRHLALGVAGWMRYTQGVDEQGNAIDVVDPMLAEFQKINAQYQGADRVKALLGLS
                     GIFADDLPQNADFVGAVTAAYQQLCERGARECVAAL"
     gene            complement(2590..3774)
                     /gene="uxuA"
                     /locus_tag="ACHKBL_003381"
     CDS             complement(2590..3774)
                     /gene="uxuA"
                     /locus_tag="ACHKBL_003381"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFO5820208.1"
                     /translation="MEQTWRWYGPNDPVSLADVRQAGATGVVTALHHIPNGEVWSVEE
                     ILKRKAIVEDAGLVWSVVESVPIHEDIKTHTGNYEQWIANYQQTLRNLAQCGIRTVCY
                     NFMPVLDWTRTDLEYVLPDGSKALRFDQIEFAAFEMHILKRPGAEADYTEEEIAQAAV
                     RFATMSDEDKARLTRNIIVGLPGAEEGYTLDQFRKHLELYKDIDKAKLRENFAVFLKA
                     IIPVAEEVGVRMAVHPDDPPRPILGLPRIVSTIEDMQWMVDTVNSMANGFTMCTGSYG
                     VRADNDLVDMIKQFGPRIYFTHLRSTMREDNPKTFHEAAHLNGDVDMYEVVKAIVEEE
                     HRRKAEGKEDLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLKGLAEVRGVELAIQRAFF
                     SR"
     gene            4114..5457
                     /gene="gntP"
                     /locus_tag="ACHKBL_003382"
     CDS             4114..5457
                     /gene="gntP"
                     /locus_tag="ACHKBL_003382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate permease GntP"
                     /protein_id="HFO5820209.1"
                     /translation="MHVLNILWVVFGIGLMLVLNLKFKINSMVALLVAALSVGMLAGM
                     DLMSLLHTMKAGFGNTLGELAIIVVFGAVIGKLMVDSGAAHQIAHTLLARLGLRYVQL
                     SVIIIGLIFGLAMFYEVAFIMLAPLVIVIAAEAKIPFLKLAIPAVAAATTAHSLFPPQ
                     PGPVALVNAYGADMGMVYIYGVLVTIPSVICAGLILPKFLGNLERPTPSFLKADQPVD
                     MNNLPSFGVSILVPLIPAIIMISTTIANIWLVKDTPAWEVVNFIGSSPIAMFIAMVVA
                     FVLFGTARGHDMQWVMNAFESAVKSIAMVILIIGAGGVLKQTIIDTGIGDTIGMLMSH
                     GNISPYIMAWLITVLIRLATGQGVVSAMTAAGIISAAILDPATGQLVGVNPALLVLAT
                     AAGSNTLTHINDASFWLFKGYFDLSVKDTLKTWGLLELVNSVVGLIIVLIISMVA"
     gene            complement(5630..6532)
                     /gene="fimH"
                     /locus_tag="ACHKBL_003383"
     CDS             complement(5630..6532)
                     /gene="fimH"
                     /locus_tag="ACHKBL_003383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HFO5820210.1"
                     /translation="MKRVIYLFAVLLMGWSVNAWSFACKTANGTAIPIGGGSANVYVN
                     LAPAVNVGQNLVVDLSTQIFCHNDYPETITDYVTLQRGSAYGGVLSNFSGTVKYSGSS
                     YPFPTTSETPRVVYNSRTDKPWPVALYLTPVSSAGGVAIKAGSLIAVLILRQTNNYNS
                     DDFQFVWNIYANNDVVVPTGGCDVSARDVTVTLPDYPGSVPIPLTVYCAKSQNLGYYL
                     SGTTADAGNSIFTNTASFSPAQGVGVQLTRNGTIIPANNTVSLGAVGTSAVSLGLTAN
                     YARTGGQVTAGNVQSIIGVTFVYQ"
     gene            complement(6552..7055)
                     /gene="fimG"
                     /locus_tag="ACHKBL_003384"
     CDS             complement(6552..7055)
                     /gene="fimG"
                     /locus_tag="ACHKBL_003384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimG"
                     /protein_id="HFO5820211.1"
                     /translation="MKWRKRGYLLAAMLALASATIQAADVTITVNGKVVAKPCTVSTT
                     NATVDLGDLYSFSLMSAGAASAWHDVALELTNCPVGTSRVTAGFSGAADSTGYYKNQG
                     TAQNIQLELQDDSGNTLNSGATKTVQVDDSSQSAHFPLQVRALTVNGGATQGTIQAVI
                     SITYTYS"
     gene            complement(7068..7598)
                     /gene="fimF"
                     /locus_tag="ACHKBL_003385"
     CDS             complement(7068..7598)
                     /gene="fimF"
                     /locus_tag="ACHKBL_003385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimF"
                     /protein_id="HFO5820212.1"
                     /translation="MRNKPFYLLCAFLWLAVSHALAADSTITIRGYVRDNGCSVAAES
                     TNFTVDLMENAVKQFNNIGATTPVVPFRILLSPCGNAVSAVKVGFTGVADSHNANLLA
                     LENTVSAAAGLGIQLLNEQQNQIPLNAPSSAISWTTLTPGKPNTLNFYARLMATQVPV
                     TAGHINATATFTLEYQ"
     gene            complement(7608..10244)
                     /gene="fimD"
                     /locus_tag="ACHKBL_003386"
     CDS             complement(7608..10244)
                     /gene="fimD"
                     /locus_tag="ACHKBL_003386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher FimD"
                     /protein_id="HFO5820213.1"
                     /translation="MSYLNLRLYQRNTQCLHIRKHRLAGFFVRLFVACAFAAQAPLSS
                     AELYFNPRFLADDPQAVADLSRFENGQELPPGTYRVDIYLNNGYMATRDVTFNTGDSE
                     QGIVPCLTRAQLASMGLNTASVSGMNLLADDACVPLTSMIHDATAHLDVGQQRLNLTI
                     PQAFMSNRARGYIPPELWDPGINAGLLNYNFSGNSVQNRIGGNSHYAYLNLQSGLNIG
                     AWRLRDNTTWSYNSSDSSSGSKNKWQHINTWLERDIIPLRSRLTLGDGYTQGDIFDGI
                     NFRGAQLASDDNMLPDSQRGFAPVIHGIARGTAQVTIKQNGYDIYNSTVPPGPFTIND
                     IYAAGNSGDLQVTIKEADGSTQIFTVPYSSVPLLQREGHTRYSITAGEYRSGNAQQEK
                     PRFFQSTLLHGLPAGWTIYGGTQLADRYRAFNFGIGKNMGALGALSVDMTQANSTLPD
                     DSQHDGQSVRFLYNKSLNESGTNIQLVGYRYSTSGYFNFADTTYSRMNGYNIETQDGV
                     IQVKPKFTDYYNLAYNKRGKLQLTVTQQLGRSSTLYLSGSHQTYWGTSNVDEQFQAGL
                     NTAFEDINWTLSYSLTKNAWQKGRDQMLALNVNIPFSHWLRSDSKSQWRHASASYSMS
                     HDLNGRMTNLAGVYGTLLEDNNLSYSVQTGYAGGGDGNSGSTGYATLNYRGGYGNANI
                     GYSHSDDIKQLYYGVSGGVLAHANGVTLGQPLNDTVVLVKAPGAKDAKVENQTGVRTD
                     WRGYAVLPYATEYRENRVALDTNTLADNVDLDNAVANVVPTRGAIVRAEFKARVGIKL
                     LMTLTHNNKPLPFGAMVTSESSQSSGIVADNGQVYLSGMPLAGKVQVKWGEEENAHCV
                     ANYQLPPESQQQLLTQLSAECR"
     gene            complement(10311..11036)
                     /gene="fimC"
                     /locus_tag="ACHKBL_003387"
     CDS             complement(10311..11036)
                     /gene="fimC"
                     /locus_tag="ACHKBL_003387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418736.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HFO5820214.1"
                     /translation="MSNKNVNVRKSQEITFCLLAGILMFMAMMVAGRAEAGVALGATR
                     VIYPAGQKQVQLAVTNNDENSTYLIQSWVENADGVKDGRFIVTPPLFAMKGKRENTLR
                     ILDATNNQLPQDRESLFWMNVKAIPSMDKSKLTENTLQLAIISRIKLYYRPAKLALPP
                     DQAAEKLRFRRSANSLTLINPTPYYLTVTELNAGTRVLENALVPPMGESTVKLPSDAG
                     SNITYRTINDYGALTPKMTGVME"
     gene            complement(11073..11612)
                     /locus_tag="ACHKBL_003388"
     CDS             complement(11073..11612)
                     /locus_tag="ACHKBL_003388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO5820215.1"
                     /translation="MKRKRLFLLASLLPMFALAGNKWNTTLPGGNMQFQGVIIAETCR
                     VEAGDKQMTVNMGKISSNRFHAVGEDSAPVPFVIHLRECSTVVSERVGVAFHGVADGK
                     NPDVLSVGEGPGIATNIGVALFDDEGNLVPINRPPANWKRLYSGSTSLHFIAKYRATG
                     RRVTGGIANAQAWFSLTYQ"
     gene            complement(11677..12231)
                     /gene="fimA"
                     /locus_tag="ACHKBL_003389"
     CDS             complement(11677..12231)
                     /gene="fimA"
                     /locus_tag="ACHKBL_003389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HFO5820216.1"
                     /translation="MKIKTLAIVVLSALSLSSTAALAETTPTTVNGGTVHFKGEVVNA
                     ACAVDAGSVDQTVQLGQVRTASLAQEGATSSAVGFNIQLNDCDTTVADKAAIAFLGTA
                     IDGTHPNVLALQSSAAGSATKVGVQILDRTGAALALDGATFSAQTTLNNGTNTIPFQA
                     RYYAIGEATPGAANADATFKVQYQ"
BASE COUNT         2922 a         3262 c         3023 g         3149 t
ORIGIN      
        1 taactattca gggaacatac atagtgcaaa gcggttcaac tttgcaccag aaatgaggga
       61 tcaggcgtcg actttatcca gcggccatga atcaaacaga tagccgtcaa aataaatatc
      121 gtcaacgttc gagaattcca gcagacgttg tttaacgttt tccagatgct gccacattgc
      181 cagcttcgcc gcacgggcat cttttttgat taacgcggcg aggatctgtt tgtgatcgcc
      241 caaccactct ttgcgataca ggctgtcatc gagatggctg tgcaattgga tccacattgg
      301 attgttttcc cgccactgcc aggactgacg gaataactcc accagcatgc tgttatgcgt
      361 tgcttcggca atggcgagat ggaactgcat atcaccgctt tcgctgctac ccggcgcgct
      421 ggaagccagt tcacgctctt ccagttgcaa tgcctgacgc attttgacga tatcttcgcg
      481 ggtagcctgc aaagcggcaa attccgcgat gttactttcc agtaactgac gcgcctgtaa
      541 cagctcaaaa ggaccggcat cattacagac gttggcatcc ggactgtctg tgttgtggct
      601 gcctgagcta tcaagaacat aaataccggc accccggcgc acttccacca gccctttgat
      661 ctccagcatg atcagcgctt cacgtaccac cgtccgcgtg acatcaagca tttctgcaat
      721 ttcacgctcc ggtggcagcc gttcgccagg attgtacggc gtctttatga tcagatcgcg
      781 gatcatcgcc ccgacttcct ggtaaggtct ttgcgcagag gtggcagatt tcatggtgtt
      841 gtgtccgtta ggtgcggtta atcaaatttt ttccggatgc ggcgtgaacg ccttatccga
      901 cctacaacgg gcaattttac ggcacccgtt aagctcaaac tataccatta gcactggatg
      961 ttggcacgta caggtgggca aaaaaacgaa aactggtcaa ccaaatatta gtttgattga
     1021 ccagtaagtc tgtagggtaa tcagttagtt acagcgcagc cacacactcg cgcgcaccgc
     1081 gctcacacag ctgctgatat gccgccgtca ccgcgccaac aaagtcggcg ttctgcggca
     1141 gatcatcggc aaagataccg ctcaggccca gcagtgcttt cacgcggtct gcaccctgat
     1201 actgcgcgtt gatcttctgg aactccgcca gcatcgggtc aaccacgtca atggcattac
     1261 cctgctcatc cacgccctgg gtgtaacgca tccagcccgc cacgcccagc gccaggtgac
     1321 gccagctacc gccgttttgc aggtgcagac gcaccgggtc cagcagacgc tgcggtaact
     1381 tctggctgcc gtccattgca atctgccagg tacggtgacg cagagacggg ttgctgaaac
     1441 gctcgatcag cagcgtcgca taagcgttca ggtctgtacc ttccggcatc gacagcgttg
     1501 gcgcttgttc ctgcatcatc agggcaaagg ccgctttgcg ataagccggg ttagtcatgg
     1561 tgtcggcaat agtttcatag ccgccgaggt aaccgaggta cgccaggaaa gagtggctac
     1621 cgttcagcat acgcagcttc atcatttcga acggtacaac atccgcaaca aactgcgcgc
     1681 cgactttatc ccagtccgga cgaccattaa cgaagttatc ttcaattacc cactgacgga
     1741 acggttcgca ggcaatggcg cacgggtcgt aaacacccag ctggtcagca atttcctgta
     1801 aggtttctgg cgtcgccgcc ggaacgatgc ggtcaaccat ggtgcacggg aaggtgacgt
     1861 tctcttcaat ccatgccgcc agttgcgggt cacgcgcctg agccagccca agaacggcaa
     1921 ccttcgccac atgaccgttt tcacgcacgt tatcgcagga catcaccgta aatgctttca
     1981 gccctttttc acgacgcaga cgcagggctt ctacgatgta accaatcgca gacttcggcg
     2041 cagtcgggtt ttccagatcg tgcttgatca gcgggttatt gagatccagt tgaccgcttg
     2101 ccgcatctgc gcagtagcct ttttccgtga ccgtcagaga gacaatcgcc gtctgcggac
     2161 gcgccatcgc gttgagaata ccttcgcaac cgtcgatttc cgggtgcagg gcttctttca
     2221 tcgaaccgat aattttcagc tcggtgcttt ctgcgccttt ttccgccacg gtgtacagca
     2281 gttgctgttt tttcaggttt tcgatcagca cgcggtcgtt gcctggcatc aggttaactt
     2341 cgcagatccc ccagtcgcta tccgtgcttt ccagcaggtg atgagtgtac agcgcctggt
     2401 gcgcgcggtg aaacgccccg caaccgagat gcacaatgcg tgattccaga cgagaatgat
     2461 cccatgacgg gcgggcaacc ggcagattgc tgtcaacaat agtagtcatc gtaaactcca
     2521 gaatccgtct tcagagagtg acggcaagga agaaggaacc ggcacggcgc gcagccatgc
     2581 cggtggatat taacggctaa agaaagcgcg ctggatcgcc agttcgacac cgcgaacttc
     2641 ggccaggcct ttcagacgac caattgcgga gtaaccagga ttggttttct tcttcaggtc
     2701 gtccagcatc tgatgaccgt ggtccggacg catcgggatc aggtcttctt tgccttccgc
     2761 tttacgacgg tgttcttctt caacaatcgc tttcaccact tcgtacatat caacgtcacc
     2821 gttcaggtgc gccgcttcgt ggaaggtttt cgggttatct tcacgcatgg tggagcgcag
     2881 atgggtgaag taaatacgcg gaccgaactg cttgatcata tcaaccagat cgttgtcagc
     2941 acgcacgccg taggaaccgg tgcacatggt gaaaccgttc gccatgctgt ttacggtatc
     3001 aaccatccac tgcatatctt caatggtgga aacaatgcgc ggcaggccga ggatcgggcg
     3061 cggcggatcg tccgggtgaa ccgccatacg cacgccaact tcttcagcaa ctggaataat
     3121 cgctttcagg aagacagcga agttttcgcg cagcttcgct ttgtcgatat ctttgtacag
     3181 ctccaggtgc ttacggaact ggtcgagggt atacccttct tccgcacctg gcagaccgac
     3241 aatgatgtta cgggtcagac gcgctttgtc ttcgtcgctc atagtggcga agcgaacagc
     3301 tgcctgagca atttcttctt cggtgtaatc cgcttccgcg cctggacgct tcaggatatg
     3361 catttcgaat gcagcgaatt cgatctggtc gaagcgcaga gctttggagc cgtctggcag
     3421 cacgtattcg aggtcagtac gggtccagtc gagcaccggc atgaagttgt agcacacggt
     3481 gcgaatgccg cactgcgcca ggttgcgcag ggtctgctga tagttagcaa tccactgctc
     3541 atagttgcca gtgtgggttt tgatatcttc gtgaattggt acgctttcaa cgacagacca
     3601 caccaggcct gcgtcttcaa cgatcgcctt gcgtttgagg atctcttcta cggaccatac
     3661 ttcgccgttc gggatatggt gcagcgcggt aaccacgcca gttgcgcccg cctgacggac
     3721 atcagctaaa gaaaccggat cgtttgggcc gtaccagcgc caggtctgtt ccatgatttt
     3781 tcctctttca catagtgggt tccacagcaa agagacgtgg agccccggag ttgtttaggt
     3841 atccggatga acaaccagac cgcagccgtg gcgttgcatc ggatgagatg gcgtataagt
     3901 tctacctttt ggtaagacat gtcaacccga aatctgaaat tggttaacca catcacaaga
     3961 atttcactct cctggttgtt gctgcaaatt gcagcaaagg gtaatcgatg gttattgatc
     4021 agaggatgtg acattcatcg caacaatggt tgaccaattt acataacatg tcgcccaaaa
     4081 taacaacggt tcaaccaggt aaggtaaaac gttatgcatg tgcttaacat tctctgggtg
     4141 gtattcggca ttggtctgat gctggtactg aatttgaagt tcaaaatcaa ttcaatggtg
     4201 gctttgttgg tggcggcgct gtccgtcggg atgctggcgg gcatggattt gatgtcgctg
     4261 ctgcacacca tgaaagcggg cttcggcaac acgctggggg aactggctat catcgtggtg
     4321 ttcggtgcgg tcatcggtaa attgatggtc gactccggcg cggctcacca gatagcacat
     4381 acgctgctgg cgcgtctcgg tctgcgctat gtacagctgt cggtgattat catcggcctg
     4441 attttcggtc tggcgatgtt ttatgaagtg gcctttatca tgttagcgcc gctggttatt
     4501 gttattgccg ccgaagctaa aattccgttc ctgaaactgg cgatcccggc agtagcagct
     4561 gccactaccg cacattcact gttcccaccg cagccgggtc cggtggcact ggtgaatgct
     4621 tatggcgcgg atatggggat ggtttatatc tatggcgtac tggtgacgat cccaagtgtc
     4681 atctgcgcag gtctgatcct gccgaagttc ctcggcaatc ttgagcgccc aacgccatca
     4741 ttcctgaaag cagatcaacc ggtagatatg aataatctgc cctctttcgg cgtttcgatt
     4801 ctggtgccgc tgatcccggc gatcattatg atctctacca ccatcgccaa tatctggctg
     4861 gtaaaagata cccctgcctg ggaagtggtt aactttatcg gttcctcgcc gattgcaatg
     4921 tttattgcga tggtggttgc attcgtactc tttggcaccg cgcgtggtca tgacatgcag
     4981 tgggtgatga acgcttttga aagcgcggtg aaaagcattg caatggtgat tctgatcatc
     5041 ggtgcgggtg gcgtgctgaa gcagaccatc atcgacaccg gcattggcga caccatcggc
     5101 atgttgatgt cccacggcaa tatctcgccc tacatcatgg catggctgat caccgtactg
     5161 attcgtctgg cgacgggtca gggtgtcgtt tcggcgatga ccgctgccgg gattatcagt
     5221 gctgcaatcc ttgatccagc aaccggtcag ctggttggcg tgaatccggc gctgctggtg
     5281 ctggcgacgg ctgcgggttc caacaccctc acgcacatta acgatgcatc tttctggttg
     5341 ttcaaaggtt actttgacct gtcagtaaaa gacacgttga aaacctgggg tctgctggag
     5401 ctggtcaact ccgtggttgg gctgattatt gtgttgatta ttagcatggt agcgtaaaag
     5461 aacaccggag cctgccaatg gcaggctctg ttcggattgt cggtaaagtg cgctttgttt
     5521 atgccagatg cgacgctgac gcgtcttatc tggcatacaa agggctaacg tgcaggtttt
     5581 agcttcaggt aatattgcgt acctgcatta gcaatgccct gtgatttctt tattgataaa
     5641 caaaagtaac gccaataatc gattgcacat tccctgcagt cacctgccct ccggtacgtg
     5701 cgtaatttgc cgttaatccc agacttaccg ccgaagtccc cactgctcct aacgataccg
     5761 tgttattcgc tggaataatc gtaccgttgc gcgtcaactg tacgccgacg ccctgtgctg
     5821 gtgaaaacga ggcggtattg gtgaaaatcg agttgcccgc atctgcggtt gtgccggaga
     5881 ggtaataccc caggttttgg cttttcgcac aataaacggt aagaggaatc ggcaccgaac
     5941 cgggatagtc cggcagagta acggtgacat cacgagcaga aacatcacag ccgccagtag
     6001 gcaccaccac atcattattg gcgtaaatat tccacacaaa ctggaaatca tcgctgttat
     6061 agttgttggt ctgtcgcaaa attagcacgg caattaatga gccagcttta atcgccaccc
     6121 cgcccgcact gctcacaggc gtcaaataaa gcgccaccgg ccacggctta tccgttctcg
     6181 aattataaac aacgcgcggc gtttcgctag tggtcggaaa tggatagcta ctgccactat
     6241 attttacggt cccggaaaaa ttagataaca cgccgccata agccgagcct cgttgcagtg
     6301 tgacatagtc tgtaatggtt tccggataat cgttatggca aaagatttgc gtcgaaagat
     6361 ccacgaccag gttttgcccc acattcacgg caggcgcaag gtttacataa acattagcgc
     6421 tgccaccgcc aatagggata gcggtgccat tggcggtttt acaggcgaat gaccaggcat
     6481 ttaccgacca gcccatcagc agtacagcaa acaggtaaat aactcgtttc attacaatca
     6541 tctcttcggc ttcagctgta ggtataggtg atgctaatta ctgcctgaat ggttccctga
     6601 gtggctccgc catttaccgt caatgctctg acctgtaacg ggaagtgcgc tgattgtgag
     6661 gaatcatcca cctgaactgt tttggttgcg ccactattca atgtgttgcc actgtcatcc
     6721 tgtagctcta actggatgtt ttgcgcggtc ccctggtttt tataatatcc ggtactgtcg
     6781 gctgccccgc tgaagccggc ggtgaccctc gacgttccca ccggacaatt agtcaactca
     6841 agcgcaacat catgccaggc cgatgccgcc ccggcagaca tcagactgaa agaataaaga
     6901 tcgccgagat ctaccgtggc attggtggtg gaaaccgtgc acggtttggc gacgacctta
     6961 ccgttcaccg tgatggtgac atcggctgcc tgtatcgtcg cacttgcgag cgccaacatt
     7021 gccgccaata aatacccacg tttgcgccat ttcatgagca tctccagtta ctgatattca
     7081 agagtgaagg tagccgtggc attgatatgc cccgcagtga caggcacctg tgtcgccatt
     7141 agccgggcgt aaaaattcag cgtatttggt ttacccggcg tcagggtcgt ccacgaaatc
     7201 gcggacgatg gggcattaag gggtatctga ttttgttgct cattcagaag ctgtattccc
     7261 agtcccgcag ccgctgacac cgtattttca agtgcaagca ggttggcatt gtggctatct
     7321 gcaacaccgg taaacccaac ttttacggca gaaacggcat taccacaggg tgacagcaaa
     7381 atacgaaatg gaacgacagg agtcgtcgcg ccaatgttgt taaattgctt caccgcgttt
     7441 tccatcagat caacagtaaa attggttgat tcagcggcca cactacagcc attatctctg
     7501 acatagccgc ggatagtaat cgtgctatcc gcagccaaag cgtgacttac cgccagccac
     7561 aaaaaagcgc aaagaagata aaaaggtttg tttctcatca cgcctcctta acgacattca
     7621 gctgatagct gggttaataa ctgctgctga ctctctggtg gcagttgata attggcgaca
     7681 cagtgagcat tttcctcttc tccccatttc acctgaactt ttcccgctaa aggcattccg
     7741 ctgaggtaaa cctgaccatt atccgcaaca atgccgctac tctggctact ctctgatgtc
     7801 accatcgccc caaacggcag cggcttatta ttgtgggtta gcgtcatgag cagttttatc
     7861 ccaacgcgcg ctttaaactc tgctcgcacg atcgccccac gggtgggaac aacgttagcg
     7921 accgcgttat ctaaatcgac gttatcagcc agggtattgg tatccagcgc tactctgttt
     7981 tcccgatatt cagtggcata aggcagcacg gcataaccgc gccagtcggt acgcaccccc
     8041 gtctggtttt cgacttttgc atcttttgcg ccaggcgctt taacaagcac caccgtatcg
     8101 tttaacggct gccccagcgt tacgccattg gcatgagcca gtaccccacc gctgactccg
     8161 taatagagct gcttaatatc atcgctatgg ctgtaaccga tgttggcatt gccgtaacca
     8221 ccgcgataat tcagcgtggc gtagcctgta cttccgctat taccatcacc tcccccggcg
     8281 taaccggttt gcacgctata gctgaggttg ttgtcttcca gcaacgtacc gtatacaccg
     8341 gccagattgg tcatccgacc gttgagatcg tgtgacatgc tgtaactggc actggcatgt
     8401 cgccactgag atttactgtc agaacgcagc cagtggctga aaggaatatt gacgttaagc
     8461 gctaacatct gatcccgtcc tttttgccag gcgtttttcg tcaggctata gctgagcgtc
     8521 cagttgatat cttcgaatgc agtatttaaa ccagcctgga attgctcatc gacattactc
     8581 gttccccaat aagtttgatg gctaccactc aaatacagtg ttgatgagcg cccgagttgc
     8641 tgagtaacgg tgagctgtaa tttcccgcgt ttgttataag cgagattgta atagtcggtg
     8701 aatttcggct taacctgaat aactccatcc tgtgtttcga tgttgtagcc attcattcga
     8761 ctgtatgttg tatcagcgaa attaaaatac ccactggtcg aataacggta acccactaac
     8821 tgaatattcg tgcctgactc attgagcgat ttgttataaa gaaaacgcac cgattgtccg
     8881 tcatgctgac tgtcatcggg aagtgtggaa ttagcctgcg tcatatccac agacagagcg
     8941 cccagtgccc ccatattttt cccgataccg aaattaaacg cacgataacg atccgccagt
     9001 tgcgttccac catatattgt ccagccagcc ggaaggccgt ggagtaatgt actctggaaa
     9061 aagcggggtt tttcctgttg cgcatttcca ctacggtatt ctcctgccgt aatggaataa
     9121 cgtgtatgcc cttcacgctg caaaagcggg actgacgaat agggtacggt aaaaatctgc
     9181 gtactgccgt cagcctcttt aatcgttacc tgcaagtcac cactattacc tgcggcatag
     9241 atatcgttga tggtaaaagg ccccggtggc accgtactat tataaatgtc atacccattt
     9301 tgtttaatag tgacctgtgc agtaccacga gcaataccgt ggatcaccgg ggcaaatcct
     9361 ctttggctat cgggtaacat attgtcatct gaggccaatt gtgcgccgcg aaagttaata
     9421 ccatcgaaaa tatcgccctg agtataacca tcacccagcg tcagccggga acgtaacgga
     9481 attatgtctc gctcaagcca ggtattgata tgctgccatt tatttttgct acctgatgaa
     9541 ctgtcgctac tgttataact ccaggtggta ttgtcgcgta aacgccacgc accaatattt
     9601 aacccactct gtaggtttaa atatgcataa tggctgttac ccccaattcg attctgtaca
     9661 ctatttccgc tgaaattata attgagcaat ccggcattaa taccgggatc ccataactca
     9721 ggaggaatat aaccacgcgc gcgattactc ataaatgcct gagggatcgt caggttgagt
     9781 cgctgctgac caacatctaa atgcgcagta gcgtcatgga tcattgaggt taatggcacg
     9841 caggcatcat ccgccagcag attcataccg gagacagaag ccgtattcag ccccatactg
     9901 gcaagttgcg cgcgtgtcag gcagggaaca atcccttgtt cactgtcgcc cgtgttaaat
     9961 gtgacatcac gcgttgccat ataaccatta ttcaaataga tatcgacgcg atacgtccct
    10021 ggcggtaatt cttgcccatt ttcaaaacgc gataaatcgg ccacagcctg gggatcatcc
    10081 gctaaaaagc gcggattaaa atagagttcg gcagatgaca aaggtgcctg tgcggcaaaa
    10141 gcacaggcga caaagagccg gacaaaaaaa ccagccaaac gatgcttacg aatatgcaag
    10201 cattgtgtgt ttcgctggta aagtcttaaa ttcagatatg acattattcc tccggtttaa
    10261 aatcacccgg cagtcaattc tttttattca ggcgaaaaat tccccctgcg ttattccatt
    10321 acgcccgtca ttttgggggt aagtgcgcca taatcattta ttgttcggta agtaatattg
    10381 cttcctgcat cagaaggcaa tttaaccgtg ctttcgccca ttggaggcac caatgcattt
    10441 tcaagaaccc gggttccggc attcaactct gttaccgtca ggtaataggg tgtcgggtta
    10501 atcagcgtca gagaattcgc gctacgacga aatcttaatt tttctgcggc ctgatcgggt
    10561 ggcaacgcta atttagccgg gcgatagtac agcttaatgc ggctgataat tgcgagctgt
    10621 agcgtattct cagtcaattt tgatttatcc attgacggaa tcgctttaac gttcatccag
    10681 aataaacttt cccgatcctg tggcaattgg ttatttgttg catcaagaat acgtaaggta
    10741 ttctctcttt ttcccttcat cgcaaacaga ggtggcgtca cgataaaacg accatccttt
    10801 acaccatcgg cattttccac ccatgattga attaaatagg tgctattttc atcattattt
    10861 gtcacggcaa gttgcacttg tttttgccct gccggataaa ttacgcgggt cgcacctaag
    10921 gccactcccg cttcagcgcg tccggcaacc atcattgcca tgaacatcag gatacctgcc
    10981 agcaagcaga atgttatttc ctgcgatttc cttacattga cgtttttatt actcactgtc
    11041 ctgttcctgt tatcacatta tctgctgaac aattactgat aggttaaaga gaaccaggcc
    11101 tgggcattgg cgatgccgcc agtaacccga cgcccggtag cacgatattt ggcgatgaaa
    11161 tgtagcgaag tagagcctga gtaaagccgt ttccagtttg ctggaggacg attaatcggt
    11221 acgaggtttc cttcatcatc aaataacgct acgccaatat tggtggctat ccctggcccc
    11281 tctcccacgg aaagcacatc cggattttta ccatccgcga caccgtgaaa cgccacaccc
    11341 acacgttcac tcaccaccgt gctacattcc cgtaaatgaa taacaaaagg caccggtgcg
    11401 ctatcttccc ctaccgcatg aaaccggtta ctgctgattt tccccatatt gaccgtcatt
    11461 tgtttatcac cagcttcaac tcggcaagtt tccgcaataa tgacgccctg aaattgcata
    11521 tttccgccgg gcagcgtggt attccattta tttccggcca gagcaaacat tggcagcaac
    11581 gacgctaata gaaataatct cttccttttc atcgttattt ttatcgcaca agggtgggca
    11641 tccctgcccg taatgacgtc cctgaacctg ggtaggttat tgatactgaa ccttgaaggt
    11701 cgcatccgca ttagcagcac ccggggttgc ctcgccgatt gcataataac gcgcctggaa
    11761 cggaatggtg ttggtaccgt tattcaaggt tgtttgtgca ctaaatgtcg caccgtccag
    11821 cgccagcgca gcacccgttc tgtccaggat ctgcacacca acttttgttg cgctacccgc
    11881 agctgaactc tgcaaagcta atacgttagg atgagttcca tcaattgcgg taccaaggaa
    11941 ggcaatagca gctttgtctg caacagtagt gtcacaatcg ttcagttgaa tgttaaaacc
    12001 gacagcagaa ctggttgctc cttcttgtgc cagcgatgct gtacgaacct gccctaactg
    12061 aacagtttga tcaacagagc ctgcatcaac tgcgcaagcg gcgttaacaa cctccccttt
    12121 aaagtgaacg gtcccaccat ttaccgtcgt gggtgtagtt tcagccagag ccgctgtaga
    12181 actcagggac agagccgaca gaacaacaat tgccagagtt ttaattttca tactgctttc
    12241 ctttcaaaaa actatttcta aatcgacatg ggcagtcgtt ctgtacactt tgttttgtca
    12301 acgagtttga ctgccaacac tgcacagttt tcccccaaaa gatgaaacat ttgggg
//
LOCUS       DAZFLD010000140        12318 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.141, whole genome shotgun sequence.
ACCESSION   DAZFLD010000140 DAZFLD010000000
VERSION     DAZFLD010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12318
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.141"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..387)
                     /locus_tag="ACHKBL_003390"
     CDS             complement(<1..387)
                     /locus_tag="ACHKBL_003390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Arm DNA-binding domain-containing protein"
                     /protein_id="HFO5820217.1"
                     /translation="MALTDRAIVHAKPCGKPYKLSDSHGLYLLVNPNGSKRWYIKYRF
                     VNKEKKLALGPYPLLTLAQARRMREEAQLLLISGIDPSARRKAERLAITPEHTFESVA
                     REWVTSNVNWSAEHKKRVLRYFELYVF"
     gene            complement(610..694)
                     /locus_tag="ACHKBL_003391"
     tRNA            complement(610..694)
                     /locus_tag="ACHKBL_003391"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(658..660),aa:Leu,seq:caa)
     gene            890..1909
                     /gene="ahr"
                     /locus_tag="ACHKBL_003392"
     CDS             890..1909
                     /gene="ahr"
                     /locus_tag="ACHKBL_003392"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase Ahr"
                     /protein_id="HFO5820218.1"
                     /translation="MSMIKSYAAKEAGGELEVYEYDPGELKPQDVEVQVDYCGICHSD
                     LSMIDNEWGFSQYPLVAGHEVIGRVVALGSAAQDKGLQVGQRVGIGWTARSCGHCDAC
                     ISGNQINCEQGAVPTILNRGGFAEKLRADWQWVIPLPENIDIESAGPLLCGGITVFKP
                     LLMHHITATSRVGVIGIGGLGHIAIKLLHAMGCEVTAFSSNPAKEQEVLAMGADKVVN
                     SRDPQALKALAGQFDLIINTVNVSLDWQPYFEALTYGGNFHTVGAVLTPLSVPAFTLI
                     AGDRSVSGSATGTPYELRKLMRFAARSKVAPTTELFPMSKINDAIQHVRDGKARYRVV
                     LKADF"
     gene            2039..3541
                     /gene="yjgR"
                     /locus_tag="ACHKBL_003393"
     CDS             2039..3541
                     /gene="yjgR"
                     /locus_tag="ACHKBL_003393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase HerA-like C-terminal domain-containing
                     protein"
                     /protein_id="HFO5820219.1"
                     /translation="MSEPLLIARTSDTELFLLPGMANRHGLITGATGTGKTVTLQKLA
                     ESLSEIGVPVFMADVKGDLTGVAEEGTSSEKLLARLKNIGVNDWQPHANPVVVWDIFG
                     EKGHPVRATVSDLGPLLLARLLNLNDVQSGVLNIIFRIADDQGLLLLDFKDLRAITQY
                     IGDNAKSFQNQYGNISSASVGAIQRGLLSLEQQGAAHFFGEPMLDIKDWMRTDANGKG
                     VINILSAEKLYQMPKLYAASLPWMLSELYEQLPEAGDLEKPKLVFFFDEAHLLFNDAP
                     QVLLDKIEQVIRLIRSKGVGVWFVSQNPSDIPDNVLGQLGNRVQHALRAFTPKDQKAV
                     KAAAQTMRANPAFDTEKAIQELGTGEALISFLDAKGSPSVVERAMVIAPCSRMGPVTE
                     DERNGLINHSPVYGKYEDEVDRESAYEMLQKGFQASTEQQNNPPVKGKEVAVDDGILG
                     GLKDILFGTTGPRGGKKDGVVQTMAKSAARQVTNQIVRGMLGSLLGGRKR"
     gene            complement(3660..4742)
                     /gene="lptG"
                     /locus_tag="ACHKBL_003394"
     CDS             complement(3660..4742)
                     /gene="lptG"
                     /locus_tag="ACHKBL_003394"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313266.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HFO5820220.1"
                     /translation="MQPFGVLDRYIGKTIFTTIMMTLFMLVSLSGIIKFVDQLKKAGQ
                     GSYDALGAGMYTLLSVPKDVQIFFPMAALLGALLGLGMLAQRSELVVMQASGFTRMQV
                     ALSVMKTAIPLVLLTMAIGEWVAPQGEQMARNYRAQAMYGGSLLSTQQGLWAKDGNNF
                     VYIERVKGDEELGGISIYAFNENRRLQSVRYAATAKFDPEHKVWRLSQVDESDLTNPK
                     QITGSQTVSGTWKTNLTPDKLGVVALDPDALSISGLHNYVKYLKSSGQDAGRYQLNMW
                     SKIFQPLSVAVMMLMALSFIFGPLRSVPMGVRVVTGISFGFVFYVLDQIFGPLTLVYG
                     IPPIIGALLPSASFFLISLWLLMRKS"
     gene            complement(4742..5842)
                     /gene="lptF"
                     /locus_tag="ACHKBL_003395"
     CDS             complement(4742..5842)
                     /gene="lptF"
                     /locus_tag="ACHKBL_003395"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HFO5820221.1"
                     /translation="MIIIRYLVRETLKSQLAILFILLLIFFCQKLVRILGAAVDGDIP
                     ANLVLSLLGLGVPEMAQLILPLSLFLGLLMTLGKLYTESEITVMHACGLSKAVLVKAA
                     MILAVFTAIVAAVNVMWAGPWSSRHQDEVLAEAKANPGMAALAQGQFQQATNGSSVLF
                     IESVDGSDFKDVFLAQIRPKGNARPSVVVADSGHLTQLRDGSQVVTLNQGTRFEGTAL
                     LRDFRITDFQDYQAIIGHQAVALDPNDTDQMDMRTLWNTDTDRARAELNWRITLVFTV
                     FMMALMVVPLSVVNPRQGRVLSMLPAMLLYLLFFLIQTSLKSNGGKGKLDPTLWMWTV
                     NLIYLALAIVLNLWDTVPVRRLRASFSRKGAV"
     gene            complement(5905..5955)
                     /gene="ytgA"
                     /locus_tag="ACHKBL_003396"
     CDS             complement(5905..5955)
                     /gene="ytgA"
                     /locus_tag="ACHKBL_003396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YtgA"
                     /protein_id="HFO5820222.1"
                     /translation="MSIIILATISTTQENK"
     gene            6109..7620
                     /gene="pepA"
                     /locus_tag="ACHKBL_003397"
     CDS             6109..7620
                     /gene="pepA"
                     /locus_tag="ACHKBL_003397"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HFO5820223.1"
                     /translation="MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYIS
                     ALLRRGELEGKPGQTLLLHHVPNVLSERILLIGCGKERELDERQYKQVIQKTINTLND
                     TGSMEAVCFLTELHVKGRNNYWKVRQAVETAKETLYSFDQLKTNKSEPRRPLRKMVFN
                     VPTRRELTSGERAIQHGLAIAAGIKAAKDLGNMPPNICNAAYLASQARQLADSYSKNV
                     ITRVIGEQQMKELGMHSYLAVGQGSQNESLMSVIEYRGNASEDARPIVLVGKGLTFDS
                     GGISIKPSEGMDEMKYDMCGAAAVYGVMRMVAELQLPINVIGVLAGCENMPGGRAYRP
                     GDVLTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPEAVIDVATLTGACVIALGHHI
                     TGLMANHNPLAHELIAASEQSGDRAWRLPLGDEYQEQLESNFADMANIGGRPGGAITA
                     GCFLSRFTRKYNWAHLDIAGTAWRSGKAKGATGRPVALLAQFLLNRAGFNGEE"
     gene            7754..8197
                     /gene="holC"
                     /locus_tag="ACHKBL_003398"
     CDS             7754..8197
                     /gene="holC"
                     /locus_tag="ACHKBL_003398"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HFO5820224.1"
                     /translation="MKNATFYLLDNDTTVDGLSAVEQLVCEIAAERWRSGKRVLIACE
                     DEKQAYRLDEALWARPAESFVPHNLAGEGPRGGAPVEIAWPQKRSSSPRDILISLRTS
                     FADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK"
     gene            8197..11052
                     /gene="valS"
                     /locus_tag="ACHKBL_003399"
     CDS             8197..11052
                     /gene="valS"
                     /locus_tag="ACHKBL_003399"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HFO5820225.1"
                     /translation="MEKTYNPQDIEQPLYEHWEKQGYFKPNGDESQESFCIMIPPPNV
                     TGSLHMGHAFQQTIMDTMIRYQRMQGKNTLWQVGTDHAGIATQMVVERKIAAEEGKTR
                     HDYGREAFIDKIWEWKAESGGTITRQMRRLGNSVDWERERFTMDEGLSNAVKEVFVRL
                     YKEDLIYRGKRLVNWDPKLRTAISDLEVENRESKGSMWHIRYPLADGAKTADGKDYLV
                     VATTRPETLLGDTGVAVNPEDPRYKDLIGKYVILPLVNRRIPIVGDEHADMEKGTGCV
                     KITPAHDFNDYEVGKRHALPMINILTFDGDIRESAQVFDTKGNESDVYSSEIPAEFQK
                     LERFAARKAVVAAVDALGLLEEIKPHDLTVPYGDRGGVVIEPMLTDQWYVRADVLAKP
                     AVEAVENGDIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAWYDEAGNVYVGRN
                     EDEVRKENNLSADVALRQDEDVLDTWFSSALWTFSTLGWPENTDALRQFHPTSVMVSG
                     FDIIFFWIARMIMMTMHFIKDENGKPQVPFHTVYMTGLIRDDEGQKMSKSKGNVIDPL
                     DMVDGISLPELLEKRTGNMMQPQLADKIRKRTEKQFPNGIEPHGTDALRFTLAALAST
                     GRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEGQDCGFNGGEMTLSLADRWILAEFN
                     QTIKAYREALDSFRFDIAAGILYEFTWNQFCDWYLELTKPVMNGGTEAELRGTRHTLV
                     TVLEGLLRLAHPIIPFITETIWQRVKVLCGITADTIMLQPFPQYDASQVDEAALADTE
                     WLKQAIVAVRNIRAEMNIAPGKPLELLLRGCSADAERRVNENRGFLQTLARLESITVL
                     PADDKGPVSVTKIIDGAELLIPMAGLINKEDELARLAKEVAKIEGEISRIENKLANEG
                     FVARAPEAVIAKEREKLEGYAEAKAKLIEQQAVIAAL"
     gene            complement(11106..12302)
                     /gene="yjgN"
                     /locus_tag="ACHKBL_003400"
     CDS             complement(11106..12302)
                     /gene="yjgN"
                     /locus_tag="ACHKBL_003400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjgN family protein"
                     /protein_id="HFO5820226.1"
                     /translation="MAQVINEMDVPSHSFVFHGTGERYFLICVVNVLLTIITLGIYLP
                     WALMKCKRYLYANMEVNGQRFSYGITGGNVFVSCLVFVFFYFAILMTVSADMPLVGCV
                     LTLLLLVFLIFMAAKGLRYQALMTSLNGVRFSFNCSLKGFWWVTFFLPILMAIGMGTV
                     FFISTKMLPANSSSSVIISVVLMAIVGIVSIGIFNGTLYSLVMSFLWSNTSFGIHRFK
                     VKLDTTYCIKYAILAFLALLPFLAVAGYIIFDQILNAYDSSVYANDDIENLQQFMEMQ
                     RKMIIAQLIYYFGIAVSTSYLTVSLRNHFMSNLSLNDGRIRFRSTLTYHGMLYRMCAL
                     VVISGITGGLAYPLLKIWMIDWQAKNTYLLGDLDDLPLINKEEQPDKGFLASISRGVM
                     PSLPFL"
BASE COUNT         3171 a         3145 c         3257 g         2745 t
ORIGIN      
        1 gaatacgtaa agctcgaaat aacgcagcac ccgcttctta tgttccgccg accagttcac
       61 gttactggtg acccattcac gtgccacaga ctcaaacgta tgttccggcg ttatcgcaag
      121 gcgttctgct tttcggcgtg cactcggatc gatgccggaa atcagcaata attgtgcctc
      181 ttcacgcatg cgtcttgcct gtgcaagcgt caaaagcgga taagggccta aagcgagctt
      241 tttctctttg tttacaaagc ggtacttgat gtaccagcgt tttgaaccgt tagggttaac
      301 cagcaggtaa agtccatgcg agtcactgag cttatacggc ttgccacaag gcttggcgtg
      361 gactatagcg cgatctgtta gtgccatttg ggggcctcac tattatcgaa ctacgtctaa
      421 gcccccaatt tggcccccaa atcgcgtgga tgtcaatgga tgaaaaatga ccaacacgga
      481 aagaccacaa aataaatatg gttattgtgg aaaaataaat tacctttgaa ttacaaacgg
      541 atatggacga atttggacgg aaatggacac aaaaaaagcc acccttaggt agctaattat
      601 tgcatcactt ggtgccgaag gccggactcg aaccggcacg tatttctacg gttgattttg
      661 aatcaactgc gtctaccgat ttcgccactt cggcactgaa gaggtatgcg gaaaacgttg
      721 tggattatac ctgtcgcacg ccaccatgca agcgccagct tgctcgcacc tcgctaagtg
      781 ctgaaatttt cagcattgca taccgcgatg tgtaaccttt gtcacactcc aggcgccccg
      841 ccctgccatg ctctacactt cccagaccac accagagaag gataaaaaca tgtcgatgat
      901 aaaaagttat gccgcaaaag aggctggcgg cgaactggaa gtttatgagt acgatcccgg
      961 tgaactgaag ccacaagatg ttgaagttca ggtggattac tgcgggatct gccattccga
     1021 tctgtcgatg atcgacaacg aatggggatt ttcacagtat ccgctggttg ccgggcatga
     1081 ggtgattggg cgcgtggttg cattgggtag cgccgcgcag gataaaggtt tgcaggtcgg
     1141 tcagcgtgtc gggattggct ggacggcgcg tagctgtggt cactgcgacg cctgtattag
     1201 cggtaatcag atcaactgcg agcaaggtgc ggtgccgacg attttgaatc gcggcggttt
     1261 tgccgagaag ttgcgtgcgg actggcaatg ggtaatccca ctgccagaaa atatcgacat
     1321 tgaatctgct gggccgctgt tgtgcggcgg tatcacggtc tttaaaccac tgttgatgca
     1381 ccatatcact gccaccagcc gcgtcggcgt gattggtatt ggcgggctgg ggcatatcgc
     1441 tataaaactt ctgcacgcaa tgggatgcga ggtgacagcc tttagttcta atccggcgaa
     1501 agagcaggaa gtactggcga tgggtgccga taaagtggtg aatagccgcg atccgcaggc
     1561 actcaaagca ctggcggggc agtttgatct cattatcaac accgtcaacg tcagcctcga
     1621 ctggcagcct tattttgagg cgctgacgta tggcggtaat ttccatacgg tcggtgcggt
     1681 tctcacgccg ctgtctgttc cggcctttac gttaattgcg ggcgaccgca gcgtctctgg
     1741 ttctgctacc ggcacgcctt atgagctgcg taagctgatg cgctttgccg cccgcagcaa
     1801 ggttgcgccg actaccgaac tgttcccgat gtcgaaaatt aacgacgcta tccagcatgt
     1861 acgcgatggt aaagcgcgct accgcgtggt tctgaaagcc gacttctgat ccctttgccg
     1921 ggtgcctgtc gcccggcgtt taaatctctt tggaaggcgt ctcgcaatct gctgaattta
     1981 gcggtgaaga gtgcgcagcc gttgcctata ctccaggcag gaaactggag gatatctcat
     2041 gagcgaaccc ctgttaattg cccgcacgtc ggacacagaa ctgtttttat tgccgggaat
     2101 ggcgaatcgt cacgggctga ttactggcgc aacggggacg ggtaaaaccg ttacgctgca
     2161 aaaactggca gagtctctat ctgaaatcgg cgtgccggtg tttatggccg atgtgaaagg
     2221 tgatttaacg ggagtagcgg aggaaggtac ttcttcggaa aaactgctcg caaggcttaa
     2281 aaatatcggc gtaaatgact ggcaacctca tgctaatccg gtggtggtgt gggatatctt
     2341 tggcgagaaa ggccacccgg tgcgggcgac ggtttcggat ctggggccgc tgttgctggc
     2401 gcgactgttg aatctcaacg atgtgcaatc tggcgtgctg aatattatct tccgcattgc
     2461 ggacgatcag ggattgttgc tgctcgactt taaagatttg cgggcgatta cccagtacat
     2521 cggcgataac gccaaatcct tccagaatca gtacggaaat atcagtagcg catcggttgg
     2581 tgccatccag cgcggattac tgtcgctgga acagcaaggc gcagcacact tctttggtga
     2641 gccgatgctg gatatcaaag actggatgcg caccgatgcc aacggtaaag gcgttatcaa
     2701 tatcctcagc gccgagaaac tttatcagat gccgaaactg tacgccgcca gcctgccgtg
     2761 gatgctttca gagttatatg aacaattgcc tgaagcgggc gatctggaga aaccaaaact
     2821 ggtgtttttc ttcgacgaag cacatctgct gtttaacgat gcgcctcagg tactgctgga
     2881 taagattgag caggtgataa ggcttattcg ctcaaaaggc gtgggcgtct ggttcgtttc
     2941 gcaaaacccg tctgatattc cggataatgt gctcgggcag ctcggtaatc gtgttcaaca
     3001 cgctttgcgg gcttttacgc ccaaagatca gaaagcagta aaagctgcgg cgcaaaccat
     3061 gcgggccaat ccggcatttg ataccgaaaa ggcaatccag gaactgggga ccggcgaggc
     3121 gttaatctcg tttctcgatg caaaaggaag tccttctgtg gtggaacggg cgatggtgat
     3181 cgcaccttgt tcgcgaatgg ggccggtgac ggaagatgag cgtaatggcc tgattaatca
     3241 ctccccggtg tatggcaaat atgaagatga ggtggaccgc gagtccgcct atgagatgct
     3301 gcaaaaaggc tttcaggcca gtaccgagca gcaaaataat ccccccgtga aagggaaaga
     3361 ggtggcggtg gatgacggta ttcttggtgg attgaaggat attttgtttg gcactaccgg
     3421 accacgcggc gggaagaaag atggtgtggt gcaaacaatg gcgaaaagcg ccgctcgcca
     3481 ggtgacgaat cagattgtac gcgggatgtt ggggagtttg ctggggggga gaaaaagata
     3541 agtcagacgt gatctgtgcc cctcacccta accctctccc caaaggggcg aggggaccgt
     3601 tcgaactcgt atttgacttt gtcaacaata ataaagggag ctttcgctcc cttgttagat
     3661 tacgattttc tcattaacag ccacaggctg attaagaaga agctggcgct tggcaacagt
     3721 gcgccgatga tcggcgggat gccataaacc aacgtcagcg ggccgaagat ctggtccagt
     3781 acgtagaaga caaaaccgaa gctgataccg gtgaccacac gcacgcccat cggtacgcta
     3841 cgcagtgggc caaagatgaa cgacagcgcc atcagcatca tcaccgccac ggatagcggc
     3901 tggaagattt tgctccacat gttgagctga taacgtccgg catcctgacc gctcgacttc
     3961 agatacttca cgtagttgtg caaaccactg atggagagtg catccgggtc cagcgccacc
     4021 acgcccagtt tgtctggcgt gaggttggtt ttccaggtgc cgctcaccgt ctgcgaaccg
     4081 gtaatctgtt tcggattggt caggtcagat tcatcaacct gcgacagacg ccagacttta
     4141 tgttccgggt caaacttcgc ggtagcggca taacgtacgg attgcagacg acgattctcg
     4201 ttaaaggcat aaatactgat gccacctaac tcctcgtcac ctttaacccg ctcaatgtag
     4261 acgaagttgt tgccatcttt cgcccataag ccttgctggg tagagagcaa cgagccgccg
     4321 tacatcgcct gcgcacggta gttacgcgcc atctgctcgc cctgcggcgc gacccattcg
     4381 ccaatcgcca tcgtcagcaa gaccagtgga atggcggttt tcatcaccga cagcgctacc
     4441 tgcatacggg taaaaccaga agcctgcatc accaccagtt cgctgcgctg cgccagcatc
     4501 ccaagaccaa gcaacgcccc aagcagagcc gccatcggga agaagatttg cacatctttc
     4561 ggcacgctca gcaaggtata cattcctgcg cctaacgcgt cgtaactccc ctgcccggct
     4621 tttttcagct gatcgacaaa cttgataatg cccgacagcg acaccagcat gaacagcgtc
     4681 atcatgatgg tggtgaaaat agttttaccg atatagcggt caagtacgcc aaaaggttgc
     4741 atcacaccgc tcctttacgc gaaaaactgg cgcgcaggcg gcggaccggc acggtgtccc
     4801 aaaggttgag aacaatcgcc aaagccagat aaatcaggtt aacggtccac atccacagcg
     4861 tcgggtccag cttaccttta ccgccgttcg atttcaggga ggtctggatc aggaagaaaa
     4921 gtagatacag cagcatggct ggcagcatcg acagtacgcg tccctgacgc gggttaacca
     4981 cgctcagcgg tacgaccata agtgccatca taaacacggt gaataccaac gtgatacgcc
     5041 agttcagttc tgcacgggca cgatcggtgt cagtgttcca caatgtgcgc atgtccatct
     5101 ggtcggtatc gttcgggtcg agcgccaccg cctggtgacc aatgatcgcc tgataatcct
     5161 ggaagtccgt aatgcggaaa tcacgtaaca gtgcagtgcc ttcgaagcgc gttccctggt
     5221 tgagagtgac gacctgggag ccgtcgcgca gctgggttaa atgtccggaa tcggccacca
     5281 ccacagaagg acgtgcatta ccttttggtc gaatttgcgc gaggaacaca tctttgaaat
     5341 cgctgccgtc aacgctttcg atgaacagca ccgagctgcc attagtcgct tgctggaatt
     5401 gcccttgcgc cagcgccgcc atgccagggt tcgctttcgc ttctgctaac acttcatcct
     5461 gatgacgcga tgaccacggt cccgcccaca tcacgttaac cgccgcgacg attgccgtga
     5521 ataccgcaag gatcattgcc gctttcacca gaaccgcttt gctcaggccg caggcatgca
     5581 ttaccgtaat ttcactttcg gtatacagtt tgcccagcgt catcagcagc ccgaggaaca
     5641 ggcttaatgg caggataagc tgcgccattt ccggcacgcc caacccgaga agggagagca
     5701 ccagattcgc cggaatatcg ccgtcaaccg ctgcgccgag gatcctcact aacttttgac
     5761 agaagaagat caaaagcaag atgaagagta tcgccagctg gcttttgagc gtctcccgca
     5821 ccagatatct tatgattatc actttaaata cgcccgtaaa aactcgtctt ttgcaggatt
     5881 ttagcttgtt tcatggctta aacgtcattt attctcttga gtcgtcgaaa tcgtcgctaa
     5941 gataattata ctcaacggat tcacctctca gattttgttc tgacgtgcca atgccgtaat
     6001 aacgttaaga ttaacacgaa gtcatcgcaa cagcggacat gagttacgaa agcttgcaat
     6061 tctatctgta gccaccgccg ttgtctttaa gattcaggag cgtagtgcat ggagtttagt
     6121 gtaaaaagcg gtagcccgga gaaacagcgg agtgcctgca tcgtcgtggg cgtcttcgaa
     6181 ccacgtcgcc tttctccgat tgcagaacag ctcgataaaa tcagcgatgg gtacatcagc
     6241 gccctgctac gtcggggcga actggaagga aaaccggggc agacactgtt gctgcaccat
     6301 gttccgaatg tactttccga gcgaattctc cttattggtt gcggcaaaga acgtgagctg
     6361 gatgagcgtc agtacaagca ggttattcag aaaaccatta atacgctgaa tgatactggc
     6421 tcgatggaag cggtctgctt tctgactgaa ctgcacgtta aaggccgtaa caactactgg
     6481 aaagtgcgtc aggctgtcga gacagcaaaa gagacgctct acagtttcga tcagttgaaa
     6541 acgaataaga gcgaaccgcg tcgcccgctg cgtaagatgg tgttcaacgt gccgacccgc
     6601 cgtgaactga ccagcggtga gcgcgcgatc cagcacggtc tggcgattgc cgccgggata
     6661 aaagcagcga aagatctcgg taatatgccg ccgaatatct gtaacgccgc ttatctcgcc
     6721 tctcaggcgc gccagctggc tgacagctac agcaagaatg tcatcacccg cgttatcggc
     6781 gaacagcaga tgaaagagct gggaatgcac tcttatctgg cggtcggtca gggttcacaa
     6841 aacgaatcgc tgatgtcggt gattgagtac agaggcaacg cgtcggaaga tgcacgccca
     6901 atcgtgctgg tgggtaaagg tttaaccttc gactccggcg gtatctccat caagccttca
     6961 gaaggcatgg atgagatgaa gtacgatatg tgcggcgcgg cggcggttta cggcgtgatg
     7021 cgtatggtcg cggagctgca actgccgatt aacgttattg gcgtgctggc aggctgtgaa
     7081 aacatgcctg gcgggcgtgc ctatcgtccg ggcgatgtgt taaccaccat gtccggtcaa
     7141 accgttgaag tgctgaacac cgatgccgaa ggccgcctgg tactgtgcga cgtgttaact
     7201 tacgttgaac gttttgagcc ggaagcagtg attgacgtgg cgacgctgac cggtgcctgc
     7261 gtgatcgcgc tgggtcatca tattactggt ctgatggcga accataatcc gctggcccat
     7321 gaactgattg ccgcgtctga acaatccggt gaccgcgcat ggcgcttacc gctgggtgac
     7381 gagtatcagg aacaactgga gtccaatttt gccgatatgg cgaacattgg cggtcgtcct
     7441 ggtggggcga ttaccgcagg ttgcttcctg tcacgcttta cccgtaagta taactgggct
     7501 cacctggata ttgcaggaac cgcctggcgt tctggtaaag caaaaggcgc aaccggtcgt
     7561 ccggtagcgt tgctggcaca gttcctgtta aaccgcgctg ggtttaacgg cgaagagtaa
     7621 gtttgcgttt taccctcacc ctaaccctct ccctaaggga gagggaaccg atcgagcgca
     7681 atagaaattc attgctcatc tttcagggtg aggaatgcag caatatttaa atccaccaca
     7741 agaagcccca tatatgaaaa acgcaacgtt ctaccttctg gacaatgaca ccaccgtcga
     7801 tggcttaagc gccgttgagc aactggtgtg tgaaattgcc gcagaacgtt ggcgcagcgg
     7861 taagcgcgta ctcatcgcct gtgaagatga aaagcaggct taccggctgg atgaagccct
     7921 gtgggcgcgt ccggcagaaa gctttgttcc gcataattta gcgggagaag gaccgcgcgg
     7981 cggtgcgccg gtggagatcg cctggccgca aaagcgtagc agcagcccgc gcgatatatt
     8041 gattagtctg cgaacaagct ttgcagattt tgccaccgct ttcacagaag tggtagactt
     8101 cgttccttat gaagattctc tgaaacaact ggcgcgcgaa cgctataaag cctaccgcgt
     8161 ggctggtttc aacctgaata cggcaacctg gaaataatgg aaaagacata taacccacaa
     8221 gatatcgaac agccgcttta cgagcactgg gaaaagcagg gctactttaa gcctaatggc
     8281 gatgaaagcc aggaaagttt ctgcatcatg atcccgccgc cgaacgtcac cggcagtttg
     8341 catatgggtc acgccttcca gcaaaccatc atggatacca tgatccgcta tcagcgcatg
     8401 cagggcaaaa acaccctgtg gcaggtcggt actgaccacg ccgggatcgc tacccagatg
     8461 gtcgttgagc gcaagattgc cgcagaagaa ggtaaaaccc gtcacgacta cggccgcgaa
     8521 gctttcatcg acaaaatctg ggaatggaaa gcggaatctg gcggcaccat tacccgtcag
     8581 atgcgccgtc tcggcaactc cgtagactgg gagcgtgaac gcttcactat ggacgaaggc
     8641 ctgtccaatg cggtgaaaga agttttcgtt cgtctgtata aagaagacct gatttaccgt
     8701 ggcaaacgcc tggtaaactg ggacccgaaa ctgcgtaccg ctatctctga cctggaagtg
     8761 gaaaaccgcg aatcgaaagg ttcgatgtgg cacatccgct atccgctggc tgacggcgcg
     8821 aaaaccgcag atggtaaaga ttatctggtg gttgcgacta cccgtccgga aaccctgctg
     8881 ggcgataccg gcgtggccgt taacccggaa gatccgcgtt acaaagatct gattggcaaa
     8941 tatgtcattc tgccgctggt taaccgtcgt attccgatcg ttggcgacga acacgccgac
     9001 atggaaaaag gcaccggctg cgtgaagatc accccggcgc acgactttaa cgactatgaa
     9061 gtgggtaaac gtcacgccct gccgatgatc aacatcctga cctttgacgg tgatatccgt
     9121 gaaagcgccc aggtgttcga taccaaaggt aacgaatctg acgtttattc cagcgaaatc
     9181 ccggcagagt tccagaaact cgagcgtttt gccgcacgta aagcggtcgt tgctgcggtt
     9241 gacgcactcg gcctgctgga agaaattaaa ccgcacgacc tgaccgttcc ttacggcgac
     9301 cgtggcggcg tagttatcga accgatgctg accgaccagt ggtacgtgcg tgccgatgtc
     9361 ctggcgaaac cggcggttga agcggttgag aacggcgaca ttcagttcgt accgaagcag
     9421 tacgaaaaca tgtacttctc ctggatgcgc gatattcagg actggtgtat ctctcgtcag
     9481 ctgtggtggg gtcaccgtat cccggcatgg tacgacgaag cgggtaacgt ttatgttggc
     9541 cgcaacgaag acgaagtgcg taaagaaaat aacctgagcg cggacgtcgc gctgcgtcag
     9601 gacgaagacg ttctcgacac ctggttctcc tccgcgctgt ggaccttctc cacgctcggc
     9661 tggccggaaa acaccgacgc actgcgccag ttccacccaa ccagcgtgat ggtatccggc
     9721 ttcgacatca tcttcttctg gattgcccgc atgatcatga tgaccatgca cttcatcaaa
     9781 gatgaaaatg gcaaaccgca ggtgccgttc cacaccgttt acatgaccgg cctgattcgt
     9841 gatgacgaag gccagaagat gtccaaatcc aagggtaacg tgattgaccc gctggatatg
     9901 gttgacggta tttcgctacc ggaactgctg gaaaaacgta ccggcaatat gatgcagccg
     9961 cagctggcgg acaaaatccg taagcgcacc gagaagcagt tcccgaacgg cattgagccg
    10021 cacggtactg acgcgctgcg cttcaccctg gcggcgctgg cttctaccgg tcgtgacatc
    10081 aactgggata tgaagcgtct ggaaggctac cgtaacttct gtaacaagct gtggaacgcc
    10141 agccgctttg tgctgatgaa cacagaaggt caggattgcg gcttcaacgg cggcgaaatg
    10201 acgctatcgc tggcggaccg ctggattctg gcggagttca accagaccat caaagcgtac
    10261 cgcgaagcgc tggacagctt ccgcttcgat atcgccgcag gcattttgta tgagttcacc
    10321 tggaaccagt tctgtgactg gtatctggag ctgaccaagc cggtaatgaa cggtggaaca
    10381 gaagccgaac tgcgcggtac tcgccatacg ctggtgactg tactggaagg tctgctgcgc
    10441 ctcgcgcatc cgatcattcc gttcatcacc gaaaccatct ggcagcgtgt gaaagtactt
    10501 tgcggtatca ctgccgacac catcatgctg cagccattcc cgcagtatga tgcatctcag
    10561 gttgatgaag ccgcactggc cgacaccgag tggctgaaac aggcgatcgt tgctgtacgt
    10621 aacatccgtg cagaaatgaa catcgcgccg ggcaaaccgc tggagctgtt gcttcgtggt
    10681 tgcagcgcgg atgcagagcg tcgcgtaaat gaaaaccgtg gcttcctgca aacgctggcg
    10741 cgtctggaaa gcatcaccgt gctgcctgcc gatgacaaag gtccggtttc cgttaccaag
    10801 atcatcgacg gcgcagagct gctgatcccg atggctggcc tcatcaacaa agaagatgag
    10861 ctggcgcgcc tggcgaaaga agtggcgaag atcgaaggtg aaatcagccg catcgagaac
    10921 aagctggcaa atgaaggctt tgtcgcccgc gcaccggaag cggttatcgc gaaagagcgt
    10981 gagaagctgg aaggttatgc agaagcgaaa gccaagctga ttgaacagca ggctgttatc
    11041 gccgcactgt aatcaacaca gtgaagaaaa cgaaggccgg agcatgctcc ggcctttttt
    11101 atctcttaca gaaatggtaa agaaggcata actccccgag aaatactggc taagaagcct
    11161 ttatctggtt gttcttcttt attgattaaa ggaaggtcat ccaaatcgcc cagcaaatac
    11221 gtattttttg cctgccagtc aatcatccat attttcagca gtggataagc cagaccgccc
    11281 gtaatcccgg atatcaccac caacgcacac atgcgataaa gcataccgtg gtacgttaaa
    11341 gttgagcgaa aacgaatacg cccatcattc agtgacaggt tgctcataaa atgatttcgc
    11401 aacgacaccg ttaaataact ggtgctaaca gcaatcccaa aataatagat taactgcgcg
    11461 attatcattt tacgttgcat ttccataaat tgctgtaaat tctcaatgtc atcatttgca
    11521 tatacagaac tatcatacgc atttaatatt tgatcgaaga taatataacc agcaacagcg
    11581 agaaaaggca ataaagctaa aaatgcgaga atggcatatt ttatacaata cgtagtatct
    11641 aatttcacct tgaaacgatg tataccgaaa ctggtattgc tccagagaaa gctcattacc
    11701 aggctatata aagtaccatt aaaaatacca atggaaacaa taccaactat tgccatcaga
    11761 accacagata taataacact acttgaacta ttggcaggta gcatctttgt cgagataaag
    11821 aaaacagtcc ccatcccaat ggccattaaa atcggcaaga aaaaggttac ccaccagaac
    11881 cctttcaaag agcaattaaa actaaatctt acgccgttga gactggtcat taaggcctga
    11941 taacgcagtc cttttgctgc cataaatata agaaaaacca acagtaacaa agtcaaaaca
    12001 cagccaacaa gcggcatatc tgctgacact gtcattaaga ttgcgaaata gaaaaaaaca
    12061 aaaacaagac aactaacaaa aacattccca ccggtaattc cataagaaaa tcgttgtccg
    12121 ttaacttcca tattagcata aagataacgc ttacatttca ttaatgccca tggtaaatag
    12181 atacctagcg ttataatcgt taacaacaca ttcaccacac aaataagaaa atatctctca
    12241 cctgtaccat gaaaaacaaa agaatgggac ggaacatcca tttcattaat aacttgagcc
    12301 ataaaaattc cctgaatt
//
LOCUS       DAZFLD010000141        12230 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.142, whole genome shotgun sequence.
ACCESSION   DAZFLD010000141 DAZFLD010000000
VERSION     DAZFLD010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12230
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.142"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..411)
                     /gene="yhaJ"
                     /locus_tag="ACHKBL_003401"
     CDS             complement(<1..411)
                     /gene="yhaJ"
                     /locus_tag="ACHKBL_003401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YhaJ"
                     /protein_id="HFO5820227.1"
                     /translation="MAKERALTLEALRVMDAIDRRGSFAAAADELGRVPSALSYTMQK
                     LEEELDVVLFDRSGHRTKFTNVGRMLLERGRVLLEAADKLTTDAEALARGWETHLTIV
                     TEALVPTPAFFPLIDKLAAKANTQLAIITEVLAGA"
     gene            516..1217
                     /gene="yhaK"
                     /locus_tag="ACHKBL_003402"
     CDS             516..1217
                     /gene="yhaK"
                     /locus_tag="ACHKBL_003402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HFO5820228.1"
                     /translation="MITTRTARQCGQADYGWLQARYTFSFGHYFDPKLLGYASLRVLN
                     QEVLAPGAAFQPRTYPKVDILNVILDGEAEYRDSEGNHVQASAGEALLLSTQPGVSYS
                     EHNLSKDKPLTRMQLWLDACPQRENPLIQKLALNMGKQQLIASPEGTVGSLQLRQQVW
                     LHHIVLDKGESANFQLHGPRAYLQSIHGKFHALTHHEEKAALTCGDGAFIRDEANITL
                     VADSPLRALLIDLPV"
     gene            1240..1404
                     /gene="yhaL"
                     /locus_tag="ACHKBL_003403"
     CDS             1240..1404
                     /gene="yhaL"
                     /locus_tag="ACHKBL_003403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhaL"
                     /protein_id="HFO5820229.1"
                     /translation="MSKKSAKKRQPVKPVVAKEPARTAKNFGYEEMLSELEAIVADAE
                     TRLAEDEATA"
     gene            complement(1536..2846)
                     /gene="cyuA"
                     /locus_tag="ACHKBL_003404"
     CDS             complement(1536..2846)
                     /gene="cyuA"
                     /locus_tag="ACHKBL_003404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfidase"
                     /protein_id="HFO5820230.1"
                     /translation="MFDSTLNPLWQRYILAVQEEVKPALGCTEPISLALAAAVAAAEL
                     EGPVERVEAWVSPNLMKNGLGVTVPGTGMVGLPIAAALGALGGNANAGLEVLKDATAQ
                     AIADAKALLAAGKVSVKIQEPCDEILFSRAKVWNGEKWACVTIVGGHTNIVHIETHNG
                     VVFTQQACVTEGEQESPLTVLSRTTLAEILKFVNEVPFAAIRFILDSAKLNCALSQEG
                     LSGKWGLHIGATLEKQCARGLLAKDLSSSIVIRTSAASDARMGGATLPAMSNSGSGNQ
                     GITATMPVVVVAEHFGADDERLARALMLSHLSAIYIHNQLPRLSALCAATTAAMGAAA
                     GMAWLVDGRYEIISMAISSMIGDVSGMICDGASNSCAMKVSTSASAAWKAVLMALDDT
                     AVTGNEGIVAHDVEQSIANLCALASHSMQQTDRQIIEIMASKAR"
     gene            complement(2874..4205)
                     /gene="cyuP"
                     /locus_tag="ACHKBL_003405"
     CDS             complement(2874..4205)
                     /gene="cyuP"
                     /locus_tag="ACHKBL_003405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026203.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFO5820231.1"
                     /translation="MEIASNKGVIADASTPAGRAGMSESEWREAIKFDSTDTGWVIMS
                     IGMAIGAGIVFLPVQVGLMGLWVFLLSSVIGYPAMYLFQRLFINTLAESPECKDYPSV
                     ISGYLGKNWGILLGALYFVMLVIWMFVYSTAITNDSASYLHTFGVTEGVLSDSPFYGL
                     VLICILVAISSRGEKLLFKISTGMVLTKLLVVAALGVSMVGMWHLYNVGSLPPLGLLV
                     KNAIITLPFTLTSILFIQTLSPMVISYRSREKSIEVARHKALRAMNIAFGILFVTVFF
                     YAVSFTLAMGHDEAVKAYEQNISALAIAAQFISGDGAAWVKVVSVILNIFAVMTAFFG
                     VYLGFREATQGIVMNILRRKMPAEKINENLVQRGIMIFAILLAWSAIVLNAPVLSFTS
                     ICSPIFGMVGCLIPAWLVYKVPALHKYKGMSLYLIIVTGLLLCVSPFLAFS"
     gene            complement(4480..5844)
                     /gene="tdcG"
                     /locus_tag="ACHKBL_003406"
     CDS             complement(4480..5844)
                     /gene="tdcG"
                     /locus_tag="ACHKBL_003406"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HFO5820232.1"
                     /translation="MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSH
                     IVVDLYGSLSLTGKGHATDVAIIMGLAGNSPQDVVIDEIPAFIELVTRSGRLPVASGA
                     HIVDFPVAKNIIFHPEMLPRHENGMRITAWKGQEELLSKTYYSVGGGFIVEEEHFGLS
                     HDVETPVPYDFHSAGELLKMCDYNGLSISGLMMHNELALRSKAEIDAGFARIWQVMHD
                     GIERGMNTEGVLPGPLNVPRRAVALRRQLVSSDNISNDPMNVIDWINMYALAVSEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFRRPVNERSIARYFLAAGAIGALYKMNASISG
                     AEVGCQGEIGVACSMAAAGLTELLGGSPAQVCNAAEIAMEHNLGLTCDPVAGQVQIPC
                     IERNAINAVKAVNAARMAMRRTSAPRVSLDKVIETMYETGKDMNDKYRETSRGGLAIK
                     VVCG"
     gene            complement(5916..6305)
                     /gene="tdcF"
                     /locus_tag="ACHKBL_003407"
     CDS             complement(5916..6305)
                     /gene="tdcF"
                     /locus_tag="ACHKBL_003407"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enamine/imine deaminase"
                     /protein_id="HFO5820233.1"
                     /translation="MKKIIETQRAPGAIGPYVQGVDLGSMVFTSGQIPVCPQTGEIPA
                     DVQDQARLSLENVKAIVVAAGLSVGDIIKMTVFITDLNDFATINEVYKQFFDEHQATY
                     PTRSCVQVARLPKDVKLEIEAIAVRSA"
     gene            complement(6319..8613)
                     /gene="tdcE"
                     /locus_tag="ACHKBL_003408"
     CDS             complement(6319..8613)
                     /gene="tdcE"
                     /locus_tag="ACHKBL_003408"
                     /EC_number="2.3.1.-"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-ketobutyrate formate-lyase/pyruvate
                     formate-lyase"
                     /protein_id="HFO5820234.1"
                     /translation="MKVDIDTSDKLYADAWLGFKGTDWKNEINVRDFIQHNYTPYEGD
                     ESFLAEATPATTELWEKVMEGIRIENATHAPLDFDTNIATTITAHDAGYIDQPLEKIV
                     GLQTDAPLKRALHPFGGINMIRSSFHAYGREMDSEFEYIFTDLRKTHNQGVFDVYSPD
                     MLRCRKSGVLTGLPDGYGRGRIIGDYRRVALYGISYLVRERELQFADLQSRLEKGEDL
                     EATIRLREELAEHRRALLQIQEMAAKYGFDISRPAQNAQEAVQWLYFAYLAAVKSQNG
                     GAMSLGRTASFLDIYIERDFKAGVLNEQQAQELIDHFIMKIRMVRFLRTPEFDSLFSG
                     DPIWATEVIGGMGLDGRTLVTKNSFRYLHTLHTMGPAPEPNLTILWSEALPIAFKKYA
                     AQVSIVTSSLQYENDDLMRTDFNSDDYAIACCVSPMVIGKQMQFFGARANLAKTLLYA
                     INGGMDEKLKIQVGPKTAPLMDDVLDYDKVMDSLDHFMDWLAVQYISALNIIHYMHDK
                     YSYEASLMALHDRDVYRTMACGIAGLSVATDSLSAIKYARVKPIRDENGLAVDFEIDG
                     EYPQYGNNDERVDSIACDLVERFMKKIKALPTYRNAVPTQSILTITSNVVYGQKTGNT
                     PDGRRAGTPFAPGANPMHGRDRKGAVASLTSVAKLPFTYAKDGISYTFSIVPAALGKE
                     DPVRKTNLVGLLDGYFHHEADVEGGQHLNVNVMNREMLLDAIEHPEKYPNLTIRVSGY
                     AVRFNALTREQQQDVISRTFTQAL"
     gene            complement(8647..9855)
                     /gene="tdcD"
                     /locus_tag="ACHKBL_003409"
     CDS             complement(8647..9855)
                     /gene="tdcD"
                     /locus_tag="ACHKBL_003409"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006082 - organic acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="propionate kinase"
                     /protein_id="HFO5820235.1"
                     /translation="MNEFPVVLVINCGSSSIKFSVLNASDCEVLMSGIADGINSENAF
                     LSVNGGEPAPLAHHSYEGALKAIAFELEKRNLNDSVALIGHRIAHGGSIFTESAIITD
                     EVIDNIRRVSPLAPLHNYANLSGIESAQQLFPGVTQVAVFDTSFHQTMAPEAYLYGLP
                     WKYYEELGVRRYGFHGTSHRYVSQRAHSLLNLAEDDSGLVVAHLGNGASICAVRNGQS
                     VDTSMGMTPLEGLMMGTRSGDVDFGAMSWVASQTNQSLGDLERVVNKESGLLGISGLS
                     SDLRVLEKAWHEGHERAQLAIKTFVHRIARHIAGHAASLHRLDGIIFTGGIGENSSLI
                     RRLVMEHLAVLGVEIDTEMNNRSNSFGERIVSSENARVICAVIPTNEEKMIALDAIHL
                     GKVNAPAEFA"
     gene            complement(9881..11212)
                     /gene="tdcC"
                     /locus_tag="ACHKBL_003410"
     CDS             complement(9881..11212)
                     /gene="tdcC"
                     /locus_tag="ACHKBL_003410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter TdcC"
                     /protein_id="HFO5820236.1"
                     /translation="MSTSDSIVSSQTKQSSWRKSDTTWTLGLFGTAIGAGVLFFPIRA
                     GFGGLIPILLMLVLAYPIAFYCHRALARLCLSGSNPSGNITETVEEHFGKTGGVVITF
                     LYFFAICPLLWIYGVTITNTFMTFWENQLGFAPLNRGFVALFLLLLMAFVIWFGKDLM
                     VKVMSYLVWPFIASLVLISLSLIPYWNSAVIDQVDLGSLSLTGHDGILITVWLGISIM
                     VFSFNFSPIVSSFVVSKREEYEKDFGRDFTERKCSQIISRASMLMVAVVMFFAFSCLF
                     TLSPANMAEAKAQNIPVLSYLANHFASMTGTKTTFAITLEYAASIIALVAIFKSFFGH
                     YLGTLEGLNGLILKFGYKGDKTKVSLGKLNTLSMIFIMGSTWVVAYANPNILDLIEAM
                     GAPIIASLLCLLPMYAIRKAPSLAKYRGRLDNVFVTVIGLLTILNIVYKLF"
     gene            complement(11234..12223)
                     /gene="tdcB"
                     /locus_tag="ACHKBL_003411"
     CDS             complement(11234..12223)
                     /gene="tdcB"
                     /locus_tag="ACHKBL_003411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HFO5820237.1"
                     /translation="MHITYDLPVAIDDIIEAKQRLAGRIYKTGMPRSNYFSERCKGEI
                     FLKFENMQRTGSFKIRGAFNKLSSLTDAEKRKGVVACSAGNHAQGVSLSCAMLGIDGK
                     VVMPKGAPKSKVAATCDYSAEVVLHGDNFNDTIAKVSEIVEMEGRIFIPPYDDPKVIA
                     GQGTIGLEIMEDLYDVDNVIVPIGGGGLIAGIAVAIKSINPTIRVIGVQSENVHGMAA
                     SFHSGEITTHRTTGTLADGCDVSRPGNLTYEIVRELVDDIVLVSEDEIRNSMIALIQR
                     NKVVTEGAGALACAALLSGKLDQYIQNRKTVSIISGGNIDLSRVSQITGFVDA"
BASE COUNT         3128 a         3334 c         2925 g         2843 t
ORIGIN      
        1 tgcccccgcc agcacttcgg tgatgattgc cagttgggta ttcgcttttg ccgccagttt
       61 gtcaattaac gggaaaaagg caggtgtcgg caccagcgct tcggtcacaa tggtgagatg
      121 cgtttcccaa ccgcgcgcga gagcttccgc atcggtagtc agtttatctg cggcttccag
      181 caaaacgcgt cctcgctcca gcaacatcct cccgacattg gtgaatttgg tgcgatggcc
      241 cgagcggtca aacagcacca catccagctc ttcttccagt ttttgcatgg tgtagctaag
      301 tgcggaaggc acgcgtccca gctcatccgc cgccgccgca aaactgcccc ggcgatcgat
      361 cgcatccata acccgtagtg cttccagcgt taatgccctt tctttggcca tttcgttctc
      421 attcaggaaa tttgaacata ccgggcagaa tatctggcta acaatgtagc gtccagcccc
      481 ttaccataaa aggaagtaaa gagaggtcaa gaattatgat tactacccga actgccaggc
      541 agtgtggaca agcagactac ggatggttgc aggcccggta tactttttcc tttggacact
      601 acttcgaccc gaaattgtta ggctatgcct ccctgcgcgt gcttaaccag gaagtgctgg
      661 ccccaggtgc cgcctttcag ccgcgaacct atcccaaagt cgatatttta aatgtgattc
      721 tggatggaga agcagagtat cgcgacagcg aaggcaatca tgttcaggcc agcgctggtg
      781 aggcgttgct gctctctacc cagccgggtg tcagctatag cgaacacaat ctcagcaaag
      841 acaaaccgtt aacgcgaatg cagctttggc tggatgcctg cccgcagcga gagaatccgc
      901 tgattcaaaa actggcgctt aatatgggca agcagcaatt aatcgcctcg ccagagggca
      961 cagtgggtag cctgcaactg cgccagcaag tgtggctgca ccatatcgtg ctcgacaaag
     1021 gcgaaagtgc gaatttccag ttgcatggac cacgcgcgta tttgcaatcg attcacggga
     1081 aatttcatgc gcttacgcat catgaagaga aagcggcgct aacctgcggt gatggggcgt
     1141 ttattcgtga cgaggctaac attacgctgg ttgccgattc cccactgcgc gctttgctga
     1201 tagatttgcc tgtctagttg tttttacagg gagatgatga tgagtaaaaa atcggccaaa
     1261 aagcgccagc cagtgaagcc cgtggtggcg aaagaacctg ctcgtaccgc caaaaatttt
     1321 ggctatgaag agatgttgag cgaactggaa gccatcgtcg cggatgctga aacgcgttta
     1381 gccgaggatg aagctaccgc gtaaagacag gtgccggatg cggcgtaaac gccttatccg
     1441 gcctacaaaa cccaacaaca tcaacaaatt gtgcaattcg taggccagat aagacgcgcc
     1501 agcatcgcat ccggcacgat acccaaaact ggcgtttatc tggccttgct cgccataatc
     1561 tcgataatct gccgatccgt ttgctgcatc gaatggcttg ctaacgcaca caggttggca
     1621 atcgactgct caacatcatg cgccacgatc ccctcattgc cggtcacggc ggtatcatcc
     1681 agcgccatta acaccgcttt ccacgcagcc gaagcactgg tcgaaacctt catcgcgcag
     1741 ctgttcgacg ctccatcgca aatcatgccg ctgacatcgc cgatcatact gctgatcgcc
     1801 atcgaaatga tttcataacg cccatccacc agccatgcca tcccggcggc ggcccccatt
     1861 gctgcggtcg ttgcggcaca cagcgcagac aaacgcggta actggttatg gatgtaaatt
     1921 gcgctcaaat gcgaaagcat cagcgcacgc gccagccgtt catcatcggc cccgaagtgt
     1981 tctgctacca ccaccacggg cattgttgca gtgatcccct ggttacccga gccggagtta
     2041 ctcattgccg gaagcgtagc gccgcccata cgcgcatcgg atgccgcgct ggtacgaatc
     2101 acaatggatg aagagagatc tttcgccagc aaaccgcgtg cacactgttt ttccagcgtc
     2161 gcgccaatat gcagccccca cttaccgctc aaaccttcct gcgataacgc acaatttagc
     2221 ttcgcggaat cgagaataaa gcggatcgcc gcaaacggga cttcattgac gaacttcagg
     2281 atctcagcca gcgtcgttct tgaaagcacc gtcagcggcg actcttgctc gccctctgtc
     2341 acacacgcct gctgggtaaa caccacacca ttgtgcgtct caatatgcac aatgttggta
     2401 tgcccgccga cgatggtgac acacgcccac ttctcaccgt tccagacttt ggcgcgtgag
     2461 aagaggattt catcgcaagg ttcctggatc ttaactgaga ctttccccgc cgccagcagt
     2521 gctttggcat cggcaattgc ctgcgcagtt gcgtctttca gcacttccag cccggcgtta
     2581 gcatttccac ctaacgctcc cagcgccgcc gcaattggca gccccaccat tcccgtgccg
     2641 ggaacggtga cgcccagacc gttcttcatc agatttggcg aaacccaggc ttctacacgt
     2701 tcaaccggac cttccagttc tgccgcagca accgccgccg ccagcgccag tgaaatcggt
     2761 tcagtacatc ccagcgccgg ttttacttcc tcctgaacgg cgaggatgta acgctgccat
     2821 aacggattta aagtcgaatc aaacatatta aaaaccttaa aatttcaggt aaatcaagaa
     2881 aatgccagga acggagaaac acaaagcaac aaaccagtga cgataatcag gtacagagac
     2941 atccctttgt atttgtgcaa tgccggtact ttgtaaacca gccacgccgg gatcaggcac
     3001 cctaccatgc cgaaaatcgg gctacagata gaggtgaagc tcaacaccgg tgcgttcagt
     3061 acgatggcgc tccaggccag caaaatggcg aaaatcatga tgccgcgctg aacgagattt
     3121 tcgttaatct tctcggcagg catcttgcga cgcaggatgt tcattacgat cccttgcgtt
     3181 gcttcgcgaa agcctaaata gacgccaaag aatgcagtca ttacagcaaa gatattgaga
     3241 atgacgctga ccactttcac ccacgctgcg ccatcaccgc taataaactg tgcggcaatc
     3301 gccaacgcgg aaatattctg ctcgtaggct tttaccgctt cgtcatgtcc catcgccagc
     3361 gtgaacgaca cggcgtagaa aaagacggtg acaaacaaaa tgccaaacgc gatattcatt
     3421 gcccgcaatg ctttatgccg cgccacttca attgattttt cccgcgagcg ataagagatc
     3481 accatcgggc ttaacgtctg gataaacaga atcgacgtca gggtaaacgg cagcgtaata
     3541 atggcgtttt tcaccagcag ccccagcggc ggtagcgaac cgacgttata cagatgccac
     3601 attcccacca tcgacacgcc cagcgccgcg accaccagca gcttggtcag caccatgccg
     3661 gtcgaaattt taaataacaa tttctcgccg cgtgaggaga tcgccaccag aatgcaaatc
     3721 agtaccagac cataaaaggg actgtctgac agcacccctt ccgtcacgcc gaaggtatgc
     3781 aggtaggaag cactatcgtt ggtgatggcg gtggaataga cgaacatcca aatcaccagc
     3841 attacgaaat agagcgcacc taacaggatg ccccagtttt tacctaaata accgctgatg
     3901 acgctcgggt aatctttaca ctccggtgat tctgccagcg tattaataaa caaccgctga
     3961 aacagataca ttgccgggta accaatcacc gatgagagca aaaataccca caatcccatc
     4021 aaaccgacct gcaccgggag aaaaacaatc cccgcgccaa tcgccatccc gatactcata
     4081 atcacccagc cggtgtcagt actgtcgaat ttgatcgctt ctcgccactc gctctcactc
     4141 attccagcac gacccgccgg ggtcgaagcg tctgcaatga cgcctttatt cgatgcaatt
     4201 tccataattt ctcgctcaat attttgtagg gcttattatt tttttccgag ccgcatcaag
     4261 gcgatatgcg gtctacgttt ttgcaggcga gttattagaa taaagaaatg atacgcgcga
     4321 ggtcagagaa aatcttcaca atcaagactt tgaaataacg gagatggata agaattttct
     4381 actaaattaa tcgcaagaga atattcattc tctatttgcg gcagatcaca aaaaaaaggt
     4441 gcacatttgt gcacccaagg atgaaagctg acagcaatgt cagccacaga ccactttaat
     4501 ggccagtcct ccgcgtgacg tttcgcggta tttatcgttc atatctttgc cggtttcata
     4561 catcgtctcg atcactttat cgagtgaaac acgcggtgcc gaggtgcggc gcatcgccat
     4621 ccgcgcggcg tttactgctt tcacggcatt aatggcatta cgttcaatgc acgggatttg
     4681 tacctgtccg gcaaccggat cgcaggtcag cccaaggtta tgctccatcg cgatttccgc
     4741 cgcattgcat acctgcgccg gactgccgcc caggagttca gttaaccctg ccgccgccat
     4801 tgaacaggcc acgccaatct ccccctgaca gccgacctcc gcaccagaga tggaggcgtt
     4861 cattttatac agcgcgccaa tagcccccgc tgcaagaaaa tagcgggcaa ttgaccgctc
     4921 gtttaccgga cgacggaact tatcgtaata agccagcact gccggaataa tgccgcacgc
     4981 accgttagtc ggtgccgtta ccacgcgccc gccagctgcg ttttcttcac taaccgccag
     5041 cgcgtacata ttgatccagt cgatgacatt catcggatcg ttagagatgt tatcgctgga
     5101 aaccagttga cgacgcagcg ctacggcacg acgcggcaca ttgagcggac caggcagcac
     5161 gccttcagtg ttcatcccgc gttcaatacc gtcatgcatc acttgccaga tacgggcaaa
     5221 ccctgcgtca atttccgctt tactgcgcag cgccagctcg ttgtgcatca tcaggccaga
     5281 tatagacagg ccgttgtaat cacacatttt cagcagttca cctgctgagt ggaaatcgta
     5341 aggtacgggt gtttcgacat cgtgcgacag gccgaagtgt tcttcttcga caataaaccc
     5401 gccgccgaca gagtaatagg ttttgcttaa tagctcttcc tgtcccttcc aggcagtgat
     5461 ccgcattccg ttctcatggc gaggcaacat ttcgggatgg aagataatgt tctttgctac
     5521 aggaaaatca acaatatgcg cgccagaagc caccggcaac cgcccgctgc gcgtcactaa
     5581 ctctataaat gcagggatct catcaatgac aacatcctgc ggactgtttc ctgccagtcc
     5641 catgatgatg gcgacatccg tggcatggcc tttgcccgtc agtgacaacg acccgtacag
     5701 atcgaccaca atatggctcg tcgcggttaa taagccgcta ctttccagcc gatcaataaa
     5761 actttttccg gcattcattg gccccacagt atgcgaactg gagggaccaa tcccaatttt
     5821 gaaaatatcg aatgcactaa tcatatccac accctcggat tgccgttcag tgaagtggag
     5881 cggaacgacc ttgcgaccgt cccgctcacg aggccttacg cactacgtac tgcgatggct
     5941 tcaatttcca acttcacatc tttcggcaaa cgcgcgacct gcacacagct ccgggtcggg
     6001 taggtcgcct gatgttcatc gaagaactgc ttatagactt cattgatggt ggcaaaatca
     6061 ttcagatcgg tgatgaacac ggtcatcttg atgatatcgc ccacactcag cccggcagca
     6121 accacgatcg ctttgacgtt ttcgaggctt aaacgcgcct gatcttgcac atcagccggg
     6181 atctcaccgg tctgtgggca aaccggaatt tgcccggagg tgaagaccat gctgcctaaa
     6241 tcaacgccct gaacataagg gccgattgcg cctggggcac gttgcgtttc gataatcttt
     6301 ttcatacatc ctccggcgtc agagcgcctg ggtaaaggta cgtgaaataa catcctgttg
     6361 ctgttcacgg gtcagtgcgt tgaagcgcac ggcgtagcca gagacacgga ttgtcaggtt
     6421 aggatatttt tccgggtgct cgatggcatc cagcagcatt tcccgattca ttacgttgac
     6481 gttgaggtgt tgaccgcctt cgacatccgc ttcgtggtgg aaatacccat ccagcaggcc
     6541 gacaaggttg gttttacgta ctggatcttc tttgcccagc gccgcaggga cgatggagaa
     6601 ggtgtacgag atcccatctt tggcgtaggt gaacggcagt ttcgccaccg acgtcaatga
     6661 ggccacggcg cctttgcgat cacgaccgtg catcgggtta gcgcccggcg cgaacggtgt
     6721 tccggcgcgg cgaccgtccg gcgtgttccc ggttttctga ccgtacacca cgttagaggt
     6781 gatagtcaga atcgattgag tcggcacagc attgcgatag gttggcagag ctttaatttt
     6841 cttcataaag cgttcaacca gatcgcaggc aatgctgtct acgcgctcgt cgttgttgcc
     6901 atactgcgga tattcgccgt cgatttcgaa atccaccgcc aggccgtttt cgtcacggat
     6961 tggtttcaca cgagcatatt tgatggcaga cagggagtcc gttgccaccg acaggcccgc
     7021 gatgccgcac gccatggtgc gatagacatc acgatcgtgt agcgccatca gcgaggcttc
     7081 atagctgtac ttgtcgtgca tgtagtggat gatattcagc gcgctgatgt actgtaccgc
     7141 cagccagtcc atgaagtggt cgaggctatc catcactttg tcgtagtcca gcacgtcatc
     7201 catcaacgga gccgttttcg ggccaacctg aatcttcagc ttctcgtcca ttccgccgtt
     7261 gattgcgtac agtagcgttt tcgccaggtt agcgcgcgca ccgaagaact gcatctgctt
     7321 accaatcacc attgggctga cgcaacaggc aatcgcgtaa tcgtcgctat tgaagtcagt
     7381 acgcatcaga tcgtcgtttt catactgcaa agaggatgtg acgatagaca cctgcgcggc
     7441 atattttttg aaggcaatcg gcaaggcttc cgaccacaga atagtcaggt taggttccgg
     7501 tgcaggcccc atagtgtgca gggtgtgtaa atagcggaag gagtttttgg tcaccagggt
     7561 tcgaccgtcc agccccatcc cgccgatcac ttccgtcgcc cagatcgggt cgccggagaa
     7621 cagcgaatca aattccggcg tacgcaagaa gcgcaccata cggatcttca tgatgaagtg
     7681 gtcgatcagt tcctgtgcct gctgctcatt gagtacgcct gctttaaagt cgcgctcaat
     7741 gtagatatcg aggaacgatg ccgtgcgccc cagcgacatc gcgccgccat tttgcgattt
     7801 cactgccgcc aggtaagcga agtagagcca ctgcaccgct tcctgtgcat tctgcgccgg
     7861 acgggagatg tcaaaaccgt atttcgccgc catttcttga atctgcaaca gcgcacgacg
     7921 atgttccgcc agctcctcac gcagacggat cgtagcttcg agatcttcgc ctttttccag
     7981 acgggactgg agatcggcaa actgcagttc gcgttcacgt accagataac tgatgccgta
     8041 aagcgccacg cggcggtagt caccgatgat acgcccacga ccataaccat ctggtaaacc
     8101 ggtcagtaca ccagatttac ggcagcgcag catatccggt gagtaaacat caaatacgcc
     8161 ctggttatgg gttttacgca gatcggtaaa gatgtattca aattcgctat ccatttcacg
     8221 accatacgcg tggaatgaac ttctaatcat attaatgccg ccgaacggat gcagcgcacg
     8281 ctttaatggc gcatccgttt gcagacccac tattttttcc agcggctggt caatatatcc
     8341 tgcatcatga gcggtaattg tggtggcaat attggtatcg aaatccaatg gcgcgtgagt
     8401 cgcattttcg atacggatgc cttccatcac tttttcccac aattccgtgg tggcaggcgt
     8461 cgcttcggcg agaaaagatt catcaccttc atacggtgta tagttatgtt gaataaaatc
     8521 gcggacatta atttcgtttt tccagtccgt acctttaaag ccaagccatg cgtcggcgta
     8581 cagcttatcg ctggtatcaa tatctacctt catgaaaaat aatctctcta caatacttca
     8641 actaaattat gcaaattctg cgggcgcgtt cactttgcct aaatgaatgg catccaaagc
     8701 aatcattttt tcttcgttag tcggaattac ggcgcaaatg acacgcgcat tttcactgga
     8761 aacaattcgc tctccaaaag agttagagcg attattcatt tctgtatcaa tctctacgcc
     8821 taatacagct aaatgttcca tgaccagacg acgaattaaa cttgaattct ctcctattcc
     8881 gccggtgaat ataattccat ccaggcgatg taatgaagca gcgtgtccgg caatatgacg
     8941 ggcaattcga tgaacaaagg ttttaattgc caattgcgcg cgttcgtgac cttcatgcca
     9001 ggctttttcc agaacacgta aatccgaaga aaggccggaa atacctaata atcccgactc
     9061 tttattcacg acgcgttcca ggtcacccag gctctggttg gtttggctgg cgacccagga
     9121 catcgccccg aagtcgacat caccactgcg ggtacccatc atcaagcctt ccagcggcgt
     9181 cattcccatt gaggtatcaa cactctgacc gttgcgaact gcgcagattg acgcgccatt
     9241 gccaagatgc gccacaacca ggccggagtc atcttccgcc agattcagca aagaatgcgc
     9301 gcgctgggaa acatagcggt gcgacgtgcc gtggaaacca taacggcgta cacctaactc
     9361 ttcataatat ttccacggca ggccgtataa ataagcttcc ggagccatcg tctggtggaa
     9421 actggtatca aataccgcta cctgagttac gcccggaaat aattgctgcg ccgattcaat
     9481 accacttaaa ttggcgtaat tatgcagggg tgccagtgga gaaacgcgac ggatattatc
     9541 aatgacttca tcggtaataa tggcggactc ggtaaaaata ctgccgccgt gagcgatgcg
     9601 gtggccaatt aaggccacac tgtcatttaa attccgtttt tccagttcaa atgcaattgc
     9661 cttcaatgca ccttcgtagc tgtggtgagc cagcggtgct ggctctcccc catttacgga
     9721 taagaatgca ttttccgagt taataccgtc ggcaatacct gacattaata cttcacagtc
     9781 gctggcattg agtacggaaa acttaatcga agacgaacca cagttaataa ccaaaacaac
     9841 cggaaattca ttcatctctt ttctcatcct gagttacgga ttaaaacagt ttgtatacga
     9901 tgttcaggat agtcagcaga ccaatcacgg taacaaacac gttatccaga cgaccacggt
     9961 atttcgccag agacggcgct ttacgaatgg catacatcgg caacaggcat agcagtgatg
    10021 cgataatcgg tgcgcccatg gcttcaatca ggtcgaggat gttcgggttg gcgtaggcaa
    10081 caacccaggt ggagcccatg atgaagatca tgctgagagt attcagttta cccagcgaca
    10141 ctttggtttt gtcaccttta taaccgaact tcagaatcag gccattcaag ccttccagcg
    10201 tccccagata gtgaccgaag aaagatttga agatagccac gagtgcgatg atggaagccg
    10261 catattccag tgtaatcgcg aacgttgttt tggtaccggt catggacgca aagtggttag
    10321 ccagataaga aagcactgga atattctgcg ctttggcttc cgccatgttg gccggagaca
    10381 gcgtaaacag gcagctaaag gcaaagaaca tcaccactgc aaccatcagc atgctggcac
    10441 gagaaatgat ttgggaacat ttacgttcgg tgaagtcgcg accgaagtct ttctcatact
    10501 cttcacgttt agaaaccacg aaggaagaga cgattggcga gaagttaaag gagaaaacca
    10561 tgatggaaat ccccagccag acagtgatca ggataccgtc atgaccggtt aacgacagcg
    10621 aaccgaggtc aacctggtcg ataactgcag agttccagta agggatcagc gacagagaaa
    10681 tcagcaccag gctggcgata aacggccata ccaggtagct catcacttta accatcagat
    10741 ccttaccaaa ccagatgacg aaagccatca gcagcaacag gaacagcgcc acaaagccgc
    10801 gattcagcgg tgcaaagccg agctggtttt cccagaacgt cataaaggta ttggtaatag
    10861 taacgccata aatccacagc agtgggcaaa tcgcgaagaa gtacaggaac gtgataacca
    10921 cgccgccagt tttaccaaaa tgctcttcca ccgtttccgt aatgttgccg gaagggttag
    10981 agccagaaag acacagacgc gccagtgccc ggtggcaata aaacgcgatg gggtatgcta
    11041 ataccaacat cagaagaatc gggatcagtc cgccaaaacc tgcgcggata gggaagaaca
    11101 gcaccccggc gccgattgcc gtaccaaaca agcctaacgt ccatgtggta tctgatttac
    11161 gccaggacga ttgttttgtc tggctggata caatgctatc tgaagtactc atatcctatc
    11221 ctcaacgaat taattaagcg tcaacgaaac cggtgatttg agagacgcga gaaagatcga
    11281 tattgccgcc ggaaataata ctgacggttt ttctgttttg aatatattgg tctaatttac
    11341 cgcttaataa tgcagcacat gccagagcgc ctgcgccttc ggtgacgact ttattgcgct
    11401 gaattaaggc aatcatactg tttctgattt cgtcttcgct gaccagcacg atgtcatcga
    11461 ctaattcacg aacgatttcg taagttaaat tacccgggcg ggagacatca caaccatccg
    11521 ccagggtgcc ggtagttcgg tgcgtagtta tttctccgga gtggaaagaa gccgccatgc
    11581 cgtgaacgtt ttctgactgt acgccaataa cacgaatagt cgggttaatt gatttaatcg
    11641 ctaccgcaat accagcaatt aaaccgccac cgccaattgg cacaatcaca ttatcgacat
    11701 catagagatc ttccataatt tccagaccaa tcgttccctg gccagcaatc actttcggat
    11761 catcgtaagg tgggataaaa atacggcctt ccatttcgac aatttcgctc actttagcaa
    11821 tagtgtcgtt gaagttatca ccatgcaaaa cgacttctgc ggagtagtcg cacgttgccg
    11881 cgactttgga tttcggcgca ccttttggca tcaccacttt accgtcaata cccagcatcg
    11941 cacaggagag cgaaacccct tgcgcatggt tgcccgcaga gcaggccact acacctttac
    12001 gtttttccgc atcagtcagt gaacttaatt tattaaatgc gccacgaatt ttaaatgaac
    12061 cggtacgctg catgttttcg aatttgagga atatttcacc tttgcaacgt tcactaaaat
    12121 agttggagcg aggcatacct gttttataaa ttcgcccagc cagtcgttgt tttgcttcaa
    12181 tgatgtcatc aatagcaacc gggagatcgt atgtaatatg cattataaaa
//
LOCUS       DAZFLD010000142        11971 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.143, whole genome shotgun sequence.
ACCESSION   DAZFLD010000142 DAZFLD010000000
VERSION     DAZFLD010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11971)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11971)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11971
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.143"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            3..605
                     /gene="yebB"
                     /locus_tag="ACHKBL_003412"
     CDS             3..605
                     /gene="yebB"
                     /locus_tag="ACHKBL_003412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebB family permuted papain-like enzyme"
                     /protein_id="HFO5820238.1"
                     /translation="MNVNYPAEYEIGDIVFTCIGATLFGQISAASNCWSNHVGIIIGH
                     NGEDFLVAESRVPLSTITTLSRFIKRSSNQRYAIKRLDAGLTEQQKQRIVEQVPSRLR
                     KLYHTGFKYESSRQFCSKFVFDIYKEALCIPVGEIETFGELLNSNPNAKLTFWKFWFL
                     GSIPWERKTVTPASLWHHPGLVLIHAEGVETPQPELTEAV"
     gene            complement(607..1128)
                     /gene="ruvC"
                     /locus_tag="ACHKBL_003413"
     CDS             complement(607..1128)
                     /gene="ruvC"
                     /locus_tag="ACHKBL_003413"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HFO5820239.1"
                     /translation="MAIILGIDPGSRVTGYGVIRQVGRQLSYLGSGCIRTKVDDLPSR
                     LKLIYAGVTEIITQFQPDYFAIEQVFMAKNADSALKLGQARGVAIVAAVNQELPVFEY
                     AARQVKQTVVGIGSAEKSQVQHMVRTLLKLPANPQADAADALAIAITHCHVSQNAMQM
                     SESRLNLARGRLR"
     gene            complement(1163..1903)
                     /gene="yebC"
                     /locus_tag="ACHKBL_003414"
     CDS             complement(1163..1903)
                     /gene="yebC"
                     /locus_tag="ACHKBL_003414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFO5820240.1"
                     /translation="MAGHSKWANTRHRKAAQDAKRGKIFTKIIRELVTAAKLGGGDPD
                     ANPRLRAAIDKALSNNMTRDTLNRAIARGVGGDDDANMETIIYEGYGPGGTAIMIECL
                     SDNRNRTVAEVRHAFSKCGGNLGTDGSVAYLFSKKGVISFEKGDEDTIMEAALEAGAE
                     DVVTYDDGAIDVYTAWEEMGKVRDALEAAGLKADSAEVSMIPSTKADMDAETAPKLMR
                     LIDMLEDCDDVQEVYHNGEISDEVAATL"
     gene            complement(1932..2384)
                     /gene="nudB"
                     /locus_tag="ACHKBL_003415"
     CDS             complement(1932..2384)
                     /gene="nudB"
                     /locus_tag="ACHKBL_003415"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008828 - dATP diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0019177 - dihydroneopterin triphosphate
                     pyrophosphohydrolase activity [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HFO5820241.1"
                     /translation="MKDKVYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVE
                     EGETAPQAAMREVKEEVTIDVVAEQLTLIDCQRTVEFEIFSHLRHRYAPGVTRNTESW
                     FCLALPHERQIVFTEHLAYKWLDAPAAAALTKSWSNRQAIEQFVINAA"
     gene            complement(2502..4274)
                     /gene="aspS"
                     /locus_tag="ACHKBL_003416"
     CDS             complement(2502..4274)
                     /gene="aspS"
                     /locus_tag="ACHKBL_003416"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004815 - aspartate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006422 - aspartyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HFO5820242.1"
                     /translation="MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGI
                     VQVFFDPDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLTII
                     NRADVLPLDSNHVNTEEARLKYRYLDLRRPEMAQRLKTRAKITSLVRRFMDDHGFLDI
                     ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRD
                     EDLRADRQPEFTQIDVETSFMTAPQVREVMEALVRHLWLEVKGVDLGDFPVMTFAEAE
                     RRYGSDKPDLRNPMELTDVADLLKSVEFAVFAGPANDPKGRVAALRVPGGASLTRKQI
                     DEYGNFVKIYGAKGLAYIKVNERAKGLEGINSPVAKFLNAEIIEAILDRTAAQDGDMI
                     FFGADNKKIVADAMGALRLKVGKDLGLTDESKWAPLWVIDFPMFEDDGEGGLTAMHHP
                     FTSPKDMTAAELKAAPENAVANAYDMVINGYEVGGGSVRIHNGDMQQTVFGILGINEE
                     EQREKFGFLLDALKYGTPPHAGLAFGLDRLTMLLTGTDNIRDVIAFPKTTAAACLMTE
                     APSFANPTALAELSIQVVKKDENN"
     gene            4584..5150
                     /gene="yecD"
                     /locus_tag="ACHKBL_003417"
     CDS             4584..5150
                     /gene="yecD"
                     /locus_tag="ACHKBL_003417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFO5820243.1"
                     /translation="MLELNAKTTALVVIDLQEGILPFAGGPHTADEVVNRAGKLAAKF
                     RASGQPVFLVRVGWSADYAEALKQPVDAPSPAKVLPENWWQHPAALGATDSDIEIIKR
                     QWGAFYGTDLELQLRRRGIDTIVLCGISTNIGVESTARNAWELGFNLVIAEDACSAAS
                     AEQHNNSINHIYPRIARVRSVEEILHAL"
     gene            5147..5965
                     /gene="yecE"
                     /locus_tag="ACHKBL_003418"
     CDS             5147..5965
                     /gene="yecE"
                     /locus_tag="ACHKBL_003418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HFO5820244.1"
                     /translation="MIYIGLPQWSHPKWVRLGITNLEEYARHFNCVEGNTTLYALPKP
                     EVVLRWREQTTDDFRFCFKFPATISHQAALRHCDDLVTEFLTRMSPLAPRIGQYWLQL
                     PATFGPRELPALWHFLDSLPGEFNYGVEVRHPQFFAKGEEEQTLNRGLHQRGVNRVIL
                     DSRPVHAARPHSEAIRDAQRKKPKVPVHAVLTAKNPLIRFIGSDDMTQNRELFQVWLQ
                     KLAQWHQTTTPYLFLHTPDIAQAPELVHTLWEDLRKTLPEIGAVPAIPQQSSLF"
     gene            6018..6413
                     /gene="yecN"
                     /locus_tag="ACHKBL_003419"
     CDS             6018..6413
                     /gene="yecN"
                     /locus_tag="ACHKBL_003419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HFO5820245.1"
                     /translation="MVSALYAVLSALLLMKFSFDVVRLRMQYRVAYGDGGFSELQSAI
                     RIHGNAVEYIPIAIVLMLFMEMNGAETWMVHICGIVLLAGRLMHYYGFHHRLFRWRRS
                     GMSATWCALLLMVLANLWYMPWELVFSLR"
     gene            6454..7197
                     /gene="cmoA"
                     /locus_tag="ACHKBL_003420"
     CDS             6454..7197
                     /gene="cmoA"
                     /locus_tag="ACHKBL_003420"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HFO5820246.1"
                     /translation="MSHRDTLFSAPIARLGDWTFDERVAEVFPDMIQRSVPGYSNIIS
                     MIGMLAERFVQPGTQVYDLGCSLGAATLSVRRNIHHDNCKIIAIDNSPAMIERCRRHI
                     DAYKAPTPVDVIEGDIRDIAIENASMVVLNFTLQFLEPSERQALLDKIYQGLNPGGAL
                     VLSEKFSFEDAKVGELLFNMHHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARL
                     HKAGFEHSELWFQCFNFGSLVALKAEDAA"
     gene            7194..8165
                     /gene="cmoB"
                     /locus_tag="ACHKBL_003421"
     CDS             7194..8165
                     /gene="cmoB"
                     /locus_tag="ACHKBL_003421"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HFO5820247.1"
                     /translation="MIDFGNFYSLIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSN
                     AVEFLPEIKPYRLDLLHSVTAESEEPLSAGQIKRIETLMRNLMPWRKGPFSLYGVNID
                     TEWRSDWKWDRVLPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQ
                     FEAVRKLLGNDQRAHLLPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVN
                     EGELVLETLVIDGDENTVLVPGDRYAQMRNVYFIPSALALKNWLKKCGFVDIRIADVS
                     VTTTEEQRRTEWMVTESLADFLDPHDPGKTVEGYPAPKRAVLIARKP"
     gene            complement(8330..10759)
                     /gene="torZ"
                     /locus_tag="ACHKBL_003422"
     CDS             complement(8330..10759)
                     /gene="torZ"
                     /locus_tag="ACHKBL_003422"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine N-oxide reductase TorZ"
                     /protein_id="HFO5820248.1"
                     /translation="MTLTRREFIKHSGIAAGALVVTSAAPLPAWAEEKGGKILTAGRW
                     GAMNVEVKDGKIVSSTGALAKTIPNSLQSTAADQVHTTARIQHPMVRKSYLDNPLQPA
                     KGRGEDTYVQVSWEQALKLIHEQHDRIRKANGPSAIFAGSYGWRSSGVLHKAQTLLQR
                     YMNLAGGYSGHSGDYSTGAAQVIMPHVVGSVEVYEQQTSWPLILENSQVVVLWGMNPL
                     NTLKIAWSSTDEQGLEYFHQLKKSGKPVIAIDPICSETIEFFGDNATWIAPNMGTDVA
                     LMLGIAHTLMTQGKHDKVFLEKYTTGYPQFEEYLTGKSDNTPKSAAWAAEITGVPEAQ
                     IVKLAELMAANRTMLMAGWGIQRQQYGEQKHWMLVTLAAMLGQIGTPGGGFGFSYHYS
                     NGGNPTRVGGVLPEMSAAIAGQASEAADDGGMTAIPVARIVDALENPGGKYQHNGKEQ
                     TYPNIKMIWWAGGGNFTHHQDTNRLIKAWQKPEMIVVSECYWTAAAKHADIVLPITTS
                     FERNDLTMTGDYSNQHIVPMKQAVAPQFEARNDFDVFADLAELLKPGGKEIYTEGKDE
                     MAWLKFFYDAAQKGARAQRVTMPMFNAFWQQNKLIEMRRSEKNEQYVRYGDFRADPVK
                     NALGTPSGKIEIYSKTLEKFGYKDCPAHPTWLAPDEWKGSADEKQLQLLTAHPAHRLH
                     SQLNYAELRKKYAVADREPITIHTEDAARFGIANGDLVRVWNKRGQILTGAVVTDGIK
                     KGVVCVHEGAWPDLENGLCKNGSANVLTADIPSSQLANACAGNSALVYIEKYTGNAPK
                     LTAFDQPAVQA"
     gene            complement(10784..11884)
                     /gene="torY"
                     /locus_tag="ACHKBL_003423"
     CDS             complement(10784..11884)
                     /gene="torY"
                     /locus_tag="ACHKBL_003423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HFO5820249.1"
                     /translation="MRGKKRIGLLFLLIAVVVGGGGLLLAQKALHKTSDTAFCLSCHS
                     MSKPFEEYQGTVHFSNQKGIRAECADCHIPKSGMDYLFAKLKASKDIYHEFVSGKIDS
                     DDKFEAHRQEMAETVWKELKATGSATCRSCHSFDAMDIASQSESAQKMHNKAQKDGET
                     CIDCHKGIAHFPPEIKMDDNAAHELESQAATSVTNGAHIYPFKTSRIGELATVNPGTD
                     LTVVDASGKQPIVLLQGYQMQGSENTLYLAAGQRLALATLSEEGIKALTVNGEWQADE
                     YGNQWRQASLQGVLTDPALADRKPLWQYAEKLDDTYCAGCHAPIASDHYTVNAWPSIA
                     KGMGARTSMSENELDILTRYFQYNAKDITEKQ"
BASE COUNT         2734 a         3178 c         2930 g         3129 t
ORIGIN      
        1 acgtgaatgt taattatcct gctgaatatg aaattggtga tatcgtcttt acatgtatag
       61 gtgctacctt atttggtcaa atatcagctg catcaaattg ctggagtaat cacgtcggga
      121 tcattatcgg tcataacggt gaagacttcc tggttgcaga aagccgggtt cccctttcaa
      181 ccatcaccac gctatctcgt tttattaaac gttcgtctaa tcaacgctat gctataaagc
      241 gattagacgc tgggctgaca gaacaacaaa aacaacgaat tgttgaacag gttccctccc
      301 ggctacgcaa actttaccac accggtttta aatacgaatc ttcgcgccag ttctgttcaa
      361 aatttgtttt tgatatttat aaagaagcgc tatgtattcc ggtgggtgaa atagagacgt
      421 ttggagaatt gttaaatagc aatccgaatg caaaactcac tttctggaaa ttctggttct
      481 taggttctat tccgtgggag cgtaaaaccg tcacgccagc cagtttgtgg catcatccgg
      541 gtttggtgtt gattcatgcg gagggagtgg aaacgcctca gccggaactg accgaggcgg
      601 tataacttaa cgcagtcgcc ctctcgccag gttcagccgc gattcgctca tctgcatcgc
      661 attctgactg acgtgacaat gggtaatcgc gatagctaat gcatcggcag catccgcctg
      721 tggattagcg ggcagtttca gcaaggtgcg gaccatatgc tgcacctggc ttttttcggc
      781 actaccaata cctaccaccg tttgctttac ctgacgtgcc gcatattcaa ataccggcaa
      841 ttcctgattc accgctgcca caatcgccac gccgcgcgcc tgacccagtt tcagggctga
      901 gtcagcgttc ttcgccataa agacttgttc aatggcgaaa taatcaggct ggaactgggt
      961 gatgatttcc gtcacgcccg catagatgag cttcagacga gacggtaaat catccacttt
     1021 ggtgcgtatg catccgctac ccaggtagga cagttgcctg cctacctggc ggatgacgcc
     1081 atagccggtc acgcgcgaac ccgggtcaat gccgagaata atagccatca cgcgtctccg
     1141 ttttgctgtt tagcaggcct catcagagag tcgctgcaac ctcatcagag atttcaccgt
     1201 tatggtaaac ttcctgcacg tcgtcgcaat cttccagcat atcgatcaga cgcatcagtt
     1261 tcggtgcggt ttctgcatcc atatcagctt tggtggacgg gatcatggaa acttccgcgc
     1321 tgtctgcttt cagacctgcc gcttccagag cgtcgcgtac tttgcccatt tcttcccatg
     1381 cagtgtagac atcaatcgcg ccgtcatcat aggtcacaac gtcttcagca ccggcttcca
     1441 gggctgcttc catgatggtg tcttcatcgc ctttctcgaa ggagatcacg ccttttttgc
     1501 tgaacagata agctacggaa ccatcagtac cgaggttacc gccacattta ctgaatgcat
     1561 ggcgcacttc agcaacggta cggttgcggt tgtcagacag acattcaatc atgattgccg
     1621 tgccgccagg accgtaacct tcgtagatga tggtttccat gtttgcatca tcatcaccgc
     1681 ccacacctcg tgcaattgcg cggttcagag tgtcacgggt catgttgtta gacagtgctt
     1741 tatcaattgc tgcacgcaga cgcgggttag cgtccggatc accaccgccc agcttagccg
     1801 cggttaccag ctcacgaatg attttagtga agattttacc gcgcttagca tcctgcgcag
     1861 ctttacgatg tctggtgttg gcccatttac tatgacctgc cataaaaata tctccagata
     1921 gccctgcctg ttcaggcagc gttaattaca aactgttcaa tcgcctgccg gttgctccag
     1981 gacttagtga gcgccgcagc agcaggcgcg tcaagccact tgtaagccag atgttcagtg
     2041 aaaacgatct ggcgctcgtg cgggagcgca agacagaacc atgattccgt attacgcgtc
     2101 acgcccggcg catagcgatg acgtaaatgt gaaaaaattt caaactctac cgtgcgctga
     2161 cagtcaatta aggtcagttg ttcagcgaca acatcaatgg tgacctcttc ctttacttcg
     2221 cgcatggcag cttgcggcgc ggtttcaccc tcttccacgc tgccggttac cgactgccag
     2281 aaatcgggat cgtcacgccg ctgcaacatc agcacccgtt tcgtatcttg tgcatagatg
     2341 accactaaga tcgaaacggg acgcttatac actttatcct tcacgctgcc tcttcgttga
     2401 ctgccttcgc tcatcccatt cacatagtta tctatgctca tgggagttca ctcagttgcc
     2461 gcctcgatgc aacgcgaatg atttcgtgta tttgagtcat atcagttatt ctcgtccttc
     2521 ttcacaacct gaatgctcag ctcagccagt gcagtcgggt tagcaaagct cggtgcttca
     2581 gtcatcagac acgctgccgc cgtggttttc gggaaggcga taacgtcacg gatattgtcg
     2641 gtgccggtca gcagcatggt cagacggtca agaccgaatg ccagacctgc gtgcggcgga
     2701 gtaccgtatt tcagggcgtc gagcaggaag ccgaatttct cgcgctgttc ctcttcgttg
     2761 atacccagaa taccaaacac cgtctgctgc atatcaccat tatggatacg cacagaacca
     2821 ccgcccactt cgtaaccatt gatgaccata tcgtaagcgt tcgccaccgc attttctggt
     2881 gcagctttca gttctgcagc agtcatgtct ttcggtgagg tgaacggatg gtgcattgcc
     2941 gtcaggccgc cttcaccgtc gtcttcaaac atcgggaagt cgataaccca cagcggtgcc
     3001 catttgcttt cgtcggtcag accaaggtct ttacccactt tcaggcgcag tgcgcccatc
     3061 gcgtcggcaa caattttctt gttgtcggca ccgaagaaaa tcatatcgcc atcttgcgcg
     3121 gcagtacgat ccaggatggc ttcgatgatt tctgcattaa ggaacttcgc taccgggctg
     3181 ttgatacctt ccagaccttt cgcgcgttcg ttaactttga tgtaagccag acctttcgcg
     3241 ccgtagattt taacgaagtt accgtattcg tcgatctgct tacgggtcaa cgatgcgccg
     3301 cccggaacac gcagagcggc aacacggcct ttcggatcgt tcgccggacc tgcaaatact
     3361 gcaaactcaa cagatttcag cagatcggca acgtcggtca gttccatcgg gttacgcaga
     3421 tccggtttat cagaaccata acggcgttct gcttctgcaa aggtcattac cgggaaatcg
     3481 cccagatcca cgcccttcac ttccagccac agatgacgca ccagcgcttc catcacttca
     3541 cgcacttgcg gcgcggtcat gaaagaagtt tccacatcga tctgagtaaa ttcaggctga
     3601 cggtcagcac gcaggtcttc gtcacggaag catttaacga tctgatagta gcggtcaaag
     3661 ccggacatca tcagtagctg tttgaacaac tgcggggatt gcggcagcgc gtagaattta
     3721 cctttgtgca cacgagaagg caccaggtag tcacgcgcgc cttccggcgt ggctttggtc
     3781 agcatcggag tttcgatgtc gaggaagccg tggtcatcca taaaacggcg caccaggctg
     3841 gtgattttag cgcgggtttt caggcgctga gccatttccg ggcgacgcag gtcgaggtag
     3901 cggtatttca gacgcgcttc ttcggtgttg acgtggttag agtcaagcgg cagaacatct
     3961 gcacgattga tgatagtcag cgaggacgcc agtacttcga tttcgccagt cgccatatcg
     4021 cggttaatat ttttttcgtc acgcgcacgt acggtgcccg tgacctgaat gcagaactca
     4081 ttacgcagtt cagaggccag ctttaacgcg tccgcacgat ccggatcgaa aaatacctgc
     4141 acgatacctt cgcggtcgcg catatcgatg aagatcaggc taccaagatc acgacgacgg
     4201 ttgacccaac cacacagagt cacctgctgc cccacgtggg acaaacggag ctgtccacaa
     4261 tattctgtac gcatgagata tcccttaact tagctgccgg cggatgcccc ctgctgcgca
     4321 ggtgaccaag tcgcagcgtt agctgtatgt cacaactgaa tgaaaaaagg cggctattat
     4381 actggaaatt ctgccgcacc gtaagagcct ggcccgcgct ggaacgcctc gttaccactt
     4441 tatatcgggc ctgaaatcag actctacgcc agtttgctat aaaggtgttg cccgaactca
     4501 taaaaattaa caaaatttgt cgttccgcca tcggctaatc gcattaaggt gagaggcacg
     4561 attttgtttt gtcaggagtc atcatgcttg aacttaatgc taaaaccacc gcgctggtgg
     4621 tgattgattt acaagaaggc atcttacctt ttgccggagg tccacatact gccgatgagg
     4681 tggttaatcg cgccgggaag ctggcggcga aatttcgcgc cagcggtcag cccgtgtttc
     4741 tggtgcgcgt tggctggtct gccgattacg ccgaagcatt aaaacagccg gttgatgccc
     4801 cctcccccgc aaaagtgttg cccgaaaact ggtggcaaca tcctgctgca ttaggtgcaa
     4861 ccgacagcga tatcgaaatc atcaaacgtc aatggggtgc gttttacggt acggatctgg
     4921 agttgcaatt acgccgccgg ggtatcgata caatagtgtt atgtgggatc tcgaccaata
     4981 tcggtgttga atccaccgcc cgcaatgcct gggaactcgg ttttaatctg gtgattgccg
     5041 aagatgcctg tagcgccgcc agcgccgagc agcacaataa cagcattaat catatctacc
     5101 cgcgcatcgc ccgtgtgcgc agtgtggaag agatcctcca cgcgttatga tttacatcgg
     5161 tctgccgcaa tggtcgcatc ctaaatgggt gcggttgggg atcaccaacc ttgaagagta
     5221 tgcccgccac tttaactgcg tggagggtaa caccacgctt tatgccctgc cgaaacccga
     5281 ggttgtcctg cgctggcgtg agcagaccac agatgacttc cgcttctgtt ttaagtttcc
     5341 ggcgaccatt tcgcatcagg cagcattacg gcattgcgat gatttagtga ctgaattttt
     5401 gacccgcatg tcaccgttgg ctccgcgcat tggacaatac tggctgcaac tgcctgccac
     5461 attcggccca cgagagctgc ctgcgctttg gcattttctc gattctcttc ccggtgaatt
     5521 taattatggg gtggaagtcc gccatccaca gtttttcgcc aaaggggaag aggaacaaac
     5581 gcttaatcgc ggtttacatc agcgcggcgt taatcgggtg attttagaca gccgcccggt
     5641 tcatgcagca cgtccacaca gtgaagctat tcgcgacgct caacgaaaaa aacctaaagt
     5701 tccggtacat gctgtactga cggcgaaaaa tccactgatc cgttttatcg gtagtgatga
     5761 tatgacgcaa aaccgggagt tatttcaggt ctggttacaa aaattagcac agtggcatca
     5821 gaccactacg ccttatcttt ttttacatac gccagatatt gcccaggccc cggaactggt
     5881 acataccctg tgggaagact tacgtaaaac gcttccagag atcggagcag ttccggctat
     5941 tccacagcaa tcttctcttt tctgaatttg ccacctatca tagacaggtg ccatcggcca
     6001 ttttaaaggg agtttgtatg gtaagcgcgc tgtatgccgt tttaagtgcg ttgttattaa
     6061 tgaagttctc ttttgatgtc gttcgcctgc gaatgcagta ccgcgttgcc tatggcgacg
     6121 gcggttttag cgaactgcaa agcgctattc gcattcatgg taacgcggtg gaatatattc
     6181 ctatcgcgat tgtgttgatg ctgtttatgg aaatgaatgg cgcagaaacc tggatggtgc
     6241 atatttgcgg catcgttttg cttgctggtc gtctgatgca ttattacggt tttcatcacc
     6301 gtctgttccg ctggcgacgt tctggcatga gcgcgacctg gtgtgcgctg ttgctgatgg
     6361 tgctggcgaa tctttggtat atgccctggg agttggtttt ctccctgcgt tagcgcacaa
     6421 tacgccactt tctttttccc ggatttttac gttatgtctc accgcgacac gctattttct
     6481 gcccctatcg ccagactggg cgactggacc tttgatgaac gggtagctga agtcttcccg
     6541 gatatgatcc agcgttccgt tcccggctat tccaatatta tttccatgat tggtatgtta
     6601 gccgagcgct tcgttcaacc tggtacgcag gtttacgatc tgggttgttc tctgggcgcg
     6661 gcgacgctct cggtgcgtcg caacattcat catgataatt gcaaaattat tgccatcgat
     6721 aactcccccg cgatgattga acgctgccgt cgtcatattg acgcctataa agcccctacg
     6781 ccagtagacg tcattgaagg tgatattcgc gatatcgcca ttgaaaacgc atcgatggtg
     6841 gtgctgaatt ttaccctgca attcctggaa ccttccgagc gccaggcgtt actggataaa
     6901 atttatcaag ggctgaaccc gggcggcgcg ctggtgcttt cggaaaaatt cagtttcgaa
     6961 gatgccaaag ttggtgaact gttgttcaac atgcaccacg actttaaacg tgccaacggt
     7021 tacagcgaac tggagatcag ccagaagcgc agcatgctgg aaaacgtgat gctgaccgat
     7081 tccgtggaaa cccataaagc acgcctgcat aaagctggtt ttgaacatag tgagctgtgg
     7141 ttccagtgct ttaactttgg ttcactggtg gcattaaaag cagaggacgc tgcatgatcg
     7201 actttggtaa cttttattct ctgattgcca aaaatcatct ttcacactgg ctggaaacgc
     7261 tgcccgcgca gattgctaac tggcagcgcg agcagcagca cgggctgttt aagcagtggt
     7321 ccaacgcggt ggaatttctg cctgaaatta aaccgtatcg tctggattta ttgcatagcg
     7381 taaccgccga aagcgaagag ccactgagcg ccgggcaaat taaacgcatt gaaacgctga
     7441 tgcgcaacct gatgccgtgg cgcaaagggc cgttctcact gtatggcgtc aacatcgata
     7501 ccgaatggcg ttccgactgg aaatgggatc gcgttctgcc ccatctttct gatttaaccg
     7561 ggcgcaccat tcttgatgtc ggctgtggca gcggttatca catgtggcgc atgattggcg
     7621 caggcgcgca cctcgcggtg ggaatcgacc ctacgcaact gtttctgtgc cagtttgaag
     7681 cggtgcgtaa actactgggt aacgatcagc gcgcacattt gttaccgtta ggtattgaac
     7741 agcttccggc attgaaagcc tttgataccg tcttttcgat gggcgtgctt tatcatcgtc
     7801 gttcaccgct ggagcatctc tggcagttaa aagaccaact ggtgaatgaa ggtgaactgg
     7861 tgctggaaac gctggttatt gatggtgatg agaacacggt gctggtgccg ggcgatcgtt
     7921 acgcgcaaat gcgtaatgtc tatttcatcc cttccgcgct ggcgctgaaa aactggctga
     7981 agaagtgtgg ttttgtagat atccgcattg cggatgtgag cgttaccacc acagaagagc
     8041 agcgacgcac cgaatggatg gtcaccgagt ctctggccga ttttctcgac ccgcatgatc
     8101 cgggtaaaac ggtggaaggt tatcctgcgc ctaaacgcgc ggtgctgatt gcgcgcaagc
     8161 cgtaaaggtc tggtattact gccggatgcg gcgtgaacgc cttatccggc ctacaaaacc
     8221 ttgctaattc aatatattgc agggactatg taggccttat aagcatagcg catcaggcag
     8281 ttttacgttt gcataacctc agcgcccgtt tccgggcgct tttcacgtct tatgcctgaa
     8341 cagctggctg atcaaacgcc gttaacttcg gtgcattgcc cgtatatttt tcgatataca
     8401 ccagcgcaga gttaccggca caggcatttg ccagctgaga gctggggata tccgccgtta
     8461 acacgttcgc actgccgttt ttacacaagc cattttccag atctggccat gcaccttcat
     8521 gcacgcatac cacgcctttt ttgatcccgt cagtcaccac cgcgcctgtc agaatctgac
     8581 cacgtttgtt ccagacacgc accagatcgc cattcgcaat accaaagcga gcagcatctt
     8641 cggtgtgaat agtgatcggt tcacgatcgg caaccgcata ttttttacgc agttccgcat
     8701 agttaagctg actgtgtaaa cggtgtgccg gatgtgcggt cagaagctgc aactgcttct
     8761 cgtcggcgga acctttccac tcatcaggcg caagccaggt tgggtgtgcc gggcaatcct
     8821 tatagccaaa tttttccagc gttttggagt aaatctcaat tttgccgctt ggcgtaccca
     8881 gcgcattttt caccggatcg gcgcggaaat caccataacg aacgtattgt tcgttcttct
     8941 cactgcggcg catttcgatc agtttatttt gctgccagaa agcattaaac atcggcatag
     9001 tcacgcgttg cgcacgggca cctttctgag cggcatcata gaaaaatttc agccacgcca
     9061 tttcatcttt accttcggta tagatctctt ttccgccagg tttgagtaat tcagcaagat
     9121 cggcaaacac gtcaaaatcg ttgcgcgctt caaattgcgg agcgacagcc tgcttcatcg
     9181 gcacaatatg ctggttgctg taatcaccgg tcatcgtcag gtcattgcgc tcaaacgatg
     9241 tggtgatcgg taatacgata tctgcatgtt tagcagctgc ggtccagtag cattcagaaa
     9301 cgacgatcat ctccggtttc tgccatgctt taatcagacg attagtatcc tggtgatggg
     9361 taaagttacc gccacccgcc caccagatca ttttgatatt cggatacgtt tgttctttac
     9421 cgttatgctg atatttgccg ccaggatttt ccagcgcatc gacgatacgg gcaacaggaa
     9481 tcgccgtcat tccgccatca tcagcggcct cgctggcctg tccggcaatc gccgcagaca
     9541 tttccggcag cacgccgcca acacgcgtcg ggttgccacc gttggagtaa tgataagaga
     9601 atccaaaacc gccgcccggt gtaccaattt gccctaacat cgccgccagc gtcaccagca
     9661 tccagtgttt ctgttcaccg tattgctggc gttggatccc ccagcctgcc atcagcatcg
     9721 tgcgattcgc cgccatcagt tctgcaagtt tgacaatctg cgcttccggt acgccagtaa
     9781 tttccgccgc ccaggccgcg ctcttcggcg tattatcgct cttacctgtc agatactctt
     9841 caaactgcgg atacccggta gtgtattttt caaggaacac tttatcgtgt ttgccttgtg
     9901 tcatcagggt atgcgcaatc cctaacatca gtgccacgtc ggtgcccata ttcggtgcga
     9961 tccaggtggc attatcgcca aagaattcga tggtttcgga gcagatagga tcaatggcaa
    10021 tcactggttt gccagatttt ttcagctgat ggaagtattc cagcccttgc tcatcggtac
    10081 tgctccaggc aattttgagc gtgttcagcg ggttcatccc ccacagcacc acaacctggc
    10141 tgttttccag aatcagcggc caggaggtct gctgttcata cacctctaca gaaccgacca
    10201 catgcggcat gatcacctgt gccgctccgg ttgaataatc gccgctatgc ccggaataac
    10261 cgcccgccag gttcatataa cgttgcagta aggtttgcgc tttatgcaac acgccagaag
    10321 aacgccagcc gtaagatccg gcaaaaatgg ccgatggtcc gttagcttta cgaatacgat
    10381 cgtgttgctc atgaatcagt tttaatgctt gttcccagct cacctgtaca taggtatctt
    10441 cgccacgacc tttcgccggt tgcagtggat tatcgagata gctttttctc accatcggat
    10501 gctgaatacg cgccgtggtg tgtacctgat ccgccgccgt agactgtaag gagttcggta
    10561 tggttttcgc cagcgcgcct gtcgaagaaa caatcttgcc gtccttcact tctacgttca
    10621 tcgctcccca acggcccgcg gtgagaattt tgccgccctt ctcttctgcc catgcaggta
    10681 acggtgcagc cgatgtcacc accagcgccc ctgccgcaat accgctatgt ttaataaatt
    10741 cacgtcttgt taatgtcata acttcctccc tgatcaacga ggatcactgt ttctcggtaa
    10801 tatctttggc gttgtactgg aagtaccgcg tcaaaatgtc cagttcgttt tcgctcatgc
    10861 tggttcgcgc acccattcct ttggcaatgg acggccatgc attgacggtg taatggtcgg
    10921 aggcaatagg ggcatggcaa ccagcgcaat aggtatcgtc aagtttttca gcgtattgcc
    10981 atagaggttt acggtccgct aatgcgggat cggtaagcac accctgtaaa gacgcctgac
    11041 gccattgatt gccgtattcg tcagcctgcc attccccgtt taccgtcagc gccttgatac
    11101 cttcttcact taatgtggct agcgccagcc gttgacctgc cgccaggtag agcgtgtttt
    11161 cactgccctg catttgataa ccctgcaaca gaacgatcgg ctgtttgcca ctggcatcaa
    11221 cgacggtgag atcggtacca ggattcacgg tagccagttc gcctatgcga gaagttttga
    11281 aaggataaat atgtgcgcca ttagtaactg aagtagcggc ctgactttcc agctcatgcg
    11341 ccgcgttgtc atccattttt atttctggcg gaaaatgggc aatgccttta tggcaatcga
    11401 tacaggtttc gccgtccttt tgcgctttat tatgcatttt ctgcgcagat tcactttgcg
    11461 aggcaatatc catggcatca aaagaatggc aactacggca ggttgcagag ccagtggctt
    11521 ttaattcttt ccatactgtt tcggccattt cctggcgatg agcttcgaac ttatcatcac
    11581 tgtctatttt gccgctaaca aattcatgat aaatatcttt agatgctttt aatttagcaa
    11641 ataaataatc catccctgac tttggaatat ggcaatcggc acattccgca cgtatccctt
    11701 tctggtttga aaagtggaca gttccctgat attcctcaaa aggtttactc atcgagtggc
    11761 aggaaaggca aaatgcggta tccgacgttt tatgtaaggc tttttgcgcc agtaataacc
    11821 cgccgccacc aaccacaacc gctatcagca aaaacaataa cccaatgcgt tttttccctc
    11881 gcatagattc ctctaattaa attccaggac accgatacca ttgtataaat aaatacacaa
    11941 cgaattgact acttgcttaa ttcggcacaa c
//
LOCUS       DAZFLD010000143        11839 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.144, whole genome shotgun sequence.
ACCESSION   DAZFLD010000143 DAZFLD010000000
VERSION     DAZFLD010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11839)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11839)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11839
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.144"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            106..1383
                     /gene="waaA"
                     /locus_tag="ACHKBL_003424"
     CDS             106..1383
                     /gene="waaA"
                     /locus_tag="ACHKBL_003424"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HFO5820250.1"
                     /translation="MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRH
                     PLKPGGIMLHSVSVGETLAAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQ
                     HVYLPYDLPDALNRFLNKVDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSA
                     AGYAKLGKFVRRLLRRITLIAAQNEEDGARFVALGAKNNQVTVTGSLKFDISVTPQLA
                     AKAVTLRRQWAPHRPVWIATSTHEGEESVVIAAHQALLQQFPNLLLILVPRHPERFPD
                     AINLVRQAGLSYITRSSGEVPSTSTQVVVGDTMGELMLLYGIADLAFVGGSLVERGGH
                     NPLEAAAHAIPVLMGPHTFNFKDICARLEQASGLITVTDATSLAKEVSSLLTDADYRS
                     FYGRHAVEVLYQNQGALQRLLQLLEPYLPPKTH"
     gene            1391..1870
                     /gene="coaD"
                     /locus_tag="ACHKBL_003425"
     CDS             1391..1870
                     /gene="coaD"
                     /locus_tag="ACHKBL_003425"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HFO5820251.1"
                     /translation="MQKRAIYPGTFDPITNGHIDIVTRATQMFDHVILAIAASPSKKP
                     MFTLEERVALAQQATAHLGNVEVVGFSDLMANFARNQHATVLIRGLRAVADFEYEMQL
                     AHMNRHLMPELESVFLMPSKEWSFISSSLVKEVARHQGDVTHFLPENVHQALMAKLA"
     gene            complement(1909..2718)
                     /gene="mutM"
                     /locus_tag="ACHKBL_003426"
     CDS             complement(1909..2718)
                     /gene="mutM"
                     /locus_tag="ACHKBL_003426"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HFO5820252.1"
                     /translation="MPELPEVETSRRGIEPHLVGATILHAVVRNGRLRWPVSEEIYRL
                     SDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKV
                     LRYTDPRRFGAWLWTKELEGHNVLAHLGPEPLSDDFNGEYLHQKCEKKKTAIKPWLMD
                     NKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLK
                     DFLQSDGKPGYFAQELQVYGRKGESCRVCGTPIVATKHAQRATFYCRQCQK"
     gene            complement(2816..2983)
                     /gene="rpmG"
                     /locus_tag="ACHKBL_003427"
     CDS             complement(2816..2983)
                     /gene="rpmG"
                     /locus_tag="ACHKBL_003427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFO5820253.1"
                     /translation="MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVR
                     QHVIYKEAKIK"
     gene            complement(3004..3240)
                     /gene="rpmB"
                     /locus_tag="ACHKBL_003428"
     CDS             complement(3004..3240)
                     /gene="rpmB"
                     /locus_tag="ACHKBL_003428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HFO5820254.1"
                     /translation="MSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEK
                     RFVTLRVSAKGMRVIDKKGIDTVLAELRARGEKY"
     gene            complement(3457..4125)
                     /gene="radC"
                     /locus_tag="ACHKBL_003429"
     CDS             complement(3457..4125)
                     /gene="radC"
                     /locus_tag="ACHKBL_003429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418095.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFO5820255.1"
                     /translation="MKNNSQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTL
                     AKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQLKGIAELARRYYNVRMREESPLL
                     SPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
                     KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAE
                     RGWI"
     gene            4297..5517
                     /gene="coaBC"
                     /locus_tag="ACHKBL_003430"
     CDS             4297..5517
                     /gene="coaBC"
                     /locus_tag="ACHKBL_003430"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015941 - pantothenate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HFO5820256.1"
                     /translation="MSLAGKKIVLGVSGGIAAYKTPELVRRLRERGADVRVAMTEAAK
                     AFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARVAAGM
                     ANDLVSTICLATPAPVAVLPAMNQQMYRAAATQHNLEVLASRGLLIWGPDSGSQACGD
                     IGPGRMLDPLTIVDMAVAHFSPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGF
                     AIAAAAARRGANVTLVSGPVSLPTPPFVKRVDVMTALEMEAAVNASVQQQNIFIGCAA
                     VADYRAATVAPEKIKKQATQGDELTIKMVKNPDIVAGVAALKDHRPYVVGFAAETNNV
                     EEYARQKRIRKNLDLICANDVSQPTQGFNSDNNALHLFWQDGDKVLPLERKELLGQLL
                     LDEIVTRYDEKNRR"
     gene            5495..5953
                     /gene="dut"
                     /locus_tag="ACHKBL_003431"
     CDS             5495..5953
                     /gene="dut"
                     /locus_tag="ACHKBL_003431"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HFO5820257.1"
                     /translation="MMKKIDVKILDPRVGKEFPLPTYATSGSAGLDLRACLDDAVELA
                     PGDTTLVPTGLAIHIADPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVW
                     NRGQDSFTIQPGERIAQMIFVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ"
     gene            6060..6656
                     /gene="slmA"
                     /locus_tag="ACHKBL_003432"
     CDS             6060..6656
                     /gene="slmA"
                     /locus_tag="ACHKBL_003432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0010974 - negative regulation of division
                     septum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HFO5820258.1"
                     /translation="MAEKQTAKRNRREEILQSLALMLESSDGSQRITTAKLAASVGVS
                     EAALYRHFPSKTRMFDSLIEFIEDSLITRINLILKDEKDTTARLRLIVLLLLGFGERN
                     PGLTRILTGHALMFEQDRLQGRINQLFERIEAQLRQVLREKRMREGEGYTTDETLLAS
                     QLLAFCEGMLSRFVRSEFKYRPTDDFDARWPLIAAQLQ"
     gene            complement(6693..7334)
                     /gene="pyrE"
                     /locus_tag="ACHKBL_003433"
     CDS             complement(6693..7334)
                     /gene="pyrE"
                     /locus_tag="ACHKBL_003433"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HFO5820259.1"
                     /translation="MKPYQRQFIEFALSKQVLKFGEFTLKSGRKSPYFFNAGLFNTGR
                     DLALLGRFYAEALVDSGIEFDLLFGPAYKGIPIATTTAVALAEHHDLDLPYCFNRKEA
                     KDHGEGGNLVGSALQGRVMLVDDVITAGTAIRESMEIIQANGATLAGVLISLDRQERG
                     RGEISAIQEVERDYNCKVISIITLKDLIAYLEEKPEMAEHLAAVKAYREEFGV"
     gene            complement(7400..8116)
                     /gene="rph"
                     /locus_tag="ACHKBL_003434"
     CDS             complement(7400..8116)
                     /gene="rph"
                     /locus_tag="ACHKBL_003434"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006803115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HFO5820260.1"
                     /translation="MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIE
                     EGVPRFLKGQGQGWITAEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAV
                     DLKALGEFTITLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKTNPMKGMVA
                     AVSVGIVNGEAVCDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLL
                     ALARGGIESIVATQKAALAN"
     gene            8243..9106
                     /locus_tag="ACHKBL_003435"
     CDS             8243..9106
                     /locus_tag="ACHKBL_003435"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HFO5820261.1"
                     /translation="MIRSMTAYARREIKGEWGSATWEMRSVNQRYLETYFRLPEQFRS
                     LEPVVRERIRSRLTRGKVECTLRYEPDVSAQGELILNEKLAKQLVTAANWVKMQSDEG
                     EINPVDILRWPGVMAAQEQDLDAIAAEILAALDGTLDDFIVARETEGQALKALIEQRL
                     EGVTAEVVKVRAHMPEILQWQRERLVAKLEDAQVQLENNRLEQELVLLAQRIDVAEEL
                     DRLEAHVKETYNILKKKEAVGRRLDFMMQEFNRESNTLASKSINAEVTNSAIELKVLI
                     EQMREQIQNVE"
     gene            9297..10559
                     /locus_tag="ACHKBL_003436"
     CDS             9297..10559
                     /locus_tag="ACHKBL_003436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001303105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO5820262.1"
                     /translation="MPKLTDIQIRAWIKADERFDGKADGNGLYLCYPKNYTMPFWRFR
                     YKFAGKQRAMVIGSYAELSLSRARETAKELSARVALGYDVAAEKQERKAEALAKMEAE
                     KNAIRVSELAAEYFERQILPRWKHPDILRRRIDKDINPRIGHMKAEDVKPRHIDEMLK
                     AIVDRGAPTIATDVLRWTRRIFDYGIKRHVLEINPCSAFEVSDAGGKESSRDRWLTRE
                     ELIRFFQAMRTAKGFSRQNELTFKLLLALCVRKMELCAARWEEFDLDNAVWHLPEERS
                     KNGDPIDIPLVPPVIEWLKELRVLSCNSAWVLPARKMQHRMIPHIQESTLPVALAKVR
                     AEMPEVPDFTIHDFRRTARTHLAALGVDPVVAERCLNHRIKGVEGIYNRYQYFDERTS
                     ALAKWTNLLVSLERGERYNVTPIRRAKA"
     gene            10556..11206
                     /locus_tag="ACHKBL_003437"
     CDS             10556..11206
                     /locus_tag="ACHKBL_003437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5820263.1"
                     /translation="MINILKKSPIERLCASVSITPHEMALALAGLNPSMRIGDVPQDK
                     FEQVESARTMIARAIWLHSGKKAGKDEPYRAGDIFLASFPFIEAGTPEAIITAVTDAI
                     DDLRGTKNWEEKALNLGGRRLVSHIKETSRSGRGQYRKLDEEQGNMKMMGLLVLLLVK
                     KSGTTAYIQDGEPNRSAIYRDVEALMKEKGISPKGIAKSTFMQKISAALLAVSQAD"
     gene            11593..11808
                     /locus_tag="ACHKBL_003438"
     CDS             11593..11808
                     /locus_tag="ACHKBL_003438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000126307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO5820264.1"
                     /translation="MTANHQEAIPTTGYIRRCNLAKLLDTSVSTIDRWVRIGKLPKPT
                     KLGEKNTVFNAVEINHWLNERRNNTKQ"
BASE COUNT         2950 a         3123 c         2998 g         2768 t
ORIGIN      
        1 aattggcggg taaattgcta tttaagtcaa aaacgggaaa agtaatggta aagccacagc
       61 taaatacata gaatccccag cacatccata agtcagctat ttactatgct cgaattgctt
      121 tacaccgccc ttctctacct tattcagccg ctgatctgga tacggctctg ggtgcgcgga
      181 cgtaaggctc cggcctatcg aaaacgctgg ggtgaacgtt acggttttta ccgccatccg
      241 ctaaaaccag gcggcatcat gctgcactcc gtctccgtcg gtgaaactct ggcggcgatc
      301 ccgttggtgc gcgcgctgcg ccatcgttat cctgatttac cgattaccgt tacaaccatg
      361 acgccaaccg gttcggagcg cgtacaatcg gctttcggga aggatgttca gcacgtttat
      421 ctgccatatg acctgcccga tgcacttaat cgtttcctga ataaagtcga ccctaaacta
      481 gtgttgatta tggaaaccga actatggcct aacctgattg cggcgctaca taaacgtaaa
      541 attccgctgg tgatcgctaa cgcgcgactc tctgcccgct cggccgcagg ttatgccaaa
      601 ctgggtaaat tcgtccgtcg cttgctgcgt cgtattacgc tgattgctgc gcaaaatgaa
      661 gaagatggtg cacgttttgt ggcgctgggc gcaaaaaata accaggtaac cgttaccggt
      721 agcctgaaat tcgatatttc tgtaacgccg cagttggctg ctaaagccgt gacgctgcgc
      781 cgccagtggg caccacaccg cccggtatgg attgccacca gcactcacga aggcgaagag
      841 agtgtggtga tcgccgcaca tcaggcattg ttacagcaat tcccgaattt attgctcatc
      901 ctggtccccc gtcatccgga acgcttcccg gatgcgatta accttgtccg ccaggctgga
      961 ctaagctata tcacacgctc ttcaggggaa gtcccctcca ccagcacgca ggttgtggtt
     1021 ggcgatacga tgggcgagtt gatgttactg tatggcattg ccgatctcgc ctttgttggc
     1081 ggttcactgg ttgaacgtgg tgggcataat ccgctggaag ctgccgcaca cgctattccg
     1141 gtattgatgg ggcctcatac ttttaacttt aaagacattt gcgcgcggct ggagcaggca
     1201 agcgggctga ttaccgttac cgatgccacg tcgcttgcaa aagaggtttc ctctttactc
     1261 accgacgccg attaccgtag tttctatggc cgtcatgccg ttgaagtact gtatcaaaac
     1321 cagggcgcgc tacagcgtct gcttcaactg ctggaacctt acctgccacc gaaaacgcat
     1381 tgaggttgtt atgcaaaaac gggcgattta tccgggtact ttcgatccca ttaccaatgg
     1441 tcatatcgat atcgtgacgc gcgccacgca aatgttcgat cacgttattc tggcgatcgc
     1501 cgccagtccc agtaaaaaac cgatgtttac tctggaagag cgtgtggcgc tggcacagca
     1561 ggcaaccgcg catctgggga acgtggaagt ggtcgggttt agtgatttga tggcgaactt
     1621 cgcccgtaat caacacgcta cggtgctgat tcgtggcctg cgtgcggtgg cggattttga
     1681 atatgaaatg cagctggcgc atatgaatcg ccacttaatg ccggaactgg aaagtgtgtt
     1741 tctgatgccg tcgaaagagt ggtcgtttat ctcttcatcg ttggtgaaag aggtggcgcg
     1801 ccatcagggc gatgtcaccc atttcctgcc ggagaatgtc catcaggcgc tgatggcgaa
     1861 gttagcgtag agtttatgcc ggatggtatg ccatccggcg cgcatcaatt acttctggca
     1921 ctgccgacaa taaaacgttg cccgctgcgc atgtttagtc gccacaatcg gcgtaccaca
     1981 cacccgacac gactcacctt ttcgcccgta aacctgcaat tcctgagcga aatagcccgg
     2041 tttaccatca ctttgcagaa aatctttcag cgttgtaccg ccctgctcaa tcgaacgcag
     2101 caacaccgct ttaatcaccc gagctaacaa ttcacactct gccagcgaca gtgatgacgc
     2161 cagccgatcc ggatggatcc ccgccgcaaa cagagattcg ctggcataga tattccctac
     2221 gcctaccacc agcttgttat ccatcagcca tggtttaatc gccgtttttt tcttctcgca
     2281 cttctgatgc aggtactcac cgttgaaatc gtcgctaagc ggctccggtc caagatgggc
     2341 cagcacgttg tgcccttcca gctctttggt ccacagccag gcaccaaagc ggcgcggatc
     2401 ggtgtagcgc agcactttac cgttgctcat caccaaatca acatggtcat gcttttcagg
     2461 gggaagttct tctggaagga tgcgcaggct gccagacatc cccaaatgaa tgataatcca
     2521 gccctcaggc agctccagca gcagatattt agcccgccgc tgcacgctaa gcactggttg
     2581 gtcgcttaaa cggtagatct cttctgaaac cggccagcgc aagcgtccgt tgcgcaccac
     2641 cgcatgaaga atggttgcac caacgagatg cggttctatg ccgcgacggc tggtttcaac
     2701 ttcgggtaat tcaggcatag catctccagg aatgaacaga tgcagtcaat atgggggcag
     2761 gcagataaca aaaaaccccg ccggggcgag gtttttcgtt acatcaaagc aaaaattatt
     2821 tgattttcgc ttctttgtag atcacgtgct ggcgaacaac tggatcgaat tttttcagtt
     2881 ccagtttttc cggcttagta cgtttgttct tcgtagtggt atagaagtga ccagtaccag
     2941 cagaagaaac cagcttgatt ttctcacgaa tacctttagc catgatttat ttcctctaag
     3001 tacttagtac ttttcgccac gggcacgcag ttcagccaga actgtatcga tgcctttttt
     3061 atcgattaca cgcatacctt tagcagatac gcgcagggtg acaaaacgct tctcgctctc
     3121 aacccagaaa cggtgagagt gcaggttcgg caggaaacgg cgtttagtcg cgttcagtgc
     3181 gtgggaacgg ttgttaccgg tcaccggacg cttgccagta acttggcaga ctcgggacat
     3241 gtctattctc caaaaatcaa attagctcga gcttcgtatg gggtattggc gcctcgtcag
     3301 gctttacagc ccggtcatcg cagttctatg tgaactctcg attgccaggc ccaaatgcca
     3361 aacccgagat tctcaaaggt ggcgtagtat acgctgactc agcgatgtgc tcaagtcccg
     3421 aacagacaaa gatcccgaag gatcgcgcat agcgggttaa atccagccgc gttcggcaaa
     3481 agaaacatac tctccacgcc caatcacgat atggtcgagc acgcgtaaat ccatgaactg
     3541 acaactcttt attatccgtt cagtaatgag tttatccgct ttactgggtt cagcacaacc
     3601 cgaagggtga ttatgtgcaa ggatcagcgc cgaggcattt atttttatcg cttcgcggat
     3661 aatttcccga ggatggactt caacatggtt tagcgtgccg gaaaaaagac ggctatgcgt
     3721 tataaccctg tgttgtgagt cgagaaagat caccataaag atctcccgct cctcacccgt
     3781 gagctggctt tgtaaaaatt cccgcgtcat ctccgggctg agtaaagggc tttcttcacg
     3841 catccgcacg ttgtagtaac gccgcgccag ttcggcaatc ccctttaact gggcaaattt
     3901 cgccacgcca attccatgaa caccactaaa ttgttcatat tcagaggtta acaagccata
     3961 aagagagccg aaattctcca gcatctcttt tgccagggtt aatacatctt taccgcgcgt
     4021 tccggtacgc agaaatagcg ccagcagctc gacatccgtt aaggcgctaa taccaaactt
     4081 cagcattttt tcgcgcggca tcaacagctg tgaattgttt ttcaccttca cctcctttgt
     4141 ggtgctcgca tcctgtcata gccgttccgt taaatcgacg gccagttttc aatcctggaa
     4201 agcgcctcgc aaagtgattc tcaggtgaag gcacgacaac aaaagtattg tgataaaatc
     4261 gccaacttct ggtgtcaccc aacaggaaaa atcatcatga gcctggccgg taaaaaaatc
     4321 gttctcggcg tcagcggcgg tattgctgcc tataaaaccc ctgaactggt gcgtcgtttg
     4381 cgcgagcgcg gggccgacgt ccgcgtagcc atgaccgaag cggcaaaagc ctttatcacc
     4441 ccacttagct tgcaggcggt ttctggttat cccgtttccg acagtctgct ggacccagca
     4501 gccgaagccg ctatgggcca tattgagctg ggtaaatggg ctgatttagt gattctcgcc
     4561 cctgccacgg cagatttgat tgcccgtgtt gctgccggaa tggcgaatga tctggtatcg
     4621 acgatttgtc tggccacacc tgcgcctgta gccgtgctcc ccgccatgaa ccagcagatg
     4681 taccgtgccg ctgccacgca gcataattta gaggtgcttg cttcccgtgg tttgctcatt
     4741 tgggggccag acagtggcag tcaggcttgt ggtgatatcg gccctgggcg aatgctcgat
     4801 ccgttaacca ttgttgatat ggcggtagcg catttttcgc ccgtcaacga cctgaaacat
     4861 ctgaacatta tgattaccgc cggcccgacg cgtgaaccac tcgatccggt gcgttatatc
     4921 tctaatcaca gctcaggcaa gatgggtttt gctatcgccg ccgccgctgc ccgtcgtggc
     4981 gcgaacgtca cgctggtatc aggtccggtt tcactaccga cgccaccgtt tgttaaacgt
     5041 gttgatgtga tgaccgcgct ggaaatggaa gccgccgtga atgcatctgt acagcagcaa
     5101 aatattttta tcggttgcgc cgccgtggcg gattatcgcg cagctaccgt ggccccagaa
     5161 aaaatcaaaa agcaggccac gcagggtgat gaattaacaa taaaaatggt taaaaacccc
     5221 gatatcgtcg caggcgttgc cgcactaaaa gaccatcgcc cctacgtcgt tggatttgcc
     5281 gccgaaacaa ataatgtgga agaatacgcc cggcaaaaac gtatccgtaa aaaccttgat
     5341 ctgatctgcg cgaacgatgt ttcccagcca actcaaggat ttaacagcga caacaacgca
     5401 ttacaccttt tctggcagga cggagataaa gtcttaccgc ttgagcgcaa agagctcctt
     5461 ggccaattat tactcgacga gatcgtgacc cgttatgatg aaaaaaatcg acgttaagat
     5521 tctggacccg cgcgttggga aggaatttcc gctcccgact tatgccacct ctggctctgc
     5581 cggacttgac ctgcgtgcct gtctcgacga cgccgtagaa ctggctccgg gtgacactac
     5641 gctggttccg accgggctgg cgattcatat tgccgatcct tcactggcgg caatgatgct
     5701 accgcgctcc ggattgggac ataagcacgg tatcgtgctt ggtaacctgg taggattgat
     5761 cgattctgac tatcagggcc agttgatgat ttccgtgtgg aaccgtggtc aggacagctt
     5821 caccattcaa cctggcgaac gcatcgccca gatgattttt gttccggtag tacaggctga
     5881 atttaatctg gtggaagatt tcgacgccac cgaccgcggt gaaggcggct ttggtcactc
     5941 tggtcgtcag taacacatac gcatccgaat aacgtcataa catagccgca aacatttcgt
     6001 ttgcggtcat agcgtgggtg ccgcctggca agtgcttatt ttcaggggta ttttgtaaca
     6061 tggcagaaaa acaaactgcg aaaaggaacc gtcgcgagga aatacttcag tctctggcgc
     6121 tgatgctgga atccagcgat ggaagccaac gtatcacgac ggcaaaactg gccgcctctg
     6181 tcggcgtttc cgaagcggca ctgtatcgcc acttccccag taagacccgc atgttcgata
     6241 gcctgattga gtttatcgaa gatagcctga ttactcgcat caatctgatt ctgaaagatg
     6301 agaaggacac cacagcgcgc ctgcgtctga ttgtgttgct gcttctcggt tttggtgagc
     6361 gtaatcctgg cctgactcgc atcctcactg gtcatgcgct aatgttcgaa caggatcgtc
     6421 tgcaagggcg catcaaccag ctgttcgagc gtattgaagc gcagttgcgt caggtattgc
     6481 gtgaaaagag aatgcgtgag ggtgaaggtt acaccaccga tgaaaccctg ctggcaagcc
     6541 agttgctggc cttctgtgaa ggtatgctgt cacgctttgt ccgcagcgag tttaaatacc
     6601 gcccgacgga tgattttgac gcccgctggc cgctaattgc cgcacagttg cagtaatatg
     6661 acgccggatg acttttcatc cggcgagttt ctttaaacgc caaactcttc gcgataggcc
     6721 ttaaccgccg ccagatgttc cgccatttct ggcttctctt ccaggtaagc aatcaggtct
     6781 ttcagggtga tgatagagat caccttgcag ttgtaatcac gctcaacttc ctgaatcgcc
     6841 gaaatctcgc cgcgcccgcg ttcctgacga tcaagcgaaa tcaatacgcc agccagcgtc
     6901 gcgccattgg cctgaataat ttccatcgac tcgcgaatcg ccgttccggc ggtgatcaca
     6961 tcatccacca gcattacgcg tccttgtaac gcgctaccga ccagattgcc accttcacca
     7021 tggtcttttg cttctttgcg gttaaagcag tacggcaggt ccaggtcatg atgctccgcc
     7081 agcgccacgg cagttgtggt ggcaatcggg atccctttgt aagcagggcc aaacagcaga
     7141 tcgaactcaa tgccggaatc caccaatgct tcagcgtaaa aacggcctaa cagtgccaga
     7201 tcgcgcccgg tattaaacag cccggcgttg aagaaatagg ggcttttgcg cccggatttc
     7261 agcgtaaact cgccaaactt taacacctgc ttgctaagcg caaattcaat aaactggcgc
     7321 tgatatggtt tcatgccttc gctcctcatc ttacttttct acagacaaaa aaaaggcgac
     7381 tcatcagtcg ccttaaaaat cagtttgcca gcgccgcctt ctgcgtcgct acaatggatt
     7441 cgattccccc tcgggccagc gccaacaagg tgagtaactc ttcgtgggtg aacggttcgc
     7501 cttctgccgt cccctgcact tcaatgatgc gcccgtcttc ggtcatcact acgttcatgt
     7561 cggtctctgc ggctgagtct tcaacgtatt ccagatcgca aaccgcttcg ccgttcacaa
     7621 ttccgacaga aactgcggct accatccctt tcatcgggtt ggttttcagc tttccgtttt
     7681 ccaccagctt ctgtagcgca tctgccagcg ccacgcaggc acccgtaatc gacgcggtac
     7741 gagtgccacc atcagcctga agcacgtcgc agtccagcgt aatggtgaac tcacccagcg
     7801 ctttcaaatc tactgccgca cgaagagcac gggcgatcag acgctggatt tccatggtgc
     7861 gtccaccctg cttacctttc gccgcttcac gcgcgttacg ggtgtgggta gaacgtggca
     7921 gcatgccgta ctctgcggtg atccagccct ggccctgacc tttcaggaag cgcggcacgc
     7981 cttcttcaat agaggcggta cacaatactt tggtatcgcc aaattcgacc agcaccgagc
     8041 cttctgcatg ttttgtatag ttacgagtca gggtaacggg acgcacctga ttattgctac
     8101 ggcctgctgg acgcatattg aaatctccgg cttgaaacaa atgtggctgc gcattatacg
     8161 gacttccggc ggttattcct atcctgacaa ggcatcgatg gctataatcc ttccacctct
     8221 ccttttataa acaggaacgt ctatgatccg cagtatgacc gcctacgccc ggcgtgaaat
     8281 caagggtgaa tgggggagcg caacctggga aatgcgctcg gtaaaccagc gttatctgga
     8341 aacttacttt cgtctgccgg agcagttccg tagccttgaa cctgtcgttc gtgagcgtat
     8401 tcgttctcgc ctgacgcgcg gtaaagtgga atgtaccctg cgctatgagc cagatgttag
     8461 cgcgcaaggt gagctgatcc tcaacgaaaa actggctaaa cagctggtaa ctgccgcgaa
     8521 ctgggtaaaa atgcagagtg acgaagggga aatcaacccg gttgatattc tacgctggcc
     8581 gggcgtgatg gcagcccagg agcaggatct cgacgcgatt gccgctgaaa ttctcgcggc
     8641 gttggatggt acgctggacg actttatcgt cgcgcgcgaa accgaaggtc aggcgctgaa
     8701 agcattgatc gagcagcgtc tggaaggcgt caccgccgaa gtggtcaaag tccgcgccca
     8761 tatgccggaa atcctgcaat ggcagcgtga gcgtctggtc gcgaagctgg aagatgctca
     8821 ggtgcaactg gaaaacaacc gtctggagca ggagttggtt ctgctggcac aacgaattga
     8881 cgttgccgaa gaactggacc gcctcgaagc gcatgtcaaa gagacctaca acattctgaa
     8941 gaaaaaagaa gcggttggtc gtcgtctgga ctttatgatg caggagttca accgcgagtc
     9001 gaacactctt gcgtcgaaat ctatcaatgc cgaagtgacg aattctgcca tcgagctgaa
     9061 agtgttgatt gagcagatgc gcgagcagat ccaaaatgtg gagtaagcta tcagaggaaa
     9121 tcaataaagc gcaataatcc aatcaaaccc gcgataatgc gggttttttt gtaattaatg
     9181 actatcataa cgacgcaaga aagagcatga ttagcgtgta cataaaggtg tacataatgc
     9241 catttatgta catcgcttta tgtacacacc tctattcaat gtttagggat gccttaatgc
     9301 caaaacttac agatattcag atccgtgcct ggataaaggc agacgagagg tttgacggca
     9361 aagctgacgg taacggcctg tatctctgct atcccaaaaa ttacacaatg cccttctggc
     9421 gattccgcta taagtttgct ggcaaacaac gggcgatggt tatcgggtca tatgcagagt
     9481 tatcactatc cagagccaga gaaacagcaa aggaactatc cgctcgcgtc gcacttgggt
     9541 acgacgtagc agcggagaaa caggagcgta aagccgaagc actggcaaaa atggaggcag
     9601 aaaaaaacgc catacgagta tcagagcttg ctgccgaata tttcgaacgt cagatactgc
     9661 cacgatggaa acatcccgac atcctgcgcc gtcgcatcga caaagacata aacccgcgta
     9721 ttggtcatat gaaggctgag gacgtaaaac cacgacacat tgacgagatg ctaaaggcaa
     9781 ttgttgaccg tggagcgcca accattgcaa cggacgttct gcgatggacg cgccgcatat
     9841 tcgactacgg gataaaacgt catgtgctgg agattaaccc gtgctcggcg tttgaagtct
     9901 ctgatgctgg tggcaaggag tcatcccgcg atcgctggct gacccgtgaa gagttaatcc
     9961 ggttctttca ggccatgcgt accgcaaagg gattcagccg acagaacgag ctaaccttca
    10021 agttgctact ggcgctttgc gtcaggaaga tggagttgtg cgccgccaga tgggaagagt
    10081 tcgaccttga taacgccgtg tggcatttgc cagaggaacg cagcaaaaac ggcgacccca
    10141 tagatattcc acttgttccc cccgttattg aatggctgaa agaactgcgc gtcttgtcat
    10201 gcaatagcgc gtgggtactt ccggcgcgga aaatgcagca caggatgatc ccccacattc
    10261 aggagagcac cctacccgtt gcactggcaa aggtccgcgc agagatgccg gaggttccag
    10321 atttcactat tcacgacttc cgacgtacag cacgcacaca tttagccgcg cttggcgttg
    10381 atccggtagt cgctgaaaga tgcctcaatc accgcatcaa gggagtcgag ggcatataca
    10441 atcgctatca gtatttcgat gaacggactt cggcgctggc gaaatggacc aatctgttag
    10501 tgtcgctgga acgcggggaa cgctacaacg tgacaccaat cagaagggcc aaagcatgat
    10561 taatatcctg aaaaaatcgc caatagaacg cctgtgcgct tctgtttcaa tcactccgca
    10621 cgaaatggcc cttgcactgg ctggccttaa tccgtcaatg agaataggcg acgtgccgca
    10681 ggacaagttc gaacaggtcg aatcagccag aacgatgata gcccgtgcta tctggttgca
    10741 tagcggcaag aaagcgggaa aggatgagcc atacagagcg ggggatatat tccttgcgtc
    10801 cttcccgttc attgaggcag gaacgccaga ggcaataatt acggcggtaa ctgatgccat
    10861 tgatgattta cgaggaacaa aaaattggga agaaaaagca cttaatcttg gtgggcggcg
    10921 gctggtcagc cacatcaaag aaacaagccg cagcggaagg gggcagtaca ggaaactcga
    10981 cgaagaacag ggaaacatga agatgatggg gttgctggtt ttactgctcg tgaagaaatc
    11041 aggcacaacc gcatacattc aggacggaga accaaaccgt tcagccattt accgcgacgt
    11101 ggaagcgctg atgaaggaga aagggatatc gccaaaaggg atcgccaaat ccacgtttat
    11161 gcaaaaaata tcagccgcat tactggcagt aagccaggca gattaaccag ccaacacgcc
    11221 ctgtattcac ggggctgtaa tccccataca tcccccacca gcaaaattta ctcaccgttc
    11281 ttaatcgtta taaatcggta taaacaggat tagcacgtta aagactggca aaagatggca
    11341 aaacataaca acgcataacg gcgcagtagg agtattacgg tatcagaacg tatacggctt
    11401 caatgactta tcaccaataa agcaaaaact ttcaaagttt tagcactcca tcccgcctgt
    11461 tcaatctttt actggaagtg tgttttttca atagatttgt ggaaagtttt aatagattcc
    11521 agatttggtg gaatgctgta caaacaacca atagaatgga gaaaacatca acttttaacc
    11581 caagagggaa tcatgacagc taaccatcaa gaagctatcc ctaccacggg ctatatccgc
    11641 cgctgcaacc tggctaagtt gcttgatacc agcgtgtcaa ctatcgaccg ctgggtgcgt
    11701 atcggcaaat taccgaagcc cacgaagctg ggggagaaaa acaccgtatt taatgcggtg
    11761 gagatcaacc actggctgaa cgagcgccgc aacaacacca agcagtaatc agcatgacgg
    11821 gcgtacagtt ccgcgcccg
//
LOCUS       DAZFLD010000144        11782 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.145, whole genome shotgun sequence.
ACCESSION   DAZFLD010000144 DAZFLD010000000
VERSION     DAZFLD010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11782
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.145"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..9565)
                     /gene="cdiA"
                     /locus_tag="ACHKBL_003439"
     CDS             complement(<1..9565)
                     /gene="cdiA"
                     /locus_tag="ACHKBL_003439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001385946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="contact-dependent inhibition toxin CdiA"
                     /protein_id="HFO5820265.1"
                     /translation="MHQPPVRFTYRLLSYLISTIIAGQPLLPAVGAVITPQNGAGMDK
                     AANGVPVVNIATPNGAGISHNRFTDYNVGKEGLILNNATGKLNPTQLGGLIQNNPNLK
                     AGGEAKGIINEVTGGNRSLLQGYTEVAGKAANVMVANPYGITCDGCGFINTPHATLTT
                     GKPVMNADGSLQALEVTEGSITINGAGLDGTWSDAVSIIARATEVNAALHAKDLTVTA
                     GANRITADGRVSALKGEGNVPKVAVDTGALGGMYARRIHLTSTESGVGVNLGNLYARE
                     GDIILSSSGKLVLKNSLAGGSTTVTGTDVSLSGDNKAGGNLSVTGTTGLTLNQSRLVT
                     DKNLVLSSSGQIVQNGGELTAGQNAMLSALHLNQTSGAVNAAENVTLTTTDDTTLKGR
                     SVAGKTLTVSSGSLNNGGTLVAGRDATVKTGTFSNTGAVQGNGLKVTATDLTSTGSIK
                     SGSTLDISARNATLSGDAGAKDSARVTVSGTLENRGRLISDDVLTLSATQINNSGTLS
                     GAKELVASADTLTTTEKSVTNSDGNLMLNSASSTLAGETSAGGTVSVKGNSLKTTTTA
                     QTQGNSVSVDVQNAQLDGTQAARDILTLNASEKLTHSGKSSAPSLSLSAPELTSSGVL
                     VASALNTQSQTLTNSGLLQGEASLTVNTQRLDNQQNGTLYSAADLTLDIPDIRNSGLI
                     TGDNGLTLNTASLSNPGKITADTLNVRATTLDGDGLLQGAAALALAGDTLSQGRHGRW
                     LTAGDLSLRGKTLNTAGTTQGQNLTVQADRWANRGSVLATGNLTASATGQLTSTGDIM
                     SQGDTTLNAATTDNRGSLLSAGTLSLDGNSLDNSGTVQGNHVTLHHRSTDNSGTVTGL
                     SGLTLHSADGLTNSGALLSQNSLVLSAGDVTNSGRIQGQNITLDASSLTSSGAVQSAL
                     DLALTLSGDVIAATGSKITALGDARLSGKVLGNQGLISAKTLEVNGDSLSNSGEISGV
                     NSLNVTLSGNLQQHGKMLTGGALNVNARDISNSGQLQGADNRITASSLANSGRVQGES
                     GLTLTLLNALTNQTSGVLLSQNVSALSAPALTNDGTIQGNGKTTLSAATQAHNSGKIL
                     SGGELTFTTPDYSGSGWLQATDLLLNVAKLAGNGTVMAANQATLTGNSLTNRGLFQAA
                     QLNVNTQTITNSGTLLGNQGLTIKGNNLNNAGGKVFSGGDMLAEMVSLSGAGQLVALG
                     NLTLKLTRGLTAQGVIAANKQLSVSSQGDITNGATLQGNGITLNAAGRLTNNGQLTAG
                     NGTTALSGSGIAMNASGSLQAGGDVSLTSRGDITLDAFTGTTGSLMLTAAGAVINTAL
                     LYAGNNLSLFASTIRNHHGDMLAGDSLVMQKDVSGAANAEVINTSGNIETTRGDITIR
                     TGHLLNQREGINETKSYIPVENVAVPDGANSVSVRVGDLGEDGWGYYVKSWSGTAGGG
                     FDAWAVPTEKGATRKFLTGTTRVDVGATGGDARISAGNNLLIDADKLDNTGSHLLASG
                     FVSLSGSQLNNQSFFGYTQDEYNVYRYYGKLAMIPNDGHLQYGDASADDRVTFTLSGA
                     PEYVTRDTGQALRAVIQAGKNVTAVFSSDISNTSTTSNAGRITNTLAAPEINTPAEKN
                     ISPGMAQLAPDGTETLTVTAPDWTDTITRLTIGSGTDLASGIVEGNYPLPSGNNGYFV
                     PSTDPDSPYLITVNPKLDGLGKVDSSLFTGLYDLLRMHPGQAPRETDPAYTDEKQFPG
                     SSYFLDRLGLKPEKDYRFLGDAAFDTRYVSNYMLNQIGGRYINGVGSDTDQMRYLMDN
                     AARAQKALGLKFGVALTADQVAALDQSILWYKAVTIKGQTVMVPEVYLSPKDVTLQNG
                     SIISGQNVHLAGGNVTNSGSTLMAQNNLTIDSADSLGNLESGLINAGGALGLKAMGDI
                     NNISATITGKTVRLESLAGNVNNLTRYSHWQLDAPEDSLALKHTYTGSIASVSAMDSL
                     DIRADKNISVTGAEISAGDRAALIAGNDLSLNAIDRVSSRRHANSESHQRSAGLTTIT
                     AGDSVMLSAGRDVSSQGAGIAAEDNITVRAGRDVNLLAEESVTGSSSYSKKKTVIDET
                     VRQQGTEIASGGDTTITAGRDITAVASSVTATGNISVNAGRDVALTTATESDYHYLET
                     KKKSGGFLSKKTTHTISEDSATREAGSLLSGNRVTVNAGDNLTVEGSDVVADRDVSLA
                     AGNHVDVLAVTSTDTSWRFKETKKSGLMGTGGIGFTIGSSKTTHDRREAGTTQSQSAS
                     TIGSTAGNVSITAGKQAHISGSDVIANRDISITGDSVVVDPGHDRRTVDEKFEQKKSG
                     LTVALSGTVGSAINNAVTSAQETKESSDSRLKALQATKTALSGVQAGQAAAMATATGD
                     PNATGVSLSLTTQKSKSQQHSESDTVSGSTLNAGNNLSVVATGKNRGDNRGDIVIAGS
                     QLKAGGNTSLDAANDILLSGAANTQKTTGRNSSSGGGVGVSIGAGGNGAGISVFASVN
                     AAKGSEKGNGTEWTETTTDSGKTVTINSGRDTVLNGAQVNGNRIIADVGHDLLISSQQ
                     DTSKYDSKQTSVAAGGSFTFGSMTGSGYIAASRDKMKSRFDSVAEQTGMFAGDGGFDI
                     TVGRHTQLDGAVIASTATPDKNHLDTGTLGFSDLHNEADYKVSHSGISLSGGGSFGDK
                     FQGNMPGGMISAGGHSGHAEGTTQAAVAEGTITIRDRDNQKQNPADLSRDPAHANDSI
                     SPIFDKEKEQRRLQTVGLISDIGSQVADIARTQGELNALKAAKEATGETLPANATEKQ
                     RQEYLAKLRDTPEYKKEQEKYGTGSEIQRGIQAATAALQGLAGGNLAGALAGASAPEL
                     AHLLKSTEKDPVVNAIAHAILGGAVAAMQGNNVAAGAAGAATGEMAARAIAGMLYPGV
                     KQSDLSEEQKQTISTLATVSAGLAGGLTGNSSASAAVGAQSGKNAVDNNYLSVSEKTE
                     LEIAKQTLKNSKNPAEREKAQQKYDALLEKDIASDKEVIAACGNGNAGSSACASARLK
                     VIASKEGYEDGPYNSKYSQQYADAYGQIVNLLDITSVDVQNQQQVKDAMVSYFMATLG
                     VDQKTAQGYVETTQGLEIAAASMTPLFGQAVANKITALVDKANKYPSGIGFKINQPEH
                     LAQLDGYSQKKGISGAHNADVFNKAVVDNGVKIISETPTGVRGITQVQYEIPTKDAAG
                     NTTGNYKGNGAK"
     gene            complement(9578..11356)
                     /locus_tag="ACHKBL_003440"
                     /pseudo
     CDS             complement(9578..11356)
                     /locus_tag="ACHKBL_003440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020231833.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0046930 - pore complex [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
BASE COUNT         2374 a         3450 c         3021 g         2937 t
ORIGIN      
        1 gcttggcgcc atttccttta taattaccag tcgtattccc agcagcatct tttgtgggga
       61 tttcatactg aacctgagta atacctctga ctcctgttgg tgtttcagaa ataattttca
      121 caccattatc aaccacagct ttattgaata catcagcatt atgtgcaccg ctaattcctt
      181 tcttctgcga atatccgtca agttgagcta agtgttccgg ctgattaatt ttaaaaccaa
      241 tccctgaagg atatttattc gctttatcaa ccaatgcagt aattttatta gccacagcct
      301 gaccaaataa tggtgtcatt gaagccgctg caatttctaa tccctgcgtc gtctcgacat
      361 aaccttgtgc tgttttctgg tcaaccccca gcgttgccat gaagtaactc accatcgcat
      421 ctttcacctg ctgttgattt tgtacatcaa cactggtgat atcgaggaga ttgactatct
      481 gaccatacgc atcagcgtat tgctggctgt atttcgagtt ataagggcca tcttcgtacc
      541 cttctttcga cgcaataact ttcagtcttg cgctggcaca cgctgaacta cccgcattac
      601 cattaccgca ggcagcaata acctccttat cactggcaat atctttttca agcaatgcat
      661 catatttctg ctgtgccttt tcacgctctg ccggattttt actgttttta agcgtctgtt
      721 ttgctatctc aagctctgtc ttttcagaca cgctcaggta gttattatca acagcattct
      781 tcccgctctg tgcgcccact gctgctgatg cgctgctgtt tccggtaaga ccgccagcca
      841 gccctgctga cacggttgcc agcgtactta tcgtctgctt ctgctcttca ctgaggtctg
      901 actgtttcac gcccggatac agcatgccgg caatcgctct ggctgccatc tctcccgttg
      961 ctgcgcctgc tgcaccggca gcaacattat taccctgcat tgccgctact gcgccaccca
     1021 gaatggcgtg agcaatggca tttactaccg gatctttttc tgttgatttc agcaggtgcg
     1081 ccagttccgg ggccgaagct ccggccagag cacccgctaa attcccccca gccagtccct
     1141 gaagcgcggc tgtcgcggcc tgtatacccc gctgaatctc actgccagtg ccatactttt
     1201 cctgttcctt tttgtattcc ggcgtatcac gcagtttcgc aagatattcc tgacgttgtt
     1261 tctctgttgc attagcagga agtgtttcgc cggttgcttc cttcgcggcc ttcaacgcat
     1321 tcagttcccc ctgcgtccgc gcgatatccg ccacctgact gcctatgtca ctgataagcc
     1381 ccactgtctg cagacgcctc tgctccttct ccttgtcaaa tatcgggctg atactgtcat
     1441 tagcgtgcgc aggatcacgg ctgaggtctg ccggattctg cttctgattg tccctgtccc
     1501 ggatggtgat agtgccttct gccactgcgg cctgagtcgt tccttccgca tgtccgctgt
     1561 gacctccggc ggatatcatg ccacccggca tgttaccctg aaatttatcc ccgaagctgc
     1621 caccaccgct cagactgatc ccactgtgac tgactttata atccgcttcg ttgtgaaggt
     1681 cactgaaccc cagcgttccg gtatccaggt ggtttttatc cggtgtggca gtggaggcaa
     1741 tcaccgcacc atccagttgg gtatgtctgc ccactgtgat gtcgaagcca ccatcaccgg
     1801 caaacattcc ggtttgttca gcaacggagt caaagcggct cttcatctta tcccgggagg
     1861 cagcgatgta acctgagccg gtcatggagc caaaggtaaa actgccgccg gcagccacgc
     1921 tggtctgttt actgtcgtac ttactggtgt cctgctggct gctaatcagc aggtcgtggc
     1981 ccacatcggc gataatcctg ttgccgttga cctgagcacc gttcagtacc gtatcccgac
     2041 cactgttgat ggtgacggtt ttaccgctgt ctgttgtggt ttcagtccac tcagtaccgt
     2101 tacctttctc gctgcctttt gccgcattaa cgctggcaaa gacgctgata ccggcaccgt
     2161 taccaccggc accgatactg acacccacgc caccgccact gctgctgttc ctgcccgttg
     2221 ttttttgtgt gtttgcagcg ccactcaaca gaatatcatt cgcggcatcc aggcttgtgt
     2281 taccaccggc cttaagctgg cttcctgcaa tcacaatatc tccgcggtta tcgcccctgt
     2341 ttttgccggt tgcgacaaca gacagattat tcccggcatt cagcgtactg ccggatactg
     2401 tgtcactttc agaatgttgt tgtgatttcg atttctgagt ggtgagcgac aggctgactc
     2461 ccgtcgcatt cgggtcaccg gttgcggtgg ccattgccgc agcctgtccg gcctgcacac
     2521 cagacagcgc tgtctttgtg gcctgcaggg ctttcagacg actgtcactg ctctccttcg
     2581 tttcctgtgc actggtgacc gcattattga tggcactgcc cactgtgccg gaaagggcaa
     2641 ccgtcagccc gcttttcttc tgctcaaatt tttcgtccac agtacgacgg tcatgccccg
     2701 ggtcaaccac cacactgtca ccggtaatgc tgatatcccg gttcgcaatc acatccgaac
     2761 cgctgatatg agcctgtttg cccgcggtaa tactgacatt accggcagtg gagccgatgg
     2821 tactggcact ctgactctgc gttgtcccgg cctcgcggcg gtcgtgcgtt gtcttactgc
     2881 tgccgatggt gaagccgata ccgccagtac ccatcagacc cgatttcttc gtttccttaa
     2941 agcgccagga cgtatctgta ctggtgacag caagaacatc aacatggtta cccgccgcca
     3001 gtgacacatc ccggtcagcc accacatccg aaccctctac cgtcaggtta tcaccggcgt
     3061 taacggtcac gcggttcccc gacagcaggg agcctgcttc acgggtggca ctgtcctcac
     3121 tgatggtgtg ggtggttttc ttactgagaa aacctccgct ttttttcttc gtttccagat
     3181 agtgatagtc actttctgtc gccgtggtca gggcaacatc acgaccggca ttcacgctga
     3241 tattgccggt tgcggtaacg gatgacgcaa cagcggtgat atcccgtcct gcagtaatgg
     3301 tggtgtcacc accgctggcg atttccgttc cctgctggcg aacggtttca tcaatgaccg
     3361 ttttcttttt cgagtatgaa ctgctgccgg tcacggactc ttccgccagc agattaacgt
     3421 cgcgcccggc ccggacagtg atgttgtctt ccgctgcgat acctgcaccc tgactgctca
     3481 catcccggcc tgcactcagc atcacactgt cgccggcagt aatggtcgta agcccggcgc
     3541 tgcgctggtg agactcactg tttgcatgac gtctgctgct taccctgtca atggcattca
     3601 gactgagatc attgccggca ataagtgctg cgcggtctcc ggcactgatt tctgcccctg
     3661 tgaccgaaat gtttttatct gcccggatgt ccagactatc catggcactg acagaggcaa
     3721 tgcttcccgt ataagtgtgt ttcagggcga ggctgtcttc cggtgcatca agctgccagt
     3781 gtgagtaccg ggtgagatta ttgacattgc cggcgagact ctccagccgg acagtcttac
     3841 cggtaatcgt ggcactgata ttgttgatat cgcccatcgc tttcagtccg agagcgccac
     3901 cggcatttat aagcccggac tcaaggttac ccagactgtc agcgctgtcg atggtgagat
     3961 tattctgcgc catcagggta ctgccactgt tagtgacatt ccctcctgcc aggtgcacat
     4021 tctgaccgct gataatactg ccgttctgca gcgtcacatc tttcggtgac agatacacct
     4081 ccggcaccat caccgtctga ccctttatcg tgacggcttt ataccacaga atactttggt
     4141 cgagagccgc cacctggtct gctgtcagtg ccacaccaaa cttcagaccg agcgccttct
     4201 gtgcacgggc agcgttatcc atcaggtagc gcatctggtc ggtgtctgaa ccaaccccgt
     4261 tgatatagcg ccccccgatc tggttcagca tatagttcga tacatagcgg gtatcaaaag
     4321 cggcatcccc caggaagcga taatcttttt ccggtttcag cccgagccgg tcaagaaaat
     4381 acgatgagcc cgggaattgt ttttcatcag tatacgccgg gtctgtttca cgtggtgcct
     4441 gcccggggtg catcctgagc aggtcataca gtccggtaaa cagactgctg tccacctttc
     4501 cgagaccatc cagtttcgga ttcaccgtaa tcagatatgg actgtcaggg tccgtggacg
     4561 ggacaaaata accattgtta ccggaaggca ggggatagtt cccctccacg atgccggaag
     4621 ccaggtccgt gccactgccg atcgtcagac gggtaatggt gtcagtccag tccggggctg
     4681 tgacggtcag cgtctctgtg ccatccggcg ccagttgagc cattcccggg ctgatatttt
     4741 tctctgccgg cgtgttaatt tcaggtgccg caagggtatt tgtgatccgt cctgcatttg
     4801 aggtggtgga tgtattactg atatcactgg aaaaaacggc agtgacattt ttgcctgcct
     4861 gaatcacggc tctcagagcc tgaccggtgt ctctggtgac atattccgga gctccggaga
     4921 gagtaaaggt aacgcgatca tcggcagaag catctccata ctgcagatgc ccgtcgttgg
     4981 ggatcatggc cagtttgccg taatagcgat acacattgta ttcatcctgt gtatagccaa
     5041 agaaggactg attattcagt tgtgaacccg acaggctgac aaacccgctg gcaagaagat
     5101 gacttcccgt attatccagt ttgtccgcgt caatcagcag attattcccg gccgaaattc
     5161 tggcatcccc ccctgttgca ccaacatcga cgcgtgttgt gccagtcaga aatttccggg
     5221 tggctccctt ttcagtcgga acggcccatg cgtcaaaacc accaccggca gtaccgctcc
     5281 aggacttcac ataatatccc catccatctt cacctaaatc gcccactctg actgaaacag
     5341 aatttgcacc atcaggtact gcgacattct caaccggaat gtatgatttt gtctcattta
     5401 tgccctcccg ctgattcagt aaatgtccgg ttctgatggt gatatcaccg cgggtggttt
     5461 cgatattccc ggaggtatta atcacttcag cattcgcggc accactgaca tctttctgca
     5521 tcaccaggct gtcacccgcc agcatatcgc cgtgatggtt ccggatggtg ctggcaaaca
     5581 gcgagaggtt attgcccgca tacaacagtg cggtgttaat gactgccccg gcggccgtta
     5641 acatcagact acccgtcgtg ccggtaaaag cgtcgagagt gatatctccc ctgctggtca
     5701 ggctgacgtc cccgcctgcc tgcaggctgc cgctggcatt catcgcaatc ccgctgccgc
     5761 tcagtgccgt ggtgccgttg ccggcggtca gctggccatt gttggtcagt ctgcctgcag
     5821 cattcagcgt gataccgttg ccctgcaatg tggcgccatt ggtaatatcc ccctggctgc
     5881 tgacggagag ttgtttattt gcggcgataa cgccctgtgc ggtcaaacca cgggtgagct
     5941 tcagcgtcag gttaccgagt gcaaccagct ggcccgcgcc gctgagcgaa accatttcag
     6001 caagcatatc accgccgctg aacaccttcc cgccggcgtt gttcaggttg ttccctttta
     6061 ttgtcaggcc ctgattaccc agcagcgtgc cgctgttggt gatggtctgc gtgttgacgt
     6121 tcagttgtgc ggcctggaac agaccacggt tggtcagact gttacctgtg agcgttgcct
     6181 gattcgccgc catcacagtg ccattaccgg caagcttcgc cacgtttagc agcaggtcag
     6241 tggcctgtag ccagccactg ccactgtagt ccggcgtggt gaacgtcagc tcaccgccgg
     6301 aaagaatctt gccactgttg tgggcctgcg ttgccgcgct gagcgtggtt ttaccgttgc
     6361 cctgaatggt gccgtcattg gtcaacgcgg gtgcggaaag cgctgatacg ttctggctga
     6421 gaagcactcc gctggtctgg ttggtcaggg cattcagcaa tgtaagagtc agcccgctct
     6481 cgccctgaac acgacctgag ttggccagcg aactggcggt gattcggtta tccgccccct
     6541 gaagctgccc actattgctg atgtctctgg cgttgacgtt taatgcaccg cccgtcagca
     6601 ttttaccgtg ctgttgcaga ttaccactga gtgtcacgtt caggctgtta accccactga
     6661 tttcgccact gttgctgagc gaatcgccat tcacttccag cgtcttcgcg ctgattaatc
     6721 cctggttacc cagcactttg ccgctcagac gggcatcgcc cagggcggta attttgctgc
     6781 cggtggcggc aataacatcg ccactcaggg tcagagccaa atccagcgca ctctgaacag
     6841 caccgctgct ggtcaggctg ctggcatcca gagtgatgtt ctgcccctga atccggccgc
     6901 tgttggtcac atctcctgcg ctcagcacca gcgagttctg gctcagcagc gcgccactgt
     6961 tggtaagccc gtccgcgctg tgcagggtca gaccgctcag ccccgtgacc gtaccgctgt
     7021 tgtcagtgct tctgtggtga agcgtgacat ggttaccctg gacagtgccg ctgttatcca
     7081 gtgagtttcc atcaagggag agcgtgccgg ccgaaagcag actgccccgg ttgtccgtgg
     7141 tggctgcgtt cagcgtggtg tcaccctggc tcatgatatc gccggtactg gtcaactgac
     7201 cggttgccga agcagtaagg ttaccggttg ccagcacgga accacggttc gcccatctgt
     7261 ccgcctgcac ggtgagattc tgtccctgcg tggtccctgc ggtattcagt gttttacccc
     7321 ggagggagag gtcgcccgcc gtcagccagc gtccgtgacg accctgtgag agggtgtcgc
     7381 ctgcaagcgc cagtgcagcg gcgccctgca acaggccatc gccatccagc gtggtcgccc
     7441 tgacgttcag cgtgtcagcg gtgattttac ccggattgct gagggaggca gtgtttaacg
     7501 ttaaaccatt atcaccggtg ataagcccgc tgttgcggat gtccggtata tccagcgtca
     7561 ggtctgcagc actgtacagc gtgccgttct gctgattatc aagcctctgt gtgttaacgg
     7621 tgagtgaggc ctccccctgc aacagaccgc tgttggtcag ggtctgtgac tgtgtattca
     7681 gggcggaagc aacaagtacg ccgctgctgg tcagttccgg cgcactgagg ctgagcgacg
     7741 gggcgctgct tttcccgctg tgggtgagct tttcactggc gttcagggtg aggatatctc
     7801 tggcagcctg tgttccgtca agctgtgcgt tctgcacatc cacgctgaca ctgttgccct
     7861 gcgtctgcgc agtggtcgtg gtcttcagac tgttgccttt tacagacacc gtgccacccg
     7921 cactggtttc acccgccagt gtggaagacg cgctgttcag catgaggtta ccgtcactgt
     7981 ttgtgaccga tttttctgtg gtggtcagtg tgtctgcaga agccacaagt tcctttgccc
     8041 cggagagggt accgctgttg tttatctgcg tggcactgag cgtcagcacg tcatcgctga
     8101 tgagtctgcc gcggttttcg agtgtaccgc tgacggtaac gcgggcactg tcttttgcac
     8161 cggcatcacc ggacagtgtg gcattgcggg cgctgatatc gagtgtgctg ccacttttaa
     8221 tactgccggt gctggtcagg tcagtggctg tgactttcag gccgttcccc tggacggcac
     8281 cggtattact gaatgtcccg gttttcaccg tggcatcgcg cccggcaacc agtgtcccgc
     8341 cgttgttcag gctgccggag ctgacggtga gtgttttccc ggcaacgcta cggcctttca
     8401 gtgtggtatc atcggtggtg gtaagggtga cattttcagc tgcattcacg gccccggaag
     8461 tctggttcag gtgcagtgca ctgagcatgg cgttctgtcc ggcagtcagt tcaccaccgt
     8521 tctgtacaat ctgcccggat gaagacagca ccagattttt atccgtcacc agacgggact
     8581 gattcagtgt cagtcccgtt gtcccggtaa cgctgagatt tcctccggct ttgttatccc
     8641 ctgaaagtga gacatccgtt ccggttacgg tggtactgcc gccggcaagg ctgttcttca
     8701 ggaccagttt tccggaactg ctcagtatga tatcgccctc gcgggcataa aggttaccaa
     8761 gattaacccc gacaccactt tcagtggagg tcagatgaat acgcctggcg tacattccac
     8821 cgagagcgcc ggtatcaacg gcaactttcg gcacattacc ttcgcccttc agggcactga
     8881 cgcgaccatc tgcagttatc cggttagcgc ctgcagtgac agttaaatcc ttcgcatgaa
     8941 gcgcggcatt cacttccgtt gcacgggcaa taatggatac ggcatcgctc caggtgccgt
     9001 ccaggcccgc gccattgatg gtgatactgc cttcagtcac ctccagcgcc tgcaggctgc
     9061 cgtcggcatt catcacaggt ttgcctgtgg tgagcgtcgc gtgcggcgtg ttgataaaac
     9121 cacagccgtc acaggtgata ccatacgggt tggcaaccat cacattcgcc gctttgccgg
     9181 ccacttccgt atagccctgc agcaatgaac ggttaccgcc agtcacttcg ttgatgatac
     9241 ccttcgcttc cccgcccgct ttcaggttcg ggttattctg tatcagtcca ccaagctgcg
     9301 tcggattaag cttaccggtg gcattattga gaatcagccc ttccttcccg acgttgtaat
     9361 ccgtaaaccg gttatgcgaa atcccggccc cgttcggcgt ggcaatgttc acgaccggca
     9421 caccatttgc cgctttatcc attccggccc cgttttgtgg ggtgatgacg gcccccacag
     9481 ccggtaacaa cggctgcccg gcgataatcg tactgataag gtaactcagc aggcggtaag
     9541 tgaagcgaac gggaggctga tgcatggtaa taatccctta aaacgcgacg gcaacgcgcc
     9601 agtaaaccgt gagatggtca ggggcaagcc agtccgggta aaccagaggc agtccggcag
     9661 tgaacgaacc ggaaacatgg ccactggtgg tgctgagccc ggcagcagca ccccacagcg
     9721 tgccggacga gtacaggtca tctctgtcag agtgcagcca gccgccgtcc agtgcagtca
     9781 ctgcacggac tgtccccaca tatggcaggg agaacagaga ccaggacagc tcatttcgca
     9841 gataaccgcc gttattaccg gagatatact gctccttaaa gccacgcact gaactctcac
     9901 ccccgaggct cagttgttcc acaccatgaa gacggtccgg tgaccactgg gcataagcgc
     9961 tggtcagcca ccacaccctg tccgtgacgg ggcgctgaaa actggcgctt aatgaccatt
    10021 tccggaactg atttacgggc aggtctcccc ttttcccgtg gtcgctttct gcgccgaacc
    10081 agggcatccc ccgtgtgaat accggattca gtgttccgac accccccaga aacttgtgtg
    10141 tgtgattcag cccgacagaa aatgaagtga gtttacggct gctgccctga agcagaacat
    10201 catccagata attgtgaata atgcggtgct gcagacctcc ggtcagtgct gtcttcatgt
    10261 ccccgttacg gaacaggaca tgcgacagtc ccagccggtg agtctgcagg tctcccgtgg
    10321 aacgccaccg ccagccccgg ttatcaatgg tgctgaggta gtcactccat gaatacgtgt
    10381 aatccaccag ggtatagcca tacggcagac tgacaccggc ggcaaaattc ctcgcatcat
    10441 gtgacaccga aaagtcactg ctccgtcccc cgctgacaaa ccagttgtca gccagcccca
    10501 gcggattatt aaaggaaagg acaccattta actgccccgt accggtattc ttctgtccgc
    10561 tgttgtcgat gcccacgctc cctgtgacag gccattccgg cgatgccgtc agtgtcacca
    10621 ccgaccatcc ctcacggtca ccgggcgata tttaaatctg taccggctcc gtacgcagac
    10681 ggttaatctg ctccatcccc tgctcaatat cccgcaggtt cagaaccttc ccctccattc
    10741 ccgggaaaac catcttcagg gtgcggccag gaaggtcagc gccttccgcc cggatttgct
    10801 gcagcctgcc ttccatgacc gttatgtgca gtacgccccc tgaaaggtcc tgctctgtca
    10861 gaaaggcccg gctggtgata tatccccggc gtatatagcc gtccgtcacg gcatccgtga
    10921 ccgcggtcag ccccgtgata ttcagacact gattcaccca cggtgccacc agtctgtcgg
    10981 tttctgcaga cgtcagtcgg gtggccccac tgacaacaat gcgtgacacc gtaaagcagg
    11041 gaccttcagt accggcagac atttccggag acggtatgac agtcagcggc gcactgcgct
    11101 ccagcgcatc acgctgacgc tggttttcat ccagcaactg ctgctgttgc tgctgaattg
    11161 cactgcggtc acccggagac agcatggctg ccggagacga aaatgcgggg ataaaaaaac
    11221 cagaaaaaac ggagagggag agataacata tccccgaaga ggcgcgggcg cgccggaata
    11281 tatcataaca ggggacagac acagcccgga ataaaagaga cgttttcgtc agaatgttgt
    11341 tctgccggtg ctgcatgttc tccccgtatt ctggcctgtg taatgtcagt gtaaattcat
    11401 gtcaaaaatg tgtcagcggg aaatttacac ctgaaaaagt ctgtcatgaa acagctttat
    11461 gacaagatat caaaaaaaat gtcaaaaacg tgatctttct tcagcttttc agaatattga
    11521 gagcctagag gtcaatataa attctgggac acatatgcat ttaccattaa aaccggcaca
    11581 acaggctgtt cagtctcaat ccatgctttc tgttttatcg atctcagaat atcggcagac
    11641 aaataaaaaa ggaatgtcga gagagctaac tctagatcac tctccacctc acaagccatt
    11701 tacaaaaatg atacaggcat atctttggtc cctgccacaa ccagacattg acaatccaat
    11761 ataaccccaa atatatttcg ga
//
LOCUS       DAZFLD010000145        11781 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.146, whole genome shotgun sequence.
ACCESSION   DAZFLD010000145 DAZFLD010000000
VERSION     DAZFLD010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11781
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.146"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(62..343)
                     /gene="yehE"
                     /locus_tag="ACHKBL_003441"
     CDS             complement(62..343)
                     /gene="yehE"
                     /locus_tag="ACHKBL_003441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehE family protein"
                     /protein_id="HFO5820266.1"
                     /translation="MNKYWLSGIIFLAYGLASPAFSSETATLTINGRISPPTCSMAMV
                     NGQPQQHCGQLTYNVDTRHQVSSPVKGVTTEVIAADSDSKRRIVLNRYD"
     gene            complement(606..1715)
                     /gene="apbC"
                     /locus_tag="ACHKBL_003442"
     CDS             complement(606..1715)
                     /gene="apbC"
                     /locus_tag="ACHKBL_003442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HFO5820267.1"
                     /translation="MNEQSQAKSPEALRAMVAGTLANFQHPTLKHNLTTLKALHHVAW
                     MDDTLHVELVMPFVWHSAFEELKEQCSAELLRITGAKAIDWKLSHNIATLKRVKNQPG
                     INGVKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPNMLGAENQ
                     RPTSPDGTHMAPIMSHGLATNSIGYLVTDDNAMVWRGPMASKALMQMLQETLWPDLDY
                     LVLDMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVE
                     NMSVHICSNCGHHEPIFGTGGAEKLAEKYHTQLLGQMPLHISLREDLDKGTPTVISRP
                     ESEFTAIYRQLADRVAAQLYWQGEVIPGEISFRAV"
     gene            1847..3880
                     /gene="metG"
                     /locus_tag="ACHKBL_003443"
     CDS             1847..3880
                     /gene="metG"
                     /locus_tag="ACHKBL_003443"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708001.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HFO5820268.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYSRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRGDSV
                     SFDEYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLASELADPQLYKTFTDAAEEIGEAWESREFGKAVREIMALAD
                     LANRYVDEQAPWVVAKQEGRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLN
                     TELTWDGIQQPLLGHKVNPFKALYNRIDMKQVEALVEASKEEVKATTAPVTGPLADDP
                     IQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     ALIGRHTIMVANLAPRKMRFGISEGMVMAAGPGGKDIFLLSPDAGAKPGHQVK"
     gene            4022..7810
                     /locus_tag="ACHKBL_003444"
     CDS             4022..7810
                     /locus_tag="ACHKBL_003444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
                     /protein_id="HFO5820269.1"
                     /translation="MRHFIYQDEKSHKFWAVEQQGNELHINWGKVGTNGQSQVKSFAD
                     AAAAEKAGLKLIAEKVKKGYVEQAKDNSLQPSQTVTDSLKVADLSTIIQEQPSFVAET
                     RAADKNTDAVLPWLAKDIAVVFPPEVVHTTLSHRRFPGVPVQQADKLTQLRRLACSVS
                     QRDNKTATFDFSACSLEWQNTVAQAISQIDGLKTTQLPSPVMAVLTALEMKCTRYKER
                     EDVMDQIIQEGGLEYATDVIIHLQQISIEWDYVNNNIVFLSSGISPDYLQQYSSFELR
                     LRKHLSLAEESLWQKCAQKLIAAIPHIPEWRQPLIALLLPEKPEIAHEISQRLLGQKK
                     LPSLEWLKIVATDEHILASLEKYHEPYAIFDDYYCGAIWSATVLQEQGVTALPRFAPY
                     AASDYCADVLRHINHPFALTLLIRVAGHTKRCHDRMTKACAAFPHAALAALAELLVQK
                     EENSWRIMLMTMLISHPTLAEQVIPWLSTPAVAVLKSCQQQLTQPSNHASADLLPAIV
                     VSPPWLSKKKKSPIPVLDLAPLNLESICTITDTEAKEFQTHWDWEPHKPSEGAKNFLY
                     SLGYRRWDFDTYKYIGASDSAIDAWEREDFATLIQMFKAHHAPYQGEWHLNSLPFLPM
                     QKAIKLWEFLSKEPHTAIKPVMLYLRLAGMSGFLHSFSRYPQEGFAVANYFAATELAP
                     AVARAFNKLKTLRQDASSWLLKYPEHAITGLLPAALGKASEAQDNARAALRMLTENGH
                     QPLLQEIARRYNQPEVTDAVNALLALDALDNHPTKIPTLPTFYQPSLWTRPLLKANAQ
                     SLPDSALLHLGEMLRFPQEEALYPGLLQVKDACTTDSLAEFAWDLFTAWQTAGAPSKE
                     SWAFTALGVLGNDDTARKLTPLIRAWPGESQHKRATVGLDILAAIGSDIALMQLNGIA
                     QKLKFKALQERAKEKIADIAESRELTVAELEDRLAPDLGLDDNGSLLLDFGPRQFTVS
                     FDETLKPFVRDASGSRLKDLPKPNKSDDELQANNAVNRYKLLKKDARTVAAQQVARLE
                     SAMCLRRRWSPENFQLFLVEHPLVRHLTRRLIWGVYSAENQLLACFRVAEDNSYSTAD
                     DDLFTLPEGDISIGIPHVLEISPTDAAAFGQLFADYELLPPFRQLDRNSYALTEAERN
                     ASELTRWAGRKCPSGRVMGLANKGWMRGEPQDGGWIGWMIKPLGRWSLIMEIDEGFAV
                     GMSPAELSAEQLLSKLWLWEGKAESYGWGSNSTQEAQFSVLDAITASELINDIEALFE
                     "
     gene            7820..11452
                     /gene="yehI"
                     /locus_tag="ACHKBL_003445"
     CDS             7820..11452
                     /gene="yehI"
                     /locus_tag="ACHKBL_003445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein YehI"
                     /protein_id="HFO5820270.1"
                     /translation="MDKELPWLADNAQLELKYKKGKTPLSHRNWPGEPVPVITENIIQ
                     TLGDELLQKAEKKKNIVWRYENFSLEWQSAITQAINLIGEHKPSITARTMAALACIAQ
                     NDSQQLLDEIVQHEGLEYATEVVIARQFIVRCYESDPLVVTLQYQNEDYGYGYRSETY
                     NEFDLRLRKHLSLAEESCWQRCADKLIAALPGITKVRRPFIALNLPEKPEIANELVSL
                     ECSQTHFRSKEWLKVVADDPKAVKELARYWSQDIFSDREASYMSHENHFGYAACAALL
                     REQGLAAVPRLAMYAHKEDCGSLLVQINHPQVIRTLLLVADKNKPSLQRVAKYSKNFP
                     HATLAALAELLALKAPPARPGYPIIEDQKLPAQQKARDEYWHTLLQTLMASQPQLAEE
                     VMPWLSTQARAVLNSYLSAPPKTVIDSTDNSQMPEILVSPPWRSKKKMTAPRLDLAPL
                     ELTPQVYWQPGEQERLASTESARYFSTESLAERMEQKSGRVVLQELGFGDDVWLFLNY
                     ILPGKLDAARNSLIVQWHYYPGRVEEIMNGWSSPEAQLAEQALRSGHVEVLINIWEND
                     SYSRYRPEKSVWNLYLLAQLPREMALPFWLRINEKKHLSAGEDYFLSIFGLDALPGLL
                     LAFSHRPKETFPLILNFGATELALPVARVWRRFAAQRDLARQWILQWPEHTATALIPL
                     VFTKPSDNSEAALLALRLLYEQGHGELLQTAANRWQRTDVWPALEHLLKQSPIEIYPA
                     RMPKAPDFWHPLMWSRPRLIINHQLITDDALEIIGEMLRFTQGGRFHSGLEQLKTFCQ
                     PQTLAAFAWDLFTAWQQAGAPAKDNWAFLALSLFGDESTARDLTTQILAWPQEGKSTR
                     AVSGLNILTLMNNDMALIQLHHISQRAKSRPLRDNAAEFLQVVAENRGLSQEELADRL
                     VPTLGLDDPQALSFDFGPRQFTVRFDENLNPVIFDQQNVRQKSVPRLRTDDDQLKAPE
                     ALARLKGLKKDTTQVSKNLLPRLEAALRTTRRWSLADFHSLFVNHPFTRLVTQRLIWG
                     VYPANEPRRLLNAFRVAAEGEFCNAQDEPIDLPADALIGIAHPLEMTEGMRSEFAQLF
                     ADYEIMPPFRQLARRTVLLTPDESTSNSLTRWEGKSATVGQLMGMRYKGWESGYEDAF
                     VYGLGEYRLVLKFSPGFNHYNVDSKALMSFRSLRVYRDNKSVTFAELDVFDLSEALSA
                     PDVIFH"
     gene            11514..>11781
                     /gene="yehK"
                     /locus_tag="ACHKBL_003446"
     CDS             11514..>11781
                     /gene="yehK"
                     /locus_tag="ACHKBL_003446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YehK"
                     /protein_id="HFO5820271.1"
                     /translation="MIVQKELVAIYDYEVPVPEDPFSFRLEIHKCSELFTGSVYRLER
                     FRLRPTFHQRDREDADPLINDALIYIRDECIDERKLRGESPETVI"
BASE COUNT         3063 a         3014 c         2887 g         2817 t
ORIGIN      
        1 cttaaatcat tcatgtgaat gatttaagct ttctaaatcc gattaaaacc agggtggcaa
       61 attaatcgta gcgatttaac acgattcgac gtttgctatc gctatctgca gcaatcactt
      121 ccgtcgttac acctttaact ggcgaagaaa cctgatgacg ggtatcaaca ttatacgtta
      181 actgaccaca atgctgttga ggttgaccat taaccatagc catactgcat gtcgggggac
      241 tgatcctacc attaatcgtt aatgtggcag tttctgaaga gaacgcggga gaggccaatc
      301 cataggcaag aaaaataatt cctgacagcc aatacttatt cattgaacgt tatccctgta
      361 gtaaaggtta tgcctggata gaatgagtgc ataacaaact atagctgtac atccactaca
      421 cagccacgaa ggatgataat gaagcattgc ctgtacgatc aatcgacttt gtagagtttc
      481 ggacgaaggt ccgcagaata ttcgcagcat taaataagtg ttcattaatg cggcaaaaac
      541 aaatacaccg catcaatgta acatctctgc ggtgtatgaa gaataaccag aatggttaat
      601 acgcgttaga ccgcgcggaa ggaaatctcg cctggaatga cttcaccctg ccagtaaagc
      661 tgagctgcaa cgcggtcagc cagctggcgg tagatagcgg taaattcgct ctcaggacgg
      721 ctgataaccg ttggcgtgcc tttatcgaga tcctcacgta aggagatgtg taacggcatc
      781 tgccccagca gctgtgtgtg gtatttctcg gccagtttct ctgcaccacc ggtaccgaaa
      841 atcggttcat ggtgaccaca gttactgcaa atatgcacac tcatgttctc gacaataccg
      901 agcaccggaa cctcgacttt ctcgaacatc acaatgcctt ttttcgcatc gatcaacgcg
      961 atgtcctgcg gggtggtgac cacgactgca ccggttactg gaatgttttg tgccaatgtc
     1021 aattgaatgt caccggtacc tggcggcata tcaagcacca gatagtcgag atccggccac
     1081 aaggtttcct gcaacatttg cattaacgcc ttgctcgcca ttgggccacg ccacaccatc
     1141 gcattgtcgt ctgtcaccag ataaccaatt gaattagttg ccaggccatg agacataatc
     1201 ggtgccatat gggtaccgtc cggcgaagtt ggacgctgat tttctgcacc cagcatgtta
     1261 gggattgatg ggccgtagat atcggcgtcc agaatgccga ctttcgcccc ttccgccgcc
     1321 agtgccaacg ccaggtttac cgccgtggag gatttaccca cgccaccttt accggaactg
     1381 accgcgataa tgttcttcac gccgttaatt cccggctgat tcttcacgcg tttcagagtg
     1441 gctatgttat gcgacagttt ccagtcgata gctttcgctc cggtgatgcg cagcagttcg
     1501 gcgctacatt gctcttttaa ctcttcgaac gcgctatgcc agacgaatgg cataaccagt
     1561 tccacatgca gcgtgtcgtc catccaggcg acatggtgca acgctttcag cgttgtgagg
     1621 ttatgcttca gggttgggtg ctgaaaattg gccagtgtcc cggcgaccat tgcgcgcaag
     1681 gcttctggtg atttggcctg ggattgttcg ttcatcccga ctcctttttt gtatagataa
     1741 accatcagtt gatagtttac ctgaagaata tagagaagta cttacttaac attttcccat
     1801 ttggtactat ctaagccctt ttcactatta agaagtaatg cctactatga ctcaagtcgc
     1861 gaagaaaatt ctggtgacgt gcgcactgcc gtacgctaac ggctcaatcc acctcggcca
     1921 tatgctggag cacatccagg ctgatgtctg ggtccgttac cagcgaatgc gcggccacga
     1981 ggtcaacttc atctgcgccg acgatgccca cggtacacca atcatgttga aagctcagca
     2041 gcttggtatc actccggagc agatgattgg cgaaatgagt caggagcatc agactgattt
     2101 cgcaggcttt aacatcagct atgacaacta tcactcgacg cacagcgaag agaaccgcca
     2161 gttgtcagaa cttatctact ctcgcctgaa agaaaacggt tttattaaaa accgcaccat
     2221 ctctcagctg tacgatccgg aaaaaggcat gttcctgccg gaccgttttg taaaaggcac
     2281 ctgcccgaaa tgtaaatccc cggatcaata cggcgataac tgcgaagtct gcggcgcgac
     2341 ctacagcccg actgaactga tcgagccgaa atcggtggtt tctggcgcta ccccggtaat
     2401 gcgtgattct gaacacttct tctttgatct gccctctttc agcgaaatgc tgcaggcatg
     2461 gacccgcagc ggtgcgttgc aggagcaggt ggcaaataaa atgcaggagt ggtttgaatc
     2521 tggcctgcaa cagtgggata tctcccgcga cgccccttat ttcggttttg aaattccgaa
     2581 cgcgccgggc aaatatttct acgtctggct ggacgcaccg attggctaca tgggttcttt
     2641 caagaatctg tgtgacaagc gcggcgacag cgtaagcttc gatgaatact ggaagaaaga
     2701 ctccactgcc gagctgtacc acttcatcgg taaagatatt gtttacttcc acagcctgtt
     2761 ctggcctgcc atgttggaag gtagcaactt ccgcaagccg accaacctgt ttgttcacgg
     2821 ctatgtaacg gtgaacggcg cgaagatgtc caagtctcgc ggcaccttta ttaaagccag
     2881 cacctggctg aatcattttg acgctgacag cctgcgttac tactacactg cgaaactctc
     2941 ttcgcgcatt gatgatatcg atctcaacct cgaggatttc gttcagcgtg tgaatgctga
     3001 tatcgtcaac aaagtggtta atctggcgtc ccgtaacgcg ggctttatca acaaacgttt
     3061 tgacggcgtg ctggcaagcg aactggctga cccgcaactg tacaaaacct tcactgatgc
     3121 cgctgaagag attggcgaag cgtgggaaag ccgtgaattt ggtaaagcag tgcgcgaaat
     3181 catggcactg gctgacctgg ctaaccgcta tgtcgatgaa caggctccgt gggtggtggc
     3241 gaagcaggaa ggtcgcgatg ctgacctgca ggcgatttgc tctatgggca ttaacctgtt
     3301 ccgcgtactg atgacttacc tgaagccggt actgccgaaa ctgactgaac gagcagaagc
     3361 attcctgaat acggaactga cctgggatgg tatccagcaa ccgctgctgg gccataaagt
     3421 gaatccgttc aaagcactgt ataaccgtat cgacatgaag caggttgaag cactggtgga
     3481 agcgtctaaa gaagaagtga aagccactac cgcgccagtt actggcccac tggcagatga
     3541 tccgattcag gaaaccatca cctttgacga cttcgctaaa gttgacctgc gcgtggcgct
     3601 gattgaaaac gcagagtttg ttgaaggttc tgacaaactg ctgcgcctga cgctggatct
     3661 cggcggtgaa aaacgcaatg tcttctccgg cattcgttct gcttacccgg atccgcaggc
     3721 acttattggt cgtcacacca ttatggtggc taacctggca ccacgtaaaa tgcgcttcgg
     3781 tatctccgaa ggcatggtga tggctgctgg tcctggcggg aaagatatct tcctgctaag
     3841 cccggatgcc ggtgctaaac cgggtcatca ggtgaaataa tccccccttc aaggcgctgc
     3901 atcgacagcg ccttttcttt ataaattcct aaatttgttt tcttgcgatt ttgtctctct
     3961 ctaacccgca taaatactgg tagcatctgc attcaactgg ataaaattac agggatgcag
     4021 aatgagacac tttatctatc aggacgaaaa atcacataaa ttctgggcgg tggagcagca
     4081 gggaaacgaa ctgcacatca actggggtaa agtcggcacc aacggacaaa gccaggtaaa
     4141 aagttttgcg gatgcagcag cggcggaaaa agcgggactg aagctgattg cagagaaggt
     4201 gaagaagggg tatgtggagc aagcgaagga taattctttg caaccttccc aaacggtaac
     4261 ggactctctc aaggtagctg acttatccac cattattcag gaacaaccct ctttcgtagc
     4321 agaaacccgt gcggctgaca aaaatacaga tgctgtttta ccgtggctgg cgaaagatat
     4381 tgctgtcgtt tttccgcccg aagttgtaca caccacgtta agtcatcgcc gctttcccgg
     4441 agttcctgtt cagcaagcag acaaattgac ccaattacgt cgcttagcct gtagtgtgtc
     4501 gcaacgggat aataaaacag ccacatttga cttcagcgcc tgttctttag aatggcaaaa
     4561 caccgtcgcc caggcgatca gtcagatcga cggcctgaaa acaacacagc taccatcacc
     4621 cgtaatggct gtactcactg cacttgaaat gaaatgcaca agatataaag agcgtgagga
     4681 tgttatggat cagattattc aggaaggcgg tctggaatat gctactgatg taataataca
     4741 tcttcaacaa atatctattg aatgggatta tgtcaacaat aatattgttt tcctgtcgtc
     4801 tggcatttca cctgattact tgcagcaata ttccagcttt gaattacgcc tacgtaaaca
     4861 tttatcactg gcggaagagt ctctctggca aaaatgtgca caaaaactta ttgccgcaat
     4921 tccacatatt ccagaatggc ggcaaccatt aattgctttg ttattacccg aaaaaccaga
     4981 aattgcacat gaaatttccc agcgtctact ggggcaaaag aaattaccct cgcttgagtg
     5041 gctaaaaata gtggcgactg atgagcacat tcttgcctca ttagaaaaat atcacgagcc
     5101 atatgccatt tttgatgatt actattgtgg tgcgatatgg tcagccaccg tattacagga
     5161 gcaaggtgtt acagccctgc cccgatttgc tccctatgcc gcaagtgact actgcgccga
     5221 tgtgttgcgt catatcaatc atccgttcgc attaacactg cttatacgtg tagccgggca
     5281 tactaaacgc tgtcacgatc gaatgacgaa agcctgcgct gcgttcccac acgcagcact
     5341 ggcagcactg gcggaacttc tagtgcaaaa agaagagaat agttggcgca ttatgctaat
     5401 gacgatgctt atctcgcatc cgacactagc agaacaggtc attccctggc tttcgacacc
     5461 cgcagttgca gtgctgaaat catgtcagca acaactgaca cagccctcaa accatgccag
     5521 cgccgatcta ctgccggcca tagtagtctc cccaccatgg ctttcgaaaa agaaaaaatc
     5581 gccgattccg gtgctggatt tagcaccgct caacctcgaa tccatatgca caataaccga
     5641 cacagaagct aaagagtttc aaactcattg ggactgggaa ccgcataagc caagtgaagg
     5701 tgctaaaaat ttcctttact cgctggggta tcgccgctgg gattttgaca catataaata
     5761 tatcggcgct tctgactcgg caattgacgc atgggaacgt gaggacttcg ctacactcat
     5821 ccaaatgttt aaagcccacc atgcaccgta tcagggggag tggcatctca actcacttcc
     5881 ctttttacct atgcaaaaag cgattaagct atgggaattt ctcagcaaag agccgcatac
     5941 cgcgataaaa ccagtcatgc tttatttgcg actggcaggt atgagtggtt ttctacactc
     6001 gttttcacgc taccctcaag aaggttttgc ggttgctaat tattttgccg caacggaact
     6061 ggctcctgcc gtcgcccgcg ccttcaacaa actcaaaaca ttacgacaag acgccagtag
     6121 ctggctgctg aaatacccgg aacacgccat taccggcctg ctgcctgcag cgctcggcaa
     6181 agccagtgaa gcacaggaca acgcccgtgc tgccttgcgt atgcttaccg aaaacggtca
     6241 tcagccattg ctgcaagaaa tcgcccggcg ttataaccag ccggaagtaa ccgatgcggt
     6301 gaacgctcta cttgcgctcg atgccttaga taatcacccg acaaaaatcc ccactcttcc
     6361 gaccttttat cagccatcgc tctggacgcg cccgttatta aaagcaaacg cccaatcact
     6421 gcctgatagc gcactcctcc acctcggtga aatgctccgc ttccctcagg aagaggcact
     6481 gtatccggga ttattgcagg tgaaagatgc ctgtaccacc gactcactgg ctgaatttgc
     6541 ctgggatctg tttaccgcct ggcagaccgc tggcgcgccg tcgaaagaga gttgggcgtt
     6601 cactgcgtta ggcgttctcg gtaacgatga caccgcccgc aaactgacgc ctttgattcg
     6661 cgcctggcct ggtgaatccc agcataaacg cgccaccgtc gggttggata ttctcgctgc
     6721 tatcggtagt gatatcgccc tgatgcagct taacggcatc gcccagaaac tgaaattcaa
     6781 agcattacag gagcgggcaa aagaaaaaat tgccgacatt gccgagagcc gcgaactcac
     6841 ggtggcggag cttgaagatc ggttagcacc ggatctcggt ctggatgata atggttcgtt
     6901 gttgctggat ttcggcccac gccagttcac cgtcagcttt gatgaaacct taaaaccgtt
     6961 tgtgcgtgac gcttccggca gtcgtctgaa agacctgccc aaaccaaaca aaagcgatga
     7021 tgaattacag gcgaacaatg ctgttaaccg ctacaaattg ctgaaaaaag atgcgcgtac
     7081 cgtcgccgcc cagcaggtag caaggctgga atccgccatg tgcctgcgtc gccgctggtc
     7141 accggaaaac tttcagctct tcctggttga gcatccgctg gtccgccact taacccgccg
     7201 tctgatttgg ggcgtttata gcgccgaaaa ccagctactg gcttgctttc gcgtagcgga
     7261 agataacagc tacagcaccg ctgacgatga tcttttcacc ctgccggaag gcgatatctc
     7321 tattggtatt cctcacgttc tggaaatatc accgacggat gctgccgcct tcgggcagct
     7381 ttttgccgac tacgaactgt taccaccgtt ccgccagctt gatcgtaaca gttacgcgct
     7441 gacagaagcc gagcgcaatg ccagcgaact gacacgctgg gcaggcagaa aatgcccgag
     7501 tggtcgggta atggggctgg caaataaagg ctggatgcgt ggtgaaccgc aggacggtgg
     7561 ctggatcggc tggatgatca aacctctggg ccgctggtcg ttaatcatgg aaatcgatga
     7621 aggttttgcg gtcggcatgt cgccagccga actcagtgcc gagcaactct taagcaagct
     7681 gtggctatgg gaaggcaaag cggaaagcta tggctggggg agtaattcaa cacaggaagc
     7741 gcagttctcc gtactcgatg ccatcaccgc cagcgagcta attaacgata ttgaagccct
     7801 gtttgaataa ggaaagagca tggacaagga attaccgtgg ctggcggata acgcccaact
     7861 ggaactgaaa tataaaaaag gcaaaacacc actcagccat cgcaattggc caggcgaacc
     7921 ggtacctgtt atcactgaaa acatcattca gacactgggt gatgaattgc tacaaaaagc
     7981 agagaagaaa aaaaacattg tctggcgtta tgaaaacttt tcactggagt ggcagtccgc
     8041 catcacgcag gccatcaact tgatcggcga acacaaaccc tcaatcacgg cccggacaat
     8101 ggcggcgcta gcctgtatcg cgcaaaatga tagccaacag ttgctcgacg aaatcgtcca
     8161 acacgagggg ctggaatatg caactgaggt ggtgattgca cgccagttta ttgtacggtg
     8221 ttatgagagt gatcctctgg tagtaacatt gcaatatcag aacgaggact atggctatgg
     8281 ttatcgctca gaaacctata acgaattcga tctccgattg cgtaagcatc tctctctggc
     8341 agaggaaagc tgctggcagc gttgcgccga caaactcatt gccgcactgc caggaataac
     8401 caaagttcgc cgccctttta ttgcgctgaa cctcccggaa aaacccgaaa tcgccaacga
     8461 gctggtaagc cttgaatgtt cgcaaactca ttttcgctca aaagagtggt taaaagttgt
     8521 tgctgatgac cctaaggcgg tgaaagaact cgcacgttac tggagccagg atatatttag
     8581 cgatcgagaa gccagctaca tgtcgcatga aaaccacttc ggctacgcgg cctgcgctgc
     8641 ccttttgcgc gaacaaggac tggcagccgt tccgcgcctc gcgatgtatg cacataaaga
     8701 agattgcggc agtctgctgg tacaaattaa ccatccgcaa gtcatccgca ccttgctact
     8761 ggtggctgat aaaaacaaac ccagcctgca acgtgtggct aaatacagta aaaacttccc
     8821 ccatgcgacg ctcgccgcac tggcagaact gctggcgtta aaagcccccc ctgcgcgccc
     8881 tggttatccc atcatcgaag accaaaagct gcctgcacag caaaaagcac gtgatgaata
     8941 ctggcatacg ctgttacaaa cgctgatggc atcgcagcca caactggcag aagaggtgat
     9001 gccgtggtta agtactcaag cccgggcagt gctgaatagt tatttatcgg caccgcccaa
     9061 aacggttatt gatagcaccg ataacagcca aatgcctgaa atcctcgttt caccaccgtg
     9121 gcgtagtaag aaaaaaatga cagctccacg tcttgatttg gcaccgctcg aattaacccc
     9181 gcaagtttac tggcaaccag gcgaacaaga gaggcttgcc tccactgagt ctgcccgtta
     9241 tttcagcacg gaatctcttg ctgaacgcat ggaacaaaaa agtggacgag ttgtattaca
     9301 ggaactgggt tttggggatg atgtatggct gtttctgaat tatatactcc ccggaaaact
     9361 ggatgctgca cgcaattcac tcattgttca gtggcattac tacccagggc gggttgaaga
     9421 gatcatgaat ggctggagct ccccggaagc tcaattagca gaacaggcgc ttcgcagcgg
     9481 tcacgtagaa gtgttaatta acatatggga aaatgacagc tactcacgtt atcgtccgga
     9541 aaagagtgtc tggaacctgt atttattggc acagttgccg cgtgagatgg ctttgccttt
     9601 ctggctacgt atcaatgaga aaaagcatct gtctgcgggt gaggactatt ttctcagtat
     9661 cttcggattg gatgctctac caggtctgct gttggctttt tcacatcgtc caaaagaaac
     9721 atttccgtta attttaaatt ttggcgcaac agaactggcc ctgcccgttg cccgcgtctg
     9781 gcgacgtttc gcggcgcagc gtgatctggc ccgccagtgg attttacaat ggccggaaca
     9841 tacggctact gcacttatcc ctcttgtctt caccaaaccc agcgataata gcgaagctgc
     9901 attacttgcc ctacgtttac tttacgaaca gggacatggt gaattgctgc aaaccgcggc
     9961 aaaccgctgg cagcgtacag atgtatggcc tgccctggag catttactta aacagagtcc
    10021 aatagagatt tacccggcac gcatgccaaa agcccctgat ttctggcatc cgctaatgtg
    10081 gtccaggccg cgcctaatca ttaatcatca acttattacc gatgacgctc tggaaattat
    10141 cggcgaaatg ctgcgcttta cccagggggg acgttttcat agcgggctgg aacaactgaa
    10201 aacgttctgc cagccgcaaa cgctggcagc ttttgcctgg gatctcttca ctgcgtggca
    10261 acaagctggt gcccccgcaa aagacaactg ggcatttctg gcgttaagtc tctttggtga
    10321 cgaaagcacg gcacgggatc taacgacaca gatcctcgcc tggccacaag aaggcaaatc
    10381 tacccgtgct gtcagtggcc tgaacatcct taccctgatg aataatgata tggcgctgat
    10441 acagctgcat catatatcgc aacgggcgaa atcccgcccc ttacgtgata acgcggcgga
    10501 atttcttcag gtggtcgcag aaaatcgcgg gctaagccag gaagagttag cggacagatt
    10561 agtcccaaca ctgggccttg atgatccgca ggcgttgagt tttgattttg gtccccggca
    10621 gtttaccgtt cgcttcgatg aaaaccttaa cccggttatc tttgatcagc aaaacgttcg
    10681 ccagaaaagc gttccccggt tgcgcaccga tgacgatcaa ctgaaagcgc ccgaggcact
    10741 ggcccgatta aaagggctaa aaaaagatac gactcaggtg agcaaaaacc tgctcccccg
    10801 tcttgaagct gccctgcgta ccacccgacg ctggtcgctg gcagattttc attctctgtt
    10861 tgttaatcat ccctttaccc gtctggttac ccagcgatta atatggggcg tgtatccggc
    10921 aaatgaaccg cgtcgtttac tcaacgcctt tcgtgtggcc gcagaggggg agttctgcaa
    10981 tgcgcaagat gagcccattg acctgcctgc ggatgctctg attggcattg cccacccgtt
    11041 agaaatgaca gaaggaatgc gcagtgaatt tgcacagctt tttgccgatt acgaaattat
    11101 gccgcctttt cgccagttgg cgcggcgcac ggtgctgctc acacctgacg agtcaaccag
    11161 taacagcctg actcgctggg aaggtaaatc cgctaccgtt gggcagctta tgggaatgcg
    11221 atacaaaggc tgggagtcag gctatgagga cgcatttgtc tatggcctgg gcgagtaccg
    11281 gctggtcctt aagttttcac ccggttttaa ccactacaat gttgatagca aagcgttaat
    11341 gagcttccgt tctcttcgag tgtaccgtga caataaatcc gtcacttttg ccgaacttga
    11401 tgtgtttgat ttgagtgagg cgttaagcgc acctgacgtc attttccatt aaaaacacag
    11461 cgggcagtga tgcaactgcc cgttatcaac acgacttacc agcggggaaa gcgatgatcg
    11521 tgcaaaaaga gctggttgct atttacgatt atgaggtccc tgtacctgaa gatccgtttt
    11581 ccttcagact tgagatccat aaatgctctg aattatttac aggttccgtc tatcgactgg
    11641 agcgattccg gctacgtcca acatttcatc aacgtgatcg agaagatgct gacccgctaa
    11701 taaatgatgc gttgatttat ataagagatg agtgtattga tgagcggaaa ttacgaggtg
    11761 aatcacctga aactgtaata g
//
LOCUS       DAZFLD010000146        11748 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.147, whole genome shotgun sequence.
ACCESSION   DAZFLD010000146 DAZFLD010000000
VERSION     DAZFLD010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11748
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.147"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            69..1049
                     /locus_tag="ACHKBL_003447"
     CDS             69..1049
                     /locus_tag="ACHKBL_003447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid segregation protein ParM"
                     /protein_id="HFO5820272.1"
                     /translation="MKIFIDDGSTNIKLAWLEDGDVKTLISPNSFKPEWSFSLLDDAA
                     PANYEIDGEKFSFDPLSADAVVTTETRYQYSDVNVVAIQHALQQTGLKAQPVDVIVTL
                     PISEYLDANNQKNKQNIERKKKNVMREVRVQGSDAFVIRSVSVLPESIPAGFSVLAGL
                     EDDESLLIVDLGGTTLDVSHVRSKMTGITKTWCDPNIGVSLITSGVKEQMAVHANTRV
                     SSFQADNIIVHRNEPDYLSRRIYNAEQRESIINVINERQKLLIKRVNDVISRFTDYTH
                     VMCVGGGADIVAEAVKNLTKVPDERFYLSSSPQFDLVMGMIKMKGGVTNE"
     gene            1042..1458
                     /locus_tag="ACHKBL_003448"
     CDS             1042..1458
                     /locus_tag="ACHKBL_003448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="HFO5820273.1"
                     /translation="MSDENKSRRCSFELFPDERTGDKIADELIANEKLKERGRFMRAM
                     LVTGAAFAAIDKRLPLLISELLTENTTLDDINKVISSVIPGAFSVEKKLLELLEKQSG
                     LHTSVDCSTPLTEQSLSRNDGEDQTRRNAENMFGDD"
     gene            complement(1460..2734)
                     /locus_tag="ACHKBL_003449"
     CDS             complement(1460..2734)
                     /locus_tag="ACHKBL_003449"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001566943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006974 - DNA damage response [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0019985 - translesion synthesis [Evidence
                     IEA];
                     GO_process: GO:0042276 - error-prone translesion synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HFO5820274.1"
                     /translation="MFALADINSFYASCEKVFRPDLRNEPVIVLSNNDGCVIARSPEA
                     KALGIRMGQPWFQVRQMRLEKKIHVFSSNYALYHSMSQRVMAVLESLSPAIEPYSIDE
                     MFIDLRGINHCISPEFFGHQLREQVKSWTGLTMGVGIAPTKTLAKSAQWATKQWPQFS
                     GVVALTAENRNRTLKLLGLQPVGEVWGVGRRLTEKLNALGINTALQLAQANTAFIRKN
                     FSVILERTVRELNGESCISLEEAPPAKQQIVCSRSFGERITDKDAMHQAVVQYAERAA
                     EKLRGERQYCRQVTTFVRTSPFAVKEPCYSNAAVEKLPLPTQDSRDIIAAACRALNHV
                     WREGYRYMKAGVMLADFTPSGIAQPGLFDEIQPRKNSEKLMKTLDELNQSGKGKVWFA
                     GRGTAPEWQMKREMLSQCYTTKWRDIPLARLG"
     gene            complement(2734..3171)
                     /gene="umuD"
                     /locus_tag="ACHKBL_003450"
     CDS             complement(2734..3171)
                     /gene="umuD"
                     /locus_tag="ACHKBL_003450"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HFO5820275.1"
                     /translation="MSTVYHRPAAPSGDDSYVRPLFADRCQAGFPSPATDYAEQELDL
                     NSYCISRPAATFFLRASGESMNQAGVQNGDLLVVDRAEKPQHGDIVIAEIDGEFTVKR
                     LLLHPRPALEPVSDSPEFRTLYPENICIFGVVTHVIHRTRELR"
     gene            complement(3168..3416)
                     /locus_tag="ACHKBL_003451"
     CDS             complement(3168..3416)
                     /locus_tag="ACHKBL_003451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HFO5820276.1"
                     /translation="MIRIEILFDRQSTKNLKSGTLQALQNEIEQRLKPHYPEIWLRID
                     QGSAPSLSVTGARNDKDKERILSLLEEIWQDDSWLPAA"
     gene            3834..4736
                     /locus_tag="ACHKBL_003452"
     CDS             3834..4736
                     /locus_tag="ACHKBL_003452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
                     /protein_id="HFO5820277.1"
                     /translation="MYFSAEPAQITEIKRLASGAVTPLYRRATNEGIQLFLAGSAGLL
                     QTTEDVQFEPCPGLTAAGRGVVSPENIAFTRWLTHLQDGVLLDEQNCLMLHELWLQSG
                     TGQRRWEGLPDDVRDTITALFTAKRGDWCGFWSNEDVSVWWNRLCDNVLPEKTMPFDL
                     LTVLPTRLDVEVNGFNGGVLNGVPSAYHWYTEQYGVKWPCGYEVNISSQGDNFIKVDF
                     DTPWCQPESDVIAELSRRFSCTLEHWYAEQGCDFCGWQLYERGELVDVLWGELEWSSP
                     TDDDELPEVTGPAWIVDKVAHYGG"
     gene            4733..5044
                     /locus_tag="ACHKBL_003453"
     CDS             4733..5044
                     /locus_tag="ACHKBL_003453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5820278.1"
                     /translation="MKYDGDGRATARFFSGKGCRRAPLFTAPADAARHKRCLWSVSRV
                     RRARDGRFYRSRLASVTVYASPSPFSDERPSSRFRGITLPSKRRRLRYSTVGLTRYRT
                     R"
     gene            5120..5191
                     /locus_tag="ACHKBL_003454"
                     /pseudo
     CDS             5120..5191
                     /locus_tag="ACHKBL_003454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858331.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5273..6973)
                     /gene="ltrA"
                     /locus_tag="ACHKBL_003455"
     CDS             complement(5273..6973)
                     /gene="ltrA"
                     /locus_tag="ACHKBL_003455"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFO5820279.1"
                     /translation="MTAKNINAGAPTDIVTHWHNINWAKCRREVRRLQARIVKAIQEE
                     KAGKVKALQWLLTHSFSARAMAVKRVTDNRGKRTPGVDGKTWSKPGSKMKAIYTLKRR
                     GYKPLPLRRIYIPKSNGKKRPLGIPTMKDRAMQALYLMALEPVAETTADPNSFGFRPC
                     RSTADAIEQCFTTLHRADRAQWILEADIRSCFDEISHEWLIANIPTDTAILKRWLKAG
                     YIDLGKLYPTSAGTPQGGIISPTLANMVLDGLQPLLKKTFYRGGLNPEKINIIRYADD
                     FVITGISHDTLSEKVLPLLENFLAERGLTLSPEKTRITHISDGFDFLGMNIRKYNGKL
                     LIKPSPQKVKEFLKRVRQTIKDNPTVSQSTLIWHLNPVLKGWVRYYRHVVSKKIFQKV
                     SHEIWKALWRWACRRHPKKGTGWIRKKYFRSVNFRHWVFGTETGKLLPSGKRELLALY
                     EISDMPVKRHRQIKSTANPFDPSYESYFEARLKATMLESIQGYSRLRNLWLRQGGQCP
                     VCHLPVNATTGWQLRHIVPKSEGGLDNIGNLTMLHPACHHTAKHQGISVMKPALMWSY
                     "
     gene            7595..8206
                     /locus_tag="ACHKBL_003456"
                     /pseudo
     CDS             7595..8206
                     /locus_tag="ACHKBL_003456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052645.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
     gene            8207..8428
                     /locus_tag="ACHKBL_003457"
     CDS             8207..8428
                     /locus_tag="ACHKBL_003457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5820280.1"
                     /translation="MNYAGHEKLRAEVAEVANAMCDLRTTMNEMERRYSFNADTLPER
                     LVRQTLFRANRLLMEAYTEILELDSCFKD"
     gene            8388..8874
                     /locus_tag="ACHKBL_003458"
                     /pseudo
     CDS             8388..8874
                     /locus_tag="ACHKBL_003458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052646.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
     gene            8919..9689
                     /locus_tag="ACHKBL_003459"
     CDS             8919..9689
                     /locus_tag="ACHKBL_003459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5820281.1"
                     /translation="MNKTLNALVCRHARNLLLAQGWPEETDIEQLNSHYPGWISIYVR
                     LDVSRLATLLISRHDGVLPPVLASAMQKLTGTVAELVLSGSQWQALPVLPADGTQVSF
                     PYAGEWLTEEEIRAVLDAVRNAVRSICYQVADDARRIRAALTTTGQTLLTRQTRRFRL
                     VVKESDYPCWLDEDDENLPVVLDAILNRGARFSSVEMYLVCECVEHILASGLVCDVLR
                     IPDEPSRRWFDRDILREVVLEARDEIRSMADALAKIRK"
     gene            10103..10528
                     /locus_tag="ACHKBL_003460"
     CDS             10103..10528
                     /locus_tag="ACHKBL_003460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFO5820282.1"
                     /translation="MQYAKPVTLNVEECDRLSFLPYLFGNDFLYAEAYVYALAQKMMP
                     EYQGGFWHFIRLPDGGGYMMPDGDRFHLVNGENWFDRTVSADAAGIILTSLVINRQLW
                     LYHDSGDAGLTHLYRMRDAQLWSHIEFHPECNAIYAALD"
     gene            10575..10994
                     /locus_tag="ACHKBL_003461"
     CDS             10575..10994
                     /locus_tag="ACHKBL_003461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000271690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
                     /protein_id="HFO5820283.1"
                     /translation="MYCTVKEIIREVLNTDVPDSECVFAVVLTRGDVRHIARDWNLSD
                     DELETVMQRLDDAFVYGACDRVVSDIVNELMEEKRVNRLVTVPAVLLEKVMVMAGSEI
                     YRLHAVGSENGGDGDAFVREEREIMRVMRQALDGENG"
     gene            10994..11185
                     /locus_tag="ACHKBL_003462"
     CDS             10994..11185
                     /locus_tag="ACHKBL_003462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5820284.1"
                     /translation="MNISTETREILRNYKAVINARRREMGQKPLTTAQIVDEICDFVA
                     NQQAVFLGGHYILQGSRNR"
     gene            11199..11500
                     /locus_tag="ACHKBL_003463"
                     /pseudo
     CDS             11199..11500
                     /locus_tag="ACHKBL_003463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000386902.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(11500..11595)
                     /locus_tag="ACHKBL_003464"
                     /pseudo
     CDS             complement(11500..11595)
                     /locus_tag="ACHKBL_003464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052655.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         2525 a         3036 c         3226 g         2961 t
ORIGIN      
        1 tgaatataca ttgcatgtat gaagcatgtt tgttttttct atttgtttat gagcaacgga
       61 gactggttat gaaaatcttt attgatgatg gttctaccaa cattaaactt gcctggttgg
      121 aggatggtga cgtgaaaacg ttaatcagcc ctaacagctt taaaccggaa tggtctttta
      181 gtttactgga tgatgctgct ccggctaatt acgagatcga cggcgagaag ttttcatttg
      241 atccattaag tgctgatgct gtcgtaacga ctgaaacacg ctatcagtac agtgatgtga
      301 atgttgtggc cattcagcat gcgctacagc agaccggcct gaaagcgcag cctgttgatg
      361 tcatcgtcac attgccgatc tctgaatacc ttgacgctaa caatcagaaa aataagcaaa
      421 acattgaaag aaagaaaaaa aatgtgatgc gtgaagtacg ggtgcagggg agtgatgcat
      481 ttgttattcg cagtgtttcc gttcttcctg aaagtattcc cgctggtttc agtgttcttg
      541 cggggcttga ggatgatgaa tcccttttga tcgtagatct cggtggtaca actcttgatg
      601 tgtcgcatgt tcgtagcaaa atgacaggga ttacaaaaac ctggtgtgat ccgaatatcg
      661 gcgtgtccct gattacatcc ggggttaagg aacagatggc tgtacatgct aatacaagag
      721 tgagctcatt ccaggctgac aatatcattg tgcaccgtaa tgaacctgac tacctttccc
      781 gtcgcattta taatgctgaa cagcgtgagt ctatcattaa tgttatcaat gaacggcaga
      841 agttgcttat taaacgagtt aatgatgtca tcagccgttt caccgactat acacatgtaa
      901 tgtgtgttgg tggtggggct gacattgtgg ccgaggctgt gaagaacctg actaaggttc
      961 ctgatgagcg cttttatctg agctcatccc cgcagtttga tttagtcatg ggaatgataa
     1021 aaatgaaagg tggtgtgact aatgagtgat gaaaataaat ccagacgctg ctcatttgaa
     1081 cttttcccgg atgaacggac aggagataag atagcagatg agctcattgc aaatgaaaaa
     1141 ctgaaggagc ggggacgttt catgcgcgcg atgttagtta ctggtgcggc gtttgcagct
     1201 atagataaac gtcttccttt gctgatatcg gagttactga cagaaaatac gactctggat
     1261 gatattaata aggtgatttc cagcgtaata ccaggtgctt tttcggttga aaagaaattg
     1321 cttgaattac tggaaaaaca atctggtctc catacgtctg tggattgcag tactcctctt
     1381 acagagcaga gtctgtcgag aaatgacggt gaagaccaga ccagaagaaa cgctgaaaat
     1441 atgttcggag atgactgaac taacccagcc tggcaagggg aatatctcgc catttagttg
     1501 tataacactg actcaacatt tcccgtttca tttgccattc aggggcggtt cctcgccccg
     1561 caaaccacac tttccctttt cccgactggt tcagttcatc gagtgttttc attaactttt
     1621 cactgttttt acggggctgg atttcatcaa ataatcccgg ctgcgctata cccgatggtg
     1681 tgaaatcagc cagcatgact cctgccttca tatagcggta cccttcacgc cagacatggt
     1741 ttaaggctct gcatgcggcg gcaataatgt cccggctgtc ctgtgtgggc aatggaagct
     1801 tttccacagc ggcattgctg taacagggtt cttttactgc aaagggggat gtccgtacaa
     1861 atgtcgtcac ctgccggcaa tactgacgct ccccacgtag tttctctgcg gcccgctctg
     1921 catactgaac aacagcctgg tgcatggcat ctttgtctgt gattcgttca ccaaaactgc
     1981 gactacagac aatctgctgt tttgccggtg gtgcttcttc cagggatatg caggactcgc
     2041 cgttgagttc gcgtaccgta cgctcaagaa tgacgctgaa gtttttccgg atgaatgccg
     2101 tgttagcctg cgccagctgc agtgctgtgt taatacccag cgcattcagc ttttccgtca
     2161 gtctgcgtcc tactccccag acctcaccaa ctggctgcag ccccagtagc ttcaaggtcc
     2221 gattacggtt ttctgccgtc agcgcgacca ctccggaaaa ctgtggccat tgctttgttg
     2281 cccactgtgc acttttagcc agcgtttttg taggcgcaat gcccaccccc atggtgagtc
     2341 ctgtccagct ctttacctgt tccctgagct gatgaccaaa aaactccgga gagatgcaat
     2401 gatttatccc ccgcaaatca ataaacattt catcaattga gtagggctca attgcgggag
     2461 aaagcgactc cagaacagcc atgacccgtt ggctcatgct gtggtacagc gcataattgc
     2521 tggaaaatac atgtattttc ttctccaggc gcatctgtct cacctgaaac cagggctgcc
     2581 ccattctgat gccaagggct tttgcctccg ggctgcgcgc gatcacacag ccatcgttat
     2641 tgctgagtac gatgaccggt tcgttgcgaa ggtccgggcg gaaaactttt tcacatgagg
     2701 cgtagaaact gttgatatca gccagtgcaa acatcagcgt aactcccgcg tcctgtgtat
     2761 cacgtgagtg acaacaccaa aaatacagat gttttccgga tacagtgtgc ggaactccgg
     2821 gctgtctgaa accggctcca gtgccgggcg tgggtgcaac agcagtcgtt tgacggtgaa
     2881 ctccccgtcg atctcagcga taacgatgtc cccgtgttgt ggtttttcgg ccctgtccac
     2941 taccagcaga tcaccattct gcacgccagc ctggttcatc gattcaccgc tggcgcgcag
     3001 aaagaaggtg gctgcaggtc tgctgatgca atagctgttc agatccagtt cctgctcagc
     3061 ataatcagtg gcaggagaag gaaaaccggc ctggcaacga tcggcaaaca acggtcgcac
     3121 gtaactgtca tcgcctgaag gggcagccgg acggtgataa acggtactca tgctgcaggc
     3181 agccagctgt cgtcctgcca gatttcttcc agcagagaca ggatacgttc tttatccttg
     3241 tcgttgcggg ctcctgtgac agatagagac ggggcgctgc cctggtctat acgcagccag
     3301 atttccgggt agtgtggttt cagacgttgt tcgatttcat tctgtagcgc ctgaagtgtg
     3361 cctgatttga ggttttttgt gctctggcgg tcgaaaagaa tttcaatgcg gatcatgttc
     3421 tcatctccct tttcttttgc tgtatatgta tacagtattc taataactga atccgcacac
     3481 agtcaaatgt cattgttctg actaaaaagc gatcaattct ctgagggggt ggctgtatgc
     3541 ctgctgtgga gtgctgtatc cagcttatcc acagcatttt gtgcacggtt ctgtggacaa
     3601 aatacctggt tacccaggcc gtgccggcac gttaaccggg ctgcatccga tgcaagtgtg
     3661 tcgctgtcgc cggcctcctc acccggtcac gtttcgtcgt tcctcctcca cgcgctgcgg
     3721 cttcggggcc gcacctgcat tcgtatgcgg tcgcccggtt acaggtgcgg cacggcctga
     3781 tggaggccgc atgtgagagg agaattccta tgccaaactg gtgctcgaat cgtatgtatt
     3841 tttctgccga accggcacag attactgaga ttaaacgact ggccagcggt gcagtcacac
     3901 cgctttatcg ccgcgccaca aatgaaggta ttcagctgtt tctggccgga agtgccggac
     3961 ttctgcagac cactgaagat gtgcagtttg aaccgtgccc cggactgacg gctgccggac
     4021 gtggtgttgt atcgccggag aatatcgcgt tcacccgctg gctgacacac ctgcaggacg
     4081 gtgtactgct ggatgagcaa aactgcctga tgctgcatga actctggctg cagagcggta
     4141 ctggacagcg tcgctgggaa ggattaccgg acgatgtcag ggataccatc accgcacttt
     4201 tcaccgcaaa aagaggtgac tggtgtggct tctggagtaa cgaggatgta tcggtgtggt
     4261 ggaaccgtct gtgtgacaac gtactgccgg aaaaaaccat gccgtttgac ctgctgacgg
     4321 ttctgccgac ccgcctggat gttgaagtga atggctttaa cggtggtgtt ctgaacggtg
     4381 ttccttctgc atatcactgg tatacggaac agtatggcgt gaagtggcct tgtgggtatg
     4441 aggtgaatat cagcagtcag ggagacaact tcattaaggt ggatttcgac acgccgtggt
     4501 gtcagccgga aagcgacgtt attgcagaat taagccgccg tttcagctgc acgctggagc
     4561 actggtatgc cgaacagggc tgtgatttct gtggctggca gttgtatgag cgcggagagc
     4621 tcgttgatgt gctgtggggg gaactggaat ggtcttcccc gacagatgac gatgagctgc
     4681 cggaagtcac cggacctgcg tggatagtcg acaaggtggc gcattatggc ggatgaagta
     4741 tgacggagac gggcgggcaa ccgcccgttt cttttccggt aaaggatgtc gccgtgctcc
     4801 tctttttact gcgccggctg acgcggcgcg gcataaacgc tgcctgtggt cagtgtcccg
     4861 cgtccggcgg gcacgggacg ggcgctttta ccgctcccgg ctggcgtcgg tgacagtgta
     4921 cgccagcccg tcgccctttt ctgatgaacg cccttcaagc cgctttcgcg gcataacctt
     4981 gccgtcaaaa agacgacggc tgcggtattc cacggtcggc ctgacccgtt accggacgcg
     5041 gtgaacagcc cacaggcagc ggggaacggg caccacaggg gtgccctccc ggagccctct
     5101 gtaagagaag gagttttgta tgtcccgttt tgtccttggt aactgcattg atgttatggc
     5161 ccgtattcct gataacgcca ttgatttcat gtcgggtagc agctgcacat tactgtgcag
     5221 ccgcccccta agaaccgtac gtgccagttt cccggcatac ggctcaagcc tttcaataac
     5281 tccacattag agccggcttc ataacagata ttccctgatg ttttgcagtg tgatgacatg
     5341 ccggatgtaa catggtcaga ttacctatgt tatccagtcc cccttcggat ttcggcacaa
     5401 tgtgtcgaag ttgccagcct gttgtggcgt tcactggcag gtggcagacg gggcactgac
     5461 ctccctgtcg gagccacaga tttcttaggc gagagtaacc ctgtatggat tccagcatcg
     5521 tagcttttag ccgtgcttca aagtacgatt cgtagctggg atcaaaaggg ttagcagtgc
     5581 tttttatttg tctgtgtctt ttgactggca tatccgagat ttcatatagc gccagtaact
     5641 cacgtttgcc tgatggcagg agcttccctg tttctgtgcc gaatacccag tgtcggaagt
     5701 taactgaacg aaagtatttc tttcttatcc agcctgtacc tttctttggg tgtcgtcgac
     5761 atgcccagcg ccatagtgct ttccatattt catgactgac tttctgaaag atcttttttg
     5821 aaacaacatg acgatagtat ctgacccagc ctttgaggac tgggttgaga tgccatatca
     5881 gtgtgctctg gctgactgtc ggattgtctt ttatcgtctg gcggaccctt ttcaggaatt
     5941 cctttacctt ttggggtgaa ggtttaatca acagtttccc gttatatttg cggatgttca
     6001 tacccagaaa gtcgaatcca tcactgatgt gtgtgattcg tgtcttttct ggtgagaggg
     6061 taagccctct ttctgccaga aagttttcta acaagggaag aaccttttct gacagcgtgt
     6121 cgtggcttat ccccgtgatc acaaaatcgt ccgcataacg gattatgttg atcttttctg
     6181 gattcagtcc gcctcgatag aaggttttct tcaacagagg ctgcagacca tccagaacca
     6241 tgtttgccag cgttggagat attattccgc cctgtggagt tcctgcactg gttgggtata
     6301 atttccccaa atcaatatat ccggctttca gccatcgttt caggatcgcc gtatccgttg
     6361 ggatattggc gattagccat tcatgtgaaa tctcatcaaa acaactgcga atgtcggctt
     6421 ccagaatcca ttgtgcccga tcagccctgt gcaacgttgt gaaacattgc tcaatggcat
     6481 cggctgttga gcgacagggc ctgaacccaa acgaatttgg gtcagccgtt gtttctgcaa
     6541 caggttccag cgccatcaga tagagtgcct gcatggctct gtctttcatt gtcgggatcc
     6601 ctaatggtcg ctttttgccg ttagattttg ggatatatat acgccgtagc ggaagaggtt
     6661 tgtaacccct tcttttcagc gtgtatatag ccttcatctt gctgccgggt tttgaccatg
     6721 ttttgccatc aacacccggc gttctcttcc ctctgttatc tgtcactcgt ttaactgcca
     6781 ttgctctggc gctgaacgag tgggtgagta gccattgcaa ggcttttacc ttgcccgctt
     6841 tctcctcctg tattgccttc acgatacgcg cctgcagcct tctgacctcg cgacggcact
     6901 tggcccagtt gatattgtgc cagtgagtca cgatgtcggt gggtgcaccc gcgtttatgt
     6961 ttttcgcagt catctgctta ccctccttca aaggtattca ggttatctcg tgttgaaaga
     7021 ccatgcggaa gtctgcccgc tttcgcgtgg aatgatgttg ctgaaacagc tcaactcctg
     7081 tccatcccat tacaggatgg cattcgcttt ctccgcactc ctgtacccgc atcactgaca
     7141 gctttcctcg cggttagcct gccctgaggg cagtgatacg ggcttaccgt gttccgcaca
     7201 gtttgcaggg atgggttagg ttctgcctat ccaccggtag ctttgatgtc cgtgcctccc
     7261 cagtcaaaag aggaaggtcc agctacgttc ccattttggg tcgggcctgt cagtgccttt
     7321 ggcccgttca gggttgacga tgtttatcag cagttcacat gtgttaaccc tgccatccag
     7381 ccttgcgccc catccgccct gacactggca gacatgcttc ctcctcgcgg attccacatg
     7441 gtgtgaaaac cgggctacgt tgtcccgaag gcttcatacc gaacagttac ccgctctgca
     7501 tgcttcggta ggctacaggc gacggaacgc ctggttttac tacttccttt acatgaagtt
     7561 aaaacaacaa actgtgcgac tttcacgtcg cacccttacc gatcctccat atctcgtcgg
     7621 tttccgtgac cgtcaggggc gcaccattgc cggtgacgtt aatgatgact ggctgcaacc
     7681 tgccagcaat gaaatgtacc gcgtactcaa aaaagatgcg ctgatggtga gtttctatgg
     7741 ctggaaccgt attgaccgct ttatggcagc ctggaaacgc gccggattca gcgtggcagg
     7801 gcaccttgtg ttcaccaaaa actacacatc caaatctgcg tatgtcgcgt accgccacga
     7861 atgcgcttac atcctggcaa agggcagtcc ggcccggccc cgtaacccgc tgccggatgt
     7921 gctgggctgg aaatattcgg gcaaccgcca tcacccgacg gaaaagcccg ttaccagcct
     7981 gcaaccactg attgagagct tcacacatcc gaacgcgatt gtgctggacc cgtttgcagg
     8041 cagcggctca acctgcgtcg ccgccctcca gtccggacgc aggtatatcg gtatcgagct
     8101 gcttgagcag tatcaccgtg ccggacagca acgtctggct gccgtacagc gggccatgca
     8161 gcagggggcc gcaaatgatg actggtttat gccggaggct gcgtaaatga actatgccgg
     8221 acatgaaaaa cttcgcgccg aagtggcgga ggtggccaat gccatgtgcg acctgcgtac
     8281 aaccatgaat gagatggagc ggcggtacag ctttaatgct gacaccctgc cggaacgtct
     8341 ggtgcgtcag acgttgtttc gcgcaaaccg cctcctgatg gaggcatata ccgaaattct
     8401 ggaactggat tcctgcttca aagattgagg ggagagagta tgtacggaac atgcgaaata
     8461 ctctgccggg agctggcagc aaagtatccg ggagacacgc cgctgatgct ggttatctgg
     8521 tctccggaag agattcaggc ccttgctgac ggaatggata tttccctgtc cgatcatgaa
     8581 atcaggaccg tcctggcgcg cctggaggac atcccggaag accagcggac tgagtccggt
     8641 atttcttcgg gtgcggctat ggagattatc agcaacgtga gtgaaaaccg ccaggtgacc
     8701 gtccctgctg aactgctggc gtccctgatt cagaccgctg aacaggcatt gtggaagcgt
     8761 gaatgggccg cccgtgataa cggcctcgtc gtcccggaat gcgtcacccg ccgtcaggcg
     8821 gtggttaatc aggcccgcgc cctgctgaaa aacaacacac acgaaaacga ctgatgttat
     8881 cgccgctttc gggcggcgat tcagggagac cattaatcat gaacaaaacg ctgaatgcac
     8941 tggtttgtcg ccacgcccgt aacctgctgc tggcgcaggg ctggccggaa gagacggata
     9001 ttgaacaact gaattcgcac tatccgggct ggatcagcat ttatgtgcga ctggatgtat
     9061 cccggctggc gacgttactt atcagccgcc atgatggcgt gctgccgccg gtcctggcgt
     9121 ctgccatgca aaaactgacc ggaaccgtgg cggaacttgt actgtccggc agccagtggc
     9181 aggcgctgcc ggtacttccg gcagacggaa cgcaggtgtc tttcccgtat gccggtgaat
     9241 ggctgacgga ggaagaaatc cgggcagttc ttgatgcggt gcgcaatgca gtacgaagca
     9301 tctgttacca ggtggcagac gatgcgcggc gtatccgggc ggcgctgacc accaccggtc
     9361 agacgttgct gacccgccag acgcgccgct ttcgtctggt tgtgaaggaa agcgattacc
     9421 cctgctggct tgatgaagat gacgaaaatc tgcccgtggt gctcgatgcc attctgaacc
     9481 ggggcgcccg tttttcctct gtggaaatgt acctggtctg tgagtgtgtg gagcatatcc
     9541 tggccagtgg tctggtctgt gatgtgctgc gtataccgga tgaaccgtcc cgtcgctggt
     9601 ttgacaggga tattctgcgg gaggttgttc tggaggcacg ggatgaaatc cgcagcatgg
     9661 cagatgcact ggcgaaaatc cggaaatgat gacgctggcg gggaaaaatc tccgcctttt
     9721 cctgccggtc cccggacaga gcaaaaggat gtcgccgcag gctccgcttt acccggccat
     9781 gcggggcatg gccttgtggg ttttcagtta tgtggcctca gcgtcgtgtg cgggctgtgc
     9841 cgtgcctcca tcttagccgg gctggcgggg atgcaagggt aaaatgcacg cggcggtcac
     9901 ccggtccctc cttcccgtct gccgtgattt tccggtcgtt cacccgctca gatttcgcgg
     9961 tctcaccctc caactcccgt cagccgtgtg cgtcaggctg cggcttccct tgcgcccttg
    10021 catccccgcc ttatcgcccg gcttttctgg aggcacggca ccgcccggtg tcgcggaata
    10081 tgtgaacaac ggaggaaatc ccatgcagta tgcgaaacct gtcactctga acgttgaaga
    10141 gtgcgaccgc ctttcctttc tgccttacct gtttggcaac gattttctgt atgccgaggc
    10201 gtatgtgtac gcgctggcgc aaaaaatgat gccggaatat cagggaggct tctggcattt
    10261 catccgcctg ccggatggtg gcggttacat gatgccggat ggcgaccgtt ttcatctggt
    10321 caacggtgaa aactggtttg accgcactgt gagtgctgat gccgcaggta tcatcctgac
    10381 ttcccttgtg attaaccgcc agttgtggct gtaccacgac agcggggatg cgggactgac
    10441 acacctttac cggatgcgtg atgcgcagtt gtggagccac attgaatttc atcccgaatg
    10501 taacgcgatt tacgcagcac tggactgatt aacggacggg gcggcgatgc cgcccctgta
    10561 aaggagacga gaacatgtac tgtactgtta aagaaattat ccgcgaagtg ctgaatacag
    10621 acgtgccgga cagtgaatgt gtttttgccg tggtgctgac ccgcggggat gtgcgccaca
    10681 tagcccggga ctggaacctg tcagacgatg agctggaaac cgtcatgcag cggctggacg
    10741 atgcttttgt atatggcgca tgtgacagag ttgtttccga tattgtcaat gaactgatgg
    10801 aggaaaagcg cgtaaaccgt cttgtaacag tccctgcggt gctgctggaa aaagtgatgg
    10861 tgatggctgg tagtgaaatt tatcgcctcc atgctgtagg gagcgagaac gggggcgacg
    10921 gtgacgcatt tgtcagggaa gaacgtgaga ttatgcgggt aatgcgtcag gcgctggacg
    10981 gggagaacgg gtaatgaata tcagcacgga aacccgcgaa attctgcgta attacaaagc
    11041 tgtgattaat gcgcggcgtc gtgaaatggg gcagaaaccg ctcaccaccg cgcagattgt
    11101 tgatgaaatc tgtgattttg tggcgaatca gcaggcggtt ttcctcggtg gtcactatat
    11161 ccttcagggc agcagaaaca ggtgatacag ggcaggcggt ggaggccgcc tgtcagcccg
    11221 cgcgaaaacc cctggcaggc tggcgcctgc cggaagggaa gcgggccacg ctggcgcgtc
    11281 gcccgttacc cttcctgttg gtgacaggcg ttgtttcagg aagtacctgt tacagacctg
    11341 tcgttaaaga accttcttac acatgcgccc cttcggctcc cggctgcctg gcggcatccg
    11401 gtcgtcggcc cgactacgac aggcagctgc tggcgcaccc gccagtctcc gccgaacccg
    11461 ctggcgcggt ttcgggctgt cgctacggtc cgatgcgtga aaacccgggc ttgcagcgac
    11521 cggaacggtc gcgccgcctt tgtcgcgccg tggcctgcgc tcctggcatc ccggcaaaag
    11581 gccgccggga tgtaaggaaa ccgcgcctga tggcgcggca gtctcctctt ctgccgccac
    11641 ctctgcggcc tgtttcccgc tgtggacttt ccggggggca gacgtaacca cctcccgttc
    11701 catcagtcca ggggaagata taccggccac aaaattttta gccatgaa
//
LOCUS       DAZFLD010000147        11653 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.148, whole genome shotgun sequence.
ACCESSION   DAZFLD010000147 DAZFLD010000000
VERSION     DAZFLD010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11653
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.148"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..719
                     /locus_tag="ACHKBL_003465"
     CDS             <1..719
                     /locus_tag="ACHKBL_003465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0030674 - protein-macromolecule adaptor
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MipA/OmpV family protein"
                     /protein_id="HFO5820285.1"
                     /translation="ALFALPFMASATASELSIGAGAAYNESPYRGYNENTKAIPLISY
                     EGDTFYVRQTTLGFILSQSEKNELSLTASWMPLEFDPTDNDDYAMQQLDKRDSTAMAG
                     VAWYHHERWGTVKASAAADVLDNSNGWVGELSVFHKMQIGRLSLTPALGVLYYDENFS
                     DYYYGISESESRRSGLASYSAQDAWVPYVSLTAKYPIGEHVVLMASAGYSELPEEITD
                     SPMIDRNESFTFVTGVSWRF"
     gene            complement(840..2819)
                     /locus_tag="ACHKBL_003466"
     CDS             complement(840..2819)
                     /locus_tag="ACHKBL_003466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003581012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HFO5820286.1"
                     /translation="MNISEVDLHKLTVSDPFLGQYQQLVRDVVIPYQWDALNDRIPEA
                     EPSHAIENFRIAAGLQDGEFYGMVFQDSDVAKWLEAVAWSLCQKPDAELEKTADEVIE
                     LIASAQCEDGYLNAYFTVKAPEERWSNLAECHELYCAGHLIEAGVAFFQATGKRRLLE
                     VVCRLADHIDSVFGPGESKLHGYPGHPEIELALMRLYEVTEEPRYLALTNYFVEQRGA
                     QPHYYDQEYEKRGQTSHWHTYGPAWMVKDKAYSQAHLPIAQQQTAIGHTVRFVYLMTG
                     VAHLARLSHDESKRQDCLRLWNNMAQRQLYITGGIGSQSSGEAFSSDYDLPNDTVYAE
                     SCASIGLMMFARRMLEMEGDSQYADVMERALYNTVLGGMALDGKHFFYVNPLEVHPKT
                     LKFNHIYDHVKPIRQRWFGCACCPPNIARVLTSIGHYLYTPREDALYINIYAGNSMEV
                     PVENGTLRLRVSGNYPWQEQVTIAVESPQPVRHTLALRLPDWCTQPQIILNGEEVEQD
                     IRKGYLHITREWQEGDTLNLTLPMPVRRVYGNPLVRHVAGKVAIQRGPLVYCLEQADN
                     GESLHNLWLPADAPFTTFEGKGLFRHKILIQAPGYRYEQSNPEQQPLWHYDSAPAKRQ
                     PQTLTFIPWFSWANRGEGEMRIWVNEEKHCHPEVG"
     gene            complement(2830..4230)
                     /locus_tag="ACHKBL_003467"
     CDS             complement(2830..4230)
                     /locus_tag="ACHKBL_003467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO5820287.1"
                     /translation="MTSTPITAADIAKGKIDDALSVREKIGYGLGDAGGTVITCLIMN
                     FLTFFYTDVFGLTPALVGTLFIALRVFDAISDPVMGVIADRTQSRWGRFRPWQLWIAI
                     PIGIIGILTFTVPDASMGVKIAWAFGTYLLLSVGYTAINVPYCALINTMTTRHNEVIS
                     CQSWRFVLCGVAGFLVSVGLPWMVDLFGQGNAARGYQLGVGVLCAIAVVMFLCCFFWV
                     RERVPLSTMGKFTLREHLAGLRNNDQLLLMLVMSFLLINVFNIRGGGYMYFITYVLQG
                     STGYTSLFFTMVTFASIIGSVIVSPLTRRFDTVKIYYYTNLLLAALAVLMWFLPSGPA
                     YQTLWLAVILGNGVILGFTLPLHFSLMAFADDYGEWKTRVRSSGMNFAFNLFFIKLAW
                     ASSAGIISLLFIFVAYQPGVENQTTSSLGGITAMETLLPALFHLLLAGAIRFCKLNNP
                     MMSRIASDLRQRHVQP"
     gene            4456..5271
                     /locus_tag="ACHKBL_003468"
     CDS             4456..5271
                     /locus_tag="ACHKBL_003468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO5820288.1"
                     /translation="MLELSMTLPIKVQNGGLFISRGVGRHPARRLESWEIIFVEKGRL
                     TIQEEERIFLVDAGESLLLWPNRQHVGVEDFPADLKFYWLHFEVKAPDSDPRWLTHLS
                     VPQQTKVADPQALVALFRQFLSEQEKHQRTPALEFIVLLILQQLTRDARLDEQADAAG
                     TTLAWKAQKLIRTQYHRPLSTALLARELYCNADYLGRVYRRVFHLTLTEAIHRQRVRE
                     AEKLLISEARSLTEVAALCGFNDVGYFRQIFRKHTGLTPAAWKRRYSKEHINS"
     gene            complement(5303..5998)
                     /gene="araD"
                     /locus_tag="ACHKBL_003469"
     CDS             complement(5303..5998)
                     /gene="araD"
                     /locus_tag="ACHKBL_003469"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFO5820289.1"
                     /translation="MLEQLKADVLAANLALPAHHLVTFTWGNVSAVDEARQWMVIKPS
                     GVEYDVMTADDMVVVEIASGKVVEGSKKPSSDTPTHLALYRRYAEIGGIVHTHSRHAT
                     IWSQAGLDLPAWGTTHADYFYGAIPCTRQMTAEEINGEYEYQTGEVIIETFEERGRSP
                     AQIPAVLVHSHGPFAWGKNAADAVHNAVVLEECAYMGLFSRQLAPQLPAMQNELLDKH
                     YLRKHGANAYYGQ"
     gene            complement(5992..6852)
                     /locus_tag="ACHKBL_003470"
     CDS             complement(5992..6852)
                     /locus_tag="ACHKBL_003470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HFO5820290.1"
                     /translation="MRNHPLGIYEKALAKDLSWPERLVLAKSCGFDFVEMSVDETDER
                     LSRLDWSAAQRASLVTAMIETGVGIPSMCLSAHRRFPFGSRDEAVRERAREIMSKAIR
                     LARDLGIRTIQLAGYDVYYEDHDEGTRQRFAEGLAWTVEQAAASQVMLAVEIMDTAFM
                     NSISKWKKWDEMLASPWFTVYPDVGNLSAWGNDVPAELKLGIDRIAAIHLKDTQPVTE
                     QSPGQFRDVPFGEGCVDFVGIFKTLHELNYRGSFLIEMWTEKAKEPVLEIIQARRWIE
                     ARMQEAGFIC"
     gene            complement(6845..7507)
                     /gene="ulaD"
                     /locus_tag="ACHKBL_003471"
     CDS             complement(6845..7507)
                     /gene="ulaD"
                     /locus_tag="ACHKBL_003471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HFO5820291.1"
                     /translation="MSRPLLQLALDHSSLEDAQRDVTLLKDSVDIVEAGTILCLNEGL
                     GAVKALREQCPNKIIVADWKVADAGETLAQQAFGAGANWMTIICAAPLATVEKGHAMA
                     QRCGGEIQIELFGNWTLDDARDWHRIGVRQAIYHRGRDAQASGQQWGEADLARMKALS
                     DIGLELSITGGITPADLPLFKDIRVKAFIAGRALAGAANPVQVAGDFHAQIDAIWGGA
                     RA"
     gene            complement(7504..9000)
                     /gene="lyxK"
                     /locus_tag="ACHKBL_003472"
     CDS             complement(7504..9000)
                     /gene="lyxK"
                     /locus_tag="ACHKBL_003472"
                     /EC_number="2.7.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-xylulokinase"
                     /protein_id="HFO5820292.1"
                     /translation="MTQYWLGLDCGGSWLKAGLYDREGREAGVQRLPLCALSPQPGWA
                     ERDMAELWQCCMAVIRALLTHSGVSGEQIVGIGISAQGKGLFLLDKNDKPLGNAILSS
                     DRRAMEIVRRWQEDGIPEKLYPLTRQTLWTGHPVSLLRWLKEHEPERYAQIGCVMMTH
                     DYLRWCLTGVKGCEESNISESNLYNMSLGEYDPCLTDWLGIAEINHALPLVVGSAEIC
                     GEITAQTAALTGLKAGTPVVGGLFDVVSTALCAGIEDEFTLNAVMGTWAVTSGITRGL
                     RDGEAHPYVYGRYVNDGQFIVHEASPTSSGNLEWFTTQWGEISFDEINQAVASLPKAG
                     GDLFFLPFLYGSNAGLEMTSGFYGMQAIHTRVHLLQAIYEGVVFSHMTHLNRMRERFT
                     DVHTLRVTGGPAHSDVWMQMLADVSGLRIELPQVEETGCFGAALAARVGTGVYRDFSE
                     AQRDLQHPVRTLLPDMTAHQLYQQKYQRYQHLIAALQGFHARIKEHTL"
     gene            complement(9004..9990)
                     /gene="yiaO"
                     /locus_tag="ACHKBL_003473"
     CDS             complement(9004..9990)
                     /gene="yiaO"
                     /locus_tag="ACHKBL_003473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate TRAP transporter
                     substrate-binding protein YiaO"
                     /protein_id="HFO5820293.1"
                     /translation="MKLRSVTYALFIAGLAAFSTSSLAAQSLRFGYETSQTDSQHIAA
                     KKFNDLLQERTKGELKLKLFPDSTLGNAQAMISGVRGGTIDMEMSGSNNFAGLSPVMN
                     LLDVPFLFRDTAHAHKTLDGKVGDDLKASLEGKGLKVLAYWENGWRDVTNSRAPVKTP
                     ADLKGLKIRTNNSPMNIAAFKVFGANPIPMPFAEVYTGLETRTIDAQEHPINVVWSAK
                     FFEVQKYLSLTHHAYSPLLVVINKAKFDGLSPEFQQALVSSAQEAGNYQRKLVAEDQQ
                     KIIDGMKEAGVEVITDLDRKAFSDALGTQVRDMFVKDVPQGAELLKAVDEVQ"
     gene            complement(10003..11280)
                     /gene="yiaN"
                     /locus_tag="ACHKBL_003474"
     CDS             complement(10003..11280)
                     /gene="yiaN"
                     /locus_tag="ACHKBL_003474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate TRAP transporter large
                     permease YiaN"
                     /protein_id="HFO5820294.1"
                     /translation="MAVLIFLGCLLGGIAIGLPIAWALLLCGAALMFWLDMFDVQIMA
                     QTLVNGADSFSLLAIPFFVLAGEIMNAGGLSKRIVDLPMKLVGHKPGGLGYVGVLAAM
                     IMASLSGSAVADTAAVAALLVPMMRSANYPVNRAAGLIASGGIIAPIIPPSIPFIIFG
                     VSSGLSISKLFMAGIAPGMMMGATLMLTWWWQASRLNLPRQQKATMQEIWHSFVSGIW
                     ALFLPVIIIGGFRSGLFTPTEAGAVAAFYALFVATVIYREMTFATLWHVLIGAAKTTS
                     VVMFLVASAQVSAWLITIAELPMMVSDLLQPLVDSPRLLFIVIMVAILIVGMVMDLTP
                     TVLILTPVLMPLVKEAGIDPIYFGVMFIINCSIGLITPPIGNVLNVISGVAKLKFDDA
                     VRGVFPYVLVLYSLLVVFVFIPDLIILPLKWIN"
     gene            complement(11283..>11653)
                     /locus_tag="ACHKBL_003475"
     CDS             complement(11283..>11653)
                     /locus_tag="ACHKBL_003475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HFO5820295.1"
                     /translation="SVDELSRYLFVWLTFIGAIVAFMDNAHVQVTFLVEKLSPAWQRR
                     VALVTHSLILFICGALAWGATLKTIQDWSDYSPILGLPIGLMYAACLPTSLVIAFFEL
                     RHLYQLITRSNSLTSPPQGA"
BASE COUNT         2779 a         3133 c         3125 g         2616 t
ORIGIN      
        1 tggcgttatt tgcgttgcct tttatggcaa gcgcaactgc ttctgaatta tccattggtg
       61 ctggtgcggc ttataatgaa tcgccttatc gcggttataa tgaaaataca aaggcaattc
      121 cgctgattag ttatgaaggt gatacttttt atgttcgtca gaccacgtta ggttttattc
      181 tgtcgcaaag tgaaaaaaat gaacttagcc tgaccgcatc ctggatgccg ctggaatttg
      241 accctaccga taatgacgat tatgccatgc aacagcttga taagcgtgat agtacggcta
      301 tggcgggggt tgcctggtat caccacgagc gttggggaac cgtgaaagcc tctgcggctg
      361 cggacgttct ggataacagc aacggctggg tgggggagct atcggtattc cacaaaatgc
      421 agataggtcg cctgtcgctg acacctgcgc tgggcgttct ctattatgac gagaatttca
      481 gtgactatta ctatggcatt tcagagagtg agtcccgtcg tagcggtctg gcaagttatt
      541 ccgcgcagga tgcctgggtg ccttatgtca gcctgacggc aaaatacccg ataggagagc
      601 acgtcgtatt gatggcgagc gccggataca gcgagctgcc ggaagagatt accgacagcc
      661 cgatgattga tcgtaatgag agttttacct ttgtcaccgg cgtgagctgg cgtttttaat
      721 ttaccggtgg atgtcggtgc ggctggccta cgctctcgca ccgactcgtg ggcctgataa
      781 gacacgttaa gcgtcgcatc aggcaatgtg ctccctacgt tggatgcggc gcgagcgcct
      841 tatccaacct ccggatggca atgcttttcc tcattcaccc agatccgcat ttcgccttca
      901 ccccggttgg cccagctaaa ccacgggata aaggtcaggg tttgcggctg gcgtttggct
      961 ggcgcgctgt cgtaatgcca cagcggttgc tgctctggat tgctctgttc ataccgataa
     1021 cccggtgcct ggattaagat cttatggcgg aacaatccct taccttcaaa tgtcgtaaat
     1081 ggcgcatcgg cgggcagcca cagattatgc agtgactcgc cgttgtcggc ctgttccagg
     1141 caatacacca gcgggccgcg ctgaatcgcc acttttccgg cgacgtgacg caccagaggg
     1201 ttaccgtaaa cgcgacgtac cggcattggc aaagtcagat tcagcgtgtc gccctcctgc
     1261 cattcgcggg taatgtgcaa atacccttta cgaatatcct gctcgacctc ttccccattc
     1321 aatatgatct gcggctgtgt gcaccagtcc ggcagacgta aagccagcgt atgacgcacc
     1381 ggctggggcg attcaaccgc aattgtcacc tgttcctgcc acggatagtt tccgctaacc
     1441 cgcagacgca gcgtgccatt ttctaccggc acttccatgc tgtttcctgc gtagatgttg
     1501 atatacaacg catcttcacg cggcgtgtag agataatgac caatcgaggt tagcacgcgg
     1561 gcgatatttg gcggacaaca agcgcaacca aaccaacgct ggcggatcgg cttaacgtga
     1621 tcgtagatat ggttgaattt taacgttttt ggatgcactt ccagcggatt aacgtagaag
     1681 aaatgtttgc catccagcgc catgccgccg agcacagtgt tgtacagtgc gcgctccatc
     1741 acatcggcat attgactgtc gccttccatt tccagcattc gccgggcgaa catcatcagg
     1801 ccgatggaag cacaactttc ggcgtaaacc gtgtcattcg gcagatcgta atcgctgctg
     1861 aacgcttcgc cgctgctttg tgagccaatg ccgccggtaa tatataactg acgctgggcc
     1921 atattgttcc acagccgcag gcaatcctga cgcttgcttt catcgtgact taaacgcgcg
     1981 agatgtgcga cgccagtcat cagatataca aagcgtactg tatgtccgat ggcggtttgt
     2041 tgctgtgcga tgggcaaatg cgcctggctg taggctttat ctttcaccat ccacgccggg
     2101 ccgtaggtgt gccagtgcga ggtctgcccg cgcttttcat attcctggtc gtaatagtgc
     2161 ggttgcgcac cacgctgttc gacaaaatag ttcgtcagcg ccaggtagcg cggctcttcg
     2221 gtcacttcat acaggcgcat tagtgccagt tcaatttccg ggtgaccagg ataaccgtgt
     2281 aacttacttt cacctggacc aaatacgctg tcgatatgat cggccagacg gcaaaccact
     2341 tccagcaagc gccgttttcc cgtggcctgg aagaaggcga ctccggcttc aatcagatga
     2401 ccggcgcagt aaagttcatg acactccgcc agattgctcc agcgttcttc gggcgctttt
     2461 accgtaaagt aagcattgag atagccatct tcgcactggg cggaggcgat cagttcaatc
     2521 acctcgtcag cggttttttc cagttcggca tccggcttct ggcacagcga ccaggctacc
     2581 gcttccagcc atttggcgac gtcgctgtcc tgaaacacca tcccgtaaaa ttcaccgtct
     2641 tgcagtcctg cggcaatgcg aaagttttca atcgcatggc tgggttccgc ttctgggata
     2701 cgatcgttca aggcgtccca ctgataagga atcaccacat cgcggaccag ttgttggtac
     2761 tgaccgagga acggatcgct gaccgtcagt ttatgcagat cgacttccga aatgttcatc
     2821 ttgtgctcct taaggctgta catgacgctg acgcaggtcg ctagcaatgc gtgacatcat
     2881 agggttattg agtttgcaaa agcggatcgc ccctgccagc agcaggtgga atagcgcagg
     2941 cagtaatgtt tccatcgccg tgatcccgcc aagcgaactg gtggtctggt tttccacgcc
     3001 tggctggtag gcgacaaaaa taaacagcag gctgatgatc ccggcgctgg aggcccatgc
     3061 cagcttgatg aaaaacagat tgaaggcgaa gttcatgccg gaagaacgta cgcgggtttt
     3121 ccactcgccg tagtcatcgg caaaggccat caatgagaag tgcagtggca atgtgaagcc
     3181 aagaatcacg ccattaccga ggatcaccgc cagccacagc gtttgataag ccgggccaga
     3241 aggcaggaac cacatcaaca ccgccagcgc agcgaggagc aggttggtgt agtagtaaat
     3301 tttgacggta tcgaaacgcc gcgttaacgg gctgacaatc accgagccga taatggaggc
     3361 gaaggtgacc atggtgaaga acagcgacgt gtagcccgta ctgccttgta agacgtaggt
     3421 aatgaagtac atatacccac cgccgcgaat attaaagacg ttaatcagca ggaaagacat
     3481 gaccagcatc agcagcagtt ggtcgttgtt ccgcagcccg gcaagatgtt cgcgcagggt
     3541 aaatttcccc attgtggaga gtggcacccg ttcacgaacc cagaagaaac agcacaggaa
     3601 catcaccacg gcaatggcgc acagcacgcc gacgcccagt tgatagccgc gcgcggcatt
     3661 accctgaccg aagagatcca ccatccacgg caggccaacc gaaaccagaa atcccgctac
     3721 gccgcagaga acgaatcgcc aggactggca ggagatcact tcattgtggc gggtggtcat
     3781 ggtgttgatc agcgcgcagt acggtacgtt gatggcggta taaccgactg aaaggagcag
     3841 gtaagtaccg aacgcccagg cgatttttac tcccatgctg gcatctggca cggtgaacgt
     3901 caggatgccg ataatgccga tgggaatggc aatccataac tgccacggac gaaagcgccc
     3961 ccagcgactt tgcgtccggt cggcaatgac gcccatcacc gggtcggaga tggcatcaaa
     4021 cacgcgcagt gcaataaaca gcgtgccgac cagcgccgga gttaagccaa aaacgtcggt
     4081 gtagaaaaag gtgagaaagt tcatgatcag gcaagttatt acggtgccgc ctgcgtcacc
     4141 caggccgtag cctatttttt cccgtacaga taacgcatcg tcaattttac ctttagcgat
     4201 atcagcggca gtaatcggtg tagaggtcat agatattcct cgtgaatagg gagcaatttt
     4261 tagttttatt gcaatgacct gattaattct gccgtgaacg ggaatatcaa caaggcgaga
     4321 aaagagtaaa aaaaggtgac gattccgacc tgaatttaaa agtgtgaaat ggatcaggtg
     4381 aaattaatgt ttttgattaa taatcaaaag gtaatgtgtt taatcattaa tgaataagcg
     4441 ggaaattaca tatcaatgct cgaattatcc atgacgttac cgattaaagt gcaaaatggt
     4501 ggattattta tttcccgtgg cgtagggagg cacccggcaa ggcggctgga gtcatgggaa
     4561 attatctttg tcgaaaaagg tcgattaacg attcaggaag aagagcggat atttctggtc
     4621 gatgcgggag agagtttatt actctggccg aaccgacagc atgttggggt agaggatttt
     4681 cccgccgatc tcaaatttta ctggctgcat tttgaggtta aagcgccgga tagcgacccg
     4741 cgctggctca ctcatcttag cgtgccgcaa caaacgaagg ttgccgatcc gcaggcgctg
     4801 gtggccctgt ttcgccagtt cttaagcgaa caggaaaagc atcaacgcac tccggcgctg
     4861 gagtttattg tgctgctgat tttgcagcag ctcacccgtg acgcgcgtct tgatgaacag
     4921 gccgatgccg caggtaccac gcttgcctgg aaagcgcaga aactgattcg cacgcaatat
     4981 catcgcccgc tttctacggc gcttttagcc cgcgagctgt actgcaatgc cgattatctt
     5041 gggcgcgtct accggcgggt gtttcatctg acgttaacag aagcaattca tcgccagcga
     5101 gtgcgcgaag ccgaaaagct gttaattagc gaagcgcgat cgctcactga agtggctgcg
     5161 ttgtgcgggt ttaatgacgt tggctatttc cgacagattt ttcgcaaaca caccggttta
     5221 acgccagctg cgtggaagcg acgttacagc aaggaacata tcaattcgta gtgccggggc
     5281 aatgaagccc cggcgtgagg gattactgcc cgtaataggc attggcccca tgcttacgca
     5341 ggtagtgctt atctaacagt tcgttttgca tcgcagggag ctgcggcgca agctggcgcg
     5401 agaacaggcc catataggcg cactcttcca gcactacggc attatgcacc gcatcggcgg
     5461 cattttttcc ccaggcgaac gggccgtgag aatgcaccag caccgccggg atttgtgccg
     5521 gactcctgcc gcgttcttcg aaagtttcaa tgatcacttc gccggtctga tattcatatt
     5581 cgccgttaat ctcctctgcg gtcatctgtc gcgtgcaggg gatggcacca tagaaataat
     5641 cggcgtgcgt ggtgccccag gcggggagat ccagcccggc ctgcgaccag atggtggcgt
     5701 ggcgcgagtg ggtatgcaca atgccaccga tttcggcata gcgacggtaa agcgccagat
     5761 gcgttggcgt gtcggacgag ggctttttgc tgccttccac caccttaccg ctggctatct
     5821 caaccaccac catatcgtcg gcggtcatca cgtcgtactc gacgccggaa ggtttgatta
     5881 ccatccattg ccgcgcttcg tctaccgcgc tgacattgcc ccaggtgaac gtcaccagat
     5941 ggtgagcggg aagtgccaga tttgccgcca gcacgtcggc tttcagttgc tctaacatat
     6001 aaaacctgcc tcctgcatac gcgcttcaat ccagcgccgc gcctgaataa tctccagcac
     6061 cggctctttg gctttttcgg tccacatctc aatcaggaaa gaaccgcgat agttcagttc
     6121 atgcagcgtt ttgaagatgc caacgaaatc gacgcagcct tcgccaaacg gcacatcacg
     6181 gaactgtccg gggctttgct cggtgactgg ttgagtatct ttcaggtgga tcgcggcgat
     6241 acggtcaatg cccagtttca gttcggcggg aacatcattg ccccaggcgc tgaggttgcc
     6301 gacgtccggg tagacggtga accacggcga ggcgagcatc tcgtcccact ttttccattt
     6361 gctgatggag ttcataaacg cagtatccat aatctccacc gccagcatta cttgtgatgc
     6421 cgccgcctgt tcgactgtcc acgccagccc ttcagcaaaa cgttgccggg tgccttcgtc
     6481 gtggtcttcg taatagacgt cgtagcccgc cagctggatg gtgcgaatgc cgagatcgcg
     6541 cgccaggcga atcgctttac tcatgatttc ccgcgcccgt tcgcgcactg cctcgtcacg
     6601 gctaccaaaa ggaaagcgac gatgtgcgga caggcacatc gacgggatcc caacacccgt
     6661 ttcgatcatc gcagtgacca gcgaagcacg ttgtgcggcg ctccagtcaa gacgtgagag
     6721 ccgttcgtcg gtttcatcca ccgacatttc gacaaaatca aagccacagc tttttgccag
     6781 caccagccgc tccggccagg agagatcttt cgccagcgct ttttcataaa tccctaacgg
     6841 atgattacgc acgcgcacct ccccagatgg catcaatttg tgcatggaaa tcgccagcca
     6901 cctgcaccgg atttgctgcg cctgccagtg ctcgcccggc aataaacgct ttcacgcgaa
     6961 tatctttaaa cagcggcagg tcagcagggg taatcccgcc ggtaatggaa agctcaaggc
     7021 cgatatcgga aagcgccttc atgcgtgcca gatcggcctc gccccactgt tgcccgctgg
     7081 cctgcgcgtc gcgaccgcga tgataaatgg cctgccgcac gccaatacga tgccagtcgc
     7141 gggcgtcgtc cagcgtccag ttaccgaaca actcgatctg aatttcacca ccgcagcgtt
     7201 gcgccatcgc gtggcctttt tccaccgtcg cgagcggtgc ggcgcagatg atggtcatcc
     7261 agttagcgcc tgcgccaaac gcctgttgcg cgagcgtttc accggcatcg gcgaccttcc
     7321 agtcagcgac gatgattttg ttcgggcact gttcgcgcaa ggctttcact gcgccaagcc
     7381 cttcgtttaa acagagaatg gtgcccgctt cgacgatatc gacgctgtct tttaacagcg
     7441 tcacgtcgcg ctgtgcgtct tcaagtgatg agtggtcgag ggccagttgc agaagtggtc
     7501 ggctcataat gtgtgctcct tgatgcgggc gtgaaagccc tgaagtgcgg caatgagatg
     7561 ctgataacgt tggtattttt gttggtaaag ctgatgggcg gtcatatccg gcagcagggt
     7621 gcgtaccggg tgctgtaaat cacgttgggc ttcgctgaaa tcgcgataaa ctccggtgcc
     7681 gacgcgggcg gcaagtgccg caccaaagca gccggtttct tccacctgcg gcagctcgat
     7741 acgcagaccg ctgacgtccg ccagcatttg catccagaca tcggagtgcg ccggaccgcc
     7801 agtgacgcgc agggtgtgca catcagtaaa acgttcgcgc atccggttga gatgggtcat
     7861 atggctgaac accacgcctt cataaatggc ctgcaacagg tgtacgcggg tgtgaatggc
     7921 ctgcatcccg tagaaaccgc tggtcatctc gagtccagcg ttgctgccgt acaggaacgg
     7981 caggaaaaag agatcgcccc cggccttcgg caagctggca acggcctggt tgatctcatc
     8041 aaacgagatt tctccccact gtgtggtaaa ccattcgaga ttgccggaag aggtggggct
     8101 tgcttcatga acgataaatt gaccatcgtt aacgtagcga ccatagacat acggatgcgc
     8161 ttcaccgtca cgtaaaccac gggttatgcc gctggtcacc gcccaggtgc ccatcaccgc
     8221 attgagggta aattcgtctt cgatcccggc gcagagtgcg gtggaaacca catcaaacag
     8281 gccgccgaca acgggcgtac ccgctttcag accggtcagt gcggctgtct gagcggtgat
     8341 ctccccgcag atttcggcag atccgacaac aagtggcagg gcgtggttga tttcagcgat
     8401 ccccagccag tcggtgaggc acgggtcata ttccccaaga ctcatgttgt agagattgga
     8461 ctcagaaata ttgctctctt cgcaaccttt gacgccggtt aaacaccagc gcaggtagtc
     8521 gtgcgtcatc atcacgcagc caatttgcgc gtagcgttcc ggttcgtgct ctttcagcca
     8581 gcgtaacagc gacaccggat gcccggtcca caaggtttgt cgcgtcagcg ggtagagttt
     8641 ttccgggatg ccatcttcct gccagcgacg aacgatttcc atcgcccggc gatccgagga
     8701 caaaatggcg ttaccgagcg gcttgtcgtt tttatccagc aaaaacaagc cctttccctg
     8761 tgcggagatg ccgataccga caatttgttc cccgctaaca ccagaatgag taagcagggc
     8821 gcgaatgaca gccatgcagc attgccacag ttctgccata tcgcgctctg cccaacctgg
     8881 ctgcgggctt aatgcgcaca gcggcaggcg ctgcacgcct gcctcccggc cttcgcggtc
     8941 atacagcccg gctttcagcc agctaccgcc acaatctaac cccagccagt attgcgtcat
     9001 ggtttattgc acctcatcca cggctttcag cagctcagcg ccctgcggaa catctttaac
     9061 aaacatgtcg cggacctgag tacccagtgc gtcgctaaag gctttgcggt cgagatcggt
     9121 gatgacttcc acgcccgctt ctttcatgcc gtcgatgatt ttttgctgat cttcggcaac
     9181 cagtttgcgc tggtagttac ccgcttcctg tgcagatgaa actagcgcct gctggaactc
     9241 cgggcttaag ccatcaaact tcgctttgtt gatcaccacc agaagtggcg aataggcgtg
     9301 gtgcgtcaga gaaaggtact tctgtacttc gaaaaatttt gctgaccaga cgacgttgat
     9361 cgggtgttcc tgagcgtcga tagtgcgcgt ttccagcccg gtatagactt cggcaaacgg
     9421 catcgggatc gggttagcgc caaagacttt gaatgcggcg atattcatcg ggctattgtt
     9481 ggtgcgaatt ttcagccctt tcaggtcggc gggggtttta accggtgcgc gcgagttggt
     9541 gacatcgcgc cagccgtttt cccagtaggc cagtactttt agtcctttac cttcaagtga
     9601 ggctttcaga tcatcaccga ctttgccgtc gagcgtttta tgcgcgtgag cggtatcgcg
     9661 gaacaggaaa gggacatcaa gcaagttcat cactggtgat aacccggcaa agttattcga
     9721 gccggacatt tccatatcga tggtgccgcc acgtacgccg ctgatcatcg cctgtgcgtt
     9781 accgagagta ctgtccggga acagttttaa tttcagctcg cctttggttc tctcctgcaa
     9841 taaatcattg aattttttcg ccgcaatatg ttgcgagtcg gtttgtgagg tttcataacc
     9901 gaaacgtaaa gattgcgccg ccagtgaaga tgtactaaat gcagccaggc cagcaatgaa
     9961 taatgcgtag gttacagagc gtaatttcat aatattttcc ttttaattaa tccatttcaa
    10021 agggaggatg atgagatcgg gaataaaaac aaatacgact aataatgaat ataaaaccag
    10081 gacgtaaggg aaaacgcctc tgactgcatc atcgaatttg agttttgcca cccctgaaat
    10141 aacgttaagc acattaccga taggcggtgt aattaagccg attgagcagt taatgataaa
    10201 catgacaccg aaataaatcg gatcgattcc tgcctcttta actaaaggca ttaataccgg
    10261 ggtaagaatt aataccgtcg gcgttaaatc catgaccatg ccgacaatca ggatagccac
    10321 cataatgacg ataaacagca gacgcggtga atcgaccagc ggttgcagta aatcagaaac
    10381 catcatcggc agttcagcaa tggtgatcag ccaggcggaa acttgtgccg aggctaccag
    10441 aaacatcacc actgaggtcg ttttcgctgc gccaatgagc acatgccaaa gtgtggcgaa
    10501 ggtcatttca cggtaaataa ctgtggcgac aaacagcgca tagaaagcag caacggcccc
    10561 ggcttcggta ggggtaaaca gaccggagcg aaacccgcca ataatgatga ctggaagaaa
    10621 cagcgcccag ataccggaga caaaagagtg ccagatttcc tgcatcgttg ctttttgctg
    10681 gcggggtaaa ttaaggcgac tggcctgcca ccaccaggta agcatcagcg ttgcgcccat
    10741 catcatgcca ggggcaatgc ccgccataaa cagcttactg atggataatc cgctggaaac
    10801 gccgaaaata ataaacggaa tggaaggggg aataattggc gcgataatgc cgccagaggc
    10861 aatcagcccc gcagcccggt tgaccgggta gttagcgctg cgcatcatcg gcactaatag
    10921 cgcggcaacg gcggcggtat cagcaacagc agaaccagaa aggctcgcca taatcatagc
    10981 cgccagcacg ccgacgtagc ccaacccgcc aggtttatgc cccaccagtt tcatcggcag
    11041 gtcaacaatg cgttttgaca agccgcccgc attcatgatt tcacccgcca gcacaaagaa
    11101 cggaatcgcc agcagggaga agctgtcggc tccgttcacc agcgtttgtg ccatgatctg
    11161 gacatcaaac atgtccagcc agaacattaa cgccgctccg cacaacaaca gtgcccaggc
    11221 aataggcaaa ccgatagcaa taccacccaa cagacagccc agaaaaatca gcacagccat
    11281 gattaagctc cttgcggtgg cgacgttaga gaattgctac gcgtgatgag ttgatataaa
    11341 tgacgcagtt caaagaatgc gataacgagg ctggtgggca gacaagcggc atacatcagg
    11401 ccgatgggca aaccgaggat cggtgaataa tcgctccagt cctgaattgt ttttagcgtt
    11461 gcgccccagg ccagtgcgcc acaaataaat aagattaagg aatgggtaac cagagcgact
    11521 cgtcgctgcc atgcggggga gagtttctcc accagaaagg tgacctgaac gtgggcgtta
    11581 tccataaaag ccacaatcgc gccaataaat gttaaccaga caaataaata acgtgacaat
    11641 tcatctacag ata
//
LOCUS       DAZFLD010000148        11629 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.149, whole genome shotgun sequence.
ACCESSION   DAZFLD010000148 DAZFLD010000000
VERSION     DAZFLD010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11629
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.149"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            55..459
                     /gene="yaaI"
                     /locus_tag="ACHKBL_003476"
     CDS             55..459
                     /gene="yaaI"
                     /locus_tag="ACHKBL_003476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HFO5820296.1"
                     /translation="MKSVFTLSASLAISLLLCCTAQANDHKILGVIAMPRNETNDLAL
                     KLPVCRIVKRIQLSADHGDLQLSGASVYFKAARSASQTLNIPSEIKEGQTTDWININS
                     DNDNKRCVSKITFSGHTVNSSDMATLKIIGDD"
     gene            485..1198
                     /gene="msyB"
                     /locus_tag="ACHKBL_003477"
     CDS             485..1198
                     /gene="msyB"
                     /locus_tag="ACHKBL_003477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HFO5820297.1"
                     /translation="MNVNYLNDSDLDFLQHCSEEQLANFARLLTHNEKGKTRLSSVLM
                     RNELFKSMEGHPEQHRRNWQLIAGELQHFGGDSIANKLRGHGKLYRAILLDVSKRLKL
                     KADKEMSTFEIEQQLLEQFLRNTWKKMDEEHKQEFLHAVDARVNELEELLPLLMKDKL
                     LAKGVSHLLSSQLTRILRTHAAMSVLGHGLLRGAGLGGPVGAALNGVKAVSGSAYRVT
                     IPAVLQIACLRRMVSATQV"
     gene            1347..1913
                     /gene="satP"
                     /locus_tag="ACHKBL_003478"
     CDS             1347..1913
                     /gene="satP"
                     /locus_tag="ACHKBL_003478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HFO5820298.1"
                     /translation="MGNTKLANPAPLGLMGFGMTTILLNLHNVGYFALDGIILAMGIF
                     YGGIAQIFAGLLEYKKGNTFGLTAFTSYGSFWLTLVAILLMPKLGLTDAPNAQFLGVY
                     LGLWGVFTLFMFFGTLKGARVLQFVFFSLTVLFALLAIGNIAGNAAIIHFAGWIGLIC
                     GASAIYLAMGEVLNEQFGRTVLPIGESH"
     gene            complement(1948..2535)
                     /gene="mog"
                     /locus_tag="ACHKBL_003479"
     CDS             complement(1948..2535)
                     /gene="mog"
                     /locus_tag="ACHKBL_003479"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HFO5820299.1"
                     /translation="MNTLRIGLVSISDRASSGVYQDKGIPALEEWLTSALTTPFELET
                     RLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQ
                     MRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAAGNVVVHGIFA
                     SVPYCIQLLEGPYIETAPEVVAAFRPKSARRDVSE"
     gene            complement(2650..3603)
                     /gene="tal"
                     /locus_tag="ACHKBL_003480"
     CDS             complement(2650..3603)
                     /gene="tal"
                     /locus_tag="ACHKBL_003480"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HFO5820300.1"
                     /translation="MTDKLTSLRQYTTVVADTGDIAAMKLYQPQDATTNPSLILNAAQ
                     IPEYRKLIDDAVAWAKQQSNDRAQQIVDATDKLAVNIGLEILKLVPGRISTEVDARLS
                     YDTEASIAKAKRLIKLYNDAGISNDRILIKLASTWQGIRAAEQLEKEGINCNLTLLFS
                     FAQARACAEAGVFLISPFVGRILDWYKANTDKKEYAPAEDPGVVSVSEIYQYYKEHGY
                     ETVVMGASFRNIGEILELAGCDRLTIAPALLKELAESEGAIERKLSYTGEVKARPARI
                     TESEFLWQHNQDPMAVDKLAEGIRKFAVDQEKLEKMIGDLL"
     gene            3882..5312
                     /gene="yaaJ"
                     /locus_tag="ACHKBL_003481"
     CDS             3882..5312
                     /gene="yaaJ"
                     /locus_tag="ACHKBL_003481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HFO5820301.1"
                     /translation="MPDFFSFINSVLWGSVMIYLLFGAGCWFTFRTGFVQFRYIRQFG
                     KSLKNSIHPQPGGLTSFQSLCTSLAARVGSGNLAGVALAITTGGPGAVFWMWVAAFIG
                     MATSFAECSLAQLYKERDVNGQFRGGPAWYMARGLGMRWMGVLFALFLLIAYGIIFSG
                     VQANAVARALSFSFDFPPLVTGIILAVFALLAITRGLHGVARLMQGFVPLMAIIWVLT
                     SLVICVMNIGQLPHVIWSIFESAFGWQEAAGGAAGYTLSQAITNGFQRSMFSNEAGMG
                     STPNAAAAAASWPPHPAAQGIVQMIGIFIDTLVICTASAMLILLAGNGTTYMPLEGIQ
                     LIQKAMRVLMGSWGAEFVTLVVILFAFSSIVANYIYAENNLFFLRLNNPKAIWCLRIC
                     TFATVIGGTLLSLPLMWQLADIIMACMAITNLTAILLLSPVVHTIASDYLRQRKLGVR
                     PVFDPLRYPDIGRQLSPDAWDDVSQE"
     gene            5382..6158
                     /gene="yaaA"
                     /locus_tag="ACHKBL_003482"
     CDS             5382..6158
                     /gene="yaaA"
                     /locus_tag="ACHKBL_003482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HFO5820302.1"
                     /translation="MLILISPAKTLDYQSPLPTTRYTLPELLDNSQQLIHEARKLTPP
                     QISTLMRISDKLAGINAARFHDWQPDFTPENARQAILAFKGDVYTGLQAETFSEDDFD
                     FAQQHLRMLSGLYGVLRPLDLMQPYRLEMGIRLENARGKDLYQFWGDIITNKLNEALA
                     AQGDNVVINLASDEYFKSVKPKKLNAEIIKPVFLDEKNGKFKIISFYAKKARGLMSRF
                     IIENRLTKPEQLTGFNSEGYFFDEASSSNGELVFKRYEQR"
     gene            complement(6198..6494)
                     /locus_tag="ACHKBL_003483"
     CDS             complement(6198..6494)
                     /locus_tag="ACHKBL_003483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HFO5820303.1"
                     /translation="MKKMQSIVLALSLVLVAPMAAQAAEMTLVPSVKLQIGDRDNRGY
                     YWDGGHWRDHGWWKQHYEWRGNRWHPYGPPPSPRHNKHNDHRGDHRPGPDKHHR"
     gene            complement(6708..7994)
                     /gene="thrC"
                     /locus_tag="ACHKBL_003484"
     CDS             complement(6708..7994)
                     /gene="thrC"
                     /locus_tag="ACHKBL_003484"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HFO5820304.1"
                     /translation="MKLYNLKDHNEQVSFAQAVTQGLGKNQGLFFPHDLPEFSLTEID
                     EMLKLDFVTRSAKILSAFIGDEIPQEILEERVRAAFVFPAPVANVESDVGCLELFHGP
                     TLAFKDFGGRFMAQMLTHIAGDKPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRG
                     KISPLQEKLFCTLGGNIETVAIDGDFDACQALVKQAFDDEELKVALGLNSANSINISR
                     LLAQICYYFEAVAQLPQEARNQLVVSVPSGNFGDLTAGLLAKSLGLPVKRFIAATNVN
                     DTVPRFLHDGQWSPKATQATLSNAMDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDE
                     TTQQTMRELKELGYTSEPHAAVAYRALRDQLHPGEYGLFLGTAHPAKFKESVEAILGE
                     TLDLPKELAERADLPLLSHNLPADFAALRKLMMNHQ"
     gene            complement(7995..8927)
                     /gene="thrB"
                     /locus_tag="ACHKBL_003485"
     CDS             complement(7995..8927)
                     /gene="thrB"
                     /locus_tag="ACHKBL_003485"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HFO5820305.1"
                     /translation="MVKVYAPASSANMSVGFDVLGAAVTPVDGALLGDVVTVEAAETF
                     SLNNLGRFADKLPSEPRENIVYQCWERFCQELGKQIPVAMTLEKNMPIGSGLGSSACS
                     VVAALMAMNEHCGKPLNDTRLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDI
                     ISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQ
                     PELAAKLMKDVIAEPYRERLLPGFRQARQAVAEIGAVASGISGSGPTLFALCDKPDTA
                     QRVADWLGKNYLQNQEGFVHICRLDTAGARVLEN"
     gene            complement(8929..11391)
                     /gene="thrA"
                     /locus_tag="ACHKBL_003486"
     CDS             complement(8929..11391)
                     /gene="thrA"
                     /locus_tag="ACHKBL_003486"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009067 - aspartate family amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HFO5820306.1"
                     /translation="MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKIT
                     NHLVAMIEKTISGQDALPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIK
                     HVLHGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY
                     LESTVDIAESTRRIAASRIPADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTGNPQAPGTLIGASRDEDELPVKGISNLNNMAMFSVSGPGMKGMVGMAAR
                     VFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAV
                     TERLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATT
                     GVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNKHIDLRVCGVANSKA
                     LLTNVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYAD
                     FLREGFHVVTPNKKANTSSMDYYHLLRHAAEKSRRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELMKFSGILSGSLSYIFGKLDEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARK
                     LLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMANLSQLDDLFAARVAKARDEG
                     KVLRYVGNIDEDGVCRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAG
                     NDVTAAGVFADLLRTLSWKLGV"
     misc_feature    complement(11417..11536)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00506"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Thr leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00506"
     gene            complement(11472..11537)
                     /gene="thrL"
                     /locus_tag="ACHKBL_003487"
     CDS             complement(11472..11537)
                     /gene="thrL"
                     /locus_tag="ACHKBL_003487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thr operon leader peptide"
                     /protein_id="HFO5820307.1"
                     /translation="MKRISTTITTTITITTGNGAG"
BASE COUNT         2711 a         3124 c         2911 g         2883 t
ORIGIN      
        1 agcagtaaac tagtgggtat tcatccccta cctctcccca ctaagagaat ccttatgaaa
       61 tccgttttta cgctttccgc cagcctggcg attagcctgc tgctgtgctg cacggcgcag
      121 gcaaacgacc ataaaatcct cggcgtcatt gcaatgccac gtaacgaaac caacgatctg
      181 gcgctgaaac ttcctgtttg ccgaattgtt aaacgcatac aactctcagc cgaccatggc
      241 gatttacagt taagcggtgc atcggtttat ttcaaagccg cccgtagcgc cagtcagacc
      301 ctgaatattc cttcagaaat aaaagaaggg caaaccactg actggatcaa cattaacagc
      361 gataacgaca ataaacgctg cgtctcaaaa atcacctttt ctggtcatac ggtgaactca
      421 tcggatatgg ccacgctgaa aattatcggc gacgactaac gccagaatat agaagccaca
      481 aaaaatgaat gttaattacc tgaatgattc agatctggat tttctccagc attgtagtga
      541 ggaacaactg gcaaatttcg cccggctgct cacccataat gaaaaaggca aaactcgcct
      601 ctccagcgta ctgatgcgca acgaactgtt taaatcgatg gaaggacatc ccgagcaaca
      661 ccgccgcaac tggcagctga ttgccggaga attacaacat tttggtggcg atagtatcgc
      721 caacaaactg cgcggacacg gtaaattgta tcgggccatt ttgctcgatg tttcaaaacg
      781 attgaagctg aaagccgaca aagagatgtc tacgtttgaa attgagcaac agttactgga
      841 acaatttctg cgtaatacct ggaagaaaat ggacgaggaa cataagcagg agtttctgca
      901 cgcggtcgat gccagggtga atgagctgga agagctgctg ccgctgctga tgaaagacaa
      961 attattggca aaaggcgtgt cgcatttgct ttccagccaa ttgacccgca ttttacgcac
     1021 ccacgcagca atgagcgtac ttgggcatgg tttgcttcgc ggcgcggggc tgggaggccc
     1081 tgtaggcgcg gcactaaatg gggttaaagc ggtcagcggc agtgcctatc gcgtgacaat
     1141 tccagccgta ctgcaaatcg cctgcctgcg ccggatggtt agtgccactc aggtctgata
     1201 ccggaaaccg gggaaaattt tcattttccc ctcaaaagat cgtagacact gccccactgg
     1261 ctgattatta tgccgcgccc tgaaaacact acagttattc agggaaatta tttcaccatt
     1321 cattcgatga tgatttttga ggaattatgg gcaacactaa gttggctaat ccggcaccgc
     1381 tgggcctgat gggcttcggc atgaccacca ttctgcttaa cctgcacaac gtgggttatt
     1441 ttgctctgga cggtattatt cttgccatgg gcattttcta cggcggcatc gcgcaaattt
     1501 ttgctggtct gctggagtac aaaaaaggca acactttcgg tttaaccgca ttcacctctt
     1561 acggttcttt ctggctgacg ctggttgcga ttctgctgat gccgaaactg ggtctgaccg
     1621 atgcgccaaa tgcacagttc cttggtgtct acctgggtct gtggggcgta tttacgctgt
     1681 ttatgttctt cggcacgctg aaaggcgcac gcgttctgca attcgttttc tttagcctga
     1741 ccgtgctgtt tgccctgctg gcgatcggta acattgccgg taacgccgca atcatccact
     1801 ttgccggctg gattggtctg atctgcggtg ccagcgcaat ctatctggcg atgggtgaag
     1861 tactgaacga gcagtttggt cgcaccgttc tgccgattgg tgaatcccat taattgtttt
     1921 gagatccccc cgctcggggg gattttttta ttcgctaacg tcgcgtcttg cactcttcgg
     1981 tctgaatgct gcaaccactt ccggtgccgt ttcaatgtat ggcccttcca gcaactgaat
     2041 gcagtacggt acgctggcaa aaataccatg caccacaacg ttacccgcag cgtccttcac
     2101 gccttccagc gtctctttaa tcgacttcgg ctgaccgggt aagttaagga tcagcgcctg
     2161 tttgcgaatc acgcccacct gacgcgaaag gatcgcagtt ggtacaaaat gcaggctgat
     2221 ctggcgcatc tgttcaccaa agccaggcat ctcgcggtcc gctactgcca gcgtcgcatc
     2281 gggcgttacg tcacgacgcg ccgggccagt tccgcccgtg gtgagcacca gatggcaact
     2341 catttcatcc accagctcac acaacgtttg ctcgatgatc gcctgctcat cggggattaa
     2401 gcgggtttcc agttcaaacg gcgtggttag cgccgatgtc agccattctt ccagcgcagg
     2461 gatgccttta tcctgataaa cgccgctgga tgcgcgatca gagatggaaa ctaagccaat
     2521 acgtaaagta ttcatcaaga tttttccgtt taaacagtct cgttaaacag aatgatacac
     2581 tgcgaaggga gtgacagaca ggcttcagaa aaggtagcgt gaccgacttc ccggtcacgc
     2641 taagaatgat tacagcagat cgccgatcat tttttccagt ttttcctggt caacagcaaa
     2701 cttacggata ccttccgcca gtttatctac tgccattgga tcctggttat gctgccacag
     2761 gaactcggac tcagtgatac gcgccggacg cgctttcact tcgccggtgt aagacagttt
     2821 acgttcgata gccccttcgc tctcagccag ctctttcagc agcgccggtg cgatggtcag
     2881 acggtcgcag cctgccagtt ccagaatttc gccgatgtta cggaagcttg cgcccatcac
     2941 cacggtttca taaccgtgct ctttgtagta ctggtagatt tcagatacag aaaccacgcc
     3001 cggatcttct gccggagcgt actctttctt gtcggtattc gctttgtacc agtcaagaat
     3061 acggccaaca aacggcgaga tcaggaacac gcccgcttcc gcacaagcac gagcctgagc
     3121 gaaggagaac agcagggtca ggttacagtt aataccttct ttttccagct gttctgcagc
     3181 acggataccc tgccaggtag aagccagttt gatcagaata cgatcgttgc taatccctgc
     3241 atcgttgtag agtttgatca ggcgttttgc tttcgcaatt gacgcttcgg tgtcatagga
     3301 aagacgcgca tcaacttcag ttgagatacg gcccggaacc agtttcagga tttccagacc
     3361 aatatttact gccagtttgt cggtcgcgtc cacgatctgc tgcgcgcgat cgttgctctg
     3421 ctgtttcgcc caggcgacag catcatcaat caacttacgg tattccggaa tctgcgctgc
     3481 gttaagaatg agagaagggt tggttgtggc atcctgcggt tgatacagct tcattgccgc
     3541 gatgtcccca gtgtcggcca ctacggtggt gtactgacga agggaggtca atttgtccgt
     3601 catgatagta tttctcttta aacagcttgt tagggggatg taaccggtct gccctgatga
     3661 tatcacgacg caacgtcagc gcaaccgccg acaagacggt tatcgccagg cgagactgtt
     3721 tcggatttct gacactgcgt tgatatcgcc cgccattttt atacaaaacc tcatgtatgc
     3781 tacgcagaag ttatcaagta cctcgtagcg tatatacttc ttaaacaatt ggtaacgttt
     3841 acacaggaaa gtcatcgcga ccggcaataa gagggatatg catgccagat tttttctcct
     3901 ttattaacag cgtcctttgg ggatcggtaa tgatttacct gctcttcggc gcaggttgtt
     3961 ggttcacttt tcgcaccgga tttgtgcagt ttcgctacat ccgccagttt ggcaaaagtc
     4021 ttaaaaatag cattcatcca cagccaggcg gtttaacctc atttcagtca ttgtgtacca
     4081 gtcttgcggc gcgcgtgggt agcggcaacc tggctggcgt tgcgctggct attaccaccg
     4141 gtggaccagg tgccgtcttc tggatgtggg ttgccgcgtt tatcggcatg gcgacctcgt
     4201 ttgccgaatg ttcccttgca caactttata aagaacgtga cgttaatggg cagtttcgtg
     4261 gcggaccggc atggtatatg gcgcgcgggc tggggatgcg ctggatgggc gttctgttcg
     4321 ccctcttttt gctcatcgcc tacggcataa ttttcagcgg agttcaggcg aacgccgttg
     4381 cccgagccct gagtttttct tttgattttc ccccgctggt gacaggcatt attcttgctg
     4441 tctttgctct gctggcaatc actcgcggtc ttcatggcgt cgcccggctc atgcaggggt
     4501 ttgtcccgtt gatggcgata atctgggtac tgaccagcct ggtaatatgc gtaatgaata
     4561 tcgggcaact tccccacgtc atttggtcta tttttgagag tgcttttggc tggcaggaag
     4621 cggcaggcgg cgcggcggga tataccttaa gccaggcgat tactaacggt tttcagcgca
     4681 gtatgttttc caatgaggcg ggaatggggt cgacgccaaa cgcggcagcg gcggctgcgt
     4741 cctggcctcc gcatcccgca gcgcaaggaa ttgtccagat gattggcatt tttatcgaca
     4801 ccctggtcat ctgtacggca agcgccatgc tgatattact ggcgggtaac ggcacaacct
     4861 acatgccgct ggaaggtatt cagcttatcc agaaggcgat gcgggtactc atgggttcct
     4921 ggggtgctga gtttgttacc ctcgtggtta ttctgtttgc cttcagctcc atcgttgcca
     4981 actacattta tgccgaaaac aatctcttct tcttacgcct gaacaaccct aaagcgatct
     5041 ggtgtctgcg gatctgcacc ttcgcaacgg tcatcggcgg caccttgcta agtcttccgc
     5101 tgatgtggca actggcagat atcataatgg cctgcatggc tattaccaat ttgaccgcca
     5161 tattactgct ctcgcctgtg gttcatacca ttgccagtga ttatctacgc cagcgtaaac
     5221 tcggcgtgcg cccggtgttt gatccgttgc gttatccgga tatcggtcgc cagctttctc
     5281 cggacgcgtg ggatgatgtt tcgcaggagt aatcacaact atcgatcaac tcattctctt
     5341 tttttgctaa agtcggcata aatttcctgc aaggactgga tatgctgatt cttatttcac
     5401 ctgcgaaaac gctcgattac caaagcccgt tgcccaccac gcgctatacg ctgccggagc
     5461 tgttagacaa ttcccagcag ttgatccatg aagcgcggaa actgacgcct ccgcagatta
     5521 gcacgctgat gcgcatcagc gacaaactgg cgggtatcaa cgccgctcgc tttcatgact
     5581 ggcagccaga tttcacgccg gagaatgccc gccaggcgat tctggcattc aaaggtgatg
     5641 tctacaccgg cttgcaggcc gaaaccttca gcgaagacga tttcgatttt gcccaacagc
     5701 atttgcgaat gctttccggc ttgtatggcg tactgcgccc gctcgattta atgcagcctt
     5761 atcgtctgga aatggggatc cgtcttgaga atgcccgagg gaaagatctg tatcaattct
     5821 ggggagatat catcaccaac aagctgaacg aggcgctcgc agcacaaggc gataatgtgg
     5881 tgattaatct ggcgtcagat gagtatttta aatcggtgaa gccgaagaaa ttgaatgccg
     5941 agattatcaa gccggtgttc ctcgatgaga agaacggcaa atttaagatc atcagcttct
     6001 acgctaagaa agcacgcggt ctgatgagtc gtttcattat tgaaaatcgg ctgaccaaac
     6061 cagagcaact gactggtttt aatagcgaag gttacttctt tgatgaagct tcctccagca
     6121 acggcgaact ggtgtttaaa cgctacgagc agcgttaagt ttgcagtcaa tgccggatgg
     6181 caatccggca ttgatattta gcgatgatgt ttgtcaggcc ccggacgatg atcgccacga
     6241 tgatcattgt gcttgttatg gcgcggcgat ggcggcggtc catatgggtg ccagcgattg
     6301 cctcgccatt cataatgttg tttccaccag ccgtggtcgc gccagtgacc gccatcccag
     6361 taatagccac gattatcacg atcgcctatc tgtaatttta ctgacgggac taacgtcatt
     6421 tccgcagcct gcgcagccat gggagcgacc agaaccaggg aaagtgcgag tacgatagat
     6481 tgcatctttt tcacaggtta ctccttttat cattgcccgt tatgggtcga tatccggaga
     6541 ttacgcgatg agaacggcag ttgttattct ccgcaatcct aatctctaag ttaccgcatt
     6601 tattgagcat ttctcctttt tccctgtcgt ttttctccat aatttcttca taaaaaagcc
     6661 gggctgcata aaagcaaacc cggcctgatt gagataatga atagatatta ctgatgattc
     6721 atcatcaatt tacgcaacgc agcaaaatcg gcgggcagat tatgtgaaag caagggtaaa
     6781 tcggcacgtt ctgccagctc ttttggcaga tccaacgttt caccgagaat cgcttccacg
     6841 ctctctttaa atttcgccgg atgcgcggtg ccgaggaaca agccatattc gcctggatgc
     6901 aactggtcac gcagtgcacg atacgctacg gcagcgtgcg gctccgaagt gtagcccagt
     6961 tcttttaact cacgcattgt ctgttgcgtg gtttcatcat ccacggctgc ataacccagc
     7021 tctttcagtt gccagatttt gcggcggaac aactcttcca cacgcggcca gttgttcggc
     7081 tgactcacgt ccatcgcgtt ggataacgtc gcctgagtcg ctttgggtga ccactgaccg
     7141 tcgtgcagga aacgtggcac ggtatcgttc acgttggtcg cagcaataaa acgtttcacc
     7201 ggcagaccga gtgacttcgc cagcagaccc gccgtcaaat cgccgaagtt tccgcttggc
     7261 accgagacaa ccagctggtt gcgcgcctcc tgcggcagct gcgcaacagc ttcaaagtag
     7321 tagcaaatct gcgccagcaa acggctgatg ttaatcgagt tagctgagtt tagccccagc
     7381 gccactttca gttcttcatc atcaaacgcc tgcttcacca gcgcctgaca ggcatcgaaa
     7441 tcgccgtcga tggcaacagt ttcaatattg ccgcccaatg tacagaacag tttttcttgc
     7501 agtggactga ttttgcctcg tggatagagg ataaccactt tcacattcgg caaaccgtag
     7561 aaagcatgag ccactgccgc tccggtatca ccggaggtcg cggtcagaat ggtcactggc
     7621 ttatcgcccg caatatgggt cagcatttgt gccataaagc gaccgccgaa atctttaaat
     7681 gccagcgttg gcccgtggaa caattccaga caaccgacat cgctttcaac attggcgacc
     7741 ggagccggga agacaaacgc cgcgcgtacg cgctcttcca ggatttcctg cgggatttca
     7801 tcaccaataa acgccgagag gatcttcgca ctgcgggtga caaaatccag cttcagcatc
     7861 tcatcaattt cagtcaggct gaattccggc aggtcgtgcg ggaaaaacag cccctgattt
     7921 ttgcccaacc cctgggttac ggcttgcgca aagctgacct gctcattgtg atctttcaga
     7981 ttgtagagtt tcatttagtt ttccagtact cgtgcgcccg ccgtatccag ccggcaaata
     8041 tgaacaaaac cttcctgatt ttgcaggtag ttcttaccca accagtcggc aacgcgctgg
     8101 gcggtatccg gcttgtcaca cagagcgaac aaagtcgggc cggagccgga gataccgctc
     8161 gctaccgcgc cgatttccgc gaccgcctgc cgcgcctgcc ggaagcctgg cagtaaccgt
     8221 tcacggtagg gttcagcgat aacatctttc atcagcttcg cggcaagctc aggctgacgg
     8281 gaatagcagg cgtgaataaa gcctgccaga tgtcgcccgt gcgcaatgca atcctggcgg
     8341 cgatactgcg ccggtaaaat agccctggct tctgccgtcg agactttaat ccccggatac
     8401 gccagcaccc acagccactc atcaaaccct ggcacttgct ggctgatgat gtcgttttct
     8461 tcgatcatca actgcatacc accgagaaaa cacggtgcca cgttgtcgta atgaatgctg
     8521 ccggagatac gcccttccag ctcgcccatc aaagccagca aacgggtgtc attaagcggc
     8581 ttgccgcagt gttcattcat cgccatcagc gccgcgacca ctgagcaggc gctggagcct
     8641 aagcccgagc cgatcggcat attcttttcc agggtcatcg ccactggaat ttgcttaccc
     8701 agctcctggc aaaaacgctc ccaacactga taaacgatat tttcccgagg ttctgacggc
     8761 agcttatcgg caaagcgtcc gaggttattg agactgaatg tctctgccgc ctcaaccgtg
     8821 actacatctc cgagcaatgc accatcaacg ggtgtcaccg ccgccccgag cacatcgaac
     8881 ccgacgctca tattggcact ggaagccggg gcataaactt taaccatgtc agactcctaa
     8941 cttccatgag agggtacgta gcagatcggc aaagacaccg gcagctgtaa cgtcattgcc
     9001 cgcaccatat ccgcgcagca ccaacggcag cggctgataa tagtggctat agaaggccag
     9061 ggcgttttcg ccgtttttca ctttgaacag cggatcgttg ccatccactt cagcaatctt
     9121 cacgcggcag acgccatctt catcaatatt gccaacatag cgcaaaactt ttccttcatc
     9181 acgggccttc gccacacgag cggcaaagag atcatcgagc tgtgacagat tcgccataaa
     9241 agcggcaaca tcaccctcgg cgttaaactc agcgggtagc acaggttcaa tttcaatatc
     9301 cgccagctcc agttcacgtc ccgtttcacg agcgagaatc aatagtttac gcgccacatc
     9361 cataccagaa agatcatctc gcggatccgg ttcggtataa cccatttccc gcgccaacgt
     9421 ggtcgcctcg gagaaactca tgccttcgtc taacttgccg aagatataag aaagcgaacc
     9481 agaaagaatg ccggagaact tcatcaattc atcacctgca ttgagcagat tttgcaggtt
     9541 ctcaataacc ggtaatccag ccccaacgtt ggtgtcatag aggaatttac gccgcgattt
     9601 ttccgccgca tgacgcaaca gatggtagta atccatcgac gaggtgttag cctttttgtt
     9661 cggcgtgacg acgtggaaac cttcgcgcag gaagtcggca tattgatccg ccactgcctg
     9721 gctggaggtg cagtcaacaa tgaccgggtt cagcagatga tattctttca cgaggcgaat
     9781 taagcgcccg agattaaacg gctctttggc ttgcgccagt tcttcctgcc agttttccag
     9841 attaaggcca tgtacattgg tgagcagagc cttcgagttg gcaacgccgc agacacgtaa
     9901 gtcgatatgt ttattcttca accagctttg ctgacgcttc agttgctcca gcagcgcacc
     9961 gccaacgcca ccgacgccaa tcacaaacac ttcgataacc tgatcggtat tgaacagcat
    10021 ctgatgagta acgcgcacgc cagtggtcgc atcatcgtta tttaccacga cagagattga
    10081 gcgttcagaa gatccctgag caatggcgac aatgttgata ttggcgcggg ccagcgcggc
    10141 aaagaatttc gccgagatcc cacgcaaggt gcgcatacca tcacctacca ccgagataat
    10201 ggccagccgt tccgtcaccg ccagcggctc cagtaagcct tctttcagtt ccaggtagaa
    10261 ctcttcctgc attgcccgtt cagctcgcac acagtcgctt tgtggaacgc agaaactgat
    10321 actgtattcg gaagatgatt gcgtaatcag caccacggaa atacgggcgc gtgacatcgc
    10381 tgcaaagacg cgcgccgcca tgccgaccat ccctttcatc cccggaccag aaacgctgaa
    10441 cattgccatg ttattcagat tggaaatgcc cttgaccggt aattcgtctt catcacggct
    10501 ggcaccaatg agcgtacctg gtgcttgagg atttccggta tttttaatca ggcaagggat
    10561 ctggaactgg gcgatggggg taattgtgcg ggggtgaaga actttagcgc cgaagtagga
    10621 aagctccatc gcttcctggt aggacatcga cttcaacaac ctcgcatcgg gcacctgacg
    10681 cgggtcgcag gtatagaccc cgtcaacgtc cgtccaaatc tcgcaacaat cggcgcgtaa
    10741 acaggcagcc agcaccgccg cggagtagtc ggaaccgtta cgtccaagca ccaccagttc
    10801 gcctttttca ttaccggcgg tgaaacctgc catcaacacc atgtgatcag ccggaatacg
    10861 acttgccgca atacggcggg tggactcagc aatatcgacg gtagattcga ggtaatgccc
    10921 cactgccagc agtttttcga ccggatcgat aacggtaacg ttgtgaccac gcgcttctaa
    10981 cacgccggcc ataatggcga tcgacatttt ctcgccacgg caaatcagcg cagcgttgat
    11041 gctatccggg cactgcccca acaaactaat gccatgcagg acatgtttta tttgggcaaa
    11101 ttcctggtcg acgaaagttt tcagttgcgc cagcgggaat cccggctgag cggcggcgag
    11161 tcccgtcaga agttcggcaa aaatacgttc ggcatcgctg atattgggta aagcatcctg
    11221 gccgctaatg gttttttcaa tcatcgccac caggtggttg gtgattttgg cgggggcaga
    11281 gaggacggtg gccacctgcc cctgcctggc attgctttcc agaatatcgg caacccgcag
    11341 aaaacgttct gcatttgcca ctgatgtacc gccgaacttc aacactcgca tggttgttac
    11401 ctcgttacct ttggtcgaaa aaaaagcccg cactgtcagg tgcgggcttt tttctgtgtt
    11461 tcctgtacgc gtcagcccgc accgttacct gtggtaatgg tgatggtggt ggtaatggtg
    11521 gtgctaatgc gtttcatgga tgttgtgtac tctgtaattt ttatctgtct gtgcgctatg
    11581 cctatattgg ttaaagtatt tagtgaccta agtcaataaa attttaatt
//
LOCUS       DAZFLD010000149        11443 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.150, whole genome shotgun sequence.
ACCESSION   DAZFLD010000149 DAZFLD010000000
VERSION     DAZFLD010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11443
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.150"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..430
                     /locus_tag="ACHKBL_003488"
     CDS             <1..430
                     /locus_tag="ACHKBL_003488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708149.1"
                     /note="catalyzes the formation of (1 R,6
                     R)-2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate
                     in menaquinone biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HFO5820308.1"
                     /translation="GHPGLQNAEQRTERQRSDRQWAQRFRTEPLTAVFADWYQQPVFA
                     SLNDEQRRELVALRSNNNGATLAAMLEATSLAVQPDLRANLSARTFAFYYLCGERDSK
                     FRALAAELAAECHVIPRAGHNAHRENPAGVIASLAQILRF"
     gene            445..1302
                     /gene="menB"
                     /locus_tag="ACHKBL_003489"
     CDS             445..1302
                     /gene="menB"
                     /locus_tag="ACHKBL_003489"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008935 - 1,4-dihydroxy-2-naphthoyl-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HFO5820309.1"
                     /translation="MIYPDEAMLYAPVEWHDCSEGFEDIRYEKSTDGIAKITINRPQV
                     RNAFRPLTVKEMIQALADARYDDNIGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSG
                     VHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
                     SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWC
                     REMLQNSPMALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQP
                     DFSKFKRNP"
     gene            1302..2264
                     /gene="menC"
                     /locus_tag="ACHKBL_003490"
     CDS             1302..2264
                     /gene="menC"
                     /locus_tag="ACHKBL_003490"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HFO5820310.1"
                     /translation="MRSAQVYRWQIPMDAGVVLRDRRLKTRDGLYVCLREGEREGWGE
                     ISPLPGFSQETWEEAQSGLLAWVNNWLAGDSELPQMPSVAFGVSCALAELADTLPQAA
                     NYRAAPLCNGDPDDLILKLADMPGEKVAKVKVGLYEAVRDGMVVNLLLEAIPDLHLRL
                     DANRAWTPLKGQQFAKYVNPDYRDRIAFLEEPCKTRDDSRAFARETGIAIAWDESLRE
                     PDFAFVAEEGVRAVVIKPTLTGSLEKVREQVQAAHALGLTAVISSSIESSLGLTQLAR
                     IAAWLTPDTIPGLDTLDLMQAQQVRRWPGSLLPLVDVDALERLL"
     gene            2261..3616
                     /gene="menE"
                     /locus_tag="ACHKBL_003491"
     CDS             2261..3616
                     /gene="menE"
                     /locus_tag="ACHKBL_003491"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008756 - o-succinylbenzoate-CoA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HFO5820311.1"
                     /translation="MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELASSF
                     AMQGVVEGSGVMLRAWNTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQF
                     ALVPDGENTFPALTSLHIQRVEGAHAATWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
                     ASAEGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGC
                     THASLVPTQLWRLLVNRSSVSLKAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFAS
                     TVCAKEADGLADVGSPLPGREVKIVNDEVWLRAASMVEGYWRNGQLVSLVNDEGWYAT
                     RDRGEMHNGKLTIVGRLDNLFFSGGEGIQPEEVERVIAAHPAVLQVFIVPVADKEFGH
                     RPVAVVEYDQQTVDLDEWVKDKLARFQQPVRWLNLPPELKNGGIKISRQALKEWVQRQ
                     Q"
     gene            3726..3992
                     /gene="pmrD"
                     /locus_tag="ACHKBL_003492"
     CDS             3726..3992
                     /gene="pmrD"
                     /locus_tag="ACHKBL_003492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311174.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein PmrD"
                     /protein_id="HFO5820312.1"
                     /translation="MEWLVKKSCCNKQDNRHVIMLCDAGGAIKMIAEVKSDFAVKVGD
                     LLSPLQNALYCINREKLHTVKVLSASCYSPDEWERQCKAAGKTQ"
     gene            complement(3986..4372)
                     /gene="arnF"
                     /locus_tag="ACHKBL_003493"
     CDS             complement(3986..4372)
                     /gene="arnF"
                     /locus_tag="ACHKBL_003493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnF"
                     /protein_id="HFO5820313.1"
                     /translation="MGLMWGLFSVIIASAAQLSLGFAASHLPPMTHLWDFIAALLAFG
                     LDARILLLGLLGYLLSVFCWYKTLHKLALSKAYALLSMSYVLVWIASMVLPGWEGTFS
                     LKALLGVACIMSGLMLIFLPTTKQRY"
     gene            complement(4372..4707)
                     /gene="arnE"
                     /locus_tag="ACHKBL_003494"
     CDS             complement(4372..4707)
                     /gene="arnE"
                     /locus_tag="ACHKBL_003494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnE"
                     /protein_id="HFO5820314.1"
                     /translation="MIWLTLVFASLLSVAGQLCQKQATCFVAINKRRKHIVLWLGLAL
                     ACLGLAMVLWLLVLQNVPVGIAYPMLSLNFVWVTLAAVKLWHEPVSPRHWCGVAFIIG
                     GIVILGSTV"
     gene            complement(4704..6356)
                     /gene="arnT"
                     /locus_tag="ACHKBL_003495"
     CDS             complement(4704..6356)
                     /gene="arnT"
                     /locus_tag="ACHKBL_003495"
                     /EC_number="2.4.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A)
                     4-amino-4-deoxy-L-arabinosyltransferase"
                     /protein_id="HFO5820315.1"
                     /translation="MKSVRYLIGLFAFIACYYLLPISTRLLWQPDETRYAEISREMLA
                     SGDWIVPHLLGLRYFEKPIAGYWINSIGQWLFGANNFGVRAGVIFATLLTAALVTWFT
                     LRLWRDKRLALLAAVIYLSLFIVYAIGTYAVLDPFIAFWLVAGMCSFWLAMQAQTWKG
                     KSAGFLLLGITCGMGVMTKGFLALAVPVLSVLPWVATQKRWKDLFIYGWLAVISCVLT
                     VLPWGLAIAQREPDFWHYFFWVEHIQRFAMDDAQHRAPFWYYLPVIIAGSLPWLGLLP
                     GALYAGWKNRKHSATVYLLSWTIMPLLFFSVAKGKLPTYILSCFAPLAMLMAHYALLA
                     AKNNPLALRINGWINIAFGVTGIIATFVVSPWGAMNTPVWQTFESYKVFCAWSIFSLW
                     AFFGWYTLTNVEKTWSFAALCPLGLALLVGFSIPDRVMEGKHPQFFVEMTQESLQPSR
                     YILTDSVGVAAGLAWSLQRDDIIMYRQTGELKYGLNYPDAKGRFVSGDEFANWLNQHR
                     QEGIITLVLSVDRNEDINSLAIPPADAIDRQERLVLIQYRPK"
     gene            complement(6356..7246)
                     /gene="arnD"
                     /locus_tag="ACHKBL_003496"
     CDS             complement(6356..7246)
                     /gene="arnD"
                     /locus_tag="ACHKBL_003496"
                     /EC_number="3.5.1.n3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-deoxy-4-formamido-L-arabinose-
                     phosphoundecaprenol deformylase"
                     /protein_id="HFO5820316.1"
                     /translation="MTKVGLRIDVDTFRGTREGVPRLLEILSKHNIQASIFFSVGPDN
                     MGRHLWRLVKPQFLWKMLRSNAASLYGWDILLAGTAWPGKEIGHANADIIREAAKHHE
                     VGLHAWDHHAWQAHSGNWDRQTMIDDIARGLRTLEEIIGQPVTCSAAAGWRADQQVIE
                     AKEAFHLRYNSDCRGAMPFRPLLESGNPGTAQIPVTLPTWDEVIGRDVKAEDFNGWLL
                     NRILRDKGTPVYTIHAEVEGCAYQHNFVDLLKRAAQEGVTFCPLSELLSGMLPLGQVV
                     RGNIAGREGWLGCQQIAGSR"
     gene            complement(7243..9225)
                     /gene="arnA"
                     /locus_tag="ACHKBL_003497"
     CDS             complement(7243..9225)
                     /gene="arnA"
                     /locus_tag="ACHKBL_003497"
                     /EC_number="1.1.1.305"
                     /EC_number="2.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-amino-4-deoxy-L-arabinose
                     formyltransferase/UDP-glucuronic acid oxidase ArnA"
                     /protein_id="HFO5820317.1"
                     /translation="MKTVVFAYHDMGCLGIEALLAAGYEISAIFTHTDNPGEKAFYGS
                     VARLAAERGIPVYAPDNVNHPLWVERIAQLSPEVIFSFYYRHLICDEILQLAPAGAFN
                     LHGSLLPKYRGRAPLNWVLVNGETETGVTLHRMVKRADAGAIVAQLRIAIAPDDIAIT
                     LHHKLCHAARQLLEQTLPTIKHGNILEIAQRENEATCFGRRTPDDSFLEWHKPASVLH
                     NMVRAVADPWPGAFSYVGNQKFTVWSSRVHPHASKAQPGSVISVAPLLIACGDGALEI
                     VTGQAGDGITMQGSQLAQTLGLVQGSRLNSQPACAARRRTRVLILGVNGFIGNHLTER
                     LLREDHYEVYGLDIGSDAISRFLNHPHFHFVEGDISIHSEWIEYHVKKCDVVLPLVAI
                     ATPIEYTRNPLRVFELDFEENLRIIRYCVKYRKRIIFPSTSEVYGMCSDKYFDEDHSN
                     LTVGPVNKPRWIYSVSKQLLDRVIWAYGEKEGLQFTLFRPFNWMGPRLDNLNAARIGS
                     SRAITQLILNLVEGSPIKLIDGGKQKRCFTDIRDGIEALYRIIENAGNRCDGEIINIG
                     NPENEASIEELGEMLLASFEKHPLRHHFPPFAGFRVVESSSYYGKGYQDVEHRKPSIR
                     NARRCLDWEPKIDMQETIDETLDFFLRTVDLTDKPS"
     gene            complement(9225..10193)
                     /gene="arnC"
                     /locus_tag="ACHKBL_003498"
     CDS             complement(9225..10193)
                     /gene="arnC"
                     /locus_tag="ACHKBL_003498"
                     /EC_number="2.4.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate
                     4-deoxy-4-formamido-L-arabinose transferase"
                     /protein_id="HFO5820318.1"
                     /translation="MFEIHPVKKVSVVIPVYNEQESLPELIRRTTAACESLGKEYEIL
                     LIDDGSSDNSAHMLVEASQAENSHIVSILLNRNYGQHSAIMAGFSHVTGDLIITLDAD
                     LQNPPEEIPRLVAKADEGYDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYG
                     CMLRAYRRHIVDAMLHCHERSTFIPILANIFARRAIEIPVHHAEREFGESKYSFMRLI
                     NLMYDLVTCLTTTPLRMLSLLGSIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFAV
                     LFTFIGAQFIGMGLLGEYIGRIYTDVRARPRYFVQQVIRPSSKENE"
     gene            complement(10197..11336)
                     /gene="arnB"
                     /locus_tag="ACHKBL_003499"
     CDS             complement(10197..11336)
                     /gene="arnB"
                     /locus_tag="ACHKBL_003499"
                     /EC_number="2.6.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024188371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4-deoxy-L-arabinose
                     aminotransferase"
                     /protein_id="HFO5820319.1"
                     /translation="MSEFLPFSRPAMGVEELAAVKEVLESGWITTGPKNQALEQAFCQ
                     LTGNQHAIAVSSATAGMHITLMALEIGKGDEVITPSLTWVSTLNMISLLGATPVMVDV
                     DRDTLMVTPEAIESAITPRTKAIIPVHYAGAPADIDAIRAIGERYGIAVIEDAAHAVG
                     TYYKGRHIGAKSTAIFSFHAIKNITCAEGGLIVTDNENLARQLRMLKFHGLGVDAYDR
                     HTWGRAPQAEVLTPGYKYNLTDINAAIALTQLVKLEHLNTRRREIAQQYQQALAALPF
                     QPLSLPAWSHVHAWHLFIIRVDEQRCGISRDALMEALKERGIGTGLHFRAAHTQKYYR
                     ERFPTLSLPNTEWNSERICSLPLFPDMTTADADRVITALQQLAGQ"
BASE COUNT         2789 a         2972 c         3071 g         2611 t
ORIGIN      
        1 cgggcatcct gggttgcaaa atgctgaaca acgcacggaa cgtcagcgtt ccgatcgcca
       61 atgggcgcag cgttttcgca cagaaccgtt aacggcggta tttgctgact ggtatcaaca
      121 gcctgttttt gcctcactca atgacgaaca acgccgggag ctggttgcgc tgcgcagcaa
      181 caataatggc gcaacacttg ccgccatgct ggaggcgact tctctcgccg tccagcccga
      241 tttacgtgct aaccttagcg cccgcacatt tgcgttttat tatttatgtg gtgaacgtga
      301 cagcaaattc cgcgccctgg cggcggaact ggctgccgaa tgccatgtca ttcctcgcgc
      361 cggacataac gcgcaccggg aaaatcccgc tggcgtaatc gcaagtctgg cgcagatctt
      421 gcgtttctga ctaaaggaca caatatgatt tatcctgatg aagcaatgct ttacgcaccg
      481 gttgaatggc acgactgctc cgaaggtttt gaggacattc gttatgaaaa atccaccgac
      541 ggtatcgcaa aaatcaccat taatcgtccg caggtgcgca atgccttccg tcctctgacg
      601 gtaaaagaga tgatccaggc actggcagat gcgcgttatg acgacaacat cggcgtgatc
      661 attctgactg gcgcaggcga taaagcgttc tgctccggcg gtgaccagaa agtgcgtggt
      721 gattacggcg gctataagga tgattccggc gtacatcacc tgaacgtgct ggacttccag
      781 cgtcagatcc gtacctgccc gaaaccggtt gtcgcgatgg tggctggcta ctccattggt
      841 ggcggtcacg ttctgcacat gatgtgcgac ctgactatcg cggcagataa tgccatcttc
      901 ggtcagactg gcccgaaagt cggttccttc gacggcggct ggggcgcttc ctacatggcg
      961 cgcatcgtcg ggcagaaaaa agcgcgtgaa atctggttcc tgtgccgtca gtacgacgcg
     1021 aaacaggcgc tggatatggg ccttgtaaat accgtggtgc cgctggcgga tctggaaaaa
     1081 gaaaccgtgc gctggtgccg tgaaatgctg caaaacagcc cgatggcgct gcgctgcctg
     1141 aaagctgcac tgaacgccga ctgtgacggt caggcgggtc tgcaggagct ggcgggcaac
     1201 gccaccatgc tgttctacat gacggaagaa ggtcaggaag gtcgcaacgc tttcaaccag
     1261 aaacgtcagc ctgatttcag caaatttaaa cggaatccgt aatgcgtagc gcgcaggtat
     1321 accgctggca gatccccatg gacgcggggg tggttctgcg cgacaggcgg ttaaaaaccc
     1381 gcgacgggct gtatgtttgc ctgcgtgagg gcgagcgcga agggtggggg gagatctccc
     1441 cactaccggg cttcagccag gaaacctggg aagaggcaca aagcgggctg cttgcctggg
     1501 taaataactg gctggcaggt gatagcgagc taccgcaaat gccttccgtc gcctttggcg
     1561 taagctgtgc attggcggag ctggcagaca cgctcccgca ggcggccaat tatcgtgcgg
     1621 caccactgtg taatggcgat ccggacgatc tgatcctgaa acttgccgat atgccgggcg
     1681 agaaagtggc gaaggtcaaa gtgggattgt acgaagccgt gcgcgacggc atggtggtca
     1741 atctgttgct ggaggcaatt ccggatctgc atttgcgtct tgacgcgaac cgcgcctgga
     1801 cgccgctgaa aggccagcag tttgccaaat acgttaaccc ggattatcgc gaccgcatcg
     1861 cgtttctcga agagccgtgc aaaacccgcg atgattcccg tgcgtttgcc cgtgaaaccg
     1921 gcattgccat tgcctgggat gaaagcctgc gcgagccgga ttttgccttt gtggctgaag
     1981 agggcgtgcg cgcggtagtt atcaaaccca cgctcacggg cagcctggag aaagtacgcg
     2041 agcaagtgca ggcggcgcat gcgctggggc tgacggcggt gatcagttct tccattgagt
     2101 cgagcttagg tctaacgcaa ctggcgcgga ttgctgcctg gttaacgcca gacaccattc
     2161 cgggactgga cacgctggat ctgatgcagg cgcagcaggt tcgtcgctgg ccggggagcc
     2221 tgctgccgtt agtggacgtt gatgcactgg agcggctgtt atgatcttct ctgactggcc
     2281 gtggcgtcac tggcggcaag tgcggggaga agccatcgcc ttacgtctta atgacgagca
     2341 actcaactgg cgcgagcttt gtgctcgcgt cgatgaatta gcctccagct ttgcgatgca
     2401 gggtgtggtt gagggcagcg gcgtgatgtt gcgggcgtgg aatacgccgc aaacgctgct
     2461 ggcctggctg gcgttactgc aatgcggggc gcgggtgctg cccgtgaatc cacagctgcc
     2521 gcaaccgtta cttgaggaat tgctccccaa tcttacgctg caatttgcgc tggtgccgga
     2581 tggggaaaac acgtttccgg cattaacgtc gctgcacatt cagcgggttg aaggcgcaca
     2641 tgccgctacg tggcagccga cgcgtctgtg ctcaatgacg ttgacctcgg gttctaccgg
     2701 gctgccgaaa gccgctgtcc atacttatca ggcccatctt gccagtgccg aaggggtgtt
     2761 atcgttgatt ccgtttggcg atcacgatga ctggttgctc tctttaccgc tgtttcacgt
     2821 ctccggtcag ggaattatgt ggcgctggtt atacgctggt gcgcggatga cggtgcgtga
     2881 taaacagcca ttggagcaaa tgctggcagg ctgtactcac gcttcactgg tgccaacaca
     2941 actctggcgt ttgctggtta accgcagttc cgtttccctg aaagcggtgt tacttggcgg
     3001 cgcggctatc ccggtcgagt tgacggaaca ggcgcgcgag caggggattc gttgcttttg
     3061 cggctatggt ctgaccgagt ttgcctccac ggtgtgtgcg aaagaagccg acggtctggc
     3121 agacgttggt tcgccgctgc cgggtcggga agtgaaaatc gttaatgatg aagtgtggct
     3181 gcgggctgct agtatggtag aaggttactg gcgtaacggg caactggttt cactggttaa
     3241 tgacgaaggc tggtacgcta cgcgcgatcg cggtgagatg cataatggca agctgaccat
     3301 tgtcggacgt ttagacaatc tattcttcag tggcggagag ggtattcagc cggaagaagt
     3361 cgagcgcgta attgctgcac atcctgcggt tttgcaggtg tttatcgtcc ccgttgccga
     3421 caaggagttt ggtcatcgac cggtggcggt ggtggagtat gaccagcaaa ccgttgatct
     3481 tgacgaatgg gtgaaagaca agttggctcg ttttcaacaa ccggtgcgct ggctgaatct
     3541 gccgccggag ctgaaaaacg gcggtattaa aatttcacgt caggcgctaa aagagtgggt
     3601 gcaacgccag caataattaa ccctccgttt cacttctcat ttctgccatt ccattgccgt
     3661 tgataaagag tttatctgtt aatctgtaaa ttgatgtgaa aactcttagc aaacaggata
     3721 atgcaatgga atggctggtc aaaaaatcgt gctgcaataa acaagataac agacatgtaa
     3781 ttatgctctg cgatgctggc ggcgcgataa aaatgatcgc cgaagtgaag agtgacttcg
     3841 ccgtgaaagt gggggattta ctctcgcctt tgcagaatgc gctttactgt attaatcgtg
     3901 aaaagctgca cacggtgaaa gtcctttctg caagctgtta tagccccgat gagtgggaac
     3961 ggcagtgtaa agcggcaggg aaaactcagt aacgttgttt tgttgtgggc aggaaaatca
     4021 gcatcaaccc gctcataata caggctactc ccagtagtgc tttcagcgaa aaagtccctt
     4081 cccagccggg aagtaccatt gaagcaatcc ataccagcac ataactcata ctgagcaagg
     4141 cataggcttt gcttagtgca agtttatgta gtgttttata ccaacaaaaa acggaaagca
     4201 gataacccag cagcccgagt aacagtatcc gggcatccag gccaaaagct aacaacgccg
     4261 cgataaaatc ccataagtgc gtcatcggcg gcagatgact cgccgcaaaa cccaggctta
     4321 attgcgcagc ggaggcgata atgacgctga ataatcccca catcaggccc attacacggt
     4381 actcccgagg atcacaatgc caccaataat gaacgccacc ccacaccagt gacgcggcga
     4441 taccggttcg tgccacagtt ttactgcagc cagcgtcacc cagacaaaat tcagacttaa
     4501 catcgggtag gcaatgccta ccggtacgtt ctgcaagacc agcagccaga gcaccatggc
     4561 aagaccaaga caagccagcg ccagtcccag ccacagcacg atatgtttgc gccgtttgtt
     4621 tatcgccaca aagcaggttg cctgtttctg acacaactgc ccggcaacgc taagcaagct
     4681 ggcaaagact aatgttagcc agatcatttg ggacgatact gaatcagcac cagacgctcc
     4741 tgacgatcga tggcatctgc gggcggaatg gcgagactgt tgatatcttc attgcggtca
     4801 accgaaagca cgagtgtaat aatcccctcc tgacgatgtt gattaagcca gtttgcgaac
     4861 tcatcaccgc tgacaaatct ccctttcgca tccggataat taaggccgta tttcaactca
     4921 cctgtctggc gatacatgat gatgtcatcg cgttgcaggc tccatgccag acctgcggca
     4981 acaccgacgc tatcagtaag aatatagcgg cttggctgaa gtgattcttg tgtcatctcg
     5041 acaaaaaatt gcggatgttt tccttccatg actctgtcag gaattgaaaa tcctaccagc
     5101 aacgccagcc ccagcgggca aagcgcggca aaagaccagg tcttttcgac gtttgttaag
     5161 gtgtaccagc cgaaaaatgc ccatagcgaa aaaatcgacc aggcacaaaa gactttatag
     5221 ctctcgaagg tttgccacac cggcgtgttc attgctcccc agggggagac cacaaatgtg
     5281 gcaataatgc cagtgacgcc aaaagcgatg ttaatccagc cattaatccg caacgccaga
     5341 ggattatttt ttgctgccag caaagcgtaa tgcgccatca gcattgccag aggagcaaag
     5401 caagaaagaa tataggtggg caatttgcct ttggcgacgg agaaaaacaa cagcggcatt
     5461 atcgtccagc tcaacaaata gacggttgcg gaatgcttgc ggtttttcca gcctgcgtac
     5521 agtgctccgg ggagtaatcc cagccacggc aggctaccgg caatgatgac cggcaagtag
     5581 taccagaacg gagctctatg ttgggcatca tccattgcaa agcgttgaat atgctcaacc
     5641 cagaaaaaat agtgccagaa gtcaggctcc cgctgcgcta tcgccagtcc ccaggggaga
     5701 accgtcagta cgcaactgat gaccgccagc cagccgtaaa taaagagatc tttccagcgt
     5761 ttttgcgttg caacccatgg aagcacgctt aataccggca cggcaagggc gagaaaacct
     5821 ttggtcatca cccccatgcc gcaagtgatt cccagcagta aaaatcccgc gcttttgcct
     5881 ttccacgtct gtgcctgcat tgccagccag aagctgcaca ttcccgccac cagccagaag
     5941 gcaataaacg gatcgagcac ggcataagtg ccaatggcat agacaataaa caatgagaga
     6001 taaatcactg cggcgagtag agccagacgt ttatcgcgcc ataagcgcag agtaaaccag
     6061 gtcaccagcg cggcagttaa cagggtcgca aagataacgc ctgcccgcac accaaagtta
     6121 ttcgcgccaa atagccattg cccaatgctg ttaatccagt atccggcaat gggtttttcg
     6181 aaataacgta gccctaacag atggggcaca atccagtcgc cggatgccag catttctcga
     6241 ctgatttccg cataacgcgt ttcatcgggt tgccatagca gacgcgtgct gatcggtaac
     6301 aggtaatagc aggcaataaa cgcgaagagg ccgataaggt aacgtaccga tttcatcagc
     6361 gactacccgc aatttgttgg caacccagcc agccttcacg tccggcaata tttccgcgaa
     6421 caacttgtcc gagcggcaac atccctgata acagttcgct taaagggcaa aatgtcacgc
     6481 cttcctgggc tgcccgttta aggagatcca caaaattatg ctgataagcg cagccttcaa
     6541 cttctgcatg aatggtataa accggcgtgc ctttatctcg caggatgcga ttgagtaacc
     6601 aaccgttaaa atcttctgct ttcacatccc gaccaatcac ttcatcccag gtgggtaagg
     6661 tcaccggaat ttgcgcagta ccagggtttc ctgattcgag cagtggacga aacggcatcg
     6721 ccccacgaca atcgctgttg tagcgcaaat ggaacgcttc ttttgcttcg atcacctgct
     6781 ggtcggcacg ccagccagcg gctgcggagc aggttaccgg ttggccgata atttcttcca
     6841 gggtgcgaag gccgcgtgcg atatcgtcga tcattgtttg ccgatcccaa ttaccgctat
     6901 gggcttgcca ggcatggtga tcccaggcgt gcaggccgac ttcgtgatgt ttagccgctt
     6961 cacgaatgat atcggcattg gcatgaccaa tctctttacc cggccaggcc gtacctgcca
     7021 gtaaaatatc ccagccataa agcgatgccg cgtttgagcg cagcatcttc cacaaaaact
     7081 gtggcttcac cagtcgccag agatggcggc ccatattgtc cgggccgacg ctgaaaaaaa
     7141 tgctggcctg aatattatgc ttactcaaga tttccagcag acgcggcacg ccttcacggg
     7201 tgccacgaaa ggtatcgaca tcaatgcgta agcctacttt ggtcatgatg gtttatccgt
     7261 aagatcaacg gtgcgcagga agaaatccag cgtttcgtcg atggtttcct gcatatcaat
     7321 tttcggttcc cagtccaggc agcggcgggc attgcggatg ctcggtttac gatgctctac
     7381 gtcctgatat cctttgccat agtagctgct actttcgaca acacgaaagc ctgcaaacgg
     7441 cgggaagtga tggcgcagcg gatgtttttc gaagctcgcc agcagcatct cgcccagttc
     7501 ctcaatgctc gcttcgttct caggattgcc aatgttgata atttcgccgt cgcagcgatt
     7561 tcccgcattt tcgataatgc ggtacaacgc ctcgatacca tcccggatat cagtaaagca
     7621 gcgtttttgt tttccgccat caattagctt aattggtgaa ccttctacca gattgagaat
     7681 gagttgcgta atagcgcggg agctgccaat tcgcgctgca ttaaggttat ccagtcgtgg
     7741 tcccatccag ttaaacggcc ggaagagggt gaactgtaaa ccctcttttt cgccataggc
     7801 ccagatcacc cgatcaagta attgttttga caccgaataa atccagcgtg gtttattcac
     7861 cgggccgacg gttaaattag aatggtcctc atcgaagtat ttatcgctac acatcccata
     7921 aacttctgaa gttgacggga agatgattcg cttacggtac ttcacgcagt agcggataat
     7981 gcgcagattc tcttcaaaat cgagttcaaa tacgcgcagc gggttgcggg tatattcaat
     8041 cggcgtggct atcgccacca gcggcaagac gacatcacat tttttgacgt gatactcaat
     8101 ccattcggaa tgaatactga tatcgccttc gacaaagtga aaatgcggat gattcagaaa
     8161 acggcttatc gcatcgctgc caatatccag accgtaaact tcataatgat cttcgcgcag
     8221 caggcgttct gtcagatggt tgccaataaa gccattcacc ccgaggatga gtacccgggt
     8281 acggcgtcgg gcggcacagg caggctggct attcaagcgt gaaccttgca ccaggcccag
     8341 cgtctgcgct aattgcgagc cctgcatagt aatgccgtcg cccgcctgtc cggtgacgat
     8401 ttccagcgcg ccatcgccac aggcaatcag cagtggcgct acagaaatca cgctccccgg
     8461 ctgtgctttg ctggcatgag gatgaacacg cgacgaccag acggtgaatt tctgattgcc
     8521 aacatagctg aaggcacccg gccacggatc ggcaacggca cgtaccatgt tgtgcagtac
     8581 ggatgccggc ttatgccatt cgaggaaact gtcatccggc gttctgcgac caaaacaggt
     8641 agcttcgttt tcgcgctggg cgatttccag aatattaccg tgtttaatgg tgggtaatgt
     8701 ctgctccagt agctggcgcg cggcatgaca taacttatga tgcaacgtaa tggcgatatc
     8761 gtctggcgca atggcaatgc gcagttgcgc cacaatggcc ccggcatcgg cacgtttcac
     8821 cattcggtgc aatgtaacgc cagtttccgt ttcaccgttg accagcaccc agttcagcgg
     8881 cgcgcgacca cgatattttg gtaacagcga accatgcaga ttaaatgcac ctgcgggagc
     8941 gagctgcaaa atctcgtcgc aaataagatg gcgataataa aaagagaaaa tcacctctgg
     9001 tgagagttgg gcaatacgtt ccacccacag cggatgatta acgttatctg gcgcataaac
     9061 cggaatgcct ctttccgccg ccagacgagc taccgaacca taaaaggctt tttcaccggg
     9121 attatcggta tgagtaaaaa tagcgctaat ttcgtaaccg gcagccagca gggcttcaat
     9181 accgaggcat cccatatcgt ggtaggcaaa aacgacggtt ttcattattc attttccttg
     9241 ctggatggac ggataacttg ctgaacaaaa tagcgggggc gggcgcggac atcggtgtag
     9301 atcctgccga tatattcacc gagtaatccc atgccgataa actgagcgcc aataaaagta
     9361 aacagcacgg caaatagcat aaagacgcct tctgccgccc attgtggtcc gaaggtcagg
     9421 cgtaaaatca ccagcagcac cgcaatgcta aaacctccaa tcgcaataat gctgccgagc
     9481 agactcagca tacgtagcgg cgttgtggta aggcaggtca ccaggtcgta catcaaatta
     9541 atcaggcgca taaaactgta tttggattca ccaaactcac gctcggcatg gtgcactgga
     9601 atttcaatgg cacggcgggc gaagatattc gccagaatcg ggataaaggt actgcgttca
     9661 tggcagtgca acatcgcatc gacaatatga cggcgatagg cgcgcagcat acaaccgtag
     9721 tcacccatcg ctttgccggt ggtgcgctga ataagccggt taatcatctt cgaagcggtt
     9781 ttacgaaacc agctgtcctg gcggttctgg cgtacagtcc ctaccacgtc gtaaccttca
     9841 tcggcttttg ccaccagacg ggggatttcc tctggcggat tctggagatc ggcatcgagg
     9901 gtaataatta agtcgccagt aacgtgactg aatcccgcca taatcgctga atgttgcccg
     9961 taattgcggt taagcaaaat agacacaata tggctgttct ccgcttgtga ggcttcgacc
    10021 agcatatgcg cggaattatc gctactgccg tcatcaatca gcaggatttc atactctttc
    10081 cccagtgatt cacaggctgc ggtggtgcgc ctgattaatt ccggtaagct ttcctgctcg
    10141 ttataaacgg gaataaccac cgagactttc ttaacagggt ggatttcaaa catggcttat
    10201 tgtcctgcga gttgctgaag ggctgtgatg acgcggtcgg catcggcggt ggtcatatcc
    10261 gggaacagcg gcaacgaaca gatgcgttcg ctattccatt cggtattcgg taacgacagc
    10321 gtgggaaaac gctcgcgata atatttttgt gtgtgagcgg cgcggaaatg taacccggta
    10381 ccaatgcctc tttcttttaa cgcttccatc aacgcatcgc gactgatacc acaacgttgt
    10441 tcatcgacac gaataataaa cagatgccag gcgtgaacgt gtgaccaggc gggaaggctt
    10501 aatggctgaa aggggagagc tgccagtgct tgctgatatt gctgggcaat ttcgcgccga
    10561 cgggtgttga ggtgctctaa tttgactaac tgtgtcaggg caatcgcggc gttaatatcg
    10621 gtcagattgt acttatagcc cggcgttaag acttcagcct gcggtgcacg gccccaggta
    10681 tgtctgtcat aggcatcgac gcccagaccg tgaaatttta gcatccgtag ctggcgggca
    10741 aggttttcat tatctgtaac aatcaggcca ccttccgcac aggtaatatt tttgatggcg
    10801 tgaaacgaaa aaatagcggt actttttgcg ccaatatgtc gccctttgta atacgtacct
    10861 acggcatggg cagcatcttc gataactgcg atgccgtaac gttcgccaat ggcgcgaatg
    10921 gcgtcaatat ctgctggcgc accggcataa tgcaccggaa tgatggcttt agtgcgtggc
    10981 gtaatggctg actcgatcgc ttcaggcgtg accatcagcg tatcgcggtc gacatccacc
    11041 attaccggcg ttgcacccaa caaggagatc atattgaggg ttgaaaccca ggtcagggaa
    11101 ggcgtaatca cttcatcgcc cttgccaatt tccaacgcca ttagcgtgat atgcattccg
    11161 gcggtggctg aactgaccgc gatggcatgc tgatttcccg tcaactggca aaaggcttgt
    11221 tcaagcgcct gattcttcgg accggttgtg atccaaccgg attcgagaac ctctttaact
    11281 gctgcgagtt cctccacgcc cattgctggt cgcgaaaaag gcaaaaattc tgacattgct
    11341 tttccttccg ccattgaata cctgtccact tatatttgct ataaagagtg ttgtgtatat
    11401 tttgccattt ggagcgaaat tctaaggata gaatattaac tta
//
LOCUS       DAZFLD010000150        11428 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.151, whole genome shotgun sequence.
ACCESSION   DAZFLD010000150 DAZFLD010000000
VERSION     DAZFLD010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11428
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.151"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            20..835
                     /gene="gspC"
                     /locus_tag="ACHKBL_003500"
     CDS             20..835
                     /gene="gspC"
                     /locus_tag="ACHKBL_003500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspC"
                     /protein_id="HFO5820320.1"
                     /translation="MPTLRFPFHLANHNKDAAINILIIFISIGLIIFNVNYFHTTIVK
                     NGQIINQPTNAFQSEFSLAALWRNENHAGVKDANPVAVNQETPKLSIALNGIVLTSND
                     ETSFVLINEGSEQKRYSLNEALESAPGTFIRKINKTSVVFETHGHYEKVTLHPGLPDI
                     IKQPDSESQNVLADYIIATPIRDGEQIYGLRLNPRKGLNAFTTSLLQPGDIALRINNL
                     SLTHPDEVSQALSLLLTQQSAQFTIRRNGVPRLINVSVAELTGMNGQRHERTQ"
     gene            819..2771
                     /gene="gspD"
                     /locus_tag="ACHKBL_003501"
     CDS             819..2771
                     /gene="gspD"
                     /locus_tag="ACHKBL_003501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system secretin GspD"
                     /protein_id="HFO5820321.1"
                     /translation="MKGLNKITCCLLAALLMPCAGHAENEQYGANFNNADIRQFVEIV
                     GQHLGKTILIDPSVQGTISVRSNDTFSQQEYYQFFLSILDLYGYSVITLDNGFLKVVR
                     SANVKTSPGMIADSSRPGVGDELVTRIVPLENVPARDLAPLLRQMMDAGSVGNVVHYE
                     PSNVLILTGRASTINKLIEVIKRVDVIGTEKQQIIHLEYASAEDLAEILNQLISESHG
                     KSQMPALLSAKIVADKRTNSLIISGPEKARQRITSLLKSLDVEESEEGNTRVYYLKYA
                     KATNLVEVLTGVSEKLKDEKGNERKPSSSGAMDNVAITADEQTNSLVITADQSVQEKL
                     ATVIARLDIRRAQVLVEAIIVEVQDGNGLNLGVQWANKNVGAQQFTNTGLPIFNAAQG
                     VADYKKNGGITSANPAWDMFSAYNGMAAGFFNGDWGVLLTALASNNKNDILATPSIVT
                     LDNKLASFNVGQDVPVLSGSQTTSGDNVFNTVERKTVGTKLKVTPQVNEGDAVLLEIE
                     QEVSSVDSSSNSTLGPTFNTRTIQNAVLVKTGETVVLGGLLDDFSKEQVSKVPLLGDI
                     PLVGQLFRYTSTERAKRNLMVFIRPTIIRDDDVYRSLSKEKYTRYRQEQQQRIDGKSK
                     ALVGSEDLPVLDENTFNSHTPAPSAR"
     gene            2781..4262
                     /gene="gspE"
                     /locus_tag="ACHKBL_003502"
     CDS             2781..4262
                     /gene="gspE"
                     /locus_tag="ACHKBL_003502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
                     /protein_id="HFO5820322.1"
                     /translation="MRIHSPYPASWALAQRIGYLYSEGEIIYLADTPFERLLDIQRQV
                     GQCQTMTSLSQADFEARLEAVFHQNTGESQQIAQDIDQSVDLLSLSEEMPANEDLLNE
                     DSAAPVIRLINAILSEAIKETASDIHIETYEKTMSIRFRIDGVLRTILQPNKKLAALL
                     ISRIKVMARLDIAEKRIPQDGRISLRIGRRNIDVRVSTLPSIYGERAVLRLLDKNSLQ
                     LSLNNLGMTAADKQDLENLIQLPHGIILVTGPTGSGKSTTLYAILSALNTPGRNILTV
                     EDPVEYELEGIGQTQVNTRVDMSFARGLRAILRQDPDVVMVGEIRDTETAQIAVQASL
                     TGHLVLSTLHTNSASGAVTRLRDMGVESFLLSSSLAGIIAQRLVRRLCPQCRQFTPVS
                     PQQAQMFKYHQLAVTTIGTPVGCPHCHQSGYQGRMAIHEMMVVTPELRAAIHENVDEQ
                     ALERLVRQQHNALIKNGLQKVISGDTSWDEVMRVASATLENEA"
     gene            4259..5455
                     /gene="gspF"
                     /locus_tag="ACHKBL_003503"
     CDS             4259..5455
                     /gene="gspF"
                     /locus_tag="ACHKBL_003503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system inner membrane protein
                     GspF"
                     /protein_id="HFO5820323.1"
                     /translation="MNYRYRAMTQDGQKLQGIIDANDERQARLRLREEGLFLLDIRPQ
                     KSSGVKTRRPRISHSELTLFTRQLATLSAAALPLEESLAVIGQQSSNKRLADVLNQVR
                     SAILEGHPLSDALQHFPTLFDSLYRTLVKAGEKSGLLAPVLEKLADYNENRQKIRSKL
                     IQSLIYPCMLTAVAIVVVIILLTAVVPKITEQFVHMKQQLPLSTRILLGLSDTLQHTG
                     PTLLATVFIVAVGFWLWLKRGNNRHRFHAMLLRVALIGPLICAINSARYLRTLSILQS
                     SGVPLLDGMNLSTESLSNLEIRKRLANAAENVRQGNSIHLSLEQTAIFPPMMLYMVAS
                     GEKSGQLGTLMVRAADNQETLQQNRIALTLSIFEPALIITMALIVLFIVVSVLQPLLQ
                     LNSMIN"
     gene            5465..5902
                     /gene="gspG"
                     /locus_tag="ACHKBL_003504"
     CDS             5465..5902
                     /gene="gspG"
                     /locus_tag="ACHKBL_003504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HFO5820324.1"
                     /translation="MRATDKQRGFTLLEIMVVIVIIGVLASLVVPNLMGNKEKADKQK
                     AVSDIVALENALDMYKLDNHRYPTTNQGLESLVEAPTLPPLAANYNKEGYIKRLPADP
                     WGNDYVLVNPGEHGAYDLLSAGPDGEMGTEDDITNWGLSKKKK"
     gene            5910..6419
                     /gene="gspH"
                     /locus_tag="ACHKBL_003505"
     CDS             5910..6419
                     /gene="gspH"
                     /locus_tag="ACHKBL_003505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspH"
                     /protein_id="HFO5820325.1"
                     /translation="MNQQRGFTLLEMMLVLALVAITASVVLFTYGREDAANTRARETA
                     ARFTAALELAIDRATLSGQPVGIHFSDSAWRIMVPGKTPSAWRWVPLQEDAADESKND
                     WDEELSIHLQPFKPDDSNQPQVVILADGQITPFSLLMANAGTGEPLLTLVCSGSWPLD
                     QTLARDTRP"
     gene            6416..6793
                     /gene="gspI"
                     /locus_tag="ACHKBL_003506"
     CDS             6416..6793
                     /gene="gspI"
                     /locus_tag="ACHKBL_003506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspI"
                     /protein_id="HFO5820326.1"
                     /translation="MNKQSGMTLLEVLLAMSIFATVALALMSSMQGQRNAIERMRNET
                     LALWIADNQLKSQDTFNDENTSSSGKEIINGEEWNWRSDVHSSKDGTLLERTITVTLP
                     NGQKTALTRYQSIHDRSGQAQDD"
     gene            6786..7373
                     /gene="gspJ"
                     /locus_tag="ACHKBL_003507"
     CDS             6786..7373
                     /gene="gspJ"
                     /locus_tag="ACHKBL_003507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspJ"
                     /protein_id="HFO5820327.1"
                     /translation="MINRQQGFTLLEVMAALAIFSMLGVLAFMVFSQASTLHQRSQKE
                     IQQFNQLQRTITILDNDLLQLVARRNRSTDKIMVLGEEAIFTTQSRDPQAPLNEAQTL
                     QTVHWYLRNHTLYRAVRTSVDGRKNQPAQAMLEHVESFLLESNSGESQELPLSVTLHL
                     KTQQYGALQRRFALPEQLAREESPAQTQAGNNNHE"
     gene            7366..8349
                     /gene="gspK"
                     /locus_tag="ACHKBL_003508"
     CDS             7366..8349
                     /gene="gspK"
                     /locus_tag="ACHKBL_003508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspK"
                     /protein_id="HFO5820328.1"
                     /translation="MNNEQRGVALLIVLMLLALMAALAADMTISFHGQLHRTRQVNHH
                     LQRQYDIELAEKLALASLTLDVKDNDRQTTLQQFWPQPQQLQLENGNTVKWQLRDAQH
                     CFNLNALAKISDAPLASPDFPAQVFSALLINAGIDRGNTDEIVQSIADYIDVDDSPRF
                     HGAEDNFYQSQTPPRHSANQMLFLTGELRQIKGITENIYQRLIPYVCVLPTTELSINL
                     NMLTENDIPLFRALFLNNITDADARVLLQKRPREGWLTTDAFLYWAQQDFSGVKPLVA
                     QVKGHLFPYSRYFTLSTESISDEQSQGWQSHIFFNRKQQSAQIYRRTLQLY"
     gene            8364..9527
                     /gene="gspL"
                     /locus_tag="ACHKBL_003509"
     CDS             8364..9527
                     /gene="gspL"
                     /locus_tag="ACHKBL_003509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspL"
                     /protein_id="HFO5820329.1"
                     /translation="MPESLMVIRSSSTLRKHWEWMTFSADSVSSVHTPTDDLPLESLA
                     DQPGAGNVHLLIPPEGLLYRSLTLPNAKYKLTAQTLQWLAEETLPDNTQDWHWTVVDK
                     QNESVEVIGIQSEKLSRYLERLHTAGLNVTRVLPDGCYLPWEVDSWTLVNQQTSWLIR
                     SAAHAFNELDEHWLQHLAAQFPPENMRCYGVAPHGVAATNPLIQHPEIPSLSLYSADI
                     AFQRYDMLHGVFRKQKTVSKSGKWLARLAVSCLVLAILSFVGSRGIALWQTLKIEDQL
                     QHQQQETWQRYFPQIKRTHNFRFYFKQQLAQQYPEAVPLLYHLQTLLLEHPELQLMEA
                     NYSQKQKSLTLKMSAKSEANIDRFCELTQSWLPMQKTEKDPVSGVWTVRSSGK"
     gene            9524..9985
                     /gene="gspM"
                     /locus_tag="ACHKBL_003510"
     CDS             9524..9985
                     /gene="gspM"
                     /locus_tag="ACHKBL_003510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001560018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspM"
                     /protein_id="HFO5820330.1"
                     /translation="MIKSWWAEKSTSEKQLVAALAVLSLGVFCWLGVIKPIDTYIAEH
                     QSHAQKIKKDIKWMQDQASTHGLLGHPALTQPIKNILLEEAKRENLAITLENGPDNTL
                     TIHPVTAPLENVSRWLTTAQITYGIVIEDLQFTLAGNEQITLKHLSFREQP"
     gene            9985..10662
                     /gene="gspO"
                     /locus_tag="ACHKBL_003511"
     CDS             9985..10662
                     /gene="gspO"
                     /locus_tag="ACHKBL_003511"
                     /EC_number="3.4.23.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase GspO"
                     /protein_id="HFO5820331.1"
                     /translation="MTMLLPLFILVGFIAGYFVNAMAYHLSPLEDKTALTFRQVLVHF
                     RQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGALFLYFCFALTLSVIDFRTQLLPD
                     KLTLPLLWLGLVFNAQSGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
                     LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLG
                     YLAWISY"
     gene            complement(10691..11167)
                     /gene="bfr"
                     /locus_tag="ACHKBL_003512"
     CDS             complement(10691..11167)
                     /gene="bfr"
                     /locus_tag="ACHKBL_003512"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000675505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HFO5820332.1"
                     /translation="MKGDTKVINYLNKLLGNELVAINQYFLHARMFKNWGLKRLNDVE
                     YHESIDEMKHADRYIERILFLEGLPNLQDLGKLNIGEDVEEMLRSDLALELDGAKNLR
                     EAIGYADSVHDYVSRDMMIEILRDEEGHIDWLETELDLIQKMGLQNYLQAQIREES"
     gene            complement(11239..>11428)
                     /gene="bfd"
                     /locus_tag="ACHKBL_003513"
     CDS             complement(11239..>11428)
                     /gene="bfd"
                     /locus_tag="ACHKBL_003513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HFO5820333.1"
                     /translation="VCLCNGISDKKIRQAVRQFSPHSFQQLKKFIPVGNQCGKCVRAA
                     REVMEDELMQLPEFKESA"
BASE COUNT         2979 a         2937 c         2734 g         2778 t
ORIGIN      
        1 ttttattaga acatacatcg tgcccacact acgcttcccc ttccatctgg cgaatcataa
       61 taaagatgct gccatcaata tattgattat atttataagc atagggttga ttatttttaa
      121 tgtgaattat ttccatacaa ctatagttaa aaatggacag atcattaatc aaccaacaaa
      181 cgcatttcag tctgagttca gccttgccgc tttatggcgt aacgaaaatc atgctggggt
      241 aaaagatgca aatcccgtgg cagttaatca ggaaacacct aagctctcta tagcgcttaa
      301 tggcattgta ctcacatcga atgatgagac ttcttttgtc ttaattaatg aggggagcga
      361 acaaaagcga tactccctca acgaagccct tgagtcagcg ccgggaacgt ttattagaaa
      421 aataaataaa accagcgttg tgtttgaaac tcacgggcat tacgaaaaag taactctcca
      481 tccagggcta cctgacatca tcaagcagcc tgattcagaa agtcaaaatg tactcgctga
      541 ttatattatc gccacgccga tacgtgatgg agaacagata tacgggcttc gattgaaccc
      601 tcgtaagggc ctcaatgcat ttaccaccag cttgctacaa ccaggggata tcgccctcag
      661 gatcaataat ctttcgctga cccaccctga tgaggtttca caggcattaa gtttactgct
      721 aactcaacaa agtgctcagt ttacaattcg tcgcaacggc gtaccccgct tgataaatgt
      781 ttccgtcgcg gaacttacag gaatgaatgg acagcgtcat gaaaggactc aataaaatca
      841 cctgctgctt gctggcagca ctactcatgc cttgtgcagg gcacgctgag aacgaacaat
      901 acggcgctaa cttcaataac gccgatatcc gccagttcgt ggaaatagtg ggtcagcatc
      961 ttggcaaaac gatcctgatc gacccttcgg tacagggaac catttccgta cgcagtaatg
     1021 atacgtttag ccaacaggag tactaccagt tctttttaag tattcttgat ctttacggtt
     1081 attccgtgat cacgctggac aatggttttc tgaaagtggt gcgctcagct aatgtaaaaa
     1141 catcgccagg gatgattgct gacagttctc gtccaggcgt aggtgatgag ttggtcaccc
     1201 gaatcgtacc gcttgagaac gttcctgctc gtgacctggc tcccctgctc cgccagatga
     1261 tggatgcggg tagcgtcggt aatgttgtgc attatgaacc ctccaacgtt cttattctga
     1321 ccggtcgtgc ctccaccatt aataaactga ttgaagtcat aaagcgcgtt gatgtcatcg
     1381 gcacagagaa gcagcaaatt attcatctgg aatatgcgtc agcggaagat ctcgccgaaa
     1441 ttttgaatca attaatcagc gaaagccacg gtaaaagcca gatgcctgcc ctcctttccg
     1501 cgaagattgt ggcggataag cgaaccaact ctcttatcat cagcgggccg gaaaaagcac
     1561 gccagcgcat cacttcatta ctgaaaagcc ttgatgtaga agagagcgag gaaggaaata
     1621 ctcgggttta ttacctgaaa tatgctaaag ccacgaatct ggtggaagtg ctaaccggtg
     1681 tttccgaaaa gctgaaagat gaaaaaggga atgagcgtaa gccctcctct tctggcgcga
     1741 tggataacgt cgccattacc gccgatgaac agactaactc tctggtcatt accgctgacc
     1801 agtccgtcca ggaaaaactc gccacggtaa ttgcgcgtct ggacattcgc cgtgcacagg
     1861 tgctggttga ggcaatcatc gttgaagttc aggatggaaa tggacttaac ctcggcgtgc
     1921 aatgggcgaa taaaaacgtt ggagcacagc aatttaccaa taccggatta ccgattttta
     1981 acgctgcgca aggtgtggct gattataaaa agaatggtgg gatcaccagc gcgaatcctg
     2041 cctgggatat gtttagcgcc tacaatggca tggccgcagg ctttttcaat ggcgactggg
     2101 gcgtattgtt gactgcgctg gccagtaaca ataaaaatga catcctcgcc accccgagca
     2161 tcgtaacgct ggataataaa ctcgcgtcct tcaacgttgg tcaggatgtg ccggtgctat
     2221 ctggctctca gaccacttct ggggataacg tctttaatac tgtcgaacgc aaaacggtgg
     2281 gcacaaaact caaagtcact ccgcaggtca atgaaggcga cgcggtactg ctcgaaatag
     2341 agcaggaggt ttccagcgtt gactcttcgt ctaattcgac gctcggcccg acatttaaca
     2401 cccgaactat tcaaaatgcc gtgctggtca aaaccggtga aacggtggtc ctgggcggat
     2461 tgctggatga tttttctaaa gagcaagtgt caaaggttcc tctgcttggc gatattcctt
     2521 tagtggggca actcttccgc tatacttcga ccgagcgcgc taaacgcaac ctgatggtat
     2581 ttatccgccc aacgattatc cgtgacgatg atgtttatcg ctcactgtca aaagagaaat
     2641 acacccgcta ccgtcaggag caacaacagc gaatcgacgg gaaatcaaaa gcgctggttg
     2701 gctcggaaga tttgccggtg ctggatgaga acacgttcaa cagccatacc cctgcgccct
     2761 cggcacggta aggcattctt atgagaattc actcaccgta ccccgccagt tgggcgctgg
     2821 cacaacgaat tggttatctc tattcagagg gcgagattat ttatctcgcc gatacgccat
     2881 tcgaacggtt actcgatatt caacgtcagg ttggccagtg ccagaccatg accagcttgt
     2941 cacaggctga ttttgaagcc cggctggaag cggtgttcca tcagaatacc ggtgagtcgc
     3001 aacagattgc gcaggatatc gatcaatccg tcgatcttct ctcgctttcg gaagagatgc
     3061 ccgcaaatga agatctcctg aatgaagatt cagcggcacc ggttatccgc ttgatcaatg
     3121 cgattttgag tgaggccatc aaagaaaccg cctccgatat ccacattgaa acctatgaaa
     3181 aaacaatgtc gatccgtttt cgcatcgacg gtgttttgcg gacaatttta cagccaaaca
     3241 aaaaactggc ggcactgctt atctcccgaa ttaaggtcat ggctcgtctt gatatcgccg
     3301 aaaaacgtat tccacaggat ggacgtatta gtttgcgtat cgggcgacgt aacatagatg
     3361 tccgcgtatc cacactgccg tccatctatg gtgaacgcgc cgtgctccgt ctgctggata
     3421 aaaacagcct ccagctttca ttgaacaacc tggggatgac ggcagcggat aaacaggatt
     3481 tagaaaatct catccagctt ccacacggta ttatcctggt gaccggaccg acaggctccg
     3541 gtaaaagcac cacgctctac gccatccttt cggcgctgaa tactcccggc cgcaatattc
     3601 tgacggtaga agatcccgtg gaatatgagc tggaaggcat tgggcaaacg caggtgaata
     3661 cccgtgtgga tatgtctttc gctcgcggcc tgcgcgccat acttcgccag gacccggatg
     3721 tcgtcatggt gggggaaatt cgtgatacag aaaccgcgca gattgcggtt caggcctcgc
     3781 tcaccggcca tctggtactc tcaacactcc acactaacag tgcatcaggc gcagtgaccc
     3841 ggctccgcga catgggcgtc gaatcattcc tgctttcgtc ttccctggca gggattatcg
     3901 cgcaacgtct ggttcgtcgc ctgtgtccgc aatgccgaca attcacgccc gtatcacccc
     3961 aacaagcgca gatgtttaaa tatcatcagc tcgcggtgac aacaattggc actcccgtag
     4021 gctgccctca ttgccatcaa tccggctatc aggggcgcat ggcgatccac gaaatgatgg
     4081 tggtgacgcc ggaattacgg gccgctattc atgaaaatgt ggatgaacaa gcactggagc
     4141 gactggtccg gcaacaacac aatgccttaa tcaaaaatgg cctgcaaaaa gtgataagcg
     4201 gtgacacctc ctgggatgag gttatgcgcg tcgccagtgc cacgctggag aacgaagcat
     4261 gaattatcgc tatcgcgcca tgacccagga tggtcaaaaa ttgcagggga tcattgatgc
     4321 taacgatgaa cgtcaggcac gactgcggct gcgtgaagaa gggcttttcc tgctggatat
     4381 tcgcccccaa aaaagttcgg gagtaaaaac acgtcgcccg aggatcagcc atagtgaact
     4441 gacgcttttc acccggcagt tggcaacctt aagcgcagcg gcattacctc tggaagagag
     4501 ccttgccgta atcggtcaac aaagcagtaa taaacgactg gctgatgtgt taaatcaggt
     4561 acgcagcgcc atccttgaag ggcatcccct ttccgatgcg ttacaacatt ttcccacgct
     4621 tttcgattcg ctctaccgta ccctggttaa agcgggcgaa aagagcgggc tgctggcccc
     4681 ggtgttggaa aaactggctg attacaatga aaatcggcag aaaatccgca gcaaactcat
     4741 tcagtcgctg atctacccct gcatgctgac tgcggtggcg attgtggtcg tgattattct
     4801 cctcactgct gtcgtgccca aaattaccga acagttcgtg catatgaagc agcaactgcc
     4861 gctgagtaca cgcattcttt taggtctgag cgacacgttg caacataccg gcccgacatt
     4921 actagcgaca gtgtttattg tcgctgtagg tttctggctc tggttaaaac gcggcaataa
     4981 ccgccaccgt tttcatgcca tgttgctgcg cgttgcactc atcggcccgc tgatttgcgc
     5041 cattaacagc gcacgctatc tccgcacttt aagtattttg caatccagcg gcgtccctct
     5101 gctggatggg atgaatttgt ccaccgaaag tctcagcaac ctcgaaatcc gcaagcgtct
     5161 ggcaaatgcg gcagaaaacg ttcggcaggg taacagcatt catctttcgc tggaacaaac
     5221 ggcaatcttc ccgccgatga tgctctacat ggtggcctct ggcgaaaaaa gcgggcagct
     5281 cggcacgtta atggtcagag cagcagataa ccaggagaca ctccaacaaa atcggatcgc
     5341 cttaacgctc tccatcttcg agccagcact cattattacg atggcactga tcgtcctgtt
     5401 tattgtcgtc tcggtactcc aacctcttct tcaacttaac tcaatgatta attaaggaaa
     5461 gtatatgcgc gcaacggata agcaacgcgg ttttacatta ctggaaatta tggtggtgat
     5521 cgtcatcatt ggcgtacttg ccagcctggt ggttcccaac ctgatgggca ataaagaaaa
     5581 ggcggataaa caaaaagccg tcagcgatat tgtggcgctg gaaaacgccc tggatatgta
     5641 caagctcgac aatcatcgct atccaaccac aaatcaggga cttgaatcct tagtcgaagc
     5701 gccgacactg ccaccgctgg ccgcaaacta taacaaggaa ggttatatca agcgtctgcc
     5761 tgccgatccc tggggcaatg attatgtcct cgttaatcct ggtgaacatg gtgcatacga
     5821 tctgctttca gcagggcccg atggtgaaat gggaaccgag gacgacatca ccaactgggg
     5881 tttgagcaag aagaaaaagt aagtcagcga tgaatcagca acgcgggttt accctgctgg
     5941 agatgatgct ggtgctggcg ctggtcgcta tcacggcaag tgtggtgctc ttcacatatg
     6001 gtcgggagga tgccgccaac actcgtgcgc gcgaaacagc ggcgcggttt accgctgcac
     6061 ttgaattagc catcgaccgc gctacgctca gtggtcagcc tgtcggtatc catttctctg
     6121 attcagcatg gcgcattatg gtaccgggca aaacaccatc agcctggcgc tgggttccat
     6181 tacaagaaga tgccgcagac gagagcaaaa atgactggga tgaagaactc tcaattcatc
     6241 ttcagccatt taaacctgat gacagcaacc aaccgcaggt ggtgattctg gccgacggac
     6301 aaatcacgcc cttctctttg ttgatggcta acgcagggac cggtgagccg ctcctgacgt
     6361 tagtctgttc aggatcatgg cccctggatc aaacgctcgc cagagatacc cgcccatgaa
     6421 caaacaatca gggatgacac tgcttgaagt cttactggcg atgagtattt tcgccactgt
     6481 tgcgctggct ttaatgagca gcatgcaagg gcaacgaaat gccatcgagc ggatgcgtaa
     6541 tgagacgctg gcgctgtgga tcgcagataa ccagcttaag tcgcaagata cattcaacga
     6601 tgaaaacacg tccagttcag gcaaagagat aattaatggc gaagagtgga actggcgcag
     6661 cgatgtccat tcaagcaaag acggcacgct tctggaacgc accataacgg tgacgttacc
     6721 caacggtcag aaaaccgctc tgactcgtta tcagagcatc catgatagat ccgggcaggc
     6781 gcaagatgat taatcgccaa cagggtttca cattgctgga agtcatggcg gcactggcga
     6841 tcttctcaat gcttggcgtg ctggcgttta tggttttttc gcaagcgtct acgctacatc
     6901 aacgctcaca gaaagaaatc cagcagttca atcaactgca acgcaccatc accattcttg
     6961 ataacgatct cctgcaactg gtcgctcgcc gaaatcggag tacggataaa atcatggtgt
     7021 tgggtgaaga ggctattttt accacgcaaa gtcgcgatcc gcaggccccg ctcaatgaag
     7081 cgcaaacctt acaaacggtt cactggtatc tgcgaaatca tacgctttac cgtgctgttc
     7141 gtacttctgt ggatggcagg aagaatcaac ctgcccaggc gatgctggag catgtcgaga
     7201 gctttcttct ggaaagtaac agcggggaaa gccaggagct tcctctctca gtcacgttac
     7261 atctgaagac tcagcaatat ggggcgttac agcgccgttt tgcattacct gagcagttag
     7321 cgagagaaga gtcgccagct cagacgcagg ccgggaataa taaccatgaa taatgaacaa
     7381 cgcggcgtag cactgctcat tgtactcatg ttactggcgc tcatggctgc tctggctgca
     7441 gacatgacga tcagttttca cggccagctt catcgaaccc gccaggtaaa ccatcatttg
     7501 caacggcaat atgatattga gctggcggag aaacttgcgc ttgccagcct caccctggac
     7561 gttaaagata acgacagaca aaccacgttg cagcagttct ggccacaacc gcaacagttg
     7621 cagctggaaa atggcaatac agtgaagtgg cagttacgcg atgcccagca ctgttttaat
     7681 ctcaatgcct tagcaaaaat ctctgacgcc ccgctggctt cccctgattt ccccgcccag
     7741 gtattcagtg ccctgctgat taacgccggt atcgatcgcg gaaacaccga tgagatcgtg
     7801 caatcaattg ctgactatat tgatgtcgat gactcaccac gctttcacgg cgcagaagat
     7861 aacttttacc aaagccagac gccgccccgg catagtgcca atcagatgct ttttctgacc
     7921 ggcgaattgc gtcagataaa aggcataacc gaaaacatct atcagcggct tatcccgtat
     7981 gtctgcgttc tcccaaccac ggaacttagc atcaatctta atatgcttac ggagaacgat
     8041 attccgctct tcagagcgtt attcctcaat aacatcacgg atgctgacgc tcgggtctta
     8101 ttacaaaaaa ggcccaggga aggctggcta accacggatg catttcttta ctgggcgcag
     8161 caggatttct ccggcgtgaa gcctctggta gctcaggtga aagggcatct ctttccctat
     8221 agccgctatt ttacgctgag tacggaaagt atcagcgacg aacaatccca gggatggcaa
     8281 agccatattt tcttcaaccg taaacagcag agcgcccaaa tctatcggcg tacgctgcaa
     8341 ctctactaat cataaggatc gtgatgcctg aatcgttgat ggtcatacgt tcttcctcga
     8401 cgctgcgtaa gcattgggaa tggatgacgt tctccgcaga tagcgtatct tcggtacata
     8461 cgcccacgga cgatcttccg ctggagtcgc tggccgatca gcccggtgcc ggaaatgttc
     8521 atcttttgat cccaccagag ggccttttat atcgttccct cacgctgccc aacgccaaat
     8581 acaaactcac cgcacaaaca ttgcaatggc tggcagagga gacgttaccc gacaacaccc
     8641 aggactggca ctggacggtt gtcgataaac aaaacgaaag tgtcgaggtg ataggcattc
     8701 agtcggagaa actcagtcgc tatcttgagc gtttacatac tgccggactc aacgtgacgc
     8761 gggttttgcc agatggctgt tatcttccct gggaagtaga tagctggact ctggttaacc
     8821 agcaaacaag ctggcttatt cgcagtgccg cacatgcctt caatgaactg gatgaacact
     8881 ggttacagca tcttgctgcc cagtttccgc ctgagaatat gcgttgttat ggtgtggcac
     8941 ctcacggcgt tgcagcgacc aatccactta tccagcatcc tgaaatacca tcactgagtt
     9001 tgtattctgc cgatattgca ttccagcgct atgacatgct gcatggtgtt tttcgtaaac
     9061 agaaaactgt aagcaaatcg ggaaaatggc tcgcccgcct ggcagtaagc tgtctcgttc
     9121 tcgccattct ttcttttgtt ggcagccggg gcatcgcgct ctggcaaacg cttaagattg
     9181 aagatcaact tcagcatcaa cagcaagaaa cctggcaacg ttatttcccg cagatcaaac
     9241 gcacccacaa ctttcgtttt tactttaagc agcaactcgc tcagcaatat cctgaagctg
     9301 tgccgttgct ctatcattta caaacacttc tgctggaaca ccctgaactg cagttaatgg
     9361 aggcgaacta tagccagaag caaaagtcgc tgaccttaaa aatgagtgct aagagtgaag
     9421 ccaatatcga tcgtttctgt gaattaacac agtcctggct tccgatgcaa aaaacagaaa
     9481 aagatccggt cagtggtgta tggacagtaa ggagctcagg gaaatgatta aatcatggtg
     9541 ggcagaaaaa tccacatcgg aaaaacagct cgttgcggcg ctggcggttc tctcgctcgg
     9601 cgttttttgt tggcttgggg tgataaaacc gatagatacc tacattgcag agcatcagtc
     9661 ccacgcgcaa aaaattaaga aggacatcaa atggatgcag gatcaagcga gcacccatgg
     9721 tttactcggt catcctgcgc ttacgcagcc catcaaaaat atccttctcg aggaagcaaa
     9781 acgtgagaat ttggctatca cgctggaaaa cggtccggac aatactctga caattcatcc
     9841 tgtcacagct cccctcgaaa acgtctcccg ctggctcact acggcacaga ttacatacgg
     9901 tattgttatt gaagaccttc agtttactct tgccggaaac gaacagatca ccctcaagca
     9961 tttgtccttc agggagcaac cataatgaca atgctactgc cgctcttcat tctggttggt
    10021 tttattgcag gttattttgt taatgccatg gcctatcacc tctcgccgtt ggaggataaa
    10081 acggcgttaa cgtttcgcca ggtattggtt catttcaggc aaaaaaaata tgcctggcat
    10141 gatacagtgc ccctgatact ttgcgttgct gctgctatcg cctgcgccct ggcacctttc
    10201 acgcccatcg tgactggcgc actctttctc tatttctgtt tcgcactcac gctcagtgtt
    10261 attgattttc gcactcagct cctgcccgac aaactcacct taccgctgct ctggcttggc
    10321 ttggtattta atgcgcagtc tggattaatt gatttacatg atgcggttta cggcgcggta
    10381 gctggctatg gggtgctgtg gtgtgtttac tggggcgtct ggttagtttg tcacaaagag
    10441 ggattgggct acggtgattt caagctactg gccgctgcag gcgcgtggtg tggctggcaa
    10501 acgttgccaa tgatactgct aattgcctca ctgggtggca ttggttacgc catcgtttca
    10561 caacttctgc aacgccggac tataaccact atcgcattcg gcccgtggct tgcgctcggc
    10621 agcatgataa acctggggta tctggcctgg atctcgtatt aaccgggagg gttctccctc
    10681 ccgacacggc tcaactttct tcgcggatct gtgcttgcag ataattttgc aggcccatct
    10741 tctgaatcag atcaagttcc gtttccagcc agtcgatatg gccttcttca tcacgcaaaa
    10801 tttctatcat catatcgcgg ctgacgtaat catgaacgct atcggcataa ccaattgcct
    10861 cacgcaaatt cttcgcgcca tccagctcaa gtgccagatc agaacgcagc atttcctcaa
    10921 catcctcacc aatgttcagt ttgcccaggt cctgtaaatt tggaagacct tccagaaaaa
    10981 gaatgcgctc aatataacga tcggcgtgtt tcatctcatc aatggattca tgatactcca
    11041 catcattgag acgtttgaga ccccagtttt taaacatccg ggcatggaga aagtactgat
    11101 tgattgcgac aagctcattt cccaacagtt tgttgagata atttataacc ttagtatcac
    11161 ctttcatttt ttgactcctt cgcttccact ctttgaagcg tagaacggct tacgagtaag
    11221 tcaaaaaaca cactccgttt atgcggactc cttaaactcc ggcaactgca ttaattcatc
    11281 ctccatcact tcacgcgcgg cccggacaca tttaccgcac tgattgccga ccggaataaa
    11341 tttttttaat tgctggaacg agtgagggga aaactggcgc acagcctgac gaattttttt
    11401 atcgctgata ccattacaaa gacaaacg
//
LOCUS       DAZFLD010000151        11417 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.152, whole genome shotgun sequence.
ACCESSION   DAZFLD010000151 DAZFLD010000000
VERSION     DAZFLD010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11417
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.152"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(135..1070)
                     /locus_tag="ACHKBL_003514"
                     /pseudo
     CDS             complement(135..1070)
                     /locus_tag="ACHKBL_003514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011787828.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            complement(1414..2034)
                     /gene="entD"
                     /locus_tag="ACHKBL_003515"
     CDS             complement(1414..2034)
                     /gene="entD"
                     /locus_tag="ACHKBL_003515"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009366 - enterobactin synthetase complex
                     [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009239 - enterobactin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin synthase subunit EntD"
                     /protein_id="HFO5820334.1"
                     /translation="MKTTHTSLPFAGHTLHFVEFEPASFHEQDLLWLPHYAQLQHAGR
                     KRKAEHLAGRIAAVHALREFGFKYAPPVGERRQPLWPAGLYGSISHCQATALAVVSHQ
                     PTGIDIEEIFSAQAAEELKDSIMTPAEYELLVVSNLPFSVVLTLAFSAKESAFKASKL
                     QTDQGFLDYQIVNWNKQRIIICWENEIFAVQWQIRGKQLITLCQHD"
     gene            complement(2188..3536)
                     /locus_tag="ACHKBL_003516"
     CDS             complement(join(2188..3056,3056..3536))
                     /locus_tag="ACHKBL_003516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558234.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFO5820335.1"
                     /translation="MKHSFEVKLAAVNHYLAGHAGIISTAKLFQLSHTSLSHWINLFL
                     LHGPRALDCRHKRSYSPEDKLCVVLYALGHSESLPRVAARFNIPSHNMVKNWIKGYRK
                     SGNEAFIRRRKEKSMTRSDDTHENEANMTPEEMKNELRYLRAENAYLKAMQEHLLEKK
                     PPGAGEKTKVIRSLKCGHCQSDLLKAAGLARSTLYYQLSLQKAKDKYADVKQLIASIF
                     HEHRGCYGYRRIHCELQKRGLKFSGKTVRKLMQQLGLKSPVRLKKYRSYRGNMGLAAE
                     NILQRQFKAEAPCEKWVTDITEFRAGGQKLYLSPILDLFNGEIVAWETACRPTEELVK
                     RMLNKGLESLAEGEKPLLHNDQGWHYRIKSYQSALADKGLVQSMSRKGNCLDNAVMEN
                     FLGHLKEEMYYRRDYRSVEELENAVNEYITYWNQKRIKLSLGGLSPLEYRTEYQKAG"
     gene            complement(3646..5886)
                     /gene="fepA"
                     /locus_tag="ACHKBL_003517"
                     /pseudo
     CDS             complement(3646..5886)
                     /gene="fepA"
                     /locus_tag="ACHKBL_003517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308650.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore enterobactin receptor FepA"
     gene            6129..7331
                     /gene="fes"
                     /locus_tag="ACHKBL_003518"
     CDS             6129..7331
                     /gene="fes"
                     /locus_tag="ACHKBL_003518"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0008849 - enterochelin esterase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterochelin esterase"
                     /protein_id="HFO5820336.1"
                     /translation="MTALKVGSESWWQSKHGPEWQRLNDEMFEVTFRWRDPQGSEEYS
                     TIKRVWVYITGVTDHHQNSQPQSMQRIAGTNVWQWTTQLNANWRGSYCFIPTERDDIF
                     SAPSPDRLELREGWRKLLPQAIADPLNPQSWKGGRGHAVSALEMPQAPLQPGWDCPQA
                     PETPAKEIIWKSERLKNSRRVWIFTTGDATAGERPLAVLLDGEFWAQSMPVWPVLTSL
                     THRQQLPPAVYVLIDAIDTTHRAHELPCNADFWLAVQQELLPLVKAIAPFSDRADRTV
                     VAGQSFGGLSALYAGLHWPERFGCVLSQSGSYWWPHRGGQQEGVLLEQLKAGQVSAEG
                     LRIVLEAGIREPMIMQANQALYAQLHPIKESIFWRQVDGGHDALCWRGGLMQGLIDLW
                     QPLFHDRS"
     gene            7334..7552
                     /gene="ybdZ"
                     /locus_tag="ACHKBL_003519"
     CDS             7334..7552
                     /gene="ybdZ"
                     /locus_tag="ACHKBL_003519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis protein YbdZ"
                     /protein_id="HFO5820337.1"
                     /translation="MAFSNPFDDPQGAFYILRNAQGQFSLWPQQCALPAGWDVVCQPQ
                     SQVSCQQWLEAHWRTLTPANFTQLQEAQ"
     gene            7549..>11417
                     /gene="entF"
                     /locus_tag="ACHKBL_003520"
     CDS             7549..>11417
                     /gene="entF"
                     /locus_tag="ACHKBL_003520"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0031177 - phosphopantetheine binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin non-ribosomal peptide synthetase
                     EntF"
                     /protein_id="HFO5820338.1"
                     /translation="MSQHLPLVAAQPGIWMAEKLSDLPSAWSVAHYVELTGEVDAPLL
                     ARAVVAGLAQADTLRMRFTEDNGEVWQWVDDALTFELPETIDLRTNIDPHGTAQSLMQ
                     ADLQQDLRVDSGKPLVFHQLIQVADNRWYWYQRYHHLLVDGFSFPAITRQIANIYCAW
                     LRGEPTPASPFTPFADVVEEYQQYRESEAWQRDAAFWAEQRRQLPPPASLSPAPLPGR
                     SASADILRLKLEFTDGEFRQLATQLSGVQRTDLALALTALWLGRLCNRMDYAAGFIFM
                     RRLGSAALTATGPVLNVLPLGIHIAAQETLPQLATRLAAQLKKMRRHQRYDAEQIVRD
                     SGRAAGEEPLFGPVLNIKVFDYQLDIPGVQAQTHTLATGPVNDLELALFPDEHGDLSI
                     EILANKQRYAEPTLIQHAERLKMLIAQFAADPALLCGDVDIMLPGEYAQLAQINATQV
                     EIPETTLSALVAEQAAKTPHAPALADARYLFSYREMREQVVALANLLRERGVKPGDSV
                     AVALPRSVFLTLALHAIVEAGAAWLPLDTGYPDDRLKMMLEDARPSLLITTDDQLPRF
                     ADVPDLTSLCYNAPLTPQGSAPLKLSQPHHTAYIIFTSGSTGRPKGVMVGQTAIVNRL
                     LWMQNHYPLTGEDVVAQKTPCSFDVSVWEFFWPFIAGAKLVMAEPEAHRDPLAMQQFF
                     AEYGVTTTHFVPSMLAAFVASLTPQTARQNCATLKQVFCSGEALPADLCREWQQLTSV
                     PLHNLYGPTEAAVDVSWYPAFGEELAQVRGSSVPIGYPVWNTGLRILDAMMHPVPPGV
                     AGDLYLTGIQLAQGYLGRPDLTASRFIADPFAPGERMYRTGDVARWLDNGAVEYLGRS
                     DDQLKIRGQRIELGEIDRVMQALPDVEQAVTHACVINQAAATGGDARQLVGYLVSQSG
                     LPLDTSALQAQLRETLPPHMVPVVLLQLPQLPLSANGKLDRKALPLPELKAQAPGRAP
                     KAGSETIIAAAFSSLLGCDVQDADADFFALGGHSLLAMKLAAQLSRQFVRQVTPGQVM
                     VASTVAKLATIIDGEEDSTQRMGFETILPLREGNGPTLFCFHPASGFAWQFSVLSRYL
                     DSQWSIIGIQSPRPHGPMQTAANLDEVCEAHLATLLEQQPHGPYYLLGYSLGGTLAQG
                     IAARLRARGEQVAFLGLLDTWPPETQNWQEKEANGLDPEVLAEINREREAFLAAQQGS
                     TSTELFTTIEGNYADAVRLLTTAHSVPFDGKATLFVAERALQEGMNPERAWSPWIAEL
                     DIYRQDCAHVDIISPGAFEKIGPIIRAT"
BASE COUNT         2507 a         3027 c         3008 g         2875 t
ORIGIN      
        1 tgttgttgaa atttttatga gctatttacg cgcccacata ttattcaagt cttgcatata
       61 tatgctgagt agttaaattc ccctgtgcta tgccagttac tccattcgct tctaagtagc
      121 tgatcctaat accgctacac tttagccagc ccatgctttc caccggggaa tgggctgacg
      181 gtatccataa agtgacgaac ctttttcaac agttgccaca ttgaacggca ctggtgattt
      241 cgggttattg tttcatgaag cgcctgccac aaccgttcga catgattgac ccatggcgag
      301 taaattggct gataaatcac cctgaacttt gggtttgctt tcagccagcg caacgtttca
      361 cagcttttgt gggtgatgta gttatcgaca atgagcgtaa tcttttttgc ccgtcagtat
      421 gtctccttca gatgttttaa cagcctgata aataaggatg aacctttact gtttccaccc
      481 acgtagctga ctttgcccgt accactgtgc agcgcaccgg caagatagta cttttcattc
      541 cggcccggcg taacaacgcg cttttgctgc ccgcgtaact gccagtctgc gccgattttg
      601 ggattgaggt ggatctccac ttcatcttcg taaaacacag ggtgttctat gctacaactg
      661 actaacgcta cattaatcgc tgccattttt tcttccttat tcgggccccg gatacgcagt
      721 gttggtgctg ctctgcgcca taccagttcg gctgcaggta accatcgccg ggagaatgct
      781 taacaagctc acgcagcaat gcgcaaatat gctcgaacgg ccagcgcctc tcacgtcctg
      841 acggcaatga cttcaggcct tcaacacccg acagcgtgaa ccagttaatc cagcgaccaa
      901 tcgatgagcg agcacagcag agtgttctgg cagcatgact gacggtgtca ccacgatgca
      961 gcatcagcat ggctgtgagc cgccgggcgt ggttttttgt cctttgtttt atggatcgtt
     1021 ttctggatca ggcgtcgttc agttctgggt attggtgcta tgatcggcac tgctccgtcc
     1081 ggttggtgat ttgggatatt cagcgattga tcagatcgca taatcgactc tgtaacaacc
     1141 tgaacggtgt cggggtggta acataggtta ggaaacaggg tgcggatgcc atcatggcgc
     1201 gggtcatcag tccactaacg acgccggata tatggcagca aatcagtggt tgtatattga
     1261 acaactataa tagcgtcaga tctcaccatt ataacggtgg gctgacaccg aaaaaattag
     1321 agaacagata acgttcttag cgtaaaacgg tggtcaatat tacttgacta ctacaagcaa
     1381 tggttgctgg ctaaccacag taagggttat ccattaatcg tgttgacaaa gcgttatgag
     1441 ttgttttcct cttatctgcc attgcacagc aaatatctcg ttctcccaac aaataatgat
     1501 ccgctgttta ttccagttca ctatctgata atccagaaaa ccttgatctg tctgtaactt
     1561 gcttgcctta aacgcactct ctttcgctga aaacgccagt gtaagcacca cagaaaaggg
     1621 taaattgctg accacaagca gctcgtattc tgctggtgtc ataatgctgt ctttcaactc
     1681 ttccgctgct tgtgcagaga agatctcttc gatatcaata cccgttggtt gatgggatac
     1741 caccgctaat gccgtagcct ggcagtgact aatactgccg tataacccgg caggccaaag
     1801 tggctggcga cgttcaccga cagggggggc gtacttaaag ccaaattccc gtaaagcatg
     1861 aacagcagca atccgtccgg ctaaatgttc ggctttgcgt ttccgtccgg catgttgcag
     1921 ttgcgcataa tgcggcagcc agagtaaatc ctgttcgtga aaacttgctg gctcgaactc
     1981 aacaaaatgc agcgtatgtc cggcaaaggg gagggaggta tgcgtagttt tcatatcgac
     2041 catattagag actgatgaca aacgcaaaac tgcctgatgc gctacgctta tcaggcctac
     2101 actatcctgc aatatattga attggcaaga tttttgtagg ccggataagg cgttctgaac
     2161 tgaaccccaa aacctggaca gtttcagtta accggctttt tgatactcag tccggtattc
     2221 taacgggctg agtcccccaa gactgagttt tattcttttt tgattccagt aagttatgta
     2281 ttcgttaacg gcattttcca gctcttctac acttctgtag tcccggcgat agtacatttc
     2341 ttctttcagg tgacctaaga aattctccat taccgcatta tcaagacagt tgcccttgcg
     2401 tgacatgctc tgcactaacc ctttgtctgc cagggcagac tgataacttt ttatccggta
     2461 atgccaccct tgatcattgt ggagcagcgg tttttctcct tctgcaaggc tttccagccc
     2521 cttatttaac atgcgcttca ccagctcctc tgtaggcctg caggcagttt cccacgccac
     2581 gatttccccg ttaaacaaat caagtatcgg ggacaggtac agtttttgtc cgccagccct
     2641 gaattcggtg atatctgtca cccatttctc acagggagct tccgctttga actgccgctg
     2701 aaggatattt tctgcagcga gtcccatatt tccccggtaa gaccggtatt tcttcaggcg
     2761 cacaggagac ttcaggccga gctgttgcat cagttttcgc accgttttgc cactgaactt
     2821 aagacctctt ttctgaagct cacaatggat ccgccggtac ccataacatc cccggtgttc
     2881 atggaagatg gaggctatga gctgtttcac atcagcatac ttgtccttag ccttctgcag
     2941 tgacagctgg tagtacagcg tgcttctggc cagccctgca gcctttaaca ggtcagactg
     3001 gcagtgtccg cacttcaggc tccggatgac tttcgttttt tctccagctc ctggcgcttt
     3061 ttttccagga gatgctcctg catggctttc agatatgcat tttctgcgcg aagataacgc
     3121 agctcattct tcatctcttc cggcgtcatg tttgcctcgt tttcatgggt atcatcagaa
     3181 cgggtcatgc ttttctcctt cctgcgcctg ataaaggcct cattaccaga tttgcggtag
     3241 ccttttatcc agtttttaac catgttgtgg ctgggaatgt taaaccgggc agctacccgg
     3301 ggtagagact cagaatgccc gagagcataa agaaccacgc aaagtttatc ttcaggagaa
     3361 tagctacgct tgtgtctgca atccagtgcc cgaggaccgt gaagaagaaa aagattaatc
     3421 cagtgtgaga ggctggtgtg agaaagttga aagagttttg ccgtagaaat gatgcctgca
     3481 tgaccagcca ggtaatgatt tacagcggca agttttactt caaatgaatg tttcataaac
     3541 tgcaccttca gatgtcagat tatgtccaac atttggggtg cagttcagtt tcacgccgca
     3601 tccggcatga attacgcgca ctttgtcaac aatctgacgt tagcatcaga agtgagtatt
     3661 aatgctcata taccacgtac gtcccggctc gttataggta tacgccccgg caccggcgat
     3721 atagttggct cctgccagat cgcccgtggt ctgggcatta cccgcacgcc acaaacgttt
     3781 gtcgaacagg ttgtccacgc cgccggtcag actgacattc ttcgtcacat cccaggtcgc
     3841 gctcagaccc acaatgctgt aaggactgat ttctttggtt tccggtccaa ccgctggctg
     3901 acctttatag ttgtacttct tcggctgctg cttgccgtac caggtgaagg ttgtttgcat
     3961 cgacaaatct tcccgtgcct gccagctcag cgttgagttc aacgtatact ccgggatgat
     4021 cgacaaacgg tcgcccgtgg ttttgttttc actcttcagc atataagtga tgttattggt
     4081 ccacatcacc gtttcgctaa ccggtacgtt taacgatcct tccagacctt caaccaccgc
     4141 tttcggtacg ttatcccact gatagagatc ggtgccgact gcgttttgcc ctacagccac
     4201 atagcccgct tcaatcttat tgcgataatc gttacggaac caggtgacgc ccgccagcca
     4261 accgtcgcgt ttgaactcca gaccaatctc tttgttgatg ctggtttccg ctttcaggtc
     4321 atcattacct tgcagatagc agccgcccgc gctggcatag cagccctgac ctttactgta
     4381 gagaatgtag tttgggttag tctggtacag actcggcgct ttataagcgc gagcaatgcc
     4441 cattttcagc gtgaagtcat cgcctaaacc ttgcgatatg ttcagcgccg gactccagtt
     4501 attgccgaca atactgtgat gatcgaaacg cagccccggc gttacgatgg tgctgtcagt
     4561 tagctccata ttgttttcgg caaacagcga gaaaatttct gcttttgaat acgggctacg
     4621 gtcggtggca ctcacgccat caatagcgcc gccggtattc gtcccggtga gcgcttgggt
     4681 gttggaactc aagtccttca tccgctgctg attccactcc gtacccagcg tcagcgtctg
     4741 gttaacgagg aaatcaatcg gcaggttaac ttcgctgtgc agcatcacgt catcgagatc
     4801 gatatcgacg aaatcctgtg tcgctttttc gttaaatttc ccttcggtac cgcccgccag
     4861 accttccgga atacgcgagt tacgggtgtg ttcgtactgc acccagttgc tggtggtcac
     4921 gccgttatcc cagccgccgt tccatgtcag cgagtagttc tggcgataca gacggttggt
     4981 ttcatcgcca tatttcgagc gggtatagga gtcagagttg gtgttctggg tatcgcccgc
     5041 atacaggtta ccctggcggc tgtaacccgc ttccagttcc agcgattgca atggtgcgaa
     5101 gtcccagcgc accacgccgt taatatcttt gttgatcacc ccttcgcgcc cggctggtaa
     5161 cgtcgtggca tacgttccgg cacgcgcgga ctgatggccc tggttgatat cccacgcgtc
     5221 agcctgagtt ttgtcgaggt tgccatacaa gcggaagctg aattcgtcgc ccagcggacc
     5281 ggtgaggcta aagttagtgc gtttggtggc accttcctct ttatgttctg gcgcattgaa
     5341 ataagcgtcc cacgagccgt gccactcacc actgcctttt ttggtaatga tgttaaccac
     5401 gccgcccgcc gcgccgttgc cataacgcgc agctgccgga ccacgcagaa cttcaatacg
     5461 ttcaatcatt tcaggtggca cctaggaggt atcaccacgg gtatcgcgct cgccacgcca
     5521 gccctgacgc accgagttac ggctgcttac cggcttgccg tcaatcaaaa tcagcgtgtt
     5581 ttccggaccc ataccgcgaa tatcaatctg gcggttattc ccacgctgac cactggtgga
     5641 gttaccggtc aggttaacgc ctggcatggt acggatgatc tccgacacat cgcgggcaac
     5701 cgggtttttg cggatttcat ctgcggtgat ggtcgaaacg ccaggtgcct gcaagttctg
     5761 ctcggcggcg gtaacgacaa tggtatcgtc atgtgaaaca ggagtatcgg tcggctcttg
     5821 tgcctgcgct accccataaa tccccagatt gaccaacaag gccagggaat gaatcttctt
     5881 gttcattgtt ttattcctgc atttttgcca cgaattgcaa ctgtcgggca tggtcgtcat
     5941 caacacgacg catcccgcta ccgcgaaaac ctttgatcct gaaagacacg cagtgcagtt
     6001 ggttaattaa tgtccgcgct tcccacggcg cgccattacg ctattgcaaa tgcaaatagt
     6061 tatcaataat attatcaata tatttctgca atcaatgaaa aattgcacag taaacatggg
     6121 gttatggtgt gacggcgtta aaagtaggaa gtgagagctg gtggcagtcg aaacatggcc
     6181 cggaatggca gcgtctgaat gacgaaatgt ttgaggtcac tttccggtgg cgtgatcccc
     6241 agggttctga agaatactcg acgataaagc gcgtatgggt ctacatcact ggtgtgaccg
     6301 atcaccatca gaacagccag ccccagtcga tgcagcgaat tgcaggcacc aacgtctggc
     6361 agtggacgac acaacttaat gccaactggc gcggcagcta ctgttttatt cccaccgaac
     6421 gcgatgacat tttttctgca ccatcccccg atcgcctcga attacgtgaa ggctggcgaa
     6481 aactattacc ccaggcgata gccgatccgc tgaatccaca aagctggaaa ggcgggcgag
     6541 ggcacgctgt ttctgcactc gaaatgccgc aagcgcccct acaaccggga tgggattgtc
     6601 cgcaagcgcc agaaacacct gccaaagaaa ttatctggaa aagtgagcgg ttgaaaaatt
     6661 cacgccgtgt atggattttt accactggcg atgcaacagc aggagaacgt ccgctggcag
     6721 ttttgctcga tggcgaattt tgggcgcaga gtatgcctgt ctggcctgtg ctgacttcgt
     6781 tgacccatcg tcagcaactt cctccagccg tgtatgtgtt gatcgacgct attgacacca
     6841 cgcaccgcgc ccacgaactg ccgtgtaatg cggatttctg gctggcagta cagcaagagt
     6901 tattacccct ggtgaaagct attgcccctt ttagcgatcg cgccgatcgc accgtggtcg
     6961 ccgggcagag ttttggtggg ctttccgcgc tgtatgccgg actgcactgg cctgaacgct
     7021 ttggctgtgt attaagccag tcaggatcgt actggtggcc gcatcggggc gggcagcaag
     7081 aaggtgtttt acttgagcaa cttaaagctg gtcaagttag tgccgaaggt ctgcgcattg
     7141 tgctggaagc gggtattcgc gagccgatga tcatgcaggc caatcaggcg ctgtatgcgc
     7201 aattacaccc cataaaagaa tccattttct ggcgtcaggt tgacggcgga catgatgcgc
     7261 tttgttggcg cggtggcttg atgcaggggc taatcgacct ctggcaacca cttttccatg
     7321 acaggagttg aatatggcat tcagtaatcc cttcgatgat ccgcagggag cgttttacat
     7381 tttgcgcaat gcgcaggggc aattcagtct gtggccgcaa caatgcgcct taccggcagg
     7441 ctgggacgtt gtgtgtcagc cgcagtcaca ggtgtcctgc cagcagtggc tggaagccca
     7501 ctggcgtact ctgacaccgg cgaatttcac ccagttgcag gaggcacaat gagccagcat
     7561 ttacctttgg tcgccgcaca gcccggcatc tggatggcag aaaaactgtc ggatttaccc
     7621 tccgcctgga gcgtggcgca ttacgttgag ttaaccggag aggttgatgc gccattactg
     7681 gcccgcgcgg tggttgccgg attagcgcaa gcagatacgc tgcggatgcg ttttacggaa
     7741 gataacggcg aagtctggca gtgggtcgat gatgcgctga cgttcgaact gccagaaact
     7801 atcgacctgc gaaccaacat tgatccgcac ggtactgcgc agtcattaat gcaggcggat
     7861 ttgcaacaag atctgcgcgt cgatagcggt aaaccactgg tctttcatca gttgatacag
     7921 gtggcggata accgctggta ctggtatcag cgttatcacc atttgctggt cgatggcttc
     7981 agtttcccgg ccattacccg ccagatcgcc aatatttact gcgcatggct gcgtggcgaa
     8041 ccaacgcctg cttcgccgtt tacgcctttc gctgatgtag tggaagagta ccagcaatac
     8101 cgcgaaagcg aagcctggca gcgtgatgcg gcattctggg cggaacagcg tcgtcaactg
     8161 ccaccgcccg cgtcactttc tccggcacct ttaccggggc gcagcgcttc ggcagatatt
     8221 ttgcgcctga aactggaatt taccgacggg gaattccgcc agctggctac gcaactttca
     8281 ggtgtgcagc gtaccgattt agcccttgcg ctgacggctt tgtggctggg gcgattgtgc
     8341 aaccgcatgg actacgctgc cggatttatc tttatgcgtc gactgggctc ggcggcgctg
     8401 acggctaccg gacccgtgct caacgtttta ccgttgggta ttcacattgc ggcgcaagaa
     8461 acgctgccgc aactggcaac ccgactggca gcacaactga aaaaaatgcg ccgccatcaa
     8521 cgctacgacg ccgaacaaat tgtccgtgac agtgggcgag cggcaggaga ggaaccgctg
     8581 ttcggtccgg tactcaatat caaggtattt gattaccaac tggatattcc tggtgttcag
     8641 gcgcaaaccc ataccctggc aaccggtccg gttaatgacc ttgaactggc cctgttcccg
     8701 gatgaacacg gagatttgag tattgaaatc ctcgccaata aacagcgtta cgctgagcca
     8761 acgttaatcc agcatgctga acgcctgaaa atgctgattg cccagttcgc cgcggatcct
     8821 gcgctgttgt gcggcgatgt cgatattatg ctgccaggtg aatatgcgca gctggcgcag
     8881 atcaacgcca ctcaggttga gattccagaa accacgctga gcgcgctggt ggcagaacaa
     8941 gcggcaaaaa caccgcatgc tccggcgctg gcagatgcgc gttacctgtt cagctatcgg
     9001 gaaatgcgcg agcaggtggt ggcgctggcg aatctgctgc gtgagcgcgg cgttaaacca
     9061 ggcgacagcg tggcggtggc gctaccgcgc tcggtctttt tgactctggc gctacatgca
     9121 attgttgagg ctggtgcggc ctggttaccg ctggataccg gctatccgga cgatcgcctg
     9181 aaaatgatgc tggaagatgc gcgtccgtcg ctgttaatca ccaccgacga tcaactgccg
     9241 cgctttgccg atgttccaga tttaaccagc ctttgctata acgccccgct tacaccgcag
     9301 ggcagtgcgc cgctgaaact ttcacaaccg caccacacgg cttatatcat ctttacctct
     9361 ggctccaccg gcaggccgaa aggggtaatg gtcgggcaga cggctatcgt taaccgtctg
     9421 ctttggatgc aaaaccacta cccgcttaca ggcgaagatg tcgttgccca aaaaacgccg
     9481 tgcagttttg atgtctcggt gtgggagttt ttctggccgt ttattgccgg ggctaaactg
     9541 gtgatggctg aaccggaagc gcaccgcgac ccgctcgcta tgcagcaatt ctttgccgaa
     9601 tatggtgtaa cgaccacgca ctttgtgccg tcgatgctgg cggcatttgt tgcatcgctg
     9661 actccgcaaa ccgctcgcca gaattgcgcg acgttgaaac aggttttctg tagtggtgag
     9721 gccttacccg ctgatttatg ccgcgaatgg caacagttaa ccagcgtgcc gttgcataat
     9781 ctatatggcc cgacggaagc ggcggtagat gtcagctggt atccggcttt tggcgaggaa
     9841 ctggcacagg tgcgcggcag cagtgtgccg attggttatc cggtgtggaa tacgggcctg
     9901 cgtattcttg atgcgatgat gcatccggtg ccgccgggtg tggcgggaga tctctatctc
     9961 accggtattc aactggcgca ggggtatctt ggacgacccg atctgaccgc cagccgcttt
    10021 attgccgatc cttttgcccc tggcgaacgg atgtaccgta ccggagacgt tgcccgctgg
    10081 ctggataacg gcgcggtgga gtacctcggg cgcagtgacg atcagctaaa aattcgcggg
    10141 cagcgtatcg aactgggcga aatcgatcgc gtgatgcagg cgctgccgga tgtcgaacaa
    10201 gccgttaccc acgcctgtgt gattaaccag gcggcagcca ctggtggtga tgcgcgtcag
    10261 ttggtgggct atctggtgtc gcaatcgggc ctgccgttgg ataccagcgc attacaggct
    10321 cagcttcgcg aaacattgcc accacatatg gtgcccgtgg ttctgctgca acttccacag
    10381 ttacctctta gcgccaacgg caagctggat cgcaaagcct taccgttgcc ggaactgaaa
    10441 gcacaagcgc cggggcgtgc gccgaaagcg ggcagtgaaa cgattatcgc cgcggcattc
    10501 tcgtcgttgc tgggttgtga cgtgcaggat gccgatgctg atttctttgc gcttggcggt
    10561 cattcgctac tggcaatgaa actggcagcg cagttaagtc gacagtttgt tcgtcaggtg
    10621 acgccggggc aggtgatggt cgcgtcaact gtcgccaaac tggcaacgat tattgatggt
    10681 gaagaagaca gcacccagcg catgggattc gaaaccattc tgccgttgcg tgaaggtaat
    10741 ggcccgacgc tgttttgttt ccatccggcc tccggttttg cctggcagtt tagcgtgctc
    10801 tcgcgttatc tcgattcaca atggtcgatt atcggcattc agtcgccgcg ccctcatggc
    10861 cccatgcaga cggcggcaaa cctggatgaa gtctgcgaag cgcatctggc aacgttactt
    10921 gaacaacaac cgcacggtcc ttattacctg ctgggttatt cacttggcgg tacgctggcg
    10981 cagggcattg cggcgcgact gcgtgcccgt ggcgaacagg tggcatttct tggcttgctg
    11041 gatacctggc cgccagaaac gcaaaactgg caggaaaaag aagccaatgg tctggacccg
    11101 gaagtgctgg cggagattaa ccgcgagcgc gaggccttcc tggcggcaca gcagggaagt
    11161 acttcaacgg agttgtttac caccattgaa ggcaactacg ctgatgctgt gcgcctgctg
    11221 acgactgctc atagtgtacc gtttgacggt aaagcgacgc tgtttgttgc tgaacgcgcg
    11281 cttcaggaag gtatgaatcc cgaacgcgcc tggtcgccgt ggatagccga gctggatatc
    11341 tatcgtcagg attgtgcgca tgtggatatt atctctccag gggcgtttga aaaaattggg
    11401 ccgattattc gcgctac
//
LOCUS       DAZFLD010000152        11277 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.153, whole genome shotgun sequence.
ACCESSION   DAZFLD010000152 DAZFLD010000000
VERSION     DAZFLD010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11277
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.153"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            137..1489
                     /gene="celF"
                     /locus_tag="ACHKBL_003521"
                     /gene_synonym="chbF"
     CDS             137..1489
                     /gene="celF"
                     /locus_tag="ACHKBL_003521"
                     /gene_synonym="chbF"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFO5820339.1"
                     /translation="MSQKLKVVTIGGGSSYTPELLEGFIKRYHELPVSELWLVDVEGG
                     KAKLDIIFDLCQRMIDNAGVPMKLYKTLDRREALKDADFVTTQLRVGQLPARELDERI
                     PLSHGYLGQETNGAGGLFKGLRTIPVIFDIVKDVEELCPNAWVINFTNPAGMVTEAVY
                     RHTGFKRFIGVCNIPIGMKMFIRDVLMLKDSDDLSIDLFGLNHMVFIKDVLVNGKSRF
                     AELLDGVASGQLKASGVKNIFDLPFSEGLIRSMNLLPCSYLLYYFKQKEMLAIEMGEY
                     YKGGARAQVVQKVEKQLFELYKNPELKVKPKELEQRGGAYYSDAACEVINAIYNDKQA
                     EHYVNIPHHGHIDNIPADWAVEMTCTLGRDGATPHPRITHFDDKVMGLIHTIKGFEIA
                     ASNAALSGEFNDVLLALNLSPLVHSDRDAELLAREMILAHEKWLPNFADCIAELKKAH
                     "
     gene            1502..2260
                     /gene="chbG"
                     /locus_tag="ACHKBL_003522"
     CDS             1502..2260
                     /gene="chbG"
                     /locus_tag="ACHKBL_003522"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HFO5820340.1"
                     /translation="MERLLIVNADDFGLSKGQNYGIIEACRNGIVTSTTALVNGQAID
                     HAVQLSLDEPSLAIGMHFVLTMGKPLTAMPGLTRDGVLGKWIWQLAEEDALPLEEITQ
                     ELASQYLRFIELFGRKPTHLDSHHHVHMFPQIFPIVAKFAAEEGIALRIDRQPLSNDG
                     DLPANLRSSQGFSSAFYGEEISETLFLQVLDDSSHRGERSLEVMCHPAFIDNTIRQSA
                     YCFPRLTELDVLTSASLKGAIAQRGYRLGCYRDV"
     gene            complement(2518..4779)
                     /gene="katE"
                     /locus_tag="ACHKBL_003523"
     CDS             complement(2518..4779)
                     /gene="katE"
                     /locus_tag="ACHKBL_003523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase HPII"
                     /protein_id="HFO5820341.1"
                     /translation="MSQHNEKNPHQHQSPLHDSSEAKPGMDSLAPEDGSHRPAAEPTP
                     PGAQPTAPGSLKAPDTRNEKLNSLEDVRKGSENYALTTNQGVRIADDQNSLRAGSRGP
                     TLLEDFILREKITHFDHERIPERIVHARGSAAHGYFQPYKSLSDITKADFLSDPNKIT
                     PVFVRFSTVQGGAGSADTVRDIRGFATKFYTEEGIFDLVGNNTPIFFIQDAHKFPDFV
                     HAVKPEPHWAIPQGQSAHDTFWDYVSLQPETLHNVMWAMSDRGIPRSYRTMEGFGIHT
                     FRLINAEGKATFVRFHWKPLAGKASLVWDEAQKLTGRDPDFHRRELWEAIEAGDFPEY
                     ELGFQLIPEEDEFKFDFDLLDPTKLIPEELVPVQRVGKMVLNRNPDNFFAENEQAAFH
                     PGHIVPGLDFTNDPLLQGRLFSYTDTQISRLGGPNFHEIPINRPTCPYHNFQRDGMHR
                     MGIDTNPANYEPNSINDNWPRETPPGPKRGGFESYQERVEGNKVRERSPSFGEYYSHP
                     RLFWLSQTPFEQRHIVDGFSFELSKVVRPYIRERVVDQLAHIDLTLAQAVAKNLGIEL
                     TDDQLNITPPPDVNGLKKDPSLSLYAIPDGDVKGRVVAILLNDEVRSADLLAILKALK
                     AKGVHAKLLYSRMGEVTADDGTVLPIAATFAGAPSLTVDAVIVPCGNIADIADNGDAN
                     YYLMEAYKHLKPIALAGDARKFKATIKVADQGEEGIVEADSADGSFMDELLTLMAAHR
                     VWSRIPKIDKIPA"
     gene            4962..5225
                     /gene="cedA"
                     /locus_tag="ACHKBL_003524"
     CDS             4962..5225
                     /gene="cedA"
                     /locus_tag="ACHKBL_003524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division activator CedA"
                     /protein_id="HFO5820342.1"
                     /translation="MRLVKPVMKKPLRQQNRQIISYVPRTEPAPPEHAIKMDSFRDVW
                     MLRGKYVAFVLMGESFLRSPAFTVPESAQRWANQIRQENEVEE"
     gene            complement(5272..6663)
                     /gene="tcyP"
                     /locus_tag="ACHKBL_003525"
     CDS             complement(5272..6663)
                     /gene="tcyP"
                     /locus_tag="ACHKBL_003525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine/sulfocysteine:cation symporter"
                     /protein_id="HFO5820343.1"
                     /translation="MNFPLIANIVVFVVLLFALAQTRHKQWSLAKKVLVGLVMGVVFG
                     LALHTIYGSDSQVLKDSVQWFNIVGNGYVQLLQMIVMPLVFASILSAVARLHNASQLG
                     KISFLTIGTLLFTTLIAALVGVLVTNLFGLTAEGLVQGGAETARLNAIESNYVGKVSD
                     LSVPQLVLSFIPKNPFADLTGANPTSIISVVIFAAFLGVAALKLLKDDAPKGERVLTA
                     IDTLQSWVMKLVRLVMQLTPYGVLALMTKVVAGSNLQDIIKLGSFVVASYLGLLIMFA
                     VHGLLLGINGVSPLKYFRKVWPVLTFAFTSRSSAASIPLNVEAQTRRLGVPESIASFA
                     ASFGATIGQNGCAGLYPAMLAVMVAPTVGINPMDPMWIATLVGIVTVSSAGVAGVGGG
                     ATFAALIVLPAMGLPVTLVALLISVEPLIDMGRTALNVSGSMTAGTLTSQWLKQTDKA
                     ILDSEDDAELAHR"
     gene            complement(6796..7386)
                     /gene="ydjM"
                     /locus_tag="ACHKBL_003526"
     CDS             complement(6796..7386)
                     /gene="ydjM"
                     /locus_tag="ACHKBL_003526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310461.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFO5820344.1"
                     /translation="MTAEGHLLFSIACAVFAKNAELTPVLAQGDWWHIVPSAILTCLL
                     PDIDHPKSFLGQRLKWISKPIARAFGHRGFTHSLLAVFALLATFYLKVPESWFIPADA
                     LQGMVLGYLSHILADMLTPAGVPLLWPCRWRFRLPILVPQKGNQLERFICMALFVWSV
                     WMPHSLPENSAVRWSSQMINTLQIQFHRLIKHQVEY"
     gene            complement(7550..8218)
                     /gene="hxpB"
                     /locus_tag="ACHKBL_003527"
     CDS             complement(7550..8218)
                     /gene="hxpB"
                     /locus_tag="ACHKBL_003527"
                     /EC_number="3.1.3.22"
                     /EC_number="3.1.3.50"
                     /EC_number="3.1.3.68"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HFO5820345.1"
                     /translation="MSTPRQILAAIFDMDGLLIDSEPLWDRAELDVMASLGVDISRRN
                     ELPDTLGLRIDMVVDLWYARQPWNGPSRQEVVEQVIARAISLVEETRPLLPGVREAVA
                     LCKEQGLLVGLASASPLHMLEKVLTMFDLRDSFDALASAEKLPYSKPHPQVYIDCAAK
                     LGVDPLTCVALEDSVNGMIASKAARMRSIVVPAPEAQNDPRFVLANVKLSSLTELTAK
                     DLLG"
     gene            8365..8901
                     /gene="yniB"
                     /locus_tag="ACHKBL_003528"
     CDS             8365..8901
                     /gene="yniB"
                     /locus_tag="ACHKBL_003528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YniB family protein"
                     /protein_id="HFO5820346.1"
                     /translation="MTYQQAGRIAVLKRILGWVIFIPALISTLISLLKFMNTRQENQE
                     GINAVMLDFTHVMIDMMQANTPFLNLFWYNSPTPNFNGGVNVMFWVIFFLIFVGLALQ
                     DSGARMSRQARFLREGVEDQLILEKAKGEEGLTREQIESRIVVPHHTIFLQFFSLYIL
                     PVICIAAGYVFFSLLGFI"
     gene            complement(8942..9802)
                     /gene="yniA"
                     /locus_tag="ACHKBL_003529"
     CDS             complement(8942..9802)
                     /gene="yniA"
                     /locus_tag="ACHKBL_003529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HFO5820347.1"
                     /translation="MWQAISRLLSEQLGEGEIELRNELPGGEVHAAWHLRYAGHDFFV
                     KCDERELLPGFTAEADQLELLSRSKTVTVPKVWAVGADRDYSFLVMDYLPPRPLDAHS
                     AFILGQQIARLHQWSDQPQFGLDFDNALSTTPQPNTWQRRWSTFFAEQRIGWQLELAA
                     EKGIAFGNIDAIVEHIQQRLASHQPQPSLLHGDLWSGNCALGPDGPYIFDPACYWGDR
                     ECDLAMLPLHTEQPPQIYDGYQSVSPLPADFLERQPVYQLYTLLNRARLFGGQHLVIA
                     QQSLDRLLAA"
     gene            complement(9908..10198)
                     /gene="ghoS"
                     /locus_tag="ACHKBL_003530"
     CDS             complement(9908..10198)
                     /gene="ghoS"
                     /locus_tag="ACHKBL_003530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HFO5820348.1"
                     /translation="MASGDLVRYVITVMLHEDTLTEINELNNYLTRDGFLLTMTDDDG
                     NIHELGTNTFGLISTQSEEEIRELVSGLTQSATGKDPEIAITTWEEWNSNRK"
     gene            complement(10299..11228)
                     /gene="pfkB"
                     /locus_tag="ACHKBL_003531"
     CDS             complement(10299..11228)
                     /gene="pfkB"
                     /locus_tag="ACHKBL_003531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416237.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase II"
                     /protein_id="HFO5820349.1"
                     /translation="MVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINV
                     ARAIAHLGGSATAIFPVGGATGEHLVSLLADENVPVATVEAKDWTRQNLHVHVEASGE
                     QYRFVMPGAALNEDEFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISAAQNQG
                     IRCIVDSSGEALSAALAIGNIELVKPNQKELSALVNRELTQPDDVRKAAQEIVNSGKA
                     KRVVVSLGPQGALGVDSENCIQVVPPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMV
                     RFGVAAGSAATLNQGTRLCSHDDTQKIYAYLSR"
BASE COUNT         2826 a         2732 c         2890 g         2829 t
ORIGIN      
        1 tatcgtaaga aattgactga atttaatcag taagtcgtta tacctgacaa ttcacatatc
       61 tgtcctgttg ctggattatt tatgtccggg gggcagatat gccagatatc agtattctgt
      121 actgaaggga gaaattatga gccagaaatt aaaagtcgtc actattggtg gcgggagcag
      181 ctataccccg gagttactgg aaggatttat taagcgttat cacgaattgc cggtcagcga
      241 attatggctg gtggatgtcg aaggtggtaa agcgaaactg gatattattt ttgatctctg
      301 ccaacggatg attgataacg ctggcgtccc gatgaagctt tataaaacgc tggatcgccg
      361 cgaagcattg aaagatgctg atttcgttac tacccaactg cgcgttggcc aattaccggc
      421 gcgcgaactg gatgaacgta ttccattaag tcatggttat cttggtcagg aaactaacgg
      481 tgcgggcggt ctgtttaaag gtctgcgtac cattccggtg atttttgaca tcgtaaaaga
      541 tgtcgaagaa ctatgtccga atgcatgggt gattaacttc actaacccgg cgggaatggt
      601 cactgaagcc gtttatcgtc ataccggatt taaacgcttt atcggcgtgt gtaatattcc
      661 gatcggcatg aagatgttta ttcgcgatgt tctgatgctg aaagacagcg atgatttatc
      721 tatcgatctg ttcggcctca accatatggt gttcattaag gatgtgctgg taaatggcaa
      781 atcacgcttt gccgaattgc ttgatggtgt ggcatccggg cagttaaaag catctggcgt
      841 taaaaatatt ttcgatctgc catttagcga aggcttaatt cgttctatga atctgttgcc
      901 gtgttcttat ttgctttatt acttcaagca aaaagagatg ctggctattg aaatgggcga
      961 atactacaaa ggcggcgcac gagcgcaggt agtacagaaa gtcgagaaac aactttttga
     1021 gctatataaa aacccggagt tgaaagttaa gccgaaagaa ctggaacagc gcggtggggc
     1081 ttattactct gatgcagcgt gcgaagtgat caacgctatc tacaacgaca agcaagctga
     1141 acattacgtt aatatcccgc atcatgggca tattgataat attccggcag actgggcggt
     1201 agaaatgacc tgtacgctgg ggcgcgatgg cgcgacgcca catccgcgca ttacgcattt
     1261 cgatgataaa gtgatggggc tgattcacac cattaaaggc ttcgagattg ctgccagcaa
     1321 cgccgcactt agcggagagt ttaacgatgt gttactggcc ctaaacctta gtccgttggt
     1381 gcattccgat cgcgatgctg agctgctggc acgcgagatg attctggcgc acgagaaatg
     1441 gttgccaaat tttgccgact gcatcgcaga gcttaaaaaa gcacattaac cgaggctgat
     1501 tatggaacgc ttactgattg ttaatgccga tgattttggc ttaagcaaag gtcagaacta
     1561 cggcattatc gaggcctgtc gcaatgggat tgtcacatcg acgacggcgc tggtgaatgg
     1621 gcaggctatt gaccatgcgg tgcagttgag tcttgatgaa ccgagtctgg ccatagggat
     1681 gcactttgtc cttactatgg gcaagccact gacagctatg ccggggttaa ctcgcgatgg
     1741 tgtgctggga aaatggatct ggcagttggc agaagaagat gctttaccgc tggaagaaat
     1801 tactcaggag cttgccagtc agtatttgcg tttcattgag ttatttggac gcaaacctac
     1861 gcatcttgat agccatcatc atgtgcatat gttcccgcag attttcccga ttgtggcaaa
     1921 gtttgcggct gaagagggga ttgcgttgcg catcgaccgt cagccgctat ctaacgatgg
     1981 tgatttaccg gctaatctgc gcagttcgca gggattcagt agcgcgtttt atggtgaaga
     2041 gattagcgag acactgttcc tacaagtgct tgatgactcc agtcatcgcg gcgagcgctc
     2101 tctggaagtg atgtgccacc cggcgtttat cgataatacg attcgccaga gtgcttattg
     2161 cttcccgcgg ttaacggagc tggatgtgtt gacatcagcg tcgttgaaag gtgctatcgc
     2221 tcagcgtggt tatcggttgg ggtgttatcg ggatgtgtaa gtgaggatgt ttgccagatg
     2281 cggcgtgaac cttgcatccg gcgattaatt cctacaaagg cggcgtaact gcgccgcttg
     2341 agactgctga caaacgcaaa actgcctgat gcgcttcgct tatcaggcct acgtttttcc
     2401 tgcaatatat tgaatttgca cagttttgta ggccggataa ggcgttcacg ccgcatccga
     2461 cataaacaaa gcgcactatg taaatcattt gaggcggcgc aactgcgccg cctcccatca
     2521 ggcaggaatt ttgtcaatct taggaatacg tgaccacacg cggtgtgctg ccatcagcgt
     2581 taacagttca tccataaaac taccgtcggc gctgtcagct tccacaatcc cttcttcacc
     2641 ctggtcagcg accttgattg ttgctttaaa cttgcgcgcg tctcctgcca gcgcaatcgg
     2701 tttaaggtgt ttgtaggctt ccatcaggta gtagttggca tcgccgttgt cagcgatatc
     2761 cgcgatattg ccgcaaggga caataaccgc atcgaccgtc agcgaaggtg caccggcaaa
     2821 ggtagcggct ataggcaaca ccgtaccgtc atccgcagtc acttcaccca ttcgggagta
     2881 gagcagtttg gcatgaacgc ctttggcctt cagcgccttg agaatggcca gaaggtctgc
     2941 cgatctcact tcatcattaa gtaaaatcgc taccacgcga cctttcacat caccgtcagg
     3001 aatggcgtac aaacttaagg atggatcctt tttcagaccg ttgacgtccg gaggcggggt
     3061 gatattcagc tggtcgtccg tcagctcgat accgagattt ttcgccaccg cctgggccag
     3121 agtgagatca atatgtgcca gctggtcaac aacgcgctca cgaatatacg gacgaacgac
     3181 tttgcttaac tcaaaactga aaccatcgac aatatggcgc tgctcgaatg gcgtctgact
     3241 tagccagaac agacgcggat gggaataata ttcgccaaac gatgggctgc gctcgcgaac
     3301 tttattgcct tccacgcgct cctggtatga ttcaaaaccg ccgcgtttcg gccccggcgg
     3361 tgtttcgcgc ggccagttat cgttgatcga gttcggttca taattcgccg ggttagtgtc
     3421 gatccccata cgatgcatgc cgtcacgctg gaaattatgg taagggcagg tcgggcggtt
     3481 aatcggaatc tcatggaaat tcggcccacc aagacgactg atttgtgtat cggtatagga
     3541 gaacaaacgt ccctgcaaca ggggatcgtt ggtgaagtcc agaccaggaa caatatgacc
     3601 tggatggaat gctgcttgtt cgttttcggc aaagaagtta tccggattgc gattgagcac
     3661 catcttgccg acacgctgaa cgggcaccag ttcttccggg ataagtttag ttggatcaag
     3721 aaggtcgaag tcgaacttaa attcatcttc ctcaggaatc aactggaagc ccagttcgta
     3781 ttccggaaaa tcgcctgctt cgatggcttc ccacaactcg cggcggtgga agtccgggtc
     3841 acgtccggtt agtttttgtg cttcatccca aacgagtgag gctttacctg ccagtggttt
     3901 ccagtggaaa cgtacgaacg ttgccttccc ttcggcatta atcaggcgga aggtgtgaat
     3961 accgaagcct tccatggtgc ggtaactgcg cgggatcccg cgatccgaca tcgcccacat
     4021 cacgttgtgc agagtttcag gttgcagaga aacataatcc cagaaagtat cgtgggcgct
     4081 ttgcccttgt ggaattgccc agtgcggttc tggttttacc gcatgaacaa aatcggggaa
     4141 tttatgcgca tcctggataa agaagattgg cgtgttattg ccaacgaggt caaaaatacc
     4201 ctcttcggta tagaacttgg tggcaaagcc acggatatca cgcacggtat cggcagagcc
     4261 agcaccaccc tgaacggtag agaaacgtac aaatactggg gtgattttgt tcggatctga
     4321 gaggaaatct gctttggtga tatcgcttaa gcttttatat ggctggaaat aaccgtgagc
     4381 tgctgatccg cgtgcatgaa caatacgttc cgggatgcgc tcatggtcaa agtgagtgat
     4441 tttctcgcgc agaataaaat cttccagcag agttggacca cggctaccgg cacgcagtga
     4501 gttttgatcg tcggcgatgc gcacgccctg attagtggtc agcgcataat tttcactgcc
     4561 tttgcgtacg tcttccagag aattaagttt ttcgttacgc gtatcagggg ctttcaggct
     4621 ccctggggcg gtaggttgtg caccaggcgg tgttggttca gccgctggac gatgagatcc
     4681 gtcttcaggt gccagtgagt ccatccccgg tttcgcttcg ctggaatcgt gtagtggtga
     4741 ctggtgctga tgtgggttct tttcgttatg ttgcgacatt gaactcgtct ccttaaatta
     4801 ttactgaaag ggccgctgtt ttggtcaaaa aaccctctaa ctatagacca tcagccagat
     4861 cccgcttcgg agactattct ggaaacggtg ttatgaaata cacgcaaagc gggacgtaca
     4921 ggggctaaat cggctaaact acagtttttc tgatttttaa tatgcgttta gtgaagccag
     4981 taatgaagaa accgctccgc cagcaaaacc gccagattat tagctatgtc ccacgcacgg
     5041 aacccgcgcc gccagaacat gcgataaaga tggattcgtt tcgtgatgtc tggatgctgc
     5101 gtggcaaata tgttgcgttt gtactgatgg gagagtcatt tctgcgctca ccggcgttta
     5161 ctgtgcctga atccgcccag cgttgggcaa atcagattcg tcaggaaaat gaggtcgaag
     5221 aataaaaaaa cacctgtcgg gatgacaggc gtttaacgta acaaatcagg attaacggtg
     5281 tgccagttcg gcgtcgtctt cgctatccag aatagcttta tcggtttgct tcagccactg
     5341 gctggtcagc gtgccagcgg tcatcgagcc actaacgttt agcgccgtac gacccatgtc
     5401 gataagcggt tcaacggaga ttaacaatgc caccagcgtt accggcaggc ccatcgcagg
     5461 cagcacaatc agtgcggcga aagttgcacc accaccgaca ccggcaacgc ctgcggaact
     5521 aacggtaaca ataccgacca gcgtcgcaat ccacatcggg tccatcgggt taatgccaac
     5581 cgtaggcgca accatcaccg ccagcatcgc cggatacaaa ccggcacagc cgttctgacc
     5641 aatggttgca ccgaaagagg cggcgaaact ggcgatggat tcaggaacac cgagtcgacg
     5701 cgtttgtgct tccacattca gtgggataga cgcagcactg gaacggctgg taaaggcaaa
     5761 cgtcagtact ggccatacct tacggaagta cttcagcggg ctcacgccat taatacccag
     5821 cagaaggcca tgcactgcaa acataatcag aagaccgagg taggacgcga caacgaaact
     5881 tcccagtttg atgatgtctt gcaggttaga acctgcaacc actttggtca tcagcgccag
     5941 aacgccgtaa ggggtcagct gcatgaccag gcgaaccagt ttcatcaccc agctttgcag
     6001 ggtatcgata gcggttaaga cgcgttcacc tttcggcgca tcatccttca gcagcttcag
     6061 cgcagctacg ccgaggaatg cggcaaaaat taccacgctg ataattgacg tcggattggc
     6121 tccggtcaaa tcggcaaacg ggtttttcgg gataaaggac aagaccagct gcggaacgct
     6181 caggtcagag actttaccaa catagttact ttcgatggcg ttcaaacgtg cagtttctgc
     6241 accaccctga accagacctt cagccgtcaa accaaacagg ttggtgacca gcacaccgac
     6301 cagcgccgca atcagtgtgg taaacaaaag cgttccgatg gtcagaaaac tgattttgcc
     6361 taactgtgat gcgttatgca gacgggcaac cgcgctcaga atagaggcaa agactaacgg
     6421 cataacgatc atttgcagga gttgaacata gccgttacca acaatgttaa accactgtac
     6481 agaatcttta agtacctggc tgtcagaacc ataaatggta tgcagggcga ggccaaaaac
     6541 cacacccatc accagaccca ccagcacttt tttcgccaga ctccactgtt tatggcgggt
     6601 ctgagccagc gcaaacagca gtacaacgaa caccacgatg ttcgcaatta atggaaagtt
     6661 catccccgtt ctcctgatct tattatttct atcactgcgt agcagtcata gtgttggcgc
     6721 aagattaaca gaactgtgag ctggctctta tattcaaatg gaattgctta ttccagaaag
     6781 gtgatttttg cctttttagt attcaacctg atgcttaata agccgatgaa actggatctg
     6841 caaggtattg atcatttgcg atgaccaacg gacagcgctg ttttcgggta atgaatgggg
     6901 catccatacc gaccagacaa ataatgccat gcagataaaa cgttccagtt ggttgccctt
     6961 ttggggaacc aggataggca agcggaaacg ccagcggcat ggccagagca ggggaacacc
     7021 ggcgggtgtc agcatatcgg caagtatgtg gctcaaataa cccagcacca ttccttgtag
     7081 cgcatcagcc ggaatgaacc agctttcagg aaccttaagg taaaaggttg ccagcagcgc
     7141 aaataccgcc agcagactgt gggtaaaacc acgatgccca aaagcgcggg cgattggttt
     7201 tgatatccac tttaatcgct gcccaagaaa cgactttggg tgatcgatgt ccggtaacaa
     7261 acacgtcagg attgcggaag ggacaatatg ccaccagtca ccctgtgcca gcacgggcgt
     7321 cagctcggca tttttggcaa ataccgcaca agcaatagaa aagagaaggt gaccttccgc
     7381 cgtcatgata aaactcacta aactgtcgat acgtacagta tagggttttt atacagtgca
     7441 cggaagtggt gacggtgtaa ctctttctct taaattggcg attaatccgt cagttgaatg
     7501 caaattcaga taaagaacca ggggcagtgt gatcactgcc ccctggtcat taaccgagaa
     7561 ggtcttttgc ggtgagttct gtcagcgatg agagtttgac gtttgctaat acaaaacgtg
     7621 gatcattttg cgcttctggc gcaggaacga cgatggaacg catgcgggct gctttagagg
     7681 cgatcatgcc atttaccgaa tcttccagtg ccacgcaggt cagagggtca acccccagtt
     7741 ttgctgcgca gtcgatatat acttgcggat gcggcttgct gtaaggcaat ttttcagccg
     7801 aggcgagggc atcgaaactg tcgcgtaaat caaacatggt caatactttt tccagcatat
     7861 gcagcggtga cgcggaggcc agccccacca ataaaccttg ttctttgcat aacgcaacgg
     7921 cttcacgcac acctggtaat aatggacgcg tctcttcaac cagtgaaatg gcacgagcaa
     7981 taacttgttc tactacttcc tgacggcttg gcccattcca tggttgccgg gcgtaccaaa
     8041 gatcgaccac catatcgatg cgtaaaccta aggtgtccgg cagctcgtta cgacgggaga
     8101 tatccacccc caggcttgcc atcacatcca gttcggctcg atcccataaa ggttctgagt
     8161 cgataagtaa tccatccata tcaaaaattg cagcaagaat ctgacgcggg gttgacatta
     8221 caacctctcc tttagagcat tatatgtaag gtattaagcc gtgcttatga ttaagcgcct
     8281 atcatatcgc ctttaaagaa tgggcgaaaa cagatatcta caggtaaact aagtgaaacg
     8341 acgcgtctta atcaaggaga actcatgacg tatcaacaag ctggacgcat tgctgttttg
     8401 aaaaggattt tgggttgggt gatttttata cctgcattga tctctacatt gatttctttg
     8461 cttaaattta tgaatacccg gcaggaaaac caggaaggca ttaatgcggt catgctcgac
     8521 tttactcatg tcatgatcga tatgatgcag gcgaatacgc cttttttgaa tctgttctgg
     8581 tataactccc cgacgcccaa ttttaatggt ggcgtgaacg ttatgttctg ggtgattttc
     8641 ttcctgattt ttgtcggact ggcgttgcag gattccggtg cccggatgag tcgccaggcg
     8701 cgcttcttgc gggaaggtgt tgaagatcaa ctcattctgg aaaaagccaa aggggaagag
     8761 gggctaacgc gtgaacaaat tgagtcacgt attgttgtgc cgcaccatac tattttcctg
     8821 cagtttttct cgctgtatat cctgccggtt atttgtattg ctgcaggtta tgtgttcttt
     8881 tctctgctgg ggtttattta atcaaaaggc cgcacggagc ggccattatc ctcaccccat
     8941 attatgctgc taataatcta tccagtgact gctgagcaat aaccaaatgc tgaccgccaa
     9001 ataaccttgc acgatttaac agcgtgtaga gttggtaaac cggttgacgt tcaaggaaat
     9061 cggcaggtag cggggatact gactgatagc cgtcatagat ttgtggcggc tgttcagtat
     9121 gcagcggtaa catcgccagg tcgcactctc ggtcacccca gtagcaggcc gggtcgaaaa
     9181 tgtacgggcc atccggaccc agcgcacagt tgccggacca taaatcgccg tgcaacagag
     9241 aaggctgcgg ttgatgcgag gccagacgct gctgaatatg ctcgacgatg gcatcgatat
     9301 tgccgaaagc gatccctttt tctgccgcca gttccagctg ccagccaatc cgttgttcag
     9361 caaaaaaagt tgaccagcga cgttgccagg tgttgggctg gggagttgtg gagagcgcgt
     9421 tatcgaaatc gaggccaaat tgcggttggt cgctccattg atgtaaacgc gcaatttgct
     9481 gaccaagaat aaatgcgcta tgcgcatcca gcgggcgagg tgggagataa tccatcacca
     9541 gaaaactgta gtcacggtca gcgccaactg cccaaacctt aggcacggtg acggttttac
     9601 tacgcgacag taactccagt tggtcggctt cggcggtaaa accgggaagc agttcccttt
     9661 catcacattt gacgaaaaag tcatgtcctg catagcgcaa atgccatgcg gcatggactt
     9721 ctccgccagg cagttcatta cgcagttcga tttcgccttc acctaactgc tcgctcaaaa
     9781 gacgactgat tgcctgccac atgatgtctc tcccatgttg tctgccagat cataaggtta
     9841 gcgcaagaat gcggtgtaaa aacacgagct aacgcacacc acagactgat tattgcccaa
     9901 aaaccattta ttttctgttg ctattccatt cctcccaggt cgtgatggct atttcaggat
     9961 ctttaccggt tgcactttgg gtaagccccg aaacaagttc tctaatttct tcttcacttt
    10021 gggtactgat aagtccaaaa gtattagtcc ccagctcatg gatatttcca tcatcatccg
    10081 tcatggtgag caaaaaaccg tcgcgagtca ggtaattatt caactcgtta atttcagtca
    10141 atgtatcttc gtgcaacatg acggttatga cataacggac aagatcgcca ctggccatag
    10201 ttcacccctt tgttatcaca tgacttttga gcatagtcgg agaaacgcgt tgccgacagg
    10261 ttggtggtga ttcccccaat gctgggggaa tgtttcagtt agcgggaaag gtaagcgtaa
    10321 attttttgtg tatcgtcatg ggagcacagg cgtgttccct gattgagtgt ggctgcactc
    10381 cccgcagcta cgccaaaacg gaccatctct tcaagagagg cattttctgc cagtttcagt
    10441 gtcatcgcgc cgaccatgct gtcaccagcg ccaacggtgc tctggctttt caccggtggt
    10501 ggcacaacct gaatgcagtt ttcactatca acgcccagcg ccccttgtgg acccagggaa
    10561 acgacaactc gtttggcctt gccgctatta acgatttcct gcgcggcttt gcggacatcg
    10621 tctggctggg tgagttcgcg attcaccagc gcactgagtt ctttttggtt aggcttaacc
    10681 aactcgatgt taccaatcgc cagtgctgca cttaacgctt cgccagaact gtcgacgata
    10741 cagcggatcc cttggttttg cgcagcggaa atcagttggg ttaatttttc cagcttcaca
    10801 cctggcggca ggcttccgct tatgaccagg atagcaccgg attcaatttc cagaacttgc
    10861 tcttcaagct ggcgaaactc atcttcattt aatgccgcgc ctggcatgac aaaacgatac
    10921 tgctcaccgc ttgcttccac atgtacgtgt aaattctgcc gggtccagtc tttggcttct
    10981 acagtagcga cggggacatt ttcatctgcc aacagtgaaa ccagatgttc gccggtcgca
    11041 cctcccaccg ggaagatcgc tgtagcactg cctccaagat gggcaatggc gcgggcgacg
    11101 ttgatgccgc cgcctccggg ttcgaatacc ggtgcggtac agcgcagctt tccttcggga
    11161 taaatttgcg gggtaattgt tgcgctatcg agagagggcg caagtgtcaa cgtatagata
    11221 cgtaccatca tttcctccta taggctgatt tcagtctggc accgaatcag cggaaag
//
LOCUS       DAZFLD010000153        11228 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.154, whole genome shotgun sequence.
ACCESSION   DAZFLD010000153 DAZFLD010000000
VERSION     DAZFLD010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11228
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.154"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..532
                     /locus_tag="ACHKBL_003532"
     CDS             <1..532
                     /locus_tag="ACHKBL_003532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5820350.1"
                     /translation="ERYTIQINNAYNTIQSEKDDIESRIEKLENSVVELNKKLSVQIP
                     SEGEKKRRDEQFAAFYDYCRKVMSRNLAECFRIQKNRERSANSEILDNFLKGTHLASD
                     CFIAHSFGGFHEAIKHDVERSPGILCSIPVSIEIDTNTITGELVTAGVGIFRHEGNDA
                     KGSFTKIGPFFLKVER"
     gene            1044..1577
                     /locus_tag="ACHKBL_003533"
     CDS             1044..1577
                     /locus_tag="ACHKBL_003533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFO5820351.1"
                     /translation="MNTKIKYGLSAAVLALIAAGAPAPDILDQFLDEKEGNHTTAYRD
                     GAGIWTICRGAIMVDGKPVIPGMKLSKEKCDWVNAIERDKALAWVEKNIRVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRINAGDRRGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     ISRRDQESALACWGIDR"
     gene            1851..2546
                     /locus_tag="ACHKBL_003534"
     CDS             1851..2546
                     /locus_tag="ACHKBL_003534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000661712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HFO5820352.1"
                     /translation="MKEMISVDREISMSSLDFLNNIINPARAEAGEVPHEPRKFLAKI
                     EDELELDGTGKKFRLNNNQTRTAYYDLDFDQMMLVGMRESKAVRRSVLARLKAMHGIQ
                     LPQTLPEALRFAAKLAEQKAELENQLAIAAPKAEFVDNYVEASGLMGFREVAKLLGIK
                     ETDFRLFLLENGIMYRLAGKMTPYSHHLDAGRFSVKTGEAGNGHAFTQVKFTPKGVQW
                     IAGLLAAWRATAA"
     gene            2641..2772
                     /locus_tag="ACHKBL_003535"
     CDS             2641..2772
                     /locus_tag="ACHKBL_003535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5820353.1"
                     /translation="MSDKLIMLAKGLCVIVGISFSLMLVALFLSITWMALTSAGLVG"
     gene            2995..3180
                     /locus_tag="ACHKBL_003536"
     CDS             2995..3180
                     /locus_tag="ACHKBL_003536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rz1 family lipoprotein"
                     /protein_id="HFO5820354.1"
                     /translation="MRELKMKLCVLMLPLVVSACGSTPPATVPCVKPPAPPAWIMQPA
                     PDWQTPLNGIISSSENG"
     gene            3266..3490
                     /locus_tag="ACHKBL_003537"
     CDS             3266..3490
                     /locus_tag="ACHKBL_003537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5820355.1"
                     /translation="MKKLLVTVEPFQGTIPFRILQRGRVLVEGSFSGKCTQLHSRTFQ
                     VNATNEELTVECTMNAAKCRMVSAALQPVC"
     gene            3835..4161
                     /locus_tag="ACHKBL_003538"
     CDS             3835..4161
                     /locus_tag="ACHKBL_003538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HFO5820356.1"
                     /translation="MKRNLPLIILLSSLVMGCTQHKTDMPRQLVKALPQYPAYAAANY
                     IKGRVDVKFDIGADGTVTRIEFIRSEPHHLFDEQVVKAMAKWRFEKDRPRKGVKKTFI
                     FSPSAP"
     gene            complement(4293..4493)
                     /locus_tag="ACHKBL_003539"
     CDS             complement(4293..4493)
                     /locus_tag="ACHKBL_003539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000095744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HFO5820357.1"
                     /translation="MSSKNRTRRTTTRNIRFPNHMIEQINIALQHKGSGNFSAWVIEA
                     CRRRLATDATHLRPASMTNNEK"
     gene            4535..4900
                     /locus_tag="ACHKBL_003540"
     CDS             4535..4900
                     /locus_tag="ACHKBL_003540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFO5820358.1"
                     /translation="MAELRTLQSRIKTLNTRRVNILKGEQRRVSGSARVSLKRHIWLR
                     DAGQCCLCGRVVDLCDSELDHRIALQFGGGNEETNLWTLCTECHRQKSAREAAGGMPD
                     PTLPEVSGGSGRADDIIGL"
     gene            5183..5740
                     /locus_tag="ACHKBL_003541"
     CDS             5183..5740
                     /locus_tag="ACHKBL_003541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HFO5820359.1"
                     /translation="MLTTQKRKFALALMSGKNRTAAAIEAGYSEKSARCKGSQLAKDP
                     EVLAFIARKQGETVEVDEVPVYRQKKTQACHREVPPVEKKNQVSVPPDDGQSFSPLSG
                     IDYMEDGLPDPVKAMGRILVENISIDPKLALDAAWRLAQFTHHKKGDAGKKSAKGDAA
                     KKAANRFAVPPPPRLVVNNDNEGNG"
     gene            5737..7398
                     /locus_tag="ACHKBL_003542"
     CDS             5737..7398
                     /locus_tag="ACHKBL_003542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HFO5820360.1"
                     /translation="MIPVWSTACPDWAERLKKGLSIIPAPIYPDQAAHALAIFKQLRI
                     VDAPGSPTFGESCAPWVFDLVAALFGSYDAQTGVRHIKEVFILIPKKNSKSTLAAGIM
                     MTALLLNWRQAAGYTILAPTVEVAANAFNPARDMVRRDDDLDDLCQVQTHIRTITHRV
                     TDTTLKVVAADPNTVSGIKSVGTLIDELWLFGKQYKAEDMLREAIGGLASRPEGFVVY
                     TTTQSNEPPAGVFRQKLQYARDVRDGKINDPHFLPVIFEHPPEMVERGEHLLMENLAM
                     VNPNLGYSVDEAFLYREYRKAREAGEEAFRGFMSKHANVEIGLALRSDRWAGADFWEQ
                     QGRRVSLDDILQRADVVTVGIDGGGLDDLLGMYVIGRDRETREWLGWGHAWAHETAVV
                     RRKSEASRFQDFVASGDMTIVRRVGDDTAEVAEYVRRIHEAELLDHIGIDPSGVGQIL
                     DSLAEAGIPDGIVVGISQGWKLGGAIKTTERKLAEGVLVHGGQPLMAWCVGNARVEPK
                     GNAILITKQASGRGKIDPLMALFNAVSLMSLNPEPKKKEYAVFFI"
     gene            7462..9399
                     /locus_tag="ACHKBL_003543"
     CDS             7462..9399
                     /locus_tag="ACHKBL_003543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFO5820361.1"
                     /translation="MTLKRACSLLTVKSFSEDERVITGIASTPSPDRDGDILEPEGAE
                     FGSAIPFLWQHDHSRPVGQCTVRRVSEGLEITATLVKPVPDMPSQLAARLDEVWAAIK
                     TGLVRGLSVGFRPHEYTFLDGGGLHFLRWELMEVSAVTVPANAECTIRTIKSYDRPFS
                     AASGNRKPVVKIASSAGAAAQSTTVFHKEKTIMNIGEQIKSFENKRAALAASLEEVMT
                     KAAEEGRTLDVEEEEHYDNTAAEIRQVDAHLKRLRELEAGKAATAQPVKQAGNGNVTA
                     VASAPVIRVEQKLDKGIGFARFAKSLAAAKGVRSEALEVARRQYPDDSRLHHVLKSAV
                     GAGTTTDPQWAGSLSEYQEYAQDFIDYLRPQTIIGRFGQGGIPALRQVPFNIRVHAQV
                     SGGAAGWVGEGKAKPLTKFDFESITFSHAKVSAIAVLTEELIRFSSPAADALVRNALA
                     EAVVARLDTDFVDPKKAAVADVSPASITHDVKGTASTGNPDADAEAAFGQFVAANLQP
                     TGAVWLMSSTNALALSMRKNALGQKEYPDMTLLGGSFQGLPVIVSQYVGDQLVLVNAP
                     DIYLADDGGVAVDMSREASLEMQSEPTGDSTTPSPVELVSMFQTGSVAIRAERWINWR
                     RRRTAAVAVITGVNYGSASGG"
     gene            9444..9665
                     /locus_tag="ACHKBL_003544"
     CDS             9444..9665
                     /locus_tag="ACHKBL_003544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5820362.1"
                     /translation="MAKIRYLQGTHDARAGDIRDVAQPCAEVLVRLGKAEYITVRRPA
                     GQKKKRDAEHGECGTFYGEPEKTRNQDVT"
     gene            9611..11228
                     /locus_tag="ACHKBL_003545"
                     /pseudo
     CDS             9611..11228
                     /locus_tag="ACHKBL_003545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309571.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
BASE COUNT         2673 a         2631 c         3335 g         2589 t
ORIGIN      
        1 agagcggtat accatacaaa ttaacaatgc atataacacg atacagtcag aaaaagatga
       61 tatcgaatca cgcattgaaa aactggagaa cagcgtcgtt gaactgaata aaaaactgtc
      121 ggtgcagatc ccttccgaag gtgaaaaaaa acgccgtgat gagcagttcg cggcgtttta
      181 cgattattgc cggaaagtga tgagcagaaa tctcgcagag tgtttccgta ttcaaaaaaa
      241 cagagagcgc tctgcaaata gtgagattct ggataatttt ttaaagggga cgcatctcgc
      301 cagtgattgt ttcattgctc attcttttgg tggctttcat gaggccatca aacatgatgt
      361 tgagagaagt ccaggtatcc tctgttccat tcccgtaagc atcgaaatag acacaaatac
      421 gataaccggt gaactcgtta ccgccggagt cggtatattc cgtcatgagg ggaacgatgc
      481 caaaggttcc tttacaaaaa tcgggccgtt ctttctgaag gtagagcgat agtttgttaa
      541 gtacccatcc gacagctcgc tcattttggt ttattttcgt atcgcgaaac aaaaattcga
      601 agcggccaga tgacgcatgg gtgcaaccaa ataccggggc gtattcatca acctttggtg
      661 ttcctgaaaa catgcagtcc gtgctctcga atgcagatga attgctgttg atccacttaa
      721 caaaagaaaa aaaggtctga atcgcttgtc tgtctgaata ttcaacaaaa tcctcaaggt
      781 gtgtctcgat cagttccggg tgtttacgaa agtcataata ttgaccggga cgatatttat
      841 gagtggttgt tttatcccat ggttgtgctc tgcgtccctg taaacggagg tctgccgctg
      901 gatagataag cataagtaat ctcctggtgg aatgtaatcc acgatcatcc ggggaaatta
      961 agaacccgcc agtgcccacc actggtgggg tgaaggctta acatatccag ggattcggaa
     1021 ccgataaatc ctgataaata tccatgaaca ccaaaatcaa atacggcctg tcggctgccg
     1081 ttctggcgct gattgccgca ggggcgcctg cgcctgatat tctcgaccag tttctggatg
     1141 aaaaagaagg caaccacacc acagcatacc gtgatggtgc gggtatctgg accatctgcc
     1201 gaggtgccat catggtggat ggtaagcctg tgattcctgg catgaagctg tcgaaggaaa
     1261 aatgcgactg ggttaacgct atcgaacggg ataaggcgct ggcgtgggtg gagaaaaaca
     1321 tcagagtgcc actgaccgaa ccccagaaag cggggatcgc gtcattctgc ccgtataaca
     1381 ttggtcccgg taagtgtttc ccgtcgacgt tttataaacg aattaatgca ggcgatcgca
     1441 ggggagcgtg tgaggcgatt cgctggtgga ttaaggacgg tggcagagac tgccgtattc
     1501 gctcaaataa ttgctacggt cagatatccc gtcgtgacca ggagagtgcg ctggcgtgct
     1561 ggggaatcga cagataagca gaatattttg ctgaaaaatg acgttggtca acgcgggtgg
     1621 ataacacgaa atcctgcgaa ctggcaaaac ctaagtgaat aaagtcagga acattgtttc
     1681 acgcagaggc accgtaatgg tgcctttgtc atttctgcgc ttcgcacaag cgtaaataaa
     1741 ccaaagaacc tttcaggatg agccctggtg gataaccggc agtggtctgg ttaaccctct
     1801 ttgggctggt tattcctgtg cgcagggttc atcactaaaa ggaatcaacc atgaaagaga
     1861 tgatttctgt cgatcgtgaa atatccatga gtagtctgga ttttctgaat aacattatta
     1921 atccagcccg ggcagaagcc ggagaagtcc ctcatgaacc gcgtaagttt cttgcaaaaa
     1981 ttgaggatga gctagagctt gatggaaccg gaaaaaaatt ccggttaaac aataaccaga
     2041 caagaacggc atactatgat ctggattttg accagatgat gctcgttggc atgagagagt
     2101 caaaggccgt tcgtcgttct gtgctggcaa ggctcaaagc gatgcatggt attcagctcc
     2161 cccagacttt acctgaggcg ttgcgatttg cggcaaaact ggcagaacag aaagcagagc
     2221 tggaaaatca actggcaata gcagcaccga aggctgaatt tgttgataac tatgttgaag
     2281 catctggtct gatgggattc cgggaagttg ctaagttact cggtatcaaa gaaaccgatt
     2341 tccggctgtt tttgttggag aacggaataa tgtatcgcct cgctggaaaa atgacgcctt
     2401 actcgcatca cctggatgcg gggcggttta gcgtgaaaac gggcgaggcg ggcaatggtc
     2461 atgcttttac gcaggttaaa ttcaccccaa aaggtgttca gtggattgct ggtctgcttg
     2521 ctgcatggag agctaccgca gcatgaagat gataaaaatg gactggaaat ttttgctggt
     2581 ctggctgatt ccgtttttat gggtggttgc ccggttaatt actgctatta agaggtaaag
     2641 atgtcagaca aactcataat gctggcgaag ggcctttgtg taatcgtcgg tatttcattt
     2701 tcactaatgc tggttgctct ttttctttcc ataacctgga tggcgttgac ttcggcaggg
     2761 ctggtggggt gagcataaac cgaatgcttt ccgcgtttac cgttattctg ctggtggtct
     2821 gtggtgcgct tagtctgggg ctgaatcatt accgcgatag cgccatcacc tacaaagcgc
     2881 agcgcgataa aaaagccaga gagctggagc tggcaaacgc aaccattact gatatgcagg
     2941 tgcgccagct cgatgttgct gcgctcgatg caaaatactc gagggaatta gccgatgcga
     3001 gagctgaaaa tgaaactctg cgtgctgatg ttgccgctgg tcgtaagcgc ctgcggatca
     3061 acgccacctg ccacggtacc gtgcgtgaag ccaccagcac ctccggcgtg gataatgcaa
     3121 ccggcccccg actggcagac accgctgaac gggattattt catcctcaga gaacggttga
     3181 tgacaatgca gaagcagctg gaaggggcga aggaatatat ccgcactcag tgtattaact
     3241 agtatttttg ttatccggag aatgcatgaa gaaattactg gtaaccgtag aaccttttca
     3301 gggaacaatt ccgttccgta ttttgcagcg tggtcgtgtt cttgttgaag gttcgttcag
     3361 tggtaaatgt acgcaattac actcccggac ctttcaggtg aatgccacga atgaagagct
     3421 aaccgttgag tgtacgatga atgccgctaa atgccgcatg gtatccgctg cattacagcc
     3481 agtgtgttga gcgaccttat tatccatgcg cggtattgtc gccgtattcc cgtattaaca
     3541 gagaccgcag cccgacaggg agactcctct gcgcgagtgt gcggggataa tcaaaaacga
     3601 tacacaccgg ggtttaccgc gttaacggag cgcggcgttg tcccctcatg gtcgctggtc
     3661 cggtgcgatg gtggaagaaa ccggacgatg tgttacctcg caagctctgt tatgtcatgt
     3721 gtctgatttg tgatttaagt cggataattg tcgttgccat taagcagagg attgatgacc
     3781 gacagggtgg cattgttaga ataagactta ttcttatctg tgccgggaat gaaaatgaaa
     3841 agaaatcttc cgttaattat tttgttgtct tctctggtta tgggctgtac gcaacataaa
     3901 acagatatgc cccgacagtt ggttaaggca ttaccacaat atccggccta tgcagcggca
     3961 aattatataa agggacgggt tgatgtgaag tttgatattg gtgctgatgg tactgtcacc
     4021 cgaattgagt ttatccgttc agagccgcac catctgtttg atgagcaggt tgtaaaggcg
     4081 atggcaaaat ggcgatttga gaaggacagg ccgcgtaaag gcgtgaagaa aacgtttatc
     4141 tttagtcctt ctgcaccctg attatttcat cagaaattaa ttatcactct gttgttattc
     4201 tgtacatccc ggctgggtaa gtcttgttcc gccgggtatg aagatgaaat attgttggag
     4261 gacagtgggt acctgctcct gtaaccgaac gttcatttct cgttatttgt catgctggcc
     4321 ggacgcagat gcgttgcatc tgttgccagc cttctcctgc aggcttcaat aacccacgct
     4381 gaaaagttac cggacccttt atgctgaagg gcgatgttga tctgttcaat catgtgattg
     4441 gggaaacgga tattgcgggt tgtggttctg cgggtccggt ttttcgatga catatttatt
     4501 tcctttactg attgccatat gacggggatt ttacatggct gagcttcgta cactccagag
     4561 cagaatcaaa acactgaata cccgacgggt gaatattctg aagggtgaac agcgtcgtgt
     4621 cagtggcagt gcacgtgttt ccctcaagcg tcatatctgg ctcagggatg ccgggcagtg
     4681 ctgtctctgt ggtcgtgtgg ttgacctctg tgacagtgaa ctcgatcacc gaattgcact
     4741 tcagttcggt ggtggtaatg aggagacgaa tctctggacg ctctgtaccg aatgccatcg
     4801 acaaaagtct gctcgtgaag cggcgggtgg tatgccggac ccgacgctgc cggaggtgtc
     4861 cggaggtagt ggcagagcgg acgacattat cggactgtaa cccgaccggg gggggatcat
     4921 ccggcgtaaa aaacgatcgc tccggacacc gcgccccctc tcacgcagag aaaaaattcc
     4981 ccttcagaag ttataaacat gtttataacc tactgatgga tttttaccgt ttttttcttt
     5041 ttattcataa cgttgtggga ggtaaatgtt tatacctttt tttcgcaaaa tgtttacagg
     5101 gcagcggtaa acattttccc tgccgcctcc attcttaccg ggaggattgc tgacgttttt
     5161 ctgtttttcc ccggaggaaa gaatgctgac gacgcaaaaa agaaaatttg cgctggcgct
     5221 catgtcgggc aaaaacagaa cggctgctgc tattgaggct ggttactcgg aaaagtccgc
     5281 cagatgtaaa ggctcacaac tggcaaaaga tccggaagtg ctcgcgttta tagcccgtaa
     5341 gcagggcgag acggtggagg tggatgaggt tcctgtttac cggcagaaaa agacgcaggc
     5401 gtgccaccgg gaagttcctc cggtggaaaa gaaaaaccag gtttcagtgc caccggatga
     5461 cgggcaaagt ttctctccac tatccggtat tgattacatg gaggacggcc ttcctgatcc
     5521 tgtgaaagcc atgggtcgga tcctggtgga aaacatcagc attgatccga aactggctct
     5581 ggatgcagcc tggcgactgg cgcagttcac gcaccacaaa aaaggggatg ccggtaaaaa
     5641 atcggcaaaa ggtgacgcgg cgaaaaaagc ggctaaccgt tttgcggtgc caccaccacc
     5701 ccgcctggtg gtgaataatg ataatgaggg caacggatga tacctgtgtg gagcacggcc
     5761 tgcccggact gggcagagcg tctgaaaaag gggctgtcga ttattccggc tccgatttac
     5821 ccggaccagg ccgcacatgc cctggcgatt tttaaacaac tgcggattgt ggatgcaccg
     5881 ggcagcccga cgttcgggga gtcctgtgca ccgtgggtgt ttgatctggt ggctgccctg
     5941 tttggctcct acgatgcgca gaccggtgta cgccatataa aggaagtgtt cattctgata
     6001 cccaaaaaaa acagcaagtc cacgctggct gccgggatca tgatgacggc gctgttactg
     6061 aactggcggc aggcggcggg ctacaccatt ctggccccga ccgtggaggt ggcggctaac
     6121 gccttcaacc ctgccaggga tatggtacga cgggacgatg atctggatga cctctgtcag
     6181 gtgcagacac atatccggac catcacccat cgggtgacgg acaccaccct taaggtggtg
     6241 gctgccgatc cgaatacggt gtccggtatc aagtccgtgg ggacgctgat tgatgaactg
     6301 tggttatttg gcaagcagta caaagcggag gacatgttac gtgaagccat cggcggcctt
     6361 gcctcccgcc cggaagggtt tgtggtgtac acaaccaccc agtcgaatga gccgcccgcc
     6421 ggggtgttca gacagaaact gcagtacgcc cgggatgtgc gcgacggcaa aattaatgat
     6481 ccgcactttc tgccggtgat atttgagcat ccgccggaga tggtggagcg cggtgagcac
     6541 ctgctgatgg aaaacctcgc catggttaac ccgaatctcg gttattcggt ggatgaggct
     6601 tttctgtacc gggagtaccg taaagcccgg gaggctggtg aggaggcatt tcgtggcttc
     6661 atgtcaaaac acgccaatgt ggaaattggt cttgccctgc gttctgaccg ctgggcgggt
     6721 gcggatttct gggagcagca gggcaggcgc gtcagcctgg acgatatcct gcagcgcgct
     6781 gatgtggtga cggtggggat tgacggcggg ggcctggatg atctgctggg aatgtacgtg
     6841 attggtcgtg acagggaaac ccgcgaatgg ctgggctggg gccatgcctg ggcgcatgaa
     6901 accgcggtgg tcagacggaa gagtgaggca tcccggtttc aggattttgt ggccagtgga
     6961 gacatgacga ttgtccgtcg ggtcggggat gacacggcgg aagtggcgga gtatgtgcgt
     7021 cgtattcatg aggctgagtt actggaccat atcggtattg acccgtcagg tgtggggcag
     7081 attctggatt cactggcaga agccgggatc cccgacggaa ttgttgtggg gataagccag
     7141 ggctggaagc tgggcggggc catcaaaacc accgagcgca aactggctga gggagtgctg
     7201 gtgcatggtg gtcagccact gatggcctgg tgcgttggca atgcccgggt ggagcctaaa
     7261 ggtaacgcca tccttattac caaacaggcc agcggacggg ggaaaattga cccgctgatg
     7321 gcgctgttca atgcggtatc cctgatgtcc ctgaatccgg agcccaaaaa gaaagaatat
     7381 gcggtttttt tcatataacc ctgttcaccc tgtaaccatc atggaccgct gcggcggttt
     7441 ttttattttc aggaggctga tgtgactctt aaacgggcct gctccctgct gacggtgaaa
     7501 tccttcagtg aggatgagcg ggtgatcacc gggattgcgt caacgccttc tccggatcgg
     7561 gatggtgaca tcctggagcc ggagggcgcg gagtttggca gtgcgatccc gtttctctgg
     7621 cagcatgacc attcccgccc ggtggggcag tgtacggtgc gccgggtcag cgaagggctg
     7681 gaaatcacgg caacactggt gaagcccgta ccggatatgc cgtcgcaact ggctgcccgg
     7741 ctggatgagg tctgggcggc cattaagacc gggctggtca gggggctgtc cgtgggcttc
     7801 cgtccccatg aatacacctt tctggacgga ggtggactgc attttctgcg ctgggagctg
     7861 atggaggtgt ctgccgttac cgtgcccgcg aatgcggaat gcaccatccg gaccattaaa
     7921 tcttacgacc gcccgttttc tgccgcgtcc ggcaaccgga aaccggtggt gaaaatcgca
     7981 tcttctgccg gcgctgcggc acagtcaaca accgtttttc ataaggaaaa gaccataatg
     8041 aatattggcg aacagattaa aagttttgaa aacaagcgtg cagcgctggc agcctccctt
     8101 gaggaggtca tgaccaaagc cgcagaggaa gggcgcacgc tggatgtgga ggaggaagag
     8161 cattacgaca acaccgcagc ggaaatccgt caggtggatg cgcacctgaa gcgcctgcgt
     8221 gaactggaag ccggtaaggc cgccacggcg cagccggtga aacaggccgg taacgggaat
     8281 gtgaccgcgg tggcttctgc gccggtgatc cgtgtggagc agaaactgga taaggggatt
     8341 ggtttcgcac gttttgccaa atcgctggct gcggctaaag gtgtccgctc tgaagccctg
     8401 gaagtggccc gtcgtcagta tccggatgac agtcgtctgc atcatgtcct gaaatcggca
     8461 gtgggcgcgg ggaccaccac ggatccgcag tgggcaggca gcctgtctga atatcaggaa
     8521 tacgcacagg actttattga ttacctgcgt cctcagacca ttatcgggcg atttggtcag
     8581 ggcgggatcc ctgcacttcg tcaggtgccg ttcaatatcc gtgtgcacgc ccaggtgtcc
     8641 ggcggtgctg ccggctgggt gggtgagggt aaggcaaaac ccctgacgaa gtttgatttt
     8701 gaatccatca ccttcagtca tgcgaaggtg tcggccattg cggtactgac ggaagaattg
     8761 atccgttttt ccagtccggc tgctgatgca ctggtccgta atgcgctggc ggaagcggtg
     8821 gtggcgcgtc tggatacaga ctttgtggac ccgaaaaaag ccgcagtggc agatgtctcc
     8881 ccggcatcca tcacccatga tgtgaagggc acggcatcaa ccggtaaccc ggatgcggat
     8941 gcagaggcgg cgtttggaca gtttgtggca gcaaacctgc agcccaccgg tgcggtctgg
     9001 ctgatgtcca gcaccaatgc tctggcgctg tccatgcgta aaaatgcgct gggtcagaaa
     9061 gaatacccgg acatgaccct gctgggtggc tccttccagg ggctgccggt gattgtctcc
     9121 cagtacgtgg gtgaccagct ggtgctggtg aatgccccgg atatttatct ggcggatgac
     9181 ggcggcgtgg cagtggatat gtcccgcgag gcatcactgg aaatgcagtc tgagccgacc
     9241 ggcgacagta ccacgccgtc gccggtggag ctggtttcca tgttccagac aggcagtgtg
     9301 gccatccgtg cggagcgctg gatcaactgg cgtcgtcgcc gtaccgcggc ggtggcggtg
     9361 atcaccggtg tgaactacgg cagtgcgtcc ggcggctgag tctgataagg aggacgggag
     9421 gcgtgcgcct cccgtaacag gttatggcaa agatccgata cctgcagggc acgcatgatg
     9481 cccgggccgg ggatatccgg gatgtggcac agccgtgtgc ggaggtgctg gttcgcctgg
     9541 gaaaggcaga gtacatcacg gtgcgacgtc cggcaggtca gaaaaagaaa cgtgatgcgg
     9601 agcatggcga atgtggaacc ttttacggcg aacccgaaaa aaccagaaat caggacgtga
     9661 cgtaagagag gcgggctgga ccagcctgtt tcaggcggtg gctgagccct tttccggcgc
     9721 ctggcagcag ggcgtgaaag ccgatcctga agccgtcctc tcctttcatg cggtgtttgc
     9781 atgtatttcg ctgatatccc aggatatcgc caaaatgcgg ctgcgtctta tgcagacgga
     9841 tgcgcagggg atacgcaggg aaacgcgccg gggagatatt gcccgcctct gtcgtcgtcc
     9901 caacgcccag cagaaccgca tccagttttt tgaactgtgg ctgaacgcca aactgcgtca
     9961 cggcaatacg gtggtgctga aaatccgtaa tgcccggggg cagatcaaag aactgcgtat
    10021 tctggactgg aaccgggttg aacctctggt ggcggatgac ggcgaggtgt tctaccgcat
    10081 cacgccggac cggaactgtg ggatcactga ggcggtgacg gtgcctgccc gggaagtgat
    10141 ccacgatcgg tttaactgtt tttttcatcc gcttataggg ttgccgccgg tgtatgccgc
    10201 cgggctggcg gccacgcagg ggcatcatat tcaggaaaat tcgacgtctt ttttcagaaa
    10261 tggcggcagg ccgtccgggg tgattgagat ccccggcagt attacggaag aaaatgcgaa
    10321 aaaactgaag agcaactggg acagcgggta tacaggcgaa aatgcgggga aaacggccat
    10381 tctgagtaac ggggcaaaat acaaccccac gacgttttca ccggtggatg cgcagacggt
    10441 ggaacaactg aagatgaccg ctgaaattgt ctgttcggtg ttccgtgtcc cggcctacaa
    10501 gattggcgtg ggacaaccgc cttccagtga caacgtggag gcgctggagc agcagtatta
    10561 ttcccagtgc ctgcagacac tgattgagtc cattgagctg ttactggatg aggcgctgga
    10621 aacgggggaa aacgagagta cggaatttga tgtcaccacg ctgctgagaa tggacagtga
    10681 gcggcgcatg aaaacgctgg gggatgcggt gaaaaatacg cttctcacgc ccaatgaagc
    10741 ccgtaaacgg gagaacctgc cgcccctggc aggaggcgat gcactgtatc ttcagcagca
    10801 gaactacagt ctggaagcgt tgtcccgccg tgatgcccgt gaagatccgt tcgcgtcgtc
    10861 cggtaaaaca gcttccgtgc ctcaggcggt cgctgcatct gacggtaata aggcaatcac
    10921 tgaaacagag catgacgcgg tgaaggcgat gttcaggggg attctgaaaa aatgaatgaa
    10981 cgtgaactgt ccattatccg tgcgctgggt gaagaatttt ccgcggtgct ggcggattta
    11041 cagcgcacat ttgaggagaa aatagccgcg caggcacaaa cgtttgaaga aaaactggct
    11101 tccctgtctg tggtattaca gaagtgcgtg acgggcgatg atgtgcgtcc gatgcttgag
    11161 cagatggtga aggaggcggt aagccatatc cctgttccgc gagatggtcg tgactacgat
    11221 ccggaagt
//
LOCUS       DAZFLD010000154        11121 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.155, whole genome shotgun sequence.
ACCESSION   DAZFLD010000154 DAZFLD010000000
VERSION     DAZFLD010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11121)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11121)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11121
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.155"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..390)
                     /locus_tag="ACHKBL_003546"
     CDS             complement(<1..390)
                     /locus_tag="ACHKBL_003546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFO5820363.1"
                     /translation="MTIQQWLFSFKGRIGRRDFWIWIGLWFAGMLVLFSLAGKNLLDI
                     QTAAFCLVCLLWPTAAVTVKRLHDRGRSGAWAFLMIVAWMLLAGNWAILPGVWQWAVG
                     RFVPTLILVMMLIDLGAFVGTQGENKYG"
     gene            491..1090
                     /gene="yiiQ"
                     /locus_tag="ACHKBL_003547"
     CDS             491..1090
                     /gene="yiiQ"
                     /locus_tag="ACHKBL_003547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiQ family protein"
                     /protein_id="HFO5820364.1"
                     /translation="MKPGCTLFFLLCSALTVTTTAHAQTPDTATTAPYLLAGAPTFDL
                     SISQFREDFNSQNPSLPLNEFRAIDSSPDKANLTRAASKINENLYASTALERGTLKIK
                     SIQMTWLPIQGPEQKAAKAKAQAYMAAVIRTLTPLMTKTQSQKKLQALLTAGKNKRYY
                     TETEGALRYVVADNGEKGLTFAVEPIKLALSESLEGLNK"
     gene            1198..1965
                     /gene="tpiA"
                     /locus_tag="ACHKBL_003548"
     CDS             1198..1965
                     /gene="tpiA"
                     /locus_tag="ACHKBL_003548"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HFO5820365.1"
                     /translation="MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPE
                     MYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYH
                     KESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFE
                     GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNA
                     AELFAQPDIDGALVGGASLKADAFAVIVKAAEAAKQA"
     gene            complement(2020..2775)
                     /gene="cdh"
                     /locus_tag="ACHKBL_003549"
     CDS             complement(2020..2775)
                     /gene="cdh"
                     /locus_tag="ACHKBL_003549"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008715 - CDP-diacylglycerol diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HFO5820366.1"
                     /translation="MKKAGLLFLVMIVIAVVAAGIGYWKLTGEESDTLRKIVLEECLP
                     NQQQNQNPSPCAEVKPNAGYVVLKDLNGPLQYLLMPTYRINGTESPLLTDPSTPNFFW
                     LAWQARDFMSKKYGQPVPDRAVSLAINSRTGRTQNHFHIHISCIRPDVREQLDNNLAN
                     ISSRWLPLPGGLRGHEYLARRVTESELVQRSPFMMLAEEVPEAREHMGSYGLAMVRQS
                     DNSFVLLATQRNLLTLNRASAEEIQDHQCEILR"
     gene            complement(2881..3870)
                     /gene="sbp"
                     /locus_tag="ACHKBL_003550"
     CDS             complement(2881..3870)
                     /gene="sbp"
                     /locus_tag="ACHKBL_003550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter
                     substrate-binding protein Sbp"
                     /protein_id="HFO5820367.1"
                     /translation="MNKWGVGLTFLLAATSVMAKDIQLLNVSYDPTRELYEQYNKVFS
                     AHWKQQTGDNVVIRQSHGGSGKQATSVINGIEADVVTLALAYDVDAIAERGRIDKEWI
                     KRLPDNSAPYTSTIVFLVRKGNPKQIHDWNDLIKPGVSVITPNPKSSGGARWNYLAAW
                     GYALHHNNNDQAKAQDFVRALYKNVEVLDSGARGSTNTFVERGIGDVLIAWENEALLA
                     ANELGKDKFEIVTPSESILAEPTVSVVDKVVEKKGTKEVAEAYLKYLYSPEGQEIAAK
                     NYYRPRDAEVAKKYENAFPKLKLFTIDEEFGGWTKAQKEHFANGGTFDQISKR"
     gene            complement(4190..5152)
                     /gene="pfkA"
                     /locus_tag="ACHKBL_003551"
     CDS             complement(4190..5152)
                     /gene="pfkA"
                     /locus_tag="ACHKBL_003551"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014072359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HFO5820368.1"
                     /translation="MIKKIGVLTSGGDAPGMNAAIRGVVRSALTEGLEVMGIYDGYLG
                     LYEDRMVQLDRYSVSDMINRGGTFLGSARFPEFRDENIRAVAIENLKKRGIDALVVIG
                     GDGSYMGAMRLTEMGFPCIGLPGTIDNDIKGTDYTIGFFTALSTVVEAIDRLRDTSSS
                     HQRISVVEVMGRYCGDLTLAAAIAGGCEFVVVPEVEFSREDLVNEIKAGIAKGKKHAI
                     VAITEHMCDVDELAHFIEKETGRETRATVLGHIQRGGSPVPYDRILASRMGAYAIELL
                     LAGYGGRCVGIQNEQLVHHDIIDAIENMKRPFKGDWLDCAKKLY"
     gene            complement(5333..6235)
                     /gene="fieF"
                     /locus_tag="ACHKBL_003552"
     CDS             complement(5333..6235)
                     /gene="fieF"
                     /locus_tag="ACHKBL_003552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312867.1"
                     /note="FieF, a metal efflux transporter, is a member of
                     the CDF (cation diffusion facilitator) family of
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family cation-efflux transporter FieF"
                     /protein_id="HFO5820369.1"
                     /translation="MNQSYGRLVSRAAIAATAMASLLLLIKIFAWWYTGSVSILAALV
                     DSLVDIGASLTNLLVVRYSLQPADDNHSFGHGKAESLAALAQSMFISGSALFLFLTGI
                     QHLISPTPMTDPGVGVIVTIVALICTIILVSFQRWVVRRTQSQAVRADMLHYQSDVMM
                     NGAILLALGLSWYGWHRADALFALGIGIYILYSALRMGYEAVQSLLDRALPDEERQEI
                     IDIVTSWPGVSGAHDLRTRQSGPTRFIQIHLEMEDSLPLVQAHMVADQVEQAILRRFP
                     GSDVIIHQDPCSVVPREGKRSMLS"
     gene            complement(6384..6884)
                     /gene="cpxP"
                     /locus_tag="ACHKBL_003553"
     CDS             complement(6384..6884)
                     /gene="cpxP"
                     /locus_tag="ACHKBL_003553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-envelope stress modulator CpxP"
                     /protein_id="HFO5820370.1"
                     /translation="MRIVTAAVMASTLAVSSLSHAAEVGSGDNWHPGEELTQRSTQSH
                     MFDGISLTEHQRQQMRDLMQQARHEQPPVNVSELETMHRLVTAENFDENAVRAQAEKM
                     ANEQIARQVEMAKVRNQMYRLLTPEQQAVLNEKHQQRMEQLRDVTQWQKSSSLKLLSS
                     SNSRSQ"
     gene            7034..7732
                     /gene="cpxR"
                     /locus_tag="ACHKBL_003554"
     CDS             7034..7732
                     /gene="cpxR"
                     /locus_tag="ACHKBL_003554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response regulator transcription
                     factor CpxR"
                     /protein_id="HFO5820371.1"
                     /translation="MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDDS
                     IDLLLLDVMMPKKNGIDTLKALRQTHQTPVIMLTARGSELDRVLGLELGADDYLPKPF
                     NDRELVARIRAILRRSHWSEQQQNNDNGSPTLEVDALSLNPGRQEATFDGQTLELTGT
                     EFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPDRKDGHPWF
                     KTLRGRGYLMVSAS"
     gene            7729..9102
                     /gene="cpxA"
                     /locus_tag="ACHKBL_003555"
     CDS             7729..9102
                     /gene="cpxA"
                     /locus_tag="ACHKBL_003555"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress sensor histidine kinase CpxA"
                     /protein_id="HFO5820372.1"
                     /translation="MIGSLTARIFAIFWLTLALVLMLVLMLPKLDSRQMTELLDSEQR
                     QGLMIEQHVEAELANDPPNDLMWWRRLFRAIDKWAPPGQRLLLVTTEGRVIGAERSEM
                     QIIRNFIGQADNADHPQKKKYGRVELVGPFSVRDGEDNYQLYLIRPASSSQSDFINLL
                     FDRPLLLLIVTMLVSTPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEF
                     LAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRSGESKELER
                     IETEAQRLDSMINDLLVMSRNQQKNALVSETIKANQLWSEVLDNAAFEAEQMGKSLTV
                     NFPPGPWPLYGNPNALESALENIVRNALRYSHTKIEVGFAVDKDGITITVDDDGPGVS
                     PEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIW
                     LPLYKRS"
     gene            complement(9208..9882)
                     /gene="yiiM"
                     /locus_tag="ACHKBL_003556"
     CDS             complement(9208..9882)
                     /gene="yiiM"
                     /locus_tag="ACHKBL_003556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-hydroxyaminopurine reductase"
                     /protein_id="HFO5820373.1"
                     /translation="MRYPVDVYTGKIQAYPEGKPSAIAKIQVDGELMLTDLGLEGDEQ
                     AEKKVHGGPDRALCHYPREHYLYWAREFPEQAELFVAPAFGENLSTDGLTESNVYIGD
                     IFRWGEALIQVSQPRSPCYKLNYHFDISDIAQLMQNTGKVGWLYSVIAPGLVSADAPL
                     ELVSRVSDVTVQEAAAIAWHMPFDDDQYHRLLSAAGVSKSWTRTMQKRRLSGKIEDFS
                     RRLWGK"
     gene            complement(10031..11014)
                     /gene="kdgT"
                     /locus_tag="ACHKBL_003557"
     CDS             complement(10031..11014)
                     /gene="kdgT"
                     /locus_tag="ACHKBL_003557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015649 - 2-keto-3-deoxygluconate:proton
                     symporter activity [Evidence IEA];
                     GO_process: GO:0046411 - 2-keto-3-deoxygluconate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate transporter"
                     /protein_id="HFO5820374.1"
                     /translation="MQIKRSIEKIPGGMMLVPLFLGALCHTFSPEAGKYFGSFTNGMI
                     TGTVPILAVWFFCMGASIKLSATGTVLRKSGTLVVTKIAVAWVVAAIASRIIPEHGVE
                     VGFFAGLSTLALVAAMDMTNGGLYASIMQQYGTKEEAGAFVLMSLESGPLMTMIILGT
                     AGIASFEPHVFVGAVLPFLVGFALGNLDPELREFFSKAVQTLIPFFAFALGNTIDLTV
                     IAQTGLLGILLGVAVIIVTGIPLIIADKLIGGGDGTAGIAASSSAGAAVATPVLIAEM
                     VPAFKPMAPAATSLVATAVIVTSILVPILTSIWSRKVKARAAKIEILGTVK"
BASE COUNT         2834 a         2968 c         2696 g         2623 t
ORIGIN      
        1 accatattta ttttcgcctt gtgtgccaac aaacgcacca agatcgataa gcatcatcac
       61 cagaatcaat gtcgggacaa aacgccccac cgcccattgc cagacacccg gtaaaattgc
      121 ccagttaccc gccagcagca tccacgccac aatcatcaga aatgcccatg cgccggaacg
      181 cccgcgatca tgcaagcgct taacggttac tgccgctgtt ggccaaagca agcacacaag
      241 gcaaaacgcc gcggtctgaa tatcgagtaa atttttaccc gccagtgaga aaagcaccag
      301 catgcctgcg aaccacaggc ctatccaaat ccagaaatca cggcgtccaa tacgcccttt
      361 aaatgagaat aaccattgct gtatggtcat gtaagttcct tgatggttgt cttttccagg
      421 attctaccct tttgacaagg gcgacagata tcgttttaat cggagccagt cataacaaaa
      481 ggtactgtca atgaagccag ggtgtacgct gttttttctc ttatgttctg cattaaccgt
      541 tacaacaacg gcgcatgcac aaacaccaga tacggcaacg accgcgcctt atctgctggc
      601 tggagcccct actttcgatc tctccatcag ccagtttcga gaagacttta acagccagaa
      661 tcccagcctg ccactgaacg aatttcgtgc catcgacagc agtcccgaca aagccaatct
      721 cactcgtgca gcaagtaaaa ttaacgagaa cttgtatgct tctactgcgc tggagcgcgg
      781 taccttaaaa atcaaaagca ttcaaatgac ctggctaccc atccaggggc cagagcaaaa
      841 agccgcgaaa gcgaaagctc aggcatacat ggcagcggtg atccgcacac tcaccccatt
      901 aatgaccaaa actcaaagcc agaaaaaact gcaggcgcta ctaacggcgg ggaaaaacaa
      961 acgttattac accgagacag aaggtgcact gcgttatgtt gtcgcggaca acggcgaaaa
     1021 ggggctgacc ttcgctgttg aaccgattaa gctggcgcta tctgaatcgc ttgaaggttt
     1081 gaataaatga caaaaagcaa agcctttgtg ccgatgaatc tctatactgt ttcacagacc
     1141 tgctgccctg cggggcggcc atcttccttt attcgcttat aagcgtggag aattaaaatg
     1201 cgacatcctt tagtgatggg taactggaaa ctgaacggca gccgccacat ggttcacgag
     1261 ctggtatcta acctgcgtaa agagctggca ggtgttgctg gctgtgcggt tgcaatcgca
     1321 ccaccggaaa tgtacatcga tatggcgaag cgtgaagctg aaggcagcca catcatgctg
     1381 ggtgcgcaaa acgtggacct gaacctgtcc ggcgcattca ccggtgaaac ctctgctgct
     1441 atgctgaaag acatcggcgc acagtacatc atcatcggcc actctgaacg tcgtacttac
     1501 cacaaagagt ctgacgaact gatcgcgaaa aaattcgcgg tgctgaaaga gcagggtctg
     1561 actccggttc tgtgcatcgg tgaaaccgaa gctgaaaacg aagcgggcaa aactgaagaa
     1621 gtttgtgcac gtcagatcga cgcggtactg aaaactcagg gtgctgcggc attcgaaggt
     1681 gcggttatcg cttacgaacc cgtatgggca atcggtactg gcaaatctgc aactccggct
     1741 caggcacagg ctgttcacaa attcatccgt gaccacatcg ctaaagttga cgctaacatc
     1801 gctgaacaag tgatcattca gtacggcggc tctgtaaacg cgtctaacgc tgcagaactg
     1861 tttgctcaac cagacatcga cggcgcgctg gttggcggtg cttctctgaa agctgacgct
     1921 ttcgcagtga tcgttaaagc tgcagaagcg gctaaacagg cttaagtctg acaggtgccg
     1981 gatttcatat ccggcactta ctttccttaa ctcctcgcct taacgcaaaa tctcacactg
     2041 atgatcctgg atttcctcgg ctgaagcacg gttaagcgtc agtagatttc gttgtgtcgc
     2101 cagcaataca aatgagttat cactctgccg caccatcgcc agcccgtagc ttcccatatg
     2161 ttcccgcgcc tcaggcactt cttctgcaag catcataaat gggctgcgtt gtaccagttc
     2221 gctttccgtt acccgacgcg ccaggtattc atgcccgcgc aaaccacctg gcagtggcaa
     2281 ccagcggctg ctgatgttcg ccagattgtt atccagctgt tcgcgcacat caggacgaat
     2341 acaagagata tgaatatgaa aatggttttg cgtacgcccg gtgcgagagt tgatcgccaa
     2401 agaaaccgcg cgatcgggaa ccggctggcc gtattttttg ctcataaaat cacgcgcctg
     2461 ccaggccaac caaaagaagt tcggcgttga aggatcggtc aacaaaggac tttcagtacc
     2521 gttaatacga tacgttggca tcaacagata ttgcagtggg ccattaagat cttttaaaac
     2581 cacgtatccg gcattgggtt tgacttccgc acatggcgaa ggattttgat tttgctgctg
     2641 attgggcaaa cattcctcaa ggacaatctt acgtaatgta tccgactctt caccggttaa
     2701 tttccagtaa ccaataccgg cagccacaac ggcgataact atcatcacca aaaaaagaag
     2761 acccgctttt ttcatctttt ttccctgtac ctcaaagaga tgcaagggta acgcaaaatc
     2821 gtgacaaata aaaaacccag tcgcgagaaa ccaccgggtt tgataattat cctggagaaa
     2881 tcagcgtttg ctaatctgat cgaacgtacc gccgttagca aaatgctctt tttgcgcttt
     2941 cgtccagccg ccgaactctt catcaatggt gaataacttc agctttggaa acgcattttc
     3001 gtactttttc gccacctcag catcgcgcgg acggtagtag tttttcgcgg caatttcctg
     3061 accttctggc gagtagagat atttcaggta ggcttccgcc acctctttgg tgcctttttt
     3121 ctcgaccact ttatcgacca ccgacacggt tggctctgcg aggatagact cactcggcgt
     3181 gacaatttcg aatttatctt tccccagttc attcgctgcc agcagagctt cgttttccca
     3241 ggcaatcagt acatcgccaa tcccgcgctc gacaaaggta ttagtggagc cgcgcgcgcc
     3301 agaatccaga acttcgacgt ttttatacag tgcccgaaca aaatcctgtg cttttgcctg
     3361 atcgttgttg ttgtgatgca gcgcgtagcc ccaggctgcc aggtagttcc agcgcgcgcc
     3421 accagagctt ttcggattag gcgtgatcac cgaaacaccc ggtttaatca gatcgttcca
     3481 gtcatggatc tgcttcggat tgcccttacg caccaggaaa acaatggtgg aagtgtacgg
     3541 tgcggagtta tccggcagac gtttgatcca ctctttatca atccgcccgc gttccgcaat
     3601 cgcgtccacg tcataggcca gagccagcgt gacaacatca gcttcaatac cgttgattac
     3661 cgacgtcgct tgtttgcctg aaccaccgtg ggactgacgg atcaccacgt tatcgccagt
     3721 ttgctgtttc cagtgggcgc tgaatacctt gttgtactgt tcgtacaatt cgcgcgttgg
     3781 atcatatgaa acgttaagaa gctgaatatc ctttgccata acgctggttg ccgccagcaa
     3841 aaatgttaac cctacgcccc acttgttcat cgcccgactc tcttatgttg tgttgtgatg
     3901 agcaaagcgt gccagaaggt taaccaaaca ttaaagaata aaaaaagatt ggctataact
     3961 tgcgggtata tgtaggggga ttaaaaaggc ggaagaaatc cgcctcatat tgctgacaaa
     4021 gtgcgctttg tccatgccgg atgcggcgcg aacgccttat ccggcctaca aaagttcgca
     4081 aattcaataa attgcagaat tcatgtaggc ctgataagcg aagcgcatca ggcatttttg
     4141 cttctgtcat cggtttcagg ctaaaggaat ctgccttttt ccgaaatcat taatacagtt
     4201 ttttcgcgca gtccagccag tcgcctttga acggacgctt catgttttcg atagcgtcga
     4261 tgatgtcgtg gtgaaccagc tgttcgttct ggatacctac gcaacgacca ccgtaacctg
     4321 ccagcagcag ctcgatagcg taagcgccca tacgggaagc cagaatacgg tcgtaaggca
     4381 ccggagaacc accgcgctgg atgtggccca gcacagttgc gcgggtttca cgaccggttt
     4441 ctttctcgat gaaatgcgcc agttcgtcaa catcacacat atgttcggta atcgccacta
     4501 tcgcgtgttt tttacctttc gcgatacccg ctttgatttc gtttaccagg tcttcacggc
     4561 tgaattcaac ttccggaacc acaacgaatt cacagccacc ggcaatagcc gcagccaaag
     4621 tcagatcgcc acaataacgg cccatcactt ccaccacgga aatacgctgg tgagaagaag
     4681 aggtgtcacg cagacggtcg atcgcttcta caacggtgct cagcgcagtg aagaaaccga
     4741 tagtgtagtc agtgcctttg atgtcgttgt cgatagtgcc cggcagaccg atgcacggga
     4801 agcccatttc ggtcagacgc attgcaccca tgtaggaacc gtcaccgccg ataaccacca
     4861 gcgcgtcgat accacgtttt ttcaggtttt cgatagccac ggcgcggatg ttctcgtcgc
     4921 ggaattccgg gaaacgcgca gaaccgagga acgtaccacc acggttgatc atgtcagaaa
     4981 cgctgtaacg gtctagctgt accatacggt cttcatacag acccagatag ccgtcataaa
     5041 tgcccattac ttccagacct tctgtcagcg cagaacgaac aaccccgcga attgcggcgt
     5101 tcatgcctgg cgcatcaccg ccgcttgtca acacaccgat tttcttaatc atgactacct
     5161 ctgaactttg gaatgcaaaa tgaaatctgt tgccggaagt cttcttgcac atcgaagtga
     5221 tccaacgaat gtgcaaatag tataacaatc acttcctgct gaattgattc aggtcaggcc
     5281 aaatggcggt attttataca caaaatgcgg gtctggctct cttttatact gattatgaaa
     5341 gcatagaccg tttaccctcc ctgggtacga cggaacaggg gtcctgatgg ataatcacat
     5401 ccgatcccgg aaaacgccgt aaaatagcct gctctacctg atccgccacc atatgtgcct
     5461 gaaccaaagg cagagagtct tccatttcca aatgaatctg aataaagcgg gtcggccctg
     5521 actgccgcgt gcgaagatcg tgagcgccgc taacacccgg ccaggaagtc acgatatcaa
     5581 taatttcttg ccgttcttca tcaggcaatg cgcgatccag taatgactgt accgcctcat
     5641 atcccatgcg taacgcgcta tataaaatat agatgccgat tcccaatgca aacagagcat
     5701 cggcgcgatg ccagccgtac caggacaacc ccagcgccag cagaattgca ccgttcatca
     5761 taacatcaga ctggtaatgt agcatatcag cccgcaccgc ctggctttgc gtccggcgca
     5821 ccacccaacg ctgaaacgag acaaggataa tcgtacaaat tagcgccaca attgtcacga
     5881 taaccccgac gcctggatct gtcatcggtg ttggagatat cagatgttga atacccgtca
     5941 aaaacaggaa tagtgctgaa ccggagataa acatactttg cgccagcgcc gcgagggact
     6001 cagctttacc gtgaccaaac gagtgattat cgtcggcagg ttgcagggaa tatcgcacca
     6061 ccagtaaatt cgtcaacgac gcgccgatat ccaccagcga atccaccagc gcggcgagaa
     6121 tactcaccga cccggtatac caccatgcaa aaattttaat cagcaatagc agtgaagcca
     6181 tcgccgttgc agcaatcgcc gcccgactga ccagccgtcc ataagattga ttcataaata
     6241 ctcccgctat caactgacgc tagtataacg gaagcaaatc atctgcaatg cattaagcag
     6301 caggcaaatt gaggataaaa aaaaccccca catcatgtgg gggaagacag ggatggtgtc
     6361 tatggcaagg aaaacagggt ttactactgg gaacgtgagt tgctactact caatagcttc
     6421 aacgatgaac ttttttgcca ttgcgtcacg tcacgcaact gctccattcg ttgttgatgt
     6481 ttctcattta aaaccgcttg ctgctccggc gttaacaggc gatacatttg gttgcggact
     6541 tttgccatct caacctgacg agcaatttgc tcattcgcca ttttttctgc ctgtgcgcgc
     6601 acagcgtttt catcaaaatt ttctgcggtg acaaggcgat gcattgtctc cagttcgcta
     6661 acattaacag gaggctgttc gtgccgggcc tgttgcataa gatctcgcat ctgctgacgc
     6721 tgatgttcgg ttaaacttat gccgtcgaac atatggctct gcgtactgcg ctgcgtaagt
     6781 tcttcacccg gatgccagtt atcgcctgaa ccgacttcag cagcgtggct taatgaactg
     6841 actgccagcg ttgaggccat gacggcagcg gtaactatgc gcatcatttg ctcccaaaat
     6901 ctttctgtcg cgattcaacg atagagagtt tacgattcag gctgcaaaca tgcgtcaggg
     6961 ggtgtaaaac aacgtaaagt catggattag cgacgtctga tgacgtaatt tctgcctcgg
     7021 aggtatttaa acaatgaata aaatcctgtt agttgatgat gaccgagagc tgacttccct
     7081 attaaaggag ctgctcgaga tggaaggctt caacgtgatt gttgcccacg atggggaaca
     7141 ggcgcttgat cttctggacg acagcattga tttacttttg cttgatgtaa tgatgccgaa
     7201 gaaaaatggt atcgacacat taaaagcact tcgccagaca caccagacgc ctgtcattat
     7261 gttgacggcg cgcggcagtg aacttgatcg cgttctcggc cttgagctgg gcgcagatga
     7321 ctatctcccg aaaccgttta atgatcgtga gctggtggca cgtattcgcg cgatcctgcg
     7381 ccgttcgcac tggagcgaac aacagcaaaa caacgacaac ggttcaccga cactggaagt
     7441 tgatgcctta agtctcaatc ctggtcgcca ggaagccacc ttcgacgggc aaacactgga
     7501 gttaaccggt actgagttta ccctgctcta tttgctggca cagcatctgg gtcaggtggt
     7561 ttcccgtgag catttaagcc aggaagtgct gggcaagcgc ctgacgcctt tcgaccgcgc
     7621 tatcgatatg catatttcca acctgcgtcg taaactgccg gatcgtaagg atggtcaccc
     7681 gtggtttaaa accctgcgtg gtcgtggcta tctgatggtt tctgcttcat gataggcagc
     7741 ttaaccgcgc gcatcttcgc catcttctgg ctgacgctgg cgctggtgtt gatgttggtt
     7801 ttgatgttac ccaagctcga ttcacgccag atgaccgagc ttctggatag cgaacagcgt
     7861 cagggtctga tgattgagca gcatgttgaa gcggagctgg cgaacgatcc gcccaacgat
     7921 ttaatgtggt ggcggcgtct gttccgggcg attgataagt gggcaccgcc aggacagcgt
     7981 ttgttattgg tgaccaccga aggccgcgtg atcggcgctg aacgcagcga aatgcagatc
     8041 attcgtaact ttattggtca ggccgataac gccgatcatc cgcagaagaa aaagtatggc
     8101 cgcgtggaac tggtcggtcc gttctccgtg cgtgatggcg aagataatta ccaactttat
     8161 ctgattcgtc cggccagcag ttctcaatcc gatttcataa acttactgtt tgaccgcccg
     8221 ctattactgc tgattgtcac catgctggtc agtacgccgc tgctgttgtg gttggcctgg
     8281 agtctggcaa aaccggcgcg taagctgaaa aacgctgccg atgaagttgc ccaaggaaac
     8341 ttacgccagc atccggaact ggaagcgggg ccacaggaat tccttgccgc cggtgccagt
     8401 tttaaccaga tggtcaccgc gctggagcgc atgatgacct ctcagcaacg tctactttct
     8461 gatatctctc acgaactgcg caccccgctg acgcgtctgc aactgggtac ggcgttactg
     8521 cgccgtcgta gcggtgaaag caaggaactg gagcgtattg aaaccgaagc gcaacgtctg
     8581 gacagcatga tcaacgatct gttggtgatg tcacgtaatc agcagaaaaa tgcgctggtt
     8641 agcgagacca tcaaagccaa ccagttgtgg agtgaagtgc tggataacgc ggcgttcgaa
     8701 gccgagcaga tgggcaagtc gttgacggtt aacttcccgc ctgggccgtg gccgctgtac
     8761 ggcaacccga acgccctgga gagtgcgctg gaaaacattg ttcgtaatgc cctgcgttat
     8821 tcccatacga agattgaagt gggctttgcg gtagataaag acggtatcac cattacggtg
     8881 gacgacgatg gtcctggcgt tagcccggaa gatcgcgaac agattttccg tccgttctat
     8941 cgtaccgatg aagcacgcga tcgtgaatct ggcggtacag gtttggggct ggcgattgta
     9001 gaaaccgcca ttcagcagca tcgtggctgg gtgaaggcag aagacagccc gctgggcggt
     9061 ttacggctgg tgatttggtt gccgctgtat aagcggagtt aaactccgca tttgtaggca
     9121 ggataaggcg tttacgccgc atccggcatt tgagcaggat gcctgatgcg acgctgatag
     9181 cgtcttatca ggcctacact ccggggatta ttttccccac aatcggcggg aaaaatcttc
     9241 gatcttgccg ctcagtcggc gcttttgcat cgttcgcgtc cagcttttcg atacccctgc
     9301 ggcggagagt aaacggtgat actgatcgtc atcaaacggc atatgccatg cgatggcggc
     9361 agcctcctgc acggtcacat cgctgacacg ggaaaccaac tccagtggcg catcagcaga
     9421 taccaaccct ggtgcaatta cgctatacaa ccaccccact ttacccgtgt tttgcatcaa
     9481 ctgcgctata tcgctgatat caaaatggta attgagttta tagcaaggtg agcgtggctg
     9541 gctgacctga attaatgcct ctccccagcg gaaaatatcg ccaatataaa cattactttc
     9601 cgtcagaccg tcggttgaga gattttcgcc aaacgcaggc gcaacaaaca actccgcctg
     9661 ttccggaaat tcccgcgccc agtaaagata atgctcacga ggataatgac acagcgctct
     9721 gtctggcccg ccgtgaacct ttttctccgc ctgctcgtca ccttccagcc ccaggtccgt
     9781 cagcatcaac tcaccatcaa cctggatttt ggcaattgcg ctgggtttgc cttcgggata
     9841 agcctgaatc ttgcctgtgt atacatcaac cggatatcgc atctgttccc tctccacgag
     9901 aaatttcccc attagaccat atttatttta tgtcaggcgt gctgttttcg cggtaagtat
     9961 ccaggcagga actcaatagc aaaccggacg aacgttagtt catccggctg tggggattaa
    10021 ggaagcaggt ttatttcacc gtacctaaaa tttcgatttt cgctgctctg gctttgactt
    10081 tacgtgacca gatagaggta agaattggca ccagaatcga agtcacaatg accgccgtcg
    10141 cgaccagcga agttgctgcc ggagccatcg gtttaaacgc aggcaccatt tctgcaatca
    10201 gcacaggtgt cgctaccgcg gcccctgcgg aactggaagc ggcaattccg gcagtgccat
    10261 cgccaccgcc aatcaattta tcagcgataa tcaacggaat accggtcacg ataattactg
    10321 ccacacccaa caggatcccc agcaaacctg tctgggcaat taccgtcaaa tcaatggtat
    10381 tacccagtgc gaaggcaaag aatggaatca gcgtctgcac cgctttgctg aaaaattctc
    10441 gtaattcagg gtcaaggttc ccaagggcaa agcccaccag gaacggtaat actgcgccga
    10501 cgaaaacatg cggttcaaac gaggcaatcc cggcagtgcc cagaataatc atcgtcatga
    10561 gcggcccgga ctccaatgac atcaacacaa atgccccagc ttcttctttt gtgccgtact
    10621 gctgcatgat ggaagcgtaa agtccgccgt tggtcatatc catcgccgcc accagcgcca
    10681 gcgttgaaag tccggcaaag aatccaactt caacaccatg ttccggaata atgcgcgagg
    10741 caattgccgc aaccacccac gcgacggcaa tttttgttac caccagcgta ccggatttac
    10801 gcaataccgt tcccgtcgcg cttaatttta ttgacgcccc catgcaaaaa aaccacaccg
    10861 ccagaatggg caccgtaccg gtaatcatcc cgttggtgaa tgatccaaaa tatttccccg
    10921 cctccggcga gaaggtgtgg cacagtgcgc caaggaataa cgggacgagc atcatccccc
    10981 ccgggatttt ctcaatcgag cgttttatct gcatttccat cacctgtcgg catattttta
    11041 agggctgatg cgtcattcct caatggagca tcagtgttga tggaaacgaa gatagacatt
    11101 catttaatca ataaaagtga t
//
LOCUS       DAZFLD010000155        11081 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.156, whole genome shotgun sequence.
ACCESSION   DAZFLD010000155 DAZFLD010000000
VERSION     DAZFLD010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11081)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11081)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11081
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.156"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            205..1419
                     /gene="manD"
                     /locus_tag="ACHKBL_003558"
     CDS             205..1419
                     /gene="manD"
                     /locus_tag="ACHKBL_003558"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0016052 - carbohydrate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-mannonate dehydratase ManD"
                     /protein_id="HFO5820375.1"
                     /translation="MKIIAADVFVTCPGRNFVTLKITTASGLCGLGDATLNGRELSVA
                     SYLKDHLCPQLIGRDASRIEDIWQFFYKGAYWRRGPVTMSAISAIDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGRTIDEVLEDYAKHQQMGFKAIRVQCGVPGMQTTYG
                     LAKGKGLAYEPATKGLWPEEQLWSSEKYLDFTPKLFEAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRLFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIDYIRTTITHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCHAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPHSWRFDEGYMHPGDEPGLGISFDEKLAAKYPYDPAYLP
                     VARLEDGTLWNW"
     gene            1486..2769
                     /locus_tag="ACHKBL_003559"
     CDS             1486..2769
                     /locus_tag="ACHKBL_003559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000382182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO5820376.1"
                     /translation="MDVDVSTSVAGNKPQRIRRIQTVTLVLLFMAGIVNFLDRSSLSV
                     AGEAIRGELGLSATEFGVLLSAFSLSYGFSQLPSGILLDRFGPRIVLGAGLIFWSLMQ
                     ALTGMVNSFSHFILMRIGLGIGEAPFMPAGVKSITDWYAQKERGTALGIFNSSTVIGQ
                     AIAPPALVLMQLAWGWRTMFVIIGVAGILVGICWYAWYRNRAQFVLTEEERTYLSAPV
                     KPRPQLQFSEWLALFKHRTTWGMILGFSGVNYTGWLYIAWLPGYLQAEQGFSLAKTGW
                     VAAIPFLAAAVGMWVNGIVVDRLAKKGYDLAKTRKTAIVCGLMMSALGTLLVVQSSSP
                     AQAVAFISMALFCVHFAGTSAWGLVQVMVSETKVASIAGIQNFGSFVFASFAPIVTGW
                     VVDTTHSFNLALVIAACVTFTGALCYFFIVKDRIE"
     gene            3021..3938
                     /gene="oxyR"
                     /locus_tag="ACHKBL_003560"
     CDS             3021..3938
                     /gene="oxyR"
                     /locus_tag="ACHKBL_003560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HFO5820377.1"
                     /translation="MNIRDLEYLVALAEHRHFRRAADSCHVSQPTLSGQIRKLEDELG
                     VMLLERTSRKVLFTQAGMLLVDQARTVLREVKVLKEMASQQGETMSGPLHIGLIPTVG
                     PYLLPHIIPMLHQTFPKLEMYLHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPL
                     FDEPMLLAIYEDHPWANRECVPMADLAGEKLLMLEDGHCLRDQAMGFCFEAGADEDTH
                     FRATSLETLRNMVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPRRTIGLVYRPGS
                     PLRSRYEQLAEAIRARMDGHFDKVLKQAV"
     gene            complement(3921..5321)
                     /gene="sthA"
                     /locus_tag="ACHKBL_003561"
     CDS             complement(3921..5321)
                     /gene="sthA"
                     /locus_tag="ACHKBL_003561"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003957 - NAD(P)+ transhydrogenase
                     (Si-specific) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HFO5820378.1"
                     /translation="MPHSYDYDAIVIGSGPGGEGAAMGLVKQGARVAVIERYQNVGGG
                     CTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQ
                     GFYERNHCEILQGNARFVDEHTLALDCPDGSVETLTAEKFVIACGSRPYHPTDVDFTH
                     PRIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQE
                     MSDSLSYHFWNSGVVIRHNEEYEKIEGCDDGVIMHLKSGKKLKADCLLYANGRTGNTD
                     SLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALV
                     KGEATAHLIEDIPTGIYTIPEISSVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMN
                     VGTLKILFHRETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTM
                     AEAYRVAALNGLNRLF"
     gene            5653..6303
                     /gene="fabR"
                     /locus_tag="ACHKBL_003562"
     CDS             5653..6303
                     /gene="fabR"
                     /locus_tag="ACHKBL_003562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HFO5820379.1"
                     /translation="MMGVRAQQKEKTRRSLVEAAFSQLSAERSFASLSLREVAREAGI
                     APTSFYRHFRDVDELGLTMVDESGLMLRQLMRQARQRIAKGGSVIRTSVSTFMEFIGN
                     NPNAFRLLLRERSGTSAAFRAAVAREIQHFIAELADYLELENHMPRAFTEAQAEAMVT
                     IVFSAGAEALDVGVEQRRQLEERLVLQLRMISKGAYYWYRREQEKTAIIPGNVKDE"
     gene            6303..6662
                     /gene="yijD"
                     /locus_tag="ACHKBL_003563"
     CDS             6303..6662
                     /gene="yijD"
                     /locus_tag="ACHKBL_003563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YijD family membrane protein"
                     /protein_id="HFO5820380.1"
                     /translation="MKQANQDRGTLLLALVAGLSINGTFAALFSSIVPFSVFPIISLL
                     LTVYCLHQRYLNRTMPVGLPGLAAACFILGVLLYSTVVRAEYPDIGSNFFPAVLSVIM
                     VFWIGAKMRNRKQEVAE"
     gene            complement(6702..7802)
                     /gene="trmA"
                     /locus_tag="ACHKBL_003564"
     CDS             complement(6702..7802)
                     /gene="trmA"
                     /locus_tag="ACHKBL_003564"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030697 - tRNA
                     (uracil(54)-C5)-methyltransferase activity, S-adenosyl
                     methionine-dependent [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HFO5820381.1"
                     /translation="MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHY
                     RMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAASELINQLMTAMIAGVRNNPVLR
                     HKLFQIDYLTTLSNQAVVSLLYHKKLDDEWRQEAEALRDALRAQNLNVHLIGRATKTK
                     IELDQDYIDERLPVAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELY
                     CGNGNFSLALARNFDRVLATEIAKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNG
                     VREFNRLQGIDLKSYQCETIFVDPPRSGLDSETEKMVQAYPRILYISCNPETLCKNLE
                     TLSQTHKVERLALFDQFPYTHHMECGVLLTAK"
     regulatory      7916..8106
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            8171..10054
                     /gene="btuB"
                     /locus_tag="ACHKBL_003565"
     CDS             8171..10054
                     /gene="btuB"
                     /locus_tag="ACHKBL_003565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015420 - ABC-type vitamin B12 transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015889 - cobalamin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
                     /protein_id="HFO5820382.1"
                     /translation="MIKKASLLTACSVTAFSAWAQDTSPDTLVVTANRFQQPVNTVLA
                     PTDIVTREDIQRWQSKDLNDVMSHLPGVNISQTGGMGTSSSLYVRGTESRHVLVLIDG
                     VPMARAGIDNAIDISQFPVSLVQRVEYIRGPRSAVYGSGAIGGVVNIITMSNDEKAQI
                     NAGMGTNGYQAYDGVINKRFGDTMVTAAGAYQTTKGFNIQPGSPYSGDSDRDGYRNKL
                     FWGGLQHKFNDNFSGFFRGYSYTANNDYDQGSMYGSATGNEEAQNYNQSWDTGLRYNS
                     GIYSSQLIANYQRLKNYNYTNNLGRYAGDATLDDMEQRYIQWGNSIEVGHGAISGGID
                     WKQEKLTSSSITKSDDYERDTTGLYLTGQQQISNVTLEASGREDHDEQFGWHGTWQTA
                     AGWEFIDGYQATLSYGTSFLSPSLGQQYGAARFASIYGPGIAANPNLKPEESKQWEIG
                     IEGVTGTLDWRLSGYRYEIQNLIDYKSINNVNQYINVKSATIKGLEWTGNITTGPVEH
                     RLTLQYVDPRDDETDKVLYRRAKQQVKYELTGQAYGFEWDVSYQYIGQRYDNAYDETS
                     GSSHTVKLGGVSLWDLAVAYPVTSHLTVRGKIANLFDKDYETVYGYQTAGREYTLSGS
                     YTF"
     gene            9999..10856
                     /gene="murI"
                     /locus_tag="ACHKBL_003566"
     CDS             9999..10856
                     /gene="murI"
                     /locus_tag="ACHKBL_003566"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HFO5820383.1"
                     /translation="MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLL
                     PDLHYIYAFDNVAFPYGEKSEEFIVERVVAIVTAVQERYPLALAVVACNTASTVSLPA
                     LREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
                     GSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVL
                     PEGTRLVDSGAAIARRTAWLLEHEAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFE
                     TLEKLAVLG"
BASE COUNT         2601 a         2579 c         2939 g         2962 t
ORIGIN      
        1 ggagtttgtt atgtgattat ttaacgtgtt gttttaacga tgatagctaa ttattcaggt
       61 ggtttttgag ggtgtttttt ctcctcggta gaaattttta tttttaatgt gatcatggtt
      121 gaataatcgc tctttctctc ttgtatggta gtaggcgcaa aattaagttt gaaacgtcta
      181 tttacaacac aggagattgc aataatgaaa attattgctg cagatgtttt tgttacttgc
      241 ccagggcgta atttcgttac gcttaaaata accactgcaa gcggactttg tggattagga
      301 gatgccacgt taaatggtcg cgaattgtcg gtcgcttctt atctgaaaga tcatctttgt
      361 ccacaactaa ttggccgcga tgccagccgt atcgaagata tctggcaatt cttttataaa
      421 ggtgcttact ggcgacgtgg ccccgtcacg atgtcagcca tttctgccat tgatatggcg
      481 ttgtgggata tcaaagcaaa agcggcaaat atgccattgt atcaactgct tggtggtgct
      541 tctcgtgaag gcgttatggt ttattgccac accaccgggc gtacgattga tgaagtcctt
      601 gaagactatg caaagcacca gcagatgggc tttaaggcga ttcgtgtgca gtgtggtgta
      661 ccgggtatgc aaaccaccta tggtctggcg aagggcaaag gactggcgta tgaaccagca
      721 accaaaggcc tgtggccaga agagcagttg tggtcttcag aaaagtatct cgattttacg
      781 ccgaagttat ttgaagcggt gcgtaataag ttcggtttta acgaacatct gttgcatgac
      841 atgcaccatc gcctgacccc catcgaagcg gcgcgctttg ggaagagcat tgaagattac
      901 cgcctgttct ggatggaaga tccgacgcct gccgagaatc aggaatgttt tcgtctgatt
      961 cgtcagcata ccgtgacgcc gattgcggtc ggtgaagtgt ttaacagtat ctgggactgc
     1021 aagcagttaa tcgaagagca gctgattgac tatattcgca cgacgattac ccatgcaggc
     1081 ggcattaccg ggatgcgacg gattgccgat tttgcatcgc tttatcaggt acgtaccggt
     1141 tcgcatggcc cttcagattt atcgcctgtt tgccatgcgg cggcgctgca ttttgacctc
     1201 tgggtgccaa actttggcgt tcaggagtac atgggctatt ccgaacaaat gcttgaagtc
     1261 ttcccgcata gctggcgctt cgatgaaggt tatatgcacc caggcgatga accggggctg
     1321 ggtatttctt tcgatgagaa actggcggcg aaatatccgt atgacccggc gtatctcccc
     1381 gtcgcacgac tggaagatgg cacattgtgg aactggtaat agcggttcgc ttaattattt
     1441 tctgaatatt catcaaatcg attaattccg ctcaaggagc taaaaatgga cgttgatgtg
     1501 tcaaccagtg ttgcgggtaa taaaccgcaa cggattcgtc gtattcagac agtgaccctg
     1561 gttttattat ttatggcggg gatcgttaat tttctcgacc gttcgtcatt gagcgtggca
     1621 ggtgaagcaa ttcgtggcga gttaggatta tccgctaccg agtttggtgt tttgctttct
     1681 gcattttctc tgtcttatgg tttttcacaa cttccttccg gtattttgct agaccgcttt
     1741 ggcccacgaa tagtgttagg cgcaggctta atattctggt cattaatgca ggcattaaca
     1801 ggaatggtta attcttttag ccactttatt ttaatgcgta tcggtctggg tattggcgaa
     1861 gcgccattta tgcctgcggg agtaaagtcg atcaccgact ggtatgcgca aaaagaacgc
     1921 ggcacggcgc tggggatttt taactcatct accgttatcg gtcaggctat cgcgcctcct
     1981 gctcttgtat tgatgcaact ggcatggggc tggcggacga tgtttgtcat catcggcgta
     2041 gcagggattc tggttgggat ctgttggtac gcgtggtatc gcaaccgggc gcagtttgtc
     2101 ctgaccgagg aagaacggac atatctctcc gcgccggtta aaccgcgtcc acagttacaa
     2161 tttagcgagt ggctggcgct gtttaagcat cggacaacct gggggatgat tttaggtttc
     2221 tctggcgtca attataccgg ttggctctac atcgcgtggt tacccggtta tttgcaggcc
     2281 gaacaaggtt tcagtctggc gaaaaccggt tgggtggcgg caattccttt cctcgcggcg
     2341 gcggtcggga tgtgggttaa cggtattgtt gtcgatcggc tggcgaaaaa aggctatgac
     2401 ctggcgaaga cgcgtaaaac ggcgattgtc tgcggtttga tgatgtcggc attaggcacg
     2461 ttgctggtcg tgcaatcttc ctcgccagcc caggcggtag cgtttatctc aatggcgctg
     2521 ttctgcgtgc atttcgcggg aacatctgca tgggggctgg tgcaggtgat ggtgtcagaa
     2581 acaaaagtgg cttccatcgc cgggattcaa aactttggca gttttgtttt tgcttccttt
     2641 gctccgatcg taaccggttg ggtcgtggat accacacact cgtttaatct ggcgctggtt
     2701 atcgcggcct gtgtgacgtt caccggggcg ctgtgttact tctttattgt caaagatcgg
     2761 attgagtaac cgttactatc aggctctctt gctgtgggcc tgtagaataa aaaaaagcgg
     2821 atcctggaga tccgcaaaag ttcacgttgg ctttagttat tcgagttgag aaactctcga
     2881 aacgggcagt gacttcaagg gttaaaagag gtgccgctcc gtttctgtga gcaattatca
     2941 gtcagaatgc ttgataggga taatcgttcg ttgctatgct acctatcgcc gcgaactatc
     3001 gtggcaatgg aggatggata atgaatattc gtgatcttga gtacctggtg gcattggccg
     3061 aacaccgcca ttttcggcgt gcggcagatt cctgccacgt tagccagccg acgcttagcg
     3121 ggcaaattcg taagctggaa gatgagttgg gtgtgatgtt gctggagcgg accagccgta
     3181 aagtgttatt cacccaggcg ggaatgctgc tggtggatca ggcgcgtacc gtgctgcgtg
     3241 aggtgaaagt ccttaaagag atggcaagcc agcagggcga gacgatgtcc ggaccgctgc
     3301 atattggtct gattcccacg gttggaccgt acctgctacc gcatattatc ccgatgctgc
     3361 accagacctt tccaaagctg gaaatgtatc tgcatgaagc acagacccac cagttactgg
     3421 cgcaactgga cagcggcaaa ctcgattgcg tgatcctcgc gctggtgaaa gagagcgaag
     3481 cattcattga agtgccgttg tttgatgagc caatgttgct ggctatctat gaagaccatc
     3541 cgtgggcgaa ccgcgaatgc gtaccgatgg ccgatctggc aggggaaaaa ctgctgatgc
     3601 tggaagatgg tcactgtttg cgcgatcagg caatgggttt ctgttttgaa gccggggcgg
     3661 atgaagatac acacttccgc gcgaccagcc tggaaactct gcgcaacatg gtggcggcag
     3721 gtagcgggat cactttactg cctgcgctgg ctgtgccgcc ggaacgcaaa cgtgatgggg
     3781 ttgtttatct gccatgcatt aagccggaac cacgccgcac tattggcctg gtttatcgtc
     3841 ctggctcacc actgcgcagc cgctatgagc agctggcaga ggccatccgc gcaagaatgg
     3901 atggccattt cgataaagtg ttaaaacagg cggtttaagc catttagcgc agctacccga
     3961 taggcttccg ccatcgtcgg gtagttaaag gtggtgttga cgaagtactc aatagtgttg
     4021 ccgccacctt tctgttccat aatcgcctga ccgatatgaa taatttcggc agcgcgctcg
     4081 ccaaagcagt gaatacccag aatctctttt gtttcccgat ggaacaaaat tttcagcgtg
     4141 cccacgttca tgccgacgat ttgtgcacgt gccagatgtt taaactgggc gcggcccact
     4201 tcatatggca ctttcatcgc ggtcagctgc tgttcggttt tgcccacaga gctgatttcc
     4261 gggatggtgt aaataccggt agggatatct tcaatcagat gtgcggtggc ttcacctttc
     4321 accagcgcct gcgcggcaat gcgcccctgg tcataggccg ccgacgccag gctcggataa
     4381 ccaatcacgt cgcccaccgc gtaaacgtgc ggctgtgcgg tctgatacat gctgttgacc
     4441 ttcagctgtc cgcggctgtc agtttccagc ccaatgttct gtaacgccag cgaatcggta
     4501 ttaccggtgc gaccgttggc atagagcagg cagtcagctt tcagtttttt acccgacttc
     4561 aaatgcatga tcacaccgtc gtcacagcct tcgatcttct cgtactcttc gttgtgacga
     4621 atcactacgc cactgttcca gaagtgatag gagagagaat ctgacatctc ttgatcgaga
     4681 aatgccagca ggcgatcgcg ggtgttgatc agatccactt ttacatccat accgcggaag
     4741 atcgacgcat attcacagcc gatcactcca gcaccataga taagtacatg gcgcggttcg
     4801 tggtgcatgc taagaattga gtcgctgtcg taaatgcgtg gatgcgtgaa atcaacatct
     4861 gttggatgat atggacgaga gccgcaagca ataacaaatt tttcagcggt tagtgtctcg
     4921 acgctgccgt ccgggcaatc cagcgccagc gtgtgctcgt caacaaagcg agcgttcccc
     4981 tgcaatattt cacagtgatt acgttcgtaa aatccctgac gcatgcgcgt ttgttgatta
     5041 atcacgttat cggcatggtt aaggatatcg gcaaaagaag agcggagcag tcgcgaatgg
     5101 tcgctgtaaa gtgggttttg attaaattct ataatgcggc tgacggcgtg acggagagct
     5161 ttcgacggga tggtgcccca gtgggtgcaa ccgccgccaa cattttgata acgctcgata
     5221 actgcgacgc gcgcaccttg cttaaccagg cccattgcag cgccttcgcc gccggggccg
     5281 gaacctatta ctatggcatc gtaatcgtag gaatgtggca tggtagggct tacctgttct
     5341 tatacataaa agcaacagaa tggtaacatt ttatcgcggg taagccaatt gatccccgtc
     5401 atttatctgg ctatatcgtg agcggccttt gctttgtctg tttcttactt ttgacctgaa
     5461 cgttttattg gatttttatc gacgatactc tccgtttaag cggcaggttt ccgttgtacg
     5521 taaaagaacc ggccaaagaa ttgcagtaaa tatgttttat tgcgttaccg ttcattcaca
     5581 atactggagc aatccagtat gttcattctc tggtatagtg ccagcagtac ttttggcaag
     5641 gattcagaca tcgtgatggg cgtaagagcg caacaaaaag aaaaaacccg ccgttcgctg
     5701 gtggaagccg catttagcca attaagtgct gaacgcagct tcgccagcct gagtttgcgt
     5761 gaagtggcgc gtgaagcggg cattgctccc acctcttttt atcggcattt ccgcgacgta
     5821 gacgaactgg gtctgaccat ggttgatgag agcggtttaa tgctacgcca actcatgcgt
     5881 caggcgcgtc agcgtatcgc caaaggcggg agtgtgatcc gcacctcggt ctccacattt
     5941 atggagttca tcggtaataa tcctaacgcc ttccggttat tattgcggga acgctccggc
     6001 acctccgctg cgtttcgtgc cgccgttgcg cgtgaaattc agcacttcat tgcggaactt
     6061 gcggactatc tggaactcga aaaccatatg ccgcgtgcgt ttactgaagc gcaagccgaa
     6121 gcaatggtga cgattgtctt cagtgcgggg gccgaggcgt tggatgtcgg cgtcgaacaa
     6181 cgtcggcaat tagaagagcg actggtactg caactgcgaa tgatttctaa aggggcttat
     6241 tactggtatc gccgtgaaca agagaaaacc gcaattattc cgggaaatgt gaaggacgag
     6301 taatgaaaca agcaaatcaa gatagaggta cgctgctgct ggcgttggtt gctggcttat
     6361 cgattaatgg tactttcgca gcgctgttta gctccattgt gccattttct gtattcccga
     6421 ttatttccct gttgctgacg gtttactgcc tgcatcaacg ttatcttaat cgcaccatgc
     6481 cggtaggctt gccgggtctg gcagctgcct gttttattct cggcgtactg ctgtacagca
     6541 cggtagttcg tgcggaatat ccggatatcg gctctaactt cttcccggca gtactctccg
     6601 tcattatggt gttctggatt ggcgcgaaga tgcgtaaccg taagcaggaa gttgctgagt
     6661 aatcggtatc atgccggatg ctggtgcatc cggcatgggt tttacttcgc ggtcagtaat
     6721 acgccgcact ccatatggtg cgtgtagggg aactgatcaa acagagccag acgttcgacc
     6781 ttgtgcgtct ggcttaatgt ttccagattc ttgcataacg tttccggatt gcaggagata
     6841 tacagaatac gtggatacgc ctgcaccatt ttctcggttt cactgtccag accgctgcgc
     6901 ggtgggtcga caaaaatggt ttcgcactga taactcttta agtcgattcc ttgcaggcgg
     6961 ttaaactcgc gtacgccatt catcgcctga gtaaattctt ctgccgccat acgaataatt
     7021 tgtacgttat caatatggtt agcggcaatg ttgtattgcg cggcagcgac cgacggctta
     7081 gcgatttcgg tggctaatac ccgatcaaaa ttgcgcgcca gtgctaatga aaagttaccg
     7141 ttgccgcagt acagctccag caaatcgcct tttgagcctt tggttacgtc cagcgcccat
     7201 tccagcatct gaatattcat cgccgcgttc ggctgggtaa agctgttttc tacctgacgg
     7261 tagatcatct ctttccctgc gaccggcaga cgttcatcga tgtaatcctg gtccagctcg
     7321 attttggttt tcgttgcccg accaatcaga tgcacattca gattctgcgc tcgcagtgca
     7381 tcgcgcaggg cttccgcttc ctgacgccac tcatcatcca gcttcttatg gtacagcagg
     7441 gaaaccaccg cctgattact cagcgtggtg aggtaatcaa tctggaacaa cttgtggcgc
     7501 agaacgggat tattacgcac acccgcaatc atcgccgtca tcaactggtt gataagttca
     7561 ctggcggcgg ggaagctatc cacgcggatg cggcttttgg tttgttgatc gaaaatgatg
     7621 tgatacaggt catcgccatc gtgccagatg cggaactccg cacgcatccg gtaatgactg
     7681 accggcgagc gaaacacttc cggaaccagg tcagaaaacg gtgccatcat actttgcaaa
     7741 cgtaccactt tttcggctaa ctgcgcttca tactgttctg ttggaaggtg ttcgggggtc
     7801 atgatgtatc ctgaaaaatt aaagtacgcg gcgattgtag ggattgctca tcagatgtcc
     7861 agatcttgat gaattcctat ttgtgagcta cgtctggaca gtaacttgtt acaacctgta
     7921 gcatccactt gccggtcctg tgagttaata gggaatccag tgcgaatctg gagctgacgc
     7981 gcagcggtaa ggaaaggtgc gatgattgcg ttatgcggac actgccatcc ggtgggaagt
     8041 catcatctct tagtatctta gatacccctc caagcccgaa gacctgccgg ctaacgtcgc
     8101 atctggttct catcatcgcg taatattgat gaaacctgcg gcatccttct tctattgtgg
     8161 atgctttaca atgattaaaa aagcttcgct gctgacggcg tgttctgtca cagccttttc
     8221 cgcttgggca caggatacca gcccggatac tctcgttgtt actgctaacc gttttcagca
     8281 gccagtaaat actgtccttg cacctactga tattgttacc cgtgaggata ttcaacgctg
     8341 gcagtcgaaa gacctcaacg atgttatgag tcatctcccc ggtgtaaata tctctcaaac
     8401 tggtggaatg gggacgagtt cttctcttta tgttcgaggc actgaatcgc gccatgtttt
     8461 agtgttgatt gacggtgtgc cgatggcccg tgccggtatc gacaatgcaa ttgatattag
     8521 ccagttcccg gtatcacttg tacagcgtgt tgaatatatt cgtgggccgc gttctgccgt
     8581 ttatggttct ggtgccatcg gaggtgtggt gaacattatt acaatgagta atgatgagaa
     8641 agctcagatc aacgcaggaa tgggtacaaa tggttatcaa gcctatgatg gggtaataaa
     8701 taaacgtttt ggcgatacga tggtaacagc agcaggtgct taccagacga ccaaaggttt
     8761 taacattcag cctggctcac catacagtgg tgatagcgat cgcgatggat atcgcaacaa
     8821 acttttctgg ggggggctgc agcataagtt taacgataac ttctcggggt tcttccgtgg
     8881 ttacagctac acagctaata atgattacga ccagggctcg atgtatggtt ctgctactgg
     8941 caacgaagaa gctcagaatt acaaccagtc ctgggatact ggcctacgct ataactccgg
     9001 catctattcg tctcagttaa ttgctaacta tcaacgactc aagaattata actacacaaa
     9061 taatcttggt cgttatgcgg gagatgccac cctggatgac atggaacagc gttatattca
     9121 gtggggtaat agcattgagg tagggcatgg tgcaattagc ggcgggatcg attggaaaca
     9181 ggaaaaactg acgtccagca gcattacaaa atcagatgat tacgaacgcg ataccaccgg
     9241 gttatacctg actggacagc aacagatttc caatgtgact ttggaagctt ctggccgaga
     9301 agatcatgat gaacagtttg gctggcatgg tacctggcag acagctgctg gctgggagtt
     9361 tattgatggc taccaggcaa cactttctta cggtaccagt tttctttcgc cttctcttgg
     9421 acagcaatac ggggctgctc gctttgcttc aatttatggt cctggtattg cagccaaccc
     9481 aaatttgaag ccagaagaat ctaaacagtg ggagattgga attgaggggg taactgggac
     9541 tctggattgg cggctctcag gatatcgtta tgaaattcag aacctgattg attataaaag
     9601 cataaataat gtcaaccagt acattaatgt gaaatcagcc acgattaaag ggctggaatg
     9661 gacgggaaat attacaaccg gtcctgttga acatcgcttg acgttacaat atgttgaccc
     9721 gcgcgatgat gaaacagata aagtgctgta ccgtcgggca aaacagcagg ttaagtacga
     9781 gttgactgga caagcttatg gtttcgaatg ggacgtatct taccaatata taggtcaacg
     9841 atacgacaac gcttatgacg agacctctgg ctcatcacat acagttaaat taggcggtgt
     9901 gagcttgtgg gatcttgcgg ttgcgtatcc ggtcacctct catctaacag ttcgtggtaa
     9961 aatagccaac ctgttcgaca aagattatga gacagtctat ggctaccaaa ctgcaggacg
    10021 ggaatacacc ttgtctggca gctacacctt ctgaaccacg tcccaccgtg ctggtgtttg
    10081 actccggcgt cggtgggttg tcggtctatg acgagatccg gcatctctta ccggatctcc
    10141 attacattta tgctttcgat aacgtcgctt tcccgtatgg cgaaaaaagc gaagagttta
    10201 ttgttgagcg agtggtggca attgtcaccg cagtgcaaga acgttatccc cttgcgctgg
    10261 ctgtggtcgc ttgcaacacc gccagtaccg tttcacttcc tgcattacgc gaaaagttcg
    10321 acttcccggt tgttggtgtc gtgccggcga ttaaacctgc tgcacgtctg acggcaaatg
    10381 gcattgtcgg attactggca acccgcggaa ctgttaaacg ttcttatact catgagctga
    10441 tcgcgcgttt cgctaatgaa tgccagatag aaatgctggg ctcggcagag atggtcgagt
    10501 tggctgaagc gaagctgcat ggcgaagatg tttctctgga tgcgctaaaa cgtatcttgc
    10561 gtccgtggtt aagaatgaaa gagccgccag ataccgttgt actggggtgt acccatttcc
    10621 ctctactaca agaagaactg ttacaggtgc tgccagaggg aacccggctg gtggattctg
    10681 gcgcagcgat tgctcgccga acggcctggc tgttagaaca tgaagccccg gatgcaaaat
    10741 ctgccgatgc gaatattgcc ttttgtatgg caatgacgcc agaagctgaa caattattgc
    10801 ccgttttaca gcgttacggc ttcgaaacgc tcgaaaaact ggcagtttta ggctgatttg
    10861 gttgaatgtt gcgcggtcag aaaattattt taaatttcct cttgtcaggc cggaataact
    10921 ccctataatg cgccaccact gacacggaac aacggcaaac acgccgccgg gtcagcgggg
    10981 ttctcctgag aatctcaaca gagaaaagca aaaaaatgct tgactctgta gcgggaaagc
    11041 gtattatgca caccccgcgc cgctgagaaa aagcgaagcg g
//
LOCUS       DAZFLD010000156        11047 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.157, whole genome shotgun sequence.
ACCESSION   DAZFLD010000156 DAZFLD010000000
VERSION     DAZFLD010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11047)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11047)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11047
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.157"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            404..784
                     /gene="atpI"
                     /locus_tag="ACHKBL_003567"
     CDS             404..784
                     /gene="atpI"
                     /locus_tag="ACHKBL_003567"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HFO5820384.1"
                     /translation="MSVSLVSRNVARKLLLVQLLVVIASGLLFSLKDPFWGVSAISGG
                     LAVFLPNVLFMIFAWRHQAHTPAKGRVAWTFAFGEAFKVLAMLVLLVVALAVLKAVFL
                     PLIVTWVLVLVVQILAPAVINNKG"
     gene            793..1608
                     /gene="atpB"
                     /locus_tag="ACHKBL_003568"
     CDS             793..1608
                     /gene="atpB"
                     /locus_tag="ACHKBL_003568"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HFO5820385.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPHNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYSIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            1655..1894
                     /gene="atpE"
                     /locus_tag="ACHKBL_003569"
     CDS             1655..1894
                     /gene="atpE"
                     /locus_tag="ACHKBL_003569"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HFO5820386.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            1956..2426
                     /gene="atpF"
                     /locus_tag="ACHKBL_003570"
     CDS             1956..2426
                     /gene="atpF"
                     /locus_tag="ACHKBL_003570"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HFO5820387.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAHKDLDLAKASATDQLKKAKAEAQVIIEQANKRRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            2441..2974
                     /gene="atpH"
                     /locus_tag="ACHKBL_003571"
     CDS             2441..2974
                     /gene="atpH"
                     /locus_tag="ACHKBL_003571"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HFO5820388.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLAFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRAGDMVI
                     DGSVRGRLERLADVLQS"
     gene            2987..4528
                     /gene="atpA"
                     /locus_tag="ACHKBL_003572"
     CDS             2987..4528
                     /gene="atpA"
                     /locus_tag="ACHKBL_003572"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HFO5820389.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLADVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            4579..5442
                     /gene="atpG"
                     /locus_tag="ACHKBL_003573"
     CDS             4579..5442
                     /gene="atpG"
                     /locus_tag="ACHKBL_003573"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HFO5820390.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YAETMRKVIGHLAHGNLEYKHPYLEDRDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKGVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            5469..6851
                     /gene="atpD"
                     /locus_tag="ACHKBL_003574"
     CDS             5469..6851
                     /gene="atpD"
                     /locus_tag="ACHKBL_003574"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HFO5820391.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            6872..7291
                     /gene="atpC"
                     /locus_tag="ACHKBL_003575"
     CDS             6872..7291
                     /gene="atpC"
                     /locus_tag="ACHKBL_003575"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HFO5820392.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQPGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            7640..9010
                     /gene="glmU"
                     /locus_tag="ACHKBL_003576"
     CDS             7640..9010
                     /gene="glmU"
                     /locus_tag="ACHKBL_003576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HFO5820393.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML
                     YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQEINTGILIANGADMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLRDPARFDLRGTLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGTGCVIKNSVIGDDCEISPYTVVEDANLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGSKAGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQSQKEGWR
                     RPVKKK"
     gene            9172..11001
                     /gene="glmS"
                     /locus_tag="ACHKBL_003577"
     CDS             9172..11001
                     /gene="glmS"
                     /locus_tag="ACHKBL_003577"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HFO5820394.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLHGGTGIAHTRWATHGEPSEANAHPHVSEHIVVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
BASE COUNT         2653 a         2729 c         3017 g         2648 t
ORIGIN      
        1 ttattaaaaa tgtcaatggg tggtttttgt tgtgtaaatg tcatttatta aaacagtatc
       61 tgtttttaga ctgaaatatc ataaacttgc aaaggcatca tttgccaagt aaataaatat
      121 gctgtgcgcg aacatgctca atatgtgatc tgaagcacgc tttatcacca gtgtttacgc
      181 gttatttaca gtttttcatg atcgaacagg gtttgcagaa aagtcgcaat tgtatgcact
      241 ggaaaaatat ttaaacattt attcaccttt tggctactta ttgtttgaaa tcacgggggc
      301 gcaccgtata atttgaccgc tttttgatgc ttgactctaa gccttaaaga aagttttata
      361 cgacacgcgg catacctcga agggagcagg agtgaaaaac gtgatgtctg tgtcgctcgt
      421 gagtcgaaac gttgctcgga agcttctgct cgttcagtta ctggtggtga tagcaagtgg
      481 attgctgttc agcctcaaag accccttctg gggcgtctct gcaataagcg ggggcctggc
      541 agtttttctg cctaacgttt tgtttatgat atttgcctgg cgtcaccagg cgcatacacc
      601 agcgaaaggc cgggtggcct ggacattcgc atttggcgaa gctttcaaag ttctggcgat
      661 gttggtgtta ctggtggtgg cgttggcggt tttaaaggcg gtattcttgc cgctgatcgt
      721 tacgtgggtt ttggtgctgg tggttcagat actggcaccg gctgtaatta acaacaaagg
      781 gtaaaaggca tcatggcttc agaaaatatg acgccgcagg attacatagg acaccacctg
      841 aacaaccttc agctggacct gcgtacattc tcgctggtgg atccacataa ccccccagcc
      901 accttctgga caatcaatat tgactccatg ttcttctcgg tggtgctggg tctgttgttc
      961 ctggttttat tccgtagcgt agccaaaaag gcgaccagcg gtgtgccagg taagtttcag
     1021 accgcgattg agctggtgat cggctttgtt aatggtagcg tgaaagacat gtaccatggc
     1081 aaaagcaagc tgattgctcc gctggccctg acgatcttcg tctgggtatt cctgatgaac
     1141 ttgatggatt tactgcctat cgacctgctg ccgtacattg ctgaacatgt actgggtctg
     1201 cctgcactgc gtgtggttcc gtctgcggac gtgaacgtaa cgctgtctat ggcactgggt
     1261 gtatttatcc tgattctgtt ctacagcatc aaaatgaaag gcatcggcgg cttcacgaaa
     1321 gagttgacgc tgcagccgtt caatcactgg gcgttcattc ctgtcaactt aatccttgaa
     1381 ggggtaagcc tgctgtccaa accagtttca ctcggtttgc gactgttcgg taacatgtat
     1441 gccggtgagc tgattttcat tctgattgct ggtctgttgc cgtggtggtc acagtggatt
     1501 ctgaatgtgc cgtgggccat tttccacatc ctgatcatta cgctgcaagc cttcatcttc
     1561 atggttctga cgatcgtcta tctgtcgatg gcgtctgaag aacattaatt taccaacact
     1621 actacgtttt aactgaaaca aactggagac tgtcatggaa aacctgaata tggatctgct
     1681 gtacatggct gccgctgtga tgatgggtct ggcggcaatc ggtgctgcga tcggtatcgg
     1741 catcctcggg ggtaaattcc tggaaggcgc agcgcgtcaa cctgatctga ttcctctgct
     1801 gcgtactcag ttctttatcg ttatgggtct ggtggatgct atcccgatga tcgctgtagg
     1861 tctgggtctg tacgtgatgt tcgctgtcgc gtagtaagcg ttgcttttat ttaaagagca
     1921 atatcagaac gttaactaaa tagaggcatt gtgctgtgaa tcttaacgca acaatcctcg
     1981 gccaggccat cgcgtttgtc ctgttcgttc tgttctgcat gaagtacgta tggccgccat
     2041 taatggcagc catcgaaaaa cgtcaaaaag aaattgctga cggccttgct tccgcagaac
     2101 gagcacataa ggaccttgac cttgcaaagg ccagcgcgac cgaccagctg aaaaaagcga
     2161 aagcggaagc ccaggtaatc atcgagcagg ctaacaaacg ccgctcgcag attctggacg
     2221 aagcgaaagc tgaggcagaa caggaacgta ctaaaatcgt ggcccaggcg caggcggaaa
     2281 ttgaagccga gcgtaaacgt gcccgtgaag agctgcgtaa gcaagttgct atcctggctg
     2341 ttgctggcgc cgagaagatc atcgaacgtt ccgtggatga agctgctaac agcgacatcg
     2401 tggataaact tgtcgctgaa ctgtaaggag ggaggggctg atgtctgaat ttattacggt
     2461 agctcgcccc tacgccaaag cagcttttga ctttgccgtc gaacaccaaa gtgtagaacg
     2521 ctggcaggac atgctggcgt ttgccgccga ggtaaccaaa aacgaacaaa tggcagagct
     2581 tctctctggc gcgcttgcgc cagaaacgct cgccgagtcg tttatcgcag tttgtggtga
     2641 gcaactggac gaaaacggtc agaacctgat tcgggttatg gctgaaaatg gtcgtcttaa
     2701 cgcgctcccg gatgttctgg agcagtttat tcacctgcgt gccgtgagtg aggctaccgc
     2761 tgaggtagac gtcatttccg ctgccgcact gagtgaacaa cagctcgcga aaatttctgc
     2821 tgcgatggaa aaacgtctgt cacgcaaagt taagctgaat tgcaaaatcg ataagtctgt
     2881 aatggcaggc gttatcatcc gagcgggtga tatggtcatt gatggcagcg tacgcggtcg
     2941 tcttgagcgc cttgcagacg tcttgcagtc ttaaggggac tggagcatgc aactgaattc
     3001 caccgaaatc agcgaactga tcaagcagcg cattgctcag ttcaatgttg tgagtgaagc
     3061 tcacaacgaa ggtactattg tttctgtaag tgacggtgtt atccgcattc acggcctggc
     3121 cgattgtatg cagggtgaaa tgatctccct gccgggtaac cgttacgcta tcgcactgaa
     3181 cctcgagcgc gactctgtag gtgcggttgt tatgggtccg tacgctgacc ttgccgaagg
     3241 catgaaagtt aagtgtactg gccgtatcct ggaagttccg gttggccgtg gcctgctggg
     3301 ccgtgtggtt aacactctgg gtgcaccaat cgacggtaaa ggtccgctgg atcacgacgg
     3361 cttctctgct gtagaagcaa tcgctccggg cgttatcgaa cgtcagtccg tagatcagcc
     3421 ggtacagacc ggttataaag ccgttgactc catgatccca atcggtcgtg gtcagcgtga
     3481 attgatcatc ggtgaccgtc agaccggtaa aaccgcactg gctatcgatg ccatcatcaa
     3541 ccagcgcgat tccggtatca aatgtatcta tgtcgctatc ggccagaaag cgtccaccat
     3601 ttctaacgtg gtacgtaaac tggaagagca cggcgcactg gctaacacca tcgttgtggt
     3661 agcaaccgcg tctgaatccg ctgcactgca atacctggca ccgtacgctg gttgtgcaat
     3721 gggcgaatac ttccgtgacc gcggtgaaga tgcgctgatc atttacgatg acctgtctaa
     3781 acaggctgtt gcttaccgtc agatctccct gctgctccgt cgtccgccag gacgtgaagc
     3841 attcccgggc gacgtattct acctccactc tcgtctgctg gagcgtgctg cgcgtgttaa
     3901 cgccgaatac gttgaagcct tcaccaaagg tgaagtgaaa gggaaaaccg gttctctgac
     3961 tgcgctgccg attatcgaaa ctcaggcggg tgacgtttct gcgttcgttc caaccaacgt
     4021 aatctccatt accgatggtc agatcttcct ggaaaccaac ctgttcaacg ccggtattcg
     4081 tcctgcggtt aacccgggta tttccgtatc ccgtgttggt ggtgcagcac agaccaagat
     4141 catgaaaaaa ctgtccggtg gtatccgtac cgctctggca cagtatcgtg aactggcagc
     4201 gttctctcag tttgcatccg accttgacga tgcaacacgt aagcagcttg accacggtca
     4261 gaaagtgacc gaactgctga agcagaaaca gtatgcgccg atgtctgttg cgcaacagtc
     4321 tctggttctg ttcgcagcag aacgtggtta cctggcggat gtagaactgt cgaaaatcgg
     4381 cagcttcgaa gccgctctgc tggcttacgt cgaccgtgat cacgctccgt tgatgcaaga
     4441 gatcaaccag accggtggct acaacgacga aatcgaaggc aagctgaaag gcatcctcga
     4501 ttccttcaaa gcaacccaat cctggtaacg tctggcggct tgccttaggg caggccgcaa
     4561 ggcattgagg agaagctcat ggccggcgca aaagagatac gtagtaagat cgcaagcgtc
     4621 cagaacacgc aaaagatcac taaagcgatg gagatggtcg ccgcttccaa aatgcgtaaa
     4681 tcgcaggatc gcatggcggc cagccgtcct tatgcagaaa ccatgcgcaa agtgattggt
     4741 caccttgcac acggtaatct ggaatataag cacccttacc tggaagaccg cgacgttaaa
     4801 cgcgtgggct acctggtggt gtcgaccgac cgtggtttgt gcggtggttt gaacattaac
     4861 ctgttcaaaa aactgctggc ggaaatgaag acctggaccg acaaaggcgt tcaatgcgac
     4921 ctcgcaatga tcggctcgaa aggcgtgtcg ttcttcaact ccgtgggcgg caatgttgtt
     4981 gcccaggtca ccggcatggg ggataaccct tccctgtccg aactgatcgg tccggtaaaa
     5041 gtgatgttgc aggcctacga cgaaggccgt ctggacaagc tttacattgt cagcaacaaa
     5101 tttattaaca ccatgtctca ggttccgacc atcagccagc tgctgccgtt accggcatca
     5161 gatgatgatg atctgaaaca taaatcctgg gattacctgt acgaacccga tccgaaggcg
     5221 ttgctggata ccctgctgcg tcgttatgtc gaatctcagg tttatcaggg cgtggttgaa
     5281 aacctggcca gcgagcaggc cgcccgtatg gtggcgatga aagccgcgac cgacaatggc
     5341 ggcagcctga ttaaagagct gcagttggta tacaacaaag ctcgtcaggc cagcattact
     5401 caggaactca ccgagatcgt ctcgggggcc gccgcggttt aaacaggtta tttcgtagag
     5461 gatttaagat ggctactgga aagattgtcc aggtaatcgg cgccgtagtt gacgtcgaat
     5521 tccctcagga tgccgtaccg cgcgtgtacg atgctcttga ggtgcaaaat ggtaatgagc
     5581 gtctggtgct ggaagttcag cagcagctcg gcggcggtat cgtacgtacc atcgcaatgg
     5641 gttcctccga cggtctgcgt cgcggtctgg atgtaaaaga cctcgaacac ccgatcgaag
     5701 tcccggtagg taaagcgact ctgggccgta tcatgaacgt actgggtgaa ccggtcgaca
     5761 tgaaaggcga gatcggtgaa gaagagcgtt gggcgattca ccgcgcagca ccttcctacg
     5821 aagagctgtc aaactctcag gaactgctgg aaaccggtat caaagttatc gacctgatgt
     5881 gtccgttcgc taagggcggt aaagttggtc tgttcggtgg tgcgggtgta ggtaaaaccg
     5941 taaacatgat ggagcttatt cgtaacatcg cgatcgagca ctccggttac tctgtgtttg
     6001 cgggcgtagg tgaacgtact cgtgagggta acgacttcta ccacgaaatg accgactcca
     6061 acgttatcga caaagtatcc ctggtgtatg gccagatgaa cgagccgccg ggaaaccgtc
     6121 tgcgcgttgc tctgaccggt ctgaccatgg ctgagaaatt ccgtgacgaa ggtcgtgacg
     6181 ttctgctgtt cgttgacaac atctatcgtt acaccctggc cggtacggaa gtatccgcac
     6241 tgctgggccg tatgccttca gcggtaggtt atcagccgac cctggcggaa gagatgggcg
     6301 ttctgcagga acgtatcacc tccaccaaaa ccggttctat cacctccgta caggcggtat
     6361 acgtacctgc ggatgacttg actgacccgt ctccggcaac cacctttgcg caccttgacg
     6421 caaccgtggt actgagccgt cagatcgcgt ctctgggtat ctacccggcc gttgacccgc
     6481 tggactccac cagccgtcag ctggacccgc tggtggttgg tcaggaacac tacgacaccg
     6541 cgcgtggcgt tcagtccatc ctgcaacgtt atcaggaact gaaagacatt atcgccatcc
     6601 tgggtatgga tgaactgtct gaagaagaca aactggtggt agcgcgtgct cgtaagatcc
     6661 agcgcttcct gtcccagccg ttcttcgtgg cagaagtatt caccggttct ccgggtaaat
     6721 acgtctccct gaaagacacc atccgtggct ttaaaggcat catggaaggc gaatacgatc
     6781 acctgccgga gcaggcgttc tacatggtcg gttccatcga agaagctgtg gaaaaagcca
     6841 aaaaacttta acgccttaat cggagggtga tatggcaatg acttaccacc tggacgtcgt
     6901 cagtgcagag caacaaatgt tctctggtct ggtcgagaaa atccaggtaa cgggtagcga
     6961 aggtgaactg gggatctatc ctggccacgc accgctgctc accgccatta agcctggtat
     7021 gattcgcatc gtgaaacagc acggtcacga agagtttatc tatctgtctg gcggcattct
     7081 tgaagtgcag cctggcaacg tgaccgttct ggccgacacc gcaattcgcg gccaggatct
     7141 cgacgaagcg cgagccatgg aagcgaaacg taaggctgaa gagcacatta gcagctctca
     7201 cggcgacgta gattacgctc aggcgtctgc ggaactggcc aaagcgatcg cgcagctgcg
     7261 cgttatcgag ttgaccaaaa aagcgatgta acaccggctt gaaaagcaca aaagccagtc
     7321 tggaaacagg ctggcttttt ttacgcgtga cccgtcctga agaacgttca catagatcct
     7381 gctgatataa aaccccctgt tttcccgttt attcattgat cgaaataaga gcaaaaacat
     7441 ccacctgacg cttaaatgaa ggtactgcct taattttctg cagacaaaag gtgtgacgat
     7501 ggtcgaaaat ggcgctttcg tcagcgggga taatccgtta ttgaacaatt tatcctctgt
     7561 ccatttcacg atgaaaaaaa tgtagttttt tcaaggtgaa gcggtttaaa ttcgttctca
     7621 aattacagtc aggacgcgta tgttgaataa tgctatgagc gtagtgatcc ttgccgcagg
     7681 caaaggcacg cgcatgtatt ccgatcttcc gaaagtgctg catacccttg ccgggaaagc
     7741 gatggttcag catgtcattg atgctgcgaa tgaattaggc gcagcgcacg ttcacctggt
     7801 ttacggtcac ggcggcgatc tgcttaaaca ggcgctgaaa gatgacaacc tgaactgggt
     7861 gcttcaggca gaacagctgg gtacaggtca tgcgatgcag caggccgcac ctttctttgc
     7921 cgatgatgaa gacattttaa tgctctacgg cgacgtgccg ctgatctctg tcgaaacact
     7981 ccagcgtctg cgtgatgcta aaccgcaggg tggcattggt ctgctgacgg taaaactgga
     8041 tgatccgacc ggttatggac gtatcacccg tgaaaacggc aaagttaccg gcattgttga
     8101 gcacaaagac gccaccgacg agcagcgtca gattcaggag atcaacaccg gcattctgat
     8161 tgccaacggc gcagatatga aacgctggct ggcgaagctg accaacaata acgctcaggg
     8221 tgaatactac atcaccgaca ttattgcgct ggcgtatcag gaagggcgtg aaatcgtcgc
     8281 cgttcatccg caacgtttaa gcgaagtaga aggcgtgaat aaccgcctgc aactctcccg
     8341 actggagcgc gtttaccagt ctgaacaggc tgaaaaactg ctgttagcag gcgttatgct
     8401 gcgcgatccg gcgcgttttg atctgcgcgg tacgcttact cacgggcgcg atgttgaaat
     8461 tgatactaac gttatcatcg agggcaacgt gactctcggt catcgcgtga aaatcggcac
     8521 cggttgcgtg attaaaaaca gcgtgattgg cgatgattgc gaaatcagtc cgtataccgt
     8581 tgtggaagat gcgaatctgg cagcggcctg taccattggc ccgtttgccc gtctgcgtcc
     8641 tggtgctgag ttgctggaag gtgcacacgt cggtaacttc gttgagatga aaaaagcgcg
     8701 tctgggtaaa ggctcgaaag ccgggcatct gacttatctg ggcgatgcgg aaattggcga
     8761 taacgttaac atcggcgcgg gaaccattac ctgcaactac gacggtgcga ataaatttaa
     8821 gaccattatc ggcgacgatg tgtttgttgg ttccgatact cagctggtgg ccccggtaac
     8881 ggtaggcaaa ggcgcaacca ttgctgcggg tacaactgtg acgcgtaatg tcggcgaaaa
     8941 tgctctggca atcagccgtg tgccgcagtc gcaaaaagaa ggctggcgtc gtccggtaaa
     9001 gaaaaagtaa ttctggctgg gaaatccggt catatgggat gaggagataa cataatctcc
     9061 cgcccacaag cagtaactat aaaaataacc ccactctcta caaggctcgg ggcgcccgaa
     9121 aaaacgggca tacaggttga ccgacaacga tataaatcgg aatcaaaaac tatgtgtgga
     9181 attgttggcg cgatcgcgca acgtgatgta gcagaaatcc ttcttgaagg tttacgtcgt
     9241 ctggaatacc gcggatatga ctctgccggt ctggccgttg ttgatgcaga aggtcatatg
     9301 acccgcctgc gtcgcctcgg taaagtacag atgctggctc aggcagcgga agaacatcct
     9361 ctgcatggcg gcaccggtat tgctcatact cgctgggcga cccacggtga accttcagaa
     9421 gctaacgcgc atccgcatgt ttctgaacac attgtggtgg tgcataacgg catcatcgaa
     9481 aaccatgaac cgctgcgtga agagttaaaa gcgcgtggct ataccttcgt ttctgaaacc
     9541 gacaccgaag tgattgcaca tttggtgaac tgggagctga aacaaggcgg tactctgcgt
     9601 gaggcagttc tgcgtgctat tccgcaactg cgtggtgcgt acggtacagt gatcatggac
     9661 tcccgtcacc cggataccct gctggcggca cgttctggta gcccgctggt gattggtctg
     9721 ggcatgggcg aaaactttat cgcttctgac cagctggcgc tgttgccagt gactcgtcgc
     9781 tttatcttcc ttgaagaggg cgatattgcg gaaatcactc gccgttcggt aaacattttc
     9841 gataaaactg gcgcggaagt aaaacgtcag gatattgaat ccaatctgca atatgacgcg
     9901 ggcgataaag gtatttaccg tcactacatg cagaaagaga tctacgaaca accgaacgcg
     9961 atcaaaaaca cccttaccgg gcgcatcagc cacggtcagg ttgatttaag cgagctggga
    10021 ccgaacgccg acgaactgct gtcgaaggtt gagcatattc agatcctcgc ctgtggtact
    10081 tcttataact ccggtatggt ttcccgctac tggtttgaat cgctggcagg tattccgtgc
    10141 gacgtcgaaa tcgcctctga attccgctat cgcaaatctg ccgtgcgtcg taacagcctg
    10201 atgatcacct tgtcacagtc tggcgaaacc gcggataccc tggcgggcct gcgtctgtcg
    10261 aaagagctgg gttaccttgg ttcgctggca atctgtaacg ttccgggttc ttctctggtg
    10321 cgcgaatccg atctggcgct gatgaccaac gccggtacgg aaatcggcgt ggcatccact
    10381 aaagcattca ccactcagtt aactgtgctg ttgatgctgg tggcgaagct gtctcgcctg
    10441 aaaggtctgg atgcctccat tgaacatgac attgtgcatg gtctgcaggc gctgccgagc
    10501 cgtattgagc agatgctgtc tcaggacaaa cgcattgaag cgctggcaga agatttctct
    10561 gacaaacatc acgcgctgtt cctgggccgt ggcgatcagt acccaatcgc gctggaaggt
    10621 gcattgaagc tgaaagagat ctcttacatt cacgctgaag cctacgctgc gggcgaactg
    10681 aaacacggcc cgctggcgct gattgacgcc gatatgccgg ttatcgtcgt tgcgccgaac
    10741 aacgagttgt tggaaaaact gaaatctaac attgaagaag ttcgcgcgcg tggcggtcag
    10801 ttgtatgtct tcgccgatca ggatgcgggt tttgtgagca gtgacaacat gcacatcatt
    10861 gagatgccgc atgtggaaga ggtgattgca ccaatcttct acaccgttcc gctgcagcta
    10921 ctggcttacc acgttgcgct gatcaaaggc accgacgttg accagccgcg taacctggcg
    10981 aaatcggtta cggttgagta atatctctcg ttgatagaaa agggctgccg accggcggcc
    11041 ctttttt
//
LOCUS       DAZFLD010000157        10863 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.158, whole genome shotgun sequence.
ACCESSION   DAZFLD010000157 DAZFLD010000000
VERSION     DAZFLD010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10863
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.158"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1069
                     /gene="yghO"
                     /locus_tag="ACHKBL_003578"
     CDS             <1..1069
                     /gene="yghO"
                     /locus_tag="ACHKBL_003578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000981816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protein YghO"
                     /protein_id="HFO5820395.1"
                     /translation="PSSLYPDDPNWIPPLFIERSEHLSAKNPGTDHIIWQAWVAKKAG
                     QIVGRITAQIDTLHRERYGEDTGHYGMIDAIDDPQVFAALFGAAEAWLKSQGANKISG
                     PFSLNINQESGLLIEGFDTPPCAMMPHGKPWYAAHIEQLGYHKGIDLLAWWMQRTDLT
                     FSPALKKLMDQVRKKVTIRCINRQRFAEEMQILREIFNSGWQHNWGFVPFTEHEFATM
                     GDQLKYLVPDDMIYIAEIDSAPCAFIVGLPNINEAIADLNGSLFPFGWAKLLWRLKVS
                     GVRTARVPLMGVRDEYQFSRIGPVIALLLIEALRDPFARRKIDALEMSWILETNTGMN
                     NMLERIGAEPYKRYRLYEKQI"
     gene            complement(1076..1840)
                     /gene="glcC"
                     /locus_tag="ACHKBL_003579"
     CDS             complement(1076..1840)
                     /gene="glcC"
                     /locus_tag="ACHKBL_003579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001305094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GlcC"
                     /protein_id="HFO5820396.1"
                     /translation="MKDERRPICEVVAESIERLIIDGVLKVGQPLPSERRLCEKLGFS
                     RSALREGLTVLRGRGIIETAQGRDSRVARLNRVQDASPLIHLFSTQPRTLYDLLDVRA
                     LLEGESARLAATLGTQADFVVITRCYEKMLAASENNKEISLIEHAQLDHAFHLAICQA
                     SHNQVLVFTLQSLTDLMFNSVFASVNNLYHRPQQKKQIDRQHARIYNAVLQRLPHVAQ
                     RAARDHVRTVKKNLHDIELEGHHLIRSAVPLEMKQG"
     gene            2091..3590
                     /gene="glcD"
                     /locus_tag="ACHKBL_003580"
     CDS             2091..3590
                     /gene="glcD"
                     /locus_tag="ACHKBL_003580"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009339 - glycolate oxidase complex
                     [Evidence IEA];
                     GO_function: GO:0003973 - (S)-2-hydroxy-acid oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcD"
                     /protein_id="HFO5820397.1"
                     /translation="MSILYEERLDGALPDVDRTSVLMALREHVPGLEILHTDEEIIPY
                     ECDGLSAYRTRPLLVVLPKQMEQVTAILAVCHRLRVPVVTRGAGTGLSGGALPLEKGV
                     LLVMARFKEILDINPVGRRARVQPGVRNLAISQAVAPHNLYYAPDPSSQIACSIGGNV
                     AENAGGVHCLKYGLTVHNLLKIEVQTLDGEALTLGSDALDSPGFDLLALFTGSEGMLG
                     VTTEVTVKLLPKPPVARVLLASFDSVEKAGLAVGDIIANGIIPGGLEMMDNLSIRAAE
                     DFIHAGYPVDAEAILLCELDGVESDVQEDCERVNDILLKAGATDVRLAQDEAERVRFW
                     AGRKNAFPAVGRISPDYYCMDGTIPRRALPGVLEGIARLSQQYDLRVANVFHAGDGNM
                     HPLILFDANEPGEFARAEELGGKILELCVEVGGSISGEHGIGREKINQMCAQFNSDEI
                     TTFHAVKAAFDPDGLLNPGKNIPTLHRCAEFGAMHVHHGHLPFPELERF"
     gene            3590..4642
                     /gene="glcE"
                     /locus_tag="ACHKBL_003581"
     CDS             3590..4642
                     /gene="glcE"
                     /locus_tag="ACHKBL_003581"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcE"
                     /protein_id="HFO5820398.1"
                     /translation="MLRECDYSQALLEQVNQAISDKTPLVIQGSNSKAFLGRPVTGQT
                     LDVRCHRGIVNYDPTELVITARAGTPLVAIEAALESAGQMLPCEPPHYGEEATWGGMV
                     ACGLAGPRRPWSGSVRDFVLGTRIITGTGKHLRFGGEVMKNVAGYDLSRLMAGSYGCL
                     GVLTEISMKVLPRPRATLSLRREISLQEAMNEIAQWQLQPLPISGLCYFDNALWIRLE
                     GGEGSVKAARELLGGEEVAGQFWQQLREQQLPFFSLPGTLWRISLPSDAPMMDLPGEQ
                     LIDWGGALRWLKSTADDNQIHRIARNAGGHATRFSAGDGGFAPLPAPLFRYHQQLKQQ
                     LDPCGVFNPGRMYAEL"
     gene            4653..5876
                     /gene="glcF"
                     /locus_tag="ACHKBL_003582"
     CDS             4653..5876
                     /gene="glcF"
                     /locus_tag="ACHKBL_003582"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
                     /protein_id="HFO5820399.1"
                     /translation="MQTRLTEEMRQNARALEADSILRACVHCGFCTATCPTYQLLGDE
                     LDGPRGRIYLIKQVLEGNEVTLKTQEHLDRCLTCRNCETTCPSGVRYHNLLDIGRDIV
                     EQKVKRPLPERMLREGLRQVVPRPAVFRALTQVGLVLRPFLPEQVRAKLPAETVKAKP
                     RPPLRHKRRVLMLEGCAQPTLSPNTNAATARVLDRLGISVTPANEAGCCGAVDYHLNA
                     QEKGLARARNNIDAWWPAIEAGVEAILQTASGCGAFVKEYGQMLKNDALYADKARLVS
                     ELAVDLVELLREEPLEKLAIRGDKKLAFHCPCTLQHAQKLNGEVEKVLLRLGFTLTDV
                     PDSHLCCGSAGTYALTHPDLARQLRDNKMNALESGKPEMIVTANIGCQTHLASADRTS
                     VRHWIEIVEQALEKE"
     gene            5881..6285
                     /locus_tag="ACHKBL_003583"
     CDS             5881..6285
                     /locus_tag="ACHKBL_003583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlcG family protein"
                     /protein_id="HFO5820400.1"
                     /translation="MKTKVILSQQMASAIIAAGQEEAQKNNWSVSIAVADDGGHLLAL
                     SRMDDCAPIAAYISQEKARTAALGRRETKGYEEMVNNGRTAFVTAPLLTSLEGGVPVV
                     VDGQIIGAVGVSGLTGAQDAQVAKAAAAVLAK"
     gene            6307..8478
                     /gene="glcB"
                     /locus_tag="ACHKBL_003584"
     CDS             6307..8478
                     /gene="glcB"
                     /locus_tag="ACHKBL_003584"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006097 - glyoxylate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase G"
                     /protein_id="HFO5820401.1"
                     /translation="MSQTITQGRLRINANFKRFVDEEVLPGTGLDTAAFWRNFDEIVH
                     DLAPENRQLLAERDRIQAALDEWHRSNPGPVKDKAAYKSFLRELGYLVPQPERVTVET
                     TGIDSEITSQAGPQLVVPAMNARYALNAANARWGSLYDALYGSDIIPQEGAMVSGYDP
                     QRGEQVIAWVRRFLDESLPLENGSYQDVVAFKVVDKQLRIQLKNGKETTLRTPAQFVG
                     YRGDAAAPSCILLKNNGLHIELQIDANGRIGKDDSAHINDVIVEAAISTILDCEDSVA
                     AVDAEDKILLYRNLLGLMQGTLQEKMEKNGRQIVRKLNDDRQYTAADGSEISLHGRSL
                     LFIRNVGHLMTIPVIWDSEGNEIPEGILDGVMTGAIALYDLKVQKNSRTGSVYIVKPK
                     MHGPHEVAFANKLFTRIETMLGMAPNTLKMGIMDEERRTSLNLRSCIAQARNRVAFIN
                     TGFLDRTGDEMHSVMEAGPMLRKNQMKSTPWIKAYERNNVLSGLFCGLRGKAQIGKGM
                     WAMPDLMADMYSQKGDQLRAGANTAWVPSPTAATLHALHYHQTNVQSVQANIAQTEFN
                     AEFEPLLDDLLTIPVAENANWSAQEIQQELDNNVQGILGYVVRWVEQGIGCSKVPDIH
                     NVALMEDRATLRISSQHIANWLRHGILTKDQVQASLENMAKVVDQQNAGDPAYRPMVE
                     NFANSCAFKAACDLIFLGVKQPNGYTEPLLHAWRLREKENH"
     gene            8834..10516
                     /gene="glcA"
                     /locus_tag="ACHKBL_003585"
     CDS             8834..10516
                     /gene="glcA"
                     /locus_tag="ACHKBL_003585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate permease GlcA"
                     /protein_id="HFO5820402.1"
                     /translation="MVTWTQMYMPMGGLGLSALVALIPIIFFFVALAVLRLKGHVAGA
                     ITLILSILIAIFAFKMPIDMAFAAAGYGFIYGLWPIAWIIVAAVFLYKLTVASGQFDI
                     IRSSVISITDDQRLQVLLIGFSFGALLEGAAGFGAPVAITGALLVGLGFKPLYAAGLC
                     LIANTAPVAFGALGVPILVAGQVTGIDPFDIGAMAGRQLPFLSVLVPFWLVAMMDGWK
                     GVKETWPAALVAGGSFAVTQFFTSNYIGPELPDITSALVSIVSLSLFLKVWRPKNTET
                     AISMGQSAGAMVVNKPSSGGPVPSEYRLGQIIRAWSPFLILTVLVTIWTMKPFKALFA
                     PGGAFYSLVINFQIPHLHQQVLKAAPIVAQPTPMDAVFKFDPLSAGGTAIFIAAIISI
                     FILGVGIKKGIGVFAETLISLKWPILSIGMVLAFAFVTNYSGMSTTLALVLAGTGVMF
                     PFFSPFLGWLGVFLTGSDTSSNALFGSLQSTTAQQINVSDTLLVAANTSGGVTGKMIS
                     PQSIAVACAATGMVGRESELFRYTVKHSLIFASVIGIITLLQAYVFTGMLVS"
     gene            complement(10544..>10863)
                     /locus_tag="ACHKBL_003586"
     CDS             complement(10544..>10863)
                     /locus_tag="ACHKBL_003586"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5820403.1"
                     /translation="IPYSMSDGVFIKRFNKQKSNNYSQCNYPKLLIVLTIAIAKATDS
                     TVFAHQANIISTNLFCVIKNRYSHVLNYLAGYLNAKHYCCMDNKEGNNFQLIRQNNVN
                     VAG"
BASE COUNT         2524 a         2723 c         3053 g         2563 t
ORIGIN      
        1 tccgtcatca ctttatcccg atgatccaaa ctggataccc cctttattca ttgaacgcag
       61 cgagcatttg tctgcgaaga atccggggac ggaccatatc atctggcagg cgtgggtggc
      121 aaaaaaagcg gggcagatag ttgggcgtat taccgcgcaa atcgatacct tgcaccgtga
      181 gcgttacggt gaagataccg gtcattacgg aatgattgat gccattgatg atccgcaggt
      241 ttttgccgcg ttgtttggcg cagcggaagc gtggttaaag tcacaaggtg caaataagat
      301 cagcgggcct ttcagcctga atatcaatca ggaaagcgga ttactgattg aaggttttga
      361 cactccgccc tgtgcgatga tgccacacgg caaaccgtgg tatgccgcgc atattgaaca
      421 actgggttat cacaaaggca ttgatttact ggcgtggtgg atgcagcgaa ccgatctcac
      481 gttctctccg gcgctaaaaa aactgatgga tcaggtgcgc aaaaaagtga ccattcgctg
      541 catcaatcgc cagcggtttg ccgaagagat gcagatcctg cgtgagattt tcaattctgg
      601 ctggcagcac aactggggat ttgtgccgtt taccgaacat gaattcgcga ccatgggcga
      661 tcaacttaaa tatctggtgc cggacgatat gatctacatc gctgagattg attctgcacc
      721 ctgcgcgttt attgttggct tgccgaacat caacgaggcg attgccgatc tgaacggatc
      781 gctctttccc ttcggctggg caaaattgct gtggcgtttg aaagtcagcg gtgtgcgaac
      841 tgcgcgagtg ccgctgatgg gcgtgcggga cgagtatcag ttcagccgca tcggcccggt
      901 aattgcgctg ttattgattg aagccttacg cgatccgttt gcccgtcgga agatcgatgc
      961 gctggagatg tcatggatcc tcgaaaccaa tacaggcatg aataacatgc tggaacgcat
     1021 tggggcggag ccgtacaagc gttaccgatt gtatgaaaag cagatttgat ggcagtcacc
     1081 cctgcttcat ctccagcggc accgccgagc gaatcaaatg gtggccttcc agctcgatat
     1141 cgtggagatt ctttttcacg gtccgcacat gatcacgtgc tgcgcgctgg gcgacgtgcg
     1201 gcagccgctg caacaccgcg ttgtagatcc gcgcatgctg gcgatcgatc tgcttttttt
     1261 gctgtggtcg atggtagaga ttatttacgc tggcaaacac tgaattaaac atcagatcgg
     1321 tcaatgattg cagcgtaaac accagcacct gattgtgaga agcctgacaa atggcgagat
     1381 ggaaagcgtg atccaactgc gcatgttcga tcagcgaaat ctctttgttg ttctcactgg
     1441 cggcgagcat tttttcataa cagcgggtta tcacaacaaa atcagcctgg gttcccagcg
     1501 ttgccgccag ccttgccgat tcgccctcca gtaatgcgcg aacgtcgagc agatcgtaca
     1561 gcgttcgcgg ctgcgtactg aacagatgga tcagcgggct ggcgtcctgc acccgattaa
     1621 gccgtgcgac acgagaatcg cgaccctgcg ccgtttcaat aatcccgcgc ccgcgcagca
     1681 cggtcagccc ttcacgcagt gcggagcgtg agaagccgag cttttcacac agtcgacgtt
     1741 ccgagggaag cggctgaccg accttcaaca cgccgtcgat aattaaccgt tcgatactct
     1801 ctgcaaccac ttcgcaaata gggcgacgtt catctttcat tcccggacct cgtgcacagg
     1861 taggaccaat ttttctgttc ttaactcgca aaacacgcac atcacgcaag tgattatgtt
     1921 acatcaattt aacattaagt taaccaaggc aaggtcacag agctggaaaa aaaatggtct
     1981 gaccggtagc taaagagata gacgaaaacg aaaagcccgc ttaataactg ttcacagaag
     2041 cagcgcgcaa aaatcagctg ccacacaaca caacaaagcg aagcctattc atgagcatct
     2101 tgtacgaaga gcgtcttgat ggcgctttac ccgatgtcga ccgcacatcg gtactgatgg
     2161 cactgcgtga gcatgtccct ggacttgaga tcctgcatac cgatgaggag atcattcctt
     2221 acgagtgtga cgggttgagc gcgtatcgca cgcgtccatt actggttgtt ttgccaaagc
     2281 agatggaaca ggtgacggcg attctggctg tctgccaccg cctgcgtgta ccggtggtga
     2341 cccgtggtgc aggcaccggg ctttctggtg gcgcgctgcc actggaaaaa ggtgtgttgt
     2401 tggtgatggc gcgctttaaa gagatcctcg acattaaccc cgttggtcgc cgcgcgcgcg
     2461 tgcagccggg cgtgcgtaat ctggcgatct cccaggccgt tgcgccgcat aatctctatt
     2521 acgcgccgga tccttcctca caaatcgcct gttcaattgg cggcaatgtg gcggaaaatg
     2581 ccggtggcgt tcactgcctg aaatatggtc tgaccgtaca taacctgctg aaaattgaag
     2641 tgcaaacgct ggacggcgag gcgctgacgc tgggatcgga tgcgctggat tcgcctggtt
     2701 ttgacctgct ggcgctgttc accggatcgg aaggtatgct cggcgtgacc accgaagtga
     2761 cggtaaaact gctgccgaag ccgcccgtgg cgcgggttct gttagccagc tttgactcgg
     2821 tagaaaaagc cggacttgcg gttggtgaca tcatcgccaa tggcattatc cccggcgggc
     2881 tggagatgat ggataacctg tcgatccgcg cagcggaaga ttttattcat gccggttatc
     2941 ccgtcgacgc cgaagcgatt ttgttatgcg agctggacgg cgtggagtct gacgtacagg
     3001 aagactgcga gcgggttaac gacatcttgt tgaaagcggg cgcgactgac gtccgtctgg
     3061 cacaggacga agcagagcgc gtacgtttct gggccggtcg caaaaatgcg ttcccggcgg
     3121 taggacgtat ctccccggat tactactgca tggatggcac catcccgcgc cgcgccctgc
     3181 ctggcgtact ggaaggcatt gcccgtttat cgcagcaata tgatttacgc gttgccaacg
     3241 tctttcatgc cggagacggc aacatgcacc cgttaatcct tttcgatgcc aacgaacccg
     3301 gtgaatttgc ccgcgcggaa gagctgggcg ggaagatcct cgaactctgc gttgaagttg
     3361 gcggcagcat cagtggggaa catggcatcg ggcgcgaaaa aatcaatcaa atgtgcgccc
     3421 agttcaacag cgatgaaatc acgaccttcc atgcggtcaa ggcggcgttt gatcccgatg
     3481 gtttgctgaa ccctgggaaa aacattccca cgctacaccg ctgtgctgaa tttggtgcca
     3541 tgcatgtgca tcacggtcat ttacctttcc ctgaactgga gcgtttctga tgctacgcga
     3601 gtgtgattac agtcaggcgc tgctggagca ggtgaatcag gcgattagcg ataaaacgcc
     3661 gctggtgatt cagggaagca atagcaaagc ctttttaggc cgccctgtca ccgggcaaac
     3721 gctggatgtg cgttgtcatc gaggcattgt taattacgac ccgaccgagc tggtgataac
     3781 cgcgcgtgcc ggaacgccgc tggtagccat tgaagcggcg ctggagagcg ccgggcaaat
     3841 gctcccctgc gagccgccgc attatggtga agaagccacc tggggcggga tggtcgcctg
     3901 cgggctggcg gggccgcgtc gcccgtggag cggttcggtc cgcgattttg tcctcggcac
     3961 gcgcatcatt accggcactg gaaaacatct gcgttttggt ggcgaagtga tgaaaaacgt
     4021 tgccggatac gatctctcac ggttaatggc cggaagctac ggttgtctgg gtgtgctcac
     4081 tgaaatctcg atgaaagtgt taccgcgacc acgcgccacc ctgagtctgc gtcgggaaat
     4141 cagcctgcaa gaagccatga atgaaatcgc ccagtggcaa cttcagccat tacccattag
     4201 tggcttatgt tacttcgaca atgcgttgtg gatccgcctt gagggcggcg aaggatcggt
     4261 aaaagcagcg cgtgaactgc tggggggcga agaggttgcg ggtcagttct ggcagcaatt
     4321 gcgtgaacaa caactgccgt tcttctcgtt accaggtacc ttatggcgca tttcattacc
     4381 cagcgatgcg ccgatgatgg atttacccgg cgagcaactg atcgactggg gcggggcgtt
     4441 acgctggctg aaatcgacag ccgatgacaa tcaaatccat cgcatcgccc gcaacgctgg
     4501 cggtcatgcg acccgcttta gtgccggaga tggtggcttt gccccgctac cggctccttt
     4561 attccgctat caccagcagc ttaaacagca gctcgaccct tgcggcgtgt ttaaccccgg
     4621 tcgcatgtac gcggaacttt gaggagcagg ctatgcaaac ccgattaaca gaagagatgc
     4681 ggcagaacgc gcgcgcgctg gaagccgaca gcatcctgcg cgcctgtgtt cactgcggat
     4741 tttgtaccgc aacctgccca acctatcagc ttctgggcga tgaactggac gggccgcgcg
     4801 ggcgcatcta tctgattaaa caggtactgg aaggcaacga agtcacgctt aaaacacagg
     4861 agcatctcga tcgctgtctc acttgccgca attgtgaaac cacctgtcct tctggtgtgc
     4921 gctatcacaa tttgctggat atcgggcgtg atattgtcga gcagaaagtg aaacgcccac
     4981 tgccggagcg aatgctgcgc gaaggattgc gccaggtagt gccgcgtccg gcggtcttcc
     5041 gtgcgctgac gcaggtaggg ctggtgctgc gaccgttttt accggaacag gtcagagcaa
     5101 aactgcctgc tgaaacggtg aaagctaaac cgcgtccgcc gctgcgccat aagcgtcggg
     5161 ttttaatgct ggaaggctgc gcccagccta cgctttcgcc caacaccaac gcggcaactg
     5221 cgcgagtgct ggatcgtctg gggatcagcg tgacgccagc taacgaagca ggctgttgtg
     5281 gcgcggtgga ctatcatctt aatgcgcaag agaaagggct ggcgcgggcg cgcaataata
     5341 ttgatgcctg gtggcccgcg attgaagcag gtgtcgaggc aattttgcaa accgccagcg
     5401 gttgcggcgc gttcgttaaa gagtatgggc agatgctgaa aaacgatgcg ttgtatgccg
     5461 ataaagcgcg tctggtcagt gaactggcgg tcgatttagt cgaacttctg cgcgaggaac
     5521 cgctggaaaa actggcaatt cgcggcgata aaaaactggc cttccactgc ccgtgtaccc
     5581 tacaacatgc gcaaaagctg aacggcgaag tggaaaaagt gttacttcgt cttggattta
     5641 ccttaacgga cgttcccgac agccatctgt gctgcggttc agcgggaaca tatgcgttaa
     5701 cgcatcccga tctggcacgc cagctgcggg ataacaaaat gaatgcgctg gaaagcggca
     5761 aaccggaaat gatcgtcacc gccaacattg gttgccagac gcatctggcg agcgccgatc
     5821 gtacctctgt gcgtcactgg attgaaattg tagaacaagc ccttgaaaag gaataacaaa
     5881 atgaaaacta aagtcattct tagccagcaa atggcgagtg caattattgc cgcaggtcag
     5941 gaagaggcgc agaaaaataa ctggtctgtt tccattgctg ttgccgatga cggcggtcat
     6001 ctgctggcgt taagtcgcat ggacgattgc gcgccgattg cggcttacat ctcccaggag
     6061 aaagcgcgta ccgccgcgct ggggcgtcgt gaaactaagg gctatgaaga gatggtgaac
     6121 aacggacgta ccgcgttcgt gactgcgccg ttactgacgt cgctggaagg tggcgtaccg
     6181 gttgttgtgg atgggcaaat tattggtgcc gtgggcgttt ctggtttaac cggagcacag
     6241 gatgcgcagg tcgcgaaagc ggcagcagcg gtgttggcga aataagcgaa aacgaggaga
     6301 taaacaatga gtcaaaccat aacccagggc cgtttacgca ttaacgccaa ttttaaacgt
     6361 tttgtggatg aagaagtttt accgggaaca gggttggaca ctgcggcgtt ctggcgcaat
     6421 tttgatgaga tcgttcacga tctggcacca gaaaatcgtc agttgctggc agaacgcgat
     6481 cgcattcagg cggcgcttga tgagtggcat cgcagcaatc cggggccggt aaaagataaa
     6541 gcggcctata aatctttcct gcgtgaactg ggctacctgg tgccgcaacc ggagcgcgtg
     6601 acggtggaaa ccacgggcat tgacagcgaa atcaccagcc aggcggggcc acagctggtg
     6661 gttccggcaa tgaacgcccg ctacgcgctg aacgcggcga acgctcgctg gggctcactg
     6721 tacgatgcgt tatacggcag cgacatcatc ccgcaggaag gggcgatggt cagcggctac
     6781 gatccgcaac gcggtgagca ggttatcgcc tgggttcggc gtttcctcga tgaatcacta
     6841 ccgctggaaa acggcagcta tcaggatgtg gtggcgttta aggtggtcga taaacaatta
     6901 cgcatccagt tgaaaaatgg taaagaaacc acgttacgta ccccggcgca gtttgtcggt
     6961 taccgtggcg atgccgctgc gccgtcctgc attttgctga aaaataacgg cctgcatatt
     7021 gagctacaaa tcgatgccaa cgggcggatt ggcaaagacg attcggcgca catcaacgat
     7081 gttatcgtcg aagcggccat cagtaccatt ctcgactgcg aagattcggt tgcggcggtt
     7141 gatgcggaag ataaaatcct gctgtaccgc aatctgctgg gtctgatgca ggggactctg
     7201 caagagaaaa tggagaaaaa cggtcggcaa atcgtgcgta aactgaatga cgatcgtcag
     7261 tacaccgctg ccgacggctc tgaaatttct ctgcacgggc gttcgctgct gtttatccgc
     7321 aacgtgggtc atttgatgac cattcctgtg atttgggaca gcgaaggcaa tgaaatcccg
     7381 gaaggcattc ttgatggcgt catgactggc gcgattgccc tctatgattt aaaagtgcag
     7441 aaaaactcgc gcactggcag cgtctatatt gtgaaaccga aaatgcacgg tccgcatgaa
     7501 gtggcgttcg ccaacaaact gtttacccgc attgagacaa tgctcggtat ggcaccgaat
     7561 accctgaaaa tgggcattat ggatgaagaa cgtcggacct cgctgaactt gcgtagctgt
     7621 atcgctcagg cgcgtaaccg cgtggcgttc atcaataccg gtttcctcga ccgtaccggc
     7681 gatgaaatgc attcggtgat ggaagctggc ccgatgctgc gtaaaaacca gatgaaatcg
     7741 acgccgtgga tcaaagccta cgaacgtaat aacgtgcttt ccggtctgtt ctgtgggctg
     7801 cgcggtaaag cgcaaatcgg taaaggcatg tgggcaatgc cggacctgat ggcagacatg
     7861 tacagccaga agggcgacca gctgcgtgcc ggggcaaaca cggcctgggt tccgtcgcca
     7921 actgccgcta cgctccatgc gctgcactac caccaaacca acgtacagag cgtgcaagcc
     7981 aacattgccc agaccgagtt caatgctgaa tttgaaccgc tgctggacga tctgctgact
     8041 attccggttg ctgaaaacgc taattggtcg gcgcaagaga tccaacaaga gctggataac
     8101 aacgtgcagg ggattctggg gtacgtggtg cgttgggtgg agcaggggat tggttgttca
     8161 aaagtgccgg atattcacaa cgtggcgttg atggaagacc gcgcaacgct gcgtatttcc
     8221 agccagcata tcgccaactg gttacgtcac ggtattctga ccaaagatca ggtgcaggcg
     8281 tcgctggaga atatggcgaa agtggttgat cagcaaaacg ctggcgatcc ggcttatcgt
     8341 ccaatggtgg agaatttcgc taactcgtgt gcttttaaag ctgcctgcga tttgatcttc
     8401 ctcggagtga aacagccaaa cggctatacc gaaccgttat tacacgcatg gcgtttacgc
     8461 gaaaaagaaa atcattaata gcgtcaaaaa aagccccggc aagatttatt gtcggggcta
     8521 tgatagtaac tgcggtagta catttatccc ggaaataacc ggagcgatta tcaggggtta
     8581 acaatattaa atacccgtag cgcatcacta gcgggattaa aacaacaata tggacatctt
     8641 aacgatactc tgcgcctgcg tgtgcggaac agaaaccctt acccgatata cttgttatgc
     8701 cagaggtagc aattgcgtta gcggcgtact gttgctgtaa agtataacgc ctatatttta
     8761 cagttcagtc ctcttttgct tattacaacc ttaagtatat caagcatata aagataataa
     8821 gagactgaac aatatggtta cctggaccca aatgtatatg ccgatgggag gactggggct
     8881 atccgctctg gtcgccctga tcccaataat attcttcttc gttgcactcg cggtattacg
     8941 tctgaaagga catgtcgctg gagcaataac ccttatatta tctattctga ttgcgatatt
     9001 cgcctttaaa atgccgattg atatggcatt tgctgctgcg ggctatggct ttatttatgg
     9061 tttatggcct attgcgtgga ttattgtcgc ggcggtattc ctgtataaat taaccgttgc
     9121 cagcggccag ttcgatatta tccgcagctc ggttatctcc atcaccgacg atcagcgttt
     9181 gcaggtgtta ctgattggtt tctcctttgg tgcgctgctg gaaggggcgg ctggctttgg
     9241 cgcaccggtg gcgattaccg gtgctctact ggtgggcctg ggcttcaaac cgctatacgc
     9301 ggcggggctg tgtctgattg ccaacactgc gccggtggcg tttggtgcgt tgggtgtgcc
     9361 aattctggtc gccggtcagg taacgggaat cgatccgttc gacattggcg caatggcggg
     9421 acgtcagtta ccgttcctgt cggttcttgt gccgttctgg ctggtagcaa tgatggacgg
     9481 ctggaaaggg gtgaaagaga cgtggccagc ggcgctggtt gcagggggaa gcttcgctgt
     9541 cactcagttc tttacctcta actatattgg tccggaactg ccggatatta cttcggcgct
     9601 ggtgagtatc gtctcacttt ctttatttct taaagtctgg cggccaaaaa ataccgaaac
     9661 ggcaatcagt atgggacaat ccgcaggtgc gatggtggtg aataaaccgt cttctggcgg
     9721 tcccgtgcct tcagaatatc gtctggggca aatcattcgt gcgtggtcac cgtttttaat
     9781 cttaacggtg ttggtcacca tctggaccat gaagccgttt aaagcgttat ttgctccggg
     9841 cggcgcgttt tattcgctgg tgattaattt ccagatccct catttgcatc aacaagtgtt
     9901 gaaagcggcc cccattgtcg cccagccgac gccaatggac gcggtgttta aattcgaccc
     9961 cctctctgct ggtggcaccg ctatttttat tgcggcgatt atctcgatct tcatcctcgg
    10021 cgtggggatc aagaaaggta ttggtgtctt tgccgaaacg ctaattagtc tgaagtggcc
    10081 gatactgtcg attggcatgg tgctggcatt tgccttcgtg accaactatt ccggcatgtc
    10141 caccacgctg gcgctggtac tggctggtac aggcgtaatg ttcccgtttt tctcaccgtt
    10201 tctcggctgg ctgggcgtat tccttaccgg ctcggacacc tcctctaacg ccctgtttgg
    10261 ttcactgcaa tcgaccacgg cgcagcaaat caacgtctct gacaccctgc tggtggcagc
    10321 aaataccagc ggcggcgtaa ctggcaagat gatctccccg caatctatcg ccgtggcctg
    10381 cgccgcgacg ggcatggtgg gccgagaatc tgaactgttc cgctacaccg tgaagcacag
    10441 tctgattttt gccagcgtta tcggcattat cacgctgttg caggcgtatg tgtttaccgg
    10501 gatgttagtt tcgtaagtca ataatgccgg atgatcgtta atatcatccg gctacattta
    10561 cattattctg tcttattaat tgaaaattat ttccctcttt attatccatg cagcaataat
    10621 gttttgcgtt cagataacct gctaaataat taagaacgtg agaatatcta ttttttatca
    10681 cgcaaaacaa gtttgtacta attatgttcg cttgatgagc aaacaccgtg ctatctgttg
    10741 cttttgcaat cgcaatcgtt aaaactatca acaacttggg atagttacat tgtgaataat
    10801 tgttactttt ttgtttgttg aatcttttta taaatacccc gtctgacatc gaatagggga
    10861 tat
//
LOCUS       DAZFLD010000158        10848 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.159, whole genome shotgun sequence.
ACCESSION   DAZFLD010000158 DAZFLD010000000
VERSION     DAZFLD010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10848)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10848)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10848
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.159"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            261..665
                     /gene="sdhC"
                     /locus_tag="ACHKBL_003587"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
     CDS             261..665
                     /gene="sdhC"
                     /locus_tag="ACHKBL_003587"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0045273 - respiratory chain complex II
                     (succinate dehydrogenase) [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HFO5820404.1"
                     /translation="MWALFMIRNVKKQRPVNLDLQTIRFPITAIASILHRVSGVITFV
                     AVGILLWLLGTSLSSPEGFEQASAIMGSFFVKFIMWGILTALAYHVVVGIRHMMMDFG
                     YLEETFEAGKRSAKISFVITVVLSLLAGVLVW"
     gene            659..1006
                     /gene="sdhD"
                     /locus_tag="ACHKBL_003588"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
     CDS             659..1006
                     /gene="sdhD"
                     /locus_tag="ACHKBL_003588"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase membrane anchor subunit"
                     /protein_id="HFO5820405.1"
                     /translation="MVSNASALGRNGVHDFILVRATAIVLTLYIIYMVGFFATSGELT
                     YEVWIGFFASAFTKVFTLLALFSILIHAWIGMWQVLTDYVKPLALRLMLQLVIVVALV
                     VYVIYGFVVVWGV"
     gene            1006..2772
                     /gene="sdhA"
                     /locus_tag="ACHKBL_003589"
     CDS             1006..2772
                     /gene="sdhA"
                     /locus_tag="ACHKBL_003589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HFO5820406.1"
                     /translation="MKLPVREFDAVVIGAGGAGMRAALQISQSGQTCALLSKVFPTRS
                     HTVSAQGGITVALGNTHEDNWEWHMYDTVKGSDYIGDQDAIEYMCKTGPEAILELEHM
                     GLPFSRLDDGRIYQRPFGGQSKNFGGEQAARTAAAADRTGHALLHTLYQQNLKNHTTI
                     FSEWYALDLVKNQDGAVVGCTALCIETGEVVYFKARATVLATGGAGRIYQSTTNAHIN
                     TGDGVGMAIRAGVPVQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLLNKHGERFMERY
                     APNAKDLAGRDVVARSIMIEIREGRGCDGPWGPHAKLKLDHLGKEVLESRLPGILELS
                     RTFAHVDPVKEPIPVIPTCHYMMGGIPTKVTGQALTVNEKGEDVVVPGLFAVGEIACV
                     SVHGANRLGGNSLLDLVVFGRAAGLHLQESIAEQGALRDASASDVEASLDRLNRWNNN
                     RNGEDPVAIRKALQECMQHNFSVFREGDAMAKGLEQLKVIRERLKNARLDDTSSEFNT
                     QRVECLELDNLMETAYATAVSANFRTESRGAHSRFDFPDRDDENWLCHSLYLPESESM
                     TRRSVNMEPKLRPAFPPKIRTY"
     gene            2788..3504
                     /gene="sdhB"
                     /locus_tag="ACHKBL_003590"
     CDS             2788..3504
                     /gene="sdhB"
                     /locus_tag="ACHKBL_003590"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit
                     SdhB"
                     /protein_id="HFO5820407.1"
                     /translation="MRLEFSIYRYNPDVDDAPRMQDYTLEAEEGRDMMLLDALIQLKE
                     KDPSLSFRRSCREGVCGSDGLNMNGKNGLACITPISALNQPGKKIVIRPLPGLPVIRD
                     LVVDMGQFYAQYEKIKPYLLNNGQNPPAREHLQMPEQREKLDGLYECILCACCSTSCP
                     SFWWNPDKFIGPAGLLAAYRFLIDSRDTETDSRLDGLSDAFSVFRCHSIMNCVSVCPK
                     GLNPTRAIGHIKSMLLQRNA"
     gene            3805..6606
                     /gene="sucA"
                     /locus_tag="ACHKBL_003591"
     CDS             3805..6606
                     /gene="sucA"
                     /locus_tag="ACHKBL_003591"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HFO5820408.1"
                     /translation="MQNSALKAWLDSSYLSGANQSWIEQLYEDFLTDPDSVDANWRST
                     FQQLPGTGVKPDQFHSQTREYFRRLAKDASRYSSTISDPDTNVKQVKVLQLINAYRFR
                     GHQHANLDPLGLWQQDKVADLDPSFHDLTEADFQETFNVGSFASGKETMKLGELLEAL
                     KQTYCGPIGAEYMHITSTEEKRWIQQRIESGRATFNSEEKKRFLSELTAAEGLERYLG
                     AKFPGAKRFSLEGGDALIPMLKEMIRHAGNSGTREVVLGMAHRGRLNVLVNVLGKKPQ
                     DLFDEFAGKHKEHLGTGDVKYHMGFSSDFQTDGGLVHLALAFNPSHLEIVSPVVIGSV
                     RARLDRLDEPSSNKVLPITIHGDAAVTGQGVVQETLNMSKARGYEVGGTVRIVINNQV
                     GFTTSNPLDARSTPYCTDIGKMVQAPIFHVNADDPEAVAFVTRLALDFRNTFKRDVFI
                     DLVCYRRHGHNEADEPSATQPLMYQKIKKHPTPRKIYADKLEQEKVATLEDATEMVNL
                     YRDALDAGDCVVAEWRPMNMHSFTWSPYLNHEWDEEYPNKVEMKRLQELAKRISTVPE
                     AVEMQSRVAKIYGDRQAMAAGEKLFDWGGAENLAYATLVDEGIPVRLSGEDSGRGTFF
                     HRHAVIHNQSNGSTYTPLQHIHNGQGAFRVWDSVLSEEAVLAFEYGYATAEPRTLTIW
                     EAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARLERYLQL
                     CAEQNMQVCVPSTPAQVYHMLRRQALRGMRRPLVVMSPKSLLRHPLAVSSLEELANGT
                     FLPAIGEIDELDPKGVKRVVMCSGKVYYDLLEQRRKNNQHDVAIVRIEQLYPFPHKAM
                     QEVLQQFAHVKDFVWCQEEPLNQGAWYCSQHHFREVIPFGASLRYAGRPASASPAVGY
                     MSVHQKQQQDLVNDALNVE"
     gene            6621..7838
                     /gene="odhB"
                     /locus_tag="ACHKBL_003592"
     CDS             6621..7838
                     /gene="odhB"
                     /locus_tag="ACHKBL_003592"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HFO5820409.1"
                     /translation="MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDK
                     VVLEVPASADGILDAVLEDEGTTVTSRQILGRLREGNSAGKETSAKSEEKASTPAQRQ
                     QASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKAPVKESAP
                     AAPAPAAQPALAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRK
                     QYGEAFEKRHGIRLGFMSFYVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTP
                     RGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMS
                     TPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLVTIK
                     ELLEDPTRLLLDV"
     gene            7932..9098
                     /gene="sucC"
                     /locus_tag="ACHKBL_003593"
     CDS             7932..9098
                     /gene="sucC"
                     /locus_tag="ACHKBL_003593"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HFO5820410.1"
                     /translation="MNLHEYQAKQLFARYGLPAPVGYACTTPREAEEAASKIGAGPWV
                     VKCQVHAGGRGKAGGVKVVNSKEDIRAFAENWLGKRLVTYQTDANGQPVNQILVEAAT
                     DIAKELYLGAVVDRSSRRVVFMASTEGGVEIEKVAEETPHLIHKVALDPLTGPMPYQG
                     RELAFKLGLEGKLVQQFTKIFMGLATIFLERDLALIEINPLVITKQGDLICLDGKLGA
                     DGNALFRQPDLREMRDQSQEDPREAQAAQWELNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLHGGEPANFLDVGGGATKERVTEAFKIILSDDKVKAVLVNIFGGIVRCDLIADGIIG
                     AVAEVGVNVPVVVRLEGNNAELGAKKLADSGLNIIAAKGLTDAAQQVVAAVEGK"
     gene            9098..9967
                     /gene="sucD"
                     /locus_tag="ACHKBL_003594"
     CDS             9098..9967
                     /gene="sucD"
                     /locus_tag="ACHKBL_003594"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HFO5820411.1"
                     /translation="MSILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKG
                     GTTHLGLPVFNTVREAVAATGATASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPT
                     LDMLTVKVKLDEAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIVSRSGTLTYE
                     AVKQTTDYGFGQSTCVGIGGDPIPGSNFIDILEMFEKDPQTEAIVMIGEIGGSAEEEA
                     AAYIKEHVTKPVVGYIAGVTAPKGKRMGHAGAIIAGGKGTADEKFAALEAAGVKTVRS
                     LADIGEALKTVLK"
     gene            complement(10071..10793)
                     /gene="mngR"
                     /locus_tag="ACHKBL_003595"
     CDS             complement(10071..10793)
                     /gene="mngR"
                     /locus_tag="ACHKBL_003595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-D-glycerate transport/metabolism system
                     transcriptional repressor MngR"
                     /protein_id="HFO5820412.1"
                     /translation="MGRKPLYRQIADRIREQIARGELKPGDALPTESALQTEFGVSRV
                     TVRQALRQLVEQQILESIQGSGTYVKEERVNYDIFQLTSFDEKLTDRHVDTHSEVLIF
                     EVIPADDFLQQQLQITAQDRVWHVKRVRYRKQKPMALEETWMPLALFPDLTWQVMENS
                     KYHFIEEVKKMVIDRSEQEIIPLMPTEEMSRLLNISQTKPILEKVSRGYLVDGRVFEY
                     SRNAFNTDDYKFTLIAQRKSSR"
BASE COUNT         2560 a         2813 c         2992 g         2483 t
ORIGIN      
        1 aaactatatg taggttaatt gtaatgattt tgtgaacggc ctatactgcc gccaggtctc
       61 cggaacaccc tgcaatcccg agccacccag cgttgtaacg tgtcgttttc gcatctggaa
      121 gcagtgtttt gcatgacgcg cagttataga aaggacgctg tctgacccgc aagcagaccg
      181 gaggaaggaa atcccgacgt ctccaggtaa cagaaagtta acctctgtgc ccgtagtccc
      241 cagggaataa taagaacagc atgtgggcgt tattcatgat aagaaatgtg aaaaaacaaa
      301 gacctgttaa tctggaccta cagaccatcc ggttccccat cacggcgata gcgtccattc
      361 tccatcgcgt ttccggtgtg atcacctttg ttgcagtggg catcctgctg tggcttctgg
      421 gtaccagcct ctcttcccct gaaggtttcg agcaagcttc cgcgattatg ggcagcttct
      481 tcgtcaaatt tatcatgtgg ggcatcctta ccgctctggc gtatcacgtc gtcgtaggta
      541 ttcgccacat gatgatggat tttggctatc tggaagaaac attcgaagcg ggtaaacgct
      601 ccgccaaaat atcctttgtt attactgtcg tgctttcact tctcgcagga gtcctcgtat
      661 ggtaagcaac gcctccgcat taggacgcaa tggcgtacat gatttcatcc tcgttcgcgc
      721 taccgctatc gtcctgacgc tctacatcat ttatatggtc ggttttttcg ctaccagtgg
      781 cgagctgaca tatgaagtct ggatcggttt cttcgcctct gcgttcacca aagtgttcac
      841 cctgctggcg ctgttttcta tcttgatcca tgcctggatc ggcatgtggc aggtgttgac
      901 cgactacgtt aaaccgctgg ccttgcgcct gatgctgcaa ctggtgattg tcgttgctct
      961 ggtggtttac gtgatttatg gattcgttgt ggtgtggggt gtgtgatgaa attgccagtc
     1021 agagaatttg atgcagttgt aattggtgcc ggtggcgcag gtatgcgcgc ggcgctgcaa
     1081 atttcccaga gcggtcagac ctgtgcgctg ctctctaaag tcttcccgac ccgttcccat
     1141 accgtttctg cgcaaggcgg cattaccgtt gcgctgggta atacccatga agataactgg
     1201 gaatggcata tgtacgacac cgtaaaaggt tcggactata tcggtgacca ggacgcgatt
     1261 gaatatatgt gtaaaaccgg gccggaagcg attctggaac tggaacatat gggcctgccg
     1321 ttctcgcgtc ttgatgatgg tcgtatctat caacgtccgt ttggcggtca gtcgaaaaac
     1381 ttcggcggcg agcaggcggc acgtactgcg gcggcagctg accgtaccgg tcacgcactg
     1441 ttgcacaccc tttatcaaca gaacctgaaa aaccacacca ccattttctc cgagtggtat
     1501 gcgctggatc tggtgaaaaa ccaggatggc gcggtggtcg gttgtaccgc actgtgcatc
     1561 gaaactggtg aagtggttta cttcaaagct cgcgctaccg tgctggcgac tggcggggca
     1621 gggcgtattt atcagtccac taccaacgcc cacattaaca ctggcgacgg cgtcggcatg
     1681 gctatccgtg caggcgtacc agtgcaggat atggaaatgt ggcagttcca cccgactggt
     1741 attgccggtg cgggcgtact ggtcaccgaa gggtgccgtg gtgaaggcgg ttatctgctg
     1801 aacaaacatg gcgaacgctt tatggagcgt tatgcaccga acgccaaaga cctggcgggc
     1861 cgtgacgtgg tggcgcgttc catcatgatt gaaatccgtg aaggtcgcgg ctgtgatggt
     1921 ccgtgggggc cacacgcgaa actgaaactt gaccatctgg gtaaagaagt tcttgaatcc
     1981 cgtctgccgg gtatccttga actctcccgc accttcgctc acgttgatcc ggtgaaagag
     2041 ccgattccgg ttatcccaac ctgtcactac atgatgggcg gtattccgac caaagtgacc
     2101 ggtcaggcgc tgacggtgaa cgagaaaggc gaagatgtgg ttgttccggg gctatttgcc
     2161 gttggtgaaa tcgcttgtgt atcggtacat ggcgctaacc gtctgggcgg caactcgctg
     2221 ctggacctgg tcgtatttgg tcgtgcggca ggtctgcatc tgcaagagtc tatcgccgag
     2281 cagggcgcac tgcgcgatgc cagcgcgtct gatgtagaag cgtctctgga tcgcctgaac
     2341 cgctggaaca ataaccgtaa cggtgaagat ccggtggcga tccgtaaagc actgcaagaa
     2401 tgtatgcagc ataacttctc ggtcttccgt gaaggtgatg cgatggcgaa agggcttgag
     2461 cagttgaaag ttatccgcga gcgtctgaaa aatgcccgtc tggatgacac ttcaagtgag
     2521 ttcaataccc agcgcgttga gtgcctggaa ctggataacc tgatggaaac ggcgtatgca
     2581 acggctgttt ctgccaactt ccgtaccgaa agccgtggcg cacatagccg cttcgacttc
     2641 ccggatcgtg atgatgaaaa ctggctgtgc cactccctgt atctgcctga gtcggaatcc
     2701 atgacgcgcc gaagcgtcaa catggaaccg aaactgcgcc cggcattccc gccgaagatt
     2761 cgtacttact aatgcggaga caggaaaatg agactcgagt tttcaattta tcgctataac
     2821 ccggatgttg atgatgctcc gcgtatgcag gattacaccc tggaagcgga agaaggtcgt
     2881 gacatgatgc tgctggatgc gcttatccag ctgaaagaga aagatcccag cctgtcgttc
     2941 cgtcgctcct gccgtgaagg tgtgtgtggt tccgacggtc tgaacatgaa cggcaagaat
     3001 ggtctggcct gtattacccc gatttcggca ctcaaccagc cgggcaagaa gattgtgatt
     3061 cgcccgctgc caggtttacc ggtgatccgc gatttggtgg tagacatggg acaattctat
     3121 gcgcaatatg agaaaattaa gccttacctg ttgaataatg gacaaaatcc gccagctcgc
     3181 gagcatttac agatgccaga gcagcgcgaa aaactcgacg ggttgtatga atgtattctc
     3241 tgcgcatgtt gttcaacctc ttgtccgtct ttctggtgga atcccgataa gtttatcggc
     3301 ccggcaggct tgttagcggc atatcgtttc ctgatcgata gccgtgatac cgagactgac
     3361 agccgcctcg acggtttgag cgatgcattc agtgtattcc gctgtcacag catcatgaac
     3421 tgcgtcagtg tatgtccgaa ggggctgaac ccgacgcgcg ccatcggcca tatcaagtcg
     3481 atgttgttgc aacgtaatgc gtaaaccgta ggcctgagaa gacgcgcaag cgtcgcatca
     3541 ggcaaccagt gccggatgcg gcgtgaacgc cttatccggc ctacaagtca ttacccgtag
     3601 gcctgataag cgcagcgcat caggcgtaac aaagaaatgc aggaaatctt taaaaactgc
     3661 ccctgacact aagacagttt ttaaaggttc cttcgcgagc cactacgtag acaagagctc
     3721 gcaagtgaac cccggcacgc acatcactgt gcgtggtagt atccacggcg aagtaagcat
     3781 aaaaaagatg cttaagggat cacgatgcag aacagcgctt tgaaagcctg gttggactct
     3841 tcttacctct ctggcgcaaa ccagagctgg atagaacagc tctatgaaga cttcttaacc
     3901 gatcctgact cggttgacgc taactggcgt tcgacgttcc agcagttacc tggtacggga
     3961 gtcaaaccgg atcaattcca ctctcaaacg cgtgaatatt tccgccgcct ggcgaaagac
     4021 gcttcacgtt actcttcaac gatctccgac cctgacacca atgtgaagca ggttaaagtc
     4081 ctgcagctca ttaacgcata ccgcttccgt ggtcaccagc atgcgaatct cgatccgctg
     4141 ggactgtggc agcaagataa agtggccgat ctggatccgt ctttccacga tctgaccgaa
     4201 gcagacttcc aggagacctt caacgtcggt tcatttgcca gcggcaaaga aaccatgaaa
     4261 ctcggcgagc tgctggaagc cctcaagcaa acctactgcg gcccgattgg tgccgagtat
     4321 atgcacatta ccagcactga agaaaaacgc tggatccaac agcgtatcga gtctggtcgc
     4381 gcgactttca atagcgaaga gaaaaaacgc ttcttaagcg aactgaccgc cgctgaaggc
     4441 cttgaacgtt acctcggcgc aaaattccct ggcgcaaaac gcttctcgct ggaaggtggt
     4501 gacgcgttaa tcccgatgct taaagagatg atccgccacg ctggcaacag cggcacccgc
     4561 gaagtggttc tcgggatggc gcaccgtggt cgtctgaacg tgctggtgaa cgtgctgggt
     4621 aaaaaaccgc aagacttgtt cgacgagttt gccggtaaac ataaagaaca cctcggcacg
     4681 ggcgacgtga aataccacat gggcttctcg tctgacttcc agaccgatgg cggcctggtg
     4741 cacctggcgc tggcgtttaa cccgtctcac cttgagattg tcagcccggt cgttatcggt
     4801 tctgttcgtg cccgtctgga cagacttgat gagccgagca gcaacaaagt gctgccaatc
     4861 accattcatg gtgacgccgc agtgaccggg cagggcgtgg ttcaggaaac cctgaacatg
     4921 tcgaaagcgc gtggttatga agttggcggt acggtacgta tcgttatcaa caaccaggtt
     4981 ggcttcacca cctctaaccc gctggatgcc cgttcgacgc cgtactgtac tgatatcggc
     5041 aagatggtac aggcaccgat tttccacgtt aatgcggatg atccggaagc cgttgccttt
     5101 gtgacccgtc tggcgctcga tttccgtaac acctttaaac gtgatgtctt catcgatctg
     5161 gtgtgctacc gccgtcacgg ccacaacgaa gccgacgagc cgagcgcaac ccagccgctg
     5221 atgtatcaga aaatcaaaaa acatccgacg ccgcgcaaaa tctatgctga caagctggag
     5281 caggaaaaag tcgcgacgct ggaagatgcc accgagatgg ttaacctgta ccgcgatgcg
     5341 ctggatgctg gcgattgcgt tgtagcagag tggcgtccga tgaacatgca ctctttcacc
     5401 tggtcgccgt acctcaacca cgaatgggac gaagagtacc cgaacaaagt tgagatgaag
     5461 cgcctgcagg aactggctaa acgcatcagc acggtgccgg aagcagttga aatgcagtct
     5521 cgcgttgcca agatttatgg cgatcgccag gcaatggcag ccggtgagaa actgttcgac
     5581 tggggcggtg cggaaaacct cgcttacgcc acgctggttg atgaaggcat tccggttcgc
     5641 ctgtcgggtg aagactccgg tcgcggtacc ttcttccacc gccacgcggt gatccacaac
     5701 cagtctaacg gttccactta cacgccgctg caacacatcc ataacggtca gggcgcgttc
     5761 cgtgtctggg actccgtact gtcagaagaa gccgtgctgg cgtttgaata cggttatgcc
     5821 accgcagaac cacgcactct gaccatctgg gaagcgcagt tcggtgactt cgccaacggt
     5881 gcgcaggtgg ttatcgacca gttcatctcc tctggcgaac agaaatgggg ccggatgtgt
     5941 ggcctggtga tgttgctgcc gcacggttac gaagggcagg ggccggagca ctcctccgcg
     6001 cgtctggaac gttatctgca actttgcgct gagcaaaaca tgcaggtttg cgtaccgtct
     6061 accccggcac aggtttacca catgctgcgt cgtcaggcgt tgcgcgggat gcgtcgtcca
     6121 ctggtcgtga tgtcgccgaa atccctgctg cgtcatccgc tggcggtatc cagcctcgaa
     6181 gaactggcga acggcacctt cctgccagcc atcggtgaaa tcgacgagct tgatccgaag
     6241 ggcgtgaagc gcgtagtgat gtgttctggt aaggtttatt acgacctgct ggaacaacgt
     6301 cgtaagaaca atcaacacga tgtcgccatt gtgcgtatcg agcaactcta cccgttcccg
     6361 cataaagcga tgcaggaagt gttgcagcag tttgctcacg tcaaggattt tgtctggtgc
     6421 caggaagagc cgctcaacca gggcgcatgg tactgcagcc agcatcattt ccgtgaagtg
     6481 attccgtttg gggcttctct gcgttatgca ggccgcccgg cctccgcctc tccggcggta
     6541 gggtatatgt ccgttcacca gaaacagcaa caagatctgg ttaatgacgc gctgaacgtc
     6601 gaataaataa aggatacaca atgagtagcg tagatattct ggtccctgac ctgcctgaat
     6661 ccgtagccga tgccaccgtc gcaacctggc ataaaaaacc cggcgacgca gtcgtacgtg
     6721 atgaagtgct ggtagaaatc gaaactgaca aagtggtact ggaagtaccg gcatcagcag
     6781 acggcattct ggatgcggtt ctggaagatg aaggtacaac ggtaacgtct cgtcagatcc
     6841 ttggtcgcct gcgtgaaggc aacagcgcgg gtaaagaaac cagcgccaaa tctgaagaga
     6901 aagcgtccac tccggcgcaa cgccagcagg cgtctctgga agagcaaaac aacgatgcgt
     6961 taagcccggc gatccgtcgc ctgctggctg aacataacct cgacgccagc gccattaaag
     7021 gcaccggtgt gggtggtcgt ctgacccgtg aagatgtgga aaaacatctg gcgaaagccc
     7081 cggtgaaaga gtctgctccg gcagcgcctg ctccggcggc gcaaccggcc ctggctgcac
     7141 gtagcgaaaa acgtgtgccg atgactcgcc tgcgtaagcg tgtggcagag cgtctgctgg
     7201 aagcgaaaaa ctccaccgcc atgctgacca cgttcaacga agtcaacatg aagccgatta
     7261 tggatctgcg taagcagtac ggtgaagcgt ttgaaaaacg ccacggcatc cgtctgggct
     7321 ttatgtcctt ctacgtgaaa gcggtggttg aagccctgaa acgttacccg gaagtgaatg
     7381 cgtctatcga cggtgatgat gtggtctacc acaactattt cgacgtcagt atggcggttt
     7441 ctacgccgcg cggcctggtg acgccggttc tgcgtgatgt cgataccctc ggcatggcag
     7501 acatcgagaa gaaaatcaaa gagctggctg ttaaaggccg tgacggcaag ctgacggttg
     7561 aagatctgac tggtggtaac ttcaccatca ccaacggtgg tgtgttcggt tccctgatgt
     7621 caacgcctat catcaaccca ccgcagagcg caattctggg tatgcacgct atcaaagatc
     7681 gtccgatggc ggtgaatggt caggttgaga tcctgccgat gatgtacctg gcgctgtcct
     7741 acgatcaccg tctgatcgat ggtcgcgaat ccgtgggctt cctggtaacg atcaaagagt
     7801 tgctggaaga tccgacgcgt ctgctgctgg acgtgtagta gtttaagctt cacctgcact
     7861 gtagaccgac gtggataggg tgtcggtcta cggtttaaaa gataacgatt actgaaggat
     7921 ggacagaaca catgaactta catgaatatc aggcaaaaca actttttgcc cgctatggct
     7981 taccagcacc ggtgggttat gcctgtacta ctccgcgcga agcagaagaa gccgcttcaa
     8041 aaatcggtgc cggtccgtgg gtagtgaaat gtcaggttca cgctggtggc cgcggtaaag
     8101 cgggcggtgt gaaagttgta aacagcaaag aagacatccg tgcttttgca gaaaactggc
     8161 tgggcaagcg tctggtaacg tatcaaacag atgccaatgg ccaaccggtt aaccagattc
     8221 tggttgaagc agcgaccgat atcgctaaag agctgtatct cggtgccgtt gttgaccgta
     8281 gttcccgtcg tgtggtcttt atggcctcca ccgaaggcgg cgtggaaatc gaaaaagtgg
     8341 cggaagaaac tccgcacctg atccacaaag ttgcgcttga tccgctgact ggcccgatgc
     8401 cgtatcaggg acgcgagctg gcgttcaaac tgggtctgga aggtaaactg gttcagcagt
     8461 tcaccaaaat cttcatgggc ctggcgacca ttttcctgga gcgcgacctg gcgctgatcg
     8521 aaatcaaccc gctggtcatt accaaacagg gcgatctgat ttgcctcgac ggcaaactgg
     8581 gcgctgatgg caacgcactg ttccgccagc ctgatctgcg cgaaatgcgt gaccagtcgc
     8641 aggaagatcc gcgtgaagca caggctgcac agtgggaact gaactacgtt gcgctggacg
     8701 gtaacatcgg ttgtatggtt aacggcgcag gcctggcgat gggcaccatg gacatcgtta
     8761 aactgcacgg cggcgaacca gctaacttcc tggacgttgg cggcggcgca accaaagaac
     8821 gtgtaaccga agcgttcaaa atcatcctct ctgacgacaa agtgaaagcc gttctggtta
     8881 acatcttcgg cggtatcgtt cgttgcgacc tgatcgctga cggtatcatc ggcgcggtag
     8941 cggaagtggg tgttaacgta ccggtcgtgg tacgtctgga aggtaacaac gccgaactcg
     9001 gcgcgaagaa actggctgac agcggcctga atattattgc agcaaaaggt ctgacggatg
     9061 cagctcagca ggttgttgcc gcagtggagg ggaaataatg tccattttaa ttgataaaaa
     9121 caccaaggtt atctgccagg gctttaccgg tagccagggg actttccact ctgaacaagc
     9181 cattgcatac ggcactaaaa tggttggcgg cgtaacccca ggtaaaggcg gcaccaccca
     9241 cctcggcctg ccggtgttca acaccgtgcg tgaagctgtt gctgctactg gcgctaccgc
     9301 ttctgttatc tacgtaccag caccgttctg caaagactcc attctggaag ctatcgatgc
     9361 aggcatcaaa ctgattatca ccatcactga aggcatcccg acgctggata tgctgaccgt
     9421 gaaagtgaag ctggatgaag caggcgttcg tatgatcggc ccgaactgcc caggcgttat
     9481 cactccgggt gaatgcaaaa tcggtatcca gcctggtcac attcacaaac cgggtaaagt
     9541 gggtatcgtt tcccgttccg gtacgctgac ctatgaagcg gttaaacaga ccacggatta
     9601 cggtttcggt cagtcgacct gtgttggtat cggcggcgat ccgatccctg gctctaactt
     9661 tatcgacatc ctcgaaatgt tcgaaaaaga tccgcagacc gaagcgatcg tgatgatcgg
     9721 tgagatcggc ggtagcgctg aagaagaagc agcggcgtac atcaaagagc acgttaccaa
     9781 gccagttgta ggttacatcg ctggtgtgac tgcgccgaaa ggcaaacgta tgggccacgc
     9841 gggtgcgatc attgccggtg ggaaagggac tgcggatgag aaattcgctg ctctggaagc
     9901 cgcaggcgtg aaaaccgttc gcagcctggc ggatatcggt gaagcactga aaactgttct
     9961 gaaataaata tctgtaataa gaaatagccc tcgccgcttc cttctacagg aatggcgaag
    10021 ggctgtcggt ttcgacatgg ttggccatcg tatgatggcc ttttttatgc ttatcgcgat
    10081 gattttcgct gcgctatcag ggtaaattta tagtcatcgg tattaaaagc gttgcggcta
    10141 tattcaaaca cccggccatc aactaaatat ccacgcgata ctttttcaag aatcggctta
    10201 gtctggctga tattaagcag acggctcatt tcttcggttg gcatcagagg aatgatttcc
    10261 tgttcgctac gatcgataac cattttcttc acttcttcaa taaagtgata tttcgaattt
    10321 tccatgacct gccaggtgag atccgggaac aacgcaagcg gcatccaggt ttcttccagc
    10381 gccattggct tttgcttgcg atagcgcacg cgcttcacat gccacacacg atcctgcgcg
    10441 gtgatttgta gctgttgctg aagaaaatcg tcagccggaa tcacttcgaa tatcagaact
    10501 tcactgtgtg tatcgacgtg acggtccgtc agtttttcat caaaactggt taactgaaaa
    10561 atatcgtaat tgacccgctc ttcttttacg taagtcccgc tgccctgaat actttcgagg
    10621 atctgctgtt caactaactg gcgcaaagcc tgacgcaccg taacccggct gacgccaaac
    10681 tctgtttgta gcgctgattc agtgggtaac gcatcgccag gtttaagctc gccacgcgca
    10741 atttgctcac gaatgcgatc agcaatctgt cggtataagg gcttccgtcc catgatttag
    10801 catctcatta atacgaattt agccattatg cccgataaat tcatcctg
//
LOCUS       DAZFLD010000159        10628 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.160, whole genome shotgun sequence.
ACCESSION   DAZFLD010000159 DAZFLD010000000
VERSION     DAZFLD010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10628
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.160"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..90)
                     /gene="yojO"
                     /locus_tag="ACHKBL_003596"
     CDS             complement(<1..90)
                     /gene="yojO"
                     /locus_tag="ACHKBL_003596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YojO"
                     /protein_id="HFO5820413.1"
                     /translation="MHTPIGVKPVAGSKEWQEAWQKRAFAHISN"
     gene            199..693
                     /gene="napF"
                     /locus_tag="ACHKBL_003597"
     CDS             199..693
                     /gene="napF"
                     /locus_tag="ACHKBL_003597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HFO5820414.1"
                     /translation="MKIDASRRGILTGRWRKASNGIRPPWSGDESHFLTHCTRCDACI
                     NACENNILQRGAGGYPSVNFKNNECSFCYACAQACPESLFSPRHTRAWDLLFTIGEAC
                     LAYQSVECRRCQDSCEPMAIIFRPTLSGIYQPQLNSQLCNACGACAASCPVSAITAEY
                     LHVH"
     gene            683..946
                     /gene="napD"
                     /locus_tag="ACHKBL_003598"
     CDS             683..946
                     /gene="napD"
                     /locus_tag="ACHKBL_003598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HFO5820415.1"
                     /translation="MYTNWQVCSLVVQAKSERISDISTQLNAFPGCEVAVSDAPSGQL
                     IVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQEEQGEETP"
     gene            943..3429
                     /gene="napA"
                     /locus_tag="ACHKBL_003599"
     CDS             943..3429
                     /gene="napA"
                     /locus_tag="ACHKBL_003599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic nitrate reductase subunit alpha"
                     /protein_id="HFO5820416.1"
                     /translation="MKLSRRSFMKANAVAAAAAAAGLSVPGVARAVVGQQEAIKWDKA
                     PCRFCGTGCGVLVGTQQGRVVACQGDPDAPVNRGLNCIKGYFLPKIMYGKDRLTQPLL
                     RMKNGKYDKEGEFTPITWDQAFDVMEEKFKTALKEKGPESIGMFGSGQWTIWEGYAAS
                     KLFKAGFRSNNIDPNARHCMASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGSNMAE
                     MHPILWSRITNRRLSNQNVTVAVLSTYQHRSFELADNGIIFTPQSDLVILNYIANYII
                     QNNAINQDFFSKHVNLRKGATDIGYGLRPTHPLEKAAKNPGSDASEPMSFEDYKAFVA
                     EYTLEKTAEMTGVPKDQLEQLAQLYADPNKKVISYWTMGFNQHTRGVWANNLVYNLHL
                     LTGKISQPGCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNEKHRDICEKKWNIPS
                     GTIPAKIGLHAVAQDRALKDGKLNVYWTMCTNNMQAGPNINEERMPGWRDPRNFIIVS
                     DPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWRQQVQAPGEAKSDLWQLVQFS
                     RRFKTEEVWPEELLAKKPELRGKTLYEVLYATPEVSKFPLSELAEDQLNDESRELGFY
                     LQKGLFEEYAWFGRGHGHDLAPFDDYHKARGLRWPVVNGKETQWRYSEGNDPYVKAGE
                     GYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLSTGRVLEHWHTGSMTRRVPELHRA
                     FPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISIVETRGRNRPPQGLVYMPFFDAA
                     QLVNKLTLDATDPLSKETDFKKCAVKLEKV"
     gene            3436..4131
                     /gene="napG"
                     /locus_tag="ACHKBL_003600"
     CDS             3436..4131
                     /gene="napG"
                     /locus_tag="ACHKBL_003600"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HFO5820417.1"
                     /translation="MSRSAKPQNGRRRFLRDVVRTAGGLAAVGVALGLQQQTARASGV
                     RLRPPGAINENAFASACVRCGQCVQACPYDTLKLATLASGLSAGTPYFVARDIPCEMC
                     EDIPCAKVCPSGALDREIESIDDARMGLAVLVDQENCLNFQGLRCDVCYRECPKIDEA
                     ITLELERNTRTGKHARFLPTVHSDACTGCGKCEKVCVLEQPAIKVLPLSLAKGELGHH
                     YRFGWLEGNNGKS"
     gene            4118..4981
                     /gene="napH"
                     /locus_tag="ACHKBL_003601"
     CDS             4118..4981
                     /gene="napH"
                     /locus_tag="ACHKBL_003601"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HFO5820418.1"
                     /translation="MANRKRDAGREALEKKGWWRSHRWLVLRRLCQFFVLGMFLSGPW
                     FGVWILHGNYSSSLLFDTVPLTDPLMTLQSLASGHLPASVALTGAVIITVLYALAGKR
                     LFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVVILLGSALTGTLIWEWI
                     NPVSLMGRSLVMGFGSGALLILALFLFDLLVVEHGWCGHICPVGALYGVLGSKGVITV
                     AATDRQKCNRCMDCFHVCPEPHVLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFT
                     ITTRWSSGAKS"
     gene            4978..5427
                     /gene="napB"
                     /locus_tag="ACHKBL_003602"
     CDS             4978..5427
                     /gene="napB"
                     /locus_tag="ACHKBL_003602"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HFO5820419.1"
                     /translation="MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQE
                     GAIRMPKEQDRMPLNYVNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGAPRISP
                     THFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK"
     gene            5437..6039
                     /gene="napC"
                     /locus_tag="ACHKBL_003603"
     CDS             5437..6039
                     /gene="napC"
                     /locus_tag="ACHKBL_003603"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type protein NapC"
                     /protein_id="HFO5820420.1"
                     /translation="MGNSDRKPGLIKRLWKWWRTPSRLALGTLLLIGFVGGIVFWGGF
                     NTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNRSGVRATCPDCHVPHEFVPKMIR
                     KLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
                     QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF"
     gene            6052..6675
                     /gene="ccmA"
                     /locus_tag="ACHKBL_003604"
     CDS             6052..6675
                     /gene="ccmA"
                     /locus_tag="ACHKBL_003604"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HFO5820421.1"
                     /translation="MGMLEARELLCERDERTLFSGLSFTLNAGEWVQITGSNGAGKTT
                     LLRLLTGLSRPDAGDVLWQGQPLHQVRDSYHQNLLWIGHQPGIKTRLTALENLHFYHR
                     DGDTAQCLEALAQAGLAGFEDIPVNQLSAGQQRRVALARLWLTRATLWILDEPFTAID
                     VNGVDRLTQRMAQHTEQGGIVILTTHQPLNVAENKIRRISLTQTRGE"
     gene            6672..7334
                     /gene="ccmB"
                     /locus_tag="ACHKBL_003605"
     CDS             6672..7334
                     /gene="ccmB"
                     /locus_tag="ACHKBL_003605"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HFO5820422.1"
                     /translation="MMFWRIFRLELRVAFRHSAEIANPLWFFLIVITLFPLSIGPEPQ
                     LLARIAPGIIWVAALLSSLLALERLFRDDLQDGSLEQLMLLPLPLPAVVLAKVMAHWM
                     VTGLPLLILSPLVAMLLGMDVYGWQVMALTLLLGTPTLGFLGAPGVALTVGLKRGGVL
                     LSILVLPLTIPLLIFATAAMDAASMHLPVDGYLAILGALLAGTATLSPFATAAALRIS
                     IQ"
     gene            7376..8113
                     /gene="ccmC"
                     /locus_tag="ACHKBL_003606"
     CDS             7376..8113
                     /gene="ccmC"
                     /locus_tag="ACHKBL_003606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmC"
                     /protein_id="HFO5820423.1"
                     /translation="MWKTLHQLAIPPRLYQICGWFIPWLAIASVVVLTVGWIWGFGFA
                     PADYQQGNSYRIIYLHVPAAIWSMGIYASMAVAAFIGLVWQMKMANLAVAAMAPIGAV
                     FTFIALVTGSAWGKPMWGTWWVWDARLTSELVLLFLYVGVIALWHAFDDRRLAGRAAG
                     ILVLIGVVNLPIIHYSVEWWNTLHQGSTRMQQSIDPAMRSPLRWSIFGFLLLSATLTL
                     MRMRNLILLMEKRRPWVSELILKRGRK"
     gene            8110..8319
                     /gene="ccmD"
                     /locus_tag="ACHKBL_003607"
     CDS             8110..8319
                     /gene="ccmD"
                     /locus_tag="ACHKBL_003607"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HFO5820424.1"
                     /translation="MTPAFASWNEFFAMGGYAFFVWLAVVMTVIPLVILVVHSVMQHR
                     AILRGVAQQRAREARLRAAQQQEAA"
     gene            8316..8795
                     /gene="ccmE"
                     /locus_tag="ACHKBL_003608"
     CDS             8316..8795
                     /gene="ccmE"
                     /locus_tag="ACHKBL_003608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HFO5820425.1"
                     /translation="MNIRRKNRLWIACAVLAGLALTIGLVLYALRSNIDLFYTPGEIL
                     YGKRETQQMPEVGQRLRVGGMVMPGSVQRDPNSLKVTFTIYDAEGSVDVSYEGILPDL
                     FREGQGVVVQGELEKGNHILAKEVLAKHDENYTPPEVEKAMEANHRRPASVYKDPAS"
     gene            8792..>10628
                     /gene="ccmF"
                     /locus_tag="ACHKBL_003609"
     CDS             8792..>10628
                     /gene="ccmF"
                     /locus_tag="ACHKBL_003609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis heme lyase CcmF"
                     /protein_id="HFO5820426.1"
                     /translation="MMPEIGNGLLCLALGIALLLSVYPLWGVARGDARMMASSRLFAW
                     LLFMSVAGAFLVLVNAFVVNDFTVTYVASNSNTQLPVWYRVAATWGAHEGSLLLWVLL
                     MSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILFTSNPFSRTLPNFPIEGRD
                     LNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIASLLSGRLDSTYARFTRPWTLAAWIF
                     LTLGIVLGSAWAYYELGWGGWWFWDPVENASFMPWLVGTALMHSLAVTEQRASFKAWT
                     LLLAISAFSLCLLGTFLVRSGVLVSVHAFASDPARGMFILAFMVLVIGGSLLLFAARG
                     HKVRSRVNNALWSRESLLLANNVLLVAAMLVVLLGTLLPLVHKQLGLGSISIGEPFFN
                     TMFTWLMVPFALLLGVGPLVRWGRDRPRKIRNLLIIAFISTLVLSLLLPWLFESKVVA
                     MTVLGLAMACWIAMLAIAEAALRISRGTKTTFSYWGMVAAHLGLAVTIVGIAFSQNYS
                     VERDVRMKSGDSVDIHEYRFTFRDVKEVTGPNWRGGVATIGVTRNGKPETVLYAEKRY
                     YNTAGSMMTEAAIDGGITRDLYAALGEELENGAWAVRLYYKPFVRW"
BASE COUNT         2171 a         2724 c         3197 g         2536 t
ORIGIN      
        1 atttgaaatg tgagcaaaag cccgtttttg ccacgcttcc tgccactctt ttgatcctgc
       61 tacaggtttt accccgatcg gggtatgcat ctttgacaca tcctttaata tcttagcggc
      121 tataaaaatg gcttattaat tatgcggttt atttggtcgc tctcaatttt cagagcgcgt
      181 taatgatgga aggtcaatgt gaagattgat gcatcccgtc ggggcatact cactggtcgc
      241 tggcgcaaag ccagtaacgg tatccgtccg ccctggtccg gtgatgaatc tcattttctg
      301 acccattgca ctcgctgcga cgcctgtatt aatgcttgtg aaaacaacat tctgcaacgc
      361 ggcgcgggtg gctatccgag cgttaatttc aaaaataacg agtgcagctt ctgttacgcc
      421 tgcgcgcagg cttgccccga atcgctattt tctccgcgcc acaccagggc ctgggatttg
      481 ctgttcacca tcggggaggc gtgcctggcg tatcagtcag ttgaatgccg ccgctgtcag
      541 gatagctgtg agccaatggc aattatcttt cgcccgacgc tctccgggat ctaccagccg
      601 caacttaata gccaactttg caacgcatgt ggagcatgtg cagccagctg tccggtatca
      661 gccataactg cggagtatct ccatgtacac taactggcaa gtttgcagcc tggtcgtgca
      721 ggccaaaagc gaacgaattt cagacatcag cacccaactg aacgcctttc ccggctgtga
      781 agttgctgtc agcgacgcgc cgagcggcca gttgattgtg gtggtggaag cagaagacag
      841 cgaaacgctg atccaaacca ttgagtcagt acgcaacgta gagggcgtgc tggcggtgtc
      901 gctggtttat caccagcagg aagagcaagg tgaggaaaca ccatgaaact cagtcgtcgt
      961 agctttatga aagctaacgc cgttgcggcc gctgcggcgg ctgccggtct cagcgtgccg
     1021 ggcgttgccc gcgccgttgt tggtcagcag gaagccatta aatgggataa agcgccgtgc
     1081 cgtttctgcg gtacgggttg cggcgttctg gtcggaacgc agcaggggcg tgtggtggcc
     1141 tgtcagggcg acccggacgc accggttaac cgtggtctga actgcattaa gggctatttc
     1201 ctgcccaaaa tcatgtacgg taaagaccgt ttgacgcagc cgctgctgcg tatgaaaaac
     1261 ggtaaatatg acaaagaagg cgaatttacc ccaatcacct gggatcaagc cttcgatgtg
     1321 atggaagaga aattcaaaac cgccctgaaa gaaaaagggc cggaatcgat cggtatgttc
     1381 ggttctggtc agtggactat ctgggaaggt tatgccgcgt ccaagctgtt taaagcggga
     1441 ttccgttcga acaacatcga cccgaacgcg cgtcactgta tggcgtcggc ggtagttggc
     1501 tttatgcgta cctttggtat ggatgagccg atgggctgct atgacgacat cgagcaggct
     1561 gacgcgtttg tgctgtgggg ctccaacatg gcggagatgc acccgatcct ctggtcgcgc
     1621 atcactaacc gtcgtctctc caaccagaac gtcaccgtgg cggtgctttc tacctaccag
     1681 catcgtagct tcgagctggc ggataacggc atcatcttta cgccgcaatc tgacctggtg
     1741 atcctgaact atatcgccaa ctatatcatt caaaacaatg cgataaatca ggacttcttc
     1801 agcaaacacg ttaacctgcg caaaggggcg acggacatcg gctacggttt acgtccgacc
     1861 catccgctgg aaaaagcagc gaagaatccg ggttctgacg cctccgaacc gatgagcttt
     1921 gaagattaca aagccttcgt tgctgaatat acgctggaaa aaaccgccga aatgaccggc
     1981 gtgccgaaag accagttaga acaactggcg cagctgtatg ccgatccgaa caagaaagtc
     2041 atctcctact ggacgatggg cttcaaccag catacccgtg gtgtgtgggc caacaacctg
     2101 gtctacaacc tgcacctgct gaccggcaaa atttcccagc cgggttgcgg tccgttctcc
     2161 ctgaccgggc agccttccgc gtgtggtact gcgcgtgaag tgggcacctt tgctcaccgt
     2221 ctgcctgcgg acatggtggt gactaacgag aaacatcgtg atatttgcga gaagaagtgg
     2281 aatatcccga gtggcaccat tccggcgaaa atcggtctgc acgcggtggc acaagaccgt
     2341 gcgctgaaag acggcaagct gaatgtttac tggaccatgt gtaccaacaa catgcaggcc
     2401 gggccgaaca ttaatgaaga gcgtatgcca ggctggcgcg atccgcgcaa ctttatcatc
     2461 gtttccgatc cgtatccgac agtcagtgcg ctggcagcgg acttgatcct gccgaccgca
     2521 atgtgggtag agaaagaggg ggcttacggt aacgccgaac gccgtactca attctggcgt
     2581 cagcaggtac aggcaccggg cgaagcgaaa tcggatctct ggcagttagt ccagttctcc
     2641 cgccgcttca aaactgaaga agtatggccg gaagagctgc tggcgaagaa accggaactg
     2701 cgtggcaaaa cgctgtacga agttctgtat gccaccccgg aagtgagcaa attcccgcta
     2761 tccgaactgg cggaagatca gctgaacgat gaatcccgcg agctgggctt ctatctgcaa
     2821 aaagggctgt tcgaagagta cgcatggttt ggtcgcggtc acggtcacga tctcgcgccg
     2881 ttcgatgact accacaaagc acgcggtctg cgctggccgg tggtgaacgg taaagaaacg
     2941 cagtggcgtt acagcgaagg taacgacccg tacgtgaaag cgggcgaagg ctacaagttc
     3001 tacggtaaac cggatggcaa agcggtgatc ttcgcgctgc cgttcgaacc tgcggcggaa
     3061 gcaccggatg aagagtacga cctgtggctc tctaccggac gcgtcctgga gcactggcat
     3121 accggcagta tgactcgccg tgtaccggaa ctgcaccgcg ccttcccgga agcggtcctg
     3181 tttattcacc cgctggatgc gaaagcgcgc gatctgcgcc gtggcgacaa agtgaaagtg
     3241 gtttctcgcc gtggcgaagt gatctcgatt gtcgaaacgc gcggtcgtaa ccgtccgccg
     3301 cagggcctgg tgtacatgcc gttcttcgat gccgcacagc tggttaacaa actgacgctg
     3361 gatgcgaccg atccgctctc gaaagagacg gacttcaaga agtgcgcggt caaactggag
     3421 aaggtgtaag ccattatgtc ccggtcagcg aaacctcaaa atggtcgccg ccgctttctg
     3481 cgcgatgtcg ttcgcacagc tggcgggctg gctgccgtgg gtgtggcgct ggggttacaa
     3541 cagcaaaccg cacgcgcatc tggcgtgcgg ttgcgcccgc ccggagccat aaacgagaac
     3601 gcctttgcca gtgcctgtgt gcgttgcggt cagtgtgttc aggcttgccc ttacgacacc
     3661 ttaaaactgg cgacgctggc ctctggcctg tcggcgggca cgccatattt tgtcgcacgg
     3721 gatattcctt gcgaaatgtg tgaggacatt ccgtgcgcca aagtgtgccc gagcggtgcg
     3781 ctggatcgtg agatcgaatc gatcgacgac gcgcgaatgg ggctggcggt actggtggac
     3841 caggaaaact gtctcaactt tcaggggctg cgctgcgatg tttgttatcg cgaatgcccg
     3901 aaaattgatg aggccatcac cctggagctg gagcgcaaca cgcgtaccgg taagcacgcc
     3961 cgctttctgc cgacggttca cagcgacgcc tgtaccggtt gcggtaagtg cgaaaaagtg
     4021 tgcgtgctgg aacaaccggc aatcaaggtg ttaccgctgt cactagcgaa aggggagtta
     4081 ggtcaccatt accgcttcgg ctggctggag gggaacaatg gcaaatcgta aacgtgacgc
     4141 cgggcgcgag gcgctggaga aaaaaggctg gtggcgcagt caccgttggt tagtgttacg
     4201 tcgcctttgt cagttcttcg tgctggggat gtttttgagc ggtccgtggt ttggtgtgtg
     4261 gatcttgcac ggcaactaca gcagtagcct gttattcgac accgttccgc tgaccgatcc
     4321 gctgatgacg ctgcaaagtc ttgccagcgg tcatctgccg gcctcggtgg cgctgaccgg
     4381 agcggtgatt atcaccgtgc tctacgccct ggcaggaaag cgattatttt gcagctgggt
     4441 ctgcccgctg aacccgatta ccgacttagc gaactggtta cgcaggcgct ttgacctcaa
     4501 tcagtctgcg acgatcccac gccacattcg ctacgtgctg ttggtggtca ttctgctggg
     4561 atcggcctta accggcacgc tcatctggga atggatcaac cccgtttccc tgatggggcg
     4621 cagtctggta atgggattcg gcagcggcgc gctgcttatt ctcgcgctgt ttttatttga
     4681 tctactggtc gttgaacacg gctggtgcgg gcacatttgt ccggttggcg cgctgtatgg
     4741 cgtgctgggt agcaaaggtg tgattaccgt tgcggccacc gatcggcaga aatgtaaccg
     4801 ctgtatggat tgttttcatg tttgcccgga accgcatgtg ctacgtgccc cggtgctgga
     4861 tgaacaaagc ccggtgcagg tcaccagccg cgattgcatg acgtgcggtc gctgcgtgga
     4921 tgtctgttct gaggatgtat ttacaataac tacacgatgg agttcgggag cgaaatcatg
     4981 aaaagccatg acctgaagaa agcgctgtgt caatggacgg cgatgctggc cctggtggtg
     5041 agcggcgcgg tttgggcggc taatggcgtc gattttagcc agtcgccgga agtatccggg
     5101 acgcaggaag gggccattcg gatgccaaaa gagcaggatc ggatgccgct gaactatgtg
     5161 aatcagccgc caatgatccc gcatagcgtt gaaggttatc aggtaacgac caataccaac
     5221 cgctgcttgc agtgccacgg tgtcgaaagc tatcgcacca ctggcgcgcc gcgtatcagt
     5281 cctactcact ttatggacag cgacggcaaa gtgggcgcag aagtggctcc gcgtcgttat
     5341 ttctgtctgc aatgtcacgt accgcaggcc gataccgcgc caatcgtggg gaataccttt
     5401 accccatcaa aaggttacgg gaaataagag gttattatgg gaaattctga ccgtaagcct
     5461 ggtctgatta agcgcctgtg gaaatggtgg cgtaccccca gccgtctggc gctggggacg
     5521 ctgctgttga tcggttttgt tggcggcatc gtcttctggg gcggctttaa caccgggatg
     5581 gaaaaagcca ataccgaaga gttctgcatt agctgccacg aaatgcgtaa cacggtgtat
     5641 caggaataca tggattccgt gcactacaac aaccgtagcg gcgttcgtgc gacctgtccg
     5701 gattgtcacg ttccgcacga gtttgtgccg aagatgatac gcaagctcaa agcaagtaaa
     5761 gagctgtatg gtaaaatttt tggcgttatt gacacgccgc agaaatttga agctcatcgc
     5821 ctgacgatgg cacagaatga gtggcggcgc atgaaggaca ataactcgca ggagtgccgt
     5881 aactgtcaca acttcgagta tatggataca accgcccaga aatcggttgc cgcgaagatg
     5941 catgaccagg cggtgaaaga tgggcaaacc tgtattgatt gccataaagg gatagcgcac
     6001 aagctgcccg atatgcgcga agtcgagcca ggtttttaac agggttattg cgtgggtatg
     6061 cttgaagcca gagagttact ttgcgagcgg gatgaacgaa ccttatttag tggcttgtca
     6121 tttacgctga acgcaggaga gtgggtacaa atcaccggta gcaacggcgc ggggaagaca
     6181 acgcttctcc gtttgctgac ggggttgtct cgccctgatg cgggtgacgt tctctggcaa
     6241 gggcagccct tgcatcaggt acgcgacagc taccatcaaa acctgttatg gatagggcat
     6301 cagccgggga tcaaaacccg gctgacggcg ttagaaaatc tgcactttta tcatcgcgat
     6361 ggcgataccg cacaatgtct ggaagccctg gcgcaggccg ggcttgccgg attcgaagat
     6421 attccggtaa atcaactttc tgccgggcaa caacgccgcg tcgctttagc gcgtctgtgg
     6481 ctgacccgtg ccacgttatg gatcctcgac gagcctttta ccgcgattga cgttaacggc
     6541 gtcgatcgtc tgacccagcg tatggcgcag catacggagc agggggggat tgtgattctg
     6601 actacccacc agccgctcaa cgttgctgaa aataaaattc gccgcatttc actgacgcaa
     6661 acgaggggcg aataatgttc tggcgcattt tccgtcttga gctgcgcgta gcgtttcgcc
     6721 atagcgccga aatcgccaac ccgctgtggt tcttcctgat tgtcattacc ctttttccgc
     6781 tcagtatcgg tccggagccg caactgctgg cgcgtattgc gccgggcatt atctgggttg
     6841 ctgcgctgct ttcatccttg ctggcactgg agcgactgtt ccgtgacgat ttgcaggacg
     6901 gcagtcttga acaattgatg ttgttgccgt tacccttgcc cgccgttgtg ctggcgaagg
     6961 tgatggcgca ctggatggta accggtctgc cgttactcat cctttcgccc ctggtagcaa
     7021 tgctactggg aatggatgtt tatggctggc aagtgatggc gttaacgcta ctgctgggaa
     7081 cccctacgct tggctttctt ggtgcgccgg gcgtggcgct gacagtggga cttaagcgcg
     7141 gtggtgtgct gctcagcata ctggtgttac cgctgactat cccattactc atctttgcca
     7201 ccgccgcgat ggacgcggct tctatgcatt tgcccgttga tgggtatctg gcaattttag
     7261 gcgcgttgct ggcaggcacc gcgacattaa gtccttttgc gacggcggca gcgttacgaa
     7321 tcagcattca ataacgctgg attactgatt tttgttcgag tctggtatcg aaactatgtg
     7381 gaaaacactg catcaactgg cgatcccacc acggctgtat caaatctgtg gctggtttat
     7441 accgtggctg gcaattgcca gtgtggtcgt gcttaccgtc ggctggatct ggggattcgg
     7501 ttttgctccg gctgattatc agcagggaaa tagctaccgc attatctacc tgcatgtgcc
     7561 tgcggcgatc tggtcgatgg gcatttatgc atcaatggcg gtggcggcgt ttattggcct
     7621 tgtctggcag atgaaaatgg ccaacctggc ggtggcggcg atggccccca ttggtgccgt
     7681 gtttaccttt attgccctgg ttaccggctc tgcatgggga aaaccgatgt ggggcacctg
     7741 gtgggtatgg gatgcacgcc tgacttctga actggtactg ctgtttttgt atgtgggtgt
     7801 gattgccctg tggcacgcct tcgacgaccg ccgtctggcg ggccgtgcgg caggtattct
     7861 ggtgctgatt ggcgtggtga atctgccgat tattcattac tccgtggagt ggtggaacac
     7921 cctgcatcag ggatcaacgc ggatgcaaca aagtatcgat ccggcgatgc gttcgccgct
     7981 gcgctggtcg atttttggct tcctgctgct gtctgccacg ctgacgctga tgcggatgcg
     8041 taatttgatt ttgctgatgg aaaaacgccg tccgtgggtg agtgaactga tactgaaaag
     8101 aggccgtaaa tgacccctgc atttgcttcc tggaatgaat ttttcgcaat gggcggttac
     8161 gccttttttg tctggctggc ggtggtgatg accgttattc cgctggtgat tttggtcgtg
     8221 cactcggtga tgcagcatcg cgcaattctg cgtggcgtgg cgcaacagcg ggcgcgtgag
     8281 gcgcgtttac gtgctgcgca acagcaggag gctgcatgaa tattcgccgt aaaaaccgct
     8341 tgtggattgc ctgtgccgtg ctggcggggc tggcgctgac tatcggtctg gtgctatatg
     8401 cgctgcgctc gaatatcgat ctcttttata cgccggggga gattctctac ggcaagcgtg
     8461 aaactcagca gatgccggaa gtcggtcagc gtctgcgcgt tggcgggatg gtgatgccgg
     8521 ggagtgtgca gcgcgatccc aattcgctaa aagtaacttt caccatttac gacgctgaag
     8581 gttcagtaga tgtctcttac gaaggcattt tgccggatct gttccgtgaa gggcagggcg
     8641 ttgtggtgca gggcgagctg gaaaaaggca atcatatcct cgcgaaagaa gtgctggcga
     8701 aacacgatga aaactacacg ccgccagaag ttgagaaagc gatggaagct aaccaccgtc
     8761 gcccggcgag tgtttataag gacccagcat catgatgcca gaaattggta acgggctgct
     8821 gtgtctggcg ctaggaattg cgctgctgct gtctgtgtat ccgctatggg gcgtggcgcg
     8881 cggggacgcg cgcatgatgg cgtcttcccg cttgtttgcc tggctgctgt ttatgtctgt
     8941 ggctggcgca tttctggtgc tggtcaatgc tttcgtggtc aacgacttta ccgtcaccta
     9001 tgttgccagc aactccaata cccagcttcc ggtgtggtat cgcgtggcgg ctacctgggg
     9061 cgcgcatgaa ggctcgctcc tgctgtgggt gctactgatg agcggctgga cctttgcggt
     9121 agcgattttt agtcagcgta ttccgctgga tattgtggcc cgcgtactgg cgataatggg
     9181 gatggtcagt gtcggctttt tgctgttcat tctctttacc tctaacccgt tctcacgcac
     9241 gctgccgaat ttcccgattg aagggcgcga tcttaacccg ctgttgcagg atccggggct
     9301 gatcttccat ccgcctctgc tctatatggg gtacgtcggt ttctcggtgg cgtttgcttt
     9361 cgccattgct tctttgttga gtgggcgtct ggacagcacc tatgcgcgtt ttacccgccc
     9421 gtggacgctg gcggcgtgga tcttcttgac gctcggcatt gtgctcggtt ccgcatgggc
     9481 ctattacgaa ctcggctggg gcggctggtg gttctgggat ccggtagaaa acgcctcgtt
     9541 tatgccgtgg ctggtgggga ctgcgctgat gcactcactg gcggtcactg aacaacgcgc
     9601 cagcttcaaa gcgtggacat tactgctggc aatcagtgcc ttctcgttgt gtctgttggg
     9661 gactttcctc gtgcgttccg gcgtgctggt atcggtacac gcgtttgcgt ctgatccggc
     9721 gcgcggtatg tttatcctcg cctttatggt gttggtgatt ggcggttcgc tgctgctgtt
     9781 tgccgcgcgt ggacacaaag ttcgctcacg cgtaaacaat gcgctgtggt cgcgggaatc
     9841 tctgctgtta gcgaacaacg ttttgctggt cgccgcgatg ctggtggtat tactggggac
     9901 gctgctgccg ctggtgcaca agcaactggg actgggcagt atttcgattg gcgaaccgtt
     9961 cttcaacacc atgtttacct ggctgatggt gccgtttgcg ctgctgcttg gtgtcggtcc
    10021 tctggtgcgc tgggggcgcg atcgcccacg taaaatccgc aatttattga ttatcgcctt
    10081 catctcaacg ctggtgctgt cgctgctgct gccgtggctg ttcgaaagca aagttgtggc
    10141 gatgacggtg ctcggcctgg caatggcctg ctggattgcg atgctggcaa ttgcggaagc
    10201 tgcgctgcgt atttcacgtg gcacgaaaac caccttcagt tattggggaa tggtggcggc
    10261 tcacctgggg ctggcagtga caattgttgg tattgccttt agccagaact atagcgttga
    10321 gcgtgatgtg cgcatgaagt ccggcgatag cgtcgatatt catgaatatc gcttcacctt
    10381 ccgtgatgtc aaagaggtga ctggtccgaa ctggcgtggc ggtgtggcga ctatcggcgt
    10441 aacgcgaaat ggcaagccgg aaacagtgct gtatgcggaa aaacgttatt acaacactgc
    10501 cgggtcgatg atgaccgaag cggcaattga cggcggcatc acgcgtgacc tgtacgcggc
    10561 cctcggtgaa gagctggaaa acggcgcgtg ggccgtgcgt ctttactaca aaccgtttgt
    10621 tcgctgga
//
LOCUS       DAZFLD010000160        10608 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.161, whole genome shotgun sequence.
ACCESSION   DAZFLD010000160 DAZFLD010000000
VERSION     DAZFLD010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10608
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.161"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            60..626
                     /gene="yceJ"
                     /locus_tag="ACHKBL_003610"
     CDS             60..626
                     /gene="yceJ"
                     /locus_tag="ACHKBL_003610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HFO5820427.1"
                     /translation="MSFTNTPERYGVISAAFHWLSAIIVYGMFALGLWMVTLSYYDGW
                     YHQAPELHKSIGILLMMGLVIRVLWRVISPPPGPLQSYSPMTRLGAKAGHLALYLLLF
                     AIGISGYLISTADGKPISVFGWFDVPATLADAGAQADFAGALHFWLAWSVVVLSVMHG
                     FMALKHHFIDKDDTLKRMLGKSSSDYGV"
     gene            630..1205
                     /gene="yceI"
                     /locus_tag="ACHKBL_003611"
     CDS             630..1205
                     /gene="yceI"
                     /locus_tag="ACHKBL_003611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HFO5820428.1"
                     /translation="MKKSLLGLTFASLMFSAGSAVAADYKIDKEGQHAFVNFRIQHLG
                     YSWLYGTFKDFDGTFTFDEKNPAADKVNVTINTTSVDTNHAERDKHLRSADFLNTAKY
                     PQATFTSTSVKKDGDELDITGDLTLNGVTKPVTLEAKLIGQGDDPWGGKRAGFEAEGK
                     IKLKDFNIKTDLGPASQEVDLIISVEGVQQK"
     gene            complement(1247..2299)
                     /gene="trhO"
                     /locus_tag="ACHKBL_003612"
     CDS             complement(1247..2299)
                     /gene="trhO"
                     /locus_tag="ACHKBL_003612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HFO5820429.1"
                     /translation="MPVLHNRISNDALKAKMLAESEPRTTISFYKYFHIADPKATRDA
                     LYQLFTALDVFGRVYLAHEGINAQISVPASNVETFRAQLYAFDPALEGLRLNIALDDD
                     GKSFWVLRMKVRDRIVADGIDDPHFDASNVGEYLQAAEVNAMLDDPDALFIDMRNHYE
                     YEVGHFENALEIPADTFREQLPKAVEMMQAHKDKKIVMYCTGGIRCEKASAWMKHNGF
                     NKVWHIEGGIIEYARKAREQGLPVRFIGKNFVFDERMGERISDEIIAHCHQCGAPCDS
                     HTNCKNDGCHLLFIQCPVCAEKYKGCCSEICCEESALPPEEQRRRRAGRENGNKIFNK
                     SRGRLNTTLGIPDPTE"
     gene            2524..3444
                     /gene="lpxL"
                     /locus_tag="ACHKBL_003613"
     CDS             2524..3444
                     /gene="lpxL"
                     /locus_tag="ACHKBL_003613"
                     /EC_number="2.3.1.241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) lauroyltransferase"
                     /protein_id="HFO5820430.1"
                     /translation="MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPVIYRLGCGL
                     GKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVVKNFESVGMGLMETGMAWFWPDR
                     RIARWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGMQEPGIGVYRPNDNPL
                     IDWLQTWGRLRSNKSMLDRKDLKGMIKTLKKGEVVWYAPDHDYGPRSSVFVPLFAVEQ
                     AATTTGTWMLARMSGACLVPFVPRRKPDGKGYQLIMLPPECSPPLDDAETTAAWMNKV
                     VEKCIMMAPEQYMWLHRRFKTRPEGVPSRY"
     gene            3616..4842
                     /gene="mdtG"
                     /locus_tag="ACHKBL_003614"
     CDS             3616..4842
                     /gene="mdtG"
                     /locus_tag="ACHKBL_003614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtG"
                     /protein_id="HFO5820431.1"
                     /translation="MSPCENDTPINWKRNLIVAWLGCFLTGAAFSLVMPFLPLYVEQL
                     GVTGHSALNMWSGIVFSITFLFSAIASPFWGGLADRKGRKLMLLRSALGMGIVMVLMG
                     LAQNIWQFLILRALLGLLGGFVPNANALIATQVPRNKSGWALGTLSTGGVSGALLGPM
                     AGGLLADSYGLRPVFFITASVLILCFFVTLFCIREKFQPVSKKEMLHMREVVTSLKNP
                     KLVLSLFVTTLIIQVATGSIAPILTLYVRELAGNVSNVAFISGMIASVPGVAALLSAP
                     RLGKLGDRIGPEKILITALIFSVLLLIPMSYVQTPLQLGILRFLLGAADGALLPAVQT
                     LLVYNSSNQIAGRIFSYNQSFRDIGNVTGPLMGAAISANYGFRAVFLVTAGVVLFNAV
                     YSWNSLRRRRIPQVSN"
     gene            4925..5299
                     /gene="msyB"
                     /locus_tag="ACHKBL_003615"
     CDS             4925..5299
                     /gene="msyB"
                     /locus_tag="ACHKBL_003615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HFO5820432.1"
                     /translation="MTMYATLEEAIDAAREEFLADNPGIDAEDANVQQFNAQKYVLQD
                     GDIMWQVEFFADEGEEGECLPMLSGEAAQSVFDGDYDEIEIRQEWQEENTLHEWDEGE
                     FQLEPPLDTEEGRAAADEWDER"
     gene            complement(5300..5527)
                     /gene="yceK"
                     /locus_tag="ACHKBL_003616"
     CDS             complement(5300..5527)
                     /gene="yceK"
                     /locus_tag="ACHKBL_003616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HFO5820433.1"
                     /translation="MRLIVMSIMVTLLSGCGSIISRTIPGQGHGNQYYPGVQWDVRDS
                     AWRYVTILDLPFSLVFDTLLLPIDIHHGPYE"
     gene            complement(5699..8242)
                     /gene="mdoH"
                     /locus_tag="ACHKBL_003617"
     CDS             complement(5699..8242)
                     /gene="mdoH"
                     /locus_tag="ACHKBL_003617"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HFO5820434.1"
                     /translation="MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDAEHRTWARED
                     DSPQGSVKARLEQAWPDSLADGQLIKDDEGRDQLKAMPEAKRSSMFPDPWRTNPVGRF
                     WDRLRGRDVTPRYLARLTKEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTIL
                     PYQGWALINPMDMVGQDVWVSFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQ
                     LLIGRDKYSISASTVGDEPLNPEHRTALIMPICNEDVNRVFAGLRATWESVKATGNAK
                     HFDVYILSDSYNPDICVAEQKAWMELIAEVGGEGQIFYRRRRRRVKRKSGNIDDFCRR
                     WGSQYSYMVVLDADSVMTGDCLCGLVRLMEANPNAGIIQSSPKASGMDTLYARCQQFA
                     TRVYGPLFTAGLHFWQLGESHYWGHNAIIRVKPFIEHCALAPLPGEGSFAGSILSHDF
                     VEAALMRRAGWGVWIAYDLPGSYEELPPNLLDELKRDRRWCHGNLMNFRLFLVKGMHP
                     VHRAVFLTGVMSYLSAPLWFMFLALSTALQVVHALTEPQYFLQPRQLFPVWPQWRPEL
                     AIALFASTMVLLFLPKLLSILLIWCKGTKEYGGFWRVTLSLLLEVLFSVLLAPVRMLF
                     HTVFVVSAFLGWEVVWNSPQRDDDSTSWGEAFKRHGSQLLLGLVWAVGMAWLDLRFLF
                     WLAPIVFSLILSPFVSVISSRATVGLRTKRWKLFLIPEEYSPPQVLVDTDRFLEMNRQ
                     RSLDDGFMHAVFNPSFNALATAMATARHRASKVLEIARDRHVEQALNETPEKLNRDRR
                     LVLLSDPVTMARLHFRVWNSPERYSSWVSYYEGIKLNPLALRKPDAASQ"
     gene            complement(8235..9770)
                     /gene="mdoG"
                     /locus_tag="ACHKBL_003618"
     CDS             complement(8235..9770)
                     /gene="mdoG"
                     /locus_tag="ACHKBL_003618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoG"
                     /protein_id="HFO5820435.1"
                     /translation="MMKMRWLSAAVMLTLYTSSSWAFSIDDVAKQAQSLAGKGYEAPK
                     SNLPSVFRDMKYADYQQIQFNHDKAYWNNLKTPFKLEFYHQGMYFDTPVKINEVTATA
                     VKRIKYSPDYFTFGDVQHDKDTVKDLGFAGFKVLYPINSKDKNDEIVSMLGASYFRVI
                     GAGQVYGLSARGLAIDTALPSGEEFPRFKEFWIERPKPTDKRLTIYALLDSPRATGAY
                     KFVVMPGRDTVVDVQSKIYLRDKVGKLGVAPLTSMFLFGPNQPSPANNYRPELHDSNG
                     LSIHAGNGEWIWRPLNNPKHLAVSSFSMENPQGFGLLQRGRDFSRFEDLDDRYDLRPS
                     AWVTPKGEWGKGSVELVEIPTNDETNDNIVAYWTPDQLPEPGKEMNFKYTITFSRDED
                     KLHAPDNAWVQQTRRSTGDVKQSNLIRQPDGTIAFVVDFTGAEMKKLPEDTPVTAQTS
                     IGDNGEIVESTVRYNPVTKGWRLVMRVKVKDAKKTTEMRAALVNADQTLSETWSYQLP
                     ANE"
     gene            10164..>10608
                     /locus_tag="ACHKBL_003619"
     CDS             10164..>10608
                     /locus_tag="ACHKBL_003619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFO5820436.1"
                     /translation="MNPVPAQREYFLDSIRAWLMLLGIPFHISLIYSSHTWHVNSAEP
                     SLWLTLFNDFIHSFRMQVFFVISGYFSYMLFLRYPLKKWWKVRVERVGIPMLTAIPLL
                     TLPQFIMLQYVKGKAESWPGLSLYDKYNTLAWELISHLWFLLVLVV"
BASE COUNT         2605 a         2772 c         2606 g         2625 t
ORIGIN      
        1 gcaattcatt ctccgacacg ccattttctt tctctcgcaa agtcgttttg gatgcactta
       61 tgtcattcac aaatacccct gaacgctatg gtgttatatc tgcggccttc cattggctaa
      121 gcgcaattat tgtctacggc atgtttgctt taggtctgtg gatggtcacg ctcagttatt
      181 acgatggctg gtatcaccag gcacccgaac tgcataaaag catcggtatt ttgttaatga
      241 tggggctggt tattcgcgtt ctgtggcgcg tcatatctcc accgccgggt ccgctgcaga
      301 gttattcgcc catgactcgt cttggcgcaa aggctggaca tctcgcattg tacttattgc
      361 tgttcgccat cggcatcagc ggctatctga tctcaactgc cgatggtaaa ccgatcagcg
      421 tttttggctg gtttgacgtc cccgcgaccc tcgccgacgc tggcgcacag gctgactttg
      481 ccggtgccct gcatttttgg ctcgcgtgga gcgtcgtagt actgtccgtt atgcacggat
      541 ttatggccct gaagcatcat ttcatcgata aagacgacac tctgaagcgc atgctgggaa
      601 agtcgtcatc tgactatgga gtataaaaaa tgaaaaaaag cctgcttggt ttaaccttcg
      661 cttccctgat gttctctgcc ggttcagcgg ttgccgccga ttacaaaatt gacaaagaag
      721 gtcagcacgc ctttgttaac ttccgcatac agcaccttgg ctatagctgg ttatacggca
      781 cctttaaaga tttcgacggt acttttacct ttgatgaaaa aaatccagct gccgataaag
      841 tgaatgtgac aattaacacc accagcgtcg atactaatca cgccgaacgc gataaacatc
      901 ttcgcagtgc agatttcctc aatactgcaa aatatccaca ggcaacattc acctccacca
      961 gcgtgaagaa agacggtgac gaactggata ttaccggcga tctgacgctg aatggcgtaa
     1021 ccaaacccgt cacgctggaa gcgaaattaa ttggtcaggg cgacgaccca tggggtggta
     1081 aacgtgcagg cttcgaggcc gaaggcaaaa ttaagctcaa ggacttcaat atcaagacag
     1141 atttaggtcc agcttctcag gaagtggatc tgattatttc agtggaaggc gtacagcaga
     1201 agtaagagtg aaatgttgcc ggatggcacg catccggcaa tgatatttat tctgttggat
     1261 caggaatgcc cagtgttgta ttcagacgtc cacgagactt attaaagatc ttattgccat
     1321 tttcacgtcc tgcccgacgg cgtcgctgtt cttctggcgg taacgcgctt tcttcgcagc
     1381 aaatttcact acaacaacct ttgtattttt ctgcacatac tggacactga ataaacagca
     1441 ggtggcagcc atcattttta cagttggtat ggctgtcgca cggcgcaccg cactggtggc
     1501 aatgcgcgat aatctcatca gatatacgtt cgcccatccg ctcgtcaaaa acaaaatttt
     1561 tgccaataaa acgtaccggt aaaccctgct cgcgcgcctt gcgggcgtat tcaataattc
     1621 cgccctcgat atgccagact ttattgaatc cgttatgttt catccaggca ctggcctttt
     1681 cacaacgaat gccgccggtg cagtacataa cgattttttt atctttatgt gcctgcatca
     1741 tctctactgc ttttggcagc tgctcacgga aggtatctgc cggaatttcc agcgcgtttt
     1801 caaagtgccc cacttcatac tcatagtggt tacgcatgtc gataaacaat gcatcgggat
     1861 cgtcaagcat ggcgttcact tccgccgctt gcagatactc acccacattg ctggcatcaa
     1921 agtgaggatc gtcaataccg tcggcaacga tacgatcgcg gaccttcatg cgcagtaccc
     1981 agaaggattt cccgtcatca tccaacgcga tattcaggcg taagccctct aaagccgggt
     2041 cgaaggcata aagctgcgcg cgaaatgttt caacattgct cgcaggtacg ctgatttgcg
     2101 cgttaatgcc ctcatgcgcc agatacactc gcccaaaaac atccagcgcg gtaaacagct
     2161 gatataaagc gtcacgggtc gccttaggat cggcgatgtg gaaatacttg taaaacgaaa
     2221 tggtggttcg cggttcgctc tcagccaaca ttttggcttt taacgcgtcg ttggaaatgc
     2281 ggttgtgtaa cactggcatg gtgtacggtt cctgcgagat gggaaagtaa aaatccgcgg
     2341 catgatatag caattatcga taattaacat ccacacattt tacgctacat ttgcgcatta
     2401 aaaattattt gttatttaca agcgcggcaa tttcgcccag tcttcagcca caaattttgg
     2461 ttgcgggcga aaaaatgcga caatacatac aattgcccgt ataggttgaa aaacaggatt
     2521 gatatgacga atctacccaa gttctccacc gcactgcttc atccgcgtta ttggttaacc
     2581 tggttgggta ttggcgtact ttggttagtc gtgcaattgc cctacccggt tatctaccgc
     2641 ctcggttgtg gattaggaaa acttgcgtta cgttttatga aacgacgcgc aaaaattgtg
     2701 catcgcaacc tggaactgtg cttcccggaa atgagcgaac aagaacgccg taaaatggtg
     2761 gtgaagaatt tcgaatccgt tggcatgggc ctgatggaaa ccggcatggc gtggttctgg
     2821 ccggaccgca gaatcgcccg ctggacggaa gtgatcggca tggaacacat tcgtgacgtg
     2881 caggcgcaaa aacgcggcat cctgttagtt ggcatccatt ttctgacact ggagcttggt
     2941 gcgcggcagt ttggtatgca ggaaccgggg attggcgttt accgcccgaa cgataatcca
     3001 ctgattgact ggctacaaac ctggggccgt ttgcgctcaa ataaatcgat gctcgaccgc
     3061 aaagatttaa aaggcatgat taaaaccctg aaaaaaggcg aagtggtctg gtacgcaccg
     3121 gatcatgatt acggcccgcg ctcaagcgtt tttgtcccgt tgtttgccgt tgagcaggct
     3181 gcgaccacga ccggaacctg gatgctggca cggatgtccg gcgcatgtct ggtgcccttc
     3241 gttccacgcc gtaagccaga tggcaaaggg tatcagttga ttatgctgcc gccagagtgt
     3301 tctccgccgc tggatgatgc cgaaactacc gccgcgtgga tgaacaaagt ggtcgaaaaa
     3361 tgcatcatga tggcaccaga gcagtatatg tggttacacc gtcgctttaa aacacgtccg
     3421 gaaggcgttc cttcacgcta ttaaatctcc caagccggat gcttgagaat agcatccggc
     3481 attaccacag caaatccccc tgatttagcg ataaaagctc tctggattgc gccccctgga
     3541 agtcgggcgc ataattagtg tgcttatctt ttcttcttat gttcaccgcg cctggcgcac
     3601 caacagcgga ttgctatgtc accctgtgaa aatgacaccc ctataaactg gaaacgaaac
     3661 ctgatcgtcg cctggctagg ctgttttctt acgggtgccg ccttcagcct ggtaatgccc
     3721 ttcttacccc tctacgttga gcagcttggc gttacgggcc actccgcctt gaatatgtgg
     3781 tccggtattg tcttcagcat tacattttta ttttcggcca tcgcctcacc gttttggggt
     3841 ggactcgccg accgtaaagg ccgaaaactc atgctattac gctctgccct cggcatgggc
     3901 atcgtgatgg tgttgatggg actggcacaa aatatctggc agtttttgat cctgcgggcg
     3961 cttcttgggt tacttggcgg atttgtcccc aacgctaatg ctcttatcgc cacacaagta
     4021 ccgcgtaata aaagcggctg ggcgctgggt acgctctcca caggcggcgt tagtggtgcg
     4081 ttgctcggcc caatggctgg cggcctgctc gccgatagct acggcttacg tccggtattc
     4141 tttattaccg ccagtgtgct catactctgc tttttcgtca ccctgttctg catcagagaa
     4201 aaattccagc cggtcagcaa aaaagagatg ctgcacatgc gggaagtggt gacatcactt
     4261 aaaaacccga aactggtact cagcctgttt gtcaccacgt taatcatcca ggtggcgaca
     4321 ggctcaattg cccccattct gacgctgtat gtccgcgaac tggcgggtaa cgtcagtaac
     4381 gtcgccttta tcagtggcat gatcgcctcg gtgccaggcg tggcggctct gctaagcgca
     4441 ccacgactcg gcaaacttgg cgatcgaatc ggacccgaaa agatcctgat tacagcgctg
     4501 atcttttctg tactgctgtt gatcccaatg tcttacgttc agacgccatt gcaacttggg
     4561 attttacgtt ttttgctcgg tgccgccgat ggtgcactac tccccgccgt acagacactg
     4621 ttggtttaca actcgagcaa ccagatcgcc gggcgtatct tcagctataa ccaatcgttt
     4681 cgtgatattg gcaacgttac cggaccattg atgggagcag cgatttcagc gaactacggt
     4741 ttcagagcgg tatttctcgt caccgctggc gtcgtgttat tcaacgcagt ctattcatgg
     4801 aacagtctac gtcgtcgtcg aataccccag gtatcgaact gatttttcgc ctttcatact
     4861 tgcaaaagcg gagaatcagc tatccttttc cctgaaacct catcaactca aagggagaat
     4921 cgtgatgacc atgtacgcaa cgcttgaaga agccattgac gctgcacgcg aagaatttct
     4981 tgcagacaac cccggcatcg acgccgaaga tgcgaatgtg caacagttca atgcccaaaa
     5041 atacgttttg caggacggcg acatcatgtg gcaagttgag ttttttgccg acgaagggga
     5101 agaaggtgaa tgtttaccta tgcttagcgg tgaagccgcg caaagtgttt ttgatggcga
     5161 ctatgatgag atagagatac gccaggagtg gcaggaagag aatacattac atgaatggga
     5221 cgagggggaa tttcagcttg agccaccgct ggataccgag gaaggacgcg cagcagctga
     5281 tgagtgggat gaacgttaat cactcatacg ggccatgatg aatgtcgatc ggcagcagta
     5341 aagtatcgaa aaccagggag aatggcagat caaggatcgt gacgtaacgc caggcggagt
     5401 cacgcacgtc ccattgcaca ccgggataat attggttgcc atgcccctgc cccggtatag
     5461 tgcgactaat gatgctgcca cagccactca gcaaggtcac cattatgctc atcacaatta
     5521 aacgcatttg atacctcttc cgtaaaaaat accggcataa cgccggtatt tttattggct
     5581 cgttgttttt gccggatgcg gcgtgaacgc tttacccgac ctacaaaatc ttgcaaaatc
     5641 aataaattgc aggaacgatg taggcctgat aagcgtagcg catcaggcaa ctacgttttt
     5701 attgcgaagc cgcatccggt ttacgcaatg ccagtggatt gagctttatc ccttcgtaat
     5761 aactcaccca tgaagaatat ctctccgggg aattccagac gcggaaatgc agacgggcca
     5821 tcgtcaccgg atcgcttagc agcaccaggc gacgatcgcg attcagcttc tctggcgtct
     5881 cgttcagcgc ctgttcaacg tggcggtcac gggcgatttc cagcaccttg ctggcgcggt
     5941 gacgcgcggt cgccattgcg gttgccagag cgttaaatga cgggttaaac accgcgtgca
     6001 taaaaccatc atcaagggag cgttgacgat tcatctcaag gaaccgatcg gtatcaacca
     6061 gcacctgcgg cggtgaatac tcttccggga tcaggaacag tttccagcgt ttggttcgca
     6121 gaccaacggt ggcacggctg gaaatcaccg aaacaaacgg tgacaggatc aacgagaaga
     6181 caatcggtgc cagccagaac agaaaacgca gatccaacca cgccatccca acagcccaca
     6241 ctaaacccaa cagcagctgt gagccgtggc gtttgaatgc ttcgccccag gaagtggagt
     6301 catcatcacg ctgcggtgaa ttccacacca cttcccagcc aaggaacgcg ctgacgacga
     6361 agactgtatg gaacaacatg cgtaccggag ccagcaacac ggaaaacagc acttccagca
     6421 gcaacgataa tgtaacgcgc cagaagccgc cgtattcttt cgttcctttg caccagataa
     6481 gcaaaatgct caatagcttc ggcaggaaca acagcaccat ggtcgaagca aaaagtgcaa
     6541 tcgccagctc aggacgccac tgcggccata ccgggaacaa ctgccgtggt tgcaggaagt
     6601 attgcggttc ggtcaacgca tgtactacct gcaatgcagt agagagcgcg aggaacataa
     6661 accacagcgg agcggagaga taagacatca cgcccgtcag gaacaccgca cggtgaaccg
     6721 ggtgcatacc cttcaccagg aacagacgga agttcatcag gttaccgtgg caccagcggc
     6781 ggtcacgttt tagctcatca agcaagttag gcggcaattc ttcataagaa cccgggagat
     6841 cgtaagcaat ccacaccccc caacctgcac ggcgcatcaa cgccgcttct acgaagtcat
     6901 gtgacaggat tgaaccggca aaagaacctt cgcccggcag cggagccagt gcacaatgct
     6961 cgataaacgg tttcacgcgg ataatcgcgt tatgccccca gtagtgcgac tcgccaagtt
     7021 gccagaagtg caaaccggct gtaaacagtg gcccatacac gcgggtcgcg aactgctgac
     7081 agcgcgcata cagcgtatcc ataccggacg ctttcggcga cgactgaatg atcccggcgt
     7141 tcgggttggc ttccatcaag cgcaccaggc cgcataaaca atcaccggtc attaccgagt
     7201 cagcatccag caccaccatg tagctgtact ggctgcccca gcgacggcag aagtcatcga
     7261 tattaccgct tttacgcttc acgcgacggc ggcggcggcg atagaaaatc tgaccttcgc
     7321 caccgacttc agcgataagc tccatccagg ctttttgctc tgcgacgcag atatccgggt
     7381 tataactgtc actcagaatg tagacatcaa agtgtttggc attcccggtg gcttttactg
     7441 attcccacgt tgcacgcaag ccagcaaaaa cacggttcac gtcttcgtta cagataggca
     7501 tgatcaacgc cgtgcgatgc tccgggttta atggttcatc gccaactgtt gacgcggata
     7561 tactgtattt atcgcgacca ataagcagtt gcaggaagcc catcaacgcc gtccagaatc
     7621 cggcggacac ccaacagaac agtaccgcaa agaggatcag gataccggtt tgcagcatat
     7681 aaggcagaag ctgcataaaa gaaacccaca catcctgacc aaccatatcc ataggattaa
     7741 tcagcgccca cccctgataa ggaagaatgg tcttcatata ccaggtcgcg acaacagttt
     7801 gcgcgagcgt caggatcaac agaatgtaac ggcggatggt accgacggta cgccactttt
     7861 gctcgctctc ctgctcttct ttggtcaaac gagccagata gcgcggcgtc acatcgcgtc
     7921 cacgcaggcg atcccagaaa cggcctaccg ggttggtacg ccacgggtcg ggaaacatcg
     7981 aggagcgttt tgcttctggc atcgccttaa gctgatcgcg cccttcgtca tctttaatta
     8041 actgtccatc agcaagtgaa tctggccagg cttgttccag acgcgccttt accgagcctt
     8101 gcggggagtc atcctcccgc gcccaggtgc ggtgttcggc atccagcgcc tgatgaacgg
     8161 cgcggatatc agtcttcggc aatgccgctt tctcgcttgc ggcgatgggc attgcgtcaa
     8221 tgtactcagt tgtcttattc attggcaggt aactggtagc tccaggtttc actcaacgtc
     8281 tgatcggcat tcaccagcgc agcacgcatt tcagtggttt tcttggcatc tttcactttc
     8341 acacgcatca ccagacgcca gcctttggta accgggttat agcgcaccgt gctttcaact
     8401 atctcaccat tatcaccaat gctggtttgc gctgtgaccg gggtatcctc tggcagtttt
     8461 ttcatctctg cgccggtaaa atcgaccaca aaggcgatag taccgtcagg ttggcgaatc
     8521 aggttcgact gcttcacatc ccccgttgaa cgacgcgttt gttgcaccca tgcgttatct
     8581 ggcgcatgca gtttgtcttc atcacggctg aaggtgatgg tgtatttaaa gttcatctct
     8641 ttacccggct ccggcagctg atccggcgtc cagtaagcga cgatgttatc gttggtttca
     8701 tcgttggttg gaatttccac cagctcaacg ctgcctttgc cccactcccc tttcggagtc
     8761 acccatgcgc ttggacggag atcgtaacga tcatcgagat cttcaaagcg ggagaaatca
     8821 cgaccgcgct gcaacagacc gaagccttgc gggttttcca tcgagaagct gctgaccgct
     8881 aaatgtttcg ggttattcaa cggacgccag atccattcgc cattaccagc atggatagag
     8941 agaccgttag agtcgtgcaa ctccggacga tagttatttg caggcgacgg ttggttcggc
     9001 ccaaacagga acatactggt taacggtgca acccccagtt tgccgacttt atcgcgcaga
     9061 tagattttcg actgcacatc cacaaccgtg tcacgccctg gcataactac gaatttgtaa
     9121 gcacctgtcg cgcgcggcga gtcaagcaat gcataaatgg ttaaacgttt atcagtcggt
     9181 tttggacgct cgatccagaa ctctttgaag cgtggaaatt cttcacccga tggcaaggcg
     9241 gtatcaattg ccaggccgcg tgcagaaagg ccataaacct gacctgcacc aatcacgcgg
     9301 aaatagctgg ccccgagcat gctgacgatt tcatcgtttt tatctttgct gttgatcggg
     9361 taaagcactt taaaaccggc aaaaccaagg tcttttaccg tgtctttgtc atgctgaaca
     9421 tcgccgaaag tgaaataatc cgggctgtat ttgattcgtt tgactgcggt ggcagtcact
     9481 tcatttattt tgaccggggt atcgaagtac ataccctgat ggtagaactc gagtttgaat
     9541 ggggtcttca gattgttcca gtacgctttg tcatgattaa actggatctg ctgatagtcc
     9601 gcgtatttca tgtcgcggaa aacggagggc aagttgcttt taggcgcctc atagcctttc
     9661 ccggctaagg attgagcttg ctttgcgaca tcatcaatac tgaaagccca gcttgaagat
     9721 gtatacaggg ttaacattac tgcagcactc aaccaacgca ttttcatcat ttgtagttta
     9781 tgtttcataa ttagtaagca cttccccctt tgtgtgctta tatcgatccg atctatttta
     9841 atggataacc agccattccg acaacagaat cccggtatcg ttcagcacgc tggctcatgg
     9901 attaagcacc tgattgcaac cgttttcact ttccgtgctt ctcctaaaga cagaagcatc
     9961 gacttcgtgt agggtttgcc agcaaattta tttatcaacc gtcttacaga cgcttccatt
    10021 ctggttagtg gtagcgatga tgctatgcat aaaaaccttg aagcctgcgc ctggcgcggt
    10081 caggtcggtg tcgctatggc aacaagccta aaggcataac ccgacaggga taagacgaaa
    10141 aatccgagat tagttaacca tatatgaatc cagtacccgc gcaacgtgaa tatttcctcg
    10201 actccatccg cgcctggctg atgttgttag ggatcccttt tcatatttct ttaatctatt
    10261 cgagccatac atggcatgtg aatagcgccg aaccgtcatt gtggctgacc ctttttaatg
    10321 acttcatcca ctcgttccgc atgcaggtat ttttcgttat atccgggtac ttttcctaca
    10381 tgcttttttt acgctatccc ttgaaaaaat ggtggaaagt acgtgtcgaa cgtgtaggta
    10441 tcccgatgtt aacagccatc cccctactga cattaccgca atttattatg ctgcaatacg
    10501 tcaaagggaa agcggaaagt tggcctgggc tatcattgta tgacaaatat aatacgttgg
    10561 cctgggaatt aatatcacac ctgtggtttt tactggtgtt agtagtca
//
LOCUS       DAZFLD010000161        10602 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.162, whole genome shotgun sequence.
ACCESSION   DAZFLD010000161 DAZFLD010000000
VERSION     DAZFLD010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10602
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.162"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(100..777)
                     /locus_tag="ACHKBL_003620"
     CDS             complement(100..777)
                     /locus_tag="ACHKBL_003620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000208494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HFO5820437.1"
                     /translation="MTTQVSVETLSTITYKQIPVITTELLAHLYGTEAIRIRQNHHEN
                     KGRFIEEKHFFKLEGETLREFKHRVAFNYSVKIARNVRSLILWTERGAARHAKMLETD
                     RAWEVFEKLEDCYFSQKIPEQLQLPESTLSINYPLSWFSEHHPYSMMSYVDRKTLNLD
                     VSVLFDMPSPTMRILNELHSKGYNVDAAVAEFNAFKHLTEEMRRTLQDISRLSDRNSR
                     KGFSLSL"
     gene            1102..1359
                     /locus_tag="ACHKBL_003621"
     CDS             1102..1359
                     /locus_tag="ACHKBL_003621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051983 - regulation of chromosome
                     segregation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HFO5820438.1"
                     /translation="MRTTQQFSITLTNEMADMVRARVASGAYASESEVIREGLRALNE
                     RDKAMEAWLIHSAAPPLDAIRENLGKGHSISQVRSEIRARK"
     gene            1413..1685
                     /locus_tag="ACHKBL_003622"
     CDS             1413..1685
                     /locus_tag="ACHKBL_003622"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5820439.1"
                     /translation="MSLAAPIRDPIMNIIARFFYLIIFCMATSGCTTTTKINRGDEKE
                     QYIIACGAATPWGLCYDKANALCKNGYKDILKEQGFNRKELTIECK"
     gene            1775..2080
                     /locus_tag="ACHKBL_003623"
     CDS             1775..2080
                     /locus_tag="ACHKBL_003623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001240641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HFO5820440.1"
                     /translation="MAMFNPPHPGEIIADILEDQNIGIRELARALDVAPSTVQRLVSG
                     NTTISPEMAVRLAAVLGGTPSSWIRLQTAWSLEKAEREVDVSHLSTKYRPAEISPHA"
     gene            complement(2123..2860)
                     /locus_tag="ACHKBL_003624"
     CDS             complement(2123..2860)
                     /locus_tag="ACHKBL_003624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000795072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5820441.1"
                     /translation="MKNNVMKNGTIDSQALLKMVNEARRLCGEKEVRNNDFIARIKDE
                     LDGEGYEIFVTPMDKKKGGADQVVIVMTYKQALRVAARESKSVRRSLVDQLESMQQQL
                     QQKITSKHSTNGLEEFRKARALKMTVDTMKDLFGFLPNLAPEAKQVVAASLVNPVVGA
                     NVIPLPMINEHYYSASEVGAKLKISANKVGRIANTYMLKTEQYGKWFIDKSPYSDKQV
                     ESFRYNNRGVRKIEEILDAENKAEFGT"
     gene            complement(2956..3516)
                     /locus_tag="ACHKBL_003625"
     CDS             complement(2956..3516)
                     /locus_tag="ACHKBL_003625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016241157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HFO5820442.1"
                     /translation="MIEGISNKSLNTACVDGGLLYKVTFTQIDNKDNAFTVIYTSPEM
                     AKKWVDLHRLCDFRVEWGCYEMRCRWHHRGDEKMSTTLEQWLEQQHGKIDVDCGCVST
                     ETLMHWMRVAYEAGNSPVTPDGWISCSERMPDDGQWCVVNTEYGYYVQCWSEGQGWLG
                     DYISIPECDVINWMPLPEPPQEVNRG"
     gene            complement(3513..3878)
                     /locus_tag="ACHKBL_003626"
     CDS             complement(3513..3878)
                     /locus_tag="ACHKBL_003626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HFO5820443.1"
                     /translation="MSKIDYQALRDAAVAIETVATPQKLLAFRMKVTPSVVLALLDEI
                     KRLEDTNIDAMCRIAELEAREAELPDGYEPRMGHPINSGERVVMMPHHFGGWLDRFDV
                     EHALQAAGIKFKTAGGEYE"
     gene            complement(3875..4549)
                     /locus_tag="ACHKBL_003627"
     CDS             complement(3875..4549)
                     /locus_tag="ACHKBL_003627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HFO5820444.1"
                     /translation="MNKIDYQVLRDVSERAITAMTHLSILPGDDDLLSEKKLKELGID
                     IDAIHAFKIMAGPETVLSLLDERDALNERIAELEANLAKLAEDQQKAIESIKQADSAV
                     KLAHEKFSALAAENARLKAAHPQPFGHEMMKALDAYEKHQDEVPETGMLNAFFILRDS
                     ICVETPATDSFLSEVRAQAFNDLCSVFVKDATVVGLDDGDIVTVKEAKDALLHCAEQL
                     RKGGNQ"
     gene            complement(4546..4785)
                     /locus_tag="ACHKBL_003628"
     CDS             complement(4546..4785)
                     /locus_tag="ACHKBL_003628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000476205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5820445.1"
                     /translation="MFKQNEKSIAQIAEYIPRTCRGMQLQEAKARLEKKIALYIDDGC
                     DAAVLNAAFAPALNCHTRESFFSCIAAQIHKGGNQ"
     gene            complement(4778..4981)
                     /locus_tag="ACHKBL_003629"
     CDS             complement(4778..4981)
                     /locus_tag="ACHKBL_003629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019705292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5820446.1"
                     /translation="MTIVKTHTGIVITKDGPQVKKLHQTERMWVVGKNEFYHKETGRR
                     HFAENTRRRLLIDTIKPVEVKHV"
     gene            complement(4978..5856)
                     /locus_tag="ACHKBL_003630"
     CDS             complement(4978..5856)
                     /locus_tag="ACHKBL_003630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000335005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase family
                     protein"
                     /protein_id="HFO5820447.1"
                     /translation="MSTHHVVSFSGGRTSAYLVHLMEQRRKAGENVHYILMDTGCEHP
                     LTYRFIREVVKFWDIPLTVLQVDINPELGQPNGYTEWEPKDIQTRMPVLKPFMDMVKK
                     YGTPYIGGAFCTDRLKLTPFTKYCDDHFGQGNYITWLGIRADEPRRLKPKPGVRYLAE
                     LSDFDKSDVIRWWRKQPFDLQIPEHLGNCVFCIKKSTQKLGLACKDEPGLMRVFNELV
                     TGKHVRDGHRRTGKDVMYRGHLTLDGIARMYADSDYSNLYQAMVLAKRFDTGSCSESC
                     EIWGDQLELKFEEVVA"
     gene            complement(5964..6407)
                     /locus_tag="ACHKBL_003631"
     CDS             complement(5964..6407)
                     /locus_tag="ACHKBL_003631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5820448.1"
                     /translation="MQFKDLPEDIQKIAADTLKAHLSVLDLTKEPKANLENISRNVRN
                     VFVGLYAYDNEKHEEHIQNGCINKCQQNIKLPVADKTEQKTDVLVFWNTVEAIARSTM
                     LEFNNINHSGIDTHQSSHREGFSILLTLLQEQGEYPQPTSNDTEQ"
     gene            complement(6484..7305)
                     /locus_tag="ACHKBL_003632"
     CDS             complement(6484..7305)
                     /locus_tag="ACHKBL_003632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdQ family protein"
                     /protein_id="HFO5820449.1"
                     /translation="MSQLDSNAIKEIVKLTTTAFSGENLPLTECPVALLPDNVNIESL
                     ERFMTERFRFRGVMTTTSIDDFVEYSKGYADEHSRCFINAETMKAVTVFNIGTLEQPG
                     HADNKALLELKATSPYRALRDVDGKRQLQKSLAEWLEDWADFLTAYDSDGNVLDIKQA
                     ISAIRRLTIDAKRSAEYEEQNFSGSRSVMESVEAKSKEIMPATFRFECIPYEGLGNRE
                     FTLRLSILTSEQPVLVLRIVRVEAAEEEIAKEFRDLLKERFEEEDILTFIGTFSV"
     gene            complement(7369..7716)
                     /locus_tag="ACHKBL_003633"
     CDS             complement(7369..7716)
                     /locus_tag="ACHKBL_003633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001071603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5820450.1"
                     /translation="MNQHQTDVNVFINDLDGGVFVNKLGAVLSEVAFGVNSTNKKGKV
                     CVEFELSSLDENRVSVSHKLKFTRPTMRGSKSEEDTTNTPMFVNKGGELTLFQKDQGQ
                     LFDKQGQHDAVLR"
     gene            complement(7791..8378)
                     /locus_tag="ACHKBL_003634"
     CDS             complement(7791..8378)
                     /locus_tag="ACHKBL_003634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF669 domain-containing protein"
                     /protein_id="HFO5820451.1"
                     /translation="MDEVIFTYNEESALTAGQGGFITETGAHIINITEAELKQSEKGA
                     RFIEFSGESDDGRKIQYLSVCVQKNDGTENKFGASIIHAMMGCTGIGQLTQHMVSVSK
                     YVAPEFHGKKIGLVLQKVLTTNRKTGADGYQMEIRIPFIAETGQTLKEKAEGKKPETV
                     ANMVSTLKDKDNRRKNVNPNHTDDPGYWQYGSDSF"
     gene            complement(8378..9067)
                     /locus_tag="ACHKBL_003635"
     CDS             complement(8378..9067)
                     /locus_tag="ACHKBL_003635"
                     /EC_number="3.1.11.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000187064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lambda exonuclease family protein"
                     /protein_id="HFO5820452.1"
                     /translation="MTPEIILQRTGVDITSLDQGDDGWHKLRLGVITASEVHNVIAKP
                     RSGSKWPDTKMSYFHTLLAEVCTGVAPEVNAKSLAWGKQYEDDARALFEFIADVTVSE
                     TPIIFRDESMRTACSPDGLCSDGNGLELKCPFTSRDFMKFRLGGFDAIKPAYMAQVQF
                     SMWVTDKDAWYFANYDPRMKREGLHYVVVERDEKYMASFDEMVPEFIDKMDEALAEIG
                     FVFGEQWGVNN"
     gene            complement(9064..10014)
                     /locus_tag="ACHKBL_003636"
     CDS             complement(9064..10014)
                     /locus_tag="ACHKBL_003636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000459722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HFO5820453.1"
                     /translation="MFDIVEFVKQQERFFCEALTEPTLTWAKESQFAIQQFQKNAFLA
                     DTARANLPSAQNAIINVAAIGITLNPASKLAYLVPRKKAVCLDISYMGLLHLAQVTGA
                     IQWGQCKLVYEKDIYESNGIDCAPTHKYNPFVDRGARIGGYCVVKTSEGDYLTEEMSN
                     REIEVIRACSKAGNNGGSSPWDSFPDEMARKAIVKRASKYWPRRDRLDTAIDYLSTQA
                     GEGIILNADHIPERDVTPASDEIINEITQAITEINKTWDDLLPLCSKTFRRTIASHEY
                     LSQEEAVKTLDFVKKKAARNKATAEAKIHATTENNSEAVS"
     gene            complement(10032..10313)
                     /gene="gamL"
                     /locus_tag="ACHKBL_003637"
     CDS             complement(10032..10313)
                     /gene="gamL"
                     /locus_tag="ACHKBL_003637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000995345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host nuclease inhibitor GamL"
                     /protein_id="HFO5820454.1"
                     /translation="MNAYLTCDRIEERRWINQQIQDEKDKWIDDRAQELISMFPDKPL
                     LMSSLFLPKEAQLALTGEKAEEAYNDYISAIAYARAEEEWERKFSPCPF"
     gene            complement(10334..10600)
                     /locus_tag="ACHKBL_003638"
     CDS             complement(10334..10600)
                     /locus_tag="ACHKBL_003638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000934192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFO5820455.1"
                     /translation="MNINVKSVVTPAGTIMGEVFMDDKIIAYFVILSDEAISVIDTEG
                     NVMFIAEHPEDIALQAAAYFFAKEQEEECNCPVCQLSRQINLMH"
BASE COUNT         2512 a         2607 c         2429 g         3054 t
ORIGIN      
        1 gcaccgggcc ttccagaacg cctgtagaaa gcgatctgtt tcaaattttt gtctccagat
       61 atgaaaaccc cgacgggatg tcggggatgg taggggaagt tacaggctta acgagaagcc
      121 ttttcgggaa tttcgatctg acaatcttga aatatcctgt agagtacggc gcatttcttc
      181 cgtcagatgc ttgaaggcgt taaattcggc gacagcggcg tcaacgttat agcctttgct
      241 gtgtagctcg ttgaggatgc gcatagttgg gcttggcatg tcgaagagta cagaaacgtc
      301 caggttaaga gtttttcgat caacatagct catcatggag taggggtggt gctctgaaaa
      361 ccacgagaga gggtagttga tagatagcgt tgattctgga agctgaagtt gttctgggat
      421 tttttgactg aaatagcagt cttccagttt ttcgaacact tcccacgccc gatcggtttc
      481 gagcattttt gcgtgacggg ctgcgccgcg ttctgtccag aggatgaggg agcgaacgtt
      541 acgggcgatt ttcacagagt agttaaaagc tactctgtgc ttgaactcac gtaaagtttc
      601 accttcaagt ttgaagaagt gtttttcctc aatgaaacga cctttgtttt cgtggtgatt
      661 ctggcgaata cgaatagctt ctgtgccgta aaggtgcgcc aaaagttcgg tagtgataac
      721 ggggatctgc ttgtaagtaa tcgtggagag tgtttcaaca gaaacttgag ttgtcattat
      781 gacgccctcg agtggtttct aaactatcac caccgttagg ttcgaatcat cgggtggtga
      841 gacgtacagg gttcgaacta ccgggaaacc aaccggcgag cttttcagct cccctatacg
      901 ccccaccata attcagatgt gcgcgtgcat acgacaataa aaaacacgct cgcggcgtgt
      961 ctctgtcgcg gtttctatcc ggggttcgaa tcccgacggt caactcgacc gtgcgaagaa
     1021 tataatcccg gatatgtgtt gtcgtcaaca agtggcgtgc tatcatcgaa tagtgttcta
     1081 tcctactcag tgaggtttaa catgcgtaca acccaacaat tcagcattac attaaccaac
     1141 gaaatggctg acatggtgcg cgcccgtgtg gcttccggtg catacgcttc agaaagcgaa
     1201 gttattcgtg aagggcttcg tgcactgaat gaacgcgaca aagcaatgga agcgtggtta
     1261 atacactcag ccgcgccgcc actcgacgct attcgtgaga acctaggcaa agggcactcc
     1321 atttcacagg ttcgctctga gattcgcgcc cgaaagtaat ctgcatggca tatgaagtgc
     1381 cacactaaca aactgctcca ggtatgggaa tagtgtcact tgcagcacct attagagatc
     1441 cgataatgaa catcatagca aggttctttt atttgataat tttctgcatg gcaacatcag
     1501 gatgcactac cactacgaaa ataaatcgcg gagatgaaaa ggaacagtac atcattgcct
     1561 gtggtgccgc tacaccatgg ggactatgct atgataaagc caatgcccta tgtaaaaatg
     1621 gatataaaga cattttaaag gaacagggat ttaacaggaa agaattaacc attgagtgta
     1681 aataaggcca tcatttgcaa cgttgaaaaa attcgcccgt gctacaggga aaaaactcca
     1741 gagccgcttc gtttaactac gaggattacc actaatggcg atgtttaacc cccctcatcc
     1801 cggagaaatt attgctgata ttctggaaga tcagaatatt ggaatcaggg aactggcaag
     1861 agcgcttgat gtcgcgcctt ccactgttca gcgactggtt tcaggtaata cgacaatatc
     1921 tcctgaaatg gctgttcgtc tcgctgctgt cctgggtgga actccgtctt catggattcg
     1981 tctccagacg gcatggagcc ttgaaaaagc ggaaagagaa gttgacgtat ctcatctctc
     2041 aacaaaatac cgcccggcag aaatttcgcc tcatgcttaa ccaccgcgcc gtcattctgg
     2101 cggcgtcgga gagtggagga tatcaggttc cgaactctgc tttgttctcc gcgtccagga
     2161 tttcttcaat ctttcgtact ccccgattat tgtatcgaaa gctttctacc tgcttatccg
     2221 aatacgggga tttgtcgatg aaccatttcc cgtattgttc tgttttgagc atgtaggtat
     2281 tggcaatgcg accaaccttg ttagcggata ttttgagttt tgctccaact tctgacgccg
     2341 aatagtaatg ttcattgatc atcggtagcg ggattacgtt agcgcctaca acgggattaa
     2401 ccaggctggc ggcaacgacc tgtttagctt caggggcaag gttggggagg aagccgaaaa
     2461 ggtctttcat tgtatcgacg gtcattttca gcgcccgtgc tttacggaat tcctcaaggc
     2521 cgttagtcga atgtttcgag gtgatttttt gttgcagttg ctgttgcatt gactccagtt
     2581 gatcgaccag cgaacgacgg acggatttag attcacgagc ggcaacccga agcgcttgtt
     2641 tataggtcat cacaataaca acctgatccg caccgccttt tttcttatcc atgggggtta
     2701 cgaaaatttc gtaaccctcc ccatcaagtt catctttgat gcgtgcaatg aagtcgttgt
     2761 tgcgtacttc tttttcaccg cacagtctcc gcgcttcatt caccattttc aacagagcct
     2821 ggctgtcgat agttccattt ttcattacat tatttttcat cgttttaacc tcttaagctc
     2881 gccgtagcga gttcagataa aagaaatccc cgcgagtgcg aggattctta ttcacctttg
     2941 acggcaagtt gcaggttagc cacggttaac ctcctgcggc ggttctggta gcggcatcca
     3001 attgattaca tcgcattcag ggatgctgat ataatcacca agccatcctt gaccttcaga
     3061 ccagcattgc acgtaatacc cgtattctgt gttcactacg caccactgcc cgtcatccgg
     3121 cattcgctca ctacagctta tccaaccatc cggagttacc ggagagttgc cagcctcata
     3181 cgccacacgc atccagtgca taagcgtttc agtgctcaca cagccgcaat caacatcaat
     3241 tttgccgtgc tgctgttcca gccattgctc aagcgtagtg ctcatttttt catccccccg
     3301 atgatgccat ctgcatcgca tttcatagca cccccactca acacgaaaat cacatagcct
     3361 gtgcaggtcc acccacttct tcgccatctc tggcgacgtg taaatcacag tgaatgcgtt
     3421 atctttgttg tcgatttgcg taaacgtcac tttgtacaac aacccaccgt cgacacatgc
     3481 tgtgttcaac gatttattgc taataccctc aatcattcgt actcaccccc cgctgttttg
     3541 aatttgatcc ccgctgcctg caatgcgtgc tcgacgtcga agcggtccag ccatccacca
     3601 aagtggtgcg gcatcattac cacccgctca ccgctattta ttgggtggcc catacggggt
     3661 tcgtagccat ccggtaactc ggcttccctc gcttccagtt ctgcaattcg gcacatagca
     3721 tcaatatttg tgtcctccag gcgcttaatt tcatccagca gcgccagtac caccgacggt
     3781 gtgactttca tacgaaatgc cagcaatttt tgaggcgttg ctactgtttc aattgctact
     3841 gctgcatcac gcagtgcctg atagtcaatc ttgctcactg gttgcctcct ttgcgaagct
     3901 gttccgcaca atgcagcagg gcgtccttcg cttctttcac cgtaacgatg tcgccatcgt
     3961 ccagcccgac aaccgtcgcg tccttaacga acaccgagca aaggtcatta aacgcctgcg
     4021 cccgcacttc agacaggaaa gagtcggtgg ctggggtttc aacacagatg ctgtcgcgca
     4081 agatgaaaaa tgcattgagc attccagtct ctggcacttc atcctgatgc ttctcatacg
     4141 catcaagagc cttcatcatc tcatgtccga atggttgagg gtgcgcagcc ttcagcctgg
     4201 cattctccgc cgccagcgct gaaaacttct cgtgtgccaa cttaacagcc gaatcagcct
     4261 gcttaattga ctcaatcgct ttctgttggt cttcggccag cttcgctaaa ttagcctcca
     4321 gttcggctat gcgttcgttt aatgcatctc gttcatccag tagtgacagc acggtttctg
     4381 gcccggccat aattttaaag gcgtgaatcg catcaatatc aataccaagt tccttgagct
     4441 tcttttcgct taataaatca tcatcacctg gtaatattga cagatgtgtc atcgctgtaa
     4501 ttgcgcgttc cgacacgtca cgcaatacct gatagtcaat tttgttcact ggttgcctcc
     4561 tttgtggatc tgcgctgcga tgcacgaaaa aaaagattcc cgcgtatgac agttaagagc
     4621 tggtgcgaaa gccgcgttaa gaacggcagc atcacagccg tcatcgatat agagcgcaat
     4681 ttttttctcc aggcgcgctt tggcttcctg caactgcata ccccggcacg tacgcgggat
     4741 atactcagca atttgagcga tagatttttc gttctgttta aacatgcttc acctcgacag
     4801 gcttgattgt gtcgatcagc agtctgcggc gcgtattttc tgcaaagtgg cggcgtccgg
     4861 tttctttgtg gtaaaactcg tttttgccga cgacccacat ccgctctgtc tggtgaagtt
     4921 tttttacctg cgggccgtct ttggtgatca caatgccggt atgggttttt acgattgtca
     4981 tgccactacc tcttcgaatt tcaactccaa ttggtcaccc cagatttcac atgattcgga
     5041 acacgaacca gtatcaaacc gcttggccag caccatcgct tgatacaaat tgctgtagtc
     5101 gctgtcggca tacatcctgg caatcccgtc aagcgtcagg tgaccacggt acataacgtc
     5161 tttacctgtt ctgcgatgac catccctgac gtgtttgcct gtaaccagtt cattaaaaac
     5221 ccgcatcaga ccaggttcgt ctttacatgc aagccccagc ttttgcgttg actttttgat
     5281 gcagaaaaca cagttcccga ggtgctccgg gatttgcaaa tcaaaaggtt gttttcgcca
     5341 ccaccggata acatccgact tatcaaaatc tgacagttcg gcaagatacc ggacgcccgg
     5401 tttcggtttc aacctacggg gttcgtctgc acgaataccc agccacgtga tgtagttccc
     5461 ttgtccgaaa tggtcatcgc agtattttgt gaagggggtg agttttaatc tgtcagtgca
     5521 gaacgcgccg ccgatgtatg gcgtgccata tttttttacc atgtccataa acggtttaag
     5581 caccggcatt cgcgtctgaa tatcctttgg ttcccattcc gtataaccat ttggctgccc
     5641 aagctctgga tttatatcga cctgcaacac ggttagcgga atatcccaga acttcacaac
     5701 ctcccgaata aaccggtacg tcagcgggtg ttcacatccc gtatccatga gtatgtaatg
     5761 aacgttttca ccagcttttc tgcgttgctc cataaggtgg acgaggtacg ccgatgtccg
     5821 accgcctgaa aaactcacaa catgatgagt gctcatttct tcgtctcgtt acggggaagg
     5881 ggttaaatag gtggtaagct ggaattgccg gatgtttacc cgtgtccggg gtacgacctc
     5941 acgttgcagc gtacgagaat ggactattgc tcagtgtcgt ttgatgtcgg ctgaggatat
     6001 tcgccttgct cttgtaacag agttagaaga atggaaaacc cctcacgatg tgatgattga
     6061 tgagtgtcga tcccggaatg attgatgttg ttaaactcca acatcgtgct cctggcgata
     6121 gcctcaacgg tattccagaa tactaataca tctgttttct gctctgtttt atcagcaaca
     6181 ggaagtttga tattttgttg acatttatta atgcaaccat tttgaatatg ttcctcgtgc
     6241 ttttcattgt cataagcata cagcccgaca aaaacatttc gcacgttacg ggatatattt
     6301 tccagattcg cctttggttc ctttgttaaa tcaagcaccg ataaatgggc tttaagcgta
     6361 tccgcagcta ttttctgaat atcctctggt aaatctttaa attgcatagt ataccctcca
     6421 ggaactgtgt attttctcgt gaaatatgag cgatatgtcg tgacgtccct gtcacagatt
     6481 tcatcacact gagaacgtgc cgatgaaggt taagatatct tcttcttcga aacgctcttt
     6541 cagcaggtca cggaactctt tggcaatttc ttcttccgca gcttcaacac gcacgatacg
     6601 tagcaccaga acaggttgtt cgcttgtcag aatgcttaat cgcagcgtga attccctgtt
     6661 gcccaggccc tcgtatggga tgcattcaaa gcggaaagtg gcaggcatga tttctttgct
     6721 tttagcctcc acggactcca tgactgagcg gctaccgctg aagttttgct cttcatattc
     6781 agcactgcgt tttgcgtcga tggtaagacg acggatagca gagatagcct gtttgatatc
     6841 cagcacatta ccgtcgctgt cataggctgt gaggaagtcg gcccagtctt ccagccattc
     6901 agcgagagat ttctggagtt gtcttttgcc gtccacgtca cgtaatgcgc gatatggtga
     6961 tgtggctttc agttccagca gtgccttgtt gtcagcgtgt ccaggttgct ccagagtgcc
     7021 gatattgaac acagtgactg ctttcatcgt ttccgcgtta atgaagcagc gggaatgctc
     7081 atcggcatac cctttgctgt attcaacaaa gtcatcaata ctggttgtgg tcatgactcc
     7141 acggaaacga aaacgttctg tcatgaatcg ttccagactt tcgatattta cattgtccgg
     7201 caataacgca acagggcatt ccgtaagcgg caaattttca ccagaaaaag cagtagtggt
     7261 gagttttaca atttctttaa tggcattgct atctaactga gacatgtata tttaccttta
     7321 atttatggat ttagggataa tgcgcgagcg ttttaaggcg gggattattc agcgtaaaac
     7381 agcgtcatgt tgaccctgtt tatcgaaaag ctgtccctgg tctttctgga acaaagtaag
     7441 ctcaccacct ttatttacaa acatcggggt gttagttgtg tcttcttctg atttactacc
     7501 acgcattgtc gggcgtgtga attttagttt atgggaaact gatacgcgat tttcatcaag
     7561 tgaagataat tcgaattcaa cacatacctt tccttttttg tttgtgctgt ttacgccaaa
     7621 ggcaacttca cttaataccg caccaagttt gttaacaaat accccgccgt cgaggtcgtt
     7681 aatgaaaaca ttaacatcag tttgatgctg gttcatatta atatctccgt gtagaggtgt
     7741 gttaaggctt cagttgttca ctgcatttaa agcctggttt tgtttgtgaa ttaaaaacta
     7801 tcgctgccgt actgccagta acctgggtcg tccgtgtgat tcgggtttac gttttttctg
     7861 cgattgtctt tgtctttgag agtggaaacc atatttgcta ccgtttctgg tttctttcct
     7921 tcagcttttt ctttcagtgt ctgccctgtt tctgcaatga atggaatgcg gatttccatc
     7981 tggtaaccgt ctgcgccagt ctttctgttt gtggttaata ctttctggag gacgagtccg
     8041 attttctttc cgtgaaactc aggtgcaaca tatttactga cggaaaccat atgttgcgtt
     8101 aactgcccaa ttcctgtgca tcccatcatg gcgtgaataa tgctcgcccc aaatttattt
     8161 tcagtgccgt cattcttctg aacacaaaca ctgagatact ggattttccg tccgtcgtcg
     8221 gattctccag aaaactcaat gaatcgggca cctttttctg attgcttgag ttctgcttcg
     8281 gtgatgttaa tgatatgcgc acccgtttcg gtaataaaac caccttgtcc ggcggtcagt
     8341 gctgattctt cgttataagt aaaaatcact tcatccatta gttattaacc ccccattgtt
     8401 ctccaaatac aaaaccaatt tccgccagtg cttcgtccat tttgtcgata aactccggca
     8461 ccatctcatc aaaactcgcc atgtactttt catcccgttc gaccacgaca taatgcaggc
     8521 cttcacgctt catgcgtggg tcgtagttgg cgaagtacca ggcgtccttg tctgtaaccc
     8581 acatgctgaa ttgcacctgg gccatgtaag caggcttgat agcgtcaaag ccgccaagcc
     8641 ggaatttcat gaagtcgcgg gaagtaaaag ggcatttaag ctcaagaccg ttaccatcgc
     8701 tgcataaacc atcgggagag caggcggtgc gcatgctttc gtcacgaaaa attatgggtg
     8761 tttccgagac ggtaacatcc gcgataaatt caaagagggc acgggcatca tcttcatatt
     8821 gctttcccca tgcgagcgac ttagcgttaa cttccggtgc cacgccagta caaacttcag
     8881 ccagcagggt gtggaagtat gacatttttg tatcaggcca tttgctgccg gaacgtggct
     8941 ttgctatcac attgtgaact tctgaagcag tgataacacc gagtctcagc ttgtgccatc
     9001 catcatcgcc ctggtcgagg ctggtaatat ccacgccagt tcgctggaga ataatttctg
     9061 gtgtcatgac acggcctcgc tgttattttc cgtggtggcg tgaattttcg cttccgccgt
     9121 ggccttgttt ctggcagctt tctttttgac aaaatcaagc gttttgacag cttcttcctg
     9181 actgagatat tcatgtgatg caatcgtgcg acggaatgtt ttggaacata agggaagcag
     9241 gtcatcccat gtcttgttaa tttcggtgat tgcctgagtg atctcattga taatctcatc
     9301 tgatgcggga gtgacgtcac gctcagggat gtgatcagca ttcaggatga taccttcacc
     9361 ggcctgagtg ctcaggtagt cgatagctgt atccaggcga tcgcgacggg gccagtattt
     9421 gctggcgcgc ttaacaatgg cttttctggc catttcgtcg gggaagctat cccacgggct
     9481 acttcctccg ttatttccgg ctttgctgca cgccctgatg acctcgattt ccctgttgct
     9541 catctcttcg gtcagatagt cgccttcgga tgtttttacg acacaataac cgccaatgcg
     9601 tgcgcccctg tctacgaatg ggttgtattt gtgcgtgggg gcgcagtcaa taccgttgga
     9661 ctcgtaaatg tccttctcgt aaacaagttt gcattgcccc cactgaatgg ctcctgtgac
     9721 ctgtgccaga tgcagaagcc ccatataact gatatccagg catacagcct tttttcgtgg
     9781 gaccagatac gccagcttgc tggccgggtt cagggttatg ccgatggcgg caacattgat
     9841 gatagcgttc tgtgcgctgg gcagatttgc ccgtgctgtg tcagccagaa aggcattttt
     9901 ctggaattgc tgaattgcaa actgactttc cttcgcccat gtcagcgtcg gttcagttaa
     9961 tgcctcgcag aaaaagcgct cctgctgctt aacaaattca acgatatcga acattttttt
    10021 ggtcctgaaa atcagaaagg acagggggag aattttctct cccattcttc ttccgcccga
    10081 gcataggcga tcgctgagat ataatcgttg tacgcctctt cagctttttc gccagtgagt
    10141 gccagctggg cttctttggg taaaaaaagg ctgctcataa gcaatggttt atcggggaac
    10201 atgctgataa gctcctgcgc ccgatcatca atccatttat ccttttcatc ctgaatttgc
    10261 tgattaatcc agcgacgctc ctctatgcgg tcgcaggtga ggtatgcgtt catggcggaa
    10321 ctcctgattc cggttaatgc attaaattaa tttgtcggga aagctgacat acagggcagt
    10381 tacattcttc ctcctgctcc ttagcgaaga aatatgcagc ggcctgtaat gcgatgtctt
    10441 ctggatgttc cgcgataaac ataacattgc cttccgtatc aataacagaa atagcctcat
    10501 cagacaggat gacaaaatag gcgatgattt tatcatccat aaaaacttct cccattatcg
    10561 ttcctgctgg agttacgacg ctttttacat tgatatttat tt
//
LOCUS       DAZFLD010000162        10593 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.163, whole genome shotgun sequence.
ACCESSION   DAZFLD010000162 DAZFLD010000000
VERSION     DAZFLD010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10593
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.163"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..2076
                     /locus_tag="ACHKBL_003639"
     CDS             <1..2076
                     /locus_tag="ACHKBL_003639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HFO5820456.1"
                     /translation="IISTFHAEKVINDLLKNDYKISLSPDTTYRELNKAAQRSITAPD
                     RIGEGKTWVYQRDTMVERGDNRGVHQYGPAEHFTHIISDKPSPKDKYVAYAINIPDYE
                     LAADVYNINVTSPSGQQETFKILINPEHLRQTLERKSLTAVQKSQCEIITPKKPGEAI
                     LHAFNATYQQIRENMSEFARSHYGYIQIPPVTTFRADGPETPEEEKGYWFHAYQPEDL
                     CTIHNPMGDLQDFIALVKDAKKFGIDIIPDYTFNFMGIGGSGKNDLDYPSADIRAKIS
                     KDIESGIPGYWQGQVLIPFTIDPVTKERKQIHPEDIHLTAKDFETSKDNISKDEWENL
                     HALKEKRLNGMPKTTPKSDQVIMLQNQYVREMRKYGVRGLRYDAAKHSKHEQIERSIT
                     PPLTIYNERLHNTNLFKPIYHEKAVMNYMEYLVTCQLDEEQMSSLLYERDDLSAIDFS
                     LLMKTIKAFSFGGDLQTLASKPGSTISSIPSKRRILININHDFPNNGNLFNDFLFNHQ
                     QDEQLAMAYMAALPFSRPLVYWDGQVLKSTTEIKNYDGSTRVGGEAWLNKGCSTYQQL
                     YNEFHALYIDKAGIWSAFEGVFATKNVLAFSRGDSVNINHSPHDGLVIINKGNEEVEG
                     TWPNKLQPGIYKNMGSNSVNIIINNTRKIIPPGKAFMLRGGTLNINIPGRSALLLGKT
                     GEPPNYLYL"
     gene            2413..3390
                     /gene="glaH"
                     /locus_tag="ACHKBL_003640"
     CDS             2413..3390
                     /gene="glaH"
                     /locus_tag="ACHKBL_003640"
                     /EC_number="1.14.11.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050498 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, with 2-oxoglutarate as one donor, and
                     the other dehydrogenated [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutarate dioxygenase GlaH"
                     /protein_id="HFO5820457.1"
                     /translation="MNALTAVQNNAVDSGQDYSGFTLIPSAQSPRLLELTFTEQTTKQ
                     FLEQVAEWPVQALEYKSFLRFRVGKILDDLCANQLQPLLLKTLLNRAEGALLINAVGV
                     DDVKQADEMVKLATAVAHLIGRSNFDAMSGQYYARFVVKNVDNSDSYLRQPHRVMELH
                     NDGTYVEEITDYVLMMKIDEQNMQGGNSLLLHLDDWEHLDHYFRHPLARRPMRFAAPP
                     SKNVSKDVFHPVFDVDQQGRPVMRYIDQFVQPKDFEEGVWLSELSDAIETSKGILSVP
                     VPVGKFLLINNLFWLHGRDRFTPHPDLRRELMRQRGYFAYATNHYQTHQ"
     gene            3410..4678
                     /gene="lhgO"
                     /locus_tag="ACHKBL_003641"
     CDS             3410..4678
                     /gene="lhgO"
                     /locus_tag="ACHKBL_003641"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HFO5820458.1"
                     /translation="MYDFVIIGGGIIGMSTAMQLIDVYPDARIALQEKESGPACHQTG
                     HNSGVIHAGVYYTPGSLKAQFCLAGNRATKAFCDQNGIRYDNCGKMLVATSELEMERM
                     RALWERTAANGIEREWLNAEELREREPNITGLGGIFVPSSGIVSYREVTAAMAKIFQA
                     RGGEIIYNAEVSALSEHKNGVVIRTRQGGEYEASTLISCSGLMADRLVKMLGLEPGFI
                     ICPFRGEYFRLAPEHNQIVNHLIYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKR
                     EGYRKRDFSFSDTLEILGSSGIRRVLQNHLRSGLGEMKNSLCKSGYLRLVQKYCPRLS
                     LSDLQPWPAGVRAQAVSPDGKLIDDFLFVTTPRTIHTCNAPSPAATSAIPIGAHIVSK
                     VQTLLASQSNPGRTLRAARSVDALHAAFNQ"
     gene            4701..6149
                     /gene="gabD"
                     /locus_tag="ACHKBL_003642"
     CDS             4701..6149
                     /gene="gabD"
                     /locus_tag="ACHKBL_003642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009013 - succinate-semialdehyde
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0009450 - gamma-aminobutyric acid catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HFO5820459.1"
                     /translation="MKPNDSNLFRQQALINGEWLDANNGEVIDVTNPANGDKLGSVPK
                     MGADETRAAIDAANRALPAWRALTAKERATILRNWFNLMMEHQDDLARLMTLEQGKPL
                     AEAKGEISYAASFIEWFAEEGKRIYGDTIPGHQADKRLIVIKQPIGVTAAITPWNFPA
                     AMITRKAGPALAAGCTMVLKPASQTPFSALALAELAIRAGVPAGVFNVVTGSAGAVGN
                     ELTSNPLVRKLSFTGSTEIGRQLMEQCAKDIKKVSLELGGNAPFIVFDDADLDKAVEG
                     ALASKFRNAGQTCVCANRLYVQDGVYDRFAEKLQQAVSKLHIGDGLENGVTIGPLIDE
                     KAVAKVEEHIADALEKGARVVCGGKADERGGNFFQPTILVDVPANAKVSKEETFGPLA
                     PLFRFKDEADVIAQANDTEFGLAAYFYARDLSRVFRVGEALEYGIVGINTGIISNEVA
                     PFGGIKASGLGREGSKYGIEDYLEIKYMCIGL"
     gene            6163..7443
                     /gene="gabT"
                     /locus_tag="ACHKBL_003643"
     CDS             6163..7443
                     /gene="gabT"
                     /locus_tag="ACHKBL_003643"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009448 - gamma-aminobutyric acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HFO5820460.1"
                     /translation="MSSNKELMQRRSQAIPRGVGQIHPIFADRAENCRVWDVEGREYI
                     DFAGGIAVLNTGHLHPKVVAAVEAQLKKLSHTCFQVLAYEPYLELCEIMNQKVPGDFA
                     KKTLLVTTGSEAVENAVKIARAATKRSGTIAFSGAYHGRTHYTLALTGKVNPYSAGMG
                     LMPGHVYRALYPCPLHGISEDDAIASIHRIFKNDAAPEDIAAIVIEPVQGEGGFYAAT
                     PAFMQRLRALCDEHGIMLIADEVQSGAGRTGTLFAMEQMGVAPDLTTFAKSIAGGFPL
                     AGVTGRAEVMDAVAPGGLGGTYAGNPIACVAALEVLKVFEQENLLQKANDLGHKLKDG
                     LLAIAEKHPEIGDVRGLGAMIAIELFEDGDHSKPDAKLTAEIVARARDKGLILLSCGP
                     YYNVLRILVPLTIEDAQIRQGLEIISQCFDEAKQ"
     gene            7681..9081
                     /gene="gabP"
                     /locus_tag="ACHKBL_003644"
     CDS             7681..9081
                     /gene="gabP"
                     /locus_tag="ACHKBL_003644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015185 - gamma-aminobutyric acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GABA permease"
                     /protein_id="HFO5820461.1"
                     /translation="MGQSSQPHELGGGLKSRHVTMLSIAGVIGASLFVGSSVAIAEAG
                     PAVLLAYLFAGLLVVMIMRMLAEMAVATPDTGSFSTYADKAIGRWAGYTIGWLYWWFW
                     VLVIPLEANIAAMILHSWVPGIPIWLFSLVITLALTGSNLLSVKNYGEFEFWLALCKV
                     IAILAFIFLGAVAISGFYPYAEVSGISRLWDSGGFMPNGFGAVLSAMLITMFSFMGAE
                     IVTIAAAESDTPEKHIVRATNSVIWRISIFYLCSIFVVVALIPWNMPGLKAVGSYRSV
                     LELLNIPHAKLIMDCVILLSVTSCLNSALYTASRMLYSLSRRGDAPAVMGKINRSKTP
                     YVAVLLSTGAAFLTVVVNYYAPAKVFKFLIDSSGAIALLVYLVIAVSQLRMRKILRAE
                     GSEIRLRMWLYPWLTWLVIGFITFVLVVMLFRPAQQLEVISTGLLAIGIICTVPIMAR
                     WKKLVLWQKTPVHNTR"
     gene            9102..9764
                     /gene="csiR"
                     /locus_tag="ACHKBL_003645"
     CDS             9102..9764
                     /gene="csiR"
                     /locus_tag="ACHKBL_003645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CsiR"
                     /protein_id="HFO5820462.1"
                     /translation="MTITSLDGYRWLKNDIIRGNFQPDEKLRMSLLTSRYALGVGPLR
                     EALSQLVAERLVTVVNQKGYRVASMSEQELLDIFDARANMEAMLVSLAIARGGDEWEA
                     DVLAKAHLLSKLEACDASEKMLDEWDLRHQAFHTAIVAGCGSHYLLQMRERLFDLAAR
                     YRFIWLRRTVLSVEMLEDKRDQHQTLTAAVLARDTARASELMRQHLLTPIPIIQQAMA
                     GN"
     gene            complement(9765..10214)
                     /gene="kbp"
                     /locus_tag="ACHKBL_003646"
     CDS             complement(9765..10214)
                     /gene="kbp"
                     /locus_tag="ACHKBL_003646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium binding protein Kbp"
                     /protein_id="HFO5820463.1"
                     /translation="MGLFNFVKDAGEKLWDAVTGQHDKDDQAKKVQEHLNKTGIPDAD
                     KVNIQIADGKATVTGDGLSQEAKEKILVAVGNISGIASVDDQVKTATPAAASQFYTVK
                     SGDTLSAISKQVYGNANLYNKIFEANKPMLKSPDKIYPGQVLRIPEE"
     gene            complement(10298..10456)
                     /gene="yqaE"
                     /locus_tag="ACHKBL_003647"
     CDS             complement(10298..10456)
                     /gene="yqaE"
                     /locus_tag="ACHKBL_003647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HFO5820464.1"
                     /translation="MGFWRIVITIILPPLGVLLGKGFGWAFIINILLTLLGYIPGLIH
                     AFWVQTRD"
BASE COUNT         2641 a         2629 c         2763 g         2560 t
ORIGIN      
        1 attatatcaa cttttcacgc agaaaaagtc attaatgatc tgttaaaaaa cgattataaa
       61 atttcccttt ctccagatac aacttaccga gagctgaata aagctgcaca gcgtagcatt
      121 acagcgccag acaggatagg agaagggaaa acatgggttt atcaacgaga tacaatggtt
      181 gaaagaggtg ataacagagg cgttcatcag tatggtccag ctgaacattt cactcacatt
      241 atatctgaca aaccatcccc aaaagataag tatgttgcat atgctattaa cattcctgac
      301 tatgagctag cagccgatgt atataatatt aacgtgacgt caccttccgg acagcaagaa
      361 acatttaaaa tactaatcaa tccagaacat ctacggcaaa cacttgagcg taaatctctt
      421 actgctgttc agaaatcaca atgtgaaatc atcaccccca aaaaacctgg cgaagcgatt
      481 cttcatgctt ttaatgccac ctaccagcaa atcagggaaa atatgtctga gtttgcacgt
      541 tcccattatg ggtatataca aattcctcca gtgacaactt tccgcgccga cggaccagaa
      601 actcccgaag aagaaaaagg ttactggttt cacgcttatc aacccgaaga tctttgtacc
      661 atccataatc caatgggaga tttgcaggat tttatcgcat tggttaaaga tgctaaaaaa
      721 tttggtatcg atatcattcc tgattatacc tttaacttta tgggaattgg gggtagtggt
      781 aaaaatgacc tagattatcc ctctgctgat atacgagcga agatcagtaa agatatagaa
      841 agtggtatcc ctggctattg gcaaggtcag gttttgattc catttactat agatccagta
      901 acaaaagaac gtaaacaaat ccatccagaa gatatacatc tcactgcaaa agactttgaa
      961 acaagtaaag ataacatctc taaggatgaa tgggaaaacc tccatgcatt aaaagaaaag
     1021 cgtttaaatg gaatgcctaa aacaaccccc aaaagtgacc aggttattat gttgcaaaat
     1081 caatacgttc gtgaaatgcg aaaatatggc gtacgaggtt tacgttacga tgcagcaaaa
     1141 cactcaaaac atgaacaaat agaaagatca ataactccac cgcttacaat ttataatgag
     1201 cgattacaca atactaactt atttaagcca atatatcatg aaaaagccgt tatgaattac
     1261 atggaatacc tggtaacttg tcagttggat gaagaacaaa tgtcatcgct gctttatgaa
     1321 agagatgatt taagcgccat tgatttttca ttgctcatga agacgataaa agccttttca
     1381 tttggtggcg atctccaaac ccttgcatca aaaccgggtt caacaatctc aagcatcccg
     1441 tcaaaaagac ggatattgat taacattaac cacgattttc ctaacaatgg caatcttttc
     1501 aatgactttc tatttaacca tcaacaagat gaacaattag caatggcata tatggccgct
     1561 ctcccgttca gcaggccttt agtttactgg gatggccaag tattaaaatc aacgactgaa
     1621 attaaaaatt atgatgggtc gacgcgtgtc ggcggtgagg cgtggcttaa taaaggttgc
     1681 tctacctatc agcagctcta caatgaattc cacgcattat atatagataa agcaggaata
     1741 tggagcgcat ttgagggtgt atttgcaact aaaaacgttc tggcctttag tcgtggggat
     1801 tctgtgaaca ttaatcactc tcctcatgat ggactagtta taataaataa aggaaacgaa
     1861 gaagttgaag gtacctggcc taacaaattg caacctggaa tatataaaaa catggggagt
     1921 aatagcgtta acattattat taataatacc cgaaaaatta tcccccctgg taaagcattt
     1981 atgcttagag gcggaactct aaatatcaat attcctgggc gtagcgctct tcttttaggg
     2041 aaaactggag aaccgccgaa ctatctctat ttgtaattta atgttatatc tgccccgcta
     2101 aaacggggca gataatatgt ttagtttact aacggtcatt ttgcagtgaa gccatttacc
     2161 gttttttatc gaccagataa tctgttctct gatggtaatc cacccctaac ctgttgcttt
     2221 agttattcat ttcctgtctc actttgcctt aataacctac gtcaaatgtt accaatttgt
     2281 tgtttttgat cgcaataaga aaacaatatg taacttttat gcgcattttt cagaaatgta
     2341 gatattttta gattatggct acgaaatgag catcgccatg tcaccctaca tctcataaga
     2401 ggatcgcttc tgatgaatgc actgaccgcc gtacaaaata acgctgtcga ttcaggccag
     2461 gactatagcg gattcactct catcccgtcg gcgcaatctc cgcgtctgct ggaactcacc
     2521 ttcaccgaac agacgaccaa acagtttctc gaacaggttg ccgaatggcc cgtgcaggcg
     2581 ctggagtaca aatcttttct gcgttttcgg gtaggcaaaa ttcttgacga tctgtgtgcg
     2641 aaccagctgc aaccattgct gttgaagacc ctgctaaacc gtgctgaagg tgcgctgttg
     2701 atcaatgcgg tgggtgtcga tgatgtgaag caagcggatg agatggtgaa gctggcaacg
     2761 gcggtagcgc atctgattgg tcgctcaaat ttcgatgcga tgagcggtca gtattacgcg
     2821 cgattcgtgg tgaaaaatgt tgataactca gacagctatc tgcgtcagcc gcaccgcgta
     2881 atggagctgc acaacgacgg cacctacgtc gaagagatca ccgactatgt gctgatgatg
     2941 aaaatcgacg agcagaacat gcagggtgga aattcgttgc tgctgcatct cgatgactgg
     3001 gaacatctgg accactattt ccgccacccg ctggcgcgtc gcccgatgcg ctttgccgcg
     3061 cctccgagca aaaacgtcag caaagatgtt ttccatccag tgttcgacgt tgatcaacag
     3121 ggtcgcccgg tgatgcgtta tatcgaccag ttcgtccagc caaaagactt cgaagaaggc
     3181 gtgtggctga gtgagctttc cgatgccatt gaaaccagca aaggcattct ttctgtgccc
     3241 gttcccgttg gcaaattcct gttgattaac aatctattct ggctgcacgg tcgcgaccgc
     3301 tttactccgc acccggatct gcgccgtgaa ctgatgcgtc agcgtggcta tttcgcttac
     3361 gccactaacc actaccagac gcatcagtaa gcgcaaagga attgagcgga tgtatgattt
     3421 tgtgattatt ggcggcggca tcatcggcat gtcgaccgcc atgcaactga ttgatgttta
     3481 tccggatgcc cgcattgcgt tgcaggaaaa agagtccggc ccggcctgtc accagacggg
     3541 ccacaacagt ggcgtgatcc atgccggggt ctattacacg cccggtagcc tgaaggcaca
     3601 gttttgcctg gcgggaaacc gcgccaccaa agccttttgc gatcaaaacg gcattcgcta
     3661 tgacaactgc ggcaagatgc tggtcgccac ctctgaactc gaaatggaac ggatgcgcgc
     3721 gttgtgggaa cgcacggcag cgaacggtat cgagcgcgag tggttaaacg ccgaggaact
     3781 gcgcgagcgc gaaccgaata tcaccgggct cggcggcatt tttgtgccgt ccagcggcat
     3841 tgtcagctac cgcgaagtga cggcggcgat ggcaaaaatc ttccaggcca gaggcggcga
     3901 gattatttat aacgccgaag tcagcgccct cagtgagcat aaaaacggcg tggtgatacg
     3961 tacccgtcag ggcggtgaat atgaagcatc gacgctgatt agctgttccg ggctgatggc
     4021 tgaccggctg gtgaaaatgc tcggcctcga accgggcttt attatctgcc cgttccgtgg
     4081 cgagtatttc cgccttgcgc cggagcataa ccagattgtt aaccacctga tttaccccat
     4141 tcccgacccc gcgatgccat ttttgggcgt gcatctcacc cgcatgatcg atggcagcgt
     4201 gaccgtcggg ccaaacgcgg tgctggcttt caaacgcgaa ggctatcgta agcgcgactt
     4261 ctcatttagc gacacgctgg agattttggg ctcgtcgggg attcgccggg tgctgcaaaa
     4321 ccatctacgc tcaggactgg gcgagatgaa aaactcgctg tgcaaaagcg gctatctgcg
     4381 gctggtgcaa aagtattgtc cccggctttc gttaagcgat ctccagccct ggcccgctgg
     4441 tgtgcgggcg caggcggtat cgccggacgg caagctgatt gacgattttc tgtttgtcac
     4501 caccccgcgc acgatccaca cctgcaatgc gccctccccg gcagcgacat cagcaattcc
     4561 tattggtgcg catattgtca gcaaggtaca aacgctgttg gcaagccaga gtaaccccgg
     4621 acgcacgctg cgagcggcac gtagtgtgga tgccttacac gccgcattta atcaataacc
     4681 tttgaaaaca ggacgtaaca atgaaaccta acgacagtaa cttattccgc cagcaggcgt
     4741 tgattaacgg ggaatggctg gacgccaaca atggcgaggt catcgacgtc accaatccgg
     4801 cgaacggcga caagctgggt agcgtaccca aaatgggcgc tgatgaaacc cgcgccgcta
     4861 tcgacgccgc caaccgcgct ctgcccgcct ggcgtgcgct caccgccaaa gaacgcgcca
     4921 ccattctgcg caactggttc aatttgatga tggagcatca ggacgattta gcgcgcctga
     4981 tgaccctaga acagggcaaa ccgctggccg aagcgaaagg cgaaattagc tacgccgcct
     5041 cctttattga gtggtttgcc gaagaaggca aacgcattta tggcgacacc attcctggtc
     5101 atcaggccga taaacgcctg attgttatca agcagccgat tggcgtcacc gcggctatca
     5161 cgccgtggaa cttcccggcg gcgatgatta cccgcaaagc cggtccggcg ctggcggcag
     5221 gctgcaccat ggtgctgaag cccgccagtc agacgccgtt ctctgcgctg gcgctggcgg
     5281 agctggcgat tcgcgcaggc gttccggctg gcgtatttaa cgtggtcacc ggttcggcgg
     5341 gcgcggtggg taacgaactg accagcaatc cgctggtgcg caagctgtcg tttaccggct
     5401 cgaccgaaat tggccgccaa ttaatggaac agtgcgcgaa agacatcaag aaagtgtcgc
     5461 tggaactggg cggcaacgcg ccgtttatcg tctttgacga tgccgacctc gacaaagccg
     5521 tggaaggcgc actggcctcg aaattccgca acgccgggca aacctgcgtc tgcgccaacc
     5581 gtttatacgt gcaggacggc gtgtatgacc gctttgccga aaaattacag caggcggtga
     5641 gcaaactgca catcggcgac gggctggaga atggcgtcac catcgggccg ctgattgatg
     5701 aaaaagcggt agcaaaagtg gaagagcata ttgccgatgc gctggagaaa ggtgcgcgtg
     5761 tggtttgcgg cggtaaagcg gatgaacgcg gcggcaactt cttccagccg accattctgg
     5821 tggacgttcc ggccaacgcc aaagtgtcga aagaagagac gttcggcccc cttgccccgc
     5881 tattccgctt taaagatgaa gccgatgtga ttgcgcaggc caatgacacc gagtttggcc
     5941 tggccgccta tttctacgcc cgtgatttaa gccgcgtctt ccgcgtgggc gaggcgctgg
     6001 agtacggcat cgtcggcatt aatactggca ttatttccaa tgaagtggcc ccgttcggtg
     6061 gcattaaagc ctcgggtctg ggtcgtgaag gttcgaagta tggcatcgaa gattacttag
     6121 aaatcaaata tatgtgcatc ggtctttaac tggagaacgc gaatgagcag caataaagag
     6181 ttaatgcagc gccgcagtca ggcgattccc cgtggcgttg ggcaaattca ccccattttc
     6241 gctgaccgcg cggaaaactg ccgggtgtgg gacgttgaag gccgtgagta tattgatttc
     6301 gcgggcggca ttgcggtgct caataccggg cacctgcatc cgaaagtggt tgcggcggtg
     6361 gaagcgcagt tgaaaaagct gtcgcacacc tgcttccagg tgctggctta cgaaccgtat
     6421 ctggagctgt gcgagattat gaatcagaag gtgccgggag atttcgccaa aaaaacgctg
     6481 ctggtaacga ccggttctga agcggtggag aacgcggtga aaatcgcccg cgccgccacc
     6541 aaacgcagcg ggaccatcgc ctttagcggc gcgtatcacg ggcgcacgca ttacacgctg
     6601 gcgctgaccg gcaaggtgaa tccgtactct gcgggcatgg gactgatgcc tgggcacgtt
     6661 tatcgcgcgc tttatccttg cccgctgcac ggcatcagtg aagatgacgc tatcgccagc
     6721 attcaccgaa tttttaaaaa tgacgctgcg ccggaagata tcgccgccat cgtgattgag
     6781 ccggttcagg gcgaaggcgg tttctacgcc gcgacgccag cctttatgca gcgtttacgc
     6841 gcgctgtgtg acgagcacgg gatcatgctg attgccgatg aagtgcagag cggcgcgggg
     6901 cgtaccggca cgctgtttgc gatggagcag atgggcgttg cgccggatct taccaccttt
     6961 gcgaaatcga tcgcgggcgg cttcccgctg gcgggcgtca ccgggcgcgc ggaagtgatg
     7021 gatgccgtcg ctccaggcgg tctgggcggc acctatgccg gtaacccgat tgcctgcgtg
     7081 gcagcgctgg aagtgttaaa ggtattcgag caggaaaatc tgctgcaaaa agccaacgac
     7141 ctggggcata agctgaaaga cggattgctg gcgatagccg aaaaacaccc ggagatcggc
     7201 gatgtacgcg ggctgggagc gatgattgcc atcgagctgt ttgaagatgg cgatcacagc
     7261 aaaccggacg ccaaactcac cgccgagatc gtggcgcgtg cccgcgataa aggcctgatt
     7321 cttctctcct gcggcccgta ttacaacgtg ctgcgcatcc ttgtaccgct caccattgaa
     7381 gacgctcaga tccgtcaggg tctggaaatc atcagccagt gttttgatga ggcgaagcag
     7441 tagatccgta cccatgccgg aggcgacgct gcgcgtcttg tccggcctac gcgaatccag
     7501 gtcggataaa gcgtttacgc tgcatccgac aatctgtacg tgaacaggaa taaatctatg
     7561 tcggccggat aaggcggagc cgctctccgg taaaaagaac caataacaat tacacgcgtg
     7621 acccgacgcg ggaaatgtcg gggcgctctc ccaagtgaca cactttcgag aggattcagg
     7681 atggggcaat catcgcaacc acatgagtta ggcggcgggc tgaagtcacg ccacgtcacc
     7741 atgttgtcta ttgccggtgt tatcggcgca agtctgtttg tcggttccag cgtcgccatc
     7801 gccgaagcgg gcccggcggt attactggcc tatctgttcg ccggattact ggtggttatg
     7861 attatgcgga tgttggcgga aatggcggtt gccacgcccg ataccggttc gttttccacc
     7921 tatgccgaca aggcaattgg ccgctgggcg ggttatacca tcggctggct gtactggtgg
     7981 ttttgggtac tggttatccc gctggaagcc aacatcgccg ccatgatcct gcactcgtgg
     8041 gttccaggca ttcccatctg gttattttcc ctggtcatta ccctcgcctt gactggcagt
     8101 aatttattaa gcgttaaaaa ctacggcgaa tttgagttct ggctggcgct gtgcaaagtc
     8161 atcgctatcc tggcctttat tttccttggt gcagtcgcaa ttagcggttt ttacccttat
     8221 gccgaagtga gcgggatctc aagattgtgg gatagcggcg gctttatgcc caacggcttc
     8281 ggcgcagtat taagcgcgat gttgatcacc atgttctcgt ttatgggcgc agaaattgtc
     8341 accattgccg ccgcggaatc tgacacgccg gaaaaacata ttgtccgcgc caccaactcg
     8401 gttatctggc gtatttctat cttctattta tgctctattt ttgtcgtggt ggcattaata
     8461 ccgtggaata tgccggggct gaaagccgtt ggatcttatc gctcggttct ggagttgctc
     8521 aatattcccc atgcgaaatt aatcatggac tgcgtgatat tactttccgt aaccagttgc
     8581 ctgaactcgg cgctgtatac cgcttccaga atgctctact ccttaagccg tcgcggtgat
     8641 gctcccgcgg taatgggtaa aatcaaccgc agtaaaaccc cgtatgtggc ggtattactc
     8701 tcgaccggtg cggcattttt aacggtggtg gtgaattatt acgcgcctgc gaaagtgttt
     8761 aaatttctga tcgatagctc cggcgctatc gccctgctgg tttatttagt cattgccgtt
     8821 tcacaattgc ggatgcgcaa aattctgcga gcagaaggaa gcgaaattcg cctgcggatg
     8881 tggctttatc cgtggctcac ctggctggtc atcggtttta taacctttgt gttggtagtg
     8941 atgctattcc gcccggcgca acagttagaa gtgatttcca ccggtttatt agcgataggg
     9001 ataatctgta ccgtgccaat tatggctcgc tggaaaaagc tggtattgtg gcaaaaaaca
     9061 cccgttcata atacgcgcta ataatgatca ggagtcacac catgaccatt acgtctctgg
     9121 atggctatcg ctggctgaag aacgatatta ttcgcggaaa ctttcaaccg gatgaaaaat
     9181 tacggatgag tttgctgaca tcgcgttatg cacttggcgt tgggccgttg cgggaagcgc
     9241 tttcgcaact ggtagcagaa cgactggtca cggtggtgaa tcaaaaaggg tatcgggtgg
     9301 cgtctatgtc agagcaagag ctgctcgata ttttcgacgc ccgcgccaat atggaagcga
     9361 tgctggtcag tctggcgatt gcccgcggtg gcgatgaatg ggaggcagac gttctcgcaa
     9421 aagcgcatct gctcagtaag ctggaggcct gtgacgccag tgagaaaatg cttgatgagt
     9481 gggatctgcg tcatcaggcg tttcatactg caattgtggc gggctgtggt tctcactatt
     9541 tgctgcaaat gcgtgaacgg ttgtttgatc tggcggcgcg ttatcgattt atctggctgc
     9601 ggcgaacagt gctttcggtg gaaatgctgg aagataaacg cgatcagcac cagaccctca
     9661 ctgcggcggt actggcgcga gataccgcgc gcgccagtga gttaatgcgc cagcatttac
     9721 tgacgccaat tcccattatc cagcaggcga tggctggcaa ttaactactc ttcaggaata
     9781 cgcaacactt gccccgggta aattttatcc gggcttttga gcatcggttt attcgcttcg
     9841 aagattttat tgtacagatt agcgttaccg tagacctgtt tggaaatggc actcagagtg
     9901 tcgccagact taacggtata aaactggctg gcagcggctg gtgtcgccgt tttcacctga
     9961 tcatcgacac tggcaatacc ggaaatattc cccaccgcaa caaggatttt ctccttcgcc
    10021 tcctgactta agccgtcacc cgtgaccgtc gctttgccgt cggcgatttg aatgttcact
    10081 ttatcggcat caggtattcc ggttttgttc agatgctcct gcaccttctt cgcctgatcg
    10141 tctttatcgt gctgacctgt aaccgcgtcc cagagttttt ctccggcatc tttcacaaaa
    10201 ttgaacagac ccatagctac ctcattcgtt aacggaaaca ctcagaaagt gtagcagagg
    10261 aacgcggcgc gttaacgccg cgggggtgaa cacaggttta atcgcgggtt tgcacccaga
    10321 acgcgtgaat caaaccgggg atatacccaa gcagtgtcag cagaatatta ataatgaacg
    10381 cccaaccgaa ccctttaccg agcagcacgc cgagcggcgg cagaatgatg gtgatgacga
    10441 ttctccagaa acccatatgt actccctata agaaaattac tcattgttta aaaagagatt
    10501 ttatctctta agcgtagtaa ttttagcgga gcctgccagt ttcagcttac gcttttactc
    10561 tcatttacgc tgctgaggct ggtatatgtt gca
//
LOCUS       DAZFLD010000163        10583 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.164, whole genome shotgun sequence.
ACCESSION   DAZFLD010000163 DAZFLD010000000
VERSION     DAZFLD010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10583
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.164"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            91..1458
                     /gene="dcuC"
                     /locus_tag="ACHKBL_003648"
     CDS             91..1458
                     /gene="dcuC"
                     /locus_tag="ACHKBL_003648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HFO5820465.1"
                     /translation="MFGIIISVIVLITMGYLILKNYKPQVVLAAAGIFLMMCGVWLGF
                     GGVLDPAKSSGYLIVDIYNEILRMLSNRIAGLGLSIMAVGGYARYMERIGASRAMVSL
                     LSRPLKLIRSPYIILSATYVIGQIMAQFITSASGLGMLLMVTLFPTLVSLGVSRLSAV
                     AVIATTMSIEWGILETNSIFAAQVAGMKIATYFFHYQLPVASCIIISVAISHFFVQRA
                     FDKKDKNINHEQAEQKALDNVPPLYYAILPVMPLILMLGSLFLAHVGLMQSELHLVVV
                     MLLSLTVTLFVEFFRKHNLRETMDDVQAFFDGMGTQFANVVTLVVAGEIFAKGLTTIG
                     TVDAVIRGAEHSGLGGIGVMIIMALVIAIGAIVMGSGNAPFMSFASLIPNIAAGLHVP
                     AVVMIMPMHFATTLARAVSPITAVVVVTSGIAGVSPFAVVKRTAIPMAVGFVVNMIAT
                     ITLFY"
     gene            complement(1502..1999)
                     /gene="sspB"
                     /locus_tag="ACHKBL_003649"
     CDS             complement(1502..1999)
                     /gene="sspB"
                     /locus_tag="ACHKBL_003649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HFO5820466.1"
                     /translation="MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPME
                     YARDGQIVLNIAPRAVGNLELANDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGT
                     MFEPEAAYDEDASIMNDEEASADNETVMSVIDGDKPDHDDDTHPDDEPPQPPRGGRPA
                     LRVVK"
     gene            complement(2005..2643)
                     /gene="sspA"
                     /locus_tag="ACHKBL_003650"
     CDS             complement(2005..2643)
                     /gene="sspA"
                     /locus_tag="ACHKBL_003650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein SspA"
                     /protein_id="HFO5820467.1"
                     /translation="MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKD
                     NPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLY
                     MHRIEKDWYTLMNTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCY
                     LAPLLWRLPQLGIEFSGPGAKELKGYMTRVFERDSFLASLTEAEREMRLGRS"
     gene            complement(3038..3430)
                     /gene="rpsI"
                     /locus_tag="ACHKBL_003651"
     CDS             complement(3038..3430)
                     /gene="rpsI"
                     /locus_tag="ACHKBL_003651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFO5820468.1"
                     /translation="MAENQYYGTGRRKSSAARVFIKPGNGKIVINQRSLEQYFGRETA
                     RMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAG
                     FVTRDARQVERKKVGLRKARRRPQFSKR"
     gene            complement(3454..3882)
                     /gene="rplM"
                     /locus_tag="ACHKBL_003652"
     CDS             complement(3454..3882)
                     /gene="rplM"
                     /locus_tag="ACHKBL_003652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HFO5820469.1"
                     /translation="MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEY
                     TPHVDTGDYIIVLNADKVAVTGNKRTDKVYYHHTGHIGGIKQATFEEMIARRPERVIE
                     IAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI"
     gene            complement(4101..5228)
                     /gene="zapE"
                     /locus_tag="ACHKBL_003653"
     CDS             complement(4101..5228)
                     /gene="zapE"
                     /locus_tag="ACHKBL_003653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HFO5820470.1"
                     /translation="MQSVTPTSQYLKALNEGSHQPDDVQKEAVSRLEIIYQELINSTP
                     PAPRTSGLMARVGKLWGKREDTKHTPVRGLYMWGGVGRGKTWLMDLFYQSLPGERKQR
                     LHFHRFMLRVHEELTALQGQTDPLEIIADRFKAETDVLCFDEFFVSDITDAMLLGGLM
                     KALFARGITLVATSNIPPDELYRNGLQRARFLPAIDAIKQHCDVMNVDAGVDYRLRTL
                     TQAHLWLSPLNDETRAQMDKLWLALAGAKRENSPTLEINHRPLATMGVENQTLAVSFT
                     TLCVDARSQHDYIALSRLFHTVMLFDVPVMTRLMESEARRFIALVDEFYERHVKLVVS
                     AEVPLYDIYQGERLKFEFQRCLSRLQEMQSEEYLKREHLAG"
     gene            5422..5820
                     /gene="zapG"
                     /locus_tag="ACHKBL_003654"
     CDS             5422..5820
                     /gene="zapG"
                     /locus_tag="ACHKBL_003654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Z-ring associated protein ZapG"
                     /protein_id="HFO5820471.1"
                     /translation="MTWEYALIGLVVGIIIGAVAMRFGNRKLRQQQALQYELEKNKAE
                     LDEYREELVSHFARSAELLDTMAHDYRQLYQHMAKSSSSLLPELSAEANPFRNRLAES
                     EASNDQAPVQMPRDYSEGASGLLRTGAKRD"
     gene            5974..7341
                     /gene="degQ"
                     /locus_tag="ACHKBL_003655"
     CDS             5974..7341
                     /gene="degQ"
                     /locus_tag="ACHKBL_003655"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegQ"
                     /protein_id="HFO5820472.1"
                     /translation="MKKQTQLLSALALSVGLTLSASFQAVASIPGQVADQAPLPSLAP
                     MLEKVLPAVVSVRVEGTASQGQKIPEEFKKFFGDDLPDQPAQPFEGLGSGVIINASKG
                     YVLTNNHVINQAQKISIQLNDGREFDAKLIGSDDQSDIALLQIQNPSKLTQIAIADSD
                     KLRVGDFAVAVGNPFGLGQTATSGIVSALGRSGLNLEGLENFIQTDASINRGNSGGAL
                     LNLNGELIGINTAILAPGGGSVGIGFAIPSNMARTLAQQLIDFGEIKRGLLGIKGTEM
                     SADIAKAFNLDVQRGAFVSEVLPGSGSAKAGVKAGDIITSLNGKPLNSFAELRSRIAT
                     TEPGTKVKLGLLRNGKPLEVEVTLDTSTSSSASAEMITPALEGATLSDGQLKDGGKGI
                     KIDEVVKGSPAAQAGLQKDDVIIGVNRDRVNSIAEMRKVLAAKPAIIALQIVRGNESI
                     YLLMR"
     gene            7431..8498
                     /gene="degS"
                     /locus_tag="ACHKBL_003656"
     CDS             7431..8498
                     /gene="degS"
                     /locus_tag="ACHKBL_003656"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HFO5820473.1"
                     /translation="MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETP
                     ASYNLAVRRAAPAVVNVYNRGLNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDAD
                     QIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINPRRVPHIGDVVLAIGN
                     PYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTL
                     SFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQSGGM
                     DQLQGIVVNEVSPDGPAANAGIQVNDLIISVNNKPAISALETMDQVAEIRPGSVIPVV
                     VMRDDKQLTLQVTIQEYPATN"
     gene            complement(8561..9499)
                     /gene="mdh"
                     /locus_tag="ACHKBL_003657"
     CDS             complement(8561..9499)
                     /gene="mdh"
                     /locus_tag="ACHKBL_003657"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030060 - L-malate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HFO5820474.1"
                     /translation="MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAV
                     DLSHIPTAVKIKGFSGEDATPALEGADVVLISAGVARKPGMDRSDLFNVNAGIVKNLV
                     QQVAKTCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAE
                     LKGKQPGEVEVPVIGGHSGVTILPLLSQVPGVSFTEQEVADLTKRIQNAGTEVVEAKA
                     GGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFFSQPLLLGKNGVE
                     ERKSIGTLSAFEQNALEGMLDTLKKDIALGEEFVNK"
     gene            9934..10404
                     /gene="argR"
                     /locus_tag="ACHKBL_003658"
     CDS             9934..10404
                     /gene="argR"
                     /locus_tag="ACHKBL_003658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HFO5820475.1"
                     /translation="MRSSAKQEELVKAFKALLKEEKFSSQGEIVAALQEQGFDNINQS
                     KVSRMLTKFGAVRTRNAKMEMVYCLPAELGVPTTSSPLKNLVLDIDYNDAVVVIHTSP
                     GAAQLIARLLDSLGKAEGILGTIAGDDTIFTTPANGFTVKDLYEAILELFDQEL"
BASE COUNT         2601 a         2684 c         2642 g         2656 t
ORIGIN      
        1 ccataaatag ccttgcaaaa aatataacat cattgttttc aatctgccgt ttatgggatt
       61 gaccattttc ttttgacacg gagttcaaca atgttcggca taattatatc tgtcatcgta
      121 ttaattacga tgggctattt gatcctgaaa aactacaaac ctcaggtggt gctggctgcc
      181 gcaggtatct tcctgatgat gtgcggtgtc tggttagggt tcggtggtgt gctcgatccc
      241 gccaaaagca gcggctactt gatcgtcgat atttataatg aaatcctgcg catgctgtcc
      301 aaccgcattg ctggattggg gctgtcgatt atggcagtgg gcggttatgc ccgctatatg
      361 gagcgcatag gggcaagtcg cgcgatggtg agcttgttaa gccgcccgtt aaaactcatt
      421 cgctcgccgt atattattct gtcggcaact tacgtcatcg gccaaatcat ggcgcagttt
      481 attaccagcg cctccggtct gggtatgttg ctgatggtca ccttatttcc gacgctggtg
      541 agtctgggag taagtcgtct ctctgcggtg gcggttatcg caaccaccat gtccattgag
      601 tgggggattc tggaaacgaa ctccattttt gctgcccagg tcgcaggaat gaaaattgcc
      661 acttacttct tccactacca gcttccggtc gcctcttgca tcattatctc ggtggcgatc
      721 tcccactttt tcgtgcaacg cgctttcgac aaaaaagata aaaatatcaa tcacgaacag
      781 gcagagcaaa aagctctcga taatgtcccg ccgctctatt acgccatttt accggtgatg
      841 ccgttaatcc tgatgctcgg ctcgctgttc cttgcccacg tcgggctgat gcagtcagaa
      901 ctgcatctgg tggtggtgat gttactgagt ttgacggtga cgctgtttgt tgagttcttc
      961 cgcaagcata acttgcgcga aacaatggac gatgtgcagg cgttttttga cggcatgggt
     1021 acgcagtttg ccaacgtggt aacgctggtg gtcgcgggtg aaatatttgc gaaaggctta
     1081 acgacgattg gcactgtcga tgcggttatc aggggggcgg agcattctgg tctgggcggt
     1141 attggcgtga tgattattat ggcgctggtc attgccattg gtgccattgt gatgggctca
     1201 ggcaatgcgc cgtttatgtc atttgccagt cttattccga atatcgcagc cggactacat
     1261 gtaccagcgg ttgtaatgat tatgccgatg cattttgcca cgacgctagc gcgcgctgtt
     1321 tcgccgatta ctgcggtggt ggtcgttacg tcaggaattg caggcgtttc gccttttgcg
     1381 gtagtgaagc ggacagcgat ccccatggca gtcggtttcg tggtgaatat gattgccact
     1441 atcacgctat tttattaagc gattaaaaag acaaaacagg ccgccagggc ctgttttcat
     1501 tttacttcac aacgcgtaat gccggtcgac caccgcgtgg tggctgcgga ggttcatcgt
     1561 caggatgagt gtcatcatcg tgatctggct tgtcgccatc aataaccgac ataacggttt
     1621 cgttgtctgc cgatgcctct tcatcattca tgatgctggc atcttcatcg taggcagctt
     1681 caggttcaaa catcgtgcct gcgccatttt cacgggcgta gatagccagc acggcagcca
     1741 gcggcacaga aacctgacgc ggaatgccac caaagcgcgc gttaaagcgc acctcatcat
     1801 tcgccagttc cagattgccg acagctcgcg gcgcaatgtt gagtacgatt tgcccgtcac
     1861 gcgcatattc cataggaacc tgcacgccag ggagcgtcac atccaccacc aggtgcggcg
     1921 tgagctggtt atccagcaac cactcataga atgcacgcag cagataggga cgacgtggtg
     1981 ttagctgtga caaatccata cagattaact ccggcccaga cgcatttcac gttcagcttc
     2041 agttaaagaa gcaaggaaag agtcacgctc aaagacgcgg gtcatatagc ctttcagctc
     2101 tttcgcaccc gggccgctga actcgatgcc cagttgcggc agacgccaca gcagaggagc
     2161 aaggtagcaa tcgaccaggc tgaactcatc gctcaggaag tacggcttct gaccgaagac
     2221 cggcgcaatc gccagcagtt cttcgcgcag ttgcttacgt gcggcatcgg cttcagaagc
     2281 tgaaccgttg atgatggtgt tcatcagcgt gtaccagtct ttttcgatgc gatgcatgta
     2341 cagacggctt tcaccgcgag ctaccgggta aacaggcatc agtggcggat gcgggaaacg
     2401 ctcatccaga tattccataa tgatgcgaga ttcccacagg gtcagctcac gatccaccag
     2461 ggtcggaacg ctctgattcg ggttgaggtc aatcagatcc tgaggcggat tgtccttttc
     2521 cacgtgttcg atctcgaaac ttacaccttt ctcagccagc acaatgcgga cctgatggct
     2581 atagatgtca gtaggaccgg aaaacagcgt cattaccgaa cgtttgttgg cagcgacagc
     2641 catgaaaacc tccaggtata gtcagaattt ttactgctac cagccaccag gtggccagtc
     2701 agaagttgtg ttacccagta aggaacgact ctctttgttc gaaaatcaaa caaaaaatga
     2761 gcaatacccg acatttgggc agaaaattgg atgatagttt accagatttt gcgacctttg
     2821 tggtgagtcg atgccggaaa tggggaaaaa gagatgcgct ttagtctgaa atagttgact
     2881 tagtccctta ttggcgatgt ggtttttgtt ttacctgtct gtcaggtggc agcaaaaagc
     2941 aacttcccat tttttacgct gatgcagatt ttagccataa aaaaacccgc cgaagcgggt
     3001 tttttcgcaa attgttttct gccggagcag aagccaatta acgtttggag aactgcggac
     3061 gacgacgtgc tttacgcaga ccgactttct tacgttcaac ctgacgagcg tcacgagtaa
     3121 cgaagccagc tttacgcagt tcagaacgca gggactcgtc gtattccatc agagcgcggg
     3181 tgataccgtg acggatcgca ccagcctgac cagagatacc accaccttta acggtgatgt
     3241 acaggtccag tttctcaacc atgtcgacca gttccagcgg ctgacgaact accatgcgag
     3301 cagtttcacg accaaagtac tgttccagag aacgttggtt gattacgatt ttaccgttgc
     3361 ccggtttgat gaaaacgcga gctgcggaac ttttgcggcg accagtgccg tagtattgat
     3421 tttcagccat tgcctataat cccgattaga tgattagatg tcaagaactt gcggttgctg
     3481 tgccgcgtgg ttgtgctcgt tacccgcgta aactttcagt ttacggaaca tagcacgacc
     3541 cagcgggcct tttggcaaca tgcctttaac cgcgatttca atcacacgct caggacggcg
     3601 agcaatcatc tcttcaaagg tcgcttgttt gataccaccg atgtggccgg tgtggtgata
     3661 gtacactttg tcagtacgct tgttgccggt tacagcaact ttgtcagcgt tcagaacgat
     3721 gatgtaatca ccggtatcta cgtgcggagt gtattccgct ttgtgcttac cgcgcaggcg
     3781 acgagccagt tcagtagcca gacggcccag agttttaccg gtcgcgtcaa caacatacca
     3841 gtcgcgtttt acggtttctg gtttagctgt aaaagttttc attaaaagct tacccaataa
     3901 aaagttacac gttggtgaac acccaaacgt cttcaattgt tgaggttcac acgacaaagt
     3961 ccggcaaacc taccccttcg aatagcctat gccagcacac aaaaagtttt gggaaaaaaa
     4021 ctttcttgta acgtggggtc gcaggattat agagaagtcg gggtcaaaga tcgacccctt
     4081 tttgtgattt gtggcaggtt ttaacccgcc aaatgctcgc gcttcagata ctcttcgctt
     4141 tgcatctctt gcagacgtga caggcaacgc tggaactcaa atttcagccg ctcgccctga
     4201 taaatgtcat atagcggcac ttctgcactc accactaatt tgacatggcg ctcgtaaaac
     4261 tcatccacca gcgcaataaa acgtcgcgct tcgctctcca tcaaccgcgt cataactggt
     4321 acatcaaaca acatgactgt gtgaaagagg cgtgagagcg caatatagtc atgctgactg
     4381 cgggcgtcga cgcacagcgt agtaaaagag accgccagcg tctggttctc gacgcccatt
     4441 gtcgccagcg gccgatggtt gatttctaat gtcggtgaat tttctcgttt cgcccccgcc
     4501 agcgccaacc atagtttatc catttgcgcc cgggtttcat cgttgagggg cgaaagccac
     4561 agatgcgcct gagtgagtgt acgcagtcga taatcaacac cagcgtccac gttcattaca
     4621 tcacaatgct gtttaatggc atcgattgca ggcaaaaaac gcgcacgttg taggccattt
     4681 cgataaagtt cgtccggcgg aatatttgac gtcgctacca gggtaatacc gcgggcgaac
     4741 agggctttca tcagaccgcc aagtagcatg gcatcggtaa tatcagaaac aaaaaattcg
     4801 tcaaaacaga gcacgtcagt ttctgcttta aagcgatcgg caataatttc cagcggatcg
     4861 gtctgcccct gcaaagcagt tagctcttca tgtacccgca gcataaaacg gtgaaagtgc
     4921 agacgctgtt tccgctctcc cggcaggctt tgatagaaaa ggtccatcag ccaggttttc
     4981 ccgcgtccta caccgcccca catatataag ccacgcactg gcgtatgctt tgtgtcttcg
     5041 cgtttacccc acagcttacc gacccgcgcc attagcccac tcgtcctggg ggctggaggc
     5101 gtgctattga tgagttcctg ataaataatt tccaggcggc tgacggcctc tttttgaacg
     5161 tcgtcgggtt gatggctgcc ttcattgagc gccttcaggt attgcgatgt tggggtaacg
     5221 ctttgcatga tcttattgtt attccttgaa taatcggtgc gccgttgttc acggttgacg
     5281 aaaaaaaggc cgttctacac tacgcgatat gcagtcggga ttccacttct gtggaattaa
     5341 cggttatagt ggcataatca gccgcaggca tggagcctga agccaacacc ctacggaaac
     5401 aaaagacaac gggagatgtt catgacctgg gaatatgcgc taattgggtt agtcgtcggc
     5461 atcattattg gtgctgtggc catgcgtttt ggtaatcgta aactacgcca gcaacaggcg
     5521 ttgcagtacg aactggaaaa gaataaagct gaactggacg agtatcgcga agagctggtt
     5581 agccactttg cccgcagcgc ggaattactg gataccatgg cgcacgacta tcgccagctg
     5641 tatcaacaca tggcgaaaag ctccagcagc ctgctgccgg aactgtctgc tgaagcaaac
     5701 ccgttccgta atcgtctggc agagtctgaa gccagcaacg atcaggcacc ggtgcaaatg
     5761 cctcgcgact attctgaagg cgcatccggc ctactgcgta ctggcgcgaa gcgcgactaa
     5821 tttatttttc gggcgcagcc attgcgccct cctcttctct ccctccccga ctatcattta
     5881 atctggtgtc tcattgttag ccgtctgaaa attcaataac atcaaactgt tttgaatctc
     5941 ttttcttatc attcaggtac gagagtagga ataatgaaaa aacaaaccca gctgttgagt
     6001 gcattagcgt taagtgtcgg gttaactctc tcggcgtcat ttcaggccgt tgcgtcgatt
     6061 ccaggccagg ttgccgatca ggcccctctc cccagtctgg ccccaatgct ggaaaaagtg
     6121 cttccggcag tggtgagcgt acgggtggaa ggaacggcca gtcagggaca gaaaatcccg
     6181 gaagaattca aaaagttttt tggtgatgat ttaccggatc aaccggcaca acccttcgaa
     6241 ggtttaggct ccggtgtcat catcaacgcc agtaaaggct atgtgctgac taacaaccat
     6301 gtgattaatc aggcacagaa aatcagtatt cagctcaatg atgggcgcga gtttgatgca
     6361 aaactgattg gtagcgatga ccagagcgat atcgccctgt tgcaaattca aaacccgagc
     6421 aaattaacgc aaatcgctat tgccgactcc gataaattgc gcgtcggtga ttttgccgtg
     6481 gcggtcggta acccatttgg ccttgggcaa accgccacct ctggcattgt ttccgcatta
     6541 ggccgcagtg ggttgaatct tgaaggtctg gaaaacttta tccagacaga tgcttccatt
     6601 aaccgcggta actccggcgg tgcactgtta aatcttaacg gtgagttaat tggcatcaac
     6661 acggcaatcc ttgcgcctgg tggcgggagc gtcgggattg gatttgccat ccccagtaat
     6721 atggcgcgaa cactggcgca gcagcttatc gactttggtg aaatcaaacg cggtctgtta
     6781 ggcatcaaag gcacagagat gagtgccgat atcgccaaag ccttcaacct tgacgtgcag
     6841 cgtggcgcgt ttgtcagcga agtgttgcca ggttctggct cggcaaaagc gggcgtcaaa
     6901 gcgggcgata ttattaccag cctcaacggc aaaccgctga atagctttgc tgagttgcgc
     6961 tctcgtatcg caaccaccga gccgggcacg aaagtgaagc ttggcctgct gcgtaacggc
     7021 aaaccactgg aagtagaagt gacgctcgat accagcacct cttcgtcggc cagcgctgaa
     7081 atgatcacgc cagcgcttga aggtgcaacg ttgagcgatg gtcagctaaa agatggcggc
     7141 aaaggtatta agatcgatga ggttgtcaaa ggaagcccag ctgctcaggc aggcttgcaa
     7201 aaagacgatg tgatcattgg cgtcaaccgc gatcgggtga actcgattgc tgaaatgcgt
     7261 aaagtgctgg cggcaaaacc ggccatcatc gccctgcaaa ttgtacgcgg caatgaaagc
     7321 atctatctgc tgatgcgtta atgtcgtaaa ccgggcatca ggctgacgtg tgatgtccgg
     7381 ttaactcgtg gtatgctgct gccgttccct tttttaatga cgcctccatc atgtttgtga
     7441 agctcttacg ttccgttgcg attggattaa ttgtcggcgc tattctgctg gttgccatgc
     7501 cttcgctgcg cagccttaac ccgctttcca ctccgcaatt tgacagtacc gatgagacgc
     7561 ctgccagcta taatctggcg gttcgccgcg ccgcgccagc ggtggttaac gtttacaacc
     7621 gtggtttgaa caccaactct cacaaccagc ttgagatccg caccctggga tccggtgtaa
     7681 tcatggatca acgcggttat atcatcacca ataaacacgt catcaacgac gccgatcaga
     7741 tcatcgtcgc cttacaggat ggacgtgttt ttgaagcgtt actggttggc tctgactctc
     7801 taaccgatct ggcggtgctg aaaattaacg ccacaggcgg tttaccaacg attccgatca
     7861 atccacgccg cgtaccgcac attggcgatg tggttctggc gatcggtaac ccgtacaacc
     7921 tcgggcagac cattacccag gggattatca gtgccacggg tcgaatcggt ctgaacccga
     7981 ccgggcggca aaacttcctg caaaccgatg cttccattaa ccacggtaac tctggcggcg
     8041 cgctggtgaa ctcgctgggc gaactgatgg gcattaacac gctgtcgttt gataagagta
     8101 acgatggcga aacgccggaa ggtatcggct ttgcgattcc tttccagtta gcgaccaaaa
     8161 ttatggataa actgatccgt gatggccgtg tgatccgtgg ctacattggc attggtggac
     8221 gtgagatcgc cccattacat gcgcagagtg gcggcatgga tcaactgcaa gggatcgtgg
     8281 ttaacgaagt gtcgcctgat ggcccagcgg caaatgcagg catccaggtc aacgatctga
     8341 ttatctctgt gaataacaaa cccgctattt ccgccctgga gacaatggat caggtcgcgg
     8401 aaattcgccc tggttcggtg atcccggttg tcgtgatgcg tgacgataaa cagttaacat
     8461 tgcaggtcac cattcaggaa tatccggcga ccaactaagg tcagcgtaaa aaaacaaaaa
     8521 ccggagtctg tgctccggtt ttttattatc cgctaatcaa ttacttatta acgaactctt
     8581 cgcccagggc gatatctttc ttcagcgtat ccagcatacc ttccagcgcg ttctgttcaa
     8641 atgcgctcag ggtaccgata gatttacgct cttccacgcc gtttttaccc agcagcagcg
     8701 gttgagagaa gaaacgtgcg tactgaccgt cgccttcaac gtaggcacat tcgacaacgc
     8761 cttgttcgcc ctgcagtgcg cgaaccagag acagaccaaa acgtgcagct gcctggccca
     8821 tagacagggt tgcagacccg ccaccggctt tcgcttcaac cacctcagta cccgcgttct
     8881 ggatacgttt ggtcagatca gccacttcct gctcggtaaa actaacgcca ggaacctgtg
     8941 acagcagcgg cagaatggta acaccagagt gaccgccaat aaccggcact tcaacttcgc
     9001 ctggctgttt gcctttcagt tccgcaacaa aggtgttgga acgaatgata tccagcgtgg
     9061 taacgccgaa cagtttgttt ttgtcataaa caccggcttt tttcagcact tcagcagcaa
     9121 tcgcaactgt ggtgttaacc gggttagtga taataccaat gcacgctttc gggcaggttt
     9181 tcgcaacttg ctgtaccagg tttttcacga tgccggcgtt aacgttaaac aggtcggaac
     9241 gatccatacc cggtttacgc gctacacctg cagagataag aacgacatct gcgccttcca
     9301 gcgccggagt cgcatcttca ccagaaaaac ctttgatttt cacagcagta gggatatggc
     9361 tcagatcgac agccacaccg ggagtcactg gagcgatatc atacagagag agttctgaac
     9421 ctgaaggcag ttgggttttt aacagtagtg caagcgcctg gccaataccg ccagcagcgc
     9481 cgaggactgc gactttcatc ctaaactcct tattatattg ataaactaag atatgttgct
     9541 ccgctgccgc gaccttaatc cacaaaattg ccgtttacaa tgaccacatc tcaagaatgt
     9601 gtagtcacgc aagtttagcg tttatgcatt taattgccgt aatcaggaac ctaacttacg
     9661 taattaacag tcgcttatgc attagcgcaa cattccaaca ggtggtgaca atatacccta
     9721 ccgttcagcc aaaacaacat caatttgata acaattaatt tacttttaag cagaatttgc
     9781 atgccgtgac gcaggcatgt ttctcaataa cgaaatttga taaaatcccg ctctttcata
     9841 acattatttc agccttcttc agggctgact gtttgcataa aaattcatct gtatgcacaa
     9901 taatgttgta tcaaccacca tatcgggtga cttatgcgaa gctcggctaa gcaagaagaa
     9961 ctagttaaag catttaaagc attacttaaa gaagagaaat ttagctccca gggcgaaatc
    10021 gtcgccgcat tgcaggagca aggctttgac aatattaatc agtctaaagt ctcgcggatg
    10081 ttgaccaagt ttggtgctgt acgtacacgc aatgccaaaa tggaaatggt ttactgcctg
    10141 ccagctgaac tgggtgtacc aaccacctcc agtccattga agaatctggt gctggatatc
    10201 gactacaacg atgcagttgt cgtgattcat accagccctg gcgcggcgca gttaattgct
    10261 cgcctgctgg actcactggg caaagcagaa ggtattctgg gcaccatcgc tggcgatgac
    10321 accatcttta ctacccctgc taacggtttc accgtcaaag acctgtacga agcgatttta
    10381 gagctgttcg accaggagct ttaatctctg ccccgtcgct tctggcggcg gggaaaatgt
    10441 tgcttttccc tctcaccccc cctgctttct cctgcgatta atttaacgaa tagtgcgttt
    10501 tatcgcgaca tatcattcac ataatgaata catgggttaa aaaaagcaca ttatgcaaaa
    10561 ttcattatct aattgaaaag aat
//
LOCUS       DAZFLD010000164        10481 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.165, whole genome shotgun sequence.
ACCESSION   DAZFLD010000164 DAZFLD010000000
VERSION     DAZFLD010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10481
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.165"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..234
                     /locus_tag="ACHKBL_003659"
     CDS             <1..234
                     /locus_tag="ACHKBL_003659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001303145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulator"
                     /protein_id="HFO5820476.1"
                     /translation="KASEPFTTDTGIVDRFFSLESNPRRDTPRSRNAVPPFSRRSREH
                     AAKNCREEYVQKAERRRRLIKAGLYIDEFENAL"
     gene            231..857
                     /locus_tag="ACHKBL_003660"
     CDS             231..857
                     /locus_tag="ACHKBL_003660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MT-A70 family methyltransferase"
                     /protein_id="HFO5820477.1"
                     /translation="MTKKYTLIYADPPWTFRDKATDGQRGASFKYPVMSLLDICRLPV
                     WELAADNCLLAMWWVPTQPLEALKVVEAWGFRLVTMKGLTWNKCGKRQTDKLVMGMGS
                     TTRANSEDCLFAVKGNLPERINAGIIQSFTAPRLDHSRKPDMAREKLVQLLGDVPRIE
                     LFARHTSHGFDVWGNQCGTPSIEMVPGIVKFLEKTNERKNDVDKGITS"
     gene            817..1029
                     /locus_tag="ACHKBL_003661"
     CDS             817..1029
                     /locus_tag="ACHKBL_003661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1382 family protein"
                     /protein_id="HFO5820478.1"
                     /translation="MSEKTTLTKASPVELRQCLEIANQLARSGIRFVPIPITADAELH
                     LFGEILSRKLDELEKLVEEADTSLTV"
     gene            1065..1427
                     /locus_tag="ACHKBL_003662"
     CDS             1065..1427
                     /locus_tag="ACHKBL_003662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000994797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HFO5820479.1"
                     /translation="MNAVLTELNKLGKASAESISKGLNIDLNDVIDTLWKLKNQGVVT
                     VKNGIWQAVAREVDKKPNIAPVQPVQPVQHNIIGDLLRKSRKEARRAGRKQKRWEGAC
                     KALQELNKYRDLINELSE"
     gene            1506..1688
                     /locus_tag="ACHKBL_003663"
     CDS             1506..1688
                     /locus_tag="ACHKBL_003663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO5820480.1"
                     /translation="MEVNLMTEKEVSDLLQKRRTALYNLRKKHGFPEPVLTHPARYSR
                     QAVEQWLKAGGINRAV"
     gene            complement(1672..2841)
                     /locus_tag="ACHKBL_003664"
     CDS             complement(1672..2841)
                     /locus_tag="ACHKBL_003664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO5820481.1"
                     /translation="MALSDTKLRGLYGKPYSGPAEITDGDGLSVRITPAGTITFQYRY
                     RWNGKPVRLTVGRYPSTSLKDARVIVGEMRALYMKGVNPKNYFAPSDGELTLKECLDQ
                     WWDKYVTDLKPNTQTLYRSVVYNTMYTQFEGSPVASIPVSAWARFFDKQESLNKKKAR
                     VLLLQLRSVINWCISRQLIPSCELLKLSVKNIGKKPDVGSRVLTYTELAKIWLALENS
                     KVVTSNKVLHQLLLLWGARLSELRLATASEFNMEDLVWTTPKEHSKMGNIIRRPVFTQ
                     VKPYIERLLNAGFDVLFPGQEIDKPIDRSSANLYMKKLREKIDIPEWRTHDFRRSLVT
                     NLSGEGIMPHVTEKMLGHELGGVMAVYNKHDWLSEQKDAYELYADKIFWHAKQLG"
     gene            3037..3111
                     /locus_tag="ACHKBL_003665"
     tRNA            3037..3111
                     /locus_tag="ACHKBL_003665"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3070..3072,aa:Arg,seq:cct)
     gene            3291..3473
                     /locus_tag="ACHKBL_003666"
     CDS             3291..3473
                     /locus_tag="ACHKBL_003666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001316510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HFO5820482.1"
                     /translation="MWRDSILHSAMHSLMNSQARDTRDTSLNDDNVLEISEPRIRSTF
                     ERLYQNMNEAFGLGDY"
     gene            complement(3553..4800)
                     /locus_tag="ACHKBL_003667"
     CDS             complement(3553..4800)
                     /locus_tag="ACHKBL_003667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO5820483.1"
                     /translation="MALNIPFRNAYYRFASSYSFLFFISWSLWWSLYAIWLKGHLGLT
                     GTELGTLYSVNQFTSILFMMFYGIVQDKLGLKKPLIWCMSFILVLTGPFMIYVYEPLL
                     QSNFSVGLILGALFFGLGYLAGCGLLDSFTEKMARNFHFEYGTARAWGSFGYAIGAFF
                     AGIFFSISPHINFWLVSLFGAVFMMINMRFKDKDHQCIAADAGGVKKEDFIAVFKDRN
                     FWVFVIFIVGTWSFYNIFDQQLFPVFYAGLFESHDVGTRLYGYLNSFQVVLEALCMAI
                     IPFFVNRVGPKNALLIGVVIMALRILSCALFVNPWIISLVKLLHAIEVPLCVISVFKY
                     SVANFDKRLSSTIFLIGFQIASSLGIVLLSTPTGILFDHAGYQTVFFAISGIVCLMLL
                     FGIFFLSKKREQIVMETPVPSAI"
     gene            complement(4872..5786)
                     /locus_tag="ACHKBL_003668"
     CDS             complement(4872..5786)
                     /locus_tag="ACHKBL_003668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoimidazole riboside kinase"
                     /protein_id="HFO5820484.1"
                     /translation="MSAKVWVLGDAVVDLLPESDGRLLPCPGGAPANVAVGIARLGGT
                     SGFIGRVGDDPFGALMQRTLLTEGVDITYLKQDEWHRTSTVLVDLNDQGERSFTFMVR
                     PSADLFLETTDLPCWRHGEWLHLCSIALSAEPSRTSAFTAMTAIRHAGGFVSFDPNIR
                     EDLWQDEHLLRLCLRQALQLADVVKLSEEEWRLISGKTQNDWDICALAKEYEIAMLLV
                     TKGAEGVVVCYRGQVHHFAGMSVNCVDSTGAGDAFVAGLLTGLSSTGLSTDEREMRRI
                     IDLAQRCGALAVTAKGAMTALPCRQELE"
     gene            6002..7435
                     /gene="cscA"
                     /locus_tag="ACHKBL_003669"
     CDS             6002..7435
                     /gene="cscA"
                     /locus_tag="ACHKBL_003669"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HFO5820485.1"
                     /translation="MTQSRLHAAQNALAKLHERRGNTFYPHFHLAPPAGWMNDPNGLI
                     WFNDRYHAFYQHHPMSEHWGPMHWGHATSDDMIHWQHEPIALAPGDENDKDGCFSGSA
                     VDDNGVLSLIYTGHVWLDGAGNDDAIREVQCLATSRDGIHFEKQGVILTPPEGIMHFR
                     DPKVWREADTWWMVVGAKDPGNTGQILLYRGSSLREWTFDRVLAHADAGESYMWECPD
                     FFSLGDQHYLMFSPQGMNAEGYSYRNRFQSGVIPGMWSPGRLFAQSGHFTELDSGHDF
                     YAPQSFLAKDGRRIVIGWMDMWESPMPSKREGWAGCMTLARELSESNGKLLQRPVHEA
                     ESLRQQHQSISPRTISNKYVLQENAQAVEIQLQWALKNSDAEHYGLQLGTGMRLYIDN
                     QSERLVLWRYYPHENLDGYRSIPLPQGDMLALRIFIDTSSVEVFINDGEAVMSSRIYP
                     QPEERELSLYASHGVAVLQHGALWQLG"
     gene            complement(7443..8438)
                     /locus_tag="ACHKBL_003670"
     CDS             complement(7443..8438)
                     /locus_tag="ACHKBL_003670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005088955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFO5820486.1"
                     /translation="MASLKDVARLAGVSMMTVSRVMHNAESVRPATRDRVLQAIQTLN
                     YVPDLSARKMRAQGRKPSTLAVLAQDTATTPFSVDILLAIEQTASEFGWNSFLINIFS
                     EDDAARAARQLLAHRPDGIIYTTMGLRHISLPESLYGENIVLANCVADDPALPSYIPD
                     DYTAQYESTQHLLAAGYRQPLCFWLPESALATGYRRQGFEQAWRDAGRDLAEVKQFHM
                     ATGDDHYTDLASLLNDHFKSGKPDFDVLICGNDRAAFVAYQVLLAKGVRIPQDVAVMG
                     FDNLVGVGHLFLPPLTTIQLPHDIIGREAALHIIEDREGGRVTRIPCPLLIRCST"
     gene            8681..8899
                     /locus_tag="ACHKBL_003671"
                     /pseudo
     CDS             8681..8899
                     /locus_tag="ACHKBL_003671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416866.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine transporter DsdX"
     gene            8917..10248
                     /gene="dsdA"
                     /locus_tag="ACHKBL_003672"
     CDS             8917..10248
                     /gene="dsdA"
                     /locus_tag="ACHKBL_003672"
                     /EC_number="4.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000426462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008721 - D-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046416 - D-amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
                     /protein_id="HFO5820487.1"
                     /translation="MENAKMNSLIAQYPLVKDLVALKETTWFNPGTTSLAEGLPYVGL
                     TEQDVQDAHARLSRFAPYLAKAFPETAATGGIIESELVAIPAMQKRLEKEYQQPISGQ
                     LLLKKDSHLPISGSIKARGGIYEVLAHAEKLALEAGLLTLEDDYSKLLSPEFKQFFSQ
                     YSIAVGSTGNLGLSIGIMSARIGFKVTVHMSADARAWKKAKLRSHGVTVVEYEQDYGV
                     AVEEGRKAAQSDPNCFFIDDENSRTLFLGYSVAGQRLKAQFAEQGRIVDADNPLFVYL
                     PCGVGGGPGGVAFGLKLAFGDHVHCFFAEPTHSPCMLLGVHTGLHDQISVQDIGIDNL
                     TAADGLAVGRASGFVGRAMERLLDGFYTLSDQTMYDMLGWLTQEEGIRLEPSALAGMA
                     GPQRVCASVSYQQMHGFSAEQLHNATHLVWATGGGMVPEEEMEQYLAKGRS"
     gene            complement(10353..>10481)
                     /locus_tag="ACHKBL_003673"
     CDS             complement(10353..>10481)
                     /locus_tag="ACHKBL_003673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter"
                     /protein_id="HFO5820488.1"
                     /translation="EITQQSLSISANEIFRMAAIAFILLTVLVWFAKPPFTAKGVG"
BASE COUNT         2956 a         2567 c         2426 g         2532 t
ORIGIN      
        1 aaggcttcag aaccattcac cacagacacc ggaatcgttg atcgcttttt ctccctcgaa
       61 tcaaatcctc gtcgtgacac acctcgctca cgcaatgccg ttcctccatt cagtcgcaga
      121 agccgtgaac acgcagcaaa aaactgtcgc gaggaatacg tgcaaaaggc cgaacgccgt
      181 cgccgactga ttaaagcagg actttacatt gatgagtttg aaaacgcgct atgacgaaaa
      241 aatacaccct catttacgca gatccaccat ggacattccg cgacaaagca accgatggtc
      301 aacgcggtgc aagttttaaa tatccggtca tgagtcttct ggatatctgc cgcctcccgg
      361 tatgggaact ggcagccgat aattgcctgt tggctatgtg gtgggtgcct acacaaccac
      421 ttgaagcatt gaaggttgta gaagcgtggg gctttcgtct ggtgacgatg aaaggattaa
      481 cctggaacaa atgcgggaaa agacagaccg acaagctggt catgggtatg ggtagcacca
      541 ctcgcgctaa cagcgaagac tgcctttttg cagtgaaagg aaatctgccc gaacgcatta
      601 acgccggaat aatccagtca ttcactgcac cacgccttga tcactcccgc aagccggata
      661 tggctcgaga aaaacttgtg caacttcttg gcgatgttcc ccggatagaa ctgttcgccc
      721 gccacacctc gcatggattt gatgtatggg gtaaccaatg cggcacacca tccattgaga
      781 tggttccggg tattgttaaa tttctggaga aaaccaatga gcgaaaaaac gacgttgaca
      841 aaggcatcac cagttgaatt aaggcagtgt ctggaaatcg caaatcaact tgccagaagt
      901 ggaatacgat ttgttccaat cccgattaca gcagatgcag aacttcatct gtttggtgaa
      961 attctttccc gaaagctgga tgaactggaa aagctggtag aagaagctga cacctcacta
     1021 accgtataac agccccaccg acattaaaat atcaggagaa aaaaatgaac gcagtgctca
     1081 cagaattgaa caaattagga aaagcatcag ccgaaagtat ttctaaaggt ctcaatattg
     1141 atttgaatga cgttattgac actctatgga agttaaaaaa ccagggggta gtaactgtaa
     1201 aaaatggcat ctggcaggca gttgcaaggg aagtggacaa aaaaccaaat atcgccccag
     1261 tgcagccagt gcagccagtg cagcacaaca ttataggtga cctgctacgt aaatcacgga
     1321 aagaagcgcg ccgcgccggg cggaaacaga aacgatggga gggtgcatgt aaggcgttgc
     1381 aagaactgaa taaataccgt gacttgatca acgaattgtc agagtgaacg tagcgggaat
     1441 ccacgtcctt tagggcgtgg atgatatcag gctgttgaaa tacatgtaga aaaggagcgc
     1501 atactatgga agtcaactta atgaccgaaa aagaggtttc tgatttgtta caaaaaaggc
     1561 gcacagcttt gtataatttg cgaaaaaaac acggattccc ggaaccagta cttactcatc
     1621 cggcgcgata tagtcgtcag gccgttgaac aatggcttaa agccggagga attaaccgag
     1681 ctgtttagcg tgccagaaaa ttttatcagc atacaactca tacgcatctt tctgttccga
     1741 caaccagtcg tgtttattat acacggccat aactccccca agttcatgcc ccagcatttt
     1801 ttcagtgacg tggggcataa ttccttcccc agataaattc gttaccagag aacgcctaaa
     1861 atcatgcgtt ctccattcag gaatatcaat tttctccctt aactttttca tgtacaaatt
     1921 agccgacgaa cgatcaatag gtttatctat ttcctgcccg gggaatagca catcaaaacc
     1981 cgcattaagc aatctctcga tataaggttt aacctgagtg aataccggac gccggataat
     2041 attccccatt tttgaatgct cttttggggt tgtccagacc agatcttcca tgttgaactc
     2101 actggctgtc gcgagacgta gctcagaaag tctggccccc catagcaata gcaattgatg
     2161 taacaccttg ttggaagtaa ctacttttga gttctccagc gccagccaaa tttttgccag
     2221 ttccgtatag gtaagtacac gactccctac atctggtttt ttcccaatat tcttaacact
     2281 aagcttgagt aattcgcatg atgggattaa ctggcggctg atacaccagt taataactga
     2341 acgcagttgt aacaacaaaa cacgagcctt tttcttgttg aggctttctt gtttgtcaaa
     2401 gaaacgagcc catgccgata ctgggatact ggcaactggc gagccttcaa actgtgtgta
     2461 catagtgttg tacacgacgg atctgtaaag cgtctgtgta ttgggtttca gatcagtaac
     2521 atacttatcc caccactgat ccagacattc ttttaatgtc agttcgccgt cactgggcgc
     2581 aaaataattt ttagggttaa ctcccttcat gtacaatgcg cgcatctcac cgacgataac
     2641 acgcgcgtcc ttcagtgaag tagacggata gcgcccgaca gtaagacgta ctggcttacc
     2701 attccagcga taacggtact gaaacgtgat agttcctgct ggagtaatcc gcacacttaa
     2761 cccgtcacca tcggtgattt cagcagggcc agagtagggt tttccgtaga ggcctcgtag
     2821 tttggtgtcg ctcagtgcca atgctttata tcctgtacac aagtttataa tgtattctgt
     2881 actcaatatg tacgcattag caagtgaaca aaacactttt caacgcaaaa gaagataatc
     2941 aaccatgaac aaaaacgaac ttacaccttg attttaaaac cataaagacg gtattatcag
     3001 tcatatggtg aacaaccaaa atcaatacgc aacaatgtcc tcttagttaa atggatataa
     3061 cgagcccctc ctaagggcta attgcaggtt cgattcctgc aggggacacc agataccctt
     3121 caaaccaaat ctaccttcac cccgtaaaag atgggtttgg cagcacactt gccctatatc
     3181 tactcatttt tactgcaaca ggttgaaatc tcagcactgt cagaaagcgc tgatgactaa
     3241 acagccctgg gccgggcgat gtaaccatca tacagaatcc tgatagcgaa atatggcgtg
     3301 actcgatact tcactctgca atgcattcct tgatgaattc gcaggcccgt gatacacggg
     3361 acacgtcgct gaatgacgac aatgtcctgg aaatcagcga accgcgtatc cggagtacat
     3421 ttgagcgact gtaccagaac atgaatgagg cgtttggatt aggtgattat tagcaggggt
     3481 aagcattttt gtattattat tttccggttg agggatatgg agatatcgac aacaaccgga
     3541 aaaagtttac gtctatattg ctgaaggtac aggcgtttcc ataactattt gctcgcgttt
     3601 tttactcagg aagaaaatgc caaatagcaa catcaggcag acaatacccg aaattgcgaa
     3661 gaaaactgtc tggtagcctg cgtggtcaaa gagtatccca gtcggcgttg aaagcagcac
     3721 aatcccaagc gaactggcaa tttgaaaacc aatcagaaag atcgtcgacg acaggcgctt
     3781 atcaaagttt gccacgctgt atttgaagac ggatatgaca caaagtggaa cctcaatggc
     3841 atgtaacagc ttcactaatg aaataatcca ggggttaacg aacagcgcgc aggaaaggat
     3901 acgcaacgcc ataatcacaa caccgataag taatgcattt tttggcccta cccgattcac
     3961 aaagaaagga ataatcgcca tgcacagcgc ttcgagtacc acctggaatg agttgagata
     4021 accatacagg cgcgttccta catcgtgtga ttcgaataaa cctgcataaa agacaggaaa
     4081 gagttgttga tcaaaaatgt tatagaaaga ccacgtcccc acaataaata tgacaaaaac
     4141 ccagaagttt cgatccttga aaactgcgat aaaatcctct ttttttaccc ctcccgcatc
     4201 cgccgctatg cactggtgat ccttatcttt aaaacgcatg ttgatcatca taaatacagc
     4261 gccaaatagc gagaccaacc agaagttgat atggggactg atactaaaaa atataccagc
     4321 aaagaacgcg ccaatagcat agccaaaaga tccccaggcg cgcgctgttc catattcgaa
     4381 atgaaaattt cgcgccattt tttcggtgaa gctgtcaagc aaaccgcatc ccgccagata
     4441 ccccaggcca aaaaagagcg cccccagaat tagacctaca gaaaaattgc tttgcagtaa
     4501 cggttcataa acgtaaatca taaacggtcc ggtcaagacc agaatgaaac tcatacacca
     4561 gatgagcggt ttcttcagac cgagtttatc ctgaacgatg ccgtagaaca tcataaatag
     4621 aatgctggta aactggttga ccgaataaag tgtacctaat tccgtccctg ttaatcctag
     4681 atgtcctttc agccaaatag cgtataacga ccaccacagc gaccaggaaa taaaaaagag
     4741 aaatgagtaa ctggatgcaa aacgatagta cgcatttctg aatggaatat tcagtgccat
     4801 aattacctgc ctgtcgttaa aaaattcatg tcctatttag agataagagc ggcctcgccg
     4861 tttacttctc actattccag ttcttgtcga catggcagcg ctgtcatcgc ccctttcgcc
     4921 gttactgcaa gcgctccgca acgttgagcg agatcgataa ttcgtcgcat ttctctttca
     4981 tctgtagata atcccgtaga ggacagacct gtgagtaacc cggcaacgaa cgcatctccc
     5041 gcccccgtgc tatcgacaca attcacagac attccagcaa aatggtgaac ttgtcctcga
     5101 taacagacca ccaccccttc tgcaccttta gtcaccaaca gcatggcgat ctcatactct
     5161 tttgccaggg cgcatatatc ccaatcgttc tgtgtttttc cactgataag tcgccattct
     5221 tcttccgaga gcttgacgac atccgccagt tgtagcgcct gccgcaaaca caagcggagc
     5281 aaatgctcgt cttgccatag atcttcacga atattgggat cgaagctgac aaaacctccg
     5341 gcatgccgga tcgccgtcat cgcagtaaat gcgctggtac gcgaaggctc ggcagacaac
     5401 gcaattgaac agagatgtaa ccattcgcca tgtcgccagc agggcaagtc tgtcgtctct
     5461 aaaaaaagat cggcactggg gcggaccata aacgtaaatg aacgttctcc ttgatcgttc
     5521 agatcgacaa gcaccgtgga tgtccggtgc cattcatctt gcttcagata cgtgatatcg
     5581 acaccctcag ttagcagcgt tctttgcatt aacgcaccaa aaggatcatc accgacccga
     5641 cctataaacc cacttgttcc gcctaatctg gcgattccca ccgcaacgtt agctggcgcg
     5701 ccgccaggac aaggtagtag ccgcccgtct gattctggca agagatctac gaccgcatcc
     5761 cctaaaaccc atactttggc tgacattttt ttcccttaaa ttcatctgac ttacgcatag
     5821 tgataaacct ctttttcgca aaatcgtcat ggatttacta aaacatgcat attcgatcac
     5881 aaaacgtcat agttaacgtt aacatttgtg atattcatcg catttatgaa agtaagggac
     5941 tttattttta taaaagttaa cgttaacaat tcacccaatt tgcttaacca ggatgataaa
     6001 aatgacgcaa tctcgattac atgcggcgca aaacgcacta gcaaaacttc acgagcgccg
     6061 aggtaacact ttctatcccc attttcacct cgcgcctccc gccgggtgga tgaacgatcc
     6121 aaacggccta atctggttta acgatcgtta tcacgcgttt tatcaacatc acccgatgag
     6181 cgaacactgg gggccaatgc actggggaca tgccaccagc gacgatatga tccactggca
     6241 gcatgagcct attgcgctag cgccaggaga cgagaatgac aaagacgggt gtttttcagg
     6301 tagtgctgtc gatgacaatg gtgtcctctc acttatctac accggacacg tctggctcga
     6361 tggtgcaggt aatgacgatg caattcgcga agtacaatgt ctggctacca gtcgggatgg
     6421 tattcatttc gagaaacagg gtgtgatcct cactccacca gaaggcatca tgcacttccg
     6481 cgatcctaaa gtgtggcgtg aagccgacac atggtggatg gtagtcgggg cgaaagatcc
     6541 aggcaacacg gggcagatcc tgctttatcg cggcagttca ttgcgtgaat ggactttcga
     6601 tcgcgtactg gcccacgctg atgcgggtga aagctatatg tgggaatgtc cggacttttt
     6661 cagccttggc gatcagcatt atctgatgtt ttccccgcag ggaatgaatg ccgagggata
     6721 cagttaccga aatcgctttc aaagtggcgt aatacccgga atgtggtcgc caggacgact
     6781 ttttgcacaa tccgggcatt ttactgaact tgatagcggg catgactttt atgcaccaca
     6841 aagcttttta gcgaaggatg gtcggcgtat tgttatcggc tggatggata tgtgggaatc
     6901 gccaatgccc tcaaaacgtg aaggctgggc aggctgcatg acgctggcgc gcgagctatc
     6961 agagagcaat ggcaaacttc tacaacgccc ggtacacgaa gctgagtcgt tacgccagca
     7021 gcatcaatct atctctcccc gcacaatcag caataaatat gttttgcagg aaaacgcgca
     7081 agcagttgag attcagctgc agtgggcgtt gaagaacagt gatgccgaac attacggatt
     7141 acagctcggc actggaatgc ggctgtatat tgataaccaa tctgagcgac ttgttttgtg
     7201 gcggtattac ccacacgaga atttagatgg ctaccgtagt attcccctcc cgcagggtga
     7261 catgctcgcc ctaaggatat ttatcgatac atcatccgtg gaagtattta ttaacgacgg
     7321 ggaggcggtg atgagtagcc gaatatatcc gcagccagaa gaacgggaac tgtcgctcta
     7381 tgcctcccac ggagtggctg tgctgcaaca tggagcactc tggcaactgg gttaacataa
     7441 tatcaggtgg aacaacggat caacagcggg caagggatcc gcgtcactct tcccccttca
     7501 cgatcttcaa taatatgcaa tgcagcttcc cgcccgataa tgtcatgtgg aagctgaatt
     7561 gtggtcagcg gcggtaaaaa cagatgcccg acgccaacca gattatcaaa gcccattacg
     7621 gcgacatcct gcgggatacg tacccccttc gccaggagaa cctgataagc gacaaaggct
     7681 gcgcgatcgt taccacatat cagaacatca aaatctggtt tgcccgattt gaagtggtca
     7741 ttgagtaaac ttgcgagatc ggtgtagtga tcatcacctg ttgccatgtg aaattgtttc
     7801 acctcagcca gatctcgtcc agcatcacgc caggcctgct caaatccctg ccgacgatac
     7861 cctgttgcca acgcactttc cggtagccag aagcataacg gttgacgata gcccgccgcg
     7921 agcaaatgct gtgttgattc atattgtgca gtgtaatcat cagggatata actgggtaac
     7981 gctgggtcat ccgccacaca gttcgccaat acaatatttt caccatacag agactcaggc
     8041 agcgagatat gtcgcagccc cattgtagta tagataatgc catccggacg gtgggcaagc
     8101 agctgacgtg ccgcgcgggc agcgtcatct tcagaaaaaa tattgattaa aaaactattc
     8161 cagccaaact cgctggcggt ttgctcaatg gcaagcagaa tatcaacaga gaaaggagtg
     8221 gtggccgtgt cctgcgccag cacggcgaga gtcgacggct tacgtccttg agcgcgcatc
     8281 ttacgggcgg aaagatcagg aacataattc agggtctgga ttgcctgcaa tacgcggtca
     8341 cgcgttgcag gacgcacaga ttctgcatta tgcatcaccc gggagactgt catcatcgac
     8401 actcccgcca ggcgtgcgac atcctttaat gaagccatac ccaagccgtt tgccgtaaaa
     8461 cgggcactgt agcagaaaca gacgtcactg gcgagatcca acgatattct caacgcccta
     8521 tcacccgaca cggcaataca ataaaaaata acaataattc ccggacaatt gtacccaatt
     8581 ccgcctctgt tctcgccaac gagtaaccat gtaggcaagt aggtaaaaca tgccaactgg
     8641 catcgccaac ctggagaaga aaatataaat atggatcatt gtgctgccgc tgtatcccga
     8701 catcagtccg gaaattattg cgattgctat cggttcaggt gcgattggct gcacgatcgt
     8761 tacggactcg cttttctggc tggtgaagca atattgcggc gctacgctca atgaaacatt
     8821 taaatactat acgacagcga catttatcgc ttcagtcgtc gctctggcgg gcacattcct
     8881 gctgtcattt atcatctaag cgcaaagaga cgtactatgg aaaacgctaa aatgaactcg
     8941 ctcatcgccc agtatccgtt ggtaaaggat ctggttgctc ttaaagaaac cacctggttt
     9001 aatcctggca cgacctcatt ggctgaaggt ttaccttatg ttggcctgac cgaacaggat
     9061 gttcaggacg cccatgcgcg cttatcccgt tttgcaccct atctggcaaa agcatttcct
     9121 gaaactgctg ctactggggg gattattgaa tcagaactgg ttgccattcc ggctatgcaa
     9181 aaacggctgg aaaaggaata tcaacaaccg atcagcgggc aactgctact gaaaaaagat
     9241 agccatttgc ccatttccgg ctccataaaa gcacgcggcg ggatttatga agtcctggca
     9301 catgcagaaa aactggctct ggaagcgggg ttgctgacgc ttgaagatga ctacagcaaa
     9361 ctgctttctc cggagtttaa acagttcttt agtcaataca gcattgctgt gggctcaacc
     9421 ggaaatctgg ggttatcaat cggcattatg agcgcccgca ttggctttaa ggtgacagta
     9481 catatgtctg ctgatgcccg ggcatggaaa aaagcgaaac tgcgcagcca tggcgttacg
     9541 gtcgtggaat atgagcaaga ttatggtgtt gcagtcgagg aaggacgtaa agcagcgcaa
     9601 tctgacccga actgtttctt tattgatgac gagaattccc gcacgttgtt ccttgggtat
     9661 tccgtcgctg gccagcgtct taaagcgcaa tttgccgagc aaggtcgtat tgtcgatgct
     9721 gataaccctc tgtttgtcta tctgccgtgt ggtgttggcg gtggtcctgg tggcgtcgca
     9781 ttcgggctta aactggcatt tggcgatcat gttcactgct tttttgccga accaacgcac
     9841 tccccttgta tgctgttagg cgtccataca ggattacacg atcagatttc tgttcaggat
     9901 attggtatcg ataaccttac cgcagcggat ggccttgcag ttggtcgcgc atcaggcttt
     9961 gtcgggcggg caatggagcg tttactggat ggcttctata cccttagcga tcaaaccatg
    10021 tatgacatgc ttggctggct gacgcaggaa gaaggtattc gtcttgaacc ttcggcactg
    10081 gcgggtatgg ccggacctca gcgcgtgtgt gcatcagtaa gttaccaaca gatgcacggt
    10141 ttcagcgcag aacaactgca taatgccact catctggtgt gggcgacggg aggtggaatg
    10201 gtgccggaag aagagatgga gcaatatctg gcaaaaggcc gttcataacg tttcaacgca
    10261 gcatcgccat cctttccctg ggtgagcgat gctgccgatg gcgcagactt aagatccccg
    10321 gtcttacctg ctataattct ctctttatac catcacccaa cgcctttcgc tgtaaacggc
    10381 ggtttcgcaa accaaaccaa taccgtaagt aagataaaag caatagccgc catacggaaa
    10441 atctcatttg cagaaattga aagtgactgc tgagttattt c
//
LOCUS       DAZFLD010000165        10342 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.166, whole genome shotgun sequence.
ACCESSION   DAZFLD010000165 DAZFLD010000000
VERSION     DAZFLD010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10342
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.166"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            84..2111
                     /gene="iha"
                     /locus_tag="ACHKBL_003674"
     CDS             84..2111
                     /gene="iha"
                     /locus_tag="ACHKBL_003674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014063836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional siderophore receptor/adhesin Iha"
                     /protein_id="HFO5820489.1"
                     /translation="MAAEDVMIVSASGYEKKLTNAAASVSVISQEELQSSQYHDLAEA
                     LRAVEGVDVESGTGKTGGLEISIRGMPASYTLILIDGVRQGGSGDVTPNGFSAMNTGF
                     MPPLAAIERIEVIRGPMSTLYGSDAMGGVVNIITRKNADKWLSSVNAGLNLQESNKWG
                     NSSQFNFWSSGPLVDDSVSLQIRGSTQQRQGSSVTSLSDTSATRIPYPTESQNYNLGA
                     RIDWKATEQDVLWFDMDTTRQRYDNQDGQLGSLTGGYDHTLRYERNKISAGYDHTFTF
                     GTWKSYLNWNETENKGRELVRSVLKSDKWGLAGKPRELKESNLILNSLLLTPLGESHL
                     LTVGGEYQSSSMKDGVVLASTGETFRQKSWSVFAEDEWHLTDVLALTAGSRYEHHDQF
                     GGHFSPRAYLVWDVTDAWTLKGGVTTGYKAPTMGQLHKGISGVSGQGRTNLLGNPELK
                     PEESVSYEAGVYYDNLSGLNANITGFITDFSNKIVSYSIDDNTNSYTNSGKARLHGVE
                     FAGTLPLWSEDVTLSLNYTWTQSEQRDGDNKGAPLSYTPEHMVNAKLNWQITEEVASW
                     LGARYRGKTPRFTRNYSSLSAVQKKVYDEKGEYLKAWTVVDAGLSWKMTDALTLNAAV
                     NNLLNKDYSDVSLYSAGKSSLYAGDYFQTGSSTTGYVIPERNYWMSLNYQF"
     gene            complement(2329..2479)
                     /locus_tag="ACHKBL_003675"
                     /pseudo
     CDS             complement(2329..2479)
                     /locus_tag="ACHKBL_003675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066419.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            2973..3215
                     /locus_tag="ACHKBL_003676"
     CDS             2973..3215
                     /locus_tag="ACHKBL_003676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5820490.1"
                     /translation="MKDEIHSTSINPDVVEQFLALMNTHCKSSEEALAMLSTLCYNLM
                     VTCGIHSMDSQMLNGHGISMQLQGIDIAERPGKNIH"
     gene            3506..3874
                     /locus_tag="ACHKBL_003677"
     CDS             3506..3874
                     /locus_tag="ACHKBL_003677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001456887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5820491.1"
                     /translation="MKDNNTTATPEAWKMRFLANGTSGTWFYTDDIAVTEAWLRAHPD
                     DVEVIPLCRIPPRQHSHEQHIEELRAALARMLHMHHMMLETTHYRENITDARCIREMH
                     EAPLQALNILARYPHKKDEV"
     gene            3878..4090
                     /locus_tag="ACHKBL_003678"
     CDS             3878..4090
                     /locus_tag="ACHKBL_003678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001622039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5820492.1"
                     /translation="MWLLIFWLSSPFNVSSGDARQPTLAVHSQTFHTERACRDALRAI
                     KKENVGEFSLRGVCVSDGNTKEAESS"
     gene            4087..4359
                     /locus_tag="ACHKBL_003679"
     CDS             4087..4359
                     /locus_tag="ACHKBL_003679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neurotensin receptor R8"
                     /protein_id="HFO5820493.1"
                     /translation="MTDITDRHTLRRMSWSELTTAAQEAELNRDYERARIVWSFALHV
                     ATTTINKNLAIAHIRRCDTLLHKSKTVPGNNPRGRSVCLRPQHPRR"
     gene            complement(4668..5499)
                     /locus_tag="ACHKBL_003680"
                     /pseudo
     CDS             complement(4668..5499)
                     /locus_tag="ACHKBL_003680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000555401.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            5557..5745
                     /locus_tag="ACHKBL_003681"
                     /pseudo
     CDS             5557..5745
                     /locus_tag="ACHKBL_003681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(5814..7040)
                     /locus_tag="ACHKBL_003682"
     CDS             complement(5814..7040)
                     /locus_tag="ACHKBL_003682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO5820494.1"
                     /translation="MKDMQAMLPQTFYIKPGQFIWSYLGTFLFMLGGCIENSWLSAWL
                     NTQGFDQSQIGQIFAAYGIVVAVTSWLSGVCVDMFGPRKVMLAGFSVYLIASIAFLNF
                     AIPSHDFSTILISYMLRGVGYPLVCYSFLVRLTIQLDNHQQGIGTSLFWVVYNLGFTI
                     IGPVVAASLIPVLGHINVMWAGIGVALLGGIFMLVFERNAFILKPRTTSVFKELSAGI
                     SIMYQRPRIGLAVIVKTINGLGTYGFVVVLPLFLLDKQFTLEEWASIWGITFISNQIF
                     NIIFGWMGDKIGFRRTIQIFGSLLTGFATLIVYWVPIVWGHNYVAFMLAMCLWGAGLA
                     GFVPMTPLVPMMAPDKKGAANSAVNFGSGLGNFVGPALVSILTGFGTGVVMYTMAGLY
                     LFSGILVQFLKVPGEK"
     gene            complement(7113..8135)
                     /gene="iolG2"
                     /locus_tag="ACHKBL_003683"
     CDS             complement(7113..8135)
                     /gene="iolG2"
                     /locus_tag="ACHKBL_003683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-chiro-inositol-2-dehydrogenase IolG2"
                     /protein_id="HFO5820495.1"
                     /translation="MQKLRCGIIGLGRVGKMHVENMYLLPQLDIVCAADSFIEEMSDW
                     LSSVNITSGYKDYQEMLQRDDIEAVFIFTSTDMHEEIVTAAVQAGKHIFCEKPLSMRE
                     DEKASLAVLRAVKEKGVKLQIAFNRRFDPQFREVYELVRRGKIGQPQMIKITSRDPEL
                     LPHDLIKRIGGLIFDFTMHDFDMARFIMQDEVSEVYVKGNTLIDPTLKNIDDVDTLVV
                     MLTFRNGGYALIDNSRRAVYGYDQRVEVFGSAGMAYADNVSESAVKVYNSQNCIMKNP
                     LPDFALRYREAYRTEILHFIDSVLNHTPIVCTGEDALLAQRIAIAAKQSLKSGLPVKI
                     KTDIQL"
     gene            complement(8308..10248)
                     /gene="iolD"
                     /locus_tag="ACHKBL_003684"
     CDS             complement(8308..10248)
                     /gene="iolD"
                     /locus_tag="ACHKBL_003684"
                     /EC_number="3.7.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_453702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016823 - hydrolase activity, acting on
                     acid carbon-carbon bonds, in ketonic substances [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3D-(3,5/4)-trihydroxycyclohexane-1,2-dione
                     acylhydrolase (decyclizing)"
                     /protein_id="HFO5820496.1"
                     /translation="MKTIRLTMAQALVRFLDNQYIEVDGREIKFVKGIFAIFGHGNVV
                     GLGQALEEECGQLSVHQGRNEQGMAHIATGFARQMRRQQIYACTSSVGPGAANMITAA
                     ATATANRIPLLLLPGDVYASRQPDPVLQQIEQEHDLTLSTNDAFRAVSRYWDRIIRPE
                     QLMSACISAMRVLTDPADTGAVTLCLPQDVQGEAWDYPDYFFARRVYRLERHVPTELM
                     LNDAVALILRHQRPIIICGGGVKYSQSEQELLRFAEQYHLPIAETQAGKGALSSAHPL
                     NVGGIGETGSLAANLLAQEADLIIGVGTRYTDFTTSSKWIFQNPDVRYLNINVSRFDV
                     FKLDGVQLQADARVALTQLCERLAPEGYISQWGKTIHRVRSQYLAEVDRVYAAEYSGK
                     DFKPEIVDHMDTQKVFEEFNEITHSWLTQTRVLGVLNRMLPENALVVAAAGSLPGDLQ
                     RIWQSRGDNDYHVEYGYSCMGYEVNAALGAKLAQPEREVYSFVGDGSFMMLHSELVTS
                     VQMGKKINVILLDNMTNGCINNLQMEHGMDSFFTEFRFHKQDNGRQEGGLIPVDFARI
                     AEGYGCKSYRVTSIGQLLEALEDARKQTVSTLIDIKVLPKTMAHKYLSWWRVGGARVS
                     RSERIQAVARMLDEHIGQARQY"
BASE COUNT         2867 a         2630 c         2320 g         2525 t
ORIGIN      
        1 taacatgatc ggagattagt aatatgcgaa taaccactct ggcttccgtt gtcattccct
       61 gtctcgggtt ttcagccagc agcatggcag cagaggatgt gatgattgtc tcggcatccg
      121 gctatgagaa aaaactgacc aacgcagccg ccagtgtttc cgtaatcagt caggaggaat
      181 tgcagtccag ccagtaccac gatctggcgg aggcactgag agctgtggag ggtgtggatg
      241 ttgaaagtgg tacgggtaaa accggtgggc tggaaatcag catccgtgga atgcccgcca
      301 gttacacgct gatactgatt gatggtgtcc gtcagggcgg gagcggggac gtgacgccta
      361 acggtttttc tgccatgaat acagggttca tgcctcctct ggccgcgatt gagcgtattg
      421 aggttatcag ggggccgatg tccacactgt atggctctga tgcgatgggt ggtgtggtga
      481 atatcattac cagaaagaat gcagacaaat ggctctcttc cgtcaacgcc gggctgaatc
      541 tgcaggagag taacaaatgg ggcaacagca gccagtttaa tttctggagc agtggccctc
      601 ttgtggatga ttctgtcagt ctgcagatcc gcggcagcac acagcagcgt cagggctcat
      661 cggtcacatc actgagcgac acatcagcca cgcgtattcc ttatcccacg gagtcacaga
      721 attataatct tggtgcgcgt attgactgga aggctacgga gcaggatgtg ctctggtttg
      781 atatggatac cacccgacag cgttacgata accaggatgg tcaactgggt agcctgacag
      841 ggggatatga ccatactctg cgctatgagc gaaacaaaat ttcggctggc tatgatcata
      901 ctttcacctt cggtacctgg aaatcgtatc tgaactggaa cgagacggaa aataaaggtc
      961 gtgagcttgt acgtagtgtt ctgaagagcg ataaatgggg gcttgccggt aaaccgcggg
     1021 agcttaagga atctaacctt atcctgaatt cattgctgct tacccctctg ggagaatctc
     1081 atctgcttac tgtgggggga gagtatcaga gttcgtccat gaaagatggg gttgtccttg
     1141 ccagcacagg tgaaactttc cgacagaaaa gctggtcagt atttgctgag gatgagtggc
     1201 atctcacgga tgtacttgcg ctgactgcag gcagtcgcta tgaacatcat gatcaattcg
     1261 ggggacactt cagtccgcgt gcatatctgg tctgggacgt gaccgatgcc tggacgctga
     1321 aaggcggtgt gaccacggga tataaagccc ccaccatggg acagttacat aaggggatca
     1381 gcggtgtatc cgggcaggga cgtactaatc tactcggtaa ccccgaactg aagccggaag
     1441 agagtgtaag ttatgaggcg ggggtatatt acgataacct ctccggtctg aatgccaata
     1501 taacaggctt tataacagac ttctccaaca agattgtctc ttattccatt gatgataaca
     1561 ccaatagcta tacaaacagc ggaaaggccc gcttgcatgg tgtggaattc gccggcacat
     1621 tgccgctgtg gtcagaggat gtcactctgt cgttgaatta cacctggacc caaagtgaac
     1681 aacgtgatgg tgataacaaa ggtgcaccgc tgagttatac ccctgaacac atggtgaatg
     1741 cgaaactgaa ctggcagatc accgaagagg tggcatcatg gctgggtgcc cgttatcgcg
     1801 ggaaaacacc acgtttcacc cggaattatt cgtcactgag cgctgtacag aagaaagtgt
     1861 atgatgagaa aggagaatac ctgaaagcct ggacggtggt ggatgcaggt ctgtcgtgga
     1921 agatgacgga tgccctgacg ctgaatgctg cggtgaataa cctgctcaac aaggattaca
     1981 gtgacgtgag cctgtacagt gccggtaaga gttcgctgta tgccggtgat tacttccaga
     2041 cgggatcatc aacaacagga tatgtgatac ctgagcgaaa ttactggatg tcgctgaact
     2101 atcagttctg ataataacaa aacgctatca ctgacggtag aatacgttgc cactgcaact
     2161 cctggcggaa cagtggcaac gtattaggtt aagtgcattt ccgatccgct aatgagattt
     2221 cgttaccaac aactaatatc gtcacaggaa atgcacggat tatttttaac ttatcattta
     2281 catacttgtc cagagtgtaa gcgcaccgtg aaggacgtgg ggtaaaaatt agtttacaga
     2341 gagagtgacg ttccagggca acaactcttt catgcggttg gcaggccagg tgttgattac
     2401 actgatcacg tggcgtagcc acgtttccgg ctcgattccg ttaagttttg cagctaccga
     2461 tcaggctgta catcactgcc gcactatcgc tcgtcatctc aaagtccggt ctcgtcagca
     2521 ggaaggtatc attctctccc gccatttttc cgggggcccg gtcagataag tccctttgtc
     2581 tatcgctgac tcctggctca taacccggtt agcagaatgc agggtcacca cctcgccacg
     2641 accgaatccg gataagtcaa ttgcaccttc tcaatcgcca ttttgtcaca attaagtttg
     2701 ataaagcagg tcacctctgc actgaatctg ctccagtgcc tcattggtct ccgtcccccc
     2761 ctttaaactg ccggataaga ctgctgcaca gcgccttccc cggaatcaaa tcactttccc
     2821 tgtccgccac aatccgcttc atgctacctc gtattttgtt tcagagaccg gaatctgcca
     2881 gaaagtgact acgcgtgcac atatcggttt tctgggttac atgcatcgtg ttattttctg
     2941 aaatgcaaaa taataaaaaa caaggacaca taatgaagga tgaaatacac agtaccagca
     3001 tcaacccgga tgttgttgag cagtttctgg cccttatgaa cacacattgt aaatcctcag
     3061 aagaggcgct cgccatgttg agtacgcttt gttacaacct gatggtgaca tgtggcatcc
     3121 attccatgga cagccagatg ctcaatgggc atgggatatc catgcaactt caggggattg
     3181 atattgccga aaggccaggt aagaatattc actaacctga acagggcctg aaaacgctgt
     3241 cttttcaggc ctctgcaggc aaaaactgat gacaacaaaa tttctgcgtt tgtaccccac
     3301 cgatgaaaaa gacaaaaact cacaccgggc ttctagccag taaagataaa acccgacgtg
     3361 tcagcctcta cgaaacgcca acggcatggt gtatcagggg gcaggagtgc tacagcaaaa
     3421 gtacaggacg ccgctgtggc agccacgaca gtctgagcag actgcgccta gacagcatta
     3481 agcctgttga atgagtggtg gcaccatgaa agataacaac acaaccgcca ccccagaagc
     3541 ctggaaaatg cgttttctgg ccaacgggac atccggcaca tggttttaca ctgacgacat
     3601 tgcagtcact gaggcgtggc ttcgcgcgca cccggatgat gtggaggtta tccctctctg
     3661 ccggatacca ccccgtcagc attcccatga acaacatatt gaagaactcc gcgcagccct
     3721 ggcccggatg ctgcatatgc atcatatgat gctggaaacc acgcattaca gggaaaacat
     3781 caccgatgcc cgttgtatcc gtgaaatgca tgaagctcca ttacaggcgc tcaatattct
     3841 ggccagatac ccgcataaaa aggacgaggt gtgacgcatg tggctactga ttttctggct
     3901 ttcatctccc tttaatgtct cttccggtga tgcccggcag cccacactgg cggtgcacag
     3961 ccagacattt cataccgaac gggcctgtcg cgatgcattg cgcgccatta aaaaggagaa
     4021 tgttggcgaa ttttctctgc ggggcgtttg tgtttctgac ggaaacacga aggaggcaga
     4081 gagctcatga cggatattac tgacagacat acacttcgcc gcatgtcctg gtcggaactt
     4141 accacggcag cgcaggaagc agaattaaac cgtgattacg agcgtgccag aatagtgtgg
     4201 tcattcgcct tacatgttgc aacaaccacg attaacaaaa atctggccat agctcatatt
     4261 cgacgctgcg atactttgtt gcataaatca aaaacagtac ccggaaacaa cccgagaggc
     4321 cggtcagtgt gcctgagacc tcaacatccc cggagatgac tgacattccg gactgacgca
     4381 gttgctgcat atctgtggca atacgttgta tccagccaga caggctatcc cgcgtagtga
     4441 caattaaccc gctttcctgc accagcttca gccgggccgt cggggatttt accagccgga
     4501 aatatcagta aaacaacata tcattatgcc atggtgtacg cggccagtgt atgcttactt
     4561 aaggaatgag ggataaattg gatattatta cttcactttg cttttttatg agattcatca
     4621 cataagtggg gttttactgt taaataatct gaatttattt gatatccgta aagcagatgt
     4681 ctcagttcac tgtttccaac ttttgttatt cctctggggc gaatgctgtt tccgctggaa
     4741 tattcacctg gcaccagtcc cagaaaagcc atgagctgtt caggattgga aaatcgcgaa
     4801 aaatcaccca gttcagcaac cagggttatt gcgattagct gaccaacacc tttaagagcc
     4861 tgcaactggc agaccagatt gcacagagat cactcaggta acaggcgaga tatttcctga
     4921 tcgagttgaa gtatgcgatc ttcaatctgc tccagattct gacaataatg ttgaaagcaa
     4981 tctgttgaga tagcagacca aatgattgat taactaacca ggttcgatgt ttgccggttc
     5041 agtgcttcct ggcataacgt ttgtctgttc gtgtcacgtt ttgcagcggc acgggctctg
     5101 attaaatccc gcatagcttc atgagtcagc tcagggagcc agactggggg aacctcacca
     5161 tccctcagta acctggcgag agaaattacg tcccgatggt catttttgat cctgtctgcc
     5221 ggtgatttgg gtatacggga agggggcaat aaccaggcat tttatattca tcactgtgag
     5281 ttgatgatat agcccaaacc cacagggacc accttcatag caggaaagaa tgtttccgta
     5341 aggtccctgc aatttctgac ataatcgatg tacagccaag gcttcgttgt taatcgtacc
     5401 ataataacga acttcgccaa gccattcagg tgtagcaatc gccaaggttt ctttatgaac
     5461 atccagcccg acgtaagctg caatattttc cgaatccatg atgacctctg gagtatccca
     5521 ccaatgctat agtgagtata taaaggtcat atcatctacg tgttcttcgg caacgatcac
     5581 agtggagagc gtggagcgct actgtacggg ctgattggca cctgccgact gaacagtatc
     5641 gatccggaag cgtatctgcg ccatattctg agcgtactgc cggaatggcc ttccaaccgt
     5701 gttgacgaac tgctgccatg gaacgtagtt ctcaccgata aataagcgtc aatacggcat
     5761 tcgattaatg cacttacagc taaatggagt ttttgaacaa tatttgacgt tatttatttc
     5821 tcaccaggaa ctttaagaaa ctgaaccagg atgccactaa ataaatagag tcctgccata
     5881 gtatacataa ccacgcctgt accaaaacct gtcagaatgg ataccaacgc aggtccgaca
     5941 aaatttccca ggccagaacc gaaattcacc gctgagtttg ctgccccctt cttatccggt
     6001 gccatcatcg gtaccagcgg cgtcatcggc acaaatcccg ccagccccgc tccccacagg
     6061 cacattgcca gcataaaagc aacgtaatta tgcccccaca ctatcggcac ccagtagaca
     6121 atcagcgtgg caaacccggt cagaagactg ccaaaaatct ggatagtacg gcgaaaacct
     6181 attttatccc ccatccagcc gaaaataatg ttgaaaatct gattagaaat aaaggttata
     6241 ccccagatac tcgcccactc ctcaagggta aactgtttat ccagcaaaaa cagcggcaac
     6301 accactacaa aaccataggt gccgagtcca ttgattgttt taacaatcac tgccagacca
     6361 attcggggtc gttgatacat aatggaaata cctgccgaca actctttaaa gactgacgta
     6421 gttcttggct tcagaatgaa cgcattgcgc tcaaagacca gcatgaaaat ccccccaagc
     6481 agtgcaaccc caatcccggc ccacataaca ttaatatgcc caaggacagg aattaacgaa
     6541 gcagctacta ccggaccaat gatggtaaaa cctaagttat atactaccca aaacagcgac
     6601 gttccaattc cctgttgatg attatcgagt tgaatggtca gtcggacaag aaaggaatag
     6661 cagaccaacg gataacctac tccccgcagc atatagctaa tgagaattgt actaaaatca
     6721 tggctgggaa tcgcaaaatt aagaaacgcg atagaagcga tcaggtaaac ggagaaacca
     6781 gcaagcataa cctttctggg gccaaacata tccacacaga caccggataa ccaggaagtt
     6841 acggcaacca caattccgta ggcggcaaat atctgtccga tctgagactg atcaaatccc
     6901 tgagtattca gccaggcaga tagccaacta ttttcaatac agccgcccaa cataaataaa
     6961 aatgttccca ggtagctcca gataaactga cctggcttta tataaaatgt ctgaggcaac
     7021 attgcctgca tatctttcat tttataaccc tcagtacgga taatctctcg ctcgtaatag
     7081 tgcgagagac ccttcgctat ttacaaatgc aactaaagtt gaatatcagt ttttatcttc
     7141 actggcagtc cactttttaa tgattgtttt gcagcaatgg caatacgctg cgccaataat
     7201 gcatcttcac cagtacagac aatgggtgta tgattcagaa cagaatcaat aaagtgtaat
     7261 atttccgttc tataagcttc acgatagcgt agcgcaaaat caggcaaagg atttttcatg
     7321 atacagttct gactgttata taccttcacc gccgattcac tgacattgtc ggcatacgcc
     7381 atccctgctg aaccaaagac ctcaacccgt tggtcgtagc cataaaccgc ccgacgacta
     7441 ttatcaatta gcgcataacc accgtttctg aaggtcagca tcaccacgag agtatctacg
     7501 tcatcaatat ttttcagagt aggatcaatc aatgtattgc ctttaacata aacctcgcta
     7561 acttcatcct gcataataaa gcgagccata tcaaagtcat gcatagtaaa atcaaatatg
     7621 agtccaccta tcctcttgat caaatcgtgc ggcaacaact ctggatcacg tgaggtgatc
     7681 tttatcatct gaggctgacc aatcttgcct ctgcgcacca gttcatacac ctcccgaaac
     7741 tggggatcaa aacgacgatt aaaagcaatc tgcagtttga ctcctttctc tttaaccgcc
     7801 cttagcaccg caagggaagc tttctcatct tctctcatgc tgagaggttt ttcgcagaaa
     7861 atatgtttac ccgcctggac tgctgccgta acgatctctt cgtgcatatc tgtcgaggtg
     7921 aaaataaaca ccgcctcaat atcatcacgc tgtaacattt cctgataatc tttatatcct
     7981 gaggttatat ttaccgaact cagccagtcg ctcatttcct caataaaact gtcagcagca
     8041 cagacaatat ctaattgagg aagtaaatac atattctcaa catgcatttt ccctacccgg
     8101 ccaagaccaa taatcccaca tcgaagcttt tgcatttata cctcgtcata tcatgatata
     8161 gtaaatataa aacgacaaaa gaaaatcccc tttatccaac acggtacgaa agactttatt
     8221 ttgtagtttc aacgaacaaa tacttttacc gtaaaaaacg tcaggttgca gaaaggcgcc
     8281 ctcctgcaac cttaaaaact gaattaatta gtactgtcgc gcttgtccaa tatgctcatc
     8341 aagcatacga gctactgcct ggatgcgttc gctacgcgac acgcgagcgc caccaacacg
     8401 ccaccagctc agatatttat gcgccattgt tttaggcagt actttaatgt ctatcaacgt
     8461 actcaccgtc tgtttacgtg catcctccag agcttcaagc agttgtccaa tgctggtgac
     8521 gcgatagctt ttacagccat atccttcggc aatccgggca aaatccacag gaatgagtcc
     8581 cccctcctga cgaccgttat cctgtttatg gaaacgaaac tcggtaaaga aactgtccat
     8641 accgtgttcc atttgaagat tatttataca accattagtc atgttatcca gcaaaataac
     8701 attaattttc ttccccatct ggaccgaagt taccagctca gaatgcagca tcataaatga
     8761 gccgtcgcca acgaagctat atacctcccg ctccggttgc gccaacttcg cccccaatgc
     8821 ggcattgacc tcatagccca tacaggaata gccatattct acgtggtaat cattatcgcc
     8881 acggctttgc cagatgcgct gcagatcgcc tggcagactt cccgccgcag caaccaccag
     8941 cgcgttttct ggcagcattc gatttagtac tccaagaacg cgcgtctgtg tcagccatga
     9001 atgagtaatt tcattgaact cttcaaacac tttttgagta tccatatgat ccacaatttc
     9061 cggtttgaaa tccttaccgc tgtattccgc tgcatagacg cgatccactt ccgccagata
     9121 ttgagaacga acacgatgga tcgttttccc ccattgtgat atgtaacctt ccggagccag
     9181 tcgttcgcaa agctgcgtca gggcaacacg ggcatcagcc tgcaattgca ctccatccag
     9241 tttgaagacg tcaaaacggc tgacgtttat attcagatag cgcacatcag gattctggaa
     9301 aatccattta gaagaggttg tgaaatcggt ataacgcgta cccacaccaa taatcagatc
     9361 ggcctcctga gccagcaaat tcgccgccag cgatccggtt tcaccaatcc caccaacatt
     9421 cagcggatgc gcagaactga gcgcaccttt accagcctgc gtttcagcaa tcggcagatg
     9481 atattgttcg gcaaatcgca gcagttcctg ttcagactgc gaatacttca cacccccacc
     9541 gcaaataata attggtctct ggtggcgaag aataagagca actgcatcat tcagcattag
     9601 ctctgttggc acgtggcgct caagacgata gacccgacgt gcaaaaaaat agtctggata
     9661 atcccaggct tctccctgaa catcctgcgg caaacaaagc gtcacagctc ccgtatcagc
     9721 cggatcggta agcacccgca tcgcgctaat gcaggcgctc attagctgtt ccggacggat
     9781 aatgcgatcc caatagcgac tcactgcacg aaaagcatca ttggtgctca gcgtcaggtc
     9841 atgctcctgt tcaatctgct gcaacacagg atcgggttga cgcgatgcat acacatctcc
     9901 tggtagcaga agcaacggga tacggttcgc cgtcgcagtc gccgccgccg tgatcatatt
     9961 agcggcacca ggccctacgg aggaggtgca ggcataaatc tgctgacgac gcatctgacg
    10021 cgcaaatcct gttgctatat gtgccatacc ctgttcattg cgtccttgat gaacgctaag
    10081 ttgaccacac tcttcttcca gagcctgccc taatcccacg acattcccat ggccaaaaat
    10141 tgcgaaaata ccttttacaa acttgatttc gcgaccgtcc acttcaatgt actgattatc
    10201 aagaaagcgc accaaagcct gcgccatagt caacctgatt gttttcatgc actccaccta
    10261 tcttatcaac cgtttgactg tcgttaccta cgtacgcact gctacagccc cgggcataaa
    10321 acactcacca caagaaaaaa at
//
LOCUS       DAZFLD010000166        10305 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.167, whole genome shotgun sequence.
ACCESSION   DAZFLD010000166 DAZFLD010000000
VERSION     DAZFLD010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10305
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.167"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            172..1080
                     /locus_tag="ACHKBL_003685"
     CDS             172..1080
                     /locus_tag="ACHKBL_003685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005743 - mitochondrial inner membrane
                     [Evidence IEA];
                     GO_component: GO:0035632 - mitochondrial prohibitin
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HFO5820497.1"
                     /translation="MKAPVSITSFRPQKSIAIVIGVLAVVVLPFLSYYTVNEGERGIL
                     LRYGKIVKVAEPGLGFKIPFMESVEKISTRNQAVVYQGLQAYSRDQQPAQMTVSVSFH
                     IKPSEAGAVYTTYNTIEALKDRLIVRQLPTQLENVFGQYTAISAVQDRTKLVQDLQNA
                     MRKAVVGPVVIDGVQIENIDFSDAYEKSIEDRMKAEVAIATRKQNLETEKIQAQIAVT
                     QAQAEADSKLAAAKAEAETIRVRGAAEAETIRLKSAAEAEAIRLRGEALRDNPGLVAL
                     TTAERWDGKLPDTMIPGSTVPFISTK"
     gene            complement(1126..3117)
                     /gene="tkt"
                     /locus_tag="ACHKBL_003686"
     CDS             complement(1126..3117)
                     /gene="tkt"
                     /locus_tag="ACHKBL_003686"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFO5820498.1"
                     /translation="MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDF
                     LKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPE
                     VGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
                     GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDG
                     HDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQ
                     LGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFAAYAKAYPQEAAEFTRRMKGEMP
                     SDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSG
                     SKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAAL
                     MKQRQVMVYTHDSIGLGEDGPTHQPVEQVASLRVTPNMSTWRPCDQVESAVAWKYGVE
                     RQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVA
                     AYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVG
                     LNGAIVGMTTFGESAPAEQLFEEFGFTVDNVVAKAKALL"
     gene            3395..4153
                     /gene="loiP"
                     /locus_tag="ACHKBL_003687"
                     /gene_synonym="yggG"
     CDS             3395..4153
                     /gene="loiP"
                     /locus_tag="ACHKBL_003687"
                     /gene_synonym="yggG"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease LoiP"
                     /protein_id="HFO5820499.1"
                     /translation="MKIRALLVAMSVATVLTGCQNMDSNGLLSSGAEAFQAYSLSDAQ
                     VKTLSDQACQEMDSKATIAPANSEYAKRLTTIANALGNNINGQPVNYKVYMAKDVNAF
                     AMANGCIRVYSGLMDMMTDNEVEAVIGHEMGHVALGHVKKGMQVALGTNAVRVAAASA
                     GGIVGSLSQSQLGDLGEKLVNSQFSQRQEAEADDYSYDLLRQRGISPAGLATSFEKLA
                     KLEEGRQSSMFDDHPASAERAQHIRDRMSADGVK"
     gene            complement(4201..5121)
                     /gene="speB"
                     /locus_tag="ACHKBL_003688"
     CDS             complement(4201..5121)
                     /gene="speB"
                     /locus_tag="ACHKBL_003688"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HFO5820500.1"
                     /translation="MSTLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGVPFDM
                     ATSGRAGGRHGPAAIRQVSTNLAWEHNRFPWNFDMRERLNVVDCGDLVYAFGDAREMS
                     EKLQAHAEKLLAAGKRMLSFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCE
                     FDHGTMFYTAPKEGLIDPNHSVQIGIRTEFDKDNGFTVLDACQVNDRSVDDVIAQVKQ
                     IVGDMPVYLTFDIDCLDPAFAPGTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVA
                     PAYDQSEITALAAATLALEMLYIQAAKKGE"
     gene            complement(5257..5988)
                     /locus_tag="ACHKBL_003689"
     CDS             complement(5257..5988)
                     /locus_tag="ACHKBL_003689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5820501.1"
                     /translation="MKKWKVRSALIALAVLLAGCSSNAQYNSSASGNVGTAWGGDVHS
                     SVQGVSAERVWRDPAEMIVISYSTTVPSGYDRVYSIRINELEYAIRDGNFNSLPITRV
                     YDSSNNEPRYIVHARVGMNYQLYIRNYSRNTNYEIVATVDGLDVLNGKQGSLNNNGYI
                     VNAGDSLAIKGFRKDKHTEAAFQFANVADSYAANSAQGDVRNTGVIGFAAFELQGPAQ
                     NALPPCSGQAFPADNNGYAPPPCRK"
     gene            complement(6134..8110)
                     /gene="speA"
                     /locus_tag="ACHKBL_003690"
     CDS             complement(6134..8110)
                     /gene="speA"
                     /locus_tag="ACHKBL_003690"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HFO5820502.1"
                     /translation="MSDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWW
                     GNNYYDVNELGHISVCPDPDVPEARVDLAQLVKTREAQGQRLPALFCFPQILQHRLRS
                     INAAFKRARESYGYNGDYFLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLA
                     HAGMTRSVIVCNGYKDREYIRLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPR
                     LGVRARLASQGSGKWQSSGGEKSKFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQ
                     MANIRDIATGVRESARFYVELHKLGVNIQCFDVGGGLGVDYEGTRSQSDCSVNYGLNE
                     YANNIIWAIGDACEENGLPHPTVITESGRAVTAHHTVLVSNIIGVERNEYTVPTAPAE
                     DAPRALQSMWETWQEMHEPGTRRSLREWLHDSQMDLHDIHIGYSSGTFSLQERAWAEQ
                     LYLSMCHEVQKQLDPQNRAHRPIIDELQERMADKMYVNFSLFQSMPDAWGIDQLFPVL
                     PLEGLDQVPERRAVLLDITCDSDGAIDHYIDGDGIATTMPMPEYDPENPPMLGFFMVG
                     AYQEILGNMHNLFGDTEAVDVFVFPDGSVEVELSDEGDTVADMLQYVQLDPKTLLTQF
                     RDQVKKTDLDAELQQQFLEEFEAGLYGYTYLEDE"
     gene            complement(8119..8250)
                     /gene="yqgB"
                     /locus_tag="ACHKBL_003691"
     CDS             complement(8119..8250)
                     /gene="yqgB"
                     /locus_tag="ACHKBL_003691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response protein YqgB"
                     /protein_id="HFO5820503.1"
                     /translation="MKKKPVAQLERQHSLLENPCAYGLLSQFQAAIVVNCFTLNKII"
     gene            complement(8344..8487)
                     /gene="yqgG"
                     /locus_tag="ACHKBL_003692"
     CDS             complement(8344..8487)
                     /gene="yqgG"
                     /locus_tag="ACHKBL_003692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgG"
                     /protein_id="HFO5820504.1"
                     /translation="MNRCLLLNLSHRSGEDSFPALCISALHTCRCYTHLGASQDSRAG
                     YSY"
     gene            complement(8598..8849)
                     /gene="yqgD"
                     /locus_tag="ACHKBL_003693"
     CDS             complement(8598..8849)
                     /gene="yqgD"
                     /locus_tag="ACHKBL_003693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgD"
                     /protein_id="HFO5820505.1"
                     /translation="MVADPTTTLQVKNTGSLSVNRYGWINIWMAILGQFFTRFPLFFE
                     SCLILLKTWLEIFPDNAGILRIYLLQFSAIVGYKTRRAA"
     gene            8905..10059
                     /gene="metK"
                     /locus_tag="ACHKBL_003694"
     CDS             8905..10059
                     /gene="metK"
                     /locus_tag="ACHKBL_003694"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HFO5820506.1"
                     /translation="MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACET
                     YVKTGMVLVGGEITTSAWVDIEEITRNTVREIGYVHSDMGFDANSCAVLSAIGKQSPD
                     INQGVDRADPLEQGAGDQGLMFGYATNETDVLMPAPITYAHRLVQRQAEVRKNGTLPW
                     LRPDAKSQVTFQYDDGKIVGIDAVVLSTQHSEEIDQKSLQEAVMEEIIKPILPAEWLT
                     SATKFFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFF
                     DLRPYGLIQMLDLLHPIYKETAAYGHFGREHFPWEKTDKAQLLRDAAGLK"
BASE COUNT         2558 a         2745 c         2595 g         2407 t
ORIGIN      
        1 tgttgtattt gtgatacata tcgccattta tttattgaat gtccgaaagc atacttttct
       61 aatgcaatgt tttttcgcta taccccttta tagattctga attctcacct gtcggctatt
      121 ttctcctcac ctcatgtgac atattcccca ctttactcaa ggaagattca tatgaaagca
      181 cctgtttcaa taacctcatt tcgcccacaa aaatctattg ctatcgtcat tggcgtgctt
      241 gccgtggttg tgctgccatt tctctcgtat tacaccgtta atgaaggtga acgcggcatt
      301 ttgctgcgct acggaaaaat cgtaaaagtt gccgagccgg gtctgggttt taagatccct
      361 tttatggaaa gcgtggagaa aatttccacg cgtaatcagg ctgtggttta tcagggatta
      421 caggcctaca gccgcgacca gcaaccggca caaatgacag tatcagtcag cttccacatt
      481 aaaccttcag aagcaggcgc ggtttatacc acctacaaca ccattgaagc attaaaagat
      541 cgtctgattg tccgtcagtt gccaactcaa ctggagaacg ttttcggcca atataccgcc
      601 atcagcgccg tacaggatcg tacaaaactg gttcaggatc tacaaaatgc aatgcgtaaa
      661 gccgttgttg gcccggtggt gattgatggt gtccagattg aaaatattga tttttctgac
      721 gcttatgaaa aatcgattga agatcgaatg aaagcagaag tcgccattgc cacacgtaag
      781 caaaatctgg aaacagagaa aatccaggcg caaatagcgg taactcaggc ccaggcagaa
      841 gcagacagta aactggcggc agccaaagca gaagctgaga ctattcgcgt aagaggcgcg
      901 gcagaggctg aaactattcg tttaaaaagt gcggctgaag cagaagcgat ccgcctgcgc
      961 ggtgaagctc tgcgggataa tcccggactt gttgccctca ccactgctga gcgttgggac
     1021 ggtaagctac ccgataccat gatccccggc agtacagttc cgtttatctc tactaagtaa
     1081 aaaaagggcc gctttcgcgg cccttctcac aaagcatctt accgattaca gcagcgcttt
     1141 cgctttcgca acaacgttgt caacggtgaa accgaactct tcaaacagtt gctctgcagg
     1201 agcagattca ccgaaggtgg tcatgccgac gatagcaccg ttcaggccaa catacttgta
     1261 ccagtagtca gcaatacccg cttccaccgc aacacgagcg gtaaccgctt tcggcagtac
     1321 ggattcacgg taagccgcat cctgcttgtc gaacgcgtcg gtagacggca tggaaaccac
     1381 gcgcgccttc acgccttcgg cagtcagttt ttcgtaggcg gcaacagcca gttcaacttc
     1441 cgaaccggtc gcgatgaaga tcagttccgg ctgaccggca cagtctttca gcacataacc
     1501 accgcgcgcg atgtttgcca gctgctcttc agtacgctcc tgctgcgcca ggttctgacg
     1561 ggagaggatc agcgcggtcg ggccgtcctg acgctcaacg ccgtatttcc acgcgaccgc
     1621 ggattcaacc tggtcacatg gacgccatgt ggacatgttc ggggtcacgc gcagagaagc
     1681 gacctgctct accggctggt gagtcgggcc atcttcgccc agaccgatgg agtcgtgggt
     1741 gtaaaccatc acctgacgct gtttcatcag cgcagccata cgtacggcgt tacgtgcgta
     1801 ttccacgaac atcaggaagg tggaggtgta cggcaggaaa ccaccgtgca aggagatacc
     1861 gttagcaatc gcggtcatac cgaactcgcg aacaccgtag tggatgtagt tacccgcagc
     1921 atcttcgttg attgctttag aaccagacca cagggtcagg ttagacggtg ccaggtcagc
     1981 agagccgccg aggaattccg gcaacagcgg gccgaaagct tcgatagcat tctgagacgc
     2041 tttacggctg gcgattttcg ccggattagc ctgcagttta gcgatgaatt ctttcgcttt
     2101 ggcatcgaag tcagacggca tttcgccttt catacggcgg gtaaattcag cggcttcctg
     2161 cggataagct ttggcgtaag ccgcgaattt ctcgttccat gcggattctt tcgcctggcc
     2221 tgcttctttc gcatcccact gagcatagat ttcagacggg atttcgaacg gtgcatattt
     2281 ccagcccagc tgttcgcggg tcagggcaat ttcagcgtcg cccagcggcg caccgtggga
     2341 gtcgtgcgta ccggctttgt tcggggaacc gaaaccgatg atggttttgc acatcagcag
     2401 ggacggtttg tcagtcactg cgcgcgcttc ttctactgcg cgtttgatgg atgccgcgtc
     2461 atgaccgtcg atgtcgcgaa taacgtgcca gccgtaagct tcgaaacgca ttgcggtgtc
     2521 gtcggtaaac cagccttcaa cgtgaccatc gatagaaata ccgttgtcat cgtagaacgc
     2581 aatcagttta cccagcttca gcgtacccgc cagagagcaa acttcgtggg agatgccttc
     2641 catcatgcag ccgtcgccca tgaaggcgta ggtgtagtgg tcgacgatgt cgtggcccgg
     2701 acggttaaac tgcgccgcca gcgttttttc tgcaatcgcc ataccgactg cgttggcaat
     2761 accctgaccc agcggacctg tagtggtttc cacgccagcg gtgtaaccca cttccggatg
     2821 gcccggagtt ttagagtgca gctgacggaa gtttttcagt tcttccatcg gcagatcgta
     2881 accggtgagg tgcagcaggc tgtagatcag catggagccg tggccgttgg acagcacgaa
     2941 gcggtcacgg tcagcccagg acggattctg cgggttgtgt ttcaggaaat cacgccacag
     3001 gacttcggca atgtcagcca tacccatcgg ggcgcccggg tgaccggatt tggctttctg
     3061 tactgcgtcc atgctcagcg cacgaatagc attggcaagc tctttacgtg aggacatttt
     3121 gactccagat cggatgatga agggcacgcc cttaacgact tgacgacagc gcgttttggg
     3181 ctacgccgga aaatttgcca acaatttacc gcaagccgcg cgtcatgtac atggaacatc
     3241 cttttgccgc ttcagaaatc tctggatcat gctcgcatgt tgcgcaatct gctcgcccgt
     3301 ccgctgcgct tttccttata ctgagactga gcgtcgattc acctgcaaac ggcgcatttt
     3361 tagaataatc ctgaccttgt gcggaagaga aaacatgaaa attcgcgcct tattggtagc
     3421 aatgagcgtg gcaacggtac tgaccggttg ccagaatatg gactccaacg gactgctctc
     3481 atcaggagcg gaagcttttc aggcttacag tttgagtgat gcgcaggtga aaaccctgag
     3541 cgatcaggca tgtcaggaga tggacagcaa ggcgacgatt gcgccagcca atagcgaata
     3601 cgctaaacgt ctgacaacta ttgccaatgc gctaggcaac aatatcaacg gtcagccggt
     3661 gaattacaaa gtgtatatgg cgaaggatgt gaacgccttt gcaatggcta acggctgtat
     3721 ccgcgtctat agcgggctga tggatatgat gacggataac gaagtcgaag cggtgatcgg
     3781 tcacgaaatg gggcacgtgg cgttaggcca tgtgaagaaa gggatgcagg tggcacttgg
     3841 tacaaatgcc gtgcgagtag ctgcggcctc tgcgggcggg attgtcggca gtttatctca
     3901 atcacaactc ggtgatctgg gcgagaaatt agttaattcg caattctccc agcgccagga
     3961 agcagaagcc gatgattatt cttacgatct tctgcgccaa cgcggcatca gcccggcagg
     4021 tcttgccacc agttttgaaa aactggcgaa gctggaagaa ggccgtcaaa gctcaatgtt
     4081 tgacgaccat ccagcatccg ccgaacgcgc ccagcatatt cgcgatcgta tgagcgcgga
     4141 tggagttaag taagcatttt attacgccgg atttgacgca taacgcatct tatccggcgc
     4201 ttactcgccc tttttcgctg cctgaatata cagcatctcc agcgccagcg tcgccgcagc
     4261 cagagcggtg atttccgact gatcgtatgc cggagccact tccactacgt ccatcccaac
     4321 aatgttgaga tctttcaggc cgcgtaccag tttaatagcg cgatcggagg tcaggccgcc
     4381 aattactggc gtaccggttc ccggcgcaaa tgcaggatcc aggcagtcga tatcaaaggt
     4441 caggtaaacc ggcatatcac ccacaatctg tttcacttgg gcgataacgt catccacgct
     4501 gcgatcgttc acctggcagg cgtccagcac ggtaaagccg ttgtctttat cgaactcagt
     4561 acgaatacca atctgcacgg aatgattcgg gtcgatcaga ccttctttcg gcgcggtata
     4621 gaacatggtg ccgtggtcaa attcacaacc gttcgcatag gtgtcggtgt gggcgtcaaa
     4681 gtgtaccagc gccattttgc cgaaatgctt cgcatgagca cgcagcagcg gcagcgtaac
     4741 aaagtggtca ccaccgaagg agagcatacg cttaccggca gccagcagct tctcggcgtg
     4801 cgcctgcagc ttctcgctca tctcacgggc atctccgaag gcatatacca gatcgccgca
     4861 gtccacgacg ttcaggcgct cacgcatgtc gaaattccac gggaagcgat tgtgttccca
     4921 ggccagattc gtcgaaacct gacggatcgc tgccggaccg tggcgaccac ccgcacgacc
     4981 agaagtggcc atatcgaacg gcacgccagt aatcacccag tcagcatcgc tgtcatatgg
     5041 ctggaagttc atcggcaggc gtaaaaaacc aaaggcgttg gaaaccagtg agttatcgta
     5101 ttgatgacct aaggtgctca tggatggact cctattacaa agtcgatata aaaaaaccct
     5161 tccgcgtcgt taggcccgac gaggaagggt tggatttgcg aaacacaatt ttgtgctaat
     5221 tatcgcctta aatgggcgag gcatcaagcg aggttgctat ttccgacacg gtggcggcgc
     5281 atagccattg ttatctgccg gaaaggcttg tccagagcac ggcggcagcg cgttttgcgc
     5341 cgggccttgt aattcaaagg cggcaaagcc gattacaccg gtattacgca catcgccttg
     5401 cgccgaattg gctgcgtagg aatcagcaac gttggcgaac tgaaaagccg cttcggtatg
     5461 tttatctttg cggaatcctt taatcgctaa cgaatctccg gcattcacaa tatagccgtt
     5521 gttattcagc gaaccttgct tgccgttcag cacatccagg ccatcaaccg tagcaacgat
     5581 ctcgtagttg gtattacgac tgtaattacg gatatataat tgataattca ttccgactct
     5641 ggcatggaca atatagcgtg gttcattatt ggatgaatca taaacccgcg taataggtag
     5701 agaattaaaa ttaccatcgc gaatagcata ttccagttcg ttaatacgaa tgctataaac
     5761 gcggtcatag ccggaaggaa cagtagtcga ataactaata acgatcatct ctgccggatc
     5821 gcgccagact cgttcagcag atacgccctg aaccgatgag tgtacatccc caccccaggc
     5881 ggtgccgaca ttgcctgatg cactgctatt atactgcgca ttactggaac aaccggccag
     5941 cagcacggct aatgctatca acgcgctacg tactttccat tttttcataa ccgttccctc
     6001 tgcatttaat taatgcagcg attgtagggg gattatttaa taagaaaagc agctaaatta
     6061 tttttaagaa tatataaaaa gaaagcgggc cgatttacgt aaataaatcg acccgccagc
     6121 ggaataatct taattattca tcttcaagat aggtataacc gtacaaacct gcctcgaact
     6181 cttcaaggaa ctgttgttgc agttcagcat caagatcggt tttcttcact tgatcacgga
     6241 actgggttaa caacgttttc ggatcgagct gtacatattg cagcatatcc gccacggtat
     6301 cgccttcgtc agacagttct acttctacgc tgccgtcagg gaagacgaac acgtcaaccg
     6361 cttcggtatc accgaacagg ttgtgcatgt tgccgaggat ctcctgatat gcgccgacca
     6421 tgaagaaacc gagcatcggc ggattctctg gatcgtactc cggcattggc attgtcgtgg
     6481 caataccatc accatcgatg tagtgatcaa tagcaccgtc agaatcacag gtaatatcca
     6541 gcagcacagc gcggcgctcc ggcacttgat ccaacccttc cagcggcaga accgggaaca
     6601 actggtcgat cccccatgcg tccggcatcg actggaacag cgagaagttg acgtacattt
     6661 tgtcagccat acgttcctgc agctcgtcga taatcggacg atgcgcacgg ttttgcggat
     6721 ccagttgctt ctgcacttca tggcacatgc tcagatagag ttgttctgcc catgcacgtt
     6781 cttgcaggct aaaggtgccg gaagagtagc cgatatgaat atcgtgcaga tccatctggc
     6841 tgtcgtgtaa ccattcacgc agagaacgac gagttcccgg ttcgtgcatc tcctgccagg
     6901 tttcccacat gctttgcagg gcgcgcggtg cgtcttctgc aggcgcggtc ggcaccgtgt
     6961 attcgttacg ttccacgccg atgatattcg acaccagcac ggtgtgatgc gcagtcaccg
     7021 cacgacccga ctcggtgatt accgtcggat gtggcagacc attttcttca cacgcatcac
     7081 caatcgccca gatgatgttg ttggcgtatt cattgaggcc gtagttcacc gaacagtcgg
     7141 actgcgaacg ggtgccttca tagtccacgc ccagaccgcc gccaacgtcg aagcactgaa
     7201 tattgacgcc cagcttgtgc agttccacat agaaacgcgc ggattcacga acgcctgtcg
     7261 cgatatcgcg aatattcgcc atctgcgaac cgaggtggaa gtgcagcagt tgcaggctgt
     7321 cgagacgccc ggcttcacgc agcgtttcaa ccagttgcag tacctgagtc gccgccaggc
     7381 cgaacttcga tttttccccg ccagaggact gccatttacc cgagccctgc gaagccagac
     7441 gtgcacgcac gcccagacga ggaacgacat tcagacgttc tgcttcatcc agcacaatgg
     7501 caatttctga catcttctca atgaccagat agaccttgtg gcccatcttc tcgccaatta
     7561 atgccaggcg gatatattcg cggtccttat aaccgttgca gacgatgacg ctacgggtca
     7621 tgccagcatg tgccagcacc gccatcaact ccgctttgga accggcttcc aggcccagcg
     7681 gttcgcccga atgaatcagg gactcaatca cgcggcggtg ctggttaact ttgatcggat
     7741 aaacgaggaa gtaatcgccg ttatagccgt aggattcccg cgcacgtttg aacgcggcgt
     7801 taatagaacg caaacggtgc tgcaggatct gtgggaaaca gaacagtgca ggcagacgct
     7861 ggccctgtgc ttcacgagtt ttcactaact gcgcgagatc gacgcgagct tccgggacgt
     7921 ccgggtccgg gcacacgcta atgtggccca gctcgttaac gtcatagtag ttattgcccc
     7981 accaggcaat attgtaagta cgcagcatct tgctggcttc ctgggagctc attgcaacct
     8041 cctgcatgga gcgtagtaca ccgtgttcgc ccgctgacga aggcaaaccc atagacatgt
     8101 cgtcagacat agcgaacctc aaattatttt attaagtgta aaacagttaa cgactatcgc
     8161 agcctggaac tgcgataaca acccataagc acatggattt tccagcagtg aatgctgacg
     8221 ctccaactgc gcaaccggtt tctttttcat aacattatta agcacataac cgaacgtaag
     8281 tgtgacagtt cggcgaaacc acgagaaaac tcttgttttt acaagagcgc ccttgttcag
     8341 tcctcagtaa ctgtaaccag ctcttgaatc ctgagaagcg ccgagatggg tataacatcg
     8401 gcaggtatgc aaagcagaga tgcagagtgc ggggaacgaa tcttcaccag aacggtgaga
     8461 caggttaagc agcagacaac ggttcattat ttcgtatcac ctccacggcc gcctgttaag
     8521 acgaacccac aagccaaaac tctgatttca acccggctgg aagtggcaac acgaaagaaa
     8581 cgtcgtgtgc tttttattta agccgcgcgc cgcgttttat accccacaat ggcagaaaat
     8641 tgcaaaagat aaatacgcag aatgccagca ttgtcaggaa aaatttccag ccacgttttt
     8701 aacagaatga gacacgattc aaaaaaaagt ggaaatcggg tgaagaattg acctaaaata
     8761 gccatccaga tgttaatcca tccataccga ttaacactca gactgccagt gtttttaacc
     8821 tgcagagtcg tggtaggatc cgctaccaca gaaaatccac acaacagttt gagctaacca
     8881 aattctcttt aggtgatatt aaatatggca aaacaccttt ttacgtccga gtccgtctct
     8941 gaagggcatc ctgacaaaat tgctgaccaa atttctgatg ccgttttaga cgcgatcctc
     9001 gaacaggatc cgaaagcacg cgttgcttgc gaaacctacg taaaaaccgg catggtttta
     9061 gttggcggcg aaatcaccac cagcgcctgg gtagacatcg aagagatcac ccgtaacacc
     9121 gttcgcgaaa ttggctatgt gcattccgac atgggctttg acgctaactc ctgtgcggtt
     9181 ctgagcgcta tcggcaaaca gtctcctgac atcaaccagg gcgttgaccg tgccgatccg
     9241 ctggaacagg gcgcgggtga ccagggtctg atgtttggct acgcaaccaa tgaaaccgac
     9301 gtgctgatgc cagcgcctat cacctatgca caccgtctgg tacagcgtca ggctgaagtg
     9361 cgtaaaaacg gcactctgcc gtggctgcgc ccggacgcga aaagccaggt gactttccag
     9421 tatgacgacg gcaaaatcgt tggtatcgat gctgtcgtgc tttccactca gcactctgaa
     9481 gaaatcgacc agaaatcgct gcaagaagcg gtaatggaag agatcatcaa gccgattctg
     9541 ccggctgaat ggctgacttc tgccaccaaa ttcttcatca acccgaccgg tcgttttgtt
     9601 atcggtggcc cgatgggtga ctgcggtctg actggtcgta aaattatcgt tgatacctac
     9661 ggcggcatgg cacgtcacgg tggcggtgca ttctccggta aagatccatc aaaagtggac
     9721 cgttccgcag cgtacgcagc acgttatgtc gcgaaaaaca tcgtggctgc tggcctggcc
     9781 gatcgttgtg aaattcaggt ttcctacgca atcggcgtgg ctgaaccgac ttccatcatg
     9841 gtagaaactt tcggtactga gaaagtgcct tctgaacaac tgactctgct ggtacgtgaa
     9901 ttcttcgacc tgcgcccata cggtctgatt cagatgctgg atctgctgca cccgatctac
     9961 aaagaaaccg cagcatacgg tcactttggt cgtgaacatt tcccgtggga aaaaaccgac
    10021 aaagcgcagc tgctgcgtga tgctgccggt ctgaagtaat ctttcttcac ctgctttcaa
    10081 aggccagcct cgcgctggcc tttttctttt acctcaccgc ggtggacagg cattcacgcc
    10141 gcatccggca aaaaaaccgc ccgcacaata acatcattcc tcctcaacac gttttaccgc
    10201 aaattaccat cactactgaa accgattaca ccaaccacgc cagccaaata tttgtaatat
    10261 tttcatatta ttattcagct ttcacagttg ttacatttct tttca
//
LOCUS       DAZFLD010000167        10246 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.168, whole genome shotgun sequence.
ACCESSION   DAZFLD010000167 DAZFLD010000000
VERSION     DAZFLD010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10246
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.168"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            61..1455
                     /gene="galP"
                     /locus_tag="ACHKBL_003695"
     CDS             61..1455
                     /gene="galP"
                     /locus_tag="ACHKBL_003695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/proton symporter"
                     /protein_id="HFO5820507.1"
                     /translation="MPDAKKQGRSNKAMTFFVCFLAALAGLLFGLDIGVIAGALPFIA
                     DEFQITSHTQEWVVSSMMFGAAVGAVGSGWLSFKLGRKKSLMIGAILFVAGSLFSAAA
                     PNVEVLILSRVLLGLAVGVASYTAPLYLSEIAPEKIRGSMISMYQLMITIGILGAYLS
                     DTAFSYTGAWRWMLGVIIIPAILLLIGVFFLPDSPRWFAAKRRFVDAERVLLRLRDTS
                     AEAKRELDEIRESLQVKQSGWALFKENSNFRRAVFLGVLLQVMQQFTGMNVIMYYAPK
                     IFELAGYTNTTEQMWGTVIVGLTNVLATFIAIGLVDRWGRKPTLTLGFLVMAAGMGVL
                     GTMMHIGIHSPSAQYFAIAMLLMFIVGFAMSAGPLIWVLCSEIQPLKGRDFGITCSTA
                     TNWIANMIVGATFLTMLNTLGNANTFWVYAALNVLFILLTLWLVPETKHVSLEHIERN
                     LMKGRKLREIGAHD"
     gene            1532..2029
                     /locus_tag="ACHKBL_003696"
     CDS             1532..2029
                     /locus_tag="ACHKBL_003696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HFO5820508.1"
                     /translation="MKTSRLPIAIQQAVMRRLREKLAQANLKLGRNYPEPKLSYTQRG
                     TSAGTAWLESYEIRLNPVLLLENSEAFIEEVVPHELAHLLVWKHFGRVAPHGKEWKWM
                     MESVLGVPARRTHQFELQSVRRNTFPYRCKCQEHQLTVRRHNRVVRGEAVYRCVHCGE
                     QLVAK"
     gene            2124..2831
                     /gene="endA"
                     /locus_tag="ACHKBL_003697"
     CDS             2124..2831
                     /gene="endA"
                     /locus_tag="ACHKBL_003697"
                     /EC_number="3.1.21.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease I"
                     /protein_id="HFO5820509.1"
                     /translation="MYRYLSIAAVVLSAAFSGPALAEGINSFSQAKAAAVKVHADAPG
                     TFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRVEWEHVVPAWQFGHQRQCWQDGG
                     RKNCAKDPVYRKMESDMHNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
                     AAEPPARARGAIARTYFYMRDQYNLTLSRQQTQLFNAWDKMYPVTDWECERDERIAKV
                     QGNHNPYVQRACQARKS"
     gene            2911..3642
                     /gene="rsmE"
                     /locus_tag="ACHKBL_003698"
     CDS             2911..3642
                     /gene="rsmE"
                     /locus_tag="ACHKBL_003698"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311849.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HFO5820510.1"
                     /translation="MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFD
                     GSNQVFDAEITSASKKSVEVKVLEGQIDDRESPLHIHLGQVMSRGEKMEFTIQKSIEL
                     GVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWC
                     AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLG
                     PRVLRTETTALTAITALQVRFGDLG"
     gene            3655..4605
                     /gene="gshB"
                     /locus_tag="ACHKBL_003699"
     CDS             3655..4605
                     /gene="gshB"
                     /locus_tag="ACHKBL_003699"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HFO5820511.1"
                     /translation="MIKLGIVMDPIANINIKKDSSFAMLLEAQRRGYELHYMEMGDLY
                     LINGEARAHTRTLNVKQNYEEWFSFVGEQDLPLADLDVILMRKDPPFDTEFIYATYIL
                     ERAEEKGTLIVNKPQSLRDCNEKLFTAWFSDLTPETLVTRNKAQLKAFWEKHSDIILK
                     PLDGMGGASIFRVKEGDPNLGVIAETLTEHGTRYCMAQNYLPAIKDGDKRVLVVDGEP
                     VPYCLARIPQGGETRGNLAAGGRGEPRPLTESDWKIARQIGPTLKEKGLIFVGLDIIG
                     DRLTEINVTSPTCIREIEAEFPVSITGMLMDAIEARLQQQ"
     gene            4714..5277
                     /locus_tag="ACHKBL_003700"
     CDS             4714..5277
                     /locus_tag="ACHKBL_003700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417423.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HFO5820512.1"
                     /translation="MNLQHHFLIAMPALQDPIFRRSVVYICEHNTNGAMGIIVNKPLE
                     NLKIEGILEKLKITPEPRDESIRLDKPVMLGGPLAEDRGFILHTPPSNFASSIRISDN
                     TVMTTSRDVLETLGTDKQPSDVLVALGYASWEKGQLEQEILDNAWLTAPADLNILFKT
                     PIADRWREAAKLIGVDILTMPGVAGHA"
     gene            5277..5693
                     /gene="ruvX"
                     /locus_tag="ACHKBL_003701"
     CDS             5277..5693
                     /gene="ruvX"
                     /locus_tag="ACHKBL_003701"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HFO5820513.1"
                     /translation="MSGTLLAFDFGTKSIGVAVGQRITGTARPLPAIKAQDGTPDWNI
                     IERLLKEWQPDEIIVGLPLNMDGTEQPLTARARKFANRIHGRFGVEVKLHDERLSTVE
                     ARSGLFEQGGYRALNKGKVDSASAVIILESYFEQGY"
     gene            complement(5867..6847)
                     /locus_tag="ACHKBL_003702"
     CDS             complement(5867..6847)
                     /locus_tag="ACHKBL_003702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HFO5820514.1"
                     /translation="MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDAPDV
                     EELLREWLDDDQRTILLENGQLDFAVSLAENQRLRGSAFAQRQGISLALRLLPSHCPQ
                     LEQLGAPPVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
                     YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETG
                     HLVLATLHTRGAAQAVERLVDSFPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVAL
                     FELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMITFQQSYQQRVKEGRL"
     gene            6865..7569
                     /gene="yggS"
                     /locus_tag="ACHKBL_003703"
     CDS             6865..7569
                     /gene="yggS"
                     /locus_tag="ACHKBL_003703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate homeostasis protein"
                     /protein_id="HFO5820515.1"
                     /translation="MNDIAHNLAQVRDKISAAATRCGRSPEEITLLAVSKTKPASAIA
                     EAIDAGQRQFGENYVQEGVDKIRHFQELGVTGLEWHFIGPLQSNKSRLVAEHFDWCHT
                     IDRLRIATRLNDQRPAELPPLDVLIQINISDENSKSGIQLAELDELAAAVAELPRLRL
                     RGLMAIPAPESEYVRQFEVARQMAVAFAGLKTRYPHIDTLSLGMSDDMEAAIAAGSTM
                     VRIGTAIFGARDYSKK"
     gene            7587..8153
                     /gene="yggT"
                     /locus_tag="ACHKBL_003704"
     CDS             7587..8153
                     /gene="yggT"
                     /locus_tag="ACHKBL_003704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic shock tolerance protein YggT"
                     /protein_id="HFO5820516.1"
                     /translation="MNTLTFLLSTVIELYTMVLLLRIWMQWAHCDFYNPFSQFVVKVT
                     QPIIGPLRRVIPAMGPIDSASLLVAYILSFIKAIVLFKVVTFLPIIWIAGLLILLKTI
                     GLLIFWVLLVMAIMSWVSQGRSPIEYVLIQLADPLLRPIRRLLPAMGGIDFSPMILVL
                     LLYVINMGVAEVLQATGNMLLPGLWMAL"
     gene            8150..8440
                     /gene="yggU"
                     /locus_tag="ACHKBL_003705"
     CDS             8150..8440
                     /gene="yggU"
                     /locus_tag="ACHKBL_003705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 family protein YggU"
                     /protein_id="HFO5820517.1"
                     /translation="MSAVTVNDDGLVLRLYIQPKASRDSIVGLHGDEVKVAITAPPVD
                     GQANSHLVKFLGKQFRVAKSQVVIEKGELGRHKQIKIINPQQIPPEIAALIN"
     gene            8448..9041
                     /locus_tag="ACHKBL_003706"
     CDS             8448..9041
                     /locus_tag="ACHKBL_003706"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HFO5820518.1"
                     /translation="MQKVVLATGNAGKVRELASLLSDFGLDIVAQTDLGVDSAEETGL
                     TFIENAILKARHAAKVTGLPAIADDSGLAVDALGGAPGIYSARYSGEDATDQKNLQKL
                     LETMKDVPDDQRQARFHCVLIYLRHAEDPTPLVCHGSWPGVITREPAGTGGFGYDPIF
                     FVPSEGKTAAELTREEKSAISHRGQALKLLLDALRNG"
     gene            9034..10170
                     /gene="hemW"
                     /locus_tag="ACHKBL_003707"
     CDS             9034..10170
                     /gene="hemW"
                     /locus_tag="ACHKBL_003707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HFO5820519.1"
                     /translation="MVKLPPLSLYIHIPWCVQKCPYCDFNSHALKGEVPHDDYVQHLL
                     NDLDNDVAYAQGREVQTIFIGGGTPSLLSGPAMQTLLDGVRARLPLAADAEITMEANP
                     GTVEADRFVDYQRAGVNRISIGVQSFSEEKLKRLGRIHGPQEAKRAAKLASGLGLRSF
                     NLDLMHGLPDQSLEEALGDLRQAIELNPPHLSWYQLTIEPNTLFGSRPPVLPDDDALW
                     DIFEQGHQLLTAAGYQQYETSAYAKPGYQCQHNLNYWRFGDYIGIGCGAHGKVTFPDG
                     RILRTTKTRHPRGFMQGRYLESQRDVEAADKPFEFFMNRFRLLEAAPRVEFSQYTGLS
                     EEVIRSQLDEAIAQGYLTECADYWQITEHGKLFLNSLLELFLAE"
BASE COUNT         2398 a         2704 c         2706 g         2438 t
ORIGIN      
        1 ttttacgtcg tactcaccta tcttaattca caataaaaaa taaccatatt ggagggcatc
       61 atgcctgacg ctaaaaaaca ggggcggtca aacaaggcaa tgacgttttt cgtctgcttc
      121 cttgccgctc tggcgggatt actctttggc ctggatatcg gtgtaattgc tggcgcactg
      181 ccgtttattg cagatgaatt ccagattact tcgcacacgc aagaatgggt cgtaagctcc
      241 atgatgttcg gtgcggcagt cggtgcggtg ggcagtggct ggctctcctt taaactcggg
      301 cgcaaaaaga gcctgatgat cggcgcaatt ctgtttgttg ccggttcact gttctctgcg
      361 gctgcgccaa acgttgaagt actgattctt tcccgcgttc tgctggggtt ggcggtgggt
      421 gtggcttctt ataccgcacc gctgtacctc tctgaaattg cgccggaaaa aattcgcggc
      481 agcatgatct cgatgtatca gttgatgatc actatcggga tcctcggtgc ttatctttct
      541 gataccgcct tcagctacac cggtgcatgg cgctggatgc tgggtgtgat tatcatcccg
      601 gcaattttgc tgctgattgg tgtcttcttc ctgccagaca gtcctcgttg gtttgccgcc
      661 aaacgccgtt ttgttgatgc cgaacgcgtg ctgctacgcc tgcgtgacac cagcgcggaa
      721 gcgaaacgcg aactggatga aatccgtgaa agtttgcagg ttaaacagag tggctgggcg
      781 ctgtttaaag agaacagcaa cttccgccgc gcggtgttcc ttggcgtact gttgcaggta
      841 atgcagcaat tcaccgggat gaacgtcatc atgtattacg cgccgaaaat cttcgaactg
      901 gcgggttata ccaacaccac cgagcaaatg tgggggacgg tgatcgtcgg cctgaccaac
      961 gtactcgcca cctttatcgc aatcggcctt gttgaccgct ggggacgtaa accaacgcta
     1021 acgctgggct tcctggtgat ggctgctggc atgggcgtac tcggtacaat gatgcatatc
     1081 ggtatccact ctccgtcggc gcagtatttc gccatcgcca tgctgctgat gtttattgtc
     1141 ggttttgcca tgagtgccgg tccgctgatt tgggtactgt gctccgaaat tcagccgctg
     1201 aaaggccgcg attttggcat cacctgctcc accgccacca actggattgc caacatgatc
     1261 gttggcgcaa cgttcctgac catgctcaac acgctgggta acgccaacac cttctgggtg
     1321 tatgcggctc tgaacgtact gtttatcttg ctgacattgt ggctggtacc ggaaaccaaa
     1381 cacgtttcgc tggaacatat tgaacgtaat ctgatgaaag gtcgtaaact gcgcgaaatc
     1441 ggcgctcacg attaatctcc tcaggcttcc tcccgtcgcg gaggaagcca cctcttgcag
     1501 tcatcttttc ttcgctctat cctctgccgc tatgaaaaca tcccgtctcc ctatcgccat
     1561 ccagcaggcc gttatgcgcc gcctgcggga aaaactcgcc caggccaacc tgaagctagg
     1621 gcgtaactac ccggagccaa aactctctta cacccagcgc ggaacctccg ccggaacagc
     1681 ctggctggaa agctatgaaa tccgcctcaa tcccgttttg ctgttggaaa acagtgaagc
     1741 ttttattgaa gaagtggtac cgcacgaact ggcacatttg ctggtatgga aacatttcgg
     1801 ccgcgtagcg ccacatggca aagagtggaa gtggatgatg gaaagcgtgc tgggcgttcc
     1861 cgcccgtcgt acgcatcagt tcgaactgca atccgtgcgt cgcaacacct tcccctaccg
     1921 ctgcaagtgc caggagcatc agcttaccgt acgccgccat aatcgcgtag ttcgtggcga
     1981 ggccgtctat cgctgtgttc actgcggtga acagctggtt gcgaaataac cacctgaact
     2041 atcaggaact ttcctgatct ggctgattgc ataccaaaac aactttcgct acgttgctgg
     2101 ctcgttttaa cacggagtaa gtgatgtacc gttatttgtc tattgctgcg gtggtactga
     2161 gcgcagcatt ttccggcccg gcgttggccg aaggtatcaa tagtttttct caggcgaaag
     2221 ccgcggcggt aaaagtccac gctgatgcgc ccggtacgtt ttattgcgga tgtaaaatta
     2281 actggcaggg caaaaaaggc gttgttgatc tgcaatcgtg cggctatcag gtgcgcaaaa
     2341 atgaaaaccg cgccagccgc gtagagtggg aacacgtcgt tcccgcctgg caattcggtc
     2401 accagcgcca gtgctggcag gacggtggac gtaaaaactg cgccaaagat ccggtctatc
     2461 gcaagatgga aagcgatatg cataacctgc agccgtcagt cggtgaggtg aatggcgatc
     2521 gcggcaactt tatgtacagc cagtggaatg gcggcgaagg ccagtacggt caatgcgcca
     2581 tgaaggtcga tttcaaagaa aaagccgccg aaccaccagc gcgtgcacgc ggtgccattg
     2641 cgcgcaccta cttctatatg cgtgaccaat acaacctgac actctctcgc cagcaaacgc
     2701 aactgttcaa cgcatgggac aagatgtatc cggttaccga ctgggagtgc gagcgcgatg
     2761 aacgcatcgc gaaggtgcag ggcaatcata acccgtatgt gcaacgcgct tgccaggcgc
     2821 gaaagagcta acctacacta gcgggattct ttttgttaac ccctacccca cgcgtacaac
     2881 cgcgtgggga gacgacgcgg atttttaact atgcgtatcc cccgcattta tcatcctgaa
     2941 ccactgacca gccattctca catcgcgctt tgcgaagatg ccgccaacca tatcgggcgc
     3001 gtactgcgta tggggccggg gcaggcgttg caattgtttg acggtagcaa ccaggtcttt
     3061 gacgccgaaa ttaccagcgc cagcaaaaaa agcgtggaag tgaaggtact ggaaggccag
     3121 atcgacgatc gcgaatctcc gctgcatatt cacctcggtc aggtgatgtc gcgtggtgaa
     3181 aaaatggaat ttacgatcca gaaatcgatc gaactcggtg taagcctcat tacgccactt
     3241 ttttctgagc gctgcggcgt taaactggat agtgaacgtc tgaacaagaa gctccagcag
     3301 tggcagaaga ttgcaattgc tgcctgtgag cagtgtggtc gtaaccgggt gccggaaatc
     3361 cgtccagcga tggatctgga agcctggtgt gcagagcagg atgaaggact gaaactgaat
     3421 cttcacccgc gcgccagtaa cagcatcaat acgttgccgt taccggttga acgtgtccgc
     3481 ctgctgattg gcccggaagg cggtttatcg gcagatgaaa ttgccatgac tgcccgctat
     3541 caatttactg atatcctgtt gggacctcgc gttttgcgta cagagacaac tgcgctcacc
     3601 gccattaccg cgctacaagt acgatttggc gatttgggct aacggagaag aataatgatc
     3661 aagctcggca tcgtgatgga ccccatcgca aacatcaaca tcaagaaaga ttccagtttc
     3721 gctatgttgc tggaagcaca gcgtcgtggt tacgaacttc actatatgga gatgggcgat
     3781 ctgtatctga tcaatggcga agcccgcgcc catacccgca cgctgaacgt gaagcagaac
     3841 tacgaagagt ggttttcatt cgtcggtgaa caggatctgc cgctggccga tctcgatgtg
     3901 atcctgatgc gtaaagaccc gccgtttgat accgagttta tctacgcgac ctatattctg
     3961 gaacgtgccg aagagaaagg gacgctgatc gttaacaagc cgcagagcct gcgcgactgt
     4021 aacgagaaac tgtttaccgc ctggttctct gacttaacgc cagaaacgct ggttacgcgc
     4081 aataaagcgc agctaaaagc gttctgggag aaacacagcg acatcattct taagccgctg
     4141 gacggtatgg gcggcgcgtc gattttccgc gtgaaagaag gcgatccaaa cctcggcgtg
     4201 attgccgaaa ccctgactga gcatggcact cgctactgca tggcgcaaaa ttacctgcca
     4261 gccattaaag atggcgacaa acgcgtgctg gtggtggatg gcgagccggt accgtactgc
     4321 ctggcgcgta ttccgcaggg gggcgaaacc cgtggcaatc tggctgccgg tggtcgcggt
     4381 gaacctcgtc cgctgacgga aagtgactgg aaaatcgccc gtcagatcgg gccgacgctg
     4441 aaagaaaaag ggctgatttt tgttggtctg gatatcattg gcgaccgtct gactgaaatt
     4501 aacgtcacca gcccaacctg tattcgtgag attgaagcag agtttccggt gtcgatcacc
     4561 ggaatgttaa tggatgccat cgaagcacgt ttacagcagc agtaacccac cttagcgaga
     4621 aggatctcgt tgagactctg agtgacagcg cccttctttc cacgcatact gggcgctgtt
     4681 gcttttttga accaggaaac agaacctctg acaatgaatt tacagcatca ctttcttatt
     4741 gccatgcctg ctctccagga tccgattttc cgtcgttccg tggtctacat ttgcgaacat
     4801 aataccaatg gtgcaatggg gatcatcgtc aacaagccgc tggaaaatct caaaattgaa
     4861 gggattctgg aaaagctgaa gatcacgccg gagccgcgtg atgaatcaat ccgtctggat
     4921 aaaccggtta tgctcggtgg tccgctggct gaagatcgcg ggtttatttt gcatactccg
     4981 ccctctaatt ttgcttccag cattcgcatt tcagacaaca cggtgatgac cacttcccgc
     5041 gatgtgctgg aaacgctcgg caccgataaa caaccgtctg acgtattggt ggctctgggc
     5101 tatgcctcct gggagaaagg tcaactggaa caagaaattc tcgataacgc gtggctaacg
     5161 gccccggcgg atctgaatat tctgttcaaa acaccgattg ccgaccgctg gcgcgaggcg
     5221 gcaaaactga ttggtgtgga tattctcacc atgcctggtg tggcagggca cgcctgatga
     5281 gtggaacctt actcgccttc gacttcggta ccaaaagcat tggtgtagcg gtcggccaac
     5341 gcattaccgg caccgctcgc ccattgcctg caattaaagc acaggacggt acgccggact
     5401 ggaacattat cgagcgttta ctgaaagagt ggcagcctga cgaaatcatc gtcggtttgc
     5461 cgctgaatat ggacggcacc gaacagccgt taaccgccag agcgcgtaaa tttgccaacc
     5521 gtattcatgg tcgtttcggt gttgaagtaa agctccatga cgagcgtctt agcaccgtgg
     5581 aggcacgttc cggtctgttt gaacagggcg gctatcgggc gctcaacaaa ggcaaagtcg
     5641 actctgcctc tgcggttatt attctcgaaa gctatttcga gcagggatat taaggcgatt
     5701 tactagcgca cagatttctg acaatgtgct cgttgtttca tgttggatgc ggcgtaaacg
     5761 ccttatccaa cctacagtga gtgcgaattc aaagttacgc ttcccttgta ggcccgataa
     5821 gcttgcgcat cgggcatatt tacaattcgt atcagacaca gcaacgtcac aagcgccctt
     5881 ctttcacccg ctgctgataa ctctgctgaa acgttatcat ccccacctgc tgcccggttt
     5941 gaataacatg cggtaactgg tgggttttcc cttcacgaat caaattcccc accgcgggtg
     6001 tgttaatcag caattcaaac aacgccacgc gtccttcctg tttatccact tccagctttt
     6061 gtgacaacac cgcccgtaaa ctacctgcca gttgattacg cacgggatct ttttcctgcg
     6121 ccggaaatga atctaccagt cgctcaactg cctgcgccgc accacgcgta tgtaatgttg
     6181 ccagcaccaa atgcccggtt tctgccgccg tcagcgccag acggattgtc tcgctgtcac
     6241 gcagctctcc gagcaaaatc acatcaggat cttcccgcaa tgcagcccgc aatcccgatg
     6301 cgaacgtcat acaatgcaaa ccgatttccc gctgctggat caaacatcgc tggcttgcat
     6361 agagatattc gacaggatct tccagcgtca gaatatgcgc atcggcatgt tgattgagat
     6421 agccaaccat cgccgccagc gtggtagatt tgccgctccc cgtcgccccc gtcaccagaa
     6481 tcaggccatt ctcgctcttg agtaattccg gcaataccgg tggcgcacca agctgttcga
     6541 gctgtggaca gtgcgaaggt aacaaccgta atgccagaga aataccttgc cgttgcgcga
     6601 acgcactgcc acgcaaccgc tggttttccg ccagcgacac agcaaaatcc aactgaccat
     6661 tctccagcaa tattgtccgc tgatcgtcat ccagccactc ccgcagtagc tcttcgacgt
     6721 ccggcgcatc aaacggcgca gcttccattc gcccgcgaat gcgccatcgt gcgggccagg
     6781 cgctgcacag gtgtagatcc gagacgttat gctttacact aagggccaca atttcttcca
     6841 tattcatact aagatcctcg gaaaatgaac gatattgcgc ataacctggc acaggtccgg
     6901 gacaaaatct cagcggctgc aacgcgttgc ggccgttctc cagaagaaat tacgctgctt
     6961 gcagtcagta aaacaaaacc tgcgagcgcc atcgcagaag ccattgatgc cgggcagcgt
     7021 caatttggtg aaaactacgt tcaggaaggg gtagataaaa ttcgccactt tcaggaactg
     7081 ggcgtaacag gattagaatg gcattttatt ggcccgttgc agtctaataa aagccgcctg
     7141 gtggcagagc atttcgactg gtgtcatacc atcgaccgtt tgcgcattgc tacccgcctc
     7201 aacgatcagc gcccggcaga acttccccct cttgacgtac tgattcaaat taatattagt
     7261 gatgaaaaca gtaagtccgg gattcaactg gcagaactgg acgagctggc agctgcggtc
     7321 gctgaactac cgcgtttacg tctgcgcggg ctgatggcaa tccctgcgcc tgagtcagaa
     7381 tatgtaaggc agtttgaagt tgcacgccaa atggctgtag catttgccgg actgaaaacg
     7441 cgctacccgc atatcgacac gctctctctg ggaatgtcgg acgatatgga agccgccatt
     7501 gcggcaggta gcacgatggt tcgtatcggc actgcaattt ttggtgcgcg tgattactct
     7561 aaaaaataag gaattaaagg aacgccatga atacgttgac tttcctgctt tcaacggtca
     7621 ttgagctgta taccatggtg ctgttattac gcatctggat gcagtgggct cattgtgatt
     7681 tttacaaccc cttctcacag tttgtagtga aggtaacgca gccaattatc gggccactgc
     7741 gccgcgttat tccggcaatg ggacccattg acagcgcctc gctgctggtt gcctatattc
     7801 tcagttttat caaagccatc gtgctgttta aagtggtgac cttcctgcca atcatctgga
     7861 ttgccggttt actgattctg ctgaaaacca tcggcctgct gattttctgg gtcctgctgg
     7921 tgatggcgat tatgagctgg gtaagccagg gtcgtagtcc gattgaatac gtgctgattc
     7981 agcttgccga tccgctgctg cgcccgattc gccgcctgct accggcaatg ggtgggattg
     8041 atttctcgcc gatgatcctc gttctgctgc tgtatgtcat caatatgggt gtcgcagaag
     8101 tattacaggc aaccggaaac atgctgctgc cggggctgtg gatggcgtta tgagtgccgt
     8161 aacagttaat gatgacggtc tggttttacg gctctatatt cagccgaaag ccagccgtga
     8221 ttctattgtc ggtttacatg gcgacgaggt aaaagtcgcc attaccgcgc cgccggttga
     8281 cggccaggcc aacagccatc tggtgaagtt tctcggtaag caattccggg ttgccaaaag
     8341 ccaggtggtg attgaaaaag gcgaacttgg ccgccacaaa caaattaaaa tcattaatcc
     8401 gcaacaaatc ccgccagaaa tcgcggcgtt aattaattag gtatcctatg caaaaagttg
     8461 tcctcgcaac cggcaatgcc ggtaaagtgc gtgagctggc gtcgctgctt agcgacttcg
     8521 gtcttgatat cgtggcccaa acagacctcg gcgtagattc cgctgaagaa accggcctga
     8581 cctttatcga aaacgcgatt ctgaaagcgc gccatgcggc aaaagtgacc ggtttaccgg
     8641 caattgccga cgactccggt ctggcggtag atgcgctggg tggcgcgccg ggtatttact
     8701 ccgcgcgtta ttccggtgaa gacgcgaccg atcaaaagaa cctgcaaaaa ctgctggaaa
     8761 caatgaaaga cgtaccggac gaccaacgtc aggcgcgttt ccactgcgtg ctgatatatc
     8821 tgcgtcacgc ggaagatccg actccgctgg tgtgccacgg tagctggccg ggcgtgatta
     8881 ctcgtgaacc agcgggtact ggtggctttg gttatgatcc aattttcttc gttccttctg
     8941 aagggaaaac cgctgccgaa ctgacccgcg aagaaaagag cgccatttcc caccgtggtc
     9001 aggcattgaa actgctgctg gacgctttac gtaatggtta aattaccgcc gctgagtctc
     9061 tacattcaca tcccgtggtg cgtgcagaaa tgcccgtact gcgatttcaa ctctcacgcg
     9121 ttgaaaggag aagtgccgca cgacgattat gttcagcatc tgcttaacga tctggacaac
     9181 gatgtggctt acgctcaggg ccgtgaagtg cagacaatct ttattggcgg tggtacgccg
     9241 agcctgcttt ccggcccggc gatgcaaacg ctgctggacg gcgtgcgtgc gcgtttgccg
     9301 ctggcagcgg atgcagaaat tacgatggaa gcgaacccag gcacggtaga agcggatcgc
     9361 tttgtcgatt atcagcgtgc tggtgtgaac cgcatctcta ttggtgtgca gagttttagc
     9421 gaagaaaagc tgaaacgact cgggcgtatt catggcccgc aagaagcgaa acgagcggca
     9481 aagctggcga gcggtttagg gttacgtagt tttaaccttg atttgatgca tgggctgccg
     9541 gatcaatcac tggaagaggc gctaggcgat ctgcgccagg ctattgaact gaatccgcca
     9601 catctttcct ggtatcaact gaccatcgaa cctaatacgc tgtttggctc gcgccctcct
     9661 gtactgccgg acgatgacgc gctgtgggat attttcgaac aggggcatca gttattaacc
     9721 gcagcgggct atcagcagta tgaaacatcc gcttacgcca aacccggtta tcagtgccag
     9781 cacaatctca actactggcg atttggcgac tacatcggta ttggctgcgg cgcgcacggc
     9841 aaagtgacct tcccggatgg gcgcattctg cgtaccacca aaacgcgtca tccgcgtggt
     9901 tttatgcagg gaagatatct ggaaagccag cgtgatgtcg aagctgcaga taaaccgttt
     9961 gagttcttta tgaatcgctt ccgtctgctt gaggccgcgc cgcgcgtgga gtttagtcag
    10021 tatactggcc tttctgaaga agttattcgc tctcagttag acgaggctat cgctcagggt
    10081 tatctcacag aatgtgcgga ttactggcag ataaccgagc atggtaaatt attcttaaac
    10141 tcacttcttg aattattcct cgctgagtaa tcattaatgc gcctgcgggc gcattaatca
    10201 cttcttttac ccccccccgt taattaactt cggcaaaaat atattt
//
LOCUS       DAZFLD010000168        10053 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.169, whole genome shotgun sequence.
ACCESSION   DAZFLD010000168 DAZFLD010000000
VERSION     DAZFLD010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10053)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10053)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10053
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.169"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..131)
                     /locus_tag="ACHKBL_003708"
     CDS             complement(<1..131)
                     /locus_tag="ACHKBL_003708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5820520.1"
                     /translation="MLQSSADKACNVLVKKVRYLNDVRKITKMMNGKLIYSWLVWPC"
     gene            209..1303
                     /gene="ddlA"
                     /locus_tag="ACHKBL_003709"
     CDS             209..1303
                     /gene="ddlA"
                     /locus_tag="ACHKBL_003709"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFO5820521.1"
                     /translation="MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGID
                     KQGQWHVSDASNYLLNADDPAHIALRPSATSLAQVPGKHEHQLIDAQNGQPLPTVDVI
                     FPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASAACMDKDVTKRLLRDAGLNIAPFIT
                     LTRANRHNISFAEVESKLGLPLFVKPANQGSSVGVSKVTSEEQYAIAVDLAFEFDHKV
                     IVEQGIKGREIECAVLGNDNPQASTCGEIVLTSDFYAYDTKYIDEDGAKVVVPAAIAP
                     EINDKIRAIAVQAYQTLGCAGMARVDVFLTPENEVVINEINTLPGFTNISMYPKLWQA
                     SGLGYTDLITRLIELALERHAADNALKTTM"
     gene            complement(1506..1718)
                     /gene="yaiZ"
                     /locus_tag="ACHKBL_003710"
     CDS             complement(1506..1718)
                     /gene="yaiZ"
                     /locus_tag="ACHKBL_003710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2754 domain-containing protein"
                     /protein_id="HFO5820522.1"
                     /translation="MNLPVKIRRDWHYYAFAIGLIFILNGVVGLLGFEAKGWQTYAVG
                     LVTWVISFWLAGLIIRRRDEETENAQ"
     gene            1978..2286
                     /gene="yaiY"
                     /locus_tag="ACHKBL_003711"
     CDS             1978..2286
                     /gene="yaiY"
                     /locus_tag="ACHKBL_003711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiY family protein"
                     /protein_id="HFO5820523.1"
                     /translation="MADFTLSKSLFSGKYRNASSTPGNIAYALFVLFCFWAGAQLLNL
                     LVHAPGVYERLMQVQETGRPRVEIGLGVGTIFGLIPFLVGCLIFAVVALWLHWRHRRQ
                     "
     gene            complement(2345..3439)
                     /gene="yaiW"
                     /locus_tag="ACHKBL_003712"
     CDS             complement(2345..3439)
                     /gene="yaiW"
                     /locus_tag="ACHKBL_003712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed outer membrane lipoprotein YaiW"
                     /protein_id="HFO5820524.1"
                     /translation="MSRVNHLSSLSLLAVLVLAGCSSQAPQPLKKGEKAIDVASVVRQ
                     KMPASVKDRDAWAKDLATTFESQGLAPTLENVCSVLAVAQQESNYQADPAVPGLSKIA
                     WQEIDRRAERMHIPAFLVHTALKIKSPNGKSYSERLDSVRTEKQLSAIFDDLISMVPM
                     GQTLFGSLNPVRTGGPMQVSIAFAEQHTKGYPWKMDGTVRQEVFSRRGGLWFGTYHLL
                     NYPASYSAPIYRFADFNAGWYASRNAAFQNAVSKASGVKLALDGDLIRYDSKEPGKTE
                     LATRKLAGKLGMSDSEIRRQLEKGDSFSFEETALYKKVYQLAEAKTGKSLPREMLPGI
                     QLESPKITRNLTTAWFAKRVDERRARCMKQ"
     gene            complement(3452..4672)
                     /gene="sbmA"
                     /locus_tag="ACHKBL_003713"
                     /pseudo
     CDS             complement(3452..4672)
                     /gene="sbmA"
                     /locus_tag="ACHKBL_003713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308454.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
     gene            5024..6181
                     /gene="ampH"
                     /locus_tag="ACHKBL_003714"
                     /pseudo
     CDS             5024..6181
                     /gene="ampH"
                     /locus_tag="ACHKBL_003714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308453.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine-
                     carboxypeptidase/endopeptidase AmpH"
     gene            complement(6182..6805)
                     /gene="iprA"
                     /locus_tag="ACHKBL_003715"
     CDS             complement(6182..6805)
                     /gene="iprA"
                     /locus_tag="ACHKBL_003715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen peroxide resistance inhibitor IprA"
                     /protein_id="HFO5820525.1"
                     /translation="MLSVVKPLQEFGKLDKCLSRYGTRFEFNNEKQVIFSSDVNSEDT
                     FVILEGVISLRREENVLIGITQAPYIMGLADGLMKNDIPYKLISEGSCAGYHLPAKQT
                     ITLIEQNQLWRDAFYWLAWQNRILELRDVQLIGHNSYEQIRATLLSMIDWNEELRSRI
                     GVMNYIHQRTRISRSVVAEVLAALRKGGYIEMNKGKLVAINRLPSEY"
     gene            complement(6891..9839)
                     /gene="ehaB"
                     /locus_tag="ACHKBL_003716"
     CDS             complement(6891..9839)
                     /gene="ehaB"
                     /locus_tag="ACHKBL_003716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin EhaB"
                     /protein_id="HFO5820526.1"
                     /translation="MHSWKKKLVVSQLALACTLAITSQANAATNDISGQTYNTFHHYN
                     DATYADDVYYDGYVGWNNYAADSYYNGDIYPVINNATVNGVISTYYLDDGISTNTNAN
                     SLTIKNSTIHGMIYSECMTTDCADRADDYYHDRLALTVDNSTIDDNYEHYTYNGTYNN
                     AADTHVVNVFNIGTAITLDQEVDLSISNNSHVAGITLTQGYEWEDIDDNTVSTGVNSS
                     EVFNNTITVKDSTVTSGSWADEGTTGWFGNTGNASDYNGDGWNADDVALAVIAHPYAD
                     NAMQTTATFDNSTLMGDVFFSSNFDENFFPQGRDSYRDADGDVDTNGWDGTDRLDLTL
                     NNGSKWVGAAMSAHQIGVDTDDDGVNDSYTYGINTEATATLIDIAANSLWPSSTVGVE
                     NSDSEYSEFDHIIGNEVYQSGLFNVTLNTGSQWDTTKTSLIDTLSINSGSTVNVADST
                     LISDSISLTGLSALNINEDGHVATDSLTVDNSTVTISDEVSAGWAVGDAALYANNIKV
                     TNDGILDVGNTAANALQVDTLNLTSTTDTSGNIHAGVFNIESNRFVLDADLTNDRTND
                     TTKSNYGYGLIAMNSDGHLTINGNGDNDNTASIEAGQNEVDNNGDHVAAATGNYKVRI
                     DNATGAGSIADYNGNELIYVNDKNSNATFSAANKADLGAYTYQAEQRGNTVVLQQMEL
                     TDYANMALSIPSANTNIWNLEQDTVGTRLTNSRHGLADNGGAWVSYFGGNFNGDNGTI
                     NYDQDVNGIMVGVDTKIDGNNAKWIVGAAAGFAKGDMNDRSGQVDQDSQTAYIYSSAH
                     FANNVFVDGSLSYSHFNNDLSATMSNGTYVDGSTNSDAWGFGLKAGYDFKLGDAGYVT
                     PYGSVSGLFQSGDDYQLSNDMKVDGQSYDSMRYELGVDAGYTFTYSEDQALTPYFKLA
                     YVYDDSNNDNDVNGDSIDNGTEGSAVRVGLGTQFSFTKNFSAYTDANYLGGGDVDQDW
                     SANVGVKYTW"
BASE COUNT         2595 a         2501 c         2393 g         2564 t
ORIGIN      
        1 ctgcatggcc atacaagcca ggaataaatt agcttcccgt tcatcatttt tgtgatcttg
       61 cgcacatcat tgagataacg cactttcttc accagtacgt tgcacgcttt gtcagcagaa
      121 gattgcaaca ttctgtcaga aggccgcgaa tcccccgttt tgtcgtggta atgtatgcac
      181 tttgtgttag acgggatagg tttttaagat ggaaaaactg cgggtaggaa tcgtttttgg
      241 tggtaaatca gcggaacatg aagtgtctct gcaatcggca aaaaacattg tcgatgccat
      301 tgataaaagt cgcttcgacg ttgtgctgct gggcattgat aaacaagggc aatggcacgt
      361 cagcgatgcc agcaattatc tgctaaatgc agacgatcct gcccatattg cgttgcgccc
      421 ttcggcgacc agccttgcgc aagtgccagg taaacatgag catcagctta tcgacgcgca
      481 aaacggtcag ccgttgccga cggtggatgt cattttcccg attgtccacg gtacgctggg
      541 cgaagatggt tccttgcagg gaatgctgcg ggtcgccaat ttaccgtttg taggttctga
      601 tgttctggct tcagcagcct gtatggacaa agatgtcacc aaacgtctgc tgcgcgatgc
      661 cgggctgaac attgcgccat ttattaccct gacgcgcgct aatcgtcaca acatcagttt
      721 tgccgaagtg gagtctaaac tggggttacc gctgtttgta aaaccggcta atcagggctc
      781 ttctgttggt gtcagcaaag taaccagcga agaacagtac gcaattgccg tcgatctggc
      841 gttcgagttc gatcataaag tgatcgttga gcaagggata aaaggtcgtg agatcgaatg
      901 cgcagttctg ggcaacgaca atccgcaagc cagcacctgt ggcgagatcg tactcaccag
      961 cgatttctat gcctacgaca ccaagtacat tgacgaagat ggcgcgaaag tggtagttcc
     1021 ggcagccatt gcgccagaaa tcaacgataa gatccgggcg attgccgttc aggcttatca
     1081 gacgttggga tgcgcaggca tggcgcgtgt agacgtgttt ttaaccccag agaacgaagt
     1141 ggtgatcaac gagatcaaca cactgcctgg cttcaccaac atcagtatgt atccgaagct
     1201 gtggcaagcc agcggtctgg gttacaccga cctgatcacg cgtttgattg agctggcgct
     1261 ggagcgtcac gctgcggata acgcattgaa aaccacaatg taatttttaa atgcctgata
     1321 acttttattg ggcatttaag actgatggca aacgtaagat tgcctgatgc gctttgctta
     1381 tcaggcctac cagataattg caatatactg attttgcatg attttgtagg cagggtaagg
     1441 cgttcgcgcc gcatccggca aaaacaacga gcactttgtc agcccccaga acgcccaatc
     1501 ctctgttatt gggcgttttc agtttcttca tcgcgacgac gaataatcag ccccgccagc
     1561 cagaaactaa tcacccacgt caccagaccg acggcatagg tctgccaacc ttttgcttca
     1621 aatcccagta aacccaccac gccattaaga atgaatataa ggccaatggc gaacgcatag
     1681 tagtgccagt cacggcggat ttttacaggc aagttcatgg cagctccgtt aacgaaaaac
     1741 aacatccttg catattctgc aacatccgtc tgtggcgtag acaacatttc cgtaattgtt
     1801 aataaattta acttaaggtt gtcattttgt gactcacgac gaagattcac gcttcgggaa
     1861 tattcccaac atcaatttac catgattctg atattgacaa tcttaatgat ctgtcagact
     1921 tgccataagc cgctggtatc acaccagaag agaagcagat gacttccgga ggtttttatg
     1981 gctgatttca ccctgtcaaa atcgctgttt agcggaaaat atcgcaatgc ctcttcaacg
     2041 cctggcaaca ttgcctatgc gttgtttgtg ctgttttgct tttgggctgg ggcgcaattg
     2101 ctgaacctgt tagtgcatgc gcccggcgtc tatgagcgct taatgcaggt ccaggaaaca
     2161 ggtcgcccac gggtggaaat tggtttaggt gtcggcacca ttttcgggct gatcccgttt
     2221 ttagttggct gcctcatttt tgcagtagtg gcgctatggc tgcactggcg acatcgccgc
     2281 cagtaagatt tacgttgcca gatgcggcct aaaacgcctt atctggcatg acattcctgt
     2341 ttgatcactg tttcatacaa cgcgcccgcc gttcgtctac gcgcttcgca aaccaggccg
     2401 tagtcaggtt gcgggtgatt ttcgggcttt ccagttgaat gccaggcaac atttcgcggg
     2461 gtagtgattt acccgttttc gcttcggcaa gttgataaac tttcttgtac agcgccgtct
     2521 cctcaaaaga gaagctgtcc cctttctcta actggcggcg aatttcactg tcgctcatcc
     2581 ccagttttcc cgccagtttg cgcgttgcca gttcggtttt cccgggctct ttactgtcgt
     2641 aacgaattaa atcaccgtcc agtgccagct tcacgccgct ggccttactg accgcgttct
     2701 gaaacgcggc attacggctg gcgtaccagc cagcgttaaa atcagcaaaa cggtatatcg
     2761 gcgcgctata gctggcggga taattcagta aatggtaagt accaaaccac aacccgccgc
     2821 gacgactgaa gacttcctga cggactgtac cgtccatttt ccacggatac cctttggtat
     2881 gctgttcggc aaaggcaatg ctgacctgca tcggcccacc ggtgcgcacc ggattaagcg
     2941 aaccaaacaa cgtctgcccc attggcacca tgctgatcag atcgtcaaaa atcgcgctca
     3001 attgcttttc agtacgcacc gaatctaacc gttcgctata gcttttgccg tttggcgatt
     3061 tgatttttag tgcggtatgc accagaaaag caggaatgtg catccgttca gcacgacggt
     3121 caatctcttg ccaggcgatc ttgcttaagc ccggaacagc cggatctgcc tgataatttg
     3181 actcctgctg cgccaccgcc agcaccgagc agacgttttc cagcgtcggt gccagcccct
     3241 gactttcaaa cgtggtggcg agatctttcg cccaggcatc gcggtctttc acgctcgcag
     3301 gcattttctg ccgcacgacg ctcgccacat ctatcgcttt ttcgcccttt ttcaacggtt
     3361 gcggagcctg actactacat cccgctaaaa ccagaaccgc caacaatgac agcgaagata
     3421 gatgattgac gcgcgacatt acttctcctt tttagctcaa ggtatgggtt acttcctgaa
     3481 ttttgtcacc atccagctca tgttcaaagc tgcgcagacg tttgtagata gacatcaact
     3541 caaccagtgt ggtccatgag ttaatcaggt actggaaagc accgcgaacc tgaccaaaaa
     3601 cgttagtaat ctgcgtcatc aggccgagtg taatcgtacc ggcaacaatt gacggaaaca
     3661 gcaagaacaa accgaaaacg ttatcgacct gcaaatagag aatgcgggcg atgttgaaat
     3721 acatatagtg aaaatagagg cggaaatagt ttttccgtac ggcgctaaac agctcgcgta
     3781 ccgtaggcgg cgtcgcacgc gtggcatcgt cttcaccata aaccagctct ttacggtagg
     3841 cagcctctac tcgctggttt ttaaactcca gccccggcag tttgatccct accactgcca
     3901 gtaatccggt ccccatcagc gaccagacga tagcggcaat caccagacca tacggaatgt
     3961 gcccgacaat cggcagctcc ggcacatgtg cggagagcgt taccagcacc ggcaggaagg
     4021 cgatcaacgt catgatagcg ttgataaagc tgacgcccat attctccagc gttgaagcaa
     4081 aacgcatggt gtcttcctgc acacgctgcg cggccccttc gatatgacgc agttgttgcc
     4141 agttcgccat gtaatattcg ttcatcgccg tacgccagcg gaacacgtag tgactgacaa
     4201 agaagttgtt cagcacactg atcaccaccg cgatcagcgc aatccccaga aagactccca
     4261 cttcgcggta aaattgttcg atggtgactt tatgcggcga acttagcgcc gtttgaatca
     4321 gatcatagaa cggcgcatac caggcgttga cggcaacccc gacttccacc aaaaaccagg
     4381 tgacgaagat aatcagcgca gtaccgagta ttgaccagta ttgccaacgg tgcgggctat
     4441 agataaacca gaacaatgca aaaagtccta cgcaaacaat gtagtaagcg taaaaaatca
     4501 ggaaatccaa cgaccagaaa cgcgcggcgc taatcgggat ctgcccggaa gcgccggtga
     4561 tacgcgccac ccaatccccc ccaccggctt accagaagat aacggcaatc aatgcccaaa
     4621 caaaggccga gagaaaaaac gttcccggct ttgggaaaaa agacttaaac atatgcactc
     4681 ctgctaactt cttatcgttt taacaactgc tgtgtattaa ccgcatgacc gctacaaagc
     4741 gtcagccaaa tgaaaccgcc cattgtgacc catagcaagg cgcttagttc gccagcctga
     4801 gcataagaat tgtttcgacg gatttcacaa accaaagatt gtttacataa gcggattaac
     4861 acgcgcagga tcacaaatgc aggcattggt gagcaggaaa aagatcgtgt ttacgcgccg
     4921 ttacgttttt aacactatta ttgcactaat tcgccctttg caatccatca atgagtagta
     4981 taaatacgct cagttacctt cattcaatct atggacacca ccgttgaaac gtagtctgct
     5041 tttttctgcc gtgctgtgtg cggcgtcatt gacctccgtc catgcggcgc agccgatcac
     5101 tgaaccggag ttcgcctctg atattgtcga tcgttatgcc gatcatattt tttacggcag
     5161 cggtgccacg gggatggcgc tggttgttat cgacggtaac cagcgcgtct ttcgcagtta
     5221 tggcgaaacg cgacctggta ataacgttcg cccgcagctg gattcggtca tacgtattgc
     5281 ttccctcacc aagctaatga ccagtgagat gctggtgaaa ttgctcgact aggggaccgt
     5341 gaagttaaac gatccgctaa gtaaatatgc tccgccaggc gcacgcgtac cgacttacaa
     5401 tgggacgccc attacgctgg tgaatctggc aacccatacc agcgccctgc cccgtgaaca
     5461 gcccggtggc gcggcacatc gtccggtatt tgtctggcca acgcgcgagc aacgctggaa
     5521 atacctttct acggcgaagc tgaaagccgc gccaggcagt caggcggcgt actctaacct
     5581 cgcattcgac ctgctggcag atgcactggc gaatgcgtcc ggcaagcctt atacccagtt
     5641 gtttgaagag cagattaccc gtccattggg gatgaaagac accacctaca ccccctcacc
     5701 ggatcagtgc cgccgtttaa tggttgctga gcgcggtgcc agtccgtgca ataacacgct
     5761 ggcggcaatt ggcagcggcg gcgtctattc cacgccaggc gatatgatgc gctggatgca
     5821 gcagtatttg tcatctgatt tttatcagcg cagtaaccag gctgatcgca tgcagacgtt
     5881 gatttatcag cgcgtgcagt ttacaaaagt gattggcatg gatgtccccg gcaaagcgga
     5941 tgcgcttggt ttaggctggg tatatatggc cccgaaagag ggtcgtccag ggattattca
     6001 gaagacaggt ggtggtggcg gtttcattac ctatatggcg atgatcccgc agaagaacat
     6061 tggcgcattt gtggtggtca ctcgttcgcc attgacgcgc ttcaaaaata tgagcgatgg
     6121 catcaatgac ctggtgaccg agctaagcgg gaataaaccg ttggttatcc ccgcatcctg
     6181 attaatactc tgaaggcaaa cggttgatag cgaccagttt gcctttattc atttcgatat
     6241 agccgccttt acgcaaagca gcgagaactt ccgcaacgac agaacgcgat atacgtgtac
     6301 gttgatggat ataattcatc acaccaatac gtgatcgcaa ttcttcattc cagtcaatca
     6361 ttgataataa cgtcgcgcgg atttgttcgt aggaattatg cccaatgagc tgaacgtcgc
     6421 gtaattccag aattctattt tgccaggcta accagtaaaa agcgtctcgc cagagttgat
     6481 tttgttcaat aagagtaatg gtttgtttgg ctggtaaatg atatcccgca cagcttcctt
     6541 ctgatattaa tttgtatggt atatcgtttt tcattaaacc atcagccagc cccataatat
     6601 aaggagcctg ggtaataccg ataagtacgt tttcttctct acgcagagag ataacgccct
     6661 ctaaaataac gaaagtatct tcgctattga catcactgga aaatataact tgcttttcat
     6721 tattaaactc gaagcgcgta ccgtatctgg acaaacattt atcgagctta ccaaattcct
     6781 gaagaggttt aactacagat aacatttgtg cgtcctttgc agtaatgccc gtcaaatcct
     6841 tgacgggcat tatttagatt aaattaccta tttcttcgga gtgaagaata ttaccaggta
     6901 tatttaacac ccacattcgc ggaccagtct tgatctacgt caccaccacc gaggtagtta
     6961 gcatcggtat aggcgctgaa gttcttggtg aagctgaact gagtacccag accaacacgt
     7021 accgcagacc cttcagtacc gttatcgatg gaatcaccgt tcacatcgtt atcgttgtta
     7081 gagtcgtcgt agacgtaagc cagtttgaag tacggagtca gagcctggtc ttcgctgtag
     7141 gtgaaggtat aacctgcatc tacacccagt tcataacgca tgctgtcgta agactgacca
     7201 tcaactttca tgtcgttgct cagctggtaa tcatcaccag actggaacag accagaaacg
     7261 ctgccgtaag gagtcacgta acctgcatca cccagtttga agtcgtaacc ggctttcaaa
     7321 ccaaagcccc aagcgtcgga gttggtgctg ccgtcaacgt aagtaccgtt gctcatggtt
     7381 gcagacaggt cgttgttgaa gtgagagtag ctcaagctac cgtcaacaaa gacgttgttc
     7441 gcgaagtgag cagaagagta gatgtaggca gtctggctgt cttgatccac ctgaccagaa
     7501 cggtcattca tatcaccttt agcgaagcct gcagccgcac cgacgatcca cttagcgttg
     7561 ttaccgtcaa ttttggtatc aacaccgacc atgatgccgt taacatcctg atcatagttg
     7621 atggtgccgt tgtcgccgtt gaagttacca ccgaagtagc ttacccatgc gccgccgtta
     7681 tcagccagac catgacgaga gttggtcaga cgagtaccaa cggtgtcttg ttccaggttc
     7741 cagatattgg tgttcgcaga cgggatgctc agcgccatgt tagcgtagtc ggtcagctcc
     7801 atctgttgca gaacaacggt gttaccacgc tgctcagcct gataggtgta tgcacccagg
     7861 tcagctttgt tagcagcaga gaaggtcgcg ttgctgtttt tgtcgttgac gtagatcagc
     7921 tcgttgccgt tgtagtcagc gatagaacca gcaccagtag cgttgtcgat acgaactttg
     7981 tagttaccgg ttgcagctgc aacatggtca ccgttgttat caacttcgtt ctgaccagct
     8041 tcgatagaag ccgtgttgtc gttatcgccg ttaccgttaa tggtcaggtg accatcagag
     8101 ttcattgcga ttaagccata accgtagttt gacttggtag tatcgttggt acggtcgttg
     8161 gtcagatcgg catcgagtac gaagcggttg ctttcgatgt tgaacacacc tgcgtgaatg
     8221 ttaccactgg tatcagtagt gctggtcagg ttcagagtat caacctgcag agcattcgcc
     8281 gcagtgttac ctacatccag gataccgtcg ttagtcactt tgatgttgtt agcatacaga
     8341 gcagcatcac ctacagccca gccagcagaa acttcatcag aaatggttac ggtactgttg
     8401 tcgacagtca gtgaatcagt tgcaacatga ccatcttcgt tgatgttcag cgcagaaaga
     8461 ccagtcagag agatagagtc agaaatcagc gttgaatcag caacattaac agttgaacca
     8521 ctgttgatgc tcagagtatc aatcagagaa gttttggtgg tatcccactg tgaaccggtg
     8581 ttcagggtca cattgaacag accgctctgg taaacttcgt taccgatgat gtgatcaaat
     8641 tcgctgtatt cagagtcgct gttttctaca ccaacagttg atgaaggcca caggctgtta
     8701 gctgcgatgt cgatcagagt tgcagtggct tcagtattga ttccatatgt ataggagtca
     8761 tttacaccat cgtcatcagt atcaacacca atctgatgcg cagacattgc cgcaccgacc
     8821 cacttgctgc cattgttcag ggtcagatcc aggcgatcag taccatccca accgttagta
     8881 tctacatcac cgtcagcatc acgatagcta tcacgacctt gtgggaagaa gttctcatca
     8941 aaattacttg agaagaatac atcgcccatc agtgtggaat tgtcgaatgt tgcagtggtt
     9001 tgcatggcgt tatcagcata tggatgtgca ataacagcta atgcaacatc atcagcattc
     9061 cacccatcac cattgtaatc actagcatta ccagtattac caaaccaacc agtagtacct
     9121 tcatcagccc atgaaccaga ggtcacggta gaatctttaa cagtgatggt gttattaaat
     9181 acttcgctgc tgtttacacc agtgctgaca gtgttgtcgt cgatatcttc ccactcataa
     9241 ccctgagtca gcgtaatacc tgctacatga gagttattag agatggacag atcgacttcc
     9301 tgatccagag tgatggcagt accaatgtta aatacattta caacatgagt gtcagcggca
     9361 ttattgtaag tgccgttata tgtgtagtgt tcgtagttgt catcgatcgt tgaattatca
     9421 acggtcagcg ccagacgatc atggtagtag tcatccgcac gatcagcaca atcagttgtc
     9481 atgcactcgg aataaatcat accgtgaata gtgctgtttt tgattgtcag gctattggcg
     9541 ttggtattag tagaaatacc atcgtccaga tagtaggtag aaatcacgcc gttaacggta
     9601 gcgttattaa tgaccggata gatatcgccg ttgtaataac tatcagcggc atagttattc
     9661 cagcctacat aaccatcata gtaaacgtca tcagcatagg tggcgtcgtt gtagtgatgg
     9721 aaagtattgt aagtttgacc agaaatatcg ttggtcgctg cattagcctg agaggtgata
     9781 gccagagtgc aagccaatgc taattgtgat actacaagtt tctttttcca ggagtgcatt
     9841 tgtcatccct cctcagggac gtaataagtt gatccatcaa ttatcaatgc atagaaaaca
     9901 tattatgttt cgcgtttacg attatgcggc gttatagagg aatggttcaa ttattctttc
     9961 ccctaagtcc gattgcggac atatataaag cacgtgaaca cacacaaaga aagaaataac
    10021 aaaaacacaa tatttcaata cgttaacgaa agc
//
LOCUS       DAZFLD010000169         9984 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.170, whole genome shotgun sequence.
ACCESSION   DAZFLD010000169 DAZFLD010000000
VERSION     DAZFLD010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9984)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9984)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9984
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.170"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(141..1514)
                     /locus_tag="ACHKBL_003717"
                     /pseudo
     CDS             complement(141..1514)
                     /locus_tag="ACHKBL_003717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244358.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            complement(1713..2759)
                     /gene="fbpC"
                     /locus_tag="ACHKBL_003718"
     CDS             complement(1713..2759)
                     /gene="fbpC"
                     /locus_tag="ACHKBL_003718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308440.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric ABC transporter ATP-binding protein"
                     /protein_id="HFO5820527.1"
                     /translation="MSQKNFVELRNVTKRFGSNRVIDNINLTIPQGQMVTLLGPSGCG
                     KTTILRLVAGLEKPSEGQIFIDGEDVTHRSIQQRDICMVFQSYALFPHMSLGENVGYG
                     LKMLGVSRSEVKQRVKDALTMVDLEGFEDRYVDQISGGQQQRVALARALILKPKVLLF
                     DEPLSNLDANLRRSMRDKIRELQKQFNITSLYVTHDQSEAFAVSDTVLVMNKGHIMQI
                     GSPQDLYRQPASRFMASFMGDANLFPANFSEEYVDIYGYRLPRAAHFPAQGSGTAGVR
                     PEAITLNNHGEESQRCVIRHVAYMGPQYEVTVEWHGQEILLQVNATRLQPDIGEHYYL
                     EIHPYGMFVLADAA"
     gene            complement(2771..4849)
                     /locus_tag="ACHKBL_003719"
     CDS             complement(2771..4849)
                     /locus_tag="ACHKBL_003719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO5820528.1"
                     /translation="MSQTLALHPVKKRDAIFLWVLLGWLAFILLPSWSLDYGLLESTS
                     DEILEAYGWSHFNISWLWYLLPSLLLVRPFREATREQRSRHYFDASWALLCMAFIVIS
                     ATVAGRGLGYAAIVLFVELGAIMTLALTRLEWLGGDRFVIGSLIAIVALIGIFIVWPS
                     IAIFIPMFTNDAGEFAPLAFMNVLSQAHIVQVIVNSIVLSIAVGIGCTFFGLVLAIYT
                     TRIAKRSAIIGRIFSILPIVTPPFVVGLGVTLMMGRSGYITEFMVEWFGLTNTNWLYG
                     FTGIWLAQVLAFTPMAFMILDGAIKTIHPSLEEASYTLRASRWQTFNGVFVPLLKPAL
                     ANAFLIVVVQSLADFSNPLVLGGNFDVLATQIYFYITGSQLDYQAASTLGAFLLLFSL
                     LVFCVQYMWIGKRSYVTVSGKSYRGDVQPLPVTLVWSVVAILTVWIAFNALLYGSIFY
                     GSFTVNWGVDYTLTLDNFIKLFGQGMSDGAWPSLLDTLLYAGIAAPITAIFGLLIAWV
                     VVRQQFKGKKTIEFTTMLCFAVPGTVAGVSYILAFNSAPLYLAGTAAIVIISMVMRNV
                     PVGIRAGIAGLGQIDKSLDEASLSLRAGSLRTITHILLPLLRPAILSALIYSFVRAIT
                     TVSAIVFLVTPDTRVATAYILNRVEDGEYGVAIAYGSILIVVMLAIIFLFDWLIGEAR
                     ISRSKAKNQA"
     gene            complement(4918..5949)
                     /locus_tag="ACHKBL_003720"
     CDS             complement(4918..5949)
                     /locus_tag="ACHKBL_003720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFO5820529.1"
                     /translation="MKMTFTSTLIASAVALATLTGAAQAKGRLVVYCSATNEMCEAET
                     KAFGEKYDVKTSFIRNGSGSTLAKVDAEKKNPQADVWYGGTLDPQSQAGEMGLLQAYK
                     SPNLEQIMEKFRDPAKVKGNLSSAVYVGILGFGVNTDRLKEKNLPVPKCWKDLTKPEY
                     KGEIQIADPQSSGTAYTALATFAQLWGEDQAFDYLKQLNANVSQYTKSGIAPARNAAR
                     GETAIGIGFLHDYSLEKEQGAPLELISPCEGTGYEIGGVSILKGARNLDNAKLFVDWV
                     LSKEAQELAWKKGKSYQILTNTTAETSPNSLKLDDLKLINYDMDKYGSTDVRKALINK
                     WVSEVKMGK"
     gene            complement(5946..7262)
                     /gene="uhpC"
                     /locus_tag="ACHKBL_003721"
     CDS             complement(5946..7262)
                     /gene="uhpC"
                     /locus_tag="ACHKBL_003721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001312597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HFO5820530.1"
                     /translation="METTMHARSAREINQCYRTLRPRLLMCMIIGYAAFYLTRKSVNY
                     VLPALQTDLGLDKGDIGLLGSLFYLSYGLSKFTAGLWHDSHGQRGFMGVGLFATGLLN
                     VVFAFGESLTLLLVVWTLNGFFQGWGWPPCARLLTHWYSRNERGFWWGCWNMSINIGG
                     AIIPLISAFAAHWWGWQAAMLTPGIISMALGIWLTLQLKGTPQEEGLPTVGHWRHDPL
                     ELRQEQQSPPMGLWQMLRTTMLQNPLIWLLGVSYVLVYVIRIALNDWGNIWLTESHGV
                     NLLNANATVMLFEVGGLLGALFAGWGSDLLFSGQRAPMILLFTLGLMVSVAALWLAPV
                     HHYALLAVCFFTVGFFVFGPQMLIGLAAVECGHKAAAGSITGFLGLFAYLGAALAGWP
                     LSLVIERYGWSGMFSLLSVAAVLMGLLLMPLLMAGITTTHARRIKQ"
     gene            complement(7335..8876)
                     /locus_tag="ACHKBL_003722"
     CDS             complement(7335..8876)
                     /locus_tag="ACHKBL_003722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
                     /protein_id="HFO5820531.1"
                     /translation="MSRNLRHWVISLFIVLAWGTGWLMLWTLSFYLTNNGQQAVLFLP
                     QGVYLALLILLSRRYWPALVLPPLLMMFWLHSEQLLNGYLMLATPVISLFPAILAQNF
                     WHRFPLYWQRLTLLLATVTAASLLNTALLSPFMSGPIMLPGLTSFTGGVLLTPFVYLI
                     FEFLRQQHRYQLLGLDTHNPPLRTSLIIWCSLFFIIGIGTQMVLSPEIERLLLIVVFL
                     PNVVMAWKFGWQGGVLSGLLGSMMITIARQIGVGFSNLVELEIFLATQALLGIGLGIA
                     ISRQQHLALNLHHYRQRLEAELAARRALAEKLIHTEEDTRKNLARELHDEIGQNITAI
                     QIQSQLVKRARDPAQIQSAASQINELARRIHLSTRQLLRQLRPPALDELTFREALLHL
                     INEFAFSERGIHCQFAYQLNSTPENETVRFTLYRLLQELLNNVCKHAEASEVTIILRQ
                     QGEVLHLEVSDNGVGIASGKMAGFGIQGMRERVSALGGDLTLEKQHGTRVIVNLPTFL
                     QQIAG"
     gene            complement(8876..9505)
                     /locus_tag="ACHKBL_003723"
     CDS             complement(8876..9505)
                     /locus_tag="ACHKBL_003723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFO5820532.1"
                     /translation="MIRVVLVDDHVVVRSGFAQLLSLEDDLEVIGQYSSAAQAWPALI
                     RDDVNVAVIDIAMPDENGLSLLKRLRAQKPQFRAIILSIYDAPTFVQSALDAGASGYL
                     TKRCGPEELVQAVRSVGLGGHYLCADAIRALRGGGQPAQALEILTPREREVFDLLVKG
                     DSVKEIAFKLELSHKTVHVHRANVLGKLNCHSTIELVHFALDHHLLAGH"
     gene            complement(9781..>9984)
                     /locus_tag="ACHKBL_003724"
     CDS             complement(9781..>9984)
                     /locus_tag="ACHKBL_003724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO5820533.1"
                     /translation="ATGHGFRHTMSTILHEQGFNSAWIEMQLAHVDKNSIRGTYNHAL
                     YIDGRREMMQWYADYIDSLSEQT"
BASE COUNT         2480 a         2707 c         2596 g         2201 t
ORIGIN      
        1 ttatgtcata acttttctga aagatagctg gtaattgcct tgagaaagtt tttatatgtt
       61 attctgtaaa acaggataac agaaaataat tatgtctgaa ataagtagat gatgtcccct
      121 ctgagctggc tgtctcttga tcagatatcc tgatcaagag acttcatcac caggtaaccc
      181 tcaaccatat cctgaagtct gaaccagtca tcccacataa ctacccaacc ggggcggcct
      241 gtgcgtttgc tgtcatgtca tcgccccagt ttagccagtt tcagactggc tcatttcagg
      301 atcggtgcct gcgccggaag cggctctcct tccagcttaa cccacagcag tttccactct
      361 gtcggcgtca gtattttctc acaactgtca ttttgtgttt cttcactgac acctccctgc
      421 cgcaggcccg gcacccgtac cgcgataaac gccttgataa ccaccatgcg ctcaaggtta
      481 tcccgggtct gaattcgcag cgattccaca catgtaccac cacttttcca cgccttgtgg
      541 tattcctcta tcagccagcg tcgctcgtaa tggctgacga tacgtcgcgc atcggcggca
      601 ctcgccactt tttctgacgt cagcagatgc cagcaggcac tgtcctctgc ctgctcccgg
      661 caacagacat acgtgagcga gagcgcctgg ccgctgttgt cgggattttt tatgctgact
      721 tcgctgtaac tgatgaacat ccgggcctgg cgggctgccc gcccgccttt ttgcatcaca
      781 ttcagcgtgt ggcttcccgc ggttgccagg acttccggca gttcgaagag cttgccgggt
      841 gcttcttcca gccggctatt ctgtgcagca cgcaccacga agcgctgtcc gtggctgact
      901 ttataatgca ggtaatgcca gatatccgct tcccggtcac agacagtgat tacccgtttc
      961 tgtatctccc ccagccgttc ggccatgcgc tccgaagcct gctgccagcg gtaactttct
     1021 ttttcttcac agggacgttc ttttcgctgg tgcttaacac cgtaggtgtc cgtggcccga
     1081 ctccagctct gctgttcgat aagagcgact ggcagggcgc tgtcgggggc gtatatcagg
     1141 acagagtgag ccagcagccc gcgcgtcttc gggttagtgg tggtattccc caggtcatca
     1201 gatgccgtac tgtggctgaa gttaatggtg gaggtgtctt ccagtgcggg gagcagcgga
     1261 tgtgcctcac atgcccttgc agtggcggta aatccggctc cggaaatggc ttccggggac
     1321 actgacgggt tacgcatcag gcggtacgca ccttcaacct gagcagtgga gtgggattat
     1381 ttcacaatag aaagacctgc atgctgagcg agagaagagg ccagtgacac aaggcgtcgt
     1441 gtacgacgcg gatcaccgag acgggcatgt ccagactgct cgttagccca tgaataacaa
     1501 tcagaaagta ccataacaga cccgaagaaa atgaaatata agagaagatc aacgggtgaa
     1561 gaaaaagttc aaaaaatcgc tgccggggag gaaggaaact accggatgga aagagtcccc
     1621 cctaaagcag actgacagac atcacaaatc cccaggtggt gatttgtgta taagagacag
     1681 ctgagctgga ggggaccgct tatgcgctat ttttatgccg catccgctaa aacaaacatc
     1741 ccgtaagggt ggatttcgag gtaatagtgc tcaccaatat cgggctgtaa acgggtggcg
     1801 ttgacttgca ataaaatctc ctgaccatgc cattctacgg tcacttcgta ctgcggcccc
     1861 atgtaagcga catggcgaat aacacaacgc tgactctctt cgccgtgatt gtttaaggtg
     1921 atggcttccg ggcgaacgcc ggcggtgccg ctaccttgcg ccgggaaatg cgctgcgcgc
     1981 ggcaggcgat aaccgtagat atcgacatac tcttcgctaa agttcgccgg gaacaggttg
     2041 gcgtcgccca taaaacttgc cataaatcgg gaggctggct gacgatagag atcctgcggt
     2101 gagccaatct gcatgatgtg acctttattc atcaccagca cagtgtcgga caccgcgaaa
     2161 gcttcacttt gatcgtgagt cacgtagagt gacgtgatat taaactgctt ttgcagctcg
     2221 cggatcttat cgcgcatgct gcggcgcagg ttggcatcga ggttacttaa cggctcatca
     2281 aacagcagca cctttggctt gaggatcaac gcacgggcca gagccacacg ctgttgctga
     2341 ccaccggaaa tctggtcgac atagcggtct tcgaatcctt ccagatccac cattgtcagc
     2401 gcatctttca cccgctgctt cacttcacta cgcgacacgc caagcatttt cagcccatag
     2461 ccaacgtttt cgcccagcga catatgcggg aacagagcgt atgactgaaa caccatgcag
     2521 atatcgcgtt gctgaatgga acgatgcgtg acatcttcgc catctataaa aatttgccct
     2581 tcgctcggtt tttccagccc ggcaaccaga cgcagaatgg tggttttgcc gcatccggaa
     2641 ggaccgagca gcgtcaccat ttgcccttgt gggatggtga gattgatatt gtcgataacc
     2701 ctattactgc cgaatcgttt agtgacgttg cgcagttcca caaaattttt ctgactcata
     2761 gtgcgctcca ttacgcctgg tttttggctt tagaacggga aatacgtgct tcaccgatca
     2821 gccagtcaaa gaggaaaata atcgccagca tcaccacgat cagaatcgaa ccgtaggcga
     2881 tggctacgcc atattcgccg tcttctacgc ggttaaggat gtaggcggtt gccacgcgtg
     2941 tgtccggtgt gacgagaaag acgatggcgc tgactgtagt gatggcccgc acaaagctgt
     3001 aaatcagggc cgagagaatg gccgggcgta acagcggcag taaaatgtgc gtaatggttc
     3061 gcagggaacc cgcacgcagg ctaagtgagg cttcatccag cgatttgtcg atctgcccca
     3121 gacctgcaat cccggcgcga atacccaccg gaacgttacg catcaccatc gagataataa
     3181 caattgctgc cgttccggcg agatagagtg gggcgctatt aaaggcaaga atatacgaga
     3241 cacccgccac tgtacccggt acggcaaagc acagcatggt ggtgaactcg atggtctttt
     3301 tgcctttgaa ttgttgacgc accaccaccc aggcgatgag caggccaaag atagcggtaa
     3361 tgggggctgc aatcccggcg tagagcaggg tatccagcag tgaaggccac gcgccgtcgc
     3421 tcatcccctg accaaataac ttgataaagt tatccagcgt cagggtgtag tcaacgcccc
     3481 agttcaccgt gaagctgccg tagaaaatgc tgccgtagag cagggcgtta aaggcaatcc
     3541 ataccgtaag tatggcgacc acgctccaca ccagggtgac cggtagcggc tggacgtcgc
     3601 cacggtagga tttgccggaa accgtgacgt aagagcgttt accaatccac atatactgga
     3661 cgcagaacac cagcagcgag aacagcagca ggaaagcacc aagcgtgctg gcggcctgat
     3721 aatccagctg cgagccggta atgtagaagt aaatttgcgt cgccagtacg tcgaagttgc
     3781 cgccaagcac cagcgggtta ctgaaatcag ccagtgactg caccacgacg attaaaaagg
     3841 cattggcgag cgcgggcttt agcaatggca caaacacgcc gttaaaggtt tgccagcggc
     3901 tggcgcgcag ggtataggag gcttcttcca gcgagggatg aatggtttta atggccccgt
     3961 cgaggatcat aaaagccatt ggcgtgaatg ccagaacctg cgccagccag atcccggtaa
     4021 aaccatacag ccagttggtg ttggtcagcc caaaccactc caccataaac tccgttatgt
     4081 agccggagcg ccccatcatg agcgtcacgc ctaagccgac cacaaaaggt ggcgtaacga
     4141 tgggcagaat ggaaaaaatg cggccaataa tggcgctgcg tttggcgatg cgcgtggtat
     4201 aaatcgccag caccaggccg aagaaggtac agccaatacc caccgctatc gacaagacga
     4261 tagagttgac gatcacctgc acaatatgtg cctgcgagag cacgttcata aacgccagcg
     4321 gggcgaactc tcccgcgtcg ttggtgaaca tcgggataaa aatggcgatg ctcggccaga
     4381 cgataaaaat gccaatcagc gccacaatcg caatcagtga gccgatcacg aagcggtcgc
     4441 cgcccagcca ttcgaggcgg gttaaggcca gcgtcataat cgcgcctaat tcaacaaaca
     4501 gcacgatggc ggcgtagcct aaaccgcgtc cagccacggt ggcgctgatg acgataaacg
     4561 ccatacagag caatgcccag ctggcatcaa aataatgacg actacgctgt tcgcgtgtgg
     4621 cctcacgaaa cggacgaacc agcaacagac tcggtaataa ataccacagc cagctgatat
     4681 taaaatgcga ccagccataa gcctcgagga tctcatcact cgtggactcc agcaggccat
     4741 aatcaaggct ccagctcggc aacaggataa atgccagcca gcccagcaaa acccaaagga
     4801 aaatcgcatc ccgctttttc acgggatgga gtgcaagtgt ctgtgacata gcagttccgg
     4861 tatggttggc cggggcaggc gtttgtgctg cacccggcaa atgcgcctaa tgcgggttta
     4921 tttacccatc ttcacttcgc tgacccattt attaatcaat gccttacgaa catccgtgga
     4981 accatattta tccatgtcgt agttgattaa tttcaggtcg tcgagcttca gcgaatttgg
     5041 cgacgtttcg gcggtggtat tggtcaggat ctgataagac ttccctttct tccacgccag
     5101 ttcctgagct tcttttgata atacccagtc cacaaacagt ttggcgttgt cgaggttgcg
     5161 cgcgcctttc agaatgctga cgccgccaat ttcgtagccc gtaccttcac acggggaaat
     5221 caactccagc ggcgcgccct gttctttttc cagcgagtag tcatgcagga agccaatacc
     5281 aatcgccgtt tcgccacggg cggcgttacg tgccggggct atgcctgatt tggtgtactg
     5341 ggagacgttg gcgttcagtt gttttagata atcaaaggcc tgatcttccc cccacagctg
     5401 cgcaaaagta gccagggcgg tgtaggcagt accggaactt tgcggatcgg caatttgaat
     5461 ttcgccttta tattccggtt tggtgagatc tttccagcac ttcggcaccg gcaggttttt
     5521 ctctttcaga cgatcggtgt ttacgccaaa accgaggatc cccacatata cggcagagga
     5581 cagattccct ttcacttttg ccggatcgcg gaatttctcc atgatctgct caaggttagg
     5641 ggatttatac gcctgtagca ggcccatctc gccagcctgc gattgcgggt ctaaagtgcc
     5701 gccgtaccag acgtcagcct gggggttttt cttttcagca tcgacttttg ccagggtgct
     5761 tccggaaccg ttgcggataa acgaggtttt tacatcatat ttctcgccaa acgctttggt
     5821 ttcggcctca cacatttcat tggtggcgct gcaatagacc accagccggc ctttcgcctg
     5881 cgcggcaccc gttaaagtcg ccagcgcaac ggcagaagca atcagagtag aggtaaaggt
     5941 cattttcatt gttttatcct tcgagcgtga gtggtggtga tgcccgccat cagtaacggc
     6001 atcagcagta aacccataag tactgcggcg accgagagta aactgaacat tcccgaccag
     6061 ccgtagcgtt cgatgaccag cgacagtggc cagcccgcaa gcgccgcgcc aaggtaagcg
     6121 aacaggccga gaaatccggt gattgaaccc gccgcagctt tgtgcccaca ctccaccgcc
     6181 gcaagtccaa tcagcatttg tggaccaaag acgaaaaagc ccacggtaaa aaagcacact
     6241 gccagcagag cgtagtgatg aaccggtgcc agccacagtg cggcgactga caccatcaac
     6301 cccagcgtga acagcaaaat cattggcgct cgttgcccgc taaacagcaa atccgatccc
     6361 catccggcaa ataacgcgcc gagtaagccg cccacttcaa acagcattac cgtggcgttg
     6421 gcgttgagca ggttgacgcc gtggctttcc gtcagccaga tattgcccca gtcgttaagc
     6481 gcaatccgga tcacataaac cagtacataa gagacgccca gtagccagat cagcggattt
     6541 tgcagcatgg tggtgcgcaa catttgccac agccccatcg gcggactttg ttgttcctga
     6601 cgtaactcca gcggatcgtg acgccagtga ccaactgtcg gtaatccctc ttcctggggc
     6661 gtgcctttga gttgtagcgt taaccagatc cctaatgcca tgctgattat ccccggtgtc
     6721 agcatcgcgg cctgccagcc ccaccagtgg gcggcaaagg cgctaatcag cggaataatc
     6781 gctccgccaa tgttgatcga catattccag cagccccacc agaaaccgcg ctcattgcgt
     6841 gagtaccagt gggtcagcaa gcgggcacac ggtggccatc cccagccctg aaaaaaaccg
     6901 ttcagtgtcc agaccacaag cagaagcgta agtgattcgc caaaggcaaa caccacgttc
     6961 aataacccgg tggcgaacaa gccgacgccc ataaaaccgc gttgtccgtg gctgtcatgc
     7021 cacagtccgg cggtaaattt cgacaatccg tagctgagat aaaacagcga acccagcagg
     7081 ccaatgtcgc ctttatccag tccgaggtcg gtttgcagcg ccggaaggac gtaattcacg
     7141 ctttttcgcg tcagataaaa tgcggcgtag ccaataatca tgcacatcag caatcgcgga
     7201 cgtaatgtcc ggtagcattg attaatttct ctcgctgaac gcgcgtgcat ggttgtctcc
     7261 atgttgctga ctatatgaaa atgaaagtga aaggggatga gagaaaatcc gggataccta
     7321 agactttttc ctggttatcc ggcgatttgt tgcaaaaatg tgggcaagtt aacaattacc
     7381 cgcgtgccgt gctgtttttc gagcgttaag tcgccgccca gggcgctgac gcgctcacgc
     7441 atcccctgaa tgccaaatcc ggccattttc ccgctggcga tccccacacc gttgtcgctg
     7501 acctccagat gcaatacctc tccctgctgg cgcaggataa tcgtcacttc gctggcttcg
     7561 gcgtgtttac agacgttatt gagcaactcc tgcaataacc gatacaaggt aaagcgcacc
     7621 gtttcgtttt ctggtgtgga gttcagttga taggcaaact ggcaatgaat gccgcgttca
     7681 ctaaaggcaa attcattaat cagatgcagc aacgcttcgc ggaatgtcag ttcatcaagc
     7741 gcgggaggcc gcaactggcg cagcaactgg cgggtggaga gatgaatgcg gcgggcgagt
     7801 tcattgattt ggctggctgc ggattgaatt tgcgccggat cgcgcgcccg cttcaccagt
     7861 tgcgactgga tctgaatggc ggtgatgttc tgaccgattt cgtcgtgcag ctctcgcgcc
     7921 aggtttttgc gcgtgtcctc ctcggtgtgg atcagctttt ccgccaatgc ccgccgcgct
     7981 gccagctcgg cctccagccg ttgtcggtaa tggtgcaggt ttagcgccag atgttgctgg
     8041 cgactgatgg caatgcccag gccaattccc agcaatgcct gggtcgcgag gaaaatctcc
     8101 agctccacca ggttactaaa gcccacgcca atctggcggg cgatggtgat catcatgctg
     8161 cccaaaagcc cggagagtac gccaccttgc cagccaaatt tccacgccat caccacattg
     8221 ggtagaaaaa ctacgatcaa cagcaggcgt tcaatttcag gtgacagcac catctgagtg
     8281 ccgataccaa tgataaaaaa caggctacac cagatgatta aagaggtacg caagggggga
     8341 ttatgcgtgt caagccccag gagctggtag cgatgctgct ggcgcagaaa ctcaaagatg
     8401 agatagacaa acggcgtcag taatacgccg ccggtaaatg aggtcaaccc cggcagcatt
     8461 attgggcctg acataaaagg ggagagcagg gcggtattca acaatgaagc tgccgtgaca
     8521 gtggctaaca gcaacgtcag acgctgccag taaagtggaa aacggtgcca gaagttttgc
     8581 gcaaggatgg caggaaacag gctaataacc ggcgttgcca gcatgagata accgttcaat
     8641 agctgttcac tatggagcca gaacatcatc agcaaaggtg ggagcaccag cgcaggccag
     8701 tagcggcgag agagcaaaat aagaagggcg aggtaaaccc cctgcgggag aaacaacacc
     8761 gcctgttgac cgttattggt cagataaaag ctgagcgtcc acagcatcag ccagccagtt
     8821 ccccaggcca gcacaataaa cagagaaatc acccaatgac gaaggttgcg cgacattaat
     8881 gccccgccag cagatggtgg tcgagcgcaa aatgcaccag ctcaatggta ctatggcagt
     8941 tcagttttcc cagcacattg gcgcgatgca catgtaccgt tttatgactg agttcgagct
     9001 taaaggcgat ctctttaacg ctgtcacctt tgaccagtaa atcaaacact tcacgttctc
     9061 gtggggtgag aatttccagc gcctgggcgg gttgcccgcc gccgcgtaaa gcccgaattg
     9121 catcggcaca gagataatgc ccgcccagcc cgaccgagcg caccgcctgt actaactctt
     9181 ccggcccaca gcgtttggtc aaatagccgc tggcaccagc atccagcgcg ctttgcacaa
     9241 aggttggcgc gtcataaatg ctaagaataa tggcgcgaaa ttgcggtttt tgtgcacgca
     9301 ggcgcttaag caaactgagg ccgttttcgt ccggcatagc aatatcaatg acggcaacat
     9361 tgacgtcatc acggattaat gccggccatg cctgtgcggc actactgtac tgaccaatga
     9421 cctccagatc gtcttccaga gagagtaatt gtgcgaagcc agagcgcacc accacatggt
     9481 catccaccag cacaacccga atcatacttt tcttctctta ttatctcatt cttctatcat
     9541 gcatcacggc gcgtcgctgg caatcaacca gcgacagata tggaatggat gtaacaaaaa
     9601 atgtaatttg ttattcaata taattatgct ttatttcatg agtggctcta tatctggtgt
     9661 tcagattatt gtctgcaggg gcagttcaga agatgtgagc aaataaaaaa gaaaacataa
     9721 ataaaaccat ccggctttcg gggccagatg gttattcatt atttttatgt cactgattca
     9781 tcaggtttgc tcagaaagcg aatcaatgta gtcagcatac cactgcatca tttcacggcg
     9841 accatcgata tacagggcat gattataggt acccctgatg gaatttttat ccacatgagc
     9901 taactgcatt tcaatccagg cagaattaaa cccctgttcg tgcagaatgg tactcatggt
     9961 gtgacgaaaa ccatgaccgg ttgc
//
LOCUS       DAZFLD010000170         9932 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.171, whole genome shotgun sequence.
ACCESSION   DAZFLD010000170 DAZFLD010000000
VERSION     DAZFLD010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9932
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.171"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..582)
                     /gene="yeeO"
                     /locus_tag="ACHKBL_003725"
     CDS             complement(<1..582)
                     /gene="yeeO"
                     /locus_tag="ACHKBL_003725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001323172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
                     /protein_id="HFO5820534.1"
                     /translation="MWFHFLQLRSALNISSALRQVVHGTRWHSKRKSYKVLFWREITP
                     LAVPIFMENACVLLMGVLSTFLVSWLGKDAMAGVGLADSFNMVVMAFFAAIDLGTTVV
                     VAFSLGKRDRRRARVATRQSLVIMTLFAVLLATLIHHFGEQIIDFVAGDATTEVKALA
                     LTYLELTVLSYPAAAITLIGSGALRGAGNTKIPL"
     gene            743..818
                     /locus_tag="ACHKBL_003726"
     tRNA            743..818
                     /locus_tag="ACHKBL_003726"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:776..778,aa:Asn,seq:gtt)
     gene            complement(856..1806)
                     /gene="cbl"
                     /locus_tag="ACHKBL_003727"
                     /pseudo
     CDS             complement(856..1806)
                     /gene="cbl"
                     /locus_tag="ACHKBL_003727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310810.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator Cbl"
     gene            complement(1908..2825)
                     /gene="nac"
                     /locus_tag="ACHKBL_003728"
     CDS             complement(1908..2825)
                     /gene="nac"
                     /locus_tag="ACHKBL_003728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen assimilation transcriptional regulator
                     NAC"
                     /protein_id="HFO5820535.1"
                     /translation="MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVATLEGELN
                     QQLLIRTKRGVTPTDAGKILYTHARAILRQCEQAQLAVHNVGQSLSGQVSIGFAPGTA
                     ASSITMPLLQAVRAEFPEVVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGVSSQA
                     LLKEDLFLVGTQDCPGQSVDVNAIAQMNLFLPSDYSAVRLRVDEAFSLRRLTAKVIGE
                     IESIATLTAAIASGMGVAVLPESAARSLCGAVNGWMSRITTPSMSLSLSLNLPARANL
                     SPQAQAVKELLMSVISSPVMEKRQWQLVS"
     gene            3152..3227
                     /locus_tag="ACHKBL_003729"
     tRNA            3152..3227
                     /locus_tag="ACHKBL_003729"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3185..3187,aa:Asn,seq:gtt)
     gene            complement(3282..4217)
                     /gene="ldtA"
                     /locus_tag="ACHKBL_003730"
     CDS             complement(3282..4217)
                     /gene="ldtA"
                     /locus_tag="ACHKBL_003730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFO5820536.1"
                     /translation="MMRRVNILCSFALLFASQNSLAVTYPLPPEGSRLVGQSLTVTVP
                     DHNTQPLETFAAQYGQGLSNMLEANPGADVFLPKSGSQLTIPQQLILPATVRKGIVVN
                     VAEMRLYYYPPDSNTVEVFPIGIGQAGRETPRNWVTTVERKQEAPTWTPTPNTRREYA
                     KRGESLPAFVPAGPDNPMGLYAIYIGRLYAIHGTNANFGIGLRVSQGCIRLRNDDIKY
                     LFDNVPVGTRVQIIDQPVKYTTEPDGSKWLEVHEPLSRNRAEYESDRKVPLPVTPSLR
                     AFINGQEVDVNRANAALQHRSGMPVQISSGSRQMF"
     gene            complement(4279..5358)
                     /gene="cobT"
                     /locus_tag="ACHKBL_003731"
     CDS             complement(4279..5358)
                     /gene="cobT"
                     /locus_tag="ACHKBL_003731"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HFO5820537.1"
                     /translation="MQILADLLNTIPAIDPAAMSRAQRHIDGLLKPVGSLGKLEALAI
                     QLAGMPGLNGIPHVGKKAILVMCADHGVWEEGVAISPKEVTAIQAENMTRGTTGVCVL
                     AAQAGANVHVIDVGIDTAEPISGLINMRVARGSGNIASAPAMSRRQAEKLLLDVICYT
                     RELAKNGVTLFGVGELGMANTTPAAAIVSTITGRDPEEVVGIGANLPTDKLANKIDVV
                     RRAITLNQPNPRDGVDVLAKVGGFDLVGIAGVMLGAASCGLPVLLDGFLSYAAALAAC
                     QMSPAIKPYLIPSHLSAEKGARIALSHLGLEPYLNMDMRLGEGSGAALAMPIIEAACA
                     IYNNMGELAASNIVLPGNTTSDLNS"
     gene            complement(5370..6113)
                     /gene="cobS"
                     /locus_tag="ACHKBL_003732"
     CDS             complement(5370..6113)
                     /gene="cobS"
                     /locus_tag="ACHKBL_003732"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0051073 - adenosylcobinamide-GDP
                     ribazoletransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HFO5820538.1"
                     /translation="MSKLFWAMLSFITRLPVPRRWSQGLDFEHYSRGIITFPLIGLLL
                     GAISGLVFMVLQAWCGVPLAALFSVLVLALMTGGFHLDGLADTCDGVFSARSRDRMLE
                     IMRDSRLGTHGGLALIFVVLVKILVLSELTLRGEPILASLAAACAVSRGTAALLMYRH
                     RYAREEGLGNVFIGKIDGRQTCVTLGLAAIFAAVLLPGMHGVAAMVVTMVVIFILGQL
                     LKRTLGGQTGDTLGAAIELGELVFLLALL"
     gene            complement(6110..6655)
                     /gene="cobU"
                     /locus_tag="ACHKBL_003733"
     CDS             complement(6110..6655)
                     /gene="cobU"
                     /locus_tag="ACHKBL_003733"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HFO5820539.1"
                     /translation="MMILVTGGARSGKSRHAEALIGDSSQVLYIATSQILDDEMAARI
                     EHHRQGRPEHWRTVERWQHLDELIHADINPNEAVLLECVTTMVTNLLFDYGGDKDPDE
                     WNYQAMEQAINAEIQSLIAACQRCPAKVVLVTNEVGMGIVPESRLARHFRDIAGRVNQ
                     QLAAAANEVWLVVSGIGVKIK"
     gene            complement(6789..7226)
                     /locus_tag="ACHKBL_003734"
     CDS             complement(6789..7226)
                     /locus_tag="ACHKBL_003734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001524956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HFO5820540.1"
                     /translation="MKTIKPESIALFCLLSGDVDLAKHLAEILPITCFVGEDWQQDFQ
                     PLNGGSVQSLSDAYSDYSVLLLIAAESEISVPEWLPHSEGTTVIVIAEHNARLLTGPA
                     GGAVALIHYLNEICHARETAVNDAFCRTLTRGSLAIPQCHLLS"
     regulatory      complement(7444..7619)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(7708..8622)
                     /gene="pocR"
                     /locus_tag="ACHKBL_003735"
     CDS             complement(7708..8622)
                     /gene="pocR"
                     /locus_tag="ACHKBL_003735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein PocR"
                     /protein_id="HFO5820541.1"
                     /translation="MISAATLNSELINKIAQDFAQATSLAVVVVNIHGDEISELFNFT
                     PFCQMMRQHPQLSTRCRMSDRCGGLEASKSDQPCIYRCHAGLTDFSIPLVIAGHLVGF
                     VLCGQVRLRNDDVDLVDILNVDDCWQADPELLNEFRKVPEMDYSRVMASADLLKLIVE
                     NCLKKQLNFVVIKENKSQSDSVRQTRAPNPHDNKMKKALRYIDAHLSDDLRLEDVASH
                     VYLSPYYFSKLFKKYHGIGFNAWVNQQRMASARELLCHSDWSIASIARNLGFSQTSYF
                     CKVFRQTYQVTPQVYRQQVSENAAMETV"
     gene            complement(8825..9634)
                     /gene="pduF"
                     /locus_tag="ACHKBL_003736"
     CDS             complement(8825..9634)
                     /gene="pduF"
                     /locus_tag="ACHKBL_003736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004016432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol diffusion facilitator PduF"
                     /protein_id="HFO5820542.1"
                     /translation="MNDSLKAQCVAEFLGTGLFLFFGIGCLCALKVTGASLGLWEICI
                     IWGLGISLAVYLTAGISGAHLNPAVTIALWLFASFPARKVLPFCVAQLAGAFGGAVLA
                     YSLYSSLFTDFESAHNIVRGSAESLQLASIFSTYPAAAINVWQAALVEVVITSMLMGL
                     IMALTDDGNGVPKGPLAPLLIGILVAVIGASTGPLTGFAMNPARDFGPKIFTWFAGWG
                     EIAMTGGRNIPYFIVPIIAPIIGACVGAAIYRYLIAKNLPVNSVTPKEISE"
BASE COUNT         2774 a         2616 c         2118 g         2424 t
ORIGIN      
        1 tagcggtatt ttcgtattcc ctgcaccacg aagtgccccg ctaccaataa gagtgatggc
       61 agctgctgga taactgagta cggtcagctc cagataagtc aacgccagtg ctttaacttc
      121 tgtcgtggca tcacccgcga cgaaatcaat aatttgttcg ccaaaatgat gaattagcgt
      181 tgccaacagt acggcaaaca acgtcatgat caccaatgac tgccgcgtcg ccaccctcgc
      241 tcgtcgtcga tctcgcttac cgagactaaa tgctacaacg acagtagtac caagatcgat
      301 agctgcaaaa aaagccataa cgaccatatt gaagctgtcc gccaacccca cgccggccat
      361 cgcatctttt cccagccagc tgaccagaaa agtgctcagg acccccatca acaggacaca
      421 ggcgttctcc atgaagatag gaacagcaag cggggttatc tcgcgccaga acaacacttt
      481 gtagctcttg cgtttagagt gccagcgagt gccgtgaaca acctggcgta aagcagagga
      541 gatattcaaa gccgacctta attgcagaaa gtgaaaccac atttcaaatg atgagggaga
      601 atcagcaaag ctgcaaagat tttcgccaac aaattgtctg caaatgcaac aaactgttga
      661 tagaaacggc aaacagttgg gaaatttaaa aatcgggttt gacaaaatat ttttcgccgt
      721 taagatgtgc ctcaacagcg attcctctgt agttcagtcg gtagaacggc ggactgttaa
      781 tccgtatgtc actggttcga gtccagtcag aggagccaaa tttctgaaaa gcccgctttt
      841 atagcgggct ttttgctata tctgataatc aatttcctct tcactgtttt ccatcacctg
      901 gcgcttgata tcctcaactg acagtcctgc attacaaagt tccagaaagc gccagacata
      961 gttacgctga agttgccctc gcttcagtcc caaccagaca gtattagcat cgaaaagatg
     1021 ccgcgtatcc aggcggatta aattcttttc ctcttgttcg ccgctggatt gttcggcaac
     1081 taatccgatc ccaagcccaa gagcaacata ggttttaatg acatcagaat cctgtgcact
     1141 taatacaata tctgccggca aacctttgcg ggcaaatgcg tcatcaatgc gtgagcgccc
     1201 cgtaattccc tgtcggtaag taattaacgg ccacttcgct attgattcca gcgtcaatgg
     1261 tgtaatttgc gttaagggat gatcgagtgg aacaagcaaa ctatggtgcc agcgaaacca
     1321 cgggaaggcg acgagctgcg ggtcattact caaacgctcg ctggcgatac caatatcagc
     1381 ttcgccattt tgcaacaacg tcgcaatttc ctgtggcgta ccctagatta gctcgagccg
     1441 aacctccggg aaaagttcgc gaaaagcttt aatgacctct ggcaagctat aacgtgcctg
     1501 agtatgcgtc gttgcaatag tgagaacacc agacgtatcg ttggtaaaca ggtctgcaag
     1561 ccgacgaaca ttactggctt cattcagaat acgttctgca atgaccagta atgctttgcc
     1621 cggttcagtc atgcccagca gtcgcttacc tcgtcgaaca aatatttcga tgccaagttc
     1681 atcctccagt tcccgaatat gacggctaac gcctgactgt gaggtaaaaa gcatattcgc
     1741 aacctctgtc aggttgtaat cctgacgtgc agcctcgcgg attatcttta gttgttggaa
     1801 attcacggta aactccgggc agttcagata tcccgttatt gttaaagtct aatgcccggc
     1861 ataacaaata ataaaaaccc gcatcttatt ccatcccgat ataacgctta gctcaccaat
     1921 tgccactgcc ttttttccat cactggagaa ctaatcactg acattaacaa ctctttcact
     1981 gcctgtgcct gtggtgacaa gtttgctctg gcgggtaaat ttaaggacaa agagagactc
     2041 attgaaggag tggtaatgcg tgacatccac ccatttactg cgccacataa cgaacgcgca
     2101 gccgattcag gtaatactgc aacgcccatg ccgctggcaa tcgctgcggt aagcgtggca
     2161 atagactcaa tttcaccaat gacttttgcc gtgagccgcc gtagggaaaa agcctcatca
     2221 acacgaagtc taacagcgct gtaatcactg gggagaaaaa ggttcatttg cgcaatagca
     2281 ttaacatcaa cgctttgccc cgggcaatct tgagttccta ccaggaaaag atcttctttc
     2341 agcaaagcct gactggatac cccagccaca ggggaatgct cataaatcac cgccatatcg
     2401 agttggtgat ttatcagttt ttcgttaagc actgcaccac tattttcatg aagatagata
     2461 acgacctccg ggaattcagc gcgaaccgcc tgtaataagg gcatggtgat ggatgacgca
     2521 gcggttcctg gcgcaaagcc aatcgagact tgccccgata atgactgacc aacgttatgc
     2581 accgccagtt gggcctgctc acactgacgt aaaatggccc gtgcatgggt atagagaatt
     2641 tttccggcgt ctgttggcgt aacgccccgt tttgtgcgaa tcaaaagctg ttgatttaac
     2701 tcaccttcca gtgtggcaac ctgctggctg agcgctggtt gtgcgatatg caatacttca
     2761 gcagcctggg tcaggctacc aatatctaca atttttacga agtatttcag gcgtctgaag
     2821 ttcatgttgc ctccggtttt taagaatcgg cccaagtgcc gccattactt acaaccagaa
     2881 cgcaagatgc ttgccagttc tattttagcg ttgatgcgca agctaaccaa ctgtcaaata
     2941 agagattatg atagattcgt cattggccct ttttatcagc agtcgcattc acctgcccta
     3001 taaaaggagg gtatgcacca cgatggttca ttacccaata agattgaaag ctcaccactt
     3061 tgttgaaatt ggcagcaaac aaacaaaaaa atgcatttca ccctttgaca tcaccatgca
     3121 ctgccattaa tatgcgcccc gttcacacga ttcctctgta gttcagtcgg tagaacggcg
     3181 gactgttaat ccgtatgtca ctggttcgag tccagtcaga ggagccaaat tcaaaaaagc
     3241 ctgcttcgag caggcttttt gctttctaat taccaacgct cttaaaacat ctgtcttgaa
     3301 ccagaactaa tttgcacagg cattcccgat cgatgttgca acgcagcatt tgcgcgattt
     3361 acatcaactt cttgcccgtt gataaacgcc cgcaaagatg gggttaccgg caatggcact
     3421 tttcggtcag actcatattc tgcacgattg cgcgacagcg gctcatgaac ttccagccac
     3481 tttgaaccat ccggttccgt tgtgtatttc actggctgat caataatttg tacacgagtg
     3541 cctacaggaa cattatcaaa cagatatttg atatcgtcat tgcgcagacg aatacagccc
     3601 tgacttaccc ggagcccaat accaaaattg gcattggtac cgtggatggc atacaacctg
     3661 ccaatataaa tcgcgtacag ccccatgggg ttatcgggcc ccgcaggaac aaatgcgggc
     3721 aaactctccc ctcgtttcgc atattcgcgc cgagtgttcg gcgttggcgt ccaggttggt
     3781 gcttcttgtt tacgttcaac ggtagtcacc cagttacgcg gggtttctcg cccagcctgg
     3841 ccgataccaa taggaaagac ttccactgta ttactgtctg gcggatagta ataaagacgc
     3901 atttcagcga cgttaaccac aatcccttta cggacagtgg cgggcaaaat cagttgctgc
     3961 ggaatggtga gttgtgagcc agacttcggc aaaaaaacat cagcgcccgg attcgcttcc
     4021 agcatattac ttaacccttg cccgtattgt gcggcaaaag tctccagcgg ctgagtattg
     4081 tgatcaggaa cagttacagt aagcgactgc cccactaaac ggctaccctc tggaggtaat
     4141 ggataagtta ccgccaggct attttggctg gcaaaaagca gagcaaatga gcaaagaata
     4201 tttacacgac gcatcatgtc cctttcctaa gtcgcgaata ctatccgata agtatagctt
     4261 ttatcagact tttcgttttt agctgttcaa atcagaagtc gtattccccg gtagaacaat
     4321 attactggca gcaagttcgc ccatattgtt gtatatcgca caagcagctt cgatgatggg
     4381 catcgccaga gctgcaccac tcccctcacc taaacgcata tccatattga gataaggctc
     4441 cagccccaaa tgcgacagcg ctatacgcgc gcctttttct gccgacaagt gagaaggaat
     4501 gagatacggt ttgattgcag gagacatctg gcaggctgcg agcgcagcag cataagaaag
     4561 aaatccatcc agcaacacgg gtaaaccgca ggaagcagca cctaacatca ctccagctat
     4621 tccgaccaaa tcaaatccac ccacttttgc caggacatca acaccatccc gaggatttgg
     4681 ttgattcaac gtaatcgccc gacgcacaac atcaatttta ttagccagtt tatctgtcgg
     4741 caggtttgcg ccaatcccaa ccacttcttc aggatcccgg ccagtgattg tgctgactat
     4801 tgccgctgcc ggtgtcgtgt ttgccatccc cagttcacct acaccaaaca gcgtgacacc
     4861 gttttttgcc agctcccgcg tataacatat gacgtccaaa agcaactttt cagcctgacg
     4921 gcgactcatt gccggagctg aagcaatatt gccgctacct cgtgcgacac gcatgttgat
     4981 aagcccggag ataggctcag cagtatcaat accaacatca attacgtgga cgttagcgcc
     5041 cgcttgtgct gccagcacac acacgccggt tgttccgcgg gtcatatttt cagcctgtat
     5101 ggctgtcact tcttttggag aaatagcgac cccttcctcc cagacgccgt gatcggcaca
     5161 cataaccagt atcgcttttt tgcccacatg cggtatgcca ttcaaccccg gcattcctgc
     5221 cagttgtatg gcaagcgcct ccagctttcc caggctacca acaggtttga gtaacccgtc
     5281 aatatgccgt tgtgcacgcg acatagcggc aggatcgatg gctgggatcg tattcagtaa
     5341 atcggcaagt atttgcatgt cacgtctctt tatagcagtg ccagcaggaa aaccagttca
     5401 ccaagctcta tggctgctcc cagcgtatcg cccgtttgcc cgcccagcgt acgtttgaga
     5461 agctgaccga ggatgaaaat caccaccatc gtcaccacca tagcagccac accatgcata
     5521 cccggcaata atacggcagc aaaaatagcg gctaagccga gtgtgacgca ggtttgtcgc
     5581 ccatcaattt tgccaatgaa cacattgcca agcccttctt cccgcgcata acgatgacga
     5641 tacatcaata atgcggcagt cccgcggctg accgcacatg ctgccgccag cgatgcaagg
     5701 atcggttcgc cacgcagagt caactcgctt aacaccagaa tctttaccag taccacaaaa
     5761 atcaatgcca gaccgccgtg ggtgcctaaa cggctatcac gcatgatttc caacatgcga
     5821 tcgcggctac gtgcagaaaa tacgccatca caggtatcag cgaggccgtc cagatggaat
     5881 cccccggtca tcagcgccag cacaagtacg ctaaacagtg ccgccagtgg tacgccacac
     5941 catgcctgca gcaccatgaa gaccagcccg ctaatcgcgc caagcaataa tccaatcaaa
     6001 ggaaaagtaa taataccgcg agaataatgc tcgaaatcca gtccctggga ccagcgacgc
     6061 ggtacaggca gacgcgtaat aaatgagagc atcgcccaaa ataatttact catttgattt
     6121 ttactccaat acccgaaacc accagccata cttcatttgc cgcagcggcc aactgctgat
     6181 ttacccgccc ggcaatatca cgaaaatgtc gtgccagacg actctccggc acaatcccca
     6241 ttcccacttc gttagtcact aatacaacct ttgcggggca acgttggcag gcagcaatca
     6301 acgactgaat ctcagcatta atcgcctgtt ccatcgcctg ataattccat tcatcagggt
     6361 ctttatcgcc gccataatca aacaacagat tagtcaccat tgtggtaacg cattccagca
     6421 acacagcctc attcgggtta atgtctgcat gaattaattc atcaagatgt tgccaacgct
     6481 ccactgtgcg ccagtgctcc gggcggcctt gccgatgatg ttctatccgt gcagccatct
     6541 catcatcaag gatttgcgag gtggcgatat acagaacctg tgaagagtcc ccaataagcg
     6601 cctctgcgtg gcgactcttc ccgctccgtg cgccccccgt caccaaaatc atcataccga
     6661 ctcctgattc aattgtatga tggtattcat tgaacagaac aatggatccc ccaccgttgc
     6721 tgaccgcaaa acatttacgc gattaatact gcgcgtaata taattaaaaa tccagcattc
     6781 tcaatccatt acgaaagaag atggcattgc ggaatagcca gacttcctcg tgttaacgtc
     6841 cgacaaaatg cgtcattgac agcggtttct ctggcatgac agatttcatt cagataatgg
     6901 atcaatgcca cagccccacc agccggacca gtcagcaaac gggcattgtg ttctgcaatc
     6961 acaattaccg tcgttccctc agaatgaggg agccattctg gtacggatat ctcgctttca
     7021 gctgctatca atagcagcac tgaataatcg ctatacgcat ctgataatga ttgcacagac
     7081 ccaccgttaa gaggctggaa atcttgctgc cagtcctcac caacaaaaca ggttattggc
     7141 aatatttcag ccagatgttt cgccaggtcc acatctccag ataagaggca aaaaagagct
     7201 atcgattcgg gttttatcgt tttcatgcct ctcgccctcc acttagtttc ctgtaatgtc
     7261 ggtctggttg atgagccatg aaacaagatt gccacacctt aagtgcagcc ctggtgcctg
     7321 tcggcactgc gatgaaagaa ctaaatgatg ccgccatacc ctgatccttt cagggtggta
     7381 ggggtataaa gacgcgccgg tactctaccg gacgcttccg ggcaacccac ttcccaccga
     7441 agttgttggt attgactgac agactggtct tctggcttag cgtcatcctc ccccgacctt
     7501 cccaatctcg cgatcagtgg tattttcggg tttgtcagca ttacagcagc gggggctgcg
     7561 agggattttc accctcttcc caggcacaaa tgtgccacac ctgtcagcgt atgttatgag
     7621 ctgatttcac ttttcccata acacgtaaat aacgttaatt attcattcca gcgtttattt
     7681 aaaccttccc tttttttagc atttactcta taccgtttcc atcgccgcat tttcactcac
     7741 ttgctgtcga tacacctgcg gcgtaacctg ataagtctga cggaaaactt tgcaaaaata
     7801 actggtttgc gaaaaaccta aattacgcgc aatgctggcg atgctccagt cactatggca
     7861 cagtaactca cgagcgctgg ccatccgttg ttggtttacc cacgcattaa aaccgattcc
     7921 atggtatttc ttgaataact tactgaaata ataaggactt agataaacgt gcgaggcgac
     7981 atcttccaga cgcaaatcgt ctgataaatg cgcatcaata tagcgtaaag cttttttcat
     8041 tttattgtcg tggggattag gcgcacgagt ctggcgcacc gaatctgact gagatttgtt
     8101 ctctttaata accacaaaat tgagttgttt tttgaggcaa ttttccacaa tcaacttcaa
     8161 caaatcagca gaggccataa ctcttgagta atccatctca ggaactttgc ggaattcatt
     8221 aagtaattcg ggatctgcct gccagcaatc atcgacattc agaatatcga ccaaatcgac
     8281 atcatcatta cgcagacgaa cttgcccgca caatacaaaa ccaactaaat gtccggcgat
     8341 taccagtgga atagagaaat cggttaatcc ggcatgacag cgataaatac aaggctgatc
     8401 ggatttagag gcttccagac ccccacaacg atcgctcatg cgacatcgag tgctcagctg
     8461 agggtgttgc cgcatcatct gacaaaatgg agtgaagtta aacagctcgg aaatttcatc
     8521 gccgtgaata ttgaccacga caaccgcaag gctggtcgcc tgcgcaaaat cctgcgcgat
     8581 tttattaatg agttctgaat taagtgtcgc cgcagaaatc atgataaaac ctcccagtta
     8641 atttattgtt atagcaaaac aattgtttta catttaatct tcacatcatc cttgatgatt
     8701 tactgacgag aagcacaaag gacaataaca aaagcctccg tgaagcggga gactttttaa
     8761 taagttcgga aagcagatca caaacagtaa aaaaataaac gtttataact gccagattat
     8821 ttcgttattc gctgatttct ttaggcgtta cactattgac aggtaaattc tttgcaatga
     8881 gatagcgata aatcgccgca ccaacacatg ccccaatgat cggtgcaata atgggtacaa
     8941 tgaaataagg aatattgcgt ccaccagtca tcgcaatttc cccccaaccc gcaaaccagg
     9001 tgaaaatttt aggtccaaaa tcacgcgctg gattcatggc aaaaccggtt aatggtccgg
     9061 tagaagcacc aataacagca accagaatac caatgagtaa aggcgcaagc ggtcctttgg
     9121 gtacgccatt accgtcatcg gtcaacgcca taatcaaccc cattaacatg gacgttatga
     9181 ccacttcgac caacgctgct tgccatacat taatcgccgc tgctggataa gtactgaaga
     9241 tactggcgag ttgtaaactt tctgcgctac cacgcactat attgtgagca gattcaaaat
     9301 cggtaaacaa actgctgtag agagaatatg ccagaaccgc accaccaaag gcacccgcta
     9361 attgcgccac acaaaatggc agcactttac gggctgggaa agaggcaaac agccacagag
     9421 caatggtaac tgccgggttg agatgagcgc cggaaatacc tgccgtaagg taaacagcaa
     9481 gcgaaatccc taatccccaa atgatacaaa tttcccacag tcccagactt gccccggtaa
     9541 ccttcagggc gcacaaacaa ccaatgccaa aaaagaggaa cagtccggtg cccaaaaact
     9601 cggcaacgca ttgcgccttg agtgaatcat tcatagtgac accttcttta cggagttttt
     9661 ctttatcagc caactatgcg ctgattgttg tgtataccga gcgcaactat aaaaaattgc
     9721 tctgcagaaa atattgccaa catgcatgac agactcgtgt aaaaatcgtg ctaacataac
     9781 aaattattgt cattgccgaa tcatgcaaca cagcgttaat cagtgttgaa agtaataatt
     9841 tccaccaaac ttttacaata aaaaattgcc atcatgacat aataaaattg aaacaaatat
     9901 cacagttcct tccttcgaac caaactcaac ga
//
LOCUS       DAZFLD010000171         9808 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.172, whole genome shotgun sequence.
ACCESSION   DAZFLD010000171 DAZFLD010000000
VERSION     DAZFLD010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9808
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.172"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            70..588
                     /gene="yfiR"
                     /locus_tag="ACHKBL_003737"
     CDS             70..588
                     /gene="yfiR"
                     /locus_tag="ACHKBL_003737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HFO5820543.1"
                     /translation="MRFSHRLFLLLILLLTGAPILAQEPSDVAKNVRMMVSGIVSYTR
                     WPALSGPPKLCIFSSSHFSNALQENGTTSLPYLPVIIHTQQEAMISGCNGFYFGNESP
                     TFQMELTEQYPSKALLLIAEQNTECIIGSAFCLIIHNNDVRFAVNLDALSRSGVKVNP
                     DVLMLARKKNDG"
     gene            578..1804
                     /gene="dgcN"
                     /locus_tag="ACHKBL_003738"
     CDS             578..1804
                     /gene="dgcN"
                     /locus_tag="ACHKBL_003738"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcN"
                     /protein_id="HFO5820544.1"
                     /translation="MMDNDNSLNKRPTFKRALRNISMTSIFITMMLIWLLLSVTSVLT
                     LKQYAQKNLALTAATMTYSLEAAVVFADGPAATETLAALGQQGQFSTAEVRDKQQNIL
                     ASWHYTHKEPGDTFSNFISHWLFPAPIIQPIRHNGETIGEVRLTARDSSISHFIWFSL
                     AVLTGCILLASGIAITLTRHLHNGLVEALKNITDVVHDVRSNRNFSRRVSEERIAEFH
                     RFALDFNSLLDEMEEWQLRLQAKNAQLLRTALHDPLTGLANRAAFRSGINTLMNNSDA
                     RKTSALLFLDGDNFKYINDTWGHATGDRVLIEIAKRLAEFGGLRHKAYRLGGDEFAMV
                     LYDVQSESEVQQICSALTQIFNRPFDLHNGHQTTMTLSIGYAMTIEHASAEKLQELAD
                     HNMYQAKHQRAEKLVR"
     gene            1820..2302
                     /gene="yfiB"
                     /locus_tag="ACHKBL_003739"
     CDS             1820..2302
                     /gene="yfiB"
                     /locus_tag="ACHKBL_003739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HFO5820545.1"
                     /translation="MIKHLVAPLIFTSLILTGCQSPQGKFTPEQVAAMQSYGFTESAG
                     DWSLGLSDAILFAKNDYKLLPESQQQIQTMAAKLASTGLTHARMDGHTDNYGEDSYNE
                     ALSLKRANVVADAWAMGGQIPRSNLTTQGLGKKYPIASNKTAQGRAENRRVAVVITTP
                     "
     gene            complement(2379..2726)
                     /gene="rplS"
                     /locus_tag="ACHKBL_003740"
     CDS             complement(2379..2726)
                     /gene="rplS"
                     /locus_tag="ACHKBL_003740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012768745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HFO5820546.1"
                     /translation="MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRE
                     RTGKAARIKERLN"
     gene            complement(2768..3535)
                     /gene="trmD"
                     /locus_tag="ACHKBL_003741"
     CDS             complement(2768..3535)
                     /gene="trmD"
                     /locus_tag="ACHKBL_003741"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HFO5820547.1"
                     /translation="MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTHD
                     RHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQAGVSEL
                     ATNQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVL
                     GHEASATEDSFAEGLLDCPHYTRPEVLEGMEVPPVLLSGNHAEIRRWRLKQSLGRTWL
                     RRPELLENLALTEEQARLLAEFKTEHAQQQHKHDGMA"
     gene            complement(3566..4114)
                     /gene="rimM"
                     /locus_tag="ACHKBL_003742"
     CDS             complement(3566..4114)
                     /gene="rimM"
                     /locus_tag="ACHKBL_003742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043333.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HFO5820548.1"
                     /translation="MSKQLTAQAPVDPIVLGKMGSSYGIRGWLRVFSSTEDAESIFDY
                     QPWFIQKAGQWQQVQLESWKHHNQDMIIKLKGVDDRDAANLLTNCEIVVDSSQLPQLE
                     EGDYYWKDLMGCQVVTTEGYDLGKVVDMMETGSNDVLVIKANLKDAFGIKERLVPFLD
                     GQVIKKVDLTTRSIEVDWDPGF"
     gene            complement(4133..4381)
                     /gene="rpsP"
                     /locus_tag="ACHKBL_003743"
     CDS             complement(4133..4381)
                     /gene="rpsP"
                     /locus_tag="ACHKBL_003743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HFO5820549.1"
                     /translation="MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIAS
                     EKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNKAA"
     gene            complement(4518..5879)
                     /gene="ffh"
                     /locus_tag="ACHKBL_003744"
     CDS             complement(4518..5879)
                     /gene="ffh"
                     /locus_tag="ACHKBL_003744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HFO5820550.1"
                     /translation="MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVA
                     LPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAV
                     VLMAGLQGAGKTTSVGKLGKFLREKHKKKVLVVSADVYRPAAIKQLETLAEQVGVDFF
                     PSDVGQKPVDIVNAALKEAKLKFYDVLLVDTAGRLHVDEAMMDEIKQVHASINPVETL
                     FVVDAMTGQDAANTAKAFNEALPLTGVVLTKVDGDARGGAALSIRHITGKPIKFLGVG
                     EKTEALEPFHPDRIASRILGMGDVLSLIEDIESKVDRAQAEKLASKLKKGDGFDLNDF
                     LEQLRQMKNMGGMASLMGKLPGMGQIPDNVKSQMDDKVLVRMEAIINSMTMKERAKPE
                     IIKGSRKRRIAAGCGMQVQDVNRLLKQFDDMQRMMKKMKKGGMAKMMRSMKGMMPPGF
                     PGR"
     gene            6046..6837
                     /gene="ypjD"
                     /locus_tag="ACHKBL_003745"
     CDS             6046..6837
                     /gene="ypjD"
                     /locus_tag="ACHKBL_003745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HFO5820551.1"
                     /translation="MPVFALLALVAYSVSLALIVPGLLQKNGGWRRMAIISAVIALVC
                     HAIALEARILPDGDSGQNLSLLNVGSLVSLMICTVMTIVASRNRGWLLLPIVYAFALI
                     NLALATFMPNEYITHLEATPGMLVHIGLSLFSYATLIIAALYALQLAWIDYQLKNKKL
                     AFNQEMPPLMSIERKMFHITQIGVVLLTLTLCTGLFYMHNLFSMENIDKAVLSIVAWF
                     VYIVLLWGHYHEGWRGRRVVWFNVAGAVILTLAYFGSRIVQQLIS"
     gene            6858..8144
                     /gene="yfjD"
                     /locus_tag="ACHKBL_003746"
     CDS             6858..8144
                     /gene="yfjD"
                     /locus_tag="ACHKBL_003746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026171.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HFO5820552.1"
                     /translation="MEHISTTTLIIILIIMVVISAYFSGSETGMMTLNRYRLRHMAKQ
                     GNRSAKRVEKLLRKPDRLISLVLIGNNLVNILASALGTIVGMRLYGDAGVAIATGVLT
                     FVVLVFAEVLPKTIAALYPEKVAYPSSFLLAPLQILMMPLVWLLNAITRMLMRMMGIK
                     TDIVVSGSLSKEELRTIVHESRSQISRRNQDMLLSVLDLEKMTVDDIMVPRSEIIGID
                     INDDWKSILRQLSHSPHGRIVLYRDSLDDAISMLRVREAWRLMSEKKEFTKETMLRAA
                     DEIYFVPEGTPLSTQLVKFQRNKKKVGLVVNEYGDIQGLVTVEDILEEIVGDFTTSMS
                     PTLAEEVTPQNDGSVIIDGTANVREINKAFNWHLPEDDARTVNGVILEALEEIPVAGT
                     RVRIGEYDIDILDVQDNMIKQVKVFPVKPLRESVAE"
     gene            complement(8198..8791)
                     /gene="grpE"
                     /locus_tag="ACHKBL_003747"
     CDS             complement(8198..8791)
                     /gene="grpE"
                     /locus_tag="ACHKBL_003747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HFO5820553.1"
                     /translation="MSSKEQKTPEGQAPEEIIMDQHEEIEAVEPEASAEQVDPRDEKI
                     ANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSL
                     DRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMV
                     ESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKAKA"
     gene            8914..9792
                     /gene="nadK"
                     /locus_tag="ACHKBL_003748"
     CDS             8914..9792
                     /gene="nadK"
                     /locus_tag="ACHKBL_003748"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HFO5820554.1"
                     /translation="MNNHFKCIGIVGHPRHPTALTTHEMLYRWLCTKGYEVIVEQQIA
                     HELQLKNVKTGTLAEIGQLADLAVVVGGDGNMLGAARTLARYDIKVIGINRGNLGFLT
                     DLDPDNAQQQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVVLHPGKVAHMI
                     EFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSA
                     RPLVINSSSTIRLRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYS
                     YFNTLSTKLGWSKKLF"
BASE COUNT         2364 a         2620 c         2318 g         2506 t
ORIGIN      
        1 aatgatattt taatgagcat tctgaaaaaa tatcgatgca tttcgagcga agatggtgag
       61 gatccctgaa tgcgtttttc tcaccgactg ttcttactgc tgattctcct gctgacggga
      121 gcaccaatac tcgctcagga accttctgac gttgcgaaaa acgtgcgtat gatggtttcc
      181 ggtattgtca gttacacccg ctggcctgcg ctatcagggc caccaaagtt atgtatcttt
      241 tcatcttcac attttagtaa cgccctacaa gagaatggca cgacatcttt gccttatcta
      301 cctgtcatca ttcacactca acaagaggcg atgatttcag gctgcaatgg tttttatttt
      361 ggcaacgagt caccgacatt ccagatggaa ttaaccgaac aatatccgtc aaaggcgtta
      421 ttattaattg ccgaacaaaa taccgagtgc attattggta gcgccttttg tctaatcatc
      481 cacaataatg acgtcagatt tgccgtaaac ctggatgcct tatcgcgtag cggtgtaaag
      541 gtcaacccgg atgtcttaat gctcgcacgg aagaaaaatg atggataacg ataattctct
      601 taataagcgc cccacgttta aaagagcatt acgcaacatc agtatgacca gcatatttat
      661 cactatgatg ctgatctggt tgctgctttc cgtgacctcg gtgctgaccc tgaaacagta
      721 cgcgcaaaaa aacctggcac tgacagcagc aacaatgact tacagtctgg aagcagctgt
      781 cgtttttgcc gatggccctg cagcaactga aacactggca gcgctgggcc agcaaggcca
      841 attttcaact gcagaagtgc gtgataagca gcaaaatatt ctggcatcct ggcattacac
      901 ccataaggaa ccaggcgata ctttcagcaa tttcataagc cactggctct tccctgcccc
      961 catcattcag ccgattcgtc acaatggtga aaccattggc gaagtacgct taactgctcg
     1021 cgacagttca atcagccatt ttatctggtt ttcgctcgct gtcctgaccg gttgtattct
     1081 gctggcatca ggcatcgcaa ttaccctcac ccgccatttg cacaatggcc tggtggaagc
     1141 gctgaaaaat atcaccgatg tcgtacatga tgtgcgttct aaccgcaatt tttcccgacg
     1201 agtttcggaa gaacgtatcg ctgagtttca ccgcttcgct ctcgacttca acagtctgct
     1261 ggatgaaatg gaagagtggc agcttcgttt acaggccaaa aatgcgcagc ttttacgtac
     1321 cgcgctacat gacccattaa ccgggctggc taaccgcgca gcgtttcgta gcggcatcaa
     1381 cacgttgatg aacaattccg atgcccgaaa aacgtcggcg ttactatttc ttgatggcga
     1441 taatttcaaa tatatcaatg atacctgggg tcatgcgacg ggcgatagag tcttgattga
     1501 aatcgcaaaa cggttagctg aatttggcgg actgcgacat aaagcatacc gcctgggcgg
     1561 cgatgaattc gcgatggtgc tctatgatgt acagtcggaa tctgaagtgc agcagatatg
     1621 ctcagcactg acacaaatct ttaatcgccc gtttgatctt cataatggtc atcagaccac
     1681 catgacatta agcattggtt acgcgatgac cattgagcat gcctctgcgg aaaaattaca
     1741 agagctggcc gatcacaata tgtatcaggc caaacaccag cgtgccgaaa agctggtgag
     1801 ataacaagga tatatcgata tgataaagca cctggtagca cccctgattt tcacctcact
     1861 aatactgact ggctgccagt cccctcaggg aaagtttact cctgagcaag tcgccgctat
     1921 gcaatcttat ggatttactg aatccgccgg cgactggtcg ctgggcttat cagatgccat
     1981 tctgttcgca aaaaatgact acaaattgct cccggaaagc cagcaacaga tccaaaccat
     2041 ggcagccaaa ttggcctcga cagggctaac gcatgcccgt atggatggac acaccgacaa
     2101 ctatggtgaa gacagttaca acgaagcctt atcattgaaa cgggcgaatg tcgtggccga
     2161 tgcatgggct atgggtggac aaattccgcg cagcaatctc accacacagg gtttaggaaa
     2221 aaaatatccc atagccagta acaagaccgc ccagggccgc gccgagaacc gccgcgtcgc
     2281 agtggtgatt actacccctt aaaacacgag caaacagata aaaaaagggc cagccaattg
     2341 gccagccctt cttaacagga tgtcgcttaa gcgaaatctt agttaagacg ctctttgata
     2401 cgagcagcct taccagtacg ctcacgcagg tagtacagtt tagctttacg aacagcacca
     2461 cgacgtttga cagaaatgct gtcaactacc ggagagtgag tctggaagac acgctcaacg
     2521 ccttcgccgt tggaaatttt acgaacagtg aatgcagagt gcagaccgcg gttacgaata
     2581 gcgataacca cgccctcgaa tgcctgcaga cgttttttgg aaccttcaac aacccatact
     2641 ttcacttcca cggtatcacc cggacggaag gaaggtacgt cctgcttcat ctgctcttgt
     2701 tcaagttgct taataatgtt gctcataatt taatctctta tcctgggtaa actgatatct
     2761 cgggggctta cgccatccca tcatgtttat gttgctgttg tgcgtgttcc gttttgaact
     2821 ccgccagcaa ccttgcttgc tcttcagtca gagccaggtt ttccagaagt tcaggtcttc
     2881 taagccaggt acggcccagc gactgtttca aacgccagcg acgtatctcg gcatggttgc
     2941 ccgacagtaa cactggcgga acttccatcc cttctaacac ctcaggccgc gtatagtgcg
     3001 ggcaatccag caatccttca gcaaaggaat cttccgttgc cgaggcttca tgtcccagta
     3061 ctcccggaat aaaccgggaa acggagtcaa tcagcgtcat tgctggtaac tcaccaccac
     3121 tgagaacgta atcgccgatt gaccattctt cgtcaatttc ggtttggatc acgcgctcat
     3181 ctataccttc gtagcgaccg cacaccagaa tcaatttttg attcgttgcc agttcgctga
     3241 cgcccgcttg atcaagcttg cgtccctgtg gtgacagata aatcaccttt gcgccttcac
     3301 ccgccgcggc ttttgctgca tgaatggcgt cccgcaaggg ttgcaccatc attaacatcc
     3361 ccggtccgcc gccgtaagga cgatcgtcca cggtacggtg ccggtcatgc gtgaagtcgc
     3421 gaggactcca gctctggatg ctcagcaggc catttttaac tgcccggcca gttaccccgt
     3481 aatcggtaat tgcgcggaac atttcaggaa acaggctaat tatgccaatc cacatagcgc
     3541 cgtcttttac cgtttatccg gtggtttaaa aaccaggatc ccaatctact tcgattgaac
     3601 gagtagtgag atcgactttc ttgataacct gcccatcgag gaacggtacg agacgttcct
     3661 tgataccaaa cgcatctttc aggtttgcct taatgacgag aacgtcatta gatccggttt
     3721 ccatcatatc gacgacttta ccgagatcgt agccttcagt ggttactacc tggcagccca
     3781 tcaagtcttt ccagtagtag tcaccctctt caagctgagg caactgcgat gaatccacga
     3841 caatttcaca attcgtcagc aggttcgccg catcacgatc gtcaacgcct ttcagcttga
     3901 tgatcatgtc ctgattgtgg tgcttccagc tttccagctg gacttgctgc cactgacccg
     3961 ccttctggat aaaccagggc tgatagtcaa aaatgctttc ggcgtcttcg gtggaagaaa
     4021 acactctgag ccacccacga ataccgtaag acgaacccat ttttcccaaa acgatgggat
     4081 caacaggtgc ttgcgcggtg agttgtttgc tcatcatgac caccgtgaca gattaagctg
     4141 ctttgtttac ttctttgatc agcgcagcaa cgcgatcaga aatagttgcg ccctggccaa
     4201 cccagtgagc gatgcgatcc agatccaggc gagtgccttc ttctttttcg ctagcgattg
     4261 ggttgaagaa accaacgcgc tcgatgaagc gaccgttgcg tgcattacgg ctgtcagcga
     4321 caacaacctg gtagaacgga cgctttttag cgccgtgacg tgctaaacga atagttacca
     4381 taacatcctc ttgtgtgaat aaaacaaccg gaccccatcg aggaacggag tccggtgtca
     4441 tattaaaagc ccgaaaattt tactgatttc tggagaaaat gcaatcagca gttaataact
     4501 ctgtcgcata aggtcattta gcgaccagga aatcccggcg gcatcatacc cttcatgctt
     4561 ctcatcatct tcgccattcc gcccttcttc attttcttca tcatgcgctg catgtcgtcg
     4621 aactgtttca gaagacggtt aacgtcctgc acctgcatac cgcaaccggc agcgatacgg
     4681 cgtttacgcg aacctttgat gatttctggc ttagcgcgct ctttcatcgt catcgagttg
     4741 atgatggctt ccatacgcac cagcacttta tcgtccatct gcgacttgac gttatccggg
     4801 atctgcccca tgcccggcag tttgcccatc aggctggcca tgccgcccat atttttcatc
     4861 tggcgcagtt gctcaaggaa gtcattgaga tcgaagccgt cgcctttttt cagcttgctg
     4921 gctaatttct cagcttgcgc gcggtcaact ttgctttcga tatcttcgat cagcgacagt
     4981 acgtcgccca tgccgagaat acgggaagcg atgcggtccg gatggaacgg ctccagcgcc
     5041 tcggttttct cgccaacacc gaggaacttg atcggtttgc ccgtaatgtg acgaatagag
     5101 agcgccgcac cgccacgagc atcgccgtcg actttagtca gtaccacacc ggtcagcggt
     5161 aacgcttcat tgaatgcttt tgccgtattg gccgcatcct gaccagtcat ggcatcaacc
     5221 acaaacaggg tttcaaccgg attaatcgac gcatggactt gtttgatctc gtccatcatc
     5281 gcttcgtcaa cgtgcagacg accggctgta tccaccagca gcacgtcgta gaatttcagt
     5341 ttggcttctt tcagcgccgc gttaacgata tctaccggct tctgaccaac atcagaaggg
     5401 aagaaatcaa cgcccacctg ctctgccagc gtctcaagct gtttgattgc cgccggacga
     5461 taaacgtcgg cagaaaccac cagcactttc ttcttgtgct tctcgcgcag gaacttaccg
     5521 agtttaccaa cgctggttgt tttaccggca ccttgcaggc ccgccatcag cacgaccgca
     5581 ggcggttgcg cagccaggtt cagggtctgg ttctcttcgc ccatcgccgc aaccagttcg
     5641 ttacggacaa ttttgacgaa ctcctgcccc ggcgtcaggc tcttattaac ttcatgacca
     5701 accgctttct cttttacgcg attgataaac tcacgcacta ccggcagagc gacgtccgcc
     5761 tccagcagcg ccatgcgcac ttcgcgcagc gtatctttta cgttgtcttc agtgaggcgt
     5821 ccacggccac tgatattgcg cagcgtgcgc gacaaacgat cggttaaatt atcaaacatt
     5881 gtctctcgcc tggggtggaa acggttggcc gcaatcgcga cacatcatca gtattttgcc
     5941 gcagtataac atgaaggcgt ctttgttgtt atgcaacggt tggagcagcg ttcacctgac
     6001 gctatactgc ttctctttct tattgctcaa actgtcgaca ttactatgcc cgtttttgct
     6061 ctgctcgcgc ttgtcgccta ctccgtcagt cttgcgctga ttgttcccgg tctgctgcaa
     6121 aaaaacggcg gctggcggcg catggctatt atttctgcgg tcattgcgct ggtctgccac
     6181 gcaatcgctc tggaagcccg catcctgccc gacggtgata gcggacaaaa cctcagcctg
     6241 ctgaacgttg gttcattggt cagtttgatg atctgtacgg taatgaccat tgtggcttct
     6301 cgcaatcgtg gctggttgct gctacccatt gtctatgcct ttgcgcttat caacctggcg
     6361 ctggcgacct tcatgcccaa tgaatacatc acccatctgg aagcaacgcc tgggatgctg
     6421 gtgcacattg gcttatcgct cttttcctat gccacgctga ttatcgccgc cctgtacgcg
     6481 ctgcaactgg cgtggattga ttaccaactg aagaacaaga agctggcgtt taaccaggaa
     6541 atgccgccat tgatgagtat cgagcgtaaa atgttccaca tcacgcagat tggcgtggtg
     6601 ctgctaacgc tcacgctttg cactggcctg ttctacatgc acaacttatt tagcatggaa
     6661 aatatcgaca aggccgtgct ctctatcgtg gcgtggtttg tctatattgt gctgctgtgg
     6721 ggacattatc atgaaggatg gcgtggacgc cgcgtcgtct ggtttaacgt tgcgggtgca
     6781 gtcattctga cactggccta cttcggcagc cgaattgtcc agcagttaat cagctaaacc
     6841 cagaaaagga gtttcccctg gaacacattt ctactactac gttgatcatt attctgatca
     6901 tcatggtggt catttcagcc tatttttccg ggtccgaaac cggaatgatg accctcaacc
     6961 gctatcgtct gcgacatatg gcgaaacagg gtaatcgctc ggccaaacgc gtcgaaaaat
     7021 tgctgcgtaa gccagaccgc ctgattagcc tggtgttaat cggcaataac ctggtcaata
     7081 ttcttgcctc cgcgctaggc actattgttg ggatgcgttt gtacggcgat gcgggcgtgg
     7141 caattgcgac tggtgtgctg acttttgtcg tgctggtttt tgctgaggta ttgccgaaaa
     7201 ccattgccgc gctgtacccg gaaaaagtcg cttatccgag tagttttctg ctggctccgc
     7261 tgcaaatttt gatgatgccg ctggtctggt tgctgaatgc tatcacccgt atgctgatgc
     7321 gcatgatggg tatcaaaacc gatatcgtgg ttagcggctc tttgagcaaa gaagagttgc
     7381 gcactatcgt gcacgaatcg cgctcacaaa tttcccgtcg caatcaggat atgctgctgt
     7441 cggtgctcga tctggaaaaa atgaccgttg atgacatcat ggtgccgcgc agtgaaatta
     7501 tcggtattga tatcaacgat gactggaaat cgattctgcg ccaactctcc cactcacctc
     7561 acgggcgcat cgtgctctac cgtgattcgc tggacgacgc catcagtatg ctgcgtgtgc
     7621 gtgaagcctg gcgactcatg tcggagaaaa aagagttcac caaagaaacc atgctacgcg
     7681 ccgcggacga gatctatttt gtcccggaag gtacgccgct cagcacgcag ttggtgaagt
     7741 ttcagcgcaa caaaaagaaa gtcggcctgg tcgtcaacga gtatggagac attcaggggc
     7801 tggtgacggt tgaagatatt ctggaagaga ttgtcggcga tttcactacc tcgatgtcgc
     7861 caacacttgc cgaagaggtc acgccgcaaa acgacggttc ggtgattatc gatggcaccg
     7921 ccaacgtgcg agaaatcaac aaagccttta actggcatct acctgaagac gatgcccgca
     7981 cggttaatgg cgtcattctg gaagcgctgg aagagatccc tgtcgctggc acccgcgtgc
     8041 gtattggcga gtacgatatc gatattctcg acgtacagga taatatgatt aagcaggtaa
     8101 aagtttttcc tgtgaaaccg ctgcgcgaga gtgtggcgga gtaaaaaaac ggcccggcat
     8161 tcgcatgctg ggccgtgatt tattacgaaa gcagaaatta agcttttgcc ttcgctacag
     8221 tcaccatcgc cgcacgaatc gtacgaccgt tcagcgtata acctttctgc ataatgccca
     8281 gtacgttacc tggcgcaacg tcatcagatt ccaccattgc gatggcctga tgcacatttg
     8341 ggtccagtgg gacgttggtt tcggcgatca cttcaacgcc aaacttacgc acaacatcca
     8401 gcatcgactt cagcgtcagc tcaatacctt caaccatcgc ggacatatcc gggttagctt
     8461 tatcagccac ttccagcgca cgatccaggc tatcaatcac cggcagcaat tcgttgatga
     8521 atttctccag cgcgaatttg tgagcttttt caatatccag ttcagtacga cgacgcaggt
     8581 tttccatttc ggcttttacg cgcaaaatgc cgtcacgttc acgggtctgg gcttcagcca
     8641 gctgagcttc gagattcgca attttttcat cgcgcggatc cacctgctca gcagaagctt
     8701 ctggctcaac tgcctcaatc tcttcgtgct gatccatgat aatttcttcc ggggcttgcc
     8761 cctcaggcgt tttctgttct ttactactca tgaatttctc cgcgtttttt tcgcattcat
     8821 ctcgctaact tcgcttatta tggggatcag tttcagggtt tcaagggaag cactcacatt
     8881 gtcatcaatc ttcgcaacaa ggacctcgga aaaatgaata atcatttcaa gtgtattggc
     8941 attgtgggac acccacggca ccccactgca ctgacaacac atgaaatgct ctaccgctgg
     9001 ctgtgcacaa aaggttacga ggtcatcgtt gagcaacaaa tcgctcacga actgcaactg
     9061 aagaatgtga aaactggcac gctcgcggag attgggcaac tggcagatct cgcggtagtc
     9121 gttggtggcg acggtaatat gctgggcgcg gcgcgcacac tcgcccgtta cgatattaaa
     9181 gttattggaa tcaaccgtgg caacctgggt ttcctgactg accttgaccc cgataacgcc
     9241 cagcaacaat tagccgatgt gctggaaggc cactacatca gcgagaaacg ttttttgctg
     9301 gaagcgcaag tttgtcagca agattgccag aaacgcatca gcaccgcgat taacgaagtg
     9361 gtacttcatc ctggcaaagt ggcgcatatg attgagttcg aagtgtatat cgacgagatc
     9421 tttgcgtttt cgcagcgttc cgatggactg attatttcga cgccaacagg ctccaccgca
     9481 tattccctct ctgcgggcgg tcctattctg accccctctc tggatgcgat taccctggtg
     9541 cccatgttcc cgcatacgtt gtcagcacga ccactggtga taaacagcag cagcacgatc
     9601 cgtctgcgtt tttcgcatcg ccgtaacgac ctggaaatca gttgcgacag ccagatagca
     9661 ctgccgattc aggaaggtga agatgtcctg attcgtcgct gtgattacca tctgaatctg
     9721 attcatccga aagattacag ttatttcaat acattaagca ccaagctcgg ctggtcaaaa
     9781 aaattattct aattttacgc cagcctct
//
LOCUS       DAZFLD010000172         9808 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.173, whole genome shotgun sequence.
ACCESSION   DAZFLD010000172 DAZFLD010000000
VERSION     DAZFLD010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9808
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.173"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(46..1206)
                     /locus_tag="ACHKBL_003749"
     CDS             complement(46..1206)
                     /locus_tag="ACHKBL_003749"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HFO5820555.1"
                     /translation="MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLTLAQ
                     VEKLEEVTAELHQMCLKVVEKVIASDELMTKFRIPKHTWSFVRQSWLTHQPSLYSRLD
                     LAWDGTGEPKLLENNADTPTSLYEAAFFQWIWLEDQLNAGNLPEGSDQFNSLQEKLID
                     RFVELREQYGFQLLHLTCCRDTVEDRGTIQYLQDCATEAEIATEFLYIDDIGLGEKGQ
                     FTDLQDQVISNLFKLYPWEFMLREMFSTKLEDAGVRWLEPAWKSIISNKALLPLLWEM
                     FPNHPNLLPAYFAEDDHPQMEKYVVKPIFSREGANVSIIENGKTIEAAEGPYGEEGMI
                     VQQFHPLPKFGDSYMLIGSWLVNDQPAGIGIREDRALITQDMSRFYPHIFVE"
     gene            complement(1212..1883)
                     /locus_tag="ACHKBL_003750"
     CDS             complement(1212..1883)
                     /locus_tag="ACHKBL_003750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417509.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 family protein"
                     /protein_id="HFO5820556.1"
                     /translation="MKRTKSIRHASFRKNWSARHLTPVALAVATVFMLAGCEKSDETV
                     SLYQNADDCSAANPGKSAECTTAYNNALKEAERTAPKYATREDCIAEFGEGQCQQAPA
                     QAGMAPENQAQAQQSSGSFWMPLMAGYMMGRLMGGGAGFAQQPLFSSKNPASPAYGKY
                     TDATGKNYGAAQPGRTMTVPKTAMAPKPATTTTVTRGGFGESVAKQSTMQRSATGTSS
                     RSMGG"
     gene            complement(2031..3512)
                     /gene="tolC"
                     /locus_tag="ACHKBL_003751"
     CDS             complement(2031..3512)
                     /gene="tolC"
                     /locus_tag="ACHKBL_003751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane channel protein TolC"
                     /protein_id="HFO5820557.1"
                     /translation="MKKLLPILIGLSLSGFSSLSQAENLMQVYQQARLSNPELRKSAA
                     DRDAAFEKINEARSPLLPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSK
                     WRALTLQEKAAGIQDVTYQTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLD
                     QTTQRFNVGLVAITDVQNARAQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAAL
                     NVENFKTDKPQPVNTLLKEAEKRNLSLLQARLSQDLAREQIRQAQDGHLPTLDLTAST
                     GISDTSYSGSKTRGAAGTQYDDSNMGQNKVGLSFSLPIYQGGMVNSQVKQAQYNFVGA
                     SEQLESAHRSVVQTVRSSFNNINASISSINAYKQAVVSAQSSLDAMEAGYSVGTRTIV
                     DVLDATTTLYNAKQELANARYNYLINQLNIKSALGTLNEQDLLALNNALSKPVSTNPE
                     NVAPQTPEQNAIADGYAPDSPAPVVQQTSARTTTSNGHNPFRN"
     gene            3717..4346
                     /gene="nudF"
                     /locus_tag="ACHKBL_003752"
     CDS             3717..4346
                     /gene="nudF"
                     /locus_tag="ACHKBL_003752"
                     /EC_number="3.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose diphosphatase"
                     /protein_id="HFO5820558.1"
                     /translation="MLKPDNLPVTFGKNDVEIIARETLYRGFFSLDLYRFRHRLFNGQ
                     MSHEVRREIFERGHAAVLLPFDPVRDEVVLIEQIRIAAYDTSETPWLLEMVAGMIEEG
                     ESVEDVARREAIEEAGLIVKRTKPVLSFLASPGGTSERSSIMVGEVDATTASGIHGLA
                     DENEDIRVHVVSREQAYQWVEEGKIDNAASVIALQWLQLHHQALKNEWA"
     gene            4347..4769
                     /locus_tag="ACHKBL_003753"
     CDS             4347..4769
                     /locus_tag="ACHKBL_003753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 family protein"
                     /protein_id="HFO5820559.1"
                     /translation="MKRYTPDFPEMMRLCEMNFSQLRRLLPRNDAPGETVSYQVANAQ
                     YRLTIVESTRYTTLVTIEQTAPAISYWSLPSMTVRLYHDAMVAEVCSSQQIFRFKARY
                     DYPNKKLHQRDEKHQINQFLADWLRYCLAHGAMAIPVY"
     gene            4794..5621
                     /gene="cpdA"
                     /locus_tag="ACHKBL_003754"
     CDS             4794..5621
                     /gene="cpdA"
                     /locus_tag="ACHKBL_003754"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004115 - 3',5'-cyclic-AMP
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HFO5820560.1"
                     /translation="MESLLTLPLAGEARVRILQITDTHLFAQKHEALLGVNTWESYQA
                     VLEAIRPHQHEFDLIVATGDLAQDQSSAAYQHFAEGIASFRAPCVWLPGNHDFQPAMY
                     SALQDAGISPAKRVFIGEQWQILLLDSQVFGVPHGELSEFQLEWLERKLADAPERHTL
                     LLLHHHPLPAGCSWLDQHSLRNAGELDTVLAKFPHVKYLLCGHIHQELDLDWNGRRLL
                     ATPSTCVQFKPHCSNFTLDTIAPGWRTLELHADGTLTTEVHRLADTRFQPDTASEGY"
     gene            5621..6202
                     /gene="yqiA"
                     /locus_tag="ACHKBL_003755"
     CDS             5621..6202
                     /gene="yqiA"
                     /locus_tag="ACHKBL_003755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase YqiA"
                     /protein_id="HFO5820561.1"
                     /translation="MSTLLYLHGFNSSPRSAKASLLKNWLAEHHPDVEMIIPQLPPYP
                     SDAAELLESIVLEHGGDSLGIVGSSLGGYYATWLSQCFMLPAVVVNPAVRPFELLTDY
                     LGQNENPYTGQQYVLESRHIYDLKVMQIDPLEAPDLIWLLQQTGDEVLDYRQAVAYYA
                     SCRQTVIEGGNHAFTGFEDYFNPIVDFLGLHHL"
     gene            6231..8123
                     /gene="parE"
                     /locus_tag="ACHKBL_003756"
     CDS             6231..8123
                     /gene="parE"
                     /locus_tag="ACHKBL_003756"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HFO5820562.1"
                     /translation="MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSV
                     DEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSN
                     KNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQVYNIAFENGEKVQDLQVVGTCGKRN
                     TGTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDG
                     LNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQG
                     GTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTK
                     ERLSSRQCAAFVSGVVKDAFTLWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLT
                     SGPALPGKLADCTAQDLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEV
                     SSDEVLASQEVHDISVAIGIDPDSDDLSQLRYGKICILADADSDGLHIATLLCALFVK
                     HFRALVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQRFKGL
                     GEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGD
                     MAEIEV"
     gene            complement(8168..9037)
                     /locus_tag="ACHKBL_003757"
     CDS             complement(8168..9037)
                     /locus_tag="ACHKBL_003757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001060172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2608 domain-containing protein"
                     /protein_id="HFO5820563.1"
                     /translation="MNNKFKIILWMMLSALPALSYAQVKNIVTGEYSAVSKIITEKET
                     LYPSDKILLVFDIDNTLLTSGTRIGGDIWYQWQTDKLPLKPDDSQKVPCLYENTISML
                     YELSPMQLTEPQLPTMLKQWQQKHTAFALTSRAPDTLFPTLRELKRNGIDFSSSALRK
                     KEDTLPPFEKGKLKRSWLYSQGVFFSSGQDKGVILDYMLDKMGNKYDAIVFIDDGQAN
                     IHAMTKMFSKDKWSSTDFTIIHYTRVEDELIKQQGAVITTAQAEKMATDWKTLAGSLA
                     TLFPDRNKLCPIK"
     gene            complement(9051..>9808)
                     /locus_tag="ACHKBL_003758"
     CDS             complement(9051..>9808)
                     /locus_tag="ACHKBL_003758"
                     /inference="COORDINATES: protein motif:HMM:NF017942.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="oligogalacturonate-specific porin KdgM family
                     protein"
                     /protein_id="HFO5820564.1"
                     /translation="FILKGNIMNSYLKISLAGITMLSCLSPTLVCAEQPHGFIDYRHE
                     YLDDTRTHADRVEFGTFFSNGIGLMGELRYNTDEGDKDKWDPSQFGNNGTGLSVVYRF
                     KPLDDKKFWLEPMFWLDTTQYWSTYEYGLSAGYDFSKEWKVSGRFRYDMDKATDKSKG
                     YGNDDRNNRRYDVWIDYRPQKTNFQYQLNLVYYNNGYLTWNDGHKNYTASFKVGYKMG
                     SWIPYMSIADYKGVDKTSSNRQIRWRWGLTYTF"
BASE COUNT         2346 a         2383 c         2552 g         2527 t
ORIGIN      
        1 gattataaga gattgccccg cttatcgggg caatattaag ctgtattact caacaaaaat
       61 atgcggataa aaccgagaca tatcctgggt gatcaatgca cggtcttcac gaatgccaat
      121 tccggcaggt tgatcgttca ccagccagct accaatcagc atatagctgt cgccgaattt
      181 cggtaacggg tggaattgct gaacaatcat cccttcttcg ccatacggac cttccgctgc
      241 ttcaatggtt ttgccgttct caatgatcga cacgtttgcg ccttcacggg agaagatcgg
      301 tttaaccaca tatttttcca tttgcggatg atcatcttcc gcaaaataag caggcagcag
      361 gttcgggtga ttcgggaaca tctcccacag tagcggcaga agcgctttgt tggagataat
      421 gctcttccac gccggttcca gccagcgtac gcctgcatcc tccagcttgg tggagaacat
      481 ctcgcgcagc ataaattccc acggatacag cttgaacagg ttggaaatta cctgatcctg
      541 taaatccgtg aactgacctt tttcgcctaa accgatatcg tcgatgtaga ggaattcggt
      601 agcgatttca gcttccgtcg cgcagtcctg caaatactga atggttccgc gatcttccac
      661 cgtgtcgcga cagcaggtga gatgcagcaa ctggaagcca tactgttcac gcaactcgac
      721 aaagcgatcg attagctttt cttgcagact gttaaactgg tcgctgccct ccggcaagtt
      781 accggcgtta agctgatctt ccagccagat ccactgaaag aacgccgcct cgtatagtga
      841 cgttggtgta tcggcgttat tttccagcag tttaggttcg ccagtgccat cccacgccag
      901 atcaagacga gaataaagcg atggctggtg agtcagccat gactgacgca caaaactcca
      961 ggtgtgtttt ggaatgcgga atttggtcat cagctcatcg ctggcgatca ctttttccac
     1021 cactttcagg cacatctggt gcagttcggc ggtgacttct tccagctttt caacctgggc
     1081 gagggtcaac ttgtagtaag catcttcaca ccagtacggc tcgccgtaca tggtgtgaaa
     1141 attgaaaccg tattcgtggg ctttctcacg ccagtccggg cgctcggtaa tactgactct
     1201 ttccatcggt atcagccacc cattgaacga gaagaggtgc cggttgcgct acgctgcata
     1261 gtgctttgtt tggcaacaga ttcaccaaaa ccgccacggg taacggtagt ggtggtcgcc
     1321 ggttttggtg ccatggctgt cttcggtacg gtcatggtgc ggcctggctg ggctgcgcca
     1381 tagtttttac ccgtcgcgtc ggtatattta ccgtaagccg ggctggctgg gtttttcgag
     1441 gagaacagcg gctgctgtgc aaatcccgca ccgccgccca tcagacgacc catcatgtaa
     1501 ccggccatca gcggcatcca gaaactcccg ctggattgct gggcctgcgc ctggttttcc
     1561 ggtgccatgc cagcctgggc tggtgcctgc tggcactgac cttcgccaaa ttcagcaata
     1621 cagtcttcac gagtggcgta tttcggcgca gtacgttcgg cttctttcag cgcattgttg
     1681 tacgcggtgg tacattcggc gcttttgcct gggtttgcag ctgaacagtc gtcagcattt
     1741 tgatagagag acactgtttc atcactcttt tcacagccag ccagcataaa aacagtggca
     1801 accgcgagag cgactggtgt cagatggcgt gcgctccagt ttttgcggaa cgatgcgtgg
     1861 cgtatggatt ttgtccgttt catttttgtc ttccgggacc agtggtaaat acccatcaga
     1921 atagaggatg gctggtcgaa attgaagcga gaagggggaa gaatgcggca gataacccgt
     1981 atctttacgt tgccttacgt tcagacgggg ccgaagcccc gtcgtcgtca tcagttacgg
     2041 aaagggttat gaccgttact ggtggtagtg cgtgcggatg tttgctgaac gacgggtgcc
     2101 gggctatcag gcgcataacc atcagcaata gcattctgtt ccggcgtttg cggggcaacg
     2161 ttttccggat tagtggaaac cggtttgctc agcgcattgt tcagtgccag aagatcctgc
     2221 tcgttcaacg tacccagagc tgatttaata ttcaactggt taatcaggta gttataacgc
     2281 gcattcgcca gctcttgctt ggcgttgtac agcgtggtgg tcgcatccaa cacatcaaca
     2341 atggtacgcg taccgaccga gtaacccgct tccatcgcgt ctaatgagct ttgagcggaa
     2401 actacggctt gtttgtaggc gttaatgcta ctgatagaag cattaatgtt gttgaaggag
     2461 gaacgtacgg tttgcacgac gctacgatgc gcgctttcca gttgctcgct ggcaccaaca
     2521 aagttgtact gggcctgttt cacctgcgag ttaaccattc cgccctgata aatcggcagc
     2581 gagaagctca ggccaacttt gttctggccc atattgctgt cgtcatactg ggtaccagcg
     2641 gcaccacggg ttttcgaacc gctataagag gtgtcagaaa tcccggtaga agccgttaaa
     2701 tccagagtcg gtaagtgacc atcctgcgcc tggcgaattt gctcgcgcgc caggtcctgg
     2761 ctcaagcgcg cctgtaacag cgacaggttg cgtttttcgg cttctttcag cagcgtgtta
     2821 accggctgtg gtttgtcggt tttaaagttt tcgacattca gcgccgccag ttctggatag
     2881 tagttaccgg tgatctggcg cagctgctct accgcgttat caaggttatt acgtgcggtc
     2941 acttcgttcg ccagcacggt atcgtactgt gcgcgggcgt tctgcacgtc agtgatcgct
     3001 accaggccca cgttaaaacg ttgggtggtt tgatctaatt gacggtagat cgcttctttt
     3061 tgcgcctgtg tataggaaag aacgtcaata gcattcaaca cgttgaaata agcggtcgcg
     3121 gtgttgagga tcaaggtttg ttgatcggtc tgatacgtga cgtcctgaat cccagctgct
     3181 ttttcctgca gcgttaacgc acgccatttc gacatatcaa aaatggattg agttaactgc
     3241 agggacgcac tggtcgcgtt agagttgatg ccgttcgcgt cgcggtagcc gttgctatag
     3301 gtgtaatctg cacctaaacc tagctgtggc agtaatggac tgcgcgcttc attaattttt
     3361 tcaaaggcag catcacgatc ggcggcagac ttacgcaatt ccgggttact aaggcgtgct
     3421 tgctgataaa cttgcatcag gttctcggcc tggctcaacg aactgaaacc agaaaggctc
     3481 aggccgataa gaatggggag caatttcttc atttgcattc cttgtggtga agcagtattt
     3541 agcgcgatca aactgtaaaa ttattgcgga ttctagcaga agccgctacc gcaaaaggtt
     3601 ggcgttacgt gccatagggc gttaatgtgc ctcaatttaa cacatgactg ctcaaacggc
     3661 agtcaaacgt cgctgaaatt cacatttaat tcactattag tgccaggaca ttaacaatgc
     3721 ttaagccaga caacctgccc gttacatttg gcaaaaacga tgtagaaatt attgcacgag
     3781 aaacacttta tcgcggcttt ttttcattag atctttatag atttcgtcat cgtctattca
     3841 acgggcaaat gagtcatgag gtacggcggg aaatttttga gcgcggtcac gccgcagtct
     3901 tgctaccctt tgacccagtg cgtgatgaag ttgtgctgat tgagcagatt cgaattgccg
     3961 cgtacgacac tagcgaaacc ccctggctac tggagatggt tgccgggatg attgaagagg
     4021 gtgaaagtgt ggaagatgtt gcccgtcgcg aagcgattga agaggcggga ctgatagtca
     4081 aacggaccaa accggtgtta agtttcctgg caagcccggg gggcaccagt gagcgttcgt
     4141 caattatggt gggcgaagtg gacgccacga ccgcaagcgg tattcacggt ctggctgatg
     4201 aaaacgaaga tattcgcgtt catgtggtaa gccgggaaca ggcataccag tgggtagaag
     4261 aggggaaaat cgacaacgca gcgtcggtca tcgctttgca atggctgcag ctgcatcatc
     4321 aagcgttaaa aaatgagtgg gcataaatga agcgttacac acctgacttt cctgaaatga
     4381 tgcgcctgtg cgagatgaac ttttcacaat tgcgccgttt gttaccgcgc aatgacgcac
     4441 ccggcgaaac tgtaagctat caggtggcaa acgcacaata tcggctgacg attgtggaat
     4501 cgacccgata cactaccctg gtgacaatag aacagactgc gcccgcgatc agttactgga
     4561 gccttccgtc aatgacggtg cgtctgtatc atgacgcgat ggtggctgaa gtgtgttcaa
     4621 gccagcagat ttttcgcttc aaagcgcggt atgattatcc taataaaaag ttgcatcaac
     4681 gcgacgaaaa gcatcaaatt aaccagtttt tagcggactg gttgcgatac tgtttagcac
     4741 atggagcgat ggcgattccg gtttactagc gtcgtgaaac ctaaggacac catttggaaa
     4801 gcctgttaac ccttcctctg gctggtgagg ccagagtcag gattttacaa attaccgaca
     4861 ctcacctgtt tgcacaaaag cacgaagccc tgttaggggt aaacacctgg gaaagctacc
     4921 aggcggtgct ggaggcgatt cgtccacacc agcacgaatt cgacctgatt gtcgcgacag
     4981 gtgatttagc gcaggatcaa tcctctgcgg cctatcagca tttcgctgaa ggcatcgcaa
     5041 gttttcgtgc gccctgcgtc tggctgccgg gcaaccacga tttccagccc gcgatgtaca
     5101 gcgcgttaca ggatgcgggt atctccccgg cgaagcgggt gtttattggt gagcaatggc
     5161 aaatcctgtt gctggatagc caggtgtttg gcgtgccgca cggtgagctg agcgagtttc
     5221 agcttgagtg gctggaacgt aaactggccg atgcgccaga acgccatacg ttgctgctgc
     5281 tgcatcatca tccgctacct gcgggttgta gttggcttga tcaacacagc ctgcgtaacg
     5341 cgggcgaact ggataccgtg ctggcgaagt ttccgcacgt caaatacttg ctgtgcggtc
     5401 atattcatca ggagctggat ctcgactgga acggtcgccg cctgctggca acgccgtcga
     5461 cctgtgtgca gtttaagccg cactgttcca actttacgct ggacaccatc gcgcccggct
     5521 ggcgtactct cgagttacat gctgatggca cgctgaccac cgaggtgcat cgcctggcgg
     5581 acacacgttt ccaacctgat accgcttcag aaggctactg atgtctacgc ttctttattt
     5641 acacggtttc aacagctcgc cgcgctctgc aaaagcgagc ttgttaaaaa actggcttgc
     5701 ggaacatcac cctgacgttg agatgatcat cccgcagttg ccgccgtatc cttccgatgc
     5761 ggcagaactg ctggaatcca ttgtcctgga acatggcggc gattcgctgg gtattgtcgg
     5821 ttcgtcactg gggggatatt acgccacctg gttgtcgcaa tgttttatgc tgcccgcagt
     5881 ggtggtaaac ccggcggtac gcccgtttga actgctgacg gactatctcg gtcagaacga
     5941 gaacccctac accgggcagc aatatgtgct agagtcacgc catatttacg atctcaaagt
     6001 catgcagatt gacccgctgg aagcgccgga tttgatctgg ctgctgcaac agacgggaga
     6061 tgaagtgctg gattaccgcc aggcggtggc gtactacgct tcctgccgcc agactgtcat
     6121 agaaggcggc aatcacgcat tcacgggctt cgaagattat ttcaacccga tcgtcgattt
     6181 tcttggtctg caccatctct gacgatcaac tcgaattgct aacttaaacc atgacgcaaa
     6241 cttataacgc tgatgccatt gaggtactca ccgggcttga gccagttcgc cgccgtccgg
     6301 ggatgtatac cgataccact cgccctaacc atttggggca agaagttatt gataacagtg
     6361 tcgatgaagc actggcgggt cacgcaaaac gcgtggacgt aatcttacat gctgaccagt
     6421 cgttagaagt gattgacgat gggcgcggga tgccggtgga tattcacccg gaagagggtg
     6481 taccggcggt tgaactgatt ctttgccgtc tgcatgcagg cggtaaattc tctaacaaaa
     6541 attaccagtt ctctggcggc ctgcatggcg tggggatttc ggtggttaac gccctgtcga
     6601 agcgcgtaga agttaacgta cgccgcgatg gtcaggttta taacatcgcc tttgaaaatg
     6661 gcgaaaaggt gcaggattta caggttgtcg gcacttgcgg taagcgcaat accggtacca
     6721 gcgtgcactt ctggccggat gaaaccttct ttgacagccc gcgtttttct gtttcacgcc
     6781 tgacgcatgt gctgaaggcc aaagcagtac tgtgccctgg tgttgagatc acctttaaag
     6841 atgagatcaa caacaccgaa cagcgctggt gctatcagga cggtctgaat gattacctgg
     6901 cggaagcggt aaacggttta ccgacgctgc cagaaaaacc gtttatcggt aatttcgctg
     6961 gcgatactga agcggtggac tgggcgctac tgtggctacc ggaaggcggt gaactgctga
     7021 ccgaaagcta cgtcaacctg atcccaacga tgcagggcgg tacccatgtt aatggcctgc
     7081 gtcagggcct gctggacgcg atgcgtgaat tctgtgaata ccgcaacatt ctgccgcgcg
     7141 gtgtaaagct gtcggcggaa gatatctggg atcgctgcgc ctatgtgctg tcagtaaaaa
     7201 tgcaggatcc gcagtttgcc gggcagacca aagaacgtct ctcttcgcgt cagtgtgcgg
     7261 catttgtttc tggcgtggtg aaagatgcct tcaccttgtg gctgaaccag aacgttcagg
     7321 cagctgaact gctggcggag atggcgattt ccagcgccca gcgtcgtatg cgtgcggcta
     7381 aaaaagtggt gcgtaaaaag ctaaccagcg gcccggcgct gcctggtaaa ttggctgact
     7441 gtaccgcgca ggatcttaac cgtaccgaac tattccttgt ggaaggtgac tccgcaggcg
     7501 gatctgccaa gcaggcgcgc gatcgcgaat atcaggcgat catgccgctg aaaggtaaga
     7561 tccttaacac ctgggaagtc tcttccgacg aagtgctggc ttcgcaggaa gtgcacgata
     7621 tttcggtagc gatcggtatc gatcctgaca gtgacgatct gagccaactt cgttacggca
     7681 agatctgtat cctggcggat gctgactccg atggtctgca cattgccacg ctgctctgcg
     7741 ctttgtttgt aaaacacttc cgcgcattgg tgaaacacgg tcacgtttac gttgcactgc
     7801 caccgctcta ccgtattgac ctcgggaaag aggtttatta cgcgctgacg gaagaagaga
     7861 aagagggcgt acttgagcaa ttaaaacgca agaaaggcaa gccaaacgtc cagcgtttta
     7921 aaggtctcgg ggaaatgaac ccgatgcaat tgcgcgaaac cacgcttgat ccgaacactc
     7981 gccgtctggt gcagttgact atcgatgatg aagacgatca gcgtactgac gcgatgatgg
     8041 atatgctgct ggcgaagaaa cgctcggaag atcgccgcaa ctggttgcaa gaaaaaggcg
     8101 acatggcgga gattgaggtc tgataatctt aacccggcaa acatgtgttt tgccgggttg
     8161 tcgttgatta ttttattggg cacagtttat tacgatctgg aaatagtgtt gccagcgaac
     8221 cagccagagt tttccagtcc gtagccattt tttctgcttg tgcagttgtt attactgcac
     8281 cttgttgttt aataagctca tcctccactc tggtatagtg aataatggta aaatccgtag
     8341 aggaccattt atccttagag aacatcttcg tcatcgcatg aatgtttgct tgcccgtcat
     8401 cgataaaaac aatcgcgtca tatttgttcc ccattttatc cagcatataa tcgagaatta
     8461 cgcctttatc ttgcccggaa gaaaaaaata ccccctggct gtacagccat gaccgtttga
     8521 gtttcccttt ttcaaagggt ggcaaagtat cctctttctt cctgagtgcg gaagacgaaa
     8581 aatcaatgcc gtttcgcttt aattcccgca atgtcggaaa gagagtatcc ggtgcacggg
     8641 atgtcagagc aaaagcggta tgcttttgtt gccactgttt taacattgtc ggtaactgtg
     8701 gctcggtaag ctgcataggg ctaagttcgt aaagcatact gattgtattt tcatacaggc
     8761 aaggaacttt ttgcgagtca tccggcttta aaggcaattt atcggtttgc cattgatacc
     8821 agatatcacc gccaatgcga gtgccactcg ttaataacgt gttatcaatg tcaaaaacca
     8881 ataatatctt atcggatgga tagagagttt ccttttctgt gataatttta ctgaccgcac
     8941 tatattcgcc agtaactata ttttttacct gtgcgtaaga taaagcaggc agagcagaga
     9001 gcatcatcca gagaatgatt ttaaatttat tattcatttt ttattccaga ttagaaagta
     9061 taagtcagac cccagcgcca gcggatttgg cgattacttg atgttttatc cacaccttta
     9121 taatccgcaa tactcatata gggtatccac gatcccattt tgtaccctac tttaaatgat
     9181 gcggtatagt ttttatggcc atcattccag gttaaataac cattattgta atagaccagg
     9241 tttaactgat attggaagtt ggttttttgt ggtcgatagt cgatccacac atcgtaacgg
     9301 cggttattac gatcatcgtt gccataacct ttggatttat cggtggcttt atccatgtca
     9361 taacgaaacc gacctgaaac tttccattct ttgctgaagt cataacccgc agaaagacca
     9421 tattcgtaag ttgaccaata ttgcgtggta tcaagccaga acatgggttc taaccagaat
     9481 tttttgtcat ccagcggctt aaagcggtaa acaacagata atcctgttcc gttattgcca
     9541 aattgtgagg ggtcccactt atctttatca ccttcatcgg tgttatagcg taattctccc
     9601 atcaaaccga taccattact aaaaaatgta ccaaattcaa cgcggtccgc gtgggtgcga
     9661 gtatcatcaa gatactcatg gcgataatca ataaatccat gcggctgttc tgcacagact
     9721 agtgtaggtg acaggcagga taacattgtt attcctgcaa gtgatatttt aagataagaa
     9781 ttcataatat tccctttcag aataaaat
//
LOCUS       DAZFLD010000173         9807 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.174, whole genome shotgun sequence.
ACCESSION   DAZFLD010000173 DAZFLD010000000
VERSION     DAZFLD010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9807
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.174"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            72..122
                     /gene="hisL"
                     /locus_tag="ACHKBL_003759"
     CDS             72..122
                     /gene="hisL"
                     /locus_tag="ACHKBL_003759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="his operon leader peptide"
                     /protein_id="HFO5820565.1"
                     /translation="MTRVQFKHHHHHHHPD"
     misc_feature    98..221
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00514"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="His leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00514"
     gene            268..1167
                     /gene="hisG"
                     /locus_tag="ACHKBL_003760"
     CDS             268..1167
                     /gene="hisG"
                     /locus_tag="ACHKBL_003760"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HFO5820566.1"
                     /translation="MTDNTRLRIAMQKSGRLSDDSRELLARCGIKINLHTQRLIAMAE
                     NMPIDILRVRDDDIPGLVMDGVVDLGIIGENVLEEELLNRRAQGEDPRYFTLRRLDFG
                     GCRLSLATPVDEAWDGPLSLNGKRIATSYPHLLKRYLDQKGISFKSCLLNGSVEVAPR
                     AGLADAICDLVSTGATLEANGLREVEVIYRSKACLIQRDGEMEESKQQLIDKLLTRIQ
                     GVIQARESKYIMMHAPTERLDEVIALLPGAERPTILPLAGDQQRVAMHMVSSETLFWE
                     TMEKLKALGASSILVLPIEKMME"
     gene            1173..2477
                     /gene="hisD"
                     /locus_tag="ACHKBL_003761"
     CDS             1173..2477
                     /gene="hisD"
                     /locus_tag="ACHKBL_003761"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HFO5820567.1"
                     /translation="MSFNTIIDWNSCTAEQQRQLLMRPAISASESITRTVNDILDNVK
                     ARGDEALREYSAKFDKTTVTALKVSAEEIAAASERLSDELKQAMAVAVKNIETFHTAQ
                     KLPPVDVETQPGVRCQQVTRPVASVGLYIPGGSAPLFSTVLMLATPARIAGCKKVVLC
                     SPPPIADEILYAAQLCGVQDVFNVGGAQAIAALAFGTESVPKVDKIFGPGNAFVTEAK
                     RQVSQRLDGAAIDMPAGPSEVLVIADSGATPDFVASDLLSQAEHGPDSQVILLTPDAD
                     MARRVAEAVERQLAELPRAETARQALNASRLIVTKDLAQCVEISNQYGPEHLIIQTRN
                     ARDLVDGITSAGSVFLGDWSPESAGDYASGTNHVLPTYGYTATCSSLGLADFQKRMTV
                     QELSKEGFSALASTIETLAAAERLTAHKNAVTLRVNALKEQA"
     gene            2474..3544
                     /gene="hisC"
                     /locus_tag="ACHKBL_003762"
     CDS             2474..3544
                     /gene="hisC"
                     /locus_tag="ACHKBL_003762"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HFO5820568.1"
                     /translation="MSTVTITDLARENVRNLTPYQSARRLGGNGDVWLNANEYPTAVE
                     FQLTQQTLNRYPECQPKAVIENYAQYAGVKPEQVLVSRGADEGIELLIRAFCEPGKDA
                     ILYCPPTYGMYSVSAETIGVECRTVPTLDNWQLDLQGISDKLDGVKVVYVCSPNNPTG
                     QLINPQDFRTLLELTRGKAIVVADEAYIEFCPQASLAGWLAEYPHLAILRTLSKAFAL
                     AGLRCGFTLANEEVINLLMKVIAPYPLSTPVADIAAQALSPQGIVAMRERVAQIIAER
                     EYLIAALKEISCVEQVFDSETNYILARFKASSAVFKSLWDQGIILRDQNKQPSLSGCL
                     RITVGTREESQRVIDALRAEQV"
     gene            3544..4611
                     /gene="hisB"
                     /locus_tag="ACHKBL_003763"
     CDS             3544..4611
                     /gene="hisB"
                     /locus_tag="ACHKBL_003763"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HFO5820569.1"
                     /translation="MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPELLKLQ
                     KAGYKLVMITNQDGLGTQSFPQADFDGPHNLMMQIFTSQGVQFDEVLICPHLPADECD
                     CRKPKVKLVERYLAEQAMDLANSYVIGDRATDIQLAENMGINGLRYDRETLNWPMIGE
                     QLTKRDRYAHVVRNTKETQIDVQVWLDREGGSKINTGVGFFDHMLDQIATHGGFRMEI
                     NVKGDLYIDDHHTVEDTGLALGEALKIALGDKRGICRFGFVLPMDECLARCALDISGR
                     PHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLHLKTKGKNDHHRVESLFKAFG
                     RTLRQAIRVEGDTLPSSKGVL"
     gene            4611..5201
                     /gene="hisH"
                     /locus_tag="ACHKBL_003764"
     CDS             4611..5201
                     /gene="hisH"
                     /locus_tag="ACHKBL_003764"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HFO5820570.1"
                     /translation="MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDVVLLADKLFL
                     PGVGTAQAAMDQVRERELFDLIKACTQPVLGICLGMQLLARRSEESNGVDLLGIIDED
                     VPKMTDFGLPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYG
                     EPFTAAVQKDNFYGVQFHPERSGAAGAKLLKNFLEM"
     gene            5201..5938
                     /gene="hisA"
                     /locus_tag="ACHKBL_003765"
     CDS             5201..5938
                     /gene="hisA"
                     /locus_tag="ACHKBL_003765"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HFO5820571.1"
                     /translation="MIIPALDLIDGTVVRLHQGDYGKQRDYGNDPLLRLQDYAAQGAE
                     VLHLVDLTGAKDPARRQIPLIKTLVADVNVPVQVGGGVRSEEDVAALLEAGVARVVVG
                     STAVKSPEMVKGWFERFGADALVLALDVRIDEQGNKQVAVSGWQENSGVSLEQLVETY
                     LPVGLKHVLCTDISRDGTLAGSNVSLYEEVCARYPQVAFQSSGGIGDINDVAALRGTG
                     VRGVIVGRALLEGKFTVKEAIACWQNA"
     gene            5920..6696
                     /gene="hisF"
                     /locus_tag="ACHKBL_003766"
     CDS             5920..6696
                     /gene="hisF"
                     /locus_tag="ACHKBL_003766"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HFO5820572.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGA
                     DELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVAGGIKSLEDAAKILSFGADKISI
                     NSPALADPTLITRLADRFGVQCIVVGIDTWYDAETGKYHVNQYTGDESRTRVTQWETL
                     DWVQEVQKRGAGEIVLNMMNQDGVRNGYDLEQLKKVREVCHVPLIASGGAGTMEHFLE
                     AFRDADVDGALAASVFHKQIINIGELKAYLATQGVEIRIC"
     gene            6690..7301
                     /gene="hisIE"
                     /locus_tag="ACHKBL_003767"
     CDS             6690..7301
                     /gene="hisIE"
                     /locus_tag="ACHKBL_003767"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HFO5820573.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TLESGKVTFFSRTKQRLWTKGETSGNFLNVVSIAPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGDTAHQWLFLYQLEQLLAERKSADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLRKRHQ"
     gene            complement(7396..8373)
                     /gene="wzzB"
                     /locus_tag="ACHKBL_003768"
     CDS             complement(7396..8373)
                     /gene="wzzB"
                     /locus_tag="ACHKBL_003768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310855.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
                     /protein_id="HFO5820574.1"
                     /translation="MRVENNNVSGQNHDPEQIDLIDLLVQLWRGKMTIIISVIVAIAL
                     AIGYLAVAKEKWTSTAIVTQPDVGQIAGYTNAMNVIYGSAVPKVSDIQASLIGRYSTA
                     FSALAETLDNQEEAEKLTIEPTVKNQSLPLAVSYVGETPEGAQKQLAKYIQQVDDQVN
                     EELEKDLKDNIALRMKNLQDSLRTQEVVAQEQKDLRIRQIQEALQYANQAQVTKPQVQ
                     QTEDVTQDTLFLLGSEALESMIKHEATRPLVFSPNYYQTRQNLLDIENLKVDDLDIHA
                     YRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLGRNALRNYNAK"
     gene            complement(8520..9686)
                     /gene="ugd"
                     /locus_tag="ACHKBL_003769"
     CDS             complement(8520..9686)
                     /gene="ugd"
                     /locus_tag="ACHKBL_003769"
                     /EC_number="1.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="HFO5820575.1"
                     /translation="MKITISGTGYVGLSNGLLIAQNHEVVALDILPSRVAMLNDRISP
                     IVDKEIQQFLQSDKIHFNATLDKNEAYRDADYVIIATPTDYDPKTNYFNTSSVESVIK
                     DVVEINPYAVMVIKSTVPVGFTEAMHKKYRTENIIFSPEFLREGKALYDNLHPSRIVI
                     GERSERAERFAALLQEGAIKQNIPTLFTDSTEAEAIKLFANTYLAMRVAYFNELDSYA
                     ESLGLNTRQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYQSVPNNLISAI
                     VDANRTRKDFIADAILSRKPQVVGIYRLIMKSGSDNFRASSIQGIMKRIKAKGVEVII
                     YEPVMKEDSFFNSRLERDLATFKQQADVIISNRMAEELKDVADKVYTRDLFGSD"
BASE COUNT         2428 a         2523 c         2575 g         2281 t
ORIGIN      
        1 cccttgcttt ctaacgtgaa agtggtttag gttaaaagac atcagttgaa taaacattca
       61 cagagacttt tatgacacgc gttcaattta aacaccacca tcatcaccat catcctgact
      121 agtctttcag gcgatgtgtg ctggaagaca ttcagatctt ccagtggtgc atgaacgcat
      181 gagaaagccc ccggaagatc accttccggg ggctttttta ttgcgcggtt gataacggtt
      241 cagacaggtt taaagaggaa taagaaaatg acagacaaca ctcgtttacg catagctatg
      301 cagaaatccg gccgtttaag tgatgactca cgcgaattgc tggcgcgctg tggcattaaa
      361 atcaatcttc acacccagcg cctgatcgcg atggcagaaa acatgccgat tgatattctg
      421 cgcgtgcgtg acgacgacat tcccggtctg gtaatggacg gcgtggtaga ccttgggatt
      481 atcggcgaaa acgtgctgga agaagagctg cttaaccgcc gagcccaggg tgaagatccg
      541 cgctacttta ccctgcgtcg tctggatttc ggcggctgcc gcctttcgct ggcaacgccg
      601 gttgatgaag cctgggatgg cccgctctcc ttaaacggta aacgtatcgc cacctcttat
      661 cctcacctgc tcaagcgtta tctcgaccag aaaggcatct cttttaaatc ctgcttactg
      721 aacggttctg ttgaagtcgc cccgcgtgcc ggactggcgg atgcgatttg cgatctggtt
      781 tccaccggtg ccacgctgga agcaaacggc ctgcgcgaag tcgaagttat ctatcgctcg
      841 aaagcttgcc tgatacaacg cgatggcgaa atggaagaat ccaaacagca gctgatcgac
      901 aaactgctga cccgtattca gggcgtgatc caggcgcgcg aatcaaaata catcatgatg
      961 cacgcaccga ccgaacgcct ggatgaagtc atcgccctgc tgccaggtgc cgaacgccca
     1021 actattctgc cgctggcagg tgatcaacag cgcgtagcga tgcacatggt gagtagcgaa
     1081 accctgttct gggaaacgat ggaaaaactg aaagcgctgg gtgccagttc aatcctggtc
     1141 ctgccgattg aaaaaatgat ggagtgatcg ccatgagctt taacacaatc attgactgga
     1201 atagctgtac tgcggagcaa caacgccagc tgttaatgcg cccggcgatc tccgcttctg
     1261 aaagcattac ccgcaccgtt aacgatatcc tcgataacgt gaaagcacgc ggcgatgagg
     1321 ccctgcgtga atacagcgcg aaatttgata aaaccacggt taccgcgctg aaggtgtctg
     1381 ctgaggagat cgccgccgcc agcgaacgcc tgagcgacga gctaaaacag gcgatggcgg
     1441 tggcagtaaa gaatattgaa accttccaca ctgcgcaaaa actgccgccg gtagatgtcg
     1501 aaacgcagcc aggcgtgcgt tgccagcaag tcacgcgccc ggtcgcttca gttgggttgt
     1561 atattcctgg cggctccgcc ccgctcttct caacggtatt aatgctggca actccggcgc
     1621 gtattgcggg ctgtaaaaaa gtggtgctgt gctcaccgcc gccgattgcc gacgaaattc
     1681 tttacgcggc gcagctgtgc ggtgtgcagg atgtgtttaa cgtcggcggc gcacaggcca
     1741 ttgccgcact ggcgtttggt acggaatctg tgccgaaagt tgacaaaatc ttcgggccag
     1801 gtaacgcctt tgtcaccgag gcaaaacgtc aggtgagcca gcgtctggac ggtgcagcga
     1861 tcgatatgcc cgcaggcccg tcggaagtgc tggtgattgc tgacagcggc gctacgccgg
     1921 atttcgtggc ttctgatttg ctttctcagg ctgaacacgg cccggactcg caggtgattt
     1981 tactgacgcc cgatgctgat atggcgcgtc gcgttgccga ggctgtcgaa cgccaactgg
     2041 cagaactgcc gcgtgccgaa accgcccgcc aggcactgaa cgccagccgc ctgatcgtga
     2101 ctaaagattt agcgcagtgc gtagagatct ccaaccagta cggcccggag cacctgatca
     2161 tccagacccg caacgcccgc gatctggtcg atggcatcac cagcgccggt tcggtatttc
     2221 ttggtgactg gtcacccgaa tcggcgggtg attacgcctc cggcaccaac cacgtactgc
     2281 cgacttacgg ttacaccgcc acctgttcca gcctcgggct ggcagatttc cagaagcgta
     2341 tgaccgtgca ggaactgtcg aaagaaggtt tctccgcgct ggcttcgacc atagaaacac
     2401 tggccgccgc cgagcgcctg accgcccata aaaatgccgt tactttgcgt gttaacgccc
     2461 ttaaggagca agcatgagca ccgtgactat taccgattta gcgcgtgaaa acgtccgcaa
     2521 cctgacgccg tatcagtcgg cgcgtcgtct gggcggtaac ggcgacgtct ggctgaacgc
     2581 caacgaatac cccacagccg tggagtttca gctcactcaa caaacgctca accgctatcc
     2641 ggaatgtcag ccaaaagcgg tgattgaaaa ttacgcgcaa tatgcaggcg taaaaccgga
     2701 acaggtgctg gtcagccgtg gcgcggacga aggtattgaa ctgctgattc gcgctttttg
     2761 cgaaccgggt aaagacgcca tcctctactg cccgccaacg tacggcatgt acagcgtcag
     2821 cgctgaaacc attggcgtcg agtgccgcac agtgccgacg ctggacaact ggcaactgga
     2881 cttacagggc atttccgaca agctggacgg cgtaaaagtg gtctatgttt gcagccccaa
     2941 caacccgacc gggcaactga tcaacccgca ggattttcgc actctgctgg agttaacccg
     3001 cggtaaagcg attgtggttg ccgatgaagc ctatatcgag ttttgcccgc aggcatcgct
     3061 ggctggctgg ctggcggaat atccgcacct ggctattttg cgcacactgt cgaaagcttt
     3121 tgctctggcg ggccttcgtt gcggatttac gctggcaaac gaagaagtca tcaaccttct
     3181 gatgaaagtg atcgccccct acccgctctc gacgccggtc gccgacattg cggcccaggc
     3241 gttaagcccg caggggatcg tcgccatgcg cgaacgggtg gcgcaaatta ttgcagaacg
     3301 cgaatatctg attgccgcat tgaaagaaat ctcctgcgtg gagcaggttt ttgactctga
     3361 aaccaactac attctggcgc gctttaaagc ctccagcgca gtgtttaaat ctttgtggga
     3421 tcagggcatt atcttacgtg atcagaataa acaaccctct ttaagcggct gcctgcgaat
     3481 taccgtcgga acccgtgaag aaagccagcg cgtcattgac gccttacgtg cggagcaagt
     3541 ttgatgagtc agaagtatct ttttatcgat cgcgatggaa ccctgattag cgaaccgccg
     3601 agtgattttc aggtggaccg ttttgacaaa ctcgcctttg aaccgggcgt gatcccggag
     3661 ctgctgaagc tgcaaaaagc gggatataag ctggtgatga tcaccaatca ggatggtctg
     3721 ggaacacaaa gtttcccgca ggcggatttt gatggcccgc acaacctgat gatgcagatc
     3781 ttcacctcgc aaggcgtgca gtttgatgaa gtgctgattt gcccgcacct gcccgccgat
     3841 gagtgcgact gccgtaaacc gaaagtaaaa ctggtggagc gttatctcgc tgagcaagcg
     3901 atggatctcg ccaacagtta tgtgattggc gatcgcgcga ccgatattca actggcggaa
     3961 aacatgggta ttaatggttt acgctacgac cgcgaaaccc tgaactggcc gatgattggc
     4021 gagcaactca ctaaacgaga ccgttacgcc catgtagtgc gcaacaccaa agagacgcag
     4081 attgacgttc aggtatggct ggatcgcgaa ggtggcagca agattaacac cggcgttggc
     4141 ttctttgatc atatgctgga tcagatcgcc actcacggcg gtttccgcat ggaaatcaac
     4201 gtcaaaggcg acctctatat cgacgatcac cacaccgtcg aagataccgg cctggcgctg
     4261 ggtgaagcgt taaaaattgc cctcggcgat aaacgcggta tttgccgctt tggttttgtg
     4321 ctgccgatgg acgaatgcct tgcccgctgt gcactggata tctctggtcg cccacacctg
     4381 gaatataaag ctgaatttac ctaccagcgc gtcggcgatc tcagcaccga gatgatcgag
     4441 cacttcttcc gttcgctctc ttacaccatg ggcgtgacgt tgcacctgaa aaccaaaggt
     4501 aaaaacgatc atcaccgtgt agagagtctc tttaaagcct ttggtcgcac cctgcgccag
     4561 gccatccgcg tggaaggcga caccctgccc tcgtcgaaag gagtgctgta atgaacgtgg
     4621 tgatccttga taccggctgc gccaatttga actcggtgaa gtcagccatt gcgcgtcacg
     4681 gttatgaacc caaagtcagc cgtgacccgg acgtcgtgtt gctggccgat aaactatttt
     4741 tacccggcgt aggcaccgca caagcagcga tggatcaggt gcgtgagcgc gagctgtttg
     4801 atctcatcaa agcctgtacc caaccggtgc tgggcatctg cttagggatg caactgctgg
     4861 cgcggcgcag cgaagagagc aacggcgtcg atttgctggg cattatcgac gaagacgtgc
     4921 cgaaaatgac cgactttggt ctgcccctgc cgcatatggg ctggaaccgc gtttacccgc
     4981 aggcaggcaa ccgcctgttt caggggattg aagacggcgc gtacttttac tttgttcaca
     5041 gctacgcaat gccagtcaat ccgtggacca tcgcccagtg taattacggc gaaccgttca
     5101 ccgcggcggt acaaaaagat aacttctacg gcgtgcagtt ccacccggag cgttctggtg
     5161 ccgctggcgc taagctgctg aaaaacttcc tggagatgtg atgattattc cggcattaga
     5221 tttaatcgac ggcaccgtgg tgcgtctcca tcagggagat tatggcaaac aacgcgatta
     5281 cggtaacgac ccgctgctac gcttacagga ttacgccgcg cagggtgccg aagtgttgca
     5341 tctggtggat ctgaccgggg caaaagatcc ggctagacgt caaatcccgc tgattaaaac
     5401 cctggtggcg gacgttaacg ttccggtgca ggttggcggc ggcgtgcgta gcgaagaaga
     5461 cgtggcggcg ttactggaag cgggcgttgc gcgcgtggtg gttggctcca ccgcggtgaa
     5521 atcaccagaa atggtgaaag gctggtttga acgtttcggt gccgatgcct tagtgctggc
     5581 gctggatgtc cgtattgacg agcaaggcaa caagcaggtg gcggtgagcg gctggcaaga
     5641 gaactcgggc gtttcactgg aacaactggt ggaaacctat cttcccgtcg gcctgaaaca
     5701 tgtgctatgt accgatatct cgcgcgacgg cacgctggca ggttctaacg tctctttata
     5761 tgaagaagtg tgcgccagat acccgcaggt ggcatttcag tcctccggcg gcattggcga
     5821 cattaacgat gttgctgccc tgcgaggtac tggcgtgcgc ggcgtaatag ttggtcgggc
     5881 attactggaa ggtaaattca ccgtgaagga ggctatcgca tgctggcaaa acgcataatt
     5941 ccatgtctcg acgttcgtga tggtcaggtg gtgaaaggcg tacagtttcg caaccatgaa
     6001 atcattggcg atatcgtgcc gctggcaaaa cgttacgccg aagaaggtgc agacgaactg
     6061 gtgttctacg atatcaccgc ttccagcgat ggccgtgtgg tagataaaag ctgggtatct
     6121 cgcgtggcgg aagtaatcga cattccgttt tgtgtggcgg gtgggattaa gtctctggaa
     6181 gatgccgcga aaattctttc ctttggcgcg gataaaatct ccattaactc ccctgcactg
     6241 gcagacccga cgttaattac tcgcctggcg gatcgctttg gcgtgcagtg tattgtggtc
     6301 ggcattgata cctggtacga cgccgaaacc ggtaaatatc atgtgaatca atataccggc
     6361 gatgaaagcc gcacccgcgt cactcagtgg gaaacactcg actgggtgca ggaagtgcaa
     6421 aaacgcggtg ccggagaaat cgtcctcaac atgatgaatc aggacggcgt gcgtaacggt
     6481 tacgacctcg aacagctgaa aaaagtgcgt gaagtttgcc acgtcccgct gattgcctcc
     6541 ggtggcgcgg gcaccatgga acacttcctg gaagccttcc gcgatgccga cgttgacggt
     6601 gcgctggctg cttccgtatt ccacaaacaa ataatcaata ttggtgaatt aaaagcgtac
     6661 ctggcaacac agggcgtgga gatcaggata tgttaacaga acaacaacgt cgcgaactgg
     6721 actgggaaaa aaccgacgga ctgatgccgg tgattgtgca acatgcggta tccggcgaag
     6781 tgctaatgct gggctatatg aacccggaag ctctggacaa aaccctcgaa agcggcaaag
     6841 tcaccttctt ctcgcgcact aaacagcgac tgtggaccaa aggcgaaacg tcgggcaatt
     6901 ttctcaatgt ggtgagtatt gccccggact gcgacaacga cacgttactg gtgctggcga
     6961 atcccatcgg gccaacctgc cacaaaggca ccagcagctg cttcggcgac accgctcacc
     7021 agtggctgtt cctgtatcaa ctggaacaac tgctcgccga gcgcaaatct gccgatccgg
     7081 aaacctccta caccgccaaa ctgtatgcca gcggcaccaa acgcattgcg cagaaagtgg
     7141 gcgaagaagg cgtggaaacc gcactggcag caacggtaca tgaccgcttt gaactgacca
     7201 acgaggcatc tgatttgatg tatcacctgc tggtgctgtt gcaggatcag gatctggatt
     7261 taacgacggt aattgagaac ctgcgtaaac ggcatcagtg agttgcgggt gatcagatgc
     7321 gatgttgttg tcgcatccgg caaaaaaaac gggcaaggtg tcaccaccct gcccgttttc
     7381 tttaaaacca aaagattact tcgcgttgta attacgcaga gcgttacgcc ccagcacaat
     7441 ccccgcgcca accatgccgc ccagcagcac tgccagaatc aaggtaattg cctttttcgg
     7501 gctatcgcga cgaataggta acgtcggttt catcacatag cggtaagcat gaatatcaag
     7561 atcatcaacc tttaagtttt caatatccaa caggttttga cgagtctgat agtagtttgg
     7621 cgagaacacc aacggacgag tcgcctcatg cttaatcatc gactccagcg cttcgctccc
     7681 caaaaggaac aacgtatctt gtgtcacatc ttcagtctgc tgaacctgtg gctttgtcac
     7741 ctgcgcctga ttcgcatact gcaacgcttc ctgaatctga cggatacgca gatctttctg
     7801 ctcttgcgcg actacttcct gagttcttaa agagtcctgc aagtttttca tacgcagcgc
     7861 gatgttgtct ttcaggtctt tttccagttc ctcattaacc tggtcgtcaa cttgctggat
     7921 atacttcgcc agctgcttct gagcaccttc aggtgtttca ccaacataag acaccgctaa
     7981 aggtaatgac tgatttttaa cggtaggctc aatggtcagc ttttctgctt cttcctgatt
     8041 atccagcgtt tccgctaatg ctgaaaatgc agtgctgtaa cgtccgataa gtgaagcctg
     8101 aatgtcggag actttcggta cagccgatcc gtagatcaca ttcatcgcgt tggtataacc
     8161 agcgatttgc cccacatcag gttgagtaac gatagctgtg gaagtccact tttctttggc
     8221 aacggccaga taccctatag ccagggcaat agcaacaatg acagaaatga tgatagtcat
     8281 cttgccgcgc cacaactgca ccagtaaatc aatcaaatca atctgttccg ggtcatggtt
     8341 ttgcccagaa acattattat tttctactct catcattacc ctaacggacg aaataatttt
     8401 aagtcgccat agtttagcga taatcgccat gaatgctata ggataaatga taaaaagaat
     8461 gaattattac aaagggaaca tgagagaatc agggcgaaaa tcgccctgat aacaggatgt
     8521 tagtcactgc caaagagatc gcgggtgtag actttgtctg ccacatcctt aagctcttct
     8581 gccatacggt tggaaataat aacgtcggct tgttgtttga aggtggcgag atcacgttcc
     8641 agtcgagagt tgaagaatga atcttctttc atcaccggct cgtagatgat cacttcaaca
     8701 ccttttgcct tgatacgttt cataatcccc tgaatggaag acgcgcggaa gttatctgac
     8761 ccgctcttca taatcagacg ataaataccc accacttgcg gtttgcgtga caaaatggca
     8821 tcggcaataa aatctttacg cgtgcggttt gcatcgacaa ttgccgagat caggttattc
     8881 ggcacagact ggtagttcgc cagtaactgc ttggtatctt tcggtagaca ataaccacca
     8941 tagccaaacg acggattgtt gtaatggttg ccaatacgcg gatcgagaca aacaccttcg
     9001 ataatctgac gagtattcag acctaaactt tctgcatagc tatccagctc gttaaagtat
     9061 gctacgcgca tcgccagata ggtattagca aacagtttaa tcgcttctgc ttcagtggag
     9121 tcggtaaaca gggtcgggat attttgctta atcgcgcctt cctgtaataa cgccgcaaaa
     9181 cgttctgcgc gttctgaacg ctcaccgatg acaatacgtg acggatgaag gttatcgtaa
     9241 agagctttac cttcacggag aaattccgga gagaaaataa tattttcagt acgatatttc
     9301 ttatgcatcg cttcggtaaa accaacggga accgttgatt tgatgaccat aaccgcataa
     9361 ggatttatct caactacgtc tttaattact gattctacac tcgatgtatt gaaataatta
     9421 gttttaggat catagtcggt tggagtggcg atgatgacgt aatcagcatc ccgataggct
     9481 tcatttttat ctaatgtggc attaaagtgt attttatctg attgcaaaaa ctgctgaatt
     9541 tccttatcaa caataggaga tatccgatca ttcagcatag caacgcgtga cggtaaaata
     9601 tctaatgcca caacctcatg attttgtgcg attagaagcc cgtttgacaa gccgacatag
     9661 ccagtaccgg aaatggtgat tttcatttca tgctctcaga attaacttaa ctgtgaatca
     9721 tgatgttttt agcatcctga taagagttaa aagttttaac gctacgttgt aagatggtca
     9781 acgcaacgaa aaacaattca gaatttc
//
LOCUS       DAZFLD010000174         9798 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.175, whole genome shotgun sequence.
ACCESSION   DAZFLD010000174 DAZFLD010000000
VERSION     DAZFLD010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9798
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.175"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..716)
                     /gene="phoU"
                     /locus_tag="ACHKBL_003770"
     CDS             complement(<1..716)
                     /gene="phoU"
                     /locus_tag="ACHKBL_003770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HFO5820576.1"
                     /translation="MDSLNLNKHISGQFNAELESIRTQVMTMGGMVEQQLSDAITAMH
                     NQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKTIAELERI
                     GDVADKICRTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYRE
                     DKKVDQEYEGIVRQLMTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICEFIFYYVK
                     GQDFRHVGGDELDKLLAEKD"
     gene            complement(731..1504)
                     /gene="pstB"
                     /locus_tag="ACHKBL_003771"
     CDS             complement(731..1504)
                     /gene="pstB"
                     /locus_tag="ACHKBL_003771"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFO5820577.1"
                     /translation="MSMVETAPSKIQVRNLNFYYGKFHALKNINLDIAKNQVTAFIGP
                     SGCGKSTLLRTFNKMFELYPEQRAEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTP
                     FPMSIYDNIAFGVRLFEKLSRADMDERVQWALTKAALWNETKDKLHQSGYSLSGGQQQ
                     RLCIARGIAIRPEVLLLDEPCSALDPISTGRIEELITELKQDYTVVIVTHNMQQAARC
                     SDHTAFMYLGELIEFSNTDDLFTKPAKKQTEDYITGRYG"
     gene            complement(1595..2485)
                     /gene="pstA"
                     /locus_tag="ACHKBL_003772"
     CDS             complement(1595..2485)
                     /gene="pstA"
                     /locus_tag="ACHKBL_003772"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HFO5820578.1"
                     /translation="MAMVEMQTTAALAESRRKMQARRRLKNRIALTLSMATMAFGLFW
                     LIWILMSTITRGIDGMSLALFTEMTPPPNTEGGGLANALAGSGLLILWATVFGTPLGI
                     MAGIYLAEYGRKSWLAEVIRFINDILLSAPSIVVGLFVYTIVVAQMEHFSGWAGVIAL
                     ALLQVPIVIRTTENMLKLVPDSLREAAYALGTPKWKMIAAITLKASVSGIMTGILLAI
                     ARIAGETAPLLFTALSNQFWSTDMMQPIANLPVTIFKFAMSPFAEWQQLAWAGVLIIT
                     LCVLLLNILARVVFAKNKHG"
     gene            complement(2485..3444)
                     /gene="pstC"
                     /locus_tag="ACHKBL_003773"
     CDS             complement(2485..3444)
                     /gene="pstC"
                     /locus_tag="ACHKBL_003773"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HFO5820579.1"
                     /translation="MAATKPAFNPPGKKGDIIFSVLVKLAALTVLLMLGGIIVSLIIS
                     SWPSIQKFGLAFLWTKEWDAPNDIYGALVPIYGTLVTSFIALLIAVPVSFGIALFLTE
                     LAPGWLKRPLGIAIELLAAIPSIVYGMWGLFIFAPLFAVYFQEPVGNIMSNIPIVGAL
                     FSGPAFGIGILAAGVILAIMIIPYIAAVMRDVFEQTPVMMKESAYGIGCTTWEVIWRI
                     VLPFTKNGVIGGIMLGLGRALGETMAVTFIIGNTYQLDSASLYMPGNSITSALANEFA
                     EAESGLHVAALMELGLILFVITFIVLAASKFMIMRLAKNEGAR"
     gene            complement(3531..4571)
                     /gene="pstS"
                     /locus_tag="ACHKBL_003774"
     CDS             complement(3531..4571)
                     /gene="pstS"
                     /locus_tag="ACHKBL_003774"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HFO5820580.1"
                     /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW
                     ADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDAPLSDEKLAQEGLFQFPTVIG
                     GVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDEAIAKLNPGLKLPSQNIAVVRR
                     ADGSGTSFVFTSYLAKVNEEWKNNVGTGSTVKWPIGLGGKGNDGIAAFVQRLPGAIGY
                     VEYAYAKQNNLAYTKLISADGKPVSPTEENFANAAKGADWSKTFAQDLTNQKGEDAWP
                     ITSTTFILIHKDQKKPEQGTEVLKFFDWAYKTGAKQANDLDYASLPDSVVEQVRAAWK
                     TNIKDSSGKPLY"
     gene            complement(4819..5889)
                     /locus_tag="ACHKBL_003775"
     CDS             complement(4819..5889)
                     /locus_tag="ACHKBL_003775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO5820581.1"
                     /translation="MNKYIIPWCFIVFIFSFSGATFAAPKGICTSDNGSFHSTLDFSG
                     YLITADENRVGKTFNKTVTNGDSYPAHCHCDTGNVGEFPYIYYTARINESLGYAGVRS
                     NVNYFNLNPNLDVGISIDLLGVGYINAPFEYHANRPTGGSYKCSRTDPLSISSGAKAI
                     IYFYIKKTFAGKLIIPETLVAKLYGTISRDTPVDYSQPMADVYIRGDITAPQSCEINS
                     LRPINFDFKEIPAADFSSVVGSTVTTHKITKTVTIECENLGILNTDDISTSFYATEPS
                     TDNSMVVTSNPNVGIKIYDKNNKEINVNGGELPTDMGKSTVFGEKAGSVTFSAAPASL
                     TGARPAPGTFTATATITIEIVR"
     gene            complement(5900..8422)
                     /gene="lpfC-O113"
                     /locus_tag="ACHKBL_003776"
     CDS             complement(5900..8422)
                     /gene="lpfC-O113"
                     /locus_tag="ACHKBL_003776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial usher protein LpfC-O113"
                     /protein_id="HFO5820582.1"
                     /translation="MMTTRIVVGLTAGTCLIFSQSLMAEVSVFNPALLEIDHQSGVDI
                     RQFNRANLMPPGVYSVDIFINGKMFERQDVTFVQDNPDADLHACFVAIKKTLTTFGVK
                     VDALKSLNDVDETVCIDPGPRIEGSSWQFDSDKLQLNISIPQIYMDAMAYDYISPSRW
                     DEGINALTINYDFSGSHTLRSDYGSQETDTSYLNLRNGLNIGPWRLRNYSTLNTTDGS
                     AEYNSISTWIQRDIAALRSQIMIGDTWTASDIFDSTQIRGARLYTDNDMLPASQNGFA
                     PVVRGIAKSNATVIIRQNGYVIYQSAVPQGAFEITDLNTASTGGDLDVTIKEEDGSEQ
                     RFTQPYASLAILKREGQTDVDVSVGELRDEDGFTPDVLQVQILHGFPYGFTLYGGMQA
                     AEKYGSAALGVGKELGALGAISFDVTHARAKFSHADTETGQSYRFLYSKRFDDTDTSL
                     RLVGYRYSTEGYYTLNEWASRRNNPEDFWETGNRRSRVEGTLTQSLGRDYGNLYLTLS
                     RQQYWHTDDVERLMQFGYSSSWKRLSWNVSWSYSNTARQGTGNNHASDNTSEQIYMLS
                     LSVPLSGWWGNSYATYSVSQNDNSGSSHQLGLSGTALERNNLSWNLMQSYNSHDDEVG
                     GNMSLTYDGTYGTVNGSYNYSQNSQRLNYGIRGGILAHSEGVTLSQELGETIALVKAP
                     GAAGLEIDNMRGAATDWRGYTVKTQLNPYDENRVAISDNYFSKSNIELDNTVVTMVPT
                     RGAVVKAEFVTRVGYRVLFRVAGTKGKPAPFGAIATVQNTSSADSGIVGDQGELYLSG
                     LPEKGQVMLSWGENAATTCTFDYSLSIPESESGLIEQGVTCH"
     gene            complement(8447..9178)
                     /gene="lpfB-O113"
                     /locus_tag="ACHKBL_003777"
     CDS             complement(8447..9178)
                     /gene="lpfB-O113"
                     /locus_tag="ACHKBL_003777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_316045823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial chaperone LpfB-O113"
                     /protein_id="HFO5820583.1"
                     /translation="MSRKSCLIRLFKTTVSCKLFTAIILSVFLVQPALTYAGVVIGGT
                     RVVYLSNNPDKSISVFSKEEKIPYLIQAWVDPFNKEDKSKAPFTVIPPVSRLEPAQEK
                     VLRIIHTKGVSLPDDRESVFWLNIKNIPPSASNKAANSLEIAVKTRIKLFWRPASIRL
                     IPEDAAPKVKWHREGRNLIAENPNPIHISVMDVIVDGHDVPLNMIRPFETLTLPLPAN
                     SAGGQMTWRFINDYGAVSDPIKMTL"
     gene            complement(9226..9798)
                     /gene="lpfA-O113"
                     /locus_tag="ACHKBL_003778"
     CDS             complement(9226..9798)
                     /gene="lpfA-O113"
                     /locus_tag="ACHKBL_003778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbria major subunit LpfA-O113"
                     /protein_id="HFO5820584.1"
                     /translation="MKRNIIGGAFTLASLMLAGHALAEDGVVHFVGEIVDTTCEVTSD
                     TADQTVPLGKVSKNAFSGVGSLASPQQFSIKLEKCPATYTQAAVRFDGTEAPGGDGDL
                     KVGTPLTAGNPGDFTGTGQAIAATGVGIRIFNQSDNSQVKLYNDSAYTAIDAEGKAEM
                     KFIARYVATNATVTAGTANADSQFTVEYKK"
BASE COUNT         2503 a         2502 c         2307 g         2486 t
ORIGIN      
        1 ctatcttttt ctgccagcag tttatcgagc tcgtcaccac cgacgtgacg gaaatcctgc
       61 cccttcacgt agtagaagat aaactcacaa atattctggc agcggtcgcc gatacgttcg
      121 attgaacgcg cacaaaacag cgcggtaagt acgcttggaa tggtgcgcga atcttccatc
      181 atgtaggtca tcagctgacg aacaatacct tcgtactcct gatcgacttt tttatcttca
      241 cgataaatac gtaccgcttc atcaatgtcc atccgcgcga acgcgtccag cacgtcgtgc
      301 agcatctgga tggtatgacg gcccagcgac tccagactta ccagcaatgg ctgatgctgc
      361 tgggagaatt tctccagtgc agtacggcag attttgtccg ccacgtcgcc aatacgctcc
      421 agctcggcaa tggttttact gatcaccata accagacgca ggtcgctcgc cgtcggctgg
      481 cgtttggcaa taatgcgcac gcaggcttca tcgatcgcca cttccatcat gttgacgttc
      541 ttgtcgcctt cgatgacgcg cttcgccaga tcgctgtcct ggttgtgcat tgcagtgatc
      601 gcatcagaaa gctgctgctc caccatgccg cccatggtca tcacctgcgt gcggatgctt
      661 tccagttcgg cgttgaactg gccggaaata tgtttattaa gattgagact gtccataacg
      721 cactcctgaa tcaaccgtaa cgaccggtga tgtagtcttc tgtttgtttc ttcgctggct
      781 tggtgaacag atcgtccgtg ttgctgaact caatcaattc gcccaggtac ataaacgccg
      841 tgtggtcgga acaacgcgca gcctgctgca tgttgtgggt gacgatcacc acggtgtaat
      901 cctgcttcag ttcggtaatc agctcttcaa tacgcccggt ggagatgggg tcgagcgccg
      961 aacatggttc gtcgagcagc agcacttccg ggcgaatggc gataccacgc gcaatacaca
     1021 aacgctgttg ctgaccacca gagagagagt aaccgctctg gtgcaattta tctttggttt
     1081 cgttccacaa tgcggctttg gtcaatgccc actgcacgcg ctcgtccatg tcagcacggg
     1141 agagcttctc aaacagacgc acgccaaaag cgatgttgtc gtagatggac atcgggaacg
     1201 gcgtcggttt ctggaacacc atgcccactt tcgcacgcag cagggcgata tcctgagagt
     1261 tggtcaggat gttgtcgcca tcgagcagaa tttcaccttc cgcacgctgt tccgggtaca
     1321 gttcaaacat tttgttgaag gtacgcagca gcgttgattt accgcagccg gacggcccga
     1381 taaacgccgt aacctggttt ttggcgatat ccaggttgat gtttttcagg gcatggaatt
     1441 tgccgtagta gaagttcaaa ttacgaacct gaattttact cggggcagtt tcaaccatac
     1501 tcattgcaat cttttcctca tcgtgcctga tgcacttcgc gaatcaggct taccgtaggc
     1561 cggataaggc gtttacgccg catccagcaa agtgttaacc gtgtttattc ttcgcaaaaa
     1621 caacgcgcgc cagaatgttc agcagcagta cgcacagggt aatgatcaac accccggccc
     1681 aggccagttg ctgccattcc gcaaacgggc tcatcgcaaa cttaaagatc gtcaccggca
     1741 ggttggcgat cggctgcatc atgtccgtgc tccagaactg gttggagagc gcagtaaaca
     1801 gcagcggcgc ggtttcacca gcaatacggg caatcgccag caggataccg gtcatgatcc
     1861 cggacaccga cgctttcagc gtgatcgcgg caatcatctt ccacttcggc gtacccagcg
     1921 cataagccgc ttcacgcagg ctgtccggca ccagtttcag catgttctcg gtggtgcgga
     1981 taacaatcgg cacctgcaac agcgccagcg caatcacgcc cgcccagccg gagaagtgct
     2041 ccatctgcgc caccacgatg gtgtaaacaa aaagaccaac cacaatcgac ggtgcggaga
     2101 gcaaaatgtc gttaatgaag cgaatcactt ctgccagcca ggatttacga ccatattcag
     2161 ccagataaat ccccgccata atgcccagcg gcgtaccgaa taccgtggcc cacaaaatta
     2221 acaggccgct acccgccagg gcgttcgcca gaccaccgcc ttccgtattt ggcggcggcg
     2281 tcatttcggt gaacagcgcc agcgacatac catcgatacc gcgagtgatg gtggacatta
     2341 aaatccagat cagccagaac agaccgaagg ccatcgtcgc catcgagagc gtcagcgcaa
     2401 tacggttctt aaggcggcga cgcgcctgca ttttacggcg ggattcagcc agcgccgcag
     2461 tggtttgcat ttcaaccata gccattagcg tgccccctca ttcttagcca ggcgcataat
     2521 cataaacttc gatgcggcga ggacgatgaa ggtaatcaca aacaggatca ggcccagttc
     2581 catcagtgca gcaacatgca gacccgactc tgcttccgca aattcgttgg ccagcgcaga
     2641 ggtgatactg ttgcccggca tatacagcga ggcgctgtcg agttggtagg tgttaccgat
     2701 gataaaggtc accgccatgg tttcaccgag cgcgcgcccc agccccaaca tgatgccgcc
     2761 gataacacca tttttggtga acggaagaac gatacgccag ataacttccc aggtggtgca
     2821 gccaataccg taggccgact ctttcatcat caccggggtt tgttcgaata catcgcgcat
     2881 taccgccgca atgtacggaa taatcatgat ggcgaggatc acgcctgccg cgaggatacc
     2941 gataccaaag gccgggccgg agaacagcgc gccgacaatc gggatattcg acatgatatt
     3001 gccgaccggc tcctggaagt aaacggcgaa cagcggggcg aagataaaca agccccacat
     3061 gccgtaaacg atacttggaa tggcagccag cagctcaatg gcgataccca gcgggcgttt
     3121 cagccagcca ggcgcaagct cggtcaggaa cagggcgata ccaaaactca ccgggacggc
     3181 gatcagcagc gcgataaacg aggtcaccag cgtaccgtag atcggcacca gcgccccgta
     3241 gatatcgttc ggcgcatccc actctttggt ccacaggaaa gccagaccaa atttctgaat
     3301 gctcggccag gatgagatga tcagagagac aataatgcca cccaacatca atagcacagt
     3361 cagcgccgcc agttttacca gcacgctgaa aattatgtcg ccctttttac ccggtgggtt
     3421 aaaagcaggc ttggttgcag ccataagtta ctcttcagtt aaacgcgttt acaaagtcat
     3481 tttgtaatgc cggatgcggc gtaaaacacc gtacccggcc tggagtttta ttagtacagc
     3541 ggcttgccgc tactgtcttt aatattggtc ttccacgcag cgcgaacctg ttcaactacg
     3601 ctatccggca ggctggcata atccaggtcg ttcgcctgtt tagccccggt tttgtatgcc
     3661 cagtcgaaga acttcagcac ttcggtgcct tgttctggtt tcttctgatc tttatggatc
     3721 agaatgaacg tggtagaggt aataggccat gcatcttcgc ctttctggtt ggtcagatcc
     3781 tgagcgaatg ttttgctcca gtctgcgcct tttgctgcat tagcgaagtt ttcttcggtc
     3841 ggactcaccg gtttgccatc agcggagatc agtttggtgt acgccaggtt gttctgcttc
     3901 gcgtaagcat attcaacgta accaattgca cccggcagac gctgaacgaa cgcggcgata
     3961 ccgtcgttac ctttaccgcc cagaccgatc ggccatttta cggtagagcc agtaccaacg
     4021 ttgtttttcc actcttcgtt cactttcgcc aggtagctgg tgaagacgaa ggaagtcccg
     4081 gagccatctg cgcggcgtac tacagcaatg ttctgtgaag gcagtttcag acccggattc
     4141 agtttggcga tggcttcatc atcccacttc ttgattttgc ccaggtagat gtcgccgagg
     4201 gttttaccat ccagcaccag ttcgccagac ttcagccctg gaatgttaac cgccagcacc
     4261 acgccgccaa tcacggtcgg gaactggaac aggccttcct gagccagttt ttcgtcagac
     4321 agcggcgcgt cagaggcacc gaaatcaacg gtattagcga taatctgttt tacgccaccg
     4381 gaagaaccga taccctggta gttaacttta ttaccggttt ctttctggta agtgtcagcc
     4441 catttggcat acaccggcgc agggaaggtt gcacctgcac ctgtcaggct tgcttctgca
     4501 aacacagaga aagcactcat cgataaggtc gcggcgacaa cagttgcgac ggtggtacgc
     4561 ataactttca taatgtctcc tgggaggatt cataaagcat tgtttgttgg ctacgagaag
     4621 caaaatagga caaacaggtg acagttatat gtaaggttta tgacagtttt atgacaaatc
     4681 agaattatta attaactttt tgattttatt gactaagaaa aagaaaagta atgtcatctt
     4741 tttgtcatgc agagtgaaaa ctcgcggaca aaaatgcgta agatgacaac ggtgtgagga
     4801 acaccgctgt cagttcgttt agcgtacaat ctcaattgtt atcgttgcag tcgcggtaaa
     4861 tgttcctggc gcggggcgag cccccgtaag gcttgcagga gcagcagaaa aagtgacgct
     4921 accggctttt tcaccaaaga cggttgattt acccatatct gtcggcagtt caccaccgtt
     4981 aacattgatt tccttattat ttttatcata aattttaatc ccaacgttcg ggtttgatgt
     5041 cacgaccatt gagttgtcag tactgggttc ggtagcataa aaagaggtac tgatatcatc
     5101 agtatttagt attcctaaat tttcacactc aatggtgacg gttttggtaa ttttatgcgt
     5161 tgtcacggta cttccaacta ccgaagaaaa atctgccgca ggaatttctt taaaatcaaa
     5221 attaatgggt cttaaactgt taatttcaca gctttgcggt gcagtgatat cgccacgaat
     5281 ataaacatcc gccataggtt gtgagtaatc aaccggagtg tcacggctta tggttccgta
     5341 caatttcgcc acgagagttt caggaataat taactttcca gcaaaagtct tcttaatata
     5401 aaaataaatt attgcttttg caccactgga aatacttaat gggtccgtgc gactacattt
     5461 atatgaacct ccagtaggcc tgttagcatg gtattcaaaa ggtgcattaa tgtatcctac
     5521 accgagaagg tctatcgata ttccaacatc cagattagga ttcaaattaa agtagtttac
     5581 attagaacga acacctgcat aacctaagga ctcgtttatt ctcgctgtat aataaatata
     5641 aggaaactct cccacgttcc ctgtatcaca atggcagtga gcaggataag agtccccatt
     5701 tgtcacggtt ttattaaagg tctttcccac tctgttctcg tctgccgtaa tcagatagcc
     5761 ggaaaaatca agtgtgctat gaaaggaccc attatctgaa gtgcagatac cttttggggc
     5821 cgcaaatgtt gcgccactaa aggagaagat aaaaacaata aagcaccatg gtataatata
     5881 cttgttcata aaaatccttt taatgacatg tcacaccttg ttcaattaag ccgctttcac
     5941 tttctggtat tgaaagtgaa taatcgaagg tgcatgtagt ggcggcattt tctccccagg
     6001 agagcatcac ctgccctttt tcaggaaggc ccgagagata aagctccccc tggtcaccga
     6061 caattcctga atcagcggag cttgtatttt gtactgtagc aatagcgcca aaaggtgctg
     6121 gtttaccttt tgtacccgca accctgaaaa gcacacgata accaacacga gtaacaaatt
     6181 cggccttaac cactgcacca cgggtgggaa ccatcgtaac gacggtatta tcaagttcta
     6241 tattcgactt cgagaaatag ttatcgctga ttgctacccg gttttcatca taagggttta
     6301 gctgtgtctt gaccgtatag ccgcgccagt ccgtcgcagc accgcgcata ttgtctattt
     6361 ctaacccggc agcccctggt gctttaacaa gagctatggt ttcacccaac tcctggctta
     6421 acgttacgcc ttcgctgtgc gccagaattc cccctctgat accataattc agcctctgag
     6481 aattttggct gtagttatag ctgccgttca ccgtgccata agtgccatca taggtcaggg
     6541 acatattacc gccaacctca tcatcatggc tgttatagga ctgcattaaa ttccaggaaa
     6601 ggttatttct ttccagcgcc gtaccgctga gtccaagttg atgtgagcta ccggaattat
     6661 cgttttgtga aacagaatag gtggcgtaac tatttcccca ccagcccgat aaaggaacag
     6721 ataaagagag catgtagatc tgctcactgg tgttatcact ggcatggttg ttccccgtcc
     6781 cctgccgggc agtattggaa taactccagg agacgttcca cgagagacgc ttccagctac
     6841 tgctgtagcc aaattgcatt aatcgttcaa catcatcggt atgccagtac tgctggcgac
     6901 ttaatgtcag atataagttg ccatagtctc tgcccagtga ttgcgttaac gttccttcca
     6961 cacggctgcg gcggttaccg gtttcccaga aatcttcagg attattacgc cgcgacgccc
     7021 attcattgag agtatagtaa ccttcggtgg agtaacgata gccaaccagg cgcaagctgg
     7081 tatccgtatc gtcaaagcgt ttcgaataca gaaagcgata agactgaccg gtttccgtat
     7141 cagcatggct aaatttcgca cgggcatgtg tcacatcgaa agaaattgca cccagtgcgc
     7201 caagctcttt accgacaccc aaagcggcag aaccatactt ttcagcagcc tgcatacctc
     7261 catacaaggt gaaaccgtag ggaaaaccat gaaggatctg cacctgaaga acgtcagggg
     7321 taaagccatc ttcatcgcgt aattctccca cactgacatc aacatcagtt tgaccttcac
     7381 gtttgaggat ggctaacgac gcataaggtt gagtaaatcg ttgttcgcta ccgtcttctt
     7441 ctttgatggt tacgtccaga tcaccacctg tactggcggt gttgaggtcg gtaatttcaa
     7501 aagcaccttg tggaacggct gactgataaa tcacgtagcc attctggcga ataatgacag
     7561 tggcgttaga ctttgcaatc ccacgcacta cgggggcaaa tccgttttgg ctggcaggca
     7621 acatatcgtt atcggtatac aagcgtgcgc cacgaatttg cgtactgtcg aagatatcgc
     7681 tcgccgtcca cgtatcacct atcataatct ggcttcttaa cgcagctata tcacgttgta
     7741 tccaggtgct tatagagttg tattctgcac tgccatcggt cgtatttaag gtgctgtaat
     7801 tgcgtaatcg ccacggccca atattcagtc cattgcgcag attgagataa ctggtatctg
     7861 tctcttgtga accataatcc gaacgtagag tatgtgaacc agaaaaatcg tagttgatgg
     7921 tgagcgcgtt aatcccctca tcccaacgcg aggggctgat gtaatcataa gccatcgcat
     7981 ccatatagat ttggggaatg gagatattca gttgcagttt atcgctgtcg aactgccatg
     8041 atgagccttc aatgcgtggc ccaggatcaa tacagactgt ttcatctaca tcattgagcg
     8101 atttcagcgc atcaactttt acgccaaagg tggttagcgt ttttttaata gcgacaaagc
     8161 aggcgtgtaa atcagcatcg ggattatcct gaacaaaggt cacatcctga cgttcaaaca
     8221 ttttaccgtt gataaaaata tcaacgctat aaacaccagg gggcatcagg tttgcccgat
     8281 taaactggcg aatatcgact ccggattgat ggtcgatttc cagaagcgcc ggattgaata
     8341 cactgacctc ggccatcagg ctttgcgaga aaatcagaca cgtccctgcc gtgaggccaa
     8401 ccactattct ggtcgtcatc ataatacgcc tatccttatc ccttgcttat agcgtcattt
     8461 ttatcgggtc actgactgcg ccgtaatcat taataaatcg ccaggtcatt tgacctcccg
     8521 cagaattagc aggtaaaggt aacgtgagcg tttcaaaagg acggatcatg ttgaggggca
     8581 catcatgacc atcaacgatg acatccatca cactgatatg aatcgggttg ggattctctg
     8641 caatcaggtt tcgcccttcc cggtgccact tcaccttcgg tgcagcatct tcgggaatca
     8701 ggcgaatgct tgccggtcgc cagaacagct tgatacgcgt tttaactgcg atttccagtg
     8761 agttcgccgc tttgttgctc gcagagggcg gaatattttt aatattcagc cagaagactg
     8821 actcgcgatc atcaggcaat gacacacctt tggtatgaat aatgcgtaat actttttctt
     8881 gcgcgggttc taatcgtgat accggaggaa taacggtaaa cggtgctttg cttttatcct
     8941 ctttattaaa cggatctacc catgcctgga tgagataagg aattttttct tccttgctaa
     9001 atacagaaat agatttatct gggttattac ttaagtaaac tacccgtgta ccaccaatga
     9061 caacgcctgc ataggttaat gctggttgga ctaaaaaaac actgagtata atcgcggtaa
     9121 ataacttaca tgaaacggtt gttttaaaga gcctgattaa acaacttttt ctggacataa
     9181 cgactccctg gccaaagcca actgaataaa aaggcccttg ataaattatt tcttatattc
     9241 gacagtaaat tgtgaatcgg cgttcgccgt acccgccgtt acggttgcat tggttgccac
     9301 atagcgtgca ataaacttca tttcagcctt gccttcagcg tcgatagcgg tataggcaga
     9361 atcgttataa agtttaacct gcgagttatc tgactggtta aaaatacgaa taccgacacc
     9421 cgttgccgca atcgcttgtc ctgtaccggt aaaatcgcca gggttgcccg ctgtaagcgg
     9481 cgtacccact ttcaggtcgc cgtcgccgcc aggcgcttct gtaccatcaa aacgaacggc
     9541 tgcctgagtg taggttgccg ggcacttttc aagtttaata ctgaactgct gtggtgacgc
     9601 cagactacct acacctgaaa atgcattttt actgacttta cccagtggga cggtttgatc
     9661 ggctgtatcg gaggtaactt cacaagtcgt gtcgacaatt tcaccgacga agtgaacaac
     9721 accatcttct gccagtgcat gtccggccag cattaaagat gccagagtga atgcaccgcc
     9781 tataatatta cgcttcat
//
LOCUS       DAZFLD010000175         9718 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.176, whole genome shotgun sequence.
ACCESSION   DAZFLD010000175 DAZFLD010000000
VERSION     DAZFLD010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9718
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.176"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            56..1309
                     /gene="ynfM"
                     /locus_tag="ACHKBL_003779"
     CDS             56..1309
                     /gene="ynfM"
                     /locus_tag="ACHKBL_003779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO5820585.1"
                     /translation="MSRTTTVDGAPASDTDKQSISQPNQFIKRGTPQFMRVTLALFSA
                     GLATFALLYCVQPILPVLSQEFGLTPANSSISLSISTAMLAIGLLFTGPLSDAIGRKP
                     VMVTALLLASICTLLSTMMTSWHGILIMRALIGLSLSGVAAVGMTYLSEEIHPSFVAF
                     SMGLYISGNSIGGMSGRLISGVFTDFFNWRIALAAIGCFALASALMFWKILPESRHFR
                     PTSLRPKTLFINFRLHWRDRGLPLLFAEGFLLMGSFVTLFNYVGYRLMLSPWHVSQAV
                     VGLLSLAYLTGTWSSPKAGTMTTRYGRGPVMLFSTGVMLFGLLMTLFSSLWLIFAGML
                     LFSAGFFAAHSVASSWIGPRAKRAKGQASSLYLFSYYLGSSIAGTLGGVFWHNYGWNG
                     VGAFIALMLVIALLVGTRLHHRLHA"
     gene            complement(1359..1478)
                     /locus_tag="ACHKBL_003780"
     CDS             complement(1359..1478)
                     /locus_tag="ACHKBL_003780"
                     /inference="COORDINATES: protein motif:HMM:NF033695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KPN_01571 family protein"
                     /protein_id="HFO5820586.1"
                     /translation="MTHSKMNPFIWVLIILMTLDALRELAGASSILGWLLTLV"
     gene            1734..2042
                     /gene="asr"
                     /locus_tag="ACHKBL_003781"
     CDS             1734..2042
                     /gene="asr"
                     /locus_tag="ACHKBL_003781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance repetitive basic protein Asr"
                     /protein_id="HFO5820587.1"
                     /translation="MKKVLALVVAAAMGLSSAAFAAETATTPAPTATTTKAAPAKTTH
                     HKKQHKAAPAQKAQAAKKHHKNAKAEQKAPEQKAQAAKKHAKKHSHQQPAKPAAQPAA
                     "
     gene            2135..2218
                     /locus_tag="ACHKBL_003782"
     CDS             2135..2218
                     /locus_tag="ACHKBL_003782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5820588.1"
                     /translation="MVGRYRFEFILIILILCALIAARFYLS"
     gene            2318..3139
                     /gene="ydgD"
                     /locus_tag="ACHKBL_003783"
     CDS             2318..3139
                     /gene="ydgD"
                     /locus_tag="ACHKBL_003783"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HFO5820589.1"
                     /translation="MRRTIAVVLGAISLTSAFVFADKPDVAKSANDEVSTLFFGHDDR
                     VPVNDTTQSPWDAVGQLETASGNLCTATLIAPNLALTAGHCLLTPPKGKADKAVALRF
                     VSNKGLWRYEIHDIEGRVDPTLGKRLKADGDGWIVPPAAAPWDFGLIVLRNPPSGITP
                     LPLFEGDKAALTAALKAAGRKVTQAGYPEDHLDTLYSHQNCEVTGWAQTSVMSHQCDT
                     LPGDSGSPLMLHTDDGWQLIGVQSSAPAAKDRWRADNRAISVTGFRDKLDQLSQK"
     gene            complement(3178..3507)
                     /gene="mdtI"
                     /locus_tag="ACHKBL_003784"
     CDS             complement(3178..3507)
                     /gene="mdtI"
                     /locus_tag="ACHKBL_003784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtI"
                     /protein_id="HFO5820590.1"
                     /translation="MAQFEWVHAAWLALAIVLEIVANVFLKFSDGFRRKIFGLLSLAA
                     VLAAFSALSQAVKGIDLSVAYALWGGFGIAATLAAGWILFGQRLNRKGWIGLVLLLAG
                     MIMVKLA"
     gene            complement(3494..3859)
                     /gene="mdtJ"
                     /locus_tag="ACHKBL_003785"
     CDS             complement(3494..3859)
                     /gene="mdtJ"
                     /locus_tag="ACHKBL_003785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015606 - spermidine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015848 - spermidine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtJ"
                     /protein_id="HFO5820591.1"
                     /translation="MYIYWILLGLAIATEITGTLSMKWASVSEGNGGFILMLVMISLS
                     YIFLSFAVKKIALGVAYALWEGIGILFITLFSVLLFDESLSLMKIAGLTTLVAGIVLI
                     KSGTRKARKPELEVNHGAV"
     gene            4270..5304
                     /gene="tqsA"
                     /locus_tag="ACHKBL_003786"
     CDS             4270..5304
                     /gene="tqsA"
                     /locus_tag="ACHKBL_003786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
                     /protein_id="HFO5820592.1"
                     /translation="MAKPIITLNGLKIVIMLGMLVIILCGIRFAAEIIVPFILALFIA
                     VILNPLVQHMVRWRVPRVLAVSILMTIIVMAMVLLLAYLGSALNELTRTLQQYRNSIM
                     TPLKAIEPLLQRVGIDVSVDQLAHYIDPNAAMTLLTNLLTQLSNAMSSIFLLLLTVLF
                     MLLEVPQLPGKFQQMMARPVEGMAAIQRAIDSVSHYLVLKTAISIITGLVAWAMLAAL
                     DVRFAFVWGLLAFALNYIPNIGSVLAAIPPIAQVLVFNGFYEALLVLAGYLLINLVFG
                     NILEPRIMGRGLGLSTLVVFLSLIFWGWLLGPVGMLLSVPLTIIVKIALEQTAGGQSI
                     AVLLSDLNKE"
     gene            complement(5329..6717)
                     /gene="pntB"
                     /locus_tag="ACHKBL_003787"
     CDS             complement(5329..6717)
                     /gene="pntB"
                     /locus_tag="ACHKBL_003787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HFO5820593.1"
                     /translation="MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIA
                     LIATILGPDTGNVGWILLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYLHHDAGMAPILVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNR
                     HKMNLAALVVSFLLLIVFVRTDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVV
                     SMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFG
                     TDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQAQYPVAEITEKLR
                     ARGINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGAND
                     TVNPAAQDDPKSPIAGMPVLEVWKAQNVIVFKRSMNTGYAGVQNPLFFKENTHMLFGD
                     AKASVDAILKAL"
     gene            complement(6728..8260)
                     /gene="pntA"
                     /locus_tag="ACHKBL_003788"
     CDS             complement(6728..8260)
                     /gene="pntA"
                     /locus_tag="ACHKBL_003788"
                     /EC_number="7.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HFO5820594.1"
                     /translation="MRIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLAS
                     FDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELM
                     QKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
                     GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKE
                     EAGSGDGYAKVMSDAFIKAEMELFTAQAKEVDIIVTTALIPGKPAPKLITREMVDSMK
                     AGSVIVDLAAQNGGNCEYTVPGEIFTTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNL
                     LKLLCKEKDGNITVDFDDVVIRGVTVIRAGEITWPAPPIQVSAQPQAAQKAAPEVKTE
                     EKCACSPWRKYALMALAIILFGWLASVAPKEFLGHFTVFALACVVGYYVVWNVSHALH
                     TPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFR
                     KN"
     gene            8784..>9718
                     /gene="ydgH"
                     /locus_tag="ACHKBL_003789"
     CDS             8784..>9718
                     /gene="ydgH"
                     /locus_tag="ACHKBL_003789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YdgH"
                     /protein_id="HFO5820595.1"
                     /translation="MKLKNTLLASALLSATAFSVNAATELTPEQAAAVKPFDRVVVTG
                     RFNAIGEAVKAVSRRADKEGAASFYVVDTSDFGNSGNWRVVADLYKADAEKAEETSNR
                     VINGVVELPKDQAVLIEPFDTVTVQGFYRSQPEVNDAITKAAKAKGAYSFYIVRQIDA
                     NQGGNQRITAFIYKKDAKKRIVQSPDVIPADSEAGRAALAAGGEAAKKVEIPGVATTA
                     SPSSEVGRFFETQSSKGGRYTVTLPDGTKVEELNKATAAMMVPFDSIKFSGNYGNMTE
                     VSYQVAKRAAKKGAKYYHITRQWQERGNNLTVSADL"
BASE COUNT         2439 a         2514 c         2328 g         2437 t
ORIGIN      
        1 ttttcctaat ctattcataa gcctgaacca caggcatatt tagcaaggat ttcaagtgag
       61 ccgtactaca actgttgatg gcgctccggc aagcgacact gacaagcaaa gcatttctca
      121 gccaaatcaa tttattaaac gcggtacgcc gcaatttatg cgcgtcaccc tggcgctgtt
      181 ctctgccgga ctggcaacct tcgcacttct ctattgtgtg caacctatcc ttccggtgct
      241 ttcgcaggag tttggcttaa cgcccgcgaa cagtagtatt tcactgtcca tttctacggc
      301 gatgttggct atcggtttgc tgtttactgg cccgttatcc gatgccattg gtcgcaaacc
      361 agtgatggtc acggcgctac tgttggcctc catttgtacg ttactttcga caatgatgac
      421 cagctggcac ggcattttga ttatgcgcgc cttgattggg ctttcgttaa gtggcgtagc
      481 agctgttggc atgacttatc ttagcgagga aatccacccc agtttcgtgg ccttttcgat
      541 ggggttgtat atcagcggca actcaattgg cggcatgagc ggacgcttaa ttagcggtgt
      601 cttcacggac tttttcaact ggcgaattgc gctggcggca atcggttgtt tcgcgctggc
      661 ctcggcgtta atgttctgga aaatcctccc tgaatcacgc cattttcgcc cgacttcact
      721 gcgccctaag acgttattca tcaactttcg tctgcactgg cgtgaccggg gattaccgtt
      781 attgttcgca gaaggctttt tgctgatggg gtcgttcgtc acgctgttta attacgtcgg
      841 ctatcggttg atgctctccc cctggcatgt cagccaggcc gtggttggct tattatcgct
      901 ggcttatttg accggtacat ggagctcacc caaagccgga accatgacca cccgctatgg
      961 gcgcggtccg gtgatgttgt tttcgacggg ggttatgctg tttggtttat tgatgacctt
     1021 attcagctcg ctgtggctga tctttgccgg aatgttactc ttctcagccg gattcttcgc
     1081 agcccactca gtagccagca gctggatcgg cccccgcgca aaacgcgcta aaggccaggc
     1141 ctcctcgctg tatctgttca gttactatct ggggtcgagt attgccggga cgctgggtgg
     1201 tgttttctgg cataactatg gctggaacgg cgtcggcgca tttattgctc tgatgctggt
     1261 cattgctctg ctggtcggga cgcgtttgca tcatcgtctg cacgcctgaa aaaataagtc
     1321 cggactgagg taaatacccg tccggactta ttgcctgctc aaaccaacgt taatagccat
     1381 cctaaaatag acgaagcgcc agccaattcc cgcagcgcat ctagtgtcat caggattata
     1441 agtacccaaa taaacggatt cattttgctg tgtgtcattt attactgatg cgcagttatt
     1501 ctaccgcttt gtaagtagta aaatagttaa cacgatcaag actactatta ttggtagatg
     1561 aattttcctt aagtcacaat acgttattat caacgctgta atttattcag cgtttgtaca
     1621 tatcgttaca cgctgaaacc aaccactcac ggaagtctgc cattctcagg gatatagtta
     1681 tttcaacggc cccgcagtgg ggttaaatga aaaaacaaat tgagggtatg acaatgaaaa
     1741 aagtattagc tctggttgtt gccgctgcta tgggtctgtc ttctgctgcc tttgctgcag
     1801 agactgcgac cacacctgct ccgactgcga cgaccaccaa agcagcgcct gcgaaaacca
     1861 cacatcataa aaaacagcat aaagcagcac ctgcccagaa agcgcaggcg gctaaaaagc
     1921 atcataaaaa tgccaaagct gaacagaaag ctcctgaaca aaaagcgcag gcagcgaaga
     1981 aacacgccaa gaaacacagc catcagcaac cggcaaaacc tgctgcacaa cccgcagcgt
     2041 aagttttcaa cagtaatgct ggcgcgcccc ctcgcgcctg aaaattacgg tgctaaacgg
     2101 gtaacgttta gcaccgcctt tagcggaggg tagtatggtg ggccgttatc gctttgagtt
     2161 cattcttatc atccttattt tatgcgcact gattgccgcc cgtttttatc tttcctgatc
     2221 gtagttatct gattttactc ccactttcat cccgtcccgt ctatagtatt tatgagggtt
     2281 tgcttttaat aatcataatt acccaccaga gtgtgatatg cgtagaacca ttgctgtagt
     2341 gttgggtgca attagtttga cgtctgcttt tgtgtttgca gataaaccag acgttgccaa
     2401 atcggcaaac gatgaggtca gcaccctgtt ttttggtcat gatgatcgtg tgccagtgaa
     2461 tgacacgacc caatcaccgt gggatgcggt tggccaactg gaaacggcca gcggcaattt
     2521 atgtacagcg acgctgattg cacccaatct ggcattaacg gcaggacact gtttattgac
     2581 gccgccaaag ggtaaagccg ataaagcggt ggcgctgcgt tttgtgtcaa ataaaggtct
     2641 ttggcgctat gagatccacg acatagaagg ccgcgttgac ccgacactgg gaaagcggtt
     2701 aaaagcagat ggggatggtt ggattgttcc tcccgcagct gcgccgtggg acttcggttt
     2761 gattgtgcta cgtaatcccc cttctggcat tacaccattg ccgttatttg agggagataa
     2821 agccgcgctt accgccgcat taaaagcggc aggtcgtaaa gtgactcagg cgggataccc
     2881 tgaagatcat ctcgatacgt tgtacagtca tcaaaactgt gaagtgactg gctgggcgca
     2941 aacgtcggtg atgtcgcatc agtgcgatac cttgccgggt gacagcggtt cgcctctgat
     3001 gttgcatacc gatgacggct ggcaattaat tggggtgcaa agttcggctc ctgccgcgaa
     3061 agatcgctgg cgcgccgata accgggccat ttctgttacc ggttttcgcg acaagctgga
     3121 tcaactgtcg caaaaataat gttcaaacgc tgcccgacag cgcgggcagc atgttcatca
     3181 ggcaagtttc accatgatca ttccagccaa cagcagcacc aggccaatcc agcctttacg
     3241 atttaaccgt tgaccaaaca agatccaacc tgcggctaac gtggcggcaa taccaaaccc
     3301 gccccacaat gcataagcga cagacaagtc gatcccttta acggcctgag aaagcgcact
     3361 aaaggcagcc agcaccgccg ccagagagag caagccaaat attttgcgac gaaagccgtc
     3421 agaaaatttc aaaaagacgt tagcaacgat ttccagcacg attgccaatg ccagccaggc
     3481 ggcgtgaacc cattcaaact gcgccatggt tcacctccag ttcaggttta cgcgctttac
     3541 gggtacctga tttgatcaac acaatcccgg cgaccagggt ggttaacccg gcaattttca
     3601 tcagagataa actttcgtcg aataacaaaa cgctaaacaa ggtaataaat aaaataccga
     3661 taccttccca cagcgcataa gctacgccta aggcgatctt tttaacggcg aaagagagaa
     3721 atatatacga cagagaaatc atcaccagca ttaaaataaa gccgccattt ccctcactga
     3781 cgctcgccca tttcatggac agcgtaccgg taatttctgt agcaatagcc agacctaata
     3841 aaatccaata aatatacatt gtccttctcc tgcaagagaa ttattttaat tttcgcttaa
     3901 ttcagcgaaa ccaaagtaaa gtggtcaagc ccgcgcttag cgcaagtttt ggcagaagaa
     3961 aggactaaag cgcgttgcgc cagtgctgct cacctacgag caagatagtg gatgaggtac
     4021 gaagatgggt aaatgtagaa aataacgtcc tgaacaaatt gtccataata ttacaattat
     4081 ccgcagtgtt gcttctcgtc atcgcggatg ataattgtcc tcggtagttg aacacgcctg
     4141 atttgtatca tagcttaaga attaactcaa aatattttca cttctttacc tgagcggttt
     4201 gattttcgtt atgatgacgg agcgaaaaag acattattat tagcaaagga agaaaaaacg
     4261 gggacaagca tggcaaagcc gatcatcacg ctcaatggcc taaaaatcgt cattatgttg
     4321 ggaatgctgg tcattattct ctgcggtatc cgttttgccg ccgagatcat cgtgccgttt
     4381 attctcgcat tatttattgc tgttattctt aacccgctgg tgcaacacat ggtccgctgg
     4441 cgtgtgccgc gtgtactggc agtgtcaatt ttgatgacca tcatcgtgat ggcgatggtg
     4501 ttgctgttag cttatctggg ttccgcgctc aatgagttga cgcggacgtt acagcaatat
     4561 cgcaactcta ttatgacgcc actgaaagcg attgaaccgt tgttgcaacg cgtagggatt
     4621 gatgtctcag ttgaccaact ggcgcattac attgatccga acgcggcgat gacgttgctc
     4681 accaacttat tgacgcagtt atctaatgcc atgtcatcaa tctttttatt gctgctgacg
     4741 gtgctgttta tgctgctcga agtgccacaa ttgcccggaa aatttcagca aatgatggcg
     4801 cgtccggttg aagggatggc ggcgattcag cgtgcgattg acagtgtgtc tcattatctg
     4861 gtgctgaaaa cagccatcag catcatcacc ggcctggtcg catgggcgat gctcgccgca
     4921 ctcgatgttc gcttcgcttt tgtctgggga ttgctggcct ttgcgcttaa ttacatcccg
     4981 aatattggtt cagttctcgc ggcaatcccc cctattgctc aggtactggt gtttaatggc
     5041 ttctacgaag cgttgctggt gctggcggga tatctgctga ttaatctggt cttcggcaat
     5101 attctggagc cgcgcatcat gggacgtggg ctggggcttt cgacattggt ggtatttttg
     5161 tcgttgattt tttggggatg gttgttagga ccggtgggta tgctgctttc cgtgccgttg
     5221 acaattattg tcaaaattgc gcttgaacaa acagcgggag gtcaaagcat cgccgttctg
     5281 ttaagcgatc tcaataaaga gtgacggcct ccgcagaggc cgtcagggtt acagagcttt
     5341 caggattgca tccacgctgg ctttggcgtc accaaacagc atgtgggtgt tttccttgaa
     5401 gaacagcggg ttttgcacac cagcatagcc agtgttcatc gaacgtttaa agacaatcac
     5461 gttctgcgct ttccacactt ccagcacagg cataccagca ataggactct tcggatcatc
     5521 ctgcgccgcc gggttaaccg tatcgttagc accaatcacc agtacggtat cggtatcagc
     5581 aaagtcatcg ttgatctcgt ccatttccag cacgatgtca tacggtactt ttgcttcagc
     5641 cagcaatacg ttcatatgtc caggcaaacg ccccgcaacc gggtggatac cgaaacgcac
     5701 gttgatacca cgagcgcgca gtttctcggt aatttcagcg acaggatatt gcgcctgcgc
     5761 gactgccatg ccgtaccccg gagtaatgat cactgaatgg gagtttttca gcagttccgc
     5821 tgtctcttct gcggtgattt cgcggtgctc gcccacttcc tgatcatcgc cagtagaaga
     5881 gccgtcggtg ccgaaaccac ccgcaataac gctgataaag gaacggttca tcgccttaca
     5941 cataatgtaa gaaaggatag cgcccgaaga accgaccagc gcaccggtca caatcagcag
     6001 atcgttgctg agcataaagc ccgcagccgc agccgcccag ccggagtacg agttcagcat
     6061 cgacaccacc actggcatat ctgcaccacc gatggaggcg actaaatgcc agccgaatac
     6121 cagcgcaatg gcggtcatta tcagcaatgc cagcacttgc aggccgacgc tgtccgtgcg
     6181 aacaaataca atcagcagca ggaaggaaac gaccagagcc gccaggttca ttttgtgacg
     6241 gtttggcagc atcaacggtt tagacgaaat cttgccacac agtttgccga acgccaccac
     6301 cgaacccgtg aacgttaccg ccccgatgaa gataccgagg aacacttccg tcaggtgaat
     6361 attgaccaga atcggtgcca ttcccgcgtc atgatgcaga tagctgttaa agccaaccag
     6421 cactgccgcc agacccacga agctgtgcag gatcgccacc agttctggca tttcggtcat
     6481 ttcaactttc ttcgccagcc ggataccaat tgccccacca atgaccatcg ccagcaagat
     6541 ccagccaaca ttacccgtat ccggtcccaa aatggttgcg attaacgcaa tcgccatccc
     6601 ggcgataccg aagttgttac cctggcgaga cgtttcatgt ttcgaaagac cggccagact
     6661 gaagataaac aggatcgcgg caacaatgta tgcagctgta actaatcctc cagacatatg
     6721 ttacccctta atttttgcgg aacattttca gcatgcgctg agtcacggtg aagccaccga
     6781 aaatattaat gctggctata agcaccgcga taaaactaag gaagctaacc cagccgccct
     6841 ggccaatctg caacagtgct ccgacaacaa taatccctga aatcgcgttg gtgaccgaca
     6901 tcaacggtgt atgcagcgcg tgcgatacat tccacaccac gtaataaccg acaacacagg
     6961 ccagcgcaaa aacggtgaag tgcccaagaa actctttcgg cgcaacgctt gccaaccagc
     7021 cgaaaagaat gattgccagc gccatcaacg cgtatttacg ccacggtgag caggcacatt
     7081 tttcctcggt tttcacttcc ggtgccgctt tttgtgccgc ctgcggctga gctgatacct
     7141 gaatcggcgg tgccggccag gtaatttcgc ccgcacggat cacggtcacg ccgcgaatca
     7201 ccacatcatc aaaatcaaca gtgatattgc cgtctttctc tttgcacaac agtttcagca
     7261 gattaacgag gttagtaccg taaagctgtg aggattgcgt cggcagacgg cccggaagat
     7321 cggtataacc aatcactttg acaccatttt ccgtagtgaa gatttcaccc ggcacggtgt
     7381 attcacagtt gccgccgttt tgggctgcca ggtcgacaat cacactaccc gccttcatgg
     7441 agtcaaccat ttcacgggta attagcttcg gcgctggttt gcctggaata agcgcggtgg
     7501 tgacaatgat atcgacctct tttgcctggg cggtaaagag ttccatttcc gctttgatga
     7561 acgcgtccga catcactttg gcatagccat cgccgctgcc cgcttcctct ttaaaatcca
     7621 gctcgaggaa ttccgcgccc atactttgaa cttgttcttt cacttccggg cgggtgtcga
     7681 atgcacgcac aatcgcgccg agactgtttg cagcgccaat ggcggccaga cccgcaacac
     7741 ccgcaccaat caccatcact tttgccggtg gcactttccc ggccgcagta atttgcccgg
     7801 taaagaagcg cccaaattca tgtgccgctt caacaatggc gcgataaccg gcgatattcg
     7861 ccatcgagct tagtgcgtcc agcgattgtg cgcgtgagat acgcggcaca gagtccatcg
     7921 ccatcacggt cacgttacgt tccgcaagtt tttgcattaa ttccggattc tgcgcaggcc
     7981 agataaaact caccagcgtt gtcccaggat tcagtaacgc aatttcatca tctaacggcg
     8041 cattgacctt cagaatgatc tctgactgcc agacgctatt cccttctaca atttcagcgc
     8101 ccgcttgcac aaacgcttta tcgtcaaaac ttgccagttg acccgcgccg ctctctaccg
     8161 cgacggtaaa acccagtttc agcagctgtt ccactgtttt tggcgttgct gcaacacggg
     8221 tttcattggt taaccgttct cttggtatgc caattcgcat gatattccct tccatcggtt
     8281 ttattgatga tggtttgcct gtgtcaggag ccacacaagc cgctcatgta cgagctaaat
     8341 gttactccgt taaaataaat tagtaacaaa cgccctataa cgtactgaaa attatgcctg
     8401 tgatctagcg ccaaaaatca gtatttcggc gtgaactcgc aaaatattaa cgattcagcc
     8461 gtgatagtgg gataaacacc ttagaacgcc ggataaagac tgataattgt cttcggcgtt
     8521 cgcataaaac gagacatcgc cccggcacga atcactactt aacattaaat taacttatac
     8581 aattcagttg cttcagtagt attgatgctg atacggctgt tttttaagca tagacggcca
     8641 tttgagcagg attaaaattg gcttaaggaa tgtgatatga aaaatgacgc agacagttac
     8701 accgtttaaa tgcaataatc agccacgttt ctcgttaata acaataccag tacctggttt
     8761 gcgcaaggcg aaggattatt tttatgaagc ttaagaacac cctcctggcg tcggcactgc
     8821 tttctgctac ggcattctcc gttaacgcag caacagaact gacaccggag caagcggcag
     8881 cggttaaacc ttttgaccgt gtagtggtta ccggtcgttt taatgctatt ggcgaagcgg
     8941 tgaaagccgt ttctcgtcgc gctgataaag aaggtgccgc ctctttttat gttgtcgaca
     9001 cttctgattt tggtaacagc ggtaactggc gtgtggtcgc tgacctctat aaagccgatg
     9061 ctgaaaaagc agaagaaaca agtaatcgcg taattaacgg tgttgtcgaa ctgccgaaag
     9121 atcaggctgt tctgattgaa ccgtttgaca cggtcaccgt ccagggcttc tatcgtagcc
     9181 agccagaagt caatgatgcc atcaccaaag cggcaaaagc gaaaggtgcc tactctttct
     9241 acatcgttcg tcaaatcgat gccaaccagg gcggcaacca gcgtattact gcattcatct
     9301 ataaaaaaga tgctaagaaa cgtatcgtcc agagcccgga tgtgatcccg gcagattccg
     9361 aagcaggacg tgcagctctg gctgccggtg gcgaagctgc gaagaaagtt gagatcccgg
     9421 gtgttgcgac taccgcatca ccaagttctg aagtcggtcg cttctttgaa acccagtcat
     9481 caaaaggcgg gcgttacact gtcacgctcc cagacggcac taaagtcgaa gaactgaaca
     9541 aagcgaccgc agcgatgatg gtcccgttcg acagcatcaa attctctggc aactacggca
     9601 acatgactga agtctcctat caggttgcga aacgtgccgc gaagaaaggt gctaagtatt
     9661 accacatcac ccgccagtgg caagaacgtg gtaataacct gaccgtcagc gcagatct
//
LOCUS       DAZFLD010000176         9599 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.177, whole genome shotgun sequence.
ACCESSION   DAZFLD010000176 DAZFLD010000000
VERSION     DAZFLD010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9599
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.177"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(17..595)
                     /gene="mocA"
                     /locus_tag="ACHKBL_003790"
     CDS             complement(17..595)
                     /gene="mocA"
                     /locus_tag="ACHKBL_003790"
                     /EC_number="2.7.7.76"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070567 - cytidylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor cytidylyltransferase"
                     /protein_id="HFO5820596.1"
                     /translation="MSAIDCIITAAGLSSRMGQWKIMLPWQQGTILDTSIKNALQFCS
                     RIILVTGYRGNELHERYANQSNITIIHNPDYAQGLLTSVKAAVPAVQTEHCFLTHGDM
                     PTLTIDIFRKIWSLRNDGAILPLHNGIPGHPILVSKPCLMQAIQRPNVTNMRQALLMG
                     EHYSVEIENAEIILDIDTPDDFITAKKRYTEI"
     gene            699..1469
                     /gene="yqeC"
                     /locus_tag="ACHKBL_003791"
     CDS             699..1469
                     /gene="yqeC"
                     /locus_tag="ACHKBL_003791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium cofactor biosynthesis protein YqeC"
                     /protein_id="HFO5820597.1"
                     /translation="MKSIIDPSALFIDLGAQKRPTVISVVGAGGKTSLLFWLAELFQA
                     SGRRVLITTTTHMFMPTSHWPVVFCRDPAMLPHASFISPISFCFHSWKANQGKVQGFT
                     PEAIDALVQRSECDVILIEADGSRGMPLKAPDEHEPCIPKSSCCVIAVMGGHILGAKV
                     STENVHRWSQFADITGLTPDATLQLSDLVALVRHPQGAFKNVPQGCRRVWFINRFSQC
                     ENAIAQSELLQPLQQHDVEAIWLGDIQEHPAIARRFVN"
     gene            1517..3142
                     /gene="yqeB"
                     /locus_tag="ACHKBL_003792"
     CDS             1517..3142
                     /gene="yqeB"
                     /locus_tag="ACHKBL_003792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-dependent molybdenum cofactor
                     biosynthesis protein YqeB"
                     /protein_id="HFO5820598.1"
                     /translation="MNIFTEAAKLEEQNCPFAMAQIVDSRGSTPRHSAQMLVRADGSI
                     VGTIGGGMVERKVIEESLQALQERKPRLFHGRMARNGADAVGSDCGGAMSVFISVHGM
                     RPRLVLIGAGHVNRAIAQSAALLGFDIAVADIYRESLNPELFPPSTTLLHAESFGAAV
                     EALDIRPDNFVLIATNNQDREALDKLIEQPIAWLGLLASRRKVQLFLRQLREKGVAEE
                     HIARLHAPVGYNIGAETPQEIAISVLAEILQVKNNAPGGLMMKPSHPSGHQLVVIRGA
                     GDIASGVALRLYHAGFKVIMLEVEKPTVIRCTVAFAQAVFDGEMTVEGVTARLATSSA
                     EAMKLTECGFIPVIVDPACSLLDELKPLCVVDAILAKQNLGTRADMAPVTIALGPGFT
                     AGKDCHAVIETNRGHWLGQVIYSGCAQENTGVPGNIMGHTTRRVIRAPAAGIMRSNVK
                     LGDLVKEGDVIAWIGEHEIKAPLTGMVRGLLNDGLAVVGGFKIGDIDPRGETADFTSV
                     SDKARAIGGGVLEALMMLMHQGVKATKEVLEVA"
     gene            complement(3183..4115)
                     /gene="arcC"
                     /locus_tag="ACHKBL_003793"
     CDS             complement(3183..4115)
                     /gene="arcC"
                     /locus_tag="ACHKBL_003793"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFO5820599.1"
                     /translation="MSKKIVLALGGNALGDDLAGQMKAVKITSQAIVDLIAQGHEVIV
                     THGNGPQVGMINQAFEAAAKTEAHSPMLPMSVCVALSQGYIGYDLQNALREELLSRGI
                     NKPVATLVTQVEVDANDPAFLNPTKPIGSFFTEQEAEQLTKQGYTLKEDAGRGYRRVV
                     ASPKPVDIIEKETVKALVEAGQVVITVGGGGIPVIREGNHLRGASAVIDKDWASARLA
                     EMIDADMLIILTAVEKVAINFGKENEQWLDRLSLSDAERFIEEGHFAKGSMLPKVEAA
                     ASFARSRAGREALITVLSKAKEGIEGKTGTVICQ"
     gene            complement(4163..5548)
                     /gene="hyuA"
                     /locus_tag="ACHKBL_003794"
     CDS             complement(4163..5548)
                     /gene="hyuA"
                     /locus_tag="ACHKBL_003794"
                     /EC_number="3.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-phenylhydantoinase"
                     /protein_id="HFO5820600.1"
                     /translation="MRVLIKNGTVVNADGQAKQDLLIESGIVRQLGSNISPQVPYEEI
                     DATGCYVFPGGVDVHTHFNIDVGIARSCDDFFTGTRAAACGGTTTIIDHMGFGPNGCR
                     LRHQLEVYRGYAAHKAVIDYSFHGVIQHINHAILDEIPMMVEEGLSSFKLYLTYQYKL
                     NDDEVLQALRRLHESGALTTVHPENDAAIASKRAEFIAAGLTAPRYHALSRPLECEAE
                     AIARMINLAQIAGNAPLYIVHLSNGLGLDYLRLARANHQPVWVETCPQYLLLDERSYD
                     TEDGMKFILSPPLRNVREQDKLWCGISDGAIDVVATDHCTFSMAQRLQISKGDFSRCP
                     NGLPGVENRMQLLFSSGVMTGRITPERFVELTSAMPARLFGLWPQKGLLAPGSDGDVV
                     IIDPRQSQQIQHRHLHDNADYSPWEGFTCQGAIVRTLSRGETIFCDGTFTGKAGRGRF
                     LRRKPFVPPVL"
     gene            complement(5601..6812)
                     /gene="ygeY"
                     /locus_tag="ACHKBL_003795"
     CDS             complement(5601..6812)
                     /gene="ygeY"
                     /locus_tag="ACHKBL_003795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HFO5820601.1"
                     /translation="MAKNIPFKLILEKAKDYQADMTRFLRDMVAIPSESCDEKRVVHR
                     IKEEMEKVGFDKVEIDPMGNVLGYIGHGPRLVAMDAHIDTVGIGNIKNWDFDPYEGME
                     TDELIGGRGTSDQEGGMASMVYAGKIIKDLGLEDEYTLLVTGTVQEEDCDGLCWQYII
                     EQSGIRPEFVVSTEPTDCQVYRGQRGRMEIRIDVQGVSCHGSAPERGDNAIFKMGPIL
                     GELQELSQRLGYDEFLGKGTLTVSEIFFTSPSRCAVADSCAVSIDRRLTWGETWEGAL
                     DEIRALPAVQKANAVVSMYNYDRPSWTGLVYPTECYFPTWKVEEDHFTVKALVNAYEG
                     LFGKAPVVDKWTFSTNGVSIMGRHGIPVIGFGPGKEPEAHAPNEKTWKSHLVTCAAMY
                     AAIPLSWLATE"
     gene            complement(6870..8066)
                     /gene="dpaL"
                     /locus_tag="ACHKBL_003796"
     CDS             complement(6870..8066)
                     /gene="dpaL"
                     /locus_tag="ACHKBL_003796"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008838 - diaminopropionate ammonia-lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HFO5820602.1"
                     /translation="MSVFSLKIDIADNKFFNGETSPLFSQSQAKLARQFHQKIAGYHP
                     TPLCALDDLANLFGVKKILVKDESKRFGLNAFKMLGGAYAIAQLLCEKYHLDIETLSF
                     EHLKNAIGEKMTFATTTDGNHGRGVAWAAQQLGQNAVIYMPKGSAQERVDAILNLGAE
                     CIVTDMNYDDTVRLTMQHAQQHGWEVVQDTAWEGYTKIPTWIMQGYATLADEAVEQMR
                     EMGVTPTHVLLQAGVGAMAGGVLGYLVDVYSPQNLHSIIVEPDKADCIYRSGVKGDIV
                     NVGGDMATIMAGLACGEPNPLGWEILRNCATQFISCQDSVAALGMRVLGNPYGNDPRI
                     ISGESGAVGLGVLAAVHYHPQRQSLMEKLALNKDAVVLVISTEGDTDVKHYREVVWEG
                     KHAVAP"
     gene            complement(8124..9311)
                     /gene="ygeW"
                     /locus_tag="ACHKBL_003797"
     CDS             complement(8124..9311)
                     /gene="ygeW"
                     /locus_tag="ACHKBL_003797"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="knotted carbamoyltransferase YgeW"
                     /protein_id="HFO5820603.1"
                     /translation="MKTVNELIKDINSLTSHLHEKDFLLTWEQTPDELKQVLDVAAAL
                     KALRAENISTKVFNSGLGISVFRDNSTRTRFSYASALNLLGLAQQDLDEGKSQIAHGE
                     TVRETANMISFCADAIGIRDDMYLGAGNAYMREVGAALDDGYKQGVLPQRPALVNLQC
                     DIDHPTQSMADLAWLREHFGSLENLKGKKIAMTWAYSPSYGKPLSVPQGIIGLMTRFG
                     MDVTLAHPEGYDLIPDVVEVAKNNAKASGGSFRQVTSMEEAFKDADIVYPKSWAPYKV
                     MEERTELLRANDHEGLKALEKQCLAQNAQHKDWHCTEEMMELTRDGEALYMHCLPADI
                     SGVSCKEGEVTEGVFEKYRIATYKEASWKPYIIAAMILSRKYAKPGALLEQLLKEAQE
                     RVK"
BASE COUNT         2235 a         2333 c         2513 g         2518 t
ORIGIN      
        1 cgtttaacta cgttttctaa atttcagtat accttttctt cgctgtaata aagtcgtcag
       61 gagtatcaat atctaaaatt atttctgcat tttctatttc gacggaataa tgttctccca
      121 taaggagtgc ctgacgcata ttggtgacat tgggtcgctg gattgcctgc atcaggcatg
      181 gttttgacac taaaatcgga tggccgggga tgccattatg gagtggcagt attgcgccat
      241 catttcgtaa cgaccagatt tttctaaaaa tatcgatggt gagggttggc atatctccgt
      301 gggtgagaaa acaatgttct gtttgcaccg cgggtactgc ggcctttact gatgtcagta
      361 aaccctgcgc ataatctggg ttgtggataa tagtgatatt gctctggttc gcataacgtt
      421 cgtgcagttc attaccacga tagccggtga ctaaaataat tcggctacaa aactgcaacg
      481 catttttgat acttgtatca agaattgttc cctgttgcca gggtaacata attttccatt
      541 gccccattct tgatgataat cctgcggcgg taattataca gtcgatggct gacatagtga
      601 ttccattatt tacaggagct aataacgcct gataattgcg ttacttcctg atggattaag
      661 ttgagggtaa tttatcccag agaggtccat aaagacgcgt gaaaagtata attgacccat
      721 cggcattatt cattgattta ggcgcgcaga aacgtcccac tgtgatttct gttgttggtg
      781 ctggggggaa aaccagcttg cttttttggc tggcagaact gttccaggcg agcggcaggc
      841 gtgtattaat cactacgacg acacatatgt ttatgccaac atctcactgg cccgtggttt
      901 tctgtcgtga tcccgccatg cttcctcatg cgtcttttat atcccccatt tcattttgtt
      961 ttcacagctg gaaagcgaac cagggaaaag tgcagggatt tacgccagaa gcgattgatg
     1021 cactggtaca acgatcagag tgtgacgtaa ttctcattga ggcagatggc tcgcgtggaa
     1081 tgccgttaaa agcgcctgat gagcacgaac cttgcatacc taaaagcagt tgctgcgtga
     1141 ttgctgtgat gggaggacat attttgggtg cgaaagtgag cacagaaaat gtccatcgct
     1201 ggtcgcagtt tgctgatatt accgggttaa cacctgatgc aaccttgcaa ctgagcgatc
     1261 tcgttgcgct ggttcgccac cctcaggggg cgtttaaaaa cgtaccgcaa ggttgtcggc
     1321 gagtctggtt cattaaccgt ttttctcaat gtgagaatgc gattgcgcaa agcgagctcc
     1381 ttcaaccgct gcaacaacac gacgtagagg caatctggct gggcgatata caagaacatc
     1441 ctgcaatcgc gcgcagattt gtgaattagc ggcccgggta ttccgggtta ctgaaaggat
     1501 atccattgag gttggtatga atattttcac agaggctgca aaactcgaag agcaaaattg
     1561 tccgtttgcg atggcgcaaa ttgttgatag ccgaggctca accccccgcc attctgcaca
     1621 aatgttagtg cgcgccgatg gctctatcgt cggtacaatt ggtggcggaa tggttgagcg
     1681 gaaggtgatt gaagagtcgc ttcaggcatt gcaggaacgt aagccgcgat tattccatgg
     1741 acgtatggct cgtaacggtg cggatgctgt cgggtcagat tgtggaggtg caatgtcagt
     1801 atttatcagc gtccatggta tgcgtccacg tctggtgttg attggcgcgg ggcatgtcaa
     1861 ccgggcgata gcccagagtg cggcgctatt aggatttgat atcgccgttg ccgatattta
     1921 tcgcgaaagc ctcaatcctg aactattccc accatcaacc acgcttctcc atgctgagtc
     1981 gtttggtgcg gcagtggaag cactggatat tcgccctgat aattttgtcc tgattgccac
     2041 gaataatcag gatcgtgaag ccctcgacaa actcattgaa caacccattg catggctggg
     2101 gttgctggca agtcgccgca aggttcagct tttcctgcgc caattgcgtg agaaaggcgt
     2161 ggctgaagaa catattgccc gtttacatgc gcccgttggt tacaacatag gtgcggaaac
     2221 gccgcaggag atcgccatca gcgtgctggc agaaatatta caggtgaaaa ataacgcgcc
     2281 gggtgggctg atgatgaagc cttctcatcc ttccggacac cagctggtgg tgattcgtgg
     2341 tgcgggggat atcgccagtg gtgtagcgct acgtctgtat catgcgggtt ttaaagtgat
     2401 catgctggaa gtggaaaaac cgacagtgat tcgttgtacc gtggcatttg cccaggccgt
     2461 gttcgatggc gaaatgacgg tcgaaggcgt caccgcccgc ctggcaacca gctctgcgga
     2521 ggcgatgaaa cttaccgaat gcggattcat ccctgtgata gtagatcccg cctgttcatt
     2581 gcttgatgaa ctgaaaccgc tttgcgtggt ggacgctatt ctggcgaaac agaatttggg
     2641 gacgcgggca gatatggcac cagtaacaat cgcgcttggg ccgggcttta ctgcagggaa
     2701 ggattgtcat gcggtaattg aaacaaatcg cgggcactgg ctcggtcagg tgatttactc
     2761 tggttgtgcg caggagaata ccggtgttcc tggcaatatt atggggcata ccacccgacg
     2821 ggtgatccgt gctcctgctg caggcattat gcgatccaac gtgaaattag gcgatctggt
     2881 gaaagagggc gatgtgattg cctggattgg tgagcatgaa attaaagcac cgttgacggg
     2941 gatggtgcgt ggcttgttga acgacggact ggcagtggtc ggtggtttta aaattggtga
     3001 tatcgatcct cgtggtgaaa cggctgattt caccagcgtt tctgataaag cccgggcgat
     3061 tggcggcggc gtacttgagg cgttaatgat gttgatgcat cagggcgtta aagcgacgaa
     3121 agaagtgctg gaagtggctt aaatgaaaat ggccggatgg tattacccac ccggccttta
     3181 aattactgac aaatcaccgt tccggttttg ccttcaatcc cttctttcgc tttgctcaat
     3241 acggtaatca gtgcttcacg acctgcgcga gagcgagcaa acgaggcggc agcttccact
     3301 ttaggcagca tagaaccttt cgcaaaatga ccttcttcga taaagcgttc agcatcactt
     3361 aatgacaggc gatcgagcca ctgttcattc tctttaccga agttaatggc cactttttct
     3421 accgcagtca gaatgatcag catatcggca tcaatcattt ctgctaaacg ggcgctggcc
     3481 cagtctttat cgataaccgc actggcaccg cgcagatggt tcccttcacg aataacggga
     3541 atgccaccac cgccaacggt gatcactacc tggcctgctt ctaccagcgc ttttaccgtc
     3601 tctttttcaa taatatcaac cggtttcggc gaggcaacaa cacggcgata gccgcgacca
     3661 gcatcttctt tcagagtgta accctgtttt gtcagttgct ctgcttcctg ctcggtaaag
     3721 aacgaaccga tcggcttggt cgggttgagg aatgccgggt cgttagcatc gacttcgacc
     3781 tgggtaacca gcgttgctac aggtttatta atgccacgag aaagcagttc ttctcttaat
     3841 gcgttttgta gatcgtaacc aatataaccc tggcttaagg caacacagac agacatcggc
     3901 agcattggcg agtgcgcttc agtttttgcg gcagcttcaa aggcctggtt aatcatgccc
     3961 acctgtggcc cgttaccgtg agtgacgata acttcatgtc cctgggcaat taaatcaaca
     4021 attgcctgag aagtaatttt taccgctttc atttgtccgg ccaggtcatc gcccagcgca
     4081 ttcccgccca gggcgagaac aattttctta ctcatttttc ctcactcatc attcttgatt
     4141 gtgtatagat ccctggcagt ggttagagca cgggagggac aaacggtttg cgtcgcagga
     4201 aacgacctcg cccggctttg cctgtaaagg tgccgtcaca gaaaatcgtt tcaccacggg
     4261 ataaagttct gacaatcgcg ccctgacagg taaaaccctc ccatggcgag tagtcggcgt
     4321 tgtcgtggag atggcgatgc tgaatttgtt gactctgacg tgggtcgata atcaccacgt
     4381 cgccatcgga accgggcgct aataatcctt tttgcggcca caggccaaac agcctggcgg
     4441 gcatcgcgct ggttaattca acaaaacgtt ccggtgttat acgtcccgtc ataacgccac
     4501 tggaaaacag taactgcatg cggttttcca caccgggtaa gccatttggg cagcgactga
     4561 aatcgccttt agaaatttgc aggcgttgag ccatcgagaa tgtgcagtga tcggtcgcta
     4621 ccacgtcaat cgcaccatcg ctgatgccac accacagttt gtcctgctcg cgtacgttac
     4681 gcagcggtgg gctaagaatg aacttcatgc catcttctgt atcgtaactg cgttcgtcca
     4741 acaggagata ttgtgggcag gtttcaaccc agactggctg gtgattcgca cgggcaagac
     4801 gcagataatc cagacctaag ccgttagaca ggtgcacgat atagagcggg gcgttaccgg
     4861 caatttgtgc caggttaatc atgcgggcga tggcttccgc ttcgcattcc agaggtcgac
     4921 tcaaggcatg ataacgcggc gcggttaacc ctgcggcgat aaactccgcc cgcttgctgg
     4981 cgatagctgc gtcattttcc gggtgcacgg tggtcagcgc gccggattca tgcaagcggc
     5041 gtaatgcctg caacacttca tcatcgttga gtttatattg ataggttaaa tagagtttaa
     5101 aactgctcag tccttcctca accatcatcg gaatttcgtc gaggatagcg tgattgatat
     5161 gttgaatgac accgtgaaag ctgtagtcga tgactgcttt gtgggcggca taaccgcgat
     5221 aaacttccag ttgatggcgt aaccggcagc cattcgggcc aaatcccata tggtcaataa
     5281 tggttgttgt accgccacac gcagctgcgc gggtaccggt aaaaaaatca tcacaactgc
     5341 gcgcgatgcc gacatcaata ttgaaatgcg tatggacatc cacgccgcca gggaaaacgt
     5401 aacagccagt ggcatcaatt tcttcatacg ggacctgcgg cgaaatattg ctgcccaatt
     5461 ggcgaacaat cccgctctca atcagcaaat cttgtttggc ttgtccatct gcgttaacga
     5521 cagtgccgtt tttgatcaat acgcgcatag caaactccaa aaaccctccg gcgaaccgga
     5581 ggggaataag tgaagagtaa ttattcggtt gccagccagc ttaacgggat tgcagcgtac
     5641 atcgcggcac aggtcaccag gtgagatttc caggtttttt cgttaggtgc atgcgcttca
     5701 ggttctttac ccgggccaaa gccgatcacc ggaatgccgt gacggcccat gatagatacg
     5761 ccgttagttg agaaggtcca cttatcaaca accggcgctt tgccaaacag accttcgtag
     5821 gcattcacca gtgctttaac ggtgaagtga tcttcttcca ctttccaggt cgggaagtag
     5881 cattcggttg ggtaaaccag gccagtccag gacggacggt cgtagttgta catagaaaca
     5941 accgcgttag ctttctgtac tgcaggcagg gcgcggattt cgtccagcgc gccttcccag
     6001 gtttcgcccc aggtcagacg gcggtcaata gagacggcgc agctatctgc tacagcgcaa
     6061 cggcttgggg atgtgaagaa gatttcagaa acggtgaggg tgcctttgcc gaggaattcg
     6121 tcataaccca gacgttggga gagttcttgt aattcgccaa gaatcggacc cattttgaaa
     6181 atggcgttgt caccgcgttc tggtgcagaa ccgtggcagc tcacaccctg aacatcaata
     6241 cgaatttcca tacgaccgcg ctgaccacgg tatacctggc agtcggttgg ttcggtactg
     6301 accacaaatt ccgggcgaat gccggattgt tcaataatgt actgccagca caggccgtcg
     6361 cagtcttctt cctgcacagt accggtaacc agcagggtat attcatcttc cagaccgagg
     6421 tctttaatga ttttaccggc ataaaccata gatgccatgc cgccttcctg gtcggaagta
     6481 ccgcgaccac cgatcagctc atcagtttcc atgccttcgt acggatcgaa gtcccagttt
     6541 ttgatgttgc caatgccgac ggtatcgata tgagcgtcca ttgccaccag acgcgggccg
     6601 tggccgatat aaccgagaac gttgcccatc gggtcgattt caactttatc gaagccgact
     6661 ttttccatct cttctttaat acgatgtact acgcgtttct cgtcgcagct ttcactggga
     6721 atagcaacca tatcgcgcag gaagcgagtc atgtctgcct ggtaatcttt tgctttttca
     6781 agaatcagtt tgaatggaat attcttagcc attatttttc tccagatttt ttactccccg
     6841 ttttgcaggg agcagttcca atagggtgat taaggtgcta cagcgtgttt gccttcccag
     6901 acaacttcgc ggtagtgctt cacgtcggtg tcgccttcag tgctgataac cagcaccacg
     6961 gcatctttgt tcagcgccag tttttccatc aggctttgac gttgcgggtg ataatgaacc
     7021 gctgcgagaa cgcccaaacc gacagcgccg gattcaccgg agatgatgcg cgggtcgttg
     7081 ccatacggat tacccagcac gcgcatacct aatgcggcaa cgctgtcctg gcaggagatg
     7141 aattgggtgg cgcagttacg taggatttcc cagcccagtg ggttaggttc gccgcaggcc
     7201 aggcccgcca tgatggtggc catatcaccg ccaacgttga cgatgtcgcc tttgacgccg
     7261 gagcgataaa tacagtcagc tttgtcaggt tcaacaataa tgctgtgcag attttgcggg
     7321 ctatagacgt cgaccagata acccagcaca ccaccggcca ttgcaccgac accggcttgc
     7381 agcagaacgt gtgtcggggt gacgcccatt tcacgcattt gctcgacggc ttcatccgcc
     7441 agtgttgcgt agccttgcat gatccaggtt gggattttgg tgtaaccttc ccatgccgtg
     7501 tcctgtacca cttcccagcc gtgctgctgc gcgtgttgca tggtcaggcg aacggtatcg
     7561 tcatagttca tatccgtgac gatgcactcg gcaccgaggt ttagaatggc gtcaacgcgt
     7621 tcctgagcag aacctttcgg catgtaaatc accgcattct gtccgagttg ctgtgctgcc
     7681 catgccacac cgcgcccgtg gttgccgtcg gtggtagtcg cgaaagtcat tttttcgccg
     7741 atggcatttt tcaggtgctc aaatgacagc gtttcgatat caagatgata tttttcacac
     7801 aataattgag cgatggcgta cgcaccgcca agcattttga aggcgttcag accgaatcgt
     7861 tttgattcgt ctttgacgag aattttcttt acaccaaaca ggtttgcgag atcgtccagc
     7921 gcacaaagcg gtgttgggtg ataaccagca attttctggt ggaactggcg tgccagtttt
     7981 gcctggcttt gcgaaaaaag cggtgatgtt tcgccgttga aaaatttgtt atcggcgata
     8041 tcaatcttca atgagaaaac ggacatatcc tatccttttg gcctctggaa atagagggcc
     8101 gaccagctga tcggcccgag gggttatttc acgcgttctt gcgcttcttt cagcagttgc
     8161 tcgagcagtg cacctggttt ggcgtattta cgggacagga tcatcgcggc gatgatataa
     8221 ggcttccagc tggcttcttt gtaggtagcg atacggtatt tttcgaatac gccttcagtc
     8281 acttcacctt ctttgcagga tacgccgctg atatcagctg gcaggcagtg catgtacagg
     8341 gcttcgccat cacgggtcag ttccatcatc tcttcagtac aatgccagtc tttgtgttgc
     8401 gcgttctgtg ccagacactg tttttccagt gcttttaagc cttcgtgatc gttcgcacgc
     8461 agcaattcag tacgctcttc catcactttg taaggtgccc atgacttcgg ataaacgatg
     8521 tctgcgtctt tgaaggcttc ttccatgctg gtgacctgac ggaagctacc accggaggct
     8581 ttagcattgt ttttagcaac ttcaacgaca tccgggatca ggtcgtagcc ttccggatgg
     8641 gccagggtga catccatacc gaagcgggtc atcagaccga tgatgccttg tggtacagag
     8701 agcggtttgc catagcttgg ggagtaggcc caggtcatgg cgattttttt gcctttcagg
     8761 ttttccagcg aaccaaagtg ttcacgcaac catgccaggt cagccataga ctgagtcggg
     8821 tggtcaatat cgcattgcag gttcactaaa gccggacgct gtggcagaac cccttgtttg
     8881 tagccgtcat caagtgccgc gccaacttca cgcatatagg cgttgcctgc gcccagatac
     8941 atatcgtcgc gaataccgat ggcatcggcg cagaaggaga tcatattggc ggtttcacgc
     9001 acggtttcgc cgtgagcgat ttgtgatttg ccttcgtcga gatcttgctg tgcaaggccg
     9061 agcaggttaa gcgcggaagc ataagagaag cgggtacggg tggagttgtc gcggaatacg
     9121 gaaataccta atccactatt aaagactttg gttgagatgt tttcagcacg cagtgctttt
     9181 aatgctgcgg caacgtccag tacttgtttc agttcatctg gcgtctgttc ccacgttaac
     9241 aaaaaatctt tctcgtgaag gtgagaggtc agcgaattga tatccttaat cagctcatta
     9301 acagttttca tcataaagtc ccggtagtgg aatgacaggc aaagtggttt atttcactgt
     9361 gacttgccaa cgtgattggt gttttcacag ctatacctgg gactaacaat aagtgtgcca
     9421 gaattatcca cggggctgca aaagactaat ttgacggata atgtgtgggg ttgatcacaa
     9481 attgatgcca gcttctggtt gtttgtaaaa tacgaaaacg tttggctttt tttatcgcgt
     9541 tatttatttg ctgctaagta tcagaaataa gataaaggga caggcgatct ctcttcagt
//
LOCUS       DAZFLD010000177         9563 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.178, whole genome shotgun sequence.
ACCESSION   DAZFLD010000177 DAZFLD010000000
VERSION     DAZFLD010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9563
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.178"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            126..557
                     /gene="osmC"
                     /locus_tag="ACHKBL_003798"
     CDS             126..557
                     /gene="osmC"
                     /locus_tag="ACHKBL_003798"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin OsmC"
                     /protein_id="HFO5820604.1"
                     /translation="MTIHKKGQAHWEGDIKRGKGTVSTESGVLNQQPYGFNTRFEGEK
                     GTNPEELIGAAHAACFSMALSLMLGEAGFTPTSIDTTADVSLDKVDAGFAITKIALKS
                     EVAVPGIDASTFDGIIQKAKAGCPVSQVLKAEITLDYQLKS"
     gene            complement(613..1539)
                     /gene="ddpF"
                     /locus_tag="ACHKBL_003799"
     CDS             complement(613..1539)
                     /gene="ddpF"
                     /locus_tag="ACHKBL_003799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HFO5820605.1"
                     /translation="MSDTLLTLRDVHINFPARKNWLGKTTEHVHAINGIDLQIRRGET
                     LGIVGESGCGKSTLAQLLMGMLQPSHGQYIRSGSQRIMQMVFQDPLSSLNPRLPVWRI
                     ITEPLWIAKRSSEQQRRALAEELAVQVGIRPEYLDRLPHAFSGGQRQRIAIARALSSQ
                     PDVIVLDEPTSALDISVQAQILNLLVTLQENRELTYVLISHNVSVIRHMSDRVAVMYL
                     GQIVELGDAQQVLTAPAHPYTRLLLDSLPAIDKPLEEEWALRKTDLPGNRTLPQGCFF
                     RERCPLTTHGCEVRQSLTIREDEREIRCWRAL"
     gene            complement(1532..2518)
                     /gene="ddpD"
                     /locus_tag="ACHKBL_003800"
     CDS             complement(1532..2518)
                     /gene="ddpD"
                     /locus_tag="ACHKBL_003800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO5820606.1"
                     /translation="MTQPVLDIQQLHLSFPGFNGDVHALNNVSLKINRGEIVGLVGES
                     GSGKSVTAMLIMRLLPTGSYCVHRGHISLLGEDVLNAREKQLRQWRGARVAMIFQEPM
                     TALNPTRRIGLQMMDVIRHHQPISRREARAKAIALLEEMQIPDAVEVMSRYPFELSGG
                     MRQRVMIALAFSCEPQLIIADEPTTALDVTVQLQVLRLLKHKARASGTAVLFISHDMA
                     VVSQLCDSVYVMYAGSVIESGVTADVIHHPRHPYTIGLLQCAPEHGIPRQPLPAIPGT
                     VPNLTHLPDGCAFRDRCYAAGAQCENVPVLTAYGDNNHRCACWYPQQEVISV"
     gene            complement(2515..3411)
                     /gene="ddpC"
                     /locus_tag="ACHKBL_003801"
     CDS             complement(2515..3411)
                     /gene="ddpC"
                     /locus_tag="ACHKBL_003801"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D,D-dipeptide ABC transporter permease"
                     /protein_id="HFO5820607.1"
                     /translation="MMLSEETSAVRPPKQTRFNGAKLVWMLKGSPLTVTGAVIIVLML
                     LMMIFSPWLATHDPNAIDLTARLLPPSAAHWFGTDEVGRDLFSRVLVGSQQSILAGLV
                     VVAIAGMMGSLLGCLSGVLGGRADAIIMRIMDIMLSIPSLVLTMALAAALGPSLFNAM
                     LAIAIVRIPFYVRLARGQTLVVRQYTYVQAAKTFGASRWHLINWHILRNSLPPLIVQA
                     SLDIGSAILMAATLGFIGLGAQQPSAEWGAMVANGRNYVLDQWWYCAFPGAAILLTAV
                     GFNLFGDGIRDLLDPKAGGKQS"
     gene            complement(3408..4430)
                     /gene="ddpB"
                     /locus_tag="ACHKBL_003802"
     CDS             complement(3408..4430)
                     /gene="ddpB"
                     /locus_tag="ACHKBL_003802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO5820608.1"
                     /translation="MTFWSILRQRCWGLVLVVAGVCVITFIISHLIPGDPARLLAGDR
                     ASDAIVDNIRQQLGLDQPLYVQFYRYVSDLFQGDLGTSIRTGRPVLEELRIFFPATLE
                     LAFCALLLALLIGIPLGILSAVWRNRWLDHLVRIMAITGISTPAFWLGLGVIVLFYGH
                     LQILPGGGRLDDWLDPPTHVTGFYLLDALLEGNGEVFFNALQHLILPALTLAFVHLGI
                     VARQIRSAMLEQLSEDYIRTARASGLPGWYIVLCYALPNALIPSITVLGLALGDLLYG
                     AVLTETVFAWPGMGAWVVTSIQALDFPAVMGFAVVVSFAYVLVNLVVDLLYLWIDPRI
                     GRGGGE"
     gene            complement(4432..5982)
                     /gene="ddpA"
                     /locus_tag="ACHKBL_003803"
     CDS             complement(4432..5982)
                     /gene="ddpA"
                     /locus_tag="ACHKBL_003803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFO5820609.1"
                     /translation="MKRSILFRPTLLAIVLATTMPVAHAAVPKDMLVIGKAADPQTLD
                     PAVTIDNNDWTVTYPSYQRLVQYKTDGDKGSTDVEGDLASSWKASDDQKEWTFTLKDN
                     AKFADGTPVTAEAVKLSFERLLKIGQGPAEAFPKDLKIDAPDEHTVKFTLSHPFAPFL
                     YTLANDGASIINPAVLKEHAADDARGFLAQNTAGSGPFMLKSWQKGQQLVLVPNPHYP
                     GNKPNFKRVSVKIIGESASRRLQLSRGDIDIADALPVDQLNALKQENKVNVAEYPSLR
                     VTYLYLNNSKAPLNQADLRRAISWSTDYQGMVNGILSGNGKQMRGPIPEGMWGYDATA
                     MQYNHDETKAKAEWDKVTSKPTSLTFLYSDNDPNWEPIALATQSSLNKLGINVKLEKL
                     ANATMRDRVGKGDYDIAIGNWSPDFADPYMFMNYWFESDKKGLPGNRSFYENSEVDKL
                     LRNALATTDQTQRTRDYQQAQKIVIDDAAYVYLFQKNYQLAMNKEVKGFVFNPMLEQV
                     FNINTMSK"
     gene            complement(5996..6577)
                     /gene="ddpX"
                     /locus_tag="ACHKBL_003804"
     CDS             complement(5996..6577)
                     /gene="ddpX"
                     /locus_tag="ACHKBL_003804"
                     /EC_number="3.4.13.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine dipeptidase"
                     /protein_id="HFO5820610.1"
                     /translation="MSDTTELVDLAVIFPDLEIELKYACADNITGKAIYQQARCLLHK
                     DAITALAKSISIAQLSGLQLVIYDAYRPQQAQAMLWQACPDPQYVVDVTVGSNHSRGT
                     AIDLTLRDEHGNILDMGAGFDEMHERSHAYHPSVPPAAQRNRLLLNAIMTGGGFVGIP
                     SEWWHFELPLAASYPLLADQFTCFISPGTQHAS"
     gene            complement(6835..9234)
                     /gene="dosP"
                     /locus_tag="ACHKBL_003805"
                     /gene_synonym="pdeO"
     CDS             complement(6835..9234)
                     /gene="dosP"
                     /locus_tag="ACHKBL_003805"
                     /gene_synonym="pdeO"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-sensing cyclic-di-GMP phosphodiesterase
                     DosP"
                     /protein_id="HFO5820611.1"
                     /translation="MKLTDADNAADGIFFPALEQNMMGAVLINENDEVMFFNPAAEKL
                     WGYKREEVIGNNIDMLIPRDLRPAHPQYIRHNREGGKARVEGMSRELQLEKKDGSKIW
                     TRFALSKVSAEGKVYYLALVRDASVEMAQKEQTRQLIIAVDHLDRPVIVLDPERHIVQ
                     CNRAFTEMFGYCINEASGMQPDTLLNIPEFPADNRIRLQQLLWKTARDQDEFLLLTRT
                     GEKIWIKASISPVYDVLAHLQNLVMTFSDITEERQIRQLEGNILAAMCSSPPFHEMGE
                     IICRNIESVLNESHVSLFALRNGMPIHWASSSHGAEVQNAQSWSATIRQRDGAPAGIL
                     QIKTSSGAETSAFIERVADISQHMAALALEQEKSRQHIEQLIQFDPMTGLPNRNNLHN
                     YLDDLVDKAVSPVVYLIGVDHIQDVIDSLGYAWADQALLEVVNRFREKLKPDQYLCRI
                     EGTQFVLVSLENDVSNITQIADELRNVVSKPIMIDDKPFPLTLSIGISYDVGKNRDYL
                     LSTAHNAMDFIRKNGGNGWQFFSPAMNEMVKERLVLGAALKEAISNNQLKLVYQPQIF
                     AETGELYGIEALARWHDPLHGHVPPSRFIPLAEEIGEIENIGRWVIAEACRQLAEWRS
                     QNIHIPALSVNLSALHFRSNQLPNQVSDAMQAWGIDGHQLTVEITESMMMEHDTEIFK
                     RIQILRDMGIGLSVDDFGTGFSGLSRLVSLPVTEIKIDKSFVDRCLTEKRILALLEAI
                     TSIGQSLNLTVVAEGVETKEQFEMLRKIHCRVIQGYFFSRPLPAEEIPGWMSSMLPLK
                     I"
     gene            complement(9259..9423)
                     /locus_tag="ACHKBL_003806"
                     /pseudo
     CDS             complement(9259..9423)
                     /locus_tag="ACHKBL_003806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310122.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
BASE COUNT         2348 a         2496 c         2428 g         2291 t
ORIGIN      
        1 gtggaaattt aatttaagtg caggaattat attttcgccg gattttattc ggattatcct
       61 gcttatcctc gtgctgtttc tcacgtagtc tataatttcc tttttaagcc cacaggagag
      121 caacaatgac aatccataag aaaggtcagg cacactggga aggcgatatc aaacgcggga
      181 agggaacagt atctactgag agcggcgtgc tgaaccaaca gccgtatgga tttaacacgc
      241 gttttgaagg cgaaaaagga accaaccctg aagaactgat tggcgcagcg catgccgcat
      301 gtttctcaat ggcgctttca ttaatgctgg gggaagcggg attcacgcca acatcgattg
      361 ataccaccgc agatgtgtcg ctggataaag tggatgccgg ttttgcgatt actaaaatcg
      421 cactgaaaag cgaagttgcg gtgccgggta ttgatgcctc cacctttgac ggcattatcc
      481 agaaagcaaa agcaggatgc ccggtttctc aggtactgaa agcggaaatt acgctggatt
      541 accagttgaa atcgtaaagc gttgccggat gacgcgtcag gcgtgtgatt gcctgatgcg
      601 ttgttagcgt ctctacagcg cccgccagca ccggatctca cgttcgtcct cccttattgt
      661 taatgattgc cggacttcac atccgtgggt tgtcagaggg cagcgttcgc ggaaaaaaca
      721 gccttgtggc aacgtgcggt ttcctggtag atccgtttta cgtaatgccc attcttcctc
      781 cagcggttta tcaatggcgg ggagggaatc cagcaataat cgggtgtatg gatgtgccgg
      841 agctgtcagc acctgttgcg cgtctcctag ttccacaatc tgtccgagat acatcaccgc
      901 cacccgatcg ctcatatgac gtatcaccga gacattgtgt gaaatcagca cataagtcag
      961 ctcgcgattt tcctgtagcg ttaccagtaa attgaggatc tgcgcctgca cggagatatc
     1021 cagcgccgag gttggttcat caagcacaat cacgtcaggc tgcgaagaga gtgctctggc
     1081 aatggcgata cgttgccgct gcccgccgga aaacgcatga ggcaagcggt cgagatactc
     1141 cggacgaata cccacctgca cagccagctc ctctgccaac gctcgccgct gttgttcact
     1201 actacgctta gcaatccaga gcggttccgt gatgatgcgc cacaccggta agcgcggatt
     1261 aagcgaagag agcgggtcct gaaacaccat ctgcataatg cgttgtgatc ctgaacggat
     1321 gtactgcccg tggctcggtt gcagcatacc cattaaaagc tgtgcgaggg tgcttttgcc
     1381 gcagcctgac tcaccgacga tccctaaggt ttcaccacga cggatctgta aatcaatacc
     1441 attgatggca tgaacatgtt ccgtagtttt accaagccag tttttacggg ccgggaaatt
     1501 gatatggacg tcgcgtaacg ttaataatgt atcagacact aatgacctcc tgctgcggat
     1561 accaacaggc gcagcggtgg ttgttgtcac catacgctgt tagcaccggg acgttttcac
     1621 actgtgcacc tgccgcatag caacgatcgc gaaaagcgca gccgtcaggc aaatgggtga
     1681 ggtttggtac cgtccccgga atagcgggta atggctggcg cggtattcca tgttccggtg
     1741 cgcattgcag caaaccaatg gtatacggat gccggggatg atggataacg tctgccgtca
     1801 cgccgctctc aatcacgctt ccggcataca tcacgtaaac gctatcgcac agttgcgaca
     1861 ccacggccat atcatggctg atgaacagta ccgcagttcc actggcgcgg gctttatgtt
     1921 taagcagacg cagtacctgc aactgtaccg tgacgtccag cgccgtagtc ggttcgtcgg
     1981 caataatcaa ttgcggctcg caggagaatg ccagcgcaat cattacccgc tggcgcatac
     2041 cacctgaaag ctcaaacgga tagcgcgaca taacttccac ggcatccggg atttgcatct
     2101 cttccagcag ggcaatcgct ttagctctgg cttcccgacg acttattggt tgatgatggc
     2161 ggatcacgtc catcatctga agacctattc gacgtgtcgg attaagggcg gtcatcggtt
     2221 cctgaaagat catcgccact cgtgcgccgc gccactgacg aagctgcttt tcccgagcgt
     2281 taagaacatc ttctcccagc agtgaaatat gtccccgatg tacgcaataa ctgcccgtcg
     2341 gtagcagacg cataatcaac attgcagtga ctgatttacc cgagccggat tctcccacca
     2401 gaccgacaat ttcaccgcgg ttgattttca atgacacatt gttgagcgcg tgaacgtcgc
     2461 cgttaaaacc ggggaaactc aaatgcagtt gttgaatgtc cagaacgggt tgggtcatga
     2521 ctgctttcct cctgctttcg ggtcaagcag atcgcgaata ccatcgccaa agagattaaa
     2581 cccgacggcg gtaagcaaaa tcgctgcccc cggaaatgcg caataccacc attgatcgag
     2641 cacatagttg cgaccattcg ccaccatcgc cccccattca gcactcggtt gttgagcacc
     2701 aaggccaata aatcccaacg tggcggccat taaaatcgcg ctaccgatat ccagcgatgc
     2761 ctgcacaatc agcggcggca gggagttacg taaaatatgc cagttgatca gatgccagcg
     2821 tgacgcacca aaggttttcg ccgcctgaac ataggtatac tggcgaacaa ctaatgtttg
     2881 cccccgcgcc aggcgcacat aaaagggaat tcgcacaata gcaatcgcca gcatggcgtt
     2941 aaacaaactc ggcccgagag cggctgccag tgccattgtc agcaccagcg aaggaatcga
     3001 cagcataatg tccatgatgc gcatgataat cgcgtctgcg cgtccgccaa gcacaccgga
     3061 tagacacccg agtagcgacc ccatcatacc tgcaatggcg accaccacta atccggcgag
     3121 aattgattgc tgactgccga ccagtacgcg gctgaacaga tcgcgtccca cttcatcggt
     3181 gccaaaccag tgcgctgcag aaggcggcaa aaggcgggcg gttaaatcaa tggcgttggg
     3241 atcatgcgtc gccagccacg gtgaaaaaat catcatcaat agcattaata caatgatgac
     3301 tgcgccggtc acggtaagcg ggctgccttt cagcatccat accagttttg caccgttaaa
     3361 tcgcgtttgt tttggtgggc gcacggctga cgtttcctcg cttagcatca ttcaccacct
     3421 ccacgtccga tacgcggatc aatccacaaa tagagcaaat ccaccaccag gttcaccagc
     3481 acataagcaa acgaaaccac gacggcaaag cccatcactg ccgggaagtc gagcgcctgt
     3541 attgatgtta ctacccatgc acccattccg ggccaggcaa aaacggtttc ggtgagcact
     3601 gcgccataca acaaatcgcc cagcgccagt cccaatacgg taatcgaggg gatcaacgca
     3661 ttgggtagcg cataacataa aacgatatac cagccgggca agccgctggc ccgggcggta
     3721 cgaatgtaat cttcactcaa ttgttccagc atagctgagc ggatctggcg agcgacaatt
     3781 cccaggtgaa cgaacgccag cgttaatgcc ggtaagatga gatgttgcag cgcattgaag
     3841 aagacttcac cgttgccttc aagcagcgca tcgagcagat aaaagccggt aacgtgcgtt
     3901 ggtggatcca gccagtcatc aagccttccg ccgccgggaa gaatttgcag atgaccataa
     3961 aacagcacaa tgacgcccag cccaagccag aacgcaggtg tggagattcc ggtaatggcc
     4021 attattcgca ccagatgatc cagccagcga tttcgccaga ctgcagagag tatgcccagc
     4081 gggatgccaa tcagaagtgc cagcagcagg gcacaaaaag ccagttccag cgtcgccggg
     4141 aaaaatatgc gcaactcttc cagcaccgga cgcccggtac gaatggatgt tcccaggtca
     4201 ccctgaaaca ggtcgctgac gtagcggtaa aactgtacgt acagtggctg gtccagtccc
     4261 agttgctggc gaatattatc cacgatagca tcgctggcgc ggtcacccgc cagtaaccgc
     4321 gccggatcgc cggggatcag gtgcgagata ataaacgtta tcacacagac gcccgccacc
     4381 acgagcacca gcccccagca gcgttggcgt aaaatactcc agaaggtcac gttatttact
     4441 catggtattg atattgaaga cctgttccag catgggattg aacacaaagc ctttcacctc
     4501 tttgttcatc gccagttggt agtttttctg gaacaggtac acataagcag cgtcatcaat
     4561 gacgattttc tgtgcctgct ggtagtcccg ggtacgctgc gtctggtcgg tggtcgctag
     4621 cgcattgcgc agtagcttat cgacctcact gttttcatag aacgagcggt tacccggcag
     4681 accttttttg tctgactcaa accagtaatt cataaacata tacgggtcgg caaaatccgg
     4741 actccagttg ccaatcgcga tgtcgtaatc acctttaccc actctgtcgc gcatggtggc
     4801 gttcgccagc ttttccagct tcacattgat gcccagtttg ttgagactgg attgtgtcgc
     4861 cagagcgatc ggttcccagt tcggatcatt atcagaatag agaaacgtca ggctggtggg
     4921 tttgctcgtc actttatccc attcggcttt ggctttcgtt tcgtcatggt tgtattgcat
     4981 tgccgtcgca tcgtatcccc acatgccttc cggaatcggg ccgcgcattt gttttccgtt
     5041 accactcaga atgccgttaa ccatgccctg ataatcggta gaccaggaaa tggcccgacg
     5101 cagatccgcc tgattaagtg gcgctttgct gttattcaga tacagatagg tgacgcgcag
     5161 tgacggatac tctgccacat tgactttatt ttcctgcttc agggcgttga gttgatccac
     5221 cggcagcgca tcggcaatgt caatgtcgcc acgggagagc tgcaggcgac gggaggcact
     5281 ttcaccgata atttttaccg atactcgttt aaagttcggt ttattgccgg ggtaatgcgg
     5341 atttggcacc agaactaatt gctgaccttt ttgccagctt ttcagcataa acggtccgga
     5401 gccagcggta ttttgcgcga ggaaaccacg ggcatcatcc gccgcatgct cctttaagac
     5461 cgccggattg ataatggatg caccgtcatt cgccagcgtg tagaggaacg gtgcgaatgg
     5521 gtggctaagg gtaaacttca ccgtatgttc gtcgggagca tcaatcttta aatctttggg
     5581 aaatgcttct gctggcccct ggccgatttt tagtaaccgc tcaaaagaga gttttacggc
     5641 ttcggcagtg acaggtgtgc catcggcaaa tttagcattg tccttcaggg tgaacgtcca
     5701 ctctttttga tcgtcagacg ctttccagct actagccaga tcgccttcaa cgtcggttga
     5761 gcctttatca ccgtccgttt tgtactgaac cagtcgctga taagacgggt aggtcactgt
     5821 ccagtcgtta ttatcaattg tcaccgctgg gtcgagggtt tgtgggtcgg cagctttacc
     5881 gatcaccagc atatctttcg gtacggcggc gtgcgcaacc ggcattgttg tggcaaggac
     5941 gatcgcgagc aatgtaggac gaaacaaaat cgatctcttc atgacagttt cctgattagc
     6001 tggcgtgctg tgtgccaggg gatataaaac aggtgaattg atcagcgagt agtgggtaac
     6061 tcgctgccag gggtaattcg aagtgccacc attcgctcgg gataccgaca aagccaccgc
     6121 cagtcataat cgcattcagc aacaggcgat tgcgctgagc ggcgggcggg acggaagggt
     6181 gataagcatg ggaacgttcg tgcatttcat cgaaccctgc acccatatcg aggatgttcc
     6241 cgtgctcatc acgaagcgtc aggtcgatcg ccgtgccacg gctgtgatta gaaccgaccg
     6301 tcacatcaac aacatattgc gggtctgggc aggcttgcca caacatcgcc tgtgcttgtt
     6361 gtgggcgata cgcatcgtaa atcaccagtt gtaagcctga cagctgggcg atactgatac
     6421 ttttcgccag cgcagtaatc gcatccttgt gtaacagaca acgcgcttgc tgataaatag
     6481 ctttacctgt gatgttatca gcgcaggcgt atttcaattc gatctccaga tcagggaaga
     6541 tcacggctaa atcaaccagt tcggtggtat ccgacataaa cgattcctgt ataagacaaa
     6601 aattgctgcg ctttccgctt atgcagatct catgccacgc cgggataagc gccagaatgc
     6661 tggcttaaag ttatattttt taacttttga tcaacatttg tgcagcgtag tgcagttttg
     6721 gtgcaagaga ggaagttaag gaaggaatct cccggaatcg tagctgaaat cacagtattt
     6781 aagtgacagt gtcacgttaa atgaaaaccc gcgtgtgcgg gcgagaggaa tttgtcagat
     6841 tttcagcggt aacatactgc tcatccagcc tggaatttct tcggcgggca gggggcggga
     6901 aaagaaatat ccctgaataa cgcgacagtg gatcttgcgt agcatctcaa attgctcttt
     6961 ggtttcgacg ccttccgcca cgacggttaa attaaggctt tgcccaatgc tggtaatggc
     7021 ttcaagtaag gcaaggatgc gtttttcggt cagacaacga tcgacaaaac ttttgtcaat
     7081 tttgatttcc gttaccggaa gactgactaa gcgggataat ccggaaaagc ccgtgccaaa
     7141 atcatctacc gataaaccga tgcccatatc gcgcaggatc tgaatgcgtt taaagatttc
     7201 ggtatcgtgt tccatcatca tgctttccgt gatttccacc gtcagctggt ggccgtcgat
     7261 accccaggct tgcattgcat cagacacctg attaggcagc tgattactgc gaaagtgcag
     7321 cgccgacaag ttaacggata acgccgggat atgaatattc tggctacgcc attctgctaa
     7381 ctgacggcaa gcttccgcga tgacccagcg cccaatattt tcgatttcac cgatctcttc
     7441 tgcgagagga ataaaccgtg aagggggcac atgaccatgc aggggatcgt gccagcgagc
     7501 aagggcttcg atgccgtaca gttcacccgt ttctgcgaag atttgcggct ggtaaaccag
     7561 tttcagttgg ttattgctaa ttgcttcttt cagcgccgcg cctaaaacca aacgctcttt
     7621 taccatttcg ttcatcgccg ggctgaagaa ctgccagccg ttaccgccat ttttgcgaat
     7681 aaaatccatt gcattgtgag cagtggagag caggtaatcg cggtttttac ccacgtcgta
     7741 gctgatgcca atgctcaagg tgagcgggaa tggtttatcg tcaatcatta tcggcttgct
     7801 gaccacattc cgtagctcat cggcgatctg cgtaatgtta ctgacgtcgt tttccaggct
     7861 cacgaggaca aactgcgtac cttcgatacg acagagatat tgatccggtt tgagtttttc
     7921 acgaaagcga ttgaccactt ccagcaatgc ctgatcggcc cacgcatagc caaggctatc
     7981 aatcacatcc tgaatatggt caacaccgat gagatacacc accggagaga cggctttgtc
     8041 gaccaggtca tcgaggtaat tgtgcaggtt attgcgattt ggcagaccgg tcatcggatc
     8101 aaattggatg agttgttcaa tatgctgacg gcttttttcc tgttccagcg ccagcgcggc
     8161 catatgctgg ctgatatctg ccacgcgttc gataaaggcg ctggtttctg ctcctgacga
     8221 ggttttaatt tgcaggatcc ccgcaggcgc accatcacgc tgacgaatgg tcgctgacca
     8281 gctttgcgca ttttgaactt ctgcaccgtg cgaagatgac gcccagtgta tcggcatccc
     8341 gttgcgcagc gcgaacagcg aaacatgcga ttcgttgagt acagattcga tgttacgaca
     8401 aatgatttcc cccatttcat gaaatggcgg gctgctgcac atggcggcga gaatattgcc
     8461 ttcaagctgg cggatttgcc tttcttcggt gatatccgag aatgtcatta ccaggttctg
     8521 cagatgcgcg agcacgtcat aaaccgggct gatagaggct ttaatccaga ttttttcacc
     8581 ggtgcgcgtc aacagcagaa attcgtcctg atcgcgggcg gttttccata gcaactgctg
     8641 taaacgaata cggttatcag cagggaattc tggaatgttc aggagtgtat cgggctgcat
     8701 accgctggct tcgttaatgc agtaaccaaa catttcggta aatgcgcgat tgcactgcac
     8761 aatatggcgt tccggatcga ggacaatcac cggtcggtcg agatggtcaa cggcaataat
     8821 caattgtcgg gtctgttctt tttgcgccat ttctacgctg gcatcccgta ccagcgccag
     8881 gtaataaact ttcccctcgg cgctcacttt cgatagcgca aaacgggtcc agattttact
     8941 gccgtctttt ttctccagct gcagctcccg actcatcccc tcgacgcgag ctttaccacc
     9001 ttcacggttg tgacgaatgt attgaggatg cgcaggacgc aaatcccgcg gaatcagcat
     9061 atcaatgtta ttgccaatga cttcttcacg tttgtacccc cagagcttct ctgcggcggg
     9121 gttgaaaaac atcacttcat cattttcgtt aattaacacc gcgcccatca tattttgctc
     9181 aagggcgggg aaaaaaatgc catcggcggc attatccgca tcggttagct tcatgattac
     9241 ctctgcatcc tggcgcatct aaagactggc tttacagagt tcaacacggg ttctacctcg
     9301 tcttttggcg atatacagag cttcatcggc tatttgaatg aggcgctcat agtcaggatg
     9361 accattaaac atggcggcac cgatggaaag tgagagggca atatcctcgc cgtttgcggc
     9421 tttaaaataa tgtcgacctt tatggttaaa ccgcaggcca aaatcaacca aacacaacgt
     9481 gcgagatcga ctaactgcac catattctcc tgaaatatga agatatactg aaaagaaata
     9541 agcgacttag gacagtttca atc
//
LOCUS       DAZFLD010000178         9460 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.179, whole genome shotgun sequence.
ACCESSION   DAZFLD010000178 DAZFLD010000000
VERSION     DAZFLD010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9460
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.179"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            470..715
                     /gene="yahM"
                     /locus_tag="ACHKBL_003807"
     CDS             470..715
                     /gene="yahM"
                     /locus_tag="ACHKBL_003807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YahM"
                     /protein_id="HFO5820612.1"
                     /translation="MAVQLFKTLLNQIPLLSFLQSGTLPLFGYSGRVRPMKKAHTGES
                     GLKWEAKDSSKLIGNKDHALRGLSSPMVGIRQIRLIT"
     gene            complement(732..1403)
                     /locus_tag="ACHKBL_003808"
     CDS             complement(732..1403)
                     /locus_tag="ACHKBL_003808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HFO5820613.1"
                     /translation="MMKLLHLFMVEITMDPLHAVYLTVGLFVITFFNPGANLFVVVQT
                     SLASGRRAGVLTGLGVALGDAFYSGLGLFGLATLITQCEEIFSLIRIVGGAYLLWFAW
                     CSMRRQSTPQMSTLQQPISAPWYIFFRRGLITDLSNPQTVLFFISIFSVTLNAETPTW
                     ARLMAWAGIVLASVIWRVFLSQAFSLPAVRRAYGRMQRVASRVIGAIIGVFALRLIYE
                     GVTQR"
     gene            1550..1825
                     /gene="yahO"
                     /locus_tag="ACHKBL_003809"
     CDS             1550..1825
                     /gene="yahO"
                     /locus_tag="ACHKBL_003809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family periplasmic protein YahO"
                     /protein_id="HFO5820614.1"
                     /translation="MKIISKMLVGALAFAVTNVYAAELMTKAEFEKVASQYEKIGDIS
                     TSNEMSTADAKEDLIKKADEKGADVLVLTSGQTDNKIHGTANIYKKK"
     gene            complement(1926..3512)
                     /gene="prpR"
                     /locus_tag="ACHKBL_003810"
     CDS             complement(1926..3512)
                     /gene="prpR"
                     /locus_tag="ACHKBL_003810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate catabolism operon regulatory protein
                     PrpR"
                     /protein_id="HFO5820615.1"
                     /translation="MAHPPRLNDDKPVIWTVSVTRLFELFRDISLEFDHLANITPIQL
                     GFEKAVTYIRKKLANERCDAIIAAGSNGAYLKSRLSVPVILIKPSGYDVLQALAKAGK
                     LTSSIGVVTYQETIPALVAFQKTFNLRLDQRSYITEEDARGQINELKANGTEAVVGAG
                     LITDLAEEAGMTGIFIYSAATVRQAFSDALDMTRMSLRHNTHDATRNALRTRYVLGDM
                     LGQSPQMEQVRQTILLYARSSAAVLIEGETGTGKELAAQAIHREYFARHDARQGKKSH
                     PFVAVNCGAIAESLLEAELFGHEEGAFTGSRRGGRAGLFEIAHGGTLFLDEIGEMPLP
                     LQTRLLRVLEEKEVTRVGGHQPVPVDVRVISATHCNLEEDMRQGQFRRDLFYRLSILR
                     LQLPPLRERVADILPLAESFLKVSLAALSAPFAAALRQGLQESETMLVHYDWPGNIRE
                     LRNMMERLALFLSVESTPDLTPQFLQLLLPELARESAKTPIPGLLTAQQALEKFNGDK
                     TAAANYLGISRTTFWRRLKS"
     gene            3751..4641
                     /gene="prpB"
                     /locus_tag="ACHKBL_003811"
     CDS             3751..4641
                     /gene="prpB"
                     /locus_tag="ACHKBL_003811"
                     /EC_number="4.1.3.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046421 - methylisocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylisocitrate lyase"
                     /protein_id="HFO5820616.1"
                     /translation="MYLHSPGKAFRAALSKENPLQIVGTINANHALLAQRAGYQAIYL
                     SGGGVAAGSLGLPDLGISTLDDVLTDIRRITDVCSLPLLVDADIGFGSSAFNVARTVK
                     SMIKAGAAGLHIEDQVGAKRCGHRPNKAIVSKEEMVDRIRAAVDAKTDPDFVIMARTD
                     ALAVEGLDAAIERAQAYVEAGAEMLFPEAITELAMYRQFADAVQVPILANITEFGATP
                     LFTTDELRSAHVAMALYPLSAFRAMNRAAEHVYNVLRQEGTQKSVIDTMQTRNELYES
                     INYYQYEEKLDDLFARGQVK"
     gene            4801..5970
                     /gene="prpC"
                     /locus_tag="ACHKBL_003812"
     CDS             4801..5970
                     /gene="prpC"
                     /locus_tag="ACHKBL_003812"
                     /EC_number="2.3.3.16"
                     /EC_number="2.3.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050440 - 2-methylcitrate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate synthase"
                     /protein_id="HFO5820617.1"
                     /translation="MSDTTILQNSTHVIKPKKSVALSGVPAGNTALCTVGKSGNDLHY
                     RGYDILDLAEHCEFEEVAHLLIHGKLPTRDELAAYKTKLKALRGLPANVRTVLEALPA
                     ASHPMDVMRTGVSALGCTLPEKEGHTVSGARDIADKLLASLSSILLYWYHYSHNGERI
                     QPETDDDSIGGHFLHLLHGEKPSQSWEKAMHISLVLYAEHEFNASTFTSRVIAGTGSD
                     MYSAIIGAIGALRGPKHGGANEVSLEIQQRYETPDEAEADIRKRVENKEVVIGFGHPV
                     YTIADPRHQVIKRVAKQLSQEGGSLKMYNIADRLETVMWESKKMFPNLDWFSAVSYNM
                     MGVPTEMFTPLFVIARVTGWAAHIIEQRQDNKIIRPSANYVGPEDRQFVALDKRQ"
     gene            6004..7455
                     /gene="prpD"
                     /locus_tag="ACHKBL_003813"
     CDS             6004..7455
                     /gene="prpD"
                     /locus_tag="ACHKBL_003813"
                     /EC_number="4.2.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047547 - 2-methylcitrate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019679 - propionate metabolic process,
                     methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate dehydratase"
                     /protein_id="HFO5820618.1"
                     /translation="MSAQINNIRPEFDREIVDIVDYVMNYEISSKVAYDTAHYCLLDT
                     LGCGLEALEYPACKKLLGPIVPGTVVPNGVRVPGTQFQLDPVQAAFNIGAMIRWLDFN
                     DTWLAAEWGHPSDNLGGILATADWLSRNAVASGKAPLTMKQVLTAMIKAHEIQGCIAL
                     ENSFNRVGLDHVLLVKVASTAVVAEMLGLTREEILNAVSLAWVDGQSLRTYRHAPNTG
                     TRKSWAAGDATSRAVRLALMAKTGEMGYPSALTAPVWGFYDVSFKGESFRFQRPYGSY
                     VMENVLFKISFPAEFHSQTAVEAAMTLYEQMQAAGKTAADIEKVTIRTHEACIRIIDK
                     KGPLNNPADRDHCIQYMVAIPLLFGRLTAADYEDNVAQDKRIDALREKINCFEDPAFT
                     ADYHDPEKRAIANAITLEFTDGTRFEEVVVEYPIGHARRRQDGIPKLVDKFKINLARQ
                     FPTRQQQRILEVSLDRTRLEQMPVNEYLDLYVI"
     gene            7495..9381
                     /gene="prpE"
                     /locus_tag="ACHKBL_003814"
     CDS             7495..9381
                     /gene="prpE"
                     /locus_tag="ACHKBL_003814"
                     /EC_number="6.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050218 - propionate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="propionate--CoA ligase"
                     /protein_id="HFO5820619.1"
                     /translation="MSFSEFYQRSINEPEKFWAEQARRIDWQTPFTQTLDHSNPPFAR
                     WFCEGRTNLCHNAIDRWLEKQPEALALIAVSSETEEERTFTFRQLHDEVNAVASMLRS
                     LGVQRGDRVLVYMPMIAEAHITLLACARIGAIHSVVFGGFASHSVATRIDDAKPVLIV
                     SADAGARGGKIIPYKKLLDDAISQAQHQPRHVLLVDRGLAKMARVSGRDVDFASLRHQ
                     HIGARVPVAWLESNETSCILYTSGTTGKPKGVQRDVGGYAVALATSMDTIFGGKAGGV
                     FFCASDIGWVVGHSYIVYAPLLAGMATIVYEGLPTWPDCGVWWKIVEKYQVSRMFSAP
                     TAIRVLKKFPTAEIRKHDLSSLEVLYLAGEPLDEPTASWVSNTLDVPVIDNYWQTESG
                     WPIMAIARGLDDRPTRLGSPGVPMYGYNVQLLNEVTGEPCGVNEKGMLVVEGPLPPGC
                     IQTIWGDDDRFVKTYWSLFSRPVYATFDWGIRDADGYHFILGRTDDVINVAGHRLGTR
                     EIEESISSHPGVAEVAVVGVKDALKGQVAVAFVIPKESDSLEDREVAHSQEKAIMALV
                     DSQIGNFGRPAHVWFVSQLPKTRSGKMLRRTIQAICEGRDPGDLTTIDDPASLDQIRQ
                     AMEE"
BASE COUNT         2287 a         2488 c         2566 g         2119 t
ORIGIN      
        1 agcgatttct gatagtgtct ggtttaatca gagctttatt atctgcgacg tttattttta
       61 tttaatagag ccacctgcaa gttgttattc caccagagag tgaatcgcaa agcaggtaat
      121 catttcatct gtatatctgt atgccaactg gcattccatc tcttttttcg ccgatatttt
      181 gttgtgatcg gcgacacttc gcttaaaaaa gcatcagcag tggtttcgca gctatgcggt
      241 gtataaaaaa tgatctcatg cagatgtttt gtgaatgtgt gggttgacat tcatatgaaa
      301 aaaatcataa ttccatcatg tttgtgtggg gtcttttctg tatcttacgc atcgcactca
      361 agcctgacag aaaatatgct gtaaggctca tatcaaaaac cgccattagc tcatcaggaa
      421 gagcagacga caaccaggat tgttgtatgg tacggggttc gaggcctcga tggcagtcca
      481 gcttttcaaa actcttctga atcagatccc cttattatca ttcctgcaaa gtggaacatt
      541 gccattgttt ggctattcag gacgggtcag accaatgaaa aaagcccaca caggggagag
      601 tgggctgaaa tgggaagcta aagactcaag taaacttatc ggaaataagg accacgcatt
      661 acgggggcta tcatcgccga tggtcgggat tcggcaaatc aggttgatta cgtagagagt
      721 aaattatctg ctcaccgctg cgtcacccct tcgtaaatca ggcgtagcgc gaatacacca
      781 ataattgcac caataacccg actggcaacg cgttgcatac gcccataagc acgacgcact
      841 gcgggcaaag aaaacgcctg actaagaaaa acgcgccaga taactgatgc gagcacaatc
      901 cccgcccagg ccattaaacg tgcccatgtt ggtgtttcgg catttaatgt tactgagaaa
      961 atactaataa aaaataaaac ggtttgcggg ttagagagat cggtaattaa tccacggcgg
     1021 aaaaagatat accagggggc actaatcggt tgttgtagtg tgctcatttg cggtgttgac
     1081 tggcggcgca tgctgcacca cgcaaaccat aagagataag cgccgccgac gattctgata
     1141 agcgaaaaaa tctcctcaca ctgcgtaatt agtgttgcaa gaccaaacaa acccaacccg
     1201 gaataaaatg catcgcccaa cgccacgccc agtccggtca gcacccctgc gcgtcgaccg
     1261 gaagccaggc tggtttgtac taccacaaag agattggctc ccggattaaa aaaagtaatc
     1321 acgaacagtc ctacggtcag ataaacggcg tgcaaaggat ccatagtgat ttcaaccata
     1381 aataagtgaa gtaacttcat catatttcta ccaaaaataa tcgggtgcga gagagatcac
     1441 aaagtgtctt atttccggtt actggcgttt atgccctgac tgaactaatt attaatcaac
     1501 ccaataatgt gggtgggtga tagtgtgata acaactctgg agccgtaata tgaaaataat
     1561 ctctaaaatg ttagtcggtg cgttagcgtt tgccgttacc aatgtctatg ccgcagaatt
     1621 aatgaccaaa gcggaatttg aaaaggttgc atcgcaatat gaaaaaatag gtgatatttc
     1681 aaccagtaat gaaatgtcga ccgcagatgc gaaagaagat ttgatcaaaa aagccgatga
     1741 aaaaggggct gatgtgttgg tactgacctc tggtcaaact gacaataaga tccacggcac
     1801 ggcaaatatt tataagaaga agtaattctg aatcctatgt aaacatcccc gatgcgtaag
     1861 tttatcggtg atcatcatct attgaaattt atgccggata aagcgttcgc gctgcattcg
     1921 gcagttcagc ttttcagccg ccgccagaac gtcgtccggc tgatacctaa ataattcgcc
     1981 gctgctgttt tatcgccatt aaatttctcc agtgcctgtt gtgctgtcag caagcctgga
     2041 atgggagtct tcgccgactc gcgtgccagt tccggcagta gcagctgcaa aaattgcggc
     2101 gttaaatccg gcgtcgattc cacacttaaa aacagcgcca gtcgttccat catattgcgc
     2161 agttcacgaa tattgcccgg ccagtcgtag tgcaccagca tggtttcgct ttcctgtaat
     2221 ccctggcgta atgcggcagc aaacggggcg gagagcgccg ccagagacac tttcaaaaaa
     2281 ctttccgcca gcggcagaat atccgccacc cgctcgcgca gtggtggcaa ttgcagacgc
     2341 aaaatactca gccgataaaa cagatcacgg cgaaactgcc cttgccgcat atcttcttcc
     2401 agattgcagt gagtggcgct aatgactcgc acatccaccg gaacaggctg atgcccgccg
     2461 acgcgggtga cctctttttc ttccagcacc cgcagcagcc gagtctgcaa cggcagcggc
     2521 atttcgccta tctcgtcaag aaacagcgtg ccaccgtggg cgatttcgaa cagcccggcg
     2581 cgacctccgc gtcgcgagcc ggtaaacgcc ccttcctcat ggccaaacag ctccgcttcc
     2641 agcagcgatt cggcaatcgc tccgcagttg acggcaacaa acggatgcga ctttttgccc
     2701 tgtcgcgcat cgtggcgggc aaaatattcc cgatgaatcg cctgggccgc cagctctttg
     2761 cccgtccccg tttccccctc aatcaacacc gccgcactgg agcgggcata cagcaaaata
     2821 gtctgccgca cctgttccat ctgtggtgat tgaccgagca tatcgcccag cacgtaacga
     2881 gtacgcaggg cgttgcgggt ggcatcgtga gtgttatggc gtaacgacat gcgcgtcata
     2941 tccagcgcat cgctgaatgc ctggcgcacg gtggcggcgg aatagataaa aattccggtc
     3001 attccggctt cttctgccag atcggtaatc agccccgcgc cgaccaccgc ttcggtgccg
     3061 ttagctttca gctcgttaat ctgcccgcgc gcgtcttctt cggtaatgta gctacgttga
     3121 tcgaggcgca aattaaaggt tttttgaaac gccaccagtg ccggaatagt ttcctgataa
     3181 gtgacaacgc cgatagaaga ggtgagcttt ccggcttttg ccagagcctg taacacatcg
     3241 tagccgctcg gtttaatcaa aataaccggc actgacaggc gacttttaag atatgcgccg
     3301 ttagagccag cagcgatgat ggcgtcgcag cgttcgtttg ccagtttttt gcggatgtag
     3361 gtcaccgctt tttcaaagcc aagctgaata ggggtgatat tcgccaggtg atcaaactcg
     3421 agactgatat cgcgaaacag ctcgaacagc cgcgttacag ataccgtcca gatcaccggt
     3481 ttgtcgtcat tcagccgtgg tggatgtgcc atagcgcacc gcaaagttaa gaaaccgaat
     3541 attgggttta gtcttgtttc ataattgttg caatgaaacg cggtgaaaca ttgcctgaaa
     3601 cgttaactga aacgcatatt tgcgaattac ttcatgacta tattgctaac aaattgaaat
     3661 taaacattta attttattca gtcaattgtg gcacacccct tgctttgtgt ttatcaacgc
     3721 aaataacaag ttgataacaa aggatgggct atgtatctac actctccagg taaagcgttt
     3781 cgcgctgcac tgtctaaaga aaatccattg cagattgttg gcaccatcaa cgctaaccat
     3841 gcgctgctgg cgcagcgtgc cggatatcag gcaatttatc tctccggcgg tggcgtggcg
     3901 gcagggtcgc tggggctgcc cgatctcggt atttctactc ttgatgacgt gctgacagat
     3961 attcgccgta tcaccgacgt ttgttcgctg ccgctgctgg tggatgcgga tatcggtttt
     4021 ggttcttctg cttttaacgt ggcgcgcacc gtgaaatcga tgattaaagc cggtgcggca
     4081 ggattgcata ttgaagatca ggttggtgcg aaacgctgcg gtcatcgtcc gaataaagcg
     4141 atcgtctcga aagaagagat ggtggatcgg atccgcgcgg cggtggatgc gaaaaccgat
     4201 cctgattttg tgatcatggc gcgcaccgat gcgctggcgg tagaggggct ggatgcggcg
     4261 attgagcgtg cgcaggccta tgttgaagcg ggtgccgaga tgttgttccc ggaggcaatt
     4321 accgaactcg ccatgtaccg ccagtttgcc gatgcggtgc aggtgccgat cctcgccaac
     4381 atcaccgaat ttggcgcaac accgctgttt accaccgacg aattacgcag cgcccatgtc
     4441 gcaatggcgc tgtacccact ttcagcgttc cgcgccatga accgcgccgc tgagcatgtc
     4501 tacaacgtcc tgcgtcagga agggacgcag aaaagcgtca tcgacaccat gcagacccgc
     4561 aacgagctgt acgaaagcat caactactac cagtacgaag agaagctcga cgacctgttt
     4621 gcccgtggtc aggtgaaata aaaacgcccg ttggttgtat tcgacaaccg atgcctgatg
     4681 cgccgctgac gcgacttatc aggcctacga ggtgaactga actgtaggtc ggataagacg
     4741 catagcgtcg catccgacaa caatctcgac cctacaaatg ataacaatga cgaggataat
     4801 atgagcgaca caacgatcct gcaaaacagt acccatgtca ttaaaccgaa aaaatcggtg
     4861 gcgctttctg gcgttccggc gggcaatacg gcgctctgca ccgtgggtaa aagcggcaat
     4921 gatttgcatt accgcggcta cgacattctt gatctggcgg aacattgcga atttgaagaa
     4981 gtggcgcatc tgctgatcca cggcaaactg ccgacccgtg acgaactcgc cgcctacaaa
     5041 acgaaactga aagccctgcg cggtttaccg gctaacgtgc gtaccgtgct ggaagcctta
     5101 ccggcggcgt cacacccgat ggatgttatg cgcaccggcg tttccgcgct cggctgcacg
     5161 ctgccagaaa aagaggggca taccgtctct ggcgcgcggg atattgccga caaactgctg
     5221 gcgtcactta gctcgattct tctctactgg tatcactaca gccacaacgg cgaacgcatt
     5281 cagccggaaa ccgatgacga ctccatcggc ggtcacttcc tgcatctgct gcacggcgaa
     5341 aagccgtcgc aaagctggga aaaggcgatg catatttcgc tggtgctgta cgccgaacac
     5401 gagtttaacg cctccacctt taccagccgg gtgattgcgg gcaccggctc tgatatgtat
     5461 tccgcgatta ttggcgcgat tggtgcactg cgcgggccga agcacggcgg ggcgaatgaa
     5521 gtgtcgctgg agatccagca acgctacgaa acgccggacg aagccgaagc cgatatccgc
     5581 aagcgggtgg aaaacaaaga agtggtcatt ggttttggtc atccggttta taccatcgcc
     5641 gacccgcgtc atcaggtgat caaacgtgtg gcgaagcagc tctcgcagga aggcggctcg
     5701 ctgaagatgt acaacatcgc cgatcgcctg gaaacggtga tgtgggagag caaaaagatg
     5761 ttccccaatc tcgactggtt ctccgctgtt tcctacaaca tgatgggtgt tcccaccgag
     5821 atgttcacac cactgtttgt tatcgcccgc gtcactggct gggcggcgca cattatcgaa
     5881 caacgtcagg acaacaaaat tatccgtcct tccgccaatt atgttggacc ggaagaccgc
     5941 cagtttgtcg cgctggataa gcgccagtaa acctctacga ataacaataa ggaaacgtac
     6001 ccaatgtcag ctcaaatcaa caacatccgc ccggaatttg atcgtgaaat cgttgatatc
     6061 gtcgattacg tgatgaacta cgaaatcagc tccaaagtag cctacgacac cgcacattac
     6121 tgcctgctcg acacgctcgg ctgcggtctt gaagctctcg aatacccagc ctgtaaaaaa
     6181 ctgctggggc caattgtccc cggcaccgtc gtacctaacg gcgtgcgcgt ccccggaact
     6241 cagttccagc tcgaccccgt ccaggcggca tttaacatcg gcgcgatgat ccgttggctc
     6301 gatttcaacg atacctggct ggcggcggag tggggccatc cttccgacaa cctcggcggc
     6361 attctggcaa cggcagactg gctttcgcgc aacgcggtcg ctagcggcaa agcgccgttg
     6421 accatgaagc aggtgctgac cgcaatgatc aaagcccatg aaattcaggg ctgcatcgcg
     6481 ctggaaaact cctttaaccg cgtcggcctc gaccacgttc tgttagtgaa agtggcttcc
     6541 accgccgtgg tcgccgaaat gctcggcctg acccgcgagg aaattctcaa cgccgtttcg
     6601 ctggcgtggg tggacggtca gtcgctgcgc acctatcgcc atgcgccgaa caccggcacg
     6661 cgtaaatcct gggcggcggg cgatgccact tcccgcgcgg tacgtctggc actgatggcg
     6721 aaaacgggcg aaatgggtta tccgtcagcc ctgactgcgc cagtatgggg tttctacgac
     6781 gtctccttta aaggtgagtc attccgcttc cagcgtccgt acggttccta cgtcatggaa
     6841 aatgtgctgt tcaaaatctc cttcccggcg gagttccact cccagacggc agttgaagcg
     6901 gcgatgacgc tctatgaaca gatgcaggca gcaggcaaaa cggcggcaga tatcgaaaaa
     6961 gtgaccattc gcacccacga agcctgtatt cgcatcatcg acaaaaaagg gccgctcaat
     7021 aacccggcag accgcgacca ctgcattcag tacatggtgg cgatcccgct gctgttcgga
     7081 cgcttaacgg cggcagatta cgaggacaac gttgcgcaag ataaacgcat cgacgccctg
     7141 cgcgagaaga tcaattgctt tgaagatccg gcgtttaccg ctgactacca cgacccggaa
     7201 aaacgcgcca tcgccaatgc cataaccctt gagttcaccg acggcacacg atttgaagaa
     7261 gtggtggtgg agtacccaat tggtcatgct cgccgccgtc aggatggcat tccgaagctg
     7321 gtcgataaat tcaaaatcaa tctcgcgcgc cagttcccga ctcgccagca gcagcgcatt
     7381 ctggaggttt ctctcgacag aactcgcctg gaacagatgc cggtcaatga gtatctcgac
     7441 ctgtacgtca tttaagtaaa cggcggtaag gcgtaagttc aacaggagag cattatgtct
     7501 tttagcgaat tttatcagcg ttcgattaac gaaccggaga agttctgggc cgagcaggcc
     7561 cggcgtattg actggcagac gccctttacg caaacgctcg accacagcaa cccgccgttt
     7621 gcccgttggt tttgtgaagg ccgaaccaac ttgtgtcaca acgctatcga tcgctggctg
     7681 gagaaacagc cagaggcgct ggcattgatt gccgtctctt cggaaacaga ggaagagcgt
     7741 acctttacct tccgccagtt acatgacgaa gtgaatgcgg tggcgtcaat gctgcgctca
     7801 ctgggcgtgc agcgtggcga tcgggtgctg gtgtatatgc cgatgattgc cgaagcgcat
     7861 attaccctgc tggcctgcgc gcgcattggt gctattcact cggtggtgtt tgggggattt
     7921 gcttcgcaca gcgtggcaac gcgaattgat gacgctaaac cggtgctgat tgtctcggct
     7981 gatgccgggg cgcgcggcgg taaaatcatt ccctataaaa aattgctcga cgatgcgata
     8041 agtcaggcac agcatcagcc gcgtcacgtt ttactggtgg atcgcgggct ggcgaaaatg
     8101 gcacgcgtca gcgggcggga tgtcgatttc gcgtcgttgc gccatcaaca catcggcgcg
     8161 cgggtaccgg tggcgtggct ggaatccaac gaaacctcct gcattctcta cacctccggc
     8221 acgaccggca aacctaaagg cgtgcaacgt gacgttggcg gatatgcggt ggcgctggcg
     8281 acctcgatgg acaccatttt tggcggcaaa gcgggcggtg tgttcttttg tgcttcggat
     8341 atcggctggg tggtggggca ttcgtatatc gtctacgcgc cgctgctggc ggggatggcg
     8401 actatcgttt acgaaggatt accgacctgg ccggactgcg gcgtgtggtg gaaaatcgtc
     8461 gagaaatatc aggttagccg catgttctca gcgccgaccg ccattcgcgt gctgaaaaaa
     8521 ttccctaccg ctgaaattcg caaacacgat ctctcgtcgc tggaagtgct ctatctggcg
     8581 ggggagccgc tggacgagcc aaccgccagt tgggtgagca atacgctgga tgtgccggtg
     8641 atcgacaatt actggcagac cgaatccggc tggccgatta tggcgattgc tcgcggtctg
     8701 gacgacaggc cgacgcgtct gggaagcccc ggcgtgccga tgtatggcta taacgtgcag
     8761 ttgctcaatg aagtcaccgg cgaaccgtgc ggcgtcaacg agaaggggat gctggtggtg
     8821 gaagggccgc tgccgccggg gtgtattcag accatctggg gcgatgacga ccgctttgtg
     8881 aagacgtact ggtcgctgtt ttcccgtccg gtgtatgcca cctttgactg gggcatccgc
     8941 gatgctgacg gttatcactt tattctcggg cgcactgacg atgtgattaa cgttgccggg
     9001 catcggctgg ggacgcgcga gattgaagag agtatctcca gtcatccggg cgttgccgaa
     9061 gtggcggtgg ttggggtgaa agatgcgctg aaagggcagg tggcggtggc gtttgtcatt
     9121 ccgaaagaga gcgacagtct ggaagaccgc gaggtggcgc actcgcaaga gaaggcgatt
     9181 atggcgctgg tggacagcca gattggcaac tttggccgcc cggcgcacgt ctggtttgtc
     9241 tcgcaattgc cgaaaacgcg atccggaaaa atgctgcgcc gcacgatcca ggcgatttgc
     9301 gaaggacgcg atcctgggga tctgacgacc attgatgatc cggcgtcgtt ggatcagatc
     9361 cgccaggcga tggaagagta ggtcggataa ggcgctcgcg ccgcatccga caccgtgcgc
     9421 agatgcctga tgcgacgctg acgcgtctta tcatgcctac
//
LOCUS       DAZFLD010000179         9457 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.180, whole genome shotgun sequence.
ACCESSION   DAZFLD010000179 DAZFLD010000000
VERSION     DAZFLD010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9457
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.180"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..413
                     /locus_tag="ACHKBL_003815"
     CDS             <1..413
                     /locus_tag="ACHKBL_003815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001520089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Nmad5 family putative nucleotide modification
                     protein"
                     /protein_id="HFO5820620.1"
                     /translation="SSLIRKDCCIYLNLAGARANIYFNGNYRGYESGAPEHINKIAPA
                     EFTLLGEDPLVTEFYSFDALYTQIKSDETEIIQNVSAALNKVRTVKQLLDVWPEAKEL
                     LPTDAPPVPLAPAVHRETLNEMIGLPTNTVSDTQ"
     gene            complement(407..1096)
                     /locus_tag="ACHKBL_003816"
     CDS             complement(407..1096)
                     /locus_tag="ACHKBL_003816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000215799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5820621.1"
                     /translation="MTVGNKRQPNGLPEYYCHRGNDIKDAIVRDFIIQPVAHLKLITG
                     QTKTSYSGYPLTDQYYCFSFKKRSDENCTGAFFCGRDVAKDFIRRIGCTELPLFNPMK
                     SDTSAAIERSGSGSGSSGITVKPNADKKLFIDAARLLMTLWDSTSKGQTPLASVLNEA
                     ISGGIDKPPEIRHVVAVNTMLGNTKNTLTGLLDKARVKYPDVLFRDFDFTPLNEIVKR
                     SKSYNKHPPCY"
     gene            1158..3221
                     /locus_tag="ACHKBL_003817"
     CDS             1158..3221
                     /locus_tag="ACHKBL_003817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HFO5820622.1"
                     /translation="MSNILWGDLETYCDIPINNGTHAYAEGVEVMLFAWAINDGPVNV
                     WDITAGGGIPHGLYEAIAAPETLLYFHNSHFDRTVLRYAMPRLAPPVERWRDTMVQAL
                     AHGLPGSLGELCEVLGVPQDKAKDKEGKALIQLFCKPRPKNSKLRRATSKTHPEEWRR
                     FVAYAGLDIEAMREVYKRLPKWNYQGTELALWHRDQQINDRGVCMDMQLARAAIEAVD
                     QEQKRLAKRTQEMTDGEVQAATQRDALIKHIVESYGVELPDMQRSTLERRIADPDLPS
                     AVKELLAIRLQASTTSTSKYKALMKGVSHDGRLRGTLQFCGASRTGRWAGRLFQPQNL
                     PRPSLKQEQIDEGIEALKAGCADLLFDNIMELTSSALRGCIIAPTGKKLVVSDLSNIE
                     GRMLAWLAGEEWKLNAFREYDAGTGPDLYKLAYAKAFDITPDDVDKHMRQIGKVMELG
                     LGYGGGVSAFITFALVYGLDLDELANAALPNIPRDVIREAKSWYNESVKRKSTFGLSE
                     RVFIACDSLKRLWRRAHPATCDFWYELERTVRTAIATPQKTLYCGYLKIRRDGAWLRI
                     QLPSGRAVCYPSPVIEKGNITYMGVNSYSRKWQRLKTYGGKLVENVTQAAARDVLAGN
                     MPLIEDAGYSIVLTVHDEVITEAPDTEDFNDKALSALLSTNPEWAPDIPLNAGGFEAY
                     HYRKD"
     gene            3227..3430
                     /locus_tag="ACHKBL_003818"
     CDS             3227..3430
                     /locus_tag="ACHKBL_003818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5820623.1"
                     /translation="MTVIKTHTGTVITRTGEKQVQLHQNATTWVAGPKEYYYKDTGRR
                     GGAVGVRTRLVLSSIRPIEAKQE"
     gene            3436..3657
                     /locus_tag="ACHKBL_003819"
     CDS             3436..3657
                     /locus_tag="ACHKBL_003819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000008824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HFO5820624.1"
                     /translation="MSFKYRDSPLYYRAAREALRLEQSGEYDRAAKVWAKANRQSRNE
                     LNQDWSERRSDFCLMQNMREKRKAVSDDL"
     gene            3647..4129
                     /locus_tag="ACHKBL_003820"
     CDS             3647..4129
                     /locus_tag="ACHKBL_003820"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000128190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFO5820625.1"
                     /translation="MTCEQTILDMCCGSRMFWFDKEDKRAVFSDIRAEQHELCDGRQL
                     VISPDLIADFRALPFADNTFPVVVFDPPHLERVGDNAWMRKKYGRLNKETWRDDLRDG
                     FAEAFRVLRPHGVLIFKWNETQIPVSNILALTDEKPAIWQRTGKADKTHWVIFVKGVE
                     "
     gene            4129..4398
                     /locus_tag="ACHKBL_003821"
     CDS             4129..4398
                     /locus_tag="ACHKBL_003821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000312939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VRR-NUC domain-containing protein"
                     /protein_id="HFO5820626.1"
                     /translation="MAYERESLIEKHLVAEVKKAGGVAFKFVSPGRRSVPDRIVLLPG
                     GRIVFVECKAPGKPPRPDQLREHERLRKLGFTVVVLDSKNLEGIL"
     gene            4395..4571
                     /locus_tag="ACHKBL_003822"
     CDS             4395..4571
                     /locus_tag="ACHKBL_003822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001367526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HFO5820627.1"
                     /translation="MSKRDDPQLRVRIPQDLKDTLEKISRENDRTLTAEITRRLRKSL
                     EDESTIHSQDLATS"
     gene            complement(4540..4869)
                     /locus_tag="ACHKBL_003823"
     CDS             complement(4540..4869)
                     /locus_tag="ACHKBL_003823"
                     /inference="COORDINATES: protein motif:HMM:NF015807.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HFO5820628.1"
                     /translation="MSKRDDPQLRVRIPELLKEALEKKARANKRTLTSEIVSRLESTI
                     LQDKLLHTSRGFEEAADEILILRDLLEKLKTTYHREYQVEWVYENKYELIEVMEKLKK
                     LLNPENE"
     gene            4982..5158
                     /locus_tag="ACHKBL_003824"
     CDS             4982..5158
                     /locus_tag="ACHKBL_003824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5820629.1"
                     /translation="MAIQITQLHQADIFGYIADMLETARLLSSLEKGEQLAFELIYFA
                     QQAAREAANKPWDE"
     gene            5219..5734
                     /locus_tag="ACHKBL_003825"
     CDS             5219..5734
                     /locus_tag="ACHKBL_003825"
                     /inference="COORDINATES: protein motif:HMM:NF021206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFO5820630.1"
                     /translation="MFETTLTLAGTGKQPMMSSREIAELTSKEHKIVVRDIKALLEQL
                     GFHSTEMYHHENKDFLVKRKIYNGREVIDEILLDQDLSTTLVTGYSAHDRYKVVKRWR
                     ELETGKVQPLVTHPSTPKIQINDDIVRLARVVAEATMKACLDATGYSQPAAPANLSHV
                     EGEYVPVSKAA"
     gene            5997..7391
                     /locus_tag="ACHKBL_003826"
     CDS             5997..7391
                     /locus_tag="ACHKBL_003826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2-related protein"
                     /protein_id="HFO5820631.1"
                     /translation="MGQIFTPRPYQDLIINHQIETLRGNIWAGMGMGKTVATLTALED
                     LFMAGAETRPALVLAPLRVAASTWPDEALKWGHLRNIEVQPIVGNAKARSAALANSNA
                     SVFTINYDNLVWLVEELGERWPFGTVIPDESTRLKSFRLRGGGKRAAALGKVAHKYVQ
                     RWINLTGTPAPNGLVDLWGQLWFVDQGQRLGRTYGAFTSRWFNSIQFPGQSWTKLEPF
                     AHSQAEIQRALADVTLSLDAADWFDIKDPIHNVIRVDMPPKARQQYREMEKEMFLELN
                     GEGIEAPNAAAKTLKCLQIASGAVYTDDTGSWSELHDTKLQALDSILTEAAGAPVLVA
                     YHWKHDLERLLKAFPRGRHLDQDPQTLRDWNSGKIPVLFAHPASAGHGLNMQDGGNIL
                     VFFSHWWDLEQYQQIIERIGPTRQIQAGHNRPVFIHHIIAADTMDEMVMERRNSKRTV
                     QDILLDAMKKRGIA"
     gene            complement(7585..8982)
                     /locus_tag="ACHKBL_003827"
     CDS             complement(7585..8982)
                     /locus_tag="ACHKBL_003827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HFO5820632.1"
                     /translation="MPRTVTHNPDSPNNDDVLAASEKWDACKPPYTSAHMKICVAAAK
                     IILAASGVARRSKYEKENYLRIDFSKAGKVTFYAEFPKKMGLKGKKLGEWPELAIQLA
                     REKALGMADCGLRAESVHAALEMYRDDLKAKVARQKLSPDSFTTYGVRIDRIKATFGE
                     REVFSDVTYNRLVEVLDEWIATRSNNNALELFAELRRFWKFCAPTLCNGRNVAASLPD
                     DYVSSRVQKPTPTRLFTDIESIARLWLNVAACTSVHQKNAVRFMIITGVRPINVHNLR
                     WDYVHEEAGEIVYPEGVIGMRGAMKTQKAFRLPITPEIRRIIDEQKAWRDSVPECNRD
                     YVLLQPRDPMQPFSKRSLDKLVKTYSPDGAVKGIKHDGTVKGKDGAFNTMCRKFLKSN
                     VIALMKERGYSRSDRREISLLCLHHSSKSDDPMAEHYDFSDEILQEEIALKREAFEAH
                     ERSILAQVALLRRRG"
     gene            9197..9457
                     /gene="yfhL"
                     /locus_tag="ACHKBL_003828"
     CDS             9197..9457
                     /gene="yfhL"
                     /locus_tag="ACHKBL_003828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster ferredoxin YfhL"
                     /protein_id="HFO5820633.1"
                     /translation="MSLLITKRCINCDMCEPECPNEAISMGDHIYEINSDKCTECVGH
                     YETPTCQKVCPIPNTIVKDPQHVETEEQLWDKFVLMHHADKI"
BASE COUNT         2534 a         2373 c         2339 g         2211 t
ORIGIN      
        1 atagttcttt aattcgaaaa gattgctgca tatatctcaa tctggctgga gccagagcaa
       61 acatttattt caatggcaac tacagaggtt atgaatcagg agcccccgag catattaata
      121 aaatagcacc tgcagaattt actctacttg gcgaagatcc tctggtaacg gagttttata
      181 gctttgatgc tctctacaca caaattaaaa gtgatgaaac cgaaatcata cagaacgtca
      241 gcgcggctct aaataaagtt cggacagtaa aacaattact ggatgtatgg cccgaagcta
      301 aagaactgct tccaacagac gcaccgccag tccctttagc accagcagta catcgagaga
      361 cattaaatga aatgataggc cttcctacca atacagtgtc tgatactcaa taacaagggg
      421 gatgcttatt atatgatttt gaacgtttta caatctcgtt aagaggcgtg aaatcaaaat
      481 ctctgaataa cacatcagga tacttcactc ttgctttatc cagcaggcca gttaaagtat
      541 tttttgtgtt acccaacatt gtattaaccg ccactacatg tctaatttct ggaggtttat
      601 ctataccacc agaaattgct tcatttagaa ccgacgccag tggggtttgt cccttacttg
      661 ttgaatccca cagcgtcatt aacagacgag ctgcgtctat aaatagcttt ttgtcagcat
      721 ttggcttgac agtaatacca gaactacctg agccagaacc ggaacgttct attgctgcac
      781 tggtatccga tttcatagga ttaaataacg gcagctctgt acaaccgata cgccgaatga
      841 aatcctttgc aacatctcta ccacagaaaa aagcccctgt acaattttca tcagagcgtt
      901 ttttaaatga aaaacaatag tactgatcgg ttaacggata accggaataa ctggttttag
      961 tttgtccagt aataagtttc aggtgtgcaa caggctgaat aataaaatcc cgaactatcg
     1021 catcttttat gtcgttacca cggtgacaat aatattcagg taatccatta ggctgtctct
     1081 tattacccac agtcattagt tatctccttt aattcatgat atctagcatt taaccatata
     1141 ccatatgagg atattttatg tccaatatac tatggggcga cctggaaacc tattgtgaca
     1201 ttccgatcaa caacggtacc catgcgtatg ccgaaggcgt tgaagtgatg cttttcgcat
     1261 gggctatcaa cgacgggcca gtaaacgtgt gggatatcac tgccggtggt ggtattccac
     1321 acggcttata cgaagcaatc gcagcccctg aaaccctgct ttatttccat aactctcact
     1381 tcgaccgcac cgttctgcgt tatgcaatgc cgcgactggc accgccagtc gaacgttggc
     1441 gcgacacgat ggttcaggcg ctggcgcacg gtctcccggg gtctctgggg gaactctgcg
     1501 aagtactagg cgtcccgcaa gacaaagcga aggacaaaga aggtaaagcg ctgatccagc
     1561 tgttctgtaa gccacgcccg aaaaacagca aactgcgtcg ggccaccagt aaaacccacc
     1621 cggaagaatg gcggcgcttt gttgcttacg ccggactgga tatcgaggca atgcgcgaag
     1681 tctataaacg tctgccgaag tggaattatc aggggacaga gctggcgctc tggcatcgtg
     1741 atcagcagat caacgaccgg ggcgtctgca tggacatgca actcgcgcgc gctgcgatcg
     1801 aagcggtaga ccaagagcaa aagcgcttgg caaagcgtac acaggaaatg actgatggcg
     1861 aagtgcaggc agccacacaa cgggacgcgt tgattaagca catcgttgaa tcctacggtg
     1921 tggagctacc agacatgcaa cgcagtaccc tggaacgtcg tatagccgac cccgatttac
     1981 catctgccgt gaaagaactg ctggctatcc gcctgcaggc cagtactacc agcaccagta
     2041 aatacaaggc actgatgaaa ggcgtaagcc acgacgggcg cttacgcggt acgctacagt
     2101 tctgcggagc gtcacgtacc ggtcgttggg ccggacggct attccagccc cagaaccttc
     2161 cccgtccttc actaaaacag gaacaaatag acgaaggcat cgaagcactg aaagccggat
     2221 gtgcagacct gctgtttgac aatatcatgg aactaaccag ttcagcgtta cgtggctgca
     2281 ttatcgcgcc aacaggcaaa aaactggtgg taagtgactt gtcaaacatt gaaggccgta
     2341 tgctggcatg gctggcggga gaagaatgga aactgaatgc attcagagag tacgacgccg
     2401 gaacgggtcc ggacttatat aaactggcgt atgcaaaagc tttcgatatt acaccagatg
     2461 atgttgataa acacatgcgt cagatcggta aagtcatgga actcggtctg ggttatggag
     2521 gtggtgtatc ggctttcatc acttttgctc tggtttacgg tctcgatctc gacgagctgg
     2581 cgaacgccgc actgccaaac attccccgcg atgttatccg cgaggcgaaa agctggtaca
     2641 acgaatcggt taaacgtaag tcgacctttg gcctgtccga gcgtgtattc atcgcatgtg
     2701 actcgctcaa gcgcctgtgg cgcagggcgc acccggcgac ctgcgatttc tggtacgaac
     2761 tggagcgcac cgtccgcact gcaatcgcca caccgcaaaa aacattgtat tgcggttatc
     2821 ttaaaatccg ccgcgatggc gcgtggctgc gcatacagct accatccgga cgcgctgtat
     2881 gctacccctc tccggttatc gaaaaaggga atatcaccta tatgggtgtt aactcttatt
     2941 cgcgtaaatg gcaacgactc aaaacctacg gcggaaagct ggtagagaac gtcacccagg
     3001 cggccgcccg cgacgttctg gccggaaaca tgccgcttat cgaagatgcc ggttacagca
     3061 ttgtgctgac ggtacacgac gaggtgatca ccgaagcacc tgacacagaa gatttcaacg
     3121 ataaagcgct ttccgcgctt ctctccacta accccgaatg ggcgcccgat atcccactga
     3181 acgctggcgg ttttgaggcg taccactacc gtaaggatta atcgctatga ctgtaatcaa
     3241 aacgcatacc ggaacggtta ttacccgcac cggtgaaaag caggtgcaat tgcatcaaaa
     3301 cgcgacaacg tgggtggctg gcccaaaaga gtattactac aaagacactg gccgccgcgg
     3361 tggtgctgtc ggtgtccgca ctcgtctggt gctgagcagc atccgcccaa tcgaagcaaa
     3421 gcaggagtaa cccctatgtc atttaaatat cgggacagtc cgctttatta ccgggctgcg
     3481 agggaggcct tgcgccttga gcagtccggc gaatatgacc gggcagcgaa ggtctgggcc
     3541 aaggctaacc gccagtcacg taacgaactg aatcaggact ggagcgaacg ccggtctgat
     3601 ttttgtctga tgcagaacat gcgcgaaaag cgtaaggcgg tgagcgatga cttgtgagca
     3661 gaccattctt gacatgtgct gcggctctcg tatgttttgg ttcgacaagg aggacaagcg
     3721 cgccgtgttc agtgatatcc gcgccgagca gcatgagctt tgcgacggtc gccagttggt
     3781 tataagtccg gaccttatag ctgatttccg cgcccttccc tttgccgata acactttccc
     3841 tgtcgtcgtt ttcgatccgc cgcacctcga gcgcgtcggc gataacgcat ggatgaggaa
     3901 aaagtacggg cggctcaaca aagaaacatg gcgcgatgat cttcgcgacg gcttcgccga
     3961 agcattcagg gtattgcggc cccacggtgt actcatattc aaatggaacg aaacgcagat
     4021 cccggtaagc aatatcttgg cgctgaccga cgagaagccg gccatatggc aacgaaccgg
     4081 aaaagccgac aaaacccact gggttatttt cgtcaaaggt gtggaatgat ggcctacgaa
     4141 cgtgaaagcc ttatcgaaaa gcacctcgtc gccgaagtga aaaaggctgg cggggtcgcc
     4201 tttaagttcg tgtcacccgg tcgccgctcg gtaccggatc gcattgtcct gctacccggc
     4261 ggtcgcatcg tctttgtcga atgcaaagcg ccaggcaagc caccacggcc tgaccagttg
     4321 cgcgagcatg aacgtctgcg aaagctgggc tttaccgtgg tggtgctgga tagcaaaaat
     4381 ctggagggaa tattgtgagt aaacgtgatg acccacaatt gcgggtacgc atcccgcaag
     4441 acttgaaaga cacactggaa aaaatttcca gagaaaacga cagaacacta accgccgaaa
     4501 tcacccggcg gttacgtaaa agtttagagg atgaatccac tattcattct caggatttag
     4561 caacttctta agtttttcca tcacttcaat aagctcgtat ttgttttcat aaacccattc
     4621 gacctgatac tctcgatggt acgttgtctt aagtttctcg agaagatccc tgagtatgag
     4681 aatctcatca gcagcttcct caaaccccct agatgtatga agtaatttgt cctgaagaat
     4741 agtagattcc aaccggctaa cgatctcaga ggtcagtgtt ctcttattag cccgtgcttt
     4801 cttctccaaa gcctctttta gcaattctgg tatgcgtacc ctcaactgcg ggtcgtctct
     4861 tttgctcata tatgatccct tgaaagtgtg agaacaacat gcctcacaaa catgttgact
     4921 tcaagcctca ttgtgagtac taataaagtc ctcacaatga gtacaaaaca aggttaacgc
     4981 tatggctatt cagatcacgc agttacatca ggcagatatt ttcgggtata tcgcggacat
     5041 gctggaaaca gcgagacttc tcagttcgct ggaaaaagga gagcagttag cttttgaatt
     5101 gatttatttt gcgcagcagg ccgcaaggga agctgcaaac aagccgtggg atgaataaac
     5161 aaaacgcgct gatagttgca gctaccagcg catcttttac tgaatggaga atacttcaat
     5221 gttcgaaaca actttaacac tagcaggcac tggtaagcaa ccgatgatga gcagtcgcga
     5281 gattgcagaa cttaccagca aagaacataa aatagtcgtg cgcgatatta aagcacttct
     5341 tgagcagttg ggttttcata gtacagaaat gtaccatcat gaaaataaag actttttagt
     5401 caaaaggaag atctataatg gtcgcgaagt cattgatgaa atactactcg atcaggacct
     5461 ttccacaacg cttgtaactg gttatagcgc ccacgaccgg tataaagtag ttaagcgctg
     5521 gcgtgagctg gaaacaggta aagtacaacc gcttgttacc catccttcca cacccaaaat
     5581 tcagatcaac gacgacatcg tccggcttgc tcgcgtcgta gcagaagcaa caatgaaagc
     5641 ctgtcttgat gcaactggct attctcaacc agccgcacca gcaaatttat ctcatgttga
     5701 aggtgaatat gtccccgtca gcaaagcagc atagaaaaca ggtttatctg attcaacatg
     5761 ccggaaattg atcggcttcg ccaggatacc ggtacgttca gacaccggtg ttcggggatt
     5821 gctcgttaac ctcccagcga tcgaacaagc agctcaaaag ctgttaaacg aatcgacacc
     5881 gccgaaaggt aaccgcaagc gctggagaca tccgcagttt ggcaacttca cctactacac
     5941 cgatctaatc tgacattatt gcgcgggatt acccgcgccc tactggctaa aacactatgg
     6001 gacaaatttt caccccccgc ccgtatcaag acctgatcat caatcaccaa atcgaaaccc
     6061 tgcgcggcaa catctgggcg gggatgggaa tgggtaaaac cgtggcaacg ctcactgcgc
     6121 tggaagacct ctttatggca ggggcagaaa cacgccccgc gctggtcctc gcgccgctac
     6181 gcgttgcagc aagcacatgg ccggatgaag cgctgaaatg ggggcatctg cgcaatatcg
     6241 aggtgcagcc gattgttggt aacgccaaag cgcgctctgc agcgctggcg aacagcaacg
     6301 cgagcgtgtt caccatcaac tacgataacc ttgtctggct ggttgaggaa ttgggagaac
     6361 gatggccgtt cggtactgtc attccagatg aaagcacccg gctaaaatcc ttccggctgc
     6421 gaggtggtgg taagcgcgcg gcggcgctgg gaaaagtggc gcataagtat gtccagcgct
     6481 ggataaatct caccggtacg ccagcaccga acggcctggt agatttgtgg ggacaattgt
     6541 ggtttgtgga ccaggggcaa cgtctcgggc gcacttacgg cgcgtttacc tcacgctggt
     6601 tcaactcgat acagtttccg gggcagagct ggaccaaact ggagccgttt gctcactcac
     6661 aggctgaaat acagcgagcg ttagccgatg tgaccctctc gctggatgcg gccgactggt
     6721 tcgatatcaa agaccccatc cataacgtaa tccgcgtgga tatgccgccg aaggcccgtc
     6781 agcagtatcg tgaaatggaa aaggaaatgt tcctcgagct gaatggcgaa ggcatcgaag
     6841 caccgaacgc cgcggcaaag acactgaagt gtcttcaaat cgccagtggc gcagtataca
     6901 cagacgacac cggaagttgg tcagaactgc atgacaccaa actacaggcg ctggacagca
     6961 tactgaccga agcagctggc gcacctgtgc tggttgctta tcactggaaa cacgatcttg
     7021 aacgcttgct taaagcattc cctcgcggtc gtcacctcga ccaagatcca cagacactgc
     7081 gcgactggaa ttccggaaag attcctgttc tctttgcaca cccagccagc gcgggccacg
     7141 gtctgaacat gcaggacggc ggaaacatac tggtattttt ctcacactgg tgggacctgg
     7201 agcagtatca gcaaattatt gaacgtatcg gccccaccag gcagatacag gccggacaca
     7261 atcgtccggt atttattcac cacattattg cggccgacac tatggacgaa atggtgatgg
     7321 agcggcgcaa ctcaaaacga acagtgcagg acatcctgct cgatgccatg aaaaagagag
     7381 gtatagcatg agcgagaaac ccgacgattt actcaccccg gatgaagtat gccaaaagtt
     7441 aggtattaca cagaaaacgc tatgtgagtg gaatattaag catcgtcatc gggctatcct
     7501 ggcaccaatt cgtttcagtg caaaagtagt tcgttatgag cgccgaaatg tcgacgcttt
     7561 tattcaaaag tgtcgcagcc agtattaacc tcgccgccgt agcaatgcca cctgcgcaag
     7621 tatgctccgc tcgtgagcct cgaaagcttc gcgctttaac gcaatctctt cctgcaaaat
     7681 ctcatcagaa aagtcgtaat gttctgccat cgggtcatct gacttgctgg agtggtgaag
     7741 gcaaaggagg ctgatttccc ttcggtctga tcgggaatag cctctttcct tcatcaaggc
     7801 aataacattg ctcttaagga atttacggca catcgtatta aatgcaccgt ctttcccttt
     7861 aacagtccca tcatgtttta ttccttttac agccccgtcc gggctgtatg ttttcaccag
     7921 cttatccagt gatcgttttg aaaatggctg cattggatca cgtggctgca aaagtacata
     7981 atccctgttg cactcaggaa ctgaatcacg ccaggctttc tgctcgtcga taatccgccg
     8041 gatctcaggc gttatcggca ggcggaaagc cttttgtgtt ttcatagccc ctcgcatgcc
     8101 gataacccct tccggataaa caatttcacc agcctcctcg tgaacgtagt cccagcgcag
     8161 gttatggaca ttaatcggac gaacgccagt gatgatcatg aagcgaacag cattcttctg
     8221 gtgtacagag gtgcaggcag caacattgag ccagagtcgg gcgattgatt caatatcggt
     8281 aaaaagccgt gtgggggtag gtttctgtac gcgggaggaa acataatcat ctggcagact
     8341 ggcggcaaca ttgcggccgt tgcaaagagt aggtgcgcag aacttccaga accgacggag
     8401 ctcggcaaac aactccaggg cgttattgtt cgagcgagtg gcgatccact cgtccagcac
     8461 ttccaccagc cgattgtatg ttacgtcgct gaacacctca cgctcgccga acgttgcttt
     8521 aatccggtcg atacgcaccc cgtaggttgt gaaactgtcc gggctcagct tctgccgggc
     8581 gactttggct ttgaggtcat cccggtacat ttccagcgct gcatgtactg actctgcccg
     8641 caagccacag tcagccatac ctagcgcttt ttcgcgcgcc agctggatag cgagctccgg
     8701 ccactcgccg agctttttac ccttgaggcc catctttttt ggaaactcgg cgtaaaatgt
     8761 aaccttaccg gctttgctga aatcgatacg gagatagttc tctttttcgt atttggaacg
     8821 gcgagccacg ccggaagcag cgaggatgat tttggcggca gcaacacaga ttttcatgtg
     8881 tgcgctggta taggggggtt tacaggcgtc ccatttttca gatgcggcta aaacatcgtc
     8941 attattgggg ctatccggat tatgtgttac agtgcgcggc attctcaatc cttatctgcg
     9001 taggcgcaga aaacaagcac acacatacaa gtctttttta cgggacaaaa tgcaatgtgt
     9061 tgcggttttg tgttgctgga ctgagtttat caaggttaaa tacactggat caacatacag
     9121 taagttaatg acagtaaagc atacaaactc gatacaactt actgatttta aaatgattta
     9181 acggtaattc attgaaatgt ctttactaat tactaaacgc tgtattaatt gtgatatgtg
     9241 tgaacccgaa tgcccgaatg aggcgatttc aatgggcgat catatctacg agattaacag
     9301 cgataagtgt accgaatgcg tagggcacta cgagacacca acctgccaga aggtgtgccc
     9361 gatccccaat actattgtga aggatccgca acatgtcgag acggaagaac agttgtggga
     9421 taaatttgtg cttatgcatc acgcggataa aatttaa
//
LOCUS       DAZFLD010000180         9356 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.181, whole genome shotgun sequence.
ACCESSION   DAZFLD010000180 DAZFLD010000000
VERSION     DAZFLD010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9356
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.181"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(6..725)
                     /gene="dcuR"
                     /locus_tag="ACHKBL_003829"
     CDS             complement(6..725)
                     /gene="dcuR"
                     /locus_tag="ACHKBL_003829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator DcuR"
                     /protein_id="HFO5820634.1"
                     /translation="MINVLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEIIF
                     NSDTPIDLILLDIYMQKENGLDLLPVLHNARCKSDVIVISSAADAATIKDSLHYGVVD
                     YLIKPFQASRFEEALTGWRQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLT
                     PQTLRTLCQWIDAHQDYEFSTDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTG
                     RPVYRYRIQAEHYSLLKQYCQ"
     gene            complement(722..2353)
                     /gene="dcuS"
                     /locus_tag="ACHKBL_003830"
     CDS             complement(722..2353)
                     /gene="dcuS"
                     /locus_tag="ACHKBL_003830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFO5820635.1"
                     /translation="MRHSLPYRMLRKRPMKLSTTVILMVSAVLFSVLLVVHLIYFSQI
                     SDMTRDGLANKALAVARTLADSPEIRQGLQKKPQESGIQAIAEAVRKRNDLLFIVVTD
                     MQSLRYSHPEAQRIGQPFKGDDILKALNGEENVAINRGFLAQALRVFTPIYDENHKQI
                     GVVAIGLELSRVTQQINDSRWSIIWSVLFGMLVGLIGTCILVKVLKKILFGLEPYEIS
                     TLFEQRQAMLQSIKEGVVAVDDRGEVTLINDAAQELLNYRKSQDDEKLSTLSHSWSQV
                     VDVSEVLRDGTPRRDEEITIKDRLLLINTVPVRSNGVIIGAISTFRDKTEVRKLMQRL
                     DGLVNYADALRERSHEFMNKLHVILGLLHLKSYKQLEDYILKTANNYQEEIGSLLGKI
                     KSPVIAGFLISKINRATDLGHTLILNSESQLPDSGSEDQVATLITTLGNLIENALEAL
                     GPEPGGEISVTLHYRHGWLHCEVNDDGPGIAPDKIDHIFDKGVSTKGSERGVGLALVK
                     QQVENLGGSIAVESEPGIFTQFFVQIPWDGERSNR"
     gene            2533..2763
                     /gene="yjdI"
                     /locus_tag="ACHKBL_003831"
     CDS             2533..2763
                     /gene="yjdI"
                     /locus_tag="ACHKBL_003831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S mono-cluster protein YjdI"
                     /protein_id="HFO5820636.1"
                     /translation="MDQALLDRGYRCYTGEKIDVYFNTAICQHSGNCVRGNGKLFNLK
                     RKPWIMPDEVDVATVVKVIDTCPSGALKYRHK"
     gene            2775..3047
                     /gene="yjdJ"
                     /locus_tag="ACHKBL_003832"
     CDS             2775..3047
                     /gene="yjdJ"
                     /locus_tag="ACHKBL_003832"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO5820637.1"
                     /translation="MEIREGHNKFYINDEQGKQIAEIVFVPTGENLAIIEHTDVDESL
                     KGQGIGKQLVAKVVEKMRREKRKIIPLCPFAKHEFDKTREYDDIRS"
     gene            3274..3570
                     /gene="ghoS"
                     /locus_tag="ACHKBL_003833"
     CDS             3274..3570
                     /gene="ghoS"
                     /locus_tag="ACHKBL_003833"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HFO5820638.1"
                     /translation="MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMSFSSIVADEY
                     GIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDIDFDLNIMTVDDYFRQFYK"
     gene            3598..3771
                     /gene="ghoT"
                     /locus_tag="ACHKBL_003834"
     CDS             3598..3771
                     /gene="ghoT"
                     /locus_tag="ACHKBL_003834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system toxin GhoT"
                     /protein_id="HFO5820639.1"
                     /translation="MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITW
                     PMSLPVALLFSLF"
     gene            complement(3890..5407)
                     /gene="lysS"
                     /locus_tag="ACHKBL_003835"
     CDS             complement(3890..5407)
                     /gene="lysS"
                     /locus_tag="ACHKBL_003835"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFO5820640.1"
                     /translation="MSERETRGANEAIDFNDELRNRREKLAALRQQGVAFPNDFRRDH
                     TSDQLHEEFDAKDNQELESLNIEVSVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     SLPEGVYNDQFKKWDLGDIIGARGTLFKTQTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEVRYRQRYLDLIANDKSRQTFVVRSKILAAIRQFMVARGFMEVETPMMQVIPGGA
                     SARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYHDLIELTESLFRTLAQEVLGTTKVTYGEHVFDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDAAKALAESIGITVEKSWGLGRIVTEIFDEVAEAHLIQPTFITE
                     YPAEVSPLARRNDVNPEITDRFEFFIGGREIGNGFSELNDAEDQAERFQEQVNAKAAG
                     DDEAMFYDEDYVTALEYGLPPTAGLGIGIDRMIMLFTNSHTIRDVILFPAMRPLK"
     gene            complement(5644..7101)
                     /gene="dtpC"
                     /locus_tag="ACHKBL_003836"
     CDS             complement(5644..7101)
                     /gene="dtpC"
                     /locus_tag="ACHKBL_003836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpC"
                     /protein_id="HFO5820641.1"
                     /translation="MKTPSQPRAIYYIVAIQIWEYFSFYGMRALLILYLTHQLGFDDN
                     HAISLFSAYASLVYVTPILGGWLADRLLGNRTAVIAGALLMTLGHVVLGIDTNSTFSL
                     YLALAIIICGYGLFKSNISCLLGELYDENDHRRDGGFSLLYAAGNIGSIAAPIACGLA
                     AQWYGWHVGFALAGGGMFIGLLIFLSGHRHFQSTRSMDKKALTSVKFALPVWSWLVVM
                     LCLAPVFFTLLLENDWSGYLLAIVCLIAAQIIARMMIKFPEHRRALWQIVLLMFVGTL
                     FWVLAQQGGSTISLFIDRFVNRQAFNIEVPTALFQSVNAIAVMLAGVVLAWLASPESR
                     GNSALRVWLKFAFGLLLMACGFMLLAFDARHAAAGGQASMGVMISGLALMGFAELFID
                     PVAIAQITRLKMSGVLTGIYMLATGAVANWLAGVVAQQTTESQISGMAIAAYQRFFSQ
                     MGEWTLACVAIIVVLAFATRFLFSTPTNMIQESND"
     gene            complement(7160..8017)
                     /gene="cadA"
                     /locus_tag="ACHKBL_003837"
                     /pseudo
     CDS             complement(7160..8017)
                     /gene="cadA"
                     /locus_tag="ACHKBL_003837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313140.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase CadA"
     gene            8083..9245
                     /locus_tag="ACHKBL_003838"
     CDS             join(8083..8344,8344..9245)
                     /locus_tag="ACHKBL_003838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS3 family transposase"
                     /protein_id="HFO5820642.1"
                     /translation="MTKTVSTSKKPRKQHSPEFRSEALKLAERIGVTAAARELSLYES
                     QLYNWRSKQQNQQTSSERELEMSTEIARLKRQLAERDEELAILPKGRDILREAPEMKY
                     VFIEKHQAEFSIKAMCRVLRVARSGWYTWCQRRTRISTRQQFRQHCDSVVLAAFTRSK
                     QRYGAPRLTDELRAQGYPFNVKTVAASLRRQGLRAKASRKFSPVSYRAHGLPVSENLL
                     EQDFYASGPNQKWAGDITYLRTDEGWLYLAVVIDLWSRAVIGWSMSPRMTAQLACDAL
                     QMALWRRKRPRNVIVHTDRGGQYCSADYQAQLKRHNLRGSMSAKGCCYDNACVESFFH
                     SLKVECIHGEHFISREIMRATVFNYIECDYNRWRRHSWCGGLSPEQFENKNLA"
BASE COUNT         2477 a         2270 c         2261 g         2348 t
ORIGIN      
        1 atccgttatt ggcaatattg tttcagcagt gagtagtgtt ctgcctgaat acggtaacgg
       61 taaaccggac gccctgtgac gccataatgg atactggtga acaagatgtg gcagttgacc
      121 agccagatga ggtatttacg gcaggaaaca cgcgagatgt taacctcgtt ggccagctcg
      181 tcggttgaaa actcatagtc ctgatgcgcg tcaatccact ggcacagcgt gcgtaacgtc
      241 tgcggcgtca agccttttgg caagcgacga ggatcctgtt cgttggagct gctgccgtgg
      301 attagctgat caagctcggc ctggtcataa tactgatgtt tttccagcgc cattttcttt
      361 tgccgccagc cggtgagcgc ctcttcaaag cgggaagcct ggaagggttt gatcaggtaa
      421 tccacgacac cgtaatgcag cgaatcttta atggttgccg catcggctgc ggaggagatg
      481 acaatcacat cacttttgca acgcgcgtta tgcaggacag gcagtaaatc gagcccgttc
      541 tctttttgca tatagatatc gagcaatatc aggtcgatag gcgtatcgct attgaagata
      601 atctctttgg ctttctccag cgtcgaagcc gttccacagc attgaaagcc tgggatttgt
      661 gccacgtatc ggcgattcag ctccgcgacc attgcgtcgt catcgataat taatacattg
      721 atcatctgtt cgacctctcc ccgtcccagg gtatctggac aaaaaattgt gtgaaaatcc
      781 cgggttccga ttccacggcg atgctgccgc cgagattttc tacctgttgt ttgacaagtg
      841 ctaaaccgac gcctcgctcg cttccttttg tcgagacacc tttgtcaaaa atgtgatcga
      901 ttttgtcggg tgcgatcccc ggtccatcat cgttaacttc acagtgcagc cagccgtgac
      961 ggtagtgcaa tgttacgcta atttcgccac cgggttccgg tcctaatgcc tccagcgcgt
     1021 tttctatcag atttcccagc gtggtaatca gcgtcgcgac ctggtcttca ctgccgctgt
     1081 ctggcaactg gctttcactg tttaaaatca gcgtatggcc taaatcggtc gcgcggttaa
     1141 tcttactgat taaaaaacca gcgataaccg gagatttgat cttacccagc agagagccaa
     1201 tctcttcttg atagttattg gctgttttga gaatgtaatc ttccaactgc ttataactct
     1261 tcagatgcaa taatccgaga atcacgtgca atttattcat aaattcgtgg gatcgttcac
     1321 gaagtgcgtc agcatagttg accagaccat cgagtcgctg catcagttta cgtacttcag
     1381 ttttgtccct gaaggttgaa atggcaccga tgataacgcc attactgcgc accggaacgg
     1441 tgttgatcag taatagccgg tctttaatcg taatctcttc gtcgcgccgc ggggtaccgt
     1501 cgcgtaacac ttccgagaca tctaccacct gtgaccacga gtggcttagc gtcgacagct
     1561 tctcatcgtc ctgcgactta cggtaattca gcaattcttg tgcggcatcg ttgatcagcg
     1621 tgacttcacc gcgatcgtcc acggcaacga cgccttcttt gatggactgc aacatagcct
     1681 ggcgttgctc aaacagcgtg gagatttcgt agggttccag gccgaaaagg atttttttca
     1741 gtaccttaac cagaatgcag gtgccaatca gcccgaccag catgccaaat aataccgacc
     1801 agatgatgct ccagcgactg tcattgatct gttgggtcac gcgacttaac tcaaggccga
     1861 tcgccaccac gccaatttgt ttatgatttt catcgtagat gggggtaaat acgcgtaaag
     1921 cctgcgccag aaaaccgcga ttgatagcga cattttcttc gccattcagc gctttaagga
     1981 tgtcatcacc tttaaatggc tgaccaatac gctgggcttc aggatgcgag tagcgaagac
     2041 tttgcatatc ggtaacgaca ataaacagca gatcgttgcg tttgcgcacg gcttccgcga
     2101 tggcctggat tccactctcc tgtggttttt tctgcaagcc ctgacggatt tccggcgagt
     2161 cggcgagggt acgcgctact gccagtgcct tgttagctag cccgtctcgc gtcatatcac
     2221 tgatttgcga gaagtaaatc agatgcacca ccaatagcac cgagaacagt accgcgctga
     2281 ccattaagat cactgtggta ctcaatttca tcggacgttt gcgtaacatg cggtagggca
     2341 atgaatgtct catcagcttc cttgtgtgac aaatttctta agcattatct ctgatgaggc
     2401 gggtaattca aagggagtaa gaatgattgg catatagggg aagagactct ggcaacggaa
     2461 actgccagtg ctgtatgaag attccagggc tatgcttata gtgaaaacca tactgatgag
     2521 agagggaagg tcatggatca ggcgctactg gacaggggtt atcgctgtta taccggcgaa
     2581 aagatcgatg tctatttcaa cactgcgata tgtcagcatt ctggcaattg cgtacgtggc
     2641 aacggcaagt tatttaatct caaacgaaag ccgtggatca tgccggatga agtcgacgtc
     2701 gccactgtgg ttaaagtgat tgatacgtgc ccgagcggcg cgctgaaata ccgtcataaa
     2761 taagcgaggg taaaatggaa atacgcgaag gccacaataa attttacatt aatgacgaac
     2821 agggcaagca aatcgctgaa attgtctttg tgccgaccgg agagaattta gcgattatcg
     2881 aacataccga tgtcgatgaa agcctgaaag ggcaagggat tggtaaacag ctggttgcga
     2941 aagtcgtgga aaaaatgcgt cgggaaaaac gaaaaattat cccactatgc ccatttgcga
     3001 aacacgaatt tgataaaacg cgggagtatg atgatattcg cagttgatgg gagagtacag
     3061 agtcacgata tttttcatgc tctccgcggt gtgatgcagg agagcatctg aagggtaggg
     3121 ggatgcacaa agaatgggca gagagcgcgt ttttttgtcc caagtcatcc cctttactga
     3181 gcaaaaaaaa gaatatctcc tatatgagaa tcatcaatcg gggttaataa gttttgcgtc
     3241 cccagagcgt ttaatattga taggagtcat attatggaag gtaaaaacaa gttcaatact
     3301 tatgttgttt cttttgatta tccatcatct tattcctcag tgttcttaag gttaagatca
     3361 ttgatgtatg atatgagttt ctcctctatc gtggctgatg aatatgggat accacgacaa
     3421 ctgaatgaaa actccttcgc aataacgaca tcgttagccg caagtgaaat cgaagattta
     3481 atcaggctta aatgcttaga cttaccggat attgactttg acctcaacat tatgacagtt
     3541 gatgactatt tccgtcagtt ttacaagtag ccaggaagga taagaagaac ataaaaaatg
     3601 gcactattct ctaaaatatt aattttttat gtgattggtg tgaacatatc ctttgtcatt
     3661 atctggttta tctcacatga gaaaacacat attcgtttac ttagtgcatt tctggtcgga
     3721 ataacctggc caatgagtct gcctgtggca ttactttttt ctctctttta ggagctgtag
     3781 ttgagtggac ccgctggtta tcttgaaacg aagcgtctct cctcaaaact ggatttgcgg
     3841 aatgacgaac gaagcagtcg cctgactgct tcgttcatta aagtgaaatt tatttcagtg
     3901 ggcgcatcgc cgggaagaga ataacgtcgc gaatagtatg actgtttgta aacagcataa
     3961 tcattcggtc gataccaata cccagaccag cggttggcgg cagaccatat tccagcgcag
     4021 ttacgtaatc ttcgtcatag aacatggctt cgtcgtcacc tgcagcttta gcgttaacct
     4081 gttcctggaa acgttcagcc tgatcttctg cgtcgtttaa ttcgctaaaa ccattaccaa
     4141 tttcacgacc accgatgaag aattcaaaac ggtcggtgat ttccgggtta acatcattac
     4201 ggcgcgccag cggtgacact tctgccggat attccgtgat aaaggttggc tgaatcagat
     4261 gtgcttctgc cacttcatca aagatctccg tgacaatgcg tcccaacccc cagcttttct
     4321 ctaccgtaat tccgatagat tcagctaatg ctttagcagc atcaaaatta tccagatccg
     4381 ccatatcggt ttctggacga tattttttga ttgcttcgcg catggtgagt ttttcaaacg
     4441 gtttgccgaa atcaaacaca tgctcgccat aagtgacttt agtggtaccc agtacctctt
     4501 gtgccagagt gcggaacagt gattccgtca gttcaatcaa atcgtggtaa tccgcatacg
     4561 ccatgtagag ttccatcatc gtgaactcag gattatgacg aacagaaata ccttcattac
     4621 ggaagttacg gttgatttcg aatacccgtt caaaaccacc tacaaccaga cgtttcagat
     4681 atagctccgg cgcgatacgc aggtacatat ctaaatccag agcgttatga tgggtaataa
     4741 acgggcgagc agatgctcca cctggaatta cctgcatcat cggggtttct acttccataa
     4801 agccgcgagc gaccatgaac tgacggatag cggccagaat ttttgaacgg acaacaaacg
     4861 tttgacggga tttatcgtta gcgatgaggt ccagataacg ttgacgataa cggacttcct
     4921 gatcctgcag accatggaat ttatctggta aaggacgtag tgctttagtc agcaggcgca
     4981 gctcagtaca gtgaatggaa agctcacccg tttgcgtctt aaacagcgta ccgcgggcac
     5041 cgataatgtc acccagatcc cattttttaa actgatcgtt ataaacacct tctggcaggc
     5101 tatctcttgc aacgtacagt tgaatacggc caccgacatc ctgcaacgtt acaaaggagg
     5161 ctttccccat aatacgacgg gtcatcattc ggccagcaac cgagacttca atgtttaagg
     5221 attccagttc ctggttatcc ttcgcatcaa actcttcgtg caactggtca gaggtatggt
     5281 cgcggcgaaa atcattggga aacgccacac cttgctgacg tagtgccgcc agtttttcgc
     5341 ggcgatttct cagttcatcg ttaaaatcaa tagcctcatt ggctccccgt gtttctcgtt
     5401 cagacatttt ggttcctcta aatccagctt tcaatttagc gtagataaag agacagatcg
     5461 gtacactgta ttatctgcct cgactattaa taactcagtt tatcttatcg aaactaacgc
     5521 ttacctcaag ttgatatcac taataaataa ccataaaaat ggtttaaatg acttattaat
     5581 ctcgatttgc tagtgtgata aactgaaagt taatttattc ataaccaatt gtttttacga
     5641 cagttaatcg ttgctctcct gtatcatatt cgtcggcgtg ctaaacagaa aacgggtagc
     5701 aaaggccaat accacgatga tcgcgacaca agccaacgtc cactctccca tctgagaaaa
     5761 gaatcgctgg taagcggcaa ttgccatacc gctaatttgc gactctgtcg tctgctgtgc
     5821 cacgacgcct gccagccagt tggcgaccgc gcctgttgcc agcatataaa taccggttaa
     5881 tacgccagac attttcagac gcgtgatttg cgcaatcgcc accgggtcaa taaagagttc
     5941 agcaaagccc attagcgcca gcccggatat catcacgccc attgacgctt gaccgccagc
     6001 cgctgcatgt cgggcatcaa atgccagcaa cataaagcca caagccatca gcagtaggcc
     6061 aaaggcaaac ttcagccaga cgcgcaatgc tgaattgccg cggctttctg gcgacgccag
     6121 ccaggccaga acaaccccag cgagcatcac cgcaatagca ttcaccgact ggaatagtgc
     6181 tgtaggtact tcaatattga atgcctgacg attcacaaag cgatcgataa acaagctgat
     6241 ggtactgccg ccctgttgtg ccagtaccca gaacaacgtc ccgacaaaca tcaacaatac
     6301 aatttgccaa agagcacggc ggtgttcggg gaatttgatc atcatgcggg caatgatttg
     6361 tgctgcaatg aggcaaacga tcgccagcaa atatccggac cagtcgttct ccagcagcag
     6421 ggtaaaaaat actggggcta aacagagcat caccactaac cagctccata ctggtaaggc
     6481 aaatttgacg ctagtgagcg cttttttatc catactacgc gtggactgga aatgacgatg
     6541 accacttaag aaaatcaaca aaccgataaa catgccgcct cccgcaaggg caaagccaac
     6601 atgccatcca taccactgag cagccaggcc gcaggcgatg ggcgcggcga tagaaccgat
     6661 attgcccgca gcatatagca gcgaaaaacc gccatcacgt cgatgatcgt tctcgtcgta
     6721 gagctcgcca agcaaacagc tgatgtttga tttgaataaa ccgtagccac aaataatgat
     6781 tgccagcgcc agatacaggc taaaggttga atttgtatca atgcccagca ccacatggcc
     6841 aagggtcatt aacagcgcgc cggcaatcac tgcagtgcgg ttgccgagca ggcggtcggc
     6901 aagccagccg ccgagaatag gggtaacgta aaccagagaa gcatatgcgc tgaacaggct
     6961 gatggcatgg ttatcatcaa aaccaagctg atgggtgaga tagagaatga gtaaggcacg
     7021 catgccgtaa aaactgaagt actcccagat ttggatcgcc acgatatagt atatcgcgcg
     7081 cggctgtgag ggtgttttca tgtgttctcc ttatgagcaa aaaagggaag tggcaagcca
     7141 cttcccttgt acgagctaat tattttttgc tttcttcttt caatacctta acggtatagc
     7201 ggccatcagc ctgacggtat gcaccgtgaa tatcggtttc aaagcccgga tagtgagcgc
     7261 cgatttcaca cagcatctgc aggaactcca gaaccggacg gctttcttcg gtgatcattt
     7321 cacccggcat taccagagga actcccggcg gatacggaag gatcatattg gcgttaatac
     7381 gacctaccat ttcgtcgagg taaacttctt cggtcatacc gtgcagctct ttctggaacg
     7441 ccgcatacgg agtcataacc attgtcggca gaacttcaaa tgcgcgatac atcagatccg
     7501 gcagattgtg gtgaacaatc agtttgtgga tattctgagc cagttcctga atacgcatat
     7561 cttcatagaa ttcaggatct tcacgatata gagacggcag catgtttttc acacgcaggt
     7621 tcaggtcgaa cgcacgtttg aagtcagtca gagcacgcag caggctcaat gctttggtct
     7681 tatcaatacc gatgctgaac aggaacagca ggttatacgg gccggttttc tcaacaacga
     7741 tgccatgttc gtcgaggtat ttcgccacga tgctggccgg aataccaaag tcgctcatgg
     7801 tgccgtcttt ttccatcccc ggggtcagca gggtgacttt gatcgggtca agatacatgt
     7861 gctcattgtc gatgttcttg aagccgtgcc aggagctgtc agaacgcagc ggccagcatt
     7921 cagtggtatc gatatgatcc ggctgccata catcaaagaa ccagccatca gattccgttc
     7981 tcagacgttt gatctcttta cggaacttga tcgcacgtga tcttacccag caatagtgga
     8041 cacgcggcta agtgagtaaa ctctcagtca gaggtgactc acatgacaaa aacagtatca
     8101 accagtaaaa aaccccgtaa acagcattcg cctgaatttc gcagtgaagc cctgaagctt
     8161 gctgaacgca tcggtgttac tgccgcagcc cgtgaactca gcctgtatga atcacagctc
     8221 tacaactggc gcagtaaaca gcaaaatcag cagacgtctt ctgaacgtga actggagatg
     8281 tctaccgaga ttgcacgtct caaacgccag ctggcagaac gggatgaaga gctggctatc
     8341 ctccaaaagg ccgcgacata cttcgcgaag cgcctgaaat gaagtatgtc tttattgaaa
     8401 aacatcaggc tgagttcagc atcaaagcaa tgtgccgcgt gctccgggtg gcccgcagcg
     8461 gctggtatac gtggtgtcag cggcggacaa ggataagcac gcgtcagcag ttccgccaac
     8521 actgcgacag cgttgtcctc gcggctttta cccggtcaaa acagcgttac ggtgccccac
     8581 gcctgacgga tgaactgcgt gctcagggtt acccctttaa cgtaaaaacc gtggcggcaa
     8641 gcctgcgccg tcagggactg agggcaaagg cctcccggaa gttcagcccg gtcagctacc
     8701 gcgcacacgg cctgcctgtg tcagaaaatc tgttggagca ggatttttac gccagtggcc
     8761 cgaaccagaa gtgggcagga gacatcacgt acttacgtac agatgaaggc tggctgtatc
     8821 tggcagtggt cattgacctg tggtcacgtg ccgttattgg ctggtcaatg tcgccacgca
     8881 tgacggcgca actggcctgc gatgccctgc agatggcgct gtggcggcgt aagaggcccc
     8941 ggaacgttat cgttcacacg gaccgtggag gccagtactg ttcagcagat tatcaggcgc
     9001 aactgaagcg gcataatctg cgtggaagta tgagcgcaaa aggttgctgc tacgataatg
     9061 cctgcgtgga aagcttcttt cattcgctga aagtggaatg tatccatgga gaacacttta
     9121 tcagccggga aataatgcgg gcaacggtgt ttaattatat cgaatgtgat tacaatcggt
     9181 ggcggcggca cagttggtgt ggcggcctca gtccggaaca atttgaaaac aagaacctcg
     9241 cttaggcctg tgtccatatt acgtgggtag gatcactact catactataa gagggtccac
     9301 ttccccccgg atatatgatg atggatggag tatccggagc ccagcatgct ccatta
//
LOCUS       DAZFLD010000181         9309 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.182, whole genome shotgun sequence.
ACCESSION   DAZFLD010000181 DAZFLD010000000
VERSION     DAZFLD010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9309
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.182"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            390..1880
                     /locus_tag="ACHKBL_003839"
     CDS             390..1880
                     /locus_tag="ACHKBL_003839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001513421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfjI family protein"
                     /protein_id="HFO5820643.1"
                     /translation="MPYFQYPDEFPLSSLPPLIRDAVIEAQQITQAPLGLVAASALGA
                     VSLVCQNLIDVCRLNTLRGPVSLFLLTLAESGERKTAVDKLLMEPLYQQEMLLYSRHK
                     NELTTWKNKEELLKAQKKALLSKLNKELRKGADESETLRQLEALQKNRGEKPVRYKFI
                     FNDATTAAIKDQLCGQWRSVGIMSDEAGIIFDGYTLSELPFINKMWDGSVLSVDRKNE
                     PEQMIENARMTLSLMVQPGLFDRYMERKGSVARDSGFLARCLISKPATTQGKRFINGA
                     VTPGESLTAFHERLMELARGSIEKSSKDERYCLHFSPEAQKIFIDHYNVLEQDLSPSG
                     PLSQFRGHVSKKTENIARIAALLQYFSQGEGKISADIMTSAVVISSWYTDEYKKLFAL
                     PDESELQLQDARELLDWLIEECRGECPPRVRKNYILQCGPGRFRNRKKLNVLLNILAS
                     QLRLSVVQEGKTTYVLLSEINNITLPELNACYSQKLIRWHEQSFGL"
     gene            complement(2002..2574)
                     /locus_tag="ACHKBL_003840"
     CDS             complement(2002..2574)
                     /locus_tag="ACHKBL_003840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5820644.1"
                     /translation="MQKLENRCELLLIQHQKWMTSVTRLIVAHGMGSPHLHGYHRLTL
                     AHFFLPEKGTIISVAPQGLYQVVNPGTPPFIPAIQEGLMTSIQTHEIMLLTHFNLGGV
                     LLSELHRLGENRLANRLNSLLRRFDDRDLYHTLIWLCWYDLMCAHSMQPWTEELKHKS
                     HAELENWAVARKREKRELELMIDEYLLYAC"
     gene            complement(2660..2866)
                     /locus_tag="ACHKBL_003841"
     CDS             complement(2660..2866)
                     /locus_tag="ACHKBL_003841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO5820645.1"
                     /translation="MNTPVSLMDDQMVDMAFITQLTGLTDKWFYKLIKDGAFPAPIKL
                     GRSSRWLKSEVETWLQARIAQSRP"
     gene            3723..3947
                     /gene="hha"
                     /locus_tag="ACHKBL_003842"
     CDS             3723..3947
                     /gene="hha"
                     /locus_tag="ACHKBL_003842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HFO5820646.1"
                     /translation="MQHKVTRDEWKLKPEQFRRCSSRETLEKVITRTRYKLTPAELEA
                     FNSAVDHRLAELTMNKLYDRVPASVWKYVT"
     gene            complement(4279..4476)
                     /locus_tag="ACHKBL_003843"
     CDS             complement(4279..4476)
                     /locus_tag="ACHKBL_003843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001547002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5820647.1"
                     /translation="MVIRKKKCRDCGNAITHNTVCCPYCGAVDPFGYYRKTDRLLCLL
                     TLLLVLILVTVSGVSVFVLLQ"
     gene            4675..5244
                     /locus_tag="ACHKBL_003844"
     CDS             4675..5244
                     /locus_tag="ACHKBL_003844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
                     /protein_id="HFO5820648.1"
                     /translation="MTYKYNPFWQQRIRETVRHALDVHPRLTALRVDLRLPDVPAATD
                     AAVISRFINALKARIDAYQKRKHREGKRVHSSTLHYAWAREFGELKGKKHYHLLLLVN
                     RDTWCRAGDYRAPGSLAGMIKQAWCSALGVDAGRYDTLAHFPDKPAVWLERDDETGFQ
                     QVLERSGYLAKEHTKARGTGERNFGCSRS"
     gene            5505..5906
                     /locus_tag="ACHKBL_003845"
     CDS             5505..5906
                     /locus_tag="ACHKBL_003845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004020115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc ribbon protein"
                     /protein_id="HFO5820649.1"
                     /translation="MYAKSFLALDGNGRLTGARTAQTAPYDRYTCHLCGSALRYHPQY
                     DTERPWFEHTDDGLTKHGHECPYVRPERREVRLIKRLQQFVPDALPVVRKASWHCRQC
                     HHDYYGEQYCTNCQTGGFSIPRTTQEEICEF"
     gene            5894..6328
                     /locus_tag="ACHKBL_003846"
     CDS             5894..6328
                     /locus_tag="ACHKBL_003846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001556209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HFO5820650.1"
                     /translation="MRILNCYMANDSKGHFVTAKEAAKHNRQDVLCCVSCGCPLTLQR
                     GNDGQPPWFEHDQMTVAEKILLRCTWLDPAEKEARRLHLQGMTVPDYTVKVRKWFCVM
                     CDEDYEGEKCCPRCGTGVYSRAWGRQEVPSEDARADNPLQRL"
     gene            7103..7329
                     /locus_tag="ACHKBL_003847"
                     /pseudo
     CDS             7103..7329
                     /locus_tag="ACHKBL_003847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013364090.1"
                     /note="with IlvB catalyzes the formation of 2-acetolactate
                     from pyruvate, the small subunit is required for full
                     activity and valine sensitivity; E.coli produces 3
                     isoenzymes of acetolactate synthase which differ in
                     specificity to substrates, valine sensitivity and affinity
                     for cofactors; also known as acetolactate synthase small
                     subunit; frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
     gene            complement(7331..8113)
                     /locus_tag="ACHKBL_003848"
     CDS             complement(7331..8113)
                     /locus_tag="ACHKBL_003848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HFO5820651.1"
                     /translation="METKQKERIRRLIEILKKTDRIHLKDAARMLEVSVMTIRRDLHQ
                     EDEPLPLTLLGGYIVMVHKPAPSMPVIQDVPRNHRDDLPIAILAAGMVNENDLIFFDN
                     GQEIPLVISMIPDAITFTGICYSHRVFVALNEKPNVTAILCGGTYRARSDAFYDASNS
                     SPLDSLNPRKIFISASGVHDHFGVSWFNPEDLATKRKAMARGLRKILLARHALFDEVA
                     SASLAPLSAFDVLISERPLPADYVTHCRNASVKIITPDSEDE"
     gene            8416..>9309
                     /gene="rbsK"
                     /locus_tag="ACHKBL_003849"
     CDS             8416..>9309
                     /gene="rbsK"
                     /locus_tag="ACHKBL_003849"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006014 - D-ribose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HFO5820652.1"
                     /translation="MDIAVIGSNMVDLITYTNQMPKEGETLEAPAFKIGCGGKGANQA
                     VAAAKLNSKVLMLTKVGDDIFADNTIRNLESWGINTTYVEKVPCTSSGVAPIFVNANS
                     SNSILIIKGANKFLSPEDIDRAAEDLKKCKLIVLQLEVQLETVYHAIEFGKKNGIEVL
                     LNPAPALRELDMSYACKCDFFIPNETELEILTGMSVDTYDHIRLAARSLVDKGLNNII
                     VTMSEKGALWMTRDQEVHVPAFKVNAVDTSGAGDAFIGCFSHYYVQSGDVEAALKKAA
                     LFAAFSVTGKGTQSSYPSIEQF"
BASE COUNT         2431 a         2085 c         2403 g         2390 t
ORIGIN      
        1 ccatagaata ttgtactgag tactcagcat tcagcgcagg aactgtcatc ttctttattt
       61 tattcttctt caggagggca gttttctgaa agcgttactc cggggaaact gtttatgttt
      121 attcccggca ggaaacccgt acctgcaata ccctggatga tgttttttgc tggtacgttg
      181 ttgtgtgaaa attaatcaac atgtgtgctc cggagagcgc ccgtagacgc acagattcat
      241 tctgacattc tttagcgaca aaataccggg tggttattat ttaacctgaa aatcgacaga
      301 aatttttatc ctgtattgat tctgctgcta tttttgctat tagtagcaat agttgcggta
      361 ctttatgttc tgtgtgttgt gaggtcatta tgccatactt tcagtatcct gatgagtttc
      421 ccctgagctc gctgcctcca ctcattcgtg atgccgttat tgaagctcag caaatcacgc
      481 aggctcccct ggggcttgtg gcagcatctg ccctgggcgc tgtttcactt gtctgccaga
      541 atctgattga tgtctgcaga ctgaatacac tgcgggggcc ggtttctctt ttcttactga
      601 cgcttgctga gtccggagaa cgaaaaaccg cagtggacaa attattaatg gagcccctgt
      661 accagcagga gatgctgtta tattccagac acaaaaatga actgacgaca tggaaaaata
      721 aggaagagct tttaaaggcg cagaaaaaag cattgttgtc aaaactgaat aaagaactcc
      781 gcaaaggcgc ggatgagtca gaaacgctca gacaactgga agcgcttcag aaaaacagag
      841 gggaaaaacc tgtaagatat aaattcattt ttaatgatgc cacgacagca gcaattaaag
      901 atcagctttg tggtcagtgg cgttcagtgg gtatcatgtc tgatgaagcc ggtataatat
      961 ttgatgggta taccctgtct gagctgcctt ttattaacaa aatgtgggat ggctccgtat
     1021 tgtcggtgga cagaaagaat gaacccgaac agatgattga aaatgccaga atgacattat
     1081 ccctcatggt acaacccggt ctttttgacc gatatatgga gcgaaaaggt tctgttgcca
     1141 gggacagtgg ttttcttgcc cgttgtctaa tcagtaaacc tgccaccacg caggggaaac
     1201 gctttattaa cggagcagtg accccgggag aatcattaac ggcatttcat gaacgattaa
     1261 tggaactggc cagaggcagt attgaaaaat ccagtaagga tgaacgttat tgtctgcatt
     1321 tttctccaga agcgcaaaaa atatttattg atcactataa tgttctggag caggatttgt
     1381 ctccttccgg acccttaagc cagtttcggg ggcatgtttc aaaaaaaaca gaaaacattg
     1441 cgagaattgc tgcgttattg cagtatttca gtcagggtga agggaaaatc tctgccgata
     1501 tcatgacgtc tgctgtcgta ataagttcgt ggtacacaga tgaatataaa aaattatttg
     1561 cgttacctga tgaatctgaa ctgcagctgc aggacgcccg ggagctgctc gactggctca
     1621 ttgaggagtg ccgcggagaa tgcccgcccc gtgtcaggaa aaattatatt ctccagtgtg
     1681 gcccgggccg gttcaggaac aggaaaaagc tgaacgtcct gctgaacatt cttgcatctc
     1741 aactcaggct ctctgtcgtg caggaaggaa aaaccaccta tgtcctgctt tccgaaataa
     1801 acaatatcac gctgcctgaa ctgaatgcgt gctacagcca gaaactgata agatggcatg
     1861 agcaatcctt tggcttataa tgttccctgt tgctgagacg gattattcag tggtggtatc
     1921 gcagtatgcc cgtcaccccc cgcttctgca tgagggtgac ggaggaaaag taagtgtgcg
     1981 gaggcccggg agcgttatga atcaacaggc gtataacagg tactcgtcaa tcatcagttc
     2041 cagctcgcgc ttttcccgtt tgcgcgccac agcccagttt tcaagctctg catgtgattt
     2101 atgctttaac tcttcagtcc agggctgcat ggagtgggca cacatcagat cataccagca
     2161 cagccatatc agcgtgtggt agagatcacg atcgtcgaaa cgccggagca ggctgttcag
     2221 cctgtttgcc agccggtttt cacccagacg atgtagctca ctgagtaaaa cgccgcccag
     2281 attaaaatgt gtaagcagca tgatttcatg ggtctgaatg ctggtcatta acccttcctg
     2341 aatggcagga atgaacggtg gtgttcccgg gttgacgacc tgatagagtc cctgtggtgc
     2401 cactgagatg atagtgcctt tttccggcag aaaaaagtgg gccagggtca ggcgatgata
     2461 accatgaaga tgcggagacc ccattccatg agcgacgata agcctcgtca cgcttgtcat
     2521 ccatttctga tgctggatga gcagcagctc acaccggttt tcaagttttt gcataatatt
     2581 ctctccttta gcgcgcagca aaatgcctcc gggggccgga tcatgccttt gctgcggttg
     2641 aacggataac ggtcaggaat tacggacggg actgtgcaat acgcgcctgc agccaggttt
     2701 ccacttcact tttcagccat cgggagctac ggcccagttt gatgggggca ggaaaggcac
     2761 catccttgat gagcttgtaa aaccatttat cggtcaggcc ggtcagttga gtgataaatg
     2821 ccatgtcgac catctggtca tccatcagtg aaactggggt attcatgaga atgttccttc
     2881 ggatgttgtt aatctcaacc ggcgttggtg atgaaaacaa cgcaccacct tcaccggcat
     2941 aacaaccggg gagaaacgta aaaaataatc gggaatgtga ccgtcaggag ataaacgggg
     3001 ggattgcggt ggctgagccg taccggtatg tcgggtgatt gttgcgaaac gatgcttccg
     3061 cacgcgcacg ggaggacttt cgtgagacaa ggggaaaact ggcgttctga taattgcaga
     3121 gttgtttttg aaggcggttg tggtgcatca gcctgtgacc tgtgggcagg cgacaggtat
     3181 cactggtggg ataagactgt ctgattggta ttataaatat ataagagagc tcaaacacat
     3241 gagttcatat atgtgaaata aatctgtgag tgtgatcagc agaccagtct acccagtagc
     3301 aacagattgc gttgtactca cagggttatc cctgataact ggcgtataac ctgcgtacat
     3361 aaaacgtacc tgcaatgcac ctgaacagag tgcattaagc atccgcctga gcggtaaacg
     3421 tatccgatct ggtgagtgac agtctgagag cattctcagt aatcgccccc ttgtatcctg
     3481 gaaattggtt aagagcgatt agatttggca aggtgtatgc atccagcagc ttaacacctc
     3541 agctaactgg ctggttacct tcaggttgtc gcaatggcat cggacatccg gtgtcggata
     3601 actaaacgta gcgttaaggc gatattgctg agagaccggt taagtggtgg cacgggagta
     3661 atgagtttgg ggagggtacc agaatgccag tcaggtgcgg tatgctggca gtatcacgtt
     3721 gattgcagca taaagtaacg agagacgaat ggaagttaaa accagagcag ttccgtcgtt
     3781 gttcatcccg ggagacgctg gaaaaagtga ttacccgcac acgttataaa cttacccctg
     3841 cggagctgga agcctttaac tctgcggtcg atcaccggct ggcagaactg acaatgaaca
     3901 aactttacga tcgcgtgccg gcttccgtct ggaaatatgt cacctgaatc ttgaatccgc
     3961 gctacgatga ctctggtgtt atctgtgcac cggaagatcg ggaacattaa gcagtataac
     4021 ggcaacaaag aattcgaaat caaaagggaa tggtgtacag aggggcaggc gttcaggcat
     4081 taccggcctg tatcttctga aggggactcg tatttgtcgg ggctgagtat tccgctcgat
     4141 tcgatcacgg attggcagaa acctgactgt gattgattca ggtaccattt tgcagggatc
     4201 cggtgcgtta ctgtctggca caacaaacgc agcgggtcgc tctgccccgc cgattcctga
     4261 taattcctcc cctgtaacct actgcaacag tacaaataca ctgacgcctg aaacggtaac
     4321 cagaatcagc accagcagta acgtcagtaa acagagcaga cgatcggtct tccggtaata
     4381 accgaagggg tcgacagcgc cacaatacgg gcaacacact gtattatgag taattgcgtt
     4441 tccacagtcc ctgcacttct ttttgcgtat cacgatcgct ccctctccac gccttatccg
     4501 taacctgttt tttacattaa aaaaacaacg ccgggaattc aatcgtcagt ccggagacgg
     4561 ccgttcgggt tatcacagag tgcctgagac agtgtcctgc cggaggtcac ctcaggggct
     4621 ctgcgcattt tttacgacgt ggctattccg tatgaaccat acggagattt aaccatgacc
     4681 tacaaataca accccttctg gcagcaacgt attcgtgaga cggtgcggca cgcactggat
     4741 gttcatcccc gcctgacggc acttcgggtt gatctgcgtc tcccggatgt gccggcagca
     4801 acggacgcgg ctgtgatatc ccgcttcatc aatgccctga aagcccgaat cgacgcttat
     4861 cagaaacgta agcatcggga aggtaaacgc gtgcattcct cgaccctgca ttacgcctgg
     4921 gcccgggagt tcggggagct taaaggcaaa aaacactatc acctgctgct gctggtcaac
     4981 cgggatacct ggtgtcgtgc cggcgattac cgcgctccgg ggtcactggc cgggatgatt
     5041 aaacaggcct ggtgcagtgc cctgggagtg gatgccggac gttatgacac gctggcacat
     5101 tttcctgaca agccagcggt gtggctggaa cgcgatgatg aaaccggctt tcagcaggtg
     5161 ctggaacgtt ctggctatct ggcgaaggag cataccaaag ctcgcggcac cggtgagcgc
     5221 aattttggct gtagccggag ttgaacacga caggcgcacc aatacccggc aaaccaaaag
     5281 tatgggttgt tgtctggcgg caacgatgta tccactcgcc cactgactgt tttttacgtc
     5341 agtgggccat tgtgtgacaa ccacagtgct gtaacagcaa ccagcctgct ttacccaaac
     5401 tccagtccag attttaactg aaccgccatt cacaccctga ccacgctgcc cgtaccggga
     5461 cggctgttgc ctgcgtgtcg tctggcggct gaggaacata tactatgtac gcaaaatcct
     5521 ttctcgctct tgatggcaac ggacgtctga cgggggcccg taccgcacag actgcgcctt
     5581 acgaccgcta cacctgccac ctttgcggca gtgcactcag gtaccacccg caatacgaca
     5641 ctgaacgtcc ctggtttgaa cacactgacg acgggctgac taagcacggt catgagtgcc
     5701 cttatgtcag accggaacgc agggaggttc gcttgattaa gcgtctgcag caattcgtac
     5761 cggatgcctt acccgtggtg cgtaaagcca gctggcactg cagacaatgt caccacgatt
     5821 attatgggga gcagtactgc acaaactgcc agaccggagg attcagtatt ccccggacaa
     5881 ctcaggagga aatatgcgaa ttctgaactg ttatatggcg aatgacagca aaggccattt
     5941 tgttacggcg aaagaagctg cgaagcacaa ccgacaggac gttttgtgct gtgtgtcctg
     6001 tggatgcccg ttaacacttc agcggggcaa tgacggacaa ccaccgtggt ttgaacatga
     6061 ccagatgact gtcgctgaaa aaatcctgct gcgatgtacc tggcttgacc cggcagagaa
     6121 agaggcccgt cgtttgcatc tgcagggcat gacggttccg gattatacgg tgaaggtgag
     6181 aaagtggttt tgtgtgatgt gtgacgaaga ttatgagggg gaaaagtgct gcccacgctg
     6241 cggtactggg gtatacagca gggcgtgggg gcggcaggag gtgccgtcgg aagatgccag
     6301 ggctgataat ccgttacaga ggctgtaatg gttgcctccg gagcagtttg cggtaatgcc
     6361 gttcccggga atggattgcc agtgcgggga ctgtggagct taattccggt gttggtggcc
     6421 tttccgttga ttttgagcca acagccctcc tgtgactgac aggctgcctc gtcattccat
     6481 tcgtatctcc agtaacaggg ggttgtattg tattttaatg tgtcggttca ggcgtgcgtt
     6541 tcccggcgcg tctgcaccgg cttaaccaaa ttcaacaggg ataaaatagt gtaaataacc
     6601 gaagtattac ttgcagaatc gggggaagca tccggggttt tattcaacag gcatgggagg
     6661 ctccggctct gacagacatt gtactgtgga atgttgttac aggttggggc ggcaaccgtg
     6721 ccaggagacc atgagccgtt cacggcatta aaggccacac ttcggtcgtt tacattccct
     6781 gtggtggcag gcatcttccg ttaaggcgtc agagcgttac caacgttatg ttgtcatggc
     6841 tcacctttca tagtcgatgc ttatctggtg actgaaacta gccgcagata aagagtgtga
     6901 agttgatacc atggagtgga gtgacgtgca ggcaggagat tcgctctatg cagggttgtg
     6961 agatgaattc aaaccggctg tcagcgccag taatatttga agattcgtcc ggttgttatc
     7021 cagtgtgcat taaaaaccca gacattatgg acaatacaac ggcttccata acagggagga
     7081 gcctgtttat tatccgggcg taatgactaa cctttgtggg ctttttcccg tcgtgctttt
     7141 aacgttgaag gcattttctg cctgccgatt caggacagcg acaaaagcca tatcagacaa
     7201 ttggtcaatg atgaccagcg tctggagtag atgataagcc aaattgataa actggaatat
     7261 gtgatgaaag tgcaacgtaa tcagtccaat ccgacgatgt ttaacaagag tacggtgttt
     7321 tttcagtaag tcattcgtct tctgaatcag gtgtaatgat ctttacagaa gcattccggc
     7381 agtgcgtaac ataatctgcc ggtaacggac gctcgctaat cagaacatca aatgcagaga
     7441 gcggtgcgag gctggcagag gctacttcat cgaacaaggc gtggcgggcg agcaaaattt
     7501 tccttagtcc acgggccatc gctttacgct tagtggcaag atcttcggga ttaaaccagc
     7561 tgacgccaaa gtgatcatgt acaccgctgg cggaaataaa tatttttcgc ggattgagag
     7621 agtctaatgg cgaagagtta ctggcatcgt aaaaagcatc acttctggca cgatacgtac
     7681 caccacaaag tattgctgtc acattaggtt tttcattcaa cgcaacaaag acacgatgtg
     7741 agtaacagat gccagtgaag gtgattgcat ccgggatcat gcttataacg agcggtatct
     7801 cctggccatt atcaaagaag atcagatcat tttcattaac cattccggcg gccagaattg
     7861 caataggtaa gtcatcacga tgatttctcg gaacgtcctg gattactggc atggatggtg
     7921 cgggtttatg caccattaca atatagccac ccagtagggt cagtggcaga ggttcatctt
     7981 cctgatggag atcgcgacga atagtcatta cagaaacttc cagcattcgt gccgcgtctt
     8041 tcaaatggat tctgtcggtt ttcttaagta tttcaatcaa acgtcggata cgctcttttt
     8101 gcttcgtttc catcaactct ctccgtaaaa tgttataaaa ataacattat ggcattagta
     8161 aacattgttt tagttaccat actatacgac cgaccctttg aaatgaaaag ggtatagact
     8221 gctgaatttt cggtgttaat cctgttaggt tgcttatgat tagatagtgt tggcctgagt
     8281 gataagactg gaagtctatt aaataaacat cgggttgatg tgctaatcgc gatctcagtc
     8341 acaaatgatg caaaagtaac atgctaatgt tatttaaata ccattgttat gcgaaagttt
     8401 ataagaggta gtgaaatgga cattgcagtt attggttcca atatggtgga tctcattact
     8461 tacactaatc agatgcctaa agaaggagaa actctggaag cacctgcctt caaaatcgga
     8521 tgtggtggga aaggtgctaa tcaagcggtg gcggcggcta aattgaattc aaaagtcctg
     8581 atgctgacta aagtaggtga tgatattttt gcggataata ctattcgtaa tctcgaatca
     8641 tggggaatta ataccactta tgttgagaaa gtcccctgta ccagcagtgg tgttgcaccg
     8701 atctttgtta atgccaactc gagcaatagt atattaataa ttaagggagc gaataaattt
     8761 ctttccccgg aagatatcga tcgagctgca gaagatctga aaaaatgcaa attgatcgtt
     8821 ttacaactgg aagttcaact ggaaacagtt taccacgcga ttgagtttgg taagaaaaat
     8881 ggtattgaag tgttattaaa tccggctccg gcattgcgtg agttagatat gtcttatgcc
     8941 tgcaaatgtg atttctttat cccgaatgaa acagaactgg aaatattaac cggtatgtcg
     9001 gttgatactt atgatcatat tcgtttggcc gcacgttcgt tagttgataa agggctgaac
     9061 aatattattg ttaccatgag cgaaaaaggc gcattatgga tgacccgcga tcaggaagtc
     9121 catgttcctg cattcaaagt aaatgctgtt gatactagtg gcgctggtga cgcatttatt
     9181 ggttgctttt cccattatta tgttcaaagc ggtgatgttg aagccgcttt gaaaaaagcc
     9241 gctctctttg ccgctttcag cgtcacaggg aaaggtacgc aatcatctta tccgagtatt
     9301 gaacaattt
//
LOCUS       DAZFLD010000182         9297 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.183, whole genome shotgun sequence.
ACCESSION   DAZFLD010000182 DAZFLD010000000
VERSION     DAZFLD010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9297
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.183"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(167..2434)
                     /gene="adiA"
                     /locus_tag="ACHKBL_003850"
     CDS             complement(167..2434)
                     /gene="adiA"
                     /locus_tag="ACHKBL_003850"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418541.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HFO5820653.1"
                     /translation="MKVLIVESEFLHQDTWVGNAVERLADALSQQNVTVIKSTSFDDG
                     FAILSSNEAIDCLMFSYQMEHPDEHQNVRQLIGKLHERQQNVPVFLLGDREKALAAMD
                     RDLLELVDEFAWILEDTADFIAGRAVAAMTRYRQQLLPPLFSALMKYSDIHEYSWAAP
                     GHQGGVGFTKTPAGRFYHDYYGENLFRTDMGIERTSLGSLLDHTGAFGESEKYAARVF
                     GADRSWSVVVGTSGSNRTIMQACMTDNDVVVVDRNCHKSIEQGLMLTGAKPVYMVPSR
                     NRYGIIGPIYPQEMQPETLQKKISESPLTKDKAGQKPSYCVVTNCTYDGVCYNAKEAQ
                     DLLEKTSDRLHFDEAWYGYARFNPIYADHYAMRGEPGDHNGPTVFATHSTHKLLNALS
                     QASYIHVREGRGAINFSRFNQAYMMHATTSPLYAICASNDVAVSMMDGNSGLSLTQEV
                     IDEAVDFRQAMARLYKEFTADGSWFFKPWNKEVVTDPQTGKTYDFADAPTKLLTTVQD
                     CWVMHPGESWHGFKDIPDNWSMLDPIKVSILAPGMGEDGELEETGVPAALVTAWLGRH
                     GIVPTRTTDFQIMFLFSMGVTRGKWGTLVNTLCSFKRHYDANTPLAQVMPELVEQYPD
                     TYANMGIHDLGDTMFAWLKENNPGARLNEAYSGLPVAEITPREAYNAIVDNNVELVSI
                     ENLPGRIAANSVIPYPPGIPMLLSGENFGDKNSPQVSYLRSLQSWDHHFPGFEHETEG
                     TEIIDGIYHVMCVKA"
     gene            complement(2633..3541)
                     /gene="melR"
                     /locus_tag="ACHKBL_003851"
     CDS             complement(2633..3541)
                     /gene="melR"
                     /locus_tag="ACHKBL_003851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MelR"
                     /protein_id="HFO5820654.1"
                     /translation="MNTDTFMCSSDEKQTRSPLSLYSEYQRMEIEFRAPHIMPTSHWH
                     GQVEVNVPFDGDVEYLINNEKVNINQGHITLFWACTPHQLTDTGTCQSMAIFNLPMHL
                     FLSWPLDKDLINHVTHGMVIKSLATQQLSPFEVRRWQQELNSPNEQIRQLAIDEIGLM
                     LKRFSLSGWEPILVNKTSRTHKNSVSRHAQFYVSQMLGFIAENYDQALTINDVAEHVK
                     LNANYAMGIFQRVMQLTMKQYITAMRINHVRALLSDTDKSILDIALTAGFRSSSRFYS
                     TFGKYVGMSPQQYRKLSQQRRQTFPG"
     gene            3824..5179
                     /gene="melA"
                     /locus_tag="ACHKBL_003852"
     CDS             3824..5179
                     /gene="melA"
                     /locus_tag="ACHKBL_003852"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFO5820655.1"
                     /translation="MMSAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPT
                     RLEESHIVVRKLMDSAGASGKITCHTQQKEALQDADFVVVAFQIGGYEPCTVTDFEVC
                     KRHGLEQTIADTLGPGGIMRALRTIPHLWQICEDMTEVCPDATMLNYVNPMAMNTWAM
                     YARYPHIKQVGLCHSVQGTAEELARDLNIDPATLRYRCAGINHMAFYLELERKTADGS
                     YVNLYPELLAAYEAGQAPKPNIHGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWF
                     IKPGREDLIERYKVPLDEYPKRCVEQLANWHKELEEYKNASRIDIKPSREYASTIMNA
                     IWTGEPSVIYGNVRNDGLIDNLPQGCCVEVACLVDANGIQPTKVGTLPSHLAALMQTN
                     INVQTLLTEAILTENRDRVYHAAMMDPHTAAVLGIDEIYALVDDLIAAHGDWLPGWLH
                     R"
     gene            complement(5176..5826)
                     /gene="yjdF"
                     /locus_tag="ACHKBL_003853"
     CDS             complement(5176..5826)
                     /gene="yjdF"
                     /locus_tag="ACHKBL_003853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HFO5820656.1"
                     /translation="MTRTLKPLILNTGALALTLILIYTGISAHDKLTWLMEVTPVIIV
                     VPLLLATAKRYPLTPLLYTLIFFHAIILMVGGQYTYAKVPVGFEVQEWLGLSRNPYDK
                     LGHFFQGLVPALVAREILVRGMYVRGRKMVAFLVCCVALAISAMYELIEWWAALAMGQ
                     GADDFLGTQGDQWDTQSDMFCALLGALTTVIFLARFHCRQLRRFGLITRAPEAITP"
     gene            complement(5949..7595)
                     /gene="fumB"
                     /locus_tag="ACHKBL_003854"
     CDS             complement(5949..7595)
                     /gene="fumB"
                     /locus_tag="ACHKBL_003854"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HFO5820657.1"
                     /translation="MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEP
                     EALTLLAQQAFHDASFMLRPAHQKQVAAILHDPEASENDKYVALQFLRNSEIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEEALSKGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAHYYDELPTEGNEHGQAFRDVQLEQEL
                     LEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINREGIW
                     IEKLEHNPGQYIPQELRQAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVG
                     RDIAHAKLKELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDL
                     LQSHGGSMIMLAKGNRSQQVTDACHKHGGFYLGSIGGPAAVLAQQSIKHLECVAYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIVNKQCANCTK"
     gene            complement(7673..9013)
                     /gene="dcuB"
                     /locus_tag="ACHKBL_003855"
     CDS             complement(7673..9013)
                     /gene="dcuB"
                     /locus_tag="ACHKBL_003855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuB"
                     /protein_id="HFO5820658.1"
                     /translation="MLFTIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQP
                     GKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTI
                     LCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
                     NVTFDGRHLEFLDLLAITIPSTLIGILAIGIFSWFRGKDLDKDEEFQKFISVPENREY
                     VYGDTATLLDKKLPKSNWLAMWIFLGAIAVVALLGADSDLRPSFGGKPLSMVLVIQMF
                     MLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAETMFGAHMSEIQGVLG
                     EMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYIL
                     PTYPSDLAAIQFDRSGTTHIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL"
BASE COUNT         2202 a         2321 c         2500 g         2274 t
ORIGIN      
        1 aaaaaaagga taaataacag gcaaaaaaaa gcgcggggtg ggaccgcgca aaaagcatga
       61 cattttttgt ttgcagcaat ttttatgaaa acgttgccat acttaaaagg cctcaagcca
      121 ggcggcgtaa tgttatttaa caattacgcc ttcagcggaa tagtggttac gctttcacac
      181 acataacgtg gtaaataccg tcaataattt cagtcccttc agtttcgtgt tcaaatccag
      241 ggaaatgatg gtcccaggat tgcagcgagc gtaaataact tacttgcgga ctgtttttat
      301 cgccgaagtt ttcaccagac agcagcatcg ggattcctgg cggatacggg ataactgagt
      361 ttgccgcgat gcgtcctggt aggttttcaa tggataccag ttcgacattg ttgtcgacaa
      421 tcgcgttgta cgcttctcgc ggggtgattt ccgccaccgg caggccggaa taggcttcgt
      481 tcaaccgtgc gccagggttg ttttctttca gccaggcaaa catggtgtca cccagatcgt
      541 gaatccccat gttcgcgtaa gtgtcaggat attgttcaac aagttccggc atcacctgcg
      601 ccagcggggt gttggcgtca tagtggcgtt tgaaggagca aagggtgtta accagagtcc
      661 cccatttccc acgggtcacg cccatagaga acaggaacat aatctggaag tcagtggtac
      721 gggtaggcac aatgccgtgg cgaccaagcc aggctgtgac cagcgccgcc ggaacaccgg
      781 tttcttccag ttcaccatct tcacccattc ccggagcaag gatgctgact ttaatcgggt
      841 cgagcatact ccagttatcc ggaatatctt tgaagccgtg ccagctttcg cccggatgca
      901 tgacccagca gtcctgaacg gtggtcagca gtttggttgg tgcgtcagca aagtcatagg
      961 ttttgccggt ttgtgggtcg gtgacgactt ctttgttcca cggtttgaag aaccagctac
     1021 cgtcagcggt gaactcttta tacagccgcg ccatcgcctg acggaaatca accgcttcgt
     1081 cgatcacttc ctgcgtcagt gacaggccgc tgttgccgtc catcatcgac accgccacgt
     1141 cgttggatgc gcagatggca tacagcgggg aggtggtggc atgcatcatg taggcctggt
     1201 tgaagcggga gaagttaatc gccccacgac cttcacgtac atgaatataa gaggcctgtg
     1261 acagcgcatt cagcagtttg tgggtggagt gggtggcgaa aacggtaggg ccgttgtgat
     1321 cgccaggttc gccgcgcatg gcatagtgat cggcatagat tgggttgaaa cgtgcatagc
     1381 cgtaccaggc ttcgtcaaag tgcagacgat cggaggtttt ttccagcaga tcctgcgctt
     1441 ctttagcgtt ataacacacg ccgtcatagg tacagttggt caccacgcag taagacggtt
     1501 tttgcccggc tttgtctttg gtcagcgggc tttcactgat tttcttctgc aaggtttcag
     1561 gttgcatttc ctgcggatag attggcccga taatgccgta gcggttgcgg cttggcacca
     1621 tgtagaccgg tttcgcgcct gtcagcatca agccttgttc gatggattta tggcagttac
     1681 ggtcaacgac cacgacatcg ttatcggtca tgcaagcctg catgatggtg cggttagagc
     1741 cggaagtacc gacgactacc gaccaggagc gatcggcacc aaatacgcgt gcagcatatt
     1801 tttcgctttc gccaaatgcg ccagtatggt caagcaaaga accgagggaa gttcgttcga
     1861 tgcccatgtc ggtgcggaac agattttcac catagtagtc atggtagaaa cgtccggcgg
     1921 gtgttttggt aaaaccaacg ccgccctggt ggcctggcgc tgcccaggaa tattcatgga
     1981 tgtcgctata tttcatcagc gcgctgaaca gtggcggcaa cagttgctgg cggtagcggg
     2041 tcatcgcggc aacggcgcgt ccggcgataa agtcggcggt atcttccaga atccaggcga
     2101 attcatcgac aagctccagc agatcccggt ccattgcggc gagggctttt tcccgatcgc
     2161 ccaacaggaa gaccggcacg ttttgttgac gctcatgaag cttaccgatc aattgtctga
     2221 cgttttgatg ttcgtccgga tgttccattt gatagctgaa catcaggcag tcaatggctt
     2281 cgtttgaaga gagaatggca aaaccatcat caaaggaggt ggatttaatc acggtaacat
     2341 tttgctggct taaagcatct gccagacgct caacggcgtt accgacccag gtgtcttgat
     2401 ggagaaactc gctttcaaca attaatactt tcatcattgc ttacccggtt atgaaggaat
     2461 cttcgttgcg ggcaagtatc ttccggcctt ttcgggctgt aaagcgcgtg aaaaacaaac
     2521 atttttgaaa tatttgtttt tataaacaat atgttatgtg attattttaa attattttca
     2581 ctgcttatta tcgacggcta aactattttt ttggctgata ctgatatcgt ctttagccgg
     2641 gaaacgtctg gcggcgctgt tggctaagtt tgcggtattg ttgcggtgac atgccgacat
     2701 atttgccgaa cgtgctgtaa aaacggctac tcgaacgaaa gcctgccgtc agggcaatat
     2761 cgagaatact tttatcggta tcgctcagta acgcgcgaac gtggttgatg cgcatcgcgg
     2821 taatgtactg tttcatcgtc aattgcatga cccgctggaa tatccccatt gcatagttgg
     2881 cgttaagttt gacgtgctca gccacatcgt tgatggtcag tgcctgatca tagttttcgg
     2941 caataaagcc cagcatctgg ctgacataaa attgcgcatg gcgcgagacg ctgtttttgt
     3001 gtgtgcgcga ggttttattg accagaatcg gttcccagcc agagaggcta aatcgcttga
     3061 gcatcaggcc aatttcatca atggcgagct ggcgaatttg ctcgttcgga ctgttcaatt
     3121 cctgctgcca gcggcgcact tcaaacgggc taagttgctg tgtcgccagt gatttgatca
     3181 ccatgccgtg agtaacgtgg ttaatcaggt ctttatccag cggccaggag agaaacagat
     3241 gcatcggcag attaaaaatc gccatgctct gacaggttcc ggtatctgtt agttggtgcg
     3301 gtgtacaggc ccagaacagc gtgatatgac cctgattgat attcactttt tcattgttga
     3361 tcaggtattc cacatcgcca tcgaaaggca cattcacttc gacctgacca tgccagtggc
     3421 tggtgggcat gatatgcggt gcgcgaaact caatctccat ccgctggtat tccgaatata
     3481 gcgacagcgg gctgcgggtc tgtttttcgt cgctgctgca cataaacgta tctgtattca
     3541 tggatggctc tctttcctgg aatatcagaa ttatggcagg agtgagggag gatgactgcg
     3601 agtgggagca cggttttcac cctcttccca cagcggcgag gggagcgtcc gagcatcatg
     3661 aggccgaaaa ctctgctttt caggtaactt attcccataa actcagattt actgctgctt
     3721 cacgcaggat ctgagtttat gggaatgctc aacctggaag acggaggttt tctgcagatt
     3781 cgcctgccat gatgaagtta ttcaagcaag ccaggagatc tgcatgatgt ctgcacccaa
     3841 aattacattt atcggcgctg gttcgacgat tttcgttaaa aatattcttg gtgatgtgtt
     3901 ccatcgcgag gcgctgaaaa cggcgcatat tgccctgatg gacattgatc ccacccgcct
     3961 ggaagagtcg catattgtgg tgcgcaagct gatggattca gcaggggcca gcggcaaaat
     4021 cacctgccac acccaacaga aagaagcctt acaggatgcc gattttgtcg tggtggcatt
     4081 tcagattggc ggttatgaac cttgcacggt gactgatttc gaggtctgta agcggcatgg
     4141 tctggaacaa accattgccg atacgttggg gccgggcggt attatgcgcg cgctacgtac
     4201 cattccgcat ctgtggcaaa tttgcgagga catgacggaa gtctgccccg atgccaccat
     4261 gctcaactac gttaacccaa tggcgatgaa tacctgggcg atgtatgccc gctatccgca
     4321 tatcaaacag gtagggctgt gccattcggt gcagggaacg gcggaagagc tggcgcgtga
     4381 cctcaatatc gacccggcta cgctgcgtta ccgttgcgca ggtatcaacc atatggcgtt
     4441 ttacctggaa ctggagcgca aaaccgccga cggcagttac gtgaatctct acccggaact
     4501 gctggcggct tatgaagcag ggcaagcacc gaagccgaat attcatggca atactcgctg
     4561 ccagaatatt gtgcgctacg aaatgttcaa aaagctgggc tacttcgtca cggaatcgtc
     4621 agaacatttt gctgagtaca caccgtggtt tattaagcca ggtcgtgagg atttgattga
     4681 gcgttataaa gtaccgctgg atgagtaccc gaaacgctgc gtcgagcagc tggcgaactg
     4741 gcataaagag ctggaggagt ataaaaacgc ctcccggatt gatattaaac cgtcacggga
     4801 atatgccagc acaatcatga acgctatctg gactggcgag ccgagtgtga tttacggcaa
     4861 cgtccgtaac gatggattga ttgataacct gccacaagga tgttgcgtgg aagtagcctg
     4921 tctggttgat gctaatggca ttcagccgac caaagtcggt acgttacctt cgcatctggc
     4981 cgccctgatg caaaccaaca tcaacgtaca gacgctgctg accgaagcca ttcttacgga
     5041 aaatcgcgac cgtgtttacc acgctgcgat gatggacccg catactgccg ccgtgctggg
     5101 cattgatgaa atatatgctc ttgttgacga cctgattgcc gcccacggcg actggctgcc
     5161 aggctggttg caccgttaag gggtaatcgc ttccggcgct ctggtgatta agccaaagcg
     5221 ccgtagctgg cggcaatgaa agcgagcgag gaatatcacc gtcgttaatg cgccaagcag
     5281 cgcgcagaac atatcggatt gcgtgtccca ctggtcgccc tgggtgccga gaaaatcatc
     5341 cgctccctga cccattgcca gcgccgccca ccactcgatt aattcataca tcgcgcttat
     5401 cgccagcgcc acgcagcaga ccagaaacgc caccatctta cgtccgcgaa cgtacatccc
     5461 gcgtacaagg atctctcgtg ccaccagtgc aggcaccagc ccctggaaaa aatgccccag
     5521 tttgtcatac ggattacggc tcaaccccag ccattcctgc acctcaaaac caacggggac
     5581 tttcgcgtag gtgtattgcc cgccgaccat caggatgatg gcgtgaaaga aaatgagcgt
     5641 ataaagcagc ggagttaacg gataacgttt agcggtggca agcagtagcg gcacgacaat
     5701 aatcaccggt gtcacttcca tcagccaggt gagtttgtca tgggccgaaa tgccggtata
     5761 aatcaggatt aacgttagcg ccagtgcgcc ggtgttaaga attaacggct tgagtgtgcg
     5821 ggtcatggtg agttcgcaag tcagggaaaa tactgactat tgaccagtaa gcgtgaaatt
     5881 acaatcgcaa aagaggtggc ccagggggat cacctggcag catgctgcca ggcgctgggc
     5941 cgaagaggtt acttagtgca gttcgcgcac tgtttgttga cgatttgctg gaagaagtcg
     6001 ttgcctttgt catcgaccag gataaacgcc gggaaatctt ctacttcgat tttccagata
     6061 gcttccatac ccagctccgg ataagcgacg cattccagat gcttgatgct ctgctgcgcc
     6121 agtaccgccg ccggaccgcc gatactaccg aggtagaagc cgccgtgttt atgacacgcg
     6181 tcggtaacct gctgactgcg gttacctttc gccagcatga tcatgctgcc gccgtgggat
     6241 tgcagcagat ccacgtagga gtccatacga cctgcggtgg ttgggccaag tgaacctgat
     6301 ggataaccgg caggggtttt cgccggaccc gcgtagtaga tcgggtgatc tttgatgtac
     6361 tgcggcagtt ctttaccggc gtcaatcagc tctttcagct tggcgtgtgc aatatctcga
     6421 cccacaataa tggtgccggt gagcgacaaa cgggtggata ccgggtattg cgaaagctgg
     6481 gcgaggatct ctttcatcgg gcggttaagg tcaactttca ccgcttcgcc ttcaccggcc
     6541 tggcgcagtt cttgtggaat gtactggcct gggttgtgtt ccagtttttc gatccagata
     6601 ccttcgcggt tgattttcgc tttaatgtta cggtctgcgg agcaggagac gcccatgcct
     6661 accgggcagg atgcgccgtg acgtggcaga cggataacgc gaatgtcgtg cgcgaagtat
     6721 ttaccgccaa actgcgcgcc aagaccgagt ttctgggcct cttccagcag ttcctgttcc
     6781 agctggacat cgcggaacgc ctgaccatgt tcgttccctt ccgtcggcag ttcatcgtaa
     6841 tagtgagcgc ttgctaactt gacggttttc aggttggttt ccgcagacgt accgccaatc
     6901 acaaacgcga tatggtacgg cgggcaagct gccgtaccga gggtacgcat tttctcgacg
     6961 aggaagtttt tcagtttgcc gggcgtcagc agggctttgg tttcctggta gagatacgtt
     7021 ttgttggcag agccgccgcc tttagcgaca cagaggaatt tgtactcatc gccatctacc
     7081 gcgtacaggt cgatttgcgc aggcaggttc gtgccggtgt tgacctcttt atacatgtcc
     7141 agcggcgcgt tctgtgaata gcgcaggtta tcttcgatat aggtgttata gacgcctttc
     7201 gacagcgctt cttcatcacc gccaccggtc cacacgcgct gacctttttt accgacgatg
     7261 atcgcggtgc cggtatcctg gcaggtcggc agcacgcctt tggcggcgat ttcggagttt
     7321 cttaagaatt gcagcgccac gtacttgtcg ttttcgctgg cttctggatc gtgaagaata
     7381 gccgcaacct gtttctggtg tgctgggcgg agcatgaaag aagcgtcgtg aaaggcttgc
     7441 tgcgccagca gggtcagggc ttctggttcc actttcagga tggtttcgcc gtcgaagtcg
     7501 gcaacgctaa cgtaatcgga agtgagtaga tagtattcgg tattgtcttt ccccatcggg
     7561 aaaggtgcct ggtagataaa gggtttgttt gacatagctt ccagcctgta actctgtatt
     7621 tgttattcga aaatggcgtg ccgcgtagtc acgggcggca cgcaaagtgc atttataaga
     7681 acccgtacat cgcggcgaag atccagccga agacgcacga tacgctcaca ccaatcaacc
     7741 ccggcagaat aaagctgtgg ttgatgacga agcgaccgat gtgggtggtg ccggaacggt
     7801 caaactgaat cgctgccaga tcgctcggat aagtcggcag gatgtaataa ccgtagcaag
     7861 ccggtgctga agccacgatg tatgccggat caacgccgat cgccagcgcg accggaacaa
     7921 tcgccgccag cgccgcagcc tgagagttta caaacttgga aaccagcagc agaacaatgg
     7981 cataggccca cggatactct ttcaccattt cacccagtac gccctgaatt tcagacatat
     8041 gcgcaccgaa catggtttct gccatccatg cgataccgta caccgccacg atggcgatca
     8101 taccggaacg gaagacttcg ttttttgaga tagacgcggg attggttttg gtcaggataa
     8161 taatcagcgc cccggtcagc agcataaaca tctgaataac cagtaccatc gacagcggtt
     8221 tgccgccgaa ggatggacgc aggtccgaat cagcaccaag aagggcgact acagcgattg
     8281 ccccgaggaa gatccacatt gccagccagt tgcttttcgg cagtttttta tccagcagcg
     8341 tcgcggtatc accgtaaaca tactcacggt tttccggtac ggagatgaat ttctggaact
     8401 cttcgtcttt atccagatct ttaccgcgga accagctgaa gataccgatc gccaggatac
     8461 cgattaacgt cgatggaatg gtgattgcca gcaggtcgag gaactcaaga tggcgaccat
     8521 caaaggtgac attacccagc atcgcaacca gagacacgac tgcaaccgac accggactgg
     8581 cgataatccc catctgtgca ccgatagaac ttgccgccat tggacgttcc ggacggatgt
     8641 tgttcttaat agcaacgtcg tagatgatcg gcagaatggt gtaaaccaca tgacccgtac
     8701 cgcaaagaat ggtcagcgta caggtcacaa acggcgcgac aattgagaca tatttcgggt
     8761 tgcggcgcag cagcttctcg gcaatttgca gcatgacatc aagaccgccc gaagcttgca
     8821 aggtcgccga tgccgccacc accgcaatga taaccagcat gacatcaacc ggtggtttac
     8881 ctggctgaag gtggaagacg aagaccagaa tgaccagacc gataccgcct aataaaccca
     8941 gcgcgatacc accctttctg gcaccataaa acagacatat cagtattatg ataagttgga
     9001 tagtaaataa catgtgtgaa ccctcgcgat aatcctattt aaatttttgc tgaatagatc
     9061 acagtcacgt tctgttttgt atgaactgtt ttcagagcct gactaaatat ccgtttggtc
     9121 gttaccggct ttagcaaata cctcacagtg aatattggct gattaaagca gcggcaaatt
     9181 tatgcactgt gataagcggc tttttcagag agaaaaactc tctggcgatg ggtttatagt
     9241 ttgtactgcg cagatgttaa tgttttgttt gctgttatct tattgatatt tatcggt
//
LOCUS       DAZFLD010000183         9278 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.184, whole genome shotgun sequence.
ACCESSION   DAZFLD010000183 DAZFLD010000000
VERSION     DAZFLD010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9278
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.184"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            87..614
                     /locus_tag="ACHKBL_003856"
     CDS             87..614
                     /locus_tag="ACHKBL_003856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFO5820659.1"
                     /translation="MSARGINKVILVGRLGNDPEVRYIPNGGAVANLQVATSESWRDK
                     KTGEMREQTEWHRVVLFGKLAEVAGEYLRKGAQVYIEGQLRTRSWEDNGITRYVTEIL
                     VKTTGTMQMLGSAPLQNAQAQPQPQQNGQPQSADATKKGGAKTKGRGRKAAQPEPQPQ
                     PPEGEDYGFSDDIPF"
     gene            672..905
                     /locus_tag="ACHKBL_003857"
     CDS             672..905
                     /locus_tag="ACHKBL_003857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 domain-containing protein"
                     /protein_id="HFO5820660.1"
                     /translation="MSEYFRILQGLPDGPFTRKQAEAVAAQYRNVFIEDDNGEHFRLV
                     VRNNGAMVWRTWNFEDGAGYWMNHVIRDFGILK"
     gene            969..2927
                     /locus_tag="ACHKBL_003858"
     CDS             969..2927
                     /locus_tag="ACHKBL_003858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HFO5820661.1"
                     /translation="MSVTESKAKTERKSSRKPAKTQETVLSALLAQTEDVSVPLASLI
                     KSPLNVRTVPYSAESVSELAESIKGVGLLQNLVVHALPGDRHGVAAGGRRLAALNMLA
                     ERGIIPADWPVRVKIIPQELATAASMTENGHRRDMHPAEQIAGFRAMAQEGKTPAQIG
                     DLLGYSPRHVQRMLKLADLAPVILDALAEDRITTEHCQALALENDTARQVQVFEAACQ
                     SGWGGKPEVQTIRRLVTESEVAVAGNSKFRFVGADAFSPDELRTDLFSDDEGGYVDCV
                     ALDAALLEKLQAVAEHLREAEGWEWCAGRMEPVGSCREDAGTYRSLPEPEAVLTEAEE
                     ERLNELMTRYDALENQCEESDLLEAEMKLMRCMAKVRAWTPEMRAGSGVVVSWRYGNV
                     CVQRGVQLRSEDDAAADADRTEQVQEKASVEEISLPLLTKMSSERTLAVQAALMQQPD
                     KSLALLAWTLCLNVFGSGAYSKPAQISLECKHYSLTSDAPSGKEGAAFMAMMAEKARL
                     AALLPEGWSRDMTTFLSLSQEVLLSLLSFCTACSIHGVQTRECGHTSRSPLDTLESAI
                     GFHMRDWWQPTKANFFGHLKKPQIIAALNDAGLSGAARDAEKMKKGDAAEHAEFHMKD
                     NRWVPGWMCTPRPQMDATEHTTNLADAA"
     gene            2982..3416
                     /gene="psiB"
                     /locus_tag="ACHKBL_003859"
     CDS             2982..3416
                     /gene="psiB"
                     /locus_tag="ACHKBL_003859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000845953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugation system SOS inhibitor PsiB"
                     /protein_id="HFO5820662.1"
                     /translation="MKTELTLNALQSMNAQEYEDIRAAGSDMRRNLTHEVMREVDAPA
                     NWMMNGEYGSEFGGFFPVQVRFTPAHERFHLALCSPGDVSQLWMLVLVNCGGQPFAVV
                     QVQHIFTPVAISHTLALAATLDAQGYSVNDIIHILMAEGGQA"
     gene            3413..4132
                     /locus_tag="ACHKBL_003860"
     CDS             3413..4132
                     /locus_tag="ACHKBL_003860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001584594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid SOS inhibition protein A"
                     /protein_id="HFO5820663.1"
                     /translation="MSARSRALIPLSAEQQAAMQAVAVTEQRRRQGRTLSAWPYASAF
                     FRCLNGSRRISLTDLRFFAPALTKEEFHGNRLLWLAAVDKLIESFGELCVLPLPSDAG
                     HRLFPSVPFREGERRRQKTTLTEQKYSRQREREAERRELEYQTCFAQAQIDLAFHTPA
                     TVGSWLSRWSGVVEEHDLETIFWGWCGRFPSLSSFDRFFWQEEPLWRLIFEAGEAGRG
                     APVQVRALEQWMIPNKLENTI"
     gene            4129..4725
                     /locus_tag="ACHKBL_003861"
     CDS             4129..4725
                     /locus_tag="ACHKBL_003861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000978013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5820664.1"
                     /translation="MMKSDEKYQVPVWMRPLLPLLCNTGGNDPEELLNDTETTASANI
                     VRYVLIVAVRSQIDLLQLLYRKGLLRTEIPGGFSPEEAQELLDNLVRSHISKALSGER
                     MAARDRNADLAWIRQQLVDAAWFVRATLEAHGMGVGNESPSAPPETMPDIQTRELVML
                     IKRLASSLKAVKPDSSVVRAAQDWLCDRKLVDITDILR"
     gene            5186..5686
                     /locus_tag="ACHKBL_003862"
     CDS             5186..5686
                     /locus_tag="ACHKBL_003862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HFO5820665.1"
                     /translation="MSVVAPAVYVGTWHKYNCGSIAGRWFDLTTFDDERDFFAACRAL
                     HQDEADPELMFQDYEGFPGNMASECHINWAWVEGFRQARDEGCEEAYRLWVDDTGETD
                     FDSFRDAWWGEADSEEAFAVEFVSDTGLLADVPETVALYFDYEAYARDLFLDSFTFID
                     GHVFRR"
     gene            6416..6850
                     /locus_tag="ACHKBL_003863"
     CDS             6416..6850
                     /locus_tag="ACHKBL_003863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000218847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="post-segregation killing protein PndC"
                     /protein_id="HFO5820666.1"
                     /translation="MTVSSTISVFCRDGVFRTVYCHLHGEPTWNGRILHTHYATGQQA
                     EALVEHGDIRCLGPRCDKPAGHTLQNPVDGVTAYYGRDSGFRMDSEAREYRSFMEAIA
                     TESTEEVRFHYVFIDGYWKVMYRTPEGWKMKALALALRRCPE"
     gene            6943..7209
                     /locus_tag="ACHKBL_003864"
     CDS             6943..7209
                     /locus_tag="ACHKBL_003864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5820667.1"
                     /translation="MRPSIIFATAEYVKRLREECLRENKPLHRHTRFRRQELAPDEIN
                     PDVLAMGGHIARRCSERKRVRIPAMKVSEWGHLLRALEIERVCH"
     gene            7399..7788
                     /locus_tag="ACHKBL_003865"
     CDS             7399..7788
                     /locus_tag="ACHKBL_003865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein CbiX"
                     /protein_id="HFO5820668.1"
                     /translation="MKSCFDTDKDFQMSSDEVTFNADSVRSKLELLSEELMHRCGLVY
                     ELYQRRLSAAIDNFVATLPTEQHLLVLELAREEFDYLSAEEIAEEIRRDAERGYCCHG
                     FDRNCCPLGCGDLDDDCCDAADLMEEP"
     gene            7785..8747
                     /locus_tag="ACHKBL_003866"
     CDS             7785..8747
                     /locus_tag="ACHKBL_003866"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004536 - DNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HFO5820669.1"
                     /translation="MSKKKNTTTPTPHDAAFRSFLANPDVARDFLELHLPAEYRQLCD
                     LSTLKLEPASFVEPDLHQYASDILWSVKTTGGKDGYVYTLIEHQSTENLYMPFRMLRY
                     SVAAMQRHLEQYKTLPLVIPVLFYHGERSPYPYSMNWLDCFEDPALAAKIYTKPFPLV
                     DITVVDDNEIMNHRRMAALTLLMKHIRHRDMMELLDKLPQVMVEISDEQVRVLIHYIV
                     NAGDSVSPEFMRALAERLPQHEDKLMTIAERLEQQGIQKGIQQGIQQGIQQGIQQGIQ
                     QGIQKGRMEEKQDIARQLQKTGMPSAQICQITGLSEAELKNITH"
     gene            8809..9003
                     /locus_tag="ACHKBL_003867"
     CDS             8809..9003
                     /locus_tag="ACHKBL_003867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000150316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5820670.1"
                     /translation="MTIAERLIQKGFDEGFKQGFKQGALEVAREIACRLRDIGWPPER
                     IQEATGLSGEELKKLFPDEQ"
     gene            complement(9034..>9278)
                     /locus_tag="ACHKBL_003868"
     CDS             complement(9034..>9278)
                     /locus_tag="ACHKBL_003868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003980153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFO5820671.1"
                     /translation="WELRIWRKSMLWSREKAAREFGVTQRTWHAWENAEQVDVTVWRT
                     TQALSVRDLLPHMQGMRKADIIRRLENELGETAEDV"
BASE COUNT         2033 a         2485 c         2773 g         1987 t
ORIGIN      
        1 aggagctttc gctcctctga aggggcacat gaacgccccg tctccggtat ccggtttatc
       61 tcacatcttt cagtcaggag gttctcatga gtgcacgtgg tatcaacaag gtcatcctcg
      121 tcgggcgtct gggcaatgat ccggaggtcc gttacatccc caacgggggc gcagtggcaa
      181 acctgcaggt ggccacgtca gaaagctggc gcgacaaaaa gacgggggag atgcgggagc
      241 agacggaatg gcaccgcgtg gtgctgttcg gcaagctcgc ggaagtggct ggtgaatatc
      301 tgcgcaaggg cgcgcaggtc tatatcgagg ggcaactccg aacccgtagc tgggaagata
      361 acggtatcac ccgttacgtc actgaaattc ttgttaagac cacgggcacc atgcagatgc
      421 tggggagtgc accactgcag aacgctcagg cacaaccgca gcctcagcag aatggtcagc
      481 cacagagtgc tgacgcgacg aaaaaaggtg gcgcgaaaac gaaaggccgt ggacgtaagg
      541 ccgcgcagcc cgagcctcag ccgcaaccgc cggaggggga ggattacggg ttttcagacg
      601 atatcccgtt ctgactggcc tgactgtgat aaccgccccg ccctgcgcgg ggcatcaccg
      661 gagatatgag gatgagcgaa tatttcagaa tacttcaggg actgcctgac ggtcctttta
      721 cccgcaaaca ggccgaagcc gttgccgcac agtaccggaa cgtctttatc gaggatgaca
      781 acggagaaca ttttcgcctg gttgtccgta ataacggtgc aatggtctgg cgtacctgga
      841 attttgagga cggtgccggg tactggatga accatgtcat cagggatttc ggcattctta
      901 agtaagagag gtgccggacg ggtaggcgtc cggcagacat acaaccaccc cggtcaaagg
      961 agaaatatat gtcagttaca gagtctaagg caaaaacaga gcgcaaatcc agccgtaaac
     1021 ctgcaaaaac gcaggaaact gtcctgtctg ccctgctggc gcagacggag gatgtgagcg
     1081 ttccgctggc ttcactgatt aaatcaccgc tgaatgtgcg cacggtgccg tattctgcgg
     1141 agtccgtcag cgaactggct gagtccatta agggcgtcgg cctgctgcag aatctggttg
     1201 ttcatgccct gccaggtgac cgccacggtg tcgccgcagg tggtcgccga ctggcagcac
     1261 tcaacatgct ggcagagcgt ggcatcattc cggctgactg gcctgtccgc gtgaaaatta
     1321 ttccgcagga actggcgact gccgcatcaa tgactgaaaa cggtcatcgt cgggatatgc
     1381 accctgccga acagattgcc ggattccgcg caatggcgca ggaaggcaaa acacctgcac
     1441 aaatcggtga tttgctgggc tattcacccc gccacgttca gcgaatgctg aaactggcag
     1501 accttgcacc tgtcatcctt gatgcgctgg cagaagaccg catcaccaca gagcactgtc
     1561 aggcgctggc gctggagaac gacaccgcgc gtcaggtgca ggtgtttgaa gccgcctgcc
     1621 agtcaggatg gggcggtaaa ccggaagtgc agaccattcg tcgtctggtg accgaaagtg
     1681 aagtggcggt ggcggggaac agtaaattcc gcttcgtggg ggctgatgcc ttctcgccag
     1741 atgaactgcg cactgacctg ttcagcgacg acgagggtgg ctatgtggac tgcgtggcgc
     1801 tcgatgccgc cctgctggaa aaactccagg ctgtcgctga acaccttcgg gaagccgaag
     1861 gctgggaatg gtgcgccgga cgtatggagc ctgtcggttc ctgccgtgag gatgccggaa
     1921 cataccgcag cctgccggag ccggaagcgg tgctgacgga agctgaagaa gaacgcctga
     1981 acgaactgat gacgcgctac gacgcgctgg aaaaccagtg tgaggaatct gacctgctgg
     2041 aagcagaaat gaagctgatg cgctgcatgg cgaaggtcag ggcgtggacg ccggagatgc
     2101 gtgccggaag cggtgtggtg gtgtcctggc gttatggcaa cgtgtgtgtc cagcgtggtg
     2161 tgcagttgcg cagcgaggat gacgcggctg ccgacgctga ccgcacggaa caggtgcagg
     2221 agaaagcgtc agtggaggaa atcagtctgc cactgctgac gaaaatgtct tccgaacgca
     2281 cgctggcagt ccaggcggca ctgatgcagc aaccggacaa atctctggca ctgctggcat
     2341 ggacgctctg cctgaatgtg tttggcagcg gagcgtacag taaaccagca caaatcagcc
     2401 tggaatgtaa acattattcg ctgaccagcg atgcgccgtc agggaaggaa ggtgccgcgt
     2461 tcatggcgat gatggcagaa aaagcccgtc ttgcggccct gttaccggag ggatggtcac
     2521 gggacatgac gacgttcctt tcactcagtc aggaggtgct gttatccctg ctcagtttct
     2581 gcaccgcgtg cagcatccac ggcgttcaga cccgcgagtg tggtcacacg tcacgcagcc
     2641 cgctggacac gctggagagc gccatcggtt ttcacatgcg cgactggtgg cagccgacaa
     2701 aagcaaactt cttcggacac ctgaaaaagc cgcagattat cgcagccctg aatgatgccg
     2761 gactgtccgg tgctgcgcgg gacgcggaga aaatgaagaa aggcgatgcg gctgaacatg
     2821 cagagttcca tatgaaagac aaccgctggg tgcctggctg gatgtgtaca ccacgtccac
     2881 agatggatgc cactgaacac accactaacc tggctgatgc cgcctgatga acaaccacac
     2941 cgccccgccg gagacggggc ggcagcaagg gagataccgt gatgaaaaca gaactgaccc
     3001 tgaatgccct gcagtccatg aacgcacagg aatatgaaga tatccgggct gccggaagcg
     3061 atatgcgccg taatctcact cacgaggtga tgcgtgaagt ggacgcaccg gctaactgga
     3121 tgatgaatgg cgagtatggc agtgagttcg ggggcttttt ccccgtccag gttcgtttca
     3181 cgccagccca cgaacgtttc cacctggcat tatgttcgcc gggagacgtc tctcagctct
     3241 ggatgctggt tctggtgaat tgtggtggac agcctttcgc cgtcgttcag gtgcaacata
     3301 tcttcacgcc tgtcgctatc agtcacacgc tggcgcttgc cgcgacactt gatgcgcagg
     3361 ggtacagtgt taacgacatc atccatatcc tgatggcaga aggaggtcag gcatgagcgc
     3421 acgctcacgg gcactgatcc ccctcagcgc agaacaacag gccgccatgc aggctgtggc
     3481 tgtcaccgaa caacgtcgtc gtcagggacg cacactttca gcatggcctt atgccagcgc
     3541 tttctttcgc tgcctgaatg gcagtcgccg gatttcgctg accgatctcc gcttttttgc
     3601 tcctgcgctg acgaaggagg aatttcatgg caaccgtctc ctgtggctgg ctgccgtgga
     3661 taaactgatt gaaagttttg gtgaactctg tgttcttccc ctgccatccg atgcggggca
     3721 ccgtctgttc ccgtccgttc cttttcgtga aggtgagcgg cgtcgtcaga aaaccacgct
     3781 gacagagcag aaatacagcc gccagcggga acgtgaggca gaacgacggg aactggaata
     3841 ccagacatgt tttgctcagg cgcagattga ccttgcgttt catactcccg ccacggtcgg
     3901 aagctggttg tcccgctggt ctggtgttgt tgaggagcat gatctggaaa cgattttctg
     3961 ggggtggtgc gggcgttttc cgtcactgtc atcatttgac cggtttttct ggcaggagga
     4021 accactctgg cggctgattt ttgaagccgg tgaggccggt cgtggtgcac cggtacaggt
     4081 acgtgcactt gagcagtgga tgatcccgaa caaactggag aacacaatat gatgaaatca
     4141 gacgaaaaat atcaggttcc cgtgtggatg cgacctctgt tgccgttgct ctgcaatacc
     4201 ggtggtaatg atccggagga actgctgaat gatacagaaa ccaccgccag tgcgaatatt
     4261 gtccgttatg tactgatagt tgcagtgcgg tcgcagattg atctgctgca gctactgtac
     4321 aggaaaggac tgttgcgcac agagatacca ggtggctttt caccggaaga agcgcaggaa
     4381 ctcctggata atctggtgcg cagccatatc agcaaggcgc tgtcgggtga gcgaatggca
     4441 gcccgtgaca gaaatgccga tctggcctgg attcgccagc aactggtcga tgctgcctgg
     4501 tttgtccgtg ccacactgga agcgcatggg atgggcgtcg gaaatgagag tccctctgct
     4561 ccgccggaga caatgccgga catacagaca cgggaactgg tgatgctgat caaacgactg
     4621 gcatcttctc tgaaagcggt gaaaccggac agtagcgtag tgcgtgcggc gcaggactgg
     4681 ctgtgcgaca gaaaacttgt ggatatcaca gatattctcc ggtgaaccag gcatgtatat
     4741 ttttcgcagg gacacgccga tcatgatcgt ttcatatcag gggtggtctg gaggtcatgc
     4801 ggcgtgtcct ctgcactcgc cggaataagg aagtcgccgg cggctccgct tttacccggc
     4861 catgcggggc atggccttgt gggttttcag ttatgtggcc tcagcgtcgt gtgcgggctg
     4921 tgccgtgcct ccatcttagc cggctggcgg ggatgcaagg gtaaaatgca cgcggcggtc
     4981 acccggtccc tccttcccgt ctgccgtgat tttccggtcg ttcacccgct cagatttcgc
     5041 ggtctcaccc tccaactccc gccagccgtg tgcgtcaggc tgcggcttcc cttgcgccct
     5101 gtgcatcccc gccttatcgc ccggctttta tggaggcacg gcaccgcccg gtgccgcaga
     5161 acatgtgact atggaggatt cgggaatgtc tgttgttgca cctgctgtat acgttggaac
     5221 ctggcacaaa tacaactgtg gaagcatcgc cggacgctgg tttgacctga ccacgtttga
     5281 tgatgagcgc gactttttcg ccgcctgccg tgctcttcac caggatgaag ccgatcctga
     5341 actgatgttt caggattatg agggattccc ggggaatatg gcctctgaat gtcatatcaa
     5401 ctgggcctgg gttgaaggct tccgccaggc acgggatgaa ggctgcgaag aggcttatcg
     5461 tctctgggtg gatgataccg gtgagacgga ttttgacagc ttccgcgatg cctggtgggg
     5521 cgaggctgac agtgaggagg cttttgcggt tgagttcgtc agtgataccg gcctgctggc
     5581 tgacgtgccg gagacggtgg cgctctattt tgactatgag gcgtatgcgc gggatttatt
     5641 cctggactcc ttcaccttta ttgacggtca tgtgttccgt cggtgattta ctccccgctc
     5701 cggcgggggt atggcctgcc aggcccccgg aaaaccctgg caggcttacg cctgcctgaa
     5761 atgcaggagg gccgcgcctg cggcgctgcc cttcctgccc gtcatggagt gaggcatttt
     5821 tctgttatgt cgtttctgtt gttctgattt ccggaggcgg ggacaccttc ggctcccggc
     5881 tgcctggcgg catccggtcg tcaggcccga ctccgacagg cagctgctcg cgcacccgcc
     5941 agtctccgcc gaacccgctg gcgcggtttc gggctgccgc tacggtccga tgtctgaaaa
     6001 acctggcttg cagcgaccgt tccggtcgcg ccgcctctgt cgcgccgtga tctgcgctcc
     6061 ctgcacacct gacggtgtgt aaggaaactg cgcctgatgg cgcagttgtc tgcccttccg
     6121 ccgccacccc tgcggcctgt ttcccgctgt ggactttccg ggggctgaag cggccacaac
     6181 acgtgtcagc cgtccagggg gagatgtacc agccacaaaa ctttttgccg caagcgtcaa
     6241 aaacttttgc gtctgccccc ggacaactgc cccgtgccgt ggctgtcgct gccccatcgg
     6301 aaaggtccac tgcggggaga aaccccgaag aagtggacgg cagaaggggc tttcgcccct
     6361 ccgggggagc aaccggtcgg cccggcgacc tgattcagca gaaggagacg ttttcatgac
     6421 agtcagcagc acgatttccg ttttttgccg cgacggggtg ttccgtaccg tttactgcca
     6481 cctgcacggt gagccgacct ggaacggtcg catcctgcat acccactatg ccaccggtca
     6541 gcaggccgaa gccctggttg aacacggtga tatccgttgc ctcggtcccc gttgcgacaa
     6601 acccgccggg catacgcttc agaacccggt ggacggtgtg acggcttatt acggacgtga
     6661 cagtggtttc cggatggaca gtgaggcgcg tgagtaccgt tctttcatgg aggctattgc
     6721 cacggaaagc acagaagagg tgcgcttcca ttatgtgttc atcgacggct actggaaggt
     6781 gatgtaccgc acgccggaag gctggaagat gaaagcgctc gcgctggcac tgcgtcgctg
     6841 tccggaatga aaaaaagcag ggcgataaac cctgcctttc tccgccggcg cttccccgta
     6901 cggaagtccg gcatctcatt cactaactat ggagattatg ccatgagacc atcaattatc
     6961 ttcgcaaccg ccgagtatgt aaagcgtctg cgtgaagagt gcctgcggga gaacaaaccc
     7021 ctgcaccgcc atacccgctt cagacgccag gaactggcac cggatgagat taacccggac
     7081 gtcctggcga tgggcggtca tatcgccaga cgctgcagtg agcggaagcg ggtgcgtatc
     7141 cccgccatga aagtcagcga gtgggggcac ctgctccgtg cgcttgaaat tgaacgggtc
     7201 tgccactgat taccccctgc cgtctgtctg tgcaggtggc aggatatgtt caggtacttt
     7261 accaggagga accgtgagca agatgaaaaa catcatgacg tctatgtcgc atggtaccgg
     7321 attcctcctg ttccataaaa aacagagaag ggaaacaggt tctctttcat tcagggcccg
     7381 gacgggcagg agataaaaat gaagtcttgt tttgacactg acaaagattt tcagatgtca
     7441 tctgatgaag tcactttcaa tgctgacagc gttcgctcaa aactggaact attatccgaa
     7501 gaactaatgc accgttgtgg tcttgtttat gagctatatc agcgacgttt gagtgctgct
     7561 attgataatt ttgtggctac attgccgaca gaacaacatc tgctggtcct ggaactggcc
     7621 cgtgaggagt ttgattacct ttccgctgaa gagattgcag aagaaatacg ccgtgacgca
     7681 gaacgtggct attgttgtca cggctttgat cgcaattgct gtcctcttgg ttgtggggat
     7741 ttggacgatg attgctgcga tgctgctgat ttgatggagg aaccgtgagt aagaagaaaa
     7801 acaccacaac gcccacgccg catgatgccg cgttccggtc gttcctggcg aaccccgacg
     7861 tcgccagaga ttttctggaa ctgcatcttc cggcggagta ccggcagttg tgcgacctgt
     7921 ccacgctgaa gctggaaccc gcctcctttg ttgagccgga cctgcatcag tacgccagcg
     7981 atatcctctg gagtgtgaaa accaccgggg gtaaagacgg ctatgtttat acgctcatcg
     8041 agcaccagag caccgaaaat ctgtacatgc ctttccgcat gttacgttac agtgtggcgg
     8101 cgatgcagag acatctggag cagtacaaaa cattgccact ggtgattccg gtgctgttct
     8161 atcatggtga gcgcagcccg tacccgtaca gcatgaactg gctggactgt tttgaggatc
     8221 cggcgcttgc ggctaaaata tacacaaagc cgtttccgct ggttgatatc actgtcgttg
     8281 atgacaatga aatcatgaac catcgccgga tggccgcact gacgctgctg atgaagcata
     8341 tccgccatcg tgacatgatg gagctgctgg acaaactccc gcaggtcatg gtggaaattt
     8401 cagatgaaca ggtgcgtgtt ctgattcatt acatcgttaa cgcaggggac tctgtatcac
     8461 cggaatttat gcgggcgctg gctgagcgtc tgccgcagca tgaggataaa ctgatgacta
     8521 ttgccgaacg tcttgagcag caaggaatac agaaaggaat acagcaggga atacagcagg
     8581 gaatacagca gggaatacag cagggaatac agcagggaat acagaaaggc cgcatggagg
     8641 agaagcagga tattgcgcgt cagcttcaga aaacgggaat gccatctgca caaatctgtc
     8701 agatcaccgg actttccgaa gctgaactga agaacatcac tcactgatga gaaccacaaa
     8761 gcccggcaaa atgccgggtt ttttgtcagc aggagcagag caaacagcat gacgattgca
     8821 gaacgcctta tacagaaagg tttcgatgag ggttttaaac aaggttttaa acaaggggct
     8881 cttgaagtag cgcgggaaat agcctgccgg cttcgggata taggctggcc gccggaacgg
     8941 attcaggagg caaccggact ttccggtgaa gaactgaaaa agctgtttcc tgatgagcag
     9001 tagcctggca ttcagccagg ctgtgaatca cgctcacaca tcctctgccg tttcccccag
     9061 ttcattttcc agtcgcctga tgatatctgc cttacgcatc ccctgcatgt gaggcagcag
     9121 gtcacggact gaaagtgcct gggtggtcct ccagacggtg acatccacct gttccgcatt
     9181 ttcccaggcg tgccatgttc tctgtgtcac gccaaattct ctggccgctt tttcgcgtga
     9241 ccagagcatg ctttttcgcc agattcgcag ttcccagc
//
LOCUS       DAZFLD010000184         9243 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.185, whole genome shotgun sequence.
ACCESSION   DAZFLD010000184 DAZFLD010000000
VERSION     DAZFLD010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9243
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.185"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            129..1907
                     /locus_tag="ACHKBL_003869"
     CDS             129..1907
                     /locus_tag="ACHKBL_003869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFO5820672.1"
                     /translation="MELATTQSVLMQIQPTIQRFARMLASVLQLEVEIVDENLCRVAG
                     TGAYGKFLGRQLSGNSRLLRHVLETKTEKVVTQSRFDPLCEGCDSKENCREKAFLGTP
                     VILQDRCVGVISLIAVTHEQQEHISDNLREFSDYVRHISTIFVSKLLEDQGPGDNISK
                     IFATMIDNMDQGVLVVDDENRVQFVNQTALKTLGVVQNNIIGKPVRFRPLTFESNFTH
                     GHMQHIVSWDDKSELIIGQLHNIQGRQLFLMAFHQSHTSFSVANAPDEPHIEQLVGEC
                     RVMRQLKRLISRIAPSPSSVMVVGESGTGKEVVARAIHKLSGRRNKPFIAINCAAIPE
                     QLLESELFGYVKGAFTGASANGKTGLIQAANTGTLFLDEIGDMPLMLQAKLLRAIEAR
                     EILPIGASSPIQVDIRIISATNQNLAQFISEGKFREDLFYRLNVIPITLPPLRERQED
                     IELLVHYFLHLHTRRLGSVYPGIAPDVVEILRKHRWPGNLRELSNLMEYLVNVVPSGE
                     VIDSTLLPPNLLNNGTTEQSDVTEVSEAHLALDDAGGTALEEMEKQMIREALSRHNSK
                     KEVADELGIGIATLYRKIKKYELLNT"
     gene            complement(1947..2426)
                     /gene="xdhC"
                     /locus_tag="ACHKBL_003870"
     CDS             complement(1947..2426)
                     /gene="xdhC"
                     /locus_tag="ACHKBL_003870"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase iron sulfur-binding
                     subunit XdhC"
                     /protein_id="HFO5820673.1"
                     /translation="MNHSETITIECTINGIPFQLHAAPGTPLSELLREQGLLSVKQGC
                     CVGECGACTVLVDGTAIDSCLYLAAWAEGKEIRTLEGEAKGGKLSHVQQAYAKSGAVQ
                     CGFCTPGLIMATTAMLAKPREKPLTITGIRRGLAGNLCRCTGYQMIVNTVLDCEKTK"
     gene            complement(2423..3301)
                     /gene="xdhB"
                     /locus_tag="ACHKBL_003871"
     CDS             complement(2423..3301)
                     /gene="xdhB"
                     /locus_tag="ACHKBL_003871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase FAD-binding subunit XdhB"
                     /protein_id="HFO5820674.1"
                     /translation="MFDFASYHRAATLADAINLLADNPQAKLLAGGTDVLIQLHHHND
                     RYRHIVDIHNLAELRGITLAEDGSLRIGSATTFTQLIEDPVTQRHLPALCAAASSIAG
                     PQIRNVATYGGNICNGATSADSATPTLIYDAKLEIHSPRGVRFVPINGFHTGPGKVSL
                     EHDEILVAFHFPPQPKEHAGSAHFKYAMRDAMDISTIGCAAHCQLDNGNFSELRLAFG
                     VAAPTPIRCQHAEQTAQNAPLNLQTLEAISESVLQDVAPRSSWRASKEFRLHLIQTMT
                     KKVISEAVAAAGGKLQ"
     gene            complement(3312..5609)
                     /gene="xdhA"
                     /locus_tag="ACHKBL_003872"
     CDS             complement(3312..5609)
                     /gene="xdhA"
                     /locus_tag="ACHKBL_003872"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdenum-binding
                     subunit XdhA"
                     /protein_id="HFO5820675.1"
                     /translation="MEAREATATGESCMRVDAIAKVTGRARYTDDYVMAGMCYAKYVR
                     SPIAHGYAISINDEQARSLPGVLAIFTWEDVPDIPFATAGHAWTLDENKRDTADRALL
                     TRHVRHHGDAVAIVVARDELTAEKAAQLVSIEWQELPVITTPEAALADDAAPIHNGGN
                     LLKQSSMSTGNVQQTIDAADYQVQGHYQTPVIQHCHMESVTSLAWMEDDSRITIVSST
                     QIPHIVRRVVGQALNIPWSCVRVIKPFVGGGFGNKQDVLEEPMAAFLTSKLGGIPVKV
                     SLSREECFLATRTRHAFTIDGQMGVNRDGTLKGYSLDVLSNTGAYASHGHSIASAGGN
                     KVAYLYPRCAYAYSSKTCYTNLPSAGAMRGYGAPQVVFAVESMLDDAATALGIDPVEI
                     RLRNAAREGDANPLTGKRIYSAGLPECLEKGRKIFEWEKRRAECQNQQGNLRRGVGVA
                     CFSYTSNTWPVGVEIAGARLLMNQDGTINVQSGATEIGQGADTVFSQMVAETVGVPVS
                     DVRVISTQDTDVTPFDPGAFASRQSYVAAPALRSAALLLKEKIIAHAAVMLHQSAMNL
                     TLIKGHIVLVERPEEPLMSLKDLAMDAFYHPERGGQLSAESSIKTTTNPPAFGCTFVD
                     LTVDIALCKVTINRILNVHDSGHILNPLLAEGQVHGGMGMGIGWALFEEMIIDAKSGV
                     VRNPNLLDYKMPTMPDLPQLESAFVEINEPQSAYGHKSLGEPPIIPVAAAIRNAVKMA
                     TGVAINTLPLTPKRLYEEFHLAGLI"
     gene            6025..6777
                     /gene="actS"
                     /locus_tag="ACHKBL_003873"
     CDS             6025..6777
                     /gene="actS"
                     /locus_tag="ACHKBL_003873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase activator ActS"
                     /protein_id="HFO5820676.1"
                     /translation="MSAGRLNKKSLGIVMLLSIGLLLAGCSGSKSSDTGTYSGSVYTV
                     KRGDTLYRISRTTGTSVKELARLNGISPPYTIEVGQKLKLGGAKNSSTRKSTAKSTTK
                     TASVTPSSAVPKSSWPPVGQRCWLWPTTGKVIMPYSTADGGNKGIDISAPRGTPIYAA
                     GAGKVVYVGNQLRGYGNLIMIKHSEDYITAYAHNDTMLVNNGQSVKAGQKIATMGSTD
                     AASVRLHFQIRYRATAIDPLRYLPPQGSKPKC"
     gene            6856..6929
                     /locus_tag="ACHKBL_003874"
     tRNA            6856..6929
                     /locus_tag="ACHKBL_003874"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6888..6890,aa:Gly,seq:ccc)
     gene            7094..8308
                     /locus_tag="ACHKBL_003875"
     CDS             7094..8308
                     /locus_tag="ACHKBL_003875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001603436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO5820677.1"
                     /translation="MLLSDLAIRRAKPKEKAYTLNDGNGLSLLIEPNGSKGWRLRYRF
                     AGKPKMLSLGVYPEVSLSDARAARDEAKRLIAGGVNPSEVRKAQKREQASRFGNTFEG
                     IAREWYEKRFDRWSASYAEEMIETFEKDVFPYIGGRPIAEIKPMELLAVLSRLNDRGA
                     TEKLRKVRQRCGEVFRYAIITGRAEYNPAPDLASAFAPHKKEHYAYLKVDELPEFFRV
                     FNTYTGSQIVKLGMRLLILTGLRPGELRQAEWQEIDFDNRLWEVPKERMKMRRPHCVP
                     LSEQAIAILEQLKHMTGQYPFIFPGRIQHSKPMSEMAMNVLIRRIGYAGRVTGHGFRH
                     TMSTILHEQGYNSAWIETQLAHVDKNSIRGTYNHAQYLDGRREMLQWYADYMDSLEQG
                     GNVVHGKFGKCG"
     gene            8434..9126
                     /locus_tag="ACHKBL_003876"
     CDS             8434..9126
                     /locus_tag="ACHKBL_003876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001526646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5820678.1"
                     /translation="MPVVNDFPDLSHWFTVQEFTITQAALLLSGIDPLDYPTLEVVKE
                     EKHERWRMAWGFAYGMVTAIRRGVLTPVLCVAYVWGDMRRLTPAEMMDRSNDISMNET
                     IITRYSLQEWVRNERVDYVRPPRSTSVKEVTPSNQPVVIDAESRVINSEETVLLTYGH
                     KSEGLEYVEDAIKNFWATYEPENPQTAPTKTEIEEYLMKMGAGSNMAKAVNLILRPFS
                     LQQVGRRKKKNK"
BASE COUNT         2393 a         2140 c         2387 g         2323 t
ORIGIN      
        1 tcattagaaa gtgataaaaa tcttatgttt ttatcaaagc caatttcgcc tgagttaata
       61 tcatcatgat ctatgaacca tcaacgtctt caatgatcga cttttaagta agtctggaag
      121 gtaaagctat ggagcttgct actacgcagt cagtattgat gcaaattcaa ccgacaattc
      181 agcgttttgc cagaatgctt gccagcgttt tgcagcttga ggttgagatc gttgatgaaa
      241 acttgtgtcg cgtcgccgga acgggcgcgt atgggaagtt tcttggtcgc cagttgagcg
      301 gcaactcacg cctgctccgc cacgtcctgg aaacgaaaac tgaaaaagtt gtgacacagt
      361 ctcgcttcga tcccctttgt gaaggttgcg atagtaaaga aaattgccgc gaaaaagcat
      421 ttctgggtac gcctgtcatt ttacaggatc gttgtgttgg ggtgataagt ttgattgccg
      481 ttacccacga gcaacaagaa catatcagtg ataatttacg cgaattttct gattacgttc
      541 gccatatatc caccattttt gtttcgaaac ttctggagga tcaggggcca ggagataaca
      601 tcagtaaaat attcgcgacc atgatcgata atatggatca gggcgtttta gttgttgatg
      661 acgaaaatcg ggttcagttt gttaatcaga ctgccttaaa aacactcggt gttgtgcaaa
      721 ataatattat tgggaaacct gttcgtttca gaccattaac atttgagagt aattttaccc
      781 atggacatat gcagcatatt gtttcgtggg acgataaaag tgaattaatc attggtcaat
      841 tgcataacat tcagggccga caattatttt taatggcgtt tcaccaatcg cataccagtt
      901 tttctgtagc aaatgcacct gatgaaccgc atattgaaca attggttggc gagtgccgtg
      961 ttatgcggca attaaaacga ctcattagcc gtattgcacc cagcccatcc agcgttatgg
     1021 tggttggcga aagcggcacg ggtaaagaag tcgtcgcccg tgcaatccat aagttgagcg
     1081 gaagacggaa taaacctttt attgctatca actgtgccgc gattccggag cagcttctgg
     1141 agagcgaact gttcggttat gttaaaggcg catttactgg cgcttctgcc aacggtaaaa
     1201 cagggttgat tcaggcggcg aatacgggca cgctgtttct cgatgaaata ggtgatatgc
     1261 cattaatgtt gcaggctaaa ttactgcgcg ctattgaggc acgtgaaatt ctgccgattg
     1321 gtgccagtag cccaatacaa gtcgacattc gcatcatttc tgcaactaac cagaatttgg
     1381 cccagttcat ttccgaaggt aaattccgcg aagatctctt ctaccgactt aatgttatcc
     1441 cgataactct gccaccgctg cgtgaacgtc aggaagatat tgaactactg gtgcattact
     1501 ttttacatct gcatacccgt cgtctgggat cggtttatcc gggcattgct cccgatgtcg
     1561 tcgaaatatt gcgtaagcat cgttggcccg gaaacctgcg cgagttaagc aatttgatgg
     1621 aatatctggt taacgtggtt ccttcaggtg aagttatcga cagcacgcta ttgccgccaa
     1681 atctgctgaa taatggcaca acggagcaaa gtgatgtaac agaggtcagt gaggcgcacc
     1741 tggcactcga tgatgcgggc ggcacggcgc tggaggagat ggaaaagcaa atgatccgcg
     1801 aggcgctttc acgtcataac agcaagaagg aagttgctga tgaactgggc atcggcattg
     1861 ctacgctcta tcgcaagatt aagaaatatg agttgttaaa cacataacct cagtgaatcc
     1921 ggcctgaatt caggccggat atccttttac ttcgttttct cgcaatccag aactgtattt
     1981 acaatcatct gataccccgt gcagcgacaa agatttcccg ccagtccacg acgaattccc
     2041 gtaatggtta atggcttctc gcgtggtttc gccagcattg ccgtggtagc cataatcagg
     2101 cccggcgtac aaaacccgca ctgcactgcg ccggatttcg cataagcctg ctgaacatga
     2161 gaaagttttc cgcctttcgc ttcaccttcc agcgtgcgga tctcttttcc ttcagcccag
     2221 gcggcaaggt ataagcaact gtctattgct gtgccgtcga ccagcaccgt acaggcacca
     2281 cattcaccca cgcagcaccc ttgtttgaca cttagcagtc cttgttcgcg gagtaattcc
     2341 gagagcggcg tgcctggtgc ggcgtgaagc tgaaaaggta tcccgttaat ggtgcattcg
     2401 atggtaattg tttcgctgtg attcattgca attttccccc cgccgcggcg acggcttcgc
     2461 taatcacttt tttggtcatc gtctggatga gatgcagacg aaactcttta ctggcccgcc
     2521 atgacgaacg cggggcgaca tcttgcagga ctgattcgct gatggcttcc agcgtttgca
     2581 ggtttaatgg cgcattttgt gcagtctgtt cggcatgttg gcagcgaatt ggcgttggcg
     2641 cggcaacacc aaatgccagg cgtaattcgc tgaaattgcc gttatccagt tggcaatgtg
     2701 cggcgcagcc aatcgttgaa atatccattg cgtcgcgcat ggcatattta aaatgcgcgc
     2761 tgcccgcgtg ttctttcggc tggggcggaa aatgaaaggc gacaaggatt tcgtcatgct
     2821 caagagacac tttgcccggc ccggtgtgaa agccattaat cgggacgaaa cgaacaccgc
     2881 gcggggagtg gatctccagt ttcgcgtcat aaattagcgt tggcgtggca gaatctgcgc
     2941 tggtggcacc gttgcaaata tttccaccgt aggtagcgac gttacggatc tgcggtccgg
     3001 caatggatga ggccgcagca cataacgccg ggagatgacg ttgagttaca ggatcttcaa
     3061 tgagctgggt aaatgtcgtt gcagagccga tacgtagtga gccatcttcc gccagcgtaa
     3121 ttccccgcag ctccgccaga ttatgaatat caacaatatg gcgataacgg tcattgtgat
     3181 ggtggagctg aatcagtacg tcagtgccac cggcgagcag tttggcctgc gggttgtcag
     3241 ccagcaggtt gatggcatcg gcaagggttg ctgcgcgatg gtaagaagca aaatcaaaca
     3301 tgatgttatc ctcaaatcaa tcctgccaga tggaactctt catataaccg ttttggcgtc
     3361 agcggcagtg tattgattgc aacaccggta gccatcttca ccgcgttacg aatagcagcg
     3421 gcaacaggaa ttattggtgg ctcacccagt gacttatgtc cgtatgcgga ttgcggctca
     3481 ttgatttcga cgaacgcgct ttccagttgt ggcagatccg gcatggtcgg cattttgtaa
     3541 tccagcagat tggggttacg gaccacgccg cttttcgcat caatgatcat ctcttcaaat
     3601 agcgcccagc caatgcccat tcccattccg ccgtgtacct gaccttctgc cagtagcgga
     3661 ttaagaatat ggcccgaatc atgaacgttg aggatgcggt tgatggtgac tttacacagc
     3721 gcaatatcga ccgtcagatc aacaaaggta cagccaaacg ctggtgggtt agtggtggtt
     3781 ttgatggagc tttcggcaga gagctgcccg ccgcgttcag ggtggtagaa ggcgtccatc
     3841 gccaaatctt ttaacgacat taatggctct tccggtcgtt caaccagcac gatatggcct
     3901 tttatcaggg taagattcat cgctgactga tgtagcatga cagcggcatg agcgatgatt
     3961 ttctccttta ataacagtgc cgcactgcgc agcgcaggcg cggcaacata gctctggcgt
     4021 gaggcaaatg caccgggatc gaacggcgta acgtcggtat cttgggttga aataacgcga
     4081 acgtcgctga ccggaacccc cacggtttct gctaccattt gcgagaagac ggtgtcggcc
     4141 ccctgaccga tttctgtcgc gccgctttgc acgttgatgg ttccatcctg attcatcaac
     4201 aggcgcgcgc ctgctatttc tacgccgaca ggccaggtgt tagaggtgta gctaaaacag
     4261 gcgacgccaa cgccgcggcg taaattgcct tgctggttct ggcactctgc acggcgtttt
     4321 tcccattcaa agattttccg gcctttttca agacactccg gcaaccctgc gctgtaaata
     4381 cgtttgcccg tgagcggatt agcatctcct tcgcgggcgg cgttgcgtaa acgaatttca
     4441 acaggatcaa tacctaacgc tgtcgcggcg tcatcaagca tagactcaac ggcaaatacg
     4501 acttgtggcg cgccataacc acgcatcgca ccagccgagg ggaggttggt atagcaggtc
     4561 tttgaactgt aagcgtaggc acaacgagga taaaggtaag cgactttatt tccgcccgca
     4621 gaagcgatgg agtgcccgtg agatgcataa gcgccggtgt tagacagaac atccagacta
     4681 taacctttca atgttccgtc gcggttcacg cccatttgcc cgtcaatagt aaaagcgtgg
     4741 cgggtacggg ttgcgaggaa acactcttca cggctaaggg aaactttcac cggaatgccg
     4801 ccaagcttgc tggtcaggaa tgccgccatt ggctcttcca gtacatcctg tttattacca
     4861 aaaccgccac cgacaaacgg tttgatgact cgtacgcatg accagggaat attcagcgcc
     4921 tgaccaacca cacggcgaac aatgtgcggg atctgggtgc tggaaacgat ggtaattcgc
     4981 gagtcatcct ccatccatgc cagcgatgtc acgctttcca tatgacaatg ttgaataacg
     5041 ggagtctgat agtgcccctg cacctggtaa tcggcggcat cgattgtttg ttggacatta
     5101 cccgtcgaca tcgagctttg ttttagtaaa ttgccgccgt tatggattgg tgcagcgtca
     5161 tctgccagcg ccgcttctgg cgtggtgata acgggtaatt cttgccactc aatgctgacc
     5221 aattgcgccg ctttttctgc cgtgagttca tcacgggcca cgacgatggc aacggcgtca
     5281 ccatgatgac gaacatggcg agttagcagt gcgcgatcgg cggtatcgcg cttgttttcg
     5341 tcaagtgtcc aggcatgccc agctgtagcg aatgggatat caggcacatc ttcccaggta
     5401 aaaatcgcca gtacgcccgg taaacttctg gcttgttcat cattaatact tatggcataa
     5461 ccatgtgcga tagggctacg tacatatttc gcgtaacaca tgcccgccat aacataatcg
     5521 tcagtatatc gtgcccgccc ggtgacctta gcaatggcat ctacgcgcat gcatgattca
     5581 cctgtagcgg ttgcttcccg cgcttccata gaaatcccct cgatttgaac atttggttct
     5641 tatggcaaag atttagtcta agcaggcaag atttacgcca gaaacgacga taagcgttaa
     5701 gggaggaagg aagtgtgatg atgttaaaaa aacacagata tgatacgaaa caggaccctg
     5761 aataggggca gtgatacgta tcaatatgag tggcgataaa atcaatgtga taaattggga
     5821 ttattacgca tcaggatggg aaatagggta aatgagtcag aaaattcgat acatctacag
     5881 tgaatatttt gctgatgata cttccctttg ccaaatgaaa ttaacctctt tctctattac
     5941 cgcgtttttc cagaagcaag agattgggtg tcaagaggct ggctgctatg atatggcgtc
     6001 ttgtttttta agcgaggaaa gattttgagt gcgggacgcc tgaataaaaa atctctgggt
     6061 atcgtgatgt tgttatcgat tggactgctt ttggcgggct gctcgggtag caaatcatcc
     6121 gatacaggaa cgtattccgg ctccgtttac accgtgaaac ggggggatac gctatatcgt
     6181 atttcgcgca ccacgggaac tagcgtaaaa gagctggcgc gactgaacgg catttccccc
     6241 ccttacacca ttgaagttgg tcagaaacta aaactgggtg gggcgaaaaa tagtagtaca
     6301 cgtaaatcaa ccgccaaatc aacgaccaaa accgcatcgg ttacaccgtc atcagcagta
     6361 ccgaaatctt cctggccgcc agtagggcaa cgttgttggt tatggccaac gacagggaaa
     6421 gttatcatgc cgtattcgac agctgatggc ggcaataaag ggattgatat ctcagctcca
     6481 cggggtacac ctatttacgc cgcgggtgca ggaaaggtgg tgtatgtggg caaccagctg
     6541 cgtggctacg gtaatctcat catgattaaa cacagtgaag attacattac ggcttacgcc
     6601 cataatgaca cgatgctggt aaataatggg caaagcgtga aggctgggca aaaaatcgcc
     6661 actatgggga gcactgatgc ggcatcggtt cgcctgcatt tccagattcg ttaccgtgca
     6721 acggcaattg atccgctacg ttacttgccg cctcagggca gcaagccaaa atgctgatgg
     6781 cgaattaatc agcagtcagc agcgcggcac ttgctaagga gagcgtaagg tttataatgc
     6841 cttacgcatc tcgaagcggg cgtagttcaa tggtagaacg agagcttccc aagctctata
     6901 cgagggttcg attcccttcg cccgctccaa acaaacatct accaacgtct actaaagtct
     6961 accaatcccc cgaatatcaa ggcctccagc gattttcctc gtctacaacc gtctacagtc
     7021 atctacctaa atctacacac agaagtgtat agtaatgcgt ataggacaaa atcgtttttt
     7081 gggggctata cacatgttgc tttcagacct tgctatccgt cgcgcgaagc caaaagagaa
     7141 agcctataca ctcaatgacg gcaacgggct ttcgttactc attgagccta acggttcgaa
     7201 gggctggcga ttgcgataca ggtttgcagg taagcccaaa atgttgtcgc tgggtgttta
     7261 tcctgaggtg tccctttctg atgccagggc ggcaagggat gaagccaaaa ggcttatcgc
     7321 tggtggagtt aatccgagtg aggtcagaaa ggcccaaaag cgagaacagg cgagcagatt
     7381 cggtaatacc tttgagggta tcgcgcgtga gtggtatgaa aagcgttttg acagatggtc
     7441 cgcgtcatat gcagaggaaa tgatagagac gtttgaaaag gacgtttttc cgtacattgg
     7501 aggcaggccc atcgcagaga ttaagccgat ggaattgctc gcggttctct ctcgtcttaa
     7561 tgaccgtggc gcaactgaaa aattgcgtaa ggttcgacag cgatgtggcg aagtgttccg
     7621 gtacgcaata atcaccggac gcgctgaata taaccccgct cctgatctgg caagcgcatt
     7681 tgcaccacac aaaaaagagc attacgcgta tctgaaagtt gatgagctac cggaattttt
     7741 ccgcgttttt aatacgtata ccggaagcca gatcgtcaag ctgggtatgc gattattgat
     7801 tctgacagga ctccgtccag gggaattacg acaggcagag tggcaggaga ttgattttga
     7861 taatcgtctg tgggaagtgc cgaaagaacg catgaagatg cgccgtcctc attgtgtgcc
     7921 gctttcagaa caggcgatcg ctattctgga acagttaaaa cacatgacgg ggcagtatcc
     7981 gtttattttc cctggtcgaa tccagcacag taaaccgatg agtgaaatgg cgatgaacgt
     8041 gcttattcgt cgtattggtt atgcagggcg tgttactggt catgggttcc ggcacaccat
     8101 gagtacgatt ttgcacgagc aggggtataa ctccgcatgg atagaaacgc aactggcgca
     8161 cgtcgataaa aactcgatcc gtggcacata caatcacgcg caatatctgg acggacggcg
     8221 ggagatgctc cagtggtacg ccgactatat ggactcactg gaacagggcg gaaacgtggt
     8281 acatggcaag ttcggaaaat gtggatgact ggttgagtat acagtagtag actttgagcg
     8341 actaaaaact gctgtgtcta gggtcgctcc tgaaaacccg tacacctctg cgggctggca
     8401 cagccaccac tacagagagt gcgaggtgtg cacatgccgg ttgtgaatga tttccctgac
     8461 ttaagtcatt ggtttacagt gcaggagttt actattactc aagctgcttt attgctgtca
     8521 ggtattgatc ctctcgacta cccaactctg gaggtagtca aagaggaaaa acacgaacga
     8581 tggagaatgg catgggggtt tgcttatgga atggttactg caattcgacg cggtgtctta
     8641 actcccgttc tgtgcgttgc gtatgtttgg ggggatatgc gtcgtttaac cccagcagag
     8701 atgatggatc gttcaaatga tatatccatg aacgaaacaa ttattacccg ttattcatta
     8761 caggaatggg taagaaatga gagggtcgat tatgtaagac ctcctcgctc cacatcggtt
     8821 aaggaagtta ccccatctaa ccaaccagta gtaattgatg ctgaatcgcg cgtgattaat
     8881 tctgaagaaa cagtattgct gacttatggt cataagagcg aaggtcttga atatgtagaa
     8941 gacgcaataa aaaatttttg ggcaacatat gaaccagaga atccacagac agcgcctaca
     9001 aaaacagaga tcgaggaata cctaatgaaa atgggggctg gaagcaacat ggcaaaagcg
     9061 gtaaatctga tactacgccc attcagcttg cagcaagttg gaagaagaaa gaagaaaaac
     9121 aaatagttaa atagtgctca cccttcgtga aaagggtggt actatttgct aaaaatagtt
     9181 ttttatagtg aggaatcata gttaggcgtg cattttcttt gcttattgat actggaatag
     9241 tga
//
LOCUS       DAZFLD010000185         9175 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.186, whole genome shotgun sequence.
ACCESSION   DAZFLD010000185 DAZFLD010000000
VERSION     DAZFLD010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9175)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9175)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9175
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.186"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            53..148
                     /gene="ymiC"
                     /locus_tag="ACHKBL_003877"
     CDS             53..148
                     /gene="ymiC"
                     /locus_tag="ACHKBL_003877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YmiC"
                     /protein_id="HFO5820679.1"
                     /translation="MINTNMKYWSWMGAFSLSMLFWAELLWIITH"
     gene            298..2973
                     /gene="acnA"
                     /locus_tag="ACHKBL_003878"
     CDS             298..2973
                     /gene="acnA"
                     /locus_tag="ACHKBL_003878"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HFO5820680.1"
                     /translation="MSSTLREASKDTLQAKDKTYHYYSLPLAAKSLGNITRLPKSLKV
                     LLENLLRWQDGNSVTEEDIHALAGWLKNAHADREIAYRPARVLMQDFTGVPAVVDLAA
                     MREAVKRLGGDTAKVNPLSPVDLVIDHSVTVDRFGDDEAFEENVRLEMERNHERYVFL
                     KWGKQAFSRFSVVPPGTGICHQVNLEYLGKAVWSELQDGEWIAYPDTLVGTDSHTTMI
                     NGLGVLGWGVGGIEAEAAMLGQPVSMLIPDVVGFKLTGKLREGITATDLVLTVTQMLR
                     KHGVVGKFVEFYGDGLDSLPLADRATIANMSPEYGATCGFFPIDAVTLDYMRLSGRSE
                     DQVELVEKYAKAQGMWRNPGDEPIFTSTLELDMNDVEASLAGPKRPQDRVALPDVPKA
                     FAASNELEVNATHKDRQPVDYVMNGHQYQLPDGAVVIAAITSCTNTSNPSVLMAAGLL
                     AKKAVTLGLKRQPWVKASLAPGSKVVSDYLAKAKLTPYLDELGFNLVGYGCTTCIGNS
                     GPLPDPIETAIKKGDLTVGAVLSGNRNFEGRIHPLVKTNWLASPPLVVAYALAGNMNI
                     NLASEPIGHDRKGDPVYLKDIWPSAQEIARAVEQVSTEMFRKEYAEVFEGTAEWKEIN
                     VTRSDTYGWQEDSTYIRLSPFFDEMQATPAPVEDIHGARILAMLGDSVTTDHISPAGS
                     IKPDSPAGRYLQGRGVERKDFNSYGSRRGNHEVMMRGTFANIRIRNEMVPGVEGGMTR
                     HLPDSDDVSIYDAAMRYKQEQTPLAVIAGKEYGSGSSRDWAAKGPRLLGIRVVIAESF
                     ERIHRSNLIGMGILPLEFPQGVTRKTLGLTGEEKIDIGDLQNLQPGATVPVTLTRADG
                     SQEVVPCRCRIDTATELTYYQNDGILHYVIRNMLK"
     gene            complement(3037..3627)
                     /gene="ribA"
                     /locus_tag="ACHKBL_003879"
     CDS             complement(3037..3627)
                     /gene="ribA"
                     /locus_tag="ACHKBL_003879"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HFO5820681.1"
                     /translation="MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHVALVYGDISGHT
                     PVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKI
                     RAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTE
                     AGINIVERVPLIVGRNPNNEHYLDTKAEKMGHLLNK"
     gene            3797..4561
                     /gene="pgpB"
                     /locus_tag="ACHKBL_003880"
     CDS             3797..4561
                     /gene="pgpB"
                     /locus_tag="ACHKBL_003880"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase B"
                     /protein_id="HFO5820682.1"
                     /translation="MRSIARRTAVGAALLLVMPVAVWISGWRWQPGEQSWLLKAAFWV
                     TETVTQPWGVITHLILFGWFLWCLRFRIKAAFVLFAILAAAILVGQGVKSWIKDKVQE
                     PRPFVIWLEKTHHIPVDEFYTLKRAERGNLVKEQLAEEKNIPQYLRSHWQKETGFAFP
                     SGHTMFAASWALLAVGLLWPRRRTLTIAILLVWATGVMGSRLLLGMHWPRDLVVATLI
                     SWALVAVATWLAQRICGPLTPPAEENREIAQREQES"
     gene            4710..5018
                     /gene="lapA"
                     /locus_tag="ACHKBL_003881"
     CDS             4710..5018
                     /gene="lapA"
                     /locus_tag="ACHKBL_003881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA"
                     /protein_id="HFO5820683.1"
                     /translation="MKYLLIFLLVLAIFVISVTLGAQNDQQVTFNYLLAQGEYRISTL
                     LAVLFAAGFAIGWLICGLFWLRVRVSLARAERKIKRLENQLSPATDVAVAPHSSAAKE
                     "
     gene            5025..6194
                     /gene="lapB"
                     /locus_tag="ACHKBL_003882"
     CDS             5025..6194
                     /gene="lapB"
                     /locus_tag="ACHKBL_003882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0008653 - lipopolysaccharide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HFO5820684.1"
                     /translation="MLELLFLLLPVAAAYGWYMGRRSAQQNKQDEANRLSRDYVAGVN
                     FLLSNQQDKAVDLFLDMLKEDTGTVEAHLTLGNLFRSRGEVDRAIRIHQTLMESASLT
                     YEQRLLAIQQLGRDYMAAGLYDRAEDMFNQLTDETDFRIGALQQLLQIYQATSEWQKA
                     IDVAERLVKLGKDKQRVEIAHFYCELALQHMASDDLDRAMALLKKGAAADKNSARVSI
                     MMGRVFMAKGEYAKAVESLQRVISQDRELVSETLEMLQTCYQQLGKTAEWAEFLQRAV
                     EENTGADAELMLADIIEARDGSEAAQVYITRQLQRHPTMRVFHKLMDYHLNEAEEGRA
                     KESLMVLRDMVGEKVRSKPRYRCQKCGFTAYTLYWHCPSCRAWSTIKPIRGLDGL"
     gene            6385..7122
                     /gene="pyrF"
                     /locus_tag="ACHKBL_003883"
     CDS             6385..7122
                     /gene="pyrF"
                     /locus_tag="ACHKBL_003883"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HFO5820685.1"
                     /translation="MTLTASSSSRAVTNSPVVVALDYHNRDAALAFVDKIDPRDCRLK
                     VGKEMFTLFGPQFVRELQQRGFDIFLDLKFHDIPNTAAHAVAAAADLGVWMVNVHASG
                     GARMMTAAREALVPFGKDAPLLIAVTVLTSMEASDLADLGVTLSPADYAERLAALTQK
                     CGLDGVVCSAQEAARFKQVFGQEFKLVTPGIRPQGSEAGDQRRIMTPEQALAAGVDYM
                     VIGRPVTQSVDPAQTLKAINASLQRSA"
     gene            7122..7448
                     /gene="yciH"
                     /locus_tag="ACHKBL_003884"
     CDS             7122..7448
                     /gene="yciH"
                     /locus_tag="ACHKBL_003884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HFO5820686.1"
                     /translation="MSDSNSRLVYSTETGRIDEPKAAPVRPKGDGVVRIQRQTSGRKG
                     KGVCLITGVDLDDAELTKLAAELKKKCGCGGAVKDGIIEIQGDKRDLLKSLLEAKGMK
                     VKLAGG"
     gene            complement(7574..7792)
                     /gene="osmB"
                     /locus_tag="ACHKBL_003885"
     CDS             complement(7574..7792)
                     /gene="osmB"
                     /locus_tag="ACHKBL_003885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmB"
                     /protein_id="HFO5820687.1"
                     /translation="MFVTSKKMTAAVLAITLAMSLSACSNWSKRDRNTAIGAGAGALG
                     GAVLTDGSTLGTLGGAAVGGVIGHQVGK"
     gene            complement(8061..8810)
                     /gene="yciT"
                     /locus_tag="ACHKBL_003886"
     CDS             complement(8061..8810)
                     /gene="yciT"
                     /locus_tag="ACHKBL_003886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YciT"
                     /protein_id="HFO5820688.1"
                     /translation="MNSRQQTILQMVIDQGQVSVTDLAKATGVSEVTIRQDLNTLEKL
                     SYLRRAHGFAVSLDSDDVETRMMSNYTLKRELAEFAASLVQPGETIFIENGSSNALLA
                     RTLGEQKKNVTIITVSSYIAHLLKDAPCEIILLGGVYQKKSESMVGPLTRQCIQQVHF
                     SKAFIGIDGWQPETGFTGRDMMRTDVVNAVLEKECEAIVLTDSSKFGAVHSYSIGPVE
                     RFNRVITDSKIRASDLMHLEHSKLTVHVVDI"
     gene            complement(8900..9073)
                     /gene="yciZ"
                     /locus_tag="ACHKBL_003887"
     CDS             complement(8900..9073)
                     /gene="yciZ"
                     /locus_tag="ACHKBL_003887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YciZ"
                     /protein_id="HFO5820689.1"
                     /translation="MSEFDAQRVAERIDIVLDILVAGDYHSAIHNLEILKAELLRQVA
                     ESTPDIPKAPWEI"
     gene            9111..>9175
                     /gene="ymiD"
                     /locus_tag="ACHKBL_003888"
     CDS             9111..>9175
                     /gene="ymiD"
                     /locus_tag="ACHKBL_003888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmiD"
                     /protein_id="HFO5820690.1"
                     /translation="MQKLPLKEKCLTATANYHPGIR"
BASE COUNT         2277 a         2165 c         2420 g         2313 t
ORIGIN      
        1 taacaggttg agttatctca acacaaagta ataaccgtta aaggtgtagc ctatgatcaa
       61 cacaaatatg aaatattggt cctggatggg cgcattttct ctgtcgatgc tcttctgggc
      121 cgaactcctc tggatcatta ctcactgatc cctgaccccg ctgtttgctt tgtctcaagg
      181 tttctcctct tttatcaatt tgggttgtta tcaaatcgtt acgcgatgtt tgtgttatct
      241 ttaatattca ccctgaagag aatcagggct tcgcaaccct gtcattaagg aggagctatg
      301 tcgtcaaccc tacgagaagc cagtaaggac acgttgcagg ccaaagataa aacttaccac
      361 tactacagcc tgccgcttgc tgccaaatca ctgggcaata tcacccgtct gcctaagtca
      421 ctcaaagttt tgctcgaaaa cctgctgcgc tggcaggatg gtaactcggt taccgaagag
      481 gatatccacg cgctggcggg atggctgaaa aatgcccatg ctgaccgtga aattgcctac
      541 cgcccggcaa gggtgctgat gcaggacttt accggcgtac ctgccgttgt tgatctggcg
      601 gcaatgcgcg aagcggttaa acgcctcggt ggcgatactg caaaggttaa cccgctctca
      661 ccggtcgacc tggtcattga ccactcggtg accgtcgatc gttttggtga tgatgaggca
      721 tttgaagaaa acgtacgcct ggaaatggag cgcaaccacg aacgttatgt gttcctgaaa
      781 tggggaaagc aggcgttcag tcgttttagc gtcgtgccgc caggcacagg catttgccat
      841 caggttaacc tcgaatatct cggcaaagca gtgtggagcg aattgcagga cggtgaatgg
      901 attgcttatc cggatacact cgttggtact gactcgcaca ccaccatgat caacggcctt
      961 ggcgtgctgg ggtggggcgt tggtggcatc gaagccgaag ccgcaatgtt aggccagccg
     1021 gtttccatgc ttatcccgga cgtagtgggc tttaaactca ccggaaaatt acgtgaaggt
     1081 attaccgcca cagacctggt tctcaccgtt acccaaatgc tgcgcaaaca tggcgtggtg
     1141 gggaaattcg tcgaatttta tggtgatggg ctggattcac taccgctggc ggatcgcgcc
     1201 accattgcca atatgtcgcc agaatatggt gccacctgtg gcttcttccc aatcgatgct
     1261 gtcaccctcg attacatgcg tttaagcggg cgcagcgaag atcaggtcga gttggtcgaa
     1321 aaatatgcca aagcgcaggg catgtggcgt aacccgggcg atgaaccaat ttttaccagt
     1381 acgttagaac tggatatgaa tgacgttgaa gcgagcctgg cagggcctaa acgcccacag
     1441 gatcgcgttg cactgcccga tgtaccaaaa gcatttgccg ccagtaacga actggaagtg
     1501 aatgccacgc ataaagatcg ccagccggtc gattatgtta tgaacggaca tcagtatcag
     1561 ttacctgatg gcgcggtggt cattgctgcg ataacctcgt gcaccaacac ctctaaccca
     1621 agtgtgctga tggccgcagg cttgctggcg aaaaaagctg taactctggg cctcaagcgg
     1681 caaccgtggg tcaaagcgtc gctggcacca ggctcgaaag ttgtttctga ctatctggca
     1741 aaagcgaaat tgacaccgta tctcgacgaa ctggggttta accttgtggg gtacggttgt
     1801 accacctgta ttggtaactc tgggccgctg cccgatccta tcgaaacggc aatcaaaaaa
     1861 ggcgatttaa ccgtcggtgc ggtgctgtcc ggcaaccgta actttgaagg ccgtatccat
     1921 ccgctggtta aaactaactg gctggcctcg ccgccgctgg tggttgccta tgcgctggcg
     1981 ggaaatatga atattaacct cgcttctgag cctattggtc acgatcgtaa aggcgacccg
     2041 gtatatctga aagatatctg gccctcggca caagaaattg cccgtgcggt agaacaagtc
     2101 tccacagaaa tgttccgcaa agagtacgca gaagtttttg aaggcacagc agagtggaag
     2161 gaaattaacg tcacacgatc cgatacctac ggttggcagg aagattcaac ctatattcgc
     2221 ttatcgccat tctttgatga aatgcaggca acaccagcac ctgtggaaga tattcacggt
     2281 gcgcggatcc tcgcaatgct gggggattca gtcaccactg accatatctc tccggcgggc
     2341 agtattaagc ccgacagccc ggcgggtcga tatctacaag gtcggggtgt tgagcgaaaa
     2401 gactttaact cctacggttc gcggcgtggt aaccatgaag tgatgatgcg cggcaccttc
     2461 gccaatattc gcatccgtaa tgaaatggtg cctggcgttg aaggggggat gacgcggcat
     2521 ttacctgaca gcgatgatgt ctctatctat gatgccgcga tgcgctataa gcaggagcaa
     2581 acgccgctgg cggtgattgc cgggaaagag tatggatcag gctccagccg tgactgggcg
     2641 gcaaaaggtc cgcgtctgct tggtattcgt gtggtgattg ccgaatcgtt tgaacgaatt
     2701 caccgttcga atttaattgg catgggcatc ctgccgctgg aatttccgca aggcgtaacg
     2761 cgtaaaacgt tagggctaac cggggaagag aagattgata ttggcgatct gcaaaaccta
     2821 caacccggcg cgacggttcc ggtgacgctt acgcgcgcgg atggtagcca ggaagtcgta
     2881 ccctgccgtt gtcgtatcga caccgcgacg gagttgacct actaccagaa cgacggcatt
     2941 ttgcattatg tcattcgtaa tatgttgaag taacaagtat ttgcttgccg gttattttgc
     3001 ttccggcaag caaatgaatt acacaatgca agagggttat ttgttcagca aatggcccat
     3061 tttctcggct ttggtatcga gataatgttc gttattgggg ttacgaccta caatcaatgg
     3121 tacgcgttca acaatattaa tccctgcttc ggtcagaatt tcgacttttt tcgggttatt
     3181 ggttaacaag cggacttcat tgacgccaag gagtttgaac atatcagcgc aaagagtgaa
     3241 gtcgcgctca tcagcggcga agcctaactg gtggttagcc tctacagtat cgtaaccttg
     3301 atcctgcagt gcataagcgc ggattttatt cagcagacca atgttacgac cttcctgacg
     3361 gtgatacagc aaaataccac ggccttcttc ggcaatttgc gtcagtgccg cttcgagctg
     3421 gaagccacaa tcgcagcgca agctgaacag ggcgtcaccg gtcagacatt cggaatggac
     3481 gcgcgcaagt accggggtat gcccggagat atcgccatag actagcgcga catgatcgtg
     3541 tccggttgcc agttcttcaa atcccaccat caggaaatcg ccccatgggg ttggcagttt
     3601 ggcttctgcc acacgtttaa gctgcatgaa attctccaga taatgctggt tctgtattgg
     3661 cttattttgc cataacgaga aggggttcac ctaatcacga cgcgtcgatc gttcacggaa
     3721 tggcacaaat ctgtcaattt ttcctggaac tggcgttttc agttatgatt gtgggactta
     3781 tcaaaaagga gaggccatgc gttcgattgc cagacgtacc gcagtgggag ctgcactatt
     3841 gcttgtcatg ccagtagccg tatggatttc tggttggcgt tggcaacctg gagaacaaag
     3901 ttggctacta aaagcggctt tttgggttac tgaaactgtc acccagccct ggggcgtcat
     3961 tacacatttg attttattcg gctggtttct ctggtgtctg cgttttcgca ttaaggctgc
     4021 ctttgtatta tttgccattc tggcggcggc aattcttgtg ggacaaggcg ttaaatcctg
     4081 gatcaaagac aaagtccagg aaccacgacc ttttgttatc tggctggaaa aaacacatca
     4141 tattccggtt gatgagttct acactttaaa gcgagcagaa cgcggaaatc tggtgaaaga
     4201 acagttggct gaagagaaaa atatcccaca atatttgcgt tcacactggc agaaagagac
     4261 ggggtttgcc tttccttccg gtcacacgat gtttgctgcc agttgggcgc tgctggccgt
     4321 tggtttgctg tggccgcgtc ggcgaacgtt aaccattgct atcttgttgg tctgggcaac
     4381 gggagtcatg ggaagccgcc tgctgctcgg gatgcattgg ccacgcgatc tggtggtagc
     4441 tacgttgatt tcgtgggcgc tggtggcggt ggcaacgtgg cttgcgcaac gaatttgtgg
     4501 gccattaaca ccacctgcgg aagaaaatcg cgaaatagcg caacgagaac aagaaagtta
     4561 acgctggttg attttccgaa tttagccctt aaatcatcat caatgcgcgt ggatgccatt
     4621 tcgcagacgg cgcgaaaatg gtactttaaa gggctattgc ggtaagttga ccataattta
     4681 ttcgctctaa ccacataacg ggaagtaatg tgaaatattt actcattttc ttactggtgt
     4741 tagcgatctt cgtgatttcg gtcacgttag gtgcgcagaa cgatcaacag gtgacgttta
     4801 attatctgtt agcgcaaggg gagtaccgta tttccacatt gctggcggta ttgtttgctg
     4861 cggggtttgc tatcggttgg ctgatttgcg gtctgttctg gctgcgagtt cgtgtttccc
     4921 tggcgcgcgc tgaacgtaaa ataaagcgac tggaaaacca gctttcaccc gcgactgacg
     4981 tggctgtagc gccgcattcg tcagcggcga aggaataact ttctatgctg gagttgttgt
     5041 ttctgctttt gcctgtagcc gctgcctatg gctggtatat gggccgcaga agtgcgcaac
     5101 aaaacaagca agatgaagcc aaccgcttgt cgcgtgatta cgtagcgggg gttaacttcc
     5161 tgcttagtaa tcaacaggat aaagcggtag acctgtttct cgatatgctt aaagaggata
     5221 cgggtaccgt tgaagcccac cttacgctag gaaacctgtt ccgttcgcgt ggcgaagttg
     5281 atcgcgctat tcgcatccat cagaccctaa tggaaagcgc ctcgctgacc tatgaacagc
     5341 gtctgttggc gattcaacaa ctggggcgtg attacatggc cgccgggtta tacgaccgcg
     5401 cggaagacat gttcaatcag ctgaccgatg aaactgactt ccgcattggc gcgctgcaac
     5461 agttgctaca aatctaccag gctaccagcg agtggcagaa agcaattgat gttgccgaac
     5521 gtctggtgaa gttgggtaaa gataagcagc gcgtcgaaat tgcccatttc tactgtgagt
     5581 tagccctgca gcatatggcc agcgacgatc tcgatcgcgc catggccttg ttaaaaaaag
     5641 gtgcggcggc agataaaaac agcgcccgcg tatccatcat gatgggacgc gtatttatgg
     5701 cgaaaggaga atacgccaaa gccgtcgaaa gtctgcaacg cgtgatatct caggacagag
     5761 aactggtcag cgaaacgctg gaaatgttgc aaacctgcta tcagcagttg ggtaaaactg
     5821 ccgaatgggc agaattcctg cagcgcgcgg tggaagagaa caccggtgcc gatgctgaat
     5881 tgatgcttgc ggatattatc gaagcgcgcg acggtagtga ggccgcacag gtctatatta
     5941 cgcgtcagct tcagcgtcat ccgaccatgc gtgtgttcca taagctaatg gattaccact
     6001 taaatgaagc ggaagaaggg cgtgccaaag agagtctgat ggtgctgcgt gacatggttg
     6061 gcgagaaggt gcgtagtaag cctcgttatc gctgccagaa atgtggtttt accgcataca
     6121 ccctctactg gcattgtccg tcttgtcggg cctggtcaac cattaaaccg attcgcggtc
     6181 ttgatggcct gtaattttta aaaaaatccg actttagtta caacatacta ataattgatg
     6241 ttcattgtgc tccggtaact ccggcgcaga aaagcctgcc aggggagaaa tcgcaactgt
     6301 taatttttta tttccacggg tagaatgctc gccgtttacc tgtttcgcgc cacttccggt
     6361 gcccatcatc aagaaggtct ggtcatgacg ttaactgctt catcttcttc ccgcgctgtt
     6421 acgaattctc ctgtggttgt tgcccttgat tatcataatc gtgatgccgc gctggccttt
     6481 gtcgacaaga tcgacccacg cgattgtcgt ctgaaagtcg gcaaagagat gtttacgctg
     6541 tttggacctc aatttgtacg cgaacttcaa cagcgtggtt ttgatatctt cctcgacctg
     6601 aaatttcacg atattcccaa cactgcagcg cacgctgtcg ctgctgcagc tgacttaggc
     6661 gtgtggatgg tgaatgttca cgcctccggt ggggcgcgta tgatgaccgc agcgcgtgag
     6721 gcactggtgc cgtttggcaa agatgcaccg cttttgattg ctgtgacagt attgaccagc
     6781 atggaagcca gcgacctggc cgatcttggc gtgacactgt cacctgcaga ttatgcagaa
     6841 cgtctggcgg cactgacgca aaaatgtggc cttgatggtg tggtgtgttc tgctcaggaa
     6901 gctgcgcgct ttaaacaggt atttggtcag gagttcaaac tggttacgcc gggcattcgt
     6961 ccgcagggga gtgaagctgg tgaccagcgc cgcattatga cgccagaaca ggcgttggcg
     7021 gctggtgttg attatatggt gattggtcgc ccggtaacgc aatcggtaga tccagcgcaa
     7081 acgctgaaag cgatcaacgc ctctttacag cggagtgcat gatgagtgat tccaacagcc
     7141 gtctggtcta ctcaacggaa accggacgta ttgatgagcc aaaagcggcc cctgtacgcc
     7201 ctaaaggcga cggtgtggtg cgtattcagc gtcagaccag tggacgtaaa ggtaagggcg
     7261 tttgcctgat taccggtgtc gatctcgatg atgccgaact gacaaaactg gcagcggaac
     7321 tgaagaaaaa atgcggctgc ggcggggcag ttaaagatgg aattattgaa atccagggcg
     7381 ataagcgtga tttattaaaa tcactgcttg aagcgaaagg gatgaaggta aaactcgccg
     7441 gtggttaaca taaaaagcca cggatatatc cgtggctttc gaatatttta ctgtgtgtat
     7501 tattcagtta tttcgtactg aacagaatca agcgtattat aattgcgagt cgataatcga
     7561 catttattta cgattattta ccgacctggt gaccaataac accaccgaca gctgcgccac
     7621 ctaatgtacc caacgtactg ccatcggtca gtactgcacc gcctaatgcc cctgcacccg
     7681 cgccgattgc ggtgttgcga tcccgtttag accagttaga acaggcactc agagacattg
     7741 ccagggtaat tgccagaaca gccgcggtca tttttttgct cgttacaaac ataatactct
     7801 ctcctgaatt tatgattcac ggaagtaagc tctctataac tataatagct aagaataagc
     7861 ctggtgaaat tattccgtga aatccgctcg cgcgcagggt tatatcacgc aggtgataat
     7921 gacttcctgt tatatcgctg ataataattt tatatcttga gagtgttaat aacaggtaaa
     7981 tagtcttaat tatcaaccag gaatcatctt agagcggatg atttgccaaa gtgcaaatca
     8041 tccgtagaga agggaaatgg ttaaatgtca acaacgtgaa cggtgagttt actgtgctca
     8101 agatgcatca gatcgctggc gcgtattttc gaatcggtaa tcacgcgatt gaatcgctca
     8161 acgggaccga tggagtatga atgtacagca ccaaatttcg agctgtcagt caggactatc
     8221 gcttcgcact ctttttccag cacggcattg accacatcgg tacgcatcat atcgcgaccg
     8281 gtgaatccag tttcaggttg ccagccatca ataccaataa atgctttgct gaaatgtacc
     8341 tgttggatac actggcgtgt caacgggcca accatacttt cgcttttttt ctggtacacg
     8401 ccaccgagca aaataatttc acaaggcgcg tctttcagca aatgcgcgat gtagctgctg
     8461 accgtgatga tagtgacatt tttcttctgc tcgcccagag tccgggcgag cagggcattg
     8521 ctgctgccat tttcgataaa gatggtttcg cccggttgaa ccagtgacgc ggcaaactcg
     8581 gcgagttcac gcttcagcgt atagttgctc atcatacggg tttcgacgtc atcactatca
     8641 agcgatactg caaagccatg tgcacggcgg aggtaactca gtttttcgag ggtgttgaga
     8701 tcctggcgaa tggtaacttc agaaactcca gtggcttttg ccagatcggt tacgctaacc
     8761 tgaccctggt caatgaccat ctgtagaata gtttgttgtc gggaattcat cgctgtaatt
     8821 cttataacgt tataatacta aataaaaaaa tactgacgcg ccgggcatcc tcaaatagga
     8881 aaacctgaag gcacgattgt taaatctccc acggtgcctt cgggatgtct ggcgtagatt
     8941 cagcaacctg gcgcaagagt tctgctttaa gtatctcgag attatggatc gcagagtggt
     9001 aatcgcctgc cacaaggata tctaacacaa tatcaatacg ttcggcaacg cgctgggcat
     9061 caaattcaga catcaaggac atccttatag caaaagtggg aaaatgaata atgcaaaaat
     9121 tgccgctaaa agagaagtgt ttaacagcaa cggctaatta tcatccggga atacg
//
LOCUS       DAZFLD010000186         9139 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.187, whole genome shotgun sequence.
ACCESSION   DAZFLD010000186 DAZFLD010000000
VERSION     DAZFLD010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9139)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9139)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9139
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.187"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            103..465
                     /locus_tag="ACHKBL_003889"
     CDS             103..465
                     /locus_tag="ACHKBL_003889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5820691.1"
                     /translation="MNEFEKIFNEMNLDRALLPILFRSNRSTVWKYLSGDSTAPASAM
                     SLIMLLQLIQKRNPDLLAEWLTLSDFTIPPEVYLDQPDYWKGWVYTQHKVNKNVLEYL
                     KKHYPDEDQKSMGKGREE"
     gene            complement(568..1026)
                     /gene="ybbJ"
                     /locus_tag="ACHKBL_003890"
     CDS             complement(568..1026)
                     /gene="ybbJ"
                     /locus_tag="ACHKBL_003890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HFO5820692.1"
                     /translation="MMELMVVHPHIFWLSLGGLLLAAEMLGGNGYLLWSGVAAVITGL
                     VVWLLPLGWEWQGVMFAVLTLLAAWLWWKWLSRRVREQKHSDSHLNQRGQQLIGRRFV
                     LESPLVNGRGHMRVGDSSWPVSASEDLSAGTHVEVIAIEGITLIIRAVIA"
     gene            complement(1023..1940)
                     /gene="qmcA"
                     /locus_tag="ACHKBL_003891"
     CDS             complement(1023..1940)
                     /gene="qmcA"
                     /locus_tag="ACHKBL_003891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFO5820693.1"
                     /translation="MLIFIPILIFVALVIVGAGVKIVPQGYQWTVERFGRYTKTLQPG
                     LSLVVPFMDRIGRKINMMEQVLDIPSQEVISKDNANVTIDAVCFIQVIDAPRAAYEVS
                     NLELAIINLTMTNIRTVLGSMELDEMLSQRDSINSRLLRIVDEATNPWGIKVTRIEIR
                     DVRPPAELISSMNAQMKAERTKRAYILEAEGIRQAEILKAEGEKQSQILKAEGERQSA
                     FLQAEARERSAEAEARATKMVSEAIASGDIQAVNYFVAQKYTEALQQIGSSSNSKVVM
                     MPLEASSLMGSIAGIAELVKDSANKRTQP"
     gene            2086..2763
                     /gene="fetA"
                     /locus_tag="ACHKBL_003892"
     CDS             2086..2763
                     /gene="fetA"
                     /locus_tag="ACHKBL_003892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein FetA"
                     /protein_id="HFO5820694.1"
                     /translation="MQENSPLLQLQNVGYLAGDAKILNNINFSLRAGEFKLITGPSGC
                     GKSTLLKIVASLISPTSGTLLFEGEDVSTLKPEIYRQQVSYCAQTPTLFGDTVYDNLI
                     FPWQIRNQQPDPAIFLDFLERFALPDTILTKNIAELSGGEKQRISLIRNLQFMPKVLL
                     LDEITSALDESNKHNVNEMIHRYVREQNIAVLWVTHDKDEINHADKVITLQPHAGEMQ
                     EARYELA"
     gene            2750..3529
                     /gene="fetB"
                     /locus_tag="ACHKBL_003893"
     CDS             2750..3529
                     /gene="fetB"
                     /locus_tag="ACHKBL_003893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HFO5820695.1"
                     /translation="MNSHNITNESLALALMLVVVAILISHKEKLALEKDILWSVGRAI
                     IQLIIVGYVLKYIFSVDDASLTLLMVLFICFNAAWNAQKRSKYIAKAFISSFIAITVG
                     AGITLAVLILSGSIEFIPMQVIPIAGMIAGNAMVAVGLCYNNLGQRVISEQQQIQEKL
                     SLGATPKQASAILIRDSIRAALIPTVDSAKTVGLVSLPGMMSGLIFAGIDPVKAIKYQ
                     IMVTFMLLSTASLSTIIACYLTYRKFYNSRHQLVVTQLKKK"
     gene            complement(3592..4446)
                     /gene="cnoX"
                     /locus_tag="ACHKBL_003894"
     CDS             complement(3592..4446)
                     /gene="cnoX"
                     /locus_tag="ACHKBL_003894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperedoxin"
                     /protein_id="HFO5820696.1"
                     /translation="MSVENIVNINESNLQQVLEQSMTTPVLFYFWSERSQHCLQLTPI
                     LESLAAQYNGQFILAKLDCDAEQMIAAQFGLRAIPTVYLFQNGQPVDGFQGPQPEEAI
                     RALLDKVLPREEELKAQQAMQLMQEGNYTDALPLLKDAWQLSNQNGEIGLLLAETLIA
                     LNRSEDAEAVLKTIPLQDQDTRYQGLVAQIELLKQAADTPEIQQLQQQVAENPEDAAL
                     ATQLALQLHQVGRNEEALELLFGHLRKDLTAADGQTRKTFQEILAALGTGDALASKYR
                     RQLYALLY"
     gene            complement(4507..5316)
                     /gene="ybbO"
                     /locus_tag="ACHKBL_003895"
     CDS             complement(4507..5316)
                     /gene="ybbO"
                     /locus_tag="ACHKBL_003895"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HFO5820697.1"
                     /translation="MTHKATEILTGKVMQKSVLITGCSSGIGLESALELKRQGFHVLA
                     GCRKPDDVERMNSMGFTGVLIDLDSPESVDRAADEVIALTDNCLYGIFNNAGFGMYGP
                     LSTISRAQMEQQFSANFFGAHQLTMRLLPAMLPHGEGRIVMTSSVMGLISTPGRGAYA
                     ASKYALEAWSDALRMELRHCGIKVSLIEPGPIRTRFTDNVNQTQSDKPVENPGIAARF
                     TLGPEAVVDKVRHAFISEKPKMRYPVTLVTWAVMVLKRLLPGRVMDKILQG"
     gene            complement(5306..5929)
                     /gene="tesA"
                     /locus_tag="ACHKBL_003896"
     CDS             complement(5306..5929)
                     /gene="tesA"
                     /locus_tag="ACHKBL_003896"
                     /EC_number="3.1.1.5"
                     /EC_number="3.1.2.14"
                     /EC_number="3.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016298 - lipase activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional acyl-CoA thioesterase I/protease
                     I/lysophospholipase L1"
                     /protein_id="HFO5820698.1"
                     /translation="MNFNNVFRWHLPFLFLVLLTFRAAAADTLLILGDSLSAGYRMSA
                     SAAWPALLNDKWQSKTSVVNASISGDTSQQGLARLPALLKQHQPRWVLVELGGNDGLR
                     GFQPQQTEQTLRQILQDVKAANAEPLLMQIRLPANYGRRYNEAFSAIYPKLAKEFDVP
                     LLPFFMEEVYLKPQWMQDDGIHPNRDAQPFIADWMAKQLQPLVNHDS"
     gene            5900..6586
                     /gene="ybbA"
                     /locus_tag="ACHKBL_003897"
     CDS             5900..6586
                     /gene="ybbA"
                     /locus_tag="ACHKBL_003897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter ATP-binding protein
                     YbbA"
                     /protein_id="HFO5820699.1"
                     /translation="MPAENIVEVHHLKKSVGQGEHELSILTGVELVVKRGETIALVGE
                     SGSGKSTLLAILAGLDDGSSGEVSLVGQPLHNMDEEARAKLRAKHVGFVFQSFMLIPT
                     LNALENVELPALLRGESSAESRNGAKALLEQLGLGKRLDHLPAQLSGGEQQRVALARA
                     FNGRPDVLFADEPTGNLDRQTGDKIADLLFSLNREHGTTLIMVTHDLQLAARCDRCLR
                     LVNGQLQEEA"
     gene            6583..8997
                     /gene="ybbP"
                     /locus_tag="ACHKBL_003898"
     CDS             6583..8997
                     /gene="ybbP"
                     /locus_tag="ACHKBL_003898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter permease subunit YbbP"
                     /protein_id="HFO5820700.1"
                     /translation="MIARWFWREWRSPSLLIVWLALSLAVACVLALGNISDRMEKGLS
                     QQSREFMAGDRALRSSREVPQAWLEEAQKRGLKVGKQLTFATMTFAGDTPQLANVKAV
                     DDIYPMYGDLQTNPPGLKPQAGSVLLAPRLMALLNLKTGDTIDVGDATLRIAGEVIQE
                     PDSGFNPFQMAPRLMMNLADVDKTGAVQPGSRVTWRYKFGGSENQLDGYEKWLLPQLK
                     PEQRWYGLEQDEGALGRSIERSQQFLLLSALLTLLLAVAAVAVAMNHYCRSRYDLVAI
                     LKTLGAGRAQLRKLIVGQWLMVLALSAVTGGAIGLLFENVLMVLLKPVLPAALPPASL
                     WPWLWALGTMTVISLLVGLRPYRLLLATQPLRVLRNDVVANVWPLKFYLPIVSVVVVL
                     LLAGLMGGSMLLWAVLAGAVVLALLCGVLGWMLLNVLRRMTLKSLPLRLAVSRLLRQP
                     WSTLSQLSAFSLSFMLLALLLVLRGDLLDRWQQQLPPESPNYFLINIATEQVAPLKAF
                     LAEHQIVPESFYPVVRARLTAINDKPTEGNEDEALNRELNLTWQNTRPDHNPIVAGNW
                     PPKADEVSMEEGLAKRLNVALGDTVTFMGDTQEFRAKVTSLRKVDWESLRPNFYFIFP
                     EGALDGQPQSWLTSFRWENGNGMLTQLNRQFPTISLLDIGAILKQVGQVLEQVSRALE
                     VMVVLVTACGMLLLLAQVQVGMRQRHQELVVWRTLGAGKKLLRTTLWCEFAMLGFVSG
                     LVAAIGAETALAVLQAKVFDFPWEPDWRLWIVLPCSGALLLSLFGGWLGARLVKGKAL
                     FRQFAG"
BASE COUNT         2075 a         2339 c         2513 g         2212 t
ORIGIN      
        1 tgtttctgga ctaaacgtga agcggttcct gatgtgtgct aaaatgcgtt tctttgataa
       61 atctcaattt ttaagaggtt tacgccaggc aggaaaattt aaatgaatga atttgagaag
      121 atttttaatg aaatgaatct ggaccgggca ttgttaccga tcctttttcg ttcgaaccgg
      181 tcgacagtgt ggaagtattt aagcggcgat agtacggctc ctgcctctgc catgtcgctg
      241 atcatgctgt tgcagttgat ccagaaacga aaccccgatc tgttagccga atggttaacg
      301 ctttctgatt tcaccattcc gccagaagtt tacctggacc aacctgatta ctggaaaggg
      361 tgggtgtata cccaacacaa ggtcaataaa aacgtcctgg aatatctaaa aaaacactat
      421 ccggatgaag accaaaaaag tatgggtaaa ggccgggaag agtaattttg cctctactcg
      481 gctatatgcc ggatgcggcg caagcgcctt atctggccta cgattcgggc atattttgta
      541 ggcatgataa gacgcgccag cgtcgcatca ggcgatgacc gcacggataa tcagcgttat
      601 cccttctatc gcaatcacct caacatgcgt acctgcactg agatcctcgc tggcgctgac
      661 aggccatgaa ctgtcaccga cgcgcatatg accacgcccg ttgaccagcg gagattccag
      721 aacaaaacgt cggccaatca actgctgccc gcgctggttt aaatgactgt cgctgtgctt
      781 ttgttcgcgc acccgccgcg acaaccattt ccaccacagc caggcggcga gcagcgtcag
      841 gacggcaaac attacccctt gccactccca acccagcggc agcaaccaga ccaccagacc
      901 ggtaatcacc gctgccacgc cgctccacaa cagataacca ttgccgccca acatctcggc
      961 tgccagcagc aaaccgccga gacttagcca gaaaatatgt ggatgaacga ccattaactc
     1021 catcatggct gagtccgttt gttggcgctg tctttcacca gctcagcaat cccggcaatc
     1081 gaccccatca ggctgctggc ctctaatggc atcatcacca ctttgctgtt actggacgaa
     1141 ccgatctgct gtaacgcttc ggtgtatttc tgtgcgacga agtagttcac cgcctgaata
     1201 tcaccggagg caatggcttc agacaccatt ttagtggcgc gggcttctgc ttcagcggaa
     1261 cgttcacgcg cttcagcctg taaaaacgcc gactgacgtt cgccttccgc tttcaggatt
     1321 tgcgactgtt tttcaccttc ggctttgagg atttccgcct gacggatccc ttccgcttca
     1381 aggatgtaag cgcgtttggt acgttccgct ttcatctgcg cgttcattga agagataagc
     1441 tctgccggtg ggcgcacgtc gcgaatttca atacgggtga ctttaatccc ccacgggttg
     1501 gtggcctcat cgacaatacg cagcaggcgt gagttgatgc tgtcgcgctg agagagcatt
     1561 tcgtcaagtt ccattgaacc cagcacggta cggatgttag tcatggtcag gttgatgatc
     1621 gccagctcca gattgctgac ttcataagcc gcgcgcggcg cgtcaatcac ctgaataaag
     1681 cacacggcgt cgatggtaac gttggcgtta tctttcgaga taacttcctg ggaagggata
     1741 tcgagcactt gctccatcat attgatcttg cgaccaatgc gatccataaa cggcaccacc
     1801 agactgagcc ccggctgtaa cgttttggta tagcgaccaa aacgttctac tgtccactga
     1861 tagccctgcg gcacgatttt gacgcccgcg ccgacaatga ccagcgcgac aaaaatgaga
     1921 atcgggataa agataagcat cggaaaaacc tcctgttgta ccgtccataa tcagcaaaat
     1981 tgctgcttga ttaaacaaat tatacctgat tactgaaaga gagttccccc ttattcctgc
     2041 gaaggataaa ctgtttttag taaaaatcag aaaaagggaa cagtgatgca ggaaaatagt
     2101 cctttgcttc agctacaaaa cgtaggatat ctggcgggtg atgcgaagat tcttaataac
     2161 atcaattttt cgctgcgtgc tggcgaattt aagttaatta ccggtccttc tggttgtggc
     2221 aaaagtacgc tgctaaaaat agttgcttca ttgatcagcc caaccagcgg aacgttactg
     2281 tttgaaggtg aggatgtcag cacactaaag ccagaaatct accgccagca agtctcttat
     2341 tgcgcccaga cgccgacgct gtttggcgac acggtatacg ataatctgat ctttccctgg
     2401 cagatccgta accagcagcc tgacccagcc atttttctcg attttctcga acgcttcgcc
     2461 ttaccggaca ccattttgac gaagaatatc gccgagctat ctggtggtga aaaacaacgc
     2521 atctcattga ttcgtaacct gcaatttatg ccgaaggttt tattgctgga tgaaataacc
     2581 agtgcgctgg acgaaagtaa taaacataac gttaatgaga tgatccaccg ttatgtgcgc
     2641 gagcaaaata ttgccgtgct gtgggtgaca cacgataaag acgaaattaa tcatgcggat
     2701 aaagtgatta cactgcaacc gcatgccgga gaaatgcagg aagcacgcta tgaactcgca
     2761 taatattact aacgaatcat tagcactggc attgatgctg gtggtggtgg caatcttaat
     2821 tagccataaa gaaaaactgg cgctggagaa agatattctc tggagcgtcg ggcgcgcgat
     2881 aatccagctg attattgtcg gctatgtgct gaagtatatt ttcagcgtgg atgatgccag
     2941 cctgacatta ttgatggtgt tatttatctg ctttaatgcg gcgtggaacg cgcaaaaacg
     3001 cagtaaatat attgctaaag cttttatctc atcgtttatt gccattacgg tcggggcggg
     3061 aattaccctg gcggtgctga ttctctcagg gtcgattgaa tttatcccga tgcaggtgat
     3121 ccctatcgcc gggatgatag ccggcaacgc catggtagcg gtggggttgt gttacaacaa
     3181 tttagggcaa cgggtcatta gcgaacagca acagattcag gagaaactga gtcttggcgc
     3241 gacgccgaaa caggcttcag cgatattgat tcgcgacagt attcgcgcgg ctttaattcc
     3301 gacggtcgat tcagcaaaaa cggtaggctt agtgagttta ccaggaatga tgtccgggct
     3361 gatatttgcc gggattgatc cggtgaaggc gattaaatat cagattatgg tgacctttat
     3421 gctgctctca accgccagct tgtcgaccat tattgcctgc tatttaacct atcgtaagtt
     3481 ttacaattcg cgccaccaat tggtggtgac gcaattgaag aagaaatgat gcgatgccgg
     3541 acgcgcagca tcgcatccgg cgttgtggtt catgtgccga tcaacggaaa ctcaatacaa
     3601 caacgcgtac agctggcggc gatacttcga cgccagtgca tcacccgtac ccagcgcagc
     3661 gaggatctcc tggaatgttt tacgtgtctg accatctgcg gcggtgagat ctttacgcag
     3721 atgcccaaac agtaactcca gcgcctcttc attgcgccca acctggtgca gttgcagtgc
     3781 cagttgtgtc gccagtgcgg catcttctgg attctccgcc acctgctgtt gcaactgttg
     3841 aatttccggc gtatcagccg cctgcttcag cagttcgatt tgcgccacca gcccctgata
     3901 gcgggtgtcc tgatcctgca acggaatggt tttcagcacg gcttctgcat cttcagaacg
     3961 gttcagcgca atcagcgttt ctgccagcag caggccgatc tccccgttct gattcgacaa
     4021 ctgccaggcg tctttcagca atggcaaggc gtcggtgtaa ttgccttcct gcatcagctg
     4081 catcgcctgc tgcgctttca gctcttcttc gcgcggcagc actttatcca gcagggcgcg
     4141 gatcgcctct tccggttgcg gcccctggaa gccatctacc ggttgcccgt tctggaacag
     4201 atacacggtc ggaatcgcgc gcagaccaaa ctgggcggca atcatctgct ccgcgtcgca
     4261 gtccagcttc gccagaataa attgcccgtt gtactgcgcc gcgaggcttt ccaggatcgg
     4321 ggttaactgc aaacagtgct ggctacgttc agaccaaaaa tagaacagca ccggagtggt
     4381 catcgactgt tcaagaacct gttgcaggtt agattcgtta atgttgacaa tattttctac
     4441 ggacatggag tcgctctctg ttgtcgatat tttctttgac atgggggctt aagcgcgcgc
     4501 ttcaactcac ccctgcaata ttttgtccat cacgcgcccc ggcagcaggc gcttaagcac
     4561 cattaccgcc caggtcacca gcgttaccgg atagcgcatc ttcggcttct cgctaataaa
     4621 agcatggcgt actttgtcca ccaccgcttc cggtcccaac gtaaagcggg cggcgatgcc
     4681 gggattttcg actggtttat cactttgcgt ctggttgacg ttgtcggtga agcgagtacg
     4741 aatgggaccg ggttcgatca ggctgacttt aattccacag tggcgtagct ccatgcgcag
     4801 cgcgtctgac cacgcctcca gcgcatattt actggccgcg taagcaccac gacccggcgt
     4861 ggagattaat cccatcaccg atgaggtcat cacaatacgc ccttcaccgt gcggtaacat
     4921 cgcgggtaac aggcgcatgg tgagctggtg tgcgccgaaa aagttggcgg aaaactgctg
     4981 ttccatctgc gcacggctga tggtggaaag ggggccatac atgccgaatc cggcattgtt
     5041 aaagatccca tacagacaat tatcggtcag ggcgatcacc tcgtcggctg cgcgatcaac
     5101 actttctggt gaatccagat cgatcaacac gccggtaaat cccatgctgt tcatgcgctc
     5161 aacatcatcc ggtttccggc aacctgccag cacatgaaaa ccctggcgtt ttaattcgag
     5221 cgcgctttcc aggccaattc cactggaaca tccggtaatt aagaccgatt tttgcataac
     5281 tttacctgtc aggatctccg ttgctttatg agtcatgatt tactaaaggc tgcaactgct
     5341 tcgccatcca gtcggcaata aacggctggg cgtcgcggtt gggatgaata ccgtcatcct
     5401 gcatccattg tggtttgagg tagacctctt ccataaaaaa gggcagcagc ggaacatcaa
     5461 actctttggc gagtttgggg taaatggcgc taaaggcttc attataacgg cgaccatagt
     5521 ttgcaggcag acgtatttgc attaacaatg gttcagcgtt ggcggctttg acatcctgca
     5581 atatctggcg cagcgtttgc tcggtttgtt gtggctgaaa accacgcaaa ccgtcattgc
     5641 cgcccagttc taccagcacc cagcgcggct gatgctgttt cagcagagcc ggaaggcgcg
     5701 ccagcccttg ttgcgaggtg tcgccgctga tgctggcatt gactaccgac gttttactct
     5761 gccacttatc gttcaacaag gcaggccagg ccgcgctggc agacattcga tacccggcgc
     5821 tcaggctatc acccagaatc aataacgtgt ccgctgcggc ggcacggaag gttaacagga
     5881 ccaggaacag gaagggcaaa tgccagcgga aaacattgtt gaagttcatc atcttaagaa
     5941 gtccgtcggt cagggggagc atgaactttc catcctcacc ggagttgagc tggttgtcaa
     6001 acgtggcgag accatcgcac tggtgggcga gtcgggatcg ggtaagtcaa ctttgctggc
     6061 gatcctcgcc gggctggatg acggcagcag tggcgaagtg agtctggtgg gacaaccgct
     6121 acataatatg gacgaagaag cgcgggcaaa gttgcgcgcg aagcacgtcg gctttgtttt
     6181 tcagtcattt atgttaattc ctacccttaa cgcgctggaa aacgtcgagc ttccggcgct
     6241 gctgcgcggt gagagtagcg cggaaagtcg taacggggcg aaagcgttgc tcgaacagtt
     6301 agggctgggt aaacgtctgg atcatcttcc ggcacagctt tccggcggtg aacagcaacg
     6361 agtggcgctg gcacgagcct ttaacggtcg acctgatgtg ctgtttgccg acgaacccac
     6421 cggcaatctt gaccgacaga cgggcgataa aattgccgac ctgctatttt ccctcaaccg
     6481 tgagcatggc accacgttga ttatggtgac ccacgacctg caactggcgg cacgctgcga
     6541 ccgctgctta cggctggtga acgggcaatt gcaggaggaa gcatgattgc acgttggttc
     6601 tggcgcgaat ggcgctcgcc gtcgctatta attgtctggc tggcgctaag cctggcggtg
     6661 gcctgcgtgc tggcgctggg caatatcagc gatcgcatgg agaagggctt aagccagcaa
     6721 agccgggagt ttatggcggg cgatcgggcg ttgcgcagtt cacgcgaagt gccacaagcg
     6781 tggctggagg aagcgcaaaa gcgcggcctg aaagtcggca agcagctgac tttcgccaca
     6841 atgacctttg caggcgacac accgcagctg gcgaacgtca aagcggtgga tgatatctac
     6901 ccgatgtatg gcgatctgca aactaatccc cctggcctga aaccgcaggc gggcagcgta
     6961 ttgctggccc cacgcctgat ggcactgctt aacctgaaaa caggcgacac catcgacgtg
     7021 ggtgatgcca ccttgcggat tgccggagaa gtgattcagg aaccggattc cggttttaac
     7081 cccttccaga tggctccgcg tctgatgatg aatctggcgg atgtcgataa aaccggagcc
     7141 gtgcagccgg ggagtcgggt cacctggcgc tataaattcg gcggcagcga gaaccagctc
     7201 gacggctatg agaaatggtt gttaccccag cttaaacctg aacaacgctg gtacggtctg
     7261 gaacaggacg aaggcgcgct ggggcgatcg atagaacgtt cgcaacagtt cctgctgctt
     7321 tcggcgcttc tgaccttgct gctggcggtg gcggcggttg cggtggcgat gaatcattac
     7381 tgtcgcagtc gctacgactt ggtggcgatc ctcaagacgc tgggggcagg gcgagcgcaa
     7441 ctgcgtaagt taatcgtcgg tcagtggttg atggtgctgg cgctttcagc cgttaccggt
     7501 ggtgcgatag gcctgttgtt cgaaaacgtg ttgatggtgc tgctcaagcc cgttttgcct
     7561 gctgcactac cgccagccag cctctggccg tggttgtggg cgctgggcac catgacggtc
     7621 atctcgctgc tggtggggct acgaccgtac cgcttgttgc tggcaacgca gcctttacgc
     7681 gtattacgta atgatgtggt cgcgaacgtc tggccgctga agttttatct gccgattgtc
     7741 agtgtggtgg ttgtgctgct gctcgccgga ttaatgggtg gcagcatgct gctttgggcg
     7801 gtgctggcgg gcgcggtagt actggctttg ctgtgcggtg tgctgggctg gatgctgctg
     7861 aatgtacttc gccgcatgac gctgaaatcg ctacctctgc gcctggcggt tagccgcctg
     7921 ttacgtcagc cgtggtcaac gttaagccag ctttcggcat tttcgctctc ctttatgctg
     7981 ctggcgctgt tgctggtatt gcgtggcgat ctgctcgacc gctggcagca gcagctacct
     8041 ccagaaagcc cgaactactt tttaattaac atcgccacag aacaggtagc accgctaaaa
     8101 gcgttcctcg cggaacatca gatagtcccg gaatcgtttt atccggtggt gcgggcgcgg
     8161 ctgacggcga ttaacgataa gccgacagaa ggcaatgaag atgaggcgct caatcgcgaa
     8221 ctcaatctta cctggcaaaa tacgcggccc gatcataatc cgattgtcgc cggtaactgg
     8281 ccgccaaaag ccgatgaagt gtcgatggaa gaggggctgg cgaaacgctt aaacgttgcc
     8341 ctcggtgata ccgtgacttt tatgggcgat acccaggagt tccgcgctaa agtgaccagc
     8401 ctgcgcaaag tggactggga aagtctgcgg cctaatttct attttatttt ccctgaaggg
     8461 gcattagacg ggcaaccgca gagctggctt accagtttcc gctgggagaa tggcaacggc
     8521 atgttgacgc aactcaaccg ccagttccct actattagcc tgttagacat tggcgcgatt
     8581 ttaaaacagg tcggtcaggt gctggagcag gtaagtcggg cgctggaggt gatggtggtg
     8641 ctggtcaccg cctgcggtat gttgctgttg ctggcacagg tgcaggtggg aatgcgtcag
     8701 cgtcatcagg agctggtggt gtggcgcaca ctcggtgcgg ggaaaaaact gctgcgtacc
     8761 acgttgtggt gtgagttcgc catgcttggg tttgtttctg gcctggtagc cgcaattggt
     8821 gcggaaacgg cgctggcagt gttgcaggcg aaagtgtttg attttccgtg ggagccagac
     8881 tggcgattgt ggattgtgct gccgtgcagc ggtgcactgc tgctgtcgct tttcggcggc
     8941 tggctgggtg cgcgactggt taagggtaag gcgctgttca ggcagtttgc gggatgatga
     9001 gagtaaataa ggggcgttct ggttgaatgg aacgccttgt ttaaattaat tctacaatat
     9061 gtttcattga tgatgttatt gaattggtga tttcctatct ttctattgct gatattaatc
     9121 tgaatcggtg gagtttaat
//
LOCUS       DAZFLD010000187         8963 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.188, whole genome shotgun sequence.
ACCESSION   DAZFLD010000187 DAZFLD010000000
VERSION     DAZFLD010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8963
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.188"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            170..1204
                     /gene="rhaT"
                     /locus_tag="ACHKBL_003899"
     CDS             170..1204
                     /gene="rhaT"
                     /locus_tag="ACHKBL_003899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015153 - rhamnose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008645 - hexose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
                     /protein_id="HFO5820701.1"
                     /translation="MSNAITMGIFWHLIGAASAACFYAPFKKVKKWSWETMWSVGGIV
                     SWIILPWAISALLLPNFWAYYSSFSLSTLLPVFLFGAMWGIGNINYGLTMRYLGMSMG
                     IGIAIGITLIVGTLMTPIINGNFDVLINTEGGRMTLLGVLVALIGVGIVTRAGQLKER
                     KMGIKAEEFNLKKGLVLAVMCGIFSAGMSFAMNAAKPMHEAAAALGVDPLYVALPSYV
                     VIMGGGAIINLGFCFIRLAKVKDLSLKADFSLAKPLIIHNVLLSALGGLMWYLQFFFY
                     AWGHARIPAQYDYISWMLHMSFYVLCGGIVGLVLKEWNNAGRRPVTVLSLGCVVIIVA
                     ANIVGMGMAN"
     gene            complement(1201..2049)
                     /gene="rhaR"
                     /locus_tag="ACHKBL_003900"
     CDS             complement(1201..2049)
                     /gene="rhaR"
                     /locus_tag="ACHKBL_003900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaR"
                     /protein_id="HFO5820702.1"
                     /translation="MAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDFCELVIV
                     WRGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKLNLDWQG
                     AIPGFSASAGQPHWRLGSVGMAQARQIIGQLEHESSQHVPFANEMAELLFGQLVMLLN
                     RHRYTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQFRQQTG
                     MTINQYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMTPSQWRH
                     LNSQKD"
     gene            complement(2123..2959)
                     /gene="rhaS"
                     /locus_tag="ACHKBL_003901"
     CDS             complement(2123..2959)
                     /gene="rhaS"
                     /locus_tag="ACHKBL_003901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaS"
                     /protein_id="HFO5820703.1"
                     /translation="MTVLHSVDFFPSGNASVAIEPRLPQADFPEHHHDFHEIVIVEHG
                     TGIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLL
                     PQEQDGQYPSHWRVNHSVLQQVRQLVAQMEQQEEENDLPSTASREILFMQLLLLLRKS
                     SLQENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQR
                     YLNRLRLMKARYLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGF
                     LQ"
     gene            3247..4716
                     /gene="rhaB"
                     /locus_tag="ACHKBL_003902"
     CDS             3247..4716
                     /gene="rhaB"
                     /locus_tag="ACHKBL_003902"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008993 - rhamnulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HFO5820704.1"
                     /translation="MTFRNCVAVDLGASSGRVMLARYERECRSLTLREIHRFNNGLHS
                     QNGYVTWDVDSLESAIRLGLNKVCEEGIRIDSIGIDTWGVDFVLLDQQGQRVGLPVAY
                     RDSRTHGLMAQAQRQLGKCDIYQRSGIQFLPFNTLYQLRALTEQQPELIPHIAHALLM
                     PDYFSYRLTGKMNWEYTNATTTQLVNINSDDWDESLLAWSGANKAWFGRPTHPGNVIG
                     HWICPQSNEIPVVAVASHDTASAVIASPLNSSRAAYLSSGTWSLMGFESQTPFTNDTA
                     LAANITNEGGAEGRYRVLKNIMGLWLLQRVLQERQINDLPALIAATQALPACRFTINP
                     NDDRFINPDEMCSEIQAACRETAQPIPESDAELARCIFDSLALLYADVLHELAQLRGE
                     DFSQLHIVGGGCQNALLNQLCADACGIRVIAGPVEASTLGNIGIQLMTLDELNNVDDF
                     RQVVSTTANLTTFTPNPDSEIAHYVAQIHSTRQTKELCA"
     gene            4713..5972
                     /gene="rhaA"
                     /locus_tag="ACHKBL_003903"
     CDS             4713..5972
                     /gene="rhaA"
                     /locus_tag="ACHKBL_003903"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HFO5820705.1"
                     /translation="MTTQLEQAWELAKQRFAAVGIDVEEALRQLDRLPVSMHCWQGDD
                     VSGFENPEGSLTGGIQATGNYPGKARNASELRADLEQAMRLIPGPKRLNLHAIYLESD
                     TPVARDQIKPEHFKNWVEWAKTNQLGLDFNPSCFSHPLSADGFTLSHADDSIRQFWID
                     HCKASRRVSAYFGEQLGTPSVMNIWIPDGMKDITVDRLAPRQRLLAALDEVISEKLDP
                     AHHIDAVESKLFGIGAESYTVGSNEFYLGYATSRQTALCLDAGHFHPTEVISDKISAA
                     MLYVPQLLLHVSRPVRWDSDHVVLLDDETQAIASEIVRHDLFDRVHIGLDFFDASINR
                     IAAWVIGTRNMKKALLRALLEPTAELRKLESAGDYTARLALLEEQKSLPWQAVWEMYC
                     QRHDTPAGSEWLENVRAYEKEILSRRG"
     gene            6157..6981
                     /gene="rhaD"
                     /locus_tag="ACHKBL_003904"
     CDS             6157..6981
                     /gene="rhaD"
                     /locus_tag="ACHKBL_003904"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HFO5820706.1"
                     /translation="MQNITQSWFVQGMIKATTDAWLKGWDERNGGNLTLRLDDADIAP
                     YHDNFHQQPRYIPLSQPMPLLANTPFIVTGSGKFFRNVQLDPAANLGIVKVDSDGAGY
                     HILWGLTNKAVPTSELPAHFLSHCERIKATNGKDRVIMHCHATNLIALTYVLENDTAV
                     FTRQLWEGSTECLVVFPDGVGILPWMVPGTDEIGQATAQEMQKHSLVLWPFHGVFGSG
                     PTLDETFGLIDTAEKSAQVLVKVYSMGGMKQTISREELIALGQRFGVTPLASALAL"
     gene            6991..7305
                     /gene="rhaM"
                     /locus_tag="ACHKBL_003905"
     CDS             6991..7305
                     /gene="rhaM"
                     /locus_tag="ACHKBL_003905"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0062192 - L-rhamnose mutarotase activity
                     [Evidence IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HFO5820707.1"
                     /translation="MIRKAFVMQVNPDAHEEYQRRHNPIWPELEAVLKSHGAHNYAIY
                     LDKARNLLFATVEIESEERWNAVASTDVCQRWWKYMTDVMPTNPDNSPVSSELQEVFY
                     LP"
     gene            complement(7346..8740)
                     /locus_tag="ACHKBL_003906"
     CDS             complement(7346..8740)
                     /locus_tag="ACHKBL_003906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HFO5820708.1"
                     /translation="MKKSTLSLAIGLLLACSTGMAKTQHLTLEQRMALLEERLEAAEM
                     RAAKAESQVKQLQTQQAAEIREIKAAQGNTPVNGQATSESAKKNSTSPNLLLSGYGDL
                     KIYGDVEFNMDAESNHGLLAMTNADVNSDPTNEQWNLNGRILLGFDGMRKMDNGYFAG
                     FSAQPLGDMHGSVNIDDAVFFFGKENDWKVKVGRFEAYDMFPLNQDTFVEHSGNTAND
                     LYDDGSGYIYMMKEGRGRSNAGGNFLVSKQLDNWYFELNTLLEDGTSLYNDGNYHGRD
                     MEQQKNVAYLRPVIAWSPTEEFTVSAAMEANVVNNAYGYTDSKGNFVDQSDRTGYGMS
                     MTWNGLKTDPENGVVVNLNTAYLDANNEKDFTAGINALWKRFELGYIYAHNKIDEFSG
                     VVCDNDCWIDDEGTYTIHTIHASYQFANVMDMENFNIYLGTYYSILDSDGDKKHGDDT
                     DDRYGARVRFKYFF"
BASE COUNT         2201 a         2304 c         2283 g         2175 t
ORIGIN      
        1 tatggttttg caggaaaatg cccgagatgt gaagcaaatc acccacttaa tgccgtgatt
       61 gccagtaaat cgacaacggc ggcaacaggc gaaaggttaa tcgacagcac gatttttaca
      121 ctcatctcgt cggagatgtg acgcgacgaa aaatgatgag gataagaaga tgagtaacgc
      181 gattacgatg gggatatttt ggcatttgat cggcgcggcc agtgcagcct gtttttacgc
      241 tccgttcaaa aaagtaaaaa aatggtcatg ggaaaccatg tggtcagttg gtgggattgt
      301 ttcgtggatt attctgccgt gggccatcag cgccctgtta ctaccgaatt tctgggcgta
      361 ttacagctcg tttagtctct ctacgctgct gcctgttttt ctgttcggcg caatgtgggg
      421 gattggtaat atcaactacg gcctgaccat gcgttatctc ggcatgtcga tggggattgg
      481 tatcgccatt ggtatcacgt tgattgttgg tacgctgatg acgccaatta tcaacggcaa
      541 tttcgatgtg ttgatcaata ccgaaggtgg acgcatgaca ttgctcggcg ttctggtggc
      601 gctgattggt gtagggattg tgactcgcgc cgggcagttg aaagagcgca agatgggcat
      661 taaagccgaa gagttcaacc tgaaaaaagg gctggtgctg gcggtgatgt gcggcatttt
      721 ctctgccgga atgtcctttg cgatgaacgc cgcaaaaccg atgcatgaag ccgctgccgc
      781 actgggcgtc gatccactgt atgtcgctct gccaagctat gttgtcatca tgggcggcgg
      841 cgcgatcatt aacctcggtt tctgcttcat tcgtctggca aaagtgaagg atttgtcgct
      901 aaaagccgac ttctcgctgg caaaaccgct gatcattcac aatgtgttac tctcagcact
      961 gggcgggttg atgtggtatc tgcaattctt tttctatgcc tggggccacg cccgcattcc
     1021 ggcgcagtat gactacatca gttggatgct gcatatgagc ttctatgtat tgtgcggcgg
     1081 tatcgtcggg ttggtgctga aagagtggaa caatgcaggt cgccgtccgg taacggtgtt
     1141 gagcctcggt tgtgtggtga ttattgtcgc cgccaacatc gtcggcatgg gcatggcgaa
     1201 ttaatctttc tgcgaattga gatgacgcca ctggctgggc gtcatcccgg tttcccgggt
     1261 aaacaccacc gaaaaatagt tactatcttc aaagccacat tcggttgaaa tatcactgat
     1321 taacaggcgg ctatgctgga gaagatattg cgcatgacac actctgacct gccgcagata
     1381 ttgattgatg gtcattccag tctgctggcg aaattgctga cgcaaaacgc gctcactgca
     1441 cgatgcctca tcacaaaatt tatccagcgc aaagggactt ttcaggctgg ccgccagccg
     1501 ggtaatcagc ttatccagca acgtttcgct ggatgttggc ggcaacgaat cactggtgta
     1561 acgatggcga ttcagcaaca tcaccaactg cccgaacagc aactctgcca tttcgttagc
     1621 aaacggcaca tgctgactac tttcatgctc aagctgaccg ataatctgcc gcgcctgcgc
     1681 catccccacg ctacctaagc gccagtgtgg ttgccctgcg ctggcactaa atcccggaat
     1741 cgccccctgc cagtcaagat tcagcttcag ccgctccggg caataaataa tattctgcaa
     1801 aaccagatcg ttaacggaag cgtaggagtg tttatcgtca gcatgaatgt aaaagagatc
     1861 gccacgggta atgcgataag ggcgatcgtt gagtacatgc agaccattac cgcgccagac
     1921 aattaccagc tcacaaaaat catgtgtatg ttcagcaaag acatcttgcg gataacggtc
     1981 agccacagcg actgcctgct ggtcgctggc aaaaaaatca tctttgagaa gttttaactg
     2041 atgcgccacc gtggctacct cggccagaga acgaagttga ttattcgcaa tatggcgtac
     2101 aaatacgttg agaagattcg cgttattgca gaaaaccatc ccgtccctga cgaatatcac
     2161 gcggtgacca gttaaactct cggcgaaaaa gcgtcgaaaa gtggttactg tcgctgaatc
     2221 cgcagcgata ggcgatgtca gtaacgctgg cctcgctgtg gcgtagcaga tatcgggctt
     2281 tcatcagtcg caggcggttc aggtaacgct gaggcgtcag ccccgtttgc tgcttaagct
     2341 ggcgatgcag cgtacgcaat gaaagagaaa attgatccgc cacggcatcc caattcacct
     2401 catcggcaaa atggtcctcc agccaggcca gaagcaagtt gagacgtgac gcgctgtttt
     2461 ccaggttctc ctgcaaactg cttttacgca gcaagagcag taattgcata aataagatct
     2521 cgcgactggc ggtcgagggt aaatcatttt cctcttcctg ctgttccatc tgtgcaacca
     2581 gctgtcgcac ctgctgcaac acgctgtggt taacgcgcca gtgagacgga tactgcccat
     2641 cctgctcttg tggcagcaac tgattcagcc cggcgagaaa ctgaaatcga tccggcgagc
     2701 gatacagcac attggtcaga cacagattat cggtatgttc atacagatgc cgatcatgat
     2761 cgcgtacgaa acagaccgtg ccaccggtga tggtataggg ctgcccatta aacacatgaa
     2821 tacccgtgcc atgttcgaca atcacaattt catgaaaatc atgatgatgt tcaggaaaat
     2881 ccgcctgcgg gagccggggt tctatcgcca cggacgcgtt accagacgga aaaaaatcca
     2941 cactatgtaa tacggtcata ctggcctcct gatgtcgtca acacggcgaa atagtaatca
     3001 cgaggtcagg ttcttacctt aaattttcga cgggaaacca cgtaaaaaac gtcgattttt
     3061 caagatacag cgtgaatttt caggaaatgc ggtgagcatc acaccaccac aattcagcga
     3121 attgtgaaca tcatcacgtt catctttccc tggttgccaa tgacccattt tcctgtcagt
     3181 aacgagaagg tcgcgaattc aggcgctttt tagactggtc gtaatgaaat tcagcaggat
     3241 cacattatga cctttcgcaa ttgtgtcgcc gtcgatctcg gcgcatccag tggacgcgtg
     3301 atgctggcgc gttacgagcg tgaatgccgc agcctgacgc tgcgcgaaat ccatcgtttt
     3361 aacaatgggc tgcatagcca gaacggttat gtcacctggg atgtggatag cctggaaagt
     3421 gccattcgcc ttggattaaa caaggtgtgc gaggaaggga ttcgtatcga tagcattggg
     3481 attgatacct ggggcgtgga ctttgtgctg cttgaccaac agggtcagcg cgtgggcctg
     3541 cctgttgctt atcgcgatag ccgcacccat ggtctgatgg cgcaggcaca acggcaactc
     3601 ggcaaatgcg atatttatca acgtagcggc atccagtttt tgcccttcaa tacgctttat
     3661 cagttgcgtg cgctgacgga gcaacaacct gaacttattc cacacattgc tcacgctctg
     3721 ctgatgccgg attacttcag ttatcgtctg accggcaaga tgaactggga atataccaac
     3781 gccacgacca cgcaactggt caatatcaat agcgacgact gggacgagtc gctactggcg
     3841 tggagcgggg ccaacaaagc ctggtttggt cgcccgacgc atccgggtaa tgtcattggg
     3901 cactggattt gcccgcagag taatgagatt ccggtggtcg ccgttgccag ccatgatacc
     3961 gccagcgcgg ttatcgcctc gccgttaaac agttcacgcg ccgcttatct ctcttctggc
     4021 acctggtcat tgatgggctt cgaaagccag acgccattta ccaatgacac ggcgctggca
     4081 gccaacatca ccaatgaagg cggggcggaa ggtcgctatc gggtgctgaa aaatattatg
     4141 ggcttatggc tgcttcagcg ggtgcttcag gagcggcaaa tcaacgatct tccggcgctt
     4201 atcgccgcga cacaggcact tccggcttgc cgcttcacca tcaatcccaa tgacgatcgc
     4261 tttattaatc ctgacgagat gtgcagcgaa attcaggctg cgtgtcggga aacggcgcaa
     4321 ccaatcccgg aaagtgatgc tgaactggcg cgctgtattt tcgacagtct ggcgctgctg
     4381 tatgccgatg tgttgcatga gctggcgcaa ctgcgcggtg aagatttctc acaactgcat
     4441 attgtcggcg gtggctgcca gaacgcgctg ctcaaccagt tatgtgctga tgcctgcggt
     4501 attcgggtga tcgccgggcc tgttgaagcc tcgacgctcg gcaatatcgg catccagtta
     4561 atgacgctgg atgaactcaa caatgtggat gatttccgtc aggtcgtcag caccaccgcg
     4621 aatctgacca cctttacccc taatcctgac agtgaaattg cccactatgt ggcgcagatt
     4681 cactctacac gacagacaaa ggagctttgc gcatgaccac tcaactggaa caggcctggg
     4741 agctggcgaa acagcgtttc gcggcagtag ggattgatgt cgaggaggcg ctgcgccaac
     4801 ttgatcgttt acccgtttca atgcactgct ggcagggcga tgatgtttcc ggttttgaaa
     4861 acccggaagg ttcgctgacc ggtgggattc aggctactgg taattatccg ggcaaagcgc
     4921 gtaatgccag cgagctacgt gccgatctgg aacaagctat gcggctgatt ccggggccga
     4981 aacggcttaa tttacatgcc atctatctgg aatcagatac gccagtcgcg cgcgaccaaa
     5041 tcaaaccaga gcacttcaaa aactgggttg aatgggcgaa aaccaatcag ctcggtctgg
     5101 attttaaccc ctcctgtttt tcgcatccgc taagcgccga tggctttacg ctttcccatg
     5161 ccgacgacag tattcgccag ttctggattg atcactgcaa ggccagccgc cgtgtttcag
     5221 cctattttgg cgagcaactc ggcacaccgt cggtgatgaa catctggatc ccggatggca
     5281 tgaaagatat caccgttgac cgtctcgccc cgcgccagcg tctgctggca gcactggatg
     5341 aggtgatcag cgagaagctg gatccggcgc atcatatcga tgccgttgag agcaaattgt
     5401 ttggcattgg cgcggagagc tacacggttg gctccaatga gttttacctg gggtatgcca
     5461 ccagccgcca gacggcgctg tgcctggacg ccgggcattt ccacccgact gaagtgattt
     5521 ccgacaagat ttccgccgcc atgctgtatg tgccgcagtt gctgctgcac gtcagccgtc
     5581 cggttcgctg ggacagcgat cacgtagtgc tgctggatga tgaaacccag gcgattgcca
     5641 gtgagattgt tcgtcacgat ctgttcgacc gggtgcatat cggcctcgac ttcttcgatg
     5701 cctctatcaa ccgcattgcc gcgtgggtca ttggtacacg caatatgaaa aaagccctgc
     5761 tacgtgcgtt actggaacct accgctgaac tacgcaagct ggaatcggcg ggcgattaca
     5821 ctgcgcgtct ggcgctactg gaagagcaga aatcgttgcc gtggcaggcg gtatgggaaa
     5881 tgtattgtca gcgtcacgat acgccagcgg gtagcgaatg gctggagaac gtgcgggctt
     5941 atgagaaaga aattttgagt cgccgcgggt aaacactgcc ggatgcggca caagtgcctt
     6001 atccggccta cgggtcggca atagttgtag gcctgataag acgcgacagc gtcgcatcag
     6061 gcattgattg ccggatgcgg cacgagtgcc ttatccggcc tacagatcgt atatccggca
     6121 acaggcacgg acgtccaatt aacaggaaca ccgaacatgc aaaacattac tcagtcctgg
     6181 tttgtccagg gaatgattaa agccaccacc gacgcctggc tgaaaggctg ggacgagcgc
     6241 aacggcggca acctgacgct gcgcctggat gacgccgata tcgcaccata tcacgacaat
     6301 tttcaccaac aaccgcgcta tatcccgctc agccagccca tgcctttact ggcaaataca
     6361 ccgtttattg tcaccggctc aggcaaattc ttccgtaacg tccagcttga tcctgcggct
     6421 aacttaggca tcgtaaaagt cgacagcgac ggcgcgggct accacattct ttgggggtta
     6481 accaacaaag ccgtccccac ttccgaactt ccggctcact ttctctccca ctgcgagcgc
     6541 attaaagcca ccaacggtaa agatcgggtg atcatgcact gccatgccac caacctgatc
     6601 gcccttacct acgtactgga aaacgacacc gcggtcttca ctcgccaact gtgggaaggc
     6661 agcaccgaat gtctggtagt gttcccggat ggcgtcggca ttttgccgtg gatggtgccc
     6721 ggcaccgacg aaatcggcca ggcgaccgca caggaaatgc aaaaacattc gctggtgttg
     6781 tggcccttcc acggcgtctt cggcagcggt ccgacactgg atgaaacatt cggtttaatc
     6841 gacaccgcag aaaaatcagc acaagtatta gtgaaggttt attcgatggg cggcatgaaa
     6901 cagaccatca gtcgtgaaga gctgatagcg ctcggccagc gtttcggcgt tacaccactc
     6961 gccagtgcgc tggcgctgta aggagcaaac atgatccgca aagcctttgt catgcaggtt
     7021 aaccccgacg cccacgaaga gtatcagcgt cgtcataatc caatctggcc ggaactggaa
     7081 gcagtgttga aatctcacgg tgcgcataac tacgccattt acctcgacaa agcgcgtaat
     7141 ctgctgtttg ccacggtaga gattgaatct gaagaacggt ggaatgcggt agccagtact
     7201 gatgtttgcc aacgttggtg gaaatatatg accgacgtta tgccaactaa cccggataac
     7261 agcccagtca gtagcgagct gcaagaagtg ttttacctgc cttaatgagt tgcggtgccg
     7321 gaaaatatcc agcaccgcga cttcatcaga agaagtattt aaagcgaacg cgtgcaccgt
     7381 aacggtcatc agtatcatca ccgtgttttt tatcaccgtc gctatccaga atggagtaat
     7441 acgtcccgag gtaaatatta aagttctcca tatccatcac attagcgaac tgataagacg
     7501 catgaatggt gtgaatggtg tacgtccctt catcatcaat ccagcagtcg ttatcacaaa
     7561 ctacaccgct gaattcatca atcttattgt gcgcgtagat ataacccagc tcgaaacgtt
     7621 tccacagcgc gttaatcccg gcagtgaaat ctttctcatt attagcatct aaataggcgg
     7681 tattaagatt aaccacgaca ccattttccg gatccgtttt caggccattc caggtcatgc
     7741 tcataccata gccggtacga tcggactgat cgacaaaatt acccttgcta tcggtataac
     7801 cataagcatt atttaccacg ttcgcttcca tcgctgcgga aacggtgaat tcttccgtcg
     7861 gcgaccaggc aattaccgga cgcagataag caacattttt ctgctgttcc atatcgcgtc
     7921 catgataatt accgtcgtta tataaagatg ttccgtcttc cagtaacgtg tttaattcaa
     7981 aataccagtt atcgagttgt ttgctgacga ggaaattacc gccagcgtta gaacgcccgc
     8041 ggccctcttt catcatatag atataaccgc tgccatcgtc ataaagatcg ttcgcagtat
     8101 taccggaatg ttcaacaaag gtatcctgat tcagcgggaa catatcgtag gcttcaaaac
     8161 ggcctacttt gaccttccag tcgttttctt tgccaaagaa gaataccgca tcatcgatat
     8221 ttactgaacc gtgcatgtcc cccagcggtt gtgcggagaa cccggcgaaa taaccgttgt
     8281 ccatctttcg cataccgtca aaacctaaca gaatacgacc attgagattc cactgttcat
     8341 tagtgggatc gctattcaca tcagcgttgg tcattgccag aaggccatga ttactttccg
     8401 catccatatt aaattctacg tcgccgtaga tttttaaatc gccataacct gaaagcaaaa
     8461 gattaggtga ggtggaattt ttctttgcag attccgacgt tgcctgtcca tttaccggcg
     8521 tattgccctg ggcagcctta atttcgcgga tctcagcggc ttgttgtgtc tgcagctgtt
     8581 taacctggct ctctgctttt gctgcccgca tttctgccgc ttccaggcgt tcttccagca
     8641 atgccatgcg ttgttccagc gttaaatgct gtgtttttgc cattccggta ctacatgcca
     8701 ataataaacc gatggctaaa gataatgtcg attttttcat tatttacgtt ccctgaaata
     8761 aaagagcccc tacgctttcc aaaaggaata cgcatataga gaaatataag gtgtgcactt
     8821 tcgaattttt aatccaactt cagcaggccg atggatagta tcccgccata taatatgagt
     8881 aaatccgctg atttcctgga acgtgaggaa tataacaata gtgccgtttt taactaaata
     8941 tcgtaaaaaa tattagatat gat
//
LOCUS       DAZFLD010000188         8751 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.189, whole genome shotgun sequence.
ACCESSION   DAZFLD010000188 DAZFLD010000000
VERSION     DAZFLD010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8751
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.189"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(291..1223)
                     /locus_tag="ACHKBL_003907"
     CDS             complement(291..1223)
                     /locus_tag="ACHKBL_003907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HFO5820709.1"
                     /translation="MDNDKIDQHSDEIEVESEEKERGKTIEIDEDRLPSRAMAIHEHI
                     RQDGEKELERDAMALLWSAIAAGLSMGASLLAKGIFHVELEGVPGSFLLENLGYTFGF
                     IIVIMARQQLFTENTVTAVLPVMQKPTMSNVGLLMRLWGVVLLGNILGTGIAAWAFEY
                     MPIFNEETRDAFVKIGMDVMKNTPSEMFANAIISGWLIATMVWMFPAAGAAKIVVIIL
                     MTWLIALGDTTHIVVGSVEILYLVFNGTLHWSDFIWPFALPTLAGNICGGTFIFALMS
                     HAQIRNDMSNKRKAEARQKAERAENIKKNDKNPA"
     gene            1517..2272
                     /gene="mlaA"
                     /locus_tag="ACHKBL_003908"
     CDS             1517..2272
                     /gene="mlaA"
                     /locus_tag="ACHKBL_003908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding lipoprotein MlaA"
                     /protein_id="HFO5820710.1"
                     /translation="MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNF
                     NVLDPYIVRPVAVAWRDYVPQPARNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTR
                     FFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRD
                     DGGDMADGLYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYF
                     QRHDFIANGGELKPQENPNAQAIQDDLKDIDSE"
     gene            complement(2334..3674)
                     /gene="fadL"
                     /locus_tag="ACHKBL_003909"
     CDS             complement(2334..3674)
                     /gene="fadL"
                     /locus_tag="ACHKBL_003909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid transporter FadL"
                     /protein_id="HFO5820711.1"
                     /translation="MSQKTLFTKSALAVAVALISTQAWSAGFQLNEFSSSGLGRAYSG
                     EGAIADDAGNVSRNPALITMFDRPTFSAGAVYIDPDVNISGTSPSGRSLKADNIAPTA
                     WVPNMHFVAPINDQFGWGASITSNYGLATEFNDTYAGGSVGGTTDLETMNLNLSGAYR
                     LNNAWSFGLGFNAVYARAKIERFAGDLGQLVAGQIRQSPAWLTPQGQALAATANGIDS
                     NTKIAHLNGNQWGFGWNAGILYELDKNNRYALTYRSEVKIDFKGNYSSDLNPAFNNYG
                     LPIPTATGGATQSGYLTLNLPEMWEVSGYNRVDPQWAIHYSLAYTSWSQFQQLKATST
                     SGDTLFQKHEGFKDAYRIALGTTYYYDDNWTFRTGIAFDDSPVPAQNRSISIPDQDRF
                     WLSAGTTYAFNKDASVDVGVSYMHGQSVKINEGPYQFESEGKAWLFGTNFNYAF"
     gene            4046..4330
                     /locus_tag="ACHKBL_003910"
     CDS             4046..4330
                     /locus_tag="ACHKBL_003910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HFO5820712.1"
                     /translation="MSKCSADETPVCCCMDVGTIMDNSDCTASYSRVFANRAEAEQTL
                     AALTEKARSVESEPCKITPTFTEESDGVRLDIDFTFACEAEMLIFQLGLR"
     gene            4511..5821
                     /gene="fadI"
                     /locus_tag="ACHKBL_003911"
     CDS             4511..5821
                     /gene="fadI"
                     /locus_tag="ACHKBL_003911"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003988 - acetyl-CoA C-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadI"
                     /protein_id="HFO5820713.1"
                     /translation="MGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPAVDLGKMV
                     VGELLARSEIPAEVIEQLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATS
                     FQAIANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLARVLVDVNKARTMSQRLKLF
                     SRLRLRDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWS
                     DGKLKEEVMTAFIPPYKQPLAEDNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLT
                     DGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTM
                     SDLTLLDMHEAFAAQTLANIQLLGSERFARDVLGRAHATGEMDDSKFNVLGGSIAYGH
                     PFAATGARMITQTLHELRRRGGGFGLVTACAAGGLGAAMVVEAE"
     gene            5821..7965
                     /gene="fadJ"
                     /locus_tag="ACHKBL_003912"
     CDS             5821..7965
                     /gene="fadJ"
                     /locus_tag="ACHKBL_003912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003857 - 3-hydroxyacyl-CoA dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0004300 - enoyl-CoA hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008692 - 3-hydroxybutyryl-CoA epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009062 - fatty acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
                     /protein_id="HFO5820714.1"
                     /translation="MEMASAFTLNVRLDNIAVITIDVPGEKMNTLKAEFASQVRAIIK
                     QLRENKELRGVVFISAKPDNFIAGADINMIANCKTAQEAEALARQGQQLMAEIHALPV
                     PVIAAIHGACLGGGLELALACHGRMCTDDPKTVLGLPEVQLGLLPGSGGTQRLPRLIG
                     VSTALEMILTGKQLRAKQALKLGLVDDVVPQSILLEAAVELAKQDRPSSRPLPVRERI
                     LAGPLGRALLFKMVGKKTEHKTQGNYPATERILEVVETGLAQGTSGGYDAEARAFGEL
                     VMTPQSQALRSIFFASTDVKKDPGSDAPPAPLNSVGILGGGLMGGGIAYVTACKAGLP
                     VRIKDINPQGINHALKYSWDQLEGKVRRRHLKASERDRQLALISGTTDYCGFAHRDLI
                     IEAVFENLELKQQMVAEVEQNCAAHTIFASNTSSLPIGDIAAHAARPEQVIGLHFFSP
                     VEKMPLVEIIPHAGTSAQTIATTVKLAKKQGKTPIVVRDKAGFYVNRILAPYINEAIR
                     MLTEGERVEHIDTALVKFGFPVGPIQLLDEVGIDTGTKIIPVLEAAYGERFSAPANVV
                     SSILNDDRKGRKNGRGFYLYGQKGRKSKKQVDPAIYPLIGTQGQGRLSAPQVAERCVM
                     LMLNEAVRCLDEQVIRSVRDGDIGAVFGIGFPPFLGGPFRYIDSLGAGEVVAIMQRLA
                     TQYGSRFTPCERLVEMSKRGESFWKTTATDLQ"
     gene            8168..8653
                     /gene="sixA"
                     /locus_tag="ACHKBL_003913"
     CDS             8168..8653
                     /gene="sixA"
                     /locus_tag="ACHKBL_003913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0101006 - protein histidine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HFO5820715.1"
                     /translation="MQVFIMRHGDAALDAASDSVRPLTTNGCDESRLMANWLKGQKVE
                     IERVLVSPFLRAEQTLEEVGDCLNLPSSAEVLPELTPCGDVGLVSAYLQALTNEGVAS
                     VLVISHLPLVGYLVAELCPGETPPMFTTSAIASVTLDESGNGTFNWQMSPCNLKMAKA
                     I"
BASE COUNT         2112 a         2106 c         2323 g         2210 t
ORIGIN      
        1 gagcgggata tgaaaattgc cgtggatcgg tttaaccgcg agtacaacaa aaaagtggga
       61 gtggagtaaa gaacatgccc cgtacggaag tgcggggcat gcttaacggg atgtgtacat
      121 aaatgggtac tgatatgacg attatttcgg tactttctgc atttaaatca atgagttaat
      181 acttgtattc attatatcca tttaactaag aggacaatgc ggcatgagta tacccgctaa
      241 tggactgcgg ggtaagtacg ctgccgctcg attgcttaaa ccctcgccat ttatgccggg
      301 tttttatcat ttttcttaat gttttccgca cgttctgctt tttggcgtgc ttctgctttg
      361 cgcttgttgc tcatgtcgtt acggatctgt gcatgactca ttaacgcgaa gataaaggtg
      421 ccgccgcaga tgttccccgc taaagtaggt agtgcgaagg gccagatgaa atcgctccag
      481 tgcagcgtac cgttaaacac cagatagagg atttcaacag aaccgacaac gatgtgagtg
      541 gtgtcaccca gggcaataag ccaggtcatc aatataatca ccacaatctt tgccgcaccc
      601 gctgcaggaa acatccaaac catagtggcg atcagccagc cggaaatgat cgcgttggca
      661 aacatctcgc tgggggtgtt cttcatcaca tccatgccga ttttgacaaa tgcatcgcga
      721 gtttcttcat tgaagattgg catatattca aatgcccacg ccgcaatacc tgtcccgaga
      781 atattaccca gcagcacgac gccccataac cgcataagta agccaacgtt gctcattgtc
      841 ggtttttgca tgacgggtag taccgcagtc acggtgtttt cggtaaataa ttgctggcgg
      901 gccataatga cgataataaa accaaaggta taaccgagat tctccagcaa gaagctgccc
      961 ggcacgcctt ccagttcgac atgaaatatc ccttttgcca gtagcgaagc gcccatcgac
     1021 agacccgccg caatggctga ccacagtagt gccattgcgt cgcgttccag ctctttttca
     1081 ccatcctggc ggatatgctc atgaattgcc atcgcccggg aggggagtcg atcttcatct
     1141 atttctattg ttttgccgcg ctctttttct tcgctctcaa cttcaatttc gtcgctgtgt
     1201 tgatcaattt tgtcgttgtc catggtctct tctcttgaat tagcacgtat agctaaagcg
     1261 tagcggcttt tttgctcgca actggcggga gttactctga aaatgtagaa aaggctgcgt
     1321 ttgccttttt ctgtttctat agaatcaagt agcctacagg gcggcgatta ccaggctatg
     1381 atcaaatcag caaatcaggg cgtctggaca tcagttgacg tgctgttaca atcgcccaca
     1441 cctaaacagg cggatacggt atcgttccgt catggatggc aaactgcata agccataaaa
     1501 aaacagggag acatttatga agcttcgcct gtcggcgctt gctctgggaa ctacgcttct
     1561 ggtggggtgt gcgagttccg gtacagatca gcaagggcgt tctgacccgc tagaagggtt
     1621 caaccgcacc atgtacaact tcaatttcaa tgtattagac ccgtatattg ttcgaccggt
     1681 cgctgtcgcc tggcgtgatt atgttccgca accggcgcgt aacggtttga gcaactttac
     1741 tggcaacctt gaagaacctg cggtgatggt taactacttc ttgcagggcg acccttatca
     1801 ggggatggtc cactttaccc gctttttcct gaacaccatt ttggggatgg gcggctttat
     1861 tgatgttgca gggatggcga acccgaaact gcaacggact gaacctcacc gcttcggtag
     1921 tacgcttggt cattatggtg tgggttatgg gccatacgtt cagttaccgt tctacggtag
     1981 cttcacgctg cgtgatgacg gtggtgatat ggcggatggt ctttacccgg ttctttcctg
     2041 gctgacctgg ccgatgtctg tgggtaaatg gacgcttgaa gggatagaaa ctcgtgcgca
     2101 gttgttggat tccgatggtc tgctgcgtca gtcgtccgat ccttatatta tggtgcgcga
     2161 agcgtacttc cagcgtcatg atttcatcgc taatggcggc gaactcaaac cgcaggaaaa
     2221 tccgaacgcg caagcgattc aggatgattt aaaagatatc gattctgaat aagaaacaaa
     2281 caaaaaaggt gagtcgcaaa actcaccttt tttcttatta agcgaacgcg ttatcagaac
     2341 gcgtagttaa agttagtacc gaacagccag gctttacctt cagactcgaa ctggtatggg
     2401 ccttcgttaa ttttcacgct ctgaccgtgc atataagaaa caccaacatc gactgaagca
     2461 tctttattaa atgcgtaagt cgtacctgca ctcagccaga aacggtcctg gtccggaatg
     2521 gagatagaac gattctgtgc cggaactggg ctgtcatcaa aggcgatacc ggtacggaag
     2581 gtccagttat catcgtagta ataagtggtt cccaacgcga tgcggtaagc atctttaaag
     2641 ccttcatgtt tctggaacag cgtgtcgcca ctcgttgagg tcgctttcag ctgctggaac
     2701 tgactccagc tggtgtaagc caggctatag tgaattgccc actgcggatc aacacggtta
     2761 taacccgaca cttcccacat ttcaggcagg ttcagcgtca gataacccga ctgcgttgcg
     2821 ccacctgtcg cggtaggaat tggcaaaccg tagttattaa aagccgggtt aagatcgctg
     2881 ctgtagttac ccttgaagtc aattttcact tcagagcggt aggtcaatgc gtagcggttg
     2941 tttttatcca gttcatacag gataccggcg ttccagccaa agccccactg gttaccgttc
     3001 aggtgagcga ttttggtatt actgtcgatg ccgttggcgg tagctgctaa tgcttgcccc
     3061 tgaggagtca gccaagcagg agattgccta atctgaccag ccaccagttg ccccagatcg
     3121 cctgcgaaac gttcaatttt cgcgcgagcg tagacggcgt tgaaaccaag accaaagctc
     3181 catgcattat ttaagcgata tgcaccgctt aagttcaggt tcatggtttc gaggtcggtg
     3241 gtaccaccga cagaaccgcc tgcataagta tcgttaaact ctgtcgccag accatagtta
     3301 gaggtaattg aagcccccca accaaattgg tcgttaatcg gtgcaacaaa gtgcatgttc
     3361 ggaacccatg ccgtaggcgc gatgttatcg gctttcaggc tacgaccaga cggagacgtt
     3421 ccgctgatat ttacatccgg atcaatataa accgcacccg cagaaaatgt cgggcggtca
     3481 aacatggtaa tcaatgcggg gttacggcta acgttacctg catcatcggc aattgcacct
     3541 tcccctgaat aagcccggcc caggccagag gaagaaaatt cgtttaactg aaagcctgcc
     3601 gaccaggcct gggtggagat aagtgccact gcgactgcga gagcagactt tgtaaacagg
     3661 gttttctggc tcatgaccat aacctcaatg atttattttt atacaaacta tgttacgtgc
     3721 tgtaacagga gcgcgaagtg tagggtctga ggtacatctt agaaatcaga ccagtggcga
     3781 gagtataggt cggaccagct ggaatgttgc aagtatgttt ctatcttttt tcagatatga
     3841 tctaagaaac gcgatccagc tggcaaaatt gggaggtggc gcactcatgg attcgggtga
     3901 attttgtttt agatcatttt taagtgtgat ttcggtcact tatccgattt tgcagaatta
     3961 acaactggag cagcactttc cggaagcgta aaatatccac aacgtttatc tggcaccttg
     4021 ggtgcacata cagaggaaat caacgatgag taaatgtagt gctgatgaaa ccccggtttg
     4081 ctgctgtatg gatgttggca ccattatgga caactccgac tgcaccgcgt cttacagccg
     4141 tgtgtttgca aaccgcgcag aagcagaaca aacgctggcg gcgctgactg aaaaagcccg
     4201 tagcgtggaa tccgaaccgt gcaaaatcac cccgactttc actgaagagt ctgacggtgt
     4261 gcgcctggat atcgatttca cttttgcctg cgaagctgaa atgctgatct tccagctggg
     4321 tctgcgttaa ttcctctcgg gtacatacgc ccctgtcagt tgctggcagg ggcgttttct
     4381 tttctcttct acttttctct ctgtgtcata gctcccactt tcccttttct ccacttggct
     4441 aaatgtaaaa aaatggttaa gactatgatc aggtcagacc actttattta ttattttaca
     4501 ggggagtgtt atgggtcagg ttttaccgct ggttacccgc cagggcgatc gtatcgccat
     4561 tgttagcggt ttacgtacgc cttttgcccg ccaggcgacg gcttttcatg gcattcctgc
     4621 tgttgatttg gggaagatgg tggtaggcga actgctggca cgcagcgaga tccccgccga
     4681 agtgattgaa caactggttt ttggtcaggt cgtacaaatg cctgaagccc ccaacattgc
     4741 gcgtgaaatt gttctcggta ccggaatgaa tgtgcatacc gatgcttaca gcgtcagccg
     4801 cgcttgcgct accagtttcc aggcgattgc aaacgtcgca gaaagcctga tggcgggaac
     4861 tattcgcgcg gggattgccg gtggggccga ttcctcttcc gtattgccaa ttggcgtcag
     4921 taaaaaactg gcgcgcgtgc tggttgatgt caacaaagct cgaaccatga gccagcgcct
     4981 gaaactcttc tctcgcctgc gtttgcgcga cttaatgccg gtgcctccgg cagtggcgga
     5041 atactctact ggcttgcgga tgggcgacac cgctgaacaa atggcgaaaa cctacggtat
     5101 tactcgcgaa cagcaagatg cattagcgca ccgttcgcat cagcgagccg ctcaggcgtg
     5161 gtcagacggt aaactcaaag aagaggtgat gactgccttt atccctcctt ataaacaacc
     5221 gcttgccgaa gacaacaata ttcgcggtaa ttcctcgctt gctgattacg caaagttgcg
     5281 tccggcgttt gatcgtaaac acgggacggt aacggcagca aacagtacgc cgctgaccga
     5341 tggcgcagcg gcggtgatcc tgatgaccga atcgcgggcg aaagaattag ggctggtacc
     5401 gctggggtat ctgcgcagtt acgcatttac tgcgatagat gtctggcagg acatgttgct
     5461 cggtccggcc tggtcaacgc cgctggcgct ggaacgtgcc ggtttgacaa tgagcgatct
     5521 gacattgctc gatatgcacg aagcctttgc agctcagaca ctggcgaata ttcagttgct
     5581 gggtagcgaa cgttttgctc gtgatgtact ggggcgtgca catgccactg gcgaaatgga
     5641 cgatagcaaa tttaacgtgc ttggcggttc gattgcttac gggcatccct tcgcggcgac
     5701 cggcgcgcgg atgattaccc agacattgca tgaacttcgc cgtcgcggcg gtggatttgg
     5761 tttagtgacc gcctgtgctg cgggtgggct tggcgcggca atggttgtgg aggcggaata
     5821 atggaaatgg catcagcgtt tacccttaat gtccgtctgg acaacattgc tgttatcacc
     5881 atcgacgtac cgggtgagaa aatgaatacc ctgaaggcgg agtttgcctc gcaggtgcgc
     5941 gccattatta agcaactccg tgaaaacaaa gagttgcgcg gcgtggtgtt tatctccgcg
     6001 aaaccagaca acttcattgc tggcgcagac atcaacatga tcgccaactg caaaacggcg
     6061 caagaagcgg aagcactggc gcggcagggc caacagttga tggcggagat tcatgctttg
     6121 cccgttccgg ttatcgcggc tattcatggc gcttgcctgg gcggtgggct ggagttggcg
     6181 ctggcgtgcc acggtcgcat gtgtactgac gatcctaaaa cggtgctcgg tttgcctgaa
     6241 gtacaacttg gattattacc cggttcaggc ggcacccagc gtttaccgcg cctgataggt
     6301 gtcagcacag cattggagat gatcctcact ggaaaacaac ttcgggcgaa acaggcatta
     6361 aagctggggc tggtggatga cgtcgttccg caatccattt tactggaagc cgctgttgag
     6421 ctggcaaagc aggatcgccc atcttcacgc cctctacctg tacgcgagcg tattctggcg
     6481 gggccgttag gtcgtgcgct gctgttcaaa atggtcggta agaaaacaga acacaaaact
     6541 caaggtaact atccggcgac agaacgcatc ctggaggttg tcgaaacggg attagcgcag
     6601 ggcaccagtg gcggctatga cgctgaagcc cgtgcttttg gcgaactggt gatgacgccg
     6661 caatcgcagg cgctgcgtag tatctttttt gccagtacgg acgtgaagaa agatcctggc
     6721 agtgatgcgc cgcctgcgcc attaaacagc gtgggaattt taggtggtgg cttgatgggc
     6781 ggcggtattg cttatgtcac tgcttgtaaa gcggggcttc cggtcagaat taaagatatc
     6841 aacccgcagg gcataaatca tgcgctgaag tacagttggg atcagctgga gggcaaagtt
     6901 cgccgtcgtc atctcaaagc cagcgaacgt gacagacagc tggcattaat ctccggaacg
     6961 acggactatt gcggctttgc ccatcgcgat ctgattattg aagcggtgtt tgaaaatctc
     7021 gaattaaaac aacaaatggt ggcggaagtt gagcaaaatt gcgccgctca taccatcttt
     7081 gcttcgaaca cgtcatcttt accgattggc gatatcgccg ctcacgccgc gcgacctgag
     7141 caagttattg gcctgcattt cttcagtccg gtggaaaaaa tgccgttggt agaaattatt
     7201 ccccatgccg gtacatcggc gcaaaccatc gccaccacag taaaactggc gaaaaaacag
     7261 ggcaaaacgc caattgtcgt gcgtgacaaa gccgggtttt acgtcaatcg catcttagcg
     7321 ccttacatta atgaagctat ccgcatgttg accgaaggtg aacgggtaga gcacattgat
     7381 accgcgctgg tgaaatttgg ttttccggta ggcccaatcc aacttttgga tgaggtagga
     7441 atcgacaccg ggactaaaat tattcctgta ctggaagccg cttatggaga acgttttagc
     7501 gcgcctgcaa atgttgtttc ttcaattttg aacgacgatc gcaaaggcag aaaaaatggc
     7561 cggggtttct atctttatgg tcagaaaggg cgtaaaagca aaaaacaggt cgatcccgcc
     7621 atttacccgc tcattggcac acaagggcag gggcgactct ccgcaccgca ggtagctgaa
     7681 cggtgtgtga tgttgatgct gaatgaagca gtacgttgtc tggacgagca ggttatccgt
     7741 agcgtgcgtg acggagatat tggcgcggta tttggcattg gttttccgcc atttctcggt
     7801 ggtccgttcc gctacatcga ttctctcggt gcgggtgaag tggttgcaat aatgcaacga
     7861 ctggccacgc agtatggttc ccgttttacc ccttgcgagc gtttagtcga gatgagcaag
     7921 cgtggggaaa gtttttggaa aacaactgca actgacctgc aataagaagg tcaaagctat
     7981 atgaatccgc gctgaatggc ggagtgttgc tcaaaatgta aacgcatatt gactatactt
     8041 acgccattga ggtaaaaaac agcgtttcat tcggtgaatg gataaggcac aatgccggcc
     8101 accgttttct ttctctggtt tcagatgaaa gaaaacgggc gaatctggtt aacaaaagcg
     8161 gtgcaatatg caagttttta tcatgcgtca cggcgacgca gccctcgatg ccgccagtga
     8221 ttcggttcgt cctctgacca ctaacggttg tgacgaatct cgcctgatgg cgaactggct
     8281 gaaaggtcaa aaagtggaaa tcgaacgcgt tctggtgagc ccgttcctgc gagccgagca
     8341 aacacttgaa gaagtagggg attgtctgaa cctgccttcc agtgcagaag ttctgccgga
     8401 gttaacgccc tgcggcgatg ttggtctggt cagtgcctat ttgcaggcgt tgaccaatga
     8461 aggtgttgct tcggtactgg tgatctccca cttaccctta gtcgggtatc tggttgccga
     8521 gttatgtccg ggcgaaacgc cgcctatgtt taccacctcg gccattgcca gcgtaacgct
     8581 tgatgaaagc ggaaacggca cattcaactg gcagatgagc ccgtgtaatc tgaagatggc
     8641 aaaagcgatt taatacgcat gcacaaggcc cgctggtgta atctatcggg cctttatttt
     8701 tttctggcaa tgttgaattt atgcaatctt attgtactaa caatgatccg c
//
LOCUS       DAZFLD010000189         8701 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.190, whole genome shotgun sequence.
ACCESSION   DAZFLD010000189 DAZFLD010000000
VERSION     DAZFLD010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8701
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.190"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            124..705
                     /locus_tag="ACHKBL_003914"
     CDS             124..705
                     /locus_tag="ACHKBL_003914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HFO5820716.1"
                     /translation="MVSLVKNQTVSLSKESPALSQLHFGLGWDPVKKKGFLGGLFGGN
                     DSIDLDAGCVLMDSTGKTIDTIWFRKLESTCKAVVHSGDNLTGEGDGDDEVIKVNLSR
                     LPANVEYLAFTVNSFRGQSFNDVENAFCRVVDQTGKELACYKLTEQGSHTGIVISSLR
                     RNNGNWDFTALGHACRGRTIDDMHSDIVSAVIR"
     gene            705..1862
                     /locus_tag="ACHKBL_003915"
     CDS             705..1862
                     /locus_tag="ACHKBL_003915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HFO5820717.1"
                     /translation="MNLMPGGNAPVPAQELRVRITSGVQVDASAFRLYAAGKVQGDAD
                     MVFYGQPRNDDGTVSLVSEGQYSTFTVALNRLKPDVQKIAFTVTCDGGQTVSGLRNLS
                     IDVEQGATGLVSGCVELSGRQEAALILGEFYRRNNGWKFRFVAQGFNGGLKPLAEHFG
                     VNIADEPAPAAPTPPVVPPLPAETKISLSKVSLTKEMPAISLTKRDNFGEIRINLNWH
                     RGSGKSGFAGMFGSKGIDLDLGAFVELQDGYKSVIQALGNAFGDYRDEPYVQLKGDDR
                     TGDVSDGEWLHINGREWKHIREVLIYAFIYEGVPSWDKTDGVVTIYVPDQPPIETRLT
                     EGENRRTLCAIARLVNENGAIKVERINQYFKGQDEMDRAFGWGFRWSAGSK"
     gene            1885..2340
                     /locus_tag="ACHKBL_003916"
     CDS             1885..2340
                     /locus_tag="ACHKBL_003916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HFO5820718.1"
                     /translation="MSFFDKVKGAINSGRDELTRQVGRFKNKKFMQGTVAVCARIAVS
                     SDGVSSEEKQKMMGFLRSSEELKVFDTNEVIEFFNKLVSSFDFDVEIGKGETMKYILA
                     LKDQPEAAQLALRVGIAVAKSDGNFDQDEKLASREIAIALGFEPAEFGL"
     gene            2359..3399
                     /gene="terC"
                     /locus_tag="ACHKBL_003917"
     CDS             2359..3399
                     /gene="terC"
                     /locus_tag="ACHKBL_003917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurium resistance membrane protein TerC"
                     /protein_id="HFO5820719.1"
                     /translation="MVSTHIGFPTETVIVFIALSVGAIFIDLFMHRDDKPISLKSAAL
                     WSVFWVVVAMAFAGFLYIHHGAEVASLFVTGYALEKVLSVDNLFVMMAIFSWFAVPDR
                     YRHRVLYWGIIGAIVFRGIFVAIGTSLLSLGPYVEVVFAIIVAWTAVMMLKSGDDDDE
                     IEDYSQHLAYRMVKRFFPVWPKLRGHAFLLNQKEVDAELAKPENSDVTIDRGKKAALY
                     ATPLFLCVAVVELSDVMFAFDSVPAIIAVSREPLIVYSAMMFAILGLRTLYFVLEALK
                     QYLIHLEKAVIVLLFFIAVKLGLNATDHIWHHGYSIAATTSLYVVLGVLVLGILASVI
                     FPGKPESEEKGN"
     gene            3448..4026
                     /gene="terD"
                     /locus_tag="ACHKBL_003918"
     CDS             3448..4026
                     /gene="terD"
                     /locus_tag="ACHKBL_003918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurium resistance membrane protein TerD"
                     /protein_id="HFO5820720.1"
                     /translation="MSVSLSKGGNVSLSKAAPSMKNVLVGLGWDARSTDGQDFDLDAS
                     AFLLTANGKVRGDSDFIFYNNLTSSDGSVTHTGDNRTGEGDGDDESLKIKLDTVPSDI
                     DRIIFVVTIHDAQARRQNFGQVSGAFIRLVNDDNQTEVARYDLTEDASTETAMLFGEL
                     YRHNGEWKFRAVGQGYAGGLASVCAQYGINAS"
     gene            4094..4669
                     /locus_tag="ACHKBL_003919"
     CDS             4094..4669
                     /locus_tag="ACHKBL_003919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HFO5820721.1"
                     /translation="MAVSLVKGGNISLTKEAPTMNVAMVGLGWDARVTDGQAFDLDAS
                     VFAVGEDGKVLSDAHFIFFNNKTSPDGAVEHQGDNRTGEGDGDDEQIKIDLAKVSVDV
                     KKLVFAVTIYDAEARKQNFGMVSNSFMRVYNNDNGAEIARFDLSEDASTETAMVFGEL
                     YRHGAEWKFKAVGQGFAGGLSALASQHGVNV"
     gene            4783..6077
                     /locus_tag="ACHKBL_003920"
                     /pseudo
     CDS             4783..6077
                     /locus_tag="ACHKBL_003920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418698.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            6126..7334
                     /locus_tag="ACHKBL_003921"
     CDS             6126..7334
                     /locus_tag="ACHKBL_003921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_025243830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFO5820722.1"
                     /translation="MDEKQLQALANELAKNLKTPEDLSQFDRLLKKISVEAALNAEMT
                     HHLGYEKNQSKPGTNSRNGYSTKTVITGDGPLELRTPRDRDGTFEPQLVKKNQTLITG
                     MDNQIFSLYAKGMTTREIAAAFKELYDADVSPALISKVTDAVMEQVVEWQNRPSDAVY
                     PIVYLDCIVLKVRQDSRVINKSVFLALGINIEGQKELPGMWLAENEGAKFWLNVLTEL
                     KNRGLNDILIACVDGLKGFPGAINTVYPKARIQLCIVHMVRNSLRFVSWKDYKAVTRN
                     LKAIYQAPTEEAGQQAQEAFAASWDSRYPHISRSWQANWPNLATFFAYATGIRKVIYT
                     TNAIESLNSVIRHAIKKRKVFPTDDSVKKVVWLAIQSASQKWTMPLKDWRMAMSRFII
                     DFGDRLGGHF"
     gene            complement(7449..8228)
                     /locus_tag="ACHKBL_003922"
     CDS             complement(7449..8228)
                     /locus_tag="ACHKBL_003922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000899430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HFO5820723.1"
                     /translation="MLFSRMMMTGVLSLMLAGCGNLSHVTDEGTTEEVVWPSPEKVMF
                     NHDGTQKGSWPLPEKLRQIKAGMNKDQIYQLAGRPHFAEGMSGVREWDYLFNFTGPEG
                     DYTCQYKILFDRNMDARSFYWKPENCLPEAETAEYRESVPGDILFDFDSWTLTERGRD
                     ALSRVAEKFQAGHVVGVKIEGFTDHLGGQAYNRLLSEKRAESVRGWFIARGFRAERIH
                     ATGRGYDAPVVFCPDVEGEALKECLRPNRRVEITGETSVTD"
     gene            complement(8253..>8701)
                     /locus_tag="ACHKBL_003923"
     CDS             complement(8253..>8701)
                     /locus_tag="ACHKBL_003923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011310120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YadA family autotransporter adhesin"
                     /protein_id="HFO5820724.1"
                     /translation="GSDTAVVSFGQPGQERQLQSVAPGVLSANSTDAVNGSQLHATNR
                     QVAGNTQAINTLGNKFSQLSYRVEELNRDIRGVGASAAAMSGIPQAYLPGKSLMGLGV
                     GGYGGESAIAIGVSRISDNGKMIMKLNAGQNTRGNFSVGAGVGWQW"
BASE COUNT         2106 a         2203 c         2256 g         2136 t
ORIGIN      
        1 gtgtttttac agaaagctac caaatttccc ttgtcagaga acttcatcat gatatgattt
       61 tcttcgccag ttactaaata tacttttgat tgtctcagtt atcagcattt taaggaataa
      121 aacatggttt cattagttaa gaatcagacg gtatcactca gcaaagaatc acctgcatta
      181 agccagcttc atttcggtct cggttgggat ccggttaaga aaaaaggttt tcttggagga
      241 ttgtttggag gtaacgactc aatcgatctc gatgctggct gtgtattaat ggacagcacc
      301 ggtaaaacaa ttgacaccat ctggttccgc aaactggaat ccacctgcaa agctgttgtg
      361 catagcggtg ataacctgac tggtgagggt gacggcgacg atgaagtgat taaggtcaac
      421 ctctcccgtc tgcctgcaaa cgttgaatac ctggcgttta cggttaacag cttccgtggg
      481 cagtcgttca atgacgtcga aaatgcgttc tgtcgtgtgg tcgatcaaac cggcaaagag
      541 ctggcttgct acaagctgac tgagcagggc tcacataccg ggatcgttat ttcttcgctg
      601 cgccgtaata acggcaactg ggactttacc gcccttggtc atgcctgtcg cggtcgcaca
      661 attgacgata tgcactccga tatcgtttct gcggttattc gctaatgaac ctgatgccgg
      721 ggggcaatgc ccccgttcca gcccaagagt tgcgggtccg gataacctcg ggcgtccagg
      781 tggatgcttc agcgtttcgc ctgtatgccg ccgggaaggt gcagggggat gcggacatgg
      841 tgttttatgg tcagccacgt aacgatgatg gtactgtcag cctggtcagt gaaggtcagt
      901 actcaacctt tactgtcgcg ctcaaccggc tgaaacctga tgtccagaaa attgcgttca
      961 ccgtgacctg tgacggtggg cagacggtgt ccggtcttcg taatctttcc atcgacgtgg
     1021 agcaaggggc taccggcctg gtgagcggct gtgtcgaact gagcggccgt caggaagcag
     1081 ccttaattct gggtgaattt taccgtcgta ataatggctg gaaattccgc ttcgttgcgc
     1141 aggggttcaa cggtggactt aaaccgctgg cagagcattt cggcgtaaat attgccgacg
     1201 aaccggctcc cgcagccccg actcctccgg ttgtaccccc tctgccagct gagacaaaaa
     1261 tcagcctgag taaagtatct ctgacaaaag agatgcctgc aatcagtctg accaaacggg
     1321 ataacttcgg tgaaatcagg atcaacctga actggcaccg gggcagcggc aaatccggct
     1381 ttgcaggcat gttcggctcc aaaggcatcg acctggatct gggagccttt gttgagcttc
     1441 aggacgggta taagtcggtc attcaggcgc tcggtaatgc attcggtgat tatcgcgatg
     1501 aaccttatgt tcagctcaag ggcgatgacc ggacgggtga tgtatcagac ggcgagtggc
     1561 tgcatatcaa tggacgtgaa tggaagcata tccgcgaagt gctgatttac gcctttattt
     1621 atgaaggtgt tcccagttgg gacaagactg atggtgtggt caccatttat gtgccggatc
     1681 aaccgcccat tgagacccgc ctgactgaag gtgaaaaccg tcgcacattg tgcgccattg
     1741 ccagactcgt aaacgaaaac ggtgcgatta aagtcgagcg tatcaaccag tacttcaaag
     1801 gccaggacga aatggaccgg gcatttggct ggggatttcg ctggagcgcc ggttctaaat
     1861 aacacagcaa taaaggaaac tggtatgagc tttttcgaca aagttaaagg tgccattaac
     1921 tctggccgtg acgaactgac ccgccaggtt ggtcgtttca aaaacaaaaa attcatgcag
     1981 ggcaccgttg ctgtatgtgc ccgtattgcc gtatcgagtg acggcgtgag ttcggaagaa
     2041 aagcagaaaa tgatgggctt tctgcgctct tcagaagagc tgaaggtctt cgataccaat
     2101 gaggtgatcg agttcttcaa taaactggtt tcaagcttcg atttcgatgt tgaaatcggc
     2161 aagggcgaga ccatgaaata catcctggcg ctgaaagatc agcctgaggc cgctcaactg
     2221 gccttacgtg ttggtattgc tgttgcgaaa agcgacggta acttcgatca ggacgagaaa
     2281 ctggcctccc gcgagatcgc tatcgcgttg ggcttcgagc cggctgaatt tggcctctga
     2341 catccctaag ggtaaaatat ggtttccaca cacatcggct tcccgactga aacggtcatt
     2401 gtttttattg ctctttcagt cggagccatc tttattgacc tgtttatgca ccgtgatgac
     2461 aagccaatct ccctgaagag tgcagcgctc tggtccgtat tctgggttgt tgttgcgatg
     2521 gcatttgccg gtttcctcta tatccaccac ggtgctgaag ttgccagttt gtttgtcacg
     2581 gggtatgcgc tggagaaagt actgtcggtc gacaacctgt ttgtcatgat ggccatcttt
     2641 tcctggttcg ctgttccgga tcgttatcgg caccgtgtgc tttactgggg gatcattgga
     2701 gcgatcgtct tcaggggaat ctttgtcgcc atcggtacga gcctgctgag tctggggccg
     2761 tacgttgaag tcgtcttcgc tatcatcgtt gcctggacag cggtcatgat gcttaaaagc
     2821 ggtgatgatg atgatgaaat tgaagattac tcccagcatc tggcttaccg catggttaaa
     2881 cgtttcttcc ctgtctggcc gaagctacgt gggcatgcct tcctgcttaa ccagaaggaa
     2941 gtggatgctg aactggcgaa accagaaaac agtgatgtaa ccattgaccg tggtaaaaag
     3001 gcggcgctgt atgcgacccc tctgttcctg tgtgtggccg tggttgaact ctcggatgtt
     3061 atgtttgcgt ttgactcggt accggcaatc attgctgtca gtcgtgaacc gcttatcgtc
     3121 tatagtgcca tgatgtttgc tatcctgggg ctgcgtactc tgtactttgt ccttgaagca
     3181 ctgaaacagt acctgattca tctggaaaag gctgttatcg tgttgttgtt cttcattgcg
     3241 gtaaaactgg gcctgaacgc gaccgatcac atctggcatc atggttacag catcgctgcg
     3301 acaaccagcc tgtatgttgt actgggggtt ctggtgctgg gcattctcgc aagcgttatt
     3361 ttccccggca aacctgaatc tgaggaaaag gggaactgat ccccttaagt gttaaccgga
     3421 gcaattacta atcgaagagg ttattttatg agtgtttctc tttccaaagg cgggaacgtc
     3481 tccctgagta aagcagctcc gtcaatgaaa aacgtcctgg tgggtcttgg ctgggatgca
     3541 cgttcaactg acggtcagga ctttgacttg gatgcttcag cattcctgct gaccgcaaac
     3601 ggcaaagtgc gtggcgattc agatttcatc ttctataaca acctgacgtc atccgacggt
     3661 tctgtaacgc acaccggcga taaccgtact ggtgaggggg atggtgatga tgaatcgctg
     3721 aaaattaaac ttgatactgt cccgtctgac atcgacagga tcatcttcgt tgtgacgatc
     3781 catgatgctc aggctcgccg tcagaacttt ggtcaggttt ccggggcgtt tattcgcctg
     3841 gttaatgacg ataaccagac tgaagttgct cgctacgatc tgactgaaga tgcgtccact
     3901 gaaaccgcca tgctgttcgg tgaactgtat cgtcacaatg gtgagtggaa attccgtgct
     3961 gtaggtcagg gttatgcggg ggggctggca tctgtatgtg ctcagtacgg cattaacgcg
     4021 tcttgatcga aaactaactt ttcattccgg gctgttgatt cagccctcat tcaactgttt
     4081 gcaggagcta aaaatggcag tttctctcgt aaaaggcggc aacatatctc tgaccaaaga
     4141 agcaccgacc atgaatgttg ctatggttgg tcttggctgg gatgcccgtg taactgacgg
     4201 tcaggctttt gatctggacg cttccgtatt cgcagtaggt gaagacggta aagtgctgtc
     4261 agatgcccat ttcattttct tcaataataa aaccagccct gatggcgcag tagagcacca
     4321 gggtgacaac cgtaccggtg aaggcgatgg tgacgatgag caaattaaaa tcgatctggc
     4381 aaaagtctca gttgacgtca aaaaactggt atttgccgtt accatctatg atgcagaagc
     4441 gcgtaaacaa aatttcggca tggtgagcaa cagcttcatg cgcgtttaca acaacgataa
     4501 cggcgcggaa attgcccgtt tcgatctgtc tgaagacgcc tcaactgaaa ccgcaatggt
     4561 cttcggtgaa ctgtatcgcc atggcgctga gtggaagttt aaagctgtcg gtcagggttt
     4621 tgccggtggc ctgtcagcgc ttgcttccca gcacggcgtc aacgtctaaa agatgcttag
     4681 tctgaccccg gcaatgccgt aatgccgatc ggttaaggat cagttgaccg atccagtggt
     4741 tgtgtaagaa tccggaaacg ctcactcgtt tccggatttt ttatgcacac tggacaggcc
     4801 cttgatctgg tatcccgtta cgattctctg cgtaacccac tggcttctct gggggattac
     4861 ctcgaccccg aactcatctc tcgttgcctt gccgaatcag gtacggtaac gctacgcaag
     4921 cgccgtcttc ccctcgaaat gatggtctgg tgtattgttg gcatggcgct tgagcgtaaa
     4981 gaaccccttc accagattgt gaatcgcctg gacatcatgc tgccggcaat cgccccttcg
     5041 ttgcccccag tgccgttatt caggcccgcc agcacctggg aagtgaggct gtccgccgcg
     5101 tgttcacgaa aacagcgcag ctctggcata acgccacgcc gcatccgcac tgatgcggcc
     5161 tgaccctgct ggccatcgat ggtatgttct ggcgcacacc ggatacacca gagaacgatg
     5221 cagccttccc ccgccagata catgccggca acccggcgct ctacccgcag gtcaaaatag
     5281 tctgccagat ggaactgacc agccatctgc ttacggctga gccttcggca cgatgaagaa
     5341 cagcgaaaat gagcttgctg agcaacttat agaacaaacc ggcgataaca ccctgacgtt
     5401 aatggataaa ggttattact cactgggact gttaaatgcc tggagcctgg tgggagaaca
     5461 ccgccactgg atgatccctc tcagaaaggg agcgcaatat gaagagatca gaaaactggg
     5521 taaaggcgat catctggtga agctgaaaac cagtccgcag gcacgaaaaa agtggccggg
     5581 actgggaaat gaggtgacag cccgcctgct gaccgtgacg cgcaaaggag aagtctgcca
     5641 tctgctgacg tcgatgacgg acgccatgcg cttccccggg ggagaaatgg cggatctgta
     5701 cagtcatcgc cgggaaattg aactgggata cagggagata aaacagacga tgcaactgag
     5761 caggctgacg ctgtaaaaag ccggagcttg tggagcaaga gctgtggggt gtcttactgg
     5821 cttataatct ggtaagatgt cagatgatta aaatggtgga acatctgaaa ggttactggc
     5881 cgaatcaact gagtttctca gaatcatgcg gaatggtgat gacattgcag ggcgcttcac
     5941 cgggacgtat accggggctg atgcgcgatc ttgcaagtat gggacaactt gtgaaattac
     6001 cgacaagaag ggaaaggggc ttcccgagag tggtaaagga gaggccttgg aaagagcctg
     6061 tacatagatt tgtgtaattg cctgattttg atatgttcaa tcaagcatca aatgaaggtt
     6121 aatttatgga cgaaaaacag ttacaggctc tggctaacga actggccaaa aacctcaaaa
     6181 cccctgaaga cctcagccag tttgaccgtc tactgaaaaa gatcagcgtt gaagccgctc
     6241 tcaatgcaga gatgacacac catcttgggt atgagaaaaa tcagtccaaa ccgggaacta
     6301 actcccgcaa cggttattcc acaaagaccg ttatcacagg cgacggtcca ctggaactgc
     6361 gtactccgcg cgatcgtgac ggtaccttcg aaccacaact ggtaaagaaa aatcagaccc
     6421 ttattactgg gatggataac cagatcttct cgttgtatgc caaagggatg accacccgtg
     6481 agatcgctgc tgcgttcaaa gagctgtatg acgcagatgt ttcaccggca ctgatatcaa
     6541 aggtcaccga tgccgtgatg gagcaggttg tagaatggca aaaccgaccg tcggatgctg
     6601 tttaccccat tgtttatctt gactgtatcg tccttaaagt tcggcaggac agtcgcgtca
     6661 ttaacaaatc ggtgttcctg gcactgggca tcaatatcga aggtcagaaa gaactgccag
     6721 gtatgtggct ggccgaaaat gaaggggcga agttctggct caatgtgctg actgaactga
     6781 aaaaccgcgg tctgaacgat atcctcatcg cctgtgtgga tggcctgaaa ggcttcccgg
     6841 gggccatcaa cacagtatat ccgaaggccc gcatccagtt atgcatcgtg catatggtgc
     6901 gcaacagcct gcgcttcgtg tcatggaagg actacaaagc cgtcactcga aacctgaaag
     6961 cgatttatca ggctcccacg gaagaggccg gccagcaggc acaggaagcg ttcgctgcgt
     7021 cctgggacag tcgctatcct catataagcc gaagctggca ggctaactgg ccgaatcttg
     7081 ccacgttctt cgcttatgcg acgggcatcc gcaaagtgat ctatacgacg aatgccatcg
     7141 agtcgctaaa cagcgtgatc cgccatgcga tcaaaaagcg taaagtgttc ccgacagacg
     7201 actcggtgaa aaaagtggta tggctggcaa tccagtcagc gtcccagaaa tggacgatgc
     7261 cgttgaagga ctggcgtatg gcaatgagcc gctttattat cgacttcggt gaccgcctgg
     7321 gtggtcactt ctgagaaagg gcatttacac agattcctaa acaggctccc tggaaatacc
     7381 ccacagcccc gaaaaagagc cagtcagttg ctgaactgac tggcattggc tcctggggca
     7441 ctttattgtt aatcagtcac tgatgtttcc ccggttattt caaccctgcg gttggggcgc
     7501 agacattcct tcagtgcttc gccctccaca tccgggcaga acaccactgg tgcatcatat
     7561 ccacggcccg ttgcatgtat cctttcagcc ctgaaacccc gggcaataaa ccatcccctg
     7621 actgattccg cccgtttctc tgacaggagc cggttatacg cctgcccgcc aagatggtcc
     7681 gtaaatcctt ctatcttcac cccgacaaca tgacccgcct gaaacttctc tgccacacgg
     7741 gaaagcgcat cacgccccct ctctgtcaga gtccatgaat caaaatcaaa taatatatca
     7801 ccggggactg actccctgta ttctgctgtc tctgcctccg gcagacagtt ttccggcttc
     7861 cagtagaaag agcgggcatc catgttccgg tcaaacagta tcttgtactg gcaggtataa
     7921 tccccctccg gtccggtaaa gttgaacagg taatcccatt cccttacacc agacatccct
     7981 tcagcaaagt gtggtctccc cgccagctga taaatctggt ccttattcat acccgccttt
     8041 atctgacgta acttttctgg cagtggccac gaacctttct gtgttccgtc atgattaaac
     8101 atcaccttct ccggagaagg ccataccact tcttccgtcg ttccttcatc cgtgacatga
     8161 ctcagatttc cacatcctgc aagcatcagg gacagcacac ccgtcatcat cattcgacta
     8221 aataacatta catttctcct tgcttttccc tgttaccact gccagcccac accggcaccg
     8281 acggagaaat taccccgggt gttctgacct gcattcagtt tcattatcat cttaccgtta
     8341 tcggagattc gggacacacc tattgcaatg gcgctttcac caccatatcc accgacaccc
     8401 agccccatca gacttttacc cggaagatag gcctgaggta tcccgctcat ggccgcagcc
     8461 gatgcgccaa caccacggat atcccggtta agctcttcca cccggtagct cagctgactg
     8521 aatttattcc cgagagtgtt tatcgcctgt gtatttccgg caacctgacg gttagttgca
     8581 tgcaactgac tgccattgac ggcatccgtt gaattcgcag acaacacacc cggtgccact
     8641 gactgcagct gacgctcctg tcccggctga ccaaaagaaa ccaccgcagt atcacttccg
     8701 g
//
LOCUS       DAZFLD010000190         8678 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.191, whole genome shotgun sequence.
ACCESSION   DAZFLD010000190 DAZFLD010000000
VERSION     DAZFLD010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8678
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.191"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..824
                     /locus_tag="ACHKBL_003924"
     CDS             <1..824
                     /locus_tag="ACHKBL_003924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000730426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TcpQ domain-containing protein"
                     /protein_id="HFO5820725.1"
                     /translation="TALPPRPLLQATQLPALVMTGKPGQSSVVYLREARQRSVSDWIR
                     QLLPPGWQFRQQNAAIPALNKRLPVWSVNDQWHRALNTLLTGQQLYGHMDWNRKILTV
                     TTTAAPPVDLTAPQGSQKTADTPRNPFRSTTGTAADQAQVPPAVSASRVQSTTGTSGT
                     SAPRTTTPAPVVTKPVAPPQKVTPPRQWKMEKGTTLKDGLARWTSSEKCPHGTWTLRW
                     ETPVNYSIDAPLVFTGSFRQALEQLFALYTPVEKPLYARTSTAQCLLRVTDRPQE"
     gene            829..1266
                     /gene="pilM"
                     /locus_tag="ACHKBL_003925"
     CDS             829..1266
                     /gene="pilM"
                     /locus_tag="ACHKBL_003925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001681791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis protein PilM"
                     /protein_id="HFO5820726.1"
                     /translation="MGWFIVFIMVITTTGNFWFTSQLSRSEHRHQAAENTQQAATFIR
                     YMNAINDYLHQHPERRTAGGRLTSTQLGIPGTDTVSHIISQQRVFVWATETPGLMAAL
                     REQCNDSALLGRVENGRLLDTAGRALSVTLPSVIPDHVILWMN"
     gene            1297..2916
                     /locus_tag="ACHKBL_003926"
     CDS             1297..2916
                     /locus_tag="ACHKBL_003926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilN family type IVB pilus formation outer
                     membrane protein"
                     /protein_id="HFO5820727.1"
                     /translation="MNKKILAIMIAAALLQGCASYRQLESTRKEAVAEQQRAREMMSD
                     LRTRTPVVKESSRQWINPRPVMDKNTGKTVPGCPIVIHTRQSITLQQVVQRITESCHI
                     PVRITPDVLAYLNTSTTGSTQQIQGALPPPDASGMVPLAAIGASDTPPSRLPGNTAAL
                     SELNASGLTTLLNAVSSRLGISWRYDGSHIIFYYLETQTFPITYMDSQVAYNATVVSG
                     TMSSNGSSGGSASDAISGDASNTQTTTVDMKSALYQDVKNAVTSMLTPGIGRMFMSTG
                     FITVTDNPQVLETVRAFIEKRNEEMKRQVVLNVEILSIRKTRNEQAGIDWNAVFSDRT
                     LGLSLGSTFTSAASDAITGGVSIVDGKLTGSKAFLKALSSQGDVSVVTQNSAVTKNLT
                     PVPMQIANQQSYIESVTTDTTANVGSSTSLNAATITTGFNMTLLPFILPDSQTLQLLY
                     SMSLSDKPVIENYESGGSKAQLPNVDLKTINQTVDLKSGQTVIISGFQQSGRRSGKQG
                     VGTPGFFGLGGGINSENDDTILVVLITPNII"
     gene            2937..4232
                     /gene="pilO2"
                     /locus_tag="ACHKBL_003927"
     CDS             2937..4232
                     /gene="pilO2"
                     /locus_tag="ACHKBL_003927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001681793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4b pilus protein PilO2"
                     /protein_id="HFO5820728.1"
                     /translation="MTDISPTERQMFPLTAGRNVFLAGLEWKTLPSQYRHARDFARAQ
                     KADLFLACQYLSNEDANTHTMVATASRRILPGKPRQYLSLALLILPLLEQGGYAITEL
                     TLPGETPRYSFVSAVDGVLVSDLVGSGEEVREARDTFLSINTEPEQGWTRYEPVAFSA
                     GDQNQALPLSTLTGAGKHPAAARLNPVSRGAQFITVSLIIALLGAAWYGWQYYERWKT
                     EQAAQAAAARQVTEEQISPPWPGLPETDAFIQGCADIWTSLPLSAAGWRYSLAECSTD
                     GGSGNLRASYTNTAGATVTDFIRRISDMTGTRPFIVMPEGKTGGIGLPVSFRLPEHPV
                     DVDALPESSVLQEQLTTLAQSMPLTLDWQEVSNSVTDENGNIIQPPWKEYDLQIQTLL
                     PAYQLVERLKAPSVRFISVTRQLENGRFHYQFTGKYYAR"
     gene            4222..4674
                     /gene="pilP"
                     /locus_tag="ACHKBL_003928"
     CDS             4222..4674
                     /gene="pilP"
                     /locus_tag="ACHKBL_003928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000543062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis protein PilP"
                     /protein_id="HFO5820729.1"
                     /translation="MHAKTKALLVSALLLPATAMATTGNAEEQSPRQLQIEQIQDETA
                     LYEAMLAREKIRHELRMLEQGIPAEPGRPQESTSAIPTAPSTAASPRLTEIFGVQPQL
                     SARIELSGGHITDVKTGQNIPGTPYLVEKITPDSVVLEQSGIRKTLRP"
     gene            4723..5277
                     /locus_tag="ACHKBL_003929"
     CDS             4723..5277
                     /locus_tag="ACHKBL_003929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001026779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5820730.1"
                     /translation="MNISELISWLSLIIRDLETAAAEYGVNHTDIVHEATQLQEQLCR
                     GKQVTPAQLRALSARLWGARMRLAAQYGQDAPLMNDLAFLSNCLKYDADRLNDRWRYR
                     EWISAAESFVLPLVFIIPLLIVLCYMMKSGNSGGAELCAALAGAWCTGLTFLCLWAKD
                     PVGLFWSLYSFIPLYFLWCDISPA"
     gene            5373..5969
                     /locus_tag="ACHKBL_003930"
     CDS             5373..5969
                     /locus_tag="ACHKBL_003930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000892312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5820731.1"
                     /translation="MLENKTTDDFIFVKQHIYRIFQWMHETPLINNGNTPVTDRLIAD
                     VRWMLEHHYYPSPGQVDRIISVLRETKLQLLREKTDRQELVEEIQQKLNTLKDIRHFL
                     KLNTNYKQDGDIISLTPSGEWKNTSIILSVVAVMLMIFASICNLSGITPYFIYTAGII
                     FYALATPVLTLRSTQPEKNIPLLFAFGGVIIFTLLSLC"
     gene            6066..7574
                     /locus_tag="ACHKBL_003931"
     CDS             6066..7574
                     /locus_tag="ACHKBL_003931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HFO5820732.1"
                     /translation="MLESADLPPDDILDYVAIDTDKTGHLRVRVVEGKKHLRAVQEYL
                     TRLRARHQGRVGDFEFTTLDVIARLRQDTTTAGDESVINPVQQKMLGYIRHSASLGAS
                     DLHMTPGRDNTDFTYVEARVHGELEVLDILRKEEGLELLGATYSGMTDVIKGTQFDPG
                     VPQDARLSEQYLKLAGLFGARYSHYPCVGGLYAVLRLIKDDSQQIPTFSMLGYHPDQE
                     RTLRRILQRPEGIITLSGPTGSGKSTTLRTASAAYLEQYGFNNTGGILLPRRRLFTIE
                     SPPEGRIPGAIQTAVMDTAQGWVDSIKSALRLDPDGILNGEIRDHDSAITAIKAAMTG
                     HLMLTTIHANDPINILERLEMEGVQARMIADPQLFIGLLSQRLVQLICPHCRRPWHEV
                     ATERTDDERRLVESVCNPDQVYLRNHEGCPHCWRGVTGRTVIAEVISPDARFFQLYRE
                     KGRIEARTYWHRELGGMTRNQHLLGKINSGQVDPLAAHYISPIDEDSYTLLH"
     gene            7576..8676
                     /locus_tag="ACHKBL_003932"
     CDS             7576..8676
                     /locus_tag="ACHKBL_003932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000670135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HFO5820733.1"
                     /translation="MRFLEKLNNTDIELTALTVWIARKTFALNRVEFYEALGGMIRDG
                     TPSLRALEFLCEIETDFGEKKGTSGMYFLAKECIDSIKASGMLSGALLTWVPPEEATL
                     IRNGEERGNIADALFQVARTVKARREMTSSLIAVCLYPLLLLSLCVVNMYNAHTRIIP
                     IVSAFASEDKWSFQMKLLAGMSEFFIGYGFWLCGTIFALTLLIRFSFSRYTGPGRRML
                     DKAPPWSLYQTLHGVNYLFSISSMLQINIPLSQALLRMEKGAEKNKWLKERIQAIRKH
                     VLSGQNLATAMRNSGYDFPSRICINKLLLNSEREDSVATMALYADQWLEEAKKRVKRI
                     GLIITGISGGFVFFFILNMISAIYSISDMLKQ"
BASE COUNT         2134 a         2492 c         2204 g         1848 t
ORIGIN      
        1 gtaccgcact gcctccccgc ccgctccttc aggccacaca gctgcccgca ctggttatga
       61 ccggtaaacc cggtcagtca tccgtcgttt atttacgtga ggcacgtcag cgctccgtca
      121 gtgactggat acgccagttg ctgccacccg gctggcagtt ccggcaacag aacgctgcca
      181 taccggcact gaacaaacgc ctccccgtct ggtccgttaa tgaccagtgg caccgggcac
      241 tgaatactct gctgaccggg cagcaactgt acggacatat ggactggaac aggaaaatac
      301 tgactgtcac gaccactgca gcaccgcctg ttgacctcac ggcaccgcag ggttcacaga
      361 aaacagcgga cacaccacgt aatccgttca ggagcacaac cggaacggct gcagaccagg
      421 cacaggtacc acctgccgtc agcgcttccc gggttcagag cacaacaggg acatccggta
      481 catctgcgcc ccgcacaacc acgccagccc cggtggtcac gaaaccggta gcaccaccac
      541 agaaggtcac accgccacgg cagtggaaaa tggaaaaagg gaccacgctg aaggacggac
      601 tggcccgctg gacgtcgtcc gaaaaatgcc cccacggcac ctggacgctg cgctgggaaa
      661 cgcccgttaa ctacagtatc gatgcccctc tggttttcac cggcagtttc cgccaggcgc
      721 tggaacagct cttcgcactc tacaccccgg tggaaaaacc gctgtatgcc cggaccagca
      781 cagcacaatg tctgctgcgg gtgacagatc gtccacagga ataaccttat gggctggttc
      841 atcgtgttca tcatggtcat cacgaccaca ggcaatttct ggttcaccag tcagctctcc
      901 cgcagcgaac accgtcatca ggccgcagaa aatacacaac aggcagcaac gttcattcgt
      961 tatatgaacg ccatcaatga ttacctgcat cagcatccgg aacgacggac agccggaggg
     1021 cggctgacat ccactcagct cgggattccc gggacggata ccgtcagtca tatcatcagc
     1081 cagcaacgtg tttttgtctg ggctacagaa accccgggcc tgatggccgc gctcagggaa
     1141 cagtgtaatg actctgctct gctcggcagg gtggaaaacg gccgactgct ggacactgcc
     1201 ggcagagcgc tttccgtcac acttccgtca gtgatccccg atcacgtcat cctctggatg
     1261 aattagtcac gaatctgaat aaatcacgga tctcccatga ataaaaaaat tcttgccatt
     1321 atgattgccg ccgctctgtt acagggctgt gccagttaca gacagcttga gagcacaaga
     1381 aaggaagccg ttgcggaaca acaacgggca cgggaaatga tgtctgatct gcgaacccgg
     1441 acgccggttg ttaaggaatc ctcacgacaa tggatcaatc cccgtccggt catggataaa
     1501 aacaccggca aaaccgttcc cggttgtccc attgtcattc atacccgtca gagcatcacc
     1561 cttcagcagg tggttcagcg gatcacggaa agctgccata ttccggtacg gatcacacct
     1621 gatgtactgg catacctgaa tacatccaca accggcagca cacaacaaat ccagggcgcg
     1681 ttacccccac cggatgcgag cggcatggtt ccccttgccg ccattggcgc atctgacacg
     1741 ccaccatccc gtctgccggg aaataccgca gcactgtctg aactgaatgc ctccggcctg
     1801 acgaccctgc tgaatgcggt ctcctcccgt ctcggtatat cctggcgcta tgacggctcg
     1861 catatcatct tctattatct cgagacacag accttcccca tcacctatat ggacagtcag
     1921 gtggcctata acgctactgt ggtcagcggc accatgtcct caaacggctc atcaggaggc
     1981 agtgcatcag atgccatttc cggtgatgcc agcaataccc agaccaccac agtggatatg
     2041 aagtctgcgc tgtatcagga tgtgaaaaat gcggtcacat caatgctgac cccgggtatc
     2101 ggacgcatgt tcatgtccac gggctttatc accgtcacag ataatccaca ggttctggaa
     2161 acggtccggg cattcattga aaaacgcaat gaagagatga aacgtcaggt ggtgctgaat
     2221 gtggaaattc tgagtatccg gaaaacccgc aatgagcagg ccggtatcga ctggaacgca
     2281 gtcttcagcg acagaacgct gggactgagt ctcggcagca cattcaccag tgcagccagt
     2341 gatgccatca caggtggtgt ttccattgtg gacggtaaac tcaccggttc aaaagccttc
     2401 ctgaaagcat tgtcttccca gggggatgtc agtgtggtga cccagaactc tgccgtcacc
     2461 aaaaacctga cgccggtgcc aatgcagatc gccaatcagc agagttacat agagagtgtg
     2521 acgacggata ccactgccaa tgtcggctca tccacctccc tgaatgcggc caccatcacc
     2581 accggtttta acatgaccct tctgccgttc attctcccgg actcacagac actgcagttg
     2641 ctctattcca tgagtctctc cgacaaaccg gttattgaga attatgagag cggtggaagt
     2701 aaagcccagc tgccgaatgt ggatctgaaa accatcaatc agaccgtgga tctgaaatcc
     2761 gggcagacgg tcattatcag tggcttccag cagtcaggac gtcgcagcgg aaaacagggt
     2821 gtaggcacgc cgggattctt cggtcttggc ggtgggatca acagtgaaaa cgacgacacc
     2881 attctcgtgg tgctgatcac gcccaatatc atctgatttg cacaaggagg acgatcgtga
     2941 cggatatttc accgacagag cggcagatgt ttccgctgac ggcagggcgc aatgtgtttc
     3001 tggccggtct ggaatggaaa acactgccct cacagtaccg gcatgcccgc gactttgccc
     3061 gggcacagaa agccgacctt ttcctggcct gtcagtatct cagtaatgaa gatgccaaca
     3121 cccacaccat ggtggccaca gccagtcgcc gcatactgcc cggaaaaccc cgtcagtacc
     3181 tctccctcgc cctgctgatc ctgccactgc ttgaacaggg cggctatgcc atcacggaac
     3241 tgacgttacc cggagaaaca ccgcgctaca gctttgtttc cgctgtcgac ggtgttctcg
     3301 tcagtgacct ggtgggaagc ggagaagagg tccgtgaagc ccgggatacc tttctttcca
     3361 tcaacaccga accggagcag ggctggacac gctatgaacc ggtggcattt tcagcaggcg
     3421 atcagaatca ggccctgccg ctgtccaccc tgaccggcgc tggtaaacat ccggctgccg
     3481 cgcgcctgaa tccggtatcc cggggagcgc agttcatcac tgtctcgctg atcatcgccc
     3541 ttctcggcgc tgcatggtac ggctggcagt attatgaacg gtggaagact gaacaagccg
     3601 cgcaggcagc agctgcccga caggtcacag aggaacaaat cagtcccccc tggcccggac
     3661 tccccgaaac cgacgcattt atccagggat gtgctgacat atggacgtca ctgccactgt
     3721 ctgccgccgg atggcgttat tcgctggctg aatgttcaac tgacggcggc agcggcaatc
     3781 ttcgcgcctc gtacactaat acagctggcg cgacagtgac tgacttcatc cggcgcatca
     3841 gtgatatgac gggcacccgc ccctttattg tgatgccgga aggaaagacc ggcggcattg
     3901 gcctgccagt cagcttccgc ctgcctgaac atcccgtcga cgttgatgct ctgccggaaa
     3961 gctctgtcct gcaggaacaa ctgaccacgc ttgctcagtc aatgccactg acgctggact
     4021 ggcaggaggt cagtaacagt gtcaccgatg aaaacggaaa tattatccag cctccgtgga
     4081 aagagtatga ccttcagata cagacgctac tcccggcata ccagctggtt gaacgtctga
     4141 aggcgccttc tgtccgtttt atctctgtca cacgacaact ggagaacggt cgtttccact
     4201 atcaatttac aggtaaatat tatgcacgct aaaacaaaag cccttctggt ttcagcgctg
     4261 ctgttaccgg caacggcaat ggccacaacc ggaaacgcag aggaacagag tccccggcaa
     4321 ctgcagattg agcagataca ggacgaaaca gccctgtatg aagccatgct ggcgagagag
     4381 aaaatccgtc atgagctgcg catgctggag cagggtattc ccgcagagcc cggtcgtccg
     4441 caggaaagca cgtccgccat cccgactgcc ccgtcgacgg cggcatcccc ccgtctgaca
     4501 gaaatatttg gtgtacagcc tcagctgagc gcccggatag aactttcggg tggacacatc
     4561 accgatgtga agacgggcca gaacatcccc ggaacccctt atctcgtgga gaaaatcaca
     4621 ccggacagtg tggtactgga gcagtccggc atcagaaaaa ccctgcgacc atgaaaccgg
     4681 ccgccagtac aggcggctgt ccgtacaaaa ggatgaacga caatgaacat cagtgaactg
     4741 atatcctggc tcagcctgat catccgtgac ctggaaacag ccgctgcaga atacggggtg
     4801 aaccacacag atattgtcca cgaggcaaca caattacagg aacaactttg tcggggaaaa
     4861 caggtcaccc cggcacaact ccgtgccctg agtgcccggt tgtggggtgc cagaatgcgc
     4921 ctcgcggcgc agtatggcca ggacgccccc ctcatgaacg atctggcgtt cctgagcaac
     4981 tgtctgaaat atgatgccga cagattaaac gaccgctggc gttaccggga atggatatcc
     5041 gcagccgaat cctttgtcct gccgctcgtc ttcattatcc cactgctgat cgtgctctgt
     5101 tatatgatga agagcggcaa ttccggaggc gctgaattat gcgcggcact tgccggcgca
     5161 tggtgcaccg gactgacatt cttatgcctg tgggcaaaag atcccgtcgg gcttttctgg
     5221 tcactctaca gcttcattcc gctgtatttc ctctggtgtg atatatcacc tgcgtaagcg
     5281 attacagtgc agaaataata ttccgtacaa tacatcacaa atcacctcac acccgggacg
     5341 ttttattatt aacactcgac gggatatatc tcatgctgga gaataaaacc accgatgatt
     5401 ttatttttgt taagcaacat atctaccgca tatttcagtg gatgcacgaa acacctctca
     5461 ttaacaacgg taatacaccg gtaactgaca ggttaatcgc cgatgtccgg tggatgctcg
     5521 aacatcatta ctatccctcc cccggccagg tggaccgtat tatatctgta ctgagggaga
     5581 caaaattaca gctgctccgt gaaaaaaccg acagacagga acttgtggaa gagatccagc
     5641 agaaactgaa cacgctgaaa gatatccgtc atttcctgaa gctgaacacg aactataaac
     5701 aggatggcga tatcatttca cttacaccat ctggtgaatg gaagaatact tccattattt
     5761 tatcggtagt tgccgtcatg cttatgatat tcgccagtat atgtaatctg tccggcatca
     5821 caccttattt tatttatacc gcaggtatta ttttttacgc tctcgccaca ccggtattaa
     5881 cgctgagatc aacacagccg gagaaaaaca ttcccctgtt attcgccttt ggaggcgtaa
     5941 tcatttttac actgttaagc ctgtgctgaa aatgtccaga ttctctccgc agaagaactg
     6001 attatttccc tcgcttaata acagaaaaca tattttccgg gcaccgctgc ccgtaaggag
     6061 gccttatgtt ggagtctgca gacctgccac cggatgacat tctggattat gtggctatcg
     6121 acacagataa aaccggtcat ctgcgggtca gggtcgtgga gggaaaaaaa catctgcgtg
     6181 ccgtccagga atatctgacc cgcctgcgcg cacgtcatca ggggcgtgtg ggagactttg
     6241 agttcaccac cctggatgtg atagcccgcc tcagacagga caccacaacc gccggtgatg
     6301 aaagcgtcat taacccggta cagcagaaaa tgctcggcta tatccgccat tctgcctctc
     6361 ttggtgcctc agaccttcat atgaccccgg gacgagacaa cacggacttc acctatgtgg
     6421 aagcccgggt tcacggcgaa ctggaagtgc tcgatatcct ccggaaagaa gaaggcctcg
     6481 aattactggg ggccacctat tccggcatga ccgatgttat caaaggcaca cagtttgatc
     6541 ccggggtgcc tcaggatgca cgtctttcag agcagtatct gaaactggcc ggtctgtttg
     6601 gtgcacgcta ctcccactac ccctgtgtgg gcggcctcta tgctgtgctc cgtctgatca
     6661 aggatgacag tcagcaaatc ccgaccttca gtatgctggg ctatcaccct gaccaggagc
     6721 gcactcttcg ccgcattctg cagcgccctg aaggcattat cacgctctca ggccccaccg
     6781 gctcaggaaa atccaccacg ctgagaaccg cctctgcggc gtaccttgaa cagtacggct
     6841 tcaacaacac cggcggtatt cttcttcccc gtcgcaggct gttcaccatc gaatccccgc
     6901 cggaagggcg tattcccggt gccattcaga ccgccgtcat ggataccgcg cagggctggg
     6961 tggactccat caaatcggca ctgcgtcttg accccgacgg gatcctgaac ggtgaaatcc
     7021 gcgaccacga ttccgccatt acggcaataa aagccgccat gaccggccat ctgatgctga
     7081 ccaccatcca tgcgaacgac ccgattaaca tcctcgaacg tcttgagatg gagggggtac
     7141 aggccaggat gattgccgac ccgcaactgt tcattggcct gctgagccag cgtctggttc
     7201 agctgatatg tccgcactgc cgtcgcccct ggcatgaggt ggcgaccgag cgcacggatg
     7261 atgaacggcg cctggtggaa agcgtctgca acccggacca ggtatatctg cgaaatcatg
     7321 aaggctgccc gcactgctgg cgtggcgtga caggacgaac ggtgatcgca gaagtcatct
     7381 ccccggatgc ccgttttttc cagttgtacc gggaaaaggg gcgtattgag gccagaacat
     7441 actggcaccg ggagctgggc ggcatgacac gaaatcagca tctgctggga aaaatcaaca
     7501 gcggacaggt tgatccgctg gcggcccatt acatctctcc catcgacgag gacagctata
     7561 cgctgctgca ctgacatgag atttctggaa aaactcaaca acaccgacat tgagctgaca
     7621 gcactgactg tctggatagc cagaaaaacc tttgccctga accgggtgga gttctacgaa
     7681 gccctgggcg gcatgatacg tgacggaacg ccgtccctgc gtgcactcga atttctctgc
     7741 gagatcgaaa ccgattttgg tgaaaagaaa ggcacatccg gcatgtattt tctggcaaaa
     7801 gaatgcatcg attccatcaa agccagcggc atgttatccg gtgccctgtt aacctgggtg
     7861 ccaccggagg aagccacgtt aatcagaaac ggggaagagc gcggaaatat tgccgacgcc
     7921 cttttccagg tggccagaac cgttaaagcc cggcgggaaa tgacatccag cctcattgcc
     7981 gtctgcctct acccccttct gctgctttcg ctctgtgtgg tgaatatgta caacgcccat
     8041 acccgtatca tccccatcgt cagtgcgttt gcttcagaag ataaatggtc attccagatg
     8101 aaattgcttg ccggcatgtc ggaatttttc atcgggtacg gattctggct ctgtgggacc
     8161 attttcgccc tgacgcttct gatccgtttt tcattcagcc ggtataccgg ccccggccgg
     8221 aggatgctgg acaaggctcc cccctggtca ctgtatcaga ccttacacgg ggtgaattat
     8281 ctgttcagta tttcatccat gctgcagatt aatattcctc tttcgcaggc gctgctccga
     8341 atggaaaaag gtgcagaaaa gaataaatgg ttaaaggaga gaattcaggc aatacgaaaa
     8401 catgtattat ccggtcagaa tctggccaca gcaatgcgaa attccggtta tgattttccg
     8461 tccagaatat gcatcaataa actgctcctg aactccgaaa gggaggacag tgtggcaacc
     8521 atggcactct atgccgatca gtggctggag gaagcaaaaa aacgggtaaa acgaattgga
     8581 ttaattatta ccggtatatc aggaggtttt gtcttctttt ttattcttaa catgatatct
     8641 gccatctaca gtatcagcga catgctgaaa caataatc
//
LOCUS       DAZFLD010000191         8633 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.192, whole genome shotgun sequence.
ACCESSION   DAZFLD010000191 DAZFLD010000000
VERSION     DAZFLD010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8633
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.192"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            49..789
                     /locus_tag="ACHKBL_003933"
     CDS             49..789
                     /locus_tag="ACHKBL_003933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HFO5820734.1"
                     /translation="MFFNTKHTAALCLASCMAFSSSAIADIVISGTRVVYKSEQKSVN
                     VRLENKGNNPLLVQSWLDTGDDNAEPGSITVPFTATPPVSRIDAKRGQTIKLMYTASS
                     ALPKDRESVFWFNVLEVPPKPDAAKAANQSLLQLAFRTRIKLFYRPEGLSGIPSDAPL
                     ALKWSWATSEGKAALRVDNPTPYYVSFSSGDLEANGKRYPLDVKMIAPFSNDVIKVNG
                     MSGRTSSAKVHFFAINDFGGSIEGNANL"
     gene            824..3424
                     /locus_tag="ACHKBL_003934"
     CDS             824..3424
                     /locus_tag="ACHKBL_003934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane usher protein"
                     /protein_id="HFO5820735.1"
                     /translation="MTIKSTKKYHNHLTRIATFCALLYSNSALCAELVEYDHTFLMGK
                     DASNIDLSRYTEGNPTLPGIYDVSVYVNDQPIMSQSIAFTVIEGKKNAQACITQKNLL
                     QFHISSPDKNSEKAILLKRDEDLGDCLNLAEMIPQSSIRYDVNDQRLDIDVPQAWIMK
                     NYQNYVDPSLWENGINAAMLSYNLNGYHSESPGRTNDSIYAAFNGGINLGAWRLRASG
                     NYNWMTNVHSDYDFQNRYLQRDLASLRSQLVIGESYTTGETFDSVSIRGIRLYSDSRM
                     LPPVLASFAPIIHGVANTNAKVTVMQNGYKIYETTVPPGAFAIDDLSPSGYGSDLIVT
                     IEEADGTKRTFSQPFSSVVQMLRPGVGRWDISAGQVLKDSIQDEPNLFQASYYYGLNN
                     YLTGYTGIQLTDNNYTAGLLGLGMNTPVGAFSVDVTHSNVSIPDDKTYQGQSYRISWN
                     KLFENTSTSLNIAAYRYSTQHYLGLNDALTLIDEVEHPEQDLEPKSMRNYSRMKNQVT
                     VSINQPLKFEKKDYGSFYLSGSWSDYWASGQNSTNYSIGYSNSASWGSYSISAQRSLN
                     EDGQTDDSIYLSFTIPIENLLGTEHRSSGFQSIDTQLNSDFKGNNQLNISSSGYSDTN
                     RISYSVNTGYMMNKSSDDLSYIGGYASYESPWGTLSGSASASSDNSRQFSLNTDGGFV
                     LHSGGLTFSNDSFSDSDTLAVIQAPGAKGARINYGNSTVDRWGYGVTSALSPYHENRI
                     ALDINDLENDVELKSTSTVAVPRQGAVVFADFETVQGQSAIMNIVRSDGKNIPFAADI
                     YDEQNNIIGNVGQGGQAFVRGIGQEGNIRITWIEEGKPVSCFAHYQQNTTSEKIAQSI
                     ILNGLRCQIQ"
     gene            3441..4013
                     /locus_tag="ACHKBL_003935"
     CDS             3441..4013
                     /locus_tag="ACHKBL_003935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO5820736.1"
                     /translation="MIKTTPHKIAILMGLLLSPSVFATDVNVDFTATVKATTCNITLT
                     SLNGSNVVDNGNDSYTLIIPKMGLDKIVNKASQSEANFKLVASGCSSGISWIDTTMTG
                     NQSGSTSLIIPQSSDSSSTTSNIGMGFKRLETSGDTFLKPNSAEYIRWKTTEISTSGL
                     EMTVALRETTPGQGIPGKFRAQATFNFIYQ"
     gene            4025..4633
                     /gene="yadL"
                     /locus_tag="ACHKBL_003936"
     CDS             4025..4633
                     /gene="yadL"
                     /locus_tag="ACHKBL_003936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020235143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein YadL"
                     /protein_id="HFO5820737.1"
                     /translation="MMILKKLCYGLSGSLFLASSLLTISSHAATDSIGLTVTTDIEMG
                     TCTSTLTDDGTAKLSTINFGDVYISEINAETKIKTFKLQFKDCAGIPGKKAKIKLTPR
                     ALCEGNSNNGPGFANASTATAKAAAVAVEVWSTSTPGKNGAKQFSCVTPATEEVSIAG
                     ATGGDVVDYPMSAVLVVAKDKTVTDVTAGDFTAPATFTVTYN"
     gene            4660..5256
                     /locus_tag="ACHKBL_003937"
     CDS             4660..5256
                     /locus_tag="ACHKBL_003937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein"
                     /protein_id="HFO5820738.1"
                     /translation="MRPTQRNLMKKSILLLSLLFSTTFPVVAGQDVDIVANIKNNTCQ
                     SGISNNGNIDLGVVGVGDFTGNISAENYHPGGKEFTITVQDCTLQGTGNVLNQLHIDF
                     RALSGVMATGSSQIFANEDNTGAKNVGVVIFSIQDPANTFNVLGVTGSSRSVYPVMSS
                     ALNNSSWKFSTRMQKIDPALNVTSGQLISHVLVDIYYE"
     gene            5282..6571
                     /locus_tag="ACHKBL_003938"
     CDS             5282..6571
                     /locus_tag="ACHKBL_003938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000846622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like adhesin"
                     /protein_id="HFO5820739.1"
                     /translation="MQHDNGVKMKIFFRYFLFLALCSCCYTASAGTDDNVSYLVGNDY
                     GVGPGDQKWRETGPSADITVKFRYSTSTNNLVFYKPTQLGPTGVSLYWSQLNSASGGG
                     YLYCNRSNTASGEPMAIENQMVDSGKRYGGHKLFETSVPGLYYTLKISNIWSSYTTTN
                     ISSSGMYIGDSPTQYFRWIPESEATLYWSCNNANSSNKYWAVGGVWQSLTVEFYTDTN
                     FDPTKTNKVTLSKPGGYLYSFKGYGLGGGINDHSYFLKIDFDLDNMTLSNPTCFTAAL
                     TGPSVSGSTVKMGDYTPAQIKNGATAVPFDITLQNCIRVRNIETKLMSNKVGSVSKEL
                     LANTLTGNNAAKGVGVLIEGLKNTKSAQMVLKPNDSTSVYKDYETEDDTTGGIYPDKG
                     NGTSQPLHFQATLKQDGNIAIEPGDFKATSTFQVTYP"
     gene            6683..7477
                     /gene="panB"
                     /locus_tag="ACHKBL_003939"
     CDS             6683..7477
                     /gene="panB"
                     /locus_tag="ACHKBL_003939"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HFO5820740.1"
                     /translation="MKPTTIASLQKCKQDKKRFATITAYDYSFAKLFAEEGLNVMLVG
                     DSLGMTVQGHDSTLPVTVADIAYHTAAVRRGAPNCLLLADLPFMAYATPEQAFENAAT
                     VMRAGANMVKIEGGEWLVETVQMLTERAVPVCGHLGLTPQSVNIFGGYKVQGRGDEAG
                     DQLLSDALALEAAGAQLLVLECVPVELAKRITEALAIPVIGIGAGNVTDGQILVMHDA
                     FGITGGHIPKFAKNFLAETGDIRAAVRQYMAEVESGVYPGEEHSFH"
     gene            7489..8340
                     /gene="panC"
                     /locus_tag="ACHKBL_003940"
     CDS             7489..8340
                     /gene="panC"
                     /locus_tag="ACHKBL_003940"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HFO5820741.1"
                     /translation="MLIIETLPLLRQQIRRLRMEGKRVALVPTMGNLHDGHMKLVDEA
                     KARADVVVVSIFVNPMQFDRPEDLARYPRTLQEDCEKLNKRKVDLVFAPSVKEIYPNG
                     TETHTYVDVPGLSTMLEGASRPGHFRGVSTIVSKLFNLVQPDIACFGEKDFQQLALIR
                     KMVADMGFDIEIVGVPIMRAKDGLALSSRNGYLTAEQRKIAPGLYKVLSSIADKLQAG
                     ERDLDEIITIAGQELNEKGFRADDIQIRDADTLLEVSENSKRAVILVAAWLGDARLID
                     NKIVELA"
     gene            complement(8422..8631)
                     /locus_tag="ACHKBL_003941"
     CDS             complement(8422..8631)
                     /locus_tag="ACHKBL_003941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000404120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFO5820742.1"
                     /translation="MKTRRETRQTLAQWFEEKGFQKGFQKGYKEGLQKVRLAQRLLSK
                     GMSREDVAEVTALSLAEIDKLINSN"
BASE COUNT         2489 a         1981 c         1961 g         2202 t
ORIGIN      
        1 tttttgaacg ttattccctg ttttaattaa ctctgtgagg aacggtttat gttttttaat
       61 actaaacata ccgcagcatt gtgcttagcg agctgtatgg cttttagctc atcagctatt
      121 gcagacatcg taatttcagg tacacgcgta gtttataaaa gcgaacaaaa aagcgtcaat
      181 gtgcgtctgg aaaataaagg caataaccca ttgctggttc agagttggtt agatactgga
      241 gatgacaacg ctgaaccagg cagtattact gtccccttta ccgctacgcc accagtatca
      301 cgtattgatg ctaaacgtgg ccaaacaatc aaactgatgt acaccgccag tagtgctctg
      361 ccaaaagaca gagaaagtgt gttctggttt aacgtactgg aagttccacc aaaacccgat
      421 gcagcaaaag cagcaaatca aagcctgcta caactggcat ttcgtacacg tataaaactg
      481 ttctatcgcc cggaaggatt gagtggaatt ccatctgatg cgccattggc tctgaagtgg
      541 tcctgggcaa catctgaagg caaagcggca ttacgcgttg ataaccctac gccctactat
      601 gtctctttta gcagtggtga tttagaagcc aacggaaaac gctatcccct ggatgtgaaa
      661 atgattgcac cgttcagtaa cgacgtcatc aaggtaaatg ggatgagtgg cagaacaagc
      721 tctgcaaaag tgcatttttt cgccattaat gattttggcg gctctattga aggcaacgcc
      781 aacctttaat ctggcaagga tataccacag caggaagcag aacgtgacta ttaaatccac
      841 taaaaaatat cataatcacc ttacccgcat cgccacgttt tgcgcgctgc tgtatagcaa
      901 cagtgcttta tgcgctgaac tcgtcgaata tgaccataca tttctgatgg gaaaggatgc
      961 ttcaaatatc gaccttagtc ggtatacaga aggcaacccg acactaccag gcatttacga
     1021 tgtcagtgtg tatgttaacg atcaacctat catgagccaa agcattgctt ttactgttat
     1081 tgaaggtaaa aagaacgctc aggcctgcat tacgcagaaa aatttattgc aatttcatat
     1141 cagttcacca gacaagaata gcgaaaaagc cattttgctt aagcgagacg aagatcttgg
     1201 tgattgcctt aatctggctg aaatgatccc tcaatcatcc attcgttacg atgttaacga
     1261 tcaacgcctg gatatcgacg tcccccaggc atggattatg aagaattatc aaaactatgt
     1321 tgatccctct ttgtgggaaa atggcattaa tgctgccatg ctttcctaca acctcaacgg
     1381 atatcacagt gaaagtccag gcagaacaaa tgacagtatc tacgcggcat tcaacggtgg
     1441 gataaactta ggtgcctggc ggttacgtgc ctcgggtaac tacaactgga tgaccaatgt
     1501 ccatagcgat tacgattttc agaatcgcta tttacaacgc gatcttgcgt cactacgttc
     1561 acaactggtt attggtgaat cttacacaac cggcgaaacc ttcgattcag tcagtattcg
     1621 cggtattcgc ttatacagtg acagtcgaat gctgcctccg gtattagcca gttttgcgcc
     1681 aatcattcat ggcgtagcga ataccaatgc caaagtaacc gttatgcaaa atggttataa
     1741 aatctatgaa acgacggtgc cgccaggggc ctttgccatt gatgatctta gcccgtcagg
     1801 ttatggcagt gatctcattg tgactatcga agaagctgat ggcacaaaac ggacgttctc
     1861 ccagccattt tcatctgttg tgcaaatgct gcgccctggc gttggtcgct gggatattag
     1921 cgccggtcag gttttaaaag acagtatcca ggatgagcct aatttatttc aagccagcta
     1981 ctactatggt ttgaataact acctgacagg atataccggt attcagctaa ctgataataa
     2041 ctataccgcc ggattgctgg ggcttggtat gaataccccc gtgggggctt tttctgtcga
     2101 tgttactcac tcaaatgtca gcattccgga cgataaaacc tatcaaggac aaagctaccg
     2161 tatttcctgg aacaagttat ttgaaaatac cagtacatca ttaaatatcg cggcctatcg
     2221 ctactccacc cagcattatc tgggcctcaa tgatgcgctg acgctgattg atgaagtaga
     2281 acatcctgag caagatcttg agcctaaatc catgcgcaat tactcgcgca tgaaaaatca
     2341 ggtcacggtc agtatcaacc aaccgctgaa atttgagaaa aaggattacg gttcatttta
     2401 tctctcggga agctggtcag attactgggc ctccgggcaa aacagtacta attactctat
     2461 tggttatagc aatagtgcgt cctggggcag ttacagtatc agtgctcaac gttcgttgaa
     2521 tgaagatggt caaaccgacg atagcattta tctcagcttc acgattccaa tagaaaattt
     2581 actgggtacg gaacaccgca gctccggctt ccagagtatt gatacacaat taaacagcga
     2641 ctttaagggt aataaccaac tcaatatcag cagtagtggt tacagcgata ccaatcgcat
     2701 cagctacagc gtaaacacgg gttatatgat gaataaatcc agtgatgacc tgagttatat
     2761 cggcggttat gccagttatg aatcgccgtg gggaactctg tcaggttcag cctcagcaag
     2821 cagtgataat agtcgccaat tctcactcaa tactgatggt ggatttgtgt tacatagcgg
     2881 cgggctgacg ttcagtaatg acagttttag tgattccgac actctggctg tcattcaggc
     2941 gccaggagca aaaggcgctc gtattaacta cggaaacagc accgtagacc gctggggtta
     3001 tggtgtgacc agcgcccttt cgccatacca tgaaaaccgt atcgcactgg acattaacga
     3061 tctggaaaac gatgttgaat tgaaaagcac cagtactgtc gccgtcccgc gccagggagc
     3121 cgtcgtcttt gctgattttg aaacagttca gggacaatcg gcaattatga atatcgtgcg
     3181 aagcgatggt aaaaatattc catttgcagc ggatatttat gatgaacaaa acaatatcat
     3241 cggcaacgta ggtcagggtg gtcaggcctt tgttcgcggt atcgggcagg aaggaaatat
     3301 acgaattacc tggattgaag agggcaaacc agtcagttgt tttgctcatt accaacaaaa
     3361 cacgacatca gaaaaaatag cgcaatctat tattctgaac ggacttcgtt gtcagataca
     3421 gtaaccacaa gggacttcca atgataaaaa ccacaccaca taaaatagca atactcatgg
     3481 ggctattatt atcgccatct gtatttgcaa cggatgttaa tgtcgatttt accgctaccg
     3541 tgaaagcgac aacatgtaac atcaccctga catcgctgaa tggttcaaac gtcgttgata
     3601 atggcaatga tagttatacc ctgataatcc ccaagatggg actggataag atagtcaata
     3661 aagcgtcaca atctgaggcc aacttcaaat tagtcgccag tgggtgtagc agtggaatta
     3721 gctggattga taccacaatg actggaaatc agtctggtag tacatcgctg attattccac
     3781 aatcttctga ctcctcctca acaaccagta atattggtat gggatttaaa agattagaga
     3841 cttcgggcga tacgtttctg aaacccaata gcgcggaata catccgctgg aaaaccacag
     3901 aaattagcac cagtggtctg gaaatgactg tcgcactgcg cgaaacaacc ccaggacagg
     3961 gcatacccgg gaaatttcgc gcacaggcaa cgttcaattt catctatcaa taacaaggat
     4021 gacaatgatg atattaaaaa aattatgtta tggattatcg ggtagtctat ttctggcttc
     4081 atcactgtta accatttcct cacatgcggc cactgatagt attggcctga ctgttactac
     4141 agacatagaa atgggtacct gtacatcaac gttgactgat gacggtacag ctaaactgtc
     4201 caccatcaat ttcggtgatg tatatatttc tgaaattaat gccgaaacta aaattaaaac
     4261 cttcaaattg cagttcaaag actgtgcggg gattcccggg aaaaaggcca aaataaagtt
     4321 gaccccacga gccttatgcg aaggtaattc taataacggc cctggatttg ctaacgcatc
     4381 aacggcaaca gcaaaagcag cggcagtcgc cgttgaggtc tggagtacgt ccacaccagg
     4441 taaaaacggc gcaaaacaat tcagttgcgt cacccccgca acagaagaag tcagtattgc
     4501 tggtgcgacg ggaggtgatg ttgtcgatta cccgatgagt gctgttttgg tggtcgcgaa
     4561 agataaaacc gttacagatg tcaccgcggg tgatttcacc gctccggcaa catttactgt
     4621 gacttataac taacaggcag accgattcag gaactaaata tgcgccccac tcaacgtaat
     4681 ctgatgaaaa aatcaattct gcttctgtct ttgctattca gcaccacttt ccctgtggtt
     4741 gcaggacagg atgttgacat tgttgcaaat ataaaaaata acacctgcca aagtggaatc
     4801 agcaacaacg gaaatattga tttaggtgtc gttggcgtgg gcgattttac cggaaacatt
     4861 tcagccgaaa attatcaccc cggtggaaaa gagttcacta ttacagtaca ggactgcaca
     4921 cttcagggca cagggaatgt gttaaatcag ttacatattg attttcgcgc actcagtggc
     4981 gtaatggcga caggttccag tcaaatattc gccaatgaag acaacaccgg ggctaaaaat
     5041 gttggggtag ttatattctc tattcaggac ccggcaaata ccttcaacgt tcttggcgta
     5101 actggtagct cacgttccgt ttatccggtc atgtcatcag cactaaacaa ttcatcctgg
     5161 aaatttagca ctcgaatgca aaaaatcgat ccagcgctga atgtcacctc tggccaactt
     5221 ataagccatg tgttagtgga tatttactac gaataacaga caaagtgctg caagcattat
     5281 cttgcagcat gacaatggag taaaaatgaa gatattcttc agatactttc tttttttagc
     5341 actatgctct tgttgttata cagcaagtgc cggcaccgat gataacgtca gctatcttgt
     5401 tgggaatgat tacggagtgg gtccgggaga ccaaaagtgg agagaaacag gccccagtgc
     5461 agacattact gtcaaattca ggtattcaac aagcacaaat aacttagttt tttataaacc
     5521 aacccaactc ggaccaacag gtgtgtcatt atactggtct cagttaaata gcgcttcagg
     5581 tggaggttac ctttactgca acagaagtaa cactgcaagc ggtgagccta tggcaattga
     5641 aaatcaaatg gttgattcgg gtaaacgtta tggcgggcat aaactatttg aaacatctgt
     5701 cccaggcctg tattatacat taaagatatc aaacatttgg tcatcataca ccacaaccaa
     5761 tatcagttca tcaggtatgt atatcggcga ttctccaact caatattttc ggtggatccc
     5821 cgaaagtgaa gccactctat attggtcttg taacaatgca aactcatcta ataaatactg
     5881 ggccgtaggt ggagtttggc agtcgctcac ggtggaattt tataccgata ccaactttga
     5941 ccccacaaag acaaacaagg taacattatc taagccgggt ggttatctct actcattcaa
     6001 aggatatggt cttggtggcg gtatcaacga tcacagctat ttccttaaaa tagattttga
     6061 cctggataat atgacgttat ccaacccaac gtgttttact gctgcactta ctggcccatc
     6121 tgtaagcggt tcaacagtta aaatgggaga ttatacccca gcgcaaataa aaaatggcgc
     6181 aacagcggtt ccttttgata tcacgctaca gaactgtatt cgtgttcgca atatagaaac
     6241 aaaacttatg tcaaacaagg tgggcagtgt aagcaaagaa cttctggcta acacactcac
     6301 aggaaataac gcagcaaaag gtgttggtgt actgattgaa ggtttaaaaa acactaaaag
     6361 tgcacaaatg gtactgaaac caaatgattc aacgtctgtt tataaagatt acgaaacaga
     6421 agatgacact accggaggaa tttacccgga taaaggaaac ggtacctcac aacctcttca
     6481 tttccaggct acattaaaac aagacggtaa tattgctata gaaccggggg attttaaagc
     6541 caccagcact tttcaggtta cctacccctg ataacactat tgcaccgcca gacaatggcg
     6601 gtgcgatacg gataaatcat tgcccccacc aatgtgactg ataaaatgcg agacagcaca
     6661 acatcattta tcaggatacg ctatgaaacc aaccacgatt gcttcattgc agaaatgcaa
     6721 gcaggacaaa aaacgtttcg caaccatcac cgcttacgac tatagcttcg caaaattgtt
     6781 tgccgaagaa ggacttaacg tcatgctggt aggcgattca ctgggcatga cggttcaggg
     6841 gcatgactcc acgctaccgg ttaccgttgc cgatatcgcc tatcacaccg ccgctgtgcg
     6901 tcgcggcgcg ccaaactgcc ttctgctggc tgacctgcca tttatggcgt atgccacgcc
     6961 ggaacaagcc tttgaaaacg ccgcaacggt aatgcgtgcc ggagccaaca tggtcaaaat
     7021 tgaaggcggt gagtggctgg tggaaaccgt acaaatgctg accgaacgtg ccgttccagt
     7081 ttgtggtcat ttaggcctaa ccccacagtc agtgaatatt ttcggtggct acaaagtcca
     7141 ggggcgcggc gatgaagcgg gcgatcaact gctcagcgat gcattggcat tagaagctgc
     7201 tggggcacag ctgctggtgc tggaatgcgt gccggttgaa ctggcaaaac gtattaccga
     7261 agcactggcg atcccggtta ttggcattgg cgcaggcaac gtcactgacg ggcagatcct
     7321 cgtgatgcac gacgcgttcg gcattaccgg cggccacatt cctaaattcg ctaaaaattt
     7381 cctcgccgaa acgggcgaca tccgcgcggc tgtgcggcag tatatggctg aagtggagtc
     7441 cggcgtttac ccgggcgaag aacacagttt ccattaagga gtgacgttgt gttaattatc
     7501 gaaaccctgc cgctgctgcg tcagcaaatt cgccgcctgc gtatggaagg taagcgcgtg
     7561 gcgctggtgc ccaccatggg taacctgcac gatggccata tgaagctggt cgacgaagcc
     7621 aaagcccgcg ccgatgtggt agtggtcagt attttcgtta acccgatgca gtttgaccgc
     7681 ccggaagatc tggcacgtta tccacggacc ttgcaagagg actgcgaaaa gctaaacaaa
     7741 cgtaaagtgg atttagtttt cgccccttcg gtaaaagaga tctacccgaa cggtacagaa
     7801 acccacacct atgttgatgt tcctggtctt tccaccatgc tggaaggtgc cagccgtccg
     7861 ggacattttc gcggcgtttc gacgattgtc agcaagctgt tcaacctggt ccagccggac
     7921 atcgcctgct tcggtgaaaa agattttcag caactggcgc tgatccgcaa aatggttgct
     7981 gatatgggct ttgatattga gattgtcggt gtgccaatta tgcgcgccaa agacggtctg
     8041 gcgctgagtt cccgtaacgg ttatctgacg gcggaacaac gcaaaattgc gcccggtctg
     8101 tacaaagttt taagttcgat tgccgacaaa ttgcaggctg gcgaacggga tctcgatgaa
     8161 attattacta ttgccgggca agaactgaat gaaaaaggct tccgcgccga tgatattcag
     8221 attcgtgatg ccgacacctt gctggaggtc tctgaaaaca gcaaacgtgc agtgattctg
     8281 gtagccgcct ggcttggcga tgcccgcctg atcgacaaca aaatcgtcga actggcgtaa
     8341 tccttatctg gcgctacggc tgatggcgcc agttattcat tgccacgtta tctgtaggca
     8401 attattcata tcgtagacaa cttagttaga gttaattagc ttatcaatct ctgcaagaga
     8461 taaagctgtc acctctgcaa cgtcttcccg agacattcct ttactcagaa gacgctgggc
     8521 cagtcttacc ttctggagtc cctctttata tcccttctga aatcccttct gaaatccctt
     8581 ctcttcaaac cactgcgcca acgtctgcct ggtttctctc cgtgtcttta ttt
//
LOCUS       DAZFLD010000192         8509 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.193, whole genome shotgun sequence.
ACCESSION   DAZFLD010000192 DAZFLD010000000
VERSION     DAZFLD010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8509
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.193"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            25..1245
                     /locus_tag="ACHKBL_003942"
     CDS             25..1245
                     /locus_tag="ACHKBL_003942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid deaminase"
                     /protein_id="HFO5820743.1"
                     /translation="MKYHSEPLVPHKSALMQMPANLLAEDVCLPAAIIKKQALENNIT
                     WMQRYADARGVSLAPHGKTTMTPWIFQAQQAAGAWAIGVGSAWQAGAAMASGIQRVLM
                     VNQLVGKANMQLIAQLQRHYPTVDFICCIDSIENARALSAFFASQQQTLNVMIELGVP
                     GGRCGCRSRDAALTLAKQAAQLPGLRLRGLELYEGVLHGDDPQPQVEALLRDAAQLAC
                     DMASLVDGEFILTGAGTVWYDVVCNIWLAAEKPDNCRIVIRPGCYITHDTGIYDAAQQ
                     QLLARDPVACDLAGDLTSALELVAMVQSVPEADRAVVNFGKRDCAFDAGLPQPVAHYR
                     NGKSLAFDPQAIRSTGIMDQHCMLQLSANSDVQVGDILVFGTSHPCLTFDKWKTLLLT
                     DDDYNVLAELDTLF"
     gene            1513..3663
                     /gene="btsT"
                     /locus_tag="ACHKBL_003943"
     CDS             1513..3663
                     /gene="btsT"
                     /locus_tag="ACHKBL_003943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter BtsT"
                     /protein_id="HFO5820744.1"
                     /translation="MDTKKIFKHIPWVILGIIGAFCLAVVALRRGEHVSALWIVVASV
                     SVYLVAYRYYSLYIAQKVMKLDPTRATPAVINNDGLNYVPTNRYVLFGHHFAAIAGAG
                     PLVGPVLAAQMGYLPGTLWLLAGVVLAGAVQDFMVLFISSRRNGASLGEMIKEEMGPV
                     PGTIALFGCFLIMIIILAVLALIVVKALAESPWGVFTVCSTVPIALFMGIYMRFIRPG
                     RVGEVSVIGIVLLVASIYFGGVIAHDPYWGPALTFKDTTITFALIGYAFVSALLPVWL
                     ILAPRDYLATFLKIGVIVGLALGIVVLNPELKMPAMTQYIDGTGPLWKGALFPFLFIT
                     IACGAVSGFHALISSGTTPKLLANETDARFIGYGAMLMESFVAIMALVAASIIEPGLY
                     FAMNTPPAGLGITMPNLHEMGGENAPIIMAQLKDVTAHAAATVSSWGFVISPEQILQT
                     AKDIGEPSVLNRAGGAPTLAVGIAHVFHKVLPMADMGFWYHFGILFEALFILTALDAG
                     TRSGRFMLQDLLGNFIPFLKKTDSLVAGIIGTAGCVGLWGYLLYQGVVDPLGGVKSLW
                     PLFGISNQMLAAVALVLGTVVLIKMKRTQYIWVTVVPAVWLLICTTWALGLKLFSTNP
                     QMEGFFYMASQYKEKIANGTDLTAQQIANMNHIVVNNYTNAGLSILFLIVVYSIIFYG
                     FKTWLAVRNSDKRTDKETPYVPIPEGGVKISSHH"
     gene            3713..3916
                     /gene="yjiX"
                     /locus_tag="ACHKBL_003944"
     CDS             3713..3916
                     /gene="yjiX"
                     /locus_tag="ACHKBL_003944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HFO5820745.1"
                     /translation="MFGNLGQAKKYLGQAAKMLIGIPDYDNYVEHMKTNHPDKPYMSY
                     EEFFRERQNARYGGDGKGGMRCC"
     gene            3927..4883
                     /gene="yjiA"
                     /locus_tag="ACHKBL_003945"
     CDS             3927..4883
                     /gene="yjiA"
                     /locus_tag="ACHKBL_003945"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HFO5820746.1"
                     /translation="MNPIAVTLLTGFLGAGKTTLLRHILNEQHGYKIAVIENEFGEVS
                     VDDQLIGDRATQIKTLTNGCICCSRSNELEDALLDLLDNLDKGNIQFDRLVIECTGMA
                     DPGPIIQTFFSHEILCQRYLLDGVIALVDAVHADEQMNQFTIAQSQVGYADRILLTKT
                     DVAGEVEKLRERLARINARAPVYTVTHGDIDLGLLFNTNGFMLEENVVSTKPRFHFIA
                     DKQNDISSIVVELDYPVDISEVSRVMENLLLESADKLLRYKGMLWIDGEPSRLLFQGV
                     QRLYSADWDRPWGDETPHSTMVFIGIQLPEDKIRAAFAGLRK"
     gene            complement(4917..5219)
                     /locus_tag="ACHKBL_003946"
     CDS             complement(4917..5219)
                     /locus_tag="ACHKBL_003946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004020443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="HFO5820747.1"
                     /translation="MSMVKHKRGTSSTLDVQHEAELKALANKSDEDIDYSDIPPSSDE
                     QWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKGYQTRLNAILREAMLRDQNKK"
     gene            complement(5234..5335)
                     /locus_tag="ACHKBL_003947"
                     /pseudo
     CDS             complement(5234..5335)
                     /locus_tag="ACHKBL_003947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463387.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
     gene            5532..6698
                     /locus_tag="ACHKBL_003948"
     CDS             5532..6698
                     /locus_tag="ACHKBL_003948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000800831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HFO5820748.1"
                     /translation="MKNYKVITPLFPTYAQVKAMMKAVSGYSLKAVRNMITAIHEQTG
                     TPQKPVDWSEPDLWISERLTGEDADIARRIWDTDNHILNPRHSYGCYLFLNYPQFELM
                     ESTPDDTWQPTSRGQKFLQDDEKTLRSLDDQEGILQLLELLAGREMSRRADLLPEWQA
                     FLHQHSKFASASSVKSTLYSRLYNLIDRDMVDREGMSYRITDTGRAWLGKALPARKSD
                     PRKELLEAVKRYNEQQKELLREQISTMNPYKFEQLVGQLLEAMGYEQVEVTKASGDKG
                     VDVIGQVQVGITTITEVVQVKRMQNTITRPYIDQLRGALPYHKAIRGTLITTGKFAAK
                     CAEAALFPGAAPITLIDGDRLLELLIENNVGIRRSNAVELLDVDLQLFDELEMD"
     gene            6762..8381
                     /locus_tag="ACHKBL_003949"
     CDS             6762..8381
                     /locus_tag="ACHKBL_003949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003845607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HFO5820749.1"
                     /translation="MAKAPTKKAKAKKGFEETLWDTANQLRGSVESSEYKHVVLSLVF
                     LKFISDKFEARRKKMIADGQADFLEMEVFYQQDNIFYLPEEARWSFIKQNAKQDDIAV
                     RIDTALSTIEKRNPTLKGALPDNYFSRQNLETKKLASLIDTIDNIETLAHETDVETLS
                     KEDLVGRVYEYFLGKFAATEGKGGGEFYTPKCVVTLLTEMLEPFQGKIYDPCCGSAGM
                     FVQSVKFVESHQGKSRDIALYGQELTATTYKLAKMNLAIRGLSANLGERPADTFFSDQ
                     HPDLKADYILANPPFNLKDWRNEAELTKDPRFAGYRMPPTGNANYGWILHMLSKLSAN
                     GTAGFVLANGSMSSNTSGEGEIRAQMIENDLIDCMIALPGQLFYTTQIPVCLWFMTKS
                     KAADPAKGYRDRQGETLFIDARNLGTMISRTTKELTAEDIATIADTYHAWRSTPEELA
                     ARIARGDSKLEKYEDQAGFCKVATLQDIKDNDYVLTPGRYVGAAEQEEDGVAFETKMR
                     ELSKTLFEQMKQAEELDRAIRQNLEVLGYGE"
BASE COUNT         2050 a         2211 c         2236 g         2012 t
ORIGIN      
        1 tgaaaaactt aaaaggtaaa tgttatgaaa taccactcag aacctctggt tccccataaa
       61 tctgccctga tgcaaatgcc cgcaaatctc cttgccgaag atgtctgttt acctgccgcg
      121 atcattaaaa agcaggccct ggagaacaac attacgtgga tgcaacgcta cgctgacgcg
      181 cgcggcgttt cactggcacc gcacggtaaa accaccatga cgccgtggat ttttcaggcg
      241 cagcaggcgg ctggtgcctg ggcaataggt gtcggtagcg catggcaggc tggtgcggca
      301 atggcgagcg gtattcagcg agtgctgatg gttaaccagc tggtcggcaa ggcgaatatg
      361 cagttgattg cccagttgca acgacattac ccgacggtcg attttatctg ctgtatcgac
      421 agcattgaga acgcccgcgc tttgtctgcc ttttttgcca gtcagcaaca gacactgaac
      481 gtgatgattg agttgggtgt ccccggcggg cgctgtggtt gccgctcaag ggatgccgca
      541 ctgacactgg ctaaacaggc agcccaatta ccggggttac gcctgcgggg gcttgagtta
      601 tatgaaggcg ttttacacgg tgacgatcca caaccgcagg tagaagctct gctgcgggat
      661 gccgcacaac tggcctgtga tatggcaagt ctggttgatg gcgagtttat tctcaccgga
      721 gcaggcacgg tctggtatga cgtggtgtgc aatatctggc tggcggcaga aaagcctgat
      781 aactgccgca ttgttattcg ccctggctgc tacatcactc acgacaccgg gatctacgac
      841 gcggcacaac aacagttgct tgcccgtgat cctgtggcct gcgatctggc aggcgatctc
      901 acatctgcgc tggaactggt cgccatggtg caatcggtgc cggaagctga tcgtgcggtg
      961 gtaaattttg gtaagcgtga ttgcgccttc gacgccggac tgccgcaacc agtcgcccac
     1021 taccgcaacg gcaaatcact ggcatttgat ccacaggcga ttcgcagtac aggtattatg
     1081 gatcagcact gcatgttgca actgtcagct aatagcgacg tgcaagtggg ggatattctg
     1141 gtgtttggca catcgcatcc gtgcctgacc ttcgacaaat ggaaaacgtt gttattgact
     1201 gatgacgact acaacgtact ggcagaatta gacactctct tctaagaacg ccccttcggt
     1261 taattatcga aggggcattt ttattgtgat ctcaatcaca tatccacccg acgaataatt
     1321 cttgcccaac cgctcaccgc cattcctgac cgcttacatc cctaaaataa ccactcagtt
     1381 atttacctta ctttacgcgc gcgtaactct ggcaacatca ctgcaggata gcggtcaatt
     1441 tacctcctca aacgcaacgc aaacctagaa cggcttcggc caactattaa tcaatacatg
     1501 ccaggtttta ctatggatac taaaaagata ttcaagcaca taccctgggt gattctcgga
     1561 atcatcggtg cattctgcct cgcggtagtt gcattacgtc ggggggagca cgtcagtgcc
     1621 ctgtggatcg tggtcgcctc tgtgtcggtg tatctggtgg cttatcgcta ctacagtctg
     1681 tacatcgccc agaaggtgat gaaactcgac cccacgcgcg cgacgcctgc ggttattaac
     1741 aacgacggtc tgaactatgt tccgaccaac cgttacgtgt tgtttggtca ccacttcgcc
     1801 gctatcgccg gtgctggtcc gctggttggt ccggttctcg ccgcgcagat gggctacctg
     1861 cctggcacgc tgtggctgct ggcgggggta gtgctggccg gtgcggtaca ggactttatg
     1921 gtgctgttta tctcctctcg ccgtaacggc gcatctctcg gtgagatgat caaagaagag
     1981 atgggaccag taccggggac tatcgcgctg tttggctgtt tcttaatcat gatcatcatc
     2041 cttgccgtcc tggcgctgat tgtggttaaa gccctggccg aaagtccgtg gggtgtcttc
     2101 accgtttgct caaccgtacc aattgcgctg tttatgggta tctacatgcg ctttatccgt
     2161 ccggggcgtg tgggtgaagt ctctgtcatt ggtatcgtgc tgctggttgc ctctatctac
     2221 ttcggtggcg tgattgctca cgatccgtac tggggtccgg cactgacctt taaagacacc
     2281 accattacct tcgcgctgat tggctatgcg tttgtttccg cgctgctgcc ggtgtggctg
     2341 atcctcgcac cacgcgacta tctggcaacc ttcctgaaaa tcggcgttat cgtcggcctg
     2401 gcgctgggta tcgtggtgct gaacccggaa ctgaaaatgc ctgcgatgac ccagtacatt
     2461 gacggtactg gcccgctgtg gaaaggcgct ctgttcccgt tcctgttcat caccatcgcc
     2521 tgtggtgcgg tatctggctt ccacgcgctg atctcttccg gtacgacgcc gaaactgctg
     2581 gctaacgaaa ccgacgcgcg tttcattggc tacggcgcaa tgctgatgga gtccttcgtg
     2641 gcgattatgg cgctggttgc tgcgtccatc atcgaaccgg gtctttactt cgcgatgaac
     2701 accccgcctg ctggccttgg catcaccatg cctaacctgc atgaaatggg tggcgagaac
     2761 gcgccgatca tcatggcgca gttgaaagac gttaccgcac acgcggcggc gaccgtcagc
     2821 tcctggggct tcgtgatttc gccagagcag atcctgcaaa ccgcgaaaga cattggtgaa
     2881 ccttctgtcc tgaaccgtgc aggtggtgcg ccgacgctgg cggtaggtat cgcgcacgtg
     2941 ttccacaaag tgctgccgat ggctgacatg ggcttctggt atcacttcgg tattctgttc
     3001 gaagccctgt tcatcctgac cgcgctggat gcgggtaccc gttctggccg ctttatgctg
     3061 caagacctgc tgggtaactt catcccgttc ctgaaaaaaa ccgactctct ggttgctggt
     3121 atcatcggta ctgcgggctg tgtgggtctg tggggctacc tgctgtatca gggcgtggtc
     3181 gatccgctgg gcggcgttaa gagcctgtgg ccgctgttcg gtatttccaa ccagatgctg
     3241 gcagccgtag cactggtact gggcaccgtt gtgctgatta agatgaagcg cacccaatac
     3301 atctgggtaa ctgttgttcc ggctgtatgg ctgcttatct gcaccacctg ggcgctgggc
     3361 ctgaaactgt tcagcaccaa cccgcagatg gaaggcttct tctacatggc aagccagtac
     3421 aaagagaaga ttgctaacgg tactgacctg acggcacagc agattgccaa catgaaccac
     3481 atcgttgtga acaactacac taacgcgggt ctgagtattc tgttcctgat tgtggtgtac
     3541 agcatcatct tctacggttt caaaacctgg cttgcggtgc gtaacagcga caaacgtact
     3601 gacaaagaaa ctccgtacgt tccaatcccg gaaggcggcg tgaagatctc ttcgcaccac
     3661 taaccgtgtt tagccccgct tcggcggggc tttgttctta tcagagtgaa ctatgtttgg
     3721 taacttagga caggcaaaaa aatatctcgg ccaggcggcg aagatgttga ttggcattcc
     3781 agactacgac aactacgtcg agcatatgaa gaccaaccat cccgacaagc cgtacatgag
     3841 ctatgaagaa ttcttccgcg aacgccagaa cgcgcgctac ggcggcgatg gtaaaggcgg
     3901 tatgcgctgt tgttaatgga gagagcatga acccgattgc agttacccta ctcaccggtt
     3961 ttttaggcgc gggcaaaacc accctgctgc gccatattct taatgaacag cacggctaca
     4021 aaattgccgt gattgaaaac gaattcggcg aagtctccgt tgatgatcaa ctgattggcg
     4081 accgcgccac gcagatcaaa acgctgacca acggctgcat ctgctgttcg cgctccaacg
     4141 agctggagga cgcgctactc gacctgctgg acaatctcga caagggcaat attcagttcg
     4201 accgtctggt cattgaatgc accggcatgg ccgatcccgg cccgattatt cagacctttt
     4261 tctcccatga gattttatgc cagcgttacc tgctggacgg cgtgattgcg ctggtggatg
     4321 cggtacatgc cgatgagcag atgaaccagt tcaccatcgc ccagtcccag gtgggctacg
     4381 ctgaccgtat tctgctgact aaaaccgacg tcgcaggcga agtagagaaa ctgcgtgaac
     4441 gcctggcgcg catcaacgcc cgcgcaccgg tctacaccgt cacccacggt gacatcgacc
     4501 tgggcctgtt gttcaacacc aacggtttta tgctggaaga aaacgtcgtc agcaccaaac
     4561 cgcgtttcca ctttatcgcg gataaacaaa acgatatttc gtcgattgtg gtggaactgg
     4621 attacccggt agatatcagc gaagtttccc gcgtgatgga aaacctgctg ctggaatcgg
     4681 cggataaact actgcgttac aaagggatgc tgtggattga cggcgaacct agccgcctgc
     4741 tgttccaggg cgtccaacgc ctctacagcg ccgactggga ccgcccgtgg ggcgatgaaa
     4801 caccgcatag tacaatggtg tttatcggga ttcagctgcc ggaggataag attcgggcgg
     4861 cgtttgcggg gttgaggaag taattttaga tgatggccgc catgtggcgg cctgaattat
     4921 tttttattct gatcgcgcag catcgcttca cgtaaaattg cgttcaggcg agtttgatag
     4981 cctttacctg gtcgcttcaa ccattccatc acatcagcat caatacgcac cgaagcctga
     5041 gttttcaatg gccgataaaa cttaccgcgt tctgcgttag accattgctc atcgctggaa
     5101 ggtgggatat cactgtaatc aatatcttcg tcggatttat tcgccagcgc cttcagttcg
     5161 gcctcatgct gcacatcgag tgtagaggag gtccctcgtt tatgtttaac catgctcata
     5221 gcgattccgt tcgttgtgga tcatcaaaca ctaacaccgc atcttcaaag cggatgccat
     5281 gtttcacccg attacctttg gccttatttg catcccattc aaactccatt ggcatagttg
     5341 ccatccctaa ttgtatatac aaaaatgtat gtacaattat tctgcaatta tagcgttgat
     5401 gttggaaata gcatcacgtt tcgaataatc aaccatgaaa ttgacaatgt gtgagttgga
     5461 cttgattcat ccaatagaat ttctcgcctg acatggcaca ctaaacacct cgctgtcgtt
     5521 aagggattac cgtgaaaaac tacaaagtca ttaccccgct ttttcctacc tatgcgcaag
     5581 ttaaggctat gatgaaagcc gtctcaggct attccctgaa agcggtgcgc aatatgatta
     5641 ctgccattca cgagcaaacc ggaacgccgc aaaaacccgt tgactggtcg gaacctgacc
     5701 tgtggatcag cgaacgctta acgggtgaag atgccgacat cgcccgtcgt atttgggata
     5761 ccgataatca catattgaac ccgcgccaca gctacggctg ctacttattc ctcaactatc
     5821 cccagtttga gctgatggaa agcacgccag acgatacctg gcagcccacc tcgcgcgggc
     5881 aaaaattcct gcaagatgac gaaaaaacgc tgcgttcact ggacgatcag gaaggcattc
     5941 tgcaacttct tgaactatta gctggccgcg aaatgtcgcg ccgcgccgat ttattgccgg
     6001 aatggcaggc ctttttgcat cagcattcga aattcgcgtc tgcaagttca gtgaaaagta
     6061 cgctctactc ccgtctctat aaccttatcg accgtgacat ggttgaccgt gaaggcatga
     6121 gctatcgcat caccgatacc gggcgcgcct ggcttggaaa agcgttgccg gcacgaaaaa
     6181 gcgatccacg taaagagctg ctggaagcgg tgaaacgcta caacgagcag caaaaagagc
     6241 tgctgcgcga acaaatcagc acgatgaacc cgtacaaatt tgaacaactg gttggtcaat
     6301 tactggaagc aatgggctac gagcaggtcg aagtgaccaa agcctctggc gacaaaggtg
     6361 tggacgtgat tggccaggtg caggtcggca tcaccactat taccgaagtg gtgcaggtca
     6421 aaagaatgca gaacactatc actcgcccat atatcgacca gttgcgcggg gcgttgcctt
     6481 accacaaagc cattcgcggt acactcatca ccaccgggaa atttgcggcg aaatgtgcag
     6541 aagcggcgct ctttccgggt gccgcgccca ttacgcttat cgacggcgat cgtttactgg
     6601 aactgttaat cgaaaataac gtcggcattc gccgcagcaa tgcagtggaa ttgctggatg
     6661 tggatttgca gttgtttgat gagctggaga tggattaaag ggcgaaatcc gccgcctcta
     6721 ccattacaat agcgacccaa tttatctgaa cagagttcat catggccaaa gcaccaacta
     6781 aaaaagccaa agcaaagaaa ggctttgaag aaacattatg ggatacagca aaccagcttc
     6841 gcggtagcgt tgagtcctcc gaatacaagc acgtggtgtt gagcctcgtg ttcctgaaat
     6901 tcatcagcga taagtttgaa gcacgccgca aaaaaatgat tgccgatggg caagcagact
     6961 tccttgagat ggaagtgttc taccagcagg acaacatttt ctacctgccg gaagaagcgc
     7021 gctggtcttt tatcaaacaa aatgccaaac aggacgatat tgcggtccgt attgacaccg
     7081 ccctctcgac catagagaaa cgtaacccaa cgctgaaagg cgcgctgcca gacaactact
     7141 tcagccgtca gaatctggaa accaaaaaac tggcgtcact gattgatacc atcgacaaca
     7201 tcgaaacgct ggcacacgaa actgacgttg aaacgttatc gaaagaagac ctggtcggac
     7261 gcgtttacga atacttcctc ggtaagtttg ccgcaactga aggcaaaggc ggtggtgagt
     7321 tctacacgcc caaatgtgtg gttacgctgt taactgaaat gctcgaaccc ttccagggca
     7381 aaatttatga cccgtgctgc ggctcggcag ggatgttcgt gcagtcggtg aagtttgtcg
     7441 agagccatca gggtaaaagt cgtgatatcg cgctgtatgg tcaggaactg acagccacga
     7501 cgtataaact ggcaaaaatg aacctcgcca ttcgcggtct ttcagctaac ctcggcgaac
     7561 gaccggcaga caccttcttt agcgaccagc acccggacct gaaagccgac tacattctgg
     7621 cgaacccgcc cttcaacctg aaagactggc gtaatgaagc cgaattaacc aaagacccac
     7681 gttttgctgg gtatcgtatg ccgccaaccg gtaacgccaa ctacggctgg attttgcata
     7741 tgctctccaa gctgtcggct aacggcacag caggttttgt gctggcaaac ggttcgatga
     7801 gttctaacac cagcggtgaa ggcgagatcc gtgcacagat gatcgaaaat gatctgattg
     7861 actgtatgat cgccctacct ggacagttgt tctacaccac gcagatcccg gtgtgtttat
     7921 ggtttatgac caaatcgaag gctgccgatc cggccaaagg ttatcgtgat cgtcagggcg
     7981 agacgctgtt tattgatgcg cgtaacctcg gcaccatgat tagccgcaca actaaagagt
     8041 taacggcgga agatattgcc accatcgccg atacctacca tgcatggcgc agtacgccgg
     8101 aagaactggc tgcacggatt gcgcgtggtg acagcaagct ggaaaaatat gaagaccagg
     8161 ctggcttctg caaagttgcg accctgcaag acattaaaga taacgactac gttctgacac
     8221 cgggccgcta tgtaggtgca gccgagcagg aagaggacgg cgtggcattt gagacgaaaa
     8281 tgcgcgaatt gtcgaagacg ttgtttgagc agatgaagca ggcggaagaa ctggatcgtg
     8341 cgattcgcca gaatctggag gtgctgggtt atggggagta agtggataga agtatcactg
     8401 ggcgagattt ctgaaaaaat tggagatgga atccacggaa cgccaactta taacaattca
     8461 ggtaactatt actttattaa tggaagtaat ttaattgata attcaataa
//
LOCUS       DAZFLD010000193         8414 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.194, whole genome shotgun sequence.
ACCESSION   DAZFLD010000193 DAZFLD010000000
VERSION     DAZFLD010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8414
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.194"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(41..466)
                     /locus_tag="ACHKBL_003950"
     CDS             complement(41..466)
                     /locus_tag="ACHKBL_003950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5820750.1"
                     /translation="MIRIIVFLLFFLSGCTSQKEYSNIVRIENRCSSDIDVFIQGYVS
                     GSTLIYQHNIHLPVGDSDVLVYFSTADIDDGFKYEGAFMIYKDSRGELDYPFTISLSG
                     REKKFGKNDLIRLSRDISTKDDRKYGVIRYIIDSQEICP"
     gene            complement(459..1811)
                     /locus_tag="ACHKBL_003951"
     CDS             complement(459..1811)
                     /locus_tag="ACHKBL_003951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001402492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAAR domain-containing protein"
                     /protein_id="HFO5820751.1"
                     /translation="MGTGYFLVRGDKTTCGGKIIEGADDHTIMGIPQARDMDRVTCGR
                     YPGMFIIVGGVPETDIHGRLMAGTLDSQSSCPCKARFIASMMDDTYETDDGESEAEQH
                     AQSAKKDLTSGSDSSSDDVKLDYRIKLSGNKILTPLNIPDYKEMISGGSTKNTEKIDF
                     TITNKGDEAEALSLEVLDGNEVIYSERQTGKYCDKGEHAWQWDGYSNQGILDTTKIKS
                     KSLLVRLIALCGDMMIKVDRNNKVVDITWRLSVKDSGTKGSNPELSPIPYSDLVSLTK
                     NGVEFYWSRNGSRGGGIGGNIVTAIGVFKVNVKAEINITPSMRTFDLISSLDPDMQSS
                     VSLLGFEKIYHNYGGYYNNSKDEIKALLYSNERYKWDSAHEMGHKVMDKYGSESWTHK
                     GTSTRGQSTIPNTPMPSSGEIDIMKYADSYAPDRYKRLVAADEDVQALIWLSRVSFND
                     "
     gene            complement(1817..3427)
                     /gene="tssA"
                     /locus_tag="ACHKBL_003952"
     CDS             complement(1817..3427)
                     /gene="tssA"
                     /locus_tag="ACHKBL_003952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001299109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssA"
                     /protein_id="HFO5820752.1"
                     /translation="MSELLQKLTRSCFADRDALDSARTQAALWQTWLLPVTADTPVGE
                     DPGYHDDFLRIRDEMNKLSGADTDLICQLAESLLLTQAKDVRIATYYIWARLHRDGER
                     GLAEGLALLAGLVERFGAQLLPSRPASRKMALEWLAGEKMLDSLARYPEVAKEDFANI
                     VAALNQLTVSFTAWPEDQHSPSLMPLINALESRLAQSGRMNAVVPQNSSGVPAPSSPV
                     DAPQVQTITSGRDLLDQAKVLARYLNEQPQGWLSAHRLMKTLRWDTVHELPPDVDGKT
                     RLAPPRTESRNQLKRLYAQQNWTELLEQADLMFSTGVSHFWLDIQWYLHQALTKAGAP
                     WDRWTAVIRQDLALLLERLPGLENLAWNDGTPFADELTRNWIAQQVMMREDGAWLAGK
                     AAVPTDDATNDVLALEPEALEMADSQGVEAALGWIQTRPGITTARQRLLLRLLMARVA
                     EQYGKNEMALLLLEELDIAAQGLTLTQWEPDLLFEVKARQLKLLRLRAHRYADKALLN
                     RKMEILLGTLVTIDPVRAAVLCDTQHKD"
     gene            complement(3420..6746)
                     /locus_tag="ACHKBL_003953"
     CDS             complement(3420..6746)
                     /locus_tag="ACHKBL_003953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001335663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImcF-related family protein"
                     /protein_id="HFO5820753.1"
                     /translation="MTVAVVVALCTFIHRKPALLGVEPGSSQQMYWMAGVIAAGVVFL
                     IIMGVYHLLNRHAAVQSFELHRQLAAGDDYRAVTTKKADSRHPAMVELAAFLRDRYGP
                     FWRRKIRLLLVTGEPEQAEAIAPGLTGQHWLEGDHTVLIYGGRPSTEPDVALLTALKK
                     LRRSRPLDGIIWPLTEEQSRQTAQLDKGWRELINGGKRLGFQAPLYLWQVCDNGDYQT
                     GRPLQSVGCLLSERCTPEQLAAMLEAQTLPLTEQGMSQLLTDNRHDFLLRLAHTLAER
                     GIAHWQSILKPLLAGGAFSSLRLRGLMFSPPLTAVPEAAPHAWLPSPVWAGVTGDNAR
                     GRTVGFPWLRITLMSAVCVLAIWGAGMTTSFFANRALVQETSIQTARALDIRLPLSEQ
                     LVALHTLQGELERLQYRIREGAPWYQRFGLERNQQLLAAAFPGYAQAANRLVRDVAVD
                     HLQQQLNAFVALPPNSPQRTATGEQRYKQLKALLMTSRPEKADAAFFSTTLMADDLRY
                     AGVPEGVRQSVLPSLLTFWMANLPEHPQWKTSPPPELTGAVRKILLRQIGVRNAENTL
                     YQNVLQQVSRNYADMTLADMTGDTLTESLFSTEQTVPGMFTRQAWEGQVREAIEQVVT
                     ARREEIDWVLSDRQQDTSADISPDMLRNRLTSRYFTDFAGSWLAFLNSIRWKKEDSLS
                     GILDQLTLMADARQSPLIALTDTLAWQAAAGRENRGLSDSLAKSAQELFNGKEKTPQQ
                     SREGDVPVGPLDKTFAPLLRLLGDKAGGGDSQLSLQTYLTRVTRVRLKLQQVTNAPDP
                     QEMTQQLAQTVLQGKTVDLTDTRDYGSLIAASLGEEWSGFGQALFVRPVEQSWRQVLT
                     PAADSLNRQWQRAIVSHWDQDFAGRYPFKASQNDASLPLLAQYLRDDGRINQFIAANL
                     SGVLKREGRYWVADAMNTQGLTVNPDFIRALNRLRDVADTAFASGDAGIHFELRAKPA
                     RDVMKTHLVIDGQELEYFNQKERWQRFNWPDEQWQPGASLSWTSTQAMERILADYRGS
                     WSLIRLLEQAQVTPVDSSTFKVVWKAQDGLPLNYLLRVEQGKGPLALLELKNFRLPGQ
                     VFLTGRSMKDAEEYGEDADE"
     gene            complement(6803..8017)
                     /locus_tag="ACHKBL_003954"
     CDS             complement(6803..8017)
                     /locus_tag="ACHKBL_003954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001145249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5820754.1"
                     /translation="MPIRLNTIPDAEPYPAPPARIKWLSALLLMLSVGVAITSLFASD
                     ELAKNGPYFWGLACGVPAFIWSLVGSVRWLVFVTQHIRADAWNQRREEVILQETRRGR
                     RALQILSFSVQTALNGDSVAETTTAFLARHQVLNTYADLRGEDTVRRSVIPVLTNKPV
                     TGRLSEIISRLFIDIRPQLFLLPPDFHINVLLEIDAPLSGASVRTIWQDEWQKAGLPE
                     ARLFSAPEPGLAAVDDWLDNFIQEKAVLLVISVRLEPKNPERTAESATALLLANRLTQ
                     TALTPLALLHRPERITDTEMMASGIAQALDWMPVQPDAISGMWTVELNREQRASLLSS
                     NQPFTQEALMYELDAFLGCSGPAAPWLSVAAATLAAIQSQHPQLTLSGVQNGHYSWAT
                     MVSPFVSPQEAS"
     gene            complement(8123..>8414)
                     /locus_tag="ACHKBL_003955"
     CDS             complement(8123..>8414)
                     /locus_tag="ACHKBL_003955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5820755.1"
                     /translation="DKVLITACNHWVLNSRDIIDIFNLSIKHDDYGEINRMYYWLPCE
                     IRGRLMYDGIEWSFVINAAATAMWMSNDKEIYWGCNKKACDNFFLLPYDGMR"
BASE COUNT         1821 a         2439 c         2132 g         2022 t
ORIGIN      
        1 taggcaataa ttaataattg tgaacgaata acttaaaaca tcacgggcaa atttcctgac
       61 tgtcgattat atatcttata actccgtatt ttctgtcgtc ttttgttgaa atatctcgtg
      121 ataatctaat taagtcattc ttcccaaatt ttttttcgcg ccctgacaat gaaatggtga
      181 atggataatc caattcaccg cggctatctt tatatatcat gaatgccccc tcatatttaa
      241 aaccatcatc aatatctgcc gtggaaaaat atactaatac atcactatca ccaactggca
      301 agtgtatatt atgctgataa atcagcgtag aaccggaaac atatccctga ataaagacat
      361 cgatatctga ggagcatcta ttctcaatcc tgactatatt ggaatactct ttttgtgaag
      421 tacacccact caaaaaaaac aataaaaaaa caattattct aatcattaaa actcaccctg
      481 ctcaaccaga tcaatgcctg aacatcttca tctgccgcga ccaatctctt atatctatca
      541 ggtgcatagc tatctgcata tttcattata tcaatctctc ctgaagatgg cattggagta
      601 ttagggatgg tactttgacc ccgcgtggat gtccccttat gtgtccatga ttcagaacca
      661 tatttatcca taactttatg tcccatctca tgagcactat cccatttata tctttcatta
      721 ctatatagaa gagcctttat ttcatccttg ctgttattat aatagccacc atagttatgg
      781 tatatttttt caaaccccaa aagacttacc gacgactgca tatctggatc taacgatgat
      841 atcaaatcaa aagttcgcat agatggagta atatttatct cagcctttac atttacttta
      901 aaaaccccaa tcgctgtaac gatattacca ccaattcccc cgccgcgact tccatttcgt
      961 gaccagtaaa actccacgcc atttttagta agactaacta aatcgctata aggtattggc
     1021 gataactcag ggttactgcc ttttgtaccg ctatccttaa cagataatct ccatgttata
     1081 tcgaccactt tattatttct gtcaactttt atcatcatat caccacataa agcaatcagt
     1141 cttacgagta gtgatttgct ttttatctta gttgtatcca gtatgccttg attactgtag
     1201 ccatcccatt gccaggcatg ctctccctta tcacaatatt taccggtctg ccgttcacta
     1261 taaatcactt cattaccatc aagcacttcc agagaaagcg cttcagcctc atcgccttta
     1321 tttgtgatgg taaaatctat tttttctgta ttcttcgtac taccaccgct aatcatttct
     1381 ttataatcag gtatattcag tggcgtcagt atcttattac ctgataattt aattctgtaa
     1441 tccagtttta catcatcaga ggatgaatca gaaccagaag tcagatcctt tttcgccgac
     1501 tgcgcatgtt gttccgcttc actttccccg tcatccgtct catacgtatc atccatcatt
     1561 gacgcaataa accgtgcctt acacgggcaa ctgctctgac tgtccagcgt ccccgccatc
     1621 agcctgccgt gaatatctgt ttcaggaacg ccgcccacaa taataaacat tcccggatat
     1681 ctgccgcagg tgacccggtc catatcccgc gcctgcggga tacccatgat cgtatggtcg
     1741 tccgcgcctt caataatttt gccgccgcag gtggttttgt cccccctgac cagaaagtag
     1801 cctgttccca ttgccatcag tccttatgtt gcgtatcaca caataccgct gcccgtaccg
     1861 gatctattgt caccagcgtt cccagcagga tttccatttt tcggttcagc agggctttat
     1921 ccgcatagcg gtgcgcccgc aggcgcagca gtttcagctg tcgggctttt acctcaaaca
     1981 gcaggtccgg ctcccactgc gtcagcgtca gcccctgtgc ggcgatatcc agttcttcca
     2041 gcagcaggag cgccatctcg tttttaccgt actgttccgc cacccgcgcc atcagcagtc
     2101 gcaataacag gcgctggcgt gccgtggtga tgcccggacg ggtctgtatc cagcccagcg
     2161 ccgcttccac gccctgactg tctgccatct ccagcgcttc cggctccagc gccagtacgt
     2221 cgtttgtggc gtcgtctgtc ggtacagcgg ctttccccgc aagccatgcg ccgtcttccc
     2281 gcatcatgac ctgctgcgct atccagttgc gcgtcaactc atcggcgaac ggcgtgccgt
     2341 cgttccatgc cagattttcc agccccggca ggcgttccag tagtaacgcc aggtcctgac
     2401 ggataaccgc tgtccacctg tcccacggtg cgcccgcctt agtcagcgcc tgatgcagat
     2461 accactgaat gtccagccag aaatggctga cgccggtgct gaacatcagg tcggcctgct
     2521 ccagcagttc cgtccagttc tgctgagcat acagccgctt aagctggttg cgtgattctg
     2581 tgcgcggcgg ggccagccgg gtttttccgt ccacatccgg cggcagttca tgcaccgtgt
     2641 cccagcgcag ggttttcatc agccggtgtg ctgaaagcca gccctgcggc tgttcgttca
     2701 gataacgggc cagcactttc gcctggtcga gcaggtcgcg cccggaagtg atggtctgta
     2761 cctgtggcgc atccaccggg gaagacggtg ccggaacgcc gctgctgttc tgcggcacca
     2821 ccgcgttcat cctacccgac tgcgccagac gtgattccag cgcgttaatc agcggcatca
     2881 gggaggggga atgctggtct tcgggccatg cagtgaagga aaccgtcagt tgattaagag
     2941 cggcaacaat gttcgcaaaa tcctcttttg ccacttccgg gtagcgggca aggctgtcga
     3001 gcatcttttc ccctgccagc cactccagcg ccatttttcg gcttgccggg cgcgagggca
     3061 gcagttgcgc accgaagcgt tccaccagtc cggctaacag cgccagcccc tcagccagtc
     3121 cgcgttcgcc atcgcggtgc agtcgcgccc agatatagta agtggcgatg cgcacgtctt
     3181 ttgcctgcgt cagcagcagg gattcggcaa gctggcagat aaggtccgta tccgcgccgg
     3241 aaagtttgtt catctcgtcg cggatacgca ggaaatcgtc gtggtagccg gggtcttccc
     3301 cgaccggcgt atctgccgtc accggcagca gccatgtctg ccacagcgcc gcctgtgttc
     3361 gcgccgagtc cagtgcgtcc cggtcggcaa aacagctacg ggtcagcttt tgcaacagtt
     3421 cactcatcgg cgtcttcccc gtattcttcc gcatccttca tactcctgcc ggtcaggaac
     3481 acctgtcccg gcaggcggaa gtttttcagt tccagcagcg ccagcggccc tttaccctgt
     3541 tcaacccgca gcaggtaatt cagcggcagg ccgtcctgcg ctttccacac cactttaaag
     3601 gtactgctgt ccaccggtgt cacctgcgcc tgttccagca ggcgaataag gctccagctt
     3661 ccccgataat ccgccagtat gcgttccatc gcctgcgtac tggtccagct cagcgatgcg
     3721 ccgggttgcc actgttcatc cggccagtta aaacgctgcc agcgttcctt ctggttgaaa
     3781 tattccagct cctgcccgtc aatcaccaga tgcgtcttca tcacgtcgcg cgccggtttt
     3841 gcccgcagtt caaaatgtat ccctgcatcg ccgctggcaa aggcggtatc ggccacatcg
     3901 cgcaggcggt tcagggcgcg aataaagtcc ggattgactg tcagcccctg cgtgttcatg
     3961 gcgtcagcca cccagtagcg gccttcacgt ttcagtacgc cggaaagatt ggcggcgata
     4021 aactggttga tgcgcccgtc atcgcgcagg tactgcgcca gcagggggag ggaggcatcg
     4081 ttttgtgagg ctttgaacgg gtagcgacca gcgaagtcct gatcccagtg gctgacaatc
     4141 gcccgctgcc actggcggtt caggctgtct gccgcaggcg tcagcacctg ccgccatgac
     4201 tgctccaccg ggcggacgaa cagcgcctga ccgaagccac tccactcttc acccagactg
     4261 gcggctatca ggctgccata gtcgcgggtg tcggtaaggt caacggtttt gccttgtaag
     4321 accgtctgcg ccagttgctg ggtcatctcc tgcgggtcag gggcgttggt cacctgttgc
     4381 agtttgaggc gcacgcgggt gactcgggtg aggtaggtct gcaggctcag ctggctgtcg
     4441 ccgcctcctg ccttatcgcc aagcaaccgc agcagcggcg cgaaggtttt atccagcggc
     4501 ccgacaggca cgtcgccttc acgggattgc tgcggcgttt tctccttgcc gttaaacagt
     4561 tcctgtgccg atttcgccag cgagtctgac aggccacggt tttccctgcc agccgccgcc
     4621 tgccacgcga gggtgtctgt cagcgcaatc agcggtgact ggcgggcatc ggccatcagc
     4681 gtcaactggt cgagaatgcc ggagagcgag tcttcctttt tccagcgaat gctgttgaga
     4741 aacgccagcc agcttccggc aaagtcggta aagtagcgtg aagtgagacg gttacgcagc
     4801 atatccggcg agatatccgc agaggtatcc tgctggcggt cgctgagtac ccagtcgatt
     4861 tcctcgcgcc gcgccgtcac cacctgctcg atggcttccc tgacctgtcc ttcccacgcc
     4921 tgacgggtaa acatccccgg caccgtctgc tccgtactga aaagagattc ggtgagcgta
     4981 tccccggtca tgtccgccag cgtcatatcg gcgtagttgc gggacacctg ttgcagtacg
     5041 ttctggtaga gagtgttttc ggcattgcgc acaccaatct ggcgcagcag gattttgcgc
     5101 actgcgccgg tcagttccgg tggcggcgaa gttttccact gtgggtgttc cggcagattc
     5161 gccatccaga aggtcagcag tgacggcaac acgctctgac gcacaccttc cggtacacct
     5221 gcatagcgca ggtcatctgc catcagcgtg gtactgaaaa aggcagcgtc ggccttttcc
     5281 gggcgggaag tcatcagcaa tgccttaagc tgcttatagc gctgttcgcc ggtggcggta
     5341 cgctgcggac tattgggcgg cagcgcgaca aaggcattca gttgctgttg cagatggtca
     5401 acggccacgt cgcgcaccag ccggtttgcc gcctgcgcat agccgggaaa ggcggcggcg
     5461 agcagttgct ggttacgttc aaggccaaaa cgctgatacc acggcgcacc ctcccggata
     5521 cggtactgca ggcgctccag ttcaccctgc agggtatgca gcgccaccag ttgttcagac
     5581 agcggcagac ggatatcgag tgcacgcgcc gtctggatgc tggtttcctg aacaagtgcg
     5641 cggttggcga agaacgaggt cgtcattccg gccccccata tcgccagtac gcagacagca
     5701 gacatcagcg tgatacgcag ccacggaaag cccaccgtgc gaccccgcgc gttatcgccg
     5761 gtcacgcccg cccagaccgg tgacggcagc cacgcatgag gtgcggcttc cggtacggcg
     5821 gtaagcggcg ggctgaacat caggccacgc aggcgcaggg aggaaaatgc gccgcctgcc
     5881 agcagcggtt tcaggatact ttgccagtga gcgatacccc gctctgcaag ggtatgggcc
     5941 agacgcagca ggaaatcatg gcggttatcg gtaagcagct gcgacatgcc ctgttccgtc
     6001 agcggcaggg tttgtgcttc cagcattgca gccagttgtt ccggggtaca gcgttccgac
     6061 agcaggcagc cgacgctttg cagggggcgt ccggtctgat aatcaccgtt gtcacagacc
     6121 tgccacaaat agagtggagc ctgaaaaccg agtcgcttac cgccgtttat cagttcgcgc
     6181 cagcctttgt cgagttgcgc cgtctggcgg ctctgttctt ctgtcagcgg ccagatgatg
     6241 ccgtccagcg gacggctgcg gcgcagtttt tttaaggcgg tcagtagcgc gacatcaggc
     6301 tctgtagatg gcctgccgcc atatatcagc accgtgtggt cgccctccag ccagtgttgc
     6361 ccggtcagcc ccggcgcgat ggcctctgcc tgttcaggct cgccggtcac cagcagcagg
     6421 cggattttac gccgccagaa tgggccgtag cggtcgcgca ggaaggcggc gagttccacc
     6481 attgccggat gacggctgtc ggctttcttt gtcgtcaccg cccgatagtc atctccggcg
     6541 gcaagctgtc tgtggagttc aaagctctgt acggcagcgt ggcggttcag cagatgataa
     6601 acccccatga taatcagaaa gaccacacct gcggcaatca cgccagccat ccagtacatt
     6661 tgctgtgaac tacccggttc aacgcccagc aatgccggtt tacggtgaat aaacgtgcaa
     6721 agcgcaacca caacggccac ggtcatgacg atggcaggcc ctgaaaccga acggggccgg
     6781 agacggttta atcccggaac cactatgatg cctcctgagg ggaaacgaat ggcgacacca
     6841 tggtcgccca tgaataatgc ccgttctgaa cgccgctgag cgtcagttgg gggtgttgtg
     6901 attgtattgc cgccagcgtt gccgcagcga cggacagcca cggcgctgcc ggtccggagc
     6961 accccagaaa ggcatccagc tcgtacatca gggcttcctg agtaaacggc tggttagatg
     7021 acagcagcga ggcgcgctgt tccctgttca gttcgactgt ccacatgccg gatatggcat
     7081 ctggctgcac cggcatccag tccagcgcct gtgcaatgcc tgacgccatc atctccgtat
     7141 ctgtgatacg ttcaggacga tgcagcaggg ccagcggcgt cagcgcggtt tgcgtcagtc
     7201 ggtttgccag caacagcgcg gtggcggatt ctgccgtccg ttccgggttc ttcggttcca
     7261 gacgcacgga aatcaccagc agtacggcct tttcctgaat aaagttgtcc agccagtcgt
     7321 ccacggcggc cagccccggc tccggggcag aaaacagacg ggcttcgggg agtccggctt
     7381 tttgccattc gtcctgccag atagttctca cggaggcccc tgaaagcggc gcatcaattt
     7441 ccagtagtac gttgatatga aaatcagggg gcagcagaaa caattgcggt ctgatatcaa
     7501 taaaaaggcg ggaaataatt tcggataaac gcccggtaac cggtttattg gttaatacgg
     7561 gaatgacact gcgacgcacg gtatcttctc cgcgcaaatc agcgtaagta ttcaacacct
     7621 gatgacgcgc cagaaatgct gttgtagttt ctgcgacgga atcaccattc agcgcggttt
     7681 gtacggaaaa actgagtatc tgcaacgccc gtcgtccccg ccgtgtttcc tgcaaaatga
     7741 cttcttcgcg acgttgattc caggcatcgg cacgaatatg ttgagtgaca aataccagcc
     7801 agcgcaccga accgaccagt gaccagataa aagcaggcac gccgcaggcc agcccccaga
     7861 aatatgggcc gttttttgcc agttcatcgc tggcgaataa tgaggttatt gccacaccaa
     7921 cagacagcat cagtaataag gctgacagcc atttaatgcg ggctggcggt gcgggatagg
     7981 gttctgcgtc gggaatggta ttaagtcgga tgggcattgt aataatccgt tattctgaaa
     8041 tatgaataca atgaatctca aggtgcttga actatctgat taattcactt gtccataatg
     8101 tttttgcttt attaactgtg atttacctca tcccatcata tggtaataaa aagaaattat
     8161 cgcatgcttt tttattgcat ccccagtaaa tctctttatc attgctcatc cacattgctg
     8221 ttgcagcagc attgataaca aaactccatt ctattccgtc atacattagt ctccctctaa
     8281 tttcacaagg taaccaataa tacattctgt ttatttcgcc ataatcatca tgctttatac
     8341 ttagattgaa tatgtctata atatctctag aattaagcac ccaatgatta cacgcagtaa
     8401 taagcacctt atct
//
LOCUS       DAZFLD010000194         8398 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.195, whole genome shotgun sequence.
ACCESSION   DAZFLD010000194 DAZFLD010000000
VERSION     DAZFLD010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8398
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.195"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            98..640
                     /gene="ecpR"
                     /locus_tag="ACHKBL_003956"
                     /gene_synonym="matA"
     CDS             98..640
                     /gene="ecpR"
                     /locus_tag="ACHKBL_003956"
                     /gene_synonym="matA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECP biosynthesis operon DNA-binding
                     transcriptional regulator EcpR"
                     /protein_id="HFO5820756.1"
                     /translation="MECQNRSDKYIWSPHDAYFYKGLSELIVDIDRLIYLSLEKIRKD
                     FVFINLNTDSLTEFINRDNEWLSAVKGKQVVLIAARKSEALANYWYYNSNIRGVVYAG
                     LSRDIRKELAYVINGRFLRKDIKKDKITDREMEIIRMTAQGMLPKSIARIENCSVKTV
                     YTHRRNAEAKLYSKLYKLVQ"
     gene            714..1301
                     /gene="ecpA"
                     /locus_tag="ACHKBL_003957"
                     /gene_synonym="matB"
     CDS             714..1301
                     /gene="ecpA"
                     /locus_tag="ACHKBL_003957"
                     /gene_synonym="matB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="common pilus major fimbrillin subunit EcpA"
                     /protein_id="HFO5820757.1"
                     /translation="MKKKVLAIALVTVFTGTGVAQAADVTAQAVATWSATAKKDTTSK
                     LVVTPLGSLAFQYAEGIKGFNSQKGLFDVAIEGDSTATAFKLTSRLITNTLTQLDTSG
                     STLNVGVDYNGAAVEKTGDTVMIDTANGVLGGNLSPLANGYNASNRTTAQDGFTFSII
                     SGTTNGTTAVTDYSTLPEGIWSGDVSVQFDATWTS"
     gene            1358..2026
                     /gene="ecpB"
                     /locus_tag="ACHKBL_003958"
                     /gene_synonym="yagY"
     CDS             1358..2026
                     /gene="ecpB"
                     /locus_tag="ACHKBL_003958"
                     /gene_synonym="yagY"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone EcpB"
                     /protein_id="HFO5820758.1"
                     /translation="MKKHLLPLALLFSGISPAQALDVGDISSFMNSDSSTLSKTIQNS
                     TDSGRLINIRLERLSSPLDDGQVIAMDKPDELLLTPASLLLPAQASEVIRFFYKGPAD
                     EKERYYRIVWFDQALSDAQRDNANRSAVATASARIGTILVVAPRQANYHFQYANGSLT
                     NTGNATLRILAYGPCLKAANGKECKENYYLMPGKSRRFTRVDTADNKGRVALWQGDKF
                     IPVK"
     gene            2052..4577
                     /gene="ecpC"
                     /locus_tag="ACHKBL_003959"
                     /gene_synonym="yagX"
     CDS             2052..4577
                     /gene="ecpC"
                     /locus_tag="ACHKBL_003959"
                     /gene_synonym="yagX"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher EcpC"
                     /protein_id="HFO5820759.1"
                     /translation="MPLRRFSPGLKAQFAFGMVFLFVQPDASAADISAQQIGGVIIPQ
                     AFSQALQDGMSVPLYIHLAGSQGRQDDQRIGSAFIWLDDGQLRIRKIQLEESEDNASV
                     SEQTRQQLMTLANAPFNEALTIPLTDNAQLDLSLRQLLLQLVVKREALGTVLRSRSED
                     IGQSSVNTLSSNLSYNFGVYNNQLRNGGSNTSSYLSLNNVTALREHHVVLDGSLYGIG
                     SGQQDSELYKAMYERDFAGHRFAGGMLDTWNLQSLGPMTAISAGKIYGLSWGNQASST
                     IFDSSQSATPVIAFLPAAGEVHLTRDGRLLSVQNFTMGNHEVDTRGLPYGIYDVEVEV
                     IVNGRVISKRTQRVNKLFSRGRGVGAPLAWQIWGGSFHMDRWSENGKKTRPAKESWLA
                     GASTSGSLSTFSWAATGYGYDNQAVGETRLTLPLGGAINVNLQNMLASDSSWSNIASI
                     SATLPGGFSSLWVNQEKTRIGNQLRRSDADNRAIGGTLNLNSLWSKLGTFSISYNDDR
                     RYNSHYYTADYYQSVYSGTFGSLGLRAGIQRYNNGDSSANTGKYIALDLSLPLGNWFS
                     AGMTHQNGYTMANLSARKQFDEGTIRTVGANLSRAISGDTGDDKTLSGGAYAQFDARY
                     ASGTLNVNSAADGYINTNLTANGSVGWQGKNIAASGRTDGNAGVIFDTGLENDGQISA
                     KINGRIFPLNGKRNYLPLSPYGRYEVELQNSKNSLDSYDIVSGRKSHLTLYPGNVAVI
                     EPEVKQMVTVSGRIRAEDGTLLANARINNHIGRTRTDENGEFVMDVDKKYPTIDFRYS
                     GNKTCEVALELNQARGAVWVGDVVCSGLSSWAAVTQTGEENES"
     gene            4567..6210
                     /gene="ecpD"
                     /locus_tag="ACHKBL_003960"
     CDS             4567..6210
                     /gene="ecpD"
                     /locus_tag="ACHKBL_003960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial adhesin EcpD"
                     /protein_id="HFO5820760.1"
                     /translation="MRVNLLIAMIIFALIWPATALRAAVSKTTWADAPAREFVFVENN
                     SDDNFFVTPGGALDPRLTGANRWTGLKYNGSGTIYQQSLGYIDNGYNTGLYTNWKFDM
                     WLENSPVSSPLTGLRCINWYAGCNMTTSLILPQTTDASGFYGATVTSGGAKWMHGMLS
                     DAFYQYLQQMPVGSSFTMTINACQTSVNYDASSGARCKDQASGNWYVRNVTHTKAANL
                     RLINTHSLAEVFINSDGVPTLGEGNADCRTQTIGSRSGLSCKMVNYTLQTNGLSNTSI
                     HIFPAIANSSLASAVGAYDMQFSLNGSSWKPVSNTAYYYTFNEMKSADSIYVFFSSNF
                     FKQMVNLGISDINTKDLFNFRFQNTTSPESGWYEFSTSNTLIIKPRDFSISIISDEYT
                     QTPSREGYVGSGESALDFGYIVTTSGKTAADEVLIKVTGPAQVIGGRSYCVFSSDDGK
                     AKVPFPATLSFITRNGATKTYDAGCDDSWRDMTDALWLTTPWTDISGEVGQMDKTTVK
                     FSIPMDNAISLRTVDDNGWFGEVSASGEIHVQATWRNIN"
     gene            6179..6889
                     /gene="ecpE"
                     /locus_tag="ACHKBL_003961"
     CDS             6179..6889
                     /gene="ecpE"
                     /locus_tag="ACHKBL_003961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone EcpE"
                     /protein_id="HFO5820761.1"
                     /translation="MFRRRGVTLTKALLTVVCMLAAPLTQAISVGNLTFSLPSETDFV
                     SKRVVNNNKSARIYRIAISAIDSPGSSELRTRPVDGELLFAPRQLALQTGESEYFKFY
                     YHGPRDNRERYYRVSFREVPTRNLTKRSPTGGEVSTEPVVVMDTILVVRPRQVQFKWS
                     FDQVTGTVSNTGNTWFKLLIKPGCDSTEEEGDAWYLRPGDVVHQPELRQPGNHYLVYN
                     DKFIKISDSCPAKPPSAD"
     gene            7202..7531
                     /gene="ykgJ"
                     /locus_tag="ACHKBL_003962"
     CDS             7202..7531
                     /gene="ykgJ"
                     /locus_tag="ACHKBL_003962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HFO5820762.1"
                     /translation="MSNLNPCMTCGACCAFFRVSFYWAEADDAGGTIPARLTEQISPF
                     HRCMSGTNQKNPRCIALAGTPGKNACCTIYENRSSTCREFAMSGENGEVNEACNRARA
                     KYGLTPL"
     gene            complement(7543..7709)
                     /locus_tag="ACHKBL_003963"
                     /pseudo
     CDS             complement(7543..7709)
                     /locus_tag="ACHKBL_003963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000885624.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            complement(7780..8394)
                     /gene="yagU"
                     /locus_tag="ACHKBL_003964"
     CDS             complement(7780..8394)
                     /gene="yagU"
                     /locus_tag="ACHKBL_003964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YagU family protein"
                     /protein_id="HFO5820763.1"
                     /translation="MNIFEQTPPNRRRYGLAAFIGLIAGVVSAFVKWGAEVPLPPRSP
                     VDMFNAACGPESLIRAAGQIDCSRNFLNPPYIFLRDWLGLTDPNSAVYTFAGHVFNWV
                     GVTHIIFSIVFAVGYCVVAEVFPKIKLWQGLLAGALAQLFVHMISFPLMGLTPPLFDL
                     PWYENVSEIFGHLVWFWSIEIIRRDLRNRITHEPDPEIPLGSNR"
BASE COUNT         2234 a         2144 c         2130 g         1890 t
ORIGIN      
        1 ttactaaaga aactgaatgt acctgtaaaa attacaggtt tggaaagtag tgacatggca
       61 aaatgattac agcagggact atgaggttaa aaaccatatg gaatgtcaaa accgttctga
      121 taaatacatc tggtctcccc atgacgccta cttctataaa ggactatctg aactgattgt
      181 ggatatcgac agattaattt atctatcgct ggagaaaatc agaaaagatt tcgtgtttat
      241 caatctcaat acggattctt taactgagtt tataaaccgt gataatgagt ggttatccgc
      301 ggtaaagggg aaacaggtcg tattgattgc ggccagaaag tcagaagcct tagcaaatta
      361 ttggtattac aacagcaata ttaggggcgt ggtatacgct ggactgagtc gtgatattag
      421 aaaagaactg gcctatgtga ttaatggcag gttcctgaga aaagatatta agaaagataa
      481 aatcactgac cgggaaatgg aaattatccg catgacggct cagggaatgc tgcctaaatc
      541 gattgccaga attgaaaatt gtagtgtgaa gacagtgtat acccatcggc ggaatgcaga
      601 ggccaagctg tactcaaaat tatataagtt ggttcagtaa actccaagca agttagtttt
      661 aaaaatgact caccgggact tcatgtcctc aattcaactc gggaagaaaa gcaatgaaaa
      721 aaaaggttct ggcaatagct ctggtaacgg tgtttaccgg tacaggtgta gcgcaggctg
      781 ctgacgtaac agctcaggct gtagcgacct ggtcagcaac agccaaaaaa gacaccacca
      841 gtaagctggt tgtgacgcca ctcggtagcc tggcgttcca gtatgccgaa ggcattaaag
      901 gttttaactc acaaaaaggt ctatttgacg tggctatcga gggtgactca acggctaccg
      961 cctttaaact gacctcacgc cttatcacca acaccttaac ccagttggat acctcaggtt
     1021 ccacactgaa tgtgggcgtg gattataacg gcgcggcagt cgaaaaaact ggcgataccg
     1081 tgatgatcga taccgccaac ggcgtactgg gcggcaacct tagcccactg gctaacggtt
     1141 acaatgccag caatcgtacc accgcacagg atggtttcac tttctccatc atcagcggca
     1201 ccaccaatgg taccaccgca gtaaccgatt acagcactct accggaaggc atctggagcg
     1261 gcgacgttag cgtacagttc gacgctacct ggaccagtta atctctctga tgtaccagca
     1321 ggggtacccc cctgctttat tcctggatgg aattgttatg aaaaagcacc ttctgcctct
     1381 cgctctgctg ttttccggaa tatcaccggc ccaggcgctg gatgtcggcg atatatcatc
     1441 gtttatgaac agtgacagca gcacgctaag caaaacgatc caaaacagta ccgacagtgg
     1501 ccgcctcatc aatatccgtc tcgaacggct ctcttcaccg cttgacgacg ggcaggttat
     1561 cgcaatggac aagccggatg agttgctact cactcccgcc agcttgctgc tacccgccca
     1621 agccagcgaa gtgatccgct tcttctataa gggaccagca gatgaaaaag agcgctacta
     1681 ccgcattgtc tggtttgatc aggccctcag tgatgcgcag cgcgataatg ccaaccgcag
     1741 cgctgtggcc actgcttccg cccgcatcgg caccattctg gtcgtcgcac cccgccaggc
     1801 aaactaccac tttcagtacg ccaacggctc cctgacaaat acaggaaatg cgacgctgcg
     1861 gatcctcgcc tacggcccct gcctgaaagc cgccaatggt aaggagtgta aagagaatta
     1921 ctacctgatg ccgggcaagt cgcgtcgttt tacccgcgtg gacacggcgg ataacaaagg
     1981 acgggttgca ctttggcagg gtgataagtt cattcccgtg aaatagatag ctgtgcagat
     2041 ggataacgac aatgccttta cgacggttct ccccaggact gaaagcccag tttgccttcg
     2101 gcatggtctt tttgttcgtt cagcccgatg ccagcgctgc tgacataagt gcgcagcaaa
     2161 taggtggggt gattattccg caggccttca gtcaggcgct ccaggacggc atgagcgtcc
     2221 cgctttatat tcatctcgcc ggtagccagg gtcgccagga cgatcagcga atcggcagcg
     2281 cttttatctg gttggatgat ggacagttac gcatccgaaa aatacagctg gaagagagtg
     2341 aagataacgc cagtgtcagc gaacaaactc gacagcagct gatgactctg gccaacgccc
     2401 cgttcaatga ggcccttacc atccccctga ctgacaacgc gcagttggat ctcagcttgc
     2461 gccaactgct gctgcagctg gtggtcaagc gcgaagcgct gggcaccgta ctacgctcac
     2521 gtagcgaaga catcgggcag tccagtgtta acaccctcag cagtaatctg agctataact
     2581 tcggcgtcta taacaaccag ttgcgtaacg gcgggagcaa cacctccagc tatctgtcgc
     2641 tgaataacgt tactgcgctg cgcgaacatc acgtggtgct cgacggctca ctgtacggga
     2701 ttggtagcgg tcaacaggac agtgaattat ataaagcgat gtatgaacgc gattttgctg
     2761 gtcaccgatt tgccggtgga atgctcgaca cctggaactt gcagtcctta gggccgatga
     2821 ccgccatttc agcagggaag atttacggcc tttcctgggg aaaccaggcc agctccacca
     2881 tcttcgacag cagccagtcg gccacgccag tgatcgcctt tttaccggcg gcgggcgaag
     2941 tacatctcac ccgtgatggg cggctactaa gcgttcagaa cttcaccatg ggcaatcatg
     3001 aagtggatac ccggggtcta ccgtacggta tttacgatgt ggaagttgag gtaatcgtta
     3061 acggtcgcgt gatcagcaaa cgcacccagc gggtcaataa gctgtttagc cggggacgtg
     3121 gcgtcggtgc accgctggcg tggcagatat ggggcggtag ctttcatatg gatcgctggt
     3181 cggaaaacgg gaaaaagacg cgaccagcca aagagagttg gctggcaggt gcctcgacct
     3241 ccggctcact gagtacgttt agctgggcgg caacgggata tggatacgat aatcaggcgg
     3301 tgggtgaaac ccgtctgacg ctgccgcttg ggggggcgat caacgttaac ctgcaaaaca
     3361 tgctggccag tgacagctca tggagcaaca tcgccagcat cagcgccact ctacctggag
     3421 gcttcagttc gctgtgggtt aaccaggaaa aaacccgcat tggcaatcaa ttgcgacgta
     3481 gcgatgccga caaccgtgca atcggcggca cactcaacct gaactcactg tggtcgaagc
     3541 tgggtacgtt cagcatcagc tacaatgatg accgccgcta caacagccat tattacacgg
     3601 cagattacta tcaaagtgtc tacagcggta cctttggttc gcttggcctg cgggccggta
     3661 ttcagcgcta taacaacggc gacagcagcg ccaatacagg gaaatatatc gctctcgatc
     3721 tctcgctacc actgggcaac tggtttagcg cagggatgac ccatcaaaac ggctacacca
     3781 tggcaaacct gtcagcacgc aaacagtttg atgaaggaac cattcgcact gttggtgcca
     3841 atctgtcacg agccatctcc ggcgataccg gtgatgacaa aaccctcagc ggtggggcgt
     3901 atgcacagtt cgacgctcgt tacgccagcg gaacgctgaa cgtcaatagc gcggcggacg
     3961 gctacatcaa tactaatttg accgccaacg gcagcgtcgg ctggcagggt aaaaacatcg
     4021 ctgccagcgg gcggaccgat ggcaacgctg gggtgatatt cgacactggc ctggagaacg
     4081 atggtcagat cagcgccaaa atcaacgggc ggattttccc gcttaacggc aagcgtaact
     4141 atctcccgct ctctccctat ggaagatatg aggtggagtt gcagaacagc aaaaactcac
     4201 tcgacagtta cgatatcgtc agcggtcgca aaagtcatct gactctctat ccaggcaatg
     4261 tcgctgtcat tgagccagag gtgaagcaga tggttaccgt ctccggtcgt atccgtgcgg
     4321 aagacggcac actgctggct aacgcacgga ttaacaacca tatcggccga acccgaaccg
     4381 atgaaaacgg cgagtttgtc atggacgtgg ataagaaata ccccactatc gattttcgct
     4441 acagtggcaa taaaacctgc gaagtggcac tggaactcaa ccaggcgcgc ggtgccgtct
     4501 gggtcggtga tgtggtctgc agcggcctct catcgtgggc ggcggtgacg cagacaggag
     4561 aagagaatga gagttaacct actgatagcg atgataatct ttgcgctaat ctggcctgca
     4621 accgcgctca gagcggcagt gagcaaaaca acctgggcgg atgctccggc acgcgagttt
     4681 gtgtttgtcg aaaacaactc agacgacaac tttttcgtca ctcctggcgg ggcgctggat
     4741 ccgcgcctga ccggtgccaa ccgctggacc ggtttaaaat acaatggttc aggaaccatc
     4801 tatcagcaaa gcctcggcta cattgataac ggttacaaca ccggccttta taccaactgg
     4861 aagtttgata tgtggctgga aaattcacca gtttcatctc ctttaactgg cttgcgctgc
     4921 atcaactggt acgcagggtg taatatgacc accagtctta tcctgccgca aaccaccgac
     4981 gccagtggat tttatggcgc gacggtgacc agcggcggcg cgaaatggat gcacggcatg
     5041 ttgtcagacg cgttttacca gtatctgcaa caaatgcccg tcggcagcag ctttacaatg
     5101 accatcaatg cctgccagac ctctgtgaac tatgacgcca gcagcggcgc acgctgtaag
     5161 gatcaggcct ccggcaactg gtatgttcgc aacgtcaccc atacgaaagc agcaaatctg
     5221 cggttgataa atacccactc gctggcggaa gtatttatca acagcgacgg agtaccgact
     5281 ctgggcgaag ggaacgccga ctgccggacg caaaccatcg gcagccgttc aggattaagt
     5341 tgtaagatgg ttaactatac cctgcaaaca aacggactca gcaacacctc aatccatata
     5401 ttcccggcga tcgccaactc gtcgttagcc tcggccgtcg gggcgtacga tatgcagttc
     5461 agtctgaatg gcagttcatg gaaaccggtg agcaatactg cctattacta caccttcaac
     5521 gagatgaaga gcgcagactc gatctatgtt ttcttctcga gcaacttctt taaacagatg
     5581 gtgaacctcg ggatcagcga tatcaacacc aaagatctat tcaactttcg ctttcagaac
     5641 accacatcac cggagtctgg ctggtatgaa ttttctacct ccaacacgct gattatcaaa
     5701 ccccgtgatt tcagcatcag tattatctcc gatgaatata ctcagacacc gtcgcgggag
     5761 ggatatgttg gcagcggcga gtcggcactc gatttcggct atatcgtaac caccagcggt
     5821 aaaacagctg ccgacgaagt gctgatcaag gtgaccggac ccgcgcaggt gattggcggg
     5881 cgctcctatt gtgtcttcag ctccgatgac ggtaaggcga aagtaccgtt cccggcgacg
     5941 ctttccttta ttacccgcaa cggagctaca aaaacctacg atgccgggtg cgatgatagc
     6001 tggcgggata tgaccgatgc gctgtggttg accacaccgt ggactgatat ctctggcgaa
     6061 gtggggcaga tggataagac cacagtcaaa ttttcgattc caatggataa cgccatttct
     6121 ctgcgtacgg tagatgataa cggctggttt ggcgaagtca gcgcttcagg agagattcat
     6181 gttcaggcga cgtggcgtaa cattaactaa ggccctgctg acagtggtct gtatgctggc
     6241 ggcacctttg acacaggcga tttcggtcgg caatctgact ttttcactgc cgtccgagac
     6301 tgactttgtc agcaaacgtg tcgtgaataa caacaaaagc gcgcggatat accgtattgc
     6361 catcagtgct attgatagcc cgggcagcag tgaattgcgc acccgaccgg tggatggtga
     6421 actacttttc gccccccgcc agctggcgtt gcagactggt gagagcgagt attttaaatt
     6481 ttactatcat ggtccacggg ataaccgcga gcgctactac cgggtctcat ttcgcgaggt
     6541 ccccactcgt aacctgacaa aacgcagccc aaccggcggt gaggtcagca cggagccggt
     6601 ggtggtgatg gataccattc tggtagtacg accacgtcag gttcagttta aatggtcctt
     6661 cgatcaggtg accggaacgg taagtaacac cggcaacacg tggtttaagc tactgattaa
     6721 accaggatgt gattcgaccg aagaggaagg cgatgcctgg tatctacgtc cgggagacgt
     6781 ggttcatcag cctgagttac gtcagccggg gaatcattat ctggtctata acgacaaatt
     6841 cattaagatt agcgattcct gtccggctaa gcccccttcg gcggactaag acttatccat
     6901 cggacgaggg aagaattcca cctcagagac tcaaaaatcg tttaaatgat gtggaagcga
     6961 tcgtcaatgt cacctgtaac gatgagcttt ctcatatgca caccacagtg ttcaggagac
     7021 acggaaaggt accaggcggc ctgctaacga gtggtttctt cactaattag gacgtaaaat
     7081 cggtaacggc tggaaatcat tcaatactca cactatcgaa agtacaccag ccaaccgcga
     7141 tacatccctg catacgacgt accgcagttc tctttacgat ttatagctgt actggtgaat
     7201 tatgagcaat ctgaatccat gcatgacgtg tggtgcctgt tgtgcatttt tccgcgtctc
     7261 tttttactgg gccgaagctg acgatgctgg cggtaccatt cccgccaggc tcactgaaca
     7321 aatatcccct tttcaccgat gcatgagcgg tactaatcag aaaaatcccc gatgtattgc
     7381 ccttgcagga acaccgggca aaaatgcctg ctgcacgata tatgaaaatc gatcgtccac
     7441 atgcagagaa ttcgccatgt ctggtgagaa cggagaagtc aatgaggctt gcaatcgtgc
     7501 aagagctaaa tacgggctaa caccgttata aatatacaat aattaattgc attgccccgc
     7561 cagctgtaat agcggggctt cgtttttcag ggacaacaaa acctgaaact tcttccattt
     7621 tccagcaaca atgcctcttc ttctgtagcg ttctccggat ctacatcatc ctgaagtagc
     7681 gcgatatgat cactgactgt ccgcatcaga atatgaaaag caacgttttt aacctataaa
     7741 aatggcgcta tatttgcgcc atttttatca ttcaaagcat tatctatttg agcctaaagg
     7801 gatctcagga tctggctcat gggtaattct gtttcgtaaa tctctgcgaa taatttcaat
     7861 agaccagaac cagactaaat gtccaaaaat ttcagagaca ttctcatacc acggaagatc
     7921 aaagagaggt ggcgtaagtc ccatgagagg gaatgaaatc atatgaacaa aaagttgcgc
     7981 taaagcacct gccagtaagc cctgccaaag tttaattttc ggaaatactt cggcgaccac
     8041 acagtaacca acggcaaaaa ctatcgaaaa gattatgtgc gttacaccaa cccagttaaa
     8101 gacatgcccg gcgaaggtat aaacagccga atttggatct gtcagtccca accaatcccg
     8161 aagaaaaatg tacggaggat tgagaaagtt acgggagcaa tcaatttggc ctgcagccct
     8221 gattaatgat tccggaccac acgctgcatt aaacatatca accggactac gtggtggcaa
     8281 tggaacttct gccccccact taacaaatgc ggaaacaacg ccagcaatca gcccaatgaa
     8341 tgcagcaaga ccataacgtc tgcggtttgg tggagtttgt tcaaatatat tcatatct
//
LOCUS       DAZFLD010000195         8335 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.196, whole genome shotgun sequence.
ACCESSION   DAZFLD010000195 DAZFLD010000000
VERSION     DAZFLD010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8335
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.196"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(87..293)
                     /locus_tag="ACHKBL_003965"
     CDS             complement(87..293)
                     /locus_tag="ACHKBL_003965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfA family protein"
                     /protein_id="HFO5820764.1"
                     /translation="MELYREYPAWLIFLRRTYAVAAGVLALPFMLFWKDRARFYSYLH
                     RVWSKTSDKPVWMDQAEKATGDFY"
     gene            606..695
                     /gene="kdpF"
                     /locus_tag="ACHKBL_003966"
     CDS             606..695
                     /gene="kdpF"
                     /locus_tag="ACHKBL_003966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HFO5820765.1"
                     /translation="MSAGVIAGVLLVFLLLGYLVYALINAEAF"
     gene            695..2368
                     /gene="kdpA"
                     /locus_tag="ACHKBL_003967"
     CDS             695..2368
                     /gene="kdpA"
                     /locus_tag="ACHKBL_003967"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HFO5820766.1"
                     /translation="MAAQGFLLIATFLLVLMVLARPLGSGLARLINDIPLPGTTGVER
                     VLFRALGVSDREMNWKQYLSAILGLNILGLAVLFFMLLGQHYLPLNPQQLPGLSWDLA
                     LNTAVSFVTNTNWQSYSGETTLSYFSQMAGLTVQNFLSAASGIAVIFALIRAFTRQSM
                     STLGNAWVDLLRITLWVLVPVALLIALFFIQQGALQNFLPYQAVNTVEGAQQLLPMGP
                     VASQEAIKMLGTNGGGFFNANSSHPFENPTALTNFVQMLAIFLIPTALCFAFGEVAGD
                     RRQGRMLLWAMSVIFIICTGVVMWAEVQGNPHLLALGADSSINMEGKESRFGVLVSSL
                     FAVVTTAASCGAVIAMHDSFTALGGMVPMWLMQIGEVVFGGVGSGLYGMMLFVLLAVF
                     IAGLMIGRTPEYLGKKIDVREMKLTALAILVTPTLALMGAALAMMTDAGRSAMLNPGP
                     HGFSEVLYAVSSAANNNGSAFAGLSANSPFWNCLLAFCMFVGRFGVIIPVMAIAGSLV
                     SKKSQPASSGTLPTHGPLFVGLLIGTVLLVGALTFIPALALGPVAEYLS"
     gene            2391..4439
                     /gene="kdpB"
                     /locus_tag="ACHKBL_003968"
     CDS             2391..4439
                     /gene="kdpB"
                     /locus_tag="ACHKBL_003968"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HFO5820767.1"
                     /translation="MSRKQLALFEPTLVVQALKEAVKKLNPQAQWRNPVMFIVWIGSL
                     LTTFISIAMASGAMPGNALFSAAISGWLWVTVLFANFAEALAEGRSKAQANSLKGVKK
                     TAFARKLREPKYGAAADKVPADQLRKGDIVLVEAGDIIPCDGEVIEGGASVDESAITG
                     ESAPVIRESGGDFASVTGGTRILSDWLVIECSVNPGETFLDRMIAMVEGAQRRKTPNE
                     IALTILLIALTIVFLLATATLWPFSAWGGNAVSVTVLVALLVCLIPTTIGGLLSAIGV
                     AGMSRMLGANVIATSGRAVEAAGDVDVLLLDKTGTITLGNRQASEFIPAQGVEEKTLA
                     DAAQLASLADETPEGRSIVILAKQRFNLRERDVQSLHATFVPFTAQSRMSGINIDNRM
                     IRKGSVDAIRRHVEANGGHFPVDVDQKVDQVARQGATPLVVVEGSRVLGVIALKDIVK
                     GGIKERFAQLRKMGIKTVMITGDNRLTAAAIAAEAGVDDFLAEATPEAKLALIRQYQA
                     EGRLVAMTGDGTNDAPALAQADVAVAMNSGTQAAKEAGNMVDLDSNPTKLIEVVHIGK
                     QMLMTRGSLTTFSIANDVAKYFAIIPAAFAATYPQLNALNIMRLHSPDSAILSAVIFN
                     ALIIVFLIPLALKGVSYKPLTASAMLRRNLWIYGLGGLLVPFIGIKVIDLLLTVCGLV
                     "
     gene            4448..5020
                     /gene="kdpC"
                     /locus_tag="ACHKBL_003969"
     CDS             4448..5020
                     /gene="kdpC"
                     /locus_tag="ACHKBL_003969"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HFO5820768.1"
                     /translation="MRGLRPALSTFLFLLLITGGVYPLLTTALGQWWFPWQANGSLIR
                     EGDTVRGSALIGQNFTGNGYFHGRPSATAEMPYNPQASGGSNLAVSNPELDKQIAARV
                     AALRAANPDASANVPVELVTASASGLDNNITPQAAAWQIPRVAKARNLSVEQLTQLIA
                     KYSQQPLVKYIGQPVVNIVELNLALDKLDE"
     gene            5013..7697
                     /gene="kdpD"
                     /locus_tag="ACHKBL_003970"
     CDS             5013..7697
                     /gene="kdpD"
                     /locus_tag="ACHKBL_003970"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     KdpD"
                     /protein_id="HFO5820769.1"
                     /translation="MNNEPLRPDPDRLLEQTAAPHRGKLKVFFGACAGVGKTWAMLAE
                     AQRLRAQGLDIVVGVVETHGRKDTAAMLEGLAVLPLKRQAYRGRHISEFDLDAALARR
                     PALILMDELAHSNAPGSRHPKRWQDIEELLEAGIDVFTTVNVQHLESLNDVVSGVTGI
                     QVRETVPDPFFDAADDVVLVDLPPDDLRQRLKEGKVYIAGQAERAIEHFFRKGNLIAL
                     RELALRRTADRVDEQMRAWRGHPGEEKVWHTRDAILLCIGHNTGSEKLVRAAARLASR
                     LGSVWHAVYVETPALHRLPEKKRRAILSALRLAQELGAETATLSDPAEEKAVVRYARE
                     HNLGKIILGRPASRRWWRRETFADRLARIAPDLDQVLVALDEPPARTINNAPDSRSFK
                     DKWRVQIQGCVVAAALCAVITLIAMQWLMAFDAANLVMLYLLGVVVVALFYGRWPSVV
                     ATVINVVSFDLFFIAPRGTLAVSDVQYLLTFAVMLTVGLVIGNLTAGVRYQARVARYR
                     EQRTRHLYEMSKALAVGRSSQDIAATSEQFIASTFHARSQVLLPDDNGKLQPLTHPQG
                     MTPWDDAIAQWSFDKGLPAGAGTDTLPGVPYQILPLKSGEKTYGLVVVEPGNLRQLMI
                     PEQQRLLETFTLLVANALERLTLTASEEQARMASEREQIRNALLAALSHDLRTPLTVL
                     FGQAEILTLDLASEGSPHARQASEIRQHVLNTTRLVNNLLDMARIQSGGFNLKKEWLT
                     LEEVVGSALQMLEPGLSSPINLSLPEPLTLIHVDGPLFERVLINLLENAVKYAGAQAE
                     IGIDAHVEGEKLQLDVWDNGPGLPPGQEQTIFDKFARGNKESAVPGVGLGLAICRAIV
                     DVHGGTITAFNRPEGGACFRVTLPQQTAPELEEFHEDM"
     gene            7694..>8335
                     /gene="kdpE"
                     /locus_tag="ACHKBL_003971"
     CDS             7694..>8335
                     /gene="kdpE"
                     /locus_tag="ACHKBL_003971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
                     /protein_id="HFO5820770.1"
                     /translation="MTNVLIVEDEQAIRRFLRTALEGDGMRVFEAETLQRGLLEAATR
                     KPDLIILDLGLPDGDGIEFIRDLRQWSAVPVIVLSARSEESDKIAALDAGADDYLSKP
                     FGIGELQARLRVALRRHSATAAPDPLVKFSDVTVDLAARVIHRGEEEVHLTPIEFRLL
                     AVLLNNAGKVLTQRQLLNQVWGPNAVEHSHYLRIYMGHLRQKLEQDPARPRHFI"
BASE COUNT         1722 a         2178 c         2447 g         1988 t
ORIGIN      
        1 taaatattcc tctgaacatc ttcctgatac aaaaaaaacc gccagcgttt ccgctggcgg
       61 ttttcaatgt attcagaact cagcaatcag taaaaatcac cagtcgcctt ttcggcctga
      121 tccatccaca ccggtttatc gctggttttc gaccagacgc ggtgcaggta gctgtaaaag
      181 cgggcgcggt ctttccagaa aagcatgaaa ggcagcgcca gaacgcccgc tgcaaccgca
      241 taagtacggc gtaaaaagat aagccatgca ggatattctc tgtagagttc catacttttc
      301 tccccctttt gtatctaccc ggtgaatagc accggaaaaa tgaatttgtt tatctgatga
      361 aaatagtacc gccttttgtg taattttact actcatccga ccacttattt ttgcttattg
      421 atggtttatt tacattcata ccgtaattaa gttacacaaa ggttaaatta atactaaaca
      481 ttagttaaat catggctttt gccattttta tacttttttt acaccccgcc cgcagatttt
      541 tgcgaaatct ttgcagccag aattctactc ttccggtatc acttttaggc cactggaggt
      601 gcactgtgag tgcaggcgtg atagccggcg tgttgctggt gtttttatta ctgggttatc
      661 tggtttatgc cctgatcaat gcggaggcgt tctgatggct gcgcaagggt tcttactgat
      721 tgccacgttt ttactggtgt taatggtgct ggcgcgtcct ttaggcagcg ggctggcgcg
      781 gctgattaat gacattcctc ttcccggtac gacgggcgtt gagcgcgtac tttttcgcgc
      841 gcttggcgtc tctgaccgcg agatgaactg gaagcagtat ctctcagcca ttcttggcct
      901 gaatatactg gggctggcgg tgctgttttt tatgttgctc ggtcagcact atctgccgct
      961 taatccacag cagttgccag gactgtcgtg ggatctggcg ctgaataccg ccgtcagctt
     1021 tgtcacaaat accaactggc aatcttacag cggtgaaacc acgttgagct atttcagcca
     1081 gatggcgggc ttaacggtgc aaaactttct ttctgccgcc agcgggattg cggtgatttt
     1141 tgccctcata cgtgcattta cccgccagag catgagcaca ctcgggaatg cctgggtcga
     1201 tctgctacgc atcacgttat gggtgctagt ccctgtggcg ttgttgatcg cattgttttt
     1261 tattcaacaa ggtgcgctgc aaaactttct gccctatcag gcggtgaata ccgttgaagg
     1321 agcgcaacag ctgttaccca tggggcctgt agcttctcag gaagcgatca agatgctcgg
     1381 cactaacggc ggtggcttct ttaatgccaa ctcgtcgcat ccgtttgaaa acccaaccgc
     1441 actgaccaac ttcgtgcaga tgctggcgat cttcttgatc ccaacagcgc tgtgctttgc
     1501 ctttggcgaa gtggcgggcg atcgccgtca ggggcgcatg ttgctgtggg cgatgtcagt
     1561 gatttttatc atctgcacag gcgtggtgat gtgggcagaa gttcagggta atccgcatct
     1621 gctggcactg ggcgcggaca gcagcatcaa tatggaaggt aaagagagtc gtttcggcgt
     1681 gctggtcagt agcctgtttg cggtcgtgac tacggcggct tcctgtggcg cggtgattgc
     1741 gatgcatgat tcgtttaccg ctcttggtgg catggtgccg atgtggctga tgcaaattgg
     1801 tgaagtggtg ttcggcggtg tcggttctgg tctttacggc atgatgctgt ttgtcctgct
     1861 ggcggtgttt attgctgggc tgatgattgg tcgtacaccg gaatatctgg gtaaaaaaat
     1921 cgacgtacgc gagatgaaac tgaccgcgct ggcgattctg gtcaccccaa cgctggcgct
     1981 gatgggcgcg gcgctggcaa tgatgaccga cgccggacgt agcgccatgc tcaaccctgg
     2041 cccgcatggt tttagcgaag tgctgtacgc cgtgtcatcc gccgctaaca acaacggcag
     2101 cgcctttgcc ggattaagcg ccaactctcc attctggaac tgtttgctgg cgttctgcat
     2161 gtttgtcggt cgcttcgggg taattatccc ggtgatggca attgccggtt cgctggtgag
     2221 taaaaagagc cagcccgcca gctccggcac gctgccaacg cacggcccgc tgtttgttgg
     2281 cctgttaatc ggcaccgtgt tgctggttgg cgcactgacc tttatccctg ccctggcgct
     2341 tggtccggtg gcggaatatc tctcctgatg atattgagtg agcactgaat atgagtcgta
     2401 aacaactggc gctattcgaa ccaacacttg tcgttcaggc gctgaaagaa gcggtgaaaa
     2461 aattaaaccc gcaggcgcaa tggcgtaatc cagtgatgtt tatcgtctgg atcggcagtc
     2521 tgttaaccac ctttattagc attgcaatgg caagcggcgc gatgcctggc aatgcgctgt
     2581 ttagcgcggc aattagcggc tggctgtggg tcaccgtact gttcgctaat ttcgccgagg
     2641 cgctggcaga aggccgcagt aaagcgcaag ccaatagtct gaaaggggtg aaaaaaactg
     2701 cctttgcccg caagctgcgt gagccgaaat atggcgctgc ggcggacaaa gttcccgcag
     2761 atcaactgcg taaaggcgat atcgtactgg tagaagctgg cgatattatc ccctgcgatg
     2821 gtgaagtcat tgaaggcggc gcatcggtcg atgaaagcgc catcaccggg gaatcggctc
     2881 cggtgatccg tgaatccggc ggcgattttg cctccgtcac tggcggcacg cgtattcttt
     2941 ctgactggct ggtgattgag tgcagcgtta accccggcga gacatttctg gatcggatga
     3001 tcgcgatggt ggaaggcgcg cagcgacgca aaacgccgaa cgagattgcc ctgaccatcc
     3061 tgctgattgc cctgactatc gtctttttac tggcaaccgc cacgctgtgg ccgttttccg
     3121 catggggtgg taatgcagtc agcgtaacgg tactggtggc gctgttggtc tgtctgatcc
     3181 caaccactat tggcggcctg ttgtcagcga tcggcgtcgc cgggatgagc cggatgttgg
     3241 gcgcgaatgt gattgccacc agcgggcgtg cagttgaagc ggcgggcgac gtggacgtcc
     3301 tgctgctgga taaaactggc accatcaccc tcggtaaccg tcaggcatca gagtttatcc
     3361 ctgcgcaggg cgtagaggaa aaaacgctgg ccgacgcagc acaactggct tcgctggccg
     3421 acgaaacgcc ggaaggccgc agtattgtga tcctcgccaa gcagcgcttt aacctgcgcg
     3481 agcgcgatgt gcagtcgctt cacgccactt ttgtaccgtt tactgcgcaa agtcgaatga
     3541 gcgggatcaa tatcgacaac cgcatgatcc gtaaaggttc tgtcgatgcc attcgccgcc
     3601 atgttgaggc taacggtggt cacttccctg tcgatgttga tcaaaaagtc gatcaggttg
     3661 cgcgtcaggg ggccacgccg ctggtggtgg tggaaggttc tcgtgtgctg ggcgttattg
     3721 cgctgaaaga tatcgtcaaa ggcggtatta aagagcgctt cgcccagctg cgcaaaatgg
     3781 gcattaaaac ggtgatgatt accggcgata accgtctgac tgccgccgcc attgctgcgg
     3841 aagcgggtgt cgatgatttt ctcgccgaag cgacaccgga ggccaagctg gcgttaattc
     3901 gtcagtatca ggcggaaggc cgtttggtgg cgatgaccgg cgacggcacc aatgatgctc
     3961 cggcgctggc gcaggcagat gtcgcggtag cgatgaactc cggtactcag gcggcgaaag
     4021 aagcgggcaa tatggtggat ctcgactcta acccgaccaa gttgatcgag gtggtgcata
     4081 ttggcaaaca gatgctgatg acccgtggct cgctgaccac cttcagcatt gccaacgatg
     4141 tggcgaaata cttcgccatt attccggcgg cgttcgcggc aacgtatccg cagttaaatg
     4201 cgctgaacat catgcgcctg cattcgcccg actccgccat tctcagtgcg gtcattttca
     4261 acgccttgat tatcgtcttt ttgattcccc tggcgttaaa aggcgtgagt tataaaccgc
     4321 ttaccgcttc tgccatgttg cgccgtaact tatggattta cggtctgggt gggctgctgg
     4381 tgccgtttat tggtatcaaa gtcattgatt tactgctgac cgtttgcggt ctggtgtgag
     4441 gtttaccatg cgtggattac gtccggcatt atcaacattt ctctttttgt tactgattac
     4501 tggcggtgtt tacccgctgc tgaccaccgc gctggggcaa tggtggtttc cctggcaggc
     4561 caacggttcg ttgattcgcg aaggtgatac ggtgcgcggc tcggcattga tcgggcagaa
     4621 ttttactggc aacggctatt ttcatggtcg cccgtcggca acggcagaaa tgccctataa
     4681 tccacaggct tctggcggga gcaatctggc ggttagtaac ccggagctgg ataaacaaat
     4741 cgctgcacgc gttgccgcat tacgggccgc taacccggat gccagcgcga acgttccggt
     4801 tgaactggtg acggcgtcgg caagcgggct ggacaataac atcaccccgc aagcggcggc
     4861 ctggcagatc ccacgcgtgg cgaaagcgcg taatctcagc gttgaacagc tcacgcagct
     4921 gatcgcaaaa tatagccaac aaccgctggt gaaatatatc ggccagccgg tcgtcaacat
     4981 tgtcgaactc aatctggcgc tggataagct tgatgaataa cgaaccctta cgtcccgacc
     5041 ccgatcgtct gctggaacaa actgccgcgc cgcatcgggg gaagctgaaa gttttcttcg
     5101 gtgcctgcgc aggcgtcggc aagacctggg caatgctggc agaagcccag cgactgcggg
     5161 cgcaagggct ggatattgtg gttggcgtgg tagaaaccca cgggcggaaa gataccgccg
     5221 caatgctgga agggctggct gttctgccgt taaaacgcca ggcgtaccgt gggcggcaca
     5281 tcagcgagtt tgatctcgat gccgccctcg cccgccgtcc ggcactgatc ttaatggacg
     5341 aactggcgca cagtaatgcg ccaggttctc gtcatcccaa acgctggcag gacatcgaag
     5401 agctgctgga agctggcatt gatgttttca ctaccgtcaa cgttcaacat ctggaaagcc
     5461 tgaatgatgt ggtcagcggc gtcaccggaa ttcaggttcg ggaaaccgtg cccgatcctt
     5521 tttttgatgc cgccgacgac gtagtgctgg tggacttgcc cccggacgat ctgcgccagc
     5581 gactgaaaga aggcaaagtc tatattgccg ggcaggcgga gcgcgccatt gaacattttt
     5641 tccgcaaagg caatctgatc gccctgcgcg aactggcact gcgccgtact gccgatcgcg
     5701 tagatgagca aatgcgcgcc tggcgggggc atcctggcga agagaaagtc tggcacacgc
     5761 gcgacgcgat ccttttatgc atcggccata acactggtag tgagaaactg gtccgcgcgg
     5821 cggcgcggct ggcgtcacgg ctgggtagcg tctggcacgc ggtgtatgtt gaaacccctg
     5881 ccctgcatcg cttaccggag aaaaaacgtc gggcaattct cagcgcctta cgtctggcgc
     5941 aggagctggg cgcggagacg gcaacacttt ctgatccagc ggaagagaaa gcggtagtgc
     6001 gttacgcccg tgagcataat ctcggcaaga ttattctcgg tcgcccagcc tcgcgccgct
     6061 ggtggcgtcg ggaaacgttt gctgaccgac tggcgcgcat cgcccccgat ctcgatcagg
     6121 tgctggtcgc gcttgatgaa ccacccgccc gcacgattaa caacgcgccg gatagccgct
     6181 cttttaaaga caagtggcgg gtacagattc agggatgcgt ggttgccgcc gcgttatgcg
     6241 ccgttatcac cttaattgcc atgcagtggc tgatggcgtt tgatgccgcc aacctggtga
     6301 tgctgtatct acttggcgtg gtggtggtgg cgctatttta tggacgctgg ccttcggtgg
     6361 ttgctaccgt cattaatgtg gtgagtttcg atctcttttt tatcgcccca cgcggcacgc
     6421 tcgccgtctc tgatgtgcaa tatctgctga ccttcgcggt gatgttaacc gtcgggctgg
     6481 tgatcgggaa ccttactgct ggcgtgcgtt atcaggcgcg ggtagcccgt taccgcgaac
     6541 aacgcacacg gcacttatat gaaatgtcca aagctctggc tgtgggccgc agttcgcagg
     6601 atatcgccgc caccagcgaa cagtttattg cctccacgtt tcatgcccgt agccaggtgt
     6661 tgctgcctga tgacaacggt aaattgcagc cgttaacaca tccgcaagga atgacgccgt
     6721 gggacgatgc catcgcgcag tggagttttg ataaaggtct gcctgcgggc gcgggcaccg
     6781 acacgttacc cggtgtaccg taccagattt tgccgctaaa aagcggcgag aaaacctacg
     6841 gactggtggt ggtggaaccg gggaatctgc gccagttgat gatcccggaa cagcagcgcc
     6901 tgctggagac gtttacgctg ttagtcgcca atgcgcttga gcggctgacg ctgaccgcca
     6961 gcgaagaaca ggcgcggatg gcaagcgaac gtgaacagat ccgcaacgct ctgctggcgg
     7021 cgctttcgca tgatttacgc acgccgctca cggtgctgtt cggtcaggca gaaatcttaa
     7081 cgctcgatct ggcaagcgaa ggatcgcccc acgcccgcca ggccagcgag attcgtcagc
     7141 atgtgctgaa cacgacccga ctggtgaata atctgctcga tatggcgcga attcagtccg
     7201 gcggctttaa tttgaagaaa gagtggttaa cgctggaaga agtggtcggc agcgcgctgc
     7261 aaatgctgga accgggttta tcgtcgccta tcaatctttc tctgccagaa ccgctgacct
     7321 taatccacgt tgacgggccg ctctttgaac gggtgctgat taatctgctg gagaacgcgg
     7381 tgaaatacgc gggtgcgcag gccgaaattg gtatcgatgc ccacgttgag ggcgagaagc
     7441 tacaactgga tgtctgggat aacggccccg gtcttccgcc aggccaggag cagacgatat
     7501 ttgataagtt tgctcgcggg aataaagagt cggcggtacc gggtgtaggg cttgggctgg
     7561 caatttgtcg ggcgattgtg gatgtacacg gcggcaccat taccgcgttc aaccgaccgg
     7621 aaggtggtgc ctgttttcgt gttacacttc cccagcaaac cgctcctgaa cttgaagaat
     7681 ttcatgagga tatgtgacaa acgttctgat tgttgaagat gaacaggcta ttcgtcgctt
     7741 tctgcgcacg gcgctggaag gcgacggtat gcgcgtcttt gaggccgaaa cgctgcaacg
     7801 cggcttgctg gaagcggcta cccgtaagcc agatttgatt attctcgatc tcggcctgcc
     7861 cgatggtgat gggattgagt ttatccgcga cctgcgccag tggagcgcgg tgccggtgat
     7921 tgtgctttcc gcacgtagcg aagagagcga caaaatcgcc gcgctggatg ccggagcgga
     7981 tgattatctg agtaagccgt ttggcattgg cgaattgcag gcccgtctgc gcgtcgcttt
     8041 acgtcgccac tccgccaccg ccgcgcccga tccgctggtg aaattttccg atgttaccgt
     8101 cgatttagca gctcgtgtga tccatcgggg cgaggaagag gtgcatctca ccccgattga
     8161 gttccgcctg ctggcagtgt tgcttaataa tgcgggaaaa gtgctcaccc agcgccagct
     8221 ccttaaccag gtgtgggggc caaacgcggt cgaacacagt cattatttgc gtatttatat
     8281 ggggcatctg cgacaaaaac tggaacagga cccggcccgg cctcgccatt tcatt
//
LOCUS       DAZFLD010000196         8305 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.197, whole genome shotgun sequence.
ACCESSION   DAZFLD010000196 DAZFLD010000000
VERSION     DAZFLD010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8305
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.197"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(3..275)
                     /locus_tag="ACHKBL_003972"
     CDS             complement(3..275)
                     /locus_tag="ACHKBL_003972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309238.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF826 domain-containing protein"
                     /protein_id="HFO5820771.1"
                     /translation="MSEITSLVTAEAVKEVLRSEEVRSALKQKLRQNLEARLDAEVDA
                     ILDELLGVQAEPPTEAGDTTAESGEVQPESPVADATEPQPESVMML"
     gene            complement(301..483)
                     /locus_tag="ACHKBL_003973"
     CDS             complement(301..483)
                     /locus_tag="ACHKBL_003973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001304601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1378 family protein"
                     /protein_id="HFO5820772.1"
                     /translation="MSFIHQIMLYFCTAVCVLYLLSGGYRAVRDFWRRQIDKRAAEKI
                     SASQSAGSKPEEPVTP"
     gene            complement(630..2669)
                     /locus_tag="ACHKBL_003974"
     CDS             complement(630..2669)
                     /locus_tag="ACHKBL_003974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000874434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6645 domain-containing protein"
                     /protein_id="HFO5820773.1"
                     /translation="MAFKHYDVVRAASPSDLAERITQKLKEGWQPYGSALISTAGYGA
                     EFIQPVVSEGSISSPEEPGNRPTTSAPSVAPEYYYVIALAGQSNGMSYGEGLPLPDTF
                     DSPDPRIKQLARRSTVTPGGAACTYNDIIPADHCLHDVQDMSSLHHPNADLSKGQYGC
                     VGQGLHIAKKLLAFIPKNAGILLVPCCRGGSAFTSGAEGAFSESSGATAESSLWGVGK
                     PLYNDLLTRTKAALDKNPKNVLLSVVWMLGEFDLKSATYAQHPQLFLNMVNQFRADLS
                     AYSSQCTGGNASFVPWICGDTTYLWKQKYTSQYPVVYGAYQNKEAQKIYFVPLMTDEK
                     GTNTPTNLPAEDPDLAEAGYYGAASRSNGNWTSSDRATHFSSWARRGIVSDRLATAIL
                     MHAGKTAAFLKGTPAPFSVVVAHPATAGSDSATTESPQPSTSAPQPGAGTQPSPEVPP
                     ATETPARTTAVAYRADTQEGKLKEQGWTGADGKASVVDDSAAESGKAMRVEKVQGQQT
                     AWKISHDVPASKAAELLQRGGEIAFRFKIPEGVQLVTNRFAMALYWPVSQLPENVVLE
                     GSAGNDMLAAFFIQSDSANLNLMQHKQQNAKLGTFGAFNHNWHNVVFRFAGNNSISVT
                     PVINGQTQAAFNLAKCPAAGLDNDKLLLTDITSNQDTYPVLIEHVTVAINNSNAA"
     gene            complement(3432..3866)
                     /locus_tag="ACHKBL_003975"
     CDS             complement(3432..3866)
                     /locus_tag="ACHKBL_003975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HFO5820774.1"
                     /translation="MRDIRQVLERWGAWAANNYEDVTWSPIAAGFKGLIPEKVKSRSQ
                     CCDDDAMVICGCIARLYRNNRDLHDLLVDYYVLGETFMALARKHGCSDTCIGKRLHKA
                     EGIVEGMLMMLGVRLEMDRYVERELPGGRTSVFYQRKNSLRS"
     gene            complement(3859..4053)
                     /locus_tag="ACHKBL_003976"
     CDS             complement(3859..4053)
                     /locus_tag="ACHKBL_003976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage NinH family protein"
                     /protein_id="HFO5820775.1"
                     /translation="MTFTVKTIPDMLVEAYGNQTEVARILNCNRATVRKYIGDKEGKR
                     HAIVNGVLMVHRGWGKDTDA"
     gene            complement(4050..4613)
                     /locus_tag="ACHKBL_003977"
     CDS             complement(4050..4613)
                     /locus_tag="ACHKBL_003977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinG"
                     /protein_id="HFO5820776.1"
                     /translation="MQQRKPKKCKVCGTPFVPFRSYQKVCCGQCALELVRKEKAIASA
                     KEQADKLKARRRDLQPRSYWIKQAQQAVNAYIRERDRNLPCVSCGTFDSAQWDAGHYR
                     TTAAAPQLRFDERNIHKQCVVCNQYKSGNLVPYRVELINRIGQEAVDEIESNHNRHRW
                     TVEECRAIKAKYQQKLKDLRNSRSEAA"
     gene            complement(4621..5070)
                     /locus_tag="ACHKBL_003978"
     CDS             complement(4621..5070)
                     /locus_tag="ACHKBL_003978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HFO5820777.1"
                     /translation="MEIEMIKAANGVFVPAYERDLPRLAKFKNGELYTLEAKLTRNPS
                     FHRKMFAFLNFCFQYWCAEHAGYEFSDEATQFDEFRKNLTILAGFYDVVTTIRGEVRY
                     RAKSLSYANMDQDEFERCYNAMINAALKHVFGRSNSPELNNRLLSFF"
     gene            complement(5070..6041)
                     /locus_tag="ACHKBL_003979"
     CDS             complement(5070..6041)
                     /locus_tag="ACHKBL_003979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001694414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primase-helicase zinc-binding domain-containing
                     protein"
                     /protein_id="HFO5820778.1"
                     /translation="MQTKKAAVGQWGRIFEYYGMPPVTGVKHYNGPCPICGARGKFRC
                     DDKDGSGSWICVCGHGDGMKLLQLATGKPWVTLCDEIDRLIGNTWKREKVSQPVTEIS
                     RKRKLVMDKFSRLSCLRGTTGEAYLQGRGILQLPTESVRFCDRQIASGREYQAIYAIA
                     TDDKGSLCYLHRTLLDGDRKANVEAAKKMTALQELPGLQHAKSVAIRLYPVSSTLGIA
                     EGIETALSCRQIFRCNVWSTMNSGFMEKFIAPPGVNHLIIFADNDEHGAGLAAAFKCG
                     HKNLMSRNDVEKVSIRWPDLPDFNDMLIQGCEAREHVLTRKFKAEAA"
     gene            complement(6031..7551)
                     /locus_tag="ACHKBL_003980"
     CDS             complement(6031..7551)
                     /locus_tag="ACHKBL_003980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFO5820779.1"
                     /translation="MLKITPNFAQERGLNQLRHQWKQHRTYLMYAPTGSGKTGLAAFV
                     TAGMVERGMRVMFVCPYTILLNQTAERFTEYGLPWEEISFVWRDHPNYDPSRLIQIAS
                     ADTLIRREFPDNIDLLIIDEAHMKRRALLEVIRDRDIRVLGLSGTPFAAWMGKYYECL
                     IKPTTIRELIQRGDLSDYEFFAPSMPDLAGVKTSNTVFGRDYNEEQLASIMGSSDLVG
                     DIVSNWLENGEDLPTICFCVNVAHANFVTREFLQSGIGAEVMTADTPHDERQDIIRRF
                     EEGATKIIVNVGVLVAGFDSDVRCLIYARPTKSEIRWLQCIGRALRTASGKKRALIFD
                     HSGTVHRLGFPEDIEYDELPGKNDGMKASAGGGEVKAEKLPRECPKCHFMKPAGVHMC
                     PKCGFRPLGGDDVATDRDRKLSRVNKGKREYSREEKQRWWSEIKGYQNYRNATGKPLS
                     DGWCAHTYKEKFGVWPKGFSNAPLQTSVEVHNFIRSKIIAYAKGRKKAMTGGQHAD"
     gene            complement(7545..7922)
                     /locus_tag="ACHKBL_003981"
     CDS             complement(7545..7922)
                     /locus_tag="ACHKBL_003981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5820780.1"
                     /translation="MSAAIKSTLIRTLSTVPLRTEDRYSFLADVVTILESQGMHVARD
                     VTVRIDGRNFRVDILATAKTGGSVAIEIDRSSPRPRSVMKLRELARRGTEGFVLLRMP
                     KKLTSYSDAGIEIIPANGKGASC"
     gene            complement(8089..>8305)
                     /locus_tag="ACHKBL_003982"
     CDS             complement(8089..>8305)
                     /locus_tag="ACHKBL_003982"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5820781.1"
                     /translation="RESVPGRVGQGACIVQEGQQGKTERQHPVTGVCPEVRESQPWLD
                     AFASSGTEVSPDTMMAVSCVRNNFTCN"
BASE COUNT         1921 a         2268 c         1930 g         2186 t
ORIGIN      
        1 cgttacaaca tcatgaccga ttcgggttga ggttcagtcg catcggcgac cggtgattca
       61 ggctgaactt caccgctctc tgcggtggta tctcccgctt cagtcggtgg ctctgcctgt
      121 acaccaagca gctcatccag aatggcatca acttctgcat caagacgcgc ctcaagattc
      181 tggcggagtt tctgtttcag tgcactccgg acttcttcag agcgcaggac ttccttcact
      241 gcctctgcag tgaccaggga tgtaatttct gacatgggat tttctcgctg aaaggggttg
      301 ttaaggagta acgggctctt cgggtttgct tccggctgac tgactggcgc tgattttctc
      361 agcggccctt ttatcaatct gcctgcgcca gaaatcgcgc actgccctgt acccacccga
      421 aagaagatac agcacacaga ccgccgtaca gaaatacagc atgatttgat gaatgaatga
      481 cataatcacc tcacacaaat gattgacata acgatgtctg gcgttttaaa aaaaatgaat
      541 aacaataata aacactctcc ataattcctc ttacgctccc ggctggtatc ccttcctccc
      601 agtcttgcct gccgggagcc tcttttttct tatgcggcgt tgctgttatt gattgccact
      661 gttacgtgct caatcagaac cgggtaggta tcctggttac tggtaatgtc agtgagtaac
      721 agcttgtcgt tatccagtcc tgccgccggg catttcgcca gattaaaggc cgcctgggtc
      781 tgaccgttga tgaccggtgt cacgctgata ctgttattac cggcaaaccg gaacaccacg
      841 ttgtgccagt tatgattaaa tgccccgaag gttcccagtt ttgcattctg ttgtttgtgc
      901 tgcatcagat tcaggtttgc gctgtcgctc tggatgaaga aggcggccag catatcgttt
      961 cctgcgctgc cttccagaac aacgttttca ggtagctgag aaacaggcca gtacagggcc
     1021 atggcgaagc ggtttgttac cagctgaaca ccttcgggaa ttttgaaacg gaaggcaatt
     1081 tcaccgcctc tctgaagtaa ttcagctgct ttacttgcag gcacatcgtg ggaaattttc
     1141 caggctgttt gttgcccctg gactttttcc actcgcatgg ctttaccgct ctcagcagca
     1201 gaatcatcta cgacagaggc ttttccgtct gcccccgtcc agccctgttc tttcagtttc
     1261 ccttcctgtg tgtctgcacg ataagcgacg gcagttgttc ttgccggtgt ttcagttgcc
     1321 ggtggaactt ccggtgatgg ctgtgtgcct gcgcccggct gaggtgcaga tgtggagggc
     1381 tgcgggcttt ccgtggtggc gctgtctgaa cctgccgtgg cgggatgcgc tacgaccacg
     1441 ctgaacggtg cgggtgtgcc tttcaggaag gcggctgtct tccctgcatg catcaggatc
     1501 gccgttgcca gacggtcaga aacaatgccg cgacgggccc atgaactgaa atgcgtggcg
     1561 cgatctgaag aggtccagtt tccgttactg cgggatgcag caccgtaata accggcttct
     1621 gccagatcag gatcttcggc aggcaggttt gttggcgtgt tagtaccttt ctcatcggtc
     1681 atcaggggca caaaataaat tttttgcgct tctttgttct gatatgcacc gtaaaccacc
     1741 gggtactgag aggtatattt ttgtttccag agataggtgg tatcgccgca aatccaggga
     1801 acaaatgatg cattgcctcc ggtgcactgt gaagaatatg cgctgagatc ggcgcggaac
     1861 tgattcacca tgttcaggaa taattgcggg tgctgtgcgt atgttgctga cttcagatca
     1921 aactctccca gcatccagac cacggataac aggacgtttt tggggttttt atccagcgcg
     1981 gcttttgtac gggtaagcag atcgttgtac agcggcttgc cgacacccca cagggatgat
     2041 tcggccgtcg ctcctgacga ttcactgaat gccccctcag cgccggaagt aaatgctgac
     2101 cctccacgac agcaaggtac cagaaggatc ccggcatttt tcgggataaa cgccagtaat
     2161 tttttggcga tatgtaaacc ctgtccgacg cagccatact gaccttttga cagatcggcg
     2221 ttcggatgat gaaggctgct catatcctgt acatcatgga gacagtgatc tgccggaata
     2281 atatcgttat aggtacatgc agcgccgccc ggtgtcaccg tactgcgacg cgctaactgt
     2341 ttaattcgtg gatcagggct gtcgaatgta tccggtaatg gcagtccctc accgtatgac
     2401 ataccattgg actgaccagc aagcgcgatc acatagtaat attctggcgc aacagaaggc
     2461 gctgaggtcg tcggacggtt gcctggctcc tctggtgatg agatgctccc ctcactcaca
     2521 actggctgga tgaactccgc accataacca gctgtcgaaa tcagcgcact accataaggc
     2581 tgccaccctt ccttcagttt ttgagttatt cgttccgcaa ggtctgacgg cgacgccgcc
     2641 ctgaccacgt catagtgttt aaatgccatg aatcctcccg gccgggataa tgtactgaat
     2701 aagataagga gtgagcggaa gtcccgaaac acaagggaaa aagacaacca cccgcaaaaa
     2761 agaaatacac tcctcacagt tgcgcagggt gattactgta aggtattatt cgcagcgtta
     2821 attaattgtt catattgctt ttcttcttgc caaccgctct tcccggggcg gctttttttt
     2881 gcatgtaaaa aggcccctgc tatgaggggc cctgatatat gcctaatctc tgtatactgc
     2941 atggtgccgg gtgcctcccg gtgagttcgg cctggtgcca ccaaacccgc gtattctcgc
     3001 ttacgatcat caaagagatc ataccattca ccagtcgccc ctccgcacag ggggattcac
     3061 catgcagttt ttttctaaca aattctcatc cgggcagaca acattcaaca gacttaattg
     3121 tgaggtatgt aacattcctg ttgaacggat acaaaaaaag ccagccactg ggggaggctg
     3181 gcaaactcgt agagcaaaat gctgttacgc aaacttcgtt acagggtcat cctgcaatac
     3241 ttaaaatata caatatttag aaaactaata gtgccatatg cgatttttaa gattttgtta
     3301 ttaattgcgg tcgcaccttc ctttctgtgt actttccgta taactaacag aattccggat
     3361 acaaaaaacc cgcgcatcgg cgggttaagc agcgtggcaa tgtaaccact cttatcatga
     3421 tatgcagatt tttacgatcg taaactattt tttcgctgat aaaatacaga ggttctccct
     3481 cccggcaatt cacgctcaac ataccgatcc atctcaagcc tcactcccag catcatcagc
     3541 atgccttcaa caatcccctc cgctttgtga aggcgtttac ctatacaggt gtcagagcac
     3601 ccatgtttcc gtgccagcgc catgaacgtc tcccccaaca cgtagtaatc aaccagcaag
     3661 tcatgcagat cgcgattgtt ccggtaaagg cgggctatgc acccgcatat caccatcgca
     3721 tcatcgtcac agcactgtga acgtgatttt actttttcgg ggatcagtcc cttaaatccg
     3781 gcagcaatgg gcgaccatgt aacatcctca tagttatttg ccgcccatgc cccccagcgc
     3841 tcaagaacct gccggatatc acgcatcagt atctttaccc catccgcggt gaaccataag
     3901 aacaccgttg acgatagcgt gtcttttccc ttctttatcg ccaatgtatt ttctgactgt
     3961 ggcacgattg cagttcagta ttcgggctac ctctgtctga tttccatatg cctcaacgag
     4021 catgtcagga atggttttta ctgtgaacgt catgcggcct cacttctgct gtttcgcagg
     4081 tctttaagtt tctgctgata cttcgccttg atcgccctgc attcttcgac agtccagcga
     4141 tggcggttat ggttcgattc gatttcgtct actgcttcct gcccgatgcg gttaatcagt
     4201 tcgacgcgat acggaacgag atttccgctt ttgtactggt tacacaccac gcattgcttg
     4261 tggatattgc gttcatcaaa tctgagttgg ggagccgcag ctgttgtacg gtaatgacct
     4321 gcatcccact gagcagaatc gaatgtcccg catgaaacgc acggcaaatt acggtctcgt
     4381 tccctgatat aagcattcac ggcctgctgt gcctgcttaa tccagtaact gcggggctgt
     4441 aagtcccttc ttcgcgcttt aagtttgtct gcctgttctt ttgcagaggc tatggccttt
     4501 tcttttctga ccagttcaag tgcgcactga ccgcaacaaa ctttctgata cgagcgaaac
     4561 ggcacaaacg gggttccgca cactttgcat tttttaggtt tacgttgctg catcatcacc
     4621 tcagaaaaac gacagcaggc ggttattcag ttcagggctg tttgagcgcc cgaacacatg
     4681 ttttaacgcg gcattaatca ttgcgttgta gcaacgttca aactcatcct gatccatatt
     4741 cgcgtaactc aggctttttg cccgatatct cacctcgcct cttatggttg tgaccacatc
     4801 atagaacccg gcaagaattg tcagattttt acgaaactca tcaaactgcg tcgcttcatc
     4861 agagaattcg tatccagcat gttcagcgca ccagtactga aagcagaaat taagaaaagc
     4921 gaacatcttc cggtgaaaag atgggttacg ggtaagtttt gcttccagtg tatacagctc
     4981 accgttttta aattttgcca gtcggggtaa atcgcgctca tacgccggta caaatacgcc
     5041 attagccgcc ttgatcatct ctatttccat caggcagcct ccgctttgaa tttgcgcgtc
     5101 aacacatgtt cacgggcttc acacccctga atgagcatgt cgttaaaatc cggcaagtca
     5161 ggccagcgaa tgctgacctt ctcaacgtca ttacgactca tgagattctt atgcccacat
     5221 ttaaaggcag ccgccagacc tgcgccgtgc tcatcattgt ctgcaaagat aatcaggtga
     5281 ttaacgcctg gtggcgcaat aaacttctcc ataaaaccgg agttcattgt tgaccacaca
     5341 ttgcagcgga agatttgacg gcatgaaagc gcagtttcga taccctcggc tatccccaga
     5401 gtggacgaca ccggatacag gcgtattgcc accgatttgg catgctgcaa accaggcaac
     5461 tcctgtagcg cggtcatttt tttagccgcc tctacattcg ccttgcgatc accatccagc
     5521 aacgtacgat gcagatagca aagagaacct ttgtcatctg ttgcaatggc gtaaattgcc
     5581 tgatattcgc gcccgctggc gatctgacgg tcacagaaac gcacggattc ggtcggtaac
     5641 tggagtattc ccctcccctg caggtacgcc tcacctgttg tgccccgcag acatgaaagt
     5701 ctggagaact tatccatgac cagcttccgc tttctgctta tctctgttac aggctggctg
     5761 actttctccc tcttccaggt atttccgatc agccgatcga tttcatcaca caacgtcacc
     5821 cagggcttac ccgtggcaag ttgcaacaac ttcatcccgt ccccgtgacc acagacgcaa
     5881 atccacgaac cggagccatc tttatcatca cagcgaaatt tacctctggc accacatatc
     5941 gggcatgggc cgttgtaatg tttcacacca gtaacaggag gcatgccgta atattcaaaa
     6001 attcgccccc attgcccgac agccgccttc ttagtctgca tgctggcctc cggtcatggc
     6061 tttcttgcgc cctttggcat aggcaatgat ctttgacctg atgaaattgt gcacttcaac
     6121 cgaggtttgc agcggcgcgt tactgaagcc tttaggccac accccgaact tctctttgta
     6181 ggtatgagcg caccatccgt cactcagtgg cttacctgtc gcgttgcggt aattctgata
     6241 gcccttgatc tcactccacc accgctgttt ttcctcgcgg ctgtattcgc gtttcccttt
     6301 gtttacgcga gagagttttc gatcgcggtc tgttgccaca tcatcgccac caagaggacg
     6361 aaacccacat ttgggacaca tgtgtacacc agcaggcttc atgaagtggc atttggggca
     6421 ttctctggga agtttttcag ctttaacctc gccaccgcca gcagacgctt tcattccgtc
     6481 atttttaccc ggcagttcgt catactcgat atcctcgggg aagcccagcc gatgaaccgt
     6541 gccggaatgg tcgaaaatca atgcgcgttt tttaccagac gcagtacgta acgctctccc
     6601 gatgcactgc aaccagcgga tttctgattt ggtcggacgg gcgtagatca ggcagcgaac
     6661 atcgctgtca aatcccgcaa ccagtacgcc aacgttgaca attattttgg tcgcaccctc
     6721 ttcaaaacgg cgaatgatgt cctgtcgttc atcatggggg gtatctgccg tcatcacctc
     6781 tgcgccaatg ccagactgta aaaactcacg ggtaacaaag ttcgcatgag ccacattcac
     6841 gcagaagcag atcgtcggta aatcctcgcc attctcaagc cagttgctga cgatatcgcc
     6901 aaccagatcc gaactcccca tgatggatgc gagctgttcc tcgttgtaat cccgtccaaa
     6961 tacggtgtta ctggttttta cgccagccag atcaggcatt gagggggcaa aaaactcata
     7021 atcgctcaga tcaccacgct gaataagttc acgaatcgtt gtaggcttga tcaggcattc
     7081 gtagtacttc cccatccagg ctgcgaacgg ggtaccggaa agtccaagaa ccctgatgtc
     7141 tctgtcccgg ataacctcaa gtaacgcacg acgcttcatg tgtgcttcat cgatgatcag
     7201 aagatcgatg ttgtcaggaa actcacgacg gatcagagtg tcggcactgg caatctgaat
     7261 cagtcgtgac gggtcatagt tagggtgatc acgccataca aaactgattt cttcccacgg
     7321 caaaccgtat tcggtgaaac gttccgctgt ctggttcagc aggatggtgt acggacagac
     7381 aaacatcact cgcataccac gttcaaccat tccggcggtg acgaatgctg caagaccagt
     7441 tttgccagag ccagtgggtg catacatcag gtacgtgcga tgctgtttcc actggtgccg
     7501 cagctggttt aaaccgcgct cctgagcaaa gtttggggtg attttcagca tgacgccccc
     7561 ttgccattcg ccgggatgat ctcgatccca gcatcactat aactggtcag tttctttggc
     7621 attcgaagca acacaaagcc ttcagtgcca cgacgtgcca attcacgcaa tttcatgact
     7681 gagcggggtc gcggagagct acgatcgatc tcaatagcca cactcccacc agtttttgct
     7741 gtcgcgagaa tatcaacacg gaaatttctg ccatcgattc ttactgtgac atctcttgcc
     7801 acatgcattc cctgtgactc aaggatcgtt acaacatcag ccagaaagct gtaacggtcc
     7861 tctgttctca gtggtacagt gctcagtgta cggatcagtg tcgattttat tgctgctgac
     7921 atagtttatc actcaataat tagcttgata attatttcac cagaaactat cgcgctctta
     7981 tacagtgatc tacctaacct atgtacctgc cttctggcct aggccaaggg atacaggcct
     8041 taccaacgac tccaccccct taccccctcc tcgcttcctt tcaggaaatt agttgcatgt
     8101 gaaattattt ctgacgcaac taaccgccat catggtgtcg ggggaaactt ccgtaccgct
     8161 actggcgaaa gcatccagcc acggctggct ctcgcgtact tctggacata cacccgtaac
     8221 cgggtgttgg cgctccgtct tgccctgttg cccttcctga acgatacagg ctccttgtcc
     8281 cactcttcct ggtacactct cgcgt
//
LOCUS       DAZFLD010000197         8167 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.198, whole genome shotgun sequence.
ACCESSION   DAZFLD010000197 DAZFLD010000000
VERSION     DAZFLD010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8167)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8167)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8167
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.198"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..2886
                     /gene="evgS"
                     /locus_tag="ACHKBL_003983"
     CDS             <1..2886
                     /gene="evgS"
                     /locus_tag="ACHKBL_003983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0010447 - response to acidic pH [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system histidine kinase EvgS"
                     /protein_id="HFO5820782.1"
                     /translation="PRQYNFLLTRKDSIVLNEVLNRFVDALTNEVRYEVSQNWLDTGN
                     LAFLNKPLELTEHEKQWIKQHPDLKVLENPYSPPYSMTDETGSVRGVMGDILNIITLQ
                     TGLNFSPITVSHNIHAGTQLNPGGWDILPAAIYSEDRENNVSFAEAFITTPYVFVMQK
                     APDSEQTLKKGMKVAIPYYYELHSQLKEMYPEVEWIKVDNASAAFHKVKEGELDALVA
                     TQLNSRYMIDHYYPNELYHFLIPGVQNASLSFAFPRGEPELKDIINKALNAIPPSEVL
                     RLTEKWIKMPNVTIDTWDLYSEQFYIVTTLSVLLVGSSLLWGFYLLRSVRRRKVIQGD
                     LENQISFRKALSDSLPNPTYVVNWQGNVISHNSAFEHYFTADYYKNAMLPLENSESPF
                     KDVFSNTHEVTAETKENRTIYTQVFEIDNGIEKRCINHWHTLCNLPASEHAVYICGWQ
                     DITETRDLIHALEVERNKAINATVAKSQFLATMSHEIRTPISSIMGFLELLSGSGLSK
                     EQRVEAISLAYATGQSLLGLIGEILDVDKIESGNYQLQPQWVDIPTLVQNTCHSFGAI
                     AASKSIALSCSSTLPERYLVKIDPQAFKQVLSNLLSNALKFTTEGAVKITTSLGHIDD
                     NHAVIKMTIMDSGSGLSQEEQQQLFKRYSQTSAGRQQTGSGLGLMICKELIKNMQGDL
                     SLESHPGIGTIFTITIPVEITQQVAAVEAKAEQPITLPEKLSILIADDHPTNRLLLKR
                     QLNLLGYDVDEATDGVQALHKVSMQHYDLLITDVNMPNMDGFELTRKLREQNSSLPIW
                     GLTANAQANEREKGLNCGMNLCLFKPLTLDVLKTHLSQLHQVAHIAPQYRHLDIEALK
                     NNTANDLQLMQEILMTFQHETHKDLPAAFHALEAGDNRTFHQCIHRIHGAANILNLQK
                     LINISHQLEITPVSDDSKPEILQLLNSVKEHIAELDQEITVFCQQND"
     gene            complement(2942..4087)
                     /gene="yfdE"
                     /locus_tag="ACHKBL_003984"
     CDS             complement(2942..4087)
                     /gene="yfdE"
                     /locus_tag="ACHKBL_003984"
                     /EC_number="2.8.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416872.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA:oxalate CoA-transferase"
                     /protein_id="HFO5820783.1"
                     /translation="MTNNECKGPFEGLLVIDMTHVLNGPFGTQLLCNMGARVIKVEPP
                     GHGDDTRTFGPYVDGQSLYYSFINHGKESVVLDLKNDHDKSIFINMLKQADVLAENFR
                     PGTMEKLGFSWERLQEINPRLIYASSSGFGHTGPLKDAPAYDTIIQAMSGIMMETGYP
                     DAPPVRVGTSLADLCGGVYLFSGIVSALYGREKSQRGAHVDIAMFDATLSFLEHGLMA
                     YIATGKSPQRLGNRHPYMAPFDVFDTQDKPITICCGNDKLFSALCQALELTELVNDPR
                     FSSNILRVQNQAILKQYIERTLKTQAAEVWLARIHEVGVPVAPLLSVAEAINLPQTQA
                     RNMLIEAGGIMMPGNPIKISGCADPHVMPGAATLDQHGEQIRQEFSS"
     gene            complement(4161..5105)
                     /gene="yfdV"
                     /locus_tag="ACHKBL_003985"
     CDS             complement(4161..5105)
                     /gene="yfdV"
                     /locus_tag="ACHKBL_003985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter YfdV"
                     /protein_id="HFO5820784.1"
                     /translation="MLTFFIGDLLPIIVIMLLGYFSGRRETFSEDQARAFNKLVLNYA
                     LPAALFVSITRANREMIFADTRLTLVSLVVIVGCFFFSWFGCYKFFKRTHAEAAVCAL
                     IAGSPTIGFLGFAVLDPIYGDSVSTGLVVAIISIIVNAITIPIGLYLLNPSSGADGKK
                     NSNLSALISAAKEPVVWAPVLATILVLVGVKIPAAWDPTFNLIAKANSGVAVFAAGLT
                     LAAHKFEFSAEIAYNTFLKLILMPLALLLVGMACHLNSEHLQMMVLAGALPPAFSGII
                     IASRFNVYTRTGTASLAVSVLGFVVTAPLWIYVSRLVS"
     gene            complement(5175..6869)
                     /gene="oxc"
                     /locus_tag="ACHKBL_003986"
     CDS             complement(5175..6869)
                     /gene="oxc"
                     /locus_tag="ACHKBL_003986"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008949 - oxalyl-CoA decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HFO5820785.1"
                     /translation="MSDQLQMTDGMHIIVEALKQNGIDTIYGVVGIPVTDMARHAQAE
                     GIRYIGFRHEQSAGYAAAASGFLTQKPGICLTVSAPGFLNGLTALANATVNGFPMIMI
                     SGSSDRAIVDLQQGDYEELDQMNAAKPYAKAAFRVNQPQDLGIALARAIRVSVSGRPG
                     GVYLDLPANVLAATMEKDEALTTIVKVENPSPALLPCPKSVASAISLLAKAERPLVIL
                     GKGAAYSQADEQLREFIESAQIPFLPMSMAKGILEDTHPLSAAAARSFALANADVVML
                     VGARLNWLLAHGKKGWAADTQFIQLDIEPQEIDSNRPIAVPVVGDIASSMQGMLAELK
                     QNTFTTPLVWRDILNIHKQQNAQKMHEKLSTDTQPLNYFNALSAVRDVLRENQDIYLV
                     NEGANTLDNARNIIDMYKPRRRLDCGTWGVMGIGMGYAIGASVTSGSPVVAIEGDSAF
                     GFSGMEIETICRYNLPVTIVIFNNGGIYRGDGVDLSGAGAPSPTDLLHHARYDKLMDA
                     FRGVGYNVTTTDELRHALTTGIQSRKPTIINVVIDPAAGTESGHITKLNPKQVAGN"
     gene            complement(6923..>8167)
                     /gene="frc"
                     /locus_tag="ACHKBL_003987"
     CDS             complement(6923..>8167)
                     /gene="frc"
                     /locus_tag="ACHKBL_003987"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033608 - formyl-CoA transferase activity
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HFO5820786.1"
                     /translation="TPLQGIKVLDFTGVQSGPSCTQMLAWFGADVIKIERPGVGDVTR
                     HQLRDIPDIDALYFTMLNSNKRSIELNTKTAEGKEVMEKLIREADILVENFHPGAIDH
                     MGFTWEHIQEINPRLIFGSIKGFDECSPYVNVKAYENVAQAAGGAASTTGFWDGPPLV
                     SAAALGDSNTGMHLLIGLLAALLHREKTGRGQRVTMSMQDAVLNLCRVKLRDQQRLDK
                     LGYLEEYPQYPNGTFGDAVPRGGNAGGGGQPGWILKCKGWETDPNAYIYFTIQEQNWE
                     NTCKAIGKPEWITDPAYSTAHARQPHIFDIFAEIEKYTVTIDKHEAVAYLTQFDIPCA
                     PVLSMKEISLDPSLRQSGSVVEVEQPLRGKYLTVGCPMKFSAFTPDIKAAPLLGEHTA
                     AVLQELGYSDDEIAAMKQNHAI"
BASE COUNT         2367 a         1918 c         1714 g         2168 t
ORIGIN      
        1 ccgcgtcaat ataacttttt attaaccaga aaagattcta tcgttcttaa tgaagtactc
       61 aatcgatttg ttgatgcttt aacaaatgaa gttcgctatg aagtatcaca aaattggctt
      121 gatacaggaa atctggcctt tctgaacaaa ccattagaac tcactgaaca tgaaaaacag
      181 tggattaagc agcatcccga tttaaaggtg ctagaaaatc cttactcgcc accctattct
      241 atgacagatg aaactggctc agttcggggc gtgatgggtg atattcttaa tattattacc
      301 ttgcaaacag gtttaaattt ttctccgatc accgtttcac acaatattca tgctgggaca
      361 cagcttaacc ccggtggatg ggatatattg cccgccgcta tttatagtga agatcgagaa
      421 aataatgttt catttgctga agccttcata acaacgcctt acgtttttgt catgcaaaaa
      481 gcgcctgaca gtgaacaaac attaaaaaaa ggaatgaaag ttgccattcc ctattattat
      541 gagcttcatt cgcaattaaa agagatgtat ccggaggtag agtggataaa agtcgataac
      601 gccagcgctg catttcacaa ggttaaagaa ggtgaacttg atgctctggt cgcgacacag
      661 ttaaattcgc gttacatgat cgaccattac tatcctaatg aactttatca ttttcttatt
      721 cccggcgttc agaatgcatc actttcgttc gcttttcctc gcggagaacc ggaacttaag
      781 gatattatta ataaagcact gaatgcaatt cccccaagcg aagttctgcg cctgaccgaa
      841 aaatggatta aaatgcccaa tgtgaccatt gacacatggg acctctatag cgagcaattt
      901 tatattgtta cgacattatc cgttttatta gttggcagta gccttttatg gggattctac
      961 ctgttacgct cagttcgtcg tcgtaaagtt attcagggtg atttagaaaa ccaaatatca
     1021 ttccggaaag cgctctcgga ttccttaccg aatccaactt atgttgtaaa ctggcaaggt
     1081 aatgtcatta gtcacaatag tgcatttgaa cattatttca ctgctgatta ctataaaaat
     1141 gcaatgttac cattagaaaa cagtgaatcc ccctttaaag atgttttttc taatacgcat
     1201 gaagtcacag cagaaacgaa agaaaaccga acaatataca cacaggtatt tgaaattgat
     1261 aatggcatcg agaaaagatg cattaatcac tggcatacct tatgtaatct gccagcaagc
     1321 gaacatgctg tttatatttg tggttggcaa gatattaccg agacgcgtga tttaattcat
     1381 gcactcgaag tagagagaaa taaagcgatc aatgcaactg tcgcaaaaag ccagtttctg
     1441 gcaacaatga gtcacgaaat aagaacacca ataagctcca ttatgggctt cctggaactt
     1501 ctgtcgggtt ctggtcttag caaggaacaa cgggtggagg cgatttcact tgcctacgcc
     1561 accggacaat cactcctcgg cttaattggt gaaatccttg atgtcgacaa aattgaatcg
     1621 ggtaactatc aacttcaacc acaatgggtc gatatcccta ctttagtcca gaacacttgt
     1681 cactctttcg gtgcgattgc tgcaagcaaa tcgatcgcat taagttgcag cagtacgctt
     1741 cctgaacgct acctggttaa gatcgaccct caggcgttta agcaggtctt atctaattta
     1801 ctgagtaatg ctctcaaatt tactaccgag ggggcagtaa aaattacgac ctccctgggt
     1861 cacattgatg acaaccacgc tgttatcaaa atgacgatta tggattctgg aagtggatta
     1921 tcgcaggaag aacaacaaca actgtttaaa cgctatagcc aaacaagtgc aggtcgtcag
     1981 caaacaggtt ctggtttagg cttaatgatc tgcaaagaat taattaaaaa catgcagggc
     2041 gatttgtcat tagaaagtca tccaggcata ggaacgatat ttacgatcac aatcccggta
     2101 gaaattaccc aacaagtggc ggctgtcgag gcaaaagcag aacaacctat aacactacct
     2161 gaaaagttga gcatattaat cgcggatgat catccgacca acaggctatt actcaaacgc
     2221 cagctaaatc tattaggata tgatgttgat gaagccactg atggtgtgca agcgctacac
     2281 aaagtcagta tgcaacatta tgatctgctt attactgacg ttaatatgcc gaatatggat
     2341 ggttttgagt tgactcgcaa actccgagag caaaattctt ccttacccat ctgggggctt
     2401 acagccaacg cacaggctaa cgaacgtgaa aaagggttaa attgcggcat gaacttatgt
     2461 ttgttcaaac cgttgactct ggatgtactg aaaacacatt taagtcagtt acaccaggtt
     2521 gcgcatattg cacctcagta tcgccacctt gatatcgagg ccctgaagaa taatacggcg
     2581 aatgatctac aactgatgca ggagattctc atgactttcc agcatgaaac gcataaagat
     2641 ctacccgctg cgtttcatgc actagaagct ggcgataaca gaactttcca tcagtgtatt
     2701 catcgcatcc acggtgcggc taacatcctg aatttgcaaa agttgattaa tattagccac
     2761 cagttagaaa taacacccgt ttcagatgac agtaagcctg aaattcttca gttgctgaac
     2821 tctgtaaaag aacacattgc agagctggac caggagatta ctgttttctg tcagcaaaat
     2881 gactaaatag cggctcccac aatgttcaaa tgtgggagct atttaccagc acatcttaca
     2941 tttatgatga gaactcctgg cgaatttgtt ccccatgctg gtcgagcgtt gccgctcccg
     3001 gcataacatg cgggtccgcg cagccgctga tttttatcgg attccccggc atcattattc
     3061 ccccggcttc aatcaacata tttctcgcct gagtttgtgg caaattaatg gcctcagcca
     3121 cacttaataa cggcgcgacg ggtacaccaa cttcatgtat tctggctaac caaacttcag
     3181 ctgcctgcgt ttttaacgtc cgctcaatat attgtttaag aatagcctgg ttttgtacgc
     3241 gtaaaatatt gctgctaaat ctgggatcat taaccagttc cgtaagctcc agtgcctggc
     3301 ataacgcaga aaaaagcttg tcattaccac aacaaatcgt aatcggctta tcctgagtat
     3361 cgaaaacatc aaaaggtgcc atgtagggat ggcgatttcc caggcgttgt ggtgacttcc
     3421 ctgtcgcaat atatgccatc agaccatgct ccagaaaact cagcgtggca tcaaacatcg
     3481 ctatatcgac atgcgcccct ctttggctct tttcgcggcc ataaagtgca ctcactattc
     3541 cgctgaataa atagacaccg ccgcatagat ccgcaagaga ggtacccacg cgcactggcg
     3601 gagcatcagg gtatcctgtt tccatcatta tcccgctcat tgcctgaatg atggtatcgt
     3661 aggcaggagc atcttttagc ggaccggtat gtccgaaacc tgacgatgaa gcatatataa
     3721 ggcgcgggtt gatttcttgt aacctttccc atgaaaaccc gagtttttcc attgtacctg
     3781 ggcgaaaatt ctcagctaat acatccgctt gtttaagcat atttataaat atacttttat
     3841 cgtgatcatt ctttaaatca agaaccacac tctctttgcc atgattaata aaactgtaat
     3901 agagtgactg tccatccaca tagggaccaa atgtgcgggt atcatcacca tgacccggcg
     3961 gctcaacttt aattaccctt gcgcccatat tacaaagaag ttgagttccg aaaggcccat
     4021 taaggacatg cgtcatatcg ataactaata agccttcaaa cggccctttg cattcattat
     4081 ttgtcatttg cttatgcctt gaaaaaagcc gaaggtgaaa agacacttcg gcatttatgt
     4141 aaaatcaata acatgattaa ttatgaaacc agtcgactga cataaatcca caaaggagcc
     4201 gtgacgacaa aacccagtac gctcacagcc aatgacgctg taccagtgcg ggtgtagaca
     4261 ttaaaccggc tggcaataat gattccggag aatgccggcg gtaaggcgcc tgccagtacc
     4321 atcatctgca aatgttcgct gttcaaatga catgccatac caacgagaag cagcgccagt
     4381 ggcatcagaa tcagcttcag gaaggtgtta taagcaattt cggcactgaa ctcgaattta
     4441 tgtgcagcca gagtcaaccc cgcagcgaat accgctaccc ctgagttagc cttcgcaatc
     4501 agattaaagg ttgggtccca tgctgccgga atttttaccc caaccaacac caggatcgtt
     4561 gccagaacag gtgcccatac tactggctcc tttgccgcag aaattaatgc gctcagatta
     4621 ctattcttct taccatccgc tcctgaggaa ggattcagca aatacagacc aataggaata
     4681 gtaattgcat taacaataat agaaataatt gctaccacta aacctgttga tacggaatca
     4741 ccataaatag gatcgagaac tgcaaatccc aggaatccaa tggtaggtga acctgcaatt
     4801 aatgcacata ctgctgcttc tgcatgagta cgtttaaaaa atttgtagca accgaaccag
     4861 gagaaaaaga aacatccgac aataaccaca agtgatacaa gggtcagacg agtgtccgca
     4921 aaaatcattt ccctgtttgc ccgggtaata gatacaaata gagctgcagg aagagcgtag
     4981 tttaatacca gtttattaaa tgcccgggct tgatcttctg aaaaagtttc tcgtctgccg
     5041 ctaaaataac ccaacagcat gattacgata ataggcaata aatcgccaat aaaaaatgtt
     5101 agcataatag tatttccgat aagggtcccc tcaccatcgc ataatgagtt ggcaagagga
     5161 gacgatgtca gtatttaatt accagcgact tgttttgggt taagtttggt aatatggcca
     5221 ctttcagttc ctgctgcagg gtcgatgacc acattaataa tggtcggttt gcgcgactga
     5281 ataccggtgg ttaaagcatg acgaagttca tctgtcgtag tgacgttata gccaacgcca
     5341 cgaaacgcat ccattaattt gtcatacctt gcatggtgca gcagatccgt tggtgatggt
     5401 gcaccagcgc cgctgagatc aacaccgtct cctctgtaga tgccgccatt attaaaaata
     5461 acgatcgtca ccggcaggtt atatcgacaa atcgtttcaa tttccatccc actgaaacca
     5521 aaagcactat caccttcaat ggcaacaacc ggagaaccag aggtcacgct agcaccgatg
     5581 gcatagccca taccgatgcc catgacaccc caggtgccac aatccagacg acgacgtggt
     5641 ttatacatat caataatatt tcgtgcatta tccagggtat ttgccccttc attaactaaa
     5701 taaatatcct ggttctcgcg caatacgtcg cgcacagcac ttaatgcatt aaagtaattt
     5761 aatggttgag tatctgtgct taacttttca tgcatttttt gtgcattttg ctgcttgtgg
     5821 atatttaaaa tatcgcgcca taccagtgga gtcgtaaatg tgttttgttt cagttctgcc
     5881 agcatacctt gcatactgga tgcaatatcg ccaacgactg gcacagcaat ggggcggttg
     5941 ctgtcaattt cctgcggttc aatatccagt tgaataaact gtgtatctgc cgcccatcct
     6001 tttttaccgt gtgccagtaa ccaattcagt cgtgcaccaa caagcatgac aacgtcagca
     6061 tttgccaggg caaacgaacg cgcagccgcc gcagaaagtg gatgcgtatc ttcgaggatc
     6121 cctttcgcca tagacattgg caggaatgga atctgggcac tttcaataaa ttcacgaagc
     6181 tgttcatccg cttgtgaata cgccgcgcct ttgccaagga taactaatgg tcgttcagct
     6241 tttgctaata gcgaaattgc gctagcgact gacttcgggc atggcaataa tgctggcgac
     6301 ggattttcaa cttttacaat cgtggttaac gcttcgtctt tttccatcgt cgcggccagg
     6361 acatttgctg gcaaatcaag ataaactccg ccagggcgac ccgatactga gacccggata
     6421 gcgcgtgcca atgcaatgcc aagatcctgc ggctgattaa cgcggaatgc tgctttggca
     6481 tacggttttg ccgcattcat ttggtccagc tcttcataat caccttgctg taggtcgacg
     6541 atcgcgcggt cgctggagcc gctaatcatg atcatcggaa aaccatttac cgttgcgttg
     6601 gccaatgcgg tcaaaccatt gaggaatcct ggcgcagaaa ctgtcaggca gatccccggt
     6661 ttttgggtaa gaaaaccgct tgccgcagcg gcatagcctg ccgactgctc atgacgaaaa
     6721 ccaatataac gaatgccttc cgcctgggca tggcgcgcca tatccgtcac aggaatacct
     6781 acaacaccat aaatagtgtc aataccattc tgttttaatg cttcaacgat gatatgcata
     6841 ccatctgtca tttgaagttg atctgacatg attaataacc tcgaaatttt tataaattgg
     6901 gagccagaag cccccgttaa tatcagatgg cgtggttttg cttcattgca gcaatttcat
     6961 cgtcgctata acccagctcc tgcaatacag cagcggtatg ctcacctaat agcggcgcag
     7021 ctttaatatc cggcgtaaag gcagagaatt tcattggaca accaacggtc agatattttc
     7081 cacgcaacgg ttgttccact tcgacaacac taccactttg gcgcaaagag ggatcaagtg
     7141 aaatttcttt catacttaaa accggtgcac aaggaatatc aaactgagtc aaataggcca
     7201 ccgcttcatg tttatcaata gtgacagtgt atttttcgat ttcagcaaaa atatcgaaaa
     7261 tatgtggctg tcgggcatgg gctgtactgt atgccggatc ggtaatccat tctggtttgc
     7321 cgatggcttt acaggtgttt tcccagtttt gctcctgaat agtgaaataa atataggcgt
     7381 taggatcggt ttcccagcct ttacatttca ggatccagcc aggctgaccg ccaccacccg
     7441 cattaccacc gcggggaact gcatcaccaa atgtgccatt cggatactgc gggtattctt
     7501 ccagataccc caacttatcg agacgctgct ggtcgcgtaa tttcacgcgg caaaggttca
     7561 acacggcatc ctgcattgac atggtgactc gttgcccacg ccccgttttt tcgcgatgca
     7621 gcaaagcagc aagtaaaccg atcagcaaat gcattccggt gttgctgtca cccaacgctg
     7681 cagcgcttac cagcggcgga ccgtcccaaa aacctgtagt agatgccgcg ccacccgctg
     7741 cctgagcaac gttttcatag gcttttacat tcacataagg cgaacactca tcaaaccctt
     7801 tgatcgaacc aaaaatcaga cgtggattga tttcttgaat atgctcccag gtgaagccca
     7861 tatgatcaat ggcccctgga tgaaagttct cgactaagat atcagcttcg cggatcagct
     7921 tttccattac ctctttgcct tccgctgttt tggtatttaa ttcaatagaa cgtttgttac
     7981 tgttaagcat ggtgaagtaa agcgcatcga tatcaggaat atctcgcagc tggtggcgcg
     8041 ttacgtcacc aacgccggga cgttcaattt taatgacgtc agcgccaaac caggccagca
     8101 tctgagtaca agatgggcca gattgcacac cagtgaaatc gagaacttta attccttgaa
     8161 gtggagt
//
LOCUS       DAZFLD010000198         7977 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.199, whole genome shotgun sequence.
ACCESSION   DAZFLD010000198 DAZFLD010000000
VERSION     DAZFLD010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7977
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.199"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..453
                     /locus_tag="ACHKBL_003988"
     CDS             <1..453
                     /locus_tag="ACHKBL_003988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HFO5820787.1"
                     /translation="RGYAKYLEADNLMDDASDMVLFDELVFTRARALSVTKALKGMFA
                     DLEEATDVETRVALYDKILKAEQALDRNIARIESIERSLLTLDVLAETAPKLRADRER
                     INAARDKLRAETDILTSQRRGVITPVSDIVSSLHEMSNSGRLDDIPEE"
     gene            434..2140
                     /locus_tag="ACHKBL_003989"
     CDS             434..2140
                     /locus_tag="ACHKBL_003989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HFO5820788.1"
                     /translation="MTFRKNKTRCDEPAEMTETEQRLFIMTKLSNPWWRLNHLYKIQN
                     EKGELVTFRMRPAQRQLFRNMHNKNIILKARQLGFSTAIDIYLLDQALFIPHLKCGIV
                     AQDKQAASEIFRTKIAVPFDHLPDWLRASFTIVERRSGASGGYILFGHGSSIQVATSF
                     RSGTVQRLHISEHGKICAKYPAKAKELRTGTLNAVSDECIIFDESTAEGVGGDFYEMS
                     NRAQESTASGLLLTAQDYKFHFYAWWQDPKYSARVPESGLKLSREKMTYFSAVEKAMN
                     ITLTDEQKQWYISKETEQREEMKQEFPSTPQEAFLTSGRRVFSAESTLQAESFCSPPM
                     IVYDIEPVTGAKTKAQSLREGNKNELQRTLMNYLLVWELPDPDEEYVCGADTAEGLEH
                     GDRSSLDVVKRSNGEQVAHWFGHLDAELFAHLISQVCRMYNNAFVGPERNNHGHAVIL
                     KLRELYPTRYIYNEQHLDQAYDDDTPRLGWLTTRQSKPVLTEGMKTLLNNGISGIRWS
                     GTLSEMNTYVYDAKGSMNAQEGCFDDQLMSYMIAQEMRARMPVRVKQKTDKRRTTHWM
                     AH"
     gene            2140..4284
                     /locus_tag="ACHKBL_003990"
     CDS             2140..4284
                     /locus_tag="ACHKBL_003990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HFO5820789.1"
                     /translation="MKNEINTTAMKNDHGSTPRFSQRQLLSLCSDIDSQPLWRDAANK
                     ACAYYDGDQLAPEVIQVLKDRGQPMTIHNLIAPTVDGVLGMEAKTRTDLIVMSDDPND
                     ETEKLAEAINAEFADACRLGNMNKARSDAYAEQIKAGLSWVEVRRNSDPFGPEFRVST
                     VSRNEVFWDWLSRESDLSDCRWLMRRRWMDTDEAKATFPGMAQVIDYAIDDWRGFVDT
                     TITEGQPSPLMSAWEEYQSWDRRENEWLQRERRRVLLQVVYYRTFERLPVIELSNGRV
                     VAFDKNNLMQAVAVASGRVQVKVGRVSRIREAWFVGPHFIVDRPCSAPQGMFPLVPFW
                     GYRKDKTGEPYGLISRAIPAQDEVNFRRIKLTWLLQAKRVIMDEDATQLSDSDLMEQI
                     ERPDGIIKLNPARKNQKSVADVFRVEQDFQVASQQFQVMQESEKLIQDTMGVYSAFLG
                     QDSGATSGVAISNLVEQGATTLAEINDNYQFACQQVGRLLLAYLLDDLKKRRNHAVVI
                     NRDDRQRRQTIVLNAEGDNGELTNDISRLNTHIALAPVQQTPAFKAQLAQRMSEVIQG
                     LPPQVQAVVLDLWVNLLDVPQKQEFVERIRAALGTPKSPDEMTPEEQEVAAQQQALQQ
                     QQAELQMREMAGRVAKLEADAARAHAAAQRDNASAQREVALTQGQRYVDALNQAHTAE
                     IITGVQNMEQEQDVLQQQMLYTLQQRMNEMSL"
     gene            4442..5449
                     /locus_tag="ACHKBL_003991"
     CDS             4442..5449
                     /locus_tag="ACHKBL_003991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5820790.1"
                     /translation="MDFEFTGEETPEQLEKMLEGLGDVDIDSHAQDVVTEDTTEKHAD
                     EEAQTQTGDNNVAPTPDASVEQTQDVKEPEAKGVLTRDGKHVIPYEVLEAERSGKQRA
                     EQEAALLRGQIAEEKRRVELLTSQIHQAGMKPTPLPENEKISDEQIARIREMYPEIGD
                     AVASLIRKNNYLQSRVQQSTQQAEGNGGEDLSPVLDAMNAVPVLKTWQESDPDRFSVA
                     VSIDGKLQNDPAWKDKTLTERFAEVARRTQVAFGEVSESSADNQADKTDIRKTAEEKV
                     KTAEQEQAVPASPSDLGTTASVGTGDNFERLLGASHSEAEAIMRGMTNAEIDALLEKL
                     G"
     gene            5473..6687
                     /locus_tag="ACHKBL_003992"
     CDS             5473..6687
                     /locus_tag="ACHKBL_003992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019028 - viral capsid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N4-gp56 family major capsid protein"
                     /protein_id="HFO5820791.1"
                     /translation="MTTVTSAQANKLYQVALFTAANRNRSMVNILTEQQEAPKAVSPD
                     KKSTKQTSAGAPVVRITDLNKQAGDEVTFSIMHKLSKRPTMGDERVEGRGEDLSHADF
                     SLKINQGRHLVDAGGRMSQQRTKFNLASSARTLLGTYFNDLQDQCAIVHLAGARGDFV
                     ADDTILPTAEHPEFKKIMINDVLPPTHDRHFFGGDATSFEQIEAADIFSIGLVDNLSL
                     FIDEMAHPLQPVRLSGDELHGEDPYYVLYVTPRQWNDWYTSTSGKDWNQMMVRAVNRA
                     KGFNHPLFKGECAMWRNILVRKYAGMPIRFYQGSKVLVSENNLTATTKEVAAATNIDR
                     AMLLGAQALANAYGQKAGGHFNMVEKKTDMDNRTEIAISWINGLKKIRFPEKSGKMQD
                     HGVIAVDTAVKL"
     gene            6743..7132
                     /locus_tag="ACHKBL_003993"
     CDS             6743..7132
                     /locus_tag="ACHKBL_003993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5820792.1"
                     /translation="MAKTILAPSLSERVYTGTHGNESVAEGVFTVNAAEADSVIHLLS
                     LPVGIRINSLQLVSTGGLGTATVSIKSGEHALIDNSEAVSAKFARYVPVEPYTTQRDG
                     ELVTVTIKTAAATGTLNVLLRYTVVGY"
     gene            7182..7643
                     /locus_tag="ACHKBL_003994"
     CDS             7182..7643
                     /locus_tag="ACHKBL_003994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5820793.1"
                     /translation="MSEKIAVVYIGPKPVKKDTITGSRTLFPRLEPVHVDSAMAWQLL
                     GFPDVWVRHEELDDVLKKQQQNEQLRQAQQAQESVLAARAEAENSFVVSVNGQEVDLS
                     KLTSARLATLCEAEELDIHKDPKETAEAFRIRVREAFRRRVAETEQHGGTE"
     gene            7627..>7977
                     /locus_tag="ACHKBL_003995"
     CDS             7627..>7977
                     /locus_tag="ACHKBL_003995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5820794.1"
                     /translation="MAELSDFLPYVRRHISGPLNIMMTDALSMAAVAFSRQSLVCRRE
                     VTVVPVAGKEIVLPYDKDDEECVHIIRISDDNHELFVGRDVDISSGRSLRFACSPGEV
                     SVLYAVAPKAGRSQI"
BASE COUNT         2048 a         1866 c         2256 g         1807 t
ORIGIN      
        1 cgtggatatg cgaagtatct tgaggcagat aacctcatgg atgatgcgtc cgacatggtg
       61 ctgttcgatg aactggtgtt cacccgggcc cgcgcacttt cagtaactaa ggcacttaaa
      121 gggatgttcg ccgacctgga agaggcaact gacgtggaaa cccgcgttgc tctttacgac
      181 aaaatactca aagctgaaca ggcccttgac cggaatattg cccgtatcga gtcaattgaa
      241 cgctcattgc tgacgctgga cgtcctggct gagacagcac caaaacttcg tgctgaccgg
      301 gaaagaatca acgccgccag agataaactc agagcggaaa ccgatattct gaccagccag
      361 cgccgggggg ttattacgcc agtcagtgac atcgtgtcat cgctgcatga aatgagtaat
      421 tcggggagac tggatgacat tccggaagaa taaaacgcga tgtgatgaac ctgcagaaat
      481 gactgagacc gaacaacgtc tgttcattat gacaaaactg agcaatccct ggtggcgcct
      541 caatcatctc tacaaaatac agaacgaaaa aggtgaactg gtcaccttca gaatgcgacc
      601 ggcgcaacgt cagttgtttc gtaacatgca taataaaaac attatcctga aagcgcgcca
      661 gctgggattt tccacagcca ttgatattta tcttctcgac caggcattat tcattccgca
      721 tctcaaatgc gggatcgtcg ctcaggataa acaggctgcc agtgaaattt tccgcacaaa
      781 aattgctgta ccgtttgatc atctccctga ctggctgaga gcctcattca ccatcgttga
      841 acgtcgtagc ggtgccagcg gtggctatat cctgtttggt cacggctcga gtattcaggt
      901 ggcaacctca tttcgctcag gtacggtgca gcgcctgcat atctcagagc acggcaaaat
      961 ttgcgcgaaa tatccggcta aggcgaaaga gctgcgaacc ggtacgctta atgccgtctc
     1021 tgatgaatgc attatttttg atgaatccac ggctgaaggc gtgggtggtg atttttacga
     1081 gatgagtaac cgtgcacagg agagcactgc atcaggctta ttgctgacgg cacaggatta
     1141 taaattccat ttttacgcat ggtggcagga tcctaaatac agcgccagag tgcctgaaag
     1201 cgggctgaag ctgtcacggg aaaaaatgac gtatttttct gcggttgaga aggcaatgaa
     1261 catcacgctt accgatgagc agaagcagtg gtacatcagt aaggaaactg aacagcgtga
     1321 ggaaatgaag caggagtttc cctcaacgcc acaggaggcg tttctgacgt ccggacgacg
     1381 tgtgttcagt gccgaaagta cattgcaggc agaatcattc tgttcgccac cgatgattgt
     1441 ttatgacatt gaacctgtta caggagcgaa gactaaagct cagtctctgc gtgaaggaaa
     1501 taaaaacgag ttgcagcgga cgctgatgaa ttatctgctg gtatgggaac tgccggatcc
     1561 ggatgaagag tatgtttgtg gggcagatac tgccgaaggg ctggagcacg gagaccgctc
     1621 atcgctggat gttgtcaaac gcagtaatgg cgagcaggtg gctcactggt tcgggcatct
     1681 cgatgctgaa ctttttgctc atctcatttc gcaggtctgt cgtatgtata acaacgcgtt
     1741 tgtggggccg gagcgtaata atcacggaca tgcagttatc ctgaaactcc gggaactcta
     1801 tccgacacgt tatatctaca acgaacagca tcttgaccag gcatatgacg acgatacgcc
     1861 ccgccttggc tggctgacaa cccgtcagag caaacctgtt ctgaccgaag gaatgaaaac
     1921 gctcctgaat aatggaatat cagggatccg ctggtcaggc acattatcgg aaatgaacac
     1981 ctacgtttat gacgcgaaag gctccatgaa tgcacaggaa ggctgctttg atgatcagct
     2041 catgagctac atgattgccc aggagatgcg cgccagaatg ccggtgaggg taaaacagaa
     2101 aacggataaa cgcagaacca cacactggat ggcacactga tgaaaaatga aattaacacc
     2161 acagcgatga aaaacgatca tggatccacg ccgcgttttt ctcagcgtca gttactgtct
     2221 ctctgttctg atattgacag tcagcctctc tggcgtgatg ccgcaaacaa ggcctgtgcg
     2281 tattatgatg gcgaccagct ggcaccggaa gttatccagg tactgaaaga tcgcggtcag
     2341 cccatgacca tccataacct cattgccccc acggtagatg gtgtactggg aatggaggca
     2401 aaaacaagaa cggacctgat agtgatgtca gacgatccga acgatgaaac agagaaactg
     2461 gcagaggcca ttaatgcgga gtttgctgat gcgtgccgtc ttggcaacat gaataaggcc
     2521 cgctccgatg cctatgcgga acaaatcaag gcggggctca gttgggtgga ggtcaggcgc
     2581 aacagcgatc cgttcggacc tgaattcagg gtatccacag tcagcagaaa tgaggttttc
     2641 tgggactggc tcagccggga atccgacctg agtgactgcc ggtggctgat gcgtcgccgc
     2701 tggatggata ccgatgaggc aaaagccacg ttcccgggaa tggctcaggt tatcgattat
     2761 gctattgatg actggcgtgg ttttgtcgat accacgatta ctgaaggcca gcccagtccg
     2821 ttgatgagtg catgggaaga gtatcagtca tgggatcgca gggagaatga atggcttcag
     2881 cgtgaacgcc gtcgtgtgct gcttcaggtg gtttattacc gtacattcga acgtcttccg
     2941 gtgattgaac tcagtaatgg acgggtggtg gcttttgata aaaataatct gatgcaggcg
     3001 gtggctgtgg catccgggcg ggttcaggtg aaagtcgggc gggtaagtcg tattcgtgaa
     3061 gcctggtttg tgggcccaca ctttattgtg gatcgcccct gtagtgctcc gcaggggatg
     3121 tttccgctgg ttcctttctg gggataccga aaggataaaa ccggggagcc atacgggtta
     3181 atttcccgcg ccattccggc acaggatgag gtgaattttc gtcgtatcaa gctgacctgg
     3241 ctgcttcagg ccaaacgcgt gattatggac gaggatgcca cccagttgtc agacagcgac
     3301 ctgatggagc agatcgaacg tccggatggc attattaaac tgaatccggc ccgaaaaaat
     3361 cagaaaagtg tcgcagatgt ttttcgggtt gagcaggatt ttcaggttgc cagccagcag
     3421 tttcaggtca tgcaggaatc ggaaaaactt atccaggata ccatgggagt gtattccgca
     3481 tttctcgggc aggattcagg tgcgacgtca ggcgtggcta tcagtaacct ggtggagcag
     3541 ggggccacaa ctcttgcgga aatcaacgat aactaccagt ttgcctgcca gcaggtggga
     3601 agactgttgc tggcttatct tctcgatgac ctgaaaaaac gccgtaatca tgcagtggtg
     3661 attaatcgcg atgatcgcca gcgtcgccag accattgtcc tcaatgctga aggtgataat
     3721 ggtgaactga ccaatgatat ttcaaggtta aatacacata ttgcgctggc gcctgttcag
     3781 cagacaccgg cgtttaaggc acagcttgca cagagaatgt cagaggttat tcaggggctg
     3841 ccgcctcagg tgcaggctgt tgtgctcgac ctgtgggtta atcttctgga tgtgccgcag
     3901 aaacaggagt ttgttgagcg tattcgtgct gcgctgggga cgccaaaatc accggatgaa
     3961 atgacgccgg aagaacagga agtagcggca caacaacagg cacttcagca acaacaggca
     4021 gaactccaga tgcgcgagat ggctggcaga gtggcaaaac tggaagctga cgccgccagg
     4081 gcacatgcag ctgcacaacg ggataatgcc agtgcacagc gggaagtcgc cctgacacag
     4141 gggcagcgtt atgtggatgc gcttaaccag gcacatacgg cagaaatcat taccggcgta
     4201 cagaatatgg aacaggagca ggacgttctt cagcaacaga tgctgtatac gttacaacag
     4261 cggatgaatg aaatgtcgct ctgaaaactc tggcttcaac tgaaccccgt catcgtacgg
     4321 ggttttttgt ttccggaggt aagcgttccg ggagcggtgc gcttattcgc gggggcagcg
     4381 ataagcctta tttactcaac cattcggatc tgtccgataa acagaccatg cggagttatt
     4441 tatggatttt gaatttacgg gtgaagaaac cccggaacaa ctggaaaaaa tgctggaagg
     4501 acttggggat gtggatattg acagtcacgc acaggacgtc gtgacggaag ataccacgga
     4561 aaaacatgcg gatgaggaag cacagactca gacgggcgat aacaatgtgg caccgacgcc
     4621 ggatgccagt gtggagcaga cgcaggacgt gaaggagccg gaagcgaagg gggtgctcac
     4681 ccgcgacggt aaacacgtca ttccctatga agtccttgag gctgaacgtt ccggtaagca
     4741 acgggccgaa caggaagccg cacttcttcg tgggcagata gctgaagaaa aacgcagggt
     4801 ggaactgctg acgtctcaga tccaccaggc cggtatgaag cccacaccgt taccggaaaa
     4861 cgaaaaaatt tctgatgagc agattgcccg tatcagggag atgtatccgg aaattggtga
     4921 cgcggtggct tcgctcatcc gtaaaaataa ctatctccag tcccgtgttc agcaatcaac
     4981 acagcaggca gaaggtaatg gtggtgagga tttatcaccg gttcttgatg cgatgaatgc
     5041 cgtgccggtg ctgaaaacgt ggcaggagtc cgatccggat cgcttctcgg ttgctgtatc
     5101 catcgacggg aagctccaga atgaccccgc atggaaagac aaaacgctca ctgaacgttt
     5161 cgctgaagtg gcccgtcgta cgcaggttgc tttcggtgaa gtcagtgagt cgtctgctga
     5221 caaccaggca gacaaaacgg atatccggaa aacggcggaa gaaaaagtga agaccgctga
     5281 acaggagcag gcagtacctg cttccccgtc agatttaggc accacggcct ccgtcggaac
     5341 cggtgataat tttgaacggt tacttggtgc ttctcattca gaggctgagg cgattatgcg
     5401 tggtatgacg aatgctgaaa tagacgcact tctggagaag ctcgggtaac ttactgaagg
     5461 agtactgaag taatgacgac tgtaacatca gcccaggcga ataagctgta tcaggtggcg
     5521 ctttttactg ctgccaaccg caaccgctcg atggttaata tccttactga acagcaggaa
     5581 gcgccaaaag cggtttcgcc ggacaagaaa agcacgaagc agaccagcgc aggtgcgccg
     5641 gttgtccgta tcacagacct taacaaacag gccggtgatg aagtgacctt cagcatcatg
     5701 cacaaactct caaaacgtcc gacgatggga gatgagcgtg ttgaaggtcg tggtgaggat
     5761 ctcagccatg ctgacttctc cctgaaaatc aatcagggac gtcacctggt ggatgcaggc
     5821 ggacgtatga gtcagcagcg cacgaagttt aacctggcat cctctgccag aacgcttctg
     5881 gggacgtact ttaatgacct gcaggaccag tgtgcgatag tgcatcttgc cggagcacgt
     5941 ggtgattttg ttgctgacga cactattctg ccgacagcgg agcaccctga attcaaaaaa
     6001 atcatgatca acgatgtact gcctccgaca catgaccgtc acttttttgg cggtgatgcg
     6061 acaagctttg agcagattga agcggcagat attttttcta ttggcctggt ggacaatctc
     6121 tccctgttca ttgacgaaat ggcgcatccg ttacagccgg ttcgtctgtc cggtgatgaa
     6181 cttcacggag aagatccata ttacgtcctg tacgtcacgc cgcgtcagtg gaatgactgg
     6241 tacacctcga cgtccggtaa ggactggaac cagatgatgg ttcgtgccgt gaaccgtgca
     6301 aaaggtttta atcatccgct gttcaaaggt gaatgtgcga tgtggcgcaa tatcctggtt
     6361 cgtaagtatg cgggtatgcc gatccgtttc tatcaggggt caaaggttct ggtatcagag
     6421 aataacctga cggcaaccac gaaagaggtc gctgctgcaa ccaatattga ccgcgccatg
     6481 ttactggggg ctcaggcgct ggcaaatgct tacggtcaga aggcgggcgg tcacttcaac
     6541 atggttgaga agaaaacgga tatggataac cgtactgaga tagcaatcag ctggatcaac
     6601 ggtctgaaaa aaatccgttt ccccgagaag agcggcaaga tgcaggatca cggcgtgatt
     6661 gccgttgata cagcagtgaa gctctgattt tttcctttcc ccatgccggg ttttcgcccg
     6721 gctttttcag gagtcattaa ttatggcaaa gactatcctt gccccgtcac tgagtgaacg
     6781 ggtctatacg ggtacgcacg gtaatgagtc ggtggcagaa ggcgtattta cggtgaatgc
     6841 tgcggaagcg gacagtgtta ttcatcttct ctcactgcca gtgggcatcc gtatcaactc
     6901 actccagctg gtttcaacgg gtggtctggg tactgcaacc gtcagcatta agtccggtga
     6961 gcatgctctc atcgataaca gcgaagctgt ttctgcaaaa tttgccagat atgtgccagt
     7021 ggagccgtac accacacagc gtgacgggga gctggttact gtcaccatta agactgccgc
     7081 tgcaaccggc accctgaatg ttctgctgcg ttataccgtg gtgggatact gattaaaacc
     7141 ttccggcccg cgtcatgcgg gctttttatc cggggaatta tatgagtgag aaaattgccg
     7201 ttgtctatat cggcccaaaa cccgtgaaaa aggacaccat taccggaagc cgcacattgt
     7261 tcccacgtct tgagccggtg catgttgaca gtgcgatggc ctggcaactg ctggggtttc
     7321 cggatgtctg ggttcgtcat gaagagcttg atgatgttct gaaaaagcaa caacagaatg
     7381 agcagttgcg gcaggcacag caggcgcagg aaagcgtgct tgctgcgcgg gcagaagcgg
     7441 agaacagttt tgttgtttct gttaacgggc aggaggtgga tttaagtaag ctcacctcag
     7501 cacggctggc gacgctgtgt gaggcagaag agctggatat tcacaaagac ccgaaagaaa
     7561 cggctgaggc attccgtatc cgggtgcgtg aggcatttcg ccgtcgtgtt gcggagactg
     7621 aacagcatgg cggaactgag tgatttttta ccgtatgtcc gtcgtcatat cagcggtcca
     7681 ctgaacatta tgatgacgga tgctctgtca atggctgccg tggcattcag ccgccagtcg
     7741 ttggtgtgcc gtcgggaggt tactgttgta ccggtggcag gaaaagaaat cgtgcttccg
     7801 tatgacaaag atgatgagga gtgcgttcat atcatccgta tctctgacga taatcatgag
     7861 ctttttgtcg gtcgggatgt ggatatcagc tccggacgct ccctgcgatt tgcctgttct
     7921 cccggtgagg tgagcgtgct ttatgccgtc gctccgaaag ccggacgcag ccagata
//
LOCUS       DAZFLD010000199         7967 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.200, whole genome shotgun sequence.
ACCESSION   DAZFLD010000199 DAZFLD010000000
VERSION     DAZFLD010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7967
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.200"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            112..1095
                     /locus_tag="ACHKBL_003996"
     CDS             112..1095
                     /locus_tag="ACHKBL_003996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001327875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HFO5820795.1"
                     /translation="MPEKYSPDDQSSTIDPYLITSVRQTLEEQGAALQNLSKQLDSGQ
                     YQCVLNLIMNCKGHVILSGMGKSGHVGRKMSATLASTGTPSFFIHPAEAFHGDLGMIT
                     PCDLLILISASGETDEILKLVPSLKNFGNRIIAITNNGNSTLAKNADAVLELHMANET
                     CPNNLAPTTSTTLTMAIGDALAIAMIHQRKFMPNDFARYHPGGSLGRRLLTRVADVMQ
                     HDVPAVQLDASFKTVIQRITSGCQGMVMVEDAEGGLAGIITDGDLRRFMEKEDSLTSA
                     TAAQMMTREPLTLPEDTMIIEAEEKMQKHRVSTLLVTNKANKVTGLVRIFD"
     gene            1167..2315
                     /locus_tag="ACHKBL_003997"
     CDS             1167..2315
                     /locus_tag="ACHKBL_003997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000905920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis protein"
                     /protein_id="HFO5820796.1"
                     /translation="MLIKVKSAVSWMRAHLSAISLSDIQKHLVKIIILAPMAVLLIYL
                     AIFSQPRYMSESKVAIKRSDDLNSGSLNFGLLLGASNPSSAEDALYLKEYINSPDMLA
                     ALDKQLNFREAFSHSGLDFLNHLSKDETAEGFLKYYKDRINVSYDDKTGLLNIQTQGF
                     SPEFALKFNQTVLKESERFINEMSHRIARDQLAFAETEMEKARQRLDASKAELLSYQD
                     NNNVLDPQAQAQAASTLVNTLMGQKIQMEADLRNLLTYLREDAPQVVSARNAIQSLQA
                     QIDEEKSKITAPQGDKLNRMAVDFEEIKSKVEFNTELYKLTLTSIEKTRVEAARKLKV
                     LSVISSPQLPQESSFPNIPYLIACWLLVCCLLFGTLKLLLAVIEDHRD"
     gene            2339..4015
                     /locus_tag="ACHKBL_003998"
     CDS             2339..4015
                     /locus_tag="ACHKBL_003998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016159830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HFO5820797.1"
                     /translation="MKLFKSILLIAACHAAQASAAIDISADPNLTGAAPLTGILNGQK
                     SDTQNMSGFDNTPPPAPPVVMSRMFGAQLFNGTSADSGATVGFNPDYILNPGDSIQVR
                     LWGAFTFDGALQVDPKGNIFLPNVGPVKVAGVSNSQLNALVTSKVKEVYQSNVNVYAS
                     LLQAQPVKVYVTGFVRNPGLYGGVTSDSLLNYLIKAGGVDPERGSYVDIVVKRGNRVR
                     SNVNLYDFLLNGKLGLSQFADGDTIIVGPRQHTFSVQGDVFNSYDFEFRESSIPVTEA
                     LSWARPKPGATHITIMRKQGLQKRSEYYPISSAPGRMLQNGDTLIVSTDRYAGTIQVR
                     VEGAHSGEHAMVLPYGSTMRAVLEKVRPNSMSQMNAVQLYRPSVAQRQKEMLNLSLQK
                     LEEASLSAQSSTKEEASLRMQEAQLISRFVAKARTVVPKGEVILNESNIDSVLLEDGD
                     VINIPEKTSLVMVHGEVLFPNAVSWQKGMTTEDYIEKCGGLTQKSGNARIIVIRQNGA
                     AVNAEDVDSLKPGDEIMVLPKYESKNIEVTRGISTILYQLAVGAKVILSL"
     gene            4025..4765
                     /gene="kdsB"
                     /locus_tag="ACHKBL_003999"
     CDS             4025..4765
                     /gene="kdsB"
                     /locus_tag="ACHKBL_003999"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001578967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HFO5820798.1"
                     /translation="MSKAVIVIPARYGSSRLPGKPLLDIVGKPMIQHVYERALQVAGV
                     AEVWVATDDQRVEKAVQAFGGKAIMTRNDHESGTDRLVEVMHKVEADIYINLQGDEPM
                     IRPRDVETLLQGMRDDPALPVATLCHAISAEEAAEPSTVKVVVNTHQDALYFSRSPIP
                     YPRNAEKARYLKHVGIYAYRRDVLQNYSQLPESMPEQAESLEQLRLMSAGINIRTFEV
                     AATGPGVDTPACLEKVRALMAQELAENA"
     gene            4762..6789
                     /locus_tag="ACHKBL_004000"
     CDS             4762..6789
                     /locus_tag="ACHKBL_004000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein"
                     /protein_id="HFO5820799.1"
                     /translation="MIGIFSSGIWRIPHLEKFLAQPCQKLSLLRPVPQEVDAIAVWGH
                     RPSAAKPVAIAKAAGKPVIRLEDGFVRSLDLSVNGAPPLSLVVDDCGIYYDASKPSAL
                     EKLVQDKAGNTALISKAREAMHTIVTGDLSKYNLAPAFVADESECSDIVLVVDQTFND
                     MSVTYGNAGQNEFSAMLEAAMAENPQATIWVKVHPDVLKGKKTGYFADLRATQRVRLI
                     TENISPQSLLRHISRVYVVTSQYGFEALLAGKPVTCFGQPWYAGWGLTDDRHPQSALL
                     SARRGSVTLEELFAAAYLRYCRYIDPLTGAASDLFTVLQWLQLLRNHQQQRNGYLWAP
                     GLTLWKSAILKPFLRTATNRLSYSRRCTAATACVVWGVKGEQQWQVEAQRKSLPLWRM
                     EDGFLRSSGLGSDLLPSLSLVLDKRGIYYDATRPSDLEVLLNHSQLTLAQQMRAEKLR
                     QRLVESKLSKYNLGADFSLPAEAKGKKVILVPGQVEDDSSIKTGTISIKSNLELLRTV
                     RERNPHAYIIYKPHPDVLVGNRKGDIPAELIAELADYQALDADIIQCIQHADEVHTMT
                     SLSGFEALLHGKHVHCYGLPFYAGWGLTFDEHHCGRRERELTLADLLYQALIVYPTYI
                     HPTRLQPITVEEAAEYLIQTSRKPMFITRKKAGWVIRYYRKLIMFCKVRFG"
     gene            6824..>7967
                     /locus_tag="ACHKBL_004001"
     CDS             6824..>7967
                     /locus_tag="ACHKBL_004001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001161851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis protein"
                     /protein_id="HFO5820800.1"
                     /translation="MQGNALTVLLSGKKYLLLQGPMGPFFSDVAEWLESLGRNAVNVV
                     FNGGDCFYCRHRHYQAYYQTPKEFPGWLRDLHRQYDFDTILCFGDCRPLHKEAKYWAK
                     SKGIRFLAFEEGYLRPQFITVEEGGVNAYSSLPRDPDFYRKLPDMPAPHVENLKPSTM
                     KRIGHAMWYYLMGWHYRHEFPRYRHHKSFSPWYEARCWVRSYWRKQLYKVTQRKVLPR
                     LMNELDQLYYLAVLQVYNDSQIRNHSNYNDVRDYINEVMYSFSRKAPKESYLVIKHHP
                     MDRGHRLYRPLIKRLSKEYGLGGRVIYVHDLPMPGLLRHAKAVVTINSTVGISALIHN
                     KPLKVMGNALYDIKGLTYQGHLHQFWQADFKPDMKLFKKFRGYILMK"
BASE COUNT         2050 a         1887 c         2065 g         1965 t
ORIGIN      
        1 ttatggaaaa gtagtatttt gatttatgtg aagatgagct gaactcgatt tatcagaggc
       61 aagttgtctt tatgttataa gtgttttaac cttatcggaa gtataattta tatgccagaa
      121 aaatattcac ctgatgatca gagcagtact attgatccat atttaattac ttctgttcgt
      181 cagaccttgg aagaacaagg tgcagcatta caaaacttgt ctaaacaact ggattccggg
      241 cagtaccagt gtgtccttaa tttgataatg aactgtaaag ggcacgttat tctttcggga
      301 atgggtaaat cagggcatgt cggtcgtaaa atgtcagcga cgctggcctc taccggtacc
      361 ccgagtttct ttattcatcc tgcagaagcg ttccatggcg atctgggtat gattacgccc
      421 tgcgatcttt tgatccttat ttctgccagc ggtgaaacgg atgaaatcct caagctagtt
      481 ccttcactga aaaatttcgg caaccgaatt atcgccatta ccaataatgg aaattccacg
      541 ctggcgaaaa atgctgatgc cgtgctggaa ctccatatgg cgaatgaaac ctgcccgaat
      601 aatcttgcac caacaacgtc taccaccctg accatggcga tcggcgatgc gctggcgatt
      661 gccatgatcc accaacgcaa atttatgccg aatgattttg cgcgctatca cccgggcggt
      721 tcattaggtc gtcgcctgct gacccgcgtt gctgatgtca tgcagcatga tgttcctgcg
      781 gtacagctgg atgcgtcatt taaaaccgtg attcaacgta tcaccagcgg atgccaggga
      841 atggtgatgg tagaagacgc agaaggtggg ctggcgggca ttataaccga cggtgacctg
      901 cgtcgcttta tggaaaaaga ggattctctg acatccgcta cggctgcgca gatgatgaca
      961 cgtgaaccgc tgacgctacc ggaagacacc atgatcattg aagcggaaga aaaaatgcaa
     1021 aagcaccgcg tctcaacgtt attggtgacc aacaaggcaa ataaagtcac tggccttgtg
     1081 cgcattttcg actaattaag caacggggtt tgagaggtta gtctctcatt gagtttgtgc
     1141 cccactggaa ctttgataat cgttacatgt tgataaaagt gaagtctgcc gtatcctgga
     1201 tgcgtgctca tctgtctgcc atctcactgt cagatatcca aaaacacctg gtgaaaatca
     1261 tcattctggc accgatggcg gtgctgctga tctatctggc tatcttcagc cagcctcgct
     1321 atatgagcga gtcgaaagtc gccattaaac gctcggatga tttaaacagc ggcagcctga
     1381 attttggtct gcttctgggt gcctctaacc ccagttccgc agaagatgcg ttgtatctga
     1441 aagagtacat caactcgccg gatatgctgg cggcgctgga taaacaacta aattttcgtg
     1501 aagcgttcag ccacagcggg ctcgattttc ttaatcatct tagcaaggat gaaaccgcag
     1561 aaggcttcct gaagtactac aaggaccgta tcaacgtctc gtatgacgat aaaaccggat
     1621 tactgaatat tcagacgcag ggctttagcc cggagtttgc gcttaagttt aaccagaccg
     1681 tgctgaaaga gtcagagcgc tttatcaatg agatgtcaca tcgcatcgcg cgtgaccagc
     1741 ttgcctttgc agaaacggag atggaaaagg cacgccagcg tctggacgcc agcaaagcgg
     1801 aattgctctc ttatcaggac aacaacaacg ttctggatcc acaggcacag gcacaggcgg
     1861 cgagcacgtt agtcaatacg ctgatgggcc agaagatcca gatggaagcg gatctgcgta
     1921 acttgctgac gtatctgcgt gaggacgccc cgcaagtggt gagtgcgcgt aatgcgattc
     1981 agtcattaca ggcacaaatc gatgaagaaa aaagcaaaat cactgcacca cagggtgaca
     2041 agctaaaccg tatggcggtg gattttgaag aaatcaaatc aaaagttgag ttcaacaccg
     2101 agctctacaa actgaccctg acctccattg aaaagacccg tgtagaagca gctcgtaagc
     2161 tcaaggtgct gtcagtgatc agttcgccac agttgccgca ggaatcgtct tttccaaata
     2221 tcccttattt gatcgcctgc tggttactgg tgtgctgcct gctgttcggc accctgaaac
     2281 tgttgctggc tgtcattgaa gatcaccgag actaacgctg tcgctgaatg agtttgtgat
     2341 gaaattattt aaatcaattt tactgattgc cgcctgtcac gcggcgcagg ccagcgcggc
     2401 cattgatatt agcgctgacc caaacctgac aggagccgcg ccgcttaccg gtattctgaa
     2461 cggacagaag tcggatacgc aaaacatgag cggcttcgac aataccccac cgcccgcacc
     2521 gccggtggta atgagccgta tgtttggtgc tcaacttttc aacggcacca gcgcggatag
     2581 cggtgcgacg gtaggattca accctgacta tattctgaat ccgggtgata gcattcaggt
     2641 tcgcttgtgg ggtgcgttca cctttgatgg tgcgttacag gttgatccca aaggtaatat
     2701 tttcctgccg aacgttggtc cggtgaaagt tgctggcgtc agtaatagtc agctaaatgc
     2761 cctggtcaca tccaaagtga aggaagtata ccagtccaac gtcaacgtct acgcctcctt
     2821 attacaggcg cagccagtaa aagtgtacgt gaccggattt gtgcgtaatc ctggtctgta
     2881 tggcggtgtg acgtctgatt cgttactcaa ttatctgatc aaggctggcg gcgttgatcc
     2941 agagcgcgga agttacgttg atattgtggt caagcgcggt aaccgcgtgc gctccaacgt
     3001 caacctgtac gacttcctgc tgaacggcaa actggggctt tcgcagttcg ccgatggtga
     3061 caccatcatc gtcgggccgc gtcagcatac tttcagcgtt cagggcgatg tctttaacag
     3121 ctacgacttt gagttccgcg aaagcagcat tcccgtaacg gaagctttaa gctgggcgcg
     3181 ccctaagcct ggcgcgactc acattacgat tatgcgtaaa caggggctgc aaaaacgcag
     3241 cgaatactat ccgatcagtt ctgcgccagg tcgtatgttg caaaatggcg ataccttaat
     3301 cgtgagcact gaccgctatg ccggtaccat tcaggttcgg gttgaaggcg cgcactccgg
     3361 tgaacatgcc atggtactgc cttatggttc cactatgcgt gcggttctgg aaaaagtccg
     3421 cccgaacagc atgtcgcaga tgaacgcggt tcagctttat cgcccatcag tggctcagcg
     3481 tcagaaagag atgctgaatc tctcgctgca aaaactggag gaagcatcac tttctgccca
     3541 gtcctccacc aaagaagaag ccagcctgcg aatgcaggaa gcacaactga tcagccgctt
     3601 tgtggcgaaa gcacgcaccg tggttccgaa aggtgaagtg atcctcaacg aatccaatat
     3661 tgattctgtt ctgcttgaag atggcgacgt catcaatatt ccggagaaaa catcgctggt
     3721 tatggttcat ggcgaagtgc tgttcccgaa cgcggtgagc tggcagaagg gtatgaccac
     3781 cgaggattac atcgagaaat gtggtggcct gacgcaaaaa tcgggtaacg ccagaattat
     3841 cgtcattcgt cagaacggtg ctgccgtcaa cgcggaagac gtggattcac tcaagccggg
     3901 cgatgagatt atggttctgc cgaaatatga atcgaaaaat attgaagtta cccgtggtat
     3961 ttccacaatc ctctatcagc tggcggtggg tgcaaaagtg attctgtctt tgtaaggagt
     4021 tgagatgagc aaagcagtta ttgtcatccc ggctcgctat ggctcctcgc gcctgccggg
     4081 taagccactg ctcgatattg ttggtaaacc gatgatccag catgtttacg agcgtgcgtt
     4141 acaggtggcg ggcgtcgcag aagtttgggt ggcaacagac gatcagcgtg ttgaaaaggc
     4201 cgtgcaggca tttggcggga aagccatcat gacgcgcaac gatcatgaat ccggcaccga
     4261 tcggctggtc gaggtgatgc ataaagtcga ggcagatatc tacattaacc tgcagggcga
     4321 cgaaccaatg attcgcccgc gggatgtaga aacgctgtta caaggaatgc gtgatgaccc
     4381 cgcgttgcca gtggcaacgc tatgccacgc gatttctgcc gaagaagcgg ctgagccaag
     4441 cacggtaaaa gtggtggtaa atacccacca ggatgcgctt tatttcagcc gttcgccgat
     4501 tccgtatccg cgtaatgctg aaaaagcgcg ctacctgaaa cacgttggta tttacgctta
     4561 tcgtcgcgat gtgctgcaaa actacagcca gttaccggag tccatgccag agcaggcaga
     4621 atcactggag cagctgcggt taatgagcgc ggggatcaac atccgcacat ttgaggttgc
     4681 cgcaaccggt ccgggcgtcg acaccccagc atgcctggaa aaagtgcgcg ccctgatggc
     4741 acaggaactg gctgaaaacg catgattggc attttctcgt ccggtatctg gcgtattccg
     4801 catctggaga aatttctggc gcaaccgtgc cagaaacttt ctctgctgcg ccctgttccg
     4861 caagaagttg atgctatcgc cgtgtgggga catcgtccca gcgcggcaaa accggtcgcc
     4921 atcgccaaag cagcgggaaa acccgtcatc cgtctggaag atggatttgt gcgttcgctg
     4981 gatcttagcg tcaatggtgc gccgccgctt tctctcgtgg tggatgattg tggcatttac
     5041 tacgatgcca gcaagccttc agcactggag aaactggtac aggataaagc cggaaataca
     5101 gctctgataa gtaaggccag agaggcgatg cacaccatcg tgaccgggga tttgtcgaaa
     5161 tataacctgg cgcctgcgtt tgtggctgat gagtcagaat gttcagacat cgttctggtt
     5221 gtcgatcaga catttaatga tatgtcagtg acgtatggta atgctggtca gaatgagttt
     5281 tctgccatgc tggaggccgc gatggcggaa aatcctcaag cgacaatttg ggtgaaggtg
     5341 cacccagatg tactgaaagg aaagaaaaca ggttatttcg ccgatctgcg cgccacgcaa
     5401 cgagtacgtt tgattacgga gaatatcagt ccgcagtcgc tgttgcgaca tatttccaga
     5461 gtttacgtcg tgacctccca atacggcttt gaggccttac tggcaggaaa accagtgaca
     5521 tgcttcggtc aaccctggta tgcaggctgg ggcttaacgg acgatcgtca tccgcagtcg
     5581 gctttgttat ctgcccgacg cggttctgtc acgctggaag aactttttgc cgcagcatac
     5641 ctgcgttact gtcgctatat cgacccacta acgggggcag caagtgacct gtttaccgta
     5701 ctgcaatggc tgcaattact gcgcaaccat cagcaacagc gtaatggcta tttatgggcg
     5761 ccaggcttaa cgctgtggaa gtcagcaatc ctgaaacctt tcttgcgtac cgcgacaaac
     5821 aggctgagtt attcacgtcg ctgtactgct gccaccgcct gcgtggtatg gggtgtaaaa
     5881 ggagaacagc aatggcaggt cgaagcgcag cgaaaatcac tgccattatg gcgaatggaa
     5941 gatggttttc tgcgttcatc cggacttggc tcggacctgc tgccatcgct atcgctggtg
     6001 ctggataaac gcggaattta ctatgacgcc acgcgcccca gcgacctgga agtgctgctt
     6061 aatcacagcc agttaacgct ggcgcagcag atgcgagctg agaagttgcg gcaacgactg
     6121 gttgaaagca aactgagtaa gtacaacctg ggagccgatt tctctctgcc tgccgaagcc
     6181 aaaggtaaaa aagttatcct ggtgccgggt caggtagagg acgattcctc tattaaaaca
     6241 ggcactatct caattaagag caaccttgag ttattacgca cagtacgcga gcgtaatccg
     6301 cacgcctaca ttatttataa accgcacccg gatgtactgg tggggaatcg caagggcgat
     6361 attccggcag aactgattgc tgaacttgct gattatcagg cactggacgc agatattatt
     6421 caatgcattc agcacgcaga tgaagtgcat accatgacgt cgctgtcggg gtttgaagcg
     6481 ttattacatg gtaagcacgt acattgttac ggcctgccct tctatgctgg ttggggttta
     6541 accttcgatg aacatcactg cgggcgtcgt gagcgagaat taacgttagc ggatttgcta
     6601 tatcaggcgc tgattgttta tccaacttat atccacccaa cacggctaca acctattact
     6661 gttgaagagg cagcggaata tttgatccag acgtcgcgca agccgatgtt tattacccga
     6721 aaaaaagcgg gatgggtaat acgctattac cgcaaattaa ttatgttctg caaggttaga
     6781 tttggctaaa caatttcata gttgactatt acgcaaatta tttatgcaag gtaatgcact
     6841 aaccgtttta ttatccggta aaaaatatct gctattgcag ggaccgatgg gacccttttt
     6901 cagtgatgta gccgagtggc tggagtcatt aggccgtaac gctgtgaatg ttgtattcaa
     6961 tggaggggat tgtttttact gtcgccaccg acattatcag gcttactacc agacaccgaa
     7021 agagtttccc ggttggttac gggatctcca ccggcaatat gactttgata ccatcctctg
     7081 ctttggtgac tgccgcccat tacacaagga agcaaaatat tgggcaaagt cgaaaggtat
     7141 ccgctttctg gcatttgagg aagggtattt acgcccgcag tttattaccg ttgaagaagg
     7201 cggagttaac gcatattcat cgctaccgcg cgatccggat ttttatcgta agttaccaga
     7261 tatgcctgcg ccgcacgttg agaacttaaa accttcaacg atgaaacgta taggtcatgc
     7321 gatgtggtat tacctgatgg gctggcatta ccgccatgag ttccctcgct accgccacca
     7381 taaatcgttt tccccctggt atgaagcacg ttgctgggtt cgttcatact ggcgcaagca
     7441 actttacaag gtaacacagc gtaaggtatt gccgaggtta atgaacgagt tggaccaact
     7501 ttattatctt gccgttttgc aggtatataa cgatagccag attcgtaacc acagtaatta
     7561 taacgatgtg cgtgactata ttaatgaagt catgtactca ttttcacgta aagcgccgaa
     7621 agaaagttat ttggtgatca aacatcatcc gatggatcgt ggtcacagac tctatcgacc
     7681 attaattaag cggttgagta aggaatatgg cttaggtggg agagtcattt atgtgcacga
     7741 tctcccgatg ccgggactat tacgccatgc aaaagcggtg gtgacaatta acagtacggt
     7801 gggaatctct gcactgattc ataacaaacc acttaaagtg atgggcaatg ccctctacga
     7861 catcaagggc ttgacatatc aagggcattt acaccagttc tggcaggctg attttaaacc
     7921 agatatgaaa ctgtttaaga agtttcgtgg atatatattg atgaaga
//
LOCUS       DAZFLD010000200         7770 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.201, whole genome shotgun sequence.
ACCESSION   DAZFLD010000200 DAZFLD010000000
VERSION     DAZFLD010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7770
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.201"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            79..1137
                     /locus_tag="ACHKBL_004002"
     CDS             79..1137
                     /locus_tag="ACHKBL_004002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HFO5820801.1"
                     /translation="MNKKIWFMEGLSSQRDIIQGVKLFAQKNNFAITVFASHRNERNE
                     ILSVADYSLTEPEDPQKRLQFIQETIQTYGIHHIHTGRNNQWFEEHRTAIESTGATLT
                     TGATGIDWLTLADEKVTFAQFMEQKGLPVVPSWRVNTLAELKTRLAAPPFTDSPVCVK
                     PVTGIYGMGFWRFDDSALPMAVFNHPEHRLVSPQLYIAAASASESFKPLVLMPYLPGP
                     EFSVDILADKGEILAAVGRRKEGAIQYLVNQGSAWELACDCARVMKADGLVNVQTRND
                     VNGNPVLLETNMRPSGGVGYTLHSGVNLPGLFAAFKLGLMSEDAVIQSARNTFSPVAV
                     RSITDVIVYPESLSNLLN"
     gene            1147..2289
                     /locus_tag="ACHKBL_004003"
     CDS             1147..2289
                     /locus_tag="ACHKBL_004003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase domain-containing
                     protein"
                     /protein_id="HFO5820802.1"
                     /translation="MSDTLEDMIYRRTLSCGTIQVTRDQGEVSLDDLFDIAERRNPKR
                     AFLFVSKVLGRHIPVPPSVMRQAYRQLASQFPSTLTGPILFIGMAETAVGLGAGVFDE
                     VRHQHRESVYLTSTRHPVDGTLLCEFKEEHSHATDHLIYLPDDEEKRRRVTNARTLVL
                     IDDEATTGNTFINLLSALRNTGKLQHIEQVIAVTLTDWSENALSRRSTLPVTSVSLVS
                     GQWGWTPLPDAPVPDMPKVNVTSRGKWDIQGKQSWGRLGMLAPAADLGHEVSFRKGER
                     VLVLGTGEFVWEPFLLAERLEAAGAQAFYGATTRSPIAVGYAIKSAISFTDNYGLGIP
                     NFVYNVAHQQFDRILVCTETPAESIDTRLLKALAEVAPVVEIVTYE"
     gene            2282..3055
                     /locus_tag="ACHKBL_004004"
     CDS             2282..3055
                     /locus_tag="ACHKBL_004004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5820803.1"
                     /translation="MNKPVVLSDLDDTLFQTRRKMVDELALEPFRTGAVDRTLNPRSF
                     MTEEQSMLVDWLLEQAELIPVTARGTEEISRVRIPFHSWAITTHGAVILTPEGKPDEE
                     WKAHMLGQLTPYQEKLASMQRLITEMMDAKGINAWARLNFEYGETAVYMVMKHRDSTR
                     LDELNAIADEIETVFSTEGFYIHRNSNNVAWLPTPVEKGLAVSWLLEKLRAERGVFPV
                     IGLGDSLSDHRFMKLCSWFGIPRQSQFADAISQRIFGEN"
     gene            3057..4136
                     /locus_tag="ACHKBL_004005"
     CDS             3057..4136
                     /locus_tag="ACHKBL_004005"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine protease StiP family protein"
                     /protein_id="HFO5820804.1"
                     /translation="MQPHETFTGSYQPGDVEFLLKPVVIEMTPVDQKEELIQSGKKHY
                     SDMLSQEPAPTQWHLDLFHRALDRGAERLAKEVTQLAIALAERFGDEPIVLASLVRAG
                     VPLGVMLHQALRDMGKTSWHYGISIIRDRGIDGTALDIIEERHGTSGIVFVDGWTGKG
                     AITGELVRALRDRPGYPEQPRLVVLADPCGCSWLAASDDDWLIPFGIMGAPVSGLLSR
                     SVWSSEGLHGCMVCEHLSEFECSRMLVDTVAHFRKKLTPSSLAPLSWNTESTRVLWQT
                     SRDVIAFLADEFKVDSVNRIKPGIAEATRAVLRRVPDHVFVRSIDDPDVALLVGLARE
                     KGIIVTEMGGTLGQYRAVTIIKKVF"
     gene            4136..5092
                     /locus_tag="ACHKBL_004006"
     CDS             4136..5092
                     /locus_tag="ACHKBL_004006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HpcH/HpaI aldolase/citrate lyase family protein"
                     /protein_id="HFO5820805.1"
                     /translation="MKNRLSPWNLGATLYMPATREDIADAVLHEKIPGLRSLVICLED
                     AVSEADIPVALKNLEHLLHELSNSMRSLGKNNWPLLFIRPRHAEMGKWLKEHYDLSAV
                     DGFVLPKFTLSSLAEWWDIMGGTHLSMMPTLETENVFDVIQMRELATRLLEHPCHKRI
                     IALRIGGNDLMNVVSLRRPRALTLYDSPMGYVIKMLVSVFSPRDFALTAPVCEHIDDH
                     AVMARELALDMAHGLVGKTAIHPDQIEVIQNALMVTQGEYSDALRILNSTQAVFKSQG
                     AMCEPATHRRWAAGILDRARFYGLHNEQSADGIRLLTITQHH"
     gene            5103..6198
                     /locus_tag="ACHKBL_004007"
                     /pseudo
     CDS             5103..6198
                     /locus_tag="ACHKBL_004007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309371.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            6216..6683
                     /gene="terW"
                     /locus_tag="ACHKBL_004008"
     CDS             6216..6683
                     /gene="terW"
                     /locus_tag="ACHKBL_004008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurium resistance protein TerW"
                     /protein_id="HFO5820806.1"
                     /translation="MQLNTRQARIFKLANLLGSGKPVSATDIITYLDCSEPTLTRALK
                     ELRKSYSAEIKYSKAGHSYHLVNPGQLDKKTLRRMNEALAQNADLKTGESTGKVVLDK
                     DKKTAVSLSLRMRILRKIDRLAALSGSTRSEAVEKLALHSVDELIKEYSAKKS"
     gene            7052..7705
                     /locus_tag="ACHKBL_004009"
                     /pseudo
     CDS             7052..7705
                     /locus_tag="ACHKBL_004009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014543172.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
BASE COUNT         1969 a         1934 c         1981 g         1886 t
ORIGIN      
        1 atgattgaca tgtagcatat acccccataa actcacttct cgacaataaa ttttcttgcc
       61 gttccggata ataaaaaaat gaacaaaaaa atttggttta tggaaggttt gtcctctcag
      121 agagatatta ttcagggggt taaattattt gcacaaaaaa ataattttgc cattaccgtt
      181 tttgcctccc accgtaacga aagaaatgaa atcctttccg ttgccgatta ttctttgact
      241 gaacctgaag atcctcaaaa acgtcttcag tttattcagg aaaccattca gacctacggc
      301 atccaccata ttcatactgg tcgtaacaac cagtggtttg aagaacaccg aaccgccatt
      361 gaatcgaccg gtgccaccct tactaccggt gcaacgggca tcgactggtt aactctggct
      421 gacgaaaaag ttacttttgc tcagtttatg gagcaaaagg ggctcccggt cgtaccatcc
      481 tggcgggtga atacgctggc agaattaaag acacgcctcg cggctccgcc gttcactgac
      541 agtccggtat gcgtgaagcc ggtgacgggt atctacggca tgggattctg gcgctttgat
      601 gacagtgctt tgcctatggc cgtctttaat caccccgaac atcgtctggt cagcccgcaa
      661 ctgtatattg ctgcagcgtc ggcttctgag tcgtttaaac cccttgtttt gatgccgtac
      721 ctgcctggcc cggaattttc cgtcgatatc ctcgcggata agggcgaaat actcgcagcc
      781 gtgggacgcc gtaaggaagg ggctatccag tatctggtaa accaaggaag cgcctgggaa
      841 ctggcgtgtg actgcgcccg ggttatgaag gccgacgggc tggtgaatgt tcagacgcga
      901 aacgatgtga atggcaaccc ggtgctgctt gaaaccaaca tgcgtccgtc aggcggggtg
      961 ggttataccc ttcacagcgg ggttaacctt cccgggttgt ttgctgcttt taagctcggt
     1021 ctgatgtctg aagatgcggt gatccagagc gccagaaata ccttttctcc ggttgcagtg
     1081 agatccatca cagatgtcat cgtttaccca gaatcactct ctaaccttct gaattaaggc
     1141 gtgtttatgt ctgataccct cgaagatatg atttatcgcc gtaccctctc atgcggtacg
     1201 attcaggtaa cccgcgacca gggtgaagtt tcgctcgatg acctgtttga tatcgctgaa
     1261 agacgtaacc cgaaacgcgc ctttctgttt gtcagcaaag tactcggcag gcacattccc
     1321 gtgccaccct cagtcatgcg gcaggcctac agacagcttg ccagccagtt cccctcaacg
     1381 ctgacagggc ccatactgtt tatcggtatg gcagaaaccg ccgttgggct tggggctggt
     1441 gtgtttgatg aagtgcgcca ccagcatcgt gaatctgtct atctgacctc tacccgtcac
     1501 ccggttgatg gcacattgct ttgcgagttc aaggaagaac acagccacgc caccgatcat
     1561 ctgatctatc tgccagacga tgaagagaaa agacgtcgtg ttacaaacgc gcgaactctg
     1621 gttttgattg atgacgaagc aaccaccggt aataccttta ttaacctgct ttctgccctg
     1681 cgtaataccg gcaagcttca acatattgaa caggttatcg ctgttacgct tacggactgg
     1741 agcgaaaatg ccctgtccag gcgcagcact ttacccgtca cttcggtttc tcttgtaagt
     1801 ggtcagtggg gatggactcc attaccagat gcacctgtcc cggacatgcc gaaggtcaac
     1861 gtaacttcac gtggaaaatg ggacatccag ggaaaacagt cctggggtcg actggggatg
     1921 ctcgcacctg cggccgatct cggtcatgag gtatccttcc gcaaggggga gcgtgttctg
     1981 gttctcggga caggggaatt cgtctgggag ccgttcctgc ttgctgaacg gctcgaagct
     2041 gccggagcac aggcatttta tggtgcgacc acccgctccc ctatcgccgt tggttatgcc
     2101 attaagtccg caatttcctt tacggataac tacggcctgg gcatccccaa ttttgtctat
     2161 aacgtcgccc atcagcaatt tgaccgcatt cttgtgtgta ctgagacacc tgcagaaagt
     2221 attgacacgc ggcttcttaa agcgctggct gaggttgcac ccgtcgtgga gattgttacc
     2281 tatgaataaa cccgttgttc tgagcgacct tgacgacacg ctctttcaga cccgtcgtaa
     2341 aatggtagac gagctggccc tggagccatt ccgtaccggt gccgttgatc gtactctgaa
     2401 tccacgaagc tttatgacag aagaacagtc catgttagtg gactggctcc tagagcaggc
     2461 tgaactcatt cccgttaccg cacggggaac tgaagaaatc agccgcgtaa ggatcccttt
     2521 ccactcctgg gcaatcacca cgcacggggc cgtcattctc acgcctgaag gcaaacccga
     2581 cgaggagtgg aaggcccata tgctcggcca gctaactccc taccaggaaa agctggcatc
     2641 gatgcagcgt ctgatcactg aaatgatgga cgcaaaaggg atcaatgcct gggcaagact
     2701 gaacttcgaa tacggtgaaa cggcggttta tatggtgatg aaacaccgcg acagcactcg
     2761 ccttgacgaa ctcaatgcca ttgcagatga gatagaaacg gtgttttcga ccgagggctt
     2821 ctacatccac cgcaacagta acaacgtagc ctggcttccc accccggttg agaaagggct
     2881 ggccgtcagc tggctgcttg aaaaacttcg ggctgaacgc ggagttttcc ccgtaattgg
     2941 tctgggcgac agcctgagcg atcatcgttt tatgaaactg tgcagctggt ttggcatccc
     3001 gcgtcagagt cagtttgcag atgccatttc acagcgaatt tttggagaaa attaaaatgc
     3061 agccacatga aacatttacc ggctcatacc agcccggtga cgtggaattt ctgctaaagc
     3121 cggttgtcat tgaaatgacg ccagttgatc aaaaagaaga gctgattcag tccgggaaga
     3181 aacattattc ggacatgctc agccaggagc cagcgccgac acaatggcat cttgatttgt
     3241 ttcaccgggc gctggatcgg ggcgcagaga gacttgcaaa agaagtcaca cagctcgcta
     3301 ttgctctggc cgaacgcttc ggtgatgagc ccatagtact ggccagtctt gtcagggctg
     3361 gcgtaccgct cggcgttatg ctacaccagg ctctgcgtga catgggaaaa acctcatggc
     3421 attacggcat cagcatcatc cgggatcgtg gaattgacgg tacagcactt gatatcattg
     3481 aagagcggca tggtaccagc ggcattgtct ttgttgacgg atggacaggt aaaggcgcaa
     3541 ttaccggaga gcttgtacgc gcactgagag atcgcccggg ctatcctgag cagccgcgac
     3601 tggttgtcct ggccgatccc tgtggctgtt cctggcttgc agcaagtgat gatgactggc
     3661 tcattccttt tggcatcatg ggggctccgg tgtcaggcct gctctcccgg tcagtatggt
     3721 cctctgaagg attacatggg tgcatggttt gcgagcatct cagtgaattc gaatgcagcc
     3781 ggatgcttgt cgataccgtc gctcatttcc gtaagaagtt aacaccgtca tccctcgctc
     3841 ctctgagctg gaatacggag tcaacccggg tcttatggca gacaagtcgc gacgttatcg
     3901 cgttcctggc cgatgaattt aaagtggaca gcgtcaaccg tattaaaccc ggtattgctg
     3961 aagcaacccg ggctgtatta cgtcgggtac cggaccatgt atttgtgcgt tcgattgacg
     4021 acccggacgt tgccttactt gtggggcttg ctcgtgaaaa gggaataatt gttacagaaa
     4081 tggggggaac cctcggccag tatcgggctg taaccattat caaaaaggta ttctaatgaa
     4141 aaatcgtctt tctccctgga atcttggtgc cactctatac atgcctgcaa cacgggaaga
     4201 tattgctgat gccgttctgc acgagaaaat cccgggtttg cgctctctgg tgatttgcct
     4261 ggaggatgct gtcagtgaag cagacatccc cgtcgccctc aaaaatctag agcacctgct
     4321 gcacgaactc agtaacagca tgcgtagcct gggtaaaaat aactggcccc tgttgttcat
     4381 tcgcccccgg catgccgaaa tgggcaaatg gttaaaagaa cattatgatc tttccgctgt
     4441 cgacgggttt gtgttaccga aatttaccct aagttcgtta gctgaatggt gggatatcat
     4501 gggcggaact cacctgagca tgatgccaac gctggaaaca gaaaacgtct ttgatgtgat
     4561 tcagatgcgc gagctggcca cccggctgtt ggaacatccc tgccacaagc gcattattgc
     4621 gcttcgaatc ggcggcaacg atcttatgaa cgtcgtgtcg cttcgccgtc cccgagccct
     4681 gacgctgtat gacagtccga tgggctacgt catcaaaatg ctcgtttcag tcttcagccc
     4741 gcgtgatttt gccctgaccg cgcccgtatg tgagcatatt gacgatcatg ccgttatggc
     4801 cagagagctg gctcttgata tggcacatgg acttgttgga aaaacggcca ttcatccaga
     4861 tcagatagag gtcattcaaa acgcgctgat ggtcactcag ggtgagtact ctgacgctct
     4921 gcggatcctg aactctaccc aggccgtgtt taagtcgcag ggggcgatgt gtgaacctgc
     4981 cacacatcgc cgctgggcgg ctggcattct ggacagagcc cggttttacg ggttacataa
     5041 cgagcaaagc gctgatggaa tcagattact tactataacc cagcatcatt aaggaaatca
     5101 taatggggtt caggttcaga ggtacttccg tttctttctg tagccggggg gcctatgcca
     5161 atctggggtt acccggtacc gagctgagtt accgtacccg gtttgagctg gccgcgcctt
     5221 ccggaggtgg aaacaggaca gcaaccgtcc cggatctcag acagtcgctt gagcaggaag
     5281 cagttgaact catgtcagcg gtgaccgcaa ttcgtaacat tcacgagttg acgctggatc
     5341 caaaaacagg catcagttgg gcagagctgg aagcagtata cctgcatagc agaacatcgc
     5401 tttttcaggt tccggcacca gtgcgtcctg aaaaaccaga ataccatgca ttgtcggaaa
     5461 atcctgcagg gaacaaaggc attgtttttc tgggtaaatg gtttgaatcg gaatcagcta
     5521 aagctgagcg tcaagcaggg aatcttcgcc gatggcggca agaactgatt gatgtgaagc
     5581 gtgagaatcc ccttcgacag caccggtacc agcaacggca gacgaccagg gccgaacagt
     5641 attcaaactg gaagtgtgat gctgaagaac atgaaaaaag gctcgccacg actcaggcag
     5701 atgcccggca gttgttccgg gcagacgcag cgtttttcga atcatacctt gcgggtgtgc
     5761 tttcagaaac tgaatggccg cgtgaaacgc ttgttgcatt tgaagtaaag ccggagatat
     5821 caacagtcct gccggatgtt ggtttagctg agattgaaga tttccctgat aagatttacg
     5881 gcgtcaatgc ccggggcatg gagctgacgc aaaaagccat acgcgaaaac tatgcccgcc
     5941 acagccatgg ctgcttgttc cgactggtcg gtatcgtgtt acatacgcta cctttcgaca
     6001 aggtgatcgc atcaggattt acacaacgag taagcggaac gactatctgg aggatgttta
     6061 tatcctgtcc tgcaaatgca ctcgcagcca gaaggcatag aacatattga tccggttgaa
     6121 gcgttaggct atcaccctgt tattcgaaag atgagaaaca cattcatttt tcagcctatt
     6181 gaaccactaa ccctttaagc aaactaaata ttacgatgca attaaatacc agacaggcca
     6241 gaatttttaa gcttgccaac ttactgggct ccggcaagcc tgtttccgct accgacatta
     6301 taacctatct ggactgctca gagccgacat taacacgggc cctcaaagag ctacgaaagt
     6361 catattctgc agaaatcaaa tacagtaagg caggtcattc ctatcatctc gttaatccgg
     6421 gtcaactgga taaaaagaca cttcgccgga tgaatgaggc gcttgcacag aatgctgatc
     6481 tgaaaaccgg tgagtctaca ggaaaggtcg tactcgataa ggataaaaaa acagccgtat
     6541 cattatcgct acgcatgcga attttaagaa aaattgaccg tctggctgca ctgagtggat
     6601 cgacccgaag tgaagctgtt gaaaaactgg cactgcattc cgttgacgaa ctgataaaag
     6661 aatacagcgc taaaaaatcc tgatgacaaa tctcagctcg gggtgaatac cccggttttt
     6721 tcatatttca actctgtctt ggcgtgacgt atttttgatc tattctcctc ccgcaatgtc
     6781 agcagctcct tacagcctac aaaacctagc gtcttgacat cctcttgtgc tacgcatcaa
     6841 tctttgggcg atttacactc cactcatgca aatctgtgat gtatgatgat tttcagtaag
     6901 tgtaaactga ctgccgtatg tagccattaa gccagtattg gtaacgtaga tgcccacctt
     6961 ttcacttatt tggtggacac cactttgtct aactcgtcag attctgacca aacgtttcag
     7021 gctgtacgct tactctgaag gggggagaat agtagcccgt gatagcattg agctaactga
     7081 cagccagatc gcagcactaa agcgtaaagc cagtgatgac gaggcctgtg gcgaaagcga
     7141 aacggctcat ccgggatatc tgggttcgca ggacacgttc tatgtaggca acctgaaagg
     7201 tgtcgggcgt atttatcagc agacgttcgt tgatacgtac tcgaaagtgg ctcactgtaa
     7261 gctttacgtc accaaaacac cgattaccgc ggctgactta ctgaatgatc gtgtactgcc
     7321 gttttatacg tctcagggcc tgccgatgct gagaatactg accgacagag gcacggagta
     7381 ctgtggcaaa gtagagcagc atgattatca gctttatctg gcaataaacg atatagaaca
     7441 cacgaaaaca aaggcgatgt ccccgcagac caacggcatc tgcgagcggt tccataaaac
     7501 aatcctgaac gaattttatc aggtaacgtt ccgtaaaaaa ctgtacggcg atcctgatac
     7561 attacagtcg gatcttgatg aatggctggc tcactataat aatgagcgaa gccatcagag
     7621 aaaaatgtgc aatgggcgga cgccaatgga aacgttactt gatggaaagc gcatctgggc
     7681 cgagaaaaat ttaagccaga tgtaatctga cagatacctg tataaataac cggtaactgt
     7741 cagatcaggt ctgagctaat acaaattaag
//
LOCUS       DAZFLD010000201         7733 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.202, whole genome shotgun sequence.
ACCESSION   DAZFLD010000201 DAZFLD010000000
VERSION     DAZFLD010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7733
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.202"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..162)
                     /locus_tag="ACHKBL_004010"
     CDS             complement(<1..162)
                     /locus_tag="ACHKBL_004010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000730426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein"
                     /protein_id="HFO5820807.1"
                     /translation="MQKKSLTLLLPSLLSGCTAFNATPVPDTTPPALVFVDGQIRESA
                     AIIAHTQKRL"
     gene            420..704
                     /locus_tag="ACHKBL_004011"
     CDS             420..704
                     /locus_tag="ACHKBL_004011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001532124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5820808.1"
                     /translation="MNMCDSKYTLISGEGGLRADCADYLFFAVYAIALLMIILWMTAS
                     VIYSIEEYTSQEKYGKSKVYSYFWFFIWFFVFNCAVYSSLKAINIKDIIS"
     gene            complement(697..1584)
                     /locus_tag="ACHKBL_004012"
     CDS             complement(697..1584)
                     /locus_tag="ACHKBL_004012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5820809.1"
                     /translation="MSTSSFIFAILPGILMICIITSLFWVVIYARGKWTFSSGITVFR
                     YLQQYRWFHFGNISLLSLSVILTTRVYDTEYPLQVYVCIYIPPVMILTSLLTILTIKE
                     ATRTYKTYKSNRLTPVSVIAYAGLGYMFCFTTGIYELSSTRLNPVISDMLMSFLSRLS
                     IMLPVTLAEIAVIYLLLLALHFPDGVSARGFSVTRYLRHNDWFYYINTGNAILALILS
                     TWIYHSSISLPAALFTGALSIFFNTTIPASLSVIEHTPSDTDSKIWDTRKTSPATVLY
                     CSGTGYVIGFIVNPSAAIS"
     gene            complement(1777..2019)
                     /locus_tag="ACHKBL_004013"
     CDS             complement(1777..2019)
                     /locus_tag="ACHKBL_004013"
                     /inference="COORDINATES: protein motif:HMM:NF022090.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilI type IV pilus biogenesis protein"
                     /protein_id="HFO5820810.1"
                     /translation="MSAEKFRVVIYTNTCEKKVSYIPATEDINIAIMPFQTADNGLII
                     VCQNSRIIRRKERIPGSSCEWKDVTLAPKSRNRVQL"
     gene            complement(2100..3794)
                     /locus_tag="ACHKBL_004014"
     CDS             complement(2100..3794)
                     /locus_tag="ACHKBL_004014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000482663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HFO5820811.1"
                     /translation="MFNLPGWLFTVIALVLTLFVIGLIFARLYRRASAEQAFVRTGLG
                     GQKVVMSGGAIVMPIFHETIPVNMNTLKLEVSRAAAESLITRDRMRVDVAVAFFLRVK
                     PSAEGISTAAQTLGQRTLTPEDLRSLVEDKFVDALRATAARMSMQDLQDARENFVQGV
                     QNTVAEDLSKNGLELESVSLTSFNQTAREHFNPDNAFDAEGLTLLTQETERRRRERNE
                     VEQDVEVAIREKNRDALSRRLEIEQQEAFMTLEQQQRVKTRTAEQSASIAAIEAERRR
                     EAESARILAERKIEEAEIERQQIVRTRQVEAEREVAIREIEQQQATEIASQARAIAVA
                     AKSEEQSQAEARASKALAEAVQAQQDVETTRQTAEADRTKQVALIAAAQEAETKAVEL
                     TVRAQAEKEAAELQAAAIIELAEATRQKGLAEAEAQRALNDAINVLSDEQTSLRFRLA
                     LLDALPAVIEKSTEPMKSIEGIKIIQVDGLNGGVTRGKTATTDISGGNLAEQAVTAAL
                     AYRTQAPLIDSLLKEVGLSGEGLTALTRPLVMPAPEKVPDVSEPVTAPDNSEPQQV"
     gene            complement(3821..4441)
                     /locus_tag="ACHKBL_004015"
     CDS             complement(3821..4441)
                     /locus_tag="ACHKBL_004015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000176498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold-containig protein"
                     /protein_id="HFO5820812.1"
                     /translation="MTLTLLTAYNAPYLAALTFVLLTGIAELIALLCGHSLSSAMDTP
                     DLPEGLTGEALDWLNIGRIPLLIVLCMLAVFFGISGILLQGLIIHLLQAPAPNILLAP
                     LCLLLTCPLVHRTGRLIAPWLPRDETTALTEEAFIGCMATITGYHAVAGSPCEGRFTD
                     RFGQVHYLLLEPEAGKEFRRGDKVIIVCRLSGSRWLGELNPWPDVL"
     gene            complement(4707..5372)
                     /locus_tag="ACHKBL_004016"
     CDS             complement(4707..5372)
                     /locus_tag="ACHKBL_004016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000154135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="traC"
                     /protein_id="HFO5820813.1"
                     /translation="MTILSQENPVILLLENMLEALSTAPDNINNERRRRRYLLNWLDT
                     ARQMREFRGMAEEFTTLRKLLATNSDIPVANTLKSLLESGNNAALYDLYRFRSALSAC
                     RSLGWQIGTCAWNDQLLSETLSRAQTGKRHILQLNSTRDTFSTTGRMLRPVAFSLIHP
                     ASLESSEVEEIFRDEGFILAHGRECSLNGSGRNMLSRILHVGHSGLPKAEWGIDHSNH
                     LLH"
     gene            complement(5513..6154)
                     /locus_tag="ACHKBL_004017"
     CDS             complement(5513..6154)
                     /locus_tag="ACHKBL_004017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000335849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006354 - DNA-templated transcription
                     elongation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0140673 - transcription elongation-coupled
                     chromatin remodeling [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination NusG
                     family protein"
                     /protein_id="HFO5820814.1"
                     /translation="MCLSELQAGLNEGQPLFPTHALCIRRSQQHRSPSAGIDFRGAFR
                     ILNISELHQRNWYLAQYIPAGKNREHLFSWLSEQHVLPWTPLILKKVRRTDKVCGYRR
                     HIHAVFPGYFFLKADPERHSFTHLRRHSAFLNFVKMAGEIKTVREDIVQSLMKVYPDP
                     ALNPAAREELDAASTLWLTKARYQYLLRLDAQPLPESRIALLLHLVSDDGALT"
     gene            6857..7729
                     /gene="repA"
                     /locus_tag="ACHKBL_004018"
     CDS             6857..7729
                     /gene="repA"
                     /locus_tag="ACHKBL_004018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HFO5820815.1"
                     /translation="MAALNQTSSTESTYRQVKNPNPVFTPREDAGTLKFCEKLMEKAV
                     GFTSRFDFAVYVAYARSRGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTL
                     AIECGLATESAAGKLSITRATRALTFLSELGLITYQTEYDPLIGCYIPTDITFTPALF
                     AALDISEEAVASARRSRVEWENRQRKKQGLDALGMDELIARAWRFVRERFRSYQAELK
                     SHGMKRARARRDAGRTRQDIVTLVKRQLTREIAEGRFRGSLEAVKREIDRRVKERMIM
                     SRNNNYTRLATASP"
BASE COUNT         1959 a         1774 c         2041 g         1959 t
ORIGIN      
        1 cagacgtttt tgcgtatggg cgataatggc agcgctttcg cgaatttgtc cgtcgacgaa
       61 caccagagcc ggaggtgtgg tgtcaggaac aggcgtggca ttaaatgccg tacagccgct
      121 gagtaatgac ggcagaagga gtgtaagaga ttttttttgc atagaataaa atccggtata
      181 ttacgggtta cggtataaga caggtggcgg gtaactggcc ttcagataaa tataatttta
      241 tcaggatatt gatgatgaac attcatgttc ggggtgatta tgaaaactaa aacattccgg
      301 ttccggaaat ataaaagaaa aacgtcctgc tgcaaatgat agtttccaga aaacaagtga
      361 tattgttacg caatattatg acggactctt tattattttg ctcggtaggt tttgtgttta
      421 tgaatatgtg tgattcaaaa tatacgttga tttctggtga gggtggttta cgtgctgatt
      481 gtgccgatta tttattcttt gccgtatatg caattgcgtt gctaatgata attttatgga
      541 tgacagcttc agttatttat tctattgaag aatatacgag tcaggaaaaa tacggtaaaa
      601 gtaaagtcta tagctacttc tggtttttta tctggttttt tgtttttaac tgtgctgttt
      661 attcttctct gaaggcaata aatataaaag atataatcag ctaatggcag cagagggatt
      721 cacgataaaa ccaatgacgt aacctgttcc cgagcaataa aggactgttg ccggggatgt
      781 ttttcgtgta tcccatatct ttgaatctgt atcagatggc gtgtgttcaa tgaccgataa
      841 gctggccgga atggtcgtgt taaaaaaaat acttaacgcg cctgtaaata aagcagcggg
      901 aagagaaatg ctggaatgat atatccatgt ggagagtatc agtgccagaa tagcatttcc
      961 tgtatttata taataaaacc agtcgttatg gcgcagatac cgggtaacgg agaaccccct
     1021 ggcggacacg ccatccggga aatgcagcgc cagaagcagg agataaataa ctgctatttc
     1081 agccagtgta acaggcagca taatggaaag acgtgacagg aatgacatca gcatgtcact
     1141 tatgacagga ttaagtcgtg ttgaagataa ttcgtaaatg ccggttgtaa aacagaacat
     1201 ataacccagt ccggcataag caataacgga aaccggcgtc agtctgtttg atttgtatgt
     1261 tttatacgtt ctcgttgctt ctttaatggt taaaattgtc agcagggatg tgagaatcat
     1321 aacgggtggt atatatatgc agacataaac ctgcaaagga tactcagtat cgtatacccg
     1381 ggttgttaat ataacggaca gactcagtaa ggatatattt ccgaaatgaa accagcgata
     1441 ctgttgaaga taccggaaaa cagtgatgcc tgaagaaaat gtccatttgc cgcgagcata
     1501 aataacaacc cagaataaag aagtaatgat acatatcatt agtatgccgg gtaatattgc
     1561 aaaaataaat gatgatgtgc tcattattaa atctccatca gcatggtgat gcttaaattt
     1621 ttacggttgt gaggtgcgta atcaatatca tgtgtagttg tgaataatag cgtcagcaac
     1681 atatataaaa ataatctgca ttcctgaaaa atatcgcggt tgtgaaatat ttaattttta
     1741 ccccctttta tgggggatga aaagcaggct gtcaggctat aactgaacgc ggtttctgga
     1801 ttttggtgct aatgtcacat ctttccattc acagctgctt ccggggatac gctctttgcg
     1861 tcgtattata cgggagttct gacacacaat aattaagcca ttgtctgccg tctgaaatgg
     1921 cattatagcg atgtttatat cttctgttgc aggaatataa ctgactttct tttcacatgt
     1981 attggtgtat atgacaactc tgaatttttc tgcggacatt ttcaatcctc ttttaaacca
     2041 gaatatcccg tgagggcatt ctttgtgaaa aagatgccgg aaaaattccg gcacggggat
     2101 taaacctgtt gtggttctga gttatccgga gcggttaccg gttcgctgac atccggaacc
     2161 ttttccgggg ctggcatgac cagtggacgt gtcagggctg taagtccttc accggataac
     2221 cccacctcct tcagcaggga gtcgatgagc ggagcctgtg tacggtaagc cagagcggcg
     2281 gttaccgcct gttcagcgag atttccgcca cttatgtcgg ttgttgctgt tttaccacgg
     2341 gtaacaccgc cattcaggcc atccacctga atgattttaa tgccttcaat ggatttcatt
     2401 ggttctgtgg acttctctat gacggcaggt aatgcatcca gcagggcaag acggaaccgg
     2461 aggctggtct gctcatctga aagcacgtta atggcatcgt tcagtgcgcg ctgtgcttcc
     2521 gcttctgcca gacctttctg acgggtggcc tccgccagtt caataattgc tgctgcctgg
     2581 agttcggctg cttctttttc agcctgggcg cgaaccgtca gttcaaccgc tttcgtttca
     2641 gcttcctgag cagcagcaat cagggcaacc tgtttggtac ggtcggcctc cgcagtctgg
     2701 cgggtggttt ccacatcctg ctgggcctgt acggcttcag ccagtgcttt acttgcccgg
     2761 gcttctgcct gtgactgctc ttccgatttt gccgccacgg caatggctct ggcctggctg
     2821 gcgatttcgg ttgcctgctg ttgctcaatt tcccgtatgg ccacttcccg ttcggcttcg
     2881 acctggcggg tacgcactat ctgctgtcgt tcgatttcgg cttcctctat cttacgttca
     2941 gccagaattc gcgcggattc tgcttcacga cgtcgttcag cttcaatagc agcaatactg
     3001 gcactctgct ctgccgtacg ggtttttacc cgttgttgtt gctccagagt catgaaggct
     3061 tcctgctgtt caatctccag acgtcgggag agtgcatccc ggtttttttc cctgatggcc
     3121 acttcaacat cctgctcaac ttcgttacgt tcgcggcgac gacgttctgt ttcctgggtc
     3181 agaagggtca ggccttcagc atcaaaggca ttgtcaggat taaaatgttc ccgtgcagtc
     3241 tggttgaaac ttgtcagcga cacactttca agctccagac cgtttttgga taagtcttcc
     3301 gcaacggtgt tctgcacacc ctgaacaaag ttctcgcggg catcctgtaa atcctgcatg
     3361 ctcattctgg ccgccgtggc ccgcagggca tcaacaaatt tatcctcgac cagactacgc
     3421 agatcttcag gtgtgagcgt gcgttgtccc agcgtctggg ctgcagtgga tattccttcg
     3481 gctgatggtt ttacccgaag aaaaaaggca acggcaacat caacacgcat gcggtcgcgg
     3541 gtaatgagac tttcagccgc tgcacgactg acttccagct tcagggtatt catgttgacg
     3601 ggaattgttt cgtgaaaaat gggcataaca attgcaccgc cactcattac cactttttgg
     3661 cctcccagac ctgtgcggac aaaggcctgc tctgccgatg cccgacggta caggcgggca
     3721 aatatcagcc cgataacgaa cagggtgagg accagcgcaa taacggtaaa caaccatccc
     3781 ggaagattaa acatagtgag aacctctgta tatgtcggga ttaaagaaca tccggccagg
     3841 gattcagttc acccagccag cgggaaccgg aaagacggca gacaataatc actttgtctc
     3901 ctcgtctgaa ttctttcccg gcctccggct ccagcaacag gtaatgaacc tgcccgaagc
     3961 ggtctgtgaa gcgaccttca cagggggaac ccgccactgc gtgataaccg gtgatggtgg
     4021 ccatacagcc gatgaatgcc tcctcggtga gcgccgttgt ttcatcacgg ggtaaccagg
     4081 gggcaatcag tctgccggtg cggtgtacga gaggacatgt cagcagcagg caaagtggtg
     4141 caaggagaat gttcggggca ggagcctgca gcagatgaat aataagaccc tgcaggagaa
     4201 tgccggatat accaaaaaaa actgccagca tacagaggac gatcagcagg gggatccgtc
     4261 cgatgttcag ccagtccagc gcctctcctg tcagaccctc cggtaagtca ggtgtatcca
     4321 tagctgaaga gagggagtgt ccgcagagca gggcgataag ttcagcgata ccagtaagca
     4381 gaacaaaagt cagggctgcc agatagggtg cgttatacgc ggtaagtaac gtcagtgtca
     4441 tttttcctcc tgtggcaggt cagtcaaaca acattatgcg tgaagcttcc gcttctttaa
     4501 ttgtcagaag aagcatatcg tcagtcatca gaagacaagg ggatgtctgc aggaggggat
     4561 gataacaaca gaagcctcac gccggaatgt gctttatgac aacaggagag gacggataat
     4621 aaaaaatggg acatccacat cagcaaaccg cgatttgcga aaaaaagtca gcggagagtg
     4681 gctgccgttg tggctgtccc ggtatttcag tgtaacaggt gattgctgtg atctatgccc
     4741 cattcggctt tcggtaaacc tgaatgcccc acatgaagaa tgcgtgacag catgtttctg
     4801 ccgctgccgt tcaggctgca ttcgcgtccg tgcgcgagta taaaaccttc atcccggaat
     4861 atctcttcca cttctgaaga ttcgagggat gccggatgaa ttaatgaaaa tgccaccggt
     4921 ctgagcattc tgcccgtggt ggaaaaggtg tctctggtac tgttcagctg caggatatga
     4981 cgtttacctg tttgcgcacg ggaaagtgtc tcactcagca gctggtcgtt ccatgcgcac
     5041 gtgccaatct gccatcccag acttctgcac gcggaaagag ccgagcggaa gcggtacagg
     5101 tcgtataatg ctgcgttatt gccactttcc agcagtgatt tcagggtgtt tgctaccggt
     5161 atgtctgaat tggtggccag cagttttcgc agggtagtga actcttcagc cattcctctg
     5221 aactcgcgca tctgacgggc agtgtcaagc cagttcagca ggtaacgccg gcgacgccgt
     5281 tcgttgttta tgttgtccgg ggcggtggag agtgcttcga gcatattctc cagtaacaga
     5341 atgacagggt tttcctggga tagaatggtc ataaaggcgt cctgaaaaaa tgcccgacag
     5401 ccgctctccg tacaccctca ggtgtcaccg gtgccgggca ttgtggaaac agaagggaaa
     5461 aaaatcccgg ccataaaggc cgggaaaagt cgtcaaatta agacagcaat tttcatgtca
     5521 acgctccgtc atcagatacc aggtgcagga gcagggcgat cctcgactcc ggcagaggct
     5581 gtgcatcaag ccggaggagg tactggtaac gcgcttttgt cagccagagg gtggaggcag
     5641 catccagctc ctccctggct gccggattga gcgcaggatc cgggtacact ttcatcaaag
     5701 actgaacgat atcctccctg acggttttga tttcaccggc cattttcacg aagttcagaa
     5761 aggcactgtg acggcgaagg tgagtgaagc tatggcgttc ggggtctgct ttcaggaaaa
     5821 agtaaccggg aaacacggcg tgtatatgtc gacggtaacc gcagacttta tccgtgcggc
     5881 ggactttttt cagtatcagg ggtgtccagg gaaggacgtg ttgttcggaa agccaggaaa
     5941 agagatgttc acggtttttg ccggcaggga tgtactgagc cagataccag ttgcgttgat
     6001 gtaattcact gatattcaaa atccggaacg ctccgcgaaa atcgatcccc gcagaggggc
     6061 ttcgatgttg ttgtgaacgg cgaatacata aagcatgtgt cggaaacagc ggctgaccct
     6121 cgttcagccc ggcctgcagt tctgacaggc acatgattta cgcactcttg taagccttgt
     6181 ggacggtttc gaggcgggcg aaaaccgaca acttctgcaa acaggattaa tggctggcaa
     6241 atatattaca taaggatgta tctgtggcaa gagtgaagat aagcagttaa atagatcgtt
     6301 atatttaata aatcagcata ttatgctgat ttgtgtgcgg tgtctggagg attagatcgt
     6361 ttaaaaattt taggcgatct cttctactaa aaatgatgaa aaacacagca taaaagatcg
     6421 tttgtggaat tgtctatttt tttgacatag ttcaaaaaaa cgacgttttt taggtggtta
     6481 atgagaggcc tggtaaatca gagaaatcgt gtcggctttt caaagcggtg gaaaaggggt
     6541 atattgcgga tcgttatgca gtggcttttg ggatcgtcag gatccgggaa gtcagaaaac
     6601 ggcaggatgc gccataaggc attcaggatg tatggcagaa acgacggcag tttgccggtg
     6661 ccggaaggct gaaaaaagtt tcagaagacc ataaaggaaa acccccataa tcttcttatc
     6721 gaacttggcg gaacgtagag aagattatgg gggcctcaca gaatacggga taagtatata
     6781 tgaaaccgta ccagagattc aacccttcgc agtgcattaa cgcacggcat tctgtttccg
     6841 ccataagcga cagcttgtgg cggccctgaa ccagacctca tctactgaaa gcacataccg
     6901 tcaggtaaag aacccgaatc cggtcttcac accccgtgaa gatgccggga cgctgaagtt
     6961 ctgcgaaaaa ctgatggaaa aggcggtggg tttcacttcc cgtttcgatt tcgccgtgta
     7021 tgtggcgtat gcccgttccc gggggctgcg tcggcgcatg ccaccggtac tgcgccgacg
     7081 tgccattgat gcgctgctgc aggggctgtg tttccactat gacccactgg ccaaccgcgt
     7141 ccagtgttcc atcaccacgc tggccattga gtgcggactg gcgacggagt ctgctgccgg
     7201 aaaactctcc atcacccgtg ccacccgggc cctgacgttc ctgtcagagc tggggctaat
     7261 tacctaccag acggaatatg acccgcttat tggctgttat attccgaccg acatcacgtt
     7321 cacaccggca ctgtttgccg ccctcgatat ttccgaagag gcggtggcct ccgcacgccg
     7381 cagccgcgtg gaatgggaaa acaggcagcg caaaaagcag gggctggatg cgctgggtat
     7441 ggatgaactg atagcgaggg cctggcgttt tgtgcgcgag cgtttccgta gttatcaggc
     7501 agagcttaag tcccacggca tgaaacgtgc ccgtgcgcgc cgtgatgcag gcagaacccg
     7561 gcaggacatt gtcaccctgg tgaaacggca gctgacgcgt gaaatcgcgg aagggcgttt
     7621 ccggggaagc ctggaggctg tgaagcgcga aatcgaccgt cgcgtgaagg aacgcatgat
     7681 aatgtcgcgg aacaacaact acacacggct ggccacagcc tcaccctgag ttt
//
LOCUS       DAZFLD010000202         7687 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.203, whole genome shotgun sequence.
ACCESSION   DAZFLD010000202 DAZFLD010000000
VERSION     DAZFLD010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7687
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.203"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(109..732)
                     /locus_tag="ACHKBL_004019"
     CDS             complement(109..732)
                     /locus_tag="ACHKBL_004019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001579788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFO5820816.1"
                     /translation="MQERIDYHIEKYQFRARNESPRLMRQWADVMRECRESGAGARER
                     LRTALLNVDYVTSFELPFRLLLTRTPQMIDTLRREWLLSQKKALFNGRQRGCVYSLQN
                     DLSGVPDCFSYSLATRIRRMDCYGMTTRCFTDIAGQVKAPTARLKLALESGLLVTALD
                     GLFWLGTQRIAADVQRLRQSGMVIVTREVEVSDSLTGTTRLVSAYTL"
     gene            881..1366
                     /locus_tag="ACHKBL_004020"
     CDS             881..1366
                     /locus_tag="ACHKBL_004020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HFO5820817.1"
                     /translation="MIKYIFALTLLLSGSVCADQIQGLVIRVLDGDTLEILQARHPVR
                     IRLVNIDAPEKKQPFGRWSMNQLKSLVVNTPVTVTYTQTDRYGRILGRVYTEDGMEVN
                     RWLVQHGAAWVYPDFNTDYTLPAYQREARTMKRGLWADKKPVPPWLWRKENKNSHNNY
                     K"
     gene            2010..2374
                     /locus_tag="ACHKBL_004021"
                     /pseudo
     CDS             2010..2374
                     /locus_tag="ACHKBL_004021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997724.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2384..2575
                     /locus_tag="ACHKBL_004022"
     CDS             2384..2575
                     /locus_tag="ACHKBL_004022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000264907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5820818.1"
                     /translation="MWILIFWLSSPFNVSSGDARQPTLAVHSQEFRTEKACRDAFTAL
                     KKENVGEFALRGVCVPGGE"
     gene            2609..2932
                     /locus_tag="ACHKBL_004023"
     CDS             2609..2932
                     /locus_tag="ACHKBL_004023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000323308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlgM"
                     /protein_id="HFO5820819.1"
                     /translation="MAYHSPDITLHYPYLRRMSWAQITAAAEDAEIHHDYARALILWQ
                     HAYHAATLTLNKNLAVAKIDFCIKRIRMREQMSGIIRKTGTDEILFRLSKHHHLYEKK
                     KTKEG"
     gene            complement(3075..3170)
                     /gene="dqlB"
                     /locus_tag="ACHKBL_004024"
     CDS             complement(3075..3170)
                     /gene="dqlB"
                     /locus_tag="ACHKBL_004024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinQ-like type I toxin DqlB"
                     /protein_id="HFO5820820.1"
                     /translation="MINLLIIVLRAVVAVANAVIAILELIRELID"
     gene            3669..4931
                     /gene="trbA"
                     /locus_tag="ACHKBL_004025"
     CDS             3669..4931
                     /gene="trbA"
                     /locus_tag="ACHKBL_004025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000121930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TrbA"
                     /protein_id="HFO5820821.1"
                     /translation="MSYQHSPTTEDPMNIWYIVGTICLLFAFLIWLFLPDIVFATCLI
                     LHTMWGMTDWGPFHHFAAPRYNLLANTANNAATITFSQWLDVMSQTVGILWLILLPMT
                     LGFLWMWFQHPAQPRFTRRPLNIHTLPHLFSSLSPAIAPVLADGDSNRLFHGQKRPER
                     RVALTPEVFVEQNNLIRNMQLDVASTRQCFMAQLGQPLTSWKDMAPHEKALFAIFGLQ
                     FFLGDRKAAVALMNNLNLSCRLKSKRDQGRFSTPVYSLARNAFIRVIKTEGAQQWLRQ
                     HRYVRSGLVWLYAHDLRLTPPNWLWLKGVDRTLFYALHRANTTKGFIEGAGVVAVARA
                     ENEACRLGLPCPEPCVEEAIEGLRQDMLRLGLIWDEPQPDRDRRRQIRTRWSLTDDVI
                     PRRHDNDEGSDTGETTETRHPADKEKAQ"
     gene            4928..6046
                     /locus_tag="ACHKBL_004026"
     CDS             4928..6046
                     /locus_tag="ACHKBL_004026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004031782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbC family protein"
                     /protein_id="HFO5820822.1"
                     /translation="MTIRYTATLNNKHLSLTALYPDGGQEVIAILPVTELGALINRNN
                     RLMHLSFSTPFREITETLLVDTVDTEPPGHHIGRMLSAIDSCLSAAYAELPARKTSFM
                     RGLIYGAGLVLALLTAVTAGYILLKPDSDSPLRVPVTESVTETAPLHIDKEIVRSVAP
                     SETKNEIVHPAPQEMQETSATPAITADKNTLVAEARRNLATVLKRNADRGMFTINLST
                     GHERTLYAFLDPACINCRLLEPALKQLAREFSVVIYPVSVIGGEASTNRTAPLLCEPD
                     AQKRATGWHQLYSADGGMAPTGDAPAAPDEKCLTAARAAIDVNNQAFRQFGFGGTPWV
                     LSDTGWHLPSGILKDATTVSLFLKATDTEDNHNEPASR"
     gene            6027..>7687
                     /gene="trbC"
                     /locus_tag="ACHKBL_004027"
     CDS             6027..>7687
                     /gene="trbC"
                     /locus_tag="ACHKBL_004027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001289278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F-type conjugative transfer protein TrbC"
                     /protein_id="HFO5820823.1"
                     /translation="MSQHHVNPDLIHRTAWGNPLWNALHNLNIIGLCLAGSIITALIW
                     PLALPVCLLFTLVTSVIFTLQRWRCPLRMPMTLSLDDPSQDRKVRRSLFSFWPTLFQY
                     EADETSPARGIFYVGYRRINDIGRELWLSMDDLTRHIIFFSTTGGGKTETTFAWLLNP
                     LCWGRGFTFVDGKAQNDTTRTIWYLSRRFGREDDIEVINFMNGGKSRSEIIQSGEKSR
                     PQSNTWNPFAFSTEAFTAETMQSMLPQNVQGGEWQSRAIAMNKALVFGTKFWCVRERK
                     TMSLQMLREHMTLEGMAKLYCRGLDDQWPEEAIAPLRNYLQDVPGFDMSLVRTPSAWT
                     EEPRKQHAYLSGQFSETFTTFAETFGDVFAADAGDIDIRDSIHSDRILIVMIPALDTS
                     AHTTSALGRMFITQQSMILARDLGYRLEGTDAQTLEVKKYKGSFPYICILDEVGAYYT
                     DRIAVEATQVRSLEFSLIMTAQDQERIEGQTSATNTATLMQNAGTKFAGRIVSDDKTA
                     RTVKNAAGEEARARMGSLQRHDGVMGESWVDGNQITIQMESKIDV"
BASE COUNT         1992 a         2162 c         1831 g         1702 t
ORIGIN      
        1 tacttctgat ttatctcgca taaaatatcc tgtctgtaat ctgtcccggt aaacaatggt
       61 aaatttcaga atcagtgcga atacggtaac aaagattcct cctgtgcttt aaagtgtgta
      121 ggcagaaacc agtctggtgg ttccggtcag ggagtctgat acctcaactt cccgggtgac
      181 tataaccata cctgactgac gcagtctctg cacatcagct gcaattcgct gagttcccag
      241 ccagaacagg ccatccagtg ccgttaccag caacccgctt tccagcgcca gtttcagccg
      301 tgccgtcggg gctttaacct gcccggcgat atcagtaaaa caacgcgtcg tcatgccata
      361 gcagtccatc cgccgtattc gcgtcgcaag actgtagctg aaacaatccg gcacgccgga
      421 taagtcattc tgcagactgt atacacatcc ccgctgccgc ccgttgaaca gggctttttt
      481 ctgactgagc agccattccc gacgcagagt gtcaatcatc tgcggtgtcc gggttaacag
      541 cagacgaaac ggcagctcaa aactggtcac ataatcaaca ttcagtaatg ctgtccgcag
      601 tcgctccctg gcacctgccc cgctctcccg gcattcacgc atgacatcag cccactgacg
      661 catcagtcgt ggagactcat tcctggccct gaactgatat ttctcaatat gataatcaat
      721 acgttcctgc atatcgccac cggatatctg ttgtttaccc agacacagtg attagcctac
      781 aggaataaat atcctccgtg aagccaggtg cctcagaaga taaaaacatg tacttttttc
      841 aacgtgtaat attaacacca cctccacagg aaaatataaa atgataaaat atatattcgc
      901 actcacactt ctgctttccg ggtctgtctg tgccgatcaa attcagggac tcgttattcg
      961 tgtgctggac ggagatactc tggagatatt acaggcccgg catcctgtga ggattcgtct
     1021 tgttaatatt gatgcaccgg aaaagaaaca gccttttggg cgctggtcaa tgaatcaact
     1081 taaaagcctt gtggtaaata cgcccgtaac cgtgacatac acacagacag accgctacgg
     1141 gcgtattctg ggacgggtat ataccgaaga cggcatggaa gttaaccgct ggctggtaca
     1201 gcatggtgca gcctgggtat atcctgattt caacacagat tacaccctgc cggcatacca
     1261 gagagaagcc agaacaatga aacggggtct gtgggcagac aaaaaacctg ttccaccatg
     1321 gctgtggcga aaagaaaata aaaatagcca caataattat aaataagaca tctcacagaa
     1381 atatatttcc tgccgtttta tttcccctgc cagtccccaa cgggaaataa cccacagaaa
     1441 ttacacagat gtgaataata aacctgttcg atgtatccgt cacaaaagag ttgatgtttg
     1501 actcagaaga aaatacgcct gaagttaagg aaggcttcag cttcatgatt gttctgacat
     1561 gaacatatat ccctctgtag cgccctgcct ttcataacat ttctattaca gcaacaacga
     1621 aagacaacag caatgtcggc cttttacgtc cacatgaata ttccacttat ccggaattgt
     1681 gacacagccc ctgaatacag gattgattgc cagtgcccac aaatggcaca ttttcagtaa
     1741 gattctgaat gttacattcc cccgtcatgt gtggcacaac tgtcaccggg aaagaaacgg
     1801 aaagcacatg aaaaacatca aaactcacac cggactcctt atccacaaag aacaaacgcg
     1861 gcgcgttcgt cttcatgaaa ctccggccgc ctggtgccac acgcaccggg aatgctacag
     1921 caaaacgacc ggtcgtcgct gcggcagccc tgagagtctc agcaggctga ttctgagcag
     1981 cattcagccc atagaatgag atgacccgta tgaatgatgg cagacagacg cctccgtccc
     2041 ctgaggcatg gaaaatccgg tttctggcga acggtacacc cggcacatgg ttttacacgg
     2101 atgagcagcc gtcacaaaag agtggctgcg ggcacattgt gacgatgtgg aagtgatccc
     2161 cctgtgccgt atgcatgacg gtcccgtttt ccgggaacac catattaatg agctcagcgc
     2221 cgcactggcc agaatgctcc atatgcacca catactgctg gaacgaaccc ggtacagaga
     2281 aaatatccat gatgcagaat gtattcgtga aattcacgaa gctcccctgc aggctatcgg
     2341 catcctggaa aaatacaaac gcacaaaatc ataaggcagt tctatgtgga tactgatttt
     2401 ctggctctca tcccccttta atgtctcctc cggcgatgcc cggcaaccca cactggccgt
     2461 tcacagtcag gagttccgta ctgaaaaagc ctgtcgggat gccttcaccg cgctgaaaaa
     2521 agaaaacgtg ggagaattcg ctctccgggg cgtctgcgtc cccggcggag aataaaccgg
     2581 agatgatgat tagccacagg agtattcaat ggcataccat tccccggata tcacactgca
     2641 ttatccgtat ctgcgccgga tgtcctgggc gcagataacg gcagcagctg aagatgcgga
     2701 aatacatcat gattatgccc gggcattaat attgtggcag catgcatatc atgctgccac
     2761 gctgaccctc aacaaaaacc tggcagttgc taaaatcgac ttctgtatta aacgtatccg
     2821 gatgcgtgaa caaatgagcg ggataatccg gaagactggc acagacgaaa tactcttccg
     2881 tctgagtaag caccatcatt tatatgagaa aaagaaaaca aaagagggat gacaatccca
     2941 tgatatggga aaaacttgcc atgcttactg caggtgcatt tttgatactg gcagtcctgt
     3001 gggggtaata atatcctgtt tattaaaggg cagaaggcgg tgcccctctg cccttaagta
     3061 cgtttccggg attatcagtc aataagttcc cggatcagtt ccaggattgc tatcactgcg
     3121 ttagccacag caacaaccgc cctgagaacg atgattaaca ggttaatcat ccgtttctcc
     3181 gtcaatggag gaaatcactg ttgtttactc ttacattgcc tgtcacaaca gctctttcat
     3241 tgttagatgt aaagtgattt ctctccagcc agagcactga acacagcacc acctgttcga
     3301 tcagccagga cttaaaatct cgcgttcatc gtcccattgg agatcacggc agaagcatca
     3361 ccagacagag ctaacggagc gattatagtc atttttctga ttgcaaaaaa gagaagcgca
     3421 gcctactatg atggtgttag atgtagagtg atttcccttt cggtcagagt caccaactcc
     3481 gccccggaat agcaaaaaaa ccgacctcac cagtcggttt ttttgtgcct gtctctcact
     3541 gccttctgct gttcctgtga aaataccctg gttatttcat ccctgcccgg tcattaccgg
     3601 cgattgttcg cattaattcc actcagcctc atcccgaaat ttatccattt atcgatggag
     3661 gaatactcgt gtcttaccaa cactcaccca caactgaaga cccgatgaat atatggtata
     3721 tcgttggcac catatgcctg ctctttgcgt ttctcatctg gctcttttta cccgacatcg
     3781 tctttgccac ctgcctgatc ctgcacacaa tgtgggggat gactgactgg gggccttttc
     3841 accattttgc cgccccccgt tataacctgc tggccaacac cgccaacaac gctgcaacga
     3901 tcactttcag ccagtggctg gatgtcatgt cacagaccgt ggggatcctc tggctgattc
     3961 tgcttcccat gacgctcggc tttctgtgga tgtggtttca gcatccggcc cagccccgct
     4021 tcacccgcag accactgaat atccacacgc tgccacacct gttttcatca ctgtcaccgg
     4081 cgatcgcccc cgttctggct gacggagaca gcaacagact cttccacgga caaaaacgtc
     4141 ccgagcgccg tgtcgcacta acccccgaag tctttgtgga gcagaacaac cttatccgca
     4201 acatgcagct cgatgttgcg tccacacgcc agtgcttcat ggcgcaactg gggcaaccac
     4261 tgaccagctg gaaagacatg gccccacatg aaaaagcgct ctttgccatc tttggcctgc
     4321 agttcttcct cggtgaccgg aaagcagcgg tagccctgat gaacaacctc aatctctcct
     4381 gcaggctcaa aagtaaacgg gaccaggggc gtttcagcac gccggtgtat tcactggcca
     4441 gaaacgcctt tatccgggtc atcaaaacag aaggcgcaca acagtggctc aggcagcacc
     4501 gttacgttcg cagcggtctg gtctggcttt acgcacatga tttgcgcctg acgcctccga
     4561 actggctgtg gctcaagggc gttgaccgga cgctcttcta tgcccttcac cgggcaaaca
     4621 ccacaaaagg cttcatcgaa ggggccggcg tggtggccgt tgcccgggca gaaaatgaag
     4681 cgtgtcgtct cggcctgccc tgcccggaac cctgtgtgga agaagccatt gaagggctgc
     4741 ggcaggacat gctgagactg gggttgatat gggacgaacc tcagccagac cgcgatcgca
     4801 gacgtcagat aaggacccgc tggtcactca cggatgacgt gataccacgc cgccatgata
     4861 acgatgaagg ctcagatact ggtgaaacca ctgaaacccg gcatcccgcc gacaaggaaa
     4921 aagcacaatg accatccgct acactgcaac actgaacaat aaacacctgt ccctgaccgc
     4981 cctgtaccct gacggtggtc aggaggttat tgccatcctg ccggttacag aactgggcgc
     5041 actgattaac cgtaataaca ggctgatgca cctgtccttc agcacccctt ttcgtgaaat
     5101 aaccgaaaca ctgcttgttg ataccgttga tactgaacct cccggacatc acatcggcag
     5161 aatgctctcc gcgattgaca gctgtctgag cgcggcatat gcagaacttc ctgcccggaa
     5221 aaccagcttc atgagagggc tgatatacgg tgcaggatta gtgctggcac ttctgacagc
     5281 ggtaacagcc ggatatattt tactgaaacc ggacagcgat tcccctctca gggtacctgt
     5341 cacagaatcc gtaacagaaa cagcaccatt acacatcgac aaggaaattg tccggtcagt
     5401 agccccgtca gagacgaaaa atgaaatcgt tcacccggca ccacaggaaa tgcaggagac
     5461 atccgctacc ccggcgataa cagcagacaa aaatactctg gtcgctgaag cccgccgtaa
     5521 tctggccact gtacttaaac gcaacgcgga ccggggtatg ttcaccatta atctctccac
     5581 tggtcatgaa cgcacactct acgccttcct tgaccccgca tgcatcaact gccgtcttct
     5641 ggagcccgcg cttaagcaac tggcccggga attcagcgtg gtgatttatc cggtctccgt
     5701 aatcggcgga gaggccagca caaaccggac tgcgccactg ctgtgtgaac cggacgcaca
     5761 aaaacgggcg accggatggc atcagctgta ttccgcagac ggtggtatgg cacccaccgg
     5821 cgatgcaccg gctgcaccgg atgaaaagtg tctgaccgct gctcgtgctg ccatcgatgt
     5881 caataaccag gctttccgcc agttcggttt tggcggtacc ccatgggtgt tatccgatac
     5941 cggctggcac ctgccgtccg gcatcctgaa agacgccact accgtcagcc tgttcctgaa
     6001 agccacagat accgaggata atcacaatga gccagcatca cgttaatcct gacctcatac
     6061 accgcacggc ctggggaaac ccgctgtgga atgcactgca caacctgaac atcattggtc
     6121 tgtgtctggc cggcagtatc attacggccc tgatatggcc actggccctt cctgtctgtc
     6181 tgctgttcac cctggtcacc tccgtgatat tcacgctgca acgctggcga tgccccctgc
     6241 gtatgcccat gacgctcagc cttgatgacc cctcccagga cagaaaggtc aggcgaagcc
     6301 tcttcagctt ctggcccacc ctgtttcagt atgaagcaga tgaaacatcc cccgcacggg
     6361 ggatttttta tgtcggttac cgccgtatca acgatatcgg tcgtgagctc tggctgagca
     6421 tggatgacct cacccggcat atcatatttt tttccaccac cggtggcggt aaaacagaaa
     6481 ccacctttgc ctggctgctc aaccctctct gctggggaag aggctttacc tttgttgacg
     6541 gaaaggcaca gaacgatacc acgaggacaa tctggtatct ctcccgacgt tttggccggg
     6601 aggatgatat cgaggtcatc aacttcatga acggcggaaa atcccgcagc gaaatcattc
     6661 agagcggcga aaaatcccgc ccacagtcaa acacctggaa cccgttcgca ttcagcacag
     6721 aagccttcac cgccgaaacc atgcagtcca tgcttcccca gaacgttcag gggggagaat
     6781 ggcagtcacg ggcgatagcc atgaacaaag cccttgtctt tggcacaaaa ttctggtgcg
     6841 tccgggaaag aaaaaccatg tccctgcaga tgttacggga gcatatgacg ctggaaggta
     6901 tggcaaaact ctattgccgt gggcttgatg accagtggcc ggaagaagcc atcgcaccac
     6961 tgagaaatta cctgcaggac gttccgggat tcgatatgtc actggtgaga acgccgtcag
     7021 catggactga ggagccccgt aaacagcatg cttatctctc cggtcagttc tcggaaacgt
     7081 tcacaacctt tgccgaaacc ttcggggacg tctttgcggc agatgccggc gacatcgata
     7141 ttcgcgacag tattcacagc gaccgcattc tgattgtgat gatccccgca ctggacacat
     7201 cagcccatac cacgtcggca ctcggtcgta tgttcatcac ccaacagagc atgatcctcg
     7261 cccgtgacct gggctaccgc ctggaaggca cagatgcaca gaccctggaa gtcaaaaaat
     7321 acaaaggcag cttcccctat atctgtatcc ttgacgaagt cggtgcctac tacacagacc
     7381 gcattgctgt ggaggccacc caggtacgtt cactggagtt ttccctgata atgacggcgc
     7441 aggatcagga gcgaattgaa gggcagacct ccgccaccaa caccgccaca ctgatgcaga
     7501 acgccggcac caaattcgcc ggacgcattg tcagtgacga caaaacggca agaacggtga
     7561 aaaatgccgc cggtgaagag gcacgggcca gaatgggcag cctgcagcgc cacgatggtg
     7621 tgatgggtga gtcctgggtg gacggtaacc agatcaccat ccagatggaa agcaaaatcg
     7681 acgtgca
//
LOCUS       DAZFLD010000203         7682 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.204, whole genome shotgun sequence.
ACCESSION   DAZFLD010000203 DAZFLD010000000
VERSION     DAZFLD010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7682
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.204"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(1..342)
                     /locus_tag="ACHKBL_004028"
     CDS             complement(1..342)
                     /locus_tag="ACHKBL_004028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001537072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO5820824.1"
                     /translation="MDEIKTLHWCPREGMQVTEKPSVVTVKFGDGYQQRRPAGLNAQL
                     KTFQVVFRVTTDAEREALSAFLSWHGGYRAFLWKPPKHNRTVRVVCREWSITDNARYS
                     DFSCTIEQVVR"
     gene            complement(335..3577)
                     /locus_tag="ACHKBL_004029"
     CDS             complement(335..3577)
                     /locus_tag="ACHKBL_004029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO5820825.1"
                     /translation="MATLRELIIKISANSQSFQSEIQRASRMGSEYYRTLQNGGRQAA
                     AAAREQRRALAELNSQLTEIRASAVGMTGAFAGAFATGHLISLADEWSSVNARLKQAS
                     QSSDEFSSSQKVLMDISQRTGTAFSDNAALFARSAASMREYGYSADDVLKVTEAISTG
                     LKISGASAAEAGSVITQFSQALAQGVLRGEEFNSVNESGDRIIRALAAGMGVARKDLK
                     AMADDGQLTADKVVPALISQLGILRDEYAAMPETVSDGITKVENAFMAWVGGANEASG
                     ATKTLSGALNGVAGNIDTVATVAGVLVAVGVARYFGNLASGAMSATAGLVTAARNEVA
                     LAEAQLRGTQIATARARAAVYRAQQAVAAARGTEMQIAAEARLAITQERLNRNIAART
                     AAQNALNSTTAVGSRLMSGALGLVGGVPGLVMLGAAAWYTLYQNQEQARESARQYALT
                     IDEIAHKTPSMSLPEASDNEERTREALTEQNRLIDEQASRVKSLQEKIAGYQYVLANP
                     GWTTGNGFMINHLTSVKTVTEELAQATEQFAVEQSRLAQMQEKAQSIQDVLAGLEERR
                     VVLIRQQAAEQNKAYQSLLVMNGQHTEFNRLLGLGNELLQQRQGLVNVPLRLPQATLD
                     DKQQSALNNTERQLALSRLKGEEKERARLGYAADDLGLVGDTYQEARQRYIRNSMEAW
                     RNNEANKPKSRAGKSETEKAEDSFSRLLKQQKEQLALAGKNTELAKLKYQTSQGELKT
                     LTEIQKQELLRNAALIDQKKIREQLRAREEALKNDNADERASNDAELLGYGQGERVRE
                     RMRELQQIRDGYRQKDADLQSQYQTGDISEDFYRQALAQNAQYLSERLKDQEAFYAES
                     DAQRADWQKGLQEGLSNWVDSASDYASQAAQLATDGISGMVNNITEMLNGNKVEWRSW
                     AASILQEISKVLMNAAIVNGIKMAANSMSGAGGFFGSIGNWLGGAVANAKGGVYTSAN
                     LSAYSNSIVDTPTYFAFAKGAGLMGEAGPEAIMPLTRAADGSLGVRAVGSMNGSAGLV
                     YSPVYHIAIQNDGANGQIGPEAAGTLVQLIDQRVQAVMLSMRRDGGMLSG"
     gene            complement(3641..4054)
                     /locus_tag="ACHKBL_004030"
     CDS             complement(3641..4054)
                     /locus_tag="ACHKBL_004030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5820826.1"
                     /translation="MKKFMLLIFSVSFFLTGCATIVGDKTQTVQVNSNPSGADFIIKD
                     ESGKTISSGKTPQNVTLEKSDGSYFGKKSYQITFSKENYQPVTLPIKASANGWYIGGN
                     FVFGGIVGWLLVDPFNGGMYTLSPESLNPGLVPKT"
     gene            complement(4118..4327)
                     /locus_tag="ACHKBL_004031"
     CDS             complement(4118..4327)
                     /locus_tag="ACHKBL_004031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4035 domain-containing protein"
                     /protein_id="HFO5820827.1"
                     /translation="MWMEFDRVSPLGDERGDIRNAQIVKAVFGAQGMNVALKDAMLCW
                     GEDEDKPEADPLAALEDALLFASEN"
     gene            complement(4423..4797)
                     /locus_tag="ACHKBL_004032"
     CDS             complement(4423..4797)
                     /locus_tag="ACHKBL_004032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly chaperone"
                     /protein_id="HFO5820828.1"
                     /translation="MKKDLKTLALARLSGFRHKTVKVPEWGNVSVVLREPSAEAWYLW
                     QDVLNGDGEDDDTLSVVAKTRRNLEADVTLFCDVLCDTDLQRVFTPDDREQVLAVYGP
                     VHARLLRQALELIADAESARKK"
     gene            complement(4812..5528)
                     /locus_tag="ACHKBL_004033"
     CDS             complement(4812..5528)
                     /locus_tag="ACHKBL_004033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major tail subunit"
                     /protein_id="HFO5820829.1"
                     /translation="MSALYERSQLTQVMISSAPATAETMDKAEYLRLDCTIKEVQFTA
                     GQKQDIDVTTLCSTEQENINGLGASSEISMSGNFYLNQAQNALRDAYDNDALYAFKVQ
                     FPSGKGFKFLAEVRQHTWSSGTNGVVAATFSLRLKGKPVSFVVPLAFVKNLDKTLTVN
                     TGALLTMSVSANGGTPPYKYAWKKDGQPVDGQTTDTFSKPGAQSADAGKYTCVVTDSA
                     EKAQSVTSVECTVTVSAAAG"
     gene            complement(5594..5938)
                     /locus_tag="ACHKBL_004034"
     CDS             complement(5594..5938)
                     /locus_tag="ACHKBL_004034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3168 domain-containing protein"
                     /protein_id="HFO5820830.1"
                     /translation="MTEADLYPHLAHLAGGQVYPYVVPLLDGRPSVALPWVVFSLISS
                     VSADVMGGQAESSVAVQIDVYAGTVTQARQIRQDAREAIMLLAPGSVSEMQDYIPENR
                     CYRATLEFQVTV"
     gene            complement(5935..6381)
                     /locus_tag="ACHKBL_004035"
     CDS             complement(5935..6381)
                     /locus_tag="ACHKBL_004035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97-gp10 family putative phage morphogenesis
                     protein"
                     /protein_id="HFO5820831.1"
                     /translation="MIETLLDFSGLEEISRDLQLLSGAENNRVLREATRAGANVLKEE
                     VASRAPVRRGKLRRNVVVLSRRSRDGGMESGVHIRGVNPDTGNSDNTMKADNPRNAFY
                     WRFVEMGTVNMPPHPFVRPAFDVRSEQAAQVAIARMNRAIDEVLRR"
     gene            complement(6378..6728)
                     /locus_tag="ACHKBL_004036"
     CDS             complement(6378..6728)
                     /locus_tag="ACHKBL_004036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFO5820832.1"
                     /translation="MAISAGRLTQMISVLNPVLTRNAAGEMTEEWVSCGKIHADIRGR
                     SSRERMQSGAEMAQAEIRIWVRGQSGREITAASRLHVLSGPWRDRILNVVGLPVPDAT
                     GGRLEILCRLGGEK"
     gene            complement(6738..7064)
                     /locus_tag="ACHKBL_004037"
     CDS             complement(6738..7064)
                     /locus_tag="ACHKBL_004037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFO5820833.1"
                     /translation="MTALLTLEEIKAHLRVDHDADDDMLMDKVRQATAVLLAYIQGSR
                     DKVIREDGELIPGEALTRMKGAAMRLTGMLYRNPDLAEREELLQGELPFSVSVLIYDL
                     RCPTVL"
     gene            complement(7061..>7682)
                     /locus_tag="ACHKBL_004038"
     CDS             complement(7061..>7682)
                     /locus_tag="ACHKBL_004038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001365916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO5820834.1"
                     /translation="AVANIPQPADGKSITPDDVRPMLEQMVKEAVSHIPVPRDGRDYD
                     PDVLQKAVNDAVSALPAPQDGRDATALEILPAIDDQKSFPRGTYATHQGGLWRAYEKT
                     HGMRGWECLVDGVADIDVSMTGERLFSVVVRQSSGQRTEKTFSLPVMLYRGVFRAGET
                     YHPGDTVTWGGSLWHCNSMTGDKPGEAHSSGWTLAAKRGRDAGGGK"
BASE COUNT         1615 a         2519 c         1782 g         1766 t
ORIGIN      
        1 tcatctgact acctgctcta tcgtgcaact gaaatcactg taccgggcgt tatccgtaat
       61 actccactcc cggcacacca ccctgaccgt ccggttatgt ttcgggggct tccacaaaaa
      121 agcccggtaa ccaccatgcc atgacagaaa cgcggacagt gcctcccgct cagcatccgt
      181 tgtcacccga aaaaccacct gaaaggtctt cagttgcgca ttcagtcctg ccggacgacg
      241 ttgctgataa ccgtcgccaa acttcaccgt caccaccgac ggtttctccg tcacctgcat
      301 cccttcccgg ggacaccagt gaagggtctt aatctcatcc actcagcatt cctccgtcac
      361 gacgcatgga taacatcacc gcctgtaccc gctggtcaat cagttgcaca agggtgcccg
      421 ccgcttccgg ccctatctgc ccgttagcgc cgtcattctg aatggcaatg tggtacaccg
      481 gggaatacac cagacccgca ctgccgttca tactgcccac cgcacgcacg ccgagtgaac
      541 catccgctgc ccgggtcagg ggcatgatgg cttcaggtcc ggcttccccc atcagccctg
      601 ccccttttgc aaaggcaaag tacgtgggcg tgtccacaat actgttactg tatgcactca
      661 ggtttgccga ggtataaacg ccgccttttg cattggccac cgcgccaccc agccagttgc
      721 ctatactgcc gaaaaatcct cctgcaccgg acatactgtt tgcggccatc ttaatgccgt
      781 tgacaatggc cgcattcata agaacttttg atatttcctg caggattgat gcagcccagc
      841 tgcgccattc cactttattt ccgttcagca tctccgtgat gttattcacc atccctgaga
      901 taccgtctgt cgcaagctgt gctgcctgtg aagcgtaatc tgatgcgctg tccacccagt
      961 tacttagccc ttcctgcagc cctttctgcc agtcagcacg ctgcgcatcc gattcggcat
     1021 aaaaagcctc ctggtcttta aggcgttcac tcagatactg cgcattctgc gccagcgcct
     1081 gtctgtaaaa atcctcactg atatcccctg tctgatactg agactgcagg tccgcatcct
     1141 tctggcggta gccgtcacga atctgctgca gctcccgcat ccgttcacgg acccgctcac
     1201 cctgcccgta ccccagcagc tcagcgtcat ttgatgccct ttcgtctgca ttatcatttt
     1261 tcagagcctc ctcccgggcc cgtaactgct cccggatttt cttctggtca atcagggcag
     1321 cattgcgcag caactcctgc ttctgtatct ccgtcagggt tttcagctct ccctgcgagg
     1381 tctggtactt cagcttcgcc agttctgtat tcttccctgc cagtgccagt tgttccttct
     1441 gctgcttcag cagacgggaa aaactgtctt ccgctttttc cgtctcagat tttccggccc
     1501 gggatttggg tttattcgcc tcattattgc gccaggcttc catcgaatta cggatgtaac
     1561 gctgcctcgc ctcctgatac gtatccccca ccagaccaag gtcatccgcc gcatacccca
     1621 gccgggcacg ctctttttct tcccctttca gccgggacag ggccagttga cgctctgtgt
     1681 tattcagggc gctctgctgt ttatcatcca gagtggcctg tggcaatcgc agcggtacat
     1741 tcaccagtcc ctgacgctgt tgcagcagtt cattacccag ccccagcagg cggttgaatt
     1801 ccgtatgctg accgttcatg accagcagtg actgatacgc tttattctgc tctgctgcct
     1861 gctgacgaat taacacaaca cggcgctctt ccagtccggc aagcacatcc tgaatggact
     1921 gcgctttttc ctgcatctgt gccagacggg actgctcaac agcaaactgc tctgttgcct
     1981 gagcaagctc ttccgttaca gttttcaccg atgtcagatg gtttatcatg aagccgttac
     2041 cggtcgtcca gcccggatta gccagcacat actgataccc ggcgattttt tcctgcaggg
     2101 atttcacccg gctggcctgc tcatcaatca gccggttctg ctctgtcagc gcctcccgtg
     2161 ttcgctcttc attatctgag gcttcaggca gagacattga tggcgtttta tgcgcgattt
     2221 catcaatcgt cagtgcatac tggcgcgcag actccctggc ctgttcctga ttctggtaca
     2281 gcgtgtacca tgcggcagct cccagcatca ccagtccggg tacgcctcca accagtccca
     2341 gcgcaccgct catcagacgt gagcccaccg ccgttgtact gttcagcgca ttctgggcgg
     2401 cggttctggc agcaatattt ctgttcaggc gttcctgtgt gatcgccaga cgggcctctg
     2461 ccgcaatctg catctcagtc ccgcgggctg ccgccacagc ctgctgagcc cggtacacgg
     2521 ctgcccttgc ccgcgccgtg gcaatctgcg ttccccttaa ctgggcctca gccagtgcca
     2581 cttcattacg tgcagcggtc acaagtcctg ccgtggcaga catcgctccg gaagccagat
     2641 taccaaagta ccgggccacc ccgacagcaa ccagcacacc cgcaactgtt gccacggtat
     2701 caatatttcc ggccacaccg ttcagtgcgc cggagagcgt tttcgtcgct ccgctggcct
     2761 cattcgcgcc gcccacccag gccataaagg cgttttccac cttcgtgatc ccgtcagaca
     2821 ccgtttccgg catggctgca tattcatcac gcaatatccc cagctggctg attaacgcgg
     2881 gaacgacttt atccgccgtc agttgaccat cgtccgccat cgccttcaga tccttacggg
     2941 ccacgcccat gcctgcagcc agtgcgcgaa tgatccggtc cccactttca ttgaccgaat
     3001 taaattcctc gccgcgcaac accccctgtg ccagcgcctg gctgaactgg gtgatcaccg
     3061 aacccgcctc tgccgcactg gcaccggaaa ttttcagccc tgtcgaaatg gcctccgtca
     3121 ccttcagcac atcatcagca ctgtaaccat attcacgcat cgaggcagcc gaacgggcaa
     3181 acagggccgc attatccgaa aatgcggtgc ctgtccgctg gctgatatcc atcagcactt
     3241 tctgtgatga cgaaaattca tccgatgact gcgacgcctg tttcagacgg gcattcacgg
     3301 agctccattc atccgccagc gaaatcaggt gtccggtggc aaaggcacca gcaaacgcac
     3361 cggtcattcc gacagccgaa gcgcggattt ccgtcaactg gctgttcagc tcagccagag
     3421 cccggcgctg ctccctggct gccgcagcag cctgacgtcc gccattctgc agggtccggt
     3481 aatattcact gcccatacgg gacgcccgct ggatctccga ctggaatgac tgtgaatttg
     3541 ccgaaatttt gataatcagt tcacgtaacg tcgccattca cctttctccg ggcgaaaaaa
     3601 aacctgccgc agcaggtttt attcatcagc agattattga ttaagttttg ggtaccagcc
     3661 cgggattcag actttccgga ctcagtgtat acataccgcc attgaatggg tccacaagca
     3721 accagccaac aataccacca aagacaaagt taccaccaat gtaccatcca ttagccgatg
     3781 ccttgatcgg cagcgttacg ggctgataat tctctttact gaatgtgatc tgataactct
     3841 ttttcccgaa gtaactgccg tcagattttt caagtgttac attctgcggt gttttgcccg
     3901 aagaaattgt tttccctgac tcatctttaa taataaaatc cgcaccagac ggattactgt
     3961 ttacctgtac agtctgcgtt ttatctccga caatggttgc acaccccgtc agaaaaaagc
     4021 tgacagagaa aatcaacagc atgaatttct tcataaaatg gctccgttca gttaattttc
     4081 cccgaaaata ctgtaataaa gatctgcaaa gttaaaatta attttccgaa gcaaataaca
     4141 gagcatcctc cagagcggcc aaaggatctg cctccggctt atcctcatcc tcgccccagc
     4201 agagcatggc gtccttcagt gcaacattca tcccctgtgc cccgaaaacc gctttcacga
     4261 tctgtgcatt acggatatcc ccgcgctcat cacccagcgg ggataccctg tcgaactcca
     4321 tccacatcat cgcctcgctc gcactcaggc tgtggcgcag ttcggataag gtgcgcccca
     4381 gacggagcgc aagtcgcatc agaaagcgaa tttccgggcg ggctactttt ttctggccga
     4441 ctctgcatca gcgatcagtt ccagtgcctg acgcagcaag cgggcatgta ccggaccata
     4501 gacggccagc acctgctcac ggtcgtccgg agtgaacacc cgctgcaggt ccgtatcaca
     4561 caggacatcg cagaacagcg tcacatccgc ttccaggtta cggcgggttt tcgccaccac
     4621 cgacagggta tcgtcatcct ctccatcacc attgagcacg tcctgccaca gataccaggc
     4681 ctctgccgaa ggctcccgca gcaccacgct gacattaccc cattccggca ccttcaccgt
     4741 tttatgacga aaccctgaca gtctggccag cgccagcgtt ttcagatcct ttttcatgat
     4801 gacccatccc cttatccggc ggctgcgctc actgtcactg tacattcaac agacgtcaca
     4861 ctctgtgctt tctctgccga atcagtcacc acgcaggtat atttccccgc atcagcggac
     4921 tgcgcacctg gcttactgaa ggtgtctgtc gtctgcccgt caaccggctg accatccttc
     4981 ttccaggcgt atttatacgg cggcgttccc ccgttggcac tgactgacat tgtcagcagc
     5041 gcaccggtat tcacggtaag tgtcttatcc agatttttca caaacgccag cggtaccaca
     5101 aaggacaccg gtttgccttt cagacgcagt gagaacgttg cagccaccac gccgttggta
     5161 ccggatgacc aggtgtgctg gcgcacttcc gccaggaatt taaagccctt accggacgga
     5221 aactgcacct taaacgcata caacgcgtca ttgtcataag catcacgcag ggcgttctgg
     5281 gcctgattca gataaaaatt acccgacatg gaaatctcgg acgacgcccc cagaccattg
     5341 atattctcct gctctgtgga gcagagcgtg gtcacatcaa tatcctgttt ctgaccggcg
     5401 gtgaactgga cttccttgat ggtgcagtcc aggcgcagat attccgcctt atccatagtt
     5461 tcagcagtcg ccggggcaga tgaaatcatc acctgcgtca gctgtgagcg ttcatacaaa
     5521 gcagacattc tgcctcctga taataaaaaa cccgcacgcg gcggggtatg ggttttgtag
     5581 aaaaaagaaa aagtcacacc gtgacctgaa actccagggt tgcacggtaa cagcggtttt
     5641 ccggaatata gtcctgcatt tcactgacgg atcccggggc cagcagcatt atggcttcac
     5701 gggcgtcctg acgtatctga cgcgcctgcg tcacagtccc ggcataaacg tctatctgca
     5761 ccgccactga ggactccgcc tgcccgccca tcacgtccgc agacaccgat gaaatcaggc
     5821 tgaaaaccac ccacggaagc gccaccgacg gcctgccatc cagcaggggg accacatacg
     5881 ggtacacctg cccgccggca agatgcgcca gatgaggata caaatccgcc tccgtcatcg
     5941 tctcagtacc tcatcaatgg cccggttcat ccgagcaatc gccacctgcg ctgcctgttc
     6001 actgcgcaca tcaaacgccg ggcgcacaaa cgggtgcggt ggcatattca cggtccccat
     6061 ttccacaaac cgccagtaga aagcattgcg cgggttatcc gccttcatgg tgttatcgct
     6121 gttaccggtg tccggattaa caccacggat atggacaccg gattccatcc cgccatcgcg
     6181 ggagcgccgg gaaaggacca ccacattgcg gcgcagtttt cccctgcgta ccggtgcccg
     6241 tgacgccact tcttctttca gcacattcgc acccgcacgg gttgcctcac gcagcacccg
     6301 gttattttcc gcaccactca gaagctgcaa atcgcggctg atctcctcca gccccgaaaa
     6361 atccagcagg gtttcgatca tttttcccct cccagccgac agagaatttc cagacgcccg
     6421 ccggtcgcat ccggcacggg cagcccgaca acgttcagga tccggtcacg ccatggacca
     6481 ctcagcacat gaagtcgtga cgctgccgtg atttcccgac cggactgacc gcgcacccag
     6541 atgcggattt ccgcctgcgc catttccgca ccggactgca tccgctcccg gctgctcctg
     6601 ccacggatat ccgcatgaat tttcccgcat gacacccatt cttccgtcat ttctccggca
     6661 gcattacggg ttaacaccgg gttcagaaca cttatcatct gtgtcagacg acctgcagat
     6721 attgccattc cccctcctca taacaccgtc ggacaacgca aatcgtaaat cagcacggaa
     6781 acagaaaacg gcagctcccc ctgaagcagt tcttcccgct ccgcaagatc cggattccgg
     6841 tacagcatcc cggtcagtcg catggcagcc cccttcatcc gggttaatgc ctcgcccggg
     6901 atcagttcac cgtcctcacg aatcacctta tcccggctgc cctgaatgta ggccagcagc
     6961 acggcggtag cctgacgaac cttgtccatc agcatgtcat catccgcgtc atggtcgaca
     7021 cgcagatgtg ccttgatctc ttccagtgtc agtaatgccg tcattttccg cctccagcat
     7081 cccgcccacg ttttgcagcc agggtccagc ctgatgaatg agcttctccg ggtttatcac
     7141 cggtcatact gttgcagtgc cacagcgagc ccccccacgt caccgtatcg ccggggtggt
     7201 aggtttcacc ggctctgaac acaccgcggt agagcatcac cggcagggaa aatgtttttt
     7261 ccgtacgctg gccactgctc tgccggacca ccacagagaa caaccgttca cccgtcatgc
     7321 tgacgtcaat atccgccacc ccgtcaacca ggcattccca tccccgcatc ccgtgcgttt
     7381 tttcatacgc ccgccagagt ccgccctggt gtgtggcata cgtgccccgg ggaaaggatt
     7441 tttgatcgtc aatggcaggg agtatttcca gtgccgtggc atcacgcccg tcctgcggag
     7501 ccggcagggc actcaccgca tcattcaccg ccttctgcag aacatccgga tcatagtcac
     7561 gaccatcacg cggaacagga atatggctta ccgcctcctt caccatctgc tcaagcatcg
     7621 gacgcacatc atccggggtg atacttttac cgtctgccgg ctgcggaata tttgcgaccg
     7681 ca
//
LOCUS       DAZFLD010000204         7451 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.205, whole genome shotgun sequence.
ACCESSION   DAZFLD010000204 DAZFLD010000000
VERSION     DAZFLD010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7451
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.205"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            171..4421
                     /gene="fdeC"
                     /locus_tag="ACHKBL_004039"
     CDS             171..4421
                     /gene="fdeC"
                     /locus_tag="ACHKBL_004039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000092542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter adhesin FdeC"
                     /protein_id="HFO5820835.1"
                     /translation="MSRYKTDNKQPRFRYSVLARCVAWANISVQVLFPLAVTFTPVMA
                     ARAQHAVQPRLSMENTTVTADNNVEKNVASLAANAGTFLSSQPDSDATRNFITGMATA
                     KANQEIQEWLGKYGTARVKLNVDKNFSLKDSSLEMLYPIYDTPTNMLFTQGAIHRTDD
                     RTQSNIGFGWRHFSENDWMAGVNTFIDHDLSRSHTRIGVGAEYWRDYLKLSANGYIRA
                     SGWKKSPDVEDYQERPANGWDIRAEGYLPAWPQLGASLMYEQYYGDEVGLFGKDKRQK
                     DPHAITAEVNYTPVPLLTLSAGHKQGKSGENDTRFGLEVNYRIGEPLEKQLDTDSIRE
                     RRMLAGSRYDLVERNNNIVLEYRKSEVIRIALPERIEGKGGQTVSLGLVVSKATHGLK
                     NVQWEAPSLLAAGGKITGQGNQWQVTLPAYQAGKDNYYAISAIAYDNKGNASKRVQTE
                     VVISGAGMSADRTALTLDGQSRIQMLANGNEQKPLVLSLRDAEGQPVTGMKDQIKTEL
                     TFKPAGNIVTRTLKATKSQAQPTLGEFTETEAGVYQSVFTTGTQSGEATITVSVDDMS
                     KTVTAELRATMMDVANSTLSANEPSGDVVADGQQAYTLTLTAVDSEGNPVTGEASRLR
                     LVPQDTNGITVGAISEIKPGVYSATVSSTRAGNVVVRAFSEQYQLGTLQQTLKFVAGP
                     LDAAHSSITLNPDKPVVGGTVTAIWTAKDANDNPVTGLNPDAPSLSGAAAAGSTASGW
                     TDNGDGTWTAQISLGTTAGELDVMPKLNGQDAAANAAKVTVVADALSSNQSKVSVAED
                     HVKAGESTTVTLVAKDAHGNAISGLSLSASLTGTASEGATVSSWTEKGDGSYVATLTT
                     GGKTGELRVMPLFNGQPAATEAAQLTVIAGEMSSANSTLVADNKTPTVKTTTELTFTV
                     KDAYGNPVTGLKPDAPVFSGAASTGSERPSAGNWTEKGNGVYVSTLTLGSAAGQLSVM
                     PRVNGQNAVAQPLVLNVAGDASKAEIRDMTVKVNNQLANGQSANQITLTVVDSYGNPL
                     QGQEVTLTLPQGVTSKTGNTVTTNAAGKVDIELMSTVAGELEIEALVKNSQKTVKVKF
                     KADFSTGQASLEVDAAAQKVANGKDAFTLTATVKDQYGNLLPGAVVVFNLPRGVKPLA
                     DGNIMVNADKEGKAELKVVSVTAGTYEITASAGNDQPSNAQSVTFVADKTTATISSIE
                     VIGNRAVADGKTKQTYKVTVTDANNNLLKDSEVTLTASPAKLVLDPNGTATTNEQGQA
                     IFTATTTVAATYTLTAQVSQNNGQVSTKTAESKFVADDKNAVLAASPERVDSLVADGK
                     TTATLTVTLMSGVNPVGGTMWVDIEAPEGVTEADYQFLPSKNDHFASGKITRTFSTNK
                     PGTYTFTFNSLTYGGYEMKPVTVTINAVPADTEGAEEK"
     gene            complement(4546..5403)
                     /locus_tag="ACHKBL_004040"
     CDS             complement(4546..5403)
                     /locus_tag="ACHKBL_004040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO5820836.1"
                     /translation="MIRQKILQQLLEWIECNLEHPISIEDIAQKSGYSRRNIQLLFRN
                     FMHVPLGEYIRKRRLCRAAILVRLSAKSMLDIALSLHFDSQQSFSREFKKLFGCSPRE
                     YRHRDYWDLANIFPSFLIRQQQKTECRLVNFPETPIFGNSFKYDIEVSNKSPDEEVKL
                     RRHHLARCMKSFKTDIYFVSTFEPSTKSVDLLTVETFAGTVCKQTDSEMSKEWTINRG
                     LYASFRYEGEWEHYPEWARNLYLMELPARGLARMNGSDIERFYYNENFVEIDSNNIVC
                     EIFIPVRPV"
     gene            5652..6521
                     /locus_tag="ACHKBL_004041"
     CDS             5652..6521
                     /locus_tag="ACHKBL_004041"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308365.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFO5820837.1"
                     /translation="MEFSVLSNNLKMPMMGFGVFQVTDKNVCKQSVLNAIRTGYRLID
                     TAAVYGNEDAVGEAVREAISEGLCTREELFITSKLWVQDMLNQDIAAAGIEASLKKSG
                     LEYFDLYLLHQAMRDYFSAWRALEDAYEEGKLKAIGVSNFYPHVLANFCETVRVKPMV
                     NQVELHPYFAQPEALATMKYYNVQPEAWAPLGGGRHKPFENNLLQSIADAHQKSISQV
                     ILRWNIQRGVVVIPKSTHQQRIEENFAIWDFSLTEKEMAQISSLDLGYVGESVKHFNP
                     EFVRGCLAVKIHD"
     gene            complement(6681..7274)
                     /gene="rclC"
                     /locus_tag="ACHKBL_004042"
     CDS             complement(6681..7274)
                     /gene="rclC"
                     /locus_tag="ACHKBL_004042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine species resistance protein
                     RclC"
                     /protein_id="HFO5820838.1"
                     /translation="MERYLHLLSRGDKIGLTLIRLSIAIIFMWIGLLKFVPYEADSIT
                     PFVANSPLMSFFYEHPEDYKQHLTHEGEYKPEARAWQTANNTYGFSNGLGVVEVIIAL
                     LVLANPVNRWLGLLGGLMAFTTPLVTLSFLITTPEAWVPALGDAHHGFPYLSGAGRLV
                     LKDTLMLAGAVMIMADSARDILKQRSNESSSTLKTEY"
     gene            complement(7286..>7451)
                     /gene="rclB"
                     /locus_tag="ACHKBL_004043"
     CDS             complement(7286..>7451)
                     /gene="rclB"
                     /locus_tag="ACHKBL_004043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="reactive chlorine resistance periplasmic protein
                     RclB"
                     /protein_id="HFO5820839.1"
                     /translation="KIILKHISVSSVSASPTVLENAIADMARKYNASSWKVTSMRIDN
                     NSTATAVLYK"
BASE COUNT         2029 a         1651 c         1924 g         1847 t
ORIGIN      
        1 tgagtggcta cagacactgg atatattagg tggcggtgta ctttcttaca taaaggtatt
       61 tccttttctg tggaaaagga aaccgggaaa tccccggttt ttctgacaag cagacgccat
      121 tatttgtgtc tgcctatgtt cgttaattcg ttcatcagga aattatctca atgtcacgtt
      181 ataaaacaga caataaacag ccacgatttc gttattcagt tctggcccgc tgcgtggcgt
      241 gggcaaatat ctctgttcag gttctttttc cactcgcagt cacctttacg ccagtaatgg
      301 cagcacgtgc gcagcatgcg gttcagccac ggttgagcat ggaaaatact acggtaactg
      361 ctgataataa cgtggagaaa aatgtcgcgt cgcttgccgc taatgccggg acatttttaa
      421 gcagtcagcc agatagcgat gcgacacgta actttattac cggaatggcc accgctaaag
      481 ctaaccagga aattcaggag tggctcggga aatacggtac tgcgcgcgtc aaactgaatg
      541 tcgataaaaa tttctcgctg aaggactctt cgctggaaat gctttatccg atttatgata
      601 caccgacaaa tatgttgttc actcaggggg caatacatcg taccgacgat cgtactcagt
      661 caaatattgg ttttggctgg cgtcattttt cagaaaatga ctggatggcg ggggtgaata
      721 cttttatcga tcatgattta tcccgtagtc atacccgcat tggtgttggt gcggaatact
      781 ggcgtgatta tttgaaactg agcgccaatg gttatatccg ggcttctggc tggaaaaaat
      841 cgccggatgt tgaggattat caggaacgcc cggcgaatgg ctgggatatt cgtgctgagg
      901 gctatttacc tgcctggccg cagcttggcg caagcctgat gtatgaacag tattatggcg
      961 atgaagtcgg gctgtttggt aaagataaac gccagaaaga tccacatgcg attaccgctg
     1021 aagtgaatta cacgccagtg cctcttctga ccctgagtgc cgggcataag cagggcaaga
     1081 gtggtgagaa tgacactcgc tttggcctgg aagttaatta tcggattggc gaacctctgg
     1141 aaaaacaact cgatacagac agcattcgcg agcgtcgaat gctggcaggc agccgctatg
     1201 acctggttga gcgtaataac aatatcgttc ttgagtatcg caaatctgaa gtgatccgta
     1261 ttgctctgcc tgagcgtatt gaaggcaagg gcggccagac ggtttccctg gggcttgtgg
     1321 tcagcaaagc aactcacggt ctgaaaaatg tgcaatggga agcgccgtct ttgctggccg
     1381 caggcggaaa aattacgggg cagggcaatc agtggcaagt gacgctcccg gcttatcagg
     1441 caggcaaaga caattattat gcgatttcag cgattgccta cgataacaaa ggcaatgcct
     1501 cgaaacgtgt gcagacagaa gtggttatta gcggagctgg tatgagcgcc gatcgtacgg
     1561 cgttaacgct tgacggtcag agccgtattc aaatgcttgc taacggtaat gagcaaaagc
     1621 cgttggtgct gtctctgcgc gacgccgagg gtcagccagt cacgggcatg aaagatcaga
     1681 tcaagactga actaaccttc aaaccggctg gaaatattgt gactcgtacc ctgaaggcca
     1741 ctaaatcaca ggcacagcca acactgggtg agttcaccga aactgaagca ggggtgtatc
     1801 agtctgtctt tactaccgga acgcagtcag gtgaggcaac gattactgtt agcgttgatg
     1861 acatgagcaa aactgtcact gcagaactgc gggccacgat gatggatgtg gcaaactcca
     1921 ccctgagcgc taacgagccg tcaggtgatg tggttgctga tggtcagcaa gcctacacgc
     1981 tgacactgac agcggtggac tccgagggta atccggtgac gggagaagcc agccgcctgc
     2041 gacttgttcc gcaagacact aatggtataa ccgttggtgc catttcggaa ataaaaccag
     2101 gggtttacag cgccacggtt tcttcgaccc gtgccggaaa cgttgttgtg cgtgccttca
     2161 gcgagcagta tcagctgggc acattacaac aaacgctgaa gtttgttgcc gggccgcttg
     2221 atgcagcaca ttcgtccatc accctgaatc ctgataaacc ggtggttggc ggtacagtta
     2281 cggcaatctg gacggcaaaa gatgctaatg acaaccctgt aactggcctc aatccggatg
     2341 caccgtcatt atcgggcgca gctgctgctg gttctacggc atcaggctgg acggataatg
     2401 gcgacgggac ctggactgcg cagatttctc tcggcactac ggcgggtgaa ttagacgtta
     2461 tgccgaagct caatgggcag gacgcggcag caaatgcggc aaaagtaacc gtggtggctg
     2521 atgcattatc ttcaaaccag tcgaaagtct ctgtcgcaga agatcacgta aaagccggtg
     2581 aaagcacaac cgtaacgctg gtggcgaaag atgcgcatgg caacgctatc agtggtcttt
     2641 cgttgtcggc aagtttgacg gggaccgcct ctgaaggggc gaccgtttcc agttggaccg
     2701 aaaaaggtga cggttcctat gttgctacgt taactacagg cggaaagacg ggcgagcttc
     2761 gtgtcatgcc gctcttcaac ggccagcctg cagccaccga agccgcgcag ctgactgtta
     2821 ttgccggaga gatgtcatca gcgaactcta cgcttgttgc ggacaataaa actccaacgg
     2881 ttaaaacgac gacggaactc accttcaccg tgaaggatgc gtacgggaat ccggtcaccg
     2941 ggctgaagcc agatgcacca gtgtttagtg gtgccgccag cacggggagt gagcgtcctt
     3001 cagcaggaaa ctggacagag aaaggtaatg gggtctacgt gtcgacctta acgctgggat
     3061 ctgccgcggg tcagttgtct gtgatgccgc gagtgaacgg ccaaaatgcc gttgctcagc
     3121 cactggtgct gaatgttgca ggtgacgcat ctaaggctga gattcgtgat atgacagtga
     3181 aggttaataa ccaactggct aatggacagt ctgctaacca gataaccctg accgtcgtgg
     3241 acagctatgg taacccgttg caggggcaag aagttacgct gactttaccg cagggtgtga
     3301 ctagcaagac ggggaataca gtaacaacca atgcggcagg taaagtggac attgagctta
     3361 tgtcaacggt tgcaggggaa cttgagatcg aggccttggt gaaaaactct cagaagacgg
     3421 tcaaggtgaa attcaaggcg gatttcagta ccggtcaggc gagcctggag gtagacgccg
     3481 ctgctcaaaa agtggcaaac ggcaaagatg cctttacgct gacggcaacg gttaaggatc
     3541 aatacggcaa ccttcttcct ggcgctgtgg tcgtctttaa tctgcctcgg ggcgtcaaac
     3601 cgcttgcaga cggtaatatc atggtgaacg ccgacaagga gggtaaagcg gaactgaaag
     3661 tggtttccgt gactgccgga acctatgaga tcacggcgtc agcaggaaat gaccagcctt
     3721 cgaatgcgca gtctgtaacg tttgtggctg ataagactac ggcgaccatc tccagtattg
     3781 aggtgattgg caaccgtgca gtggcggacg gcaaaaccaa acagacgtat aaagttacgg
     3841 tgactgatgc caataacaac ctgctgaaag atagcgaagt gacgctgact gccagcccgg
     3901 cgaagttagt tctggatccc aatgggacgg cgacaacgaa tgagcaaggg caggctattt
     3961 tcaccgccac cacaactgtt gcggcgacat atacactcac ggcgcaagtg agtcagaaca
     4021 acggtcaggt atcaacgaaa accgccgaat ctaaattcgt cgcggatgat aaaaacgcgg
     4081 tgctcgctgc atctccagag cgtgtagatt ctctggtggc ggacgggaag actactgcaa
     4141 cactgacggt tactctgatg tcgggtgtca accccgtagg aggaaccatg tgggtcgaca
     4201 ttgaggctcc ggaaggggtg acagaggcgg attatcagtt cctgccgtcg aaaaatgacc
     4261 atttcgcgag cgggaaaatc acgcgtacat ttagtaccaa caagccaggt acatacacat
     4321 tcacattcaa ctctttgaca tatggagggt atgaaatgaa accagtgact gtgacaatta
     4381 acgccgttcc tgcagatact gaaggcgctg aggagaaata aaaacgacga tgtaataaat
     4441 aataatggcg ttgacatgcg cagcgccatt atttactgaa tacctaccct ggcaccgatt
     4501 agacggtggc agggtagggt aaatcttatg tgatggagtt acaggctaaa ccgggcgaac
     4561 ggggataaaa atttcgcaaa caatgttatt gctatcaatt tcgacgaagt tttcattgta
     4621 ataaaatcgc tcaatatcac tgccgttcat tctggctaac cccctggcag gtaattccat
     4681 cagatagagg tttcgcgccc attcagggta gtgctcccac tcaccttcat agcggaaaga
     4741 ggcatacagt cctcggttaa ttgtccactc tttcgacatc tctgaatcgg tttgtttgca
     4801 taccgtaccg gcaaaagttt caactgtgag caaatcgacc gattttgttg atggttcgaa
     4861 cgtggagacg aaatagatat ccgtcttaaa actcttcata catctggcta aatgatgacg
     4921 tcgcagtttg acttcttcat ccggtgattt attcgacact tcaatatcat atttaaatga
     4981 attaccaaaa ataggtgtct caggaaagtt gactaatcta cactccgtct tttgctgttg
     5041 acgtattaaa aaagaaggga agatatttgc gagatcccaa taatcacggt ggcggtattc
     5101 acggggagag cagccgaata actttttaaa ttcgcggctg aatgactgct gtgagtcaaa
     5161 atgcaaagag agtgcaatat caagcataga tttcgcggag agccggacaa gaatggcggc
     5221 acgacaaagc cttcgtttgc gaatgtattc tcccaaaggc acatgcatga aatttcggaa
     5281 cagaagctgg atgttgcgtc tgctgtagcc agatttctgt gcgatatctt cgattgaaat
     5341 agggtgttcg agattgcact caatccactc caggagctgc tgtagaatct tctgcctgat
     5401 catcacattc tccgttaatt ctttgtcata tcgcacattt tcgttatgcc ctgggcccta
     5461 agtgtatcaa aaacagcaag ggcgaaggca aaagcactgg tagcgtctgc ttcgttgtcc
     5521 ccgaaaatag agaggtttca gtcctacatt attaatgaat tttttgcata agtgatatca
     5581 aaatccacat actaatttga ggttacgttt taacgtagac tcattgttca tgcctaatgg
     5641 agggactgac agtggaattt tccgtattaa gtaacaatct gaaaatgccg atgatgggat
     5701 ttggtgtttt tcaggttacc gataaaaacg tgtgcaaaca gtcagtgttg aacgctatcc
     5761 gcacgggtta tcgactcatc gacactgcag cggtatacgg taatgaagat gccgtcggtg
     5821 aagccgttcg tgaagctata tctgaaggtt tatgtacccg agaagagtta tttataacgt
     5881 ctaaattgtg ggtgcaggat atgttgaatc aggatattgc agcagcaggt attgaagcat
     5941 cattaaaaaa atcgggacta gaatacttcg atctctattt attgcaccag gccatgcgcg
     6001 attattttag cgcgtggcgt gcactcgaag atgcctatga agagggcaaa ttaaaagcga
     6061 ttggggtttc caatttctac cctcatgttc tggcgaactt ttgtgaaacg gtaagagtta
     6121 aaccgatggt caaccaggtc gagttgcatc cctattttgc ccaaccagag gcgctggcaa
     6181 ccatgaagta ttataacgtg cagcctgaag catgggctcc gttaggtggt ggacgacata
     6241 aaccctttga aaataatctg cttcagagta ttgcagatgc ccatcaaaaa tcgatttctc
     6301 aggtcattct gcgttggaat attcaacggg gagtggtcgt tattccgaaa tcgacacatc
     6361 aacagcgtat cgaagaaaat tttgctatct gggatttctc actgacagag aaagaaatgg
     6421 cgcaaattag ttcgcttgat ttgggttatg ttggggaatc ggtaaaacat tttaatcctg
     6481 agtttgttcg tggttgtctt gctgtaaaaa tacatgattg atattaatca cttattttac
     6541 ctgagacgac aagaatcttt taacagggga gtgatattga tcttcactct gtcatatctc
     6601 cggtaatatg gcgtcaggct tcatcatcag aaagggggcc tgacgcctga aaaagtgaac
     6661 aacagacagt gttcggatta tcaatattca gtttttaacg ttgaactgga ttcattactg
     6721 cgttgtttaa gaatatcccg cgccgaatct gccattatca tgacagcacc tgccagcatc
     6781 agagtatctt tcaataccag acgaccagca ccggataaat aagggaaacc atgatgagcg
     6841 tcacccaatg cgggtaccca tgcctccggg gtggtgatta aaaatgagag tgttaccagc
     6901 ggcgtggtaa atgccatcag cccacccaat aaacctaacc agcgattgac aggattagcc
     6961 aaaaccagca gagcaataat cacctccacg acgccaagac cgttggaaaa accataggta
     7021 ttattagccg tttgccatgc tcttgcttct ggtttgtatt cgccttcgtg agtcagatgc
     7081 tgtttatagt cttccgggtg ttcataaaag aacgacatta gtggactgtt tgcgacgaat
     7141 ggtgtaatgc tgtctgcctc gtaagggaca aactttaata acccaatcca cataaaaata
     7201 atcgcaatac tcagacgaat caatgtcagg ccaattttat ctccccgact taacaggtgc
     7261 aggtatcttt ccattgtaaa catccttatt tatacaatac tgctgttgcg gttgaattat
     7321 tatcaattcg catcgatgta actttccagg atgaagcatt atattttctg gccatatcag
     7381 caatggcatt ctcaagaact gtcggtgatg ctgatactga tgagacgctg atatgtttca
     7441 gaattatttt a
//
LOCUS       DAZFLD010000205         7316 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.206, whole genome shotgun sequence.
ACCESSION   DAZFLD010000205 DAZFLD010000000
VERSION     DAZFLD010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7316
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.206"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            1..220
                     /locus_tag="ACHKBL_004044"
                     /pseudo
     CDS             1..220
                     /locus_tag="ACHKBL_004044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="ISAs1 family transposase"
     gene            complement(309..1445)
                     /gene="yibH"
                     /locus_tag="ACHKBL_004045"
     CDS             complement(309..1445)
                     /gene="yibH"
                     /locus_tag="ACHKBL_004045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFO5820840.1"
                     /translation="MDLLIVLTYVALAWAVFKIFRIPVNQWTLATAALGGVFLVSGLI
                     LLMNYNHPYTFTAQKAVIAIPITPQVTGIVTEVTDKNNQLIQKGEVLFKLDPVRYQAR
                     VDRLQADLMTATHNIKTLRAQLTEAQANTTQVSAERDRLFKNYQRYLKGSQAAVNPFS
                     ERDIDDARQNFLAQDALVKGSVAEQAQIQSQLDSMVNGEQSQIVSLRAQLTEAKYNLE
                     QTVIRAPSNGYVTQVLIRPGTYAAALPLRPVMVFIPEQKRQIVAQFRQNSLLRLKPGD
                     DAEVVFNALPGQVFHGKLTSILPVVPGGSYQAQGVLQSLTVVPGTDGVLGTIELDPND
                     DIDALPDGIYAQVAVYSDHFSHVSVMRKVLLRMTSWMHYLYLDH"
     gene            complement(1448..1810)
                     /gene="yibI"
                     /locus_tag="ACHKBL_004046"
     CDS             complement(1448..1810)
                     /gene="yibI"
                     /locus_tag="ACHKBL_004046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HFO5820841.1"
                     /translation="MFLNYFALGVLIFVFLVIFYGIIAIHDIPYLIAKKRNHPHADAI
                     HTAGWVSLFTLHVIWPFLWIWATLYQPERGWGMQSHVASQEKATDPEIAALSDRISRL
                     EHQLAAEKKTDYSTFPEI"
     gene            2347..4260
                     /gene="mtlA"
                     /locus_tag="ACHKBL_004047"
     CDS             2347..4260
                     /gene="mtlA"
                     /locus_tag="ACHKBL_004047"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HFO5820842.1"
                     /translation="MSSDIKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLP
                     NETLAKLVGPMITYLLPLLIGYTGGKLVGGERGGVVGAITTMGVIVGADMPMFLGSMI
                     AGPLGGWCIKHFDRWVDGKIKSGFEMLVNNFSAGIIGMILAILAFLGIGPIVEALSKM
                     LAAGVNFMVVHDMLPLASIFVEPAKILFLNNAINHGIFSPLGIQQSHELGKSIFFLIE
                     ANPGPGMGVLLAYMFFGRGSAKQSAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILG
                     GMTGVFTLTILGGGLVSPASPGSILAVLAMTPKGAYFANIAGVCAAMAVSFVVSAILL
                     KTSKVKEEDDIEAATRRMQDMKAESKGASPLSAGDVTNDLSHVRKIIVACDAGMGSSA
                     MGAGVLRKKIQDAGLSQISVTNSAINNLPPDVDLVITHRDLTERAMRQVPQAQHISLT
                     NFLDSGLYTSLTERLVAAQRHTENEVKVKDSLKDSFDDSSANLFKLGAENIFLGRKAA
                     TKEEAIRFAGEQLVKGGYVEPEYVQAMLDREKLTPTYLGESIAVPHGTVEAKDRVLKT
                     GVVFCQYPEGVRFGEEEDDIARLVIGIAARNNEHIQVITSLTNALDDESVIERLAHTT
                     SVDEVLELLAGRK"
     gene            4625..5773
                     /gene="mtlD"
                     /locus_tag="ACHKBL_004048"
     CDS             4625..5773
                     /gene="mtlD"
                     /locus_tag="ACHKBL_004048"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008926 - mannitol-1-phosphate
                     5-dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0019594 - mannitol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFO5820843.1"
                     /translation="MKALHFGAGNIGRGFIGKLLADAGIQLTFADVNQVVLDALNARH
                     SYQVHVVGETEQVDTVSGVNAVSSIGDDVVDLIAQVDLVTTAVGPVVLERIAPAIAKG
                     LVKRKEQGNESPLNIIACENMVRGTTQLKGHVMNALPEDAKAWVEEHVGFVDSAVDRI
                     VPPSASATNDPLEVTVETFSEWIVDKTQFKGALPNIPGMELTDNLMAFVERKLFTLNT
                     GHAITAYLGKLAGHQTIRDAILDEKIRAVVKGAMEESGAVLIKRYGFDADKHAAYIRK
                     ILGRFENPYLKDDVERVGRQPLRKLSAGDRLIKPLLGTLEYGLPHKNLIEGIAAAMHF
                     RSEDDPQAQELAALIADKGPQAALAQISGLDANSEVVSEAVTAYKAMQ"
     gene            5773..6360
                     /gene="mtlR"
                     /locus_tag="ACHKBL_004049"
     CDS             5773..6360
                     /gene="mtlR"
                     /locus_tag="ACHKBL_004049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol operon repressor MtlR"
                     /protein_id="HFO5820844.1"
                     /translation="MVDQAQDTLRPNNRLSDMQATMEQTQAFENRVLERLNAGKTVRS
                     FLITAVELLTEAVNLLVLQVFRKDDYAVKYAVEPLLDGDGPLGDLSVRLKLIYGLGVI
                     NRQEYEDAELLMALREELNHDGNEYAFTDDEILGPFGELHCVAALPPPPQFEPADSSL
                     YAMQIQRYQQAVRSTMVLSLTELISKISLKKAFQK"
     gene            complement(6371..6580)
                     /gene="yibT"
                     /locus_tag="ACHKBL_004050"
     CDS             complement(6371..6580)
                     /gene="yibT"
                     /locus_tag="ACHKBL_004050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YibT"
                     /protein_id="HFO5820845.1"
                     /translation="MGKLGENVPLLIDKAVDFMASSQAFREYLKKLPPRNAIPSGIPD
                     ESVPLYLQRLEYYRQLYRPKQVEEK"
     gene            6864..7226
                     /gene="yibL"
                     /locus_tag="ACHKBL_004051"
     CDS             6864..7226
                     /gene="yibL"
                     /locus_tag="ACHKBL_004051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibL family ribosome-associated protein"
                     /protein_id="HFO5820846.1"
                     /translation="MKEVEKNEIKRLSDRLDAIRHQQADLSLVEAADKYAELEKEKAT
                     LEAEIARLREVHSQKLSKEAQKLMKMPFQRAITKKEQADMGKLKKSVRGLVVVHPMTA
                     LGREMGLQEMTGFSKTAF"
BASE COUNT         1859 a         1839 c         1842 g         1776 t
ORIGIN      
        1 atatttttaa atgtgactgt catccttcag ataataaagc catcatcgca atagatggaa
       61 aagcactcct gctctttttt gattagagcc gtggcagagg aacaactcat atcatcattg
      121 cgatctcgac aatgtatagt ctgatccttc ggcagatcaa gacggataaa aaatccaatg
      181 agttcacact catcccgaac ttcttaacat aatggatatt aacgaaaaat catcacagcc
      241 gatgaaataa tatgcaaaaa taattagtcc ttacaaggag caaagttcca taatctcccc
      301 cctccccctc aatgatccaa ataaagataa tgcatccaac tggtcattct cagcagcact
      361 ttccgcatca ccgaaacatg gctgaaatgg tcggagtaaa ctgccacttg ggcgtagatg
      421 ccgtcgggta aggcatcgat atcatcgtta gggtccagtt caatggttcc cagcacaccg
      481 tccgtgccag gcacgaccgt taatgattgc aataccccct gcgcctgata agaaccgcct
      541 ggcacgacag gtaaaatact agtcagtttg ccgtgaaaca cctgcccagg tagcgcgtta
      601 aacaccactt ccgcatcatc gccgggtttc agacgtaaca gcgagttttg ccgaaattgg
      661 gcgacaattt gccgtttttg ctcggggatg aaaaccatca ccggacgcaa cggcaaggca
      721 gctgcgtatg tacctgggcg gatcagtacc tgagtaacgt agccattgct tggtgcgcga
      781 atgacagtct gctcaaggtt atattttgct tcagtaagtt gcgctcttaa gctcacaatc
      841 tgcgattgct cgccgttaac catactgtcg agctggctct ggatctgcgc ctgctccgcc
      901 accgagcctt tcaccagcgc gtcctgcgcg aggaaatttt gccgcgcatc gtcgatgtca
      961 cgttccgaga acggattcac cgccgcctgg ctgcctttca ggtaacgttg ataattttta
     1021 aacagacggt cgcgctccgc tgaaacctgg gtggtgttgg cctgcgcttc agtgagctgc
     1081 gcgcgcagcg tctttatatt atgcgtcgcc gtcatcaggt cagcctgaag tctgtcaact
     1141 cgcgcctggt aacgaaccgg gtcgagctta aaaagcacct cgcccttttg aataagctga
     1201 ttatttttgt cggtgacttc agtaacaatt cccgtcacct gtggcgtgat ggggatcgct
     1261 atcactgcct tttgcgcggt aaaagtgtaa gggtggttgt agttcatcaa caaaatcaaa
     1321 ccactcacca gaaacacgcc tcccagcgcc gcggtcgcca gcgtccactg attcaccgga
     1381 atgcggaaga ttttaaagac cgcccacgcc agcgccacgt aagttaaaac aatcaataga
     1441 tccatattta gatctccggg aacgtggaat agtcagtctt tttctcggcg gcgagttgat
     1501 gctccagccg ggaaattcgg tcagaaagtg cggcgatttc cgggtcagtc gctttctcct
     1561 gcgacgcaac atgtgactgc ataccccagc cacgctccgg ttgatagagc gtcgcccaga
     1621 tccacaggaa cggccagata acatgcagag taaacaggct cacccagccc gccgtatgaa
     1681 tagcgtcggc atggggatga ttgcgctttt tggcaatcag atatgggatg tcatgtatcg
     1741 cgatgattcc ataaaaaatc accagaaaga cgaagatcag cactcccaac gcgaaatagt
     1801 ttagaaacat atctgcctcg gattcacgtt tatcagtgtt gttttttggc tggcagccag
     1861 aagggagtca ggctgatatt ttgacaataa tccgggttcg cgattctcgc cataacacca
     1921 aagaataatt tttagaggtg atgagttgct tagttacata acgattttat gacgaaggca
     1981 taacatgctg tagatcacat caggtgaacg ccgtaagaaa atatcttgtg attcagatca
     2041 caaagatcca acaaaccacc aaaacaaaaa tgtgacatta gtcacattta aatgccattt
     2101 ttagcgaaaa tcgccgtctt gttgcttttt tacacaagca ttttgtgatg agcatcacgt
     2161 caattacctc tctaccccct atatttatgt gacccatatc acacaaaagg ccgtcgactg
     2221 gacagttaac cgattcagtg ccagatttcg cagtatctac aaggtccggc tacctctgcc
     2281 gccacattaa caaaaaacct cgggcttcca gcctgcgcga cagcaaacat aagaaggggt
     2341 gtttttatgt catccgatat taagatcaaa gtgcaaagct ttggtcgttt cctcagcaac
     2401 atggtgatgc caaatatcgg cgcgtttatc gcgtggggta tcatcaccgc gttatttatt
     2461 ccaacagggt ggttaccgaa cgagacgctg gcgaagctgg tcgggccgat gatcacttat
     2521 ctcctgccgc tgctgatcgg ttataccggt ggtaagctgg taggcggcga acgtggcggc
     2581 gtagtcggtg ccatcaccac catgggcgtt atcgtcggcg cagacatgcc gatgttcctc
     2641 ggttctatga ttgcaggtcc gctgggcggc tggtgcatta agcacttcga ccgctgggta
     2701 gacggtaaga tcaaatccgg ttttgagatg ctggtgaata acttctccgc aggcatcatc
     2761 gggatgatcc tcgctattct ggcattcctc ggcattggcc cgattgttga agccctgtcc
     2821 aaaatgctgg ctgcgggcgt taacttcatg gttgtccatg acatgctgcc gctggcgtct
     2881 atctttgttg aaccggcgaa aatcctgttc ctcaacaacg ccattaacca cggtatcttc
     2941 tcgccgctgg gcattcagca gtcccatgaa ttgggtaaat ccatcttctt cctgattgaa
     3001 gctaacccag gtccaggtat gggcgtgctg ctggcgtaca tgttctttgg tcgtggcagt
     3061 gctaaacaat ctgcgggcgg tgcggcaatc atccacttcc tgggtggtat ccacgaaatt
     3121 tacttcccgt atgtgttgat gaatccgcgt ctgatcctgg ccgttatcct cggcggtatg
     3181 actggcgtgt tcacgctgac tatcctgggc ggtggtctgg tttctccggc atctccgggt
     3241 tctatccttg ctgtactggc gatgacacca aaaggtgctt atttcgctaa catcgcaggt
     3301 gtgtgtgcgg cgatggctgt ctccttcgtt gtctctgcca ttctgctgaa aaccagcaaa
     3361 gtgaaagaag aagatgatat tgaagcagca actcgtcgta tgcaggacat gaaagctgag
     3421 tctaaaggtg cgtctccgct gtctgcgggc gatgtgacta acgacctgag ccacgtacgt
     3481 aaaatcatcg ttgcctgtga cgccggtatg ggttccagtg cgatgggcgc aggtgtgctg
     3541 cgtaagaaaa ttcaggatgc aggtctgtcg cagatttcgg tcactaacag cgcgatcaac
     3601 aacctgccgc cagatgtgga cctggtcatc actcaccgtg acctgaccga acgcgctatg
     3661 cgccaggttc cgcaggcaca gcatatttcg ctgaccaact tcctcgacag cggcttgtac
     3721 accagcctga ccgaacgtct ggttgctgcc cagcgccata ctgaaaacga agtaaaagtg
     3781 aaagacagtc tgaaagacag ctttgacgat tccagtgcta acctgttcaa gctaggcgcg
     3841 gagaacatct tcctcggtcg caaagcggcc accaaagaag aagcgattcg ttttgctggc
     3901 gagcagctgg tgaaaggcgg ttacgttgag ccagaatacg ttcaggcgat gctggatcgt
     3961 gaaaaactga ccccgactta tctgggtgag tctatcgcgg tgccacacgg tacggttgaa
     4021 gcaaaagatc gcgtactgaa aacgggcgtg gtgttctgcc agtacccgga aggcgtgcgc
     4081 ttcggtgaag aagaagatga cattgcccgt ctggtgattg gtattgctgc ccgtaacaac
     4141 gagcacattc aggttatcac cagcctgacc aatgcactgg atgatgaatc cgtcatcgag
     4201 cgtctggcac acaccaccag cgtggatgaa gtgctggaac tgctggcagg tcgtaagtaa
     4261 tacaatctca ccctctccac atggagaagg tggggcttat tgcctgatgc gctacgctta
     4321 tcaggcctac aaaacaaagc acagtttgtt gaatttgcac gttcttgtag gccggataag
     4381 gcgtttacgc cgcatccggc gctgcccctc tccacatgga gaagatgggc taattgcctg
     4441 atgcgctacg cttatcaggc ctacaaaaac aaagcacaat ttgttgaatt tgcacgttct
     4501 tgtaggccgg ataaggcgtt tacgccgcat ccggcgcggc ccctctccac atggagaggg
     4561 tttgggtgag ggaaaagcct caccccagcc ctctcgggta aaaacattga tgaaggttaa
     4621 tactatgaaa gcattacatt ttggcgcagg taatatcggt cgtggcttta tcggtaaact
     4681 gctggcagac gcgggtatcc aactgacgtt tgccgatgtc aatcaggtgg tacttgatgc
     4741 cctgaatgcc cgtcatagct atcaggttca tgtggtcggt gaaaccgagc aggtagatac
     4801 cgtttccggc gtcaatgccg tcagcagcat tggtgatgat gtcgttgatc tgattgctca
     4861 ggttgattta gtcactaccg ccgttggccc ggttgtgctg gaacgtattg ctccggcaat
     4921 cgccaaaggg ctggtgaaac gtaaagaaca aggtaatgaa tccccgctga acatcatcgc
     4981 ttgtgaaaac atggtccgcg gcaccacaca gctgaaaggc catgtgatga acgccctgcc
     5041 agaagatgcc aaagcgtggg tagaagaaca cgttggcttt gtcgattccg ccgttgaccg
     5101 catcgtgccg ccttcggctt ctgcaaccaa cgatccgctg gaagtgacgg tagaaacatt
     5161 cagcgaatgg attgtcgata aaacgcagtt caaaggcgcg ctgccaaaca tccctggcat
     5221 ggaattaacc gacaatctga tggcatttgt cgaacgtaaa ctcttcaccc tgaatacagg
     5281 tcatgctata accgcgtacc tcggaaaact ggccggtcat cagaccattc gtgatgcgat
     5341 tctcgacgag aaaatccgcg cggtggtaaa aggtgcgatg gaagaaagtg gtgcagtatt
     5401 gatcaagcgc tacggctttg acgcagacaa acatgcggcg tacatccgga aaatcctcgg
     5461 tcgtttcgag aacccgtacc tgaaagatga tgtagagcgc gtaggccgtc agccgctgcg
     5521 taaactgagt gctggcgacc gtctaatcaa gccactgctc ggaacgctgg aatatggtct
     5581 gccacataaa aacctgattg aaggtattgc cgctgcaatg cacttccgca gtgaagatga
     5641 tccgcaggct caggaactgg cagcactgat cgctgacaaa ggtccgcagg cggcgctggc
     5701 acagatttcc ggtcttgatg ccaacagcga ggttgtatcc gaggcggtaa ccgcttataa
     5761 agcaatgcaa taatggtgga ccaggcgcag gacaccctgc gcccgaataa cagattgtca
     5821 gatatgcagg caacaatgga acaaacccag gcctttgaaa accgtgtgct tgagcgtctg
     5881 aatgctggca aaaccgtgcg aagctttctg atcaccgccg tcgagctttt gaccgaggcg
     5941 gtaaatcttc tggtgcttca ggtattccgc aaagacgatt acgcggtgaa gtatgctgta
     6001 gaaccgttac tcgacggcga cggtccgctg ggcgatcttt ctgtgcgttt aaaactcatt
     6061 tacgggttgg gcgtcattaa ccgccaggaa tacgaagatg cggaactgct gatggcattg
     6121 cgtgaagagc taaatcacga cggcaacgag tacgcgttta ccgacgacga aatccttgga
     6181 ccctttggtg aactgcattg cgtggcggcg ttaccaccgc cgccacagtt tgaacctgca
     6241 gactccagtt tgtatgcaat gcaaattcag cgctatcaac aggctgtgcg atcaacaatg
     6301 gtcctttcac tgacagagct gatttccaaa atcagcttaa aaaaagcctt tcaaaagtaa
     6361 gtactttcgc ttacttctcc tctacctgtt tcggacgata aagctgacga taatactcca
     6421 gacgttgtag atataacggc acgctttcat caggtattcc ggacggaatc gcgttacggg
     6481 gaggtagttt tttcagatac tcccggaacg cctggctcga tgccatgaaa tctacggctt
     6541 tatcgataag aagcggaacg ttttcaccta atttgcccat gatgatatct ccgtattacc
     6601 ccgcgccggg aatgcgcggc cgccaatttt agtttagatc ccggtataaa tgacattaag
     6661 gaaaacgtgc tcaatcctca ataatcacct gcttctgttg actatctttt ttctttaaat
     6721 ttcttaatta atcaataaat tgcgtagttt ttaagcatgt taatgtttac gttatagaac
     6781 tattgcaatg tcatcagttg tgaacaaagc acctggtcgc gcatactagg ggctataaat
     6841 ttatctttat cagaagccat cacatgaaag aagtcgaaaa aaacgaaatc aaacgtctca
     6901 gcgatcgtct ggacgccatc cgccaccagc aggccgatct gtcgctggtt gaagccgcag
     6961 acaaatatgc cgaactggaa aaagagaaag ccactctgga agcagaaatt gctcgcctgc
     7021 gcgaagttca cagccagaaa ctcagtaagg aagcacaaaa gctgatgaag atgccattcc
     7081 agcgcgcgat taccaaaaaa gagcaggctg atatgggcaa gctgaagaaa agtgttcgcg
     7141 gactggtcgt tgtgcaccca atgaccgcgc tgggccgcga aatgggcctg caagagatga
     7201 cagggttctc aaagactgcg ttttaagttc ctttctcgtc gctgtaatca cagcctctca
     7261 ccatttactc gctcctgctg gcacatgcag gagcctgttt cagagatcga atattt
//
LOCUS       DAZFLD010000206         7279 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.207, whole genome shotgun sequence.
ACCESSION   DAZFLD010000206 DAZFLD010000000
VERSION     DAZFLD010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7279)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7279)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7279
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.207"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            80..1636
                     /locus_tag="ACHKBL_004052"
     CDS             80..1636
                     /locus_tag="ACHKBL_004052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012884439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HFO5820847.1"
                     /translation="MHQYLLFLLGLTPLLAMIILILKVKMPIHYAVLSTLFITLILAT
                     LFWHTPVQDLGSAVVYGAIKGLWPIVIVILAAIYSYNLMNATKSMNILRNVLAAVSDD
                     KRIQVLLISWCFGGFLEAAAGYGTAVAIPIGILIALGFNPLKAAIASLVANTVPTAFG
                     AVGIPVSVLAEQVNLPVTTLSGTIILQLAAFNILLPFVIVAIIGEGLKAIRGVGFMTL
                     MCGVSTLIPQYLVAVYLGAELPAFAGSLVSLIVIVFMAKNRKEKTAPEFLIETSADSA
                     SALSYSRGALLRACSIYILIFLFILLCSPLFPTVKSTVSQIASSLSFSLSNGSNLSMK
                     IEWIATPGVLIIIASVIGGWIQKASLSCLLNVFVSTVKQLKNSIIAISAIVAMATVMD
                     VCGLIGTLAQSLVDLTGGAYLFIAPVIGALGTFVTGSDTNSNILFGKLQTTAAEKLHV
                     DPLWLAAANTSGATGGKMISPQSIAIAVSATRMDGQGSVIMSGTLKYCFAYIVILGLK
                     VGLVYHLFMA"
     gene            1686..2405
                     /locus_tag="ACHKBL_004053"
     CDS             1686..2405
                     /locus_tag="ACHKBL_004053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HFO5820848.1"
                     /translation="MNVNFFVTCIGDAFKSRMAQNSVLLLEQLGCNVHFPEKQGCCGQ
                     PAINGGYVRDAIPGMKNLISSLEENDWPVVSPAGSCTYTIKNYARYLSEEPEWAARAE
                     KVAERLHDLTSFIVNHLGITDVGARLNGRAVYHPSCSLSRKLGVTREPLLLLENVRGL
                     ELLPFHAQETCCGFGGTFSVKMAEISGEMVKEKVGHIMDVKPDFLIGADASCLLNIGG
                     RLSREGHPVKVMHIAEVLMHR"
     gene            2416..3831
                     /locus_tag="ACHKBL_004054"
     CDS             2416..3831
                     /locus_tag="ACHKBL_004054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001544484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LutB/LldF family L-lactate oxidation iron-sulfur
                     protein"
                     /protein_id="HFO5820849.1"
                     /translation="MSLKTSDIRFKERIRIQIQDPIMRKAVANAQERIGTNRQKMVDE
                     LGHWEEWRSLAEQIRRHVLENLDAYLYQLSEKVTANGGHVYFARTREEATSYILKVAK
                     EKNARKVVKSKSMVTEEIGMNGVLQKAGIPVIETDLGEYILQLAHEPPSHVVVPAIHK
                     DRHQIRRVLHEHLGYDGPETPEAMTLFIREKIRQDFLSAEVGVTGCNFAVAETGSVCL
                     VTNEGNARMCTTLPKTHIAVMGMERIAPTFREVDVLITMLARSAVGARLTGYNTWLTG
                     PREEGHVDGPEEFHLVIVDNGRSEVLGSEFSDVLRCIRCGACINTCPAYRQIGGHGYG
                     SIYPGPIGAVISPLLGGYEDFKELPYACSLCTACNGVCPVKIPLAQLIQKHRQVMAES
                     EITPSTERRITRIFNYVNSHPALWKVGMIAGAKTASLCIRNGKTPISPGAIKEWSVAR
                     DLPSPDGESFRSWFKRHKKGG"
     gene            3836..4531
                     /locus_tag="ACHKBL_004055"
     CDS             3836..4531
                     /locus_tag="ACHKBL_004055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HFO5820850.1"
                     /translation="MENREEFLVAIARKLGRPVRRIPEAMPEPVSTLATTRLTELTMD
                     QRCEAFIKFASEVMLAKCVLVSPEDAPSKALDICWTFGSGPVIISNDQRLFDTGIAPL
                     LQKEMGAVLWTPEKGEENIHLAEKAKTGVVFAEYGLTESGGVVLFSAPERGRSVSLLP
                     EASVFVLRKSTILPRVAQLAQKLHDMAQEGQRMPSCINLIGGPSSTADIELIKVVGVH
                     GPVHATYLIIDDC"
     gene            complement(4711..>7279)
                     /locus_tag="ACHKBL_004056"
     CDS             complement(4711..>7279)
                     /locus_tag="ACHKBL_004056"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009035 - type I site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HsdR family type I site-specific
                     deoxyribonuclease"
                     /protein_id="HFO5820851.1"
                     /translation="SKESFNSENSLFKYLQLFVISNGTDTRYFANTTKRDKNSFDFTM
                     NWAKSDNTLIKDLKDFTATFFQKHTLLNVLVNYSVFDSSQTLLVMRPYQIAATERILW
                     KIKSSFTAKNWSKPESGGYIWHTTGSGKTLTSFKAARLATELDFIDKVFFVVDRKDLD
                     YQTMKEYQRFSPDSVNGSENTAGLKRNLDKDDNKIIVTTIQKLNNLMKAESDLPVYNQ
                     QVVFIFDECHRSQFGEAQKNLKKKFRRYYQFGFTGTPVFPENASGSETTASVFGRELH
                     SYVITDAIRDEKVLKFKVDYNDVRPQFKSLETETDEKKLSAAENQQAFLHPMRIQEIT
                     QYILNNFRQKTHRTFPGSRGFNAMLAVSSVDAAKVYYATFKRLQEEAANKSANYKPLR
                     IATIFSFAANEEQNAIGEISDETFDTGAMDSSAKEFLDAAIREYNDHFQMNFSTDSKD
                     FQNYYRDLAQRVKNQDVDLLIVVGMFLTGFDAPTLNTLFVDKNLRFHGLMQAFSRTNR
                     IYDATKTFGNIVTFRNLEQATIDAITLFGDKNTRNVVLEKSYAEYMEGFTDAATGEAK
                     RGFMAVVSELEQRFPAPASIESEKEKKDFVKLFGEYLRAENVLQNYDEFATLKALQQI
                     DLSDPVAVEKFKAEHYVDDEKFAELQMIRLPAERKIQDYRSAYNDIRDWQHREKEADK
                     KEKSTTDWDDVVFEVDLLKSQEINLDYILGLIFEHSRQNKSKDEMTVEVKRLIRSSLG
                     NRAKEGLVVDFIQQTNLDDLPDKASIIDTFFTFAQREQQREADALIKEENLNEEAARR
                     YIRTSLKREYATENGTELNETLPKLSPLNPQYKTKKQTVFQKISAFIEKFKGVGGHL"
BASE COUNT         1854 a         1593 c         1734 g         2098 t
ORIGIN      
        1 cctgaaaatt acaaattctc gcctgacagg gcaataacaa cagtaatgat gctgaatttg
       61 ttttcgttga ggaaatgaca tgcaccaata cctgcttttt ttactgggac ttaccccact
      121 tctggcaatg atcattttga ttctgaaagt caaaatgccc atacattatg ccgtactcag
      181 cacactcttt attacactga ttcttgcaac tttgttctgg cacacaccag ttcaggatct
      241 gggttctgct gttgtatatg gcgcaatcaa agggctgtgg ccgatagtga tagtcatact
      301 tgctgccatt tacagctata acctgatgaa tgcgacaaaa agcatgaaca ttctgcgaaa
      361 tgttctggcc gctgtcagtg atgataaacg tatccaggtg cttcttatct catggtgttt
      421 tggtggattt cttgaggcgg cggccgggta cggtacagct gtagcaatcc ccattggtat
      481 actcattgca ctgggcttta accctcttaa agccgcaatt gcatcattgg ttgctaatac
      541 agtacctacc gcgtttggtg ctgtcggcat acctgtatca gtacttgctg aacaggtaaa
      601 tttacccgta acaaccctca gcgggacgat tattcttcag ctggcagcgt tcaacattct
      661 gcttcccttc gttatagtcg ctattattgg ggaaggcctg aaggctatac gaggcgttgg
      721 gttcatgacc ttaatgtgcg gtgtgagcac attaatccca cagtacctgg tggctgttta
      781 tcttggcgca gaacttcctg ctttcgcggg aagtctggtc agcctgatcg tgatagtctt
      841 tatggcaaaa aaccgtaaag aaaaaacagc tcctgaattt cttattgaga cttcagcaga
      901 tagcgccagc gcactttcat actctcgtgg ggcgttgtta cgtgcgtgct caatctacat
      961 cctgattttt ctgttcattc ttctttgctc tccactgttc ccgacggtaa aaagtacagt
     1021 gtctcagatt gcctcgtcac tcagcttctc tctgagtaac ggcagcaatc tgtcgatgaa
     1081 gattgaatgg atcgcaaccc ccggtgttct gattatcatt gccagtgtta ttggcggctg
     1141 gatacagaaa gcatccctgt catgcctgtt aaatgtattt gtcagtacgg taaaacagct
     1201 caaaaactcc attattgcca tttctgcgat tgttgctatg gcaacggtaa tggatgtttg
     1261 tgggttgatt ggcactctgg cacagagcct tgtagatctc actggtgggg cctacctctt
     1321 cattgctccg gttatcggcg ctcttggtac atttgttact ggcagtgaca caaactccaa
     1381 cattctgttt ggtaaattgc agaccactgc ggctgaaaaa ttgcatgtgg acccactctg
     1441 gctggctgcc gctaacacat ctggcgcaac aggcggcaaa atgatttctc ctcagagtat
     1501 tgctatcgct gtttcagcca cccgtatgga tggtcagggg agcgtaatca tgtcagggac
     1561 actgaagtac tgctttgctt atattgttat tcttggactg aaagtaggcc ttgtctacca
     1621 cctgttcatg gcctgagaaa aaataacaaa cggacgtgga atattaatca acaggagaaa
     1681 tatctgtgaa tgttaacttt ttcgtcacct gcattggtga tgcatttaaa tcccgtatgg
     1741 cacaaaacag cgtgctgtta ctggagcaat tgggttgcaa tgtccatttc cccgaaaaac
     1801 agggatgttg cggtcagcca gcgataaatg gcggttatgt tcgtgatgcc attcctggta
     1861 tgaaaaatct catatcttcg ctggaagaga atgactggcc ggtagtatct cctgccgggt
     1921 catgcactta cacgataaaa aattacgctc gctatctcag tgaagaacct gagtgggcgg
     1981 cacgagctga aaaagtagct gaacgcctgc atgatttgac gtcatttata gtaaatcatc
     2041 ttggcatcac agatgttggg gcaagactca atggtcgcgc agtgtaccat ccttcatgca
     2101 gcctttcccg taaactgggt gtaacccgag aaccgctttt gttgcttgag aatgtacggg
     2161 gacttgagct gctgccgttt cacgctcagg aaacgtgctg cgggtttggc ggcacgttct
     2221 ctgtaaaaat ggcggagatc tctggtgaaa tggtgaaaga gaaagtcggg catattatgg
     2281 atgttaaacc tgattttctt atcggggctg acgccagttg cctgctcaac atcggcggtc
     2341 ggcttagccg tgaagggcat cctgtaaaag tgatgcatat tgctgaagtc ctgatgcaca
     2401 gatgaggtaa acagaatgtc tttgaaaaca agtgatatca ggtttaaaga gcgtatccgt
     2461 attcagattc aggacccgat catgcgaaaa gctgtggcta acgcacagga gcgcataggg
     2521 actaatcgac agaaaatggt tgatgaactg gggcactggg aagaatggcg ttcacttgct
     2581 gaacagattc gtcggcatgt tctggaaaat cttgatgctt atttgtatca attgtccgaa
     2641 aaagttactg cgaatggcgg acatgtatat tttgcccgta ccagagagga ggcaaccagt
     2701 tacatactga aggtggcgaa agaaaaaaac gccaggaagg ttgttaaatc caaatcaatg
     2761 gtgactgagg aaattggtat gaacggcgtt cttcagaaag ccgggatacc agtgatagaa
     2821 acagatcttg gtgaatatat tcttcagctg gcgcatgagc cgccatcgca tgttgttgtg
     2881 ccggcaatac ataaagatcg ccatcaaatt cgcagagttt tgcatgaaca tctggggtat
     2941 gacggccctg aaactccaga agcaatgaca ttgttcatcc gtgagaaaat cagacaggat
     3001 tttctgagtg ctgaagttgg cgtaacaggg tgtaactttg ccgttgcaga aacaggctca
     3061 gtttgccttg tgacaaacga aggtaacgca cgtatgtgca ccacgttacc taaaacacat
     3121 attgctgtga tgggtatgga gcgtattgca ccaacattcc gggaggtgga tgttcttatc
     3181 accatgcttg caaggagtgc tgtgggagca agactgacag gttataacac ctggcttaca
     3241 ggtccaagag aagaaggaca tgttgacggc ccggaagagt tccatctcgt cattgttgac
     3301 aatgggcgtt cagaggttct cggctcagaa ttcagcgatg tattgcgctg tattcgctgc
     3361 ggtgcctgta ttaatacctg tcccgcttat cgccagattg gtggacatgg ctatggctca
     3421 atctacccgg gcccaatcgg agcagttatt tccccgttgc ttggtggata tgaagacttt
     3481 aaggaattac cttatgcctg ctcattgtgc actgcctgca atggggtatg tccggtaaaa
     3541 atacctctgg cacaactaat acaaaaacat cggcaggtta tggcagagtc cgaaataaca
     3601 ccatcaacag aacgccgtat aaccagaata ttcaattacg taaacagtca tccggcattg
     3661 tggaaagttg gcatgattgc tggcgcgaaa acagccagcc tgtgcatcag gaacggaaaa
     3721 acgccgatta gcccaggtgc tattaaagaa tggtctgtgg ccagagactt accttctcct
     3781 gatggagaaa gcttccgtag ttggttcaaa agacataaaa aaggagggta actttatgga
     3841 aaacagagag gaatttctcg ttgctatcgc cagaaagctt ggcaggccag taagacgtat
     3901 tcccgaagca atgccggagc ctgtaagtac gctggctacg acacgtttaa ccgagttgac
     3961 aatggaccaa cgctgtgagg cttttattaa atttgcctca gaagtgatgc ttgcgaagtg
     4021 cgttctggtt tcgcctgaag acgcaccatc aaaagcatta gacatctgtt ggacgttcgg
     4081 ttccgggcca gtcattatca gcaacgatca acgactgttt gatacgggga ttgcaccatt
     4141 gctacaaaaa gagatgggcg ctgtcctgtg gaccccggag aagggtgagg aaaatattca
     4201 tcttgcagaa aaagcaaaaa caggcgtcgt tttcgctgag tatggactga cagaatctgg
     4261 tggtgttgtt ttattctccg cccctgaacg agggcgttca gtaagtcttt tacctgaagc
     4321 gtctgtgttt gttttacgca aaagtaccat tcttcccaga gtggctcagc ttgcgcaaaa
     4381 acttcatgat atggctcagg aaggacaacg tatgccttcc tgtatcaatc tgattggagg
     4441 cccaagttct actgcagata tagaactgat caaagttgtt ggtgtgcatg gacctgtaca
     4501 tgcaacttac ttgattattg atgactgcta atacatttta gtatgtatat atgattaaca
     4561 gctccgttta gctgagctgt taatcattca tttagttgta ttagtcgtga cttgagcgta
     4621 agcgtcaata cggactccgt taatgcctta caatagcagt cagtgttcat gcctgtttat
     4681 tgaggcaagg gggagtccgg gcgcctgtaa ctaaagatgc ccccccacgc ctttaaactt
     4741 ctcaataaat gcgctaatct tctgaaaaac ggtctgtttt ttcgttttat attgcgggtt
     4801 taacggacta agttttggta atgtctcgtt taattctgtg ccattttcgg tggcgtattc
     4861 gcgttttaac gacgtgcgaa tatagcgcct ggccgcttct tcattaagat tttcttcttt
     4921 gatcaatgca tctgcttcac gttgttgttc gcgttgagca aaggtaaaga acgtatcaat
     4981 gatgctggct ttgtccggca aatcatcaag gttcgtttgc tgaataaaat cgaccaccag
     5041 gccttcttta gcacggttcc ccagacttga acgaattaat cgtttgacct ctacggtcat
     5101 ttcgtcctta cttttgtttt gtctgctgtg ttcgaaaatc aggccaagga tataatccag
     5161 gtttatttcc tgagacttca gcaaatcgac ctcaaaaacc acgtcatccc agtcagtggt
     5221 tgatttttct tttttgtcag cttctttttc gcggtgctgc cagtcacgaa tatcgttata
     5281 ggcagaacga taatcctgaa tcttgcgttc agcagggaga cgaatcattt gcaattcagc
     5341 gaacttttca tcatccacat aatgttctgc tttgaatttt tctaccgcaa caggatcgct
     5401 gagatcgatt tgttgcaggg ctttcagcgt ggcaaattca tcatagtttt gcaggacgtt
     5461 ctcggcacgc aggtattcac caaacagttt aacgaagtct tttttctctt tttcgctttc
     5521 aatactggca ggggcaggga acctttgttc cagttctgaa actactgcca taaagccgcg
     5581 tttagcttcg ccagtggcag catctgtaaa gccttccata tactctgcat aactcttttc
     5641 cagcaccaca tttctggtgt ttttatcacc aaaaagtgtg atggcatcga tggttgcctg
     5701 ctccaggtta cggaatgtga cgatgttacc gaaggttttc gtggcgtcat aaatgcggtt
     5761 ggtacgggaa aacgcctgca tcagaccgtg aaaacgcagg tttttatcga cgaacagcgt
     5821 gttcagtgtt ggagcatcga agccggttaa aaacatcccc acgacgatta acaggtcaac
     5881 atcctgattt ttaacccgct gagccaggtc ccgatagtag ttctgaaaat ccttgctatc
     5941 agtactgaaa ttcatttgaa aatggtcgtt atattcacga attgcagcat ccagaaactc
     6001 tttagcactg ctgtccattg cgccggtatc aaaagtttca tcggaaattt caccaatggc
     6061 attttgttct tcattggcgg caaaggagaa tattgtcgca atacgcagtg gtttataatt
     6121 tgcagattta ttagcggctt cctcctgtaa gcgtttaaac gtcgcgtaat agactttcgc
     6181 agcatccacg ctgcttactg ccagcatggc attaaaacct ctggaacccg ggaaagtgcg
     6241 gtgggtcttc tggcggaagt tattcaggat atattgcgtg atttcctgaa tacgcatggg
     6301 atgaagaaac gcctgctgat tttcagccgc actcagtttt ttctcgtcag tttctgtctc
     6361 taaagactta aactgtggcc gcacatcgtt gtagtccacc ttgaatttga gcactttttc
     6421 gtcacgaatc gcatcggtaa tcacatacga atgcaattca cgaccaaata cgctggcggt
     6481 cgtttctgaa cctgaggcgt tttccgggaa aacaggtgtg ccggtaaaac caaactgata
     6541 atagcgtctg aatttcttct tcagattttt ctgcgcttct ccaaactggc tgcggtggca
     6601 ttcatcaaat ataaacacca cttgctgatt gtatacaggc aggtcgcttt ctgctttcat
     6661 caggttatta agtttctgaa tagtggtgac gataattttg ttatcgtcct tatccagatt
     6721 tcgtttaagg cctgcggtat tttccgagcc gttaacacta tctggcgaaa aacgctgata
     6781 ttccttcatg gtctggtaat cgaggtcttt cctgtcgacc acaaagaaga ctttatcaat
     6841 aaagtccagt tctgttgcca gacgcgcggc tttaaagctg gtcagtgttt taccggaacc
     6901 cgtggtgtgc cagatatacc caccgctttc cggttttgac cagttcttcg ctgtaaagga
     6961 gctcttaatt ttccacagaa tgcgctcggt ggcagcaatc tggtacggtc gcatcaccag
     7021 tagcgtctga ctgctgtcaa aaacgctgta gttcaccaga acattaagca gggtatgttt
     7081 ctggaaaaag gtggcggtaa aatctttgag gtctttaatc agcgtgttgt ctgatttcgc
     7141 ccagttcatg gtgaagtcaa aactgttttt atcgcgcttt gtcgtgttgg caaagtaacg
     7201 ggtatcggta ccgttggaaa tgacgaacag ttgcagatac ttaaacaggg aattttcgct
     7261 gttaaaactc tccttgctg
//
LOCUS       DAZFLD010000207         7240 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.208, whole genome shotgun sequence.
ACCESSION   DAZFLD010000207 DAZFLD010000000
VERSION     DAZFLD010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7240
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.208"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..724
                     /gene="traW"
                     /locus_tag="ACHKBL_004057"
     CDS             <1..724
                     /gene="traW"
                     /locus_tag="ACHKBL_004057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001189538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="conjugal transfer protein TraW"
                     /protein_id="HFO5820852.1"
                     /translation="GIHAGYCTEAEYARFGGTDVCPAVGELPGGDSQVRSLYQGAGTA
                     DTPAALTWDQKQIDAATAYMKNTARPSAGRAPGKGEVGTQTGRTYVGLQNEYNGIIDA
                     ASHPQLSLIADSTPNEATRGALTEALQSPSAAAYFDRTASSEARTRGHMSQREFEAFE
                     AGRRYANTDWQQDLQGMEGDNLLRELLRTTALLNWQMNDLKEQIRQGNVIAGQQLALA
                     ARQYYGQRLGELSQAMSQGSVR"
     gene            721..1290
                     /gene="traX"
                     /locus_tag="ACHKBL_004058"
     CDS             721..1290
                     /gene="traX"
                     /locus_tag="ACHKBL_004058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000650756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraX"
                     /protein_id="HFO5820853.1"
                     /translation="MKDDNSTDKKKPAPRLRRIGDIGINLFAPLAETRWMLRSCRHML
                     GQHKTRLKRLMPDRQPESPVCMTWEEAVRASGLTVNELDRRLRRRRMFWRTGFWLMMI
                     PVLLLCGMALAATALPAITLIRILACLLLMSAIAALCASKTLIVSYRLWQLNERRVSE
                     DEQGTFPDYLQARHGWKQATWLAITHRQY"
     gene            1363..3522
                     /locus_tag="ACHKBL_004059"
     CDS             1363..3522
                     /locus_tag="ACHKBL_004059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DotA/TraY family protein"
                     /protein_id="HFO5820854.1"
                     /translation="MKIIPRALSAGLALAAVPAQAFVTYQDIVSAATNPDDLSRQALV
                     TIFGDVVTNPLTASSNTIIGNLFMVFNAIIAIMGIVWFLFIGIRHVVRTGHQGNVFNN
                     GRDVVGMLSIVSGFMMIVPTASGWSLAQLVMLWGASIMGIGSANIMVQTAAENIAEGY
                     SMTVQPVHVSTRTAARGILEMALCKHAMNAGLDDFNRTARSSTPPMSESSRTENGRYT
                     VTVSNGSATCGSVSLSVKGNGSNNQSALDYFFNPFIETEYQGVLTAQRSAMDTMLRDL
                     DSAAQEFVSAYRTRRDTGNGNLPDIESRIQKAANRYEETVQKALPTDNAETERREQLK
                     YYLTTYGWVALGAWYQTFATANQRLAELADRAPTVSGVSSQGEAGDTEFLKEVKGAYQ
                     AQLQNSPYTPALGTTASSTEYRTAEAAEPKSIIMKVWSGVNFADKIATSLSGTGTTSD
                     QLNPLIKMKNIGDYTMVTTETIWAGYTTVRVLSTAGSNSIFGRLVNAVSGLSDAAKAF
                     LEAAAPPLYFLLFLLFCAGFSLSIYLPFIPFIFWMTGIGNWIVSVLIGCAAGPLWAAT
                     HLGTNQDRGSRAAYGYIYLIDSMIRPLLMVFGFFFASVAIVAGGTILNALFGAALVNV
                     QADSLTGLFSIAGFLMIYARACTTLVAAVFALQAYLPDHVINFLGGRDGVNMLGNMVS
                     SVKDIIAGGNTNIRRTPGVREEKLKNINGDDKDGIKQ"
     gene            3604..4251
                     /gene="excA"
                     /locus_tag="ACHKBL_004060"
     CDS             3604..4251
                     /gene="excA"
                     /locus_tag="ACHKBL_004060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid IncI1-type surface exclusion protein
                     ExcA"
                     /protein_id="HFO5820855.1"
                     /translation="MNTTKSRYPSWWAYYQLARNTYFIFGIPCLLFYAFFGTLIVHDS
                     SLSQTRDFIFAYGCWALIILPLLWLYLKTRAKKKKVQQVVQQIKESSPFAPTNDYEQL
                     SFSKSCYFGIDIKNGTMLYVRIYPNNVMDVIGLDIHNFTRTVAEDGKLEIHTTYVSLP
                     MIPLEISGISARTLANTIHTMAARGYEYKERFPQMIRYRVKEWEKVAGVPVAEVF"
     gene            complement(4565..4717)
                     /locus_tag="ACHKBL_004061"
     CDS             complement(4565..4717)
                     /locus_tag="ACHKBL_004061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001618166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFO5820856.1"
                     /translation="MPQRTFLTMLIVVCVTILCFVWMVRDSLCGFRVEQGNTVLVATL
                     AYEVKR"
     gene            5050..5292
                     /locus_tag="ACHKBL_004062"
     CDS             5050..5292
                     /locus_tag="ACHKBL_004062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001317518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5820857.1"
                     /translation="MSNEYQSTSINPDVFEQFLTLLNTHCKSSEAALAMLSTLCYELM
                     RRSGIHSLDSLMLNGEGILIQLQGADIREKTGQHIH"
     gene            complement(5385..6920)
                     /locus_tag="ACHKBL_004063"
     CDS             complement(5385..6920)
                     /locus_tag="ACHKBL_004063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016242774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFO5820858.1"
                     /translation="MSCIKEKSKYAFERAKQFYFMLWCKESLIYLLFSVLVTWCCGYT
                     VKNALGNVLILFLMMLLGRRNVISATCVCIFLLLSAFYAPVGMIYGKINNSFIASVMQ
                     TNINEAGEFITGIPVLYFIVSLVIAAFTFWFWRKRDKSHRGFIFLLFFIVLSVNAWPK
                     RMVTNVMTGIAETREEMMRYESLKNNQKDSWDIVSVEKKYKTIIIVIGESVRRDYLSV
                     YGYPLPTTPWLNSAPGIFINGYFSAAPNTIGSLSRTLTLDYTETGNPGNNIVTLARKA
                     GYETWWISNQGSLGQHDTLISVIAANADKKHFLKEGSSSSLRIDDEDMLQYVREAVNS
                     SSPKVIFIHMLGSHTNPCDRLFNDAQPFRELFGHKVSCYLSTLLKLDRFLQAIYNMTT
                     EHGDDFAMLYFSDHGQSVSSDSTPVHHAPGLRSGYDVPLMMMASDITEHHLNHVHISA
                     RRFPEIFQWITGIQTKNLPLSFSLLQNDFAESVSVFNGERDVPVMSLPEEPILTGNAE
                     SEK"
BASE COUNT         1983 a         1850 c         1665 g         1742 t
ORIGIN      
        1 aggcattcat gccggttact gcacggaagc tgaatatgcc cgctttggcg gaaccgatgt
       61 ctgccctgcc gtgggcgagc tgccgggcgg agacagccag gtgcgatcgc tctatcaggg
      121 tgcaggcaca gcagataccc ctgccgccct cacctgggat cagaaacaga ttgatgctgc
      181 cacggcttac atgaaaaaca ccgccagacc gtcagcgggt cgtgccccgg ggaaaggtga
      241 agtgggaaca cagaccgggc gcacgtatgt gggcctgcag aatgaataca acggcatcat
      301 cgatgccgct tctcatccgc aactgtcact gatagctgac agtaccccta atgaggccac
      361 ccgtggagcg ctgactgaag ccctgcagtc accatccgct gcggcatatt ttgaccggac
      421 cgcatcatca gaggcccgca cccgtggtca catgagccag cgggagtttg aggcctttga
      481 agctggcaga cgctatgcaa acaccgactg gcagcaggat ctgcagggca tggaaggcga
      541 taaccttctc cgtgaactgc tgcgcactac cgccctgctg aactggcaga tgaacgacct
      601 gaaagagcag atacgccagg gtaatgttat cgccgggcag cagctggcac tggcggcacg
      661 acagtattac ggacagcgtc tcggtgaact gtcacaggcc atgagccagg ggagcgtaag
      721 atgaaagacg acaacagcac cgacaaaaaa aaaccggccc cccgtctccg ccgcatcggt
      781 gacatcggga taaacctgtt cgcaccactg gcagaaaccc ggtggatgct gcgcagctgc
      841 cgccatatgc tgggccagca taaaacgcgc ctgaagcgcc tcatgcccga ccggcaaccg
      901 gagagtccgg tctgtatgac ctgggaagaa gccgtcaggg ccagcggcct tacggtcaac
      961 gaactggatc ggcgtctgcg acgtcgcagg atgttctggc ggacaggatt ctggctgatg
     1021 atgataccgg tacttctgct gtgcggcatg gcccttgctg ccacagcgct tccggccatc
     1081 accctgatac gtatactggc ctgcctgtta ctgatgagtg ccattgcagc actgtgtgcc
     1141 tcaaaaaccc tgattgtgtc ataccgcctc tggcaactga atgaacggcg ggtcagcgag
     1201 gacgaacagg gcaccttccc cgattacctg caggcccggc acggctggaa acaggccaca
     1261 tggctggcca ttacccaccg gcagtactga ccgtccggtc tgcaattcct tttcctcatt
     1321 ccggaggctg atgccctgca tcccttcggt tccggagtta tcgtgaaaat catacccaga
     1381 gcactgagtg cgggtctggc gctggctgcc gtacctgcac aggcattcgt cacctatcag
     1441 gacattgtga gcgccgccac caatccggat gacctgtcgc gccaggcact ggtcaccatt
     1501 tttggcgacg tggtcacaaa tcccctgaca gccagcagca acaccattat cggtaacctg
     1561 ttcatggtgt ttaacgcaat tatcgccatt atggggatag tctggttcct gtttattgga
     1621 atccgccatg ttgtccgcac cggccaccag ggcaatgtct ttaacaacgg acgggacgtc
     1681 gtcggaatgc tcagtattgt gagcggtttt atgatgattg tgcccaccgc ttccggctgg
     1741 agcctggcac aactggtcat gctctggggc gcatccatca tgggcattgg ttccgccaat
     1801 atcatggttc agactgctgc agaaaacatc gctgaaggct attcaatgac cgtacagcca
     1861 gttcatgtct ccacccgcac ggcagcacgg ggaatactgg agatggcgct gtgcaaacac
     1921 gccatgaatg caggcctcga tgactttaac cgtactgccc gttcatcaac gccccccatg
     1981 agcgaatcct cacggacaga gaacggcagg tataccgtca ccgtttcaaa tggcagtgcc
     2041 acctgcggga gtgtgagtct ctccgtgaaa gggaatggca gcaacaacca gtccgcactg
     2101 gactattttt tcaatccctt cattgaaacg gagtatcagg gtgtcctgac ggcacaacgc
     2161 tccgccatgg ataccatgct ccgtgacctg gacagcgcag cacaggaatt tgtgtctgcg
     2221 taccggaccc gacgcgacac gggtaacggc aacctgccgg atattgaaag ccgtattcag
     2281 aaggctgcca accggtatga agaaacggtg cagaaggcgt tgccaacaga taacgcagaa
     2341 actgagcgca gggagcagct gaaatactac ctgaccacct acggctgggt tgccctgggg
     2401 gcctggtacc agacctttgc caccgccaac cagcgactgg cagaactggc cgaccgtgcc
     2461 cccactgtga gtggagtttc atcgcagggc gaagccggag atactgaatt cctgaaggaa
     2521 gtaaaaggag cttaccaggc acagttgcaa aactcaccat atacacctgc actgggaaca
     2581 accgcttcat caactgaata cagaacagca gaagctgctg aacctaaaag cataatcatg
     2641 aaagtatggt cgggtgtaaa ttttgcagac aaaattgcaa catccttatc tggaacagga
     2701 accacctcag accaactaaa tcctctgata aaaatgaaaa atatcggtga ttacactatg
     2761 gtcacaacag aaaccatctg ggcgggttat accaccgtca gggtactttc cactgccggc
     2821 agcaactcta tttttggtcg actggtcaat gccgttagcg gcttatctga tgccgcaaag
     2881 gcttttctgg aagcagctgc accaccgctc tacttcctgc tgtttttgct gttctgtgcc
     2941 ggtttttcgt tatccattta cctgccgttt attccgttta tcttctggat gaccggcatc
     3001 ggtaactgga tagtcagtgt actgattgga tgcgccgccg gtcccctgtg ggctgccacc
     3061 catctgggga caaaccagga ccggggaagc cgggctgctt acggttacat ctacctgata
     3121 gacagcatga tcagaccact gttgatggtg ttcgggttct tttttgcatc ggtcgccatc
     3181 gtggctggcg gcaccatact gaatgcgttg tttggcgcgg ctctggttaa tgtgcaggct
     3241 gactccctta cagggttatt cagtatcgcg ggatttttga tgatttatgc ccgtgcctgt
     3301 accacgctgg ttgcggccgt ttttgcgctt caggcttatc tgcctgacca tgtaattaat
     3361 tttctgggcg gacgtgacgg cgttaatatg ctggggaata tggtttcatc cgtcaaagat
     3421 attattgccg gtggcaacac caatatacgc agaacacctg gcgttcgtga agaaaaactg
     3481 aaaaacatta atggagatga taaagacgga ataaaacaat aaagtacaga catcaaaaaa
     3541 aacagtattg ccacattcac ctgaagagat taaaatacat tcaggtgaat atggaggttt
     3601 attatgaaca caacaaaaag cagatatcct tcctggtggg catattatca actagcccgc
     3661 aatacttact ttatatttgg aattccatgc ttattattct atgctttttt cggaacacta
     3721 attgttcatg actcaagctt atctcaaaca agagatttta tttttgcata tggatgctgg
     3781 gcattaataa ttctcccatt actctggcta tatctgaaaa ccagagcgaa gaaaaagaaa
     3841 gtccagcagg ttgttcagca gataaaagaa agcagtccct ttgcaccgac caacgattac
     3901 gagcaactgt cattctcgaa gagttgttat ttcgggatcg acattaaaaa cggcaccatg
     3961 ctgtatgtgc gcatttaccc aaacaatgtc atggatgtga ttggcctgga tattcacaac
     4021 ttcacccgaa cagttgctga agacggcaag ctggaaatcc acaccaccta tgtgagtctg
     4081 ccgatgatcc ctcttgaaat ctccggcatc tcagcaagga cgctggccaa caccatacac
     4141 accatggctg cacgtggata tgagtacaaa gagcgtttcc cgcagatgat ccggtatcgg
     4201 gttaaagagt gggaaaaagt tgccggtgtt cctgttgcag aagtattttg aggtagctat
     4261 caactgttag acgataatcc gtcagccggt tttctctgaa ccggcttttt cttttcaggc
     4321 agaatgcctg tctgccgtcc ctgtatcaga ctgtaattag cacaatacgg caatttgccg
     4381 ttcagtatat tcaggaagta caaatcatgc catctgcaac acaaactgcc cgactggaag
     4441 caaggatcag cacagaactg catgctatgc tgaaacgcgc tgcagaaatc caggtaaaaa
     4501 agggcgcatt gaggtttgct ttcgcaacaa tccggcggca gatttcctgc cgccgtgttg
     4561 cccattaacg tttaacttcg taggctaacg ttgccacaag cactgtgttt ccctgctcga
     4621 cacggaatcc gcaaagcgaa tccctcacca tccagacaaa acacagtatc gtcacacaga
     4681 cgacaattaa cattgttaaa aacgttcgtt gtggcatcgc agcccctcca ttgcctttcg
     4741 gccgtcatcg ggctactata atgttgtcta ggcattacag tgggccctgg gttgattgat
     4801 aaaaaaatcg cctggggctt tctgctgcat gccgtccaca aaacgctttc agcatacagc
     4861 ctcaagcgcc cgccgctatt ctaatcgatt ttcagtctgc cacaatcgtt ttccacctaa
     4921 tgacatgtta tgcacaacag tatttttacg ggaaactaca cggcaaggat tcccggaaca
     4981 cgttcattat ttgatggtta cagacatcat gttaaatccg gaagtaataa tgacctgaaa
     5041 ggaaatacta tgagtaacga atatcaaagc accagtataa accctgatgt ttttgaacaa
     5101 tttcttaccc tcctgaatac acactgtaaa tcatcagaag ctgcacttgc catgctgagt
     5161 acactctgct acgaactgat gaggaggagt gggatccatt cactggacag cctgatgctc
     5221 aacggagaag gtatattgat acaattacag ggggctgata ttcgcgaaaa aacaggtcag
     5281 catattcact gatccaggga tgtgctgaaa acagtgtcag cctgaaataa acgttcaatg
     5341 cctcatacac atgaggcatt gagcacggtc atcaataatc tttattattt ttctgattca
     5401 gcatttccgg tcagtatcgg ctcttctggc aaagacatta ccggaacgtc acgttcacca
     5461 ttgaatacag aaacactttc agcgaaatca ttctgtaaca aagaaaaact taacggcaga
     5521 tttttggttt gtatccctgt gatccattga aaaatctcgg gaaaacgacg tgcactgata
     5581 tgcacatgat tgaggtggtg ctcagtaata tcactggcca tcatcatgag aggtacatcg
     5641 tatcccgaac gcaaacctgg cgcatgatgc acaggggtgc tgtctgaact gacggattgc
     5701 ccatgatcag aaaaatacag catggcaaaa tcatcaccat gctctgtcgt catgttataa
     5761 attgcctgaa gaaaccgatc cagtttaagc agtgttgata aataacagga tactttatgt
     5821 ccgaataatt ccctgaatgg ctgtgcatca ttaaaaagtc ggtcacaggg attagtgtga
     5881 gaccccagca tatgaataaa aatcactttt ggagaagacg agttaacagc ttccctgaca
     5941 tactgaagca tatcctcatc atcaatgcga agggaagaag agctcccttc cttaagaaaa
     6001 tgttttttat ccgcattggc ggcgatgaca gaaatcagcg tatcatgttg ccctaaagag
     6061 ccctgatttg atatccacca cgtctcatac ccggcttttc tggcaagcgt aacaatatta
     6121 ttacccggat tacctgtttc agtgtaatca agcgtcagtg ttctgctgag tgaacctatc
     6181 gtattcggcg ctgctgagaa atacccatta ataaatatac ccggagcaga attcagccat
     6241 ggtgttgtcg gcaaaggata cccataaaca gaaagataat cccgtctgac actttcacca
     6301 attacgataa ttatggtttt atatttcttc tccacactca caatatccca tgagtctttc
     6361 tgattgtttt tcagcgattc atatcgcatc atttcttctc tggtttcagc tatccctgtc
     6421 attacattcg taaccatacg ttttggccat gcatttactg acaatacgat aaagaacaac
     6481 agaaagataa atccccggtg gcttttatca cgcttacgcc agaaccagaa tgtaaaagca
     6541 gcaatgacca gcgaaacaat aaaataaaga acaggtatgc ctgtgataaa ctcacctgcc
     6601 tcatttatat ttgtctgcat tacagacgca ataaaactgt tgtttatttt cccgtaaatc
     6661 atcccaaccg gagcatagaa tgcacttaac aaaagaaaga tacagacaca cgttgctgaa
     6721 ataacattcc gccgccccaa caacatcatc aggaacaata taagcacatt ccccagggcg
     6781 tttttcactg tatatccgca gcaccatgta acaagtacag aaaacaacag ataaatcagt
     6841 gactctttac accacaacat aaaataaaac tgttttgctc tctcaaaagc atatttcgac
     6901 ttttctttaa tacaagacac gccagtcatt ttcctgtaca aacacagacg atcatttcaa
     6961 cgcatggata tttatatgaa acagttcaca aaaataccct accctgtcca gcagggttta
     7021 catattatca ggttctgtgt ttacagcaac tgctccccga accagattat tcgcataacc
     7081 cgatgaaggc ctcataacca tcaaacactc agaatacatt tagttaacaa aaatatgtct
     7141 tgccctcccg gaaaggcaaa catacgaaag agaaagcatg gctataataa tatcaatcaa
     7201 aatatgctca atctttccgg ttgccataca ctcagcaccg
//
LOCUS       DAZFLD010000208         7218 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.209, whole genome shotgun sequence.
ACCESSION   DAZFLD010000208 DAZFLD010000000
VERSION     DAZFLD010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7218
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.209"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(832..2265)
                     /locus_tag="ACHKBL_004064"
     CDS             complement(832..2265)
                     /locus_tag="ACHKBL_004064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfjI family protein"
                     /protein_id="HFO5820859.1"
                     /translation="MCLLAPENPYPIYALPPLVRNAIIETQQNTQAPLAMVATSALTA
                     MSIACQNQIDVCRPGNLRGPVNIYSLILADSGERKTTVDKVFMKAFYLRDEALAEEYA
                     KLVENYSTEKEIWEQKQKALESKFHKEIRAGKDYKATESELETHLNNSPVPPQIRRTI
                     FNDTTIEGMLKYYSDSNRSFALVSSEGGVIFDSRAMSKLGIINTLWDGGSLFIDRKSS
                     PGINLKEPRLTMSAMIQPDVYHKGFCTRKKELVKTSGHHARFLMCQPTSMQGTRIITG
                     DNYSSQYQELFDKRINELIDESLAMSGERRCLHFSPQAARIWTDYYNDVESKLGGLGP
                     LRHCREYAAKNAEYMARLAGLIYHSSGEEGEISPYTAEMARELAIWYGNEYMRLSNPL
                     TFDNTALTVPVRLIPEELELFNWIKSYCIEKGILCMKKNDILQRGPNRFRKKDKINWL
                     LDLLYEQNRVVPVIEGKTLCVAPNFDL"
     gene            complement(2361..2588)
                     /locus_tag="ACHKBL_004065"
     CDS             complement(2361..2588)
                     /locus_tag="ACHKBL_004065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000270113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO5820860.1"
                     /translation="MWVPLTDEQRSVILKAYGTEGDRLVREKERKEITSISRSQAWKL
                     EREGNYPPRKTIGKKSCGWLLSDLLWWIQTR"
     gene            3318..4931
                     /locus_tag="ACHKBL_004066"
     CDS             3318..4931
                     /locus_tag="ACHKBL_004066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5820861.1"
                     /translation="MVNQTMQQNQYYRILSGESRPFPGIYEAVSEQLLLLIFKARQLY
                     QDPEYRPWFEEQHKVLLALRDNTELQKIFNKQRKENFALGASTSSQPTALTNALNVMC
                     HLYNGFDEGNISLKSWGAMQEVQTTPDFFSLGPRTWKTPELAKFVGDDLFNILTECKA
                     WSSPIPAEARQGYCHQDLSVAHSCTSELLAHLKQYHEIDESTILAITIGLAVNEFYQY
                     KQNIPEWLSCFENKNKWPWDCRKSDTEDVHRLRERLSIIGCTEFVKNMFSVASQHEIK
                     TMYNIYVHIYAAEICGWVKTSMAYFKPTDFIQYTWKFATVTPDKDEKTENTTAWERDY
                     CQITIQRDELLRLIRTNTLQDAAIHLSGCLQYAYLESVKNDIDYPGIAPNAEEKKMKY
                     TDSIEFSGIRNNIINAVKRLESFEKWYETNKVLLVSNKTIRTEKIDVVVRLAGLKAYD
                     LQEGVPDGLKWKVKDGINERIKTDTSLCLPQDISDTSLNRYRHAVTRIVNDEIDTLLM
                     EQKKKNKRFPYSKDRHAIQPLWSKCVIEQ"
     gene            complement(5024..5620)
                     /locus_tag="ACHKBL_004067"
     CDS             complement(5024..5620)
                     /locus_tag="ACHKBL_004067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5820862.1"
                     /translation="MAVIPMSYSPATVARRFSILDGVTIQGVLYQIIWDPKTPFAAVI
                     EAAPSVIDGDIRHKVVATLELQRRSQLEGVFVRKFWEEQDVAQIEGIVVDGAVRDVGL
                     ATFVYETVATKAGVILLSDNEQYEGGKALWQHIARRSTNLKVFILDTDSARYYPFDGD
                     RICYDGESIPESEIWSEHPDRNKHGVVLVAESVNGKAA"
     gene            complement(5750..7066)
                     /locus_tag="ACHKBL_004068"
     CDS             complement(5750..7066)
                     /locus_tag="ACHKBL_004068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO5820863.1"
                     /translation="MAVNLTETAIRALKAKKTSFYVWSNSSQRGTGRLGVKVQSSGSK
                     IFYFRYYVEKGTKERFIQLGIWPEMKLATANELAKKYGAWLIEGKDPQTELERQQLAE
                     QQRRQLHQSQGSFEALIHGYVNKMKIDNKRTWQDVLKRLENECYTVIPRETKAKDVTS
                     GQIKHILAGIIQRGAVVHSNRIRSYLMAAFNYGLKADNDPMNTSVGISFGLEANPVSV
                     IPKQSSAEKVGDTWLTLEELRFLMEHFAEATNVGLLMQHLIRLCIYTGGQRPFEMIAS
                     QWCDVDFQQKTLLVTADVSKNKREHLIPLTESALQELSCVQELTKEKSSPYIFPLSTN
                     GKRPVRTDSLARAIMYFRACNPDFKIFTARDLRRTCKTLMGEAGISKEIRDRIQNHAL
                     NDVSSKHYDRYDYLPEKRRALEIWEDRVNNYQQQTGNNVVNLFGRR"
BASE COUNT         2244 a         1599 c         1372 g         2003 t
ORIGIN      
        1 taaaagagat aaccagcagt acagacaacc tgttaaaact gttttgtatt acctcattac
       61 agcaatgagg taatacttca gaatacagtg agactcactt aaactgagga ccagtacgaa
      121 ttacaatggt aaaaacacac ctcatagaat taagtttaag gaagtaccat tcaatcactt
      181 acaggaagta atctgacatt cacacaagtc agtatcgggt agctacaggc aacaccgcta
      241 ttgatacccc acacaccata agaagattat ttcatacaga aaagattata tttacccata
      301 cttataaact gtaacgctac tataacgtac gaaccaaaga taatatatcc ctaaagtgtt
      361 ctggtacgta actagtgtta aatacgaacc agacacacac tcaatggatt aactttctca
      421 atattacgct atgagtgagt gacataattt acacataact aatccccata caccaccaca
      481 ctgtagcata tggggataga cgcattacgc acatacagaa tactcagcat aaagaggagt
      541 gacattacga tagaaagaga caacataaca gataacataa ctcccattaa taatactaat
      601 taacctaaat tatttattga atacgaaaac aatgaagtgc cctccttatc taaggccaaa
      661 gccagcaata tcctgtacaa cactctatcc aatagtaaat agtagttaca ccagttatat
      721 ttactgactg acctgtacca aagttctgta atacaacact gacatggtca tggcattaat
      781 ctcctccgca agtaacggag gagactatat gttactcaac gattaagtcg ttcagaggtc
      841 aaagttaggt gcgacacaca acgtttttcc ctcaataacc ggtacaactc tgttttgttc
      901 atacagtaaa tccagtaacc agttgatttt atcctttttc cggaaacggt tcggaccacg
      961 ctgtaaaatg tcattttttt tcatacagag gatccccttc tcaatgcaat agctttttat
     1021 ccagttgaaa agttcaagct cctctggaat gagtcgcaca ggtacggtca gagcagtgtt
     1081 gtcaaaagtt aacggattag acaaccgcat atactcatta ccgtaccata ttgctaattc
     1141 tctcgccatt tctgcagtgt aaggggaaat ctccccctct tcaccgctcg aatggtaaat
     1201 aagtcctgcc agtcttgcca tatactctgc atttttggca gcatattccc gacaatgtct
     1261 taaaggcccc aatcccccca gtttcgattc cacatcattg taataatccg tccagattct
     1321 ggcagcctga ggagaaaagt gaaggcaacg tcgttcacca ctcatcgcca gactttcatc
     1381 aataagttca ttgatcctct tatcaaataa ctcctgatac tgtgatgaat aattatctcc
     1441 ggttattatc cttgtcccct gcattgatgt tggttgacac atcaaaaacc ttgcatgatg
     1501 tcctgacgtt ttcacaagtt ctttttttcg cgtacaaaaa cctttgtggt aaacatcagg
     1561 ctgaatcatc gccgacatcg tcagtcttgg ctccttcaaa ttaattccgg gagatgattt
     1621 cctgtcgatg aaaagagaac ctccatccca caaagtgtta ataattccca gtttactcat
     1681 ggcccggctg tcaaaaatta ctcccccttc actggataca agagcaaaag aacgattgct
     1741 atcggagtaa tattttaaca ttccctctat cgttgtatca ttaaaaattg ttcgacgtat
     1801 ctgcggcgga acaggagagt tattcagatg cgtttcaagc tctgattctg ttgccttgta
     1861 atctttaccg gcacgaatct ctttatgaaa ttttgattcc agcgcttttt gtttttgctc
     1921 ccatatttcc ttttctgtac tgtaattctc aaccagtttc gcgtattctt ccgccagggc
     1981 ttcatccctg agataaaatg ctttcataaa cactttatcc acggtcgttt tcctttcacc
     2041 ggaatcagcc agaatcagag agtaaatatt aacgggccca cgtaaatttc caggtctgca
     2101 cacgtcaatc tgattctgac aggcaattga catcgctgtt aatgcggatg tcgccaccat
     2161 agccaaagga gcctgtgtat tttgttgagt ttcaattatt gcatttctca ccagcggtgg
     2221 tagtgcatat atcggataag gattttctgg tgctagtaag cacataaagc ctccattttc
     2281 attaatgggt tagtaaagtt gcagcaattt ccgctacaat agcgaaaata gctgctattc
     2341 agactgaaaa tacaaacagt tcatctggtc tgaatccacc agagaaggtc acttagtaac
     2401 cagccacaag atttctttcc gatagtctta cgcggtgggt agtttccctc tcgttcaagt
     2461 ttccaggctt gtgaacggga aattgaggtg atctcttttc gttccttttc acgtaccagt
     2521 ctgtcaccct cagtcccata ggctttcaaa attactgaac gttgctcatc tgttaatgga
     2581 acccacatga cggccctcct gatatatcaa tgattcttat ctcagttgtg agcatgaaat
     2641 gactgaattt cctggacaat ccatagtgga gattccgttg tcatattgct cacagaacac
     2701 ccatggacag caaggtacat acgaaaacaa atcaagtcaa gttaagacaa ataaaacaat
     2761 acttgactca caagcccact cataggtcaa tatgtgttga ttagcaacaa aacaaccaat
     2821 aatgcacatt acaaacaatg attttaagcc aagaagtggt tttcactgtt gacaatgaca
     2881 caaaatgaat ctagtatcga cttcatgtta tgctcagata aacttctgac agtgatagta
     2941 aagaagagaa ggagggggtt aaatttgtga ccaagatcac aaaaataaat tagtttttac
     3001 tattcacaat aggagggaaa gagagccagg cattttacct gtcattgaaa tgacaacaac
     3061 agcttcaaca gatcggaata ttcaggagga ggaagaagaa ggagatctga cgggtgttat
     3121 aattgttaca acacgctgga ggaaaattat cgtaataata gatgcattct caggtatacc
     3181 ctgtatcacc ccgaaaacac ggcaggcatc cgttttatta gtagatgcag actcagtatc
     3241 aacgtatcct ttcaggagat aacatcgatt ttaactgcca gtatccggta tgcggcaaaa
     3301 ggaaaccttc ttacataatg gtaaatcaga ctatgcaaca aaatcagtac tatcgtattt
     3361 tatccggaga atcccgccca ttccctggca tatatgaggc tgtttccgaa caactccttt
     3421 tgctcatttt taaggcacgt cagctatatc aggatccaga atatcgcccc tggtttgaag
     3481 aacagcacaa agttctcttg gcattgcgtg ataatacaga gctccagaaa atttttaaca
     3541 aacaaagaaa agaaaatttt gctcttggcg catctacatc gtctcaaccc actgctctta
     3601 ctaatgcgct taatgttatg tgccatttat acaatggctt tgatgaagga aatatcagcc
     3661 tgaaaagctg gggagcgatg caagaggtac agacaacacc agactttttc agcctgggac
     3721 caagaacctg gaaaacacct gaactggcaa agtttgtagg agatgattta tttaatattc
     3781 tgactgaatg taaagcctgg agttcaccaa ttccagcaga agcccggcag gggtattgcc
     3841 atcaggatct gtctgttgcg cactcctgca cctcagagtt gctggcacac ctgaagcagt
     3901 atcatgaaat agatgaatca acaatacttg ccataaccat tggtctggcg gtcaacgaat
     3961 tctaccagta taagcaaaat attccagaat ggctttcctg ttttgagaat aagaacaaat
     4021 ggccctggga ctgcaggaag tcagatactg aagatgtgca cagactgaga gaacgactat
     4081 cgataatcgg atgtacagaa ttcgtaaaaa atatgttctc agtcgccagt cagcatgaaa
     4141 taaagacaat gtacaatatt tatgtccata tttatgcagc tgaaatctgc ggttgggtaa
     4201 aaacaagcat ggcatatttt aaaccaacag atttcatcca gtacacatgg aaattcgcaa
     4261 cagttactcc ggataaagat gaaaaaaccg agaatacgac cgcatgggaa agggattatt
     4321 gtcagataac catacaacga gatgaactcc ttagattgat tcgcactaat actctgcagg
     4381 atgcagcaat tcatctttca ggctgcctgc aatatgcata tctggagtct gtaaagaatg
     4441 atattgacta tcctggaata gcccctaatg ctgaagaaaa gaaaatgaaa tacacagatt
     4501 ctattgaatt ttctggaatt aggaataaca tcataaatgc agtaaaacgg ctggaatcat
     4561 ttgaaaaatg gtatgaaact aataaagttt tgttggtaag taataaaaca atacgaacag
     4621 agaagattga cgttgttgta cgacttgcgg gactgaaagc ctatgatttg caggaagggg
     4681 ttcctgatgg tctgaaatgg aaagttaaag atggcataaa tgaacgcatc aaaaccgata
     4741 ccagtttatg cctgcctcag gatatcagtg atacatcgct aaaccgttat cgtcacgctg
     4801 tgacgcgcat tgtcaacgac gaaatcgaca cattactgat ggaacagaag aaaaaaaata
     4861 aaagattccc ctacagcaaa gatcgacatg cgatccaacc tttatggagc aaatgcgtga
     4921 tcgagcagta gagttctgag gggataaccg agctgttatc ccccgtttat tctgccaact
     4981 ctcaggtctg tcagagttcc aagaatacga tgcggctggc aaattatgcg gcttttccat
     5041 taacagattc ggccacaaga acaacaccgt gtttatttcg atcaggatgt tcactccaga
     5101 tttctgattc aggaatactt tccccgtcat agcagatccg atcgccatca aacggataat
     5161 accgtgcgga atcagtatct aaaataaaca ctttcagatt cgttgaacga cgcgctatat
     5221 gctgccagag ggctttacct ccttcatatt gctcgttatc acttaatagt ataacgcccg
     5281 cttttgtggc taccgtttca tatacaaacg tcgcaaggcc aacatcacgt acagccccat
     5341 caacaacaat cccttcaatc tgagcgacgt cctgctcttc ccagaatttc cggacaaaca
     5401 ccccttcaag ctgtgagcga cgctgaagct ccagagtggc aacaacttta tggcgaatat
     5461 caccatcaat aacagaaggt gcagcttcga ttaccgcagc gaacggagtc tttggatccc
     5521 agatgatctg gtaaagcacg ccctgaatag ttactccatc cagtatcgaa aaacgacgag
     5581 ctacagtagc aggggagtaa gacattggaa ttacagccat ttttgaatcc cctctgagta
     5641 atacaggcac gaaaatttac ctcaataatt atagtaaaca atacaagaac taggtgcatc
     5701 aacaataccc atcaattgta cgcaattcgt acagtaacca ggaactccct caccttctgc
     5761 caaacagatt gacaacatta ttgcctgttt gctgttgata gttattaacc cgatcctccc
     5821 agatctcaag tgctctgcgt ttttctggca aataatcgta tcgatcataa tgttttgaac
     5881 tgacatcgtt caacgcatga ttttgaatac gatcgcggat ttccttactg attccagctt
     5941 ctcccattaa cgttttacag gtccgacgta agtcacgtgc tgtaaaaatt ttaaagtcag
     6001 gattacaagc ccggaaatac atgatagcgc gagccagact gtccgtacga accggacgct
     6061 taccattagt cgaaagcggg aaaatgtaag ggctcgattt ttcttttgtt aactcctgca
     6121 cacaggataa ttcctgtaat gctgattcag tcaacggtat caaatgctct cgtttatttt
     6181 tagagacatc cgcagtcacc agcagcgttt tttgctgaaa atcaacatca caccactgac
     6241 tggcgatcat ttcaaaggga cgttgccctc cggtatagat acataatcga atcaaatgtt
     6301 gcatcaataa tccaacgttg gttgcttcag caaaatgttc cattagaaaa cgcaactctt
     6361 cgagtgtcag ccaggtgtca ccgacttttt ctgcagatga ctgtttcggt atcactgata
     6421 caggattggc ctccagccca aaactaatcc ctacacttgt gttcatcgga tcgttatcag
     6481 ctttcagacc atagttaaat gcagccatca ggtaagaacg gatccggtta gagtgaacca
     6541 cagcaccgcg ctgaatgatc ccggccaaga tatgtttaat ctgtccggaa gtcacatctt
     6601 tagctttagt ctcacggggg atcactgtat agcattcatt ttcaaggcgt ttcagaacat
     6661 cctgccatgt tctcttatta tcaattttca ttttgttaac ataaccatga atcaatgctt
     6721 caaatgaacc ctgtgattga tggagttgtc tccgctgctg ttctgcaagc tgttgtcgct
     6781 ccagttctgt ctggggatcc tttccttcaa tgagccaggc accatacttt tttgccagtt
     6841 cgttcgctgt ggccagtttc atctcaggcc atatacccaa ttgaatgaat ctttcttttg
     6901 tccctttttc cacgtaatag cggaaataga aaattttgct gccagaagac tgaactttga
     6961 cgccaagtcg tccagtgcca cgttgagagc tgttgctcca gacatagaac gatgtctttt
     7021 ttgcttttaa tgctcgaata gctgtttcag ttagattgac ggccatagaa cctcttttct
     7081 aaatcagtaa caatcaaaat aactcgtaaa tttgggtcag gtaatgggtc aggtaacgat
     7141 cgtacaatgg agaaaaaacg gaaccaagcg gaaacagaag aaattacata attagttgat
     7201 tataaacaat taataaaa
//
LOCUS       DAZFLD010000209         7212 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.210, whole genome shotgun sequence.
ACCESSION   DAZFLD010000209 DAZFLD010000000
VERSION     DAZFLD010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7212
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.210"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..479
                     /gene="curA"
                     /locus_tag="ACHKBL_004069"
     CDS             <1..479
                     /gene="curA"
                     /locus_tag="ACHKBL_004069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NADPH-dependent curcumin/dihydrocurcumin
                     reductase"
                     /protein_id="HFO5820864.1"
                     /translation="RHATEVLGFDVCLDHHADDFAEQLAKACPKGIDIYYENVGGKVF
                     DAVLPLLNTSARIPVCGLVSSYNATELPPGPDRLPLLMATVLKKRIRLQGFIIAQDYG
                     HRIQEFQKEMGQWVKEDKIHYREEITDGLENAPQTFIGLLKGKNFGKVVIRVAVDD"
     gene            677..1342
                     /gene="mcbR"
                     /locus_tag="ACHKBL_004070"
     CDS             677..1342
                     /gene="mcbR"
                     /locus_tag="ACHKBL_004070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid/biofilm transcriptional regulator
                     McbR"
                     /protein_id="HFO5820865.1"
                     /translation="MPGTEKMKHVSLTLQVENDLKHQLSIGALKPGARLITKNLAEQL
                     GMSITPVREALLRLVSVNALSVAPAQAFTVPEVGKRQLDEINRIRYELELMAVTLAVE
                     NLTPQDLAELEELLEKLQQAQEKGDMEQIINANRLFRLAIYHRSNMPILCEMIEQLWV
                     RMGPGLHYLYEAINPAELRERIENYRLLLAALKAKDKEGCRHCLAEIMQQNIAILYQQ
                     YNR"
     gene            complement(1378..3480)
                     /gene="pqqU"
                     /locus_tag="ACHKBL_004071"
     CDS             complement(1378..3480)
                     /gene="pqqU"
                     /locus_tag="ACHKBL_004071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor PqqU"
                     /protein_id="HFO5820866.1"
                     /translation="MKIFSVRQTVFPALLVLSPTVFAADEQTMIVSAAPQVISELDTP
                     AAVSVVDGEEMRLATPRINLSESLTGVPGLQVQNRQNYAQDLQLSIRGFGSRSTYGIR
                     GIRLYVDGIPATMPDGQGQTSNIDLSSVQNVEVLRGPFSALYGNASGGVMNVITQTGQ
                     QPPTIEASSYYGSFGSWRYGLKATGATGDGTQPGDVDYTVSTTRFTTHGYRDHSGAQK
                     NLANAKLGVRIDDASKLSLIFNSVDIKADDPGGLTEAEWKANPQQAPRAEQYDTRKTI
                     KQTQAGLRYERSLSAQDDMSVMMYAGERETTQYQSIPMSPQLNPSHAGGVITLQRHYQ
                     GIDSRWTHRGELGVPVTFTTGLNYENMSENRKGYNNFRLNRGVPEYGQKGELRRDERN
                     LMWNVDPYLQTQWQLTDKLSLDAGVRYSSVWFDSNDHYVTPGNGDDSGDASYHKWLPA
                     GSLKYAMTDAWNVYLAAGRGFETPTINELSYRADGQSGMNFGLKPSTNDTIEIGSKTR
                     IGDGLLSLALFQTDTDDEIVVDSSSGGRTTYKNAGKTRRQGAELAWDQRFAGDFRVKA
                     SWTWLDATYRSNVCNEQDCNGNRMPGIARNMGFASIGYVPEDGWYAGTEARYMGDIMA
                     DDENTAKAPSYTLVGLFTGYKYNYHNLTVDLFGRVDNLFDKEYVGSVIVNESNGRYYE
                     PAPGRNYGVGMNIAWRFE"
     gene            3722..4783
                     /locus_tag="ACHKBL_004072"
     CDS             3722..4783
                     /locus_tag="ACHKBL_004072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HFO5820867.1"
                     /translation="MHLRHLFSSRLRGSLLLGSLLVASSFSTQAAEEMLRKAVGKGAY
                     EMAYSQQENALWLATSQSRKLDKGGVVYRLDPVTLEVTQAIHNDLKPFGATINNTTQT
                     LWFGNTVNSAVTAIDAKTGEVKGRLVLDDRKRTEEVRPLQPRELVADDATNTVYISGI
                     GKESVIWVVDGENIKLKTAIQNTGKMSTGLALDSKGKRLYTTNADGELITIDTADNKI
                     LSRKKLLDDGKEHFFINISLDTARQRAFITDSKAAEVLVVDTRNGNILAKVAAPESLA
                     VLFNPARNEAYVTHRQAGKVSVIDAKSYKVVKTFDTPTHPNSLALSADGKTLYVSVKQ
                     KSTKQQEATQPDDVIRIAL"
     gene            complement(4896..6395)
                     /gene="ansP"
                     /locus_tag="ACHKBL_004073"
     CDS             complement(4896..6395)
                     /gene="ansP"
                     /locus_tag="ACHKBL_004073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparagine permease"
                     /protein_id="HFO5820868.1"
                     /translation="MSKHDTDTSDQHAAKRRWLNAHEEGYHKAMGNRQVQMIAIGGAI
                     GTGLFLGAGARLQMAGPALALVYLICGLFSFFILRALGELVLHRPSSGSFVSYAREFL
                     GEKAAYVAGWMYFINWAMTGIVDITAVALYMHYWGAFGGVPQWVFALAALTIVGTMNM
                     IGVKWFAEMEFWFALIKVLAIVTFLVVGTVFLGSGQPLDGNTTGFHLITDNGGFFPHG
                     LLPALVLIQGVVFAFASIEMVGTAAGECKDPQTMVPKAINSVIWRIGLFYVGSVVLLV
                     MLLPWSAYQAGQSPFVTFFSKLGVPYIGSIMNIVVLTAALSSLNSGLYCTGRILRSMA
                     MGGSAPSFMAKMSRQHVPYAGILATLVVYVVGVFLNYLVPSRVFEIVLNFASLGIIAS
                     WAFIIVCQMRLRKAIKEGKAADVSFKLPGAPFTSWLTLLFLLSVLVLMAFDYPNGTYT
                     IAALPIIGILLVIGWFGVRKRVAEIHSTAPVVEEDEEKQEIVFKPETAS"
     gene            6662..>7212
                     /locus_tag="ACHKBL_004074"
     CDS             6662..>7212
                     /locus_tag="ACHKBL_004074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HFO5820869.1"
                     /translation="MIKVHGVPGWGTTISEMMLTLADIPYQFVDVSGFDHEGTSRELL
                     KTLNPLCQIPTLALENDEIMTETAAIALMVLDRRPDLAPPVGRAERQQFQRLLVWLVA
                     NVYPTFTFADYPERWAPDAPEQLKNNVIEYRKSLYIWLNSQLTAEPYAFGEQLTLVDC
                     YLCTMRTWGPGHEWFHDNAQNISS"
BASE COUNT         1936 a         1793 c         1759 g         1724 t
ORIGIN      
        1 gccgccatgc tactgaggtg ctgggcttcg atgtttgtct tgaccatcac gcggatgatt
       61 ttgccgaaca actggcgaaa gcgtgcccaa aaggtattga tatctattat gaaaacgtgg
      121 gcggtaaggt atttgatgca gtgctacctt tacttaatac atctgcgcgc attcccgttt
      181 gtggcttagt gagcagctat aacgctacag agctaccacc cggtccggat cgtttacctc
      241 tgctgatggc tacggtgctg aaaaaacgta ttcgcttgca aggatttatt atcgctcagg
      301 attatggtca ccgcatccaa gagtttcaga aggagatggg gcaatgggtg aaagaggata
      361 aaatccacta ccgcgaagaa attactgacg gtttagagaa tgcgccacag acgtttatcg
      421 gcctgctgaa gggtaaaaac ttcggcaaag tggtgatccg cgtggcggtt gatgattaaa
      481 gtttcaacgg cggcgtaagc cgccataaaa atccaggcaa aaagcttatt ttcagcttta
      541 attaactaac cacgtcattg ttctgctgct aataacaaca aaggtgagtg gtttatgatt
      601 attcacttta atacaccagg tgaactcctt ctgccatgca ggcagggttg gacagaaaac
      661 acgcgaagga attaaaatgc ctggaacgga aaaaatgaaa catgtcagtt tgactctgca
      721 ggttgagaac gacctgaaac atcagcttag tattggcgca ttaaaacctg gcgcacgcct
      781 gattactaaa aatctggcgg agcaattagg tatgagtatt acacctgtgc gtgaagcatt
      841 attacgtctg gtttcggtga atgcgctttc tgtcgcacca gcgcaagcat ttacagttcc
      901 ggaagtgggg aaacgtcaat tagacgaaat caatcggatc cgctacgagc tggaattaat
      961 ggcagttact ctggctgttg aaaacctcac cccgcaagac ctggcggaac tcgaggaatt
     1021 gctcgagaaa ttacagcagg cgcaagaaaa gggcgacatg gaacaaatca ttaatgcgaa
     1081 caggctattt cgcttagcga tttatcatcg ttcaaatatg cccatcctgt gtgagatgat
     1141 tgagcaactg tgggtcagga tgggacctgg tttacattat ctctatgaag cgattaatcc
     1201 agcggaatta cgggagcgta tagaaaacta tcgtctatta ctcgccgcgt taaaagcaaa
     1261 agacaaagag ggatgcagac attgtcttgc tgaaattatg caacaaaata ttgctatttt
     1321 ataccagcaa tacaatcgtt aaattaaatg cccggcaaat accgggcatc aaggtaatta
     1381 ctcaaatcgc cacgcaatat tcatgccgac accataattt cgcccgggcg caggttcgta
     1441 atatcgcccg tttgactcat tgacaatgac agagccaacg tattctttat cgaataaatt
     1501 atcgacacga ccaaataaat ccacagttaa attgtggtaa ttgtatttat acccggtgaa
     1561 taagccgacg agagtataag acggcgcttt ggccgtattt tcatcatctg ccataatatc
     1621 gcccatataa cgcgcttccg tacctgcata ccagccatct tccggtacat aacctatcga
     1681 cgcaaagccc atattacggg cgatccctgg catccggtta ccgttacaat cctgttcatt
     1741 gcaaacattg ctgcgatagg tcgcatcaag ccaggtccag gacgctttta cgcgaaaatc
     1801 tcccgcgaaa cgttgatccc atgccagttc agcgccttga cgacgggtct ttccggcatt
     1861 tttgtaagtc gtacgcccac cgctactgct atcgacaaca atttcatcat cggtgtcggt
     1921 ctggaacaat gcgagactaa gcagcccatc accaatacgc gttttactgc cgatctcaat
     1981 tgtatcgttg gtggagggtt ttaaaccaaa gttcataccg ctttgcccat cagcgcgata
     2041 agacagctca ttaatcgtcg gcgtttcaaa accgcgcccg gctgccagat agacattcca
     2101 ggcatcggtc attgcatatt ttaacgatcc ggctggtagc catttgtgat aactggcatc
     2161 accgctgtcg tcgccgttac ccggagtaac gtaatgatca ttggaatcaa accacacaga
     2221 gctgtagcgc acgccagcat ccagcgaaag tttgtcggtc agttgccact gggtttgtaa
     2281 atagggatcg acgttccaca tcaaattgcg ttcgtcgcgg cgcaactcac ctttttgccc
     2341 gtattccggc acgccgcgat tcaggcggaa gttattgtaa cccttgcggt tttcactcat
     2401 gttttcgtag ttcaggccgg ttgtgaacgt gactggaacg cccaattcac cacggtgtgt
     2461 ccagcggctg tctatcccct ggtaatggcg ttgcagggta atcacgccgc ccgcatgtga
     2521 cgggttaagt tgcggggaca tggggattga ctggtactgg gtcgtttctc gctctccggc
     2581 atacatcatt acactcatat catcttgcgc gctcaggcta cgttcatagc gcaacccagc
     2641 ctgagtttgc ttgatggttt ttcgcgtgtc gtactgttct gcacggggcg cttgttgtgg
     2701 attcgccttc cattctgctt cggttagccc acctgggtca tctgctttga tatccacact
     2761 attgaaaatc aggcttaatt tgctggcgtc atcaatgcga acgcccagtt tggcattggc
     2821 taaatttttc tgtgcgccac tatgatcacg atagccgtgg gtcgtaaagc gcgtggttga
     2881 gacggtgtaa tcgacatcgc ctggctgtgt gccgtctccc gttgcgcccg ttgctttcag
     2941 cccatagcgc cagctgccaa aactgccgta gtaactactg gcttcaatgg ttggtggctg
     3001 ttgtccggtc tgggtgatga cattcattac accgccagac gcgttgccat acagagcaga
     3061 gaaggggcca cgcagcactt ccacattttg cacactgctt aaatcgatat tggatgtctg
     3121 cccttgtccg tcgggcatgg tagcgggaat accgtcaaca tacaggcgaa taccgcgaat
     3181 tccgtaagtg gagcgggagc caaatccgcg aatcgacagc tgtaaatctt gcgcatagtt
     3241 ctgccggttt tgtacctgca aaccaggcac gccagtcagt gattcagaca agttaatgcg
     3301 cggtgttgcc aggcgcatct cctcgccatc caccacgctc acggctgctg gggtatccag
     3361 ttctgaaatc acctgcggcg cggcactgac aatcatggtc tgttcatcag cggcaaaaac
     3421 ggtgggggaa aggacaagca gcgcgggaaa aacggtctgt cggacggaaa aaatcttcat
     3481 gaaaaaagcc aggttaagaa tgggaaaaca ccgtcatggt aataaaattg taaatttatg
     3541 gaaaacgaaa cggcacgata tgttaagtaa ttgagaaaat tgtagtcgta acggcaagaa
     3601 atgctccaca tttgagaaaa taatgattac cattcccatt tataacaaga gcgtaacgat
     3661 gattacgctt agcggagcat tgtgaagcag caaaaatatc ggttcatcaa agggagtcgt
     3721 catgcattta cgtcatctgt tttcatcgcg cctgcgtggt tcattactgt taggttcatt
     3781 gcttgttgct tcatcattca gtacgcaggc cgcagaagaa atgctgcgta aagcggtagg
     3841 taaaggtgcc tacgaaatgg cttatagcca gcaagaaaac gcgctgtggc tcgccacttc
     3901 gcaaagccgc aaactggata aaggcggcgt ggtttatcgt cttgatccgg ttactctgga
     3961 agtgacgcag gcgatccata acgatctcaa gccgtttggt gctaccatca ataacacgac
     4021 tcagacgttg tggtttggta acactgtaaa cagcgcggtc acggcgatag atgccaaaac
     4081 gggcgaagtg aaaggccgtc tggtgctgga tgatcgtaag cgcactgaag aggtgcgccc
     4141 gctgcaaccg cgtgagctgg tggcagacga tgccacgaac accgtttaca tcagtggtat
     4201 tggtaaagag agcgtgattt gggtcgttga tggcgagaat atcaaactga aaaccgccat
     4261 ccagaacacc ggtaaaatga gtaccggttt agcgctggat agcaaaggca aacgtcttta
     4321 caccactaac gctgacggcg aattgattac catcgacacc gccgacaata aaatcctcag
     4381 ccgtaaaaag ctgctggatg acggcaaaga gcacttcttt atcaacatca gccttgatac
     4441 cgcaaggcag cgtgcattta tcaccgactc taaagcggca gaagtgttag tggtcgatac
     4501 ccgtaatggc aatattctgg cgaaggttgc ggcaccggaa tcactggctg tgctgtttaa
     4561 ccccgcgcgt aatgaagcct acgtgacgca tcgtcaggca ggtaaagtca gtgtgattga
     4621 cgcgaaaagc tataaagtgg tgaaaacgtt cgatacgccg actcatccga acagcctggc
     4681 actgtctgcc gatggcaaaa cgctgtatgt cagtgtgaaa caaaaatcca ctaaacagca
     4741 ggaagctacc cagccggacg atgtgattcg tattgcgctg taatagatat ggcgagggtg
     4801 aaaatggaga gacttgcctg acttttatca cgccgtatcc ggcaccttcg tcagtaattt
     4861 gaatttgccc ggcacgtagc cgggcaattt tgcaattagc tcgccgtttc aggcttaaac
     4921 acaatttcct gtttttcttc atcttcctcg acgactggcg cagtgctgtg aatttcagca
     4981 acgcgtttgc gcacaccaaa ccagcctata accagcagaa taccgataat cggcagcgcc
     5041 gcgatagtat aagtcccgtt cgggtaatcg aacgccatca gcacaaggac gctcagcaaa
     5101 aacagtaatg tcagccagga agtgaagggc gcgccaggca gtttaaaact gacatccgct
     5161 gctttgcctt ctttaatcgc tttacgcagg cgcatctggc acacgatgat aaacgcccat
     5221 gaagcgatga ttcccagcga cgcgaagttc aacacaatct caaatacgcg cgacggcacc
     5281 agatagttga ggaatacgcc gacgacatac acaaccagtg tcgccagaat cccggcatac
     5341 ggcacatgct gacgactcat tttcgccata aaactcggtg cggaaccgcc catcgccatc
     5401 gagcgcagaa tacgtccggt gcagtacaga cctgagttca ggctggagag ggcagcggtc
     5461 agcaccacaa tgttcataat gctgccgatg tacggcacac ccagtttaga gaaaaacgtc
     5521 acgaacggac tttgccccgc ctgatacgcg ctccacggca ataacataac cagcaacacc
     5581 acggagccga cgtaaaacag gccaatacgc caaatcacac tgttaatcgc tttaggcacc
     5641 atggtctgcg gatctttaca ttctccggca gctgtaccca ccatttcaat ggaggcaaaa
     5701 gcaaacacta cgccctgaat caacaccagc gcaggtagca aaccgtgggg gaagaagccg
     5761 ccgttatcgg taattaaatg aaagccagtg gtgttgccat ccagcggctg accactaccg
     5821 aggaacactg tacccacgac caaaaaggtc acaatggcga gcactttaat aagcgcaaac
     5881 cagaactcca tctcggcaaa ccatttcaca ccaatcatat tcatggtgcc aacgatggtc
     5941 agcgcagcga gcgcaaagac ccactgcggt acaccaccaa atgcgcccca gtaatgcata
     6001 tacagtgcaa cggcggtaat atcgacaatc cccgtcatcg cccagttgat gaagtacatc
     6061 cagcctgcaa cataagcggc tttctcaccc aaaaactcac gggcataaga gacaaaactg
     6121 ccactggaag ggcggtgtag caccagctca cccaatgcac gcagaataaa aaacgaaaac
     6181 aagccgcaga ttaaatagac cagtgccagt gctggccccg ccatttgcag tcgggctccc
     6241 gcgcctaaaa acaaacccgt accaatcgcg ccgccaatgg cgatcatctg cacctggcga
     6301 ttgcccatcg ctttgtgata cccctcttcg tgggcattaa gccagcggcg tttcgcggcg
     6361 tgctgatctg aagtgtcggt gtcgtgttta ctcattgctc tccctgattg ctttaatgaa
     6421 aaagtcatat aagttgccat gaacaatgtt tattcatcct ggcgatgata gttatcacca
     6481 ttattcttta tgggtataaa actatcggcg cagcatccta cccgttatcg ataaacgatg
     6541 caaaacatcc ccttacaatt ctgaagggga ttcatacaac tgacgaaaaa atgacaaata
     6601 cttttgctgg ttaacctgtg tgctgtccta gacttaatct ttaaaagatt gtgaggggca
     6661 tatgattaag gtgcatggcg tacccggatg gggcactaca atcagtgaga tgatgctaac
     6721 actggctgat attccttatc agttcgttga tgtcagtggt tttgaccacg agggaacttc
     6781 acgcgagtta ttgaaaaccc tgaacccgtt gtgtcagatt ccgacactgg cgctggaaaa
     6841 tgacgagatt atgacggaga cagcggcgat tgcattgatg gttctcgatc gtcgcccgga
     6901 ccttgcgcca ccggttgggc gcgccgaacg tcagcagttt caacggctat tggtctggct
     6961 ggttgctaat gtctatccaa cattcacttt cgccgattac cctgagcgtt gggctcctga
     7021 cgcacccgaa cagttaaaga ataatgttat tgaatatcgt aaatcgcttt atatctggct
     7081 gaattcacaa ttgaccgctg aaccttatgc gtttggtgag caactaacgc tggtggactg
     7141 ttatctttgc accatgcgca catgggggcc ggggcatgaa tggtttcacg ataatgcgca
     7201 gaatatcagc tc
//
LOCUS       DAZFLD010000210         7205 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.211, whole genome shotgun sequence.
ACCESSION   DAZFLD010000210 DAZFLD010000000
VERSION     DAZFLD010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7205
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.211"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            394..1257
                     /locus_tag="ACHKBL_004075"
     CDS             394..1257
                     /locus_tag="ACHKBL_004075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000897941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virB8 family protein"
                     /protein_id="HFO5820870.1"
                     /translation="MLFKSNKIKKETEQERLLHYGDGKLSGKSDEIGREIRAYTMAAR
                     WFEKRVAEDYRKKARNSRILAGLFGVIAIVAVIAVANLAPLKTVEPFVIRVDRNSGYM
                     DVVRPGWSKEDTKDISDDKHFISLYILSRETYNWASQKSNFAIVRQLSYSDVFSEYRN
                     FQLSGKGYVSTLNQSRQISVDIDSIVPLPVSTDKKLGERDDIKTYQVRFRQSLLDAEG
                     RPVPETGQILKLDENGKPIVKPKVVFWTAIISFDYLNTADTEGEAWLNPKGFGVLAYT
                     KTEEIRNEENN"
     gene            1241..2053
                     /locus_tag="ACHKBL_004076"
     CDS             1241..2053
                     /locus_tag="ACHKBL_004076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbG/VirB9 family P-type conjugative transfer
                     protein"
                     /protein_id="HFO5820871.1"
                     /translation="MRKIIKYIVPVCLIFSVTGFVHAEIAGKGSVSDKRIQTALYSPD
                     NVFRIYAMKDRTTMIQLEQGETINTDAGAMNLGKPGTEGAPEWLLGANKAGSLIMIKP
                     SRYAEDPRTNLIVSTNRRTYLFELHLTQSPALATYLFRFNYPKPPEVGKNPFVGRNIN
                     ENPCDGIINKKYQKRGDMIISPYQVWDNGTFTCFRFPTNAPRPVIYEVLPDGTETMVN
                     ARPVNDILVVHGVSREFRIRLNRLVLAVRSKVENTGWYNYKGTTTGEVREVK"
     gene            2053..3408
                     /locus_tag="ACHKBL_004077"
     CDS             2053..3408
                     /locus_tag="ACHKBL_004077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbI/VirB10 family protein"
                     /protein_id="HFO5820872.1"
                     /translation="MTENVVTPPENETKKETSAADENKPQGQGVGAFSLKPAGRKKPV
                     NWKIVGVIIGGIVIVLLFTIIFAAIVINNRADPEPEIDETGIKAEVVTRSIGVIDDPM
                     GAFMEAHLPQDRDKTESDSRTVQSSGKQESNGPVSDKKNTQTSGGQSTVNPVTPVTDI
                     RVPETLFSPVARFDSSGISQGVGGSSDGVTSSSGYDVSAEEGRLREFANADPAEIVNR
                     ALQSQADTVNGGISSDMRSNILGDLSSSRQYATAKAYKSPPRKYLLKRRTNFQCVLYT
                     AVKTDHSGFVGCRLTKPIFSADGSVILAEAGAELNGEQKIEVKQGQSTVFTSWSELET
                     TAGTEGGIRADLNGLGTGPMGSSGTDAYIDNHYGQRFGGAVMLSFIQDAIATAANAAQ
                     KNKSTYSFDNSEDNAESMAEKALENSINIPPTGYVLPGTVINVIVAQDIDFSSVYTVK
                     Y"
     gene            3619..4668
                     /gene="virB11"
                     /locus_tag="ACHKBL_004078"
     CDS             3619..4668
                     /gene="virB11"
                     /locus_tag="ACHKBL_004078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001090934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043684 - type IV secretion system
                     complex [Evidence IEA];
                     GO_process: GO:0044097 - secretion by the type IV
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-type DNA transfer ATPase VirB11"
                     /protein_id="HFO5820873.1"
                     /translation="MNTEFDLDKSELVLGWLDQSGVTERLSRQGVTEVAINRPFEIWT
                     ETSKDGWQREDAPWLTLNMCEHLAETLAVYNNRILGVKSPIKTVELPGGERGQIVIPP
                     ACEKSTVSMTFRKPSLQRFSLENYDSSGRFDNANRVRGRSKELLDWQKELKRLNAARE
                     YVEFFRLAVRHKLNIIIFGGTGSGKTTFAKAIVDVYPSNRRLLTIEELNEMKLPYHLN
                     HVHLLYGDYVTPKDLVACCMRMKGDHILLAELTGDETWDFMELLNTGHPGTVTTAHAN
                     SSLHGYARISGLIKQSAVGAGLDISYIDRRVRTSFDVVMYMEKTEILDITYEPEVQLA
                     LLNGASMESALSFAS"
     gene            4680..5042
                     /locus_tag="ACHKBL_004079"
     CDS             4680..5042
                     /locus_tag="ACHKBL_004079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5820874.1"
                     /translation="MEKLKLFIVIIALCCLFFVIGLYAGGWMWLELSGVDSSPSLMTL
                     ISQGGLPALNDRARMLLPWAWCVTVAITFFPAGVSLLAMTHGADGNRKDLHGNARFAG
                     KRELRRFWYEGPEKEDRN"
     gene            5042..6670
                     /locus_tag="ACHKBL_004080"
     CDS             5042..6670
                     /locus_tag="ACHKBL_004080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HFO5820875.1"
                     /translation="MGKQKIPDGWVLENGKLIQKRVTHYPPIIFGKDPYSNNLLVSYG
                     QTYLMLAAPPGSGKGVGIVIPNLLLYPHSVVVNDCKFENWDLTAGFRKACGQKVVRFS
                     PERLETHHWNPLKVINQDPLYRLGDVRTLASSLYVPDNMKNASWFSKAADVFTAIVLY
                     LIETPSLPLSLPQCYEIASLGTRIGEWAQRVIQARVGTPDALSEETVRELGVIISESK
                     GREFSTLMSFVTGRLSLYGEKLVALALTESDNEEENIDFSKLREEPTSIYFCVTEGAL
                     QKFGPLMNLFFSQAIRENSKILPEQGGNLEDGSLRLKYQVLFLEDEFAVMGRIKVMET
                     APALSRGAGLRYIIVFQNKSQVCSDDCYGPAGGKAVMDPFHIEVVFAPGDISTAEEYS
                     KRLGTYTERVASSSRNISEGQRRSRGRNVTLHARPLMLPQEVNELPYPEELVFMQPTK
                     WNPALKGRVKKIMWYKEPELTARVDPVRYPVPDIPAGNISKLKGLVVPISQYITDAVK
                     ISSPEGDYLNEEQKKRLNIPQVESERESYEDNDY"
     gene            6695..7006
                     /locus_tag="ACHKBL_004081"
     CDS             6695..7006
                     /locus_tag="ACHKBL_004081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000877799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbM/KikA/MpfK family conjugal transfer protein"
                     /protein_id="HFO5820876.1"
                     /translation="MKYSVIAVIILFLLSSKSYAEEVSIDSGDPCTVFMCMAGKVYGE
                     NSSECKGPTKKFFNIIKKKKGRIRWSKTFDARKAFLMNCPGADPAHVSKIMSKFGRKL
                     F"
BASE COUNT         2205 a         1189 c         1773 g         2038 t
ORIGIN      
        1 gaaattccaa attatgctgc atctattatg ggaggcgctc catctggtgg ggtagggagt
       61 atgtccaggc ttttaggaaa agcaactggt tttggtgctg caagtcacat gagtaaggca
      121 ttaggaagta aattagttga tcgttttcat ggtagaggga aaggtcgtat ttcctagtaa
      181 tttttctatc tctgcttata atccattttc tcgtcttctt tacaaaaact tcatttataa
      241 aaaggcttat catgaaaaat atattcgcta tatgtgcggc gctttgtgtt gtgctgggat
      301 gcatatcatg tagttcaccg ccagagttgt cccaaccatc tggtgaatgg attgatgtaa
      361 atccagagtc tgtgctgtta ccgggagaaa aaaatgttat ttaaaagtaa taagataaaa
      421 aaggaaactg aacaggaacg tctcttgcat tatggtgacg gcaaattatc aggtaagagt
      481 gatgaaattg ggagggaaat acgggcttac acaatggctg cccgttggtt tgaaaaaagg
      541 gtggctgaag attacagaaa gaaagctaga aatagtcgta ttttggctgg attgtttggt
      601 gttatagcta ttgttgctgt aattgcagtt gcaaatttag ctccacttaa aacagtagag
      661 ccttttgtta tccgagtaga tcgcaatagt gggtatatgg atgttgttcg tcccggatgg
      721 agtaaggaag atacaaaaga tatttctgat gataagcatt ttatatcatt atatatttta
      781 tctagagaaa cgtataactg ggctagtcaa aaatctaatt ttgcaatagt aaggcagttg
      841 tcttattccg atgtgttttc tgaatatcgt aattttcagt tgtcaggtaa agggtacgtc
      901 tcaacattaa atcagtctcg tcagatttct gttgatattg attcaatagt tcctttacct
      961 gtcagtactg ataaaaaatt aggtgagagg gatgacatca aaacttatca ggttcgtttc
     1021 agacaatcac tgcttgatgc agaagggcgg cctgttccag agacaggaca gatactgaag
     1081 ttggatgaaa acgggaaacc aatagttaag cccaaagtgg ttttctggac agcaataata
     1141 tcttttgatt atttaaatac ggctgatacg gaaggtgaag cctggttaaa tcctaagggc
     1201 tttggtgtgc ttgcatatac aaaaacagag gagatcagga atgaggaaaa taattaaata
     1261 tatagttcct gtatgtctta tattttcagt tactggtttt gttcatgcgg aaatagccgg
     1321 aaaaggttca gtcagcgaca aaaggatcca gacggcattg tatagtcctg ataacgtttt
     1381 cagaatttac gccatgaagg acagaacaac aatgatacaa ctggaacaag gggagacaat
     1441 aaatactgat gcgggtgcaa tgaatctggg gaaaccagga acagaggggg cacctgaatg
     1501 gttactcggt gctaataagg ccggaagttt aataatgata aaaccaagtc gatatgcgga
     1561 ggatcccaga acaaacttaa ttgtaagtac gaatcgccgg acatatttat ttgaattaca
     1621 tctgacacaa tcaccggctt tagcaacata tctgttcagg ttcaattatc caaaaccgcc
     1681 agaagttggt aaaaatccat ttgttgggag gaatataaac gaaaacccgt gtgatggtat
     1741 tattaataaa aaataccaaa aacgtggtga tatgattatt tcgccttatc aggtatggga
     1801 taatggtaca tttacctgtt ttcgttttcc gacaaatgcg ccacgtccag ttatttatga
     1861 agttctacct gatggtacag aaactatggt taatgcgcgt cctgtaaacg atattttggt
     1921 tgtgcatggt gttagtcgtg agttcagaat tcgtcttaac agactggttc ttgctgtacg
     1981 ttctaaagta gaaaatacag gatggtacaa ttataaggga acaactacag gtgaagtcag
     2041 ggaggtcaaa taatgactga aaatgtagtg acaccaccag agaatgaaac gaaaaaggaa
     2101 actagcgctg ctgatgaaaa taagcctcaa ggtcaaggtg taggcgcatt ttctttaaaa
     2161 ccagctggtc gtaagaagcc tgttaactgg aaaatagttg gtgtaattat tggcggtatt
     2221 gttattgttc ttttgtttac aattatattt gccgcaatag ttattaataa tcgtgcagat
     2281 ccagaaccag aaatagacga aacagggatt aaagcggagg tcgtaactcg ctctataggg
     2341 gtaattgatg atccaatggg agcgtttatg gaggctcatt taccacaaga cagagacaaa
     2401 acggaatctg acagtagaac cgtccagtcg tcaggaaagc aggagagtaa cggacctgtt
     2461 tcagacaaaa aaaatactca aacttcaggg ggccagtcta cggtcaaccc ggttacaccg
     2521 gttacggata tcagagtacc agaaacgttg ttttcgcctg ttgcacgatt tgattcttcc
     2581 ggcattagcc aaggagttgg tggtagtagt gacggtgtaa catcatcttc tggttatgat
     2641 gtttcggcag aagagggtcg tctacgagag tttgccaatg cagatccggc agagatagtt
     2701 aaccgtgctt tacagagtca ggcagatact gtgaatgggg gaatatcttc ggatatgaga
     2761 tctaacatcc tgggggatct ctcatcatcc cgccagtatg caacagctaa agcttataaa
     2821 tcgcctccac gtaagtatct cctgaaaagg agaacaaatt ttcagtgcgt actttatacg
     2881 gcggtgaaaa cggatcactc tggattcgtt ggttgtcggt taacaaaacc cattttttca
     2941 gcggacggta gtgttatttt ggctgaagca ggtgctgaat tgaatgggga acaaaaaatt
     3001 gaagtaaaac aagggcaatc tacggtgttc acttcatgga gtgagcttga aactactgcc
     3061 gggacagaag ggggtatacg agcagattta aatggattag ggacggggcc gatgggaagc
     3121 agtggcacgg atgcatacat tgataatcat tatgggcaga ggtttggtgg agccgtgatg
     3181 ctatcgttta ttcaggacgc gatagcaaca gctgcaaacg cggcacaaaa aaataaatca
     3241 acgtatagtt ttgataacag tgaggataat gcggaaagta tggctgaaaa agcactggaa
     3301 aattctataa atataccacc tacaggatat gtattacctg gaacagttat taatgtaata
     3361 gttgctcagg atattgattt ttcgtcagtc tatacagtta aatattaatg atatatagcc
     3421 gccttaacgg gtggctatat attaaagtaa ttgcttgaag gaggttggat gggatttttt
     3481 aaatgggtgt gtatttattt ttatcgtatc ccaataaatt tacggtggtc aatcataatg
     3541 atcgttgctg catttatcat ttcattaatg agatagaaaa gtaactcata aaagtgtaaa
     3601 taaaaagggc gtctattaat gaataccgaa tttgacctcg ataagtcaga acttgttttg
     3661 gggtggctgg atcagtctgg tgttacagaa cgtctgtccc gtcagggagt gacggaggta
     3721 gcgattaaca gaccttttga gatctggacc gagacatcaa aagatggctg gcaaagggaa
     3781 gatgcaccgt ggttaacgct gaatatgtgt gaacacctgg ctgaaacact ggctgtttat
     3841 aataaccgta tattaggtgt taagtcaccg ataaaaacag tggaactacc aggaggcgaa
     3901 cgaggacaaa ttgttattcc tcccgcctgt gagaaatcta ctgtgtcgat gacgtttcgt
     3961 aaaccgtctc ttcaacgttt ctctctcgag aattatgact ccagtggacg tttcgataat
     4021 gccaatcgtg taagaggaag aagtaaagag ttactggact ggcaaaagga attaaaacgt
     4081 cttaatgctg ctcgtgaata tgttgaattt ttccgtctgg ctgtgagaca taaactaaat
     4141 attattattt ttggtggaac cggttcggga aaaacaactt ttgctaaagc aattgttgat
     4201 gtttacccgt caaatcgtcg gttactgact attgaagaat tgaatgaaat gaagctgcct
     4261 tatcatctta atcacgtaca tttattgtat ggagattatg tgacaccaaa agacctggta
     4321 gcgtgttgta tgcgcatgaa gggggatcat attcttctgg cagaactgac aggcgatgaa
     4381 acttgggatt ttatggaact gctgaatacc gggcatccag gaacggttac gacagcgcat
     4441 gctaatagca gtttacatgg ttatgctcgt atctctggac taataaagca gtctgcggtt
     4501 ggtgccgggc tggatatctc ttatatcgat cgacgtgtca gaacgtcttt cgatgttgtg
     4561 atgtatatgg agaaaacaga aatcttggat attacgtatg agccagaggt gcaactggca
     4621 ttactgaatg gtgccagcat ggaaagtgct ctttcttttg catcgtgagg tagtgctgta
     4681 tggaaaaact caaactattt atcgtgataa tagctctttg ttgtttgttt tttgttattg
     4741 ggctttacgc tggaggatgg atgtggcttg aattatcagg tgttgactcc tctccatctt
     4801 tgatgaccct gataagtcag ggaggactgc cagcactgaa tgatcgggcc agaatgttac
     4861 ttccctgggc gtggtgtgtg acagttgcaa ttaccttttt cccggcaggt gtttctcttt
     4921 tggccatgac tcatggagcg gacggcaaca gaaaggacct acatggtaat gctaggtttg
     4981 ccggaaaacg ggaattgcgt cgtttctggt atgaagggcc agagaaggag gacagaaatt
     5041 aatgggaaaa caaaaaatac cagacggatg ggtgctggaa aatggaaagt taatccaaaa
     5101 aagagtaact cattatccgc caattatttt tggtaaagat ccgtacagta ataatttact
     5161 tgtatcctat ggacagacct atctgatgtt ggccgctccg ccgggaagtg gtaaaggggt
     5221 ggggattgtg atccctaatc tactattgta tccgcattct gttgtagtga atgactgtaa
     5281 atttgaaaac tgggatttga cagcgggttt cagaaaagca tgtggtcaga aagtcgtgcg
     5341 tttttcgcca gagcgactgg aaacccatca ctggaatccg ttaaaggtaa taaatcagga
     5401 tccgctgtat cgcttgggag atgttcgaac actagcctca tctctgtatg tacctgataa
     5461 catgaagaat gccagttggt tttctaaagc cgcagatgtg tttaccgcga tagttttata
     5521 cctgatcgaa acaccatcgt tgccattgag tttaccgcaa tgttacgaaa ttgcatcgtt
     5581 gggaactcgt attggagaat gggcgcagcg tgttattcag gctcgtgtag gaacgccaga
     5641 tgcactgtca gaagaaaccg tccgagagtt gggggtgatt atttccgagt cgaaaggcag
     5701 ggaattttca acgttgatgt cattcgtcac gggacgtcta agcttgtacg gcgaaaaact
     5761 ggtggcgctg gcgctgacgg agagtgataa cgaggaagaa aatattgact tcagcaagtt
     5821 aagggaagaa ccaaccagca tttatttttg tgttaccgag ggagctttac agaaatttgg
     5881 tccactaatg aatttgtttt tttctcaggc aataagggaa aacagtaaaa tactaccgga
     5941 gcaaggggga aatctggagg acggttctct cagattgaaa tatcaggtgc tttttctgga
     6001 agatgaattt gctgtgatgg gccgtatcaa ggttatggaa acagccccgg cactaagcag
     6061 gggggcagga ttacgctata taattgtttt tcagaataaa agtcaggttt gctctgatga
     6121 ctgttatgga cccgcagggg ggaaagctgt aatggatcca ttccatattg aggttgtatt
     6181 tgcccccgga gatatcagta cagcggagga gtacagtaaa cgactgggaa cgtacacaga
     6241 acgagttgcg tccagtagca ggaatatatc ggaaggacag cgtcgttctc gaggacgtaa
     6301 tgttacactg catgctaggc ctttaatgtt gccacaggag gttaatgaac ttccatatcc
     6361 tgaagaactg gtgtttatgc agccaacaaa atggaatccc gccttgaaag gccgggtgaa
     6421 aaaaataatg tggtacaagg aacctgaatt aacagcaaga gtggatcctg ttcgttatcc
     6481 cgtgcctgat ataccagccg ggaatatatc gaaacttaaa ggacttgttg taccaataag
     6541 tcagtatatt acggatgcag ttaagatatc ttcaccagaa ggtgattatt taaatgagga
     6601 gcagaaaaaa cgattaaaca tcccacaagt ggaatcagaa agggaatctt atgaagataa
     6661 tgattactga atttttaata aaaagggggt taatatgaaa tattctgtca tagcggtgat
     6721 tattcttttc ctgttatcca gtaaaagcta tgctgaagag gtaagtatag attcggggga
     6781 tccctgtact gtctttatgt gtatggctgg aaaagtttat ggggaaaatt catctgaatg
     6841 taagggacca acaaaaaaat tctttaatat tataaaaaag aagaaagggc gtattcggtg
     6901 gagtaaaact tttgatgcga gaaaagcatt tttaatgaat tgccctgggg cagatcctgc
     6961 tcacgtcagt aaaattatga gtaagtttgg aagaaaactt ttttgaataa aataatcaaa
     7021 ggctttagaa ctaaactaga ggcgtttgaa ctattctccg cagatgacat atgtttatcc
     7081 tagatttgcg gagaggaata actatttcca catcaactat gggaatgaaa tatatggata
     7141 aatcagatga ggaaaaaata ggttatattc tggatgaaca tgagcggata ttcagaaaac
     7201 aggct
//
LOCUS       DAZFLD010000211         7096 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.212, whole genome shotgun sequence.
ACCESSION   DAZFLD010000211 DAZFLD010000000
VERSION     DAZFLD010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7096
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.212"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..579
                     /locus_tag="ACHKBL_004082"
     CDS             <1..579
                     /locus_tag="ACHKBL_004082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFO5820877.1"
                     /translation="IHLIKHFMRNEHKAVEEVIDSPLSEVANLIKVLQSCQGKVVFIG
                     VGKSGIIARKLAATFASTGTPSFFVHGTEAVHGDLGMVAKDDVVILISNSGETTEILA
                     TLPSLKKMGNYLISFTRSHRSSLAISCDLSVEIPVKSEADNLGLAPSCSSTVVLVVGD
                     AVALALSELKKFTRADFGLYHPGGALGIKANS"
     gene            650..1597
                     /locus_tag="ACHKBL_004083"
     CDS             650..1597
                     /locus_tag="ACHKBL_004083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001361319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HFO5820878.1"
                     /translation="MKNVLVTVPSFSARCVSASKLLRENHFNLIIKNNVEHLVKSEST
                     VLRESICAVIAGKDCYQADTLSRLPGVKIISRFGTGIDNIDLHTARQSGIVVNNAVGI
                     NSNAVAEFIIGLIFASMRNIPGSYHAMQNGYWGESHGCELQGKRIGLLGYGNIGKTLA
                     KRLSGFDVELLAFDKQPDYQVADKAGVQFVSIEDIFMQSHVIIVLLPFSSELENFISH
                     KYLSMMRNGALIINAARGKLLDEGALLQVIEERNVFAALDVFSSEPLAQFSPLLHAKN
                     IITTPHIAAATVESYQQTGIHVAQSIIDYFAGREIKNVL"
     gene            complement(1899..3275)
                     /locus_tag="ACHKBL_004084"
     CDS             complement(1899..3275)
                     /locus_tag="ACHKBL_004084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VasL domain-containing protein"
                     /protein_id="HFO5820879.1"
                     /translation="MASNANFISQFVMGGDPCTYKESGELQAEMSKLTHPARPDVDWR
                     QVEKLCLALFRQNGVELQTLVCYVLAITRRQGLAGMADGLGSLDILLQRWADFWPVQV
                     HSRISLLSWVTEKMQQALRTLDIQYQDLPQIYRCVQHLSAIETTLQQCELWHMTKLDV
                     LSGQFRNAALRLERLAPQGAETIITPPELPRREMNQPKKSEESPQPVFATRSVQQNDK
                     DASPPVPSPEISRQRTWPIFMAGMVVMAGLGGTGLWGWSQLNQPDALIQRIQLSVMPL
                     PQSLESGELAKLDVKDKALLAQDRTIAASQMQLEQLNKLPARWPLEQGYRQLRQLDAL
                     WPDNPQVRALNAQWRKQRELSALSAEALNGYAQAQSQLQRLSAQLDALDERKGRYLTG
                     SELKTAVYGIRQSLKEPPLEELLRQLEEQKQTGEVSPTLLTQIDTRLNQLLNRYVILL
                     DTKVEQSQ"
     gene            complement(3275..3703)
                     /gene="tssE"
                     /locus_tag="ACHKBL_004085"
     CDS             complement(3275..3703)
                     /gene="tssE"
                     /locus_tag="ACHKBL_004085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000106970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HFO5820880.1"
                     /translation="MATPSLYEMLTFSFSGELPLEQVSERDQLILSVMDNMQRIINCR
                     AGTLAHLPDYGLPDLSLIHQGMAAGIHSLMRQIEETLLRYEPRLNQIQVELLPQPGPG
                     HLNYLIHAQLPDTGWIRFDGVFSPEGRIVLRHLKQQERAY"
     gene            complement(3707..4243)
                     /gene="tssJ"
                     /locus_tag="ACHKBL_004086"
     CDS             complement(3707..4243)
                     /gene="tssJ"
                     /locus_tag="ACHKBL_004086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000484007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system lipoprotein TssJ"
                     /protein_id="HFO5820881.1"
                     /translation="MFPIRFKRPALLCMAMLTVVLSGCGLIQKVVDESKSVASAVFYK
                     QIKILHLDFFSRSALNTDAEDTPLSTMVHVWQLKTREDFDKADYDTLFMQEEKTLEKD
                     VLAKHTVWVKPEGTASLNVPLDKETQFVAIIGQFYHPDEKSDSWRLVIKRDELEADKP
                     RSIELMRSDLRLLPLKDK"
     gene            complement(4224..5303)
                     /gene="tssG"
                     /locus_tag="ACHKBL_004087"
     CDS             complement(4224..5303)
                     /gene="tssG"
                     /locus_tag="ACHKBL_004087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000373314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HFO5820882.1"
                     /translation="MDRASQPARTGLTDRLAPDIARINFYRFCQLLEQSQQNAPLGST
                     DSPATDAVRFRPHPGMGFPVSELKNVERDVGNPDAPPTVRTTFLGLYGVDSPLPTAYL
                     DYITQRHDGHDAVMAFLDIFNHRFITQYYRIWRKYNYPASFEAGAVDDISRCLLGLIG
                     LGIPGSENHIATPVSRFLALLSVMRLPTRTAEGVTALVGLLAPLTKATVVPHDPQPVI
                     LPAPAGLSKNSRISLKTRTLLGRTGTDVNSQLLLKLYTEDAAEARGWLPGGQLHSDLL
                     VLLRVYLGWRCQARLQLTLPVSLLPAARLGKQRVQISRTGILRASFAAPATGTVTVSL
                     GRYQGLIPAFSIRNRESMTHVSYSF"
     gene            complement(5267..7027)
                     /gene="tssF"
                     /locus_tag="ACHKBL_004088"
     CDS             complement(5267..7027)
                     /gene="tssF"
                     /locus_tag="ACHKBL_004088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HFO5820883.1"
                     /translation="MDDLTQRYYEAEMRYLREAGKEFAQAYPDRAAMLNLDKPGARDP
                     YVERLFEGFAFLMGRLREKLDDDLPELTEGLVSLLWPHYLRTIPSLSVVELSTDHRQM
                     KQSETLSNFQVLSRPVGERRTRCVYSATRDITLHPLALPDVSLQYEPDGRSVIRLRFE
                     CGPLVGDWSQIDLSRLPLYLNADSPVACALHRALTLGTQQFWLRLPGQERRVLDAHFS
                     PMGFDDNDRLWPKGESAFSGYQLLLEYFTFREKFMFVALNGLENVIWPEGMSGFEIDV
                     VLSENWPHDLPFDSDNIRLHCVPVINLFPLEADPLHLSPLENEFLLRPMRIQDGHTEI
                     YSVDNIISSRHTGSQAYVPFSSFRHRGGMLRHDAPERYYHTRVKRGPSGLHDTWLILG
                     GDAFDSDRMLEDETLSLSLTGTNGQLPRKALQSTLLDTPVHASQNVLRVRNLCAPTQP
                     CYPPARDRFHWRVLSHLGSNFLSMMDNAEILRGTLALYDWTESEMNRRRLSAIVDVQH
                     SLIQRFERGFLLRGVDIQVTLDSTGFAGEGDITLFGELLHRFFALYADIHLFTQLTLI
                     LQPTGKCLQWTEHHSQRVPG"
BASE COUNT         1671 a         1854 c         1866 g         1705 t
ORIGIN      
        1 atccatctca ttaaacattt tatgcgtaac gaacataaag cagttgaaga agttattgat
       61 tccccccttt cggaggtcgc aaatttaatt aaagtattac agtcttgtca ggggaaagtt
      121 gtttttattg gtgtcggtaa gtccggtatt attgcccgaa aactcgccgc aacatttgcc
      181 agcactggaa ctccctcgtt ttttgttcac ggtactgaag ccgtacacgg cgatcttggg
      241 atggtggcga aagatgatgt tgttattctt atttcaaaca gtggtgagac gacagaaatc
      301 ctggcaacac tacctagtct gaaaaaaatg ggtaattatt tgatctcctt tacccgcagt
      361 catcgctcat cgcttgcgat aagttgtgat ttatccgttg aaattcccgt aaaaagtgag
      421 gcggataatt taggtttagc gccgtcatgc tcatcaacag tcgttctggt tgttggtgat
      481 gcggtggcgc ttgcgctctc cgaattgaaa aagtttactc gtgctgattt cggcttatat
      541 catccaggag gtgcgctcgg tattaaagca aactcataat tttagaatat aaaacagtaa
      601 ccggtcacgc catcttttgg ggtggcgttc attgatgtga gtgtcagcta tgaaaaacgt
      661 tctggtcacc gttcccagct tttcagcgcg ttgtgtttct gccagcaagc tgctaagaga
      721 aaatcatttt aaccttatta tcaagaataa tgtggagcat ctggttaaat cagaatccac
      781 agtattacga gaatctattt gcgcagtcat tgccggaaaa gattgttatc aagcggatac
      841 gttatcgcga ctaccaggag tgaaaataat atcgagattc gggacaggga ttgataatat
      901 tgatcttcat acggctcgac aatcaggtat tgtggtcaat aatgctgttg ggattaattc
      961 aaatgcagtc gcggaattta tcatcggtct tatttttgcg agcatgagaa atatccccgg
     1021 tagctatcat gcaatgcaaa atggttattg gggtgagtcg catggctgcg agttacaagg
     1081 taaacgtatt ggcctgcttg gctacggcaa tattggcaaa acactggcga aaaggttatc
     1141 tggttttgat gttgagcttt tagcttttga caaacaaccc gactatcagg ttgctgacaa
     1201 agctggagtt cagtttgtat caattgaaga tatctttatg cagtcgcatg tcatcattgt
     1261 tcttttgcct ttttcttctg agctggagaa cttcattagt cataaatatt tgtcgatgat
     1321 gcgcaatggt gctctgatca tcaatgccgc aaggggaaag ttgctggatg agggcgcttt
     1381 acttcaggtg attgaagaac gaaatgtgtt tgcggcgctg gatgtgttta gctctgaacc
     1441 gttggcgcaa tttagcccac tgctacatgc caaaaatatc attacaacgc cgcatatcgc
     1501 ggccgccacc gttgagtcat accagcaaac cgggatacac gttgcccagt cgataatcga
     1561 ttacttcgca ggaagggaga taaaaaacgt gttgtgagtt cagcgaacaa agattttaaa
     1621 tacaaaaaaa ccgcaatttg tagaacacat tgcggtttgt cagcattcta ataaattggt
     1681 tgcgggggcc acagtcaaga ggttaatcca atcttttgaa acttaaggta ttcactgttc
     1741 ccgaacccaa gctatctacc gaaatataca ctcattatct gatgtgaatc aacgagtaga
     1801 tcgaatggcc tgttcctctc tttttttgta cacagagact ccattttaaa aaatggcaag
     1861 gtcacatatc ccttattggt acataaatcc cccgatgttt actgactttg ttccaccttc
     1921 gtatccagta aaatgacata gcgattcaac aactgattta accgggtatc aatttgcgtc
     1981 aacagcgttg gcgaaacctc tccggtctgt ttttgctctt ccagttgccg aagcagttct
     2041 tccagcggcg gctcctttaa cgactgccgg atgccgtaca ccgccgtttt tagttccgaa
     2101 ccggtcagat atctcccctt acgttcatcc agtgcatcca gctgcgccga caggcgctgt
     2161 agctggctct gcgcctgagc atagccattc agtgcctcag cagacagggc gctcagctcc
     2221 cgctgtttgc gccactgcgc gttcagcgcc ctgacctgag gattatccgg ccacagggca
     2281 tcaagctggc gtagctggcg atatccctgc tccagtggcc agcgggcagg caatttgttt
     2341 aactgctcca gttgcatctg acttgccgca attgttctgt cctgagccag cagcgcctta
     2401 tcctttacat ccagctttgc cagttcgccg ctctccagcg actgcggcaa cggcatgaca
     2461 gacagttgta ttcgctggat tagcgcgtcc ggctgattaa gctgcgacca gccccataat
     2521 cctgttccgc cgagaccggc catcacaacc attccggcca taaatatcgg ccatgtccgc
     2581 tgccgggaga tttcagggga tggcacgggt ggactggcat ccttatcgtt ttgctgaacg
     2641 gatctggttg caaaaaccgg ctgtggactt tcctctgact ttttcggttg attcatttcc
     2701 cggcggggta attcaggggg agtgataatg gtttccgctc cctgaggcgc cagccgttcc
     2761 agacgcagag ccgcattgcg aaactgcccg gacaatacgt ccagcttcgt catatgccac
     2821 agttcacact gttgcagcgt ggtttcgatg gcggaaagat gctgtacaca acggtagatc
     2881 tgcggcagat cctgatactg aatatccagc gttctcagtg cctgctgcat tttttctgtg
     2941 acccagctga gcagtgatat tctggaatgt acctgtaccg gccagaagtc agcccagcgc
     3001 tggagcagta tatccagtga accgagtccg tctgccatcc ctgccagccc ctgccgtctg
     3061 gttattgcca gcacgtaaca gaccagcgtc tgtagttcca cgccattttg ccggaacagc
     3121 gcgaggcaga gtttttccac ctgacgccag tccacatccg gtcgggccgg gtgagtcagt
     3181 ttactcattt cagcctgcag ttcaccggat tccttataag tacagggatc gccgcccatg
     3241 acgaactggc tgataaaatt cgcgttactt gccatcagta cgcccgctcc tgttgtttga
     3301 gatgacgcag aacaattcgt ccttccggag aaaatacgcc atcaaagcgt atccagccgg
     3361 tatcgggaag ctgcgcgtgg atcaggtaat taagatgccc cggaccgggc tggggcagta
     3421 actccacctg tatctgattc aggcgtggtt cataacgcag cagcgtttct tcaatctggc
     3481 gcatcaggct atgaataccc gcagccatcc cctgatggat caggctgaga tccggcagac
     3541 cgtaatccgg caaatgcgcc agtgtgcctg cccgacagtt gataatgcgt tgcatattgt
     3601 ccatcacgga aaggataagc tgatcccgct cactgacctg ttccagcggt aactcgccgc
     3661 tgaaactgaa cgtcagcatt tcatataagg aaggtgtcgc catcggttat ttatccttga
     3721 gaggcagcag tcgcaggtcg cttctcatca gttcaatcga gcgcggcttg tcggcctcca
     3781 gttcgtccct tttgatcacc agacgccagc tgtcgctttt ttcatccggg tgataaaact
     3841 gcccaataat ggcgacaaac tgcgtctctt tatccagcgg cacattcagg gatgccgtgc
     3901 cttcaggttt tacccagacg gtgtgttttg ccagtacgtc cttctccagc gtcttctctt
     3961 cctgcataaa cagggtgtca taatccgcct tatcaaaatc ttcgcgggtt ttcagttgcc
     4021 agacatgcac catcgtggaa agcggcgtat cttccgcatc cgtattcagg gcgctgcggg
     4081 agaagaaatc gagatgcagt attttgattt gtttgtagaa aacggctgag gccacgcttt
     4141 tcgattcatc caccactttc tgaatcagcc cgcagccact cagaacaacc gtcagcatcg
     4201 ccatacagag caacgccgga cgtttaaaac gaataggaaa catgagtcat actctccctg
     4261 ttacgaatgg aaaaagcggg tattaatccc tgataacgcc ccagactgac cgtcactgtt
     4321 ccggtcgcgg gcgcggcaaa ggaggcgcga aggatgccgg tacggctaat ctgtacacgc
     4381 tgttttccca gccgggcagc aggcaacagg ctgacgggca gcgtcagttg cagacgggcc
     4441 tgacagcgcc agcccagata aacgcgcagc agcaccagca aatcgctgtg cagttgcccg
     4501 ccgggcagcc agccacgtgc ttctgcggca tcttcggtat acagtttcag cagcaactgg
     4561 ctgttgacgt cggtaccggt acgccccagc agcgttcgcg ttttcaggct gatgcgtgaa
     4621 ttttttgaca gacccgcagg ggccgggaga attacaggct gcggatcgtg aggcaccacc
     4681 gtcgctttgg tcagcggggc aagcagccct accagtgccg tgacgccttc cgccgtgcgc
     4741 gtcggcaggc gcatcacgct gagcagggcc agaaaacggg ataccggcgt ggcgatatgg
     4801 ttctcgctgc cgggaatacc cagcccgatg agtcccagca gacaacggga aatgtcatcc
     4861 acggcacctg cttcaaacga cgccgggtag ttatatttgc gccagatgcg gtagtactga
     4921 gtaataaagc gatggttaaa aatatcgaga aacgccatca ctgcgtcatg tccgtcatga
     4981 cgctgggtaa tgtagtccag ataggcggtt ggcagcggcg aatccacgcc gtacaggccg
     5041 agaaaagtgg tgcgcaccgt ggggggcgcg tcgggattgc ccacgtcacg ttcaacgttt
     5101 tttaactcgc tgaccggaaa gcccatacct ggatgaggac gaaagcgcac cgcatcggtg
     5161 gcggggctgt cggtactgcc cagcggcgcg ttctgctggc tctgttccag caactggcaa
     5221 aaacggtaaa aattgatccg cgcaatatcc ggggccagcc ggtcggtcag cccggtacgc
     5281 gctggctgtg atgctctgtc cattgcaggc actttcctgt gggttgcaga attaaggtca
     5341 gttgagtgaa taagtggata tcggcataca gtgcaaagaa gcggtgcagc agttcgccaa
     5401 acagcgtgat atcaccctct ccggcaaagc cggtgctgtc cagcgtcacc tgaatatcca
     5461 cgccacgcag cagaaagcct ctttcaaaac gctgtatcag gctgtgctga acgtcgacaa
     5521 tcgccgacag acggcggcgg ttcatctcgc tttcggtcca gtcgtacagc gccagcgtgc
     5581 cgcgcagaat ttcggcgtta tccatcattg aaagaaagtt agaaccgagg tggctgagca
     5641 cccgccagtg gaagcggtca cgcgccgggg gataacaggg ctgcgtcggg gcgcacaggt
     5701 tgcgcacccg caggacgttc tgtgaggcat gaactggcgt atcaagcagc gtgctctgta
     5761 aagccttacg gggaagctgt ccgttagtgc cggtcaggct taaagagagc gtttcatcct
     5821 ccagcatccg gtcggaatca aacgcatcgc cgcccagaat cagccaggta tcatgcaggc
     5881 cggaagggcc gcgcttaacc cgcgtatggt aatagcgttc cggtgcatcg tggcgcagca
     5941 tcccgccccg gtgacgaaag ctggaaaacg ggacatacgc ctgactgccg gtatggcgcg
     6001 aagaaataat gttgtcgacg gaataaatct cggtatgccc gtcctgaata cgcatcgggc
     6061 gcagcagaaa ttcgttttcc agtggcgaaa ggtgtaacgg atcggcttcc agcgggaaca
     6121 ggttgatgac cggcacgcag tgcagacgga tattatcgct gtcaaacggc aggtcgtgag
     6181 gccagttctc gctcagcacc acgtcaatct caaagccgct catcccttcc ggccagatga
     6241 cgttctccag cccgttcagc gccacaaaca taaacttttc gcggaaggtg aaatactcca
     6301 gcagcaactg gtagccgctg aacgccgact cgccttttgg ccacagtcgg tcattgtcat
     6361 caaaacccat cggggaaaag tgcgcgtcca gcacacggcg ttcctgcccc ggcagacgca
     6421 gccagaactg ctgggtgcca agcgttaaag cccggtgcag cgcacaggcc accgggctgt
     6481 cggcattgag atagagcggc aaacgggata gatcgatctg tgaccagtca ccaaccagcg
     6541 ggccgcattc aaagcgcaga cggataaccg agcgcccgtc cggttcatat tgcaatgaca
     6601 cgtccggcag cgccagcgga tgcagcgtga tatcccgcgt agcactgtag acacagcggg
     6661 ttctgcgctc gccaacagga cgcgacaata cctgaaaatt actcagtgtt tcgctctgct
     6721 tcatctgccg gtgatctgtc gacagttcaa ccacggaaag tgacgggatg gttcgcaggt
     6781 agtgaggcca cagcaggctg accagcccct cggtcagttc gggcaggtcg tcatccagct
     6841 tttcacgcag gcgacccatt aaaaaagcga agccttcgaa caggcgctcc acatagggat
     6901 cgcgtgcgcc gggtttatca agattgagca tcgcggcgcg gtcggggtaa gcctgcgcaa
     6961 attctttccc cgcttcacgc aggtagcgca tttcggcttc gtagtagcgt tgggttagat
     7021 cgtccatcgg agggattatc ttcctttcat tgaaagtgaa tatcatgcag gtgtagaagt
     7081 aacatcatag ttaaat
//
LOCUS       DAZFLD010000212         7075 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.213, whole genome shotgun sequence.
ACCESSION   DAZFLD010000212 DAZFLD010000000
VERSION     DAZFLD010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7075
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.213"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..490
                     /locus_tag="ACHKBL_004089"
     CDS             <1..490
                     /locus_tag="ACHKBL_004089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tail tape measure protein"
                     /protein_id="HFO5820884.1"
                     /translation="RAGGLLSSVGSRMGLGRFSGFFRSAGGLAGRLGGGALWAGAMAA
                     PVLLDGSASATDKGEAVGSLAGSIAGGALGAAAGPVGIAIGSTVGSYLGNYLGGWLTE
                     AWQKLRGSDDESSGQAVQKASARVELVAPEGWQARSIDIDDTSGHGLDVNVWNGGNYG
                     LW"
     gene            477..1826
                     /locus_tag="ACHKBL_004090"
     CDS             477..1826
                     /locus_tag="ACHKBL_004090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA circularization protein"
                     /protein_id="HFO5820885.1"
                     /translation="MASGDGRGAFRGVPFLVWREQRERGGRNIVRREYPLRETGGADD
                     LGPKLAEFTFSVLVMGDDVQTQRNRLRDALRAPGAGELLHPDYGTLNVLINSFESRYN
                     AAEQGVVEFTINVTPVSDDTAPAVTQDTAAILEQKSTTALGKVFETLEAGWTVISDGM
                     HDVQAMTETISDKVSALENAVSGMGIVQDISAFTATFTALKGNATALLTAPSRMASSF
                     AGLFSALITLPSLPSLSSGGLNTRPGGSIGRTTSAVSQGMPQLYRTLSSLRYVLDEQD
                     NPQTLIGLTPAAQKNIRLIRAVMQSAAVVAQAQTVGKLLDQVLSQETLPDSDAAHRTW
                     PVWLESSVDLQRINRDLSEALERQVMTLSGQGYTATALTLRDARLALTEDLNTRGVQL
                     PGATVVTVRTTEPALVTLYRATGNSTGWQRFVRRNGIVDPLFIPGGHSVEVISEQQG"
     gene            1810..2934
                     /locus_tag="ACHKBL_004091"
     CDS             1810..2934
                     /locus_tag="ACHKBL_004091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein"
                     /protein_id="HFO5820886.1"
                     /translation="MSSRVELYLGGEIFSGWLTVSVRRSLEHLAGSFELGVMMPGVRL
                     PSSVRAGQSLELRIDGQPVITGWLDQVRQRISATRFQITLSGRDKTGDLVDCSAIHPG
                     SQWRNRTLEHIASDLCAPFGVTVRWQVNDATAARPFSTFTLENSETVADALTRAARHR
                     GVLVTSNAAGELVFTQAGSQRGDTLTLGENLLDLDHNVDHRLRHSEYRVRGHGRGGGH
                     AGDALTAGTLAAPVGTVTDSAIHRYRPKIVLADHAVDADGARQRAVREMRRAVARSVR
                     LTATVRHWFRENGQLWDINLLTAVTAPRTGVEERDLLVCQVEFSLDANHGETTRLILA
                     PRDGFIVPAEPGNSGSGNAGDVDAFVRAQMKKQGIKFNDE"
     gene            2924..3538
                     /locus_tag="ACHKBL_004092"
     CDS             2924..3538
                     /locus_tag="ACHKBL_004092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HFO5820887.1"
                     /translation="MMNDEVISRLLAPVMRGVRLLFGRGVLTGTTDTLKIQNVQITGM
                     DGETFDDVERPQQYGQISVPLPGAEVFLACAGGQRDQAVVLVVEDRRSRPTGLTAGDT
                     GVYHHEGHRIRLTKNGRIIVTCKTLEIYADEGVQVDTPEAHFTGNVTVDKNLHVKGNV
                     SIDGTGRSQGTFTMSEAVIAGITYSGHVHHDNGEGSKTGGPENG"
     gene            3531..3968
                     /locus_tag="ACHKBL_004093"
     CDS             3531..3968
                     /locus_tag="ACHKBL_004093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage GP46 family protein"
                     /protein_id="HFO5820888.1"
                     /translation="MADIAVVWDQGCGSLQLNGADLLTDNSLLTAVIISLFTDRRALD
                     SDEIPDGTRDRRGWWGDSFRERPVGSRLWLLSREKTLSSVVSRAQAYADEALAWLHKS
                     GAATSVVCHAMRVGHARLSLSVKITLPDGSRHPMIFYADMKGE"
     gene            3968..5050
                     /locus_tag="ACHKBL_004094"
     CDS             3968..5050
                     /locus_tag="ACHKBL_004094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313014.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HFO5820889.1"
                     /translation="MPYQPLPLAQLITQTQQDISQRLPGSQPGVNETTLNAIAYALAG
                     LSAQEHEHLAWISRQIIPTEADEAELLKHCAFWGVIRKPASRADGPVQLMLTTDAGIT
                     EGVLLQRSDGVVYRITGSATGKAGTLNVNVEAESAGRAGNTPTGTRLSFITPQAGINQ
                     TATVTGTGLTGGADVETVPELLSRLVFRVQNPPSGGTQYDFERWAREVPGVTRAWCKP
                     EWPEAGSVGVTFVQDNNPDIFPGEGDVKRVADYIRSHDDPATGQPVGQPLGPTISVFK
                     LTNKPVAFEIRIVPKTPENQAAVKQALTDLLYNESRPGGLVLPSSFWRAVAGVKGLED
                     FEVRSPLKSVMAGDTELLTVGEITWL"
     gene            5041..5601
                     /locus_tag="ACHKBL_004095"
     CDS             5041..5601
                     /locus_tag="ACHKBL_004095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative phage tail protein"
                     /protein_id="HFO5820890.1"
                     /translation="MAVTLTPHQRALLQLLPDGLAWDKRPSSVLAALCLGLSHSTERV
                     SWTGNQMLAERFPDSSRLLLEDWERYLGLPECDMTGATIQERQRYAGNKYRMKPSLNR
                     EFYIRFAAKFGYEIDIQPSPDSQWVSIVTINSETGYRNMNVLDDILTPLRIYEGGALE
                     CILNRYKPAWQTFIYVYANSHEEETI"
     gene            5601..6512
                     /locus_tag="ACHKBL_004096"
     CDS             5601..6512
                     /locus_tag="ACHKBL_004096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO5820891.1"
                     /translation="MFHVDNNSGVANMPALAPAQSNTTTWFTEGDGQKGISWIGQDWL
                     NILQAELLNILAEASIQPDKAQLNQLTLSIKAIIAANAFSRKNNLKEIADAGAEAQRL
                     ARGYLGLGALATKNSLGPGDVNALAKDQNLADLENAGTARNNLDVYSKSEGDNRYLRR
                     EQNGADIPDKGAFIDNVGLRETVNKAADALPSGGTAVAANRLATPRNINGVPFDGTQD
                     INITSGMTQSTADGRYVQNVQLGAQSYHSPGGNEISWNYSAPSGCMLSGINVQETGSR
                     SADNIGGVYYRPVQIYINNAWRTVSSV"
     gene            complement(6547..7068)
                     /locus_tag="ACHKBL_004097"
     CDS             complement(6547..7068)
                     /locus_tag="ACHKBL_004097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFO5820892.1"
                     /translation="MELINVKRYYPEHKPYGEDVQYFQSEDGRDFYESIPLFTKKYKL
                     CISPVTGIICSVAEDVSALYPAGFTVVEVDELPEGVNIDGNWQFSDGLISKVPVNWKT
                     VAEKRRSSLLQEANETVDDWKTELKLDMISDENKLQLTRWMAYIRQLKEMHFNDIASE
                     GHYQAIPWPEKPE"
BASE COUNT         1569 a         1800 c         2114 g         1592 t
ORIGIN      
        1 gcgcgccggt gggttacttt cgtctgtcgg tagccgcatg gggctggggc gtttctccgg
       61 ctttttccgc agtgccggtg gactggccgg ccggctgggt ggaggtgccc tgtgggctgg
      121 cgcgatggca gccccggttc tgctggatgg cagcgccagt gccacagaca agggagaagc
      181 ggtcggctct cttgcaggca gtattgccgg tggtgcgctg ggggcggcag ccggacctgt
      241 ggggattgcc attggttcta cggtgggcag ctatctcggt aactatctgg ggggatggct
      301 gactgaagcc tggcagaaat tacggggcag tgatgatgaa agcagtggac aggcggtaca
      361 aaaagcctct gcccgtgtgg agctggttgc cccggaaggg tggcaggctc gcagtattga
      421 tattgatgac acgtccggtc acggactgga tgtgaatgtc tggaacggag ggaattatgg
      481 cctctggtga cggtcgtggt gcgttccgtg gtgtgccgtt cctcgtctgg cgtgagcagc
      541 gcgaacgtgg cgggcgaaac attgtccgtc gggaatatcc gttacgggaa accggtggtg
      601 cagacgatct gggacctaaa ctggctgaat ttacgttcag tgtacttgtg atgggggatg
      661 atgttcagac acagcgaaat cgtctgcgtg atgcccttcg tgctcccggt gccggggagc
      721 tgcttcaccc ggattacggc acgttaaatg tgctgataaa cagctttgaa agtcgttata
      781 acgctgctga acagggcgtg gttgagttca ccattaatgt cacgccggta agtgatgata
      841 ccgcacctgc tgtcacacag gacacggccg ccattctgga gcagaagagt accaccgcgc
      901 tcgggaaggt ttttgaaacc ctggaagcgg gctggacggt gatttctgac ggaatgcacg
      961 atgtccaggc aatgaccgaa accatcagcg ataaggtgtc cgccctggaa aatgccgttt
     1021 ccggcatggg gattgtacag gatatcagtg cctttactgc cacgttcacg gcgctgaaag
     1081 ggaatgccac ggcattactg acagcacctt cccgcatggc ctcatctttt gcggggcttt
     1141 tcagtgccct gatcaccctg ccatcgctgc cgtcactgtc ttcaggaggc ctgaataccc
     1201 ggcccggtgg cagtatcggc cggacgacat ccgccgtctc tcagggaatg ccgcaactgt
     1261 acaggacatt atcttccctg cgttacgtgc tggatgagca ggataatccg cagactctca
     1321 tcggtctgac accggcagca caaaagaata ttcgtctgat acgggcggta atgcagagtg
     1381 ctgccgtggt ggcccaggca cagaccgtgg gaaaactgct ggatcaggtt ctcagtcagg
     1441 aaaccctgcc ggacagtgac gcagcccacc gtacctggcc tgtctggctg gaaagttcag
     1501 ttgatcttca gcgcattaac cgtgacttaa gcgaagcgct cgaacggcag gtgatgacac
     1561 tgtccgggca ggggtatacc gccacggcgc tgacgcttcg tgatgccaga ctggcactga
     1621 cagaggatct gaatacacgg ggagttcagc tacccggtgc aacagtagtg actgtacgta
     1681 ccaccgagcc tgcactggtg accctgtacc gtgccaccgg gaacagtacc ggctggcaac
     1741 gttttgtgcg ccgtaacggt attgttgatc cgctgttcat tcccggaggc cattcagtgg
     1801 aggtgattag tgagcagcag ggttgaactg tatctgggcg gtgagatttt ttccggctgg
     1861 ctgacggtga gtgttcgtcg ctctcttgaa catctggcgg gctcctttga actgggggta
     1921 atgatgcccg gtgtacgcct tccgtcatcc gtccgtgccg gtcagtctct ggaattgcgc
     1981 attgacggtc agcctgtgat cactggctgg ctggatcagg tccggcagcg catcagcgcc
     2041 acgcgttttc agatcacgct cagcggacgg gataaaaccg gtgacctggt ggactgttca
     2101 gccattcatc cgggcagcca gtggaggaac cgcacgctgg agcacattgc ttcagatttg
     2161 tgtgctccgt tcggggtcac ggtgcgctgg caggtaaatg atgcaacggc agcccggccc
     2221 ttttccacct tcacactgga aaactcagaa accgtggcag atgcgctgac acgggccgcg
     2281 agacacagag gcgtcctggt aaccagtaat gccgccggtg aactggtttt cactcaggcc
     2341 ggcagtcagc gtggcgacac gctgacgctg ggcgaaaatc tgctggattt ggatcacaac
     2401 gtggatcacc gtctgcgcca cagtgaatac cgtgtacggg ggcacgggcg tggtggtggt
     2461 catgccgggg atgcactgac agccggaacg ctggccgcac ccgttggtac ggtgacagac
     2521 agtgccatcc accgttacag accgaaaatt gtgctggcgg atcatgctgt tgatgcagac
     2581 ggtgcacgcc agagggctgt ccgggaaatg cgccgggcgg ttgcccgctc tgtgcgcctg
     2641 acagccaccg tgcggcactg gtttcgggag aacggccagt tgtgggatat caacctgctg
     2701 acggctgtca cggctccccg caccggagtg gaagagcgtg atcttcttgt ctgtcaagtg
     2761 gagttttcgc tggatgcaaa tcacggcgaa accacccgtc tgattctggc accccgtgac
     2821 ggctttattg ttccggcaga gccgggaaac agcggaagcg gaaatgcggg tgacgtggac
     2881 gccttcgtgc gggcacagat gaaaaaacag gggattaaat tcaatgatga atgacgaagt
     2941 catcagccgc cttctggccc ccgtaatgcg gggtgttcgt ctgctgttcg ggcgtggtgt
     3001 actgaccggc acaacggaca cgctgaaaat ccagaatgtg caaatcaccg gtatggatgg
     3061 tgaaaccttt gatgacgttg aacgccccca gcagtacggg caaatcagcg tccccctgcc
     3121 tggtgcggaa gtttttctgg cctgtgctgg cggacaacgg gatcaggccg tggtgcttgt
     3181 ggtggaagac cgccgcagtc gcccgaccgg acttaccgcc ggagataccg gcgtgtatca
     3241 ccatgagggg caccgtatac gtctgacaaa gaacggccgg atcattgtga cctgtaagac
     3301 gctggagatt tacgccgatg aaggtgttca ggtggataca ccggaggctc actttaccgg
     3361 taatgtcaca gtggataaga acctgcatgt caaaggcaat gtgtccattg acggcaccgg
     3421 aagatcacag gggacgttca cgatgtccga agcggttatt gccgggatca cctattcagg
     3481 tcatgtgcat cacgataacg gtgaaggcag caaaacggga ggaccagaga atggctgata
     3541 ttgctgtcgt ctgggatcag ggttgcggtt ccctgcaact gaacggcgca gatcttctga
     3601 cggataacag cctgctgact gcggtcatta tttcactgtt tacggacagg cgggcgctgg
     3661 attccgatga aatccctgac ggcacccgtg accgtcgggg atggtgggga gacagtttcc
     3721 gggagcgccc cgttggctcc cgtctctggc tgttaagccg tgaaaagacg ctgtcctccg
     3781 tggtcagccg tgcacaggcc tatgctgatg aagcgctggc gtggctgcat aaaagcggtg
     3841 ctgccacatc cgtggtatgt catgccatgc gtgtggggca tgctcgcctt tcgctttccg
     3901 tgaaaatcac cctgccggac ggaagcagac atccgatgat tttttatgct gatatgaagg
     3961 gggaatgatg ccttatcagc ccttaccact ggcgcaactg atcacacaga cacaacagga
     4021 tatcagccag cgcctgcccg gttcgcagcc gggcgtgaat gaaaccaccc tgaatgccat
     4081 tgcgtatgca ctggcgggtc tgtcagcaca ggagcatgaa catctggcct ggatctcccg
     4141 gcagataatt ccgacagaag ctgatgaagc cgaactcctg aaacactgcg cattctgggg
     4201 tgtcatccgt aaaccggctt cccgcgctga cggaccggta caactgatgc tgaccacgga
     4261 tgcagggatc acggaaggcg tactccttca gcgaagcgat ggtgttgtgt accgcatcac
     4321 cggctctgcg accggaaaag ccggaacact gaatgttaat gtggaggcgg aaagtgcggg
     4381 gcgcgctgga aataccccga ccggaacccg cctgtccttt atcacgccac aggcgggcat
     4441 caaccagaca gccacggtca ccggcacggg actcaccggt ggtgcggatg tggaaacggt
     4501 gccggagttg ctgtccaggc tggtattccg ggtacagaac ccgccatcag ggggaacaca
     4561 gtatgatttt gaacgctggg cacgggaagt accgggcgtg acgcgggcat ggtgtaagcc
     4621 tgaatggcct gaggcgggta gtgttggtgt gacttttgtt caggataata accctgacat
     4681 tttccccgga gaaggtgatg tgaagcgggt ggcggattat atccgcagtc atgatgatcc
     4741 ggcgacgggc cagcccgttg gtcagccact tgggccgaca atcagcgtgt ttaagctgac
     4801 caataagccg gtggcgtttg agatcaggat tgtacccaaa acgccggaaa atcaggctgc
     4861 cgtaaaacag gcattaacgg acctgcttta taacgaatcg cggccgggtg gacttgtatt
     4921 gccttcatca ttctggcggg ctgttgcagg ggtgaaagga ctggaggatt ttgaagttcg
     4981 cagcccgctg aagtccgtga tggccggaga tacagagttg ctgaccgtgg gggaaatcac
     5041 atggctgtaa ccctgacccc gcatcagcgt gccctgttgc agttgctgcc tgacgggctg
     5101 gcatgggata agcggccgtc atccgttctt gcggctttgt gcctgggcct cagtcattcc
     5161 acggagcgtg tttcctggac cggtaaccag atgctggcag aacgttttcc tgattcatcc
     5221 cgtctgctgc tggaagactg ggagcgttat ctggggttac cggaatgtga tatgaccggc
     5281 gcaaccattc aggagcgtca gcgttatgcc gggaataaat accggatgaa accctctctt
     5341 aaccgtgaat tttatatccg gtttgcggca aagtttggtt atgaaataga tattcagcca
     5401 tcaccggatt cacagtgggt cagtattgtc acgattaaca gtgaaaccgg ctaccggaat
     5461 atgaatgtgc tggatgatat tctcacgccg ctgcgtattt atgaaggcgg tgcgctggaa
     5521 tgtattctga accgttataa gcctgcatgg cagacgttta tttacgtgta tgcaaacagc
     5581 catgaagagg agactattta atgtttcatg ttgataataa ttccggcgtg gcgaatatgc
     5641 ctgcgctggc accggcgcag agtaatacca ccacctggtt taccgaaggt gacggacaaa
     5701 aaggtatcag ctggattggt caggactggc tgaatattct ccaggccgaa ctgctgaata
     5761 ttctggctga agccagtatt cagccggata aggcgcagtt aaaccagctt acgctgtcca
     5821 ttaaagccat tatcgctgcg aatgcctttt cccggaaaaa caacctgaaa gaaattgctg
     5881 atgccggtgc ggaagcccag cgtcttgccc gtggttatct tggtcttggg gcgcttgcca
     5941 caaaaaacag tcttggtccc ggtgacgtta atgctctggc gaaggatcag aaccttgccg
     6001 acctggagaa tgcgggaacc gcccgtaata atctggatgt ttacagcaaa agcgaaggtg
     6061 ataaccgtta cctgcgcagg gagcagaacg gcgcagacat tccggataaa ggcgctttta
     6121 tcgataacgt cggtttacgg gaaacggtaa ataaggctgc tgatgccctg ccatcgggtg
     6181 gaaccgccgt ggcagcaaac agactcgcca ccccaagaaa tattaatggc gttccttttg
     6241 acggaacgca ggatatcaac atcacgtcag gaatgacgca gtcaaccgca gatggccggt
     6301 atgtacagaa tgttcagctt ggagcacaga gctatcattc acccggaggc aatgaaatat
     6361 catggaatta cagcgcacct tccggttgta tgctttccgg tattaacgtg caggaaaccg
     6421 gctcccggtc tgcggacaat atcggtgggg tctattatcg cccggttcag atttatatta
     6481 ataatgcctg gagaacggta agttcagtgt aaaccacaga aaagggtgct gaatgcaccc
     6541 tctgaattat tccggttttt ccggccaggg aatcgcctgg taatgcccct cactggcaat
     6601 atcattaaag tgcatttctt taagctgtcg gatatacgcc atccagcgcg tcagctgtaa
     6661 tttattttca tcgctaatca tgtccagttt taattctgtt ttccagtcat ccacggtttc
     6721 gttggcttcc tggagcagcg atgagcgacg cttttcagca accgtcttcc agtttaccgg
     6781 cacctttgat atcaggccat cagaaaactg ccagttaccg tcaatattca caccttccgg
     6841 cagttcgtcc acttcaacaa cagtaaaacc tgccggatat aaagcagaga catcttcagc
     6901 aacagagcag ataatacccg ttacaggcga aatacagagt ttgtatttct ttgtgaaaag
     6961 cgggatagat tcataaaaat cccttccgtc ctcactctgg aaatactgaa catcctcacc
     7021 atagggtttg tgttccgggt aatatcgttt tacgttaata agctccatta tttca
//
LOCUS       DAZFLD010000213         7003 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.214, whole genome shotgun sequence.
ACCESSION   DAZFLD010000213 DAZFLD010000000
VERSION     DAZFLD010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7003
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.214"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            5..223
                     /gene="yiiE"
                     /locus_tag="ACHKBL_004098"
     CDS             5..223
                     /gene="yiiE"
                     /locus_tag="ACHKBL_004098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312842.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032993 - protein-DNA complex [Evidence
                     IEA];
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HFO5820893.1"
                     /translation="MAMNTVFLHLSEEAIKRLNKLRGWRKVSRSAILREAVEQYLERQ
                     QFPVRKAKGGRQRGETVVVDDQCKEHKE"
     gene            complement(438..1367)
                     /gene="fdhE"
                     /locus_tag="ACHKBL_004099"
     CDS             complement(438..1367)
                     /gene="fdhE"
                     /locus_tag="ACHKBL_004099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HFO5820894.1"
                     /translation="MSIRIIPQDELGSSEKRTADMIPPLLFPRLKNLYNRRAERLREL
                     AENNPLGDYLRFAALIAHAQEVVLYDHPLEMDLTTRIKEASAQGKPPLDIHVLPRDKH
                     WQKLLMALIAELKPEMSGPALAVIENLEKASTQELEDMASALFASDFSSVSSDKAPFI
                     WAALSLYWAQMANLIPGKARAEYGEQRQYCPVCGSMPVSSMVQIGTTQGLRYLHCNLC
                     ETEWHVVRVKCSNCEQSGKLHYWSLDDEQAAIKAESCDDCGTYLKILYQEKEPKVEAV
                     ADDLASLVLDARMEQEGYARSSINPFLFPGEGE"
     gene            complement(1364..1999)
                     /gene="fdoI"
                     /locus_tag="ACHKBL_004100"
     CDS             complement(1364..1999)
                     /gene="fdoI"
                     /locus_tag="ACHKBL_004100"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HFO5820895.1"
                     /translation="MKRRDTIVRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLM
                     QIMGTPQLARILHPFVGVVMFASFIIMFFRYWHHNLINRDDIFWAKNIRKIVVNEEVG
                     DTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
                     IVVIMVHIYAALWVKGTITAMVEGWVTSAWAKKHHPRWYREVRKTTEKKAE"
     gene            complement(1996..2898)
                     /gene="fdoH"
                     /locus_tag="ACHKBL_004101"
     CDS             complement(1996..2898)
                     /gene="fdoH"
                     /locus_tag="ACHKBL_004101"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase O subunit beta"
                     /protein_id="HFO5820896.1"
                     /translation="MAYQSQDIIRRSATNGLTPAPQARDFQEEVAKLIDVTTCIGCKA
                     CQVACSEWNDIRDTVGNNIGVYDNPNDLSAKSWTVMRFSEVEQNDKLEWLIRKDGCMH
                     CSDPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFDIPRLNPEDNRVYKC
                     TLCVDRVVVGQEPACVKTCPTGAIHFGTKESMKTLASERVAELKTRGYDNAGLYDPAG
                     VGGTHVMYVLHHADKPNLYHGLPENPEISETVKFWKGIWKPLAAVGFAATFAASIFHY
                     VGVGPNRADEEENNLHEEKDEERK"
     gene            complement(2911..5961)
                     /gene="fdnG"
                     /locus_tag="ACHKBL_004102"
     CDS             complement(2911..5961)
                     /gene="fdnG"
                     /locus_tag="ACHKBL_004102"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0047111 - formate dehydrogenase
                     (cytochrome-c-553) activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(5374..5376),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HFO5820897.1"
                     /translation="MQVSRRQFFKICAGGMAGTTAAALGFAPSVALAETRQYKLLRTR
                     ETRNTCTYCSVGCGLLMYSLGDGAKNAKASIFHIEGDPDHPVNRGALCPKGAGLVDFI
                     HSESRLKFPEYRAPGSDKWQQISWEEAFDRIAKLMKEDRDANYIAQNAEGVTVNRWLS
                     TGMLCASASSNETGYLTQKFSRALGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANLVVVMGGNAAEAHPVGFRWAMEAKIHNGAKLIVIDPRFTRTAAVADYYAPIR
                     SGTDIAFLSGVLLYLLNNEKFNREYTEAYTNASLIVREDYGFEDGLFTGYDAEKRKYD
                     KSSWTYELDENGFAKRDTTLQHPRCVWNLLKQHVSRYTPDVVENICGTPKDAFLKVCE
                     YIAETSAHDKTASFLYALGWTQHSIGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSQSLPGYMTLPSEKQTDLQTYLTANTPKPLLEGQVNYWGNYPKFFVSM
                     MKAFFGDKATAENSWGFDWLPKWDKVYDVLQYFEMMKEGKVNGYICQGFNPVASFPNK
                     NKVIGCLSKLKFLVTIDPLNTETSNFWQNHGELNEVDSSKIQTEVFRLPSTCFAEENG
                     SIVNSGRWLQWHWKGADAPGIALTDGEILSGIFLRLRKMYAEQGGANPDQVLNMTWNY
                     AIPHEPSSEEVAMESNGKALADITDPATGAVIVKKGQQLSSFAQLRDDGTTSCGCWIF
                     AGSWTPEGNQMARRDNADPSGLGNTLGWAWAWPLNRRILYNRASADPQGNPWDPKRQL
                     LKWDGTKWTGWDIPDYSAAPPGSGVGPFIMQQEGMGRLFALDKMAEGPFPEHYEPFET
                     PLGTNPLHPNVISNPAARIFKDDAEALGKADKFPYVGTTYRLTEHFHYWTKHALLNAI
                     LQPEQFVEIGESLANKLGIAQGDTVKVSSNRGYIKAKAVVTKRIRTLKANGKDIDTIG
                     IPIHWGYEGVAKKGFIANTLTPFVGDANTQTPEFKSFLVNVEKV"
     gene            6155..6988
                     /gene="fdhD"
                     /locus_tag="ACHKBL_004103"
     CDS             6155..6988
                     /gene="fdhD"
                     /locus_tag="ACHKBL_004103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HFO5820898.1"
                     /translation="MKKTQQKEIENVTNITGVRQIELWRHDDLQHPRLDEVAEEVPVA
                     LVYNGISHVVMMASPKDLEYFALGFSLSEGIIESPRDIFGMDVVPSCNGLEVQIELSS
                     RRFMGLKERRRALAGRTGCGVCGVEQLNDIGKPVQPLPFTQTFDLNKLDDALRHLNDF
                     QPVGQLTGCTHAAAWMLPSGELVGGHEDVGRHVALDKLLGRRSQEGESWQQGAVLVSS
                     RASYEMVQKSAMCGVEILFAVSAATTLAVEVAERCNLTLVGFCKPGRATVYTHPQRLS
                     N"
BASE COUNT         1565 a         1866 c         1882 g         1690 t
ORIGIN      
        1 ggtaatggcg atgaatacgg tttttcttca tttatcagaa gaggcaatta aacggctgaa
       61 caagcttagg ggctggcgta aggtttcacg atctgcaatt ttacgcgaag cggttgagca
      121 atatctggag cggcagcaat ttccggtgcg taaagcaaag ggtggcaggc aaaggggtga
      181 gacagtagtt gttgatgatc agtgtaagga gcataaggaa tgacaaaaaa tttattattc
      241 ttgaatataa aaaccagatg cccttattct ggtattaata caagactgtt ttacttgaac
      301 ttataataac tgtaactgtt acatcatatc tggaaaacgc ctcgcaaaat acgagagatt
      361 ttgcgggaat agcaggtgtc gtcacaccct atgagctgta atccgggcga ccagcgccac
      421 ccggaaaacg tcagagatta ctccccttca cccgggaaca ggaacgggtt gatggaactg
      481 cgggcgtagc cttcttgctc cattcgcgcg tccagtacca gagaggcgag gtcatctgcc
      541 acggcttcaa ctttcggttc tttttcctga taaagaatct tcaggtaagt gccacagtca
      601 tcgcagcttt cggctttaat cgcggcctgt tcgtcatcca gcgaccagta atgcagtttg
      661 ccgctctgct cgcagttgct gcattttacg cgcaccacgt gccattcggt ttcacacagg
      721 ttgcaatgca ggtaacgcag accctgagtg gtgccaattt gcaccatgct ggacaccggc
      781 atagagccac ataccgggca atattgacgt tgttcgccgt attcagcgcg ggctttgccg
      841 gggatcagat tagccatctg cgcccagtag agcgacagcg cagcccagat aaacggcgct
      901 ttatcgctgc tgacggacga gaaatcagag gcaaacagtg cgctggccat atcttccagc
      961 tcctgagtcg atgccttctc cagattctca atcactgcca gcgccgggcc gctcatttca
     1021 ggtttcagct cagcaatcag cgccatcagc agcttttgcc agtgcttatc acgcggcaga
     1081 acgtgaatat ccagcggggg cttgccttgt gcgctggctt ctttaatgcg cgtggtcaga
     1141 tccatctcca gcggatggtc gtacagcacc acttcctggg cgtgggcgat aagcgcagca
     1201 aagcgcaggt aatcacccag cggattattt tctgccagct cgcgcagacg ctcggcgcgg
     1261 cggttgtata aattcttgag ccgagggaac aataacggcg gaatcatatc cgccgtacgt
     1321 ttctcgctcg aacccagctc atcttgcggg attatgcgaa tactcattca gctttctttt
     1381 ccgttgtctt gcggacctca cggtaccagc gcgggtgatg tttcttcgcc caggcgctgg
     1441 taacccatcc ttccaccatg gcggtaatcg tgcctttcac ccaaagggcg gcgtagatat
     1501 gcaccatgat aaccacaatt aacgccactg cggcaaatga atgcagcatt aacgcgaatc
     1561 ggatcaccgg gattgagaaa gcaggtgcaa aataaggacg ccagataatc acgccgctca
     1621 ccagcagcag aaccaggaaa ataatcgccg cccagaaaac gcatttctga ccgaagttat
     1681 aacgcccggt gtcacctact tcctcgttga cgacgatctt acgaatattc ttcgcccaaa
     1741 agatatcatc ccgattgatt aggttgtgat gccagtaacg gaaaaacatg atgatgaacg
     1801 aggcaaacat aaccacgccg acaaacgggt gcagaatgcg cgccagctgc ggtgtgccca
     1861 tgatttgcat caaccagttg aaggacggga acaaaaagcc cagcccgctc accgccgcca
     1921 ggatgaagca gaaggcggtg atccagtggt tgatacgttc cggcgcggtg tagcgcacga
     1981 tggtgtcacg tcgtttcatt tgcgctcctc gtctttctct tcgtgcagat tattctcttc
     2041 ctcatccgca cggttcggac cgacacccac gtagtggaag atactggccg caaaggtagc
     2101 cgcaaagcca acagccgcga gcggtttcca gatgcctttc cagaatttca cggtttcgct
     2161 gatttccggg ttctccggca agccatgata cagatttggc ttgtcagcat ggtgcagcac
     2221 gtacataaca tgtgtaccac cgacgcctgc cgggtcgtac aaacccgcat tgtcgtaacc
     2281 acgggttttc agctccgcca cgcgctcgct cgccagcgtt ttcatcgact ctttcgtacc
     2341 gaagtgaatc gcgcccgttg ggcaggtctt cacgcaggcc ggttcttgcc caaccaccac
     2401 gcggtcaacg cacaacgtac atttgtagac gcggttgtct tccgggttga ggcgcggaat
     2461 gtcgaacgga cagcccgcaa tgcaataacc gcagccgatg cactgctcgg actggaagtc
     2521 gacgataccg ttggcatact gaataattgc cccttccgcc gggcacgctt tcaggcagcc
     2581 tggatcggaa cagtgcatac agccgtcttt gcggatcagc cattccagtt tgtcgttctg
     2641 ctccacttcc gagaatcgca tcaccgtcca cgatttggca cttaaatcat tggggttgtc
     2701 gtacacccca atgttattgc cgacggtatc gcgaatgtcg ttccactctg aacacgccac
     2761 ctgacaggct ttacagccga tacaggtggt aacgtcgatg agtttcgcca cttcttcctg
     2821 gaagtcccgc gcctgaggcg cgggggtcag accgttagtc gcggaacgac gaatgatatc
     2881 ttgcgattga taagccatat gtcgtctccg ttacaccttt tccacattca caaggaagga
     2941 cttaaactcc ggcgtctgcg tgttcgcatc accgacgaat ggcgtcaacg tattggcaat
     3001 aaagcctttt ttcgcaacac cttcatagcc ccagtgaatc ggaataccga tggtatcgat
     3061 atctttgccg tttgctttca gcgtgcgaat acgtttggtc actaccgctt tggctttgat
     3121 atagccacgg ttggaggaga ctttcacagt atcgccctgg gcaatgccaa gtttattcgc
     3181 cagcgactcc ccgatttcca caaactgctc tggttgcaaa atcgcattca acagcgcgtg
     3241 tttggtccag tagtggaagt gctcggtcag acgataggtg gttccgacat acgggaactt
     3301 atcggcttta cccaatgctt cggcgtcgtc tttaaagata cgcgcagccg ggttcgagat
     3361 aacgtttgga tgcagcgggt tagttcccag cggcgtttca aacggctcgt agtgttccgg
     3421 gaacggacct tccgccatct tatcgagggc aaacagacgt cccatgcctt cctgctgcat
     3481 gataaacggc cctacgccgc tgcccggagg cgctgcgctg tagtccggaa tatcccagcc
     3541 ggtccactta gtgccgtccc atttcagcaa ctgacgcttc ggatcccatg ggttaccctg
     3601 cggatctgcg gaggcgcggt tatacagaat gcggcggtta agcggccatg cccatgccca
     3661 gcccagcgtg ttaccgaggc cagacggatc ggcgttatca cgacgcgcca tctggttgcc
     3721 ttccggcgtc cagctaccgg cgaaaatcca gcagccacag gaggttgtac cgtcatcgcg
     3781 cagttgagcg aacgagctaa gttgttggcc tttcttgacg ataaccgcac cggttgccgg
     3841 atcggtaata tcggccagcg ccttaccgtt gctctccatc gccacttctt ctgaagatgg
     3901 ctcatgcgga atggcgtagt tccaggtcat gttcagcacc tggtccgggt ttgcgccacc
     3961 ctgttctgca tacatcttgc gcaagcgcag gaagataccg gagaggatct cgccatcagt
     4021 cagcgcaatc cctggggcgt ccgcaccttt ccagtgccac tgtaaccagc gaccggagtt
     4081 aacgattgaa ccgttctctt ctgcgaagca agtcgatggc agacggaaca cttcggtctg
     4141 gatcttcgac gagtcaactt cgttcagctc accgtggttc tgccagaagt tagaggtttc
     4201 agtgttcagc gggtcgatgg tgacgaggaa cttcagtttc gacagacaac cgatcacttt
     4261 gtttttgttc gggaatgagg caacagggtt aaagccctgg cagatatagc cattgacctt
     4321 gccttctttc atcatctcga agtattgcag gacgtcgtag actttatccc acttcggcaa
     4381 ccaatcaaag ccccagctat tttccgccgt cgctttatca ccaaagaagg ccttcatcat
     4441 agagacgaag aatttcgggt agttgcccca gtagtttacc tggccttcca gcagcggttt
     4501 tggcgtgttg gcggtaaggt aggtttgcag atcggtctgc ttctcgctcg gcagcgtcat
     4561 gtaacccggc aggctctgcg acagcagccc caggtccgtc agcccctgaa tattggagtg
     4621 accgcgcagg gcgttaacgc cgccgcctgc catccccata ttgccgagca gcaactggat
     4681 catcgccatc gtacgaatgt tttgcgcacc aatggagtgt tgcgtccagc cgagcgcgta
     4741 aaggaacgag gcagttttat cgtgagcact ggtctcagcg atgtattcgc agactttcag
     4801 gaatgcgtct tttggcgtac cacagatgtt ttcaaccaca tctggcgtgt agcgggaaac
     4861 gtgctgtttc agcaagttcc acacgcaacg tggatgttgc agggtagtat cgcgtttggc
     4921 gaagccgttt tcgtccagtt cgtaagtcca ggaggattta tcgtacttac gtttttccgc
     4981 gtcatagccg gtgaacaggc catcttcaaa gccgtaatcc tcacgcacga tcaggctggc
     5041 gttggtatag gcttcggtgt attcgcggtt gaatttttca ttgttcagca ggtacagcaa
     5101 tacgcctgac aggaaagcaa tgtcagtacc ggaacgaata ggggcatagt agtcagctac
     5161 cgccgccgta cgcgtaaagc gaggatcgat cacaatcagc ttcgcgccat tgtgaatttt
     5221 ggcttccatc gcccagcgga acccgaccgg gtgagcttca gcggcgttac cgcccatcac
     5281 cacgacgagg ttggcgttct tgatgtcgac ccagtggttg gtcatcgcac cgcgaccaaa
     5341 tgttggagca agacttgcta ccgttggtcc gtgtcagaca cgcgcctggt tgtcgaccgc
     5401 gagcataccc agcgcgcggg agaatttttg cgttaaatag ccggtttcgt tactcgacgc
     5461 ggaagcacac agcatcccgg tggagagcca gcggttaacg gtcacgcctt cggcgttttg
     5521 cgcaatgtag ttagcatcgc ggtcttcttt catcagtttg gcgatgcgat caaacgcctc
     5581 ttcccaactg atttgctgcc atttatcaga acctggcgca cggtattccg gaaacttcag
     5641 acggctttcg gagtggatga aatccaccag gccagcgcct ttcggacaaa gtgcaccgcg
     5701 gttgaccggg tgatccgggt caccttcgat atggaagata gatgctttgg cgttttttgc
     5761 tccgtcaccg aggctgtaca tcaacagccc acagcctacg gaacaatagg tgcaggtatt
     5821 acgggtttcg cgggtgcgca gcagtttata ctgccgggtt tccgcgagtg ctacgctggg
     5881 tgcaaaaccc agtgccgctg ccgtggtgcc tgccataccg ccagcgcaga tcttaaagaa
     5941 ctgccttctg ctgacctgca tggattgctc cttgtttcga cattgtcacg tcccatttac
     6001 attcgcttgc tgcgtgtgca gggagtggga gttatttttc tttgcggaag gggccgcaaa
     6061 ggtccagaat tggctcaatt tccctccatc caggaagggt ttgtaacaga ataccataat
     6121 gttggtgtgt gtgttcttat ctggttaaga gaaagtgaaa aaaacacagc aaaaagaaat
     6181 cgaaaatgtg acaaatatca caggtgttcg tcaaattgag ttatggcggc atgatgattt
     6241 acaacaccca cggttagatg aggtggcgga agaagttccc gttgcgctgg tctacaacgg
     6301 tatttcgcat gtggtgatga tggcgtcgcc caaagacctt gagtactttg cgctcggttt
     6361 ttcgctttcc gaggggataa tcgaaagtcc gcgcgatatc ttcggcatgg atgtcgttcc
     6421 ttcctgtaat ggtcttgaag ttcaaattga gctttccagc cgccgcttta tggggttgaa
     6481 ggagcgtcgc cgggcactgg cgggacgtac gggatgtggc gtgtgcggcg tggagcaact
     6541 taatgacatc ggaaaaccgg tgcagccgct accgttcacc cagacgtttg atctcaacaa
     6601 actggatgat gcattacgtc atctcaatga ttttcagcca gtggggcaac tgaccggttg
     6661 tactcacgcc gccgcctgga tgttgccatc tggcgaactg gtcggcgggc atgaagacgt
     6721 gggtcgccat gtggcgctgg acaaactgtt aggccgccgg tcacaagaag gggaaagctg
     6781 gcagcaaggt gcggtactgg tttccagccg tgccagttat gaaatggtgc aaaagtcggc
     6841 gatgtgcggc gtagagattt tgtttgcggt gtctgccgcg accacacttg ctgtagaagt
     6901 ggctgaacgc tgtaatttga cgctggtggg cttttgtaaa ccgggtcggg caacggttta
     6961 tacccatccg cagcgtttga gcaattaatt aaaataagtc ctg
//
LOCUS       DAZFLD010000214         6967 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.215, whole genome shotgun sequence.
ACCESSION   DAZFLD010000214 DAZFLD010000000
VERSION     DAZFLD010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6967
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.215"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(19..2802)
                     /gene="pqqL"
                     /locus_tag="ACHKBL_004104"
     CDS             complement(19..2802)
                     /gene="pqqL"
                     /locus_tag="ACHKBL_004104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFO5820899.1"
                     /translation="MRNLCFLLMLVATLLLPGRLIAAALPQDEKLITGQLDNGLRYMI
                     YPHAQPKDQVNLWLQIHTGSLQEEDNERGVAHFVEHMMFNGTKTWPGNKVIETFESMG
                     LRFGRDVNAYTSYDETVYQVSLPTTQKQNLQQVMAIFSEWSNAATFEKLEVDAERGVI
                     TEEWRAHQDAKWRTSQARRPFLLANTRNLDREPIGLMDTVATVTPAQLRQFYQRWYQP
                     NNMTFIVVGDIDSKEALALIKDNLSKLPSNKAAENRVWPTKAENHLRFNIINDKENRV
                     NGIALYYRLPMVQVNDEQSFIEQAEWSMLVQLFNQRLQERIQSGELKTISGGTARSVK
                     IAPDYQSLFFRVNAQDDNMQDAANALMAELATIDQHGFSAEELDDVKSTRLTWLKNAV
                     DQQAERDLRMLTSRLASSSLNNTPFLSPEETYQLSKRLWQQITVQSLAEKWQQLRKNQ
                     DAFWEQMVNNEVAAKKALSPAAILALEKEYANKKLAAYIFPGRNLSLTVDADPQAEIS
                     SKETLAENLTSLTLSNGARVILAKSAGEEQKLQITAVSNKGDLSFPAQQKSLIALANK
                     AVSGSGVGELSSSSLKRWSAENSVTMSSKVSGMNTLLSVSARTNNPEPGFQLINQRIT
                     HSTINDNIWASLQNAQIQALKTLDQRPAEKFAQQMYETRYADDRTKLLQENQIAQFTA
                     ADALAADRQLFSSPADITFVIVGNVAEDKLLPLITLYLGSIKHSDSPLAAGKPLTRAT
                     DNASVTVKEQNEPVAQVSQWKRYDSRTPVNLETRMALDAFNVALAKDLRVNIREQASG
                     AYSVSSRLSVDPQAKDISHLLAFTCQPERHDELLTLANEVMVKRLAKGISEQELNEYQ
                     QNVQRSLDIQQRSVQQLANTIVNSLIQYDDPAAWTEQEQLLKQMTVENVNTAVKQYLS
                     HPVNTYTGVLLPK"
     gene            complement(2859..5231)
                     /gene="yddB"
                     /locus_tag="ACHKBL_004105"
     CDS             complement(2859..5231)
                     /gene="yddB"
                     /locus_tag="ACHKBL_004105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HFO5820900.1"
                     /translation="MKRVLIPGVILCGADVAQAVDDKNMYMHVFEEMTVYAPVPVPVN
                     GNTHYTSESIERLPTGNGNISDLLRTNPAVRMDSTQSTSLNQGDIRPEKISIHGASPY
                     QNAYLIDGISATNNLNPANESDASSATNISGMSQGYYLDVSLLDNVTLYDSFVPVEFG
                     RFNGGVIDAKIKRFNADDSSVKLGYRTTRSDWLTSHIDENNKSAFNQGSSGSTYYSPD
                     FKKNFYTLSFNQELADNFGVTAGLSRRQSDITRADYVSNDGIVAGRAQYKNVIDTALS
                     KFTWFASDRFTHDFTLKYTGSSRDYNTSTFPQSDREMGNKSYGLAWDMDTQLAWAKLR
                     TTVGWDHISDYTRHDHDIWYTELSCTYGDISGRCTRGGLGHISQAVDNYTFKTRLDWQ
                     KFAVGDVSHQPYFGAEYIYSDAWTERHNQSESYVINAAGKKTNHTIYHKGKGSLGIDN
                     YTLYMADRISWRNVSLMPGVRYDYDNYLSNHNISPRFMTEWDIFADQTSMITAGYNRY
                     YGGNILDMGLRDIRNSWTESVSGNKTLTRYQDLKTPYNDELAMGLQQKIGKNVIARAN
                     YVYREAHDQISKSSRTDSATKTTITEYNNDGKTKTHSFNLSFELAEPLHISQVDVNPQ
                     IVFSYIKSKGNLSLNNGYEESNTDDNRVVYNGNLVSYDSVPVADFNNPLKISLNMDFT
                     HQPSGLVWANTLTWQEARKARIILGKTNAQYINEYSDYKQYVDEKLDSSLTWDTRLSW
                     TPQFLKQQNLTISADILNVLDSKTAVDTTNTGVATYASGRTFWLDVSMKF"
     gene            complement(5269..6954)
                     /gene="yddA"
                     /locus_tag="ACHKBL_004106"
     CDS             complement(5269..6954)
                     /gene="yddA"
                     /locus_tag="ACHKBL_004106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HFO5820901.1"
                     /translation="MITIPITFCMLIAKYLCLLKPFWLRKNNKTSVLLIIIILAMILG
                     VVKIQVWLNDWNNDFFNALSQKETDKLWQLVLWFPALLGIFVLISVNKTWLIKLLTIR
                     WREWLTDYYLNRWFADKNYYFTQIYGEHKNTDNPDQRIAEDILLLISKTLSLSFGFIQ
                     SLSMLITFTVILWQSAGTLSFTVGGTEWNIQGYMVYTVVLIVIGGTLFTHKVGKRIRP
                     LNVEKQRSEATFRTNLVQHNKQAELIALSNAEYLQRQELSDNFHTIKENWHRLMNRQR
                     WLDYWQNIYSRSLSVLPYFLLLPQFISGQINLGGLMKSRQAFMLVSNNLSWFIYKYDE
                     LAELAAVIDRLYEFHQLTEQRPTNKPKNCQHAVQVADASIRTPDNKIILENLNFHVSP
                     GKWLLLKGYSGAGKTTLLKTLSHCWPWFKGDISSPADSWYVSQTPLIKTGLLKEIICK
                     ALPLSVDDKSLSEVLHQVGLGKLAARIHDHDRWGDILSSGEKQRIALARLILRRPKWI
                     FLDETTSHLEEQEAIRLLRLVREKLPTSGVIMVTHQPGVWNLADDICDISAVI"
BASE COUNT         1800 a         1597 c         1572 g         1998 t
ORIGIN      
        1 gtgttgacgg ggctgttatt attttggcaa taatactccg gtataggtat tcaccggatg
       61 agaaagatat tgtttaacgg cagtgttaac attctctacc gtcatttgtt tcaataattg
      121 ctcctgctca gtccatgctg caggatcgtc atattgaata agactattta caatggtgtt
      181 cgctaattgt tgaacgctac gctgttggat atcgaggctg cgctgaacgt tttgctggta
      241 ttcgttcagt tcttgctcac tgatcccttt agccagacgc ttaaccatca cttcattcgc
      301 taacgttaac agttcatcat gtcgttccgg ttgacaagta aaagccagca aatgactgat
      361 atctttggcc tgaggatcaa ccgagaggcg agaagaaacg ctgtatgccc cagaggcctg
      421 ttcacgaata ttaacgcgta gatcttttgc cagtgcgacg ttaaaagcat cgagcgccat
      481 acgcgtctcc agattaaccg gtgtccggga atcataacgc ttccactgtg aaacctgtgc
      541 cacaggttca ttttgttctt ttacagtaac cgatgcgttg tccgtcgcgc gagttaatgg
      601 tttacctgcg gctaatggcg aatcagagtg tttgattgat cctaagtaaa gcgtaattaa
      661 cggcaggagt ttgtcttctg cgacattacc gacaatgaca aacgtgatat ccgctggaga
      721 tgaaaacaac tggcgatcgg cagccagcgc atctgcggca gtaaactgtg caatctgatt
      781 ttcttgcagt aatttcgtgc ggtcatcagc atagcgcgtc tcatacatct gctgggcgaa
      841 tttctccgct ggacgctggt cgagcgtttt caacgcctga atttgagcat tttgtagcga
      901 tgcccaaata ttatcgttaa tcgtgctgtg ggtgattcgc tggttaatca actggaaacc
      961 aggttcaggg ttattagtcc gcgcgctaac agagagcaat gtattcatgc cactgacttt
     1021 actgctcatg gttaccgagt tttccgcact ccagcgtttc aggctggaag aggagagttc
     1081 accaacgccg cttccgctta ctgctttatt tgccagcgcg ataagtgatt tttgctgcgc
     1141 agggaaactt aaatcgcctt tattagatac ggcagtaatt tgtagctttt gctcttcacc
     1201 cgcggatttt gccagaataa ccctggcacc attggaaagt gttaatgatg tcagattctc
     1261 agccagcgtt tctttgctgc taatttccgc ctgtgggtca gcgtctactg ttaacgataa
     1321 atttctgcct gggaagatgt aagccgccag ctttttgttg gcgtactctt tttccagcgc
     1381 caggatagcc gcaggagaca atgctttttt ggcggcaact tcattgttta ccatttgctc
     1441 ccaaaatgcg tcctggttct ttcttaactg ctgccatttt tccgccagac tttgcacggt
     1501 aatttgctgc cacagacgtt tcgaaagttg atatgtctct tccggcgaca agaacggcgt
     1561 attatttaat gagctggatg ccaggcgact ggtcagcata cgtaaatcac gctcagcttg
     1621 ctgatcaacc gcatttttca gccaggtaag gcgggtagat ttaacatcat cgagttcttc
     1681 agcagaaaag ccatgctgat caatggttgc caactctgcc attaatgcat tcgcagcatc
     1741 ctgcatatta tcgtcttgtg catttacacg gaaaaacaaa gactgataat cgggtgcaat
     1801 tttgacgctg cgcgcagtgc cgccagaaat agtcttcaac tcgcccgact gtatgcgttc
     1861 ttgcagacgt tggttgaaca gctgaaccaa catgctccat tcagcttgtt cgataaagct
     1921 ttgctcatcg ttcacttgta ccattggcag gcgataatag agtgcgatgc cgttcacccg
     1981 gttttcttta tcattgatga tattaaagcg caggtggttt tcggcttttg tcggccagac
     2041 gcgattttca gctgctttgt tagacggaag cttacttaaa ttatccttaa tcagcgccag
     2101 cgcttcttta ctgtcaatat cgccgaccac gataaaggtc atattatttg gttgatacca
     2161 gcgttgataa aattggcgca attgtgccgg tgtgaccgta gcgacagtat ccatcaggcc
     2221 gataggttca cggtctaaat tacgggtatt tgccagcagg aaagggcggc gcgcctgaga
     2281 ggtgcgccat ttcgcatcct gatgggcacg ccattcctca gtaattacgc cgcgttcagc
     2341 gtctacttcg agtttttcaa aggttgcggc attactccat tcactgaaga ttgccatcac
     2401 ttgttgcaga ttttgtttct gcgtagtcgg caaactcacc tgatacactg tttcgtcata
     2461 gctggtatag gcattaacat cgcgaccaaa acgcaggccc attgactcaa atgtttcgat
     2521 gactttatta cccggccatg tttttgtgcc gttaaacatc atatgttcta caaaatgagc
     2581 cacgccgcgc tcattgtcct cttcctgcaa tgaaccggta tgaatttgca gccataaatt
     2641 tacctgatcc tttggttgag catgcggata aatcatatat cgcaagccat tgtccagttg
     2701 cccggtaatt aacttttcat cctgcggtaa ggcggcggca atcagtcgcc cggggagcaa
     2761 cagagttgcc actaacatca gtaagaaaca gaggtttctc ataattatct ccatgcgaaa
     2821 accgggcaaa tttacccggt aaagtaaaat ccgaactatt aaaatttcat gctgacatca
     2881 agccagaaag tacggccact ggcgtaggtc gccacgccgg tattcgttgt atcaaccgcg
     2941 gttttgctat ccagtacatt gagaatatcg gcactgatcg tcaggttttg ttgtttcaga
     3001 aattgtggcg tccaggacaa gcgggtgtcc caggtcaggc tgctatccag tttctcgtca
     3061 acatactgct tgtaatctga atattcgttg atgtattgcg cattcgtctt acccaggata
     3121 atgcgagctt tacgcgcttc ttgccaggtc agcgtattcg cccacaccaa cccgctcggt
     3181 tgatgcgtga aatccatgtt taaggagatc tttaatggat tattaaaatc cgccactgga
     3241 acgctatcgt aagagaccag attaccgtta taaaccaccc ggttatcatc ggtattgctt
     3301 tcctcataac cattgtttaa cgacaagttg cccttgctct tgatatagct aaagacaatt
     3361 tgcgggttaa catctacctg gctgatatgc aggggttcgg ccagttcaaa actgaggttg
     3421 aacgaatgcg ttttggtttt gccgtcgttg ttatattcag taatggtggt tttagtcgcg
     3481 ctgtcggtac gactgctttt gctgatttga tcatgcgctt cacggtaaac atagtttgcg
     3541 cgcgcaataa cgttcttacc gattttctgc tgcaacccca ttgccagttc atcgttataa
     3601 ggcgttttca aatcctgata acgcgtcagg gttttattgc ctgataccga ttccgtccag
     3661 ctattgcgga tatcacgtaa tcccatatca agaatattcc cgccatagta acggttataa
     3721 cctgcggtaa tcattgaggt ttgatcagca aaaatatccc attccgtcat aaagcgcggg
     3781 gagatattgt ggtttgacag atagttgtca tagtcatacc gcacaccggg cattaacgac
     3841 acattccgcc agctaatgcg atccgccata tacagcgtgt agttgtcaat tcccagactg
     3901 cctttacctt tatggtaaat ggtatggtta gttttctttc ctgcagcatt aatcacatag
     3961 gattcagact ggttatggcg ttcagtccat gcatcggaat agatgtattc cgcgccgaag
     4021 tagggttgat gcgaaacatc acccacggcg aatttttgcc agtccaggcg tgttttgaag
     4081 gtgtaattat ctacagcctg ggaaatgtgt cctaatccac cacgggtgca acgacctgaa
     4141 atatcaccat atgtacatga aagttcggtg taccagatgt catgatcgtg acgggtataa
     4201 tcactaatat gatcccaacc aacggtagta cgtagtttgg cccatgcgag ctgcgtatcc
     4261 atatcccatg ccagaccgta ggatttatta cccatttcgc gatcagactg cgggaaggtg
     4321 ctggtattat aatcacgact ggagccggta tattttaagg taaaatcgtg ggtaaagcgg
     4381 tcgctggcaa accaagtaaa tttgctcaat gcggtatcga taacgttttt atactgtgcc
     4441 cgaccggcga caatgccgtc attcgaaaca taatccgcgc gggtgatatc agactggcgg
     4501 cgcgataaac cggcggtaac gccaaagtta tcagcgagtt cctgattaaa cgacaaggta
     4561 taaaagttct ttttaaaatc tggagagtaa taagtacttc ctgaagaacc ttgattaaat
     4621 gcgctcttgt tattctcatc gatatgcgat gttaaccagt ccgaacgcgt agtgcgataa
     4681 cccagtttca cgctgctatc atcagcgttg aagcgtttga tctttgcatc aattaccccg
     4741 ccattgaagc gaccgaattc aaccggcaca aaactgtcat aaagcgtcac attgtccagt
     4801 aagctgacat caagataata gccctgtgac atcccgctaa tattggttgc actactggca
     4861 tcggactcat tcgctgggtt caggttatta gttgcactaa taccatcaat caaataggca
     4921 ttctggtagg gcgacgcacc gtgaatagag attttctccg ggcgaatatc tccctggttc
     4981 aatgaggtac tttgcgttga atccatgcgc accgcagggt tggttctcag cagatcgctg
     5041 atattgccat tcccggtcgg taaacgctcg atgctttcac tggtgtaatg cgtgttgccg
     5101 tttacgggta cagggacagg agcatagacc gtcatctctt caaaaacatg catgtacatg
     5161 tttttgtcat cgacggcctg cgctacatca gcgccacata aaatgacgcc aggaataaga
     5221 actcgcttca tttatttata tctccgtaca gcagggcttt tattgttttt atataaccgc
     5281 gctaatgtca caaatatcat cagccaggtt ccagacaccg ggttgatgtg taaccataat
     5341 gacgccgctt gtgggtagtt tttcacgcac taaacgcagt aagcggatag cctcttgttc
     5401 ctcaagatga gaggtagttt cgtcaagaaa tatccatttc ggacgtcgta aaattaatcg
     5461 tgccagggcg atacgttgtt tttcgccgct ggaaagaata tcgccccagc gatcgtggtc
     5521 atgaatacgc gcagccaatt tccccagacc aacctgatgc agtacttcgc tcaacgattt
     5581 atcgtctacg gacaggggaa gtgctttaca aattatctct ttcagtaagc cggttttgat
     5641 taacggtgtt tgtgacacat accaactgtc agcaggagaa gaaatatcac ctttaaacca
     5701 cggccagcag tgggataatg ttttaagcag tgtggttttt cccgcaccag agtaaccttt
     5761 cagtaatagc catttgcctg gcgaaacatg aaagttcagg ttctctaata tgatcttatt
     5821 atcaggagta cgaatactcg cgtcagccac ttgtaccgca tgttggcaat ttttaggctt
     5881 attcgtaggg cgctgttcgg tgagttgatg gaactcatac aaacgatcga taaccgcagc
     5941 cagttcagca agttcgtcat atttataaat aaaccagctt aaattgttcg ataccagcat
     6001 aaatgcctgg cgcgatttca tcagtccgcc cagattgatc tgtccgctaa taaactgcgg
     6061 caataacaga aagtagggaa gaacgctaag cgagcgcgaa taaatattct gccagtaatc
     6121 aagccaccgc tgacgattca ttaaacgatg ccagttctct ttaatggtat gaaaattgtc
     6181 gcttaactcc tggcgttgta gatactcagc attgcttagc gcaatcagtt ctgcctgctt
     6241 attatgctgc acaagattag ttcgaaatgt cgcttcgcta cgttgttttt ccacattaag
     6301 cggacgaata cgtttaccaa ccttatgggt aaataaagtt ccaccgatca caatgagcac
     6361 aacggtatag accatatacc cctggatatt ccattctgtt ccaccaacag tgaatgagag
     6421 cgtacccgcg ctttgccaaa gaataacggt aaaggtgatc aacatgctaa gtgactggat
     6481 gaagccaaat gaaaggctga gcgttttact gatcagtaag agaatgtcct cagcgatacg
     6541 ttggtcaggg ttatctgtat ttttatgctc accgtagatc tgcgtgaagt agtagttttt
     6601 atctgcaaac cagcgattaa ggtagtaatc tgttaaccat tcgcgccagc gaatagtcag
     6661 tagtttaatt agccatgttt tgtttacgga tattaataca aagatcccca gcagggcagg
     6721 gaaccaaagg acaagctgcc aaagcttatc ggtttctttc tggcttagcg cattgaaaaa
     6781 atcattattc cagtcgttta accagacctg aattttaacc acgccaagga tcatggcgag
     6841 gataataatg attaacaaca ctgaagtttt attattctta cgcagccaaa atggcttcag
     6901 cagacagaga tactttgcta tcaacataca aaacgtaatg ggaatggtta tcattagcga
     6961 aaattga
//
LOCUS       DAZFLD010000215         6930 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.216, whole genome shotgun sequence.
ACCESSION   DAZFLD010000215 DAZFLD010000000
VERSION     DAZFLD010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6930
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.216"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            469..1458
                     /gene="araF"
                     /locus_tag="ACHKBL_004107"
     CDS             469..1458
                     /gene="araF"
                     /locus_tag="ACHKBL_004107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter substrate-binding
                     protein AraF"
                     /protein_id="HFO5820902.1"
                     /translation="MHKFTKALAAIGLAAVMSQSAMAENLKLGFLVKQPEEPWFQTEW
                     KFADKAGKDLGFEVIKIAVPDGEKTLNAIDSLAASGAKGFVICTPDPKLGSAIVAKAR
                     GYDMKVIAVDDQFVNAKGKPMDTVPLVMMAATKIGERQGQELYKEMQKRGWDVKESAV
                     MAITANELDTARRRTTGSMDALKAAGFPEKQIYQVPTKSNDIPGAFDAANSMLVQHPE
                     VKHWLIVGMNDSTVLGGVRATEGQGFKAADIIGIGINGVDAVSELSKAQATGFYGSLL
                     PSPDVHGYKSSEMLYNWVAKDVEPPKFTEVTDVVLITRDNFKEELEKKGLGGK"
     gene            1528..3042
                     /gene="araG"
                     /locus_tag="ACHKBL_004108"
     CDS             1528..3042
                     /gene="araG"
                     /locus_tag="ACHKBL_004108"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter ATP-binding protein
                     AraG"
                     /protein_id="HFO5820903.1"
                     /translation="MQQSTPYLSFRGIGKTFPGVKALTDISFDCYAGQVHALMGENGA
                     GKSTLLKILSGNYAPTTGSVVINGQEMSFSDTTAALNAGVAIIYQELHLVPEMTVAEN
                     IYLGQLPHKGGIVNRSLLNYEAGLQLKHLGMDIDPDTPLKYLSIGQWQMVEIAKALAR
                     NAKIIAFDEPTSSLSAREIDNLFRVIRELRKEGRVILYVSHRMEEIFALSDAITVFKD
                     GRYVKTFTDMQQVDHDALVQAMVGRDIGDIYGWQPRSYGEERLRLDAVKAPGVRTPIS
                     LAVRSGEIVGLFGLVGAGRSELMKGMFGGTQITAGQVYIDQQPIDIRKPSHAIAAGMM
                     LCPEDRKAEGIIPVHSVRDNINISARRTHVLGGCVINNGWEENNADHHIRSLNIKTPG
                     ADQLIMNLSGGNQQKAILGRWLSEEMKVILLDEPTRGIDVGAKHEIYNVIYALAAQGV
                     AVLFASSDLPEVLGVADRIVVMREGEIAGELLHEQADERQALSLAMPKVSQAVA"
     gene            3057..4043
                     /gene="araH"
                     /locus_tag="ACHKBL_004109"
     CDS             3057..4043
                     /gene="araH"
                     /locus_tag="ACHKBL_004109"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter permease AraH"
                     /protein_id="HFO5820904.1"
                     /translation="MSSVSTSGSGAPKSSFSLGRIWDQYGMLVVFAVLFIACAIFVPN
                     FATFINMKGLGLAISMSGMVACGMLFCLASGDFDLSVASVIACAGVTTAVVINLTESL
                     WIGVAAGLLLGVLCGLVNGFVIAKLKINALITTLATMQIVRGLAYIISDGKAVGIEDE
                     SFFALGYANWFGLPAPIWLTVACLIIFGLLLNKTTFGRNTLAIGGNEEAARLAGVPVV
                     RTKIIIFVLSGLVSAIAGIILASRMTSGQPMTSIGYELIVISACVLGGVSLKGGIGKI
                     SYVVAGILILGTVENAMNLLNISPFAQYVVRGLILLAAVIFDRYKQKAKRTV"
     gene            4210..5010
                     /gene="otsB"
                     /locus_tag="ACHKBL_004110"
     CDS             4210..5010
                     /gene="otsB"
                     /locus_tag="ACHKBL_004110"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HFO5820905.1"
                     /translation="MTEPLTETPELSAKYAWFFDLDGTLAEIKPHPDQVVVPDTILQG
                     LQLLATASDGALALISGRSMVELDALAKPYRFPLAGVHGAERRDINGKTHIVHLPDAI
                     ARDISVQLHTAIAQFPGAELETKGMAFALHYRQAPQHEDALMTLAQRITQIWPQMALQ
                     QGKCVVEIKPRGTSKGEAIAAFMQEAPFIGRTPVFLGDDLTDESGFAVVNRLGGMSVK
                     IGTGATQASWRLAGVPDVWSWLEMITTALQQKRENNRSDDYESFSRSI"
     gene            4985..6409
                     /gene="otsA"
                     /locus_tag="ACHKBL_004111"
     CDS             4985..6409
                     /gene="otsA"
                     /locus_tag="ACHKBL_004111"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003825 - alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming) activity [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalose-phosphate synthase"
                     /protein_id="HFO5820906.1"
                     /translation="MSRLVVVSNRIAPPDEHAASAGGLAVGILGALKAAGGLWFGWSG
                     ETGNEDQPLKKVKKGNITWASFNLSEQDLDEYYNKFSNAVLWPAFHYRLDLVQFQRPA
                     WDGYLRVNALLADKLLPLLQDDDIIWIHDYHLLPFAHELRKRGVNNRIGFFLHIPFPT
                     PEIFNALPTYDTLLEQLCDYDLLGFQTENDRLAFLDCLSNLTRVTTRSAKSHTACGKA
                     FRTEVYPIGIEPKEIAKQAAGPLPPKLAQLKAELKNVQNIFSVERLDYSKGLPERFLA
                     YEALLEKYPQHHGKIRYTQIAPTSRGDVQAYQDIRHQLENEAGRINGKYGQLGWTPLY
                     YLNQHFDRKLLMKIFRYSDVGLVTPLRDGMNLVAKEYVAAQDPANPGVLVLSQFAGAA
                     NELTSALIVNPYDRDEVAAALDRALTMSLAERISRHAEMLDVIVKNDINHWQECFISD
                     LKQIVPRSAESQQRDKVATFPKLA"
     gene            complement(6416..6844)
                     /gene="uspC"
                     /locus_tag="ACHKBL_004112"
     CDS             complement(6416..6844)
                     /gene="uspC"
                     /locus_tag="ACHKBL_004112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspC"
                     /protein_id="HFO5820907.1"
                     /translation="MSYSNILVAVAVTPESQQLLAKAVSIARPVKGHISLITLASDPE
                     MYNQLAAPMLEDLRSVMQEETQSFLDKLIQDAGYPVDKTFIAYGELSEHILEVCRKYH
                     FDLVICGNHNHSFFSRASCSAKRVIASSEVDVLLVPLTGD"
BASE COUNT         1733 a         1643 c         1811 g         1743 t
ORIGIN      
        1 atggctctca ttatacgtgt tctgttaatt aatgagcaca gtgataatta atggcgaaat
       61 gaatgacgca cattttccac atctttgagt tgtggttatt acaccattgc aaaaactcaa
      121 cgccaggtaa tgcggcctat tgactggtta aaaagaagac atcccgcatg ggtaccaaag
      181 acaacaagga cttccaggct aatcttatgg attattctgc tgtgcactcg ataatttgtc
      241 tgacaaattc gcttttcctt atgtcttttc ccgctaaatt tatgcacgtt ctcactgtaa
      301 ttctgcgatg tgatattgct ctcctatgga gaattaattt ctcgctaaaa ctatgtcaac
      361 acagtcactt atcttttagt taaaaggtaa tgctttgttt tccgattaat ttaacgaatg
      421 tcattcgttt ttgccctaca caaaacgaca ctaaagctgg agagaaccat gcacaaattt
      481 actaaagccc tggcagccat tggtctggca gccgttatgt cacaatccgc tatggcggag
      541 aacctgaagc tcggtttcct ggtgaagcaa ccggaagagc cgtggttcca gaccgaatgg
      601 aagtttgccg ataaagccgg gaaagattta gggtttgaag ttattaagat tgccgtgccg
      661 gatggcgaaa aaacattgaa cgctatcgac agcctggctg ccagtggcgc gaaaggtttc
      721 gttatttgta ctccggaccc gaaactcggc tctgccatcg tggcgaaagc gcgtggctac
      781 gatatgaaag tcattgccgt ggatgaccag tttgttaacg ccaaaggtaa gccaatggat
      841 accgttccgc tggtgatgat ggctgcgacc aaaattggcg aacgtcaggg ccaggaactg
      901 tataaagaga tgcagaaacg tggctgggat gtcaaagaaa gcgcggtgat ggcgattacc
      961 gccaacgaac tggataccgc ccgccgccgt acaacgggtt ctatggacgc actgaaagct
     1021 gcaggattcc cggaaaaaca aatttatcag gtacctacca aatctaacga catcccgggg
     1081 gcatttgacg ctgccaactc aatgctggtt caacatccgg aagttaaaca ttggctgatc
     1141 gtcggtatga acgacagcac cgtgctgggc ggcgtacgcg cgacggaagg tcagggcttt
     1201 aaagcggccg atatcatcgg tatcggtatt aacggtgtgg atgcggtgag cgaactgtct
     1261 aaggcacagg caaccggctt ctacggttcc ctgctgccaa gcccggacgt acatggttat
     1321 aagtccagcg aaatgcttta caactgggta gcaaaagacg ttgaaccgcc aaaatttacc
     1381 gaagttaccg acgtggtgct gatcacgcgt gacaacttta aagaagaact ggagaaaaaa
     1441 ggtttaggcg gtaagtaatt tgccggaaag attcccctct gcatgatgca gagggggtgt
     1501 gaacgaccag tgattcacgg agacgttatg caacagtcta ccccgtatct ctcatttcgc
     1561 ggcatcggta aaacgtttcc cggcgttaag gcgctgacgg atattagttt tgactgctat
     1621 gccggtcagg ttcatgcgtt gatgggtgaa aatggcgcag gaaaatcaac tctcttaaaa
     1681 atcctcagcg gaaactatgc gccaaccacg ggttctgtag tgattaatgg gcaggaaatg
     1741 tccttttccg acacgaccgc agcacttaac gcgggggtgg cgattattta ccaggagctg
     1801 catctcgtgc cggaaatgac cgtcgcggaa aacatctatc tcggccagct gccgcataaa
     1861 ggcggcattg tcaatcgctc attgctgaat tatgaggcgg gtttacaact taaacatctt
     1921 ggtatggata ttgacccgga cacgccgctg aaatatctct ccattggtca gtggcagatg
     1981 gttgaaattg ccaaagccct ggcgcgtaat gccaaaatta tcgcctttga tgagccaacc
     2041 agctccctct ctgcccgtga aatcgacaat cttttccgcg ttattcgtga actgcgaaaa
     2101 gaggggcggg tgatcttata cgtttctcac cgtatggaag aaatatttgc cctcagcgat
     2161 gccatcactg tctttaaaga tggacgttat gtcaaaacct ttaccgatat gcagcaggtt
     2221 gaccacgacg ccctggtgca ggcgatggtc gggcgcgaca tcggcgatat ctacggttgg
     2281 caaccgcgta gttatggcga ggagcggctg cgtcttgatg ctgtgaaagc accaggcgtg
     2341 cgtacgccaa taagtctggc ggttcgcagt ggagaaattg tcggtctgtt tggtctggtc
     2401 ggggcagggc gtagcgaatt aatgaaaggc atgtttggcg gtacgcaaat caccgccggt
     2461 caggtttata tcgaccaaca gccgatcgat attcgtaaac cgagccacgc cattgccgca
     2521 ggcatgatgc tctgcccgga agatcgcaaa gcggaaggca ttattcccgt gcactccgtt
     2581 cgcgacaata tcaacatcag tgccagacgt acacatgtgc tcggcggttg tgtaatcaac
     2641 aacggttggg aagaaaacaa tgccgatcac cacattcgtt cgctcaacat caaaacgccg
     2701 ggcgctgatc aactgatcat gaatctctca ggcggaaatc agcaaaaagc cattctgggc
     2761 cgctggttat cggaagagat gaaggtcatt ttgctggatg aacctacgcg cggcattgat
     2821 gttggtgcta agcacgaaat atataacgta atttatgcgc tggcggcgca gggtgtggcg
     2881 gtgctgtttg cctccagcga cttacctgaa gtcctcggcg ttgccgaccg gattgtggtg
     2941 atgcgggaag gcgaaatcgc cggtgaattg ttacacgagc aggcagatga gcgtcaggca
     3001 ctgagccttg cgatgcctaa agtcagccag gcagttgcct gagtaaggag agtatgatgt
     3061 cttctgtttc tacatcgggt tctggcgcac ctaagtcgtc attcagctta gggcgtatct
     3121 gggatcagta cggcatgctg gtggtgtttg cggtcctttt tatcgcctgt gccatttttg
     3181 tcccgaactt tgccaccttt attaatatga aagggttggg tctggcaatt tccatgtcgg
     3241 ggatggtggc ttgtggcatg ttgttctgcc ttgcttccgg tgactttgac ctttctgtcg
     3301 cctccgtaat tgcctgtgcg ggtgtcacca cggcggtggt tattaacctg actgaaagcc
     3361 tgtggattgg cgtggcagcg ggtttgttgc tgggcgttct ctgtggcctg gtcaatggct
     3421 ttgttattgc caaactgaaa ataaatgccc tgatcacgac actggcaacg atgcagattg
     3481 ttcgaggtct ggcgtacatc atttcagacg gtaaagcggt cggtatcgaa gatgaaagct
     3541 tctttgccct tggttacgcc aactggttcg gtctgcctgc gccaatctgg ctcaccgtcg
     3601 cgtgtctgat catctttggc ttgctgctga ataaaaccac ctttgggcgt aataccctgg
     3661 cgattggcgg gaacgaagag gccgcgcgtc tggcgggtgt accggttgtt cgcaccaaaa
     3721 ttattatctt tgttctctct ggcctggtat cagcgatagc cggaattatt ctggcttcac
     3781 gtatgaccag tgggcagcca atgacgtcga ttggctatga gctgattgtg atctccgcct
     3841 gcgttttagg tggcgtttct ctgaaaggcg gcatcggaaa aatctcatat gtggtggcgg
     3901 gtatcttaat tttaggcacc gtggaaaacg ccatgaacct gcttaatatt tctcctttcg
     3961 cacagtacgt agttcgcggc ttaatcctgc tggcagcggt gattttcgac cgttacaagc
     4021 aaaaagcgaa acgcactgtc tgatgctttt ttccacaaca atttagcgtt ttttcccacc
     4081 acagccagcc gccacaacgg ttggctgttc ttcgttgcaa atggcgaccc ccgtcacact
     4141 gtctatactt acatgtctgt aaagcgcgtt ctgcgcaaca caataagaaa agagaaggag
     4201 gagaaccggg tgacagaacc gttaaccgaa acccctgaac tatccgcgaa atatgcctgg
     4261 ttttttgatc tcgatggaac gctggcggaa atcaaaccgc atcccgatca ggtcgtcgtg
     4321 cctgacacta ttctgcaagg acttcagcta ctggcaaccg caagtgatgg tgcattggca
     4381 ttgatttcag ggcgctcaat ggtggagctt gacgcactgg caaaacctta tcgcttcccg
     4441 ttagcgggcg tgcatggggc ggagcgccgt gacatcaatg gtaaaacgca tatcgttcat
     4501 ctgccggatg cgattgcgcg tgatattagc gtgcaactgc atacggctat cgcccagttt
     4561 cccggtgcgg agctggagac gaaagggatg gcctttgcgc tgcattatcg tcaggctccg
     4621 cagcatgaag acgcattaat gacattagcg caacgtatta ctcagatctg gccacaaatg
     4681 gcgttacagc agggaaagtg tgttgtcgag atcaaaccgc gaggcaccag taaaggtgag
     4741 gcgattgctg cctttatgca ggaagctccc tttatcgggc gaacgcctgt atttctgggc
     4801 gatgatttaa ccgatgaatc tggcttcgca gtcgttaacc ggctgggcgg aatgtcagta
     4861 aaaattggca caggcgcaac tcaggcatca tggcgactgg cgggcgtgcc ggatgtctgg
     4921 agctggcttg aaatgataac caccgcatta caacaaaaaa gagaaaataa caggagtgat
     4981 gactatgagt cgtttagtcg tagtatctaa ccggattgca ccaccagacg agcacgccgc
     5041 cagtgccggt ggccttgccg ttggcatact gggggcactg aaagccgcag gtggactgtg
     5101 gtttggctgg agcggtgaaa cagggaatga ggatcagccg ctaaaaaagg tgaaaaaggg
     5161 taacattacg tgggcctctt ttaacctcag cgaacaggac cttgacgaat actacaacaa
     5221 attctccaat gccgttctct ggcccgcttt tcattatcgg ctcgatctgg tgcaatttca
     5281 gcgtcctgcc tgggatggct atctacgcgt aaatgcgttg ctggcagata aattactgcc
     5341 gctgttgcaa gacgatgaca ttatctggat ccacgattat cacctgttgc cctttgcgca
     5401 tgaattacgc aaacggggag tgaataatcg cattggtttc tttctgcata ttcctttccc
     5461 gacgccggaa atcttcaacg cgctgccgac atatgacacc ttgcttgaac agctttgtga
     5521 ttatgatttg ctgggtttcc agacggagaa cgatcgtctg gcgttcctgg attgcctttc
     5581 taacctgacc cgcgtcacga cacgtagcgc aaaaagccat acggcctgcg gcaaagcgtt
     5641 tcgaacggaa gtttacccga tcggcattga accgaaagaa atagcgaaac aggctgccgg
     5701 accactgccg ccaaaactgg cgcaacttaa agcggaactg aaaaacgtac aaaatatctt
     5761 ttctgtcgaa cggctggatt attccaaagg tttgccggag cgttttctcg cctatgaagc
     5821 gttgctggaa aaatatccgc agcatcatgg taaaattcgt tatacccaga ttgcaccaac
     5881 gtcgcgtggt gatgtgcaag cctatcagga tattcgtcat cagctcgaaa atgaagctgg
     5941 acgaattaat ggtaaatacg ggcaattagg ctggacgccg ctttattatt tgaatcagca
     6001 ttttgaccgt aaattactga tgaaaatatt ccgctactct gacgtgggct tagtgacgcc
     6061 actgcgtgac gggatgaacc tggtggcaaa agagtatgtt gccgctcagg acccagccaa
     6121 cccgggcgta cttgttcttt cgcaatttgc gggagcagca aacgagttaa cgtcggcgtt
     6181 aattgttaat ccctacgatc gtgacgaagt tgcagctgcg ctggatcgtg cattgactat
     6241 gtcgctggcg gaacgtattt cccgtcatgc agaaatgctg gacgttatcg tgaaaaacga
     6301 tattaaccac tggcaggagt gcttcattag cgacctaaag cagatagttc cgcgaagcgc
     6361 ggaaagccag cagcgcgata aagttgctac ctttcctaag cttgcgtagg agcggttaat
     6421 ctcccgtaag tggaactaac agcacatcga cctcacttga ggcaatcact cttttcgccg
     6481 agcaggatgc tcgcgaaaag aaactgtgat tgtgattacc gcaaatcacc aaatcgaaat
     6541 gatatttgcg acaaacttcc agaatatgtt cgcttaactc accgtaggca ataaacgtct
     6601 tgtcaacggg ataccccgca tcctgaatta acttatcaag aaagctttgc gtttcttcct
     6661 gcatcacgct gcgtaaatct tcaagcatcg gcgcagctaa ttgattgtac atttccgggt
     6721 cggaagcgag agtaattaaa ctgatgtgtc cctttactgg cctggcgata gataccgctt
     6781 ttgccagcag ttgctgactt tccggtgtaa ccgcaacagc gacaagaata ttgctatagc
     6841 tcataacctg ttccttattc tgtgaacttc aggtgacatt aaagctttac tcctaatgga
     6901 gaaacgacgc aatcccaact cagtcaaaca
//
LOCUS       DAZFLD010000216         6909 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.217, whole genome shotgun sequence.
ACCESSION   DAZFLD010000216 DAZFLD010000000
VERSION     DAZFLD010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6909)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6909)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6909
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.217"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            26..523
                     /gene="ftnA"
                     /locus_tag="ACHKBL_004113"
     CDS             26..523
                     /gene="ftnA"
                     /locus_tag="ACHKBL_004113"
                     /EC_number="1.16.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin"
                     /protein_id="HFO5820908.1"
                     /translation="MLKPEMIEKLNEQMNLELYSSLLYQQMSAWCSYHTFEGAAAFLR
                     RHAQEEMTHMQRLFDYLTDTGNLPRINTVESPFAEYSSLDELFQETYKHEQLITQKIN
                     ELAHAAMTNQDYPTFNFLQWYVSEQHEEEKLFKSIIDKLSLAGKSGEGLYFIDKELST
                     LDTQN"
     gene            complement(561..800)
                     /locus_tag="ACHKBL_004114"
     CDS             complement(561..800)
                     /locus_tag="ACHKBL_004114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecH family metal-binding protein"
                     /protein_id="HFO5820909.1"
                     /translation="MDSIHGHEVLNMMIESGEQYTHASLEAAIKARFGEQARFHTCSA
                     EGMTAGELVAFLAAKGKFIPSEEGFSTDQSKICRH"
     gene            990..2201
                     /gene="tyrP"
                     /locus_tag="ACHKBL_004115"
     CDS             990..2201
                     /gene="tyrP"
                     /locus_tag="ACHKBL_004115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015173 - aromatic amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015801 - aromatic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter TyrP"
                     /protein_id="HFO5820910.1"
                     /translation="MKNRTLGSVFIVAGTTIGAGMLAMPLAAAGVGFSVTLILLIGLW
                     ALMCYTALLLLEVYQHVPADTGLGTLAKRYLGRYGQWLTGFSMMFLMYALTAAYISGA
                     GELLASSISDWTGISMSATAGVLLFTFVAGGVVCVGTSLVDLFNRFLFSAKIIFLVVM
                     LVLLLPHIHKVNLLTLPLQQGLALSAIPVIFTSFGFHGSVPSIVSYMDGNIRKLRWVF
                     IIGSAIPLVAYIFWQLATLGSIDSTTFMGLLANHAGLNGLLQALREMVASPHVELAVH
                     LFADLALATSFLGVALGLFDYLADLFQRSNTVGGRLQTGAITFLPPLAFALFYPRGFV
                     MALGYAGVALAVLALIIPSLLTWQSRKHNPQAGYRVKGGRPALVVVFLCGIAVIGVQF
                     LIAAGLLPEVG"
     gene            complement(2252..2917)
                     /gene="yecA"
                     /locus_tag="ACHKBL_004116"
     CDS             complement(2252..2917)
                     /gene="yecA"
                     /locus_tag="ACHKBL_004116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YecA"
                     /protein_id="HFO5820911.1"
                     /translation="MKTGPLNESELEWLDDILTKYNTDHAILDVAELDGLLTAVLSSP
                     QEIESAQWLVAVWGGADYVPRWASEKEMTRFMNLAFQHMADTAERLNEFPEQFEPLFG
                     LREVDGSELTIVEEWCFGYMRGVALSDWSTLPDSLKPALDAIALHGTEENFERVEKMS
                     PEAFEESVDAIRLAALDLHAYWMAHPQEKAVQQPIKAEEKPGRNDPCPCGSGKKFKQC
                     CLH"
     gene            complement(3113..3199)
                     /locus_tag="ACHKBL_004117"
     tRNA            complement(3113..3199)
                     /locus_tag="ACHKBL_004117"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3163..3165),aa:Leu,seq:taa)
     gene            complement(3212..3285)
                     /locus_tag="ACHKBL_004118"
     tRNA            complement(3212..3285)
                     /locus_tag="ACHKBL_004118"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3251..3253),aa:Cys,seq:gca)
     gene            complement(3340..3415)
                     /locus_tag="ACHKBL_004119"
     tRNA            complement(3340..3415)
                     /locus_tag="ACHKBL_004119"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3380..3382),aa:Gly,seq:gcc)
     gene            complement(3567..4115)
                     /gene="pgsA"
                     /locus_tag="ACHKBL_004120"
     CDS             complement(3567..4115)
                     /gene="pgsA"
                     /locus_tag="ACHKBL_004120"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HFO5820912.1"
                     /translation="MQFNIPTLLTLFRVILIPFFVLVFYLPVTWSPFAAALIFCVAAV
                     TDWFDGFLARRWNQSTRFGAFLDPVADKVLVAIAMVLVTEHYHSWWVTLPAATMIARE
                     IIISALREWMAELGKRSSVAVSWIGKVKTTAQMVALAWLLWRPNIWVEYAGIALFFVA
                     AVLTLWSMLQYLSAARADLLDQ"
     gene            complement(4172..6004)
                     /gene="uvrC"
                     /locus_tag="ACHKBL_004121"
     CDS             complement(4172..6004)
                     /gene="uvrC"
                     /locus_tag="ACHKBL_004121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HFO5820913.1"
                     /translation="MSDQFDAKAFLKTVTSQPGVYRMYDAGGTVIYVGKAKDLKKRLS
                     SYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLLEHNYIKLYQPRYNVLLRDDKSY
                     PFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
                     NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETAS
                     QNLEFEEAARIRDQIQAVRRVTEKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKV
                     LGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLDFNLSDKTLLADSLS
                     ELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEV
                     KRMECFDISHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRY
                     GKAIDESKIPDVILIDGGKGQLAQAKNVFAELDVSWDKNHPLLLGVAKGADRKAGLET
                     LFFEPEGEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLETIEGVGP
                     KRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH"
     gene            complement(6001..6657)
                     /gene="uvrY"
                     /locus_tag="ACHKBL_004122"
     CDS             complement(6001..6657)
                     /gene="uvrY"
                     /locus_tag="ACHKBL_004122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HFO5820914.1"
                     /translation="MINVLLVDDHELVRAGIRRILEDIKGIKVVGEASCGEDAVKWCR
                     ANAVDVVLMDMSMPGIGGLEATRKIARSTADVKIIMLTVHTENPLPAKVMQAGAAGYL
                     SKGAAPQEVVSAIRSVYSGQRYIASDIAQQMALSQIEPEKTESPFASLSERELQIMLM
                     ITKGQKVNEISEQLNLSPKTVNSYRYRMFSKLNIHGDVELTHLAIRHGLCNAETLSSQ
                     "
BASE COUNT         1665 a         1794 c         1640 g         1810 t
ORIGIN      
        1 aatataacct ttgtggagca ctatcatgct gaaaccagaa atgattgaaa aacttaatga
       61 gcagatgaac ctggaactgt actcttcact gctttatcag caaatgagcg cctggtgcag
      121 ctatcatacc ttcgaaggtg ctgccgcgtt cctgcgccgt cacgcccagg aagagatgac
      181 gcatatgcag cgtctgtttg attacctgac tgataccggc aacttaccgc gtattaatac
      241 cgttgaatct ccgtttgctg aatattcctc acttgatgaa ttattccagg aaacctataa
      301 acacgaacaa ttaatcacgc agaaaattaa cgaactggct catgctgcaa tgaccaatca
      361 ggactaccca acatttaatt tcctgcaatg gtatgtttct gagcagcatg aagaagagaa
      421 actgttcaaa tcgattattg ataaattaag cctggcaggc aaaagtggcg aaggtctgta
      481 ttttatcgac aaagaactct ctaccctcga cacacaaaac taatgctctc cggcggcagg
      541 ttttctgccg ccgtttcaaa tcagtgacgg caaatcttac tctgatcggt tgaaaaacct
      601 tcttctgaag gtataaattt gccttttgct gccagaaacg ccaccaactc tcccgcagtc
      661 atcccttctg ccgagcaggt gtgaaaacgt gcctgttcac caaaacgcgc tttaattgct
      721 gcttccagac tggcatgcgt atattgctcg cctgattcaa tcatcatatt taacacttcg
      781 tgaccgtgaa tagagtccat cgttcctcct caaaaaaagc ctagcgtagc gattgccgct
      841 tatgaagact ttgcgccagc gcagaactga acgcttttat tgtacattta tatttacatc
      901 atatgtaacg ccggtttgac gaatcagccg ttatgcctta acctgcgccg cagatatcac
      961 tcataaagat cgtcaggaca gaagaaagcg tgaaaaacag aactctggga agtgttttta
     1021 tcgtggcggg aaccacaatt ggcgcaggca tgctggcaat gccgctggct gcggcaggtg
     1081 ttggttttag cgtcacgtta atcttgttaa ttgggctttg ggcgttgatg tgctacacgg
     1141 cgctattact gctggaggtg taccagcatg tcccggcaga taccggtctg ggcacgctgg
     1201 caaaacgcta tctgggacgc tacggtcagt ggctgacggg cttcagtatg atgttcttaa
     1261 tgtatgctct gactgcggca tacatcagcg gtgccggtga attgttggcc tccagcatca
     1321 gcgactggac aggtatttca atgtcggcaa ccgctggcgt gctgttgttc acttttgttg
     1381 ccggtggcgt ggtttgtgtc ggaacttcgc tggtcgattt atttaaccgt tttctgttca
     1441 gcgccaaaat tatttttctg gtggtgatgc tggtattgct gctaccgcat attcacaaag
     1501 tgaatctttt aaccctgccg ttgcaacagg gactggcact gtctgcaatc ccggtgattt
     1561 ttacgtcgtt tggttttcac ggtagcgtgc cgagtattgt cagctatatg gatggcaaca
     1621 ttcgtaagct acgctgggtg tttataatcg gtagtgcgat ccccctggtg gcatatattt
     1681 tctggcagct ggcgaccctt ggcagcattg attcaacaac ctttatggga ttgctggcta
     1741 atcatgccgg attaaacggg ctgttacagg cgttacgcga aatggtggcc tctccgcatg
     1801 ttgagctggc agtgcattta tttgctgatt tagccctcgc cacgtccttc ctcggcgtag
     1861 cgttaggctt atttgattat ctggcagatt tgtttcagcg ttcaaatacc gttggtggac
     1921 gattgcaaac tggtgcaatt acctttctgc cgccactggc gtttgcactg ttttatccac
     1981 gaggatttgt gatggcgctg ggttacgccg gtgtggcgct ggcggtactg gcattgatta
     2041 tcccttcgct gttgacctgg caaagcagga agcacaatcc tcaggcgggt taccgggtga
     2101 aaggtggtcg tccggcgctg gtggtggtgt ttctctgtgg tattgctgtg attggcgtgc
     2161 aatttttgat tgcggcaggc ttgttaccag aagtggggtg attagattct tattgggtgc
     2221 cagaattaac gctgacaccc aatttgacct cttaatgcag gcagcactgc ttaaatttct
     2281 taccactacc gcacggacaa ggatcgttac gccccggttt ctcttctgct ttgataggtt
     2341 gctgaacagc tttttcctgc ggatgcgcca tccagtatgc atgtaaatca agcgccgcca
     2401 gtcgaatagc atctacgctc tcttcaaacg cttctggcga catcttctct acccgctcga
     2461 agttttcctc agtaccgtgc agcgcaatcg catccagcgc tggttttaac gaatcgggca
     2521 acgttgacca gtcagaaagt gccacgcccc gcatatagcc aaagcaccac tcctcaacaa
     2581 tcgtcagctc gctgccatcg acttctcgca agccgaataa cggctcaaac tgctccggga
     2641 attcgttcag acgctctgcg gtatcagcca tatgttgaaa agccaggttc ataaagcgcg
     2701 tcatctcttt ctctgacgcc cagcgcggca catagtcagc cccaccccac acggcaacca
     2761 gccactgtgc cgattcaatc tcttgcggag aactcaacac cgccgtcaat aaaccgtcca
     2821 gctccgccac atcaagaatg gcgtggtcag tgttgtattt ggtcagaata tcgtccagcc
     2881 attccaactc actttcgttt aacggtcccg ttttcatacg cttttccttg tggatctcaa
     2941 ctcgccagca cctatcttac atgccggtcc gtatcagaga tactttttga gtggctttgc
     3001 cggtgattaa aaattaagga gggtgtaacg acaagttgca ggcacaaaaa aaccacccga
     3061 aggtggtttc acgacactgc ttattgcttt gattttattc ttatctttcc catggtaccc
     3121 ggagcgggac ttgaacccgc acagcgcgaa cgccgaggga ttttaaatcc cttgtgtcta
     3181 ccgattccac catccgggct cgggaagaaa gtggaggcgc gttccggagt cgaaccggac
     3241 tagacggatt tgcaatccgc tacataaccg ctttgttaac gcgccaaatt cttcaggcct
     3301 ttcagccaga catccgcttg acgccgatgt cttttaaact ggagcgggaa acgagactcg
     3361 aactcgcgac cccgaccttg gcaaggtcgt gctctaccaa ctgagctatt cccgcattca
     3421 tcaagcaatc agttaatcac ttgattttat tatcgtctgg caatcagtgc cgccgttcga
     3481 tgcgttgcat tctacttacc tggcgcgatg agtcaacgat atttttcacc acttttgatc
     3541 gtttgctgaa aattgcgccg aaacgatcac tgatcaagca aatctgcacg cgcagcgctc
     3601 aaatattgca acattgacca cagagtcagt accgcagcca caaagaaaag tgcaataccg
     3661 gcgtactcaa cccaaatgtt cggacgccac agcagccagg ccagcgccac catctgggca
     3721 gtggttttca ctttcccaat ccaggagaca gccacgctac tgcgtttacc caactccgcc
     3781 atccattcgc gtaacgcaga aataataatt tcacgggcga tcatcgttgc tgccggtaag
     3841 gtcacccacc agctgtgata atgctcggtt accagcacca tggcgatagc cacgagaact
     3901 ttatctgcta cagggtcaag gaaagcacca aaccgggtac tctggttcca gcggcgtgcc
     3961 agaaaaccat cgaaccagtc agtcaccgcc gcgacgcaga aaatgagcgc ggcggcaaac
     4021 ggcgaccagg tgacaggcag ataaaagacc aatacaaaga atgggataag gatgacacgg
     4081 aacagtgtaa gcaacgtagg gatattaaat tgcataatga cgggtaacta tctgttgtca
     4141 gtaagattac ccctatgttg ctacagagac atcaatgttt caacgaccag aagatctttt
     4201 ctgccagacc ttgcgaaata cccggcactt ttgcaatttc ctcgacgctg gcgttacgta
     4261 aaccttgcaa accgcccata tatttcaaca acatttgccg acgttttggc ccgacgcctt
     4321 caatggtttc cagggaactg gtatttttga ccttcgcccg ttttttacgg tgcccgccaa
     4381 tcgcgtgatc gtgtgattca tcgcgaatat gctggataac atgcagtgcg ggagaatctg
     4441 gcggcaaact gaatccctca ccttccggct caaagaacag cgtttccagc ccagccttac
     4501 gatctgctcc tttggcaacg cctagtagca gcggatgatt tttatcccat gagacatcca
     4561 gttcggcgaa aacatttttc gcctgcgcaa gttggccttt gccgccgtcg ataaggatca
     4621 catccgggat cttactctcg tcgatggctt taccataacg ccgacgcagc acctgattca
     4681 tcgccgcata atcatcgccc ggcgtgatgc cagtaatgtt atagcgtcga tactccgcac
     4741 gcagcgggcc gttagcatca aacaccacac aggaagcgac ggtttgttcc cccatggtat
     4801 ggctgatgtc aaagcattcc atccgcttca cttccggcaa tttcaacacg ctggcaagcg
     4861 cggtcagtcg ctggtgaacg gtagattgct gcgaaagttt gctggttaag gccgtcgccg
     4921 cattggtgcg cgcgagtttc agataacgcg cccgatcgcc gcgaggtttg gtttgaacat
     4981 taatcttgcg tcccgccagt tctgaaaggg aatcggcgag cagcgtttta tcgctaagat
     5041 taaaatcgag caggatctca cccggtaagg tgcgcatctg gctgccttgt aaatagaact
     5101 gacctacgaa ggtttccacc acctcgctca gttccgtacc gccaggcact ttcgggaaat
     5161 agctgcggct gccgagcact ttaccctgac gaatgaacaa tacgtggaca caagccatgc
     5221 ccgcatcgaa cgccacacca ataacgtcga ggtcgtcgcc ggtattggac acaaactgtt
     5281 tttcggtgac gcgtcgcacc gcctgaattt ggtcgcgaat acgtgccgct tcttcaaact
     5341 ccagattctg gctggcagtt tccatacgac taatgagttg cgtaagcacc tgatcatctt
     5401 tgccagacaa aaacaggcgc acatactcga cctgctgagc gtattcttct tcactcacca
     5461 gtccttcaac gcacggtccc agacagcgcc ctatctgata ttgcagacac ggacgcgagc
     5521 gattgcgata aacactattt tcgcactggc gaatggggaa tatcttttgc agtagcgcca
     5581 gtgtttcacg tacggcatag ccattcggga acgggccgaa atattcacct ttagcatgct
     5641 tcgcaccacg atgcatcgcc agacgcggat gggtatcgcc actcaggaag ataaagggat
     5701 aagatttatc atcgcgtagc aaaacgttgt aacgcggctg atagagtttg atgtagttgt
     5761 gttccagcaa cagcgcttcg gtttctgtat gagtaaccgt tacatcaatt tgctggatct
     5821 gggcgaccag cgcttcggtt ttgcgcgaag cgaggttgct acggaaatag ctggaaagcc
     5881 gttttttcag gtctttcgct ttgccgacat agataaccgt accaccagca tcgtacatgc
     5941 gataaacgcc tggctggctg gttacggttt ttaaaaacgc ttttgcgtca aactgatcac
     6001 tcactgactt gataatgtct ccgcattaca cagaccatgg cgaattgcca ggtgagtcag
     6061 ctcaacatcg ccatgaatgt ttagtttact gaacatacga tagcggtagc tgttcaccgt
     6121 tttcggactg agattgagct gttctgagat ctcattgacc ttctggccct tggtgatcat
     6181 cagcataatc tgcaattcac gttcagacaa actggcaaat gggctttctg ttttttctgg
     6241 ttcgatctgg cttaacgcca tttgttgagc gatgtcagaa gcaatgtaac gctgccctga
     6301 atagacagaa cggatcgcac tcacaacttc ctgcggagcc gcgcctttgc tgaggtagcc
     6361 cgcagcaccg gcctgcatga ctttcgctgg taaagggttt tctgtatgga cggtaagcat
     6421 gatgatttta acatcagctg tggaacgcgc gattttacgc gtcgcctcaa ggccgccaat
     6481 gcccggcata ctcatgtcca ttagcaccac gtcaacggca tttgcccggc accatttaac
     6541 ggcgtcttca ccgcacgatg cctctccgac gacttttata ccctttatat cttccagaat
     6601 gcgtcgtatc cctgcgcgca ccagttcgtg gtcatcaaca agtagaacgt tgatcaaagg
     6661 aatatctcca gaaataggga taacgctact gatagttagt cattcgtata ttagcggttt
     6721 ttattgcaac tttgaaacgt taaaaatggt gggtattcga tttttctctc gtttttgtaa
     6781 attcgattgt ccacagttat ggtcacgccc gtcaccaacg gcttccggga tcgtttcact
     6841 gtaagccctg cgatgaaatg tgacaaaaat tgactttatt cagcaaaaat gaaaatcagc
     6901 ccgctgaat
//
LOCUS       DAZFLD010000217         6861 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.218, whole genome shotgun sequence.
ACCESSION   DAZFLD010000217 DAZFLD010000000
VERSION     DAZFLD010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6861)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6861)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6861
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.218"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(407..1297)
                     /gene="ybcH"
                     /locus_tag="ACHKBL_004123"
     CDS             complement(407..1297)
                     /gene="ybcH"
                     /locus_tag="ACHKBL_004123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 family protein"
                     /protein_id="HFO5820915.1"
                     /translation="MRKFIFVLLTLLLVSPFSFAMKGIIWQPQNRDSQVTDTQWQGLM
                     SQLRLQGFDTLVLQWTRYGDAFTQPEQRALLFKRAAAAQQAGLKLIVGLNADPEFFIH
                     QKQSSAALESYLNRLLAADLQQARLWSAVPGVTPDGWYISAEIDDLNWRSEAARQPLL
                     TWLNNAQRLISDVSAKPVYISSFFAGNMSPDGYRQLLEQVKATGVNVWVQDGSGVDKL
                     TAEQRERYLQASADCQSSAPASGIVYELFVAGKGKTFTAKPKPDAEIASLLAKRSSCG
                     KDTLYFSLRYLPVAQSILEY"
     gene            complement(1298..4270)
                     /gene="nfrA"
                     /locus_tag="ACHKBL_004124"
     CDS             complement(1298..4270)
                     /gene="nfrA"
                     /locus_tag="ACHKBL_004124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage adsorption protein NfrA"
                     /protein_id="HFO5820916.1"
                     /translation="MKENNLNRVIGWSGLLLTSLLSTSALADNIGTSAEELGLSDYRH
                     FVIYPRLDKALKAQKNNDEATAIREFEYIHQQVPDNIPLTLYLAEAYRHFGHDDRARL
                     LLEDQLKRHPGDARLERSLAAIPVEVKSVTTVEELLAQQKACDAAPTLRCRSEVGQNA
                     LRLAQLPVARAQLNDATFAASPEGKTLRTDLLQRAIYLKQWSQADTLYNEARQQNTLS
                     AAERRQWFDVLLAGQLDDRILALQSQGIFTDPQSYITYATALAYRGEKARLQHYLIEN
                     TPLFTTDAQEKSWLYLLSKYSGNPVQALANYTVQFADNRQYVVGATLPVLLKEGQYDA
                     AQKLLATLPANEMLEERYTVSVATRNKAEALRLARLLYQQEPANLTRLDQLTWQLMQN
                     EQSREAADLLLQRYPFQGDARVSQTLMARLASLLESHPYLATPAKVAILSKPLPLAEQ
                     RQWQSQLPGIADNCPAIVRLLGDMSPSYDAAAWNRLAKCYRDTLPGVALYAWLQAEQR
                     QPNAWQHRAVAYQAYQVEDYATALAAWQKISLHGMSNEDLLAAANTAQAAGNGAARDR
                     WLQQAEQRGLGNNALYWWLHAQRYIPGQPELALSDLTRSINIAPSANAYVARATIYRQ
                     RHNVPAAVSDLRAALELEPNNSNTQAALGYALWDSGDIAQSREMLEQAHKGLPDDPAL
                     IRQLAYVNQRLDDMPATQHYARLVIDDIDNQALITPLTPEQNQQRFNFRRLHEEVGRH
                     WTFSFDSSIGLRSGAMSTANNNVGGAAPRKSYRSYGQLEAEYRIGRNMLLEGDLLSVY
                     SRVFADTGENGVMMPVKNPMSGTGLRWKPLRDQIFFLAVEQQLPLNGQNGASDTMLRA
                     SASFFNGGKYSDEWHPNGSGWFAQNLYLDAAQYIRQDIQAWTADYRVSWHQKVANGQT
                     IEPYAHVQDNGYRDKGTQGAQLGGVGVRWNIWTGETHYDAWPHKVSLGIEYQHTFKAI
                     NQRNGERNNAFLTIGVHW"
     gene            complement(4257..6494)
                     /gene="nrfB"
                     /locus_tag="ACHKBL_004125"
     CDS             complement(4257..6494)
                     /gene="nrfB"
                     /locus_tag="ACHKBL_004125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-3',5'-guanylate-activated
                     glycosyltransferase NrfB"
                     /protein_id="HFO5820917.1"
                     /translation="MDWLLDVFATWLYGLKVIAITLAVIMFISGLDDFFIDVVYWVRR
                     IKRKLSVYRRYPRMSYRELYKPDEKPLAIMVPAWNETGVIGNMAELAATTLDYENYHI
                     FVGTYPNDPDTQRDVDEVCARFPNVHKVVCARPGPTSKADCLNNVLDAITQFERSANF
                     AFAGFILHDAEDVISPMELRLFNYLVERKDLIQIPVYPFEREWTHFTSMTYIDEFSEL
                     HGKDVPVREALAGQVPSAGVGTCFSRRAVTALLADGDGIAFDVQSLTEDYDIGFRLKE
                     KGMTEIFVRFPVVDEAKEREQRKFLQHARTSNMICVREYFPDTFSTAVRQKSRWIIGI
                     VFQGFKTHKWTSSLTLNYFLWRDRKGAISNFVSFLAMLVMIQLLLLLAYESLWPDAWH
                     FLSIFSGSAWLMTLLWLNFGLMVNRIVQRVIFVTGYYGLTQGLLSVLRLFWGNLINFM
                     ANWRALKQVLQHGDPRRVAWDKTTHDFPSVTGDTRSLRPLGQILLENQVITEEQLDTA
                     LRNRVEGLRLGGSMLMQGLISAEQLAQALAEQNGVAWESIDAWQIPSSLIAEMPASVA
                     LHYAVLPLRLENDELIAGSEDGIDPVSLAALTRKVGRKVRYVIVLRGQIVTGLRHWYA
                     RRRGHDPRAMLYNAVQHQWLTEQQAGEIWRQYVPHQFLFAEILTTLGHINRSAINVLL
                     LRHERSSLPLGKFLVTEGVISQETLDRVLTIQRELQVSMQSLLLKAGLNPEQVAQLES
                     ENEGE"
     gene            complement(6692..>6861)
                     /locus_tag="ACHKBL_004126"
     CDS             complement(6692..>6861)
                     /locus_tag="ACHKBL_004126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HFO5820918.1"
                     /translation="IRRGNAAELFSGLRHIAINILTNDKVFKAGLRRKLRKAAMDRNY
                     LASVLAGSGLS"
BASE COUNT         1614 a         1789 c         1787 g         1671 t
ORIGIN      
        1 gcatgaggtt tatcctcaat tgctgtgttt tttagtataa aaaagaggaa caaaactgag
       61 acacataagg cctcgcaatg gcttgcaagg ttttacatat tttgaggtgg tggaacgtgt
      121 gaacgcagag atggcgcggt aatttgttga tttaaaatgt cgttcctgga gtttctaagt
      181 cgtgggctac aggttcgaat cctgcagggc gagccatttc ctcgccattt atttgttccc
      241 ttttacaaat tacaccatca ctttgttgtc gcggttttgc taaataattg gaacacgtca
      301 gacgaataaa atgaaaacag aaattctgct aaacaattca tcttgccagg attttgatta
      361 ggaaagtgaa aaggaaagga gcaagctaag ttgctccctt taatcattaa tactcaagga
      421 tactctgcgc gacgggcaaa tagcgcagag agaaatagag cgtgtcttta ccgcaagagg
      481 aacgtttcgc taacagcgag gcgatctctg catccggttt cggtttcgct gtaaacgttt
      541 tacctttacc agcgacaaac agttcataaa caatgccgct tgctggggcg gaactttggc
      601 aatcggcgct ggcctgtaga taacgttcac gctgttcagc ggtcagttta tccacgccgc
      661 tgccatcctg tacccagaca ttaacaccgg ttgctttaac ttgttccagc agttggcgat
      721 agccatctgg cgacatgttt ccggcgaaaa aactactgat ataaaccggt tttgctgaaa
      781 catcgctaat caaccgctgc gcgttgttta accatgttag caaaggttga cgggcggctt
      841 cgctgcgcca gttcaggtcg tcaatttccg cgctgatgta ccagccatcc ggcgttacgc
      901 caggcacggc gctccataat ctggcttgct ggagatcggc agccagcagg cgattaagat
      961 agctttccag cgctgcggag gactgtttct ggtgtataaa aaattccgga tcggcgttca
     1021 gcccgacaat cagcttcagg ccagcctgtt gcgcagctgc ggcacgctta aacaataacg
     1081 cgcgctgttc tggctgggtg aatgcatcgc cgtaacgggt ccattgcaaa accagggtat
     1141 cgaagccttg caaacgtaac tgactcatca gcccctgcca ctgggtatcg gtaacctgac
     1201 tatcacggtt ttgtggttgc cagataatac ctttcatcgc aaaggaaaaa gggctgacca
     1261 aaagcagtgt caacaatacg aaaatgaact tacgcattta ccagtgcact ccaatggtga
     1321 gaaacgcgtt gttgcgctct ccgttacgtt gattaatcgc cttaaaggta tgttgatatt
     1381 cgatgccgag actgactttg tgcggccagg cgtcgtagtg cgtctcgccg gtccagatat
     1441 tccagcggac cccgacaccg ccaagctgcg cgccctgagt gcctttatca cgataaccgt
     1501 tgtcctgaac gtgagcataa ggctcaatag tctgcccgtt tgccaccttc tgatgccagc
     1561 tgactcgata atctgccgtc cacgcctgaa tatcctggcg gatatattgc gccgcatcga
     1621 ggtacaggtt ttgggcaaac cagcctgaac cgttcgggtg ccattcgtcg ctgtatttgc
     1681 cgccattaaa gaatgaggcg ctggcgcgca gcatggtatc ggatgcgcca ttttgtccgt
     1741 tcagcggcaa ctgctgttcg acggcgagga aaaagatctg atcgcgcagc ggcttccagc
     1801 gcagaccggt gccggacatc ggatttttca ccggcatcat caccccgttt tcaccggtat
     1861 cggcaaagac gcggctataa actgagagca ggtcgccttc cagcagcata ttgcgtccga
     1921 tgcggtactc ggcttccagt tgcccgtagc tacgatagct tttccttggc gctgcgccgc
     1981 cgacattatt gttagcggta ctcattgcgc cggaacgcaa gccaatagaa gaatcgaaac
     2041 tgaacgtcca gtggcgaccg acctcctcat gcagacggcg gaaattgaag cgttgctgat
     2101 tttgctctgg ggtcagtggg gttatcagcg cctgattatc aatgtcatca atcaccagtc
     2161 gggcgtagtg ctgcgtcgca ggcatgtcat ccagacgctg gttcacgtag gccagttgtc
     2221 ggataagtgc cggatcgtcc ggtagccctt tatgcgcctg ttcgagcatt tcccgcgact
     2281 gtgcgatatc accgctatcc cacaaggcgt aaccgagcgc tgcctgggtg ttgctattat
     2341 tcggttccag ttccagagcg gcgcgcaaat cactcaccgc cgccgggaca ttatgacgtt
     2401 ggcgataaat tgtcgcccgc gcaacgtaag cgttggcaga aggcgcaata ttgattgagc
     2461 gcgtgagatc gctcagtgcg agttccggct gaccaggaat gtaacgttgc gcatgcagcc
     2521 accagtagag ggcattgttt cccagcccac gttgttctgc ctgctgaagc cagcgatcgc
     2581 gagccgcacc atttcctgcc gcctgggcgg tattggcagc agcaagcaga tcctcattgc
     2641 tcatgccgtg aagactgatt ttctgccagg ccgccagtgc ggtggcgtag tcctcaacct
     2701 gatacgcctg ataggctacc gcacgatgtt gccaggcgtt cggttgtcgt tgttcggcct
     2761 gaagccatgc atacaacgcc acaccgggta gcgtgtcccg ataacacttt gccagacggt
     2821 tccaggcggc agcatcgtag gaaggcgaca tatcgcccag caagcgaact attgccgggc
     2881 aattatctgc aatacccggc aactgacttt gccactgacg ttgctccgcc agcggtaagg
     2941 gtttcgataa aatcgccacc ttcgccggag ttgccaggta aggatgactt tccagcagag
     3001 acgccagtcg cgccattaaa gtctggctga cacgcgcatc gccctggaaa ggatagcgtt
     3061 gcagcaataa atcggcagct tcacgtgact gctcgttctg catcagttgc caggtcagtt
     3121 gatccaggcg ggtaagattt gccggttctt gctgatacag caatcgtgcc agacgcagag
     3181 cttcagcctt gttacgggtc gccacgctaa cggtataacg ctcctcaagc atttcattgg
     3241 cggggagggt ggcgagcagt ttttgcgctg cgtcgtactg accttctttt aacagcaccg
     3301 gtagcgtcgc gccaacaaca tactggcggt tgtcggcaaa ctgtaccgta taattcgcca
     3361 acgcctgaac ggggttgccg ctgtatttag ataacagata gagccaactt ttctcttgtg
     3421 cgtccgtggt aaatagcggc gtattttcaa tgagataatg ctggaggcgt gctttttcgc
     3481 cacgataagc cagcgcggtc gcgtaagtaa tatatgactg aggatcggtg aagatcccct
     3541 gtgattgcag cgccaggatc cgatcgtcca gctgcccggc aagaagcaca tcaaaccact
     3601 gacggcgttc tgccgcgctt aatgtgttct gctggcgtgc ttcattgtat agcgtatctg
     3661 cctgggacca ttgtttcagg tagattgccc gttgcagcag atcggttcgc agcgtttttc
     3721 cttccggcga tgcagcaaac gtcgcatcgt tcagttgcgc tctggcgaca ggtaattgtg
     3781 ccagccgcag ggcattctgc ccgacttcac tgcgacaacg cagggtcggc gcagcatcgc
     3841 acgctttttg ctgggcaagc agttcttcaa cagtcgtaac gcttttcact tcaactggaa
     3901 tagccgccag actgcgctca agtcgggcat ctcctgggtg acgtttcagt tgatcctcaa
     3961 gcaacagccg cgcccggtca tcatgaccaa aatggcgata ggcttccgca aggtataaag
     4021 tcagcggaat attatccggc acctgttggt gtatatattc aaattcgcgg atggcggttg
     4081 cttcgtcgtt atttttctgt gccttcagcg ccttatcgag acggggataa ataacaaaat
     4141 ggcgataatc gctcagcccc agctcttctg cgctggtgcc gatattgtct gcgagtgcgc
     4201 tggtgctcaa taaagacgtc agcagtaaac cagaccatcc gatgacgcga ttaaggttat
     4261 tctccttcat tttcggactc cagttgcgca acctgttctg ggtttaaacc tgctttgagt
     4321 aatagtgatt gcatcgaaac ttgtaattcg cgttgaattg tcaggacgcg atccaacgtt
     4381 tcctggctga taacgccttc ggtgaccaaa aacttgccga gcggcagaga actgcgttca
     4441 tggcgcaata acaacacgtt aattgctgaa cgattaatat gaccgagcgt ggtcagtatt
     4501 tcggcgaaca ggaactgatg cggcacatat tgccgccaga tttcaccggc ctgctgttcc
     4561 gtgagccact gatgctgaac cgcattgtac aacattgccc gcgggtcgtg accgcgtcgg
     4621 cgtgcatacc agtgacgtaa ccccgtgaca atttgtccgc gcagaacaat gacgtaacgc
     4681 actttgcgtc cgactttacg cgtcagggct gccagcgaaa ccgggtcaat accatcttca
     4741 ctgccggcaa ttaactcatc attttccaga cgcagcggca gcactgcata atgcagcgcc
     4801 acggaagccg gcatttcggc aatcagtgat gaagggatct gccaggcgtc gatcgactcc
     4861 cacgccacgc cgttttgctc tgccagcgcc tgtgccagct gttcggcgct aatcagcccc
     4921 tgcatcagca ttgaaccgcc caggcgtaga ccttcgacgc gattacgcag tgctgtatcg
     4981 agttgttctt cagtgatgac ctgattttcc agcaggattt ggcctaacgg gcgcaacgag
     5041 cgggtatcgc cagtcacgct ggggaagtca tgcgttgttt tatcccacgc gacgcgacgt
     5101 ggatcgccgt gttgaagtac ctgttttaac gcgcgccagt tggccatgaa gttaatcagg
     5161 ttgccccaga aaagacgcag gacagatagc agcccctgcg tcagaccgta gtagccagtg
     5221 acaaaaatca cccgctgcac gatgcggtta accatcaaac caaagtttag ccacagcagg
     5281 gtcattaacc atgcgctgcc gctgaaaata gaaaggaaat gccaggcatc gggccacaaa
     5341 ctttcatacg ccagtaacag caaaagctgg atcatcacca gcatcgcgag gaagctgaca
     5401 aagttactga ttgccccttt gcggtcgcgc cagagaaagt agttcagcgt caggctggag
     5461 gtccatttgt gggttttaaa gccctggaaa acaatgccga tgatccagcg agatttttgt
     5521 cgaaccgcag tcgaaaaggt atcggggaaa tattcgcgca cgcagatcat gtttgacgtc
     5581 cgcgcgtgct gtaaaaattt acgctgctcg cgttctttgg cttcgtccac caccggaaaa
     5641 cggacaaaaa tttccgtcat acctttttct ttcaggcgga aaccaatgtc gtaatcttca
     5701 gtcagactct gcacgtcgaa agcaataccg tcaccgtcag ctaacagtgc ggtcacggcg
     5761 cggcggctga aacaggtgcc gacgcctgcg ctgggcacct gtccggcgag ggcttcacgc
     5821 accggaacat ctttgccatg cagttctgaa aactcatcaa tgtaggtcat gctggtgaag
     5881 tgcgtccatt cgcgttcgaa cggatacact gggatctgaa tcagatcttt acgctcgacc
     5941 agatagttga acagacgcaa ttccatcggt gaaatcacat cttcggcgtc atgcagaata
     6001 aaaccagcaa aagcgaaatt ggcgctacgt tcaaattggg tgatggcgtc cagcacgttg
     6061 ttcagacagt cggctttgct ggtggggcca ggacgcgcgc agactacctt atgcacgttc
     6121 gggaagcgag cgcacacttc gtcaacatca cgctgagtat cggggtcgtt ggggtaggtg
     6181 ccaacaaaga tatgatagtt ttcgtagtcg agcgtggtcg ccgccagctc ggccatattg
     6241 ccgatgacgc ccgtttcatt ccacgccgga accataatcg ctaacggttt ttcatctggt
     6301 ttatacagtt cgcggtaact cattcgcggg tagcggcgat aaacactcaa cttgcgttta
     6361 atgcggcgta cccagtagac gacatcaata aaaaaatcgt ccagcccgct gatgaacatg
     6421 atgaccgcta acgttatcgc gattactttt aagccgtaga gccaggtagc aaaaacatca
     6481 agaagccagt ccacacaaaa accttacatt aacgctggtt atgtttaggg tggcgtatat
     6541 taaggttttt tatgaattgt gacagctttt taccattaat aggtatgact attgcggcac
     6601 gttattttta cactggttat aaaagttgcc gtttgcgtag aatgtagaca aaagcgtggt
     6661 gtcaaggagg ggaggttaac gcagggaaag attacgaaag cccgctccca gcaaggactg
     6721 acgcgagata gtttctgtcc atggctgctt ttcgcaactt acgtcttaac cctgccttga
     6781 ataccttatc attcgtcaaa atattaatag cgatgtgccg tagccctgaa aataattctg
     6841 ctgcatttcc tcttcttatt t
//
LOCUS       DAZFLD010000218         6765 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.219, whole genome shotgun sequence.
ACCESSION   DAZFLD010000218 DAZFLD010000000
VERSION     DAZFLD010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6765)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6765
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.219"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(15..326)
                     /gene="yqfB"
                     /locus_tag="ACHKBL_004127"
     CDS             complement(15..326)
                     /gene="yqfB"
                     /locus_tag="ACHKBL_004127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(4)-acetylcytidine aminohydrolase"
                     /protein_id="HFO5820919.1"
                     /translation="MQPNDITFFQRFQDDILAGRKTITIRDESESHFKTGDVLRVGRF
                     EDDGYFCTIEVTATSTVTLDTLTEKHAEQENMTLTDLKKVIADIYPGQTQFYVIEFKC
                     L"
     gene            371..1804
                     /gene="bglA"
                     /locus_tag="ACHKBL_004128"
     CDS             371..1804
                     /gene="bglA"
                     /locus_tag="ACHKBL_004128"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglA"
                     /protein_id="HFO5820920.1"
                     /translation="MKKLTLPKDFLWGGAVAAHQVEGGWNKGGKGPSICDVLTGGAHG
                     VPREITKEVLPGKYYPNHEAVDFYGHYKEDIKLFAEMGFKCFRTSIAWTRIFPKGDEA
                     QPNEEGLKFYDDMFDELLKYNIEPVITLSHFEMPLHLVQQYGSWTNRKVVDFFVRFAE
                     VVFERYKHKVKYWMTFNEINNQRNWRAPLFGYCCSGVVYTEHENPEETMYQVLHHQFV
                     ASALAVKAARRINPEMKVGCMLAMVPLYPYSCNPDDVMFAQESMRERYVFTDVQLRGY
                     YPSYVLHEWERRGFNIKMEDGDLDVLREGTCDYLGFSYYMTNAVKAEGGTGDAISGFE
                     GSVPNPYVKASDWGWQIDPVGLRYALCELYERYQKPLFIVENGFGAYDKVEEDGSIND
                     DYRIDYLRAHIEEMKKAVTYDGVDLMGYTPWGCIDCVSFTTGQYSKRYGFIYVNKHDD
                     GTGDMSRSRKKSFNWYKEVIASNGEKL"
     gene            complement(1968..4841)
                     /gene="gcvP"
                     /locus_tag="ACHKBL_004129"
     CDS             complement(1968..4841)
                     /gene="gcvP"
                     /locus_tag="ACHKBL_004129"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HFO5820921.1"
                     /translation="MTQTLSQLENSGAFIERHIGPDAAQQQEMLNAVGAQSLNALTGQ
                     IVPKDIQLATPPQVGAPATEYAALAELKAIASRNKRFTSYIGMGYTAVQLPPVILRNM
                     LENPGWYTAYTPYQPEVSQGRLEALLNFQQVTLDLTGLDMASASLLDEATAAAEAMAM
                     AKRVSKLKNANRFFVASDVHPQTLDVVRTRAETFGFEVIVDDAQKVLDHQDVFGVLLQ
                     QVGTTGEIHDYTALISELKSRKIVVSVAADIMALVLLTAPGKQGADIVFGSAQRFGVP
                     MGYGGPHAAFFAAKDEYKRSMPGRIIGVSKDAAGNTALRMAMQTREQHIRREKANSNI
                     CTSQVLLANIASLYAVYHGPVGLKRIANRIHRLTDILAAGLQQKGLKLRHAHYFDTLC
                     VEVADKAGVLARAEAAEINLRSDILNAVGITLDETTTRENVMQLFSVLLGDNHGLDID
                     TLDKDVAHDSRSIQPAMLRDDEILTHPVFNRYHSETEMMRYMHSLERKDLALNQAMIP
                     LGSCTMKLNAAAEMIPITWPEFAELHPFCPPEQAEGYQQMIAQLADWLVKLTGYDAVC
                     MQPNSGAQGEYAGLLAIRHYHESRNEGHRDICLIPASAHGTNPASAHMAGMQVVVVAC
                     DKNGNIDLTDLRAKAEQAGDNLSCIMVTYPSTHGVYEETIREVCEVVHQFGGQVYLDG
                     ANMNAQVGITSPGFIGADVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFVPGHSVV
                     QIEGMLTRQGAVSAAPFGSASILPISWMYIRMMGAEGLKKASQVAILNANYIASRLQD
                     AFPVLYTGRDGRVAHECILDIRPLKEETGISELDIAKRLIDYGFHAPTMSFPVAGTLM
                     VEPTESESKVELDRFIDAMLAIRAEIDQVKAGVWPLEDNPLVNAPHIQSELVAEWAHP
                     YSREVAVFPAGVADKYWPTVKRLDDVYGDRNLFCSCVPISEYQ"
     gene            complement(4960..5349)
                     /gene="gcvH"
                     /locus_tag="ACHKBL_004130"
     CDS             complement(4960..5349)
                     /gene="gcvH"
                     /locus_tag="ACHKBL_004130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005960 - glycine cleavage complex
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HFO5820922.1"
                     /translation="MSNVPAELKYSKEHEWLRKEAGGTYTVGITEHAQELLGDMVFVD
                     LPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDALSDSPELVNSEPYAGGWI
                     FKIKASDESELESLLDATAYEALLEDE"
     gene            complement(5373..6467)
                     /gene="gcvT"
                     /locus_tag="ACHKBL_004131"
     CDS             complement(5373..6467)
                     /gene="gcvT"
                     /locus_tag="ACHKBL_004131"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HFO5820923.1"
                     /translation="MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHAVRTDA
                     GMFDVSHMTIVDLRGSRTREFLRYLLANDVAKLTKSGKALYSGMLNASGGVIDDLIVY
                     YFTEDFFRLVVNSATREKDLSWITQHAEPFGIEITVRDDLSMIAVQGPNAQAKAATLF
                     NDAQRQAVEGMKPFFGVQAGDLFIATTGYTGEAGYEIALPNEKAADFWRALVEAGVKP
                     CGLGARDTLRLEAGMNLYSQEMDETISPLAANMGWTIAWEPADRDFIGREALEVQREH
                     GTEKLVGLVMTEKGVLRNELPVRFTDAQGNQHEGIITSGTFSPTLGYSIALARVPEGI
                     GETAIVQIRNREMPVKVTKPVFVRNGKAVA"
BASE COUNT         1592 a         1805 c         1765 g         1603 t
ORIGIN      
        1 ggcgtgagtt caatttaaag acatttaaat tcaatcacat aaaactgtgt ctggccggga
       61 tagatatcgg caatgacctt tttcagatca gtcagggtca tattttcctg ctctgcatgt
      121 ttttccgtca gtgtatccag cgttacggtt gaggttgcgg tgacttcaat cgtgcaaaaa
      181 taaccgtcgt cttcaaaacg cccgacacga agcacatcac ccgttttgaa gtgcgattca
      241 gactcgtcgc ggatggtgat ggttttacgc ccagccagaa tgtcatcctg gaaacgttga
      301 aaaaaagtga tgtcgtttgg ctgcatggta ctatttcctg taaaaattga ctcatctgga
      361 gcctatgata gtgaaaaaac tcaccttacc gaaagatttc ttatggggcg gcgcagttgc
      421 cgctcatcag gtcgaaggcg gctggaacaa aggcggcaaa gggccgagca tttgtgacgt
      481 tctgaccggt ggcgcacacg gcgtgccgcg cgaaatcacc aaagaagtgt tgccaggaaa
      541 atactatccg aaccatgaag ccgttgattt ttatggtcat tacaaggaag acatcaagct
      601 atttgccgaa atgggcttca aatgttttcg tacatccatc gcctggacgc gcatttttcc
      661 aaaaggcgat gaagctcagc caaacgaaga agggctgaag ttctacgatg atatgttcga
      721 tgaactgctg aaatacaaca tcgaaccggt gatcaccctc tcccactttg aaatgccgct
      781 gcatctggtg cagcaatacg gtagctggac caaccgtaaa gtggttgatt tctttgtccg
      841 tttcgcagaa gtggtatttg aacgctataa gcataaagtc aaatactgga tgaccttcaa
      901 cgaaattaac aaccagcgta actggcgtgc accgctgttc ggttactgct gctccggcgt
      961 ggtgtatacc gagcatgaaa acccggaaga gacgatgtac caggtgctgc atcaccagtt
     1021 tgtcgccagc gccctggcgg tgaaagccgc gcgtcgcatt aacccggaaa tgaaagtcgg
     1081 ctgtatgctg gcgatggtgc cgctttaccc ttactcctgt aatccggacg atgtgatgtt
     1141 cgcccaggag tcgatgcgcg aacgctacgt ctttaccgat gtgcaactgc gtggctatta
     1201 cccgtcctat gtgttgcacg agtgggagcg tcgcggattt aacatcaaaa tggaagacgg
     1261 cgatctcgat gtgctgcgcg aaggcacctg cgattatctc ggtttcagct attacatgac
     1321 caacgcggtg aaggccgaag gcggcaccgg cgatgcgatc tctggttttg aaggcagcgt
     1381 accaaacccg tatgttaaag cgtctgactg gggctggcag attgatcctg tgggtctgcg
     1441 ctatgcactt tgcgaactgt atgagcgtta tcagaagccg ctgtttattg tcgaaaacgg
     1501 ttttggcgct tatgacaaag tggaagaaga tggcagcatc aacgatgact accgcattga
     1561 ctacctgcgc gcccatattg aagagatgaa aaaagcggtg acttacgatg gcgtggatct
     1621 gatgggctac acaccgtggg gctgcatcga ttgcgtgtcg ttcactaccg ggcagtacag
     1681 caaacgctac ggctttatct atgtgaataa acatgacgac ggtactggcg atatgtcgcg
     1741 ttcacgtaag aagagcttta actggtacaa agaagtgatt gccagcaacg gcgagaagct
     1801 ttaattaccc gatgtgcctg atgcgccccg ctcatcaggc ctggcgctcc tgtcatatat
     1861 tgaactccag gagtttatat ttacgcttca tccgacacac aaagcgcact ttattaacaa
     1921 gctaaaaagg cgccgaagcg cctttagaag atagtcgaat cagtgaatta ctggtattcg
     1981 ctaatcggta cgcaggagca gaacaggtta cggtcgccgt aaacatcatc cagacgtttc
     2041 actgtcggcc agtatttgtc tgccacgcct gccgggaata ccgcaacttc acggctgtac
     2101 ggatgcgccc actcggcgac cagttcgctc tgaatgtgcg gcgcgttcac cagcgggtta
     2161 tcttccagcg gccagacacc ggctttcacc tggtcaattt ctgcgcggat agccagcatc
     2221 gcgtcgataa agcgatccag ttccactttg ctttcagatt cagtcggttc aaccatcagc
     2281 gtacccgcca ccgggaacga catggtcggc gcgtggaaac cgtagtcgat cagacgcttg
     2341 gcaatatcca gttcgctgat gccggtttct tctttcagcg ggcgaatatc gagaatacat
     2401 tcgtgcgcca cgcgaccgtc gcgaccggta tacagcaccg ggaaggcatc ctgcaggcgg
     2461 ctggcaatat agttggcgtt aagaattgcc acctggcttg cttttttcag cccttctgcg
     2521 cccatcatgc ggatgtacat ccagctgatt ggcagaatgg atgcgctacc gaacggtgcc
     2581 gcagaaaccg cgccctgacg ggttaacatg ccttcaattt gtaccacgct atgacctggc
     2641 acaaacggtg ccagatgcgc tttcacgccg atcggtccca taccagggcc gccgccaccg
     2701 tgcggaatgc agaaagtttt atgcaggtta aggtgcgaga cgtccgcgcc aataaagccc
     2761 ggtgaggtga tgccgacctg ggcgttcatg ttcgcgccat caaggtaaac ctgaccgccg
     2821 aactgatgca cgacttcaca cacttcacgg atcgtttctt catacacgcc gtgggtggaa
     2881 ggataggtca ccatgataca ggagaggtta tcgcccgcct gttccgcttt cgcgcgcaga
     2941 tcagtcagat cgatgttgcc gtttttatca cacgccacaa ccaccacctg cattcctgcc
     3001 atatgtgcag aagcggggtt agttccgtgc gcagaagccg ggatcaggca gatatcacga
     3061 tgcccttcgt tgcggctttc atgataatga cgaatcgcca gcaggcccgc gtattcgccc
     3121 tgtgcgccag agttcggctg catacaaacg gcgtcgtaac cggtcagttt caccagccag
     3181 tcagccagct gcgcaatcat ctgctgataa ccttcggcct gctccggcgg gcagaacggg
     3241 tgcagttcgg caaattccgg ccaggtgatt gggatcatct cggcggcggc gttcagtttc
     3301 atggtgcagg aacccagcgg gatcatcgcc tgattcagcg ccagatcttt acgctccagc
     3361 gagtgcatat agcgcatcat ttcggtttcg ctgtggtagc gattaaacac cggatgggtg
     3421 aggatttcgt cgtcgcgcag catcgcaggc tgaatagagc ggctgtcgtg agccacgtct
     3481 ttgtccagcg tgtcgatgtc caggccgtgg ttatccccca gcagtacgct gaaaagctgc
     3541 atcacgtttt cacgcgtggt ggtttcatca agggtgatcc caaccgcgtt caggatatcg
     3601 ctacgcaggt tgatttcagc cgcttcggca cgcgccagta cgcccgcctt gtctgccact
     3661 tccacacaca aagtgtcgaa atagtgcgca tgacgcagtt tcagaccttt ttgttgcagg
     3721 cccgccgcca ggatatcggt cagacggtgg atgcggttag cgatacgttt caggccaacc
     3781 gggccgtgat aaacggcata caggctggcg atgtttgcca gcagtacctg ggaagtacaa
     3841 atgttggagt tcgctttctc acggcgaata tgttgctcgc gagtctgcat cgccatgcgc
     3901 agagcggtat tgccagctgc atctttcgat acaccgataa tacggcccgg cattgagcgt
     3961 ttgtattcat ctttcgccgc aaagaatgct gcgtgtgggc caccgtagcc catcggcaca
     4021 ccgaagcgtt gcgccgaacc aaagacaata tccgcgccct gtttacccgg cgcagttaac
     4081 agcaccagcg ccattatatc ggcggcgaca ctgaccacaa ttttgcgtga tttcagttcg
     4141 ctgatgagcg cagtgtagtc gtggatttca ccggttgtgc caacctgctg taacagcacg
     4201 ccgaagacgt cctgatggtc gagcactttt tgcgcgtcat cgacaatcac ttcaaaacca
     4261 aaggtttcag cacgagtacg gaccacatcg agcgtttgcg gatgaacgtc agaagcaacg
     4321 aagaagcggt tggcattttt cagtttgctg acgcgtttcg ccatcgccat tgcttcggcg
     4381 gcagcagtgg cctcgtccag aagagaggca gaggccatat ccagcccggt caaatccagc
     4441 gttacctgct ggaagttgag cagtgcttca aggcggccct gggagacttc aggttgatac
     4501 ggagtatacg cggtatacca gcccggattt tccagcatgt tacgcagaat aaccggcggt
     4561 agctgcacgg cggtgtaacc catgccgatg taagacgtga agcgtttatt gcgactggca
     4621 atagccttga gttccgccag cgcggcgtat tcggtcgccg gtgcgccaac ctgcggcggg
     4681 gtcgcaagct gaatatcttt cggcacaatc tggccggtca gcgcgtttaa cgattgtgcg
     4741 ccaacggcat tcagcatttc ttgctgttgc gcggcgtccg gtccgatatg gcgttcaata
     4801 aaagcgccgc tgttttcaag ctggcttaac gtctgtgtca tgagcgatgg ttcctgaaac
     4861 gtgcagtgaa ttgtgaacct ctctccttgc gaagagagtg agggtgaggc gtaaaagttc
     4921 ctcaccctga tcctctcccg cagaagagga ataaagccgt tactcgtctt ctaacaatgc
     4981 ttcgtatgcg gtcgcatcca gcagtgattc cagttcgctt tcatcgctgg ctttgatttt
     5041 aaagatccag ccgcctgcat acggttcgct gttcaccagt tccggggaat cgctcagtgc
     5101 gtcgtttacc gccacgattt caccgcttac tggcgcataa atgtctgacg ccgcttttac
     5161 tgattcggca accgcgcagt catcgcccgc gctaaccgtt gcgcccactt ccggcaggtc
     5221 aacaaacacc atatcgccta acagctcctg ggcatgttcg gtgataccaa cggtgtaagt
     5281 gccgccggct tctttacgca gccattcgtg ttctttgctg tatttcagtt ctgctggtac
     5341 gttgctcatc aatcaatctc caaaaaagta aatcacgcga cggctttacc gttacgcaca
     5401 aaaacaggtt tcgtcacttt aaccggcatt tcacggttgc gaatttgtac aatcgctgtt
     5461 tcaccaatac cttccggcac gcgcgccagc gcaatgctgt aacccagcgt cggggagaaa
     5521 gtaccgctgg tgataatgcc ttcatgctgg ttgccctgcg catcggtaaa gcgtaccggc
     5581 agttcattac gcagcacgcc tttttcggtc atcaccagac caaccagttt ttctgtgcca
     5641 tgctcacgct gcacttccag ggcttcacga ccgatgaagt cacgatctgc cggttcccag
     5701 gcgatagtcc agcccatatt ggcggctaaa ggagagatgg tttcgtccat ctcctgacta
     5761 taaagattca tacccgcttc cagacgcagc gtgtcacgcg cgcccaggcc gcatggctta
     5821 acgcccgctt ccaccagcgc gcgccagaaa tccgccgctt tttcattggg cagcgcaatt
     5881 tcatagcccg cttcaccggt ataaccagtg gtggcaataa acagatcgcc cgcctgcacg
     5941 ccaaagaacg gtttcatccc ttccaccgcc tgacgctggg cgtcattaaa cagtgtggca
     6001 gcttttgcct gcgcattcgg cccttgtacg gcgatcatgg aaaggtcatc acgaacggta
     6061 atttcgatgc cgaaaggttc agcgtgttgg gtaatccagg agaggtcttt ttcgcgggtg
     6121 gcggagttaa caacgaggcg gaagaaatct tcagtaaagt agtagacgat gaggtcatct
     6181 atcacaccgc cagaggcatt caacatcccc gagtaaaggg ctttgccgct tttggtgagt
     6241 ttcgccacat cgttcgccag cagataacgc agaaactccc gagtgcggct gccgcgaaga
     6301 tcgacgatgg tcatatgtga cacatcaaac attccggcat cggtacgtac cgcatgatgt
     6361 tcgtcgattt gcgaaccgta atgcagcggc atcatccagc cgtggaaatc caccatgcga
     6421 gcgccgcaaa gcgtgtgttg ttcgtacaaa ggagtctgtt gtgccatctt gtcctcattg
     6481 aataagcggg gctgacaact tttccatggc gaaattatca ccacgaaacc cagcatcgga
     6541 gccactcccg gtccccaacg caatcgttct cttttgcctg aacttaccac cgaaacagac
     6601 tgttaaccat aaggtaaaat tgatcatcac attagcttat ggttaaaaaa tgcaaaaatc
     6661 gcaacagaat aaaaaaccaa aaaacacatc agtttctata caaagatgat gtgatgagaa
     6721 agttaatttg aataagacaa tattaagcgc taaaaaaatg tcaaa
//
LOCUS       DAZFLD010000219         6760 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.220, whole genome shotgun sequence.
ACCESSION   DAZFLD010000219 DAZFLD010000000
VERSION     DAZFLD010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6760
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.220"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(83..751)
                     /gene="artM"
                     /locus_tag="ACHKBL_004132"
     CDS             complement(83..751)
                     /gene="artM"
                     /locus_tag="ACHKBL_004132"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HFO5820924.1"
                     /translation="MFEYLPELMKGLHTSLTLTVASLIVALILALIFTIILTLKTPVL
                     VWLVRGYITLFTGTPLLVQIFLIYYGPGQFPTLQEYPALWHLLSEPWLCALIALSLNS
                     AAYTTQLFYGAIRAIPEGQWQSCSALGMSKKDTLAILLPYAFKRSLSSYSNEVVLVFK
                     STSLAYTITLMEVMGYSQLLYGRTYDVMVFGAAGIIYLVVNGLLTLMMRLIERKALAF
                     ERRN"
     gene            complement(751..1467)
                     /gene="artQ"
                     /locus_tag="ACHKBL_004133"
     CDS             complement(751..1467)
                     /gene="artQ"
                     /locus_tag="ACHKBL_004133"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HFO5820925.1"
                     /translation="MNEFFPLASAAGMTVGLAVCALIVGLALAMFFAVWESAKWRPVA
                     WAGSALVTVLRGLPEILVVLFIYFGSSQLLLTLSDGFTINLGFVQIPVQMDIENFDVS
                     PFLCGVIALSLLYAAYASQTLRGALKAVPVGQWESGQALGLSKSAIFFRLVMPQMWRH
                     ALPGLGNQWLVLLKDTALVSLISVNDLMLQTKSIATRTQEPFTWYIVAAAIYLVITLL
                     SQYILKRIDLRATRFERRPS"
     gene            complement(1474..2205)
                     /gene="artI"
                     /locus_tag="ACHKBL_004134"
     CDS             complement(1474..2205)
                     /gene="artI"
                     /locus_tag="ACHKBL_004134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein ArtI"
                     /protein_id="HFO5820926.1"
                     /translation="MKKVLIAALIAGFSLSATAAETIRFATEASYPPFESIDANNQIV
                     GFDVDLAQALCKEIDATCTFSNQAFDSLIPSLKFRRVEAVMAGMDITPEREKQVLFTT
                     PYYDNSALFVGQQGKYTSVDQLKGKKVGVQNGTTHQKFIMDKHPEITTVPYDSYQNAK
                     LDLQNGRIDSVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQ
                     KLNTALEKVKKDGTYETIYNKWFQK"
     gene            complement(2223..2951)
                     /gene="artP"
                     /locus_tag="ACHKBL_004135"
     CDS             complement(2223..2951)
                     /gene="artP"
                     /locus_tag="ACHKBL_004135"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HFO5820927.1"
                     /translation="MSIQLNGINCFYGAHQALFDITLDCPQGETLVLLGPSGAGKSSL
                     LRVLNLLEMPRSGTLNIAGNHFDFTKTPSDKAIRDLRRNVGMVFQQYNLWPHLTVQQN
                     LIEAPCRVLGLSKDQALARAEKLLERLRLKPYSDRYPLHLSGGQQQRVAIARALMMEP
                     QVLLFDEPTAALDPEITAQIVSIIRELAETNITQVIVTHEVEVARKTASRVVYMENGH
                     IVEQGDASCFTEPQTEAFKNYLSH"
     gene            complement(3169..3684)
                     /gene="ybjP"
                     /locus_tag="ACHKBL_004136"
     CDS             complement(3169..3684)
                     /gene="ybjP"
                     /locus_tag="ACHKBL_004136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFO5820928.1"
                     /translation="MRYSKLTMLIPCALLLSACTTVTPAYKDNGPRTGSCVQGGPDSV
                     AQQFYDYRIQHRGNDITALRPYLSDKLATLLSDASRDNSHRELLSSDPFSSRTTLPDS
                     AHVASASTIPNRDARNIPLRVDLKQGDQGWQDEVLMIQEGQCWVIDDVRYLGGSVHAT
                     AGTLRQSIENR"
     gene            3810..4133
                     /gene="ybjQ"
                     /locus_tag="ACHKBL_004137"
     CDS             3810..4133
                     /gene="ybjQ"
                     /locus_tag="ACHKBL_004137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HFO5820929.1"
                     /translation="MQFSTTPTLEGQTIVEYCGVVTGEAILGANIFRDFFAGIRDIVG
                     GRSGAYEKELRKAREIAFEELGDQARALGADAVVGIDIDYETVGQNGSMLMVSVSGTA
                     VKTRR"
     gene            4130..4960
                     /gene="amiD"
                     /locus_tag="ACHKBL_004138"
     CDS             4130..4960
                     /gene="amiD"
                     /locus_tag="ACHKBL_004138"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiD"
                     /protein_id="HFO5820930.1"
                     /translation="MRRVFWLVAVALLLAGCAGEKGIVEKEGYQLDTRRQAQAAYPRI
                     KVLVIHYTADDFDSSLATLTDKQVSSHYLVPAVPPRYNGKPRIWQLVPEQELAWHAGI
                     SAWRGATRLNDTSIGIELENRGWQKSSGVKYFAPFEQAQIQALIPLAKDIIARYHIKP
                     ENVVAHADIAPQRKDDPGPLFPWQQLAQQGIGAWPDAQRVNFYLAGRAPHTLVDTASL
                     LELLARYGYDVKPDMTPREQRRVIMAFQMHFRPTLYNGEADAETQAIAEALLEKYGQD
                     "
     gene            complement(4957..5970)
                     /gene="ybjS"
                     /locus_tag="ACHKBL_004139"
     CDS             complement(4957..5970)
                     /gene="ybjS"
                     /locus_tag="ACHKBL_004139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFO5820931.1"
                     /translation="MKVLVTGATSGLGRNAVEFLCQKGISVRATGRNEAMGKLLEKMG
                     AEFVPADLTELVSSQAKVMLAGIDTLWHCSSFTSPWGTQQAFDLANVRATRRLGEWAV
                     AWGVRNFIHISSPSLYFDYHHHRDIKEDFRPHRFANEFARSKAASEEVINMLSQANPQ
                     TRFTILRPQSLFGPHDKVFIPRLAHMMHHYGSILLPHGGSALVDMTYYENAVHAMWLA
                     SQEACDKLPSGRVYNITNGEHRTLRSIVQKLIDELNIDCRIRSVPYPMLDMIARSMER
                     LGRKSAKEPPLTHYGVSKLNFDFTLDITRAQEELGYQPVITLDEGIEKTAAWLRDHGK
                     LPR"
     gene            complement(6069..>6760)
                     /locus_tag="ACHKBL_004140"
     CDS             complement(6069..>6760)
                     /locus_tag="ACHKBL_004140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HFO5820932.1"
                     /translation="NVITSVPPTTARALIQGLKHDLLADDTALRALIPQRLIAFDDAV
                     RRTLKEEEKLVNSSDWGYDAQAFARWRPEYGYFAKQAGFTVKASASLAALWQVVNQIG
                     GKERYFFGNILWQTRALMDRAIGHKLAKGRPEREYLQTGDAVDSWKVIVVEPEKQLTL
                     LFGMKAPGLGRLCFTLEDKGDYRTIDVRAFWHPHGMPGLFYWLLMIPAHLFIFRGMAK
                     RIARLAEQSTD"
BASE COUNT         1635 a         1781 c         1792 g         1552 t
ORIGIN      
        1 tccgtcttca ttataagaat ttatgcacta tgtaggccgg ataaggcgtc cgcgccgcat
       61 ccggcatcag cacgcagcca aattaattcc ttcgctcaaa cgccagcgct ttacgctcga
      121 tcagacgcat catcagcgtc agcaggccgt taacgaccag ataaataatc cctgccgcgc
      181 cgaacaccat gacatcgtag gtgcgtccgt acaacaactg gctgtatccc atcacttcca
      241 tcagcgtaat ggtgtaggcc agcgaggtac ttttgaacac cagcactact tcgttggaat
      301 aagaagagag cgagcgttta aaggcatacg gcagcaggat cgccagcgta tcttttttgc
      361 tcattcccag ggcgctacag gactgccact gaccttccgg gatcgcacga attgcaccgt
      421 aaaacagctg cgtggtatac gccgcactgt tcagcgacaa cgcaatcagc gcacacagcc
      481 acggttctga caacaaatgc cacagtgccg gatactcctg caacgtcggg aactggcccg
      541 gtccgtaata aatcaggaag atctgcacca gcagcggcgt accggtaaac agcgtgatat
      601 agccccgcac cagccacacc agcaccggcg ttttcagcgt caggatgatg gtaaaaatca
      661 atgccagaat cagtgccaca atcagcgagg caacggttag cgtcaggctg gtgtgcaacc
      721 ctttcatcag ttcgggtaaa tactcaaaca ttagctgggc ctccgctcaa aacgtgtcgc
      781 gcgcaggtca atgcgtttga gaatgtactg actgagcagg gtgatcacca ggtaaatcgc
      841 cgccgccaca atgtaccagg taaacggttc ctgggtacgg gtagcgatgc ttttggtttg
      901 tagcattaaa tcattcacac taatcaaact gaccagcgcg gtatctttca gcagcaccag
      961 ccactggtta ccgagacccg gcagcgcatg acgccacatc tgcggcatca ccagacggaa
     1021 aaagatagcc gatttcgaca gccccagcgc ctgaccagat tcccactggc ccaccggcac
     1081 cgctttcaac gcgccgcgca gcgtttgcga ggcataggcg gcatacagca gtgacagagc
     1141 gatgacacca caaaggaacg ggctaacgtc gaagttctca atgtccatct gcaccgggat
     1201 ctgcacgaac ccaagattga tagtgaagcc atccgaaagc gtcagcagca gctgcgagga
     1261 gccaaaatag ataaacagca ccaccagaat ttctggcagg ccgcgcagaa ccgtcaccaa
     1321 cgctgaacct gcccacgcga caggacgcca ttttgcagac tcccataccg caaagaacat
     1381 cgccagcgcc agcccgacaa tcaatgcaca aacggcaagg ccgacggtca tcccggcggc
     1441 gcttgctaaa ggaaaaaatt cattcatcag gaattacttc tggaaccatt tgttgtagat
     1501 ggtttcgtaa gtgccatctt tcttcacttt ttccagcgca gtgttgagtt tctgctgcag
     1561 ttcagtgttg ccctggcgta ctgcgatgcc gaggccagta ccgaagtaat ctttatcggt
     1621 cactttatcg cccactgccg ccagtttcgg gttatctttc agccattcgg ttaccactgc
     1681 ggtgtcacca aatacgctgt cgatacgacc gttttgcaga tccagttttg cgttctggta
     1741 actgtcatat ggcacggtag tgatttccgg atgcttatcc ataatgaact tctggtgtgt
     1801 cgtcccgttc tgaacgccga cttttttgcc tttcagctga tcaacactgg tgtatttgcc
     1861 ttgctgaccc acaaacaggg cagagttgtc atagtacggg gtggtaaaca gcacctgctt
     1921 ttcacgctcc ggagtgatat ccatgcccgc catcactgct tctacgcgac ggaatttcag
     1981 gcttgggatc aggctgtcaa acgcctggtt agagaaagtg caggttgcat caatctcttt
     2041 acacaatgcc tgcgccaggt cgacatcaaa accaacgatc tggttgtttg catcaatcga
     2101 ttcaaacgga ggataggagg cttcggtggc aaaacgaatg gtttcggctg ctgtggcgga
     2161 aagactaaaa cctgcaatta acgcggcaat cagaactttt ttcattgttg ttatcccgaa
     2221 tcttagtgag agagatagtt tttaaatgct tcggtttgcg gctcggtaaa gcagctcgcg
     2281 tcgccttgtt ctacgatatg accgttttcc atatacacca ctcggctggc ggttttacgc
     2341 gccacttcaa cttcgtgggt gacgatcacc tgggtaatat tcgtttctgc cagctcacga
     2401 atgatgctga cgatttgtgc cgtaatttcg gggtccagtg cggcggtcgg ttcatcgaac
     2461 agcagtacct gcggttccat catcaacgca cgggcaatag caacacgctg ctgctgacct
     2521 ccagaaagat gcagcgggta acgatcgcta taaggtttga gacgcagacg ttccagcagt
     2581 ttttctgcac gggccagcgc ctgatcttta ctcaacccca gtacacgaca gggcgcttca
     2641 atcaggtttt gctgcacggt cagatgcggc cacaggttgt attgctgaaa caccatgcca
     2701 acgttacgac gcaaatcgcg aatcgctttg tcagaggggg ttttggtgaa atcgaaatgg
     2761 ttgcctgcaa tgttgagcgt accggagcgc ggcatctcaa gcagattgag tacacgcagc
     2821 agcgagcttt tacccgcgcc gctggggcca agtaacacca gcgtttcgcc ctgtgggcaa
     2881 tccagcgtga tatcgaacag cgcctgatgc gcgccgtaga agcaattaat gccgtttaat
     2941 tgaatactca ttgacactcg tatactggca gtctgatagc tattgaggtc gaagatagta
     3001 cctttgacag aataattatg caatatttct gctttaaaag ttaaaagcaa agcgcattat
     3061 tcaataaaca tagcacaaaa taacgggggc ggtggtcggc gagcataaat gtcggcattc
     3121 ctcacgaaat gccgtacaat ttacggggtt tattggttga tcaagacgtt agcgattctc
     3181 gatggactga cggagcgtac ccgctgtggc atgaacgcta ccgcctaagt agcgcacatc
     3241 gtcgatgacc caacactggc cttcctgaat cattaacact tcatcctgcc agccctggtc
     3301 accctgcttg agatccacgc gcaatggaat gttacgggca tcacgattag ggatagtcga
     3361 tgcactggca acgtgggcgc tgtctggcaa ggtggttcga ctggagaacg gatcgctgct
     3421 taacaattca cgatgactat tatcgcggct ggcatcgcta agtagggttg ccagtttgtc
     3481 actcaaatag gggcgcagag cggtaatgtc attgccgcga tgttgaatgc ggtagtcata
     3541 gaactgttga gccacgctat caggcccccc ctggacacag gaaccggtgc gggggccgtt
     3601 atctttataa gctggcgtga cggtggtaca ggcactgagt agcagtgcgc aagggataag
     3661 catggtcaat ttgctatagc gcatgatgat ttccttataa gcgaacacct tgaaagcgtt
     3721 ctacgataat aatgatatcc tttcaataat agcgtatcag tccgataatg ctttgaagat
     3781 cgaaggctta gcaaacaagg agatcgatca tgcaattttc tacaacccca actctggaag
     3841 gccagaccat cgtcgaatat tgcggtgtgg tgaccggcga agcgatttta ggtgccaata
     3901 ttttccgtga tttctttgcc ggtatccgcg atatcgttgg cggacgctcc ggggcttatg
     3961 aaaaagaatt gcgtaaagcg cgggagatcg cctttgagga gctcggcgat caggcccggg
     4021 cgctgggggc tgatgccgta gtcggtattg atatcgacta cgaaacggtc gggcaaaatg
     4081 gcagtatgct gatggttagc gtcagcggta cggcggtgaa aacgcgtcga tgagaagagt
     4141 cttttggctg gtcgctgtcg ctctgttact ggcagggtgt gcaggcgaaa aaggcattgt
     4201 cgagaaagag ggatatcagc ttgatacccg acgccaggcg caggcggcgt atccgcgcat
     4261 taaagtgctg gttatccact acaccgcaga tgattttgat agctcgctgg cgacactgac
     4321 cgataagcag gtcagctcgc attatctggt ccctgcggta ccaccgcgat acaacggtaa
     4381 accgcgcatc tggcaactgg tgccggaaca ggaactggcc tggcatgcgg ggattagcgc
     4441 ctggcgcggg gcaacgcgtc ttaacgacac ctctattggc attgagctgg aaaaccgtgg
     4501 ctggcaaaaa tcatccggag tgaaatattt tgccccgttt gaacaggcac agattcaggc
     4561 gcttattccg ctggcgaaag atattattgc ccgttatcac atcaagccgg aaaacgtagt
     4621 ggcgcatgcg gatattgcgc cgcagcgcaa agacgatccg gggccgttat tcccctggca
     4681 acaactggcg cagcagggga ttggtgcctg gccggatgcg cagcgggtga acttttacct
     4741 tgccgggcgc gcgccgcaca cactggtaga taccgcatca ttactggagc ttttggcgcg
     4801 ctacggttat gacgttaaac ctgatatgac accgcgcgag cagcggcgcg tgattatggc
     4861 gttccagatg catttccgcc cgacgttata caacggcgaa gcggatgcag aaactcaggc
     4921 gattgccgaa gcgttgctgg agaaatacgg gcaggattag cgcggcagtt ttccgtggtc
     4981 gcgtagccag gcggcagttt tctcgatacc ttcatccagg gtgatgaccg gctgataacc
     5041 taactcttcc tgcgcgcgcg taatatccag cgtaaagtca aaattcaact tggagacgcc
     5101 gtagtgggtc agcggcggct cttttgctga cttgcggcct aaacgctcca tgctgcgggc
     5161 gatcatatcc agcatcgggt aggggacgga acgaatacga cagtcaatat tcaactcgtc
     5221 gatcagcttc tgcacgatgc tgcgcagtgt gcgatgctcg ccgttggtga tgttgtacac
     5281 acgcccggaa ggtagcttat cgcaggcttc ctggcttgcc agccacatgg cgtgcacggc
     5341 attttcatag taggtcatat cgaccagcgc actgccgcca tgcggtaaca gaatgctgcc
     5401 gtagtggtgc atcatatgcg ccagacgggg aataaagact ttatcgtgcg gtccgaacag
     5461 actttgtggg cgcagaatag taaagcgcgt ttgtggattc gcctgcgaaa gcatattgat
     5521 cacctcttcg ctggctgctt tgctacgggc aaactcgttg gcgaagcggt gagggcgaaa
     5581 atcttcttta atatcgcgat ggtggtgata atcgaagtac agggagggtg aagagatatg
     5641 aataaagtta cgcacacccc aggcgacagc ccattcaccc aggcggcgag tggcgcgaac
     5701 gttagccaga tcgaaagcct gttgtgtccc ccagggcgag gtaaagctgg agcagtgcca
     5761 cagcgtatca atgcccgcga gcatcacttt agcttgtgat gagaccagct cggtcagatc
     5821 cgccggaaca aactctgcgc ccattttttc cagcaattta cccattgcct cgttgcgacc
     5881 ggtcgctcgc acgctgatgc ctttctggca taaaaactct accgcgtttc gacctaagcc
     5941 gctggtggcg ccggtaacca gtaccttcat atcaatccac tgttgttgag aaaataacgt
     6001 gcgcattctt ccgtgatttc ccccatgatg caatgggaaa catgaaagaa taacgcaggt
     6061 tttgtcgatt aatctgtgct ttgttctgcc agtctggcga ttcgttttgc cattccgcga
     6121 aaaataaaca gatgcgcggg gatcatcaat aaccagtaaa acagccccgg cataccgtgc
     6181 ggatgccaga atgcgcgaac atcgatagta cgatagtcac ctttatcttc cagggtaaaa
     6241 cacagtcgtc ccagccccgg cgctttcatg ccaaataaca gcgtaagttg tttttccggt
     6301 tcaacgacaa tcactttcca gctatccacc gcatcgccgg tctgcaaata ttcacgctcc
     6361 gggcggcctt tcgccagctt atgaccgatc gcgcggtcca tcaacgcccg tgtctgccac
     6421 aaaatattgc caaagaaata acgctcttta ccgccgattt gattcaccac ctgccataaa
     6481 gccgcaaggc tggcggacgc tttaacggta aaccccgcct gtttggcgaa ataaccgtac
     6541 tccggtcgcc agcgggcaaa ggcctgagcg tcgtagcccc agtcgctgga gttgaccagc
     6601 ttttcttctt ctttcagggt gcgacgtacc gcgtcatcga aagcgatcag ccgttgtggg
     6661 atgagtgcac gtagcgcggt gtcatccgcc agcagatcat gtttcagccc ctgaatcaac
     6721 gccctggcgg tggtgggcgg tacggaagta atcacattga
//
LOCUS       DAZFLD010000220         6722 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.221, whole genome shotgun sequence.
ACCESSION   DAZFLD010000220 DAZFLD010000000
VERSION     DAZFLD010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6722)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6722)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6722
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.221"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(123..335)
                     /locus_tag="ACHKBL_004141"
     CDS             complement(123..335)
                     /locus_tag="ACHKBL_004141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000917251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibitor protein"
                     /protein_id="HFO5820933.1"
                     /translation="MLKPHYGTALVSREDVKPGTAILYNGRYYMASANVNNALYAHSL
                     IEKIRIISDAIEVYLNNKGQPLISPA"
     gene            complement(406..1035)
                     /locus_tag="ACHKBL_004142"
     CDS             complement(406..1035)
                     /locus_tag="ACHKBL_004142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000211520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antA/AntB antirepressor family protein"
                     /protein_id="HFO5820934.1"
                     /translation="MTTQLIPVFEGTISNEPTLLVNARDLHGFLEVGKDFSNWIRARL
                     NEYGFVENLDYILFSPNLAKTPGRRRKDYHLTLDTAKELAMVERNEKGRQIRRYFIEC
                     EKKLRQSLLPAPMNINYPLSWFSEHHPYATMNYVDRKVISLDASVLFDMPSPTMRILN
                     ELHSKGYNVDAAVAEFNAFKHLTEEMRRKLLDISRTSEKSSRFGFNVNL"
     gene            complement(1304..1570)
                     /locus_tag="ACHKBL_004143"
                     /pseudo
     CDS             complement(1304..1570)
                     /locus_tag="ACHKBL_004143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309278.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="antA/AntB antirepressor family protein"
     gene            complement(1836..2387)
                     /locus_tag="ACHKBL_004144"
     CDS             complement(1836..2387)
                     /locus_tag="ACHKBL_004144"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5820935.1"
                     /translation="MMRKILRTTLITTIFFSPIVQAEWYTVTDDDLFSDGHTALMVGK
                     KTSNPEEIDNHMIALNCTPRSLTFSLVEKDIEEETSAPIYSFPIDVAIKIDKNSIIRL
                     NATLSRRNSMLTQAITERTDTEMKKIKIILRQLRDAKHRVRVGIRRQDITREDAMTYS
                     FNFNVLGSTGAVNKFITACGIKL"
     gene            complement(3070..4023)
                     /locus_tag="ACHKBL_004145"
     CDS             complement(3070..4023)
                     /locus_tag="ACHKBL_004145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001064713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BsuBI/PstI family type II restriction
                     endonuclease"
                     /protein_id="HFO5820936.1"
                     /translation="MNNQNDYIEAAQQIIASLGLPRAQQNERSALCLLALLNLTPGKA
                     WADAENPLVGITPIMNWVREHYGKVYAPNTRETFRRQSMHQFCAAGVALYNPDKPDRP
                     VNSPKAVYQIEPAALSMLRTFGSPAWHDSLATYLAERETLVTRYAKEREQNRIPVEIA
                     AGQQITLSPGEHSELIRAIIEDFAPRFAPGSVLVYAGDTGEKWGYFDAPLLAGLGVDV
                     DSHGKMPDVVLHFTAKNWLLLVESVTSHGPVDGKRHAELARLFAGSTAGLVYVTAFPN
                     RSIMGRYLGEIAWETEVWVADAPSHLIHFNGVRFLGPYSTE"
     gene            complement(4020..5489)
                     /locus_tag="ACHKBL_004146"
     CDS             complement(4020..5489)
                     /locus_tag="ACHKBL_004146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Eco57I restriction-modification methylase
                     domain-containing protein"
                     /protein_id="HFO5820937.1"
                     /translation="MLQKLDVADSVRRELSPRTARKHKAEFGQFMTPSGVARFMASLF
                     PPSTMKTCRLLDAGAGVGALSCAFLDRWLVGEFGFESVEATAYEIDKKLCGHLAKHLT
                     GYRRVTPRIIEGDYIELATAEGLQDRGYTHAILNPPYKKINSQSAHRQALRTVGIETV
                     NLYSAFVALAVGEVAPGGQIVAIIPRSFCNGPYYRPFRNFILERAAIRHIHLFESRSK
                     AFRDDDVLQENIIIRLERGGRQEAVTVTTSTDDSFFDLVTYEHPFDQIVYPDDSERFI
                     HVPTTLEKSTIELSSAVQCSLADIGVKVSTGPIVDFRLKAHLRSMPEEGTVPLIYPSH
                     LSMSSTVWPVEGLKKPNAIMRNDETEKWLYPNGFYCVVRRFSSKEEKRRVVASVVDPA
                     TFSEYSVLGFENHMNVFHENKHGLPEALARGLAVFLNTTAVDKYFRRFNGHTQVNATD
                     LKMIKYPSRDTLIELGKWAMQQETLTQEHIDAKLGALTA"
     gene            complement(5584..6297)
                     /locus_tag="ACHKBL_004147"
     CDS             complement(5584..6297)
                     /locus_tag="ACHKBL_004147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family protein"
                     /protein_id="HFO5820938.1"
                     /translation="MNMKKKPLTPEQLEDAKRLKSIFNAKKKELGLSQESLAYELGVT
                     QSAVNQLMAGINAINASHAAQLAKILNVKVGDFSPSLAKSIAEMALAIEEPLTRVPAY
                     EYPLLSCVQAGAFSMDDISYTAKDAIKWISTTTKASDRSFWLEVKGHSMTAPQGGKPS
                     FPEGMLILVDPEREIEDGDFCVARMNGDEFTFKRFIRESGKAYLEPLNPRFDMIECNE
                     NCQFVGKVIKSQWNDETFD"
     gene            6393..6596
                     /locus_tag="ACHKBL_004148"
     CDS             6393..6596
                     /locus_tag="ACHKBL_004148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001240876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/CI family transcriptional regulator"
                     /protein_id="HFO5820939.1"
                     /translation="MAMKKETLADYVYRFGQKKAAKDFGVAQSAISKALLVGREIYVK
                     TFDDGTVEAEEVRPFPAFVRGDD"
BASE COUNT         1647 a         1556 c         1665 g         1854 t
ORIGIN      
        1 caggacgaca aaataggcga tgattttatc atccataaaa acttctccca ttatcgtttt
       61 cgctggcgtt atgacgcttt ttacattgat attaatttct tgattgagca tgatatttcc
      121 tttcaggctg gtgagattaa cggttggcct ttattgttca ggtaaacttc tattgcatct
      181 gagataatgc gaattttttc aatcagtgaa tgtgcgtaaa gtgcattatt aacgttcgct
      241 gacgccatgt aataacgccc gttgtaaaga attgctgtgc cgggtttaac gtcctcgcga
      301 gaaactaatg cggttccgta gtgaggtttg agcatgacaa atcctccggt taattaatcc
      361 agatatttta tttaatcccc gatatgtgat cggggatggg gttaattaaa gatttacgtt
      421 gaaaccaaag cgggaagact tttccgatgt gcgggaaata tccagcaatt tacggcgcat
      481 ttcttccgtc agatgcttga aggcgttaaa ttcggcgaca gcggcgtcaa cgttatagcc
      541 tttgctgtgt agctcattga ggatgcgcat agttgggctt ggcatatcga agagcacgga
      601 agcatcaagg ctgataacct tgcgatcaac atagttcatc gtggcatagg ggtgatgctc
      661 tgagaaccac gagagggggt agttgatgtt cattggtgca ggtaaaaggc tctggcgaag
      721 tttcttttcg cactcgatga agtatcggcg gatctgacgg cctttttcgt tacgttcaac
      781 catcgcaagc tctttggctg tgtcgagggt gaggtggtaa tccttgcggc ggcggcctgg
      841 agttttcgcc aaatttggcg aaaatagaat gtagtctaaa ttttctacga atccatactc
      901 gtttagcctg gcccgtatcc agttagaaaa atctttacct acctctaaaa atccatgcaa
      961 atcacgcgcg ttcaccagaa gcgtaggttc atttgagatt gtaccttcaa acacagggat
     1021 gagttgagtg gtcatgatga cctcctttgg cttttttcga gaaatgccac cacaagatgt
     1081 ggtgccggga ggctcgaaac ggcccaaaag ataccgcgga cttattcccc ttgcgggtgt
     1141 tgtattcgtc gccctcccga cattgatcgg gggatgtgac cgcacactgt gctatcactg
     1201 aataacaggc ataaaaaatc caacactgac ggggttggtt taatccgctt ttgggaggtt
     1261 ttcgaggcct cggcgcggag tatagtcagt attggacgca gtcgtcaacc atcgcaagct
     1321 ctttggctgt gtcgagggtg aggtggtatt ccttgcggtt gtgaccgcct ctaccttttg
     1381 cttcccgaat ttgggaagca atcatatagt cctgattttc aacgaaacca tattcagcaa
     1441 tgcgttctgt aatccaagac gcaaagcgtt tacctacccc aagaaaagta tgtaaatcac
     1501 gggcattaca gaacagggta gtttcgttgg cgatggtgcc gttgaatacg gggatgagtt
     1561 gagagttcat attttatgtc ctttgcattt gttcagatag ccctgtgttc agcagggcgg
     1621 ccaggtactt gaacaccgca gcaagtcggc ccgtatcctt agccttacgg ctgtttttcg
     1681 gtatacgcgc tacccgacca tatctgaaaa atggacacaa aaaaaccgca tgactgacgg
     1741 ggcggtttcc gcttgctgtg gtgtgttcag caccatgaag cggaatatat ccccgtcgat
     1801 gtggatttgt caaggttgct ttggcagaaa catgtttaca gctttattcc gcatgcagtg
     1861 ataaatttat taacagcacc agtggagcct aatacattaa agttgaaaga atatgtcatg
     1921 gcatcttctc gtgtgatgtc ttggcgtcga atcccgaccc tcactctatg tttagcatct
     1981 ctgagttgcc tgagtataat tttaattttc ttcatttcag tatcagtacg ttcagtgata
     2041 gcttgtgtta gcattgagtt tcttcttgaa agtgttgcgt tgagccttat tatactattt
     2101 ttgtcaattt ttattgccac atctattgga aatgaataaa ttggggccga tgtttcttct
     2161 tctatgtcct tttcgactaa tgagaatgtt agggatcttg gcgtacaatt aagagcaatc
     2221 atgtgattat caatctcttc aggatttgat gttttttttc ctaccatcag cgccgtgtga
     2281 ccatcagaga acaaatcgtc gtctgttact gtataccact ccgcctgtac tattggtgaa
     2341 aaaaaaattg tagtgataag agttgtcctg agtatttttc gcatcattaa gtttcccgtt
     2401 cagttcgact gaccacttca aagtgcttac ataggctatt tgatagcact acctacgcgg
     2461 cagtataaag aagtcagttg aaaacttgaa cgttgtgttg aaaacataac tttgtgcggc
     2521 ataggtaatc gtagaatgat tgtgattggt cttatggtat ccaatcatta cttttgctat
     2581 aggttttgga cggaatcggt atttcatgta acgctttggc ggtgctgcct gcaccccaaa
     2641 acattccctg tattggtcag cgccaacttc ctgccagtgt tgcccgttct cacgccgttc
     2701 tcgctctcgc gcggggatac tctctcatcg accggatcgc acccgatgat acagcacgtt
     2761 tacgtgtagg ggtctaaaca ggtcattgac gctgtaaagc tccagattgt taaagagcat
     2821 tttgcggcgg gttaagtcgc gccgtacgac tgatttatgt agccctgtgt aagggcgcga
     2881 tgtttctggc ttgaaataaa tataacttgc ggtgattttc atgtaaatac cggtggtgct
     2941 ttttaagggc gagaaattat tacttaactg atttttaaag tgatttattt tttaaggggg
     3001 cagataacag gaggggatat gcaaagaaaa acccggcgca tgggccgggc tatttatctg
     3061 gtcctgttct cattcagtag agtaggggcc aagaaatcgc actccgttga agtgaataag
     3121 atgagaaggt gcatcagcca cccatacctc tgtttcccat gcgatttcac caagatatcg
     3181 ccccatgatg gagcgatttg gaaaagcggt cacatagaca agtccggctg ttgatccggc
     3241 aaacagcctg gcaagctcag catgccgctt cccatcaacc ggtccatgac tggtgacaga
     3301 ctcaaccagt agcagccagt ttttcgcagt aaaatgtagc accacatctg gcattttacc
     3361 gtgtgaatcc acatcaacac ctagcccggc cagcagtgga gcgtcgaagt agccccactt
     3421 ttcgccagtg tcgccagcat agaccagcac gctacccgga gcaaaacgtg gggcgaagtc
     3481 ctctattatg gcacggataa gttcgctgtg ctcgccggga ctgagggtta tttgctggcc
     3541 cgcagcaatt tcaacaggga tacgattctg ttcgcgctcc ttagcatagc gggtaaccag
     3601 cgtttcacgc tcggctaaat aggttgcaag gctatcgtgc catgccgggc tgccgaaagt
     3661 gcgtagcatg gacagagcag caggttcgat ctgataaaca gccttcgggc tgttcactgg
     3721 gcgatcgggc ttgtccggat tgtagagggc tactccagca gcgcagaact gatgcataga
     3781 ctggcggcgg aatgtctcac gagtattggg tgcgtagacc ttgccgtagt gctcccgaac
     3841 ccaattcatg attggtgtaa tgcccacaag cggattttcc gcgtcggccc atgcttttcc
     3901 cggtgtgagg ttcaggagtg ccagcaaaca caaagcagaa cgctcattct gctgcgctct
     3961 gggcaggcct aaggatgcta taatttgctg agcggcctca atataatcat tttggttgtt
     4021 catgcagtca gagctcctag cttagcgtca atatgttctt gcgtgagagt ttcttgttgc
     4081 atagcccact tgccaagctc tatcagagtg tcacggcttg ggtacttaat catcttgagg
     4141 tcggttgcat tgacctgtgt atgcccgttg aagcgacgga aatacttatc cactgcggta
     4201 gtgttcagga acacggccaa gccacgggcc agcgcctcgg gtaacccatg tttgttctca
     4261 tgaaacacgt tcatgtgatt ttcaaatccc aacactgaat actcactgaa ggtagcagga
     4321 tcaacgacgc ttgctaccac tcggcgcttt tcctccttcg acgagaaacg acgcaccaca
     4381 caataaaaac cgttcgggta aagccacttt tctgtctcgt cgttgcgcat gattgcgttg
     4441 ggtttcttca aaccttccac aggccagaca gtgctactca tactcaaatg gctcgggtag
     4501 atcaggggaa cagttccttc ttctggcatg ctgcgcaaat gagctttcag tcggaaatca
     4561 actatcggtc cggtcgatac cttcacgcca atatcagcca gcgaacattg tacggcagat
     4621 gataactcaa tggtgctttt ctcaagcgtg gtcggcacat gaataaaccg ctccgaatcg
     4681 tccgggtaca caatctggtc aaatgggtgt tcgtaggtga ctaggtcaaa aaagctatca
     4741 tcggtcgaag ttgtgactgt aacagcctcc tgacggcctc cacgctccag gcggatgata
     4801 atattttcct gcaacacatc gtcatccctg aaagctttgc tacgtgattc gaacaaatga
     4861 atatggcgga ttgctgcccg ctcaagtatg aaattacgga acggacggta atacggtccg
     4921 ttgcagaaac tacgtgggat tatggccact atctgcccgc caggtgcaac ctcacctaca
     4981 gccagtgcaa caaaggcgga atacagattt accgtctcga taccgacagt gcgaagagcc
     5041 tgccggtggg ctgactggct attgatcttc ttgtaaggcg gattaagtat tgcatgggta
     5101 taacctcgat cctgtagacc ttcggcggtt gccagctcaa tataatcccc ttcgatgatg
     5161 cggggcgtca ctctcctgta cccggttaag tgcttcgcaa ggtgaccaca cagctttttg
     5221 tcgatttcgt aggctgttgc ctcgacagac tcaaagccaa actcgccaac cagccaacgg
     5281 tcgaggaagg cgcaagacag tgcgcctaca ccagcgcctg cgtccagtag acggcaagtt
     5341 ttcattgtgc tcggtggaaa caaggaggcc ataaaacggg ccacgccaga aggcgtcatg
     5401 aactggccga actctgcttt gtgtttacgc gcggtacggg gagacagctc gcgacgcacg
     5461 ctgtctgcga cgtctagctt ttgaagcata actaatcctc tttacgtggc atgatgatgc
     5521 caacgtgctt ttgacatcct ccacattctt caggcgtgaa ggatgtcaaa tcactctgtg
     5581 agctcaatca aaagtctcat cattccactg cgatttgatg acctttccga caaactggca
     5641 gttttcgtta cactcaatca tgtcgaatcg tgggttgagc ggctccaggt acgctttacc
     5701 gctctcacga atgaatcgtt tgaaggtaaa ttcatcgccg ttcattcggg ccacgcaaaa
     5761 atcgccatcc tcgatttccc gttcagggtc aacgagtatc agcatacctt cagggaagct
     5821 gggtttacca ccctgcggtg cggtcattga atgccccttg acctccagcc agaacgacct
     5881 gtcactggct tttgtggtgg tggagatcca tttaatcgca tcttttgcgg tgtacgaaat
     5941 atcatccatt gagaatgcgc cagcttgtac gcaggagagt aacggatatt cgtaagcggg
     6001 gacacgagtt aatggctctt caattgcgag cgccatttct gcaatagatt ttgccaggct
     6061 gggactgaaa tctccgacct ttacattcag tatcttagca agctgtgctg cgtggctggc
     6121 atttatggcg ttgatgccag ccattagctg attaacagcg ctttgggtaa cacctaattc
     6181 ataggctaat gattcctgcg acaggcctaa ctctttcttt ttagcgttaa aaatactttt
     6241 cagacgcttg gcgtcttcca gttgctcagg ggttaacggt ttctttttca tgttcatacc
     6301 atatcacctt tggttatatg taatgaaata tctgcggtgt tgacatttta ataactgtgc
     6361 gtaatgatat gggcattttg aaggagtcag ttatggcgat gaaaaaagag accctggctg
     6421 attacgtata caggttcggc caaaagaaag ccgctaagga ctttggcgtg gcgcagagtg
     6481 caatcagtaa agcgcttctc gtcggacgag aaatatacgt caaaaccttt gatgacggca
     6541 cagttgaggc ggaagaggtt cgtccgttcc ctgcgttcgt aagaggcgat gattaatcct
     6601 ggacacacag cccatcggag gtgtgagtga acaggcaaat caaaacggtt atgcccgagc
     6661 agtactgtga tgctgatcgg gactggatac aggagcaatt gttacagcta gacccgacca
     6721 cc
//
LOCUS       DAZFLD010000221         6619 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.222, whole genome shotgun sequence.
ACCESSION   DAZFLD010000221 DAZFLD010000000
VERSION     DAZFLD010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6619
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.222"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            32..1693
                     /gene="recN"
                     /locus_tag="ACHKBL_004149"
     CDS             32..1693
                     /gene="recN"
                     /locus_tag="ACHKBL_004149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HFO5820940.1"
                     /translation="MLAQLTISNFAIVRELEIDFHSGMTVITGETGAGKSIAIDALGL
                     CLGGRAEADMVRTGAARADLCARFSLKDTPAALRWLEENQLEDGHECLLRRVISSDGR
                     SRGFINGTAVPLSQLRELGQLLIQIHGQHAHQLLTKPEHQKFLLDGYANETSLLQEMT
                     ARYQLWHQSCRDLAHHQQLSQERAARAELLQYQLKELNEFNPQPGEFEQIDEEYKHLA
                     NSGQLLTTSQNALAVMADGEDANLQSQLYTAKQLVSELIGMDSKLSGVLDMLEEATIQ
                     IAEASDELRHYCDRLDLDPNRLFELEQRISKQISLARKHHVSPEALPQYYQSLLEEQQ
                     QLDDQADSQETLALAVTKHHQQALETARALHQQRQQYAEELALLITDSMHALSMPHGQ
                     FTIDVKFDEHHLGADGADRIEFRVTTNPGQPMQPIAKVASGGELSRIALAIQVITARK
                     METPALIFDEVDVGISGPTAAVVGKLLRQLGESTQVMCVTHLPQVAGCGHQHYFVSKE
                     TDGAMTETHMQSLDKKARLQELARLLGGSEVTRNTLANAKELLAA"
     gene            1842..2183
                     /gene="bamE"
                     /locus_tag="ACHKBL_004150"
     CDS             1842..2183
                     /gene="bamE"
                     /locus_tag="ACHKBL_004150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HFO5820941.1"
                     /translation="MRCKTLTAAAAVLLMLTAGCSTLERVVYRPDINQGNYLTANDVS
                     KIRVGMTQQQVAYALGTPLMSDPFGTNTWFYVFRQQPGHEGVTQQTLTLTFNSSGVLT
                     NIDNKPALSGN"
     gene            complement(2245..2535)
                     /gene="yfjF"
                     /locus_tag="ACHKBL_004151"
     CDS             complement(2245..2535)
                     /gene="yfjF"
                     /locus_tag="ACHKBL_004151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417108.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HFO5820942.1"
                     /translation="MPGKIAVEVAYALPEKQYLQRVTLQEGATVEEAIRASGLLELRT
                     DIDLTKNKVGIYSRPAKLSDSVHDGDRVEIYRPLIADPKELRRQRAEKSANK"
     gene            complement(2525..2962)
                     /gene="ratA"
                     /locus_tag="ACHKBL_004152"
     CDS             complement(2525..2962)
                     /gene="ratA"
                     /locus_tag="ACHKBL_004152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010376629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin RatA"
                     /protein_id="HFO5820943.1"
                     /translation="MPQISRTALVPYSAEQMYQLVNDVQSYPQFLPGCTGSRILESTP
                     GQMTAAVDVSKAGISKTFTTRNQLTSNQSILMNLVDGPFKKLIGGWKFTPLSQEACRI
                     EFHLDFEFTNKLIELAFGRVFKELAANMVQAFTVRAKEVYSAR"
     gene            3133..3615
                     /gene="smpB"
                     /locus_tag="ACHKBL_004153"
     CDS             3133..3615
                     /gene="smpB"
                     /locus_tag="ACHKBL_004153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HFO5820944.1"
                     /translation="MTKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSL
                     RAGKANISDSYVLLRDGEAFLFGANITPMAVASTHVVCDPTRTRKLLLNQRELDSLYG
                     RVNREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR
                     "
     gene            3830..4192
                     /gene="ssrA"
                     /locus_tag="ACHKBL_004154"
     tmRNA           3830..4192
                     /gene="ssrA"
                     /locus_tag="ACHKBL_004154"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            4455..4704
                     /locus_tag="ACHKBL_004155"
                     /pseudo
     CDS             4455..4704
                     /locus_tag="ACHKBL_004155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238123.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
     gene            complement(4917..5390)
                     /locus_tag="ACHKBL_004156"
     CDS             complement(4917..5390)
                     /locus_tag="ACHKBL_004156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5820945.1"
                     /translation="MKVILATRNRYLEYGLQQMLEGYRIILAREFFTPENRKSVPAHD
                     ESWVIICDALLGRLMCCMFQGRRYLQIDAEDVTGRLETYRKIRNGEWVHNTYARPLTM
                     SEMVVMFGYVYRESKPCHLAREMGINTKTVNTFLYMGLGKNGLKYRSVKHLVGRA"
     gene            complement(5477..6601)
                     /locus_tag="ACHKBL_004157"
     CDS             complement(5477..6601)
                     /locus_tag="ACHKBL_004157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HFO5820946.1"
                     /translation="MSVVIGGILFGVSAGVLAQDAAENKLIASGSIPEHTDLSASARA
                     DGEIALAVGTGASADKKFATSVGSWSAARGQQSVALGGATYAYADASTALGSVAFVDS
                     TATYGTAAGNRAKVDKDATEGTALGAKATVTNKNSVALGANSRTTRDNEVYIGYEAES
                     GKAYKTRVLGGLSDGTRPSDAATVRQVDRVKDNVEQLAQDTNTRLVVEAKKFREYTDS
                     RTTVGVNSDGTLTRAEGASKTLSVNDGLVALSGRTDRIDATVGSVDRRVTKNTQAIQS
                     TSRQLQEHSTRLNSHQRQINENHEEMKRAAAQSAALAGLFQPYSVGKFNATAAVGGYS
                     DKQAVAVGVGYRFNEQTAAKAGVAASDGDVSWNAGVNFEF"
BASE COUNT         1767 a         1754 c         1514 g         1584 t
ORIGIN      
        1 cagtaatggt ttttcataca ggaaaacaac tatgttggca caactgacca tcagcaactt
       61 tgctatcgtt cgtgagcttg agattgattt tcatagcggc atgaccgtaa taactggcga
      121 gaccggcgcg ggtaaatcta ttgcaataga tgccctcggc ctttgtctcg gtggtcgcgc
      181 tgaagccgac atggtgcgta ccggcgctgc tcgtgccgac ctgtgcgccc gtttttctct
      241 gaaagatacg ccagcggccc tgcgctggct ggaagaaaac cagcttgaag acggtcatga
      301 atgtttgctt cgtcgcgtaa tcagtagcga tggtcgctca cgaggtttta tcaacggtac
      361 agctgttcct ctgtcacaac ttcgcgaact gggccagttg ctgattcaga tccatggtca
      421 gcacgcccat cagttactca ccaaacccga gcaccaaaaa ttcctgcttg atggctatgc
      481 caatgaaacc tctctgctcc aggaaatgac cgcacgttat cagttgtggc atcaaagctg
      541 ccgtgacctc gcgcatcatc aacagttaag tcaggaacgc gccgcccgtg cggaactgct
      601 gcaataccaa ttaaaagaac ttaacgaatt taatccgcag cccggagagt ttgagcaaat
      661 cgacgaagag tacaaacatc tggcgaacag cggtcaattg ctgactacca gccagaatgc
      721 attggcagta atggccgacg gtgaagacgc aaacctgcaa agtcagcttt acacggctaa
      781 acaactggtg agcgaattga ttggcatgga cagcaaactg tccggcgtac ttgatatgct
      841 ggaagaagct accatccaga ttgctgaagc cagcgatgaa ctgcgccact actgcgatcg
      901 tctggatctc gatcccaacc gactgtttga acttgaacag cgcatctcaa aacagatttc
      961 gctggcacgt aaacatcacg tcagccctga ggcattgcca cagtattacc agtcgctact
     1021 ggaagaacag cagcaactgg acgatcaggc cgactcacaa gaaacgctcg cgctggcggt
     1081 aacgaaacat catcagcagg cactggaaac cgcgcgcgca ttacaccagc aacgccagca
     1141 gtatgcagaa gaacttgcac tgctgatcac cgacagtatg catgcgctct caatgccgca
     1201 tgggcagttt acgatcgatg ttaaatttga cgagcatcac ctgggcgctg acggtgccga
     1261 tcgtattgag tttcgggtaa ccaccaaccc aggtcagcca atgcagccta ttgccaaagt
     1321 cgcatccggt ggtgaattgt cacgcatcgc attggcaatt caggtcatca cggcgcgtaa
     1381 aatggaaacc ccggcactga tttttgatga agtggatgta gggattagcg gtccaacagc
     1441 ggcagttgtc ggcaaactgc tgcgtcaact tggcgaatca actcaggtga tgtgtgttac
     1501 ccacctgcca caagtcgcgg gatgtggtca tcaacactat tttgtcagca aagaaaccga
     1561 tggtgcgatg acagaaacac acatgcaatc cctggataaa aaagcgcggt tacaagagct
     1621 ggcgcgcctg cttgggggca gtgaagtcac acgtaacaca ctggcgaatg cgaaagaact
     1681 gcttgcggcg taaacttttt tcctgcttca cggtcagagt aaacagcaaa acgccgtaag
     1741 accggaaagc aaaaggtttt aaagtgatga aaggtctatt atcatcggca tattacagat
     1801 gagccacgta ctgctcgggc ccgaaaagga atcaaatcac tatgcgctgt aaaacgctga
     1861 ctgctgcagc agcagtacta ttgatgttga ccgcaggctg ttccactctg gagcgagtgg
     1921 tttaccgtcc tgacatcaac caggggaatt atctgaccgc taacgacgta tccaaaatac
     1981 gcgttggcat gacgcaacaa caagttgcgt acgcattggg tacaccgctg atgtccgatc
     2041 catttggtac gaatacctgg ttctatgtct tccgccagca accaggtcat gaaggtgtaa
     2101 cgcagcaaac gctgacgctg acctttaata gtagcggggt gttgaccaat attgataata
     2161 aacctgcgct gagtggtaac taatcataaa gttgctctca aagacgttaa aaaaggtgct
     2221 caatgagcac cttttttctg tctgttattt attcgctgat ttttctgctc gttgcctgcg
     2281 cagctctttc ggatcggcaa tgagagggcg ataaatctcc acccgatcgc catcatgcac
     2341 actatcgctc agttttgccg gacggctata aatgccgact ttgtttttag ttaaatcgat
     2401 atcggtacgc aattccagca agccactggc gcgaatagct tcttcaaccg tcgcaccctc
     2461 ctgcagcgtc acacgctgca ggtactgctt ctcaggtagc gcataagcca cctcaacggc
     2521 aattttacct ggcactgtaa acctctttcg cacgaaccgt aaaagcctgg accatattag
     2581 ccgccagttc tttaaacacg cggccaaagg cgagttcaat caacttattg gtaaactcaa
     2641 aatcgagatg aaactcgata cggcacgcct cctgactcag cggcgtaaac ttccatccac
     2701 cgatcaattt cttgaacggc ccatccacca gattcataag aatactttgg ttactggtca
     2761 actggttgcg ggtagtaaac gttttgctga tcccagcctt agagacatct accgccgcag
     2821 tcatctgccc aggagtggac tccagaatcc gacttccggt acaacccggc aaaaactgag
     2881 gataagactg aacgtcattc actaactgat acatttgctc cgcgctgtag ggtaccagtg
     2941 cggtccggct aatctgaggc ataacaattt ccatcaacaa aaatccaaca aataatatca
     3001 tttatcctgc tgtaaaaaaa acgctatccc ggcgctgggt aacatcgggt tcatgctaag
     3061 atagagcctt gtcccccgca ggattgatat ggggtgtttt cgatttcaga ttaccgatga
     3121 ttcacgacgc ttatgacgaa gaaaaaagca cataaacctg gttcagcgac catcgcgctt
     3181 aacaagcgcg cccggcacga atactttatc gaagaagagt tcgaggcggg acttgccctg
     3241 caaggctggg aggttaaatc cctgcgtgca ggaaaagcca atatcagcga cagctacgtc
     3301 cttctgcgtg acggagaggc atttctgttt ggcgctaaca tcacgccaat ggctgtggcc
     3361 tccacgcatg tggtgtgcga tcctacccgt acccgcaagt tacttctcaa ccagcgcgaa
     3421 ctggactcat tgtacggtcg cgtcaatcga gaaggctata ccgtagtggc gctctccctg
     3481 tactggaaaa atgcctggtg caaagtgaaa atcggcgtcg ccaaaggtaa gaaacagcat
     3541 gataaacgtt cagatatcaa agaacgcgaa tggcaggtgg ataaagcgcg tatcatgaaa
     3601 aacgcccacc gttaaacctg cactgcaatt attgatccgc tcctcaccgc gcctccctct
     3661 ccggcggcgc gaatgcacat cttattggct atcacatccg acccaaatgt tgccatccca
     3721 ttgcttaatc gaataaaaat caggctacat gggtgctaaa tctttaacga taacgccatt
     3781 gaggctggtc atggcactca tgaatctggt atacttaccg ttacacattg gggctgattc
     3841 tggattcgac gggatttgcg aaacccaagg tgcatgccga ggggcggttg gcctcgtaaa
     3901 aagccgcaaa aaatagtcgc aaacgacgaa aactacgctt tagcagctta ataacctgct
     3961 tagagccctc tctccctagc ctccgctctt aggacgggga tcaagagagg tcaaacccaa
     4021 aagagatcgc gtggaagccc tgcctggggt tgaagcgtta aaacttaatc aggctagttt
     4081 gttagtggcg tgtccgtccg cagctggcaa gcgaatgtaa agactgacta agcatgtagt
     4141 accgaggacg taggaatttc ggacgcgggt tcaactcccg ccagctccac caaatataac
     4201 aggattacag ctagacttta acagtaaaaa caagaaatta cagacattga atagactgta
     4261 ggtagacctg aaaaagacaa aaatatacac gcaatatgca cgtgcatttt aaaaaacccc
     4321 agatttctcc gtctgggttt tttctatttg taaaaaagtg aaacagatta cattacaata
     4381 atgacgctct tgacactgtt tatgcataca gtaaaaaatt attcctgtac ataaccacag
     4441 tgacaggagg tgttatgcgt attgagatct gtatcgctaa agaaaaaaat ctgcaaaatg
     4501 ccaaaaggct ctgttcctgc cctgcaacag gaaatgctac gacgtgtcgg taagcgttat
     4561 gatgatgtag atgtgattgt gaaatctgcc agtaatgatg gactcacaat tttaagagcc
     4621 gtggataaag aatccgcgca agaatttgta caggaaacgc tgcaggaagt ctgggaaaca
     4681 gcggatgact ggtttatccg gtgacatcag cacggggagc aggcagctcc cctcttttat
     4741 tgtgttatca gtgcagcaag tacacagagt gttaccacac cacccagcca tacaaacagc
     4801 atgaagcaca tcgcacccgc tgtgtatttc cgctttccgc atctgatttt ttcctgctcc
     4861 attctcactg ccaccgttca actctcatta cccgacttca cgaatatgca ttccggctac
     4921 gcacgtccaa ccaggtgctt cacactcctg tacttaagac cgtttttacc aagtcccata
     4981 tacagaaagg tgttcaccgt cttcgtgttt atccccattt cacgggcaag atggcagggc
     5041 tttgactccc ggtacacgta tccaaacatc accaccatct cggacatcgt cagaggtcgc
     5101 gcatacgtat tatgtaccca ctcaccattc cggattttcc tgtacgtctc caggcgcccc
     5161 gtaacatctt ccgcatcaat ctgaagataa cggcgtccct gaaacatgca gcacatcagc
     5221 ctgccaagga gggcatcaca gattatcacc catgactcat catgtgccgg tacactcttc
     5281 ctgttctctg gtgtaaaaaa ctccctcgcc agtattatcc tgtacccctc aagcatctgc
     5341 tgcagaccat actccagata acggttgcgg gtcgccagaa tcactttcat cgctgccacc
     5401 tgtttcatgc tcacatgaga aaaaacggca ggcaaaactc cacgcctgcc gtcatgcttc
     5461 atgtcactgt cagcaattaa aactcaaagt tcacaccagc gttccaggat acatcgccat
     5521 cgctcgctgc cacacctgct ttcgctgctg tctgctcatt gaaacggtaa ccaacaccca
     5581 cggcaacagc ctgtttgtca ctgtaaccac caacggcagc agtggcgttg aatttaccca
     5641 cactgtacgg ctggaaaaga cctgccagcg ctgcgctctg ggctgccgcg cgtttcatct
     5701 cttcgtggtt ttcattaatc tgacgctgat gactgttcag acgtgtgctg tgctcctgca
     5761 actgacggga agtggactga atcgcctgtg tatttttagt aacccggcgg tcaacactac
     5821 caacagtagc atcaatacgg tctgttctgc cggacaaagc tacaagccca tcattaacag
     5881 acagggtttt gctggctcct tctgcacggg tcagggttcc gtcggagtta acaccaactg
     5941 tcgttctgga gtctgtatat tcacgaaact ttttcgcttc gaccacaagg cgggtattcg
     6001 tatcctgagc cagttgctca acattgtctt ttacacggtc aacctgacga actgttgctg
     6061 catctgaggg acgagtaccg tcactcagac caccaagcac ccgggtttta taagccttac
     6121 ccgactctgc ttcataaccg atataaacct cattgtcacg ggttgtgcgt gaattagctc
     6181 ctaatgcaac actgttttta tttgttactg ttgcctttgc ccctaacgca gtaccttctg
     6241 ttgcatcttt atcaactttg gctcggttac ctgctgccgt gccatatgta gccgtgctat
     6301 caacaaatgc aacgcttccc agggctgtag atgcatctgc ataagcatat gttgccccac
     6361 ctaaagccac actttgctgt cctcgtgctg cagaccatga tcccactgat gttgcaaatt
     6421 ttttatccgc gctggcccct gtaccaacag ctagcgcaat ctctccgtcc gcgcgagctg
     6481 aagcagaaag atctgtatgt tctgggatac tccctgaggc gataagctta ttctccgctg
     6541 catcctgagc cagaacgcca gcgcttacac caaataaaat accgccaata acaacggaaa
     6601 taacacttcg tggtaaaga
//
LOCUS       DAZFLD010000222         6587 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.223, whole genome shotgun sequence.
ACCESSION   DAZFLD010000222 DAZFLD010000000
VERSION     DAZFLD010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6587
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.223"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            26..928
                     /locus_tag="ACHKBL_004158"
     CDS             26..928
                     /locus_tag="ACHKBL_004158"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HFO5820947.1"
                     /translation="MIKKIFALPVIEQISPVLSRRKLDELDLIVVDHPQVKASFALQG
                     AHLLSWKPAGEEEVLWLSNNTPFKNGVAIRGGVPVCWPWFGPAAQQGPAAQQGLPAHG
                     FARNLPWTLKSHREDADGVALTFELTQSEETKKFWPHDFTLLAHFRVGKACEIDLEAH
                     GEFETTSALHTYFNVGDITKVSVSGLGDRFIDKVNDAKEDVLTDGIQTFPDRTDRVYL
                     NPQDCSVINDEALNRIIAVGHQHHLNVVGWNPGPALSVSMGDMPDDGYKTFVCVETAY
                     ASETQKVTKEKPAHLAQSIRVAKR"
     gene            complement(979..1833)
                     /gene="yeaE"
                     /locus_tag="ACHKBL_004159"
     CDS             complement(979..1833)
                     /gene="yeaE"
                     /locus_tag="ACHKBL_004159"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal reductase YeaE"
                     /protein_id="HFO5820948.1"
                     /translation="MQQKMIQFSGDVSLPAIGQGTWYMGEDASQRKTEVAALRAGIEL
                     GLTLIDTAEMYAEGGAEKVVGEALTGLRDNVFLVSKVYPWNAGGQKAINACEASLRRL
                     NTDYLDLYLLHWSGSFAFEETVAAMEKLIAQGKIRRWGVSNLDYADMQELWQLPGGNQ
                     CATNQVLYHLGSRGIEYDLLPWCQQQQIPVMAYSPLAQAGRLRNGLLKNAVVNEIAHA
                     HNISAAQVLLAWVISHQGVMAIPKAATIAHVQQNAAVLEVELSSAELAMLDKAYPAPK
                     GKTALDMV"
     gene            complement(1923..2669)
                     /gene="mipA"
                     /locus_tag="ACHKBL_004160"
     CDS             complement(1923..2669)
                     /gene="mipA"
                     /locus_tag="ACHKBL_004160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein MipA"
                     /protein_id="HFO5820949.1"
                     /translation="MTKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDT
                     DVYPVPVINYDGDNFWFRGLGGGYYLWNDATDKLSITAYWSPLYFKAKDSGDHQMRHL
                     DDRKSTMMAGLSYAHFTQYGYLRTTLAGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGI
                     GVQWNSENQNEYYYGVSRKESARSGLRGYNPNDSWSPYLELSASYNFLGDWSVYGTAR
                     YTRLSDEVTDSPMVDKSWTGLISTGITYKF"
     gene            3105..5039
                     /gene="yeaG"
                     /locus_tag="ACHKBL_004161"
     CDS             3105..5039
                     /gene="yeaG"
                     /locus_tag="ACHKBL_004161"
                     /EC_number="2.7.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011411173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YeaG"
                     /protein_id="HFO5820950.1"
                     /translation="MNIFDHYRQRYEAAKDEEFTLQEFLTTCRQDRSAYANAAERLLM
                     AIGEPVMVDTAQEPRLSRLFSNRVIARYPAFEEFYGMEDAIEQIVSYLKHAAQGLEEK
                     KQILYLLGPVGGGKSSLAERLKSLMQLVPIYVLSANGERSPVNDHPFCLFNPQEDAQI
                     LEKEYGIPRRYLGTIMSPWAAKRLHEFGGDITKFRVVKVWPSILQQIAIAKTEPGDEN
                     NQDISALVGKVDIRKLEHYAQNDPDAYGYSGALCRANQGIMEFVEMFKAPIKVLHPLL
                     TATQEGNYNGTEGISALPFNGIILAHSNESEWVTFRNNKNNEAFLDRVYIVKVPYCLR
                     ISEEIKIYEKLLNHSELTHAPCAPGTLETLSRFSILSRLKEPENSSIYSKMRVYDGES
                     LKDTDPKAKSYQEYRDYAGVDEGMNGLSTRFAFKILSRVFNFDHVEVAANPVHLFYVL
                     EQQIEREQFPQEQAERYLEFLKGYLIPKYAEFIGKEIQTAYLESYSEYGQNIFDRYVT
                     YADFWIQDQEYRDPDTGQLFDRESLNAELEKIEKPAGISNPKDFRNEIVNFVLRARAN
                     NSGRNPNWTSYEKLRTVIEKKMFSNTEELLPVISFNAKTSTDEQKKHDDFVDRMMEKG
                     YTRKQVRLLCEWYLRVRKSS"
     gene            5152..6435
                     /locus_tag="ACHKBL_004162"
     CDS             5152..6435
                     /locus_tag="ACHKBL_004162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaH/YhbH family protein"
                     /protein_id="HFO5820951.1"
                     /translation="MTWFIDRRLNGKNKSMVNRQRFLRRYKAQIKQSISEAINKRSVT
                     DVDSGESVSIPTEDISEPMFHQGRGGLRHRVHPGNDHFVQNDRIERPQGGGGGSGSGQ
                     GQASQDGEGQDEFVFQISKDEYLDLLFEDLALPNLKQNQQRQLTEYKTHRAGYTANGV
                     PANISVVRSLQNSLARRTAMTAGKRRELHALEENLAIISNSEPAQLLEEERLRKEIAE
                     LRAKIERVPFIDTFDLRYKNYEKRPDPSSQAVMFCLMDVSGSMDQSTKDMAKRFYILL
                     YLFLSRTYKNVEVVYIRHHTQAKEVDEHEFFYSQETGGTIVSSALKLMDEVVKERYNP
                     AQWNIYAAQASDGDNWADDSPLCHEILAKKILPVVRYYSYIEITRRAHQTLWREYEHL
                     QSTFDNFAMQHIRDQDDIYPVFRELFHKQNATAKD"
BASE COUNT         1730 a         1635 c         1603 g         1619 t
ORIGIN      
        1 taaaatgaag ttaaaggact gcgtcatgat taagaaaatt tttgcccttc cggtcatcga
       61 acaaatctcc cctgtcctct cccgtcgtaa actggatgaa ctggacctca ttgtggtcga
      121 tcatccccag gtaaaagcct cttttgcatt acagggcgca caccttctct catggaaacc
      181 tgcgggtgaa gaagaagttc tgtggttgag caataacaca ccgttcaaaa atggcgtcgc
      241 tattcgcggt ggcgtaccgg tttgctggcc gtggtttggt ccggctgcac aacaaggtcc
      301 ggctgcacaa caaggtctgc ctgcgcacgg ttttgcccgc aacctgccgt ggacgctgaa
      361 atcacatcgt gaagatgctg atggcgtagc gctgactttt gaattgacac aaagcgaaga
      421 gacgaaaaaa ttctggccgc acgactttac gctattagcg catttccgcg tgggtaaagc
      481 ctgtgaaatc gatcttgaag cacacggcga atttgaaacc acctctgccc tgcataccta
      541 ctttaacgtg ggtgatatca ctaaggtaag cgtcagtgga ctgggcgatc gcttcattga
      601 taaagtgaat gacgcgaaag aagatgtact gaccgacggt attcagacct tccctgaccg
      661 taccgatcgc gtgtatctga atccgcaaga ttgcagcgtg attaatgacg aagcgctgaa
      721 tcgtattatc gccgtaggcc accagcatca tctgaacgtc gttggctgga acccaggacc
      781 ggcgctttca gttagcatgg gcgatatgcc ggacgatggc tacaaaacct ttgtttgtgt
      841 agaaacggct tacgcttcag aaacgcaaaa agtgaccaaa gaaaaacctg cacatctggc
      901 gcaatccatt cgcgttgcga aacgttaatt tgcgttaatg ttgtgtgccg ggtgcgttgc
      961 atccggcacg tatcaacatc acaccatatc cagcgcagtt tttccttttg gtgccggata
     1021 tgccttatcc agcatagcta attccgctga cgaaagttca acctcaagca cagccgcatt
     1081 ttgttggaca tgggcaatcg tggccgcttt tggaatcgcc atcacaccct gatgactgat
     1141 cacccacgcc aacaatactt gtgccgcgct gatattgtga gcatgtgcaa tttcgttgac
     1201 tactgcgttt tttagcagcc cattgcgcaa ccgcccggcc tgagctaacg gactgtaagc
     1261 catcaccggt atctgctgtt gctggcacca ggggagtaga tcgtactcaa tccctcgtga
     1321 accgagatgg taaagcacct gattagtggc acactgattt ccccccggca gctgccagag
     1381 ttcctgcatg tcggcataat caaggttaga aacgccccag cggcggattt ttccctgggc
     1441 gatcaatttt tccatcgctg cgacagtctc ttcaaaagcg aaactgccag accagtgtaa
     1501 taagtagaga tcgagataat cagtattgag acggcgtaaa ctggcttcgc atgcatttat
     1561 cgctttttgc ccgccagcat tccacggata gactttagag acgagaaaga cgttatcacg
     1621 cagaccggtt aatgcttccc caaccacctt ttcagcaccg ccttcggcat acatttcggc
     1681 ggtatcaatg agggttaaac cgagttcaat gcccgcgcgt agtgcagcaa cttctgtttt
     1741 gcgctgactg gcatcttcgc ccatatacca tgttccctgc cctatggctg gcagtgagac
     1801 atcgccacta aattgaatca ttttttgttg cattgtttcc tcccaggtat tgcaccaccg
     1861 taatgcaaaa cagggcatga cgccctgttt ttatgcacaa aatgccctgg aaagatgcat
     1921 tatcagaatt tgtaggtgat cccggtagaa atcaggccag tccaggattt atccaccatt
     1981 gggctgtcag taacttcatc agacagacgg gtgtagcgcg cggtaccgta aacactccag
     2041 tcgccgagga agttgtagct ggcgctcagc tccaggtaag ggctccagct gtcgttcggg
     2101 ttatagccac gcagaccgct gcgagcggac tctttgcgcg atacgccata atagtattcg
     2161 ttctggtttt cgctgttcca ctgcacacca atacccggag tcacggtcag gccaccgttg
     2221 gtgtaacgat acaaccaggc catatcccag acgatgccgt tgctgttatc cagggtatcg
     2281 ccagccaggg tggtacgcag gtaaccgtac tgggtaaagt gagcataaga cagaccagcc
     2341 atcatggtgc tcttacggtc atccaggtga cgcatttggt gatcgccact gtctttagct
     2401 ttgaagtaaa gcggcgacca gtaagcggta attgaaagtt tgtccgttgc gtcattccac
     2461 aggtagtaac caccacctaa gccacggaac cagaagttat cgccgtcata gttgattacc
     2521 ggtactgggt aaacatcggt atcgtaatct ttatatgggt gttcaacgac acctacgcct
     2581 gcgcccaggg aaaatttacc ttcagcgtgc gctacgcctg cagacgttgc gataagcact
     2641 ccaagtgcca gaagtttgag tttggtcaca attaatcatt ccttaaacaa atgtttagcg
     2701 ggcgacaaag tttacccgtc aatacatcga aacccaacct ttttacgttt tcatttttta
     2761 tagtaactgt ttaattttcc tgacgcagat gacaccactc ttacagccaa atgaatttag
     2821 cgttaccggc gaccctggta tttacattaa ttatgcaaaa tttatggatg agttgttgat
     2881 atgccattga aattaagaaa gccgtgcagg caagttttcc atttgccatc tacgcttaat
     2941 tttgaaggtg tatcaccggg cacgttgttc tcatcgtcga taaaatggca tgagagttgc
     3001 tgtgttttag caagagacgt cgttcagttt acttcttccg ggagcctcta ctattcatat
     3061 gaacggctct taacctgtgc taaaaaacga aaggacggca taccatgaat atattcgatc
     3121 actatcgcca gcgatatgaa gctgccaagg acgaagagtt cacactgcag gagtttctta
     3181 ccacttgtcg gcaagatcgc agtgcttatg ccaacgcggc tgagcggcta ttgatggcta
     3241 tcggtgagcc tgtcatggtc gatacagccc aggaacccag actttctcga ctcttttcta
     3301 accgggtcat agcacgttat ccggcatttg aagagtttta cggcatggaa gacgcgattg
     3361 aacagattgt ctcttatctg aaacacgcgg ctcaggggct ggaagagaag aaacaaatcc
     3421 tgtatctgct tgggcctgtg ggtgggggta aatcatcgct tgctgagcga ctgaaatcat
     3481 taatgcagct cgtaccgatt tatgtattga gcgccaacgg tgagcgtagc ccggtcaacg
     3541 atcatccgtt ctgtcttttc aatccgcagg aagatgcgca gattctggaa aaagagtatg
     3601 gcattcctcg ccgttatctc ggcaccatca tgtcgccgtg ggcggcaaaa cgcctgcatg
     3661 aatttggtgg cgatatcact aagttccggg tagtgaaggt ctggccgtca attctgcaac
     3721 aaattgctat cgccaaaacg gaacccggtg atgagaacaa ccaggacatc tccgcgctgg
     3781 ttgggaaagt cgatattcgt aaactcgaac actacgcgca gaatgacccg gacgcctacg
     3841 gctattccgg tgcactgtgc cgcgccaacc aggggatcat ggaattcgtt gagatgttta
     3901 aagcaccgat taaagtgctg catcccttgc taaccgccac tcaggaaggt aactacaacg
     3961 ggacggaagg tatctccgcc ctgccgttca acgggattat tctcgcccac tcgaacgaat
     4021 ccgaatgggt cactttccgt aataacaaaa acaacgaagc cttcctcgat cgtgtttaca
     4081 tcgtgaaggt gccgtattgc ttgcgcattt ccgaagagat caaaatctac gagaaattgc
     4141 ttaatcacag tgaattgact cacgccccct gcgcccctgg cacgctggaa acgctgtcac
     4201 gtttttccat tctttcgcgc cttaaagagc cagaaaactc cagcatttat tcaaagatgc
     4261 gggtttatga tggcgaaagt ctgaaagaca cagatcccaa agccaagtcg tatcaggaat
     4321 atcgtgacta cgccggtgtc gatgaaggga tgaacggtct gtcgacgcgt tttgcgttta
     4381 agatcctctc ccgcgtgttc aacttcgatc atgtagaagt ggcggcaaac ccggttcatc
     4441 tgttctacgt cctggaacag cagatcgagc gcgagcagtt cccgcaagaa caggcagaac
     4501 gctatctgga gttcctgaaa ggttatctga tcccgaaata tgccgagttt atcggcaaag
     4561 agatccagac ggcctacctt gaatcctatt ccgaatatgg gcaaaacatt ttcgaccgtt
     4621 atgttaccta cgcggatttc tggattcagg atcaggagta tcgcgatcct gataccgggc
     4681 agttgtttga ccgcgagtct cttaacgccg agctggagaa aatcgagaaa ccggcgggga
     4741 tcagtaatcc aaaagatttc cgcaacgaga ttgttaactt cgtactgcgc gccagagcga
     4801 ataacagcgg acgcaatccg aactggacca gctatgaaaa actgcgcacg gttattgaga
     4861 agaaaatgtt ctccaatacc gaggagctgt tgccggttat ttcgtttaac gccaaaacgt
     4921 caaccgacga gcaaaagaaa catgacgact ttgtcgaccg tatgatggaa aaaggctaca
     4981 cccgtaaaca ggtgcgttta ctgtgcgaat ggtatttgcg cgtacgtaaa tcgtcttaac
     5041 aaccctggcc cggtacacat gctaccgggc ctacaacgac agcgaaccgt gggccagaga
     5101 agcggcaaca caggcgtagc ataaagttgg caaatgtagt acggggggca tatgacctgg
     5161 tttattgacc ggcgtctgaa cggcaaaaac aaaagcatgg tgaatcgcca gcgtttttta
     5221 cgccgttata aagcgcaaat taaacagtcg atctccgagg ccattaataa gcgttcggtg
     5281 actgacgtcg acagcggcga gtccgtatcc attcccacgg aagatattag cgaaccgatg
     5341 tttcatcagg ggcgtggcgg tctgcgccac cgcgtgcatc cgggcaatga ccatttcgtc
     5401 cagaacgacc gaattgaacg tccccagggt ggcggcggag gttccggcag cggtcagggc
     5461 caggccagcc aggatggtga aggtcaggat gaattcgtct ttcagatttc gaaagatgag
     5521 tatcttgatc tgctctttga agatttggct ttaccgaatc tgaaacaaaa ccaacaacgc
     5581 cagctgaccg aatataaaac gcatcgggcg ggttataccg ctaacggcgt tccggccaat
     5641 atcagcgttg tgcgttcatt gcagaactca ctggcgcggc gcacagccat gacggcgggc
     5701 aagcggcggg aacttcatgc gctggaagag aatttggcca tcatcagcaa cagtgaacct
     5761 gcgcaactgc tggaagagga acgtctgcgc aaagaaattg cagaattacg tgccaaaatt
     5821 gaacgcgtcc ctttcattga caccttcgat ttacgttaca agaactacga aaaacggccc
     5881 gatccctcca gccaggcagt gatgttttgc ctgatggacg tttccggttc aatggatcag
     5941 tccactaaag atatggctaa acgtttttat attctgctgt atctgttcct cagcagaaca
     6001 tataagaacg tggaagtcgt atacatccgc catcataccc aggcgaaaga agtcgatgaa
     6061 catgagtttt tctactcgca ggaaacaggc ggcaccattg tttccagcgc cctgaaactg
     6121 atggatgagg tagtgaaaga gcgttataac ccggcacagt ggaatattta cgcagcccaa
     6181 gcatcagacg gcgataactg ggccgatgac tctccgcttt gccatgaaat tctggcgaaa
     6241 aaaatattac ccgttgttcg ttattacagc tatatcgaaa ttacacgtcg cgcgcatcag
     6301 acactgtggc gagaatatga gcatctgcaa tctactttcg acaactttgc gatgcagcat
     6361 atccgcgacc aggatgatat ttatccggtg ttccgtgaac tgtttcataa acaaaatgca
     6421 acagctaaag actaaaactg tcagccgggt cattatcgcc tggctgattt ttagttcact
     6481 gtaaattatc tcatttatta catacattag cttacaatcg ctttaaatat gacagcataa
     6541 cctttacaca atttagttcc agaaaacaat cattcggaaa aatgatt
//
LOCUS       DAZFLD010000223         6549 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.224, whole genome shotgun sequence.
ACCESSION   DAZFLD010000223 DAZFLD010000000
VERSION     DAZFLD010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6549
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.224"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(171..2204)
                     /gene="betT"
                     /locus_tag="ACHKBL_004163"
     CDS             complement(171..2204)
                     /gene="betT"
                     /locus_tag="ACHKBL_004163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline BCCT transporter BetT"
                     /protein_id="HFO5820952.1"
                     /translation="MTDLSHSREKDKINPVVFYTSAGLILLFSLTTILFRDFSALWIG
                     RTLDWVSKTFGWYYLLAATLYIVFVVCIACSRFGSVKLGPEQSKPEFSLLSWAAMLFA
                     AGIGIDLMFFSVAEPVTQYMQPPEGAGQTIEAARQAMVWTLFHYGLTGWSMYALMGMA
                     LGYFSYRYNLPLTIRSALYPIFGKRINGPIGHSVDIAAVIGTIFGIATTLGIGVVQLN
                     YGLSVLFDIPDSMAAKAALIALSVIIATISVTSGVDKGIRVLSELNVALALGLILFVL
                     FMGDTSFLLNALVLNVGDYVNRFMGMTLNSFAFDRPVEWMNNWTLFFWAWWVAWSPFV
                     GLFLARISRGRTIRQFVLGTLIIPFTFTLLWLSVFGNSALYEIIHGGAAFAEEAMVHP
                     ERGFYSLLAQYPAFTFSASVATITGLLFYVTSADSGALVLGNFTSQLKDINSDAPGWL
                     RVFWSVAIGLLTLGMLMTNGISALQNTTVIMGLPFSFVIFFVMAGLYKSLKVEDYRRE
                     SANRDTAPRPLGLQDRLSWKKRLSRLMNYPGTRYTKQMMETVCYPAMEEVAQELRLRG
                     AYVELKSLPPEEGQQLGHLDLLVHMGEEQNFVYQIWPQQYSVPGFTYRARSGKSTYYR
                     LETFLLEGSQGNDLMDYSKEQVITDILDQYERHLNFIHLHREAPGHSVMFPDA"
     gene            2333..2920
                     /gene="betI"
                     /locus_tag="ACHKBL_004164"
     CDS             2333..2920
                     /gene="betI"
                     /locus_tag="ACHKBL_004164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308386.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BetI"
                     /protein_id="HFO5820953.1"
                     /translation="MPKLGMQSIRRRQLIDATLEAINEVGMHDATIAQIARRAGVSTG
                     IISHYFRDKNGLLEATMRDITSQLRDAVLNRLHALPQGSAELRLQAIVGGNFDETQVS
                     SAAMKAWLAFWASSMHQPMLYRLQQVSSRRLLSNLVSEFRRELPRQQAQEAGYGLAAL
                     IDGLWLRAALSGKALDKPLAHSLTRHFITQHLPTD"
     gene            2934..4406
                     /gene="betB"
                     /locus_tag="ACHKBL_004165"
     CDS             2934..4406
                     /gene="betB"
                     /locus_tag="ACHKBL_004165"
                     /EC_number="1.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008802 - betaine-aldehyde dehydrogenase
                     (NAD+) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019285 - glycine betaine biosynthetic
                     process from choline [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="betaine-aldehyde dehydrogenase"
                     /protein_id="HFO5820954.1"
                     /translation="MSRMAEQQLYIHGGYTSATSGRTFETINPANGNVLATVQAAGRE
                     DVDRAVKSAQQGQKIWAAMTAMERSRILRRAVDILRERNDELAKLETLDTGKAYSETS
                     TVDIVTGADVLEYYAGLIPSLEGSQIPLRETSFVYTRREPLGVVAGIGAWNYPIQIAL
                     WKSAPALAAGNAMIFKPSEVTPLTALKLAEIYSEAGLPDGVFNVLPGVGAETGQYLTE
                     HPGIAKVSFTGGVASGKKVMANSAASSLKEVTMELGGKSPLIVFDDADLDLAADIAMM
                     ANFFSSGQVCTNGTRVFIPAKCKAAFEQKILARVERIRAGDVFDPQTNFGPLVSFPHR
                     DNVLRYIAKGKEEGARVLCGGDVLKGDGFDNGAWVAPTVFTDCRDEMTIVREEIFGPV
                     MSLLTYESEDEVIRRANDTDYGLAAGIVTADLNRAHRVIHQLEAGICWINTWGESPAE
                     MPVGGYKHSGIGRENGVMTLQSYTQVKSIQVEMAKFQSIF"
     gene            4420..6108
                     /gene="betA"
                     /locus_tag="ACHKBL_004166"
     CDS             4420..6108
                     /gene="betA"
                     /locus_tag="ACHKBL_004166"
                     /EC_number="1.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008812 - choline dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0019285 - glycine betaine biosynthetic
                     process from choline [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="choline dehydrogenase"
                     /protein_id="HFO5820955.1"
                     /translation="MQFDYIIIGAGSAGNVLATRLTEDPNTTVLLLEAGGPDYRFDFR
                     TQMPAALAFPLQGKRYNWAYETEPEPFMNNRRMECGRGKGLGGSSLINGMCYIRGNAL
                     DLDNWAKEPGLENWSYLDCLPYYRKAETRDVGENDYHGGDGPVSVTTSKPGVNPLFEA
                     MIEAGVQAGYPRTDDLNGYQQEGFGPMDRTVTPQGRRASTARGYLDQAKSRPNLTIRT
                     HAMTDHIIFDGKRAVGVEWLEGDSTIPTRAAANKEVLLCAGAIASPQILQRSGVGNAE
                     LLAEFDIPLVHDLPGVGENLQDHLEMYLQYECKESVSLYPALQWWNQPKIGAEWLFGG
                     TGVGASNHFEAGGFIRSREEFAWPNIQYHFLPVAINYNGSNAVKEHGFQCHVGSMRSP
                     SRGHVRIKSRDPHQHPAILFNYMSHEQDWQEFRDAIRITREIMHQPALDQYRGREISP
                     GAECQTDEQLDEFVRNHAETAFHPCGTCKMGYDEMAVVDAEGRVHGLEGLRVVDASIM
                     PQIITGNLNATTIMIGEKMADMIRGQEALPRSTARYFVANGMPVRAKKMSRDVN"
BASE COUNT         1600 a         1801 c         1795 g         1353 t
ORIGIN      
        1 acaacagtcc aaacgtaggt tttgggcttc aatagttctt ttctttttta ttatctgttg
       61 cctggcaatc atcaatctat actaagggct taactgtaca gatataacgt atcaagaggt
      121 aagagaaacc gcgccatatg catacatggc gcggttttat gcaataacaa tcacgcgtcc
      181 gggaacatca cgctatggcc cggcgcttca cgatggagat gaataaagtt aaggtgccgc
      241 tcgtactggt caagaatatc ggtgatcacc tgctctttgc tgtagtccat caggtcgttg
      301 ccctggctgc cttctaacag gaaggtttcc agccggtagt aggtcgattt accgctacgt
      361 gcgcggtagg taaagcccgg caccgaatat tgctgcggcc aaatctgata gacaaagttt
      421 tgttcttcgc ccatatgcac caacaaatcc agatgcccca actgttgtcc ctcttccggc
      481 ggcaggcttt ttagctccac gtatgcgccg cgcaaccgca gctcctgcgc cacttcttcc
      541 attgccgggt aacagaccgt ctccatcatc tgtttagtgt aacgcgtgcc cggataattc
      601 atcaggcgcg agagacgttt tttccagctc aggcgatcct gaagccccag cggtcgcggc
      661 gcggtatcgc ggttcgcact ttcacggcgg taatcttcta ccttcagaga tttatacaac
      721 cccgccatca cgaagaaaat cacaaagctg aacggcagcc ccatgattac cgtggtgttt
      781 tgcagcgcgg atatcccgtt ggtcatcagc atgccgagcg tcagcaggcc aatcgccacc
      841 gaccagaaga cgcgcagcca gccgggggcg tcgctgttga tatctttaag ctgcgaggtg
      901 aaattcccca gcaccagcgc accggagtcc gccgaggtca cataaaacag caggccagta
      961 atggtggcga cggaggcgct aaaggtaaac gccggatatt gcgccagcag gctgtagaag
     1021 ccgcgctccg gatggaccat cgcttcctcg gcaaatgccg cgccgccgtg gatgatttca
     1081 tacagcgcgc tattgccgaa taccgagagc cataacagcg tgaaggtaaa cggaataatc
     1141 aacgtgccca gcacgaactg gcgaatggta cgcccacgcg agatacgcgc caggaacaag
     1201 ccgacaaacg gcgaccatgc cacccaccat gcccagaaga agagcgtcca gttattcatc
     1261 cactcaaccg gacggtcgaa ggcaaaactg ttgagcgtca tgcccataaa gcgattcaca
     1321 tagtcgccaa cattcagcac cagtgcatta agcaggaacg aggtgtcgcc cataaacaac
     1381 acgaacagga tcaatcccag cgccagcgca acgttgagtt ccgataacac gcgaatgccc
     1441 ttatcaacac cggatgtcac cgagatagtg gcgatgatca ccgacaaggc gatcagtgcc
     1501 gctttcgccg ccatcgaatc gggaatatca aacagtacgc tcaagccata gttaagctgc
     1561 accacaccga taccgagcgt agtggcaata ccgaagatgg tgccgatcac cgctgcaata
     1621 tccactgaat gaccaatcgg cccgttaatc cgtttaccga agatcgggta cagcgccgag
     1681 cggatggtga gcggcaaatt ataacgataa ctaaagtatc cgagcgccat gcccatcagc
     1741 gcatacatcg accagccggt taagccgtag tgaaacagcg tccagaccat cgcctgacgc
     1801 gcggcctcaa tcgtctgtcc cgcgccttcc ggcggctgca tatactgcgt taccggttcg
     1861 gctacggaga agaacatcag gtcgataccg atcccggcag caaacagcat cgccgcccaa
     1921 ctcagcagac tgaattccgg tttggattgt tctggcccga gcttcaccga accaaaacgc
     1981 gaacaagcga tacagaccac aaagacaata tagagcgttg ccgccagcag atagtaccaa
     2041 ccgaaggttt tagaaaccca gtccagcgtg cggccaatcc acagggccga gaagtcgcga
     2101 aacaggatcg ttgtcaggga aaacaacaaa atcagtccgg cggaggtgta aaacaccacc
     2161 ggattgattt tgtccttttc cctgctgtgt gaaaggtctg tcatccagta tccccactgt
     2221 tattgttacc atttaaaatc aaattcgtaa caattaagac acattttata ttgaacgtcc
     2281 aatcaataac cgctttaata gataaacaac gctgatgaat ggagtggcga aaatgcccaa
     2341 attggggatg cagtcgatcc ggcgcagaca actgatcgac gccacgctgg aagcaataaa
     2401 tgaagtgggc atgcacgatg caacgatcgc gcagatcgcc cgccgtgcag gcgtttctac
     2461 ggggatcatc agccactatt tcagggacaa aaatggtctg ctggaagcaa ccatgcgcga
     2521 tatcaccagt cagctgcgtg acgcggtttt gaatcgatta catgcacttc cgcagggcag
     2581 tgcagagctg cgattacagg cgattgttgg tggaaacttc gatgaaaccc aggtgagcag
     2641 tgcggcgatg aaagcctggc tggccttctg ggccagcagt atgcatcagc cgatgctcta
     2701 tcgcttgcag caggtcagta gccgccgctt attgtcgaat ctggtgagcg aatttcgccg
     2761 cgagttaccg cgccaacagg cacaggaagc gggctacggc cttgccgcgc tgattgacgg
     2821 attatggctg cgcgcggcac tgagcggcaa agcgctggat aaacctctcg cacactcgct
     2881 gacccgtcac tttattactc agcatttacc caccgattaa ccgaggaaac gtgatgtccc
     2941 gaatggcaga acagcagctt tatatacatg gtggttatac ctccgccacc agcggtcgca
     3001 ccttcgagac cattaacccg gccaacggta acgtgctggc gaccgtgcag gccgccgggc
     3061 gtgaggatgt cgatcgcgcc gtgaagagtg cccaacaagg gcaaaaaatc tgggcggcga
     3121 tgaccgctat ggagcgctcg cgtattctgc gtcgggccgt tgatattctg cgtgaacgca
     3181 atgacgaact cgcaaaactg gaaaccctcg acaccggaaa agcgtattcg gaaacctcaa
     3241 ccgtcgatat cgttaccggt gcggacgtgc tggagtacta cgccgggctg atcccgtcgc
     3301 tggaaggcag ccagatccca ttgcgtgaga cgtcatttgt ttatacccgc cgcgaaccgc
     3361 tgggcgtagt ggcagggatt ggcgcatgga actacccgat ccagattgcc ttgtggaaat
     3421 ccgccccggc gctggcggca ggcaacgcga tgattttcaa accgagcgaa gtcaccccgc
     3481 ttaccgcgtt aaagctggcc gaaatttaca gcgaagcggg cctgccggac ggcgtattta
     3541 acgtgctgcc gggcgtaggt gcggaaaccg ggcagtatct gaccgagcat ccgggcattg
     3601 ccaaagtgtc atttaccggc ggtgtcgcca gcggcaaaaa agtgatggct aactcggcgg
     3661 cttcctccct gaaagaagtg accatggagc tgggcggtaa atcaccgctg atcgttttcg
     3721 acgatgcgga tctcgatctc gccgctgata tcgccatgat ggcgaacttc ttcagctccg
     3781 gtcaggtgtg taccaacggc acccgcgtgt ttattccggc gaaatgcaaa gccgcatttg
     3841 aacaaaagat tctggcgcgc gttgagcgca ttcgcgcggg cgacgttttc gatccgcaaa
     3901 ctaactttgg cccgctggtc agcttcccgc atcgcgataa cgtgctgcgt tacatcgcca
     3961 aaggcaaaga ggaaggcgcg cgcgtactgt gcggcggcga tgtactgaaa ggcgatggct
     4021 tcgataacgg cgcatgggtt gcaccgaccg tgttcaccga ttgccgcgac gaaatgacca
     4081 tcgtgcgcga agagatcttc ggtccggtga tgtcgcttct cacttatgaa tctgaggacg
     4141 aagtgattcg ccgcgccaat gataccgact acggcctggc ggcgggcatc gtgacggcgg
     4201 acctgaaccg cgcgcatcgc gtcattcatc agctggaagc gggtatttgc tggatcaaca
     4261 cctggggcga atcccctgca gagatgcccg ttggcggcta caaacactcc ggcattggtc
     4321 gcgagaacgg cgtgatgacg ctccagagtt acacccaggt gaagtccatc caggttgaga
     4381 tggctaaatt ccagtccata ttctaaccag gaggtttatt tgcaatttga ctacatcatt
     4441 attggtgccg gctcagctgg caacgttctc gctacccgtc tgactgaaga tccgaacaca
     4501 accgtgctgc tgcttgaagc aggcggcccg gactatcgct ttgacttccg cacccagatg
     4561 cccgccgcgc tggcgttccc gttacagggt aaacgctata actgggccta tgagacggaa
     4621 ccggaaccgt ttatgaacaa ccgtcgcatg gagtgcggac gcggtaaggg gcttggcggg
     4681 tcatcactga tcaacggcat gtgttacatc cgtggtaacg cgctggatct cgataactgg
     4741 gcgaaagaac ccggtctgga gaactggagc tacctcgact gtctgcccta ctatcgcaag
     4801 gccgagactc gcgatgtggg cgagaacgac taccacggcg gtgatggccc ggtgagcgtc
     4861 accacctcca aacccggcgt caatccgctg tttgaagcga tgattgaagc gggcgtgcag
     4921 gcgggctacc cgcgcacgga cgatctcaac ggctatcagc aggaaggttt tggcccgatg
     4981 gatcgcaccg tcacgccgca gggccgtcgc gccagcaccg cgcgtggcta tctcgatcag
     5041 gccaaatcgc gccctaacct aaccattcgt actcacgcca tgaccgatca catcattttt
     5101 gacggcaaac gcgcggtggg cgtcgagtgg ctggaaggcg acagcaccat tccgacccgc
     5161 gcggcggcga ataaagaagt gctgttatgt gcaggcgcga ttgcttcacc gcagatcctg
     5221 caacgctccg gcgtcggcaa cgctgaactg ctggcggagt ttgatattcc gctggtgcat
     5281 gatttacccg gcgtcggcga aaatcttcag gatcacctgg agatgtatct gcaatatgag
     5341 tgcaaagaat cggtttccct ctaccctgcc ctgcagtggt ggaatcagcc gaaaatcggt
     5401 gcggagtggc tgtttggcgg caccggcgtt ggtgccagca accactttga agcaggcgga
     5461 tttattcgca gccgtgagga atttgcgtgg ccgaatattc agtaccattt cctgccagtg
     5521 gcgattaact ataacggctc gaacgcagtg aaagagcacg gcttccagtg ccacgtcgga
     5581 tcgatgcgct cgccaagccg tgggcatgtg cggattaaat cccgcgaccc gcaccagcat
     5641 ccagcgattc tgtttaacta catgtcgcac gagcaggact ggcaggagtt ccgcgacgca
     5701 attcgcatca cccgggagat catgcatcaa ccggcgctgg atcagtatcg tggccgcgaa
     5761 atcagccccg gcgcggaatg tcagacggat gagcagctcg atgagttcgt gcgtaaccac
     5821 gccgaaaccg cgtttcatcc gtgcggtacc tgcaaaatgg gttatgacga gatggcagtg
     5881 gttgacgccg aaggccgcgt acacgggctg gaaggcctgc gcgtggtgga tgcgtcaatt
     5941 atgccgcaaa ttatcaccgg caatttgaac gctacgacga ttatgattgg cgagaaaatg
     6001 gcggatatga ttcgcgggca agaagcgttg ccgaggagca cggcaagata ttttgtggca
     6061 aatgggatgc cggtgagagc gaaaaaaatg agtcgtgatg tgaactaact caggaaccta
     6121 tcagattgag agttaccgtt ctactatgag gaccgaatcc ttatataaaa cactgaggta
     6181 actctcatgc ttcatatccg cagtcccgcc atcaaacttt aagccgattc gctcaatctg
     6241 gcggatgatg gtgctatgga tacgctgata aaccacaagt gtaatgccac tcagcttaag
     6301 aatcacgcaa ccagtgctcc tggctagggt tacttgttct ttcccactcc cggttagcag
     6361 tggtcagcga tcaactgcgt aagccgggcg tatttatccc aaagcgtgga gtaatatcag
     6421 gtctacgcta aaaaagtcaa tttattcggt tatcatgcac gaaaagggga cttgtggcga
     6481 gtataaaaat aagtcagccc caaaaactac gtggagcttt aaacacggtt cgtataattt
     6541 attagcagc
//
LOCUS       DAZFLD010000224         6499 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.225, whole genome shotgun sequence.
ACCESSION   DAZFLD010000224 DAZFLD010000000
VERSION     DAZFLD010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6499
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.225"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..3649)
                     /locus_tag="ACHKBL_004167"
     CDS             complement(<1..3649)
                     /locus_tag="ACHKBL_004167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAAR domain-containing protein"
                     /protein_id="HFO5820956.1"
                     /translation="MSEGPGGPQGATAGGTLAMRMLSQQAMVASQMKRAANDKAIAQM
                     LAAKKSGPPAARLGDEIQHKSFLGALAGAVLGAIVTIAEGCLIMAACATGPYALVLVP
                     ALMYASYKASDYVEEKQNQLESWINSFCDTDGAINTGSENVKINGKPAARAAVTLPPP
                     PLTEAIPSPPQGEPSWGDIATDLLESAAEKAVPLAKAWGNAVITLTDSNAGIMDRVSA
                     GASLLFPAGPVLMEFATMVGGRGEIKKDVDFPEAGEDTALCDKENKPPRIAQGSSNVF
                     INNQPAARKGDKLECSAAIVEGSPDVFIGGEQVTYLDIQPEFPPWQRMILGGITIASY
                     LLPPAGLLGKLGNLAKLGKLGNLLGKSGKLLGAKLGALLGKTGKSLKSIANKVIRWVT
                     DPVDPVTGAYCDERTDFTLGQTLPLSFTRFHSSVLPLHGLTGVGWSDSWSEYAWVREQ
                     GNRVDIISQGATLYFAFDGDSDTAVNPYHAQYILRRRDDYLELFDRDALSSRFFYDAF
                     PGMRLRHPVTDDTNDDRLAHSPGDRMYMLGGMSDTASNRITFERDSQYRITGVSHTDG
                     IRLKLTYHASGYLKAIHRTDNGIQTLATYEQDARGRLTEADARLDYHLFYEYDAADRI
                     IRWSDNDQTWSRFTYDEQGRCVNVTGADGYYNATLDYSDGCTTVTDGKGIHRYYYDPD
                     GNILREEAPDGSTTTYEWDEFHHLLARHSPAGRVEKFEYNAVHGQLSRYTAADGAVWL
                     YRYDERGLLSNITDPAGQTWTQQCDERGLPVSLVSPQGEETRLAYTAQGLLSGIFRQD
                     ERRLGIEYDHHNRPETLTDVMGREHHTEYSGHDLPVKMRGPGGQSVRLQWQQHHKLSG
                     LERAGTGAEGFRYDRHGNLLAWTDGNGVDWTMEYGPFDLPVARTDGEGHRWQYRYDKD
                     TLQLTEVINPQGESYRYLLDNCGRVTEEHDWGGVTWRYRYDADGLCTARVNGLEETIL
                     YSRDAAGRLAEIITPEGKTQYAYDKSGRLTGIFSPDGTSQRTGYDERGRVNVTTQGRR
                     AIEYHYPDEHTVIRCILPPEDERDRHPDESLLKTTYRYNAAGELTEVILPGDETLTFS
                     RDEAGREVLRHSNRGFACEQGWNAAGQPVSQRAGFFPEEATWGGLVPSLVREYRYDSA
                     GNVSGVTSREDYGRETRREYRLDRNGQVTAVTASGTGLGYGEGDETYGYDSCGYLKAQ
                     SAGRHRISEETE"
     gene            complement(3669..4130)
                     /locus_tag="ACHKBL_004168"
     CDS             complement(3669..4130)
                     /locus_tag="ACHKBL_004168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DcrB-related protein"
                     /protein_id="HFO5820957.1"
                     /translation="MQFTFNEGHIQLPSQWQDQSMQVLVSTDNSGINLVITREAVPQG
                     TLTPELYQETLALYQGKLDGYTEHACREITLAEAPAWLLDYSWNGPEDEGNQGRIDQI
                     AVFQRRGDTLLTFTFSTSLSLKNSQKTMLLEVIKSFTPLPPENDIQKDQPR"
     gene            complement(4158..6059)
                     /locus_tag="ACHKBL_004169"
     CDS             complement(4158..6059)
                     /locus_tag="ACHKBL_004169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HFO5820958.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLTQSLSSLFSLDLSLVSQQ
                     FLSLEFAQVLDKMAYLTVWQGDDVQRRVKGVVTWFELGENDKNQMLYSMKVRPPLWRA
                     GLRQNFRIFQNEDIRSILGTILQENDVTEWSPLFSEPHPSREFCVQYGETDYDFLCRI
                     AAEEGIFFYEEHAQKSTDQSLVLCDTVRYLPESFEIPWNPNTRTEVSTLCISQFRYSA
                     QIRPSSVVTKDYTFKRPGWAGRFDQEGQHQDYQRTQYEVYDWPGRFKSAHGQNFARWQ
                     MDGWRNNAETARGMSRSPEIWPGRRIVLTEHPQANLNREWQVVASELHGEQPQAVPGR
                     RGSGTTLNNHFAVIPADRTWRPQPKPKPSVDGPQSAVVTGPAGEEIFCDEHGRVRVKF
                     NWDRYNPANQDSSCWIRVAQAWAGPGFGNLAIPRVGQEVIVDFLNGDPDQPIIMGRTY
                     HEDNRSPGSLPGTKTQMTIRSKTYMGSGFNELKFDDATGKEQVYIHAQKNMDTEVLND
                     RTTTVKHDHRETVKNDQTVMIQEGNRLLTVEKGHKITGVLKGSLSEDVFQDRGTIAGS
                     VHVDAVNNGGEGDGIQAYTAIKQIMLAVEESKIALTPDGIQLQVGESTVIRLSKDGIT
                     IVGGSVFIN"
BASE COUNT         1230 a         1942 c         1772 g         1555 t
ORIGIN      
        1 gctcagtctc ttcacttatc cggtgcctgc ccgcagactg cgccttcagg tagccgcagc
       61 tgtcatagcc ataagtctca tcgccttcgc catagcccag cccggtgcct gaggctgtca
      121 ccgccgtgac ctggccgttc cggtccagcc ggtactcccg gcgtgtttcc cgtccgtaat
      181 cttcccggct ggtgacacct gacacgttac ccgcgctgtc gtaacggtac tcccgtacca
      241 gtgacggaac cagcccgccc catgtggctt cttccggaaa aaatccggcg cgctggctga
      301 caggctggcc ggctgcattc cagccctgct cacaggcaaa accccggtta ctgtgccgga
      361 gcacttcacg tcctgcctca tcacggctga acgtcagcgt ctcatccccc ggcaggataa
      421 cctccgtcag ttctccggcg gcgttgtagc ggtaggtggt tttcagcagg gattcatcgg
      481 ggtgtctgtc gcgttcatct tccggtggca ggatacagcg gatgacggtg tgttcatcgg
      541 ggtaatggta ctcaatggcc cgtcggccct gagtggtgac attcacccgc ccgcgttcgt
      601 catagccggt gcgctgtgat gtgccgtccg ggctgaagat acccgtcagc ctgccggatt
      661 tgtcatacgc atactgcgtt ttgccttccg gcgtgatgat ttctgccagg cggcctgcgg
      721 cgtcccggct gtagaggatg gtttcctcca ggccgttaac cctggcggta cacaggccat
      781 cggcgtcata gcggtaacgc caggtcacgc cgccccagtc atgctcttcc gtcacccgcc
      841 cacagttgtc gagaagataa cggtatgact caccctgcgg gttgatgact tctgtcagtt
      901 gcagcgtgtc tttatcatag cggtactgcc agcggtggcc ttcaccgtcc gttcgcgcca
      961 ccggcaaatc aaacgggccg tattccattg tccagtccac accgttaccg tccgtccagg
     1021 ccagcaggtt gccgtggcgg tcatagcgga atccttctgc gccggttcct gcccgctcaa
     1081 ggccgctcag tttatggtgc tgctgccact gcaaccgcac tgactgaccg ccggggccgc
     1141 gcatcttcac cggcaggtcg tgaccactgt attcggtgtg gtgttcacgg cccatcacgt
     1201 cggtgagtgt ttccggccgg ttgtggtggt cgtactctat gcccagacgc cgttcatcct
     1261 ggcggaatat ccccgacagc agcccctgag cggtgtacgc cagccgggtc tcttcgccct
     1321 gtggcgatac cagactcacc ggcaggccgc gttcatcgca ctgctgtgtc cacgtctgcc
     1381 cggcagggtc ggtgatgttg ctgagcaggc cgcgctcatc atagcggtac aaccacaccg
     1441 cgccgtctgc cgccgtataa cggcttaact gaccgtgtac ggcgttgtat tcaaatttct
     1501 ccacccgtcc ggcaggggag tggcgggcca gcaggtgatg gaattcatcc cattcatacg
     1561 tggtggtgct gccgtccggc gcttcttccc gcagaatatt gccgtcagga tcatagtaat
     1621 aacggtgaat gcccttgccg tccgtcacgg tggtgcagcc gtcactatag tccagcgtgg
     1681 cgttgtaata gccgtccgcc ccggtgacat tcacgcaccg gccctgttca tcgtaggtga
     1741 aacggctcca cgtctggtcg ttatcggacc agcggatgat ccggtccgca gcgtcgtact
     1801 cataaaacag gtggtagtcc agccgcgcat ccgcttcggt cagccgcccc cgcgcatcct
     1861 gttcgtaggt cgccagcgtc tgtatgccgt tatccgtgcg gtgaatggct ttcaggtagc
     1921 cgctggcgtg gtacgtcagt ttaagccgga tcccgtcggt gtgactgact cccgtgatgc
     1981 ggtactggct gtcgcgctca aacgtgatgc ggttgctggc ggtgtcgctc atcccgccca
     2041 gcatgtacat ccggtcaccg gggctgtgtg ccaggcggtc atcattggta tcgtcagtca
     2101 ccgggtggcg caggcgcatt cccggaaagg cgtcataaaa gaagcggctg ctcagggcat
     2161 cacggtcgaa cagctccagg taatcgtcgc ggcggcgcag gatatactgg gcgtgatacg
     2221 ggttaaccgc cgtatcactg tctccgtcga aggcaaagta cagcgtggct ccctggctga
     2281 tgatatccac ccggtttccc tgttcacgca cccaggcgta ttcgctccag gagtcgctcc
     2341 agcccacgcc cgtcaggcca tgcagtggca gtacagaact gtggaaacgg gtgaaggaga
     2401 gggggagtgt ctggcccagc gtaaagtcgg tacgttcgtc gcagtatgcg ccggttaccg
     2461 gatcgacagg atctgttacc catctgatga ctttattggc aatacttttt aacgacttac
     2521 ctgttttccc cagcaacgcg ccgagctttg cccccagcag cttcccgctt ttccccagca
     2581 ggtttcccag tttgccgagt ttcgccagat tccccagttt tcccagcagt cccgcgggcg
     2641 gcagaagata gctggctatc gttattcccc ccaggatcat tctctgccac ggcgggaact
     2701 ccggctggat atccagatag gtcacctgtt caccgccaat aaagacgtcc ggcgaacctt
     2761 ccacgattgc cgcgctgcac tccagtttgt cgcccttgcg cgcggcaggc tgattgttga
     2821 taaagacgtt gctgctgccc tgggctatcc tcggtggttt gttctccttg tcgcagagcg
     2881 ccgtgtcctc accggcttcc gggaaatcca cgtctttttt gatttcgcca cgcccgccca
     2941 ccatggtggc aaactccatt aataccggac cggcgggaaa cagaagcgat gcgccggcgc
     3001 tgacgcgatc cataataccg gcattgctgt ccgtcagggt gataacagcg ttcccccagg
     3061 ccttcgccag tggcactgct ttttccgctg ccgattcaag caggtcagtg gcaatatccc
     3121 cccatgaagg ttccccctgt ggtggagagg gtattgcttc agtcagggga ggagggggaa
     3181 gggtgacggc ggccctggct gctggcttac cgttaatttt tacgttttca gaaccggtat
     3241 tgatggcgcc gtccgtgtca caaaaactgt ttatccatga ctcaagctgg ttctgtttct
     3301 cctccacata atcactcgcc ttatagctgg catacatcag cgcaggcacc agaaccagcg
     3361 cataagggcc ggtggcgcag gcggccataa tcaggcaacc ttctgcgata gttactatcg
     3421 cccccagcac ggcccctgcc agtgccccca gaaaactttt atgctgaatt tcatcgccca
     3481 gcctggcggc aggtgggccg gacttctttg ctgccagcat ctgtgcaatg gctttgtcgt
     3541 tggctgcccg tttcatctgg ctggcgacca tcgcctgctg tgacagcatt cgcattgcca
     3601 gcgtaccgcc tgcggtcgct ccctgtgggc cgcctggtcc ttcactcatg tgttatccct
     3661 cgtttatgtc agcgcggctg gtccttttgg atgtcattct ccggtggcaa cggggtgaag
     3721 ctctttatca cctccagcag catcgtcttt tgactgttct tcagggataa ggaggtgctg
     3781 aaagtgaagg tcagcagcgt atctccacgc cgctggaata cggctatctg gtcaatccgt
     3841 ccctggttcc cctcatcttc cggaccgttc caggaataat ccagcagcca ggccggggcc
     3901 tctgccagag tgatttcccg gcaggcatgt tcggtgtacc catcaagttt gccctggtac
     3961 agcgccagcg tttcctggta cagctctggt gtcagggttc cctgtggaac ggcttcgcgg
     4021 gtaatgacca ggttgatgcc actgttatct gtggacacca ggacctgcat tgactgatcc
     4081 tgccattgcg atggcagctg aatatgacct tcattaaaag tgaactgcat aaaatactcc
     4141 ctgataatct gaatgaatca gttgatgaaa acagaaccgc ccacgatggt gatgccatcc
     4201 ttcgacagcc tgattaccgt cgattccccg acctgtagct gaatgccatc cggcgtcagc
     4261 gcaattttgc tttcctccac ggccagcata atttgcttaa ttgccgtata agcctgtata
     4321 ccgtcgcctt cgccaccatt gtttacagcg tcaacatgca ctgaaccggc aatcgtgcct
     4381 ctgtcctgaa aaacatcctc agataaagac ccttttagta cgcctgtgat tttgtggcct
     4441 ttttccaccg taagaaggcg gttaccttcc tggatcatga ccgtctggtc attttttacg
     4501 gtttcgcggt gatcgtgttt tacggtggtg gtacggtcgt tgagtacctc ggtatccatg
     4561 ttcttctggg cgtggatgta gacctgttct ttccctgtcg catcgtcaaa cttcagctcg
     4621 ttaaacccgc tgcccatata tgttttggag cggatggtca tctgcgtctt cgttcccggc
     4681 aggctgcccg gcgagcggtt atcttcgtgg taggtgcgcc ccataatgat cggctggtcc
     4741 ggatcgccgt tgaggaagtc cacaatcacc tcctgaccca cgcgcggtat cgccaggttg
     4801 ccaaatcccg gccccgccca cgcctgcgcc acccgtatcc agcacgaact gtcctgattg
     4861 gccgggttat agcggtccca gttgaatttc acccgcaccc ggccatgctc gtcgcagaag
     4921 atttcctctc ccgcaggccc cgtcacgacg gcgctctgcg gaccgtccac tgacggcttt
     4981 ggcttcggct gaggtcgcca cgtccggtct gccggtatta ccgcaaagtg gttattcagc
     5041 gtggtacctg accccctgcg tcccggcacc gcctgcggct gttcgccgtg cagttcgctc
     5101 gccaccacct gccattcccg gttcaggttc gcctgcggat gctccgtcag cacaattcgc
     5161 cgtccaggcc atatctccgg cgagcggctc attccccgcg cggtttcggc gttgtttcgc
     5221 cagccgtcca tctgccagcg ggcaaagttc tgcccgtggg cgctcttgaa acgcccgggc
     5281 cagtcataca cttcatactg tgtgcgctgg taatcctgat gctggccttc ctggtcaaaa
     5341 cgtccggccc agccggggcg tttaaaggtg tagtctttgg tcaccacaga ggaaggacgg
     5401 atttgtgcgc tgtagcggaa ctggctgatg cagagggtgc tcacctcggt acgggtgttc
     5461 gggttccagg ggatttcaaa ggattccggc agatagcgga ccgtgtcgca cagcaccaga
     5521 ctctggtcgg tacttttctg cgcgtgctcc tcatagaaga agatgccttc ctccgctgct
     5581 atccggcaga gaaagtcgta atcggtttca ccgtactgga cacaaaactc acgggaagga
     5641 tgcggctcac tgaacagcgg gctccactcc gtgacgtcat tttcctgcag tatggtgccg
     5701 aggatgcttc tgatgtcctc gttctggaat atacggaagt tctgacgcag tcctgcccgc
     5761 cacagcggcg ggcgcacctt catgctgtac agcatctggt ttttgtcgtt ctccccgagt
     5821 tcaaaccagg tcaccacgcc tttcacccgg cgctgtacgt catcgccctg ccataccgtc
     5881 aggtaggcca ttttgtccag cacctgcgca aattcaaggg agagaaactg ctgactgacc
     5941 agagaaaggt caagggaaaa aagtgaagag agtgactggg tgagatgaaa ggagaccacc
     6001 gcaaaagcgt ccggtggcag gccgtccact tccagtgtga aacgtaatcc ggttgacata
     6061 gcaacctccg tgaaaatgaa taataatccg catatattgc atgtacaaca tgtcgggtaa
     6121 tgagataagc gggccggctt caggcgttac cggggaatat cagaaaagtg tcatggttat
     6181 gtgatgattt tgtccgggat tttacttcag agaaacgggg ggctgttgac tcaacaaaat
     6241 aatcacaaac tcaactgacg ggttcacgat gttattaagg acaggctcgt atattacatc
     6301 gagtaacaaa aaaacacctg atatgcaaag tcaaaccaat atttgtaatg tctttttatt
     6361 cacaataata aataactcaa taaacattat tgaaatgaat gatggtttgt ataaggaata
     6421 tacgctaccg ccccaccctg ataacatgat ctgcacattg ctatcttaac gtcgttagac
     6481 gctggcttaa tagtgaata
//
LOCUS       DAZFLD010000225         6485 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.226, whole genome shotgun sequence.
ACCESSION   DAZFLD010000225 DAZFLD010000000
VERSION     DAZFLD010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6485
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.226"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            126..776
                     /gene="csgD"
                     /locus_tag="ACHKBL_004170"
     CDS             126..776
                     /gene="csgD"
                     /locus_tag="ACHKBL_004170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm master transcriptional regulator CsgD"
                     /protein_id="HFO5820959.1"
                     /translation="MFNEVHSIHGHTLLLITKPSLQATALLQHLKQSLAITGKLHNIQ
                     RSLDDISSGSIILLDMMEADKKLIHYWQDTLSRKNNNIKILLLNTPEDYPYRDIENWP
                     HINGVFYAMEDQERVVNGLQGVLRGECYFTQKLASYLITHSGNYRYNSTESALLTHRE
                     KEILNKLRIGASNNEIARSLFISENTVKTHLYNLFKKIAVKNRTQAVSWANDNLRR"
     gene            781..1170
                     /gene="csgE"
                     /locus_tag="ACHKBL_004171"
     CDS             781..1170
                     /gene="csgE"
                     /locus_tag="ACHKBL_004171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgE"
                     /protein_id="HFO5820960.1"
                     /translation="MKRYLRWIVAAEFLFAAGNLHAVEVEVPGLLTDHTVSSIGHDFY
                     RAFSDKWESDYTGNLTINERPSARWGSWITITVNQDVIFQTFLFPLKRDFEKTVVFAL
                     IQTEEALNRRQINQALLSTGDLAHDEF"
     gene            1195..1611
                     /gene="csgF"
                     /locus_tag="ACHKBL_004172"
     CDS             1195..1611
                     /gene="csgF"
                     /locus_tag="ACHKBL_004172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgF"
                     /protein_id="HFO5820961.1"
                     /translation="MRVKHAVVLLMLISPLSWAGTMTFQFRNPNFGGNPNNGAFLLNS
                     AQAQNSYKDPSYNDDFGIETPSALDNFTQAIQSQILGGLLSNINTGKPGRMVTNDYIV
                     DIANRDGQLQLNVTDRKTGQTSTIQVSGLQNNSTDF"
     gene            1638..2471
                     /gene="csgG"
                     /locus_tag="ACHKBL_004173"
     CDS             1638..2471
                     /gene="csgG"
                     /locus_tag="ACHKBL_004173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgG"
                     /protein_id="HFO5820962.1"
                     /translation="MQRLFLLVAVMLLSGCLTAPPKEAARPTLMPRAQSYKDLTHLPA
                     PTGKIFVSVYNIQDETGQFKPYPASNFSTAVPQSATAMLVTALKDSRWFIPLERQGLQ
                     NLLNERKIIRAAQENGTVAINNRIPLQSLTAANIMVEGSIIGYESNVKSGGVGARYFG
                     IGADTQYQLDQIAVNLRVVNVSTGEILSSVNTSKTILSYEVQAGVFRFIDYQRLLEGE
                     VGYTSNEPVMLCLMSAIETGVIFLINDGIDRGLWDLQNKAERQNDILVKYRHMSVPPE
                     S"
     gene            complement(2535..3026)
                     /gene="ycdZ"
                     /locus_tag="ACHKBL_004174"
     CDS             complement(2535..3026)
                     /gene="ycdZ"
                     /locus_tag="ACHKBL_004174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HFO5820963.1"
                     /translation="MNILLSIAITTGILSGIWGWVAVSLGLLSWAGFLGCTAYFACPQ
                     GGLKGLAISAATLLSGVVWAMVIIYGSALAPHLEILGYAITGIVAFLMCIQAKQLLLS
                     FVPGTFIGACATFAGQGDWKLVLPSLALGLVFGYAMKNSGLWLAARSAKTAHREQQIK
                     NKA"
     gene            complement(3128..3682)
                     /gene="ycdY"
                     /locus_tag="ACHKBL_004175"
     CDS             complement(3128..3682)
                     /gene="ycdY"
                     /locus_tag="ACHKBL_004175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone YcdY"
                     /protein_id="HFO5820964.1"
                     /translation="MNEFSILCRVLGSLYYRQPQDPLLVPLFTLIREGKLAANWPLEQ
                     DELLTRLQKSCDMTQVSADYNALFIGDECAVPPYRSAWVEGATEAEVRAFLSERGMPL
                     ADTPADHIGTLLLAASWLEDQSTEDESEALETLFSEYLLPWCGAFLGKVEAHATTPFW
                     RTMAPLTRDAISAMWDELEEDSEE"
     gene            complement(3706..4443)
                     /gene="ycdX"
                     /locus_tag="ACHKBL_004176"
     CDS             complement(3706..4443)
                     /gene="ycdX"
                     /locus_tag="ACHKBL_004176"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding phosphatase"
                     /protein_id="HFO5820965.1"
                     /translation="MYPVDLHMHTVASTHAYSTLSDYIAQAKQKGIKLFAITDHGPDM
                     EDAPHHWHFINMRIWPRVVDGVGILRGIEANIKNVDGEIDCSGKMFDSLDLIIAGFHE
                     PVFAPHDKATNTQAMIATIASGNVHIISHPGNPKYEIDVKAVAEAAAKHQVALEINNS
                     SFLHSRKGSEDNCRAVAAAVRDAGGWVALGSDSHTAFTMGEFEECLKILDAVDFPPER
                     ILNVSPRRLLNFLESRGMAPIAEFADL"
     gene            complement(4498..5435)
                     /gene="ghrA"
                     /locus_tag="ACHKBL_004177"
                     /pseudo
     CDS             complement(4498..5435)
                     /gene="ghrA"
                     /locus_tag="ACHKBL_004177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415551.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016618 - hydroxypyruvate reductase
                     [NAD(P)H] activity [Evidence IEA];
                     GO_function: GO:0030267 - glyoxylate reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrA"
     gene            5668..5755
                     /locus_tag="ACHKBL_004178"
     tRNA            5668..5755
                     /locus_tag="ACHKBL_004178"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5702..5704,aa:Ser,seq:gga)
     gene            complement(5905..>6485)
                     /locus_tag="ACHKBL_004179"
     CDS             complement(5905..>6485)
                     /locus_tag="ACHKBL_004179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HFO5820966.1"
                     /translation="NLDREIWRDLMQCSGMLSLMDAQARDTWYRSLEYDNFPEISEEN
                     ILSTFEQLHQNKDEVFERGVINVFRGLSWNYKTNCPCKFGNKIIVNNLVRWDQWGLHL
                     ITGQQADRLADLERMLHLFSGKPIPDNRENITIHLDDHIRSLQGKECYEDEMFIIKYF
                     KKGSAHITFRKPELVDRLNDIIAKHYPDMLAV"
BASE COUNT         1732 a         1605 c         1480 g         1668 t
ORIGIN      
        1 attttaccca tttagggctg atttattact acacacagca gtgcaacatc tgtcagtact
       61 tctggtgctt ctattttaga ggcagctgtc aggtgtgcga tcaataaaaa aagcggggtt
      121 tcatcatgtt taatgaagtc catagtattc atggtcatac attattgttg atcactaaac
      181 cttctttgca ggcgacagct ctcttgcagc accttaagca atcgctggca attacaggaa
      241 aattacataa tattcaacgt tctctggacg atatctcttc aggctctatt attcttctgg
      301 atatgatgga agcggataaa aagcttatcc attattggca ggatactttg agcaggaaaa
      361 acaacaatat caaaatattg ttgctaaata cgcctgaaga ttacccgtac cgcgacattg
      421 aaaactggcc tcatatcaac ggcgtttttt atgccatgga ggatcaagaa cgtgttgtca
      481 atgggttgca aggcgtcctg cgcggcgaat gctactttac gcaaaagctt gccagttacc
      541 tgattacgca ttcaggtaac tatcgttata acagcacgga atcagccctc cttactcatc
      601 gggaaaaaga gatcctgaat aagctgcgta tcggcgcgtc taataacgag atcgctcgtt
      661 cgttgttcat cagcgaaaat acggttaaaa cgcatcttta taatcttttc aagaagatag
      721 ccgtaaaaaa ccggacacaa gcggtttcct gggcaaacga taacctcagg cgataaagcc
      781 atgaaacgtt atttacgctg gattgtggcg gcagaatttc tgttcgccgc agggaatctt
      841 cacgccgttg aggtagaagt cccgggattg ttaactgacc atactgtttc atctattggc
      901 catgattttt accgagcctt tagtgataaa tgggaaagtg actatacggg taacttaacg
      961 attaatgaaa ggcccagtgc acgatgggga agctggatca caataacggt gaatcaggac
     1021 gttattttcc agactttttt atttccgttg aaaagagact tcgagaaaac tgtcgtcttt
     1081 gcactgattc aaactgaaga agcactaaat cgtcgccaga taaaccaagc gttattaagt
     1141 acgggcgatt tggcgcatga tgaattttaa ataataaatt gttcggaggc tgcaatgcgt
     1201 gtcaaacatg cagtagttct actcatgctt atttcgccat taagttgggc tggaaccatg
     1261 actttccagt tccgtaatcc aaactttggt ggtaacccaa ataatggcgc ttttttgtta
     1321 aatagcgctc aggcccaaaa ctcttataaa gatccgagct ataacgatga ctttggtatt
     1381 gaaacgccct cggcgttaga taactttact caggccatcc agtcacaaat tttgggtggg
     1441 ctactgtcga atattaatac cggtaaaccg ggccgcatgg tgaccaacga ttatattgtt
     1501 gatattgcta accgcgatgg tcaattgcag ttgaacgtga cagatcgtaa aaccggacaa
     1561 acctcgacca tccaggtttc gggtttacaa aataactcaa ccgattttta agccccagct
     1621 tcataaggaa aataatcatg cagcgcttat ttcttttggt tgccgtcatg ttactgagcg
     1681 gatgcttaac cgccccgcct aaagaagccg ccagaccgac attaatgcct cgtgctcaga
     1741 gctacaaaga tttgacccat ctgccagcgc cgacgggtaa gatctttgtt tcggtataca
     1801 acattcagga cgaaaccggg caatttaaac cctacccggc aagtaacttc tccactgctg
     1861 ttccgcaaag cgccacggca atgctggtca cggcattgaa agattctcgc tggtttatac
     1921 cgctggagcg ccaaggctta caaaacctgc ttaacgagcg caagattatt cgtgcggcac
     1981 aagaaaacgg cacggttgcc attaataacc gaatcccgct gcaatcttta acggcggcaa
     2041 atatcatggt tgaaggttca attatcggtt atgaaagcaa cgttaaatcg ggcggggttg
     2101 gggcaagata ttttgggatc ggtgcagaca cgcaatacca gcttgatcag attgccgtga
     2161 acctgcgcgt cgtcaatgtg agtaccggcg agatcctctc ctcggtgaac accagtaaga
     2221 cgatactttc ctatgaagtt caggcggggg tcttccgctt tattgactac cagcgcttgc
     2281 tggaagggga agtaggttac acctcgaacg aacccgttat gctgtgcctg atgtcggcta
     2341 tcgaaacagg cgtcattttc ctgattaatg atggtatcga ccgcggtctt tgggatttgc
     2401 aaaataaagc agaacggcag aatgacattc tagtgaaata ccgccatatg tcggttccac
     2461 cggaatcctg atagataaaa atgccggaat tatcatccga cattttacgg tttaagcgaa
     2521 agcgtgagtg cccctcacgc tttatttttg atttgctgct cacggtgagc cgtctttgca
     2581 ctacgcgccg ccaaccacag gccgctgttt ttcattgcgt aaccaaatac cagtccaagc
     2641 gccagcgaag gtaacaccag tttccagtca ccctgcccgg caaacgtcgc gcatgcgcct
     2701 ataaacgtcc ccggaacaaa tgaaagcagc agctgtttgg cctggataca catcagaaac
     2761 gcgacaatac cggttatggc ataaccgagt atttccagat gtggtgccag tgcactaccg
     2821 taaataatga ccatcgccca caccacgcca ctaagcaggg ttgcagcgga gatcgccagc
     2881 cctttcagcc caccttgtgg gcaggcaaag taggccgtac agcccaggaa gcccgcccag
     2941 cttagtaagc ccagggaaac agccacccat ccccagatac cggagagaat gcctgttgtg
     3001 attgcaatag agagaagtat gttcatgccg cgcatgatag cagaaaacgc ggccatgaat
     3061 gagatcgagc acacatttta tgcaacaaca aattacccgt tgcagttatg agtgatgcag
     3121 atcacattta ctcttcagaa tcttcttcca gctcgtccca cattgcacta atagcatcgc
     3181 gggttagcgg tgccatggtg cgccagaaag gcgtagttgc atgggcctcc actttgccaa
     3241 ggaacgcacc acaccagggt aacagatact cactgaacag tgtttccagt gcttcgctct
     3301 catcttccgt tgactgatct tccagccagg aagccgcaag cagcaatgtg ccgatgtgat
     3361 cggctggcgt atccgctagt ggcatccctc gttcggaaag aaaagcgcgc acttccgctt
     3421 ccgttgcgcc ctcaacccat gcgctacgat atggcggcac agcacattca tcgccgataa
     3481 acaacgcatt gtaatcggca gagacttgcg tcatatcaca gcttttctgt aaacgcgtca
     3541 gtaactcatc ctgctccagt ggccagttcg cagccagttt cccctcacga atcagggtaa
     3601 acaacggcac cagtaaagga tcttgcggtt ggcggtaata gagcgaaccc agcacacgac
     3661 agaggataga aaactcattc attaatatat ttccattact agtaattaaa ggtctgcaaa
     3721 ttccgcaatc ggtgccatac cgcgagattc aaggaagtta agtaagcggc gcggagaaac
     3781 attcaaaatg cgctctggcg gaaaatctac cgcgtcgagg attttaagac actcttcaaa
     3841 ttcccccatg gtaaacgcag tgtgagaatc cgagcctaat gccacccaac ctccagcgtc
     3901 acgtaccgct gcggctaccg cccgacagtt gtcttcactg cccttacgtg agtgtaaaaa
     3961 tgaggaatta ttgatttcca gcgccacctg atgtttcgcg gctgcttcag caacggcttt
     4021 aacatctatt tcatatttcg gatttccggg atggcttatt atatgcacat tgccgctggc
     4081 gatagtggcg atcatcgctt gcgtattggt cgctttgtca tgcggcgcaa acaccggctc
     4141 atgaaaaccg gcaataatca gatccagcga atcaaacatt ttgccactgc agtcaatttc
     4201 accgtcaaca tttttaatgt tagcttcgat gccgcgcagg atcccaaccc catcaaccac
     4261 tcgcggccag atacgcatgt taatgaagtg ccagtgatgc ggcgcatctt ccatatccgg
     4321 gccatgatcg gtgatcgcaa aaagtttaat gcccttttgt ttggcctggg caatgtaatc
     4381 actcaatgtg ctatatgcat gcgtgctggc aacggtatgc atatgaaggt cgacgggata
     4441 cataactctc tcctgtattc aattcccgcc aaggatagca ggaaacctga tgctttatta
     4501 gtagccgcgt gcgcggtcga cttgcccgca gaccctctcc cctttttcga gctgggcaat
     4561 ggtgcgcgaa atgtaatcca cagcttcagc gggacgggta atcgcggcga catgtggtgt
     4621 tatcgtcacg cgtggatgtt gccagagcgg actttccggc ggtaagggtt cacgattaaa
     4681 aacatccagc atgcgccttt aactttgccg ctatccagcg ccgcgagcag gtcatcttcc
     4741 acgacatgaa caccacgcgc caggttgagg agatacgcgc catccggtaa tttttcgagt
     4801 aattgttgat taataatgcc gacggtttca ggtgtattcg gtaacaaatt aatcaatacc
     4861 cgacattggc tcagaaatgc agagagttct tcccgtccgg caaagctttg cacgccaggc
     4921 cacgatttac gggttcgact ccagcaacgc agcgggaagc gccaggtttg cagactctgt
     4981 gcaactttac tgcccagtac acctgcaccc aaaatgccga tggtaaaatc ttcccgatga
     5041 tattcaggca gcggttgcca atgcgaacta ttttgctgga tgcggtaatc gtcaaaacgt
     5101 cgaaaccaat gcagcacctg actgacagca tattcctgca tttgctcgcc cataccggta
     5161 tcttccaggc gaaaaagtgg aacagaaggc ttcagcattt cagggtgagc ctgtagcttg
     5221 ctcaaaatag aatcaacacc tgccccaagt gcaaacaccg ctttaagatc gcgcccagcc
     5281 agcatttcaa caggaggatg ccagactaaa gcataatcgg ctgattcatt gtctccgctt
     5341 ttccatgctc tgactcttgc ctgaggaata gctttgtgca gtgcctcaat ccaccattgg
     5401 gtatcgaacg ttgggtgata aaagatgata tccatactga ctcccgaaga gtgttggcga
     5461 aatttattcg cacttatcgt tatgatctac aaagaccagc agcataacaa atccgtggtc
     5521 ggtggcaaaa aaagcaaatt tcgcttatta aaactgcata ttgattgaag tttgaataat
     5581 cgagcgattt tttaaaaaag tttgttgacc tcaggtcatg atttccctaa attagcgccc
     5641 gttccagcaa gacaggaacg acaatttggt gaggtgtccg agtggctgaa ggagcacgcc
     5701 tggaaagtgt gtatacggca acgtatcggg ggttcgaatc cccccctcac cgccagatta
     5761 caagcgcatg ttattgttta acatgcgctt ttttatttat ccttccagac agtatgccac
     5821 cgggtatgcc atctgaaacc ggtttatatg ccccacgatc atatcttatc ggacaatttc
     5881 cgatctttga gcatactggc aaagctatac tgccagcatg tcaggatagt gttttgcgat
     5941 aatatcattc agtctgtcaa ccagttctgg cttcctgaac gtgatgtgcg cagagccctt
     6001 cttaaagtat ttaataatga acatctcatc ttcatagcat tcttttccct gaagagaccg
     6061 gatgtgatcg tccagatgaa tagttatgtt ttccctgttg tcggggatcg gtttgccgct
     6121 gaacagatgc agcattcttt ccaggtcggc aagtctatct gcctgttgcc cggtgataag
     6181 atgcaatccc cactggtccc acctcaccag attgttgacg ataattttat tgccaaattt
     6241 acaggggcaa ttggttttgt aattccagct caaccctctg aagacgttga tcactcctcg
     6301 ctcaaacact tcatccttat tctggtgtag ctgctcaaac gtactcagaa tattctcttc
     6361 actgatttcc ggaaaattat cgtactccag tgaccggtac catgtatcac gggcctgtgc
     6421 atccattaag gaaagcatcc cggagcattg catcaggtca cgccagattt ctcgatcgag
     6481 attgc
//
LOCUS       DAZFLD010000226         6479 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.227, whole genome shotgun sequence.
ACCESSION   DAZFLD010000226 DAZFLD010000000
VERSION     DAZFLD010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6479
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.227"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..239)
                     /locus_tag="ACHKBL_004180"
     CDS             complement(<1..239)
                     /locus_tag="ACHKBL_004180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFO5820967.1"
                     /translation="MKILPVISTKGGEGKSTQSANLAGFLADAGLRTLLIDGDHAQPT
                     ASNIFPLTYEAPAGLFELLMRTADLSHPDNIISRTA"
     gene            555..1739
                     /locus_tag="ACHKBL_004181"
     CDS             555..1739
                     /locus_tag="ACHKBL_004181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO5820968.1"
                     /translation="MALTARQVETARPKEKDYKLSDERGLYLLVKTTGARYWRLKYRI
                     AGKEKKLALGVYPDVSLAEARIKRDDARKIISEGGDPGEKKRKEKLTQKISATNTFHA
                     LATEWHQHKSLSWSESYARSVLEALDKDIFPYLGKRSVTDILPLEMLEILRRIEKRGS
                     LEKLRKVRQYCNQIFRYAIATGRATVNPASELTSTLAAPKAAHFPHLRADELPVFLRK
                     LAEYHGSPVTRMATNLLLLTGLRTIELRSAEWSEIDFDNALWTIPESRMKMRRKHVVP
                     LSRQVTDILQQLKTFSGQYRLVFPGRCDINKPMSEASINMVLKRIGYDGRATGHGFRH
                     TMSTILHEQGFNSAWIEMQLAHVDKNSIRGTYNHALYIDGRREMMQWYADYIDSLSEQ
                     VS"
     gene            2068..2649
                     /locus_tag="ACHKBL_004182"
                     /pseudo
     CDS             2068..2649
                     /locus_tag="ACHKBL_004182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709438.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
     gene            2736..3014
                     /locus_tag="ACHKBL_004183"
                     /pseudo
     CDS             2736..3014
                     /locus_tag="ACHKBL_004183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(3282..4286)
                     /locus_tag="ACHKBL_004184"
     CDS             complement(3282..4286)
                     /locus_tag="ACHKBL_004184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013315785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFO5820969.1"
                     /translation="MQNITLIGIDLGKHSFHIHCQDKSGKVLLRKKFTRTKLIEFLAG
                     CASSTVVMEACAGAHFMARRIADLGHEAKLISPQFVRPFVKSNKNDFVDAEAICEAAS
                     RPSMRFVQPRTEAQQAMRALHRVRESLVSDKVKTTNQMHAFLLEFGISMPTGVAVVKR
                     LSTVLAENELPPYLAQLLMRLHAHYIYLVGQITELEIALEQELSRDEVGQRLQTIPGI
                     GPITASVLSSQLGDGKQYSCSRDFAASTGLVPRQYSTGGKNTLLGISKRGDKNLRRLL
                     VQCARSFIQRIENQSGRLADWVRALLARKHSSVVCCALANKLARIAWAITTQQTVFER
                     "
     gene            4335..5290
                     /locus_tag="ACHKBL_004185"
                     /pseudo
     CDS             4335..5290
                     /locus_tag="ACHKBL_004185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            5330..5809
                     /locus_tag="ACHKBL_004186"
                     /pseudo
     CDS             5330..5809
                     /locus_tag="ACHKBL_004186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709438.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
     gene            5952..6181
                     /locus_tag="ACHKBL_004187"
                     /pseudo
     CDS             5952..6181
                     /locus_tag="ACHKBL_004187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001327330.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6168..>6479)
                     /locus_tag="ACHKBL_004188"
     CDS             complement(6168..>6479)
                     /locus_tag="ACHKBL_004188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001608786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HFO5820970.1"
                     /translation="HGLSVSDSANPVHHDGHVQGGYSVPLIITASDITSHQSVSRKIS
                     ARHFAGIFQWLTGIRTENIPPFNPLTDEDNEPVMVFNGERNVPADSLKPQPLILPVRG
                     K"
BASE COUNT         1785 a         1537 c         1524 g         1633 t
ORIGIN      
        1 gccgtacggg aaatgatgtt gtcaggatga ctgaggtcag ccgtgcgcat cagtaattca
       61 aaaagaccgg ccggcgcttc atacgtcagc ggaaagatat tgcttgccgt gggctgtgca
      121 tggtctccgt ctatcagcag agtacggagg ccggcatctg ccagaaaacc ggcaagatta
      181 gcagactggg ttgattttcc ttcgccacct ttagtggata taaccggtaa aattttcata
      241 tagcacactc tttaaagtat gcaaccaatc acttacacta cggaaaccag ccagaatatt
      301 cacttcacta cctgaatact cgcaacacgg ggattgaaaa tccccgtgtc cttggttcga
      361 ttccgagtcc gggcaccact ttcacttcca gattcatctc ccgaactcca cgaaactcaa
      421 gtataatcac atcgttacaa gcaatctgaa catcctgaac tccaccgaac tctactgaac
      481 tctttctcaa tcaagctttc attgggggca ccggtggggg ctcattatgt tcagtcagtt
      541 tcaggagccc ccatatggca cttacagcca gacaggtcga aacagccaga cctaaagaaa
      601 aagactataa actctctgac gaacgtggtt tatatctgct ggtaaaaacc acgggagccc
      661 gctactggcg gcttaaatac cggatagcag gaaaagagaa aaaactggcc ctcggcgtct
      721 atcccgacgt ctcccttgct gaggccagaa tcaaacgtga cgatgcccga aaaatcatct
      781 ccgaaggtgg tgacccgggc gaaaagaagc gaaaggaaaa actcactcag aaaatctctg
      841 ccaccaatac gttccatgcc ctcgctacgg aatggcacca gcataaatct ttgtcatggt
      901 ctgaaagtta cgccagaagc gtactggaag cgctggataa agatattttc ccgtatctgg
      961 gcaaacgaag cgttacggat atcctcccgc tggaaatgct ggaaattctg cgccgcatag
     1021 aaaaacgtgg ctcgctggaa aaactgcgta aggtacgtca atactgtaat cagatttttc
     1081 gttatgccat cgccaccgga cgagccactg tcaatccggc atctgaactg accagtacgc
     1141 tggcggcgcc aaaagctgca cacttccccc acctgagagc agatgagctc cctgtttttc
     1201 tcaggaagct cgctgaatat catggcagtc ctgttacccg catggcgaca aatctgctgc
     1261 ttctgacagg cctcagaacg attgagctac ggtccgctga atggtcagaa attgattttg
     1321 ataatgccct gtggacaatc cctgaaagcc gcatgaaaat gcgacgtaaa catgtcgtac
     1381 cactgtcacg acaggtcact gacattctgc aacaactcaa aactttctcc ggacaatacc
     1441 ggctggtttt cccgggacgt tgtgatatca acaagccaat gagcgaagcc agcatcaata
     1501 tggtgctcaa acgtatcggt tacgatggca gagcaaccgg tcatggtttt cgtcacacca
     1561 tgagtaccat tctgcacgaa caggggttta attctgcctg gattgaaatg cagttagctc
     1621 atgtggataa aaattccatc aggggtacct ataatcatgc cctgtatatt gatggtcgcc
     1681 gtgaaatgat gcagtggtat gctgactaca ttgattcgct ttctgagcag gtaagttaag
     1741 ctgagaacac gttattctga ttttcactca cttcacgatt attcctccgg cctgcaggtt
     1801 cggaggattc ttatttttat gttttatttt cagataaagt ttttgacaaa acgtttattc
     1861 tgcatcagca aaattatcac aaaacaacgc aatcacagac aaattaacat ttttcagacg
     1921 ggtatattga gtgcccacaa tgtaagacag gcaggcatcc ggtgacatgg taaaatatta
     1981 atccagggca aaaacaaggc ttgatacaca acatcatgac ttttatgctc tcagactatg
     2041 ccaccacaga ttttacaggc tcagaaaatg aatgacagat tacgctttga agtgaacgat
     2101 aaccagggct gtttcgtgtt cccggacgcc tggtttggct cgttgctgga tgagtttgac
     2161 gaactaattg atgcatacga cagagatgaa atcagcgaaa caagttatat caacaaactc
     2221 agacgccttg cccgacagga gcctgacttt attgatgtac acgcttacct ggcatgcgca
     2281 tttctggagc agaatgcccc gcgtaaggca ctgaacgctg ctcttaaagg actggcagca
     2341 ggtaatcgtc tcatcccgga aagtttcagt ggtgaaatca tctggatgca tcccgaaaac
     2401 cggccttatc tgcgcgcgct ttatgcagca attctcgcaa atgtgcactt acagcgtcat
     2461 caggatgcag ctctgctcac tgataaaata ctggcataca atcctgaaga caatcaggga
     2521 gcacgctggc tactgggacc ggaactgtta cgtactggcg accatgaaca ggcacgccat
     2581 gtactgaaag cgcatgcaga tgaattttca ccttactggt atgaactcgg ccttctgcat
     2641 tttctgaacc ggccccttga aacgccagac agtggatgta tcttaaaata agagataggc
     2701 ttggatatat gtctaacact agttctaatt ttgagatgac tggaattttg ctggggcagg
     2761 agcttcgtaa acgtaaaact cctcaggaaa aaatcgccat tattcagcag acaatggaac
     2821 ccggtatgac ggtgtcccat gtggcgcgtt tgcatggtat tcagcccagc ctgttgttta
     2881 agtggaaaaa acaatatctg gaaggaagtc tcactgctgt tgctgccgga gaggatgtcg
     2941 ttcctgcctc agaactggct gccgctataa aacaaataaa agagttacag cgcttgctgg
     3001 gtaagaaatc tatggtcccc accatttttg caacactgat tttgattgat gttggcttgc
     3061 ctaaatctat ccggcgtcat tatgggcatg gatgcccgcg cctcgatgag ttccgcacct
     3121 gatgagccca aaaaaccatc cggcttcagt gagccatttt cttgctgagg cttttcatca
     3181 ggccgatatg ccgttcatgt catcaataat cagtcttcgc aaaaccagat tgggaactac
     3241 tttaattcat gattaacctg ccagccagac tggcgccatc gttacctttc gaacacagtc
     3301 tgctgtgttg tgattgccca cgctatccgg gctaatttgt tggccagtgc gcaacatact
     3361 acgctggagt gttttcttgc caacaacgcc ctgacccaat ccgccaacct tcctgactga
     3421 ttctcgattc tctgaataaa ggatcttgcg cactgaacga gtaaccgtcg cagattttta
     3481 tctccacgtt tacttatccc taaaagtgta tttttaccac ctgtgctgta ctgccggggt
     3541 accagaccgg ttgaagcagc aaagtcccga ctgcagctat attgtttacc gtctcccagc
     3601 tgcgatgaca gtacgctggc ggttatcgga ccaataccgg gtattgtctg aagacgttgt
     3661 cctacctcat cacgactcag ttcctgctcc agtgcgattt caagctcggt gatctgtccg
     3721 acaagataaa tataatgggc atgtaagcgc atcagtaact gcgccagata gggaggaagt
     3781 tcgttctctg caagtactgt tgaaagtcgt tttaccactg cgactcctgt cggcatactg
     3841 atgccaaatt ccagcaaaaa agcatgcatc tgattggtcg ttttgacttt atcactgacc
     3901 aatgattccc tgacccggtg aagcgcccgc atggcctgct gagcttctgt tcgtggttgc
     3961 acaaatcgca tggagggacg agatgccgct tcgcatatgg cttctgcatc gacaaaatcg
     4021 ttcttgttgc tcttaacaaa agggcgaacg aactgcggag aaatcaattt tgcttcatga
     4081 cctaaatcag caatccggcg agccataaaa tgagctccgg cgcaggcttc catcacaacg
     4141 gtagacgagg cacagcctgc cagaaactca ataagtttgg tacgggtaaa ctttttacgc
     4201 agcaatacct tgcctgactt atcttggcag tgaatgtgaa aggaatgctt gccaagatct
     4261 ataccaataa gcgtaatgtt ttgcatgatg gtcttctcca tgaaagaaaa acactctact
     4321 agggtacagc tcacagggtg gtggggacca tctcattaac catggaagtt gagatcctca
     4381 aagaagcggt ggagtatggt cagtcgcgaa aatggatagc gcatgcgccc ttgttgccaa
     4441 cggacaagga ataaccctgg taagccgtat tctgggcgtg tcgcgtgcgc aactggcaca
     4501 acggatgaaa acatctggta ataagccgaa taaacgtcgt cgcagatgtg atgaggctgc
     4561 ggatgcggaa gtgttagcac gtatccttga tatcatcagc gatatgccga catatggcta
     4621 tcgtcgtgtc tgggccattc tcagacgaca gtcccgtaac aaaggagtgc cgttcgtgaa
     4681 tgcaaagcgc gtttatcgga taatgagcga aaataacctg ttgttacatg acaaaccttc
     4741 acggccgcag cgggaacata aaggccgaat atcggtgaag gaaagcgacc agcgctggtg
     4801 ttccgatggc tttgagttcg gttgtgatga tggcgcaaaa ctgcgtgtca cgttcgcact
     4861 ggactgttgc gatcgtgaag ccatagactg ggccgcaagc acgggaggtt atgacaaagc
     4921 aacggtgcag gatgtcatgt taggagcaat agaaaaacgc tttggcgata aaggagcgga
     4981 acggccaatc cagtggctga ctgataacgg ttcggcttat acagctcatg aaacacggca
     5041 gttcgccaga gagttgaatc tggagccctg catgacggca gtcagtagcc ctcagagcaa
     5101 tggaatggca gaaaggttcg tgaaaacgat gaaagaagac tacatcgcgt tcatgccgaa
     5161 accgaatgta agaacggcat tgcataacct tgcagtggcg atcgagcatt acaatgaaac
     5221 ccatccgcat agtgcgttag gttatctctc accaagggaa tatcgacgtc agcgggtaac
     5281 gtcaacttaa gatacaacga ctgtctgaat atcgtgggtc aaggacagcc ttctgcattt
     5341 tcttaatggg gaactggtaa aagctgcaac tgcatttcgt cgcggctttg cggcaaatac
     5401 ttacatcgca gaaatactgt gcggaaatct gcatccattt ccacttgctg tatggcataa
     5461 cttttcaggc ggtccggata ctgctgaaga ttattatgcc acataccacc cgttatgggg
     5521 gcaatatccg gaagcattac tgtttgtaaa ctggctctat aatcattcct ctgtgctgca
     5581 tgaacgggca gaaatcatta aatgcgccga aatgctgatg caggaagatg atttcgaaat
     5641 atgcgaaagt attttaagac agcaggagaa gttgcgtgaa agaattgatg agacgctttc
     5701 tgagaaaatt gtacagaaat gcagaaatat gaatggtgaa tatgtctggc cctggatatt
     5761 gccgttttca gcggcaggca tgaaacatac tggcatacag tatcagtaga tattgcatta
     5821 gtgtatcctg cacacaagta ataatttatc caccaataat aacactgtta atgtcccctt
     5881 cccctggttg tcagccaggg gttatcttct gaatatttct tttgaaaagg ataacacagt
     5941 aaattatttt tatgaattat cccatggact cattaacacc ctttcataat gctttattgt
     6001 caaacacgtt atggctgaca tcaaaaaaac cggatttcct ctgccagcgg gtaatcacct
     6061 ccccggtgtt ttcggttggt ctggttactc cagtctggta attagcaaga taatcgatat
     6121 aaacagtgga aaactcatcg tacataatct ggtgatgaac attacgctta ttttcccctg
     6181 accggaagaa tcagaggctg cggtttcaga ctgtctgccg gtacattcct ctccccgtta
     6241 aaaaccataa cgggttcatt atcttcgtct gtcagcggat tgaatggcgg tatattttca
     6301 gtacgaatgc cggtcagcca ctgaaaaata cctgcgaaat gacgggcact gattttcctg
     6361 ctgacggact gatgagacgt gatgtcactg gcggtaataa tcaggggaac gctgtagcct
     6421 ccctgcacat gaccatcatg atgaacagga ttggcactat cgctgaccga cagcccatg
//
LOCUS       DAZFLD010000227         6439 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.228, whole genome shotgun sequence.
ACCESSION   DAZFLD010000227 DAZFLD010000000
VERSION     DAZFLD010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6439
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.228"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..2749
                     /locus_tag="ACHKBL_004189"
     CDS             <1..2749
                     /locus_tag="ACHKBL_004189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFO5820971.1"
                     /translation="GLAGDALHKIGKGTLVVAGSGENPGTLNTGDGTVILAQKADAAG
                     RVRAFSEVSIVSGRPVVVLQDSHQIEGDRIRWGYRGGTLDINGNDIAFNRLAAADEGA
                     VLTSRARPATVRLDFSQSGQKAVMWHGHFTGNLSVLNNTSSAVDFIMDGGADISGSFT
                     QQGGGLYIQGHPVVHAVSSEAVATALRKQGDNSVLTQPVSFAQKDWESRTFSIGQLKL
                     KGAAFSLSRNATLTGDIDADNATMVLGSDSLYLDMKDGTGSSSAPVKGTSAVGGASGS
                     TTFRGNVNMRHSALTVRDHFTGSITASDSRIAVSSENVRLEGNSRLTSSALTVSDGGR
                     LHVKGGLETDGGVTLDRGTLLVDGGSVRNDVYERLLAWSEERGGLNGSGEYDFMTGAA
                     GLLRSYVRGSAGNVNLQNAAWMMTGNSSVKHLESSGSALYFSRPGGEFHTLTAGSMDI
                     SDSVLVMRTDLNHSDQLRVTESLRGKNNLLLVDFTERSDGQKALNIPLVTAPAGTGAD
                     VFSVKTRDTGFSHITPVVRAEQGTGGTAWQLNVVQPETAAEPVATRQDAETPNPVEAV
                     SPLPSPVSAPSSAPEASVTDVLTGENAGESGEYRDETRWLLTGYRSTVNSSAVRDAGM
                     LMSMGHRNFINEVNNLNKRMGDLRDINGEAGAWARIMSGTGSAGGGFSDNYTHVQVGA
                     DKKHELDGLDLFTGVTMTYTDSNAGSDTFSGKTKSVGAGLYASALFDSGAYIDLIGKY
                     VHHDNEYTASFAGLGTKDYSSHSWYAGAEVGYRYHVTEDAWIEPQAELVYGAVSGKQF
                     SWKDQGMNLTMKDKDFNPLIGRTGVDVGKSFSGKDWKVTARAGLGYQFDLLANGETVL
                     RDASGEKRIKGEKDGRMLMSVGLNAEVRDNIRFGLEFEKSAFGKYNVDNAVNASFRYS
                     F"
     gene            complement(3317..5989)
                     /gene="rcsD"
                     /locus_tag="ACHKBL_004190"
     CDS             complement(3317..5989)
                     /gene="rcsD"
                     /locus_tag="ACHKBL_004190"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase RcsD"
                     /protein_id="HFO5820972.1"
                     /translation="MRQKETTATTRFSLLPGSITRFFLLLIIVLLVTMGVMVQSAVNA
                     WLKDKSYQIVDITHAIQKRVDTWRYVTWQIYDNIAATTTPSSGEGLQETRLKQDVYYL
                     EKPRRKTEALIFGSHDNSTLEMTQRMSTYLDTLWGAENVPWSMYYLNGQDNSLVLIST
                     LPLKDLTSGFKESTVSDIVDSRRAEMLQQANALDERESFSNMRRLAWQNGHYFTLRTT
                     FNQPGHLATVVAFDLPINDLIPPGMPLDSFRLEPDATATGDNDNEKEGTDSVSIHFNS
                     TKIEISSALNSTDMRLVWQVPYGTLLLDTLQNILLPLLLNIGLLALALFGYTTFRHFS
                     SRSTESLPNTAVNNELRILRAINEEIVSLLPLGLLVHDQESNRTVISNKIADHLLPHL
                     NLQNITTMAEQHQGIIQATINNELYEIRMFRSQVAPRTQIFIIRDQDREVLVNKKLKQ
                     AQRLYEKNQQGRMTFMKNIGDALKEPAQSLAESAAKLNAPEGKQLTNQADVLVRLVDE
                     IQLANMLADDSWKSETVLFSVQDLIDEVVPSVLPAIKRKGLQLLINNHLKAHDMRRGD
                     RDALRRILLLLMQYAVTSTQLGKITLEVDQDESSEDRLTFRILDTGEGVSIHEMDNLH
                     FPFINQTQNDRYGKADPLAFWLSDQLARKLGGHLNIKTRDGLGTRYSVHIKMLAADPE
                     VEEEEERLLDDVCVMVDVTSAEIRNIVTRQLENWGATCITPDERLISQDYDIFLTDNP
                     SNLTASGLLLSDDESGVREIGPGQLCVNFNMSNAMQEAVLQLIEVQLAQEEVTESPLG
                     GDENAQLHASGYYALFVDTVPDDVKRLYTEAATSDFAALAQTAHRLKGVFAMLNLVPG
                     KQLCETLEHLIREKDVPGIEKYISDIDSYVKSLL"
BASE COUNT         1575 a         1473 c         1783 g         1608 t
ORIGIN      
        1 cggacttgcg ggagacgcac ttcataaaat tggtaaggga acactggtgg tggccggcag
       61 tggtgaaaat cctggcaccc tgaatacggg ggacggaacg gtcattctgg cacagaaggc
      121 tgatgctgca ggcagggtcc gggcattcag tgaagtcagt attgtcagcg gacgaccagt
      181 tgtggtgctg caggacagtc atcagattga aggggaccgc attcgctggg gataccgggg
      241 aggtacgctt gatatcaacg gaaatgacat agccttcaac cgcctggctg cggctgatga
      301 gggggcagta ctcacaagcc gggcgcgtcc tgccacagtc cgtctggatt tcagccagtc
      361 agggcagaag gccgtgatgt ggcatggtca ttttaccggt aacctcagcg tcctgaacaa
      421 cacgtcatct gcggtggatt ttatcatgga tggcggagcg gatatatccg gcagttttac
      481 acagcagggc gggggactgt atatccaggg gcatccggtt gttcatgctg tcagcagtga
      541 ggcggttgcg actgcattgc gaaagcaggg tgataattct gttctgacgc agccggtatc
      601 atttgctcag aaggactggg agagcagaac gttcagcatc ggtcaactga aactgaaggg
      661 ggcggcattc agcctttccc gcaatgcgac actgacggga gatattgatg ctgataatgc
      721 caccatggtg ctgggaagtg actcgctata tctggatatg aaggacggca cggggagcag
      781 cagtgcgccg gttaagggga cgtctgccgt gggcggtgca tccggtagca ctacatttcg
      841 cgggaatgtg aatatgcgtc attctgccct gacggtccgc gaccatttta ctggcagtat
      901 aacggcatcg gacagccgga tagctgtgag ttcagagaat gtgcgtctgg aagggaattc
      961 ccgcctgacc tcttctgcac ttactgtcag tgacggcggg cgtttacatg tgaagggggg
     1021 actggagaca gatggtggtg tgacactgga cagaggaacc cttttggtgg acgggggaag
     1081 tgtcaggaat gatgtatatg aacgtctgct ggcctggtca gaggagcgtg gcgggctgaa
     1141 cggaagcggt gagtatgatt ttatgacggg ggcagctggt ctcctgcgga gttatgtcag
     1201 agggagtgca ggtaatgtga atctgcagaa tgcggcctgg atgatgacgg gaaactcatc
     1261 cgtgaagcat cttgagtcat cgggaagtgc tctgtatttc agcaggccgg gcggggagtt
     1321 ccataccctg acggcaggca gtatggatat cagcgacagt gttctggtga tgcggacgga
     1381 ccttaaccac tcagaccagc ttcgggtgac agaatcgctg cggggtaaga ataacctgtt
     1441 gttggttgat tttactgagc gttctgacgg gcagaaggcg ctgaatatcc ctctggtgac
     1501 ggcgccggcc ggtaccggtg cggatgtgtt cagtgtgaaa acgcgtgata ccggcttcag
     1561 tcatatcaca cctgttgtca gggctgaaca ggggacgggc ggcacggcct ggcagctgaa
     1621 tgttgtgcag ccggaaactg ccgcagagcc tgtggcgacg cggcaggatg cagagacgcc
     1681 gaatcctgtg gaagctgtat caccgctccc ttcaccggtt tctgctccgt catcggctcc
     1741 ggaggcgtca gtgacggatg tactgaccgg agagaatgcg ggggaatcag gtgagtaccg
     1801 tgatgagacc cggtggctgc tgacaggata ccggagtacg gttaatagct ccgcggtcag
     1861 ggatgcgggg atgctgatgt caatggggca ccgtaacttc attaatgagg tcaacaacct
     1921 gaacaaacgt atgggtgacc tgcgggacat caacggcgag gccggtgcat gggcccgtat
     1981 catgagcgga accggctctg ccggcggtgg cttcagtgac aactacacac acgttcaggt
     2041 gggggcggat aaaaaacacg aactggacgg tctggacctc ttcaccgggg tgaccatgac
     2101 atataccgac agcaacgccg gcagcgacac cttcagtggt aaaacgaagt cagtgggtgc
     2161 gggtctgtat gcttcagccc tgtttgattc cggggcctat atcgacctga ttggtaagta
     2221 tgttcaccat gataatgagt atacggcgtc ctttgccgga ctcggcacaa aagactacag
     2281 ctctcattcc tggtatgccg gtgctgaagt gggttaccgc tatcatgtca cagaggatgc
     2341 ctggattgag ccacaggcgg aactggtcta tggtgctgtc tccgggaaac agttcagctg
     2401 gaaggaccag ggaatgaacc tcaccatgaa ggataaggac tttaatccgc tgattggccg
     2461 taccggtgtg gatgtgggta aatctttctc cggtaaggac tggaaagtga cggcacgtgc
     2521 tggtcttggc tatcagtttg acctgctggc gaacggagaa acggtactgc gtgatgcatc
     2581 cggagagaag cgtattaagg gcgagaaaga cggccgtatg ctgatgtctg tgggtctgaa
     2641 cgcagaggtc cgtgacaaca tccgttttgg tctggagttt gagaaatcag cctttggtaa
     2701 gtacaacgtt gataatgctg tcaacgccag cttccgttat tcattctgac ggttgttact
     2761 gaaggggatt atggcctgac ggctccctgt aaataaagac aggcacagaa gattgtgagt
     2821 tactcagtgt tccaccaaaa agtaaggtcg caactctgtg cctgcttcgt aagtgtgcag
     2881 tgagggccat atggttataa ctgaagcatc cgacgacgta gtgtctacca gagtgtcaga
     2941 tatgcaggaa aatatgagtt ccgacagaaa tgcagtaaaa aaaggagtct cgcactcccc
     3001 tccataaaac tccagggctg taagttgtat taaaaagctg atttttcttt agcgatacag
     3061 acctcaataa gtataacacc cttcagttac tgtggttatg cacaggtgtt atttttaaca
     3121 gtgtggataa acagtgtcga ttattcagat tggttatggg gcatgtatgg gacattttta
     3181 atcggcgaaa tacgtcgaag ttcgtcgata tggtaaacga atcatctatc caacccttga
     3241 aaaacggcgc tcctggacga tcttcgtcga tttttaaaat gcaacaagta cgttgttcat
     3301 gtaataggct accttgctac agcaagctct tgacataact gtcaatgtcg ctgatgtatt
     3361 tttctattcc tggaacatcc ttctcacgaa tcagatgttc cagcgtttca cataactgct
     3421 tgccgggtac cagatttagc atggcaaata cgcctttaag acgatgagcc gtttgggcta
     3481 acgcagcaaa gtcactggtc gctgcttcag tatacagcct cttaacatca tccggtactg
     3541 tgtctacaaa gagcgcataa tagccgctgg catggagttg cgcattttca tctccgccca
     3601 gaggcgattc tgtcacctct tcctgcgcca gttgcacttc aattaattgt aagaccgctt
     3661 cctgcatagc gttgctcata ttgaagttga cgcacaattg accaggccca atttcccgta
     3721 cgccagactc atcatcgctt aaaagcaagc cagaggcagt aagattagac ggattatccg
     3781 ttaaaaagat atcataatct tgactaatta atctttcatc gggtgtgata caggttgcac
     3841 cccaattttc taactggcga gtgacaatat tccgaatttc tgccgaagta acatccacca
     3901 ttacgcagac atcatccagt aaacgctctt cttcctcttc aacttccggg tcagctgcga
     3961 gcattttgat atgcacagag tagcgagtac caagcccatc ccgcgttttg atgtttaaat
     4021 gaccgcccag tttacgcgcc agttgatcgc tcagccagaa tgccagcggg tccgccttgc
     4081 catagcgatc gttttgggtc tggttgataa acgggaagtg caaattatcc atttcatgaa
     4141 tgcttacgcc ttctccagtg tccagaatac ggaacgtcag gcggtcttcg gaggactcat
     4201 cctgatcaac ctcaagggtg atttttccca attgcgttga ggtcacggca tattgcatca
     4261 gtaacagcaa aatacgtcgt aaggcatcgc gatcgccgcg gcgcatgtcg tgtgctttca
     4321 gatgattgtt aatcagcagt tgcagacctt tacgcttgat ggcaggcaac actgaaggca
     4381 caacttcatc aattaaatcc tgcacggaga acagcaccgt ctcacttttc cagctatcgt
     4441 ccgcaagcat gttcgctaac tgtatttcat cgaccagccg caccagcaca tccgcctgat
     4501 tcgtcagttg tttgccttcc ggggcgttga gtttagccgc gctctccgcc agggactgtg
     4561 cgggttcttt cagcgcatcg ccaatgtttt tcataaaggt catccgcccc tgctggtttt
     4621 tctcatacag acgctgcgcc tgcttgagtt tcttgtttac cagcacttcg cgatcctgat
     4681 cgcgaataat gaaaatttgt gtgcgcggcg caacctggct gcggaacatg cggatctcat
     4741 acagctcgtt attgatcgtc gcctgaataa tcccctgatg ctgttccgcc atggtggtga
     4801 tgttttgcag attcaaatgc ggcagcaaat gatcggcaat tttgttactt atgacagtgc
     4861 ggttcgattc ctgatcgtga accagcaggc cgagcggcag cagtgagact atctcttcat
     4921 tgattgcccg taaaatgcgc aattcgttat tgaccgccgt gttgggtaga ctttctgtac
     4981 tgcgactgga gaaatggcga aatgtggtat agccaaataa cgccagcgcc agcaaaccga
     5041 tgttcagcag cagtggcagc agaatgtttt gcaacgtatc cagcaataag gtgccataag
     5101 gaacctgcca gaccaggcgc atatcggtag agttgagtgc cgaggagatt tcaatcttcg
     5161 tactgttaaa gtggatactg acgctatccg tcccttcttt ctcattatca ttgtctcccg
     5221 ttgccgtcgc gtctggctca aggcggaaac tgtccagcgg catacccggt gggatcaaat
     5281 cattaatcgg cagatcaaaa gccacaaccg ttgccagatg tcccggctgg ttgaacgtgg
     5341 tacgcaaggt aaagtaatga ccgttctgcc aggccaggcg gcgcatgtta gaaaagcttt
     5401 cgcgttcatc gagggcgttg gcctgttgca acatctctgc acgacgtgaa tcaacaatgt
     5461 cgctgacggt cgattcttta aatccggagg tgagatcttt gaggggcagg gttgagatca
     5521 gcaccagact gttatcctga ccattcaggt aatacatcga ccacggtaca ttttctgcgc
     5581 cccacaatgt atccagataa gtggacatcc gttgagtcat ctcaagcgtt gagttgtcgt
     5641 gagagccaaa gattaacgct tccgttttgc ggcgtggttt ctccagatag tagacatcct
     5701 gtttcaggcg cgtctcttgt aaaccttcgc cggaggaggg ggtggtcgtc gcggcaatgt
     5761 tgtcgtagat ctgccaggtc acgtaacgcc aggtatcgac gcgcttttgg atagcgtggg
     5821 taatgtcgac aatctggtaa cttttatctt tcagccaggc gttaacggcg ctttgtacca
     5881 ttacacccat cgtcaccagt aacacaatga tcaacagtaa aaagaagcgg gtaatgctcc
     5941 ccggtaggag tgaaaagcgg gtcgtggccg ttgtctcttt ctgacgcatt cgtgtttatg
     6001 acctgttaaa acttcgcgaa gtgatagggc agtataaagg gtacagtgtg aaattccaga
     6061 ctcttaccgg attgatgatt ctctattctc ccgtgatatt tatcatcctg cattggggga
     6121 aatagatgta caagcgccat tttgctgtac aaataaccta caaaaagcct aacgaaatta
     6181 attgcaaata aacgaagatt gctggaaatt atgctgatgt taattatttg tgaaatagtt
     6241 aacaagcgtt atagtttttc tgtggtagca cagaataatg aaaagtgtgt aaagaagggt
     6301 aaaaaaaacc ggatgcgagg catccggttg aaataggggt aaacagacat tcagaaatga
     6361 atgacggtaa taaataaagt taatgatgat agcggaagat attctagttg cgagtgaagg
     6421 ttttgttttg acattcagt
//
LOCUS       DAZFLD010000228         6418 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.229, whole genome shotgun sequence.
ACCESSION   DAZFLD010000228 DAZFLD010000000
VERSION     DAZFLD010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6418
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.229"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            67..1983
                     /gene="mngA"
                     /locus_tag="ACHKBL_004191"
     CDS             67..1983
                     /gene="mngA"
                     /locus_tag="ACHKBL_004191"
                     /EC_number="2.7.1.195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001393949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HFO5820973.1"
                     /translation="MNLTTLTHRDALCLNARFTSREEAIHALTQRLAALGKISSTEQF
                     LKEVYRRESLGPTALGEGLAVPHGKTAAVKEAAFAVATLSEPLQWEGVDGPEAVDLVV
                     LLAIPPNEAGTTHMQLLTALTTRLADDEIRARIQSATTPDELLSALDDKGGTQPSASF
                     SNAPTIVCVTACPAGIAHTYMAAEYLEKAGRKLGVNVYVEKQGANGIEGRLTAEQLNS
                     ATACIFAAEVAIKESERFNGIPALSVPVAEPIRHAEALIQQALNLKRSDETRTVQQDT
                     QPVKSLKTELKQALLSGISFAVPLIVAGGTVLAVAVLLSQIFGLQDLFNEENSWLWMY
                     RKLGGGLLGILMVPVLAAYTAYSLADKPALAPGFAAGLAANMIGSGFLGAVVGGLIAG
                     YLMRWVKNHLRLSSKFNGFLTFYLYPVLGTLGAGSLMLFVVGEPVAWINNSLTAWLNG
                     LSGSNALLLGAILGFMCSFDLGGPVNKAAYAFCLGAMANGVYGPYAIFASVKMVSAFT
                     VTASTMLAPRLFKEFEIETGKSTWLLGLAGITEGAIPMAIEDPLRVIGSFVLGSMVTG
                     AIVGAMNIGLSTPGAGIFSLFLLHDNGAGGVMAAIGWFGAALVGAAISTAILLIWRRH
                     AVKHGNYLTDGVMP"
     gene            2001..4634
                     /gene="mngB"
                     /locus_tag="ACHKBL_004192"
     CDS             2001..4634
                     /gene="mngB"
                     /locus_tag="ACHKBL_004192"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000648955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HFO5820974.1"
                     /translation="MKAVSRVHITPHMHWDREWYFTTEESRILLVNNMEEILCRLEQD
                     NEYKYYVLDGQTAILEDYFAVKPENKDRVKKQVEAGKLIIGPWYTQTDTTIVSAESIV
                     RNLMYGMRDCLTFGEPMKIGYLPDSFGMSGQLPHIYNGFGITRTMFWRGCSERHGTDK
                     TEFLWQSSDGSEVTAQVLPLGYAIGKYLPADEDGLRKRLDSYFDVLEKASVTKEILLP
                     NGHDQMPLQQNIFEVMDKLREIYPQRKFVMSRFEEVFEKIEAQRDNLATLKGEFIDGK
                     YMRVHRTIGSTRMDIKIAHARIENKIVNLLEPLATLAWTLGFEYHHGLLEKLWKEILK
                     NHAHDSIGCCCSDKVHREIVARFELAEDMADNLLRFYMRKIADNMPQSDADKLVLFNL
                     MPWPREEVINTTVRLRASQFNLRDDRGQPVPYFIRHAREIDPGLIDRQIVHYGNYDPF
                     MEFDIQINQIVPSMGYRTLYIEANQPGNVIAAKSDAEGILENAFWQIALNEDGTLQLV
                     DKDSGVRYDRVLQIEESSDDGDEYDYSPAKEEWVITSANAKPQCEITHEAWQSRAVIR
                     YDMAVPRNLHERSARQSTGRVGVELAVTLSDNSRRIDVDINLDNQADDHRLRVLVPTP
                     FNTDSVLADTQFGSLTRPVNDSAMNNWQQEGWKEAPVPVWNMLNYAALQEGRNGLAVF
                     SEGLREYEVIGEEKKTFAITLLRGVGLLGKEDLLLRPGRPSGIKMPVPDSQLRGSFSC
                     RLSLFSYTGTPSTAGVAQQARAWLTPVQCYNKIPWDAMKLNKAGFNVPESYSLLKMPP
                     VGCLISALKKAEDRQEVVLRLFNPAESTTCEATVAFSREVISCTQTMMDESISTAERD
                     NLKELGAFLPGQSRTFSYRLG"
     gene            complement(4779..6176)
                     /locus_tag="ACHKBL_004193"
     CDS             complement(4779..6176)
                     /locus_tag="ACHKBL_004193"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001577995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HFO5820975.1"
                     /translation="MHHLSRLDKAFLRKGIQLSRANNIVSLSRPSGASARVLLPDELP
                     LEEKAVTQLLDFAEVKLPDHPGYVRQTCATPDFHPGNGVPVGAVVASTPDLVIPAAIG
                     TDISCGMRLLTTGLTLEQAEAHKDVIAQRLTQVLLQNERDVPLSAQAFTALFDGGPAD
                     CVSTLPSEGLWIRTNIPRMVQEIDRCIGLNEHNSHSRYAPPALQATDRALLRDPCLGT
                     PGSGNHFIELQVVDSVVDRHVAWQQGLKTGDIVVMIHSGSRDVGFYVGRHWMDRARAE
                     WPTGHKHPQSGLYGLAGGLAEEYLQAIGMAARYAWLNRVTIAEMVRVCLANLFHQDNS
                     HLVVDVPHNIILREHEMNIHRKGATPARVGNLALIPGSMGDYSWLAVGCGNPEWLWSC
                     SHGAGRSQRRQAMRSRATEESTLPWQCVTLREERRFEEAPAAYKDIGPVIEAQQEAGL
                     IQPAVRFRPWLTFKG"
BASE COUNT         1553 a         1547 c         1766 g         1552 t
ORIGIN      
        1 cacataatgg tattgtttta tcgggcacac tggcgcgact ataaaaacga tcaagtgagg
       61 atcatgatga acctgacgac tctgacccac cgcgatgcgt tgtgtctgaa tgcgcgcttt
      121 accagccgtg aagaggccat ccacgcgttg actcaacgtc ttgctgctct ggggaaaatt
      181 tccagtactg agcaatttct gaaagaagtg tatcgccgtg aaagccttgg cccgaccgcc
      241 ttaggtgaag ggctggctgt gccgcatggc aaaactgctg cggtaaaaga agcggcgttt
      301 gcggtcgcca cactcagcga gccgcttcag tgggaaggcg tggatggccc ggaagcggtt
      361 gatttagtgg tgttgctggc gattcccccc aatgaagcgg gtactacgca catgcaactg
      421 ctgacagcgc tgaccacgcg cctggcggat gatgagatac gggcgcgtat acagtcggcg
      481 acgacgcctg atgaattgct ctcggcgctg gatgacaagg gaggcacgca accctcagct
      541 tctttttcca acgcgccaac tatcgtctgc gtaacggcct gcccggcggg tattgctcac
      601 acctatatgg ctgcggagta tctggaaaaa gccggacgca aactcggcgt gaatgtctac
      661 gttgaaaagc aaggtgctaa cggcattgaa gggcgtttaa cggcagagca actcaatagt
      721 gcaaccgcct gtatttttgc ggctgaagtc gccatcaagg agagtgagcg ttttaacggc
      781 attcccgcgc tttctgtgcc tgttgccgag ccgattcgcc atgcagaagc gttgatccaa
      841 caagcgctta acctcaagcg tagcgatgag acgcgtaccg tacagcaaga tacgcaaccg
      901 gtgaaaagcc tcaaaacgga gctaaaacag gcactgttga gcgggatctc ttttgcagtg
      961 ccgttgattg tcgcgggggg cacggtgctg gcggttgcgg tattactgtc gcaaatcttc
     1021 gggctacaag atttgtttaa tgaagaaaac tcctggctgt ggatgtaccg caaactgggc
     1081 ggagggctgc tcgggatttt gatggtaccg gtgctggcag cctacaccgc ctattcactg
     1141 gctgataaac cggcgttagc gccaggcttt gcggctggac ttgccgccaa catgatcggt
     1201 tccgggtttc tcggtgctgt cgttggcgga ttgatagccg gttatctgat gcgctgggtg
     1261 aaaaatcact tgcgtcttag cagtaaattc aatggattcc tgacttttta tctctacccg
     1321 gtgctcggta cgttgggagc gggcagtctg atgctgtttg tggtggggga acctgtcgcc
     1381 tggatcaata actcgcttac cgcctggctg aacggtctgt caggaagtaa cgcgctgttg
     1441 ctgggtgcca ttctcggttt tatgtgttcc tttgaccttg gagggccagt gaacaaagcc
     1501 gcttatgcat tctgcctggg cgcaatggcg aacggcgttt acggcccgta tgccattttc
     1561 gcctccgtca aaatggtttc ggcatttacc gtaaccgctt ccacgatgct cgcaccgcgc
     1621 ctgtttaaag agtttgaaat tgagaccggg aaatccacct ggctgttagg gctggcaggt
     1681 attaccgaag gggcgatccc gatggcgatt gaagatccgc tgcgggttat tggttcgttt
     1741 gtgctgggct ctatggtaac gggcgctatc gtcggcgcga tgaatatcgg cctttcgaca
     1801 cccggtgccg gcattttctc gctcttttta cttcatgata atggcgcggg cggtgttatg
     1861 gcggcaattg gctggtttgg tgcggcattg gtgggggctg caatctcgac tgcaattctt
     1921 ctgatctggc ggcgtcacgc ggttaagcat ggcaactatc tgactgatgg cgtaatgcca
     1981 taaacaaaaa ggaaacgacg atgaaagcgg tatctcgcgt tcacatcacc ccgcatatgc
     2041 actgggatcg ggagtggtat ttcaccaccg aagagtcacg cattctgctg gtcaataata
     2101 tggaagagat cctgtgccga ctggaacagg acaacgaata caaatattac gtactcgacg
     2161 ggcaaacggc gatcctcgaa gattatttcg cggtgaaacc ggaaaacaaa gaccgtgtga
     2221 agaaacaggt agaagcaggc aagttgatta tcggcccctg gtatacccag accgatacca
     2281 cgattgtttc tgcggaatcc atcgtccgta atctgatgta cggaatgcgt gactgcctca
     2341 cgtttggcga gccgatgaaa ataggttatt taccagattc ctttggcatg tccgggcaac
     2401 tgccgcatat ttacaatgga tttggcatta cccgcaccat gttctggcgc ggatgttctg
     2461 agcgccacgg tactgataaa accgagtttt tgtggcaaag cagtgacggt agcgaagtga
     2521 cggcgcaggt gctgccgctg ggctacgcca tcggtaagta cttacctgcc gacgaagacg
     2581 gattacgcaa acgcctcgac agttattttg atgtgctgga aaaagcgtca gtaaccaaag
     2641 agattttgct gccgaatggt catgaccaga tgccattgca gcaaaatatc ttcgaagtga
     2701 tggataagct acgtgagatc taccctcaac gtaagtttgt gatgagccgc tttgaagagg
     2761 tatttgagaa gatcgaagcg cagcgagata atctggcaac cctgaaaggg gaatttattg
     2821 atggcaaata tatgcgcgtg catcgcacca tcggttctac gcgtatggat atcaaaattg
     2881 cccacgcgcg tattgaaaat aagattgtta atctgctgga accgctggca acgctggcct
     2941 ggacgttggg ttttgaatac caccacggct tgctggagaa attgtggaaa gaaattttaa
     3001 aaaaccacgc ccatgacagc atcggctgct gctgtagtga caaagttcat cgcgagattg
     3061 tcgcccgttt cgaactggca gaagacatgg cggataatct gctgcgtttc tatatgcgta
     3121 aaattgccga taacatgcca cagagtgatg ccgacaaact tgtgctgttt aacctgatgc
     3181 cctggccgcg ggaagaagtg atcaacacca ctgtgcggct gcgcgccagc cagtttaatt
     3241 tgcgggacga tcgcggtcag cctgtaccgt attttattcg ccatgcccgt gagatcgatc
     3301 ctggcctaat cgatcggcaa atcgttcatt acggtaatta tgatcccttt atggagtttg
     3361 atattcagat caaccagatt gtcccttcta tgggctatcg cacgctttat atcgaagcga
     3421 atcagcctgg caacgtgatt gcggcaaaaa gtgacgctga agggatactg gaaaatgctt
     3481 tctggcaaat tgcgctcaat gaggatggaa cgctacaact ggtagataaa gacagcggtg
     3541 tgcgctatga ccgggtgttg caaattgaag aaagctctga tgatggtgat gaatatgact
     3601 attcacccgc aaaagaagag tgggtaatta cttcggcgaa cgcgaaaccg caatgcgaga
     3661 ttactcatga ggcctggcag agtagggctg ttatccgcta tgacatggcg gtgccgcgta
     3721 atctgcatga acgcagcgca agacaatcca ctggcagagt aggggtggag ttggctgtca
     3781 ctctgagtga taacagcagg cgcattgatg tggatatcaa ccttgataac caggctgacg
     3841 atcatcgcct tcgcgtcctg gtccctacac cttttaacac cgacagtgtt ctggccgata
     3901 cccagtttgg ttcgctaacg cgccccgtga acgacagtgc aatgaacaac tggcagcaag
     3961 aaggctggaa agaagcgccg gttccggtat ggaatatgct caactatgct gccttacagg
     4021 aagggcgtaa cggtctggct gtctttagcg aagggctacg tgaatatgaa gtcatcggtg
     4081 aagagaagaa aacctttgcc attacgttgc tgcgtggcgt gggcttactg gggaaagaag
     4141 atctgctttt aaggcctgga cgtccttcag gaataaaaat gccggtgccc gattcacaat
     4201 tacgtggctc ctttagctgc cgtctgagct tatttagtta tacaggtacg ccttcgaccg
     4261 ccggtgtagc ccagcaggcg cgcgcatggc tgacgccagt acagtgttac aacaaaatcc
     4321 cgtgggatgc gatgaagtta aataaagccg gattcaatgt gccggaaagc tatagcttgc
     4381 tgaaaatgcc ccctgtggga tgcctgatta gcgctcttaa aaaagcggaa gaccggcaag
     4441 aagtggtatt gcgactgttt aatccggcag aatcgacaac ctgtgaggca actgtcgcgt
     4501 tcagtcgtga ggtgatttca tgtacgcaaa cgatgatgga tgaaagcatc agcacagcgg
     4561 aaagagataa tttaaaggaa ctgggggcat ttttaccggg gcagtcacgg acatttagct
     4621 accgccttgg ctgattattg cgctaaaagt gtatctgagg ggggcaataa tttcaggcca
     4681 tcttcggatg gcctgagatt gttgataaac aagtttgtta gcttacgcag ataccagatt
     4741 tgacctgcgt gagaaccgca ggccatttat catttcagtt accccttaaa cgttaaccac
     4801 ggtcgaaatc tgaccgctgg ctgaattaat ccggcttctt gttgggcttc gataaccgga
     4861 ccaatatctt tataagccgc tggcgcttct tcaaaacgtc gttcttcgcg caatgtgacg
     4921 cattgccacg gcagcgttga ctcttcagtc gcgcgtgagc gcatggcctg ccgacgctgg
     4981 ctgcgtcctg ctccgtgact gcatgaccac agccactccg ggttgccgca acctaccgca
     5041 agccacgaat aatcgcccat tgaaccgggg attaacgcca gattgcccac tcgtgccggt
     5101 gttgcgcctt tgcgatgaat attcatttca tgctcgcgaa ggatgatgtt atgtggcaca
     5161 tccaccacca gatgcgaatt gtcctgatga aagagattgg caagacatac gcgcaccatt
     5221 tcggcaattg tgacgcgatt taaccatgcg taacgcgccg ccattcctat cgcttgtaga
     5281 tattcctccg ccagtccgcc tgccagacca tataacccgc tttgtggatg tttatgtccg
     5341 gtgggccatt cagcacgggc gcgatccatc cagtgtcttc cgacgtaaaa gccaacatct
     5401 cgcgatccac tgtggatcat caccacaata tcgcctgttt tcagcccttg ttgccaggct
     5461 acatggcgat caaccacgct atcaacgacc tgaagttcaa taaaatgatt acctgaaccc
     5521 ggcgtaccca gacaaggatc gcggagtaat gcgcggtccg ttgcctgcag ggcgggtggg
     5581 gcataacgcg aatgactgtt atgctcgtta agcccaatgc agcggtcaat ctcttgtacc
     5641 attcggggga tattcgtgcg tatccagaga ccttctgatg gcaaggtcga cacgcaatct
     5701 gccggtccgc catcaaaaag cgcggtgaac gcctgtgcgg ataacggaac atcgcgctca
     5761 ttttgcagta gcacttgcgt taaccgctga gcgatgacgt ctttatgcgc ttcggcttgc
     5821 tcaagtgtta atccggtcgt cagcagccgc atgccacaag aaatatcggt gccgatggcg
     5881 gcgggaatga ctaaatccgg cgttgaggcc accaccgcgc caacggggac gccattgccg
     5941 ggatgaaaat ctggcgtcgc gcacgtctgg cgcacataac ctgggtggtc tggtagcttc
     6001 acttctgcaa aatcaagcag ttgtgttact gctttttctt ccagcggcag ttcgtccggc
     6061 agcagtacgc gggcactggc acctgatgga cgagataatg aaacaatatt gttggcgcgg
     6121 gaaagttgga tccctttgcg cagaaaggct ttatcaagcc ttgaaagatg atgcataacg
     6181 ctgtcctgaa atatagacgt taaacaaccc cctgtatggc gagggtaatt ttgtctttcc
     6241 ggtcagcagc cagcaaaagg gaaagaaaac gcgcgtagtg tatgtggcga tagcaagtgt
     6301 tacaagtggt aaatctggct tattgttaaa gaggaattat cttttgtgaa gtcttttaag
     6361 gccatgactc attcaattta accggacata gataatatat aacatagggt atctaaat
//
LOCUS       DAZFLD010000229         6311 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.230, whole genome shotgun sequence.
ACCESSION   DAZFLD010000229 DAZFLD010000000
VERSION     DAZFLD010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6311
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.230"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            13..927
                     /gene="shuT"
                     /locus_tag="ACHKBL_004194"
     CDS             13..927
                     /gene="shuT"
                     /locus_tag="ACHKBL_004194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312409.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter substrate-binding protein
                     ShuT"
                     /protein_id="HFO5820976.1"
                     /translation="MPRIITRPFLFSPLTLCISAVASAAKTAVKRKKLFTAVLALSWA
                     FSVTATERIVVAGGSLTELIYAMGAGERVVGVDETTSYPPETAKLPHIGYWKQLSSEG
                     ILSLRPDSVITWQDAGPQIVLDQLRAQKVNVVTLPRVPATLEQMYANIRQLAKTLQVP
                     EQGEALVTQINQRLERVQQNVAAKKAPVKAMFILSAGGSAPQVAGKGSVADAILSLAG
                     AENVATHQQYKSYSAESLIAANPEVIVVTSQMVDGDINRLRSIAGITHTAAWKNQRII
                     TVDQNLILGMGPRIADVVESLHQQLWPQ"
     gene            947..2284
                     /gene="hutW"
                     /locus_tag="ACHKBL_004195"
     CDS             947..2284
                     /gene="hutW"
                     /locus_tag="ACHKBL_004195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme anaerobic degradation radical SAM
                     methyltransferase ChuW/HutW"
                     /protein_id="HFO5820977.1"
                     /translation="MNTNNTLDLTPHFALDGDQPFKDRRAMMPFRGAIPVAKEQLTQT
                     WQEMLNQTVSPRKRLVYLHIPFCATHCTFCGFYQNRFNDDACAHYTDALIREIEMEAD
                     SVLHQSAPIHAVYFGGGTPSALSAHDLARIITTLREKLPLAPDCEITIEGRVLNFDAE
                     RIDACLDAGANRFSIGIQSFNSKIRKKMARTSDGPTAIAFMESLVKRDRAAVVCDLLF
                     GLPGQDAQTWGEDLAIARDIGLDGVDLYALNVLPNTPLGKAVENGRTTVPSPAERRDL
                     YLQGCDFMDDAGWRCISNSHWGRTTRERNLYNLLIKQGADCLAFGSGAGGSINGYSWM
                     NERNLQTWHESVAAGKKPLMMIMRNAERNAQWRHTLQSGIETARVPLDELTPHAEKLA
                     PLLAQWHQKGLSRDASTCLRLTNEGRFWASNILQSLNELIQVLNAPAIALEKP"
     gene            2297..2791
                     /gene="hutX"
                     /locus_tag="ACHKBL_004196"
     CDS             2297..2791
                     /gene="hutX"
                     /locus_tag="ACHKBL_004196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme utilization cystosolic carrier protein
                     HutX"
                     /protein_id="HFO5820978.1"
                     /translation="MSHVSLQEFLKTEPDGTLEAIAEQYNTTLLEVVKNLPSPTVVSG
                     DKFDTVWDTVCEWGNVTTLVHTTDVILEFSGELPSGFHRHGYFNLRGKHGMSGHIKAE
                     NCTHIALIERKFMGMDTASILFFNKEGSAMLKIFLGRDDHRQLLSEQVSAFHALAASL
                     KEHA"
     gene            2791..3414
                     /gene="chuY"
                     /locus_tag="ACHKBL_004197"
     CDS             2791..3414
                     /gene="chuY"
                     /locus_tag="ACHKBL_004197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobilin reductase"
                     /protein_id="HFO5820979.1"
                     /translation="MTPWLLFGAGGKGVGARTLELALAEQRPVVAVIRHADAATKLAQ
                     QGVQVFTGDACDASVVAAACRAAGPDALIISTMGGAQDYLAHRTVIDEAEKAGITRMI
                     LVTSLGCGDSWPFLSERAKAAFGQAVREKTLAESWLQTSQLDYAILRPGGLLDGAATG
                     KAQRIQNQECHGFINRADVAAHIHELANAPALNQQVYSLIEPDLKPA"
     gene            3463..4455
                     /gene="shuU"
                     /locus_tag="ACHKBL_004198"
                     /gene_synonym="chuU"
     CDS             3463..4455
                     /gene="shuU"
                     /locus_tag="ACHKBL_004198"
                     /gene_synonym="chuU"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000910402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter permease ShuU/ChuU"
                     /protein_id="HFO5820980.1"
                     /translation="MLKDSLSSARVFMGLSLLLLALVLFGASQGALKISFDALFDEEY
                     RDIWLNIRLPRVLLAVLVGAALATAGVIMQGLFRNPMADPGLLGVSSGSALMVGVAIV
                     LPFSFPVVLVLYEQMVFAIAGSLVVCTIIFLITQRHRDGSMMQLLLAGIAINALCGAA
                     IGILSYIGDEQQLRQLTLWMMGNLGQAQWPTLLVASSFILPAIIATTCLAGTLNLLQL
                     GDEEAHYLGVNVKRKRQQLLLVSSLLVGAAVSVSGIIGFIGLVIPHLIRMTTGANHRW
                     LIPCSALAGACLLLMADTLARTLVQPAEMPVGLLTSLLGGPYFMWLILRNRRIT"
     gene            4452..5222
                     /locus_tag="ACHKBL_004199"
     CDS             4452..5222
                     /locus_tag="ACHKBL_004199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter ATP-binding protein"
                     /protein_id="HFO5820981.1"
                     /translation="MISAQNLVYSLQGRRLTDNVSLTFPGGEIVAILGPNGAGKSTLL
                     RQLTGYLQPDSGECRLFNKPLNEWSITELAKHRAVMRQNSHMAFPFSVQEVIQMGRHP
                     HRTGNQDNETAQIMALCDCQALANRDYRQLSGGEQQRVQLARLLVQLWEPTPSPKWLF
                     LDEPTSALDIHHQQHLFRLLRQLVHERQFNVCCVLHDLNLAARYADRIVLMQKGKVIA
                     NGKPQDVLTQQALTMLYGADITVLKDPANHSPLIVLDH"
     gene            complement(5274..5921)
                     /gene="yhiD"
                     /locus_tag="ACHKBL_004200"
     CDS             complement(5274..5921)
                     /gene="yhiD"
                     /locus_tag="ACHKBL_004200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HFO5820982.1"
                     /translation="MTAEFIIRLILAVIACGAIGMERQMRGKGAGLRTHVLIGMGSAL
                     FMIVSKYGFADVLSLGHVGLDPSRIAAQVVTGVGFIGAGNILVRNQNIVGLTTAADIW
                     VTAAIGMVIGSGMYELGIYGSVMTLLVLEVFHQLTFRLMNKNYHLQLTLVNGNTVSML
                     DWFKQQKIKTDLVSLQENEDHEVVAIDIQLHATTSIEDLLRLLKGMAGVKGVSIS"
     gene            complement(5985..>6311)
                     /gene="hdeB"
                     /locus_tag="ACHKBL_004201"
     CDS             complement(5985..>6311)
                     /gene="hdeB"
                     /locus_tag="ACHKBL_004201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeB"
                     /protein_id="HFO5820983.1"
                     /translation="MNISSLRKAFIFMGAVAALSLVNAQSALAANESAKDMTCQEFID
                     LNPKAMTPVAWWMLHEETVYKGGDTVTLNETDLTQIPKVIEYCKKNPQKNLYTFKNQA
                     SNDLPN"
BASE COUNT         1557 a         1620 c         1575 g         1559 t
ORIGIN      
        1 agttctcata ttatgccaag gatcatcacc aggccgtttc ttttttcccc actgacgtta
       61 tgtatttcag ccgtggcctc tgccgcgaag accgcggtaa aacgaaaaaa attgtttacg
      121 gcagtactgg ctcttagctg ggcttttagc gtaacggcta ccgaacgtat cgtggtcgca
      181 ggaggatcgc tgacggagct aatctacgcg atgggcgctg gcgaacgcgt ggttggtgtc
      241 gatgaaacga catcttatcc accagaaacc gccaaactgc cgcatattgg ttactggaaa
      301 caactcagca gcgaaggcat tttgtcactt cgcccggata gcgtaattac ctggcaggat
      361 gcaggaccgc aaattgtgct cgaccaactg cgggcgcaaa aagtcaacgt cgtcactctg
      421 ccgcgtgtac cagccaccct tgagcagatg tacgccaaca ttcgccaact ggcaaaaacg
      481 ttacaggttc ctgaacaagg cgaggcgctg gtgacacaga tcaaccaacg cctggagcga
      541 gtacagcaaa acgtggcggc caaaaaagcc ccggttaaag ccatgtttat tctctccgca
      601 ggcggaagtg cgccacaggt tgccggtaaa ggcagcgtcg cggacgccat tctttcgctt
      661 gccggagcag aaaacgtcgc tacccaccag cagtacaaaa gctacagcgc ggaatcgctg
      721 attgcggcta accctgaagt gattgtcgtc acctcacaaa tggtcgatgg cgatattaat
      781 cggctacgtt ctattgccgg aattacccac actgccgcat ggaaaaacca gcgcattatc
      841 accgttgatc aaaacctgat tctgggaatg ggtccgcgta ttgctgatgt cgttgagtct
      901 ttacaccagc agctttggcc gcaataaatg gatttaaatc agacgaatga acacaaataa
      961 caccctggat ctcacgccac attttgcttt agacggtgat cagccattta aagacaggcg
     1021 cgccatgatg ccgtttcgtg gggccattcc agttgccaaa gagcaactga cacagacctg
     1081 gcaagagatg ctcaatcaaa cggtatcacc acgtaagcga ctggtttatc tacacattcc
     1141 tttttgtgcg acacactgca cgttttgtgg tttttatcag aaccgtttta atgacgatgc
     1201 gtgtgctcat tataccgacg ccctgattcg tgaaatagaa atggaagcag acagcgtatt
     1261 gcatcagtct gcccccatcc acgcggtcta tttcggtggc ggtacgcctt ccgcgctttc
     1321 agcacacgat ttggccagaa tcatcaccac attacgagaa aagctgccac tggcccctga
     1381 ttgtgaaatc actattgaag gccgggtact gaattttgac gctgagcgaa tcgatgcttg
     1441 ccttgatgct ggagcaaacc gcttctcaat tggtattcag tcgtttaaca gcaaaatccg
     1501 caagaaaatg gcccgcacct cggatggccc aaccgctatt gcgtttatgg aaagcctggt
     1561 caaacgcgac cgtgccgcgg tggtctgtga cctgctgttt ggtctgccgg gtcaggatgc
     1621 gcaaacctgg ggagaagatc tggctattgc ccgcgatatc ggtctcgacg gcgtcgatct
     1681 ctatgcgctc aatgtcctgc ccaatacacc gctgggcaaa gccgtggaaa atgggcgtac
     1741 taccgtgccc tctccggcag aacgtcgcga tctttacctg caagggtgtg attttatgga
     1801 cgatgccggt tggcgctgca tcagtaacag ccactggggc cgtaccacac gcgaacgcaa
     1861 tctctataac ctgctgatta aacaaggtgc cgattgtctg gcctttggtt ccggagccgg
     1921 cggttcgatt aatggttact cctggatgaa cgaacgcaat cttcagacct ggcatgaatc
     1981 cgtcgcagca ggtaaaaaac cgctgatgat gatcatgcgt aacgccgaac gtaacgcgca
     2041 gtggcgtcat accttgcagt cgggcattga aacggcgcgt gtaccgctgg acgaacttac
     2101 gccacatgcc gaaaaactcg cgccgttact ggcccagtgg caccaaaaag gcttaagccg
     2161 cgatgcgtca acgtgtctgc ggttgaccaa tgaaggtcgt ttctgggcaa gcaacatttt
     2221 gcagtctctt aatgaactga ttcaggtact taatgcgcca gcaattgcgc ttgaaaaacc
     2281 ataacaggag tgatttatga gccatgtctc gttacaggaa tttttgaaaa cggagccaga
     2341 cggtacgctg gaggcgatag ccgaacaata caacaccacg ctgctggagg tagtgaaaaa
     2401 tctgccctct ccgactgttg tgtcaggtga taaattcgat actgtctggg ataccgtctg
     2461 cgaatggggc aacgtcacca cgttagtaca tactaccgat gtaatactcg aatttagcgg
     2521 cgaactgcct tcgggtttcc atcgccacgg ctatttcaac ctacgcggaa agcacggcat
     2581 gtccgggcat atcaaagcag aaaactgcac gcatattgcc ttgatcgaac gtaaatttat
     2641 gggcatggat accgcctcta ttctcttttt caataaggaa ggcagcgcaa tgctgaaaat
     2701 cttcctcggt agggatgatc atcgtcagct cctgagcgaa caagtcagtg ccttccatgc
     2761 tctggcagca tcgttaaagg aacacgcctg atgacaccgt ggctactctt tggcgcaggc
     2821 ggaaaaggtg tgggtgccag aacgcttgaa ctggcgctgg cggaacaacg tccggtcgtt
     2881 gctgtcattc gtcatgccga tgccgccacg aaactggcgc aacaaggcgt acaggttttt
     2941 acaggcgacg cctgtgatgc cagcgtagtc gcggctgctt gccgcgcagc tggcccggat
     3001 gcgcttatca tctcaacaat gggcggagct caggattatc tggcgcacag aacagtgatt
     3061 gacgaggccg agaaagcagg catcacacgc atgattctgg tgacgtcact gggatgcggc
     3121 gatagctggc cgtttttatc agagcgggca aaggctgcgt ttggtcaggc ggtacgggaa
     3181 aaaacgctgg cggaaagctg gttgcaaacc agccagcttg attatgccat tctccgccct
     3241 ggcggtctgc ttgatggtgc ggcaacgggc aaagcacaac ggatacaaaa tcaggagtgt
     3301 catggtttta ttaaccgtgc tgatgtcgcc gcacatatcc atgaactggc aaatgcgcca
     3361 gcacttaacc agcaggtcta cagtctgatt gaaccggacc tgaaaccggc gtgatccccc
     3421 ggcgcggcat gtccgcgccg taatgtcgta acggagtttt gcgtgctcaa ggattcactc
     3481 tcttccgcca gggtctttat ggggctatca ctattattgc tagctctggt gctgtttggt
     3541 gccagtcagg gagcgttaaa gatcagtttt gatgcccttt ttgatgagga ataccgcgat
     3601 atctggctca atattcgtct accacgggtt ttgctggcgg tgctggtagg tgcagcgttg
     3661 gcaaccgccg gggtaattat gcaggggctt tttcgcaacc caatggctga ccctggatta
     3721 cttggcgtca gtagcggttc tgcattaatg gttggcgttg ctattgtgct gcccttctcc
     3781 ttcccagtgg tgctggtgct ctatgagcaa atggtgtttg ccattgccgg aagtttagtg
     3841 gtctgcacca tcatttttct catcacgcag cgccatcgcg atggcagcat gatgcaatta
     3901 ttactcgccg gtatcgccat caatgccctg tgcggcgcag cgatcggcat cctgagctat
     3961 atcggcgatg agcagcagct acgacaactc acattgtgga tgatgggcaa tcttggacag
     4021 gcgcaatggc cgacgttatt ggttgccagt tcattcatcc taccggccat tatcgcaaca
     4081 acttgtctcg ccggaacgct gaatttactg cagctcggtg atgaagaagc ccattacctc
     4141 ggcgtgaacg ttaagcgtaa acgccagcaa ttactgttag tgagctcact gctcgttggt
     4201 gccgccgtat cggtaagcgg cattatcggc tttattggcc tggtgatccc gcatctgatt
     4261 cgcatgacta ccggagcaaa tcaccgctgg ctaatccctt gctccgccct cgccggagcc
     4321 tgtttattgc tgatggcaga cacgcttgcc cgcacgctgg tacagccagc agaaatgccc
     4381 gtgggattat taaccagcct gcttggtggt ccttatttta tgtggttgat tctgcgcaac
     4441 cggaggatca catgatcagc gcccagaatc tggtttatag ccttcagggg cggcgtctga
     4501 ctgataatgt ctcgctgact tttcccggtg gggaaattgt tgccattctc ggccctaatg
     4561 gtgcgggaaa atctacgctg cttcgccagt taaccggcta tcttcaacct gatagcggcg
     4621 aatgtcggtt gtttaataag ccattaaatg aatggtcgat tactgaactg gcgaaacacc
     4681 gggccgtgat gcgtcaaaat agccacatgg cgtttccatt cagcgtacaa gaagttatcc
     4741 agatggggcg gcatccgcat cgcactggta atcaggataa tgaaacagca caaataatgg
     4801 cgttatgtga ctgtcaggcg ttagcaaacc gcgactatcg gcaattatcc ggtggtgaac
     4861 aacagcgcgt acaattagcg cgcctgctgg tgcaactctg ggaaccaact ccctcaccga
     4921 aatggttatt tcttgatgaa ccaacttcgg cgctggatat tcaccatcag cagcacttgt
     4981 tccgtttatt acgccagtta gttcacgaac ggcagttcaa tgtttgctgc gtattgcacg
     5041 atctaaatct tgccgcgcgt tatgcagacc gtattgtatt aatgcaaaaa ggaaaggtta
     5101 tcgcaaatgg taaaccacag gacgtattga cacagcaggc gctgaccatg ctttacggcg
     5161 cggatattac cgtacttaaa gatcctgcca accattcacc cctgatcgtc ctcgatcatt
     5221 aaaaccaaca ggagtaccgc actggtactc ctgcgaataa ataactatac agattagcta
     5281 attgaaacgc ctttcacccc tgccatacct tttaataatc gcaacagatc ttctatcgac
     5341 gtcgtggcat gaagctggat atcaatggcg accacttcat gatcttcatt ttcctgcaac
     5401 gaaactaaat ccgtttttat tttttgctgc ttaaaccagt cgagcattga taccgtatta
     5461 ccgttcacta atgtcagctg taaatggtaa tttttgttca tcaggcggaa ggttaattga
     5521 tggaagactt ccagcaccag caaggtcatc actgaaccat aaatacccag ttcgtacata
     5581 ccgctgccaa taaccatacc aatggcggcg gtcacccaga tatccgctgc cgtcgtcaga
     5641 cctacaatat tttggttacg aacgagaatg ttacctgcac cgataaaccc gacgcccgtc
     5701 acaacctgag cagcaatacg gctggggtcc agtccgacgt gacctaaaga cagcacgtca
     5761 gcaaaaccat atttcgaaac aatcataaac agggcgcttc ccatgccaat taatacatgt
     5821 gtgcgtaacc ctgctccttt gccgcgcatt tgcctttcca tgccaatagc gccacaggca
     5881 attaccgcaa gtataagtcg tataataaat tctgctgtca tataaaaatg caaaaaggag
     5941 cagcaagatg gctcaacttg ctactccttt ttacttgtac ctcattaatt cggcaagtca
     6001 ttagatgctt gatttttgaa ggtatacaaa tttttctgcg ggtttttctt gcagtattcg
     6061 atcactttag gaatttgagt gagatcggtt tcatttaaag taacggtatc gccaccttta
     6121 tatactgttt cttcatgcag catccaccac gcaactggtg tcattgcttt tggattcaga
     6181 tcaataaatt cctggcaggt catatcttta gcggattcat tggctgccag cgcagattgt
     6241 gcgttcacca gtgacaaagc cgctacagcg cccataaaaa taaacgcttt acggagagat
     6301 gaaatgttca t
//
LOCUS       DAZFLD010000230         6196 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.231, whole genome shotgun sequence.
ACCESSION   DAZFLD010000230 DAZFLD010000000
VERSION     DAZFLD010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6196)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6196)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6196
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.231"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            226..2073
                     /gene="ldtD"
                     /locus_tag="ACHKBL_004202"
     CDS             226..2073
                     /gene="ldtD"
                     /locus_tag="ACHKBL_004202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFO5820984.1"
                     /translation="MLLNMMCGRRLSAISLCLAVTFAPLFNAQADEPEVIPGDSPVAV
                     SEQGEALPQAQATAIMAGIQPLPEGAAEKARTQIESQLPAGYKPVYLNQLQLLYAARD
                     MQPMWENRDAVKAFQQQLAEVAIAGFQPQFNKWVELLTDPGVNGMARDVVLSDAMMGY
                     LHFIANIPVKGTRWLYSSKPYALSTPPLSVINQWQLALDKGQLPTFVVGLAPQHPQYA
                     AMHESLLALLSDTKPWPQLTGKATLRPGQWSNDVPALREILQRTGMLDGGPKITLPGD
                     DTPTDAVVSPSAVTIETAETKPMDKQTTSRSKPAPVVRAAYDNELVEAVKRFQAWQGL
                     GADGAIGPATRDWLNVTPAQRAGVLALNIQRLRLLPTELSTGIMVNIPAYSLVYYQNG
                     NQVLDSRVIVGRPDRKTPMMSSALNNVVVNPPWNVPPTLARKDILPKVRNDPGYLESH
                     GYTVMRGWNSREAIDPWQVDWSTITASNLPFRFQQAPGPRNSLGRYKFNMPSSEAIYL
                     HDTPNHNLFKRDTRALSSGCVRVNKASDLANMLLQDAGWNDKRISDALKQGDTRYVNI
                     RQSIPVNLYYLTAFVGADGRTQYRTDIYNYDLPARSSSQIVSKAEQLIR"
     gene            2254..2802
                     /locus_tag="ACHKBL_004203"
     CDS             2254..2802
                     /locus_tag="ACHKBL_004203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF882 domain-containing protein"
                     /protein_id="HFO5820985.1"
                     /translation="MDKFDANRRKLLALGGVALGAAILPTPAFATLSTPRPRILTLNN
                     LHTGESIKAEFFDGRGYIQEELAKLNHFFRDYRANKIKSIDPGLFDQLYRLQGLLGTR
                     KPVQLISGYRSIDTNNELRARSRGVAKKSYHTKGQAMDFHIEGIALSNIRKAALSMRA
                     GGVGYYPRSNFVHIDTGPARHW"
     gene            2829..3476
                     /gene="gloC"
                     /locus_tag="ACHKBL_004204"
     CDS             2829..3476
                     /gene="gloC"
                     /locus_tag="ACHKBL_004204"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003035823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase GloC"
                     /protein_id="HFO5820986.1"
                     /translation="MNYRIIPVTAFSQNCSLIWCEQTRLAALVDPGGDAEKIKQEVDA
                     SGLTLMQILLTHGHLDHVGAAAELAQHYGVPVFGPEKEDEFWLQGLPAQSRMFGLEEC
                     QPLTPDRWLNEGDTISIGNVALQVLHCPGHTPGHVVFFDDRAKLLISGDVIFKGGVGR
                     SDFPRGDHNQLISSIKDKLLPLGDDVTFIPGHGPLSTLGYERLHNPFLQDEMPVW"
     gene            complement(3527..4717)
                     /gene="aspC"
                     /locus_tag="ACHKBL_004205"
     CDS             complement(3527..4717)
                     /gene="aspC"
                     /locus_tag="ACHKBL_004205"
                     /EC_number="2.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009653304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate transaminase"
                     /protein_id="HFO5820987.1"
                     /translation="MFENITAAPADPILGLADLFRADERPGKINLGIGVYKDETGKTP
                     VLTSVKKAEQYLLENETTKNYLGIDGIPEFGRCTQELLFGKGSALINDKRARTAQTPG
                     GTGALRVAADFLAKNTSVKRVWVSNPSWPNHKSVFNSAGLEVREYAYYDAENHTLDFD
                     ALINSLNEAQAGDVVLFHGCCHNPTGIDPTLEQWQTLAQLSVEKGWLPLFDFAYQGFA
                     RGLEEDAEGLRAFAAMHKELIVASSYSKNFGLYNERVGACTLVAADSETIDRAFSQMK
                     AAIRANYSNPPAHGASVVATILSNDALRAIWEQELTDMRQRIQRMRQLFVNTLQEKGA
                     NRDFSFIIKQNGMFSFSGLTKEQVLRLREEFGVYAVASGRVNVAGMTPDNMAPLCEAI
                     VAVL"
     gene            complement(4902..5990)
                     /gene="ompF"
                     /locus_tag="ACHKBL_004206"
     CDS             complement(4902..5990)
                     /gene="ompF"
                     /locus_tag="ACHKBL_004206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpF"
                     /protein_id="HFO5820988.1"
                     /translation="MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKAVGLHY
                     FSKGNGENSYGGNGDMTYARLGFKGETQINSDLTGYGQWEYNFQGNNSEGADAQTGNK
                     TRLAFAGLKYADVGSFDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
                     YRNSNFFGLVDGLNFAVQYLGKNERDTARRSNGDGVGGSISYEYEGFGIVGAYGAADR
                     TNLQEAQPLGNGKKAEQWATGLKYDANNIYLAANYGETRNATPITNKFTNISGFANKT
                     QDVLLVAQYQFDFGLRPSIAYTKSKAKDVEGIGDVDLVNYFEVGATYYFNKNMSTYVD
                     YIINQIDSDNKLGVGSDDTVAVGIVYQF"
BASE COUNT         1563 a         1521 c         1574 g         1538 t
ORIGIN      
        1 cgaaaactcc gtttctgcac taaaaaagtg gcacattacg gcgcgctttt ctttaaactt
       61 ctttacatta ggttatgtaa aaacgtatcg gcgtttatat actgaagata agcctgatga
      121 gtaacaggct tgctcgtcat actttcgtga gtattggcgt tgtacaggca agtcgtaaaa
      181 taacagcctg gctattcaga gtatgataaa aacagggggc aagggatgtt gcttaatatg
      241 atgtgtggtc gtcggctgtc ggcaatcagt ttgtgcctgg ccgtaacatt cgctccactg
      301 ttcaatgcgc aggccgatga gcctgaagta atccctggcg acagcccggt ggctgtcagt
      361 gaacagggcg aggcactgcc gcaggcgcaa gccacggcaa taatggcggg gatccagcca
      421 ttgcctgaag gtgcggctga aaaagcccgc acgcaaatcg aatctcaatt acccgcaggt
      481 tataagccgg tttatcttaa ccagcttcaa ttgttgtatg ccgcacgcga tatgcaaccc
      541 atgtgggaaa accgtgatgc tgttaaagcc ttccagcaac agctggcaga ggtggcgatt
      601 gccggtttcc agccgcagtt taataaatgg gtagagttac tgaccgatcc tggtgttaac
      661 gggatggcac gcgacgtggt gctctctgat gcgatgatgg gctatctcca tttcattgca
      721 aatattccgg tcaaaggcac tcgctggcta tatagcagta aaccttatgc gctttcaacg
      781 ccgccacttt cggtgattaa ccaatggcag ctggcgctgg ataaaggtca attgcctacg
      841 tttgttgtag gattggcacc gcagcatccg caatatgcgg cgatgcatga atcgttactg
      901 gccttactca gtgacaccaa accgtggccc caactgaccg gcaaagcaac gttgcgccca
      961 ggacagtgga gtaacgacgt accggcgttg cgcgaaatat tgcaacgtac aggcatgttg
     1021 gacgggggac cgaaaattac gctacctggc gatgacacgc caactgacgc ggtagtcagc
     1081 ccatccgctg ttactattga aacagcagaa actaagccga tggacaagca aactacgtct
     1141 cgtagtaaac ctgcgcctgt cgttcgtgcc gcctacgata atgaactggt ggaagccgtt
     1201 aaacgttttc aggcatggca aggattgggg gcagatggtg ctattggccc ggcaacgcgt
     1261 gactggttaa acgtaacgcc cgcccagcgt gctggcgtgt tggcactcaa catccagcga
     1321 ttgcgcttgc tgccaacaga gctttctacc gggatcatgg ttaacattcc agcctattcg
     1381 ctggtctact atcagaacgg caatcaggtg ctggattcgc gagtcattgt cggtcgcccc
     1441 gatcgcaaaa cgccgatgat gagcagtgca cttaacaacg tagtggtaaa cccgccgtgg
     1501 aacgtaccac caactctggc acgcaaagat attctgccga aagtgcgcaa cgatccggga
     1561 tatctcgaaa gccatggcta tacggtgatg cgcggctgga acagcagaga agcgattgac
     1621 ccatggcagg ttgactggtc tacaatcacg gcctcgaatt taccgttccg cttccagcag
     1681 gctccaggcc cacggaactc gctggggcgc tataaattca atatgccgag ttcagaggcc
     1741 atttatttgc atgacacgcc gaaccacaat ctgttcaagc gtgatacacg cgcattgagc
     1801 tcaggctgtg tacgagtgaa taaagcttcc gatctggcga atatgctgtt gcaggatgca
     1861 ggctggaatg acaaacgtat ttctgatgcg ctgaagcagg gcgatacgcg ttacgtcaat
     1921 attcggcagt cgattccggt gaatctctac tatctgacgg cctttgttgg tgcagatggt
     1981 cgtacccagt atcgtacaga tatttacaat tatgatctgc ctgcgcgatc cagctcgcaa
     2041 attgtatcga aagcggaaca attaatcagg taaatgaagc agttcgggga aatcagttgt
     2101 cgtaataatt agcatgattg ggggcaattc tctgcagccc ccgtcactgc tggggttgag
     2161 tcatcttgac gtctgcttta cgggcggtta aggtgcctct tgtgcgccag aagtgcatat
     2221 aaacgataac attgacctgt agacttgatt atcatggaca aattcgacgc taatcgccgc
     2281 aaattgctgg cgcttggtgg cgttgcactc ggtgccgcca tcctgccgac ccctgcgttt
     2341 gcaacactct ctaccccacg cccgcgcatt ttgacactca ataatcttca taccggagag
     2401 tcaatcaaag cggagttttt cgatggcaga ggctatattc aggaagaatt ggcaaaactt
     2461 aaccattttt tccgcgatta ccgcgcgaac aaaataaagt ccatcgaccc aggattattc
     2521 gaccagttgt atcgcctgca agggttgtta ggtacccgca aaccggtgca actcatttcc
     2581 ggttatcgtt ccattgatac caacaatgaa ctacgcgccc gcagccgtgg agtagcgaag
     2641 aaaagctatc acacgaaagg gcaggcgatg gatttccata ttgaaggtat cgcgttaagc
     2701 aatattcgca aagccgcgtt atctatgcgc gcaggtggtg taggatatta cccacgtagt
     2761 aactttgtgc atattgatac cgggccagca cggcactggt agcaattgct taacgaaaca
     2821 ggggcagtat gaactatcgt attattccgg tcaccgcatt ctcccagaac tgttcattga
     2881 tctggtgtga acaaacccgc ctggccgcac tggtcgatcc tggcggcgat gcggaaaaaa
     2941 tcaaacagga agttgacgcc agcggcctga cactgatgca gatcctgctg acgcatggtc
     3001 atctggacca cgttggtgca gcggcggaac tggcgcaaca ttacggcgtg ccggtttttg
     3061 gcccggaaaa agaagatgag ttctggctgc aaggcttgcc tgcgcaaagt cgtatgtttg
     3121 gtctggaaga gtgccagccg ctgacgccgg atcgttggct gaacgaaggc gataccatca
     3181 gcatagggaa tgtggcttta caggtgttac attgccctgg gcatacgccg ggtcatgtcg
     3241 tgttttttga tgatcgggca aagctgctga tttctggcga cgttattttc aaaggcggag
     3301 tagggcgcag tgacttcccg cgtggcgatc ataatcaact gatttcttca atcaaagata
     3361 agttgctgcc actgggggat gacgtgacat ttattccggg tcacggacca ttatccacac
     3421 ttggttatga gcgcctgcat aatcccttcc tgcaagacga aatgcccgtc tggtaaaggc
     3481 gcataaaaaa gcccgctttt taagcgggct tcattgtttt taacgcttac agcactgcca
     3541 caatcgcttc gcacagcgga gccatattat ctggtgtcat cccggccacg tttacgcgac
     3601 cagaagcaac agcatatacg ccaaactctt cgcgcagacg cagcacttgt tcttttgtca
     3661 ggccactgaa ggagaacatg ccgttctgtt tgatgataaa gctgaagtcg cgatttgcgc
     3721 ctttttcctg cagcgtattg acgaacaact gacgcatacg ctgaatacgc tggcgcatat
     3781 cagtcagttc ttgttcccaa atcgcacgta acgcatcgtt gctcaggatg gtggcaacaa
     3841 cagaagcgcc gtgtgctggt gggttagagt agttagcgcg aatcgccgct ttcatttggc
     3901 tgaatgcgcg atcaatggtt tcactgtcag cagcaaccag agtacaagcg ccaacacgct
     3961 cgttgtacag gccaaagttt ttagagtagg aactggcaac aatcagctct ttatgcatag
     4021 ccgcgaaagc gcgcagtcct tcagcatctt cttccagacc acgggcaaac ccctggtaag
     4081 cgaagtcaaa cagcggtaac cagcctttct caacggaaag ttgtgccagt gtctgccatt
     4141 gttccagcgt agggtcgata ccggttgggt tatggcagca gccatggaac agcaccacgt
     4201 cgccagcctg agcttcgttc aggctgttaa tcagtgcatc gaagtcaagg gtgtgatttt
     4261 ccgcatcata ataagcgtat tcacgaactt ccagacctgc agagttaaag acgctcttat
     4321 ggttcggcca gcttgggttg ctcacccaca cacgcttaac gctggtattt tttgccagga
     4381 aatcggcagc cacgcgtagt gcgccagtgc cacccggagt ctgtgccgtg cgagcacgtt
     4441 tgtcattgat cagggcgcta cctttaccaa acagcagttc ctgagtgcag cgaccaaatt
     4501 cagggatgcc gtcaatgccg aggtaatttt tggtggtttc attttcgagc agatactgtt
     4561 cagccttttt cacactggtc agtaccgggg ttttgcccgt ctcatcttta tagacaccaa
     4621 tcccgaggtt aattttgccg ggacgttcat cggcacgaaa cagatcggcc aggcccagaa
     4681 tcgggtcggc aggagcggcg gtaatgttct caaacatgac gaggttccat tatggttaca
     4741 gaagggaagt ccgctatcag ggtaacggga gatttacaaa attccaacta ttactgatga
     4801 aaacgcaggc tgtttttgca agacgtgaga ttgctctgga aggcataaaa aaaacaggac
     4861 caaagtcctg ttttttcggc atttaacaaa gaggtgtgct attagaactg gtaaacgata
     4921 cccacagcaa cggtgtcgtc agaacctacg cccagtttgt tgtcagaatc gatctggttg
     4981 atgatgtagt caacataggt ggacatgttt ttgttgaagt agtaggttgc gcccacttca
     5041 aagtagttaa ccagatcaac atcaccgata ccttctacgt ctttcgcttt agatttggtg
     5101 taagcgatgg acggacgcag accgaaatcg aactggtatt gcgcaactaa cagaacgtct
     5161 tgcgttttgt tggcgaagcc gctgatgttt gtaaatttat tagtgatcgg cgtagcgtta
     5221 cgggtttcac cgtagttcgc tgccaggtag atgttgttcg cgtcatactt cagaccagta
     5281 gcccactgtt cagctttttt accgttgcca agaggttgag cttcttgcag gttggtacgg
     5341 tcagcggcgc cataagcacc aacgatacca aagccttcgt attcgtagct gatagaaccg
     5401 ccaacaccgt cgccgttaga gcggcgtgca gtgtcacgct cgtttttacc caggtactga
     5461 acagcgaagt tcaggccatc aaccagacca aagaagttgg agttacgata ggtagcaacg
     5521 ccgccaacac gaccaacgaa gaagtcatcg ctgtatgccg tgtcaccgcc aaactcaggc
     5581 agcatatcgg tgtaacccag tgcatcataa accacaccgt agttacggcc gtaatcgaaa
     5641 gaaccaacgt cagcgtattt aagacccgcg aatgccagac gcgttttgtt accagtttga
     5701 gcgtcagcgc cttcagagtt gttaccctgg aagttatatt cccactgacc ataaccggtc
     5761 agatcggaat tgatttgagt ttccccttta aaaccaagac gggcataggt catgtcacca
     5821 ttgccaccgt aactgttttc accgttaccc ttggagaaat aatgcagacc gacagcttta
     5881 ccgtacagat ctactttgtt gccatctttg ttatagattt ctgcagcgtt tgcagtacct
     5941 gctactaaca gagcagggac gatcactgcc agaatattgc gcttcatcat tatttattac
     6001 cctcatggtt tttttatgac acctgccact gccgtcaata agttctgtca ataaaaattt
     6061 acggaactat tgatgagagt ttggtgtctt tatgtgtctg caggcatctt tccattcaaa
     6121 ctaacgtttc gctaccgtga aagtgctaca aagataaaga tttggtttca aaaagaaaaa
     6181 atatgtaacc aaaagt
//
LOCUS       DAZFLD010000231         6195 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.232, whole genome shotgun sequence.
ACCESSION   DAZFLD010000231 DAZFLD010000000
VERSION     DAZFLD010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6195
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.232"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            142..1578
                     /gene="aspA"
                     /locus_tag="ACHKBL_004207"
     CDS             142..1578
                     /gene="aspA"
                     /locus_tag="ACHKBL_004207"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008797 - aspartate ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HFO5820989.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            1697..2998
                     /gene="dcuA"
                     /locus_tag="ACHKBL_004208"
     CDS             1697..2998
                     /gene="dcuA"
                     /locus_tag="ACHKBL_004208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuA"
                     /protein_id="HFO5820990.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
     gene            3114..3452
                     /gene="cutA"
                     /locus_tag="ACHKBL_004209"
     CDS             3114..3452
                     /gene="cutA"
                     /locus_tag="ACHKBL_004209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HFO5820991.1"
                     /translation="MLDEKSSNTASVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            3428..5125
                     /gene="dsbD"
                     /locus_tag="ACHKBL_004210"
     CDS             3428..5125
                     /gene="dsbD"
                     /locus_tag="ACHKBL_004210"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HFO5820992.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSQFVPADQAFAFDFQQN
                     QHDLNLTWQIKDGYYLYRKQIRITPEHAKIANVQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVANNAASQPVSVPQQEQPT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLAIVFTLLAMSMFGLFTLQLPSSLQTRLT
                     LMSNRQQGGSPGGVFVMGAIAGLICSPCTTAPLSAILLYIAQSGNMWLGGGTLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPVFLLERVIGDIWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRVVQIILLAAALVSVRPLQDWAFGATHTAQTQTHLNF
                     TQIKTVDELNQALVEAKGKPVMLDLYADWCVACKEFEKYTFSDPQVQKALADTVLLQA
                     NVTANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDRQP"
     gene            5162..5737
                     /locus_tag="ACHKBL_004211"
     CDS             5162..5737
                     /locus_tag="ACHKBL_004211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO5820993.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDAWRRQLMLDETQTAEQKLLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPGHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
     gene            5844..5919
                     /locus_tag="ACHKBL_004212"
     tRNA            5844..5919
                     /locus_tag="ACHKBL_004212"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5877..5879,aa:Phe,seq:gaa)
BASE COUNT         1479 a         1609 c         1567 g         1540 t
ORIGIN      
        1 tacggtagaa atcctcaagc agcatatgat ctcgggtatt cggtcgatgc aggggataat
       61 cgtcggtcga aaaacattcg aaaccacata tattctgtgt gtttaaagca aatcattggc
      121 agcttgaaaa agaaggttca catgtcaaac aacattcgta tcgaagaaga tctgttgggt
      181 accagggaag ttccagctga tgcctactat ggtgttcaca ctctgagagc gattgaaaac
      241 ttctatatca gcaacaacaa aatcagtgat attcctgaat ttgttcgcgg tatggtaatg
      301 gttaaaaaag ccgcagctat ggcaaacaaa gagctgcaaa ccattcctaa aagtgtagcg
      361 aatgccatca ttgccgcatg tgatgaagtc ctgaacaacg gaaaatgcat ggatcagttc
      421 ccggtagacg tctaccaggg cggcgcaggt acttccgtaa acatgaacac caacgaagtg
      481 ctggccaata tcggtctgga actgatgggt caccagaaag gtgaatatca gtacctgaac
      541 ccgaacgacc atgttaacaa atgtcagtcc actaacgacg cctacccgac cggtttccgt
      601 atcgcagttt actcttccct gattaagctg gtagatgcga ttaaccaact gcgtgaaggc
      661 tttgaacgta aagctgtcga attccaggac atcctgaaaa tgggtcgtac ccagctgcag
      721 gacgcagtac cgatgaccct cggtcaggaa ttccgcgctt tcagcatcct gctgaaagaa
      781 gaagtgaaaa acatccaacg taccgctgaa ctgctgctgg aagttaacct tggcgcaaca
      841 gcaatcggta ctggtctgaa cacgccgaaa gagtactctc cgctggcagt gaaaaaactg
      901 gctgaagtca ctggcttccc atgcgtaccg gctgaagacc tgatcgaagc gacctctgac
      961 tgcggcgctt atgttatggt tcacggcgcg ctgaaacgcc tggctgtgaa gatgtccaaa
     1021 atctgtaacg acctgcgctt gctctcttca ggcccacgtg ccggcctgaa cgagatcaac
     1081 ctgccggaac tgcaggcggg ctcttccatc atgccagcta aagtaaaccc ggttgttccg
     1141 gaagtggtta accaggtatg cttcaaagtc atcggtaacg acaccactgt taccatggca
     1201 gcagaagcag gtcagctgca gttgaacgtt atggagccgg tcattggcca ggccatgttc
     1261 gaatccgttc acattctgac caacgcttgc tacaacctgc tggaaaaatg cattaacggc
     1321 atcactgcta acaaagaagt gtgcgaaggt tacgtttaca actctatcgg tatcgttact
     1381 tacctgaacc cgttcatcgg tcaccacaac ggtgacatcg tgggtaaaat ctgtgccgaa
     1441 accggtaaga gtgtacgtga agtcgttctg gaacgcggtc tgttgactga agcggaactt
     1501 gacgatattt tctccgtaca gaatctgatg cacccggctt acaaagcaaa acgctatact
     1561 gatgaaagcg aacagtaatc gtacagggta gtacaaataa aaaaggcacg tcagatgacg
     1621 tgcctttttt cttgtgagca gtaacttaaa aataacaatc taatatcaac ttgttaaaaa
     1681 aacaaggaag gctaatatgc tagttgtaga actcatcata gttttgctgg cgatcttctt
     1741 gggcgccaga ttggggggaa taggtattgg ttttgcaggc ggattggggg tgctggttct
     1801 tgccgctatt ggcgttaaac ccggtaacat cccgttcgat gtcatttcca ttatcatggc
     1861 ggttatcgcc gctatttctg ccatgcaggt tgctggcggt ctggactatc tggttcatca
     1921 gacagaaaag ctgctgcgcc gtaacccgaa atacatcacg atcctcgcac cgatcgtgac
     1981 ctatttcctg acaatctttg ctggtactgg caacatctct ctggcgacac tgccagttat
     2041 cgctgaagtt gcgaaggaac aaggcgttaa accttgccgt ccgctgtcta ctgcagtggt
     2101 atccgcgcag attgcgatca ccgcatcgcc aatctcagcg gcagtggttt acatgtcttc
     2161 cgtaatggaa ggtcatggca tcagctacct ccatctgctc tccgtggtca tcccgtccac
     2221 cctgctggcg gttctggtga tgtccttcct ggtcactatg ctgttcaact ccaaactctc
     2281 tgacgatccg atttatcgca agcgtctgga agaaggcctg gttgaactgc gcggtgaaaa
     2341 gcagattgaa atcaaatccg gtgcaaaaac gtccgtctgg ctgttcctgc tgggcgtagt
     2401 tggcgttgtt atctatgcaa tcatcaacag cccaagcatg ggtctggttg aaaaaccgct
     2461 gatgaacacc accaacgcaa tcctgatcat catgctcagc gttgcaactc tgaccaccgt
     2521 tatctgtaaa gtcgataccg acaacatcct caactccagc accttcaaag caggtatgag
     2581 cgcctgtatt tgtatcctgg gtgttgcgtg gctgggcgat actttcgttt ccaacaacat
     2641 cgactggatc aaagataccg ctggtgaagt gattcagggt catccgtggc tgctggccgt
     2701 catcttcttc tttgcttctg ctctgctgta ctctcaggct gcaaccgcaa aagcactgat
     2761 gccgatggct ctggcactga acgtttctcc gctgaccgct gttgcttctt tcgctgcggt
     2821 gtctggtctg ttcattctgc cgacctaccc gacgctggtt gctgcggtac agatggatga
     2881 cacgggtact acccgtatcg gtaaattcgt cttcaaccat ccgttcttca tcccgggtac
     2941 tctgggtgtt gccctggccg tttgcttcgg cttcgtgctg ggtagcttca tgctgtaatg
     3001 atcaatcgcg gggcgttcac gccccgcttt ctttcccgcc gactaacatc cttcccccgt
     3061 ccgttgtata gtgacctctc tcttgcggtt ccatctgttc ttgcgaggtg tttatgcttg
     3121 atgaaaaaag ttcgaatacc gcgtctgtcg tggtgctatg taccgcaccg gatgaagcga
     3181 cagcccagga tttagccgcc aaagtgctgg cggaaaaact ggcggcctgc gcgaccttga
     3241 tccccggcgc tacttctctc tattactggg aaggtaagct ggagcaagaa tacgaagtgc
     3301 agatgatttt aaaaactacc gtatctcacc agcaggcact gctggaatgc ctgaagtctc
     3361 atcatccata tcaaaccccg gaacttctgg ttttacctgt tacacacgga gacacagatt
     3421 acctctcatg gctcaacgca tctttacgct gatcctgcta ctttgcagca cttccgtttt
     3481 tgccggatta ttcgacgcgc cgggacgttc acaatttgtc cccgcggatc aagcctttgc
     3541 ttttgatttt cagcaaaacc aacatgacct gaatctgacc tggcagatca aagacggtta
     3601 ttacctctac cgtaaacaga tccgcattac gccggaacac gcgaaaattg ccaacgtgca
     3661 gctgccacaa ggcgtctggc atgaagatga gttttacggc aaaagcgaga tttaccgcga
     3721 tcggctgacg cttcccgtaa ccatcaacca ggcgagtgcg ggagcgacgt taactgtcac
     3781 ctaccagggc tgtgctgatg ccggtttctg ttatccgcca gaaaccaaaa ccgttccgtt
     3841 aagcgaagtg gtcgccaaca acgcagcgtc acagcctgtg tctgttccgc agcaagagca
     3901 gcccaccgcg caattgccct tttccgcgct ctgggcgttg ttgatcggta ttggtatcgc
     3961 ctttacgcca tgcgtgctgc caatgtaccc actgatttct ggtatcgtgc tgggtggtaa
     4021 acagcggctc tccactgcca gagcattgtt gctgaccttt atttatgtgc aggggatggc
     4081 actgacttac acggcgctgg gtctggtagt tgccgccgca gggttacagt tccaggcggc
     4141 gctacagcac ccatacgtgc tcattggcct cgccatcgtc tttaccttgc tggcgatgtc
     4201 aatgtttggt ttgtttaccc tgcaactccc ctcttcgctg caaacgcgcc tcacgctgat
     4261 gagcaatcgc caacagggcg gctcacctgg cggtgtgttt gttatgggag cgattgccgg
     4321 actgatctgt tcaccatgca ccaccgcacc gcttagcgcg attctgttgt atatcgccca
     4381 aagcgggaac atgtggctgg ggggcggcac gctttatctc tatgcgttgg gcatgggcct
     4441 gccgctgatg ctaataaccg tctttggtaa ccgcctgctg ccgaaaagcg gcccgtggat
     4501 ggaacaagtc aaaaccgcgt ttggttttgt gatcctcgca ctgccggtct tcctgctgga
     4561 gcgagtgatt ggcgatatat ggggattacg cttgtggtcg gcgcttggtg tcgcattctt
     4621 tggctgggcc tttatcacca gcctacaggc caaacgcggc tggatgcgcg tggtgcaaat
     4681 aatcctgctg gcagcggcat tggttagcgt gcgcccactt caggattggg catttggtgc
     4741 aacacatacc gcgcaaactc agacgcatct caactttaca caaatcaaaa cggtagatga
     4801 gttaaatcag gcgctcgttg aagccaaagg caaaccagtg atgttagatc tctatgccga
     4861 ctggtgcgtc gcctgtaaag agtttgagaa atacaccttc agcgacccgc aggtgcaaaa
     4921 agcgttagca gacacggtct tacttcaggc taacgtaact gccaacgacg cacaagatgt
     4981 ggcgctgtta aagcatctta atgtccttgg cctaccgaca attctctttt ttgacggaca
     5041 aggccaggag catccacaag cacgcgtcac gggctttatg gatgctgaaa ccttcagcgc
     5101 acatttgcgc gatcgccaac cgtgaacgac acttgcagtg ggaaagacgg aggaaaatag
     5161 cgtgcaacgt gaagatgtac tgggagaagc cctgaaatta ttagaattac aagggattgc
     5221 caacaccacg cttgagatgg ttgctgaacg tgtggattac ccactggacg agctacgccg
     5281 cttctggcca gacaaagagg caatcctcta cgatgcgctg cgttatctta gccaacagat
     5341 agatgcctgg cgtcgtcagc tgatgctgga cgaaacgcaa accgccgaac aaaagctgct
     5401 ggcacgttat caggcgttat cggagtgcgt taaaaacaac cgctatccgg gctgtctatt
     5461 tatcgctgcc tgtacgtttt atcccgatcc tggccaccct attcatcaac tggccgatca
     5521 gcaaaaaagc gcggcctacg atttcaccca cgaactgtta accacactgg aagttgacga
     5581 tccggcgatg gtagcaaagc agatggaact ggtgctggaa ggctgtttaa gccgaatgct
     5641 ggtgaatcgt agccaggcgg atgtcgacac cgcacatcgg ctggcggaag atatcctgcg
     5701 cttcgcccgc tgccgccagg gtggtgcttt gacctgacag aaacacagaa aagaagcgat
     5761 ttgccgcaat cttaagcagt tgaatcgctt ttactgaaat taggttgacg agatgtgcag
     5821 attacggttt aatgcgcccc gttgcccgga tagctcagtc ggtagagcag gggattgaaa
     5881 atccccgtgt cggtggttcg attccgcctc cgggcaccat ataccagaac acgctggtta
     5941 agggatgaga tccttgacgg gcggttaata caggatgcgg catcaaaggg tctccgtttg
     6001 tgtaagttgt ggttacactg attgagttcg gtatcgtatc ctcaaacagg gtgtcttttg
     6061 cggagacgcc ctgttctcgt taacactgta tgcttacatc gctgattcag cctgtcaaga
     6121 tttattcccc cctttgcaaa tcttgctcac tgtgcgctca tctgtataca aactcagggc
     6181 atcttccagt gtctg
//
LOCUS       DAZFLD010000232         6159 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.233, whole genome shotgun sequence.
ACCESSION   DAZFLD010000232 DAZFLD010000000
VERSION     DAZFLD010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6159
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.233"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            66..2510
                     /gene="dmsA"
                     /locus_tag="ACHKBL_004213"
     CDS             66..2510
                     /gene="dmsA"
                     /locus_tag="ACHKBL_004213"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009389 - dimethyl sulfoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit A"
                     /protein_id="HFO5820994.1"
                     /translation="MKTKIPDAVLAAEVSRRGLVKTTAIGGLAMASSALTLPFSRIAH
                     AVDSALPAKSDEKVIWSACTVNCGSRCPLRMHVVDGEIKYVETDNTGDDNYDGLHQVR
                     ACLRGRSMRRRVYNPDRLKYPMKRVGARGEGKFERISWEEAYDIIATNMQRLIKEYGN
                     ESIYLNYGTGTLGGTMTRSWPPGNTLVARLMNCCGGYLNHYGDYSSAQIAEGLNYTYG
                     GWADGNSPSDIENSKLVVLFGNNPGETRMSGGGVTYYLEQARQKSNARMIIIDPRYTD
                     TGAGREDEWIPIRPGTDAALVNGLAYVMITENLVDQAFLDKYCVGYDEKTLPASAPKN
                     GHYKAYILGEGPDGVAKTPEWASQITGVPADKIIKLAREIGSTKPAFISQGWGPQRHA
                     NGEIATRAISMLAILTGNVGINGGNSGAREGSYSLPFVRMPTLENPIQTSISMFMWTD
                     AIERGPEMTALRDGVRGKDKLDVPIKMIWNYAGNCLINQHSEINRTHEILQDDKKCEL
                     IVVIDCHMTSSAKYADILLPDCTASEQMDFALDASCGNMSYVIFNDQVIKPRFECKTI
                     YEMTSELAKRLGVEQQFTEGRTQEEWMRHLYAQSREAIPELPTFEEFRKQGIFKKRDP
                     QGHHVAYKAFREDPQANPLTTPSGKIEIYSQALADIAATWELPEGDVIDPLPIYTPGF
                     ESYQDPLNKQYPLQLTGFHYKSRVHSTYGNVDVLKAACRQEMWINPLDAQKRGINNGD
                     KVRIFNDRGEVHIEAKVTPRMMPGVVALGEGAWYDPDTKRVDKGGCINVLTTQRPSPL
                     AKGNPSHTNLVQVEKV"
     gene            2521..3138
                     /gene="dmsB"
                     /locus_tag="ACHKBL_004214"
     CDS             2521..3138
                     /gene="dmsB"
                     /locus_tag="ACHKBL_004214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase iron-sulfur subunit
                     DmsB"
                     /protein_id="HFO5820995.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDYKDLTPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCEDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNETKGHMTKCDGCYDRVAEGKKPICVESCPLRALDFGPIDELRK
                     KHGDLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPKEV"
     gene            3140..4003
                     /gene="dmsC"
                     /locus_tag="ACHKBL_004215"
     CDS             3140..4003
                     /gene="dmsC"
                     /locus_tag="ACHKBL_004215"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit
                     DmsC"
                     /protein_id="HFO5820996.1"
                     /translation="MGSGWHEWPLMIFTVFGQCVAGGFIVLALALLKGDLRAEAQQRV
                     IACMFGLWVLMGIGFIASMLHLGSPMRAFNSLNRVGASALSNEIASGSIFFAVGGIGW
                     LLAMLKKLSPALRTLWLIVTMVLGVIFVWMMVRVYNSIDTVPTWYSIWTPMGFFLTMF
                     MGGPLLGYLLLSLAGVDGWAMRLLPAISVLALVVSGVMSVMQGAELATIHSSVQQAAA
                     LVPDYGALMSWRIVLLAVALCLWIAPQLKGYQPAVPLLSVSFILLLAGELIGRGVFYG
                     LHMTVGMAVAS"
     gene            complement(4039..4665)
                     /gene="ycaC"
                     /locus_tag="ACHKBL_004216"
     CDS             complement(4039..4665)
                     /gene="ycaC"
                     /locus_tag="ACHKBL_004216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate family cysteine hydrolase YcaC"
                     /protein_id="HFO5820997.1"
                     /translation="MTKPYVRLDKNDAAVLLVDHQAGLLSLVRDIEPDKFKNNVLALG
                     DLAKYFNLPTILTTSFETGPNGPLVPELKAQFPDAPYIARPGNINAWDNEDFVKAVKA
                     TGKKQLIIAGVVTEVCVAFPALSAIEEGFDVFVVTDASGTFNEITRHSAWDRMSQAGA
                     QLMTWFGVACELHRDWRNDIEGLATLFSNHIPDYRNLMTSYDTLTKQK"
     gene            4980..6128
                     /locus_tag="ACHKBL_004217"
     CDS             4980..6128
                     /locus_tag="ACHKBL_004217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO5820998.1"
                     /translation="MSTYTRPVMLLLSGLLLLTLAIAVLNTLVPLWLAQEHMSTWQVG
                     VVSSSYFTGNLVGTLLTGYVIKRIGFNRSYYLASFIFAAGCAGLGLMIGFWSWLAWRF
                     VAGVGCAMIWVVVESALMCSGTSRNRGRLLAAYMMVYYVGTFLGQLLVSKVSTELMSV
                     LPWVTGLTLAGILPLLFTRVLNQQAENHDSTSITSMLKLRQARLGVNGCIISGIVLGS
                     LYGLMPLYLNHKGVSNASIGFWMAVLVSAGILGQWPIGRLADKFGRLLVLRVQVFVVI
                     LGSIAMLSQAAMAPALFILGAAGFTLYPVAMAWACEKVEHHQLVAMNQALLLSYTVGS
                     LLGPSFTAMLMQNFSDNLLFIMIASVSFIYLLMLLRNAGHTPKPVAHV"
BASE COUNT         1421 a         1455 c         1695 g         1588 t
ORIGIN      
        1 tagaaataat tacacaatac ggtttgttac tggaatcaat cgtgagcaag cttgagtgag
       61 ccattatgaa aacgaaaatc cctgatgcgg tattggctgc tgaggtgagt cgccgtggtt
      121 tggtaaaaac gacagcgatc ggcggcctgg caatggcaag cagcgcgtta acattacctt
      181 ttagtcggat tgcgcacgct gttgatagtg cccttccggc aaaatcagac gaaaaggtta
      241 tctggagcgc ctgtacagtt aactgtggta gtcgctgccc gctacgtatg cacgttgtgg
      301 acggtgaaat taaatatgtt gaaacggata ataccggcga tgacaactat gacggcctgc
      361 accaggtgcg cgcctgtctg cgtggtcgtt ccatgcgtcg ccgtgtctac aatccggacc
      421 gcctgaaata tccgatgaaa cgagtcgggg cgcgcggtga aggcaaattc gagcgcatta
      481 gctgggaaga agcctacgac atcatcgcga ccaatatgca gcgcctgatc aaagagtacg
      541 gcaacgagtc tatctatctg aactatggta ccggaacgct gggcggcacc atgacccgct
      601 cctggccgcc gggaaatacc ctggtcgctc gcctgatgaa ctgctgcggc ggttatctga
      661 accattacgg tgactactct tccgcgcaaa ttgcggaagg tttgaactat acctacggcg
      721 gctgggcaga tggtaacagc ccgtcggata tcgaaaacag taaactggta gtgctgtttg
      781 gtaataaccc tggcgaaacg cgaatgagtg gcggtggggt gacttactat cttgaacagg
      841 cgcgccagaa atctaatgct cgcatgatca tcatcgatcc gcgctatacc gacaccggtg
      901 ccgggcgcga agatgagtgg atccctattc gtccgggaac agatgccgca ctggttaacg
      961 gtctggcgta tgtcatgatc accgaaaacc tggtggatca ggcattcctc gataaatatt
     1021 gcgttggcta cgacgagaaa acactgccag ccagtgcgcc gaaaaatggt cactataaag
     1081 cttatattct gggtgaaggg ccagatggcg tggctaaaac gccggaatgg gcctcgcaaa
     1141 tcactggtgt tccggcagac aaaatcatca aactggctcg tgaaatcggt agtaccaaac
     1201 cggcgtttat cagccaggga tggggcccgc agcgtcacgc taacggtgaa atcgcaaccc
     1261 gtgctatctc gatgctggcg attctgaccg gtaacgttgg tattaacgga ggcaacagcg
     1321 gcgcgcgtga aggttcatac agcttaccgt ttgtccgtat gccgaccctc gaaaacccga
     1381 tccagaccag catttcgatg tttatgtgga ccgatgccat tgaacgtggc ccggaaatga
     1441 cagcgctgcg tgatggtgtg cgcgggaaag ataagctgga tgtgccgatc aaaatgatct
     1501 ggaactatgc cggtaactgc ctgattaatc agcattctga aatcaaccgt acccatgaaa
     1561 tccttcagga tgataagaag tgcgagctga ttgtggttat cgactgccac atgacctcat
     1621 cggcgaaata tgctgacatc ctgctgcctg actgcaccgc ttcagaacag atggactttg
     1681 cactggatgc atcctgcggg aatatgtctt acgtgatttt caacgatcag gtgattaaac
     1741 cgcgctttga atgtaagacc atctatgaaa tgaccagcga actggcaaaa cgtcttggcg
     1801 ttgagcaaca gtttactgag ggccgtaccc aggaagagtg gatgcggcat ctgtatgccc
     1861 agtcgcggga agcgattcct gaactgccaa cgtttgaaga gttccgtaag caggggatct
     1921 ttaaaaagcg cgacccacaa gggcatcacg ttgcttataa agctttccgt gaagatccgc
     1981 aggcaaatcc actgaccacg ccatcgggga aaattgagat ttattcgcag gcgctggctg
     2041 acattgccgc tacctgggaa ttgccagaag gtgatgtgat cgatccactg ccgatctaca
     2101 cgccgggctt tgaaagttat caggatccgc tgaacaaaca gtatccgctg cagcttacgg
     2161 gtttccacta taaatctcgc gttcactcaa cttacggcaa cgttgatgtg ctgaaagcag
     2221 cttgccgtca ggaaatgtgg atcaacccgc ttgatgcgca aaaacgcggt atcaacaacg
     2281 gcgataaagt caggatcttt aacgatcgtg gtgaggttca tattgaggcg aaagtgacgc
     2341 cacgaatgat gccgggtgtg gtcgcactgg gtgaaggtgc ctggtatgac ccggatacaa
     2401 aacgtgtcga taagggcggt tgtatcaacg tactgaccac tcaacgtccg tctcctctcg
     2461 ctaaggggaa tccgtcacat acaaacctgg ttcaggttga aaaggtgtaa ggagtaaccg
     2521 atgacaaccc agtatggatt ttttattgat tccagccgtt gcaccggttg caaaacctgc
     2581 gagctggctt gtaaagacta caaagatttg acgccagaag tcagtttccg ccgcatttat
     2641 gaatatgctg gcggcgactg gcaggaagat aacggtgtct ggcaccagaa cgtgtttgcc
     2701 tactatctgt cgatttcatg taaccactgc gaagatccgg cttgtactaa agtctgcccg
     2761 agcggtgcga tgcataaacg tgaagatggt tttgttgtgg tcgatgaaga tgtgtgcatt
     2821 ggctgccgtt actgccatat ggcttgcccg tacggcgcac cgcaatataa cgaaacgaaa
     2881 ggccatatga ccaaatgcga tggttgttat gaccgtgttg cggaggggaa aaagccgatc
     2941 tgtgttgaat cttgcccgct gcgggcgctg gatttcggcc ctatcgacga gctgcgtaaa
     3001 aaacatggcg atctggcggc cgttgcgccg ttgccgcgag ctcactttac caaaccgaat
     3061 attgtgatca aacccaatgc caacagccgc ccgaccgggg ataccactgg ctatctggca
     3121 aacccgaagg aggtgtgaga tgggaagtgg atggcatgaa tggccgctga tgatcttcac
     3181 ggtcttcggg caatgtgtag cgggtggttt tatcgttctg gctttggcgc tgctcaaagg
     3241 cgacctgcga gcagaagccc agcagcgtgt tattgcctgc atgtttggtt tatgggtgct
     3301 gatgggcatt ggctttatcg cctctatgct tcatcttggt tcaccgatgc gcgcctttaa
     3361 ctcgctcaac cgggtagggg cttcagcact cagtaacgaa atcgccagcg gttcgatctt
     3421 ttttgctgtt ggtggcatcg gctggttgct ggcaatgctg aaaaagctgt caccggcatt
     3481 gcgtacgctg tggctgatag tgacgatggt tcttggcgtc atctttgtct ggatgatggt
     3541 gcgtgtgtat aacagcattg ataccgttcc gacctggtac agcatctgga cgccgatggg
     3601 cttcttcctg acgatgttca tgggcggccc gttactgggt tacctgttgc tgagtctggc
     3661 tggcgtcgat ggctgggcga tgcgtctgct gccagccatt tctgtactgg cactggtagt
     3721 gagtggcgtg atgtcagtga tgcagggcgc agagctggcg actattcata gctctgtgca
     3781 gcaggccgca gcgctggtgc cggactatgg cgcgctgatg tcctggcgga tcgtgctttt
     3841 ggctgttgcc ctgtgcttgt ggattgcacc acagctaaaa ggttatcagc ctgcggttcc
     3901 gctactttct gtatcattca ttctgcttct ggcaggggaa ttaatcggtc gtggcgtatt
     3961 ctatggtttg catatgaccg tggggatggc tgtcgcaagt taatcagtaa caaccggggt
     4021 ttcggccccg gttcgctttt atttctgctt cgttaacgtg tcataactgg tcatcaagtt
     4081 acgataatca ggaatgtggt tagagaacaa cgtcgccagt ccttcaatat cattacgcca
     4141 gtcgcgatgc agctcgcatg ccacgccgaa ccatgtcatc aattgcgcac cagcttgcga
     4201 catgcgatcc catgccgaat gccgggtaat ttcattaaag gtccctgacg cgtcagtcac
     4261 gacaaagacg tcaaaccctt cttcaatggc tgaaagcgcc gggaatgcca cgcaaacttc
     4321 ggttaccaca ccggcaataa ttaactgttt tttacctgtc gctttgacag cttttacaaa
     4381 atcttcgtta tcccaggcgt taatatttcc cgggcgagca atgtaaggtg catcggggaa
     4441 ttgtgctttc agttctggaa ctaatgggcc gttaggaccg gtttcaaaac tggtggtaag
     4501 aatagttggt aagttgaaat acttagccag gtcacccagc gccagcacgt tatttttaaa
     4561 cttatcgggt tcgatatccc gtacaaggga aagtaaacca gcctggtgat caacaagcaa
     4621 aacggcagca tcatttttat caagacgaac atacggtttg gtcatcctct tctcctttcg
     4681 agagtcagtg gtacgtctga aatcggtcag acgcgatgca ttgctctgaa agcatagacg
     4741 ggaaatatga gtttgctgtg accatgaaat ttttcgactg aacgaagcgt atgaaatttg
     4801 tgttagatca ataaaaacaa tcagatgaga ttaatcgtct ctccagcatt agcgttatga
     4861 atgagtaata taaatcgata atacctccgc tgcgatatgc gcgtattttc acccattgac
     4921 aatgtttttg gcggtggcat gatgcgcatg aaatttgaac ttcctcacgg ttttaattca
     4981 tgtccacgta tacccggcct gtcatgcttt tgctgtctgg cctgcttttg ttgactctgg
     5041 cgatcgcagt gttaaataca ctcgtgccgc tttggctcgc ccaggaacac atgtccacat
     5101 ggcaggtagg cgttgtcagc tcatcctatt ttaccggcaa cctggtcggt acattgctga
     5161 cagggtatgt cattaagcgc attggcttta accgcagcta ttatctggcc tccttcattt
     5221 ttgccgctgg ctgtgccggc cttggcctga tgattggatt ctggagctgg ttggcttggc
     5281 gttttgtcgc cggtgtcggt tgtgccatga tttgggtggt tgttgagagc gcgctgatgt
     5341 gcagtgggac gtcacgtaac cgtgggcgtt tgcttgctgc gtatatgatg gtttattacg
     5401 tgggaacgtt tttaggccag ttactggtca gcaaagtttc aaccgagctg atgtccgtat
     5461 tgccgtgggt tacaggtttg acgttggcag ggatcttacc gctgttgttt acgcgtgtgc
     5521 tgaatcagca ggctgaaaac catgattcga cgtcaattac gtcaatgcta aaactccgtc
     5581 aggcgcggct tggcgtgaat ggctgcatta tctcaggaat cgtgctggga tctctatatg
     5641 gcctgatgcc gctgtacctc aatcacaaag gggtgagcaa tgccagcatt ggtttctgga
     5701 tggcggtact ggtcagtgcg ggtatcctcg gacaatggcc gatcggacgt ctggcggata
     5761 agtttggtcg actgctggtg ttgcgtgttc aggtctttgt cgtcattctc ggcagtatcg
     5821 cgatgcttag ccaggcggcg atggccccag cgttattcat cctcggtgcc gctggcttta
     5881 cgctatatcc ggtggcgatg gcctgggctt gtgagaaagt tgaacatcat caactggtgg
     5941 cgatgaacca ggccttactg ttgagctata ccgtgggaag tctgcttggc ccgtcattta
     6001 ccgctatgct aatgcagaat ttctccgata atttattgtt tatcatgatc gccagcgtat
     6061 cgtttatcta tttgctgatg ctgcttcgca acgccggtca tacgccgaaa cccgttgctc
     6121 acgtgtaaat gaattcaagc agagtgtgaa cttactgtt
//
LOCUS       DAZFLD010000233         6117 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.234, whole genome shotgun sequence.
ACCESSION   DAZFLD010000233 DAZFLD010000000
VERSION     DAZFLD010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6117)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6117)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6117
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.234"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..184
                     /locus_tag="ACHKBL_004218"
     CDS             <1..184
                     /locus_tag="ACHKBL_004218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001590091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5820999.1"
                     /translation="LTINYGTETSPELSGISYEDANALITELILFIEDVDAENHEAYQ
                     GHLNEITRILNFAIAA"
     gene            complement(236..826)
                     /gene="cobO"
                     /locus_tag="ACHKBL_004219"
     CDS             complement(236..826)
                     /gene="cobO"
                     /locus_tag="ACHKBL_004219"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HFO5821000.1"
                     /translation="MSDERYQQRQQRVKEKVDARVAQAQDERGIIIVFTGNGKGKTTA
                     AFGTATRAVGHGKKVGIVQFIKGTWPNGERNLLEPHGVEFQVMATGFTWDTQNRESDT
                     AACREVWQHAKRMLADPSLDMVLLDELTYMVAYDYLPLEEVVQALNERPHQQTVIITG
                     RGCHRDILELADTVSELRPVKHAFDAGVKAQIGIDY"
     gene            complement(823..1581)
                     /locus_tag="ACHKBL_004220"
     CDS             complement(823..1581)
                     /locus_tag="ACHKBL_004220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HFO5821001.1"
                     /translation="MHYQPKQDLLNDRIILVTGASDGIGREAAMTYARYGATVILLGR
                     NEEKLRQVASHINEETGRQPQWFILDLLTCTSEDCQQLAQRIAVNYPRLDGVLHNAGL
                     LGDVCPMSEQNPQVWQDVMQVNVNATFMLTQALLPLLLKSDAGSLVFTSSSVGRQGRA
                     NWGAYAASKFATEGMMQVLADEYQQRLRVNCINPGGTRTAMRASAFPTEDPQKLKTPA
                     DIMPLYLWLMGDDSRRKTGMTFDAQPGRKPGISQ"
     gene            1801..2850
                     /gene="sohB"
                     /locus_tag="ACHKBL_004221"
     CDS             1801..2850
                     /gene="sohB"
                     /locus_tag="ACHKBL_004221"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HFO5821002.1"
                     /translation="MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRV
                     NNLSEQYKEMKEELAAALMDSHQQKQWHKAQKKKHKQEAKAAKAKAKLGEVATDSKPR
                     VWVLDFKGSMDAHEVNSLREEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQR
                     LRDKNIPLTVTVDKVAASGGYMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKD
                     IDIELHTAGQYKRTLTLLGENTEEGREKFREELNETHQLFKDFVKRMRPSLDIEQVAT
                     GEHWYGQQAVEKGLVDEINTSDEVILSLMEGREVVNVRYMQRKRLIDRFTGSAAESAD
                     RLLLRWWQRGQKPLM"
     gene            complement(2886..3137)
                     /locus_tag="ACHKBL_004222"
     CDS             complement(2886..3137)
                     /locus_tag="ACHKBL_004222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciN family protein"
                     /protein_id="HFO5821003.1"
                     /translation="MNKETQPIDRETLLKEANKIIREHEDTLAGIEATGVTQRNGVLV
                     FTGDYFLDEQGLPTAKSTAVFNMFKHLAHVLSEKYHLVD"
     gene            3517..6114
                     /gene="topA"
                     /locus_tag="ACHKBL_004223"
     CDS             3517..6114
                     /gene="topA"
                     /locus_tag="ACHKBL_004223"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HFO5821004.1"
                     /translation="MGKALVIVESPAKAKTINKYLGSDYVVKSSVGHIRDLPTSGSAA
                     KKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEK
                     ADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNID
                     RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEE
                     FWEVDASTTTPSGEALALQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPT
                     TSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNM
                     VRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQ
                     LIWRQFVACQMTPAKYDSTTLTVGAGDFRLKARGRILRFDGWTKVMPALRKGDEDRIL
                     PAVDKGDALTLVELTPAQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRG
                     YVRVENRRFYAEKMGEIVTDRLEENFRELMNYDFTAQMENSLDQVANHEAEWKAVLDH
                     FFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYAL
                     PPKERCKTTINLVPENEVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHV
                     CGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHLKMGRFGKYMACTNEECKNTR
                     KILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVE
                     ELYRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYVSSEKDGKATGWSAFYV
                     DGKWVEGKK"
BASE COUNT         1576 a         1460 c         1629 g         1452 t
ORIGIN      
        1 tctgacgatt aactacggta ctgaaacttc gccagagtta agcggaatca gttatgaaga
       61 tgccaatgcg ttaattactg aactgatatt atttattgaa gatgttgacg ctgagaatca
      121 tgaagcatat cagggacatt tgaatgagat tacacgtatt ctgaatttcg ctattgccgc
      181 ttgaatataa tcatgattaa agcacctcaa gaggcttctt ttgaggtgct ttcacttagt
      241 agtcaatccc tatctgcgct tttacaccgg catcaaacgc atgtttcacg gggcgtaatt
      301 cactcaccgt atccgccagt tcaagaatat cccgatgaca gcctcgaccc gtgataatca
      361 ccgtctgttg atgtggacgt tcatttaacg cctgcaccac ttcttccagt ggcaaatagt
      421 catacgccac catatacgtc agttcatcga gcaaaaccat atccagtgaa ggatcggcca
      481 gcattcgttt ggcatgttgc cagacttcac ggcaggccgc ggtatcagat tcgcgatttt
      541 gtgtatccca ggtaaagccc gttgccatca cctgaaactc aacgccatgt ggctccagta
      601 gattgcgctc gccattaggc caggtgcctt taataaactg cacgataccc acttttttgc
      661 catggcctac tgcacgagtt gccgtaccaa atgccgcggt ggttttgcct tttccattgc
      721 cggtaaagac gatgataata ccgcgctcat cctgggcctg ggccacacga gcatcgactt
      781 tttctttcac tcgctgctga cgctgttggt agcgttcatc actcattggg aaattcctgg
      841 tttacggccc ggttgggcgt caaaggtcat gccggtttta cggcggctgt catcgcccat
      901 cagccagagg tagagcggca tgatatcagc gggtgtttta agtttctgtg gatcttcggt
      961 cgggaaggcg ctggcacgca ttgcggtgcg cgtaccgcct gggttaatgc agttgacgcg
     1021 caggcgctgc tgatattcat cggccagtac ctgcatcatc ccttcggtgg caaatttcga
     1081 cgctgcatat gcgccccagt tggctcgtcc ctgacgtcca acgcttgatg aagtaaagac
     1141 cagtgaaccg gcgtccgatt tgagtaataa aggaagcagt gcctgggtga gcataaaggt
     1201 ggcattaacg ttgacctgca tgacgtcctg ccagacctgc ggattttgtt cgctcattgg
     1261 gcaaacatcg ccgagcaatc cggcattatg caaaacacca tccagacgcg gataattaac
     1321 ggcaatgcgc tgtgccagtt gttggcaatc ttcggacgtg caggtcagca aatcgagaat
     1381 aaaccactgt ggctgacgtc cagtttcttc gtttatgtgg ctggctacct gacgtaattt
     1441 ttcttcatta cggcccaaca gaatcactgt cgcaccatag cgtgcatacg tcatcgcggc
     1501 ttcacggcca ataccatcgc tggctcccgt caccaggata atgcgatcat tgagtaaatc
     1561 ttgttttggc tggtaatgca tggctactcc tcaacgacgt tgtctgtcgc gctgccagtg
     1621 tactttatga ctttggggct ttatgcctga aacagaaggg catttcaatc agcctggggc
     1681 gataacaccg caaaatggat actttgtcat actttcgctg caataacatc tctgcgagac
     1741 ggcttaacat gcctgttgta aactgtgagc caaagcgttg tttaaccaag gtggggactc
     1801 gtggaattgt tgtctgaata tggtttgttt ttggcgaaaa tcgttaccgt tgtgctagcg
     1861 attgcggcga ttgccgccat tattgtcaat gttgctcaac gtaataaacg ccagcgtggc
     1921 gagttacggg tcaacaatct cagcgaacag tataaggaga tgaaagaaga actggccgcg
     1981 gcgctgatgg actcacatca gcaaaaacag tggcacaaag cgcagaagaa aaagcacaag
     2041 caagaagcga aagcagcaaa agcgaaagcc aaactgggcg aggtggcaac tgacagtaaa
     2101 cctcgcgtct gggtgctgga ttttaaaggc agcatggacg cccatgaagt gaactcgcta
     2161 cgtgaagaga taacggcggt actcgcagca ttcaaaccgc aggatcaggt tgtgctccgt
     2221 ctggaaagcc ctggtggcat ggtgcatggt tacgggttgg cggcttcgca gctgcagcgt
     2281 ctgcgtgata aaaacattcc tttaactgta acggtagaca aagtcgctgc cagcggcggt
     2341 tacatgatgg cctgtgtggc ggacaaaatt gtttccgcac cgtttgctat tgtgggttcc
     2401 attggcgtgg tggcgcaaat gcccaacttt aaccgcttcc tgaaaagcaa agatattgat
     2461 atcgaactgc acaccgccgg gcagtataag cgcacgctga ccttgctggg tgaaaacacc
     2521 gaagaagggc gggagaaatt ccgcgaagag ctgaacgaaa cgcatcagtt atttaaagat
     2581 tttgtgaagc gtatgcgtcc gtctctggat attgaacagg tggcaacggg tgaacactgg
     2641 tacggacaac aggcggtaga gaaaggcctg gttgatgaaa tcaacaccag tgatgaagtt
     2701 attcttagcc tgatggaagg ccgtgaagtg gtaaatgtac gctatatgca gcgtaaacga
     2761 ctcattgacc gattcaccgg cagcgcggca gagagcgccg atcgattgtt gctacgctgg
     2821 tggcagcggg gtcaaaagcc attgatgtaa aagacaaacg cgagggtaag acctcgcgtt
     2881 ttgctttaat caaccaggtg atatttttct gaaagcacat gggccaggtg tttgaacata
     2941 ttaaacaccg cggtgctttt ggctgttggc aatccttgtt catctaaaaa gtagtcgccg
     3001 gtaaatacca gcacgccatt acgctgcgtg acgccggtgg cttcaatccc tgcgagcgta
     3061 tcctcatgct cacgaatgat tttgttggcc tctttcaaca gcgtctcgcg gtcgatgggt
     3121 tgtgtctctt tgttcattat ttactcctta aacaaggaca ttagtctacg ccaggcatgg
     3181 cttgcagaca aatataccac gctggtggca agagcgcctt actggcaact ttggattttg
     3241 catgctaata aagttgcgta tcggatttta tcaggtacag tgtgacgctt tcgtcaatct
     3301 ggcaatagat ttgcttgaca ttcgaccaaa attccgtcgt gctatagcgc ctgtaggcca
     3361 agacctgtta actcagtcac ctgaattttc gtgaacagag tcacgacaag gggttgatat
     3421 ccgcagagag cgagtccata tcggtaactc gttgccagtg gaaggtttat caacgtgcga
     3481 cgcattcctg gaagaatcaa attaggtaag gtgaatatgg gtaaagctct tgtcatcgtt
     3541 gagtccccgg caaaagccaa aacgatcaac aagtatctgg gtagtgacta cgtggtgaaa
     3601 tccagcgtcg gtcacatccg cgatttgccg accagtggct cagctgccaa aaagagtgcc
     3661 gactctacct ccaccaagac ggctaaaaag cctaaaaagg atgaacgtgg cgctctcgtc
     3721 aaccgtatgg gggttgaccc gtggcacaat tgggaggcgc actatgaagt gttgcctggt
     3781 aaagagaagg tcgtctctga actgaaacaa ctggctgaaa aagccgacca catctatctc
     3841 gcaaccgacc ttgaccgcga aggggaagcc attgcatggc acctgcggga agtgattggt
     3901 ggtgatgatg cgcgctatag ccgagtggtg tttaacgaaa ttactaaaaa cgcgatccgc
     3961 caggcattta acaaaccggg tgagctgaat attgatcgtg ttaatgccca gcaggcgcgt
     4021 cgctttatgg accgcgtggt ggggtatatg gtttcgccgc ttctatggaa aaagatcgct
     4081 cgtggtctgt ctgccggtcg tgtgcagtcg gtggcggttc gcctggtggt cgagcgtgag
     4141 cgtgaaatta aagcgttcgt accggaagag ttctgggaag tcgatgccag cacgaccacg
     4201 ccatctggtg aagcgttggc gttgcaggtg actcatcaga acgacaaacc gttccgtccg
     4261 gtcaacaaag aacaaactca ggctgcggta agtctgctgg aaaaagcgcg ctacagcgtg
     4321 ctggaacgtg aagacaaacc gacaaccagt aaacctggcg ctccttttat tacctctacg
     4381 ctgcaacaag ctgccagcac ccgtcttgga tttggcgtga aaaaaaccat gatgatggcg
     4441 cagcgtttgt atgaagcagg ctatatcact tacatgcgta ccgactccac taacctgagt
     4501 caggacgcgg taaatatggt tcgcggttat atcagcgata attttggtaa gaaatatctg
     4561 ccggaaagtc cgaatcagta cgccagcaaa gaaaactcac aggaagcgca cgaagcgatt
     4621 cgtccttctg acgtcaatgt gatggcggaa tcgttgaagg atatggaagc agatgcgcaa
     4681 aaattgtacc agttaatctg gcgtcagttc gttgcctgcc agatgacccc agcgaaatat
     4741 gactccacga cgctgaccgt tggtgcgggc gatttccgcc tgaaagcacg cggtcgtatt
     4801 ttgcgctttg atggctggac aaaagtgatg cctgcactgc gtaaaggcga tgaagatcgt
     4861 atcttaccgg cagtcgataa aggcgatgct ctgacgctcg ttgaactgac accagcccag
     4921 cactttacca aaccgccagc ccgtttcagt gaagcatcgc tggttaaaga actggaaaaa
     4981 cgcggtatcg gtcgtccgtc tacctatgcg tcgatcattt cgaccattca ggatcgtggc
     5041 tatgtgcgtg tagaaaaccg tcgtttctat gcggaaaaaa tgggcgaaat cgtcaccgat
     5101 cgccttgaag aaaatttccg cgagttaatg aactacgact ttaccgcgca gatggaaaac
     5161 agccttgacc aggtggcaaa tcacgaagca gagtggaaag ctgtactgga tcacttcttc
     5221 tcggatttca cccagcagtt agataaagct gaaaaagatc cggaagaggg ggggatgcgc
     5281 ccgaaccaga tggttctgac cagcatcgac tgcccgacct gtggtcgcaa aatggggatt
     5341 cgcacagcga gcaccggggt attccttggc tgttccggct acgctctgcc accgaaagag
     5401 cgttgcaaaa ccaccatcaa cctggtgccg gaaaacgaag tgctgaacgt gctggaaggc
     5461 gaagatgctg aaaccaacgc gctgcgcgca aaacgtcgtt gcccgaaatg cggcacggcg
     5521 atggacagct atctcatcga tccgaaacgt aagttgcatg tctgtggtaa taacccaacc
     5581 tgcgacggtt acgagatcga agagggcgaa ttccgcatta aaggttatga cggcccgatc
     5641 gttgagtgtg aaaaatgtgg ctctgaaatg cacctgaaaa tggggcgttt cggtaaatac
     5701 atggcctgca ctaacgaaga gtgtaaaaac acgcgtaaga ttttacgtaa cggcgaagtg
     5761 gcaccaccga aagaagatcc ggtgccatta ccggagctgc cgtgcgaaaa atcagatgct
     5821 tatttcgtgc tgcgtgacgg tgctgccggt gtgttcctgg cggccaatac cttcccgaaa
     5881 tcgcgtgaaa cacgtgcgcc actggtggaa gagctttatc gcttccgcga ccgtctgccg
     5941 gaaaaactgc gttatctggc cgatgcgcca cagcaggatc cggaaggtaa taagaccatg
     6001 gttcgcttta gccgtaaaac caaacagcaa tatgtctctt cggaaaaaga cggaaaggcg
     6061 actggctggt cagcatttta tgttgatggc aaatgggttg aagggaaaaa ataacct
//
LOCUS       DAZFLD010000234         6096 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.235, whole genome shotgun sequence.
ACCESSION   DAZFLD010000234 DAZFLD010000000
VERSION     DAZFLD010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6096
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.235"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            242..1372
                     /gene="ompC"
                     /locus_tag="ACHKBL_004224"
     CDS             242..1372
                     /gene="ompC"
                     /locus_tag="ACHKBL_004224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HFO5821005.1"
                     /translation="MKVKVLSLLVPALLVAGAANAAEVYNKDGNKLDLYGKVDGLHYF
                     SDDKSVDGDQTYMRLGFKGETQVTDQLTGYGQWEYQIQGNSAENENNSWTRVAFAGLK
                     FQDVGSFDYGRNYGVVYDVTSWTDVLPEFGGDTYGSDNFMQQRGNGFATYRNTDFFGL
                     VDGLNFAVQYQGKNGSVDGEGMTNNGRGALRQNGDGVGGSITYDYEGFGIGAAVSSSK
                     RTDAQNGTYVSDNNVVRNYIGTGDRAETYTGGLKYDANNIYLAAQYTQTYNATRVGSL
                     GWANKAQNFEAVAQYQFDFGLRPSLAYLQSKGKNLGVINGRNYDDEDILKYVDVGATY
                     YFNKNMSTYVDYKINLLDDNQFTRDAGINTDNIVALGLVYQF"
     gene            1484..2539
                     /gene="apbE"
                     /locus_tag="ACHKBL_004225"
     CDS             1484..2539
                     /gene="apbE"
                     /locus_tag="ACHKBL_004225"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase ApbE"
                     /protein_id="HFO5821006.1"
                     /translation="MEISFTRVALLAAALFFVGCDQQPQPAKTHATEVTVLEGKTMGT
                     FWRASIPGIDAKRSAELKEKIQTQLDADDQLLSTYKKDSALMRFNDSQSLSPWPVSEA
                     MADIVTTSLRIGAKTDGAMDITVGPLVNLWGFGPEQQPVQIPSQEQIDAMKAKTGLQH
                     LTVINQSHQQYLQKDLPDLYVDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSR
                     GMNGAGKPWRVAIQKPTDKENAVQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQ
                     TGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPEKAKEVVRREGLAVYMITKEGDNF
                     KTWMSPQFKSFLISEKN"
     gene            2613..3677
                     /gene="ada"
                     /locus_tag="ACHKBL_004226"
     CDS             2613..3677
                     /gene="ada"
                     /locus_tag="ACHKBL_004226"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HFO5821007.1"
                     /translation="MKYATCLTDDQRWQSVLARDSNADGEFVFAVRTTGIFCRPSCRA
                     RHALRENVSFYANASEALAAGFRPCKRCQPDKANPLQHRLDKITHACRLLEQETPVTL
                     EALADQVAMSPFHLHRLFKATTGMTPKAWQQAWRARRLRELLAKGESVTTSIINAGFP
                     DSSSYYRKADETLGMTAKQFRHGGENLAVRYALADCELGRCLVAESERGICAILLGDD
                     DATLISELQQMFPAADSAPADLTFQQHVRDVIASLNQRDTPLTLPLDIRGTAFQQQVW
                     QALRTIPCGETVSYQQLANAIGKPKAVRAVASACAANKLAIVIPCHRVVRGDGTLSGY
                     RWGVSRKAQLLRREAENEER"
     gene            3677..4327
                     /gene="alkB"
                     /locus_tag="ACHKBL_004227"
     CDS             3677..4327
                     /gene="alkB"
                     /locus_tag="ACHKBL_004227"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HFO5821008.1"
                     /translation="MLDLFADAEPWQEPLAAGAVILHRFAFNAAEQLIRDINDVASQS
                     PFRQMVTPGGYTMSVAMTNCGHLGWTTHRQGYLYSPIDPQTNKPWPAMPQSFHHLCQR
                     AATAAGYPDFQPDACLINRYAPGAKLSLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLK
                     RNDPLKRLLLEHGDVVVWGGESRLFYHGIQPLKAGFHPLTTDCRYNLTFRQAGKKE"
     gene            4403..6046
                     /gene="yojI"
                     /locus_tag="ACHKBL_004228"
     CDS             4403..6046
                     /gene="yojI"
                     /locus_tag="ACHKBL_004228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin J25 efflux ABC transporter YojI"
                     /protein_id="HFO5821009.1"
                     /translation="MELLVLVWRQYRWPFISVMALSLASAALGIGLIAFINQRLIETA
                     DTSLLVLPEFLGLLLLLMAVTLGSQLALTTLGHHFVYRLRSEFIKRILDTHVERIEQL
                     GSASLLAGLTSDVRNITIAFVRLPELVQGIILTIGSAAYLWMLSGKMLLVTAIWMAVT
                     IWGGFVLVARVYKHMATLRETEDKLYTDFQTVLEGRKELTLNRERAEYVFNNLYIPDA
                     QEYRHHIIRADTFHLSAVNWSNIMMLGAIGLVFWMANSLGWADTNVAATYSLTLLFLR
                     TPLLSAVGALPTLLTAQVAFNKLNKFALAPFKAEFPRPQAFPNWQTLELRNVTFSYQD
                     NAFSVGPINLTIKRGELLFLIGGNGSGKSTLAMLLTGLYQPQNGEILLDGKPVSGEQP
                     EDYRKLFSAVFTDVWLFDQLLGPEGQPANPQLVEKWLAQLKMAHKLELSNGRIVNLKL
                     SKGQKKRVALLLALAEERDIILLDEWAADQDPHFRREFYQVLLPLMQEMGKTIFAISH
                     DDHYFIHADRLLEMRNGQLSELTGEERDAASRDAVARTA"
BASE COUNT         1448 a         1531 c         1643 g         1474 t
ORIGIN      
        1 tattgcttga tgttaggtgc ttatttggtc attccgcaat aatcttaaaa agttcccttt
       61 catttacatt ctgaaacatc tatagcgata aatgaaacat cttaaaagtt ttagtatcat
      121 attcgtgttg gattattctg catttttggg gagaatggac ttgccgactg attaatgagg
      181 gttaatcagt atgcagtggc ataaaaaagc aaataaaggc atataacaga gggttaataa
      241 catgaaagtt aaagtactgt ccctcctggt cccagctctg ctggtagcag gcgcagcaaa
      301 cgctgctgaa gtttacaaca aagacggcaa caaattagat ctgtacggta aagtagacgg
      361 cctgcactat ttctctgacg acaagtctgt agatggcgac cagacctaca tgcgtcttgg
      421 cttcaaaggt gaaactcagg ttactgacca actgaccggt tacggccagt gggaatatca
      481 gatccagggc aacagcgctg aaaacgaaaa caactcctgg acccgtgtgg cattcgcagg
      541 tctgaaattc caggatgtgg gttctttcga ctacggtcgt aactacggcg ttgtttatga
      601 cgtaacttcc tggaccgacg tcctgccaga attcggtggc gacacttacg gttctgacaa
      661 cttcatgcag cagcgtggta acggcttcgc gacctaccgt aacaccgact tcttcggtct
      721 ggttgacggc ctgaactttg ctgttcagta ccagggtaaa aacggtagcg tagatggcga
      781 aggcatgacc aacaacggtc gtggtgctct gcgtcagaac ggcgacggcg ttggcggttc
      841 tatcacttat gattacgaag gcttcggtat cggtgctgca gtgtccagct ccaaacgtac
      901 tgacgctcag aatggtacct atgtaagcga caataatgtg gtccgtaact acatcggtac
      961 tggtgaccgt gctgaaacct acactggtgg tctgaaatac gacgctaaca acatctacct
     1021 ggctgctcag tacacccaga cctacaacgc aactcgcgta ggttccctgg gttgggcgaa
     1081 caaagcacag aacttcgaag ctgttgctca gtaccagttc gacttcggtc tgcgtccgtc
     1141 cctggcttac ctgcagtcta aaggtaaaaa cctgggtgtc atcaatggtc gtaactacga
     1201 cgacgaagat atcctgaaat atgttgatgt tggtgcgacc tactacttca acaaaaacat
     1261 gtccacctat gttgactaca aaatcaacct gctggacgac aaccagttca ctcgtgacgc
     1321 tggcatcaac actgataaca tcgtagctct gggtctggtt taccagttct aatctcgatt
     1381 gatatcgaac aaagggcctg cgggcccttt tttcattgtt ttcagcgtac aaacacagtt
     1441 ttttggtgta ctcttgcgac cgttcgcatg aggataatca cgtatggaaa taagctttac
     1501 ccgcgtggca ctgctggctg ccgcgctctt ctttgttggt tgcgatcaac aaccacaacc
     1561 cgccaaaacc cacgctactg aagttaccgt tcttgaaggc aaaaccatgg gtaccttctg
     1621 gcgtgccagc atcccgggca ttgacgccaa acgcagtgcc gaacttaaag aaaagattca
     1681 gacccaactg gacgctgacg atcagctgct ttcgacctat aaaaaagatt ccgcgctgat
     1741 gcgctttaac gactcgcaaa gtttgtcgcc gtggccggta agtgaagcga tggccgatat
     1801 cgtcactacc tcgctgcgca ttggcgcgaa gaccgatggc gcgatggata taaccgtcgg
     1861 gccgctggtg aatctgtggg gctttggtcc ggaacaacaa ccggttcaaa ttccgagcca
     1921 ggaacagatc gatgcgatga aagccaaaac cggcttacag cacctgacgg tcattaatca
     1981 gtcgcatcag caatatctgc aaaaagacct gccggattta tatgtcgatc tctccaccgt
     2041 cggcgaaggt tatgcggcgg atcacctggc acgcttgatg gagcaggaag ggatttcccg
     2101 ctatctggtg tcggtgggcg gcgcgctgaa cagccgtggt atgaacggtg ctggcaagcc
     2161 gtggcgggta gcgattcaaa agccaaccga taaagaaaac gcggttcagg ccgtggtgga
     2221 tatcaacggt catgggatta gtacctctgg cagctaccgt aactattacg aactggacgg
     2281 caaacgtctt tctcatgtta tcgatccaca aaccgggcgt cctatcgaac acaatctggt
     2341 atccgtgacg gtgattgccc cgacggctct ggaagccgat gcctgggata ctggcttaat
     2401 ggtactcgga ccggagaaag ccaaagaggt tgttcgccgg gaaggactgg cggtctacat
     2461 gatcaccaaa gagggcgata attttaaaac ctggatgtcg ccgcagttta aaagcttcct
     2521 tatcagcgaa aaaaattaaa gcgcaagatt gttggttttt gtgcgatggt gaccgggcag
     2581 actaaaggct atccttaacc agggagctga ttatgaaata tgccacatgc ttaactgacg
     2641 atcaacgctg gcaatctgtc ttagcccgcg actcgaatgc cgacggcgaa ttcgttttcg
     2701 ccgtgcgcac cacgggtatc ttttgccgtc cgtcttgccg agccagacat gccttgcggg
     2761 aaaatgtctc cttctacgca aatgccagcg aggcgcttgc cgctggcttt cgtccctgca
     2821 aacgttgtca gccagacaaa gccaatcccc tgcaacatcg ccttgataaa attacccacg
     2881 cctgccgact tctggaacag gaaacgcctg taacgctgga agccttagcc gaccaggtgg
     2941 cgatgagtcc atttcatttg catcggttgt ttaaagcgac taccggaatg acgcctaaag
     3001 cctggcaaca ggcctggcgc gctcgccgtt tgcgcgaatt gctggcgaaa ggggagagcg
     3061 tgactacatc tattattaac gccggattcc ccgacagcag cagttactac cgcaaagccg
     3121 acgaaacgct gggcatgacg gctaaacaat tccgtcacgg tggcgaaaat ctggcggtgc
     3181 gttacgcgct ggctgattgt gagctgggcc gttgcctggt ggcagaaagt gagcggggga
     3241 tttgcgcgat attactgggc gatgatgacg cgacactaat cagcgagtta cagcagatgt
     3301 ttcccgctgc cgacagcgcg cctgctgatc ttacgtttca gcaacatgtg cgtgacgtga
     3361 tcgccagcct caatcaacgc gatacgccgc tgacgttacc gctggacatt cgcggcactg
     3421 cttttcagca acaagtctgg caggcactgc gcacgatacc ttgcggtgaa accgtcagtt
     3481 atcagcaact ggctaatgcc atcggcaaac cgaaagcggt acgggccgtt gccagcgcct
     3541 gtgccgccaa caagctggct atcgtaatac cctgtcatcg ggtggtccgt ggtgatggca
     3601 cactttccgg ttaccgctgg ggcgtgtcgc gcaaagcgca actgctgcgc cgcgaagctg
     3661 aaaatgagga aaggtaatgt tggatctgtt tgccgatgct gaaccgtggc aagaaccact
     3721 ggcggctgga gcggtgattt tgcaccgttt tgcttttaac gctgcggagc aactgatccg
     3781 cgatattaat gacgttgcca gccagtcgcc gtttcgccag atggtcactc caggcggata
     3841 taccatgtcg gtggcgatga ccaactgtgg gcatctgggc tggacgaccc atcggcaagg
     3901 ttatctctat tcgcccattg atccgcaaac aaataaaccg tggcccgcca tgccgcagag
     3961 ttttcatcat ttatgtcaac gtgcggctac ggcggcgggc tatccagatt tccagccaga
     4021 tgcctgtttg atcaaccgct acgctcctgg cgcgaaactg tcgctgcatc aggataaaga
     4081 cgaaccggat ctgcgcgcgc cgattgtttc tgtttctctg ggcttacccg cgatttttca
     4141 atttggcggc ctgaaacgaa atgatccgct aaaaagattg ttgctggaac atggcgatgt
     4201 ggtggtatgg ggcggtgaat cgcggctgtt ttatcacggt attcaaccgt tgaaagcggg
     4261 gtttcatcca ctcaccaccg actgccgcta caacctgaca ttccgtcagg caggtaaaaa
     4321 agaataaaaa taagaattat tattgctgtg cgcgcgaaga tgtttaaact gcgggctgta
     4381 attcattgtc cgggttttct gcatggaact tcttgtactt gtctggcggc agtatcgctg
     4441 gccatttatc agtgtgatgg cgctaagcct cgccagtgcg gcattaggca ttggcttaat
     4501 tgcttttatc aatcagcgcc ttatcgaaac ggcggatacc agtctgctgg tattgccgga
     4561 gtttctgggg ttattgctgc tgttgatggc cgtcactctc ggatcgcaac tggcgctcac
     4621 cactttgggg catcacttcg tttaccgact gcgtagtgaa tttatcaagc ggattctcga
     4681 tactcacgtc gagcgcattg aacaactcgg tagcgcctcg ttgctggcgg gattaaccag
     4741 cgatgtgcgc aatatcacca ttgcttttgt gcgtctgccg gaactggtgc aggggatcat
     4801 tctcactatc ggttcagcgg cgtatttgtg gatgctgtcg ggcaaaatgt tgctggtaac
     4861 ggctatctgg atggcagtca ccatctgggg cggttttgtg ctggtggcgc gggtgtacaa
     4921 acatatggcg accctgcgtg aaaccgaaga taagctgtac acggattttc aaactgtact
     4981 cgaagggcgc aaagagctga ctctgaaccg tgaacgcgcc gagtatgtgt ttaacaacct
     5041 ctacattcct gatgcgcaag agtatcgcca tcatattatt cgcgcagaca ccttccatct
     5101 tagtgccgtg aactggtcaa acatcatgat gctgggcgct atcggcctgg tgttctggat
     5161 ggcgaacagt ctcggttggg ctgataccaa cgttgctgcg acctattcgt tgacgctgtt
     5221 attcctgcgt acgccgctgc tttcggcggt tggcgcattg ccgacgctgc tgacggcgca
     5281 ggtggcgttt aacaagctga acaaattcgc gctcgcgcct ttcaaagcag agtttccgcg
     5341 cccgcaggct tttcccaact ggcaaacgct ggagctgcgt aacgtgacgt ttagctatca
     5401 ggataacgcg ttttccgttg ggccaattaa cctcaccatc aaacgtggcg agctgctgtt
     5461 tctgattggt ggcaacggta gtggaaaatc gacgctggcg atgttgctga cgggcttgta
     5521 ccagccacaa aatggcgaaa tcttgctgga tggcaaacct gtcagcggcg aacaaccgga
     5581 agattatcga aaactgtttt cggcagtgtt taccgatgtc tggctgtttg atcaactgct
     5641 ggggccggag gggcaacccg ctaacccgca actggttgag aagtggctgg cgcagctgaa
     5701 aatggctcat aagcttgagt taagcaacgg gcgtattgtt aacctgaagt tatcaaaagg
     5761 gcagaaaaaa cgcgtggcgc tgttgctggc gctggcagaa gaacgcgata ttatcctgct
     5821 ggatgaatgg gcggcggatc aggatccaca cttccgtcgt gagttttatc aggtgctgct
     5881 gccgctgatg caggagatgg gtaaaactat tttcgccatc agtcatgatg atcattactt
     5941 tatccacgcc gaccgcctgc tggaaatgcg caacgggcaa cttagcgagc tgacaggcga
     6001 agagcgcgat gctgcttcgc gtgatgccgt tgcccggacg gcataatatt acgacgctga
     6061 cctggcggac tgctgccgcc aggttaatat gctaaa
//
LOCUS       DAZFLD010000235         6027 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.236, whole genome shotgun sequence.
ACCESSION   DAZFLD010000235 DAZFLD010000000
VERSION     DAZFLD010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6027
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.236"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            115..843
                     /gene="gadW"
                     /locus_tag="ACHKBL_004229"
     CDS             115..843
                     /gene="gadW"
                     /locus_tag="ACHKBL_004229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadW"
                     /protein_id="HFO5821010.1"
                     /translation="MAHVCSVILVRRSFDIHHEQQKISLHNESILLLDKNLADDFAFC
                     SLDTRRLDIEELTVCHYLQNIRQLPRNLGLHSKDRLLINQSPPIQLVTAIFDSFNDPR
                     VNSPILSKMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
                     RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYF
                     INTFRQYYGVTPHQFSQHSPGTFS"
     gene            complement(1206..4319)
                     /gene="mdtF"
                     /locus_tag="ACHKBL_004230"
     CDS             complement(1206..4319)
                     /gene="mdtF"
                     /locus_tag="ACHKBL_004230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump RND permease MdtF"
                     /protein_id="HFO5821011.1"
                     /translation="MANYFIDRPVFAWVLAIIMMLAGGLAIMNLPVAQYPQIAPPTIT
                     VSATYPGADAQTVEDSVTQVIEQNMNGLDGLMYMSSTSDAAGNASITLTFETGTSPDI
                     AQVQVQNKLQLAMPSLPEAVQQQGISVDKSSSNILMVAAFISDNGSLNQYDIADYVAS
                     NIKDPLSRTAGVGSVQLFGSEYAMRIWLDPQKLNKYNLVPSDVISQIKVQNNQISGGQ
                     LGGMPQAADQQLNASIIVQTRLQTPEEFGKILLKVQQDGSQVLLRDVARVELGAEDYS
                     TVARYNGKPAAGIAIKLATGANALDTSRAVKEELNRLSAYFPASLKTVYPYDTTPFIK
                     ISIQEVFKTLVEAIILVFLVMYLFLQNFRATIIPTIAVPVVILGTFAILSAVGFTINT
                     LTMFGMVLTIGLLVDDAIVVVENVERVIAEDKLPPKEATHKSMGQIQRALVGIAVVLS
                     AVFMPMAFMSGATGEIYRQFSITLISSMLLSVFVAMSLTPALCATILKAAPEGDHKPN
                     ALFARFNTLFEKSTQHYTDSTRSLLRCTGRYMVVYLLICAGMAVLFLRTPTSFLPEED
                     QGVFMTTAQLPSGATMVNTTKVLQQVTDYYLTKEKNNVQSVFTVGGFGFSGQGQNNGL
                     AFISLKPWSERVGEENSVTAIIQRAMIALSSINKAVVFPFNLPAVAELGTASGFDMEL
                     LDNGNLGHEKLTQARNELLSLAAQSPNQVIGVRPNGLEDTPMFKVNVNAAKAEAMGVA
                     LSDINQTISTAFGSSYVNDFLNQGRVKKVYVQAGTPFRMLPDNINQWYVRNASGTMAP
                     LSAYSSTEWTYGSPRLERYNGIPSMEILGEAAAGKSTGDAMKFMADLVAKLPAGVGYS
                     WTGLSYQEALSSNQAPALYAISLVVVFLALAALYESWSIPFSVMLVVPLGVVGALLAT
                     DLRGLSNDVYFQVGLLTTIGLSAKNAILIVEFAVEMMQKEGKTPVEAIIEAARMRLRP
                     ILMTSLAFILGVLPLVISHGAGSGAQNAVGTGVMGGMFAATVLAIYFVPVFFVVVEHL
                     FARFKKA"
     gene            complement(4344..5453)
                     /gene="mdtE"
                     /locus_tag="ACHKBL_004231"
     CDS             complement(4344..5453)
                     /gene="mdtE"
                     /locus_tag="ACHKBL_004231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015870489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtE"
                     /protein_id="HFO5821012.1"
                     /translation="MLTACDDKSAENAAAMTPEVGVVTLSPGSVNVLSELPGRTVPYE
                     VAEIRPQVGGIIIKRNFIEGDKVNQGDSLYQIDPAPLQAELNSAKGSLAKALSTASNA
                     RITFNRQASLLKTNYVSRQDYDTARTQLNEAEANVTVAKAAVEQATINLQYANVTSPI
                     TGVSGKSSVTVGALVTANQADSLVTVQRLDPIYVDLTQSVQDFLRMKEEVASGQIKQV
                     QGSTPVQLNLENGKRYSQTGTLKFSDPTVDETTGSVTLRAIFPNPNGDLLPGMYVTAL
                     VDEGSRQNVLLVPQEGVTHNAQGKATALILDKDDVVQLREIEASKAIGDQWVVTSGLQ
                     AGDRVIVSGLQRIRPGIKARAISSSQENASTESKQ"
     gene            5561..5839
                     /locus_tag="ACHKBL_004232"
     CDS             5561..5839
                     /locus_tag="ACHKBL_004232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5821013.1"
                     /translation="MRDLQTSGIVGLSASKVGYRHSDRHGEVNDKKNVAVLPTVPASI
                     RATKGSTGGYIYQGNKVVFASPLVITGGNEISICIPRHASHQFQLLMS"
     gene            complement(5840..>6027)
                     /gene="gadE"
                     /locus_tag="ACHKBL_004233"
     CDS             complement(5840..>6027)
                     /gene="gadE"
                     /locus_tag="ACHKBL_004233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HFO5821014.1"
                     /translation="CFSHNQFKIMQLILKNKNESNITSTLNISQQTLKIQKFNIMYKL
                     KLRRMSDIVTLGITSYF"
BASE COUNT         1486 a         1500 c         1511 g         1530 t
ORIGIN      
        1 ataaaatttg gtcctaccaa atatggcagt ttttgcacac agaaacagtc tggcgtcatt
       61 tcattagtat actgacattg aaataatcgc agtaatgaaa tataagggat agtcatggct
      121 catgtctgct cggtgatcct cgttcgtcgt tcattcgata ttcatcatga acagcaaaaa
      181 atatcgttgc ataacgagag tatcctgctg ctggataaaa atttggcaga cgattttgcg
      241 ttttgttcac tggatacgcg acggctggat atcgaagagc tgacagtttg ccattactta
      301 caaaatattc gtcagttgcc acgcaattta ggattgcata gcaaagaccg tttgttaatt
      361 aaccagtcac cccccataca gctggtgacg gcgatttttg atagtttcaa tgacccccgg
      421 gtcaattcgc cgatactgag caaaatgctc tatctttcct gtttatcaat gttttctcat
      481 aagaaagaac tgatcccctt acttttcaat agtatcagta ctgtttcagg aaaagttgaa
      541 cgccttatta gctttgatat cgctaaacgt tggtatctac gcgatatcgc agaaagaatg
      601 tacaccagcg agagtctcat caaaaaaaag ttgcaggatg aaaatacctg tttcagtaaa
      661 atattactcg cctccaggat gtcgatggcc agacgattac tcgagttacg tcaaatacct
      721 ctgcatacta ttgcggaaaa atgtggctat agcagtacgt catactttat aaatacattt
      781 agacaatatt atggtgtaac gccacatcag ttttcgcaac attcgccggg taccttttcc
      841 tgacatattt cgcatttgaa tattggtcag gatctcacgt ctgcttcatg tgaaactctt
      901 tcctgatgat ttctgccggg ctaccggcta gttctctttc gcagtttgcc aggcggtata
      961 ggtattttca ggaggaaaag actcggcatg tttgggatta ttaagctaac aattcatacg
     1021 attacgggaa tgtgggtcag tattgtgctg ttcaaattga tgacgaatgg ctggagtggt
     1081 ttctactttc agtgctgtgt actaagcctg gtattcctga cggttagctg gttgttgagt
     1141 ggtgaatggt tagcaggaaa gagtaaggct gaaccctcac gttcaacctt actctcattt
     1201 acacgttacg cttttttaaa gcgggcaaag agatgttcca ctacaacgaa aaagaccgga
     1261 acgaagtaaa ttgccagcac tgttgcggca aacatcccgc ccattacgcc ggtacctacc
     1321 gcgttttgcg cgccagagcc ggcaccatga ctgataacca gcggcagcac gccgagaata
     1381 aaggccagag aagtcatcag gattgggcgt aaacgcatac gcgctgcttc gatgattgcc
     1441 tctaccggcg ttttcccttc tttctgcatc atctcaacgg caaattcgac aatcaggatg
     1501 gcgttcttgg cggaaagccc gatggtggtc agcaaaccaa cctggaagta aacgtcatta
     1561 cttaagccgc gcagatcggt ggccagtaat gcgccaacga cgcctaacgg aacaaccaac
     1621 atcaccgaga acggaattga ccagctttca taaagtgcgg cgagggcgag gaacaccacg
     1681 accagtgaaa tggcatatag cgcgggagcc tggtttgagg ataacgcttc ctgatacgac
     1741 agcccggtcc atgagtagcc gacgcctgcc ggaagttttg cgaccaggtc tgccataaat
     1801 ttcatggcgt caccggtact tttcccggca gccgcttcac ctaaaatctc cattgacggg
     1861 atgccgttgt agcgttccag tcgcggtgaa ccataggtcc attcggtaga cgaataggca
     1921 gaaagcggtg ccatcgtgcc agaggcgttg cgtacatacc attggttgat gttatccggc
     1981 aacatacgga acggcgtgcc tgcctggaca tacacttttt tcacccgccc ctggttgagg
     2041 aagtcgttca cgtagctgct gccgaaggcg gtggaaattg tctggttgat atcagacagc
     2101 gccacaccca tagcctcggc tttcgcagcg ttgacgttca ctttgaacat cggcgtatct
     2161 tccaggccgt tcgggcgtac cccgatgacc tgattcggtg attgcgctgc cagtgataac
     2221 agctcgtttc gcgcctgggt cagcttttcg tgccccagat tgccgttgtc cagcagttcc
     2281 atatcaaaac ctgacgcggt acccagttca gccaccgcgg gtaagttgaa cgggaagacg
     2341 acggctttat tgatactgct tagcgcaatc attgcccgct gaataatcgc ggtaaccgag
     2401 ttttcctcgc cgacacgttc agaccacggc ttgaggctga taaacgccag accgttgttt
     2461 tgcccctgac cactgaagcc aaagccacca acggtaaaca ccgactgaac attatttttc
     2521 tcttttgtca gataataatc cgtcacctgt tgtagcactt tcgtggtgtt aaccatggtt
     2581 gcaccggaag gtaactgcgc ggtggtcata aataccccct gatcctcttc tggtaagaaa
     2641 gaggtcggcg tacgcaggaa cagcaccgcc atcccggcgc aaatcagcag gtagaccacc
     2701 atgtagcgac cggtacaacg caacagcgag cgggtgctgt cggtatagtg ctgagttgat
     2761 ttttcaaaca gcgtgttgaa gcgtgcgaac agggcgttag gtttgtgatc gccctccggc
     2821 gcggctttca gaatggtggc gcacagggca ggggtcaggc tcattgctac aaatactgaa
     2881 agcagcatgg aggagatcag cgtgatggag aactggcggt agatctcccc ggtcgcaccg
     2941 ctcataaagg ccatcggcat aaacactgcg gaaagaacaa cggcaatccc gaccagcgca
     3001 cgttggatct gccccatcga tttatgcgtc gcttccttcg gcggtagctt atcttccgca
     3061 atgacacgct cgacgttctc caccaccacg atggcgtcat ccaccagtaa ccctatcgtc
     3121 agcaccatcc cgaacatagt taacgtgttg atggtgaaac cgaccgccga caagatcgca
     3181 aacgtcccga gaataaccac cggtacggca atcgtcggga tgattgtggc gcggaaattc
     3241 tgcaaaaaca gatacatgac caggaagacg aggatgatag cctcaaccag cgttttgaaa
     3301 acttcctgaa tagaaatttt gataaacggt gtggtgtcgt aaggataaac cgtcttcagg
     3361 cttgcaggga aataagccga taaacggttc agctcctctt tcaccgctcg cgaggtatcc
     3421 agggcgtttg ctccggtagc cagtttgatg gcgatcccgg cagcaggttt gccgttatag
     3481 cgcgccacgg tggaataatc ttccgcccca agttcgacgc gggcgacatc acgcagcagt
     3541 acttgcgaac catcttgctg aactttcaac aggattttgc caaattcttc cggcgtttgc
     3601 agacgcgtct gcacaatgat cgaggcgttc agttgctggt ctgccgcctg tggcatgcca
     3661 cccagttgac caccggaaat ctggttattt tgcaccttaa tctgggaaat aacatcggaa
     3721 ggtaccaggt tatatttatt gagtttttgc gggtccagcc agatacgcat ggcgtactcg
     3781 gaaccaaaga gctgtacgct accaacgccc gcggtacggc ttagcgggtc tttgatatta
     3841 gacgctacat agtccgcgat atcgtactgg ttgaggctgc cgttatcaga aataaatgcc
     3901 gctaccatca ggatattact gctcgactta tcgacgctaa tcccctgctg ctgcaccgct
     3961 tcaggtaatg aaggcatagc gagctgcagt ttattttgca cctgaacctg tgcgatatca
     4021 ggagatgtcc cagtctcgaa ggtcagagtg atagaggcat tacccgccgc atcactggtt
     4081 gaagacatgt acatcaggcc atcaagccca ttcatatttt gctcaatcac ctgagtgacc
     4141 gagtcttcta ccgtttgcgc atcggcaccc ggataggttg cgctgacggt aatggtcggt
     4201 ggcgcaatct gcggatactg cgcaaccggt aagttcatga tcgccagacc acctgcaagc
     4261 atcataataa tggcaagtac ccaggcaaaa accgggcgat caataaaata gttagccatg
     4321 aaagtcccct taagcctgca acgttattgt ttcgattcgg tgctggcgtt ttcctggctg
     4381 gaggaaattg ctcgtgcttt gatacccgga cgaatgcgtt gcaaaccgga aacgatcacc
     4441 cgatcgccag cctgcaagcc agaggtgaca acccactggt cgccgatggc tttgctggct
     4501 tcaatttcgc gtagctgcac aacatcgtct ttatccagaa tgagcgccgt tgctttaccc
     4561 tgggcgttgt gggtgacgcc ttcctgcggc accagtaata cattctggcg gctaccttca
     4621 tccactaatg ccgtgacgta catgccaggc agcaagtcac catttgggtt ggggaaaatc
     4681 gcccgtaacg tcacggagcc cgtggtttca tccaccgtcg ggtcggagaa tttcagcgtg
     4741 ccggtctggc tgtaacgttt accattttcc agattgagct gtactggcgt actgccctga
     4801 acctgtttga tttgcccact ggcgacctct tctttcatgc gcaagaaatc ctgcaccgac
     4861 tgcgtgagat cgacataaat cgggtccaga cgttgtacgg taaccagcga atctgcctga
     4921 ttagcggtaa cgagtgcgcc gacggtcacc gacgatttcc cgctgacgcc cgtaatcggc
     4981 gaggtgacat ttgcgtattg caggttgata gtcgcctgtt caacagccgc tttggcgacg
     5041 gtgacattgg cttctgcttc attcaactgg gtgcgcgcgg tgtcgtaatc ctgacggcta
     5101 acgtagttgg tcttcagcaa cgatgcctgg cggttaaagg tgatgcgggc attgctggcg
     5161 gtagagagcg ctttcgccag cgagcctttg gcggagttca gctctgcctg taaaggcgca
     5221 ggatcaatct gatacagcga atcgccctgg ttcactttat cgccttcgat aaagttgcgt
     5281 ttaatgataa taccgcccac ctggggacgt atctcggcaa cttcataagg aacggttcta
     5341 ccaggcaatt cgctcaacac attgaccgaa ccgggggaga gtgtgacgac accgacctca
     5401 ggcgtcatgg cggcggcgtt ttccgccgat ttgtcatcgc aggcggtgag catcgcgccg
     5461 cagaataaca acggtattaa cagctttctt cttctgttca ttttagtccc tgaaaattct
     5521 tgagaaataa cggttcttta aattgcgcgc gaggagagat atgagggatt tacagaccag
     5581 cggcatagtg gggttgtcag ccagtaaggt gggatacagg cacagtgatc gacatggtga
     5641 ggtcaacgac aaaaaaaatg ttgccgttct gccaacagtt cctgccagca ttcgggcaac
     5701 gaagggttct actggtggat acatatacca ggggaataaa gtcgttttcg cctctccttt
     5761 agtaatcacc gggggtaatg aaatcagcat ctgcatccct cgtcatgcca gccatcaatt
     5821 tcagttgctt atgtcctgac taaaaataag atgtgatacc cagggtgacg atgtcgctca
     5881 tacgtcttag tttcagtttg tacataatgt tgaatttctg aatctttaat gtttgttgcg
     5941 aaatattgag cgtcgacgtg atattgcttt catttttatt tttcagaatc agttgcataa
     6001 ttttgaactg attatgtgaa aaacata
//
LOCUS       DAZFLD010000236         6018 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.237, whole genome shotgun sequence.
ACCESSION   DAZFLD010000236 DAZFLD010000000
VERSION     DAZFLD010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6018)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6018)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6018
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.237"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            120..977
                     /gene="focA"
                     /locus_tag="ACHKBL_004234"
     CDS             120..977
                     /gene="focA"
                     /locus_tag="ACHKBL_004234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HFO5821015.1"
                     /translation="MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVF
                     ISIAFVFYITATTGTGTMPFGMAKLVGGICFSLGLILCVVCGADLFTSTVLIVVAKAS
                     GRITWGQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
                     FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIP
                     MGIVIRDFASPEFWTAVGSAPENFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWV
                     IYLRENDHH"
     gene            1032..3314
                     /gene="pflB"
                     /locus_tag="ACHKBL_004235"
     CDS             1032..3314
                     /gene="pflB"
                     /locus_tag="ACHKBL_004235"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFO5821016.1"
                     /translation="MSELNEKLATAWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFL
                     AGATEATTTLWDKVMEGVKLENRTHAPVDFDTAVASTITSHDAGYINKALEKVVGLQT
                     EAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKYAQFTSLQADLENGVNLEQTI
                     RLREEIAEQHRALGQMKEMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMS
                     FGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSGDPIW
                     ATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVS
                     IDTSSLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGG
                     VDEKLKMQVGPKSEPIKGDLLNYDEVMERMDHFMDWLAKQYITALNIIHYMHDKYSYE
                     ASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQ
                     FGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGR
                     RAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDDEVR
                     KTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRF
                     NSLTKEQQQDVITRTFTQSM"
     gene            3506..4246
                     /gene="pflA"
                     /locus_tag="ACHKBL_004236"
     CDS             3506..4246
                     /gene="pflA"
                     /locus_tag="ACHKBL_004236"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HFO5821017.1"
                     /translation="MSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWD
                     THGGKEVTVEDLMREVVTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHT
                     CLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAKYLANK
                     NVKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYK
                     LDGVKPPKKETMERVKGILEQYGHKVMF"
     gene            4345..4455
                     /locus_tag="ACHKBL_004237"
                     /pseudo
     CDS             4345..4455
                     /locus_tag="ACHKBL_004237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415420.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(4456..5886)
                     /gene="ycaM"
                     /locus_tag="ACHKBL_004238"
     CDS             complement(4456..5886)
                     /gene="ycaM"
                     /locus_tag="ACHKBL_004238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFO5821018.1"
                     /translation="MAGNVQEKQLRWYNIALMSFITVWGFGNVVNNYANQGLVVVFSW
                     VFIFALYFTPYALIVGQLGSTFKDGKGGVSTWIKHTMGPGLAYLAAWTYWVVHIPYLA
                     QKPQAILIALGWAMKGDGSLIKEYSVVALQGLTLVLFIFFMWVASRGMKSLKIVGSVA
                     GIAMFVMSLLYVAMAVTAPAITEVHIATTNITWETFIPHIDFTYITTISMLVFAVGGA
                     EKISPYVNQTRNPGKEFPKGMLCLAVMVAVCAILGSLAMGMMFDSRNIPDDLMTNGQY
                     YAFQKLGEYYNMGNTLMVIYAIANTLGQVAALVFSIDAPLKVLLGDADSKYIPASLCR
                     TNASGTPVNGYFLTLVLVAILIMLPTLGIGDMNNLYKWLLNLNSVVMPLRYLWVFVAF
                     IAVVRLAQKYKPEYVFIRNKPLAMTVGIWCFAFTAFACLTGIFPKMEAFTAEWTFQLA
                     LNVATPFVLVGLGLIFPLLARKANSK"
BASE COUNT         1614 a         1514 c         1420 g         1470 t
ORIGIN      
        1 tgcataaaaa ccatgcgagt tacgggccta taagccaggc gagatatgat ctatatcaat
       61 ttctcatcta taatgctttg ttagtatctc gtcgccgact taataaagag agagttagtg
      121 tgaaagctga caaccctttt gatcttttac ttcctgcagc aatggccaaa gtggccgaag
      181 aggcaggtgt ctataaagca acgaaacatc cgcttaagac tttctatctg gcgattaccg
      241 ccggtgtttt catctcaatc gcattcgtct tctatatcac agcaaccact ggcacaggaa
      301 caatgccctt cggcatggca aaactggttg gcggcatttg cttctctctg gggctgattc
      361 tttgtgttgt ctgcggagcc gatctcttta cttccacagt gttgattgtt gttgctaagg
      421 cgagtgggcg catcacctgg ggtcagttag cgaaaaactg gctaaatgtc tattttggca
      481 acctggtcgg cgcactgctg tttgtacttt taatgtggct ttccggcgag tatatgaccg
      541 cgaatggtca atggggacta aacgtcctac aaaccgccga ccacaaagtg caccatactt
      601 ttattgaggc cgtctgtctt ggtatcctgg caaacctgat ggtatgtctg gcagtatgga
      661 tgagttattc tggccgcagc ctgatggata aagcgttcat tatggtgctg ccggtcgcga
      721 tgtttgttgc cagcggtttt gagcacagta tcgcaaacat gtttatgatc ccgatgggta
      781 ttgtaatccg cgacttcgca tctccggaat tctggaccgc tgtcggttct gcaccggaaa
      841 atttttctca cctgaccgtg atgaacttca tcactgataa cctgattccg gttacgatcg
      901 gtaatattat cggcggtggt ttgttggttg ggttgacata ctgggtcatt tatctgcgtg
      961 aaaacgatca ccattaatgg ttgtcgaagt acgcagtaaa taaaaaatcc acttaagaag
     1021 gtaggtgtta catgtccgag cttaatgaaa agttagccac agcctgggaa ggttttacca
     1081 aaggtgactg gcagaatgaa gtaaacgtcc gtgacttcat tcagaaaaac tacactccgt
     1141 acgagggtga cgagtccttc ctggctggcg ctactgaagc gaccaccacc ctgtgggaca
     1201 aagtaatgga aggcgttaaa ctggaaaacc gcactcacgc gccagttgac tttgacaccg
     1261 ctgttgcttc caccatcacc tctcacgacg ctggctacat caacaaagcg ttggaaaaag
     1321 ttgttggtct gcagactgaa gctccgctga aacgtgctct tatcccgttc ggtggtatca
     1381 aaatgatcga aggttcctgc aaagcgtaca accgcgaact ggacccgatg atcaaaaaaa
     1441 tcttcactga ataccgtaaa actcacaacc agggcgtgtt cgacgtttac actccggaca
     1501 tcctgcgttg ccgtaaatcc ggtgttctga ccggtctgcc agatgcttat ggccgtggtc
     1561 gtatcatcgg tgactaccgt cgcgttgcgc tgtacggtat cgactatctg atgaaagaca
     1621 aatacgctca gttcacctct ctgcaggctg atctggaaaa cggcgtaaac ctggaacaga
     1681 ctatccgtct gcgcgaagaa atcgctgaac agcaccgcgc tctgggtcag atgaaagaaa
     1741 tggctgcgaa atacggctac gacatctctg gtccggctac caacgctcag gaagctatcc
     1801 agtggactta cttcggctac ctggctgctg ttaagtctca gaacggtgct gcaatgtcct
     1861 tcggtcgtac ctccaccttc ctggatgtgt acatcgaacg tgacctgaaa gctggcaaga
     1921 tcaccgaaca agaagcgcag gaaatggttg accacctggt catgaaactg cgtatggttc
     1981 gcttcctgcg tactccggaa tacgatgaac tgttctctgg cgacccgatc tgggcaaccg
     2041 aatctatcgg tggtatgggc ctcgacggtc gtaccctggt taccaaaaac agcttccgtt
     2101 tcctgaacac cctgtacacc atgggtccgt ctccggaacc gaacatgacc attctgtggt
     2161 ccgaaaaact gccgctgaac ttcaagaaat tcgctgctaa agtgtccatc gacacctctt
     2221 ctctgcagta tgagaacgat gacctgatgc gtccggactt caacaacgat gactacgcta
     2281 tcgcttgctg cgtaagcccg atgatcgttg gtaaacaaat gcagttcttc ggtgcgcgtg
     2341 caaacctggc gaaaaccatg ctgtacgcaa tcaacggcgg cgttgacgaa aaactgaaaa
     2401 tgcaggttgg tccgaagtct gagccgatca aaggcgatct cctgaactac gatgaagtga
     2461 tggagcgcat ggatcacttc atggactggc tggctaaaca gtacatcact gcactgaaca
     2521 tcatccacta catgcacgac aagtacagct acgaagcctc tctgatggcg ctgcacgacc
     2581 gtgacgttat ccgcaccatg gcgtgtggta tcgctggtct gtccgttgct gctgactccc
     2641 tgtctgcaat caaatatgcg aaagttaaac cgattcgtga cgaagacggt ctggctatcg
     2701 acttcgaaat cgaaggcgaa tacccgcagt ttggtaacaa cgatccgcgt gtagatgacc
     2761 tggctgttga cctggtagaa cgtttcatga agaaaattca gaaactgcac acctaccgtg
     2821 acgctatccc gactcagtct gttctgacca tcacttctaa cgttgtgtat ggtaagaaaa
     2881 ctggtaacac cccagacggt cgtcgtgctg gcgcgccgtt cggaccgggt gctaacccga
     2941 tgcacggtcg tgaccagaaa ggtgcagtag cctctctgac ttccgttgct aaactgccgt
     3001 ttgcttacgc taaagatggt atctcctaca ccttctctat cgttccgaac gcactgggta
     3061 aagacgacga agttcgtaag accaacctgg ctggtctgat ggatggttac ttccaccacg
     3121 aagcgtccat cgaaggtggt cagcacctga acgttaacgt gatgaaccgt gaaatgctgc
     3181 tcgacgcgat ggaaaacccg gaaaaatatc cgcagctgac catccgtgta tctggctacg
     3241 cagtacgttt caactcgctg actaaagaac agcagcagga cgttattact cgtaccttca
     3301 ctcaatctat gtaattagat ttgactgaaa tcgtacagta aaaagcgtac aataaaggct
     3361 ccacgaaagt ggggcctttt ttagcacgag agcctttttt gtcagctatc tatactttaa
     3421 ggtgactgcc aaaacagact cgacgtagcc ttcgagctgc gcaccaacac ggcctcagat
     3481 gggccacatc tggagaaaca ccgcaatgtc agttattggt cgcattcact cctttgaatc
     3541 ctgtggaacc gtagacggtc cgggtattcg ctttatcacc tttttccagg gctgcctgat
     3601 gcgctgcctg tattgtcata accgcgacac ctgggacacg catggcggta aagaagttac
     3661 cgttgaagat ttaatgagag aggtggtgac ctatcgccac tttatgaacg cttccggcgg
     3721 cggcgttacc gcatccggcg gtgaagcaat cctgcaagct gagtttgttc gtgactggtt
     3781 ccgcgcctgc aaaaaagaag gcattcatac ctgtctggac accaacggtt ttgttcgtcg
     3841 ttacgatccg gtgattgatg aactgctgga agtaaccgac ctggtaatgc tcgatctcaa
     3901 acagatgaac gacgagatcc accaaaatct ggttggagtt tccaaccacc gcacgctgga
     3961 gttcgccaaa tatctggcga acaaaaatgt gaaggtgtgg atccgctacg ttgttgtccc
     4021 aggctggtct gacgatgacg attcagcgca tcgcctcggt gaatttaccc gtgatatggg
     4081 caacgttgag aaaatcgagc ttctccccta ccacgaactg ggcaaacaca aatgggtggc
     4141 gatgggtgaa gaatacaaac ttgatggtgt taaaccaccg aagaaagaga ccatggaacg
     4201 cgtgaaaggc attcttgagc agtacggtca taaggtaatg ttctaatttc cccgtaaagc
     4261 ggcaactttt ttgagttgcc gcttttttat tccgcgctgc ataaataatt cagagctcta
     4321 tttctaacgc ccttacagca cagcttccaa atgtattacc caaaccgcca gtatatgtcc
     4381 tgcggcttgc gcgcatttct cgattatgta aaaacgggtc agatctgcca gagtcagcat
     4441 caccgaccac aataattatt tgctattcgc tttacgcgcc agcagcggga atatcagccc
     4501 taatcctacc agcacaaacg gcgttgcaac attcagcgcc aactggaatg tccactctgc
     4561 ggtgaaggct tccattttcg ggaaaatacc agtcaggcag gcaaaagcgg taaaggcaaa
     4621 acaccaaatt ccgacggtca ttgccagcgg cttattacga ataaagacat attccggctt
     4681 atatttctgc gccaggcgaa caactgcaat aaatgcaaca aatacccata aataacgcag
     4741 cggcatcact accgagttaa gattcaacag ccatttatag agattgttca tatcaccaat
     4801 gccgagagtc ggtagcatta tcaggatcgc caccagcacc agggtcagaa aatagccatt
     4861 aacgggcgta ccagaagcgt tggtacgaca taaactggct ggaatatatt tgctgtcagc
     4921 atcgcctaat agcactttaa gcggggcatc aatcgagaat accagcgcag ctacttgtcc
     4981 cagggtattc gcaatggcgt aaatcaccat taaagtatta cccatgttgt aatactcgcc
     5041 cagcttctga aaggcgtaat actgaccgtt agtcattaag tcatccggga tattacgcga
     5101 gtcaaacatc atccccatcg ccagtgaacc cagaatggca caaaccgcaa ccatcaccgc
     5161 caggcataac atcccttttg gaaattcttt tcctgggttg cgcgtttgat taacgtaagg
     5221 agaaatcttc tctgctccgc caaccgcgaa aaccagcatt gaaatagtgg taatgtaggt
     5281 aaagtcgata tgaggaatga acgtttccca ggtaatgttt gtggtcgcaa tatgcacttc
     5341 agtaattgca ggcgcggtta ccgccatcgc cacatacagg agcgacataa caaacatcgc
     5401 aatccctgcc acagaaccga cgattttcag cgatttcata ccgcgtgaag caacccacat
     5461 aaagaagata aacagcacca gcgttaaccc ctgtaacgct acgactgaat attctttgat
     5521 taacgaaccg tcgcctttca tcgcccaacc gagcgcaatc agtattgcct ggggtttttg
     5581 tgccagatag ggaatatgca ctacccagta ggtccacgcg gcgaggtaag ccagtccggg
     5641 tcccatcgtg tgtttaatcc aggtactgac cccgcccttc ccatctttga aggtcgagcc
     5701 taactgacca acaattagcg cataaggtgt gaaatagagc gcaaagataa acacccatga
     5761 aaaaacaacc accagcccct ggttggcgta gttattaaca acattgccaa aaccccagac
     5821 agtgataaaa gacatcagcg caatgttgta ccatcgcagc tgtttttcct gaacattccc
     5881 agccatagac aaatccttcg ccaaaattaa aggggcatct gtaaaacgag ggattatgcg
     5941 cggcaaataa gaagtgcgga aatagcgaaa tgcaaaatta taacctgctg cacattttat
     6001 ttttaaacga ataaaatt
//
LOCUS       DAZFLD010000237         5982 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.238, whole genome shotgun sequence.
ACCESSION   DAZFLD010000237 DAZFLD010000000
VERSION     DAZFLD010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5982)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5982)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5982
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.238"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            113..1531
                     /gene="araE"
                     /locus_tag="ACHKBL_004239"
     CDS             113..1531
                     /gene="araE"
                     /locus_tag="ACHKBL_004239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-proton symporter AraE"
                     /protein_id="HFO5821019.1"
                     /translation="MVTINTESALTPRSLRDTRRMNMFVSVAAAVAGLLFGLDIGVIA
                     GALPFITDHFVLTSRLQEWVVSSMMLGAAIGALFNGWLSFRLGRKYSLMAGAILFVLG
                     SIGSAFATSVEMLIAARVVLGIAVGIASYTAPLYLSEMASENVRGKMISMYQLMVTLG
                     IVLAFLSDTAFSYSGNWRAMLGVLALPAVLLIILVVFLPNSPRWLAEKGRHIEAEEVL
                     RMLRDTSEKAREELNEIRESLKLKQGGWALFKINRNVRRAVFLGMLLQAMQQFTGMNI
                     IMYYAPRIFKMAGFTTTEQQMIATLVVGLTFMFATFIAVFTVDKAGRKPALKIGFSVM
                     ALGTLVLGYCLMQFDNGTASSGLSWLSVGMTMMCIAGYAMSAAPVVWILCSEIQPLKC
                     RDFGITCSTTTNWVSNMIIGATFLTLLDSIGAAGTFWLYTALNIAFVGITFWLIPETK
                     NVTLEHIERKLMAGEKLRNIGV"
     gene            1660..2352
                     /gene="ygeA"
                     /locus_tag="ACHKBL_004240"
     CDS             1660..2352
                     /gene="ygeA"
                     /locus_tag="ACHKBL_004240"
                     /EC_number="5.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HFO5821020.1"
                     /translation="MKTIGLLGGMSWESTIPYYRLINEGIKQRLGGLHSAQVLLHSVD
                     FHEIEECQRRGEWDKTGDILAEAALGLQRAGAEGIVLCTNTMHKVADAIESRCSLPFL
                     HIADATGRAIIRAGMTRVALLGTRYTMEQDFYRGRLTEQFSINCLIPEADKRAKINQI
                     IFEELCLGQFTEASRTYYAQVIARLAEQGAQGVIFGCTEIGLLVPEERSVLPVFDTAA
                     IHAEDAVAFMLS"
     gene            complement(2339..3274)
                     /gene="lysR"
                     /locus_tag="ACHKBL_004241"
     CDS             complement(2339..3274)
                     /gene="lysR"
                     /locus_tag="ACHKBL_004241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator LysR"
                     /protein_id="HFO5821021.1"
                     /translation="MAAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEK
                     VIGLKLFERVRGRLHPTVQGLRLFEEVQRSWYGLDRIVSAAESLREFRQGELSIACLP
                     VFSQSFLPQLLQPFLARYPDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHTPAGTER
                     TELLSLDEVCVLPPGHPLAVKKVLTPDDFHGENYISLSRTDSYRQLLDQLFTENQVKR
                     RMIVETHSAASVCAMVRAGVGVSVVNPLTALDYAASGLVVRRFSIAVPFTVSLIRPLH
                     RPSSALVQAFSEHLQAGLPKLVTSLDAILSSATTA"
     gene            3396..4658
                     /gene="lysA"
                     /locus_tag="ACHKBL_004242"
     CDS             3396..4658
                     /gene="lysA"
                     /locus_tag="ACHKBL_004242"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HFO5821022.1"
                     /translation="MPHSLFSTDTDLTAENLLRLPAEFGCPVWVYDAQIIRRQIAALK
                     QFDVVRFAQKACSNIHILRLMREQGVKVDSVSLGEIERALAAGYNPQTHPDDIVFTAD
                     VIDQATLERVSELQIPVNAGSVDMLDQLGQVSPGHRVWLRVNPGFGHGHSQKTNTGGE
                     NSKHGIWYTDLPAALDVIQRHHLQLVGIHMHIGSGVDYAHLEQVCGAMVRQVLEFGQD
                     LQAISAGGGLSVPYQQGEEAVDTEHYYGLWNAARQQIARHLGHPVKLEIEPGRFLVAQ
                     SGVLITQVRSVKQMGSRHFVLVDAGFNDLMRPAMYGSYHHISALAADGRSLEHAPTVE
                     TVVAGPLCESGDVFTQQEGGNVETRALPEVKAGDYLVLHDTGAYGASMSSNYNSRPLL
                     PEVLFDNGQARLIRRRQTIEELLALELL"
     gene            complement(4665..5696)
                     /gene="galR"
                     /locus_tag="ACHKBL_004243"
     CDS             complement(4665..5696)
                     /gene="galR"
                     /locus_tag="ACHKBL_004243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalR"
                     /protein_id="HFO5821023.1"
                     /translation="MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLS
                     YHPNANARALAQQTTETVGLVVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQ
                     KERQAIEQLIRHRCAALVVHAKMIPDADLASLMKQMPGMVLINRILPGFENRCIALDD
                     RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIPANDRLVTFGE
                     PDESGGEQAMTELLGRGRNFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVL
                     VSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFSPTLVRRHSVSTPSL
                     EASHHATSD"
BASE COUNT         1384 a         1428 c         1647 g         1523 t
ORIGIN      
        1 taacgacatg tcgcagcaat ttaatccata tttatgctat ttccgacctg acacctgtgt
       61 gaattgttca cgtatttttt cactatgtct tactctctgc tggcaggaaa aaatggttac
      121 tatcaatacg gaatctgctt taacgccacg ttctttgcgg gatacgcggc gtatgaatat
      181 gtttgtttcg gtagctgctg cggtcgcagg attgttattt ggtcttgata tcggcgtaat
      241 cgccggagcg ttgccgttca ttaccgatca ctttgtgctg accagccgtt tgcaggaatg
      301 ggtggtcagt agcatgatgc tcggcgcagc aattggtgcg ctgtttaatg ggtggctgtc
      361 gttccgcctg gggcgtaaat acagcctgat ggcgggggct atcctttttg tgctcggttc
      421 gatagggtcc gcatttgcga ccagcgtaga gatgttaatc gccgctcgtg tggtgctggg
      481 cattgctgtc gggatcgcgt cttacaccgc tcctctgtat ctttctgaaa tggcaagtga
      541 aaacgttcgc ggtaagatga tcagtatgta ccagttgatg gtcacactcg gcatcgtgct
      601 ggcgttttta tccgatacag cgttcagtta tagcggtaac tggcgcgcaa tgttgggggt
      661 tcttgcttta ccagcagtcc tgctgattat tctggtggtc ttcctgccaa atagcccgcg
      721 ctggctggcg gaaaaagggc gtcatattga ggcggaagaa gtgttgcgta tgctgcgcga
      781 tacgtcggaa aaagcgcgag aagaactcaa cgaaattcgt gaaagcctga agttaaaaca
      841 gggcggttgg gcactgttta agatcaaccg taacgtccgt cgtgctgtgt ttctcggtat
      901 gttgttgcag gcgatgcagc agtttaccgg tatgaacatc atcatgtact acgcgccacg
      961 tatcttcaaa atggcgggct ttacgaccac agaacaacag atgattgcga ctctggtcgt
     1021 ggggctgacc tttatgttcg ctaccttcat tgcggtcttt acggtagata aagcagggcg
     1081 taagccggct ctgaaaattg gtttcagcgt gatggcgtta ggcactctgg tgctgggtta
     1141 ctgcctgatg cagtttgata acggtacggc ttccagtggc ttgtcctggc tctctgttgg
     1201 catgacgatg atgtgtattg ccggttatgc gatgagcgcc gcgccagtgg tgtggatctt
     1261 gtgctctgaa attcagccgc tgaaatgccg cgattttggc atcacctgct caacgacgac
     1321 aaactgggtg tcgaatatga ttatcggcgc gaccttcctg acactgcttg atagcattgg
     1381 cgctgccggt acgttctggc tctacactgc gctgaacatt gcgtttgtgg gcatcacttt
     1441 ctggctcatt ccggaaacca aaaatgtcac gctggaacat atcgagcgca aactgatggc
     1501 aggcgaaaag ttgagaaata tcggcgtctg atttcacggg ccggatgtgc tgtacatccg
     1561 gccctttttt cgttaataga gattaggcac ctggccgttg aggcgtttgt ctcgttcctt
     1621 attcagcctt gttgcggtaa cacacatcag gagagaggaa tgaaaacaat tggtttgctg
     1681 ggaggaatga gctgggaatc cactattcct tactatcgtc tgataaatga aggcattaaa
     1741 cagcggcttg gtgggcttca ctctgcgcaa gtgctgctac atagcgtcga ttttcatgaa
     1801 atagaagagt gccagcgtcg cggtgagtgg gacaaaaccg gggatattct ggctgaggcg
     1861 gcgcttggct tacagcgggc gggcgcagaa ggtattgtgt tatgcaccaa tacgatgcat
     1921 aaagtggcgg atgccattga gtctcgttgc tctctgcctt tcttacacat agcggatgcc
     1981 actggacgtg caattatccg ggctggaatg actcgtgtgg cgctgctggg tacgcgttac
     2041 accatggaac aggattttta tcgcgggcgg ctgacggaac aattttccat caactgtctc
     2101 attcctgaag cggataaacg ggcgaaaatt aatcagatta tttttgaaga actgtgtctg
     2161 gggcaattta ccgaagcgtc acgcacttat tatgcgcaag tgattgctcg ccttgcagaa
     2221 cagggcgcac agggtgtcat ttttggctgt acggaaattg gtttactggt gccagaagag
     2281 cgcagtgttc tgcctgtgtt tgatactgcg gcgatccatg ccgaggatgc tgtcgctttt
     2341 atgctgtcgt agctgacgac aaaatagcgt caagagaagt gaccagtttc ggtaatcccg
     2401 cttgtaaatg ctcactaaac gcctgaacca gcgctgatga cgggcggtgc agggggcgga
     2461 tcaggctaac ggtgaacgga accgcaatac tgaaccgccg taccactaaa ccgcttgccg
     2521 cataatccag tgcggtgagc gggttaacca ccgaaacacc tacccccgcc cgtaccattg
     2581 cgcagactga cgcggcgctg tgggtttcta cgatcatgcg tcgtttaacc tgattctcag
     2641 taaacaattg atccagcaac tggcgatagc tgtcagtacg ggaaaggctg atgtagtttt
     2701 caccgtgaaa atcatccggc gttaatacct tttttaccgc cagcggatgg cccggaggta
     2761 acacacacac ttcatctaaa gagagtaatt cggtacgttc tgttcccgca ggcgtatgga
     2821 gtgtctccgt gagtcctaaa tcatgacgct gggccgagag ccactcttca agtagcggtg
     2881 attcctgggg cacgatattt aagctgacat cgggataacg tgccagaaag ggttgcagga
     2941 gctgcggtaa aaaagattgc gaaaagaccg gcaggcaggc aatagacagt tctccctggc
     3001 gaaactcgcg cagactttct gcggcgctga caatgcgatc cagtccgtac caggatcgtt
     3061 gcacttcttc aaacagacgc agtccttgca cggtaggatg caatcgtcca cgtacgcgct
     3121 caaacaattt cagcccgatc accttctcaa agcgcgccag ttcgcggctg acagttggct
     3181 gtgaggtgtg tagcaggtgt gccgcctcag tcaggcttcc ggcggtcatt accgcatgaa
     3241 aaatttcaat atgacgtaag ttaacggcgg ccattagcgc tctctcgcaa tccggtaatc
     3301 catatcattt ttgcatagac tcgacataaa tcgatatttt ttattctttt tatgatgtgg
     3361 cgtaatcata aaaaagcact tatctggagt ttgttatgcc acattcactg ttcagcactg
     3421 ataccgatct caccgccgaa aatctgctgc gtttgcccgc agaatttggc tgcccggtgt
     3481 gggtctacga tgcgcaaatt attcgtcggc agattgcagc gctgaaacag tttgatgtgg
     3541 tgcgcttcgc acagaaagcc tgttccaata ttcatatttt gcgcttaatg cgtgagcagg
     3601 gcgtgaaagt ggattccgtc tcgttaggcg aaatagagcg tgcactggcg gcgggttaca
     3661 atccgcaaac gcaccctgat gatattgttt ttacggcaga tgttatcgat caggcgacgc
     3721 ttgaacgcgt cagtgaattg caaattccgg tgaatgcggg ttctgttgat atgctcgacc
     3781 aactgggtca ggtttcgcca gggcatcggg tatggctgcg tgttaatccg gggtttggtc
     3841 acgggcatag ccaaaaaacc aataccggtg gcgaaaacag caagcacggt atctggtaca
     3901 ccgatctgcc cgccgcactg gacgtgatac aacgtcatca tctacagctg gtcggcattc
     3961 acatgcacat tggttctggc gtcgattatg cccatctgga acaggtatgt ggtgctatgg
     4021 tgcgtcaggt cctcgaattc ggtcaggatt tacaggctat ttctgcgggc ggtgggcttt
     4081 ctgttcctta tcaacagggt gaagaggcgg ttgataccga gcattattat ggtctgtgga
     4141 atgccgcgcg tcagcaaatc gcccgccatt tgggccaccc tgtgaaactg gaaattgaac
     4201 cgggtcgctt tctggtagcg cagtctggcg tattaattac tcaagtgcgg agcgtcaaac
     4261 aaatgggtag ccgccacttt gtgctggttg atgccgggtt caacgatctg atgcgcccgg
     4321 caatgtacgg tagttaccac catatcagtg ccctggcagc tgatggtcgt tctctggaac
     4381 acgcaccaac ggtggaaacc gtcgtcgccg ggccgttatg tgaatcgggc gatgtcttta
     4441 cccagcagga agggggaaat gtcgaaaccc gcgccttgcc ggaagtgaag gcaggtgatt
     4501 atctggtact gcatgataca ggggcatatg gcgcatcaat gtcatccaac tacaacagcc
     4561 gtccgctgtt accagaagtt ctgtttgata atggtcaggc gcggttgatt cgccgtcgcc
     4621 agaccattga agaattactg gcgctggaat tgctttaact gcggttagtc gctggttgca
     4681 tgatgacttg cctccagcga cggagttgac actgaatggc gacgtaccag cgtcggacta
     4741 aagacattag tgatttccgg gagagggcga ttatccgcca gcgccaaagc cagttcggca
     4801 gcctgggtcg ccatcgtcac gattgggtaa cgcacggtgg tcagacgcgg gcgcacatag
     4861 cgtgacacca gcacatcatc aaagccaatt aacgaaatct cacccggtac atcaatacca
     4921 ttatcattga gcacgcccat cgcgcccgcc gccattgaat cgttataaca ggctaccgca
     4981 gtgaaatttc tgcctcgtcc caaaagctcg gtcattgcct gttcgccgcc gctttcgtct
     5041 ggttcgccaa atgtcaccag ccggtcattg gccggaatac cactttcagc aagggcatcg
     5101 taataccctt gcagacgatc ttcggcgtca gaaatagagt ggttagagca cagataacca
     5161 atgcgggtat gaccttgctg aattaaatga cgcgtagcca gccaggcacc gtaacgatcg
     5221 tccagagcaa tacaacggtt ttcaaagcca ggcaggatac ggttgatcag caccataccg
     5281 ggcatttgtt tcattaatga ggctaaatcg gcatccggga tcattttggc atggacgacc
     5341 aacgcagcac agcgatggcg gatcagttgc tcaatggcct gacgctcttt ttgttcgttg
     5401 tggtaaccgt tgccaatcaa taaaaaatta ccagtgtgat aagccacctg ttcgaccgct
     5461 ttcaccattg caccgaaaaa cggatcggaa acatcaccaa cgaccagacc gaccgtttca
     5521 gtggtctgct gcgccagcgc acgggcgttg gcgttcgggt gatagctaag agactccatt
     5581 gcactatgca cagcaagccg ggaagcttcg ctggctttgg gtgaattatt aatgacgcgg
     5641 gaaacggtgg cgactgaaac gcctgccagt cgggctacat cctttatggt cgccatgaaa
     5701 ataccttagt gggtaaacgc ttacattctt tcagtgttac ggaaaacgcc cgttggttca
     5761 aggggcggcg tgttttcatt gtgggaatgt gagccaaacg caaataagta agacaaaaaa
     5821 agagattgca aacctttggt tacactttgc gaaacgctgt tgcgattgac cgctggtggc
     5881 gtttggcttc aggttgctaa agtggtgatc ccagaggtat tgataggtga agtcaacttc
     5941 gggttgagca catgaattac accagcctgc gcagatgcgc ag
//
LOCUS       DAZFLD010000238         5965 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.239, whole genome shotgun sequence.
ACCESSION   DAZFLD010000238 DAZFLD010000000
VERSION     DAZFLD010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5965)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5965)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5965
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.239"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            221..802
                     /gene="mdaB"
                     /locus_tag="ACHKBL_004244"
     CDS             221..802
                     /gene="mdaB"
                     /locus_tag="ACHKBL_004244"
                     /EC_number="1.6.5.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase MdaB"
                     /protein_id="HFO5821024.1"
                     /translation="MSNILIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHDVRIVRA
                     ESDYDVKAEVQNFLWADVVIWQMPGWWMGAPWTVKKYIDDVFTEGHGTLYASDGRTRK
                     DPSKKYGSGGLVQGKKYMLSLTWNAPMEAFTEKDQFFHGVGVDGVYLPFHKANQFLGM
                     EPLPTFIANDVIKMPDVPRYTEEYRKHLVEIFG"
     gene            833..1147
                     /gene="ygiN"
                     /locus_tag="ACHKBL_004245"
     CDS             833..1147
                     /gene="ygiN"
                     /locus_tag="ACHKBL_004245"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HFO5821025.1"
                     /translation="MITVIAEIRTRPGQHHRQAVLDQFAKIVPTVLKEEGCHGYAPMV
                     DCAAGVSFQSMAPDSIVMIEQWESIAHLEAHLQTPHMKAYSEAVKGDVLEMNIRILQP
                     GL"
     gene            complement(1192..2079)
                     /locus_tag="ACHKBL_004246"
     CDS             complement(1192..2079)
                     /locus_tag="ACHKBL_004246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFO5821026.1"
                     /translation="MIQKDKVRVDIFGERFRTRASQLTPGLRVVASYINEHREVVLEQ
                     TAMEIAATLNTSDATVIRAIQALGFAGLRDLKRTLEQWLGPALSSSEKMSTTVSNLTS
                     DVNTAIDFVLEGHLYTCNVLSEPENRHALAQAVALLVQARQVAIFGIGASGILADYTA
                     RLFNRIGLPATALNRTGIGLAEQLIALQRGDVLIMMAQKSAHREGLTMLREARRLGIP
                     VILLTNALDSRFSKDASIVIHVPRGDEKGKTPLHGTVLLCLEMIVWSVASAVPQRAVK
                     TIKRINDFHRGLKTGRKNG"
     gene            complement(2076..3023)
                     /locus_tag="ACHKBL_004247"
     CDS             complement(2076..3023)
                     /locus_tag="ACHKBL_004247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-siderophore ABC transporter
                     substrate-binding protein"
                     /protein_id="HFO5821027.1"
                     /translation="MRLFFSLLILLSFFSRATEPVQVFTDDLGRKVTVPAHPKRIVSL
                     HDLDITIPLIELGVPPVASHGRTRPDGSHFIRSGALLTGVDFDNSSIAFIGTADIDIE
                     AIVAAKPDLIITEPTRNTPIEQLEKIAPTVSIDHLKGGAPEIYRKLAGLTGTQSQLAI
                     LERRYQAQINALKATLDSQKITVSVIQANQGKINVMHSYHSLGRVLRDAGFRFPPLIE
                     SIPEGGRMDVSAERLPELDADFVFATWRGDTGGKPQDELAAMEKVMPGWCQFLTACRS
                     GRYVLISREEAISNSFASLGLMAAQIQSQIAGRPLPEAK"
     gene            complement(3034..4071)
                     /locus_tag="ACHKBL_004248"
     CDS             complement(3034..4071)
                     /locus_tag="ACHKBL_004248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311941.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFO5821028.1"
                     /translation="MNVGLRPLRVGKFSTLVRPKNLVLLGGLFLFAVGILIFGLMHGS
                     FSVPASEVGRALFAPENVSTDARYIVQDIRLPRVIMALLCGAMLGMAGAAMQSIARNG
                     LADPGLIGVKEGCSVAVLWLIFQFPMLGMFWRPVAGLAGGLLVALIVIFCARDISRPR
                     FVLIGIGVSWFFAAGIGVFMTTADVRDVQTALMWLSGSLHAANWMLVGISACWMLPAA
                     LLLLFTARTADIALLGHQVATGLGVNSSRLALLRVAAPIILTAVCVSCVGNIGFVGLI
                     APHISRFILRGGQTTLLLGSAVSGALLVILADSIGRLAFLPLQLPAGIIISLIGGPFF
                     LLLLWQRRNSF"
     gene            complement(4080..5063)
                     /locus_tag="ACHKBL_004249"
     CDS             complement(4080..5063)
                     /locus_tag="ACHKBL_004249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFO5821029.1"
                     /translation="MMRTGLVTVIFLALLLVGCVVYPGIGARFIAPQTVLQAFLHFDP
                     QNFDHNVIVRLRLPRLAAALLTGASLGVAGALLQAVIRNPLGEPHILGLNAGAALAVV
                     AASALGLAFPVGRPLLASTGGALLFLLILLLSSAGRSGLTPMKVTLCGVALSAFVSSI
                     TAAILIFDEQTLLAMRTWLAGDLAGQDWVTLGTSAWFSLGGFVLAIYLAPSLNMLALG
                     DRMAQGLGVSVLRTRTFTLLAIALLCGAAVSIAGPIGFVGLLVPQIVRRLVSADLRVL
                     LPLSACVGALLLLLADIIARTLFTPHELATGVMTALVGAPVFVIMATRMFK"
     gene            complement(5060..5869)
                     /locus_tag="ACHKBL_004250"
     CDS             complement(5060..5869)
                     /locus_tag="ACHKBL_004250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO5821030.1"
                     /translation="MAHNAKGQGLILDNVSAGYHKKIIVDGVSFSVPTEKMTVLVGAN
                     GCGKSTLLSTIARILQPMGGSILLDGKAIHEQPTKALSRRLGILPQSPLLPEGLTVYE
                     LVSRGRFPWQNFIRQWSDADEQAVEEALKLTGTQEFAHLPVEKLSGGQRQRCWIAMVL
                     AQKTPYILLDEPTTWLDLRYQVEILELLHDLTRHHGRTVVVVLHDLNFAVNYGDTLIF
                     LRQGKVVRVLNEGEHCTPELVKAVFDVDVHASINPLTGKPFFMPFRGVEKV"
BASE COUNT         1692 a         1623 c         1367 g         1283 t
ORIGIN      
        1 aaactacttt atcatttaca taatacatct ctcctgtggg aacttatagg aagttgattt
       61 cagtattgtc attttcagaa aatatttatc ccgcatcggt gaatcagagc aagatcagac
      121 ttttgctaaa ttcgcaaaag actttgcaca ttttgctaat ttcaccgtac cgctctgtga
      181 cgtactatag tcgacaaacg tctcaccttg aggttaaaaa atgagcaaca tcctgattat
      241 caacggcgcg aaaaaattcg cccactccaa tggtcaactg aacgacaccc tgaccgaagt
      301 cgcggatggc acactgcgcg acctcgggca tgatgtccgc atcgttcgcg ccgagagcga
      361 ctacgatgtc aaagccgaag tacaaaactt tctctgggct gatgtggtga tctggcagat
      421 gcctggctgg tggatgggcg cgccgtggac agtgaaaaaa tacattgatg atgtattcac
      481 cgaaggtcac gggactctgt atgccagcga tggtcgtacc cgcaaagatc cgtcgaaaaa
      541 atacggttct ggcggtctgg tacaaggtaa aaaatatatg ctttccctga cctggaacgc
      601 accaatggaa gctttcaccg aaaaagatca gttcttccac ggggttggcg ttgacggtgt
      661 gtatctgccg ttccataaag caaaccaatt ccttggtatg gaaccgctgc cgacatttat
      721 cgctaatgac gtgataaaaa tgcctgatgt tccccgctat actgaagaat atcgcaagca
      781 tcttgtggaa atttttggtt aactagagct ctggctttag aaggagttaa ccatgattac
      841 agtaatcgca gaaatccgta ctcgtcctgg tcaacatcac cgtcaggcag tgttggatca
      901 gtttgctaaa atcgttccaa ccgtactgaa agaagaaggt tgccacggct atgcgccaat
      961 ggtggattgc gctgctggcg tgagtttcca gtctatggca ccggattcta tcgtgatgat
     1021 tgagcagtgg gaaagcatcg cgcatcttga agcgcatctg caaaccccgc acatgaaagc
     1081 atatagcgaa gccgtaaaag gtgacgtgct ggagatgaat attcgtatcc tgcagccggg
     1141 gctttgacat ttctaaaaag aggccggtgc tgctaccggt ctcagcccag attagccatt
     1201 ctttcttccc gttttcagtc ctcgatggaa atcgttaatt cgcttaatgg ttttaaccgc
     1261 acgctgcggc actgcggaag ctaccgacca gacaatcatt tccagacaca acaataccgt
     1321 accatgtagc ggcgttttcc ctttttcatc cccgcgcgga acatgaatca cgatactggc
     1381 gtctttgcta aaacgtgaat ccagcgcgtt tgtcagcaaa ataacaggga tccccagccg
     1441 cctcgcctca cgaagcattg tcagcccttc ccggtgcgca gatttttgcg ccatcatgat
     1501 cagaacatcg ccacgttgaa gcgcgatcag ttgctcggca aggccaatac ccgtacggtt
     1561 tagtgccgtg gcaggcaaac ctatgcggtt gaatagtcgt gcggtatagt ccgccagaat
     1621 acccgaagcg ccaatgccaa aaatagcgac ttgccgtgcc tgcaccaaca acgctacagc
     1681 ttgcgcaagc gcgtggcggt tttcaggctc tgacaggaca ttacaggtat acaaatgccc
     1741 ttcaagaaca aagtcgattg cggtgttaac atcgctcgtc aagttactca ccgtcgtaga
     1801 catcttttcg ctggaactaa gggctggacc aagccactgt tccagagtgc gtttcagatc
     1861 acgcagcccg gcaaacccca gggcctgtat agccctgata actgtggcat cggaagtgtt
     1921 caacgtggcc gcaatttcca tcgccgtttg ctcgagtacc acttcacgat gctcattaat
     1981 gtagctggcg acaactctta acccaggcgt taactgactc gcgcgagtgc gaaaacgctc
     2041 accaaaaata tcaacccgca ctttatcttt ctggatcatt tagcctccgg tagaggtcgc
     2101 cctgcaattt gtgattgtat ctgcgcagcc atcagcccca gagatgcaaa ggagttagaa
     2161 attgcctctt cacgagaaat cagcacataa cgcccggaac gacaggcagt cagaaactga
     2221 caccatcccg gcatgacttt ttccattgct gccagctcat cctgaggttt accaccggtg
     2281 tcaccgcgcc aggttgcaaa aacaaaatcg gcatccagtt caggtaaacg ctccgcgctc
     2341 acatccattc gcccaccttc aggaatgctc tcaatcagcg gcggaaaacg aaaacccgca
     2401 tcgcgtaata cccgccccag cgaatgatag ctatgcatca cattaatttt tccctggttt
     2461 gcctgaatca ccgataccgt tattttttgg ctgtccagcg tcgccttcag cgcattgatt
     2521 tgtgcctgat agcggcgttc aagtatcgcc agctgcgact gcgttcctgt aagtcctgcc
     2581 agcttgcgat agatttctgg cgcaccccct ttgagatgat caatgctcac cgtgggcgca
     2641 attttttcaa gttgttcaat gggcgtatta cgggtcggtt ctgtgatgat caggtcaggc
     2701 tttgccgcca caatcgcttc aatatcaata tcagcggtac caataaaggc gatcgatgaa
     2761 ttatcaaagt cgacacctgt caataacgcg ccagatcgaa tgaaatggct accgtcaggc
     2821 cgagttcgcc cgtggctggc gacaggaggt acaccgagct caatcagagg aatagtgata
     2881 tcaagatcgt gtaaagagac aattctcttt ggatgagcag gaacagtgac tttgcgccct
     2941 aaatcatcag taaatacctg aacaggttct gttgcacggg aaaaaaatga caacagtatc
     3001 agcagagaaa aaaataaacg catataactc ccgttaaaaa ctgttcctgc gctgccagag
     3061 cagtagcaaa aaaaatggtc caccaatcag tgagataatg attccggcag gcagttgcaa
     3121 aggcaaaaaa gccaaacgac caatgctatc cgccagaata actaacaacg cccccgatac
     3181 cgcgctcccc agcagtaatg tcgtctgccc gccacgtaga ataaaacgtg atatatgggg
     3241 tgctatcaat ccgacaaaac cgatgttacc aacacacgaa acgcagacgg ctgtcaggat
     3301 tattggtgcg gcgacacgta acaacgccag acgagaacta ttcaccccta acccggttgc
     3361 aacctgatga ccaagcagag cgatatccgc cgtcctggcg gtgaacagca ataaaagagc
     3421 ggctggcaac atccaacagg ctgaaatacc gactaacatc cagttagctg catgcaggct
     3481 tcccgataac cacatcaatg cagtttgtac atctcgaaca tctgcagtgg tcataaatac
     3541 accgataccg gcggcgaaaa accaggaaac accaatacca atcagaacaa atcgcgggcg
     3601 ggaaatatcg cgcgcacaaa aaatcacgat taaagccacc aacaagccac cagcaagacc
     3661 agccactgga cgccagaaca ttcccagcat tggaaactgg aaaatcagcc acagcaccgc
     3721 cacgctacat ccttctttga cgccgattaa accaggatcg gcaaggccgt tacgcgcaat
     3781 ggattgcatc gcggcccccg ccatacctaa catggctccg catagcaatg ccatgatgac
     3841 tctcggcaag cgaatatcct gcacaatata acgtgcgtct gtgcttacgt tttctggagc
     3901 aaacaacgct cgtcctactt ccgatgccgg gacagaaaaa gaaccatgca tcagaccgaa
     3961 tatcagtatt ccaaccgcaa acagaaacag cccaccaagc aacaccagat ttttagggcg
     4021 taccagtgtc gaaaacttac caacacgtag tgggcgtagc ccgacgttca ttatccgact
     4081 catttgaaca tcctcgtcgc cataatgaca aaaacaggcg caccaactaa cgccgtcatg
     4141 acaccggtcg ctaactcatg gggcgtgaaa agcgtccgag caataatatc tgccagtaat
     4201 aacaaaagcg cgccgacaca agctgaaaga gggagcagaa cgcgcagatc cgctgatacc
     4261 agacgccgga caatctgcgg tactagcagg ccgacaaaac caatggggcc agcaatcgat
     4321 acggctgcgc cacagagcaa cgcgatcgca agcagggtga atgttcgcgt gcggagtacc
     4381 gagacgccca gcccctgcgc catgcgatcg cccagtgcca acatgttaag agagggcgca
     4441 aggtaaatgg ctaacacaaa tccccccaga gaaaaccagg cggacgtacc cagcgtcacc
     4501 caatcctgcc ccgctaaatc acctgctagc catgtccgca tcgccagcag tgtttgttcg
     4561 tcaaaaatca ggattgcagc cgttattgag gacacgaacg ccgatagcgc cacgccgcat
     4621 aaagtaactt tcattggcgt taaccctgaa cgccctgcgg aggaaagcag caatatcaac
     4681 aggaacaata acgcgccccc cgtagatgcc agcagtgggc gtccaaccgg aaaggccagt
     4741 ccaagcgcac tggcggcaac gacagcaaga gcggctccgg catttaagcc taaaatatgt
     4801 ggctccccca atgggttacg aatgacagcc tgcaacaaag caccagcaac accaagagaa
     4861 gcaccggtaa gcaatgctgc cgctaaacgc ggcaaacgga gtcttacaat tacgttgtga
     4921 tcgaagtttt gcggatcaaa atgaagaaat gcctgcaaca ccgtttgtgg tgcaataaat
     4981 cgggcaccaa tccctggata taccacgcat cccaccagca gtaaggcgag gaaaattaca
     5041 gtaaccagcc ctgtgcgcat catacttttt ctacaccacg aaacggcatg aagaacggct
     5101 ttcccgtcag aggatttatt gatgcgtgaa catcaacgtc aaagacagct tttaccagtt
     5161 caggcgtgca atgctccccc tcattcaaca cacgcaccac tttcccctgg cgtaaaaata
     5221 tcaacgtatc gccataattg acggcaaaat tcagatcatg caacaccacg acgactgtac
     5281 gcccgtgatg acgcgttaaa tcatgtaata actcaagaat ttcaacctga taacgtaaat
     5341 caagccaggt tgtgggttca tccagcagga tataaggcgt cttttgcgcc aacaccatcg
     5401 caatccagca acgctggcgc tgcccaccag agagtttttc aacaggtaaa tgtgcaaact
     5461 cctgcgtacc ggttaatttc agagcctctt ctaccgcctg ctcgtcggcg tcgctccatt
     5521 gacgaatgaa attctgccag ggaaaacgcc cacgcgacac cagttcataa acggttaagc
     5581 cctccggcaa taatggcgac tgcggcaaga tcccaagtct acgggataac gctttcgttg
     5641 gttgctcgtg aatcgctttg ccatcaagca atatcgaacc gcccataggt tgcaagatac
     5701 gtgcaatggt gctgagcaac gtcgactttc cacagccgtt tgccccaacc agaacggtca
     5761 ttttttctgt gggaacggag aaagaaacac catcaacaat gatcttcttg tgatacccgg
     5821 cagaaacatt gtcgaggatc agcccctgcc cttttgcgtt atgtgccacg tgtacgccaa
     5881 cttaatcaaa gacaaacaaa aataaaactc attctccttt gagagtttga cgccacaaaa
     5941 tgtagtagtc aagcagttaa aagcc
//
LOCUS       DAZFLD010000239         5942 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.240, whole genome shotgun sequence.
ACCESSION   DAZFLD010000239 DAZFLD010000000
VERSION     DAZFLD010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5942
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.240"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            82..1215
                     /gene="ompN"
                     /locus_tag="ACHKBL_004251"
     CDS             82..1215
                     /gene="ompN"
                     /locus_tag="ACHKBL_004251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpN"
                     /protein_id="HFO5821031.1"
                     /translation="MKSKVLALLIPALLAAGAAHAAEVYNKDGNKLDLYGKVDGLHYF
                     SDNSAKDGDQSYARLGFKGETQINDQLTGYGQWEYNIQANNTESSTNQSWTRLAFAGL
                     KFADYGSFDYGRNYGVMYDIEGWTDMLPEFGGDSYTNADNFMTGRANGVATYRNTDFF
                     GLVNGLNFAVQYQGNNEGASNGQEGTNNGRDVRHENGDGWGLSTTYDLGMGFSAGAAY
                     TSSDRTNDQVNHTAAGGDKADAWTAGLKYDANNIYLATMYSETRNMTPFGDSDYAVAN
                     KTQNFEVTAQYQFDFGLRPAVSFLMSKGRDLHAAGGADNPAGVDDKDLVKYADVGATY
                     YFNKNMSTYVDYKINLLDEDDSFYAANGISTDDIVALGLVYQF"
     gene            1356..1790
                     /gene="uspF"
                     /locus_tag="ACHKBL_004252"
     CDS             1356..1790
                     /gene="uspF"
                     /locus_tag="ACHKBL_004252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspF"
                     /protein_id="HFO5821032.1"
                     /translation="MNRTILVPIDISDSELTQRVISHVEAEAKIDDAEVHFLTVIPSL
                     PYYASLGLAYSAELPAMDDLKAEAKSQLEEIIKKFKLPTDRVHVHVEEGSPKDRILEL
                     AKKIPAHMIIIASHRPDITTYLLGSNAAAVVRHAECSVLVVR"
     gene            1966..2901
                     /gene="ttcA"
                     /locus_tag="ACHKBL_004253"
     CDS             1966..2901
                     /gene="ttcA"
                     /locus_tag="ACHKBL_004253"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HFO5821033.1"
                     /translation="MSQNQEISKKEQYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMV
                     CLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQKQPGFPEHVLPEYLETLGVEYKI
                     VEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGATKIALGHHRDDILQTLFL
                     NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPN
                     LQRQVIADMLRDWDKRYPGRIETMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVNGGD
                     LAFDREEIPLQPAGWQPEEDENQLDELRLNVVEVK"
     gene            complement(3030..4403)
                     /gene="dbpA"
                     /locus_tag="ACHKBL_004254"
     CDS             complement(3030..4403)
                     /gene="dbpA"
                     /locus_tag="ACHKBL_004254"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HFO5821034.1"
                     /translation="MSAFSTLNVLPPAQLTNLNELGYLTMTPVQAAALPTILAGKDVR
                     VQAKTGSGKTAAFGLGLLQQIDASLFQTQALVLCPTRELADQVAGELRRLARFLPNTK
                     ILTLCGGQPFGMQRDSLQHAPHIIVATPGRMLDHLQKGTVSLDALNTLVMDEADRMLD
                     MGFSDAIDDVIRFAPASRQTLLFSATWPEAIAAISGRVQRDPLAIEIDSTDALPPIEQ
                     QFYETSSKGKIPLLQRLLSLHQPSSCVVFCNTKKDCQSVCDALNEVGQSALSLHGDLE
                     QRDRDQTLVRFANGSARVLVATDVAARGLDIKSLELVVNFELAWDPEVHVHRIGRTAR
                     AGNSGLAISFCAPEEAQRANIISDMLQIKLNWQTPPVNSSIVPLEAEMATLCIDGGKK
                     AKMRPGDVLGALTGDIGLDGADIGKIAVHPAHVYVAVRQAVAHKAWKQLQGGKIKGKT
                     CRVRLLK"
     gene            complement(4433..4606)
                     /gene="ynaL"
                     /locus_tag="ACHKBL_004255"
     CDS             complement(4433..4606)
                     /gene="ynaL"
                     /locus_tag="ACHKBL_004255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YnaL"
                     /protein_id="HFO5821035.1"
                     /translation="MTTLIYLQIPVPEPIPGDPVPVPDPIPRPQPMPDPPPDEEPIKL
                     SHRERRSARIRAC"
     gene            complement(4881..5864)
                     /gene="zntB"
                     /locus_tag="ACHKBL_004256"
     CDS             complement(4881..5864)
                     /gene="zntB"
                     /locus_tag="ACHKBL_004256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005385 - zinc ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0071577 - zinc ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HFO5821036.1"
                     /translation="MEAIKGADVNVPDAVFAWMLDGRGGVKPLENTDVIDEAHPCWLH
                     LNYVHHDSAQWLATTPLLPNNVRDALAGESTRPRVSRLGEGTLITLRCINGSTDERPD
                     QLVAMRVYMDGRLIVSTRQRKVLALDDVVSDLEEGTGPTDCGGWLVDVCDALTDHSSE
                     FIEQLHDKIIDLEDNLLDQQIPPRGFLALLRKQLIVMRRYMAPQRDVYARLASERLPW
                     MSDDQRRRMQDIADRLGRGLDEIDACIARTGVMADEIAQVMQENLARRTYTMSLMAMV
                     FLPSTFLTGLFGVNLGGIPGGGWQFGFSIFCILLVVLIGGVALWLHRSKWL"
     gene            complement(5878..5925)
                     /gene="ydaN"
                     /locus_tag="ACHKBL_004257"
     CDS             complement(5878..5925)
                     /gene="ydaN"
                     /locus_tag="ACHKBL_004257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YnaN"
                     /protein_id="HFO5821037.1"
                     /translation="MLRNRARRLDYKIMR"
BASE COUNT         1553 a         1509 c         1416 g         1464 t
ORIGIN      
        1 taactgaact tctcctttag gatgctttca ctccccaaaa tggggataaa aggcaaataa
       61 aataactaaa ggatttattc aatgaaaagc aaagtactgg cacttttaat tcctgccctg
      121 ctcgccgcag gtgctgcaca tgcagccgaa gtttataata aagacggcaa caaattagat
      181 ctgtatggca aagttgatgg cctgcattat ttttctgata attcagcgaa agatggcgac
      241 cagagctatg cgcgtctggg ttttaaaggc gaaacccaaa ttaacgatca actcactggc
      301 tacggtcaat gggaatacaa tattcaggca aacaacactg aatcttcaac aaaccagtca
      361 tggacccgtc tggcatttgc cggactgaaa tttgcagatt acggttcttt cgattacgga
      421 cgtaattatg gcgtaatgta cgacatcgaa ggctggaccg atatgctgcc tgaatttggc
      481 ggtgactctt ataccaatgc agacaacttt atgactggtc gtgccaacgg cgtcgcgact
      541 tatcgtaata ctgatttctt cggtctggta aatggtctga acttcgcggt gcagtatcaa
      601 ggtaacaacg aaggagccag taatggtcag gaaggcacca acaacggacg tgatgttcgc
      661 catgaaaacg gtgacggctg gggtctttcc acaacatatg atttaggcat gggctttagc
      721 gctggtgcgg catacacctc ttctgaccgc accaatgacc aggttaacca tactgcggcg
      781 ggtggtgata aagcagacgc gtggactgct gggctaaaat acgatgctaa caatatttac
      841 ctggcaacca tgtattcaga aacgcgtaac atgaccccgt ttggcgacag cgattatgca
      901 gttgcgaaca aaacccagaa ctttgaagtt actgcacagt accagtttga ttttggtctg
      961 cgtccggcag tctctttcct gatgtctaaa ggccgtgacc tgcacgctgc cggtggtgca
     1021 gacaacccgg caggtgttga tgataaagat ctggttaaat acgccgatgt tggcgcgact
     1081 tactatttca acaaaaatat gtccacctac gttgactata aaataaacct gttggatgaa
     1141 gatgacagct tctacgctgc caacggcatc tctaccgatg atattgtcgc tttaggtctg
     1201 gtttatcagt tctaaatcct cctgcccgct gttatggcgg gctttttctg cttattcttc
     1261 ctcctttgat ctaaattaaa aatgtgaact ccgtcattac acaaaaagtg tcatctggcg
     1321 ttacacttta tgcggatact aaaacaggag gttttatgaa cagaacgatt cttgtcccta
     1381 tcgatatttc cgattcagaa ttaactcaac gcgtgattag ccacgttgag gcagaggcaa
     1441 agattgatga tgcagaggtt catttcctga cggtaatacc ttcactgccc tactatgcct
     1501 ctctgggttt agcgtattcc gcagaattac cggcaatgga tgacctgaaa gcggaagcca
     1561 aatcgcaact ggaagagatc attaaaaaat ttaaactgcc aaccgacaga gtgcatgtcc
     1621 atgttgagga aggctcgccc aaagaccgca ttctggaatt ggcgaagaag atccccgctc
     1681 atatgatcat cattgcttcc catcgaccgg atatcaccac ttatctgctc ggttctaacg
     1741 ccgcagcggt agtgcgtcac gcagagtgct ccgtgctggt tgtgcgctga cattaacgcc
     1801 cgcacattgc tgcgggcttt ttgattcatt tcgcaaatgt gctgacattt tcccctctaa
     1861 tccgtaccat acacgccaca gtttttatat cagatttctt atgctgggcg ttccggcatg
     1921 acgcatactc ttctgatgcc atataacgaa ttgagtcgct tttaaatgtc gcaaaatcaa
     1981 gaaattagta agaaagaaca atacaacctg aacaaattac aaaaacgtct gcgtcgtaac
     2041 gtgggcgaag ccattgctga cttcaatatg attgaagaag gcgatcgcat catggtttgc
     2101 ctctccgggg gtaaagacag ctataccatg ctggaaattc tgcgcaattt gcagcaaagc
     2161 gcgccaatca atttttcgct ggtggctgtt aacctcgatc aaaagcaacc gggcttcccg
     2221 gaacacgttc tgcccgagta tcttgaaacg ctgggcgttg agtacaagat tgtggaagag
     2281 aacacttacg gtatcgtgaa agagaagatt ccggagggca aaaccacttg ctcactgtgt
     2341 tctcgtcttc gtcgcggtat cctttatcgc accgcaacgg aactgggggc gacgaagatc
     2401 gcgctgggtc accatcgtga cgatatcctg caaacgttgt tcttaaatat gttctacggc
     2461 ggcaagatga aaggtatgcc tccgaaactg atgagcgatg atggcaaaca tattgtgatt
     2521 cgtccgctgg cctactgccg cgagaaagat attcagcgct ttgccgatgc aaaagcgttc
     2581 ccgattattc cgtgcaacct gtgcggttca cagcctaacc tgcaacgtca ggtgattgct
     2641 gacatgttgc gtgactggga taaacgttat ccagggcgta tcgagacgat gttcagcgcg
     2701 atgcagaatg tggtgccgtc gcatctgtgc gataccaacc tgttcgattt caaaggtatt
     2761 acccacggtt ctgaagtggt taacgggggt gatctggcgt ttgatcgcga agagatccct
     2821 ctacaaccgg cgggttggca gcctgaagaa gatgaaaatc agttggatga gttacggctg
     2881 aatgtggttg aagtgaaata acccggatag cgcccgatgc gcaagcttac cgggctactc
     2941 ttatggaggc cggataagac gcggccagcg tcgcatccgg caatcccgaa gaagatgttt
     3001 actcttgcac cctgcgattc aacatttcat tattttaata accgcacccg gcacgttttt
     3061 cctttaatct tcccgccctg taactgtttc catgctttat gagcaacagc ctgacgcacc
     3121 gcgacataga catgcgccgg atgcacggcg attttgccaa tatctgcgcc atcaagcccg
     3181 atatctcctg tcagcgcacc taatacatca cccgggcgca ttttggcttt tttcccgcca
     3241 tcgatacata acgttgccat ttctgcttcc agcggcacaa tggaactatt aactggcggc
     3301 gtttgccagt taagttttat ctgcaacatg tcagaaatga tattggcccg ctgtgcttct
     3361 tccggggcac agaaactgat cgccagaccg ctatttcctg cacgagctgt acgaccgatg
     3421 cgatgtacat gaacttcagg gtcccacgcc agctcaaagt tcaccaccag ctcaagcgat
     3481 tttatatcca gaccacgcgc agcaacatca gtcgcgacca gtacacgggc gctaccgtta
     3541 gcaaaacgta ccagggtctg atcgcgatcg cgttgctcca gatcgccgtg taacgacaat
     3601 gcactttgcc ctacttcatt cagcgcgtcg cagacagact ggcaatcttt tttggtattg
     3661 caaaacacca cgcaagagga tggctgatgc aagcttaata accgttgcaa cagaggaatt
     3721 ttgcctttgc tggatgtctc ataaaattgt tgttcaatag gtggcagagc atctgttgag
     3781 tcaatttcaa tcgctaaagg atcgcgttgc actcttccgc tgattgcggc gatggcttcc
     3841 ggccaggttg ccgaaaacag aagcgtctgt cgagatgctg gcgcaaaacg gatgacatca
     3901 tcaatggcat cgctaaatcc catatccagc atgcggtcgg cctcatccat caccagcgta
     3961 ttcaacgcat ccagtgatac cgtgcctttt tgcagatgat ccagcatacg gcccggcgtt
     4021 gccacgataa tatgcggcgc atgttgcaac gaatcacgct gcataccgaa cggttgacca
     4081 ccgcacaacg tcaaaatttt ggtatttggc agaaaacgcg ccagccgacg caattcacct
     4141 gccacctgat ccgccagttc acgcgtagga cacagcacta aagcctgggt ttgaaatagc
     4201 gacgcatcaa tttgctgtaa caagccgagg ccaaaagccg ccgttttgcc gctgccggtt
     4261 ttcgcctgca cgcgaacatc ttttccggca aggatcgtcg gaagcgcggc ggcctgcacc
     4321 ggcgtcatgg ttaaataacc caactcatta aggttcgtga gttgggcggg aggcaaaaca
     4381 ttcagggtag aaaaagcgga cacaatttat tctcgtggtc atcgacgcaa agtcagcagg
     4441 cgcgtatcct cgcagatcta cgctcacgat gcgacaattt aatcggttct tcgtcgggtg
     4501 gtgggtcagg catgggttgc gggcgaggga tcggatcggg cactggaaca ggatcgccag
     4561 gaatcggttc agggacagga atttgcaaat aaataagtgt cgtcatattt ccctccggtc
     4621 attgggtgga ctcttaaagg gtagacgctg acaaataaca ggcaaaaaaa agccgactca
     4681 tcaaagtcgg cgtcgtacga atcaattgtg ctatgcagta attcaaaaaa ggaagtaaga
     4741 caatatggag cgcaacgccc atcgcttgac gttgcattca cctgcaagag agatattgcc
     4801 ctgaatgggt agagagttta ttgacttcgc tcaaactttg cggcgttttt gtatacagac
     4861 agccgggaaa attgcttttg ttacaaccat ttactacgat gcaaccataa agcaacacca
     4921 ccaataagaa caactaacag aatacaaaaa attgaaaatc cgaattgcca cccgccgcct
     4981 gggatcccac caaggttgac gccaaataac cctgtcagaa aggtactggg taaaaagacc
     5041 attgccatca acgacattgt ataggtacga cgcgctaaat tttcctgcat cacctgagcg
     5101 atttcatcag ccatcacgcc agtccgtgct atacaggcgt cgatttcgtc aaggccgcgc
     5161 ccaaggcgat cggcaatatc ctgcatacga cggcgttggt catcgctcat ccacggcaaa
     5221 cgttcactgg caagacgagc ataaacatca cgttgcggtg ccatatagcg acgcatcaca
     5281 attaattgtt tgcgcagcag agccaggaat ccacgcggtg gaatttgctg atcaaggaga
     5341 ttatcttcaa ggtcgataat tttatcgtgc agctgctcga taaattcgct ggaatgatcg
     5401 gtcaacgcat cacacacatc caccagccat cccccgcaat cggtcggacc ggtgccttct
     5461 tccagatcgc ttaccacatc gtccagcgcc agcactttgc gttgtcgggt cgaaacaatt
     5521 aaccgcccgt ccatatatac acgcatggcg accagttgat cggggcgttc atcggtgctg
     5581 ccgtttatac agcgcaatgt aatcagcgta ccttcaccga gacggctgac tcggggccgt
     5641 gtgctctcgc ccgccagcgc atcacgtacg ttattgggaa gcagcggtgt tgtcgccagc
     5701 cattgggcgc tatcatggtg tacataatta aggtggagcc agcaggggtg cgcttcatca
     5761 atcacatctg tattttccag cggtttaacg ccgcctctac catccagcat ccaggcaaat
     5821 actgcatccg ggacattaac gtccgctccc ttaatcgctt ccacagtgcc tccatcatca
     5881 acgcattatt ttgtagtcta gccttctggc cctgttacgc aacatctcat caccccatta
     5941 cc
//
LOCUS       DAZFLD010000240         5890 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.241, whole genome shotgun sequence.
ACCESSION   DAZFLD010000240 DAZFLD010000000
VERSION     DAZFLD010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5890
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.241"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            82..338
                     /locus_tag="ACHKBL_004258"
                     /pseudo
     CDS             82..338
                     /locus_tag="ACHKBL_004258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001632353.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(360..631)
                     /locus_tag="ACHKBL_004259"
                     /pseudo
     CDS             complement(360..631)
                     /locus_tag="ACHKBL_004259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000206187.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(628..2388)
                     /locus_tag="ACHKBL_004260"
     CDS             complement(628..2388)
                     /locus_tag="ACHKBL_004260"
                     /EC_number="2.1.1.-"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha/beta hydrolase/class I
                     SAM-dependent methyltransferase"
                     /protein_id="HFO5821038.1"
                     /translation="MTNSRIPDERQFLTSDNTELFYRHWPAQEASSTPKVVVLFHRGH
                     EHSGRLQHIVDELAMPDTAFYAMDARGHGRSPGLRGYSPSLVRSVQDVDEFVRFAAAD
                     SQAGMTDVVVVAQSVGAVLAATWAHDYAPDIRGLVLASPAFKVKLYVPFARQGLELMY
                     RLRGLFFVNSYVKGKYLTHDPERVASFNKDPRITRPIAVNILLDLYKTAERVVSDASA
                     ITLPVQLLVSGEDYVVHRQPQLDFYQNLRNPQKELHVLPGFYHDTLGEKERHLAFDRM
                     RSFIDTLYARPVQRFDYSQEDRWSPSADTWRILSGGPALYSLDDISYRCLRYGMKVLG
                     TQSTGVRLGFETGFDSGSTLDYIYRNQPQGSTSLGRLIDKNYLNSVGWKGIRQRKIHL
                     QMLIQQAVARLTEQGSSVRIVDIAAGHGRYVLDALQGETAVSEILLRDYSELNVAKGQ
                     EMIASRGMAGRARFEQGDAFNRNELAALEPRPTLGIVSGLYELFPENGPVRDSLAGLA
                     AAIEPGGILIYTCQPWHPQLKTIAWSLTSHKNGKAWVMRVRTQGEMDALVRDAGFEKC
                     TQLIDEMGIFTVSMAVRRQP"
     gene            complement(2381..3022)
                     /locus_tag="ACHKBL_004261"
     CDS             complement(2381..3022)
                     /locus_tag="ACHKBL_004261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HFO5821039.1"
                     /translation="MMTRLLRTLFTLCIVWPVIWLWLGLRVRHRERLPKHGPSIIVAN
                     HNSHMDVFALLSLFPLRYQASVHPVAAADYFLRDKRMAWFALKILNIIPISRKGGYTD
                     PLAQCEQALREGKTLILFPEGTRGEPGVLAPLKSGVWHLSQRMPEVPIIPVGLRGTER
                     VMAKGNRIPLPLFIDVNIGDALSTHPEKKTFMDDLEQRLTALLQPSQGNHPHD"
     gene            complement(3026..3943)
                     /locus_tag="ACHKBL_004262"
     CDS             complement(3026..3943)
                     /locus_tag="ACHKBL_004262"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016156609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFO5821040.1"
                     /translation="MTLLEKSLIVIFSLLLIATVINGLLVWLRPSKDWRELTLRIRTW
                     WVIIVLFSFALLSPSWLALTFFALVSFMALKEFLTLVPSRQSDRMPLLWMFIAIPLNY
                     WLIGIGWYGMFVVFIPVYVFLFLPARMVIAGDTQGFLRTVSQLHWSLMTTVFAFSHVA
                     FLLVLPADGMQTSALLVLFLVGLTEFNDIAQYLWGKSLGRIKVIPKVSPNKTLEGLLG
                     GVATTALAATVLGPLLTPLSWPMALLAGVIIGVTGFCGDVVMSAIKRDIGVKDSGTLL
                     PGHGGILDRLDSLIFTAPVFFHFVRYFCY"
     gene            complement(3940..4545)
                     /locus_tag="ACHKBL_004263"
     CDS             complement(3940..4545)
                     /locus_tag="ACHKBL_004263"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015730493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFO5821041.1"
                     /translation="MTLYQIKPSFQALLRPLMFWLNRRGVTANHITLCAMVLSLITGV
                     VLIIHPQPGLFVLLPVVLFVRMALNALDGMLARECNQRSRLGAILNETGDVISDISLY
                     LPFMLLPQSSIPLVLIVVFCALMTEFCGVLAQTINGVRSYAGPLGKSDRALVFGAWGL
                     SLSIWPQIVAWNNVIWGVATLLLLWTIFNRCRSALREEGVK"
     gene            complement(4603..4788)
                     /locus_tag="ACHKBL_004264"
     CDS             complement(4603..4788)
                     /locus_tag="ACHKBL_004264"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5821042.1"
                     /translation="MLTQLHRVLYRCPGHYVYGSATQSTGTGTGSLASRNTSQSPLPA
                     QQAGFPRPELYSLSVCQ"
     gene            complement(5029..>5890)
                     /locus_tag="ACHKBL_004265"
     CDS             complement(5029..>5890)
                     /locus_tag="ACHKBL_004265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFO5821043.1"
                     /translation="KGIMFAFLAAFSWGAAIVMSKKGLENMDAGELFLWQVGSAAMLS
                     WFVLAISRKKLPVTKKSILAYSTGIFEPFLAYTFTLYGLKFISAGITAVIFSLESVFI
                     LILSVILFSVKINSPLHFILLLTGAMAGSIMVALPDINDRIDNITGYILIVAGVLSAS
                     FYVVISSRLIVNFEPVTLLTGQLTFSFILACFFIVLTGTSLQLPIESVFLVFLSGILQ
                     YFLAFCFYLYSLRWIPVHIAGAMLYFIPLVALLLSWFFLQEDISFIQGLGVFITILCV
                     YILNKKYAIE"
BASE COUNT         1643 a         1537 c         1405 g         1305 t
ORIGIN      
        1 ctatctctgc ctttgcttca tgggatgttc tttgtatatt ttacttgctg tttaagtatg
       61 tatataagga gtgcattcat tatgccaatg aagcttgtaa agaagaatcc ccggagcaaa
      121 gtgattcggg agcttagctg tgagcaagag atggcagtaa aggcggtctg cggactgaaa
      181 aaacagcaac tacggcacta catgccttgg ccaatgatct tctccgggaa atgcgtgagt
      241 atgatgtatg gttacagtgt gactgtattc cgggagattc gccgaccatg aattttgctg
      301 ctcttaaaaa caacacggga actttttatc tgtccagttt ggatatagat aattattgta
      361 ctaataaggg aagccaacac cagccgacag accagacgcc gctgctcatt cagtgaaacg
      421 cagataaaca gtaaagcaaa tccagactcc agtacggaat aatcgtcagc ggtattaacg
      481 ggatccggtg ctcccagtca aacaccaggc tgctgatgtt ttctctcccg gcggtaaact
      541 gattcgtcat gccataagta agaaagaaga atggggccag caccattagc cagccgattc
      601 cctgaataat caccgcacgc cgcgttgtca tggttgacgt cgtaccgcca tagagaccgt
      661 gaagatgccc atttcatcaa ttagttgcgt gcatttctca aaaccggcgt cacggaccag
      721 cgcgtccatc tcgccctgag tacgtacgcg cattacccag gctttgccat ttttgtgact
      781 ggtgagtgac caggcgatgg tttttaactg agggtgccag ggctgacagg tgtagatcag
      841 aatgcctccc ggttcaatcg ctgcagccag cccggcaagc gaatctcgta ccggcccgtt
      901 ttccgggaac agttcataca gaccggacac gatccccagc gtcggacgcg gttccagtgc
      961 cgccagttca ttacgattaa aggcatcccc ctgctcaaat cgcgcacggc ccgccatacc
     1021 gcgagaggct atcatctcct gacctttcgc cacgtttaat tcgctgtaat cgcgcagaag
     1081 gatctcgctt accgccgttt cgccttgcag ggcatcaagc acatagcgtc catgcccggc
     1141 ggcaatatcc acgatccgga ccgagctacc ctgctctgtt aaccgagcaa cagcctgctg
     1201 aattaacatc tgcagatgaa ttttacgctg gcggatcccc ttccagccca cgctattcag
     1261 gtagttttta tcaatgaggc gtcccaacga ggtgctgccc tgaggctggt tgcgatagat
     1321 gtagtctagg gtactgccgg aatcgaaacc agtctcaaat cccagacgca cccccgtgga
     1381 ctgcgtgccc agcactttca tcccgtagcg caaacagcgg taagagatat cgtccagcga
     1441 atacagcgca gggccaccgc tcagtatgcg ccaggtatcg gcgctggggc tccagcgatc
     1501 ttcctggctg taatcaaaac gctgcacggg acgggcatac aaggtatcaa taaaactgcg
     1561 catcctgtca aatgccagat gccgctcttt ttcgcctaag gtgtcatgat aaaatcccgg
     1621 cagtacatgc agttctttct gcggatttcg caggttctga taaaaatcaa gctgcggctg
     1681 gcggtgcaca acgtaatctt ccccggagac cagcaactgc acaggtagcg taatggcgct
     1741 ggcatcgctg acgactcgct cagccgtttt gtagagatca agcagaatat tcacagcaat
     1801 tggtcgggta atccgcggat ccttattgaa gcttgctacc cgctcgggat cgtgcgtcag
     1861 atacttgcct ttcacatacg agttgacgaa gaaaagaccg cgcaggcgat acatcaattc
     1921 cagcccctgg cgagcaaacg gcacatacaa tttgacctta aacgccggtg atgccaacac
     1981 caggccacga atgtccgggg cataatcatg cgcccaggtt gccgccagaa ccgccccgac
     2041 gctttgcgcc acgacgacca catccgtcat cccagcttgg ctatctgcgg cggcgaaacg
     2101 cacaaactcg tcaacatcct gcaccgaacg cacgagcgac ggactgtaac cacgcagacc
     2161 cggtgagcga ccatgacctc gggcatccat ggcataaaac gctgtatccg gcatcgccag
     2221 ctcatcaaca atgtgttgca aacgcccgga gtgctcatgc ccacgatgaa acaggaccac
     2281 cacttttggc gttgaactgg cttcttgtgc tggccagtgg cgataaaaca gctctgtatt
     2341 atcactggtc agaaactgcc gttcatcggg tatgcgggaa ttagtcatgg ggatgatttc
     2401 cttgcgaggg ttgcaaaagc gccgttagtc tttgctccag gtcgtccata aacgttttct
     2461 tctccggatg cgtacttagc gcatcgccaa tatttacatc aataaacagc ggcaaaggga
     2521 tacgattacc tttcgccatc acacgctcag taccgcgcaa cccaacgggg ataatgggca
     2581 cctccggcat gcgctgactc aaatgccaca ctccggattt caatggcgca agtacgcctg
     2641 gctcaccgcg cgtcccttcc ggaaacagga tcagtgtttt accttcacgc agcgcctgtt
     2701 cacattgcgc cagcggatcg gtataaccgc cctttcttga aataggaatg atgttaagga
     2761 tcttcagcgc aaaccaagcc atgcgtttat cccgcagaaa ataatccgcg gcagcaaccg
     2821 gatgaacaga agcctggtaa cgcagcggaa aaagcgaaag caaggcgaaa acatccatat
     2881 ggctgttgtg atttgccacg attatcgaag gcccgtgctt cggcagtcgc tcgcggtggc
     2941 ggacacgcag accaagccac agccagatga ccggccagac gatacacagc gtaaacaggg
     3001 tacggagtag tcgtgtcatc ataggttagt agcagaaata gcgaacaaaa tggaagaaga
     3061 ccggtgcggt aaaaatcagc gaatccagcc gatcaaggat gcctccatgg ccgggtagca
     3121 gcgtaccact gtctttgacg ccgatatcac gcttgattgc cgacatcacc acgtcgccgc
     3181 aaaagccagt aacgccgata ataaccccgg ctaacagtgc catcggccag ctcaggggag
     3241 tcaacagtgg ccccaggact gttgccgcca gcgccgtcgt tgccacccca cccagcaatc
     3301 cctccagggt tttgtttggg ctgaccttcg gtatgacttt tatacgacca agcgacttgc
     3361 cccacaggta ttgcgcgatg tcgttaaatt cggtcaggcc gaccaaaaac agcaccaaca
     3421 gcgcgctggt ttgcattcca tccgccggta acaccagtaa aaacgcgaca tggctgaagg
     3481 caaatacggt ggtcatcaga ctccagtgca actgggaaac ggtacgtaaa aagccctgtg
     3541 tatccccagc aatcaccatt cgcgccggta aaaaaaggaa cacgtagacc gggataaaca
     3601 ccacaaacat gccgtaccag ccgataccta tcagccagta gttaagggga attgcaataa
     3661 acatccataa cagcggcatg cggtcggact ggcgggaagg caccagcgtc aaaaactctt
     3721 ttagcgccat aaagctgacc agcgcgaaga acgtcagtgc cagccagcta ggacttaata
     3781 gtgcgaagga aaacaggacg ataatcaccc accaggtgcg aatacgtaat gtcagctcgc
     3841 gccagtcttt acttggacgc agccacacca gcaacccgtt gattacggtg gcaatcaaca
     3901 gcagggaaaa aataacgatc agtgattttt caagtagcgt catttcacac cctcttcacg
     3961 caatgcgctg cgacagcgat taaaaatcgt ccataacaac aacagtgttg cgacacccca
     4021 tatgacatta ttccaggcga caatctgcgg ccagatcgac agtgacagcc cccatgcacc
     4081 aaacaccagc gctctgtcgc ttttgcccag cggaccggca tagctgcgca cgccgttaat
     4141 cgtttgcgcc agcacgccgc aaaactcggt catcagcgca caaaacacca cgatcagcac
     4201 cagcggtata ctgctctgcg ggagtagcat aaagggcaga taaagcgaga tgtcggaaat
     4261 tacatcaccc gtctcattga ggatggcccc cagacgcgat ctttgattac attcacgcgc
     4321 cagcatacca tcgagcgcat tgagcgccat tcgtacaaac aacaccacgg gcagaaggac
     4381 gaataatccg ggctgaggat gaataatgag caccacgccg gtgataaggg aaagcaccat
     4441 cgcgcacagt gtaatgtgat tagcggtgac gccgcgcctg ttcagccaga acatcagcgg
     4501 tcttaacaga gcctgaaacg agggctttat ctggtaaagg gtcatcatca ctccttaata
     4561 agcatatttg acggggttga gtctggcctt ttattggggc tgtcactggc acactgaaag
     4621 tgaatacagt tccgggcgcg gaaatcctgc ctgctgcgcc ggtaaaggcg actgactggt
     4681 attacgggag gccagagagc cagtacctgt gccggtagac tgtgtggctg aaccataaac
     4741 atagtgtcct ggacaacggt acagcactct gtgcagctgt gtcagcatgc gcttttcttt
     4801 aattatgccc agtcatgtgc ggaggcaagg atttcaaaat atccgttaca tttgttgcat
     4861 gatgccttat cgctggtccg ggctattgct ttacttcttt taaatcagct ctgatgaaca
     4921 ttttttacca ctttatcgca ttaaggttat gtcattcatc gtctggcatc acgtttttgg
     4981 ttatggtgtc agggtgtacc ctaccgcgat aacgcaaccc taataaagtc attcgatggc
     5041 atatttctta ttgagaatat atacgcataa aatagttata aaaacgccta gtccctgaat
     5101 aaatgatata tcttcctgca ggaagaacca ggacaacagc aatgctacca gaggaataaa
     5161 atagagcatt gctcctgcta tgtgaacggg aatccagcgt agggaataaa gatagaaaca
     5221 aaaagccaaa aaatattgta aaattcctga caaaaacaca agaaatacag attctattgg
     5281 cagttgcagg gatgtgccag ttagaacaat aaaaaaacaa gcaagaataa aagagaaagt
     5341 caactgcccg gttaacaacg taacaggctc aaaattcaca ataagtcgtg atgagataac
     5401 aacataaaag gatgctgaga gtacacctgc aacaataagt atatatcctg taatattatc
     5461 aatgcggtca ttgatatcag gtagggcaac cattatactg cctgccattg ctccagtaag
     5521 caataatata aagtgtaatg gtgagttaat tttaacggaa aataatataa cagatagtat
     5581 aagaataaat actgactcaa gtgaaaaaat aacagctgtt ataccagcag aaataaattt
     5641 taggccatag agagtgaatg tatacgctag aaatggctca aaaattcctg ttgaataggc
     5701 aaggattgat ttttttgtta ccgggagttt ttttctgcta attgcgagaa caaaccatga
     5761 taacattgca gcactaccaa cttgccataa aaacaactct ccggcatcca tgttttcgag
     5821 accttttttg ctcatcacta ttgctgctcc ccatgaaaaa gcagcaagaa atgcaaacat
     5881 aattccctta
//
LOCUS       DAZFLD010000241         5877 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.242, whole genome shotgun sequence.
ACCESSION   DAZFLD010000241 DAZFLD010000000
VERSION     DAZFLD010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5877
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.242"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            1..296
                     /locus_tag="ACHKBL_004266"
                     /pseudo
     CDS             1..296
                     /locus_tag="ACHKBL_004266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
     gene            complement(495..1619)
                     /gene="yhhJ"
                     /locus_tag="ACHKBL_004267"
     CDS             complement(495..1619)
                     /gene="yhhJ"
                     /locus_tag="ACHKBL_004267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO5821044.1"
                     /translation="MRHLRNIFNLGIKELRSLLGDKAMLTLIVFSFTVSVYSSATVTP
                     GSLNLAPIAIADMDQSQLSNRIVNSFYRPWFLPPEMITADEMDAGLDAGRYTFAINIP
                     PNFQRDVLAGRQPDIQVNVDATRMSQAFTGNGYIQNIINGEVNSFVARYRDNSEPLVS
                     LETRMRFNPNLDPAWFGGVMAIINNITMLAIVLTGSALIREREHGTVEHLLVMPITPF
                     EIMMAKVWSMGLVVLVVSGLSLVLMVKGVLGVPIEGSIPLFMLGVALSLFATTSIGIF
                     MGTIARSMPQLGLLVILVLLPLQMLSGGSTPRESMPQMVQDIMLTMPTTHFVSLAQAI
                     LYRGAGFEIVWPQFLTLMAIGGAFFTIALLRFRKTIGTMA"
     gene            complement(1619..4354)
                     /gene="rbbA"
                     /locus_tag="ACHKBL_004268"
     CDS             complement(1619..4354)
                     /gene="rbbA"
                     /locus_tag="ACHKBL_004268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HFO5821045.1"
                     /translation="MTHLELVPVPPVAQLAGVSQHYGKTVALNNITLDIPARCMVGLI
                     GPDGVGKSSLLSLISGARVIEQGSVMVLGGDMRDPKHRRDVCPRIAWMPQGLGKNLYH
                     TLSVYENVDFFARLFGHDKAEREVRINELLTSTGLAPFRDRPAGKLSGGMKQKLGLCC
                     ALIHDPELLILDEPTTGVDPLSRAQFWDLIDSIRQRQSNMSVLVATAYMEEAERFDWL
                     VAMNAGEVLATGSAEELRQQTQSATLEEAFINLLPQAQRQAHQAVVIPPYHPENAEIA
                     IEARDLTMRFGSFVAVDHVNFRIPRGEIFGFLGSNGCGKSTTMKMLTGLLPASEGEAW
                     LFGQPVDPKDIDTRRRVGYMSQAFSLYNELTVRQNLELHARLFHIPEAEIPARVAEMS
                     ERFKLNDVEDVLPESLPLGIRQRLSLAVAVIHRPEMLILDEPTSGVDPVARDMFWQLM
                     VDLSRQDKVTIFISTHFMNEAERCDRISLMHAGKVLASGTPQELVEKRGAASLEEAFI
                     ACLQEAAGQSNEAEAPPVIHDTTHAARQGFSLRRLFSYSRREALELRRDPVRSTLALM
                     GTVILMLIMGYGISMDVENLRFAVLDRDQTVSSQAWTLNLSGSRYFIEQPPLTSYDEL
                     DRRMRAGDITVAIEIPPNFGRDIARGTPVELGVWIDGAMPSRAETVKGYVQAMHQSWL
                     QDVASRQSTPASQSGLMNIETRYRYNPDVKSLPAIVPAVIPLLLMMVPSMLSALSVVR
                     EKELGSIINLYVTPTTRSEFLLGKQLPYIALGMLNFFLLCALSVFVFGVPHKGSFLTL
                     TLAALLYIIIATGMGLLISTFMKSQIAAIFGTAIITLIPATQFSGMIDPVASLEGPGR
                     WIGEVYPTSHFLTIARGTFSKALDLTDLWQLFIPLLIAIPLVMGLSILLLKKQEG"
     gene            complement(4351..5418)
                     /gene="yhiI"
                     /locus_tag="ACHKBL_004269"
     CDS             complement(4351..5418)
                     /gene="yhiI"
                     /locus_tag="ACHKBL_004269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFO5821046.1"
                     /translation="MDKSKRHLAWWVVGALAVAAVVAWWLLRPAGVPEGFAVSNGRIE
                     ATEVDIASKIAGRIDTILVKEGQFVREGEVLAKMDTRVLQEQRLEAIAQIKEAQSAVA
                     AAQALLEQRQSETRAAQSLVNQRQAELDSVAKRHTRSRSLAQRGAISAQQLDDDRAAA
                     ESARAALESAKAQVSASKAAIEAARTNIIQAQTRVEAAQATERRIAADIDDSELKAPR
                     DGRVQYRVAEPGEVLAAGGRVLNMVDLSDVYMTFFLPTEQAGTLKLGGEARLILDAAP
                     DLRIPATISFVASVAQFTPKTVETSDERLKLMFRVKARIPPELLQQHLEYVKTGLPGV
                     AWVRVNEELPWPDDLVVRLPQ"
     gene            complement(5481..5594)
                     /gene="yhiY"
                     /locus_tag="ACHKBL_004270"
     CDS             complement(5481..5594)
                     /gene="yhiY"
                     /locus_tag="ACHKBL_004270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhiY"
                     /protein_id="HFO5821047.1"
                     /translation="MMTTLLPVFTKPSPLALNALCAGRICHFLLIPDGRIR"
BASE COUNT         1369 a         1667 c         1522 g         1319 t
ORIGIN      
        1 tgactatttg tgccgttatt tctggtgcag aaggttggga agatatagag gattttgggg
       61 aaactcatct cgattttttg aagcaatatg gtgattttga aaatggtatt cctgttcacg
      121 ataccattgc cagagttgta tcctgtatca gtcctgaaaa atttcacgag tgctttatta
      181 actggatgcg tgactgccat tcttcagatg ataaagacgt cattgtaatt gatggaaaaa
      241 cgctccggca ctcttatgac aagagtcgcc gcaggggagc gattcatgtc atttctcgcg
      301 tcagtccttg cggggagcgg gctttcgtaa tcttgccctg aataccggac tgagtataaa
      361 aaagccggtt aactgaaact gtccaggttt tggtggtcag ttcataaacg ccttatccgg
      421 cctaaaaaaa actaaaattc aataaattgc accaatgcgt agaccctata agcttacgca
      481 tcgggcaact gtaattacgc cattgtccca attgtcttcc tgaatctcag cagtgcgatg
      541 gtgaaaaacg cgccgccaat tgccatcagt gtcaggaact gcggccagac gatttcgaat
      601 ccggcaccac ggtagagaat ggcctgcgcg aggctaacaa agtgtgtcgt cggcatggtc
      661 agcataatgt cctgcaccat ctgcggcata ctttcgcgcg gcgtggaacc accggaaagc
      721 atttgcagcg gcagcagcac cagaatcacc agcagcccca gttgcggcat tgaacgcgct
      781 atcgtcccca taaaaatacc gattgacgtg gtggcaaaca gactgagcgc cacgcccagc
      841 ataaacagcg ggatcgagcc ttctatcggt acgcccagca cgccttttac catcagcacc
      901 agcgataatc ccgacaccac cagcaccacc agccccatcg accagacctt tgccatcatg
      961 atctcaaacg gcgttatcgg catcaccagt aagtgttcca ccgtgccgtg ttcacgctcg
     1021 cggatcagcg ccgatccggt caatacaatc gccagcatgg taatgttgtt gatgatcgcc
     1081 atcaccccgc caaaccacgc gggatcaagg ttcgggttaa agcgcatccg tgtttccagc
     1141 gataccaacg gttcgctgtt atcacggtag cgcgcgacaa agctgttcac ttcaccgttg
     1201 ataatattct ggatataccc attgccggta aatgcctggc tcatgcgcgt ggcatcgacg
     1261 ttcacctgaa tatccggctg gcgtccggcg aggacatcac gctgaaaatt aggcggaata
     1321 tttatcgcga aggtataacg tccggcgtcc agcccggcat ccatctcatc ggcggtgatc
     1381 atctccggtg gcaaaaacca cgggcgatag aagctgttaa cgatccggtt cgataactgc
     1441 gattgatcca tatcggcaat ggcgatcggc gcgaggttca acgatcctgg cgtaacggtc
     1501 gctgacgaat acaccgacac cgtaaacgag aagacaatca gcgtcagcat cgctttatca
     1561 ccgagcagac tgcgcaactc tttgataccc agattaaaaa tattacgtaa atggcgcatc
     1621 atccctcctg ttttttcagc agcaggatgc ttaagcccat caccagcggt atggctatca
     1681 gcaacgggat aaaaagttgc cacaaatcag tcagatccag cgctttcgag aacgttccgc
     1741 gggcgatagt cagaaaatga ctggtcgggt aaacctcgcc gatccaacgt cctggacctt
     1801 ccagcgaagc taccggatcg atcatcccgg aaaactgtgt cgccgggatc aacgtgataa
     1861 ttgccgtacc aaaaatggcg gcaatctggc ttttcataaa ggtggagatc agcagcccca
     1921 ttccggtggc gatgatgata tacagcagcg ccgccagggt gagagtcagg aaactgcctt
     1981 tatgcggtac gccaaataca aataccgaca gggcgcagag caggaaaaag ttcagcatcc
     2041 ccagcgcgat gtatggcagc tgtttgccaa gcaaaaattc gctacgggtg gtgggggtca
     2101 cgtaaaggtt gataatcgac cctagctctt tttcccgcac cacgctaagg gcgcttagca
     2161 ttgacgggac catcatcagc agaagcggga tcaccgccgg aacaatcgcc ggcaggcttt
     2221 ttacgtccgg gttatagcga tagcgcgtct caatattcat cagcccgctt tggctggcgg
     2281 gtgtggactg tcggctcgcc acatcctgta gccagctctg gtgcatggcc tgcacgtagc
     2341 cttttaccgt ttcggcgcgg ctcggcatcg caccgtcgat ccagacaccg agttccacgg
     2401 gcgtaccacg cgcgatatcg cgcccgaaat taggagggat ctcaattgcc accgtgatat
     2461 cgcccgcacg catccgacga tcaagctcgt cataactggt gagcggcggc tgttcgataa
     2521 agtaacggga accggagaga ttgagcgtcc acgcctggct actgacggtt tgatcgcggt
     2581 caagcaccgc aaagcgcagg ttttccacat ccatactgat gccgtaaccc attatcagca
     2641 tcaggatcac cgttcccatc agcgccagcg tcgaacgtac cggatcgcgt cgcagttcca
     2701 gcgcttcgcg gcggctgtag ctaaacagac ggcgcaggct aaatccctga cgcgccgcgt
     2761 gggtggtgtc gtgtatcacg ggcggcgctt cggcttcgtt gctctgccct gccgcttcct
     2821 gcaaacaggc gataaatgcc tcttccagac ttgcggctcc gcgtttctca accagttcct
     2881 gcggtgtccc gctggcgagc acttttccgg cgtgcatcag tgagatacgg tcgcaacgtt
     2941 ccgcttcgtt cataaagtgg gtggagatga agatagtcac tttgtcctgg cgcgagagat
     3001 cgaccatcaa ctgccagaac atatccctcg ccaccggatc gacgccagaa gtaggctcat
     3061 cgaggattaa catctccggg cgatgaatca ccgccaccgc cagcgaaagc cgctggcgaa
     3121 tgccgagcgg caatgactcc ggcagaacat cttcaacgtc gttgagctta aaacgctcgc
     3181 tcatttcagc cactcgcgcg ggaatttccg cttccgggat gtgaaacaaa cgggcatgta
     3241 actcaaggtt ttgccgcacg gtgagttcgt tatagagcga aaacgcctgc gacatatagc
     3301 ctacccgacg gcgggtatcg atatcttttg gatcaaccgg ttgcccaaac agccacgcct
     3361 caccttcgct ggcgggcagc agtccggtga gcattttcat ggtggtggat ttaccgcagc
     3421 cgttcgaacc gaggaatcca aaaatctccc cgcgtggaat gcggaaattg acgtgatcaa
     3481 cggcaacgaa ggaaccaaaa cgcatggtca gatcgcgcgc ttcgatggca atctctgcgt
     3541 tttcagggtg atacggtggg atcactaccg cctgatgcgc ctggcgttgc gcttgcggta
     3601 acagatttat aaatgcttct tccagcgtgg cgctttgcgt ttgctgccgc agctcttcgg
     3661 cgctgccggt tgccagcact tctccggcat tcatcgctac cagccagtcg aagcgttcgg
     3721 cctcttccat gtaggcggtg gcgaccagca cgctcatatt gctctgccgc tggcgaatgc
     3781 tgtcgatcaa atcccagaac tgggcgcggg agagcgggtc aacccccgtt gttggctcat
     3841 caagaatcaa cagttccggg tcgtggatta acgcacagca cagcccaagt ttttgcttca
     3901 tcccgccgga gagtttccct gccggacgat cacgaaacgg tgctaacccg gtgctggtca
     3961 gcagctcatt gattcgcact tcccgctccg ctttgtcgtg accgaacagg cgagcgaaaa
     4021 aatcgacgtt ttcatacacc gacaaggtgt ggtagaggtt tttgcccagc ccctgcggca
     4081 tccaggcgat gcgcgggcaa acgtcgcggc gatgcttcgg gtcgcgcata tcgccgccca
     4141 gcaccatcac gctgccctgc tcaatgacgc gggcaccgga aatcaacgac aacaagctcg
     4201 atttaccgac accatccggt ccaatcagcc cgaccatgca acgggccgga atatcgagtg
     4261 tgatattgtt cagcgcaacg gtttttccat aatgctggct cacgcccgcc agttgcgcga
     4321 caggcgggac gggaaccagt tccagatgcg tcattgcggc aacctcacca ccaggtcgtc
     4381 aggccacgga agttcttcat tcacccgcac ccacgctacg cccggcaatc cggttttgac
     4441 atattccaga tgctgctgga gtaattccgg tgggatacgc gctttgacac ggaacatcaa
     4501 tttcagccgc tcatcgctgg tttcgacggt ttttggcgtg aactgggcga cgctggcgac
     4561 aaaactgatg gtcgcgggaa tacgcagatc tggtgcagca tcaagaatca aacgtgcttc
     4621 accgcccagt ttcagcgtgc ccgcctgttc ggttggcaga aagaaggtca tatagacgtc
     4681 gctgagatcg accatattca gcacccgacc gcctgccgcc agcacttcgc ctggctcggc
     4741 aacccgatac tgcacgcgtc cgtcacgcgg ggctttcagt tcgctgtcat cgatatctgc
     4801 ggcaatgcgc cgttcagtgg cttgtgccgc ttcgacgcga gtttgcgcct gaatgatatt
     4861 ggtgcgtgcc gcctctatag cggctttaga agcagaaacc tgagcttttg ccgattccag
     4921 cgcagctcgg gcgctctcag cggcggcgcg gtcgtcatcc aactgttgcg cagaaattgc
     4981 cccccgttgt gccagtgagc gggaacgcgt atgacgtttt gctacggagt ccagttctgc
     5041 ctggcgttga ttaaccagcg actgtgcggc acgagtttcg ctttggcgtt gctccagcaa
     5101 agcctgcgcg gcggcaacgg cactttgtgc ttctttgatt tgcgcgatgg cttccagtcg
     5161 ctgttcctgc aacacgcgag tatccatctt cgccagcact tcaccttctc gaacaaactg
     5221 gccttctttc accagaatgg tgtcgatacg cccggcaatt ttgctggcga tatccacttc
     5281 cgtcgcttca atgcgcccat tgctgacagc aaagccttcc ggcacacccg ccgggcgcaa
     5341 cagccaccac gccacgacgg cagccaccgc cagtgccccg acaacccacc acgccagatg
     5401 gcgcttactc ttatccataa tcgatccgcc ataatccctg taagcaatcg acgcgtgtta
     5461 caactgccac cgaacgatgt tcagcggatt cttccatccg ggataagaag gaaatggcaa
     5521 atacgaccag cgcacagagc attgagcgcc aatggggaag gcttagtgaa aacgggtagt
     5581 aatgttgtca tcatcagcat tcctggccgt aaatgaaaca cgcatactca taagtgaaat
     5641 caattaaata ctaacagtta tattcatgtt gtctatttga ttgccatcaa cttgtgatac
     5701 atcgcgatgt tcttccgttc gctggtgttt tttccaaaaa tattctgtac aggttcacag
     5761 attctgttgc acaggtttcc gtaacaggga aacctgatga ctatcacata gcgttattat
     5821 cgcctgatgt gcgaataaat accgaatata aaataattta ttctgaaaag atgatag
//
LOCUS       DAZFLD010000242         5823 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.243, whole genome shotgun sequence.
ACCESSION   DAZFLD010000242 DAZFLD010000000
VERSION     DAZFLD010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5823
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.243"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            38..1027
                     /gene="ldhA"
                     /locus_tag="ACHKBL_004271"
     CDS             38..1027
                     /gene="ldhA"
                     /locus_tag="ACHKBL_004271"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HFO5821048.1"
                     /translation="MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANG
                     CEAVCIFVNDDGSRPVLEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDP
                     EAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
                     RILKGFGMRLLAFDPYPSAAALELGVEYVDLPTLFSESDVISLHCPLTPENYHLLNEA
                     AFEQMKNGVMIVNTSRGALIDSQAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVI
                     QDDVFRRLSACHNVLFTGHQAFLTAEALTSISQTTLQNLSNLEKGETCPNELV"
     gene            1138..1560
                     /gene="hslJ"
                     /locus_tag="ACHKBL_004272"
     CDS             1138..1560
                     /gene="hslJ"
                     /locus_tag="ACHKBL_004272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HslJ"
                     /protein_id="HFO5821049.1"
                     /translation="MKKVAALVALSLLMAGCVSNDKIAVTPEQLQHHRFVLESVNGKP
                     VTSDKNPPEISFGEKMMISGSMCNRFSGEGKLSNGELTAKGLAMTRMMCANPQLNELD
                     NTISEMLKEGAQVDLTANQLTLATAKQTLTYKLADLMN"
     gene            complement(1557..1823)
                     /locus_tag="ACHKBL_004273"
     CDS             complement(1557..1823)
                     /locus_tag="ACHKBL_004273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HFO5821050.1"
                     /translation="MRAAFWVGCAALLLSACSSEPVQQATAAHVAPGLKASMSSSGEA
                     NCAMIGGSLSVARQLDGTTIGMCALPNGKRCSEQSLAAGSCGSY"
     gene            2097..5621
                     /gene="nifJ"
                     /locus_tag="ACHKBL_004274"
     CDS             2097..5621
                     /gene="nifJ"
                     /locus_tag="ACHKBL_004274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HFO5821051.1"
                     /translation="MITIDGNGAVASVAFRTSEVIAIYPITPSSTMAEQADAWAGNGL
                     KNVWGDTPRVVEMQSEAGAIATVHGALQTGALSTSFTSSQGLLLMIPTLYKLAGELTP
                     FVLHVAARTVATHALSIFGDHSDVMAVRQTGCAMLCAANVQEAQDFALISHIATLKSR
                     VPFIHFFDGFRTSHEINKIVPLADDTILDLMPQAEIDAHRARALNPEHPVIRGTSANP
                     DTYFQSREATNPWYNAVYDHVEQAMNDFAAATGRQYQPFEYYGHPQAERVIILMGSAI
                     GTCEEVVDELLTRGEKVGVLKVRLYRPFSAKHLLQALPDSVRTVAVLDRTKEPGAQAE
                     PLYLDVMTALAEAFNNGERETLPRVIGGRYGLSSKEFGPDCVLAVFAELNAAKPKARF
                     TVGIYDDVTNLSLPLPENTLPNSAKLEALFYGLGSDGSVSATKNNIKIIGNSTPWYAQ
                     GYFVYDSKKAGGLTVSHLRVSEQPIRSAYLISQADFVGCHQLQFIDKYQMAERLKPGG
                     IFLLNTPYSVDEVWSRLPQEVQAVLNQKKARFYVINAAKIARECGLAARINTVMQMAF
                     FHLTQILPGDSALAELQGAIAKSYSSKGQDLVERNWQALALARESVEEVPLQPVNPHS
                     ANRPPVVSDAAPDFVKTVTAAMLAGLGDALPVSALPPDGTWPMGTTRWEKRNIAEEIP
                     IWKEELCTQCNHCVAACPHSAIRAKVVPPEAMENAPASLHSLDVKSRDMRGQKYVLQV
                     APEDCTGCNLCVEVCPAKDRQNPEIKAINMMSRLEHVEEEKINYDFFLNLPEIDRSKL
                     ERIDIRTSQLITPLFEYSGACSGCGETPYIKLLTQLYGDRMLIANATGCSSIYGGNLP
                     STPYTTDANGRGPAWANSLFEDNAEFGLGFRLTVDQHRVRVLRLLDQFADKIPAELLT
                     ALKSDATPEVRREQVAALRQQLKDVAEAHELLRDADALVEKSIWLIGGDGWAYDIGFG
                     GLDHVLSLTENVNILVLDTQCYSNTGGQASKATPLGAVTKFGEHGKRKARKDLGVSMM
                     MYGHVYVAQISLGAQLNQTVKAIQEAEAYPGPSLIIAYSPCEEHGYDLALSHDQMRQL
                     TATGFWPLYRFDPRRADEGKLPLALDSRPPSEALEETLLHEQRFRRLNSQQPEVAEQL
                     WKDAAADLQKRYDFLAQMAGKAEKSNTD"
BASE COUNT         1458 a         1489 c         1503 g         1373 t
ORIGIN      
        1 agcttaaatg tgattcaaca tcactggaga aagtcttatg aaactcgccg tttatagcac
       61 aaaacagtac gacaagaagt acctgcaaca ggtgaacgag tcctttggct ttgagctgga
      121 attttttgac tttctgctga cggaaaaaac cgctaaaacc gccaatggct gcgaagcggt
      181 atgtattttc gtaaacgatg acggcagccg cccggtgctg gaagagctga aaaagcacgg
      241 cgttaaatat atcgccctgc gctgtgccgg tttcaataac gtcgaccttg acgcggcaaa
      301 agaactgggg ctgaaagtag tccgtgttcc agcctatgat ccagaagccg ttgctgaaca
      361 cgccatcggt atgatgatga cgctgaaccg ccgtattcac cgcgcgtatc agcgtacccg
      421 tgacgccaac ttctctctgg aaggtctgac cggctttact atgtatggca aaacggcagg
      481 cgttatcggt accggcaaaa tcggtgtggc gatgctgcgc attctgaaag gttttggtat
      541 gcgtctgctg gcgttcgatc cgtatccaag tgcggcggcg ctggaactcg gtgtggagta
      601 tgtcgatctg ccaaccctgt tctctgaatc agacgttatc tctctgcact gcccgctgac
      661 accggaaaac tatcatctgt tgaacgaagc tgccttcgaa cagatgaaaa atggcgtgat
      721 gatcgtcaat accagtcgcg gtgcattgat tgattctcag gcagcaattg aagcgctgaa
      781 aaatcagaaa attggttcgt tgggtatgga cgtgtatgag aacgaacgcg atctgttctt
      841 tgaagataaa tccaacgacg taattcagga tgacgtattc cgtcgcctgt ctgcctgcca
      901 caacgtgctg tttaccgggc atcaggcatt cctgacagca gaagcgctga ccagtatttc
      961 tcagactacg ctgcaaaact taagcaatct ggaaaaaggc gaaacctgcc cgaacgaact
     1021 ggtttaatct tgccgctccc ctgcattcca ggggagctga ttcagataat ccccaatgac
     1081 ctttcatcct ctattcttaa aatagtcctg agtcagaaac tgtaattgag aaccacaatg
     1141 aagaaagtag ccgcgctcgt tgcgctaagc ctgctgatgg cgggatgtgt aagtaatgac
     1201 aaaattgctg taaccccaga acagctacag catcatcgct ttgtgctgga aagcgtaaac
     1261 ggtaagcccg tgaccagcga taaaaatccg ccagaaatca gctttggtga aaagatgatg
     1321 atttccggca gcatgtgtaa ccgctttagc ggtgaaggca aactgtctaa tggtgaactg
     1381 accgccaaag ggctggcaat gacccgtatg atgtgcgcta acccgcagct taatgaactc
     1441 gataacacca ttagcgaaat gctgaaagaa ggtgcacaag tggatctgac cgcgaaccag
     1501 ttaacgctgg cgaccgcgaa acagacatta acttataagc tggcggattt aatgaattaa
     1561 tagctgccac agctcccggc ggcaagtgac tgttcgctac agcgtttgcc gttgggtaat
     1621 gcacacatcc caatcgtcgt accatccagt tgacgggcaa cagaaagcga accgccgatc
     1681 attgcacaat ttgcttctcc actactggac atcgacgctt ttaaacctgg cgctacgtgc
     1741 gccgcagtcg cctgctgaac aggttcacta ctacacgccg acaacaataa agcggcacac
     1801 cctacccaaa acgctgctcg catcttttct tcctctgatc ttcaagccaa acgacaccgc
     1861 cataaataat aggcagcaca gagggcggcg tcgagagctg tcctgagcgt tgccccgcat
     1921 ttttactttt ttatggctat ttttttgccc tctgtttgat caaaacattc attacgctga
     1981 tgtgggggac acaaaagcga aaatgcagaa gaaagccatt tgctaaaatt gaaggattac
     2041 tactgggcgc gcagcaattt cgtgcgcccc tcatttgcgc aatgtaaggg tgtcatatga
     2101 ttactattga cggtaatggc gcggttgctt cggtggcgtt tcgcaccagt gaagttatcg
     2161 ccatctaccc tattaccccc agttccacga tggcagaaca ggctgatgcc tgggccggaa
     2221 acggcttgaa gaacgtttgg ggagacacac cacgcgtggt tgaaatgcag tcggaagcgg
     2281 gtgctatcgc taccgtgcat ggcgctttgc agacgggtgc cctctcaaca tcgtttacgt
     2341 catcgcaggg tttgctgctg atgatcccga cgctgtacaa actggcaggc gaactgacgc
     2401 catttgtcct gcatgtagcg gcacgtaccg ttgccacaca tgcactctct atttttggcg
     2461 atcattccga cgttatggcg gtgcgccaga cgggttgcgc gatgttgtgt gcggctaacg
     2521 tccaggaagc gcaagacttt gctctcattt cgcacatcgc aacgctgaag agccgcgtgc
     2581 catttattca tttctttgat ggtttccgca cgtcccacga aatcaataaa attgtcccgc
     2641 tggccgatga cactattctt gatctcatgc cgcaggccga aattgatgct catcgcgccc
     2701 gggcgctcaa cccggaacat ccggtgatcc gcggtacgtc cgccaatcct gacacttatt
     2761 tccagtctcg cgaagccacc aacccatggt acaacgcagt ctatgaccat gttgaacagg
     2821 cgatgaatga tttcgctgcc gcgacaggtc gccagtatca gccgtttgag tattacgggc
     2881 atccacaagc ggaacgggtg attatcctga tgggctctgc cattggcacc tgtgaagaag
     2941 tggttgatga attgctgacc cgtggcgaaa aagtcggcgt gctgaaagtt cgcctgtacc
     3001 gccccttctc cgccaaacac ttactgcaag cgttgccgga ctctgttcgg acagtggcgg
     3061 tgctggatcg caccaaagag ccaggtgcgc aagcggaacc cctgtacctg gatgtgatga
     3121 ccgcactggc agaagccttt aataatggcg agcgtgaaac cctgccccgt gtcattggtg
     3181 ggcgctatgg tctttcatcc aaagaatttg gcccggactg tgtactggcg gtatttgccg
     3241 agctcaacgc ggctaaaccg aaagcgcgct ttacggtagg tatttacgat gatgtgacca
     3301 atctgtcact gccgttgccg gaaaacaccc tgccaaactc ggcgaaactt gaagccttgt
     3361 tttatggcct tggtagtgat ggcagcgttt ccgcgaccaa aaacaatatc aagattatcg
     3421 gtaattccac gccgtggtac gcacagggct attttgttta cgactccaaa aaggcgggcg
     3481 gcctgacggt ttctcacctt cgcgtgagcg agcagccgat tcgttccgct tatctgattt
     3541 cccaggctga ttttgttggc tgccaccagt tgcagtttat cgataaatat cagatggctg
     3601 aacgtttaaa acctggcggc attttcctgc tcaacacgcc gtacagcgta gatgaagtat
     3661 ggtcgcgctt gccgcaggaa gttcaggccg tgttaaacca gaaaaaagcg cgcttctatg
     3721 tgattaacgc ggcgaaaatc gcccgcgaat gtggtctggc ggcccgtatt aataccgtca
     3781 tgcagatggc ttttttccat ctgacgcaaa ttctgcctgg cgatagcgcc ctcgcagaat
     3841 tgcagggtgc gattgccaaa agttacagta gcaaaggtca ggatctggtg gagcgcaact
     3901 ggcaggctct ggcgctggcg cgtgaatccg tagaagaagt tccgttgcag ccggttaatc
     3961 cgcacagcgc caatcgaccg cctgtggttt ccgatgccgc tcctgatttc gtgaaaaccg
     4021 ttaccgcggc gatgctcgcc gggcttggtg atgccctccc cgtttcggcg ctgccgccag
     4081 acggcacctg gccgatgggc accacgcgct gggaaaaacg caatatcgcc gaagagatcc
     4141 ccatctggaa agaggaactc tgtacccaat gtaaccactg cgtcgctgct tgcccacatt
     4201 cggctattcg cgcaaaagtg gtaccgcctg aagcgatgga aaacgcccct gccagcctgc
     4261 attcgctgga tgtgaaatcg cgtgatatgc gcgggcagaa atatgtcttg caggtggcac
     4321 cggaagattg caccggctgt aacctgtgcg tcgaagtttg cccggcgaaa gaccgtcaga
     4381 atccagagat taaagccatc aatatgatgt cgcgcctgga acatgtcgaa gaagagaaaa
     4441 tcaattacga tttcttcctc aacctcccag aaatcgaccg tagcaaactg gaacgtattg
     4501 atattcgtac atcacagctg attacaccgc tgtttgaata ttcaggtgct tgctccggtt
     4561 gtggcgagac gccgtatatt aaattgctga ctcagctcta tggcgaccga atgttgatcg
     4621 cgaacgccac cggctgttct tcaatttatg gcggtaacct gccctctaca ccgtatacta
     4681 ccgatgccaa cggtcgtgga ccggcatggg cgaactcgct gtttgaagat aacgccgaat
     4741 ttggccttgg tttccgcctg acggtcgatc aacaccgtgt ccgcgtgttg cgtctgctgg
     4801 atcaatttgc cgataaaatc ccggcggaat tactaactgc gttgaaatca gacgccacgc
     4861 cagaggttcg tcgtgaacag gttgcagctt tacgccagca actcaaagat gttgccgaag
     4921 cacatgaact gctacgtgac gcagatgcac tggtagaaaa atcaatctgg ctgattggtg
     4981 gtgatggctg ggcttacgat atcggctttg gcggtctgga tcatgtattg agtttgacgg
     5041 aaaacgtcaa cattctggtg ctggatacgc aatgctattc caacaccggt ggtcaggcgt
     5101 cgaaagcgac accgctgggt gcagtaacta agttcggcga gcacggcaaa cgtaaagcgc
     5161 gtaaagatct tggcgtcagt atgatgatgt acggtcatgt ttatgtggcg cagatttctc
     5221 tcggcgcgca gctcaaccag acggtgaaag cgattcagga agcggaagcg tatccggggc
     5281 catcgctgat catcgcttat agcccctgtg aagagcatgg ttacgatctg gcactcagtc
     5341 acgaccagat gcgccaactc actgccaccg gcttctggcc gctatatcgc ttcgatccac
     5401 gtcgagccga tgaaggcaaa ctgccgctgg cattggattc acgcccgccg tcagaagcac
     5461 tggaagaaac gttacttcac gagcagcgtt tccgtcgtct gaattcgcag cagccagaag
     5521 tggcagaaca gttatggaaa gatgctgcag ctgatttgca aaaacgctat gacttcctgg
     5581 cacaaatggc cggaaaagcg gaaaaaagca acaccgatta aatgcactgg ataaggatta
     5641 tccaattcta aaaaaaagcc cggacgactg ttcgggcttg tctttttata tgttggaaaa
     5701 tcagtagcaa tgcaatggcc caacagaaaa taaaatctgt gacaataaag gcatataacc
     5761 cgcgcagaat aacgtatagc aaattaaaag tataattttt attttgtatg tattatcttc
     5821 aat
//
LOCUS       DAZFLD010000243         5816 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.244, whole genome shotgun sequence.
ACCESSION   DAZFLD010000243 DAZFLD010000000
VERSION     DAZFLD010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5816
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.244"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(332..1291)
                     /gene="nrdF"
                     /locus_tag="ACHKBL_004275"
     CDS             complement(332..1291)
                     /gene="nrdF"
                     /locus_tag="ACHKBL_004275"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFO5821052.1"
                     /translation="MKLSRISAINWNKITDDKDLEVWNRLTSNFWLPEKVPLSNDIPA
                     WQTLSVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMPDALTPHEEAVLSNISFMEAVHA
                     RSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
                     LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKNLEKISQARREEL
                     KSFAFDLLLELYDNELQYTDELYAETPWADDVKAFLCYNANKALMNLGYEPLFPAEMA
                     EVNPAILAALSPNADENHDFFSGSGSSYVMGKAVETEDEDWNF"
     gene            complement(1301..3445)
                     /gene="nrdE"
                     /locus_tag="ACHKBL_004276"
     CDS             complement(1301..3445)
                     /gene="nrdE"
                     /locus_tag="ACHKBL_004276"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFO5821053.1"
                     /translation="MATTTAERLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVDAF
                     MTTHVRPNSVAFSSQQQRLNWLVNEGYYDESVLNRYSSDFVITLFAHAHASGFRFQTF
                     LGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDETLATQLTDEMLSGRFQPA
                     TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAG
                     APIKRIENQSSGVIPVMKMLEDAFSYANQLGARQGAGAVYLHAHHPDILRFLDTKREN
                     ADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDIERVYGKPFADVAISEHYAEL
                     VANERIRKKYINARDFFQRLAEIQFESGYPYIMYEDTVNRANPIAGRINMSNLCSEIL
                     QVNSASEYDENLDYARTGHDISCNLGSLNIAHTMDSPDFARTVETAVRGLTAVSDMSH
                     IRSVPSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDFTNLYFYTITWHALRT
                     SMLLARERGETFAGFKQSRYASGEYFSQYLQSNWQPKTAKVGELFARSSITLPTREMW
                     AQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPA
                     PFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIY
                     AWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL"
     gene            complement(3418..3828)
                     /gene="nrdI"
                     /locus_tag="ACHKBL_004277"
     CDS             complement(3418..3828)
                     /gene="nrdI"
                     /locus_tag="ACHKBL_004277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HFO5821054.1"
                     /translation="MSQIVYFSSSSENTQRFIERLGLPAVRIPLNERERIQVDEPYIL
                     IVPSYGGGGTAGAVPRQVIRFLNDEHNRALLRGVIASGNRNFGEAYGRAGDVIARKCG
                     VPWLYRFELMGTQSDIENVRKGVTEFWQRQPQNA"
     gene            complement(3825..4070)
                     /gene="nrdH"
                     /locus_tag="ACHKBL_004278"
     CDS             complement(3825..4070)
                     /gene="nrdH"
                     /locus_tag="ACHKBL_004278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HFO5821055.1"
                     /translation="MRITIYTRNDCVQCHATKRAMENRGFDFEMINVDRVPEAAEALR
                     AQGFRQLPVVIAGDLSWSGFRPDMINRLHPAPHAANA"
     gene            complement(4317..4646)
                     /locus_tag="ACHKBL_004279"
     CDS             complement(4317..4646)
                     /locus_tag="ACHKBL_004279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HFO5821056.1"
                     /translation="MFNRPNRNDVDDGVQDIQNDVNQLADSLESVLKSWGSDAKGEAE
                     AARSKAQALLKETRARMHGRTRVQQAARDAVGCADSFVRERPWCSVGTAAAVGIFIGA
                     LLSMRKS"
     gene            4798..5142
                     /locus_tag="ACHKBL_004280"
     CDS             4798..5142
                     /locus_tag="ACHKBL_004280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2002 family protein"
                     /protein_id="HFO5821057.1"
                     /translation="MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARM
                     GRTALVIHPTLKERSSTLAEPASDIKTCDHYQQFPLYLAGERHEHYGIPHGFSSRVAL
                     ERYLNGLFGEAS"
     gene            complement(5179..5628)
                     /gene="alaE"
                     /locus_tag="ACHKBL_004281"
     CDS             complement(5179..5628)
                     /gene="alaE"
                     /locus_tag="ACHKBL_004281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HFO5821058.1"
                     /translation="MFSPQSRLRHAVADTFAMVVYCSVVNMCIEVFLSGMSFEQSFYS
                     RLVAIPVNILIAWPYGMYRDLFMRAARKVSPSGWIKNLADIMAYVTFQSPVYVAILLV
                     VGADWHQIMAAVSSNIVVSMLMGAVYGYFLDYCRRLFKVSRYQQVKA"
BASE COUNT         1467 a         1416 c         1449 g         1484 t
ORIGIN      
        1 gtttatgtcg atacattacc caatggaaat agtcactttt ttctacccta acatactgag
       61 aatctgaggc aacccctgat ggcaaatgtg gcatgaatat tgatgtgagc aaatcgccgg
      121 aatttcccat gatataaggg ctgagagcaa atcgagaaaa gttgatattt tttgtcaaag
      181 atagcaaaaa aacgcctgaa tattgtggtt tcaggcgaat aatgcaacag agtatcagtg
      241 tagatcacca caaattattt gcgtgatata aagctatttg gcgggaatta tttccctgca
      301 gcgggtagtg atatttttga aaataacccc ctcagaaatt ccagtcttca tcttctgttt
      361 cgaccgcttt tcccatcaca tatgaagagc ctgaaccgga aaagaaatcg tgattttcat
      421 cggcattcgg cgaaagcgcg gcgaggattg ccggattcac ttccgccatt tctgcgggaa
      481 atagcggctc gtagcccaga ttcatcaacg ccttattggc gttgtaacag agaaacgctt
      541 tcacatcgtc agcccacggg gtttcggcgt acagctcatc ggtgtattgc aactcgttgt
      601 catagagttc cagcaacaaa tcgaaggcga aactcttcaa ttcttcacgt cgtgcctggg
      661 agatcttttc caggtttttc tgatatttat agccgatgta gtaaccgtgg actgcttcat
      721 cgcggataat cagacggatc aggtctgcgg tattggttag ctttccgcgg ctggaaaaat
      781 acatcggcag ccagaaaccg gaatagaaca aaaaagattc aagaaacaca ctggcgattt
      841 tctttttcag cggatcatca ccgcgataat gttgctgaat aatctgtgct tttcgctgca
      901 acggcgcgtt ttcttcactc caggcgtagg cggcatcgac atctttggtc tggcacagcg
      961 tcgagaaaat cgaactgtaa gagcgggcat gaaccgcctc cataaagctg atattcgata
     1021 atactgcctc ttcatgaggc gtgagcgcat cgggcatcag cgaaggcgcg ccgataacat
     1081 tttgcagcgt gtcgagcagt gtcaggccag taaaaacgcg catcgtcagt tgttgttcta
     1141 cgacacttaa tgtctgccag gcgggaatat cgttcgatag tggcaccttt tctggtagcc
     1201 agaaattgct ggtcaggcgg ttccacacct ccagatcttt atcgtctgtt atcttgttcc
     1261 agttgatggc gctgatacgt gagagtttca tagatattcc ttaaagtgca caggagacgc
     1321 agccttcaat ttcagtgcct tccagcgcca tctgacgcag gcggatgtag tagagcgttt
     1381 tgataccttt gcgccaggcg taaatctgcg ctttgttgat atcgcgagtt gtggcggtat
     1441 cggggaaaaa cagtgtcagc gacagcccct gatcgacatg gcgagtcgct tcggcgtagg
     1501 tgtcgatgat cttttctgcg cctatttcgt aagcgtcctg atacagcgcc agattctcgt
     1561 tagtcataaa cggggcagga tagtaaacgc gtcctgtttt gccctctttg cgtatctcta
     1621 ctttcgccac aatcggatga atactcgacg tggcatggtt gatataagag atagaaccgg
     1681 ttggcggcac cgcctggaga ttttggttgt atatgccata gcgcatcacg tcgtcgcgca
     1741 gttgcgccca catttcgcgg gtaggtaacg tgatgctgct acgagcaaac aactcgccaa
     1801 ctttcgccgt tttcggttgc cagttacttt gcagatattg gctaaaatat tcaccgctgg
     1861 catagcgcga ctgtttgaac ccggcaaagg tttcgccgcg ttcgcgcgcc agcaacatcg
     1921 aggtacgcag tgcgtgccag gtgatggtat agaaatagag attggtgaaa tcgagtgctt
     1981 ccggcgatcc ataagcgatg ccttctcgcg ccagataacc gtgtaaattc atctgcccca
     2041 gtccgatggc gtgtgaggcg gcatttccgg cttcgatgga cggcacgctg cggatatgac
     2101 tcatatctga caccgccgtt aaaccgcgca cggcagtctc taccgtgcgg gcaaagtcgg
     2161 gggaatccat ggtgtgggca atattcaacg aacctaaatt gcaggaaata tcatggcctg
     2221 tgcgggcata gtcgagattc tcgtcatact ctgaggcgct gttaacctgc aaaatttctg
     2281 agcagagatt gctcatattt atgcgcccgg cgatagggtt agcacggtta accgtgtctt
     2341 catacatgat gtacggatag ccggactcaa actggatttc tgccagtcgc tggaagaaat
     2401 cacgggcgtt gatgtatttt ttgcggatgc gttcattggc aaccagttcg gcatagtgtt
     2461 cactgatggc gacatcggca aatggcttgc cataaactcg ctctatgtca tagggcgaaa
     2521 acagcgccat ctgcgcattc tcttttgcca gatggaaagt gatatccggg atcaccacgc
     2581 ccagcgacag tgttttaatg cggatttttt cgtcggcatt ttcccgtttt gtatcgagaa
     2641 aacgcagaat atcgggatga tgagcatgta aatagactgc gccagcaccc tgacgcgcgc
     2701 cgagctggtt ggcataggaa aatgcgtctt ccagcatttt catcaccgga atcacgccag
     2761 aagattgatt ttcaatacgt ttaattggcg cgcccgcttc ccgcagattc gacagcaaaa
     2821 atgcaacgcc gccgccgcgt ttcgacagtt gcagtgcgga atttactgcc cggccaatcg
     2881 actccatatt gtcttcaata cgcagcaaaa aacaggaaac cagttcgccg cgctgctgct
     2941 taccacagtt gaggaatgtc ggtgttgcag gctgaaagcg tcctgacagc atctcatcgg
     3001 tcagttgcgt cgccagtgtt tcatcgcctt gtgccagcgt cagcgccacc atcgtgactc
     3061 gatcggcaaa atcttccaga taacgcttac cgtcgaatgt cttcaacgta tagctggtgt
     3121 aaaacttcca tgctccgagg aatgtctgaa aacgaaaacc gctggcgtgt gcgtgggcaa
     3181 acagcgtaat gacaaaatcg ctagagtagc gattaaggac actttcatca tagtaacctt
     3241 cgttaaccag ccagttcagg cgctgctgct ggctactgaa ggccacactg ttcggacgca
     3301 catgcgtcgt cataaaggcg tctactgcct ggcgatcttt atcgaactga atgcgacctg
     3361 cgctatcgta aaggttaagc atcgcattca gcgcgtggta atccatagtt tcctgcgtca
     3421 ggcgttctgc ggttgtcgtt gccaaaattc ggttactcct ttacgaacgt tttcgatatc
     3481 gctttgcgta cccatgagtt caaagcggta cagccatggc acgccgcatt tccgggcaat
     3541 cacatctccg gcgcggccat atgcctcacc aaagttgcga ttacccgaag caataacgcc
     3601 gcgcagcaac gcccggttat gctcgtcgtt taaaaagcga atgacctgtc gtggcaccgc
     3661 gccagccgta ccgccgccgc cgtaagaggg cacgatcagg atgtaaggct cgtctacctg
     3721 aatccgttcc cgctcattga gcgggatgcg caccgcgggc agccctaaac gttcgataaa
     3781 acgctgcgta ttttcggagc tgctggaaaa gtagacaatc tggctcatgc attggccgcg
     3841 tgtggtgctg gatgcagacg gttgatcatg tccgggcgga aacctgacca gctaagatcg
     3901 cctgcaatca cgaccggcaa ctgacgaaag ccctgagcac gcagcgcttc cgccgcttca
     3961 ggaacgcgat cgacattaat catttcaaag tcaaagcccc ggttttccat cgcccttttg
     4021 gtggcgtggc actgaacgca atcgttacga gtgtaaatag taatgcgcat gattcgtatt
     4081 tccgtttaaa atgaagatac ggcgcgatga tacgcgtcgg gttgtctctc tgttgataca
     4141 gaaatactag atgtagttga aaaaagattc aaccacacaa tatatagcaa atagtggtcg
     4201 aaattaccct gggtatgagc gtgacggggt agggggattt ttgtgattca ccaggcaaaa
     4261 agaaaccccg aagccaggct tcggggttaa agacgcgtat ttattatcat gttgcactac
     4321 gatttgcgca tgcttaacag tgcgccgata aaaataccta ccgcagctgc tgtacccacg
     4381 ctacaccagg gtctttcacg aacaaaagaa tccgcgcagc caacggcatc gcgcgcggct
     4441 tgctggacgc gagtacgacc atgcattcgc gctctggttt ctttcagtag agcctgcgct
     4501 ttactacgag cggcttcggc ttcccctttg gcgtcgctgc cccaggactt caatacagat
     4561 tcgagactat cagctaattg attgacatca ttctggatat cctgcacgcc atcatcaacg
     4621 tcgttgcggt tcggtcggtt aaacatatga tcccccatta gaattcgatg taattttcag
     4681 tgtagaccat aattttctta actgaggttc atcgcagctt tatggacttt tctgaaagcg
     4741 ttgtgaatgc gcaatcaggt aaggtaggcc cgcggttgat aataacgagg atcaaatatg
     4801 tatttacgac cagacgaggt ggcgcgcgta cttgaaaaag tcggttttac tgtcgatgtg
     4861 gtaacccaga aggcttatgg ttaccgccgt ggggaaaatt atgtctatgt taatcgcgaa
     4921 gcgcgaatgg gacgtaccgc cctggtgatt catccaacat taaaagaacg tagttcaaca
     4981 ctggctgaac ccgcttccga tattaaaact tgcgatcatt atcaacaatt tccgctctat
     5041 ttagcaggcg agcgacacga gcattacggt atcccgcatg gctttagttc gcgtgttgcg
     5101 cttgaacgtt atttgaatgg tttatttggc gaagccagtt aaagacgcga ctggcgatgc
     5161 cagtcgcgaa aagaagggtc aggcttttac ctgctggtaa cggctgactt taaacagtcg
     5221 gcggcaataa tcgaggaagt agccataaac cgcccccatc aacatcgaaa caacgatgtt
     5281 tgaactgacc gccgccataa tctgatgcca gtctgcgccc accactaaca agatcgccac
     5341 atacaccggt gactggaacg tcacataagc catgatatcc gccagatttt ttatccagcc
     5401 cgacgggcta actttgcgtg ctgcgcgcat aaacagatcg cggtacatac cgtatggcca
     5461 tgcaattaag atgtttaccg gaatcgctac caatctggaa taaaaagact gttcgaagct
     5521 cattccggag aggaaaactt caatacacat gttcacgaca gaacagtaaa caaccatcgc
     5581 gaacgtatct gcaactgcat gacgcaagcg tgactgcggt gagaacatgc taatgctcct
     5641 taataaaagc gtaatgggat gtgaatggag atgaaagcag tggatctcat tgccatctgc
     5701 atttttgcat agcgtatatc gctggctatg actaatcaat tagtgaaaaa tttttattta
     5761 atctgttttt gtagataaaa tattccgtag cggcgcgttt tttgtacatt gtggat
//
LOCUS       DAZFLD010000244         5771 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.245, whole genome shotgun sequence.
ACCESSION   DAZFLD010000244 DAZFLD010000000
VERSION     DAZFLD010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5771
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.245"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(86..430)
                     /gene="yoaB"
                     /locus_tag="ACHKBL_004282"
     CDS             complement(86..430)
                     /gene="yoaB"
                     /locus_tag="ACHKBL_004282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFO5821059.1"
                     /translation="MTIVRIDAEARWSDVVIHNNTLYYTGVPENLDADAFEQTANTLA
                     QIDAVLEKQGSNKSSILDATIFLADKNDFAAMNKAWDAWVVAGHAPVRCTVQAGLMNP
                     KYKVEIKIVAAV"
     gene            562..2472
                     /gene="yoaA"
                     /locus_tag="ACHKBL_004283"
     CDS             562..2472
                     /gene="yoaA"
                     /locus_tag="ACHKBL_004283"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HFO5821060.1"
                     /translation="MTDDFAPDGQLAKAIPGFKPREPQRQMAVAVTQAIEKGQPLVVE
                     AGTGTGKTYAYLAPALRAKKKVIISTGSKALQDQLYSRDLPTVSKALKYTGNVALLKG
                     RSNYLCLERLEQQALAGGDLPVQILSDVILLRSWSNQTVDGDISTCVSVAEDSQAWPL
                     VTSTNDNCLGSDCPMYKDCFVVKARKKAMDADVVVVNHHLFLADMVVKESGFGELIPE
                     ADVMIFDEAHQLPDIASQYFGQSLSSRQLLDLAKDITIAYRTELKDTQQLQKCADRLA
                     QSAQDFRLQLGEPGYRGNLRELLANPQIQRAFLLLDDTLELCYDVAKLSLGRSALLDA
                     AFERATLYRTRLKRLKEINQPGYSYWYECTSRHFTLALTPLSVADKFKELMAQKPGSW
                     IFTSATLSVNDDLHHFTSRLGIEQAESLLLPSPFDYSRQALLCVPRNLPQTNQPGSAR
                     QLAAMLRPIIEANNGRCFMLCTSHAMMRDLAEQFRATMTLPVLLQGETSKGQLLQQFV
                     SAGNALLVATSSFWEGVDVRGDTLSLVIIDKLPFTSPDDPLLKARMEDCRLRGGDPFD
                     EVQLPDAVITLKQGVGRLIRDADDRGVLVICDNRLVMRPYGATFLASLPPAPRTRDIA
                     RAVRFLAIPSSR"
     gene            2530..3225
                     /gene="tsaB"
                     /locus_tag="ACHKBL_004284"
     CDS             2530..3225
                     /gene="tsaB"
                     /locus_tag="ACHKBL_004284"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HFO5821061.1"
                     /translation="MRILAIDTATEACSVALWNDGTVNAHFELCPREHTQRILPMVQD
                     ILTTSGTSLTDINALAYGRGPGSFTGVRIGIGIAQGLALGADLPMIGVSTLMTMAQGA
                     WRKNGATRVLAAIDARMGEVYWAEYQRDEKGIWHGEETEAVLKPELVHERMQQLSGEW
                     VTVGTGWQAWPDLGKESGLTLCDGEVLLPAAEDMLPIACQMFAEGKTVAVEHAEPVYL
                     RNNVAWKKLPGKE"
     gene            3264..3845
                     /gene="yeaY"
                     /locus_tag="ACHKBL_004285"
     CDS             3264..3845
                     /gene="yeaY"
                     /locus_tag="ACHKBL_004285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein YeaY"
                     /protein_id="HFO5821062.1"
                     /translation="MAVQKNVIKGILAGTFALMLSGCVTVPDAIKGSSPTPQQDLVRV
                     MSAPQLYVGQEARFGGKVVAVQNQQGKTRLEIATVPLDSGARPTLGEPSRGRIYADVN
                     GFLDPVDFRGQLVTVVGPITGAVDGKIGNTPYKFMVMQVTGYKRWHLTQQVIMPPQPI
                     DPWFYGGRGWPYGYGGWGWYNPGPARVQTVVTE"
     gene            4050..5735
                     /gene="fadD"
                     /locus_tag="ACHKBL_004286"
     CDS             4050..5735
                     /gene="fadD"
                     /locus_tag="ACHKBL_004286"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HFO5821063.1"
                     /translation="MKKVWLNRYPADVPTEINPDRYQSLVDMFEQSVARYADQPAFVN
                     MGEVMTFRKLEERSRAFAAYLQQGLGLKKGDRVALMMPNLLQYPVALFGILRAGMIVV
                     NVNPLYTPRELEHQLNDSGASAIVIVSNFAHTLEKVVDKTAVQHVILTRMGDQLSTAK
                     GTVVNFVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTT
                     GVAKGAMLTHRNMLANLEQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELG
                     GQNLLITNPRDIPGLVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGG
                     MPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLV
                     DDDDSEVPPGQPGELCVKGPQVMLGYWQRPDATDEIIKNGWLHTGDIAVMDEEGFLRI
                     VDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKKDPSL
                     TEESLVTFCRRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNKA"
BASE COUNT         1354 a         1428 c         1608 g         1381 t
ORIGIN      
        1 aatatcttaa tctctggtcg atttatgcaa aaccgaccag tattgtcgat aaattatcgt
       61 ctgacttatt ttgcttcgat aaagcttaca ccgcagccac aatcttaatt tcaactttat
      121 acttcgggtt catcaaaccc gcttgtaccg tgcagcgcac cggcgcatga cccgcgacaa
      181 cccaggcatc ccacgcttta ttcatcgccg cgaagtcgtt tttatcggcc aggaaaatgg
      241 tggcatccag aatgctcgat ttattgctgc cctgtttttc cagcacggcg tcaatctgcg
      301 ccagcgtgtt ggcggtttgc tcaaaggcat cggcgtcgag gttttccggt acaccagtgt
      361 agtagagcgt gttgttgtgg attactacat cagaccagcg ggcttcagca tcgatacgaa
      421 cgatagtcat cattttgtcc tcattatact tccattttcg tgctgcaaag actgccacaa
      481 tgtgaggtcg ccgtcaccac ttcaactggc gaaagcgccc tcatcctgac ataatccctg
      541 ttcaaatcaa cagggggtag tgtgacggac gattttgcac cagacggtca gctggcgaaa
      601 gcgataccag gctttaagcc gcgagaacca cagcgacaga tggcggtagc cgtcacccag
      661 gcgatagaaa aaggccagcc gctggtggtg gaagccggaa ccggtacggg caaaacctac
      721 gcttacctgg cccctgcgct gcgggcgaaa aagaaagtca ttatctcgac cggctcaaaa
      781 gcgttgcagg atcagctcta cagccgcgat ttgccaacgg tttcaaaggc gttgaaatat
      841 acgggcaacg tggcgttgct gaaagggcgc tcaaactacc tctgcctcga acgtctcgaa
      901 cagcaggcgc tggcgggggg cgatctgccg gtacaaatct taagcgatgt gatcctgctg
      961 cgctcctggt ctaatcaaac agtcgatggt gatatcagca cctgcgtcag cgtggcggaa
     1021 gattcacagg cgtggccgtt ggtcaccagc accaacgata actgccttgg cagcgactgc
     1081 ccgatgtata aagattgctt tgtggtcaaa gcacgcaaaa aagcgatgga cgccgatgtg
     1141 gtggtggtaa accatcatct ctttctggcg gatatggtgg tgaaagagag tggatttggc
     1201 gaactgatcc cggaagctga cgtcatgatc ttcgacgaag cccaccagct gcctgacatt
     1261 gccagccagt attttggtca gtcactctcc agtcgacaac tactcgacct ggcaaaagac
     1321 atcaccatcg cctatcgcac cgaattaaaa gatactcagc agttacaaaa gtgcgccgac
     1381 cgccttgccc agagcgcgca ggattttcgt ctgcaactcg gtgagcctgg ttatcgtggc
     1441 aacctgcgcg aactgttagc taatccgcaa attcaacggg cgtttttact gctcgatgac
     1501 accctggaac tttgttatga cgtggcgaaa ctgtcgctgg ggcgttccgc cttgctggat
     1561 gcggcatttg agcgcgccac gttgtatcgt acgcggctga aacggctaaa agagatcaat
     1621 cagccgggct acagctactg gtacgaatgc acttcgcgcc attttactct ggcgctcacg
     1681 ccgctcagcg tggcggataa attcaaagag ttaatggcgc aaaaacccgg tagctggatc
     1741 tttacctcag caacgctgtc ggtgaacgac gatctgcatc attttacctc gcggcttggc
     1801 attgaacagg cagagtcgtt gctgttaccc agcccgtttg attacagccg ccaggcgtta
     1861 ctctgtgtgc cgcgcaacct gccgcaaacc aatcaaccgg gctccgcacg gcaactggcg
     1921 gcaatgttgc gaccgatcat cgaagctaac aacggtcgtt gttttatgct ttgtacctcg
     1981 cacgccatga tgcgcgatct ggctgagcag ttccgcgcta ccatgacgct tcccgttttg
     2041 ttgcaggggg aaaccagcaa agggcaactg ttgcagcaat ttgtcagcgc cggtaacgcg
     2101 cttcttgtgg caaccagcag cttctgggaa ggggtggatg tgcgtggcga tacattgtca
     2161 ttggtgatta tcgacaagtt gccgtttacc tcaccggatg atccactatt aaaagcgcgc
     2221 atggaagatt gccgtttgcg tggtggtgac ccgttcgatg aagtacaact gccggatgcg
     2281 gtgattactc tcaagcaggg agtagggcga ctgattcgcg acgccgacga tcgcggcgtg
     2341 ctggtgattt gcgataatcg gctggtgatg cgcccttacg gcgcgacgtt tctcgccagt
     2401 ctgccgccag cgccgcgcac ccgtgacatt gcccgtgcgg ttcgtttcct tgcgatacca
     2461 tcctccaggt aatttgttac ggagtatggt atgatgcgcg cctttttatt gatctaagca
     2521 cgaagaccca tgcgaattct ggctatcgat accgcgacag aggcctgctc tgtcgccctg
     2581 tggaacgacg gtactgtcaa cgctcatttt gagctttgcc ctcgtgaaca tactcaacga
     2641 atcttaccga tggtgcagga tatcctgacc accagcggaa cttccctgac tgatattaac
     2701 gctctggctt acgggcgcgg ccccggtagc tttaccggcg tgcgcattgg tattggtatt
     2761 gcgcaggggc tggcgcttgg cgcggatctg ccgatgattg gcgtttccac gctcatgaca
     2821 atggcgcaag gggcgtggcg caaaaacggc gcaacccgcg tgctggctgc tattgatgcg
     2881 cggatgggcg aagtttactg ggccgaatat cagcgtgatg aaaagggtat ctggcacggt
     2941 gaagaaaccg aagccgtact caaacccgaa ctcgttcatg aacggatgca acagctttcc
     3001 ggggaatggg tgacggtagg tacaggctgg caagcctggc cggatctcgg taaagagagc
     3061 ggtttgaccc tgtgcgatgg cgaagtgtta ctgcccgctg cagaagatat gcttccgatt
     3121 gcgtgtcaga tgtttgccga gggtaaaacg gtggcggtgg aacatgccga accggtttat
     3181 ttacgtaaca acgtcgcatg gaagaaactt ccgggcaaag aatgaatctt aagagtatgc
     3241 cctgagaaaa aaggagtcgc atcatggcgg ttcaaaagaa tgttatcaaa ggcatactgg
     3301 caggtacgtt tgcgctaatg ctcagcggtt gtgttactgt gccggacgcc attaaaggca
     3361 gcagtcccac gccgcaacaa gatttagttc gggtgatgag tgcgccgcag ctgtacgttg
     3421 gtcaggaggc acgctttggt ggcaaagtgg ttgcggtaca aaaccagcaa gggaaaaccc
     3481 gcctggaaat tgccaccgta ccactggaca gcggagccag accgacgctg ggagaacctt
     3541 ctcgcggtcg catttatgct gatgtgaacg gttttctgga cccggtagat ttccgtggac
     3601 aactggttac ggtagttggg ccaatcaccg gtgcggttga cggcaaaatc ggcaacacgc
     3661 cctataaatt tatggtgatg caggtaacgg gttacaaacg ttggcattta acccagcagg
     3721 tgattatgcc gcctcagccg attgatccat ggttttatgg cggtcgtggc tggccctatg
     3781 gctacggtgg atggggctgg tataatcccg gccccgcgag agtacaaact gttgtaactg
     3841 aataattgct tgtttttaaa gaaaaagaaa cagcggctgg tccgctgttt ctgcattctt
     3901 acggtaaaga taaaaataaa tagtgacgcg cttcgcaacc ttttcgttgg gtaattatca
     3961 agctggtatg atgagttaat attatgttaa cagcatgtat atcatttggg gttgcgatga
     4021 cgacgaacac gcattttaga ggtgaagaat tgaagaaggt ttggcttaac cgttatcccg
     4081 cggacgtccc gacggagatc aaccctgacc gttatcaatc tctggtagat atgtttgagc
     4141 agtcggtcgc gcgctacgcc gatcaacccg cgtttgtgaa tatgggggag gtgatgacct
     4201 tccgcaaact ggaagaacgc agtcgcgcgt ttgccgctta tttgcaacaa gggttgggac
     4261 tgaagaaagg cgatcgcgtt gcgttgatga tgcctaattt attgcaatat ccggtggcgc
     4321 tgtttggcat tttgcgtgcc gggatgattg tcgtaaacgt taacccgttg tataccccgc
     4381 gtgaacttga gcatcagctt aacgatagcg gcgcatcggc gattgttatc gtgtctaact
     4441 ttgcccacac tctggaaaaa gtggttgata aaaccgccgt tcagcacgta attctgaccc
     4501 gtatgggcga tcagctatct acggcaaaag gcacggtagt caatttcgtt gttaaataca
     4561 tcaagcgtct ggtgccgaaa taccatctgc cagatgccat ttcatttcgt agcgccctgc
     4621 acaacggcta ccggatgcag tacgtcaaac ccgaactggt gccggaagat ttagctttcc
     4681 tgcaatacac cggcggcacc actggtgtgg cgaaaggcgc gatgctgact caccgcaata
     4741 tgctggcgaa cctggaacag gttaacgcga cctatggtcc gctgttgcat ccgggcaaag
     4801 agctggtggt gacggcgctg ccgctgtatc acatttttgc actgaccatt aactgcctgc
     4861 tgtttatcga actggggggg cagaacctgc ttatcactaa cccgcgcgat attccagggt
     4921 tggtaaaaga gttagcgaaa tatccgttta ccgctatcac tggcgttaac accttgttca
     4981 atgcgttgct gaacaataaa gagttccagc agctggattt ctccagtctg catctttccg
     5041 caggcggagg gatgcctgtt cagcaagtgg tggcagaacg ttgggttaaa ctgactggac
     5101 agtatctgct ggaaggttat ggcctgaccg agtgtgcgcc gctggtgagc gttaacccgt
     5161 atgatattga ttatcatagt ggtagcatcg gtttgccggt gccgtcgacg gaagccaaac
     5221 tggtggatga tgatgatagt gaagtaccac ctggtcaacc gggtgagctt tgtgtcaaag
     5281 gaccgcaggt gatgctgggt tactggcagc gtccggatgc taccgatgaa atcatcaaaa
     5341 atggctggtt acacaccggc gacatcgcgg tgatggatga agaaggattc ctgcgcattg
     5401 tcgatcgtaa aaaagacatg attctggttt ccggttttaa cgtctatccc aacgagattg
     5461 aagatgtcgt catgcagcat cctggcgtac aggaagtcgc ggctgttggc gtaccttccg
     5521 gctccagtgg tgaagcggtg aaaatcttcg ttgtgaaaaa agatccatcg cttaccgaag
     5581 agtcactggt gactttttgc cgccgtcagc tcacgggcta caaagtaccg aagctggtgg
     5641 agtttcgtga tgagttaccg aaatctaacg tcggaaaaat tttgcgacga gaattacgtg
     5701 acgaagcgcg cggcaaagtg gacaataaag cctgagcgtt aagtcagtcg tcagacgccg
     5761 gttaatccgg c
//
LOCUS       DAZFLD010000245         5647 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.246, whole genome shotgun sequence.
ACCESSION   DAZFLD010000245 DAZFLD010000000
VERSION     DAZFLD010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5647
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.246"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            84..1592
                     /gene="casA"
                     /locus_tag="ACHKBL_004287"
     CDS             84..1592
                     /gene="casA"
                     /locus_tag="ACHKBL_004287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse1/CasA"
                     /protein_id="HFO5821064.1"
                     /translation="MNLLIDNWIPVRPRNGGKVQIINLQSLYCSRDQWRLSLPRDDME
                     LATLALLVCIGQIIAPAKDDVEFRHRIMNPLTEDEFQQLIAPWIDMFYLNHAEHPFMQ
                     TKGVKANDVTPMEKLLAGVSGATNCAFVNQPGQGEALCGGCTAIALFNQANQAPGFGG
                     GFKSGLRGGTPITTFVCGIDLRSTVLLNVLTIPRLQKQFPNELHTENQPTWVKPVKPN
                     ESVPASSIGFVRGLFWQPAHIELCDPIGIGKCSCCGQESNLRYTGFLKEKFTFTVNGL
                     WPHPHSPCLVTVKKGEVEEKFLAFTTSAPSWTQISRVVVDKIIQNENGNRVAAVVNQF
                     RNIAPQSPLELIMGGYRNNQASILERRHDVLMFNQGWQQYGNVINEIVTVGLGYKTAL
                     RKALYTFAEGFKNKDFKGAGVSVHETAERHFYRQSELLIPDVLANINFSQADEVIADL
                     RDKLHQLCEMLFNQSVAPYAHHPKLISTLALARATLYKHLRELKPQGGPSNG"
     gene            1585..2067
                     /gene="casB"
                     /locus_tag="ACHKBL_004288"
                     /gene_synonym="cse2"
     CDS             1585..2067
                     /gene="casB"
                     /locus_tag="ACHKBL_004288"
                     /gene_synonym="cse2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse2/CasB"
                     /protein_id="HFO5821065.1"
                     /translation="MADEIDAMALYRAWQQLDNGACAQIRRVSEPDELRDIPAFYRLV
                     QSFGWENPRHQQALLRMVFCLSAGKNVIRHQDKKTEQTTGISLGRALANSGRINERRI
                     FQLIRADRTADMVQLRRLLTHAEPVLDWPLMARMLTWWGKRERQQLLEDFVLTTNKNA
                     "
     gene            2080..3171
                     /gene="cas7e"
                     /locus_tag="ACHKBL_004289"
     CDS             2080..3171
                     /gene="cas7e"
                     /locus_tag="ACHKBL_004289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas7/Cse4/CasC"
                     /protein_id="HFO5821066.1"
                     /translation="MSNFINIHVLISHSPSCLNRDDMNMQKDAIFGGKRRVRISSQSL
                     KHAMRKSDYYAQNIGESSLRTIHLAQLRDVLRQKLGERFDQKIIDKTLALLSGKLVDE
                     AEKISADAVTPWVVGEIAWFCEQVAKAEADNLDDKKLLKVLKEDIAAIRVNLQQGVDI
                     ALSGRMATSGMMSELGKVDGAMSIAHAITTHQVDSDIDWFTAVDDLQEQGSAHLGTQE
                     FSSGVFYRYANINLAQLQENLGGASREQALEIATHVVHMLATEVPGAKQRTYAAFNPA
                     DMVMVNFSDMPLSMANAFEKAVKAKDGFLQPSIQAFNQYWDRVANGYGLNGAAAQFSL
                     SDVDPITAQVKQMPTLEQLKSWVRNNGEA"
     gene            3174..3848
                     /gene="cas5e"
                     /locus_tag="ACHKBL_004290"
     CDS             3174..3848
                     /gene="cas5e"
                     /locus_tag="ACHKBL_004290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cas5/CasD"
                     /protein_id="HFO5821067.1"
                     /translation="MRSYLILRLAGPMQAWGQPTFEGTRPTGRFPTRSGLLGLLGACL
                     GIQRDDTSSLQALSESVQFAVRCDELILDDRRVSVTGLRDYHTVLGAREDYRGLKSHE
                     TIQTWREYLCDASFTVAIWLTPQATMVMSELEKAVLKPRYTPYLGRRSCPLTQPLFLG
                     TCQASDPQKALLNYEPVGGDIYSEESVDGHHLKFTVRDEPMITLPRQFASREWYVIKG
                     GMDVSQ"
     gene            3835..4434
                     /gene="cas6e"
                     /locus_tag="ACHKBL_004291"
     CDS             3835..4434
                     /gene="cas6e"
                     /locus_tag="ACHKBL_004291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas6/Cse3/CasE"
                     /protein_id="HFO5821068.1"
                     /translation="MYLSKVIIARAWSRDLYQLHQGLWHLFPNRPDAARDFLFHVEKR
                     NTPEGCHVLLQSAQMPVSTAVATVIKTKQVEFQLQVGVPLYFRLRANPIKTILDNQKR
                     LDSKGNIKRCRVPLIKEAEQIAWLQRKLGNAARVEDVHPISERPQYFSGDGKSGKIQT
                     VCFEGVITINDAPALIDLVQQGIGPAKSMGCGLLSLAPL"
     gene            4450..5367
                     /gene="cas1e"
                     /locus_tag="ACHKBL_004292"
     CDS             4450..5367
                     /gene="cas1e"
                     /locus_tag="ACHKBL_004292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004520 - DNA endonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endonuclease Cas1e"
                     /protein_id="HFO5821069.1"
                     /translation="MTWLPLNPIPLKDRVSMIFLQYGQIDVIDGAFVLIDKTGIRTHI
                     PVGSVACIMLEPGTRVSHAAVRLAAQVGTLLVWVGEAGVRVYASGQPGGARSDKLLYQ
                     AKLALDEDLRLKVVRKMFELRFGEPAPARRSVEQLRGIEGSRVRATYVLLAKQYGVKW
                     NGRRYDPKDWEKGDTVNQCISAATSCLYGVTEAAILAAGYAPAIGFVHTGKPLSFVYD
                     IADIIKFDTVVPKAFEIARRIPGEPDREVRLACRDIFRSSKTLVKLIPLIEDVLAAGE
                     IQPPAPPEDAQPVAIPLPVSLGDAGHRSS"
     gene            5369..>5647
                     /gene="cas2e"
                     /locus_tag="ACHKBL_004293"
     CDS             5369..>5647
                     /gene="cas2e"
                     /locus_tag="ACHKBL_004293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endoribonuclease
                     Cas2e"
                     /protein_id="HFO5821070.1"
                     /translation="MSMLVVVTENVPSRLRGRLAIWLLEVRAGVYVGDVSARVREMIW
                     EQISGLAEEGNVVMAWATNTESGFEFQTFGVNRRTPVDLDGLRLVSFLP"
BASE COUNT         1521 a         1154 c         1431 g         1541 t
ORIGIN      
        1 tttttctttg tttttaccga taacggaagt gtgccgacgc atagaaatgc aggagaaatg
       61 tcggagcata tgaaggagaa caaatgaatt tgcttattga taactggatc cctgtacgcc
      121 cgcgaaacgg ggggaaagtc caaatcataa atctgcaatc gctatactgc agtagagatc
      181 agtggcgatt aagtttgccc cgtgacgata tggaactggc cactttagca ctgctggttt
      241 gcattgggca aattatcgcc ccggcaaaag atgacgttga atttcgacat cgcataatga
      301 atccgctcac tgaagatgag tttcaacaac tcatcgcgcc gtggatagat atgttctacc
      361 ttaatcacgc agaacatccc tttatgcaga ccaaaggtgt caaagcaaat gatgtgactc
      421 caatggaaaa actgttggct ggggtaagcg gcgcgacgaa ttgtgcattt gtcaatcaac
      481 cggggcaggg tgaagcatta tgtggtggat gcactgcgat tgcgttattc aaccaggcga
      541 atcaggcacc tggttttggt ggtggcttta aaagcggttt acgtggagga acacctataa
      601 ccaccttcgt atgtgggatc gatcttcgct cgacggtatt actcaatgtc ctcacaatac
      661 ctcgtcttca aaaacaattt cccaatgaat tacatacgga aaaccaacct acctgggtta
      721 agcctgtcaa gccgaatgaa tcggtgcctg cttcttcaat tgggtttgtc cgtggtttat
      781 tctggcaacc agcgcatatt gaactatgcg atcccattgg gattggtaaa tgttcttgct
      841 gtggtcagga aagcaatttg cgttataccg gttttcttaa ggaaaaattt acctttacag
      901 ttaatgggct atggccccat ccgcattccc cttgtctggt aacagtcaag aaaggggagg
      961 ttgaggaaaa atttcttgct ttcaccacct ccgcaccatc atggacacaa atcagccgag
     1021 ttgtggtaga taagattatt caaaatgaaa atggaaatcg cgtggcggcg gttgtgaatc
     1081 aattcagaaa tattgcgccg caaagtcctc ttgaattgat tatgggggga tatcgtaata
     1141 atcaagcatc tattcttgaa cggcgtcatg atgtgttgat gtttaatcag gggtggcaac
     1201 aatacggcaa tgtgataaac gaaatagtga ctgttggttt gggatataaa acagccttac
     1261 gcaaggcgtt atataccttt gcagaagggt ttaaaaataa agacttcaaa ggggccggag
     1321 tttctgttca tgagactgca gaaaggcatt tctatcgaca gagtgaatta ttaattcccg
     1381 atgtattggc gaatattaat ttttcccaag ctgatgaggt aatagctgat ttacgagaca
     1441 aacttcatca attgtgtgaa atgctattta atcaatctgt agctccctat gcacatcatc
     1501 ctaaattaat aagcacatta gcgcttgccc gcgccacgct atacaaacat ttacgggagt
     1561 taaaacctca aggagggcca tcaaatggct gatgaaattg atgcaatggc tttatatcga
     1621 gcctggcaac aactggataa tggagcatgt gcgcaaatta gacgtgtttc agaacctgat
     1681 gaattacgcg atatccctgc gttttatagg ctggtgcaat cttttggttg ggaaaaccca
     1741 cgtcaccagc aggctctttt gcgcatggtg ttttgcctga gcgcaggaaa gaatgtcatc
     1801 cgacatcagg acaaaaaaac ggagcaaaca acaggtatct cgttgggaag agctttagcc
     1861 aatagtggaa gaattaacga gcgccgtatc tttcaattaa ttcgggctga cagaacagcc
     1921 gatatggtcc agttacgtcg attacttact cacgccgaac ccgtacttga ctggccatta
     1981 atggccagga tgttgacctg gtggggaaag cgcgaacgcc agcaacttct ggaagatttt
     2041 gtattgacca caaacaaaaa tgcgtaagga aacctttcta tgtctaactt tatcaatatt
     2101 catgttctga tctctcacag cccttcatgt ctgaaccgcg acgatatgaa catgcagaaa
     2161 gacgctattt tcggcgggaa aagacgagta agaatttcaa gtcaaagcct taaacatgcg
     2221 atgcgtaaaa gtgattatta cgcacaaaat attggtgaat ccagtctcag aacaattcat
     2281 cttgcacaat tacgtgatgt tcttcggcaa aaacttggtg aacgttttga ccaaaaaatc
     2341 atcgataaga cattagcgct gctctccggt aaattagttg atgaagccga aaagatttct
     2401 gccgatgcgg ttactccctg ggttgtggga gaaatagcct ggttctgtga gcaggttgca
     2461 aaagcagagg ctgataatct ggatgataaa aagctgctca aagttcttaa ggaagatatt
     2521 gccgccatac gtgtgaattt acagcagggt gttgatattg cgcttagtgg aagaatggca
     2581 accagcggca tgatgagtga gttgggaaaa gttgatggtg caatgtccat tgcgcatgcg
     2641 atcactactc atcaggttga ttctgatatt gactggttca ccgctgtaga tgatttacag
     2701 gaacaaggtt ctgcacatct gggaactcag gaattttcat cgggtgtttt ttatcgttat
     2761 gccaacatta acctcgctca acttcaggaa aatttaggtg gtgcctccag ggagcaggct
     2821 ctggaaattg caacccatgt tgttcatatg ctggcaacag aggtccctgg agcaaaacag
     2881 cgtacttatg ccgcttttaa ccctgcggat atggtaatgg ttaatttctc cgatatgcca
     2941 ctttctatgg caaatgcttt tgaaaaagcg gttaaagcga aagatggctt tttgcaaccg
     3001 tctatacagg cgtttaatca atattgggat cgcgttgcca atggatatgg tctgaacgga
     3061 gctgctgcgc aattcagctt atctgatgta gacccaatta ctgctcaagt taaacaaatg
     3121 cctactttag aacagttaaa atcctgggtt cgtaataatg gcgaggcgtg aacatgagat
     3181 cttatttgat cttgcggctt gctgggccaa tgcaagcctg ggggcagccg acctttgaag
     3241 gaacgcgacc taccggaaga tttccgaccc gaagcgggtt attagggcta ctcggggctt
     3301 gtcttgggat ccaacgtgat gatacttctt cattacaggc gttatcagag agtgtgcaat
     3361 ttgcagtgcg ctgcgatgaa ctcattcttg acgatcgtcg tgtgtctgta acggggttgc
     3421 gtgattacca tacagtcctt ggagcgcgag aagattaccg tggtttgaaa agtcatgaaa
     3481 cgattcaaac atggcgcgaa tatttatgtg atgcctcctt taccgtcgct atctggttaa
     3541 caccacaggc aacgatggtt atgtcagaac ttgaaaaagc agtattaaag cctcggtata
     3601 caccgtatct ggggcggagg agttgtccgc taacacaacc gctttttctc gggacatgtc
     3661 aggcgtcgga tcctcagaag gcgctattaa attatgagcc cgttggcggc gatatttata
     3721 gtgaggaatc agttgacggg catcatttaa aatttacggt gcgcgacgaa ccgatgatca
     3781 ccttacctcg acaatttgct tcccgagaat ggtatgtgat taaaggaggt atggatgtat
     3841 ctcagtaaag tcatcattgc cagggcctgg agcagggatc tttaccaact tcaccaggga
     3901 ttatggcatt tatttccaaa cagaccggat gctgctcgtg attttctttt tcatgttgag
     3961 aagcgaaaca caccagaagg ctgtcatgtt ttattgcagt cagcgcaaat gcctgtttct
     4021 actgccgttg cgacagtcat taaaactaaa caggttgaat ttcaacttca ggttggtgtt
     4081 ccactctatt ttcggcttcg ggcaaatccg atcaaaacta ttctcgacaa tcaaaagcgc
     4141 ctggacagta aagggaatat taaacgctgt cgggttccgt taataaaaga agcagaacaa
     4201 atcgcgtggt tgcaacgtaa attgggcaat gcggcgcgcg ttgaagatgt gcatcccata
     4261 tcggaacggc cacagtattt ttctggtgat ggtaaaagtg gaaagatcca aacggtttgc
     4321 tttgaagggg tgatcaccat caacgacgcg ccagcgttaa tagatcttgt acagcaaggt
     4381 attgggccag ctaaatcgat gggatgtggc ttgctatctt tggctccact gtgattgagg
     4441 tgtaataaaa tgacctggct tccccttaat cccattccac tcaaagatcg cgtctccatg
     4501 atctttctgc aatatgggca gatcgatgta atagatggcg cgtttgtact tatcgacaag
     4561 acagggatcc gcactcatat tcctgttggc tcggttgcct gcatcatgtt ggaacctggt
     4621 acacgggttt cgcatgcagc tgtacgcctg gctgcgcaag ttggaacatt attggtatgg
     4681 gtgggggaag cgggcgttcg tgtttatgct tctggtcagc ctggtggtgc gcgttcagat
     4741 aagctgctct atcaggcaaa acttgctctg gatgaagatt tgcgtctgaa ggtcgtacgt
     4801 aaaatgtttg aacttcggtt tggagaacct gcgcctgccc ggcgctccgt agagcaactc
     4861 agaggtatag aaggaagtcg tgtgcgagca acctatgtac ttctggcgaa gcaatacggg
     4921 gtgaaatgga atggacgtcg ttacgatccg aaagactggg aaaagggcga tacggtcaac
     4981 caatgcatta gcgctgcaac ttcttgttta tatggcgtaa ctgaagcggc gatacttgca
     5041 gctggttatg caccagctat tgggtttgtg catacaggaa agcctctttc ctttgtttac
     5101 gatatcgcgg acatcattaa atttgacact gttgtaccga aagcttttga gatagcgcgt
     5161 cgtattcctg gtgagccgga ccgggaagtc cgtttggcgt gcagggatat ttttcgcagt
     5221 agtaaaacat tagtcaaatt gattccgctt atagaggacg tgcttgctgc tggggaaata
     5281 cagccgccgg ccccacctga agatgcgcag cctgttgcta ttccacttcc cgtttctctg
     5341 ggagatgccg gacatcggag tagctgagat gagtatgttg gtcgtggtca ctgaaaatgt
     5401 accttcgcgc ttacgaggca gattagccat ctggttgttg gaggtacgtg caggggtgta
     5461 tgtaggtgat gtatccgcaa gagtccgtga aatgatctgg gaacaaatat ctggactggc
     5521 ggaagaaggc aatgtggtga tggcatgggc tacgaatacg gaatcgggat ttgagttcca
     5581 gacatttggg gtaaacaggc gtactccggt agatttggat ggtttaaggt tggtctcttt
     5641 tttacct
//
LOCUS       DAZFLD010000246         5641 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.247, whole genome shotgun sequence.
ACCESSION   DAZFLD010000246 DAZFLD010000000
VERSION     DAZFLD010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5641
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.247"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            82..951
                     /locus_tag="ACHKBL_004294"
     CDS             82..951
                     /locus_tag="ACHKBL_004294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeJ family protein"
                     /protein_id="HFO5821071.1"
                     /translation="MAISIKGVNTGVIRKSNNFIALALKIKEPRNKESLFFLSVMELR
                     DLLIALESRLHQKHKLDAAARLQYEQARDKVIKKMVENIPEILVDELKNADINRRVNT
                     LELTDNQGENLTFVLTLHDGSKCELVINELQIEMLARAIIHAINNAEMRELALRITSL
                     LDFLPLYDVDCQDNGNLEYDTYSQPEWKHNLFNHYLAVLYRFKDESGKEQFSGAVVKT
                     REATPGKEVEAITRRMLDFSPRLKKLAGVPCQVYVRTVAANNAQPLTQDQCLRALHHL
                     RVQSTSKTAPQAK"
     gene            complement(1001..1354)
                     /locus_tag="ACHKBL_004295"
     CDS             complement(1001..1354)
                     /locus_tag="ACHKBL_004295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HFO5821072.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(1492..3138)
                     /gene="groL"
                     /locus_tag="ACHKBL_004296"
     CDS             complement(1492..3138)
                     /gene="groL"
                     /locus_tag="ACHKBL_004296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HFO5821073.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            complement(3182..3475)
                     /locus_tag="ACHKBL_004297"
     CDS             complement(3182..3475)
                     /locus_tag="ACHKBL_004297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HFO5821074.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            3751..5007
                     /gene="yjeH"
                     /locus_tag="ACHKBL_004298"
     CDS             3751..5007
                     /gene="yjeH"
                     /locus_tag="ACHKBL_004298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HFO5821075.1"
                     /translation="MSGLKQELGLAQGIGLLSTSLLGTGVFAVPALAALVAGNNSLWA
                     WPVLIILVFPIAIVFAILGRHYPSAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSWQLLLAELGTLALVWYIGTRGASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPANIPFPAPGNIELTGLFAALSVMFWCFVGLEAFAHLASEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEKMAAAASLPKIVVQLFGVGALWIACVIGY
                     LACFASLNIYIQSFARLVWSQAQHNPDHYLSRLSSRHIPNNALNAVLGCCVVSTLVIH
                     ALEINLDALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLTMVGWKS
                     LYALIMLAGLWLFLPKRKTPENGITT"
     gene            complement(5023..5499)
                     /locus_tag="ACHKBL_004299"
     CDS             complement(5023..5499)
                     /locus_tag="ACHKBL_004299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxsA family protein"
                     /protein_id="HFO5821076.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDDRDRLDHKDDRQD"
BASE COUNT         1336 a         1464 c         1355 g         1486 t
ORIGIN      
        1 aactattact accaagccca tttatctggc ttataaatta aaattaatta tttaattgag
       61 aaatggttag ggagaaccta catggccata agcatcaagg gtgttaatac cggtgttatt
      121 cgcaagagca ataatttcat tgcactggcg ctgaaaatta aagagcctcg caataaagaa
      181 tcgttgttct ttctgtctgt catggaactg cgcgaccttc tgattgcgct ggaaagccgt
      241 ctgcatcaaa agcataaact ggatgctgct gcacgccttc agtatgagca agctcgcgat
      301 aaagtcatta agaaaatggt agaaaatatc ccagaaattc tggttgatga gcttaaaaat
      361 gccgatatta atcgtcgggt taacacgctg gaacttaccg ataatcaggg cgaaaatctt
      421 accttcgtat taacactaca tgacggtagc aaatgtgagt tagtcatcaa tgaattacag
      481 attgaaatgc tggcgcgagc aatcattcat gcaattaata atgcggaaat gcgtgaactg
      541 gcattacgta ttacttcact gttagatttc ctgccgttat acgacgttga ctgccaggac
      601 aatggcaatc tggaatatga cacctattcc caaccggaat ggaaacataa cttgttcaac
      661 cattatctgg cggtgcttta ccgctttaaa gatgaaagtg gcaaagaaca atttagcggc
      721 gcagtggtga aaacgcgtga agcaacgccg ggtaaagaag tggaggcgat cactcgccga
      781 atgcttgatt tcagcccccg actgaaaaaa ctcgcgggtg tgccatgtca ggtctatgtt
      841 cgcaccgtag cggcaaataa cgcccaaccg ctaacccagg atcaatgctt acgcgcgctg
      901 catcacttac gtgttcaatc caccagcaaa actgcaccac aggcgaaata atctcatctg
      961 ttttactacg aggctggaag tatccagcct ctggatcaca tcagttcggg cacttataaa
     1021 cctgaccgat aatctggcta tccgtcggga caaaactgga caacattccc tgcgacgggc
     1081 tgctgatgcc ataaatcacg ttaccgccca ttgcagccgc ctggttgcgc agatcgtttg
     1141 ctgcgccgcg catagaaccg ccctcttctc cgtgttgccc ggaaagccag ttgctttgct
     1201 tacctgtcgc agtaccaatc agctggcact ctgcgcctgg ctgctcgtcc acaatgcgta
     1261 cactctgacc ggcagcactc aattcgttgc tggagctaca acccgccatc agtagcgcgg
     1321 caccgacaat ccctgctaag tattttacgt gcatgttatt ccccataatc aatgagctgg
     1381 acgccactcg cgtcgtccgt gtctgaatct tatactagaa agatgaccaa aagaaaaacc
     1441 cccagacatt tctgcccggg ggtttgttta tttctgcgag gtgcagggca attacatcat
     1501 gccgcccatg ccacccatgc cgcccatacc gccagcagcg cctaagtcag ctgcatcgtt
     1561 tttcggcagg tcggtaacca tgcattcggt ggtgatcatc aggccagcca cagaagctgc
     1621 gtactgcaga gcagaacgag ttactttggt tggatccagg atacccatgt cgatcatgtt
     1681 gccgtattct tcggttgctg cgttgtaacc gtagttgccg tcgccgcctt taacggtgtt
     1741 agcaacaaca gacggttctt cgccgcagtt caatacgatc tgacgcagcg gagcttccat
     1801 tgcacgcagt gcaactttga tacccacgtt ctggtcttcg ttctgaccac gcaggtcagc
     1861 cagtttagac gctacgcgga tcagcgcaac accaccacca gcaaccacgc cttcttctac
     1921 cgcagcacgg gtcgcgtgca gggcatcttc aacgcgtgct tttttctctt tcatttcaac
     1981 ttcggtagca gcacccactt tgataactgc aacgccgcct gccagtttcg ctacgcgctc
     2041 ctgcagtttt tcacggtcgt agtcagaagt tgcttcttca atctgctgac ggatctgagc
     2101 aacacggccc tggattgcag cttcttcacc cacgccatca atgatggtgg tggtgtcttt
     2161 gttgatcaca acacgtttag cctgacccag gtcttccagg gttgcttttt ccagctccat
     2221 accgatctct tcagagatta cggtaccgcc agtcagggtt gcgatatcct gcagcatagc
     2281 tttacgacga tcgccgaagc ccggagcttt aaccgcagca actttcacga tgccacgcat
     2341 ggtgttaaca accagagttg ccagcgcttc gccttctaca tcttcagcga tgatcagcag
     2401 cggtttgcct gctttggcaa cagcttccag aaccggcagc atttcgcgga tgttggagat
     2461 tttcttgtca gccagcagga tgaacgggct ttccagttct actgcgccag tttccggctt
     2521 gttgataaag taaggagaca ggtagccacg gtcgaactgc ataccttcaa ccacgtccag
     2581 ttcgtcctgc agaccggtac cgtcttcaac ggtgataacg ccttctttac cgactttgtc
     2641 catcgcttca gcgatcagtt tacctacggt ttcgtcggag ttagcggaga tggtaccaac
     2701 ctgagcaatc gctttagagt cagagcatgg tacggacagc gctttcagtt cttcaactgc
     2761 agcggtaacc gctttgtcga taccacgttt caggtccatc gggttcatgc ccgcagcaac
     2821 agctttcaga ccttcagtga tgatagcctg agccagtacg gttgcagtgg tggtaccgtc
     2881 gcctgcagcg tcgttcgctt tggaggcaac ttctttcacc atctgcgcac ccatgttttc
     2941 gaacttgtct tccagttcga tttcacgagc aacggaaaca ccatctttgg tgatggtcgg
     3001 tgcaccgaaa gatttatcca gaactacgtt acggcctttc ggaccgaggg taactttcac
     3061 tgcatctgcc agtacgttta cgccgcgcag cattttcaca cgagcgtcgt taccgaattt
     3121 tacgtcttta gctgccatta tctttattcc ttaaattcgt atgttcagtg tcgtgcgcgg
     3181 attacgcttc aacaattgcc agaatgtcgc tttcggacat gatcaacact tcttcattgt
     3241 cgatcttctc ggatttcaca ccgtagccat cgttgaaaat aacgatgtcg ccaactttca
     3301 cgtccagcgg cttcacttcg ccattttcaa ggatacggcc attgccgaca gccagcactt
     3361 cgccgcgggt ggatttagcc gctgcagagc cggtcagaac gatgccgcca gcagatttag
     3421 tttcaacttc tttacgcttg acgatcacgc gatcatgcaa tggacgaata ttcattgata
     3481 actctccttt gagaaagtcc gtatctgtta tgggtgacgc cggagcttac gtggtttccc
     3541 ggctggtgac cagagaaatg gggatgaggc ttcgcccctt caagggggaa aaagaaaaaa
     3601 aattctgatc agcacaaaat cgggtgaaaa ccctgattca cctcacattt catcgcaatt
     3661 tcttcatcgc cgtcataagc gaatctgatt gtgctaccat cgaaaatcta cgcagttgcc
     3721 caaaatttgg gcgcaatcgg acatcaaccc atgagtggac tcaaacaaga actggggctg
     3781 gcccagggca ttggcctgct atcgacgtca ttattaggca ctggcgtgtt tgccgttcct
     3841 gcgttagctg cactggtagc gggcaataac agcctgtggg cgtggcccgt tttgattatc
     3901 ttagtgttcc cgattgcgat tgtgtttgcg attctgggtc gccactatcc cagcgcaggc
     3961 ggcgtcgcgc acttcgtcgg tatggcgttt ggttcgcggc ttgagcgagt caccggctgg
     4021 ctgtttttat cggtcattcc cgtgggtttg cctgccgcac tacaaattgc cgccgggttc
     4081 ggccaggcaa tgtttggctg gcatagctgg caactgttgt tggcagaact cggtacgctg
     4141 gcgttggtgt ggtatatcgg tactcgcggt gccagttcca gtgcaaatct acaaaccgtt
     4201 attgccggac ttatcgtcgc gctgattgtc gctatctggt gggcgggcga tatcaaacct
     4261 gcgaatatcc ccttcccggc acctggtaat atcgaactta ccgggttatt tgctgcgtta
     4321 tcagtgatgt tctggtgttt tgtcggtctg gaggcatttg cccatctcgc ctcggaattt
     4381 aaaaatccag agcgtgattt tcctcgtgct ttgatgattg gtctgctgct ggcaggatta
     4441 gtctactggg gatgtacggt agtcgtctta cacttcgacg cctatggtga aaaaatggcg
     4501 gcggcagcat cgcttccaaa aattgtagtg cagttgttcg gtgtaggagc gttatggatt
     4561 gcctgcgtga ttggctatct ggcctgcttt gccagtctca acatttatat acagagcttc
     4621 gcccgcctgg tctggtcgca ggcgcaacat aatcctgacc actacctgtc acgcctctct
     4681 tctcgccata tcccgaataa tgccctcaat gcggtgctcg gctgctgcgt ggtgagcact
     4741 ttggtgattc atgctttaga gatcaatctg gacgctctta ttatttatgc caatggcatc
     4801 tttattatga tttatctgtt atgcatgctg gcaggctgta aattattgca aggacgttat
     4861 cgactactgg cggtggttgg cgggctgtta tgcgttctgt tactgacaat ggtcggctgg
     4921 aaaagtctct atgcgctgat catgctggcg gggttatggc tgtttctgcc aaaacgaaaa
     4981 acgccggaaa atggcataac cacataatcc ggcgtttcga cattaatcct ggcgatcgtc
     5041 tttatgatca aggcggtcgc ggtcatcatc ctttcgctgg tactcaccat caaaagtatt
     5101 accgccaccg gtcccggcgc taaaaccgcc gcctggcatg cgagaaaagc gcaaatgcgg
     5161 catcaacttc actgtcagat gcttttgcac cggcggcaac aaaagcagaa gaccgaggaa
     5221 gtcggtgaaa aagcccggta ataaaagcag caaaccggca atgatcagcg aaacactttt
     5281 aatcatctcc gccgctgggt tttcacccgc cgccattttt tgctgcatca gcacgaaatt
     5341 cttaaagccc tggttacgta ccagtgacat accgataact gacgtgaata taaccagcac
     5401 gagagtcagc aataccccca atacatgggc aacctgaata aagattgaaa tctcaatata
     5461 gacataaagg aaaatggcaa taaaaggtaa ccagcgcaaa ggtttctcct gtaatagcag
     5521 ccggttaaac ccggctacct gaatgggttg cgaatcgcgt tcagcttata ttgtggtcat
     5581 tagcaaaatt tcaagatgtt tgcgcaacta tttttggtag taatcccaaa gcggtgatct
     5641 a
//
LOCUS       DAZFLD010000247         5634 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.248, whole genome shotgun sequence.
ACCESSION   DAZFLD010000247 DAZFLD010000000
VERSION     DAZFLD010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5634
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.248"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..660
                     /locus_tag="ACHKBL_004300"
     CDS             <1..660
                     /locus_tag="ACHKBL_004300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309259.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5821077.1"
                     /translation="GSRIPSMQACLSRRSMVAMEGFVLYAGTNGLVSVDVNGNTALAT
                     EKIISPEQWQSQFNPASIVAYSWRGEYIACYTKPDGKQDVFVFSPVNMDIRYLSTPFD
                     CAWVDLAKDMMRVVTGDKMSVLAGGSLPSTIRWHSKIFSLPERTSFSCIRVKSPAPER
                     VGITIMADDVPVIHFAPGTFKGSVVRLPAATGQNWQVMVSGFGQVERITLSTSMSEMP
                     V"
     gene            657..1925
                     /locus_tag="ACHKBL_004301"
     CDS             657..1925
                     /locus_tag="ACHKBL_004301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HFO5821078.1"
                     /translation="MTRKPWRAGKDLSTVVENMEIGTGQRGDGRHAFVTREELVGLKL
                     ARRRTSGGASYALNPGIEIDSTLMTVDFPTKPLNFKATGGFGSVLLEWDMPNYRGHSL
                     TEIWRGTEDDLADAVLVATTPGQVYGDPVDPGWSGFYWIRFVNAAGVKGPWNAEKGTQ
                     AQTQIGVKAIIDQIRDEAAKSPVVSELRKEIKNAQGQAVKDAAIKTTEVVGTLREETT
                     RTIGGIETRISTLDSSTSESLNEVDKRITKLDKEGGEAFLAMWSKKAGVDGITAGIGI
                     VAGKDSEGRPVSQVAISASQLFVFDPNNPDNTAYPFAVSGGKVVIPKAMIYDAVIETL
                     VSRKVVADEVKAGVSITSPVIRSAVIQNGNFQVDSQGNLNIGGLFSVTSQGQLTIRYS
                     NQNVGLVIRNDKIEVYDQNGRLAVRIGRLR"
     gene            1940..2218
                     /locus_tag="ACHKBL_004302"
     CDS             1940..2218
                     /locus_tag="ACHKBL_004302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5821079.1"
                     /translation="MEYGFAIYNRNNVNVTGVLTPVFFLDRFTAESGSKTYTNKPDGK
                     SLQAVCCLFPWNNVFADRKVPKITINGNTVTWSNLEQGMGSYIYTFWG"
     gene            2224..2841
                     /locus_tag="ACHKBL_004303"
     CDS             2224..2841
                     /locus_tag="ACHKBL_004303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5821080.1"
                     /translation="MYGLSIMKPDGSVWISPGFTPQCLINKGTIPATEKSFFKTSIPS
                     GKSCFFFIRTEKKADVMYTHEQIDGYHALRLHVIVRGTNPGVTTVYAFANMVTPPSEY
                     GIAMYNPDGEMIYHGEMMLLDAKLIPVDIKFEKDLGYPCAIMPALVGYYNWKRTPYDR
                     PIYTTSTGATGNKIYSCEHYSGGATWDIRKPYIDKVLVINTSVYD"
     gene            2932..3666
                     /locus_tag="ACHKBL_004304"
     CDS             2932..3666
                     /locus_tag="ACHKBL_004304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFO5821081.1"
                     /translation="MKSIATLVVCAISGIACVNLSAHAAEGEHTISLGYAHFQFPGLK
                     DFVKDATAHNRETFSHFINRNYFSSLGEYTDGRVSGYEGKDKNPQGINIRYRYEITDD
                     FGVITSFTWTRSLTNSQTFIDVQSADHTRKIKNPAASARTDIRANYWSLLAGPSWRVN
                     QYMSLYAMAGIGVAKVTADLKIKDNINSSGGFSESNSTKKTSLAWAAGAQFNLNESVT
                     LDVAYEGSGSGDWRTSGVTAGIGLKF"
     gene            3899..4039
                     /locus_tag="ACHKBL_004305"
     CDS             3899..4039
                     /locus_tag="ACHKBL_004305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFO5821082.1"
                     /translation="MSQKSLITVTICMTVIFTIWMLHGSLCEFRLNLWGAEFAAFLQC
                     KQ"
     gene            4096..4497
                     /locus_tag="ACHKBL_004306"
     CDS             4096..4497
                     /locus_tag="ACHKBL_004306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5821083.1"
                     /translation="MAPVSGERMNDKILRYMQRVVRNSRNPEFMNEVKDACLKKQAFC
                     FEAPDGFLVLRSVLSDDGIPYVLVLLGVCTGSNSVERYLPEVKTLTRLAGGRWAEFHT
                     ARRGFIRLGKRLGFERMPDDEDGFMVFRIAV"
     gene            4591..5247
                     /locus_tag="ACHKBL_004307"
     CDS             4591..5247
                     /locus_tag="ACHKBL_004307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5821084.1"
                     /translation="MGGSKGGGDTKVKPTAAQIAQEEVAWKGWQDYQKILRPAEDNFM
                     EKVDDLNSEQQYDNIAGTTNLGYQKQFGEARKELAGNLAQSGVDPSSGRFNAVMNANQ
                     SDQVTGQIDTTTRGQVSQADKYVAGLQDVAALGSGQKADALQSFNSLADSSLAKAKSD
                     AQAAFTKQQGRASLVGAGLGAAGAYAMHKAGGSGGSGGAKTPGTGANAIQHQAQNWRL
                     "
     gene            5250..>5634
                     /locus_tag="ACHKBL_004308"
     CDS             5250..>5634
                     /locus_tag="ACHKBL_004308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5821085.1"
                     /translation="MEYGKYETLARYGYTGAARPQGDWQTSAALTRQQYDDWRTRYLP
                     RVARLADLGENNSLMNAQLARVGGLATSSLRTAQMAQDNQMARYGVSRPDNPDSNTLG
                     LRNALAIAGAKNGIREAEQDRQMNIL"
BASE COUNT         1468 a         1153 c         1597 g         1416 t
ORIGIN      
        1 ggctccagaa ttccttccat gcaggcatgc ctgagccgaa gaagtatggt ggcgatggag
       61 ggattcgtac tctatgccgg gacaaacggt ctggtatctg ttgatgtaaa cggtaataca
      121 gcactggcaa cggaaaagat tatttcacct gaacagtggc agagtcagtt taacccggcg
      181 tccattgtgg cttattcctg gcgtggtgaa tacattgcct gttacacgaa accggatggt
      241 aagcaggatg tgtttgtatt cagtccggtg aacatggata tccgttatct cagtacaccg
      301 tttgactgcg catgggttga tctcgcgaaa gatatgatgc gtgtggtgac aggagacaaa
      361 atgtcagtgc ttgccggggg ctctctgccc tccacgataa ggtggcattc aaaaattttt
      421 tcattacctg aaagaacctc tttttcctgt atcagggtga aatctccggc gcctgagcgg
      481 gtggggatca ccattatggc tgatgatgtt cctgtgattc attttgcgcc gggtacgttt
      541 aagggaagtg tggtgagact tccggcagca accgggcaaa actggcaggt gatggtatcc
      601 ggattcgggc aggtggaacg aataaccctg agtacatcga tgtcggagat gccggtatga
      661 ccagaaaacc gtggcgtgcg gggaaggatt tatccacagt tgtggagaac atggaaattg
      721 gcaccgggca gcgtggtgac ggacgccacg catttgtgac ccgtgaggaa ctggttggtc
      781 ttaaactcgc ccggcgtcga acatcgggtg gtgcctcata tgcactgaat ccgggtattg
      841 agattgacag tactttaatg actgttgatt ttcccacaaa accgctgaat tttaaggcga
      901 caggaggatt tggctcggtt cttcttgaat gggatatgcc taattatcgc ggacattcac
      961 tgactgaaat ctggcggggt acggaggatg accttgctga tgcagtgctg gttgccacga
     1021 cgccggggca ggtttacggc gatccggttg accctggctg gtcgggattt tactggatac
     1081 gttttgttaa cgcggcagga gtgaaaggtc catggaatgc tgaaaaaggc actcaggcac
     1141 aaacacagat cggcgtgaag gccatcattg accagatccg cgatgaggct gcaaagtcgc
     1201 cggttgtgtc cgagctgcgt aaagaaataa aaaacgcgca ggggcaggct gtaaaggatg
     1261 ctgcaattaa gacaaccgaa gttgtgggga ctctcaggga agaaacgaca agaacgattg
     1321 gtggtattga aacccgcatt agcacactgg attcgtcaac cagtgaatcg cttaatgagg
     1381 tcgacaagcg catcactaaa ctggataaag aaggcggtga ggcgtttctg gcaatgtggt
     1441 caaaaaaagc gggagttgat ggtatcactg cggggatcgg gattgtcgcc ggaaaagaca
     1501 gtgaaggcag gcctgtaagt caggttgcaa tttctgcgtc gcagttgttt gtctttgacc
     1561 cgaacaaccc ggataacacc gcctatccgt ttgcggtatc aggtggcaag gttgtgatcc
     1621 cgaaagcgat gatttatgac gcggtgattg aaacactggt gtcgcggaag gttgtggcgg
     1681 atgaggtaaa agccggggta agtatcactt cgccagttat ccggagtgcc gttattcaga
     1741 acggaaactt tcaggttgat tctcagggta acctgaatat tggaggcctt ttcagtgtta
     1801 cgtcacaagg gcaactgaca attcgttact ctaatcagaa tgtaggactg gtgatccgca
     1861 atgataaaat tgaggtttat gatcagaatg gacgactggc tgttcgcata ggcagattac
     1921 gctgatcagg aggtgagtat tggaatacgg ttttgccatt tataacagaa ataacgttaa
     1981 tgttacgggc gtgctgactc cggtattttt cctggacaga tttacagcgg agtctggctc
     2041 aaagacgtac acgaataaac ccgacgggaa atcattgcag gctgtatgtt gtttatttcc
     2101 ctggaataat gtatttgcgg atcggaaagt accgaagata accattaatg gcaatacggt
     2161 gacgtggtcg aatcttgagc agggtatggg atcttatatt tatacattct ggggataagt
     2221 gtcatgtatg gtttgagcat tatgaagccg gatggcagcg tatggataag tccaggtttt
     2281 acgccgcagt gtctgatcaa caaaggcacc ataccggcga ctgaaaagtc tttttttaaa
     2341 acatcaatcc cgtcaggcaa aagttgtttt ttctttatca gaacagagaa gaaggccgat
     2401 gtcatgtaca cgcatgaaca gattgatgga tatcatgcac taaggcttca tgtaattgtc
     2461 aggggaacga accctggtgt tacgacggtt tatgctttcg cgaatatggt tactccacct
     2521 tctgagtatg gtatcgccat gtataacccg gacggtgaga tgatttatca tggcgaaatg
     2581 atgctgcttg acgcgaagtt aatacctgtt gatatcaaat ttgaaaagga ccttggatat
     2641 ccatgcgcaa tcatgcctgc actggtcggg tattataact ggaaaagaac tccttatgat
     2701 cgaccgattt ataccacatc cactggtgct acaggaaata aaatatattc ctgtgagcat
     2761 tattccggtg gtgcaacatg ggatattcga aagccgtata tagataaggt cctggttatt
     2821 aatacatcag tatatgatta gttgaagcga gtctttaata ttcatttaaa atgtctaaaa
     2881 agatgtatta ttaaaaagtt tagcgtgtta tctgaataca ggatatctta aatgaagagt
     2941 atagcaacac tggttgtgtg tgcaatctcc gggattgcct gtgtaaattt atctgcacat
     3001 gcagcagaag gagagcatac aatttctctg gggtatgcgc actttcagtt tccgggactg
     3061 aaggattttg taaaggatgc gactgctcat aacagggaga ctttcagtca tttcatcaac
     3121 agaaactact tttcttcatt gggcgaatat acagatggtc gggtcagtgg atatgaaggc
     3181 aaggataaaa atccacaggg cattaatatc aggtatcgct acgagataac ggatgatttt
     3241 ggcgttatca cctcttttac atggacgcgt tctctcacta actcacagac atttattgat
     3301 gtgcagtcag ccgatcatac caggaagatt aagaatccgg cagcttctgc cagaacggat
     3361 atcagggcga attactggag tctgttagcg gggccttcat ggcgggttaa tcagtacatg
     3421 agtttatatg cgatggcagg gataggcgtg gctaaagtta ccgctgacct gaaaattaag
     3481 gacaatatta acagtagtgg cggattttct gaaagcaaca gcacgaaaaa aacctccctt
     3541 gcgtgggctg caggtgcaca gtttaacctg aatgagagtg ttacactgga tgtggcttac
     3601 gaaggttccg gctctggcga ctggcgcacg agtggcgtta ctgctggcat tggcctgaaa
     3661 ttctgacctg tatccggtaa ccatttacta cccgctgtga tggcgggttt tttattgccc
     3721 gtacagggca aaaatcgtaa attatgcgtg ggtgcctttc ggctgatggc tggagggtga
     3781 acctgaaggc ctgatgtgga aaggccccga gtcaacttaa cgttaacccg aggccctaac
     3841 acttcgtacc ttaagcaagt agaaggttag cgcctctctg taaaaggagt caagcgctat
     3901 gtcgcaaaaa tcgcttatca ccgtcacaat ttgtatgacg gttatcttca ccatctggat
     3961 gttacacggc tcgttgtgtg agttccggct gaatttgtgg ggagcggagt ttgcggcgtt
     4021 cttacagtgt aagcagtagg aaaaccgcgg cggggacgaa agtccccgtc aactggttgc
     4081 tgaggttcag ccgatatggc acccgtttca ggtgagagaa tgaacgataa aattctccgg
     4141 tatatgcagc gtgtggtgag aaattcccgc aaccctgaat ttatgaatga agttaaagac
     4201 gcctgcctta aaaagcaggc gttttgtttt gaggctcctg atggctttct ggtgctgcgt
     4261 tctgtgctca gtgacgatgg tatcccttat gttctggtgt tgctgggcgt gtgtacgggg
     4321 agtaacagcg ttgagcgtta tctgccggag gtgaagacat taacccgtct ggctggcgga
     4381 cgctgggctg aatttcatac ggcaaggcgg ggatttatcc ggctgggaaa acgtctgggc
     4441 tttgagcgaa tgccggatga tgaggatggc ttcatggtgt tcaggatagc ggtctgactg
     4501 ctacagtttt catcattgtg tttaaaccaa cattgtaatt cacattctga ccctgctccg
     4561 gcagggtttt ttgttatcca gggggccatt atgggtggaa gtaaaggcgg tggtgatacc
     4621 aaagtaaaac caacagcagc gcaaatagca caggaagaag tggcctggaa agggtggcag
     4681 gattatcaaa aaatcctccg cccggctgaa gataacttca tggaaaaggt cgatgatctg
     4741 aacagtgagc agcagtacga caatattgct ggcaccacaa atctggggta tcagaaacag
     4801 tttggcgaag cacggaagga gctggcgggt aatcttgctc agtccggtgt tgacccgtcc
     4861 agtggtcgtt ttaacgcggt aatgaatgcg aaccagagtg atcaggtaac cgggcagatt
     4921 gacacaacca cacgggggca ggtatcgcag gcagataagt atgttgccgg gctacaggat
     4981 gttgcagctc tcggttctgg tcagaaggcg gatgcgttac agagttttaa ctcgctggca
     5041 gacagcagtc tggcaaaagc taaatcggac gcacaggctg cgtttacgaa acagcagggg
     5101 cgagcctctc ttgttggcgc tggtttgggt gcggcaggtg catatgcgat gcataaggct
     5161 ggcggtagcg gaggaagtgg cggtgctaaa acacctggca ccggcgctaa tgccattcag
     5221 catcaggctc agaactggag actgtgatta tggagtacgg taaatacgaa acacttgcga
     5281 gatacggtta taccggagct gcccgtccac agggtgactg gcagacgtca gcagcgctga
     5341 cacgccaaca atacgacgac tggcgaacca gatatttgcc ccgcgtggca aggctggctg
     5401 accttggcga gaacaacagc ctgatgaatg cacagcttgc ccgggtgggc ggccttgcca
     5461 cttccagtct ccgtacagcg cagatggcgc aggataacca gatggcgaga tacggggtaa
     5521 gccgcccgga taatcccgac agtaatacgc tggggttacg taatgccctg gcaattgctg
     5581 gcgcgaaaaa tggtatccgt gaagccgaac aggatcgcca gatgaatatt ctga
//
LOCUS       DAZFLD010000248         5600 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.249, whole genome shotgun sequence.
ACCESSION   DAZFLD010000248 DAZFLD010000000
VERSION     DAZFLD010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5600
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.249"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(36..773)
                     /gene="bamD"
                     /locus_tag="ACHKBL_004309"
     CDS             complement(36..773)
                     /gene="bamD"
                     /locus_tag="ACHKBL_004309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HFO5821086.1"
                     /translation="MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQ
                     DGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYYKNADLPLAQAAIDRFIRLNPTH
                     PNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
                     TDATKRLVFLKDRLAKYEYSVAEYYTERGAWVAVVNRVEGMLRDYPDTQATRDALPLM
                     ENAYRQMQLNAQAEKVAKIIAANSSNT"
     gene            908..1888
                     /gene="rluD"
                     /locus_tag="ACHKBL_004310"
     CDS             908..1888
                     /gene="rluD"
                     /locus_tag="ACHKBL_004310"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HFO5821087.1"
                     /translation="MAQRVQLTATVSENQLGQRLDQALAEMFPDYSRSRIKEWILDQR
                     VLVNGKVCDKPKEKVLGGEQVAINAEIEEEARFEPQDIPLDIVYEDEDIIVINKPRDL
                     VVHPGAGNPDGTVLNALLHYYPPIADVPRAGIVHRLDKDTTGLMVVAKTVPAQTRLVE
                     SLQRREITREYEAVAIGHMTAGGTVDEPISRHPTKRTHMAVHPMGKPAVTHYRIMEHF
                     RVHTRLRLRLETGRTHQIRVHMAHITHPLVGDPVYGGRPRPPKGASEAFISTLRKFDR
                     QALHATMLRLYHPISGIEMEWHAPIPEDMVELIEVMRADFEEHKDEVDWL"
     gene            1885..2616
                     /gene="yfiH"
                     /locus_tag="ACHKBL_004311"
     CDS             1885..2616
                     /gene="yfiH"
                     /locus_tag="ACHKBL_004311"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphorylase YfiH"
                     /protein_id="HFO5821088.1"
                     /translation="MSKLIVPQWPLPKGVAACSSTRIGGVSLPPYDSLNLGAHCGDNP
                     DHVEENRKRLFAAGNLPSKPVWLEQVHGKDVLKLTGEPYASKRADASYSNTPGTVCAV
                     MTADCLPVLFCNRAGTEVAAAHAGWRGLCSGVLEETVSCFADNPENILAWLGPAIGPR
                     AFEVGAEVREAFMAVDAKASTAFIQHGDKYLADIYLLARQRLANVGVEQIFGGDRCTY
                     TENETFFSYRRDKTTGRMASFIWLI"
     gene            2746..5319
                     /gene="clpB"
                     /locus_tag="ACHKBL_004312"
     CDS             2746..5319
                     /gene="clpB"
                     /locus_tag="ACHKBL_004312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HFO5821089.1"
                     /translation="MRLDRLTNKFQLALADAQSLALGHDNQFIEPLHLMSALLNQEGG
                     SVSPLLTSAGINAGQLRTDINQALNRLPQVEGTGGDVQPSQDLVRVLNLCDKLAQKRG
                     DNFISSELFVLAALESRGTLADILKAAGATTANITQAIEQMRGGESVNDQGAEDQRQA
                     LKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAKQEGNVILF
                     IDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQ
                     KVFVAEPSVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAID
                     LIDEAASSIRMQIDSKPEELDRLDRRIIQLKLEQQALMKESDEASKKRLDMLNEELSD
                     KERQYSELEEEWKAEKASLSGTQTIKAELEQAKIAIEQARRVGDLARMSELQYGKIPE
                     LEKQLEAATQLEGKTMRLLRNKVTDAEIAEVLARWTGIPVSRMMESEREKLLRMEQEL
                     HHRVIGQNEAVDAVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFD
                     SDEAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKA
                     HPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSDLIQERFGELDYAHMKEL
                     VLGVVSHNFRPEFINRIDEVVVFHPLGEQHIASIAQIQLKRLYKRLEERGYEIHISDE
                     ALKLLSENGYDPVYGARPLKRAIQQQIENPLAQQILSGELVPGKVIRLEVNEDRIVAV
                     Q"
BASE COUNT         1385 a         1346 c         1521 g         1348 t
ORIGIN      
        1 gctcaagggc tgccgttttg tgtttcaggt ttctgttatg tattgctgct gtttgcggcg
       61 atgattttcg ctactttttc agcttgcgca ttcaactgca tctgacggta tgcattttcc
      121 atcagcggca gcgcatcacg cgtagcctgg gtatccgggt agtcgcgcaa catgccttct
      181 acgcggttaa cgacggcaac ccatgcacca cgttctgtat agtactcggc aacggagtat
      241 tcatatttcg ccagacgatc tttcaggaat accagacgtt tggtggcatc ggtggtgtac
      301 tgactgttcg gatagccgcg caccagtttg gaaaagtcac taaacgcagc tcgtgcatgt
      361 tgaggatcgc gatcgctacg gtcaacgcca aagaaccctt gcagcgcact gtcatccagc
      421 gccatattgg tcaggccacg catgtacatg acataatcga tattcggatg ggtcgggtta
      481 aggcgaataa aacgatcgat ggcagcctgt gctaacggca aatcggcgtt tttatagtag
      541 gcgtagatga gatccagctg cacctgctgc gaatacggac caaacggata gcgattatct
      601 aacgcttcca gttgcgttat tgcctgtctc cagttaccgt cctgcagctt ttgttgtgca
      661 gtcgcgtaaa tttcatttgg cggattatca ggtacttctt cctttgaccc cgagcaaccc
      721 gccaaaaaca ggcttagtgt ggcggctgcc accagatatt tcatgcgcgt catgacgttt
      781 tgactttcct caaaatgtaa tacgggagat tctctgttcc tgctcccggt taagaccagc
      841 tacaatagca cactatatta aacggcaaag ccgtaaaacc ccaacgataa acgaagaagc
      901 agtatatatg gcacaacgag tacagctcac tgcaacggtg tccgaaaacc aactcggtca
      961 acgcttagat caggctttgg ccgaaatgtt cccggattat tcacgttcgc gaataaaaga
     1021 atggatcctc gaccagcgcg tgctggttaa cggcaaagtt tgtgataagc cgaaagaaaa
     1081 agtattgggt ggcgagcagg tggccatcaa cgctgagatt gaagaagaag cgcgttttga
     1141 accgcaggat atcccgctgg atatcgtcta tgaagatgaa gatatcatcg tcattaataa
     1201 accacgcgac ctggtggtac atcctggcgc gggtaacccg gatggtacgg tactgaatgc
     1261 gttgcttcat tattatccgc ccattgccga tgtaccgcgt gcgggcatcg tccatcgtct
     1321 ggataaagac accaccggcc tgatggttgt ggcaaaaacc gttccggctc agacgcgttt
     1381 agtcgaatct ttgcaacggc gtgaaattac tcgtgagtat gaagcggtgg caattggtca
     1441 tatgaccgct ggcggtacag tagatgaacc aatcagccgc cacccgacca aacgtactca
     1501 catggcggtg catccgatgg gtaaaccggc ggtcactcac tatcgcatca tggaacactt
     1561 ccgtgtgcac acgcgtctgc ggttgcgtct ggaaaccgga cgtacgcacc agatccgcgt
     1621 gcatatggcc catatcactc atccgctggt gggcgatccg gtttatggtg gccgtccgcg
     1681 tccgccaaaa ggtgcttcgg aagcgtttat ctccacgctg cgtaagtttg accgccaggc
     1741 gcttcatgca accatgctgc gtctttatca cccgatctcc ggtatcgaaa tggaatggca
     1801 tgcgcctatt ccagaagata tggtggagct gattgaggtg atgcgcgccg atttcgaaga
     1861 gcataaggat gaagtggact ggttatgagt aagctgattg tcccgcagtg gccgctgcca
     1921 aaaggtgttg cggcctgtag ctccactcgt atcggcggcg tgagcttgcc cccgtatgat
     1981 tcgctcaacc tcggtgctca ttgtggcgat aacccggatc acgttgaaga gaatcgtaag
     2041 cgactttttg ctgcgggcaa tttgccttca aaaccggtct ggcttgagca ggtacacggc
     2101 aaagatgtgc ttaagcttac tggcgaacct tatgcctcaa aacgagcaga tgcctcttat
     2161 agcaacacgc cgggcacggt ttgcgcagtg atgactgccg actgcctccc cgtgctgttt
     2221 tgcaatcgag cgggaacgga agtcgccgcc gctcatgctg gctggcgtgg actgtgctca
     2281 ggcgtgctgg aagagacggt ttcctgtttt gctgataatc cggaaaatat tctcgcctgg
     2341 ttagggccgg caattggtcc acgcgcgttt gaagtggggg cggaggttcg cgaggcgttt
     2401 atggcagtag acgccaaagc gagtacagct ttcattcagc atggtgataa gtatctggcg
     2461 gatatttatc tccttgcccg gcagcgtctg gcgaacgtcg gtgtcgaaca aattttcggc
     2521 ggtgaccgtt gtacatatac ggaaaatgag actttcttct cttatcgtcg cgacaagacc
     2581 accggtcgta tggcaagttt catttggctg atataaccta aagaatcaag acgatccggt
     2641 acgcgtgatt ttcttttcac attaatctgg tcaataacct tgaataattg agggatgacc
     2701 tcatttaatc tccagtagca actttgatcc gttatgggag gagttatgcg tctggatcgt
     2761 cttactaata aattccagct tgctcttgcc gatgcccaat cacttgcact cgggcacgac
     2821 aaccaattca tcgaaccact tcatttaatg agcgccctgc tgaatcagga agggggttcg
     2881 gttagtcctt tattaacatc cgctggcatt aatgctggcc agttgcgcac agatatcaat
     2941 caggcattaa atcgtttacc acaggttgaa ggtactggtg gtgatgtcca gccatcacag
     3001 gatctggtgc gcgttcttaa tctttgcgac aagctggcgc aaaaacgtgg tgataacttt
     3061 atctcgtcag aactgttcgt tctggcggca cttgagtctc gcggcacgct ggccgacatc
     3121 ctgaaagcag caggggcgac caccgccaac attactcaag cgattgaaca aatgcgtgga
     3181 ggtgaaagcg tgaacgatca aggtgctgaa gaccaacgtc aggctttgaa aaaatatacc
     3241 atcgacctta ccgaacgagc cgaacagggc aaactcgatc cggtgattgg tcgtgatgaa
     3301 gaaattcgcc gtaccattca ggtgctgcaa cgtcgtacta aaaataaccc ggtactgatt
     3361 ggtgaacccg gtgtcggtaa aactgccatc gttgaaggtc tggcgcagcg tattatcaac
     3421 ggcgaagtgc cggaagggtt gaaaggccgc cgcgtactgg cgctggatat gggcgcgctg
     3481 gtggctgggg cgaaatatcg cggtgagttt gaagaacgtt taaaaggcgt gcttaacgat
     3541 cttgccaaac aggaaggcaa cgtcatcctg tttatcgacg aattacatac catggtcggt
     3601 gcgggtaaag ccgatggcgc aatggacgcc ggaaacatgc tgaaaccggc gctggcgcgt
     3661 ggtgaattgc actgcgtagg tgccacgacg cttgacgaat atcgccagta catcgaaaaa
     3721 gatgctgcgc tggagcgtcg tttccagaaa gtgtttgttg ccgagccttc tgttgaagac
     3781 accattgcta ttctgcgtgg cttaaaagaa cgttacgaac tgcaccacca tgtgcaaatt
     3841 actgacccgg caattgttgc agcggcgacg ttgtctcatc gctacattgc tgaccgtcag
     3901 ctgccagata aagccatcga cctgatcgat gaggcagcat ccagcattcg tatgcagatt
     3961 gactcaaaac cagaagaact cgaccgactc gatcgccgta tcattcagct caaactggaa
     4021 caacaggcgt taatgaaaga gtctgatgaa gccagtaaaa aacgcctgga tatgctcaac
     4081 gaagaactga gcgacaaaga acgtcagtac tccgagttag aagaagagtg gaaagcagag
     4141 aaagcatcgc tttctggtac acagaccatt aaagcggaac tggaacaggc gaaaatcgct
     4201 attgaacagg ctcgccgtgt gggggacctg gcgcggatgt ctgaactgca atacggcaaa
     4261 atcccggaac tggaaaagca actggaagcc gcaacgcagc tcgaaggcaa aactatgcgt
     4321 ctgttgcgta ataaagtgac cgatgccgaa attgctgaag tgctggcgcg ttggacgggg
     4381 attccggttt ctcgcatgat ggaaagcgag cgcgaaaaac tgctgcgtat ggagcaagaa
     4441 ctgcaccatc gcgttattgg tcagaacgaa gcggttgatg cggtatctaa cgctattcgt
     4501 cgtagccgtg cggggctggc ggatccaaat cgcccgattg gttcattcct gttcctcggc
     4561 ccaaccggtg tggggaaaac tgagctttgt aaggcgctag cgaactttat gtttgatagc
     4621 gacgaggcga tggtccgtat cgatatgtcc gagtttatgg agaaacactc ggtgtctcgt
     4681 ctggttggtg cgcctccggg atatgtcggt tatgaagaag gtggctacct gaccgaagct
     4741 gtgcgtcgtc gtccgtattc cgtcatcctg ctggatgaag tggaaaaagc gcatccggat
     4801 gtcttcaaca ttctgttgca ggtactggat gatgggcgtc tgactgacgg gcaggggaga
     4861 acggtcgact tccgtaatac ggttgtcatt atgacctcta acctcggttc cgatttgatt
     4921 caggaacgct tcggtgaact ggattatgcg cacatgaaag agctggtgct cggtgtggta
     4981 agccataact tccgtccgga attcattaac cgtatcgatg aagtggtggt cttccatccg
     5041 ctgggtgaac agcacattgc gtcgattgcg cagattcagt tgaaacgtct gtacaaacgt
     5101 ctggaagaac gtggttatga aatccacatt tctgacgagg cgctgaaact gctgagcgag
     5161 aacggttacg atccggtcta tggtgcacgt ccgctgaaac gtgctatcca gcagcagatc
     5221 gaaaacccgc tggcacagca aatactgtct ggtgaattgg ttccgggtaa agttattcgc
     5281 ctggaagtta atgaagaccg gattgtcgcc gtccagtaat gataaaacga gcccttcggg
     5341 gctcgttttt gtctataaat tagacggaaa agactatgtt taagatgttt tgcctgaaaa
     5401 atgagcgaac gataaagttt ttatattttt tgcttgtcag gccggaataa ctccctataa
     5461 tgcgccacca ctgacacgga acaacggcaa acacgccgcc gggttagcgg ggttctcctg
     5521 agaattccgg cagagaaagc aaaaacaaat gcttgactct gtagcgggaa agcgtattat
     5581 gcacaccccg cgccgctgag
//
LOCUS       DAZFLD010000249         5546 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.250, whole genome shotgun sequence.
ACCESSION   DAZFLD010000249 DAZFLD010000000
VERSION     DAZFLD010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5546
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.250"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(67..2136)
                     /gene="glyS"
                     /locus_tag="ACHKBL_004313"
     CDS             complement(67..2136)
                     /gene="glyS"
                     /locus_tag="ACHKBL_004313"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HFO5821090.1"
                     /translation="MSEKTFLVEIGTEELPPKALRSLAESFAANFTAELDNAGLAHGT
                     VQWFAAPRRLALKVANLAEAQPDREIEKRGPAIAQAFDAEGKPSKAAEGWARGCGITV
                     DQAERLTTDKGEWLLYRAHVKGESTEALLPNMVATSLAKLPIPKLMRWGASDVHFVRP
                     VHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGEPEFTIDNADQYPEILRERGKVIAD
                     YEERKAKIKADAEEAARKIGGNADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPSEA
                     LVYTMKGDQKYFPVYANDGKLLPNFIFVANIESKDPQQIISGNEKVVRPRLADAEFFF
                     NTDRKKRLEDNLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDDLPSNPVAC
                     ALAIADKMDTLAGIFGIGQHPKGDKDPFALRRAALGVLRIIVEKNLNLDLQTLTEEAV
                     RLYGDKLTNTNVVDDVIDFMLGRFRAWYQDEGYTVDTIQAVLARRPTRPADFDARMKA
                     VSHFRTLDAAASLAAANKRVSNILAKSDEVLSDRVNASTLKEPEEIKLAMQVVVLRDK
                     LEPYFAEGRYQDALVELAELREPVDAFFDKVMVMVDDKELRINRLTMLEKLRELFLRV
                     ADISLLQ"
     gene            complement(2146..3057)
                     /gene="glyQ"
                     /locus_tag="ACHKBL_004314"
     CDS             complement(2146..3057)
                     /gene="glyQ"
                     /locus_tag="ACHKBL_004314"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HFO5821091.1"
                     /translation="MQKFDTRTFQGLILTLQDYWARQGCTIVQPLDMEVGAGTSHPMT
                     CLRALGPEPMAAAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLGSL
                     KELGMDPTIHDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPVT
                     GEITYGLERLAMYIQGVDSVYDLVWSDGPLGKTTYGDVFHQNEVEQSTYNFEYADVDF
                     LFTCFEQYEKEAQQLLALENPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILRI
                     RTLTKAVAEAYYASREALGFPMCNKDK"
     gene            complement(3152..3460)
                     /gene="ysaB"
                     /locus_tag="ACHKBL_004315"
     CDS             complement(3152..3460)
                     /gene="ysaB"
                     /locus_tag="ACHKBL_004315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsaB family lipoprotein"
                     /protein_id="HFO5821092.1"
                     /translation="MMMNSFFPAMALMVLMALVGCSTPPPVQKAQRVKVDPLRSLNME
                     ALCKDQAAKRYNTGEQKIDVTAFEQFQGSYEMRGYTFRKEQFVCSFDADGHFLHLSMR
                     "
     gene            3634..4629
                     /gene="wecH"
                     /locus_tag="ACHKBL_004316"
     CDS             3634..4629
                     /gene="wecH"
                     /locus_tag="ACHKBL_004316"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyltransferase WecH"
                     /protein_id="HFO5821093.1"
                     /translation="MQPKIYWIDNLRGIACLMVVMIHTTTWYVTNAHSVSPVTWDIAN
                     VLNSASRVSVPLFFMISGYLFFGERSAQPRHFLRIGLCLLFYSAIALLYIALFTSINV
                     ELALKNLLQKPVFYHLWFFFAIAVIYLVSPLIQVKNVGGKMLLVLMVVIGIIANPNTV
                     PQKIDGFEWLPINLYINGDTFYYILYGMLGRAIGMMDTQHKALSWVCAALFATGVFII
                     SRGTLYELQWRGNFADTWYLYCGPMVFICAITLLTLVKNTLDTRTVRGLGLISRHSLG
                     IYGFHALIIHALRTRGIELKNWPILDIIWIFCATLAASLLLSMLVQRIDRNRLVS"
     gene            complement(4671..5108)
                     /gene="yiaA"
                     /locus_tag="ACHKBL_004317"
     CDS             complement(4671..5108)
                     /gene="yiaA"
                     /locus_tag="ACHKBL_004317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YiaA"
                     /protein_id="HFO5821094.1"
                     /translation="MDNKISTYSPAFSIVSWIALVGGIVTYLLGLWNAEMQLNEKGYY
                     FAVLVLGLFSAASYQKTVRDKYEGIPTTSIYYMTCLTVFIISVALLMVGLWNATLLLS
                     EKGFYGLAFFLSLFGAVAVQKNIRDAGINPPKETQVTQEEYSE"
     gene            complement(5153..5494)
                     /gene="yiaB"
                     /locus_tag="ACHKBL_004318"
     CDS             complement(5153..5494)
                     /gene="yiaB"
                     /locus_tag="ACHKBL_004318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YiaB"
                     /protein_id="HFO5821095.1"
                     /translation="MKTSKTVAKLLFVVGALVYLVGLWISCPLLSGKGYFLGILMTAT
                     FGNYAYLRAEKLGQLDNFFTHICQLVALITIGLLFIGVLNAPINAYEMVIYPIAFFVC
                     LFGQMRLFRSA"
BASE COUNT         1350 a         1435 c         1360 g         1401 t
ORIGIN      
        1 aaggttgctc tattatttag cgataaaaaa gcctgccaga tggcaggctt tttaataacg
       61 gcgttattac tgtaacagcg aaatatccgc aacgcgcagg aataattcgc gcagtttctc
      121 cagcatggta agacggttga ttcgcaattc tttgtcatca accatgacca tcactttatc
      181 gaagaaggca tcaaccggtt cacgcaattc agccagttcg accagcgcgt cctggtaacg
      241 accttcagcg aagtacggct ccagcttgtc gcgcagcacc acaacttgca tcgccagttt
      301 aatttcttcc ggctctttca gagtagaagc attcacgcga tcgctcagca cttcgtcaga
      361 cttcgccaga atgttagata cacgcttgtt tgccgccgcc agtgacgctg ccgcatccag
      421 agtacggaag tgcgataccg ccttcatacg ggcatcgaaa tctgccggac gagtcggacg
      481 acgcgccagt accgcctgga tggtatcaac ggtgtaacct tcgtcctgat accaggcgcg
      541 gaagcgaccg agcataaagt cgataacatc atcaactacg ttggtattag tcagcttatc
      601 gccatacaga cgcaccgctt cttcggtcag cgtttgcaga tcaaggttga ggttcttctc
      661 aacgataata cgcagcacgc caagtgcggc acgacgcaac gcaaacgggt ctttgtcgcc
      721 tttcggatgc tggccgatac cgaagatacc cgccagagta tccatcttgt cagcaatcgc
      781 cagcgcacag gctaccgggt tagacggcag gtcatcaccg gcaaagcgcg gctgatactg
      841 ctcgttcagc gccacggcaa catcttccgc ttcgccatcg tgacgcgcgt agtgcatccc
      901 cataacgccc tgggtgtcgg tgaactcgaa gaccatgttg gtcatcaggt cgcacttgga
      961 cagcaggccc gcacgggtag cgtggttaac gtcagcgcca atctgttcag caatccagcc
     1021 agccagcgcc tggatgcggt cagtcttgtc acgcagcgta cccagctgtt gctggaacag
     1081 cacggtttgc aggcgcggca ggttatcttc cagacgtttt ttacggtcgg tgttgaagaa
     1141 gaactcggca tccgccagac gtggacgaac gactttctcg ttaccagaga taatttgctg
     1201 cggatctttc gattcgatgt tggcaacgaa gataaagttc ggcagcagtt tgccgtcgtt
     1261 cgcatacacc gggaagtatt tctggtcacc tttcatggtg taaaccagcg cttcagacgg
     1321 caccgcgagg aatttctctt cgaattttgc ggtcagcaca accggccatt ccaccagcga
     1381 agcgacttct tccaacaggc tttcgcttaa gtcagcgtta ccgccaatct tacgcgccgc
     1441 ttcttcggca tcggctttaa tcttcgcctt acgttcttcg taatcggcga tgactttccc
     1501 gcgctcacgc agaatttccg gatactgatc agcattgtcg atagtgaatt ccggctcgcc
     1561 cataaagcgg tggccgcgaa tcacgcgatc ggactgaatg cccagaatgg tagccggaat
     1621 aactttgtcg cccagcagca gggtcacggt gtgcaccgga cgcacgaagt gcacgtcgct
     1681 tgcaccccaa cgcatcagtt tcgggatcgg cagtttcgcc agcgaagtcg caaccatatt
     1741 cggcagcagt gcttcagtgc tttcgccctt cacgtgggcg cgatacagca gccattcgcc
     1801 tttatcggta gtcagacgct cagcctggtc aacggtaata ccgcaaccac gcgcccagcc
     1861 ttctgccgct ttgctcggtt tgccttcagc gtcgaacgcc tgggcaatcg ccgggccgcg
     1921 tttttcgatt tcacgatccg gttgcgcttc cgccaggtta gctactttaa gcgccagacg
     1981 acgcggagca gcaaaccatt gaacggtgcc gtgtgcgagg ccagcgttat ccagctccgc
     2041 agtaaagttc gcagcaaagg actcagccag gctgcgcagt gcttttggtg gcagctcttc
     2101 agtgccgatt tccaccagaa aagttttctc agacatagcc gcctcttact tatctttgtt
     2161 gcacatcggg aagccgaggg cttcacggga agcgtagtat gcttctgcca ctgctttggt
     2221 cagggtgcga atgcgcagaa tatagcgctg acgctcggtg acggagatgg ctttacgcgc
     2281 atccagcagg ttgaagctgt gggcggcttt cagaatacgc tcgtaggctg gcagcggcag
     2341 cggattttcc agcgccagca gctgctgcgc ttctttctcg tactgctcaa agcaggtgaa
     2401 caggaagtcc acatccgcgt attcgaagtt gtaagtggac tgctccactt cgttctgatg
     2461 gaacacgtcg ccataggtgg ttttacccag cgggccgtcg ctccagacca ggtcgtaaac
     2521 gctgtctacg ccctgaatgt acattgccag acgttccaga ccgtaggtga tctcgccggt
     2581 aaccggttta cactccagac caccaacctg ctggaagtaa gtgaactgcg tcacttccat
     2641 gccgttcagc cacacttccc agcccagccc ccaggcaccc agcgtcgggt tttcccagtt
     2701 atcttccacg aaacggatgt cgtggatggt cgggtccatg cccagctctt tcagagaacc
     2761 aaggtacagc tcctgaatat tgtccggcga tggcttaatg accacctgga actgatagta
     2821 gtgctgtaaa cggttggggt tttcgccgta gcgaccatcg gtcgggcgac gagaaggctg
     2881 aacataagca gccgccatcg gttctggccc cagcgcgcgc agacaggtca ttgggtgaga
     2941 ggttcccgcg ccgacttcca tgtccaatgg ttgaacaatg gtgcagccct ggcgagccca
     3001 gtaatcctgt aaggtcagga tcaagccctg gaaggtcctg gtatcaaact tttgcatatt
     3061 atttcgtgct ggatacgtgt ggatttaaag gaagggatca gtatacccgc tggatggaag
     3121 atatacagta cgaaacggga aaaagcaggg cttaacgcat ggaaagatgc aaaaaatggc
     3181 catctgcgtc aaaagaacag acaaactgct ctttacggaa ggtataaccg cgcatttcgt
     3241 agcttccctg gaactgttca aatgcggtga cgtcgatttt ttgctcgccg gtgttataac
     3301 gtttcgccgc ctgatccttg cataacgctt ccatattcaa cgaacgcaga ggatcaacct
     3361 ttacccgttg tgctttctgc acgggtggcg gtgtagaaca acccactagc gccataagca
     3421 ccataagcgc cattgccgga aagaaagagt tcatcatcat taaattaccg tctggtttgc
     3481 aggtgagtct tattatttat atcggtatag tggctgatat cttacgaatt tccttaagcc
     3541 tctataagct gtagtgataa aactcagatt attccagtgc gccaaccgaa tgatttatcg
     3601 gtcagacttt tttaaccaga tacagagaca cagatgcagc ccaaaattta ctggattgat
     3661 aacctgcgag ggatagcgtg tttaatggtg gtgatgattc acaccactac ctggtatgtg
     3721 accaatgctc atagtgttag ccccgtcacc tgggatatcg ccaatgttct gaattctgcc
     3781 tctcgtgtca gcgtgccgct atttttcatg atttccggct atctcttttt tggcgaacgc
     3841 agcgcccagc cgcgccattt cttgcgtatc ggcttatgtc tgctttttta tagcgcaatc
     3901 gcactgctct acattgcgct gtttacctcc atcaatgtgg agttagcgct gaaaaacctg
     3961 ctgcaaaagc cagtgtttta ccacttatgg tttttcttcg cgattgcggt gatttatctg
     4021 gtttcaccgc tgattcaggt gaagaacgtc ggcggaaaaa tgttgctggt gctaatggtg
     4081 gtgattggta ttatcgctaa cccaaacaca gtgccgcaga aaattgacgg ttttgaatgg
     4141 ctgccaatta acttatatat caatggcgat actttttact acatcctgta tggcatgttg
     4201 ggccgcgcta tagggatgat ggacacacag cataaagcac tgtcgtgggt gtgcgcagca
     4261 ctgtttgcga cgggggtttt tattatctct cgcgggacat tatatgaatt gcagtggcgc
     4321 ggaaattttg ccgatacctg gtatctttac tgtgggccga tggtttttat ctgcgcaatc
     4381 acgctattga ctctggttaa aaacacgctt gatacgcgta ccgttcgcgg acttggctta
     4441 atctcccgcc attcgttggg catatacggg ttccacgcct tgattatcca tgcactgcgc
     4501 acccggggga ttgagcttaa aaactggcca atactcgata ttatttggat cttttgcgcg
     4561 acgttggcag cgagtttgtt actttctatg ctggtacaac gaatcgacag aaacagacta
     4621 gtgagttaag taaaaggccg gtcacattgg actgaccggg cttatgtgaa ttattcgctg
     4681 tattcttcct gggtaacctg tgtttctttt ggtgggttta ttccggcatc acgaatattc
     4741 ttctgcaccg ctactgcacc aaacaagctt aagaagaaag ccagtccata aaaacctttc
     4801 tcgctgagta ataacgttgc attccacaga cctaccatca gtaacgcaac agagataata
     4861 aagacagtca ggcaggtcat ataataaatg gaggtggtcg gtatgccttc atacttgtcc
     4921 cgaacggtct tttgataaga cgccgcagaa aacagtccta ataccagtac ggcaaaataa
     4981 tatccttttt catttaactg catctctgca ttccatagcc ctaacagata ggtaacgata
     5041 ccaccaacga gagctatcca tgacacaata ctgaatgccg gagaataggt tgatattttg
     5101 ttgtccatca cagtattcct tcattcctga atgtgtaaga gctttatgtt gctcatgccg
     5161 agcgaaacaa acgcatttga ccaaacaagc agacaaaaaa ggcgataggg tagatcacca
     5221 tttcataagc attgataggt gcgtttaaaa caccgataaa caagagaccg atagtgatta
     5281 acgcaactaa ctggcagata tgggtaaaaa aattatccag ttgcccgagt ttttctgcgc
     5341 gaagatatgc atagttgcca aaagttgctg tcattaatat gccaagaaaa tagccttttc
     5401 cacttaacaa tgggcatgag atccatagcc caaccagata aaccagcgcc ccgacaacaa
     5461 ataatagttt tgccacagtc tttgaggttt tcataacgat ctccatatct accagcgata
     5521 cattacgagt aaccaacgaa agacaa
//
LOCUS       DAZFLD010000250         5481 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.252, whole genome shotgun sequence.
ACCESSION   DAZFLD010000250 DAZFLD010000000
VERSION     DAZFLD010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5481
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.252"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..250)
                     /locus_tag="ACHKBL_004319"
     CDS             complement(<1..250)
                     /locus_tag="ACHKBL_004319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFO5821096.1"
                     /translation="MILHAQAKHGKPGLPWLVFLHGFSGDCHEWQEVGEAFADYSRLY
                     VDLPGHGGSAAISVDGFDDVTDLLRKTLVSYNILNFWLV"
     gene            complement(247..1917)
                     /gene="menD"
                     /locus_tag="ACHKBL_004320"
     CDS             complement(247..1917)
                     /gene="menD"
                     /locus_tag="ACHKBL_004320"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070204 -
                     2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-
                     carboxylic-acid synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HFO5821097.1"
                     /translation="MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAEN
                     SAFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLIL
                     LTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTQDIPARWLVSTIDHALGTLH
                     AGGVHINCPFAEPLYGEMDDTGLSWQQRLGDWWQDDKPWLREAPRLESEKQRDWFFWR
                     QKRGVVVAGRMSAEEGKKVALWAQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSEL
                     QQAQIVVQLGSSLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAHHRGRRLIANIADWL
                     EQHPAEKRQPWCVEIPRLAEQAMQAVIARRDAFGEAQLAHRISDYLPEQGQLFVGNSL
                     VVRLIDALSQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLN
                     ALALLRQVSAPLVLIVVNNNGGQIFSLLPTPKNERERFYLMPQNVHFEHAAAMFELKY
                     HRPQNWQELETALADAWRTPTTTVIEMVVNDTDGAQTLQQLLAQVSHL"
     gene            complement(2006..3301)
                     /gene="menF"
                     /locus_tag="ACHKBL_004321"
     CDS             complement(2006..3301)
                     /gene="menF"
                     /locus_tag="ACHKBL_004321"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HFO5821098.1"
                     /translation="MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWL
                     ASQQTYPQFYWQQRNGDEEAAVLGAITRFTSLDQAQTFLRQHPEHADLRIWGLNAFDP
                     SQGNLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIKAKEFIATLVSIKPLPGLHLTT
                     TREQHWPDKTGWTQLIELATKTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLN
                     LNCYHFYMAFDGEIAFLGSSPERLWRRRDKALRTEALAGTVANHPDDKQAQQLGEWLM
                     ADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLRRCIWTALNKADDVI
                     CLHQLQPTAAVAGLPRDLARQFIARHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKIS
                     GNVVRLYAGAGIVRGSDPEQEWQEIDNKAAGLRTLLQME"
     gene            complement(3380..3685)
                     /gene="elaB"
                     /locus_tag="ACHKBL_004322"
     CDS             complement(3380..3685)
                     /gene="elaB"
                     /locus_tag="ACHKBL_004322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein ElaB"
                     /protein_id="HFO5821099.1"
                     /translation="MSNQFGDTRIDDDLTLLSETLEEVLRSSGDPADQKYVELKARAE
                     KALDDVKKRVSQASDSYYYRAKQAVYRADDYVHEKPWQGIGVGAAVGLVLGLLLARR"
     gene            complement(3740..4201)
                     /gene="elaA"
                     /locus_tag="ACHKBL_004323"
     CDS             complement(3740..4201)
                     /gene="elaA"
                     /locus_tag="ACHKBL_004323"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO5821100.1"
                     /translation="MIDWQDLHHSDLSVSQLYALLQLRCAVFVVEQNCPYQDIDGDDL
                     EGENRHILGWHNGTLVAYARILKSDDELQPVVIGRVIVSEALRGEKIGQQLMSKALES
                     CTRRWPEKPIYLGAQAHLQNFYGQFGFIPVTDIYEEDGIPHIGMAREVIQA"
     gene            4266..5183
                     /gene="rbn"
                     /locus_tag="ACHKBL_004324"
     CDS             4266..5183
                     /gene="rbn"
                     /locus_tag="ACHKBL_004324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease BN"
                     /protein_id="HFO5821101.1"
                     /translation="MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQ
                     HQLLHTAFNPGKLDKIFISHLHGDHLFGLPGLLCSRSMSGIIQPLTIYGPHGIREFVE
                     TALRISGSWTDYPLEIVEIGAGEIFDDGLRKVTAYPLEHPLECYGYRIEEHDKPGALN
                     AQALKAAGVPPGPLFQALKAGKTITLEDGRQINGADYLAAPVPGKALAIFGDTGPCNA
                     ALDLAKGVDVMVHEATLDITMEAKANSRGHSSTRQAATLAREAGVGKLIITHVSSRYD
                     DKGCQHLLRECRSIFPVTELANDFTVFNV"
     gene            5371..>5481
                     /locus_tag="ACHKBL_004325"
     CDS             5371..>5481
                     /locus_tag="ACHKBL_004325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416772.1"
                     /note="ElaD; specific cysteine protease which targets
                     ubiquitin and ubiquitin-like proteins covalently bound to
                     target proteins; SseL in Salmonella is required for
                     macrophage killing and virulence, and it is secreted by
                     the salmonella pathogenicity island 2 type III secretion
                     system; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deubiquitinase"
                     /protein_id="HFO5821102.1"
                     /translation="MMVTVVSNYCQLSQTQLCQTFAEKFTVTEELLQSLKK"
BASE COUNT         1305 a         1506 c         1356 g         1314 t
ORIGIN      
        1 ccaccagcca gaagttaagg atgttgtaac taactaaggt tttacgcagt aagtcggtga
       61 catcatcaaa tccatcgacg ctaatcgccg ccgaaccacc gtgacctggg agatcaacat
      121 acaaccgtga gtagtcggca aacgcctcgc ccacttcttg ccattcatgg caatcgccgg
      181 aaaaaccgtg caaaaacacc agccagggta aacctggttt accgtgtttt gcctgcgcgt
      241 gcaggatcat aaatggctta cctgcgccag cagttgctgg agggtttgcg caccatcggt
      301 gtcgttaacc accatttcaa ttaccgtggt ggtcggcgtg cgccaggcgt cggcaagtgc
      361 cgtttcaagt tcctgccagt tttgcggacg atgatatttc agctcgaaca tcgcggcggc
      421 gtgctcaaaa tggacgtttt gcggcatcag atagaaacgc tcacgctcgt ttttcggcgt
      481 tggcaacagc gagaaaattt gcccgccgtt gttgttcacc acaattaata ccagcggcgc
      541 ggaaacctga cgtaataacg ccagcgcgtt gagatcgtaa agtgctgaga gatcgcccac
      601 aatcgccagc gtcggtttgc cactggcccg ctgaacgcca gcggcggtcg agagcagccc
      661 gtcgataccg ctggcaccac ggttgctgta caccgggtaa cctgccggaa gttgcgaaag
      721 cgcatcaatc agacgtacca ccaggctgtt accaacaaac aattgaccct gttcaggcag
      781 atagtcgctg atgcgatgcg ccagttgcgc ttcgccaaac gcatcgcgac gggcaataac
      841 cgcctgcatt gcctgttccg ccaggcgcgg aatttcaacg caccagggct ggcgtttttc
      901 tgccggatgc tgctccagcc agtcggcaat attggcaatt aagcgacgtc cacgatggtg
      961 tgccggatca agtcgccctt cgatgtcatc aacaatccag tactcttccg gttcacagct
     1021 tgcctgccat tgcaggagcc gtttgcccgt caggctgctt cccagttgca ccacaatttg
     1081 tgcctgttgc agttcgctgg tcgctttggc attgcctaac cagagatcgg cacacggcag
     1141 cggctgtccg gtttgcgaca gcacgtcgcc aatcagcggc cagccaagag tttgcgccca
     1201 cagtgcaact tttttgccct cttccgcact catgcgcccg gcaaccacca cgccgcgctt
     1261 ttgccgccag aagaaccagt cgcgctgttt ttcactttcc agacgaggcg cttcacgcag
     1321 ccacggtttg tcgtcctgcc accagtcgcc cagccgctgt tgccagctaa gcccggtgtc
     1381 gtccatttcg ccatacagcg gttcagcaaa cgggcagttg atatggactc cgcccgcatg
     1441 aagcgtaccg agagcatggt cgatggtaga aaccagccaa cgtgcaggaa tatcctgagt
     1501 cgggcgcggc agtgaaatac tgtgcgtggg gtgagaggcg aacattcccg gctgacgaat
     1561 cgcctgattc gcgccgcagt caattagctc cggcgggcga tcggcggtta acaggatcag
     1621 tttttctccg gttaacccag cttcaatcag tgccggatag agatttgcta ccgccgtgcc
     1681 ggaggtcaca atcaccgcca ccggctgctt gctgactttc gccagcccca gcgccaaatg
     1741 ccccagtcca cgctcatcga aatgggtgtg gtgaatgaat gcggaattct ccgccgccgc
     1801 taacgttaac ggtgtagaac gcgagccagg ggcgatacag atgtgtctga cgccgtgacg
     1861 cgttaatgct tccagaatga ccgccgccca gcgtcggtta aatgcgctta ctgacatgag
     1921 tttgtccggt atcaatattg cggctaagta taaggagcta caaaaataga ttattgatat
     1981 gaatcggtaa tgatgcgact cattactatt ccatttgtaa taaagtacgc agccctgccg
     2041 ctttgttatc gatttcctgc cactcttgct cggggtcaga accacggaca atgcccgcgc
     2101 cagcatatag ccgcacgaca ttgccactga tttttgctga gcgcagggaa acgcagaatt
     2161 cgctttgttg taaagagaga tagcccgcag aaccggcgta ccattctcgg gtgaacggtt
     2221 cgtgacgggc gataaactgt cgcgccagat cgcgcggtaa gccagcaact gctgccgttg
     2281 gctgcaactg atgtaaacag atcacatcat ccgctttgtt gagtgcagtc cagatacagc
     2341 ggcgaagatg ctgcacttta cgcagacgca gtacctgcgg cggtaaaaca tccaacgtct
     2401 gggtatcggc ctgcaatcgt tggcagatat cctccaccac caacatgttc tcgcgctggt
     2461 ttttatcatc cgccatcagc cactcaccta actgctgcgc ctgcttatca tcaggatgat
     2521 ttgctaccgt tcccgccagc gcttcagtac gcagcgcttt gtcacgccgc cgccataacc
     2581 gttccggtga agagccaaga aaagcaattt cgccatcaaa ggccatgtaa aaatggtagc
     2641 aattcagatt cagtcgacga ctggcagcca tcatcgccgc cgcattgacc ggacttgcga
     2701 aatgcaggtc agttgcccga gcaagcacca ctttgtcgag ctcaccttcg gcgatggttt
     2761 tcgttgccag ttcgattaat tgcgtccagc ccgttttgtc cggccagtgt tgttctcggg
     2821 tggtggttaa atgtaaccca ggcaagggtt taatactcac cagtgtggcg ataaattctt
     2881 ttgccttaat cgcatcatgc tgaagggagc tttcgctgaa tagcgtcagc cgcagcgtgg
     2941 ctttaccgcc acagcgtcgc cattcgaggc gtggtaaaag taaattgccc tgcgacgggt
     3001 caaacgcatt tagcccccag atgcgtaagt cggcgtgttc cgggtgctga cgaagaaacg
     3061 tttgtgcctg gtccaacgac gtaaaacggg taatcgcgcc caggacggca gcttcttcat
     3121 caccattacg ttgttgccag tagaattgcg ggtacgtttg ctgactggcc agccagctca
     3181 aggcatcaaa agcgtctttg agagggaaag gaatatcaat aacccgaatg ccgggtgtcg
     3241 ccggaatctc ttgcgacaaa tggcgcagta gattttccag cgccgtagta agtgattgca
     3301 cgcggacctc tccctgttaa aagcctcaca ttatacgggg tacgacaaaa aaaatgcagt
     3361 accccggtgt agggaggttt taacggcgtg ccagcaacag tcctagtacc agcccaacgg
     3421 ccgcgcccac accaattcct tgccagggtt tttcgtggac gtagtcatca gcacgataaa
     3481 cagcctgctt cgcccgatag taataactgt ctgaagcctg gctaacccgt tttttcacat
     3541 catccagcgc tttttctgca cgcgctttca gctcaacata tttctgatcg gcgggatcgc
     3601 cagaggagcg gagcacctct tccagtgttt cactaagcag cgtcaggtca tcatcaatac
     3661 gtgtatcacc aaactgatta gacattctcg ttctccattt gtgtaaaacc tgtcactaac
     3721 tatagacaag ggttattgat tacgcctgaa ttacctcgcg tgccatccca atgtgtggga
     3781 taccatcttc ttcataaata tcggtcacag ggatgaaacc aaattgccca taaaagtttt
     3841 gtaaatgcgc ctgcgccccc agataaatcg gcttctccgg ccagcggcga gtacacgatt
     3901 ccagcgcctt actcatcaat tgctgaccga ttttttcacc gcgtaaggct tcgctgacaa
     3961 tcacccgacc aatcacaacg ggttgcagtt cgtcgtcact tttcagaatc ctcgcatacg
     4021 ccaccagtgt cccgttatgc caaccgagaa tgtggcgatt ttctccctcc agatcgtcac
     4081 catcgatatc ctggtaagga cagttttgtt ccactacaaa taccgcacaa cgcagttgta
     4141 ataaggcata aagctgagaa acggataaat cagaatgatg cagatcttgc cagtcgatca
     4201 tggaaggctc ctttgtgctg tttgctttat actagccgga atattttcag cagcgtgatg
     4261 aactcatgga attaattttt ttaggtactt cagccggtgt gccaacccgt acgcgcaatg
     4321 tcacggcaat attacttaac ctgcaacatc cgacccagag cggtctttgg ttgtttgact
     4381 gcggtgaagg cacccagcat cagctactgc ataccgcctt taaccctgga aaactggaca
     4441 agatttttat cagtcacctt catggcgatc atctctttgg tttacccggc ttgctgtgca
     4501 gtcgttctat gtcaggcatt atccaaccct taacgattta tggtccgcac ggtatccgtg
     4561 aatttgtgga gaccgcgctg cggattagcg gctcatggac cgattatccg ctggaaattg
     4621 tcgaaattgg cgctggcgaa attttcgatg atggcctgcg caaagtgacc gcttatccgc
     4681 tggaacaccc actggaatgc tatggctatc gtattgaaga acatgataaa ccgggtgcat
     4741 taaatgctca ggcattaaaa gctgctggcg tgccgcctgg cccactgttt caggcattaa
     4801 aagcgggcaa aacaatcacg ctggaagacg gaaggcagat taacggcgca gattacttag
     4861 ccgctccagt gccaggtaaa gcgctcgcta ttttcggcga taccggcccc tgcaatgccg
     4921 cacttgacct ggctaaaggt gtcgatgtca tggtgcacga agcgacgctg gatataacca
     4981 tggaagccaa agccaatagt cgcggtcata gctctacacg ccaggctgcg acactggccc
     5041 gtgaggctgg agtcggtaag ctaatcatta cccacgtcag ctcgcgctat gatgacaaag
     5101 ggtgtcagca cctgttacgt gaatgcaggt caattttccc ggtgactgaa ctagcgaatg
     5161 atttcaccgt gtttaacgtt taactctgct tattattaaa aacagggcga aacttgccct
     5221 gttatcgcaa cccgcgctac atatatccac ccacacgggt atattgagcg atttttgata
     5281 tttattcatc tcctcacgca ctatattcag agcaataagt tgcctctttt tatcttcatt
     5341 cagcacaacc tttgcaacta aaaggagcct atgatggtta cagttgtcag caattattgt
     5401 caattatctc aaacccaact ctgtcagaca tttgcagaaa aatttactgt gaccgaggaa
     5461 ttactgcagt ctttaaaaaa a
//
LOCUS       DAZFLD010000251         5440 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.253, whole genome shotgun sequence.
ACCESSION   DAZFLD010000251 DAZFLD010000000
VERSION     DAZFLD010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5440
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.253"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            25..1359
                     /gene="garP"
                     /locus_tag="ACHKBL_004326"
     CDS             25..1359
                     /gene="garP"
                     /locus_tag="ACHKBL_004326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GarP"
                     /protein_id="HFO5821103.1"
                     /translation="MMLDTVDEKKKGVHTRYLILLIIFIVTAVNYADRATLSIAGTEV
                     AKELQLSAVSMGYIFSAFGWAYLLMQIPGGWLLDKFGSKKVYTYSLFFWSLFTFLQGF
                     VDMFPLAWAGISMFFMRFMLGFSEAPSFPANARIVAAWFPTKERGTASAIFNSAQYFS
                     LALFSPLLGWLTFAWGWEHVFTVMGVIGFVLTALWIKLIHNPTDHPRMSAEELKFISE
                     NGAVVDMDHKKPGSAAASGPKLHYIKQLLSNRMMLGVFFGQYFINTITWFFLTWFPIY
                     LVQEKGMSILKVGLVASIPALCGFAGGVLGGVFSDYLIKRGLSLTLARKLPIVLGMLL
                     ASTIILCNYTNNTTLVVMLMALAFFGKGFGALGWPVISDTAPKEIVGLCGGVFNVFGN
                     VASIVTPLVIGYLVSELHSFNAALVFVGCSALMAMVCYLFVVGDIKRMELQK"
     gene            1375..2146
                     /gene="garL"
                     /locus_tag="ACHKBL_004327"
                     /pseudo
     CDS             1375..2146
                     /gene="garL"
                     /locus_tag="ACHKBL_004327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312031.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008672 - 2-dehydro-3-deoxyglucarate
                     aldolase activity [Evidence IEA];
                     GO_process: GO:0019394 - glucarate catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyglucarate aldolase"
     gene            2176..3066
                     /gene="garR"
                     /locus_tag="ACHKBL_004328"
     CDS             2176..3066
                     /gene="garR"
                     /locus_tag="ACHKBL_004328"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008679 - 2-hydroxy-3-oxopropionate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0046487 - glyoxylate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HFO5821104.1"
                     /translation="MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAA
                     GAETASTAKAIAEQCDVIITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLA
                     SREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
                     HTGEIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLD
                     AKAPMVMDRNFKPGFRIDLHIKDLANALDTSHGVGAQLPLTAAVMEMMQALRADGLGT
                     ADHSALACYYEKLAKVEVTR"
     gene            3163..4308
                     /gene="garK"
                     /locus_tag="ACHKBL_004329"
     CDS             3163..4308
                     /gene="garK"
                     /locus_tag="ACHKBL_004329"
                     /EC_number="2.7.1.165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417593.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0031388 - organic acid phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 2-kinase"
                     /protein_id="HFO5821105.1"
                     /translation="MKIVIAPDSYKESLSASEVAQAIEKGFREIFPDAQYVSVPVADG
                     GEGTVEAMIAATQGAERHAWVTGPLGEKVNASWGISGDGKTAFIEMAAASGLELVPAE
                     KRDPLVTTSRGTGELILQALESGATNIIIGIGGSATNDGGAGMVQALGAKLCDANGNE
                     IGFGGGSLNTLNDIDISGLDPRLKDCVIRVACDVTNPLVGDSGASRIFGPQKGASEAM
                     IVELDNNLSHYADVIKKALHVDVKDVPGAGAAGGMGAALMAFLGAELKSGIEIVTTAL
                     NLEEHIHDCTLVITGEGRIDSQSIHGKVPIGVANVAKKYHKPVIGIAGSLTNDVGVVH
                     QHGIDAVFSVLTSIGTLDEAFRGAYDNICRASRNIAATLAIGMRNAG"
     gene            4341..4717
                     /gene="rnpB"
                     /locus_tag="ACHKBL_004330"
     ncRNA           4341..4717
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACHKBL_004330"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(4926..5027)
                     /locus_tag="ACHKBL_004331"
                     /pseudo
     CDS             complement(4926..5027)
                     /locus_tag="ACHKBL_004331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417590.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5283..>5440)
                     /gene="tdcR"
                     /locus_tag="ACHKBL_004332"
     CDS             complement(5283..>5440)
                     /gene="tdcR"
                     /locus_tag="ACHKBL_004332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional activator TdcR"
                     /protein_id="HFO5821106.1"
                     /translation="HIFSRYLLENKHLFYQYFKISNTGIDHLEQLINVNFFSSDRTSF
                     CECNRFP"
BASE COUNT         1303 a         1265 c         1516 g         1356 t
ORIGIN      
        1 gcataagtac ccgaggtaaa taaaatgatg ctggacaccg ttgacgaaaa aaagaaaggc
       61 gtgcataccc gctatttaat attactgatt atttttattg ttaccgccgt taactacgcc
      121 gatcgtgcaa cgctgtctat tgctggtacc gaagtggcaa aagagttgca gttaagtgcg
      181 gtttcgatgg gttacatctt ctctgctttt ggctgggcct acttgctgat gcaaatcccc
      241 ggcggctggc tgcttgataa gtttggttcg aaaaaagttt acacctacag cctctttttc
      301 tggtcgctat tcaccttcct gcaaggcttt gttgatatgt tcccgctggc ctgggcaggg
      361 atctccatgt tctttatgcg ctttatgctg gggttctcgg aagcgccatc attcccggcg
      421 aacgcccgta ttgtcgccgc ctggttcccg acgaaagaac gtggtactgc ctccgccatc
      481 tttaactcgg cgcaatattt ctcgctggcg ctcttttcgc cgctgcttgg ctggctgact
      541 ttcgcgtggg gctgggagca cgtctttacc gttatggggg taattggttt tgtgctgacg
      601 gcgctgtgga tcaagttgat tcataacccg acagatcacc cacgtatgtc tgcggaagag
      661 ctgaagttta tctcggaaaa tggcgcggtg gtcgatatgg accacaaaaa gccgggcagt
      721 gcggcagcaa gcggacccaa actgcattac atcaagcaat tgctctctaa ccgcatgatg
      781 ctgggcgtat ttttcggaca atattttatc aacaccatca cctggttctt cctcacctgg
      841 ttcccaattt atctggtgca ggaaaaaggc atgtcgattc tgaaagtggg tctggtcgcc
      901 tcgattccgg cactgtgtgg ctttgcgggc ggcgtgctgg gaggtgtctt ctcggattat
      961 ctgatcaaac gcggtttgtc cctgaccctg gcacgtaagc taccaattgt gctgggaatg
     1021 ttgctggctt ccaccatcat cttatgtaac tacaccaaca acaccacgct ggtggtcatg
     1081 ctgatggcgc tggctttctt tggtaaagga tttggtgcgc tgggctggcc agtgatttct
     1141 gacaccgcgc cgaaagagat tgttggcctc tgcggcggcg tctttaacgt ctttggcaat
     1201 gttgcctcca ttgtcactcc actggtgatt ggctacctgg taagtgaact gcactccttc
     1261 aatgcagcac tggtttttgt gggatgttca gcgctgatgg cgatggtctg ctacctcttc
     1321 gtagtcggcg acattaaacg tatggaattg cagaaataag caaaggtata agcgatgaat
     1381 aacgatgttt tcccgaataa attcaaagcc gcactggctg cgaaacaggt accaaattgg
     1441 ttgctggtca gctctctcta acccgattag cactgaagtt cttggtttgg ctgggtttga
     1501 ctggctggtg ctggatggtg aacatgcgcc aaacgatatt tccacgttta ttccgcagtt
     1561 aatggccctg aaaggcagcg ccagcgcgcc agtagtgcga gtgccgacca acgagccggt
     1621 aattattaag cgtcttctgg atatcggttt ctataacttc ctgattcctt ttgtagaaac
     1681 aaaagaggaa gcagagcagg cggtggcatc aactcgttac ccaccggaag gcattcgcgg
     1741 cgtctccgtt tctcaccgcg ccaatatgtt tggcaccgtg gcggattatt tcgctcagtc
     1801 gaacaagaac atcactattc tggtccagat agaaagtcag cagggcgtag ataacgtcga
     1861 tgctattgcc gctaccgaag gtgtagacgg catcttcgtc ggccccagcg atctggctgc
     1921 ggcattaggc catctcggca atgcctcgca cccggatgta caaaaagcaa ttcagcacat
     1981 ttttaaccgt gccagcgcgc acggcaaacc cagcggcatc ctcgcaccag tcgaggctga
     2041 tgcgcgtcgt tatctggaat ggggcgcgac gtttgtggcc gtcggcagcg atctcggcgt
     2101 cttccgctct gccactcaga aactggctga tacctttaaa aaataaccac caccgcaata
     2161 agagagatga ttgatatgac tatgaaagtt ggttttattg gccttgggat tatgggtaaa
     2221 ccaatgagta aaaacctttt gaaagcaggt tactcgctgg tggttgctga ccgtaaccca
     2281 gaagctatcg ctgacgtgat tgctgcaggt gcagaaacag cctctacggc taaagcgatc
     2341 gctgaacagt gcgacgtaat cataaccatg ctgccaaact cccctcatgt gaaagaggtg
     2401 gcgctgggtg agaatggcat tattgaaggc gcgaagccag gtacggtatt gatcgatatg
     2461 agttctatcg caccgctggc aagccgtgaa atcagcgaag cgctgaaagc gaaaggcatt
     2521 gatatgctgg atgctccggt gagcggcggt gaaccgaaag ccatcgacgg tacgctgtca
     2581 gtgatggtgg gcggcgacaa ggctattttc gacaaatact atgatttgat gaaagcgatg
     2641 gcgggttccg tggtgcatac cggggaaatc ggtgcaggta acgtcaccaa actggcaaat
     2701 caggtcattg tggcgctgaa tattgctgcg atgtcagaag cgttaacgtt ggcaaccaaa
     2761 gcgggcgtta acccggatct ggtgtatcag gcaattcgcg gtgggctggc gggcagtacc
     2821 gtgctggatg ccaaagcgcc gatggtgatg gaccgcaact tcaaaccggg cttccgtatt
     2881 gatctgcata ttaaggatct ggcgaatgcg ctggatactt ctcacggcgt cggcgcgcaa
     2941 ctgccgctca cagctgcggt tatggagatg atgcaggcac tgcgtgcaga tggtttagga
     3001 acggcggatc atagcgccct ggcgtgctac tacgaaaaac tggcgaaagt cgaagttact
     3061 cgctaatgac gtcgtgcccg gatggcgtat tgcaatccgg gcctggaatc caggccgaat
     3121 aagagaaacg ccctccggcg tcatgtggta acaggcatag gtatgaaaat cgtaatcgcc
     3181 ccagactctt ataaagaaag tttatctgcc agcgaggttg cgcaggcgat agaaaaagga
     3241 tttcgggaaa tttttcctga tgcacagtat gtttctgttc cggttgccga cggtggtgaa
     3301 ggaacggtgg aagcgatgat tgcagccacc cagggggctg aacgtcacgc ctgggttaca
     3361 gggccgctgg gcgagaaagt gaatgccagt tgggggatct ccggcgatgg caaaaccgcg
     3421 tttatcgaaa tggcggcggc cagtgggctg gaactggtac ctgcggaaaa acgcgatcca
     3481 ctcgtgacca cttcacgcgg tactggtgag ttaatcctgc aggcgctgga gagcggtgcg
     3541 acaaacatta ttatcggcat tggcggcagc gccaccaacg acggcggcgc aggcatggta
     3601 caggcgctgg gggcgaaatt atgcgatgcc aacggcaatg aaattggttt tggcggcggt
     3661 agtcttaata ctctgaatga tattgatatt tccggtctcg atccgcgctt aaaagattgt
     3721 gtcatccgcg tcgcttgtga tgtcaccaac ccgttagtcg gcgacagtgg tgcttcgcgc
     3781 atctttggcc cacaaaaggg agccagtgag gcgatgattg tcgagctgga caataacctc
     3841 tctcactatg ccgatgttat taaaaaagcg ctgcatgttg atgtgaaaga tgtccccggc
     3901 gcaggagctg cgggcggtat gggcgcggcg ctgatggcgt ttcttggtgc agaactgaaa
     3961 agtggtattg aaatcgtcac tacggcgctg aatctggagg aacatattca cgattgtacg
     4021 ctggtgatca ctggtgaagg gcgtattgat agccagagta ttcacggcaa ggtaccgatt
     4081 ggtgtcgcaa acgtggcgaa gaagtaccat aaaccggtga ttggcattgc gggtagcctg
     4141 accaatgatg ttggcgttgt acatcagcat ggcattgatg cggtcttcag cgtattgacc
     4201 agcattggta cgttggacga agcattccgc ggggcttatg acaatatctg ccgtgcttca
     4261 cgtaatatcg ccgcgacact ggcgattgga atgcgcaacg cggggtgaca agggcgcgca
     4321 aaccctctat actgcgcgcc gaagctgacc agacagtcgc cgcttcgtcg tcgtcctctt
     4381 cgggggagac gggcggaggg gaggaaagtc cgggctccat agggcagggt gccaggtaac
     4441 gcctgggggg gaaacccacg accagtgcaa cagagagcaa accgccgatg gcccgcgtaa
     4501 gcgggatcag gtaagggtga aagggtgcgg taagagcgca ccgcgcggct ggtaacagtc
     4561 cgtggcacgg taaactccac ccggagcaag gccaaatagg ggttcataag gtacggcccg
     4621 tactgaaccc gggtaggctg cttgagccag tgagcgattg ctggcctaga tgaatgactg
     4681 tccacgacag aacccggctt atcggtcagt ttcacctgat ttacgtaaaa acccgcttcg
     4741 gcgggttttt gcttttggtg cccagggtaa gatgaatatc tacggtatta ataatagact
     4801 ttgtaagaag ttattaagtc tatattgcat ttaaaaggtc gtaattatac atatatttct
     4861 ctcattaatt gtaagttcgc aggtattgtc cgatcgttga atcatttgga cgttataacc
     4921 taaattttta ccgtccaata atcaatattt ctgttgtgat tgttaaccac tgcgtgcatt
     4981 ggcgggattg aagggtgaaa aatatgtgca attgggaagc ctttcattga tgcctgcctt
     5041 gtcatcatga caaacaataa tagatgctta ttttcctcgc agggttcttt cgcactatca
     5101 aaaaatgctc atttacccgg cattacagtc aacgaagcaa cattctatgg cctacgctga
     5161 tgtaaagcac agtgagaatc ccccttgttg tcttcgtctg accttatcgc ggcgaccagc
     5221 aattattcgt tttgtgatcc tcaacacaaa atgctattcg tcgcctatga ggggagagga
     5281 atttatggaa aacgattgca ttcacaaaat gacgtcctgt cagaagagaa aaagttgacg
     5341 ttaatcaatt gttcgagatg atcgatacct gtattcgaaa ttttaaaata ttgataaaat
     5401 aaatgtttgt tctccaacaa atagcgtgaa aaaatgtgac
//
LOCUS       DAZFLD010000252         5425 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.254, whole genome shotgun sequence.
ACCESSION   DAZFLD010000252 DAZFLD010000000
VERSION     DAZFLD010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5425
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.254"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..69
                     /locus_tag="ACHKBL_004333"
     CDS             <1..69
                     /locus_tag="ACHKBL_004333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5821107.1"
                     /translation="EETLLQQPEERFAWLRSKKDRF"
     gene            141..1499
                     /gene="mltD"
                     /locus_tag="ACHKBL_004334"
     CDS             141..1499
                     /gene="mltD"
                     /locus_tag="ACHKBL_004334"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase D"
                     /protein_id="HFO5821108.1"
                     /translation="MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQA
                     RWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYM
                     YWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
                     DARRDVVASTTAALNMMQRLNKMFDGDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDF
                     WSLPLPQETKQYVPKMLALSDILKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVAD
                     MAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRESLASGEIAAVQSTLVAD
                     NTPLNSRVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGAGSSAQR
                     LANNSDSITYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNNMP
                     DS"
     gene            complement(1546..2316)
                     /locus_tag="ACHKBL_004335"
     CDS             complement(1546..2316)
                     /locus_tag="ACHKBL_004335"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFO5821109.1"
                     /translation="MTTQSHHDHVEKQFSSQASEYLTSTVHASGRDLQRLAVRLADYP
                     DASVLDMGCGAGHASFVAAQNVSAVVAYDLSAQMLDVVAQAAEARHLKNIATRQGYAE
                     SLPFADNAFDIVISRYSAHHWHDVGAALREVNRILKPGGRLIVMDVMSPGHPVRDVWL
                     QTVEALRDTSHVRNYASGEWLTLINEANLIVDNLITDKLPLEFSSWVARMRTPEALVD
                     AIRIYQQSASTEVKTYFALQNDGSFTSDIIMVEAHKAA"
     gene            complement(2393..3193)
                     /gene="yafD"
                     /locus_tag="ACHKBL_004336"
     CDS             complement(2393..3193)
                     /gene="yafD"
                     /locus_tag="ACHKBL_004336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFO5821110.1"
                     /translation="MRKNTYAMRYVAGQPAERILPPGSFASIGQALPPGEPLSTEERI
                     RILVWNIYKQQRAEWLSVLKNYGKDAHLVLLQEAQTTPELVQFATANYLAADQVPAFV
                     LPQHPSGVMTLSAAHPVYCCPLREREPILRLAKSALVTVYPLPDTRLLMVVNIHAVNF
                     SLGVDVYSKQLLPIGDQIAHHSGPVIMAGDFNAWSRRRMNALYRFAREMSLRQVRFTD
                     DQRRRAFGRPLDFVFYRGLNVSEASVLVTRASDHNPLLVEFSPGKPDK"
     gene            3434..4348
                     /gene="yafC"
                     /locus_tag="ACHKBL_004337"
     CDS             3434..4348
                     /gene="yafC"
                     /locus_tag="ACHKBL_004337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YafC"
                     /protein_id="HFO5821111.1"
                     /translation="MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMK
                     LGVSLLNRTTRQLSLTEEGERYFRRVQSILQEMAAAESEIMETRNTPRGLLRIDAATP
                     VVLHFLMPLIKPFRERYPEVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRARPLF
                     NSYRKIIASPDYISRYGKPETIDDLKQHVCLGFTEPASLNTWPIACSDGQLHEVKYGL
                     SSNSGETLKQLCLSGNGIACLSDYMIDREIARGELVELMADKVLPVEMPFSAVYYSDR
                     AVSTRIRAFIDFLSEHVKTAPGGAVREA"
     gene            complement(4345..5148)
                     /gene="dkgB"
                     /locus_tag="ACHKBL_004338"
     CDS             complement(4345..5148)
                     /gene="dkgB"
                     /locus_tag="ACHKBL_004338"
                     /EC_number="1.1.1.346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgB"
                     /protein_id="HFO5821112.1"
                     /translation="MAIPAFGLGTFRLKDDVVISSVKTALELGYRAIDTAQIYDNEAA
                     VGQAIAESGVPRHELYITTKIWIENLSKDKLIPSLKESLQKLRTDYVDLTLIHWPSPN
                     DEVSVEEFMQALLEAKKQGLTREIGISNFTIPLMEKAIAAVGAENIATNQIELSPYLQ
                     NRKVVAWAKQHGIHITSYMTLAYGKALKDEVIARIAAKHNATPAQVILAWAMGEGYSV
                     IPSSTKRKNLESNLKAQNLQLDAEDKKAIAALDCNDRLVSPEGLAPEWD"
     gene            complement(5310..5386)
                     /locus_tag="ACHKBL_004339"
     tRNA            complement(5310..5386)
                     /locus_tag="ACHKBL_004339"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5350..5352),aa:Asp,seq:gtc)
BASE COUNT         1420 a         1321 c         1349 g         1335 t
ORIGIN      
        1 gaagaaacat tattgcaaca acctgaagaa cgttttgcat ggttaaggtc aaagaaagat
       61 aggttctgat aaaactttct tgtcatcggc tccgttcgcc gttatgatcg gtcgtctttt
      121 aagcaactat tgacacacac atgaaggcaa aagcgatatt actcgcctct gtcctgctcg
      181 tgggttgcca gagtaccggc aacgttcaac agcacgcaca gagcctttct gcagctggtc
      241 aaggggaagc agcaaagttt acaagtcagg cacgatggat ggacgatggg acgtctatcg
      301 cgccagatgg tgacttgtgg gctttcattg gcgacgagct aaagatggga attccggaaa
      361 atgaccggat tcgcgaacag aaacagaaat atttacgcaa taagagctat ctccacgatg
      421 taactttacg ggcagagccg tatatgtact ggatagccgg gcaagttaaa aaacgtaaca
      481 tgcctatgga actggtacta ctacccatag tggagagcgc ttttgatcct cacgcaacgt
      541 ctggcgccaa tgccgcaggc atctggcaga tcattccgag cacggggcgc aattatggtt
      601 tgaaacagac ccgcaattat gacgcgcgtc gcgatgttgt tgcttcaaca actgccgcgc
      661 tgaacatgat gcagcgtctg aacaaaatgt ttgacggcga ctggcttctg accgtagctg
      721 cttataacag cggcgaaggt cgagtcatga aggcaattaa aacgaacaaa gcgcgtggga
      781 aatccacgga cttctggtcg ttaccgttgc cgcaggaaac gaagcagtac gtgcctaaga
      841 tgctggcatt gagtgatatt ctcaaaaaca gcaagcgtta tggcgtacgt ctgccaacga
      901 ccgatgaaag ccgtgctctg gcacgtgtgc acctgagcag cccggttgaa atggcgaagg
      961 ttgcagatat ggcggggatt tccgtcagca agctgaagac attcaacgca ggcgtgaaag
     1021 gctccacgct gggcgcaagt ggtccgcagt acgtgatggt gccaaagaag catgcagatc
     1081 aactgcgtga atctctggct tcaggcgaaa ttgctgctgt acagtcgacg ctggttgccg
     1141 acaatacgcc gcttaacagc cgtgtttaca ccgtacgctc tggcgacacg ctttcaagta
     1201 tcgcttcacg tctcggcgta agcaccaaag atttgcagca gtggaacaaa ctgcgcggat
     1261 ctaagctgaa gccaggccaa agtttgacga ttggtgcagg cagtagcgca cagcgactgg
     1321 caaacaacag cgatagcatt acgtatcgtg tgcgcaaagg cgattcgctt tcaagcattg
     1381 ctaaacgcca cggcgtgaac atcaaagatg taatgcgctg gaacagcgac actgcgaatc
     1441 tgcaaccagg cgataagctg acgttgtttg tgaaaaacaa caacatgcca gattcctgac
     1501 aaaccagata ataaaaggca ccgattcccc cggtgccttt tttatttatg ccgctttatg
     1561 tgcttctacc atgatgatat cactggtgaa agagccatca ttctgcaagg caaaatacgt
     1621 tttcacctct gtcgatgcgc tctgttggta aatgcgaata gcgtctacta acgcttctgg
     1681 cgtacgcatt ctcgcgaccc atgaagaaaa ttccagcggt aacttatctg taattaaatt
     1741 atcaactatc agattggctt cattgattaa cgtcaaccac tcaccgctgg cgtagtttcg
     1801 tacgtgagag gtatcgcgta atgcttctac cgtctgtaac cagacgtcgc gcactgggtg
     1861 acccggagac attacgtcca tcacaatcag cctaccgcca ggtttcaata tcctattcac
     1921 ttctcgcagt gctgcaccaa catcatgcca gtgatgggca gaataacggc tgataacaat
     1981 atcaaatgcg ttatcggcaa atggcagact ttcggcatat ccctggcggg tggcgatatt
     2041 tttcaggtgc cgggcttcgg cagcttgtgc cacgacatcc agcatttggg cagataagtc
     2101 atacgccacc accgcgctca cgttctgcgc agcgacaaag ctggcatgcc ctgctccgca
     2161 gcccatatca agtacacttg catcaggata atcagccaga cgcaccgcca ggcgctgtaa
     2221 atctcgcccg gatgcatgta cggtactggt taaatactca ctggcctgcg agctaaactg
     2281 cttttctacg tggtcatggt gggattgtgt tgtcattgcg ctgtcctttt attgtttgat
     2341 taaaaagggc agcgccaaac gttggtctgc cctgtggcag acctgacata ccttatttat
     2401 caggcttgcc gggactgaat tcaacgagta gcggattgtg atcggaagcg cgcgtaacca
     2461 gtacagaagc ttcgctgacg ttcagaccac ggtagaaaac aaaatcgagc gggcggccaa
     2521 acgcccgacg gcgctgatca tcggtaaaac gcacctggcg cagcgacatt tcccgcgcaa
     2581 agcgatataa cgcgttcatc cttctacggc tccaggcatt gaaatctccc gccataatga
     2641 ccgggccgct gtggtgagct atctgatcgc caataggaag taactgctta ctatagacat
     2701 ccacgcccaa actgaaattg acggcgtgta tattaaccac catcaacagg cgggtgtcag
     2761 gcaatggata gaccgtcacc agtgccgact tcgccagacg caaaatgggt tctcgttcac
     2821 gtaacgggca gcaatacact ggatgcgccg ccgaaagggt cattacaccg gaaggatgtt
     2881 gtggcagcac gaaagcgggt acctgatcgg cggcaagata gttagcggtc gcaaactgca
     2941 ctaactctgg cgttgtctgc gcttcctgca ataacaccag atgtgcatct ttgccgtagt
     3001 tctttaatac cgacaaccat tcagcgcgtt gctgtttgta tatgttccac accaggatcc
     3061 gaatacgctc ttcggtactt aacggttctc caggtggtaa tgcctggccg atgctcgcaa
     3121 aagaccccgg cggtaagatc ctttccgcag gttgtccagc aacatagcgc atggcatagg
     3181 tgttttttcg cacttttgac tttgaaacct ctattacgtc aacccttccg cactcgggag
     3241 ggttctactg ttatagggat tttagctcac tctttcaacg agcaattgta tattgctatg
     3301 taagcaagtg cttacaccgg tcagaaattt gctcattatt gagaacatta aatcggatga
     3361 catatctcgc atcctgatgg tttaatgaca attaaattca aaatatctct tctattttgc
     3421 aacaggagca aaaatgaaag ccacgtcgga agaactcgcc atttttgttt cagtcgtcga
     3481 aagcggcagc tttagccggg cagcggaaca attagggcaa gcaaactcag cggtaagccg
     3541 ggcggtgaaa aagctggaga tgaaacttgg cgttagcctg cttaatcgga ccacgcggca
     3601 acttagcctg acggaagaag gcgagcgtta ttttcgtcgc gtacagtcaa ttttgcagga
     3661 gatggcagcg gcagaatcag aaattatgga gacgcgtaat acaccgcgtg gactgttacg
     3721 gatcgatgcc gcaactccag tggtgctgca ctttctgatg ccgttgatta agccttttcg
     3781 tgaacgctat ccggaagtca ctttatcgct agtctcctcc gaaacgatta ttaatttgat
     3841 cgaaagaaaa gtggatgtcg cgatacgcgc tggtacgtta acggattcca gcttacgtgc
     3901 caggccgtta tttaacagtt atcgaaaaat tatcgcctcc cccgattata tttcccgcta
     3961 cgggaagcca gaaacgatcg acgatttaaa gcaacatgtt tgcctgggat tcactgaacc
     4021 cgcttccctc aatacctggc cgatagcctg tagtgatgga caattacatg aggtgaagta
     4081 cggtttgtca tccaatagtg gggaaacact gaaacagctt tgcctgagtg gcaacgggat
     4141 tgcgtgtttg tccgactaca tgatcgacag agaaatcgct cgcggagaat tggtggagtt
     4201 aatggcagat aaagtattgc cagtggaaat gccattcagt gccgtatatt acagcgaccg
     4261 tgcggtaagt acgcgcatcc gggcttttat cgatttcctt agcgagcatg taaaaacagc
     4321 tcccggagga gctgtcagag aggcttaatc ccattcagga gccagacctt ccgggctaac
     4381 caggcggtcg ttgcaatcca gtgcggcgat cgctttttta tcttcggcat caagctgtaa
     4441 attttgtgcc ttaagattac tttccaggtt tttacgttta gtagaagaag gaattacgga
     4501 gtaaccttcc cccatagccc acgccagaat cacttgtgcc ggagtcgcat tgtgtttagc
     4561 tgcgatacga gcaataacct catctttcag ggccttacca tacgccagcg tcatatagga
     4621 agtaatatgg atgccgtgct gtttagccca ggcgaccact ttacggtttt gcagataagg
     4681 agagagttca atctggttag tagcgatgtt ttcagcgcca acagcagcaa tcgccttttc
     4741 catcaatggg atcgtgaagt tggaaatacc gatctcacgc gtcagccctt gttttttggc
     4801 ttccagcagc gcctgcataa actcttcaac agagacttca tcgtttggtg acggccagtg
     4861 gattagcgtc agatcaacat aatcggtacg caatttttgc aggctctctt tcagactcgg
     4921 gatcaatttg tctttgctga gattttcaat ccagatttta gtggtgatgt agagttcatg
     4981 acgtggcacg ccactttctg caatcgcctg acctactgcg gcttcgttat catagatttg
     5041 tgcggtatca attgcgcgat aaccaagttc aagcgccgtt ttcacagatg aaataacaac
     5101 gtcgtctttc agacggaaag tacctaaacc aaatgcaggg atagccataa tatgcctctt
     5161 ttaatgctat tttcgttaac tgaggtgatt atgggcttca gactgttgcg gaaaaagagc
     5221 gtaaaacgca gaggattttt gcgattctgg caataataga aatacagacg aaaaaaacct
     5281 cgagcgttaa ctcgaggctt cttaataagt ggcggaacgg acgggactcg aacccgcgac
     5341 cccctgcgtg acaggcaggt attctaaccg actgaactac cgctccaccg aattctttta
     5401 caaccaccgg ttttatgacc ggctt
//
LOCUS       DAZFLD010000253         5406 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.255, whole genome shotgun sequence.
ACCESSION   DAZFLD010000253 DAZFLD010000000
VERSION     DAZFLD010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5406
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.255"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(103..1386)
                     /gene="aroA"
                     /locus_tag="ACHKBL_004340"
     CDS             complement(103..1386)
                     /gene="aroA"
                     /locus_tag="ACHKBL_004340"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFO5821113.1"
                     /translation="MESLTLQPIARVDGTINLPGSKSVSNRALLLAALAHGKTVLTNL
                     LDSDDVRHMLNALTALGVSYTLSADRTRCEIIGNGGPLHAEGALELFLGNAGTAMRPL
                     AAALCLGSNDIVLTGEPRMKERPIGHLVDALRLGGAKITYLEQDNYPPLRLQGGFTGG
                     NVDVDGSVSSQFLTALLMTAPLAPEDTVIRIKGDLVSKPYIDITLNMMKTFGVEIENQ
                     HYQQFVVKGGQSYQSPGTYLVEGDASSASYFLAAAAIKGGTVKVTGIGRNSMQGDIRF
                     ADVLEKMGATICWGDDYISCTRGELNAIDMDMNHIPDAAMTIATAALFAKGTTTLRNI
                     YNWRVKETDRLFAMATELRKVGAEVEEGHDYIRITPPEKLNFAEIATYNDHRIAMCFS
                     LVALSDTPVTILDPKCTAKTFPNYFEQLARISQAA"
     gene            complement(1457..2545)
                     /gene="serC"
                     /locus_tag="ACHKBL_004341"
     CDS             complement(1457..2545)
                     /gene="serC"
                     /locus_tag="ACHKBL_004341"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HFO5821114.1"
                     /translation="MAQIFNFSSGPAMLPAEVLKQAQQELRDWNGLGTSVMEVSHRGK
                     EFIQVAEEAEKDFRDLLNVPSNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYW
                     AASAIKEAKKYCTPNVFDAKVTVDGLRAVKPMREWQLSDNAAYMHYCPNETIDGIAID
                     ETPDFGKDVVVAADFSSTILSRPIDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKA
                     NIACPSILDYSILNDNDSMFNTPPTFAWYLSGLVFKWLKANGGVAAMDKINQQKAELL
                     YGVIDNSNFYRNDVAKANRSRMNVPFQLADSALDKLFLEESFAAGLHALKGHRVVGGM
                     RASIYNAMPLEGVKALTDFMVEFERRHG"
     gene            complement(2744..3436)
                     /locus_tag="ACHKBL_004342"
     CDS             complement(2744..3436)
                     /locus_tag="ACHKBL_004342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HFO5821115.1"
                     /translation="MKAFDLHRMAFDKVPFDFLGEVALRSLYTFVLVFLFLKMTGRRG
                     VRQMSLFEVLIILTLGSAAGDVAFYDDVPMVPVLIVFIILALLYRLVMWLMAHSEKLE
                     DLLEGKPVVIIEDGELAWSKLNNSNMTEFEFFMELRLRGVEQLGQVRLAILETNGQIS
                     VYFFEDDKVKPGLLILPSDCTQRYKVVPESVDYACIRCSEIIHMNAGEKQLCPRCANP
                     EWTKASRAKRVT"
     gene            3566..5326
                     /gene="ycaO"
                     /locus_tag="ACHKBL_004343"
     CDS             3566..5326
                     /gene="ycaO"
                     /locus_tag="ACHKBL_004343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     accessory factor YcaO"
                     /protein_id="HFO5821116.1"
                     /translation="MTQTFIPGKDAALEDSIARFQQKLSDLGFQIEEASWLNPVPNVW
                     SVHIRDKECALCFTNGKGATKKAALASALGEYFERLSTNYFFADFWLGETIANGPFVH
                     YPNEKWFPLSENDDVPEGLLDDRLRAFYDPENELAGSMLIDLQSGNEDRGICGLPFTR
                     QSDNQTVYIPMNIIGNLYVSNGMSAGNTRNEARVQGLSEVFERYVKNRIIAESISLPE
                     IPADVLARYPAVVEAIETLEAEGFPIFAYDGSLGGQYPVICVVLFNPANGTCFASFGA
                     HPDFGVALERTVTELLQGRGLKDLDVFTPPTFDDEEVAEHTNLETHFIDSSGLISWDL
                     FKQDADYPFVDWNFSGTTEEEFATLMAIFNKEDKEVYIADYEHLGVYACRIIVPGMSD
                     IYPAEDLWLANNSMGSHLRETILSLPGSEWEKEDYLNLIEQLDEEGFDDFTRVRELLG
                     LATGSDNGWYTLRIGELKAMLALAGGDLEQALVWTEWTMEFNSSVFSPERANYYRCLQ
                     TLLLLAQEEDRQPLQYLNAFVRMYGADAVEAASAAMSGEAAFYGLQPVDSDLHAFAAH
                     QSLLKAYEKLQRAKAAFWAK"
BASE COUNT         1380 a         1382 c         1292 g         1352 t
ORIGIN      
        1 ctaccattaa tcaaaatgac tcacaaggtc cgaaaaaaaa cgccggcaat gtgccgacgt
       61 tttgataaag aaagatttgg ctatttattg cacgttgttc attcaggctg cctggctaat
      121 ccgcgccagc tgctcgaaat aattcggaaa tgttttggcc gtgcatttgg gatcaagaat
      181 cgtcactggt gtatctgaca acgccaccag cgagaaacac atcgctatcc ggtgatcatt
      241 gtatgtcgcg atctcggcaa agttcagctt ttccggtgga gtgatacgaa tgtaatcgtg
      301 cccctcttct acttccgcac caactttacg cagttctgtt gccatcgcaa acaggcgatc
      361 ggtctcttta acacgccagt tatagatatt gcgcagcgtg gtggtgcctt ttgcaaataa
      421 cgccgccgtg gcaatggtca tcgccgcatc gggaatatgg ttcatatcca tatcaatagc
      481 gttcagttca ccccgcgtgc aggaaatata atcatcgccc cagcaaatgg tcgcgcccat
      541 tttttccagc acatcagcaa agcgaatatc accctgcata ctgttgcgtc caatgccggt
      601 cacttttaca gtgccgcctt tgattgctgc tgctgccagg aagtaagaag ccgaagatgc
      661 atcgccttcg accaaataag tacccggaga ctgataagac tgcccgcctt ttacgacaaa
      721 ttgttgatag tgctgatttt caatttcaac accaaacgtc ttcatcatat tgagtgtgat
      781 gtcgatataa ggtttagaaa ccagatcgcc tttaatacga atcaccgtat cttccggtgc
      841 aagaggcgca gtcattaata gtgcggtgag gaactggctg gaaacggagc catcaacgtc
      901 aacgttgccg ccagtaaagc cgccctgtaa acgcaatggc ggataattgt cttgttccag
      961 gtaagtgatc ttcgccccgc ccaggcgcag cgcatccacc agatgaccaa tcgggcgctc
     1021 tttcatacgc ggctcaccag tcagcacaat atcattgcta cccagacaaa gagctgccgc
     1081 cagcgggcgc attgccgttc cggcgtttcc gaggaacaac tccagggcac cttctgcgtg
     1141 taatggaccg ccgttaccga taatttcgca acgcgtacga tcggctgaaa gcgtatagct
     1201 tacccctaac gctgttaatg cattcagcat atggcgcacg tcatcgctat ccagcagatt
     1261 ggttaatact gttttgccgt gtgctaatgc cgccagcaat aaagcgcggt tagaaacgct
     1321 cttggaacca ggcagattaa tagtgccatc gacacgagcg atgggttgta acgtcaggga
     1381 ttccatgaac tcaactctct acaacagaaa taaaaacccc acaggctggc tgtggggatt
     1441 aagcaaaatt tcggcattag ccgtgacggc gttcgaactc aaccatgaag tctgtcaggg
     1501 ctttaacgcc ttccagcggc atggcgttat aaatagacgc gcgcattccg ccgaccacac
     1561 ggtgaccttt cagcgcatga aggccagcag caaaagactc ttcaaggaac aatttgtcaa
     1621 gcgcactgtc cgccaactgg aacggcacgt tcatccgcga acggttagct ttcgccacgt
     1681 cattgcggta gaaattgctg ttatcaatca ccccatacag cagttctgct ttttgctgat
     1741 tgattttatc cattgcagct acaccgccgt tcgctttcag ccatttaaag accagaccag
     1801 acagatacca ggcaaatgtc ggcggcgtgt taaacatgga gtcgttatcg ttgagaatgg
     1861 aataatccag aatcgacgga cacgcgatat tcgctttgcc cagcaaatct tcacgaacga
     1921 tgacgattgt caggccagcc gggccgatat ttttctgcgc gccagcgtaa atcacgccat
     1981 aacggctgac gtcaatcgga cgggaaagaa tggttgaaga gaagtcggcg gcgaccacca
     2041 catctttgcc gaagtctggc gtttcgtcga tagcgatacc gtcgatggtt tcattcgggc
     2101 agtaatgcat ataagcagca ttatcagaga gttgccattc gcgcattggc ttaaccgcgc
     2161 gcagaccatc aacagtcact ttggcgtcaa agacattagg cgtgcagtat tttttcgctt
     2221 ctttaatggc acttgccgcc cagtaaccgg catcaacata atctgcggtg gttttatcac
     2281 cgagaatatt cagcggaaca gcagcaaact gaccgcgacc gccgccatgg cagaacaata
     2341 ccttgtagtt ggaggggaca ttaagaagat cgcgaaaatc cttctcggct tcctctgcaa
     2401 cctgaatgaa ctctttgcca cggtgactca cttccatcac cgacgtacca agaccgttcc
     2461 agtcgcgcag ttcctgttga gcctgtttaa gcacctctgc cggtagcatt gccggaccag
     2521 aactaaaatt gaagatttga gccatttccc ctcaccacgt tgcgttgcta ttaacccgcg
     2581 gtcaatcgat tgcgaccaca ggtaatacat tattcctgtg gctatcggtt ttatcattca
     2641 gtgacacaca tcgcaatgag taaaaccgtt gagggcaaaa atgtggcttg catcacacaa
     2701 aagaatctct cggattatta gcaaaaagcc gatgttttta ctgtcacgtc acccgttttg
     2761 cccgacttgc cttcgtccat tctggatttg cacagcgcgg acataattgt ttttcccccg
     2821 cgttcatatg aatgatttca ctgcaacgaa tgcaggcata gtccaccgac tcgggtacca
     2881 ctttgtaacg ctgagtacag tcactgggta aaataagtaa gcccggtttc accttgtcat
     2941 cttcaaagaa atagacactg atttgcccgt tggtttcgag aatcgccaga cgtacttgcc
     3001 ccaactgctc tacaccgcgc aatcgtagct ccataaagaa ctcaaattcc gtcatgttgg
     3061 agttattgag tttcgaccag gccagctcgc catcttcaat aatgacaacc ggcttgcctt
     3121 ccagaagatc ttccagtttt tcactgtgcg ccatcaacca cattaccagg cggtataaca
     3181 acgccagaat aataaagacg ataagtaccg ggaccatcgg cacatcatca taaaacgcca
     3241 catctcccgc cgctgatccc agcgtcagaa tgattaacac ttcaaaaagc gacatctgcc
     3301 gcacaccgcg tcttccggtc attttgagga acaaaaaaac cagtacaaag gtataaagac
     3361 tacgtagtgc cacttcgcca aggaaatcaa aaggcacttt atcaaatgcc atacggtgga
     3421 gatcgaacgc cttcatttat atgctcttat cgtggtttat ccgctaaaag catagtcaac
     3481 agatcatcgg catggcggcg gtgatagcgc ctttgagata aaacccgtat tattgcgcgc
     3541 tttccgtacg actaaagtga ttttcatgac gcaaacattt atccccggca aagatgccgc
     3601 actggaagat tccatcgctc gcttccagca aaaactttca gacctcggct ttcagattga
     3661 agaggcctcc tggctgaatc cggtgcctaa cgtctggtct gtacatattc gcgacaaaga
     3721 gtgcgcactg tgttttacca acggtaaagg cgcaaccaaa aaagcggcgc tggcttctgc
     3781 actcggtgaa tatttcgagc gtctctcaac caactacttt tttgctgatt tctggctggg
     3841 cgaaaccatc gccaacggtc cgttcgtgca ttatcccaac gaaaaatggt tcccactgag
     3901 cgaaaatgac gatgtgccag aagggctgct cgatgaccgt ctgcgcgcat tttacgatcc
     3961 ggagaatgaa ctggccggca gtatgctgat tgacctgcaa tccggtaacg aagatcgtgg
     4021 tatttgcggt ctgccgttta cgcgtcagtc tgataatcag accgtttata ttccgatgaa
     4081 tatcattggt aacctgtatg tttctaacgg tatgtccgct ggcaataccc gtaacgaagc
     4141 acgcgttcag gggttgtccg aagttttcga acgctacgtg aaaaaccgca ttattgctga
     4201 aagcatcagc ttgccggaaa tcccggcaga cgtgctggcg cgttacccag cggtggttga
     4261 agccatcgaa acgctggaag cagaaggttt cccaatcttt gcttatgatg gttcgcttgg
     4321 cggccagtat ccggtgattt gcgtggtact gttcaatcct gctaacggta cctgctttgc
     4381 ctctttcggt gcgcatcctg attttggcgt agcactggaa cgtaccgtga ccgagctgct
     4441 gcaaggtcgt ggcctgaaag atttggatgt gtttactccg ccaaccttcg atgatgaaga
     4501 agttgctgaa cataccaacc tcgaaacgca ctttatcgat tccagcggtt taatctcctg
     4561 ggacctgttc aagcaggatg ccgattatcc gtttgtggac tggaatttct ccggcactac
     4621 ggaagaagag tttgctacgc tgatggctat cttcaacaaa gaagataaag aagtttatat
     4681 tgccgattac gagcatctgg gcgtttatgc ttgccgaatt atcgtgcctg gcatgtccga
     4741 tatttatccg gctgaagatc tgtggctggc gaataacagt atgggcagcc atttacgtga
     4801 aacgattctt tcgttaccag gcagcgagtg ggaaaaagaa gattacctga acctcatcga
     4861 gcaactggat gaagaaggtt ttgatgactt tacccgcgtg cgtgagctgt tgggtctggc
     4921 gaccgggtcg gataacggtt ggtacaccct gcgtatcggt gaattaaaag ccatgctggc
     4981 gctggctggt ggcgatctgg aacaggctct ggtctggacc gaatggacga tggagtttaa
     5041 ctcatcggta ttcagcccgg aacgcgccaa ctattatcgc tgcctgcaaa cgttgttatt
     5101 actggcgcag gaagaagatc gccagccgct gcaatatctg aatgcgtttg ttcgcatgta
     5161 cggcgcagat gccgtggaag ccgccagtgc ggcaatgagc ggcgaagcgg cgttttatgg
     5221 tttgcaacca gtagatagcg atctgcacgc gtttgctgca catcagtcgt tgttgaaggc
     5281 ctacgaaaag ctgcagcgcg ccaaagcagc attctgggca aaataaaatc aaatagccta
     5341 cgcaatgtag gcttaatgat tcgtctgagt tatattacgg ggcgtttttt taatgccccg
     5401 ctttac
//
LOCUS       DAZFLD010000254         5385 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.256, whole genome shotgun sequence.
ACCESSION   DAZFLD010000254 DAZFLD010000000
VERSION     DAZFLD010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5385
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.256"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..789
                     /locus_tag="ACHKBL_004344"
     CDS             <1..789
                     /locus_tag="ACHKBL_004344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HFO5821117.1"
                     /translation="LEGDLLRTEVAGMSVTAGIYGAAGHSSVDVKDDDASRAGTVRDD
                     AGSLGGYLNLTHTSSGLWADIVALGTRHSMKASTDNNDFRARGWGWLGSLETALPFSI
                     TDNLMLEPQLQYTWQGLSLDDGQDNAGYVKFGYGSAQHVRAGFRLGSHNDMTFGEGTS
                     SRDTLRDSTKHGVSELPVNWWVQPSVIRTFSSRGDMSMGTAAAGSNMTFSPSRNGTSL
                     DLQAGLEARVRENITLGVQAGYAHSVSGSSAEGYNGQATLNVTF"
     gene            910..3426
                     /locus_tag="ACHKBL_004345"
     CDS             910..3426
                     /locus_tag="ACHKBL_004345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5821118.1"
                     /translation="MLQIVGALILLIAGFAILRLLFRALISTASALAGLILLCLFGPA
                     LLAGYITERITRLFHIRWLAGVFLTIAGMIISFMWGLDGKHIALEAHTFDSVKFILTT
                     ALAGGLLAVPLQIKNIQQNGITPEDISKEINGYYCCFYTAFFLMACSACAPLIALQYD
                     ISPSLMWWGGLLYWLAALVTLLWAASQIQALKKLTCAISQTLEEQPVLNSKSWLTSLQ
                     NDYSLPDSLTERIWLTLISQRISRGELREFELADGNWLLNNAWYERNMAGFNEQLKEN
                     LSFTPDELKTLFRNRLNLSPEANDDFLDRCLNGGDWYPFSEGRRFVSFHHVDELRICA
                     SCGLTEVHHAPENHKPDPEWYCSSLCRETETLCQEIYERPYNSFISDATANGLILMKL
                     PETWSTNEKMFASGGQGHGFAAERGNHIVDRVRLKNARILGDNNARNGADRLVSGTEI
                     QTKYCSTAARSVGAAFDGQNGQYRYMGNNGPMQLEVPRDQYAGAVETMRNKIREGKVP
                     GVTDPAEASRLIRRGHLTYTQARNITRFGTIESVTYDIAEGSVVSLAAGGISFALTAS
                     VFWLSTGDRDATLQTAAVQAGKTFTRTLAVYVTTQQLHRLSVVQGMLKHIDFSTASPT
                     VRQALQKGTGAGNISALNKVMKGTLVTSLVLVAVTTGPDMIKMLRGRISGAQFIRNLA
                     VASSGVAGGAVGSVAGGILFSPLGPFGALTGRVAGGVLGGMIASAVSGKIAGALVEED
                     RVKILAMIQEQVTWLAGSFLLTGHEIENLNANLARVIDQNALEIIFAAGIQQRAATNM
                     LIKPLVVSIIRQRPVMEYDASHLGKMVNRQEEALLPELPA"
     gene            3502..3963
                     /locus_tag="ACHKBL_004346"
     CDS             3502..3963
                     /locus_tag="ACHKBL_004346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IrmA family protein"
                     /protein_id="HFO5821119.1"
                     /translation="MIHLFKTCMITAFILGLTWSAPLRAQDQRYISIRNTDTIWLPGN
                     ICAYQFRLDNGGNDEGFGPLTITLQLKDKYGQTLVTRKMETEAFGDSNATRTTDAFLE
                     TECVENVATTEIIKATEESNGHRVSLPLSVFDPQDYHPLLITVSGKMLTDP"
     gene            4099..4188
                     /locus_tag="ACHKBL_004347"
     CDS             4099..4188
                     /locus_tag="ACHKBL_004347"
                     /inference="COORDINATES: protein motif:HMM:NF017787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HFO5821120.1"
                     /translation="MTAYRNVFIQDDPGMHFRRVIRNAEGQRR"
     gene            4366..5187
                     /locus_tag="ACHKBL_004348"
     CDS             4366..5187
                     /locus_tag="ACHKBL_004348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HFO5821121.1"
                     /translation="MRLVSRFGYAANQIRRDRPLTHEELMHHVPGIFGEEKHTSRSQN
                     YTYIPTITVLESLQREGFQPFFACQTRVRDPGRRGYTKHMLRLRRAGEINGEHVPEII
                     LLNSHDGTSSYQMLPGYFRFVCQNGCVCGQSLGEVRVPHRGNVVQKVIEGAYEVVGVF
                     DRIEEKRDAMQSLVLPPPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLW
                     SAYQTIQENMLKGGISGRSAKGKRIHTRAIHSIDTDIKLNRALWVMAETLLESMR"
     gene            5187..>5385
                     /locus_tag="ACHKBL_004349"
     CDS             5187..>5385
                     /locus_tag="ACHKBL_004349"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5821122.1"
                     /translation="MKHIPEGVIQAPVSPDAPEALPGCRSISDDDLCAWCTRLLYRPG
                     ELSLCRLSTGNGIWPSVCDRNG"
BASE COUNT         1274 a         1410 c         1480 g         1221 t
ORIGIN      
        1 ctggagggtg acctgctgag aacagaggtt gccggtatgt ctgtgaccgc agggatatat
       61 ggtgctgcag gccattcttc cgttgatgtt aaggatgatg acgcctcccg tgccggcacg
      121 gtccgggatg atgccggcag ccttggcgga tacctgaatc tgacacatac atcctccggc
      181 ctgtgggctg acattgtggc cctgggaacc cgccacagca tgaaagcgtc aacggacaat
      241 aacgacttcc gcgcccgggg gtggggctgg ctgggttcac tggaaaccgc tctgcccttc
      301 agtatcactg acaatctgat gctggagccg caactgcagt acacctggca gggactctcc
      361 ctggatgacg gccaggataa cgccggttat gtgaagttcg ggtatggcag tgcacaacat
      421 gtgcgtgccg gtttccgtct gggcagccac aacgatatga cctttggtga aggcacctca
      481 tcccgtgaca ccctgcgcga cagtacaaaa cacggtgtga gtgaactgcc tgtgaactgg
      541 tgggtacaac cttctgttat ccgcaccttc agctcccggg gggacatgag catgggtaca
      601 gctgcagccg gcagtaacat gacgttctca ccgtcccgga atggcacgtc actggacctg
      661 caggccggac tggaagcccg tgtccgggaa aatatcaccc tgggcgttca ggccggttat
      721 gcccacagcg tcagcggcag cagcgctgaa gggtataacg gtcaggccac actgaatgtg
      781 accttctgac agaaccatcg cctctctgtg gtcccggtca tcatgaccgg gacccggacc
      841 ggcgcaacgg atcttcaacg ccacattcgc tggcattaac aataacatga tattcatcac
      901 ggagtgacta tgttacagat agtcggcgcg ctgatcctgc tgatcgcagg atttgccatt
      961 cttcgccttt tgttcagagc attaatcagc acggcttctg cgctggcagg gctcatattg
     1021 ctgtgtctgt tcggcccggc cttactggct ggctatatca ccgaacgcat aacccggttg
     1081 ttccatattc gctggctggc aggcgtattt ctgacgattg ccggaatgat catcagcttc
     1141 atgtggggac ttgatggtaa acatatcgcg ctggaggctc acacctttga ctctgtgaaa
     1201 tttattctga ccaccgctct cgccggtggt ctgctggctg ttcccctgca gatcaaaaac
     1261 attcagcaga acgggatcac accagaagat atcagcaagg aaattaacgg gtattactgc
     1321 tgtttttata ctgccttttt ccttatggcg tgttctgcat gcgcaccatt gatcgcgtta
     1381 cagtacgata tttcaccgtc actgatgtgg tggggcgggt tgttgtactg gctggctgca
     1441 ttagtgacgc tgctatgggc ggccagccag atccaggcgc tgaaaaaact gacctgtgcc
     1501 atcagccaga cactggaaga acaaccggtg ctcaacagta aatcgtggct gaccagtttg
     1561 caaaacgatt acagccttcc tgactcactg acggagcgca tctggctgac gctcatttct
     1621 caacggattt cccggggaga gctgagggaa tttgaactgg cagacggaaa ctggttactg
     1681 aacaatgcct ggtatgaaag aaacatggca gggtttaacg aacagttgaa agagaacctg
     1741 tcattcacac ctgatgaact gaaaacgctc ttccggaacc gcctgaattt atcaccggaa
     1801 gcgaatgacg attttctcga tcgttgcctg aacggcggtg actggtatcc cttttcagaa
     1861 ggtcgccgtt ttgtatcatt ccatcacgtg gatgagcttc gtatctgtgc ctcctgcggg
     1921 ctgacagaag tacatcatgc cccggaaaat cataagccgg atccggaatg gtactgctcc
     1981 tctctttgtc gcgaaacaga aacactgtgt caggaaattt atgaacgccc ttacaacagc
     2041 tttatttccg atgcaacggc gaatggtctg attctcatga aactgccgga aacctggagt
     2101 acaaatgaga aaatgtttgc ttccggaggg cagggacatg ggtttgccgc tgaacgggga
     2161 aaccatattg tcgacagagt ccgtctgaaa aacgcacgga tcctcggtga taataatgcc
     2221 agaaatggag cagacagact ggtcagcgga acagaaatcc agacgaaata ttgttcaact
     2281 gcagcccgta gcgtcggtgc ggcattcgac ggacaaaacg gacagtatcg ttacatggga
     2341 aataatggcc ccatgcaact ggaagtcccc cgtgatcagt atgccggcgc tgttgaaacc
     2401 atgaggaata agatccgcga aggtaaagta cccggtgtaa ccgatcccgc agaagcgtcc
     2461 cggctgattc gtcggggaca tctgacttat actcaggccc gtaatatcac ccggttcggg
     2521 accatcgaat cggtcactta tgatattgcc gagggttcgg ttgtcagtct ggcggccgga
     2581 gggatcagtt ttgccctgac ggcatcggtc ttctggctca gcaccggcga tcgcgatgct
     2641 accctgcaga cagctgctgt ccaggcagga aaaaccttca cccgcacact ggctgtctac
     2701 gtcacaaccc agcaactcca ccggctcagt gttgttcagg gtatgctgaa gcatattgat
     2761 ttttcgacgg ccagcccgac tgtccggcag gcgcttcaga aggggaccgg tgcaggaaat
     2821 atcagtgccc tgaacaaagt gatgaagggt acgctggtga catctctggt actggtcgct
     2881 gtcacaaccg gccctgacat gatcaaaatg ttgcggggac ggatctccgg tgcgcagttc
     2941 atcaggaatc ttgccgtggc atcttccggt gtggcaggtg gtgctgtcgg gtcagtggcg
     3001 ggcgggatac tgttcagtcc actgggacca tttggtgcac tgacagggcg tgtggctggc
     3061 ggtgttctgg ggggaatgat tgcctccgct gtatcaggaa aaattgccgg agcgctggtt
     3121 gaagaagatc gcgtcaaaat tctggcaatg attcaggagc aggtgacatg gcttgccggc
     3181 agtttcctgc tgaccggaca tgagattgaa aatctgaacg cgaatctggc ccgtgttatc
     3241 gatcagaatg ctctggagat cattttcgcc gccggtatac aacaacgggc cgcgaccaat
     3301 atgttaatca aaccactggt ggtcagtatc atcaggcaac gccccgtcat ggaatatgat
     3361 gcatcccatc tcggcaagat ggttaaccga caggaagaag cattactccc ggagttaccg
     3421 gcataacggc tattctgcgc cttttacgtt ttatcacctg atttgtcagt gtcatcgtca
     3481 tgactcagag aggtaaatac catgattcac ctgttcaaaa cctgcatgat taccgccttc
     3541 attctggggt taacgtggtc tgccccactc cgggcacagg atcaacgtta catcagtata
     3601 cgcaatacag atacgatatg gctcccggga aatatttgtg cttaccagtt ccggctggat
     3661 aatggcggaa acgatgaagg atttggcccc ctcaccatca ctctgcaact caaagacaaa
     3721 tatggtcaga cgctggtgac cagaaaaatg gaaacggaag cctttggtga cagtaatgcc
     3781 acgcgaacca cagacgcatt tctggaaacg gagtgcgtgg aaaatgtcgc cacaaccgaa
     3841 atcattaaag caactgaaga aagtaacggc catcgtgtca gtctgccgtt atcggttttc
     3901 gatccccagg actaccatcc actgctgatt accgtttccg gaaaaatgtt aactgatccc
     3961 tgaagtcagt ttcatgcttc agcctctgcc acaacgtatg gcagaggcta tttatcagga
     4021 gaaaccagat atgccgggtt acacacagag tatgtcctgg ctgaaggatc tttctcatac
     4081 gggcaggcgg ttgctgtcat aaccgcttac cgcaatgtgt ttatccagga tgacccggga
     4141 atgcatttcc gtcgggttat ccgcaacgcg gaaggtcagc gacgctgacg ctgccggaac
     4201 tctgaagctg atgccggaaa acagcttaat gcgtggctcg ccagtggagg gcttctcaga
     4261 caataaacgt cattgatttc gtccatcagg ccgcgtcttc tccgggagac gcggcttttt
     4321 catttattgc accagtaaat cttaaccacc gataaggagc aaagtatgcg attagtcagt
     4381 cgttttggtt atgctgcaaa ccagatacgc cgtgaccgtc cgctgacaca tgaagagctg
     4441 atgcaccatg tgccggggat tttcggggaa gagaaacaca catcccgaag ccagaactat
     4501 acgtacatcc ccaccatcac cgtactggaa agtctgcagc gggaaggctt ccagcccttt
     4561 ttcgcctgcc agacccgtgt gcgcgacccg ggccgccggg gatacacaaa acacatgctg
     4621 cgtctgcggc gggccggaga gataaacgga gaacatgtcc ctgaaattat tctgctcaac
     4681 tcccatgacg gtacctccag ctaccagatg ctgccgggtt acttccggtt cgtctgccag
     4741 aacgggtgcg tctgtggtca gtctctgggg gaagtgcgtg ttccacaccg gggaaatgtg
     4801 gtgcagaaag tcattgaagg ggcttacgag gtggtgggcg tttttgaccg gattgaagag
     4861 aaacgtgatg ccatgcagtc gctggtcctg ccgccaccgg cacgccaggc gctggcacag
     4921 gcggcactga cttaccgtta tggtgacgaa catcagcccg tcaccaccgc cgacattctg
     4981 acgccacgac gtcgggagga ttacggtaaa gacctgtgga gcgcatatca gaccatccag
     5041 gagaatatgc tgaaaggcgg gatttccggt cgcagtgcaa aaggaaaacg tatccacacc
     5101 cgtgccattc acagcatcga caccgatatt aagctcaacc gcgcattgtg ggtgatggca
     5161 gaaacgctgc tggagagcat gcgctgatga aacatatccc tgaaggcgtg attcaggcac
     5221 ccgtatcacc cgacgccccg gaggcgctgc cgggatgtcg ttccatttct gatgatgacc
     5281 tgtgcgcctg gtgtacccgt ctgctttacc gtcccggaga actcagtctg tgcagactga
     5341 gtacaggcaa tggcatctgg ccatccgtct gtgaccggaa cggtt
//
LOCUS       DAZFLD010000255         5357 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.257, whole genome shotgun sequence.
ACCESSION   DAZFLD010000255 DAZFLD010000000
VERSION     DAZFLD010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5357
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.257"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            184..1980
                     /locus_tag="ACHKBL_004350"
     CDS             184..1980
                     /locus_tag="ACHKBL_004350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016159766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aryl-sulfate sulfotransferase"
                     /protein_id="HFO5821123.1"
                     /translation="MFDKYRKTLVAGTVAITLGLSASGVMAAGFKPAPPAGQLGAVIV
                     DPYGNAPLTALVDLDSHVISDVKVTVHGKGEKGVEISYPVGQESLKTYDGVPIFGLYQ
                     KFANKVTVEWKENGKVMKDDYVVHTSAIVNNYMDNRSISDLQQTKVIKVAPGFEDRLY
                     LVNTHTFTAQGSDLHWHGEKDKNAGILDAGPATGALPFDIAPFTFIVDTEGEYRWWLD
                     QDTFYDGRDRDINKRGYLMGIRETPRGTFTAVQGQHWYEFDMMGQVLEDHKLPRGFAD
                     ATHESIETPNGTVLLRVGKSNYRRDDGVHVTTIRDHILEVDKSGRVVDVWDLTKILDP
                     KRDALLGALDAGAVCVNVDLAHAGQQAKLEPDTPFGDALGVGPGRNWAHVNSIAYDAK
                     DDSIILSSRHQGVVKIGRDKQVKWILAPSKGWEKPLASKLLKPVDANGKPITCNENGL
                     CENSDFDFTYTQHTAWISSKGTLTIFDNGDGRHLEQPALPTMKYSRFVEYKIDEKKGT
                     VQQVWEYGKERGYDFYSPITSIIEYQADRNTMFGFGGSIHLFDVGQPTVGKLNEIDYK
                     TKEVKVEIDVLSDKPNQTHYRALLVRPQQMFK"
     gene            1998..2666
                     /locus_tag="ACHKBL_004351"
     CDS             1998..2666
                     /locus_tag="ACHKBL_004351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016159765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HFO5821124.1"
                     /translation="MSKLGISSLFKTILLTAALAVSFTASAFTEGTDYMVLEKPIPNA
                     DKTLIKVFSYACPFCYKYDKAVTGPVSEKVKDIVAFTPFHLETKGEYGKQASEVFAVL
                     INKDKAAGISLFDANSQFKKAKFAYYAAYHDKKERWSDGKDPAAFIKTGLDAAGMSLA
                     DFEAALKEPAVQETLEKWKASYDVAKIQGVPAYVVNGKYLIYTKSIKSIDAMADLIRE
                     LASK"
     gene            2681..3352
                     /gene="dsbI"
                     /locus_tag="ACHKBL_004352"
     CDS             2681..3352
                     /gene="dsbI"
                     /locus_tag="ACHKBL_004352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000511507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide oxidoreductase DsbI"
                     /protein_id="HFO5821125.1"
                     /translation="MGIKGMWKDLRTSPVDTLVRWQEQRLLWLLMAVAMGALIILAHS
                     FFQIYLYMAPCEQCVYIRYAMFVMVIGGLVAAINPKNIILKLIGCVMAFYGSILGLKF
                     SLKLNDIHHAVHNPDPDSLFGVQGCSTDPTFPFNLPLAQWAPNWFKPTGDCGYDAPIV
                     PDGVTLSSTQQWFVEMYQQSEGWYLLPPWHFMNMAQACMLAFGMCLVLLVIMSGAWAL
                     KIVRG"
     gene            complement(3398..4186)
                     /gene="ygiD"
                     /locus_tag="ACHKBL_004353"
     CDS             complement(3398..4186)
                     /gene="ygiD"
                     /locus_tag="ACHKBL_004353"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HFO5821126.1"
                     /translation="MSSTRMPALFLGHGSPMNVLEDNLYTRSWQTLGMTLPRPQAIVV
                     VSAHWFTRGTGVTAMETPPTIHDFGGFPQALYDTHYPAPGSPALAQRLVELLAPIPVT
                     LDKEAWGFDHGSWGVLIKMYPDADIPMVQLSIDSSKPAAWHFEMGRKLAALRDEGIML
                     VASGNVVHNLRTVKWHGDSSPYPWATSFNEYVKANLTWQGPVEQHPLVNYLDHEGGAL
                     SNPTPEHYLPLLYVLGAWDGQEPITIPVDGIEMGSLSMLSVQIG"
     gene            4329..5102
                     /gene="zupT"
                     /locus_tag="ACHKBL_004354"
     CDS             4329..5102
                     /gene="zupT"
                     /locus_tag="ACHKBL_004354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HFO5821127.1"
                     /translation="MSVPLILTILAGAATFIGAFLGVLGQKPSNRLLAFSLGFAAGIM
                     LLISLMEMLPAALAAEGMSPVLGYGMFIFGLLGYFGLDRMLPHAHPQDLMQKSVQPLP
                     KSIKRTAILLTLGISLHNFPEGIATFVTASSNLELGFGIALAVALHNIPEGLAVAGPV
                     YAATGSKRTAILWAGISGLAEILGGVLAWLILGSMISPVVMAAIMAAVAGIMVALSVD
                     ELMPLAKEIDPNNNPSYGVLCGMSVMGFSLVLLQTAGIG"
     gene            complement(5160..5330)
                     /gene="yqiD"
                     /locus_tag="ACHKBL_004355"
     CDS             complement(5160..5330)
                     /gene="yqiD"
                     /locus_tag="ACHKBL_004355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqiD"
                     /protein_id="HFO5821128.1"
                     /translation="MFIAWYWIVLIALVVVGYFLHLKRYCRAFRQDRDALLEARNKYL
                     NSTREETAEKVE"
BASE COUNT         1394 a         1322 c         1284 g         1357 t
ORIGIN      
        1 cctactatcc attaaacata aatatttgtt tcaccgaata acaacatatt taaaagaaac
       61 acaaaagcat tctcactctt tattgaatta atatttatat tcacgtaacg aatttattag
      121 caccctctca acagtcgata ttcatcatta ttatatattt gcgttatata aggagatata
      181 tccatgtttg ataaatatag aaaaacactc gtagccggaa ctgtggcgat aaccctgggt
      241 ttgtcagcat cgggggtgat ggctgcgggt tttaaaccag cgccgcctgc cgggcaactg
      301 ggtgcggtca ttgtcgatcc ctacggcaat gcaccactga ccgctttggt tgacttagat
      361 agccatgtta tttctgacgt caaagttacc gtccatggga agggcgaaaa aggtgtagaa
      421 atcagctatc ccgtgggtca ggaatcacta aaaacttacg atggtgtacc gatttttggt
      481 ctttatcaga aatttgctaa caaagtgacc gttgagtgga aagaaaacgg caaggtcatg
      541 aaagatgatt atgtggtgca cacttcggcc atcgtcaata attacatgga taaccgctct
      601 atatccgatt tacaacagac caaagttatt aaagtcgcac cgggttttga agatcgcctc
      661 tatctggtta atacccacac ctttaccgcc caaggttccg atctccactg gcatggtgag
      721 aaagataaaa atgccggtat ccttgatgcg ggtccggcaa ccggcgcact accttttgat
      781 atcgcgccct ttactttcat cgtcgatacc gaaggtgaat accgctggtg gctggatcaa
      841 gacactttct acgatggtcg tgaccgcgat atcaacaagc gtggttatct gatgggtatc
      901 cgcgaaacgc cacgcggcac ctttaccgct gtacaaggtc agcactggta cgagttcgac
      961 atgatggggc aggtgctcga agatcacaaa ctaccgcgcg gatttgctga cgccactcat
     1021 gagtccattg agacgccaaa tggcacggta ctgttgcgcg taggtaagag taactatcgt
     1081 cgcgatgacg gcgtacacgt caccaccatt cgtgaccata tcctcgaagt tgataaatct
     1141 ggtcgcgttg tcgatgtatg ggatctgacc aagatcctcg atccgaaacg cgatgcactg
     1201 ctcggcgcgc tggatgcagg tgcagtttgc gttaacgttg accttgccca tgcaggacaa
     1261 caggcaaaac tggaaccaga tacaccgttc ggcgacgcgc tgggtgtagg gccaggccgc
     1321 aactgggcgc acgtgaactc tatcgcctat gacgcaaaag atgactctat catcctctcc
     1381 tctcgccacc agggtgttgt gaaaattggt cgtgataagc aagtgaaatg gatccttgcg
     1441 ccctctaaag gttgggaaaa accgctggcc agcaagctgc tgaaaccggt tgatgctaac
     1501 ggtaagccaa ttacctgtaa cgaaaatggc ctgtgcgaaa actcagactt cgactttacc
     1561 tacacccagc ataccgcctg gatttccagc aaaggaacgc tcaccatttt tgataatggc
     1621 gatgggcgtc atctggaaca acctgcctta ccaaccatga aatactcccg ctttgtggaa
     1681 tataagattg atgagaagaa aggcaccgtt cagcaagtgt gggaatacgg taaagaacgt
     1741 ggctacgatt tctatagccc gattacctcc atcattgaat atcaagccga ccgtaacacc
     1801 atgtttggct tcggtggttc tattcatttg ttcgatgtcg ggcagccaac cgtcggtaag
     1861 ttgaacgaaa tcgattacaa aaccaaagaa gtgaaagtgg aaatcgacgt gctgtcagat
     1921 aaacccaatc agactcacta tcgtgcactg ttagtccgtc cacaacagat gttcaaataa
     1981 ttaagaaggg aataaaaatg tcgaaattag ggatctcatc actatttaaa accatactgc
     2041 ttaccgcagc tttggctgtt tcatttaccg cttctgcatt taccgaaggc acggattaca
     2101 tggttctgga aaaacctatt ccgaatgccg acaaaacgct gattaaggta ttcagctacg
     2161 cctgcccgtt ctgttacaag tacgacaaag cggttaccgg tccggtatcg gaaaaagtga
     2221 aagatatcgt tgctttcacg ccgttccatc tggaaaccaa aggtgaatac ggcaaacaag
     2281 caagcgaagt ctttgcggtc ttgatcaata aagataaagc agcaggcatt tccttatttg
     2341 atgctaactc tcagtttaag aaagccaagt ttgcctacta cgcggcttat cacgacaaaa
     2401 aagaacgctg gtctgacggt aaagatccgg ctgcttttat caaaaccggt ctggatgccg
     2461 caggtatgag cttggcggat tttgaagctg cactgaaaga accagcggtt caagaaacac
     2521 tggaaaaatg gaaagcctct tatgatgtcg ccaaaatcca gggggttcca gcctatgtcg
     2581 ttaatggtaa gtacttgatc tacaccaaga gcatcaaatc catcgacgcg atggcagacc
     2641 ttatccgtga gttagcaagc aaataacagg gaaacaggct atgggtatta agggaatgtg
     2701 gaaagacctt cgcacatcac cagttgacac actggtcaga tggcaggagc aacgcttgtt
     2761 gtggttgtta atggccgttg cgatgggcgc gttaattatc ctggcgcact ctttcttcca
     2821 gatctatctc tacatggcac cctgtgagca atgtgtatac attcgctacg ccatgtttgt
     2881 gatggttatt ggaggattgg ttgcagcgat caacccaaag aacatcatcc taaagctaat
     2941 tggctgcgtg atggcatttt acggcagcat tttgggactg aagttttcgc tcaaactaaa
     3001 cgatatccac catgcagtac ataatccgga tcccgattca ctgtttggcg tacagggttg
     3061 ctctaccgat ccaacttttc cctttaacct gccgctggca cagtgggcac caaattggtt
     3121 caagcctaca ggggattgtg gctatgacgc accaatcgtt cctgatggcg tcacactgag
     3181 cagcacgcaa cagtggtttg tggaaatgta tcagcaatct gaaggctggt atctgctacc
     3241 accatggcat tttatgaata tggctcaggc ttgtatgctg gcttttggta tgtgtctggt
     3301 gctgctggtc attatgagtg gcgcatgggc gcttaaaatc gtcagaggct aactcagcaa
     3361 cacccgaagc cagtcgttct ggcttcgggt tcttttatta gcctatctgc accgacaaca
     3421 tacttaaact gcccatttct ataccatcga ccggaatggt aattggctcc tgcccatccc
     3481 acgcacctaa gacatacaac aacggcagat agtgctctgg cgttgggttc gataacgcgc
     3541 caccttcatg gtcgagataa ttcaccagag gatgctgctc taccggccct tgccacgtca
     3601 gattcgcttt cacatactca ttaaacgacg tcgcccacgg atacggtgaa ctatcaccgt
     3661 gccacttcac tgtgcgcagg ttatgcacca cgttaccgct ggcgaccaac attattcctt
     3721 catctcgcag cgccgcgagt ttgcgcccca tttcgaaatg ccaggcggca ggtttgctac
     3781 tgtcgatact caactgcacc atcgggatat ccgcgtcagg atacatctta atcagcacgc
     3841 cccaggaacc gtggtcaaag ccccaggctt ctttatccag cgtcaccggg atcggcgcta
     3901 acagctcaac cagacgctgt gccagcgcag gcgaacccgg agcaggataa tgcgtatcgt
     3961 acagcgcctg cgggaagcca ccaaagtcat gaatcgtggg cggcgtctcc atcgcggtca
     4021 ctcctgttcc acgggtaaac cagtgagccg aaaccaccac aatcgcttgc gggcgtggca
     4081 atgtcatccc caacgtctgc cagctgcggg tatacaaatt atcttccagc acgttcatcg
     4141 gactaccgtg acctaaaaac aatgctggca tacgtgttga agacatgatg atatccttaa
     4201 ctaaaggtgt cattttgata tcctcacaat acgcttgttc ggcggagtaa gaacccggat
     4261 aacaatgatg atgatcatca gttattttga cgatctgcct gaaggtgaag atttataagg
     4321 agttgtcgat gtcagtacct ctcattctga ccatactggc gggggcagcc acgtttattg
     4381 gcgcgtttct cggcgttctc gggcaaaaac cctcgaaccg cttactggcg ttttcgctag
     4441 gatttgcggc ggggatcatg ttgctcatct cattaatgga aatgcttcct gccgcactag
     4501 ccgccgaagg aatgtcgcct gtgttgggtt atggaatgtt tatcttcggt ctgcttggct
     4561 attttggcct ggaccgcatg ttgccacatg cgcatccgca ggatttaatg caaaaatcgg
     4621 tgcagccgtt gccaaaatcg atcaagcgca cagccattct gctcactctc ggcatcagtc
     4681 tgcataactt cccggaaggg attgccacct ttgtcacggc gagcagcaac ctggagctgg
     4741 gctttggcat cgcactggcc gttgcgttac acaatattcc tgaaggtctg gcggttgcag
     4801 gcccggttta cgcggcaacg gggtctaaac gcaccgcaat tctttgggcg gggatttctg
     4861 gactggcaga aattcttggt ggtgtgctgg cgtggttaat cctcggtagc atgatttccc
     4921 cggtggtcat ggcggcaatc atggcggcgg ttgcaggaat tatggtggcg ctctcggttg
     4981 atgaattaat gccgctcgcc aaagagattg accctaataa taaccccagc tatggcgtac
     5041 tatgtggaat gtcagtgatg ggattcagtt tagtgctgct acaaacggcg ggaattggtt
     5101 aaaagcaaca tcagaagttg tccagcgcat ggacaacttc tgccagcttc actaaagcct
     5161 tattctacct tttcggctgt ctcttctctc gtactgttta agtatttgtt ccgcgcttca
     5221 agcagtgcgt ctctgtcctg gcgaaacgcc cgacaataac gtttcaaatg caggaaataa
     5281 cccaccacaa caagagcaat caatacaatc cagtaccagg cgataaacat ccttttaccc
     5341 ttaatattta acaatac
//
LOCUS       DAZFLD010000256         5313 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.258, whole genome shotgun sequence.
ACCESSION   DAZFLD010000256 DAZFLD010000000
VERSION     DAZFLD010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5313
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.258"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..818
                     /gene="rcnA"
                     /locus_tag="ACHKBL_004356"
     CDS             <1..818
                     /gene="rcnA"
                     /locus_tag="ACHKBL_004356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nickel/cobalt efflux protein RcnA"
                     /protein_id="HFO5821129.1"
                     /translation="FTTLLQQGNAWFFIPSAILLGALHGLEPGHSKTMMAAFIIAIKG
                     TIKQAVMLGLAATISHTAVVWLIAFGGMVISKRFTAQSAEPWLQLISAVIIISTAFWM
                     FWRTWRGKRNWLENMHEHDHEHHHHDHEDHHDHGHHHHHEHGEYQDAHARAHANDIKR
                     RFDGREVTNWQILLFGLTGGLIPCPAAITVLLICIQLKALTLGATLVVSFSIGLALTL
                     VTVGVGAAISVQQVAKRWSGFNTLAKRAPYFSSLLIGLVGVYMGVHGFMGIMR"
     gene            1037..1375
                     /gene="rcnB"
                     /locus_tag="ACHKBL_004357"
     CDS             1037..1375
                     /gene="rcnB"
                     /locus_tag="ACHKBL_004357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II) efflux transporter accessory
                     subunit RcnB"
                     /protein_id="HFO5821130.1"
                     /translation="MTIKNKMLLGALLLVTSAAWAAPATAGSTNTSGISKYELSSFIA
                     DFKHFKPGDTVPDMYRTDEYNIKQWQLRNLPAPDAGTHWTYMGGAYVLISDTDGKIIK
                     AYDGEIFYHR"
     gene            complement(1457..2491)
                     /locus_tag="ACHKBL_004358"
     CDS             complement(1457..2491)
                     /locus_tag="ACHKBL_004358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative fimbrial-like adhesin protein"
                     /protein_id="HFO5821131.1"
                     /translation="MEIRIMLFIVMMMVMPVSYATCYSELSVQHNLVVQGDFALTQTQ
                     MATYEHNFNDSSCVSTNTITPMSPSDIIVGLYNDTIKLNLHFEWTNKNNITLSNNQTS
                     FTSGYSVTVTPAASNAKVNVSAGGGGSVMINGVATLSSASSSTRGSAAVQFLLCLLGG
                     KSWDACVNSYRNALAQNAGVYSFNLALSYNPITTTCKPDDLLINLESIPVSQLPATGN
                     KATINSKKGDIILRCKNLLGQQNQTSRKMQVYLSSSDLLAGSNTILKGAEDNGVGFML
                     ESNSSPVTFLNITDNSKGYTNLKEIAAKSKITDTTVSIPVTASYYVYDKNKVKSGALQ
                     ATALINVKYD"
     gene            complement(2507..4987)
                     /locus_tag="ACHKBL_004359"
     CDS             complement(2507..4987)
                     /locus_tag="ACHKBL_004359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HFO5821132.1"
                     /translation="MLRMTPLASAIVALLLGIEAYAAEETFDTHFMIGGMKDQQVANI
                     RLDDNQPLPGQYDIDIYVNKQWRGKYEIIVKDNPQETCLSREIIKRLGINTDNFASGK
                     QCLTFEQLVQGGSYTWDIGVFRLDFSVPQAWVEELESGYVPPENWERGINAFYTSYYV
                     SQYYSDYKASGNSKSTYVRFNSGLNLLGWQLHSDASFSKTNSNPGVWKSNTLYLERGF
                     AQLLGTLRVGDMYTSSDIFDSVRFSGVRLFRDMQMLPNSKQNFTPRVQGIAQSNALVT
                     IEQNGFVVYQKEVPPGPFAITDLQLAGGGADLDVSVKEADGSVTTYLVPYAAVPNMLQ
                     PGVSKYDFAAGRSHIEGASKQSDFVQAGYQYGFNNLLTLYGGSMVANNYYAFTLGTGW
                     NTRIGAISVDATKSHSIQDNGDVFDGQSYQIAYNKFVSQTSTRFGLAAWRYSSRDYRT
                     FNDHVWANNKDNYRRDENDIYDIADYYQNDFGRKNSFSANMSQSLPEGWGSVSLSTLW
                     RDYWGRSGSSKDYQLSYSNNLRRISYTLAASHAYDENHHEEKRFNIFISIPFDWGDDV
                     TTPRRQIYMSNSTTFDDQGVASNNTGLSGTVGSRDQFNYGVNLSYQHQGNETTAGANL
                     TWNAPVATVNGSYSQSSTYRQAGASVSGGIVAWSGGVNLANRLSETFAVMNAPGIKDA
                     YVNGQKYRTTNRNGVVVYDGMTPYRENHLMLDVSQSDSEAELRGNRKIAAPFRGAVVL
                     VNFDTDQRKPWFIKALRTDGQPLTFGYEVNDIHGHNIGVVGQGSQLFIRTNEVPPSVN
                     VAIDKQQGLSCAITFGKEIDESRNYICQ"
     gene            complement(5003..>5313)
                     /locus_tag="ACHKBL_004360"
     CDS             complement(5003..>5313)
                     /locus_tag="ACHKBL_004360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial assembly protein"
                     /protein_id="HFO5821133.1"
                     /translation="ALKFAMQNRIKLFYRPAGIAPVNKATFKKLLVNRSGNGLVIKND
                     SENWVTISDVKANNVKVNYETIMIAPLESQSINVKSNNANNWHLTIIDDHGNYISDKI
                     "
BASE COUNT         1425 a         1302 c         1056 g         1530 t
ORIGIN      
        1 aatttacaac acttcttcag caaggaaacg cctggttctt catccccagc gccatcttac
       61 ttggtgcgct tcatggcctg gaaccggggc actcaaaaac gatgatggcg gcgtttatca
      121 tcgccatcaa aggcaccatt aaacaggcgg tgatgctcgg actggcagca actatttcgc
      181 ataccgcagt agtctggtta attgcctttg gcgggatggt gatcagcaaa cgctttactg
      241 ctcaatcagc agaaccgtgg ctccagttga tttccgcagt gatcattatt agcaccgcgt
      301 tctggatgtt ctggcgtacc tggcgcggca aacgcaactg gctggagaat atgcacgagc
      361 atgatcatga gcatcatcac cacgatcacg aagatcacca cgaccatgga catcatcacc
      421 atcacgaaca tggcgagtat caggatgccc atgcacgagc ccatgccaat gacattaaac
      481 gacgctttga tggtagagag gtcaccaact ggcaaatttt gttatttggc ttaaccggtg
      541 gccttatccc ctgcccggca gcaattaccg tgctgttgat ttgcattcag ttgaaagccc
      601 tgacactggg cgcaacactg gtcgtcagtt tcagcatcgg cctggcgtta acgcttgtca
      661 ccgtaggcgt tggcgcagca atcagcgttc agcaggtcgc aaaacgctgg agcggattta
      721 acactctcgc taaacgcgct ccctattttt ccagtctgtt gattggctta gttggtgtgt
      781 atatgggcgt acatggcttc atgggcataa tgcgataact caatccttta ggcttgcatg
      841 ttatcttcag cccaggatga ttcttaaatc agctatttcc gctgactctc tgctcgctaa
      901 ggatgctttt agggcatcct ttcatttaca ctttttacga aatcatggga tcactaacaa
      961 aatatcgctt gtcagttata ttgtatggca ggaaagatat gcgactgata ttacagatcc
     1021 ccaaagtgga gagtttatga ccattaaaaa taagatgttg ctgggtgcgc ttttgctggt
     1081 taccagtgcc gcctgggccg caccagccac cgcgggttcg accaatacct cgggaatttc
     1141 taagtatgag ttaagcagtt tcattgctga ctttaagcat ttcaaaccag gcgataccgt
     1201 cccagacatg taccgcaccg atgagtacaa cattaagcag tggcagttac gtaacctgcc
     1261 cgcgcctgat gccgggacgc actggaccta tatgggtggc gcgtacgtgt tgatcagcga
     1321 caccgacggt aaaatcatta aagcctacga cggtgagatt ttttatcatc gctaaaaaaa
     1381 gccccctcat catgaggggg aaatgcagac accttgttat tttttattat tcgccacttg
     1441 ctcgtcttgc ttattattag tcgtatttca cattgattaa tgcggttgcc tgtagtgcac
     1501 cagattttac tttattttta tcataaacat agtaactggc agttactgga attgagaccg
     1561 ttgtatctgt aatttttgac ttcgccgcaa tttcttttaa atttgtgtac cctttgctgt
     1621 tatcagtgat atttaaaaat gtgactggcg aactattgct ttcaagcata aatccgactc
     1681 cattatcttc tgcacctttc aggatcgtgt tactgccggc taacaaatca gaacttgata
     1741 aatacacctg catttttcgt gatgtttgat tttgttgacc taataaattt ttacaacgca
     1801 gaataatatc ccctttttta ctatttattg ttgctttgtt acctgtggct ggtaattgtg
     1861 aaacgggaat actctctaaa ttaattaata aatcgtccgg tttgcaggtt gtggttatcg
     1921 ggttgtatga caatgccaaa ttaaaggaat agacacctgc attttgtgcc aatgcatttc
     1981 tgtagctatt tacacatgca tcccatgact tgccacctaa taaacacagt agaaattgta
     2041 ctgcggcact cccgcgtgtc gatgatgaag cactggataa tgttgcaaca ccattaatca
     2101 tcactgaacc gccgcccccc gcagaaacat tcacttttgc attactggcc gcaggtgtca
     2161 ccgttaccga ataaccactg gtgaaactgg tctgattatt tgacaacgta atgttgtttt
     2221 tattggtcca ttcaaaatgt aaatttaatt taatggtatc gttataaagt ccaacaataa
     2281 tatccgacgg gctcataggt gtgatagtat ttgtactcac gcatgacgaa tcattaaaat
     2341 tatgctcata tgtcgccatt tgtgtttgag taagtgcaaa atccccctga acaaccaaat
     2401 tgtgctgaac agataactca ctataacatg tcgcatagct cacaggcata accatcatca
     2461 ttactataaa tagcattatg cgaatttcca tagaaccacc tgactattac tggcaaatat
     2521 aatttctact ttcatcaatc tctttaccga aggtgattgc gcatgaaagc ccttgttgct
     2581 tatcaattgc cacattaacc gatggcggta cttcattggt gcgaataaat aactggcttc
     2641 cctggccgac aacaccaata ttatgaccat ggatatcatt gacttcataa ccaaacgtta
     2701 atggttgccc atccgttctt aacgctttta taaaccaggg cttgcgctga tcggtatcaa
     2761 aattaaccag tacaaccgcg ccgcgaaaag gggcggcaat tttccggttg ccacgtaatt
     2821 ctgcttcgct atcgctttgc gacacatcca gcatcaggtg attttcccga taaggtgtca
     2881 ttccgtcgta taccaccact ccattacggt ttgttgtgcg atatttttgc ccattgacat
     2941 aagcatcttt aattcctggc gcattcatta cagcaaacgt ttcggaaaga cggttcgcca
     3001 gattaacgcc acccgaccag gcgacaatgc cccctgaaac actggctcca gcctgtcgat
     3061 aagtactcga ctgactataa ctgccattca ccgttgccac cggcgcgttc caggttaaat
     3121 tcgctccagc tgtcgtttca tttccctgat gctgataact caggttgacc ccatagttaa
     3181 actgatcccg gcttccaacg gttcctgata atcccgtatt atttgaggca accccctgat
     3241 catcaaacgt cgttgagtta gacatatata tttgccgacg aggcgtcgta acgtcatcac
     3301 cccaatcaaa gggaatcgat ataaaaatat taaaacgttt ctcttcatga tgattctcgt
     3361 cataagcatg gcttgccgcg agggtatagc ttatccttcg caggttgttg gaataactca
     3421 actgataatc cttgctgctg ccgctacgcc cccagtaatc tcgccataac gtacttaagg
     3481 acacagaacc ccagccttct ggcaatgact ggctcatatt ggcagaaaag ctatttttac
     3541 ggccaaaatc gttctggtaa taatcggcaa tgtcatagat atcgttttca tcacggcgat
     3601 aattatcttt attgtttgcc catacgtgat cattaaatgt ccgataatca cgcgacgaat
     3661 aacgccaggc cgccagacca aaacgtgtcg acgtttggct cacaaatttg ttgtaggcaa
     3721 tttgataact ttgcccgtca aacacatcgc cgttgtcttg tatactatgc gacttcgtgg
     3781 catcgacgga aatggcacca atgcgtgtat tccagccagt ccccaaagtg aacgcgtaat
     3841 aattattcgc gaccatcgat ccgccataca gcgtcaataa gttattaaaa ccatactgat
     3901 aacccgcctg aacaaaatca ctttgtttgc tcgccccttc aatatggcta cgacctgccg
     3961 caaaatcata tttcgacacg ccgggttgca gcatattcgg cactgctgca taaggcacca
     4021 gataggtggt taccgaaccg tccgcctctt tcacgctgac atcaagatct gctccaccac
     4081 cggccaactg caaatctgta atcgcaaacg ggccaggagg aacctctttc tgataaacca
     4141 caaaaccatt ctgttcaata gttaccagcg cgttactctg agcaatcccc tgcacccgtg
     4201 gagtaaaatt ttgtttcgag ttaggcaaca tctgcatatc acgaaacaac cgcacaccgc
     4261 tgaagcgaac agaatcaaaa atatcgcttg atgtgtacat atcacccacg cgaagcgtgc
     4321 cgagaagttg ggcaaatcca cgttccagat acagggtatt gcttttccac acccctggat
     4381 tgctatttgt tttactgaaa ctggcatcag aatgcagttg ccaccccagt aagtttaacc
     4441 cgctgttaaa acgtacatat gtactcttgc tattacccga cgctttatag tcgctgtaat
     4501 actgactcac ataataagag gtataaaacg cattaatacc ccgctcccag ttttccggtg
     4561 gaacatagcc actttccagt tcttccaccc acgcctgcgg gacactgaaa tcgagacgaa
     4621 aaactccgat atcccaggta tagctcccac cctgaacaag ttgctcaaat gttaaacatt
     4681 gcttaccgct ggcaaagtta tcggtattaa tgcctaaccg cttgataatt tcccttgata
     4741 aacatgtttc ttgcgggttg tctttaacaa taatctcata tttcccgcgc cattgcttat
     4801 tgacataaat atcgatgtca tactgccccg gcaagggttg attatcatca agacgaatat
     4861 ttgcaacctg ctggtctttc attccaccta tcatgaaatg ggtatcaaag gtttcttcag
     4921 ctgcataagc ttcaatgccg agcaataacg ctacgattgc tgatgcaagt ggggtcattc
     4981 tcaacatagg cagctcctgc aattaaattt tgtcactaat atagttgcca tggtcatcga
     5041 taatggtcag atgccagtta ttcgcattat tacttttgac attaatactc tgactttcta
     5101 agggggcaat cataatagtt tcataattga ctttgacatt attagctttg acatccgaaa
     5161 tcgttaccca attttctgag tcatttttta tcaccaatcc attgccactg cgatttacca
     5221 gtaacttttt aaatgtcgct ttatttaccg gagcaatacc cgctggccgg taaaacaact
     5281 taattctgtt ttgcatcgca aacttcagtg cat
//
LOCUS       DAZFLD010000257         5302 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.259, whole genome shotgun sequence.
ACCESSION   DAZFLD010000257 DAZFLD010000000
VERSION     DAZFLD010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5302
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.259"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(52..1425)
                     /gene="cysG"
                     /locus_tag="ACHKBL_004361"
     CDS             complement(52..1425)
                     /gene="cysG"
                     /locus_tag="ACHKBL_004361"
                     /EC_number="1.3.1.76"
                     /EC_number="2.1.1.107"
                     /EC_number="4.99.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0043115 - precorrin-2 dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051266 - sirohydrochlorin ferrochelatase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siroheme synthase CysG"
                     /protein_id="HFO5821134.1"
                     /translation="MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLDAGARLTVNALA
                     FIPQFTAWADAGMLTLVEGPFDESLLDTCWLAIAATDDDALNQRVSEAAEARRIFCNV
                     VDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPLHLGQVAKYAGQ
                     LRGRVKQQFATMGERRRFWEKLFVNDRLAQSLANNDQKAITETTEQLINEPLDHRGEV
                     VLVGAGPGDAGLLTLKGLQQIQQADVVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHC
                     VPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCNAGIPFSVVPGITAAS
                     GCSAYSGIPLTHRDYAQSVRLITGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQQ
                     KLIEHGMPGEMPVAIVENGTAVTQRVIDGTLTQLGELAQQMNSPSLIIIGQVVGLRDK
                     LNWFSNH"
     gene            complement(1444..2250)
                     /gene="nirC"
                     /locus_tag="ACHKBL_004362"
     CDS             complement(1444..2250)
                     /gene="nirC"
                     /locus_tag="ACHKBL_004362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /protein_id="HFO5821135.1"
                     /translation="MFTDTINKCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIIL
                     IFTLGNLLDPSIRPLVMGATFGIALTLVIIAGSELFTGHTMFLTFGVKAGTISHGQMW
                     AILPQTWLGNLVGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALC
                     NWLVCLAIWMALRTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYT
                     LAGIGHNLLWVTLGNTLSGAVFMGLGYWYATPKANRPVADKFNQTETAAG"
     gene            complement(2377..2703)
                     /gene="nirD"
                     /locus_tag="ACHKBL_004363"
     CDS             complement(2377..2703)
                     /gene="nirD"
                     /locus_tag="ACHKBL_004363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HFO5821136.1"
                     /translation="MSQWKDICKIDDILPETGVCALLGDEQVAIFRPYHSDQVFAISN
                     IDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYEARVKDG
                     VVQLRG"
     gene            complement(2700..5243)
                     /gene="nirB"
                     /locus_tag="ACHKBL_004364"
     CDS             complement(2700..5243)
                     /gene="nirB"
                     /locus_tag="ACHKBL_004364"
                     /EC_number="1.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-nitrite reductase large subunit"
                     /protein_id="HFO5821137.1"
                     /translation="MSKVRLAIIGNGMVGHRFIEDLLDKSDAANFDITVFCEEPRIAY
                     DRVHLSSYFSHHTAEELSLVREGFYEKHGIKVLVGERAITINRQEKVIHSSAGRTVFY
                     DKLIMATGSYPWIPPIKGSDTQDCFVYRTIEDLNAIESCARRSKRGAVVGGGLLGLEA
                     AGALKNLGIETHVIEFAPMLMAEQLDQMGGEQLRRKIESMGVRVHTSKNTLEIVQEGV
                     EARKTMRFADGSELEVDFIVFSTGIRPRDKLATQCGLDVAPRGGIVINDSCQTSDPDI
                     YAIGECASWNNRVFGLVAPGYKMAQVAVDHILGSENAFEGADLSAKLKLLGVDVGGIG
                     DAHGRTPGARSYVYLDESKEIYKRLIVSEDNKTLLGAVLVGDTSDYGNLLQLVLNAIE
                     LPENPDSLILPAHSGSGKPSIGVDKLPDSAQICSCFDVTKGDLIAAINKGCHTVAALK
                     AETKAGTGCGGCIPLVTQVLNAELAKQGIEVNNNLCEHFAYSRQELFHLIRVEGIKTF
                     EELLAKHGKGYGCEVCKPTVGSLLASCWNEYILKPEHTPLQDSNDNFLANIQKDGTYS
                     VIPRSPGGEITPEGLMAVGRIAREFNLYTKITGSQRLAMFGAQKDDLPEIWRQLIEAG
                     FETGHAYAKALRMAKTCVGSTWCRYGVGDSVGLGVELENRYKGIRTPHKMKFGVSGCT
                     RECSEAQGKDVGIIATEKGWNLYVCGNGGMKPRHANLLAADIDRETLIKYLDRFMMFY
                     IRTADKLTRTAPWLENLEGGIDYLKAVIIDDKLGLNAHLEEEMARLREAVVCEWTETV
                     NTPSAQTRFKHFINSDKRDPNVQMVPEREQHRPATPYERIPVTLVEDNA"
BASE COUNT         1235 a         1477 c         1361 g         1229 t
ORIGIN      
        1 ataaagagag ttgtaaacga aaaaggccag aattctctgg ccttggttaa attaatggtt
       61 ggagaaccag ttcaatttat cgcgcaggcc aacaacctga ccaataataa ttagcgatgg
      121 actgttcatt tgctgcgcca gttcgcccag ttgtgtgagc gtaccgtcaa tcacgcgctg
      181 cgtgactgct gttccgtttt cgacaattgc caccggcatt tcgccaggca taccgtgttc
      241 aatcagcttt tgctgaatag tcgcggcctg attcaacccc atatagaaca ccagcgtctg
      301 tttttctgcc gccaggtttt cccagtccag ttcgccaccg gtttttaagt gtccggtaat
      361 taagcgtacg ctctgggcat aatcgcgatg cgtgagcgga atacccgaat aggcagagca
      421 accagaagct gcggtaatac ccggaaccac cgagaacgga atacccgcgt tgcacagtgt
      481 ttccagctct tcgccaccac ggccaaaaat aaacggatcg ccgcctttca gccgcaccac
      541 gcgtttgcct ttttgcgctt cccgtagcag gatctggttt atctcttcct gcggtacgca
      601 gtggtatccc gcgcgtttgc cgacgaaaac gcgatcagca tcgcggcgta ccagattcat
      661 aatatcgtca gaaaccagac ggtcgtagac caccacatct gcctgctgaa tttgttgcag
      721 acctttcagt gtcagcagcc cggcatcgcc cggacctgca ccaaccagca ccacttcacc
      781 gcgatggtcg agcggttcgt tgattaactg ttcggtggtt tcagtaatgg ctttctgatc
      841 gttgtttgcc agcgactgcg ccaggcggtc gttaacgaac aatttctccc agaaacggcg
      901 acgctcaccc atcgtggcga actgttgttt cactcgcccg cgtaattgcc cggcgtattt
      961 cgctacctgg cccagatgca gcggcagcag tgattcgagt ttttcgcgca acaggcgcgc
     1021 cagaaccgga gaggtgccgc cagaggagac cgcgaccatt agcggtgagc ggtcaataat
     1081 tgacggcata ataaagctgg cggctttcgg cgcatcgacc acgttacaga agatgcgacg
     1141 agcttcagcg gcttcactga cgcgctggtt aagcgcgtca tcatccgtcg cagcaatcgc
     1201 cagccagcag gtgtcgagaa ggctttcatc aaatggccct tcgacgaggg ttaacatgcc
     1261 tgcatctgcc catgcggtga actgtggaat aaacgctaat gcattcaccg ttaagcgagc
     1321 gcctgcgtct aacagtaacc ttgctttgcg ttccgcgaca tcaccaccgc cgacaatcag
     1381 acagtcgcga tcgcgtaatt ggcaaaatat aggcaaatga tccacgtaaa aaccccttag
     1441 taattaaccg gcagctgttt cagtttgatt aaatttgtcc gcaaccggac gattcgcttt
     1501 cggcgtagca taccaataac ccaatcccat gaatacggca cctgataaag tattacccag
     1561 cgtcacccac agcaggttat gaccaatacc cgccagcgtg taggcttcgc tgtggttgcc
     1621 gaaccaggag agggcgaaca gcgtcatgtt agcgatggag tgctcgtagc cggacgcgat
     1681 aaatgccagc agacaccacc agatagcgat aaatttcgcc gccccttcag tgcgcagcgc
     1741 catccagata gccaggcaaa ccagccagtt acacaatgca cctttgaaga acagtaccat
     1801 tgccggtgca gtggtttttg ccagcgcgac ggagtgaacg atgctggtat ctaccggcag
     1861 caggctaccg ccgccccagc tatagagcat ggcaacgaag acggaaccga ccagattacc
     1921 cagccaggtt tgcggcagga ttgcccacat ttgcccgtgg ctgatggtgc ccgctttcac
     1981 cccaaaggtg aggaacatgg tgtgtccggt gaacagttca gaaccggcga taatcaccag
     2041 cgttaaggcg ataccaaagg tcgcgcccat caccagagga cgtatggacg gatcgagcaa
     2101 attaccgagc gtgaaaatca ggatgatccc aagacccaca tacgcgcccg ccatggcgga
     2161 gctgacccaa aagccgagcg ggttatttgc cgacaggcgt gcaatgcgcg cagcgttagc
     2221 cgcacactta ttaatagtgt ctgtaaacat ttgattatcc tttaaaaaat aaaaacaaaa
     2281 aaataaaaca aaaagtttaa aaaatgtcct gcaatatacc gaaggtcatt acaggaccat
     2341 gcaaaaaggg gaggcattgc gcctcccgtt aaaacattaa ccgcgcagct gcaccacgcc
     2401 gtctttcact cgtgcttcgt aatgtttgac ggaaaactgt tcgtcttcca tgcacaagcc
     2461 atcacttaag cgaaaacgct gttttttcag cgggctggcg acccacagct cgccctggtg
     2521 ttccgcaatc agcccgcgtg acagcacgct ggactcgaag aacggatcga tgttgctgat
     2581 cgcaaacacc tgatcgctgt gatacgggcg gaaaattgct acttgttcgt cacctaacag
     2641 cgcgcagacg ccggtttcag gcaggatgtc atcgattttg cagatgtctt tccactggct
     2701 catgcgttgt cctccaccag agttaccggg atacgttcat acggcgttgc cggacggtgc
     2761 tgttcgcgct ctggcaccat ctgcacgttc gggtcacgct tatcgctgtt gatgaagtgt
     2821 ttgaagcgag tctgcgcaga cggggtattg accgtttcag tccactcaca cactaccgct
     2881 tcacgcaggc gcgccatctc ttcttccaga tgtgcgttca gccccagttt gtcgtcaatg
     2941 atcaccgctt tcaggtaatc gatgccacct tcgaggtttt ctaaccacgg cgcggtacgc
     3001 gtcagtttgt cggcggtacg gatgtagaac atcatgaagc ggtcgagata tttgatcagc
     3061 gtttcgcgat cgatatccgc tgccaacaga ttcgcatgac gcggtttcat gccgccgtta
     3121 ccgcaaacat acaggttcca gcctttttcg gtggcgataa tacccacgtc tttaccctga
     3181 gcttctgaac attcacgggt acagccggag acaccgaact tcattttgtg cggagtccgg
     3241 atacctttgt agcggttttc cagttccacg ccgaggccga cgctgtcgcc cacgccgtag
     3301 cggcaccagg tgctacccac gcaggttttc gccatacgca gtgctttcgc ataggcatga
     3361 ccggtttcga agccggcttc aatcagctga cgccagatct ccggcagatc gtctttctgt
     3421 gcgccaaaca tcgccaggcg ctgggagccg gtgattttgg tgtagagatt aaattcacgc
     3481 gcgatacgac ctactgccat cagcccttcc ggggtgattt caccgcccgg agaacgcggg
     3541 atcaccgagt aggtgccgtc tttctggatg ttagcgagga agttatcgtt agaatcctgc
     3601 agcggagtat gttccggttt cagaatgtat tcgttccagc aggaggccag cagcgaaccg
     3661 acggtcggtt tacaaacttc acaaccgtag cctttgccgt gtttcgccag cagttcttcg
     3721 aaggttttaa tgccttcaac gcggatcaaa tggaacagtt cctgacgcga ataagcaaag
     3781 tgttcgcaca ggttgttgtt aacttcgatg ccctgtttcg ccagttccgc gttcagtacc
     3841 tgagtgacca gcgggataca gccaccgcag ccagtacccg ctttggtttc agctttcagc
     3901 gccgcaacgg tgtggcagcc tttgttgatg gcagcaatca gatcaccttt ggtgacgtcg
     3961 aagcaggagc agatttgcgc gctgtccggc agtttatcaa caccgataga cggcttaccg
     4021 ctacccgagt gcgccggcag aatcagagaa tccgggtttt ccggcagctc gatagcattc
     4081 agcaccagtt gcagcaggtt gccgtagtcg ctggtatcgc ccaccagtac cgcaccgagc
     4141 agggttttgt tgtcttcgct gacaataagg cgtttgtaga tctctttgct ttcgtcgagg
     4201 taaacgtagc tacgtgcgcc aggtgtgcga ccgtgcgcat caccaatacc gcctacgtct
     4261 acgcccagca gtttcagctt ggcgctaagg tctgcacctt caaaggcgtt ttcgctacca
     4321 agaatatggt caacggcgac ctgcgccatt ttgtagcctg gtgctaccag accaaataca
     4381 cggttgttcc agcttgcgca ttcaccgatg gcgtagatat ccggatcgga agtctggcag
     4441 gaatcattta tgacgatacc cccacgcgga gcaacgtcca gaccacactg ggttgccagc
     4501 ttatcgcgcg gacggatacc ggtagagaag acgataaagt cgacttccag ttcgctgccg
     4561 tcggcaaaac gcatggtttt acgcgcttca acaccttcct gcacaatctc aagagtgttt
     4621 ttgctggtgt gaacgcgcac gcccatactt tcgattttgc gacgcagctg ctcgccgccc
     4681 atctgatcaa gctgttccgc catcagcata ggggcaaatt cgataacgtg ggtttcaatg
     4741 cctaagtttt tcagcgcgcc agcagcttcc agacctaaca ggccgccacc aacaacggca
     4801 ccgcgcttgc tgcgacgagc gcaggattca atggcgttga ggtcttcaat ggtgcgatag
     4861 acaaagcagt cctgagtatc agaaccttta attggcggga tccacgggta ggaaccggtc
     4921 gccatgatca gcttgtcata aaaaacggta cgtccggcgc tggagtgaat caccttctcc
     4981 tgacggttga tggtgatagc gcgttcgccg accagaactt tgatgccgtg tttctcgtag
     5041 aagccttcgc gcaccagcga cagctcttcg gcggtgtggt gagagaagta agacgagagg
     5101 tgtacgcggt cataagcgat gcgcggttct tcacagaaaa cggtaatatc aaagttggcc
     5161 gcatcagatt tatcaagaag atcttcgata aagcgatggc cgaccatacc gttaccgata
     5221 attgcgagtc tgactttgct catttttgcc tcgatttctt ttctattacc gcctacctta
     5281 acgattcagc aaccccgctt at
//
LOCUS       DAZFLD010000258         5302 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.260, whole genome shotgun sequence.
ACCESSION   DAZFLD010000258 DAZFLD010000000
VERSION     DAZFLD010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5302
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.260"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(128..865)
                     /locus_tag="ACHKBL_004365"
     CDS             complement(128..865)
                     /locus_tag="ACHKBL_004365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein mom"
                     /protein_id="HFO5821138.1"
                     /translation="MGNRKQVTSRIISTPELIRYNDNIVGYGSRELRVETISCWLARL
                     VIVNKHYSHRFVNNSYLHLGIFSERELVGVMQWGYALNPNSGARVVTGTQNREYMELN
                     RLWMHDCMPRNSESRAISYALKLIRQLYPQVQWVQSFADERCGCLGVVYQASNFDYVG
                     SHESTFYELDGEWYHEICRNAIKRGGQRGEHLRANIDRASVHKFRQFRYIRFLNKRAR
                     KRLNTKLFKVQPYPKPQTVKTGLKESE"
     gene            complement(819..1019)
                     /locus_tag="ACHKBL_004366"
     CDS             complement(819..1019)
                     /locus_tag="ACHKBL_004366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Com family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFO5821139.1"
                     /translation="MYRNIRCRHCNKLLARASFSYLEVKCPRCKTLNQITSPSATEHP
                     TYTRKSCRGEQKASHIPYHQHA"
     gene            1134..1596
                     /locus_tag="ACHKBL_004367"
                     /pseudo
     CDS             1134..1596
                     /locus_tag="ACHKBL_004367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313023.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1635..1880)
                     /locus_tag="ACHKBL_004368"
     CDS             complement(1635..1880)
                     /locus_tag="ACHKBL_004368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF826 domain-containing protein"
                     /protein_id="HFO5821140.1"
                     /translation="MPEIKGTVTEELVKQALYSEEVNRVLKAQVRKDFEAQIDAYVDE
                     VLARMVGRSPAENSTENEPQPVEQPEPVQPGTDGTMM"
     gene            complement(1916..2095)
                     /locus_tag="ACHKBL_004369"
     CDS             complement(1916..2095)
                     /locus_tag="ACHKBL_004369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1378 family protein"
                     /protein_id="HFO5821141.1"
                     /translation="MTYINVCLLYFCTVVCCLLLVSGGYKIIRNYFRNKIDAAAEEKI
                     KSGAVMPQPKDSGQI"
     gene            complement(2153..4210)
                     /locus_tag="ACHKBL_004370"
     CDS             complement(2153..4210)
                     /locus_tag="ACHKBL_004370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6645 domain-containing protein"
                     /protein_id="HFO5821142.1"
                     /translation="MNIMTSKWVQLSSMPGNFTVKVSGGTAAFLEAPFPPAETKGGMT
                     FADCLISFNTQDCLWVRPVSGDPNVEITGAGIGAVIPLSADVAGTTEPSDWDNAETHT
                     RPAGNETASSSPSWYYVIVIAGQSNASSYGEGLPLPDSYDRPDPRIMQLARRNTQTPG
                     GIPCKYNEIIPADHCLHDVQNMSLLNHPKADLKKGQYGCVGQGLHIAKKVLPVIPADA
                     GILLVPCARGGSAFTTGAVGSFDPASGAAEASLRWGVDTPLYQDLVSRTKAALEANPK
                     NVLLSVVWIQGEGDLKATPAQHGAQFTAMVKKFRADLGAFSGQCIGGSSDSVPWLCGD
                     TTYDWKEKNPAAYETIYSGYKNRASENIHFVPLMHDEFGNKTATNLPEHDPDIASAGY
                     YGAESRGDGNWTTASRATHFSSWARRFLVSDRFASAILKYAGLTSAFIAGVAPDGEAL
                     PQPAPGGKPEAPTGSQPGGSESPHPTSPSLRTLLSLKASAGEAPAQGWKITDGTIQLT
                     DEGKALTVSKQSGKTWTLTHGTDAAGELLKHGGQLTCKFRLSGEVKEGQFALGIYLHT
                     DTVPEGVTMSGTGTPFLMAYYLQTTGGQLNLMHHKTGGNTKLGSFGAYNNQWHTLVLK
                     FTAGSAQVTPVLDGTPGQAFQMVKDALSPAKDTLTLTDVTKGATYGVSFESVVLEVNT
                     PAA"
     gene            complement(4301..4861)
                     /locus_tag="ACHKBL_004371"
     CDS             complement(4301..4861)
                     /locus_tag="ACHKBL_004371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFO5821143.1"
                     /translation="MLIGYIRVSTNDQNTGLQRNALECAGCEQIFEDKMSGKTTQRPG
                     LKRALKKLKPGDTLMVWKLDRLGRSMRHLVALTDELQQQGINFRSLTDNIDTSTPMGR
                     FFFHIMGALAEMERELIVERTRAGLAAARERGRTGGRPRRLTQEQYEQVGRLLAGGVS
                     RRQVALIFDIGLSTLYRYYPASVSEK"
     gene            complement(4875..5061)
                     /locus_tag="ACHKBL_004372"
                     /pseudo
     CDS             complement(4875..5061)
                     /locus_tag="ACHKBL_004372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001449635.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5084..5287
                     /locus_tag="ACHKBL_004373"
     CDS             5084..5287
                     /locus_tag="ACHKBL_004373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO5821144.1"
                     /translation="MADSRYVQSIRRGSRSTIGMQYNIFEVPDGCVLTGLDVAGDGNA
                     TVTAYYRPVQFLIDGSWKTASSA"
BASE COUNT         1342 a         1318 c         1207 g         1435 t
ORIGIN      
        1 atagcgcggc gatttttttg ataccgattt gcatttcatt tgataccgct gaaaaacgcc
       61 gatacgcaac ttatttgata ctaaatcggc gcttttcttt tgagttttag aatgagtttt
      121 taagcgctca ttcactctct ttcaaaccag ttttaactgt ttgtggtttt gggtaaggct
      181 ggactttgaa tagctttgtg ttgaggcgtt ttctggccct cttgttcaga aaccggatgt
      241 aacgaaactg tcggaactta tgcacactgg ctctgtcgat attagccctc agatgttcac
      301 ctcgttgtcc gcctcgttta atggcattcc tgcaaatctc gtgataccac tcgccatcaa
      361 gttcatagaa tgttgattca tggctgccta cataatcaaa attgctggcc tgatacacga
      421 caccaagaca gccacaacgc tcatctgcaa acgactgaac ccactgtacc tgcggataaa
      481 gctgcctgat tagcttgagc gcgtaactga ttgcccttga ttcggagttt ctcggcatac
      541 agtcatgcat ccatagccgg ttaagctcca tatattctcg gttctgcgta cccgttacga
      601 cgcgtgcacc actgtttggg ttaagggcgt aaccccattg cattacgcca acaagttccc
      661 gttctgaaaa tatgcccaga tgaaggtagg aattatttac gaaacggtgg ctgtaatgtt
      721 tattgacgat aaccagtctg gccagccagc aacttattgt ctcaacccgc aactcacggg
      781 agccataacc aacgatattg tcgttatagc gaataagttc aggcgtgctg atgatacggg
      841 atgtgacttg ctttctgttc cccacgacag gatttccttg tgtatgtggg gtgctctgtg
      901 gcgctcggag atgtgatttg attgagggtt ttacagcgcg gacattttac ttccagataa
      961 ctgaaactgg cccgtgccag tagcttattg cagtgtcggc accgtatgtt tcgatacatg
     1021 gccgcataac ctcctgcttt gttgttgatg cctttctcat gcaatcgatc gacaaaaacg
     1081 atcgattcga tttgtttaat tgatacaaca aatagattta aaagtcatca acaatgattc
     1141 agggcggtga ttatttactt aaagaaacga agagttaggt ggtgaaaacg atcataaaat
     1201 gatcatttta ttgacttttt atacagtgaa ggtaggatcg cgcgtatatg tagtatgttt
     1261 caaatcaaca ggatactaca taaaagggaa acccagcata gctggaacta tgctgggtca
     1321 atctgtaaaa acttgcatca atgctttact tcgagctcgt ggcgtatcct atgatattcc
     1381 ccggtctcct gtaaagcaga gatgcataaa aaggagatct ttatcatgga taaagagcct
     1441 gtttgccctg cctgtaagac tcccttacag ctcatctatc gccgctctcg atctcaaaat
     1501 ggcaagacat acaaaccaaa aaatgcttcc tgtttcagga tgctgatatg tcctgcctgt
     1561 tctccccgga gggaaaatac acaaaacgtg cattaacaaa ttcacccggc agggaaacct
     1621 gccggtcggt attgttacat catagtaccg tcagttccgg gctgaaccgg ctctggttgc
     1681 tctacgggtt gaggctcatt ttccgtgctg ttttcagccg gagaacgacc aaccattcgg
     1741 gccagcactt catcaacata tgcgtcgatt tgtgcctcaa aatccttgcg aacctgcgcc
     1801 ttcagcacgc ggttcacttc ttcagaataa agtgcctgtt tgacaagctc ttcagtaacc
     1861 gtgcctttaa tctctggcat gaatattctc ctttcctttt ctggttcaca aaaaattaaa
     1921 tctgaccgga gtctttcggc tgcggcatta cagcaccgct ttttatcttt tcctcggcag
     1981 ctgcatcaat tttatttcga aaataattgc gaataatttt ataaccaccg ctgacaagca
     2041 acagacaaca aaccaccgtg cagaaataca gcaggcagac gtttatatat gtcatataag
     2101 cctcactctt attaactgat gttacagccg gataaattcc cggccgtcac gattatgccg
     2161 caggggtatt aacctcaagg acaacgctct caaaagacac gccataagtt gcccctttgg
     2221 tgacatctgt cagtgtcagc gtatctttgg caggggaaag tgcatctttc accatctgga
     2281 atgcctggcc cggagtgcca tccagcacag gcgttacctg ggcacttccc gccgtaaact
     2341 tcaggacaag tgtatgccac tggttattgt aggctccaaa tgatcccagt ttcgtattcc
     2401 cccctgtttt atggtgcatc agatttaact ggccgcctgt tgtctggaga taatacgcca
     2461 tcaggaacgg tgtgcctgta ccactcatgg ttacaccttc cgggaccgta tcggtgtgga
     2521 ggtaaatacc cagagcaaac tggccttctt ttacttcacc tgacagacgg aatttacagg
     2581 tcaactgacc accatgcttc agaagttcac cggctgcatc agttccatgg gtaagcgtcc
     2641 aggtttttcc gctttgtttg gatacggtca acgctttacc ttcgtccgtc agttggattg
     2701 taccatctgt aattttccag ccctgagccg gggcttctcc tgctgatgct ttaagtgaaa
     2761 gcagcgtacg gagtgacggg gatgtcggat ggggggactc actgcctcct ggctgactac
     2821 ctgtcggcgc ttccggtttc ccacccggcg cgggctgagg cagggcttca ccatcagggg
     2881 ccacaccggc aataaatgca gaggtcagac ctgcatattt aagaattgct gatgcaaaac
     2941 gatctgagac cagaaaacgg cgcgcccagg agctaaaatg ggttgccctg gatgctgttg
     3001 tccagttgcc atctccccgt gattccgcac cataataccc ggcagaagcg atatccgggt
     3061 cgtgctccgg caggttggtc gcggttttat taccgaactc atcgtgcatc agtggaacaa
     3121 agtgaatgtt ctcggaagcc cggtttttat atccgctata aatggtttca tatgccgccg
     3181 ggttcttctc tttccagtca taggttgtgt caccacataa ccacggcaca ctgtctgatg
     3241 aaccaccaat acactgaccg gagaaagcac caagatccgc acggaatttt ttcaccatcg
     3301 cagtaaactg ggcaccatgc tgggccgggg tcgctttaag gtctccttcc ccctgaatcc
     3361 atacgacaga taacagcaca ttctttggat tcgcctcaag tgccgcttta gttcgactga
     3421 caagatcctg atacagtggg gtatccactc cccagcgtag tgacgcctcg gctgcaccac
     3481 ttgccggatc gaatgagcca actgcgccgg ttgtaaaagc agagccgcca cgggcacagg
     3541 gaaccagtaa aatcccggca tcagcaggaa tcaccggtaa tacttttttc gcaatatgaa
     3601 gtccctgccc cacacaacca tactgccctt ttttcaggtc tgcttttgga tggttcagca
     3661 gactcatatt ctgaacatca tgaaggcagt gatctgctgg aatgatttca ttgtatttgc
     3721 agggaatacc tccgggggtt tgtgtattcc tgcgtgccag ctgcataata cgtggatcag
     3781 gacgatcata agaatccgga aggggcaacc cttcaccata agatgaagca ttggactggc
     3841 cggctataac aatcacgtaa taccaggaag gggagctgga agccgtttca tttcctgccg
     3901 gacgggtatg tgtttctgca ttatcccagt ctgacggttc agtagtaccg gcaacatcag
     3961 cacttaacgg aatgacagca ccaatacccg ctccggtaat ttccacgttc ggatcaccgg
     4021 atactggcct tacccacagg caatcctgtg tattaaaact gatgagacag tcagcaaatg
     4081 tcattcctcc tttcgtttca gcaggaggaa agggagcctc aagaaatgcc gctgtaccac
     4141 cggaaacttt aacggtaaag tttccgggca ttgatgatag ttgaacccat ttggacgtca
     4201 taatattcat cctgttcctg aataaacaga attatcatat ccacagaccg ataaacgcca
     4261 ttttgcgtta attcagtaaa ggctcaacaa agggagcgtc ttatttttcc gaaacgctcg
     4321 caggataata acgataaagg gttgaaaggc cgatatcaaa aataagtgcc acctgtcttc
     4381 gactgacacc cccggccagt aaacgcccga cctgctcgta ttgttcctgc gtgagacgac
     4441 ggggtctgcc gccggttcgt cccctttcac gggcagctgc aagaccagcc cgtgtccgtt
     4501 cgacaattaa ttcacgctcc atttctgcca gcgcacccat tatgtgaaaa aagaagcgcc
     4561 ccatgggggt tgacgtatca atgttgtcag taagactacg aaaatttatc ccctgttgct
     4621 gtaattcatc tgtcagcgca acaaggtggc gcatactgcg ccccagcctg tccagtttcc
     4681 agaccatcag tgtgtcaccg ggttttagtt tcttcagtgc ccgcttcagt cccggccgct
     4741 gtgtcgtttt accactcatt ttgtcttcaa aaatctgctc acatcctgca cattccagcg
     4801 catttctttg taaaccggtg ttctggtcat ttgttgatac gcgtatatag ccaatcagca
     4861 tgtatttacc ttccttaaaa agaggtgaaa atgcctttta aaacagatat acggtatccc
     4921 tgaaaacctc ggtttacggg aaacggtaaa taaggcggcg aatgccctgc catcgaacgg
     4981 aacggctgtt gcagcgaata aactcgccac tccaagaaat attaatggtg tcccatttga
     5041 cggaacgcag gatataaata ttacatcggg gatgacgcag tctgtggctg actcacgtta
     5101 tgttcaaagc attcgaagag gttcaaggtc aacgattgga atgcaatata atatttttga
     5161 agttcctgac ggatgcgtac ttacagggct tgatgtcgct ggtgatggta atgcaacggt
     5221 aaccgcatat tatcgacctg ttcagtttct gatagatggt tcgtggaaaa cagcttccag
     5281 cgcataaaag tgcagtagtt ca
//
LOCUS       DAZFLD010000259         5291 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.261, whole genome shotgun sequence.
ACCESSION   DAZFLD010000259 DAZFLD010000000
VERSION     DAZFLD010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5291
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.261"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..488
                     /locus_tag="ACHKBL_004374"
     CDS             <1..488
                     /locus_tag="ACHKBL_004374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HFO5821145.1"
                     /translation="KGKVCHLLTSMTDAMRFPGGEMADLYSHRWEIELGYREIKQTMQ
                     LSRLTLRSKKPELVEQELWGVLLAYNLVRYQMIKMAEHLKGYWPNQLSFSESCGMVMR
                     MLMTLQGASPGRIPELMRDLASMGQLVKLPTRRERAFPRVVKERPWKYPTAPKKSQSV
                     A"
     gene            749..1090
                     /gene="raiA"
                     /locus_tag="ACHKBL_004375"
     CDS             749..1090
                     /gene="raiA"
                     /locus_tag="ACHKBL_004375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HFO5821146.1"
                     /translation="MTMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILSKE
                     PQGFVADATINTPNGVLVASGKHEDMYTAINELINKLERQLNKLQHKGEARRAATSVK
                     DANFVEEVEEE"
     misc_feature    1193..1318
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            1194..1241
                     /gene="pheL"
                     /locus_tag="ACHKBL_004376"
     CDS             1194..1241
                     /gene="pheL"
                     /locus_tag="ACHKBL_004376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pheA operon leader peptide PheL"
                     /protein_id="HFO5821147.1"
                     /translation="MKHTPFFFAFFFTFP"
     gene            1340..2500
                     /gene="pheA"
                     /locus_tag="ACHKBL_004377"
     CDS             1340..2500
                     /gene="pheA"
                     /locus_tag="ACHKBL_004377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HFO5821148.1"
                     /translation="MTSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHR
                     PVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPH
                     SARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIEN
                     TSSGAINDVYDLLQHTSLSIVGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSK
                     FLNRYPQWKIEYTESTSAAMEKVAQAKSPHVAALGSEAGGTLYGLQVLERIEANQRQN
                     FTRFVVLARKAINVSDQVPAKTTLLMATGQQAGALVEALLVLRNHNLIMTRLESRPIH
                     GNPWEEMFYLDIQANLESAEMQKALKELGDITRSMKVLGCYPSENVVPVDPT"
     gene            complement(2543..3664)
                     /gene="tyrA"
                     /locus_tag="ACHKBL_004378"
     CDS             complement(2543..3664)
                     /gene="tyrA"
                     /locus_tag="ACHKBL_004378"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HFO5821149.1"
                     /translation="MVAELTALRDQIDEVDKALLNLLAKRLELVAEVGEVKSRFGLPI
                     YVPEREASMLASRRAEAEALGVPPDLIEDVLRRVMRESYSSENDKGFKTLCPSLRPVV
                     IVGGGGQMGRLFEKMLTLSGYQVRILEQHDWDRAAEIVADAGMVIVSVPIHVTEQVIG
                     KLPPLPKDCILVDLASVKNGPLQAMLAAHDGPVLGLHPMFGPDSGSLAKQVVVWCDGR
                     KPEAYQWFLEQIQVWGARLHRISAVEHDQNMAFIQALRHFATFAYGLHLAEENVQLEQ
                     LLALSSPIYRLELAMVGRLFAQDPQLYADIIMSSERNLALIKRYYKRFGEAIELLEQG
                     DKQAFIDSFRKVEHWFGDYAQRFQSESRVLLRQANDNRH"
     gene            complement(3675..4745)
                     /gene="aroF"
                     /locus_tag="ACHKBL_004379"
     CDS             complement(3675..4745)
                     /gene="aroF"
                     /locus_tag="ACHKBL_004379"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroF"
                     /protein_id="HFO5821150.1"
                     /translation="MQKDALNNVHITDEQVLMTPEQLKAAFPLSLQQEAQIADSRKTI
                     SDIIAGRDPRLLVVCGPCSIHDPETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTT
                     VGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
                     SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHRFVGINQAGQ
                     VALLQTQGNPDGHVILRGGKAPNYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDY
                     RRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEMKYGVSVTDACISWE
                     MTDALLREIHQDLNGQLTARVA"
     gene            4955..>5291
                     /gene="yfiL"
                     /locus_tag="ACHKBL_004380"
     CDS             4955..>5291
                     /gene="yfiL"
                     /locus_tag="ACHKBL_004380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HFO5821151.1"
                     /translation="MMKKFIAPLLALLVSGCQIDPYTHAPTLTSTDWYDVGMEDAISG
                     NAIKDDDAFSDSQADRGLYLKGYAEGQKKTCQTDFTYARGLSGKSFPASCNNVENASQ
                     LHEVWQKGAD"
BASE COUNT         1448 a         1333 c         1243 g         1267 t
ORIGIN      
        1 gcaaaggaaa agtctgccat ctgctgacgt cgatgacgga cgccatgcgc ttccccggag
       61 gagaaatggc ggatctgtac agtcatcgct gggaaatcga actgggatac agggagataa
      121 aacagacgat gcaactgagc aggctgacgc tgagaagtaa aaagccggag cttgtggagc
      181 aagagctgtg gggtgtctta ctggcttata atctggtgag atatcagatg attaaaatgg
      241 cggaacatct gaaaggttac tggccgaatc aactgagttt ctcagaatca tgcggaatgg
      301 tgatgagaat gctgatgaca ttgcagggcg cttcaccggg acgtataccg gagctgatgc
      361 gcgatcttgc aagtatggga caacttgtga aattaccgac aagaagggaa agggccttcc
      421 cgagagtggt aaaggagagg ccctggaaat accccacagc cccgaaaaag agccagtcag
      481 ttgcttaact gatcggcatt acagcccttg agctgccgtt tttttattct gttagttgtg
      541 aaactgaagc gatttagtca ctatcgatct catcaaatat ggctcgcttt gagatattcc
      601 tcaagtaaaa aaacacctct tcctgcgatt tctcacaaaa aagattcgtt gacaaaaagt
      661 gacaaaatta tgagatttcc atcacacatt ttgacatcag gaacggtatg ctgaattcac
      721 caagacggga agacaagagg taaaatttat gacaatgaac attaccagca aacaaatgga
      781 aattactccg gccatccgcc aacatgtcgc agaccgtctc gccaaactgg aaaaatggca
      841 aacacatctg attaatccac atatcattct gtccaaagag ccacaagggt ttgttgctga
      901 cgccacaatc aatacaccta acggcgttct ggttgccagt ggtaaacatg aagatatgta
      961 caccgcaatt aacgaattga tcaacaagct ggaacggcag ctcaataaac tgcagcacaa
     1021 aggcgaagca cgtcgtgccg caacatcggt gaaagacgcc aacttcgtcg aagaagttga
     1081 agaagagtag tcctttatat tgagtgtatc gccaacgcgc cttcgggcgc gttttttgtt
     1141 gacagcgtga aaacagtacg ggtactgtac taaagtcact taaggaaaca aacatgaaac
     1201 acacaccgtt tttcttcgca ttctttttta ccttcccctg aatgggaggc gtttcgtcgt
     1261 gtgaaacaga atgcgaagac gaacaataag gcctcccaaa tcggggggcc ttttttattg
     1321 ataacaaaaa ggcaacacta tgacatcgga aaacccgtta ctggcgctgc gagagaaaat
     1381 cagcgcgctg gatgaaaaat tattagcatt actggcagag cgacgcgaac tggccgtcga
     1441 ggtgggaaaa gccaaactgc tctcgcatcg cccggtacgt gatattgatc gtgaacgcga
     1501 tttgctggaa agattaatta cgctcggtaa agcgcaccat ctggacgccc attacattac
     1561 tcgcctgttc cagctcatca ttgaagattc cgtattaact cagcaggctt tgctccagca
     1621 acatctcaat aaaattaatc cgcactcagc acgcatcgct tttctcggcc ccaaaggttc
     1681 ttattcccat cttgcggcgc gccagtatgc tgcccgtcac tttgagcaat tcattgaaag
     1741 tggctgcgcc aaatttgccg atatttttaa tcaggtggaa accggccagg ccgactatgc
     1801 cgtcgtaccg attgaaaata ccagctccgg tgccataaat gacgtttacg atctgctgca
     1861 acataccagt ttgtcgattg ttggcgagat gacgttaact atcgaccatt gtttgttggt
     1921 ctccggcact actgatttat ccaccatcaa tacggtctac agccatccgc agccattcca
     1981 gcaatgcagc aaattcctta atcgttatcc gcaatggaag attgaatata ccgaaagtac
     2041 gtctgcggca atggaaaagg ttgcacaggc aaaatcaccg catgttgctg cgttgggaag
     2101 cgaagctggc ggcactttgt acggtttgca ggtactggag cgtattgaag cgaatcagcg
     2161 acaaaacttc acccgatttg tggtgttggc acgtaaagcc attaacgtgt ctgaccaggt
     2221 tccagcgaaa acgacgttgt taatggcgac cgggcaacaa gctggtgcgc tggttgaagc
     2281 gttgctggta ctgcgcaacc acaatctgat tatgacccgt ctggagtcac gcccgattca
     2341 cggtaatcca tgggaagaga tgttttatct ggatattcag gccaatcttg aatcagcgga
     2401 aatgcaaaaa gcattgaaag agttagggga catcacccgt tcgatgaagg tattgggctg
     2461 ttacccaagt gagaacgtag tgcctgttga tccaacctga tgaaaaggtg ccggatgatg
     2521 tgaatcaccc ggcactggat tattagtgac ggttgtcatt cgcctgacgc aataacacgc
     2581 ggctttcact ctgaaaacgc tgtgcgtaat cgccgaacca gtgctccacc ttgcggaaac
     2641 tgtcaataaa cgcctgctta tcgccctgct ccagcaactc aatcgcctcg ccgaaacgct
     2701 tatagtaacg tttgattaac gccagattac gctctgacga cataataatg tcggcataca
     2761 gttgcggatc ctgggcaaac agtcgcccaa ccatcgccag ctcaaggcgg taaatcggcg
     2821 aagaaagcgc cagaagttgc tcaagctgaa cattttcttc tgccaggtgc agcccataag
     2881 caaaagtagc aaagtggcgc agtgcctgaa taaacgccat attctgatcg tgctcgacag
     2941 cgctaatacg atgcagccga gcgccccaga cctgaatttg ctccagaaac cattggtatg
     3001 cttccggctt acgtccatca caccagacca caacttgttt tgccaggcta ccgctgtccg
     3061 ggccgaacat cgggtgtaac cccagcaccg gaccatcatg cgccgccagc atggcctgta
     3121 atggtccatt tttcaccgat gccagatcga ccagaataca atctttcggt aaaggcggta
     3181 atttgccaat aacttgttca gtaacgtgga tgggcacact aacaatcacc attccggcat
     3241 cggcaactat ctcagccgct cgatcccagt catgttgctc cagaatccgc acctgataac
     3301 ccgatagtgt cagcatcttc tcgaacaggc gtcccatctg accgccaccg ccgacgataa
     3361 ccaccgggcg cagtgacgga caaagcgttt taaatccttt gtcgttttca ctggagtaag
     3421 attcacgcat cacccgacgc aaaacatcct caatcagatc tggcggtaca cccagagctt
     3481 ccgcctctgc acgacgcgag gccaacatag atgcctcgcg ctccggaaca taaataggca
     3541 gtccaaagcg gcttttcacc tcgcccactt cagcaaccag ttccagacgc ttcgctaata
     3601 aattcagcag cgctttatcg acttcatcaa tttgatcgcg taatgcggtc aattcagcaa
     3661 ccataataaa cctcttaagc cacgcgagcc gtcagctgcc cgttcagatc ctgatgaatt
     3721 tcacgcagca aggcatcggt catttcccag ctaatgcagg catcggttac ggatacaccg
     3781 tatttcattt cactgcgcgg ttgctcggaa gactgattgc cctcgtggat attactttcg
     3841 atcatcagac caataattga gcgattgcca tctttgattt gagcaaccac ggattctgcc
     3901 accgcaggct gacggcgata atctttattg gaattaccgt ggctgcaatc taccatcaga
     3961 gacgggcgca gtcccgcctg ttccatctct ttttcacatt gcgcaacatc cgcagggcta
     4021 tagttcggcg ctttaccacc gcgcaggatc acatgcccgt ccggattccc ctgagtttgt
     4081 agcaacgcca cctgccctgc ctggttaatg ccaacaaaac ggtgcggctg ggcggcggca
     4141 cgcatagcgt taatagccgt tgcaaggctg ccgtcggtgc cgtttttaaa accaaccggc
     4201 atggaaagcc cggaggccat ttcacggtgt gtttgcgatt ccgttgtacg agcaccaatt
     4261 gctgaccagc taaacagatc gcccaggtat tgcgggctat tcggatctaa cgcttccgtc
     4321 gccagtggca gtcccatatt caccagctca agcagcaatt tacgcgcgat ctgcagcccg
     4381 gcttctacat caaaagagcc atccatatgg ggatcgttaa tcaacccttt ccaaccgaca
     4441 gtggtacggg gtttttcaaa atagacgcgc attaccagat agaggctatc gctgacctct
     4501 gcggcaaggg ctttaaatcg acgagcatat tccagagcag tttccggatc atgaatggaa
     4561 caaggaccac atactaccaa cagacgagga tcgcgcccgg cgataatatc tgaaatggtt
     4621 ttacgcgagt cagcaatctg ggcttcctgt tgcaggctta atggaaaagc ggccttcagt
     4681 tgttccggag tcattaaaac ttgttcgtcg gtaatatgta cgttattcag cgcgtctttt
     4741 tgcatgatgg cgatcctgtt tatgctcgtt tgcgatagtt gatcctcagc gaggatgacg
     4801 taacgataac acataaagta aagttttcaa tccatatttc gtacattttt ctttacacaa
     4861 gcaatttatt cgcgctttta acccttgttt atgtataaat aaatttacac cacctttgaa
     4921 aacaatccgc tatgctttga aaaaggagaa agaaatgatg aaaaagttta tcgccccctt
     4981 gttggcttta ctggttagcg gatgtcagat tgatccttat actcacgcgc caaccttgac
     5041 cagcaccgac tggtatgatg ttggtatgga agatgcgata tcgggcaacg ccataaaaga
     5101 tgacgatgca tttagcgatt cacaggcgga tcgcggtcta taccttaaag gatatgccga
     5161 aggacaaaag aaaacttgcc agaccgattt tacttatgcc cgaggacttt ccggtaaaag
     5221 ctttcctgcc agctgcaaca atgttgaaaa tgccagccaa ctgcatgaag tatggcaaaa
     5281 aggggctgat g
//
LOCUS       DAZFLD010000260         5229 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.262, whole genome shotgun sequence.
ACCESSION   DAZFLD010000260 DAZFLD010000000
VERSION     DAZFLD010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5229)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5229)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5229
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.262"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..919
                     /locus_tag="ACHKBL_004381"
     CDS             <1..919
                     /locus_tag="ACHKBL_004381"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFO5821152.1"
                     /translation="CDNMPENGHVMRDVVTSYAQAVDVKLAQWIEENVTFPSTMVDRI
                     VPAVTEDTLAKIEQLTGVRDPAGVACEPFRQWVIEDNFVAGRPEWEKAGAELVSDVLP
                     YEEMKLRMLNGSHSFLAYLGYLAGYQHINDCMEDEHYRHAAYALMLQEQAPTLKVQGV
                     DLQDYANRLIERYSNPALRHRTWQIAMDGSQKLPQRMLDSVRWHLAHDSKFDLLALGV
                     AGWMRYVGGVDEQGNPIEISDPLLPVIQKAVQSSAEGKARVQSLLAIKAIFGDDLPDN
                     SLFTAKVTEAYLSLLAHGAKATVAKYSVK"
     gene            complement(955..1158)
                     /gene="ydfZ"
                     /locus_tag="ACHKBL_004382"
     CDS             complement(955..1158)
                     /gene="ydfZ"
                     /locus_tag="ACHKBL_004382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium delivery protein YdfZ"
                     /protein_id="HFO5821153.1"
                     /translation="MTTYDRNRNAITTGSRVMVSGTGHTGKILSIDTEGLTAEQIRRG
                     KTVVVEGCEEKLAPLDLIRLGMN"
     gene            complement(1335..2021)
                     /gene="rspR"
                     /locus_tag="ACHKBL_004383"
                     /gene_synonym="ydfH"
     CDS             complement(1335..2021)
                     /gene="rspR"
                     /locus_tag="ACHKBL_004383"
                     /gene_synonym="ydfH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator rspR"
                     /protein_id="HFO5821154.1"
                     /translation="MTVETQLNPTQPVNQQIYRILRRDIVHCLIAPGTPLSEKEVSVR
                     FNVSRQPVREAFIKLAENGLIQIRPQRGSYVNKISMAQVRNGSFIRQAIECAVARRAA
                     SMITESQCYQLEQNLHQQRIAIERKQLDDFFELDDNFHQLLTQIADCQLAWDTIENLK
                     ATVDRVRYMSFDHVSPPEMLLRQHLEIFSALQKRDGDAVERAMTHHLQEISESVRQIR
                     QENSDWFSEE"
     gene            complement(2110..2856)
                     /gene="ydfG"
                     /locus_tag="ACHKBL_004384"
     CDS             complement(2110..2856)
                     /gene="ydfG"
                     /locus_tag="ACHKBL_004384"
                     /EC_number="1.1.1.298"
                     /EC_number="1.1.1.381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent 3-hydroxy acid
                     dehydrogenase/3-hydroxypropionate dehydrogenase YdfG"
                     /protein_id="HFO5821155.1"
                     /translation="MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELKDEL
                     GDNLYIAQLDVRNRAAIEEMLASLPAEWCNIDILVNNAGLALGMEPAHKASVEDWETM
                     IDTNNKGLVYMTRAVLPGMVERNHGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLN
                     LRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDSKAEKTYQNTVALTPEDVSEAVWW
                     VSTLPAHVNINTLEMMPVTQSYAGLNVHRQ"
     gene            2993..5038
                     /gene="dcp"
                     /locus_tag="ACHKBL_004385"
     CDS             2993..5038
                     /gene="dcp"
                     /locus_tag="ACHKBL_004385"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HFO5821156.1"
                     /translation="MTTMNPFLVQSTLPYLAPHFDQIANHHYRPAFDEGMQQKRAEIA
                     AIALNPQAPDFNNTILALEQSGELLTRVTSVFFAMTAAHTNDELQRLDEQFSAELAEL
                     ANDIYLNGELFARVDAVWQRRESLGLDSESIRLVEVIHQRFVLAGAKLAQADKAKLKV
                     LNTEAATLTSQFNQRLLAANKSGGLVVNDFAQLAGMSEQEIALAAEAAREKGLDNKWL
                     IPLLNTTQQPALAELRDRATREKLFTAGWTRAEKNDANDTRAIIQRLVEIRAQQAKLL
                     GFPHYAAWKIADQMAKTPEAALNFMREIVPAARQRASDELVSIQAVIDKQQGGFSAQP
                     WDWAFYAEQVRREKFDLDESQLKPYFELNTVLNEGVFWTANQLFGIKFVERFDIPVYH
                     PDVRVWEIFDHNGVGLALFYGDFFARDSKSGGAWMGNFVEQSTLNETHPVIYNVCNYQ
                     KSAAGEPALLLWDDVITLFHEFGHTLHGLFARQRYATLSGTNTPRDFVEFPSQINEHW
                     ATHPQVFARYARHYQSGAAMPDELQQKMRNASLFNKGYEMSELLSAALLDMRWHCLEE
                     NEAMQDVDDFELRALVAENMDLPAIPPRYRSSYFAHIFGGGYAAGYYAYLWTQMLADD
                     GYQWFVEQGGLTRENGRRFREAILSRGNSEDLERLYRQWRGKAPQIMPMLQHRGLNV"
     gene            complement(5083..5163)
                     /locus_tag="ACHKBL_004386"
                     /pseudo
     CDS             complement(5083..5163)
                     /locus_tag="ACHKBL_004386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310173.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1298 a         1247 c         1354 g         1330 t
ORIGIN      
        1 atgtgacaac atgccagaaa acggtcatgt tatgcgtgac gttgtcactt cctacgcgca
       61 agctgttgat gtaaaactgg cacaatggat cgaagaaaac gtgactttcc catcaacaat
      121 ggtggaccgt attgtgcccg cagtgacgga ggatacgctg gcgaaaatcg aacaacttac
      181 cggtgtgcgc gatcctgcgg gcgttgcctg tgaacctttc cgccagtggg taatagaaga
      241 taactttgtt gccggacgtc cggaatggga aaaagcggga gccgaactgg ttagtgatgt
      301 gctgccttat gaagagatga agttgcgcat gctcaacggc agtcattcat tcctggcgta
      361 tctggggtat cttgccggat atcagcacat taatgactgc atggaagatg aacattatcg
      421 tcatgcagcg tatgccttga tgttgcagga acaagcgccg acgttgaaag tgcagggcgt
      481 tgatttgcaa gattacgcta accgattaat tgaacgctat agcaacccgg cgctacgtca
      541 tcgaacctgg cagattgcga tggatggtag ccagaaattg ccacagcgga tgttggattc
      601 tgttcgctgg catctggcgc atgacagcaa gttcgatctg ctggcgctgg gcgtcgcagg
      661 ttggatgcgt tatgtcggtg gtgttgatga acagggaaat ccgatagaaa tcagtgaccc
      721 actgttacct gttattcaga aggctgtaca aagtagtgcc gaagggaaag cgcgcgtcca
      781 gtcattgctg gcgattaagg cgatctttgg tgatgatttg ccagacaata gcttgtttac
      841 tgcaaaagtg acggaagcgt acttgtcttt attagcgcat ggtgcgaaag cgaccgtggc
      901 gaaatattcc gtgaagtaaa aaaagcaatg ccgccatcgg cggcattcac acgcttagtt
      961 catgccgaga cgaatcaggt ccagaggtgc cagtttctct tcacaacctt caacaactac
     1021 ggtttttccg cggcggattt gctcagcggt cagaccttca gtatcaatcg acaggatctt
     1081 gccagtgtga ccggtgccgc taaccataac acggctgcca gtggtgattg cgttacggtt
     1141 acgatcgtag gtcgtcatag gtgttttctc ctttctgatt tacagtcgaa gcccacgtaa
     1201 cgggcgcact ataaatactc ctgtcgagtg aaatattttt tgtttttgat caagctcaca
     1261 tcttttttga tgcatgcatg agccatatga cggagggaaa aactctcacc ccggatggga
     1321 tgagaggaaa tgaattactc ttcgctaaac cagtcgctgt tttcctgacg gatctggcgc
     1381 acggattcgc tgatttcctg caaatggtgc gtcattgcac gttctaccgc atcgccatca
     1441 cgtttttgca gggcagagaa aatctcaaga tgctggcgta acagcatttc tggtggagaa
     1501 acgtggtcga aactcatata gcgcacgcga tcaacggtcg ctttcaggtt ctcaatggta
     1561 tcccacgcca gttgacagtc ggcaatctgc gtcaggagtt gatggaagtt gtcatcaagt
     1621 tcaaaaaaat catccagttg cttgcgctct atggcaatgc gttgctggtg aagattttgt
     1681 tccagttggt agcactggct ttcggtaatc atgctcgccg cccgacgcgc caccgcgcac
     1741 tcaatggcct gacggataaa actgccgttg cgcacctggg ccatggaaat tttgttgacg
     1801 tagctgccac gctgcggacg aatttgaatc aggccgtttt ccgccagttt aataaaggct
     1861 tcacgaaccg gctggcgtga cacattgaaa cgaacagaaa cttctttttc cgacaacggt
     1921 gtgcctggtg caatcaggca atggacaatg tcgcgacgaa gaatacgata aatctgctga
     1981 ttgacaggct gtgtgggatt aagttgcgtt tcgacggtca ttcgttcttg cttgtgagtg
     2041 agttaactgc atgagtctac cacttttttg tgacaagcaa taacccggca gttacgccgg
     2101 gtataaaaat tactgacggt ggacattcag tccggcatag ctttgggtaa ccggcatcat
     2161 ttccagggta ttgatattga cgtgagcagg cagcgttgac acccaccaga cggcttcgct
     2221 gacatcttcc ggcgtcaatg caacggtatt ttgataggtt ttttccgctt tactgtcatc
     2281 gcctttaaag cggacattgg aaaactcggt gccacccacc agacccggtt cgatgtcggt
     2341 gacgcgcacc gccgtaccat gcaggtccgt acgcagattc aggctaaact gacgaacaaa
     2401 cgctttcgtc gcaccgtaaa cgttaccacc ggcatacggc cagctgcctg ccgttgagcc
     2461 aatgttaata atatgaccat gattacgttc aaccataccc ggtaagacgg cgcgcgtcat
     2521 atataccagg cctttgttgt tggtatcaat catcgtttcc cagtcttcaa cgctggcttt
     2581 atgcgcaggc tccatgccca acgccaggcc ggcattattt accaggatat caatattgca
     2641 ccactcggca ggaagcgatg ccagcatctc ttcaatagcg gcgcggttgc gaacgtccag
     2701 ttgggcgata tacagattat ctcccagttc gtcttttaac tcctgcaacc gctcctggcg
     2761 acggccagtg gcgataactt tatgcccttg ttgaataaaa cgacgagtaa tgcattcacc
     2821 aaaacctgcc gttgctccag ttactaaaac gatcatctca ctgttcctca acgcttttgt
     2881 gtgtcaataa catagcacgt gatgcttaag agcgggtaat actggtttgc aagattgcac
     2941 tccgttatca gtaagcctac gctatggaac acctcgcgtt caggagaaga aaatgacaac
     3001 aatgaatcct ttccttgtgc aaagcacact gccgtatctg gctccccatt ttgatcaaat
     3061 tgccaatcat cactatcgcc cggcattcga tgagggaatg cagcaaaagc gggcagaaat
     3121 tgctgccatc gcgcttaacc cgcaagcgcc tgatttcaac aatactattc tggcactgga
     3181 acaaagcgga gaattactta cccgcgttac cagcgtcttt tttgcgatga ctgcggcgca
     3241 tactaatgat gaattacagc gtcttgatga acagttttcc gctgaactgg cggaactggc
     3301 taatgatatc tatctgaacg gtgaattatt cgcgcgggta gatgctgtct ggcaacgccg
     3361 tgaatccctg ggacttgata gtgaatccat ccgcctggtg gaggtcattc atcaacgttt
     3421 tgtccttgcc ggagccaaac ttgcacaagc tgataaagca aaattaaaag tactgaatac
     3481 agaagctgcg accctgacca gccagtttaa ccagcggtta ctggcagcaa ataaatccgg
     3541 cggtctggtt gtgaacgatt tcgcgcagct ggcaggaatg agtgagcaag agattgcgct
     3601 ggcggcagag gcggctcgcg agaaaggtct ggataacaaa tggctgattc cgttgctgaa
     3661 taccacccag caaccagcgc ttgctgaact gcgcgatcgc gcgacgcgtg aaaaactgtt
     3721 cactgcgggc tggacgcgag cggaaaaaaa tgatgccaat gatacccgcg ctatcattca
     3781 acgtctggtg gagatccgcg cgcagcaggc gaaactgctt ggtttccctc attatgccgc
     3841 atggaaaatc gccgatcaga tggcaaaaac gccagaagca gcactcaact ttatgcggga
     3901 aattgttcca gcggcgcgtc aacgtgcgag cgatgaatta gtctccatac aggcggttat
     3961 tgataagcag cagggcgggt ttagcgcgca gccgtgggac tgggcatttt atgccgagca
     4021 ggtaagacgt gagaaattcg atctcgatga atcgcagctc aagccatatt ttgaattaaa
     4081 cacggtgttg aatgaaggtg tattctggac cgcgaatcag ctcttcggta ttaagtttgt
     4141 cgaacgtttt gatattcctg tctaccatcc tgacgttcgt gtgtgggaaa tttttgatca
     4201 taatggcgtg gggctggcgt tattttacgg tgatttcttc gcccgtgatt caaaaagcgg
     4261 cggtgcatgg atgggcaatt ttgttgagca atcaacgctg aatgaaacgc atccggtaat
     4321 ttataacgtc tgcaattatc agaaatccgc cgctggggag cctgcgttgt tactctggga
     4381 tgatgtcata accttattcc atgaatttgg tcatacgctg cacggccttt ttgcccgcca
     4441 gcgttatgcc acgctttccg gcaccaacac gccgcgtgat tttgtcgaat ttccgtcgca
     4501 aatcaacgaa cactgggcaa cgcatccgca ggtattcgct cgctacgccc ggcattatca
     4561 gagcggggca gcaatgcctg atgaactgca acagaaaatg cgtaatgcca gcctgttcaa
     4621 taaagggtat gagatgagcg aactgcttag cgccgcactt ctcgatatgc gctggcattg
     4681 cctggaagaa aacgaagcaa tgcaggatgt cgatgatttt gaattgcggg cgctggtggc
     4741 ggaaaatatg gatcttcctg ctataccgcc acgctatcgc agcagttatt tcgcccatat
     4801 ttttggtggc ggatatgccg caggttatta cgcttatctg tggacgcaaa tgttggccga
     4861 tgatggttac cagtggtttg ttgagcaggg cggattaacg cgtgaaaatg ggcggcgttt
     4921 tcgcgaggcg attctttcca gaggtaacag cgaagatctg gaacgcctgt atcgacaatg
     4981 gcgcggtaag gcacctcaga ttatgccgat gctgcaacat cgtggcttga acgtataaac
     5041 accaccaatg gctggcctgg atttatcgcc agaccagcct cgttatagca gtaattgcag
     5101 ccgctgggtg agggcaaact tcaccgctaa tgcgaatgcc gtttcgcagt cgccagcaaa
     5161 attacttttt ttgtataccc ttccgttata ataattttca aatttataaa tctgattgtt
     5221 ctgaaatat
//
LOCUS       DAZFLD010000261         5213 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.263, whole genome shotgun sequence.
ACCESSION   DAZFLD010000261 DAZFLD010000000
VERSION     DAZFLD010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5213
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.263"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            74..189
                     /gene="rrf"
                     /locus_tag="ACHKBL_004387"
     rRNA            74..189
                     /gene="rrf"
                     /locus_tag="ACHKBL_004387"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(252..1010)
                     /gene="yhdZ"
                     /locus_tag="ACHKBL_004388"
     CDS             complement(252..1010)
                     /gene="yhdZ"
                     /locus_tag="ACHKBL_004388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFO5821157.1"
                     /translation="MSQILLQPANAMITLENVNKWYGQFHVLKNINLTVQPGERIVLC
                     GPSGSGKSTTIRCINHLEEHQQGRIVVDGIELNEDIRNIERVRQEVGMVFQHFNLFPH
                     LTVLQNCTLAPIWVRKMPKKEAEALAMHYLERVRIAEHAHKFPGQISGGQQQRVAIAR
                     SLCMKPKIMLFDEPTSALDPEMVKEVLDTMIGLAQSGMTMLCVTHEMGFARTVADRVI
                     FMDRGEIVEQAAPDEFFAHPKSERTRAFLSQVIH"
     gene            complement(1018..2121)
                     /gene="yhdY"
                     /locus_tag="ACHKBL_004389"
     CDS             complement(1018..2121)
                     /gene="yhdY"
                     /locus_tag="ACHKBL_004389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFO5821158.1"
                     /translation="MTKVLLSHPPRPASHNSSRAMVWVRKNLFSSWSNSLLTIGCIWL
                     MWELIPPLLNWAFLQANWVGSTRTDCTKAGACWVFIHERFGQFMYGLYPHDQRWRINL
                     ALLIGLVSIAPMFWKILPHRGRYIAVWAVIYPLIVWWLMYGGFLGLERVETRQWGGLT
                     LTLIIASVGIAGALPWGILLALGRRSHMPIVRILSVIFIEFWRGVPLITVLFMSSVML
                     PLFMAEGTSIDKLIRALVGVILFQSAYVAEVVRGGLQALPKGQYEAAESLALGYWKNQ
                     GLVILPQALKLVIPGLVNTIIALFKDTSLVIIIGLFDLFSSVQQATVDPAWLGMSTEG
                     YVFAALIYWIFCFSMSRYSQHLEKRFNTGRTPH"
     gene            complement(2131..3312)
                     /gene="yhdX"
                     /locus_tag="ACHKBL_004390"
                     /pseudo
     CDS             complement(2131..3312)
                     /gene="yhdX"
                     /locus_tag="ACHKBL_004390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026209.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
     gene            complement(3380..4405)
                     /locus_tag="ACHKBL_004391"
     CDS             complement(3380..4405)
                     /locus_tag="ACHKBL_004391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO5821159.1"
                     /translation="MKKMMIATLAAASVLLAVANQAHAGATLDAVQKKGFVQCGISDG
                     LPGFSYADADGKFSGIDVDVCRGVAAAVFGDDTKVKYTPLTAKERFTALQSGEVDLLS
                     RNTTWTSSRDAGMGMAFTGVTYYDGIGFLTHDKAGLKSAKELDGATVCIQAGTDTELN
                     VADYFKANNMKYTPVTFDRSDESAKALESGRCDTLASDQSQLYALRIKLSNPAEWIVL
                     PEVISKEPLGPVVRRGDDEWFSIVRWTLFAMLNAEEMGINSQNVDEKAANPATPDMAH
                     LLGKEGDYGKDLKLDNKWAFNIIKQVGNYSEIFERNVGSESPLKIKRGQNNLWNNGGI
                     QYAPPVR"
     gene            complement(4836..5057)
                     /locus_tag="ACHKBL_004392"
     CDS             complement(4836..5057)
                     /locus_tag="ACHKBL_004392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5821160.1"
                     /translation="MKRLIPVALLTALLAGCAHDSPCVPVYDDQGRLVHTNTCMKGTT
                     QDNWETAGAIAGGAAAVAGLTMGIIALSK"
BASE COUNT         1453 a         1336 c         1224 g         1200 t
ORIGIN      
        1 gagagaagat tttcagcctg atacagatta aatcgacagg tcataacgag acgtgttgat
       61 aaaacagaat ttgcctggcg gccttagcgc ggtggtccca cctgacccca tgccgaactc
      121 agaagtgaaa cgccgtagcg ccgatggtag tgtggggtct ccccatgcga gagtagggaa
      181 ctgccagaca tcaaatcaag cgaaaggcca tccgaaagga tggccttttt gcttttcgaa
      241 ctaacattca attaatggat cacctgcgat aaaaatgccc tcgtacgctc tgatttagga
      301 tgcgcaaaaa actcatccgg tgcagcttgt tccactatct ccccacgatc cataaaaatc
      361 accctgtcag cgacggttcg tgcaaacccc atctcatgtg ttacacacag cattgtcata
      421 cccgactgcg ccagcccaat catcgtatcc agcacctctt tcaccatctc aggatcgagc
      481 gccgacgttg gctcatcaaa taacataatt ttcggcttca tacacagcga acgcgcaatg
      541 gcaacgcgtt gctgctgacc acctgaaatc tgtccgggaa acttatgcgc atgttcggca
      601 attctcaccc gctcaaggta atgcatcgcc agagcttctg cctctttctt tggcatcttg
      661 cgtacccaaa tcggtgccag ggtacagttc tgtagaacgg tcagatgagg gaagagattg
      721 aaatgctgaa agaccattcc cacttcctgc ctgacgcgct caatattgcg aatatcttcg
      781 ttaagctcga tgccatctac cacgatccgt ccctgttggt gttcttccag atgattaata
      841 caacgaatgg ttgtcgattt accggaacca gaagggccac acagaacgat ccgttctccc
      901 ggttgcacgg ttaaatttat atttttcaaa acatggaatt gcccatacca tttattgacg
      961 ttttccagcg taatcatcgc gttagcaggt tgcagtaaaa tttggctcat agtgtcctca
     1021 atgcggtgta cgcccggtgt taaaacgttt ttccagatgt tggctataac gtgacatgct
     1081 gaaacagaag atccagtaga tcagtgcggc gaaaacatac ccttccgtcg acatacccaa
     1141 ccaggcggga tcaacggttg cctgctgaac gctactgaaa agatcgaaca acccgatgat
     1201 gatcaccagg ctggtatctt tgaagagtgc gatgatggta tttaccagcc caggaatcac
     1261 cagcttcaac gcctgtggca gaataaccag cccctggttt ttccagtaac ccaacgccag
     1321 cgactctgcc gcttcatatt gccctttagg cagcgcctgt aatccgcctc gcacgacttc
     1381 cgcaacatat gctgactgaa acaggatcac gccaaccagc gcgcggatca atttgtcgat
     1441 actggtgcct tctgccataa acaacggcag catgaccgaa gacataaaca gaacggttat
     1501 cagcggtaca ccgcgccaga actcgataaa aatgaccgat aagatacgca caatcggcat
     1561 atgggagcgg cgacctaacg ccagtaagat cccccacggc agcgcccccg caatcccaac
     1621 tgatgcaata attaaagtta gcgtcagccc gccccattgc cgggtttcaa cccgctcaag
     1681 accaagaaac ccgccataca tcagccacca gacaatcagt gggtaaatca ccgcccatac
     1741 cgcaatatag cgaccgcgat gcgggagtat tttccagaac attggtgcga tcgacacaag
     1801 cccaatcagt aatgcgaggt taattcgcca gcgttggtcg tgtgggtaaa gcccatacat
     1861 aaactgacca aatcgttcgt ggatgaagac ccaacaggca ccggcttttg tgcagtcggt
     1921 acgcgttgag ccaacccagt tagcctgcaa aaatgcccag ttcagcaacg gtggaatcag
     1981 ttcccacatc aaccatatgc agccaatagt cagcaggcta ttgctccagc tggagaacag
     2041 attttttcgc acccacacca tcgcgcggct tgagttatgg ctcgccgggc gcgggggatg
     2101 agacagcaat acttttgtca tcatgactcc ttagcgttca acgatcgcga tgcggcggtt
     2161 atagatattc atcagcagcg agatagtcag gctgataatc agatagaccg acatggtcat
     2221 ggctatcgtc tcgatggctt gccccgtctg gttcagcacg gtgccagcaa acagcgaaac
     2281 catatccgga tagccaatag cggcggcaag agaggagttt ttgacgatgt tgagatactg
     2341 gctggttaac ggcggaataa tcactcgcag tgcctgggga ataatgacct ggcgtagcgt
     2401 aaccggattg ggtaatccca gtgaccgagc cgcttcatgt tgaccataag gcactgcctg
     2461 gatcccagcg cggataatct cggcgataaa tgcagaggta taaaccgaaa gcgccagcgt
     2521 taaggctgcc agttcaggaa ttaaaaccat cccgccacgg aagttaaagc ctcgtagggc
     2581 tggaacatcc cagtgtagtg ctgcgccaaa aagccattgc gccagtaaag gcaaacctat
     2641 gatcaacata acggcgaccg gccaggttcg gcgcaattgt ccggtcttta tctggtacgt
     2701 tttattaaaa cggaatagtc caacagaaat ggctatagca ataacaacag tcacgataaa
     2761 cgcaataaat ccgtctccca gctgcggaga tggaatataa agcccacgat tgctcaaaaa
     2821 ggccagatca aacgcactca ctgcctggcg tggtccgggc aaattgcgca acacggcaaa
     2881 gtatcagaag aagatttgca gcagcggcgg aatattacgg aagatctcaa tataaattgt
     2941 ggaaagcttt cgtagcagcc agttatccga aagtcgcgcc agaccgataa agaagcccag
     3001 aacagaagcg aatacaatac acaatgcaga aaccagtagc gtattgagta agccaacaat
     3061 gaaaacgcgt ccgtaggtat caccctgctg gtaatcgatc aaatgctgga caataccgaa
     3121 gccagcgccg cgatccagaa aggcaaaacc tgaagtaatg ccacgattac tgagattcgt
     3181 cacagtgttg tgaaatagcc aaccaacaat tccgacaaca gcaacaacgg caaagatctg
     3241 gaataaccag gcgcgaaccg tagggttggc aaaggagagt gagcctttaa cggttgagcg
     3301 gcgatgagac atagaaacct cggaaagcat aacgctgaag agagcgccgc cgtagcgacg
     3361 ctcgttacct cacaccgact taacgcacgg gcggcgcgta ctgaataccg ccgttattcc
     3421 agagattatt ttgcccacgt ttaattttca gcgggctttc tgaacctacg ttacgctcga
     3481 aaatttccga gtagttaccc acctgtttga tgatgttaaa ggcccattta ttatccagct
     3541 tcagatcctt gccgtaatcg ccttctttac ccagcagatg tgccatatca ggtgttgctg
     3601 gattagccgc tttttcatcg acgttctggg aattgatgcc catctcttca gcattcagca
     3661 tggcgaaaag cgtccagcgt acaatcgaga accattcatc atcgccacga cgaactaccg
     3721 gcccaagtgg ttctttagag ataacttccg gtaagacaat ccattcagcc gggttgctta
     3781 atttgatgcg cagggcatac agttgtgatt gatccgaggc cagcgtatcg cagcgaccag
     3841 attccagtgc cttcgctgat tcgtcagagc gatcgaaagt cactggtgtg tacttcatat
     3901 tgtttgcctt aaagtaatcg gcaacgttga gttcggtatc agtacccgcc tgaatacaga
     3961 cggtagcgcc atccagttct ttcgcgcttt ttagccccgc tttatcgtgc gtcaggaagc
     4021 caatgccgtc gtaataagtg acgccagtaa atgccattcc catcccggca tcgcgagatg
     4081 aagtccaggt cgtattacgg gagagcaaat ccacctcccc tgactgtaaa gcggtgaagc
     4141 gttcttttgc agtgagcggg gtatatttca ctttcgtatc gtcaccgaat acagcagcgg
     4201 caacaccacg acaaacatct acatcaatac ctgaaaactt accgtcagca tcggcataag
     4261 agaacccagg taatccatca ctgatcccgc attgcacaaa accttttttc tgaacggcat
     4321 caagcgtcgc gccagcatgc gcctgatttg caacggcaag cagcacgctg gcggcagcca
     4381 gtgtggctat catcatcttt ttcataatgc ttcctgtgag gcgaaaatta tcgttatagg
     4441 acgtttggga aacgtctttt acctgtctgt ggccatcctc ttataagcaa aagtagtgcc
     4501 agcttttgcg cacgatttcg cgtcgcaata agctgagtgt agacaggaaa taataaaaac
     4561 tgcgccccgc aatggtgcga aaatgaaaca tgcgccacat tttggcgcaa aatatttacg
     4621 cgttttttac taagcagaat aatgattgtt agcgtgagta tatttctggt gggatgtgtg
     4681 aaagttaaat tgatgcgtta tttcgaaagc agtgaaataa cgtgatataa catgctaaat
     4741 cagcagggaa ttacccagca gaatatagca atattttatc ttatggaaag atactgtatc
     4801 gttaaaaaga gcgcggagct ccgcgccctg tttagttatt tcgacagagc gatgatcccc
     4861 atcgtcaggc cagcaacagc tgctgccccg ccggcaatag ccccagccgt ttcccagtta
     4921 tcctgagtcg tgcctttcat acaggtattg gtgtgaacca gacgaccctg atcgtcataa
     4981 accggaacgc agggcgaatc gtgcgcacag cccgccagca atgcggtgag caatgccaca
     5041 ggaattaatc ttttcatggt gttacctcag taataaacgc taacgcttaa taagttaact
     5101 ctcttattaa cgagctgaat gccattttat cactggaggt tataaatctt gcggaatgaa
     5161 taatgacaaa ttatgtgaat tgtaaaaata gtgaagggag cgggaactat aga
//
LOCUS       DAZFLD010000262         5050 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.264, whole genome shotgun sequence.
ACCESSION   DAZFLD010000262 DAZFLD010000000
VERSION     DAZFLD010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5050
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.264"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(183..339)
                     /locus_tag="ACHKBL_004393"
                     /pseudo
     CDS             complement(183..339)
                     /locus_tag="ACHKBL_004393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
     gene            complement(539..964)
                     /locus_tag="ACHKBL_004394"
     CDS             complement(539..964)
                     /locus_tag="ACHKBL_004394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HFO5821161.1"
                     /translation="MRDIQQVLSRWGAWVVNNHESVAWSGVAAGFKGVIPSKVKSRPQ
                     CTDDDALIISNCMAQLNVNNSDLHDFLYDYYVFGMTLMGLGRKHERSDCWAGRVLQKA
                     EGVIEGMLIMQGIELEMDRYVERERSGAQVSQFSGHTGN"
     gene            complement(1043..1906)
                     /locus_tag="ACHKBL_004395"
     CDS             complement(1043..1906)
                     /locus_tag="ACHKBL_004395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO5821162.1"
                     /translation="MRTDNQVHKALFTIPTAAYSAVPANIKPLPEQRRITGHKQTDAY
                     LWILEVIHLNEAVHLDAAEAALEKLKITPEEASERYGRYLQEINVDPFQIAFATIGMD
                     NPAQAIRNARENIKKSSSVRATFGSYEAALDDVEAERIIRTSPKFIDDYYWGWTAAEK
                     KAGSIDGVRSNEIDDQRRAYVDGYRDVLPEPHTLSDVVREFIYWDWLYEMRQTAGRET
                     GDKYGFTGEHHESVYDRQFWLENLLGKIKPVTRDEAVEVCRWFLASGKDEYMEDNGSA
                     VILNLVGECEQ"
     gene            complement(1906..2874)
                     /locus_tag="ACHKBL_004396"
     CDS             complement(1906..2874)
                     /locus_tag="ACHKBL_004396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HFO5821163.1"
                     /translation="MKTAEAAKGHWPEILKHYGLPPVTGKKHFKGECPVCGARGKFRI
                     DDRDGTGTWICVCGSGDGMKLIALTQKRAFHEICAEIDRITGNEYRRDKPPVICTSES
                     LRSRVQRRFSALTALQGTSGAEYLRSRGIFSLPVEGVRFNSQQNYNGRMFQSLYALAT
                     DDKGELCYLHQTLLDGAKKADIGISARRLKSLQDDNYLDHARSVAIRMFPVASTLGIA
                     EGIETALSAHQIYKVNTWATINSGFMKKFRVPAGVTHLIIFADRDEYSATGMAAACEC
                     AHANLQARNDIQRVSVRWPDHDDFNNMLMNGDQVRELVFYRKKAVA"
     gene            complement(2876..4534)
                     /locus_tag="ACHKBL_004397"
     CDS             complement(2876..4534)
                     /locus_tag="ACHKBL_004397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFO5821164.1"
                     /translation="MLNIKPNFAQERALNMLRHDWKTYQSFMMYMPTGSGKTGLAAFI
                     TAGLVSRGMRVLFVVPYTILINQTAQRFVQYGLSGDEISFIWRDHPNNDPSRLIQIAS
                     ADTLIRRDFPQNIDLLIIDEAHLRKRRILTEIERLVAEGNVKVIGLSGTPFSSFLGRY
                     YQRLLKPTTIGELIQRGELSNYEFYAPTKPDLEGVKTKVSMEYGRDYDESQLAEIMCG
                     ADLVGDIVDNWLCNGRDLPTVAFCVNKTHASFVTMQFNKAGINAEVMVAETPHDERQL
                     MISRFETGATKIIVSVGVLVAGFDSDVRCIIYARPTKSEIRWLQAIGRGLRTAPGKES
                     CLIFDHSGTVHRLGFPENIEYDELPGKNDGMQVSSSRGNEIREEKLPKECPGCHFMKP
                     AGVYVCPKCGFKPLAGEDVNTDTRRNIRKLSKDEKIYTKSDKQAWWSQIKFYQRQRAS
                     LGRPVSDGWCAHTFREKFGEWPDGLSSFPMEITPEVSNYIRHKFIRFARGRSRAEKMT
                     EKSPEALSLSLLHGVRSGVPEGSESWQIMQAKQQLQKNRNSLSQ"
     gene            complement(4568..4936)
                     /locus_tag="ACHKBL_004398"
     CDS             complement(4568..4936)
                     /locus_tag="ACHKBL_004398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001271873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5821165.1"
                     /translation="MSAELKLVLINLLEEEFGHGMDKVTFDYVLQKKIKSLGCELQRC
                     FTVRLKGDRRGFIDLLVISPDGQRCAVEVDNRSPRQRSLMKIRALPEGISGFVFLRDG
                     KHPQRYIADGVDVIRATRFK"
BASE COUNT         1201 a         1398 c         1106 g         1345 t
ORIGIN      
        1 ggcagcataa aatcatcctt ttatgaaaat caacgttttt tgagcactcg cacacaaaaa
       61 attaccgcac gggttgcaac gacaacctgc attttgcgga gcatatgaca aataaagtac
      121 gggtgcgttg aggatgcctg acacatcaga ggtggcggga gattactccc ccgcctggtc
      181 tcttacttct cagattcgta gtctacgaag acagcgacct ccgtctggcc ggttcggatc
      241 cgtacctcgc agaggtcttt cctcgttacc agtgccgtca ctatgacagt taaacagatg
      301 acgatcaggg cgaattaaca tcgccttttg ctgcttcata gactgcttct ccttgccttc
      361 cggcacgtaa gaggctaacc tacggttgtg tgacatagat tgggcctcag attaatgtta
      421 agcgtcttgc aggacacgta atgttaactg gggcttttct ccgtctgcct tacggcagca
      481 tgcccgaggc agacaccctc aagcacccgc agtcattcta cagtaaaacc ccgcgctttc
      541 aatttcccgt atgtccggaa aactgactga cctgtgcccc tgatcgttca cgctcaacgt
      601 atctgtccat ttccagttct attccctgca taatcaacat cccctcaata acaccttcag
      661 ctttttgcag tacccgcccg gcccagcaat cagagcgctc atgcttacgg cccagtccca
      721 taagcgtcat cccgaacaca taataatcat acagaaaatc atgcaaatcg ctgttgttga
      781 cgttcagttg cgccatgcaa ttgctgataa tcagcgcatc atcatcggta cactgaggcc
      841 gtgatttaac ctttgatggg atcactccct taaatccggc agcgacgccg gaccatgcca
      901 cactctcatg attatttacc acccatgcac cccatcggga cagaacctgt tggatatcac
      961 gcattattct gtactccgct gtaaccatac tttttctgat ttgaatgctc agggggaaaa
     1021 tgtctactgg atatctccgg cttcattgct cacactcccc aaccagattg agaatgaccg
     1081 ctgaaccatt gtcttccatg tactcatctt ttccgcttgc cagaaaccag cgacacactt
     1141 caaccgcttc atcacgcgtc acaggcttta tttttcccag caaattttcg agccagaact
     1201 gccgatcata tactgactca tgatgttcac ctgtgaaacc gtatttgtcc cctgtttctc
     1261 tccccgctgt ctggcgcatc tcataaagcc agtcccagta aataaactca cgaacaacgt
     1321 ctgacaatgt atgaggctct ggcagtacat cacgatagcc atcaacatat gcacgacgct
     1381 gatcatcaat ttcatttgag cgaacaccgt caatacttcc ggcttttttc tctgccgcag
     1441 tccagcccca gtagtaatcg tcgataaatt tcggggaggt gcgaattatc cgctcagcct
     1501 ccacatcgtc gagtgctgcc tcatagctgc caaatgtggc cctgactgat gaggattttt
     1561 tgatattctc acgggcattc ctgatcgcct gcgccgggtt atccatgcct atggttgcga
     1621 aagcaatctg gaaaggatca acattaatct cctgcaaata acgtccatac cgttcactcg
     1681 cttcttccgg cgttattttg agtttctcca gtgctgcttc ggctgcgtcc agatgtacgg
     1741 cctcgttcag gtgaataacc tccagaatcc agagataagc atcagtctgt ttatgtccgg
     1801 tgatcctcct ttgttctggc agaggtttga tatttgccgg aacggcgctg tacgctgccg
     1861 tcgggatagt gaataaagct ttatgtacct gattgtccgt acgcatcacg caaccgcctt
     1921 ttttctgtaa aaaaccagtt cccgaacctg atcgccgttc atgagcatat tgttgaaatc
     1981 atcatgatca ggccaccgga cacttacccg ctgaatgtca tttcgggcct ggagatttgc
     2041 atgtgcgcat tcacaggccg cagccattcc ggtagcactg tattcatcgc gatcggcaaa
     2101 aataatcaga tgggtgactc ccgccggtac gcggaacttt ttcataaaac cactgttaat
     2161 ggttgcccag gtatttactt tgtaaatctg atgtgcggac aacgcggttt cgataccttc
     2221 agctataccc agtgtactgg ctaccggaaa catcctgata gcaactgacc gggcatggtc
     2281 cagatagtta tcgtcctgta aagatttgag ccttctggcg ctgataccaa tgtctgcttt
     2341 tttagctcca tcaagcaatg tctgatgcag gtaacacagt tctcccttgt catcggtcgc
     2401 cagggcataa agagactgga acatgcgccc gttataattt tgctggctgt tgaagcgaac
     2461 tccctcaacc ggaagactga atatgccacg ggaacgaaga tattcagcac cagatgttcc
     2521 ctgtagcgcc gtgagcgctg aaaatcgacg ctgaacgcgc gatctcaggc tttccgacgt
     2581 acagatcaca ggcggtttat ctctccggta ttcattcccc gtgatgcggt ctatttcagc
     2641 gcaaatttca tgaaacgctc ttttttgtgt cagagcgatc agtttcatgc cgtcaccgct
     2701 accacaaaca cagatccacg ttccggtgcc atcacggtca tcaatacgga acttacctct
     2761 ggcaccacaa accggacatt cccctttgaa gtgcttttta cccgtgactg gcggcagtcc
     2821 gtaatgtttt aaaatttcag gccagtggcc tttcgctgct tctgctgttt tcatcttact
     2881 gactcagact gtttctgttt ttctgaagtt gttgctttgc ctgcatgatc tgccacgact
     2941 cactgccttc cggcacccca gaccggacac catgaagcag ggacagagaa agcgcttcgg
     3001 gggatttttc cgtcatcttc tctgcccggc tacgccctct ggcaaaccgg atgaatttgt
     3061 gtctgatgta attactgacc tccggggtga tttccatcgg aaaactgctc agcccgtcag
     3121 gccattcgcc gaacttctcc cggaaagtgt gagcgcacca tccatcgctg accggtcgcc
     3181 ccagagaggc acgctgacgc tgatagaatt tgatctgact ccaccaggcc tgtttgtcgc
     3241 ttttggtgta aattttttcg tccttgctga gtttcctgat attgcggcgg gtatctgtgt
     3301 tcacatcctc accagccaga ggtttaaatc cgcatttcgg acagacataa acacctgccg
     3361 gtttcatgaa atgacacccc ggacattctt tcggcagttt ttcttcccga atttcgttac
     3421 cacgtgatga tgacacctgc atcccgtcgt ttttgccggg cagttcgtca tactcaatat
     3481 tttcaggaaa tcccaggcga tgaaccgtac cggaatggtc gaaaatcagg catgactcct
     3541 tgcctggcgc tgtacgcaat cctctcccaa tcgcctgaag ccagcgaatc tctgattttg
     3601 tcgggcgtgc gtaaatgatg caccggacat cactgtcaaa tcccgccacc agaacgccaa
     3661 cgctgacaat gattttcgtc gctcccgttt caaaacgact gatcattaac tgacgctcat
     3721 catgtggcgt ttcagcaacc atcacctctg cgttaattcc ggctttgttg aactgcatgg
     3781 tcacgaagct ggcatgcgtt ttattgacgc agaacgccac tgtcggtaaa tcccttccgt
     3841 tacacagcca gttatcgaca atatcgccaa ccagatccgc gccgcacata atctctgcca
     3901 gttgtgattc gtcgtaatcc ctgccgtact ccattgagac tttcgttttt acaccttcca
     3961 gatccggttt cgtgggcgca taaaattcgt aattgctcag ttcgccacgc tggatcagtt
     4021 cccctatagt ggtcggcttg agaagacgct ggtaataacg ccccaggaac gatgaaaagg
     4081 gtgtaccgga caacccaatc accttcacat ttccctctgc aaccagtcgt tcgatttccg
     4141 tgaggatgcg gcgcttacgg agatgcgctt catcaataat cagcaggtcg atattctgag
     4201 gaaaatcacg gcgaatcagc gtatccgcac tggcaatctg aatcagtcgt gacgggtcgt
     4261 tattgggatg atcacgccag ataaagctga tttcatcccc ggaaagcccg tactgaacaa
     4321 agcgctgtgc tgtctggttg atcaaaatgg tataaggaac gacaaacaga acgcgcatgc
     4381 cacggcttac cagaccagca gtgatgaacg ccgccaggcc ggttttacca ctccctgtag
     4441 gcatgtacat catgaaggac tgatatgttt tccagtcatg acgcaacata ttcagcgcac
     4501 gttcctgagc aaaattcggc ttgatattca gcatggtatt ctcccgatga cagggctgat
     4561 acagtgatta cttaaaccgg gtcgcccgga tgacatccac accatcggcg atataacgct
     4621 gtggatgttt gccatcacgg agaaaaacaa atcccgaaat gccttcagga agcgctcgaa
     4681 ttttcatcag tgaacgctga cggggcgatc ggttatccac ctcaactgcg caacgctgac
     4741 catcgggaga aataaccaga agatcgataa atcccctgcg atcacctttc agcctgacag
     4801 tgaaacagcg ttgcaactca cagcccaggg atttgatttt cttttgcaga acgtagtcga
     4861 atgtgacctt gtccattcca tgtccgaatt cctcttccag aaggttgatt agtaccagtt
     4921 tcagttcagc gctcatattt taaccatgat gtactagttt gctgatacga ccgttttttc
     4981 aggggtgccc ccctttagat cgagatctac ctaacctctg tacccgtctg ttggaagatc
     5041 ccgttccagt
//
LOCUS       DAZFLD010000263         5002 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.265, whole genome shotgun sequence.
ACCESSION   DAZFLD010000263 DAZFLD010000000
VERSION     DAZFLD010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5002
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.265"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..530
                     /locus_tag="ACHKBL_004399"
     CDS             <1..530
                     /locus_tag="ACHKBL_004399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001011500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type 4 pilus major pilin"
                     /protein_id="HFO5821166.1"
                     /translation="RSCLYSYFALKKEKEEDKGLTLLEIAGALIIIGIVVALAMSQMS
                     STMSSSDNQRELKNLQTIATKMKQYKFQGRYTGTDYIKTLTESGSLPADMIAGSNKAK
                     NAWGGDVTIAATSNKYGYTITSKAVPKENCVELINSLRSSSMFTKIKGQTPASVDPVT
                     VCSAATNDITLETSS"
     gene            578..1060
                     /locus_tag="ACHKBL_004400"
     CDS             578..1060
                     /locus_tag="ACHKBL_004400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HFO5821167.1"
                     /translation="MKWLFFSAVICPLFFALPAEAVATNTLLDSCFIQAGKRYQIAPD
                     LLKTIAQQESSLVATAINHNKNKSGKIISTDYGIMQINSVHIPELKKLGVIKDKNDLL
                     NNPCLNIQTGAWILARHFQRCGVNWECLGSYNAGFSKNNTARRMIYARKIHNRYFRQY
                     "
     gene            1020..1727
                     /locus_tag="ACHKBL_004401"
     CDS             1020..1727
                     /locus_tag="ACHKBL_004401"
                     /EC_number="3.4.23.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001114349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HFO5821168.1"
                     /translation="MPEKSTTGISDNINKYEVTVSALLSMTIIIIILAVILYLAYSTA
                     ALYLSAVHDTRPPRPVVYVCCLLAIVSVSLNGAYGVEATGLLFLSILTGLLTVMTLTD
                     IAVCRLPRIFTLSLIVLGAAFRYSLEELTYSLLNVSLWFGMTYLLRQFFITAKGTEAL
                     GLGDVFLIAGIAMWTQPQHTPLLITAAASGAFLFILLFCRRRRQQALPFAPFLCASLY
                     ALTLLPDSVFRTSEIFT"
     gene            1724..3097
                     /gene="pilV"
                     /locus_tag="ACHKBL_004402"
     CDS             1724..3097
                     /gene="pilV"
                     /locus_tag="ACHKBL_004402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shufflon system plasmid conjugative transfer
                     pilus tip adhesin PilV"
                     /protein_id="HFO5821169.1"
                     /translation="MKKFRHDDDKGFILLEILAGLIVIGIATPLIYSEIENWLNEQLY
                     QSAASHADAYNTAARNYIADNNATLHNGSLPANFTADDLIRKGYLKQGFNRSPFGQSY
                     ITGIRRNQTTGRLEALTCSTGGQNIKEDGLRSVAGQLPGLGGYIGKNGTATGAFGAWT
                     DKPGDYGLTCSAGHIAVVMTGDDLQESDRLYRFQVPGRPELNQMHTAINMGGNDINNA
                     GNLNGQKATVKGDITSEDGWLITRNNKGWMNTTHGGGFTMTDSQWIRAVNNKGITTDG
                     EIKGGKVSGGTIRSDGRLSTGEYLQLEKTATAGTSCSPDGLVGRDSKGAILSCQSGMW
                     AGFESYPVGSPIPWPSATPPQGYLVMNGQSFSCSRYPQLARAYPGCKLPDLRGVFIRG
                     WDNGRGLDGDRNRHLLSYQADQSGVYHERGGWLKGHHSGMPYWAQGSTTEMRPKNIAF
                     NYIVKAS"
     gene            3424..4590
                     /locus_tag="ACHKBL_004403"
     CDS             3424..4590
                     /locus_tag="ACHKBL_004403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000486835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFO5821170.1"
                     /translation="MFQKKTRKMSLSRALDKYLKTVSVHKKGHLQEFYRVNVIKRHPM
                     ADRYMDEITTIDIAGYRDQRLAQINPRTGRQITGNTVRLELALLSSLFNIARVEWGTC
                     RMNPVELVRKPKISNGRDRRLTSGEERRLSRYFRDKNPQLYVIFHLALETAMRQGEIL
                     SLRWEHLDLQHGVAHLPETKNGSPRDVPLSRKARNYLQILPQQINGNVFSYTSSGFKS
                     AWRTALLDLKIENLHFHDLRHEAISRLFELGTLNVMEIAAISGHRSLNMLKRYTHLRA
                     YHLVSKLDARRKQTSKISPYFVPYPATVRNRNGVFIVTLHDFDLESRAETRELAISHA
                     SVLLLRALAQAAQRGERVPTPGELPANIDERVMICPLTNSQFMTKANSIFISSS"
     gene            4632..4922
                     /locus_tag="ACHKBL_004404"
     CDS             4632..4922
                     /locus_tag="ACHKBL_004404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001597008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5821171.1"
                     /translation="MKHLSSRAATADNNRIVTIEDVYTIAFSQPIHTESALGQRWMLI
                     HTGEYESASDAKRFLDTLVTGHCACMVTPSPVLDHDTWLRIRLLMGEIRDAK"
BASE COUNT         1343 a         1306 c         1169 g         1184 t
ORIGIN      
        1 tcagatcatg tctttattct tattttgctt taaaaaaaga gaaagaggag gataaaggtt
       61 taacattact ggaaattgcc ggtgcactga ttatcatcgg tattgtggta gctctcgcaa
      121 tgagtcagat gtccagcaca atgtcatcca gtgataacca gcgtgaactg aaaaacctgc
      181 agactattgc aacaaaaatg aagcagtaca agttccaggg cagatatacc ggcacagatt
      241 atatcaaaac cctgacagaa agcggttccc tgccggctga tatgatcgcc ggaagtaaca
      301 aggccaaaaa tgcatgggga ggcgatgtca ccattgcagc aacaagtaat aaatacggtt
      361 ataccataac cagtaaagcc gtaccgaaag aaaactgtgt tgagctgatt aacagtctgc
      421 gcagttcatc catgttcaca aaaatcaaag gccagacacc tgccagtgtg gatccggtaa
      481 ctgtatgctc tgccgccaca aacgatatca cgctcgaaac cagcagttaa tatcctgaca
      541 cagcgccctc cggggcgctt ttccggagtg tatccgcatg aagtggttat ttttttctgc
      601 cgtgatttgt ccgttatttt tcgcccttcc cgctgaggct gtcgccacaa acacattact
      661 ggacagttgc tttattcagg ccggaaaacg ctaccagatt gcgcctgatc ttcttaaaac
      721 cattgcacag caggaaagca gtctggtcgc cactgcgata aatcacaata aaaacaaatc
      781 cggaaaaatc atcagtacgg attacgggat tatgcagatt aattcagtgc atattcctga
      841 gctgaaaaag cttggagtca ttaaagacaa aaatgacctg cttaataatc cctgcctgaa
      901 tattcagacc ggggcctgga tactggcccg ccattttcaa cgctgcggcg ttaactggga
      961 atgtctgggc agctataacg ccggtttcag caaaaataac acagcccggc gcatgatata
     1021 tgcccgaaaa atccacaacc ggtatttcag acaatattaa taaatacgag gtgacggtga
     1081 gcgcattact gagcatgaca ataataatca tcatcctggc ggttattctt tatcttgcat
     1141 acagcaccgc cgctctctac ctgtcagcag tacacgacac ccggccgccc aggccggtgg
     1201 tgtacgtgtg ttgccttctg gccatagtga gtgtcagcct taacggcgca tatggtgttg
     1261 aagccacggg gctgttattc ctttccattc tgacgggatt attaactgta atgacgctga
     1321 cggacatcgc cgtctgtcgg cttccccgga tattcacgct ttcactgatt gttctggggg
     1381 cagcgttcag gtacagcctg gaggagctca catacagcct cctcaacgta tcactctggt
     1441 tcggcatgac atacctgcta cggcagtttt tcataacggc aaaaggaaca gaagcactcg
     1501 gccttggtga tgtattcctg atagcaggaa ttgcaatgtg gacacagcca caacacacgc
     1561 cgctgctaat caccgccgca gcttccgggg catttctgtt tatcctcctc ttctgccgtc
     1621 gcagacgtca gcaggcactg ccgttcgccc cgtttctctg cgcatcactg tatgcactaa
     1681 ccctcctccc tgattccgtt ttccgcactt cggagatatt cacgtgaaaa agttcagaca
     1741 cgatgacgac aaaggtttta ttctgcttga aatactggcg ggactgatag tgattggtat
     1801 cgccacgccg ctgatttatt ccgaaataga aaactggctg aatgaacagc tttaccagag
     1861 tgctgcttct catgcagatg cctacaatac cgcagccaga aattacattg ccgataacaa
     1921 tgccactctg cacaacggca gccttccggc aaactttaca gccgatgatt tgattcgcaa
     1981 gggatacctg aaacagggat ttaatcgttc tccgtttggg cagagttata tcacgggtat
     2041 ccgccgtaac cagactacag gacgtcttga agccctgacc tgctcaacag gggggcagaa
     2101 tatcaaagaa gatggtctgc gcagtgtggc aggacagctt cccgggctgg gtggttatat
     2161 tggtaaaaac ggaacagcaa ccggcgcatt cggcgcctgg acagacaaac cgggggatta
     2221 cggactcacc tgttcggcag gtcacattgc cgtcgtcatg acgggtgatg atctgcagga
     2281 aagcgacagg ttgtatcgtt ttcaggtccc ggggagacca gagctgaacc agatgcacac
     2341 cgccatcaac atgggcggaa atgacatcaa taacgccggt aacctcaatg gtcagaaagc
     2401 cacggtgaaa ggcgatataa ccagcgaaga cggctggctt atcaccagaa acaacaaggg
     2461 gtggatgaac acgacgcacg gaggcggttt caccatgact gacagccagt ggatacgcgc
     2521 cgtcaataac aaagggatca ccaccgatgg tgaaatcaaa ggaggcaaag tcagcggtgg
     2581 aacgatccgg tctgacggtc gtctttccac aggtgagtat ttacagctgg aaaaaacagc
     2641 caccgcaggt acttcctgct caccggacgg gcttgtaggc cgcgacagta aaggggcaat
     2701 actttcgtgc caatccggta tgtgggcagg ttttgaatcc taccctgtcg gaagccctat
     2761 accgtggcca tcagccaccc ctccacaagg ctacctggtg atgaacgggc agtcattttc
     2821 atgttcccgg tatcctcagc ttgcccgtgc ttatcccggg tgtaagctgc cggatttgcg
     2881 cggtgttttt atccgtggct gggacaacgg acgtgggctg gatggagatc ggaacagaca
     2941 tctcctgagt tatcaggccg atcaatccgg tgtctatcat gagcgtgggg gatggctgaa
     3001 agggcatcac agcggtatgc cttactgggc ccagggaagc acaacggaaa tgcgccccaa
     3061 aaacattgcc tttaactaca tcgtcaaagc aagctgatgc atttaaggcg tattaacttt
     3121 taacttacag caacgtattt tgacccagac catgcttccc gaataccccg gtttacctct
     3181 gtacgctctg aatcctgccg caccatagcc tgctttgccg tcacagacgg caaagccgtt
     3241 tgagccacta ccccagttcg cataaaccgg ttttctggag tcttcttctg cgtcataaca
     3301 ggccgaataa tcaaaaacca gtgcatcgct gatattattc ttccagacac cggattggca
     3361 cgaaagtatt gcatttctgt atccagcctc ctaccttccc cgtttttatc gtggaaggca
     3421 gatatgtttc aaaaaaaaac tcgcaaaatg tccctcagcc gggcactgga caaatacctg
     3481 aagactgttt ccgtccataa aaaaggtcat ctgcaggagt tttatcgcgt caatgtgatt
     3541 aaacgtcacc ctatggccga tcgctatatg gatgaaatca ctaccattga tatcgccggt
     3601 taccgggatc agcgtttagc tcaaattaac ccacgaacag ggcgtcaaat caccggaaat
     3661 accgtccgtc ttgaacttgc ccttttatca tcattattca atatcgcccg tgtggaatgg
     3721 ggaacatgcc gtatgaatcc tgttgaactg gtccgaaaac cgaaaatcag taatggccgc
     3781 gaccgccgac tgacttctgg tgaagaacgc cgcctttccc gctatttcag ggacaaaaat
     3841 ccgcagctct acgttatctt ccatctggct ctggaaacag caatgcgcca gggagaaatt
     3901 ctctcattac gctgggaaca cctcgatttg cagcacggcg tggcgcatct tcctgaaacg
     3961 aaaaatggct caccacgcga cgtaccactc tcccggaaag cacgtaacta cctgcaaatt
     4021 ctgccacaac agataaacgg caatgtgttc agttacacct cgtccggttt caaaagcgcc
     4081 tggcgtaccg ctcttctgga tctgaaaata gaaaatctgc attttcacga tctccgccat
     4141 gaggccatca gccgtctgtt cgagctcggc acactgaacg ttatggagat tgcagccatt
     4201 tccggtcacc gctccctgaa catgttgaaa cggtacacac atctccgggc atatcacctg
     4261 gtcagtaaac tcgatgcaag gcgtaaacag accagcaaaa tttcccctta ttttgttccc
     4321 taccctgcaa ctgtcagaaa cagaaacgga gtgttcattg tgacactgca cgatttcgac
     4381 cttgaaagcc gtgctgaaac ccgggaactg gcaatatctc acgccagcgt attgttgctg
     4441 cgcgcactgg cccaggctgc tcagcggggt gaacgcgtcc ccacaccagg cgagctaccc
     4501 gcaaacattg atgaacgggt catgatttgt ccgttaacaa acagccagtt catgacaaaa
     4561 gcgaacagta ttttcatatc ctcctcctga acaaataatt tttgttcaaa attaacgcta
     4621 tgagaccctt tatgaaacac ttatcctccc gtgcagccac tgcggataat aatcgtatcg
     4681 ttacaattga agacgtttac acaatcgcat tctcgcaacc aattcacacc gaatcagcgt
     4741 tggggcaaag gtggatgttg atccatactg gtgaatatga aagtgccagc gatgcaaaac
     4801 gcttccttga tacgctcgtc accgggcact gtgcttgcat ggtaacacca tcacctgttc
     4861 tggatcatga cacctggctg cgtatccgcc tgctgatggg cgagataagg gatgccaaat
     4921 aaacattcat ggccccaaca cataaaaata acgccgggga ctctggcaga ggtaccattc
     4981 tttttacata gagccaatat at
//
LOCUS       DAZFLD010000264         4981 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.266, whole genome shotgun sequence.
ACCESSION   DAZFLD010000264 DAZFLD010000000
VERSION     DAZFLD010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4981)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4981)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4981
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.266"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..193
                     /locus_tag="ACHKBL_004405"
     CDS             <1..193
                     /locus_tag="ACHKBL_004405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5821172.1"
                     /translation="RTALQAYLFLIFIKMLRQLTFLLPTKFQRGKKLRTQRYRGLQSK
                     EKRKLSLLTTKFSDEVMGE"
     gene            605..2587
                     /gene="chuA"
                     /locus_tag="ACHKBL_004406"
                     /gene_synonym="shuA"
     CDS             605..2587
                     /gene="chuA"
                     /locus_tag="ACHKBL_004406"
                     /gene_synonym="shuA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent heme/hemoglobin receptor
                     ChuA/ShuA"
                     /protein_id="HFO5821173.1"
                     /translation="MSRPQFTSLHLSLLALAVSATLPTFAFATETMTVTATGNARSSF
                     EAPMMVSVIDTSAPENQTATSATDLLRHVPGITLDGTGRTNGQDVNMRGYDHRGVLVL
                     VDGVRQGTDTGHLNGTFLDPALIKRVEIVRGPSALLYGSGALGGVISYDTVDAKDLLQ
                     EGQSSGFRVFGTGGTGDHSLGLGASAFGRTENLDGIVAWSSRDRGDLRQSNGETAPND
                     ESINNMLAKGTWQIDSAQSLSGLVRYYNNDAREPKNPQTVEASDSSNPMVDRSTIQRD
                     AQLSYKLAPQGNDWLNADAKIYWSEVRINAQNTGSSGEYREQITKGARLENRSTLFAD
                     SFASHLLTYGGEYYRQEQHPGGATTGFPQAKIDFSSGWLQDEITLRDLPITLLGGTRY
                     DSYRGSSDGYKDVDADKWSSRAGMTINPTNWLMLFGSYAQAFRAPTMGEMYNDSKHFS
                     IGRFYTNYWVPNPNLRPETNETQEYGFGLRFDDLMLSNDALEFKASYFDTKAKDYIST
                     TVDFAAATTMSYNVPNAKIWGWDVMTKYTTDLFSLDVAYNRTRGKDTDTGEYISSINP
                     DTVTSTLNIPIAHSGFSVGWVGTFADRSTHISSSYSKQPGYGVNDFYVSYQGQQALKG
                     MTTTLVLGNAFDKEYWSPQGIPQDGRNGKIFVSYQW"
     gene            2636..3664
                     /gene="chuS"
                     /locus_tag="ACHKBL_004407"
     CDS             2636..3664
                     /gene="chuS"
                     /locus_tag="ACHKBL_004407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hematinate-forming heme oxygenase ChuS"
                     /protein_id="HFO5821174.1"
                     /translation="MNHYTRWLELKEQNPGKYARDIAGLMNISEAELAFARVTHDAWR
                     MRGDIREILAALESVGETKCICRNEYAVHEQVGAFTNQHLNGHAGLILNPRALDLRLF
                     LNQWASVFHIKENTTRGERQSIQFFDHQGDALLKVYATDNTDMAAWSELLARFIADEN
                     MPLELKAVDAPVVQTRADASVVEQEWRAMTDVHQFFTLLKRHNLTRQQAFNLVADDLA
                     CKVSNSALAQILESAQQDGNEIMVFVGNRGCVQIFTGVVEKVVPMKGWLNIFNPTFTL
                     HLLEESIAEAWVTRKPTSDGYVTSLELFAHDGTQIAQLYGQRTEGEQEQAQWRKQIAS
                     LIPEVVTA"
     gene            complement(3725..4255)
                     /gene="dctR"
                     /locus_tag="ACHKBL_004408"
     CDS             complement(3725..4255)
                     /gene="dctR"
                     /locus_tag="ACHKBL_004408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HFO5821175.1"
                     /translation="MFLIITRDTMFFTAMKNILSKGNVVHIQNEEEIDVMLHQHAFVI
                     IDTLMNNVFHSNLLTQIERLKPVHVIIFSPFNIKRCLGKVPVTFVPRTITIIDFVALI
                     NGSYCSVPEADVSLSRKQHQVLSCIANQMTTEDILEKLKISLKTFYCHKHNIMMILNL
                     KRINELVRHQHIDYLV"
     gene            complement(4408..4974)
                     /gene="slp"
                     /locus_tag="ACHKBL_004409"
     CDS             complement(4408..4974)
                     /gene="slp"
                     /locus_tag="ACHKBL_004409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein Slp"
                     /protein_id="HFO5821176.1"
                     /translation="MNMTKGALILSLSFLLAACSSIPQNIKGNNQPDIQKSFVAVHNQ
                     PGLYVGQQARFGGKVINVINGKTDTLLEIAVLPLDSYAKPDIEANYQGRLLARQSGFL
                     DPVNYRNHFVTILGTIQGEQPGFINKVPYNFLEVNMQGIQVWHLREVVNTTYNLWDYG
                     YGAFWPEPGWGAPYYTNAVSQVTPELVK"
BASE COUNT         1333 a         1112 c         1223 g         1313 t
ORIGIN      
        1 acgcacggcg ttacaagcgt atttattttt gatttttatc aagatgcttc gtcagctaac
       61 gttcttgtta ccgactaaat ttcagcgcgg gaaaaagctg agaacccaga gatatcgcgg
      121 cttgcagagc aaagagaaga gaaaattatc actgttaacg acaaagttta gtgatgaggt
      181 catgggggaa taaccataaa aacaaagtgg tttccagggc gcaggataag tttcataacc
      241 ccctgtttat tgtgttaatg gcggttaaaa aaaagcttga tcataaaaaa ttgatcgata
      301 ataattctca ttatcacgtt tgcatttaac aaaatacggc catatggata acaaagccat
      361 atcatttaca aggggtaacg cacagcgctc ttcactcctg atggggcata tcggtttgcc
      421 tgacaaagag ggcgtgcttt tgcgtatttg ccgagatacc aggcaatttc atgggatata
      481 aacgcctgta aactcacccg gcgaatatta tcgctctgaa ttttaaataa atttattttt
      541 cgccagccac cccggtatta acccaggcag ccagcatttt ttattttcct catggagaat
      601 cgctatgtca cgtccgcaat ttacctcgtt gcatttgagt ttgttggctt tggctgtttc
      661 tgccaccttg ccaacgtttg cttttgctac tgaaaccatg accgttacgg caacggggaa
      721 tgcccgtagt tccttcgaag cgcctatgat ggtcagcgtc atcgacactt ccgctcctga
      781 aaatcaaacg gctacttcag ccaccgatct gctgcgtcat gttcctggaa ttactcttga
      841 tggtaccgga cgaaccaacg gtcaggatgt aaatatgcgt ggctatgatc atcgcggcgt
      901 gctggttctt gtcgatggtg ttcgccaggg aacggatacc ggacacctga atggcacttt
      961 tctcgatccg gcgctgatca agcgtgttga gattgttcgc ggaccttcag cattactgta
     1021 tggcagtggc gcgctgggtg gagtgatctc ctacgatacg gtcgatgcaa aagatttatt
     1081 gcaggaagga caaagcagtg gttttcgtgt ctttggtact ggcggcacgg gggaccatag
     1141 cctgggatta ggcgcgagcg cgtttgggcg aactgaaaat ctggatggta ttgtggcctg
     1201 gtccagtcgc gatcggggtg atttacgcca gagcaatggt gaaaccgcgc cgaatgacga
     1261 atccattaat aacatgctgg cgaaagggac ctggcaaatt gattcagctc agtctctgag
     1321 cggtttagtg cgttactaca acaacgacgc gcgtgaacca aaaaatccgc agaccgttga
     1381 agcttctgat agcagcaacc cgatggtcga tcgttcaaca attcaacgcg atgcgcagct
     1441 ttcttataaa ctcgccccgc agggtaacga ctggttaaat gcagatgcaa aaatttactg
     1501 gtcggaagtc cgtattaatg cgcaaaacac ggggagttcc ggcgagtatc gtgaacagat
     1561 aacaaaagga gcaaggctgg agaaccgttc cactctattt gccgacagtt tcgcttctca
     1621 cttactgaca tatggcggtg agtattatcg tcaggaacaa catccgggtg gcgcgacgac
     1681 gggcttcccg caagcaaaaa tcgattttag ctctggctgg ctacaagatg agatcacctt
     1741 acgcgatctg ccgattaccc tgcttggcgg aacccgctat gacagttatc gcggtagcag
     1801 cgacggctac aaagatgttg atgccgacaa atggtcatct cgcgcgggga tgactatcaa
     1861 cccgactaac tggctgatgt tatttggctc atatgctcag gcattccgcg ccccgacgat
     1921 gggcgaaatg tataacgatt ctaagcactt ctcgattggt cgcttctata ccaactattg
     1981 ggtgccaaac ccgaacttac gtccggaaac taacgaaact caggagtacg gttttgggct
     2041 gcgttttgat gacctgatgt tgtccaatga tgctctggaa tttaaagcca gctactttga
     2101 taccaaagcg aaggattaca tctccacgac cgtcgatttc gcggcggcga caactatgtc
     2161 gtataacgtc ccgaacgcca aaatctgggg ttgggatgtg atgacgaaat ataccactga
     2221 tctgtttagc cttgatgtgg cctataaccg tacccgcggc aaagacaccg ataccggcga
     2281 atatatctcc agtattaacc cggataccgt taccagcacc ctgaatattc cgatcgctca
     2341 cagtggcttc tctgttgggt gggttggtac gtttgccgat cgctcaacac atatcagcag
     2401 cagttacagc aaacaaccag gctatggcgt gaatgatttc tacgtcagtt atcaaggaca
     2461 acaggcgctc aaaggtatga ccactacttt ggtgttgggt aacgctttcg acaaagagta
     2521 ctggtcgcca caaggcatcc cacaggatgg tcgtaatgga aaaattttcg tgagttatca
     2581 atggtaatca tctgccccga tatttcgggg catttatctg gaaggaagag agacaatgaa
     2641 ccactacaca cgctggcttg agttaaaaga acaaaatccc ggaaagtacg cgcgtgacat
     2701 cgcaggttta atgaatatta gcgaagcaga actggcattt gcgcgcgtca cgcacgatgc
     2761 gtggcggatg cgcggcgata tccgtgaaat tctggcggcg ctcgaaagtg ttggcgagac
     2821 caaatgtatt tgtcgtaatg aatatgcagt ccatgagcaa gttggtgcgt tcacaaacca
     2881 gcatttgaac ggacatgccg gattgatcct caatccacgc gcgctggatt tgcgtctgtt
     2941 tctcaatcaa tgggccagtg tttttcacat taaagaaaat acgactcgtg gcgaacgcca
     3001 gagtattcag ttctttgatc atcagggcga tgcattacta aaagtttatg ccaccgacaa
     3061 taccgatatg gcggcatgga gtgagcttct ggcacggttt atcgccgatg agaatatgcc
     3121 gcttgagtta aaagccgttg atgcgccagt tgttcaaacg cgagccgatg ccagtgtggt
     3181 cgagcaagag tggcgggcga tgaccgacgt tcatcagttt tttacgttgc tcaagcgcca
     3241 caacctgacg cgccaacagg cgttcaatct ggtggcagat gatttggcct gcaaagtatc
     3301 caacagtgcg ttggcgcaaa ttcttgaatc tgcacagcag gatggcaatg aaatcatggt
     3361 gtttgttggc aaccgtggct gcgtacagat tttcactggt gtggtagaaa aagtggtgcc
     3421 aatgaaaggt tggctgaata ttttcaaccc gacgtttact cttcatctat tagaagagag
     3481 cattgctgaa gcctgggtta cccgtaaacc gaccagcgat ggctacgtaa ccagtctgga
     3541 attgtttgcc catgatggta cgcagatagc gcaactttat ggtcaacgta cagaaggcga
     3601 acaggagcaa gcgcaatggc gtaagcaaat tgcttcgctg ataccggaag tcgttactgc
     3661 ataacgtata attaacggga ggtctggcta tttattgctg ggcctcttta tattgatcaa
     3721 tcgttcacac cagataatca atatgctgat ggcgtaccaa ctcattgatc cgcttaagat
     3781 tgaggatcat catgatattg tgtttatggc agtagaacgt ttttagcgat attttcagtt
     3841 tctccagaat atcttccgtt gtcatttgat tcgcaatgca gctcagaacc tgatgctgct
     3901 tgcgcgaaag ggacacatcc gcttcaggca cagagcagta actgccattg atgagtgcga
     3961 caaagtcgat gatggtgata gttcgcggaa caaaggtcac cggtactttc cccaggcagc
     4021 gtttaatatt aaagggagag aaaataatca catggacagg ttttaatcgt tcaatctgag
     4081 tgagcaaatt agaatgaaat acattattca ttaatgtatc aataatgacg aaggcatgct
     4141 gatgcaacat tacgtcgatc tcttcttcgt tctgtatatg aacgacatta cctttactca
     4201 gaatgttttt catcgcagtg aagaacatcg tatccctggt aattataaga aacataaccg
     4261 actcctggtg cggacattaa ctttataatc agtttgccaa aggtaattaa caggacaggg
     4321 cttataataa tagttagctg gctgtgtatt aatttgatgt aatgcatgat gcagatgctg
     4381 agaaagcatc tgcatctctc ggtggtgtta tttgaccagc tcaggtgtta cttgactcac
     4441 cgcattggtg tagtaaggcg cgccccagcc cggttccggc cagaatgcac catagccgta
     4501 atcccacagg ttataggtgg tattaaccac ttctctcaaa tgccacacct ggatgccctg
     4561 catattcact tccaggaagt tatacgggac tttattgata aagccaggtt gttcaccctg
     4621 aatggtaccg aggatggtaa caaagtgatt acgatagttc actggatcaa ggaagccgct
     4681 ttgtctggcg agcagtcggc cctgatagtt ggcttcaata tcaggcttcg catagctatc
     4741 caacggtaat acagcgattt ctaacaacgt atccgttttg ccattgataa cgttgataac
     4801 cttcccgcca aagcgcgctt gttgaccaac atataacccc ggctggttat gaacagcaac
     4861 aaaacttttt tgaatatcag gttggttatt gcctttgata ttttgcggaa ttgaactaca
     4921 tgcggcaagc aaaaatgaaa ggctgaggat gagtgcacct tttgtcatgt tcatgttact
     4981 a
//
LOCUS       DAZFLD010000265         4972 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.267, whole genome shotgun sequence.
ACCESSION   DAZFLD010000265 DAZFLD010000000
VERSION     DAZFLD010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4972)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4972)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4972
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.267"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(12..470)
                     /locus_tag="ACHKBL_004410"
     CDS             complement(12..470)
                     /locus_tag="ACHKBL_004410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HFO5821177.1"
                     /translation="MTIWVDADACPNVIKEILYRAAERMQMPLVLVANQSLRVPPSRF
                     IRTLRVAAGFDVADNEIVRQCEAGDLVITADIPLAAEAIEKGAAALNPRGERYTPATI
                     RERLTMRDFMDTLRASGIQTGGPDSLSQRDRQAFAAELEKWWLEVQRSRG"
     gene            590..1399
                     /gene="proC"
                     /locus_tag="ACHKBL_004411"
     CDS             590..1399
                     /gene="proC"
                     /locus_tag="ACHKBL_004411"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HFO5821178.1"
                     /translation="MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPDKVTA
                     LHDQFGINAAESAQEVAQIADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGITL
                     DQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
                     AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRAQAYKFAAQAVMGSAKMVLET
                     GEHPGTLKDMVCSPGGTTIEAVRVLEEKGFRAAVIEAMTKCMEKSEKLSKS"
     gene            complement(1416..2531)
                     /gene="adrA"
                     /locus_tag="ACHKBL_004412"
                     /gene_synonym="dgcC"
     CDS             complement(1416..2531)
                     /gene="adrA"
                     /locus_tag="ACHKBL_004412"
                     /gene_synonym="dgcC"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase AdrA"
                     /protein_id="HFO5821179.1"
                     /translation="MFPKIMNDENFFKKAAAHGEEPPLTPQNEHQRSGLRFARRVRLP
                     RAVGLAGMFLPIASTLVSHPSPGWWWLLLVGWAFVWPHLAWQIASRAVDPLSREIYNL
                     KTDAVLAGMWVGVMGVNALPSTAMLMIMCLNLMGAGGPRLFVAGLVLMVVSCLVTLEL
                     TGITVSFNSAPLEWWLSLPIIVIYPLLFGWVSYQTATKLAEHKRRLQVMSTRDGMTGV
                     YNRRHWETMLRNEFDNCRRHNRDATLLIIDIDHFKSINDTWGHDVGDEAIVALTRQLQ
                     ITLRGSDVIGRFGGDEFAVIMSGTPAESAITAMLRVHEGLNTLRLPNTPQVTLRISVG
                     VAPLNPQMSHYREWLKSADLALYKAKKAGRNRTEVAA"
     gene            complement(2633..2953)
                     /gene="psiF"
                     /locus_tag="ACHKBL_004413"
     CDS             complement(2633..2953)
                     /gene="psiF"
                     /locus_tag="ACHKBL_004413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiF"
                     /protein_id="HFO5821180.1"
                     /translation="MKITLLVTLLFGLVFLTTVGAAERTLTPQQQRMTSCNQQATAQA
                     LKGDARKTYMSDCLKNSKSAPGEKSLTPQQQKMRECNNQATQQSLKGDDRNKFMSACL
                     KKAA"
     gene            complement(3072..4487)
                     /gene="phoA"
                     /locus_tag="ACHKBL_004414"
     CDS             complement(3072..4487)
                     /gene="phoA"
                     /locus_tag="ACHKBL_004414"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HFO5821181.1"
                     /translation="MKQSTIALALLPLLFSPVTKARTPEMPVLENRAAQGDITAPGGA
                     RRLTGDQTAALRDSLSDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPL
                     TGQYTHYALNKKTGKPDYVTDSAASATAWSTGVKTYNGALGVDIHEKDHPTILEMAKA
                     AGLATGNVSTAELQDATPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLL
                     NARADVTLGGGAKTFAETATVGEWQGKTLREQAQARGYQLVSDAASLNAVTEANQQKP
                     LLGLFADGNMPVRWLGPKATYHGNIDKPAVTCTPNPQRNDSVPTLAQMTDKAIELLSK
                     NEKGFFLQVEGASIDKQDHAANPCGQIGETVDLDEAVQRALEFAKKDGNTLVIVTADH
                     AHASQIVAPDTKAPGLTQALNTKDGAVMVMSYGNSEEDSQEHTGSQLRIAAYGPHAAN
                     VVGLTDQTDLFYTMKAALGLK"
     gene            complement(4587..4847)
                     /gene="iraP"
                     /locus_tag="ACHKBL_004415"
     CDS             complement(4587..4847)
                     /gene="iraP"
                     /locus_tag="ACHKBL_004415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraP"
                     /protein_id="HFO5821182.1"
                     /translation="MKNLIAELLFKLAQKEEESKELCAQVEALEIIVTAMLRNMAQND
                     QQRLIDQVEGALYEVKPDASIPDDDTELLRNYVKKLLKHPRQ"
BASE COUNT         1154 a         1347 c         1223 g         1248 t
ORIGIN      
        1 ataaattaca tttagccacg actacgttgc acttccagcc accacttctc cagctccgca
       61 gcaaaagcct ggcggtcacg ttgtgaaaga ctatctggtc ctccggtctg gatcccactg
      121 gcacgtaagg tatccataaa atcgcgcatc gtcaggcgct cacgaatggt cgctggcgtg
      181 taacgttcgc cgcgcggatt aagcgccgcc gcgcctttct cgatggcttc agcagccaaa
      241 ggtatatctg cggtgatcac caaatcgccc gcttcacact gccgaacaat ttcgttatca
      301 gcaacgtcga aacctgccgc gacgcgcagc gtacgaataa atcgcgatgg cggcacgcgt
      361 aaactctggt ttgctaccag taccagcggc atctgcatac gttccgccgc gcgatacaaa
      421 atctctttaa ttacattggg acacgcgtcg gcatccaccc aaattgtcat aaagtcatcc
      481 tttgttgggt aatcctctat tgtgtcgcgc ttttgccttc cggcatagtt ctgtttatgc
      541 ttctgccagc gattatcaaa acaatgaatt tcacggcagg agtgaggcaa tggaaaagaa
      601 aatcggtttt attggctgcg gcaatatggg aaaagccatt ctcggcggtc tgattgccag
      661 cggtcaggtg cttccagggc aaatctgggt atataccccc tccccggata aagtcaccgc
      721 cctgcatgac cagttcggca tcaacgccgc agaatcggcg caagaagtag cgcaaatcgc
      781 cgacatcatt tttgccgccg ttaaaccagg catcatgatt aaagtgctta gcgaaatcac
      841 ctccagcctg aataaagact ctctggtcgt ttctattgct gcaggtatca cgctcgacca
      901 gcttgcccgc gcgctgggcc atgaccggaa aattatccgc gccatgccga acactcccgc
      961 gctggttaat gccgggatga cctccgtaac gccaaacgcg ctggtaaccc cagaagatac
     1021 cgctgatgtg ctgaatattt tccgctgctt tggcgaagcg gaagtgattg ctgagccgat
     1081 gatccacccg gtggtcggtg tgagcggttc ttcgccagcc tacgtcttta tgtttatcga
     1141 agcgatggcc gacgccgccg tgctgggcgg gatgccacgc gcccaggcgt ataaatttgc
     1201 cgctcaggcg gtcatgggtt ccgcaaaaat ggtgctggaa acgggagaac atccgggaac
     1261 actgaaagat atggtctgct caccgggagg caccaccatt gaggcggtac gcgtactgga
     1321 agagaaaggc ttccgtgccg cagtgatcga agcgatgacg aagtgtatgg aaaaatcaga
     1381 aaaactcagc aaatcctgat ggcttttgcc ggacgtcagg ccgccacttc ggtgcggtta
     1441 cgtccggctt tctttgcttt gtagagcgcc aaatctgccg atttcaacca ctcgcgatag
     1501 tgactcattt gtggattcag cggcgcaacc cccacactaa tccgtaaagt tacctgtggc
     1561 gtattcggca agcgtaacgt gtttagccct tcatgcaccc gtaacatggc ggtgatggcg
     1621 ctctctgctg gcgtaccgga catgattact gcaaactcat cgccgccaaa ccgaccaatc
     1681 acatcgctac cgcgtagggt aatttgtaac tgtcgggtaa gcgccacaat cgcttcatca
     1741 cccacatcat ggccccaggt atcgttgatg ctcttgaaat ggtcgatatc gataatcagt
     1801 aacgttgcat cgcgattatg ccgccgacag ttatcaaatt cattgcgtaa catagtttcc
     1861 caatgacgtc ggttatacac gcccgtcata ccgtcgcggg tactcatgac ctgcaacctg
     1921 cgtttatgtt ccgccagttt agtcgccgtc tggtagctga cccagccaaa cagcagagga
     1981 taaatgacaa taatgggaag ggagagccac cattccagcg gtgcgctatt gaacgacacg
     2041 gtgatgcccg tcagctcgag ggtgacaagg caggagacca ccatcaacac cagacccgcg
     2101 acaaacagac gggggccgcc tgcccccatc aaattcagac acataatcat caacatcgcg
     2161 gtggaaggca gtgcgtttac gcccattacg cctacccaca ttcccgctaa tactgcatcg
     2221 gtttttaagt tgtaaatttc ccggctaagc ggatcgacgg ccctgctcgc tatttgccag
     2281 gctaaatgcg gccagacgaa cgcccagccg accaacaaca gccaccacca gcccggcgac
     2341 ggatgcgaaa ccagcgttga agcaatcggt aagaacatgc cagccaggcc aaccgcgcgg
     2401 ggtagtctga cgcgacgggc aaagcgcagc ccggaccgct gatgttcgtt ttgaggagtt
     2461 aaaggaggtt cctccccatg cgccgccgct tttttgaaaa agttttcatc attcattatt
     2521 tttgggaaca ttcagaaaca attttcccaa attatagaga cggggctaaa ggcagaagta
     2581 tgaaatttcg ggtgagccat tttcgtagct cacccgttag cactatcagg tatcaggcgg
     2641 ctttcttgag gcaggcgctc ataaacttat tacgatcatc ccctttcaga gattgttgcg
     2701 ttgcttgatt attacattcg cgcatctttt gctgctgtgg cgtcaaactt ttttcaccag
     2761 gcgcagactt gctgttcttc aggcaatcac tcatgtaggt cttacgagca tcccctttca
     2821 acgcctgcgc cgtcgcctgc tgattacagg aggtcatacg ctgttgttgc ggggttaagg
     2881 ttctctcggc agcgccgacg gtggttaaaa aaaccagtcc gaaaagcaag gtaaccagta
     2941 atgttatttt catcgcacca tccctcttca tgttttaacc atgagcgttt gcgcccgtga
     3001 tctgccatta agtctggttg ctaacagcaa aaaaaccacc cgacagcgaa aattcaccgc
     3061 cgggcgcggt tttatttcag ccccagggcg gctttcatgg tgtagaagag atcggtctgg
     3121 tcggtcagtc caacgacatt ggcggcatgt gggccatacg ctgcaatacg caactgactg
     3181 ccggtatgtt cttgtgaatc ctcttcggag ttcccgtaac tcatcaccat cactgcgcca
     3241 tctttggtat ttagcgcctg ggtgaggccc ggcgctttgg tgtccggcgc gacaatctgg
     3301 ctggcgtggg cgtgatcagc ggtgactatg accagcgtgt tgccatcctt tttagcgaat
     3361 tccagcgcac gttgtacggc ttcatcgaga tcgaccgtct cgccaatttg cccacaagga
     3421 ttcgcagcgt gatcctgttt atcgatggat gcaccttcaa cttgcaggaa aaaacctttc
     3481 tcatttttac tcaacaattc aatggctttg tcggtcatct gcgccagggt cggtacactg
     3541 tcattacgtt gcggattagg cgtacaggta actgcgggct tatcgatatt gccgtggtac
     3601 gttgctttcg gtcctagcca gcgcactggc atattgccgt cagcaaacag gccaagcagg
     3661 ggtttttgct gattcgcttc cgtcaccgca tttagcgagg cagcatcact caccaactga
     3721 taaccacgcg cctgtgcctg ttcacgcagc gtttttccct gccattcgcc gacggttgcc
     3781 gtttcagcaa aggtttttgc gccgccgcca agcgtaacat cggcacgagc gttaagcagc
     3841 tgttcggtaa tcgatccttt tccgcctttt tccagagcgt tacccggaca tttttcactg
     3901 gtcgcgctcg gaccgtagca tttgcgcgag gtcacatgcg ccaccagcgc ggcgggcgtg
     3961 gcatcctgca actctgcggt agaaacgtta ccggtcgcca gacctgcggc ttttgccatt
     4021 tccagaatcg ttgggtgatc tttttcgtga atatcgacgc ccagcgcacc gttataggtt
     4081 ttgacaccag ttgaccaggc ggttgctgat gcagccgagt cggtgacgta gtccggttta
     4141 ccggtttttt tattcagcgc atagtgagtg tattgcccgg taagcggtaa tgcatcgata
     4201 cctttaaaaa agccgcccgc accttcggca taattgcgtg cagcagtaat ttccgagtcc
     4261 cccatcccat cgccaatcag caaaataata tttttggcgg gtttatcgct aagagaatca
     4321 cgcagagcgg cggtctgatc ccccgttaaa cggcgagcac cgccgggtgc agtaatatcg
     4381 ccctgagcag cccggttttc cagaacaggc atttctggtg tccgggcttt tgtcacaggg
     4441 gaaaacagta acggtaagag tgccagtgca atagtgcttt gtttcacttt attttctcca
     4501 tgtacaaata cattaaaaat aaaaacaaag cgactataag tctcggccgt gacagcttta
     4561 tgacagctgt tgaaaagatt aactttttac tgacgaggat gcttcaataa cttctttaca
     4621 taattgcgca gcagctccgt atcgtcgtca ggaatgctgg catcgggctt tacctcgtac
     4681 agcgccccct ctacctgatc aatcaacctc tgttggtcat tttgcgccat attgcgaagc
     4741 attgcagtga cgataatctc caaagcttct acctgcgcgc acagttcttt cgactcttct
     4801 tctttttggg caagcttaaa taacaactca gcaatgagat ttttcatgtc tgtatttcct
     4861 tatccaaagt atggagaagt taacattatg tttcacaact tcatagtgtc tggaagtatt
     4921 atttttatga caaaggaaat actttgcgca aatatttttt gtttgattac ca
//
LOCUS       DAZFLD010000266         4874 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.268, whole genome shotgun sequence.
ACCESSION   DAZFLD010000266 DAZFLD010000000
VERSION     DAZFLD010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4874
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.268"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            328..498
                     /locus_tag="ACHKBL_004416"
                     /pseudo
     CDS             328..498
                     /locus_tag="ACHKBL_004416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000526115.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(714..1547)
                     /locus_tag="ACHKBL_004417"
     CDS             complement(714..1547)
                     /locus_tag="ACHKBL_004417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFO5821183.1"
                     /translation="MMKLGFNEATCLRNSSLEKDLVLAERFGYDYIEIRLDMLQTWLQ
                     EHTLVELADIFSVSHLKPWGYNSLEDITFCDAGRWSEIRQQLVLACYAGEVVGGDCLV
                     VVPTIREGAHFSDRDTVRDSVKRLREMSALAAESQMRLAFEPIGSAGCCVRSLAMAME
                     IVDAVDRSNVGLAIDAFNLYLYDQWQDLSTLRQVPVEKIFVYHIDDADRLPVAVLDHC
                     HRLFPGNGAIPLYMITHELVKKGYTGICSLELFNPGYWQMAASEVFAIGAEKTRPFLA
                     D"
     gene            complement(1554..2891)
                     /locus_tag="ACHKBL_004418"
     CDS             complement(1554..2891)
                     /locus_tag="ACHKBL_004418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 30 beta sandwich
                     domain-containing protein"
                     /protein_id="HFO5821184.1"
                     /translation="MKGRLITSDPWNQQFMVELEVFFSPRQPACSELISVFPNHSLQQ
                     IDGFGGSFTESAGVVFNSMSDKTKAQFLNLYFSPQEHNYSLGRMPIQSCDFSLSNYAY
                     VDSSEDLRQGRISFSRDESHLMPLISAALRINPQIKLMASPWSPPAFMKTNYDMNGGG
                     KLRYEYYADWADIIIDYLLEYRRHGISVKALSVQNEPVAIKSWDSCLYSVEEETTFAV
                     HYLRPRLARYGMNEIEIYIWDHDKDGLIDWAERAFADEKDGDSINGMAFHWYTGDHFS
                     QIQYLARKLPNKKLLFSEGCVPMENDEGGQIRHWHTYIHDMIGNFKSGCSGFIDWNLL
                     LDSDGGPNHQSNLCEAPVQYDALNDILWCNHSWYGIGHFCRFVHPGAKVMLTSSYDTQ
                     LEDVGFVNPDGERVLVVYNRDTRERHFRVVDGDRELALTLPPSGASTLVWRQE"
     gene            complement(2989..3999)
                     /gene="iolG1"
                     /locus_tag="ACHKBL_004419"
     CDS             complement(2989..3999)
                     /gene="iolG1"
                     /locus_tag="ACHKBL_004419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol 2-dehydrogenase IolG1"
                     /protein_id="HFO5821185.1"
                     /translation="MTLKAGIVGIGKIGADHLRRLANTISGVEVVAVCDIVEGRAQAA
                     LDKYEIKAKDYKDYHDLINDEDVEVVVITASNEVHADVAVAALNANKYVFCEKPLAVT
                     ADDCQRVIGAERKNGKRMVQVGFMRRYDKGYVQLKNIIDSGEIGQPLMVHGRHYNVST
                     VSDYKTPQAIYETLIHEIDVMHWLLNEDYRTVKVYFPRQSSLVTTLRDPQVVVMETTS
                     GINIVVEVFVNCQYGYDIHCDVTCEKGMVELPTVATAAVRKEAKYSTDILVDWKQRFI
                     DAYDIEFQDFFDRLNAGLPLAGPTSWDGYLAAVTADACVKSQETGNTEFVELPAKPDF
                     YK"
     gene            complement(4017..>4874)
                     /gene="iolE"
                     /locus_tag="ACHKBL_004420"
     CDS             complement(4017..>4874)
                     /gene="iolE"
                     /locus_tag="ACHKBL_004420"
                     /EC_number="4.2.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463285.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050114 - myo-inosose-2 dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="myo-inosose-2 dehydratase"
                     /protein_id="HFO5821186.1"
                     /translation="WRNDDLPEIGKDNTYKQILSDAALTGFSGTEVGGCYPLDPTELN
                     IELALRHLEIPGKWFSSFIIRDGIESVKKAFEQHCAYLQAVHAYIAVVSEQTYSIQGI
                     IDKCVYTEKPYFNDIEWQQLCSGLNELGMIANTYGLKLAFHHHMGTGVQTLQEVDRLM
                     ENTDPQLVNLLFDTGHIYVSDGDVMSLLCKHFSRIKHVHFKDVRDEKLKACRAGKKSF
                     LNSFLDGMFTVPGDGNIDFKSVLAYLVENHYSGWIVVEAEQDPNIYNPLEYAQKGKKH
                     IDGLLGNYI"
BASE COUNT         1420 a         1204 c          935 g         1315 t
ORIGIN      
        1 tattaatcag ttaaattttc cgttactggg atgaggtgta cacttggcct ctcccaaaat
       61 ccgcggaacc gcccctggcc cctattcagc atttcaggct ctaagttgag gactgaccgg
      121 attgacatag ggcctgctga cgccattcat tgactaccaa cagatctttc ttgttattta
      181 tagaaaacca ccagccagcc tagtggttca gtaaaacttc caactttgag gctgttgtca
      241 aaaccccttt ttgatttgtt aaaacacctt gcagtctacc aactgtaagc gtcaaacaag
      301 aaatcaaaag gggattccaa tgggtgaatg aaaaagggct taacgaacac ccgatggaac
      361 tgtaaatatc acatagtttt tgcgccaaaa taccgaagac agatgttcta cagagaaaag
      421 cgaagtgcaa gagcaccagg caatattttg agaaaactgt gtgagtggaa aagtgtacgg
      481 attctggaag ctgaatgctg agcggatcat atccatatgc ttgtgaattt acggggcgta
      541 agtaaggaag ttgaatgaaa atgtcagatc atatgcgcct gtcagccagg gcgaggcaga
      601 aaacgaatct ttaagacaca tctgaaaaac ctccggctat gccggaggag atttattctg
      661 atcacctgct tattaccggg cacagcgaca gttcatttgc agttacgtca gcgttaatcc
      721 gctaagaaag gtcgcgtttt ttccgctccg atagcaaaga cctcgctggc tgccatttgc
      781 cagtaacctg ggttaaacaa ctccagtgaa cagatccctg tataaccttt tttcaccagt
      841 tcatgggtga tcatgtacag tggtatcgca ccgttgcctg gaaacagacg atgacaatga
      901 tccaacacag ctacaggtaa acggtcagca tcatcaatat gatagacaaa gatcttttcc
      961 accggaacct gtcgcagcgt actcagatcc tgccattgat cgtacagata aaggttaaag
     1021 gcatctattg ccagaccaac gttactccga tcaactgcgt ccacgatctc catcgccatc
     1081 gccagactgc gtacgcaaca tcccgcagag ccgataggct caaaggccag tcgcatctgg
     1141 ctctctgccg ctaatgctga catctcacgc aaacgtttca ctgaatctct taccgtgtca
     1201 cgatctgaaa aatgagcacc ttcacgaatg gtcggcacta caaccaggca atcaccacct
     1261 acgacttccc ctgcataaca ggctaaaacc agttgttgcc gtatttccga ccagcgcccg
     1321 gcatcacaaa atgtgatgtc ttccagcgag ttgtagcccc atggcttaag atggcttaca
     1381 gaaaaaatat cggccagttc aaccagagtg tgttcctgca gccacgtctg caacatatcc
     1441 agacggatct caatatagtc atatccaaaa cgttccgcta ggaccagatc cttctccagg
     1501 ctggaattac gcagacaggt cgcttcattg aatcccagtt tcatcattca gacttactcc
     1561 tgccgccata ccaatgtact ggctccagaa ggcggcagcg ttaatgcgag ttctctgtcg
     1621 ccatcgacca cccggaaatg cctttctcgc gtatcgcgat tatataccac taaaactcgc
     1681 tcaccatcag gattaacaaa acccacatct tccagttgcg tatcataact ggaagtaagc
     1741 ataactttcg caccgggatg cacaaagcgg caaaagtgtc caattccgta ccaagagtgg
     1801 ttacaccaga gaatgtcatt caatgcatcg tactgcacag gagcttcgca caaattactc
     1861 tggtgatttg ggcctccatc actgtctaac agcaggttcc agtcgataaa tccgctacag
     1921 ccggatttaa aattaccaat catgtcatga atataggtat gccagtgacg gatctggcca
     1981 ccttcatcat tctccattgg tacacatcct tcggaaaaca gcagcttttt attcggcaac
     2041 ttccgggcca gatactgaat ttgcgaaaaa tggtcaccgg tataccagtg gaaagccatc
     2101 ccgttgatgc tatcgccatc cttttcgtca gcaaaggcac gttcagccca gtcaatcaga
     2161 ccatctttat catgatccca gatatagatc tctatttcat tcattccata gcgggcgagt
     2221 cgtgggcgca gatagtgaac agcaaaagta gtctcttctt ctacactata cagacaggaa
     2281 tcccagcttt ttatcgcaac tggttcattc tgcactgata gtgctttaac actgatacca
     2341 tggcgtctgt attcgagcag atagtcgata ataatatctg cccagtcagc atagtattca
     2401 taccgcaatt tcccacctcc gttcatatca taattagtct tcatgaatgc cggtggactc
     2461 cacggggaag ccattaattt tatttgtgga ttgatccgca atgccgcgga aattagcggc
     2521 attaaatgag attcatcacg ggaaaaggaa atccttccct gacgcaggtc ttcactggaa
     2581 tcaacatagg cataattgct cagagagaaa tcacagcttt ggatcggcat ccgccccaaa
     2641 ctatagttat gttcctgagg cgaaaaatag agattcaaaa attgcgcctt tgtcttatca
     2701 ctcatgctgt tgaaaacaac accagcactt tcagtgaagc tgccgccaaa tccatcaatc
     2761 tgttgtaacg agtggtttgg aaagacgctg attaattcac tacacgctgg ttgacgaggc
     2821 gaaaaaaaaa cctccagctc aaccataaat tgctgattcc agggatcgga agtgattagt
     2881 ctgcctttca tagagggatt ccagataata taggatgata aattgtgata taccggacag
     2941 gagcatacac ctagctatcc ggtatgaaat tatcacatcg cagaaagact atttgtagaa
     3001 gtcaggtttt gctggtaatt ccacaaactc ggtgtttcct gtttcctggg acttcacaca
     3061 ggcatcagcc gttaccgcag ccagataacc atcccatgat gttgggcctg ccagaggtaa
     3121 tcccgcattc agacggtcaa agaagtcctg gaactcaata tcataggcat caataaagcg
     3181 ttgtttccag tccaccaaaa tgtcagtact gtattttgcc tctttacgca ccgcagcagt
     3241 agccacggta ggtaattcca ccatcccttt ctcacaggtc acatcacaat gaatatcata
     3301 gccatactgg caattcacaa acacctcaac cactatatta ataccggagg tcgtttccat
     3361 caccaccacc tgcggatctc gtagagtagt caccaagctg gactgacgtg ggaagtagac
     3421 cttaactgtt ctataatctt cgttgagtaa ccagtgcata acatcgattt catgaatcag
     3481 cgtttcataa attgcttgtg gtgttttgta atccgatacc gtgcttacat tataatgacg
     3541 accatgaacc attaatggtt gaccaatttc tccgctatcg atgatgtttt tcagctgtac
     3601 gtagccttta tcgtagcgac gcataaaccc aacttgcacc attcgttttc catttttacg
     3661 ttcagctccg atcacacgct gacaatcatc tgctgtaacc gcaagcggct tttcacagaa
     3721 aacatattta ttggcattta acgctgcaac ggctacatcg gcatgtactt cattcgaggc
     3781 agtaattacc actacttcga catcttcatc attaatcaga tcgtgatagt ctttatagtc
     3841 ttttgctttg atctcatact tatccaaagc tgcttgcgct ctgccttcga caatatcgca
     3901 cacagcaaca acttctactc cagaaatagt gttagccaga cgtcttaagt gatcggcacc
     3961 aattttcccg atccccacaa tacctgcttt taaagtcatc ttattttcct tttctatcag
     4021 atataattac ccaataaacc atcaatatgc tttttacctt tttgtgcata ttccaaagga
     4081 ttatatatat tcgggtcctg ctcagcttca acgacaatcc agccggaata atgattctca
     4141 accaaataag ccaatacaga tttaaaatca atattcccat caccaggaac agtaaacatt
     4201 ccatcaagaa aagaattgag aaatgatttt tttccagccc gacatgcttt tagtttttca
     4261 tcccgaacat ctttaaagtg aacatgttta atacgggaaa aatgcttgca caacagtgac
     4321 atcacatctc catcagaaac ataaatatga ccagtatcaa acagcaaatt cactaattgt
     4381 ggatctgtgt tttccataag tcgatccact tcctgtaaag tttgtacgcc agtgcccata
     4441 tgatggtgga aagcaagttt aagcccatat gtattggcga tcatgcctaa ctcgttcaat
     4501 cctgagcata attgttgcca ttcgatatca ttgaagtaag gtttttccgt atagacacat
     4561 ttatcaataa ttccttgaat actatacgtt tgctcagaca caacagcgat ataagcatgg
     4621 acagcctgca aataagcgca atgttgttca aaagctttct tcactgattc aataccatca
     4681 cgaataataa aagatgagaa ccactttccg ggaatttcca gatggcgaag tgctaactct
     4741 atatttaatt cagtcgggtc cagaggataa catcccccca cttctgttcc agaaaaacca
     4801 gtaagtgctg catcacttaa tatctgctta taggtattgt ctttaccaat ttcaggcaaa
     4861 tcatcatttc gcca
//
LOCUS       DAZFLD010000267         4828 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.269, whole genome shotgun sequence.
ACCESSION   DAZFLD010000267 DAZFLD010000000
VERSION     DAZFLD010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4828)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4828)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4828
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.269"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            9..3974
                     /locus_tag="ACHKBL_004421"
     CDS             9..3974
                     /locus_tag="ACHKBL_004421"
                     /inference="COORDINATES: protein motif:HMM:NF014452.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S6 family peptidase"
                     /protein_id="HFO5821187.1"
                     /translation="MTLSALPGKASTVSAEIPYQTFRDFAENKGVFTPGVTGIEIKDN
                     NGNAVGTLDVPMIDFSSVSRRGSLTLLSQGYGVSAKHGDLGDVNNASFGYDKNNYTVV
                     KNNKHSGLDFSLHRFSKLITEAAPADINIFGQLSDSSQYTAFYRAGAGTQYIKERSGK
                     QTHIPGTFLTGGTVGTPWYSGNNLISSSPGDTYNKSQGPLASYGQMGDSGSPLFAYDS
                     LSEKWSLAGVTLHNYGVNGYRNNWLLLPEDYIRNIIVADFDPIISFNKNSKEHMSWTY
                     DAAKGVGRIQQDDQQFVMHGNLNGNLNAGKNLYFTGENGIIDLKDNVNQGAGYLQFAD
                     DYTVTTSNDSSWSGGGIIVNYGTTVKWGINGVFGDDLHKVGDGTLIINGTGKNEGGLK
                     IGAGTVILEQKAKNNDSTAFSSINISGGNSRVKLSGDNQIIPDNVSWGFRGGYLDING
                     KDTEFSRLQAVDYGAAIINSSIDKSLLTLNLSPLKKDEIAVSVKVLDMNAIFQGGHGT
                     AGDLYKTTFYGPTQYYLLKKPKFGSVLMGALKNTSEWQFAGTDLNQAVDMAKNNKLTS
                     SAQASYLYHGKLLGNMDIVIPELTGNDILTLDGSVSISGDMSKQDGALIFQGHPVIHA
                     GQTVSASQSDWENREFSLNNLNLNNADFSLSRNAFMNGNIRAVNQSTVIIGGDTVFTD
                     KNDGTGNDVISVEGKSAAAGTSSYTGHITLEQKSALDIRDNFRGGVTSEDSHINVSSS
                     SVLFSDASSFINSSLNIHKGGALTAQGGLFTSGSIDIGDASLLLTGTPVNSDDAAFLP
                     TINMADGGFKLMSDSSVLKARDQASVVGDIISDKQATISFGTESGKEGILSEKASRGL
                     AVGLLSGFNTAYRGAIHAPSASATVNNTWWQLTGDSSLRSLKNTGSMTYFTGSAANKA
                     FHTLTVDELTTNGTAYAMRTDLKNADKLVVNQKLSGKDNILLVDFLNKPTGEKLDIEL
                     VSAPGNSSKDVFKGSEQAIGFSNVTPVITAIDAGDKTTWNLTGYRMAENPAATQSASG
                     LASVGYKSFLSEVNNLNKRMGDLRDINGEAGAWARIMSGTGSAGGGFSDNHTHVQVGV
                     DKKHELDGLDLFTGFTVTHTDSSASADAFKGKTKSVGAGLYASAMFDSGAYIDLIGKY
                     VHHDNEYTATFAGLGTRDYSTHSWYAGAEAGYRCHVTEDTWIEPQAELVYGAVSGKQF
                     AWKDQGMHLSMKDRDYNPLIGRTGVDVGKSFSGKDWKVTARAGLGYQFDLLANGETVL
                     RDASGEKRIKGEKDSRMLMSVGLNAEIRDNVRFGLEFEKSAFGKYNVDNAVNANFRYS
                     F"
     gene            4507..4827
                     /locus_tag="ACHKBL_004422"
                     /pseudo
     CDS             4507..4827
                     /locus_tag="ACHKBL_004422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997724.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1397 a         1028 c         1186 g         1217 t
ORIGIN      
        1 tattatcagt gactctttct gctttaccgg gtaaagcatc aacggtcagc gcagaaatac
       61 catatcagac ttttcgtgac tttgctgaaa ataaaggtgt gtttaccccc ggagtgacag
      121 gaattgaaat aaaagataat aatggaaatg cagttggtac gcttgatgtg ccaatgattg
      181 acttcagcag cgtgtcacgg cggggctcac tgaccctgtt gtcgcaggga tatggtgtat
      241 cagcaaaaca cggagacctt ggtgatgtaa acaatgccag ttttggctat gataaaaata
      301 attatactgt agtaaagaac aacaaacatt cgggccttga tttttctctg catcggttca
      361 gcaaactcat cacagaggct gcacctgctg atataaatat attcggacaa cttagtgatt
      421 cgtcacagta tactgctttt tacagagcag gcgctggcac acagtatatt aaagaacgct
      481 caggtaaaca gactcatatc ccgggaacat ttttaactgg tggaacagta ggcactccat
      541 ggtattcagg aaataattta atatcaagct ctccaggtga cacctataat aaatcacagg
      601 gaccattagc cagttatggg cagatgggtg acagcgggtc gccattattt gcttatgatt
      661 ctctcagtga aaaatggtct ctggctggag tcaccctgca taactatggg gtgaacggtt
      721 acagaaataa ctggttgtta ttacctgagg attatatcag aaatattatt gtagccgatt
      781 tcgatcccat tatttcattt aataaaaaca gtaaagaaca tatgtcatgg acatatgatg
      841 cagccaaagg cgtgggccgt attcagcagg atgaccaaca gtttgtgatg catggtaacc
      901 tgaatggtaa tctgaatgca ggtaaaaatc tttattttac aggcgaaaat ggcattattg
      961 atttaaaaga taatgttaat cagggtgccg gatatcttca gtttgctgat gattatacag
     1021 taacaacgag caatgacagt tcatggtccg gaggaggcat tattgtcaat tatggtacaa
     1081 ctgtaaaatg gggtataaac ggggttttcg gagatgacct tcataaagtt ggggacggaa
     1141 cgcttattat taatggaacc gggaaaaatg aagggggact aaagattggg gctggtactg
     1201 taatccttga acaaaaagcg aaaaacaacg attcaacggc tttttcttcc attaacatca
     1261 gtgggggaaa ctcgcgcgtt aaactttcag gagataatca gattatacct gataatgttt
     1321 cctggggatt ccggggggga tatttagata taaacggaaa agacactgag ttctcccggt
     1381 tacaggctgt tgattatggg gctgcaatta ttaacagcag catagacaag tcattattga
     1441 ctttaaatct gtcaccattg aaaaaagatg aaatagctgt aagcgtgaag gtgttagata
     1501 tgaacgccat ctttcagggg ggacacggga cagccggaga tttgtataaa acaacttttt
     1561 acggcccgac acagtattat ctgctgaaaa aacctaaatt tggcagtgtg ctgatggggg
     1621 cactgaaaaa caccagcgaa tggcaatttg caggaacaga cctgaatcag gccgttgata
     1681 tggcaaagaa taataaactg acatcaagtg cacaggcttc atatctttat cacggtaagc
     1741 tcctggggaa tatggatatt gtcattccgg agcttacagg caatgatatt ttaacgcttg
     1801 atggctctgt cagtatatca ggagatatgt caaaacagga cggtgctctt atcttccagg
     1861 gacacccggt tattcatgca gggcaaactg tttctgcatc gcagagtgac tgggagaaca
     1921 gggagttctc actcaacaat ctgaatctta ataatgcgga cttcagtctg tcccgtaatg
     1981 catttatgaa cgggaatatc agggccgtta accagagcac tgttattatc ggcggagata
     2041 cagtctttac tgataaaaat gacggaacag gtaatgatgt catcagtgtt gaagggaaat
     2101 ctgctgccgc aggaacatcc tcctatacag ggcatatcac tctggagcaa aaatcagcac
     2161 tggatatccg cgataatttt cgtggcgggg ttacgtctga agacagtcat atcaatgttt
     2221 cttcatcttc agtcctgttc tcagatgcat cgtcatttat aaacagctcc ctgaatattc
     2281 ataaaggagg tgcgctgacc gctcagggag ggctgtttac aagtggaagc attgatattg
     2341 gtgacgcttc ccttctgctt accggtacac cagtgaattc agatgatgct gcttttttac
     2401 cgaccatcaa tatggctgat ggcggattta aactgatgtc tgattcatca gtactgaaag
     2461 ccagagacca ggcatctgtt gttggtgata ttatttctga taaacaggcc acaatcagct
     2521 tcggaactga atcaggtaaa gagggcatat tgtctgagaa ggcatcccgg ggactcgcgg
     2581 taggattact gagtggtttt aatacggcat accgcggtgc aattcatgcc ccgtcagcat
     2641 ctgccactgt gaacaacacc tggtggcaat tgacaggaga ctcctcactt cgctcgttaa
     2701 aaaataccgg aagcatgaca tattttacag gaagtgcagc gaataaagca ttccatacac
     2761 tgacggttga tgagctgacg acgaatggca ctgcgtatgc catgcgtacg gacctgaaaa
     2821 atgcggataa gctggtagta aaccaaaagc tgtcaggtaa ggacaatatt ctgctggttg
     2881 attttctgaa caaacccacc ggagaaaaac tggatattga actggtgagt gcaccgggga
     2941 acagcagtaa ggatgttttt aaaggaagtg aacaggcaat aggtttcagc aatgtcacgc
     3001 ctgttattac agctatagac gccggagata aaacaacatg gaacctgacc gggtacagga
     3061 tggcagaaaa tcctgccgca acccaaagtg cctcaggcct tgcatctgtg gggtacaaat
     3121 catttttgag tgaggtcaac aacctgaata aacgtatggg tgacctgcgt gacatcaatg
     3181 gtgaagctgg cgcatgggca cgtatcatga gcggaaccgg ctctgccggt ggtggtttca
     3241 gtgacaacca cacacatgtt caggtcggtg tcgacaaaaa acatgagctg gacggactgg
     3301 atttgtttac cggcttcact gtcacacata ctgacagcag tgcctctgct gatgctttca
     3361 aaggtaaaac aaaatctgtg ggggccggac tctatgcttc cgccatgttt gattccggtg
     3421 cctatatcga cctgattggt aagtatgttc atcatgataa tgagtacacc gcaacctttg
     3481 ccggactcgg aacccgtgat tacagtacgc attcatggta tgccggtgct gaagcaggct
     3541 accgctgtca tgtcactgag gatacctgga ttgagccaca ggcagaactg gtttacggtg
     3601 ctgtatccgg taaacagttt gcatggaagg accaggggat gcatctgtcc atgaaggaca
     3661 gggactacaa tccgctgatt ggtcgtaccg gtgtggatgt gggtaaatcc ttctcaggta
     3721 aggactggaa agtgacagcc cgtgccggtc tgggctacca gttcgacctg ctggctaacg
     3781 gcgaaaccgt attgcgggac gcatcaggtg aaaaacgtat caaaggtgaa aaagacagcc
     3841 gtatgctgat gtccgttggc ctgaatgcag aaatcaggga caacgtccgc tttggactgg
     3901 agtttgagaa atccgccttt ggtaagtaca acgttgataa tgctgtcaac gctaacttcc
     3961 gttactcgtt ctgatactcc gtattcaaaa gggcctgagg gccctttatg ctcaggacct
     4021 gccgcccggg tatacatttc cggagtatca gtcgatttgt ttacttaacc gccatgacca
     4081 aagccggcag taatgtcggc tttttacgtc catatattcc actcatccgg aactgtgaca
     4141 cagaacctga atacaggatt gatcaccggt gcccacaaat ggcacatttt cagtaagatt
     4201 ctgaatgtta cattccccct tcatgtgtga cacagctgtc acagagaacg aaacggaaag
     4261 cacatgaaaa acataaaaac tcacaccgga ctccttatcc acaaagaaca aacacggcgc
     4321 gttcgtcttc atgaaacacc ggccgcctgg tgccacacac gccgggaatg ctacagcaaa
     4381 acgaccggtc gtcgctgcgg cagccctgac agtctcagca ggctgattct gagcagcatt
     4441 cagcccatag aatgagataa cccgtatgaa tgatgacaga cgtctacggt acccgatgta
     4501 tggaacattt gctttctggc taacggcatg cccgacacat ggttttacac cgatgacgcc
     4561 tccgtcacaa aagagtgggg gcgggaatat tgtgacaatg tggaagtgtt ccctctgtgc
     4621 tatatgcatg acggtcccgt tttccggggg cgctatattg atgagctcag cgccgcacgg
     4681 aacagaatgc tccatatgca ccacatactg ccggaatgaa cccggtacag agaaaatttt
     4741 catgacgcag aatgtatccg tgaaattcac gaagcgcccc tgcaggctcc cggcattctg
     4801 caaaaataca aacgcacaaa atcataag
//
LOCUS       DAZFLD010000268         4799 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.270, whole genome shotgun sequence.
ACCESSION   DAZFLD010000268 DAZFLD010000000
VERSION     DAZFLD010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4799
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.270"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            139..1101
                     /gene="tauA"
                     /locus_tag="ACHKBL_004423"
     CDS             139..1101
                     /gene="tauA"
                     /locus_tag="ACHKBL_004423"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO5821188.1"
                     /translation="MAISSRNTLLAALAFIAFQAQAVNVTVAYQTSAEPAKVAQADNT
                     FAKESGASVDWRKFDSGASIVRALASGDVQIGNLGSSPLAVAASQQVPIEVFLLASKL
                     GNSEALVVKKTISKPEDLIGKRIAVPFISTTHYSLLAALKHWGIKPGQVEIVNLQPPA
                     IIAAWQRGDIDGAYVWAPAVNALEKDGKVLTDSEQVGQWGAPTLDVWVVRKDFAEKHP
                     EVVKAFAKSAIDAQQPYIANPDAWLKQPENISKLARLSGVPEGDVPGLVKGNTYLTPQ
                     QQTAELTGPVNKAIIDTAQFLKEQGKVPAVANDYSQYVTSRFVQ"
     gene            1114..1881
                     /gene="tauB"
                     /locus_tag="ACHKBL_004424"
     CDS             1114..1881
                     /gene="tauB"
                     /locus_tag="ACHKBL_004424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter ATP-binding subunit"
                     /protein_id="HFO5821189.1"
                     /translation="MLQISHLYADYGGKPALEDINLTLESGELLVVLGPSGCGKTTLL
                     NLIAGFVPYQHGSIQLAGKCIEGPGAERGVVFQNEGLLPWRNVVDNVVFGLQLAGVEK
                     MQRLEIAHQMVKKVGLEGAEKRYIWQLSGGQRQRVGIARALAANPQLLLLDEPFGALD
                     AFTRDQMQTLLLKLWQETGKQVLLITHDIEEAVFMATELVLLSPGPGRVLERLPLNFA
                     RRFVAGESSRSIKSDPQFIAMREYVLSRVFEQREAFS"
     gene            1878..2705
                     /gene="tauC"
                     /locus_tag="ACHKBL_004425"
     CDS             1878..2705
                     /gene="tauC"
                     /locus_tag="ACHKBL_004425"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter permease TauC"
                     /protein_id="HFO5821190.1"
                     /translation="MSVLINEKLHSHRLKWRWPLSRQVTFSIGTLAVLLTVWWAVAAL
                     QLISPLFLPPPQQVLAKLLTIAGPQGFMDATLWQHLAASLTRILLALLAAVLIGIPVG
                     IAMGLSPTVRGILDPIIELYRPVPPLAYLPLMVIWFGIGETSKILLIYLAIFAPVAMS
                     ALAGVKSAQQVRIRAAQSLGASRAQVLWFVILPGALPEILTGLRIGLGVGWSTLVAAE
                     LIAATRGLGFMVQSAGEFLATDVVLAGIAVIAIIAFLLELGLRALQRRLTPWHGEVQ"
     gene            2702..3553
                     /gene="tauD"
                     /locus_tag="ACHKBL_004426"
     CDS             2702..3553
                     /gene="tauD"
                     /locus_tag="ACHKBL_004426"
                     /EC_number="1.14.11.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine dioxygenase"
                     /protein_id="HFO5821191.1"
                     /translation="MSERLSITPLGPYIGAQITGADLTRPLSDNQFEQLYHAVLRHQV
                     VFLRDQAITPQQQRALAQRFGELHIHPVYPHAEGVDEIIVLDTHNDNPPDNDNWHTDV
                     TFIQTPPAGAILAAKELPSTGGDTLWASGIAAYEALSVPFRQLLSGLRAEHDFRKSFP
                     EYKYRKTEAEHQRWREAVAKNPPLLHPVVRTHPVTGKQALFVNEGFTTRIVDVSEKES
                     EALLGFLFAHITKPEFQVRWCWQPNDIAIWDNRVTQHYANADYLPQRRIMHRATILGD
                     KPFYRAG"
     gene            complement(3593..4567)
                     /gene="hemB"
                     /locus_tag="ACHKBL_004427"
     CDS             complement(3593..4567)
                     /gene="hemB"
                     /locus_tag="ACHKBL_004427"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HFO5821192.1"
                     /translation="MTDLIQRPRRLRKSPALRAMFEETTLSLNDLVLPIFVEEEIDDY
                     KAVEAMPGVMRIPEKHLAREIERIANAGIRSVMTFGISHHTDETGSDAWREDGLVARM
                     SRICKQTVPEMIVMSDTCFCEYTSHGHCGVLCEHGVDNDATLENLGKQAVVAAAAGAD
                     FIAPSAAMDGQVQAIRQALDAAGFKDTAIMSYSTKFASSFYGPFREAAGSALKGDRKS
                     YQMNPMNRREAIRESLLDEAQGADCLMVKPAGAYLDIVRELRERTELPIGAYQVSGEY
                     AMIKFAALAGAIDEEKVVLESLGSIKRAGADLIFSYFALDLAEKKILR"
BASE COUNT         1078 a         1226 c         1383 g         1112 t
ORIGIN      
        1 tgtaattgca tatttaatct ttcctttgcc actttttcgc taagaataaa atcatctgtg
       61 cgataacaac taattctttt aatgaatgtt tttattcctg aatactgctc ccataacaag
      121 acaggggagc agacaatcat ggcaatttca tcgcgtaaca cacttcttgc cgcactggca
      181 ttcatcgctt ttcaggcaca ggcggtgaac gtcactgtgg cgtatcaaac ttcagccgaa
      241 ccggcgaaag tggctcaggc cgacaacacc tttgctaaag agagcggggc gagcgttgac
      301 tggcgtaagt ttgacagcgg agccagcatc gtgcgggcgc tggcttctgg cgacgtgcaa
      361 atcggtaacc tcggttccag cccattagcg gtggcagcca gccaacaggt gccgattgaa
      421 gtcttcttac tggcgtcaaa actgggtaac tccgaagcgc tggtggtgaa gaaaactatc
      481 agcaaaccgg aagatctgat tggcaagcgc atcgccgtac cgtttatctc caccacccac
      541 tacagcctgc tggcggcgct gaaacactgg ggtattaaac ctgggcaagt ggagattgtg
      601 aacctgcagc cgcccgcaat tatcgccgcc tggcagcggg gagatattga tggtgcttat
      661 gtctgggcac cggcggttaa cgcgctggaa aaagacggca aggtgctgac cgattctgaa
      721 caggtcgggc agtggggcgc gccgacgctg gacgtctggg tcgtgcggaa agattttgcc
      781 gagaaacatc ctgaggtcgt gaaagcgttc gctaaaagcg ccatcgatgc tcagcaaccg
      841 tacattgcta acccagacgc gtggctgaaa cagccggaaa acatcagcaa actggcgcgg
      901 ttaagcggcg tgcctgaagg tgacgttccg gggctggtga aggggaatac ctatctgacg
      961 ccgcagcagc aaacggcaga actgaccgga ccggtgaaca aagcgatcat cgacaccgcg
     1021 cagtttttga aagagcaggg caaagtcccc gccgtggcga atgattacag ccagtacgtt
     1081 acctcgcgct tcgtgcaata aaaggaggcg cagatgctgc aaatttctca tctttacgcc
     1141 gattatggcg gcaaaccggc actggaagat atcaacctga cgctggaaag cggcgagctt
     1201 ctggtagttc tggggccgtc tggctgcggc aaaaccacct tgctgaatct gattgccggt
     1261 tttgtgcctt atcagcatgg cagcattcaa ctggcgggta agtgcattga gggaccggga
     1321 gcagagcggg gcgtggtttt tcagaatgaa gggctgttgc cgtggcgtaa tgtggtagac
     1381 aacgtggtgt tcggcctgca actggcaggt gtagagaaaa tgcagcgact ggaaatcgca
     1441 caccagatgg tgaaaaaagt ggggctggaa ggcgcagaaa aacgctacat ctggcagctt
     1501 tccggtggtc aacgtcagcg ggtggggatt gcccgcgcgt tggcggcaaa tccccagctg
     1561 ttattactcg acgaaccgtt tggtgcgctg gacgccttca cccgcgacca gatgcaaacc
     1621 ctgctgctga aactctggca ggagacggga aagcaggtgc tgttgattac ccacgatata
     1681 gaagaagcgg tgtttatggc gactgaactg gttctgcttt cacccggccc aggccgtgtg
     1741 ctggagcggc tgccgctcaa ctttgctcgc cgctttgttg cgggagagtc gagccgcagc
     1801 atcaagtccg atccacaatt catcgccatg cgcgaatatg ttttaagccg cgtatttgag
     1861 caacgggagg cgttctcatg agtgtgctca ttaatgaaaa actgcattcg catcggctga
     1921 aatggcgctg gccgctctcg cgtcaggtga ccttcagcat tggtacgtta gcggttttgc
     1981 tcactgtatg gtgggcagtc gcggcgctgc aactgattag cccgctgttt ttaccgccac
     2041 cgcaacaggt actggcaaaa ctgctcacca ttgccgggcc gcagggattt atggacgcca
     2101 cgctgtggca gcatctggca gccagcctga cgcgcatttt gctggcgcta ctcgcggcgg
     2161 tgttgatcgg tattccggtc gggatcgcca tggggctaag cccgacggtg cgcggcattc
     2221 tggatccgat aatcgagctt tatcgtccgg tgccgccgct ggcttatttg ccgctgatgg
     2281 tgatctggtt tggtattggc gaaacctcga agatcttact gatctattta gcgatttttg
     2341 cgccggtggc gatgtcggcg ctggcggggg tgaaaagcgc gcagcaggtt cgcattcgtg
     2401 ccgcccagtc gctgggtgcc agccgtgcgc aggtgctgtg gtttgtcatt ttgcccggcg
     2461 cgctgccgga aatcctcacc ggattacgca ttggtctggg tgtgggctgg tctacgctgg
     2521 tggcggcgga gctgattgcc gcaacgcgcg gtttaggatt tatggttcag tcagcgggtg
     2581 aatttctcgc gactgacgtg gtgctggcgg ggatcgcggt gattgcgatt atcgcctttc
     2641 ttttagaact gggtctgcgc gcgttacagc gccgcctgac gccctggcat ggagaagtac
     2701 aatgagtgaa cgtctgagca ttaccccgct ggggccgtat attggcgcac aaattacggg
     2761 ggccgactta acgcgcccgt taagcgataa tcagtttgaa cagctttacc atgcggtgct
     2821 gcgccatcag gtggtgtttc tgcgcgatca agctattacg ccgcagcagc aacgcgcgct
     2881 ggcccagcgt tttggcgagc tgcatattca ccctgtttac cctcatgctg aaggggttga
     2941 cgagatcatc gtcctggata cccataacga taatccgccg gataacgaca actggcacac
     3001 cgatgtgaca tttattcaaa cgccacccgc aggggcgatt ctggcggcga aagagttacc
     3061 ctcgaccggc ggcgatacgc tctgggccag tggtattgcc gcatatgagg cgctctctgt
     3121 tcccttccgc cagctgttga gcgggttgcg tgcggagcat gatttccgta aatcgttccc
     3181 ggaatacaag taccgcaaaa ccgaggctga gcatcaacgc tggcgtgagg cggtcgcgaa
     3241 aaatccaccg ttgctacatc cggtggtgcg aacgcatccg gtgaccggta aacaggcgct
     3301 gtttgtgaat gaaggcttta ccacgcgaat tgttgatgtc agcgagaaag agagcgaagc
     3361 cttgctgggc tttttatttg cccacatcac caaaccggag tttcaggtgc gctggtgctg
     3421 gcaaccaaat gatattgcga tttgggataa ccgcgtaacc cagcactatg ccaatgcgga
     3481 ttacctgcca cagcggcgga taatgcatcg ggcgacgatt cttggggata aaccgtttta
     3541 tcgggcgggg taagggggaa atgccgggtt ggataacccg gcataatgat cattaacgca
     3601 gaatcttctt ctcagccaaa tccagcgcaa agtagctgaa aatcagatcc gcacccgcac
     3661 gcttaatcga acctaagctt tcgagcacga ctttctcttc atctatagca cccgccagcg
     3721 cggcgaactt aatcatcgcg tactcaccgc tcacctgata cgcgccaatc ggcaattcag
     3781 tacgttcacg cagctcacgc acgatgtcga ggtatgctcc ggcaggttta accatcaggc
     3841 agtctgcgcc ctgggcttca tccagcagtg actcacgaat cgcctcacga cggttcattg
     3901 ggttcatctg atagcttttg cggtcgcctt ttaatgcgct tccggcagct tcacggaacg
     3961 ggccataaaa ggaggaggcg aacttggtcg aatacgacat aatcgccgta tctttaaagc
     4021 ccgcagcgtc cagcgcctgg cgaatcgcct gtacctgacc atccatcgca gcggaagggg
     4081 cgatgaaatc tgcgcctgca gcagctgcaa ccacggcttg cttgcctaaa ttttccagag
     4141 tcgcgtcgtt gtcgacgcca tgctcgcaca gcacaccgca gtgaccgtga gaagtgtatt
     4201 cacagaaaca agtgtctgac atgacgatca tttctggcac agtctgcttg cagatgcgcg
     4261 acatacgcgc caccagtcca tcttcccgcc aggcgtcgct gccggtttca tcggtatggt
     4321 gagagatgcc gaaagtcatc acggaacgaa taccggcgtt ggcgatgcgt tcaatttcgc
     4381 gtgccagatg tttctctgga atgcgcatca cgcctggcat ggcttcaacg gctttgtagt
     4441 cgtcaatttc ttcttcaaca aagatcggca acaccaggtc gttaaggcta agtgttgtct
     4501 cttcaaacat agcgcgcagc gcaggagatt tgcgcaggcg acgagggcgt tggattaaat
     4561 ctgtcatggt ctgcctgatg tttgtggaat cgaggagcat agtatacctg aagcaggctg
     4621 ggtgtgtttt acgaaagttg tcgcgatgtt gacaagaaga gaatgaaaaa gaggtcagga
     4681 catctttgta atagaaatag caataatttc atattcactg aaaatatttt taatttttat
     4741 ttatagccat agattattgt ttttggggat ttggtgacgt tttcaaaaag tgaataact
//
LOCUS       DAZFLD010000269         4794 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.271, whole genome shotgun sequence.
ACCESSION   DAZFLD010000269 DAZFLD010000000
VERSION     DAZFLD010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4794
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.271"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(124..200)
                     /locus_tag="ACHKBL_004428"
     tRNA            complement(124..200)
                     /locus_tag="ACHKBL_004428"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(164..166),aa:Met,seq:cat)
     gene            409..1506
                     /gene="mltA"
                     /locus_tag="ACHKBL_004429"
     CDS             409..1506
                     /gene="mltA"
                     /locus_tag="ACHKBL_004429"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HFO5821193.1"
                     /translation="MKGRWVKYLLMGTVVAMLAACSSKPTDRGQQYKDGKFTQPFSLV
                     NQPDAVGAPINAGDFAEQINHIRNSSPRLYGNQSNVYNAVQEWLRAGGDTRNMRQFGI
                     DAWQMEGVDNYGNVQFTGYYTPVIQARHTRQGEFQYPIYRMPPKRGRLPSRAEIYAGA
                     LSDKYILAYSNSLMDNFIMDVQGSGYIDFGDGSPLNFFSYAGKNGHAYRSIGKVLIDR
                     GEVKKEDMSMQAIRHWGETHSEAEVRELLEQNPSFVFFKPQSFAPVKGASAVPLVGRA
                     SVASDRSIIPPGTTLLAEVPLLDNNGKFNGQYELRLMVALDVGGAIKGQHFDIYQGIG
                     PEAGHRAGWYNHYGRVWVLKTAPGAGNVFSG"
     gene            1583..2389
                     /gene="tcdA"
                     /locus_tag="ACHKBL_004430"
     CDS             1583..2389
                     /gene="tcdA"
                     /locus_tag="ACHKBL_004430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HFO5821194.1"
                     /translation="MSVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSW
                     AAEALARTGIGAITLIDMDDVCVTNTNRQIHALRDNVGLAKAEVMAERIRQINPECRV
                     TVVDDFVTPDNVAQYMNAGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQID
                     PTQIQVTDLAKTIQDPLAAKLRERLKSDFGVVKNSKGKLGVDCVFSTEALVYPQSDGT
                     VCAMKATAEGPKRMDCASGFGAATMVTATFGFVAVSHALKKMMAKAARQG"
     gene            complement(2440..2883)
                     /gene="csdE"
                     /locus_tag="ACHKBL_004431"
     CDS             complement(2440..2883)
                     /gene="csdE"
                     /locus_tag="ACHKBL_004431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase sulfur acceptor subunit
                     CsdE"
                     /protein_id="HFO5821195.1"
                     /translation="MTNPQFAGHPFGTTVTAETLRNTFAPLTQWEDKYRQLIMLGKQL
                     PALPEELKAQAKEIAGCENRVWLGYTVAENGKMHFFGDSEGRIVRGLLAVLLTAVEGK
                     TAAELQAQSPLALFDELGLRAQLSASRSQGLNALSEAIIAAAKQV"
     gene            complement(2883..4088)
                     /gene="csdA"
                     /locus_tag="ACHKBL_004432"
     CDS             complement(2883..4088)
                     /gene="csdA"
                     /locus_tag="ACHKBL_004432"
                     /EC_number="3.13.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase CsdA"
                     /protein_id="HFO5821196.1"
                     /translation="MNVFNPAQFRAQFPALQDAGVYLDSAATTLKPEAVVEATRQFYS
                     LSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNAPDDKTIVWTRGTTESINMVAQCYA
                     RPHLQPGDEIIVSVAEHHANLVPWLMVAQQTGAKVVKLPLNAQRLPDVDLLPELITPR
                     SRILALGQMSNVTGGCPDLARAITFAHSAGMVVMVDGAQGAVHFPADVQQLDIDFYAF
                     SGHKLYGPTGIGVLYGKSELLEAMSPWLGGGKMVHEVSFDGFTPQSAPWKLEAGTPNV
                     AGVIGLSAALEWLADYDINQAESWSRSLATLAEDALAKRPGFRSFRCQDSSLLAFDFA
                     GVHHSDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAV
                     DRALELLVD"
     gene            4280..4507
                     /gene="ygdI"
                     /locus_tag="ACHKBL_004433"
     CDS             4280..4507
                     /gene="ygdI"
                     /locus_tag="ACHKBL_004433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HFO5821197.1"
                     /translation="MKKTAAIISACMLTFALSACSGSNYVMHTNDGRTIVSDGKPQTD
                     NDTGMISYKDANGNKQQINRTDVKEMVELDQ"
BASE COUNT         1154 a         1204 c         1292 g         1144 t
ORIGIN      
        1 tgatggaggg atattaacgg tttactcatt gtctcaggct tcactgaaac gggtaaggaa
       61 gcgtaaaaac gaaaaaaccg caagccaata gtagctttgc ggtttgtcag cattctaata
      121 aattggttgc gggggccgga tttgaaccga cgaccttcgg gttatgagcc cgacgagcta
      181 ccaggctgct ccaccccgcg tccgtggatg cgcactatac tctgctcgcg ttttcatgca
      241 accttttttt taccccgatc acggattcgg catgattttg aacaaaaaca aatcactaaa
      301 gcctattttt tgtgcaaaat ttgcgtcagg gagaggctgc tcatttcatt aaacgcggtg
      361 gtttgttatc ttcgttgcgc cttatttttt aacctgaaga agagaacaat gaaaggacgt
      421 tgggtaaagt accttcttat gggcacggtt gtggcaatgc ttgccgcctg ctcttccaaa
      481 ccaaccgatc gcggacagca atataaagac gggaaattta cccagccttt ctctctggtg
      541 aaccagccag atgccgttgg cgcgccgatt aatgccggtg attttgccga gcaaattaac
      601 catatccgta attcgtcacc gcgtctgtat ggcaaccaga gtaatgttta taacgcggtg
      661 caagagtggc tgcgcgcagg cggtgatacc cgcaatatgc gccagttcgg cattgatgcc
      721 tggcagatgg aaggtgtcga caactatggt aacgtgcagt ttacaggcta ttacacgccg
      781 gtaattcagg cgcgccatac ccgtcagggc gagttccagt atccgattta tcgtatgccg
      841 ccaaaacgtg gtcgtctgcc gtctcgtgcg gagatctacg caggagcgct gagtgataaa
      901 tatattctcg cttacagtaa ctccctgatg gataacttca ttatggatgt gcagggtagc
      961 gggtatatcg actttggtga tggcagtccg cttaactttt tcagctatgc agggaaaaac
     1021 ggtcatgcct atcgcagcat tggtaaggtg ttgatcgacc gtggcgaagt gaaaaaagaa
     1081 gatatgtcga tgcaggcgat tcgtcactgg ggcgaaacac acagtgaagc ggaggttcgc
     1141 gagctgctgg aacagaaccc gtctttcgtc ttctttaaac cgcaatcttt tgcaccggtg
     1201 aaaggggcaa gtgcggtgcc gctggttggc cgtgcttctg ttgcctccga tcgttcaatt
     1261 attccgccag gtactacctt gctggcagaa gtgccgctgc tggataataa cggcaaattt
     1321 aacggtcagt acgaattgcg tctgatggtg gcgctggatg tcggtggcgc aatcaaaggc
     1381 caacacttcg atatctatca agggatcggg ccggaagccg gacaccgcgc aggttggtac
     1441 aaccactatg gacgtgtctg ggtgctgaaa accgccccgg gcgcaggtaa cgtctttagc
     1501 ggctgatgtg atattctgta tgccgttttt acggatatga cagggtgagg ggtaacctca
     1561 ccctttttaa ttctgaggtg ctatgtctgt ggtaattagt gatgcatggc gtcagcgttt
     1621 tggtggcaca gcgcgtctgt atggtgaaaa agcattgcaa ctgtttgctg acgcgcatat
     1681 ttgtgtggtt ggtatcggtg gtgtcggttc ctgggcggcg gaagcgctag cgcgcacggg
     1741 gattggcgca atcacgctta tcgatatgga tgatgtgtgc gtcaccaata ccaatcggca
     1801 aattcacgct ctgcgcgata acgtcgggct ggcaaaagcg gaagtaatgg cggagcgtat
     1861 tcgccagatt aacccggagt gccgtgtaac agtagtggat gacttcgtga cgccggataa
     1921 cgtagcgcaa tatatgaatg ccggttattc gtacgtgatt gatgctattg atagtgtacg
     1981 gcccaaagcg gcgctgattg cttattgtcg gcgcaataaa atcccgctgg tcacgaccgg
     2041 tggcgctggt gggcagattg acccgacgca gattcaggtt accgatctgg cgaaaacgat
     2101 tcaggacccg ttggcggcga agttgcgcga gcgcctgaaa agcgattttg gcgtagtgaa
     2161 aaacagtaaa ggtaagctcg gcgtggattg cgtgttctct accgaagcgc tggtgtaccc
     2221 gcagtcagac ggcaccgtct gtgcgatgaa agccacggcg gaagggccaa agcggatgga
     2281 ttgtgcctca ggatttggcg cggcgacgat ggtgaccgcc acttttggct ttgttgcggt
     2341 ttctcatgcg ctgaagaaga tgatggcgaa agcggcgcgt caggggtaaa caatcaggtc
     2401 ggatgcgacg cgaacgtctt atccgacctg gttctgtgct taaacctgct tcgccgcagc
     2461 gataatcgcc tcgcttaacg cattcaaccc ctggctgcgt gaggcgctaa gttgcgcacg
     2521 taatcccagc tcatcaaaca atgccagcgg cgactgtgcc tgcaattcgg cggcggtttt
     2581 cccctcaacg gcagtcaaca acaccgccaa caggccgcgc acaatgcgcc cttcgctgtc
     2641 gccaaagaaa tgcatcttgc cgttttcagc cactgtatat cccagccaga cgcggttttc
     2701 gcatccggca atctctttag cctgcgcttt taactcctct ggcaaagccg gaagctgttt
     2761 ccccagcatg atcaactggc gatatttatc ttcccattgc gtcaacggtg cgaaggtatt
     2821 gcgtaacgtt tctgcggtta cggttgtgcc gaacggatgt ccggcgaatt gcgggtttgt
     2881 cattaatcca ccaataattc cagcgcgcgg tcaacggcat tcaccagcgc atccacatca
     2941 ctctttgtat tatatggcgc aaatgaggcg cgcagtgtgc cggttacgcc taattctgcc
     3001 agtagcggct gagcgcaatg ctgcccggcc cgcagggcaa taccgtactc cgccagcagc
     3061 gtcaccatat cgctgtgatg aacgccagca aaatcaaagg ccagcaggct ggaatcctgg
     3121 cagcgaaatg agcggaagcc aggacgtttt gccagcgcat cttccgccag cgttgctaag
     3181 ctacggctcc agctttcggc ctggttgata tcgtaatctg ccagccattc cagcgccgcg
     3241 cttaatccta tgacaccagc gacatttggc gttccagctt ccagtttcca cggcgcagat
     3301 tgaggcgtga agccgtcaaa actcacttcg tgaaccattt tgccgccgcc cagccagggc
     3361 gacatcgctt ccagcagttc tgatttgcca tacagcacgc cgatacctgt cgggccatac
     3421 agtttgtgac ctgaaaaagc atagaaatca atatccagtt gctgaacatc cgcggggaaa
     3481 tgcaccgccc cctgagcacc atcaaccatc accaccatcc cggctgaatg agcaaaggta
     3541 atcgctcgcg ccagatccgg gcaaccgcca gtgacgttcg acatttgtcc cagagcaaga
     3601 attcgactac gaggtgtgat cagttctggc aacaaatcga catccggcag tcgctgcgca
     3661 ttaagcggca atttcaccac tttggctcca gtttgttggg cgaccatcag ccaggggacg
     3721 aggttggcgt ggtgttctgc cacgctgaca ataatctcat cgcccggttg cagatgcgga
     3781 cgcgcatagc attgcgccac catgttgatg gattcagtgg tgccgcgcgt ccagacgata
     3841 gttttgtcat ctggtgcatt cagtaattgc gccactttct cccgtgcagc ttcataacgc
     3901 gcggtcaggc gttgggcttc ggcaaactgg ctgcgatgga cgtttccggc gctcaggctg
     3961 taaaactgtc gggtggcttc aaccacggct tcaggtttaa gcgtggtcgc ggcgctgtcg
     4021 agatagacgc ccgcatcctg tagcgcggga aactgggcgc gaaactgcgc gggattaaaa
     4081 acgttcatgg tactcctcgg cttgatgcac cgatcgtgac gcaaaattca ctttgataca
     4141 aggagagtgc taaccatcag aattatctgg atatcctggc gaagccgcgt cagtaacata
     4201 tgctgaatag tgttaggcgt taattttagc ctgttaatca atactattaa gatttattaa
     4261 tcgctttaag gagaagatga tgaaaaagac tgccgcaatt atttctgcct gtatgctgac
     4321 ttttgccctg agcgcctgtt ccggttcgaa ctatgtgatg cacaccaatg acggacgtac
     4381 catcgtctct gacggcaaac cacagactga taacgatacc ggtatgattt cgtataaaga
     4441 cgctaatggc aacaaacagc agatcaaccg taccgacgtg aaagagatgg tcgaactgga
     4501 tcagtaattc gcgatcgcaa ggtaaaaaaa agcaccgcaa ttaggcggtg ctacattaat
     4561 cactatggac agacagggta aatgtacagg aagtgaaaaa aggtagcttt gctaccatgg
     4621 tctgaatcgc agaccaattg caaacacaac aacacaacat cacaaccgta agccaaaagt
     4681 tcaccagaac acgcattccg ataaaacttt tcgttccggc tcaggaagtg ccgccactat
     4741 aggtatttgc tggtagaagc tcaacggaca atttataatg gctcagatta aaaa
//
LOCUS       DAZFLD010000270         4766 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.272, whole genome shotgun sequence.
ACCESSION   DAZFLD010000270 DAZFLD010000000
VERSION     DAZFLD010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4766)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4766)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4766
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.272"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..593)
                     /locus_tag="ACHKBL_004434"
     CDS             complement(<1..593)
                     /locus_tag="ACHKBL_004434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFO5821198.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDTPTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVAATDGPMPQTREHILLGRQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQ
                     YDFPGDDTPIVRGSALKALEGDAEWEAKILELAGFLDS"
     gene            complement(708..783)
                     /locus_tag="ACHKBL_004435"
     tRNA            complement(708..783)
                     /locus_tag="ACHKBL_004435"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(748..750),aa:Thr,seq:ggt)
     gene            complement(790..864)
                     /locus_tag="ACHKBL_004436"
     tRNA            complement(790..864)
                     /locus_tag="ACHKBL_004436"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(829..831),aa:Gly,seq:tcc)
     gene            complement(981..1065)
                     /locus_tag="ACHKBL_004437"
     tRNA            complement(981..1065)
                     /locus_tag="ACHKBL_004437"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1029..1031),aa:Tyr,seq:gta)
     gene            complement(1074..1149)
                     /locus_tag="ACHKBL_004438"
     tRNA            complement(1074..1149)
                     /locus_tag="ACHKBL_004438"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1114..1116),aa:Thr,seq:tgt)
     gene            1511..2461
                     /gene="coaA"
                     /locus_tag="ACHKBL_004439"
     CDS             1511..2461
                     /gene="coaA"
                     /locus_tag="ACHKBL_004439"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HFO5821199.1"
                     /translation="MSIKEQTLMTPYLQFDRNQWAALRDSVPMTLSEDEIARLKGINE
                     DLSLEEVAEIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGQRIPYIISIAGSVAVGKS
                     TTARVLQALLSRWPEHRRVELITTDGFLHPNQVLKERGLMKKKGFPESYDMHRLVKFV
                     SDLKSGVPNVTAPVYSHLIYDVIPDGDKTVVQPDILILEGLNVLQSGMDYPHDPHHVF
                     VSDFVDFSIYVDAPEDLLQTWYINRFLKFREGAFTDPDSYFHNYAKLTKEEAIKTAMT
                     LWKEINWLNLKQNILPTRERASLILTKSANHAVEEVRLRK"
     gene            complement(2490..3455)
                     /gene="birA"
                     /locus_tag="ACHKBL_004440"
     CDS             complement(2490..3455)
                     /gene="birA"
                     /locus_tag="ACHKBL_004440"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HFO5821200.1"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIQLLNAEKILSQLDDGSVAVLPVIDSTNQYLLDRIGELK
                     SGDACVAEYQHAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEV
                     LRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESV
                     VNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLTPYLSRWEKLDNFINRPV
                     KLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     gene            complement(3452..4480)
                     /gene="murB"
                     /locus_tag="ACHKBL_004441"
     CDS             complement(3452..4480)
                     /gene="murB"
                     /locus_tag="ACHKBL_004441"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HFO5821201.1"
                     /translation="MNHSLKPWNTFGIDHNAQHIVCAEDEQQLLNAWQHATAKGQSVL
                     ILGEGSNVLFLEDYRGTVIINRIKGIEIHDEPDAWYLHVGAGENWHRLVKYTLQEGMP
                     GLENLALIPGCIGSSPIQNIGAYGVELQRVCAYVDCVELATGKQVRLTAKECRFGYRD
                     SIFKHEYQDRFAIVAVGLRLPKEWQPVLTYGDLTRLDPSTVTPQQVFDAVCHMRTTKL
                     PDPKVNGNAGSFFKNPVVSAETANALLAQFPTAPHYPQVDGSVKLAAGWLIDQCQLKG
                     TQIGGAAVHRQQALVLINEHDAKSEDVVQLAHHVRQKVGEKFNVWLEPEVRFIGASGE
                     VSAVETIS"
     gene            complement(4617..4732)
                     /gene="rrf"
                     /locus_tag="ACHKBL_004442"
     rRNA            complement(4617..4732)
                     /gene="rrf"
                     /locus_tag="ACHKBL_004442"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         1209 a         1207 c         1089 g         1261 t
ORIGIN      
        1 gaatccagga agccagccag ttccaggatt ttcgcttccc actctgcgtc gccttccagc
       61 gctttcagag cagaaccacg aacgatcgga gtgtcgtcgc ccgggaagtc gtactgagac
      121 agaagttcac gaacttccat ttcaaccagt tccagcagct cttcgtcatc aaccatgtcg
      181 catttgttca ggaacacgat gatgtacgga acgcctacct gacgccccag caggatgtgc
      241 tcacgagtct gcggcatcgg gccgtcagtc gcagcaacta ccaggatcgc gccgtccatc
      301 tgagcagcac cggtgatcat gtttttaaca tagtcggcgt gccccgggca gtctacgtgc
      361 gcgtagtgac gggtcggggt gtcgtattca acgtgagaag tgttgatggt gataccacga
      421 gctttttctt ccggcgcgtt atcgatctgg tcgaatgcgc gagcagcacc gccgtaggtt
      481 ttagccagta cggtagtgat tgcagcggtc agcgttgttt taccatggtc aacgtggccg
      541 atagtaccga cgttaacgtg cggttttgta cgttcaaact tttctttaga catcgattgt
      601 ccctctaaga cacggataaa tcggtgatat caccacatca accaggcaac atgcccgact
      661 tgttgaatgc aataaacaga aggaaaaaac agggaggaga aaagaagtgg tgctgatagg
      721 cagattcgaa ctgccgacct cacccttacc aagggtgcgc tctaccaact gagctatatc
      781 agcacatctt ggagcgggca gcgggaatcg aacccgcatc atcagcttgg aaggctgagg
      841 taatagccat tatacgatgc ccgcatcctg gaactcggct acctgatttt cattctgcac
      901 tgaatatcga gagaagctct ctttattcga gccggtaagc gaacttatcg tctcgggcta
      961 cgccatcgcg tggccgaaat tggtggtggg ggaaggattc gaaccttcga agtctgtgac
     1021 ggcagattta cagtctgctc cctttggccg ctcgggaacc ccaccggact tgatggtgcc
     1081 gactaccgga atcgaactgg tgacctactg attacaagtc agttgctcta cctactgagc
     1141 taagtcggca tcaagtagcg cgcattctat ggagacatgc gagttcatgc aactaaaaaa
     1201 ttgcataatt tgttttattg gtcacatttt atgcgacacg atgaagaaac agccgaacgg
     1261 agggcagatt agcacacttt ttcaacatca ttgtgctcaa caatgtgctc ctgctaaacc
     1321 ataattcttt ttatcagatg gaatatctgt cacattgctt ttcaacgata gcttcctggg
     1381 agagattttt tcttattatt cctccccatc tggtgttacc ctcctgccca ttaacccatt
     1441 caacagaact gtgacgcgcc atggcaaata tcgctttgcc gatagagcta tgaccgcctg
     1501 aaacatgctt atgagtataa aagagcaaac gttaatgacg ccttacctac agtttgaccg
     1561 caaccagtgg gcagctctgc gtgattccgt acctatgacg ttatcggaag atgagatcgc
     1621 ccgtctcaaa ggtattaatg aagatctctc gttagaagaa gttgccgaga tctatttacc
     1681 tctgtcacgt ttgctgaact tctatataag ctcgaatctg cgccgtcagg cagttctgga
     1741 acagtttctt ggtaccaacg ggcaacgcat tccttacatt atcagtattg ctggtagtgt
     1801 cgcggtgggg aaaagtacaa ccgcccgtgt attgcaggcg ctattaagcc gttggccgga
     1861 acatcgtcgt gttgaactga tcactacaga tggcttcctt caccctaatc aggttctgaa
     1921 agaacgtggt ctgatgaaga agaaaggctt cccggaatcg tatgatatgc atcgcctggt
     1981 gaagtttgtt tccgatctca aatccggcgt gccaaacgtt acagcccctg tttactcgca
     2041 tcttatttat gatgtgatcc cggatggaga taaaactgtt gttcagcctg atattttaat
     2101 tcttgaaggg ttaaatgtct tacagagcgg gatggattat ccacacgatc cacatcatgt
     2161 atttgtttct gattttgtcg atttttcgat atatgttgat gcaccggaag acttacttca
     2221 gacgtggtat atcaaccgtt ttctgaaatt ccgcgaaggg gcttttacag acccggattc
     2281 ctattttcat aactacgcga aattaactaa agaagaagct attaagactg ccatgacatt
     2341 gtggaaagag atcaactggc tgaacttaaa gcaaaatatt ctacctactc gcgagcgtgc
     2401 cagtttaatc ctgacgaaaa gtgctaatca tgcagtagaa gaggtcagac tacgcaaata
     2461 atttaaaggg gagttaatgc tcccctttct tatttttctg cactacgcag ggatatttca
     2521 ccgcccatcc agggttttat tattccatcc tgctcaagta ataaagcacc ctgtttgtct
     2581 attccgcgtg aaatgccaaa tatttcttta tcaccaatga taagtttcac tgggcgatta
     2641 ataaaattat ccaacttttc ccagcgcgaa agataaggtg tcaatccttc ttgctcgaag
     2701 agttccagcg ccgcgcgtaa ttcacgtatc agcatggccg caagagtatt acgatcgaga
     2761 ttgatcccgg cttcctgcag cgtgatccac ccttgattaa cgacactctc ttcaacacga
     2821 cgcattgcca tgttgatccc tgcaccaatg actatttgcg ccgcatcgcc ggttttgcca
     2881 gtcagctcca caagaatgcc tgccagcttg cgatcctgca gatagaggtc attaggccat
     2941 ttaacacgaa ctttatctgc acccagcttg cgtaatactt ccgccatcac gataccgata
     3001 accagactta aaccaatcgc cgccgccggg ccctgttcca gacgccagaa catcgacaaa
     3061 tataagttcg cgccaaaagg cgaaaaccat ttccgaccac gacgaccacg gccagcatgc
     3121 tggtattctg caacacaggc atcgccagat ttaagctctc cgatgcggtc caggagatac
     3181 tgattcgtgg agtcaataac tggcagcacg gctacactgc cgtcatccag ctgactcaat
     3241 atcttttcgg cattaagtaa ctggattggc tcgggcaagc tgtatccttt acccggaaca
     3301 gtaaatacat caacgcccca gtcacgcagt gtctgaatgt gtttattaat agccgcccgg
     3361 ctcattccca gcgtttcacc caactgctcg ccagagtgaa attcgccatt cgctaacagg
     3421 gcgatcaatt tcagtggcac ggtgttatcc ttcatgaaat tgtctccact gcgctcactt
     3481 cacctgatgc accaataaag cggacttcag gctcaagcca gacattaaat ttttcaccca
     3541 ctttttgtct tacatgatgc gccagctgta caacatcttc gctttttgca tcgtgctcat
     3601 ttatgagaac cagcgcctgt tgacggtgta ccgcagcccc accaatttgc gtccctttaa
     3661 gctggcactg atcaataagc caacctgcag ccagttttac tgaaccatcc acctgggggt
     3721 aatgtggggc tgttggaaat tgtgccaaca acgcgttagc agtttcggca gaaacaacag
     3781 gatttttgaa gaaactaccc gcattaccgt tcacttttgg atcagggagt ttggtggtgc
     3841 gcatatgaca caccgcatca aatacttgct gtggcgttac cgttgaagga tccaggcgag
     3901 ttaagtcacc atacgtcagt acaggttgcc actcttttgg cagacgcaga cctacggcta
     3961 caatggcgaa gcgatcctgg tattcatgtt taaaaatact gtctcgatag ccaaaacggc
     4021 actctttggc agttaagcgt acttgcttgc ctgtcgccag ttcaacacaa tcaacataag
     4081 cgcaaactcg ctgtaattct acgccataag caccaatatt ctggataggt gatgagccga
     4141 tacaaccagg aattaatgcc agattttcca gaccaggcat accttcctgc aaagtgtatt
     4201 ttaccaggcg atgccagttt tctccggctc ctacatgtaa ataccacgca tcaggttcat
     4261 catgaatttc gatacctttg atccggttga tgatcaccgt accgcgatag tcttccagaa
     4321 aaagtacatt acttccttca cccagaataa gaacggattg tccttttgcg gttgcatgct
     4381 gccaggcatt gagtagttgt tgttcgtctt cggcacatac aatgtgctga gcattatgat
     4441 caatgccaaa tgtgttccag ggttttaagg agtggttcat agctgctttc ctgatgcaaa
     4501 aacgaggcta gtttaccgta tctgcggggg gatggcttgt agatatgatg acaggaagag
     4561 tttgtagaaa cgcaaaaagg cccagtcttc cgactgggcc ttctgcttaa tttgatgcct
     4621 ggcagttccc tactctcgca tggggagacc ccacactacc atcggcgcta cggcgtttca
     4681 cttctgagtt cggcatgggg tcaggtggga ccaccgcgct aaggccgcca ggcaaattct
     4741 gtttcatcag accgcttctg cgttct
//
LOCUS       DAZFLD010000271         4714 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.273, whole genome shotgun sequence.
ACCESSION   DAZFLD010000271 DAZFLD010000000
VERSION     DAZFLD010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4714
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.273"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..191
                     /locus_tag="ACHKBL_004443"
     CDS             <1..191
                     /locus_tag="ACHKBL_004443"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5821202.1"
                     /translation="CEAYHIEIMELLAKLMNNSSKTSTVQIKRIKPSIIYRLLLIGLG
                     TPMVIYGLVRPLTIETRD"
     gene            complement(410..1948)
                     /gene="aldB"
                     /locus_tag="ACHKBL_004444"
     CDS             complement(410..1948)
                     /gene="aldB"
                     /locus_tag="ACHKBL_004444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase AldB"
                     /protein_id="HFO5821203.1"
                     /translation="MTNNPPSAQIKPGEYGFPLKLKARYDNFIGGEWVAPADGEYYQN
                     LTPVTGQLLCEVASSGKRDIDLALDAAHKVKDKWAHTSVQDRAAILFKIADRMEQNLE
                     LLATAETWDNGKPIRETSAADVPLAIDHFRYFASCIRAQEGGISEVDSETVAYHFHEP
                     LGVVGQIIPWNFPLLMASWKMAPALAAGNCVVLKPARLTPLSVLLLMEIVGDLLPPGV
                     VNVVNGAGGEIGEYLATSKRIAKVAFTGSTEVGQQIMQYATQNIIPVTLELGGKSPNI
                     FFADVMDEEDAFFDKALEGFALFAFNQGEVCTCPSRALVQESIYERFMERAIRRVESI
                     RSGNPLDSVTQMGAQVSHGQLETILNYIDIGKKEGADVLTGGRRKLLEGELKDGYYLE
                     PTILFGQNNMRVFQEEIFGPVLAVTTFKTMEEALELANDTQYDLGAGVWSRNGNLAYK
                     MGRGIQAGRVWTNCYHAYPAHAAFGGYKQSGIGRETHKMMLEHYQQTKCLLVSYSDKP
                     LGLF"
     gene            complement(2056..3351)
                     /locus_tag="ACHKBL_004445"
     CDS             complement(2056..3351)
                     /locus_tag="ACHKBL_004445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HFO5821204.1"
                     /translation="MAIKSPPGLIPLSHLSGEELLAHLRFNRVTDEKGRYLPFDELQY
                     RIKKGENVDVAWTLTRLARNAAIQRINYCNEAGEQAGFNITPVIAEACELVDKHATAL
                     ALKDQTERLRGAGAELSQLRLEEPITSSQLEGANTTTLVARKMLETGRSPRTEDEHMI
                     AGNARLMAEIPHLLAEPLTPALIRQLHAIGMGGINDAKYRPGEFRETDDVVIADYDGN
                     IVHQPPAAALLPERLEKVCQWLNSHEGYIHPLVRACILHFMLAHEHPFRDGNGRTSRA
                     LFYWYMLKSGYDVFKYISISRLLHAAPVKYAASYQYTESDGMDLTYFLEYQAGVIKRA
                     LQNWQQHIDEITQRSAKLDSVLFSSGVLKRLNPRQVTLLNVMLANPGKEYTVAEISAS
                     LGVSDNTARADLRTIVKEGFAQEKRINNQQAVYVAHYPL"
     gene            complement(3481..4632)
                     /gene="yiaY"
                     /locus_tag="ACHKBL_004446"
     CDS             complement(3481..4632)
                     /gene="yiaY"
                     /locus_tag="ACHKBL_004446"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine dehydrogenase"
                     /protein_id="HFO5821205.1"
                     /translation="MAASTFFIPSVNVIGADSLNDAMNMMADYGFTRTLIVTDNMLTK
                     LGMAGDVQKALEERNIFSVIYDGTQPNPTTENVAEGLKLLKENNCDSVISLGGGSPHD
                     CAKGIALVAANGGDIRDYEGVDRSAKPQLPMIAINTTAGTASEMTRFCIITDEARHIK
                     MAIVDKHVTPLLSVNDSSLMVGMPKSLTAATGMDALTHAIEAYVSIAATPITDACALK
                     AVTMIAENLPLAVEDGSNAKAREAMAYAQFLAGMAFNNASLGYVHAMAHQLGGFYNLP
                     HGVCNAVLLPHVQVFNSKVAAARLRDCAAAMGVNVTGKNDAEGAEACINAIRELAKKV
                     NIPAGLRDLNVKEEDIPVLATNALKDACGFTNPIQATHEEIMAIYRAAM"
BASE COUNT         1141 a         1249 c         1139 g         1185 t
ORIGIN      
        1 agtgcgaggc ttatcatatt gaaataatgg aactattggc caaactaatg aataactcca
       61 gtaaaacatc cacagtacag attaagcgta ttaaaccttc aattatctac cgtttattgc
      121 tgattggcct cggaacgcca atggtgattt atggcctggt tcgcccgctc accatcgaaa
      181 cgcgagatta aactcctggc aaaacacgcc ccagaaacgc acttctgttg acgtagtctg
      241 ggttattgcg cacgtaggtt taaaacgtaa attgtaacaa cgtgcgcttt gtttatgccg
      301 gatgcggcgt gtacgcctta tccggcctac ggtcaggtcc ccgtaggcat gataagacgc
      361 gtaagcgtcg catcaggcaa tgaataccca atgcgaccag cttcttatat cagaacagcc
      421 ccaacggttt atccgagtag ctcaccagca ggcacttggt ttgctggtaa tgctcaagca
      481 tcatcttgtg ggtttcgcgt ccgatgcccg actgtttgta gccaccaaac gccgcatgtg
      541 ccgggtaagc gtgataacaa ttggtccaca cgcgccctgc ctgtatgccg cgccccatct
      601 tataggcaag attaccgttg cggctccaga cgcccgcgcc caggtcatat tgcgtatcgt
      661 tcgccagctc cagcgcttct tccatcgttt tgaaggtggt caccgccagc accgggccaa
      721 aaatctcctc ctggaacacg cgcatattgt tctgaccaaa caaaatcgtc ggttcgaggt
      781 agtagccgtc tttcagttca ccttccagca gcttgcgccg cccgccagtg agcacgtctg
      841 cgccctcttt tttaccgata tcaatgtagt tgaggatggt ttccagttgc ccgtgagaaa
      901 cctgcgcccc catttgcgtc acgctgtcga gcgggttacc gctgcgaatg ctttcgacac
      961 ggcggatggc gcgttccata aagcgttcgt agatagactc ctgcactaaa gcacggcttg
     1021 gacaggtgca aacttcgccc tggttaaagg caaacagtgc aaagccttcc agcgctttat
     1081 cgaaaaaggc atcttcttca tccattacgt cagcaaagaa gatatttggc gatttgccac
     1141 ccagctccag cgtcaccgga ataatgtttt gcgtggcgta ttgcataatt tgttggccca
     1201 cttccgttga gccggtaaac gccactttgg cgatgcgttt cgaggtcgcc agatattcgc
     1261 caatttcccc acctgcgccg ttgaccacgt tcaccacgcc cggcggcagt aaatcaccga
     1321 cgatttccat tagcagcagt acagaaagcg gggtaagacg tgcgggtttc agcaccacac
     1381 agttgcccgc cgccagcgcg ggagccattt tccagctcgc catcagcagc gggaagttcc
     1441 acggaataat ctgccccacc acgcctaacg gctcatggaa atgataggcc acggtttcgc
     1501 tatcaacttc actgatccca ccttcctgcg cccgaataca cgaggcaaaa tagcgaaaat
     1561 gatcaatcgc cagcggcaca tcagcagcac tggtttcgcg aatgggtttg ccgttatccc
     1621 aggtttcagc ggtcgctaac agttcaaggt tttgttccat tcgatcggca atcttaaaca
     1681 gaatcgccgc acgatcctgc accgaggtgt gcgcccattt atctttcact ttgtgcgcag
     1741 catccagcgc cagatcgatg tctcgtttgc ccgaagacgc cacttcgcac agcagctgcc
     1801 cggtcaccgg cgtcaggttc tggtaatact cgccatcggc aggcgctacc cattcgccac
     1861 cgataaagtt gtcgtagcgg gcttttaatt tgagggggaa accatactcg ccgggcttaa
     1921 tctgtgctga agggggatta ttggtcatga tcgtctcctt gcggtgtgag gtataacaag
     1981 ggtagacgtg actggcgaaa tcttcgccag tcggtaacag ctttacgacg gctatcacga
     2041 atttacgggc aaaagctaca gcgggtagtg tgcgacgtac acggcttgct ggttattaat
     2101 tcttttctct tgcgcgaatc cttctttaac aattgtccgt aaatcagctc gagccgtatt
     2161 atcagagacg cccaagctcg cgctaatttc agcaactgtg tactctttcc ccggattggc
     2221 gagcatcaca ttcaggagcg ttacctggcg cggattcagg cgtttaagca caccagagct
     2281 aaacagcaca ctatcgagtt ttgcgcttcg ttgtgtaatt tcatcgatat gctgctgcca
     2341 gttttgcaga gcacgtttaa tgacacccgc ctgatattca aggaaataag tcagatccat
     2401 accatctgat tcagtgtact ggtagctggc ggcatatttg accggtgcag catgaagcaa
     2461 acggctgatg gaaatatatt taaagacatc gtagcccgat ttcagcatat accagtaaaa
     2521 cagcgcccga ctggtgcgcc cgttgccatc gcgaaacgga tgttcatggg ccagcataaa
     2581 atgcagaatg caggcacgga ccaacggatg aatataccct tcatggctgt ttaaccactg
     2641 gcaaactttt tccagccgtt ccggtaacaa tgccgcagcg ggcggttgat gcactatatt
     2701 gccatcatag tcggcgatca caacatcatc tgtttcacga aattctcccg gacgatattt
     2761 cgcgtcattg attccaccca taccaatggc atgtaactgg cgaataagcg caggtgtcag
     2821 cggttctgcg agtaagtgcg gaatttccgc catcagccgg gcatttcccg caatcatatg
     2881 ctcgtcttca gtgcgtggcg accgccctgt ttccagcatt tttctcgcca ccagcgttgt
     2941 agtattcgca ccttccagtt gggaactggt gatcggctct tccaggcgta actgacttaa
     3001 ctcggcgccc gccccgcgta accgttcagt ctgatctttc agcgccagtg cagtggcatg
     3061 tttatcaaca agctcgcacg cttctgcaat caccggcgta atgttgaatc cggcctgttc
     3121 acccgcttca ttgcaataat taattcgttg gatcgccgcg ttgcgcgcca gacgggtaag
     3181 cgtccacgcc acatcgacgt tttctccttt tttgatgcgg tactgcagct catcaaaagg
     3241 cagataacgt cctttttcat cggtaacccg gttaaagcgc aggtgggcca ggagttcctc
     3301 acccgataag tgtgataaag gaataagtcc gggaggtgat ttgatagcca tgtgcttatc
     3361 tcaaaaaagc gcaatattga gatagtgtat agcaggaaag agaaactatc tcaaaaatcg
     3421 caaattttga gatagtttaa aaaatggctg acaattagac agccattgtg ggaatgatga
     3481 ttacatcgct gcgcgataaa tcgccataat ttcttcgtga gttgcctgaa tcgggttagt
     3541 aaacccacac gcatctttca gggcattggt tgccagcact ggaatatctt cttctttcac
     3601 gttcaggtca cgcaggcctg ccgggatatt cactttcttc gccagttcac ggatggcatt
     3661 gatgcacgct tctgcgcctt ccgcgtcatt tttgcctgtc acgttaacgc ccatggcagc
     3721 agcacagtca cgcagacgcg cagcagcgac tttgctgttg aacacctgaa cgtgtggcag
     3781 cagaacggca ttacatacac catgtggcag gttgtagaaa ccgcccagtt ggtgcgccat
     3841 cgcatgaaca taaccaagag atgcgttgtt gaatgccatg ccagcgagga actgggcata
     3901 agccattgct tcacgcgctt tagcattgct gccatcttca acggctaacg gcaggttttc
     3961 ggcaatcatg gtcacggctt tcagtgcgca agcgtcagtg atcggcgtgg cggcaataga
     4021 aacatacgct tcgatagcgt gcgttaaggc atccataccc gttgcggcgg tcagtgactt
     4081 cggcataccg accatcagag atgagtcatt gacagaaagc aacggagtta catgtttatc
     4141 aacaatcgcc attttgatat gacgcgcttc gtcagtgatg atgcagaaac gggtcatttc
     4201 agaggccgta cccgccgtgg tattgatggc gatcatcggc agctgcggtt ttgcagagcg
     4261 gtcaacgcct tcgtaatcgc gaatatcgcc gccattggct gccaccagcg caataccttt
     4321 tgcgcagtcg tgcggagaac caccgcctaa ggagatcacg ctatcgcaat tattttcttt
     4381 aagtaatttt aaaccttcgg cgacgttttc cgtggtgggg ttaggttggg tgccatcata
     4441 aataacgcta aaaatattgc gttcttccag tgctttttgc acatcacccg ccatacccaa
     4501 tttcgttaac atgttgtcgg tgacaattaa ggtacgggta aatccataat ctgccatcat
     4561 attcattgca tcattcaatg aatcagcacc aataacattc acggaaggaa taaaaaaggt
     4621 tgaagctgcc ataacacact cctgaaagtg taaaaaaggg cgggcagctt actgaggatt
     4681 ttcatttgaa aatatgatca acattaccgt ttat
//
LOCUS       DAZFLD010000272         4679 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.274, whole genome shotgun sequence.
ACCESSION   DAZFLD010000272 DAZFLD010000000
VERSION     DAZFLD010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4679
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.274"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..153)
                     /locus_tag="ACHKBL_004447"
     CDS             complement(<1..153)
                     /locus_tag="ACHKBL_004447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel protein MscS"
                     /protein_id="HFO5821206.1"
                     /translation="MIAELFTNNALNLVIIFGSCAALILMSFWFRRGNRKRKGFLFHA
                     VQFLIYT"
     gene            397..654
                     /gene="ynaJ"
                     /locus_tag="ACHKBL_004448"
     CDS             397..654
                     /gene="ynaJ"
                     /locus_tag="ACHKBL_004448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2534 family protein"
                     /protein_id="HFO5821207.1"
                     /translation="MIMAKLKSAKGKKFLFGLLAVFIIAASVVTRATIGGVIEQYNIP
                     LSEWTTSMYVIQSSMIFVYSLVFTVLLAIPLGIYFLGGEEQ"
     gene            complement(704..1654)
                     /gene="uspE"
                     /locus_tag="ACHKBL_004449"
     CDS             complement(704..1654)
                     /gene="uspE"
                     /locus_tag="ACHKBL_004449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspE"
                     /protein_id="HFO5821208.1"
                     /translation="MAMYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKAFLPIYDF
                     SYEMTTLLSPDERTAMRQGVISQRTAWIHEQAKYYLNAGVPIEIKVVWHNRPFEAIIQ
                     EVISGGHDLVLKMAHQHDRLEAVIFTPTDWHLLRKCPSPVWMVKDQPWPEGGKALVAV
                     NLASEEPYHNALNEKLVKETIELAEQVNHTEVHLVGAYPVTPINIAIELPEFDPSVYN
                     DAIRGQHLLAMKALRQKFGINENMTHVEKGLPEEVIPDLAEHLQAGIVVLGTVGRTGI
                     SAAFLGNTAEQVIDHLRCDLLVIKPDQYQTPVELDDEEDD"
     gene            complement(1806..2558)
                     /gene="fnr"
                     /locus_tag="ACHKBL_004450"
     CDS             complement(1806..2558)
                     /gene="fnr"
                     /locus_tag="ACHKBL_004450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HFO5821209.1"
                     /translation="MIPEKRIIRRIQSGGCAIHCQDCSISQLCIPFTLNEHELDQLDN
                     IIERKKPIQKGQTLFKAGDELKSLYAIRSGTIKSYTITEQGDEQITGFHLAGDLVGFD
                     AIGSGHHPSFAQALETSMVCEIPFETLDDLSGKMPNLRQQMMRLMSGEIKGDQDMILL
                     LSKKNAEERLAAFIYNLSRRFAQRGFSPREFRLTMTRGDIGNYLGLTVETISRLLGRF
                     QKSGMLAVKGKYITIENNDALAQLAGHTRNVA"
     gene            complement(2753..3268)
                     /gene="ogt"
                     /locus_tag="ACHKBL_004451"
     CDS             complement(2753..3268)
                     /gene="ogt"
                     /locus_tag="ACHKBL_004451"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HFO5821210.1"
                     /translation="MLRLLEEKIATPLGPLWVICDEQFRLRAVEWEEYSERMVQLLDI
                     HYRKEGYERISAANPGGLSDKLREYFAGNLSIIDTLPTATGGTPFQREVWKTLRTIPC
                     GQVMHYGQLAEQLGRPGAARAVGAANGSNPISIVVPCHRVIGRNGTMTGYAGGVQRKE
                     WLLRHEGYLLL"
     gene            complement(3279..3422)
                     /locus_tag="ACHKBL_004452"
                     /pseudo
     CDS             complement(3279..3422)
                     /locus_tag="ACHKBL_004452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015558 - secondary active
                     p-aminobenzoyl-glutamate transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902604 - p-aminobenzoyl-glutamate
                     transmembrane transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
     gene            3422..3638
                     /locus_tag="ACHKBL_004453"
                     /pseudo
     CDS             3422..3638
                     /locus_tag="ACHKBL_004453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000885449.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(3812..4627)
                     /locus_tag="ACHKBL_004454"
     CDS             complement(3812..4627)
                     /locus_tag="ACHKBL_004454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707689.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO5821211.1"
                     /translation="MTITLQPKASPGHHIIGLDSEHLNGGTVPWHKHLYAQLLYPAEG
                     VVRVWAGESVWLVHASSALWLPPQMPHKFVATGNVLLKTVLVSEAESETLGKVCFMTG
                     ISPLLRELLIAINQLPPSQSTTDKQQLRFSALETLILQEIKMGVKMSLELPWPNDERL
                     QQLCENLLNNQGYLPTLDNLADKINVSSRTLMRLFVKETGLTFRHWVQQMHVISAVTL
                     LDDGYSLTKIAHRLGYASAESFGNMFKRRTGYSPGKFTRRLTMHDYAITRQMI"
BASE COUNT         1225 a         1103 c         1092 g         1259 t
ORIGIN      
        1 ggtgtagatt aaaaattgca ccgcatggaa taaaaatcct tttcttttac gatttccgcg
       61 gcgaaaccaa aagctcatca gaatcaatgc tgcgcagcta ccgaaaataa tgaccagatt
      121 aagcgcatta tttgtaaaca gttcagcgat cattgtttta tcaggctcct ccagataatt
      181 gtcgtcatgc cggaaacccc tggcggggct attttaccgc gacaattcat tcagatcatc
      241 aatagtcagg gaaggaagta gcaatattag ctaactaatc aatgcgttga ttgtaaatcc
      301 agctaagagg tgaggttttc agagcagaca acggtgaaat gtcatggtat tgttacgttt
      361 aggtaacaag aaatttgtct gcacaaggat tacatcatga ttatggcgaa actgaagtca
      421 gcgaaaggga agaaatttct ctttggtttg ttggcggttt tcattattgc ggcgtcggtt
      481 gtgactcgcg cgaccatcgg cggcgtgata gaacagtaca atattccgct gtctgagtgg
      541 acgacatcaa tgtatgtgat tcagtcatcg atgatttttg tttatagcct ggtctttact
      601 gtgttgctgg caatcccgtt gggaatttat ttccttggcg gcgaagagca gtaagtaaaa
      661 aataggcccg ataactcggg ccttgtcagt tattgaagag tcgttaatcg tcttcttcgt
      721 catccagttc aacgggtgtc tgatactggt caggtttaat gaccagcagg tcgcagcgaa
      781 gatgatcaat cacctgttcc gccgtgttac caaggaatgc tgctgaaata ccggtgcgtc
      841 cgaccgtgcc cagaaccaca atccccgcct gtaagtgctc cgccaaatca ggaatcacct
      901 cttctggcag acctttttct acgtgcgtca tgttttcatt aatgccgaat ttctgccgca
      961 gggctttcat tgccagcaaa tgttgcccac gaatggcatc gttataaacg ctcggatcaa
     1021 attccggcag ttcaatcgcg atattaattg gcgttaccgg ataagcgcca accagatgaa
     1081 cttcggtatg gttgacttgt tccgccagtt cgatcgtctc tttgaccagt ttttcattga
     1141 gcgcattatg atacggctct tcactggcga gattcaccgc caccagcgcc ttaccccctt
     1201 ccggccacgg ctggtctttc accatccaca cggggcttgg gcatttgcgt aacagatgcc
     1261 agtccgttgg cgtaaaaatc accgcttcca gacggtcatg ttggtgcgcc atttttagca
     1321 ccaaatcgtg cccgccgctg atcacttcct gaatgatggc ttcgaaagga cggttatgcc
     1381 agaccacttt aatttcaatg ggaacgccag cattgagata atattttgcc tgctcgtgga
     1441 tccaggctgt acgctggctg atgacgccct gacgcatagc ggtacgttcg tccggggaga
     1501 gcagggtggt catttcgtat gagaagtcat agatcggcaa aaaggcttta attttgccac
     1561 caatccgttg atgtaaataa acagctcgcc gcaatgctgg ttggtcgtcc tggttaggat
     1621 cgataacaac gagcatgttc tgatacatag ccatacaggg tctccttaca acaactgtca
     1681 acgcagtttg taattaaaag attaacccat atctggtgaa tgaaacagtg atgaaccttc
     1741 tgccagatca ataaatcaga aaaatttaat gatatgacag aaggatagtg agttatgcgg
     1801 aaaaatcagg caacgttacg cgtatgacca gcaagctggg ccagcgcatc gttattttcg
     1861 atggtgatgt atttaccttt gactgccagc ataccgcttt tctggaagcg acccagcaga
     1921 cggctgatgg tttctaccgt caggcccaga tagttaccaa tatcgccacg agtcatcgtc
     1981 aggcggaatt cacgagggga gaagccgcgt tgggcaaaac gacgggacag gttgtagatg
     2041 aatgcagcca gacgttcctc ggcatttttc ttcgacaaca gcaggatcat gtcctgatcg
     2101 cctttgattt caccgctcat cagacgcatc atctgctgac gcagattcgg cattttaccg
     2161 gacaaatcgt ccagcgtttc gaacgggatt tcacatacca tcgaggtttc cagcgcctgc
     2221 gcgaagctcg ggtgatggcc gctgccgatg gcgtcaaatc ccaccaggtc gcctgctaaa
     2281 tggaaaccag tgatttgctc gtcgccttgc tcagtgatgg tataactttt aatcgtaccg
     2341 gagcggatgg cataaagcga tttaagttca tcaccagcct taaacagcgt ctggcctttc
     2401 tgaataggct tcttccgctc aatgatatta tcaagctgat caagctcatg ttcgttgagt
     2461 gtgaacggga tgcaaagctg gctgatgctg caatcctggc aatggatagc acaaccgcca
     2521 gactgaatgc gccgtataat tcgcttttcc gggatcatag gtctgctcaa gccgtaattg
     2581 atatttgtca attttaacat ctttttaggg agcaagtaag tctaagcaaa ccttaacagc
     2641 agagaattcc gatattagat gtaaatatat gtctatctat ttgaaaaccc ttaagttgtt
     2701 aagggtaact ttacataaaa gtgtgaacaa gctggcacaa attgtttaat gtttacagca
     2761 aaagataacc ttcgtggcgc aataaccact cttttcgctg aactccgcct gcatatccgg
     2821 tcatggtgcc gtttcggcca ataacccgat ggcaaggtac aacaatgctg atgggattcg
     2881 atccgttagc cgcaccaacg gcacgcgccg cgccagggcg acccaattgt tcggccagtt
     2941 gaccgtaatg cattacctgc ccgcagggga tagtgcgtag tgttttccag acttcgcgct
     3001 gaaatggcgt cccccccgtg gcagtgggaa gcgtatcaat aatgctaaga ttaccggcaa
     3061 aatattcacg aagcttgtcg cttaaaccac ctgggttggc ggcagaaatg cgctcatagc
     3121 cttctttgcg ataatggatg tccagcagct gcaccatgcg ttcgctgtac tcttcccatt
     3181 caaccgcccg caggcgaaat tgctcatcgc aaatcaccca tagtggaccc agtggcgtgg
     3241 caattttttc ttcaagtaat ctcagcatcc gttctctctt aagacaaacg tgggtaaatc
     3301 cccggaccta tcggcagacc aacaagatac cacgccagca acatcagcag ccataccacc
     3361 aaaaagataa gcggataggg caagactaac gaatagtaag tacccagttt cgcgtctggt
     3421 ttgtcgcagt ctgatagcat aaagcgattt tttgattttt ctgcctgaag aaatgagggc
     3481 aagccatcac atggatcgag ggtgggataa ttccagtcag cgaaatttta ccgtaggcta
     3541 tctgttattt gattctgtgg cgtgatagcc gccagacacc actgatctcg tcactcattc
     3601 cccaatcccg gcgagaatgt ggttatttac agagctaaaa aatgaataaa aaaatagagc
     3661 ctgtcgacat ccgccagact ctacagtaca cacagcagtg catcctcgtc ttaaatccgg
     3721 cctggggtcc tggtgaccgg tgacagtgtt ataacggttt tacccatccg ttgccaattt
     3781 tcttcagtga cagatttcac gaaaatataa gttaaatcat ttgccttgtg attgcataat
     3841 catgcatcgt taatcgacga gtaaatttac cgggcgagta gcccgttcga cgcttaaaca
     3901 tattgccgaa cgattcagct gaggcatagc ccagacgatg ggcaattttg gtcaacgaat
     3961 atccatcatc aagaagcgtc actgcagaaa taacgtgcat ttgctgaacc cagtggcgaa
     4021 atgttaatcc cgtctcttta acaaataagc gcatgagtgt acggctactg acgttaattt
     4081 tatccgctag attatccagt gttggcagat acccttggtt gtttagcaaa ttctcacaaa
     4141 gctgctgtag ccgctcatca tttggccaag ggagttcaag tgacattttc acccccattt
     4201 ttatttcctg aagtatcagt gtctcgaggg ctgaaaaacg taactgttgt ttatctgtgg
     4261 tactttgcga cggtggaagt tggttaattg caattagtaa ttctcttaac aggggtgaaa
     4321 tacccgtcat gaagcaaact tttcccagcg tttcactttc cgcttctgaa accagtacgg
     4381 tttttaaaag tacgttgcct gtcgcaacaa acttatgcgg catttgtggg ggcagccaaa
     4441 gcgcgctgct ggcatgcact agccagacgc tttctccagc ccatacacga acgactcctt
     4501 cagccgggta aagcaactgg gcatagagat gtttatgcca gggaactgtg cctccattca
     4561 gatgttccga atccagtccg ataatgtgat gccctggtga tgctttgggt tgcagtgtga
     4621 ttgtcattta cagtaggtat tttgtcagaa atcatcaaat aagccatgtt aaattttaa
//
LOCUS       DAZFLD010000273         4675 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.275, whole genome shotgun sequence.
ACCESSION   DAZFLD010000273 DAZFLD010000000
VERSION     DAZFLD010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4675
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.275"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            134..1366
                     /gene="dgcM"
                     /locus_tag="ACHKBL_004455"
     CDS             134..1366
                     /gene="dgcM"
                     /locus_tag="ACHKBL_004455"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcM"
                     /protein_id="HFO5821212.1"
                     /translation="MITHNFNTLDLLTSPVWIVSPFEEQLIYANSAARLLMQDLTFSQ
                     LRTGPYSVSSQKELPKYLSDLQNQHDIIEILTVQRNEEETALSCRLVLRKLTEAEPVI
                     IFEGIEAPATLGLKASRSANYQRKKQGFYARFFLTNSAPMLLIDPSRDGQIVDANLAA
                     LNFYGYNHETMCQKHTWEINMLGRRVMPIMHEISHLPGGHKPLNFVHKLADGSTRHVQ
                     TYAGPIEIYGDKLMLCIVHDITEQKRLEEQLEHAAHHDAMTGLLNRRQFYHITEPGQM
                     QHLAIAQDYSLLLIDTDRFKHINDLYGHSKGDEVLCALARTLESCARKGDLVFRWGGE
                     EFVLLLPRTPLDTALSLAETIRVSVAKVSISGLPRFTVSIGVAHHEGNESIDELFKRV
                     DDALYRAKNDGRNRVLAA"
     gene            complement(1387..1950)
                     /gene="smrA"
                     /locus_tag="ACHKBL_004456"
     CDS             complement(1387..1950)
                     /gene="smrA"
                     /locus_tag="ACHKBL_004456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
                     /protein_id="HFO5821213.1"
                     /translation="MNLDDKSLFLDAMEDVQPLKRATDVHWHPTRNQRAPQRIDTLQL
                     DNFLTTGFLDIIPLTQPLEFRREGLQHGVLDKLRSGKYPQQASLNLLRQPVEECRKMV
                     FSFIQQALADGLRNVLIIHGKGRDDKSHANIVRSYVARWLTEFDDVQAYCTALPHHGG
                     SGACYVALRKTAQAKQENWERHAKRSR"
     gene            complement(2145..3659)
                     /locus_tag="ACHKBL_004457"
     CDS             complement(2145..3659)
                     /locus_tag="ACHKBL_004457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020231236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HFO5821214.1"
                     /translation="MMSMRKHIAFASMCMGLFIAQLDIQIVSSSLNEIGGGLSAGKDE
                     MAWLQTSYLIAEIIVIPLSGWLSRVFSTRWLFTLSAGIFTLMSIACGLAWNIQIMILF
                     RALQGAAGASMIPLVFTTAFIYYQGKELGSAAAVVSALASLSPTLGPTLGGWITDNLD
                     WRWLFYINILPGIYLVLSIPFLVNFDKPDLSLLKVADYPSIILLAMTLGCLEYTLEEG
                     ARWGWLDDNTILLTSVLALVSFILFAARTLTISNPIMDLHAFKDKNFTLGCFFSFSGG
                     VVIFSTVYLILVFLGQVRGLNAEEIGFAVCTTGIFQLFSVPFYFWLSKKINLRWLLMA
                     GLGGFVFSMYLFTPITHEWGWQELLFPQAIRGISQQFAMAPIVTLTLGGIPKERLKLA
                     SGVFNLTRNLGGAIGIALCGSILNNRTNFHFSRMGEKMVSVPHTVNDFISRSALFFNR
                     SGSDQTSEILASTKLLSQLMLREAQTMAFSDTFLLISGLLFIAFLLVPAMNKSS"
     gene            complement(3656..>4675)
                     /locus_tag="ACHKBL_004458"
     CDS             complement(3656..>4675)
                     /locus_tag="ACHKBL_004458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFO5821215.1"
                     /translation="SKKQLIGVVAIGIILAGVVFFIWWVSKGRFIQTTDDAYIGGNIT
                     TVASKVSGYISAIEVRDNQSVKKGDIILRLDDRDYRANVARLEAKIKSRKANLESIQA
                     TITMQQSIIQSASETWQAVKHEEQKRLRDTERYEKLVQSAAISQQIIDNARFDYQQVA
                     AKERKAANDFLVEKQRLAVLSAQEENVRASIEEVQAALTQALLDLEYTLVRAPIDGIV
                     ANRSAHTGSWVEGGTSLVSLVPVSELWVDANYKENQIAGMKPGMKAEIRADILKGEVF
                     HGHIESLSPATGASFSLIPIENATGNFTKIVQRVPVRIAFDDAKELKQLLRPGLSVTV
                     SVDER"
BASE COUNT         1320 a         1172 c          985 g         1198 t
ORIGIN      
        1 caaaatgctt ttccatatac aaaccgtgtg aagtgttaaa tagcgtctat cattatcaga
       61 attatctgat catatgacgt ggcttttttg cgatcggata gcaacaaaaa ttgataaaaa
      121 taacgggatc tcaatgatta cgcacaactt caataccctg gacttactca ccagtcctgt
      181 ctggatcgtt tcgccctttg aggaacagtt aatttatgcc aacagcgcgg cgcgactgtt
      241 gatgcaagac ctcacgttta gtcagctacg aaccggtccc tattccgtct cctcacaaaa
      301 agaactgccg aaatacctct ccgatctgca aaaccaacac gatattatcg aaatcctcac
      361 cgttcagcgc aatgaagagg aaacagcatt gagctgtcgg cttgttttgc gaaagctgac
      421 agaagcagaa ccggtgatta ttttcgaagg tatcgaagca ccggcaacgc tgggtttaaa
      481 agccagtcgc tcggcaaatt atcagcgcaa aaaacaaggt ttttatgcgc gcttttttct
      541 gactaactct gcaccaatgt tgttgattga cccctcacga gatggccaaa tcgtcgatgc
      601 taacctcgcc gcgctcaatt tctatggcta taaccatgaa acgatgtgcc agaaacatac
      661 ctgggaaata aatatgctcg ggcgacgcgt catgcctatc atgcatgaaa tttcgcattt
      721 acccggtggc cataagcctt tgaattttgt tcataaactg gcggatggtt cgactcgtca
      781 tgtgcagacc tatgccggtc caattgaaat ttatggcgac aagctcatgt tatgtattgt
      841 gcatgatatt actgagcaaa aacggctgga ggagcagctg gaacatgctg cccaccatga
      901 tgcgatgacc ggattactga atcggcgaca gttttatcac attaccgagc caggccaaat
      961 gcagcacctc gccatcgctc aggattacag cttgttgctc atcgacaccg atcgttttaa
     1021 acacattaac gatctctatg ggcattctaa aggtgatgag gtgttatgcg cccttgcccg
     1081 cactctcgaa agttgcgctc gcaaaggcga tttggtgttt cgttggggag gcgaagagtt
     1141 tgtcttattg ctaccaagaa ccccactgga taccgcgctt tcgctggctg aaactatccg
     1201 cgtaagcgtg gcaaaagtga gtatttcggg cttaccacgc tttactgtca gcattggtgt
     1261 ggcgcaccac gaaggaaatg aaagcatcga tgaactgttt aaacgcgttg atgatgcttt
     1321 gtatcgagcg aaaaatgatg gacgcaaccg cgtgctggcg gcataagcca cggatgcgtc
     1381 tcgtgatcaa cgactgcgct tagcgtggcg ctcccagttt tcttgcttcg cctgcgctgt
     1441 tttacgtagt gcgacgtaac acgccccgct gccgccatga tgcggtagcg cagtgcaata
     1501 tgcctgaaca tcatcaaatt cggtcagcca gcgcgccaca tagctgcgga caatattggc
     1561 atgcgattta tcatcccgtc ctttgccatg aataatcagc acgttacgca aaccatccgc
     1621 cagggcttgt tgaatgaaac tgaacaccat tttgcggcac tcttccaccg gctggcgcaa
     1681 aagattcagg ctcgcctgtt gcggatattt accactgcgc agcttatcca gcaccccatg
     1741 ttgcaaccct tcccgccgaa actccagcgg ctgagttagc gggatgatgt cgagaaatcc
     1801 ggtggtgagg aaattatcaa gctgcagcgt gtcgatacgc tgcggcgcac gttggttacg
     1861 cgttggatgc cagtggacat ctgtagcacg tttcagcggc tggacatctt ccatggcgtc
     1921 aagaaacagc gatttgtcgt caaggttcat gtaacctcct atgcaccttc acacgtcgtg
     1981 attcagacgg gttaattgtt catacttact gcatcgttcc cttcacagat tgaaacaata
     2041 ctctacttca tacctgtgag tatctgcgca ggcggggcat tttatactag cctgttagca
     2101 gtatcgaaca gtttagcccg agtttaattc agcacaggct ccgatcagga tgatttattc
     2161 atagcaggaa ccaacaaaaa tgcgataaat aacagaccac tgattagcag aaaggtatcc
     2221 gaaaatgcca tagtttgcgc ctccctcagc atcaactgac tcaataattt ggttgaagcc
     2281 agtatttccg atgtctggtc acttccactg cgattaaaaa acaaagcact tctggaaatg
     2341 aagtcattca ccgtatgggg aacagaaacc attttttcac ccattctcga aaaatgaaaa
     2401 ttagttcgat tattgagaat agagccacac agggcaatac caatcgcgcc cccaagatta
     2461 cgtgttaaat taaatacacc agaggctaac ttcagccgtt cctttggaat tccccccagc
     2521 gttaatgtta cgatgggggc catcgcaaac tgctgcgaaa tccctctaat tgcctgtgga
     2581 aacagtaact cctgccatcc ccattcgtgg gttatagggg taaaaagata cattgaaaac
     2641 acaaatccac ccagccccgc catcaacagc catcgcagat ttattttttt acttaaccag
     2701 aagtaaaagg gaacagaaaa taactggaaa atacctgttg tgcaaacggc aaaacctatc
     2761 tcttcggcat ttaatccgcg aacctgcccc aaaaacacaa gtattaaata tacagtagag
     2821 aatatcacaa cccctccaga aaaagaaaaa aaacatccca gcgtgaaatt tttatcctta
     2881 aaagcatgaa gatccattat cggattggat attgtaagtg tacgcgcagc aaataaaata
     2941 aaactcacca atgccagaac tgatgtcagc aaaatagtat tatcatccag ccatccccag
     3001 cgcgctcctt cttcaagggt atattccaga catcccagtg tcatggccaa caggataata
     3061 ctcggataat cagcaacttt cagtaaagag agatcaggtt tatcaaaatt aaccagaaaa
     3121 ggaatactca aaactaaata tatgccaggt aatatattga tataaaatag ccatcgccag
     3181 tcgaggttat cagtgatcca gccccccaga gttggcccta gcgtcggtga cagtgaggcc
     3241 agcgcactga ctaccgcagc agccgacccc aactctttac cctggtaata aataaacgcc
     3301 gtcgtgaaaa ctaaagggat catggatgcg ccagccgctc cctgtaaagc acgaaacaaa
     3361 atcatgatct gaatattcca tgcaagtcca cacgcgatac tcattaaagt aaatattccc
     3421 gcggatagag taaaaagcca acgagtggaa aatacccgtg acagccaccc agagagcgga
     3481 ataacaataa tctcagcaat taaatagctg gtctgaagcc aggccatttc gtctttacct
     3541 gcggacagac caccacctat ttcatttagg gatgacgata caatttgaat atcaagctga
     3601 gcaataaata atcccataca catactggcg aaagcaatat gtttacgcat agacatcatc
     3661 tctcatcgac actgacggtg acggacagtc ccgggcgaag aagttgcttt aattcctttg
     3721 cgtcgtcaaa tgcaatccgt accgggacac gctgaacaat tttggtgaaa ttgccagtgg
     3781 cattttctat cggaatcaag ctgaaactgg ctcctgtcgc tggggaaagg ctttcaatgt
     3841 gaccgtgaaa cacttcccct ttgagaatat cagcacggat ctcagccttc attcctggct
     3901 tcattcccgc tatttggttt tctttataat ttgcatcaac ccacaactca ctgactggta
     3961 caagtgaaac taacgatgtg ccgccttcca cccaactgcc tgtatgggcg ctgcggtttg
     4021 cgacaatgcc atcaatcggc gcacgaacaa gggtatattc cagatccagt agtgcctgag
     4081 tcaaagcggc ctgaacttct tcgatactcg cacgtacgtt ttcttcctga gcagataaaa
     4141 ctgccagccg ttgcttctcc accaggaaat cgttcgcggc tttgcgctcc tttgctgcga
     4201 cctgctggta gtcaaacctg gcgttatcaa taatttgctg cgatattgcc gctgattgta
     4261 cgagtttttc ataacgttca gtatctcgca ggcgcttttg ctcctcatgt tttactgcct
     4321 gccatgtttc actggcactt tgaattatcg actgctgcat cgtaatagtg gcttgaatgc
     4381 tttcgagatt ggcttttcta ctttttattt ttgcctcaag cctggcgaca tttgcacgat
     4441 aatcccgatc atcgagacgc aggataatat cgcctttttt tacgctctga ttatcacgca
     4501 cctctatagc agaaatataa ccagaaactt tacttgccac cgtggtaata ttaccaccaa
     4561 tgtaagcatc atccgtcgtc tgtataaatc ttcctttgct tacccaccag ataaaaaaga
     4621 caacgccagc caaaataata ccgatcgcta ctaccccaat aagctgtttt ttaga
//
LOCUS       DAZFLD010000274         4638 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.276, whole genome shotgun sequence.
ACCESSION   DAZFLD010000274 DAZFLD010000000
VERSION     DAZFLD010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4638
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.276"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            39..755
                     /gene="nanC"
                     /locus_tag="ACHKBL_004459"
     CDS             39..755
                     /gene="nanC"
                     /locus_tag="ACHKBL_004459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid outer membrane channel
                     NanC"
                     /protein_id="HFO5821216.1"
                     /translation="MKKAKILSGVLLLCFSSPLISQAATLDVRGGYRSGSHAYETRLK
                     VSEGWQNGWWASMESNTWNTIHDNKKENAALNDVQVEVNYAIKLDDQWTVRPGMLTHF
                     SSNGTRYGPYVKLSWDATKDLNFGIRYRYDWKAYRQQDLSGDMSRDNVHRWDGYVTYH
                     INSDFTFAWQTTLYSKQNDYRYANHKKWATENAFVLQYHMTPDITPYIEYDYLDRQGV
                     YNGRDNLSENSYRIGVSFKL"
     gene            775..1881
                     /gene="nanM"
                     /locus_tag="ACHKBL_004460"
     CDS             775..1881
                     /gene="nanM"
                     /locus_tag="ACHKBL_004460"
                     /EC_number="5.1.3.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HFO5821217.1"
                     /translation="MNKTITALTIIMASFAANASVLPETPVPFKSGTGAIDNDTVYIG
                     LGSAGTAWYKLDTQAKDKKWTALAAFPGGPRDQATSAFIDGNLYVFGGIGKNSEGLTQ
                     VFNDVHKYNPKTNSWVKLMSHAPMGMAGHVTFVHNGKAYVTGGVNQNIFNGYFEDLNE
                     AGKDSTAIDKINAHYFDKKAEDYFFNKFLLSFDPSTQQWSYAGESPWYGTAGAAVVNK
                     GDKTWLINGEAKPGLRTDAVFELDFTGDNLKWNKLAPVASPDGVAGGFAGISNDSLIF
                     AGGAGFKGSRENYQNGKNYAHEGLKKSYSTDIHLWHNGKWDKSGELSQGRVYGVSLPW
                     NNSLLIIGGETAGGKAVTDSVLISVKDNKVTVQN"
     gene            1946..2926
                     /gene="nanS"
                     /locus_tag="ACHKBL_004461"
     CDS             1946..2926
                     /gene="nanS"
                     /locus_tag="ACHKBL_004461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HFO5821218.1"
                     /translation="MNAIISPDYYYVLTVAGQSNAMAYGEGLPLPDREDAPHPRIKQL
                     ARFAHTHPGGPSCHFNDIIPLTHCPHDVQDMQGYHHPLATNHQTQYGTVGQALHIARK
                     LLPFIPDNAGVLIVPCCRGGSAFTAGSEGTYSERHGASHDACRWGTDTPLYQDLVSRT
                     RAALAKNPQNKFLGVCWMQGEFDLMTCDYSSHPQHFNHMVEAFRRDLKQYHSQLNNIT
                     DAPWFCGDTTWYWKENFPHAYEAIYGNYQNNVLANIIFVDFQQQGERGLTNAPDEDPD
                     DLSTGYYGSAYRSPENWTTALRSSHFSAAARRGIISDRFVEAILRFWRER"
     gene            complement(2934..3584)
                     /locus_tag="ACHKBL_004462"
     CDS             complement(2934..3584)
                     /locus_tag="ACHKBL_004462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001586610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFO5821219.1"
                     /translation="MNKLPDHLPRRYIIEAIHLWDAGETHQFQPARLYEIEYHGRRYP
                     SKAIAGIAATLMTGTQFTPADFTGGIKSKCVKLLIEQGFHIHQDKAAAAPIADVLFPD
                     ELPSQETYLEGAAIKVTVNRYERDKKARDKAVEHHGCQCNVCGVDLVKIYGDIAEGFI
                     HMHHLVPLSAIKEDYQLDPVNDLLPVCPNCHAMLHRRKPPFSPEELKALMDANKSN"
     gene            3721..3864
                     /locus_tag="ACHKBL_004463"
                     /pseudo
     CDS             3721..3864
                     /locus_tag="ACHKBL_004463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_080685909.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
     gene            complement(4515..>4638)
                     /locus_tag="ACHKBL_004464"
     CDS             complement(4515..>4638)
                     /locus_tag="ACHKBL_004464"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFO5821220.1"
                     /translation="HLTLAVSNLQTSLTFWRDLLGLHVGDLASRLTQCRERPCS"
BASE COUNT         1332 a         1000 c         1102 g         1204 t
ORIGIN      
        1 ttgtattgct ttctgtatcc tacaaatgag tgaaatttat gaaaaaggct aaaatacttt
       61 ctggcgtatt attactgtgc ttttcgtcac cattaatttc tcaggctgcg acactggacg
      121 tacgtggtgg atatcgtagc ggaagccatg cctatgagac tcgactcaaa gtcagtgagg
      181 gatggcaaaa tggatggtgg gcaagcatgg aaagtaatac ctggaatacc attcatgata
      241 ataaaaagga aaatgccgca ctcaatgatg ttcaggttga agttaattac gcgattaaac
      301 ttgatgatca atggacggtg cgcccgggaa tgttaacgca ttttagcagc aacggcacac
      361 gctacggacc ctacgtaaaa ctgtcctggg acgcgacaaa agatcttaat tttggcattc
      421 gctatcgtta cgactggaaa gcttaccgac aacaagactt atccggtgat atgtctcgtg
      481 ataacgttca tcgttgggat ggatatgtca cttaccatat taatagtgat ttcaccttcg
      541 catggcaaac gacgctatac agcaaacaga atgattatcg ctatgcaaac cacaagaaat
      601 gggcgacgga aaatgccttt gttctacaat accatatgac gcccgatatt acgccataca
      661 tagaatatga ctaccttgac cgtcagggtg tttacaacgg cagagataat ttatcggaaa
      721 acagttatcg cattggtgtg tcatttaaac tgtagtagac aggagacagt cacaatgaat
      781 aaaacaataa cggcgcttac tatcataatg gcttcatttg ccgcaaacgc gtctgtatta
      841 ccggaaactc ctgtgccatt taaaagtggt accggagcaa ttgataacga cactgtctac
      901 attggtttag gtagcgcagg tacggcatgg tacaagctgg atacacaggc caaagataaa
      961 aaatggacag cgttagctgc attccctggc ggaccgagag atcaagcaac ctctgcattt
     1021 attgatggca atctgtatgt gtttggcggc attggcaaaa acagcgaggg cttgactcaa
     1081 gtatttaatg acgtacacaa atacaacccc aaaaccaata gctgggttaa attgatgtcg
     1141 cacgcgccga tgggcatggc gggccatgtg acttttgtac acaacggcaa ggcttatgtt
     1201 actggcggtg ttaaccagaa tatcttcaat ggctattttg aagatctcaa cgaagctgga
     1261 aaagattcaa ccgctataga taaaatcaac gcccactatt ttgacaaaaa agcagaagat
     1321 tatttcttca ataagtttct gttgtctttt gatccctcaa cacagcaatg gagttacgct
     1381 ggcgaatctc cctggtacgg aacggctggt gcggcggttg tgaataaagg tgataaaacc
     1441 tggcttatta atggcgaagc caaaccagga ttgcgaacgg atgccgtatt tgaacttgac
     1501 ttcaccggtg ataatttaaa atggaataag cttgctcccg tcgcatcacc agatggcgtc
     1561 gctggcggtt ttgcggggat aagcaatgat tctcttatat ttgccggagg ggccggattc
     1621 aaaggttcgc gggaaaatta ccaaaacggt aagaactatg cgcatgaagg cctgaaaaaa
     1681 tcatatagca ctgatattca tctttggcat aacgggaaat gggataaatc tggtgaatta
     1741 tcgcaaggtc gggtctacgg agtatcattg ccctggaata atagtttatt aattattggc
     1801 ggtgaaactg caggcggcaa agcggtgacg gattcagttt taatttctgt gaaggataat
     1861 aaagtcacag tacaaaacta acgcttcagg gcctcggtaa aggggccctg ctatcaattt
     1921 gtcatttgta ttacgcgaaa attatatgaa cgcaataata tcgcccgatt attactatgt
     1981 tcttaccgtt gctggtcagt ctaatgccat ggcgtatggc gaaggactgc cattaccgga
     2041 cagggaagat gcgcctcatc ccagaattaa acaattagcg agatttgcgc atacgcatcc
     2101 cggaggcccg tcatgtcact ttaacgacat tattccactg actcactgcc cacacgatgt
     2161 tcaggatatg cagggttatc accatcctct ggcaacaaat catcaaacac agtacggcac
     2221 cgttggccag gcactgcata ttgcacggaa attactgccc tttattcctg ataacgcagg
     2281 ggttctcatc gttccgtgtt gccgtggcgg atcggctttt accgcgggca gcgaagggac
     2341 atattcagaa cggcacggag ccagccatga tgcttgtcgt tggggaacgg atactccgct
     2401 gtaccaggat ttagtcagca gaacgcgagc cgcactggca aaaaatccgc agaacaaatt
     2461 cctcggcgta tgctggatgc aaggcgaatt tgacttaatg acctgtgact actcgtcaca
     2521 ccctcaacac tttaatcata tggttgaagc ctttcgtagg gatctaaaac aataccattc
     2581 tcagcttaat aatattactg acgcaccgtg gttttgcggc gataccacct ggtactggaa
     2641 agaaaatttc cctcatgcgt atgaagcgat ttatggcaat tatcaaaata atgttttagc
     2701 caatattatt ttcgtcgact tccagcaaca aggtgaaaga ggactgacga acgcgcctga
     2761 tgaagatccg gacgatttaa gcacgggata ttacggttca gcgtaccggt caccggagaa
     2821 ctggacgacg gcactgcgaa gcagtcattt cagcgcggca gcccgtcggg ggattatttc
     2881 tgacaggttt gtagaagcaa ttttgcggtt ttggcgcgaa aggtgagcgt tcattaattt
     2941 gatttatttg catccatcaa cgcttttaac tcctccggtg aaaacggtgg tttgcgtcgg
     3001 tgcagcatcg catggcaatt agggcacaca ggcagcaaat cattaactgg atccagctgg
     3061 taatcctctt taatggctga aagcggcacc aggtgatgca tatggataaa gccttcagcg
     3121 atatcaccat agatcttcac cagatcaacg ccgcatacgt tgcactggca tccatgatgt
     3181 tcgaccgctt tatctcgggc ctttttatcg cgttcatagc ggtttactgt cacctttatc
     3241 gccgcacctt caaggtatgt ctcctgcgac ggcaactcat ccgggaacag aacgtcggct
     3301 atgggagccg cagcggcttt atcctgatga atatgaaatc cctgctctat gagtagtttg
     3361 acgcatttag attttattcc gccggtgaaa tccgcagggg taaattgcgt ccccgtcatc
     3421 aatgtcgctg cgatgccagc aatcgccttc gaggggtaac gtcggccatg atattcaatc
     3481 tcatacaacc gcgcaggctg gaactgatga gtttctcccg catcccataa atggatagct
     3541 tcgatgatat accgccgcgg aagatgatca gggagtttat tcatggagaa tgtccttata
     3601 cgttctcaga acaggcagca aaaaaacaaa tgagatcaag gtaatatatc ttcacttgca
     3661 ggcttgaaca gcagaaaatt tgcgagcatg atcgcaatta tttcactgcc agggatatca
     3721 atggcttcca gtaaatcgct acagcaggca attgccaata taaaaatctg gcacaagggt
     3781 gaacagcgcg cgccgcataa gccattgtta ttgctatacg tattagcggg atacctgaat
     3841 ggacatccgc gccttttcga ttattgtagt ggaacagtct acgaggatgc agttcgaagt
     3901 gagtaagtaa aggttgctgg aaacgatcaa tgccgccata caatgatttt tgaggatcaa
     3961 caatgagtaa taaaaactac gaaagtcacc gcaaagcgat tgttagtaaa ggtataccac
     4021 ccgctctatt aaataggctc accaattcgg atgttcaggt gatcaatacc ttcttaacac
     4081 gagtgagcaa actggaacta tctcaacaag agaaagactg gatcataaaa atcatctcta
     4141 tggtttagac aatttcagcc ttgtagcctt tcaagggttt tgtgtgggta caaacattat
     4201 cgaatcggga tatagccgca gctgtagctg tatccacatc agtaagttga tgtaggttga
     4261 cttaggttga gcgaaaaata gcgaaaagcc ttgtgtggcg cggatttcag tcataaaaaa
     4321 acgtccgttg acgtctattg atgttccgat ggtgcgaagg tcgaaggccg gactcgcctt
     4381 tgcgtttaat tcgttgaaaa ataatggttt gtgagatttt attttgagga tttgccccca
     4441 aaatgggaca taaaattgaa tactgggtgg aagatcacaa agttgcgtaa ttattttcca
     4501 ggtccgaaac gcattcaaga gcaaggcctc tcccggcact gcgtcagtcg ggatgctaaa
     4561 tctcccacat gtagccctag taaatcgcgc cagaatgtca ggctagtttg caaattactg
     4621 acggcgagcg ttaagtgg
//
LOCUS       DAZFLD010000275         4587 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.277, whole genome shotgun sequence.
ACCESSION   DAZFLD010000275 DAZFLD010000000
VERSION     DAZFLD010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4587
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.277"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..362)
                     /locus_tag="ACHKBL_004465"
     CDS             complement(<1..362)
                     /locus_tag="ACHKBL_004465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HFO5821221.1"
                     /translation="MDISTLLLDFRITSHCNLGCDLCFRTPGIQDSSLASARNVIERM
                     YKMGFRRIGFTGGEPTSRSDFIDLIEYAKQLGFMTYLSTVGHRFIMDHARLNPVLDWV
                     GIPIDGIDSLVNSEIRSD"
     gene            847..1098
                     /locus_tag="ACHKBL_004466"
     CDS             847..1098
                     /locus_tag="ACHKBL_004466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001595120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFO5821222.1"
                     /translation="MPNIILSDISASVSELKKNPMATVGAGDGYPVAILNRNQPAFYC
                     VPAELYERMLDALDDQELVKLVTERSSQPLHDVDLDSYS"
     gene            1095..1382
                     /locus_tag="ACHKBL_004467"
     CDS             1095..1382
                     /locus_tag="ACHKBL_004467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020317119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO5821223.1"
                     /translation="MTYTIKFRDDALKEWLKLDKTIQQQFVKKLKKCSKNPHILSAKL
                     RGLKDCYKIKLRASGFRLVYQVIDDMLIIAVVAVGKRERSNVYYLASERMR"
     gene            1550..1954
                     /locus_tag="ACHKBL_004468"
     CDS             1550..1954
                     /locus_tag="ACHKBL_004468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016529521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2251 domain-containing protein"
                     /protein_id="HFO5821224.1"
                     /translation="MTILVTAQAQLIVGEAMAIKSLAPDGMLAAVFEDDGNTGYFYAL
                     DESVEGNPVRDAVHIYNVEDISDAHIPSDVKIGWSEDCLKCVLLINGYPHGVFDFEGK
                     NGYCRSGFPPPVNHEWSVQGHSWDDYVDNFFR"
     gene            2263..3747
                     /locus_tag="ACHKBL_004469"
     CDS             2263..3747
                     /locus_tag="ACHKBL_004469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFO5821225.1"
                     /translation="MRKIIAHFKIVLTLLLPATVSAQSIEWQSCMTTPFSNWFGEEKP
                     SPDLLCGYLSVPLKYTDTGGDSSSVKRQQVKLALTKLPAKSRHKGSIIIISGGPGLPG
                     INPYINFDWPVTNLRESWDIIGFDPRGVGQSIPAINCQQSDTGIQENITEKQRVLNKI
                     NSCIHNTGTEVIRHIGSNEAVYDIDRIRQALGDEQLTAVAYSYGTQIAALYAERFPSS
                     IRSIVLDGVVDIDDLGDNFTWQLKQAQSYQETFDRFASWCARTKSCPLSSVRDKAITQ
                     FHELLSKLHHKPLLDSKGENISSDELILLTTDLLLWRSSWPTLATAIRQFSQGIVSNE
                     IETALSTPIASEESSDALGVILCVDQGGEQLTPEERKSRKDALANAFPAINFDNGRSD
                     SPDFCELWPIHSNLNKTRLKNTVLPSGLLFVAHKYDPTTPWINARKMAEKFSSPLLTI
                     NGDGHTLALTGVNLCVDKAVVRHLITPMKMGNISCPGNAEIDIK"
     gene            complement(3968..4576)
                     /locus_tag="ACHKBL_004470"
     CDS             complement(3968..4576)
                     /locus_tag="ACHKBL_004470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ProQ/FINO family protein"
                     /protein_id="HFO5821226.1"
                     /translation="MGKDLEEKKNPVIVVKKRRSFSPPSLAEKKDVIAPALTEQATES
                     VSSGISSPAVETHIPEAPARKKKKKRQRFPRPSHWTSEFTHDCLEKVKALFPQLRAEG
                     GGFLPLKTGITRDITTYLAAHPETALTLDEWNCAVSCITSRRIYLQRTSVAGTPRYNL
                     DGQPEGEVSECDAQNSRRWLAAKEQRWLSMKARQENISEKSK"
BASE COUNT         1325 a          967 c         1019 g         1276 t
ORIGIN      
        1 ttatcactgc gaatttctga attgacaaga ctgtcaattc catcaattgg tattcctacc
       61 cagtctaaca ccggatttaa ccgagcatga tccataataa atcgatgccc gaccgtggag
      121 agatatgtca taaaacccag ttgcttagcg tattcaatca gatcaatgaa atcactccgg
      181 gatgtaggtt cccctccggt aaaaccgata cgacggaatc ccattttata catccgctca
      241 attacattac gagcactagc aagagaagag tcctgaattc caggtgtacg aaaacacaga
      301 tcacatccta ggttacaatg tgaagttatt ctgaaatcta aaagaagggt gctaatatcc
      361 ataacaagtc ctctgactat gatacatcaa taaccattaa aacataatgg ttattgcaga
      421 ccatgacaaa gcgcaacctt cggcttacca gggatataat atctgccagt aatcagagca
      481 gtgggttgcg caaggaactc atgaatgatg agttccggta atcgcattac tggaagccca
      541 tgatcggagg gacgctatca gtcacaccag tgaaaattgt gcacagaagc attttcatca
      601 ctgacaggcc gtatgcctca caagacggga gataactgga gaatatttag ttcttctacc
      661 ggcttacgcc gattttactc ctgtgttata tgtaaccatt gtgtgccacc ttccatgcgc
      721 tggtgcggca tcagagtaaa aaatcagcat taaatttatt tgcaaatgta tgagtaatac
      781 agacttgtta acgggtcctt tcagatgtat ctttaagact tattaaacag tcttaaaagg
      841 tgcattatgc ctaacatcat tctgtctgac atcagtgcaa gtgtcagtga actgaaaaaa
      901 aatccgatgg cgacagtcgg tgctggtgat ggttatccgg tcgccattct gaatcgcaat
      961 cagccggcat tctactgtgt tcctgctgaa ctgtatgaaa ggatgcttga tgctctggat
     1021 gaccaggagc tggtgaaact ggttactgaa cgtagcagtc aacctctgca tgatgttgac
     1081 ctggacagtt attcatgact tatacgataa aattcaggga tgatgcgctt aaagaatggc
     1141 tgaaactgga taagaccatt caacaacagt ttgtgaaaaa actaaaaaaa tgtagcaaaa
     1201 atcctcatat actgtctgca aaactacgtg ggctaaagga ctgctacaaa attaaacttc
     1261 gtgcatcggg atttcgtctg gtttatcagg tgattgatga tatgttaatt attgcggttg
     1321 ttgcggtagg taaacgagaa cgtagtaacg tttattatct cgccagtgag agaatgagat
     1381 agtaacataa atagcctttc ccgcagataa ctctcagaat tgttattctc tctgtggcct
     1441 cgcctgaggc ggaaaaagaa aagttttgtt gagagaatga ttttacctgc cgggatgagt
     1501 ctggggttat ttatattacc agacaaattg aaacggaggg acatgtttga tgacaatact
     1561 tgtgacggca caggctcaac tcatagttgg tgaggctatg gcgataaaaa gtttagctcc
     1621 tgatggaatg ttggccgctg tatttgaaga cgacgggaat accggatatt tctatgcact
     1681 tgatgagtcc gttgaaggta accctgtcag ggatgctgtg catatatata acgttgaaga
     1741 catttcagat gctcacatcc cctctgatgt gaagattggc tggtctgagg attgcctgaa
     1801 gtgtgttctg ctgataaacg gatatccaca tggggtattc gattttgagg ggaagaacgg
     1861 ctactgtcga agtggattcc cgccaccggt taatcatgaa tggtctgttc aggggcattc
     1921 atgggatgat tacgtcgata atttcttccg gtgattgaac cgaaaaatta cctgacggac
     1981 aagtctgggg ccgaatcgtg atgattcatc atggccgttt atagttcagc agtcttgttc
     2041 ttttgctgtc agtgggatac caggaaaata accgattgtt accagacaga ataaccatca
     2101 taagacgtca caagacggtg acgcttacat agttccgggg agttaaacaa tataccctga
     2161 ctttgtaatc atttgaatta tatccgtaac gtgtaacttc ctgccgtaac accggattta
     2221 agatattcaa actttatctt tataccgata ttaagagaca caatgcgaaa aataattgct
     2281 catttcaaga tagttttaac cttacttctg cccgcaacag tatctgccca gtcgatagag
     2341 tggcaatcct gtatgaccac tccattcagt aactggtttg gtgaggaaaa accgtctcct
     2401 gacttactat gtggttattt gtctgttcca ttaaaatata cagacacagg cggagatagt
     2461 tcttctgtaa aaagacagca ggtaaaactg gcgctgacaa aattgccagc aaaaagcagg
     2521 cataaaggga gtatcattat aataagtggt ggaccaggat tacctggcat taatccttat
     2581 attaactttg actggccggt aacaaatctt cgcgaatcat gggatattat tggctttgat
     2641 ccacggggag tcggccagtc cattcctgca ataaactgcc agcaatcaga tacagggatt
     2701 caggaaaaca taactgaaaa gcaacgggta ttaaataaaa ttaacagctg tatccataat
     2761 accggaactg aagttattcg ccatattggt tctaacgagg cggtatatga tattgatcgt
     2821 atcaggcaag ccctgggtga cgagcaactg acagctgtgg catattcgta tggaacacaa
     2881 attgcagcct tatatgcaga acgtttccca tccagcatca gatctatcgt tcttgatggg
     2941 gtcgtcgata ttgatgacct gggggacaac ttcacatggc aactcaaaca agcacagagt
     3001 tatcaggaaa cgttcgatcg ctttgcatcc tggtgtgcac gtacaaaaag ttgcccactt
     3061 tcttcagtca gagataaggc aataactcag ttccatgagc tgttatcaaa attgcatcac
     3121 aagcctttat tagacagcaa gggagaaaat atatcttcag atgaactcat attattaaca
     3181 acagaccttt tgttatggcg ttcatcatgg ccaacccttg caactgccat acgtcagttc
     3241 tctcagggga ttgtcagtaa tgaaattgaa actgcgctca gtactcctat agcctcagaa
     3301 gagtcaagtg atgctctggg ggtaatcctt tgtgttgatc agggggggga gcaattaacc
     3361 ccagaagagc gaaaatcccg aaaagatgct cttgcgaatg ccttcccggc tattaacttt
     3421 gacaatggac gttccgattc acctgatttt tgtgaattat ggccaataca tagcaacctg
     3481 aataaaaccc ggctgaaaaa tacagttctg ccctctggtt tactgtttgt agcacacaaa
     3541 tacgatccaa caacaccatg gattaatgct cgtaagatgg cagagaaatt ttccagcccg
     3601 ttgctgacaa taaatggtga tgggcataca ttagctctta ccggagtcaa tttatgtgta
     3661 gataaggccg ttgttcgtca tcttatcacc cctatgaaaa tgggaaatat atcctgtcca
     3721 ggaaatgctg aaatagatat aaaatagttt aaaaattgcc cgccatttgc tcctgatacc
     3781 ggacatattt tgcattggga gcaaaatgcg ttcagtgcga tcacggcacc agaaacgtca
     3841 aactggatac acgtaaaaaa tgtcgcgatc gcgcaaaatc aagccattac tctcgggagt
     3901 gcttaaataa tacgcacaat atacaacgct ctcttctcta cagtctgttt cctgtaatat
     3961 cctgcggtta ttttgatttc tcagaaatat tttcctgccg ggccttcata ctcagccacc
     4021 tctgctcttt cgccgccagc cagcgcctgg aattttgagc atcacattcc gatacttccc
     4081 cctctggttg accatccagg ttataacggg gagttcctgc aacagaggtt ctctgcaggt
     4141 agatccggcg actggtaatg caggatactg cacaattcca ttcgtccagg gtcagcgctg
     4201 tttccggatg tgctgccaga tatgtagtaa tgtcacgggt tatgcctgtt ttcagaggca
     4261 gaaaaccgcc cccttcggca cgaagttgag gaaacagcgc tttcaccttc tccagacaat
     4321 cgtgagtgaa ctcactggtc cagtgggatg gtcttggaaa gcgttgtctt ttctttttct
     4381 tcttcctggc tggcgcttcg gggatatgag tttcgacggc aggtgaactg attccggagg
     4441 agactgattc tgtcgcctgt tcagtgagtg caggcgcgat aacatctttt ttttcggcta
     4501 aggaaggcgg tgaaaaagag cgacgttttt tcaccacaat gacgggattt ttcttttctt
     4561 ccagatcttt tcccataaat acctctg
//
LOCUS       DAZFLD010000276         4566 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.278, whole genome shotgun sequence.
ACCESSION   DAZFLD010000276 DAZFLD010000000
VERSION     DAZFLD010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4566
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.278"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            129..839
                     /locus_tag="ACHKBL_004471"
     CDS             129..839
                     /locus_tag="ACHKBL_004471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFO5821227.1"
                     /translation="MQTTRPPYSVSFMATVLLLLLFACHSTVANAAVALGATRVIYPA
                     NQKQVLLPVTNNDPASVYLIQSWIENAGDQKDTQFVITPPLFSMQGKKENTLRIINAT
                     NHQLPGDRESLFWVNVKAIPAMEKDQKNENTLQLAIISRIKMFYRPTNLAMAPEEAPA
                     MLRFRRSGSKLTLINPTPYFITVTNMKAGNSNLPNTMVPPKGEVSVDIPHAASGDISF
                     QTINDYGALTPRIKATMQ"
     gene            881..3532
                     /locus_tag="ACHKBL_004472"
     CDS             881..3532
                     /locus_tag="ACHKBL_004472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFO5821228.1"
                     /translation="MTAFRAAFKAYRMHQVLLLPRFARLTIALGLATAVFPVDAEYYF
                     NPRFLSNDLAESVDLSAFTKGHEAPPGTYRVDIYLNDEFMTSRDITFIADDNNADLIP
                     CLSTDLLVSLGIKKSALLDNKEHSAEKHVSDNSACTPLRDRLADASSEFNVGQQHLSL
                     SVPQIYVGRMARGYVSPDLWEEGINAGLLNYSFNGNSINNRSNHNAGKSNYAYLNLQS
                     GINIGSWRLRDNSTWSYNSGSSNSSDSNKWQHINTSAERDIIPLRSRLTVGDSYTDGD
                     IFDSVNFRGLKINSTEAMLPDSQHGFAPVIHGIARGTAQVSVKQNGYDVYQTTVPPGP
                     FTIDDINSAANGGDLQVTIKDADGSIQTLYVPYSSVPVLQRAGYTRYALAMGEYRSGN
                     NLQSSPKFIQGSLMHGLEGNWTPYGGMQIAEEYQAFNLGIGKDLGLFGAFSFDITQAN
                     TTLADDTRHSGQSVKSVYSKSFYQTGTNIQVAGYRYSTQGFYNLSDSAYSRMSGYTVK
                     PPTGDSNEQTLFIDYFNLFYSKRGQEQISISQQLGHYGTTFFSASRQSYWNTSRSDQQ
                     ISFGLNVPFGDITTSLNYSYSNNIWQNDRDHLLAFTLNVPFSHWMRTDSQSAFRNSNA
                     SYSMSNDLKGGMTNLSGVYGTLLPDNNLNYSVQVGNTQGGNTSSGTSGYSSLNYRGAY
                     GNTNVGYSRSGDSSQIYYGMSGGIIAHADGITFGQPLGDTMVLVKAPGADNVKIENQT
                     GIHTDWRGYAILPFATEYRENRVALNANSLADNVELDETVVTVIPTHGAIARATFNAQ
                     IGGKVLMTLKYGNKSVPFGAIVTHGENKNGSIVAENGQVYLTGLPQSGKLQVSWGKDK
                     NSNCIVEYKLPAVSPETLLNQQTAICR"
     gene            3546..4076
                     /locus_tag="ACHKBL_004473"
     CDS             3546..4076
                     /locus_tag="ACHKBL_004473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO5821229.1"
                     /translation="MKYNNVIFLGLCLGLTTYSALSADSVIKISGRVLDYGCTVSSDS
                     LNFTVDLQKNSARQFPTTGSTSPAVPFQITLSECSKGTTGVRVAFNGIEDAENNTLLK
                     LDEGSNTASGLGIEILDGNMRPVKLNDLHAGMQWIPLVPEQNNILPYSARLKSTQKSV
                     NPGLVRASATFTLEFQ"
     gene            4089..>4566
                     /locus_tag="ACHKBL_004474"
     CDS             4089..>4566
                     /locus_tag="ACHKBL_004474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO5821230.1"
                     /translation="MKRLHKRFLLATFCALFTATLQAADVTITVNGRVVAKPCTIQTK
                     EANVNLGDLYTRNLQQPGSASGWHNITLSLTDCPVETSAVTAIVTGSTDNTGYYKNEG
                     TAENIQIELRDDQDATLKNGDSKTVIVDEITRNAQFPLKARAITVNGNASQGTIEAL"
BASE COUNT         1310 a         1014 c         1021 g         1221 t
ORIGIN      
        1 accaacattt atttcaaacg cattaagtct ggaaacccgg cgactcgcca atgtattagc
       61 aacatttacc gattgtctga tgtaacggtt tccccgctta tcctcttaac acatggagaa
      121 tgcatcagat gcaaactacg cgtcccccct actctgtttc ctttatggcg acagtgttac
      181 ttttattgct ctttgcttgc catagcaccg ttgccaacgc agctgtcgca ctgggtgcga
      241 cgcgggtaat ttatccggca aaccagaaac aggttttatt gcctgtcacg aataatgatc
      301 ctgccagtgt ctatctcatt cagtcatgga tagaaaatgc gggtgaccaa aaagacaccc
      361 agtttgtcat tacgccaccg cttttttcca tgcaaggtaa aaaagagaac acgctgcgga
      421 taatcaacgc cactaatcat cagttaccag gcgaccgtga aagcctgttc tgggtgaatg
      481 ttaaagctat tcctgctatg gaaaaagacc agaagaatga gaatacatta cagctggcaa
      541 ttattagccg cataaaaatg ttttatcgcc ccacgaacct ggcaatggca cctgaagaag
      601 cgcctgcaat gcttcggttt cgtcgcagcg gtagcaaact tactcttatt aatccaacgc
      661 cctacttcat taccgtaacg aatatgaaag caggaaatag caatctccca aacaccatgg
      721 tgccgccaaa aggtgaagta tcggtggata tcccgcatgc cgcatccgga gatatcagtt
      781 ttcagacgat caatgattac ggtgcattaa ctccgcgtat taaggcaacc atgcagtaaa
      841 gcagtgactt aattaataat aaactcaatc ggaattaata atgacagcct ttcgtgccgc
      901 atttaaggca tatcgtatgc atcaggtgtt actactgcct cgctttgcac ggcttaccat
      961 tgcccttggc ttagcaactg cggtttttcc cgttgacgca gagtattatt ttaatccacg
     1021 gtttttaagc aatgatcttg ctgaatctgt cgatttatcc gcctttacta agggacacga
     1081 ggcaccacct ggtacatatc gggtcgacat ttatttgaac gacgaattta tgactagtcg
     1141 ggatattact tttattgcag atgataataa cgcagatctc atcccatgcc tgagtacaga
     1201 tcttcttgtc agtcttggca taaaaaaatc tgcattatta gacaataagg aacattcggc
     1261 agaaaaacat gtgtcggaca acagcgcatg cacaccactt cgggatcgtt tggctgacgc
     1321 atcatctgag tttaatgttg gtcagcaaca tctatcactc tccgttccgc aaatttatgt
     1381 tggcagaatg gctcgcggct atgtttctcc ggatctgtgg gaagaaggaa taaatgctgg
     1441 gctactaaac tatagtttta atggtaattc tattaataat cgtagtaacc ataatgcagg
     1501 aaaatccaac tatgcatatt tgaatttaca gagtggcatc aacattggta gttggcgact
     1561 gcgcgataac tcaacgtgga gttataacag tgggagtagc aattcatctg acagcaataa
     1621 atggcagcat atcaatacgt cggctgaacg tgacattatt cccttacgct cacgcttaac
     1681 ggtaggtgat agttataccg atggcgatat ttttgatagt gtaaactttc gtggcctcaa
     1741 aataaattca acagaagcga tgttgcccga tagccaacat ggtttcgctc cggtgattca
     1801 tggtattgcc cgcggcaccg cacaagtgag tgtaaaacag aatggatacg atgtttatca
     1861 gactactgtc ccacccggcc cttttactat tgatgatatc aactctgcgg ccaatggtgg
     1921 tgatctgcaa gtaaccataa aagatgcaga cggcagtatt cagacattat atgttcctta
     1981 ttcgtctgtt ccggttctcc aacgtgctgg atatacgcgt tatgcgcttg ccatggggga
     2041 atatcgtagt ggaaataacc tgcaaagctc ccccaaattc atacaaggta gcttgatgca
     2101 tggactggaa ggaaactgga caccttatgg cggaatgcaa attgcagaag aatatcaggc
     2161 cttcaacctt ggtattggta aagatttagg actttttggt gccttttctt tcgatatcac
     2221 gcaggccaat acgacgcttg cagatgacac ccgtcacagc ggacaatcgg ttaaatccgt
     2281 ctacagcaaa tccttctacc agacgggaac caatatccag gtcgcaggat atcgctattc
     2341 tacgcaaggt ttttataact tatccgacag tgcctacagt cgaatgagtg gttacaccgt
     2401 caagcctcct accggagaca gcaatgagca gacactattt attgattatt ttaatctgtt
     2461 ttacagtaag cgtggtcagg aacaaataag catctctcag cagcttggac attacggtac
     2521 gacatttttc agtgccagtc gccaaagtta ctggaacacg tcacgcagcg accagcaaat
     2581 atcatttgga ttaaatgtgc cgtttggtga tattacgact tcgctgaatt acagctattc
     2641 caataatata tggcaaaacg atcgggatca tttacttgct tttacgctta atgttccctt
     2701 cagtcattgg atgcgtacag acagtcagtc ggcatttcgt aattcaaacg ccagttacag
     2761 tatgtcaaac gatttgaaag gcggcatgac caacctatca ggggtttatg gcactctgct
     2821 gccggataat aacctgaatt atagcgttca ggtcggtaac acccaagggg gtaatacatc
     2881 gtctggcacc agtggttaca gttctcttaa ttatcgtgga gcttacggta atacgaatgt
     2941 cggttacagt cggagtggtg acagcagcca gatttattac ggaatgagtg gtggcattat
     3001 tgctcatgct gatggcatca cctttggaca gccgctgggc gacacaatgg ttctggttaa
     3061 ggctcctggc gctgataatg tcaaaataga gaaccagacc ggaattcata ccgactggcg
     3121 tggctatgcc atattaccat ttgcgacaga atatagagaa aatcgtgtcg ctcttaacgc
     3181 gaattccctt gcagataatg ttgaactgga tgaaaccgtt gtcactgtca tcccaactca
     3241 cggtgctatt gccagagcaa catttaatgc acaaatcggc gggaaagtat taatgacgtt
     3301 gaagtacggt aataagagcg ttccattcgg tgcaattgtc acacatgggg agaataaaaa
     3361 tggcagcatt gtcgcggaaa acggtcaggt ttatctgact ggacttccac agtcagggaa
     3421 attacaggtt tcatggggca aagataaaaa ctcaaactgt attgtcgagt acaagcttcc
     3481 tgcagtctct cctgaaacct tgctgaacca gcagacagca atctgtcgct aaggaacaat
     3541 agattatgaa atacaataac gttattttcc tcggtttatg tctggggtta accacctatt
     3601 ctgctttatc tgcagatagc gttattaaaa ttagcgggcg cgttctcgat tatggctgca
     3661 cagtctcatc ggattcgctt aattttaccg tagatctcca aaaaaacagt gccagacaat
     3721 ttccaacgac cggtagcaca agtccagccg tcccttttca gattacgtta agtgaatgca
     3781 gtaaagggac aacgggggtt cgggttgcat ttaacggtat tgaggatgca gaaaataata
     3841 ctctgttgaa actggatgaa ggaagcaaca cggcctccgg tttgggtata gaaatactgg
     3901 acggaaatat gcgtccggtg aaattgaatg accttcatgc cgggatgcag tggatcccac
     3961 tggtaccaga acagaacaat attttgcctt actccgctcg tctgaagtca actcagaagt
     4021 ccgtcaatcc gggactggtg agggcttcgg caacctttac ccttgaattt caataatcag
     4081 agccagatat gaaaagactt cacaagaggt tcctgttagc gacgttttgc gcgttattca
     4141 cagcaactct ccaggccgcc gatgtcacta tcactgttaa tggtcgggta gtcgctaaac
     4201 cctgcactat tcaaaccaaa gaagctaacg ttaatctcgg ggatctttat acgcgcaatc
     4261 tgcaacaacc tggttctgca tcaggctggc acaatattac tttgtcatta accgattgtc
     4321 cggttgaaac aagtgcagtg acggcaatcg tgacaggttc aactgacaat acgggttatt
     4381 acaaaaatga aggtactgcc gaaaatattc agatagagct aagggatgac caggatgcga
     4441 cgttaaaaaa tggcgatagc aaaacggtta ttgttgatga gatcactcgt aatgcacagt
     4501 ttccacttaa ggcaagagct atcacggtga atggaaacgc aagccaggga acgatcgagg
     4561 cgctaa
//
LOCUS       DAZFLD010000277         4528 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.279, whole genome shotgun sequence.
ACCESSION   DAZFLD010000277 DAZFLD010000000
VERSION     DAZFLD010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4528
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.279"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(273..596)
                     /locus_tag="ACHKBL_004475"
     CDS             complement(273..596)
                     /locus_tag="ACHKBL_004475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000866645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5821231.1"
                     /translation="MLTFKNGTVCVGDIFVSSWGYEQTNVNFYQVISVHGKKTVTVRE
                     ICASVHFTGNMMGYKRPLLNDFRGEPLKRRVLDCYRVPVIVIEEFEIAYKTLPEEEHE
                     FTSYS"
     gene            complement(707..934)
                     /locus_tag="ACHKBL_004476"
     CDS             complement(707..934)
                     /locus_tag="ACHKBL_004476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO5821232.1"
                     /translation="MEIENTPENIKRLRKKIGLTQKECAEMFGMTARSWRRKEEPTGT
                     VSGSKLSPVEFEYILLLAGEHPEYVLSKRDA"
     gene            complement(924..1184)
                     /locus_tag="ACHKBL_004477"
     CDS             complement(924..1184)
                     /locus_tag="ACHKBL_004477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020314676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5821233.1"
                     /translation="MKMRKGDRQYYLNKEGDTFRLVKRVKTFSKSATLGKTKATVKTV
                     ADIIFREEEFGSIDFTQDGLREGDKEIISIMLKNIKRDENGN"
     gene            complement(1375..3636)
                     /locus_tag="ACHKBL_004478"
     CDS             complement(1375..3636)
                     /locus_tag="ACHKBL_004478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HFO5821234.1"
                     /translation="MRLWIAEKPELGKLIAQVLGNPQYFDGYIVCNNDTVSWAIGHLL
                     KLIPPELKTPEYKSWSKNTLPLQLRPLQYIVIPKTEKQFNVLSGLITRADMIIHAGDP
                     DEEGQLLIEEILSYCGNKAPVKRVLINDLNKDAVRQAMRNLQDNRAFYGLYQKALARS
                     AGDYLFGLNMTRAYTIAAREKGHNEILSVGRVQTPVLGLVVRRYEENRDYKKYDYWCV
                     QAEFIDTDTIISPMLVPPEDAPVDDKGRIISEQYCDEVIRSCKGQQASVSLAESEIKS
                     RRPPLPFSLLDLQVKMNRQYGFTAEKVLEITQKLREEYRAITYNRSDCRYLSEEQFSQ
                     SPATLAMLSSFFPELGKIFSHIDSGRKSRAFNDKKISAHTAIIPTPTPTGLDQMNEDE
                     MRVYKAIVAQYLVQFMPDKTFDSAVVEFNINGFLFRQRAIRPVSPGWSILLADRDEET
                     LPDCKLFDAITSLTKGQQLVCSSVNSRKETSTPPPLYTEASLLEDLRRVARYVEDPRI
                     RQLLLDRDEGKEEERGGIGTPATRGHILNVLQVRGFIAVKNKKVVPTELGLSFIHALP
                     EVMTRPDMTALWHEQQSMIETGEISIDDFLDELEQFILQQVSDVDISGLAVAEYQCTC
                     GGRYVRRRTDKGFFWGCSKYPDCRNTVPDRGGVPDFSRSEFERKVRCPVCGSGMKISP
                     KAYNCSNERCGFRLWGTQFGKELTLAQATDLLMKGHTREIKGLKKKDGAKFDAVLNLN
                     RDGSVVPTFKKKK"
     gene            complement(3677..3910)
                     /locus_tag="ACHKBL_004479"
     CDS             complement(3677..3910)
                     /locus_tag="ACHKBL_004479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013087354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha/YmoA family nucleoid-associated regulatory
                     protein"
                     /protein_id="HFO5821235.1"
                     /translation="MENKYRKYPKSSKQEYLFQLRKCSKLSTLDKIIQRKAYTMNDID
                     FVEFLTAADHRLAEIVTGQLFDKIPKNVWKLIY"
     gene            complement(3960..4316)
                     /gene="ssb"
                     /locus_tag="ACHKBL_004480"
                     /pseudo
     CDS             complement(3960..4316)
                     /gene="ssb"
                     /locus_tag="ACHKBL_004480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016191742.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
     gene            complement(4328..>4528)
                     /locus_tag="ACHKBL_004481"
     CDS             complement(4328..>4528)
                     /locus_tag="ACHKBL_004481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFO5821236.1"
                     /translation="EIINISIGMIRGTKKELSTEIIKNARAFLKGSLDLSIQEAEEDI
                     DEREDIYRQNGAQPDYLSYLED"
BASE COUNT         1245 a         1062 c          720 g         1501 t
ORIGIN      
        1 gcttggtgtt tccttttcta tcaggttatt acatcttctt ttaagttttt tactctttat
       61 ttctgttttt tcagatattg caccaaaaat taattcagta tatccaacaa catttacttt
      121 cttcattttt aatctcctgc cgctgtgcgg ctctggtgat tggggccatc ccctctcgat
      181 gtattaatag taggtcataa acgacctatt tgcaagagtt ttttctcatt tttaaggaat
      241 aaaatagcag gagttccccc ctgctataat ccttaagaat atgaagtaaa ttcgtgttct
      301 tcttctggta aagttttata cgctatttcg aattcttcaa ttacaattac tggaacacga
      361 taacaatcaa gaactcgacg ttttagtggc tctcctctga aatcattcaa caacggacgt
      421 ttatacccca tcatatttcc ggtaaagtga acactggcac aaatctcacg aacagtcacc
      481 gttttttttc cgtgaacaga aatgacctga tagaaattaa cattcgtttg ttcgtatccc
      541 catgaagaca caaatatgtc accaacacaa actgtaccat ttttaaaagt taacattttt
      601 aatctcctgc cgctgtgcgg ctttggtgat tggggaccat cccctttcga tgtattaata
      661 ataggtcata aacgacctat ttgcaagtta tttttcttct tttttgctag gcatctcgtt
      721 tagaaaggac gtattcagga tgctccccag ccagtaacag aatgtattca aactctacag
      781 gtgaaagttt tgatccgctt accgttccag tgggttcttc tttgcgccgc catgaacggg
      841 ctgtcatccc gaacatctct gcacattctt tctgcgttaa tccaattttc tttcgcagac
      901 gcttgatatt ttctggtgta ttttcaattt ccattttcat ctctttttat gtttttaagc
      961 ataatagaaa ttatttcttt atcgccctct cttaagccgt cttgagtaaa atcaatagag
     1021 ccaaactctt cttcacgaaa aataatgtct gccactgttt ttaccgttgc ttttgttttc
     1081 cctaaagtcg cagacttaga aaaagttttt acccgcttca ccagacgaaa cgtatcacct
     1141 tctttattca gataatattg cctgtcacct tttctcattt tcatatctat atgattctct
     1201 ctaagagaag cctcaagcgg agccgaacga ctcgtacact gtacgcatgg tttccctatt
     1261 gcaacagatt taacatactt ttccatcttc aaactcctgc cgctgtgcgg ctttggtgac
     1321 agagaactat cccttctcga tgtaacggta ggtcataatc gacctgtttg caagttattt
     1381 tttcttttta aatgtcggta caacgcttcc atcacggtta agattcagca cggcatcaaa
     1441 cttagcacca tcttttttct taagaccttt aatttcccgc gtatggcctt tcatcaacaa
     1501 atctgtagcc tgtgctaagg tcagttcttt accaaactga gtaccccaca atcggaatcc
     1561 acatcgttca tttgaacagt tataggcttt tggtgatatc ttcatcccgg aaccacatac
     1621 cgggcatctg acctttctct caaactcgct acgcgaaaaa tcaggcacgc cgcctctgtc
     1681 tggaacagta ttccgacaat caggatactt actgcatccc caaaaaaaac ctttgtcggt
     1741 tctccgacga acataacggc caccacacgt acactgatac tctgctacag ccaggccaga
     1801 aatatccaca tcagacacct gttgcaaaat aaactgttca agttcatcaa gaaaatcgtc
     1861 gatactgatt tcccctgtct caatcataga ttgctgctca tgccatagag ccgtcatatc
     1921 tggtcgcgtc atgacctccg gtaaagcatg aataaacgat aagcctaact ctgtaggaac
     1981 cactttttta ttcttgacag caataaaacc gcgaacctga agtacgttaa gaatatgccc
     2041 ccttgttgcc ggtgttccaa ttccccctcg ttcctcctct ttaccttcgt ctctgtccag
     2101 taaaagttgt ctaatcctgg gatcttcgac ataacgggcc acacgccgta gatcctcaag
     2161 caatgatgct tctgtatata atggtggcgg tgtggatgtc tcttttcgag aattcacact
     2221 actgcaaacc aattgttgac cttttgtaag gctggttatg gcatcaaata acttacagtc
     2281 cggcaaggtt tcttcatctc tgtctgctag taatatactc cagccagggg aaacaggccg
     2341 tatcgctcgt tgccggaaca gaaatccatt gatattaaat tcaactaccg cagaatcaaa
     2401 agttttatca ggcataaact gcacaagata ctgtgccaca attgccttat aaaccctcat
     2461 ttcatcttcg ttcatttgat ccagcccggt aggcgttggt gtcggaataa ttgctgtatg
     2521 agcacttatt ttcttatcat taaatgccct gctttttcgc ccactatcta tatgagaaaa
     2581 aatttttccc agttcaggaa aaaaagaaga aagcattgct aatgtcgccg gagactgaga
     2641 aaactgttct tctgagagat aacggcaatc cgaacggtta taagtgatag ccctgtattc
     2701 ctcccttaat ttttgcgtta tctccaacac tttttcagca gtgaatccat actgacgatt
     2761 cattttcacc tgaagatcaa gaagcgaaaa tggtaatggt ggtctcctgc ttttaatttc
     2821 actctctgca agagaaacac tcgcttgttg tcccttacaa ctacgaatta cttcatcaca
     2881 atattgctcg ctgataattc tacctttatc gtccacaggt gcatcttccg gtggaacaag
     2941 catcggtgaa attattgtat cagtatcaat aaattcggcc tgaacacacc aataatcata
     3001 tttcttataa tccctgtttt cttcataccg ccgaacgacc aggccaagaa ctggtgtctg
     3061 aacccgtcct actgaaagta tttcattatg ccctttttct ctggctgcaa tagtatatgc
     3121 tctggtcata tttaaaccaa aaagataatc acctgcacta cgggcaagag ctttctgata
     3181 aagcccgtaa aacgctctgt tatcctgtaa attacgcatc gcctgacgca ctgcatcttt
     3241 attaagatca ttaatcagaa cccgtttaac gggagcttta tttccacaat aagaaagtat
     3301 ctcttcaata agtaactgcc cttcctcatc tggatcccct gcatgaataa tcatatctgc
     3361 acgagtaatt aatccagaca aaacattaaa ttgtttctct gtttttggga taacgatata
     3421 ttgcaacggt cgtaattgca gtggtaatgt atttttagac cagcttttat attcaggagt
     3481 tttcaattca ggaggtatta gctttaacaa atgaccaata gcccaagaaa ccgtatcatt
     3541 gttgcaaact atatacccat caaaatactg tgggtttccc agaacctgag caataagctt
     3601 cccaagctct ggtttttcag ctatccagag tcgcataaga atatctccgc ttgaaaagta
     3661 cgactggatg tatgatttaa taaatcagct tccagacatt tttaggaatt ttatcaaata
     3721 attgtccagt tacgatttca gccaaccggt gatctgcggc tgtcaaaaac tctacaaaat
     3781 caatatcatt cattgtatat gccttacgct gaataatttt atcaagagta gaaagctttg
     3841 agcacttcct gagctgaaac aaatactcct gcttactact ttttgggtac ttcctgtatt
     3901 tgttctccat ttaagttatt cctcaaacgg actcaatatc ttcaagactg tcctgttttt
     3961 gatttagtat tattaccaag catttgcata gttcccttct gatcaacaac aatctctgtt
     4021 gtataatgct cctccccatc tttatctgtc cattttctgt aacgtaatgt tccctcaata
     4081 tagacctgca ttcctttaca taaaaaatct ctggatatct ctgccagttt gttataaata
     4141 acaactcgat gccattctgt attctctttt atttcaccag actgtttatc tctccacgtt
     4201 tcgcttgttg ctatacttaa attagcaacg gtaactccat tctgcataca ccgaatctca
     4261 ggatcttgtc caagattacc tattaatgta actttattta tacctttagc agccatttaa
     4321 taccccacta atcctcaaga taacttaaat aatctggttg tgccccgttc tgtctgtata
     4381 tatcttccct ttcatcaata tcctcctccg cttcctgtat acttagatca agagatcctt
     4441 ttaaaaatgc acgagcattt ttaattattt ctgttgataa ctctttttta gttccgcgaa
     4501 tcattccaat tgatatattt attatttc
//
LOCUS       DAZFLD010000278         4508 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.280, whole genome shotgun sequence.
ACCESSION   DAZFLD010000278 DAZFLD010000000
VERSION     DAZFLD010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4508
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.280"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..620
                     /gene="hprR"
                     /locus_tag="ACHKBL_004482"
     CDS             <1..620
                     /gene="hprR"
                     /locus_tag="ACHKBL_004482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310734.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator transcription factor HprR"
                     /protein_id="HFO5821237.1"
                     /translation="GLSEAGYVIDAVSDGRDGLYLALKDDYALIILDIMLPGMDGWQI
                     LQTLRTAKQTPVICLTARDSVDDRVRGLDSGANDYLVKPFSFSELLARVRAQLRQHHA
                     LNSTLEISGLRMDSVSQSVSRNNISITLTRKEFQLLWLLASRAGEIIPRTVIASEIWG
                     INFDSDTNTVDVAIRRLRAKVDDPFPEKLIATIRGMGYSFVAVKK"
     gene            620..1978
                     /gene="hprS"
                     /locus_tag="ACHKBL_004483"
     CDS             620..1978
                     /gene="hprS"
                     /locus_tag="ACHKBL_004483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HprS"
                     /protein_id="HFO5821238.1"
                     /translation="MKKLSITVRLTLLFILVLSVAGAGIVWTLYNGLASELKWRDDTT
                     LINRTAQIKQLLIDGVNPDTLPVYFNRMMDVSQDILIIHGHGINKIVNRTNVSDGMLN
                     NIPASETISAAGIYRSIINDTEIDALRINIDEVSPSLTVTVAKLASARHNMLEQYKIN
                     SIIICIVAIVLCSVLSPLLIRTGLREIKKLSGVTEALNYNDNREPVEVSALPRELKPL
                     GQALNKMHHALVKDFERLSQFADDLAHELRTPINALLGQNQVTLSQTRSIAEYQKTIA
                     GNIEELENISRLTENILFLARADKNNVLVKLDSLSLNKEVENLLDYLEYLSDEKEICL
                     KVECNQQIFADKILLQRMLSNLIVNAIRYSPEKSRIQITSFLDTNGSLNIDIASPGTK
                     IHEPEKLFRRFWRGDNSRHSVGQGLGLSLVKAIAELHGGSATYHYLNKHNVFRITLPQ
                     RN"
     gene            complement(2085..2936)
                     /gene="hchA"
                     /locus_tag="ACHKBL_004484"
     CDS             complement(2085..2936)
                     /gene="hchA"
                     /locus_tag="ACHKBL_004484"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036524 - protein deglycase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase III HchA"
                     /protein_id="HFO5821239.1"
                     /translation="MTVQTSKNPQVDIAEDNAFFPSEYSLSQYTSPVSDLDGVDYPKP
                     YRGKHKILVIAADERYLPTDNGKLFSTGNHPIETLLPLYHLHAAGFEFEVATISGLMT
                     KFEYWAMPHKDEKVMPFFEQHKSLFRNPKKLADVVASLNADSEYAAIFVPGGHGALIG
                     LPESQDVAAALQWAIKNDRFVISLCHGPAAFLALRHGDNPLNGYSICAFPDAADKQTP
                     EIGYMPGHLTWYFGEELKKMGMNIINDDITGRVHKDRKVLTGDSPFAANALGKLAAQE
                     MLAAYAG"
     gene            complement(3528..>4508)
                     /locus_tag="ACHKBL_004485"
     CDS             complement(3528..>4508)
                     /locus_tag="ACHKBL_004485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HFO5821240.1"
                     /translation="VDFYGKMVGERIWSNTDDNNSENEDTSYARFGVKGETQITSELT
                     GFGQFEYNLDASKPEGSNQEKTRLTFAGLKYNELGSFDYGRNYGVAYDAAAYTDMLVE
                     WGGDSWASADNFMNGRTNGVATYRNSDFFGLVDGLNFAVQYQGKNSNRDVTKQNGDGY
                     ALSVDYNIEGFGFVGAYSKSDRTNEQAGDGYGDNAEVWSLAAKYDANNIYAAMMYGET
                     RNMTVLANDHFANKTQNFEAVVQYQFDFGLRPSLGYVYSKGKDLYARDGHKGVDADRV
                     NYIEVGTWYYFNKNMNVYTAYKFNLLDKDDAAITDAATDDQFAVGIVYQF"
BASE COUNT         1261 a          906 c         1057 g         1284 t
ORIGIN      
        1 aggggctttc cgaagcgggt tatgtcattg atgccgtttc tgatggcaga gatgggcttt
       61 atcttgcgct gaaggatgat tatgcattga tcattctgga tattatgctt ccgggtatgg
      121 atggctggca gatcttacaa acgttaagaa cggcaaagca aactcctgtt atttgcctta
      181 cagcaaggga ttctgtcgat gacagagtca gagggctgga cagtggagca aatgattatc
      241 tggtaaaacc tttttcattt tctgagttgc tggcaagggt tcgggcacaa ttaaggcaac
      301 atcacgcttt gaattcaaca ttagaaatca gtggcttaag aatggactct gttagtcaaa
      361 gtgtgagcag gaacaatatc agtattacac tgacgcgcaa ggagtttcag ttactttggc
      421 tactggcctc cagagctggc gaaattatac ccagaacggt tattgcgagt gaaatttggg
      481 gaatcaactt tgatagtgat accaatacgg tggatgtcgc cattcgcagg ctccgcgcaa
      541 aagttgatga tccttttcct gaaaagctca ttgccacaat ccgtgggatg ggatattcat
      601 tcgtagcggt aaaaaaataa tgaaaaaatt atctataacc gtccgtttaa ccttgctttt
      661 tatattggtg ctgtctgttg ctggcgctgg aattgtatgg actctctata atggcctggc
      721 aagtgagttg aaatggcgcg atgatacaac actcattaac cggacagcgc agatcaagca
      781 gttgttaatt gatggggtaa atccagatac gttacctgtc tattttaacc ggatgatgga
      841 tgttagtcag gatatcttga tcattcatgg tcatggcatc aataaaattg ttaaccggac
      901 aaatgtcagt gatggcatgt taaataacat acctgctagt gagacaatca gcgcagctgg
      961 catttacaga agcattatta atgacacaga gatagatgct ttacgaatta atattgatga
     1021 agtttcgcca tcattaacgg ttactgtggc taaattggct tcagccagac ataacatgct
     1081 tgaacagtat aaaattaata gcattataat ttgcattgtc gccattgtac tttgctcagt
     1141 attaagtccg ctgttaatca gaacggggtt acgagagatc aaaaagttga gtggtgtaac
     1201 ggaagcgctg aattataacg ataaccggga gcctgttgag gttagcgcat taccgagaga
     1261 actaaaacct cttgggcagg cgttgaataa aatgcatcat gctttagtca aagattttga
     1321 gcgtctaagt cagtttgctg acgatctcgc tcatgaactt agaacaccaa ttaatgcatt
     1381 actgggtcag aatcaggtta cgctcagtca aaccagaagt atcgctgaat atcaaaaaac
     1441 aattgccgga aacattgaag agctggagaa tatttcgcgg ttaacagaga acatactgtt
     1501 tcttgccagg gcagataaaa acaatgtttt ggtgaaactg gattcgcttt ctctcaataa
     1561 ggaagtcgaa aatttgttgg actatcttga atacctttca gacgagaaag agatttgcct
     1621 taaggtcgag tgcaatcagc aaatctttgc ggataaaatt ttactgcaac gaatgttatc
     1681 gaatcttatt gttaacgcta ttcgatattc accagaaaaa tcgcgcattc agataaccag
     1741 ttttcttgat accaacggct ctcttaatat tgatatcgcc agtcctggaa cgaaaattca
     1801 tgagcctgaa aaactcttcc gtagattttg gcggggagat aattcgcgtc attccgtagg
     1861 tcagggactt ggtctttctt tagtcaaagc gattgccgaa ttgcatgggg gaagtgctac
     1921 gtatcactat ctcaataagc ataacgtgtt ccgaattacg ttaccgcaaa gaaattaata
     1981 tagggcttca gtacgccaaa attaaccgtg aaaaaacgca gtcacgtcgt gagtattaac
     2041 gtggctgcga ttgataatgc gcttacatta caatgtaaca gggattaacc cgcgtaagct
     2101 gccagcattt cctgcgccgc cagtttaccc aacgcattcg ctgcgaaagg actgtcgccg
     2161 gtgagaactt tacggtcctt atgtactcgc ccggtgatgt cgtcattaat gatgttcatg
     2221 cccattttct tcagctcttc gccgaagtac caggtgagat gacccggcat atagcctatc
     2281 tctggcgttt gtttgtctgc ggcgtctggg aatgcgcaaa tggaataacc attcagtggg
     2341 ttatcgccgt ggcgaagcgc cagaaaagcc gccgggccgt ggcaaaggga gataacaaaa
     2401 cggtcatttt tgattgccca ctgtaaagcg gcagctacgt cctggctttc aggtaaacca
     2461 ataagtgcgc catgaccacc aggaacaaag attgctgcat attcgctatc agcgttgagg
     2521 cttgcgacaa catccgcgag tttcttcgga ttgcggaaca acgatttatg ctgctcaaag
     2581 aatggcatta ctttttcatc tttgtgtggc atagcccagt attcaaactt ggtcatcaga
     2641 ccggaaatgg tcgccacttc gaattcgaaa cctgcagcat ggagatgata caacggcagc
     2701 aacgtttcaa tcggatggtt accggtcgag aatagtttcc cgttatcggt cggcaaataa
     2761 cgttcgtccg cggcgatcac cagaatttta tgtttaccgc gatacggttt tggatagtcc
     2821 acgccatcaa gatcagagac gggactggta tattggctaa gcgaatattc tgaagggaag
     2881 aatgcattat cttcagcaat atcgacctgc gggtttttac ttgtttgaac agtcatagtg
     2941 tattccttat tgttgcttag ttagggtagt cactatattt gcgactgagc tggtcgctat
     3001 ttttaaccag gctgaaaaat tgagtcaata tatcgaaagc ggtagttggc gggcataagt
     3061 atgtaatgat gatgctgcac tggtgttgaa tattattcac gccaaatttt ccttattgag
     3121 ctacgtcgtc gcaagtggcc tcaatgattt tagaccatta tcagaggtcc ggggcggcag
     3181 ggagagattt agtgctgtgg aatattgtgg tgctgatgaa tatttatatc gcggattggt
     3241 agagaaaaag cttttttcat gcactatttt tgcaaatatt tctatatgga cggcggtttt
     3301 gttgcggatt aattcataaa gaaatgcatt tggatgttgg ccggaagaga cgctggtaga
     3361 ttttattgag ccaggagggg tggggtaagg tgctgtatcg atatgctttg gcaattgcgt
     3421 gtgccgcaga tggagatgaa atgccaataa aaaagcctga gttcgatgag ggacgaactc
     3481 aggctggtgt taagttttac agtaacacgc cgcggcgcgt tggctgatta gaactggtag
     3541 acgataccaa ctgcaaactg gtcatcagtt gcggcatcgg taatcgctgc atcgtcttta
     3601 tccagcaggt taaatttgta tgctgtgtag acgttcatgt tcttattgaa gtagtaccag
     3661 gtaccaactt cgatataatt tacgcggtca gcatcaacac ctttatgtcc atcacgagca
     3721 taaagatctt tgcctttgga atatacgtag cctaaagacg gacgtaaacc gaagtcgaac
     3781 tgatactgta caacagcttc aaagttttgg gttttatttg caaaatgatc attagccaaa
     3841 acggtcatgt tgcgggtttc accgtacatc attgctgcat agatattatt tgcatcatac
     3901 ttggctgcta atgaccacac ttcagcgtta tcaccgtagc cgtcaccagc ttgttcatta
     3961 gtacgatcag atttgctata tgcacctaca aaaccaaaac cttcgatgtt gtagtctaca
     4021 gacaacgcat agccatcacc gttttgtttc gtaacgtcac gattgctgtt cttaccttga
     4081 tattgcacag caaaattcag accatcaacc agaccaaaga aatcagagtt acggtaggtt
     4141 gcaacaccgt tggtacgacc gttcatgaag ttgtcagcgg acgcccagga atcaccaccc
     4201 cattcaacta acatatcggt ataagctgcg gcatcataag caacaccgta gttacgacca
     4261 tagtcgaaag atcctaactc gttatatttc aaacctgcga aggttaaacg ggttttttcc
     4321 tgattagagc cttcgggctt gctggcgtcg aggttgtatt caaactgacc aaaaccagtc
     4381 agttcgctgg tgatttgggt ttcaccttta acaccgaaac gagcatagga ggtatcttcg
     4441 ttttcgctgt tattatcatc agtatttgac cagatgcgtt cgccaaccat cttgccgtag
     4501 aaatcaac
//
LOCUS       DAZFLD010000279         4505 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.281, whole genome shotgun sequence.
ACCESSION   DAZFLD010000279 DAZFLD010000000
VERSION     DAZFLD010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4505
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.281"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            226..1383
                     /gene="acrE"
                     /locus_tag="ACHKBL_004486"
     CDS             226..1383
                     /gene="acrE"
                     /locus_tag="ACHKBL_004486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrE"
                     /protein_id="HFO5821241.1"
                     /translation="MTKHARFFLLPSFILISAALIAGCNDKGEEKAHVGEPQVTVHIV
                     KTAPLEVKTELPGRTNAYRIAEVRPQVSGIVLNRNFTEGSDVQAGQSLYQIDPATYQA
                     SYDSAKGELAKSEAAAAIAHLTVKRYVPLVGTKYISQQEYDQAIADARQADAAVIAAK
                     ATVESARINLAYTKVTAPISGRIGKSTVTEGALVTNGQTTELATVQQLDPIYVDVTQS
                     SNDFMRLKQSVEQGNLHKENATSNVELVMENGQTYPLKGTLQFSDVTVDESTGSITLR
                     AVFPNPQHTLLPGMFVRARIDEGVQPDAILIPQQGVSRTPRGDATVLIVNNKSQVEAR
                     PVVASQAIGDKWLISEGLKSGDQVIVSGLQKARPGEQVKATTDTPADTASK"
     gene            1395..4499
                     /gene="acrF"
                     /locus_tag="ACHKBL_004487"
     CDS             1395..4499
                     /gene="acrF"
                     /locus_tag="ACHKBL_004487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit AcrF"
                     /protein_id="HFO5821242.1"
                     /translation="MANFFIRRPIFAWVLAIILMMAGALAILQLPVAQYPTIAPPAVS
                     VSANYPGADAQTVQDTVTQVIEQNMNGIDNLMYMSSTSDSAGSVTITLTFQSGTDPDI
                     AQVQVQNKLQLATPLLPQEVQQQGISVEKSSSSYLMVAGFVSDNPGTTQDDISDYVAS
                     NVKDTLSRLNGVGDVQLFGAQYAMRIWLDADLLNKYKLTPVDVINQLKVQNDQIAAGQ
                     LGGTPALPGQQLNASIIAQTRLKNPEEFGKVTLRVNSDGSVVRLKDVARVELGGENYN
                     VIARINGKPAAGLGIKLATGANALDTAKAIKAKLAELQPFFPQGMKVLYPYDTTPFVQ
                     LSIHEVVKTLFEAIMLVFLVMYLFLQNMRATLIPTIAVPVVLLGTFAILAAFGYSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMMEDKLPPKEATEKSMSQIQGALVGIAMVLS
                     AVFIPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATLLKPVSAEHHENK
                     GGFFGWFNTTFDHSVNHYTNSVGKILGSTGRYLLIYALIVAGMVVLFLRLPSSFLPEE
                     DQGVFLTMIQLPAGATQERTQKVLDQVTDYYLKNEKANVESVFTVNGFSFSGQAQNAG
                     MAFVSLKPWEERSGDENSAEAVIHRAKMELGKIRDGFVIPFNMPAIVELGTATGFDFE
                     LIDQAGLGHDALTQARNQLLGMAAQHPASLVSVRPNGLEDTAQFKLEVDQEKAQALGV
                     SLSDINQTISTALGGTYVNDFIDRGRVKKVYVQADAKFRMLPEDVDKLYVRSANGEMV
                     PFSAFTTAHWVYGSPRLERYNGLPSMEIQGEAAPGTSSGDAMALMENLASQLPAGIGY
                     DWTGMSYQERLSGNQAPALVAISFVVVFLCLAALYESWSIPVSVMLVVPLGIVGVLLA
                     ATLFNQKNDVYFMVGLLTTIGLSAKNAILIVEFAKDLMEKEGKGVVEATLMAVRMRLR
                     PILMTSLAFILGVLPLAISNGAGSGAQNAVGIGVMGGMVSATLLAIFFVPVFFVVIRR
                     CFKG"
BASE COUNT         1097 a         1091 c         1185 g         1132 t
ORIGIN      
        1 aatttacgag gatttgattc tacctcttta aacccgcgtc aaaataaaac agtagaatat
       61 taatcttttt ttgtgtttat gtgccttgag acgccagtat tcataactat tccttacatc
      121 gacgaatgat aatttgtagg atagcgaact gtatttttct ttctgcgagt taacgcgttg
      181 cctttttggg taaataacgc gcttttggtt ttttgaggaa tagtaatgac gaaacatgcc
      241 aggtttttcc tcctgccctc ctttattctg atctccgcgg ctttaatcgc cggttgtaac
      301 gataagggag aagagaaagc tcacgtcggt gaaccgcagg ttaccgttca tattgtgaaa
      361 acggccccgt tagaagttaa gactgaatta ccaggccgca ccaatgctta ccgtatagcc
      421 gaagttcgcc cacaggttag cgggatcgta ctgaatcgca atttcactga aggcagcgat
      481 gtgcaagcag gccagtccct gtaccagatc gatcccgcga cctatcaggc aagttatgac
      541 agcgcgaaag gcgaactagc gaaaagtgaa gccgccgccg ccatcgcgca tttgacggta
      601 aaacgttacg ttccgctcgt gggtacgaaa tacatcagtc agcaggagta cgaccaggcc
      661 attgctgatg ctcgccaggc cgatgccgcc gtgattgccg caaaagctac agttgaaagc
      721 gctcgcatca atctcgctta taccaaagtg actgcgccta ttagcggacg tatcggcaaa
      781 tcgactgtga ccgaaggcgc tcttgtcact aatgggcaaa cgactgaact ggcgactgtc
      841 cagcagctcg atcctatcta cgttgatgtg acccaatcca gcaacgattt tatgaggctg
      901 aagcaatccg tagagcaagg aaatttgcat aaggaaaacg ccaccagcaa cgtagagttg
      961 gtcatggaga acggtcaaac ctatcccctg aaaggtacgc tgcaattctc cgatgtgacc
     1021 gttgatgaaa gcaccggctc cataacccta cgtgctgtct tccctaaccc gcaacatacg
     1081 cttttgccgg gtatgtttgt gcgcgcacgg attgatgaag gcgtccaacc tgacgccatt
     1141 cttatcccgc aacaaggcgt tagccgcaca ccacgtggtg atgcaaccgt gctgattgtt
     1201 aacaataaaa gtcaggttga agcgcgccct gtcgttgcca gccaggcgat tggcgataaa
     1261 tggttgatta gcgaagggct gaaatctggc gatcaagtca ttgtcagcgg cctacaaaaa
     1321 gcgcgtccgg gagagcaggt taaagccact accgataccc ccgcagatac tgcatcgaag
     1381 taaggcaatc tgacatggca aactttttta ttcgacgacc gatatttgca tgggtgctgg
     1441 ccattattct gatgatggcg ggcgcactgg cgatcctaca attgcccgtc gctcagtatc
     1501 caaccattgc accgcctgcg gtttctgttt cagcaaacta tccgggcgct gatgcgcaga
     1561 ccgtgcagga tacggtgacg caggttatcg aacagaatat gaacggtatc gataacctga
     1621 tgtatatgtc ctccaccagc gattccgccg gtagcgtgac aattaccctt accttccagt
     1681 ccgggaccga tcctgatatc gcgcaagtgc aggtgcagaa caaactccag ctcgccacgc
     1741 cgttgctgcc gcaggaagta cagcagcagg ggatcagtgt tgaaaagtcc agtagcagct
     1801 atttgatggt ggcgggcttt gtctctgata acccaggcac cacacaggac gatatctcgg
     1861 actatgtggc ctctaacgtt aaagatacgc ttagccgtct gaatggcgtc ggtgacgtac
     1921 agcttttcgg cgcacagtat gcgatgcgta tctggctgga cgccgatctg ctaaacaaat
     1981 ataaactgac accggttgat gtgattaacc agttgaaggt acagaacgat cagatcgctg
     2041 ccggacagtt gggcggaacg ccagcgttac cagggcaaca gttgaacgcc tcgattattg
     2101 ctcagacgcg gttaaaaaat ccggaagaat tcggcaaagt gaccctgcgc gtaaacagtg
     2161 acggctcggt ggtacgcctg aaagatgtcg cacgggttga acttggcggt gaaaactaca
     2221 acgttatcgc tcgtatcaac ggaaaaccgg cggcgggcct ggggattaag ctggcaaccg
     2281 gcgcaaatgc tctcgatacc gcgaaagcca ttaaggcaaa actggcggaa ttacagccat
     2341 tcttcccgca gggaatgaag gttctctacc cttatgacac cacaccattc gtccagcttt
     2401 ctattcacga agtggtaaaa acgctgttcg aagccattat gctggtgttc ctggtgatgt
     2461 atctgttctt gcagaatatg cgagcaacgc tgatccccac cattgcggta cccgtggtgt
     2521 tgttagggac gtttgccatc ctcgccgctt ttggttactc catcaacaca ctaacgatgt
     2581 tcgggatggt gcttgccatc gggctgctcg tcgatgatgc gatagtggtg gtggagaacg
     2641 tcgagcgcgt gatgatggag gataagctcc cgccaaaaga agcgacggaa aaatcaatgt
     2701 cgcaaatcca gggcgcactg gtgggtatcg caatggtgct gtcagcggta tttattccga
     2761 tggcattctt cggcggttct actggggcaa tttatcgtca gttctctatc accatcgttt
     2821 cggcaatggc gctttctgtt ctggtggcat tgattcttac ccctgcgtta tgtgcaacgc
     2881 tgcttaaacc cgtctctgct gagcatcacg aaaataaggg cggtttcttc ggctggttta
     2941 ataccacctt cgatcatagc gttaaccact acaccaacag cgtcggcaaa atcctcggtt
     3001 ccacaggacg atatttactg atctatgcac taattgttgc aggaatggtg gtgttgtttt
     3061 tacgtcttcc gtcttccttc ttacctgaag aggatcaggg tgtctttctg actatgattc
     3121 agttacccgc tggcgcgacg caagagcgga cgcaaaaagt actggatcaa gtgacggatt
     3181 actatctgaa gaacgagaaa gcgaacgttg aaagtgtctt tacagttaac ggctttagct
     3241 tcagcggcca ggcacaaaac gccggtatgg ctttcgtcag tctgaaaccg tgggaagagc
     3301 gtagtggtga cgaaaacagt gcggaagcgg taatccatcg tgccaaaatg gaattgggca
     3361 agatccgcga cggttttgtc attccattca atatgccagc cattgttgaa ctgggcacgg
     3421 caacgggttt cgactttgag ttaattgatc aggctgggct gggtcatgat gccctgaccc
     3481 aggcccgtaa ccagttgctt ggcatggcgg cgcaacatcc tgccagctta gtcagcgtgc
     3541 gtcctaatgg tctggaagac accgcgcagt ttaaactgga agttgaccaa gaaaaggcgc
     3601 aggcattagg tgtttcactt tctgacatca atcagaccat ttcaacagcg ttgggtggga
     3661 cttacgttaa cgacttcatt gaccgtggtc gcgtaaaaaa ggtgtatgtt caggcggatg
     3721 ccaaatttcg tatgctgcca gaagatgtcg ataaacttta tgtccgtagc gccaatggcg
     3781 aaatggtgcc attctctgcc tttaccactg cacattgggt gtatggttct ccgcgactgg
     3841 agcgctacaa cggtctgccg tcaatggaga ttcaggggga agccgcgcca ggaaccagtt
     3901 ccggcgatgc catggcgttg atggaaaacc ttgcatcaca attaccagct ggtattggtt
     3961 atgactggac gggtatgtca tatcaggaac gcttatcggg aaaccaggct cccgctctgg
     4021 tagcaatttc ctttgtggtt gttttcctgt gccttgctgc actctatgaa agctggtcaa
     4081 ttcctgtctc ggttatgttg gtcgtgccgt tagggattgt cggcgtgcta ctggcggcga
     4141 cactctttaa tcaaaaaaat gacgtctact ttatggtggg cttgctaacg acaattggct
     4201 tgtcggctaa aaacgctata ttgattgttg agttcgctaa agatctcatg gagaaagagg
     4261 gtaaaggtgt tgttgaagcg acactaatgg cagtacgtat gcgtctgcgt cctatcctga
     4321 tgacctctct cgcctttatt ctcggcgtat taccgctagc tatcagtaac ggtgccggca
     4381 gtggcgcgca gaacgcagtg ggtatcgggg taatgggcgg gatggtttct gcaacgttgc
     4441 tggcaatctt cttcgtgccg gtgttctttg tggtgatccg ccgttgcttt aaaggataaa
     4501 tcaga
//
LOCUS       DAZFLD010000280         4487 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.282, whole genome shotgun sequence.
ACCESSION   DAZFLD010000280 DAZFLD010000000
VERSION     DAZFLD010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4487
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.282"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            39..833
                     /gene="hyxR"
                     /locus_tag="ACHKBL_004488"
     CDS             39..833
                     /gene="hyxR"
                     /locus_tag="ACHKBL_004488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001304859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR family transcriptional regulator HyxR"
                     /protein_id="HFO5821243.1"
                     /translation="MKKPLVIISACQFTRLALESLIPTDRYIVRVYSNVTTEVEFVLK
                     TSCGYLLADIPSLPSGELVLLACLVRLRSDAGQWQVCLTGDSDWFDNAPAASLYNGFP
                     RIGTMLTASRYQNQIIRWLLRPLPGDMSDFWLLTARELSVLKILMQGMSFSEIARYEQ
                     RSSKTLHAIATRALMKLGLGTLSDFRLLYTGCGDSRVKRNIRLHAKYIGQHSAHRLTQ
                     YVQNMVSGILYPEKVAVGESINSDAISGKVLSGQRKNMSKKRPGLL"
     gene            987..1748
                     /gene="hyxA"
                     /locus_tag="ACHKBL_004489"
     CDS             987..1748
                     /gene="hyxA"
                     /locus_tag="ACHKBL_004489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein HyxA"
                     /protein_id="HFO5821244.1"
                     /translation="MRFVLFCPSGIVPAQFAALSTGSVGNVTCIRTEEELRNKLRHRP
                     QSVVISAGRPAECAEMWFRFYRDHSFVVVLCVAPFFLPPDVSISGVLKNLRLLKPGMS
                     VERVISIANASGGFSGLKHAEILPVMDSYSVFMKEVNNRTKTIVMSERFPEKQKKVLS
                     LLLAGHSWEYSAQFLKTGIRQIWLAEQSLKKRWGIPDSMSLREALLLSSNNFHGDGNA
                     LETTNAMTLRENGNTNRYSVVANAGTQALSLHKYK"
     gene            1790..4372
                     /locus_tag="ACHKBL_004490"
     CDS             1790..4372
                     /locus_tag="ACHKBL_004490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFO5821245.1"
                     /translation="MKNSKAFYRSALATAIVMALSAPAFAADKAVSTDSVTLNKDKTT
                     LDQDVVINGDNKITAVTIETSDSDKDLNVTFGGHDITAASTVNQGFVEGVKVSGDKNV
                     VINATGSTITAQGEGTYVRSAMVISSTGDVVVNGGNFVAKNEKGSATGISLEGAQGNN
                     VTLNGTTINAQGNKSSSNASTAIFAQKGSVLNGFNGDATDNITLAGSNIINGRIETIL
                     IAQENKGTHTVNLKIKDGSVIGAANNKQTIYASASAQGKGSATQNLNLSVADSTIYSD
                     VLALSESENSAATTTNVNMNVARSYWEGNAYAYAYAYAYAGVYADDDKASSNLNINLS
                     DGSVWKGNVAGSTTTGSTGTTENQSATVSLSDSTWKGNVGDGLDGGARVTLKNSSWTG
                     DVGNAKTAVFLTDGSVWNGAVSINAPVDRASVPMGSVQPNKSSVSLQNGSVWNVTGAS
                     TVDALAVKDSTVNITKATVNTGTFASQNGTLIVDASSENTLDISGKASGDLRVYSAGS
                     LDLINEQTAFISTGKDSTLKATGTTEGGLYQYDLTQGADGNFYFVKNTHKASNASSVI
                     QAMAAAPANVANLQADTLSARQDAVRLSENDEGGVWIQYFGGKQKHTTAGNASYDLDV
                     NGVMLGGDTRFMTEDGSWLAGVAMSSAKGDMTTMQSKGDTEGYSFHAYLSRQYNNGIF
                     IDTAAQFGHYSNTADVRLMNGGGTIKADFNTNGFGAMVKGGYTWKDGNGLFIQPYAKL
                     SALTLEGVDYQLNGVDVHSDSYNSVLGEAGTRVGYDFAVGNSTVKPYLNLAALNEFSD
                     GNKVRLGDESVNASIDGAAFRVGAGVQADITKNMGAYASLDYTKGDDIENPLQGVVGI
                     NVTW"
BASE COUNT         1212 a          979 c         1147 g         1149 t
ORIGIN      
        1 aatgtcaggt tttgtagcaa tggcgtggac gagtctgaat gaagaagcct ctggttatta
       61 tcagtgcctg tcagttcacc aggctggcac tggagtctct catcccaacg gacaggtata
      121 tcgtgcgggt gtacagcaac gtcactacag aggttgaatt cgttttaaag acctcctgtg
      181 gctacctgtt agctgatatc ccatcgcttc cttcgggaga gttggttctt ctggcttgtc
      241 ttgtccgctt gcggagtgat gccgggcaat ggcaggtctg cctgaccggt gactccgact
      301 ggttcgataa tgctcctgct gcaagtctgt ataacggctt cccacgcatc ggcaccatgc
      361 tgacagcgag tcgttatcag aatcaaatta tccgctggtt actacgtcct ttgcccggcg
      421 atatgagcga tttctggctc ctgactgcac gggagttgag cgtgcttaag attctgatgc
      481 agggtatgag tttcagtgag attgcccgtt atgaacaacg cagttcaaag accctgcatg
      541 cgattgccac acgggcttta atgaaacttg gactgggaac actgagcgat ttcaggctgc
      601 tctatacggg atgcggtgac agcagagtga aacggaatat acggctacat gcaaaatata
      661 tcggacaaca ttccgctcat cgactcactc agtatgtgca gaacatggta tccggaattc
      721 tttatccaga gaaagttgcc gttggtgaat caataaattc tgacgccata tccggaaaag
      781 tactttccgg acaaagaaaa aatatgtcta aaaaaagacc aggattgtta tgacacagca
      841 gcgttgttac taccccccac tgcggcgcat acggactcgc tacagtgtag gagccaaaat
      901 atttctactg ctctgggctg caggattggg aggcacgctg tacagcctgt acagcgtgct
      961 ggcgtactga catctgtgag ggaaggatgc gtttcgttct tttctgtccc tcaggaattg
     1021 taccagcgca gttcgcagct ctatcaaccg gttctgtcgg caatgttacc tgcatcagaa
     1081 cagaagagga gttacgcaat aaattgcggc acaggccaca gagtgtcgtg atatcggcag
     1141 ggcgacctgc cgaatgcgct gaaatgtggt tccgatttta tcgggatcac tcattcgtgg
     1201 tggtgttgtg cgtcgcaccc ttttttctgc caccagatgt gagtatttct ggcgttctca
     1261 aaaacctgag gttactgaaa ccaggaatgt cagtagagcg tgtcatcagt attgcaaatg
     1321 cctctggtgg gttttcaggg ctgaaacatg cagaaatttt gccagttatg gacagttact
     1381 ctgtctttat gaaagaggtg aataaccgca caaagacaat cgtcatgtca gaacgcttcc
     1441 cggaaaagca aaagaaggtg ttgtcgttgc ttcttgccgg acacagctgg gaatacagtg
     1501 cgcagtttct caaaacgggg ataagacaga tatggcttgc cgagcagtcg ctaaaaaagc
     1561 gttggggaat accggacagc atgtcattgc gggaagccct gctgctgagc agcaataatt
     1621 ttcacggtga tggaaatgca ttggaaacaa cgaatgccat gacattgcgt gaaaacggaa
     1681 ataccaacag gtattccgtg gtggcaaatg cagggacgca ggctttatct ctgcataaat
     1741 ataaataagt gggaaattga aattagttac cagaatggag aatttcttca tgaaaaacag
     1801 taaggcattt taccgcagcg cattagcaac agctattgtt atggctcttt ctgcaccagc
     1861 attcgctgct gataaagcgg tatcaactga ttcggttacg ctgaataaag ataagacaac
     1921 tctggatcaa gatgttgtta ttaacggtga taacaagatt acagccgtaa caattgaaac
     1981 gtcagattca gataaagacc ttaatgttac ttttggcggt cacgatatta ccgccgcatc
     2041 aacggtaaac caaggtttcg ttgaaggtgt aaaagttagc ggtgacaaaa atgttgtgat
     2101 taatgctaca ggctccacca tcacagctca aggtgaaggc acctatgtcc ggtctgcaat
     2161 ggtcatttct tcaactggcg atgttgttgt taatggcggt aatttcgttg caaaaaatga
     2221 aaaaggtagt gcgacaggga tatctctgga aggggcccag ggaaataatg tcacgcttaa
     2281 tggcacaacc ataaatgctc aaggtaataa gagttccagc aacgcctcca cggcaatttt
     2341 tgctcaaaag ggtagcgtat tgaatggttt taacggtgat gcaacagaca acattaccct
     2401 tgctggctca aatattatta atggtcggat tgaaacaata cttattgctc aggagaataa
     2461 gggaactcat acagtcaacc tgaagattaa ggatggctca gtaattgggg cggctaataa
     2521 taaacaaaca atttatgcct cagcttcggc acaaggaaaa ggttcagcaa cgcaaaattt
     2581 aaatttgtct gttgctgatt caaccatcta ctctgatgtc ctggcccttt ctgaaagcga
     2641 gaattcagcc gctaccacaa caaatgtaaa tatgaacgtt gcccgctctt actgggaagg
     2701 taatgcttat gcttatgctt atgcttatgc ttatgctggt gtttatgctg atgacgataa
     2761 agcgagtagt aatctgaata taaatctttc cgatggctca gtctggaaag gaaatgttgc
     2821 gggtagtacg actactggca gcaccggtac tacagaaaac cagtcagcaa ccgtaagtct
     2881 gtctgattcc acctggaaag gtaacgtggg tgacggtctg gatggtggtg cgcgagtcac
     2941 gctgaaaaac tccagctgga ctggtgatgt cggcaacgcc aaaactgctg tttttctgac
     3001 ggatggttct gtgtggaatg gtgctgtttc cattaacgcg ccagtggacc gcgcatcagt
     3061 accaatgggc tcggttcaac caaataaaag cagtgtatct ctgcaaaacg ggtctgtctg
     3121 gaatgttacg ggagcctcaa ctgttgatgc tctggcagta aaagacagta cggttaatat
     3181 cacgaaggct acagtcaata ctggcacgtt tgcttctcaa aacggcactc tgattgttga
     3241 tgcctcttct gaaaacaccc tggatatcag cggtaaagcg agcggtgact tgcgtgttta
     3301 cagtgcgggt tcattggatc ttatcaatga acaaacggca tttatttcta ccggcaaaga
     3361 cagcactcta aaagccacag gcacaacgga aggtggtctg tatcaatatg acctgacaca
     3421 gggagctgat ggtaactttt atttcgtaaa aaacacgcat aaagcatcca acgccagctc
     3481 cgtgattcag gcaatggcag ctgctccggc taacgtcgct aatctgcagg ccgacacgct
     3541 ctccgcccgt caggatgctg tccgtctgag cgaaaatgac gagggtggcg tatggattca
     3601 gtactttggc ggtaaacaga aacataccac cgcaggaaat gcatcctatg acctggatgt
     3661 aaacggtgta atgctgggtg gtgatacccg cttcatgact gaagatggta gctggctggc
     3721 cggtgtggcg atgtcttctg cgaaaggtga catgaccacc atgcagagca aaggtgacac
     3781 tgaaggttac agcttccacg cttacctgag ccgccagtat aacaacggta tcttcattga
     3841 tactgctgca cagtttggtc attacagcaa cacggcagat gttcgcctga tgaatggtgg
     3901 cggtaccatc aaagctgact ttaacaccaa tggttttggt gcgatggtta aaggcggtta
     3961 cacatggaaa gacggtaatg gcctgtttat tcagccatat gccaaactgt ctgctctgac
     4021 gctggaaggt gtggattatc aactcaacgg cgtggacgtt cattctgaca gctataactc
     4081 tgtgctgggt gaggccggta cgcgcgtggg ttatgacttc gctgtgggca actcgaccgt
     4141 taaaccttat ctgaatctgg ccgcactgaa cgaattctct gatggcaaca aagtccgtct
     4201 gggtgatgag tctgtcaatg ccagcattga cggtgcagca ttccgcgtgg gtgcaggtgt
     4261 acaggctgat atcaccaaaa acatgggagc atatgcaagc cttgactaca ccaaaggtga
     4321 cgacattgag aatccgctac agggtgtagt tggtatcaat gtgacctggt aagaataaag
     4381 cagtcggttc ccctgtcgac tgttataccc cctgcaggca gcagtatgct gcctgcaggc
     4441 tataaatatc cagtattcat gatgctggat atttgctgat atttaac
//
LOCUS       DAZFLD010000281         4432 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.283, whole genome shotgun sequence.
ACCESSION   DAZFLD010000281 DAZFLD010000000
VERSION     DAZFLD010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4432
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.283"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..610
                     /locus_tag="ACHKBL_004491"
     CDS             <1..610
                     /locus_tag="ACHKBL_004491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFO5821246.1"
                     /translation="LCLGIGYAVIRRTIFIVYPPILSNGMFNFIVMQTLFYLPFFILG
                     ALAFIFPHLKALFTTPSRGCTLAAALAFVAYLLNQRYGSGDAWMYETESVITMVLGLW
                     MVNVVFSFGHRLLNFQSARVTYFVNASLFIYLVHHPLTLFFGAYITPHITSNWLGFLC
                     GLIFVVGIAIILYEIHLRIPLLKFLFSGKPVVKRENDKAPAR"
     gene            complement(618..2099)
                     /gene="clsC"
                     /locus_tag="ACHKBL_004492"
     CDS             complement(618..2099)
                     /gene="clsC"
                     /locus_tag="ACHKBL_004492"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsC"
                     /protein_id="HFO5821247.1"
                     /translation="MMKKTPTSTKDSLLNKEMNDLPRLASAVLPLCSQHPGQCGIFPL
                     EKSLDAFAARYRLAEMAEHTLDVQYYIWQDDMSGRLLFSALLAAAKRGVRVRLLLDDN
                     NTPGLDDILRLLDSHPRIEVRLFNPFSFRLLRPLGYITDFSRLNRRMHNKSFTVDGVV
                     TLVGGRNIGDAYFGAGEEPLFSDLDVMAIGPVVEDVADDFARYWYCKSVSPLQQVLDV
                     PEGEMADRIELPASWHNDAMTHRYLRKMESSPFINHLVDGTLPLIWAKTRLLSDDPAK
                     GEGKAKRHSLLPQRLFDIMGSPSERIDIISSYFVPTRAGVAQLLRMVRKGVKIAILTN
                     SLAANDVAVVHAGYARWRKKLLRYGVELYELKPTREQSSTLHDRGITGNSGASLHAKT
                     FSIDGKTVFIGSFNFDPRSTLLNTEMGFVIESETLAQLIDKRFIQSQYDAAWQLRLDR
                     WGRINWVDRHAKKEIILKKEPATSFWKRVMVRLASILPVEWLL"
     gene            complement(2041..2574)
                     /gene="ymdB"
                     /locus_tag="ACHKBL_004493"
     CDS             complement(2041..2574)
                     /gene="ymdB"
                     /locus_tag="ACHKBL_004493"
                     /EC_number="3.1.1.106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HFO5821248.1"
                     /translation="MKTRIHVVQGDITKLAVDVIVNAANPSLMGGGGVDGAIHRAAGP
                     ALLDACLKVRQQQGDCPTGHAVITLAGDLPAKAVVHTVGPVWRGGEQNEDQLLQDAYL
                     NSLRLVAANSYTSVAFPAISTGVYGYPRAAAAEIAVKTVSEFITRHALPEQVYFVCYD
                     EENAHLYERLLTQQGDE"
     gene            complement(2669..2980)
                     /gene="ymdA"
                     /locus_tag="ACHKBL_004494"
     CDS             complement(2669..2980)
                     /gene="ymdA"
                     /locus_tag="ACHKBL_004494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmdA"
                     /protein_id="HFO5821249.1"
                     /translation="MFRPFLDSLMLGSMFFPFIAIAGSTAQGGVIHFYGQIVEPACDV
                     STQSSPVEMNCPQNGSVPGRTYSSKALMSGNVKNAQIASVKVQYLDKQKKLAVMNIEY
                     N"
     gene            complement(3101..3433)
                     /gene="csgC"
                     /locus_tag="ACHKBL_004495"
     CDS             complement(3101..3433)
                     /gene="csgC"
                     /locus_tag="ACHKBL_004495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly chaperone CsgC"
                     /protein_id="HFO5821250.1"
                     /translation="MNALLLLAALSSQITFNTTQQGDMYTIIPEVTLTQSCLCRVQIL
                     SLREGSSGQSQTKQEKTLSLPANQPIALTKLSLNISPDDRVKIVVTVSDGQSLHLSQQ
                     WPPSSEKS"
     gene            complement(3492..3947)
                     /gene="csgA"
                     /locus_tag="ACHKBL_004496"
     CDS             complement(3492..3947)
                     /gene="csgA"
                     /locus_tag="ACHKBL_004496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli major subunit CsgA"
                     /protein_id="HFO5821251.1"
                     /translation="MKLLKVAAIAAIVFSGSALAGVVPQYGGGGNHGGGGNNSGPNSE
                     LNIYQYGGGNSALALQADARNSDLTITQHGGGNGADVGQGSDDSSIDLTQRGFGNSAT
                     LDQWNGKDSTMTVKQFGGGNGAAVDQTASNSSVNVTQVGFGNNATAHQY"
     gene            complement(3988..4431)
                     /gene="csgB"
                     /locus_tag="ACHKBL_004497"
     CDS             complement(3988..4431)
                     /gene="csgB"
                     /locus_tag="ACHKBL_004497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli minor subunit CsgB"
                     /protein_id="HFO5821252.1"
                     /translation="MLFMMLTILGAPGIAAAAGYDLANSEYNFAVNELSKSSFNQAAI
                     IGQAGTNNSAQLRQGGSKLLAVVAQEGSSNRAKIDQTGDYNLAYIDQAGSANDASISQ
                     GAYGNTAMIIQKGSGNKANITQYGTQKTAIVVQRQSQMAIRVTQR"
BASE COUNT         1112 a         1117 c          999 g         1204 t
ORIGIN      
        1 tttgtgcctc ggcatcggtt atgcggtaat aagaagaacg atttttattg tgtatccgcc
       61 cattctgagt aatggcatgt tcaattttat tgtcatgcaa acgctatttt atttgccgtt
      121 ctttatcctc ggcgcactgg ctttcatttt ccctcatctt aaagccttgt ttaccacgcc
      181 gtctcgtggc tgtacccttg cagcagcatt ggcatttgtc gcttatttac tcaaccagcg
      241 ctatggcagt ggcgatgcct ggatgtacga aaccgagtcg gtgatcacca tggtcctcgg
      301 tctgtggatg gtgaatgtgg tcttctcctt tggccaccgt ttgcttaact tccagtcagc
      361 gcgggtgact tattttgtta acgcatcgct gtttatctat ctggttcacc acccgttaac
      421 gctgtttttc ggcgcataca ttacaccgca catcacctcc aactggcttg gttttctctg
      481 tggcctgata ttcgtagtag ggattgcgat aattctgtat gaaattcatt tacgcatccc
      541 gttactgaag tttttgttct ctggtaaacc ggttgtcaag cgtgagaacg ataaagcacc
      601 agcccgttaa gccacgctta taataaccat tccacgggca atatcgacgc cagtctgacc
      661 ataacccgct tccagaaact ggtggcgggt tcttttttga gaataatctc tttctttgca
      721 tgacgatcaa cccagttgat ccgtccccac ctgtccagac ggagctgcca ggccgcatca
      781 tactggctct gaataaagcg tttatcaatt aactgtgcca gcgtttcgct ctctatcacg
      841 aagcccattt cagtattgag caatgttgaa cgcggatcga aattgaaaga gccgataaac
      901 accgttttac catcgatgct aaaggtttta gcatgcaagc tggcaccgga attaccggtt
      961 atgccgcgat cgtgtaacgt actactttgt tcacgcgtcg gcttgagttc atataattcc
     1021 acgccatagc ggagcaattt tttgcgccag cgcgcgtatc cggcatggac gacagcaaca
     1081 tcgttagcgg caagagaatt ggttaggatc gcaatcttta ccccttttct caccatccgt
     1141 aagagttgcg ccacacctgc gcgtgtcggt acaaaatagg aagagataat atcgatgcgt
     1201 tcactgggtg agcccatgat atcgaacagg cgctgcggta acagtgaatg ccgttttgcc
     1261 ttgccttccc ctttcgccgg atcatcactt aataaacgtg ttttcgccca gataagcggc
     1321 aacgttccat caaccagatg atttataaac gggctggatt ccattttgcg taaataacga
     1381 tgcgtcatgg catcgttgtg ccaggatgct ggtaactcga tgcgatccgc catttcacct
     1441 tccgggacat ccagcacctg ctgtaggggt gaaaccgatt tgcaatacca gtagcgggcg
     1501 aaatcatcgg caacgtcctc tacaactggt cctattgcca tgacatctaa atccgaaaaa
     1561 agtggctctt cccctgcccc aaaataggca tcaccaatat ttcgcccccc taccagcgtc
     1621 accacgccat cgacagtgaa acttttattg tgcatacggc gattgagacg ggaaaagtcg
     1681 gtgatataac caagcggacg tagcaagcga aacgagaaag gattaaaaag ccggacttca
     1741 atgcggggat gactgtcaag caagcgtaaa atgtcgtcaa gtccgggcgt attgttgtcg
     1801 tccagcaaca aacggacgcg aacgccacgc tttgctgcgg ctaacagggc ggaaaagagt
     1861 aaccgacccg acatatcgtc ctgccagatg taatactgaa catccagcgt atgctctgcc
     1921 atttcggcca gacgataccg ggcggcaaac gcatccagac ttttctccag aggaaaaatg
     1981 ccacactgac cgggatgttg cgaacacagt ggcagcaccg cactcgccag ccggggcaaa
     2041 tcattcatct ccttgttgag taaggagtct ttcgtagagg tgggcgtttt cttcatcata
     2101 acagacaaag tatacctgtt cgggtaaagc gtgacgggta ataaattctg aaacggtttt
     2161 tactgcgatt tcagccgctg ccgcacgagg gtaaccataa actccagtac tgattgcagg
     2221 aaaagccact gacgtatagc tgtttgctgc caccagtcgc aggctattga gataggcatc
     2281 ctgcaaaagc tggtcttcgt tttgttcacc accacgccag actggcccga cggtgtgcac
     2341 tacggctttc gcgggaagat cgcctgcaag cgtaataacg gcatgccccg tagggcaatc
     2401 gccctgctgc tgcctgactt ttaaacaggc atccagcagg gccggacccg ctgcgcgatg
     2461 aatggcccca tcgacgccgc cgcctcccat taatgacgga ttagccgcat tcacaatcac
     2521 atcaacggcc agtttggtaa tatcaccctg cacaacatga atacgcgttt tcataacgcc
     2581 tccttacacc ttcttgtttc ttaagtgtac agcaggcttt ttggttaaat cgcatttttc
     2641 tgctcagcaa cgtgagttgt tcagaaactt agttatattc gatgttcatt acagccagct
     2701 ttttctgttt atcaagatac tgcactttta ctgaggctat ttgcgcgttt ttgacattgc
     2761 cgctcattaa cgctttgctg gaataggttc tgcccggaac agagccattc tgtgggcagt
     2821 tcatttctac gggtgatgac tgggtgctga cgtcacatgc cggttccaca atttggccat
     2881 aaaaatggat cacgccccct tgcgcggtgc ttcctgcgat ggcaataaaa ggaaaaaaca
     2941 tactgccgag cattagagag tctaaaaaag gtctgaacat ttattctatt tcctcaatga
     3001 ttagtcatcc ttgagggttg tgttatccat actttcaggt tggctgatcc gcagcagacc
     3061 attctctcca gattcatctt atgctcgata tttcaacaaa ttaagacttt tctgaagagg
     3121 gcggccattg ttgtgataaa tgaagtgact gtccatcaga aacagtaaca actattttca
     3181 cccgatcgtc cggggaaata tttaaactca acttcgtcaa agcaatgggt tgattagcag
     3241 gcaatgagag ggtcttttct tgcttcgtct gactttgccc tgaactgcct tcgcgcaggg
     3301 acaatatttg tactctgcac agacaagatt gagtaagagt gacttcagga ataatggtgt
     3361 acatatcccc ttgctgggtc gtattaaagg ttatctgact ggaaagtgcc gcaaggagta
     3421 ataacgcatt catattcttc tcccgaaaaa aaacagggct tgcgccctgt ttctttaata
     3481 cagaggatgt attagtactg atgagcggtc gcgttgttac caaagccaac ctgagtgacg
     3541 ttgacgctgg agttagatgc agtctggtca acagcagcac cgttaccacc accgaactgt
     3601 ttaacagtca tagtagaatc tttaccattc cactgatcaa gagtagcgct gttgccgaaa
     3661 ccacgctgag tcagatcgat tgagctgtca tcagaacctt ggccaacatc tgcgccgtta
     3721 ccgccgccgt gctgggtaat ggtcagatca gagttacggg cgtcagcttg cagagcaaga
     3781 gcagagttac cgccaccgta ctggtaaatg ttcagctctg aatttgggcc gctattatta
     3841 ccgccaccac cgtggttacc gccgccgccg tactgaggaa caacacctgc cagagcgcta
     3901 ccagagaata cgattgctgc aattgctgct acttttaaaa gtttcatgta aaacccccat
     3961 cggattgatt taaaagtcga atggaaatta acgttgtgtc acgcgaatag ccatttgcga
     4021 ctgtctctgc actacaattg ccgttttttg agtaccatac tgtgtaatat ttgctttatt
     4081 accagaacct ttctggataa tcatcgcagt attaccataa gcaccttgcg aaatactggc
     4141 atcgttggca ctgcccgcct gatcaatata tgcaaggtta taatctcctg tctggtcaat
     4201 ctttgcccgg ttgctactac cttcttgcgc aacaaccgcc aaaagttttg agcctccctg
     4261 ccgtaactga gcactattat tagtcccagc ttgaccaatt atggctgcct gattaaatga
     4321 agacttactc aattcattta ccgcgaagtt atattctgaa ttagctaaat cataacctgc
     4381 tgcggctgca atcccaggcg cacccagtat tgttaacatc ataaataaca at
//
LOCUS       DAZFLD010000282         4368 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.284, whole genome shotgun sequence.
ACCESSION   DAZFLD010000282 DAZFLD010000000
VERSION     DAZFLD010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4368
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.284"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            172..387
                     /gene="bdm"
                     /locus_tag="ACHKBL_004498"
     CDS             172..387
                     /gene="bdm"
                     /locus_tag="ACHKBL_004498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm-dependent modulation protein"
                     /protein_id="HFO5821253.1"
                     /translation="MFTYYQAENSTAEPALVNAIEQGLRAEHGVVTEDDILMELTKWV
                     EASDNDILSDIYQQTINYVVSGQHPTL"
     gene            489..626
                     /gene="sra"
                     /locus_tag="ACHKBL_004499"
     CDS             489..626
                     /gene="sra"
                     /locus_tag="ACHKBL_004499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary-phase-induced ribosome-associated
                     protein"
                     /protein_id="HFO5821254.1"
                     /translation="MKSNRQARHILGLDHKISNQRKIVTEGDKSSVVNNPTGRKRPAE
                     K"
     gene            783..2480
                     /gene="maeA"
                     /locus_tag="ACHKBL_004500"
     CDS             783..2480
                     /gene="maeA"
                     /locus_tag="ACHKBL_004500"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HFO5821255.1"
                     /translation="MEPKTKRQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLG
                     LLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPV
                     IYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILG
                     LGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNPRI
                     TDDEYYEFVDEFIQAVKQRWPDVLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAA
                     VTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIIAQTQREGLSEEAARQKVFM
                     VDRFGLLTDKMPNLLPFQTKLVQKRENLSDWDTDSDVLSLLDVVRNVKPDILIGVSGQ
                     TGLFTEEIIREMHKHCPRPIVMPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVV
                     WKDKIYPIAQCNNAFIFPGIGLGVIASGASRITDEMLMSASETLAQYSPLVLNGEGLV
                     LPELKDIQKVSRAIAFAVGKMAQQQGVAVKTSAEALQQAIDDNFWHAEYRDYRRTSI"
     gene            2614..3624
                     /gene="adhP"
                     /locus_tag="ACHKBL_004501"
     CDS             2614..3624
                     /gene="adhP"
                     /locus_tag="ACHKBL_004501"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HFO5821256.1"
                     /translation="MKAAVVTKDHHVDVTDKTLRSLKHGEALLKMECCGVCHTDLHVK
                     NGDFGDKTGVILGHEGIGVVAEVGPGVTSLKPGDRASVAWFYEGCGHCEYCNSGNETL
                     CRSVKNAGYSVDGGMAEECIVVADYAVKVPDGLDSAAASSITCAGVTTYKAVKLSKIR
                     PGQWIAIYGLGGLGNLALQYAKNVFNAKVIAIDVNDEQLKLATEMGADLAINSRTEDA
                     AKIVQEKTGGAHAAVVTAVAKAAFNSAVDAVRAGGRVVAVGLPPESMSLDIPRLVLDG
                     IEVVGSLVGTRQDLTEAFQFAAEGKVVPKVALRPLADINTIFTEMEEGKIRGRMVIDF
                     RR"
     gene            3810..4088
                     /locus_tag="ACHKBL_004502"
     CDS             3810..4088
                     /locus_tag="ACHKBL_004502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO5821257.1"
                     /translation="MIMNFRHKGLRDLFLLGKTSGVIPTQVKRLRHRLAVIDAASCLA
                     DIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVNGDAYILDYEDYH"
     gene            4088..>4368
                     /locus_tag="ACHKBL_004503"
     CDS             4088..>4368
                     /locus_tag="ACHKBL_004503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HFO5821258.1"
                     /translation="MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTG
                     KAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAEKTVDVSRLRRLVT"
BASE COUNT         1134 a         1065 c         1142 g         1027 t
ORIGIN      
        1 atcaactcat cacaataata atacctatca atatcatgga gttaaaatat aaaaatcatc
       61 aaccaggact aatcttaaca acgaagcggc aaatatttgc catgctgaat gtgctttata
      121 aatctgcgat ccgtagcaga caccataaat acacagacac ggagaatcac tatgtttact
      181 tattatcagg cagaaaattc aacagcagaa cccgctctgg ttaatgccat tgagcaaggc
      241 cttcgggcag agcacggtgt tgtcactgaa gatgacattc tcatggagct gaccaaatgg
      301 gtggaagcat ctgataacga catcctcagt gatatctacc agcaaacgat caattatgtg
      361 gtcagtggtc agcaccctac gctttaaggt gctatgcttg atcggcaacc taatttaggg
      421 gtttagcacg tgtttcttcg ctacggcgat gttgtcctta aaactagtaa caggattgag
      481 gagttaaaat gaaatcgaac cgtcaggcac gtcatattct tggactggac cataaaattt
      541 ctaaccagcg caaaatagtt accgaaggtg acaaatccag cgtggtaaat aacccaacag
      601 gcagaaaacg ccccgctgaa aagtaattca taaccatcag tcctcaatga cgattaaaca
      661 ccattgcctg cgcaatggtg tttttgtttt tatctgcttt atacttgggg ccgacgccct
      721 ggcggtaaag caaagacgat aaaagcgtgc cagggatgga tattcaaaaa agagtgagtg
      781 acatggaacc aaaaacaaaa agacagcgtt cgctttatat tccttacgct ggccctgtac
      841 tgctggaatt tccgttgttg aataaaggca gcgccttcag catggaagaa cgccgtaact
      901 tcaacctgct ggggttactg ccggaagtgg tcgaaaccat cgaagaacaa gcggaacgag
      961 catggatcca gtatcaggga ttcaaaaccg aaatcgacaa acacatctac ctgcgtaaca
     1021 tccaggacac taacgaaacc ctcttctacc gtctggtaaa caatcatctt gatgagatga
     1081 tgcctgttat ttatacccca accgtcggcg cagcctgtga gcgtttttct gagatctacc
     1141 gccgttcacg cggcgtgttt atctcttacc agaaccgcca caatatggac gatattctgc
     1201 aaaacgtgcc gaaccataat attaaagtga ttgtggtgac tgacggtgaa cgtattctgg
     1261 ggcttggtga ccagggcatc ggcgggatgg gcattccgat cggtaaactg tcgctctata
     1321 ccgcctgtgg cggcatcagc ccggcgtata cccttccggt ggtgctggat gtcgggacga
     1381 acaaccaaca gctgcttaac gatccgctgt atatgggctg gcgtaatccg cgtatcactg
     1441 acgacgaata ctatgaattc gttgatgaat ttatccaggc tgtgaaacaa cgctggccgg
     1501 acgtactgtt gcagtttgaa gacttcgcac aaaaaaatgc gatgccgtta cttaaccgct
     1561 atcgcaatga aatttgttct tttaacgatg acattcaggg caccgcggcg gtaacggtcg
     1621 gcacactgat cgcagccagc cgcgcggcag gtggtcaact gagcgagaaa aagattgtct
     1681 tcctcggcgc aggctctgcg gggtgcggca ttgccgaaat gatcatcgcc cagactcagc
     1741 gtgaaggatt aagcgaggaa gcggcgcggc agaaagtctt tatggttgat cgctttggcc
     1801 tgctgaccga caagatgccg aacctgctgc ctttccagac caaactggtg cagaagcgcg
     1861 aaaacctcag tgactgggat accgacagcg atgtgctgtc actgctggat gtggtgcgta
     1921 atgtaaaacc agatattctg attggcgtct caggacagac cgggctgttt acggaagaga
     1981 ttatccgtga gatgcataaa cactgtccgc gtccgatcgt gatgccgctg tctaacccga
     2041 cttcacgcgt ggaagccaca ccgcaggaca ttatcgcctg gactgaaggt aacgcgctgg
     2101 tcgccactgg cagtccgttt aacccagtgg tatggaaaga taaaatctac cctatcgccc
     2161 agtgtaacaa cgcctttatt ttcccgggca tcgggctggg cgttattgct tccggcgcgt
     2221 cacgtatcac cgatgagatg ctgatgtcgg caagtgaaac gcttgctcag tattcgccgc
     2281 tggtcctgaa cggcgaaggt ctggtactac cggaactgaa agatattcag aaagtctccc
     2341 gcgcaattgc ctttgcggtt ggcaaaatgg cgcagcagca aggcgtggcg gtgaaaacgt
     2401 ctgccgaagc tctgcaacag gccattgatg ataatttctg gcacgccgaa taccgcgact
     2461 accgccgtac ctccatctaa gcctgcgccc ggtggtgaaa gctgccgggc aattttcctc
     2521 tccctttttc agatctcatc catactgggt agtggcgaat aaatctcatt tgcctcacct
     2581 gctatgcaga acatcatccg aaaaggagga actatgaagg ctgcagttgt tacgaaggat
     2641 catcatgttg acgttacgga taaaacactg cgctcactga aacatggcga agccctgctg
     2701 aaaatggagt gttgtggtgt atgtcatacc gatcttcatg ttaagaatgg cgattttggt
     2761 gacaaaaccg gcgtaattct gggccatgaa ggcatcggtg tggtggcaga agttggccca
     2821 ggtgtcacct cattaaaacc aggcgatcgt gccagcgtgg cgtggtttta cgaaggatgc
     2881 ggtcattgcg aatactgtaa cagtggtaac gaaacgctct gccgttcagt taaaaatgcc
     2941 ggatacagcg ttgatggcgg gatggcggaa gagtgcatcg tggtcgccga ttacgcggta
     3001 aaagtgccag atggcctgga ctcggcggcg gccagcagca ttacctgtgc gggggtcacc
     3061 acctataaag cggtgaaact gtcaaaaatt cgtcccggtc agtggattgc tatctacggt
     3121 cttggcggtc tgggtaacct cgccctgcaa tacgcgaaga atgtctttaa cgccaaagtg
     3181 atcgccattg atgtcaatga tgagcagtta aaactggcaa ccgaaatggg cgctgattta
     3241 gcgattaact cacgcactga agacgccgcc aaaattgtgc aagaaaaaac cggtggcgct
     3301 cacgccgcgg tggtgacagc ggtagctaaa gctgcgttta actcggcggt tgatgctgta
     3361 cgagcaggcg gtcgtgttgt ggctgtcggt ctgccgccgg agtctatgag cctggacatc
     3421 ccacgccttg tgctggatgg tattgaagtg gtcggttcgc tggtcggcac gcgccaggat
     3481 ctaaccgaag ccttccagtt tgccgccgaa ggcaaagtgg tgccgaaagt cgccctgcgt
     3541 ccgttagcgg acatcaacac catctttacc gagatggaag aaggcaaaat ccgtggccgt
     3601 atggtgattg atttccgccg ctaagaggcc tttgctgcga ctgccatgtt cgggtcgcag
     3661 cctgttttgc gagacgcttt ccgcaatttc actcttcttc ttttcctccc ccatatttcc
     3721 cctcacaatt cgcaaaaacg tagcgaaatt tgttgcgctg cacggaacaa tagtgatagt
     3781 gttccttttg aaggaacaag gatgaagaga tgataatgaa tttcagacac aagggattgc
     3841 gtgacttgtt tcttctcggc aaaacctccg gcgttatacc gacgcaagtc aaacgattac
     3901 gccaccgact cgctgtgatt gatgcagcaa gctgtcttgc tgatatcgat atgcccggtt
     3961 accgactaca cccgttaagc ggcgatcgcg atggaatttg ggcgatatct gtctcgggca
     4021 actggcgaat cacatttgaa ttcgtcaatg gcgatgcata catactggat tacgaggact
     4081 atcactgatg aaaatggcca atcatccccg cccgggggac attattcagg aatcactgga
     4141 cgaacttaat gtcagcctgc gcgagtttgc cagagcaatg gaaattgcgc cctcaacggc
     4201 aagccgattg ctgaccggaa aagcggcgct cactccggaa atggcgatta aactttccgt
     4261 ggtgatcggc agttcgccgc aaatgtggct gaatctgcaa aacgcctgga gcctggcaga
     4321 ggctgaaaaa acggtggatg tgtcgcgact gcgccgtctg gttacgca
//
LOCUS       DAZFLD010000283         4333 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.285, whole genome shotgun sequence.
ACCESSION   DAZFLD010000283 DAZFLD010000000
VERSION     DAZFLD010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4333
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.285"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            2..826
                     /gene="yfdQ"
                     /locus_tag="ACHKBL_004504"
     CDS             2..826
                     /gene="yfdQ"
                     /locus_tag="ACHKBL_004504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdQ family protein"
                     /protein_id="HFO5821259.1"
                     /translation="MSQNLDATAINQIHALISAQGVNEIISKIGADAVALPENFRIHD
                     LEKFNLNRFRFRGALSTASIDDFTRYSKDLADEGTRCFIDADNMRAVSVLNLGTIDEP
                     GHADNTATLKLKKTAPFSALLSVNGERNSQKSLAEWIEDWADYLVGFDANGDAIQATK
                     AAAAVRKITIEANQTADFEDNDFSGKRSLMESVEAKTKDIMPVAFEFKCVPFEGLKER
                     PFKLRLSIITGDRPVLVLRIIQLEAVQEDMANEFRDLLVEKFKDSKVETFIGTFTA"
     gene            954..1490
                     /gene="yfdR"
                     /locus_tag="ACHKBL_004505"
     CDS             954..1490
                     /gene="yfdR"
                     /locus_tag="ACHKBL_004505"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HFO5821260.1"
                     /translation="MSFIKTFSGKHFYYDKINKDDIIINDIAVSLSNICRFAGHLSHF
                     YSVAQHAVLCSQLVPQEFAFEALMHDATEAYCQDIPAPLKRLLPDYKWMEEKIDAVIR
                     EKYGLPPVMSTPVKYADLIMLATERRDLGLDDGSFWPVLEGIPATEMFKVIPLSPSHA
                     YGMFMERFNELSELRKCA"
     gene            1481..1822
                     /locus_tag="ACHKBL_004506"
     CDS             1481..1822
                     /locus_tag="ACHKBL_004506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000812190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5821261.1"
                     /translation="MRMNVFEMEGFLRGKCVPRDLKVNETNAEYLVRKFTEAEAKISA
                     LAAENELARKAVQAFCDVVGDNTEVIAEEVGRDGVLVILEAMKATGNMPATDAFLAEV
                     RAQGGIWLVTR"
     gene            1804..1935
                     /locus_tag="ACHKBL_004507"
     CDS             1804..1935
                     /locus_tag="ACHKBL_004507"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5821262.1"
                     /translation="MARNAMIDFVDGEVGPNKNVPGLIRGTEICVSIAEQLRKGGNQ"
     gene            1932..2276
                     /locus_tag="ACHKBL_004508"
     CDS             1932..2276
                     /locus_tag="ACHKBL_004508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HFO5821263.1"
                     /translation="MSGINYQALREAAQNYQSMLAWYQEKPDSPNAEQDCDAALAAFK
                     REIRHREVDIIADLLDELEEAKQRIDEQESRTVKLPEPFKLAKSSSGLTYYYADEVNA
                     ALTSAGIRIEGE"
     gene            2278..2630
                     /locus_tag="ACHKBL_004509"
                     /pseudo
     CDS             2278..2630
                     /locus_tag="ACHKBL_004509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000208065.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2627..2773
                     /locus_tag="ACHKBL_004510"
                     /pseudo
     CDS             2627..2773
                     /locus_tag="ACHKBL_004510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311037.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3413..3655
                     /locus_tag="ACHKBL_004511"
     CDS             3413..3655
                     /locus_tag="ACHKBL_004511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFO5821264.1"
                     /translation="MFSLIQRGQIYTDRAGYPVVITRSTEHLVFFRRMDGHSGRVRIS
                     EFNNHFEHIDHQEFRQIQTELEQEAHLKKLRAMKRK"
     gene            3811..4026
                     /locus_tag="ACHKBL_004512"
     CDS             3811..4026
                     /locus_tag="ACHKBL_004512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000050710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFO5821265.1"
                     /translation="MALNIDCVPITTWCRDMGETLDAVNKRLQRGVWKEGVQVLNVEG
                     SKERWIDLTEVSKWARKNKYPYHSQEE"
     gene            3981..>4333
                     /locus_tag="ACHKBL_004513"
     CDS             3981..>4333
                     /locus_tag="ACHKBL_004513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3596 domain-containing protein"
                     /protein_id="HFO5821266.1"
                     /translation="MGEKKQISLSLPRGITIRQYKNCSTLVVTFTYKGVLCREPLPRL
                     EANVKGIKYAERLLGEIKNQISDNSFDFSKYFPNSRKLALFGMVKKNKSVRDYLEEYL
                     EICKNRKLSPSTING"
BASE COUNT         1234 a          879 c         1119 g         1101 t
ORIGIN      
        1 tatgtctcag aacttagacg caaccgcaat taatcaaatc catgccctta tttctgctca
       61 gggtgttaat gaaattatta gtaagattgg tgccgatgct gtggcattgc ctgagaattt
      121 ccgcattcat gatctggaaa aatttaattt aaatcgcttc cgtttccgtg gtgcgctttc
      181 cactgccagc atcgatgact ttacccgtta ttctaaagat cttgcagatg aaggcacccg
      241 ctgctttatc gatgctgata atatgcgtgc cgtcagtgtg cttaacctgg gtactattga
      301 tgaaccaggt cacgcagata acaccgccac tctcaaactg aaaaagacag caccgttctc
      361 tgctctgttg tctgttaatg gcgagcgtaa ctcccagaag tcactggcag aatggattga
      421 agactgggcc gactaccttg tgggctttga tgctaatggt gacgccattc aggcaacaaa
      481 agcggctgcg gcagtccgta aaatcacaat tgaagcgaac cagactgctg attttgaaga
      541 caatgacttc agcggcaaac gctccctgat ggagtctgtc gaagcgaaga ccaaagacat
      601 tatgccagtg gcatttgaat ttaaatgcgt tccgtttgaa ggcctgaaag aacgtccatt
      661 taaattacgc ctcagcatta tcactggcga tcgtcctgta ctggttctgc gcattattca
      721 gctggaagcg gtgcaggaag atatggctaa cgaatttcgt gatctgcttg ttgagaaatt
      781 caaagacagc aaagtagaaa cctttattgg tactttcacc gcctgatttc attactgcaa
      841 atgcccctgc gggggcattt atggaaacgt aattaactca ataatcgccg gatggtgagg
      901 acttcctttt accagaattc agcgcggtgc agcgcatata cgtggagaac aaaatgtcat
      961 ttattaaaac tttttccggg aagcattttt attatgacaa gataaataaa gacgacatca
     1021 ttattaacga tatcgcggtt tccctttcaa atatctgtcg ctttgcagga catctttcac
     1081 acttctacag cgtcgcccaa catgcagtgc tttgcagcca gctggtaccg caggaatttg
     1141 cttttgaagc gttaatgcat gatgcaacag aagcttattg tcaagacatc cccgcgccac
     1201 tgaaacgcct tcttcctgac tataaatgga tggaagaaaa aatagacgcc gtaatccgtg
     1261 agaaatacgg gttacccccg gttatgagca cgcctgtgaa atatgccgat ctcatcatgc
     1321 tggcaaccga acgccgcgat ctcgggcttg atgatggctc tttctggcct gtactggaag
     1381 gtatcccggc gacagagatg ttcaaagtta ttccactgtc accaagccat gcctacggga
     1441 tgtttatgga acgttttaac gagttatcgg agttacgcaa atgcgcatga atgttttcga
     1501 aatggaaggg tttcttcgcg ggaaatgtgt accgcgagat ctgaaagtga acgaaacaaa
     1561 tgctgagtac ctggtacgta aatttactga agcggaggct aagatttcgg cgcttgcggc
     1621 ggagaatgag ctggctcgta aggcagttca ggcattctgc gatgttgttg gcgacaacac
     1681 cgaggttatc gctgaggagg ttgggcgaga tggcgttctg gttattttgg aggccatgaa
     1741 ggcaacagga aatatgccag ccaccgatgc ttttctggct gaagtgcggg cacagggggg
     1801 gatatggctc gtaacgcgat gattgatttt gttgatggtg aagttgggcc aaacaagaac
     1861 gttccggggc tgattagagg cacagagata tgcgtaagta ttgctgaaca gcttcgtaaa
     1921 ggaggcaacc agtgagtggg attaattatc aggcgctgcg tgaggcggca caaaactatc
     1981 agtcgatgct ggcgtggtat caggagaaac cagacagtcc aaacgctgag caggattgtg
     2041 atgcagcttt ggctgcgttt aagcgtgaaa tccgtcatcg tgaagtggat attatcgctg
     2101 atttgctgga tgaactggag gaagcaaaac agcgtatcga cgagcaggag tcccgcacag
     2161 tgaagttacc agaaccattc aagctggcta aatcatcaag tggattaacg tactactacg
     2221 ctgatgaggt caacgccgca ctgacttctg ctggtattcg catagaagga gagtgagatg
     2281 accactatta ccaaagagcg actgcaatgg ctggctaaca tttctggccg cgatgacatt
     2341 gacgatatag acgtcggtga aattcgtgag ctggcgctta tcgcactggc atcgctggaa
     2401 gcagagcctg tggcgtcatg cattattgaa gatggggcat gtgtgttgac gggttcggtg
     2461 agtatgttgg tcactcgctg cctgatggaa cgcaccagct atacgccgcc ccgccagtgc
     2521 ctgataagtt gccgcgtgaa tacataagag gttggcctct tgcgcatagt gattatgcag
     2581 aaggatggaa cgattgccgt gctgcaatgc ttaatggaga taaatcatga ttgatcgaac
     2641 caaactggag cacattctcg aatatgccag gcagcagaaa tgtattgggc aactttgtaa
     2701 aattccacca ggagatatgc ttgaaatcgt ggaaatggcc atgcgtaagg ctggcaactc
     2761 tccggtaact ccggctcgcc tgcctggtgg tttcaccatt gaggatgcga aggagttata
     2821 tgaagacctg gtacgcagcc acataagcaa ggctttaagt ggcgaaaaga tgaagaagaa
     2881 ggagcgcgat gctgatttgc gctggatcca tggggttata gtccaggcag cctggtttgt
     2941 aaaagcatca ctggagcaga atgcgctaac gggtaactct caggtaatcc ctgatgacgg
     3001 tcgcgaacag ttcgaaacac ttatcaggtt ccatgcagat gataaaaacc atgaaacatt
     3061 attgcttcgt gctaacgaag ggatgaatta ccgggatcca aatgtggatt tggcgtggat
     3121 attctggaaa tccagtcgag agcacatatt gccgggaaaa agcgacaata atccagcatt
     3181 aggcgatcag gtgagtgaat taactatgtt ggttaagcga ttagccagtt cattaaaaag
     3241 cgtcaataaa tcaagcaagc tacctgatac ggcgatggag tatctgaaac agaatgggct
     3301 gataaacaca gagagtattt tacgatgacc tgacctgaag tgttctcaaa tgttggtatt
     3361 gcgttggcga ttgtgctggt tgtgtactca atttgccgct gggggtaaat acatgttctc
     3421 tctgattcaa cgtggtcaga tatacacgga cagagccgga taccctgtgg tgattactcg
     3481 cagcactgag cacttagtgt tctttcgacg catggatgga cactccggtc gggtgcgcat
     3541 cagtgaattc aacaaccatt ttgaacatat tgaccatcag gaatttcgcc agatacagac
     3601 agaactggag caggaagctc acctgaaaaa attacgtgcc atgaaaagga agtgaagagt
     3661 aaataaagca tttgaactat gggtccacca gcgttatggc agtcgctatg acctgacgcg
     3721 agatgttgac ggtttctact gccgtgaagt ggtgaaacga atgtttgaat gtggtgccac
     3781 tgccgtggat gaaagtttta tgaggtaact atggctctca acattgattg tgtacctata
     3841 acgacatggt gtcgagatat gggagaaaca ctggatgccg ttaacaaacg gttacaacgt
     3901 ggagtatgga aggaaggggt tcaggtttta aatgtggaag gctccaaaga acgatggatt
     3961 gatttaactg aggtttcaaa atgggcgaga aaaaacaaat atccttatca ctcccaagag
     4021 gaataaccat tcgtcaatac aagaattgct ctacattagt tgtaaccttc acatataaag
     4081 gggttctttg cagagaaccc ttaccaaggc tggaagctaa cgtaaagggg attaagtatg
     4141 ctgaacgact tctcggagag ataaaaaacc aaatcagtga taactcattc gatttttcaa
     4201 aatactttcc aaactccagg aagttggcat tatttggaat ggtaaagaag aataaaagtg
     4261 tgagagatta cctggaagag tatttggaaa tttgcaaaaa cagaaagtta tcgccttcaa
     4321 caataaatgg ata
//
LOCUS       DAZFLD010000284         4298 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.286, whole genome shotgun sequence.
ACCESSION   DAZFLD010000284 DAZFLD010000000
VERSION     DAZFLD010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4298
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.286"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(3..344)
                     /locus_tag="ACHKBL_004514"
     CDS             complement(3..344)
                     /locus_tag="ACHKBL_004514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001720563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RAQPRD family integrative conjugative element
                     protein"
                     /protein_id="HFO5821267.1"
                     /translation="MFSFPASSCRAGRLAGAAGMLFLLSFALPAQAAEKDELATVMRQ
                     LDHVQAALERARTASLQASSQGERYFFDYLRARADLQTIRDGIDHYLAPSRAQPRSSA
                     AVTGDYRRERP"
     gene            457..1023
                     /locus_tag="ACHKBL_004515"
     CDS             457..1023
                     /locus_tag="ACHKBL_004515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5821268.1"
                     /translation="MDEGKQPGTPGMNDIISIIILLTVYLIGLIFIILTINDIDRSSK
                     KRKYEKKLKEAIINGSLQNNDICLLAERWSASHEHIKSALLSVFNDFLTDNNFSHHVP
                     RVRELITWYEKYAPFTELPEDIKQQLQNIISLPDVSESEVQLLATSLHDIYNSCQKKE
                     KLARRINYVLGAVSIASFCISLFQILFK"
     gene            complement(1407..2168)
                     /locus_tag="ACHKBL_004516"
     CDS             complement(1407..2168)
                     /locus_tag="ACHKBL_004516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03747 family integrating conjugative element
                     membrane protein"
                     /protein_id="HFO5821269.1"
                     /translation="MAEPRREVNRAAQPSPPRQHGLLWNLLWGWPWMLVGILFSSLLL
                     SLLVEYIGIAFFWPEQGAAHSEAVMKTELGWLSSEFTRSLLLSTPSVTVTEWIATAYQ
                     WLFVETGFLSWLKQQNAGAVVGNSVSQYSEDVGRWLSHYLYEYLMATVYVTVVTLIRV
                     TILVLSVPLFVMVILIAVVEGLGRRDLRRYGAAYESSFVYHHAKKSIKPAIYIPCILY
                     LSWPTSVYPNLLIWPAAMLLGVAITVTMASFKKYL"
     gene            complement(2161..4257)
                     /gene="traD"
                     /locus_tag="ACHKBL_004517"
     CDS             complement(2161..4257)
                     /gene="traD"
                     /locus_tag="ACHKBL_004517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001510361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system coupling
                     protein TraD"
                     /protein_id="HFO5821270.1"
                     /translation="MSNRYVIEALLRPAVELNTAVVAATAAGVCVTAPWAVALAPSVS
                     YVTAAGFGVLAVVRFRQGMKIIRYRRNLRRLPRYVMSSRQIPVSRQRLFLGRGFRWTQ
                     KHTQRLQDTLRPEVAHYLQPGSLYRTARWLEMKTERSLPWIGQLLRRDSPLNPVRPLP
                     PVGGNPALHGIEPDEQDVTLALGERVGHTIVYGTTRVGKTRLAELLVTQDIRRGDVTI
                     VFDPKGDADLLLRVWAEAHRAGRGDELYIFHLGWPEISARYNAVGRFGRVSEVASRVA
                     GQLSGEGNSAAFREFAWRFVNIVARALVALGERPDYTLIMRYVNNIADLYIRYAGKVI
                     SEQMPELENAILNNMNVLGEADVPRTMQNQPDAVRIWAIEVALSSEAGKKLYDPILDG
                     LRSAVRYDRTYFDKIVASLLPLLEKLTTGKTAELLAPDYLDMSDTRPVFDWEQVIRKK
                     AVVYIGLDALSDSEVASAVGNSMFADLVSVAGHIYKHGMNAGLPGGNGTKNPVNLHCD
                     EFNELMGDEFIPLINKGGGAGMQVTAYTQTSSDIEARTGSAAKTAQVQGNFNNMIMLR
                     VRENRTAELLTTQLSQVEIYTKTLVSGHQDTADVTTGQDFTSSTQDRVGTVKVPLLEP
                     ADIVTLPKGQAFALLEGGQLWKIRMPLPATDADDALMPESIAKIAEAMRRNYHTGEGW
                     WNETPSVNMPEGEQNG"
BASE COUNT         1079 a         1261 c         1020 g          938 t
ORIGIN      
        1 catcagggac gctcccgccg gtagtcaccg gtcacggctg cggaagaccg gggttgcgcc
       61 cgggaagggg ccagataatg gtcaataccg tcacgaatgg tctgcagatc tgcacgggca
      121 cgcaaataat caaaaaagta gcgttccccc tgtgaagaag cctgcagaga ggccgttctg
      181 gcccgctcca gcgctgcctg cacgtggtca agctggcgca ttacagtggc cagttcatcc
      241 ttttcggcag cctgcgcagg taatgcaaag gacaaaagaa acagcatccc cgcggcaccg
      301 gccagacggc cggcacggca ggatgatgcc ggaaaagaaa acatacgcac ctccgtcaga
      361 gttaaacagt gcgtacagga tgcgtaaggc aggagactgg atcagcgata aactctattt
      421 gcaaaacaga aaatttttac gggagaatac actggtgtgg atgaaggcaa acaacccgga
      481 actcctggca tgaacgacat catcagtata atcattctcc tgacagtcta tctcatcggc
      541 ctgatattca ttatcctgac tatcaatgat atagacagat catcaaaaaa gagaaagtac
      601 gagaaaaaac tgaaagaagc gataataaat ggcagtttac aaaataatga tatctgttta
      661 ctggccgaac gctggtctgc cagtcatgaa catatcaaaa gcgcactgct gtctgttttc
      721 aatgattttc tgactgataa caacttttca catcacgtcc cccgggtcag agaactgatc
      781 acatggtacg agaaatatgc cccattcaca gaactccctg aggatataaa acaacagtta
      841 caaaatataa tcagtttacc cgatgtcagt gagtcagaag tgcagttatt agccacgtca
      901 cttcatgaca tttataattc ctgtcagaaa aaagagaagc tggccagaag aataaactat
      961 gttctggggg ctgtcagcat agccagtttc tgcatctcgc tgttccagat tctgttcaaa
     1021 taaatttatt ccagcctgaa tgatttattc ctgtttatta aaacacggca tcactactgt
     1081 ttcccgttaa tgacacctca ttaacaattt aaagaggaaa cacatcagca tggaatttct
     1141 ccgtactgcc cttccgttct gggccctgtt ccttctgacc tgggtttgtg ttgtgtattt
     1201 tttcatttat gaagaacctc gttgcccccc gtggatctat gacgtcatca gggccatcac
     1261 tctgttcatt attttcgtca acattgttgt cattccggcg ctgtcgttac tctgacactg
     1321 acgcgcactg accgtaaggg cgtggcttgt ctgccgacac cctgccacaa aacaagtttt
     1381 gcatcagggt gtccggcctc tgtggattat aaatatttct tgaaggatgc catggtcacg
     1441 gtgatggcca cccccagcag catggccgcc ggccatatca gcaggttcgg atacaccgac
     1501 gtcggccagg acaggtaaag aatgcaggga atatatatcg ccggtttaat agactttttt
     1561 gcatggtgat aaacaaagct ggactcatac gcagcaccat accggcgcag atcacgacgc
     1621 cccagtccct ccacaaccgc gatcagtatc accatcacaa acagcggaac agacaacacc
     1681 aggatcgtca cccgtatcag tgtgaccacc gtgacatata ctgtggccat caggtactca
     1741 tacagataat ggctcagcca gcgccccaca tcctcactgt actgtgacac actgttaccc
     1801 accacggcac cggcattttg ctgcttcagc cacgacagaa aacctgtttc cacaaacagc
     1861 cactgatacg ccgttgctat ccattctgtc accgttaccg atggtgtcga cagcagcaga
     1921 ctgcgggtga attccgaaga cagccacccc agttctgtct tcatcactgc ctcactgtgt
     1981 gctgccccct gctccggcca gaaaaaagca atcccgatat actcaaccag caggctcagc
     2041 agcagggagg aaaacaggat cccgaccagc atccacggcc agccccacaa cagattccag
     2101 agcagtccgt gctgacgtgg cggagagggt tgtgctgctc tgttcacctc acgacggggt
     2161 tcagccattc tgctctcctt ccggcatgtt cacggaggga gtctcattcc accatccctc
     2221 tccggtgtgg taattacgcc gcatggcctc tgcaattttt gcaatactct ccggcatcag
     2281 cgcgtcatct gcatcagttg caggcagggg catacggatt ttccacaact gtcccccttc
     2341 cagcagagca aaggcctgtc ctttcggcag ggtcacgata tctgccggct ccagcaaagg
     2401 caccttcaca gtaccaacac gatcctgggt actggatgtg aagtcctggc cggtcgtgac
     2461 atctgcggta tcctgatgac cggaaaccag cgttttggta taaatttcca cctgggacaa
     2521 ctgggtcgtc agtaactcgg ccgtccggtt ctccctgacc cgcagcataa tcatgttatt
     2581 aaagttaccc tgcacctggg ccgttttggc ggcactaccg gtacgggcct caatatcaga
     2641 agaggtctgc gtgtaagccg tcacctgcat accggcaccg cctcccttgt tgattaacgg
     2701 aatgaactca tcgcccatca gttcattaaa ctcatcacaa tgcaggttaa ccggattttt
     2761 ggtaccattc cctcccggaa gaccggcatt cattccgtgt ttgtagatat gcccggcgac
     2821 actcaccaga tccgcaaaca tggagttacc cacggcactg gccacctcac tgtcactcag
     2881 cgcatcaaga ccaatgtaca ccaccgcctt tttacgtatc acctgctccc agtcaaacac
     2941 gggacgggta tcgctcatat ccaggtagtc aggtgccagc agttctgccg tctttcctgt
     3001 ggtcagtttt tccagcaacg gcaacaggga tgccacaatt ttgtcaaaat aggtacgatc
     3061 ataacgtacc gcgcttcgca gaccatccag tatcggatca tagagtttct ttccggcttc
     3121 actgctgaga gccacctcaa tggcccagat acgcacagca tccggctggt tctgcatggt
     3181 ccggggaaca tccgcttccc ccagtacatt catgttattc agaatggcgt tttccagttc
     3241 cggcatctgt tcgctgatca ccttaccagc ataacggata taaaggtcgg caatattatt
     3301 cacataacgc ataatcagcg tgtaatccgg gcgctccccc agtgccacca gtgcacgggc
     3361 aacaatgttg acaaaccgcc atgcaaactc acggaaggcc gcactgttac cttcaccgga
     3421 taactgaccg gccacacggg aagccacctc cgacacacga ccaaaacgcc ccacggcgtt
     3481 ataacgcgca gagatttccg gccagcccag atgaaagata taaagctcat cgcccctgcc
     3541 ggcacggtgt gcttcagccc agacacgcag caacagatcc gcatcccctt tgggatcaaa
     3601 gacaatggtg acatctcccc gacgaatatc ctgtgtgacc agcaactccg ccagccgggt
     3661 tttcccgaca cgggtggtgc catagacaat ggtgtgcccc acacgttcac ccagtgccag
     3721 cgtcacatcc tgctcatccg gctcaatacc atgcagggca ggatttcctc ccaccggcgg
     3781 caggggacgg acgggattca gcggcgagtc acgacgaaga agctgcccaa tccagggaag
     3841 actgcgctcc gttttcattt caagccagcg cgctgtccgg tacagactgc ccggctgcag
     3901 atagtgcgca acctccggtc gcagcgtgtc ctgaaggcgc tgggtgtgct tctgtgtcca
     3961 gcggaagccg cgtcccagaa acagccgctg acggctgacc ggaatttgcc gggaactcat
     4021 cacataacgg ggcagccggc gcagattgcg acggtaacgg atgatcttca tcccctgtcg
     4081 gaaacggacc actgccagaa cgccaaagcc ggcggctgtg acatagctga ccgaaggcgc
     4141 gagcgccacc gcccagggcg ctgtcacaca gacaccggcc gccgttgctg ccaccacggc
     4201 ggtattcagt tccacagccg gacgcagcag agcctcaatc acataacggt tactcatgac
     4261 cgggtacctc tctgtaaaag acgccgccac catgagcg
//
LOCUS       DAZFLD010000285         4292 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.287, whole genome shotgun sequence.
ACCESSION   DAZFLD010000285 DAZFLD010000000
VERSION     DAZFLD010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4292
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.287"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1186
                     /gene="ybaT"
                     /locus_tag="ACHKBL_004518"
     CDS             <1..1186
                     /gene="ybaT"
                     /locus_tag="ACHKBL_004518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFO5821271.1"
                     /translation="QAALLMEASTWVAFAFGGIVAMFSGYAYARLGASYPSNGGIIDF
                     FRRGLGNGVFSLALSLLYLLTLAVSIAMVARAFGAYAVQFLHEGSQEEHLILLYALGI
                     IAVMTLFNSLSNHAVGRLEVILVGIKMMILLLLIIAGVWSLQPAHISVSAPPSSGAFF
                     SCIGITFLAYAGFGMMANAADKVKDPQVIMPRAFLVAIGVTTLLYISLALVLLSDVSA
                     LELEKYADTAVAQAASPLLGHVGYVIVVIGALLATASAINANLFAVFNIMDNMGSERE
                     LPKLMNKSLWRQSTWGNIIVVVLIMLMTAALNLGSLASVASATFLICYLAVFVVAIRL
                     RHDIHASLPILIVGTLVMLLVIVGFIYSLWSQGSRALIWIIGALLLSLIVAMVMKRNK
                     TV"
     gene            1217..1314
                     /locus_tag="ACHKBL_004519"
                     /pseudo
     CDS             1217..1314
                     /locus_tag="ACHKBL_004519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308571.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1311..1718
                     /gene="cueR"
                     /locus_tag="ACHKBL_004520"
     CDS             1311..1718
                     /gene="cueR"
                     /locus_tag="ACHKBL_004520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HFO5821272.1"
                     /translation="MNISDVAKITGLTSKAIRFYEEKGLVTPPMRSENGYRTYTQQHL
                     NELTLLRQARQVGFNLEESGELVNLFNDPQRHSADVKRRTLEKVAEIEQHIEELQSMR
                     NQLLALANACPGDDSADCPIIENLSGCCHHRAG"
     gene            complement(1719..2449)
                     /locus_tag="ACHKBL_004521"
                     /pseudo
     CDS             complement(1719..2449)
                     /locus_tag="ACHKBL_004521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000898666.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2521..2727)
                     /locus_tag="ACHKBL_004522"
     CDS             complement(2521..2727)
                     /locus_tag="ACHKBL_004522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000664217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5821273.1"
                     /translation="MKEQRKIPLTHIMIIGAFIFAFLQVVLLASLVHAVNVNNEIQEG
                     LFQSGRIMVESLQHILSVQTGING"
     gene            complement(2724..3146)
                     /locus_tag="ACHKBL_004523"
     CDS             complement(2724..3146)
                     /locus_tag="ACHKBL_004523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5821274.1"
                     /translation="MMNSSIKSFSLLAVILLAGCSSPASRIADCQAQGVSHDTCYLAE
                     QQRQTAILSASEAQAFKNAEAAQHAQAAKKAIYKGFGMTFRMSSKNFAYLNDSLCAID
                     EDNADATVYQSGLYNVIVYHHTGKVALMKEGQFVGYLK"
     gene            complement(3232..4248)
                     /locus_tag="ACHKBL_004524"
     CDS             complement(3232..4248)
                     /locus_tag="ACHKBL_004524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HFO5821275.1"
                     /translation="MKTVNVALLALIISATSSPVVLAGDTIEAAATELSAINSGMSQS
                     EIEQKITRFLERTDNSPAAYTYLTEHHYIPSETPDTTQTPTVQTDPDAGQKTVAATGD
                     TQTPARYQSMINARQSAVTDAQQTQITEQQAQIVATQKTLAATGDTQNTAHYQEMINA
                     RLAAQNEANQRTTTEQGQKMNALTTDVAAQQQKERAQYDKQMQSLAQKSVQAHEQIES
                     LRQDSAQTQQQLSNTQKRVADNSQQINTLNNHYSSLKNEVEDNRKEANAGTASAIAIA
                     SQPQVKTGDVMMVSAGAGTFNGESAVSVGTSFNAGTHTVLKAGISADTQSDFGAGVGV
                     GYSF"
BASE COUNT          957 a         1033 c         1119 g         1183 t
ORIGIN      
        1 gcaggctgcg ttgctaatgg aagcctcgac ctgggtcgcc tttgcttttg gcggtattgt
       61 ggcgatgttt tccggttatg cctatgcgcg cctgggggcg agctatccca gcaatggcgg
      121 tattatcgac ttctttcgtc gcggattagg caacggcgtc ttttcgctgg cactctcgtt
      181 attgtacctg ttgacgctgg cggtgagcat cgccatggtc gcccgtgctt ttggcgctta
      241 tgccgtgcag tttttgcatg aaggcagtca ggaggagcac cttatattgc tctacgcgtt
      301 ggggatcatt gcggtgatga cgcttttcaa ctccttaagc aaccatgcgg tagggcggct
      361 ggaagtgatc ctcgtcggca ttaaaatgat gatcctgtta ttgctgatca ttgccggtgt
      421 ctggtcgctg caaccggcgc atatttccgt ctctgcgccc cccagctccg gtgcgttctt
      481 ctcctgtatc gggataacct tccttgccta tgcgggcttt ggcatgatgg cgaacgcggc
      541 ggataaagtg aaagatccgc aggtcattat gccacgggcg tttctggtgg cgattggcgt
      601 taccacgttg ctttatatct cgctggcgct ggttttgctt agcgatgtat cggcattaga
      661 gttagaaaaa tatgccgata ccgccgtagc gcaggctgct tctccgctgc tcgggcatgt
      721 gggttatgtg atcgtcgtca tcggcgcttt actggcgacg gcttcagcca taaacgcgaa
      781 cctgttcgcc gtgtttaaca tcatggacaa catgggcagc gaacgcgaac tgccgaagct
      841 aatgaataaa tccctgtggc ggcagagtac ctggggcaac atcattgtcg tggtgctgat
      901 tatgctgatg acggcggcgc tgaatttagg ctcactcgcc agcgttgcca gcgccacctt
      961 tttgatctgc tatctggcgg tgtttgtggt ggcgatccgc ctgcgtcatg atattcacgc
     1021 ctcgttgccg attcttatcg ttggtacgtt ggttatgttg ttggtgatcg ttggctttat
     1081 ctacagtctc tggtcccagg gcagccgtgc gctgatatgg attattggcg cactcttact
     1141 cagccttatt gtggcaatgg tcatgaagcg caataaaact gtataacatc tctctgtgcg
     1201 cagtacctcc tgtattattg tggtggcggt cgatattcgc actggcaaaa aaacgtgctt
     1261 gaatatctgt tgaaaccctt taacaaagca caggaggcgt tgcgcgaacg atgaacatta
     1321 gcgatgtagc aaaaattacc ggcctgacca gcaaagccat tcgcttctat gaagagaagg
     1381 ggctggtgac gccgccgatg cgcagcgaaa acggttatcg cacctacacg cagcagcatc
     1441 tcaacgaact gaccttactg cgccaggcac gccaggtggg ctttaacctg gaggagagcg
     1501 gcgaactggt gaatctgttt aacgatccgc agcgtcacag cgccgacgtc aaacgtcgta
     1561 cgctggagaa agtggcggag atcgaacagc atatcgaaga actgcaatcc atgcgtaacc
     1621 agctgctggc gctggcgaat gcctgccccg gtgatgacag cgccgactgc ccgattatcg
     1681 aaaatctctc cggctgctgt catcatcggg cggggtgatt aaggcttgcg aaaatagtcg
     1741 tccattattt tcgtcagctc cggacgttcc ccgtcatcat tgatggcaaa atccagaaaa
     1801 aaagcatctg cttgcggcgt tcggttttgc gccatcaacc ggaaggtgag ggggcctgac
     1861 caggtttcgg atgggctgat gtgcttatca gggccgaaac tctcccatgc gttgggcggt
     1921 aatgttttgc acgcggtgag ttttggtatg tggtattgca gctctgtttt gtgcagacat
     1981 tccccgccag gaaataccag ggtttgttgc tcatcattaa aatgggttgc cgtgctgagc
     2041 accagtgttt tgagcggtcg cccttccagc gcggcggcag tagccttatg gtgtccgtcg
     2101 agcagaacgc agagatagga atcagggaga taccaggcca gagcgcgggc gattccagtt
     2161 ttcgagagca gacaaaaacc gggcggctcc ctgttcaccc tccgcaaaac cgctgtagac
     2221 aaattccgca caggtagggc aaccagcctc ggaccagaaa cgtattaacg ggacatcctt
     2281 gccagcagag cgaatggata agaccgtgcg agtgcggatc gtaaatcaca cattgacgca
     2341 gaaatccttt cccggacttc accgaaaaaa tgacgcccgc agcggtattt ttcagcagac
     2401 gtacttcagg ttttcgaaag gggaaaaagc atggccactc cttagccaaa atttcagttc
     2461 cctgaactgt gccagaaaat tacgctggtg actattgctc tggaaatgga aacagtgtcc
     2521 ttacccatta atccccgttt gtaccgaaag aatatgttgc aaactttcta ccataatgcg
     2581 ccccgactga aataagcctt cctggatttc attgttaaca ttcacagcgt gaaccaggga
     2641 ggctaataac accacttgca agaaggcaaa aataaacgca ccgataatca taatatgcgt
     2701 cagggggatt ttcctttgct ccttcatttt aaataaccca caaactggcc ttctttcatt
     2761 aaggcgactt ttcctgtgtg atgataaaca atgacattat acagacccga ctgataaacc
     2821 gtggcatcgg cattatcttc atctatggca cataatgaat cattgagata agcaaaattc
     2881 ttactactca ttctaaaggt catgccaaat cctttataaa tggctttttt tgccgcctgg
     2941 gcatgttgtg cggcttctgc atttttaaat gcctgcgctt cagaagcact taaaatagcc
     3001 gtctggcgct gctgttctgc gagataacag gtgtcatggc tgacgccctg cgcctgacaa
     3061 tctgcgatgc gggaagcggg tgagctacag ccagccagta atataaccgc cagcagggaa
     3121 aaggatttaa tggagctatt catcattgtt aaccttaaat aatgtgcgaa ttaatgaaga
     3181 ggtgaaaccc ggcaagcttc cttgctctta tttatattga ggattgaaat attagaacga
     3241 atatcctacg ccgacacctg cgccgaaatc agattgcgta tccgcagaaa taccggcttt
     3301 aagtaccgta tgcgttccgg cattaaaaga tgttccgaca gacaccgcag attcaccgtt
     3361 gaaggttccc gctcccgctg acaccatcat cacgtcaccg gttttaacct gtggttgtga
     3421 ggcgatagcg atggcagatg cagttcccgc attggcttct ttacggttgt cctcaacttc
     3481 gtttttcagc gaactgtaat ggttattgag cgtgttaatt tgttggctgt tatctgcgac
     3541 ccgtttttgc gtgttgctta actgttgctg cgtttgtgcg gaatcttgtc tcagactttc
     3601 aatttgctca tgtgcctgga cagacttctg cgccagactt tgcatttgtt tatcgtattg
     3661 agccctttct ttttgctgtt gtgctgccac atcggttgtc agtgcattca ttttctgccc
     3721 ttgttccgtg gtagtgcgct gattagcctc attttgagcc gccagtctgg cattaatcat
     3781 ctcctgataa tgcgcagtat tttgcgtatc tccagtcgcg gcgagcgttt tttgtgtggc
     3841 tacgatctgc gcctgttgct ctgtaatttg cgtttgctgg gcatcagtta ccgcagactg
     3901 tcgggcgttg atcatgctct gataacgggc aggtgtctgt gtatcacctg tagcggcaac
     3961 ggttttttgt cctgcgtcag gatctgtctg gacagtggga gtctgagtgg tatcaggtgt
     4021 ttcagaaggg atgtagtgat gttcagtcaa ataggtatac gcagcggggc tgttgtctgt
     4081 gcgttctaaa aagcgggtaa tcttctgctc aatctccgat tgcgacatgc cagagttaat
     4141 ggctgaaagc tctgttgccg ccgcttcaat ggtatcacca gctaaaacaa cagggctgga
     4201 tgttgctgaa attatgagtg ccagtaaagc tacgtttaca gttttcattc attttctctt
     4261 gttcaaaata agtcgcatta atgtttctat aa
//
LOCUS       DAZFLD010000286         4283 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.288, whole genome shotgun sequence.
ACCESSION   DAZFLD010000286 DAZFLD010000000
VERSION     DAZFLD010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4283
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.288"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..622)
                     /locus_tag="ACHKBL_004525"
     CDS             complement(<1..622)
                     /locus_tag="ACHKBL_004525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000200223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ImpA family N-terminal
                     domain-containing protein"
                     /protein_id="HFO5821276.1"
                     /translation="MTSEVKLKTGGDPRSLPDYAALRDEISKLTHPARPDVDWRYVET
                     LCLRLYEHNGVELQTASWYTIARMHTAGLSGLNEGLALIVALTRHHWSVMWPLNTHAR
                     LEIITGLFNRLQKTLRAMPPDDRDNLPLLYQTETFLKALSDTLARHELKQGSKVALPE
                     AMVKGYITRLENQPVQGEASSPVTLPAQALRSDAPDVQEHQTLPHSR"
     gene            complement(677..982)
                     /locus_tag="ACHKBL_004526"
     CDS             complement(677..982)
                     /locus_tag="ACHKBL_004526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001604127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5821277.1"
                     /translation="MNPLFNDIQMRLFYLNHSPYSWHWNVRFRPQEAVYIGSDTCHIT
                     ITCNQSGFHLTRDGQRLFTERYIRNLNELLPVLKRRWDVTPAIIRAVEYLSRVPVLH"
     gene            complement(1048..2793)
                     /locus_tag="ACHKBL_004527"
     CDS             complement(1048..2793)
                     /locus_tag="ACHKBL_004527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001529578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HFO5821278.1"
                     /translation="MTPGERRVASRLESFLNDDCLVWYDIPVGRKNRHPDFVIIDPEN
                     GLVFLEVKDWTVSTLRQVNQEQVTLETDGLLKSEINPLVQVRRYACDTVNALPANPCL
                     RQNDGQYKGRLNLAWAYGVVFTRITRQQLKTLAGNDENAVEKIFPSAQTICQDEMTQS
                     VLPEVFQQKIAGMFTTGFRTRVTPRMRDILRAHLFPEVTVKQNSQIKIMDIQQEILAR
                     NIGDGHRVIHGVAGSGKTMILLFRCLYLAETTPGKILVLCYNITLASYLRECIEARGL
                     ASRVTVSHFHSWCASMVKRHGIKVTADRKEYPDKCFSALEEAVNSGKITRTGYDAVLV
                     DEGHDFDSRWLALIARLFDNASRSLLLMYDDAQSIYRRERALNFSLASVGIQAQGRTS
                     VLPVNYRNPKRILHFAYAFSREYFEKHQNREIPLVQPQAGGEEGTEPEILRCASERDE
                     ARQVADWLEKRHALCGHWSDMAVLCPTERSAQQLQDVMTLRGVPFATCFDREGKKTYS
                     RRKDVVHLLMYQSSKGLEFPYVAVINASFVHSGAADESEVIPVLYVAFTRATRELLVT
                     CYRENSISRHLEDFA"
     gene            complement(3028..4230)
                     /locus_tag="ACHKBL_004528"
     CDS             complement(3028..4230)
                     /locus_tag="ACHKBL_004528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFO5821279.1"
                     /translation="MILVCVILWGIYVAVRALINLNERFIDAVSSPAGIIGLFFGLLT
                     VFAILLRFFIYRRLRKKTAAFEQAVSELVQRERGFNETVNAVIARGIRQEKEQLARRR
                     EEFHTARQKASRAMQRIVDSAWKFKAKTLLAGVTINNWQSKYDQLRKEREAYAAVSEK
                     IAFLNLEDNSDWESVRQQFLDKVALLEKAQEEKEYQAELKRQIREEKERQDELDRRQR
                     EAEEEERRLAEQQKLIEEALRAAEGAHREELEKQRLELEQKIQEAHAQYERAKSMAQL
                     TKQGHVYIISNIGSFGEDVFKIGMTRRLEPMDRVKELSGASVPFDFDVHAMISCDDAP
                     ALEKTLHDSLEKYRINRINLRKEFFRVKLEKIINEVERHHGQMEYVADPAALQYLQSL
                     EYAENEAA"
BASE COUNT         1064 a         1169 c          960 g         1090 t
ORIGIN      
        1 gacgggagtg cggcagggtc tgatgttcct gcacatccgg agcatcagac cggagtgcct
       61 gagccggaag cgttaccgga gacgacgctt cgccctgcac aggctgattt tccaggcggg
      121 tgatatatcc cttcaccatg gcttccggta aagccacttt actgccctgt ttaagttcgt
      181 gccgggcaag tgtgtcactc agtgccttca ggaatgtttc ggtctgatac agcaggggca
      241 ggttgtcacg gtcgtccgga ggcatggccc gaagcgtttt ttgcagacgg ttgaacagac
      301 cggtaataat ttccagccgg gcgtgggtat tcagcggcca catcactgac cagtgatgcc
      361 gggtcagcgc cacaatcaga gccagccctt cattcagtcc acttaaccct gctgtatgca
      421 tccgggcgat ggtgtaccag gatgccgtct gtaattccac gccattgtgt tcatacagac
      481 gcaggcagag cgtttccacg taacgccagt ccacatccgg acgtgccggg tgggtcagtt
      541 tgctgatttc atcacgcagg gcagcataat ccggtaatga cctggggtca ccaccggttt
      601 tcagtttcac ttcagaagtc atcggcagat tcggtgttac tgtattaaag catgattaac
      661 agtgacgaaa agaatgtcag tgtaataccg ggacccggga aaggtattcc acggcacgga
      721 tgattgccgg tgtcacatcc caccgccgtt tcagaaccgg taacagctca ttaaggtttc
      781 tgatataacg ttcagtgaac agccgttgtc cgtccctggt taaatgaaaa ccggactgat
      841 tacaggtaat ggtgatatga caggtatcgc tgccaatata gacagcttcc tggggtctga
      901 atctgacatt ccagtgccag gagtacggag agtggttcag ataaaaaaga cgcatctgaa
      961 tatcattaaa taacgggttc atggcgaatt aaataaacct gataataaca gtatgatgaa
     1021 agtccggaat aaagaaataa tccggtatta tgcaaaatcc tcaaggtgac gactgatact
     1081 gttttcacgg taacaggtca ccagtaattc acgggttgcc cgggtgaacg ccacataaag
     1141 caccggaatc acttcagact catccgctgc accagagtga acgaaagacg cattgataac
     1201 cgccacatac gggaactcca gccccttgct gctctggtac atcagtaaat gcaccacatc
     1261 cttacgtcgc gagtatgtct tcttgccttc cctgtcaaaa caggtggcaa aaggcacccc
     1321 gcgtagtgtc ataacgtcct gcaattgttg cgctgaacgc tctgtcggac acagcacagc
     1381 catatcactc cagtgaccac acagggcatg gcgcttctcc agccagtctg caacttgtct
     1441 ggcttcatca cgttcagaag cacaacgcag gatttccggc tccgtaccct cttcaccgcc
     1501 cgcctgtggc tgaacgagcg gtatttcacg gttctgatgt ttttcaaaat actcgcgcga
     1561 gaatgcataa gcaaagtgca gaatgcgttt cggattacga taattgaccg gcaggacaga
     1621 agtgcgtccc tgtgcctgaa tccccacact ggccagagaa aagttcaggg cccgctcccg
     1681 ccggtaaatg gactgggcgt catcgtacat caggagcagt gaacgggatg cgttatcaaa
     1741 cagacgggca atcagggcca gccagcgact gtcaaaatca tgcccctcat ccaccagtac
     1801 agcatcatat ccggttcggg tgatttttcc gctgttcacg gcctcttcca gtgcactgaa
     1861 gcatttatca ggatattctt ttctgtcggc cgtcacctta ataccgtggc gttttaccat
     1921 tgaggcacac cagctgtgaa aatgggatac cgtaacccgt gacgccaacc ctctggcttc
     1981 aatgcattcc cgcagatagc tggccagggt aatgttgtag cacaacacca gaatctttcc
     2041 gggtgtggtt tcagccaggt aaaggcaacg gaacaaaaga atcatggttt ttccggaacc
     2101 ggccacacca tgaatgacac gatgcccgtc accaatattt ctggccagaa tctcctgctg
     2161 aatatccatg attttgattt gtgagttctg tttcacggtc acttcaggaa acagatgggc
     2221 gcgcagaata tctctcatcc ggggtgtcac ccgggttctg aatcccgtgg taaacatccc
     2281 ggcaattttt tgctggaata cctcaggtaa aacagactgc gtcatttcat cctggcagat
     2341 tgtctgtgct gaaggaaaaa ttttttccac cgcattctca tcattccctg ccagtgtctt
     2401 cagttgctga cgggtaatgc gggtaaacac cacaccatac gcccaggcca ggttcagccg
     2461 tcccttatac tgaccgtcat tctgccgtaa gcacgggttt gcaggtaagg cattaaccgt
     2521 atcacaggca taacgacgaa cctgtaccag cggattgatt tctgatttca gaagaccgtc
     2581 ggtttccagc gtgacctgct cctgatttac ctggcgcagc gtggaaaccg tccagtcctt
     2641 gacctccaga aataccagtc cgttctccgg gtcaataatg acaaaatccg gatgccggtt
     2701 tttccgtccc accggaatat cgtaccagac aaggcagtca tcattcagaa agctctccag
     2761 tcgtgaagcc accctcctct ccccaggcgt cattctctcc tcagtggagg ccgatacatt
     2821 tatcagtttc gccataaacg caccttaatc tgtgaacaaa aaataatcac caccgacatc
     2881 cacggatgtt atatcgtggg tgtcgcttta ttcagaaata aaaaacagaa tcattaacat
     2941 gaccagaata ctgtgaacca tgctgcaggg tatctgcaga tatcccttct gttatatccg
     3001 gccggggtaa aatcagaaca ctgactgtca ggctgcttca ttttctgcgt attccagact
     3061 ctgcaggtac tgcagcgccg ccgggtcagc cacatattcc atctgcccgt ggtgacgctc
     3121 cacttcatta atgattttct caagcttcac gcggaaaaat tctttacgca ggttaatcct
     3181 gttaatgcgg tatttttcca gactgtcatg aagtgttttt tccagcgcag gcgcatcatc
     3241 acaggaaatc atggcgtgaa catcaaaatc aaacggaaca gaagcaccac ttaactcttt
     3301 gaccctgtcc atgggttcca gccgtcgggt catgccaatt ttaaagacat cctcaccaaa
     3361 cgaaccaata ttcgaaatga tataaacatg cccctgtttc gtaagctgag ccatggattt
     3421 agcacgttca tactgcgcat gtgcttcctg aattttctgt tccagctcca ggcgttgttt
     3481 ctctaactcc tccctgtgag ccccttctgc tgcgcgcagc gcctcctcaa tcagtttctg
     3541 ctgctcagca agacgccgtt cttcctcttc cgcttcccgc tggcgccggt caagttcatc
     3601 ctgacgctct ttttcttcac gtatctgacg cttaagctct gcctgatatt ccttctcttc
     3661 ctgcgctttt tccagcaatg ccactttgtc cagaaactgt tgcctgacac tctcccagtc
     3721 agaattatct tccaggttaa gaaaggctat tttctcactg acagcagcgt aagcctctct
     3781 ttccttacga agctggtcat atttgctttg ccagttatta atcgttacac cagccagcag
     3841 agtcttcgcc ttaaatttcc aggcggaatc aacaatccgt tgcattgcac ggctggcctt
     3901 ctgtctggcg gtatgaaatt cctcacgacg tctggccagt tgctcctttt cctgtcgtat
     3961 tccccgggct atcacggcat tgacagtttc attaaaaccc cgctctctct gaaccagctc
     4021 tgaaacggcc tgctcaaagg ctgctgtttt ctttctgagt ctccggtaaa taaaaaaacg
     4081 aagtaaaatt gcaaatacag taagcaaacc aaaaaacaaa ccaataatac ctgccggact
     4141 cgatactgca tcaataaagc gctcattaag atttatcagc gccctgaccg ccacataaat
     4201 cccccagaga ataacgcaaa ctaaaatcag gacaaatccc gctgcattat catctttttt
     4261 cttcctgcca tgactttcct tta
//
LOCUS       DAZFLD010000287         4245 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.289, whole genome shotgun sequence.
ACCESSION   DAZFLD010000287 DAZFLD010000000
VERSION     DAZFLD010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4245)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4245)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4245
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.289"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1395
                     /locus_tag="ACHKBL_004529"
     CDS             <1..1395
                     /locus_tag="ACHKBL_004529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB family protein"
                     /protein_id="HFO5821280.1"
                     /translation="VLFKPWPGRLQCVIGHLAENEVRGELTFIEKAQGVHKARQIHEE
                     QLGRTVSLRELADLLTHEGLPIHNSTISRMEDALKYLYPWIPDLMESGLGRPQVTALL
                     ALRQDAERVWGQFAVAADTDAEFDRVFGESCRKFNSPELWSLEMFRDELIGDLLQALP
                     HPSLDYDRWLLELDPKERNRRHHFGEPEPVVVPPSVRGSSTVQPGAPVSGIRSEVPEC
                     QPDTDTEAMPESTVSIFPGTERKQHGITRPLKAVSDADTERTEKCSRPSQEESAGRGD
                     SGASSAENALRPEIQPDMYGGKPVFSGDVMEDGAAGAVLASVMTGDTAPGTTDALPAS
                     VTHFAVGRDDIEHLQNEAFRLAWELAESAGCAEEIAMDRESDLSAGFGAAVEKCSPVV
                     AFLAGLAGDAPRTVLPVSLKDLLTGGSAPEAWPLLDDDHAVKLLRLLSVLRRLRALQR
                     SVLPDDDEGDENDE"
     gene            1388..2113
                     /locus_tag="ACHKBL_004530"
     CDS             1388..2113
                     /locus_tag="ACHKBL_004530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2786 domain-containing protein"
                     /protein_id="HFO5821281.1"
                     /translation="MSDMSHQERLIRRVRKLLELSRNNSNAHEAGLALARAQQLMAKH
                     GITEQDAGLSSVQTSSSQGAPSDAEKVPAWMFRLVMVVTQTFGCRAYYDWRAVPGDRY
                     RRCVTFYGFSERPQVAAYAFDVLCRHLREATDAYLRTQSRRLKLSTRRARAAQFRDGW
                     VQGIRQVVMPFSVTEEESRLMRLWMEQQDMVTVDSREMKACRGGDLARWQGYEAGKNA
                     SLHHGVGESARQVVPGLMIREAS"
     gene            2110..2718
                     /locus_tag="ACHKBL_004531"
     CDS             2110..2718
                     /locus_tag="ACHKBL_004531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001065815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2857 domain-containing protein"
                     /protein_id="HFO5821282.1"
                     /translation="MNNSLSQATNGLLMQLVMDLKSGYLRRCESLGLAREEMQMLQGL
                     TIEEIHYLSNSEVSVIRLDINHENLLRMLQQARTEQKRLQRIDRALALGGSIELMAFY
                     FGLSSVDVAARRRIAGIDVRPGRGITLSDEESAELWRLWQKSGITDVESADGLDVMML
                     AAEQMNIPLTAVWHAVRGWHQGTAGFNDVSASRRKLNREKAE"
     gene            2733..3146
                     /locus_tag="ACHKBL_004532"
     CDS             2733..3146
                     /locus_tag="ACHKBL_004532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XF1762 family protein"
                     /protein_id="HFO5821283.1"
                     /translation="MAEINFQVLREAEQLTGVATAGRPVARHFDDGLTLEVNRTCTTG
                     ERNANSALYGAVWRAAKAMGYRRCITYTRADESGASLRAAGFVRVKELPSGKSWAESS
                     VALKSTRDPVGSGGVPRVLREIRRMSTTVIHIKGE"
     gene            3150..3617
                     /locus_tag="ACHKBL_004533"
     CDS             3150..3617
                     /locus_tag="ACHKBL_004533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5821284.1"
                     /translation="MAIAERTGISSRGDTYEIWLGANCYHYRSDGYAVLLKEGRNRAY
                     PCRVTSETIKSALAAIVELQQKLTAAEKKKECENHGFLLPAEMLIRCLEDQTGGRWIR
                     AHEPVMLMKRLISNLKKARPECTVPDEALAYLRRNCLVNMGDIPVTSRRMSDV"
     gene            3635..>4245
                     /locus_tag="ACHKBL_004534"
     CDS             3635..>4245
                     /locus_tag="ACHKBL_004534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010615558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STY4528 family pathogenicity island replication
                     protein"
                     /protein_id="HFO5821285.1"
                     /translation="MTLPVDSLIAYTLEKMQERIEQKGRQQDGKIQSGLLFTGNVHDA
                     IPRRLLLDTRLSPLDKMAWMMIRLYAQNNEGAVFPTYDELQLQLASPGKGKASRETVS
                     RVLLMLRITGWLSLCKRVRDEKGRVRGNIYAQHDEPVTCRDAEILDPRYLDMVADACM
                     SRNRTISQTARDILCEIKNDPGMRHYHSHISQLEARIGSAQTPG"
BASE COUNT         1003 a          971 c         1353 g          918 t
ORIGIN      
        1 gtgctgttta aaccctggcc gggtcggctc cagtgtgtga ttggtcatct ggctgaaaat
       61 gaagttcgcg gtgaactgac ttttattgag aaggcgcagg gggtccacaa ggcaagacaa
      121 atccatgagg aacaactggg cagaacagtg tctcttcgtg aactggctga tttgttaacc
      181 catgaagggt tgccgataca taactccacg atcagcagaa tggaagatgc cctgaagtat
      241 ctgtacccct ggatccccga cctgatggag tcaggactgg ggcgtccaca ggttacagca
      301 ctcctcgcac ttcgtcagga cgcagaacgt gtatgggggc agtttgctgt tgcagccgat
      361 acggatgcgg aatttgaccg cgtgttcggg gaaagctgcc ggaagtttaa ttcgccggaa
      421 ctgtggtcac tggagatgtt ccgcgatgag cttatcggag atttgttgca ggccctgcca
      481 catccctccc tggactatga ccggtggttg ctggaactgg atccgaaaga gcgaaaccgt
      541 cgtcatcatt ttggtgagcc ggaaccggtg gtggtgcctc cttcagtccg tggcagcagt
      601 acggtacaac ccggggcacc ggtatccggg atccggtctg aagtaccgga gtgtcaaccg
      661 gatacggata cagaggcgat gccggaaagc acagtctcca tattccccgg tactgagcgc
      721 aagcaacatg gtattacccg gccactgaaa gccgtttctg atgctgacac agagcgcact
      781 gaaaaatgca gtcgaccttc tcaggaagag agcgcaggca ggggggacag tggggcgtct
      841 tctgctgaaa atgccctgcg tcctgagata cagccggata tgtacggtgg taagccagtc
      901 ttcagtggtg atgttatgga agatggtgct gccggagcag tactggcatc agttatgaca
      961 ggagatactg ccccgggaac cactgatgct cttcccgcgt cggtgacgca ctttgctgtg
     1021 gggcgggatg atatcgaaca tctgcagaat gaagcgttcc gtctggcctg ggaactggct
     1081 gaatcagccg gttgtgctga agagattgcc atggacaggg aaagcgacct gtctgccggt
     1141 ttcggggcag cagtggagaa atgttctcct gtagtggcgt ttctggcggg gctggccggt
     1201 gatgcaccgc ggacagtcct ccccgtgagt ctgaaagacc tgcttacagg gggcagtgca
     1261 ccggaagcgt ggccactgct ggatgacgac catgccgtga agctgctgcg tcttctgagt
     1321 gtgctgcgcc gtctgcgagc cctgcagcgc agtgtgttac ccgacgacga tgagggggat
     1381 gagaatgatg agtgatatgt cgcatcagga acgtctgatc aggcgggtca ggaaactgct
     1441 ggagctgagc cgtaataaca gcaatgccca tgaggccggg ctggcgcttg caagggcgca
     1501 acagctgatg gcgaaacacg gtatcacgga gcaggatgcc ggactttctt cggtgcagac
     1561 ctcttcatca cagggggcac cttctgatgc tgaaaaagtt ccggcgtgga tgtttcgtct
     1621 ggtcatggtg gtgacacaga catttggctg ccgggcgtat tacgactggc gggcggtgcc
     1681 cggagaccgg tatcgccggt gtgtgacgtt ttacggtttc agtgaacgtc ctcaggtggc
     1741 ggcttatgcg tttgatgttc tgtgccgtca tctaagggaa gcaacggatg cgtatcttcg
     1801 tacacagagc cggagactga aactgagcac ccgtcgtgcc cgggcggctc agtttcgtga
     1861 tggctgggtt caggggattc gtcaggtggt gatgccattc agtgtgacag aagaggaaag
     1921 tcggctgatg cgtctctgga tggagcagca ggatatggtg acggtggact cccgtgagat
     1981 gaaagcctgt cgcggtggtg acctggcccg ctggcagggg tatgaggccg ggaaaaatgc
     2041 ctcactgcat catggtgtgg gggagagtgc ccgacaggtg gtgccgggtc tgatgatacg
     2101 ggaggcgtca tgaataacag cttgtctcag gcaaccaacg gtctgctgat gcaactggtg
     2161 atggacctca agagcggcta cctgcgtcgc tgcgaatctc tggggctggc ccgggaggaa
     2221 atgcagatgc tgcagggact gaccattgag gaaattcatt acctgtcaaa ctctgaagtg
     2281 tcggttatcc ggctggacat taatcatgaa aacctgctgc gtatgctgca gcaggcccgg
     2341 acggagcaga aacgtctgca gcgcattgac cgggcactgg cgctgggagg ctccattgaa
     2401 ctgatggcgt tttactttgg actttccagt gtggatgtgg cggcgcgccg tcgcattgcc
     2461 ggtattgatg ttcgcccggg acgtggcatc acactgagtg atgaggaaag tgcagagctg
     2521 tggcgtttat ggcagaagag cggcatcacg gatgtggaga gtgcggacgg actggatgtc
     2581 atgatgctgg cagctgagca aatgaatatc ccgctgacgg cggtatggca tgccgtgagg
     2641 ggatggcatc agggaactgc ggggtttaat gatgtatcgg ccagtcgccg gaaactgaac
     2701 cgggaaaaag cagaatgaca aggacaaatg acatggccga aattaacttt caggtactgc
     2761 gggaggctga acaattgaca ggtgtggcga cagccggcag acctgttgca cgccactttg
     2821 acgatgggct gacactggag gtcaatcgta cctgtactac tggtgaacgt aacgctaaca
     2881 gcgcacttta tggcgctgtc tggagggcgg caaaagctat gggatatcgc cgttgcatta
     2941 catacaccag ggctgatgaa tccggagcct ctcttcgggc agctggtttt gttcgtgtga
     3001 aagaacttcc ttcaggaaaa agctgggcgg aatcaagtgt cgccctgaag agtacacgcg
     3061 atccggttgg aagcggtggt gttcctcgtg tgctccggga aatcaggaga atgagcacta
     3121 ctgtcattca catcaaaggg gaatgagata tggctattgc agagagaacg ggtatctcct
     3181 cgcgcggcga tacttacgaa atctggttgg gagcaaactg ttatcactac cgctctgatg
     3241 gttatgcagt gcttctgaaa gaaggcagga atcgtgctta tccatgtcgg gtgacgagtg
     3301 aaacgattaa atctgcgctg gcagcaattg ttgaattgca acagaaatta actgcggcag
     3361 agaagaaaaa ggagtgtgag aaccacgggt ttcttttgcc agctgagatg ttaattcgct
     3421 gtctggaaga tcaaacaggg ggacggtgga tccgtgcaca tgaaccagta atgctgatga
     3481 agcgattaat cagtaacctg aaaaaagcca gaccagaatg taccgttcct gatgaagcgc
     3541 tggcatatct ccggcgtaac tgtctggtga atatgggcga tatacccgtg accagccgac
     3601 ggatgtctga cgtataacaa aaaggaaaag tgtaatgaca ttacccgtag acagcctgat
     3661 tgcgtacacg ctggaaaaaa tgcaagagcg gatagagcaa aaaggccgac aacaggacgg
     3721 aaaaatacaa agcggactgc tgtttaccgg aaatgtgcat gatgccattc cgcgtcgact
     3781 gcttctggat actcgacttt ctccgctgga taagatggcg tggatgatga ttcggctgta
     3841 tgcacagaat aatgaaggtg cagttttccc cacatatgac gaactgcaac tgcagctggc
     3901 ttccccgggg aaaggaaaag cctccaggga gactgtcagc cgggtgctgc tgatgctgcg
     3961 gatcacgggc tggctgagtc tgtgcaaacg ggtgcgtgat gagaaagggc gggtacgggg
     4021 caatatttat gcccagcacg atgaaccggt gacgtgccgg gatgctgaaa tactggatcc
     4081 acgttatctg gatatggtgg cagatgcctg tatgagccgg aaccggacca taagtcagac
     4141 ggcccgggat attctgtgcg aaataaaaaa tgatccggga atgcgtcatt atcacagcca
     4201 tatcagtcaa ctggaagccc ggatagggag tgcccagaca ccggg
//
LOCUS       DAZFLD010000288         4213 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.290, whole genome shotgun sequence.
ACCESSION   DAZFLD010000288 DAZFLD010000000
VERSION     DAZFLD010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4213
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.290"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..246)
                     /locus_tag="ACHKBL_004535"
     CDS             complement(<1..246)
                     /locus_tag="ACHKBL_004535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001480051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid transfer ATPase TraJ"
                     /protein_id="HFO5821286.1"
                     /translation="MISPDDFGTFPFSRFTAEEFRHFFAWCARHRVSDIDLTGGSPVS
                     VSRFGRRVRSSVNPLPSSIMMALSDELFGREVLPRVLS"
     gene            complement(230..1066)
                     /locus_tag="ACHKBL_004536"
     CDS             complement(230..1066)
                     /locus_tag="ACHKBL_004536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000848138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HFO5821287.1"
                     /translation="MKTHLCLLLSALLLTGCAGQHHRSSAVMTENAPPPDIRAWLTPS
                     GNQDDGISDTRRQMLTDAGRTLGFRGGKAQRSWELIQALNARESTLNALYDFRPLISK
                     EGWLPPVIDEAQDVAHIRPDQIRTASRVWTILRPERFVSNPPGWRDWLLKGLSTTATP
                     GTEGRVVPEDRAQRRLWENALRQGWQEGRLNADLTLEANQKRLTRDYRGMMLYALLWR
                     QGMITRPDVTEQMQTVTGNGQKLITGDRVRRLKTHAEFTLQKSRWRPLVSTEGEKNDI
                     TR"
     gene            complement(1063..1515)
                     /locus_tag="ACHKBL_004537"
     CDS             complement(1063..1515)
                     /locus_tag="ACHKBL_004537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001081199.1"
                     /note="Members of this family include DotD of type IVB
                     secretion systems and TraH of plasmid conjugative plasmid
                     systems, both lipoproteins; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DotD/TraH family lipoprotein"
                     /protein_id="HFO5821288.1"
                     /translation="MNRMIPAGCLAVLCLNGCHLPPDNTAPTRKYGAASDVAVARHAQ
                     YQLWGQGIYSAALPERQSGGSLKAHSDRVSFDWEGDALELLNELARLRGQQFSYSGVR
                     LPLPVSLHVRDMTFADVLRLTEAQTAWRATLSQSAGRLHLSFMTPEKK"
     gene            complement(1564..1734)
                     /locus_tag="ACHKBL_004538"
     CDS             complement(1564..1734)
                     /locus_tag="ACHKBL_004538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001597011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5821289.1"
                     /translation="MFNKNKTHIENKAFMVEPFRDPTANECNVVIANDICIRVALSPS
                     EVFQPFSKNTNQ"
     gene            complement(1874..3076)
                     /locus_tag="ACHKBL_004539"
     CDS             complement(1874..3076)
                     /locus_tag="ACHKBL_004539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001532112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraF"
                     /protein_id="HFO5821290.1"
                     /translation="MMMNMHITRTLIASAVLFSFNSTAATSYFEARNDAMGGTGVASS
                     HYGVAPLANPALLTKAGPEDDFSLLLPSVGGQLSDPGNISDNADKISDDWKAFDRAID
                     SQSNVSQTAARLKTRLQDFRHTHADAQVGTSAVATLPGDTLAAALMLKSYGTVSVDGK
                     VSDADLNYLESIANSNGQDVDKDRLTSQAFARAALVTDVGIALATELETAGQKWSLGF
                     TPKFQRVDLFNYNVLVKNYDSSAFKGDRYHNTRNGINADIGASMDLDDNWTLGLVAQN
                     LLPRSIETKEVNGVTETFRIRPQVTAGVSWHNDMFTTALDVDLTPASGFTSDSKRQFA
                     AIGAEFNAWKWAQLRAGYRQNLASNDGSAFTAGLGISPFDVVHIDVAGIAGTDNNYGA
                     VAQLQFTF"
     gene            complement(3166..3987)
                     /locus_tag="ACHKBL_004540"
     CDS             complement(3166..3987)
                     /locus_tag="ACHKBL_004540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HFO5821291.1"
                     /translation="MKPNSTGIAARMMLSIDRARISEALLNNRQLQSTPLQIRYPQGV
                     REALGIMSEQLSLSVSDLTRILVEDALTEMFLPAENIVHRLICRTEHIMQAHDVCVTT
                     MTTLLAPWNIRPAVFRDPDKLADYLTGEILTALADWFYLNPEWLNGRVRYPLSRAGEW
                     PATQDAFCQLISASDNVDIIIWHSFPFAGVHSQEYCGVLFRKKKEINKAIIYPVLSLS
                     PVRMNKEKENWFHTARNLYAKTSVRTVTLRPAQAEYLMTGKILPAELFSIPLLPW"
BASE COUNT          978 a         1023 c         1170 g         1042 t
ORIGIN      
        1 tgacagaacc cggggaagaa cctcccgccc aaacagctca tcactcagtg ccatcatgat
       61 ggaggacgga aggggattta cggatgagcg aacccgccgt ccaaagcgac tgacggatac
      121 cggtgaaccg ccggtcagat caatatcact gacgcggtgg cgggcacacc aggcaaaaaa
      181 atggcgaaac tcttctgccg taaaacggga gaaggggaag gtaccgaagt catctggtga
      241 tatcattttt ctctccttcg gtgctgacaa gagggcgcca gcgcgatttc tgcagcgtaa
      301 attcagcatg tgttttcagg cggcggacgc gatcgccagt gatgagtttt tgcccgttgc
      361 cggtaaccgt ctgcatctgc tctgtgacat ctggacgtgt gatcatgccc tgacgccaca
      421 gaagggcgta aagcatcatg ccccggtaat cacgggtcag gcgcttctga ttagcttcca
      481 gtgtgagatc tgcgttgagg cgcccttcct gccatccctg acgtaaggcg ttttcccata
      541 gtctgcgttg tgcccggtct tccggcacaa cccggccttc agtgcctggt gtggcggtgg
      601 ttgagagtcc tttcagcagc cagtcacgcc atccaggtgg attactgaca aagcgttccg
      661 ggcggaggat ggtccagaca cgggaggctg ttcttatctg gtccggtctg atgtgtgcaa
      721 catcctgcgc ttcatcgata acaggaggca gccagccttc cttgctgatg agaggccgga
      781 aatcatacag ggcattcagg gtactttccc gggcattcag tgcctgaatc agttcccatg
      841 agcgctgtgc ttttcctcca cggaatccca gcgttctgcc ggcatccgtg agcatctgac
      901 gacgggtatc tgaaatacca tcatcctggt ttccggaggg cgtcagccat gcacggatat
      961 ccgggggcgg tgcgttttct gtcatgacgg cagaagaacg gtgatgctgt ccggcacagc
     1021 ctgtaagcag gagagcagac agcaggagac acaggtgtgt tttcattttt tctccggcgt
     1081 cataaaagac agatgtaaac gcccggctga ctgcgagagt gttgcccgcc aggcagtctg
     1141 ggcttcagtg agacgaagga catcagcaaa ggtcatgtcg cggacgtgca gtgagacggg
     1201 taaggggaga cggacgccgc tgtagctgaa ctgctggccg cgcagacggg ccagttcgtt
     1261 gagcaattcc agggcatcgc cttcccagtc aaaggaaaca cggtcgctgt gagccttcag
     1321 actgccgccg gactgacgct ccggaagggc tgcagagtaa attccctgcc cccatagctg
     1381 atactgcgca tgccgggcga cggcgacatc agaggcagca ccatactttc tggtcggagc
     1441 tgtattatcc ggggggagat ggcagccgtt caggcagagt acggcgagac atcccgcagg
     1501 tatcatacgg ttcatggtgt atttttcctg agcagtgaaa gaagaaagcg ttgtggggag
     1561 gatttactgg ttcgtgtttt tgctgaatgg ctggaacacc tcagagggtg acagtgctac
     1621 ccggatacaa atatcgtttg ctatcacgac attacattcg ttagctgtcg gatcccggaa
     1681 cggttccacc ataaaggctt tgttttcgat atgcgtttta tttttgttaa acatgcattt
     1741 cctcatgcga aaatgatatg tcagataaca gccagatcag aggcagcact acaggtctgg
     1801 ctgtgtcgtt tgatggtcgg aaaacgcgag gatgcaggtc tggatacccc ggtcagtacc
     1861 ggggcagaga tgatcagaat gtgaactgca gttgtgcaac ggcaccgtag ttgttgtctg
     1921 tgcctgctat acccgccaca tcaatatgga cgacatcaaa tggtgagata cccagcccgg
     1981 cggtgaacgc actgccatca ttgctggcca gattctggcg gtagcctgca cgtaactgtg
     2041 cccatttcca ggcattaaat tcagcaccaa tggcagcaaa ctgacgttta ctgtctgatg
     2101 tgaagccact ggccggggtc aggtcaacat ccagtgctgt agtgaacatg tcgttgtgcc
     2161 aggagacacc ggcagtcacc tgcgggcgga tcctgaaagt ttcagtgaca ccgttcactt
     2221 cctttgtctc aatactgcgg gggagcaggt tctgtgcgac cagacccagt gtccagttgt
     2281 catccaggtc catgctggcc ccgatatcag cgttaatgcc gtttctggtg ttgtggtaac
     2341 ggtcgccttt gaaagcgctg ctgtcgtaat ttttaaccag cacgttgtag ttaaacaggt
     2401 caacacgctg gaattttggt gtgaagccca gagaccactt ttgtccggcc gtttccagtt
     2461 cagtagccag agcaataccc acatcggtga ccagtgcggc acgggcaaaa gcctgagagg
     2521 tcagtctgtc tttatccaca tcctgaccgt tactgtttgc aatgctttcc agatagttca
     2581 ggtcggcatc actgacttta ccgtcgacac tgaccgtacc gtagcttttc agcatcagag
     2641 cggcagcgag ggtgtcaccg ggaagagtcg ccacagcaga tgttcccacc tgtgcgtcag
     2701 catgcgtgtg acggaagtcc tgaagtcgcg tcttcagacg tgcagcagtc tgcgacacgt
     2761 tgctctgact gtcgatggca cgatcaaagg ctttccagtc atcactgatt ttgtctgcgt
     2821 tgtcactgat attacccgga tcggacaatt gcccaccaac ggacggaagc agaagactga
     2881 agtcatcttc cggaccggct tttgtcagca gggccgggtt tgccagaggt gccacaccgt
     2941 agtgagagga ggctaccccg gttcccccca ttgcatcatt gcgggcttca aaatatgaag
     3001 ttgcggctgt tgaattaaag gaaaataaaa cagcagatgc gatgagagtt cgggtaatgt
     3061 gcatattcat catcatttca gtttcctggt ttgaggtgtt ttgggtacag caacggtatg
     3121 acacgactgt gccattaaaa atttattggt tttttctctg cgttattacc agggtaataa
     3181 cggtatactg aataactcag caggtaatat ttttcctgtc atcagatatt ctgcctgtgc
     3241 cggacgaagc gttacggtcc ggacggatgt ctttgcatat aaattgcggg cagtgtgaaa
     3301 ccagttttct ttttccttat tcattcttac aggtgataaa gaaagtacag gataaatgat
     3361 ggctttatta atttcttttt tcttgcggaa cagtacaccg caatattcct gtgaatgaac
     3421 accggcaaaa ggaaaactgt gccagataat aatatccaca ttatcgctgg ctgaaataag
     3481 ctggcagaac gcatcctgtg ttgctggcca ctcaccggca cggcttaaag gataacgtac
     3541 cctgccgttc agccattccg ggttgagata aaaccagtca gccagagcag tcagaatctc
     3601 tcctgtcaga taatctgcga gtttgtccgg gtcacggaaa acggcaggac ggatattcca
     3661 gggagccagt aacgttgtca tcgttgttac gcagacatca tgtgcctgca tgatgtgttc
     3721 tgtccggcag ataagacggt gaacgatgtt ttcagccggg agaaacattt cagttaatgc
     3781 atcttcgacc aggatacgcg tcaggtcgga tacagaaagg gagagttgct cgctcattat
     3841 tccgagtgct tctctgacgc cctgcggata acggatctgc aggggagtgg actgcaactg
     3901 gcggttattg agcagagctt cgctaatccg ggccctgtca atggagagca tcatgcgggc
     3961 agcaatgcct gtgctgttgg gtttcatttc acctccgtgc ggttcatatg ttaagtctaa
     4021 agagcgtgtt tactgaacgg aaacgagtta tttcgatcgt tatgatcgag tgtgtttcaa
     4081 gagtgctttt aactctgttt taagcgtgaa tgaaaaggat tcggacagga cgtgatttag
     4141 catgccgctg gtctggcatt atgctgcaga gcagacggag tcaggagatt gaaagtattt
     4201 cgttatataa tct
//
LOCUS       DAZFLD010000289         4163 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.291, whole genome shotgun sequence.
ACCESSION   DAZFLD010000289 DAZFLD010000000
VERSION     DAZFLD010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4163)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4163)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4163
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.291"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            227..835
                     /locus_tag="ACHKBL_004541"
     CDS             227..835
                     /locus_tag="ACHKBL_004541"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HFO5821292.1"
                     /translation="MKLVGSYTSPFVRKLSILLLEKGITFEFINELPYNADNGVAQFN
                     PLGKVPVLVTEEGECWFDSPIIAEYIELMNVAPAMLPRDPLESLRVRKIEALADGIMD
                     AGLVSVREQARPAAQQSEDELLRQREKINRSLDVLEGYLVDGTLKTDTVNLATIAIAC
                     AVGYLNFRRVAPGWCVDRPHLVKLVENLFSRESFARTEPPKA"
     gene            933..2324
                     /gene="selA"
                     /locus_tag="ACHKBL_004542"
     CDS             933..2324
                     /gene="selA"
                     /locus_tag="ACHKBL_004542"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004125 - L-seryl-tRNA(Sec) selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HFO5821293.1"
                     /translation="MTTETRSLYSQLPAIDRLLRDSSFLSLRDTYGHTRVVELLRQML
                     DEAREVIRDSQTLPAWCENWAQEVDARLTKEAQSALRPVINLTGTVLHTNLGRALQAE
                     AAVEAVAQAMRSPVTLEYDLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLM
                     LAATASGKEVVVSRGELVEIGGAFRIPDVMRQAGCTLHEVGTTNRTHANDYRQAVNEN
                     TALLMKVHTSNYSIQGFTKAIDEAELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEP
                     MPQELIAAGVSLVSFSGDKLLGGPQAGIIVGKKEMIARLQSHPLKRALRADKMTLAAL
                     EATLRLYLHPEALSEKLPTLRLLTRSAEVIQIQAQRLQAPLAAHYGAEFAVQVMPCLS
                     QIGSGSLPVDRLPSAALTFTPHDGRGSHLESLAARWRELPVPVIGRIYDGRLWLDLRC
                     LEDEQRFLEMLLK"
     gene            2321..>4163
                     /gene="selB"
                     /locus_tag="ACHKBL_004543"
     CDS             2321..>4163
                     /gene="selB"
                     /locus_tag="ACHKBL_004543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HFO5821294.1"
                     /translation="MIIATAGHVDHGKTTLLQAITGVNADRLPEEKKRGMTIDLGYAY
                     WPQPDGRVPGFIDVPGHEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLT
                     GNPMLTVALTKADRVDEARVDEVERQVKEVLREYGFAEAKLFITAATEGRGIDALREH
                     LLQLPEREHASQHSFRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRV
                     RALHAQNQPTETAHAGQRIALNIAGDAEKEQINRGDWLLADAPPEPFTRVIVELQTHT
                     PLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDTPLWLADNDRLVLRDISARNTLAG
                     ARVVMLNPPRRGKRKPEYLQWLASLARAQSDADALSVHLERGAVNLADFAWARQLNGE
                     GMRELLQQPGYIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMAL
                     PMEDEALVLLLIEKMRESGDILSHHGWLHLPDHKAGFSEEQQAIWQKAEPLFGDEPWW
                     VRDLAKETGTDEQAMRLTLRQAAQQGIITAIVKDRYYRNDRIVEFANMIRDLDQECGS
                     TCAADFRDRLGVGRKLAIQILEYFDRIGFTRRRGNDHLLRDALLFPEK"
BASE COUNT          941 a         1048 c         1224 g          950 t
ORIGIN      
        1 gctcatcctt gttttatcgg gttatttgtc tgctgacagg cgaaacccta cagatttata
       61 agaaagcctt aagtgagata tctgtcagat aagaacaatt aggaaaatat ttatatcacc
      121 atgataaata tgtgatttat tgggaagtaa caaaccatca acattttctg ccctttttcc
      181 tatcaccagg ccatactgat atttttcctc gcacaaagga ctcggcatga aactcgtcgg
      241 tagctacacc agcccgtttg tacgcaaact ttctattctg ttgttagaaa agggcataac
      301 tttcgaattt attaatgaac tgccctataa cgcggataac ggcgtggcgc aatttaaccc
      361 gttagggaaa gtgccggtgc tggtgaccga agagggcgaa tgctggtttg attcgccgat
      421 catcgctgaa tatattgaat taatgaatgt cgctccggcg atgttgccgc gcgatccgct
      481 ggagtcgttg cgggtgcgca aaattgaggc actggcggat ggcattatgg atgccgggct
      541 ggtatcggtg cgtgaacagg cgcgtccagc ggcgcagcag tctgaagatg aattgttacg
      601 ccagcgcgag aaaatcaacc gcagtctgga tgtgctggaa ggatatctgg tcgatggcac
      661 gctcaaaacc gatacggtca atctggcgac tatcgccatt gcctgtgccg tcggatatct
      721 caatttccgc cgcgttgcgc caggctggtg tgtcgaccgc ccacacttag tcaaactggt
      781 cgaaaacctg tttagccgcg aaagttttgc tcgcaccgaa ccgccaaagg cttgatgcgc
      841 gatatgtcct cctgacccat ctcacgttac aatccgtggt tatgttaaac gcccttctcc
      901 gtgtgagagg gccttgatca gccaggtttc ctatgacaac cgaaacgcgt tccctctata
      961 gtcaacttcc ggctattgat cgcttattgc gcgatagctc cttcctttct ttgcgtgata
     1021 cttatggtca cacccgcgtg gtggaattgc tgcgtcagat gcttgacgaa gcgcgagaag
     1081 tgattcgtga tagccagacg ctgcccgcat ggtgtgaaaa ctgggcgcaa gaagtcgatg
     1141 cccggttgac gaaagaggcg cagagcgcgc tgcgtccggt gatcaacctg acgggaaccg
     1201 tgctgcatac caatcttggg cgagctttac aggcagaagc cgcggtggaa gccgttgcgc
     1261 aggctatgcg ttcgccagtg accctcgagt acgatctgga cgacgccgga cgcggacatc
     1321 gcgatcgggc gctggcgcag ctgctgtgcc gcattacggg agcggaagat gcctgtatcg
     1381 ttaataacaa tgcggcggcg gtgttattga tgttggcggc cactgccagc gggaaagagg
     1441 tggtggtatc tcgcggcgaa ctggtggaga ttggcggcgc gtttcgtatt cctgatgtca
     1501 tgcgtcaggc aggctgcacc ctacacgaag tggggaccac caaccgcacg cacgctaatg
     1561 attatcgtca ggcggtgaat gaaaataccg cgctgttgat gaaagtacat accagtaact
     1621 acagcattca gggattcacc aaagcgatag atgaagcgga actggtggcg ctcggcaaag
     1681 agctggatgt tcccgtagtg actgatttag gcagtggctc gctggttgat cttagccagt
     1741 acggtttgcc gaaagagcca atgccgcagg agttgattgc ggcgggcgtc agtctggtga
     1801 gtttctccgg tgacaagttg ttaggcgggc cgcaggcagg aattattgtt ggtaaaaaag
     1861 agatgatcgc ccgcctgcaa agccacccgc tgaagcgtgc attacgcgcg gataaaatga
     1921 ccctcgcggc gctggaagcc acgttgcgtc tttatttaca ccctgaagct ctgagtgaaa
     1981 aattaccgac cctgcgcctg cttacccgca gcgcagaggt cattcaaatc caggcacaac
     2041 gtttacaggc tccccttgcc gcacattacg gcgcggagtt tgcagtccag gttatgccat
     2101 gtctttcgca gattggcagt ggttcactgc cggttgatcg cctgccgagc gcggcattaa
     2161 cgtttacacc ccatgatgga cgcggtagcc accttgagtc attagccgcc cgctggcgtg
     2221 aattgccagt gccggtgatt ggtcgtattt atgacggacg attgtggctg gatttacgct
     2281 gccttgaaga tgagcaacgg tttttggaga tgttgttgaa atgattattg cgactgccgg
     2341 acacgttgac cacggcaaaa caaccttatt gcaggcgatt actggcgtaa atgccgatcg
     2401 tctgccggaa gaaaaaaagc gcggcatgac tatcgatctc ggctatgcct actggccgca
     2461 gccggatggc cgcgtgcctg gttttatcga cgttcccggt catgaaaagt ttctttccaa
     2521 catgctggcg ggcgtcggtg gtatcgatca cgcgctgttg gtggtggcgt gcgatgacgg
     2581 tgtgatggca cagacccgtg agcatctggc gattttgcag ctgactggta acccgatgct
     2641 gacagtggcg ctgaccaaag ccgatcgcgt ggacgaagcg cgtgttgatg aggttgaacg
     2701 ccaggtaaag gaggtgctgc gggaatacgg ttttgctgag gcaaaactgt ttatcaccgc
     2761 agcaaccgaa ggtcggggaa ttgatgccct gcgcgagcat ctgcttcagt taccagaacg
     2821 cgaacacgcc agccaacata gtttccgcct cgcgatcgac cgtgcattta ccgtaaaagg
     2881 tgccgggctg gtcgtcaccg gtacggcgtt aagcggggaa gtgaaggtag gcgattcact
     2941 ctggctgact ggtgtaaata aaccgatgcg cgtgcgtgcg ctgcatgcgc aaaatcagcc
     3001 aacagaaact gcccatgccg ggcagcgtat cgcgcttaac atcgcgggtg atgcggaaaa
     3061 agagcagatt aaccgtggcg actggctgct tgccgatgcg ccgccggagc cgttcacacg
     3121 ggtgattgtc gagcttcaaa cccatacacc gctgacccag tggcagccgc tgcatattca
     3181 ccacgccgcc agccacgtca cgggacgcgt ttcgctgctg gaagataacc ttgccgagct
     3241 ggtgttcgac acgccgctgt ggctggcgga taacgatcgc ttagtgttgc gcgatatctc
     3301 ggcccgcaac acgctggcgg gggcgcgtgt ggtgatgctt aacccgccgc gtcgcggtaa
     3361 gcgtaagccg gaatatctgc aatggctggc gtctcttgca cgggcgcaga gcgatgccga
     3421 tgcgttatct gttcatctgg aacgcggagc ggttaacctt gcggatttcg cctgggcacg
     3481 gcagctcaac ggcgaaggga tgcgcgaact gctgcaacag cctggctata ttcaggctgg
     3541 ttatagcttg ttgaatgcgc cggttgccgc tcgctggcag cggaaaattc tcgacacatt
     3601 agcgacttat catgagcaac atcgcgatga acctggtcct gggcgagaac gtctgcgacg
     3661 tatggcgttg ccaatggaag atgaagcgct ggtactgttg ctgattgaaa agatgcgcga
     3721 aagcggcgac atcctcagtc atcacggctg gctgcatctg ccggatcaca aagcgggctt
     3781 cagcgaagag cagcaggcca tctggcaaaa agcagagcca ctgtttggtg acgaaccgtg
     3841 gtgggtgcga gacctggcaa aagaaacggg aaccgacgag caggcaatgc gcctgacgct
     3901 acgccaggcg gcgcaacagg ggataattac tgcgatcgtc aaagatcgtt attaccgtaa
     3961 cgatcggatt gtcgaatttg ccaatatgat ccgcgatctc gatcaggaat gtggttcaac
     4021 ctgcgcggcg gatttccgcg atcgcttagg cgtaggccga aagctggcaa ttcaaatcct
     4081 ggaatatttt gaccgcattg gctttacgcg tcgtcgtgga aatgatcatt tattgcgcga
     4141 cgcattatta tttccggaaa aat
//
LOCUS       DAZFLD010000290         4027 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.292, whole genome shotgun sequence.
ACCESSION   DAZFLD010000290 DAZFLD010000000
VERSION     DAZFLD010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4027
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.292"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..817
                     /locus_tag="ACHKBL_004544"
     CDS             <1..817
                     /locus_tag="ACHKBL_004544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011192679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HFO5821295.1"
                     /translation="VAVLVPSLTDKTSSRFMQSLQQVLNKNEFQLLLGCHEYNQLKEA
                     EILMTLLQGNPAALVIFGSQLADKTHQILEKTNIPTINVVGSPFSPAQITIETAFFEA
                     SHKLTEHLLEQGYKNIGFIGAHMDNRLQRQQLNGWHKAMLSYYQNSDLVITMPGAASL
                     QLGRYALNEILLRQPELDAVICSHEEIALGIMFECQRRLLKIPGNIAVACLDGSDSCD
                     QTHPTLTSIRIDYKKMGTETGKLLIGLLNNNHDESEESRIVQFNYQIELRQST"
     gene            1020..1700
                     /locus_tag="ACHKBL_004545"
                     /pseudo
     CDS             1020..1700
                     /locus_tag="ACHKBL_004545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907654.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1717..2025
                     /locus_tag="ACHKBL_004546"
                     /pseudo
     CDS             1717..2025
                     /locus_tag="ACHKBL_004546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611881.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
     gene            complement(2128..3234)
                     /locus_tag="ACHKBL_004547"
                     /pseudo
     CDS             complement(2128..3234)
                     /locus_tag="ACHKBL_004547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001063816.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
BASE COUNT         1076 a          945 c          927 g         1079 t
ORIGIN      
        1 agtcgccgta ctggttcctt ccctgacaga taaaaccagc tcccgcttta tgcagtcgtt
       61 gcagcaggtg cttaataaaa atgaattcca gctactgctt gggtgtcatg agtataatca
      121 gctcaaagaa gctgaaatcc tgatgacgct tcttcaaggt aacccggcgg cgttagttat
      181 ttttggttct cagttggctg ataaaactca ccagatactc gagaaaacaa atatacctac
      241 cattaacgtt gtcggttccc ctttcagtcc ggctcaaatt accatcgaaa cagcattttt
      301 tgaagcatca cataaactga ccgaacatct gctggaacag ggttataaga atatcggatt
      361 tatcggtgcg catatggaca accgcctgca acgtcagcaa ctgaacggct ggcataaagc
      421 aatgctgagc tattaccaga actctgatct tgttatcacc atgccgggtg ccgcaagttt
      481 gcaattgggg cgttacgcat taaacgagat attactgcga caaccagagc tggatgctgt
      541 gatttgcagc cacgaagaga ttgcgctggg tatcatgttt gagtgccagc ggcgtttgtt
      601 aaaaatccct ggaaatatcg ctgtagcttg tcttgacggt tcagacagtt gcgatcaaac
      661 acacccaacc ttgacatcca tccgtattga ctacaaaaaa atggggacag agacgggtaa
      721 gctgctgatt ggtctattga ataataatca tgatgaatca gaagagtcgc gtatcgtgca
      781 attcaactac cagatagaat tacgtcagag tacatgagct caaaagggga ttattgcaaa
      841 gttagctatt gtggtaggga tagccgtttc cggctattcc ccgcgcagag ccggatgtgt
      901 gctgctaacg catcctgtca acgacggatg aaaagttatc cacttatatc tccaccaacg
      961 gcccaatatt gtatcgttcg tgacacatcc atttctactt caggtatttg gcgtagtagc
     1021 gggtgctaaa aatttgccgc aaaaccgcaa tatcagcccg cagaggcgct ctccagatta
     1081 tccattccta tattatctct gcaccggatg aattactcga acaactcaga aatatgacac
     1141 atatgcaact tatcaggact ctgggatcct ggaggcctga tgccagtgaa tacagtaatg
     1201 ttaccaacgt ttatcgcatt acattgaaat cccttgttcg acgctatttc gagtttcatg
     1261 atgaaatcac caacttggat gtcatgattg cggcaattgt tgatgaactg gcacctgaac
     1321 tgattaaacg taatgctgtt ggatacgaaa gcgctttgca gttgttgatc tctgccggag
     1381 acaatcccta acggttaaga accgaatcag gttttgcggc gttgcgcggt gtcagccagt
     1441 ttcccctatc ttctggaaaa acgaaccatt accgacttaa tcggggtggg gagcgtgttg
     1501 caaatagtgc actttacatc atcactatag gatgtttaca aactgacgat aaaacaaagg
     1561 aatatgtctc caggcgagta gccgaaggac atacaaaaat ggaagcaata cgctgtctga
     1621 agcgctatat ctcacgcgaa gtttatacat tactacgaaa tcaaaacagg catgttaact
     1681 gcatcccgat aacgtcttga ctcttagaag ggcgtccaag gcagtcatta ttcgagcagg
     1741 cagttccggc agttattgtg tcgataccag atcaggcaga gtatgagccg gcgcggaaaa
     1801 tgctgggata acagctcaat ggaacgcttc ttcaggagtc tgaagaacga atggatgccg
     1861 gtggtgggtt acgtaagctt cagcgaggca gctcacgcca taacggacga tatcgttgga
     1921 tattacagcg cactaagacc gcacgaatat aacagtgggt tatccccaaa cgaatcggaa
     1981 aatcgatact ggaaaaactc taactcggtg gccagttttt gttgaccact tcagccatcg
     2041 ccagacgcta acatgagtta ctcttgttct gggctacgtc accttcaggg aactggttat
     2101 cctcattctt tatttgtgac attgtggtta ttgtgcgaca aacatggcta tcccgcccca
     2161 tatcccagat aaaaccacct gctcacgtgc gacagcgaca attgtaatat tgacattctt
     2221 tcctttggcc tgaagttttc gagacctctt gcaaagcctg agttgagcat cccatgctct
     2281 gtcaatgacg gggcggggta aattttcctg attttttgta ttgccggact gatgcgtgtc
     2341 ggatgccggt aactccacgc tgcctcaacc aatagctttc tggcatagct gtttccggct
     2401 ttggtaatgc tgccctgatg tctgcttcca cccgatgaat attctgatgg cgtgatacca
     2461 aaccatgaca tcagttgtcg tggatgttca aaacgggtca gatcacccag ttcagaaatt
     2521 aatccaaccg cagtaataaa ctgaatccct cgcatggctt gctgcgcctc tactaccgga
     2581 tacagcctcc attcggtaac ggcctccttc gggcggattc cagccgttca cattgtgcct
     2641 gccggtcttc tattgtacaa cggtgttcgt caaaagctaa ttgtcgccag ggactttcga
     2701 atgagtattt gctgagccag tgtcggtgtg cagggcccca atctgctcgc ccgacataat
     2761 ggaccccatg aaccagaaga aacgatttca gacgttgcct tgcatgccgt aaatcatcgc
     2821 gggcagatgc ccatgcccgg gccaaatccc ggaatgcctc atcttcaacg ccggatacgt
     2881 agacagcaga aagatctcct gcacgcagtg aacgcacaag tctgatggca tcacggtgtc
     2941 ggttttaccc cgctcccccg gttttttcgg gataagtgag ggggcgcaga ccatgcagtc
     3001 aaaaccggat ttcaccagcc gacgataaag tccataacca cagggtcccg ctttataaac
     3061 gatactgacc tgcgatgact ttgacctgat acgttgataa agattatgaa tgtcagtagg
     3121 tgatgtgcta attttaccca tcagttcaac aggttctgaa ttgatagcat aggagacagt
     3181 aatcgattct ttgtgaacat ctaattcgac gtacaacatg ttattgttgt tcatgctggc
     3241 ctccgtggtg gaaattccag gggggtagca acagctcacc catgcggctc tgacaacgat
     3301 gttaacccgc attaaatatc ccacgttgga cagcatctgt ctcacggaaa gtcatactgc
     3361 cttacgtgca tatttcacct tacgccttca ctgtctgtca tcggaagtac tgatttatat
     3421 tggggctttg cacttactgt cattcacggg aaaagatggt ggtgaacagt acgatactgt
     3481 cttggctgtt gccgagggaa gattcaggag tggtaagggg gttagtggtg taagatgagt
     3541 ctggagctct gactggtcag tgacgcgaaa ctcaatctgc caggataaac tcccccttgc
     3601 ttttgcgcag aagcagtggg ccttggttcg cttatgtcct gcatctacaa gcgaatgact
     3661 gaccggagac tcccatatct ggtcagtcgc ccatttttat tttccaccac atcggacaag
     3721 cataccgtaa taaacagcac cagtctcctg ttcactcttg cttgatttac tggaatgctg
     3781 tttatggaga tatccggttg aaagatttta cctcttcgac aggactataa attatacagt
     3841 aaggagtccc tcgtagaaag gggctgattc tcatcaagtc ggacaggttc aaagtgttac
     3901 ctgaatgagc taaatgttat ttaccttcct ttctgttcat tgtaaaattt ttcttgctaa
     3961 cgcaaatcat tatcattacg attttagaaa agggaaattt atgaagaaca aatatatcat
     4021 tgctccg
//
LOCUS       DAZFLD010000291         4023 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.293, whole genome shotgun sequence.
ACCESSION   DAZFLD010000291 DAZFLD010000000
VERSION     DAZFLD010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4023
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.293"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1131
                     /gene="pilO2"
                     /locus_tag="ACHKBL_004548"
     CDS             <1..1131
                     /gene="pilO2"
                     /locus_tag="ACHKBL_004548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001681793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4b pilus protein PilO2"
                     /protein_id="HFO5821296.1"
                     /translation="SNEDADTHTMVATVSRRILPGKPRQCFSLALLILPLLEHGGYAI
                     TELTLPGETPLYSFVSVVDGVLVSDLVGSGEEVREARDTFLSINTEPTQGWIHYEPAA
                     FSAGAQNQHLPLASLTGSGKHPAAARLNPVSRGPQFITVSLIIALLGTAWYGWQYYER
                     WQTEKAAQAAVARQATEERITPPWPGLPETDAFIRGCADIWTSLPLSAAGWRYSLAEC
                     STDGGSGNLRASYTNTAGATVTDFIRRISDMTDSRPFIVMPEGKTGGIGLPVTFRLSE
                     NAVTVDALPETAVLQERLTSLAQSMQLKLDWQEVNNSLTDENGQIIQPPWKEYDLQMQ
                     TTLPAHHLAERLKEPSVRFISVTRQLENGRFHYQFTGKYYAR"
     gene            1121..1573
                     /gene="pilP"
                     /locus_tag="ACHKBL_004549"
     CDS             1121..1573
                     /gene="pilP"
                     /locus_tag="ACHKBL_004549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis protein PilP"
                     /protein_id="HFO5821297.1"
                     /translation="MPDKAKTLLLSALLLPATAMATTGNAEQQNTQQIRIEQIQEETA
                     LYEAMLAREKIRHELRTLEQGVPAEGGRQQESISAVTTNAQALPSPRLTAIFGVQPQL
                     TARIELPGGSISDVKAGQNIPGTTYHVEKITPDSVVLEQSGTRKTLRP"
     gene            1658..3166
                     /locus_tag="ACHKBL_004550"
     CDS             1658..3166
                     /locus_tag="ACHKBL_004550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HFO5821298.1"
                     /translation="MLDPADLPPDDILDYVAIDTDNTGQLRIRVVEGKKHLRAVQEYL
                     TRLRARYQGRVGDFEFTTLDVIARLRQDATTTGDENAINPIQQKMLGYIRHSASLGAS
                     DLHMTPGRDNTDFTYVEARVHGELEVLDILRKEEGLELLGATYSGMTDVIKGTQFDPG
                     VPQDARLSEQYLKLAGLFGARYSHYPCVGGLYAVLRLIKDDSQQIPTFSMLGYHPDQE
                     CTLRRILQRPEGIITLSGPTGSGKSTTLRTASAAYLEQYGFNNTGGILLPRRRLFTIE
                     SPPEGRIPGAIQTAVMDTTRGWVDSIKSALRLDPDSILNGEIRDHDSAITAIKAAMTG
                     HLMLTTIHANDPINILERLEMEGVQARMIADPQLFIGLLSQRLVQLICPHCRHPWNDV
                     ASRRTEDERHLVETFCNADAVYLRNHEGCPHCWRGVTGRTVIAEVISPDARFFQLYRE
                     KGRIEAKTYWHRELGGMTRNQHLLGKINSGLVDPLAAHYISPIDEDSYTLLR"
     gene            3168..>4023
                     /locus_tag="ACHKBL_004551"
     CDS             3168..>4023
                     /locus_tag="ACHKBL_004551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000670135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HFO5821299.1"
                     /translation="MKFLEKLNSTELDLSALNSWTARKTFGLKRAEFYESLGGMIRDG
                     TPSLRALEFICNVETDFGTRKGTSGMYFLARECIASLKASGMLSGALPTWVPPEEAAL
                     IRNGEERGNIAEALFQVAKTVKARREMVSSLITLCLYPLLLLSLCVVNMYNAHTRIIP
                     IVSAFAPEEKWSFQMKVLAAMSELVIGYGLWLSGAIITLVIIIRFSLSRYTGPGRRLL
                     DKLPPWSLYQTFHGVNYLFSISSMLQINIPLSQALLRIEKSAEHNKWLKERIQAIRKN
                     VLSGQNLAT"
BASE COUNT          996 a         1139 c         1037 g          851 t
ORIGIN      
        1 agtaatgaag atgccgatac ccacaccatg gtagccactg tcagtcgccg catactgccc
       61 ggaaaacccc gtcagtgctt ctccctcgcc ctgctgatcc tgccactact tgaacatggc
      121 ggatatgcca tcacggaact gacgttaccc ggagaaacac cgctctatag ctttgtttcc
      181 gttgtcgacg gtgttctcgt cagtgacctg gtgggaagcg gggaagaggt tcgggaagcc
      241 cgggatacct ttctttccat caacaccgaa ccgactcagg gctggataca ctatgaacca
      301 gcggcatttt cagcaggcgc tcagaatcag cacctgccgc tggcctccct gactggctct
      361 ggtaaacatc cggctgccgc acgcctgaat ccggtatccc ggggaccgca gttcatcact
      421 gtctctctga tcatcgccct tctcggcact gcatggtacg gctggcagta ttatgaacgc
      481 tggcagacag aaaaagccgc gcaggcagcg gttgcccgac aggccacaga ggaacgaatc
      541 actcccccct ggcccggact ccctgaaaca gacgcgttca tccggggatg tgctgatata
      601 tggacttcac tgccactgtc tgctgccgga tggcgttatt cgctggcaga atgctcgact
      661 gacggtggca gcggcaatct tcgcgcatcg tacaccaata ctgcgggcgc gacagtgact
      721 gacttcatcc ggcgcatcag tgatatgaca gacagccgac cgtttattgt gatgccggaa
      781 ggaaagaccg gcggcattgg cctgccggtc accttccggc tgtctgaaaa cgccgtcaca
      841 gttgatgcac taccggaaac cgctgtcctg caggaacgac tgacctcgct ggctcagtca
      901 atgcaactga aactggactg gcaggaagtt aataacagcc tcactgatga gaacggacag
      961 atcattcagc ccccatggaa agagtatgac cttcagatgc agaccacact ccctgcacac
     1021 catctggctg aacgcctgaa agagccttct gtccgtttta tctctgtcac acggcaactg
     1081 gagaacggtc gtttccacta tcaatttaca ggtaaatatt atgcccgata aagccaaaac
     1141 ccttctgctt tcagccctgc tgttaccggc aacggcaatg gccacaaccg gaaatgcaga
     1201 gcaacagaac acacagcaaa taaggattga acagatacag gaagagacag ccctgtatga
     1261 agccatgctg gcgagagaga agatccgcca tgaactgcga acacttgagc agggcgttcc
     1321 cgcagagggc ggacgtcagc aggaaagcat ttccgcagtt acaacaaatg cacaggcatt
     1381 accatccccc cgcctgacag caatattcgg tgttcagcct caactgactg ccaggataga
     1441 acttccgggg ggaagtatct ccgatgtgaa ggcgggccag aacatccccg gaaccactta
     1501 tcacgtggaa aaaatcacgc cggacagtgt agtgctggaa cagtctggca ccagaaaaac
     1561 cctgcgacca tgagactggc cgccgtaact ggcggctgtc aaaaataaaa ggatgaaaga
     1621 catattttcc gggcaccgct gcccgtaagg aggctttatg ctggatccag cagatctgcc
     1681 gccggatgat attctggatt atgtggccat cgacaccgac aacaccggac aactgcggat
     1741 cagagttgtg gaggggaaaa aacatctgcg tgccgtccag gaatatctga cccgccttcg
     1801 cgcacgctat caggggcgtg tgggagactt tgagttcacc acgctggatg tgatagcccg
     1861 cctcagacag gacgccacaa cgacaggcga tgaaaacgct attaacccga tacagcagaa
     1921 aatgctcggc tatatccgcc attctgcctc ccttggtgcc tccgaccttc atatgacgcc
     1981 agggcgggac aacacagact tcacctatgt ggaagcccgg gttcatggcg aactggaagt
     2041 gctcgatatc ctccggaaag aagaaggtct ggaattactg ggggctacct attccggcat
     2101 gacggatgtc atcaagggca cacaatttga tcccggggtt ccacaggacg cacgtctctc
     2161 tgaacagtac ctgaaactgg ctggcctgtt tggcgcacgc tactcccact acccctgcgt
     2221 gggaggcctc tatgctgttc tccgtctgat caaggatgac agccagcaaa tcccgacctt
     2281 cagtatgctg ggttatcacc cggatcagga gtgcaccctg cgccgtatcc tgcaacgccc
     2341 tgaaggcatt atcaccctct ctggccccac cggctcggga aaatccacca cgctgagaac
     2401 cgcctctgcg gcataccttg aacaatacgg cttcaacaac accggcggta ttcttctgcc
     2461 acggcgcagg ttgttcacga ttgaatcccc gccggaaggc cgtatccccg gagcaattca
     2521 gacagccgtt atggatacca cccggggctg ggtggactcc atcaaatcag cactgcgtct
     2581 tgatcctgac agcatcctga acggtgaaat ccgcgatcac gattccgcca ttacggcaat
     2641 aaaagccgcc atgaccgggc atctgatgct gaccaccatc catgcgaacg acccgattaa
     2701 catcctcgaa cgccttgaga tggagggtgt tcaggccagg atgattgcag acccgcagtt
     2761 gttcatcgga ctgctcagtc agcgtctggt tcagctgata tgtccccact gtcgtcatcc
     2821 atggaatgac gtagcatccc gtcgtactga ggatgagcga catctggtgg aaacattctg
     2881 taatgctgat gcggtatatc tgagaaacca tgaaggctgt ccacactgct ggcgtggtgt
     2941 cacagggcgt acagtgattg cagaagtcat ctctcctgac gccagatttt ttcagttata
     3001 ccgggaaaaa ggccgtatcg aagctaaaac ctactggcac agggaactgg gcgggatgac
     3061 gcgtaatcag catctgctgg gaaaaatcaa cagcggactg gttgaccctc tggcggccca
     3121 ttacatctct cccattgatg aggacagcta tacgctgctg cgctgatatg aaatttctgg
     3181 aaaaactcaa cagtactgaa ctggatctgt ctgccctgaa cagctggaca gccagaaaaa
     3241 ccttcggtct taagcgtgcc gagttctatg agtccctcgg cggaatgata cgtgacggaa
     3301 cgccgtcact gcgcgccctg gagtttatct gtaatgtcga aactgacttt ggcacccgga
     3361 aaggaacatc cggcatgtac tttctggcca gagaatgtat tgcctccctg aaagccagcg
     3421 gtatgctgtc gggggccctg cccacatggg ttcccccgga agaagcggca ctgatcagga
     3481 acggtgaaga acgcgggaac atcgccgaag ccctgttcca ggtcgcaaaa accgtgaaag
     3541 cacggcggga aatggtgagc agtctcatca cactctgtct ttatcccctt ctgctgcttt
     3601 cactctgtgt ggtgaatatg tacaacgcac atacccgcat catccccatt gtcagcgctt
     3661 ttgcaccgga ggagaaatgg tcattccaga tgaaagtcct tgcagccatg tcagagcttg
     3721 tcatcggcta tggtctgtgg ctcagtggtg ccatcatcac gcttgtcatc atcatccgct
     3781 tctcactcag tcgctatacc ggcccgggac gcaggcttct tgacaaactg ccaccatggt
     3841 cgctctatca gacttttcat ggtgtgaatt atctgttcag catatcgtca atgctgcaga
     3901 taaatattcc gctttcacag gcgctgctca gaattgaaaa aagtgcagag cataataaat
     3961 ggttaaagga gagaattcag gcaataagaa aaaatgtatt atccggccag aatctggcca
     4021 ccg
//
LOCUS       DAZFLD010000292         4022 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.294, whole genome shotgun sequence.
ACCESSION   DAZFLD010000292 DAZFLD010000000
VERSION     DAZFLD010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4022)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4022
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.294"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..634)
                     /locus_tag="ACHKBL_004552"
     CDS             complement(<1..634)
                     /locus_tag="ACHKBL_004552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000619857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="HFO5821300.1"
                     /translation="MIRKSCIAACLSITLCSLSYAGGVPTVSIAELTQLVVNAQQQAQ
                     EALAQLNKAKEAIRQAKDQYDHYKSILQGNDKLGAFLNDPYINKLLPMKDWQDIYNQT
                     QDLTDLRKRYGITGYEPDVQKLFDKLLAQVDVYEKQYDATVTRVNHAEELRNKLNSVQ
                     TPKDREQLALRYQQEMLELENQRMQLENTRYLMEQKERIENKKNEEDFYNF"
     gene            complement(704..3238)
                     /locus_tag="ACHKBL_004553"
     CDS             complement(704..3238)
                     /locus_tag="ACHKBL_004553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB4 family type IV secretion system protein"
                     /protein_id="HFO5821301.1"
                     /translation="MQDALFKNAFSPESNAKVPWLGHHVAPEICTLGTSHILSLIRFS
                     GVPHDTRDQAMLNKEFMYLARCFKALGKQEGNNLKLQTYTFKRRIHLTHQYNLELPVL
                     QDLVDCWVKPFRNGTFRQVGYSMALILKFRDLDDGINRMRELLSLCREMLGDFGVSVM
                     ELEERDGKLYSQIGRFYSFILNGEEKDIPVTDTRLGDSIIDSETGFGAYDYVENRPSY
                     GKRRFASTYDLRDYPESKSYPGMWDEVLDEQYDFCMVQSFLFEDRNRMKRRLKTQMSD
                     LSSTEGQSSETEELADSVQEVTQGKKVFGEYYASLIVFGDTPEEAVDNGARMQSLLMA
                     RDTGFVRSRSTNYVTWLTLFPGYADVIYSMPKSTENLACGFSLHATPCGKADGNPPGD
                     GTALMPVMTENGGMYFLNAHASPLGKNCRGEKYPGHKTTIAMTGAGKTTVESLELVFF
                     SRWNPMIFSLDYNNSLENVLRALGTRYFNIKPGEFTWIQPFQMEDSVELRQYLFDVLV
                     QCAGGADSTEESKIQMSIDAVLKHKNVESRSFSLLLQSIPDTGPGGLRERLSKWCRDD
                     GNGRRGQYYWILDSPRNDFDPTLFRRLGFDCTKILNMEFATRHPQAMEVLLNTFFFLK
                     RRMHENEPGSLLINSIAEFWAPLMFESTANSIMEILHAGRTRGEMVMMDTQVPEYVLN
                     NERGPSVIQQTITQEWMANEKATSDSYSKFSVTSKEFSKIEEFSAYSRKVLVKQGGAS
                     VVLKVDMQDKLKYWLPLLSSDYANIPVAKRVREALGTDDPAVWVAPFLDEMVAIGVRK
                     ELAEKYPEQDVNDPQVWEPAFIEVMKQLERPVSMKLTIINNINSKS"
     gene            complement(3195..3566)
                     /locus_tag="ACHKBL_004554"
     CDS             complement(3195..3566)
                     /locus_tag="ACHKBL_004554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000504825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB3 family type IV secretion system protein"
                     /protein_id="HFO5821302.1"
                     /translation="MGETNEILEFDTNNAMNRVAMFAGIPVFALLFLVVIGCILTFSS
                     VWMWGWWGFIVTIPFILAIVGLRIICERDDKAFRRFRFQLRRWKMNRKYGRHLLLTSR
                     NPRWRDKYARRVIQKRILTGK"
     gene            complement(3566..3907)
                     /locus_tag="ACHKBL_004555"
     CDS             complement(3566..3907)
                     /locus_tag="ACHKBL_004555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000659014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5821303.1"
                     /translation="MKEIMKKMIGLFSIPSRYIALLFVFIPVYAHAAWYDPIVKFVKE
                     FQTGIIIVGGAVAVGSLVYVGVCWIISRMSGNMETTAMDYVKHIGVIGAVGGAVAAAT
                     WAYQLWGGTIS"
BASE COUNT         1204 a          969 c          660 g         1189 t
ORIGIN      
        1 taaaattata aaaatcctcc tcattctttt tattttcaat tcgttctttt tgctccataa
       61 gatagcgcgt attttccagt tgcattctct gattttcaag ctctaacatt tcctgttgat
      121 atcttaatgc taactgctcc ctatcctttg gagtctgaac tgaatttagt ttatttctca
      181 actcttcagc atggtttact cgtgtgactg ttgcatcata ttgtttttca tatacatcaa
      241 cttgagcaag taacttatca aacaattttt gaacatcagg ttcatacccg gtaataccat
      301 aacgctttct taaatcagtt aagtcttggg tttgattata tatatcctgc caatctttca
      361 ttggcagtaa tttatttata tatggatcat tcagaaatgc ccccaattta tcgtttccct
      421 gaagaatcga tttataatga tcgtactgat cttttgcctg tcgaattgcc tcctttgctt
      481 tattaagctg cgccaacgct tcctgcgcct gttgctgtgc atttacaact aattgcgtta
      541 actctgcaat gcttaccgtt ggcactccac ctgcataact tagagaacat aacgttatag
      601 agagacacgc ggctatacat gatttacgta tcatatagaa ctcctgtgtt tatcaggaat
      661 gaacgttttc gttaaacatc aaagcattcc tgatatcata aaatcaagac tttgagttta
      721 tattattgat gattgtcaac ttcatagaaa ctggacgttc cagttgcttc atcacttcaa
      781 taaatgcagg ctcccatact tgaggatcat tgacatcttg ttcaggatac ttttccgcaa
      841 gctctttcct tactccaata gccaccatct catccagaaa tggggccacc catacagccg
      901 gatcatctgt acctaacgct tccctaactc gtttagctac aggtatattg gcatagtcag
      961 atgacaataa aggcaaccaa tatttcagct tatcctgcat atccaccttc agaacgacag
     1021 atgcacctcc ctgttttacc agaaccttac gtgaataagc cgaaaactcc tctattttac
     1081 tgaactcctt agaagttaca gaaaatttac tgtaactgtc tgaggtagct ttttcatttg
     1141 ccatccattc ctgcgtaatt gtctgctgaa taactgaagg accacgctcg ttattcagaa
     1201 catattcagg aacctgagta tccatcataa ccatctcacc acgggtcctc cctgcatgta
     1261 aaatttccat aatggaatta gctgtactct caaacattag aggtgcccaa aattccgcta
     1321 tactgtttat tagtaagctg cctggttcat tctcatgcat tcttctcttc aggaaaaaga
     1381 aggtatttaa taatacttcc attgcctgag gatgacgtgt agcaaactcc atgttaagaa
     1441 tttttgtaca atcaaatcca agacggcgaa acaacgtagg gtcgaaatca tttctgggac
     1501 tgtccaagat ccagtaatac tgtccacgtc ttccattccc atcatcacga caccactttg
     1561 ataatcgttc cctgagtccg ccaggaccgg tatcaggtat agactgtaat aacagtgaaa
     1621 aactacgact ttctacattt ttatgcttca gaacagcatc aatcgacatc tgtattttag
     1681 attcttcagt gctgtcagca cctccggcac actgaacaag aacatcaaac agatattgac
     1741 gtagttcaac cgaatcttcc atttgaaacg gttgtatcca ggtaaattca ccaggtttta
     1801 tattaaaata acgggttcca agagcacgga gtacgttctc cagcgaatta ttgtaatcca
     1861 gagaaaatat cataggattc cagcgactaa aaaacactaa ttcaaggctc tcaaccgttg
     1921 ttttacctgc cccagtcata gcaattgtcg ttttatgccc tggatacttc tcacctctac
     1981 agttcttccc taaaggactg gcgtgtgcat tcagaaaata cattcccccg ttttccgtca
     2041 tcactggcat caacgctgtt ccgtctccag gaggattccc atcggctttc ccacacggag
     2101 tcgcgtgtag ggaaaatcca caggccaagt tttctgtaga ttttggcatc gaataaatca
     2161 catccgcata accaggaaat agcgtcagcc aggttacata atttgtactt cgggaacgaa
     2221 caaagccggt atcgcgcgcc attaacaacg attgcattct tgctccgtta tcaacagctt
     2281 cttcaggtgt atctccaaat acaatcaggc tcgcataata ttcaccaaaa acttttttcc
     2341 cctgggtaac ttcctgaaca ctatcagcaa gttcttcagt ttcactggat tgtccttccg
     2401 tagacgataa atccgacatc tgagttttca ggcgacgttt cattcggtta cgatcttcaa
     2461 aaagaaaaga ttgaaccata caaaagtcat actgttcatc cagcacttca tcccacattc
     2521 ctgggtagga tttgctttct ggataatccc ttagatcata agtagaagca aatcggcgct
     2581 ttccatacga cgggcggttt tccacataat cgtaagcgcc aaatcctgtt tcactgtcaa
     2641 taatcgagtc tcccaggcgg gtgtcagtaa caggaatatc tttctcttca ccgttcagaa
     2701 taaacgagta aaagcggcca atctgcgaat acagttttcc gtcccgctcc tccagctcca
     2761 taacagaaac accaaaatcc cccagcatct ctctacataa agataacaat tcccgcattc
     2821 ggttaatacc atcgtccaga tcccgaaact taaggataag ggccatagaa tatccaactt
     2881 gccggaatgt cccattacga aatggtttta cccagcaatc aaccaaatcc tgaagaacag
     2941 gtaattcaag attatactga tgtgtgagat gtatacgacg cttaaaagtg tacgtctgca
     3001 atttcaggtt attcccttcc tgttttccca aagctttaaa acatctggca agatacataa
     3061 actctttatt tagcatagcc tgatctcgtg tatcatgagg aacaccagaa aaacggatca
     3121 aactgagaat atgagacgtg cccagagtac aaatttcagg tgcaacatgg tgccccaacc
     3181 agggcacctt agcattactt tccggtgaga atgcgttttt gaataacgcg tcttgcatat
     3241 ttatctctcc atcggggatt tctggaagtc aacagtaagt gacgaccata tttacgattc
     3301 attttccagc ggcgtaattg aaaacgaaaa cgacgaaacg ctttgtcatc acgttcacaa
     3361 ataatacgca atcctacgat tgccagaata aaagggatgg tgacaataaa tccccaccat
     3421 ccccacatcc atacagaaga aaatgtcaaa atacagccaa taacaaccaa aaaaagaagt
     3481 gcaaaaacag ggatccctgc aaacatagcc accctgttca tcgcgttatt agtgtcaaac
     3541 tcgagaattt cgtttgtctc tcccatcagc taattgttcc tccccacaac tggtacgccc
     3601 aggtagctgc ggcaacagcg ccaccaacag caccaataac accaatatgt ttcacataat
     3661 ccattgccgt tgtttccata ttcccggaca tacgagaaat aatccaacag actcccacgt
     3721 agacaagaga accaacagca acagcgcccc cgacaataat aattcctgtc tgaaactcct
     3781 taacaaattt aactattgga tcataccatg ctgcatgagc atatacagga ataaaaacaa
     3841 ataataacgc aatataacga gatggtatac taaacagtcc tatcatcttt ttcattattt
     3901 ctttcattaa ttaaaacctc tttttttgat ccatatccat ttaattgttt tggatattcc
     3961 ctgagaatat cccatgattc aaaaataata tcatcatgag ttttatcttt tatgtcatta
     4021 at
//
LOCUS       DAZFLD010000293         4007 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.295, whole genome shotgun sequence.
ACCESSION   DAZFLD010000293 DAZFLD010000000
VERSION     DAZFLD010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4007
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.295"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(99..1778)
                     /gene="bcsG"
                     /locus_tag="ACHKBL_004556"
     CDS             complement(99..1778)
                     /gene="bcsG"
                     /locus_tag="ACHKBL_004556"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HFO5821304.1"
                     /translation="MTQFTQNTAMPSSLWQYWRGLSGWNFYFLVKFGLLWAGYLNFHP
                     LLNLVFAAFLLMPIPRYSLHRLRHWIALPIGFALFWHDTWLPGPESIMSQGSQVAGFS
                     TDYLIDLVTRFINWQMIGAIFVLFVAWLFLSQWIRITVFVVAILLWLNVLTLAGPSFS
                     LWPAGQPTTTVTTTGGNAAATVAATGGAPVVGDMPAQTAPPTTANLNAWLNNFYNAEA
                     KRKSTFPSSLPADAQPFELLVINICSLSWSDIEAAGLMSHPLWSHFDIEFKNFNSATS
                     YSGPAAIRLLRASCGQTSHTNLYQPANNDCYLFDNLSKLGFTQHLMMGHNGQFGGFLK
                     EVRENGGMQTELMDQTNLPVILLGFDGSPVYDDTAVLNRWLDITEKDKNSRSATFYNT
                     LPLHDGNHYPGVSKTADYKARAQKFFDELDAFFTELEKSGRKVMVVVVPEHGGALKGD
                     RMQVSGLRDIPSPSITDVPVGVKFFGMKAPHQGAPIVIDQPSSFLAISDLVVRVLDGK
                     IFSEDNVDWKKLTSGLPQTAPVSENSNAVVIQYQDKPYVRLNGGDWVPYPQ"
     gene            complement(1775..1966)
                     /gene="bcsF"
                     /locus_tag="ACHKBL_004557"
     CDS             complement(1775..1966)
                     /gene="bcsF"
                     /locus_tag="ACHKBL_004557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0052324 - plant-type cell wall cellulose
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsF"
                     /protein_id="HFO5821305.1"
                     /translation="MMTISDIIEIIVVCALIFFPLGYLARHSLRRIRDTLRLFFAKPR
                     YVKPAGTLRRTEKARATKK"
     gene            complement(1963..3534)
                     /gene="bcsE"
                     /locus_tag="ACHKBL_004558"
     CDS             complement(1963..3534)
                     /gene="bcsE"
                     /locus_tag="ACHKBL_004558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis c-di-GMP-binding protein
                     BcsE"
                     /protein_id="HFO5821306.1"
                     /translation="MRDIVDPVFSIGISSLWDELRHMPAGGVWWFNVDRHEDAISLAN
                     QTIASQAETAHVAVISMDSDPAKIFQLDDSQGPGKITLFSMLNHEKGLYYLARDLQCS
                     IDPHNYLFILVCANNAWQNIPAERLRSWLDKMNKWSRLNHCSLLVINSGNNNDKQFSL
                     LLEEYRSLFGLASLRFQGDQHLLDIAFWCNEKGVSARQQLSVQQQNGCWTLVQHQEAE
                     IQPRSDEKRILSNVAVLEGAPPLSEHWQLFNNNEVLFNEARTAQAATVVFSLQQNAQI
                     EPLARSIHTLRRQRGSAMKILVRENTASLRATDERLLLACGANMVIPWNAPLSRCLTM
                     IESVQGQKFSRYVPEDITTLLSMTQPLKLRGFQKWDVFCNAVNNMMNNPLLPAHGKGV
                     LVALRPVPGIRVEQALTLCRPNRTGDIMTIGGNRLVLFLSFCRINDLDTALNHIFPLP
                     TGDIFSNRMVWFEDDQISAELVQMRLLAPEQWGMPLPLTQSSKPVINAEHDGRHWRRI
                     PEPMRLLDDAVERSS"
     gene            3807..3995
                     /gene="bcsR"
                     /locus_tag="ACHKBL_004559"
     CDS             3807..3995
                     /gene="bcsR"
                     /locus_tag="ACHKBL_004559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsR"
                     /protein_id="HFO5821307.1"
                     /translation="MNNNEPDTLPDPAIGYIFQNDILALKQAFSLPDIDYADISQREQ
                     LAAALKRWPLLAEFAQQK"
BASE COUNT         1050 a          977 c         1013 g          967 t
ORIGIN      
        1 aagcggaagt aacgtgtcat gcgggcgtca ggctgccgta atggcaattt gcgcccggat
       61 caggccgcag gggtgaaact ctgcggcctt tttcgttctt attgcgggta aggcacccag
      121 tcgccgccgt tcaggcgaac gtacggttta tcctggtatt gaataactac tgcatttgag
      181 ttctcggaga ccggtgctgt ttgtggcaac ccactggtga gttttttcca gtcaacattg
      241 tcttcggaga aaatcttgcc atcaagaacg cgaaccacca gatcggagat agccaggaag
      301 ctgctcggtt ggtcgatgac aatcggtgcc ccctgatgtg gtgccttcat gccgaagaat
      361 ttcaccccaa cggggacgtc tgtgatagac gggctaggga tatcacgtag gccagatacc
      421 tgcattctgt cgcccttcag cgcgccgccg tgttccggca ccacgaccac catcacttta
      481 cgacccgatt tctccagttc agtaaagaag gcgtccagtt catcaaagaa tttctgcgcc
      541 cgcgctttgt aatccgctgt tttgctgacg cccggataat ggttgccgtc atgcagtgga
      601 agcgtgttgt agaacgtggc actacggctg tttttatctt tttcggtaat gtccagccag
      661 cggttaagca cggcggtatc gtcataaacc ggcgaaccat caaagcccag caaaataacc
      721 ggcagatttg tttgatccat caattcggtc tgcatgccgc cattttcgcg aacttctttc
      781 aaaaaaccgc cgaactggcc gttatgtccc atcatcaggt gctgggtaaa gcccagtttc
      841 gacaggttat caaacagata gcagtcgtta tttgccggtt gatacagatt agtgtgcgaa
      901 gtctgcccgc agctggcgcg cagtaaacgg atcgccgccg ggccactgta ggaggtggcg
      961 gagttaaagt tcttgaactc aatatcgaaa tgcgaccaca gcggatgcga catcaacccg
     1021 gcggcttcaa tatccgacca ggaaagcgaa cagatattaa tcaccagtag ttcaaatggc
     1081 tgagcatcag cgggcagcga agacgggaag gtcgatttac gtttcgcctc cgcgttatag
     1141 aaattattta gccaggcgtt aaggttcgcc gttgttggcg gtgcagtttg tgcgggcata
     1201 tcacccacta ccggtgcgcc acccgtcgcc gcaacggttg ccgctgcgtt accacccgtc
     1261 gttgttacag tggtcgtcgg ttgaccggct ggccacaagg agaaacttgg tcccgccagg
     1321 gtaagtacgt tcagccatag cagaatggca accacaaaaa cggtaatgcg aatccattgt
     1381 gacaggaata accaggccac gaataaaaca aaaatggccc caatcatctg ccagttaata
     1441 aagcgtgtga caaggtcgat taaataatcg gtactgaacc ccgccacctg cgaaccctgg
     1501 ctcattatgc tttccgggcc aggcaaccag gtgtcatgcc agaacaaagc aaagccgatc
     1561 ggcagggcaa tccagtggcg caagcgatgc aggctgtagc gcggaatggg catcagcaga
     1621 aacgcggcaa agaccaaatt gaggagcgga tggaagttaa gatatcccgc ccacaacagg
     1681 ccgaacttaa ccagaaaata gaagttccag ccggaaaggc cgcgccagta ttgccagagg
     1741 gaagaaggca tggcggtatt ttgcgtaaat tgagtcattt tttggttgcc ctggcttttt
     1801 ccgtgcggcg taacgtcccg gccggtttaa cataacgagg tttagcaaag aacaaacgta
     1861 aggtgtcgcg aatgcgtcgc aaagagtgcc gcgccagata gcccagcggg aaaaatatca
     1921 gtgcgcaaac gacaataatt tcaatgatat cgctgatggt catcatgatg agcgctccac
     1981 agcatcatct aacagtcgca ttggttctgg tattcgtcgc cagtggcgac catcgtgctc
     2041 ggcattgatg accggtttag aactttgcgt taaaggcagc ggcatgcccc attgttctgg
     2101 ggcaagcagg cgcatctgca ccagctcggc actgatttga tcatcttcaa accagaccat
     2161 acggtttgag aaaatgtcgc cagtaggcaa tgggaaaata tgattcaacg cggtatccag
     2221 atcgttaatc cgacagaatg agagaaacag caccagccga ttaccgccaa tggtcatgat
     2281 atcgccggtg cgattagggc gacatagcgt cagggcttgc tcaacgcgga tacccggtac
     2341 cggacgtagg gcaaccagaa cgcctttacc gtgggcaggt aatagagggt tattcatcat
     2401 gttgttgacg gcattacaga acacatccca cttctggaaa ccacgcagtt tgagcggctg
     2461 ggtcattgac agcaaggtag tgatatcttc cggcacatag cgactaaact tctgcccttg
     2521 cacgctttcg atcatcgtca gacagcggga gagtggcgca ttccacggga taaccatatt
     2581 tgcaccgcag gccaataata aacgttcatc ggtggcgcgc aggctagcgg tattttcccg
     2641 tacgaggatt ttcatcgcac taccgcgctg gcgacgcaga gtatgaatgc tgcgggccag
     2701 tggctcgatt tgcgcatttt gctgtaaaga aaagaccacc gtcgccgcct gagcggtacg
     2761 ggcttcattg aacaggactt cgttattgtt gaacagttgc cagtgttccg atagcggcgg
     2821 cgcaccttcc agtacagcaa cattactcag aatgcgtttt tcgtcgctgc gtggttggat
     2881 ttccgcctcc tggtgttgaa ctaatgtcca gcaaccattt tgttgctgaa cgctaagctg
     2941 ctgacgggcg ctgacccctt tttcgttgca ccagaaagca atatccagca aatgttggtc
     3001 gccctgaaaa cgcaaactgg caagaccaaa aagtgaacgg tattcctcaa gtaacaatga
     3061 aaattgttta tcgttattat ttccggaatt aattaccaaa agcgaacaat ggtttagccg
     3121 gctccattta ttcattttat ccaaccatga gcgaagccgc tcggcaggaa tgttttgcca
     3181 tgcgttattt gcgcaaacaa gaataaaaag gtaattatgg ggatcaatag aacactgcaa
     3241 atcacgggcc aaatagtata gacctttttc atgatttagc attgaaaata atgttatttt
     3301 tcccggccct tgagaatcat ctaattgaaa gattttcgct ggatcgctgt ccatgctaat
     3361 gaccgcgacg tgtgcggttt cagcctggga tgcaattgtt tgattcgcca gactgatagc
     3421 atcttcatgg cgatcgacgt taaaccacca gacgccgcct gctggcatat gtcgcagctc
     3481 atcccataat gatgagatac cgatagagaa tacagggtcc acaatgtccc tcatgtcaac
     3541 attttgtctg attatcagtt tactagcgaa agctaagaaa taaacctacc attgaaatta
     3601 aaacttatca gatttagcat gtgaatcaat ttagcaggtc acaagacatg tgattttagt
     3661 cttttcgttt ttaacttttt ctgttgcgta acggaaagtc aaaaagtgag cacattcccg
     3721 tctcgccgta aacaataaac cgacgaaatg ctcacagtta gaattgtctc atgaacggta
     3781 cggttatttc atagggatca agaaaaatga ataacaatga accagatact ctgcctgatc
     3841 ccgcgatagg ctatatcttc cagaatgata ttttggcgtt aaagcaggca ttttcactgc
     3901 ctgatattga ttatgccgat atttcccaac gcgaacagtt ggccgcggca ttaaaacgct
     3961 ggccgttgct ggcagagttt gcgcaacaaa agtaggggat tggtgaa
//
LOCUS       DAZFLD010000294         3963 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.296, whole genome shotgun sequence.
ACCESSION   DAZFLD010000294 DAZFLD010000000
VERSION     DAZFLD010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3963
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.296"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            114..317
                     /gene="aaeX"
                     /locus_tag="ACHKBL_004560"
     CDS             114..317
                     /gene="aaeX"
                     /locus_tag="ACHKBL_004560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump operon protein
                     AaeX"
                     /protein_id="HFO5821308.1"
                     /translation="MSLFPVIVVFGLSFPPIFFELLLSLAIFWLVRRVLVPTGIYDFV
                     WHPALFNTALYCCLFYLISRLFV"
     gene            325..1257
                     /gene="aaeA"
                     /locus_tag="ACHKBL_004561"
     CDS             325..1257
                     /gene="aaeA"
                     /locus_tag="ACHKBL_004561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeA"
                     /protein_id="HFO5821309.1"
                     /translation="MKTLIRKFSRTAITVVLVILAFIAIFNAWVYYTESPWTRDARFS
                     ADVVAIAPDVSGLITQVNVHDNQLVKKGQVLFTIDQPRYQKALEEAQADVAYYQVLAQ
                     EKRQEAGRRNRLGVQAMSREEIDQANNVLQTVLHQLAKAQATRDLAKLDLERTVIRAP
                     ADGWVTNLNVYTGEFITRGSTAVALVKQNSFYVLAYMEETKLEGVRPGYRAEITPLGS
                     NKVLKGTVDSVAAGVTNASSTRDDKGMATIDSNLEWVRLAQRVPVRIRLDNQQENIWP
                     AGTTATVVVTGKQDRDESQDSFFRKMAHRLREFG"
     gene            1263..3230
                     /gene="aaeB"
                     /locus_tag="ACHKBL_004562"
     CDS             1263..3230
                     /gene="aaeB"
                     /locus_tag="ACHKBL_004562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeB"
                     /protein_id="HFO5821310.1"
                     /translation="MGIFSIANQHIRFAVKLATAIVLALFVGFHFQLETPRWAVLTAA
                     IVAAGPAFAAGGEPYSGAIRYRGFLRIIGTFIGCIAGLVIIIAMIRAPLLMILVCCIW
                     AGFCTWISSLVRIENSYAWGLAGYTALIIVITIQPEPLLTPQFAVERCSEIVIGIVCA
                     IMADLLFSPRSIKQEVDRELESLLVAQYQLMQLCIKHGDGEVVDKAWGDLVRRTTALQ
                     GMRSNLNMESSRWARANRRLKAINTLSLTLITQSCETYLIQNTRPELITDTFREFFDT
                     PVETAQDVHKQLKRLRRVIAWTGERETPVTIYSWVAAATRYQLLKRGVISNTKINATE
                     EEILQGEPEVKVESAERHHAMVNFWRTTLSCILGTLFWLWTGWTSGSGAMVMIAVVTS
                     LAMRLPNPRMVAIDFIYGTLAALPLGLLYFLVIIPNTQQSMLLLCISLAVLGFFLGIE
                     VQKRRLGSMGALASTINIIVLDNPMTFHFSQFLDSALGQIVGCVLAFTVILLVRDKSR
                     DRTGRVLLNQFVSAAVSAMTTNVARRKENHLPALYQQLFLLMNKFPGDLPKFRLALTM
                     IIAHQRLRDAPIPVNEDLSAFHRQMRRTADHVISARSDDKRRRYFGQLLEELEIYQEK
                     LRIWQAPPQVTEPVHRLAGMLHKYQHALTDS"
     gene            3322..3594
                     /gene="yhcO"
                     /locus_tag="ACHKBL_004563"
     CDS             3322..3594
                     /gene="yhcO"
                     /locus_tag="ACHKBL_004563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HFO5821311.1"
                     /translation="MNIYTFDFDEIESQEDFYRDFSQAFGLAKDKVRDLDSLWDVLMN
                     DVLPLPLEIEFVHLGEKTRRRFGALILLFDEAEEELEGHLRFNVRH"
     gene            complement(3649..3912)
                     /gene="yhcN"
                     /locus_tag="ACHKBL_004564"
     CDS             complement(3649..3912)
                     /gene="yhcN"
                     /locus_tag="ACHKBL_004564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid stress response protein YhcN"
                     /protein_id="HFO5821312.1"
                     /translation="MKIKTTVTALSVLSVLSFGAFAADSIDAAQAQNREAIGTVSVSG
                     VASSPMDMREMLNKKAEEKGATAYQITEARSGDTWHATAELYK"
BASE COUNT          906 a          962 c         1063 g         1032 t
ORIGIN      
        1 attaaatatc aatctacagc aatgttgctc tcgcccggct ttccatgccc ttctctgcgg
       61 cgacagatgc tgaaaataat aacgcctgct ctctctatac aaccaaggtc aacatgagtc
      121 tgtttcccgt tatcgtggtg tttgggctgt ccttcccacc gatatttttt gaattgcttt
      181 tatcactggc gattttctgg ctggtgcgcc gggtacttgt gccaacaggt atctacgact
      241 ttgtctggca tccggcgttg ttcaacaccg cgctctattg ctgcttgttt tatttgatat
      301 cgcgactgtt cgtttgaggt tgaagtgaaa acactaataa gaaaattctc ccgtacggcc
      361 atcacggtcg tattagtcat tctggccttc atcgcaattt ttaatgcctg ggtctattac
      421 accgaatccc cgtggacgcg tgacgcgcgc tttagcgctg acgtcgttgc gatcgcgccg
      481 gacgtttctg gactcattac ccaggtgaat gttcatgata accagctggt gaaaaaagga
      541 caggtactgt tcaccatcga ccagccgcgc tatcaaaagg cgcttgagga agcgcaagcc
      601 gatgttgctt attatcaggt actggcacag gagaaacgcc aggaggcagg acgtcgtaac
      661 cgtctcggtg tgcaggcgat gtctcgcgaa gagatcgatc aggccaacaa cgtactacaa
      721 acggttctgc atcagttagc gaaagcgcag gcgacccgtg atctggcaaa actggatctt
      781 gaacgtacag tcatccgcgc gccagcagat ggctgggtga ccaacctcaa cgtctatacc
      841 ggtgagttta ttactcgagg atcaacggcg gttgcgctgg tgaaacagaa ctccttctat
      901 gtactggcct atatggaaga aactaagctg gaaggggtgc gccctgggta tcgtgcggag
      961 atcacgccgc ttggcagtaa caaagtgctg aaagggactg ttgatagtgt tgccgcaggg
     1021 gtcaccaacg ccagcagcac gcgtgacgac aaagggatgg cgaccatcga ctctaacctt
     1081 gaatgggtac gtttggcgca acgtgttccg gtacgtattc gtctcgacaa ccagcaagag
     1141 aacatctggc ctgcgggcac cactgctaca gtggtggtca ctggcaaaca agatcgcgac
     1201 gaaagccagg attcgttctt ccgtaaaatg gcccatcgcc tgcgtgagtt tggttaatca
     1261 cgatgggtat tttctccatt gctaatcaac atattcgctt tgcggtaaaa ctggcgaccg
     1321 ccattgtact ggcgctgttt gttggctttc acttccagct ggaaacgcca cgctgggcgg
     1381 tactgacagc ggcgattgtt gccgccggtc cggcctttgc tgcgggaggt gaaccgtatt
     1441 ctggcgctat tcgctatcgt ggctttttgc gcatcatcgg cacatttatt ggctgtattg
     1501 ctggactggt aatcatcatt gcgatgatcc gcgcaccatt attgatgatt ctggtgtgct
     1561 gtatctgggc cggtttttgt acctggatat cctcgctggt acgaatagaa aactcgtatg
     1621 cgtgggggct ggccggttat accgcgctga tcattgtgat caccattcag ccggaaccat
     1681 tgcttacgcc gcagtttgcc gtcgaacgtt gtagcgagat cgttatcggt attgtgtgtg
     1741 cgattatggc ggatttgctc ttttctccgc gatcgatcaa acaagaagtg gatcgagagc
     1801 tggaaagttt gctggtcgcg caatatcaat taatgcaact ctgtatcaaa catggcgatg
     1861 gtgaagttgt cgataaagcc tggggcgatc tggtgcgacg caccacggcg ttacaaggta
     1921 tgcgcagcaa cctgaatatg gaatcttccc gctgggcgcg ggccaatcga cgtttaaaag
     1981 cgatcaatac gctatcgctg acgctgatta cccaatcctg cgaaacttat cttattcaga
     2041 atacgcgccc ggaattgatc actgatactt tccgcgaatt ttttgacacg ccggtagaaa
     2101 ccgcgcagga cgtccacaag cagctcaaac gcctgcggag agttatcgcc tggaccgggg
     2161 aacgggaaac gcctgtcacc atttatagct gggtcgcggc ggcaacgcgt tatcagcttc
     2221 tcaagcgcgg cgttatcagt aacacaaaaa tcaacgctac tgaagaagag atcctgcaag
     2281 gcgaaccgga agtcaaagta gagtcagccg aacgtcatca tgcgatggtt aacttctggc
     2341 gaaccacact ttcctgcatt ctggggacgc ttttctggct gtggacgggc tggacttccg
     2401 gcagtggtgc aatggtgatg attgcggtcg tgacgtcact ggcaatgcgt ttgccgaatc
     2461 cacgcatggt ggcgatcgac tttatctatg ggacgctggc ggcgctgccg ttagggctgc
     2521 tctacttttt ggtgattatc cctaataccc aacaaagcat gttattgctg tgtattagcc
     2581 tggcagtgct gggatttttc ctcggtatag aagtacagaa acggcgactg ggctcgatgg
     2641 gggcgctggc cagcaccata aatattatcg tgctggataa cccgatgact ttccatttca
     2701 gtcagtttct cgacagcgca ttagggcaaa tcgtcggctg tgtgctcgcg ttcaccgtta
     2761 ttttgctggt gcgggataaa tcgcgcgaca ggactggacg tgtactgctt aatcagtttg
     2821 tttctgccgc tgtttccgcg atgactacca atgtggcacg tcgtaaagag aatcacctcc
     2881 cggcacttta tcagcagctg tttttgctga tgaataagtt cccaggggat ttgccgaaat
     2941 ttcgcctggc gctgacgatg attatcgcgc accagcgcct gcgtgatgcg ccgatcccgg
     3001 ttaacgagga tttatcggcg tttcaccgac aaatgcgccg cacagcagac catgtgatat
     3061 ctgcccgtag cgatgataaa cgtcgtcggt actttggcca gttgctggaa gaacttgaaa
     3121 tctaccagga aaagctacgc atctggcaag cgccaccgca ggtgacggaa ccggttcatc
     3181 ggctggcggg gatgctccat aagtatcaac atgcgttgac cgatagttaa gtcaaaaccg
     3241 acgcccgaag cgtcggtttt ttcatggcta tacttagcga tgaacggcag aactcgccgt
     3301 gaaacgtgac ggtggcaaca gatgaatatt tatacctttg attttgatga gattgagagt
     3361 caggaggatt tttatcgcga cttcagccaa gcctttggtc tggcgaaaga taaggtacgc
     3421 gatctcgact cactatggga tgtgttaatg aacgatgtcc tgccgctacc acttgagatt
     3481 gaatttgttc atctgggaga gaaaacgcgt cgccgttttg gcgcgttaat attgctgttt
     3541 gatgaggcag aggaagagct ggaagggcat ttgcgtttta atgttcgtca ttagcgcaaa
     3601 aaaagccccc gaaccggggg caatatcgtc ggacaagacg atgagggttt atttgtacag
     3661 ttcagccgta gcgtgccagg tgtcaccgct acgagcttca gtaatctggt aggccgttgc
     3721 gcctttctct tccgcttttt tgttcagcat ttcacgcata tccattggcg aagacgccac
     3781 accacttacg gatacggtcc cgattgcttc acgattttgt gcttgtgcag catcaatgga
     3841 gtcggcagcg aatgcaccga aagagagaac agaaagtacg cttaatgcag taacagtggt
     3901 tttgattttc atgattttta cctcgacata atcttttagc tgggcctttg tttcgtgacc
     3961 cat
//
LOCUS       DAZFLD010000295         3919 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.297, whole genome shotgun sequence.
ACCESSION   DAZFLD010000295 DAZFLD010000000
VERSION     DAZFLD010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3919)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3919
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.297"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            12..470
                     /gene="asnC"
                     /locus_tag="ACHKBL_004565"
     CDS             12..470
                     /gene="asnC"
                     /locus_tag="ACHKBL_004565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HFO5821313.1"
                     /translation="MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLDEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQNPIMRTIKP"
     gene            560..1003
                     /gene="mioC"
                     /locus_tag="ACHKBL_004566"
     CDS             560..1003
                     /gene="mioC"
                     /locus_tag="ACHKBL_004566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HFO5821314.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEEAGFTTETLHGPLLEDL
                     SASGIWLVISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGAIGIGSREYDTFCGAI
                     DKLEAELKNSGAKQTGETLKINILDHDIPEDPAEEWLGSWINLLK"
     gene            1382..3271
                     /gene="mnmG"
                     /locus_tag="ACHKBL_004567"
     CDS             1382..3271
                     /gene="mnmG"
                     /locus_tag="ACHKBL_004567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HFO5821315.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRV
                     LYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGDNPMPVFSFMGNASQHPQQVPCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLAGLNAA
                     RLSADKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQVSGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSA"
     gene            3335..>3919
                     /gene="rsmG"
                     /locus_tag="ACHKBL_004568"
     CDS             3335..>3919
                     /gene="rsmG"
                     /locus_tag="ACHKBL_004568"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HFO5821316.1"
                     /translation="MLNKLSLLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGR
                     FYALKGQMPEDEIALLPEEYQVESVVKLHVPALDG"
BASE COUNT          976 a          997 c         1016 g          930 t
ORIGIN      
        1 aagaaaatcc tatggaaaat tatctaatcg acaatctgga ccgtggcatc ctggaagcat
       61 taatgggcaa tgcgcgcacc gcttacgccg aactggcgaa acaatttggc gtcagtccgg
      121 ggacgattca cgttcgagta gagaaaatga agcaggcggg gatcattacc ggggcgcgta
      181 ttgatgtcag cccgaagcag ctcggttatg acgtaggctg ctttatcggc attatattaa
      241 agagcgccaa agactatcct tccgcgctgg cgaaactgga aagccttgat gaagtcactg
      301 aagcctatta caccaccggc cactacagca tctttataaa agtgatgtgc cgttcgatcg
      361 acgctctcca gcatgtactt atcaacaaga tccaaacaat tgatgaaatt cagtccaccg
      421 agacattgat cgtcctgcaa aacccgatca tgcgtaccat caagccctga tcggcttttt
      481 taatcccata cttttccaca ggtagatccc aacgcgttca cagcgtacaa tacgccactc
      541 ttaataaagg tggcggttta tggcagatat cactcttatc agcggcagca ccctcggcgg
      601 tgccgaatat gtagcagaac acctggctga aaagctggaa gaggcgggtt ttaccaccga
      661 aacgctgcac ggtccgctgt tggaagattt atctgcctct gggatctggc tggttatcag
      721 ctcaacccac ggtgccggag atattccgga caacctttct cctttctatg aagcattgca
      781 ggaacagaag cccgatcttt ctgcggtccg ctttggcgca atcggtattg gtagtcgtga
      841 atatgacacc ttttgtgggg ctatcgataa actcgaggca gaactcaaaa attccggtgc
      901 aaaacagaca ggcgaaacac tgaagatcaa cattcttgat cacgacattc cggaagatcc
      961 ggcagaagaa tggctgggat cgtggattaa tttactcaaa taagtataca gatcgtgcga
     1021 tctactgtgg ataactctgt caggaagctt ggatcaaccg gtagttatcc aaagaacaac
     1081 cgttgttcag tttttgagtt gtgtataacc cctcattctg atcccagctt atacggtcca
     1141 ggatcaccga tcattcacag ttaatgatcc tttccaggtt gttgatctta aaagccggat
     1201 ccttgttatc cacagggcag tgcgatccta ataagagatc acaatagaac agatctctaa
     1261 ataaatagat cttcttttta atacccagga tcccaggtct ttctcaagtc gacaaagttg
     1321 agtagaatcc acggcccggg cttcaatcca ttttcatacc gcgttatgcg aggcaatcac
     1381 catgttttat ccggatcctt ttgacgtcat catcattggc gggggtcatg caggcaccga
     1441 ggccgcgatg gccgcggcgc gtatgggtca acagactctg cttttgacac acaatatcga
     1501 cactctgggg cagatgagct gcaacccggc gatcggcggt attgggaagg gacatctggt
     1561 aaaagaagtg gatgcactcg gcggtctgat ggcgaaagcg atcgatcagg cgggtatcca
     1621 gtttaggata ctaaacgcaa gcaagggacc ggcggttcgc gctacccgag ctcaggcgga
     1681 tcgtgtgctc taccgtcagg cggtacgtac ggcgctggag aaccaaccga acctgatgat
     1741 cttccagcag gcggttgaag atcttattgt cgaaaacgat cgcgtggtcg gtgctgttac
     1801 ccaaatggga ctgaagttcc gtgccaaagc cgtcgtgctt accgttggga cgttcctcga
     1861 cggtaaaatt catatcggtc tggataacta cagcggtggt cgtgctggtg atccgccgtc
     1921 cattccgctc tctcgccgtt tgcgtgaact gccgctgcgc gttggtcgtc tgaaaaccgg
     1981 gacaccgccg cgtattgatg cgcgtactat cgacttcagc gtgctggcgc aacagcatgg
     2041 cgataaccca atgccggtat tctcgtttat gggtaatgcg tcccagcatc cacagcaggt
     2101 gccgtgttat atcacccata ccaacgagaa aacccatgat gtgatccgca gtaacctcga
     2161 tcgtagccca atgtatgcag gggtgatcga aggtgtcggc ccacgctact gcccgtcgat
     2221 cgaagacaaa gtcatgcgct tcgccgacag aaatcagcat cagatcttcc ttgaaccgga
     2281 agggctgacc tcaaacgaaa tttatccgaa cggtatctcc accagcctgc cgttcgatgt
     2341 gcagatgcaa atcgtccgct ccatgcaggg gatggaaaac gcgaagatcg tgcgtccggg
     2401 ttatgccatt gagtatgact tcttcgatcc acgcgacctg aaaccgacgc tggagagcaa
     2461 gtttatccag gggctgttct ttgctggtca gattaacggc actaccggtt acgaagaagc
     2521 cgctgcgcaa ggtttgctgg ccggtcttaa cgctgcccgt ctgtctgctg acaaagaagg
     2581 ttgggctccg gcgcgttctc aggcgtatct cggcgtactg gttgatgacc tgtgcacttt
     2641 aggaaccaaa gaaccgtatc gtatgtttac ctcgcgcgca gaatatcgtc tgatgctgcg
     2701 cgaagataat gcggatctgc gtttgactga aattggtcgt gaactgggcc tggtggatga
     2761 cgaacgttgg gcgcgcttta acgagaaact tgagaatatc gagcgtgagc gtcagcgtct
     2821 gaaatcgacc tgggtaaccc cgtcggcgga agctgcagcc gaagtgaatg ctcacctgac
     2881 tgcgccactt tcccgtgaag ccagtggtga agatctgctg cgtcgtccgg aaatgactta
     2941 tgaaaaatta accacgctga cgccgtttgc ccctgcgttg acagacgaac aggcggcgga
     3001 acaggttgag attcaggtta aatacgaagg ttatatcgcg cgccagcaag atgagatcga
     3061 aaagcagctg cgtaacgaga acaccctgct accagcgacg ctggattacc gccaggtatc
     3121 cggtctttct aacgaagtga tcgccaaact taacgatcac aaaccggcct ctatcggcca
     3181 ggcttcgcgt atttctggcg tcacgcctgc ggccatctcc attctgctgg tgtggctgaa
     3241 aaaacagggt atgctgcgtc gtagcgcata acgcattaaa aatgcctggt aagcacccgc
     3301 ttaccgggca acgcatcaag aacaggtaat caccgtgctc aacaaactct ccttactgct
     3361 gaaagacgca ggtatttcgc ttaccgatca ccagaaaaac cagcttattg cctatgtgaa
     3421 tatgctgcat aaatggaata aagcgtacaa cctgacttcg gtccgcgatc ctaatgagat
     3481 gctggtacgc catattctcg atagcattgt ggtggcaccg tatctgcaag gtgaacggtt
     3541 tatcgatgtc ggcaccggtc caggactgcc tggcattcca ctctctatcg tgcgtcctga
     3601 agcccatttc actctgttgg atagccttgg taaacgcgtg cgtttccttc gtcaggtgca
     3661 acatgagctt aaactggaga atattgagcc agtacagagc agggtagaag agtttccttc
     3721 agagccgcca tttgatggcg taattagccg cgcttttgcc tctctgaacg atatggtgag
     3781 ctggtgtcac catcttcctg gtgagcaagg ccgtttctac gcgctgaaag gccaaatgcc
     3841 ggaagatgaa atcgctttgt tgcccgaaga atatcaggtc gaatcagtgg ttaaacttca
     3901 cgttccggcc ctggatggc
//
LOCUS       DAZFLD010000296         3913 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.298, whole genome shotgun sequence.
ACCESSION   DAZFLD010000296 DAZFLD010000000
VERSION     DAZFLD010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3913)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3913
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.298"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            48..1706
                     /gene="dpiB"
                     /locus_tag="ACHKBL_004569"
     CDS             48..1706
                     /gene="dpiB"
                     /locus_tag="ACHKBL_004569"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase DpiB"
                     /protein_id="HFO5821317.1"
                     /translation="MLQLNENKQFAFFQRLAFPLRIFLLILVFSIFVIAALAQYFTAS
                     FEDYLTLHVRDMAMNQAKIIASNDSIISAVKTRDYKRLATIADKLQRDTDFDYVVIGD
                     RHSIRLYHPNPEKIGYPMQFTKPGALEKGESYFITGKGSIGMAMRAKTPIFDDDGKVI
                     GVVSIGYLVSKIDSWRAEFLLPMAGVFVVLLGILMLLSWFLAAHIRRQMMGMEPKQIA
                     RVVRQQEALFSSVYEGLIAVDPHGYITAINRNARKMLGLSSPGRQWLGKPIAEVVRPA
                     DFFTEQIDEKRQDVVANFNGLSVIANREAIHSGDDLLGAIISFRSKDEISTLNAQLTQ
                     IKQYVESLRTLRHEHLNWMSTLNGLLQMKEYDRVLAMVQGESQAQQQLIDSLREAFAD
                     RQVAGLLFGKVQRARELGLKMVIVPGSQLSQLPPGLDSTEFAAIVGNLLDNAFEASLR
                     SDEGNKIVELFLSDEGDDVVIEVADQGCGVPESLRDKIFEQGVSTRADEPGEHGIGLY
                     LIASYVTRCGGVITLEDNDPCGTLFSIYIPKVKPNDSSINPIDR"
     gene            1675..2355
                     /gene="dpiA"
                     /locus_tag="ACHKBL_004570"
                     /gene_synonym="citB"
     CDS             1675..2355
                     /gene="dpiA"
                     /locus_tag="ACHKBL_004570"
                     /gene_synonym="citB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component response regulator DpiA"
                     /protein_id="HFO5821318.1"
                     /translation="MTAPLTLLIVEDETPLAEMHAEYIRHIPGFSQILLAGNLAQARM
                     MIERFKPGLILLDNYLPDGRGINLLHELVQAHYPGDVVFTTAASDMETVSEAVRCGVF
                     DYLIKPIAYERLGQTLTRFRQRKHMLESIDSASQKQIDEMFNAYARGEPKDELPTGID
                     PLTLNAVRKLFKEPGVQHTAETVAQALTISRTTARRYLEYCASRHLIIAEIVHGKVGR
                     PQRIYHSG"
     gene            complement(2396..3781)
                     /gene="dcuC"
                     /locus_tag="ACHKBL_004571"
     CDS             complement(2396..3781)
                     /gene="dcuC"
                     /locus_tag="ACHKBL_004571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HFO5821319.1"
                     /translation="MLTFIELLIGVVVIVGVARYIIKGYSATGVLFVGGLLLLIISAI
                     MGHKVLPSSQASTGYSATDIVEYVKILLMSRGGDLGMMIMMLCGFAAYMTHIGANDMV
                     VKLASKPLQYINSPYLLMIAAYFVACLMSLAVSSATGLGVLLMATLFPVMVNVGISRG
                     AAAAICASPAAIILAPTSGDVVLAAQASEMSLIDFAFKTTLPISIAAIIGMAIAHFFW
                     QRYLDKKEHISHEMLDVSEITTTAPAFYAILPFTPIIGVLIFDGKWGPQLHIITILVI
                     CMLIASILEFIRSFNTQKVFSGLEVAYRGMADAFANVVMLLVAAGVFAQGLSTIGFIQ
                     SLISIATSFGSASIILMLVLVILTMLAAVTTGSGNAPFYAFVEMIPKLAHSSGINPAY
                     LTIPMLQASNLGRTLSPVSGVVVAVAGMAKISPFEVVKRTSVPVLVGLVIVIVATELM
                     VPGTAAAVTGK"
BASE COUNT         1062 a          935 c         1015 g          901 t
ORIGIN      
        1 ttaattacat tcgtcaaaga ggctcgggac aacccgcaag gaaaacaatg ttgcagctta
       61 acgagaataa acagtttgca tttttccaaa gactggcatt tccgctgcgt atctttttgc
      121 tgattctggt gttctcaata tttgtcattg cagccctggc gcaatatttt acggccagtt
      181 ttgaggacta tttaacgctt catgtgcgcg atatggcaat gaatcaggca aaaattattg
      241 cttctaatga cagtatcatc agcgcagtga aaacgcgtga ctacaagagg ctggcgacca
      301 tcgctgataa attacaaaga gatactgatt tcgattatgt ggtgattggc gatcggcact
      361 cgattcgtct ttaccatcct aatccggaga aaattggtta tcctatgcag ttcaccaaac
      421 cgggcgcgct ggagaaaggg gagagctact tcatcaccgg gaaagggtca attggcatgg
      481 cgatgcgtgc caaaacgcca atctttgatg acgatggaaa agtcatcggc gtggtgtcga
      541 ttggctacct ggtgagtaaa atcgatagct ggcgggctga gtttttatta ccgatggctg
      601 gcgtgtttgt cgtgctgtta gggattctga tgttgctgtc gtggttcctg gccgcgcata
      661 tccgtcggca gatgatgggc atggagccaa agcaaatcgc acgcgtggtc cgtcagcaag
      721 aggcgctgtt tagttcggtt tatgaagggc tcattgcggt ggatccgcac ggttacatta
      781 ccgccatcaa tcgtaacgca agaaagatgc tggggctgag ctcccccgga cggcaatggt
      841 tgggtaaacc cattgctgaa gtggtcaggc ccgccgattt ctttaccgaa cagattgatg
      901 aaaaacgtca ggatgtggtg gcgaacttta acggtctgag cgttattgcc aaccgggaag
      961 ctattcattc tggtgatgat ttgctggggg ccattatcag ctttcgtagt aaagacgaaa
     1021 tatccaccct caatgcacaa ctgacgcaaa ttaaacaata cgtcgagagc ctgcgcacat
     1081 tgcgacacga gcatctcaat tggatgtcga cgctcaatgg tctgttgcag atgaaagagt
     1141 atgatcgcgt gttggcgatg gtgcaggggg agtctcaggc ccagcaacag cttattgata
     1201 gcctgcgcga ggcgtttgcc gatcgccagg tggcggggct gctttttggt aaagtgcagc
     1261 gcgcccgcga actggggcta aaaatggtca tcgtccccgg aagtcagctt tcgcaactgc
     1321 cgccaggact ggacagcacc gagtttgcag ccattgttgg caatttactt gataacgcct
     1381 tcgaagccag cctgcgtagc gatgaaggaa acaagatcgt tgaattattc ctcagcgatg
     1441 aaggcgatga tgtggtgatt gaagtcgccg atcagggctg cggcgttcca gagtctctac
     1501 gagacaaaat atttgagcag ggtgtcagta cgcgtgctga cgagcccggc gaacatggca
     1561 ttgggttgta cttgattgcc agctacgtaa cgcgctgcgg tggtgttatc actctcgaag
     1621 ataatgatcc ctgcggtacc ttattttcaa tctatattcc gaaagtgaaa cctaatgaca
     1681 gctccattaa ccctattgat cgttgaggac gaaacgccgc tggcagagat gcatgcggaa
     1741 tatattcgtc acattcccgg attcagtcag atattactgg cgggaaacct ggcgcaggcc
     1801 cgaatgatga tcgagcgttt taagccgggg ctaatcttgc tcgataacta tcttcctgac
     1861 ggtagaggga ttaatttact gcatgaactg gtgcaggcgc attatcccgg cgacgtggtg
     1921 tttaccactg cagccagtga tatggaaacg gtgtctgaag ccgtacgttg tggtgtattt
     1981 gattatctca ttaagccaat tgcctatgaa cggctggggc aaacgctaac ccgtttccgc
     2041 cagcgtaaac atatgctgga aagtattgat agcgccagcc agaagcaaat cgatgagatg
     2101 tttaatgcct atgctcgcgg tgaacccaaa gacgaactgc cgaccggcat tgaccccttg
     2161 acgctaaacg cggtgcgaaa actgtttaaa gagcctggtg tgcaacatac ggcagaaacg
     2221 gtggcgcagg cactgactat cagccgcacc actgccagac gttatcttga atattgcgcc
     2281 agccgccatc tgattattgc tgaaattgtt cacggcaaag ttggcagacc acaacgcata
     2341 taccacagtg ggtgacatga taaagccgga ggaaacttgc ctccggcatg actatttact
     2401 tgcctgtgac cgcggctgcc gttcctggca ccatcagctc tgtagcaacg ataacaatca
     2461 ccagcccaac aagcaccggc accgaggtgc gttttacgac ttcaaatggt gagattttcg
     2521 ccatcccggc aaccgcaacg actacgccag aaacgggtga cagggtacgg ccgaggtttg
     2581 acgcctgcag cattgggata gtcaaatacg ccgggttaat gccggaagag tgcgccagtt
     2641 tcgggatcat ctcaacaaac gcataaaacg gcgcattgcc tgaaccggtc gtgactgccg
     2701 ccagcattgt caggatcacc aataccagca tcaggatgat gctcgccgaa ccaaacgagg
     2761 tagcgataga aatcagactt tgaataaagc cgatggtgct aagcccctga gcgaataccc
     2821 cagcggcaac cagcagcatc accacgtttg caaatgcatc tgccatcccg cgataagcca
     2881 cttccagacc agagaaaact ttctgggtat taaagctgcg gatgaactcc agaatggagg
     2941 caatcagcat acaaatcacc agaatagtga tgatgtgtaa ttgcggaccc catttgccgt
     3001 caaaaatcag cactccgatg atcggcgtga acggcaaaat ggcataaaac gcaggggcag
     3061 tggtggtgat ttcactgaca tctaacattt catgagagat gtgttctttt ttatccagat
     3121 aacgttgcca gaagaagtgg gcgattgcca tgccgataat tgcagcaatt gagataggca
     3181 gcgttgtttt gaaggcgaag tcaatcagcg acatttcgga agcctgcgcc gccagcacca
     3241 catcccctga agtcggtgcg agaataatcg ccgccgggga ggcacaaata gcagcagctg
     3301 caccacggct gataccaacg tttaccatca ccggaaacag ggttgccatc agcaaaacac
     3361 ccagaccggt cgcggaagag acggctagag acatcagaca ggcgacaaaa taggcggcaa
     3421 tcatcagcag gtaaggggag ttaatatact gcaatggttt tgacgccaac ttgaccacca
     3481 tatcattcgc gccgatatgg gtcatgtaag cggcaaagcc acacagcatc ataatcatca
     3541 tgccgaggtc gccgccgcgg ctcattagca atattttaac gtattcaacg atatccgtgg
     3601 cgctgtagcc tgttgaagcc tggctggacg gtaacacttt gtgccccata atggcactga
     3661 taatcagcaa taacaggcca ccgacaaata acacgccagt ggcggaatac cctttaatga
     3721 tgtagcgagc tacacccaca ataaccacaa ccccaataag gagttcaatg aatgtcagca
     3781 taatttttcc tgtctccagg ccccaaagta aataataaaa aattcctaaa gcttaaggaa
     3841 aaaatatgcc caataaattg gcgatgaatg ctgattaaaa tcaagaaaaa ctgccattaa
     3901 gacattgaag ttg
//
LOCUS       DAZFLD010000297         3909 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.299, whole genome shotgun sequence.
ACCESSION   DAZFLD010000297 DAZFLD010000000
VERSION     DAZFLD010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3909)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3909)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3909
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.299"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(196..1188)
                     /locus_tag="ACHKBL_004572"
     CDS             complement(196..1188)
                     /locus_tag="ACHKBL_004572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000023738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO5821320.1"
                     /translation="MSIKQLKDGRYKVDVRPQGTEGKRIRKIFTLKSKAQEFERYVLQ
                     NFHDKPWQAKPADQRRLSELIEAWWLLDGRNQDYGDTYRVRLEKINREMGDPRASQMT
                     RKFMLGYRSDKLQAGLMPSSINRDLCALSAMFSSLIEGEVFHNENPVRGIRKLKVRNT
                     EMAFLSDEEIERLLERLDGDARRVAILCLSTGARWGEAAGLRGEHIVGNRVMFFNTKN
                     GKSRAVPVSDAVLALIKTRRTGLLYQADYLRFREILQEVKPDLPKGQATHVMRHTFAT
                     HFMMNGGNIVTLQRILGHATIQQTMTYAHFSPDFLQDAISFNPLAESVHKLSID"
     gene            complement(1258..1599)
                     /locus_tag="ACHKBL_004573"
     CDS             complement(1258..1599)
                     /locus_tag="ACHKBL_004573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO5821321.1"
                     /translation="MVILSIDYAEKLRQIRKAEGLTQKQFAEITGLSLATVRNYESGQ
                     KSARAKIVESVLQADRFEKYMFWLLKDKTLPAAGQIAPALSLDGSSQSEGDQASIDVT
                     QKSSRSGRNAG"
     gene            1703..2227
                     /locus_tag="ACHKBL_004574"
     CDS             1703..2227
                     /locus_tag="ACHKBL_004574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cox family DNA-binding protein"
                     /protein_id="HFO5821322.1"
                     /translation="MRDFIADKELSDTDHTTGAAYINADTSQQEATDNNDEQKPQRTY
                     KKGATLRLDGPIAGLCSLEKGAAYIGLTKTALRAALQRNQMPGHKTRSNPEDENSDGV
                     WWFNVREWDKLADELPECEPPEWHNWKSYWTYDRQKRKFTPADKEDCQTVNGKRVYKG
                     RKSKLEQSQDVNER"
     gene            2234..2629
                     /locus_tag="ACHKBL_004575"
     CDS             2234..2629
                     /locus_tag="ACHKBL_004575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5821323.1"
                     /translation="MSGKNKSEKNATVLKQLSLLKEEHKDATILMHLEGSPKITITTT
                     YSELHKELEGLINFCSKLKANENIDDLILKPEAEFGHFCESRENGCIGIMLYATRGIM
                     INLPVEQAVDIFGEMALNKTTIYKSKTTE"
     gene            2650..3168
                     /locus_tag="ACHKBL_004576"
     CDS             2650..3168
                     /locus_tag="ACHKBL_004576"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5821324.1"
                     /translation="MTITPEEEGMHKKYSEHGSKAGEVIAIDEDCLITGDPWLATERD
                     LEGKTFFVRAIKHKDRVVGGIIYSTSTIIPDNYLDISFRPVSIQGCEAYLNYVAELKE
                     INLDYMWPIVQREIMRRKSEGFNVKIEFLSTLMGFLQKPLILFPSLDRNLPVKEGVEK
                     YIVEQVRDLVCL"
     gene            complement(3146..3469)
                     /locus_tag="ACHKBL_004577"
     CDS             complement(3146..3469)
                     /locus_tag="ACHKBL_004577"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5821325.1"
                     /translation="MIDKSVKDAITEYLVSIYTTNLAVAPVDKLTDPRDSVGVVSDLL
                     ISLLISELPDDKRDALIKTASEILRQIEGQQAFAPYAVNIRTRFHSINHLACFIRASK
                     DKPDL"
     gene            3578..>3909
                     /locus_tag="ACHKBL_004578"
     CDS             3578..>3909
                     /locus_tag="ACHKBL_004578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000194243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4761 family protein"
                     /protein_id="HFO5821326.1"
                     /translation="MTQRRSFRTGTERHAARFSTSASRSNSYYSLQETHATPDGHLVK
                     QTGKHTWLINDAGIVIHRGARNPITGNTCYSLTRGDEQFGQDFTLHEALRTADRLISG
                     RSFQHPEHR"
BASE COUNT         1183 a          924 c          879 g          923 t
ORIGIN      
        1 tgaaaatcaa agagataggt agattttcac tcgaaagtag tattacgtgt aagtgaggag
       61 taattattgt tcggtatcaa agggagacaa aaaaagcgcc cgcaggaggt ttaacgctac
      121 ttactcccct tgatgatttg cttatttttc aatgagtaaa tcaaaacaag gggagcaaat
      181 aagcacaagt tacaactaat cgatggacag tttatggaca ctttcagcca gcggattaaa
      241 gctgattgca tcctgtaaaa agtccggcga aaaatgcgca tacgtcatcg tttgttgaat
      301 cgtggcatgg cccaggattc gttgtagcgt aacgatattt ccgccattca tcatgaaatg
      361 tgtagcgaat gtatgacgca tgacatgggt ggcctgccct ttcggtaagt ccggcttcac
      421 ctcctgaagg atctcccgaa atctgaggta atcagcctgg tacaacaatc cggttctgcg
      481 ggttttaatt aacgcaagta ccgcatcaga aacgggaacg gcgcgagatt ttccgttctt
      541 ggtgttaaag aacatcacgc ggttacctac aatatgttcc ccacgtaaac cagctgcttc
      601 accccacctt gcccccgtgg aaagacacag aatggcaaca cgccgcgcat ctccatcgag
      661 tcgttcaagc agtcgttcaa tctcctcatc tgaaagaaaa gccatctctg tgttcctgac
      721 tttcagctta cgtattcccc gaaccgggtt ttcgttatgg aacacctcac cctcaatcag
      781 cgagctgaac attgcagaca aggcacacaa atcgcggtta atgctggaag gcattaaccc
      841 ggcctgtaat ttgtcggacc ggtacccgag cataaatttt cgcgtcatct gactggcacg
      901 aggatcaccc atctcacggt ttatcttttc cagtctgacc ctgtaggtat ccccgtaatc
      961 ttggttacga ccatcaagca accaccaggc ctctatcagt tcggataacc gacgctgatc
     1021 tgctggctta gcctgccatg gcttatcatg aaaattctgc aaaacatacc tttcaaactc
     1081 ctgcgccttt gacttcaggg taaaaatctt acgaatccgc tttccttccg tcccttgtgg
     1141 ccttacatca accttataac gtccgtcttt gagttgctta atcgacatga ttagcccctc
     1201 caacggataa attaaccaaa cactccctgc gggatgaaac ccgctcgatg tgtaacgtca
     1261 gccagcattg cggcctgatc gggatgattt ttgggtaacg tcgattgaag cctgatcgcc
     1321 ctccgactga gaagatccat caagagagag agccggggca atttgcccgg cggcaggtag
     1381 cgtcttatcc tttaacagcc aaaacatata cttttcaaat cgatctgcct gaagaaccga
     1441 ctccacgatt ttagctctgg cgctcttttg ccctgattca taattcctta cggtggccag
     1501 agataaccct gtaatttcag caaattgttt ttgcgttaag ccctccgcct tacgtatctg
     1561 gcgtaatttt tccgcgtagt ctattgacaa gataaccatt tgagtattaa cctctcgaaa
     1621 aagataacct ttcgattatc tttacaaacc aaaacaaacc ttcataaggt gcaataggcg
     1681 cacctgtgcg agaggataac aaatgcggga cttcattgcc gataaagagt tatctgacac
     1741 cgatcacaca actggagcgg cgtacatcaa cgccgatacc agtcaacaag aagcaacaga
     1801 taacaacgac gaacaaaagc cacagcgcac atataaaaaa ggggcaaccc tgcggctgga
     1861 tggcccaata gccgggcttt gctcacttga aaaaggggcc gcctacatcg gattaacaaa
     1921 aacagcatta cgcgcagctc tccagcgtaa ccagatgcca gggcataaaa cccgctcaaa
     1981 ccctgaagat gagaattcgg atggtgtttg gtggtttaac gtcagagagt gggacaagct
     2041 ggccgacgaa ctcccggaat gcgagccacc agaatggcac aactggaaaa gctactggac
     2101 atacgaccgc cagaaaagaa agtttacccc ggctgataag gaagattgcc agaccgtcaa
     2161 tggaaaacgg gtttacaaag gcagaaagtc aaagctggaa cagagccagg atgtaaacga
     2221 acggtgatga acgatgagcg gcaaaaataa atcagaaaaa aacgcaaccg tccttaagca
     2281 gttaagcctc ctgaaagagg aacacaagga cgccacgata ctgatgcatc tggaaggctc
     2341 accgaaaata acaatcacaa caacctatag cgagttacac aaagagcttg aaggattaat
     2401 taacttttgt tcaaagctaa aagcgaatga aaacattgat gacctgatac ttaaaccaga
     2461 agcagagttc ggacactttt gcgaaagtag agaaaatgga tgcatcggaa taatgctata
     2521 cgcaacccgt ggaataatga ttaatctgcc cgttgagcag gctgtggata tattcggaga
     2581 aatggcactt aataaaacca ctatttacaa aagcaaaacg acggaataac ccggcacgaa
     2641 ggaggatata tgacgattac accagaagaa gaaggtatgc ataagaaata cagcgaacac
     2701 gggagtaagg ctggcgaagt aatcgccatt gatgaggatt gcctgataac aggagacccc
     2761 tggctggcaa cagaaaggga tttagaaggc aaaacatttt ttgtcagagc aataaagcac
     2821 aaagataggg ttgtgggtgg aattatttat tcaaccagta cgattattcc tgataattat
     2881 ctggatattt cttttagacc tgtaagcatt caggggtgcg aggcatacct taattatgtt
     2941 gcggagctaa aggaaattaa cctggactac atgtggccaa tcgtacaacg cgaaataatg
     3001 cgacgaaaat cagaaggctt caatgtgaaa attgagtttt tatcgacgtt aatgggattt
     3061 ttacaaaaac cattgattct tttcccctcc cttgaccgga acctgccagt caaagaaggg
     3121 gtggaaaaat atattgttga acaagtcaga gatctggttt gtctttagag gcgcgaataa
     3181 agcacgcaag atgattaata ctatgaaagc gtgtccttat attcacggca tagggagcaa
     3241 aggcttgttg cccctctatc tgacgcaaaa tttctgacgc agtttttatc aatgcatccc
     3301 ttttatcatc gggaagttcg ctaatgagga gcgaaataag cagatcggac accactccca
     3361 ctgagtctct tgggtcggtc aacttatcaa caggagccac agcaagatta gttgtgtaaa
     3421 tgctaaccag gtattcagtg atagcgtcct taacgctttt atcaatcatg ttagttacct
     3481 tttttctggt tgtgtggaaa caccagaata ccacgcgccg ggcgtggtta aacatcccgg
     3541 cactgatttt catttaactt aaacggagtt ttccgatatg acacagagac gcagcttccg
     3601 cactggcaca gaacgccacg cagcccgttt ctcaaccagc gcatcacgca gcaactcgta
     3661 ctacagcctg caggaaacgc acgccacacc tgacgggcat ctggttaaac aaaccggtaa
     3721 acacacctgg ctgattaatg atgccggaat tgtcatacac agaggcgcac gcaacccgat
     3781 taccggaaat acctgttact ccctgactcg cggagatgag cagttcgggc aggatttcac
     3841 cctgcacgaa gcattaagaa cggcagaccg gttaatcagc ggtcgcagtt ttcagcaccc
     3901 ggaacatcg
//
LOCUS       DAZFLD010000298         3869 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.300, whole genome shotgun sequence.
ACCESSION   DAZFLD010000298 DAZFLD010000000
VERSION     DAZFLD010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3869)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3869)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3869
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.300"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            2..1510
                     /gene="mltF"
                     /locus_tag="ACHKBL_004579"
     CDS             2..1510
                     /gene="mltF"
                     /locus_tag="ACHKBL_004579"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HFO5821327.1"
                     /translation="MLAVALWPSIPWFGKADNRIAAIQARGELRVSTIHTPLTYNEIN
                     GKPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNADLLAAGLVYNSERVKN
                     YQPGPTYYSVSQQLVYKVGQYRPRTLGNLTAEQLTVAPGHVVVNDLQTLKETKFPELS
                     WKVDDKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELAVALDITDEQPVTWFSP
                     LDGDNTLSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVDTRTFLRAVDAVLPQLK
                     PLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLTKNTAQSLGITDRTDAE
                     QSISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHMLDARALTAKTKGNPDSW
                     ADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQISLVGYLQEKEKQATEAA
                     MQLAQDYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLFSRKGSEEKQN"
     gene            complement(1507..2010)
                     /gene="tadA"
                     /locus_tag="ACHKBL_004580"
     CDS             complement(1507..2010)
                     /gene="tadA"
                     /locus_tag="ACHKBL_004580"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HFO5821328.1"
                     /translation="MSEVEFSHEYWMRHALTLAKRAWDEREVPVGAVLVHNNRVIGEG
                     WNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCAGAMIHSRIGRV
                     VFGARDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFFRMRRQEIKAQK
                     KAQSSTD"
     gene            complement(2068..2703)
                     /gene="yfhb"
                     /locus_tag="ACHKBL_004581"
     CDS             complement(2068..2703)
                     /gene="yfhb"
                     /locus_tag="ACHKBL_004581"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311453.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase C"
                     /protein_id="HFO5821329.1"
                     /translation="MATHERRVVFFDLDGTLHQQDMFGSFLRYLLRRQPLNALLVLPL
                     LPIIAIALLIKGRAARWPMSLLLWGCTFGHNEARLQALQADFVRWFRDNVTAFPLVQE
                     RLTTYLLSSDADIWLITGSPQPLVEAVYFDTPWLPRVNLIASQIQRGYGGWVLTMRCL
                     GHEKVAQLERKIGTPLRLYSGYSDSNQDNPLLYFCQHRWRVTPRGELQQLE"
     gene            2912..3760
                     /locus_tag="ACHKBL_004582"
     CDS             2912..3760
                     /locus_tag="ACHKBL_004582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFO5821330.1"
                     /translation="MNGLLRIRQRYQGLAQSDKKLADYLLLQPDTARHLSSQQLANEA
                     GVSQSSVVKFAQKLGYKGFPALKLALSEALASQPESPSVPIHNQIRGDDPLRLVGEKL
                     IKENTAAMYATLNVNSEEKLHECVAMLRSARRIILTGIGASGLVAQNFAWKLMKIGFN
                     AAAVRDMHALLATVQASSPDDLLLAISYTGVRRELNLAADEMLRVGGKVLAITGFTPN
                     ALQQRASHCLYTIAEEQATNSASISACHAQGMLTDLLFIALIQQDLELAPERIRHSEA
                     LVKKLV"
BASE COUNT          997 a         1009 c          964 g          899 t
ORIGIN      
        1 gctgctcgcg gtcgctctct ggccatccat tccctggttt ggtaaagccg acaaccgtat
       61 cgccgccatt caagcgcggg gagagttgcg tgtgagcacc attcataccc ccctgactta
      121 taacgaaatc aacgggaaac cttttggcct ggattacgaa ctggcgaaac agtttgccga
      181 ttacctcggc gtaaaactga aagtgaccgt gcggcagaat atcagccagc tgtttgacga
      241 cctcgataat ggtaacgccg acctgctggc ggcaggcctg gtctataaca gtgagcgtgt
      301 aaaaaattat cagcctggcc ctacctatta ttccgtgtca caacaactgg tttataaagt
      361 gggtcagtat cgcccacgta cactgggcaa cctgacggcg gagcaactca ccgttgcacc
      421 gggtcatgtg gtggttaacg atctccagac cctgaaagaa acaaaattcc cggaattaag
      481 ctggaaggta gacgacaaaa aaggctctgc ggaattaatg gaagatgtca tcgaaggaaa
      541 actcgattac accattgctg attctgtcgc catcagcctg tttcagcgcg ttcacccgga
      601 actcgccgta gcgctcgata tcaccgatga acaaccggtg acctggttta gcccgttaga
      661 tggcgataat accctttccg ccgctctgct cgatttcttc aatgagatga atgaagacgg
      721 tacgctggca cgcattgaag agaaatacct ggggcatggc gatgattttg attacgtcga
      781 tacgcgcaca tttttacgcg ccgtcgatgc ggtactgccg cagttaaagc ccctgtttga
      841 gaaatacgcc gaagaaattg actggcgttt gctggccgct attgcttatc aagaatcgca
      901 ctgggatgct caggccactt caccgacggg tgtgcgcggc atgatgatgt taaccaaaaa
      961 caccgcgcaa agcctcggca ttacggatcg taccgatgcc gaacagagca tcagcggcgg
     1021 cgtgcgttat ttgcaggata tgatgagtaa agtgccggaa agtgtgccgg agaacgaacg
     1081 gatctggttt gcccttgccg cgtacaatat gggctatgcg catatgctgg atgcccgcgc
     1141 cctgacggca aaaaccaaag gtaatcctga cagttgggct gacgtaaaac agcgtctgcc
     1201 tttacttagc cagaaaccct attacagcaa gctgacttac ggctacgctc gtgggcatga
     1261 agcctacgct tatgtcgaaa atattcgtaa gtatcagatt agcctggtgg gttatctgca
     1321 ggagaaagag aagcaggcta cagaagcggc gatgcaactg gcgcaggatt atccggcggt
     1381 atcgcctacg gagctgggca aagagaaatt tccttttctc tcgtttcttt cccagtcgtc
     1441 atcaaactat ttgacccact ctccctctct gctgttttcc agaaaaggga gtgaagagaa
     1501 acaaaattaa tctgtcgagg attgcgcttt tttctgcgct ttaatttcct ggcggcgcat
     1561 gcgaaagaag tcactgagca acgccgcgca ctcatccgcc agtattcctt cggtaatttc
     1621 cacccggtga ttcatacccg gatgatgcag cacatccatt aaagatcccg cagcgccagt
     1681 tttcgcgtca cgcgcaccaa agaccacgcg accaatgcga ctgtggatca ttgctccggc
     1741 acacattaca cagggttcaa gcgtgacata cagcgtggcg tcgatcagtc gataattttg
     1801 catcaccaga ccgccctgcc gcagggccat gatttctgca tgtgcggtgg gatcatgtcg
     1861 accaatcgga cggttccagc cttcgccaat aacacgattg ttatgcacta ataccgcgcc
     1921 gaccggcact tcccgctcat cccaggcacg ctttgccagc gtcagcgcgt gacgcatcca
     1981 gtattcgtgg ctaaattcga cttcagacaa aaagaaaact ccggttataa aagcgcggcg
     2041 cattatacac ggacgctatg ctttactcta ttccagttgc tggagttcac cgcgcggggt
     2101 tactcgccaa cgatgctgac agaaataaag cagcggattg tcctgattac tgtcgctata
     2161 gccactgtac agccgcagcg gcgtgccgat tttgcgctcc agttgtgcga ccttttcatg
     2221 tcccagacaa cgcatcgtca atacccaacc accatagcca cgctgaattt ggctggcgat
     2281 aagattaacc cgcggcagcc agggcgtatc gaaataaacc gcttcaacca gcggctgcgg
     2341 agagccggta atcaaccaga tatcagcatc ggaacttaac aggtaggtgg ttaatcgctc
     2401 ctgaaccagc ggaaaggcgg taacattgtc acgaaaccag cgtacgaaat cggcctgcaa
     2461 cgcctgcaaa cgcgcttcat tgtgaccaaa agtgcatccc cacagaagca gactcatcgg
     2521 ccagcgtgcc gcacgacctt ttatcaataa cgcaatggct atgatcggca acaacggcag
     2581 gacaagtaac gcattcagcg gttggcgacg cagtaaataa cgcagaaaac tgccgaacat
     2641 atcctgctga tgcaatgttc catctaagtc aaaaaacacc acacgacgct cgtgagttgc
     2701 caaaccatac tcctctggac tgaaacatcc tgattaattt tcgctactac atagcctaac
     2761 agatagatca tcacttttcc ggcaacaatc gaccgcatca tggctaactg gaatttttaa
     2821 ttcatggcaa ttagcggcaa tggaatataa aattcactcg cgtgtgtctc atattgcctg
     2881 ttgtcgccac tatttcatcg cgaggaaaga tatgaacggc ttacttcgta tccgtcagcg
     2941 ttaccagggg cttgcccaaa gtgataaaaa actggcggat tatctgctgc tacaacctga
     3001 tacggcgcgc catttaagct ctcagcaact ggccaacgaa gccggagtca gtcagtccag
     3061 cgtcgtgaag ttcgcgcaaa aactcggcta taaaggtttt ccggcactta agttggcgtt
     3121 gagtgaagcg ctggcaagcc agccggaatc tccctccgtg cccattcata accaaatccg
     3181 cggtgatgat ccgttacggc tggtcggcga aaaactgatt aaagaaaata ctgccgctat
     3241 gtacgcaacg ttaaacgtta atagtgaaga gaaactgcat gaatgcgtgg caatgttgcg
     3301 ctctgcgcgg cggataattc tgaccggtat tggcgcttcg ggtctggtgg cgcaaaactt
     3361 tgcctggaag ctgatgaaga ttggcttcaa tgctgccgca gtgcgcgata tgcatgcgct
     3421 actcgctaca gtacaggcgt cgtcccctga cgatctgtta ttagccattt cctacaccgg
     3481 tgtacgacgc gagttaaacc tggcggcaga tgagatgctg cgagtgggcg gaaaagtgct
     3541 ggcgattacc ggctttactc ccaatgccct gcaacagcgt gcttctcatt gcctgtatac
     3601 cattgccgaa gaacaagcga caaacagtgc ttcaatctct gcttgtcacg cgcagggaat
     3661 gttaacggat ttgctgttca ttgcgctgat tcagcaggat ctggaactgg ctccagaacg
     3721 tattcgtcac agtgaagcgc tggtgaaaaa actggtctga gtaaagaatg cgcgtataat
     3781 gcccgcccgg tttgtgttgt tttgagagtt tccttatggc gctgttaatc actaaaaaat
     3841 gcatcaattg tgatatgtgt gaaatcata
//
LOCUS       DAZFLD010000299         3858 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.301, whole genome shotgun sequence.
ACCESSION   DAZFLD010000299 DAZFLD010000000
VERSION     DAZFLD010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3858
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.301"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..867
                     /locus_tag="ACHKBL_004583"
     CDS             <1..867
                     /locus_tag="ACHKBL_004583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO5821331.1"
                     /translation="LCGSLRAAARKMNISQPALSRTIKELENVLGCNLLIRTRDGIVL
                     TEAGKIFSVHSRMILEEIETAALEVAKVNIDNTGKVAFGISSLFGVTILNNVLDNFRM
                     KYPKKRVMIREGQLSSLIPFLREGVLDFAMGTLTKDMPVGDFIVVELFNAPFTIVARK
                     HHPLRNCTSIDELATARWVFPETQMGYYHYISRLIPFGHPDEPYAPIFTDSVVCIMNL
                     VLYDDYITVLSRARLQEARFKEILCSIPVKYQLPVSQYGLIYPRKRLMTAPAQIMIEY
                     FQQEIQRYDWNG"
     gene            1432..2265
                     /gene="iolR"
                     /locus_tag="ACHKBL_004584"
     CDS             1432..2265
                     /gene="iolR"
                     /locus_tag="ACHKBL_004584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="myo-inositol utilization transcriptional
                     regulator IolR"
                     /protein_id="HFO5821332.1"
                     /translation="MSKHQTQLSLLQDDIRSRYDSLSKRLKQVAQYILDNSNSVAFDT
                     VASIAQHAEVPPSTLIRFANAFGFSGFNEMKQVFRQHLMEETVSYTERARLFRQKNIN
                     DGEPTPEKPGEILKLFSMVNAQVLQQLPMQIKNEQLDAAVSLLSKADNIYVIGLRRSF
                     SVACYLTYALRHLERRAFLIDGIGGMFSEQLSMISSNDVVIAISYSPYAQEVVELVEL
                     GAKRDAHHIVITDSQVSPLAAFSEVCFVIREAQVDGFRSQVASMCLAQTLAVSLALAA
                     E"
     gene            complement(2318..3751)
                     /gene="iolT1"
                     /locus_tag="ACHKBL_004585"
     CDS             complement(2318..3751)
                     /gene="iolT1"
                     /locus_tag="ACHKBL_004585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000495231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="myo-inositol import MFS transporter IolT1"
                     /protein_id="HFO5821333.1"
                     /translation="MPISVNRYNTGYILRICSIAALGGILFGYDTAVISGAISSLTSY
                     FHLSPAETGWAVSSVVVGCVVGSFSSGYLSKRFGRKKSLMISALLFTISAVGTSLSYT
                     FLHFIIYRIVGGLAVGLAATVSPMYMSEVSPKDMRGRALSMQQFAIVFGQILIFYVNY
                     KIASIATNTWLIEIGWRYMFAAGIAPCILFCILVFFIPESPRWMMMAGREEETLKILT
                     KISNEEHARHLLTDIKTSLQNDRLTAHQKLNYHDSNVRFILFLGCMIAMLQQVTGVNV
                     MMYYAPIVLRDVTGSAQEALFQTIWIGVIQLVGSIIGAMIMDKMGRLSLMRKGTIGSI
                     IGLLLTSWALYSQTTGYYALFGMLFFMIFYALSWGVGAWVLISEIFPNRMRSQGMSIS
                     VGFMWIANFMVSQFFPIINENPWLLAHFHGAFPMWIFAACCVFSYFFICRYLPETKGV
                     SLEKMEDIVLTKRRNKPQPIQAERSSD"
BASE COUNT         1142 a          793 c          862 g         1061 t
ORIGIN      
        1 ctgtgcggaa gtttaagagc tgctgcccgt aaaatgaata tatctcagcc agcattaagc
       61 agaacgataa aagaactgga gaatgtatta ggatgtaacc ttctgattcg aacaagggat
      121 ggtatcgttt taacagaagc tggaaagata ttctcggtcc attcacgtat gattcttgaa
      181 gagattgaaa ctgcagctct tgaagttgcg aaagtaaata tagataacac aggaaaagta
      241 gcttttggaa tttcttcgct gtttggcgta acaatactga ataatgtact tgataacttc
      301 cggatgaaat atccgaaaaa gcgtgtgatg ataagagagg gacagttgtc ctcccttatc
      361 cctttcctga gagaaggtgt tctggatttt gcaatgggta cattaacaaa agacatgcca
      421 gttggagatt ttattgtggt tgaattgttt aacgcaccgt tcactatagt ggcaagaaaa
      481 caccatcctt taaggaactg tacatcaatc gatgaacttg caactgcaag atgggtattc
      541 ccggaaactc aaatgggata ctatcattac atatcccgtc ttattccctt tggacatccg
      601 gatgaaccat atgctccaat atttaccgat tcggtagttt gtattatgaa tcttgttctc
      661 tatgatgact atataacggt tctctccaga gcaaggttac aggaagcaag attcaaagaa
      721 atattgtgtt caattcctgt taagtatcaa ctacccgtta gccagtacgg gcttatttat
      781 ccacgaaaaa ggctaatgac ggcaccagca cagataatga ttgaatattt tcaacaagag
      841 atacaacgat atgactggaa tggatgagca atacccggaa tgatggtgta caccgggata
      901 acaatttatt cggatgtgcc aatcccacgg ttgtaatggg caatataatt gttagccgat
      961 tcgctaatgg cagggaaaag cgcggcataa atataagccc acacacatgt aacgtactgc
     1021 agatactcgc ttggcagagt tgttacttct tgggaggcag taccagccat cagatattcc
     1081 cgttccatgc caaaacctga gttacccgga taccattaca ttgcctgtat ttcattcatc
     1141 tgccacacac gtgtcagctc tttgtgacct gacgcaatca tctgactcag cagcgattca
     1201 cggtactggt ctttttggtg accgttctga cagagcaata cagtgggaca aacgaccatc
     1261 gtcatcactg gagaggcgtg catggaaact cgtcaacaac tcaagttcgc cggaacggaa
     1321 cgttaacaat agaactttac tgtataaatt tgaatactgc taaatgtatg aatggttcat
     1381 tcatgttaat attgtgattt gcaaaaattt cattttatta gagtaaaata catgtctaaa
     1441 caccaaacac agctttcctt actgcaggac gacatccgca gtcgctatga cagtctcagc
     1501 aaacgtctta aacaagttgc tcagtatatt ctggataaca gtaatagcgt tgcttttgat
     1561 acggttgctt ctattgcgca gcacgcggag gtacctcctt cgacattaat tcgttttgcc
     1621 aatgcgtttg gttttagcgg attcaatgaa atgaaacaag tatttcgcca gcatttgatg
     1681 gaggaaaccg ttagctatac agaacgggct cgtctgttca gacaaaaaaa catcaatgat
     1741 ggggaaccca caccggaaaa accgggcgaa attctgaagc tattttccat ggtaaatgca
     1801 caggtactac aacaattacc gatgcagatt aaaaatgagc aactggatgc tgcagtaagt
     1861 ttattgtcaa aagccgataa tatttatgtg attggattac gtcgttcatt tagcgtggcc
     1921 tgttatttaa cctatgcatt gcgccatctt gagaggcgtg catttttaat tgatggtatt
     1981 ggaggcatgt tttctgagca actgagtatg atcagctcta acgatgtagt catcgccatc
     2041 agttattcgc catacgctca ggaggttgtt gaactggttg agttgggggc aaaacgtgat
     2101 gcacatcata tcgtcattac cgacagtcag gtcagtccgt tggctgcgtt cagtgaggtc
     2161 tgttttgtaa tacgtgaagc gcaggtggat ggcttccgtt cgcaggtagc gtcgatgtgt
     2221 ctggcgcaga cgttggccgt ctcactggct ctggctgctg aataagagga agatgccgat
     2281 aatctgtttt tcatgatcat cggcattttg gctaagctta atctgaagag cgttcagcct
     2341 gaataggttg tggcttgttc ctgcgttttg tcagcacgat atcctccatt ttctctagtg
     2401 agacaccttt ggtttccggc aggtagcggc aaataaagaa atagctaaag acacagcagg
     2461 ctgcgaatat ccacattgga aatgcaccgt ggaaatgggc cagtaaccag ggattttcgt
     2521 taattatagg aaagaactgt gaaaccataa agtttgctat ccacataaat cctacagaaa
     2581 tactcattcc ctgtgaacgc atgcggttgg ggaatatttc tgatatgagc acccatgctc
     2641 caacgcccca ggaaagtgca tagaaaatca taaagaacag cataccaaag agagcataat
     2701 agccggttgt ttggctgtac agagcccatg aagttaataa taaaccaatg atgctaccaa
     2761 tggtgccttt tcgcattagc gataaacgcc ccattttgtc catgatcatt gcgccaataa
     2821 ttgatccgac gagttgaatt acgccaatcc agatggtctg aaacagcgct tcctgggcgc
     2881 taccggtaac atctctcaat acaataggtg cgtaatacat cataacgtta acgccggtta
     2941 cttgctgtaa cattgcgatc atgcaaccga ggaaaaggat aaaacgtacg ttactgtcat
     3001 ggtaattgag cttttggtgg gccgtcagcc ggtcattttg cagagaggtt tttatatctg
     3061 ttagcaaatg tcgtgcatgt tcctcgttag atatctttgt aagaattttt agcgtttctt
     3121 cttcacgacc agccatcatc atccagcggg gggattccgg tataaagaat actaaaatac
     3181 agaacagaat gcatggggca atgccggcag caaacatata acgccagcca atctcaatca
     3241 gccatgtatt ggtcgcaatc gaggcgattt tatagttgac gtaaaatatg aggatctgac
     3301 caaaaacgat ggcaaactgt tgcatactca atgccctgcc acgcatgtcc ttaggtgaaa
     3361 cctccgacat atacattgga gatactgttg ctgcgaggcc gacagcaagt ccacctacga
     3421 tacgataaat aataaagtgc aggaaggtgt aggaaagaga tgttcccact gctgagattg
     3481 tgaacagcag ggcggaaatc atgagtgatt tcttgcgacc aaaacgtttg gataagtaac
     3541 ctgagctgaa tgatcccacc acacaaccta caacgacgct ggaaaccgcc cagccggttt
     3601 cggccggaga taggtgaaaa taactggtga gagaactgat tgcacctgaa ataacggcag
     3661 tatcgtagcc aaataatatc ccgcctaatg ctgcaataga acagatacgt aatatatagc
     3721 ctgtattgta acgattcact gatatgggca tagctgatta tctcctgagc agaaatcatc
     3781 tatctatttg acctataatg aaaaaattgt gtttggtcat ttccgtagct ctgcagttgt
     3841 acttcttgct ttaccatc
//
LOCUS       DAZFLD010000300         3855 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.302, whole genome shotgun sequence.
ACCESSION   DAZFLD010000300 DAZFLD010000000
VERSION     DAZFLD010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3855)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3855)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3855
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.302"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            200..1258
                     /gene="rfaQ"
                     /locus_tag="ACHKBL_004586"
     CDS             200..1258
                     /gene="rfaQ"
                     /locus_tag="ACHKBL_004586"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptosyltransferase
                     RfaQ"
                     /protein_id="HFO5821334.1"
                     /translation="MDKPFRRILLIKMRFHGDMLLTTPVISSLKKNYPDAKIDVLLYQ
                     DTIPILSENPEINALYGIKNKKAKASEKIANFFHLIKVLRANKYDLIVNLTDQWMVAI
                     LVRLLNARVKISQDYHHRQSAFWRKSFTHLVPLQGGNVVESNLSVLTPLGVDSLVKQT
                     TMSYPPASWKRMRRELDHAGVGQNYVVIQPTARQIFKCWDNAKFSAVIDALHARGYEV
                     VLTSGPDKDDLACVNEIAQGCQTPPVTALAGKVTFPELGALIDHAQLFIGVDSAPAHI
                     AAAVNTPLISLFGATDHIFWRPWSNNMIQFWAGDYREMPTRDQRDRNEMYLSVIPAAD
                     VIAAVDKLLPSSTTGTSL"
     gene            1255..2379
                     /locus_tag="ACHKBL_004587"
     CDS             1255..2379
                     /locus_tag="ACHKBL_004587"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFO5821335.1"
                     /translation="MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYTQSWEGE
                     CPDVFELIKVPVKSHTNHGRNAEYFAWVQKHLREHPVDKVVGFNKMPGLDVYYAADVC
                     YAEKVAQEKGFFYRLTSRYRHYAAFERATFEQGKPTQLLMLTDKQIADFQKHYQTEAE
                     RFHILPPGIYPDRKYSQQPANSREIFRKKNGITEQQYLLLQVGSDFTRKGVDRSIEAL
                     ASLPDSLRHNTLLYVVGQDKPRKFEALAEKRGVRSNVHFFSGRNDVSELMAAADLLLH
                     PAYQEAAGIVLLEAITAGLPVLTTAVCGYAHYIVDANCGEAIAEPFRQETLNEILRKA
                     LTQFSLRQAWAENARHYADTQDLYSLPEKAADIITGGLDG"
     gene            2372..3169
                     /gene="rfaP"
                     /locus_tag="ACHKBL_004588"
     CDS             2372..3169
                     /gene="rfaP"
                     /locus_tag="ACHKBL_004588"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HFO5821336.1"
                     /translation="MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAG
                     KSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWNAIHRLRDVGVDTMYGVAFGEKGI
                     NPLTRTSFIITEDLTPTISLEDYCADWATNPPDVRVKRMLIKRVATMVRDMHAAGINH
                     RDCYICHFLLHLPFSGKKEELKISVIDLHRAQLRTRVPRRWRDKDLIGLYFSSMNIGL
                     TQRDIWRFMKVYFVASLKDILKQEQGLLSQAEAKATKIRERTIRKSL"
     gene            3185..>3855
                     /locus_tag="ACHKBL_004589"
     CDS             3185..>3855
                     /locus_tag="ACHKBL_004589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFO5821337.1"
                     /translation="MSAHYFNPQEMINKTIIFDERPAASVASSFHVAYGIDKNFLFGC
                     GVSITSVLLHNNDVSFVFHVFIDDIPEADIQRLAQLAKSYRTCIQIHLVNCERLKALP
                     TTKNWSIAMYFRFVIADYFIDQQDKILYLDADIACQGNLKPLITMDLANNVAAVVTER
                     DANWWSLRGQSLQCNELEKGYFNSGVLLINTLAWAQESVSAKAMSMLADKAIVSRLTY
                     MDQDIL"
BASE COUNT         1009 a          838 c          939 g         1069 t
ORIGIN      
        1 attttatttg attgatttct ttattgaaaa tcaacaatgt gcttaaaaat cattcgggtc
       61 ggattgccgc gaaagaagtg atacactagc gcgtcaatat cagtgcggta tcagtattca
      121 ggtagctgtt gagcctgggg cggtagcgtg ctttttctgc ttcaccttaa tcggataatc
      181 tcaacaaaaa gagttacttg tggataagcc atttcgaaga attttgctca ttaagatgcg
      241 ttttcatggg gatatgttat taactactcc cgtcattagt tcgctgaaaa aaaattaccc
      301 tgacgcaaaa atcgatgtgc tgctttatca ggacaccatc ccgatcctgt ctgaaaatcc
      361 agagattaac gcgctctacg gcataaaaaa taaaaaagca aaagcctcag aaaaaattgc
      421 caactttttc catctcatca aggtattacg tgccaataag tatgacctta tcgttaatct
      481 taccgatcaa tggatggttg ctatactggt gcgcttatta aatgcccgtg tgaaaatttc
      541 ccaggattat catcatcggc agtctgcttt ttggcgtaaa agtttcaccc atttggtgcc
      601 gttgcagggt ggaaatgtgg tggaaagtaa cttatccgtg ctgacaccac tgggagttga
      661 ttcgttggtg aagcagacaa ccatgagtta cccgcctgca agctggaaac gtatgcgtcg
      721 cgaacttgat cacgctggtg ttggacaaaa ttatgtggtt atccaaccta cggcgcggca
      781 aatcttcaaa tgctgggaca acgccaagtt ttccgctgtg attgatgcct tacatgctcg
      841 tggttatgaa gtcgttctga cgtccggccc agataaagac gatctggcct gcgtcaatga
      901 aattgcgcag ggatgccaga cgccaccagt aacggcgctg gccggaaagg tgaccttccc
      961 ggaacttggt gcgttaatcg atcatgcgca gctgtttatt ggcgttgact ccgcaccggc
     1021 gcatattgcc gctgcagtta atacgccgct gatatcgctg tttggtgcga cagaccatat
     1081 tttctggcgt ccctggtcaa ataacatgat tcaattctgg gcgggagatt accgggaaat
     1141 gccaacgcgc gatcagcgtg accgaaatga gatgtatctt tcggttattc cggcggcaga
     1201 tgtcattgct gctgtcgata aattactgcc ctcctccacg acaggtacgt cgttatgatc
     1261 gttgcttttt gtttatataa atattttccc tttggcggtt tgcagcgcga ttttatgcgt
     1321 attgctcaga cagtcgccgc ccgaggtcat catgttcggg tttataccca gtcgtgggaa
     1381 ggcgaatgcc ctgatgtatt tgaactgatc aaagtgccgg ttaaatcgca taccaatcac
     1441 gggcgcaatg cggagtattt tgcctgggtg caaaaacatt tacgcgaaca tcccgtcgat
     1501 aaagtcgttg gattcaacaa aatgccgggg ctggacgttt attatgccgc tgatgtttgt
     1561 tatgccgaga aagtagcgca ggaaaaaggc tttttctatc gcctgacgtc acgttatcgc
     1621 cattatgccg cctttgagcg ggcaaccttc gaacagggca agccgacaca gctgctgatg
     1681 ctgacagata agcaaatcgc cgatttccag aaacattatc agactgaagc ggagcgtttt
     1741 catattctgc caccggggat ttatcctgat cgtaaatata gccagcagcc agctaatagc
     1801 cgtgaaatct tccgtaagaa gaatggaata accgaacaac aatatttatt gttgcaggtc
     1861 ggttcagact tcacgcgtaa aggtgtcgat cgttccattg aagcacttgc ttcgttaccg
     1921 gattcgctgc gccacaacac attgctatat gttgttgggc aggataaacc gcgaaaattt
     1981 gaggcgctgg cagaaaaacg cggcgtgcgc agtaatgttc acttcttctc ggggcgcaac
     2041 gatgtctcgg aattaatggc ggcggcggat ttattactgc atcctgccta ccaggaagcg
     2101 gcgggaattg tgctgctgga agcgattact gcaggattac cggtactaac aactgccgtt
     2161 tgtggctatg cgcattatat tgtcgacgcc aattgcggcg aggctattgc tgagccattc
     2221 cgccaggaaa cattgaatga gattttacgc aaagcgttaa cgcaattttc attgcgccag
     2281 gcctgggcgg aaaatgcgcg acattatgct gatacacaag atttatacag tctgccagag
     2341 aaagcggcgg atatcataac gggtggtctg gatggttgaa cttaaagagc cgtttgccac
     2401 gttatggcgc ggaaaagatc cttttgagga agttaaaact ttgcagggtg aggtatttcg
     2461 tgaactggaa actcgccgta ctctgcgctt tgaaatggcg ggcaaaagct attttctcaa
     2521 atggcatcgc ggcacgaccc tgaaagagat aatcaaaaat ttactctcat tgcggatgcc
     2581 agtattaggc gcagaccgcg aatggaatgc gattcatcga ctgcgggatg tcggcgttga
     2641 tactatgtat ggggtggcat tcggcgaaaa aggcattaat ccgctcacca gaacctcgtt
     2701 tattataacc gaagatctga caccaaccat cagtctggaa gattactgtg ctgactgggc
     2761 gactaaccct ccagatgttc gcgtaaagcg tatgcttatt aagcgtgtcg cgacgatggt
     2821 gcgcgatatg catgctgcgg gcattaacca ccgtgactgt tatatctgtc atttcctgct
     2881 gcacttgcct ttttccggta agaaagagga gttaaaaatt tcggtaattg atctgcaccg
     2941 ggcgcagctt cgcacgcgcg ttccacgtcg ttggcgcgat aaagatctta tagggcttta
     3001 tttttcttcg atgaatattg gcctgactca gcgggatatc tggcggttta tgaaagtgta
     3061 ttttgtcgcc tcgcttaaag acattctcaa gcaggaacaa ggactgctgt cgcaagcaga
     3121 agcaaaagcc acaaaaatca gggaaagaac gattcgaaaa tcgttgtaat ttccggagag
     3181 aaatatgagt gcccactatt ttaatccaca agagatgatc aataagacaa tcatcttcga
     3241 tgaaaggcca gcggcgtcag tggcatcatc attccatgtt gcttatggca ttgataaaaa
     3301 ctttcttttt ggttgtggtg tttcaatcac gtcagttttg ttacataaca acgacgtaag
     3361 ttttgttttc cacgttttta ttgatgatat ccctgaagcc gatatccagc gtttagccca
     3421 attggcgaaa agctatcgta cctgtatcca gatccatcta gtaaattgtg aacggcttaa
     3481 ggcattaccg acgaccaaaa attggtctat tgccatgtat ttccgttttg taattgcaga
     3541 ttactttatt gatcaacaag ataagatcct ttacctggat gctgatatcg cctgtcaggg
     3601 aaacttaaag ccgctgataa caatggatct tgccaataac gttgctgctg ttgttactga
     3661 acgcgatgct aactggtggt cgttacgggg tcaaagtctg cagtgtaatg aacttgaaaa
     3721 gggttacttt aattcaggtg tcctgttaat taatacacta gcgtgggcgc aggagtccgt
     3781 ttctgctaaa gcgatgtcga tgcttgctga taaagccatc gtttcccgtt taacctatat
     3841 ggatcaagat atcct
//
LOCUS       DAZFLD010000301         3825 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.303, whole genome shotgun sequence.
ACCESSION   DAZFLD010000301 DAZFLD010000000
VERSION     DAZFLD010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3825
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.303"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..480
                     /locus_tag="ACHKBL_004590"
     CDS             <1..480
                     /locus_tag="ACHKBL_004590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
                     /protein_id="HFO5821338.1"
                     /translation="PETREFEFANLGFIPLSYYKNRDYACFFSANSTQKPALYDTPDA
                     TANSRINARLPYIFLLSRIAHYLKIIQRENIGTTKDRRLLELELNNWIRGLVTEMTDP
                     GDELQASHPLRDGKVVVEDIEDNPGFFRVKLYAVPHFQVEGMDVSLSLVSQMPKAKA"
     gene            496..1833
                     /gene="tssK"
                     /locus_tag="ACHKBL_004591"
     CDS             496..1833
                     /gene="tssK"
                     /locus_tag="ACHKBL_004591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
                     /protein_id="HFO5821339.1"
                     /translation="MKIYRPLWEDGAFLMPQQFQQQAAWDVHLADSVARMGLAHPWGV
                     VAAEFDDSLLPLSRLNATRLIVRFPDGTLIDTERADNLPPVCDLSTVSERSLVDIVLA
                     LPLLNANGGNLDDGSESERPRRWKSERVNVQELAGHEQSEVAVLRHNLTLRMAHQENA
                     AWLTCPVTRLVRDAQGQWCKDPRFIPPLLTLSASPSLMTELAELLHHLQARRQRLMSM
                     RRENNARLADFAVADVSLFWLLNALNSAEPVLKELLDMPYRHPELLYRELARLAGSLL
                     TFSLEHNVDAVPAYRHETPENVFPPLLSLLNQLLEASLPSRVVFIELKQNGVMWEGAL
                     HDARLREGADFWLSVRSSIPGHELQTKFPQLCKAGSPDDVSEVVNVALSGVIIRPVTH
                     VPAAIPLRLENQYFALDLSTDAARAMLDAGRCTFYTPASLGDVKLELFAVLRT"
     gene            1830..2483
                     /gene="tssL"
                     /locus_tag="ACHKBL_004592"
     CDS             1830..2483
                     /gene="tssL"
                     /locus_tag="ACHKBL_004592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssL, short
                     form"
                     /protein_id="HFO5821340.1"
                     /translation="MNKPVISRAEQIFYPGWLMVSQLRCGQPVEDGKALYRRACQLVT
                     QAREELAEAEFSQESSEIMLYAFCALLDESVLNREKTDDGWHTWQQDPLQAHFFGTLN
                     AGEELWERIREQLKLPAPDVAVLTCLCRTLQLGFTGQYRSQDDERREDVIRALTARVP
                     AFTFAQDAPVVVRAPGYRAGRTMYWLSWVAGIVTLAGLWCVLSSVLADQVARLTGQG"
     gene            2486..>3825
                     /locus_tag="ACHKBL_004593"
     CDS             2486..>3825
                     /locus_tag="ACHKBL_004593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000558483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HFO5821341.1"
                     /translation="MHVRSRLWLTILSLFLTLWLISGFLPLSAVNQIILTLVSVVAAG
                     LVLWYQWYCLSSRRDAPEKAGDDGLPPEYFQGHVLLVAGNSDAWFEPGQAYRETASGW
                     YLRVDTPEQMRILAERLSYSRPALCGQVVVMLGLLPEQHTSDEAFLSWLRNWQRAVMQ
                     CRQWLNTMPPVLVTLMVSGPSPQNTSLNKHSAHWFTLTPDLSGMHIRLTEAGAIPAEE
                     WLRECDAASRLRCVSELLWLNAMLSWYNRVTDALTDICRQEHFYFRPVAVGFCLTSVA
                     ARPGNLWQQQLTSLTVLPPPAGVSSQALPLPDILLSGIPRHRGLSHWHRLCLQTGAIV
                     GVFLLLAMLASFMNNQRLVRSVGEHLTAWHRMRGYSSESKKTALQRLQADSQLLNDWQ
                     QRGVPQRYGLGLYQGMWLIPPVERATGEWIAPLPPRPVIQKVTQTPKMVRLNSLA"
BASE COUNT          756 a          981 c         1154 g          934 t
ORIGIN      
        1 ccggaaactc gggagtttga attcgctaat ctgggcttta ttccgctctc ttactacaag
       61 aaccgggatt atgcctgctt cttctccgct aactccaccc agaaaccggc actgtacgat
      121 acgccggatg caacggcgaa cagccgtatt aatgcccgtc ttccctatat cttcctgctg
      181 tcccgcattg cgcattacct gaagatcatt cagagggaga atatcggtac caccaaagat
      241 cgccgtctgc ttgaactgga actgaataac tggatccggg gtctggtgac ggagatgacc
      301 gatccgggtg atgaactgca ggcctcacat ccgctgcgtg acggaaaagt ggtggtggaa
      361 gacatcgaag acaacccggg atttttccgc gtgaaactct atgctgtacc acatttccag
      421 gtggaaggta tggacgtcag tctgtcactg gtttcgcaga tgccgaaggc aaaagcgtaa
      481 ggcaggcgtc gggaaatgaa gatttatcgt ccactgtggg aagacggggc ttttctgatg
      541 ccccagcagt ttcagcagca ggctgcctgg gatgttcatc tggcagacag cgttgcccga
      601 atggggctgg cgcatccctg gggcgtggtg gctgcagagt ttgatgactc tctgttaccg
      661 ctttcacggt tgaatgccac ccgtctgatt gttcgtttcc cggacggaac gcttattgat
      721 acggaacgtg cggataatct gcctccggtc tgtgatttat cgacggtttc tgagcgttcc
      781 ctggtggata ttgtgctggc gctaccgtta ctgaatgcta acggtggcaa ccttgatgat
      841 ggcagtgaga gcgagcgtcc gcgtcgctgg aaatcagaaa gggtgaatgt gcaggagctg
      901 gccgggcatg agcaaagcga agtggcggta ttgcggcata acctgactct gcgcatggct
      961 catcaggaaa atgctgcctg gctgacctgt cctgttaccc gtcttgttcg agatgcacag
     1021 ggacagtggt gcaaggaccc gcgttttatt ccccctctgc tgacgctgtc tgccagtcct
     1081 tcgctgatga ctgagcttgc ggagttgctt catcacctgc aggcgcgtcg ccagcgactg
     1141 atgtccatgc gtcgggagaa caatgcccgt cttgcggatt ttgcagtggc ggacgtgtcg
     1201 ttgttctggt tgctgaatgc cctgaacagt gcggagcctg ttctgaaaga gttgcttgac
     1261 atgccttacc gccacccgga actgttgtac agggagcttg cccggctggc aggctccctg
     1321 ctcacattct cgcttgagca taatgtggat gcggttccgg cataccgtca tgaaacacct
     1381 gaaaatgtgt ttccaccctt gctttctttg ctgaaccagc tgctggaggc aagcctgcct
     1441 tcccgtgtgg ttttcattga actgaaacaa aatggtgtga tgtgggaggg ggcattacat
     1501 gatgcgcgtt tgcgcgaagg ggcggatttc tggctttcag tgcgctcttc catacccggg
     1561 catgaactgc agacgaagtt tccgcagcta tgcaaagccg gaagccccga tgatgtgtct
     1621 gaggtggtga atgtggcgct gagcggggtc attatccgtc cggttacgca tgtaccggcg
     1681 gccattccgc tccggctgga aaaccagtat ttcgcgctgg atttaagtac ggatgcagcc
     1741 cgggcgatgc tggacgcggg gcgttgtact ttctacaccc cggcatcgct gggggatgtg
     1801 aaactggaac tttttgcggt actgcggaca tgaataaacc tgttatctcc cgggctgaac
     1861 agatttttta tcccggctgg ctgatggtca gccagctgcg ctgcggacaa ccggttgagg
     1921 atggtaaggc cctgtatcgc cgtgcctgtc agctggtgac gcaggcgcgt gaagagctgg
     1981 ctgaagcaga attcagtcag gaaagcagtg aaatcatgtt gtatgccttc tgcgccctgc
     2041 tggacgagag tgtgctgaac cgtgaaaaaa cagacgatgg ctggcacacc tggcagcagg
     2101 acccgctgca ggctcatttt tttggtacgc tcaatgccgg agaagagctc tgggagcgta
     2161 ttcgtgagca actgaaatta cctgcgccgg atgtggcggt actgacatgc ctctgccgta
     2221 cgcttcagct cggttttacc ggtcagtacc ggtcgcagga tgatgagcgt cgcgaagatg
     2281 taattcgcgc actgacagcg cgtgttcctg catttacttt cgcacaggat gcgccggtgg
     2341 tggtgcgtgc accgggttac cgtgctggca gaacgatgta ctggttgtca tgggtggcgg
     2401 gtattgtgac gctggctggt ctctggtgtg tgctttcttc tgtgctcgca gaccaggtgg
     2461 cacggcttac cgggcaggga taaccatgca cgtacgttcc cgtctctggc tgacaatact
     2521 gagtctgttc ctgacactgt ggctgatttc gggattcctg ccactgtctg cagtaaacca
     2581 gataatactg accctggttt cagttgtggc ggcgggactg gtgctgtggt accagtggta
     2641 ttgtctgtct tcccgtcgtg atgccccgga gaaggctggc gatgacggtc tgccgcctga
     2701 atattttcag ggacatgtgc tgcttgttgc cggaaactca gatgcgtggt tcgaacccgg
     2761 tcaggcatac cgggaaacag catcgggatg gtatcttcgt gtggatacgc cggagcagat
     2821 gagaatactg gcagagcggt tgtcgtacag ccgtccggcc ctgtgtggcc aggtcgtggt
     2881 catgctggga cttttaccgg agcagcatac ctcagatgaa gctttcctgt cgtggttgcg
     2941 gaactggcag cgtgcagtca tgcagtgccg gcagtggctg aataccatgc cgccggtgct
     3001 ggtgacattg atggtttcag ggccgtcacc acaaaacaca tccttaaata agcactctgc
     3061 acactggttt acactgacac cggacctgtc gggaatgcac atacgcctga cggaagccgg
     3121 agccattccg gcagaggaat ggctccggga gtgtgatgca gcgtccaggc tccggtgcgt
     3181 cagtgaactg ttatggctga atgccatgtt gtcctggtac aaccgggtta ctgatgccct
     3241 gacagatatt tgccggcagg agcactttta tttccggccg gttgccgttg gattctgtct
     3301 gacgtcggtt gcggcccggc ccggtaatct ctggcaacaa caactgacgt cactcactgt
     3361 actgccaccg cctgccggcg tgtcatcaca ggcactgccg ttgccggata ttctgctgtc
     3421 cggtattccc cgtcatcgtg gactcagtca ctggcacaga ctttgtctcc agacgggggc
     3481 gattgtcggc gtttttcttc tgctggctat gctggcgtct tttatgaata accagcgact
     3541 ggttcgcagc gtgggggaac acctgacggc ttggcaccgg atgcgcggat attcatctga
     3601 atcgaaaaaa actgcactgc aacgtcttca ggctgacagc cagttactga acgactggca
     3661 gcaacgtggt gtgccgcagc gttacggact gggattgtat cagggaatgt ggctgatacc
     3721 gccggttgag cgagcgactg gtgagtggat tgcaccactg cctccccgtc cggtaataca
     3781 gaaagttact cagacgccga aaatggttcg ccttaacagc ctggc
//
LOCUS       DAZFLD010000302         3819 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.304, whole genome shotgun sequence.
ACCESSION   DAZFLD010000302 DAZFLD010000000
VERSION     DAZFLD010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3819
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.304"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..255
                     /locus_tag="ACHKBL_004594"
     CDS             <1..255
                     /locus_tag="ACHKBL_004594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000821923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HFO5821342.1"
                     /translation="RRQEQASARVVDFYRLQFRVARKTLIELLNAESELYSVGLSRVQ
                     TEDQMLHGMLDYLYSQGMLLKWSGVNLSGAEEKVTGQKDR"
     gene            260..2407
                     /locus_tag="ACHKBL_004595"
     CDS             260..2407
                     /locus_tag="ACHKBL_004595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I secretion system permease/ATPase"
                     /protein_id="HFO5821343.1"
                     /translation="MKSIFEGLSQVTALLGTPVSAETLAAGTVRTDVSGIDFRSVGEF
                     LRSEGFDNHLSRRAPEDIPSLAVPVLLLLNAQEAIVVVRIEGAGLARQYHVLQPGGLR
                     KTLSHDELMAVYSGYCWFVQPQATQDRRSELPEYDMTGAWFWRVLWRFRPYYGQVILA
                     SFIINFLALVSSLYVMNVYDRVIPNRSWETLWMLSIGVIAAILFECAAKLIRSYLTDI
                     AGKKADLIISSALLRRVMAVKMSDRPASSGSYASNLREYESVREFMTSATLLAFVDLP
                     FILLFIGVIGLVGGWLAVVPLTLVPLVILAGILLQRPLSRHINASMRESSQRQGLLVE
                     ALDGIETLKLNNAVNWVQRRWDGYTASVARSSIRVKNTSNVIICFSTAVQQLNTVLLV
                     ILGTYLIHSDDPHNRITMGVLIAAVILSGRALAPLGQIASLATRFQQARLAMQGVNDI
                     VRRPVERESQTHYLTVKDIQGEFRFSDVSFSYKPGLPDALCGLSLCIRPGERVGIIGS
                     IGCGKSTLLKMMVGLYRQNRGQITLDGVAMQQLDPCFVRDKVFLLEQNPRLFLGTLRE
                     NLQLSRLPGSLSDVDMINAAGRFGFDRFIRHHPRGLDMQTGEGGQGLSGGQRQMVALT
                     RMVLGSPRVVLLDEPTTGLDLQTESVVLKGLSHWSRGRTMVVVTHRLQVLNIVDRIVV
                     MNEGRVMMDGPRDEILKKLSGKQQSGESRAITS"
     gene            2467..3714
                     /locus_tag="ACHKBL_004596"
     CDS             2467..3714
                     /locus_tag="ACHKBL_004596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001380656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0030253 - protein secretion by the type I
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family type I secretion periplasmic adaptor
                     subunit"
                     /protein_id="HFO5821344.1"
                     /translation="MTEKTERNKASSEVVRQSDLALLTDVQAALQQEKHHALFLMVIF
                     LLVLVVTFVIWAWNSPLDEVTRGQGSIIPGSREQVIQTLDPGILKSLEVREGDIVEKG
                     QVLLTLDDTRSSAMLRESEARVNNLEAVRARLRAEAYSESLTFPDNVPEDLRERESTV
                     YFLRKTELAQSIAGLKQSKALLDREIAMTRPIVREGAMSEVELLRMQRQSAELQLQMD
                     EKQNKYLTEAGAELVKTEAELAQAKENMAGRADPVERSRLRAPLRGIVKNIRVNTLGG
                     VVSAGQDIMEIIPLEDQLLIEAYINPRDVAYVRTGMPALVKLTAYDYAIYGGLDGVVT
                     LVSPDTLRDQKRPGDLKLDPNEAYYRVLVTTSNNYLTDRNGKRLPVIPGMIASVDIKT
                     GRKTVFQYLIKPITRMKQALQER"
BASE COUNT          928 a          812 c         1127 g          952 t
ORIGIN      
        1 agacgtcagg agcaggcctc tgccagagtt gtggactttt atcgtcttca gttcagggtg
       61 gcaagaaaga cactgattga attactgaat gcagaaagcg aactgtacag tgtcgggctt
      121 tcccgggttc agacggagga tcagatgctc cacggaatgc tggattatct gtattcccag
      181 ggaatgctcc tgaaatggag cggagtgaat ctttctggtg cagaagaaaa agtaacaggg
      241 cagaaggaca gataaataag tgaagagtat ttttgaagga ttatcacagg taacggcatt
      301 actgggtaca cctgtatctg ctgagacgct ggctgccggt acagtccgga cagatgtctc
      361 cgggattgat tttcgttctg ttggcgagtt tcttcgcagt gaagggtttg ataatcatct
      421 ttcccggcgg gctccggaag atattccttc acttgcggtt cctgtgcttc ttttactgaa
      481 tgcacaggaa gccattgtgg tggtgcgtat tgagggtgcc ggcctggccc ggcagtatca
      541 tgtactccag cccgggggac tccgtaagac actttctcac gatgaattaa tggctgttta
      601 ttcaggttac tgctggtttg ttcagccaca ggcaacacag gatcgccgtt cggaactgcc
      661 ggagtatgac atgaccgggg catggttctg gcgggtactg tggcggttca ggccatatta
      721 cggacaggtc attcttgcga gttttatcat taactttctt gccctggtca gttccctgta
      781 tgtcatgaac gtttatgacc gggtcatacc caatcgctcc tgggaaacgt tatggatgct
      841 gagtatcggg gtgattgcag ccattctgtt tgagtgtgct gcaaagttaa tccggagcta
      901 tctgacggat attgcgggta agaaggcaga cctgattatc agctcagcac tgttaaggcg
      961 ggtgatggca gtgaaaatgt cggacaggcc ggcgtcttcc ggttcatacg ccagcaattt
     1021 gcgggaatat gagtctgtca gggagtttat gaccagcgcc acgctgctgg cttttgtgga
     1081 tttgccgttt atcctgcttt tcatcggtgt catcgggctt gtggggggct ggcttgctgt
     1141 tgttcctctt acactggttc cgctggtgat tctggcagga atattactgc agcgaccgct
     1201 ttcgcgtcat attaatgcct ccatgcggga atcatcgcag cgccagggat tacttgtgga
     1261 ggcactggac ggaatagaaa ccctgaagct gaataatgca gttaactggg tccagcgccg
     1321 ctgggatgga tatacagcca gtgtcgcccg ctcgtccata cgggtaaaaa ataccagtaa
     1381 tgtcataatc tgcttttcca cagcagttca gcaactgaat actgttctgc tggtcattct
     1441 ggggacgtac cttattcaca gtgatgaccc acataaccga atcaccatgg gggtactgat
     1501 tgccgcagtt attctttccg gtcgtgcgct ggcaccgctt ggtcagatag catcactggc
     1561 cacccgtttt cagcaggccc gcctggccat gcagggggtg aatgacattg tccgccgtcc
     1621 ggtggagcgg gaaagtcaga cacattatct gacagtgaag gatatccagg gagagtttcg
     1681 tttcagtgat gtctcctttt cttataagcc cggattaccc gacgcattgt gcggtctgtc
     1741 gctctgtatc cggcccggag agcgggtggg tattattggc tcaataggtt gtggtaaatc
     1801 gacgctgctg aaaatgatgg tggggctgta ccgtcagaac aggggacaaa ttactcttga
     1861 cggggttgcg atgcagcaac tggacccctg ttttgtccgg gataaagtgt ttcttctgga
     1921 acagaatccc cggctttttc tgggaactct gcgtgaaaat ctgcagcttt cgcgtctgcc
     1981 gggaagcctg tcggatgtgg atatgattaa tgcagccgga cgtttcgggt ttgaccgttt
     2041 tatccggcat catccccgcg gacttgatat gcagacaggg gaaggaggtc aggggctgtc
     2101 aggaggacag aggcagatgg ttgccctgac gagaatggta ctgggttctc cccgggtggt
     2161 actgcttgat gagcccacga caggtctgga cctgcagact gagtctgttg ttctgaaagg
     2221 tctgagccac tggagccgtg gacgcacaat ggtggtggtg acacatcgtc tgcaggttct
     2281 gaatattgtt gaccgtattg tcgtgatgaa tgaagggcgg gtaatgatgg acggtccccg
     2341 tgatgaaata ctgaaaaaac tgtcagggaa acagcagagt ggggagagca gagcgataac
     2401 ttcatgagcg tcaaccggat aagaaaaaag agcagatatg tttaacagtt tgtggaccgc
     2461 agggatatga cagaaaaaac ggaacggaat aaagcgtcgt cagaggtggt caggcagtca
     2521 gacctggcgc tgttgacaga tgtacaggca gcccttcagc aggaaaagca tcatgccctt
     2581 ttcctgatgg tgatttttct gctggtactg gtagtgacgt ttgttatctg ggcatggaac
     2641 agtcctctgg atgaagtgac ccgaggtcag gggagtatta ttcccggcag cagggagcag
     2701 gttatacaga cccttgaccc gggtatactg aaatcacttg aggtcaggga aggcgatatt
     2761 gttgagaaag gtcaggtact gctgacactg gatgataccc ggagctctgc catgctgagg
     2821 gaaagtgaag cccgggtgaa taacctggag gccgtcaggg caagactccg tgcagaagct
     2881 tattccgaat cactgacatt tccggataac gttcctgagg atttgcggga gcgggaaagt
     2941 acggtctatt ttctccggaa gacggagctg gcgcagagca ttgccggtct gaagcagagt
     3001 aaggcgctgc tggacaggga aatagcaatg acgcggccaa ttgtgcgtga aggggcaatg
     3061 tcggaggtcg aactgttacg tatgcagcgt cagtcagcgg agctgcagtt acagatggat
     3121 gagaaacaga ataaatacct gacggaagcc ggtgctgaac tggtgaagac agaagcagag
     3181 cttgctcagg caaaagagaa tatggctggc agagccgacc cggttgagcg ttcacgtctc
     3241 agggcccccc tgagaggtat cgtaaaaaat atacgggtaa acacactggg aggggtggtt
     3301 tctgcaggac aggacataat ggaaattatt ccgctggaag accagttgct gattgaagct
     3361 tatataaatc cacgggatgt tgcctatgtc agaacaggga tgccggcact tgtaaaactg
     3421 acggcttatg actatgccat ttatggtggt cttgacggcg tggtgacact ggtaagtccg
     3481 gatactttac gtgaccagaa acggccggga gatttaaaac tggacccgaa tgaagcttat
     3541 taccgggtac tggttaccac cagtaataat tatctgacag acaggaacgg aaaaagattg
     3601 ccagttattc ctggcatgat agccagcgtg gatataaaaa ccgggcgtaa aacggtattt
     3661 cagtatctga ttaaaccgat aaccagaatg aagcaggcac ttcaggaaag ataataaaaa
     3721 ggtcttccgg ggaaagtatt ttaaatcaat tttaaacggg cagaatgatt tcactttatt
     3781 ctgcccgggt cattgcgcat gcctgtatgt gatttgggg
//
LOCUS       DAZFLD010000303         3795 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.305, whole genome shotgun sequence.
ACCESSION   DAZFLD010000303 DAZFLD010000000
VERSION     DAZFLD010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3795
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.305"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..615
                     /locus_tag="ACHKBL_004597"
     CDS             <1..615
                     /locus_tag="ACHKBL_004597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000077650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbC"
                     /protein_id="HFO5821345.1"
                     /translation="GEFFTVFQGDVVPSASVYIPDSEKSCDSDPVVINRYISVEAPRL
                     ERLRRLVPRTVQRRLPTPEHVSIIGVLTAKPSRKRRKNRTEPYRIIDTFQHRLATAQT
                     SLDLLPQYDTDIESRANELWKKAVDTINNTTREERRVCYITLNRPEEEHSGPEDIPSV
                     KAILNTLLPLEILLPVAENNKSQTINNDHPQKSCGAQPTTDEKY"
     gene            complement(836..3556)
                     /locus_tag="ACHKBL_004598"
     CDS             complement(836..3556)
                     /locus_tag="ACHKBL_004598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000991381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFO5821346.1"
                     /translation="MNVIIPHKRRDGRSSFEDLIAYTSVRDDVQENELTEDSEIKSDV
                     PHRNRFRRLVDYATRLRNEKFVSLIDVMKDGSQWVNFYGVTCFHNCNSLETAAEEMQY
                     KADKAVFSRNDTDPVFHYILSWPAHESPRPEQLFDCVRHTLKSLELSKHQYVAAVHTD
                     TDNLHVHVAVNRVHPETGYINWLSWSQEKLSRACRELELKHGFAPDNGCFVHAPGNRI
                     VRKTALVRERRNAWRRGKKQTFREYIAQMSIAGLREEPAQDWLSLHKRLASDGLYITM
                     QEGELVVKDGWDRAREGVALSSFGPSWTAEKLGRKLGEYQPVPTDIFSQVGTPGRYDP
                     EAINVDIRPEKVAETESLKQYACRHFAERLPAMARNGELKSCLAVHRTLAEAGLWMGI
                     QHGHLVLHDGFDKQQTPVRADSVWPLMTLDYMQDLDGGWQPVPKDIFTQVVPGERFRG
                     HSIGSQPVSDYEWYRMRMGTGPQGAIKRELFSDKESLWGYALIQCRSQIEEMIAGGDF
                     SWQACHEMFARKGLMLQKQHHGLVIVDAFNHELTPVKASSIHPDLTLSRAEPQAGPFE
                     IAAADIFERVKPECRYNPELAASDEVEPGFRRDPVLRRERREARAAAREDLRARYLAW
                     KEHWRKPDLRYGERLREIHAACRRRKAYIRVQFRDPQLRKLHYHIAEVQRMQALIRLK
                     ESVKEERLSLIAEGKWYPLSYRQWVEQQAAQGDRAAVSQLRGWDYRDRRSRNKDKRRT
                     TNADRCVVLCEPGGTPLFNNVAKLEARLQKNGSVHFRDTRTGKNVCTDYGDRVVFYHH
                     TDRNELAEKLDLIAPVLFSRNGKLGFEPEGSYQQFNDVFAEMVAWHNAAGITGNGHFT
                     ITRPDVDLHRQRSEQYYREYIRQQTRLSESHDDNYTLRQEKTWEPPSPGM"
     gene            complement(3568..>3795)
                     /gene="mobA"
                     /locus_tag="ACHKBL_004599"
     CDS             complement(3568..>3795)
                     /gene="mobA"
                     /locus_tag="ACHKBL_004599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization protein MobA"
                     /protein_id="HFO5821347.1"
                     /translation="ESSGQTVSTFIRSSSLDKVVNCRTDDRMIDEIMRLGRLQKHLFV
                     EGKRTGDKEYAEVLVAITEYVNALRRDLMGR"
BASE COUNT          932 a         1088 c          835 g          940 t
ORIGIN      
        1 ggtgagttct ttaccgtttt ccagggggat gtggtgccgt cagcctctgt gtatattccg
       61 gacagcgaga aaagttgcga cagtgatccg gtggtcatca accgctatat ttccgttgaa
      121 gccccccggc tggagcgtct ccgccgtctc gtccccagga cagtgcagcg caggctcccc
      181 acaccggagc atgtcagcat catcggtgta ctgacagcaa aaccatcccg taaacgtcgt
      241 aaaaaccgta cagagcctta ccgtatcatt gatacattcc agcaccggtt ggcaacagcg
      301 cagacatcac tggatctgct tccgcaatat gatacagata tcgaatcccg ggcaaacgaa
      361 ctgtggaaaa aggctgtgga cactatcaat aacacaacca gggaagaacg ccgggtatgt
      421 tatatcacac tgaatcgtcc ggaagaggaa cactccggtc ctgaagatat accttcagtg
      481 aaggcgattc tgaataccct gctccccctg gagatactgt taccggtcgc tgaaaataat
      541 aaatcgcaga caataaataa tgatcatccc cagaaatcat gtggagcaca accaacaact
      601 gacgaaaaat actgaaggaa tacaaatatt ttctggtgac catatctgtc aatcagactg
      661 taaaaccctg ttcactaagt ccggggtttt acggcgcact ggataagggc cgcagacacg
      721 gcccttaatt cagagttaac cgggtataaa ctcaattcag tgactactcc cgcccttatt
      781 gacgaaggtt cctgccgttt ttgggctgaa tctttgcacc atcacttatc cgggcttaca
      841 ttccaggtga tggtggctcc catgttttct cctgccgtaa cgtataatta tcatcatggc
      901 tctccgataa acgggtctgc tgtctgatat attcccggta atactgctca ctgcgttgac
      961 ggtgcaaatc cacgtccgga cgagtaatgg tgaagtgtcc gtttccggtt attcccgctg
     1021 cattgtgcca ggccaccatt tctgcaaaca catcattaaa ctgctggtat gaaccttccg
     1081 gctcaaaacc aagctttccg ttgcggctga acagaactgg cgcaattaaa tcaagcttct
     1141 ctgccagttc atttcggtcc gtgtgatggt agaacacaac ccgatctccg taatccgtac
     1201 agacattttt tcctgtccgc gtatccctga aatgaacact tccatttttc tgaagacgag
     1261 cttcaagttt tgcaacatta ttaaaaagtg gcgttcctcc gggttcgcag agtacgacac
     1321 aacgatcagc attagttgtg cgtcttttat ccttattacg cgagcgacga tcacgataat
     1381 cccagccacg taactgcgat actgcagccc tgtctccctg cgccgcctgt tgttccaccc
     1441 actgccggta ggacaacgga taccacttac cctccgcaat cagtgacaac cgctcttcct
     1501 tcacactctc cttcagcctg atcagcgcct gcatacgctg cacttccgca atatggtaat
     1561 gcagcttacg caattgtgga tcccggaact gcacacggat atacgccttg cgccgacggc
     1621 aggcagcatg aatttcccgt aaccgttctc cgtaacgtaa gtcgggttta cgccagtgct
     1681 ccttccatgc aagataacgc gcacgcaaat cttcccgcgc agctgcacgg gcttcacggc
     1741 gctcacgacg cagtacagga tcgcgccgga agccgggctc cacttcatca ctggcagcaa
     1801 gttccggatt gtaacgacat tcaggcttca cccgttcgaa aatatctgca gccgcaattt
     1861 caaatggccc ggcctgaggc tcggcacggg ataacgtcaa atccgggtgg atactgctgg
     1921 ccttcaccgg tgtcagctca tgattaaacg catccacaat cacaagacca tgatgctgtt
     1981 tctgcagcat aagcccttta cgcgcaaaca tctcatgaca tgcctgccag ctgaaatccc
     2041 cgccagctat catctcttca atctggctcc tgcactgaat aagagcgtat ccccacagac
     2101 tttccttatc ggaaaacagt tcacgcttta tagccccctg cggacctgtg cccatcctca
     2161 tgcgatacca ttcgtaatca ctgacaggct gagagccaat actgtgtccc cggaatcgtt
     2221 ctccgggaac gacctgagta aaaatatcct tcggtacagg ctgccatcca ccatccagat
     2281 cctgcatgta atccagcgtc attaacggcc acacactgtc tgcacgtact ggtgtctgtt
     2341 gtttatcaaa tccatcatgc agtaccagat gcccgtgctg aatccccatc cataatcccg
     2401 cctctgccag agtacggtgt acagccaggc agcttttcag ttcaccattg cgcgccattg
     2461 ccggtaagcg ttcggcaaaa tgacggcagg cgtactgttt cagactctcc gtttctgcca
     2521 ctttttccgg ccggatatcc acgtttatgg cttccggatc ataacgaccg ggtgtcccca
     2581 cctgactgaa aatatctgtc ggtacaggct gatattcccc cagtttccgc cccagttttt
     2641 cagctgtcca tgacggtccg aacgaactga gtgcaacacc ttcccgcgct ctgtcccagc
     2701 catccttcac caccagttcc ccttcctgca tcgtgatata cagaccatca ctggccagcc
     2761 gtttatgcaa agacaaccag tcttgtgcgg gctcttcacg cagcccggca atcgacatct
     2821 gcgcaatata ttccctgaac gtctgctttt tcccgcgtcg ccaggcatta cggcgctcac
     2881 gtaccagcgc tgttttgcgc acaatacggt tgccgggtgc atgcacaaag cagccattat
     2941 ccggcgcgaa tccgtgctta agttcaagtt cacggcatgc acggctgagt ttttcctggc
     3001 tccaggaaag ccagttaata taaccagttt caggatgtac acgattcacg gcaacatgca
     3061 catgcagatt atccgtgtcc gtatgaacgg cagccacgta ctgatgtttt gagagttcaa
     3121 gagatttcag tgtgtgacgc acacagtcaa aaagctgctc aggacgcggg ctttcatgcg
     3181 caggccagga aagaatgtag tggaaaacgg ggtctgtatc attacgggaa aaaacagctt
     3241 tgtccgcctt gtactgcatc tcttcagcag ctgtttccag tgaattgcag ttatggaaac
     3301 aggttacgcc atagaaattt acccactgac tgccgtcttt catcacatca atcagggaga
     3361 caaatttttc gttacgcagg cgcgttgcat aatcaacaag ccgtctgaaa cggttgcggt
     3421 gagggacatc actcttgatt tcgctgtctt ctgtgagctc attttcctgc acatcatccc
     3481 gcacagacgt gtacgcaatc aggtcttcaa aggaagacct gccgtcacgt cttttgtgcg
     3541 gaataatgac attcatcagc agttatctca gcgtcccatt aagtctcttc gcagagcatt
     3601 gacatattca gtaatggcaa caagaacctc tgcatactcc ttatcaccag tgcgttttcc
     3661 ctccacaaac agatgcttct gaaggcggcc aagacgcata atctcatcaa tcatacggtc
     3721 gtcagtacgg cagttaacca ccttatccag tgaagagctt cgtataaatg ttgaaaccgt
     3781 ctgtccactt gactc
//
LOCUS       DAZFLD010000304         3758 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.306, whole genome shotgun sequence.
ACCESSION   DAZFLD010000304 DAZFLD010000000
VERSION     DAZFLD010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3758
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.306"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..536
                     /locus_tag="ACHKBL_004600"
     CDS             <1..536
                     /locus_tag="ACHKBL_004600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5821348.1"
                     /translation="NIKVSYSLKINQTTPGILVSCDSNNNFACQTTMTEDVIQRITTF
                     FQTRPDVKNREIKLEWSGDKRDLPTAEAEISRVQASIIKWYASEYHNGRQVLDEIQTP
                     SAINSELYTKMIYLTRNWSLYPNGDGCVTISSPEIKSKYPAAICLILGFFLSIVISVM
                     FCLVKKMVDEYQQNSGQ"
     gene            complement(777..1472)
                     /locus_tag="ACHKBL_004601"
     CDS             complement(777..1472)
                     /locus_tag="ACHKBL_004601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HFO5821349.1"
                     /translation="MDNRSEFLNNVAQALGRPLRLEPQAEDAPLNNYANERFTQLTQQ
                     QRCDAFIQFASDVMLTRCELTSEAKAAEAAIRLCKELGDQSVVISGDTRLEELGISER
                     LQQECNAVVWDPAKGIENISQAEQAKVGVVYAEYGLTESGGVVLFSAAGRGRSLSLLP
                     ESSLFILRKSTILPRVAQLAEKLHQKAQAGERMPSCINIISGPSSTADIELIKVVGVH
                     GPVKAVYLIIEDC"
     gene            complement(1465..2892)
                     /locus_tag="ACHKBL_004602"
     CDS             complement(1465..2892)
                     /locus_tag="ACHKBL_004602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LutB/LldF family L-lactate oxidation iron-sulfur
                     protein"
                     /protein_id="HFO5821350.1"
                     /translation="MSIKTSNTDFKTRIRQQIEDPIMRKAVANAQQRIGANRQKMVDE
                     LGHWEEWRDRAAQIRDHVLSNLDAYLYQLSEKVTQNGGHVYFAKTKEDATRYILQVAQ
                     RKNARKVVKSKSMVTEEIGVNHVLQDAGIQVIETDLGEYILQLDQDPPSHVVVPAIHK
                     DRHQIRRVLHERLGYEGPETPEAMTLFIRQKIREDFLSAEIGITGCNFAVAETGSVCL
                     VTNEGNARMCTTLPKTHIAVMGMERIAPTFAEVDVLITMLARSAVGARLTGYNTWLTG
                     PREAGHVDGPEEFHLVIVDNGRSEVLASEFRDVLRCIRCGACMNTCPAYRHIGGHGYG
                     SIYPGPIGAVISPLLGGYKDFKDLPYACSLCTACDSVCPVRIPLSKLILRHRRVMAEK
                     GITAKAEQRAIKMFAYANSHPGLWKVGMMAGAHAASWFINGGKTPLKFGAISDWMEAR
                     DLPEADGESFRSWFKKHQAQEKKNG"
     gene            complement(2903..3622)
                     /locus_tag="ACHKBL_004603"
     CDS             complement(2903..3622)
                     /locus_tag="ACHKBL_004603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HFO5821351.1"
                     /translation="MNVNFFVTCIGDALKSRMARDSVLLLEKLGCRVNFPEKQGCCGQ
                     PAINSGYIKEAIPGMKNLIAALEDNDDPIISPAGSCTYAVKSYPTYLADEPEWASRAE
                     KVAARMQDLTSFIVNTLGVVDVGASLQGRAVYHPSCSLARKLGVKDEPLTLLKNVRGL
                     ELLTFAEQDTCCGFGGTFSVKMAEISGEMVKEKVAHLMEVRPEYLIGADVSCLLNISG
                     RLQREGLKVKVMHIAEVLMSR"
BASE COUNT          979 a         1003 c          801 g          975 t
ORIGIN      
        1 ataatataaa agtgagttac agtctaaaaa taaaccagac aacaccaggc atacttgtta
       61 gctgtgatag caataataat tttgcctgtc agaccacaat gactgaagat gttattcagc
      121 gaattactac attttttcag accagaccag atgtcaagaa cagagaaata aagctggaat
      181 ggtcaggaga taagagagat ttaccaactg ctgaagcgga aatatctcgc gtgcaggcgt
      241 ctattatcaa atggtatgcg tcagaatacc ataatggcag gcaagttctt gatgagatac
      301 aaacgccttc agcaattaac agtgagcttt atacaaaaat gatatacctg accaggaact
      361 ggtcattgta tccgaacggt gatggctgtg taaccataag ctcaccggaa ataaaaagta
      421 aataccctgc tgccatttgt ctgattctgg gattttttct aagcattgtg atttctgtaa
      481 tgttttgcct tgtcaaaaaa atggtagatg aataccaaca aaactctggg cagtaataaa
      541 cagcattctg acagagagtg cctgtataaa actatagtga caatgaccgg gaagggagcc
      601 cgtcttcagg aatggattcc tgtctttttc actgtaaata tcctggcatt attaatgtca
      661 ggatatttac agaagagcag cataaccatg acggaacgga gttaagtcag gttgtcatta
      721 gcgctattcg caacctcgtg ccgccttcag gtttatgtcc agacttcata tctctctcag
      781 caatcctcaa taatcagata caccgctttc accggaccat gaaccccgac gactttgata
      841 agctcaatgt ccgccgttga actgggacca ctgatgatgt taatacatga cggcattcgt
      901 tcaccggcct gcgctttctg atgcaatttt tctgcgagtt gcgctacacg cggcaggatt
      961 gtgcttttac gcaggataaa aagagaagat tccgggagca ggctcaatga acgcccgcgc
     1021 ccggcggcgg aaaaaagaac cacgcctccc gattcggtta aaccatattc agcatatacg
     1081 acgcccactt tagcctgctc tgcttgtgat atgttctcaa tacctttcgc cggatcccaa
     1141 acaacggcat tgcattcctg ctgcaaacgt tcgctaatcc ccaattcctc caacctcgtg
     1201 tcaccactaa tcacgaccga ctgatctccc agctctttac acagacgtat tgcagcttct
     1261 gccgccttcg cctcgctggt cagttcacag cgcgtcaaca taacatcgct ggcaaactgg
     1321 ataaacgcgt cacagcgctg ctgttgggta agttgggtaa accgctcgtt agcatagttg
     1381 ttaagcggcg catcttctgc ttgcggttca agtcgcagcg ggcgacccag tgcctgagca
     1441 acgttattca aaaattcgct ccgattatcc attctttttc tcctgcgcct gatgtttctt
     1501 aaaccaacta cggaaactct ctccgtcagc ttcaggaaga tcgcgtgctt ccatccagtc
     1561 gctaatcgcg ccaaatttga gtggtgtttt gccgccattg ataaaccagc ttgccgcatg
     1621 agcaccagcc atcatcccaa ctttccacaa tcctggatga ctattggcat aagcgaacat
     1681 ttttatcgcc cgttgctctg cttttgcggt gatccctttt tcagccatca cccgacgatg
     1741 acgcaaaatc agttttgaca gcggaatacg caccggacac acgctgtcac aagcggtgca
     1801 taaagagcag gcgtagggta aatctttaaa atctttatag ccgccaagta acggggaaat
     1861 caccgcacca attggccctg gataaataga gccatatcca tgaccgccaa tatggcgata
     1921 tgccggacaa gtattcatac aagccccgca acgaatacag cgcagcacat cccgaaattc
     1981 agaggccagc acctcagaac gcccgttatc gacaataacc agatgaaact cttcaggacc
     2041 atcaacgtgc cccgcttcgc gcggtcctgt cagccaggtg ttgtatcccg tcaaacgtgc
     2101 accaacggca ctgcgcgcca gcatggtgat caatacatct acctctgcaa acgtgggagc
     2161 aatacgctcc attcccatca ctgcaatatg cgttttaggc agcgtggtac acattcgcgc
     2221 attaccttca ttggtcacca ggcataccga accggtctct gccaccgcga aattacagcc
     2281 ggtaatacct atttcagcac tgaggaaatc ttcgcggatt ttttgccgaa tgaataaggt
     2341 catcgcttca ggcgtttccg gcccctcata gcccagacgt tcgtgtagca cccgacggat
     2401 ctgatggcga tctttatgaa ttgccgggac cacaacatga gagggcggat cttgatccag
     2461 ctggagaatg tattcaccca gatcggtttc aatcacctga atgccagcat cctgcaacac
     2521 atgattgaca ccaatctctt cggtcaccat cgatttagat ttcaccacct tccgggcatt
     2581 tttgcgttgg gcaacctgta aaatgtagcg ggtagcgtct tctttggttt ttgcaaaata
     2641 gacgtgaccg ccgttttgcg tcactttttc tgagagctgg tacagataag cgtcgagatt
     2701 actcagaaca tgatcacgta tctgggcggc ccgatcgcgc cactcctccc agtgccccaa
     2761 ttcatcgacc attttttgcc gatttgcccc gatacgctgc tgcgcgtttg ccaccgcttt
     2821 gcgcatgatc ggatcttcaa tttgctgacg gatgcgtgtc ttaaaatctg tattactggt
     2881 tttgatcgac atctttatat cctcagcggc tcatcaacac ttcagcaata tgcatcactt
     2941 tgactttcag cccttcccgt tgtaatcgtc cactgatgtt cagcaggcaa ctcacgtcag
     3001 cacctattaa atactcaggg cggacttcca tcaggtgcgc aaccttttct ttcaccatct
     3061 cgccggatat ttcggccatt ttgaccgaga acgtgccgcc aaatccgcag caggtatcct
     3121 gttcagcaaa ggtcaacagc tccagtccac gcacattttt cagcagcgta agtggttcgt
     3181 ccttcacccc cagcttacgg gccaggctac aagatgggtg atacaccgct ctcccttgca
     3241 aactggcacc tacatcgact acccctaatg tattaacaat aaaagaggtg agatcctgca
     3301 tacgcgcggc aaccttttcg gcacgtgatg cccattcagg ttcatccgcc agatacgtcg
     3361 ggtaactttt tacggcatag gtgcaagagc cagctggtga aataatggga tcgtcgttat
     3421 cctccagtgc ggcgatcaga tttttcatcc ctggaatcgc ttctttgata taaccactat
     3481 tgatcgcagg ctgaccgcag catccctgtt tctccgggaa atttacgcga cagccgagtt
     3541 tttccagtag cagcacggag tctcgtgcca ttcttgattt cagggcgtca ccaatacagg
     3601 tgacaaagaa attgacattc acaactaata ctccattact tcatgcccat ttatgcccga
     3661 tggttccccc cttccggggg atcaatcatc aataaatatg gcgttctttt ttaattggct
     3721 agcaacatac cacccctcat ttgtatgaca aatgataa
//
LOCUS       DAZFLD010000305         3753 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.307, whole genome shotgun sequence.
ACCESSION   DAZFLD010000305 DAZFLD010000000
VERSION     DAZFLD010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3753
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.307"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..213)
                     /locus_tag="ACHKBL_004604"
     CDS             complement(<1..213)
                     /locus_tag="ACHKBL_004604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5821352.1"
                     /translation="MKIKQHKEKLEIIRNIIRETLSGNAAIVVLVSMIFVMLEKPWSV
                     MSVLCIFVYCISLIVVFLWWFFIVFIY"
     gene            476..1300
                     /locus_tag="ACHKBL_004605"
     CDS             476..1300
                     /locus_tag="ACHKBL_004605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001532113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HFO5821353.1"
                     /translation="MKTDSTGIAARMMLSLDRERICECLLSNRQLQSTPLQVRYPQGI
                     REALGIMSEQLSLSVSDLTRILVEDALSEMFLPADNIVRRLLSRMEHIMQAHDISATT
                     MAALLAPWNIRPAVFREPDRLTDYLTGEILAALADWFYLSPEWLNGRVHYPLYHPGDW
                     PITQHDFHRLISDSENIDIILWHGFPFAGMHSQEYCGVLLRQKKNINNAIIHPVLSLY
                     PVRMDKEKEGWFQMIRKTSPDIPARAVTLTPAQAEFLITGKILPSVLFRAPLSPWK"
     gene            1399..2598
                     /locus_tag="ACHKBL_004606"
     CDS             1399..2598
                     /locus_tag="ACHKBL_004606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001532112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraF"
                     /protein_id="HFO5821354.1"
                     /translation="MMMKKHITRTLIASAVLFSFNSTAATSYFEARNDAMGGTGVASS
                     HYGVAPLANPALLTKSGAKDDFSLLLPSVGAQLSDPGNIRDNADKISDDWKAFDRAVD
                     SQSGVTQAAARLKARLQDFRHTHADAQVGASAVAALPGDTLAGALMLKSYGTVSVDGK
                     VSDADLNYLESIASSSQSVDKDRLTSQAFARAALITDVGIALATELETAGQKWSLGFT
                     PKFQRVDLFNYNVLVKNYDSSDFKGDRYHNTRNGINADIGASMDLDDNWTLGLVAQNL
                     IPRSIETKEVNGITETFRIRPQVTAGVSWHNDMFTTALDVDLTPASGFTSDSKRQFAA
                     IGAEFNARKWVQLRAGYRQNLASNDGSAFTAGVGVSPFDVVHLDLAGLAGTDNNYGAI
                     AQLQFTF"
     gene            2703..3161
                     /locus_tag="ACHKBL_004607"
     CDS             2703..3161
                     /locus_tag="ACHKBL_004607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001532111.1"
                     /note="Members of this family include DotD of type IVB
                     secretion systems and TraH of plasmid conjugative plasmid
                     systems, both lipoproteins; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DotD/TraH family lipoprotein"
                     /protein_id="HFO5821355.1"
                     /translation="MPKKHITLAGGIVVLSLSGCHLPPDNTAPVMKYGAASEVAVTRH
                     AQYQLWEQGTYSEASPSRQAGGSLQAHSGRVSFDWEGDALELLSELARLRGQQFSYSG
                     VRLPLPVSLHVRDMTFTDVLRLTEAQTAWRAALTQSAGRLHLSYMPTEKK"
     gene            3158..>3753
                     /locus_tag="ACHKBL_004608"
     CDS             3158..>3753
                     /locus_tag="ACHKBL_004608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001672079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HFO5821356.1"
                     /translation="MKKQVCLLLSGLLLAGCTGQHHHSSAVVTENAPPPDIRAWLTPS
                     GNQDDGISDTRRQMLTDAGRTLGFRGGKAQRSWELIQALNARESTLNALYDFRPLISK
                     EGWLPPVIDEAQDVAHIRPDQIRTASRVWTILRPERFVSNPPGWRDWLLKGLSTTATP
                     GTEGRVVPEDRAQRRLWENALRQGWQEGRLNADLTLEA"
BASE COUNT          953 a         1050 c          903 g          847 t
ORIGIN      
        1 ataaataaaa acaataaaaa accaccacag aaaaacaact attaaagaga tacagtaaac
       61 aaatatacac aacacactca tcaccgacca gggtttctcc agcatgacaa aaatcattga
      121 taccagcacc acaatagctg cattacctga cagagtttca cgaataatat tccttataat
      181 ttccagtttt tccttatgct gttttatttt catactcacc tcattgattt tatcccgaac
      241 agaaaacgat tatataacga aatactttca ctcttatgag tacggctact ctgcagcata
      301 atgccggacc gacagcatac ctggtcagtc cctgctcctg actcccttgc aaagagtgat
      361 taacactctt ttaaagcact cgatcataac gatcgaaata actcgtttcc gtttaaaaaa
      421 cacactcttt agactcaatt tatgaacaca acgcgctgcc ccgcacggag gtgaaatgaa
      481 aactgacagc acaggtattg ccgcccgtat gatgctctcc ctggacaggg agcgaatttg
      541 cgaatgcctg ctcagtaatc gccagctgca gtccacgccc ctgcaggttc gttatccgca
      601 ggggatcagg gaggctctcg gcatcatgag cgaacagctt tccctttccg tctcagacct
      661 gacgcgtatc ctggtcgaag acgcgttaag tgaaatgttt ctccctgcag ataacatcgt
      721 ccgtcgtctt ctcagcagga tggagcacat catgcaggca catgatatca gcgcaacaac
      781 gatggccgca ttactggctc catggaatat ccgtcctgcc gtctttcggg agccggacag
      841 actcactgac tatctgaccg gggaaattct tgcagcactg gctgactggt tttatctcag
      901 tccggaatgg ctgaacggca gagtgcatta tccgctgtat catccggggg actggccgat
      961 cacacagcac gatttccacc ggcttatatc agacagcgag aatatagaca ttattctctg
     1021 gcatggcttt ccttttgccg ggatgcattc tcaggaatat tgcggtgttc tgctcagaca
     1081 gaaaaaaaac attaacaatg ccattattca tcccgtgctc tcgttatatc cggtcaggat
     1141 ggataaggaa aaagaaggct ggtttcagat gatacgaaaa acatctccgg atatccctgc
     1201 acgcgccgtc acgctcactc cggcacaggc agaatttctg ataacaggga aaatattgcc
     1261 ctctgtatta ttcagagctc cgttatcacc ctggaaataa aactgaagaa taaaccgaaa
     1321 taataatcca actgttttta atggcacaga cgtgcccact gttgtcatac ccaaaatacc
     1381 tcaatacagg aaactgaaat gatgatgaaa aagcatatta cccgtacact catcgcatct
     1441 gccgttttat tttcctttaa ttcaactgca gcaacttcat attttgaagc ccgcaatgat
     1501 gcaatgggtg gaaccggggt ggcctcctct cactacggcg tggcaccact ggcaaacccg
     1561 gccctgctga caaaatccgg tgcaaaagat gacttcagtc ttctgcttcc gtccgtcggg
     1621 gcgcaattgt ccgacccggg taatatcaga gacaacgctg acaaaatcag tgatgactgg
     1681 aaagcctttg atcgtgccgt cgacagtcag agcggcgtga cacaggccgc tgcgcgtctg
     1741 aaagcgcgac ttcaggactt ccgtcacacg catgctgacg cacaggtggg cgcttccgct
     1801 gtggcagcgc ttcccggtga cactctcgcc ggcgctctga tgctgaaaag ctacggtacg
     1861 gtcagtgtcg acggtaaagt cagtgatgcc gaccttaact acctggaaag cattgccagc
     1921 agtagccagt ctgtcgataa agacaggctg acctctcagg cttttgcccg tgctgcactg
     1981 atcactgatg tgggcattgc gctggccacc gaactggaaa ccgccggaca aaaatggtct
     2041 ctgggcttca cacccaaatt ccagcgtgtt gacctgttta actacaacgt gctggttaaa
     2101 aattacgaca gcagcgattt caaaggcgac cgttaccaca acaccagaaa cggtattaac
     2161 gccgatatcg gggccagtat ggacctggat gacaactgga cgctgggcct ggtcgcacaa
     2221 aacctgatcc cccgcagtat tgagacaaag gaagtgaacg gtatcactga aactttcagg
     2281 atccgcccac aggtgactgc cggtgtctca tggcacaacg acatgttcac caccgcactg
     2341 gatgttgacc tgacaccggc cagcggtttc acctccgaca gcaaacgtca gtttgcggcc
     2401 attggtgcag aatttaatgc ccggaaatgg gtgcagttgc gcgccggtta ccgtcagaat
     2461 ctggccagta acgacggcag cgccttcacc gccggtgtgg gcgtctcacc ctttgatgtg
     2521 gttcacctcg atctggccgg actggccgga actgacaata actacggtgc cattgctcag
     2581 ttacagttca ccttctgatt atcaggcccc gggactcccg gggcttctga cctgaatccg
     2641 cgcgtttccg ttctcctggc cactcactgc cagttctgca cattattttt ccggataaaa
     2701 ccatgccaaa aaaacatatc acccttgcag gggggatagt cgtgctcagt ctgagcggct
     2761 gccatcttcc cccggacaat accgcaccgg tcatgaagta cggtgcagct tctgaggttg
     2821 ccgtcacacg acatgctcag tatcagttat gggaacaggg cacgtattct gaagccagcc
     2881 cttcccggca ggccggtggc agcctgcagg cgcacagcgg gcgggtgtcc tttgactggg
     2941 aaggcgatgc cctggagtta ctcagcgaac tggcccgtct gcgcggacag cagttcagct
     3001 acagcggcgt ccgtcttccc ttacccgtct cactccatgt acgcgatatg accttcacag
     3061 atgtccttcg tctcacggag gcccagactg cctggcgggc agcactcaca cagtcagccg
     3121 gtcgtttaca tctttcttat atgccaacgg agaaaaaatg aaaaaacagg tctgtctcct
     3181 gctgtccggg ctcctgctgg caggctgtac cggacagcat caccattctt ctgccgtcgt
     3241 gacagaaaac gcaccgcccc cggatatccg tgcatggctg acgccctccg gaaaccagga
     3301 tgatggtatt tcagataccc gtcgtcagat gctcacggat gccggcagaa cgctgggatt
     3361 ccgtggtgga aaagcacagc gctcatggga actgattcag gcactgaatg cccgggaaag
     3421 taccctgaat gccctgtatg atttccggcc tctcatcagc aaggaaggct ggctgcctcc
     3481 tgttatcgat gaagctcagg atgttgcaca catcagaccg gaccagataa gaacagcctc
     3541 ccgtgtctgg accatcctcc gcccggaacg ctttgtcagt aatccacctg gatggcgtga
     3601 ctggctgctg aaaggacttt caaccaccgc cacaccaggc actgaaggcc gggttgtgcc
     3661 ggaagaccgg gcacaacgca gactatggga aaacgcctta cgtcagggat ggcaggaagg
     3721 gcgcctcaac gcagatctca cactggaagc taa
//
LOCUS       DAZFLD010000306         3740 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.308, whole genome shotgun sequence.
ACCESSION   DAZFLD010000306 DAZFLD010000000
VERSION     DAZFLD010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3740
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.308"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            132..1370
                     /gene="alaC"
                     /locus_tag="ACHKBL_004609"
     CDS             132..1370
                     /gene="alaC"
                     /locus_tag="ACHKBL_004609"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HFO5821357.1"
                     /translation="MADTRPERRFTRIDRLPPYVFNITAELKMAARRRGEDIIDFSMG
                     NPDGATPPHIVEKLCTVAQRPDTHGYSTSRGIPRLRRAISRWYQDRYGIEIDPESEAI
                     VTIGSKEGLAHLMLATLDHGDTVLVPNPSYPIHIYGAVIAGAQVRSVPLVEGVDFFNE
                     LERAIRESYPKPKMMILGFPSNPTAQCVELEFFEKVVALAKRYDVLVVHDLAYADIVY
                     DGWKAPSIMQVPGARDVAVEFFTLSKSYNMAGWRIGFMVGNKTLVSALARIKSYHDYG
                     TFTPLQVAAIAALEGDQQCVRDIAEQYKRRRDVLVKGLHEAGWMVEMPKASMYVWAKI
                     PEPYAAMGSLEFAKKLLNEAKVCVSPGIGFGDYGDTHVRFALIENRDRIRQAIRGIKA
                     MFRADGLLPASSKHIHENAE"
     gene            complement(1435..1506)
                     /gene="ypdK"
                     /locus_tag="ACHKBL_004610"
     CDS             complement(1435..1506)
                     /gene="ypdK"
                     /locus_tag="ACHKBL_004610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_077777854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YpdK"
                     /protein_id="HFO5821358.1"
                     /translation="MKYFFMGISFMVIVWAGTFALMI"
     gene            complement(1862..2782)
                     /gene="lpxP"
                     /locus_tag="ACHKBL_004611"
     CDS             complement(1862..2782)
                     /gene="lpxP"
                     /locus_tag="ACHKBL_004611"
                     /EC_number="2.3.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008951 - palmitoleoyl
                     [acyl-carrier-protein]-dependent acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009409 - response to cold [Evidence IEA];
                     GO_process: GO:0036104 - Kdo2-lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) palmitoleoyltransferase"
                     /protein_id="HFO5821359.1"
                     /translation="MFPQCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRI
                     GAMARPFLKRRESIARKNLELCFPQHSAEEREKMIAENFRSLGMALVETGMAWFWPDS
                     RVRKWFDVEGLDNLKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
                     MEWVQTRGRMRSNKAMIGRNNLRGIVGALKKGEAVWFAPDQDYGRKGSSFAPFFAVEN
                     VATTNGTYVLSRLSGAAMLTVTMVRKADYSGYRLYITPEMEGYPADENQAAAYMNKII
                     EKEIMRAPEQYLWIHRRFKTRPVGESSLYI"
     gene            3135..3377
                     /gene="yfdY"
                     /locus_tag="ACHKBL_004612"
     CDS             3135..3377
                     /gene="yfdY"
                     /locus_tag="ACHKBL_004612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdY family protein"
                     /protein_id="HFO5821360.1"
                     /translation="MIYLWMFLALCIVCVSGYIGQVLNVVSAVSSFFGMVILAALIYY
                     FTMWLTGGNELVTGIFMFLAPACGLMIRFMVGYGRR"
     gene            complement(3454..3681)
                     /locus_tag="ACHKBL_004613"
     CDS             complement(3454..3681)
                     /locus_tag="ACHKBL_004613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020232163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5821361.1"
                     /translation="MACNVFAFSISGGRSETSYKETEKTSVMTTTHSTKLQPSQAILF
                     KLREDAPPLNLTEDITPPFSTKANYLIHPVR"
BASE COUNT          953 a          879 c          949 g          959 t
ORIGIN      
        1 atttaacgtc attaataaaa atgttctaaa acgctggaca gacagaaatt aatcaggcta
       61 ttttcaaaac agccaccatc gtggcagcgt cgctcggacg gtccgggcgc taacgttaat
      121 ctgaggaaat tatggctgac actcgccctg aacgtcgctt tacgcgcatt gatcgtctcc
      181 cgccctatgt ttttaacatt accgctgaac tgaaaatggc tgcgcgtcgg cgcggcgaag
      241 atattattga tttcagtatg ggtaacccgg acggtgcgac tccgccgcat atcgtcgaaa
      301 aattatgtac ggtggcccag cgcccggaca cgcatggtta ctccacgtca cgcggcattc
      361 cgcggttacg tcgcgccatt tcccgctggt atcaggatcg ctacggcatt gagatcgacc
      421 cggaatcaga agccatcgtc actattggtt cgaaagaggg actggcgcat ctgatgctgg
      481 cgacgctgga tcatggtgac actgtactgg tgcctaatcc aagctacccg attcatattt
      541 acggcgcggt gattgccggg gcgcaggtac gctcagtgcc gctggtggaa ggtgtcgatt
      601 tcttcaacga actggaacgc gccattcgcg aaagttatcc gaaaccgaag atgatgatcc
      661 tcggcttccc gtcgaatcca accgcgcaat gcgtggagct ggagttcttc gaaaaggttg
      721 tggcgctggc gaaacgctac gatgtgctgg tggtccatga cctggcctat gccgatatcg
      781 tctacgatgg ctggaaagcg ccgtcaatca tgcaggtgcc aggtgcacgc gatgtggcgg
      841 tcgagttctt tacgctgtcg aaaagctaca acatggcggg ctggcgtatc ggctttatgg
      901 ttggcaacaa aacgctggtc agcgcgctgg cacgtattaa aagctatcac gattacggca
      961 cctttacgcc gttgcaggtg gcagcgattg cggcgctgga gggcgatcaa cagtgcgtgc
     1021 gcgacattgc tgaacagtac aaacgccgcc gtgatgtact ggtaaaaggg ctgcatgaag
     1081 cgggctggat ggtcgaaatg ccgaaggctt cgatgtatgt ctgggcgaaa atcccggaac
     1141 cgtatgctgc catgggctcg ctggaatttg ccaagaagct gctgaacgaa gcgaaggtct
     1201 gtgtctcgcc ggggattggc tttggcgact acggcgacac gcatgttcgc tttgcactga
     1261 ttgaaaaccg cgaccgtatt cgtcaggcga ttcgtggaat taaagcgatg ttccgtgccg
     1321 acggtttact acccgccagc agcaaacata ttcacgaaaa cgcggaataa acaccatcgc
     1381 cagaaatgca aacaggagcc agatggctcc tgtttttcat tgcacagttt gcttttagat
     1441 catcagggca aaagtaccgg cccaaacgat gaccataaaa gaaatgccca taaagaaata
     1501 tttcacttta catctctccg cgtaattact gttacgcctg aatgacttca ttacacctga
     1561 tacagtgagt tgacaccggg caaaacgacc ttaaaagtcc tgtattttac cgtgggcatc
     1621 accccagaat tctgagctaa actgctccag acggcgctaa tgtacgccta aaactgaaac
     1681 gagacaagag gcattttctt atttactttt agtaacttac aggtgtctca tatcggcgac
     1741 agtttaattt ctgacggtaa taattagaga ttcagcgaag gaaaaaacct taagaaaaaa
     1801 gccataaaaa ccatgaggtt attatggctg atttgaggag ggaaagagta agagcagttt
     1861 gttaaatgta tagcgacgat tctcccaccg ggcgcgtttt aaagcgacgg tggatccaga
     1921 ggtactgctc cggtgcgcgc atgatctctt tttcgataat cttgttcata taggcagcgg
     1981 cttgattttc atctgccggg tagccttcca tctctggggt gatgtacaaa cgatatccgc
     2041 tgtaatctgc ttttcttacc atcgttacgg ttaacatggc tgcgccagag agacgtgaaa
     2101 ggacatacgt accattggtt gtggcgacat tttccaccgc aaagaacggc gcgaaggagc
     2161 tgcctttacg accataatcc tgatcgggag caaaccatac cgcttcacct ttcttcagtg
     2221 caccgacaat gccgcgcaga ttatttctgc cgatcatcgc tttgttagag cgcatgcgcc
     2281 cacgggtctg cacccattcc atcagctgat tattatgtgg acgataggta gccatcattg
     2341 gttggcacag tcccatcacg cggccgccca gttccagcga cataaaatgg acgccgacaa
     2401 ccattacgcc gcgattttgc atttgtgcgc gtttaaggtt atccaaccct tcaacatcaa
     2461 accatttacg tacgcgactg tcgggccaga accacgccat gccggtttct accagcgcca
     2521 tgccgagtga acgaaagttt tcggcaatca tcttctcgcg ttcttccgca gaatgctgtg
     2581 ggaaacaaag ttccaggttt ttacgggcga tagattcacg ccgtttcagg aatggtcgcg
     2641 ccattgcgcc aatacgcgtg ccgagaaagc agagaacagg ataaggaagc tgtacccaaa
     2701 gccagagtac accaagccca aaccatgtga gccagtagcg aggatgtaga aactcgcggg
     2761 aaaatttgca ttgtggaaac atagttacct tctgtagata cgaattgttg tatcgcagaa
     2821 gattccatgt cgacagcggt acactcaatt ggttgggcca tcatgtacga caatggttcc
     2881 tgtaaagagt gtaaagacta gtcaggacat aaccacgagg agcatgaatg cggtggctct
     2941 actttatcac tggctgactg ctttcagcta ctgattatta atcgtgcaaa gtaaaaaatt
     3001 ttccgcacgc aatagcgttg catttaacgt gctgttttca ttgatgctta gcaaaaattg
     3061 ccagaggtta aggattgtct tactattaaa gtgactgaag ttacaggttt tttagcgcgg
     3121 caggaggttg agctatgatt tatttatgga tgtttctcgc cctgtgtatt gtctgcgtaa
     3181 gcggatacat cggtcaggta ctgaacgtgg tctcggcggt ctcttctttc ttcggcatgg
     3241 tgatcctcgc tgcactcatt tactacttca ccatgtggtt aactggcggt aatgaactag
     3301 tgacggggat atttatgttt cttgccccgg cttgtggctt gatgattcgc tttatggtgg
     3361 ggtatggcag gcgctagtgg gtcaggatgg ttcatttggg caggtgttca catcagcatc
     3421 aggcatgatt gttcacctgc caggtgacag acgctatcgc acaggatgaa taagataatt
     3481 cgcctttgtc gaaaaagggg gcgtaatgtc ttctgtgaga tttaatggtg gcgcatcttc
     3541 tctcaactta aacaaaatcg cctgtgatgg ctgaagtttt gtagaatgag ttgtcgtcat
     3601 cactgacgtt ttttctgtct ctttatagct cgtctcactt ctaccaccag aaatggaaaa
     3661 tgcaaaaaca ttgcatgcca taatagagac caataaaaat aagctaaaaa tcattaagtt
     3721 tactttcata gaaagttatc
//
LOCUS       DAZFLD010000307         3728 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.309, whole genome shotgun sequence.
ACCESSION   DAZFLD010000307 DAZFLD010000000
VERSION     DAZFLD010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3728
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.309"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            1..513
                     /locus_tag="ACHKBL_004614"
                     /pseudo
     CDS             1..513
                     /locus_tag="ACHKBL_004614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415162.4"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="YbeF family transcriptional regulator"
     gene            754..1719
                     /gene="lipA"
                     /locus_tag="ACHKBL_004615"
     CDS             754..1719
                     /gene="lipA"
                     /locus_tag="ACHKBL_004615"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HFO5821362.1"
                     /translation="MSKPIVMERGVKYRDADKMALIPVKNVATEREALLRKPEWMKIK
                     LPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCD
                     VAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKS
                     PQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLL
                     ERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQ
                     RYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEVK"
     gene            complement(1820..2023)
                     /gene="tatE"
                     /locus_tag="ACHKBL_004616"
     CDS             complement(1820..2023)
                     /gene="tatE"
                     /locus_tag="ACHKBL_004616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatE"
                     /protein_id="HFO5821363.1"
                     /translation="MGEISITKLLVVAALVVLLFGTKKLRTLGGDLGAAIKGFKKAMN
                     DDDAAAKKGADVDLQAEKLSHKE"
     gene            complement(2152..2940)
                     /locus_tag="ACHKBL_004617"
     CDS             complement(2152..2940)
                     /locus_tag="ACHKBL_004617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deaminated glutathione amidase"
                     /protein_id="HFO5821364.1"
                     /translation="MLVAAGQFAVTSVWEKNAEICASLMAQAAENEVSLFVLPEALLA
                     RDDHDADLSVKSAQLLEGEFLDRLRRESKRNMMTTILTIHVPSTPGRAWNMLVALQAG
                     NIVARYAKLHLYDAFAIQESRHVDAGNEIAPLLEVEGMKVGLMTCYDLRFPELALAQA
                     LQGAEILVLPAAWVRGPLKEHHWSTLLAARVLDTTCYMVAAGECGNKNIGQSRIIDPF
                     GVTIAAASEMPALIMAEVTPERVRQVRAQLPVLNNRRFALPQLL"
     gene            3033..3416
                     /gene="crcB"
                     /locus_tag="ACHKBL_004618"
     CDS             3033..3416
                     /gene="crcB"
                     /locus_tag="ACHKBL_004618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HFO5821365.1"
                     /translation="MLQLLLAVFIGGGTGSVARWLLSMRFNPLHQAIPLGTLAANLIG
                     AFIIGMGFAWFSRMTNIDPVWKVLITTGFCGGLTTFSTFSAEVVFLLQEGRFGWALLN
                     VFVNLLGSFAMTALAFWLFSASTAH"
     gene            complement(3470..3679)
                     /gene="cspE"
                     /locus_tag="ACHKBL_004619"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(3470..3679)
                     /gene="cspE"
                     /locus_tag="ACHKBL_004619"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HFO5821366.1"
                     /translation="MSKIKGNVKWFNESKGFGFITPEDGSKDVFVHFSAIQTNGFKTL
                     AEGQRVEFEITNGAKGPSAANVIAL"
BASE COUNT          963 a          975 c          855 g          935 t
ORIGIN      
        1 taatcaaatt gaaccctgtt taagcagaaa atcgtcagaa ggtaaaccac aaatatttac
       61 aaccttacga aatattgatc ttacccttct gactattttg aagttgcata tgtccataaa
      121 ggaatcgtta atgcagcgaa agtgcttaat ctgcccccct cggcaatcta tcagcctatt
      181 cagaaactgc gcgttatatt ccctgaccta ttgtttattc gcaaaggcca gggtgtcact
      241 cctaccgcat ttgcgatgca tctatatgag tctatcactc agggacttga gtccattctt
      301 ggcgcgctgg atatcgaagg aagctatgat aagcaacgga cgataactat tgcgccctcc
      361 ccctcggtcg gggcccaggt ccttcctgtc atctatcggg cgattaaaac tcactatcca
      421 cagcttttac tgcgcaaccc gcccatcggc ggcgcggaaa accaactctg tcagtttcaa
      481 accgatctca tcaccgataa catgttttgc acaggaccat agatgttatc cgtaatgcat
      541 tttgaaaaag tattgttaca gccagatcac agcgcagaac gataaaatgg cacaatccaa
      601 caacaatttt acattttcgc gaccgctttg gctgctttca ggtccgtttc aatgatatac
      661 tgccggtcgt taattcaaaa atagttgata attacaacaa tctattgaat tgaaacgctt
      721 tccttcgtaa ttcgcaactg gaacacgcac gctatgagta aacccattgt gatggaacgc
      781 ggtgttaaat accgcgatgc cgataagatg gcccttatcc cggttaaaaa cgtggcaaca
      841 gagcgcgaag ccctgctgcg caagccggaa tggatgaaaa tcaagcttcc agcggactct
      901 acacgtatcc agggcatcaa agccgcaatg cgcaaaaatg gcctgcattc tgtctgcgag
      961 gaagcctcct gccctaacct ggcggaatgc ttcaaccacg gcacagcaac gtttatgatc
     1021 ctcggcgcta tttgtacccg ccgttgtccg ttctgtgacg ttgcccacgg tcgcccggta
     1081 gctccggatg ccaatgaacc agtgaaactg gcgcaaacca ttgccgatat ggcgctgcgt
     1141 tatgtggtta tcacctccgt tgaccgtgat gacctgcgcg atggcggtgc ccagcacttt
     1201 gcggattgca ttactgccat tcgggaaaaa agcccgcaaa tcaaaattga aactctggtg
     1261 ccggatttcc gcggtcgtat ggatcgtgct ctggatattc tgaccgccac accaccagat
     1321 gtgttcaacc ataacctgga aaacgtaccg cgtatttacc gtcaggtacg gcctggtgca
     1381 gattacaact ggtcgctgaa gctgctggaa cgctttaaag aagcgcatcc ggaaatcccg
     1441 accaagtctg gtctgatggt gggtctgggt gaaaccaatg aagaaattat tgaggtaatg
     1501 cgcgacctgc gccgtcatgg tgtgacgatg ctaacgctgg ggcaatattt gcagccaagc
     1561 cgccatcacc tgccggttca acgttacgtt agcccggatg agttcgacga aatgaaagcc
     1621 gaagcgctgg cgatgggctt tacccatgct gcatgcggtc cgtttgtccg ctcgtcttac
     1681 cacgccgatt tgcaggcgaa agggatggaa gttaagtaat cctgtaataa ttacttacat
     1741 ctgtctgata aaaaacccgc tttttgaagc gggttttttg tatcgaacaa gatattgagg
     1801 gagcgtcctg ctcgccacgt cactctttat gagagagctt ttcagcctga agatcaacgt
     1861 ctgcgccttt tttcgccgca gcatcgtcat cattcatcgc cttcttgaac cctttaatgg
     1921 ccgctccaag gtctccgccc agcgtacgta acttcttagt cccaaacagc agaacgacca
     1981 gcgccgcaac taccagcagt ttggtaatac taatctcacc catagatacc ttcttgacat
     2041 ataaccggca acagaacgcg ccttaaaaac cagagaatat taacggtcat tcgacgcgcg
     2101 cacacaatag caatctgtaa caaggtgaat caagcgccga gtaaaaaaac atcataataa
     2161 ttgcggcagc gcaaagcgac ggttgtttaa gacgggcagt tgcgcgcgta cctgacgcac
     2221 acgttcgggc gtcacttccg ccataatgag tgcaggcatt tctgacgctg ccgcaatggt
     2281 gacgccaaag ggatctataa tccggctttg cccgatattt ttgttcccgc actcccccgc
     2341 cgccaccata taacaggtgg tatccagcac acgagcggca agcaacgttg accagtgatg
     2401 ctctttgagc ggcccacgaa cccaggcggc aggaagtacc aggatttcag ctccctgtaa
     2461 tgcctgtgcc agcgccagct ctggaaagcg taagtcataa caggtcatca gaccgacctt
     2521 catcccttcc acctccagta acggagctat ttcattacca gcatcaacat ggcgtgattc
     2581 ctgaatggca aatgcatcat agagatgcag tttggcataa cgggcgacga tgtttcctgc
     2641 ctgtagcgcc accagcatat tccacgcgcg ccccggtgtt gaaggaacat gaatcgtcag
     2701 aattgtcgtc atcatgttac gtttactttc tcgccgtaaa cggtcgagga attcgccttc
     2761 cagcagctgt gctgatttaa ccgaaagatc cgcatcatga tcatcacgcg ccagtaatgc
     2821 ttccggcagg acaaacagcg aaacctcgtt ttccgccgcc tgcgccatca acgaggcaca
     2881 aatctcagcg ttcttttccc acacagatgt aacagcaaac tgtcctgcgg caaccagcat
     2941 tgccacctcc tgtcagagtc gtaaaattcg ccaacggcga ttattcggcg ttacacttat
     3001 cactcataca aatcaaatag caggattttg cagtgttaca acttctttta gcagttttta
     3061 ttggcggtgg tacgggaagc gtggcgagat ggctgttaag tatgcgattt aacccgctgc
     3121 atcaggcgat tccgttgggg acgctggctg caaacctgat tggggcattc atcataggaa
     3181 tgggattcgc ctggttcagc aggatgacga acattgatcc agtgtggaaa gtattaatca
     3241 ccaccggatt ttgtggcggt ctaacaacct tctcaacatt ttcggcagaa gtggtgtttt
     3301 tgttacaaga gggccgcttt ggctgggcgt tactgaacgt tttcgtcaac cttttagggt
     3361 cttttgccat gaccgcactg gctttctggc tgttttccgc ctcaaccgca cactaaagac
     3421 caaaaaaaac ccgctgatta agcgggtttt gaattcttgc tgacgtatct tacagagcga
     3481 ttacgtttgc agcagaaggg cctttggcac cgttagtgat ttcgaactct acgcgctgac
     3541 cttcagcaag agttttaaaa ccattagtct ggattgcaga gaagtgtacg aacacgtctt
     3601 tgctgccgtc ttccggagta atgaaaccga atcctttgga ctcattaaac cacttaacgt
     3661 tacctttaat cttagacatc aaaattacct ttacatgaaa aatagacaca aatgctgtgt
     3721 cggttacc
//
LOCUS       DAZFLD010000308         3713 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.310, whole genome shotgun sequence.
ACCESSION   DAZFLD010000308 DAZFLD010000000
VERSION     DAZFLD010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3713
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.310"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            624..773
                     /locus_tag="ACHKBL_004620"
     CDS             624..773
                     /locus_tag="ACHKBL_004620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5821367.1"
                     /translation="MKPEHLYRLTGRDVLRYRRKNFDLMTGLAVATALGLIITFILLV
                     ARTTV"
     gene            770..1672
                     /locus_tag="ACHKBL_004621"
     CDS             770..1672
                     /locus_tag="ACHKBL_004621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5821368.1"
                     /translation="MSLETSLELNNQLLAQHNALLERLIRTMASGIVMHPDTISRVQE
                     CRDTATEAENMSAAMTLDDLEFSDVIALAGFYPVATPITEDMLQRAVAYRDAEGDKRV
                     VQIDALDSALQGVKRARALLKPALLDLSRNILKFWDDLPTIGERRAFAEQLLDAPADG
                     RDEVKPKRASNKDGERTGPFYVKNVSGTAASELHTLRKLNEMLKKGHIEINRVEYLQL
                     QEEFARRDAANSSQNNDAKDDHTIDFAALRKQAEGLILQLAKGGYRAEAIAILEKQGA
                     RKLGEVTDENLAEVITLAEKALEG"
     gene            1675..2976
                     /locus_tag="ACHKBL_004622"
     CDS             1675..2976
                     /locus_tag="ACHKBL_004622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001386185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2800 domain-containing protein"
                     /protein_id="HFO5821369.1"
                     /translation="MPDVHAQLSPSSAHRWLRCAGSLALEATQPDKETTFAIEGTAAH
                     ALAEKVLRNRQSHPEHYAGCNVSMFLGSYPLRENPDDTSGPQVDDEMVEAVGRYVDTV
                     WTLAKNNELLVEQRVDFSHITGVEESFGTADGIIIAGSELQIHDLKYGKGVRIDAEQN
                     EQLQLYALGALEQFSMLYDFETVRLFIHQPRLNHVSEWSLTVQELQSFGERALEAATS
                     AILVLNIAECEGIETLPLENFIPGEKQCRFCKAKAICTAQKMQHLQTAASDFEDLTKP
                     VSEIITNASARVPLLTIEQLAEIYSQADFIESWLKAVRDRVHNELNAGHPVPGFKLVT
                     GKQGNRAWSDEEAARALLKDQFRYKTEEVFDLKLISPTKAEKLIKKASPRRWSKVEAL
                     ITRADGKPTVVPESDPRPALNINPVNDFDDVSDDTLAADLI"
     gene            2992..3540
                     /locus_tag="ACHKBL_004623"
     CDS             2992..3540
                     /locus_tag="ACHKBL_004623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000769009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2815 family protein"
                     /protein_id="HFO5821370.1"
                     /translation="MKIKLNNVRLAFPALFEAKTVNGEGDPRFSAVFLMSPKHPQLEE
                     IRKAMKQVAKEKWGEKWESIYNQLEKKLNLCLHDGDEKAEYEGFPGNFFLNAANKARP
                     AVLDRDRSPLIQADGRPYAGCYVNAVIDIWAQDNNFGKRINASLGGVQFLRDGDAFAG
                     GGVASADDFDDISEGADAEALI"
     gene            3592..>3713
                     /locus_tag="ACHKBL_004624"
     CDS             3592..>3713
                     /locus_tag="ACHKBL_004624"
                     /inference="COORDINATES: protein motif:HMM:NF037896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nmad5 family putative nucleotide modification
                     protein"
                     /protein_id="HFO5821371.1"
                     /translation="MSQPVLTNHLKTQIINNALEKAGIPKRKSALRAARVEWAER"
BASE COUNT         1073 a          912 c          902 g          826 t
ORIGIN      
        1 tgacgcactt acaggtttgc ttgtattttt aaccgcaata agtaatcgct ctttaacaaa
       61 ctgaaccgcg tgacaggtaa gccgcagtac tcctggcaaa atgaaatggc acccgatggg
      121 atcgaggtaa gcgccgagtc cgtatgcgta cggtaagcgt agaggacaac accgcgacga
      181 gctgataagt cacgcaaatt gaaacgcccc gatgatgggg cgtctgaacc aatggaggaa
      241 aacatcatgc acaagtaaaa cgctcagacc gcagcagccg aaccaccaaa gagaactgaa
      301 tgaggaaacg gtgtgaaaac atataaaccg ctcaaaggtg aatgccctgc ttgccaagca
      361 caacttcagg caccaaagcc tgaacgatac caaatcaacg aatgttatga gcactgccct
      421 gaatgtggtg ctttcctgta cgccattgca acgcactggc gtgtccgctt caaccttgtg
      481 gtacctcgta cgcacaatac caactgaatc atccatgtaa ttgctgtgtg tagtctttgc
      541 ccgcctcacg tgacgggctt ttttatgtct gaaagcgcac tcgcaacagc gtgctccccg
      601 atatgaaaaa aggaatacaa ccaatgaaac ctgaacacct ctatcgactg acggggcgcg
      661 atgttctccg ttatcgccgt aaaaacttcg atttgatgac cggtctggcc gttgccactg
      721 cgctcggtct gatcatcact ttcattctcc ttgtagcgag gaccacagta tgagtttaga
      781 aaccagtctc gaacttaata atcaacttct ggcacaacat aatgcgctgc ttgaacgtct
      841 tatccgcaca atggcatcag gtattgttat gcatcctgac acgatttcac gagtgcagga
      901 atgtcgggat acagcaactg aagctgaaaa catgtcagcg gcaatgacac tggatgatct
      961 ggagttcagc gacgttatcg cactggctgg tttctacccg gtagccaccc ctatcacaga
     1021 agacatgctg caacgtgctg ttgcctaccg tgatgctgaa ggcgataaac gagtagttca
     1081 gattgatgct ctcgacagcg cattacaggg cgtcaaacga gccagggcgc tgcttaaacc
     1141 tgctctcctg gacctgtccc gtaacattct taagttctgg gacgacctgc caaccatcgg
     1201 cgagcgacgt gcttttgccg agcagctact tgatgcacct gcagatgggc gtgatgaagt
     1261 taagccgaaa agggccagta acaaagatgg agaacgcacg gggccgtttt acgtcaaaaa
     1321 tgtatccggc acagcagcca gtgaactcca caccttacgc aagttgaacg agatgctgaa
     1381 aaaaggccat atcgagatca accgtgttga gtaccttcag ctgcaggaag aatttgcacg
     1441 cagagacgca gcaaattcca gccagaataa tgacgccaaa gatgaccata caattgattt
     1501 cgcggcacta cgcaaacaag ctgaagggtt gatcctccag ttagcaaaag ggggttaccg
     1561 ggcagaagct attgcaattc tggaaaaaca gggagccagg aaacttggtg aagtaacgga
     1621 tgaaaatctc gctgaagtaa tcaccctggc tgaaaaagca ctggagggtt aatcatgcca
     1681 gacgttcatg cacaactttc tccatcatct gcacatcgtt ggctaaggtg tgcgggtagt
     1741 ctggcgctgg aggccacaca accggataaa gaaacaactt ttgcaatcga gggcactgca
     1801 gcgcacgcgc ttgctgaaaa agttctacga aacaggcaaa gccacccgga acactacgcc
     1861 ggatgcaatg tttctatgtt tctcggctca tacccccttc gcgaaaatcc tgatgataca
     1921 tctggcccac aggtggatga tgaaatggtc gaagccgtcg gccggtatgt tgatacggtc
     1981 tggactcttg caaagaataa tgaactattg gttgaacaac gtgttgattt ctcacatata
     2041 acgggtgtgg aagaatcttt cggaactgcc gacggcataa tcattgctgg tagcgaatta
     2101 caaatccacg acctgaaata tggcaaaggc gtccgcattg atgcagaaca aaatgagcaa
     2161 ctacaactgt atgctctggg tgctctcgaa caattcagca tgctgtatga ctttgagact
     2221 gtaagattat ttattcacca gccaaggctc aaccacgttt cagaatggtc tttaaccgta
     2281 caggaacttc agtctttcgg tgaacgggca ctggaggccg caaccagtgc gatccttgtt
     2341 ctcaatattg ctgaatgcga aggcattgag acactaccgc tggaaaactt catacctgga
     2401 gaaaaacagt gccgcttctg taaagcaaaa gctatttgca ctgcccagaa aatgcagcat
     2461 ttacaaacag cggccagcga ttttgaagat ctgacaaagc ctgtcagcga aataatcacc
     2521 aatgccagcg cacgtgtacc tctgttaacc attgagcagc ttgcggagat ctatagtcag
     2581 gccgacttta ttgaatcctg gctaaaggca gtacgggacc gggttcacaa tgaactcaat
     2641 gccggacatc cggtaccggg gtttaaactg gtaacaggaa aacaaggtaa ccgggcctgg
     2701 agtgatgaag aagccgcccg cgcgctgctg aaagaccagt tccgttataa aaccgaggag
     2761 gttttcgatc ttaagctgat tagcccaacc aaagccgaaa aacttatcaa aaaggccagt
     2821 ccgcgccgtt ggtcaaaagt cgaggcactg ataacacgag ctgatggtaa accaaccgtc
     2881 gttcccgagt cggacccacg ccccgcactc aatatcaacc ctgtaaatga tttcgacgac
     2941 gtatccgacg atacgctcgc cgcagacctc atctgattaa aggagacttt catgaagatt
     3001 aaattaaata acgtgagatt agcttttcct gctctgtttg aagctaaaac tgtaaacggc
     3061 gaaggcgatc cgcgtttctc cgcagtattt ttaatgtctc ccaaacaccc acaactggaa
     3121 gaaatccgta aagctatgaa gcaggtagcg aaggaaaaat ggggggagaa gtgggagtcc
     3181 atttataacc agctggagaa aaaactcaat ctgtgcctgc atgatggtga tgaaaaagca
     3241 gagtatgaag gcttccccgg caatttcttc ctgaacgctg ctaacaaagc gcgcccagct
     3301 gttcttgatc gcgatcgttc gccactaatt caggctgatg gacgtcccta tgcagggtgc
     3361 tatgtaaacg ccgttatcga tatctgggca caggacaata atttcggtaa acgcattaat
     3421 gcctcactcg gcggagtcca gttcctgcga gacggcgatg cattcgctgg cggcggagtg
     3481 gcaagcgctg acgatttcga cgatatcagc gaaggtgctg atgctgaagc actaatttaa
     3541 aaatattcat cccccagcta cgtgctgggg tatctcatga gggaaagaat aatgtcacag
     3601 cccgtattaa ctaatcacct taaaactcaa atcatcaata acgcacttga gaaagcgggt
     3661 atccccaaac gtaaatcagc actacgagct gctcgtgttg aatgggccga acg
//
LOCUS       DAZFLD010000309         3689 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.311, whole genome shotgun sequence.
ACCESSION   DAZFLD010000309 DAZFLD010000000
VERSION     DAZFLD010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3689
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.311"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..789)
                     /locus_tag="ACHKBL_004625"
     CDS             complement(<1..789)
                     /locus_tag="ACHKBL_004625"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000712975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HFO5821372.1"
                     /translation="MKKFDVAALGSGNIDIFLSIPSLPTRGGKVVGTRLGEQAGGTVA
                     NSACAMGQLGLNVVSVSCVGNDHSASIILDGFKKYHVNCDFVQVIPELIANTAIIFID
                     ELGEKTLVYSPGSDHEWDKEKALQAIAQSRYFYTMPADIEKFRMLAEYSRSQMTKVVV
                     DIEPHIIATPEHLARILHLADIAIFNYDGFIRGYATEPDFTLLHDIQDEYQLDAVVVT
                     LDVRGVIAVKGNEQVKIGSYTIPVIDTTGAGDTFNGAFVYSLIKN"
     gene            complement(794..1792)
                     /locus_tag="ACHKBL_004626"
     CDS             complement(794..1792)
                     /locus_tag="ACHKBL_004626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFO5821373.1"
                     /translation="MNMPARTPVMPLAQTREEINATAASLRHLAAIWGECFSTFQLDK
                     RAVERVCIVGSGDSWTVALCAAAWLGKYTHLFCYALQTWDFLQTDLTRYQKETLIIIL
                     SASGRPSMTVDALCHAVCSNAQVLGVTNCPGTPFCAITENMLYTWANKQGIPTQSSSV
                     TLYALLRLAQKLCPGLTPLQIEDDLEGKFSQINQHWQQKKRHFYQQKEITFLGSGLSW
                     GLAISGSNLLSCGPQIRATALPLEEFYHSLRLHQAGVGQHYILLPATSCESPFYLATQ
                     KAIVEQGATAELISFIPDASESNNLFLVMQWLYEMCWHLSCDYVDAGGQRVSHREK"
     gene            complement(1792..2868)
                     /locus_tag="ACHKBL_004627"
     CDS             complement(1792..2868)
                     /locus_tag="ACHKBL_004627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HFO5821374.1"
                     /translation="MNKIEQAVIYNKILGSLACAGMGDALGAATELYSIDEIKAQWGG
                     FLNAFVSPPADTFAGSLNGIAGLITDDSSQMYVFSEGLIEAGFDNFTNNDWLACLLRW
                     ADMQPYANYKGPTTEQIVKALKEGRPTNTIGRIGTSSRQAPNVGTTNGAGMRVAPAGL
                     IWSGKKEKACHLALITCLPSHDTNIAIASACAIAAATSQAMLPEASLTSLLDAAIWGA
                     NYGERLAKQYARCVAGPSIAMRIQLAADIARRANDLESCLREMEGLVGNSVAAHESIP
                     AAIGLLLYCKGEPWETIHACANIGNDTDSIATMAGAIAGAWRGFDALPEDKYAFFRAV
                     NNKDFDIEAIASGLTLLALEAQEK"
     gene            complement(2882..3634)
                     /locus_tag="ACHKBL_004628"
     CDS             complement(2882..3634)
                     /locus_tag="ACHKBL_004628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000591154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFO5821375.1"
                     /translation="MIKIYKASKGQLKHREIFDEIKKKVDNGEWKEGKAIPTERELSE
                     YYLSSRTTIRKAIESLKQRGYLHSVHGQGTFVLPSRSRENHLLHSFTDDIKARGGIPA
                     QNILEIGYVPVSDVIRKNLDLDIHVQTVFCIKRVRYMGSTPLGIQTSWLALNDRQEIT
                     QEDLLATGSLYILLEEKLAIKPLEATEIISARLPSPLERQLLELTADDVVLSCTRVTL
                     SVERKPMEYVEMVYPASRYSYKARITKDSFSV"
BASE COUNT          973 a          863 c          784 g         1069 t
ORIGIN      
        1 attttttatt aagctataaa caaatgcacc attaaaagta tctccagcgc ctgtggtatc
       61 aataacggga attgtataac tacctatttt tacttgctca ttgcctttga ctgcaattac
      121 tcccctaaca tccagcgtaa ccacgacagc gtcaagttga tactcgtctt gtatatcatg
      181 cagaagtgta aaatctggct ctgttgcata gccacgaata aaaccgtcat aattaaatat
      241 cgcaatatca gcaaggtgta aaattctggc aaggtgttca ggtgttgcaa tgatatgagg
      301 ctcaatatca acgacaactt ttgtcatctg gctacgggaa tattcggcca gcatacgaaa
      361 tttctcgata tctgctggca tcgtataaaa ataacggctt tgcgcaattg cctgcagggc
      421 tttttcttta tcccactcat gatcactacc aggcgagtaa accaacgttt tttcacctaa
      481 ttcatcaata aagataatcg cggtattggc aatcaattcc ggaattacct gaacgaaatc
      541 acaatttaca tgatattttt taaatccatc aagaatgatc gaggctgaat gatcattgcc
      601 cacacatgaa acagatacca cattgagacc cagttgtccc atcgcacagg cactgttggc
      661 gactgtgccg ccagcttgtt cacccagacg tgtcccgaca actttaccgc cacgagtggg
      721 gagtgacggg atagataaaa aaatatctat atttccactg cctaaagcgg cgacatcaaa
      781 tttcttcata gtcttatttc tccctatggc taacgcgttg cccgcctgcg tcgacataat
      841 cacaacttaa atgccagcac atttcatata accactgcat gaccagaaat aaattgttgg
      901 actcactggc gtcaggtata aaactgatta attctgctgt cgctccctgt tcaacaattg
      961 ctttttgggt tgcaaggtaa aatggggact cgcaggatgt ggctggtagt aaaatataat
     1021 gctgaccgac accagcctga tgtaagcgta atgaatgata aaattcttcc agtggaaggg
     1081 ctgtcgcacg aatctgtggg ccgcaagaaa gtaaattgct ccccgatatt gccagtcccc
     1141 aacttaagcc actcccaaga aacgttatct ctttttgttg gtaaaaatgg cgcttctttt
     1201 gctgccagtg ctgattaatc tggctaaatt tcccttccaa atcatcttca atctgcagtg
     1261 gtgttaagcc tggacataat ttttgcgcta agcgcaggag ggcataaagt gtgacgctac
     1321 tactttgcgt tggaataccc tgcttattcg cccatgtata gagcatgttt tctgttattg
     1381 cgcaaaatgg tgttccaggg caattcgtca cacccagcac ttgcgcgttt gagcatactg
     1441 cgtggcagag ggcgtcaacg gtcattgatg gtctgccact tgcactgaga ataatgatta
     1501 acgtttcttt ctgataacgg gttaaatcag tctgaagaaa atcccacgtt tgcaatgcgt
     1561 aacaaaacag atgtgtgtat ttccccagcc aggcggcggc acatagtgcc accgtccagg
     1621 agtcgcccga tcccactata cagacgcgtt caacagcacg tttatcgagc tgaaaagtag
     1681 aaaaacattc accccagata gctgccaaat ggcgtaacga tgccgccgta gcatttatct
     1741 cctcacgagt ttgcgctaat ggcataacag gagtacgggc tggcatgttc attatttctc
     1801 ctgtgcctct aatgcaagca atgtcagacc cgatgcgatc gcttcaatat cgaaatcttt
     1861 attatttacg gcgcggaaaa aggcatattt atcttcaggg agcgcatcaa aaccgcgcca
     1921 tgcccctgca attgcgcctg ccatcgtagc gatgctatcg gtgtcattgc caatattggc
     1981 gcaggcatga atggtttccc agggttctcc tttgcagtac agcaatagac cgattgccgc
     2041 aggaatggat tcatgggcgg caacagagtt accaaccaaa ccttccattt ctcgcagaca
     2101 agactccagg tcattggctc gtctggcgat gtccgctgca agttggatgc gcatggcgat
     2161 agatggccct gcgacacatc gtgcatattg tttggccagg cgttcgccgt aattcgctcc
     2221 ccagattgcg gcgtccagta atgatgtgag cgaagcttct ggtaacatag cttgtgatgt
     2281 cgccgcagcg atggcgcaag cgctggcaat cgcaatattg gtgtcatgcg aaggtaggca
     2341 agttattagc gccaggtgac acgctttttc tttcttgcct gaccagatca atcctgcagg
     2401 ggctacacgc attcctgctc cattcgtggt gccgacattg ggtgcctgac gagaagatgt
     2461 tccaatacgc ccaatggtgt ttgttggtct accttctttt agtgccttga cgatttgttc
     2521 tgttgtgggg cctttataat ttgcgtaggg ctgcatatcg gcccaacgca gaagacaagc
     2581 taaccagtca ttattggtga agttatcaaa tccagcctca attaaccctt ctgaaaacac
     2641 atacatttgg ctggaatcgt cggtgataag tcccgcaata ccatttaatg agccagcgaa
     2701 tgtatcggca ggaggagaaa caaaggcatt taaaaaacct ccccattgag cttttatttc
     2761 atcaatgctg tagagttcag tcgctgcgcc taatgcatcg cccatacctg cgcaggctaa
     2821 agaacctaat attttgttat aaattacagc ttgttctatt ttgttcacaa tattctctct
     2881 gttacacgct aaaactgtct tttgtaattc gtgctttgta ggagtaccgg cttgcgggat
     2941 acaccatctc tacatactcc attggtttgc gttccacgga taacgtgacg cgggtacaac
     3001 ttaatacgac atcatcggca gtcagttcca gtaattgacg ctcaaggggc gagggaagcc
     3061 gggcagagat aatttctgtt gcttctaaag gtttaatagc gagtttttct tccagtaaaa
     3121 tatagagcga acccgtcgcc agcagatctt cttgggttat ttcttgcctg tcatttaatg
     3181 caagccatga tgtctgaata cccagggggg tacttcccat atagcgaacg cgttttatac
     3241 agaatacggt ttggacatgg atatccaggt cgagattttt tctgataaca tcactgacag
     3301 gaacatagcc tatctcaaga atattctgtg ctggtatacc acctcgagct ttaatatcgt
     3361 cggtaaaact atgcaataaa tgattttctc tactacgaga aggcaataca aatgttcctt
     3421 gcccgtggac actatgcagg tagccgcgtt gtttaagact ttctattgcc tttcgaatag
     3481 ttgttctgct acttaaataa tattctgaca attccctttc tgtcggtatg gctttgcctt
     3541 ctttccattc accgttatct acttttttct ttatttcatc aaagatttca cgatgcttta
     3601 gctgaccttt actggcttta taaattttta tcatgacaaa gcctcatttc aagtggttga
     3661 taccaactct acattcttta acttttatt
//
LOCUS       DAZFLD010000310         3672 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.312, whole genome shotgun sequence.
ACCESSION   DAZFLD010000310 DAZFLD010000000
VERSION     DAZFLD010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3672)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3672
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.312"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..118
                     /gene="ymgI"
                     /locus_tag="ACHKBL_004629"
     CDS             <1..118
                     /gene="ymgI"
                     /locus_tag="ACHKBL_004629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protein YmgI"
                     /protein_id="HFO5821376.1"
                     /translation="ITVTLILNYLNNSERSLVKQILIEDEIIVCATYLIPDI"
     gene            120..464
                     /gene="ymgG"
                     /locus_tag="ACHKBL_004630"
     CDS             120..464
                     /gene="ymgG"
                     /locus_tag="ACHKBL_004630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415690.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YMGG-like glycine zipper-containing protein"
                     /protein_id="HFO5821377.1"
                     /translation="MKKKILAFGLISALFCSTPAMADMNRTTKGALLGAGVGLLTGNG
                     VNGVLKGAAVGAGVGAVTEKGRDGKNARKGAKVGAAVGAVTGVLTGNGLEGAIKGAVI
                     GGTGGAILGKMK"
     gene            468..803
                     /gene="ymgD"
                     /locus_tag="ACHKBL_004631"
     CDS             468..803
                     /gene="ymgD"
                     /locus_tag="ACHKBL_004631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmgD family protein"
                     /protein_id="HFO5821378.1"
                     /translation="MMMKKFALLAGLFVFAPMTWAQDYNIKNGLPSETYITCAEANEM
                     AKTDSAQVAEIVAVMGNASVASRDLKIEQSPELSAKVVEKLNQVCAKDPQMLLITAID
                     DTMRAIGKK"
     gene            complement(861..3503)
                     /locus_tag="ACHKBL_004632"
                     /pseudo
     CDS             complement(861..3503)
                     /locus_tag="ACHKBL_004632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768537.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ECIAI39_1904 family autotransporter adhesin"
BASE COUNT          982 a          875 c          797 g         1018 t
ORIGIN      
        1 catcacagtc acgctcattt tgaattatct gaataatagc gagcgttctt tagttaagca
       61 gatcttaatt gaagacgaaa ttattgtgtg tgcaacttac ttaataccgg acatctaagt
      121 tgaagaaaaa aatacttgcc ttcgggctta tctcagcatt gttctgctcc acgccagcga
      181 tggcggatat gaaccgcacc acgaaaggtg cattgctagg cgcaggcgtt ggtttactta
      241 ctggcaatgg tgttaatggc gtactgaaag gtgcagctgt tggcgctggt gttggtgcag
      301 taacagaaaa aggccgcgac ggtaaaaatg cgcgtaaagg tgcaaaagtg ggtgccgccg
      361 ttggtgctgt cactggcgtt ctgaccggca atggcctcga aggggcgatt aaaggtgccg
      421 tgattggcgg tacgggtggt gcaattctgg gtaaaatgaa ataaggaatg atgatgaaaa
      481 aatttgcttt actggcgggt ctgtttgttt tcgcaccaat gacctgggcg caggactaca
      541 atattaaaaa cggtttgccg tcagaaacct atattacttg cgcagaagcc aacgaaatgg
      601 caaaaactga cagcgcacag gttgcagaaa ttgttgcggt aatgggtaat gccagcgttg
      661 ccagccgtga tttaaaaatt gagcaatctc cggaactaag cgccaaagtg gtagaaaaac
      721 tgaatcaggt ttgcgctaaa gacccgcaaa tgctgttgat cacggcaatt gatgacacca
      781 tgcgtgctat cggcaaaaaa taatatttca tatgttaccg cgtcgatgaa ttcgacgcgg
      841 ttttttagct aatggcggga tcagaactta tatttcaggc ccaccatcat tgcggtgtca
      901 ttgtagccat tatcccccag ctgcacgccc acattacccc agacgctggc cgccggattc
      961 agattgccat ttacgccaag tttaatttcc cccagattac gcgccccgtc ttgttcaact
     1021 ttcacaccgt tcattgagac agcaaaatct ttactgttat ggatccagtt catttcaaca
     1081 aaaggttcaa agtcgtcacc gtgcggaccg ctgtttttct cctgagtacg aataaaggtg
     1141 cggaagccga gtcgggtctg gatgttgtta tttgcgtcat tctcaatacg tgagccattg
     1201 gcttccgtgt gcgcattatg atcgacgccc atccagatag cctgtggctg gaagagccac
     1261 gtatatttcg ccgcttccag accaaaggat ttattcaggg caaaaccgta acccgcttcc
     1321 agcgagacgg ttgcaccttt cgcgctatag gattcatagg ctaattcatc ccctttcacc
     1381 gagtttttaa accagctata ttacgcccag gtgtcaatgt atgcgccttt tttactgatg
     1441 tcatcggcaa accaggtggc atacagccct gcgctatagc ctctgacact ccctttcgaa
     1501 cgataatccg acacgctgga atgagttgag ttgtagtcgc gggcataacc agccaacact
     1561 cccaaacgcc aactgtcact gtcggtaaaa ccaccggtta acagatccgc ccctaactgc
     1621 gagacgtagc gatgcgaggt cgttctcaac tgtccgttgc tgtcacgcca ggcattatgc
     1681 ccgccaattt gacgtagcca aagccggctt gaacgctcct gttaagttac aggatcgatg
     1741 taacgcgttt cacctgcacg atcgttcaga cgcataacaa aaagagtgtt agcggctgcc
     1801 aggttggcga cgtaacttcc agcttcaggg cgcaggacgg gggtaggcgc aacagtaggc
     1861 gctgaacttc ctccggagtt tgtttcaccg ccgctcgttg cctggaagtt agtcagatac
     1921 cagtttttat tggtcgcttc ggtattacct cgggttaatg aatattcgta ggcaccggcg
     1981 aaaatcctcg aatccttaat aaattctcca tttgattccc cctcaacgct gataatctct
     2041 atcccattta acgtgtaagc cccgctgccg ccagcattgg taactttaac tcgggttgtt
     2101 ccagaggtat ttccattaat aacgacttta tccgtagctg aattatcatc acccaattca
     2161 gttctgagat gcaatacgcc attattacca acgtaatttt cagttattgt caatcttgtt
     2221 ggttcgaaat cctttccatc tgcccgggaa atatagaccg tactattatc aaccgtgagt
     2281 ttgctcaccg ttgagtcagc tttaatatcc caggtactat tatctgaaag cgacagatag
     2341 gtatgagtgt tatcatctgt tgaaagattg gctgaacccg ttatttttga attagttgca
     2401 tcgacggtca aatgaccttt aatattatta ataaaaacat ctgcagccga tgatacctca
     2461 ctatccgtaa tggtcacgtt actcgtctca tttttatggt agaggatttc atttgctgac
     2521 cgtagtgagc taccagataa aatatctacc gttgcttcct gaccatttat ggaagactcc
     2581 agcacaattc cagccccctg atgcgcgact gcagatgtat ttttcagcgt caccttgccg
     2641 ccattataaa ttttagcaat cgcggcgtca ggacccgttg tataaatttc tccctggtta
     2701 aatgaaactg tcccctggcg agcagcgatg gcatgagcat tgtccccatg cgtggtgata
     2761 tcaacatcgg ttaatgtaac gctggcattg gtataatcaa gcttgctacc ctccttgacg
     2821 acagtgccat tagcgtaagc ggcataactt tctgttccgt gggttgttat ctcccctttt
     2881 gccatatcca gttttgcttt ggcgttattt tcattacttc ctgtatcggc agtactttct
     2941 tcatcaagag aagatacaca cgcacgcgcg cttccgggag atgcacacgc aagaacgccg
     3001 actgcgtgat tacccgcagt aacgataaaa gtattacctc cgtcagaaaa agagggggta
     3061 gccgaaccgt cagggttgac tcttattatg gtatcagtat tcaggtttcc atcaacttga
     3121 cttaacaaac caacagaata atctcccgtc gtgacaatcg tattattacc ccgcaaagtg
     3181 acagcaccgc catattcact tacgactgcg ctagctgtat atgctgtgct tagagaacta
     3241 ttgtcagaag gatataaacc gcccgttgtt acaatgacgc cattgttaat ggttaaattg
     3301 ctaccctttc ctgcaacaga tacaccgtaa cttccattac ccgttgttat aactagcgca
     3361 ttatcaatat cttttacttc tccatctttt atttcaacaa catgtttagg ttcatcgtgc
     3421 tgaataaact gttcagcata tgcattaccc accgccaaag caataagtcc taaactaaac
     3481 ccggggagtt ttttcagttt catagattaa ccatccattc attggattaa caaacattcc
     3541 tggtcaaata acctatgagt tcagattacc aaaacggagg gtaaagacaa aaacaaaagc
     3601 acaaaaaatt taaatagata aacagcgaca atatataaat gataaataag gcggcattat
     3661 catttttcac ct
//
LOCUS       DAZFLD010000311         3671 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.313, whole genome shotgun sequence.
ACCESSION   DAZFLD010000311 DAZFLD010000000
VERSION     DAZFLD010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3671
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.313"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(49..612)
                     /gene="pabA"
                     /locus_tag="ACHKBL_004633"
     CDS             complement(49..612)
                     /gene="pabA"
                     /locus_tag="ACHKBL_004633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 2"
                     /protein_id="HFO5821379.1"
                     /translation="MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKP
                     QKIVISPGPCTPDEAGISLDVIRHYAGRLPILGVCLGHQAMAQAFGGKVVRAAKVMHG
                     KTSPITHNGVGVFKGLANPLTVTRYHSLVVEPDSLPECFEVTAWSETREIMGIRHRQW
                     DLEGVQFHPESILSEQGHQLLANFLHR"
     gene            complement(644..1246)
                     /locus_tag="ACHKBL_004634"
     CDS             complement(644..1246)
                     /locus_tag="ACHKBL_004634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adenosine monophosphate-protein
                     transferase Fic"
                     /protein_id="HFO5821380.1"
                     /translation="MSDKFGEGRDPYLYPGLDIMRNRLNIRQQQRLEQAAYEMTALRA
                     ATIELGPLVRGLPHLRTLHRQLYQDIFDWAGQLREVDIYQGDTPFCHFAYIEKEGNAL
                     MQDLEEEGYLVGLEKAKFVERLAHYYCEINVLHPFRVGSGLAQRIFFEQLAIHAGYQL
                     SWQGIEKEAWNQANQNGAMGDLTALQMIFSKVVSEAGESE"
     gene            complement(1236..1403)
                     /locus_tag="ACHKBL_004635"
     CDS             complement(1236..1403)
                     /locus_tag="ACHKBL_004635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfG family protein"
                     /protein_id="HFO5821381.1"
                     /translation="MKKLTDKQKSRLWELQRNRNFQASRRLEGVEMPLVTLTAAEALA
                     RLEALRRHYER"
     gene            complement(1508..2080)
                     /gene="ppiA"
                     /locus_tag="ACHKBL_004636"
                     /gene_synonym="rotA"
     CDS             complement(1508..2080)
                     /gene="ppiA"
                     /locus_tag="ACHKBL_004636"
                     /gene_synonym="rotA"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase A"
                     /protein_id="HFO5821382.1"
                     /translation="MFKSTLAAMAAVFALSALSPAAMAAKGDPHVLLTTSAGNIELEL
                     DKQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPPIKNEAD
                     NGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVKGMDVAD
                     KISQVPTHDVGPYQNVPSKPVVILSAKVLP"
     gene            2351..3532
                     /gene="tsgA"
                     /locus_tag="ACHKBL_004637"
     CDS             2351..3532
                     /gene="tsgA"
                     /locus_tag="ACHKBL_004637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter TsgA"
                     /protein_id="HFO5821383.1"
                     /translation="MTNSNRIKLTWISFLSYALTGALVIVTGMVMGNIADYFNLPVSS
                     MSNTFTFLNAGILISIFLNAWLMEIVPLKTQLRFGFLLMVLAVAGLMFSHSLALFSAA
                     MFILGVVSGITMSIGTFLVTQMYEGRQRGSRLLFTDSFFSMAGMIFPMIAAFLLARSI
                     EWYWVYACIGLVYVAIFILTFGCEFPALGKHAPKTDAPVEKEKWGIGVLFLSVAALCY
                     ILGQLGFISWVPEYAKGLGMSLNDAGTLVSNFWMSYMVGMWAFSFILRFFDLQRILIV
                     LAGLAAILMYVFNTGTPAYMAWSILALGFFSSAIYTTIITLGSQQTKVPSPKLVNFVL
                     TCGTIGTMLTFVVTGPIVEHSGPQAALLTANGLYAVVFVMCFLLGFVSRHRQHNTLTS
                     H"
BASE COUNT          866 a          966 c          886 g          953 t
ORIGIN      
        1 ttataatcac aaaatatgca taaaaaatca ctgaatggca atcagaaatc agcgatgcag
       61 aaaattagcc agcagttgat gtccttgttc gctgagaata ctttctggat ggaattgcac
      121 accttccaga tcccactggc gatggcgaat ccccataatc tcgcgggttt cgctccaggc
      181 cgtcacttca aagcactccg gtaatgagtc aggttccacc accagcgaat ggtagcgtgt
      241 cacggtgagt ggatttgcca gccccttaaa tacgcccaca ccattatgtg taatcggcga
      301 ggttttgccg tgcatgacct ttgcggcgcg cacaactttg ccgccaaatg cctgcgccat
      361 tgcctgatga ccgaggcaga cgccaagaat cggcaagcgt ccggcatagt gacgaataac
      421 gtcaagggag atcccggctt catctggcgt acaggggccg ggtgagatga caattttttg
      481 tggtttaagg gcgtcgatat ccgccaacgt caacgcatcg ttgcgcttaa ccaacacatc
      541 cgcccccagt tcacaaaagt actggtagag gttccaggta aaagaatcgt agttatcaat
      601 aagcaggatc atggcggctc cggtacgaaa gaaccgcgct attttactca gattccccgg
      661 cttcgcttac cactttgcta aatatcatct gcagcgcggt gagatccccc attgccccat
      721 tctgatttgc ctgattccag gcctcttttt caataccctg ccagctcagt tgatatccgg
      781 catgaatcgc cagttgctcg aagaagatcc gctgtgccaa accacttccc acccggaagg
      841 gatgcagcac gttgatttca caatagtaat gcgccagccg ctcgacgaac ttcgctttct
      901 ccaggccaac cagataacct tcttcctcca gatcctgcat cagggcattg ccctcttttt
      961 cgatataagc aaagtggcag aacggcgtat cgccctgata aatatcgact tcgcgcagtt
     1021 gccccgccca gtcgaaaata tcctgataca actggcgatg aagagtacgc aaatgcggta
     1081 aaccccgaac caacgggcca agttcaatgg tcgcagcacg cagcgccgtc atttcgtaag
     1141 cggcctgttc cagccgctgc tgctggcgga tgttcagccg gttacgcatg atatcaaggc
     1201 ctggataaag atacggatcg cgcccttcgc cgaatttatc gctcatagtg cctcctcagc
     1261 gcttcaagac gcgctaaagc ctctgcggca gtaagagtga ctaaaggcat ctcgacgcct
     1321 tcaagacggc gactggcctg gaaattacga ttacgctgaa gctcccagag acgggacttt
     1381 tgcttatcgg tgagtttctt cacttgatgc ctccctgaac gtgtccattt gccacaagta
     1441 taagcggcaa atccgggtta cgccgggaga gcagaagcgc gggcaagatt gcccgcgcga
     1501 gaaatcatta cggcaggact ttagcggaaa ggataactac cggttttgac ggcacattct
     1561 ggtacggacc aacgtcatga gtcggcacct gggaaatctt atcggcaacg tccatgcctt
     1621 tcaccacttt accaaatacc gcgtaaccga aatcacgctg accgtggtca aggaaggcgt
     1681 tatcggcaac gttgataaag aactggctgg tggcgctgtc tttgtcagcg gtacgcgcca
     1741 tcgcgatggt gccacgcgtg ttgcgcaggc cgttatcagc ttcatttttg attggcgggt
     1801 ttggtttttt ctgctgcatc tgctcggtga aaccgccgcc ctgaatcata aagccaggaa
     1861 tgacgcggtg aaaggtcgtg ttgttataaa aaccactgtt cacataatcg acaaagtttt
     1921 gcacagacac tggcgctttt tgtttatcca gctccagttc gatgttacca gctgaggttg
     1981 tcaacaatac gtgcgggtcc cctttcgctg ccattgctgc gggagaaaga gcagaaagag
     2041 cgaaaacagc agccatcgcc gccagggtcg atttgaacat gagatttcct taacaaacag
     2101 agcagaaaaa aagcaagtga agagattcta aatagccttc aagaacaagg ccatcccttt
     2161 acctaatttt acgtatctga aatatatgta acctgaggat aactttttat tccgtgatca
     2221 aaatcacctc ttaaaatgca atttagcaac cgattgcaat aaaacattta atcagatcac
     2281 aaaatcgcct aaaatcgccc gctcgcggca tagcccgcta tgccatttta tttttgaacg
     2341 caggccaatc atgactaaca gcaatcgcat caagctcaca tggattagct ttctttccta
     2401 cgcactgacc ggtgcgttgg ttattgtcac cgggatggtg atgggaaata tcgccgatta
     2461 tttcaatctg cctgtttcca gcatgagtaa caccttcacc ttcctcaatg ccggcatttt
     2521 aatctctatc ttcctcaacg cctggctgat ggaaatcgtc ccgttgaaaa cgcagttacg
     2581 ttttggcttt ctcctgatgg tgctggcggt tgccggtttg atgttcagcc acagcctggc
     2641 gctgttctcg gcggcgatgt tcattctcgg ggtggtcagc ggcatcacca tgtcgattgg
     2701 tacattcctg gtaacacaaa tgtatgaagg acgtcagcgc ggttcacgcc tgttatttac
     2761 cgactccttc ttcagtatgg ccgggatgat tttcccaatg atcgccgcgt ttctgctggc
     2821 acgtagcatt gagtggtact gggtttatgc ctgcatcggg ctggtgtatg ttgctatttt
     2881 tattctgacc ttcggctgtg aattcccggc actgggcaaa catgcgccaa aaacagatgc
     2941 tccggtagag aaagaaaagt gggggatcgg cgtactgttt ctctctgttg cggcgctgtg
     3001 ctacatcctc ggtcagttag gttttatctc ctgggtgccg gaatatgcca aaggcctggg
     3061 catgagcctg aacgacgcag gcacgctggt aagtaacttc tggatgtcgt acatggtcgg
     3121 catgtgggcg ttcagcttta ttcttcgctt ctttgatttg caacgtatcc tgatcgtact
     3181 ggctggtctg gctgcgattc tgatgtacgt ctttaacacc ggaacaccgg catatatggc
     3241 gtggtcaatt ctcgccctgg gcttcttctc cagcgcgatc tataccacca tcatcaccct
     3301 gggttcacag cagaccaaag taccgtcgcc aaaactggtt aactttgtcc tgacctgcgg
     3361 gaccatcggt actatgttga cctttgtggt taccggcccg attgttgaac atagtggtcc
     3421 gcaggcggcg ctgctgacgg caaacggtct gtacgctgtc gtctttgtga tgtgcttcct
     3481 gttaggtttc gtcagccgtc accgtcagca taacaccctg acctctcatt aattgttcat
     3541 gccgggcggc actatcgtcg tccggccttt tcctctctta ctctgctacg tacatctatt
     3601 tctataaatc cgttcaattt gtctgttttt tgcacaaaca tgaaatgtca gacaattccg
     3661 tgacttaaga a
//
LOCUS       DAZFLD010000312         3646 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.314, whole genome shotgun sequence.
ACCESSION   DAZFLD010000312 DAZFLD010000000
VERSION     DAZFLD010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3646
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.314"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(359..1963)
                     /locus_tag="ACHKBL_004638"
     CDS             complement(359..1963)
                     /locus_tag="ACHKBL_004638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001083419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfjI family protein"
                     /protein_id="HFO5821384.1"
                     /translation="MNPDYVQALQTSPHFPTDVTYRLFPSDLAYLIDDLYSSTQLPLE
                     LIFNTVLATLSLSCQSLVDVVHPHTNMPEPCSLYLLAIAEPGAGKTTINRLVMTPCYE
                     FADRLIQQYEERNKDYKTELQIWNTRQKALAANLRKAVNRGDPGEQEEEALRNHERNK
                     PTRPVRPNFIYEDVSLKALAEGLNEHPEAGIISDEAVTFFRSYLKNNPGLLNKAWSGQ
                     PFDFGRADEKYHITPRLTFSLMSQPNVFTDYINKNDVLAWGSGFLSRFLFSQVGGSSR
                     VRDYRKGEFVTIPTLVEFHKKINGFLSSHSINSPGMRSERKTLKLSKKALGEWQENQI
                     KIESKALAGGEWEHIRDIVLKAGSNILRIAGLFTCYCYKDDEEITSIALWKAMHLMEW
                     YLEEASTIFYPMSERCQFEQDARELYTWIMTRIKQNNLYAIRKTDIERYGPNRLRRAE
                     KLTPVLNQLIAQNYLCIIKMRSHQALYVSVHCNNRYLLPCGAMSYEQFDIVPPLNSHN
                     AKTYHVNIPLALIPSFALPFSAYTLF"
     gene            complement(2393..3298)
                     /locus_tag="ACHKBL_004639"
     CDS             complement(2393..3298)
                     /locus_tag="ACHKBL_004639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFO5821385.1"
                     /translation="MALEQEIANLVNDFVAGGRPSCRDQTFDERRAGYIASTALAGDT
                     ETRVNISDVIIDDILLRIFSPLNDDKLSSAIVYYHGGCFVSGGFVTHDNQFRQLAFLS
                     GCRVIAVQYRLAPEHIYPAAHDDAEHAANIVWQHAEMLKVDRNKITLMGDSAGGHLAL
                     ITALRVKNIAQWLPRKLILIYPMLDATASCDSYKLNGSDYVLTEDTFLSGYEAYFSGL
                     DLEHPEASPLFRKDFNGLPPVHIITAEYDPLRDEGEELFSRMIQQGVKCTAQRYLGVI
                     HGFFQLGGISQTARNLMQDICGIVE"
     gene            complement(3428..>3646)
                     /locus_tag="ACHKBL_004640"
     CDS             complement(3428..>3646)
                     /locus_tag="ACHKBL_004640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5821386.1"
                     /translation="VIYCLSLVRKSRSLKLRETLNAVIASTEGDMCRQQKESFAALTQ
                     HKVSIDAFLCGCTVTCARTKTSLSILQQ"
BASE COUNT         1069 a          817 c          719 g         1041 t
ORIGIN      
        1 atcaaacaaa gttatatata aaaactcaca taatatggtt aataattagc ttcaatccat
       61 gtgaaaaata acatttcgat tcactttcga gatatcaaaa acgaaaacaa ttcttccacc
      121 agtgcacaca ttcctgcatt caaccagctc tgacaacagg tcattatgaa aacaaaatca
      181 aatcacatac atggtacatg tactgctacc gttgctaccg tagcgatttt ttcagagaaa
      241 ttttcgttca caccccataa ttggtgaata tttgcacaaa tgcaggatat gaaaaacgcc
      301 ttaccatcaa ccttcgaatg aaggcttatg tgttttgttt gcttattgta aagctgtttc
      361 aaaataaggt ataggctgaa aaaggtaacg caaatgacgg aattaatgcc agagggatat
      421 taacgtgata tgttttcgca ttatgactat tcaagggtgg aacaatatca aactgttcgt
      481 aagacattgc accgcaagga aggaggtaac gattgttaca atgaacagaa acataaagtg
      541 cctgatgcga tcgcattttg atgatacata aataattctg tgcgattaac tgattgagta
      601 caggtgtaag tttttctgct cttctcagac gattggggcc atatctctca atgtctgttt
      661 tcctgatagc atacaaatta ttctgcttga ttcgggtcat aatccatgta tacagttcac
      721 gggcatcctg ttcaaactgg catcgttcag acataggata aaatattgtg cttgcctcct
      781 ccagatacca ctccatgaga tgcatcgctt tccaaagcgc tattgatgta atttcctcat
      841 cgtctttata gcaatagcag gtgaataaac ccgctatcct cagtatatta gaacctgctt
      901 tcagaacaat atctctgatg tgttcccatt ccccacctgc aagcgctttg ctttcaattt
      961 taatctggtt ttcctgccac tctcccaacg ctttcttcga aagttttaat gtcttccttt
     1021 cgcttctcat tccgggggaa ttaatgctat ggcttgacag aaacccgtta atctttttat
     1081 gaaactccac cagggttggt attgttacga actcgccttt cctataatca cgtacccggg
     1141 aagagcctcc aacctgactg aaaagaaaac gggaaagaaa tccgcttccc catgccagta
     1201 catcattttt atttatataa tccgtaaaaa cattcggctg agacattaat gaaaatgtca
     1261 ggcgtggagt aatatgatat ttttcgtctg ctctcccaaa atcaaacggt tgtccactcc
     1321 atgctttatt caacagaccc ggattatttt tcagatagct tctgaaaaaa gttaccgctt
     1381 catcagaaat aatccctgcc tccggatgtt cgttgagtcc ttccgcaagc gctttaagcg
     1441 aaacatcttc atagataaaa ttcggtcgaa ccggacgtgt cggtttattt ctttcgtgat
     1501 tacgcagtgc ctcttcttcc tgttctcccg gatccccccg gttaacagcc tttcttaaat
     1561 tggcagcaag agctttctgc cgggtattcc agatctgtag ttcagtctta taatctttgt
     1621 ttctctcttc gtattgttga atgagtcgat cggcaaattc gtaacagggg gtcatcacca
     1681 gtctgtttat cgttgttttt cccgcgcctg gctctgcgat tgccaacaga taaagtgagc
     1741 agggttccgg catgttagtg tgaggatgaa caacgtcaac caatgactga caggagagtg
     1801 agagcgttgc cagtacagta ttaaaaatga gctccagagg aagttgggta cttgaataca
     1861 agtcgtcaat gagatacgca agatcactag gaaataatct gtaggttaca tctgttggga
     1921 agtgggggga tgtctgcaag gcttgcacat aatcgggatt cattgtaatg gacctccttc
     1981 acatactgta atcagcacaa agcttattgt tcagttcaga aacataccaa cattgtgagt
     2041 atgttcgctg acgccaataa taaaatgact gatctccatt caccagtccc ggcgcattta
     2101 tggttctgac aaaagtaaac aacatgggag ccagaactct gcatcgcagc gacaaactat
     2161 ggatgcatga aaaacaggga acattacgca ccagagcgtt gagagtcttc ctccttgccc
     2221 aatacgaaag tcatctatcc acccgtcttc aatcttcggg atggttgttt tatgaacaga
     2281 gtaaaagttt gaagcaagct gagaacctct gtttccacgt aaaggtccgc tatgaagaaa
     2341 aatgggagtt tgcatgctca actaatttac ctgtaggtgc ttgttactgt agttattcaa
     2401 caatcccaca aatatcctgc atcagattac gggctgtctg gctaatgccg ccaagctgga
     2461 aaaaaccgtg aatgacaccg aggtaacgct gggcggtaca ctttactcct tgttgaatca
     2521 tacgggaaaa cagttcctca ccttcatcgc gtaatgggtc atattccgct gtgatgatat
     2581 gaacaggtgg taacccattg aaatcttttc tgaataatgg actggcttcc ggatgttcta
     2641 aatccagacc ggaaaaataa gcctcataac cactaagaaa cgtgtcttct gtcagcacat
     2701 aatcacttcc gtttaatttg taactatcgc atgatgctgt tgcatccagc atagggtaaa
     2761 taagtattaa ttttcgtggt aaccactgag cgatgttttt cacccgcagt gctgttatca
     2821 gtgcaagatg tccccctgcg ctatctccca ttaacgttat tttgttgcga tcaactttca
     2881 acatttctgc gtgttgccat acaatattag cggcatgttc agcatcgtca tgtgccgcag
     2941 gataaatatg ttccggcgct aatcgatatt gtacagctat gacacgacag ccgctcagga
     3001 aagcaagctg tcgaaactga ttatcatggg ttacaaaacc accactgaca aaacagccac
     3061 catgataata gacaattgcg gatgacaatt tatcatcgtt taaaggcgaa aagatacgca
     3121 ataaaatatc atcaataatc acatcactga tgtttacgcg ggtttctgta tcgccggcca
     3181 gagcagtact tgcaatatat cccgcccgac gttcatcaaa tgtctgatca cgacaggatg
     3241 gacgaccacc cgcaacaaaa tcatttacaa ggtttgcgat ttcctgttca agtgccatac
     3301 aaatcaactc cgaccgataa tatttaaaca acgctacggg aagtataaat actctgacca
     3361 aaatataaca ggtgaatatc catagcttca acaggcgtaa tcaataaggg cgtacggagc
     3421 ttgcccgtta ttgctgcagg atcgacagac tggttttcgt ccttgcgcat gtaacagtac
     3481 aaccacagag aaaagcatca atggacactt tatgttgcgt cagggcagcg aacgactcct
     3541 tttgttgacg acacatatca ccttctgtac tggcaattac ggcattcaga gtctccctga
     3601 gttttaagct cctgctctta cggaccagcg acaagcaata aataac
//
LOCUS       DAZFLD010000313         3604 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.315, whole genome shotgun sequence.
ACCESSION   DAZFLD010000313 DAZFLD010000000
VERSION     DAZFLD010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3604
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.315"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(126..1517)
                     /gene="narK"
                     /locus_tag="ACHKBL_004641"
     CDS             complement(126..1517)
                     /gene="narK"
                     /locus_tag="ACHKBL_004641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate transporter NarK"
                     /protein_id="HFO5821387.1"
                     /translation="MSHSSAPERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPC
                     LLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFGGR
                     RWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPK
                     QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWV
                     WVPFLAIFTIAAWFGMNDLATSKASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAG
                     FAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTRVTLVNFILMAIFSGL
                     LFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSD
                     ERAMREAATDTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACV
                     VITWAVYGRHSKK"
     gene            1856..>3604
                     /gene="narX"
                     /locus_tag="ACHKBL_004642"
     CDS             1856..>3604
                     /gene="narX"
                     /locus_tag="ACHKBL_004642"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarX"
                     /protein_id="HFO5821388.1"
                     /translation="MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSA
                     HAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQTAFSAELTRAAERDGQLAQLQGL
                     QDYWRNELIPALMRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMA
                     VFMALLLVFTIIWLRARLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNN
                     MSAELAESYAVLEQRVQEKTAGLEHKNQILSFLWQANRRLHSRAPLCERLSPVLNGLQ
                     NLTLLRDIELRVYDTDDEENHQEFTCQPDMTCDDKGCQLCPRGVLPVGDRGTTLKWRL
                     ADSHTQYGILLATLPQGRHLSHDQQQLVDTLVEQLTATLALDRHQERQQQLIVMEERA
                     TIARELHDSIAQSLSCMKMQVSCLQMQGDALPESSRELLSQIRNELNASWAQLRELLT
                     TFRLQLTEPGLRPALEASCEEYSARFGFPVKLDYQLPPRLVPSHQAIHLLQIAREALS
                     NALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRAQSLRGD
                     CRVRRRESGGTEVVVTF"
BASE COUNT          967 a          940 c          924 g          773 t
ORIGIN      
        1 aacatattaa tggctcttaa tgaaaaccgg cgaaatacgc cacacttcat aaggatgttg
       61 taactaaatg acaggtaaaa aaagcgcgac atcatgccgc gcaaaggata atcaaatatt
      121 aagatttatt ttttagaatg ccgaccatat accgcccagg taatcactac gcaggcgata
      181 tagaaaatca aaaatacttt cattgcgccg actggcgaac ccgttaatgc cagcgagcta
      241 ccgaacgctt tcgggataaa gaagccacca atcgcgccaa tcgcagagat gaaacccagc
      301 gccgccgccg tgtcggttgc cgcttcacgc atcgcacgtt cgtcagagcc cccttctgct
      361 ttcacgcgat ccattgtcag tttacggaag atcacggaaa tcatctgaaa agtggaacca
      421 ctacccagcc cagctgtcag gaacagcgcc aggaagactg cgaagaacgc catgaagctt
      481 ccgccctgtc cgtcagtcgg taaggtcagg aacagcaggc cgctgaaaat cgccatcagg
      541 ataaagttca ccagtgtgac acgagttccg cccagacggt cagataatgc accacctgca
      601 gaacgcgcca gcgcaccaat aaacggcccg aagaaagcgt attgcagaat ctgaacatcc
      661 gggaactgcg tttttgacag catcgcaaag cccgcggaga agccgatgaa ggagccgaag
      721 gttgccagat acagcaggct cataatccac agatgacccc gtttgagtac cggcaactgc
      781 tccttgatgg aggctttcga ggtagcaaga tcgttcatgc caaaccacgc cgcaatggtg
      841 aagatggcaa ggaacggcac ccatacccag gacgcattcg ccagatacag ctcagtccca
      901 tccggctgtt tgacaccctg gctaccaaat actgcgaaaa tcgacagtga taccaccagc
      961 ggagcaacca actgcatgac gctgacgccc atgttgccca gaccaccatt cagacccagc
     1021 gcgccaccct gcttctgttt cggaaagaag aagctgatgt ttgccatact ggatgcgaag
     1081 ttcgcgccag caaagccgca cagcagagag atgatgatga agacgctata aggcgtggag
     1141 gtatcctgca cggcaaaacc cagccagacg caaggaataa tcagaatacc agtgctgaac
     1201 gccgtccagc gacgaccacc gaagatagga accataaagg agtatggaac acgtaataac
     1261 gcgccagaaa ccgaaggcag cgccgtcaac ataaatagct gatcggtcgt aaaattaaaa
     1321 ccgactttcg gtaagttcac cgcaacagcg ctgaacaaca tccatacgca aaacgccagc
     1381 agcagacagg gaacggaaat ccacaggttg cggctggcaa tacgttgacc gcgttgttgc
     1441 cagaacgcag gatcttccgg tcgccaatct gtaatgacag ctccagtagc cctttcgggg
     1501 gcggatgagt gactcataga cacctctgat actcgtttcg atgtctgcca ccttagtgtc
     1561 tgtagctaaa ggcaatttga tgtaaatcaa acgataagat aactttatct atcattgata
     1621 tttatcatta cccatagtga gtacagtgat ttcataaaaa ttatgagatt tttcacggtg
     1681 ctgtaaaatc cctaccctta ccgatgtaaa gcgactaacc acgcggcaaa taaggagtaa
     1741 ctctttccgg gtatgggtat acttcagcca atagccgaga atactgccat tccagaatgt
     1801 atcgtcacat tcattaaggt tattgctcat ttaaagcctg aaggaagagg tttgcatgct
     1861 taaacgttgt ctctctccgc tcaccctggt taatcaggtt gcgcttattg tgttgctttc
     1921 tactgctatt ggactggcag ggatggcagt ttctggctgg ctggtgcaag gcgttcaggg
     1981 cagcgcccat gcgatcaaca aagcgggatc gctgcgcatg caaagttacc gtctgttggc
     2041 agcagtgcca ttaagcgaga aagacaagcc cttaattaaa gagatggaac aaacagcatt
     2101 tagcgccgag ttaacccgag cagcagaacg agacggacaa ctggcgcaat tacagggttt
     2161 acaagattac tggcgtaatg aactgatccc tgcgctaatg cgtgcacaaa accgcgaaac
     2221 ggtgtcagcg gatgtcagcc agtttgttgc cgggcttgat caactggtat ctggttttga
     2281 ccgcaccacg gaaatgcgca ttgagacagt ggtgctggtc catcgggtaa tggcggtatt
     2341 tatggcactt ttactggtgt tcactattat ctggttgcgg gcgcgactgc tacaaccgtg
     2401 gcggcagttg ttggcaatgg cgagtgccgt cagccatcgc gattttaccc aacgcgcgaa
     2461 catcagcggg cgcaacgaaa tggctatgct tggaactgcg ttgaacaata tgtctgcaga
     2521 actggcggaa agttacgccg tacttgagca gcgggttcag gagaaaaccg ccgggctgga
     2581 acataaaaat cagatcctct cttttttatg gcaggccaac cgccgtttgc attcccgcgc
     2641 cccgctgtgt gaacgcctgt cacctgtact caacggctta cagaatttaa ccctgctacg
     2701 tgatatcgaa ctgcgggtgt atgacactga tgatgaagag aatcatcagg agtttacctg
     2761 ccagccagat atgacctgcg atgataaagg ctgtcagctc tgcccgcgcg gtgtactccc
     2821 cgttggcgat cgcggcacaa ccctgaagtg gcggctggct gactctcata cgcagtacgg
     2881 tattttgctg gcaaccctgc cgcaggggcg tcatcttagt catgatcaac aacaactggt
     2941 ggataccctg gttgaacaac tcaccgccac gcttgcgctg gatcgccatc aggaacgtca
     3001 gcaacagttg atcgtgatgg aagagcgtgc caccattgcg cgcgaactgc atgattctat
     3061 tgcccaatct ctctcttgca tgaagatgca ggtgagttgt ttacagatgc agggcgatgc
     3121 gctgccagaa agcagccgcg aactgttaag tcagatccgt aacgaactga atgcatcctg
     3181 ggcgcagttg cgtgaattgc tcaccacatt ccgcttgcag ctcaccgagc ctggattacg
     3241 tccggcgctg gaggcgagtt gcgaagagta cagcgccagg tttggcttcc cggtgaagct
     3301 ggattatcaa ttgccgcctc gtctggtgcc ttcgcatcag gcaatccatc tgttgcaaat
     3361 tgcccgtgag gcattaagta acgccctcaa acattcgcaa gcgagtgagg tcgtggtgac
     3421 agtggcgcaa aacgataatc aggtcaaact gaccgtccag gataacggct gcggcgtgcc
     3481 tgaaaatgcc atccgcagca atcactacgg catgataata atgcgcgacc gtgcgcaaag
     3541 tttacggggc gattgccgcg tccgccgtcg tgaatcaggt ggcaccgaag tggtggtcac
     3601 cttt
//
LOCUS       DAZFLD010000314         3598 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.316, whole genome shotgun sequence.
ACCESSION   DAZFLD010000314 DAZFLD010000000
VERSION     DAZFLD010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3598
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.316"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(107..1426)
                     /locus_tag="ACHKBL_004643"
     CDS             complement(107..1426)
                     /locus_tag="ACHKBL_004643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HFO5821389.1"
                     /translation="MDICSRNEKLAIRRPAILVALALLLCSCKSTPPESMVTPPTGSK
                     PPATTQQSSQPMRGIWLATVSRLDWPPVSSVNISNPTSRARVQQQAMVDKLDHLQRLG
                     INTVFFQVKPDGTALWPSKILPWSDLMTGKIGENPGYDPLQFMLDEAHKRGMKVHAWF
                     NPYRVSVNTKPGTIRELNSTLSQQPASVYVQHRDWIRTSGDRFVLDPGIPEVQDWITS
                     IVAEVVSRYPVDGVQFDDYFYTESPGSRLNDNETYRKYGGAFASKADWRRNNTQQLIA
                     KVSHTIKSIKPEVEFGVSPAGVWRNRSHDPLGSDTRGAAAYDESYADTRRWVEQGLLD
                     YIAPQIYWPFSRSAARYDVLAKWWADVVKPTRTRLYIGIAFYKVGEPSKIEPDWMING
                     GVPELKKQLDLNDAVPEISGTILFREDYLNKPQTQQAVSYLQSRWGS"
     gene            complement(1557..3092)
                     /gene="gadC"
                     /locus_tag="ACHKBL_004644"
     CDS             complement(1557..3092)
                     /gene="gadC"
                     /locus_tag="ACHKBL_004644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance gamma-aminobutyrate antiporter
                     GadC"
                     /protein_id="HFO5821390.1"
                     /translation="MATSVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVF
                     FLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIG
                     FIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
                     FAGILLPAFILIALAAIYLHSGAPVAIEMDSKTFFPDFSKVGTLVVFVAFILSYMGVE
                     ASATHVNEMSNPGRDYPLAMLLLMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFT
                     VLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNK
                     NGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVL
                     VLKHPDLKRTFNIPGGKGVKLVVAIVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLV
                     VSFLVVLALPFILYAVHDRKSKANTGVTLEPINSQNAPKGHFFLHPRARSPHYIVMND
                     KKH"
     gene            complement(3248..>3598)
                     /locus_tag="ACHKBL_004645"
     CDS             complement(3248..>3598)
                     /locus_tag="ACHKBL_004645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HFO5821391.1"
                     /translation="AYLADEIAKLGPYEFICTGRPDEGIPAVCFKLKDGEDPGYTLYD
                     LSERLRLRGWQVPAFTLGGEATDIVVMRIMCRRGFEMDFAELLLEDYKASLKYLSDHP
                     KLQGIAQQNSFKHT"
BASE COUNT          939 a          890 c          902 g          867 t
ORIGIN      
        1 tgcgtagtta atttgactta agtaagcatg attactagtg ggatatttca agagggtaac
       61 aagccggggg gaaagcaccg gcttgttaca aagtaagaat gggagtttaa ctgccccaac
      121 gactttgcag atagctgacc gcttgttgag tctgtggctt attcagatag tcctcacgga
      181 acaagattgt gccgctaatt tctggcacgg catcgttaag atcgagctgc tttttcagtt
      241 ccggtacgcc gccgttaatc atccagtctg gctctatctt tgaaggttca cccactttat
      301 agaaggcgat accgatatac aggcgggtcc tggtcggttt aacgacatcc gcccaccatt
      361 ttgccaaaac gtcataacgt gcggcactcc gtgagaacgg ccagtaaatt tggggagcaa
      421 tgtaatccag caatccttgt tccacccatc gacgggtgtc agcgtaggat tcgtcatagg
      481 ctgccgcgcc tcgggtatcg gaaccgagcg gatcgtgtga tcggttacgc cacacgcctg
      541 ccgggctaac gccaaattcg acttccggct taatgctttt aatagtgtgc gatacctttg
      601 caattaactg ctgagtattg ttgcgccgcc agtctgcttt tgacgcaaat gcgcctccgt
      661 atttgcggta cgtttcgtta tcatttagcc gtgaacccgg tgactcagta tagaaatagt
      721 cgtcaaactg cacgccatct accggatagc gggaaaccac ttccgcgact attgatgtga
      781 tccagtcctg aacttcaggg atgcccgggt cgaggacaaa gcgatcgccg gacgttctga
      841 tccagtcgcg gtgttgcaca tagacgctcg ctggttgttg agacagagtg ctattcagtt
      901 ccctgatagt accgggcttc gtattaaccg atacgcgata ggggttaaac caggcgtgta
      961 ctttcatccc acgcttgtgg gcttcgtcga gcataaattg cagcggatcg taacctggat
     1021 tttcaccaat ctttccggtc ataagatcgg accacggcaa aatttttgat ggccacaggg
     1081 cggtaccgtc cggcttgacc tggaaaaaga ccgtgttgat gccgagacgt tgcagatgat
     1141 ccagtttgtc gaccatcgcc tgttgttgta cacgggcccg gctggtgggg ttactaatgt
     1201 taaccgagga aaccggtggc cagtcgaggc gagacactgt ggccagccag atgccacgca
     1261 tcggttgtga cgattgttgc gtcgtagctg gtggcttcga acctgttggt ggtgtcacca
     1321 ttgactctgg aggcgtgctt ttgcaactac acagtaaaag tgccagtgca accagtatcg
     1381 ctggtcttct aatcgctaat ttctcgtttc gggagcagat atccatatgt gctggtttcc
     1441 ggtaaacaga tgtgcgctca ttctcgtact tatcccggtt aagtcaatac gacaacaagc
     1501 acgaaaaagg gagcgatgaa ttatcgctcc cttgtcttat aaccattcag acatggttag
     1561 tgtttcttgt cattcatcac aatatagtgt ggtgaacgtg cacgcgggtg caggaagaag
     1621 tgaccttttg gtgcgttctg actgttgatt ggctccagag tgacgccggt attcgctttg
     1681 cttttacgat catgaacagc atagagaata aagggcaggg caagtaccac caggaaactc
     1741 acgaccagta attcaacata catatcggta gagtcaccct ggatgttatc cggcggcagg
     1801 aaggaaacaa taaacgccat aattgaagtc agcagaccga caatagccac gaccagtttc
     1861 acccctttac caccagggat attaaatgtg cgttttaagt caggatgttt aagaaccaac
     1921 acaatgtagc caataaacag catgaaataa gcacacagat aaatcaccac cgtcagcgcc
     1981 agtgcgatca ggaaggacat gttgttaccg ccaccggtat tggtgaggat gatcaacgcg
     2041 atagaagtaa tcaccaactg cgaaatgacc agcgttaccg gtacgccatt tttgttcatt
     2101 ttagcgaatg ccgctggcag caggtttttc tgcgctgtga catacatccc gcgagaagga
     2161 ccaacaatcc aggaagcgat ttccgccaga acacccagca acagcagtgc ggagatcacg
     2221 cgaaccgtcc actcaatttc cggtgccaca tgtgacatca gaacggtaaa ggtttgcatt
     2281 acccctgcgg agaggttgat ttcattaccc ggaatgacca tcgcaataga caaaccgcca
     2341 acagagctta agcagattgc cgccaccatc agcagtaaca tagccagtgg atagtcgcgc
     2401 ccgggattgc tcatttcatt gacgtgggtt gcggaagctt caacgcccat ataactcaaa
     2461 atgaaggcaa caaatacaac cagggtgccc actttagaga agtcagggaa gaaggtcttc
     2521 gaatccattt cgatagcaac gggggcaccg gagtgcagat aaatagccgc taatgcgatc
     2581 aaaataaatg caggtaacag gataccggcg aagaagccaa ctttagcaat tcgcgccgtg
     2641 tatttcgtgc caccaaactg cgttaatgcc agcgcccaaa gaatgatgag tgctgcaata
     2701 gttttggtaa tggggtcttc attcagcgct ggccatttca ggatgtagga gagtgcccct
     2761 aacacgaaat agagcatcgg aataaaacca atggcgattt gcagatagcc aaatgagatc
     2821 gctgcaaatc cccatctcgg ccccagagta tttgataccc aggcgaagac gccaccttct
     2881 tcccagccgt cgacggtagc catttccgca gcacaaagtc ccacgggaat aaaccataaa
     2941 atcccgccta atagcaggaa gaagactaat gaaaagcccg atgttgcgaa ggtagggtat
     3001 tcataaacag ccattaccat cgatgccgtt atggcaaaaa atccgagtaa tgtgagctgc
     3061 ttagctttac ctgtctgtac tgatgtagcc atattatccc cctaaaacgg tattcctgtc
     3121 ggaaccgcac ctgttttgtt ctgaagcgta ttcagaacaa tattttccgt tgctaatgcc
     3181 agtgaacaga ctttggaaat tgtcccgaaa cggcttcgtt tcgggacacc gttaccgtta
     3241 aacgttatca ggtgtgttta aagctgttct gctgggcaat accctgcagt ttcgggtggt
     3301 cgctgagata tttcagggag gctttgtagt cttccagcaa cagttcagca aagtccattt
     3361 cgaagccgcg acggcacata atgcgcatca ccacgatgtc ggtggcttca ccgcctagag
     3421 tgaaagccgg aacctgccag ccgcgcagac gcaaacgttc agagaggtca tacagggtgt
     3481 atcccggatc ttcaccatct ttcagtttga agcaaaccgc cgggatgcct tcgtccgggc
     3541 gacccgtaca gatgaactca tacggcccca gtttggcgat ttcatccgcc agataagc
//
LOCUS       DAZFLD010000315         3557 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.317, whole genome shotgun sequence.
ACCESSION   DAZFLD010000315 DAZFLD010000000
VERSION     DAZFLD010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3557
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.317"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            459..1025
                     /locus_tag="ACHKBL_004646"
     CDS             459..1025
                     /locus_tag="ACHKBL_004646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001126806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HFO5821392.1"
                     /translation="MPKSYTPNWFFTALLDNHINQMLARYSCLRALRMDFFYRKDTPD
                     FLQPDHRWLELQLRMLLEQVEQFENIVGFFWVIEWTADHGFHAHAVFWIDRQRVKKIY
                     PFAERIAECWRSITHNSGSAHRCTYQPHYTYNINIPVRHNDPESIDNIRGALHYLAKE
                     EQKDGLCAYGCSEVPERPAAGRPRNLQK"
     gene            1283..1858
                     /locus_tag="ACHKBL_004647"
     CDS             1283..1858
                     /locus_tag="ACHKBL_004647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5821393.1"
                     /translation="MNAIPYFDYSLAPFWPSYQSKVIGVLERALREQSGSRIRRILLR
                     LPCEYDNTFNSRTTWFGMDFIETVSALMNAKPGRDLCWLLTRHPEKPEYHVVLCVRQE
                     YFDGPELDRLILDAWSNVLGFASPGEAAPYQKQITRDVLLDSCSPDCEDILKDLIWAF
                     SDFARDRRGVCDPEARCLAGNPGYPGSAGLF"
     gene            1927..2163
                     /locus_tag="ACHKBL_004648"
     CDS             1927..2163
                     /locus_tag="ACHKBL_004648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000958092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO5821394.1"
                     /translation="MLTSITGHDSILLRADDPLIDMNYITSFTGMTDKWFYKLISEGH
                     FPKPIKLGRSSRWYKSEVEQWMQQRIEESRGAAA"
     gene            2445..2957
                     /locus_tag="ACHKBL_004649"
     CDS             2445..2957
                     /locus_tag="ACHKBL_004649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001680714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5821395.1"
                     /translation="MQITKALISEPGDIRRFVQQAVDHWPNLLAFHFTLYSAEGNING
                     QQIHAFCTSFYRQVHERITERNHTASPSSPVVLRWLREQHRGATIRCLLLLSQTSICH
                     PRASVTVDEECSQLVDLLQQTWKVISAGGQCRVEKRFQVVRGDTSGQYVALKTVVQSF
                     VLPVMTTINW"
     gene            3046..>3557
                     /locus_tag="ACHKBL_004650"
     CDS             3046..>3557
                     /locus_tag="ACHKBL_004650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFO5821396.1"
                     /translation="MRTRIDYLANKYSFTELNESPRLRWQWQDVLEECRQTEAGPEER
                     LRIALLNVDYVTSFELPFRLLLIRTPQLIAALREEWDISQKNVMFNDKRFGCVYSLKA
                     SLSGVPDTYRYHLSHRIRRVVGNESTSSPYQQVAREVKAPRERLKYALEAGLLVTALD
                     GLFWSGSQRIA"
BASE COUNT          848 a          846 c          870 g          993 t
ORIGIN      
        1 gagttgtttt taaccgatta aatgagtgcg tgtaagttta ttgggttaca ggtatatgtt
       61 ggccttgtac acgttttttc agactcactg agagcaagtt tgtagtttat gatgttattg
      121 gtcgtaatat gttttttgaa ctattggtcg taatatgttt tttgaactat tggtcgtggg
      181 tttttatcgc gtgttttgca gtatttcgtg atgtatgttt attgattctg tattttattc
      241 gctcctcgtt tatctcatct ctttagctaa taccatcaga taatccattt ctttctgcat
      301 aatgctgcgt atcgttaata acccgtcgta tccattctgc tacagcatgc ctgataaata
      361 ccatctgtaa gttattaccg ttttagatct gattatgagc gaaagcatta attcgttcac
      421 agagcttaaa acatcattaa ctttcaggag tcatcaacat gcctaaatct tacacaccaa
      481 actggttttt taccgcttta cttgacaatc acatcaatca aatgctggca cgttattcct
      541 gcctgcgagc tttacggatg gatttcttct acaggaaaga tacgcctgat ttcttacaac
      601 ctgatcatcg ctggcttgaa ttgcagttgc gtatgctgct ggagcaggtg gaacaatttg
      661 aaaatatcgt tggcttcttc tgggtgattg aatggacggc tgatcatggt tttcatgcac
      721 atgcggtttt ctggatcgat cgtcagaggg ttaaaaaaat atatcccttt gcggagcgga
      781 tagctgaatg ctggcggtct attacgcata acagcggttc ggcacaccgc tgtacatatc
      841 agccgcatta tacatacaac atcaacattc ctgtgcgcca caacgatcct gaaagcatcg
      901 ataatattcg cggtgccctg cattatctgg cgaaagaaga acaaaaagac gggctgtgtg
      961 cctacggctg cagtgaagtt cctgagcgtc ctgccgcagg gcgtccacgt aatcttcaga
     1021 agtgaagctt aaaccttgag tttcctggcc cggaaacact ccgttagtaa aaacttaccg
     1081 ccctgcttaa tgatgtgaac cgaagtcaat ggagatcatt caccctgaac cacatccggt
     1141 gttttgttcc ttgtcagtta cctgatcttc ccgttttgcc ccggtttttc acttattcca
     1201 tcaaccccat ccgcaaacca taacacgtca gcttattcac gggcaggacg cattgtgggc
     1261 tgcgcataat ggagcatatc ttatgaatgc tatcccttat ttcgactata gcctggcacc
     1321 cttctggcca tcttatcaga gcaaagtcat cggcgttctt gagcgtgcgc tgcgtgagca
     1381 gtccggctca cggatacggc ggatcctgct tcgtctgccg tgtgaatatg acaatacctt
     1441 caacagcaga acgacctggt ttggtatgga ttttatcgaa accgtcagtg cgctgatgaa
     1501 tgcgaaaccc ggacgcgacc tttgctggct cctgacccgt catcctgaaa agccggaata
     1561 ccacgtggtg ctgtgtgtca gacaggagta tttcgacggc cctgaactgg atcggctaat
     1621 tctggatgcc tggagcaacg tactgggctt cgcgtcaccg ggtgaagcag caccgtacca
     1681 gaagcagatc accagggatg tactgctgga cagttgttca ccggactgcg aagacatcct
     1741 caaagacttg atctgggcgt tcagtgattt cgcccgcgat cgtcgtggtg tgtgcgatcc
     1801 ggaagcccgt tgcctcgccg gtaatcccgg ctatcccgga tctgcaggac tgttctgaaa
     1861 gctcagttca gcagattcac cagcatttgc ccaacctcca taaatctcac agaggacatc
     1921 ctgactatgt tgacctcaat aacaggccac gacagtatat tgctgcgtgc cgacgatccc
     1981 ctgatcgaca tgaactacat caccagtttc accggtatga ccgataaatg gttttacaag
     2041 ctgatcagtg aaggtcattt tcctaaaccc ataaaactgg gtcgcagcag ccgttggtac
     2101 aaaagtgaag tggagcagtg gatgcagcag cgaattgagg aatcacgagg agcagctgca
     2161 tgaaacgtgt tgtgatgccg gtacgctggc aatgtgcaaa atgtcggcgc tggtattgtg
     2221 gagatcagcc ctgctcctgg tgctggcgct gacaactccc gctcatcttt ctgctgactc
     2281 ctctggtcag ccaactgttt gtcatcattt cctgactgat tcgtcattcc cttcgtcccg
     2341 ccagtcactg gtgtttgtat cgtatcgtgc cggtgttgat gtgcgctttc cgtgtgtctg
     2401 caccggctta acaaaattca acagggtttg aaaaggaaca tttcgtgcaa ataaccaaag
     2461 ccttaatttc agagccggga gacatccggc gttttgttca gcaggcggtg gaccactggc
     2521 caaatctgtt ggcatttcac ttcacgctct attcggcaga aggaaacatc aacgggcaac
     2581 agattcatgc attctgtact tccttttacc gacaggtgca cgaacgtatt actgagcgta
     2641 atcacacggc cagtccatcc tcgccggtgg tattacgctg gttgcgggaa caacatagag
     2701 gagcaacaat tcgatgcctg ttgctgctca gccagacgag tatttgtcac ccgcgagcca
     2761 gtgtcacagt tgatgaagaa tgttcacaac tggtggattt actacaacag acctggaagg
     2821 tgataagcgc tggcggacaa tgccgggtgg aaaagcgttt tcaggtcgtc cggggtgata
     2881 catctggcca gtatgttgcg ttaaaaacag tcgttcaatc ctttgtgtta ccggttatga
     2941 caacaatcaa ctggtgatgg tcgtgtgaac cagctgcgct tccgttgatt cttatggact
     3001 gataaagttt tgtcagatga atttttatac agaattcttt tcagtatgcg tacacgaatt
     3061 gattatctgg cgaataaata cagttttaca gagctgaatg aatccccccg tcttcgctgg
     3121 cagtggcagg atgttctgga ggagtgccgg cagacagagg ccgggccaga agaacggctg
     3181 cgtattgctc tgctgaatgt ggattacgtc accagttttg aactgccttt tcgcttgttg
     3241 cttatccgta caccacaact gattgccgcg ctgcgtgaag aatgggacat cagccagaaa
     3301 aatgtgatgt tcaacgataa acgctttggc tgcgtgtaca gcctgaaggc cagcctttcc
     3361 ggtgtgccgg atacataccg gtatcatctg tctcaccgta ttcgtcgcgt ggttggaaat
     3421 gaaagcacat catcgccata tcagcaggtt gccagggaag tgaaagcgcc ccgtgaacgg
     3481 ctgaagtatg cgctggaggc gggcttactg gtgaccgcac tggacggcct tttctggtct
     3541 ggcagtcagc gtattgc
//
LOCUS       DAZFLD010000316         3505 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.318, whole genome shotgun sequence.
ACCESSION   DAZFLD010000316 DAZFLD010000000
VERSION     DAZFLD010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3505
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.318"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..400
                     /locus_tag="ACHKBL_004651"
     CDS             <1..400
                     /locus_tag="ACHKBL_004651"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO5821397.1"
                     /translation="VTQDIRRRIAACFVAIDNDHRIIGYYTLSAASILLTDLPEHLRK
                     KLPRYPTIPAIRMGRLAVSLSSKGTGLGGTLLADALYRAYTSEIAAYALIVDAKDDTA
                     VSFYKHYGFMTLPESGRTLFMPLASLSAIE"
     gene            complement(487..639)
                     /locus_tag="ACHKBL_004652"
     CDS             complement(487..639)
                     /locus_tag="ACHKBL_004652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001618166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFO5821398.1"
                     /translation="MPQRTFLTMLIVVCVTILCFVWMVRDSLCGFRVEQGNTVLVATL
                     AYEVKR"
     gene            969..1211
                     /locus_tag="ACHKBL_004653"
     CDS             969..1211
                     /locus_tag="ACHKBL_004653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5821399.1"
                     /translation="MKDEIQSMCLTPDIVKQFNTLLNTHCQSSEEALGMLSALCFELM
                     MKCGVQSINSLMVNGMGISMQLQGIDIAERPGKNIH"
     gene            1307..1498
                     /locus_tag="ACHKBL_004654"
     CDS             1307..1498
                     /locus_tag="ACHKBL_004654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5821400.1"
                     /translation="MKKIKTHTGLLASKDKNWRVSLYETPTAWCIRGQECYSKSTGRR
                     CGSHDSLSRLLLDSIKPVE"
     gene            1510..1878
                     /locus_tag="ACHKBL_004655"
     CDS             1510..1878
                     /locus_tag="ACHKBL_004655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001456887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5821401.1"
                     /translation="MKDNNTTATPEAWKMRFLANGTSGTWFYTDDIAVTDAWLRAHPD
                     DVEVIPLCRIPPGQHSHEQHIEELRAALARMLHMHHMMLETTHYRENITDARCIREMH
                     EAPLQALNILSRYPHKKDEV"
     gene            1882..2097
                     /locus_tag="ACHKBL_004656"
     CDS             1882..2097
                     /locus_tag="ACHKBL_004656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001622039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5821402.1"
                     /translation="MWLLIFWLSSPFNVSSGDARQPTLAVHSQAFHTERACRDALRAI
                     KKENVGEFSLRGVCVSDGNTKEEAESS"
     gene            complement(2295..2918)
                     /locus_tag="ACHKBL_004657"
     CDS             complement(2295..2918)
                     /locus_tag="ACHKBL_004657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001466373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO5821403.1"
                     /translation="MQEHIDYQIEKYQFRARNESPRLMRQWADVMRECRDTGAGARER
                     LRIALLNVDYVTSFELPFRLLLTRTPQMIDSVRREWQLSQKKVVFNERQRGCVYSLQN
                     DLSGVPDSFSYNLATRIRRVDSYGMTTRCFTDIAGQVKAPTARLTLALESGLIVTALD
                     GLFWLGIQRIAADVQRLRQSGMTIITRDVEVSDSLTGTTRVVSGYSL"
     gene            3083..>3505
                     /locus_tag="ACHKBL_004658"
     CDS             3083..>3505
                     /locus_tag="ACHKBL_004658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000598960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HFO5821404.1"
                     /translation="MIKYMFSLAIIFSGSLCAAQIQGNVIRVPDGDTLEILQDKKTVR
                     VRLANIDAPEKKQPFGRWSASQLKDLVAGKPVTVQYLQTDRYNRILGRVYTADGTEAN
                     REQIRKGAAWVYPDYNKDYTLPALQRDARALKRGLWADE"
BASE COUNT          942 a          918 c          799 g          846 t
ORIGIN      
        1 tgtcacacag gatattcgac gacgcatcgc ggcctgcttt gttgctatag ataacgatca
       61 tcgtatcatc ggatactata cgttatcagc ggccagtata ttattaactg acttacctga
      121 gcatttgaga aaaaagctac cacgatatcc taccatcccg gctattcgta tgggacgcct
      181 ggcagtaagt ctgtcatcaa aaggaacagg acttggtggg acacttttag ctgatgcgct
      241 atatcgtgca tacacatctg aaattgcagc atatgcgctg attgttgatg caaaagatga
      301 cacggcagtc tcattttaca aacattatgg atttatgacg ctccctgagt ccgggcgaac
      361 tttattcatg ccactggcat cattatctgc aatagaataa ccagaataag tccgggtaaa
      421 aaagggcgca ttgaggtcag ccttcgcaac aatccggcgg cagattccct gccgccgtgt
      481 tgcccgttaa cgtttaactt cgtaggctaa cgttgccaca agcactgtgt ttccctgctc
      541 gacacggaat ccgcaaagcg aatccctcac catccagaca aaacacagta tcgtcacaca
      601 gacgacaatt aacattgtta aaaacgttcg ttgtggcatc gcagcccctc cattgccttt
      661 cggccgtcat cgggctacta taatgttgtc gatgcattac agtagagccc tgggttgatt
      721 gataaaaaaa tcgcctgggg ctttctgctg catgccatcc acaaaacgct ttcggcatac
      781 agcctcaagc gcccgcagct attctaattg ttttcctgtc cgccacaatc cttttcatgc
      841 cgttatattt tttgtatccg gaacaggaaa atactcggga attatcccat ccgaacgcct
      901 caggttttcc gtctacggga ttcgtgttat taccccaaaa tgcaaaatgc ctgaaataag
      961 gagtagtcat gaaagacgaa atacaaagta tgtgcctcac cccggatatc gttaaacagt
     1021 tcaatactct tttgaataca cactgccagt cctcagaaga ggcgctcggc atgctcagcg
     1081 cactgtgttt tgagctcatg atgaaatgtg gtgttcagtc cataaacagc ctcatggtca
     1141 acggtatggg catatccatg caactccagg ggattgatat tgccgaaagg ccaggcaaga
     1201 atattcacta acctgaacgg agcctgaaaa cgctgatttt tcaggcccca gcaggctaaa
     1261 actgatgatg caatgacaac aaaatttctg cgtttgtacc ccacagatga aaaagataaa
     1321 aactcacacc ggacttctcg ccagtaaaga taaaaactgg cgcgtcagcc tctacgaaac
     1381 gccaactgca tggtgtatca gggggcagga gtgctacagc aaaagcaccg gacgccgctg
     1441 tggcagccac gacagtctga gccgactgct cctggacagc attaagcccg ttgaatgagt
     1501 ggtggcacga tgaaagataa caacaccacc gccacccctg aagcgtggaa aatgcgtttt
     1561 ctggccaacg ggacatccgg tacatggttt tacactgacg acattgctgt cactgacgcg
     1621 tggcttcgcg cacatccgga tgatgtggag gttatccctc tctgccggat accgcccggg
     1681 cagcattccc atgaacaaca tattgaagaa ctccgcgcag ccctggcccg gatgctccat
     1741 atgcatcata tgatgctgga aaccacacat taccgggaaa atatcaccga tgcccgttgt
     1801 atccgtgaaa tgcatgaagc gccattacag gcgctcaata ttctgtccag atacccgcat
     1861 aaaaaggacg aggtgtgacg catgtggcta ctgattttct ggctttcatc gccctttaat
     1921 gtctcttccg gtgatgcccg tcagcccaca ctggcggtgc acagccaggc ctttcatacc
     1981 gaacgggcct gccgcgatgc attgcgcgcc attaaaaagg agaatgttgg cgaattttct
     2041 ctgcggggcg tttgtgtttc tgacggaaat acgaaggagg aggctgagag ctcatgacgg
     2101 atattactga cagacataca cttcgccgta tgtcctggtc ggaactcatc acggcaggac
     2161 aggaagcaga atttcagcgt gattacgagc gtaccagaat agtgtggtca ttcgccttac
     2221 atgttgcaac aaccacgatt aacaaaaatc tggccatagc tcatattcga cgctgcgata
     2281 ctttgttgca taaatcaaag gctgtacccg gaaacaaccc gtgtggttcc ggtcagcgag
     2341 tctgagacct caacatcccg ggtgatgatt gtcattccgg actgacgcaa ccgctgcaca
     2401 tctgcggcaa tacgctgaat accaagccag aacagaccat ccagcgcggt gacaattaac
     2461 ccgctttcca gcgccagtgt cagccttgct gtcggggctt taacctgccc ggctatatca
     2521 gtgaaacaac gcgtcgtcat gccatagctg tccacccgcc gtattcgcgt cgcaagattg
     2581 taactgaagc tatccggcac gccggataag tcattctgca gactgtatac acatccccgc
     2641 tgccgctcat tgaatacgac ttttttctgg ctgagctgcc attcccgccg cacgctgtca
     2701 atcatctgcg gtgtccgggt taacagcaga cgaaacggca gctcaaaact ggtcacataa
     2761 tcaacattaa gtaaggcaat ccgcagccgc tccctggctc ctgctccggt atcacggcat
     2821 tcacgcatga catctgccca ctggcgcatc agtcgcggag actcattcct ggccctgaac
     2881 tgatatttct caatctgata atcaatatgt tcctgcatac tgtcaccgga tatctgttac
     2941 tatatcatca tgaatattag cctactggaa ttcatgcgct gcgtgaagca ttacattacc
     3001 ggaaacataa actcaactta acgatattat tccgtaatat aaacgccgcc tgtttttcat
     3061 gaataaaaaa ggatgtaata aaatgataaa atatatgttc tctctcgcta ttattttttc
     3121 aggttccctc tgcgctgcac agattcaggg aaacgttatc cgcgttcctg atggtgatac
     3181 actggaaata ttacaggata aaaaaactgt cagggtccgc ctggcaaata ttgatgcacc
     3241 ggaaaagaaa cagccctttg gacgctggtc tgcctctcag ttaaaagacc tggtggccgg
     3301 taagcctgta accgtacaat atttacagac cgacagatat aaccgtattc tcggcagagt
     3361 ctataccgct gacggcactg aagcgaatcg tgagcaaatc aggaagggag cggcctgggt
     3421 atacccggac tacaacaagg actatactct cccggctctc cagcgcgatg cccgtgccct
     3481 taaacgggga ttatgggcag atgaa
//
LOCUS       DAZFLD010000317         3475 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.319, whole genome shotgun sequence.
ACCESSION   DAZFLD010000317 DAZFLD010000000
VERSION     DAZFLD010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3475
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.319"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(481..552)
                     /locus_tag="ACHKBL_004659"
                     /pseudo
     CDS             complement(481..552)
                     /locus_tag="ACHKBL_004659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001694628.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA distortion polypeptide 3"
     gene            complement(903..1097)
                     /locus_tag="ACHKBL_004660"
     CDS             complement(903..1097)
                     /locus_tag="ACHKBL_004660"
                     /inference="COORDINATES: protein motif:HMM:NF033650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HFO5821405.1"
                     /translation="MNQDKAIYLENKYFSPANEAVRLEREGDYIGAWKKWHDALHYAK
                     GENIDWCIARAEYCKKTGRF"
     gene            complement(1671..2687)
                     /locus_tag="ACHKBL_004661"
     CDS             complement(1671..2687)
                     /locus_tag="ACHKBL_004661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020240588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HFO5821406.1"
                     /translation="MKNPDSPVLSLRDYSTRDTKWDSDRVMADRVAQIYESDSMFSSR
                     GERMFDCSRRLLFAPKVSRLTGEMKLALRKGEFCHVPFCPVCSRRRSLRWMRRLWEAL
                     PKLLVERPAARWLFMTLTVKNPPVENTRETLIRMNAAWKRLSDRKEFRPVLGWLRTTE
                     ITYGRVLGCCHPHFHVLMMVPPSMLSGNGYVKHARWVEIWSECLRVDYEAGVDIRVVK
                     PKQGWKQPDGVTLPDMHRAALESGVIETMKYTVKSSEVVRDPAWFLELARQTYGLRMV
                     ATGGRLKEVLKVDKPETDEDLVGADIPAEPDEFEEQAFWLAFDWGRTEKRYKRNPEAD
                     RMKD"
BASE COUNT          903 a          931 c          738 g          903 t
ORIGIN      
        1 gcctctcatg acgaactgcg gcaaatttct gacgttgcaa actgcaacac ataaaacaca
       61 cagaaaaact cacaattgcc atgtcctcat tgacaccccc ttgccatata gggggatttt
      121 ttcgcctgtc ctgattgtct tttgctgatg gttacggcag attggaaaag agcgcatttg
      181 tggtgctctc tccaaaataa tatttctcat tactcaaact atgatggctt ccccctgaaa
      241 ttcaggggga cttttttaat ttatcgctat gcttcgtaat tcattcagaa tacagacagt
      301 atggttaatc agtttttcat catgaacggt gtgttcagca ataagatgat taaactcaat
      361 aatcaggctg taactctctc tgtatacatc atctctgtta aattcgttag ctctgtattt
      421 tgaataaata ataatgcctt tgtcctgtat actctgaatt tgtttaatca catcaatact
      481 cataaaaaca tccaacaaga accgactgta ggtcaccggg caaacgttgc ggaatggcgt
      541 cagagacgtc attttgcggc gtttgcccta tcctgcatca gaacgctaaa tacaggaatc
      601 tctttatgac gaatccttca tttcaggatt ttttatgtag cgtctactac atgcgatccg
      661 ttggtgaacc gttagttagc ctttagatgg actttagatg gacactagtt agatatttac
      721 tgatttagat ggaccttaaa tggatcttag atggatatta gatggatctt agttagacat
      781 tatgtagata ggaaatatta tggggcgatc ctggaggcac gtgaccgact gtatacaaaa
      841 tgctggcgca ttttgatacg tcgacgtgcc aaaggggacg cccctttgaa accccgccag
      901 aattaaaacc ggcctgtctt tttacaatat tcagctctgg ctatacacca gtctatattt
      961 tcgcctttgg cataatgaag cgcatcatgc cattttttcc atgctccaat gtaatcgcct
     1021 tcacgctcca gtctgacagc ttcgtttgcg ggtgaaaaat atttattttc taaataaatt
     1081 gctttgtcct gattcatact cgtaatttat atttttattt gaattaaaaa agagcggcct
     1141 gtcgggttaa tacatattgg catgactgtt gatatatcag cccaactgca cgctcgtaat
     1201 cgttttaacg tgattttcgc aacatggcaa tccgggttca tctgtccggt aatcaataaa
     1261 aaccgtgcga aaaacgggac aggatgtggg aatgtcggac cgcgcacgcg cactcagaca
     1321 ttccccacct ggtcagggct gcgccccgac accctcgaca ccgttcccgc aatacgatag
     1381 aacatcaggg cgcgtttccg ggtaaacgca tgttaatgac cgggaattca ttgattaggg
     1441 accggagacg cgctacaaaa ctcctgcgcg tttctggcat caactgacca tggggatggt
     1501 ttggcagtag cagcaagcgc cagaaacgcg ctcaaaacgt ctctgacggc ctgaatccaa
     1561 caaaacatac aaaaccattg cccgatgcaa aaaagtgcgt tagaaagcgt ctgagcgcgt
     1621 ttctggcgtc ttttttgttg ctgtgtcgct actcgttgtc agattcgcgc tcaatccttc
     1681 atcctgtcag cttcaggatt ccgcttgtaa cgtttttccg tacgtcccca gtcaaacgcc
     1741 agccagaagg cttgctcctc gaactcgtcc ggttctgccg gaatatctgc cccaaccaaa
     1801 tcctcgtctg tctccggttt atcgactttc agaacctctt tgagacgacc gccggttgcc
     1861 accatccgaa gcccgtaggt ctgccgcgcc agctccagaa accacgccgg atcacgcaca
     1921 acttccgaag atttcaccgt gtatttcatc gtctcaatga cgcctgactc cagcgccgca
     1981 cgatgcatat caggcagggt tacaccatcc ggctgtttcc agccctgttt cggttttacc
     2041 acccgaatat ccactcccgc ctcataatcg acacgaaggc attcactcca gatctccacc
     2101 cagcgagcat gtttcacata accattaccg gacaacatcg aaggggggac catcatcaga
     2161 acgtgaaaat gcggatggca acatcctagc actcgcccgt aagtaatttc cgtcgtacgt
     2221 aaccagccaa gaaccggcct gaattcctta cgatcactca agcgtttcca tgctgcattc
     2281 atccgaatga gtgtttcgcg ggtattttcc acagggggat ttttgacagt cagcgtcatg
     2341 aacaaccagc gggccgcagg acgttcaacc aacagctttg gcaaggcctc ccagagacga
     2401 cgcatccaac ggagagagcg acgacgacta cacaccggac aaaacggaac atgacaaaac
     2461 tcccccttcc gaagagccag cttcatttcg ccagtcaggc gggacacctt cggcgcaaac
     2521 aacaaccgac gggaacaatc aaacatccgc tctccacgag aggaaaacat cgaatcgctt
     2581 tcgtaaatct gcgccaccct gtcagccatc acgcgatcgc tgtcccattt tgtatcccgt
     2641 gtgctgtaat cgcgcaacga gagtaccgga ctatctggat ttttcatttg caccccccaa
     2701 gaactgggca gatgcggaaa ttttgtgtta gcatttttct gtcacctgtg actctgttgc
     2761 cgaacaacga attccaaact ggtgatcgtc ccctggagtg tgaccgcact cgcaggggaa
     2821 ccccctcaaa actgttcatt ttttaaacaa acatttttat ctcttttcca cccattttta
     2881 ccgctcaaag ccgcgccacc actggcctaa gccaaaataa cacagaccgc agttgttgcc
     2941 tatagtatat atacaaggat cgcgcgcacg atcgcccacc aaaaatgcgc gttttttgag
     3001 cgcttccgag cacaaaaaaa caacacatcc aaaccacctg atcatcaggt ttctcaccga
     3061 ctttctgaac aacaaaatca gctttgcagg tggttggtcc cactgcgtga caccaaccac
     3121 catcaccagt gtttttttct cgtaccgtcg tcaatcacaa aatcggaacc cacgccggag
     3181 gcgcgggacc ccacgatttt ctgtttgacg agtcagaaaa cggcgaacgc tgacagattt
     3241 caatctggct gcgtgagacg ttggcggggt gtcggggcgc agccctgacc aggtggtaat
     3301 tgcatagccg tgcgtgcgcg gttatacgat tacacatcct gtcccggaat caggattgtt
     3361 tttcgcgcac aactgcggca aattcacccc gttgcggttg caacgatggg aatatcaaaa
     3421 ctacgaaaat tattcaggca tttgctaccc ctctggtcct ggcctccacc agttc
//
LOCUS       DAZFLD010000318         3434 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.320, whole genome shotgun sequence.
ACCESSION   DAZFLD010000318 DAZFLD010000000
VERSION     DAZFLD010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3434
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.320"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..445)
                     /locus_tag="ACHKBL_004662"
     CDS             complement(<1..445)
                     /locus_tag="ACHKBL_004662"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HFO5821407.1"
                     /translation="MNVIDSGIRDVKFGENVKVVMPVNLYECELGDDVFIGPFVEIQK
                     GCIIGNRSRIQSHSFICENVILGDGCFIGHGVTFANDLFKCGGPDASAESWIRIVLGN
                     TVTVGSGATILTTKICSGAVIGAGSVVTKPVLVKGVYAGNPARLLR"
     gene            complement(959..1690)
                     /locus_tag="ACHKBL_004663"
     CDS             complement(959..1690)
                     /locus_tag="ACHKBL_004663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001075464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
                     /protein_id="HFO5821408.1"
                     /translation="MNQSIHNAYWLSRFDSLLDSAPAQHRAVSLIRVDLRFPEYMPVT
                     IMDPDPDSAVISRFFASLKAKIQAYQRKKRCANQRVHATTLRYFWCREFGKEKGRKHY
                     HVILLLNKDIWCSLGDFSEPSSLATMIQQAWCSALHPEPWQGDGLVHFSRWTPSRKPA
                     AADASPSSDDTPLHSGCSDTRTDSDKRPGGSAVLWAKRGDVEAMQKALERARYLVKNE
                     TKLHDGSGQRNYGCSRGVRCLLDGR"
     gene            2361..2891
                     /locus_tag="ACHKBL_004664"
     CDS             2361..2891
                     /locus_tag="ACHKBL_004664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFO5821409.1"
                     /translation="MKLTEESLNLVIDILNDLICEGKMLNDVERRSLLNAAMTITAVK
                     ERSVVSVSSRKEQSKKEKKEREIDPRFPNAGVAWNEADESLLRDVLEDVPDDEIGKHL
                     FWLAEKLGRTPYSVACKVRQIKKLPAEWKDQFRKISDRIRSSGMSVSEYLQRQGLLSP
                     ESGEENNPVSHNNNVA"
BASE COUNT         1039 a          816 c          705 g          874 t
ORIGIN      
        1 ttctcaaaag tctggctgga ttaccggcat acacgccttt taccagcacg ggctttgtaa
       61 ccacacttcc agcaccaatc actgctccac tgcaaatttt tgttgttaaa atagtcgctc
      121 cgctacccac tgttacagtg ttacctaata cgatacgtat ccagctttct gcagaggcat
      181 cgggaccacc acacttaaaa agatcattag caaaagttac gccatggccg atgaaacagc
      241 catcacctaa aatgacgttc tcacagatga aactgtggga ctgaattcgg ctccggttgc
      301 caatgatgca tcccttctgt atttccacaa agggaccaat aaaaacatca tcccccagtt
      361 cacattcata gaggtttact ggcataacga ctttcacatt ttcaccaaat ttcacatcac
      421 gaatgccgct gtcaataacg ttcatatccg ttgtatcctc cctcaggatg attcttatca
      481 gaacatttca cctgtcagaa tattcccggt aatgtgatac aagatccagt ctcaccgggt
      541 accacgtgtt attgcccgta tccttttgcc cgttacgagt ctacaactgg ttttgtattt
      601 tgcaatccag gcctgttaca ccagagcatc ctctcacttc cccctcactg gtcagtccgg
      661 gattcaacat catcctccag cactaaccct ataccagtcc tgaaccttcc ctcgccagca
      721 gcctactgtt caccacaatc ctcttccgtg aatggcagta agtgagctgc accaaaataa
      781 atcggtactt ccgatgacag acagtggaag tgccaggaga aatatgaaca tgagaatgga
      841 agcagataaa aataccgttg catgacttca ggacgggtta tcagaatacg agctaagggc
      901 gtcagccaac gcccttaccg tggtatacac tcaggcaccg gataccattt taccgggttc
      961 acctgccatc cagtagacac ctcaccccac gactgcaacc ataattacgt tgacctgaac
     1021 cgtcatgcag cttcgtttca tttttcacga gataacgggc tctctccagg gctttctgca
     1081 ttgcttccac atcaccacgc ttggcccaga gcacagcgga tccacccggc cttttatcag
     1141 aatccgtccg ggtatcagaa catccactat gcaaaggcgt gtcatcggaa gaaggggaag
     1201 catcagccgc tgctggttta cgggaaggcg tccacctgga aaaatggacc aggccatcgc
     1261 cttgccaggg ctcaggatgc agagcgctgc accatgcctg ttggatcatc gtcgccagcg
     1321 aagacggttc gctgaaatcc ccgagcgagc accagatatc tttgttgagc agtaatatca
     1381 cgtgataatg tttcctgcct ttttccttgc caaactcccg acaccagaaa taacgcaggg
     1441 tggttgcatg cacacgctga ttggcacatc tttttttccg ctggtaagcc tgaattttgg
     1501 ctttcaggga tgcaaaaaaa cgagaaatca ccgctgaatc cggatcggga tccatgatgg
     1561 tgacaggcat atactcaggg aaacgtaaat ccacccggat taacgagacg gcacggtgct
     1621 gcgccggggc actgtcgaga aggctgtcaa aacgggataa ccagtaagca ttgtgtattg
     1681 attgattcat gatgagagat ctccgttgat ttggattgac ttcacatcat tcatcaacgc
     1741 ggtgaaactt tacggcatcc cgccaatgag caacgccaat agctcctgat taaaacggaa
     1801 tgaatcgtcc cgtaacgaaa agataatcct gcacacgaca gcaaaacgta aaacaacagc
     1861 atgaatgagc taaatgttta aagaaaaaat gtcttttcac attatttttc agcaagtaat
     1921 cataccagca tccctaacat acgaaatcac atagtgatcg atcgctgatt cgcttaacaa
     1981 ctgatcgaat acaccggaat ttacactaac atcctgatgg gtaacggaag actaaaaaac
     2041 ctgtgttcta ttgattgcac ggaatgcctg tgcactggcc tgaccagtaa gcacaattct
     2101 acgagtagta agatatctac gccagaacca tgcggcagta gccaccatat gttgccaggg
     2161 tgaaaaataa gctcagagct ataggggagt aattcccgtt aatgtcatcg ctgtacctca
     2221 atcagttcac aaactggctg aaacgttaag ctgaagcctt tattcttcag atgtattttc
     2281 cggtttacct gtaaattatc ttctgatatt tatcacatta gtagttgtgc tgattatcta
     2341 catacttcaa ggtctgaaat atgaagctaa cagaagaaag tctgaatcta gtcattgaca
     2401 tcctgaatga tttgatctgt gaaggaaaaa tgctgaacga tgtggagcgc agatctctgc
     2461 tcaatgcagc tatgacaatt acagcggtaa aagaacgttc tgtcgtatct gtatcatccc
     2521 gaaaagagca aagtaagaag gagaagaagg aaagagagat cgatcctcgt ttcccaaatg
     2581 ctggtgtggc atggaatgag gcagacgaga gtttattgcg agatgttctg gaggatgtac
     2641 cggacgatga aattggtaaa caccttttct ggctggcaga gaaactaggg cgaaccccgt
     2701 acagtgtagc ctgtaaagtc agacaaatta agaaattacc tgcagaatgg aaggatcaat
     2761 ttcgtaaaat ctctgacagg atccgtagtt ctggaatgtc agtcagtgaa tatctacaac
     2821 gccagggact gttaagccca gagtcagggg aagaaaacaa tcctgtcagt cataacaaca
     2881 atgtagctta atcctgaaga agatgaacag tttatactct ttaaactgtt cccttaattt
     2941 tagaaaaaca gtttgtgtaa gtgcatgctc caacattctg aaggagttat ttcttacaat
     3001 acatcataat cattggcaga tcctcatctg aatcaaaaca ccacaaccaa tcatcacact
     3061 ttttctgaaa agagccttat gatgaagata atcaccttac gctgagaaaa aacaccaggg
     3121 ccagttaatc accttatcaa tctcgccgtt acaaaatcat ccataagcca tttaacagtg
     3181 ttatcgcaaa ctcacagtaa atcgcatcag gttacctcaa tcggatttga gttaattcac
     3241 tgggctttac gtaaagatac ttttctcagg ttaacgacta aaacacgaaa actcagaaaa
     3301 ggttaaacac aggttcagta aaatcgaata tcatcttgat tgcttaaaca ggacagaggg
     3361 ttttatagtg aggttaactg attttagcga tcgtaaaaaa aggactaaca aatttactaa
     3421 actggatgtg tatc
//
LOCUS       DAZFLD010000319         3405 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.321, whole genome shotgun sequence.
ACCESSION   DAZFLD010000319 DAZFLD010000000
VERSION     DAZFLD010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3405
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.321"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..589)
                     /gene="dsbA"
                     /locus_tag="ACHKBL_004665"
     CDS             complement(<1..589)
                     /gene="dsbA"
                     /locus_tag="ACHKBL_004665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA"
                     /protein_id="HFO5821410.1"
                     /translation="MKKIWLALAGLVLAFSASAAQYEDGKQYTTLEKPVAGAPQVLEF
                     FSFFCPHCYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKDLTQAWAVAMALG
                     VEDKVTVPLFEGVQKTQTIRSASDIRDVFINAGIKGEEYDAAWNSFVVKSLVAQQEKA
                     AADVQLRGVPAMFVNGKYQLNPQGMDTSNMDVFVQQ"
     gene            complement(606..1592)
                     /gene="srkA"
                     /locus_tag="ACHKBL_004666"
     CDS             complement(606..1592)
                     /gene="srkA"
                     /locus_tag="ACHKBL_004666"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response kinase SrkA"
                     /protein_id="HFO5821411.1"
                     /translation="MNNSAFTFQTLHPDTIMDALFEQGIRVDSGLTPLNSYENRVYQF
                     QDEDRRRFVVKFYRPERWTPDQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFY
                     FAVFPSVGGRQFEADNIDQMEAVGRYLGRMHQTGRKQLFIHRPTIGLNEYLIEPRKLF
                     EDATLIPSGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHAGNILWRDGPMFVDL
                     DDARNGPAIQDLWMLLNGDKAEQRMQLETIIEAYEEFSEFDTAEIGLIEPLRAMRLVY
                     YLAWLMRRWADPAFPKNFPWLTGEDYWLRQTATFIEQAKVLQEPPLQLTPMY"
     gene            complement(1669..1938)
                     /locus_tag="ACHKBL_004667"
     CDS             complement(1669..1938)
                     /locus_tag="ACHKBL_004667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HFO5821412.1"
                     /translation="MKCKRLNEVIELLQPAWQKEPDLNLLQFLQKLAKESGFDGELAD
                     LTDDILIYHLKMRDSAKDAVIPGLQKDYEEDFKTALLRARGVIKE"
     gene            2008..2592
                     /gene="mobA"
                     /locus_tag="ACHKBL_004668"
     CDS             2008..2592
                     /gene="mobA"
                     /locus_tag="ACHKBL_004668"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HFO5821413.1"
                     /translation="MNLMTTITGVVLAGGKARRMGGVDKGLLELNGKPLWQHVADALM
                     TQLSHVVVNANRHQEIYQASGLKVIEDSLADYPGPLAGMLSVMQQEAGEWFLFCPCDT
                     PYIPHDLAARLNHQRKDAPVVWIHDGERDHPTIALVNRAIEPLLLEYLQAGERRVMAF
                     MRLAGGHAVDFSDRKEAFINVNTPEELARWQEKR"
     gene            2574..3101
                     /gene="mobB"
                     /locus_tag="ACHKBL_004669"
     CDS             2574..3101
                     /gene="mobB"
                     /locus_tag="ACHKBL_004669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobB"
                     /protein_id="HFO5821414.1"
                     /translation="MAGKTMIPLLAFAAWSGTGKTTLLKKLIPVLCARGIRPGLIKHT
                     HHDMDVDKPGKDSYELRKAGAAQTIVASQQRWALMTETPDEEELDLHFLASRMDTSKL
                     DLILVEGFKHEEIAKIVLFRDGAGHRPEELVIDRHVIAVASDVPLNLDVALMDINDVE
                     GLADFVVEWMQKQDG"
     gene            complement(3204..3319)
                     /gene="rrf"
                     /locus_tag="ACHKBL_004670"
     rRNA            complement(3204..3319)
                     /gene="rrf"
                     /locus_tag="ACHKBL_004670"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT          885 a          840 c          823 g          857 t
ORIGIN      
        1 actgctgaac aaaaacatcc atattgctgg tatccatacc ctgcggattc agctgatatt
       61 taccgttaac aaacatcgcc ggaacgccac gcaattgcac gtcagctgca gctttttcct
      121 gctgagcgac cagggatttc accacgaagc tgttccacgc tgcgtcgtac tcttcacctt
      181 taatacctgc gttgataaat acatcgcgga tatcagatgc tgaacgaatg gtctgggttt
      241 tctgtacgcc ttcaaacagc ggaacagtga ctttgtcttc cacgcccagt gccatcgcca
      301 ctgcccatgc ctgagtcaga tctttgccca ggtcaccccc catgaagttg acgtggtatt
      361 tagtcatctt cacgccttcc ggcagttttt tcttcacgtt atcagaaata tgcagaactt
      421 cttcaaactg atagcagtgc gggcagaaga aagagaaaaa ctccagcact tgcggcgcgc
      481 cagcaactgg tttttccagg gtagtgtact gtttaccatc ttcatactgc gccgccgatg
      541 cgctaaacgc taaaactaaa ccagccagcg ccagccaaat ctttttcatg atctactctc
      601 tccgattaat acataggtgt taattgcaaa gggggttctt gtagaacttt tgcctgttct
      661 ataaaagtcg ccgtctgtcg cagccagtaa tcttccccgg ttaaccacgg gaaatttttc
      721 gggaacgcgg gatcagccca acgccgcatt agccaggcaa gataataaac caaacgcatg
      781 gcgcgtaaag gttcaatcag tccgatttca gcggtgtcga actcgctaaa ttcttcataa
      841 gcttcaataa tagtttccag ttgcatccgc tgctcggctt tatcgccatt gagcaacatc
      901 cacaaatcct gaatagccgg accattacgt gcatcatcca gatcaacaaa cattggacca
      961 tcgcgccaga gaatattccc ggcgtggcag tctccatgta gccgcagaac ggtgaaatct
     1021 tcccgccagt gtgctgtaac ggcggcgatc agctcatctg tcgctttcag aaatgccgct
     1081 ttcaacccgg aaggtatcag tgtagcgtcc tcaaacagct tgcgaggctc aatgagatat
     1141 tcattcagac cgatggtcgg gcgatggata aaaagctgtt tgcgccccgt ctggtgcata
     1201 cgccctaaat aacgcccaac cgcttccatc tgatcgatat tatcagcttc gaactggcga
     1261 ccaccgacgc ttggaaaaac agcgaaataa aatccctgat gattcaataa agtctgaccg
     1321 ttaaaggcca caggtgctgc gaccggaact tcatcattta ccaactgcaa cgcaaattga
     1381 tgttcttcga ggatttgatc gggtgtccaa cgttcaggac gataaaattt gacgacaaaa
     1441 cgtcgacgat cttcgtcctg aaattgatag acacggtttt catagctgtt aagcggggta
     1501 agaccggaat ccacccggat cccttgctca aacagagcat ccatgatggt atccgggtgt
     1561 agtgtctgga aagtaaaagc gctgttgttc atcccatcat ccggaaaaca ttacgaatga
     1621 tgaaggatat catttcacga tgattttggt ggcaccgctt acaagctttt actctttaat
     1681 tacgccgcga gcgcgtaaca gcgccgtttt gaagtcttct tcataatctt tctgcaaacc
     1741 cgggatcacc gcatctttcg cggaatcacg cattttcagg tgatagatca gaatgtcatc
     1801 cgtcaaatcc gccagttcgc cgtcaaaacc tgactctttc gccagtttct gcaaaaattg
     1861 cagcaggtta aggtctggct ctttttgcca ggctggctgg aggagttcaa taacttcatt
     1921 cagacgttta catttcatag taatgctcct tactcttgag acagacacgt tagcagggtc
     1981 aatcccacaa taaaagaggc gatatcggtg aatctgatga cgacgataac aggcgttgtg
     2041 ctggcaggcg gtaaagccag acgaatgggc ggcgtagata aaggattgct tgaattaaac
     2101 ggcaaaccat tatggcaaca tgtcgctgac gcgcttatga cgcagctctc tcacgtcgtg
     2161 gttaatgcta atcgtcatca ggaaatctat caggcaagcg gtctgaaagt gattgaagat
     2221 tcactggcgg attacccagg ccctctggca ggaatgcttt cagtaatgca gcaggaagcg
     2281 ggtgagtggt ttttgttttg tccgtgcgat acgccttaca ttccccatga tttagccgcc
     2341 cggcttaatc atcagcgcaa agatgcgcct gtcgtgtgga tccacgacgg tgaacgcgat
     2401 cacccgacta ttgctctggt aaaccgcgca attgagcctt tattactgga atatctgcaa
     2461 gcaggagaac gccgggttat ggcatttatg cgtctggctg gcggtcatgc ggttgatttc
     2521 agcgatcgta aagaggcatt tataaacgtg aatacgccag aggagcttgc ccgatggcag
     2581 gaaaaacgat gataccgtta ctcgcctttg ccgcgtggag tggtactgga aaaactacac
     2641 tgctgaaaaa actgatcccg gtattatgcg ccagagggat ccgtccggga ctgattaagc
     2701 atacgcacca tgatatggat gttgataaac cgggcaaaga tagctatgag ttgcgcaagg
     2761 ctggcgcggc acaaaccatc gttgccagcc agcaacgatg ggccttgatg acggaaacac
     2821 cagacgaaga agagctggat ctacattttc tcgcaagtcg aatggatacc tcaaagctgg
     2881 atttgattct ggtcgaaggg tttaagcatg aagagatcgc aaagattgtg ctgtttcgcg
     2941 atggagccgg acatcgaccg gaagaattag tgatagacag acatgttatt gctgtagcca
     3001 gtgatgtgcc gcttaatctt gatgtcgcgt taatggatat taatgatgtt gaggggctgg
     3061 ctgattttgt ggtggagtgg atgcaaaagc aggacggata agcaaaagta ggctggataa
     3121 gacgcgccag cgtcgcatcc ggtatttatt ctgctccagc gcaaaaaggc catcctgacg
     3181 gatagccttc tgcttaattt gatgcctggc agttccctac tctcgcatgg ggagacccca
     3241 cactaccatc ggcgctacgg cgtttcactt ctgagttcgg catggggtca ggtgggacca
     3301 ccgcgctaag gccgccaggc aaattctgtt ttatcaacac gtctcgttat gacatgtcga
     3361 tttaatctgt atcaggctga aaatcttctc tcatccgcca aaaca
//
LOCUS       DAZFLD010000320         3383 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.322, whole genome shotgun sequence.
ACCESSION   DAZFLD010000320 DAZFLD010000000
VERSION     DAZFLD010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3383)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3383)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3383
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.322"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..126
                     /locus_tag="ACHKBL_004671"
     CDS             <1..126
                     /locus_tag="ACHKBL_004671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011815933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFO5821415.1"
                     /translation="LLITFLPVFLLPVLGDIGFANTTFSDADFGALGILLGIIVR"
     gene            complement(342..626)
                     /locus_tag="ACHKBL_004672"
     CDS             complement(342..626)
                     /locus_tag="ACHKBL_004672"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFO5821416.1"
                     /translation="MEQKTLSAEPRRSFSNEFKLQMVKLALQPRGSVARIAREHDIND
                     NLLFKWLRLSLIKGRISRRLPVTNSSGIGVELLPVEMTLDERPYPVFYAR"
     gene            complement(1048..2061)
                     /locus_tag="ACHKBL_004673"
     CDS             complement(1048..2061)
                     /locus_tag="ACHKBL_004673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HFO5821417.1"
                     /translation="MSTRINLWRALFGEKPRILLENSDFTVTSFRYDSGVEGLKIANS
                     RGHLIILPWMGQMIWDAQFDGHGLTMCNMFRQPKPATEVIETYGCFAFHSGLLANGCP
                     SAEDTHLLHGEMACAAMDEAWMELEGDMLRLTGRYEYVKGFGHHYLAQPTVVLHKSST
                     LFDIKMAVTNLASVDMPLQYMCHMNYAYIPNATFSQNIPDEILRLRESVPSHVNPTTQ
                     WLAFNQRIMQGEASLSTLNQPEFYDPEIVFFADKLDAYTDQPEFRMIAPDGTTFVTRF
                     SSAELNYVTRWILYNGEQQVAAFALPATCRPEGYLAAQRNGTLIQVAPQQTRTFTVTT
                     GIE"
     gene            complement(2073..>3383)
                     /gene="fucP"
                     /locus_tag="ACHKBL_004674"
     CDS             complement(2073..>3383)
                     /gene="fucP"
                     /locus_tag="ACHKBL_004674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015150 - fucose transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0015535 - fucose:proton symporter activity
                     [Evidence IEA];
                     GO_process: GO:0015756 - fucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HFO5821418.1"
                     /translation="DKNIIQMPDGYLNKTPLFQFILLSCLFPLWGCAAALNDILITQF
                     KSVFSLSNFASALVQSAFYGGYFLIAIPASLVIKKTSYKVAILIGLTLYIGGCTLFFP
                     ASHMATYTMFLAAIFAIAIGLSFLETAANTYSSMIGPKAYATLRLNISQTFYPIGAAS
                     GILLGKYLVFSEGESLEKQMSGMNAGQIHNFKVLMLENTLEPYKYMIMILVVVMVLFL
                     LTRFPTCKVAQTSHHKRPSAMDTLRYLARNPRFRRGIVAQFLYVGMQVAVWSFTIRLA
                     LELGDINERDASNFMVYSFACFFIGKFIANILMTRFNPEKVLILYSVIGALFLAYVAL
                     APSFSAVYVAVLVSVLFGPCWATIYAGTLDTVDNEHTEMAGAVIVMAIVGAAVVPAIQ
                     GYIADMFHSLQLSFLVSMLCFVYVGVYFWRESKVRTALAEVTAS"
BASE COUNT          982 a          782 c          751 g          868 t
ORIGIN      
        1 ttgcttatta ccttcctgcc agtattctta ttgccggtgc tgggtgatat tggttttgct
       61 aacaccacct tcagtgatgc tgactttggc gcactgggta ttttgttagg gattatcgtt
      121 cgctaatatt ttgatgacat taatatcctc caggatttca ggaacgtgga ggatattaat
      181 tcctcttatt ttctccattt ttctttcttc cagtcaggac tgcatccgat taatcgtaag
      241 cgctccggga agtacgtagc gtaaggatta ttttacagct gagaagtttc agtgcagcag
      301 ttcatgcaca cggttcgacg gctagtcatt gatcttcata attacctcgc gtaaaaaacg
      361 gggtaaggcc gctcatccag cgtcatctcc acaggtaata attcaacgcc aatgccagaa
      421 gagttcgtta ccggaagacg ccgtgatata cgccctttga tcagcgagag cctgagccat
      481 ttgaacagaa gattatcatt gatatcatgc tcccgggcaa tacgggcaac agaacccctt
      541 ggttgtaaag ccagtttaac catttgaagt ttaaactcat ttgaaaatga cctgcggggt
      601 tcagctgata atgttttttg ttccataaca ggtgtccact agttgaaaag gtgggtacct
      661 atgttaccaa tactggctta atggctacat acagcagtcg gtttacgcat acacttttat
      721 agtgcactcc ggcctcatga atataacggt ggattaccgc cgaacgaatc ggaaaataga
      781 tactggaaaa aaactgaaat tgtgaccagt ttttgttgac cactacaaat aaataaaaga
      841 gttgcagtgc ttgctcggta agaaaacaat ggaggttaag ctcctcaaag aagctgtgga
      901 gtacaccact ctacagtagt tgcgcaaatt tggagtggac gataatgtga tcgtctataa
      961 gggcaacgct atcatagtct tgtcctggcg ggtaaaaaaa cgcgcttacc ttaacgataa
     1021 gcgcgccgct gttcaggcct tgagtggtta ttcaattcct gtggtgactg taaaagtgcg
     1081 cgtttgctgc ggtgcaacct gaatcagcgt gccattacgt tgcgcggcaa gatacccctc
     1141 aggccgacag gttgcaggta atgcaaaggc ggctacctgt tgctctccgt tataaaggat
     1201 ccagcgtgtc acataattta gttcggcact ggagaaacga gtaacaaacg tagtgccatc
     1261 gggagcgatc atgcgaaact ctggctgatc tgtgtaagcg tccagtttgt ctgcaaagaa
     1321 gacaatttct ggatcataaa actccggttg attcagcgtc gacagagagg cttctccctg
     1381 cataatccgt tgattaaacg ccagccactg agtggtggga ttaacatgcg aaggtactga
     1441 ttcacgcaac cttaatattt cgtccggtat attctgactg aacgtagcat ttggtatata
     1501 tgcataattc atgtggcaca tatattgtag tggcatatct acagaagcca gattggttac
     1561 ggccatctta atatcgaaca gtgtagagga tttgtgaagg accactgttg gctgagccag
     1621 ataatgatgc ccgaacccct ttacatactc gtaacgcccg gtaaggcgta acatatctcc
     1681 ctctaattcc atccatgctt catccatcgc ggcacaggcc atttcaccgt gtagcagatg
     1741 agtatcttcc gcagatgggc agccattagc cagcaaacct gaatgaaaag caaaacagcc
     1801 ataggtctct atcacctctg tcgccggttt aggctggcga aacatattgc acatggtgag
     1861 gccgtgtcca tcaaattgcg catcccaaat catctgcccc atccagggaa gaataatcaa
     1921 atgtccacga ctgtttgcaa ttttaagccc ctcgacaccg ctgtcatatc gaaaagacgt
     1981 gacagtaaaa tcactatttt ccagcaagat acgaggtttc tcgccaaaaa gcgcccgcca
     2041 caaattaata cgcgtactca taacggttct cctcaggacg ctgtgacttc agccagtgcg
     2101 gtacgtactt tgctttcacg ccagaagtag actccgacat agacaaagca gagcatagaa
     2161 accaggaatg aaagctgtag tgagtggaac atatctgcaa tatatccctg aattgccgga
     2221 accaccgcgg caccgacaat agccataaca atgactgctc ctgccatttc tgtatgttcg
     2281 ttatcaactg tatccagtgt tcctgcatag atcgtcgccc agcaagggcc aaacaaaaca
     2341 cttaccagga cggcgacata gaccgcgctg aaacttggag ccagtgcaac atatgccagg
     2401 aacagcgccc ctataacgga atagagaatc aatacttttt ccggattaaa acgcgtcata
     2461 aggatattgg ctataaactt gccaataaag aagcaggcaa agctatagac catgaagttt
     2521 gaagcatcac gttcgttgat atcgcccaac tccagcgcca gacggatggt aaatgaccat
     2581 actgcgacct gcatacccac ataaaggaac tgcgccacaa taccgcgacg aaagcgcgga
     2641 tttctagcca gatagcgcag cgtatccatt gctgacgggc gtttatggtg acttgtctgt
     2701 gccactttac aggttgggaa gcgggttaaa aggaacaaca ccatgaccac aaccagaatc
     2761 ataatcatat acttatacgg ttcaagggtg ttctctaaca tcagcacctt aaagttgtga
     2821 atttgcccgg cgttcattcc tgacatctgc ttttcaaggc tttccccctc ggagaaaacc
     2881 agatatttgc ccaataaaat accagacgca gcaccaatcg gataaaaggt ctggctgata
     2941 ttgagccgca atgtggcata ggcttttgga ccgatcattg aactgtatgt gttcgctgca
     3001 gtttcaagga aactcaggcc aatcgcaatc gcaaaaatag ctgcaaggaa catagtgtag
     3061 gttgccatat gcgaggcagg gaaaaaaagt gtacaaccac caatatacag cgtcaggcca
     3121 attaaaattg ccaccttata actggtcttt ttaatcacaa gggatgctgg tattgcaatt
     3181 aaaaaataac ctccataaaa tgcgctctgc accaatgctg aagcaaagtt acttagcgaa
     3241 aatacacttt tgaattgagt gattaatatg tcatttaatg cagctgcgca tccccatagc
     3301 gggaataaac acgataacaa aataaactgg aacaagggag tcttattcag atacccatca
     3361 ggcatctgaa tgatgttttt atc
//
LOCUS       DAZFLD010000321         3371 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.323, whole genome shotgun sequence.
ACCESSION   DAZFLD010000321 DAZFLD010000000
VERSION     DAZFLD010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3371
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.323"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            983..1210
                     /locus_tag="ACHKBL_004675"
     CDS             983..1210
                     /locus_tag="ACHKBL_004675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000483811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF465 domain-containing protein"
                     /protein_id="HFO5821419.1"
                     /translation="MFPEYRELISALKGNHKRFDSLFEKHNKLDHEIKRIEDEFGSAY
                     STKVVRLKLEKLEIKREIQKILEDESEKGER"
     gene            1532..2107
                     /gene="dolP"
                     /locus_tag="ACHKBL_004676"
     CDS             1532..2107
                     /gene="dolP"
                     /locus_tag="ACHKBL_004676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001238641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006970 - response to osmotic stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/outer membrane stress-associated
                     lipid-binding lipoprotein"
                     /protein_id="HFO5821420.1"
                     /translation="MRLLYSAVVLTSALLLQGCVAAAVVGTAAVGTKAATDPRSVGAQ
                     VDDEVLELRVSSALSKDEQIRKEARISEIAYQGRVLLIGQSPGRELSARARQIALGVD
                     GVQEVYNEIRQGTPVSPVIMAGDAWLTTKIRSQLLTSARVKSSSVKVVTENSEVFLLG
                     LVTEQEGRAAADVASRVSGVKRVTTAFSYIR"
     gene            2525..2806
                     /locus_tag="ACHKBL_004677"
     CDS             2525..2806
                     /locus_tag="ACHKBL_004677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HFO5821421.1"
                     /translation="MPSATQTARLEARISTELHAMLKRAAEIQGRTMTDFIIAAVQDA
                     AQRAIEQTDIIRLSRTDQEIFAQALLSPTEPNDALKRAFKRRNNMLYTK"
     gene            2812..3300
                     /locus_tag="ACHKBL_004678"
     CDS             2812..3300
                     /locus_tag="ACHKBL_004678"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO5821422.1"
                     /translation="MFQLTPFNSTYDRTSFLCGSDALDRYIKEHVTQDIRRRIAACFV
                     AVDNDHHIIGYYTLSAASILLTDLPEHLRKKLPRYPTIPAIRMGRLAVSLSSKGTGLG
                     GALLADALYRAYTSEIAAYALIVDAKDDTAVAFYKHYGFMAFPESGRTLFMPLASLST
                     IK"
BASE COUNT          940 a          698 c          787 g          946 t
ORIGIN      
        1 atgatatctg ttttatgtat acgatgtagc cagtgattta gtacattgct ggctatatca
       61 cttttcccat tttgtgataa gtctgctttg aaacgatggt gtccctcctc ctgtattgtg
      121 aaagttgcca catttttttc ttcatatccc tgaacatccc cgtcgtgcct tatataattg
      181 actccattaa gaagttgtca gaataattat ttctgattgc aacggagtta atttcagtga
      241 tggtaaagat atccgtggca ccataacgca tttttcatca tcagtatagt ggtatgcgtg
      301 aacatatatg gtggcatttt gcctgatgtt gttaaggctt ttcagcaggt ctacagtcaa
      361 ctccatcgag gtatccttca ggttgttatc attcgttgat gatgcgatct gttgaaggcg
      421 ttggcgacaa ttaattcttt ttctcttctg aaaaatttac ggaatacaaa aaagatctgt
      481 ttttaactga ttgttacttt ctgaagatat tctctgctcc atttgctttc cgggtaagac
      541 gtatggctgg ttatggtatg gtcggactgc agccacaggg ggatgactgt aatacctctg
      601 atcaccacag tcagtcatct gtaaatgtta aatacctcac agttcagtca gtggattatt
      661 gtcggtctta cgggatattt gttaaaaaaa tactgcatgg tgaatccccc tgagcggcgg
      721 ggcatatcag tatcgtaggt gtttggtcac atcccttatc tgtgcgggtt caggtgctga
      781 tactgaactc accgggaggc acccggcacc atgctacata acactccatt acgctacact
      841 cgcccctctc cggaggggtt ttctctgcag gatgtgaata tgagatgctg catcattcac
      901 tgcgaatatt gatggtgaga atctttaata atattaatgt ttatatgtat tgttacagtg
      961 ttgatgaaag gagataaaaa caatgttccc ggagtacaga gaactgattt cagcattgaa
     1021 aggaaatcat aaacgctttg attctttatt tgaaaagcat aataaacttg atcacgaaat
     1081 aaaaagaatt gaagatgaat ttggttcagc gtattcgacg aaagtggtca ggctgaagct
     1141 tgaaaaactt gagatcaaaa gagaaatcca gaaaatcctt gaagatgagt cagaaaaggg
     1201 cgaacggtaa tcataaatgg tatattcaaa gacgtgtttt ctctgtttta tgaaaaaggc
     1261 accgggggct ccacggtgcc ttttagcctg tgtataacga aaaggagatc ctgttcagcg
     1321 aaccagatag ttaaaaaaag aatctttacg acggtatcaa agttttcgtg caaaaaacag
     1381 atacagatat gccacgtaac gcatccgcta tttccggctg tgccggagga taataacagt
     1441 ctgtagcttg ttttgtttca ctgaaggtaa actttttgta cgataaattc cgggaatatg
     1501 aagtaaaaat caaaacaatt ccgggggaga tatgcgttta ttatattctg ctgttgtcct
     1561 tacttcagcc ttattgttac agggatgcgt ggctgccgct gtggtgggta cagctgctgt
     1621 cgggacaaag gcagcaaccg acccacgcag tgtcggagcg caggtggatg atgaagtgct
     1681 ggagctgagg gtcagcagtg cactttcaaa agatgaacaa atcagaaaag aagcacgcat
     1741 cagtgagatt gcatatcagg gacgcgtgct gctgatcggt cagtctccgg gccgtgaatt
     1801 atctgcccgt gcccgtcaga tagcactcgg tgtcgatggt gttcaggagg tctataatga
     1861 aatccggcag gggacacccg tcagtccggt cataatggca ggtgatgcct ggcttacgac
     1921 aaaaattcgt tctcagttac tcacaagtgc ccgtgtcaaa tcatccagtg tcaaggtggt
     1981 gacagaaaac agcgaagtct ttttactggg cctggttaca gagcaggaag ggcgagcggc
     2041 agcagatgtt gccagtaggg tgagcggagt gaagcgggtc acgacggcct tctcctatat
     2101 ccggtgaagg cagtatcaga gttgccttta gtgtaaattt acgttaaaaa tcagaagagc
     2161 acgtttccgg gccgttcatg ccaccacgtg aacattcgtc gcggaatacc ggaggcgtgc
     2221 tcattataat aaccaatata cataattcct ctttgacacc cccctgaatt cgggggattt
     2281 ttttgcccgg gtatgttcac tcacagataa aatcccggtg tactccggat gtcactgctg
     2341 gtaaataatc tgagctttta tctgtatgca atggtcaaag atcgtggcgt taacatcccc
     2401 cggatagttt tgtctgaatc tgatttttct tttcaggcag aatgtctgtc tgccgtcccg
     2461 gtgtcagagt gtaatttgca caatacggca atttgccgta cagtgtattc aggaggtaca
     2521 aatcatgcca tctgcaacac aaactgcccg actggaagca aggatcagca cagaactaca
     2581 tgccatgctg aagcgcgctg cagaaatcca ggggcgcact atgaccgatt ttatcattgc
     2641 tgccgttcag gatgcggccc aacgcgctat cgaacaaacc gatataatcc gactgtcccg
     2701 gacagatcag gaaatttttg ctcaggcatt actgtcacca acagagccga atgatgcgct
     2761 taaacgggcc tttaaacgcc gtaataatat gctgtacaca aaataaatat aatgtttcaa
     2821 ctgacaccat ttaacagcac atatgacaga acctcatttt tatgtggctc tgatgctctg
     2881 gaccgttaca taaaagagca tgtcacacag gatattcgac gacgcatcgc ggcctgtttt
     2941 gtcgctgtag ataacgatca tcatatcatc ggatactata cgttatcagc ggccagtata
     3001 ttattaactg acttacctga gcatttgaga aaaaagcttc cgcgatatcc cactattccg
     3061 gctattcgta tgggacgact ggcagtaagt ctgtcatcaa aaggaacggg gcttggggga
     3121 gcacttttag ctgatgcgct atatcgtgca tacacatctg aaattgcagc atatgcgctt
     3181 attgttgatg caaaagatga cacggcagtc gcattttaca aacattatgg ctttatggcg
     3241 ttcccggagt ccggaagaac tttattcatg ccattggcat cattatccac aataaaataa
     3301 acagaataaa tcagggaaat acggtgcttg tggcaacgtc agcccacgaa gttaaatgtt
     3361 aacgggcaac a
//
LOCUS       DAZFLD010000322         3362 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.324, whole genome shotgun sequence.
ACCESSION   DAZFLD010000322 DAZFLD010000000
VERSION     DAZFLD010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3362
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.324"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..108)
                     /locus_tag="ACHKBL_004679"
     CDS             complement(<1..108)
                     /locus_tag="ACHKBL_004679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015953002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5821423.1"
                     /translation="MTSREARLGMFLASLLRRIAFSYYDYKAYNFNIEKT"
     gene            396..1712
                     /gene="shiA"
                     /locus_tag="ACHKBL_004680"
     CDS             396..1712
                     /gene="shiA"
                     /locus_tag="ACHKBL_004680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate transporter"
                     /protein_id="HFO5821424.1"
                     /translation="MDSTLISTRPDEGTLSLSRARRAALGSFAGAVVDWYDFLLYGIT
                     AALVFNREFFPQVSPAMGTLAAFATFGVGFLFRPLGGVIFGHFGDRLGRKRMLMLTVW
                     MMGIATALIGILPSFSTIGWWAPILLVTLRAIQGFAVGGEWGGAALLSVESAPKNKKA
                     FYSSGVQVGYGVGLLLSTGLVSLISMMTTDEQFLSWGWRIPFLFSIVLVLGALWVRNG
                     MEESAEFEQQQHNQAAAKKRIPVIEALLRHPGAFLKIIALRLCELLTMYIVTAFALNY
                     STQNMGLPRELFLNIGLLVGGLSCLTIPCFAWLADRFGRRRVYITGALIGTLSAFPFF
                     MALEAQSIFWIVFFSIMLANIAHDMVVCVQQPMFTEMFGASYRYSGAGVGYQVASVVG
                     GGFTPFIAAALITYFAGNWHSVAIYLLAGCLISAMTALLMKDNQRA"
     gene            1814..3268
                     /gene="amn"
                     /locus_tag="ACHKBL_004681"
     CDS             1814..3268
                     /gene="amn"
                     /locus_tag="ACHKBL_004681"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008714 - AMP nucleosidase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0044209 - AMP salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HFO5821425.1"
                     /translation="MNNKGSGLTPAQALDKLDALYEQSVVALRNAIGKYITSGELPDE
                     NARKQGLFVYPSLTVTWDGSTTNPPKTRAFGRFTHAGSYTTTITRPTLFRSYLNEQLT
                     LLYQDYGAHISVQPSQHEIPYPYVIDGSELTLDRSMSAGLTRYFPTTELAQIGDETAD
                     GIYHPTEFSPLSHFDARRVDFSLARLRHYTGTPVEHFQPFVLFTNYTRYVDEFVRWGC
                     SQILDPDSPYIALSCAGGNWITAETEAPEEAISDLAWKKHQMPAWHLITADGQGITLV
                     NIGVGPSNAKTICDHLAVLRPDVWLMIGHCGGLRESQAIGDYVLAHAYLRDDHVLDAV
                     LPPDIPIPSIAEVQRALYDATKLVSGRPGEEVKQRLRTGTVVTTDDRNWELRYSASAL
                     RFNLSRAVAIDMESATIAAQGYRFRVPYGTLLCVSDKPLHGEIKLPGQANRFYEGAIS
                     EHLQIGIRAIDLLRAEGDRLHSRKLRTFNEPPFR"
BASE COUNT          783 a          822 c          804 g          953 t
ORIGIN      
        1 tgttttttca atattgaaat tataagcttt ataatcgtag taactaaatg caatacgtct
       61 caacaatgat gctaaaaaca tacctaacct cgcctcccta ctggttataa tgcaatgcag
      121 tctatcagac tcatcagggt gccattttgt gcatatgcgg acttttatgt ttcatatctc
      181 gaacctgtga ggcctctgct taatccttaa acaacaccag caactcctgc gctttcatct
      241 tccagcgaat ttttcatgtt gctgctcatc agccataaaa tcatttgcag atgcgctctg
      301 tcgaggtagt ctcataaggt tcgtttatag atcgacggca atgtgagtta ccttttccat
      361 actaattata aaaagacagt acaaacagga tcattatgga ctccacgctc atctccactc
      421 gtcccgatga agggacgctt tcgttaagtc gcgcccgacg agctgcgtta ggcagtttcg
      481 ctggtgccgt cgtcgactgg tatgattttt tactctatgg catcaccgcc gcactggtgt
      541 ttaatcgcga gtttttcccg caagtaagcc cggctatggg aacgctcgcc gcatttgcca
      601 cctttggcgt cggattcctg ttccgtccgc tcggcggtgt cattttcggt cactttggcg
      661 accgactggg acgtaagcgc atgttaatgc tgaccgtctg gatgatgggc atcgcgacag
      721 ccttgattgg tattcttcct tcattctcga ccattgggtg gtgggcacct attttgctgg
      781 tgacactgcg tgccattcag ggatttgccg tcggcggcga atggggaggc gcggcgttgc
      841 tttccgttga aagtgcgccg aaaaataaaa aagcctttta cagtagcggt gtacaagttg
      901 gctacggtgt aggtttactg ctttcaaccg gactggtttc attgatcagt atgatgacaa
      961 ctgacgaaca gtttttaagc tggggctggc gcattccttt cctgtttagc atcgtactgg
     1021 tactgggagc attgtgggtg cgcaatggca tggaggagtc cgcggaattt gaacaacagc
     1081 aacataatca agcggccgcg aaaaaacgca tcccggttat cgaagctctg ttacgacatc
     1141 ccggtgcttt cctgaagatt attgcactac gactgtgcga gttgctgacg atgtacattg
     1201 ttactgcctt tgcacttaat tattcaaccc agaatatggg gttaccgcgc gaacttttcc
     1261 ttaatattgg tttgctggta ggtggattaa gctgcctgac aattccctgt tttgcctggc
     1321 ttgccgatcg ttttgggcgg cgcagggttt atatcacagg tgcgttaatc ggaacgttga
     1381 gcgcatttcc tttctttatg gcgcttgaag cacaatctat tttctggata gttttcttct
     1441 ccatcatgct ggcaaacatt gcgcatgata tggtggtgtg tgtacaacaa ccgatgttta
     1501 ccgaaatgtt tggtgccagt tatcgctata gtggcgctgg agtcggttat caggttgcca
     1561 gtgtggttgg cggtggattt acacctttta ttgccgctgc actcatcact tactttgccg
     1621 ggaactggca tagcgtcgcc atttatttgc tggctggatg cctgatttcc gcaatgaccg
     1681 ctttgttgat gaaagacaat caacgcgctt aatagcctgg cgaagatcat ccgatcttcg
     1741 ccttacactt ttgtttcaca ttcctgtgac atactatcgg atgtgcggta attgtatgga
     1801 acaggagaca cacatgaata ataagggctc cggtctgacc ccagctcagg cactggataa
     1861 actcgacgcg ctgtatgagc aatctgtagt cgctttacgc aacgccattg gcaaatatat
     1921 tacaagtggc gaattacctg atgaaaacgc ccgcaaacaa ggtctttttg tctatccatc
     1981 actgaccgta acctgggacg gtagcacaac caatcccccc aaaacgcgcg catttggtcg
     2041 ttttacccac gcaggcagct acaccaccac gattactcgc cctactctct ttcgttcgta
     2101 tctcaatgaa caacttacgt tgctgtatca ggattatggt gcgcatatct cggtgcaacc
     2161 ctcgcagcat gaaatccctt atccttatgt catcgatggc tctgaattga cacttgatcg
     2221 ctcaatgagc gctgggttaa ctcgctactt cccgacaaca gaactggcgc aaattggcga
     2281 tgaaactgca gacggcattt atcatccaac ggaattctcc ccgctatcgc attttgatgc
     2341 gcgccgcgtc gatttttccc tcgcgcggtt gcgccattat accggtacgc cagttgaaca
     2401 ttttcagccg ttcgtcttgt ttaccaacta cacacgttat gtggatgaat tcgttcgttg
     2461 gggatgcagc cagatcctcg accctgatag tccctacatt gccctttctt gtgctggcgg
     2521 gaactggatc accgccgaaa ccgaagcgcc agaagaagcc atttccgacc ttgcatggaa
     2581 aaaacaccag atgccagcat ggcatttaat taccgccgat ggtcagggta ttactttggt
     2641 gaatattggc gtgggaccgt caaatgctaa aaccatctgc gatcatctgg cagtgctacg
     2701 cccggatgtc tggttgatga ttggtcactg tggcggatta cgtgaaagtc aggccattgg
     2761 cgattatgta cttgcacacg cttatttacg cgatgaccac gttcttgatg cagttctccc
     2821 gcccgatatt cctattccga gcattgcgga agtgcaacgt gcgctttatg acgctaccaa
     2881 gttagtcagc ggcaggcctg gtgaagaagt caaacagcgg ttacgtactg gcacggtggt
     2941 aaccacagat gacaggaact gggagttacg ttactcagct tctgcacttc gttttaactt
     3001 aagccgggcc gtagcaattg atatggaaag tgcaaccatt gccgcgcaag gatatcgttt
     3061 ccgcgtgcca tacgggacac tactgtgtgt ttcagataaa ccgttacatg gtgagattaa
     3121 acttcccggc caggctaacc gtttttatga aggcgctatt tccgaacacc tgcaaattgg
     3181 cattcgggcg atcgatttgc tgcgcgcaga aggcgaccga ctgcattcac gtaaattacg
     3241 aacctttaat gagccgccgt tccgataata agttatgaac cttcttatca gaaatagatg
     3301 tgaaggatta agtaagacat ctggcaaata gcctgcaata aacgctggct gtttgcttcc
     3361 tt
//
LOCUS       DAZFLD010000323         3329 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.325, whole genome shotgun sequence.
ACCESSION   DAZFLD010000323 DAZFLD010000000
VERSION     DAZFLD010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3329
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.325"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(119..2338)
                     /locus_tag="ACHKBL_004682"
     CDS             complement(119..2338)
                     /locus_tag="ACHKBL_004682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001282582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VapE domain-containing protein"
                     /protein_id="HFO5821426.1"
                     /translation="MPDNKPWGATPDEWFHFDLVLGMTDRLLPVVSNPCAELSPNSKL
                     SHNFGKTPSRYNGQRKVVGIPDWANYVVTENDFSRWSNEPDYGICVRTGDGVVALDCD
                     INDAGMQEIVRNIILSCLGELPPRRWRADSHKCLYLIAVDGDYRKRGHRLEGENKQIE
                     LLAKGQQFVACGTHPAGERIQWDCGLPGEPLKITSEQLESLWQRLADNLPVKDSYTAG
                     AGRQRDLSCVDPSATDDVADWLDANGWTLSVSSDGSRNLKPFRDESEYSDGCSETSIK
                     YFPKGTGGFEQGHFKSMHNTDAGLTDADWLDAYGYRVADFEDLTVAEDGDKPEYLPWP
                     KFSRDKNDRIEGTVTNVLLALRRPDLCGVQIRLDEFRDEIILTTPRGKNLRLRDEFYT
                     MLHSRLECLGFKKFEEAAIRRAVRLVAYENRFDSLKDWIANLPAWDGVPRIDTFFCRH
                     WGIEANAYTHAVGRYWWTLLAGRALVPGIKGDMAVILVSQQGKNKSEGIRAMAPTPEH
                     YMELDFGKKGEERIREMRGHNVIELGEMRGMDKRGIGDVRVTISTRADRNRGLFREHY
                     VTLPRRCGFIGTSNSDTPLTDTEGNRRWLPMTIPDETDGKFIAQGIADEKIQLWAEAV
                     AVFKRDGIAWENAETLAKSILGDYEVKDEVWMSCVAEWLELPQDLYDMAADGTKKNCE
                     RVPLTSKDVLVEAIGFKAPQVKRSDEMKIAAIMKKLGYKNKQLQSLPGKPRSWEKCK"
     gene            complement(2342..2566)
                     /locus_tag="ACHKBL_004683"
     CDS             complement(2342..2566)
                     /locus_tag="ACHKBL_004683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015571354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO5821427.1"
                     /translation="MTSSTLASRIKERRKALGITQTSLAESVGMRQQSVQYLESGRAT
                     RTGFILELAKVLKCDPDWLLNGENSEQQKV"
     gene            2674..3303
                     /locus_tag="ACHKBL_004684"
     CDS             2674..3303
                     /locus_tag="ACHKBL_004684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000049986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family protein"
                     /protein_id="HFO5821428.1"
                     /translation="MNLANRAKKRRTELNLTQVEVAKRAGISQQSIEAIENGKTLKPR
                     NLLALASALECDPKWLLLGGDVVTDFNYGARRVPVLSYVQAGAFTSAELLREEGDFEY
                     ILTTAELSESSFALRIRGDSMEPEFKEGDIVIIDTDVYPTPGEFVAACNGSHEATFKK
                     YRPVGIGAKGEEDFELVPLNSDYPICRSWEKPIKIIGTMVEHRIFRRKR"
BASE COUNT          851 a          872 c          773 g          833 t
ORIGIN      
        1 ttgagtaggt tgagtggttg agtaatatca tgattttaat accatttttc ttactcaacc
       61 tacccttgtg aggttgagta ggttgagtaa caacctgcaa aaatctacaa acttgtagtt
      121 atttgcattt ttcccagctg cgaggcttac ccggtaaact ttgcaactgt ttatttttgt
      181 aaccaagctt cttcatgatt gctgcaattt tcatttcgtc actgcgtttt acctgggggg
      241 ctttgaagcc aatcgcctcg accaatacgt cttttgacgt aagggggacg cgttcgcagt
      301 tctttttcgt gccgtcggca gccatatcgt acagatcctg tggcaactct aaccactcag
      361 cgacgcatga catccagacc tcatctttga cctcataatc cccaagaata gacttagcca
      421 gtgtttcagc gttctcccat gcgatgccat cacgtttaaa aacggcgact gcttcagccc
      481 aaagctgtat tttttcgtca gcgataccct gagcgataaa cttgccgtca gtctcatccg
      541 gtatcgtcat cggcaaccaa cggcggttac cttcggtatc tgtcaatggc gtatcggagt
      601 tagaagtacc aataaaaccg caacgcctcg gtaaagtgac atagtgctcc cgaaacaacc
      661 cgcggttgcg gtctgcacgg gtggatatgg tgacacgaac atcaccaata ccccgtttat
      721 ccataccgcg catctcacca agctcaataa cgttatgccc tcgcatctca cgaatgcgct
      781 cctctccctt cttaccgaag tccagttcca tgtaatgctc aggggttgga gccattgcac
      841 ggataccttc ggacttgttc ttaccctgtt ggctgacaag gatcacagcc atgtcccctt
      901 taatgccagg aaccagagct cgacccgcga gcaaagtcca ccagtagcgc ccgactgcgt
      961 gggtgtaggc gttggcctcg ataccccagt gccgacagaa gaaagtatct atacggggaa
     1021 caccatccca tgcaggaaga ttggcaatcc agtctttaag cgaatcaaaa cggttttcat
     1081 acgcgaccag gcgcaccgca cgcctgatcg ctgcctcttc aaatttcttg aatcccagac
     1141 attcgagcct agagtgcagc atggtataaa actcatcacg caaccgcagg tttttccccc
     1201 gtggagtggt cagtattatc tcgtcccgga actcatcaag gcggatttgt acaccgcaga
     1261 gatccggtcg ccgcagcgcc agcagcacat tggtaacggt accttctatc cggtcattct
     1321 tatcacgact gaacttaggc cagggaagat actcaggctt atcgccatct tcagccactg
     1381 tcaaatcttc gaagtccgcc acgcggtagc cgtatgcatc cagccagtcg gcatcagtaa
     1441 gccccgcatc ggtgttgtgc atgcttttaa aatgtccctg ttcaaatccc cccgtccctt
     1501 ttgggaaata cttaatggat gtttcgctgc aaccgtcgct gtattctgac tcatcccgaa
     1561 acggtttcag attgcgggaa ccatcagagc tgactgacag agtccacccg ttcgcgtcaa
     1621 gccagtcggc gacatcatct gtcgcggaag gatcaacaca ggaaaggtca cgctgacgtc
     1681 ctgcacctgc ggtatagctg tctttcacgg gtaagttatc cgccagacgc tgccacaggc
     1741 tttcgagctg ttcagatgta attttcagag gttcgccggg caaaccgcag tcccactgaa
     1801 tacgttcgcc tgccggatgg gtaccacaag ccacaaactg ctggccctta gcgagcagct
     1861 ctatttgttt gttttcgcct tccaggcggt gaccacgttt acggtagtcg ccatcaacag
     1921 caatcaggta caggcattta tgactgtcag cccgccagcg acgtggcgga agttccccca
     1981 ggcatgacag gataatattg cgtacgatct cctgcatccc ggcgtcattg atatcgcagt
     2041 ccagagccac caccccgtcg ccagtacgaa cgcaaatacc gtaatcgggt tcgttcgacc
     2101 accgggaaaa atcgttttcc gtaacaacat agttagccca gtcggggata ccaacaacct
     2161 tacgttggcc attgtagcga ctgggagttt tgccgaaatt atgagacagc ttgctattag
     2221 gcgacagttc tgcacaggga ttggacacca caggtaaaag cctgtctgtc atccccaaca
     2281 ccagatcgaa gtggaaccac tcatcgggcg tagctcccca gggtttgtta tcaggcatgg
     2341 gttacacctt ttgctgctct gaattttcgc cgtttaatag ccagtcagga tcgcatttca
     2401 gaacttttgc caattcaagg atgaaaccgg tacgggtggc gcgtcctgac tcaaggtatt
     2461 gaacagactg ctggcgcatg ccaacacttt cagccaatga agtttgggtt atacctaaag
     2521 ctttgcggcg ctcctttata cgtgaagcaa gggtagaact ggtcattgag taagccctca
     2581 ttattacagg tttacttgta attacctcac aggcaacctt gtttgtcaaa tacagaagta
     2641 tctgtaatca tcaatgacag aaaaggagtc aacatgaatc ttgccaaccg agctaaaaaa
     2701 cgcagaacag aactaaactt aactcaggtc gaagttgcga aacgagcggg cattagccag
     2761 cagtcgattg aagctataga gaacgggaaa acacttaaac cacgcaatct tcttgcttta
     2821 gcgtcagctc ttgaatgcga tcctaaatgg ctacttcttg ggggcgatgt tgttacagat
     2881 ttcaactacg gagcccgaag ggtgcctgtt ctaagctacg tacaagcagg ggctttcaca
     2941 agcgctgagc tacttcgtga agaaggtgat ttcgaataca ttctcaccac ggctgagcta
     3001 tcagaaagtt catttgcgct acgtatacgt ggggattcaa tggaacctga atttaaagag
     3061 ggggacatcg ttataattga cactgacgta taccctactc ctggtgaatt cgttgcggca
     3121 tgtaacggta gccacgaagc aacgttcaaa aaataccgcc cggtagggat tggcgctaag
     3181 ggtgaggaag actttgaact tgttccattg aatagtgatt accctatttg tcgctcctgg
     3241 gaaaaaccaa ttaagatcat agggacaatg gtagaacatc gcatattccg cagaaaaaga
     3301 taatcgcatc ctgactacta aacaaagag
//
LOCUS       DAZFLD010000324         3282 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.326, whole genome shotgun sequence.
ACCESSION   DAZFLD010000324 DAZFLD010000000
VERSION     DAZFLD010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3282
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.326"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..354
                     /gene="radC"
                     /locus_tag="ACHKBL_004685"
     CDS             <1..354
                     /gene="radC"
                     /locus_tag="ACHKBL_004685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFO5821429.1"
                     /translation="AAREWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTE
                     VHPREVIKRALYHNAAAVVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLI
                     VGGNQVFSFAEHGLL"
     gene            417..638
                     /locus_tag="ACHKBL_004686"
     CDS             417..638
                     /locus_tag="ACHKBL_004686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HFO5821430.1"
                     /translation="MKIITRGEAMRIHQQHPASRLFPFCTGKYRWHGSTEAYTGREVQ
                     DIPGVLAVFAERHKDSFGPYVRLMSVTLN"
     gene            801..1175
                     /locus_tag="ACHKBL_004687"
     CDS             801..1175
                     /locus_tag="ACHKBL_004687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HFO5821431.1"
                     /translation="MSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLHYL
                     ADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSGELNPRNQHTVTLYAKGLTCEAD
                     TLGSCGYVYLAVYPAQAPATTS"
     gene            1222..1599
                     /locus_tag="ACHKBL_004688"
     CDS             1222..1599
                     /locus_tag="ACHKBL_004688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TA system toxin CbtA family protein"
                     /protein_id="HFO5821432.1"
                     /translation="MKTLSDTHVREVSRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPEFSACTRSQLINSIDILRARRATG
                     LMTRDNYRTVNNITQGKHPEAKQ"
     gene            1596..2084
                     /locus_tag="ACHKBL_004689"
     CDS             1596..2084
                     /locus_tag="ACHKBL_004689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
                     /protein_id="HFO5821433.1"
                     /translation="MKLTLTLEADNVNVQALNMGRIVVDVDGVNLAELINVVCDNGHS
                     LRVVDESDRVSTDCTPPFAALTGIRCSTAHITETDNAWLYSLSHQTNDDCETEWIHFT
                     GSGYLLRTDAWSYPVLRLKRLGLSKTFRRLVVTLTRRYGVSLIHLDASAECLPGLPTF
                     NW"
     gene            2096..2293
                     /locus_tag="ACHKBL_004690"
     CDS             2096..2293
                     /locus_tag="ACHKBL_004690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HFO5821434.1"
                     /translation="MKSLTTETALDILIAWLQDNIDCESGIIFDNDEDKTDSAALLPC
                     IEQAREDIHTLRQVQLLHQNR"
     gene            2378..3223
                     /locus_tag="ACHKBL_004691"
     CDS             2378..3223
                     /locus_tag="ACHKBL_004691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001564112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HFO5821435.1"
                     /translation="MSAITVSRPEVVTGHTDVICSTSVSHILAVRKTTLLQIDTLIRQ
                     LAEISVLTESIGGKTALDWAMKQDFRCGCWLMEKPETAMKAITRNLDREIWRDLMQRS
                     GMLSLMDAQARDTWYRSLEYDNFPEISEANILSTFEQLHQNKDEVFERGVINVFRGLS
                     WNYKTNCPCKFGSKIIVNNLVRWDWWGFHLITGQQADRLADLERMLHLFSGKPIPDNR
                     ENITLRLDDHIQSVQGKECYEDEMFSIRYFKKGSAHITFKNQELVDRLNDIIARHYPR
                     MLPSL"
BASE COUNT          828 a          887 c          808 g          759 t
ORIGIN      
        1 gcggcacggg aatggctgat tctgaacatg gcgggactgg agcgtgaaga attccgggtg
       61 ctgtatctga ataaccagaa tcagttgatt gccggtgaaa ccctcttcac cggcaccatc
      121 aaccgcacgg aagtccatcc ccgggaagtg attaaacgcg ccctgtacca caatgccgct
      181 gccgtggtgc tggcgcacaa tcacccgtcc ggtgaagtca cacccagcaa ggcagaccgc
      241 cttatcacgg aacgtctggt acaggcactg ggcctggtgg atatccgggt gccggaccat
      301 ctgatagtcg gtggcaacca ggttttctcc tttgccgaac atggtctgct ttaacccgtc
      361 acaaccacat cacgcttgtt ttcactttta tcttctgtct tcagaggtat cacactatga
      421 aaatcatcac ccgtggtgaa gccatgcgta ttcaccaaca acatccggca tcccgtcttt
      481 ttcctttctg taccggcaaa taccgctggc acggcagcac tgaagcgtat accggtcgtg
      541 aagtgcagga tattcccggt gtgctggccg tgtttgctga acgccataag gacagttttg
      601 gcccgtatgt ccggctgatg agcgtcaccc tgaactgatg ttccgtcacc cggaaaacga
      661 gtacgaccag atggactcag acgccgctgt ggatgaggcc ctgctcatca acgaatacac
      721 ctttaccgaa gcaggtcagc gcgccggctg acctgtcatc gttaaaccat tcttaaccac
      781 ttttcagaga ggattttatc gtgtcagaca aactctccgg gataactcat cccgacgaca
      841 atcacgaccg cccctggtgg gggcttccct gcacagtgag gccctgtttt ggtgcccgtc
      901 tggtgcagga gggtaaccgc ctgcattacc ttgccgaccg cgccggtatc agaggccgct
      961 tcagcgacgc agatgcatac catctggacc aggcctttcc gctgctgatg aaacaactgg
     1021 agctcatgct caccagcggt gaactgaatc cccgaaatca gcataccgtc acgctgtatg
     1081 caaaagggct gacctgcgaa gccgacaccc tcggctcctg tggttacgtt tatctggctg
     1141 tttatccggc acaggcaccc gcaaccacct cataaccccg aagctctcac ctttagcact
     1201 accagttaca gagaacttaa gatgaaaaca ttatccgaca cacatgtacg ggaggtatct
     1261 cgctgcccgt ctcccgtcac catctggcag acactgctca tccgactgct ggaccagcat
     1321 tatggcctca cactgaatga cacaccgttt gccgatgaac gtgtgattga gcagcatatt
     1381 gaagctggca tttcactgtg cgatgcagtg aactttcttg ttgaaaaata cgcactggtg
     1441 cgtaccgacc agccggaatt cagtgcctgt acccgttctc agttaataaa cagcatcgat
     1501 atcctccggg ctcgcagggc gaccggcctg atgacccgcg acaattacag aacggtaaat
     1561 aacattaccc aggggaagca tccggaggcg aaacaatgaa actgactctg acgctggaag
     1621 cagacaacgt taacgtgcag gcgctgaaca tgggacgaat tgtcgttgac gtcgatggtg
     1681 ttaatctcgc tgaactgatt aacgtggtct gcgacaatgg ccactccctt cgtgttgttg
     1741 atgaatctga ccgggtctca acagactgca cgccaccatt tgctgccctt accggcatac
     1801 gctgcagtac cgcacatatc acggaaacgg acaacgcctg gctgtactcg ctgtcacacc
     1861 agactaatga cgactgcgaa acagaatgga ttcattttac aggcagcgga tatctgttac
     1921 gtaccgatgc gtggtcatac ccggttctgc ggcttaagcg cctgggcctg tcaaaaacgt
     1981 tccgtcgtct ggttgtcaca cttacccgac gttatggcgt cagtctcatt catctggatg
     2041 ccagcgctga atgcctgccg ggtttaccca ctttcaactg gtaaccagga acaacatgaa
     2101 atcattaacc acggaaacag cactggatat tctgattgcg tggctgcagg acaatatcga
     2161 ctgcgaatcg ggaattatct ttgacaacga tgaggataaa acggattcgg cagcactgct
     2221 gccctgcatt gaacaggcga gggaggatat ccataccctg cgccaggtgc agcttctgca
     2281 ccagaaccgg tgagtcttac tcaccagact tcattccact gacgccagcc tgaacagggc
     2341 tggcgttttc atttatctgc gaaaaggaat atcgattatg tctgcaatca cagtctcccg
     2401 tccggaagtg gtcaccgggc atacggacgt tatctgctcc acctcagtca gtcacattct
     2461 ggctgtgcga aagactacac tgctgcaaat cgacacactt atccggcaac tggctgaaat
     2521 ctcggtactg acagaaagta ttggaggtaa aactgctctg gactgggcca tgaaacagga
     2581 ttttcgctgc ggctgctggc tgatggagaa acctgaaacc gcaatgaaag ctatcacacg
     2641 caatctcgat cgtgaaatct ggcgtgacct gatgcaacgt tccgggatgc tttccttaat
     2701 ggatgcacag gcccgtgata catggtaccg gtcactggaa tatgataatt ttccggaaat
     2761 cagcgaggcg aatatcctga gcacatttga acaactgcac cagaataagg atgaggtgtt
     2821 tgagcgaggg gtgatcaacg tcttcagggg actgagctgg aattacaaaa ccaattgccc
     2881 ctgcaaattt ggcagtaaaa ttatcgtcaa caacctggtg aggtgggact ggtgggggtt
     2941 tcatcttatc accgggcaac aggcagatcg acttgccgat ctggaaagaa tgctacacct
     3001 gttcagcggc aaaccgatcc ccgacaaccg ggaaaacatc acccttcgtc tggatgatca
     3061 catccagtct gttcagggta aagagtgcta tgaagatgag atgttcagca tcagatactt
     3121 taagaagggc tccgcgcaca tcacgttcaa aaatcaagaa ctggttgaca ggctgaatga
     3181 cattattgca agacactatc cgagaatgtt accttcatta taaagttcta agaatgaatt
     3241 caattttgca ttttagcttc attccctcac gcaaaattat tt
//
LOCUS       DAZFLD010000325         3200 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.327, whole genome shotgun sequence.
ACCESSION   DAZFLD010000325 DAZFLD010000000
VERSION     DAZFLD010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3200
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.327"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            4..1554
                     /locus_tag="ACHKBL_004692"
     CDS             4..1554
                     /locus_tag="ACHKBL_004692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HFO5821436.1"
                     /translation="MKTVFTTLSVIFGVIFSHSVLAQDVTIYYTNDIHAHVNPAKIIA
                     VDKNRLIGGMANISGIVNEAKKKSKDVFFFDAGDYFTGPYISTLTKGEAIIDIMNTMP
                     FDAVSVGNHEFDHGVPNMVSQLSKAKFPILLGNIYYTDTNKPVWDHPWTIIEKDGLKI
                     GVIGLHGAFAFYDTVAAKAREGVEARDEIKYLNKALAELKGKVDITVLLIHEGVPARQ
                     SSFGSKDVERLLQADIETAKKVNGVDVLITGHAHVGTPQPIKVNNTLIVSTDAYGTNI
                     GKLVLDYNPKTKKIDSYNGELITIFADQFKPDTIVQNTIDKWSAKLNKITQEVVGHSP
                     VVLTREYGSSSSTGNLILDAMMEKTPDAVAGFQNSGGIRADFPKGDITLGDVISTFPF
                     NNDLIEMDLTGRDLKSLMTHATNLTNGVLQVSKSVAVVYDSKKPLNQRLISFTINGKP
                     VEDNQTYRIATHSFCASGGDGFEAFLNGKNVKTIPGTTSAESIIDYFKNHKPVTPDLT
                     KRVMDVAK"
     gene            1595..>3200
                     /locus_tag="ACHKBL_004693"
     CDS             1595..>3200
                     /locus_tag="ACHKBL_004693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001357039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HFO5821437.1"
                     /translation="MKTAQRTLFAATLLTIFSTSVMAQDVTIIYTNDLHAHVDSYKVP
                     YVADGKRDIGGFANISTLVKQEKAKNKATFYFDAGDYFTGPYISSLTKGEAIIDIMNT
                     MPFDAVSIGNHEFDHGWDNALRQLSKANFPVLLANVYHKESEKPFWNKPYTILEKDGI
                     KIGIVGLHGVFAFNDTVSELSLQGLDNDNNNRFDKSSATLKNQGIEARDEVKYLQHYI
                     DELRDKVDLTVALVHEGVPARQSSIGNTDVRRALDKDIQTASKVKGLDILITGHAHVG
                     TPEPIKVGNTLILSTDSGGIDIGKLILDVNPTARTHKVKSFELKTVYADEWIPDPTTQ
                     KVINGWNKKLADLVRQPVGESSIALTRAYGESSQLGNLFTDAMLVAAPTAQIALINSG
                     SLRADINAGTITFGDITSTFPFKNELTEMDLSGKDLRNLLEHGASLTNGILQMSKGAE
                     MRYTPKKPVGQRIVSFKINGEEIVDTNIYHVATTTFLALGGDGFLAFKEGKNVQVRAG
                     NNMSDVVIDYLKKGHKITPAQVNEMRV"
BASE COUNT          990 a          669 c          694 g          847 t
ORIGIN      
        1 atgatgaaaa ctgtatttac aacattatca gtcatattcg gcgtgatatt ttctcacagt
       61 gttctagctc aggacgttac tatttattat acgaatgaca ttcacgcaca tgtcaatcca
      121 gcaaaaatca tcgctgttga taagaacaga cttataggtg gtatggctaa tatttcaggc
      181 atcgttaatg aagcgaagaa aaaaagcaaa gatgtgtttt tctttgatgc aggcgactat
      241 tttaccgggc cgtatatcag caccctgacc aaaggtgagg ctattattga tatcatgaac
      301 actatgccct ttgatgcggt gtctgttggt aatcacgagt tcgatcacgg tgtgcccaat
      361 atggtgtctc agttatcaaa agcaaaattc cccattctgc tgggaaatat ctactacacc
      421 gatacgaata aaccagtttg ggatcatccg tggaccatta tcgaaaaaga tggtttaaag
      481 attggtgtga ttggattgca tggcgcattc gctttctatg atacagtcgc ggcaaaagca
      541 cgtgaaggcg tagaagccag agacgaaatt aaatatttaa ataaagcact tgcagaatta
      601 aaaggtaaag tcgacattac cgttttgcta attcacgaag gtgtcccggc gcgtcaatca
      661 agctttggta gtaaagatgt ggagcggcta ctacaggcag atattgagac agctaaaaaa
      721 gtgaatggtg ttgatgtctt aattaccggt cacgcacatg tcggaacacc gcaaccaata
      781 aaagttaata acacattaat tgtatctacc gatgcatacg gcaccaacat cggaaaactt
      841 gtgcttgatt acaatccgaa aacgaagaaa attgacagtt ataatggtga gttaatcacc
      901 atctttgcag atcaatttaa acctgacacc atcgttcaaa atactattga taaatggagt
      961 gcaaagctta acaaaataac acaggaagtt gtcggccatt ctcccgtagt tttaacacgt
     1021 gagtatggta gttcctcttc taccggcaac cttattcttg atgcaatgat ggaaaaaaca
     1081 cctgatgccg ttgccggatt tcaaaatagc ggtgggattc gagctgattt tcctaaaggc
     1141 gatatcacac tgggagatgt tattagcaca ttccccttta ataatgacct catcgagatg
     1201 gatttgacgg gccgcgatct caaatcattg atgacgcatg caaccaatct aactaacggt
     1261 gtgttacaag tttctaaaag tgttgcggtt gtctatgaca gcaaaaaacc cctcaaccaa
     1321 cggttaatct ctttcaccat taacggcaaa cccgtggaag ataatcaaac atatcgtatt
     1381 gccacgcact ccttttgtgc cagtggtggt gacggttttg aagcattttt gaatggaaaa
     1441 aatgtgaaga cgataccggg aacaacctcg gcggaatcta tcatcgatta tttcaaaaat
     1501 cataaacctg tcaccccaga cttaactaaa cgagtcatgg acgtcgccaa ataaaatctc
     1561 atttatcagt caatttattt aacaaggaat gcgaatgaaa acagcgcagc gtactttatt
     1621 tgccgcaaca ttgctgacta ttttttcgac ctcagttatg gcgcaggatg tcacgattat
     1681 ttataccaat gatcttcacg ctcatgtgga ttcctataaa gtaccgtatg tcgcagacgg
     1741 aaaacgcgac attggtggct ttgccaatat atccacgtta gtaaaacaag agaaagccaa
     1801 aaataaagcc accttttatt ttgatgctgg agactatttc accgggccat atatcagtag
     1861 cctgacaaag ggcgaagcaa taattgatat tatgaatacc atgccttttg acgcagtatc
     1921 aatcggtaat catgagtttg accatggctg ggataatgcc ctccgacaat taagcaaagc
     1981 gaatttccct gttttattag ccaatgttta tcataaagag agtgagaaac ccttctggaa
     2041 caagccctac accatcctgg aaaaagatgg catcaagatt ggtattgttg ggctacacgg
     2101 cgtatttgca tttaatgaca cggtctcgga gctctctctc cagggactcg ataatgacaa
     2161 taataaccga tttgataaat cttccgcgac cctcaagaat caggggattg aagcacgtga
     2221 tgaagtgaag tatttgcagc attatatcga tgagttacgt gataaggttg acctcaccgt
     2281 ggcgctggtt catgaaggcg taccggcgcg tcagtccagc attggcaata cagatgttag
     2341 acgcgctctt gataaagata ttcaaaccgc aagcaaggtg aaaggattgg atattctgat
     2401 taccgggcat gcccacgtcg gaacgccaga accaatcaaa gtcggcaaca ccttaatcct
     2461 ttcgactgac agtggcggta tcgatattgg taaattaatc cttgatgtca accctacagc
     2521 tcgtactcat aaagtgaaga gctttgagct gaaaacggtt tatgccgatg aatggatacc
     2581 tgatccaacc acacaaaaag ttatcaatgg ctggaataaa aaactggcgg atcttgtacg
     2641 ccaaccagtc ggtgaatcat cgattgcatt aactcgtgcc tacggtgagt catcgcaact
     2701 tggcaacctg tttaccgatg caatgcttgt cgctgcaccg actgcacaga ttgcattgat
     2761 aaactccggc agcttacgtg cggatataaa tgcaggcacg attacctttg gcgacattac
     2821 cagtacattc cccttcaaga atgaacttac tgaaatggat ctcagcggta aggatctgcg
     2881 taacctactg gaacacgggg catcgcttac caatggcatc ctgcaaatgt caaaaggcgc
     2941 agaaatgcgc tataccccga agaaaccagt aggtcagcgc atagtatctt ttaaaattaa
     3001 tggtgaagag atcgttgata cgaatattta ccatgttgca acaacgactt tccttgcact
     3061 ggggggcgat ggttttctgg cattcaaaga agggaaaaat gttcaggtcc gcgccggaaa
     3121 taacatgtcc gatgtcgtga tcgattattt gaagaaaggc cacaagatta cgcctgcaca
     3181 ggtaaatgaa atgcgggtgg
//
LOCUS       DAZFLD010000326         3164 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.328, whole genome shotgun sequence.
ACCESSION   DAZFLD010000326 DAZFLD010000000
VERSION     DAZFLD010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3164)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3164)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3164
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.328"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(144..2012)
                     /locus_tag="ACHKBL_004694"
     CDS             complement(144..2012)
                     /locus_tag="ACHKBL_004694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5821438.1"
                     /translation="MGMFNYKIYNAAHVTYLVSSSYKLAIYSNTGKVLGLGSFLASKL
                     FAKHPDKIDYSPDALNEGWRDITVEELRLSSEYVDSRGFYRFPSPVTGNPASGFGPQL
                     KVVGLFDAQQNLQKIAISFCGTNDICDIVDYTQLNNGKALSEAENLLQSIRDFAISNG
                     LSGEDVIVTGYSLGGGFTNIMARLSESLADGFYANSDYIAWASPLIFDGDGKVLNIGF
                     ENDPVFRVLGDESGFWEAVKAMGPGLTTPDREYDSSPDNIVLYTDLYGSFLWNIIPFS
                     VLALPLGSWSAHFAGSKTDAFERILGSQFYDYMSPDSTVIVDEQSGWTSWYSWVHDKG
                     DDSRERPVFIIGNDGNNLLAGGQGGDYIEGAGGNDKIEPGKGANRVDGGSGYDTLLLG
                     DVMDDWNIYRVSDGTLFFHSERGAGLTEATGIEGISFSGDWRSKLSPYQIEEEQITDH
                     RYLLKWRNKNVDYGKHTEGTNGDDSLFGNIIFGLDGNDILHSAGGEGVSLLHGGRGND
                     YLHGASGNDELYGAEGNDWLYGGGGNDYLYGGTGHDVFVFDAACEGNSFIMDFNKHKG
                     EKDFLFLSKDIVTSTDEFISCLKQTGNDVEAGIAGGQIYFVNTALEDINHNSVYIV"
     gene            complement(2091..>3164)
                     /locus_tag="ACHKBL_004695"
     CDS             complement(2091..>3164)
                     /locus_tag="ACHKBL_004695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5821439.1"
                     /translation="KADAEEAAEARVFYEKAFSNVYQTDDLYARTDTTSLFAPAAIKL
                     AKSTAQWATILEKNAGAQMSQDQNAGGETRYIYNGISGSDVITVGKSLGGTGLNMTAT
                     RNDMKVMTGDGDDIIITGQDYGRLASAGQWDYKYLTEMGNGNDTLIVGASNSNLNVIM
                     FNDGSIAAVKKDGAQLGSVIPFDSAYDTADGGNISGTTIDMGSGNDTVLALGHENGGT
                     AIINSTIKLGAGNDTIQINGDVKGGKSPSVITGDAGMDTLIISNGSVYSEHFSGFENI
                     ELGSKGEVKIVAADLVGKDSNSIQGGMLKIIGNSDSKVDLDGSDWIKGEIKNEGDITY
                     NVYTHASAPNISVLIEDKITQVI"
BASE COUNT          958 a          820 c          482 g          904 t
ORIGIN      
        1 aagcgcaata aattaaaatt aaaaaacaaa atcacttgca gtctattatc tttacgttcc
       61 tcaccgcaac cgcacacaaa aacaaaacac acaatgcatt gattttaaac aaaaaaacaa
      121 aacagaaact caacacataa aaatcagaca atataaacag aattgtgatt tatatcctca
      181 agcgctgtat taacgaaata tatctgtcct cctgcaatac ctgcctcaac atcatttccg
      241 gtttgtttca gacatgaaat aaactcatct gtcgatgtaa caatgtcctt tgataaaaac
      301 agaaaatctt tttcaccctt atgtttatta aaatccatga taaatgaatt cccttcacaa
      361 gcagcatcaa agacgaagac atcatgacca gtaccaccat acagataatc attacctccc
      421 ccaccatata accagtcgtt accttctgct ccgtacaact catcattacc agaagcccca
      481 tgaagataat cattcccgcg acctccgtgt aaaagactta ctccttctcc cccggcacta
      541 tgtaatatat catttccgtc aagaccaaaa ataatatttc cgaacagact atcatctccg
      601 tttgtacctt ctgtatgttt tccgtaatca acatttttat tacgccactt cagaagatac
      661 ctgtggtctg taatctgctc ttcttctatc tgataagggc ttagtttact cctccagtca
      721 ccagaaaaag agataccttc aatacctgtt gcctcagtaa gaccggctcc ccgttctgag
      781 tggaaaaaca gtgtcccgtc actgaccctg tatatattcc agtcatccat tacatcgcca
      841 agaagaagcg tgtcataccc actgccacca tcaaccctgt ttgctccctt acctggttca
      901 atcttatcat tccctcctgc cccctctata tagtcaccac cctgcccacc tgccagcaga
      961 ttattgccat cattaccgat gatgaataca ggtctttcac gactgtcatc ccccttgtca
     1021 tgaacccatg aataccagga tgtccagcca ctctgctcat ccacaatgac tgtactgtcc
     1081 ggtgacatgt agtcgtaaaa ctgagaccct aaaattcttt caaaggcgtc agtttttgaa
     1141 cccgcaaaat gcgcactcca gcttcccagc gggagagcaa gcacagaaaa ggggataata
     1201 ttccagagga atgaaccata aaggtctgtg tataaaacaa tgttatcagg agaagagtca
     1261 tattcacgat ccggggttgt caagcccggc cccattgctt taactgcctc ccagaacccg
     1321 gattcatctc ccagcacacg aaataccgga tcgttttcaa aaccgatatt taataccttc
     1381 ccatccccgt caaatatcag cggggaagcc caggctatat agtcagaatt agcataaaaa
     1441 ccatcagcca gagactcact gagtctggcc ataatgttag taaacccgcc ccccaggcta
     1501 tagcctgtga caataacatc ttcaccactt aaaccattac tgattgcaaa gtctctgata
     1561 ctttgaagca ggttttctgc ttcagacagc gcttttccat tattgagctg tgtataatca
     1621 acaatatcgc agatatcatt tgttccacag aatgatattg caattttctg tagattctgt
     1681 tgagcatcaa acaacccaac aaccttcaac tgaggaccaa atcctgacgc agggtttcct
     1741 gtaaccggac tgggaaagcg ataaaaacct cttgaatcga catattccga agataacctg
     1801 agttcttcca ctgtaatatc acgccagcct tcattaagag catcaggaga gtaatcaatt
     1861 ttgtctggat gcttcgcaaa tagtttagat gccagaaaac tgcccaatcc aagaaccttt
     1921 cctgtgttgc tatatatagc aagcttataa gaagaagaaa caaggtatgt tacatgcgca
     1981 gcattataaa ttttataatt aaacatcccc atacagactc ctttaaatta ccaaatctat
     2041 atattaagag gccacgcata cagcgcggcc ttgtcactat ttagctcaaa tcaaataacc
     2101 tgagttatct tatcttcgat taacacgctg atattaggtg cagatgcatg agtataaaca
     2161 ttgtaagtta tatcgccctc gttcttgatt tcacctttaa tccagtcact tccatccaag
     2221 tcaaccttag agtctgaatt acctatgatt ttcaacattc ctccctgaat ggagttacta
     2281 tcctttccta caagatctgc tgctactatt tttacttcac ccttactacc taactcaata
     2341 ttctcaaagc cagaaaaatg ttcagagtat acactaccat tactaataat caatgtatcc
     2401 ataccagcat caccagtgat gacagaagga gatttacctc ccttaacatc cccattgatc
     2461 tggatagtgt cattacctgc tccaagtttt attgttgagt taataatagc agttccacca
     2521 ttctcatgcc ccaaggctaa aacagtatca ttaccacttc ccatatcaat tgtagttcct
     2581 gatatattac ctccatcagc tgtgtcataa gcagaatcaa aaggaattac actccctaat
     2641 tgggcaccat cttttttaac tgcagcaata ctaccatcat tgaacataat tacattcaga
     2701 ttagagtttg atgccccgac aataagagta tcgtttccat tccccatctc tgtgagatat
     2761 ttataatccc actgaccagc acttgccaga cgaccataat cctgaccagt gataattata
     2821 tcatcgccat caccagtcat taccttcatg tcattacgcg tcgctgtcat atttaagcct
     2881 gtgccaccca agcttttgcc tactgtaata acatcacttc cggaaatacc gttatatatg
     2941 tatcgtgttt ctccccctgc attctgatct tgagacatct gagctccagc attcttctca
     3001 aggattgttg cccattgggc cgtggattta gccaatttta ttgccgcagg tgcgaataat
     3061 gatgttgtgt cagttctagc gtacagatca tcggtctgat acacatttga gaatgctttc
     3121 tcataaaaga ctctggcctc tgccgcttcc tctgcatccg cctt
//
LOCUS       DAZFLD010000327         3163 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.329, whole genome shotgun sequence.
ACCESSION   DAZFLD010000327 DAZFLD010000000
VERSION     DAZFLD010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3163)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3163)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3163
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.329"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            108..323
                     /locus_tag="ACHKBL_004696"
     CDS             108..323
                     /locus_tag="ACHKBL_004696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001498920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFO5821440.1"
                     /translation="MSQQKITGIDLAKTNFYLFSINAHGKPAGRIKLSRNQLLNWLVQ
                     QPKMTVAMEARGHPITGPGKSGNSTMM"
     gene            497..754
                     /locus_tag="ACHKBL_004697"
     CDS             497..754
                     /locus_tag="ACHKBL_004697"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5821441.1"
                     /translation="MERTQLINHVRGLLAEYGIVFSKGATELRQKLPALLEDAENELT
                     DTMKTLLHRQYIRLITLDNELEWYDSELKNMSAKILCANAC"
     gene            736..927
                     /locus_tag="ACHKBL_004698"
     CDS             736..927
                     /locus_tag="ACHKBL_004698"
                     /inference="COORDINATES: protein motif:HMM:NF014430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFO5821442.1"
                     /translation="MCKRLLAIPRFSPLVSQAIKSWMGDGKQFRRGRDASATLGLVPR
                     QFSTGGKQVLLGITKFGEM"
     gene            1069..2088
                     /locus_tag="ACHKBL_004699"
     CDS             1069..2088
                     /locus_tag="ACHKBL_004699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFO5821443.1"
                     /translation="MKYTPVGVDIAKHLIQVHFIDEHTGEVIDKQLRRQDFLMFFCNR
                     EPCLIGMEACRGSQHWGRELTKLGHKVRLLQAHFVKAFVMGNKNDVMDARAIWMAVQQ
                     PGKDIAVKTEEQQSVLVLHRSRRQLVKFRTMQINSLHGTLLEFGKTIHKGRAAMEREL
                     PEALERMKERLPPYLITVLENQYNRLNELDSLIEDIEKKLTSVARQNETCKRLLDIPG
                     VGPLIATAAVATMREASAFKSGREFAAYVGLVPKQTGSGGKIRLLEISKRGNTYLRAL
                     FIHSARAAALLPKEPGPWITELKKRRPTSVAIVAMANKLARTVWAIAAHGSKYDKNHV
                     SIRSY"
     gene            2809..3024
                     /locus_tag="ACHKBL_004700"
                     /pseudo
     CDS             2809..3024
                     /locus_tag="ACHKBL_004700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612066.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
BASE COUNT          967 a          680 c          787 g          729 t
ORIGIN      
        1 gtaacaggaa catcagtaag cgatgagatg gtcttccccc tccttcacga caacctttaa
       61 ttacgtcatg atggataaac tttaaccaca aaagtaagag ggataccatg tctcaacaga
      121 aaatcaccgg catcgatctg gctaaaacga acttttatct tttcagtatc aatgctcatg
      181 gcaaacccgc aggaaggatc aaactttctc gcaatcaact gcttaactgg ctggtgcagc
      241 agcctaaaat gactgttgcg atggaggctc gcgggcatcc catcactggg ccagggaaat
      301 caggaaactc gaccatgatg taatatttct tccggcgcaa catgttaaag cttatcagcg
      361 acgccagaaa aatgattatg acgacgctca ggcaatagct gaggcctgcc aacatggaac
      421 tatacgcccc gtaccaataa aaactctgga gtaacaggac gtccagactt ttttgaaaat
      481 gcgccggttg gtttcgatgg aaaggacaca actgataaat cacgttcgtg gacttcttgc
      541 tgaatatggc attgtgttca gtaaaggcgc aacagaatta cgccaaaaat taccggcatt
      601 acttgaggat gcggaaaacg aactcactga taccatgaag acgttattgc atcggcagta
      661 tatacggttg attacactag ataatgaact ggagtggtat gattctgaac tgaaaaatat
      721 gtccgccaag atcctatgtg caaacgcttg ttagccattc ctagattcag ccctctggtt
      781 agtcaggcaa taaaaagctg gatgggggat ggaaaacagt ttagacgagg aagagatgca
      841 tctgcgactc tgggactggt tccccgacag ttcagcacag gtgggaagca ggttcttctg
      901 ggtataacaa aatttggaga aatgtaatga acacatccca ccccattaag tcaggtgctc
      961 tgtgatggat gaagggaaat actggaaggt gtggagcctc gcagatttcc ggcatcatag
     1021 tgcccaatac aggatgcata aagctccctc agactggaga ctccacaaat gaaatataca
     1081 ccggttggcg ttgatatcgc aaaacatctg attcaggttc atttcatcga cgagcacaca
     1141 ggtgaagtga ttgataagca actacgtcgt caggattttc tgatgttctt ctgtaatcgg
     1201 gaaccctgcc tgattggtat ggaggcctgt agaggctctc agcactgggg gcgtgaactg
     1261 acaaagctgg ggcataaagt ccggttgtta caggcccact ttgttaaggc attcgtcatg
     1321 ggaaataaga atgatgtgat ggatgcccgg gctatctgga tggctgttca gcagccaggt
     1381 aaggacatcg cggttaaaac agaagaacaa cagtcagtgc tggttttgca ccgttcccgc
     1441 agacaattgg ttaagttccg caccatgcag attaattctc tgcacggcac gctcctagaa
     1501 tttggtaaaa ccattcacaa aggtcgggca gcgatggagc gagagctccc tgaagcactg
     1561 gagcggatga aagagagact gccgccgtat ctcattactg tgctggaaaa ccagtacaac
     1621 cggctgaatg agctggactc gctgatagag gatattgaaa aaaagcttac cagcgtggcg
     1681 agacagaatg aaacctgtaa gcggttgctg gatattcccg gcgttgggcc acttattgca
     1741 acggcagcgg tggccaccat gagggaagca tcagcgttta aatcagggcg agagttcgcg
     1801 gcttatgttg gtctggtgcc caaacagact ggctcaggag ggaaaatccg cctgctggag
     1861 ataagcaaac gtggtaatac atatctgcga gccttgttta ttcatagcgc aagggcggca
     1921 gcattactgc ccaaagaacc tggtccgtgg ataaccgaac tgaaaaaacg tcgtccaacc
     1981 agcgtggcaa tcgtcgccat ggcaaacaaa ctagcacgaa cagtatgggc gatagcagct
     2041 catggaagta aatacgacaa gaatcatgtc agtatcagat catattaatc gctgataccg
     2101 ataaatatta acttttaatg aaaggatgaa tactgaaagg taactctggc ggccatagtg
     2161 atgacaacga caggtaagac cgggactcac taaacctgaa tattgtgttg agctaaaagc
     2221 tcgctggaga aatgaggtgt gagtcagcga attacatagg ggctcgcagc ttcatggctg
     2281 caaaaaagcc ggatataaag ctgcaaccta cccgtcactg tcaaggcaat ggatgccttg
     2341 caacgggatg tgttcatata atcaatatgt cagatctatg ctgatacagg gtgccagagc
     2401 ggtattgttc agagcctcag agaaaactga ttcacttcgt gtgtgggtta attgtattcg
     2461 ggaaaagcgt ggcttcaacc aggcagttgt tgctcttgca aataaactcc ttcggatcgc
     2521 ctgggcaatt atcgcccgca atgagatgta tttgcccgca gcaaaacaaa atcaataatc
     2581 tacgtaaaat cagtaggagt aaagcttacc ctgaaattgc aaagaatgca gaatgatgaa
     2641 aaacaggtca gaccgacgtt acgaaaacct gcaagatgag ccaggccagt aaggccgagt
     2701 aagcgataag gtcggagcgc gcgaatgtct catcaaggcc agaagcaata acttcatgaa
     2761 caaaccggat atacgaaagt aaacctgtcc ctatcatagt tgctgtactc ttgcaatacg
     2821 ggggaagacc atctcatcgc ttacgaacaa ttcgtgaaaa cgatgaagaa agactacatc
     2881 gcgtgcatgc cgaaatctaa tgtaagaacg gcattgcata atcttgcagt ggagatcggg
     2941 tattacaatg aaaaccatcc gcatagtgcg ttaggttatc tcccaccaag ggaatatcaa
     3001 cgtcagcgaa taacgtcacc ttagatgcag tagttgtcag gaaatagtga ctcaagggca
     3061 ggcttaccat atacactctt agtggttcga ctgggtatta gaatagctat taacagatta
     3121 gcactgaaca ctgcgtaata gtggttgcac aaacaaaatg tta
//
LOCUS       DAZFLD010000328         3148 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.330, whole genome shotgun sequence.
ACCESSION   DAZFLD010000328 DAZFLD010000000
VERSION     DAZFLD010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3148
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.330"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            183..2492
                     /locus_tag="ACHKBL_004701"
     CDS             183..2492
                     /locus_tag="ACHKBL_004701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerB N-terminal domain-containing protein"
                     /protein_id="HFO5821444.1"
                     /translation="MELWIFIVAIYLIYILFFKKKKTKSAGSYTSAPVKAPPKEWLAN
                     NKKNQSVMQNEDSEDDNLATFTLSGGRNVEYRITATQRQNFSKTTGALARWIFPGEMI
                     TVSGIEVTGGHFYLGQRMKPAGHNSGGYYDGNNEASLVDDTLKIYPTPYIYEDSSLGY
                     WPSFSSLSPEARGAYLSWLASDRCDTSCPIGYVFIYLYGLERRALVDSRDNNISDDEF
                     YSLFKEICRLRSVFNENRSFRNYSSQLLEAMSILRPNENLAAALNDDSEFSNSMQFRL
                     ILAKTVDTGAPVPADLALTWVTNHAEYSLRTPARRCAKEFAALFKRRYTLKYGEGMVV
                     KPNKARLRLDYTPASPSLRGIRLPVPDLPDPGALKGPVQKLMAIADICTGELDAYSRY
                     LGRKGTSANDTAAILLLPSEIVNESAEKILSTFKHWADDAIRSKGGIVSVADYWSHMN
                     ATCPAKINKKEADLMQAFAQKMGYCLAPDPYHHHVKADVDGVLVLFPAGERGRFSPYP
                     EFITAVLTLRLGSVVALIDNSLDQAEQKVLENAINNNASFSDDEKRSLHAYLTWQLHT
                     PANMTGMKSRIELLGAAEKSAVGKVIVSVACSDGRITPAEIKQLEKIYTSLGLDPSSV
                     SSNIHQHSATETALVSSVPANQSTAGFTLDANVLARHESATDDVRKLLNSIFTEEEPE
                     QPESAPASLTGEGGLDSAHSQLYRRLLEKEQWSRKEATELCSNWNLMLGGALEVINDW
                     SYAVVDAPVLEDADDHIWIDLEIAKELEG"
     gene            2496..2897
                     /locus_tag="ACHKBL_004702"
                     /pseudo
     CDS             2496..2897
                     /locus_tag="ACHKBL_004702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000117530.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BREX system ATP-binding domain-containing
                     protein"
     gene            complement(2868..3076)
                     /locus_tag="ACHKBL_004703"
                     /pseudo
     CDS             complement(2868..3076)
                     /locus_tag="ACHKBL_004703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869530.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          866 a          706 c          737 g          839 t
ORIGIN      
        1 tactgatctc ctctggttac agataacgac ttcagccctc tgaaagagaa aagcggaagc
       61 tcaggaaggt tgtgatttgc ttttaacctt tttaatttag aagtattatt ttgagattaa
      121 tccacttccc atcgtcatcc tttttggtgt gcgtgaaaag ctatttttaa ggttactgcc
      181 tcatggagtt atggatattt atagtagcta tttatttgat ttatattctt tttttcaaaa
      241 agaagaaaac taagtccgcg ggctcttata cttccgctcc tgtgaaagca ccacctaaag
      301 aatggcttgc taacaacaaa aaaaatcagt ctgtaatgca aaacgaggac agcgaagatg
      361 acaatctggc tacgtttaca ctaagtggcg ggcggaatgt cgaatacagg ataacagcca
      421 ctcaacgcca gaatttctca aaaactactg gtgctttggc tcgttggatt tttccgggag
      481 agatgatcac agtcagtggg atagaggtta ccggcggtca cttctatctt gggcagcgta
      541 tgaaacctgc cgggcataac tcaggcggtt attatgatgg caataacgag gcatcgctag
      601 ttgacgacac gcttaaaata taccccacac cttacattta cgaagatagt tcattagggt
      661 attggcccag tttttcctca ctttcacctg aagctcgtgg tgcgtacctg agttggcttg
      721 ccagcgacag atgcgacact tcctgcccaa ttggttacgt tttcatttat ctgtatggtc
      781 tggaaagaag ggcgcttgtt gacagcaggg acaacaatat atccgacgat gagttctatt
      841 ctcttttcaa agagatctgc cgtctcagat cggttttcaa tgaaaaccga tctttccgga
      901 attactcatc ccagttactt gaagcgatgt ccattcttcg tccgaacgaa aatcttgcag
      961 cagctctcaa cgatgattcg gagttcagca atagcatgca gttcaggtta atcctggcaa
     1021 agactgttga tacaggagcc ccggttccag cggatctggc tctgacgtgg gtgacaaacc
     1081 atgctgaata ctcattacga acccccgccc gccgctgtgc gaaagaattt gctgcactat
     1141 ttaaacggcg ttacaccctt aaatacggtg aagggatggt agtgaagccg aataaagcgc
     1201 gtctgcgttt agattacacg cctgcaagtc ccagtttgcg aggtattcgg cttcctgttc
     1261 ccgacttgcc tgatccaggt gcattgaaag gcccagttca gaagcttatg gccattgccg
     1321 atatatgcac aggtgaactg gatgcttaca gtcgttacct tggtagaaaa ggtacttcag
     1381 caaatgatac cgctgcaatt ttgttgctgc cttcagaaat cgttaacgaa agcgcagaaa
     1441 aaatattaag tacattcaaa cactgggctg atgacgcaat ccgcagtaag ggtgggatag
     1501 tttcagttgc tgactactgg tcacatatga atgccacctg tccggcgaag atcaataaaa
     1561 aagaggccga cttaatgcaa gcctttgcac agaaaatggg atactgtctg gctcctgacc
     1621 cctatcacca ccacgtaaag gcagatgtcg atggtgtact tgtcttattc cctgctggtg
     1681 aaagaggacg tttctcacct tatcctgaat ttatcacagc tgtattgacg ctccgattag
     1741 gttcggtagt cgccttgatt gacaattctc tcgatcaagc cgaacagaaa gtgcttgaga
     1801 atgccatcaa caacaatgcc agcttcagcg atgatgaaaa gcgctctctg cacgcatatt
     1861 taacgtggca gcttcatact ccggcgaata tgacgggaat gaaaagccgg attgaattac
     1921 tcggtgcagc ggaaaaatca gctgtgggta aggttatcgt tagtgtcgct tgctctgacg
     1981 gcagaattac gcctgccgaa atcaaacagc ttgagaagat ttatacaagc ctgggactgg
     2041 atccctcatc cgtatcaagc aatatccatc agcattccgc aactgaaact gctctcgttt
     2101 catctgtacc agctaatcag tcgacggcag ggtttacgct ggatgccaac gtacttgccc
     2161 gccacgaatc tgccactgat gatgttcgta aattactgaa ctcaattttc actgaagaag
     2221 aaccagaaca accagaaagc gctccagcct cattaaccgg ggaaggggga cttgactccg
     2281 ctcatagcca actttatcgc aggttgctgg agaaagaaca atggtccaga aaagaggcga
     2341 cagagttgtg tagtaactgg aacctgatgc tcggcggtgc gcttgaggtg attaacgatt
     2401 ggtcctatgc ggtggttgat gcaccagtgc tggaagatgc tgatgatcac atttggattg
     2461 acctggaaat agccaaagaa ttagagggtt agtgaatgtc tgtaacacgt ataagagtga
     2521 aagagcgcga cgctatcatt cagtcactta agtcgggagt aacgccaaga ataggtattc
     2581 agcatatcca ggttggccgc gtgaatgaga taaccgccct ccatcaggat attgaacgga
     2641 ttgctgatgg cggggcaagc ttcagactta tcattggtga gtacggttcc gggaaaacat
     2701 tctttttaag tgttgtacgg tctattgcgt tagagaagaa gctggtcacg gtcagtgcag
     2761 acctttctcc cgacagacgt atccactcat cgggaggtca ggctcgtaat ctctactcag
     2821 agcttatgaa aaatatgtcc accagaaaca aaccagatgg caatgcatta cttagcgttg
     2881 tggaaaggtt tgttacagtc aaggcagtca ccaaactcga taataaagcg gctcatttcc
     2941 atacgtctgt ccttaagggg cattgttcat ttctgtgacc aagccgcacc acctttttta
     3001 ctgattcgtc tgtcgggaac actttgcgtt tcttgatgac ataacggatc acactattca
     3061 acgactctat ggcattttgc acatacttcc atagcaactg tcatcggtag ctactgaacc
     3121 agcaagttaa gtaagttact acgtgaaa
//
LOCUS       DAZFLD010000329         3146 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.331, whole genome shotgun sequence.
ACCESSION   DAZFLD010000329 DAZFLD010000000
VERSION     DAZFLD010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3146
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.331"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            7..1296
                     /locus_tag="ACHKBL_004704"
     CDS             7..1296
                     /locus_tag="ACHKBL_004704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014728563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VRR-NUC domain-containing protein"
                     /protein_id="HFO5821445.1"
                     /translation="MEMPDLPETPTSDIPTGANNQPWDCTTTGVEMVEKQSRLPTTEN
                     RQYLHIKVEYAMRFPQLSAQIRRKGKTYQMSLKQTVMSGLIRVDEIARNYCWYYKAEV
                     AFDMRTTPPRPFLSSTLLEKDGAGRRHTTNPFPVPGRGIYRRPDVIIVKNKEDRWPGL
                     AGPDTEGNMHSDNLARLVEVKFPGDRLYEDQYEEYMQIVGGIGHEDRMTILEIHDCRP
                     EEHQWVDQVINVTMKAWQTSGAKYWPLALYPPVFMPRPPSTPVAARIEPWTYAGKAVS
                     DLSEGVVRGVAEGWNALSKETQLVLNSAASWLNDSGEWVYRQGSEVWVWASQTGKSVK
                     SWTNTQIKAAWTEIQHATDMTLDMLKQVDWVQVLTSVAKTVGVILVAVGVGGLVVTLG
                     IPEAIIGAFLLIVRLAISAWGTLATILGTGTAVLAVG"
     gene            1309..2523
                     /locus_tag="ACHKBL_004705"
     CDS             1309..2523
                     /locus_tag="ACHKBL_004705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3396 domain-containing protein"
                     /protein_id="HFO5821446.1"
                     /translation="MELHDFELNYIEKWLPEASVKYKDGSPAVNPGLIITVFFKDGHT
                     PEVRRRMVECVDRFYGEFKPWLKKTITKNWVGITDNNYAKKRQEILDSTPEEIFSWYL
                     SSAEKDFLAPDYSILIMGKRIFHNDNNRSVIKLTLPLSLLKETDGKQRYEAWLMWLCD
                     TFSVESGYAGLSFALPYARERMFPCEFALAQRFSGVMVDSLGTLEGGEAVEGLKGACW
                     YTILGTPWLEKLGGAERVKHRLAKTPEISLLPYNNGVVLKAGELPPPLGEMKTEGLSP
                     LLVKVNQVIRSIRYDGRNGLHFYSEYEGLQFEEASSMAWFARFDEASALLDKEEMGKS
                     CAPERIKCWTDEIAPHAGQWAAMVNGTTEYIQTREGQKMPPFEDKHGKQHRACWKLLK
                     RDDNGSVYTLPE"
     gene            2537..2890
                     /locus_tag="ACHKBL_004706"
     CDS             2537..2890
                     /locus_tag="ACHKBL_004706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2345 domain-containing protein"
                     /protein_id="HFO5821447.1"
                     /translation="MTVQAGERLGLFARTGQLSLKSGEGAVDVQAQNGSMRLFAEKKL
                     TLSSASDISFAGKKRITLIGGGSYLRLEAGKIEYGTMATYLRRTKRTMVAAPATMPLP
                     IPLLLDLYPPLNGQI"
BASE COUNT          807 a          719 c          898 g          722 t
ORIGIN      
        1 gccataatgg aaatgcccga tttacccgag acaccgacat ctgatattcc caccggggcg
       61 aacaaccagc cgtgggactg cactacgacg ggtgtcgaaa tggtcgaaaa gcagtcgcgt
      121 ctgccaacca cggaaaaccg tcagtacctg catataaagg tggaatacgc catgcgtttt
      181 ccgcaactgt cggcacaaat ccgccggaaa ggtaaaactt accagatgag ccttaagcag
      241 accgttatga gtggtcttat tcgtgtggat gaaatcgccc gcaattactg ctggtattat
      301 aaggcggagg tggcgtttga tatgcgcacc acgccaccac gcccttttct gagcagtacg
      361 ctgctggaaa aagacggtgc tggccgtcgc cacacgacca atcctttccc tgtgcccggc
      421 aggggcattt accgccgccc ggacgtgatt attgttaaaa ataaggagga ccgctggccc
      481 ggactggcgg ggccggatac cgaaggcaat atgcacagcg ataacctggc ccgcctggtg
      541 gaggtgaagt ttccgggaga taggctgtat gaagaccaat atgaagagta tatgcagatt
      601 gtcggtggaa ttggtcatga ggatcgcatg accattctgg agatccacga ctgtcgcccg
      661 gaagagcacc agtgggttga tcaggtgatc aacgtgacga tgaaagcctg gcagaccagc
      721 ggcgcgaaat actggccgct ggctctctac ccgccggtct ttatgccccg tccgccatca
      781 acccccgttg ctgccagaat agagccctgg acttacgccg gtaaagcagt atctgatctc
      841 agtgagggtg tggtacgtgg tgtggctgaa ggctggaatg ccctgtcaaa agagacgcaa
      901 ctggtcctga attcagcagc cagctggctt aacgacagcg gtgagtgggt ttaccggcag
      961 ggaagtgagg tctgggtctg ggccagccaa accggtaagt cggtgaaatc ctggaccaac
     1021 acccagatta aggccgcatg gacagaaata cagcacgcca ccgatatgac gcttgatatg
     1081 ctgaaacagg tggactgggt acaggtttta accagtgtcg cgaaaacggt cggcgttatt
     1141 ctggttgctg tgggtgtcgg cggactggtc gtaacgctgg gtatcccgga agccattatt
     1201 ggcgcgtttt tactgattgt tcgtctggca ataagcgcct ggggaacact ggcgacgatt
     1261 ctcggtacgg gtacggcggt actggcagtt ggttaaacag gagcaggtat ggaattgcat
     1321 gattttgagc tgaactacat tgagaaatgg ctgccggaag cctccgtgaa atacaaagac
     1381 ggcagtccgg cagtcaatcc cggtctgatt atcaccgtct tttttaagga cggacatacg
     1441 ccagaagtcc gccgcaggat ggtggagtgc gtggatcgct tctatgggga gtttaaaccc
     1501 tggctgaaaa aaacgatcac caaaaactgg gttggcatca ctgataataa ctacgccaaa
     1561 aagcggcagg agatcctcga ttcaacgcct gaagagattt tttcctggta tctgagtagc
     1621 gcagagaagg attttttagc ccccgattac agtattttaa ttatggggaa aagaattttt
     1681 cataatgaca ataatcgttc agtcattaag ttaacactcc cgttgtcact gctgaaagaa
     1741 actgatggaa agcagcgtta tgaagcctgg ctgatgtggt tgtgtgacac cttttcggtg
     1801 gaaagtggtt atgcagggtt gtcgtttgca cttccttatg cccgtgagcg aatgttcccc
     1861 tgtgaattcg ccctggcgca gcgtttttcc ggtgtgatgg tggattctct tgggacgctt
     1921 gaaggcggtg aagccgttga ggggctgaaa ggtgcctgct ggtacaccat tctcggtacg
     1981 ccgtggctgg aaaaactggg gggggcagag agagtaaaac atcgtttagc taaaacgcct
     2041 gaaattagcc tgctacccta taacaatggt gtcgtactta aagccggtga actccccccg
     2101 ccactgggag agatgaaaac agaaggttta tcgccgctgc tggtgaaggt gaaccaggtc
     2161 attcgttcca ttcgctatga tggacgcaat ggtctgcatt tttactcaga atatgaagga
     2221 cttcagtttg aagaagcgtc ttcaatggca tggtttgccc gttttgacga agccagtgcc
     2281 ttgctcgata aagaagaaat ggggaaatct tgcgcccccg aacgcattaa atgctggacc
     2341 gatgaaatag cgccccatgc cggacaatgg gccgcgatgg ttaatggcac caccgagtac
     2401 atccagaccc gcgaagggca gaaaatgccc ccgtttgagg ataagcacgg taagcagcac
     2461 agggcttgct ggaaactact gaaacgagat gacaatggta gcgtttatac actacctgag
     2521 taaggtcgtg tgctgaatga ccgtgcaggc cggagagagg ctggggcttt ttgcccgcac
     2581 cggtcaactt agcctgaaat ccggggaagg ggcagtggat gttcaggcgc aaaacggcag
     2641 catgcggttg tttgctgaga agaagctgac gctgagctca gcgagtgaca tctcctttgc
     2701 cgggaaaaaa cgcatcacgc tgattggcgg gggaagttac ctacggctgg aggcggggaa
     2761 aatagagtac ggtacgatgg cgacgtatct gcggcggacg aaacggacga tggtagcagc
     2821 cccggcaaca atgccgctcc ccataccgct tctcctggac ctgtatccac cacttaatgg
     2881 ccaaatttga attgaatgtc tcctcaatgc catcaaagtc aacgatgcta tcgttcaggg
     2941 agcgtagtca atggatgtca caaaaatgat gcaggtgcta caggatttgt ttttatgctg
     3001 ctaaatgtac agcgacttcg tgatatgggg ttgttggtgt tttttataat aatgcttttt
     3061 acggaatcaa aaaaacagac aacatattaa ttatttgaga aagcagcgtc aaaaaggaaa
     3121 agttaaaatg tcgaatcgat ttttta
//
LOCUS       DAZFLD010000330         3142 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.332, whole genome shotgun sequence.
ACCESSION   DAZFLD010000330 DAZFLD010000000
VERSION     DAZFLD010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3142
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.332"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            39..>3142
                     /locus_tag="ACHKBL_004707"
     CDS             39..>3142
                     /locus_tag="ACHKBL_004707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5821448.1"
                     /translation="MAFSDEQRPEAQLGNQNRSSLNIKQPGENYWQDFFSNPQNAIDH
                     STSFSLGDVLPTMGKGFAQSVRGTGEMVRGLGDGFVNNPVKTLAGLSSFMTDVYMPPS
                     STQKELGAPPSVGDAIKNIPDGTGKNAVTDTVGKGLKATGKAVSDGAEASDEWLTDQM
                     SPGAVRALNTPMSEGYDDSAVLLTKGVNLIGALVPDLVAGGVVRKVGQVALKRTLTAG
                     LEKKYIAAGVQPERATALAAEAVDKKMTDFFHAGLITHSTASAQGQSAMSAADAVLNA
                     DYAELAQSPKFQQTFLSIDADPQHAQLTDRQKMDLAKERVADEVRAQLATDPELLAVN
                     AMAAKLGDAQLFNLVTRGTAKTVKSGIVRNATEQGVINGAQNAYSRYQENTALRETAG
                     MDVSPWEGVADATIEGAAFGAAMGAPFGAVAGYRGRRQAAEEAAMRDAETVQQDDAAP
                     QPESVDPVAQQRESMQGMNREQLLEQYADADMAHEGDTSAVHRREAASQLLNELDEQA
                     KRQAVMDELKAKPRPELLEEYRKLSLKEGRTDTEEQQFQAIREVIRPQQEVTPEAQSQ
                     PENAEDGNGSIYPTVRFRDPNEVRIEINGNGASRPAERIEKVRPDNRYFTDEKSAMGS
                     DVFRNAAATGLKPSVVKKGENQYAVEMDNPAFSEDVATETINTLADRERIADADPMEQ
                     PAFMRDPRFRGFTGDDTEVQARLARGNAPTAEELVRSQMAEGDAGPTAQELTERPRLP
                     APGDIHPGQGYPLPGEVARTPDENQAGRGGRFTTTGEVKGQSFQKGQAPAPENAAGRQ
                     GEILEGETVRRGLPSPDAQNATAPVREGLPAPDIARNVRMPQPESLPRTVRDSLPELA
                     QQAEVRRQAGGNRDIPQPETIAPESETTVSTDREATVRGGEVRGKKIEDFGEEIKGAA
                     KHRYAQLAETLGKTLEDRDYATQPLSKLFPKPDYAKLANEGADADTLAMIALYRSDIP
                     AKTKHNTAGWGESIKKVRHSVSEMLNGTVSAKRLAEWMEGRMPSRYADTWQLLRTLPP
                     SQMD"
BASE COUNT          804 a          781 c          966 g          591 t
ORIGIN      
        1 cccgaccatc gtgtcgggct ttttttactg gagtctgtat ggctttttca gatgagcagc
       61 gccctgaagc gcaattgggt aaccagaacc gcagcagcct gaacattaag cagccgggag
      121 aaaattactg gcaggatttt ttcagcaacc cgcagaatgc cattgatcac agcacatcgt
      181 tcagcctcgg tgatgtattg ccaacaatgg gtaaaggttt cgcccagtcc gtccggggaa
      241 caggggagat ggtccgtgga ctcggtgatg gatttgtaaa taatcctgta aaaacgcttg
      301 ccgggttgtc gtctttcatg acggatgttt acatgccgcc ttcctccacg caaaaggaac
      361 tgggcgcgcc accttctgtt ggagatgcga ttaaaaacat tcccgatgga acaggtaaaa
      421 acgcggtcac tgatactgtc ggtaaaggtc tgaaggcaac cggtaaggct gtcagtgatg
      481 gtgctgaagc ctcggatgaa tggctgacag atcagatgtc gccgggtgct gttcgtgcgc
      541 tgaacacgcc aatgagtgaa ggctatgatg attctgcggt cttgctgacg aagggtgtaa
      601 acctgattgg tgcgcttgta cctgatctgg ttgctggcgg tgtggttaga aaggtgggac
      661 aggtcgcact gaaaagaacg ctgaccgccg ggctggagaa aaaatacatc gcggcaggag
      721 tgcagccgga aagagccacg gcactggcag cagaagctgt cgataaaaaa atgacggatt
      781 tcttccatgc gggcctgatc acccattcca cagccagtgc acaggggcag agtgcaatgt
      841 cggcagcaga tgctgttctt aatgcggatt acgctgagct ggcgcagtca ccgaaatttc
      901 agcagacgtt tttgtcaatt gatgcagatc cacagcacgc acagcttact gatcgccaga
      961 aaatggacct ggcaaaagag cgtgttgccg atgaggtgcg cgcgcagctg gcaaccgatc
     1021 ctgaattgct ggctgtgaat gccatggcgg caaaactggg tgacgcacaa ctgtttaatc
     1081 tggtgacacg aggcacagcg aagaccgtta aaagcggcat tgtcagaaat gccacggaac
     1141 agggcgtaat taacggggca cagaacgcct attcgcgcta tcaggaaaac acggcattgc
     1201 gtgagaccgc cggaatggat gtgtcaccgt gggagggcgt ggctgacgca acgatcgaag
     1261 gtgcagcctt tggtgctgcg atgggggctc cattcggtgc ggttgccgga tatcgtggca
     1321 gacgtcaggc cgcagaagaa gcagccatgc gtgatgctga aaccgtgcag caggacgacg
     1381 cagccccgca accagaatct gttgatccgg tggcgcagca gcgtgaatcc atgcagggca
     1441 tgaatcgcga gcagcttctg gagcagtatg ctgatgcgga tatggcacat gagggagaca
     1501 cgtctgccgt tcatcgccgg gaagccgcca gccagctgtt gaatgaactg gacgaacagg
     1561 cgaagcgaca ggcggtgatg gatgagctga aggcgaagcc gcgccctgaa ctgcttgagg
     1621 aataccgaaa actcagcctg aaggaagggc gtactgatac tgaagagcag cagtttcagg
     1681 caatacgtga agtcattcgc ccacaacagg aagtgacgcc tgaagcacag tcacagcctg
     1741 aaaatgcgga ggatggtaac gggagcattt acccgacggt gcggttccgg gacccgaatg
     1801 aagtccgcat tgaaattaac gggaatggtg cgtccagacc agcggaacgc attgaaaagg
     1861 tgcgcccgga caaccgttat ttcacggatg agaaaagcgc catggggagt gatgttttcc
     1921 gtaatgccgc cgccaccggc ctgaaaccgt ccgtagtgaa gaaaggcgag aatcagtatg
     1981 ccgttgaaat ggataatcct gcgttctctg aagatgtggc aacggaaacc attaacaccc
     2041 tggctgacag agagcgtatt gctgatgctg acccgatgga gcagcccgcg ttcatgcgtg
     2101 acccgcgatt ccgtggtttc acgggggatg atacggaggt acaggcccgc cttgcccgtg
     2161 gcaacgcgcc gacggcggag gagcttgtac gttcacagat ggctgaaggt gatgccggtc
     2221 cgacagcaca ggagttaact gagcgtccgc gtctgcccgc ccccggcgat attcatcccg
     2281 gacagggata tccgttaccg ggagaggtgg cgcgtacgcc ggatgagaat caggccggac
     2341 gtggtggtcg ttttaccaca accggtgagg ttaagggcca gagtttccag aaaggacaag
     2401 ctccggcacc ggaaaacgcc gctggtcgcc agggggaaat actggagggc gaaacagttc
     2461 gtcgtggtct gccgtcaccg gatgcgcaga acgcgacagc accggtacgt gaagggctac
     2521 cggctcctga cattgcgcgt aatgttcgta tgcctcagcc tgaatcactt ccccgcactg
     2581 tacgggactc actgcctgag cttgcacagc aggcagaagt acgccgacag gccggaggaa
     2641 atcgtgacat cccgcagcct gagacaatcg cacctgaatc tgaaacaact gtctctactg
     2701 acagggaagc taccgtgcgc ggaggtgaag tcaggggcaa aaaaattgaa gactttggcg
     2761 aggaaattaa aggggcagcc aaacaccgtt atgcacagct tgctgaaaca ctgggtaaaa
     2821 cgctggaaga cagggattat gccacgcagc cgctgagcaa actgttcccg aaaccggact
     2881 acgcaaaact ggcgaacgaa ggtgccgatg ctgataccct ggcaatgata gcgctgtatc
     2941 gtagcgatat tccggcgaag acgaaacaca atacggcagg ctggggggag agcataaaaa
     3001 aagtacgaca cagtgtatcg gaaatgctga acggaacggt cagcgcgaaa cgcctcgcag
     3061 aatggatgga aggcagaatg ccctcccgtt acgcggacac ctggcaactg ttacgcactc
     3121 tgccaccctc acagatggac ag
//
LOCUS       DAZFLD010000331         3134 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.333, whole genome shotgun sequence.
ACCESSION   DAZFLD010000331 DAZFLD010000000
VERSION     DAZFLD010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3134)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3134)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3134
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.333"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(14..324)
                     /locus_tag="ACHKBL_004708"
                     /pseudo
     CDS             complement(14..324)
                     /locus_tag="ACHKBL_004708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(708..1517)
                     /gene="iolB"
                     /locus_tag="ACHKBL_004709"
     CDS             complement(708..1517)
                     /gene="iolB"
                     /locus_tag="ACHKBL_004709"
                     /EC_number="5.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-deoxy-glucuronate isomerase"
                     /protein_id="HFO5821449.1"
                     /translation="MTNLLSTCTTRAGNIQHISPQNAGWEYVGFDVWLLKAGESLTLP
                     SDDRERCLVLVAGLASVKAADHFFYRIGQRMSPFERIPAYSVYLPHHTEAKVTAETDL
                     ELAVCSAPGFGELPVRLISPQDIGVEHRGKGRNQRLVHNILPDTQPADSLLVVEVYTR
                     EGDTSSWPAHKHDTAVEGQETYLEETYYHRFDPPQGFSLQRVYTADRSLDECMAVYNR
                     DVVKVPKGYHPVATIAGYDNYYLNVMAGPLRKWQFTWEADHAWINSSEYPR"
     gene            complement(1542..3047)
                     /gene="iolA"
                     /locus_tag="ACHKBL_004710"
     CDS             complement(1542..3047)
                     /gene="iolA"
                     /locus_tag="ACHKBL_004710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate-semialdehyde dehydrogenase IolA"
                     /protein_id="HFO5821450.1"
                     /translation="METVGNFIQGKTRLSNSGETLPVTNPATGEVIRLVTQSTRQDML
                     EAIQSAHEAFPAWSKMAPLRRARILFEFKVLLEKHRDELAALIVSEHGKVWSDALGEL
                     TRGIEVVEFACGIPHLIKGEFSFNVGTGVDSFSLMQPLGVVAGITPFNFPAMVPMWMF
                     PIALACGNTFVLKPPALVPSASLRMAQLLQEAGLPDGVFNVVHCGNDAASLLTSDPRI
                     QAVSFVGSSSVAEHIYTTASAHGKRVQAFGAAKNHAIVMPDADLDATVNAIMGGAFGS
                     AGERCMALPVVVAVGDETANRLIERLKPLIAALRIGPGELREKDENEMGPVVSRMHQQ
                     KVLAYIDKGVSEGATLVMDGRNYCVKGYPEGFYVGGTLFDNVTPEMTIWREEIFGPVL
                     GIVRVTDYATAIRTVNSHEFGNGSVIFTSNGHYAREFAQSVEAGMVGINVPVPVPMAF
                     HSFGGWKRSVFGALNVHGPDGVRFYTRMKTVTARWPNGQQIASEFSMPTLG"
BASE COUNT          821 a          787 c          727 g          799 t
ORIGIN      
        1 aacaaccatt cggctatatt atctttttca tgttggtgct tttattctgt gaggtgtgct
       61 ggcatggtgg acacaaccat ttgttctttt tagctgccat ttgataatat tttcctgaag
      121 ctttccgggg atatattccc cagacgggag tggcggcgct gacggttgta gaatatctca
      181 atgtataccc agtattactg agatgacttc attccggcta ctaaaatggt agtggttcag
      241 actttcattc ttcagagttc cccaaaagct ttccataggg gttttgtcgt aacagttccc
      301 tttacgagac attgatgttt tcagagctaa ctgcgataga ctccttcaac ttcaacttca
      361 acttcaactt caacttcaac ttcaacttca actgagaaac ggatgatttc acgatagact
      421 gcccaacgta ttgagcccgt taggaagcca gagtaacaag ccgccgtgta cggcatttct
      481 ctcctaattc ggcagaacca aaaatattgt cagctcagga gtaagaatca gaaaaaacca
      541 taacagaaca ccagaaacag gatattatca gacagagaaa aaattcaaaa aaatccaccg
      601 attctgttgc ctgtggattt gtgtataaga ggcagaaccc tgttggggac tgtgcacaaa
      661 aaagccagct gcagaggaca actggcaaag gaatatttaa cgtattgtta acgcggatac
      721 tctgacgaat taatccatgc gtgatcagct tcccatgtga attgccattt gcgtaatggc
      781 ccggccatca cattcagata atagttatcg taacctgcga tagtggccac cggatgatag
      841 cctttcggga cttttaccac atcgcggtta tatactgcca tacattcgtc cagactgcga
      901 tcggcggtgt agactcgctg taagctgaat ccctggggtg gatcgaaacg gtggtagtag
      961 gtttcttcca gataggtttc ctgcccttca actgctgtgt catgcttatg ggcaggccag
     1021 gaacttgtat cgccttccct tgtatatact tccactacca gcagactgtc agcaggttga
     1081 gtgtctggga ggatattatg aaccaagcgc tgattgcgcc cttttcccct atgttccacc
     1141 ccaatatctt gtggtgagat cagtcttacc ggaagttcac cgaatccagg agcactgcag
     1201 acagccagtt ccagatcagt ttcggcagtg acttttgctt ctgtatggtg cgggaggtaa
     1261 acagaataag ccggaattcg ctcaaaagga ctcatgcgct ggccaatccg ataaaaaaag
     1321 tggtcagcgg cctttacgct ggcaagtccg gcaaccagaa ccaggcaacg ttcgcgatca
     1381 tcggatggca aggttaaaga ttcgccggct ttcaacagcc agacatcaaa accgacatac
     1441 tcccagcctg cgttttgcgg agaaatatgt tgaatatttc cagccctggt ggtgcaggta
     1501 ctcagcaagt tggtcataat cttctccttc ctgaacgttt attaacctag cgtaggcata
     1561 ctgaactcgg atgcgatctg ctgaccgttc ggccagcgtg cggtaacggt tttcatccgg
     1621 gtataaaaac gcaccccatc cggtccgtgg acattcagcg ccccaaacac cgagcgtttc
     1681 cagccgccga aagagtgaaa cgccattgga accggtaccg gaacattgat accgaccatc
     1741 ccggcttcaa ccgattgagc aaattcgcgg gcataatggc cgttggaggt gaagatcacg
     1801 ctaccattgc caaattcatg agagtttacc gtacgaatgg cggtggcata atctgttacg
     1861 cgtacgatgc cgagcacggg accaaaaatt tcctcacgcc agatagtcat ttccggcgtg
     1921 acgttatcaa acaaggtacc gccgacataa aatccttcag gatagccctt cacgcaataa
     1981 tttcgcccat ccatcaccag tgttgctcct tcgctaacgc ctttgtctat atatgcaagg
     2041 actttttgtt gatgcatacg tgaaacaact ggccccattt cattttcgtc tttttctcgt
     2101 aactcaccgg ggccaatacg cagcgctgca ataagtggtt ttaggcgttc aataagacgg
     2161 ttggctgttt catcaccgac agcgaccacc acaggtaatg ccatacaacg ttcgcctgcg
     2221 gaaccaaaag ccccacccat aatagcgtta acggtagcat caaggtcagc atccggcatg
     2281 acaatagcgt ggttttttgc ggcaccgaaa gcttggactc gcttaccgtg tgcactggcg
     2341 gtggtgtaga tatgctcagc gacggaagaa gatccgacaa aactgacggc ctgaatgcgt
     2401 ggatcactgg tcaacaggct ggctgcgtca ttgccacagt gaactacatt gaatacacca
     2461 tccggtagtc cggcctcctg tagtagttgc gccatacgta acgatgcgga gggcaccagc
     2521 gctggaggct tcaggacgaa ggtattgccg caggcgagag cgatagggaa catccacatt
     2581 ggcaccattg cagggaagtt aaagggtgtg atgccagcta caacaccaag tggttgcatt
     2641 aatgaaaagc tgtcaacgcc tgtgccaaca ttaaaagaga actctccttt aatgagatgc
     2701 ggaatgccgc aggcgaattc aacaacttcg attccgcgag tcagttcacc aagcgcgtcg
     2761 gaccatactt tgccatgttc actgacgatc agagccgcca gttcatctcg gtgtttttcc
     2821 agcaacactt taaattcaaa aaggattctt gcacggcgca acggtgccat tttactccat
     2881 gcaggaaaag cttcatgagc actctgaatc gcctccagca tatcttgccg ggtgctctgg
     2941 gtaaccaggc gaatcacttc tccggttgca gggttagtca ccggtagtgt ttcaccactg
     3001 ttgctcagtc tcgttttacc ctgaataaaa tttccaactg tttccatcac tgtttacctt
     3061 cttgaatcaa tgttaacgac gttccgactg agtgttcaca ggcaaagcct gatcatctac
     3121 agtctaaaat caca
//
LOCUS       DAZFLD010000332         3113 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.334, whole genome shotgun sequence.
ACCESSION   DAZFLD010000332 DAZFLD010000000
VERSION     DAZFLD010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3113)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3113
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.334"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..1699)
                     /locus_tag="ACHKBL_004711"
     CDS             complement(<1..1699)
                     /locus_tag="ACHKBL_004711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5821451.1"
                     /translation="MMNIRYPVRKADGRDYKSYDELLTDIRKNAHGWWLLGVNRYWHG
                     GIHVGRSSSPASVLNQETPEKSVPLQFMMDGEVVAWRVNRDYAAIECYQERPLRQSGT
                     FVLVKSVYKPDEQDESSWLTLYQLYMHIAPLSEFPKRPLYRVTQKGHGVRMRKYSRHD
                     DSREIAPDVLANKHGHPRTLTQGDTLAVLQQKSFLLKQRPESFALVQRLQDGKPAGEL
                     FWVSMRPEYLEPDGQCYVCLPDWMHSALNHGVFDDVVVPPVPLKVMVKAGDAVGFLGA
                     QDLADEDNYPQIITTDYKAHIELLSLDEHVPDVVANMKGIKTGKQFIKLKLKRPLYLR
                     NGEGEESTFEQMSAITRIDAGKIIPRDATYPFTDKTGITYFQIRPHTWMHQNDVEQLS
                     QHDLAGLNFHCIEAEHTTDFTRTLDERWLIDALKSISSHFDGEKGPQFAQAKMFYDSL
                     IHNAENRRAPNPYPDKSLDQYLFGALHTNQMNIPEYARRLIVKHDSCWHSTRDDTRWS
                     SVCKSRDESPVVKMANGGFLDATHWMDKVPPFASQRSVWHFHPLEFPEILRSAEFPRT
                     "
     gene            complement(2040..2254)
                     /locus_tag="ACHKBL_004712"
                     /pseudo
     CDS             complement(2040..2254)
                     /locus_tag="ACHKBL_004712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742560.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            complement(2260..2946)
                     /locus_tag="ACHKBL_004713"
     CDS             complement(2260..2946)
                     /locus_tag="ACHKBL_004713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HFO5821452.1"
                     /translation="MATMTDHQKEFISLFNRTACYHKRHKVFEDFVSCSVIALENRLQ
                     FSEAREQKYLRIVSGYEKQDVIHIAHLLAHVIEGLEQGFCDFLGSVFMQLELGDKYRS
                     QFFTPWSVASMMAQMQLGNVKALFESKPFITLSEPACGAGSMILAMVDTLNRSGYPAY
                     HRMWVSATDIDPLAAGMAYIQLSLCGVAGEVVIGNSLCNERRRVLLTPGHYLGNWDHR
                     LRHLQEQNVA"
BASE COUNT          756 a          812 c          722 g          823 t
ORIGIN      
        1 gagttctggg aaactctgca cttcgtaata tttccggaaa ctcaagcgga tgaaaatgcc
       61 atacagaccg ctgactggcg aacggaggca ccttatccat ccagtgtgtt gcatccagaa
      121 atcccccgtt agccatcttc acaaccgggc tttcatccct ggacttacat accgatgacc
      181 agcgtgtatc atcgcgggta ctgtgccagc agctgtcgtg tttcactatc aggcgtcgcg
      241 catattccgg gatgttcatc tgattggtat gcagcgcacc aaacaaatac tggtccagag
      301 atttatcggg atacgggttt ggcgctcttc tgttttcagc gttgtggatc aggctgtcat
      361 aaaacatttt tgcctgcgcg aactgcggtc ctttttcacc gtcaaagtgc gagctgatgc
      421 ttttcagggc atcaatgagc cagcgttcat ccagcgtgcg ggtaaaatct gttgtgtgtt
      481 cagcttcaat acagtgaaaa tttagccctg ctaaatcgtg ctgactgagc tgctccacat
      541 cgttctggtg catccacgta tgtgggcgaa tctgaaagta tgtgatcccg gttttatccg
      601 taaacgggta cgtggcatca cgtgggataa tttttccggc atcaattcgg gttatggcgc
      661 tcatttgttc aaatgtactt tcttcaccct ctccatttcg caggtacagc gggcgtttca
      721 gcttcagctt aatgaactgt ttgcctgttt taatcccttt catgtttgcc accacatctg
      781 gcacatgctc atcgagactc agtagctcga tatgggcttt atagtccgtg gtaataattt
      841 gcggatagtt atcttcgtca gccagatcct gagcgccgag gaatccgacc gcatcccctg
      901 ctttcaccat cactttcagt ggcacaggcg gcaccaccac atcatcaaac acgccgtgat
      961 tcagcgcact gtgcatccag tcaggcaggc acacgtaaca ttggccatca ggttccagat
     1021 attccggacg catcgagacc cagaaaagtt ctccggcggg tttaccgtcc tgaagacgtt
     1081 gcaccagagc aaacgactcc ggccgctgtt tcagtaaaaa cgatttttgt tgcagtacgg
     1141 caagcgtgtc accctgcgtc agtgttctgg ggtggccgtg tttgtttgcc agtacgtccg
     1201 gcgcgatttc acggctgtca tcatggcggg aatatttacg catccgcaca ccgtggcctt
     1261 tttgtgtaac ccggtaaagc gggcgtttcg gaaattctga caatggcgcg atgtgcatgt
     1321 aaagctggta cagcgtcagc caggatgatt catcctgctc gtctggcttg tacactgatt
     1381 ttaccagcac aaaggtaccg gactggcgca gggggcgttc ctgataacat tcgatagccg
     1441 cgtaatcgcg gttaacccgc catgccacca cttcgccatc catcatgaac tggagcggca
     1501 ccgatttttc aggtgtttcc tgattcagca ccgaggccgg ggacgatgac ctcccgacat
     1561 gaatacctcc gtgccagtaa cgatttacgc caagtagcca ccaaccgtgg gcgtttttgc
     1621 ggatgtcggt tagcagctcg tcatagctct tatagtctcg tccatcggct ttccgtaccg
     1681 gataacgtat gttcatcatc ttttcttaag tgaaatcaca tatttcgatg gattctcgcg
     1741 cgtattttgg gtttggggtg tagtggaacc aaaaagacgg ttaagcggtt aaattgaatg
     1801 gtcaataaca ggtaaatcaa tctgttgaga tcaacgacgt cttttgctcc gcaaacggct
     1861 aagatgagta cagataattt ggttatcgta aaaaaggtgg ctaaaaattt caacctgcaa
     1921 tgtaaattgg ccggttgatt tttggctatt taaattgacc tcttaacggc gtttattgtt
     1981 ccattgctcc ccaaccccct atggattata agccgcacat acattacttc gttctgcata
     2041 tcaccactaa tctgcttaag atgtactaac aacatcatcg cctgttggct aaacggaact
     2101 ataactcctc tttcattttc atgccgcgat acaagtaatg cacaccttta atttcttcac
     2161 gttttgccgg aatgcgccaa atagcgttat caaaatcaaa ttctttttaa cgagcaaaac
     2221 gccagtccga agactggcgc agtgataaaa cattctgcat catgccacat tctgctcctg
     2281 aagatgacgc agacgatggt cccagtttcc caggtaatgc ccgggagtca gcagtacgcg
     2341 gcgtcgctca ttacagagcg agttaccaat caccacttct ccggccacac cacacagcga
     2401 cagttgtata tacgccatgc cggctgccag cgggtcaata tccgttgccg acacccacat
     2461 cctgtggtat gccggatacc ccgaacggtt cagggtgtca accattgcca gaatcatact
     2521 gccggcacca caggcaggtt cactgagcgt gataaagggt ttactctcaa acaacgcctt
     2581 cacatttccc agttgcatct gtgccatcat gctggcaaca ctccagggcg tgaaaaactg
     2641 actccggtat ttatccccca gttccagttg catgaacaca ctacccagaa aatcacagaa
     2701 tccctgctcc agtccctcaa tgacatgagc aagcaaatgc gcaatgtgga taacatcctg
     2761 cttttcataa ccactgacga ttcgcagata cttctgttcc cgggcctcac tgaactgaag
     2821 ccggttttcc agggcaatca cactacagct gacaaagtct tcaaacacct tatgacgttt
     2881 gtgataacag gctgtccggt taaacaacga aatgaattcc ttctgatggt cagtcatggt
     2941 ggccatgttt tctccgtaaa aacagggaac acatggcccg gacaggggaa caggtgttcc
     3001 ccttcgggtt gaaatgttga taaaacgggc atcacggaca taatacccgg aggttaatta
     3061 ctgggctgag accagaggaa ccggtaaagg tgcggctttc gtctgttctt cag
//
LOCUS       DAZFLD010000333         3015 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.335, whole genome shotgun sequence.
ACCESSION   DAZFLD010000333 DAZFLD010000000
VERSION     DAZFLD010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3015)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3015)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3015
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.335"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..840
                     /locus_tag="ACHKBL_004714"
     CDS             <1..840
                     /locus_tag="ACHKBL_004714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2345 domain-containing protein"
                     /protein_id="HFO5821453.1"
                     /translation="LENAAERAQALKADIDSQIQMFEQRLKPLNEAVLFSAPEGIALT
                     SGEDVQLAAGQNLAVNAGGDISTGTMGNLTALAGERLGLFARSGQLSVKSGEGPVEVQ
                     AQNGSMRLFAEKKLTLSSASDISFAGKKRITLIGGGSYLRLEAGKIEYGTTVSYLRKV
                     KRTIAAGAASQQPEMPWLADPVKPSASALMQYLYHDDVPVVGAPFKATLADGTVREGN
                     LDGAGYLHLEDVPPGRVLVELGPDTRDYLRKNKTDNRLFVGTSLSEADIDSLIAKYSE
                     GVE"
     gene            837..2381
                     /locus_tag="ACHKBL_004715"
     CDS             837..2381
                     /locus_tag="ACHKBL_004715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002445136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5821454.1"
                     /translation="MSGVNVAWFKETSLETGQWIWGTLQGAFNEKQTIGQIIVDAVIG
                     MIPLVGDVTAVRDLIAVSIGLAKEPKKRESVTEWVFLVILLFALIPVIGGVIKGVGRL
                     ALRVTTKVAENVETLGAVIKFLNRIGEGDAVKWLNKLNVVEYQAQLIEKMGDFCNTMI
                     TAINKILKARVGRMLPEKWQADLLLVRDGFAALQRLASRMIPQALKELDAKLRVLQAL
                     VYKGEIHTITTGMGRAEVRREAEAYLIERPLCEAPKQGTRYPSVHAEVIHEDRLRLKY
                     SREGYPDLLGYKGKSPAFGDEQVFTNIASFSGEITAKSASEMGGSTIFRAFGNPTELA
                     PNGSWAAGGFWGYQKVPGSAEEWRQLSAVLDEWNGNGFLVVLHLPEDLATRMPGSNAW
                     HGKIAEQFGQKRPDQYLQGGGEQVIINLDETLKTELNRIGEQVKASGQAVSIEINGVN
                     AEFYPTGWKEVEGVHGYSKPEGIEPPYVTYTRELHDDELRSKVTERTILNAARIEYQT
                     TTGSEQ"
     gene            2378..>3015
                     /locus_tag="ACHKBL_004716"
     CDS             2378..>3015
                     /locus_tag="ACHKBL_004716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002445110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Imm72 family immunity protein"
                     /protein_id="HFO5821455.1"
                     /translation="MITIEKTKYPMSDEQVRRKLFWVLQRLSSYSLWQRKRDAWAYFT
                     QQYEQALKNWPEEITKGFDPEAIIWAYDALRLYDEGLSELAVGNRQVWQRLTGEFHQL
                     ARPVDLIESYFYLPCHERGVQQEPYPVEVEKLNKLKIAAKIHGDYLMYPPHNNVCNFF
                     DAAYLLELDNYKYNFNKLAYPVFPENLPPVPERSDIIIKTDDPVPCDGIWEPV"
BASE COUNT          800 a          608 c          922 g          685 t
ORIGIN      
        1 ctggaaaatg ccgcagaacg ggcacaggcg ctgaaggctg atattgacag tcagatacag
       61 atgtttgagc agcggctgaa accgctcaat gaagccgtgc ttttctcggc gccggaaggc
      121 attgccctga ccagcggtga agatgtgcag ctggctgctg gtcagaacct ggctgtcaac
      181 gccggaggtg atatcagtac cggaacaatg ggcaacctga ccgcgctggc cggagagagg
      241 ctggggcttt ttgccaggag cggacaactg agcgtcaaat ccggggaggg gccagtagag
      301 gtacaggcgc aaaacggcag catgcggttg tttgctgaga agaagctgac gctgagttcg
      361 gcaagtgata tctcctttgc cgggaaaaaa cgtatcacgc tgattggcgg ggggagttac
      421 ctgcggctgg aggcggggaa aatagagtac ggtacgacgg tgtcgtatct gcgcaaagtt
      481 aaacggacta tagcggcggg ggctgcgtca cagcagccgg aaatgccttg gcttgccgac
      541 ccggttaaac cctctgccag tgcgctgatg cagtatctgt atcacgacga tgtcccggta
      601 gtgggcgcgc ctttcaaagc gactcttgct gatggcactg tacgggaagg taaccttgac
      661 ggtgcgggat atctgcatct ggaagacgtc ccgccaggga gagtgctggt ggagctcggg
      721 ccggatacca gagattattt acgtaaaaat aagaccgata atcggttgtt tgttgggaca
      781 tctctcagcg aggctgacat tgactcactg atagcgaagt acagcgaagg tgtggaatga
      841 gtggagtaaa cgttgcctgg tttaaggaaa ccagcctgga aaccggacag tggatctggg
      901 ggacgcttca gggggcattt aatgaaaaac agaccattgg gcagatcatc gttgatgcgg
      961 tgatcggcat gatcccgctg gtcggcgatg taacggcagt gcgagatctg atagcggttt
     1021 cgataggact ggcaaaagaa ccgaagaaac gggaaagtgt gactgaatgg gtgtttctgg
     1081 tgatcctgct gtttgcgcta atcccggtaa ttggtggcgt gataaaaggt gtcggacgtc
     1141 tggccctgcg tgtgacaaca aaagtggcag agaatgtgga aacccttggc gctgtgatta
     1201 agtttctgaa ccgtatcggc gaaggtgatg cggtgaagtg gctgaataag ctgaatgtcg
     1261 ttgagtatca ggcacagttg attgaaaaga tgggggattt ttgcaacacg atgattaccg
     1321 ccatcaataa aatcctgaaa gcccgggtcg ggcggatgtt gccggagaaa tggcaggcgg
     1381 atttactctt ggttcgggat ggttttgcgg cactgcaacg gctggcgtcg agaatgatcc
     1441 cgcaggcgtt gaaagagctg gatgcgaaac tgcgggtgtt gcaggcgctg gtttataaag
     1501 gtgaaataca tacaatcacg acggggatgg gacgtgctga agtgcgccgt gaggctgaag
     1561 catatcttat tgagcgtccg ctgtgtgaag cgccgaagca ggggacgcgg tatccgagtg
     1621 ttcatgcgga ggtaattcac gaagacagat tgcggcttaa atattccagg gagggatatc
     1681 cagatttgtt gggatataaa ggaaaaagtc cggcatttgg tgatgagcaa gtatttacaa
     1741 atattgcctc ttttagcggc gagattaccg caaaaagtgc aagtgagatg ggcggtagta
     1801 cgatttttcg cgcttttggt aatccaacag aattagcacc caacggttct tgggcggctg
     1861 gcggattctg ggggtatcaa aaagtgccgg gcagtgctga agaatggcgt cagctttcag
     1921 ctgtactgga tgagtggaat ggtaacggtt ttctggtggt gctgcatttg ccagaagacc
     1981 tggcaactcg tatgccgggt agcaatgctt ggcacgggaa aattgctgaa cagttcggac
     2041 agaagagacc tgaccagtat ctgcaaggtg gtggcgaaca ggtaattatt aatctggatg
     2101 aaacgttgaa aacagagttg aaccgcatcg gggagcaggt aaaagccagc ggtcaggctg
     2161 tcagtattga aattaatggc gtaaatgccg agttttatcc gacgggctgg aaagaggtgg
     2221 aaggcgtgca tggttacagt aagccggaag ggatagaacc gccgtatgtg acgtataccc
     2281 gtgaactgca tgacgatgaa ctgcgcagca aagtgacaga gcgaacgata cttaatgcgg
     2341 cccgcattga gtaccagacc acgacaggaa gcgaacaatg attaccattg aaaaaacaaa
     2401 atacccgatg agcgatgagc aggtacgtcg caaattattc tgggtgttac agcggctgag
     2461 cagctatagc ctttggcagc gcaaacggga tgcctgggcc tattttaccc agcagtatga
     2521 acaggcgctg aaaaactggc cggaagagat aacgaagggg tttgatcccg aggcgataat
     2581 ctgggcatat gatgctttgc gtctttacga tgaagggcta tcagaattgg cagtgggaaa
     2641 tcggcaggta tggcagcgtt taacgggaga atttcatcaa cttgctcgcc cagtggattt
     2701 aattgaatct tatttttatc tcccctgcca tgaacgaggt gtacagcaag aaccataccc
     2761 ggtagaagta gaaaagctta ataagctgaa gattgcagca aaaatacatg gtgactatct
     2821 aatgtatcca ccacataaca atgtatgcaa ctttttcgat gctgcatatt tattagaatt
     2881 agataattat aaatataatt ttaataagtt agcgtatccg gttttcccag aaaatctccc
     2941 ccctgttcca gaacgcagcg atattattat taaaaccgat gacccggttc cctgtgacgg
     3001 catctgggaa ccggt
//
LOCUS       DAZFLD010000334         3010 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.336, whole genome shotgun sequence.
ACCESSION   DAZFLD010000334 DAZFLD010000000
VERSION     DAZFLD010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3010
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.336"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            164..1015
                     /locus_tag="ACHKBL_004717"
     CDS             164..1015
                     /locus_tag="ACHKBL_004717"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001195265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFO5821456.1"
                     /translation="MQKVKLNNGIEMPLLGFGVFQMTDAAECERAVIDAINSGYRLID
                     TAASYQNEIQVGNALKQSGIARNELFVTTKLWLQDTSYEGAKAQFERSLNRLQLDYVD
                     LYLIHQPYGDVHGAWRAMEELQQAGKIRAIGVSNFHPDRLADLIAFNNVVPAVNQVEV
                     NPFNQQLQAVPWMQSRGIQPEAWAPFAEGKNGLFQHPVLTAIGEKYGKSVGQVVLRWI
                     YQRGIVSLAKSVRKERMEENINILDFELSPEDMLQITALDTATSAFFSHRDPAMVEWL
                     TGRKLDV"
     gene            1042..2031
                     /locus_tag="ACHKBL_004718"
     CDS             1042..2031
                     /locus_tag="ACHKBL_004718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001172283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFO5821457.1"
                     /translation="MQKRYLGKSGLEVSALGLGCMGLSHGYGPATDTRQAIELIRAAV
                     ERGVTFFDTAEVYGPYLNEEVVGEALKPFRDRVVIATKFGFTFGDDNKQQILNSRPEH
                     IREAVEGSLRRLKTDVIDLLYQHRVDPDVPIEDVAGTVKDLIAEGKVKHFGLSEAGAQ
                     TIRRAHAVQPVTALQSEYSMWWREPEQEILPLLEELGIGFVPFSPLGKGFLTGAIKPG
                     TTFGKDDYRSTVPRFAAQSIEANEKLVTLLGELAAEKGVTSAQIALAWLLAQKPWIVP
                     IPGTTKLHRLEENLAAADIVLSQKDTQQISEALETIKIVGERYSPEHQARVGR"
     gene            complement(2062..2955)
                     /locus_tag="ACHKBL_004719"
     CDS             complement(2062..2955)
                     /locus_tag="ACHKBL_004719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO5821458.1"
                     /translation="MLKENFNELQIFLVVARERSFTKAAGKLGVSQSALSHAIKALEE
                     RLNIRLLTRTTRSVAPTEAGERIIACLEPRIDELEQELESLIQLNGTPSGNIRLSAGE
                     HAARSLVWPKLKPFLREYPEINVELVVDNGFVDIVEGRFDAGIRLGENVDKDMVAVRI
                     GPDMRMAVVGAPAYFAANPAPETPHELQNHRCINMRLPTAGGLYHWEFEREGKPLRVK
                     VDGQLTCSPLPERIDAALSGFGIACVPEDMVQEYIESGKLIQVLQEWCPTFPGYYLYY
                     PSRKQHPPAFALLIDALRYTE"
BASE COUNT          782 a          781 c          733 g          714 t
ORIGIN      
        1 ttcatcggac tgtaaattcc ggggatatcg aacttgcacg actaatctca accctcaaac
       61 agatttatga attttgttca taacccctga caaatcctgc cgtctaatcg agctcttccc
      121 tttacgttat gcttctcatg aacgataacg gaaggataac accatgcaaa aagtaaaact
      181 gaacaacggt attgaaatgc ccctgttggg ctttggtgta tttcagatga ccgatgccgc
      241 cgaatgcgaa agagcggtca ttgacgctat taacagcggt tatcgactga ttgacaccgc
      301 cgcctcttat caaaatgaaa tccaggtcgg gaacgcactg aaacaaagcg gtatcgcccg
      361 taacgaactc tttgtcacca cgaaactctg gttgcaggat accagttacg aaggggccaa
      421 agcccagttc gaacgctctc tgaatcgact gcaacttgat tacgttgacc tgtacttgat
      481 tcaccagcca tatggcgatg tccacggagc ctggcgtgcc atggaagagc tgcaacaggc
      541 cggtaaaatt cgcgctattg gcgtcagtaa cttccatcct gaccgactgg cagaccttat
      601 cgcttttaat aacgtcgttc cggcggtaaa ccaggttgaa gttaaccctt tcaatcagca
      661 gctacaggcc gttccctgga tgcaaagtcg tggcattcag ccagaagcct gggcaccgtt
      721 tgcggaaggg aaaaatggtc tgttccagca tcctgttctc acggcaattg gcgagaagta
      781 tggcaaaagc gtgggccagg ttgttctgcg ctggatctac caacgaggca ttgtttcact
      841 ggcaaaatca gtgcgaaaag aacgcatgga agagaacatc aacattctcg attttgaact
      901 cagccctgaa gatatgctgc aaattaccgc cctggatacc gcaaccagcg cgttcttctc
      961 tcaccgcgat cccgcaatgg tggaatggct gactggccgc aaacttgatg tttaagccgc
     1021 gataaaagag gtattcattc aatgcaaaaa cgttatctgg gtaaatccgg actcgaagtc
     1081 tccgcccttg ggctcggttg catgggctta agccacggct acggcccggc gaccgatacc
     1141 cgtcaggcta tcgaacttat tcgtgctgcg gttgaacgtg gcgtcacctt cttcgatacc
     1201 gccgaagtgt atggtccgta tctgaatgaa gaagtggtcg gcgaagcctt aaaaccgttt
     1261 cgtgaccgcg tggtaattgc cactaaattc ggttttacct tcggcgatga taataagcag
     1321 cagattttaa acagccgtcc ggaacatatc cgtgaagccg ttgaaggatc attacgtcgt
     1381 ctgaaaacag atgttattga cctgctgtat caacaccgtg ttgatccaga tgttcctatt
     1441 gaagatgttg caggaacggt gaaagacctc atcgcagaag gtaaggtcaa acatttcggc
     1501 ctgtctgaag caggagcaca aaccattcgt cgtgcacatg cggtacaacc tgtcaccgcc
     1561 ctgcaaagcg aatattccat gtggtggcgt gagcctgaac aggaaattct gccgttgctg
     1621 gaggaactgg gaattggttt tgtccccttc agcccactag gtaaaggttt cctgacaggg
     1681 gcgattaagc cgggaaccac tttcggcaaa gatgattacc gcagcaccgt accgcgtttc
     1741 gcagcacaat ccattgaagc caatgaaaaa ctggtcacct tgttaggtga actggctgcg
     1801 gagaaagggg taacgtctgc acaaatcgca ctggcatggc tattagcaca aaagccgtgg
     1861 attgttccca tccctggcac cactaaactg caccggctgg aagaaaacct ggccgctgct
     1921 gacattgttc tttcgcagaa agatacgcag cagataagcg aggcgctgga aacaattaaa
     1981 atcgtcggtg aacgttactc gcctgaacat caggcacgcg taggccgcta atacccaaac
     2041 gggtccagga taaaggaccc gttattcggt gtagcgaagc gcatcgatca acagcgcaaa
     2101 agctggcgga tgctgcttgc ggctggggta gtaaagataa tacccaggaa aagttggaca
     2161 ccactcctgt aacacctgaa taagcttgcc tgactctata tactcctgaa ccatgtcttc
     2221 agggacacaa gcgatcccaa accctgataa cgcggcatca attctttctg gcaatggact
     2281 acacgtcagt tgcccatcaa ccttaacgcg aagcggtttc ccttctcgct cgaactccca
     2341 gtggtaaagc ccaccggcag ttggcaaacg catgttgatg cagcgatgat tttgtagctc
     2401 gtgaggcgtt tcaggcgcag ggtttgcagc aaaatacgcc ggagctccca ccacagccat
     2461 ccgcatatct ggtccaatcc tgactgcaac catatcttta tcaacatttt cacccagtcg
     2521 tatcccggca tcaaaacggc cttcaacaat atcgacaaaa ccgttatcca ccaccagttc
     2581 gacattgatt tcgggatact ctctgagaaa aggttttagc ttcggccaga ccagacttcg
     2641 cgcggcatgt tccccggcag ataaccgaat attcccggag ggcgtgccgt tcaattgaat
     2701 aagtgattcc agttcctgtt caagctcgtc aatacgcggt tcaaggcagg caatgattct
     2761 ctcccccgct tctgtgggag ccacactgcg ggtagtccgt gtcaggaggc ggatattgag
     2821 cctttcctcc agagccttta ttgcatggct aagtgcagac tgagagacgc cgagtttgcc
     2881 cgctgctttg gtaaaacttc gctctcttgc caccacaaga aagatttgca gttcgttgaa
     2941 gttttctttg agcattggcg atttccttgt agaagcatcc tcttcacaca cactcatgaa
     3001 gggggcatag
//
LOCUS       DAZFLD010000335         3002 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.337, whole genome shotgun sequence.
ACCESSION   DAZFLD010000335 DAZFLD010000000
VERSION     DAZFLD010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3002
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.337"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(228..304)
                     /locus_tag="ACHKBL_004720"
     tRNA            complement(228..304)
                     /locus_tag="ACHKBL_004720"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(268..270),aa:Asp,seq:gtc)
     gene            complement(437..1168)
                     /gene="dnaQ"
                     /locus_tag="ACHKBL_004721"
     CDS             complement(437..1168)
                     /gene="dnaQ"
                     /locus_tag="ACHKBL_004721"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HFO5821459.1"
                     /translation="MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLT
                     GNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNAAF
                     DIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRT
                     LHGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQDETTIQRIVRQASKLRVVFAT
                     DEELAAHEARLDLVQKKGGSCLWRA"
     gene            1233..1700
                     /gene="rnhA"
                     /locus_tag="ACHKBL_004722"
     CDS             1233..1700
                     /gene="rnhA"
                     /locus_tag="ACHKBL_004722"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HFO5821460.1"
                     /translation="MLKQVEIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTN
                     NRMELMAAIVALEALKEHCEVILSTDSQYVRQGITQWIHNWKKRGWKTADKKPVKNVD
                     LWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV"
     gene            complement(1697..2419)
                     /locus_tag="ACHKBL_004723"
     CDS             complement(1697..2419)
                     /locus_tag="ACHKBL_004723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFO5821461.1"
                     /translation="MKPARVPQTVVAPDCWGDLPWGELYRKALERQLNPWFTKMYGFH
                     LLKIGNLSAEINCEACAVSHQVNVSAQGLPVQVQADPLHLPFADKSVDVCLLAHTLPW
                     CTDPHRLLREADRVLIDDGWLVISGFNPISLMGLRKLVPVLRKTSPYNSRMFTLMRQL
                     DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQ
                     SKNKPRIRQAVGATRQCRKPQA"
     gene            2453..>3002
                     /gene="gloB"
                     /locus_tag="ACHKBL_004724"
     CDS             2453..>3002
                     /gene="gloB"
                     /locus_tag="ACHKBL_004724"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004416 - hydroxyacylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019243 - methylglyoxal catabolic process
                     to D-lactate via S-lactoyl-glutathione [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HFO5821462.1"
                     /translation="MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAIAANNW
                     QPEAIFLTHHHHDHVGGVKELVKKFPQIVVYGPQETQDKGTTQVVKDGETAFVLGHEF
                     SVIATPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTASQMYQSLKKLSALPDDTL
                     VCCAHEYTLSNMKFALSILPHDL"
BASE COUNT          810 a          723 c          703 g          766 t
ORIGIN      
        1 atgaaatata cattcattta atcaatataa aattatcctc taaaaatata tttaagttga
       61 atggttcgat atcattattg aattttaata aatgagacaa caagcgagag ttacgcactt
      121 acgccagagt aaaatcattg aacagagaaa tggcgggatt caaatacaaa aaaaccgcaa
      181 aaataaaaat ttttgcggtt ctctgaactc attttcatga gtgaatttgg cggaacggac
      241 gggactcgaa cccgcgaccc cctgcgtgac aggcaggtat tctaaccgac tgaactaccg
      301 ctccgcgttt tgttccgttg ggaacgaggc gaatattacg gattgcctca cccttcgtca
      361 acggtttttc tcatcttttg aatcgtttgc tgcaaaaatc gcccaagtcg ctatttttag
      421 cgccttttac aggtatttat gcccgccaga ggcagcttcc ccccttcttc tgcaccagat
      481 cgagacgggc ttcatgtgct gcaagctctt catctgtcgc aaaaacaacg cgtaacttac
      541 ttgcctgacg cacaatgcgc tgaattgttg tttcatcttg ttgctgttgt gtctctcctt
      601 ccatcgcaaa agccatcgac gtttgcccac cagtcatcgc cagataaact tccgcaagga
      661 tctgggcatc gagtaatgcc ccgtgcagcg ttcgtttact gttatctatt tcgtagcggg
      721 cacataacgc atcgaggctg ttgcgcttac cgggaaacat tttcctcgcc accgcaaggc
      781 tatcggtgac cttacagaag gtattggtct tcggaatatc gcgcttaagc aacgaaaact
      841 cgtagtccat aaagccgata tcgaacgctg cgttatggat caccagctcc gcgccgcgga
      901 tatagtccat gaactcatcg gctacctctg caaacgtggg cttatcgagc aaaaattcat
      961 cggcaatacc atgtacgcca aaggcttccg gatccaccag ccgatcgggt ttgagataaa
     1021 catggaagtt attgcccgtc aggcgacggt tcaccacttc aacggcacca atctcaatga
     1081 tcttgtggcc ttcatagtgc gcaccaatct ggttcatacc ggtagtttcg gtatcgagaa
     1141 cgatctggcg tgtaattgca gtgctcatag cggtcattta tgtcagactt gtcgttttac
     1201 agttcgattc aattacagga agtctaccag agatgcttaa acaggtagaa attttcaccg
     1261 atggttcgtg tctgggtaat cctggacctg ggggttacgg cgctatttta cgctatcgcg
     1321 gacgcgagaa aacctttagc gcaggctaca cccgcaccac caacaaccgt atggagttga
     1381 tggccgctat tgtcgcgctg gaggcgttaa aagaacattg cgaagtcatt ttgagtaccg
     1441 acagccagta tgtccgccag ggtatcaccc agtggatcca taactggaaa aaacgtggct
     1501 ggaaaacagc agacaaaaaa ccagtaaaaa atgtcgatct ctggcaacgt cttgatgctg
     1561 cgctggggca gcatcaaatc aaatgggaat gggttaaagg ccatgccgga cacccggaaa
     1621 acgaacgttg cgatgaactg gctcgtgccg cagcgatgaa tcccacactg gaagatacgg
     1681 gctatcaagt cgaagtttaa gcctgtggtt tacgacattg ccgggtggct ccaaccgcct
     1741 ggcgaattcg tggcttgttt ttactctgtt tcatcggatt tagcgttaaa ggaatggtcc
     1801 gtttccgggc aacaataagt tgtaagcaac caagcgcagg aatatgcgca ttcaatagtt
     1861 ttcctccgtg tttgttccac gggagaacgt ggaaacggct ggcgtgtagc acttcaaaat
     1921 tcaacaaaga gagccagtcc agctgccgca tcagagtaaa catccggctg ttatagggcg
     1981 aggttttgcg caataccggc acaagtttgc gtaatcccat taaactgatg ggattgaagc
     2041 cactaatcac cagccagcca tcatcaatca atacccgatc ggcttcacgc aataaacgat
     2101 gcggatcggt gcaccacggc aatgtatgtg ccagtagaca aacatcaacg gatttatcgg
     2161 caaaaggaag atgaagtggg tccgcctgca cctggacggg cagtccttgc gcagaaacat
     2221 tcacttgatg agaaaccgcg cacgcttcgc aattgatttc tgcgcttaaa ttgccaatct
     2281 taagcagatg aaaaccatac attttagtga accacgggtt gagctggcgc tccagcgcct
     2341 tgcgataaag ctctccccag ggcaaatcgc cccagcaatc aggagccacg acagtttgag
     2401 ggactcttgc cggtttcatc acaaccttcc gtttcacact gagaggtaat ctatgaatct
     2461 taacagtatt cccgcctttg atgacaatta catctgggtt ttgaatgatg aagcaggtcg
     2521 ctgcctgatt gtcgatcccg gagacgcaga gccagtatta aacgccattg ccgccaataa
     2581 ctggcaaccg gaggccatat ttctcaccca ccatcaccac gatcacgttg gcggcgtaaa
     2641 agaactggtg aaaaagtttc cgcaaattgt ggtgtatggt ccacaagaga cacaagataa
     2701 gggaacaaca caggtagtca aagatggcga aactgccttc gttttggggc atgaatttag
     2761 tgtaattgcc acgccgggtc acactttagg acatatctgt tacttcagta aaccttatct
     2821 attttgcggc gacacgctgt tttctggtgg gtgtggtcgg ttgtttgaag ggacagcatc
     2881 acaaatgtat caatcactta aaaagttaag tgcgttacct gacgatacat tggtatgttg
     2941 tgctcatgaa tataccttat caaatatgaa gtttgctttg agtattcttc cgcacgattt
     3001 gt
//
LOCUS       DAZFLD010000336         2985 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.338, whole genome shotgun sequence.
ACCESSION   DAZFLD010000336 DAZFLD010000000
VERSION     DAZFLD010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2985
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.338"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            360..878
                     /locus_tag="ACHKBL_004725"
     CDS             360..878
                     /locus_tag="ACHKBL_004725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
                     /protein_id="HFO5821463.1"
                     /translation="MPTPCYISITGQTQGNITAGAFTADSVGNIYVQGHEDEMLVQEF
                     LHNVTVPTDPQSGQPAGQRAHKPFIFTVALNKAVPLMYNALASGEMLPTTELHWWRTS
                     VEGKQEHYFTTRLTDSTIVDMKLHMPHCQDPAKREFTQLLEVSLAYRKIEWEHVKSGT
                     SGADDWRAPLEA"
     gene            1088..>2985
                     /locus_tag="ACHKBL_004726"
     CDS             1088..>2985
                     /locus_tag="ACHKBL_004726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HFO5821464.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLNQSLSSLFSLDLSLVSQQ
                     FLSLEFAQVLDKMAYLTVWQGDDVQRRVKGVVTWFELGENDKNQMLYSMKVCPPLWRT
                     GLRQNFRIFQNEDIESILATILKENGVTEWSPLFSEPHPSREFCVQYGETDYDFLCRM
                     AAEEGIFFYEEHAQKSTDQSLVLCDTVRYLPESFEIPWNPNTRTEVSTLCISQFRYSA
                     QIRPSSVVTKDYTFKRPGWAGRFDQEGQHQDYQRTQYEVYDYPGRFKGAHGQNFARWQ
                     MDGWRNNAEVARGTSRSPEIWPGRRIALTGHPQANLNREWQVVASELHGEQPQAVPGR
                     RGSGTTLNNHFAVIPADRTWRPQPLLKPLVDGPQSAVVTGPAGEEIFCDEHGRVRVKF
                     NWDRYNPSNQDSSCWIRVAQAWAGTGFGNLAIPRVGQEVIVDFLNGDPDQPIIMGRTY
                     HQENRTPGSLPGTKTQMTIRSKTYKGSGFNELKFDDATGKEQVYIHAQKNMNTEVLNN
                     RTTDVINNHAETIGNNQVIAVTNNQIQTVGVNQIETVGSNQIIKVGSVQVETIGLVRA
                     LTVGVAYQTTVGGIMNTSVALMQSSQIGLHKSLMVGLGYDVKVGNNVTFTVGKTKKDD
                     TGQTAIYSA"
BASE COUNT          760 a          737 c          831 g          657 t
ORIGIN      
        1 ttttgtgatt gattaagtgg gtatttttaa ggaatatctc aaaaattatc aaaatgataa
       61 ttcaaaaagg ataaatacaa tatttattca tgattcagct ttggccattt tatggaggca
      121 atttggactt tgaacagttg ggtatggtta tccagatata tattattaac ttattgctga
      181 ataagagatt taatgatttt aaaaatagtt tttacgacaa ccgtaaattc tagctcggtt
      241 tgtatgtaaa aaatactgaa aaatgatgcg gttccgtgaa gagagctgca tctgcctgtc
      301 tgggattaac ggcaggtcat tgtaagtagt aattaaccac gttaacaagg agtttacaaa
      361 tgccaacacc gtgttatatc tccatcactg gtcagaccca gggaaatatt actgcaggtg
      421 catttaccgc tgattctgtc ggcaacatct atgtgcaggg gcacgaagac gagatgctgg
      481 tgcaggaatt cctgcataac gttactgtcc cgaccgatcc tcagtccggc cagcctgcgg
      541 gtcagcgtgc tcacaagccg ttcattttta ccgtggcgct gaacaaagct gttccgctga
      601 tgtacaacgc actggcctcc ggcgaaatgc tgccaaccac tgaacttcac tggtggcgta
      661 cctctgtgga aggcaaacag gagcattatt tcaccacccg cctgaccgat tcgacgattg
      721 tcgatatgaa actgcatatg ccgcattgcc aggatccagc gaaacgcgag ttcacccagt
      781 tgctcgaagt ttcactggcc taccgcaaaa tagagtggga acacgtcaag tctggcacct
      841 ccggtgccga cgactggcgc gcaccgctgg aagcataagt taagccaaca gcatccggct
      901 atcaggccgg atgctgtttt tgtattacgc cgtaaaaaga aaggttgtca gtaagggatt
      961 acgttcacgg gagatggcgg atgccggaaa cttcggcggt agcgtgcctg aacgcaagcg
     1021 taacactgta ctgttacagc cgctttaaca gcgaactgtc tgtttttcct taaacccgga
     1081 ggttgctatg tcaaccggat tacgtttcac gctggaagtg gacggtctgc caccggacgc
     1141 gtttgcggtg gtctcctttc atctgaacca gtcactctct tcgctctttt ccctcgatct
     1201 ctctctggtc agccagcagt ttctctccct tgaatttgcg caggtgctgg acaaaatggc
     1261 ctacctgacg gtatggcagg gcgatgacgt acagcgccgg gtgaaaggtg tggtgacctg
     1321 gtttgaactg ggggagaacg acaaaaacca gatgctgtac agcatgaagg tgtgcccacc
     1381 gctgtggcgc acagggctgc gccagaactt ccgtatcttc cagaatgagg acatcgaaag
     1441 catactcgcg acgatcctga aagaaaacgg ggtgaccgag tggagtccgc tgttcagcga
     1501 gccgcatcct tcccgtgagt tttgtgtcca gtacggcgaa accgattacg atttcctgtg
     1561 ccggatggcg gcggaggaag gcatcttctt ttatgaggag cacgcgcaaa aaagtaccga
     1621 ccagagcctg gtcctgtgcg acaccgtgcg ttatctgccg gagtcctttg agatcccctg
     1681 gaatccgaac acccgtaccg aggtgagcac cctatgcatc agccagttcc gctacagcgc
     1741 acaaatccgc ccatcttccg tggtgaccaa agactacacc tttaaacgac ccggctgggc
     1801 agggcgtttt gatcaggaag gccagcacca ggattaccag cgcacacagt atgaagtgta
     1861 tgactacccc ggacgtttca agggtgccca cgggcagaac tttgcccgct ggcagatgga
     1921 tggctggcga aacaacgcag aagtggcgcg cggaacaagc cgttcgccgg agatatggcc
     1981 gggacggcga attgcgctga cggggcatcc gcaggcgaac ctgaaccggg aatggcaggt
     2041 ggtggcaagt gaactgcacg gcgagcagcc gcaggcggtg ccgggacgca ggggttcagg
     2101 taccacgctg aataaccact ttgcggtaat accggcagac agaacatggc ggccacagcc
     2161 gttgctgaaa ccgctggtgg acggcccgca gagcgccgtc gtgacgggac cggcaggaga
     2221 agaaatcttc tgtgatgaac atggccgcgt gcgggtgaaa tttaactggg accgctataa
     2281 cccgtcaaac caggacagtt catgctggat ccgtgtggca caggcgtggg caggcaccgg
     2341 ctttggcaac ctggcgatac cgcgtgtggg tcaggaagtc attgtggact tcctcaacgg
     2401 cgatccggac cagccgatta ttatggggcg tacctaccac caggaaaacc gcacccccgg
     2461 cagcctgccg ggaacaaaga cgcagatgac cattcgttcg aaaacctata agggcagcgg
     2521 gtttaatgaa ctgaagtttg acgatgcgac agggaaagaa caggtctaca tccacgcgca
     2581 aaagaacatg aacaccgagg tgctgaataa ccggaccacg gatgtgataa acaaccatgc
     2641 tgagaccatc ggcaacaatc aggtgattgc ggttaccaac aatcagatac agacggtggg
     2701 cgttaaccag atagagacgg tgggcagtaa ccagatcatt aaagtgggct ctgttcaggt
     2761 ggaaacgatt ggacttgttc gtgcgctgac cgtgggcgtg gcgtatcaga cgacggtagg
     2821 tggcattatg aacacctcgg tggccctgat gcagtcctcg cagatcggtt tgcataaatc
     2881 gttgatggtc gggctgggtt atgacgtcaa agtcggaaat aacgttacct tcaccgttgg
     2941 taaaacgaaa aaggatgata ccggacagac cgcgatttac tccgc
//
LOCUS       DAZFLD010000337         2958 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.339, whole genome shotgun sequence.
ACCESSION   DAZFLD010000337 DAZFLD010000000
VERSION     DAZFLD010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2958
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.339"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..183)
                     /locus_tag="ACHKBL_004727"
     CDS             complement(<1..183)
                     /locus_tag="ACHKBL_004727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001057978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5821465.1"
                     /translation="MNNCVEVLDNVHCEITESLSLLQVIIDSGDIGSPDALNMMNCLF
                     RSLSCTADKAEKHLCRT"
     gene            complement(224..586)
                     /locus_tag="ACHKBL_004728"
     CDS             complement(224..586)
                     /locus_tag="ACHKBL_004728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5821466.1"
                     /translation="MKDEYISAYRRRITVAALRRMKRKTGSNLLTVKRHDGELTTIEI
                     TEQFISQLLLRFEGVTRGEFGQKDGETAIRTAYQDAVGINQHGEYLTESGKLIIDELL
                     NECIDYIKEGHITRCVNH"
     gene            complement(761..961)
                     /locus_tag="ACHKBL_004729"
     CDS             complement(761..961)
                     /locus_tag="ACHKBL_004729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001539127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5821467.1"
                     /translation="MKTPLIMLEEVAAEIKENTSMLEFIFKNSGDNGETDDFLLCMIR
                     SMNKTCEKAYEYVDALRTNKGN"
     gene            complement(958..1149)
                     /locus_tag="ACHKBL_004730"
     CDS             complement(958..1149)
                     /locus_tag="ACHKBL_004730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000781553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5821468.1"
                     /translation="MKMFKGLTNEPETAFHHIAVLLEAGLIISASGDEECDELSDDIF
                     LLAQQYARSACDAFKEQRT"
     gene            complement(1151..1528)
                     /locus_tag="ACHKBL_004731"
     CDS             complement(1151..1528)
                     /locus_tag="ACHKBL_004731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000107960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO5821469.1"
                     /translation="MSTSQLMLIEQVESLLHESLESVSERNRHVLLEVGIEKIQQLKH
                     ILLPPRNKKDCRDGIRTQRESCGLSTAELATLLGVDEETIIQWENGKAEPLASQVIPL
                     ANILDCDPMWLLTSFHSDTTKES"
     gene            complement(1614..2273)
                     /locus_tag="ACHKBL_004732"
     CDS             complement(1614..2273)
                     /locus_tag="ACHKBL_004732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000349381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HFO5821470.1"
                     /translation="MDHDSFGGRLLMRRVQLGMSQDDLAKLTGVSRVTISKIELGDSQ
                     DTRSSNLFRIADALKCSPKWLLDGSAPSPTDSIQNVRNPKPVSPNRYRYPKLTWVSAG
                     PWNCEDYIAYPEEWIGSDIYAGDHGFWLEVKGDSMTSQNGEFSIFEGMNILVNPDKEV
                     FSGKYVVARLKNSGEATLKQYIEDAGQCFLKPLNPRYPLIPINGDCEIIGVVVEMRMQ
                     I"
     gene            2613..2906
                     /locus_tag="ACHKBL_004733"
     CDS             2613..2906
                     /locus_tag="ACHKBL_004733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001539121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5821471.1"
                     /translation="MSPDYVQMEMPARYSQADSEWIKRQLQSLPSSLRQKVALKYAEV
                     YEVTFDSEPVSFRKENRARHEANVRLRRFVETHGRALQGYTTQPPLAGTQQRT"
BASE COUNT          763 a          652 c          640 g          903 t
ORIGIN      
        1 agtccggcac agatgctttt ctgctttgtc tgctgtacag gataatgagc ggaaaagaca
       61 attcatcatg tttagcgcat ctggtgaacc aatatcaccg ctgtcaataa tcacctgtaa
      121 caaagacagg ctttctgtta tctcgcagtg gacattatcg agtacttcaa cgcaattatt
      181 catttttcat tatctccgtt attccggggg aggagctatg ctgttaatga ttaacgcatc
      241 tggtaatatg cccctcttta atgtaatcga tgcattcatt caaaagttca tcaatgatta
      301 actttcctga ttcagtcaga tactcaccat gttggttgat accgacagca tcctggtatg
      361 cagtgcgtat tgctgtttca ccatctttct gcccaaactc accacgggta acgccttcaa
      421 atcgtaatag caactggctt atgaactgtt ctgttatttc aatagttgtt aactctccat
      481 catgacgctt tacagtgagg agattacttc ctgtttttcg tttcatccgc cgcagtgctg
      541 caaccgttat gcggcgacga tatgcagaaa tgtattcatc tttcatttgt tatttcccgt
      601 atgccttttt gagaaacaat actgcaactg accagtatcc ggcatcagcc attaataaag
      661 cggttttata ggcatgtttg tctttcatgt gtcatcccca ttttaaaata gtgagaactc
      721 ccgaccgcag accgtcatgt ttttatgtat gattgtctgt ttaatttcct ttattcgttc
      781 tcagagcatc aacatactcg taagcctttt cacaggtttt attcatggaa cgaatcatac
      841 aaagtaagaa atcatctgtc tctccgttat caccagagtt tttaaatata aactcaagca
      901 ttgacgtgtt ttcttttatt tctgctgcca cttcctcaag cataatcagg ggagttttca
      961 tgttctttgt tccttaaatg catcgcatgc gcttctggcg tattgttgtg ccagtaaaaa
     1021 tatgtcatcc gaaagttcat cacattcttc atcaccggaa gccgaaatga ttaaccccgc
     1081 ttcaagcagt actgcaatgt gatgaaaagc tgtttccggt tcgttggtga ggcctttgaa
     1141 cattttcatc ttacgattcc tttgtcgtgt cagaatggaa acttgtaagt aaccacatcg
     1201 gatcgcagtc aagtatattt gccagaggaa tcacctggct tgccagaggt tcagccttgc
     1261 cgttctccca ctggatgatg gtttcttcat caactccaag cagcgtggcg agttcagcag
     1321 tggatagacc gcagctttcg cgttgggtgc ggatgccatc acggcaatct tttttgtttc
     1381 tcggaggtaa cagaatatgt tttagttgtt ggattttttc gattccaacc tcaaggagta
     1441 cgtgacggtt tctttccgat acagactcca gactttcatg aagcagcgat tctacttgtt
     1501 cgataagcat caattgtgaa gtagacataa aaaaacctcg taaccaatat ttcgaaatct
     1561 aggttacgag tgagtaatgt aggtgtcaag agcaattaat gttttttgtt gaattaaatt
     1621 tgcatacgca tctcaacgac aacaccaata atttcacaat ccccattgat ggggatcagt
     1681 ggatagcgtg ggtttagtgg ctttaggaaa cattgcccag cgtcttctat gtactgttta
     1741 agggttgcct ctccggagtt tttgagcctg gcgacgacat attttcctga aaaaacttct
     1801 ttgtcagggt taacaagaat gttcatgcct tcaaaaatag agaactctcc attctgtgat
     1861 gtcattgagt cccctttcac ttccagccag aagccgtgat cgcccgcata tatatcgcta
     1921 ccaatccatt cttctggata agctatgtaa tcttcgcagt tccaggggcc agcactcacc
     1981 catgttagtt tcggatatct ataacggttt ggagaaactg gcttaggatt tcggacgttt
     2041 tgaatgctat ctgtaggtga tggggcgctg ccgtcaagaa gccatttagg agagcatttc
     2101 aatgcatccg cgattctaaa taggtttgaa gatcttgtgt cttgtgagtc tcctaactct
     2161 attttgctga ttgttacacg cgagacaccg gtcaattttg ccaggtcatc ttgtgacatt
     2221 cctagctgta ctctgcgcat taacagtctg ccaccaaagc tatcgtgatc catcttctcc
     2281 cccttcttgt cgaaataatc gtttcgaaac aaatatagca aaaaatcttg aaatctaggt
     2341 ttcggaaaat taagattaat cgaaacaaac gtgtcgaggt ggatatggat ttgtatgaaa
     2401 ttctaaaaaa catgtttgga tcgaatgttg aaataggtcg ttattttccc cgaaggggaa
     2461 gggctagaac cggacaggct gtaggtaagt ggaaaacccg aggtgtgccg gaagacgtgg
     2521 cgatcctttg tcatctggat cccaaaattc cttatcagca tccgtcgctc atgaatgcca
     2581 gtcatgaatc gtgatgttct gccggaggct gtatgtcccc tgattacgtt cagatggaga
     2641 tgcctgccag gtacagccag gcagattcag agtggattaa gcggcagtta cagagcctgc
     2701 cttcctcact gagacagaag gtcgccctga aatacgcgga ggtttacgaa gttacttttg
     2761 attccgagcc tgtttcattc cgtaaggaga acagagcgag gcatgaagcc aatgtacggc
     2821 tgcgcagatt cgttgaaaca cacgggcgtg cattacaggg gtatacgact cagccgcccc
     2881 tggcaggaac gcaacagcgt acctgaatgg tgttggtctt aaaggtgacc gacgggctga
     2941 gaccccactt ctcaggat
//
LOCUS       DAZFLD010000338         2923 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.340, whole genome shotgun sequence.
ACCESSION   DAZFLD010000338 DAZFLD010000000
VERSION     DAZFLD010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2923
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.340"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>2923
                     /locus_tag="ACHKBL_004734"
     CDS             <1..>2923
                     /locus_tag="ACHKBL_004734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5821472.1"
                     /translation="AQSVRGTGEMARGLGDAMIQSPVKTGARILNEFSRMGLPGVATV
                     QDIFAGGSRGADEVIDTLPDGKNVVTDTVGKGLKATGKAVSDGAKASDEWLTEQMSPG
                     AVRALNTPITEGYDDSAVLLAKGVNLIGALVPDMVAGGVARKVGDVTLRKMLTAGLEK
                     KYIAAGMQPERATALAAEAVDKKMPDLFQAGLITHSTASAQGQSAMAAADAVLNADYS
                     ELAQSPKFQQTFLSIDADPQHAQLTDRQKMDLAKERVADEVRAQLATDPELLAVNAMA
                     AKLGDAQLFNLVTRGTAKTVKSGIVRNATEQGVINGAQNAYSRYQENMALRETAGMDV
                     SPWEGVTDAGIEGTVFGAAMGAPFGAVAGYRGRRQAAEEAAMRDAETVQQDDAAPQPE
                     PVDPVAQHRESMQGMNREQLLEQYADADMATEGDASAAHRREAASQLLNELDEQAKRQ
                     AVMNELKAKPRSELLEEYRRLSQKEERTETEEQQFQAIREVIRPQQEVTPEAQSQPEN
                     AEDGNGSIYPTVRFRDPNEVRIEINGNGASRPAERIEKVRPDNRYFTDEKSAMGSDVF
                     RNAAATGLKPSVVKKGENQYAVEMDNPAFSEDVATETINTLADGERIADADPMEQPAF
                     MRDPRFRGFTGDDTEVQARLARGNAPMAEELVRSQMAEGDAGPTAQELTERPRLPAPG
                     DIHPGQGYPLPGEVARTPDENQAGRGGRFTTTGEVKGQSFQKGRAPAPENAAGRQGET
                     LEGEIVRRGLPSPDAQNATAPVREGLPAPDIARNVRMPQPESLPRTVRDSLPELAQQA
                     EVRRQAGGNRDIPQPETIAPESETTVFTDRESTVRGGEVRGKKIEDFGEEIKGAAKHR
                     YAQLAETLGKTLEDRDYATQPLSKLFPKPDYAKLANEGADADTLAMIALYRSDIPAKT
                     KHNTAGWGESVKKVRHSVSEMLNGTVSAKRLAEWMEGRMPSRYADTWQLLRTLPPSQM
                     D"
BASE COUNT          747 a          719 c          919 g          538 t
ORIGIN      
        1 ttgcccagtc cgtccgggga acaggggaaa tggcccgtgg actcggtgat gcgatgattc
       61 agagcccggt aaaaacaggg gcgcgtattt taaatgagtt cagccgtatg gggctaccgg
      121 gtgtcgcaac tgttcaggat atttttgccg gtggcagcag gggggctgat gaggtcatcg
      181 ataccctgcc tgatggaaaa aacgtggtca ctgatactgt cggtaaaggt ctgaaagcaa
      241 ccggtaaggc tgtcagtgat ggtgccaaag cctcggatga atggctgaca gaacagatgt
      301 cgccaggtgc tgttcgtgcg ctgaatacgc cgataacaga aggctatgat gattctgcgg
      361 tcttgctggc gaagggtgta aacctgattg gtgcgcttgt acctgatatg gttgctggcg
      421 gtgtggctag aaaggtgggt gatgtcacac tgcgaaaaat gctgaccgcc gggctggaga
      481 aaaaatacat cgcggcaggg atgcagccgg aaagagccac ggcactggca gcagaagctg
      541 tcgataaaaa aatgccggat ttattccagg cgggcctgat cacccattcc acagccagtg
      601 cacaggggca gagtgcaatg gcggcagcag atgctgttct taatgcggat tactctgagc
      661 tggcgcagtc accgaaattt cagcagacgt ttttgtccat tgacgccgac ccgcagcatg
      721 cacagcttac tgatcgccag aaaatggatc tggcaaaaga gcgtgttgcc gatgaggtgc
      781 gcgcgcagct ggcaaccgat cctgaattgc tggctgtgaa tgccatggcg gcaaaactgg
      841 gtgacgcaca actgtttaat ctggtgacac gaggcacagc gaagaccgtt aaaagcggca
      901 ttgtcagaaa tgccacggaa cagggcgtaa ttaacggggc acagaacgcc tattcgcgct
      961 atcaggaaaa catggcattg cgtgagaccg ccggaatgga tgtatcgcca tgggaggggg
     1021 tgactgacgc agggattgaa ggcactgttt tcggtgctgc gatgggggct ccattcggtg
     1081 cggttgccgg atatcgtggc agacgtcagg ccgcagaaga agcagccatg cgtgatgctg
     1141 aaaccgtgca gcaggacgac gcagccccgc aacccgaacc ggttgatccg gtggcacagc
     1201 atcgtgaatc catgcagggc atgaatcgcg agcagcttct ggaacagtat gctgatgcgg
     1261 atatggcaac agagggtgac gcatccgcag ctcatcgccg ggaagctgcc agccagttgt
     1321 tgaatgaact ggacgaacag gcgaagcgac aggctgtgat gaatgagctg aaggcgaagc
     1381 cgcgttctga actgcttgag gaataccgca gactcagcca gaaagaggag cgcaccgaga
     1441 ctgaagaaca acagtttcag gcaatacgag aagtcattcg cccacaacag gaagtgacgc
     1501 cggaagcaca gtcacagcct gaaaatgcgg aggatggtaa cgggagcatt tacccgacgg
     1561 tgcggttccg ggacccgaat gaagtccgca ttgaaattaa cgggaatggt gcgtccagac
     1621 cagcggaacg cattgaaaag gtgcgcccgg acaaccgtta tttcacggat gagaaaagcg
     1681 ccatggggag tgatgttttc cgtaatgccg ccgctaccgg cctgaaaccg tccgtagtga
     1741 agaaaggcga gaatcagtat gccgttgaaa tggataatcc tgcgttctct gaagatgtgg
     1801 caacggaaac cattaacacc ctggctgacg gagagcgtat tgctgatgct gacccgatgg
     1861 agcagcccgc gttcatgcgt gacccgcgat tccgtggttt cacgggggat gatacggagg
     1921 tacaggcccg ccttgcccgt ggcaacgcgc cgatggcgga ggagcttgta cgttcacaga
     1981 tggctgaggg tgatgccggt ccgacagcac aggagttaac tgagcgtccg cgtctgcccg
     2041 cccccggcga tattcatccc ggacagggat atccgttacc gggagaggtg gcgcgtacgc
     2101 cggatgagaa tcaggccgga cgtggtggtc gttttaccac aaccggtgag gttaagggcc
     2161 agagtttcca gaaaggacgt gctccggcac cggaaaacgc cgctggtcgc cagggggaaa
     2221 cactcgaggg tgaaatagtt cgtcgtggtc tgccgtcacc ggatgcgcag aacgcgacag
     2281 caccggtacg tgaagggcta ccggctcctg acattgcgcg taatgttcgt atgcctcagc
     2341 ctgaatcact tccccgcact gtacgggact cactgcctga gctcgcacag caggcagaag
     2401 tacgccgaca ggccggagga aatcgtgaca tcccgcagcc tgagacaatc gcacctgaat
     2461 ctgaaacaac tgtctttact gacagggaat ctaccgtgcg cggaggtgaa gtcaggggca
     2521 aaaaaattga agactttggc gaggaaatta aaggggcagc caaacaccgt tatgcacagc
     2581 ttgctgaaac actgggtaaa acgctggaag acagggatta tgccacgcag ccgctgagca
     2641 aactgttccc gaaaccggac tacgcaaaac tggcgaacga aggtgccgat gctgacactc
     2701 tggcaatgat agcgctgtat cgtagcgata ttccggcgaa gacgaaacac aatacggcag
     2761 gctgggggga gagcgtaaaa aaagtacgac acagtgtatc ggaaatgctg aacggaacgg
     2821 tcagcgcgaa acgcctcgca gaatggatgg aaggcagaat gccctcccgt tacgcggata
     2881 cctggcaact gttacgcact ctgccaccct cacagatgga cag
//
LOCUS       DAZFLD010000339         2913 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.341, whole genome shotgun sequence.
ACCESSION   DAZFLD010000339 DAZFLD010000000
VERSION     DAZFLD010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2913)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2913
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.341"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>2913
                     /locus_tag="ACHKBL_004735"
     CDS             <1..>2913
                     /locus_tag="ACHKBL_004735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LPD7 domain-containing protein"
                     /protein_id="HFO5821473.1"
                     /translation="KPHVHIIIPEVNLLSGNLINPRGDYRVNEKYFEAVQEYINQKYN
                     LISPRERVRANPKNVSDILSRYKGDDFRSKNREFKEQLVRDIVEKNILSRKDFYTHVS
                     SFGDIRIRNQGKENEYIAVKLPGDKKFTNLKETIFNDDFIVRRELKKPPLEKSVIQQL
                     LHEWPQRAMELKYIDKANSDFRKKYYSSSQEEKIKILAQQETAFYQKYGERYDVFPAE
                     WKTNHKSGINEAQTRGTTFSPNGLQSMSGGEMATGWQTGNTGSSLFLSTDALVFMGQS
                     QSSGRDSGLRCDVHSGGRGRSGNQRGSGQPAITGTNATVHSRRSTRTQYGNRYAGGLL
                     AGDVSIPVYARNKPGGATIQDVIRRSEILLRPPPGKSNISVNRFNGDIPQMGKNAAYV
                     AAHFVRQYEQNKILPNQRQALWDIRQLYQSTRSFIWQDERLTRKERSQYLSVLAFEHV
                     KARQKIINSSQQENTMASEDIRKLIKKKPRLSNNEITGKDERKIPPAGKRFSRIVHTL
                     KNRIEGEDKENSYAKSVSAADLYTRKSKLSKNVHYLDKNSNRTLFVDTGNAIAIRKDG
                     MSPAALGIALELAKERFGSTLTIKGNEEFRNQVIDIVARNHPDLHFSDKAMNRQLEER
                     RAEIAAERDGMTIEGVSKTSTSKEQEKEHEQAADTKNQTNVKESSEPHESPESRTVVK
                     LEGKLIDHGKAPLDNKKGAEQSYFVTVRGRDGKETTHWDSDLQNVMKGKRRGTEISLE
                     LKESNPVQVSTRDHLGNLSIREAVRQTWTLNKMSVGQEQKREQSLEKIQQNQSPIPAR
                     TQTAVDLILANPVLRQMAMTAEQKEREFTALQADKESVREQTDQEIRNRLGSGDQSRE
                     PLAVILSDDELRGMALSMEQKMREMATPVDKLDAVKAEINSEIAARVARNSSIPVPVS
                     EVSATVAQKEIHREAESRGNKDKVHEGVLLDHGRAAYKFKPDISKPEDERDDSYFI"
BASE COUNT         1007 a          526 c          700 g          680 t
ORIGIN      
        1 gaaaaccaca tgttcatatt attattccag aggttaatct tctttctggt aatttaataa
       61 accccagggg agattacagg gtaaatgaaa aatattttga ggctgttcag gaatacataa
      121 accaaaaata taatctgatt tctccccgag agcgtgtcag agccaatcca aagaatgtgt
      181 ctgatatttt gtctcgttat aagggggatg atttcagaag taagaacaga gaatttaaag
      241 aacaactggt cagggatatt gttgaaaaaa atattttatc aagaaaagat ttttatacac
      301 atgtatcttc atttggtgat atccgaattc gtaatcaggg taaagaaaat gaatatatcg
      361 ccgttaaatt accgggtgat aaaaagttta ccaacctgaa ggagacaata tttaatgatg
      421 actttattgt gagacgagaa ctgaagaaac ctccactgga aaaatctgtc attcagcagc
      481 ttttgcatga atggccacag cgagctatgg agcttaagta cattgataag gctaacagtg
      541 atttccgaaa aaaatattat tcatcctcgc aggaggagaa aataaaaatt cttgcacaac
      601 aagaaacagc attttatcag aagtacggag aaagatatga cgtattccct gccgaatgga
      661 aaacaaatca taagtccggt attaatgaag ctcagaccag aggaactacc ttctctccca
      721 atggcttgca aagtatgtcc ggtggcgaaa tggcaactgg ttggcaaact ggaaacaccg
      781 ggagttcgtt gtttctgtcc actgatgcac ttgttttcat gggacagtcg caatccagcg
      841 gaagagattc tggactgcga tgcgatgtac attcaggagg aagaggacga tctggtaatc
      901 aacgaggaag cggacaaccc gctattaccg ggacgaatgc caccgttcac tccagacggt
      961 caacaagaac tcagtacgga aatcgatatg ccggaggatt actggccggg gatgtgagta
     1021 ttcctgtgta tgcgcgtaat aaacccggag gggcaacaat acaggatgta attcgccgta
     1081 gtgaaattct tttacgacca cctccaggaa aatcaaatat cagtgtgaac aggtttaatg
     1141 gtgatatacc ccaaatgggg aaaaacgcag cttatgtagc cgctcatttt gttcgtcaat
     1201 atgaacaaaa taagatatta cctaatcaac gtcaggcatt gtgggacatt cggcagctct
     1261 atcagtcaac ccggagtttt atctggcagg atgaacgatt aactaggaaa gaacgctcac
     1321 agtatctgtc agtactcgct tttgagcatg taaaagcgcg tcagaaaatc attaatagta
     1381 gtcagcagga gaatactatg gcaagtgagg atatccgcaa acttattaag aaaaaaccac
     1441 gcctgtcaaa taacgaaatt acagggaaag acgagcggaa gataccaccg gcaggaaaac
     1501 gtttttcaag aatagttcat acactgaaaa accgaattga aggtgaagat aaagagaatt
     1561 catatgcaaa gtctgttagt gcagcggatt tatataccag aaaatcgaaa ctcagtaaga
     1621 atgtgcatta tcttgataaa aacagtaaca ggacattatt cgttgataca ggaaatgcca
     1681 ttgctataag aaaagatggt atgtctccgg cggcgctggg gattgctctt gaactggcca
     1741 aagaacgttt tggcagtaca ctgactatca aggggaatga agaatttcgt aaccaggtta
     1801 ttgatattgt cgccagaaat cacccagatc ttcatttttc tgacaaagcg atgaatcggc
     1861 aacttgaaga acgacgggca gaaattgccg ctgaacgtga tgggatgaca atagaaggcg
     1921 tttctaaaac gagtacgagt aaggaacaag agaaagagca tgaacaggca gcagatacta
     1981 aaaatcaaac aaatgtgaag gagtcctcag agccacacga atcacctgaa agcaggacag
     2041 tagttaagct ggagggtaaa ttaatagatc acggcaaagc gcctcttgat aacaagaagg
     2101 gtgctgaaca aagttatttt gttacggtca ggggcaggga cggtaaagaa accacacact
     2161 gggatagtga tttgcaaaac gtaatgaagg ggaaacgccg gggaacagag atatctcttg
     2221 agttgaaaga atctaatccg gttcaggtca gtacacgcga tcatctcggc aatttgtcta
     2281 tcagggaagc ggtccggcag acttggacat taaacaagat gtcggtggga caggagcaga
     2341 aaagggaaca gtcgctggag aaaatacagc aaaatcaaag ccccatacca gccagaacgc
     2401 agacagctgt tgaccttatt cttgctaatc cagtattacg gcagatggct atgactgcgg
     2461 aacaaaagga aagagaattt actgcattgc aggcagataa agaatccgtc agagaacaga
     2521 ctgatcagga aataagaaat cgtctcggta gtggagatca aagcagggaa ccgttagccg
     2581 tcattttgtc agatgatgaa ctacgtggaa tggcgttatc gatggagcaa aaaatgcggg
     2641 aaatggctac tccagtagat aaattggatg cagttaaagc agaaattaac agtgaaattg
     2701 cagcgagggt tgcgcggaac agcagtattc ctgtgcctgt atcagaagtt agcgccactg
     2761 tggcacaaaa agaaatccat cgggaagcag agtcgagagg taataaagat aaagtacatg
     2821 aaggtgtgct acttgatcat ggccgggcag catacaaatt taagcctgat ataagcaaac
     2881 cagaagatga acgtgatgat agctatttta ttt
//
LOCUS       DAZFLD010000340         2896 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.342, whole genome shotgun sequence.
ACCESSION   DAZFLD010000340 DAZFLD010000000
VERSION     DAZFLD010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2896
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.342"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            2..1369
                     /locus_tag="ACHKBL_004736"
     CDS             2..1369
                     /locus_tag="ACHKBL_004736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001526835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HFO5821474.1"
                     /translation="MEKDYLGISSTVLVSLLFGLALVLVNSWFNQPTVEEVVPRSTYL
                     MVMIALFFIDTVAFIFMQLYFIYDRRQFSNCILSLAFLSCLIYFIKTVIIIQQIIEGR
                     LTSSVVQNDIAIYYLFRQMSLCVLIFLALVNKVSENTKQRNLFSKKVTLCITLFFVLG
                     GPIVAHIFSSHYESYDLHIAELTNKNGQVVWKASYVTIMIFMWLTLLSVNLYFNGLRC
                     DIWNGVSVIAFCAVLYNISLLFMSRYSVSIWYISRTIEVVSKLTVLVIFMCHIFSALR
                     VTKDIAHRDPLTNIFNRNYFFNELTVQSASAKKKPYCVMIMDIDHFKKVNDTWGHPVG
                     DQVIKTVVNIIGKSIRPDDLLARVGGEEFGVLLTEIDTECAKALAERIRENVERLTGD
                     NPEYAIPQKVTISIGAVVTQKNELNPKEIYQLADNALYEAKETGRNKVVVKEAEILIN
                     RKDDE"
     gene            1646..2854
                     /locus_tag="ACHKBL_004737"
     CDS             1646..2854
                     /locus_tag="ACHKBL_004737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000279862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO5821475.1"
                     /translation="MAVLTDTKARHIKPDDKPLPHGGITGLTLHPSSVKGRGKWVFRY
                     VSPVTQKRRNAGLGTYPEVSIAEAARTARIMREQLAAGDDPLEIKKAEAEKVVIPTFA
                     DAARRVHAELSPGWENPKHVRQWLSTLENYVFPQLGAKTLDTITAADVAETLRPVWLT
                     LSETASRVKQRIHVVMQWGWAHGFCVANPVDVVDHLLPQQSRGRDEHQPAMPWRQLPL
                     FVATSVYTDEPYNVTRALLLMVILTATRSGEARGMRWAEIDFHKRIWTIPAERMKARI
                     QHRVPLSRQAIHVLENIRGLHDELVFPSPRKQQILSDMVLTSFLRKKKAISDIPGRVA
                     TAHGFRSTFRDWCSEQGYSRDLAERALAHTLKNKVEAAYHRTDLLDQRIPMMQAWADY
                     VMSQIMENQR"
BASE COUNT          806 a          536 c          714 g          840 t
ORIGIN      
        1 tatggaaaaa gactatttgg gaattagtag tactgtatta gtgagcttat tgtttgggct
       61 tgcattggtt ttggttaata gttggttcaa tcagccaacc gttgaggaag ttgttccacg
      121 atcaacctat ctgatggtta tgattgcttt attttttatc gatactgttg catttatttt
      181 tatgcagttg tatttcattt atgaccgtag gcaattttca aactgtatac ttagtttggc
      241 ttttctaagt tgtttgattt actttattaa aaccgtcatt atcattcagc aaattattga
      301 ggggcgtttg acaagcagtg ttgttcaaaa tgacattgca atttattatt tgtttcgtca
      361 gatgagtttg tgcgtattaa tatttctggc attggtaaat aaagttagtg aaaacacaaa
      421 acagcgcaat ttgttttcca aaaaagtgac tttgtgcata actttgtttt ttgttcttgg
      481 ggggccgata gttgctcaca tattttctag ccattatgag agctacgatt tacacattgc
      541 cgagttaacc aataaaaatg gtcaggtggt ttggaaagcc tcatatgtta ccataatgat
      601 tttcatgtgg ttaaccttgc tttcggttaa tctatacttt aatggtttac gctgtgatat
      661 ctggaacggg gtgtcggtga ttgctttttg tgccgtgctg tacaatatct ctctgttatt
      721 tatgagcagg tatagcgtgt ccatttggta tattagtcgc actattgaag tagtcagtaa
      781 gttaaccgtt ttggtaattt ttatgtgcca tatattcagt gcgctacgag taacaaagga
      841 cattgcacat cgcgatccct taaccaatat atttaacaga aattattttt ttaatgaact
      901 gacggttcaa tcagcatcgg ccaaaaaaaa accttattgc gtcatgatta tggatattga
      961 ccacttcaaa aaagtcaacg acacctgggg acatccggtt ggcgatcaag tgataaagac
     1021 agtggtgaat atcattggga aaagcatacg accagatgac cttttagcgc gcgttggcgg
     1081 cgaagagttt ggcgtcttgc tgacggagat cgatactgaa tgtgcgaaag ctttagcgga
     1141 aaggattcgg gaaaatgttg aacgtttaac tggcgataat cctgaatacg ccataccaca
     1201 aaaagtgaca attagtattg gcgctgttgt aacgcagaaa aatgagttaa accctaagga
     1261 gatctatcaa ctggctgata acgcacttta cgaggcgaaa gagaccgggc gtaataaggt
     1321 ggtagtaaaa gaggcagaaa ttcttatcaa ccgcaaggat gatgaatgag tctgtcgcta
     1381 gttgtcgacc tccgcctggg gcttctcatc ccccagtgtg tgaaatatac gaaaaaaagc
     1441 tcgcactttc gtacgagctc ttctttaaat atggcggtga gggggaccgc ctgaaataaa
     1501 tctgacttaa tccgggagtg ttatataact tcattataaa caatgcatta caatttttgc
     1561 tgtccgatgt tatctaatgt attcttagtg aagtgagaga ctaatggcat actggatggc
     1621 ataccagagt aacaggaatt agcgtatggc agtattgacg gatactaaag caaggcatat
     1681 caaacctgat gacaaaccat tgccccatgg gggaattaca ggactgactc ttcatccttc
     1741 ttcagtaaag gggcggggga aatgggtttt tcgttatgta agtccggtga cacaaaaaag
     1801 gcgtaatgct ggattgggta cttaccctga ggtcagtatt gctgaagctg cacgtactgc
     1861 ccggataatg cgagagcaac ttgctgcagg tgatgatcct ctggagatta aaaaggctga
     1921 agctgaaaaa gttgttatcc caacatttgc cgatgcagcc cggcgtgtac atgcagaatt
     1981 gtcaccggga tgggaaaacc caaagcatgt caggcagtgg ttgtcgacgc ttgaaaatta
     2041 cgtgtttcct caactgggag caaaaacgct ggatacgatt acggctgcgg acgtggcaga
     2101 gacactgcgt ccagtctggt tgaccttatc agaaacggca agccgggtta aacagcgcat
     2161 tcatgttgtt atgcagtggg gctgggcgca tggtttttgt gtggcgaatc ctgttgatgt
     2221 ggttgatcat ttgcttccac agcaatcaag aggacgtgat gaacaccagc cggcaatgcc
     2281 ctggaggcag ttaccgcttt ttgtggcgac cagtgtgtat acagatgaac cttataatgt
     2341 tacccgggca ctgttattaa tggtgatact gacagcaacc cgctcgggcg aagcaagggg
     2401 aatgcgctgg gctgaaattg attttcataa gcggatatgg acgatacccg cagaaagaat
     2461 gaaagccagg atacagcatc gtgttccttt atcccgacag gccattcacg ttctggaaaa
     2521 tatacgtggt ctgcatgacg aactggtgtt tccttctccc agaaagcagc agatcctttc
     2581 agatatggtg ttgacgagtt ttctacgtaa aaagaaggcc atcagtgata tacccggacg
     2641 agtggctaca gcacatggtt ttcgttcaac attcagggac tggtgtagcg aacagggata
     2701 ttcgcgggat ttggcggaaa gggcgcttgc ccacacgctg aaaaataagg ttgaggcggc
     2761 atatcaccgg actgatctgc tggatcagcg tataccgatg atgcaggcat gggcggatta
     2821 tgtgatgtct cagattatgg aaaaccagcg atgaaatcac atctcacaga tttgcagatg
     2881 tcgtctgtaa tcacga
//
LOCUS       DAZFLD010000341         2892 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.343, whole genome shotgun sequence.
ACCESSION   DAZFLD010000341 DAZFLD010000000
VERSION     DAZFLD010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2892)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2892
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.343"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1938
                     /locus_tag="ACHKBL_004738"
     CDS             <1..1938
                     /locus_tag="ACHKBL_004738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001555799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent
                     hemoglobin/transferrin/lactoferrin family receptor"
                     /protein_id="HFO5821476.1"
                     /translation="TLIILPVGLQAATSHSSMAKDTITVVATGNQNTVFETPSMVSVI
                     TNDTPWSQNAVTSAGMLKGVAGLSQTGAGRTNGQTFNLRGYDKSGVLVLVDGVRQLSD
                     MAKSSGTYLDPALIKRIEVVRGPNSSLYGSGGLGGVVDFRTADAADFLPPGETNGVSL
                     WGNIASGDHSTGSGLTWFGKTGKTDALLSVIMRKRGNIYQSDGERAPNKEKPAALFAK
                     GSVSITDSNKAGASLRLYQNNTTEPGNPTLTHGDNGLRDRKTAQNDVQFWYQYSPADN
                     SLINVKSTLYLSDITIKTNGHNKTAEWRNNRTSGVNVVNRSHTLIFPGAHQLSYGAEY
                     YRQQQKPEGSATLYPEGNIDFTSLYFQDEMTMKSNPVNIIVGSRYDRYKSFNPRAGKL
                     KAERLSPRAAISVSPTDWLMMYGSISSAFRAPTMAEMYRDDVHFYRKGKPNYWVPNLN
                     LKPENNITREIGAGIQLDGLLTDNDRLQLKGGYFGTDARNYIATRVDMKRMRSYSYNV
                     SRARIWGWDMQGNYQSDYFDWMLSYNRTESMDASSREWLGSGNPDTLISDISIPVGHG
                     GVSAGWRAELSAAATHVKKGDPHQAGYAVHSFSLSYKPVSVKSFEASVTLDNAFNKLA
                     MNGKGVPLSGRTVSLYTRYQW"
     gene            1957..2748
                     /locus_tag="ACHKBL_004739"
     CDS             1957..2748
                     /locus_tag="ACHKBL_004739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000977390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4198 domain-containing protein"
                     /protein_id="HFO5821477.1"
                     /translation="MMKNTGYILALCLTASEHALAHDVWITGKQAENNVTAEIGYGHN
                     FPSKGTIPDRRNFFENPRLYNGKEIITLKPTSTDYVYKTESAANGNSYVLSTKMKPGY
                     WSRTSSGWKPVSREGRNDVAYCEFVTKYAKSFIPGEQQMPAQLYQSPTGDELEIIPLS
                     DISRFGEDVKLKVLYKTSPLAGATLELDSVSYLKSSRHTHAAEHKHSAHKAELTFVSN
                     EDGIITVPSLHVGQWLAKVKNKKVFPDKSLCDETVDVATLSFSRN"
BASE COUNT          830 a          676 c          691 g          695 t
ORIGIN      
        1 acattaatca tacttccggt ggggttacag gcagcgacaa gtcattcttc tatggctaaa
       61 gatacaatca ccgttgtcgc gacaggaaat cagaacacgg tatttgaaac gccgtcgatg
      121 gtcagtgtca tcacgaatga cacgccatgg agtcagaatg cggtcacatc agccggtatg
      181 ctgaaaggtg ttgcgggtct cagccagact ggtgcaggac ggaccaatgg gcagaccttt
      241 aatctacgcg gctatgacaa aagcggggta cttgttcttg ttgacggcgt ccgtcaactc
      301 agtgacatgg caaaaagcag tggcacttat ctggatccgg cactcatcaa acgcatcgaa
      361 gttgtccgcg gaccaaactc cagtctgtac ggcagtggcg ggctgggggg ggtggtggac
      421 ttcagaactg ccgatgctgc agattttctt ccccccggag agacaaatgg tgtaagtctg
      481 tggggaaata tcgccagtgg tgaccacagc acaggctcgg ggctcacctg gttcggtaaa
      541 accggaaaaa cagatgcgct tctttctgtc attatgcgta aaagaggaaa tatctatcag
      601 agtgatggtg agcgtgcacc taacaaggaa aaacctgcag ccctgtttgc gaaaggctct
      661 gtcagtataa cagacagtaa caaagcaggt gccagcttgc gtctctacca gaataacacc
      721 actgaaccgg gcaatcccac tctgacacat ggtgacaacg gcctgcgtga cagaaaaaca
      781 gcacaaaatg acgtgcagtt ctggtaccag tactcccctg cggataacag cctcatcaat
      841 gtaaagtcaa cgttatatct cagtgatatc actatcaaga caaacggtca caacaaaaca
      901 gcagaatggc gaaacaacag aacctccggt gttaatgttg tcaacaggag ccatactctg
      961 atttttccgg gagcccatca gttaagttat ggcgccgaat attaccgtca gcagcagaag
     1021 ccggaaggct ctgccacact atatccggaa ggaaacattg actttacatc gttgtatttc
     1081 caggatgaaa tgacaatgaa aagcaacccg gttaacatta tcgtcggttc ccgttatgac
     1141 cggtacaaga gcttcaatcc ccgagccgga aaactgaaag ccgaacgcct gtcccccagg
     1201 gcggcgattt cagtctcacc aacagactgg ctgatgatgt acggctccat atcctctgca
     1261 ttccgtgccc ccacaatggc agaaatgtac agggatgatg tacattttta ccgcaagggt
     1321 aaacccaatt actgggtacc taaccttaat ctgaaaccag aaaataacat tacccgtgag
     1381 attggcgcag gtattcaact ggacggcctg cttacagaca atgaccggct gcagttgaaa
     1441 ggcggatatt tcggaacgga tgccagaaac tatattgcca cgcgcgtgga tatgaaacgg
     1501 atgcgttctt attcttataa tgtatcccgg gcccgtatct ggggatggga tatgcagggt
     1561 aattaccagt ctgattattt tgactggatg ctttcttata accgcacgga aagtatggat
     1621 gccagtagca gggaatggct gggctccggc aatcctgaca cactcatcag tgacatcagt
     1681 atacctgttg gtcatggagg agtttctgcc ggatggcgtg ctgaactttc ggcagcagcc
     1741 acgcatgtga aaaaaggcga tccccatcag gctggctatg ccgtacattc cttttcactg
     1801 tcttataagc ctgtaagtgt taaaagcttt gaggcatcag tgactctgga taatgccttc
     1861 aacaagcttg ccatgaatgg caaaggtgtg ccgctttcag gcagaactgt cagtctttat
     1921 acccgttatc agtggtaacc tcgcaggaaa taaatcatga tgaaaaatac aggctatatc
     1981 ttagctcttt gtctgacagc atcggagcat gccctggccc acgatgtctg gattaccggt
     2041 aaacaggcag agaacaacgt taccgcagag attggttatg gtcataattt cccctcaaag
     2101 ggcacaattc ctgacagaag gaatttcttt gaaaatcccc ggctttataa cgggaaagag
     2161 ataataacgc tgaagccaac gtccacggat tatgtctata aaactgagtc tgcagccaac
     2221 ggtaatagct acgttctgtc aacgaaaatg aaaccgggat actggtcgag aacctcgtca
     2281 ggatggaagc ctgtcagtcg ggagggcaga aatgatgtgg catactgtga gtttgtgact
     2341 aaatatgcaa aatcttttat tcctggtgaa cagcagatgc cagcacaact ctatcagtct
     2401 ccaacagggg atgagcttga aatcattcca ttatccgata tcagtcgttt cggtgaagat
     2461 gtgaagctga aagttctgta taaaacatcc ccgcttgccg gagctactct ggagcttgac
     2521 tctgtcagtt atctgaaatc atcccgtcat acccatgcag ctgagcataa acattcggct
     2581 cataaagcag aacttacctt tgtaagtaat gaggacggta tcatcactgt accttccctt
     2641 catgtcgggc aatggctggc taaagtcaaa aataagaaag tatttccgga caagagtctg
     2701 tgtgatgaaa ctgtcgatgt ggcaacctta agcttctccc gaaattaata atactattcc
     2761 tgccctgatt tatgggcagg gtattatcaa tacgagaata gcctaccgac atccgtgtaa
     2821 aaaaccacat aatgattttt catatccatt tccagatata cttatgtaaa gatcacctcc
     2881 aaaaaagcgt aa
//
LOCUS       DAZFLD010000342         2834 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.344, whole genome shotgun sequence.
ACCESSION   DAZFLD010000342 DAZFLD010000000
VERSION     DAZFLD010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2834
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.344"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..226
                     /locus_tag="ACHKBL_004740"
     CDS             <1..226
                     /locus_tag="ACHKBL_004740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001210963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HFO5821478.1"
                     /translation="RAFMSVEKLTLSGRQVNVLMMLLYGSNGTEGARYLGMSEKTFST
                     HKQNLIKRLRVHNEVELLYKGMLLVRKGRL"
     gene            301..489
                     /locus_tag="ACHKBL_004741"
     CDS             301..489
                     /locus_tag="ACHKBL_004741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HFO5821479.1"
                     /translation="MRLLIAFLLPWLTFFTIGRPLTGIFCLVLQITLLGWIPATIWAV
                     YALSQYKTEQKIREATGK"
     gene            633..1478
                     /gene="mscS"
                     /locus_tag="ACHKBL_004742"
     CDS             633..1478
                     /gene="mscS"
                     /locus_tag="ACHKBL_004742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000340087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HFO5821480.1"
                     /translation="MVRNQDMLPGYAANLVAAVVIIIGGIAARMISGVLNNLMLGRNI
                     DATVALFLSALIRHAIIAFTLIAALGRVGVQTASVIAVLGAAGLAVGLALQGSLSNLA
                     AGILLVMFRPFRAGEYADLGGVAGTVQNVHIFSTTMRTLDGKIVVIPNGKIIAGEIVN
                     FSREPERRNEFIISVSYDADIDCVKQVLTDIVMSEERVLKNREITVRLNEPGESSMNF
                     VVRVWSRRDDLQSVYWDVLERIKREFDSEGISFPYPQMDIHLVRSGKQAESVTHNSRS
                     VSSRQ"
     gene            1604..2824
                     /locus_tag="ACHKBL_004743"
     CDS             1604..2824
                     /locus_tag="ACHKBL_004743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001124813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
                     /protein_id="HFO5821481.1"
                     /translation="MPKKIMTGIDVFDAAMERIEWVFDTFSSVCLSFSGGKDSTVLFH
                     LMANIARRKKRRFSVLFVDWEAQYQCTIEHVTRLREMYDDITDTFFWVALPLTTVNGV
                     SQVQPEWVCWEDGAEWVRQPPEDAITRASFFPFYQNAMTFEEFIPAFNHWFAREKGMV
                     AAILTGVRADESLNRFMGLVSGRKLRYAEDKPWTTASAEGFYYTMYPLYDWKTRDVWI
                     YHARTRAAYNSLYDLMYRAGVPLRNMRVCEPFGPEQRKGLWLYHVLEPDTWGRMCARV
                     SGAVGGALYANESGAYFALRKQITKPVHHTWQSYAMFLLESMPEKTAEHYKNKIAVYL
                     RWWQSHGWPDGIPDEQDNDQGCKDIPSWRRICKTLLKNDFWCRMLAFSPNKPRHYERY
                     LARMREKRKEWGIL"
BASE COUNT          701 a          536 c          789 g          808 t
ORIGIN      
        1 ccgcgcattc atgtcggtgg agaagctgac cctgtcaggc cggcaggtaa atgtactgat
       61 gatgctgctt tatggcagta acggaacaga gggggcacga tatcttggca tgtcagagaa
      121 aacattctcc actcataagc agaatctgat aaagaggtta cgggtacata atgaagttga
      181 gttgttgtac aaggggatgc ttctggtcag aaaagggcgg ttgtgaagca gggcagctta
      241 tttccggtaa tgttaaacgc agcctctgat tcgttaattt cataatcata aggagttttt
      301 atgcgtctgt tgattgcttt tcttcttccc tggctgactt ttttcaccat cggccggcct
      361 ctgacgggga ttttttgtct ggtgttacag ataacgctgc tgggctggat accggcaaca
      421 atatgggccg tgtatgcgct ttcgcagtat aagacggaac agaaaatccg cgaagccacg
      481 gggaaatgat gactgcagaa gagcaggttg tcataccggt gtatatgatg cgggataaag
      541 gcccggagat tcctggaaat aaaatgttcc ggggaatata aaacagggaa aaaccggatg
      601 tgatggacgc ttctggcgag gcggtttcat gaattgtccg taatcaggat atgttgcccg
      661 gttacgccgc gaatcttgtg gcagcagtgg ttattattat cgggggtatc gctgcacgga
      721 tgatttctgg tgtgctgaat aatctgatgc tgggcaggaa tattgatgcc acagtggcgc
      781 tgttcctttc cgcgctgata cgtcatgcca ttatagcctt tacgctgatt gctgccctgg
      841 gacgtgtggg ggttcagaca gcttccgtaa ttgcagtcct gggggctgca ggtcttgctg
      901 ttggacttgc cctgcagggg tcattgtcaa accttgcagc tggtattctt ctcgtgatgt
      961 tccgtccttt cagggctggt gaatatgctg acctgggtgg tgttgccggg actgtgcaga
     1021 atgtacatat attctcaacc acgatgcgta cactggatgg taaaattgtt gtcattccga
     1081 acgggaagat tattgccgga gagattgtta atttctccag ggagcctgaa cgacgaaatg
     1141 agtttatcat cagtgtttca tatgatgcag atattgactg tgtgaaacaa gtcctgacag
     1201 atattgtgat gtcagaagag agggttctga agaacaggga aatcactgtt cgcctgaatg
     1261 agcctgggga gtcatccatg aattttgtag tacgggtatg gagcaggcgt gatgaccttc
     1321 agtctgtata ctgggatgta ctggagagaa ttaagcggga gtttgattca gaagggataa
     1381 gttttccata tccacaaatg gatatccatc ttgttcgctc cgggaagcag gcagagtcgg
     1441 taacccataa cagtcgctca gtcagttcca gacagtaaaa tgaaaagcgc attttatttg
     1501 tgaaagatga acagcctgaa tctgtcaggc tgttttcatt gtgtttttat ggtcattttt
     1561 ggtatatgta ctgtatataa tgtcatctgt acaaggatat aaaatgccaa agaaaataat
     1621 gacagggatt gatgtttttg acgctgccat ggagcgtatt gaatgggtgt ttgatacgtt
     1681 ttcatcagtt tgtctttctt tttccggcgg aaaagactcc actgtcctgt ttcacctgat
     1741 ggcgaatatt gcccgccgaa aaaaacgccg tttttctgta ctgtttgttg actgggaggc
     1801 tcagtatcag tgtaccattg agcatgtcac caggttgagg gaaatgtacg atgatatcac
     1861 agatactttt ttctgggtgg cgctgcctct gacgaccgtt aatggagtgt ctcaggtaca
     1921 gcctgagtgg gtatgttggg aggacggggc tgaatgggtt cgccagccac cagaagatgc
     1981 cataaccaga gcgtcttttt ttccttttta tcagaatgcg atgacatttg aagagtttat
     2041 tccggcattt aatcattggt ttgcccggga aaaagggatg gttgctgcga ttcttactgg
     2101 tgttcgcgct gatgagtcac tgaatcgttt tatgggactg gtatccggac gtaagctccg
     2161 ttatgcggag gataaaccct ggaccacggc atcggctgag ggattttact acaccatgta
     2221 tcctttgtac gactggaaaa caagagatgt atggatttac catgcgagaa cacgtgctgc
     2281 ctataactct ctttatgacc tgatgtacag ggctggagtt cctcttcgca atatgcgggt
     2341 gtgtgagcct tttggaccgg agcaacgtaa ggggttgtgg ctttatcatg tactggagcc
     2401 ggatacatgg ggcagaatgt gtgctcgcgt atcaggtgct gtagggggag cgctctatgc
     2461 aaatgaaagt ggtgcttatt ttgctttgag gaaacagata acaaaaccag ttcaccatac
     2521 atggcagagt tatgcaatgt ttctgctgga gtcaatgcct gagaaaacgg cggagcatta
     2581 caagaataag attgctgttt atcttcggtg gtggcagtct catggatggc cggatggcat
     2641 ccctgatgag caggataatg accagggatg taaagatatt ccctcctggc gtcgcatctg
     2701 taagacgtta ctgaaaaatg atttctggtg tcggatgctg gcgtttagtc cgaataagcc
     2761 ccgacattat gagcgctacc tggccagaat gcgtgaaaaa aggaaagagt ggggaatatt
     2821 gtaaattata ttta
//
LOCUS       DAZFLD010000343         2821 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.345, whole genome shotgun sequence.
ACCESSION   DAZFLD010000343 DAZFLD010000000
VERSION     DAZFLD010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2821
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.345"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..229
                     /locus_tag="ACHKBL_004744"
     CDS             <1..229
                     /locus_tag="ACHKBL_004744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TIGR03750 family conjugal transfer protein"
                     /protein_id="HFO5821482.1"
                     /translation="PLLGWVIIPTGMLLTPLLLVWFGGVWISRLKRGKPDNWLWQHLA
                     KKSSAWGGGRKTFIREAHGWSLRRTRSRGKK"
     gene            226..888
                     /locus_tag="ACHKBL_004745"
     CDS             226..888
                     /locus_tag="ACHKBL_004745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001422135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PFL_4703 family integrating conjugative element
                     protein"
                     /protein_id="HFO5821483.1"
                     /translation="MSRFRHALRDRDQHILTLRIFSGVLAGILMLVCIGWMTAPDRLT
                     IHNPPDLRSGSTRPWWEVPPSTVYSFAFYIFQQLNAWPKNGEVDYPAKIHALSAYLTP
                     SCREILESDARRRSDAGELLERVRVVYEIPEHGYGGRYNKNPVTVEGRDNWQVQLDLV
                     ADEYFHAEPVKRALVRYPIKVVRMEGDPERNPFGLALDCYSSTPQRLEAASPAAGKGD
                     NQ"
     gene            888..1796
                     /locus_tag="ACHKBL_004746"
     CDS             888..1796
                     /locus_tag="ACHKBL_004746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03749 family integrating conjugative element
                     protein"
                     /protein_id="HFO5821484.1"
                     /translation="MNGAERCLYRSIVMVLSLLVLPLSARADELMKWERIPLPVTLMA
                     GQERVIFADKNVRVGFPPALNGKLRVQSTGGAVYLKAESVFPQTRLQLQDVESGEIIL
                     LDVTAAEKGPTEPVRIVYEGSVSTVSQAGTAASARSAEHKKVVPGGADGSQALRQPVK
                     YAEPLPVLLTRYAAQSLYAPLRTLEAVPGIRPVNTHLPGKITTLYPSEPLTVTPLAAW
                     AAGGRAVVALKLTSTVSRKIILDPRALQGKFIAATFQHRWLGAAGTPEDTTTLYVVTA
                     ERPEQAFIAEPASRRIPSRGEVKHAD"
     gene            1786..>2821
                     /locus_tag="ACHKBL_004747"
     CDS             1786..>2821
                     /locus_tag="ACHKBL_004747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001506261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03752 family integrating conjugative element
                     protein"
                     /protein_id="HFO5821485.1"
                     /translation="MQIKSNTIVKYVVPCVVVAGILTGLKSCGGDTGEPSSAESSGTL
                     AGLSPDELKALGVEGDTPEDTLRTLIGRMNQVRERQLVLDKQNEKLLKENEMLRKRGT
                     DVSGQVSEAVAGLQKNYEQSQRQFRNEQNALMTQVQSLTEQLKRQQESADAGAGGQES
                     DIPAGLGLDGLGSVTGSAASPGTDGLMWVTPADQVQDEASSVGDKSGPRFPVSFLDDN
                     PLTRQKAAYEQQVKGYSNEQQKASTRAVYTLPENSTLAGSRAMTALLGRVPVNGTVTD
                     PYPFKILVGKDNLTANGIELPDVEGAVVSGTASGDWTLSCVRGQVNSITFVFADGTVR
                     TLPSSDVNGSS"
BASE COUNT          635 a          687 c          932 g          567 t
ORIGIN      
        1 tcctctactt ggctgggtga ttatcccgac agggatgttg ctgactcccc tgctgctggt
       61 ctggttcggt ggcgtctgga tcagccggct gaagcgggga aaacctgaca actggctctg
      121 gcagcatctg gcgaagaaaa gcagtgcatg gggtggtggc cggaaaacat ttatccggga
      181 ggcgcatggc tggtccctgc gccgtacccg tagcaggggg aaaaaatgag tcgttttcgt
      241 catgctctgc gggatcgcga ccagcatatc ctgacactgc gtattttcag cggtgtgctg
      301 gccggaattc tgatgctggt ctgtatcggg tggatgacgg caccggacag actgacaatt
      361 cacaatccgc cggatctgcg ttccggcagt acccgtccct ggtgggaagt cccgccgtca
      421 acggtttatt cctttgcctt ttatatcttt cagcaactga atgcctggcc gaagaacgga
      481 gaggtggact acccggcgaa aattcatgcg ctctccgcct acctgacgcc atcctgtcgg
      541 gagattctgg agagtgatgc ccgccgtcgc agtgatgcgg gggagctgct ggagcgtgtg
      601 cgcgtggtgt atgaaattcc ggaacatggc tacgggggca ggtacaacaa aaatccggtg
      661 acggtggaag ggcgggataa ctggcaggta cagctggatc tggtggcaga tgagtatttt
      721 catgctgagc cggtaaaacg ggcacttgtc cgttatccga taaaggtggt gcgcatggag
      781 ggggatcctg aacgtaatcc gttcgggctg gcgctggact gctacagcag tacaccacag
      841 cggctggaag cagcctcccc ggcagccggg aagggggata atcagtgatg aacggagctg
      901 aacgttgttt ataccgtagt attgtgatgg tcctgtcact gctggtttta ccactgagcg
      961 cacgggctga tgagctgatg aaatgggagc gcattccctt acccgttacc ctgatggcag
     1021 gacaggagcg ggttattttt gcagataaaa atgtgcgggt gggcttcccg ccagcactga
     1081 acggaaagtt gagggtacag agtaccggtg gggcagtcta tctgaaagca gagagtgttt
     1141 ttccgcagac gcgtctgcaa ctgcaggatg tggagagcgg agaaattatt ctgctggatg
     1201 tgacagcagc agaaaaaggg cccacagagc ccgtacggat tgtgtatgag ggaagcgtca
     1261 gtacggtcag tcaggctggc acagcggcgt ctgcacgctc tgcagaacac aaaaaggtgg
     1321 tacccggcgg ggctgacggc tcgcaggccc tccgtcagcc ggtgaagtat gcagagcctt
     1381 tacccgttct gttgacccgc tatgcggcac agtcgctgta cgcaccgtta cggacgctgg
     1441 aagcggtacc gggtatccgg ccggttaata cacatctgcc cgggaaaatc accacactct
     1501 atccgtcaga gccactgaca gtcacgccac tggcagcctg ggctgcgggt gggcgtgcag
     1561 ttgttgcact gaaactgacg agtacggtaa gccgcaaaat tatccttgat ccgcgggcac
     1621 ttcagggaaa attcattgcc gcgactttcc agcaccgctg gctgggtgca gcaggtaccc
     1681 ctgaggatac caccacgctc tatgtggtga cggcagaacg gccggaacag gcgtttatcg
     1741 cggagcctgc ctcccgtcgc attccttcac ggggggaggt gaagcatgca gattaaatcc
     1801 aatacgatcg tgaagtatgt ggtgccctgt gtggtggtgg cggggatcct gacgggatta
     1861 aaaagctgtg gaggagatac cggggagccc tcgtctgcgg aaagctcagg aacgctggcc
     1921 ggactgtcac cggatgaact gaaggccctg ggcgtggaag gagacacacc tgaagatacc
     1981 ctgcgcacac tgattgggcg tatgaatcag gtgagggagc ggcaactggt actggacaaa
     2041 cagaatgaaa agttgctgaa agaaaatgaa atgctgcgca agcggggcac ggatgtttcc
     2101 ggtcaggtca gtgaggcagt ggcaggattg cagaaaaatt atgagcagtc gcagcgccag
     2161 ttccggaatg aacagaacgc gttaatgaca caggttcagt cactgacaga gcaactgaag
     2221 cgtcagcagg agagtgctga tgccggcgcc ggaggacagg agagcgatat tccagcaggt
     2281 ctgggccttg acggactggg gagcgtgaca ggatcggcag cctcaccggg aacggatggt
     2341 ctgatgtggg tgacaccggc cgatcaggtg caggatgaag cgtcgtcggt gggggataag
     2401 agcggaccgc gttttccagt ctcttttctg gatgacaatc cactgacccg tcagaaagcg
     2461 gcttatgaac agcaggtgaa gggatacagc aatgaacagc agaaggcgag cacccgggca
     2521 gtgtataccc tgccggagaa ctcgacgctg gcaggcagcc gggcgatgac agcgcttctg
     2581 ggacgggttc cggtgaacgg aaccgtgacg gatccctatc cgttcaagat cctggtgggt
     2641 aaggataacc tgacggcaaa cggaatagag ctgccggatg tggaaggtgc agtggtgtcc
     2701 ggtactgcga gtggtgactg gacgctctcc tgtgtccgtg ggcaggtgaa cagcatcacg
     2761 tttgtttttg ccgacgggac agtgcgtacg ctgccgtcat cagacgtgaa cggcagcagt
     2821 a
//
LOCUS       DAZFLD010000344         2810 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.346, whole genome shotgun sequence.
ACCESSION   DAZFLD010000344 DAZFLD010000000
VERSION     DAZFLD010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2810
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.346"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(58..2760)
                     /gene="chiA"
                     /locus_tag="ACHKBL_004748"
     CDS             complement(58..2760)
                     /gene="chiA"
                     /locus_tag="ACHKBL_004748"
                     /EC_number="3.2.1.14"
                     /EC_number="3.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chitinase/lysozyme"
                     /protein_id="HFO5821486.1"
                     /translation="MKLNIFTKSMIGMGLVCSALPALAMEVWNNQQGGNKYQVIFDGK
                     IYENAWWVSSTNCPGKAKANDATNPWRLKRTATAAEISQFGNTLSCEKSGSSSSSNSN
                     TPASNTPANGGSATPAQGTVPSNSSVVAWNKQQGGQTWYVVFNGAVYKNAWWVASSNC
                     PGDAKGNDASNPWRYVRAATATEISETSNPQSCTSAPQPAPDVKPAPDVKPAPDVQPA
                     PADKSNDNYAVVAWKGQEGSSTWYVIYNGGIYKNAWWVGAANCPGDAKENDASNPWRY
                     VRAATATEISQYGNPGSCSVKPDNNSGAVTPVDPTPETPVTPVTPTPDNNEPSTPADS
                     SNDYSLQAWSGQEGSEIYHVIFNGNVYQNAWWVGSEDCPRGTSAENSNNPWRLVRTAT
                     AAEMSQYGNPTTCEIDNGGVIVADGFQASKAYSANSIVDYNDAHYKTSVDQDAWGFVP
                     GGDNPWKKYEPAKAWSASTVYVKGDRVVVDGQAYEALFWTQSDNPALVANQNATGSNS
                     RPWKPLGKAQSYSNEELNNAPQFNPETLYASDTLIRFNGANYISQSKVQKVSPSDSNP
                     WRVFVDWTGTKERVGTPKKAWPKHVYAPYVDFTLNTIPDLAALAKNHNVNHFTLAFVV
                     SKDANTCLPTWGTAYGMQNYAQYSKIKALREAGGDVMLSIGGANNAPLAASCKNVDDL
                     MQHYYDIVDNLNLRVLDFDIEGTWVADQASIERRNLAVKKVQDKWKSEGKDIAIWYTL
                     PILPTGLTPEGMNVLSDAKAKGVELAGVNVMTMDYGNAICQSANTEGQNIHGKCATSA
                     IANLHSQLKGLHPNKSDAEIDAMMGTTPMVGVNDVQGEVFYLSDARLVMQDAQKRNLG
                     MVGIWSIARDLPGGTNLSPEFHGLTKEQAPKYAFSEIFAPFTKQ"
BASE COUNT          672 a          740 c          660 g          738 t
ORIGIN      
        1 ccaaaagtag cccattgaca aaaaatgcgg cgataccgga aggtatcgcc aacacattta
       61 ttgcttagta aacggcgcga agatttcgct aaatgcgtac ttcggtgcct gttctttagt
      121 caggccgtgg aattccggag acaggttagt gccgcctggc aggtcgcgcg cgattgacca
      181 gataccaacc ataccgagat tacgcttctg cgcatcctgc atgaccagac gagcatcaga
      241 gagatagaac acctcgccct gaacgtcgtt cacgccaacc atcggcgtgg tacccatcat
      301 agcgtcaatt tctgcatcgc tcttattggg atggaggcct ttcaattgtg aatgcaggtt
      361 ggcaatcgca gacgttgcac acttaccgtg aatgttctgg ccttcggtat ttgcagactg
      421 acaaatcgcg ttaccgtagt ccattgtcat cacgttcaca cccgccagct caacaccttt
      481 cgctttggca tcgctcagga cattcatccc ttccggcgtc aggccagtcg gcagaattgg
      541 caaggtgtac cagatagcaa tatctttgcc ctctgacttc catttatcct gcactttctt
      601 cacagcaagg ttacgacgtt caatagatgc ctgatccgca acccaggtgc cttcgatatc
      661 gaagtccagg actctgaggt tcaggttatc aacgatgtca taataatgct gcatcagatc
      721 gtctacgttc ttacaggaag cagccagcgg agcgttgtta gcaccaccga tagacagcat
      781 cacatcgccg ccagcttcac gcagagcttt gattttgctg tactgagcgt aattctgcat
      841 accgtaagcg gtaccccatg tcggcagaca ggtgttcgca tctttactca ccacaaacgc
      901 cagcgtgaag tggttgacgt tatgattctt agccagcgca gccagatccg ggatcgtatt
      961 cagcgtaaag tcaacatacg gtgcataaac gtgtttcggc cacgctttct tcggcgtacc
     1021 tacacgttct ttggttccgg tccagtcaac aaaaacacgc cacgggttgc tgtcagaagg
     1081 agaaactttc tgcactttac tctgagaaat gtagttcgca ccgttaaagc gaatcagcgt
     1141 atcgctggca taaagcgttt ctggattaaa ctgcggcgca ttattcagct cttcgttgct
     1201 atagctctga gccttaccta acggcttcca cgggcggcta ttgctaccgg tggcgttttg
     1261 gttcgcaacc agagctgggt tgtcgctttg cgtccagaac agcgcttcat aagcctgccc
     1321 atcaacaaca acgcgatcac ctttcacgta cacggtggaa gcggaccatg ctttcgccgg
     1381 ttcgtatttc ttccacgggt tatcgccgcc cgggacaaag ccccatgcgt cttgatcgac
     1441 agaggtttta taatgtgcat cgttataatc tacgatgctg ttcgcgctgt acgctttgct
     1501 ggcctggaaa ccatccgcaa caatgacgcc gccattatca atttcacagg tagttgggtt
     1561 accgtactga ctcatttccg cagcggtagc tgtacgcacg agacgccatg ggttattgga
     1621 gttttctgca ctagtgccac gtgggcaatc ttcagaacca acccaccagg cattctgata
     1681 aacattacca ttgaagataa catggtaaat ttcgctacct tcctggccac tccacgcctg
     1741 cagtgagtaa tcgttactgc tatccgctgg tgttgatggc tcgttattat ccggagtagg
     1801 agtaactgga gttaccggtg tttccggagt tggatcaacc ggagtcacag caccgctatt
     1861 attatccggc ttaacggaac aggaacccgg attaccatac tggctgattt ctgttgccgt
     1921 tgccgcgcga acataacgcc atgggttgct ggcatcgttt tctttcgcat cgcctgggca
     1981 atttgccgcg cctacccacc aggcgttctt ataaatgccg ccgttgtaga taacgtacca
     2041 tgtagaagaa ccttcctgac ctttccaggc tacgacagca tagttgtcgt ttgacttatc
     2101 agctggagct ggctgaacat caggagccgg tttaacgtcc ggtgccggtt tcacatccgg
     2161 cgcaggctgt ggtgctgaag tacaggactg tggattactg gtttctgaga tttccgttgc
     2221 cgtagcggca cgaacataac gccatgggtt gctggcatca ttgcctttcg catcacccgg
     2281 gcaattagaa gaggctaccc accaggcatt tttatatacc gcaccattaa agacgacata
     2341 ccaggtctga ccgccctgct gtttattcca ggcaactaca gaagaattag acggaacagt
     2401 gccctgtgct ggtgtagccg aaccgccatt agccggcgta ttggatgcag gcgtatttga
     2461 atttgaagaa gatgagctgc cgctcttttc gcaggaaagt gtattgccaa actgactaat
     2521 ttcagcagct gtcgcggtac gctttaaacg ccatgggtta gttgcatcat ttgcttttgc
     2581 ttttcccggg caatttgtag aagaaaccca ccaggcattt tcataaattt tgccatcgaa
     2641 aataacctga tatttattac caccttgttg gttattccat acttccattg ccaatgctgg
     2701 cagagcggaa cacaccagcc ccataccaat catagattta gtaaatatat ttaatttcat
     2761 tataatccct tgtgacgtaa aaactgcaaa acaaaagtcc tacgcaggaa
//
LOCUS       DAZFLD010000345         2803 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.347, whole genome shotgun sequence.
ACCESSION   DAZFLD010000345 DAZFLD010000000
VERSION     DAZFLD010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2803
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.347"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            43..2322
                     /gene="ydeP"
                     /locus_tag="ACHKBL_004749"
     CDS             43..2322
                     /gene="ydeP"
                     /locus_tag="ACHKBL_004749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance putative oxidoreductase YdeP"
                     /protein_id="HFO5821487.1"
                     /translation="MKKKIESYQGAAGGWGAVKSVANAVRKQMDIRQDVIAMFDMNKP
                     EGFDCPGCAWPDPKHSASFDICENGAKAIAWEVTDKQVNASFFAENTVQSLLTWGDHE
                     LEAAGRLTQPLKYDDVSDCYKPLSWQQAFDEIGERLQSYSDPNQVEFYTSGRTSNEAA
                     FLYQLFAREYGSNNFPDCSNMCHEPTSVGLAASIGVGKGTVLLEDFEKCDLVICIGHN
                     PGTNHPRMLTSLRALVKRGAKMIAINPLQERGLERFTAPQNPFEMLTNSETQLASAYY
                     NVRIGGDMALLKGMMRLLIERDDAASAAGRPSLLDDEFIQTHTIGFDELRRDVLNSEW
                     KDIERISGLSQTQIAELADAYAAAERTIICYGMGITQHEHGTQNVQQLVNLLLMKGNI
                     GKPGAGICPLRGHSNVQGDRTVGITEKPSAEFLARLGERYGFTPPHAPGHAAIASMQA
                     ICTGQARALICMGGNFALAMPDREASAVPLTQLDLTVHVATKLNRSHLLTARHSYILP
                     VLGRSEIDMQKSGAQAVTVEDSMSMIHASRGVLKPAGVMLKSECAVVAGIAQAALPQS
                     VVAWEYLVEDYDRIRNDIEAVLPEFADYNQRIRHPGGFHLINAAAERRWMTPSGKANF
                     ITSKGLLEDPSSAFNSKLVMATVRSHDQYNTTIYGMDDRYRGVFGQRDVVFMSAKQAK
                     ICRVKNGERVNLIALTPDGKRSSRRMDRLKVVIYPMADRSLVTYFPESNHMLTLDNHD
                     PLSGIPGYKSIPVELEPSN"
     gene            2570..2767
                     /gene="safA"
                     /locus_tag="ACHKBL_004750"
     CDS             2570..2767
                     /gene="safA"
                     /locus_tag="ACHKBL_004750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system connector SafA"
                     /protein_id="HFO5821488.1"
                     /translation="MHATTVKNKITQRDNYKEIMSVIVVVLLLTLTLIAIFSAIDQLG
                     ISEMGRIARDLTHFIINSLQD"
BASE COUNT          769 a          649 c          676 g          709 t
ORIGIN      
        1 tattacaaat cctaataatt catttccaca caggataagc agatgaagaa aaaaattgaa
       61 tcctaccagg gagctgccgg tggttggggt gctgttaaat ccgtagcaaa tgcagtacgt
      121 aagcagatgg atatacgcca ggatgttatt gccatgtttg atatgaataa gccagagggc
      181 tttgactgtc cgggatgtgc atggccagat cctaagcaca gtgcgtcatt cgacatttgt
      241 gaaaacggcg caaaagcaat cgcctgggaa gtcacggata agcaggtaaa tgcctctttc
      301 tttgctgaga atacggttca atcattactt acctggggag atcacgagct tgaggctgcg
      361 ggacgtctca ctcagccttt gaaatatgat gacgtcagcg actgttacaa gccattaagc
      421 tggcaacaag ctttcgacga aattggcgaa cgccttcaaa gctatagtga tcccaatcag
      481 gttgaattct atacttcggg ccgcacttcc aatgaagctg cctttcttta tcagcttttt
      541 gcccgtgaat acgggagcaa taacttcccc gactgctcca acatgtgcca tgaaccgacc
      601 agtgtgggtt tggcagcgag tatcggtgta ggtaaaggga ccgtgttgct ggaagacttt
      661 gagaaatgcg atttagtcat ttgcattggg cataaccctg gtacaaacca ccctcgcatg
      721 ttgacttcgt tgcgcgcttt agtgaaacgg ggagcgaaaa tgatcgccat caatcctcta
      781 caggaacgtg gcctggagcg atttaccgca ccgcaaaacc cgtttgaaat gctgacgaac
      841 tctgagactc agttggccag tgcctactac aacgtgcgca ttggtggtga tatggcgttg
      901 ctcaagggga tgatgcgcct gttaattgag cgcgatgatg ctgcaagcgc cgcaggtcgg
      961 ccctcattgc tggatgacga atttattcaa acgcatacca tcggctttga cgagctacgc
     1021 cgtgacgtgc tcaattccga gtggaaagat atcgaacgta tttctggact aagtcagaca
     1081 caaatcgccg aacttgctga tgcatatgcc gctgccgaac gaaccattat ctgttacgga
     1141 atggggatca ctcagcacga acatggtacc cagaacgtac agcaactggt caatctgctg
     1201 ttgatgaaag gtaacattgg taagcctggt gcgggtatct gcccactacg aggtcactct
     1261 aatgtacagg gcgaccgaac cgtcggtatc accgagaaac cgtctgcaga gtttctggct
     1321 cgcctgggtg agcgctatgg tttcacccca cctcatgcac ctggacatgc tgcaattgcc
     1381 agcatgcaag caatatgtac ggggcaggct cgagcattga tctgcatggg gggcaacttt
     1441 gcactggcaa tgccagatcg ggaagcaagc gctgtaccgt taacacaatt agatctgacg
     1501 gtacacgtag ccactaagct taaccgctct catctgctga ccgcacggca tagctatatt
     1561 ctgccggttc tgggacgcag cgagattgac atgcaaaaaa gcggtgcgca ggcggtaacc
     1621 gttgaggatt caatgtcgat gattcatgcc tcgcgtggcg tgttaaaacc cgccggtgta
     1681 atgctgaaat cagagtgtgc agtggtcgcg ggaatcgcgc aggcagcact accccagagc
     1741 gtggtagcct gggagtatct ggtggaagat tatgatcgca ttcgcaatga cattgaagct
     1801 gtgctgccag agttcgccga ctataaccag cgcatccgtc atcccggtgg ttttcacctg
     1861 ataaatgcag ctgctgaaag gcgctggatg acgccgtcag gtaaggctaa tttcattacc
     1921 agtaaagggc tgttagaaga tccctcttca gcgtttaaca gtaaactggt catggcgaca
     1981 gtacgcagcc acgatcagta caacacgacg atttatggta tggatgatcg ctatcgaggt
     2041 gtattcggtc aacgagatgt ggtctttatg agtgctaaac aagctaaaat ttgccgtgta
     2101 aaaaacggcg aaagagttaa tcttattgcg ctcacgccag acggtaaacg tagctcacgc
     2161 cgcatggata gattaaaagt ggtcatttac cctatggctg accgctcact ggtgacctat
     2221 tttccagaat cgaatcacat gctaacactt gataaccacg atccattaag tggcattcct
     2281 ggctataaaa gtattccggt tgaattagaa ccatcaaatt aattcgcctc ctcatttctt
     2341 ctgctgtcat ccgcacagca gaagaattcc tcattgacca ttgtcgcgca atttactcac
     2401 atgtgctaaa ttaaactgct cgttataaga tatatacctc cgcctacagc tgtaagaaac
     2461 tccgctcagt actgaagcgc cagtcctatt tcccctattc tccagcctgt tatattaagc
     2521 atactgatta acgattttta acgttatccg ctaaataaac atatttgaaa tgcatgcgac
     2581 cacagtgaaa aacaaaatca cgcaaagaga caactataaa gaaatcatgt ctgtaattgt
     2641 ggttgtctta ttactgacac ttacgttgat agccattttt tcggcaattg atcagctggg
     2701 tatttcagaa atgggtcgca ttgcaaggga tcttacacat ttcattatca atagtttgca
     2761 agactgaaaa taaacagcaa attataaata tgaaatgtta aaa
//
LOCUS       DAZFLD010000346         2791 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.348, whole genome shotgun sequence.
ACCESSION   DAZFLD010000346 DAZFLD010000000
VERSION     DAZFLD010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2791
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.348"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            77..2752
                     /gene="adhE"
                     /locus_tag="ACHKBL_004751"
                     /gene_synonym="adhC"
     CDS             77..2752
                     /gene="adhE"
                     /locus_tag="ACHKBL_004751"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HFO5821489.1"
                     /translation="MAVTNVAELNALVERVKKAQREYASFTQEQVDKIFRAAALAAAD
                     ARIPLAKMAVAESGMGIVEDKVIKNHFASEYIYNAYKDEKTCGVLSEDDTFGTITIAE
                     PIGIICGIVPTTNPTSTAIFKSLISLKTRNAIIFSPHPRAKDATNKAADIVLQAAIAA
                     GAPKDLIGWIDQPSVELSNALMHHPDINLILATGGPGMVKAAYSSGKPAIGVGAGNTP
                     VVIDETADIKRAVASVLMSKTFDNGVICASEQSVVVVDSVYDAVRERFATHGGYLLQG
                     KELKAVQDVILKNGALNAAIVGQPAYKIAELAGFSVPENTKILIGEVTVVDESEPFAH
                     EKLSPTLAMYRAKDFEDAVEKAEKLVAMGGIGHTSCLYTDQDNQPARVAYFGQKMKTA
                     RILINTPASQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPKHLINKKTVAKRAEN
                     MLWHKLPKSIYFRRGSLPIALDEVITDGHKRALIVTDRFLFNNGYADQITSVLKAAGV
                     ETEVFFEVEADPTLSIVRKGAELANSFKPDVIIALGGGSPMDAAKIMWVMYEHPETHF
                     EELALRFMDIRKRIYKFPKMGVKAKMIAVTTTSGTGSEVTPFAVVTDDATGQKYPLAD
                     YALTPDMAIVDANLVMDMPKSLCAFGGLDAVTHAMEAYVSVLASEFSDGQALQALKLL
                     KEYLPASYHEGSKNPVARERVHSAATIAGIAFANAFLGVCHSMAHKLGSQFHIPHGLA
                     NALLICNVIRYNANDNPTKQTAFSQYDRPQARRRYAEIADHLGLSAPGDRTAAKIEKL
                     LAWLETLKAELGIPKSIREAGVQEADFLANVDKLSEDAFDDQCTGANPRYPLISELKQ
                     ILLDTYYGRDYVEGETAAKKEAAPAKAEKKAKKSA"
BASE COUNT          718 a          718 c          687 g          668 t
ORIGIN      
        1 catcaccgca ctgactatac tctcgtattc gagcagatga tttactaaaa aagtttaaca
       61 ttatcaggag agcattatgg ctgttactaa tgtcgctgaa cttaacgcac tcgtagagcg
      121 tgtaaaaaaa gcccagcgtg aatatgccag tttcactcaa gagcaagtag acaaaatctt
      181 ccgcgccgcc gctctggctg ctgcagatgc tcgaatccca ctcgcgaaaa tggccgttgc
      241 cgaatccggc atgggtatcg tcgaagataa agtgatcaaa aaccactttg cttctgaata
      301 tatctataac gcctataaag atgaaaaaac ctgtggtgtt ctgtctgaag acgacacttt
      361 tggtaccatc actatcgctg aaccaatcgg tattatttgc ggtatcgttc cgaccactaa
      421 cccgacttca actgctatct tcaaatcgct gatcagcctg aagacccgta acgccattat
      481 cttctccccg cacccgcgtg caaaagatgc aaccaacaaa gcggctgata tcgttctgca
      541 ggctgctatc gctgccggtg ctccgaaaga tctgatcggc tggatcgatc aaccttctgt
      601 tgaactgtct aacgcactga tgcaccaccc agacatcaac ctgatcctcg cgactggtgg
      661 tccgggcatg gttaaagccg catacagctc cggtaaacca gctattggtg taggcgcggg
      721 taacacgcca gttgttatcg atgaaactgc tgatatcaaa cgtgcagttg catctgtgct
      781 gatgtccaaa accttcgaca acggcgtaat ctgtgcttct gaacagtctg ttgttgttgt
      841 tgactctgtt tatgacgcag tacgtgaacg tttcgcaacc cacggcggct atctgttgca
      901 gggtaaagag ctgaaagctg ttcaggatgt tatcctgaaa aacggtgcgc tgaacgcggc
      961 tatcgttggt cagccagcct ataaaattgc tgaactggca ggcttctctg taccagaaaa
     1021 taccaagatt ctgatcggtg aagtgaccgt tgttgatgaa agcgaaccgt tcgcacatga
     1081 aaaactgtcc ccgactctgg caatgtaccg tgctaaagat ttcgaagacg cggtagaaaa
     1141 agcagagaaa ctggttgcta tgggcggcat cggtcatacc tcttgcctgt acactgacca
     1201 ggataaccaa ccggctcgcg ttgcctactt cggtcagaaa atgaaaacgg cccgtattct
     1261 gatcaacacc ccggcgtctc agggtggtat cggtgacctg tataacttca aactcgcacc
     1321 ttccctgact ctgggttgtg gttcctgggg tggtaactcc atctctgaaa acgttggtcc
     1381 gaaacacctg atcaacaaga aaaccgttgc taagcgagct gaaaacatgt tgtggcacaa
     1441 acttccgaaa tctatctact tccgccgtgg ctctctgcca atcgcgctgg atgaagtgat
     1501 tactgatggc cacaaacgtg cgctcatcgt gactgaccgc ttcctgttca acaatggtta
     1561 tgctgatcag atcacttccg tactgaaagc agcaggcgtt gaaactgaag tcttcttcga
     1621 agtagaagct gacccgaccc tgagcatcgt tcgtaaaggt gcagaactgg caaactcctt
     1681 caaaccagac gtgattatcg cgctgggtgg tggttccccg atggatgccg cgaagatcat
     1741 gtgggtgatg tacgagcatc cagaaactca cttcgaagaa ctggcgctgc gctttatgga
     1801 tatccgtaaa cgtatctaca agttcccgaa aatgggcgtg aaagcgaaaa tgatcgctgt
     1861 caccaccact tctggtacag gttctgaagt cactccgttt gcggttgtaa ctgacgacgc
     1921 tactggtcag aaatatccgc tggcagacta tgcgctgacc ccggatatgg cgattgtcga
     1981 tgccaacctg gttatggaca tgccgaagtc cctgtgtgct ttcggtggcc tggacgcagt
     2041 aactcacgcc atggaagctt atgtttctgt actggcatct gagttctctg atggtcaggc
     2101 actgcaggca ctgaaactgc tgaaagaata tctgccagcg tcctaccacg aaggatctaa
     2161 aaatccggta gcgcgtgaac gtgttcacag tgcagcaact atcgcgggta tcgcgtttgc
     2221 gaacgccttc ctgggtgtat gtcactcaat ggcgcacaaa ctgggttccc agttccatat
     2281 tccgcacggt ctggcaaacg ccctgctgat ttgtaacgtt attcgctaca acgcgaatga
     2341 caacccgacc aagcagactg cattcagcca gtatgaccgt ccgcaggctc gccgtcgtta
     2401 tgctgaaatt gctgaccacc tgggtctgag cgcaccgggc gaccgtactg ctgctaagat
     2461 cgagaaactg ctggcatggc tggaaacgct gaaagctgaa ctgggtattc cgaaatctat
     2521 ccgtgaagct ggcgttcagg aagcagactt cctggcgaac gtggataaac tgtctgaaga
     2581 tgcattcgat gaccagtgca ccggcgctaa cccgcgttac ccgctgatct ccgagctgaa
     2641 acagattctg ctggatactt actacggtcg tgattatgta gaaggcgaaa ctgcagcgaa
     2701 gaaagaagcc gctccggcta aagctgagaa aaaagcgaaa aaatccgctt aatcagtagc
     2761 gctgtctggc aacataaacg gccccttctg g
//
LOCUS       DAZFLD010000347         2785 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.349, whole genome shotgun sequence.
ACCESSION   DAZFLD010000347 DAZFLD010000000
VERSION     DAZFLD010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2785
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.349"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            355..804
                     /gene="glmS"
                     /locus_tag="ACHKBL_004752"
     CDS             355..804
                     /gene="glmS"
                     /locus_tag="ACHKBL_004752"
                     /EC_number="5.4.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050097 - methylaspartate mutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylaspartate mutase subunit S"
                     /protein_id="HFO5821490.1"
                     /translation="MKKATLVIGVIGADCHAVGNKVLDRVFSNHDFRVINLGVMVSQD
                     EYIDAAIETGADAIVVSSIYGHGDIDCLGMRERCIERGLGDILLYVGGNLVVGKHDFA
                     DVETKFKEMGFDRVFAPSHDLEDVCQLMAHDINQRHDVDTRILEEAI"
     gene            804..2192
                     /gene="glmL"
                     /locus_tag="ACHKBL_004753"
     CDS             804..2192
                     /gene="glmL"
                     /locus_tag="ACHKBL_004753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylaspartate mutase accessory protein GlmL"
                     /protein_id="HFO5821491.1"
                     /translation="MQIVSVDIGSTWTKAALFAREGDALTLVNHVLTPTTTHHLAKGF
                     FSSLDQVLNVDDALPLLNSGEVALKYSSSAKGGLAVAAMGLVPSITLETAKVTAHSAG
                     AKIAQSYAYKLNRRDIQALEETQPDILLFTGGTDGGEESYGLNNARVLAESKLDCAII
                     YAGNRDIQDEVQEILGHKDLTIVDNVLPDLDHPNPLAARQAICDIFLKRIVKGKGLDV
                     VVDKTGEEPMPTPWTVFELVKAISNVDHSWKEFMLIDMGGATTDVYSASANTLSPDTV
                     LHGVPEPFVKRTVEGDLGMRVSAMVVGESAKELVNVNFAQQPAQLDAFYHYLRHLVAH
                     PDYLPANAEEKYFDTVLAGLCVGYATERHAGTKKEVCTCVGNVDLQMGRDLTTVRKVV
                     GSGGWLSRASQFDMHHWLKYRELNDDGKRILLPTEFEYYRDSRGLLPLLANVARVDPL
                     AAARTSIQCLTL"
     gene            2211..2696
                     /locus_tag="ACHKBL_004754"
                     /pseudo
     CDS             2211..2696
                     /locus_tag="ACHKBL_004754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308789.1"
                     /note="methylaspartate mutase subunit E; catalyzes the
                     formation of threo-beta-methyl-L-aspartate from
                     L-glutamate; incomplete; partial in the middle of a
                     contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methylaspartate mutase subunit E"
BASE COUNT          782 a          676 c          671 g          656 t
ORIGIN      
        1 atgaattgca gtgaaattcg acttaacttt tatgaaatgc atttactcat ggctaacccc
       61 atatcactaa aatgcaaatt agaagttgat cacaattatc tcaatacatc aaatcaagtt
      121 aattttaaaa atttaacatt catgacatta gttgacataa cacactgaat taacatgaga
      181 atttgaaaac acactttaaa caatcatttc ccgaaagaca ttttgaaaat agatcaattg
      241 caacattacg gtcagataaa gacactcctg tgatattaaa cctgtgccgt tatgagcggc
      301 cataaatacg acgtgtgtaa cttattttac tctctgtctt acaaaggatt acccatgaag
      361 aaagcaacac ttgtgattgg cgttattggc gcggactgcc atgcagtagg caataaagtt
      421 ctggaccgcg tttttagcaa ccatgacttc agagttatta atctgggcgt aatggtcagc
      481 caggacgaat atattgatgc tgccattgaa actggtgctg acgcgattgt cgtctcctct
      541 atctatgggc atggcgatat cgactgtctg ggcatgcgcg aacgctgcat cgagcgtgga
      601 ctgggggaca tcctgctcta tgtgggcggt aacctggtgg tcggaaaaca tgacttcgct
      661 gacgtggaaa ccaaattcaa agagatggga tttgatcgcg tctttgcccc aagtcatgac
      721 ttagaagatg tttgtcagct gatggcccac gatatcaacc aacgtcatga cgttgatact
      781 cgtattcttg aagaggcaat ctgatgcaaa tcgtctctgt cgatattgga tcgacgtgga
      841 ccaaagcagc cctcttcgcc cgggaggggg acgcgttaac gttggttaac cacgtactga
      901 ccccaaccac cacccatcat ctggcgaagg ggtttttctc cagcctggac caggtgttaa
      961 acgtagatga tgccctcccc ctgttaaaca gtggtgaggt tgcattgaag tattcctcct
     1021 ctgccaaagg tgggctggcg gttgccgcaa tgggattggt gccttctatc accctggaaa
     1081 ccgcgaaagt aaccgctcac tctgcgggtg ctaaaattgc gcaatcttac gcctacaagc
     1141 tgaaccgccg tgatatccag gcactggaag aaacgcagcc cgatattctc ttgttcaccg
     1201 gcggcaccga tggcggagaa gaaagctacg gactgaataa cgcccgtgtg ttggcagaat
     1261 ccaaactcga ctgcgccatt atttatgccg gaaaccggga tattcaggac gaggtgcagg
     1321 agatcctcgg gcataaagac ctgaccatcg tcgacaacgt tctgcccgat cttgaccatc
     1381 cgaacccttt agctgcacgc caggctatct gcgacatatt ccttaaacgc atcgttaaag
     1441 ggaaagggct ggatgtcgtt gttgataaaa caggcgaaga accaatgccg acaccgtgga
     1501 cagtctttga actggttaag gcaatcagta atgtcgatca cagctggaaa gagttcatgc
     1561 tgatcgacat gggtggcgca accaccgatg tctattccgc cagtgccaac acgctctcgc
     1621 ccgacactgt tttacatggc gtaccggaac ccttcgtaaa acggacggtg gaaggagatc
     1681 tgggaatgcg cgtctcggca atggtggttg gtgaaagtgc gaaagagtta gtcaatgtga
     1741 attttgctca gcaacccgca cagctggacg ctttttatca ttacctccgt catctagtcg
     1801 ctcatcccga ctacttaccc gcgaatgcag aagaaaaata tttcgatacc gtcctggccg
     1861 gactgtgtgt gggttatgcc accgaaagac atgcgggcac caaaaaagag gtctgcacct
     1921 gcgtcggcaa tgtcgattta cagatgggac gtgacctgac aaccgtgcgt aaagtcgtcg
     1981 gatccggcgg atggctctcg cgtgccagtc agttcgatat gcatcactgg ttgaaatacc
     2041 gcgagttaaa cgacgacggt aaacgcattc tcttaccgac cgaatttgaa tattaccgcg
     2101 attcccgggg gctattacca ctgctggcaa acgtcgccag agtcgatccg ctggccgccg
     2161 cgcgcaccag tattcaatgc ttaaccctat aacaattaag gctgcaatca atggaacttc
     2221 gaaataagaa attaacccat gacgaattca tgaccgagcg gcaacaggta ctgaaaacct
     2281 gggaaaccgg caaggatgtt gaaaactttg aagatggcgt taaataccaa caaaccattc
     2341 ctgagcataa acgcttctct cttgcgctgt tgaaagcaga caaggaaggt aaaaccctta
     2401 gccaaccgcg cgcaggtgtg gcgctgatgg acgaacacat cgaactactg aaaacactac
     2461 aagaagagtg cgatctgctg ccgagcacca tcgatgcata tacccgtctg aatcggtacg
     2521 aagaagcggc tgtagggatt aaaaaatcca tcgaagccgg aacctctaaa ctgaacggtt
     2581 taccggtcgt taaccacggc gttgcagctt gccgtcgcct caccgaaact ctgcaaaaac
     2641 cgctacagat ccgccacggc acgccagatg cgcgtctgct ggctgaaatc tcgatgtgta
     2701 tacatgaata tactctaaat aagtcaagtt gcaggaaggg aacaaataat gaatttccag
     2761 aagcttacat aagtaagtga cctgg
//
LOCUS       DAZFLD010000348         2776 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.350, whole genome shotgun sequence.
ACCESSION   DAZFLD010000348 DAZFLD010000000
VERSION     DAZFLD010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2776
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.350"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>2776
                     /locus_tag="ACHKBL_004755"
     CDS             <1..>2776
                     /locus_tag="ACHKBL_004755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5821492.1"
                     /translation="PDGRTVREKLRHEIIHHAMEHVVTPAEYQTIIKTVLKTRDSDNA
                     TIREAWRKVDASYRKESPEVQAGEFLAHMAEKQPNKFVAAWERVVALVKGVLRRTGLL
                     KPTELNDIRLVRETIRTLGQRVREGYTPREDGAGASSQYSRSGKPDPFKVPEGEGERY
                     RDDLARMMKSLRTTDLTVNIGRTPPVLRHLGAPDLPLVISRDTVRKATNGVKHVVPMD
                     VIERLPELMHDPDAIYRSATERNAVVMLLDAVDKNGDPVVSAVHMKAVRSRLEINKVA
                     SVYGTEGGMSKANSLDKAGLTLYRKKKLSRDNLLHRGLQLPKGEHSYRGSVDKILYPE
                     DIRKGPYYSRTSSLTPEETIASRFVRQMQDKFQVLKAVQENIRKTGGKVDDSNNAYMA
                     EELFHGKAENDLNVMKERYVQPLAKLLADYKIAQADLDEYLYARHAPERNAHIAKINP
                     KMPDGGSGMTNAEAAEIMQRVRNSGKQAQYDRLAGIVDDMLARRRELIREAGLEESGV
                     VDAWQNAYRYYVPLKGQDVDGVVSLPRTGKGFTIGGRESRQAMGRASRAQSPSTQAIQ
                     DLSESLIRNRKNEVGNAFLKLVQDNPDKDYWQVFTDDKPDTMRAIAERVDPETGETRR
                     EVVERPVPMAMMADRYFTTKKDGKTYYIKLHDPRLMRAMKNMGPETSNAVIRTLGKVN
                     RFLATVNTSYNPEFLVSNFIRDVQTAVMNLKAEQGRSDGKLKGLDNLSALAVVKDSRS
                     AMSAVYASLRGKTLTGKGTQWQKVWKEFVEDGGKTGWFNMGDLEGQQKEMDRLVSLAK
                     GGWKGQSIGAWNSFLNLVEDANGAVENALRLSAYKHARDAGLSRQQAASLAKNMTVNF
                     NRRGEQGALMNSLYMFANASIQGTANLVRTLGHLNGEGPLLERLRWKNLNVPQKIALA
                     AVGAGYLLGSL"
BASE COUNT          703 a          666 c          838 g          569 t
ORIGIN      
        1 cctgacgggc ggaccgtacg cgagaagttg cgtcacgaga ttatccacca tgccatggag
       61 catgttgtca cgccagcgga atatcagacg attatcaaaa ccgtgctgaa aacccgcgac
      121 agtgataacg ccaccatccg tgaagcatgg cgtaaggttg atgcgtccta tcgtaaggaa
      181 tcaccggaag tgcaggcggg tgaatttctg gcgcatatgg cagagaaaca gccgaataaa
      241 ttcgtggcgg catgggagcg tgttgttgcc ctggtcaaag gggtactgcg tcgtacgggg
      301 ttactgaaac cgacggaact gaacgatatc agacttgttc gcgagaccat ccgtacgtta
      361 ggccagcgtg tgcgggaagg ttacacgccg cgtgaggatg gcgcgggcgc atcgtctcag
      421 tactcccgta gtggtaaacc tgatccgttc aaagtgccgg aaggtgaggg cgagcgttat
      481 cgtgatgacc ttgccagaat gatgaaatct ctgcgcacca cagatttaac ggtaaacatc
      541 gggcgtacgc cgccggtatt gcgtcacctt ggtgcaccgg atttgccgct ggttatttcc
      601 cgcgatactg tgcggaaggc caccaatggt gtgaaacatg tggtgccgat ggatgttatc
      661 gagagactcc cggaactgat gcacgatccg gatgcaattt accgctcagc gacagaaaga
      721 aatgcggttg tgatgctgct tgatgccgtg gataaaaatg gtgatccggt ggtgtcagcg
      781 gtacacatga aggctgtccg gtcgcgtctg gaaatcaaca aggtagcttc tgtttacggt
      841 acggaaggag gaatgagtaa ggcaaatagt ctggataaag ccggattaac gctttaccgg
      901 aagaaaaaat taagccgcga taaccttctg cacagagggc ttcaattgcc caaaggggaa
      961 cattcttatc gcggctctgt ggataaaata ctctatcctg aagatattcg caaggggccg
     1021 tattactccc gtaccagcag tctgacgccg gaagagacta ttgcatcgcg ttttgtgcgc
     1081 cagatgcagg ataaattcca ggtgctgaaa gctgttcagg agaatatccg taaaacgggc
     1141 ggcaaagtgg acgacagtaa caacgcttat atggcggaag aactcttcca cgggaaggcg
     1201 gaaaacgacc tgaacgtgat gaaggagcgc tatgttcagc cactggctaa attactggcg
     1261 gactacaaaa ttgcgcaggc cgatctggat gagtacctct acgcccgtca cgcgccggaa
     1321 cgtaacgcgc atatcgcgaa aatcaacccg aaaatgccgg acggcggttc ggggatgacc
     1381 aacgcggaag cggcggaaat catgcagcgt gtgcgtaaca gtggcaaaca ggcacagtat
     1441 gaccgtctgg cagggattgt tgacgatatg ctggcccgtc gccgtgagct tatccgtgag
     1501 gccggactgg aagagagcgg tgtggtggat gcctggcaga acgcctaccg ttactacgtt
     1561 cccctgaaag gtcaggatgt tgacggtgtg gtgtcactgc cccgtacagg taagggcttc
     1621 accatcggcg gacgtgaaag caggcaggcc atggggcgtg catcccgcgc acagtctcct
     1681 tccactcagg cgatacagga tttgagcgaa tcgctgatcc gcaatcgcaa aaacgaagtg
     1741 ggtaacgcct tcctgaaact ggtgcaggat aatcccgata aggattactg gcaggtattc
     1801 actgatgaca aaccggacac catgcgggcg attgcagagc gtgttgatcc ggaaaccggc
     1861 gaaacccgtc gcgaagttgt tgaacgtccg gtgcctatgg caatgatggc ggatcgctac
     1921 ttcaccacca aaaaggacgg caaaacttac tacatcaaac tccatgaccc gcgcctgatg
     1981 cgtgcgatga agaacatggg gccggaaacc agtaacgccg taatccgtac gctgggtaaa
     2041 gttaaccgct tcctggcaac ggtgaacacg tcgtataacc cggaattcct ggtcagtaac
     2101 ttcatccgtg acgtgcagac ggcggtgatg aacctgaagg cggagcaggg aaggagcgac
     2161 ggtaaactga aagggctgga taacttatcc gcactggctg tggtgaaaga cagtcggtct
     2221 gccatgtctg ccgtatacgc cagtctgcga ggcaaaactc tcacgggcaa aggtacacag
     2281 tggcagaagg tgtggaaaga gtttgttgag gacggaggca aaaccggctg gtttaacatg
     2341 ggtgaccttg aaggccagca gaaggaaatg gatcgccttg tctcactggc gaagggggga
     2401 tggaaaggcc agagtatcgg tgcatggaat tcgttcctga acctggtcga ggatgccaac
     2461 ggcgcggttg aaaacgctct gcgtctttct gcctataaac atgcccgtga tgccggtttg
     2521 tcacgccagc aggcggcgtc tcttgccaaa aacatgacgg tgaactttaa ccgtcgtggt
     2581 gagcaggggg cgctgatgaa ctcgctgtac atgttcgcca acgccagtat tcagggaacg
     2641 gcaaacctgg tgagaacgct cggacatctt aatggtgagg gacctttact ggagcgcctt
     2701 cgctggaaga atctcaatgt gccgcagaaa atcgcgcttg ccgctgttgg agcaggttat
     2761 ctgcttggct cgctta
//
LOCUS       DAZFLD010000349         2764 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.351, whole genome shotgun sequence.
ACCESSION   DAZFLD010000349 DAZFLD010000000
VERSION     DAZFLD010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2764)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2764)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2764
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.351"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(1..2046)
                     /gene="mchF"
                     /locus_tag="ACHKBL_004756"
                     /pseudo
     CDS             complement(1..2046)
                     /gene="mchF"
                     /locus_tag="ACHKBL_004756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001529577.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="microcin export peptidase/ABC transporter MchF"
     gene            complement(2039..>2764)
                     /locus_tag="ACHKBL_004757"
     CDS             complement(2039..>2764)
                     /locus_tag="ACHKBL_004757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000489618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFO5821493.1"
                     /translation="INKDQLTNQVALYYQQQNNLLSLSGQNEQNALQITTLESQIQTQ
                     AADFDNRIYQMELQRYELQKELVNTDVEGEIIIRALTDGKVDSLSVTVGQMVNTGDSL
                     LQVIPENIENYYLILWVPNDAVPYISAGDKVNIRYEAFPAEKFGQFSATVKTISRTPA
                     STQEMLTYKGAPQNTPGDSVPWYKVIAMPEKQIIRYDEKYLPLENGMKAESTLFLEKR
                     RIYQWMLSPFYDMKHSATGPLND"
BASE COUNT          761 a          709 c          550 g          744 t
ORIGIN      
        1 caataatcac ccgggtgata ttcatctttt ttatagcagc attcacgaaa cgttcacttt
       61 ctgtatcaag agaacttgta gcctcatcca taaataatat tccaggtttc cggtataaag
      121 ctcgggcaat gaatatacgc tgtttttgac cgccggaaag accttccccc agttcaccta
      181 ttaacgtttc ataccccatt ggcattttca ttatcacatc atgaatatga cttgctttgg
      241 cacattctgt catccattcg tcgtctgttt cttctgcaaa cccacagata ttttcacgaa
      301 ttgatcctga aaatagtcgg tcgtcctgca taacacaggc tatcatacgg tgataattat
      361 ttattccaag ttgacgtata tccgtgccat taaccagtac ttttccacta tctggttcaa
      421 acagtccaca taatactttc attaatgtgg tttttccggc accggaggca ccagttatag
      481 ccacactttc tcccggagcc acagacaaat tcagaccact gaatacctgt gctgactggc
      541 tgtcataccg gtagcttaaa tcagtggttt ccagtgaaac cgggctcatg tcagcaacga
      601 tttcaatttc cggtttcttt tcttcctttt catgtagtgc aatatctgca atgagctcat
      661 tatgcagact cattattctc agttgaagaa gaaaactggt cagcgaagca acccgatccg
      721 aaaactgccc acgaaaagaa ccaaatgcca caaacattcc tattgtcatc tgattatcga
      781 tcacaaggct tgcacccagc cataaaatcg ccacctgatc acaggcggca acaaaagtat
      841 ttatcccccc gaagagcaaa tccatcctgg ttaacttaat acctgaattg atcgcatcta
      901 ttttcaggtt aagccagtgt gttccccgaa tcccagccat accctggatt tttaccgtgg
      961 caataccata caggctttcc ataaaatagg agctggcccg ggccccctga caagagtttc
     1021 ttccgatatt tgccggtaat agccgtatgt caccagacga ataagaacgt aaaccatggt
     1081 aaaaccgagc actatccagg taagatatcc tccatataac agcatcatca caaaaacacc
     1141 cacaaccata atactgtcca tgattgcccc aacaacacag gtggtaaagg tggccctcaa
     1201 agtgtcaagg gagccaaaac gcgactggat atcacctaat ttacggcgtt caaaaaacgc
     1261 cagcggcagt ctgagaagat ggttaaacag acccgactgc cactgtatat tgatgagcgt
     1321 gctcataacc agtgaggacc atgcacgcag catactcacc gcggccctga gcaatataaa
     1381 gaacatcagg ccagcagaaa taagcgtcag cagccctctg tcccccgccg gaatcgcatg
     1441 atccataacc agctgagttc ccaccggcat taccagattg attgcttcaa ttacaactga
     1501 cagacagaaa attttcgcca gtgttctttt aataccgtaa atactgttaa tcagtgaacg
     1561 gagacttatg cgggtctgct gggtttccgc caggaattca cttccgggcc agacctcaag
     1621 tgcaatgccc gtaaaatacc ggctcatttc ctcccgaccg agatatcttc tgcctctggc
     1681 cggatcatgc agtacataac ggttacgctt tacgctgacc agcacgacga agtgactgaa
     1741 atcccagtgg agaatacacg gcattttgag ggcaccaagt tcatccagct ccagtgaaag
     1801 agcccgggtg accatcccca gctgctccgc tattccattg attcctgcaa ggtttgctcc
     1861 acgggccgag agattaaact tccggcgaag agatatcagg tcaatattct taccaaaatg
     1921 accgcatatc attgccaggc aggccagtcc acattcagcg gtctccgtct gatgaataac
     1981 cggaacacga cgtcgccagc gcatatcaag ctgatttata atttgtctga aattcccgtt
     2041 agtcattgag cggtcctgtt gcactgtgtt tcatgtcata gaaaggagaa agcatccact
     2101 ggtaaatacg ccttttttcc agaaatagtg tactttcggc tttcattcca ttttccagag
     2161 ggaggtattt ttcgtcatat ctgataatct gcttttcagg catcgcaatg actttatacc
     2221 agggaacaga gtcgcccggc gtattctgtg gtgctccctt ataggtcaac atttcctgtg
     2281 ttgacgcagg agtcctggat atagttttaa ccgtagcaga gaactgccca aatttttctg
     2341 ccggaaaggc ttcataacga atattcactt tgtcaccagc cgaaatataa ggaacagcat
     2401 cattcgggac ccagagaata agataatagt tttcaatgtt ctcaggaata acctgcagaa
     2461 ggctgtctcc ggtattgacc atttgcccga cagtgacact cagggagtca actttcccgt
     2521 cagtcaacgc ccggataatg atttcgccct ccacatcagt gttaaccagt tctttctgta
     2581 actcgtaccg ttgcagttcc atctggtaga tacggttatc aaaatctgca gcctgagtct
     2641 gaatctgact ctccagagtg gttatctgca gagcattctg ttcgttctgt ccgctcaggc
     2701 tgagaagatt gttttgttgc tgataatata atgccacctg gttagttaac tgatctttat
     2761 taat
//
LOCUS       DAZFLD010000350         2762 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.352, whole genome shotgun sequence.
ACCESSION   DAZFLD010000350 DAZFLD010000000
VERSION     DAZFLD010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2762
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.352"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(25..222)
                     /locus_tag="ACHKBL_004758"
     CDS             complement(25..222)
                     /locus_tag="ACHKBL_004758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HFO5821494.1"
                     /translation="MKSLTTETALDILIAWLQDNIDCESGIIFDNDEDKTDSAALLPC
                     IEQAREDIRTLRQLQLLHQNR"
     gene            complement(234..722)
                     /locus_tag="ACHKBL_004759"
     CDS             complement(234..722)
                     /locus_tag="ACHKBL_004759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
                     /protein_id="HFO5821495.1"
                     /translation="MKLALTLEADSVNVQALNMGRIVVDVDGVNLTELINNVAENGYS
                     LRVVEESDQQSTCTPPPFATLAGIRCSTAHITETDNAWLYSLSHQTSDVGESEWIHFT
                     GSGYLLRTDAWSYPVLRLKRLGLSKTFRRLVVTLTRRYGVSLIHLDASAGCLPGLPTF
                     DW"
     gene            complement(719..1096)
                     /gene="cbtA"
                     /locus_tag="ACHKBL_004760"
     CDS             complement(719..1096)
                     /gene="cbtA"
                     /locus_tag="ACHKBL_004760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000854828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system
                     cytoskeleton-binding toxin CbtA"
                     /protein_id="HFO5821496.1"
                     /translation="MKTLPVLPGQAASSRPSPVEIWQTLLTRLLDQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPEFSACTRSQLINSIDILRARRVTG
                     LMTRDNYRTVNNITLGKYQEAKR"
     gene            complement(1185..1553)
                     /gene="cbeA"
                     /locus_tag="ACHKBL_004761"
     CDS             complement(1185..1553)
                     /gene="cbeA"
                     /locus_tag="ACHKBL_004761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system cytoskeleton
                     bundling-enhancing antitoxin CbeA"
                     /protein_id="HFO5821497.1"
                     /translation="MSDTLPGTTHPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYL
                     ADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSGELNPRHQHTVTLYAKGLTCEAD
                     TLGSCGYVYLAVYPTPEMKN"
     gene            complement(1629..1850)
                     /locus_tag="ACHKBL_004762"
     CDS             complement(1629..1850)
                     /locus_tag="ACHKBL_004762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HFO5821498.1"
                     /translation="MKIVTRGEAMRIHQQHPTSRLFPFCTGKYRWHGSAGAYTGREVQ
                     DIPGVLAVFAERHKDSFGPYVRLMSVTLN"
     gene            complement(1919..2395)
                     /gene="radC"
                     /locus_tag="ACHKBL_004763"
     CDS             complement(1919..2395)
                     /gene="radC"
                     /locus_tag="ACHKBL_004763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFO5821499.1"
                     /translation="MQQLSFLPGAMTPGELSLILRALKTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALALVDIRVPDHLIVGGSQVFSFAEHGLL"
     gene            complement(2410..>2762)
                     /locus_tag="ACHKBL_004764"
     CDS             complement(2410..>2762)
                     /locus_tag="ACHKBL_004764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFO5821500.1"
                     /translation="LIPQWVTLEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEP
                     DDDDDETWVLFNAMNGNRAEMSPEAAGIAACLMTYSHHACRTECYAMTVHYYRLRDYA
                     LQHPECSAIMRIID"
BASE COUNT          634 a          709 c          793 g          626 t
ORIGIN      
        1 ggaatgaagt ctggtgagtg agattcaccg gttctggtgc agaagctgca gctggcgcag
       61 ggtacggata tcctccctgg cctgttcaat acagggcaac agtgctgccg aatccgtttt
      121 atcttcatcg ttgtcaaaga taattcccga ttcgcagtcg atattgtcct gcagccatgc
      181 aatcagaata tccagcgctg tttccgtggt taatgatttc atgttgtttc tggttaccag
      241 tcgaaagtgg gtaaacccgg cagacatccg gcactggcat ccagatgaat gagactgacg
      301 ccataacgtc gggtaagtgt gacaaccaga cggcggaacg tttttgacag gcccaggcgc
      361 ttaagccgca gaaccgggta tgaccacgca tcggtacgta acagatagcc gctacctgtg
      421 aaatgaatcc attctgattc accaacgtca ctggtctggt gtgacagcga gtacaaccag
      481 gcgttgtccg tttccgtgat atgtgcggta ctgcagcgta tgccggcaag ggttgcaaat
      541 ggcggaggtg tgcaggttga ctgttggtcg gattcctcca ccacacggag tgaataaccg
      601 ttttcagcga cgttattaat cagttcagtg agattaacac catcgacgtc aacgacaatg
      661 cgccccatgt tcagtgcctg cacgttaacg ctgtcagctt ccagcgtcag ggccagtttc
      721 atcgtttcgc ctcctgatac ttacccaggg taatgttatt taccgttcgg taattgtcgc
      781 gggtcatcag gccagtcacc cggcgagccc ggaggatatc gatgctgttt attaactgag
      841 agcgggtaca ggcactgaat tccggctggt cggtacgcac cagcgcgtat ttttcaacaa
      901 gaaagttcac tgcatcgcac agtgaaatgc cggcctcaat atgctgctca atcacacgtt
      961 catcagcgaa cggtgtgtca ttcagcgtaa ggccatagtg ctggtccagc agtcgggtga
     1021 gcagtgtctg ccagatttca acaggagacg ggcgagaact ggccgcctgc ccgggtaata
     1081 caggtaatgt tttcatactg aagattttcc tgatatgcag atataaaaat ggaaaagtgg
     1141 cgtggtgaaa acaccgggcc gtagcagaag gctattctgg agagttaatt tttcatttcg
     1201 ggcgtcggat aaacagccag ataaacgtaa ccacaggagc cgagggtgtc ggcttcacag
     1261 gtcagcccct tcgcatacag tgtcacggta tgctgatggc ggggattcag ttcaccgctg
     1321 gtgagcatga gctccagttg tttcatcagc agcggaaagg cctggtccag atggtacgca
     1381 tccgcgtcgc tgaaccggcc tctgataccg gcgcggtcag caaggtaatg caaccggtta
     1441 ccctcctgta ccagtcgggc cccgaaacag ggcgtcacgg tgcagggcag cccccaccag
     1501 gggcggtcgt gattgtcgtc gggatgcgtt gtcccgggga gtgtgtctga cacgataaag
     1561 tcctcacaaa aaatcggtta agaatggtcc ggtggtggcg ataattctgc tcatcagaat
     1621 gccctgattc agttcagggt gacgctcatc agccggacat acgggccaaa actgtcctta
     1681 tggcgttcag caaacacggc cagcacaccg ggaatatcct gcacttcacg accggtatac
     1741 gccccggcac tgccgtgcca gcggtacttg ccggtgcaga acggaaaaag acgggatgtc
     1801 ggatgttgtt ggtgaatacg catggcttca ccacgggtga cgatttttat agtgtgatac
     1861 ctctgaagac agaagataaa agcgaaaaca ggtatgatgt ggttgtgacg gtgacaggtt
     1921 aaagcagacc gtgttccgca aaggagaaaa cctggctgcc accgactatc agatggtccg
     1981 gcacccggat gtcaaccagg gccagcgcct gtaccagacg ttcggtgata agccggtctg
     2041 ccttactggg tgtgacttca ccggacgggt gattgtgtgc cagcaccacg gcagcggcat
     2101 tgtggtacag ggcgcgttta atcacttccc ggggatggac ttccgtgcgg ttgatggtgc
     2161 cggtaaagag ggtttcaccg gcaatcagct gattctggtt gttcagatac agcacccgga
     2221 attcttcacg ctccagtccc gccatgttca gaatcagcca ttcccgtgcc gctcgggtgg
     2281 aggtgaaggc cacgccgggt tcatgaagat ggcggtccag ggttttcagg gcccgcagaa
     2341 tgaggctgag ctcgccgggc gtcatcgcgc cgggcagaaa ggaaagttgt tgcattgtac
     2401 ttctcctgct cagtcgatga tgcgcataat ggcgctgcat tccggatgct gcagggcgta
     2461 atcccgcagc cggtaataat ggaccgtcat ggcataacac tccgtacgac aggcgtgatg
     2521 actgtacgtc atcagacagg cggcaatgcc ggcggcttcc gggctcattt cagcgcggtt
     2581 accgttcatg gcattgaaca gtacccatgt ttcgtcatca tcgtcatccg gttcgggtgc
     2641 cataaatgcc ccgccgttgt tcagggtata cagattccag ataccaccac agtagtcttc
     2701 gcaaagacgg tccatccagc cgaaaacacg gggctccagg gtcacccact gtggaatgag
     2761 gc
//
LOCUS       DAZFLD010000351         2747 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.353, whole genome shotgun sequence.
ACCESSION   DAZFLD010000351 DAZFLD010000000
VERSION     DAZFLD010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2747
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.353"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..2390)
                     /locus_tag="ACHKBL_004765"
     CDS             complement(<1..2390)
                     /locus_tag="ACHKBL_004765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001556811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system Vgr family protein"
                     /protein_id="HFO5821501.1"
                     /translation="MSIPALNFDHSHHKLKIRGLQSPVDVLTFEGREQLSTPFRYDIQ
                     FTSTDKAITPELVLMQDGAFSLTAPPVQGMPVQAPLRTLYGVITGFKHLSSSQDEARY
                     EVRLEPRMALLARSRQNAIYQNLTVPQIVEKILRERHQMRGQDFVFNLKSEYPAREQV
                     MQYGEDDLTFISRLLSEVGIWFRFATDARLKIEVVEFYDDQSGYERGLTLPLRHPSGL
                     FDGETEAVWGLNTAYSVVEKSVTTRDYNYRTATAEMMTEQHDATGGDNTTYGEAYHYA
                     DNFLQKGDKEAAESGAFYARIRHERYLNEQAILKGQSTSSLLMPGLEIRVQGDDAPAV
                     FRKGVLITGVTASAARDRSYELTFTAIPYSERYGYRPAFIPRPVMAGTLPARVTSTVK
                     NDIYAHIDKDGRYRVNLDFDRDTWKPGYESLWVRQSRPYAGDTYGLHLPLLAGTEVSI
                     AFEEGNPDRPYIAGVKHDSAHTDHVTIQNYKRNVLRTPANNKIRLDDERGKEHIKVST
                     EYGGKSQLNLGHLVDAGKQQRGEGFELRTDMWGAVRAKKGIFISADAQDKAQGQVREM
                     APAMAILDGAQSQMKSLSTDAQTANADPADLSSQIALLQQSVKDLTQAVILLSAPKGV
                     AIASGEHLQLAAAKNVIANAGNNADVGVVKNMFIGVGQALSVFVRKAGIKLFANKGAI
                     SVQAQNDLMELLAKKEIIITSTEDEIKITAKKKITLNGGGSYIRLDACGIEAGTPGEY
                     NVKAGYYGRKPKAKLTPELMAFPVIEGGDYSRCFKFFDDENIPYRNVKYIANFIDGT"
     gene            complement(2438..>2747)
                     /locus_tag="ACHKBL_004766"
     CDS             complement(2438..>2747)
                     /locus_tag="ACHKBL_004766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5821502.1"
                     /translation="QKTYSLIYTFTKSFKGNLIDLVTNSSFFVETIKSAGIKEPVPWD
                     VFPELEPDTFGSLQGELEFWWESIWSPFWHSLSPDERNDFLVKNNATPQWREFIEFHC
                     "
BASE COUNT          551 a          784 c          709 g          703 t
ORIGIN      
        1 gttccatcaa taaaattggc tatatactta acattacgat atgggatatt ttcgtcatca
       61 aaaaatttaa aacagcgaga atagtctcca ccctcaatca ccggaaacgc catcagctcc
      121 ggcgtcagct tcgccttcgg cttacgccca taatatcccg ccttaacgtt atattctccc
      181 ggcgttcccg cttctatccc acaggcatcc agccggatgt agctgccacc gccgttaagg
      241 gtgatctttt tcttcgccgt gattttaatt tcatcttcgg tactggtaat gatgatttcc
      301 ttcttcgcca acagttccat caagtcgttc tgcgcctgca ccgagattgc gcccttattg
      361 gcaaacagct tgatgcccgc tttacgcaca aacacgctca gcgcctgtcc cacgccgata
      421 aacatatttt tcaccacgcc gacatcggcg ttgttgcctg cgttggctat cacattcttt
      481 gccgccgcca gttgcaggtg ctcgccgctg gcgatcgcca cgcctttggg tgcgcttagc
      541 aggatcaccg cctgcgtcag atcttttaca ctctgctgca acagcgctat ctgtgatgac
      601 agatctgccg gatcggcatt cgccgtctgc gcatcagtgg acagcgactt catctgtgac
      661 tgtgcgccat ccagtattgc cattgccgga gccatttccc gcacctgacc ctgagccttg
      721 tcctgcgcat cggcgctgat aaagatgcct ttcttcgccc gcactgcgcc ccacatatcg
      781 gtacgcagtt caaagccttc gccgcgctgc tgtttcccgg catccaccag atggccgagg
      841 tttagctggc tcttgccgcc gtactcggta ctgaccttga tatgctcttt gccgcgctca
      901 tcgtcgaggc ggattttgtt gtttgctggg gtgcgcagca cgttacgctt gtagttctgg
      961 atggtcacat ggtcggtgtg cgccgagtcg tgctttaccc cggcgatata cggtcggtcc
     1021 gggttgcctt cctcaaacgc gatgctgacc tccgttcccg ccagcagcgg cagatgcagg
     1081 ccgtaggtgt ctcccgcata cgggcgcgac tggcgtaccc acaggctctc ataacccggt
     1141 ttccacgtat cgcggtcaaa atcgaggttg acccggtagc gcccgtcttt gtcgatatgg
     1201 gcatagatat cgttcttcac cgtgctggtg acccgcgccg ggagcgttcc ggccatgacc
     1261 gggcggggga taaacgccgg gcggtagccg tagcgttctg agtacgggat agcggtaaac
     1321 gtcagttcat agctgcggtc acgcgccgcg ctggccgtca cgccggtaat cagtacgccc
     1381 ttgcggaaca ccgctggcgc gtcgtcgccc tgaaccctga tttccagccc cggcatcagc
     1441 agtgaactgg tgctctggcc tttgagaatg gcctgttcat tcaggtagcg ttcgtggcgg
     1501 atacgggcat aaaacgcgcc gctttccgct gcctctttat cgcctttttg caggaagttg
     1561 tcggcgtagt gataggcttc gccgtaggtg gtgttatcgc ccccggtggc gtcatgctgc
     1621 tctgtcatca tctccgctgt ggcggtgcgg tagttgtagt cccgcgtggt gacgcttttt
     1681 tccaccacgc tgtacgcggt gttcagcccc catacggctt ccgtttcacc atcaaacagc
     1741 cctgaaggat gacgaagcgg cagcgtcagc ccgcgctcat agccgctctg gtcgtcataa
     1801 aactcgacaa cctcaatttt cagccgggcg tcggtggcga agcggaacca gatgcccact
     1861 tccgacagca ggcgtgaaat aaacgtcagg tcgtcctcgc catactgcat cacctgttcg
     1921 cgggccgggt attcgctttt gaggttaaac acgaagtcct gaccgcgcat ctggtgccgc
     1981 tcgcgcagga ttttttcgac gatttgcggc accgtcagat tctggtagat ggcattctgg
     2041 cggctgcggg ccagcagcgc catacgcggc tccagccgta cttcgtaacg ggcttcgtcc
     2101 tgtgacgatg acagatgttt aaagccggta atgacgccat acagggtgcg caggggggcc
     2161 tgcaccggca ttccctgtac cggcggtgcc gtcaggctga atgcgccgtc ctgcatcagc
     2221 accaactccg gcgtgatggc tttatcggta ctggtaaact gaatgtcgta gcggaacggg
     2281 gtactcagtt gttcgcggcc ttcgaaggtc agcacatcca ccggactttg caggccgcgg
     2341 attttgagtt tgtggtgtga atggtcaaag tttagggcag gaatactcat cattatttcc
     2401 ctgtatagat agaaatattt tcgtcattaa aaataaatca acaatgaaac tcaatgaact
     2461 ctcgccattg tggtgtcgca ttatttttca ctaagaaatc gtttctttca tcaggtgata
     2521 gactgtgcca gaacggagac cagatactct cccaccagaa ctcaagttct ccttgtaagc
     2581 taccgaaagt atcgggttcc aactcaggaa aaacatccca tggtacaggt tcttttattc
     2641 cagcagattt aatagtttca acaaaaaaag aagagttcgt cactaaatca atcaaattcc
     2701 ctttgaatga tttagtgaat gtgtaaatta aagagtatgt tttttgt
//
LOCUS       DAZFLD010000352         2746 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.354, whole genome shotgun sequence.
ACCESSION   DAZFLD010000352 DAZFLD010000000
VERSION     DAZFLD010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2746
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.354"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..435)
                     /locus_tag="ACHKBL_004767"
     CDS             complement(<1..435)
                     /locus_tag="ACHKBL_004767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001390888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5821503.1"
                     /translation="MKAENKILNLFHATTMSRGLQIIKDGKIRTNAPSVICWSLETTP
                     GWIYLSDQLDNAVHWGNKIACLSKDELFFYIFRVQVQEENCYPDTDDLQYVSWLSPEE
                     AENTDLYTCLSLCHSCRTDKELYTGCEISDYAVLPATTLYTDN"
     gene            660..>2746
                     /locus_tag="ACHKBL_004768"
     CDS             660..>2746
                     /locus_tag="ACHKBL_004768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFO5821504.1"
                     /translation="MNINRIIFAIEDCISTLSRYSVSAFFPDYCDLHTVIGLDQQDRE
                     RRPWLKAPYIATTKQGYYLSVFEISGALKEMDENPDLTAPGTLESLITALAERMNTAY
                     KNIGHKISFVFERDPERGRNEIEAMLLPQRKSIARTGILLQDVLDEKVTTLTPWLVRE
                     RCWLTVWSSEELLSRTDRKDHQTRIRKLAEHAPPARFAQDPWRWTLSALKIRHDAFLD
                     TLEQALTHDSDGLLVRLMDIHEVGREIRRQLERNSTPAVWQPHLPEDARPAGWRQGED
                     TSVFHAPSLNLQLFTTQPETHGSLIKAGDLWHGMVAITLPPQNLRTFNQLVRDVPRAI
                     PWRIRMDLMPGGMKALGVKKTLLTYSSFISPLRPMYDSVMMLNDINKHDPVCIMTIVA
                     TTWGNSREVCTRNQALLKSALEGWGVCGTTTTFGDPRRAWVNTVLAASHGSGPVPLYP
                     PLSHALSLFPLNRAGSVWRGQGNLMMHTEDGSAWEVALASSQQNKHTELTPGAPGLGK
                     SVLINALSEIQIASAQKNLPFIAYIDKGFSAQGLVQLIRDSLPEQRKDEAVGIILSND
                     PEHTRNLFDVMYGAKKPATPEKNFMVSVLCALCVDTGTGQPCNPGDTRQIISNLVDLA
                     FREYGENNPRLYRAGTEPLVDLALEESGIAEQHDAGWWNAATWFEIRDMLHMAGNIPA
                     AQRAHYQAMPLLA"
BASE COUNT          695 a          785 c          650 g          616 t
ORIGIN      
        1 attgtcagta tacagtgttg ttgcaggaag gactgcataa tctgatattt cacaacctgt
       61 atacagttct ttgtcagttc ggcaggaatg gcaaagagaa agacatgtat acaggtctgt
      121 gttttctgcc tcctcaggag ataaccacga cacatattgc aaatcatctg tatcaggata
      181 acaattttct tcctgaacct gaactctgaa aatatagaaa aataattcat cttttgaaag
      241 gcatgctatt ttattccccc aatgcaccgc attatcaagt tgatccgata aataaatcca
      301 tcctggtgtc gtctctaacg accagcatat aacagaagga gcattagttc ttattttccc
      361 atcttttatt atttgcaggc ctctgctcat tgttgtcgca tgaaaaagat ttaatatttt
      421 attttcagcc ttcactctat ttcctcattt cccaagaaac atattaaaaa cggaaaacat
      481 tacacataac ctggaggaca aatcatgact ttaaatttcc ctgccgggga aactacacta
      541 ttctcagcaa aaaacatcct gacagcaata ctcactctgc acaacttaca cgcatagcgt
      601 tcccatttga atacagttgc agcccgcgat ttattttgtc ttccggagcc acctctgcca
      661 tgaatatcaa ccgcattatt tttgccattg aagactgtat cagtaccctg tcacggtatt
      721 ccgtttccgc attcttcccc gattactgtg atctgcacac cgtcatcggt ctggaccaac
      781 aggaccgcga acgccgcccc tggctgaaag ccccctacat tgccaccacc aaacaggggt
      841 attacctttc cgtctttgaa atcagcggtg ccctgaaaga aatggatgaa aacccggacc
      901 tgacagcccc cggcacactg gaatcactga ttacagcgct ggcagagcga atgaacactg
      961 cctataaaaa catcggtcac aaaatcagtt ttgttttcga acgcgatccg gagcgtggaa
     1021 ggaatgaaat cgaagccatg ctcttacccc agcgaaaatc catcgcacgc acggggatcc
     1081 tgcttcagga cgttctggat gaaaaagtca ccacgctcac cccctggctt gtccgggaac
     1141 gctgctggct gaccgtctgg agctctgaag aactgctgag ccggaccgat cgcaaggacc
     1201 accagacccg tatcaggaaa ctcgctgaac acgctccgcc agcccgattt gcgcaggatc
     1261 catggcgctg gacactttcc gcactcaaaa tacggcatga cgcctttctg gacacgctgg
     1321 agcaggccct gacacatgat tctgatggtc tgctggttcg cctgatggat attcatgaag
     1381 tgggacggga aatccgccgt caactggagc gcaacagcac acctgcagta tggcagcctc
     1441 atctgcctga agatgcccgt cctgccggct ggcgacaggg cgaggatacc tctgtattcc
     1501 acgcaccgtc gctgaatctg cagttattca ccacacagcc tgaaacacac ggcagcctga
     1561 taaaggccgg tgacctctgg cacggtatgg tcgccattac cctgcccccc cagaatctgc
     1621 ggacattcaa ccagctggtc agggatgtgc cccgcgcaat cccctggcgt atccggatgg
     1681 atctgatgcc cggcggcatg aaggcgcttg gggtcaagaa aaccctgctg acctacagca
     1741 gctttatttc ccctctccgg cccatgtatg actcagtgat gatgctgaat gacatcaaca
     1801 aacatgatcc ggtctgtatc atgactattg ttgccaccac atggggaaac agccgggaag
     1861 tctgtacccg caaccaggcg ctcctgaaat ctgcgctgga aggctgggga gtatgcggca
     1921 ccaccaccac cttcggtgat ccgagacgtg cctgggtgaa cacggttctg gcggcctccc
     1981 acggttccgg tccagtcccc ctttacccgc ccctttcaca cgccctttcc ctgtttcccc
     2041 tgaacagggc cggctctgtc tggcggggac agggaaatct gatgatgcac accgaagacg
     2101 gctctgcatg ggaggtcgcg ctggcatcgt cacaacagaa caagcacacg gaactgacgc
     2161 ccggcgcacc cggtctgggc aaatcggtac tcatcaacgc actgtcggag atccagatag
     2221 cctcagccca gaagaacctg cctttcattg cctatatcga caagggattc agtgcacagg
     2281 gactggtaca gcttatccgt gacagcctgc cggagcagag aaaggacgaa gccgtgggga
     2341 tcattctgag caatgatccg gaacacaccc gtaacctgtt tgatgtgatg tacggtgcta
     2401 aaaaacccgc caccccggaa aagaacttca tggtatctgt actctgcgca ctgtgcgtgg
     2461 ataccggcac cggtcagccc tgtaatcccg gtgacacccg gcaaatcata agcaacctgg
     2521 tggatctggc cttcagggag tatggcgaaa acaaccccag actgtaccgt gcaggaacag
     2581 agccccttgt ggatctcgcc ctggaagaga gtggcatcgc agaacaacat gacgctggat
     2641 ggtggaatgc tgccacctgg tttgaaatca gggacatgct gcatatggct ggcaatatcc
     2701 ccgcagccca gcgggcacac taccaggcca tgcccctgct ggctga
//
LOCUS       DAZFLD010000353         2733 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.355, whole genome shotgun sequence.
ACCESSION   DAZFLD010000353 DAZFLD010000000
VERSION     DAZFLD010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2733
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.355"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..442)
                     /locus_tag="ACHKBL_004769"
     CDS             complement(<1..442)
                     /locus_tag="ACHKBL_004769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5821505.1"
                     /translation="MEYGKYETLARYGYTGAARPQGDWQTSAALTRQQYDDWRTRYLP
                     RVARLADLGENNSLMNAQLARVGGLATSSLRTAQMAQDNQMARYGVSRPDNPDSNTLG
                     LRNALAIAGAKNGIREAEQDRQMNILTGASAPARQKLSVGGQLVA"
     gene            complement(445..1101)
                     /locus_tag="ACHKBL_004770"
     CDS             complement(445..1101)
                     /locus_tag="ACHKBL_004770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5821506.1"
                     /translation="MGGSKGGGDTKVKPTAAQIAQEEVAWKGWQDYKNILRPAEDNFM
                     EKVDDLNSEQQYENIAGTTNLGYQKQFGEARKELAGNLAQSGVDPSSGRFNAVMNANQ
                     SDQVTGQIDTTTRGQVSQADKYVAGLQDVAALGSGQKADALQSFNSLADSSLAKAKSD
                     AQAAFTKQQGRASLVGAGLGAAGAYAMHKAGGSGGSGGAKTPGTGANAIQYQAQNWRL
                     "
     gene            complement(1196..1597)
                     /locus_tag="ACHKBL_004771"
     CDS             complement(1196..1597)
                     /locus_tag="ACHKBL_004771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5821507.1"
                     /translation="MAPVSGERMNDKILRYMQRVVRNSRNPEFMNEVKDACLKKQAFC
                     FEAPDGFLVLRSVLSADGIPYVLVLLGVCTGSNSVERYLPEVKTLTRLAGGRWAEFHT
                     ARRGFIRLGKRLGFERMPDDEDGFMVFRIAV"
     gene            complement(1654..1794)
                     /locus_tag="ACHKBL_004772"
     CDS             complement(1654..1794)
                     /locus_tag="ACHKBL_004772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFO5821508.1"
                     /translation="MSQKSLITVTICMTVIFTIWMLHGSLCEFRLNLWGAEFAAFLQC
                     KQ"
     gene            complement(1996..>2733)
                     /locus_tag="ACHKBL_004773"
     CDS             complement(1996..>2733)
                     /locus_tag="ACHKBL_004773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000197193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3672 domain-containing protein"
                     /protein_id="HFO5821509.1"
                     /translation="VKAGVSITSPVIRSAVIQNGNFQVESDGSIKSGEHFSVSVNGDV
                     TASNAKISGHIDADSGTLNNVMIEENCIIKGTMSADRIIGDIVKGYSFEPHDGTLHIT
                     NHKYGEGYVIFKAEVSLADDVSFDRKLILLPFTFYSGTLTIEINGRVVIKADPRVANT
                     GTAGYFDRYDNPVYTRTNLGNTVSGVWDVAAGGGDTRITVTMRVEGGPGNMGDVEGRK
                     RELVRWRYDAFTLMVAKSTDSGFTRIS"
BASE COUNT          625 a          814 c          584 g          710 t
ORIGIN      
        1 ctgccaccag ttgtccgcca acactcagtt tctgtcttgc cggtgcagaa gcccccgtca
       61 gaatattcat ctggcgatcc tgttcggctt cacggatacc atttttcgca cctgctattg
      121 ccagggcatt acgtaacccc agcgtattac tgtcgggatt atccgggcgg cttaccccgt
      181 atctcgccat ctggttatcc tgcgccatct gcgctgtacg gagactggaa gtggcaaggc
      241 ctcccacccg ggcaagctgt gcattcatca ggctgttgtt ctcgccaagg tcagccagcc
      301 ttgctacacg gggtaaatat ctggtgcgcc agtcgtcgta ttgctggcgg gtcagcgctg
      361 cggatgtctg ccagtccccc tgagggcggg ctgctccggt ataaccgtat ctcgcaagtg
      421 tttcgtattt accgtactcc ataatcacag tctccagttc tgagcctgat actgaatggc
      481 attagcgccg gtgccaggtg ttttagcacc gccacttcct ccgctaccac cagccttatg
      541 catcgcatat gcacctgccg cacccaaacc agcgccaaca agagaggctc gcccctgctg
      601 tttcgtaaac gcagcctgtg cgtccgattt agcttttgcc agactgctgt ctgccagaga
      661 gttaaaactc tgtaacgcat ccgccttttg acctgaaccg agagctgcaa catcctgtag
      721 cccggcaaca tacttatctg cctgcgatac ctgcccccgt gtggttgtgt caatctgccc
      781 ggttacctga tcactctggt tcgcattcat taccgcgtta aagcgaccac tggacgggtc
      841 aacaccggac tgagcaagat tacccgccag ctccttccgt gcttcgccaa actgtttctg
      901 ataaccaaga tttgttgtgc cagcgatatt ttcgtactgc tgctcactgt taaggtcatc
      961 gaccttttcc atgaagttat cttctgccgg gcggaggata tttttgtaat cctgccaccc
     1021 tttccaggcc acttcttcct gtgctatttg cgctgctgtt ggttttactt tggtatcacc
     1081 accaccttta cttccaccca taatggcccc ctggataata aaaaaaccct gccggagcag
     1141 ggtcagaatg tgaattgcaa tgttggttta aacacgatga tgaaaactgt ggcagtcaga
     1201 ccgctatcct gaacaccatg aagccatcct catcatccgg cattcgctca aagcccagac
     1261 gttttcccag ccggataaat ccccgccttg ccgtatgaaa ttcagcccag cgtccgccag
     1321 ccagacgggt taatgtcttc acctccggca gataacgctc aacgctgtta ctccccgtac
     1381 acacgcccag caacaccaga acataaggga taccatcagc actgagcaca gaacgcagca
     1441 ccagaaagcc atcaggtgcc tcaaaacaaa acgcctgctt tttaaggcag gcgtctttaa
     1501 cttcattcat aaattcaggg ttgcgggaat ttctcaccac acgctgcata taccggagaa
     1561 ttttatcgtt cattctctca cctgaaacgg gtgccatatc ggctgaacct cagcaaccag
     1621 ttgacgggga ctttcatccc cgtcgcggtt ttcctactgc ttacactgta agaacgccgc
     1681 aaactccgct ccccacaaat tcagccggaa ctcacacagt gaaccgtgca acatccagat
     1741 ggtgaagata accgtcatgc aaattgtgac ggtgatgagc gatttttgcg acataacgct
     1801 tgtctcctga atggagaggc gctaacctac tacttgtgaa ggttgtacgg tcagggcctc
     1861 gggttaacgt taaagttgac tcggggcctt tccacatcag gccttcaggt tcaccctcca
     1921 gccatcagcc gaaaggcacc cacgcatact gtacggtttt tgtctccttc cggcaatccg
     1981 tattacaact gcctgtcacg atatccgggt aaaaccggaa tctgtcgatt tcgccaccat
     2041 gagagtaaag gcgtcatatc tccatcgcac cagttctctc tttctccctt ccacatcccc
     2101 catattgcca ggaccacctt caactctcat tgtcactgta atccgtgtat ctccccctcc
     2161 tgccgccaca tcccatactc ctgaaacggt gttcccgagg ttggtgcgcg tgtaaacagg
     2221 attgtcgtat ctgtcaaaat aaccagccgt tcccgtatta gcaactcttg ggtctgcctt
     2281 aatgacaact cttccgttaa tctctatcgt cagtgttcct gaatagaaag tgaagggaag
     2341 gagtatcaac ttgcgatcaa acgaaacatc atcagcaaga gatacctccg ccttaaagat
     2401 gacataaccc tctccatact tgtggttcgt aatatggagc gtgccatcat gaggttcgaa
     2461 ggaatatccc ttgacgatat ctccgatgat tctgtccgca ctcattgttc ccttaatgat
     2521 gcagttttct tcaatcatca cgttattcag cgtgccggaa tccgcatcaa tatgaccact
     2581 tatcttcgca ttactggccg tcacatctcc attcactgaa acgctgaaat gttccccgct
     2641 ttttatgctg ccatcggact caacctgaaa atttccgttc tgaataacgg cactccggat
     2701 aactggtgaa gtgatactta ccccggcctt tac
//
LOCUS       DAZFLD010000354         2722 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.356, whole genome shotgun sequence.
ACCESSION   DAZFLD010000354 DAZFLD010000000
VERSION     DAZFLD010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2722)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2722)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2722
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.356"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            44..577
                     /locus_tag="ACHKBL_004774"
     CDS             44..577
                     /locus_tag="ACHKBL_004774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFO5821510.1"
                     /translation="MSRKFRYGLSAAVLALIAAGASAPEILDQFLDEKEGNYTTAYRD
                     GAGIWTICRGAIMVDGKPVIPGMKLSKEKCDRVNAIERDKALAWVAKNIRVPLTEPQK
                     AGIASFCPYNIGPGKCFSSTFYRKLNAGDRKGACAEIRRWIYDGGQDCRIRSNNCYGQ
                     VSRRDQESALACWGIDR"
     gene            851..1420
                     /locus_tag="ACHKBL_004775"
     CDS             851..1420
                     /locus_tag="ACHKBL_004775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor N-terminal domain-containing
                     protein"
                     /protein_id="HFO5821511.1"
                     /translation="MNMMTVPFHGDSLYVVNHNGEPYVPMKPVVAGMGLAWQSQLAKL
                     RQRFASTITEIVMVAEDGKRRNMVSLPLRKLAGWLQTINPNKVKPEIRDKVIQYQEEC
                     DDVLYEYWTKGGVVNPRRMSVMEELNQACADMKRDKNIASVFATGLNEWKQVKAAHVS
                     KIRTLINEANLLIDFVLVDTGKGKITKAD"
     gene            1420..1569
                     /locus_tag="ACHKBL_004776"
     CDS             1420..1569
                     /locus_tag="ACHKBL_004776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5821512.1"
                     /translation="MEWWLMISDKIITLTKTICVIVGVSFSLMMVALFLSIAWMALSS
                     VGLVG"
     gene            1620..1688
                     /locus_tag="ACHKBL_004777"
                     /pseudo
     CDS             1620..1688
                     /locus_tag="ACHKBL_004777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309121.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1690..2457
                     /locus_tag="ACHKBL_004778"
     CDS             1690..2457
                     /locus_tag="ACHKBL_004778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000664222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HFO5821513.1"
                     /translation="MNNNSAKPFLPWLGGKRRLAKHILPLFPEHTLYVEPFCGGAALF
                     FMKKPSCAEIINDINSDIVNLYRVVKYHQEEFSRQFQWMLASREMWNEVINQHTEYMT
                     DIQRAVRFFFIQKCAYGGRAYSRAFGVKTHRPQIIPGNTEQLLQEARDRLARATIEHL
                     DWEVCIARYDRPTTLFYCDPPYWLDGGYGIDFSFCNYERMAELARTIKGKMIISLNDT
                     QEMRRLFEGMNIHHVAHRYTVPRGKTSSVSELIICNF"
     gene            2475..>2722
                     /locus_tag="ACHKBL_004779"
     CDS             2475..>2722
                     /locus_tag="ACHKBL_004779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysis system i-spanin subunit Rz"
                     /protein_id="HFO5821514.1"
                     /translation="MNRMLSAFTVILLVACGVLSLGLNHYRDNALTYKAQRDKKASEL
                     ELANATITDMQIRQRDVAALDARYSRELADARAENETLR"
BASE COUNT          718 a          581 c          754 g          669 t
ORIGIN      
        1 attaaagaag accggcgtaa ggcggcgcgg ggagagtaag ctgatgagca ggaaattccg
       61 ctatggttta tcggctgccg ttctggcgct gattgctgca ggtgcttctg cgcctgaaat
      121 cctcgaccag tttctggatg aaaaagaagg taactacacc acggcatacc gtgatggtgc
      181 gggtatctgg accatctgcc ggggtgccat catggtggat ggtaagcctg tgattcctgg
      241 catgaagctg tcgaaggaaa aatgcgaccg ggttaacgct atcgaacggg ataaggcgct
      301 ggcatgggtg gcgaaaaaca tcagagtgcc actgaccgaa cctcagaaag cggggatcgc
      361 gtcattctgc ccttacaaca ttggccccgg aaagtgtttc agctcaacgt tttaccggaa
      421 actgaacgcc ggagaccgta aaggcgcatg tgctgaaatt cgtcgctgga tatatgacgg
      481 tggtcaggac tgccgtatcc gctcaaataa ttgctacggt caggtatccc gtcgcgacca
      541 ggagagtgcg ctggcatgct ggggtatcga caggtagcag aatattttgc tgaaaaatga
      601 cgtttgctca cgcggacgga taacacgaaa tcctgcgaac tggcaaaatg caagtgaata
      661 aagtcaggaa gattgtttca cgcagaggca ccgtaatggt gtctttgtca tttctgcgct
      721 tcgcacaagc gtaaataaac caaagaacct ttcaggatga gccctggtgg ataaccggca
      781 gtggtctggt taaccctctt tgggctggtt attcctgtgc gcagggttca tcactaaaag
      841 gaaataaccg atgaatatga tgaccgtgcc gtttcacggc gattctcttt atgtggttaa
      901 ccataacggt gagccatacg ttcccatgaa acctgtcgtt gcggggatgg ggctggcctg
      961 gcaatcacag ttggctaagt taagacagcg ttttgcgtca actataacgg aaatcgttat
     1021 ggttgctgag gatgggaaac gacgcaatat ggtgtccctg ccacttcgaa aacttgcagg
     1081 ctggttacaa accatcaatc ccaacaaagt aaaacccgaa atccgcgaca aggtaatcca
     1141 gtatcaggaa gagtgcgacg atgttctcta tgaatactgg acgaaggggg gggtcgttaa
     1201 tccccgtcga atgagtgtga tggaagaact caatcaggcc tgcgctgaca tgaaacggga
     1261 taaaaacatt gccagtgtgt ttgctaccgg gctgaatgag tggaaacagg ttaaagccgc
     1321 gcatgtatca aaaatccgca cattgataaa cgaagcgaat ctgctgattg attttgtcct
     1381 ggttgataca ggcaaaggga aaataacaaa ggcggattga tggagtggtg gctaatgata
     1441 tcggataaaa tcataacgct gacaaagacc atttgtgtga ttgtcggcgt ttcattttcg
     1501 ctaatgatgg ttgctctttt tctttccata gcctggatgg cgttgagttc ggtagggctg
     1561 gtggggtgac agtgactgat gacgacaatt tatttaaaca gatgagtact gaatttattc
     1621 gggcaacggc atttgccatt cgcattgtgg caattgcggt tctaatccgg gcaatccgct
     1681 ggtggtgaaa tgaataataa ctctgcaaaa ccgtttctcc cgtggttagg agggaagcgt
     1741 cgtctggcaa aacatattct gccgttattt cctgagcaca ctctgtatgt ggaaccgttc
     1801 tgtggcggtg cggcactgtt ctttatgaaa aaaccgagct gcgctgaaat catcaacgat
     1861 ataaacagtg atatcgttaa tctctaccgt gtggtgaagt accaccagga ggaattttcc
     1921 cgccagtttc agtggatgct cgccagtcgt gagatgtgga atgaggtgat aaatcagcac
     1981 acggagtaca tgacggatat ccagcgggct gtccgtttct tttttatcca gaagtgcgcg
     2041 tatggcggaa gagcatacag tcgggcattt ggagttaaaa ctcacagacc gcagataatc
     2101 cccgggaata cagagcagtt attgcaggaa gcccgtgacc ggctggcgcg ggcaactatt
     2161 gaacaccttg actgggaggt ctgtatcgca cggtatgatc gcccgacaac gctgttttac
     2221 tgcgatcctc cgtattggtt ggatggtggt tacggcattg atttttcatt ctgcaattat
     2281 gagcgcatgg cagaacttgc ccgcacaata aaaggcaaaa tgattatatc cctgaacgat
     2341 acgcaggaaa tgagacgatt gtttgagggg atgaatattc atcatgttgc tcatcgttat
     2401 actgtaccac gtggtaagac aagcagtgtc agcgagctga taatctgcaa tttctgacag
     2461 ggggcatggt gagcataaac cgaatgcttt ccgcgtttac cgttattctg ctggtagcct
     2521 gtggtgtgct tagtctgggg ctgaatcatt accgtgacaa cgcgctcacc tacaaagcgc
     2581 agcgcgataa aaaagccagt gagctggagc tggcgaacgc gacaattact gatatgcaga
     2641 tacgccagcg tgatgtcgct gcacttgatg ccagatactc gagggaatta gccgatgcga
     2701 gagctgaaaa tgaaactctg cg
//
LOCUS       DAZFLD010000355         2722 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.357, whole genome shotgun sequence.
ACCESSION   DAZFLD010000355 DAZFLD010000000
VERSION     DAZFLD010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2722)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2722)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2722
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.357"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            336..1019
                     /locus_tag="ACHKBL_004780"
     CDS             336..1019
                     /locus_tag="ACHKBL_004780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HFO5821515.1"
                     /translation="MNLKKTLLSVLIIVPLCLLAGCDYIEKASKVDDLVTQQELQKSK
                     IEALEKQQELDKRKIEHFEKQQTTIINSTKTLAGVVKAVKDKQDEFVFTEFNPAQTQY
                     FILNNGSVGLAGRVLAIDAVENGSVIRISLVNLLSVPVSNIGFHATWGNERPTDAKAL
                     AKWQQLLFNTTLNSTLQLMPGQWQDINLTLKGVSPNNLKYLKLSINMANLQFDTVQSA
                     ETRQRKNKK"
     gene            1063..>2722
                     /locus_tag="ACHKBL_004781"
     CDS             1063..>2722
                     /locus_tag="ACHKBL_004781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001696305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ESPR-type extended signal peptide-containing
                     protein"
                     /protein_id="HFO5821516.1"
                     /translation="MNKIFKVIWNPATGSYSVASETAKSRGKKSGRSKLLISVLVASG
                     LLSSFGALANNYDGQPTDYGDGSAGDGWVAIGKGAKANTFMNTSGASTALGYDAIAEG
                     EYSSAIGSKTLATGGASMAFGVSARAMGDRSVALGASSVANGDRSMAFGRYAKTNGYT
                     SLAIGDSSLADGEKTIALGNTAKAYEIMSIALGDHANASKEYSMALGASSAASAANAI
                     AVGRNSAAAGVDSLAFGRLSAANAANAIAMGAESKAAENATAVGTNAEANGLNSIALG
                     SGSIADVDNTIALGNQSQAVAAGAIAIGQGNKADGANAIALGNGSITGGVNAIALGQG
                     SYAGLENGTAIGAQASAQGKNSVALGAGSVATDADTVSVGNTTAQRQIVNMAAGDIST
                     TSTDAINGSQLYAISKSVADNLGGGATVNAQGVVTSPNYRLKSGIFGTVGDALTGLDN
                     NTLQWDSLKKAYSAAHGTDTTSTITNVKDGAISDTSKDAVNGSQLKTTNDNVATNTAN
                     ITTNTNSINTLTDSVGDLKDDALLWNGTAFSAAHGTEATSKITNV"
BASE COUNT          808 a          593 c          653 g          668 t
ORIGIN      
        1 ttgaaacata attaattatt taaatttttc tatcagcatc gcaaacttcg caataaaaca
       61 tcactcaact gaatatttac ctttccctgc cctgggcctt tatccggtga aacatttgac
      121 attgattgac accgatcatt aaaatccgcg cgattaatat tattggctac aaaccgaaat
      181 tgtttgacac ttaataacat attcgcacaa ccgattaata caaaaattct ctaacaaatt
      241 tatttgtcgg atatgctcca ctctaatcat tcataaaaaa aatgattgtg ccgtaattat
      301 caactgtatc gggattatga aaaggaattc atcctatgaa cctaaagaaa acactgttaa
      361 gcgtgttaat aatagtgcct ctctgcttat tggcagggtg tgactatatt gaaaaagcga
      421 gtaaggtcga cgacctcgtt acacagcaag agttgcaaaa aagcaaaatt gaggcgcttg
      481 agaaacaaca ggaactcgat aagcgaaaga tagaacattt tgaaaaacaa caaactacta
      541 tcataaatag taccaagacg ctcgctggcg tggtgaaggc ggttaaagac aaacaggacg
      601 aatttgtctt tacagaattt aacccggcac aaacccaata cttcatttta aataacggct
      661 ctgttggttt ggcaggccgg gtgctggcta ttgatgcagt tgaaaatggc agcgttattc
      721 gtatttcgct ggttaattta ttaagcgttc ccgtatcaaa tattggcttt catgcgacat
      781 ggggcaatga aaggcccacc gatgccaaag ctctggcaaa gtggcaacaa ttacttttca
      841 acaccacact gaattccact ctgcaattaa tgcctggtca atggcaagac attaatctga
      901 cattaaaagg cgtttcgcct aataacctga agtatctgaa gttgtccatc aatatggcca
      961 atctccagtt cgacactgtt caatctgctg aaactcggca gcgaaaaaac aaaaaataaa
     1021 tgcagcttct gcgcttatat caaggaatag agagcatcaa taatgaacaa aatttttaaa
     1081 gttatctgga atccagcgac gggcagttac agcgttgcca gcgaaacggc gaagagccgt
     1141 ggtaaaaaaa gcgggcgcag taagctgtta atttctgtac tagttgcgag tgggttgttg
     1201 tcgtcgtttg gagcgttagc taataactac gacgggcagc caactgatta tggcgatggc
     1261 tcagcaggtg acggctgggt tgctatcggt aaaggggcaa aagcaaatac ctttatgaac
     1321 actagtggcg cgagtacagc tttaggatat gacgcgatag ccgaaggtga gtacagttct
     1381 gccatcgggt caaaaaccct tgcaactggt ggagcatcca tggcgttcgg ggttagtgca
     1441 agagcaatgg gtgacagaag tgtcgcgctg ggtgcatcgt cagtagcaaa tggcgatcgt
     1501 tcgatggctt ttggtcgtta cgcaaagacg aatggttata catctcttgc tattggggac
     1561 tcctcccttg ccgatggtga aaaaactatt gcgttaggaa atacggctaa agcttacgaa
     1621 attatgagca tcgccctcgg tgatcatgcc aatgcgtcaa aagaatattc aatggcgctg
     1681 ggtgcaagta gtgccgcatc tgcggctaac gcgattgcag taggccgcaa cagtgctgca
     1741 gctggcgtag acagcctcgc attcggtaga ctatccgccg ccaacgccgc aaacgcgatt
     1801 gctatgggtg cagagagcaa agctgctgaa aatgcgacgg cagttggtac taacgcggag
     1861 gcaaatggac ttaatagtat cgcgttgggc tccggcagta ttgcagatgt cgacaacact
     1921 attgctctgg gcaatcaaag tcaggcagta gcggctggcg caattgccat cggtcaaggg
     1981 aataaagctg acggcgcaaa tgctatcgcg ctgggtaatg gtagtattac aggtggtgta
     2041 aatgctattg ctcttggaca aggcagttat gccggtttag aaaatggcac tgcaattggt
     2101 gctcaagcca gtgctcaggg gaaaaattca gttgctctgg gtgctggttc tgtagcgact
     2161 gacgcggata ctgtttctgt gggtaacaca acagctcagc gacaaattgt caatatggca
     2221 gcaggtgata tcagcactac cagtactgat gccatcaatg gatcacagct ttatgctatc
     2281 agtaagtcag tagcggacaa tcttggtggg ggggctaccg tcaatgcgca aggcgtcgtt
     2341 acttccccaa attacaggct gaaaagtggt attttcggca ctgttggcga cgccttaacg
     2401 ggcctggaca ataatacgtt acaatgggac tcccttaaaa aggcatatag tgcggcacat
     2461 ggtacagata ctaccagtac catcaccaac gttaaagacg gcgctatttc tgataccagt
     2521 aaggatgcgg ttaacggttc tcagctgaaa accaccaacg ataacgtagc gaccaatact
     2581 gccaatatca ccaccaacac taacagtatc aataccctga cggattccgt tggcgacctt
     2641 aaagacgatg ccctgctgtg gaatggcacc gcgttcagcg ccgcgcacgg caccgaagcc
     2701 accagcaaaa tcaccaacgt ca
//
LOCUS       DAZFLD010000356         2717 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.358, whole genome shotgun sequence.
ACCESSION   DAZFLD010000356 DAZFLD010000000
VERSION     DAZFLD010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2717
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.358"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            847..1845
                     /locus_tag="ACHKBL_004782"
     CDS             847..1845
                     /locus_tag="ACHKBL_004782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HFO5821517.1"
                     /translation="MKNPELKNLTDYSPEDKPWDERRSFSDDVGGIYLRAAGFEHYGE
                     RVGACSGILRFGWSTNTETGETRLRLRDARFCRVRHCPICQWRRSMMWQARFYQSLPK
                     IVAEYPGARWLFLTLTVRNCSIDALGETLTAMNAGFLNLKRRKEFRGVLGWVRTTEVT
                     RGKDGSAHPHFHTLLMVSPSMLSGREYVKHARWVELWRDCLRVAYDPNVDIRVVKSRK
                     PKDDESLPCVVAEQLRGAVVETLKYSTKPADMLADPEWFLELTRQVHKRRFVATGGAL
                     KNVLQLERETNEDLIMADGMAEGTDDGSRLAFSWVPVVKKYQRDRAKDKRRLSSDD"
     gene            complement(1828..2265)
                     /locus_tag="ACHKBL_004783"
     CDS             complement(1828..2265)
                     /locus_tag="ACHKBL_004783"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5821518.1"
                     /translation="MAVAASARNALKIIRCTANKKLSSAKNHVKTIASTFLSPSITVK
                     HFASVGKLLTSIIQFLIDNNSGDIPSFEGLFLPTHHKQQPYHRQKRAQNASDGMNQPK
                     HTKPLLNAKKCVRKRLSAFLESKKLSAVDFHCRDPETRSIIAA"
     gene            2505..>2717
                     /locus_tag="ACHKBL_004784"
     CDS             2505..>2717
                     /locus_tag="ACHKBL_004784"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5821519.1"
                     /translation="MSPERWRNVGSFCAAPPSTEARSSGNRTANHSAVFVPQLFRAID
                     KLAVGESHEVRTYTISTSFRLRAVCDA"
BASE COUNT          608 a          604 c          803 g          702 t
ORIGIN      
        1 aaaaaaaccg agaaatggcg gtggttttcg ggacaggatg tgtatttgtc ggaccgcgca
       61 cacgcaatca gacaaatgcc gcctggtcag gactgcgccc cgacaccccg ccaacgtctc
      121 acgcagccag attgaaatct gtcagcgttc gccgttttat gactcgtcaa acagaaaatc
      181 gtggggtccc gcgcctccgg cgtgggttcc gattttgtga ttgacgacat ggcggataaa
      241 aacactggtg gtggtggttg gtgtcacgca gtgaggccaa ccacctgcaa agcgggtttt
      301 taggaggatg ggaattgaaa aatatctatc agctatacga ggaaaatcgc aacagagccg
      361 gatttaaggt tcggcggaca tcatggggga gaactgttgc ggttattaaa tccattggtg
      421 gaaagactcg cgggaagctt tcgggttatg cgccttacta cggcaatcaa agggttgtgg
      481 ctgagattca tacgccgagc ggtattatta cggatgttct ttcgtgtgct gggtgctacc
      541 agtgggagcg cgtggattga tgtgttttta ggtggttgcc accttgtata tatacttaag
      601 ggataaatcg acaactcgaa atcatgctct aggccttgtc acgactggcc tgaacgtgga
      661 tttgcgtgaa aataggtttg agagtatttt gtcggaagtg gtcgcattaa aagcgacccc
      721 cgcaacgatg ggcgtcgttc gggggtgtga ccacctgtag gaattcgaat tggtgacacg
      781 acaacacagg tgatacagga atgataacac gtaatttcgt ctgtcgctca ctagggaacg
      841 gcagacatga aaaatcctga acttaaaaat ctgacggatt attccccgga ggataaaccg
      901 tgggatgagc gccggagctt ttccgacgac gtgggcggta tttatctgcg tgctgccgga
      961 tttgagcact acggcgaacg tgtcggggct tgtagtggta ttttgcggtt tgggtggtca
     1021 acaaacacag agaccggaga aacgcgcctg cggttgcgtg atgcgcggtt ctgtcgtgtc
     1081 cggcactgtc cgatctgcca gtggaggcgc tccatgatgt ggcaagcccg tttctatcag
     1141 tccctgccaa agattgtggc ggaatatcca ggcgcgcgct ggttgtttct cacgctgacg
     1201 gtgcgcaact gttccattga tgcgcttggt gagactttga ccgcgatgaa tgcgggtttt
     1261 ttgaatctca agcgtcgcaa ggagtttcgt ggtgtgctgg gctgggttcg taccacggaa
     1321 gtaacgcgcg gcaaggatgg tagcgcacat ccccattttc acactctgct gatggtttcc
     1381 ccctcgatgt tgtccggccg tgagtatgtg aaacatgccc gttgggtgga actgtggcgt
     1441 gattgtctgc gggtggcgta tgacccgaat gtggatatca gagtggtgaa gtcacggaaa
     1501 ccaaaagacg atgaatcttt accttgtgtt gttgcggaac agctgcgcgg cgcggtggtt
     1561 gaaactctga aatattccac aaagcctgct gatatgctgg ctgatcctga atggttcctc
     1621 gaattaacac ggcaggtaca caagcgtcgt tttgttgcta cggggggagc gcttaagaac
     1681 gttctgcaac tggagcggga aacgaacgag gatttaatca tggcggatgg tatggcagag
     1741 ggtacagatg acggttcccg tctggcgttt tcgtgggtgc cggttgtcaa aaaataccag
     1801 cgcgatcggg cgaaagataa acgtcggcta agcagcgatg attgagcgcg tttctgggtc
     1861 tctgcaatgg aaatccacgg ctgacagctt tttgctctcc agaaacgcgc tcagacgctt
     1921 tctaacgcac ttttttgcat tgagcaatgg ttttgtatgt tttggttggt tcatgccgtc
     1981 agaggcgttt tgagcgcgtt tttgacgatg gtatggctgc tgtttgtggt gtgtgggtaa
     2041 aaaaagcccc tcaaaagagg ggatatcacc ggaattattg tcaatgagaa attgaattat
     2101 ggaggtgagc aacttcccga cagatgcaaa atgtttgacc gtaattgaag gagacaggaa
     2161 ggtgctcgcg attgttttaa cgtgattttt tgcagatgac aattttttgt tcgctgtaca
     2221 tctgatgatt ttgagtgcgt ttctggcgct tgctgctact gccaaaccat ccccatggtc
     2281 agttgatgcc agaaacgcgc agaagttttg tagcgcgtct ccggtcccta atcaatgaat
     2341 tcccggtcat taacatgcgt ttacccggaa acgcgccctg atgttctatc gtattgcggg
     2401 aaaggtgtcg agggtgtcgg gcaaagccct gaccaggtgg taatcgtata gccgtgcgtg
     2461 cgcggttata cgattacaca tcctgtcccg atttctgcgg gtttgtgtca ccagaacgct
     2521 ggcgcaatgt cggcagtttt tgcgccgcgc cgccctccac ggaagcgcgc agttctggca
     2581 accggacagc aaatcattct gccgttttcg tgccgcagct gttccgcgcg atcgacaaac
     2641 tggcagtcgg ggaatctcac gaagtgcgca cttatacaat ctctacttcg tttcgattgc
     2701 gtgcggtctg cgacgct
//
LOCUS       DAZFLD010000357         2696 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.359, whole genome shotgun sequence.
ACCESSION   DAZFLD010000357 DAZFLD010000000
VERSION     DAZFLD010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2696
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.359"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(737..2674)
                     /gene="iolC"
                     /locus_tag="ACHKBL_004785"
     CDS             complement(737..2674)
                     /gene="iolC"
                     /locus_tag="ACHKBL_004785"
                     /EC_number="2.7.1.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_453701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047590 - 5-dehydro-2-deoxygluconokinase
                     activity [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-2-deoxygluconokinase"
                     /protein_id="HFO5821520.1"
                     /translation="MSMNNTVSSEQKTLDIICLGRVAVDLYGQQIGSRLEDMTTFAKY
                     LGGSSGNVAYGTAIQGLRSAMLARVGDEHMGRFIREELNRVGVNTQCLLTDPERLTAL
                     VILGIKDQDTFPLIFYRDNCADMALAPEDIHETFIASSRALAVTGTHLSHINTRAAVL
                     KALTYAQKHGLRRVLDIDYRPVLWGLTSLGDGETRFIASPQVTEQLQQVLHHFDLIVG
                     TEEEFHIAGGSTDTLTALRRVRQLTSAVLVCKCGALGCSVFEGNIADDWSQVQMHSGV
                     RVDILNVLGAGDAFMSGLLRGYLNDEGWEQACRYANACGALVVSRHGCAPAMPTKQEL
                     DDYLSREHAITRPDKDPHLNHLHRTTTRKQHWPELCVFAFDHRNQLVDIARATGASEF
                     TIPELKMLLLEGARQAAHEAGLLHNCGILADTTFGQKALNDVTGQGWWIGRPIEMPGS
                     YPLKLEHGNIGSQLVSWPKEHVVKCLVFYHPLDAESLRLKQEALIDEIYRACCQSGHD
                     LLLEVILPRDAADQSEHYYTQIVERFYLLGIQPDWWKLPPLSPARWQEISQHIERYDP
                     ECRGILILGLDAPESELKSGFAAAADIPWVKGFAVGRTIFGEPSRQWFSGQINDEQLI
                     AAVKQKYRTLIDYWREYRPAL"
BASE COUNT          694 a          673 c          697 g          632 t
ORIGIN      
        1 ttaaagtatg ttgtagagaa aagtttgtct gaagtggcct actgaaatga attgcgcagg
       61 aaagtgagac ggaagatcgg tcagaccgac accagcaaaa tatgatatat ccgacgacca
      121 tcaaaggtca taaaggcgat aaggaatagg tgcgcagata tccgtcatgg ctctgcaaaa
      181 aactgtaaaa tgaagccgaa tgtacggggg cagtctgaac cccaactgaa gcatcttttt
      241 tgcacaaaat cgggagtcca tgtacggata taaagctgca acctaccgtc aatgtcagaa
      301 caatggaagc tgtgaaaaac gggatgcgtt aatataaact ccatgacagt agtggaacgg
      361 gttaatcaag caggattacc agcaagatga gggagctgat tcagcacagg cgacctgacc
      421 ctggccacgg ataaccgtct gtcgcccgtc tgaggtgtcg ttttttcgtt ctgccacccc
      481 tcggttgagt aaaatggaaa ttaaccgtca agctgcctgt agcgtaccga tcagaagatt
      541 gctcagatgg tacagctgtt gtacgttctg cggcaacgtg acgagggaac taccaatagc
      601 cagacagttc agaaatatca ttaatgcaga tacgaaacag cactgtcggg atgatgagga
      661 aagtaggacg gtcatgaaac atctttgaat acgccccgag caagaataca gacgcgggac
      721 gtaatgcaga cggatactaa agcgccggac gatattcgcg ccagtagtcg atcaatgtgc
      781 ggtatttttg tttcactgct gcgatgagct gttcatcgtt aatctggccg ctgaaccact
      841 gccgtgatgg ctcgccaaag atggtgcgac ctacggcgaa gcctttaacc catggtatat
      901 ccgcagcggc ggcaaagcca gatttcagct cgctttccgg tgcatccagt ccaagtatta
      961 aaataccgcg gcattcggga tcgtagcgct caatatgctg gctgatttct tgccaacgag
     1021 ccggacttaa cggtggaagc ttccaccagt caggctggat ccccagcagg taaaaacgtt
     1081 ccacaatctg tgtatagtaa tgctcgcttt gatctgctgc gtcgcgtggc aagatgacct
     1141 ccagcagcag atcgtgtcca gactggcagc aggcccggta tatctcatca atgagtgctt
     1201 cttgcttaag gcggagggat tctgcatcca gtgggtgata gaagacaagg catttcacga
     1261 cgtgctcttt cggccaactg acaagttgtg aaccgatatt accgtgctcc agcttcagag
     1321 gatatgaccc cggcatttcg ataggacgtc caatccacca tccctgacca gtcacgtcgt
     1381 taagtgcttt ctgaccaaaa gtagtatccg ccaggatgcc gcagttatgc agtaatccag
     1441 cctcgtgcgc cgcctgacgt gcgccttcca gtagtagcat cttcagctca gggatagtga
     1501 actcgctggc acctgtcgct ctggcgatat ccaccagttg attgcgatgg tcaaaggcga
     1561 atacacacag ttccggccag tgctgttttc gggttgtcgt tcggtgaaga tggttaaggt
     1621 gagggtcttt gtcgggtctg gtgatagcgt gctcacggga gagatagtca tccagttcct
     1681 gtttggttgg catcgcgggg gcgcagccgt ggcgcgatac aactaatgcg ccacatgcgt
     1741 tagcataacg acaagcttgc tcccaacctt catcgttcag atagcctcga agcagtcccg
     1801 acataaaggc gtctcctgca cccagcacat ttaggatgtc cacacgtaca ccgctgtgca
     1861 tctgcacctg agaccagtcg tcggcgatgt ttccttcaaa tactgaacac cctaatgcgc
     1921 cgcatttgca taccaatacc gcagaggtta gctgacgcac acggcgcagg gcggtcaacg
     1981 tatcagtact gccgccggcg atatgaaatt cctcttcggt gccaactatc agatcaaaat
     2041 ggtgcaatac ctgttgaagc tgttcagtca cctgtggtga agcgatgaaa cgtgtttcac
     2101 catcccccag cgatgtcaac ccccatagta ccggacgata atcaatatcc agcactctac
     2161 gcagaccgtg tttttgagca taggtcagcg ctttaagcac tgcggcgcgg gtattgatgt
     2221 gagaaagatg agtgccggtg acggccaggg cacgtgaaga ggcaatgaat gtttcgtgga
     2281 tatcctcagg cgccagcgcc atatccgcac agttatctcg ataaaagatc agcgggaagg
     2341 tgtcctgatc tttaatacca aggatcacca gggctgtcag acgttcagga tcagtcaaca
     2401 ggcattgtgt attgacgcca acacggttta attcttcgcg gatgaaacgt cccatatgtt
     2461 catcgcccac acgtgccagc atggcggaac gtagtccctg aattgctgtg ccgtaagcga
     2521 cgttgcctga tgagcctcct aaatattttg caaaggttgt catgtcttcc agacgtgacc
     2581 cgatttgttg accatataaa tcaacggcga cacgccccag acagataatg tccagcgttt
     2641 tttgctcact gctgactgtg ttgttcatag agatatcctc ctacagaaga ccgtgt
//
LOCUS       DAZFLD010000358         2657 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.360, whole genome shotgun sequence.
ACCESSION   DAZFLD010000358 DAZFLD010000000
VERSION     DAZFLD010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2657
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.360"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            541..933
                     /locus_tag="ACHKBL_004786"
     CDS             541..933
                     /locus_tag="ACHKBL_004786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001694413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HFO5821521.1"
                     /translation="MRDIQQVLERWGAWAANNRENVTWSSVAAGFKGIIPLKITSRPQ
                     CCDDDAMVICGCIARLYRNNRDLHDLLVDYYVLGKTFMALAHQHGCSDGHIGKRLQKA
                     EGVVEGMLMMLDVRLEMDRHVERESVTA"
     gene            complement(1769..2143)
                     /locus_tag="ACHKBL_004787"
     CDS             complement(1769..2143)
                     /locus_tag="ACHKBL_004787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5821522.1"
                     /translation="MDTHDKIPPAYSYSPQPVPEAWKMRFSANSAWFFTDDFSVVNAW
                     LKAHSDGVEVVPLFCGSDSDGKRDTAFEEVCAALARMLHMHRLLLEKTRYQEHVTDPE
                     CLRELHEAPIQALNILVKHKLS"
BASE COUNT          752 a          606 c          669 g          630 t
ORIGIN      
        1 gccggacagc gtgagcgctg aaaatcaggc tgatgtgacg tgatggtgtc gaactgagta
       61 gctttaagcg actctgtgac tggttttaaa cgagtcgcaa aaagagccgg agaaaaacca
      121 cgaatctggg gttttttcta agcacgctgt acacggtgga acatatggtg gtcaggagac
      181 ggcaggagca acgccagaac gcgcccccgt accagtggca taagccggag cggttacggc
      241 ggtaacttgt tgattaaaag gcggctcaat tctgagccgg gtagctgacg gcgttaactg
      301 gctgatattg ttacaaagct catttttgag ctttggccta acctactgat attttcggaa
      361 acctcaattt gaggagaccg caaacgcaaa attgcgctgg ctacattcct cacttctgag
      421 gactgttaac aagctggctg aaagacagtc acaagctacg aaattttcgt agtttaactt
      481 tcatccagtg catccctgaa ttacgggtat gtaaccacag ctattacgag gtgattgttg
      541 atgcgtgata ttcagcaggt tctggaacgc tggggagcgt gggcggcaaa taatcgcgag
      601 aatgtgacct ggtcatccgt tgctgccgga tttaagggga ttattccgct aaagataacg
      661 tcacgtccgc agtgttgtga cgatgacgcg atggtgatat gcgggtgtat agcccgcctt
      721 taccggaaca accgcgatct gcatgacttg ctggtggatt actacgtgct ggggaaaacg
      781 tttatggcgc tggcacatca acacggatgc tctgatggac atatcggaaa gcggctacag
      841 aaagcggaag gtgttgttga aggcatgctg atgatgctgg atgttcggct tgagatggat
      901 cggcatgttg agcgtgaatc agtgacggca taacgagcat aaaaaaaacc gcagctatga
      961 gaactgcggg aaaggtcgta tcagcatcga gtatcagaga tctcaaacaa tgagattgtg
     1021 ttcattttaa caccacacca aaacatggaa cagccttacg aaaatttcgt aaccctcgca
     1081 tcgtcacata tacctgaatg gtcatgatga ctacgcagac catccaggca accataacga
     1141 acatcgttac agttggagct tttaccccgt tggggggaaa ggtgcacatc tggatcatca
     1201 ttacggtgat caagggtatg taaaacccac cagcctgatt aacggttaac cggaaaaaag
     1261 ccaggtagcc ccgttatggg tattcccata tcgggagcat tcggaactat gcaaatcagc
     1321 acagaaaggg gatggcctga tataatgctc atacctaccg gaacaaggaa ggctggccta
     1381 cgaaaatttc gtaaccctct cccgcacaat gtattaaata atcactacaa gccattgatt
     1441 aaacttaatt ataacgattc gttagaatta gccccgcagg aacaaggaaa gaagaatacg
     1501 aaaatttcgt aaaatcctcc gcacatcccc ccacggttac ccgttcagtg caagcgcaca
     1561 ggcacgacct cgttaaccag ttaccagcgc tcacgctcaa atatccacag gaacggggat
     1621 aaatcgcatt ctggcgcaat gttcactgtc ggggcgagca ttcaccggaa aacgtcatac
     1681 aggcttacag tcggcttaaa attagatgga cggatgacag ggcgggatgg tgagacctac
     1741 acaaaaagtg taggtctcca tgacacggtc aggacagttt gtgtttaacc agaatattta
     1801 gcgcctgtat cggggcttcg tggagttccc gcagacattc agggtcagtt acatgttcct
     1861 ggtatcttgt tttctcaagt aacaagcggt gcatatgcag catacgggca agagccgcgc
     1921 acacctcctc aaatgcggta tcccgcttac cgtcactgtc agagccacaa aacagcggta
     1981 caacctccac gccgtcgctg tgggccttta gccaggcatt aaccacgctg aaatcatcgg
     2041 tgaagaacca cgcactgtta gcgctgaatc gcattttcca ggcttccggt actggttgcg
     2101 gggagtatga atatgctggt ggtatcttgt cgtgagtatc cataggctat cgttccgtca
     2161 ttctgttatt caccacggcg ctaaaaacgc catgaaggac cagaataccg attgcctcgt
     2221 ttgctgtatg aggtgcttac cacacattgg gtagacggat gtatcaggcg gaagtgtaat
     2281 taaccggaaa aaatcccttt cagtctgacg ggcaaaaaaa accgtagtgc gataacacta
     2341 cggcatgcca ccaaagaaac acaacaagag gtcaacaatg agtaatggac atgaccagac
     2401 gattttatca tgccggaaca aaagttatgt gacacagtcg gataaaaaca ggtaaagccc
     2461 accagcttca taggggataa ccggaaaaat tccggtaaat atttgcctga gtaagtcatc
     2521 aagcgcctgc taaatgtgta tagaaatgtg tataggattg attttagaac atgtggaaat
     2581 ggctttatta ctggtggcat ggcttgttgt tctgttccct tcgccgctcc aatttatctt
     2641 cacatcatct acctgcc
//
LOCUS       DAZFLD010000359         2623 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.361, whole genome shotgun sequence.
ACCESSION   DAZFLD010000359 DAZFLD010000000
VERSION     DAZFLD010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2623
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.361"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1281
                     /locus_tag="ACHKBL_004788"
     CDS             <1..1281
                     /locus_tag="ACHKBL_004788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HFO5821523.1"
                     /translation="QMRKISVSYICKSLFLYCSASLPVLSANGIPDKMIVELGDNSAA
                     KANFAVAVGQYSQANAEKSISLGASARAIENNSTALGANATTLKPFSVALGAGSVADR
                     GAGIEAYMPETKRWNPGDVVKSRMSDKQLNRLMEIDKLISAYNSQETLSAEETKQLEE
                     VKKERADMFATYIATHDAVSVGVGATTTGRHAVTRQITSVAAGSVDTDAVNVAQLRDL
                     QTVTEKADAALDKRITNNTSAITKNLQVVNSNKEEISNINKTTKILDERVTANTQTAE
                     EHKLLISENSKQISTNTLGIRENTQRVQDEGQARIRGDRETLRAATGYTDMRVNGLER
                     NTNKRFAQLKEDIDKNRKRADAGIAGAMAMTGIPSVPGKKVSFGMAMAGYRSEGAVAA
                     GFHFNTSENSAVKVNAAWDTENGAGVSAGMALGW"
     gene            complement(1711..2286)
                     /gene="dolP"
                     /locus_tag="ACHKBL_004789"
     CDS             complement(1711..2286)
                     /gene="dolP"
                     /locus_tag="ACHKBL_004789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001238641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006970 - response to osmotic stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/outer membrane stress-associated
                     lipid-binding lipoprotein"
                     /protein_id="HFO5821524.1"
                     /translation="MRLLYSAVVLISALLLQGCVAATVVGTAAVGTKAATDPRSVGTQ
                     VDDEVLELRVSSALSKDEQIRKEARISEIAYQGRVLLIGQSPDRELSARARQIALGVD
                     GVQEVYNEIRQGTPVSPVIMAGDAWLTTKIRSQLLTSARVKSSSVKVVTENSEVFLLG
                     LVTEQEGRAAADIASRVSGVKRVTTAFSCIR"
BASE COUNT          763 a          596 c          631 g          633 t
ORIGIN      
        1 caaatgcgta aaatcagtgt cagttatata tgcaaatcat tatttctcta ctgctcagct
       61 tctttacctg tcttatctgc aaatggtatt cctgataaaa tgatcgtgga acttggtgat
      121 aacagtgcgg caaaagctaa tttcgctgtt gctgttggtc aatattcaca ggcaaatgca
      181 gaaaaaagta tttctttagg tgcatcagca agagctattg aaaataactc taccgcgtta
      241 ggtgcaaacg caacaacatt gaaaccattc tctgtcgctc tgggcgcagg aagtgttgct
      301 gatcgtggcg ctggtattga agcatatatg ccagagacaa agcgctggaa tccaggggat
      361 gtagtaaaat ccaggatgtc tgacaagcag cttaacaggc ttatggaaat cgataagcta
      421 atatccgctt ataattcaca agagacgctg tctgctgaag agactaaaca actggaggaa
      481 gtgaaaaaag agcgggctga tatgtttgcg acatatatag caacgcacga tgctgtatct
      541 gtaggtgttg gtgcaacgac aacaggacga catgctgtta caagacagat aactagcgtt
      601 gcagcaggtt ctgtcgatac ggatgctgtt aacgttgcac agctcaggga tttgcagact
      661 gtgacagaaa aggcagatgc agcgcttgat aaacgcataa caaacaacac cagtgcgata
      721 actaaaaatc tgcaggtggt taacagtaat aaagaggaaa tcagtaacat taataaaacg
      781 acaaaaattc tggatgaaag agtgacagcc aatacacaga cagcagaaga acataagctg
      841 cttatttcgg agaacagcaa acaaatcagt accaacactc ttggtatcag agagaataca
      901 caacgggtcc aggatgaagg acaggctcga atccggggag acagggaaac actgcgtgca
      961 gcgaccggtt ataccgacat gcgggtaaac gggctggagc gtaacacgaa taaacgtttt
     1021 gcacagctta aagaggatat agacaaaaac agaaaacgtg cagatgcagg tattgcaggt
     1081 gcgatggcaa tgacggggat accgtccgtc cccgggaaaa aggtctcatt tggtatggcc
     1141 atggccggat accggagtga aggtgctgtt gctgccggct tccattttaa tacaagtgaa
     1201 aacagtgctg ttaaagtgaa tgcagcctgg gacactgaaa atggagctgg cgtttcagcc
     1261 gggatggctc tgggatggta atgcttccgg gccagaagcc gttcgcagat attgttatcc
     1321 aggggacact gcaccaccat tcaggaagcg attcagttta tcgcatcggc tcaggatgta
     1381 attaatacgg acaatctggc tcctgcaccg gagccagacg tacacgtgtc aacgccacga
     1441 tctttgacct gtatggataa aagctcagat tatttaccag cagcgacatc cggagagcac
     1501 cgggatttta tctgtgagtg aacatacccg ggcaaaaaaa tcccccgaat tcaggggggt
     1561 gtcaaagagg aattatgtat attggttatt ataatgagca cgtctccggt attccgcgac
     1621 gaatgttcac gtggtggcat gaacggcccg gaaacgtgct cttctgattt ttaacgtaaa
     1681 tttgcactaa aggcaactca gatacggcct tcaccgtata caggagaatg ccgtcgtgac
     1741 ccgcttcacg ccactcaccc tactggcaat atctgctgcc gctcgccctt cctgctctgt
     1801 aaccaggccc agcaaaaaga cttcgctgtt ttctgtcacc accttgacac tggatgattt
     1861 gacacgggca cttgtgagta actgagaacg aatttttgtc gtgagccagg catcacctgc
     1921 cattatgacc ggactgacgg gagtcccctg ccggatttca ttatagacct cctgaacacc
     1981 atcgacaccg agtgctatct gacgggcacg ggcagataat tcacggtccg gagactgacc
     2041 gatcagcagc acgcgtccct gatatgcaat ctcactgatg cgtgcttctt ttctgatttg
     2101 ttcatctttt gaaagtgcac tgctgaccct cagctccagc acttcatcat ccacctgcgt
     2161 tccgacactg cgtgggtcgg ttgctgcctt tgtcccgaca gcggctgtac ccaccacagt
     2221 tgcagcaacg catccctgta acaataaggc tgaaatcagg acaacagcag aatataataa
     2281 acgcatatct cccccggaat ttatcgtaca aaaagtttac cttcagtgaa acaaaacaag
     2341 ctacggactg ttattctctt ccggcacagc cggaaacagc ggatacgtta cgtgacatat
     2401 ctgtatctgc cagacggaaa tgtttatgac ggatatggat gtactgaaat ttaatattgt
     2461 tcatgttttt gcacgaaaac gttgatagcg tagtaaagat tctgtttttt aactacctgg
     2521 tttgctgagc aggatctcct tttcgttata cacaggctaa aaggcaccgt ggccccccgg
     2581 tgcctttttc ataaaacaga gaaaacacgg ctttgaatat acc
//
LOCUS       DAZFLD010000360         2621 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.362, whole genome shotgun sequence.
ACCESSION   DAZFLD010000360 DAZFLD010000000
VERSION     DAZFLD010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2621
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.362"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..2561
                     /locus_tag="ACHKBL_004790"
     CDS             <1..2561
                     /locus_tag="ACHKBL_004790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000842442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LPD7 domain-containing protein"
                     /protein_id="HFO5821525.1"
                     /translation="GDRNVLAQEPNADATVTTTAHDTGTAETDSPSEPAEIQPVQQAS
                     PEPSAKDKPQENVVSATDNDAENTSSATTTLRSNDTPETSVSDATGTQHTATTQPEEP
                     QQVSMGSADDWSAFAEDLNADAPGNATVHSTDNAETKTLSEPAGMQPVQQATPEPTEE
                     NKPQEEGVMSATDTGEESAAAAIDDDLNTVAEDLNADVTVMTTALHTDTAETASPSEP
                     AEIQPVQQATPEPTAEDKPKEERPVSATETSTENEAARPETPSLFSRLRRFLTGGKSR
                     TAEPPVIQENKLTEAETATEAAAPPSPPETSPEDTSSAEPDTIIFAPRRPDSPQRHNL
                     DEILKNLTHKEFPDRTALYSVDGEPAFLDRLYCLEMVDGASADDKKVLAALAVATNFY
                     GGVIELTGSDAFKQKAMQLIVEYDIKVRMKLPAQRAALEKLRQEKEMAHDAIVTHRPT
                     PELNRQSPEETTMAQPAPPVSPKAEAEQTAGSTAETPHPEAPVTAKAEAPRADRPEQP
                     AAPSSAPEKAPVSAQPQQPDTPEEEPPGKLRPGESVTAVLTNYGEKEYAPGKGRCFFV
                     ELTNRSGKREYWGNGLEELVKNHQKGDPVTLTLQARETFRLSGETRDRIRNIWAITPV
                     SNGITVNNDQPHAGQHILEFPMKTFSQVMSQMQQHWPQIMQEVRSPKNPPNNLYIGED
                     RQPASAPQDQSMRLPLAGNAPNELIPAIASADKDNRQLNLLLVHSADDHLQGVVRLNG
                     TLYPALATPSADNRQLVINALTDNGLQFAGYGEAVNHDENTHQRPSPQIMQFHLKQQD
                     SPLFAAIHKPEEQPDKLFRSLGFEQTWKEWSDSQKAEDRQEKTLQQAQSHSPGL"
BASE COUNT          739 a          803 c          645 g          434 t
ORIGIN      
        1 ctggcgaccg aaatgtatta gcacaagaac ccaatgctga cgccacggtt acgaccacgg
       61 ctcacgacac cggcacagca gaaacagatt caccttctga acctgcagag attcagccag
      121 tgcagcaggc cagtcctgaa ccatcggcta aagataaacc acaggagaac gtagtgtctg
      181 ctactgataa cgacgcagag aacacctctt ctgccaccac taccctccgg agtaacgaca
      241 caccggaaac atccgtctcc gacgcaaccg ggacacagca caccgccaca acgcaacctg
      301 aagaaccaca gcaggtctcc atgggcagcg ctgacgactg gagcgcattt gcggaagacc
      361 tcaatgctga tgctccgggg aatgccactg ttcacagcac cgacaacgcg gagacaaaga
      421 cactttctga acctgccggg atgcagcctg ttcaacaggc aacccctgaa ccaacggaag
      481 aaaataaacc tcaggaagag ggcgttatgt ctgctactga tacaggtgaa gagagtgccg
      541 ctgccgctat cgatgacgac ctgaacacag ttgcggaaga cctcaatgct gatgttactg
      601 tgatgaccac agctctccac accgatacag cagaaacagc ctcaccttct gaacctgcag
      661 aaattcagcc cgtacagcag gcaacccctg aaccaacggc agaagataaa ccgaaggaag
      721 agcgtcctgt atctgccact gagaccagca cagagaatga agcagcccgc cctgaaacac
      781 catcgctttt cagccgtctg cgccgttttc tgaccggtgg aaaatcacgt accgccgaac
      841 ctcccgttat tcaggagaac aagcttacgg aggccgaaac tgcgacagag gccgcagcac
      901 caccgtcacc tcctgaaacc agcccggaag acacatcgtc agcggaaccg gacacgatta
      961 tctttgcacc ccgccgtccc gacagtccgc agcggcacaa cctggacgag atccttaaaa
     1021 atcttacaca caaagagttt cccgacagga ctgcgctcta cagcgttgac ggtgaacccg
     1081 ccttcctgga cagactctac tgtctcgaaa tggtggatgg cgccagcgcg gatgacaaaa
     1141 aggtcctggc tgcccttgct gtggccacca acttctatgg cggcgttatc gagctgaccg
     1201 gcagtgatgc ctttaaacaa aaagccatgc agctgattgt cgaatatgac atcaaggttc
     1261 gcatgaaact cccggcccag cgggcagcac tggaaaaact ccgccaggaa aaagagatgg
     1321 cgcatgatgc cattgtcacc cacagaccca cgccggaact caaccgccag tcaccggaag
     1381 agacaacaat ggcccaaccg gctccccctg tgtcaccgaa ggcagaggct gaacaaacag
     1441 caggcagtac ggctgaaaca ccgcatccgg aagcgccggt cacggcaaaa gctgaagccc
     1501 cccgggccga cagaccggaa caacccgccg ccccgtcgtc tgcgccagaa aaagcccctg
     1561 tcagtgcaca gccgcagcaa cccgacacac cggaggaaga gccgcccggc aagctcagac
     1621 ccggtgaaag tgtgactgcc gtcctgacaa actacggaga aaaagagtat gcgcccggaa
     1681 aagggcgctg cttttttgtg gagctcacca accgcagtgg caaacgggaa tactggggaa
     1741 acggcctgga agaactggtc aaaaaccatc agaagggtga ccccgtcaca ctgacactgc
     1801 aggcgagaga aacattccgg ctgtccggtg aaacgcggga tcgtatccgt aatatctggg
     1861 ccataacccc ggtcagtaac ggcattaccg ttaataatga ccaacctcat gcgggtcagc
     1921 atatcctgga atttccgatg aaaacattca gtcaggtaat gtcacaaatg caacaacact
     1981 ggccacagat aatgcaggaa gttcgttccc ccaaaaatcc tcccaataat ctgtacattg
     2041 gtgaagacag gcaacctgca tcagcacctc aggatcaatc aatgcgactc cccctcgctg
     2101 gtaatgcccc caatgaactg ataccagcca tcgcctctgc cgataaggac aatcgtcagt
     2161 tgaatcttct gctggtccac agcgcagatg atcatctgca gggcgtggtc cgcctgaacg
     2221 gcacccttta cccggcactg gccaccccgt cagctgacaa ccgtcagctg gtcatcaatg
     2281 ccctgacgga taacggtctg cagtttgccg gttacggtga agctgttaac catgatgaga
     2341 atactcatca acgtccgtcc ccacaaataa tgcagtttca cctgaagcaa caggactccc
     2401 cgttgtttgc cgccatccac aaaccggaag agcaaccgga caagcttttc cgctctctgg
     2461 gttttgagca gacctggaaa gagtggtctg acagccaaaa ggcggaggac aggcaggaaa
     2521 aaactctgca acaggcacaa tcccacagcc ccggcctgtg agccggtaag gacgaccatg
     2581 aacatgaaaa aaacgctgct ggtcatactg accagcatct c
//
LOCUS       DAZFLD010000361         2616 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.363, whole genome shotgun sequence.
ACCESSION   DAZFLD010000361 DAZFLD010000000
VERSION     DAZFLD010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2616
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.363"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>2616
                     /locus_tag="ACHKBL_004791"
     CDS             <1..>2616
                     /locus_tag="ACHKBL_004791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPD1 domain-containing protein"
                     /protein_id="HFO5821526.1"
                     /translation="ISREEQRNATNRDRTGPERRKGDVSPEQFSDAFGFRGVQFGNYV
                     EGPRRQADLNRAYDSLHDLAEVLNVPTKALSLNGRLGLAFGARGKGKAAAHYEPGEVA
                     INLTKGNGPGALAHEWFHSLDNYFGRYDVSNDGKITSGGDFMTEAQRVRRIFKDGRYV
                     DAEYPVRQEVYDAFKGVIQAIKNSDMPRRSALLDEVRSKPYWSTDVEMAARAFERYVQ
                     DKARMAGVENDYLVNIRKAPEHNTDNTWAYPTNAELDGGIRDAFDHLFSTLKTRETDK
                     GVAFYSRKGVTRTPEGNLISDVNRSAAEAKGSPVPQVEAVARGVMSGIKDSDLKVRVV
                     KSQKEAEALAGESFDGYGRVHAFYRPDKREIVLVADNIPDGRTVREKIRHEIIHHAME
                     HVVTPAEYQTIIKTVLKTRDSDNATIREAWRKVDASYGKESPEVQAGEFLAHMAEKQP
                     NKFVAAWERVVALVKGVLRRTGLLKPTELNDIRLVRETIRTLGQRVREGYTPREDGAG
                     ASSQYSRSGKPDPFKADAYDAKQFASEVRRIAGLQSVPRGGEVRVGDTPPVLRALGAP
                     NKELVMPAHVVHKATRPELREHYVSVETMADLPQLLADPVAVMTSKTEHDSLVTLIEA
                     KDRRGAPVIVAIHMRAKGAVAEVNKIASVYGKDNRIALQEQLDTSLLYLNKEKAADWL
                     LSAGLQLPGANTINGSSGFKVLHPEDIRKGAYYSRTSSLTPEETIASRFVRQMQDKFQ
                     VLKAVQENIRKTGGKIDDSNNAYMAEELFHGKAENDLNVMKERYVQPLAKLLANYKIS
                     QADLDEYLYARHAPERNAHIAKINPKMPDGGSGMTNAEAAEIMQRVRNSGKQAQYDRL
                     AGIVDDMLARRRELIR"
BASE COUNT          650 a          609 c          783 g          574 t
ORIGIN      
        1 atttcgcgtg aggagcagcg taacgccacc aaccgcgacc gtaccggacc agaacgccgt
       61 aaaggggatg tttcaccgga gcagttcagt gatgcgtttg gtttccgtgg tgtgcagttt
      121 ggtaactacg tggaaggtcc gcgtcgtcag gctgatttga accgggctta tgactcgctg
      181 catgaccttg cggaagtact gaatgtaccg acaaaagcgc tttccctgaa cggtcgtctt
      241 ggcctggcat ttggtgcccg tggtaagggt aaggcggcgg cacactatga gccgggtgag
      301 gtggcaatca acctgacaaa aggtaacgga ccgggtgcgc tggcgcacga atggttccat
      361 tctctggata attattttgg tcgttatgac gtttccaatg acgggaaaat tacgtcaggt
      421 ggcgacttta tgacggaagc acagcgtgtc aggcgcatat ttaaagacgg caggtatgtt
      481 gatgctgaat atccggtacg tcaggaggtt tacgacgctt ttaaaggtgt gattcaggcc
      541 attaaaaaca gtgacatgcc gcgtcgttca gcgcttcttg atgaggtgcg ctcaaaaccg
      601 tactggtcaa cggatgttga aatggcggcg cgtgcctttg agcgttatgt tcaggataag
      661 gcgcgtatgg ctggcgtgga gaatgattat ctggtcaata tccgtaaggc acctgagcac
      721 aacacagata acacctgggc ttatccgacg aatgcggaac tggatggcgg tattcgtgat
      781 gcattcgatc acctgttcag tacgctgaaa acccgtgaga cggacaaggg cgttgcgttt
      841 tattcccgta agggcgtaac ccgcacacct gaaggcaatc tcatttcgga tgttaaccgt
      901 agtgcggcgg aagccaaagg cagcccggtc ccgcaggttg aagcggttgc ccgtggcgtg
      961 atgagcggca ttaaggacag tgacctgaag gtccgtgtgg tgaagtcaca gaaagaggct
     1021 gaagcgctgg cgggtgaatc gttcgacggt tacggcaggg tgcacgcgtt ctatcgtccg
     1081 gataaacgtg aaattgtcct ggtggcggat aacatccctg acgggcggac cgtacgcgag
     1141 aagatacgtc acgagataat tcaccatgcc atggagcatg ttgtcacgcc agcggaatat
     1201 cagacgatta tcaaaaccgt gctgaaaacc cgcgacagtg ataacgccac catccgtgaa
     1261 gcctggcgta aggttgatgc gtcctatggt aaggaatcac cggaagtaca ggcgggtgaa
     1321 tttctggcac atatggcgga gaaacagccg aataaattcg tggcggcatg ggagcgtgtt
     1381 gttgccctgg tcaaaggggt actgcgtcgt acggggttac tgaagccgac ggaactgaac
     1441 gatatcagac ttgttcgcga gaccatccgt acgttaggcc agcgtgtgcg ggaaggttac
     1501 acgccgcgtg aggatggcgc gggcgcatcg tctcagtact cccgtagtgg taagccggat
     1561 ccgtttaagg ctgacgccta tgatgcaaag cagtttgctt cagaagtgcg tcgcattgcc
     1621 ggattacaat cggttccacg aggtggagag gtgcgggttg gtgacactcc gccagtgtta
     1681 agagcacttg gcgcaccaaa taaagagctg gttatgccag ctcatgtggt tcacaaggcc
     1741 accagaccag aattacgtga gcactatgtt tctgttgaaa cgatggctga cttaccgcaa
     1801 ttgcttgccg acccggttgc ggtgatgaca tcaaagacgg aacatgactc actggtaact
     1861 ttgattgagg ctaaggacag aagaggtgca cctgtcattg ttgcaattca catgcgtgct
     1921 aaaggggcgg tagctgaggt taataagatt gccagtgtct acggaaagga caacaggatc
     1981 gctttgcagg aacagttgga tacttccttg ctgtatctga ataaagaaaa agccgctgac
     2041 tggcttcttt cagccgggct ccaattgccc ggggcgaaca ccatcaacgg ctcttctgga
     2101 tttaaagtac tacatcctga agatattcgc aagggggcgt attactcccg caccagcagt
     2161 ctgacaccgg aagagaccat tgcatcgcgt tttgttcgcc agatgcagga taaattccag
     2221 gtgctgaagg cggtgcagga gaatatccgt aaaactggcg gaaaaataga tgacagcaat
     2281 aacgcctata tggctgaaga actcttccac gggaaggcgg aaaacgacct gaacgtaatg
     2341 aaggagcgct acgttcagcc gctggccaaa ttgctggcga actacaaaat ctctcaggcc
     2401 gatctggatg agtacctcta cgcccgtcac gcgccggaac gtaacgcaca tattgcgaaa
     2461 atcaacccta aaatgccgga cggcggttcg gggatgacca acgcggaggc ggcggaaatc
     2521 atgcagcgtg tgcgtaacag cggcaaacag gcacagtatg accgtctggc agggattgtt
     2581 gacgatatgc tggcccgtcg ccgtgagctt atccgt
//
LOCUS       DAZFLD010000362         2612 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.364, whole genome shotgun sequence.
ACCESSION   DAZFLD010000362 DAZFLD010000000
VERSION     DAZFLD010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2612
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.364"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..1253)
                     /locus_tag="ACHKBL_004792"
     CDS             complement(<1..1253)
                     /locus_tag="ACHKBL_004792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001289278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbC"
                     /protein_id="HFO5821527.1"
                     /translation="MSQHHVNPDLIHRTAWGNPLWNALHNLNITGLCLAGSIITALIW
                     PLALPVCLLFTLVTSVIFMLQRWRCPLRMPMTLSLDDPSQDRKVRRSLFSFWPTLFQY
                     EADETFPARGIFYVGYRRINDIGRELWLSMDDLTRHVMFFATTGGGKTETTFAWLLNP
                     LCWGRGFTFVDGKAQNDTTRTIWYLSRRFGREDDIEVINFMNGGKSRSEVIQSGDKSR
                     PQSNTWNPFAFSTEAFTAETMQSMLPQNVQGGEWQSRAIAMNKALVFGTKFWCVREGK
                     TMSLQMLREHMTLEGMAKLYCRGLDDQWPEEAIAPLRNYLQDVPGFDMSLVRTPSAWT
                     EEPRKQHAYLSGQFSETFTTFAETFGDVFAADAGDIDIRDSIHSDRILIVMIPALDTS
                     AHTTSALGRMFVTQQSMLLARDLGYR"
     gene            complement(1234..2358)
                     /locus_tag="ACHKBL_004793"
     CDS             complement(1234..2358)
                     /locus_tag="ACHKBL_004793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004031782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbC family protein"
                     /protein_id="HFO5821528.1"
                     /translation="MTIHYTATLNNKHLSLTALYPDGNREVIAILPVTELGALINRNN
                     RLMHLSFSSPFREITETVLVDTVDAERPGHHIGRMLSAIDACLDAEYAGKGKHPSRKN
                     GFWRTLMCGFIAGIALPATVAGYHWMKAEPPSTLAYPTSQEWRAEPVTDTETPRPHTE
                     HHVNSIPARPAPTQQEPSSAPAPTAVTEEQHVQAAEARRNLATVLKRNADRGMFTINL
                     SPGHERTLYAFLDPACINCRLLEPALKQLAREFSVVIYPVSVIGGESSTKRTAPLLCE
                     PDSQKRATGWHQLYSADSGMAPTGDAPAAQDGQCLTAARAAIDVNNQAFRQFGFGGTP
                     WVLSDTGWHLPSGILKDATSVRLFLKATDSEEYHNEPAPR"
     gene            complement(2355..>2612)
                     /locus_tag="ACHKBL_004794"
     CDS             complement(2355..>2612)
                     /locus_tag="ACHKBL_004794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001402878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbA"
                     /protein_id="HFO5821529.1"
                     /translation="SRLGLPCPEPCVEEAIEGLRRDMLGLGLIWDEPQPDRDRKRQIR
                     TRWSLTDDVIPRRHDNDEDTDTGETTETTEKQHPADKEKEQ"
BASE COUNT          528 a          676 c          797 g          611 t
ORIGIN      
        1 cggtagccca ggtcacgggc gaggagcatg ctctgctggg tgacgaacat acgaccgagt
       61 gccgacgtgg tatgagccga tgtgtccagt gcagggatca tcacaatcag aatgcggtcg
      121 ctgtgaatac tgtcgcgaat atcgatgtcg ccggcatctg ccgcaaagac gtccccgaag
      181 gtttcggcaa aggttgtgaa cgtttccgag aactgaccgg agagataagc atgctgttta
      241 cggggctcct cagtccaggc tgacggcgtt ctcaccagtg acatatcgaa tcccgggaca
      301 tcctgcaggt agtttcgcag tggtgcgatg gcttcttccg gccactggtc atcaaggcca
      361 cggcaataga gttttgccat cccttccagc gtcatatgtt cccgtaacat ctgcagggac
      421 atggttttac cttcccggac gcaccagaat tttgtgccaa agaccagtgc cttgttcatg
      481 gctatcgccc gtgactgcca ttcaccgccc tgaacgttct ggggcagcat ggactgcatg
      541 gtttcggcgg tgaaggcttc ggtgctgaat gcgaacgggt tccaggtgtt tgactgtggg
      601 cgggatttat cgccactctg aatgacttca ctgcgggatt ttccgccgtt catgaagttg
      661 atgacttcga tatcatcctc cctgccaaaa cgtcgggaga gataccagat tgtcctcgtg
      721 gtatcgttct gtgccttgcc gtcaacaaag gtaaagcctc ttccccagca gagcgggttg
      781 agcagccagg caaaggtggt ttctgtttta ccgccaccgg tggtggcaaa aaacatgaca
      841 tgccgggtga ggtcatccat gctcagccag agctcacgac cgatatcgtt gatacggcgg
      901 taaccgacat aaaaaatccc ccgggcgggg aatgtttcat ccgcttcata ctgaaacagg
      961 gtgggccaga agctgaagag gcttctcctg acctttctgt cctgggaggg gtcatcaagg
     1021 ctgagtgtca tgggcatacg cagggggcat cgccagcgtt gcagcatgaa tatcacggag
     1081 gtgaccaggg tgaacagcag gcagacagga agggccagtg gccatatcag cgccgtaatg
     1141 atactgccgg ccagacacag accagtgatg ttcaggttat gcagtgcatt ccacaacggg
     1201 tttccccagg ccgtgcggtg tatgaggtca ggattaacgt ggtgctggct cattgtgata
     1261 ctcctcgcta tctgttgctt tcaggaagag gcgaacggaa gtggcgtctt tcaggatgcc
     1321 ggacggcagg tgccagccgg tatcggataa cacccagggg gttccgccaa aaccgaactg
     1381 gcggaaagcc tggttattga catcgatggc agcacgtgcg gcggtcagac actgtccatc
     1441 ctgtgcggcc ggtgcatcgc cggtgggggc cataccactg tcagcggaat acagctgatg
     1501 ccatccggtc gcccgttttt gtgagtccgg ttcacacagc agcggagcag tccgctttgt
     1561 actggactcc ccgccaatca cggagaccgg ataaatcacc acgctgaatt cccgggccag
     1621 ttgcttaagc gcgggttcca gcagacggca gttgatgcat gcggggtcaa ggaaggcgta
     1681 cagcgtgcgt tcatgaccag gggacaggtt aatggtgaac atcccccggt ctgcgttgcg
     1741 tttaagtact gtggccagat tacggcgggc ctcagcggcc tgaacgtgtt gttcttccgt
     1801 cactgccgtc ggggcggggg cagaagaagg ttcctgctgt gtgggagccg ggcgggccgg
     1861 gatgctgtta acatgatgtt ccgtatgtgg gcgtggtgtc tctgtgtcgg ttactggttc
     1921 agctctccat tcctgagatg tagggtaagc cagcgtggaa gggggctctg ccttcatcca
     1981 gtggtaacct gcaactgtcg ccggcagagc aattccggcg ataaagccac acatcagtgt
     2041 ccgccagaag ccatttttcc gggagggatg ttttcctttt ccggcatatt cggcatccag
     2101 gcaggcatca atcgctgaga gcattctgcc gatgtgatgt ccgggacgtt ctgcatcaac
     2161 ggtatcaaca agcaccgttt cggttatttc gcgaaaaggg gaactgaagg acaggtgcat
     2221 cagcctgtta ttacggttaa tcagtgcgcc cagttctgtg actggcagga tggcaatcac
     2281 ctcccgatta ccgtcagggt acagggcggt cagggacagg tgtttattat tcagtgttgc
     2341 agtgtagtgg atggtcattg ttctttttcc ttgtcggcgg gatgctgttt ttcggtggtt
     2401 tcggtggttt caccagtgtc agtgtcttcg tcgttatcat ggcggcgggg tatcacgtca
     2461 tccgtgagtg accagcgggt cctgatctga cgtttacgat cgcgatctgg ctgaggttcg
     2521 tcccatatca ggcccagccc cagcatgtcc cgtcgaagcc cttcaatggc ctcttccaca
     2581 cagggttccg ggcaggggag gccgagacgg ct
//
LOCUS       DAZFLD010000363         2600 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.365, whole genome shotgun sequence.
ACCESSION   DAZFLD010000363 DAZFLD010000000
VERSION     DAZFLD010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2600
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.365"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1713
                     /locus_tag="ACHKBL_004795"
     CDS             <1..1713
                     /locus_tag="ACHKBL_004795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000991381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase NikB"
                     /protein_id="HFO5821530.1"
                     /translation="PEAINVDIRPEKVAETESLKQYACRHFAERLPEMARNGELESCL
                     DVHRTLAKAGLWMGIQHGHLVLHDGFDKQQTPVRADSVWPLMTLDYMQDLDGGWQPVP
                     KDIFTQVIPGERFRGRNIGTQAVSDYEWYRMRMGTGPQGAIKRELFSDKESLWGYTTV
                     QCESLIEDMIAGGNFSWQACHEMFARKGLMLQKQHHGLVIVDAFNHELTPVKASSIHP
                     DLTLSRAEPQAGPFEIAGADIFERVKPECRYNPELAASDEVEPGFRRDPVLRRERREA
                     RAAAREDLRARYLAWKEHWRKPDLRYGERLREIHAACRRRKAYIRVQFRDPQLRKLHY
                     HIAEVQRMQALIRLKESVKEERLSLIAEGKWYPLSYRQWVEQQAVQGDRAALSQLRGW
                     DYRDRRKDKRRTTNADRCVILCEPGGTPLYEDTGVLEARLQKDGSVRFRDRRNGELVC
                     VDYGDRVVFYHHQDRNELVDKLNLIAPVLFDREPGMGFEPEGSYQQFNDVFAEMVAWH
                     NAAGITGNGHFVISRPDVDLHRQRSEQYYHEYIRQQKSISGGHGASYAPVQDNEWTPP
                     SPGM"
     gene            1781..2212
                     /locus_tag="ACHKBL_004796"
     CDS             1781..2212
                     /locus_tag="ACHKBL_004796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0019534 - toxin transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity TolA C-terminal
                     domain-containing protein"
                     /protein_id="HFO5821531.1"
                     /translation="MVLRSIWIEEQYMGVKKIVMLAILVSFVAGCAPLHPSNCHKTTA
                     LGSCSSGRWDDQDEWGEQARGIRDAINAKLDDPQKWKRKKCRLHMEFSRDGTALKIST
                     SNGDKAYCEAIKSAAHKAKFPAFNNPEVYRDFQKSGFDMRG"
     gene            complement(2310..2540)
                     /locus_tag="ACHKBL_004797"
     CDS             complement(2310..2540)
                     /locus_tag="ACHKBL_004797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001369273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5821532.1"
                     /translation="MKTNMGSTKNGARVPHVRNRFTSMDIGMNSQILPDMLINFALIN
                     ITDRKNEGTNTIDGNWQADEGRRYRDNVRIYF"
BASE COUNT          692 a          517 c          737 g          654 t
ORIGIN      
        1 ccggaagcca ttaacgtgga tatccggccg gaaaaagtgg cagaaacgga gagtctgaaa
       61 cagtacgcct gtcgtcactt tgctgaacgc ttaccggaaa tggcgagaaa tggtgagctg
      121 gaaagttgcc tggatgtgca cagaactctg gcaaaggctg gattatggat ggggatccag
      181 cacgggcatc tggtactgca tgatggattt gataaacaac agacaccagt acgtgcagac
      241 agtgtgtggc cgttaatgac gctggattat atgcaggatc tggatggtgg atggcagcct
      301 gtaccgaagg atatttttac tcaggttata cccggagaac gattccgggg acgtaatatt
      361 ggtactcagg ctgtcagtga ttacgaatgg tatcgtatgc ggatggggac aggtccacag
      421 ggagcaataa agcgcgaact gttttctgat aaggaaagtc tgtgggggta cacgactgtt
      481 cagtgtgagt cgttgattga agatatgatc gcgggcggga atttcagctg gcaggcgtgc
      541 catgagatgt ttgcgcgtaa agggcttatg ttgcagaaac agcatcatgg tcttgtgatt
      601 gtggatgcgt ttaatcatga gctcacacca gtgaaggcca gcagtatcca cccggatttg
      661 acgttatcca gggctgagcc gcaggcaggg ccatttgaga ttgcaggtgc agatatattt
      721 gaacgggtga agcctgaatg tcgctacaat ccggaacttg ctgccagcga tgaagtggag
      781 cccggcttcc ggcgcgatcc tgtactgcgt cgtgaacgcc gtgaagcccg tgcagctgcg
      841 cgggaagatt tgcgcgcgcg ttatcttgca tggaaggagc actggcgtaa acccgactta
      901 cgttacggag aacggttacg ggaaattcat gctgcctgcc gtcggcgcaa ggcgtatatc
      961 cgtgtgcagt tccgggatcc acaattgcgt aagctgcatt accatattgc agaagtgcag
     1021 cgtatgcagg cgctgatcag gctgaaggag agtgtgaagg aagagcggtt gtcactgatt
     1081 gcggagggta agtggtatcc tttgtcctac cggcagtggg tggagcaaca ggcggtacag
     1141 ggagacagag ctgcattatc acagttacgt ggctgggatt atcgtgatcg tcgcaaagac
     1201 aagcgacgaa caacgaatgc cgaccgctgc gtgattctct gtgaaccggg aggaactcca
     1261 ctttatgaag acactggcgt tctcgaagcc cgtctgcaga aagatggcag tgtgcgtttt
     1321 cgtgatcgga ggaatggcga gttagtctgt gtggattatg gggaccgtgt ggtgttctac
     1381 catcatcagg acagaaatga actggtggat aagctgaatc tgattgctcc tgtattgttt
     1441 gatcgtgagc caggaatggg ttttgaaccg gaaggctcat atcaacagtt taatgatgta
     1501 tttgcggaaa tggtggcctg gcataatgca gctggaataa ccggaaatgg acactttgta
     1561 atcagccgcc cggatgtgga tttacatcgt cagcgtagtg aacagtacta ccacgaatat
     1621 atcaggcaac agaaaagcat atccggaggg catggcgcat cttatgctcc agttcaggat
     1681 aatgagtgga cgccaccatc acctggaatg tagggatgag gagtggatat taaaaatctt
     1741 aacttgtatt agttaagatt ttctttgctc tgacagagct atggtactga gatctatatg
     1801 gattgaggag caatatatgg gggtaaagaa aatcgttatg ctggctattt tagtcagctt
     1861 tgtggcggga tgtgcgccat tacatccttc aaattgccat aaaactacag ccctgggtag
     1921 ttgcagttca ggacgttggg atgatcagga tgaatggggg gagcaagcac ggggaatcag
     1981 ggatgcaatt aatgctaaac ttgatgatcc gcaaaaatgg aaaaggaaaa aatgcaggtt
     2041 gcatatggaa ttctcccggg atggcacggc gttaaaaata tcaaccagta atggcgataa
     2101 agcctattgc gaagcgataa agtcagcagc ccataaagct aaattcccgg ccttcaacaa
     2161 tccggaagtt tatagagatt ttcaaaaatc cggctttgac atgcgcggtt agctccacga
     2221 catatctgta gggctaacag aagactgtca ggttggctga ttactacact ggaacctgag
     2281 gtactttgcc tcaggttatg ttgacatact caaaaataga ttcttacatt atcccggtaa
     2341 cggcgtcctt catcggcctg ccagttacca tcaatggtat tggtaccctc gttctttctg
     2401 tctgtgatgt taataagggc aaaattaatc agcatgtctg gcaggatctg ggaattcata
     2461 ccaatatcca tagaagtgaa tctattacga acgtggggaa ccctggcacc attttttgtg
     2521 ctgcccatat ttgttttcat gtataattca taatcaaagc gttcatcctg atttagctta
     2581 actgattaaa atccgtagat
//
LOCUS       DAZFLD010000364         2592 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.366, whole genome shotgun sequence.
ACCESSION   DAZFLD010000364 DAZFLD010000000
VERSION     DAZFLD010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2592
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.366"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..965)
                     /gene="pilV"
                     /locus_tag="ACHKBL_004798"
     CDS             complement(<1..965)
                     /gene="pilV"
                     /locus_tag="ACHKBL_004798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001168069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shufflon system plasmid conjugative transfer
                     pilus tip adhesin PilV"
                     /protein_id="HFO5821533.1"
                     /translation="MQKDDDKGFVLFEILAGLIVIGIATPMIYSEIENWLNEQLYQSA
                     AYHADAYNTAARNYIADNNARLHSGSLPANFTADDLIRQGYLKQGFNHSPFGQSYITG
                     IRRNQTTGRLEALTCSTGGQTIKEEGLRSVAGQLPGLGGFISKNGTATGAFGAWTDKP
                     GDYGLTCSTGHIAVVMSGDDLQESDRLYRFQVAGRPELNQMHTAINMGGNNINNTGNI
                     NGQSATLKGDITSEDGWLITRNNKGWMNITHGGGFTMTDSQWIRAVNGKGITTTGEIK
                     GGKVSGGTVRSDGRLSTGEYLQLDKTATAGTKCSPDGLVGRTSTGA"
     gene            complement(970..1605)
                     /locus_tag="ACHKBL_004799"
     CDS             complement(970..1605)
                     /locus_tag="ACHKBL_004799"
                     /EC_number="3.4.23.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HFO5821534.1"
                     /translation="MFLIILLVISATLTIQLLAIRLAGHYLMQVHTLRRPRHLSLLCA
                     VLALTATITHIIRGTDITGLCFLLLMAGCMTLMTLTDAASCRLPRPFTLTFLMLGVAF
                     RLWQGDAAIALACALFWFLLLSLLRRYTSYRKGVETLGLGDVFLIAGVAMWSSANSTP
                     WIIGIAAASTMICMSLFPRSNTGAAIPFGPFICASLYIFTFFPGAIFHTGG"
     gene            complement(1624..2097)
                     /locus_tag="ACHKBL_004800"
     CDS             complement(1624..2097)
                     /locus_tag="ACHKBL_004800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000870986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HFO5821535.1"
                     /translation="MKWFYLSSLFLFCLPATAAVNSNTLDSCFIQAGKRYSIDPGLLK
                     VIARKESSLNPRAINHNRNASGKIISTDYGLMQINSTHIPGLKRMGIIRSENELLSNV
                     CLNIQTGAWILARHFQQCGVNWECLGSYNAGFNKNNTHRRMKYARLIYAAYMQGR"
     gene            complement(2151..2591)
                     /locus_tag="ACHKBL_004801"
     CDS             complement(2151..2591)
                     /locus_tag="ACHKBL_004801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001510726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4 pilus major pilin"
                     /protein_id="HFO5821536.1"
                     /translation="MIIAIVVALAMSQMSSTMASSDNQRELKNLQTIATKMKQYKFQG
                     RYTGTDYIKTLTESGALPADMIAGGNKAKNAWGGDVTIAATADKYGYIITSNNVPKTN
                     CVELINSLRSSSIFTKIMNTAPNTVDPVTVCSSDKNNIKLETNS"
BASE COUNT          611 a          636 c          656 g          689 t
ORIGIN      
        1 gcccctgtac tggtgcggcc tacaagtccg tccggtgaac actttgtgcc tgccgtagct
       61 gttttatcaa gctgtaaata ctccccggtt gaaagacgac cgtcagaccg gactgtacca
      121 ccactgactt tccccccttt gatttccccg gtggttgtga tgcctttgcc attaaccgcg
      181 cgtatccact ggctgtccgt catggtgaat ccaccaccat gtgtgatatt catccatccc
      241 ttattgtttc tggtgattag ccagccgtct tcactcgtaa tatcaccttt cagtgtggca
      301 ctctgaccat tgatattgcc ggtgttgttt atgttattcc cgcccatatt gattgcggta
      361 tgcatctgat tcagttcagg acgacctgct acctgaaaac ggtagagacg gtcactttcc
      421 tgcaggtcat caccgctcat gacgacggcg atgtgacctg tcgaacaggt aagtccgtag
      481 tctcccggtt tatctgtcca ggctccgaat gcgcctgttg ccgttccatt tttgctgata
      541 aatccaccca gccccggcaa ctgacccgcc acactacgca gcccttcttc tttgattgtc
      601 tgccctccag ttgagcaggt cagggcttcc agacgcccgg tggtctggtt acggcggata
      661 ccggtgatat aactctgacc aaagggagaa tggttaaacc cctgtttcag atatccctga
      721 cgaatcaggt catcagccgt gaaatttgcc ggaaggctgc cgctatgcag acgggcattg
      781 ttgtcggcaa tatagtttct ggctgcggta ttgtaggcat ctgcatgata tgcagcactc
      841 tggtaaagct gctcattcag ccagttttca atttctgaat aaatcattgg tgtggcgata
      901 ccgatcacaa tcagtccggc cagaatttca aacagaacaa aacctttatc gtcgtctttt
      961 tgcatggtgt taccctccgg tgtgaaaaat ggcaccagga aaaaaagtga aaatgtagag
     1021 tgatgcacag ataaacggac cgaacggaat agcggcccca gtattactgc gcgggaacag
     1081 cgacatacag atcattgtgc tggctgcggc aataccgatg atccacggag tgctgttggc
     1141 agatgaccac atggccacac ctgcaatcag aaacacgtcc cccagtccca gcgtctccac
     1201 tcccttgcgg tatgaggtgt agcgcctcag gagagataac aggagaaacc agaacagagc
     1261 acaggcaagc gcaatagcag catcgccctg ccataaacga aacgccacac ccagcatcag
     1321 gaatgtgagg gtgaagggac ggggaagacg gcaggaggct gcatcagtca gtgtcatcag
     1381 tgtcatgcag ccggccatca gcaacagaaa gcaaagtccg gtaatgtctg tcccccgtat
     1441 gatgtgcgta atggtggcgg tgagtgccag aacagcacag agcagggaga ggtgacgtgg
     1501 acgccggagg gtatgcacct gcatgagata atgtccggcc agtctgatgg caagtaactg
     1561 tatggtcagc gtagcggaga tcacgagtaa gatgatgagg aacatggctc gtattctccg
     1621 ttattaacgt ccctgcatat atgcggcata aatcagccgg gcatatttca ttctgcggtg
     1681 agtgttattt ttattaaatc cggcgttgta gctaccgaga cactcccagt tcacaccaca
     1741 ctgctgaaaa tgccgggcaa ggatccatgc acctgtctgt atattcaggc aaacattact
     1801 gagcagttca ttttcacttc ggataattcc cattcttttc agtccgggaa tatgtgtgga
     1861 attaatctgc atcaggccat aatctgtgct gataattttt ccgctggcat ttctgttatg
     1921 attgatggcc cggggattca ggctgctttc tttacgggca attactttca gtagtccggg
     1981 atcgatggag taacgttttc ctgcctgaat aaagcagcta tccagtgtgt tactgtttac
     2041 ggcggcagtt gccgggagac agaataagaa aagagatgag agataaaacc atttcatgat
     2101 tatgtgctcc gggaaacagc accccggagg gagtgctgtg tggcagggca ttagctgttg
     2161 gtttccagct tgatattgtt tttatcgcta ctgcagacgg taacgggatc caccgtgttc
     2221 ggggctgtgt tcataatttt ggtaaatatg gacgaactgc gcaggctgtt aatcagctca
     2281 acacagtttg ttttaggaac attattactg gtgataatgt agccatattt gtctgctgtg
     2341 gcagcaatcg tgacatcacc accccatgcg tttttggctt tgttgcctcc tgcaatcata
     2401 tcagcaggca atgcaccgct ttccgtcagg gttttgatat aatccgttcc ggtatatctt
     2461 ccctggaact tgtattgctt cattttggtt gcaattgtct gcaggttttt cagttcgcgc
     2521 tggttgtcac tggaggccat tgtgctggac atctggctca tcgcgagcgc gacaactatt
     2581 gcgatgataa tc
//
LOCUS       DAZFLD010000365         2548 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.367, whole genome shotgun sequence.
ACCESSION   DAZFLD010000365 DAZFLD010000000
VERSION     DAZFLD010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2548
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.367"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(96..314)
                     /gene="ymgF"
                     /locus_tag="ACHKBL_004802"
     CDS             complement(96..314)
                     /gene="ymgF"
                     /locus_tag="ACHKBL_004802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001065870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division-associated protein YmgF"
                     /protein_id="HFO5821537.1"
                     /translation="MNNSNNLDYFTLYIIFSIAFMLITLLVILIAKPSTGLGEVLVTI
                     NLLNALVWLAINLINRLRERLVSHRDQH"
     gene            complement(446..1969)
                     /locus_tag="ACHKBL_004803"
     CDS             complement(446..1969)
                     /locus_tag="ACHKBL_004803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFO5821538.1"
                     /translation="MRNTLIPILVAICLFITGVAILNIQLWYSAKAEYLAGARYAANN
                     INHILEEASQATQTAVNIAGKECDLEEQYQLGTEAALKPHLRTIIILKQGTVWCTSLP
                     GNRVLLSRIPVFPDSNLLLAPAIDTVNRLPILLYQSQFADTRILVTISDQHIREALNV
                     PLKGVRYVLRVADDIIGPTGDVMTLNGHYPYTEKVHSTKYHFTIIFNPPPLFSFYRLI
                     DKGFGILIFILLIACAAAFLLDRYFNKSATPEEILRRAINNGEIVPFYQPVVNGREGT
                     LRGVEVLARWKQPHGGYISPAAFIPLAEKSGLIVPLTRSLMNQVARQMNAIASKLPEG
                     FHIGINFSASHIISPTFVDECLNYRDSFTRRDLNLVLEVTEREPLNVDESLVQRLNIL
                     HENGFVIALDDFGTGYSGLSYLHDLHIDYIKIDHSFVGRVNADPESTRILDCVLDLAR
                     KLSISIVAEGVETKEQLDYLNQNNITFQQGYYFYKPVTYIDLVKIILSKPKVKVVVE"
     gene            complement(2305..>2548)
                     /gene="ymgC"
                     /locus_tag="ACHKBL_004804"
     CDS             complement(2305..>2548)
                     /gene="ymgC"
                     /locus_tag="ACHKBL_004804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protein YmgC"
                     /protein_id="HFO5821539.1"
                     /translation="NSIPERFILQCALFKNLEREVFMTHGYVDSYIIDQALRLRLKDE
                     TSVILSDLYLQILQYIEMHKTTLTDIIINDRESVIS"
BASE COUNT          771 a          569 c          527 g          681 t
ORIGIN      
        1 acaactattt gcgcaataat tcctttcttc aatgatccag gcgttcactg ccagcaaccc
       61 attcagccgc atactgacag tgagaaactg aaagattagt gctgatccct gtggcttacg
      121 agtctttccc ttaatcgatt aatcagattg atcgccagcc aaacaagggc attaagcaaa
      181 tttatcgtca caagcacttc tcccagcccg gtactgggtt ttgcaataag gatgaccagg
      241 agagtgatca gcataaatgc aatggaaaag atgatataga gagtgaaata atccagatta
      301 ttactattgt tcatgctact aatggcaatc ctgtcaaaac tttcaaacaa ttatagcaaa
      361 caaaataatg aacaatagta acaagaggca acgagctaat tagctgtcta taattacacc
      421 gcactatggc cttactgatc gattttcact caaccacaac cttcaccttc ggtttagaaa
      481 ggataatttt gaccaggtcg atgtatgtaa caggtttata gaaataataa ccctgctgaa
      541 atgtgatatt attttgattc agatagtcaa gttgttcttt cgtttcgaca ccttcagcga
      601 cgatactgat cgaaagttta cgcgccagat ccaatacaca atccagaatt cgggttgatt
      661 ctgggtctgc gttgacgcgg ccaacgaaac tgtgatcaat tttgatataa tcaatatgca
      721 ggtcatgcag ataagaaagc cctgagtagc cagtaccgaa atcatccagc gcgatgacaa
      781 aaccattttc atgcagaatg ttcaaccgct gaaccagact ttcatcaaca tttaatggct
      841 cacgctcggt aacttccaga acgaggttta aatcgcggcg ggtaaaactg tcacggtaat
      901 ttaagcactc gtcgacaaac gtcggcgaaa taatatgcga ggcgctaaaa ttaattccaa
      961 tatgaaaacc ttccggcaat ttactcgcga tagcgttcat ctgtctggca acctgattca
     1021 tcaggcttcg cgtaagcgga acgattaatc cggatttttc agcaagtgga ataaatgccg
     1081 caggtgatat atatccaccg tgaggttgtt tccagcgggc taacacctca actccccgca
     1141 atgtcccttc ccgaccattt accacaggtt ggtaaaaagg gacgatctcc ccattattta
     1201 tagcccgtcg caggatctct tcaggcgttg cgcttttatt gaaataccta tcaagcagga
     1261 aggcagcggc gcaggcgatt aacagaataa atatcaaaat cccaaagcct ttatcgataa
     1321 gtctgtagaa gctaaagagt ggcggcgggt taaagataat agtgaaatga tattttgtag
     1381 agtgaacctt ctcggtatag ggatagtgtc cattaagcgt catcacatca cccgtaggtc
     1441 caataatgtc atctgccacg cgtaatacat accttacccc tttcaagggt acattaagcg
     1501 cctcgcgaat atgctgatca cttatcgtaa ccaaaatgcg cgtatctgca aattggctct
     1561 gatagagcag gataggtaat ctattaacgg tgtcgattgc cggagccagc agtaaattac
     1621 tgtccgggaa aacaggaata cgagacagta ggacccgatt ccccggtaag gatgtacacc
     1681 agaccgttcc ctgtttgaga atgatgattg tgcgcaggtg aggcttcaga gctgcttcag
     1741 tgccaagctg atattgctcc tcgaggtcgc attccttccc ggcaatgtta accgccgttt
     1801 gagtcgcctg tgacgcttct tcaagtatat gattgatatt gttggcggca tatctcgctc
     1861 ccgccaggta ctctgctttc gcggaatacc agagctggat gttcaaaata gcgacaccgg
     1921 ttataaacaa gcatatcgca acgaggatgg gtatgagtgt attgcgcatt cgcttgtccc
     1981 ggacaaaggg tttaatacat gaaaatgacg ttcattcgct cgcgctggcg tctacagaga
     2041 gcatggtaga gagcatcaca aatatccacg ccgaagataa aattttctcc aacttcatct
     2101 aacctattaa ttattgagca caatgtaggc agaattaaac aaattaggtg ggatatcagg
     2161 cgtcaagaat gaaaaacgag cgctctccat tcctgtttgc cccctgatat tgcggatata
     2221 ttaagaaagc ataacgcctg aaatgctcac tttgcaccag catggtgata cagctgatgt
     2281 ttattctata accttactca agttctaaga gatcacggat tccctgtcat taataatgat
     2341 atccgttagt gtagtcttat gcatttcaat gtactgcaat atttgcagat agagatcaga
     2401 aagaatcaca ctggtttcat cttttaaacg caagcgtaaa gcctgatcaa taatataact
     2461 atcaacatac ccatgcgtca tgaacacctc cctctcgaga tttttaaaca aagcacattg
     2521 caaaataaat ctctctggga ttgaatta
//
LOCUS       DAZFLD010000366         2546 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.368, whole genome shotgun sequence.
ACCESSION   DAZFLD010000366 DAZFLD010000000
VERSION     DAZFLD010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2546
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.368"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(85..939)
                     /gene="rclR"
                     /locus_tag="ACHKBL_004805"
     CDS             complement(85..939)
                     /gene="rclR"
                     /locus_tag="ACHKBL_004805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine-specific transcriptional
                     regulator RclR"
                     /protein_id="HFO5821540.1"
                     /translation="MDALSRLLMLNAPQGTIDKNCVLGSDWQLPHGAGELSVIRWHAL
                     TQGAAKLEMPTGEIFTLRPGNVVLLPQNSAHRLSHVDNESTCIVCGTLRLQHSARYFL
                     TSLPETLFVAPVNHSVEYNWLREAIPFLQQESRSAMPGVDALCSQICATFFTLAVREW
                     IAQVNTEKNILSLLLHPRLGAVIQQMLEMPGHAWTVESLASIAHMSRASFAQLFRDVS
                     GTTPLAVLTKLRLQIAAQMFSREMLPVVVIAESVGYASESSFHKAFVREFGCTPGEYR
                     ERVRQLAP"
     gene            1165..2490
                     /gene="rclA"
                     /locus_tag="ACHKBL_004806"
     CDS             1165..2490
                     /gene="rclA"
                     /locus_tag="ACHKBL_004806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine resistance oxidoreductase
                     RclA"
                     /protein_id="HFO5821541.1"
                     /translation="MNKYQAVIIGFGKAGKTLAVTLAKAGWRVALIEQSNAMYGGTCI
                     NIGCIPTKTLVHDAQQHTDFVRAIQRKNEVVNFLRNKNFHNLADIPNIDVIDGQAEFI
                     NNHSLRVHRPGGNLEIHGEKIFINTGAQAVVPPIPGITTTPGVYDSTGLLNLKELPGH
                     LGILGGGYIGVEFASMFANFGSKVTILEAASLFLPREDRDIADNIATILRDQGVDIIL
                     NAHVERISHHENQVQVHSEHAQLAVDALLIASGRQPATASLHPENAGIAVNERGAIVV
                     DKRLHTTADNIWAMGDVTGGLQFTYISLDDYRIVRDELLGEGKRSTDDRKNVPYSVFM
                     TPPLSRVGMTEEQARESGADIQVVTLPVAAIPRARVMNDTRGVLKAIVDNKTQRILGA
                     SLLCVDSHEMINIVKMVMDAGLPYSILRDQIFTHPSMSESLNDLFSLVK"
BASE COUNT          719 a          573 c          594 g          660 t
ORIGIN      
        1 accaccgttg gaaatagaaa tagttgaaag gctcacaact cccggaatgc ggattatctt
       61 taccctgatt tctggcggtt ttattcaggg tgcaagctgt ctcacccttt cccgatattc
      121 tcccggagta caaccaaact cgcggacaaa cgccttgtga aaagatgatt cactggcata
      181 gcctactgac tcagcgatca ccacaacagg gagcatttcc cgggaaaaca tctgggccgc
      241 tatttgtaga cgcaactttg ttaatacagc cagcggcgtg gttccggaaa catcacggaa
      301 aagctgggca aaacttgccc gggacatgtg ggcgatgctg gccagcgatt cgacggtcca
      361 ggcgtgtcct ggcatttcca gcatttgctg tattaccgca ccaagacgtg gatgcagaag
      421 caaactgaga atgtttttct ctgtattaac ctgtgcaatc cactcacgca ccgcgagggt
      481 aaagaatgta gcgcagatct ggctacacag agcatccact cccggcattg ccgatctgga
      541 ttcctgttgt aaaaacggga tcgcctccct cagccagtta tattccacgc tatggttcac
      601 tggtgctaca aacagcgttt ccggcaaaga tgttaaaaaa taacgcgccg aatgttgcag
      661 ccgaagagtg ccacagacaa tacaggtcga ttcattatcg acatgactca ggcgatgagc
      721 tgaattttgt ggtagcagga ccacatttcc cgggcgtaat gtaaaaatct cccccgtcgg
      781 catttccagc ttcgccgctc cttgcgttaa cgcatgccaa cgaataaccg acaattcccc
      841 ggcaccatgt ggaagttgcc agtcacttcc taacacgcaa ttcttatcga tcgttccttg
      901 tggagcgtta agcatcaaca aacggctaag ggcatccata tcagactcct gagtggtttt
      961 tgattacaaa caccaacaat cttagacaat aattaatcta atatcatcaa atggttaatc
     1021 atgatttttg ttttgtgatt ctgattaaga aaaagaaaat accagacgat aagacaaaat
     1081 atcaagcgtt gtgaagaaat gttatttgct cttttgcgtc tatagtcatg atgtcaaatg
     1141 aacgcgtttc gacaggaaat catcatgaat aaatatcagg cagttattat tggttttggc
     1201 aaggctggaa aaacattagc cgtcacgctg gcaaaagcag gttggcgtgt ggctctcatc
     1261 gaacaatcaa atgcaatgta tggcgggacc tgtattaata tcggctgtat cccaaccaaa
     1321 acattggttc atgacgcaca gcagcacaca gattttgtcc gtgccataca gcgtaaaaat
     1381 gaagtggtta attttttacg taataagaat tttcataatc ttgcggatat acccaatatc
     1441 gacgtgatcg acggccaggc ggagtttatc aataatcata gcctgcgtgt tcatcggcct
     1501 gggggaaatc tggagattca tggcgagaaa atttttatta ataccggtgc acaagccgtg
     1561 gttccgccaa ttcctggaat taccaccacg cctggtgtat atgacagcac cggattactt
     1621 aatctaaaag aattgcctgg gcatttaggt attttgggcg gcggatatat tggcgttgag
     1681 ttcgcctcta tgttcgctaa ttttggcagc aaagtaacca ttttagaggc agcttcgctg
     1741 tttttgcctc gggaagatcg ggatattgct gataatatcg cgacgatttt acgcgatcag
     1801 ggtgtcgata taatcctcaa tgcccatgtg gagcgaatca gtcaccatga aaatcaagtg
     1861 caagtgcata gcgagcacgc ccaactggcg gtggatgcac tgttaatagc ttccggtcgt
     1921 caaccagcta ccgcttcgtt acatccagaa aatgccggta tcgcagtaaa cgagcgcggg
     1981 gcaattgtcg ttgacaagcg attacatacc accgcagaca atatttgggc gatgggagat
     2041 gttaccggcg ggctgcaatt tacttacata tcactagatg attaccgcat tgtacgtgat
     2101 gagttactgg gtgaaggcaa acgtagtact gatgatcgga aaaatgtgcc ttattccgta
     2161 tttatgacac cgcccctgtc cagggttggt atgacagaag aacaagccag agagagtggt
     2221 gctgatattc aggtggtgac attgcctgta gctgcaattc cgcgcgccag agtgatgaac
     2281 gatacccgtg gggtattaaa agcgattgtt gataataaaa cccaacgtat attaggggca
     2341 tcactgctat gtgttgactc ccacgagatg atcaatatag tgaaaatggt gatggatgcc
     2401 gggctgcctt atagcatatt acgcgatcag atatttactc atccgtcgat gagcgaatca
     2461 ctcaatgatc tattttcatt agtcaaataa actcaaaatc agacgccaga acaaatatcc
     2521 tggcgtctca gagaaaagaa tcttat
//
LOCUS       DAZFLD010000367         2522 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.369, whole genome shotgun sequence.
ACCESSION   DAZFLD010000367 DAZFLD010000000
VERSION     DAZFLD010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2522
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.369"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(538..765)
                     /locus_tag="ACHKBL_004807"
     CDS             complement(538..765)
                     /locus_tag="ACHKBL_004807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001371659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5821542.1"
                     /translation="MRKRDDYYDGLIAGGTVAIAVATLLTLLITVFVNNKENSEFHEW
                     INSHCEKENVENTSFKCDDGDGNIYIVKKEA"
     gene            complement(795..1079)
                     /locus_tag="ACHKBL_004808"
     CDS             complement(795..1079)
                     /locus_tag="ACHKBL_004808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001371664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HFO5821543.1"
                     /translation="MALKLNRPNIDESQQPATNAETARFISGATRAPVVGKPKLMNFR
                     LPAYFEDILEAEAQRTGQSKTTVLKAALAAFDNLDENLKNHWLLESAKLG"
     gene            complement(1178..1840)
                     /locus_tag="ACHKBL_004809"
     CDS             complement(1178..1840)
                     /locus_tag="ACHKBL_004809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division plane positioning ATPase MipZ"
                     /protein_id="HFO5821544.1"
                     /translation="MGKILLVVSDKGGVGKSTYVANTGSMLVNKGKSVIILKTDKNHD
                     LLSWNEKRTDNGLLTIPVHAAYGNVSNEIKRLSKLCEVLIVDCPGHDSQEFRSALTVS
                     DIVVTLVKPSSDFESETLTSVTEKIRTAQKANAALEPWVLFTRINSAKPRHRKAAIDL
                     DKLLRSDNIWIQPLKTRISELDVYESACNEGAGVHDVSRASSLSTAKAQIELVAQEIG
                     IL"
     gene            complement(2268..>2522)
                     /locus_tag="ACHKBL_004810"
     CDS             complement(2268..>2522)
                     /locus_tag="ACHKBL_004810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001236005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5821545.1"
                     /translation="TPLTVRRLCKALFGRTGSQWLVVEVFGEKGRQHRSADSNPEMVE
                     KMAARYRHAAELHWSATLAEIERVKRLYQTKIKKSKKEVG"
BASE COUNT          649 a          560 c          505 g          808 t
ORIGIN      
        1 gcgaatatgt agatcttaga tctatattaa tccatttcca cagaatttta cactaaaaac
       61 aaccgcacaa atagatcgta gatctaccga ttgataaaaa aacaaaataa cagatcgtgt
      121 tagtaccaat ttccagataa atattttttt gatgttcttt tttcatactg ttttcgttga
      181 acttttgaac aaccaagcca gcactgcaca cgcagtcgtc atacagggag gagagccgaa
      241 accgtcgaaa cttccgatat agcgaatctg ttgctttttt gaagcctgtt tggtaagtac
      301 tgacatgtac tgtccccagc agcaggtacg accatggagt taacagcgtc ttgatcagtt
      361 aagttaaaat gaaagcgaaa acagttatca gaacgggcgt tgtagttgcg ctaatcggga
      421 ttgcaggcgt attacttgaa aaaatcgtct ggcaaacagc agacacatat ccagcacttc
      481 tggctgattc gtcacgactg tagggcgtta acaggaatgg tccctccccg cccttaatta
      541 cgcttccttt tttacgatat atatattccc atccccgtca tcacacttaa atgaagtgtt
      601 ttcaacattt tctttttcac agtgtgagtt tatccattca tgaaactcgg aattttcctt
      661 attgttgaca aatacagtaa ttaacagtgt cagaagggtt gcaacagcaa ttgccacagt
      721 acctccagca attaacccgt cataataatc atctctcttt ctcatttttt aaacctaaat
      781 aagcgaatta cttgttaacc taactttgca gactcaagta gccaatgatt tttcaggttc
      841 tcgtccaggt tatcgaatgc ggccagcgct gcctttagaa cggttgtctt gctctggcct
      901 gttctctgcg cctccgcttc caggatatcc tcaaagtacg ctggcagtct gaaattcatc
      961 aactttggtt tacccactac aggcgctctg gtagcgccgg agataaaacg cgcggtttct
     1021 gcgtttgttg ctggttgctg agattcatcg atgttaggac ggtttagttt taaagccatg
     1081 aaatttcccc tagtacggta tgtattgata gtaaatatca taccgccttt acattatctt
     1141 tacaatgtaa agataataat ttaacatatt gatattacta caaaatccca atttcctgag
     1201 ccacgagttc aatctgtgct ttcgctgttg aaaggcttga tgcccggcta acatcatgta
     1261 cccctgcccc ttcattacag gcgctttcat acacatccag ctcggatatc cgggttttca
     1321 gaggttgaat ccagatgttg tccgagcgca gtaatttatc cagatcaatg gccgcttttc
     1381 tgtggcgggg tttagctgag ttgattcttg tgaacagcac ccacggttcc agggctgcgt
     1441 tagctttctg agcggtgcgg attttctctg taacactggt cagtgtttcg ctttcgaaat
     1501 cagaggacgg cttaactagt gttaccacga tatctgatac tgtcagggcg ctacggaact
     1561 cctggctgtc gtggccgggg caatcgacaa tgagcacttc acacagctta ctaaggcgct
     1621 taatttcgtt gctgacgttc ccataagctg cgtgaaccgg gatagtgagt aagccgttat
     1681 ctgtccgctt ttcgttccag ctcaacagat cgtggttttt atctgtcttc aagatgatta
     1741 ccgacttacc tttgttgacc agcattgagc ctgtattagc cacgtaggtg cttttgccta
     1801 caccaccctt atctgatacg acaagcaaga ttttacccat ttcagaattc ctctctttat
     1861 gatgtaaaga atgtgtaaag actctattgt ctttacaatg tgtgattttt gtaaagatga
     1921 ttttgtcttt acagtgtctt tatattacat aagctttacc atgtaaagca caaataaaga
     1981 taccattatc tttataatac ctttactttg taaaggtatt tgcgtcttta tttttgcgtt
     2041 acgtggtaaa gctgcatttg tctttacatt atctttatgt tgtaaaacat gtgtatgaca
     2101 ttgtcacttt ctggttatgg gtacgttttc tggttctttt tgagtgtgag tgagtttgag
     2161 gggtcagttt ggatattcga aattattgta cgttcaacgc accatcatta atgctaatgt
     2221 atgcatacat tagcattaat gatgaaggaa aagaataaca atggcagtca acccacttct
     2281 tttttggatt tcttaatttt cgtctgataa agccgcttta cccgttcaat ttctgccagc
     2341 gttgcggacc agtgtagttc ggcggcatga cgatatctgg cagccatttt ttccaccatc
     2401 tccggattgc tgtccgcgct gcggtgctga cgccccttct ccccaaaaac ttcaacaacc
     2461 agccactggc ttccggttct gccgaacaat gccttgcaca aacgtctcac agtcagcggg
     2521 gt
//
LOCUS       DAZFLD010000368         2499 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.370, whole genome shotgun sequence.
ACCESSION   DAZFLD010000368 DAZFLD010000000
VERSION     DAZFLD010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2499
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.370"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..1333)
                     /gene="emrY"
                     /locus_tag="ACHKBL_004811"
     CDS             complement(<1..1333)
                     /gene="emrY"
                     /locus_tag="ACHKBL_004811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
                     /protein_id="HFO5821546.1"
                     /translation="MAITKSTPAPLTGGTLWCVTIALSLATFMQMLDSTISNVAIPTI
                     SGFLGASTDEGTWVITSFGVANAIAIPVTGRLAQRIGELRLFLLSVTFFSLSSLMCSL
                     STNLDVLIFFRVVQGLMAGPLIPLSQSLLLRNYPPEKRTFALALWSMTVIIAPICGPI
                     LGGYICDNFSWGWIFLINVPMGIIVLTLCLTLLKGRETETSPVKMNLPGLTLLVLGVG
                     GLQIMLDKGRDLDWFNSSTIIILTVVSVISLISLVIWESTSENPILDLSLFKSRNFTI
                     GIVSITCAYLFYSGAIVLMPQLLQETMGYNAIWAGLAYAPIGIMPLLISPLIGRYGNK
                     IDMRLLVTFSFLMYAVCYYWRSVTFMPTIDFTGIILPQFFQGFAVACFFLPLTTISFS
                     GLPDNKFANASSMSNFFRTLSGSVGTSLTMTLWGRRESLHHSQLTATIDQFN"
     gene            complement(1333..2496)
                     /gene="emrK"
                     /locus_tag="ACHKBL_004812"
     CDS             complement(1333..2496)
                     /gene="emrK"
                     /locus_tag="ACHKBL_004812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrK"
                     /protein_id="HFO5821547.1"
                     /translation="MEQINSNKKHSNRRKYFSLLAVVLFIAFSGAYAYWSMELEDMIS
                     TDDAYVTGNADPISAQVSGSVTVVNHKDTNYVRQGDILVSLDKTDATIALNKAKNNLA
                     NIVRQTNKLYLQDKQYSAEVASARIQYQQSLEDYNRRVPLAKQGVISKETLEHTKDTL
                     ISSKAALNAAIQAYKANKALVMNTPLNRQPQVVEAADATKEAWLALKRTDIKSPVTGY
                     IAQRSVQVGETVSPGQSLMAVVPARQMWVNANFKETQLTDVRIGQSVNIISDLYGENV
                     VFHGRVTGINMGTGNAFSLLPAQNATGNWIKIVQRVPVEVSLDPKELMEHPLRIGLSM
                     TATIDTKDEDIAEMPELASTVTSMPAYTSKALVIDTTPIEKEISNIISHNGQL"
BASE COUNT          747 a          551 c          499 g          702 t
ORIGIN      
        1 ggttaaattg atcgatggtt gctgtcaact gactatggtg taacgattcg cgtcgtcccc
       61 acagcgtcat tgtcaacgac gtaccaactg atcctgacaa ggtacgaaaa aaattactca
      121 tactcgaggc attggcaaat ttattatctg gcaaccctga aaacgaaatc gttgttaagg
      181 gtaaaaagaa acaggcaacg gcgaatccct ggaaaaactg tggcaaaatg atgcctgtaa
      241 aatcaatcgt tggcataaat gtcacagaac gccagtaata gcaaaccgca tacatcaaaa
      301 aactaaatgt caccaacaac cgcatatcaa ttttgttgcc ataacgtcct atcaaaggtg
      361 atattaatag cggcatgatg ccgatgggcg cataggcaag tccggcccat atcgcattat
      421 accccatcgt ttcctggagt aactgcggca taaggactat cgccccagag taaaataaat
      481 acgcgcatgt gatactcaca ataccaatgg tgaaattacg ggacttaaac aaactgagat
      541 caagaatcgg attctctgag gtcgactccc aaatgactaa agaaatcaga gaaataactg
      601 acactactgt tagtattatt attgtactcg agttgaacca atccagatcg cgccctttat
      661 caagcattat ctgcaagcca ccaacaccga gcactaacag ggtcagtcct ggtagattca
      721 ttttgaccgg tgaagtctca gtttctcttc ctttaagtaa ggttaagcat aatgtcagaa
      781 cgataatccc catagggaca ttgattaaaa atatccaacc ccagctaaag ttatcgcaaa
      841 tatagccgcc caatatcggc ccacatatcg gagcgataat tacggtcatt gaccataatg
      901 ccaaagcaaa tgttcttttt tctggcggat aattccttaa taataaactc tgtgacagtg
      961 gaattaacgg ccccgccatt aagccctgaa cgactctaaa aaatatcagc acatcaagat
     1021 tggtcgatag gctacacatt aatgaagata gtgaaaaaaa ggttactgaa agtaaaaata
     1081 atcttaattc gcctattctt tgtgccagcc tgccggtaac agggatcgca atggcatttg
     1141 ctacgccaaa cgaagtgata acccaggtgc cttcgtctgt tgatgctccc agaaagccag
     1201 atattgtcgg tattgcgacg ttagaaatag tggaatccaa catttgcata aacgtcgcta
     1261 aggacaatgc aatagtaacg caccataacg tcccaccggt taatggtgcc ggagttgatt
     1321 tagtgattgc cattaaagtt gtccattatg cgaaataatg ttgctaattt ctttttctat
     1381 cggagtagta tcgataacta aagccttact ggtataagca ggcatggagg tcacggttga
     1441 agccagctca ggcatctcag caatgtcttc gtccttcgta tcaatagttg cagtcatcga
     1501 taaaccaata cgtaagggat gttccatgag ttcttttgga tcaagagaaa cttcaaccgg
     1561 tacacgctga acgattttga tccagttccc tgtcgcattt tgtgcaggta ataaggagaa
     1621 cgcattgccg gttcccatat tgattcctgt cacccgacca tgaaacacaa cattttcacc
     1681 ataaagatcg ctgataatat tgaccgattg accaatccgt acatccgtga gttgtgtttc
     1741 tttaaagttg gcattaaccc acatttgacg tgccggtacg acagccatta acgattgtcc
     1801 gggactcact gtttcgccga cctgaacact tcgctgggca atatagccgg taacaggact
     1861 cttaatatcc gtacgtttaa gcgctaacca ggcttctttg gttgcatctg ccgcttcaac
     1921 gacttgtggc tgacggttta atggtgtgtt cattactaaa gctttattcg ctttataagc
     1981 ctggatagcg gcattcaatg ccgctttgct acttattaac gtatctttgg tatgctccag
     2041 cgtttctttt gaaataaccc cctgcttcgc taaaggcaca cgacggttat aatcttctaa
     2101 agattgttga tactgaatac gtgctgaagc gacttcggca ctgtattgtt tatcctgtaa
     2161 gtacagttta ttcgtttgcc gaacaatatt tgccagatta tttttagctt tattgagtgc
     2221 gatagtggca tcagtcttat ccagtgagac taaaatgtca ccctgtcgaa cgtagttcgt
     2281 atctttatga ttaacgacag tgacgctgcc tgaaacttgt gcagaaattg gatctgcatt
     2341 ccccgtgaca taggcgtcat ctgtactaat catgtcttct aattccattg accaataggc
     2401 ataggcacct gaaaacgcaa taaataaaac taccgccaat aaagaaaagt attttcttct
     2461 gttagaatgt tttttatttg aattaatctg ttccacttt
//
LOCUS       DAZFLD010000369         2496 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.371, whole genome shotgun sequence.
ACCESSION   DAZFLD010000369 DAZFLD010000000
VERSION     DAZFLD010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2496
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.371"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..252
                     /locus_tag="ACHKBL_004813"
     CDS             <1..252
                     /locus_tag="ACHKBL_004813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001604127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5821548.1"
                     /translation="PYSWHWNVRFRPQEAVYIGNDACHITITCNQSGFHLTRDGQRLF
                     TERYIRTLSELLAVLKRRWDVTPAIIRAVEYLSRVPVSH"
     gene            307..1671
                     /locus_tag="ACHKBL_004814"
     CDS             307..1671
                     /locus_tag="ACHKBL_004814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000200223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VasL domain-containing protein"
                     /protein_id="HFO5821549.1"
                     /translation="MTSEVKLKTGGDPRSLPDYAALRDEISKLTHPARPDVDWRYVET
                     LCLRLYEHNGVELQTVSWYTIARMHTAGLSGLNEGLALIVALTRHHWSVMWPLNTHAR
                     LEIITGLFSRLQKTLRAMPPDERDNLPLLYQTETCLKTLSDILARHELKQSSKVALPE
                     AMVKGYITRLENQPVQGEVSSPVTLPAQALRSDAPDVQEHQPLPYSRLVYVVSETKTE
                     PTLFLQKSPLTFLKTFVTGVCTALLAVCVAIPGWQFLTQPSPAEQQLRALLAPLPDVL
                     SADQMAQLSRTPSLLNHTSEWITLSGQQITRLTELPPAYNLQRSAQLLQQLRGLFPDN
                     PRVQEMVDNWHKSVRSRALPEEAMTGWNEGMARLQQLAERLNRLDEQRGKYMTVSELK
                     TEVFGIMQAFNRHIPAEEQLRRYDEARNQNGSEQQQKQAEMALNQLINRYQMENAGKP
                     KSQP"
     gene            1687..2319
                     /locus_tag="ACHKBL_004815"
     CDS             1687..2319
                     /locus_tag="ACHKBL_004815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SANT/Myb-like DNA-binding domain-containing
                     protein"
                     /protein_id="HFO5821550.1"
                     /translation="MEMENTPGRARTPWTIEDVRYLETHYGVKKTREIAAYLNRSATA
                     VRAMAYLVGITTPVAADWSETEIEIIRRDYPAGVALADICARLNGRAPKSVCWMARRF
                     GLKRLGYWSAEEDEILRQYYPSEGVKVSRRLSGRTENAVKLRARELGVKFTGNGRLRL
                     WTAEEWNVLSGNMASGLSALMKLLPGRSRSSVQGAMRRLQKMSAQKSVRQ"
BASE COUNT          666 a          593 c          691 g          546 t
ORIGIN      
        1 ccgtattcct ggcactggaa tgtcagattc aggccacagg aagccgtata tattggtaat
       61 gatgcatgcc atattaccat tacctgtaat cagtccggtt ttcatttaac cagggacggg
      121 cagcgactgt tcactgaacg ttatatcaga acactcagtg agttattagc ggttctgaag
      181 cggcggtggg atgtgacacc ggcaatcatc cgtgcggtgg aatacctttc ccgggtaccg
      241 gtatcacact gatgtccttt ccgtaactgt taatcatgat ttaatacagc aacaccgaat
      301 ctgccgatga cttctgaagt gaaactgaaa accggtggtg acccccggtc attaccggat
      361 tatgctgccc tgcgtgatga aatcagcaaa ctgacccacc cggcacgtcc ggatgtggac
      421 tggcgttacg tggaaacgct ctgcctgcgt ctgtatgaac acaatggcgt ggaattacag
      481 acggtatcct ggtacaccat tgcccggatg catacagcag ggttaagtgg tctgaatgaa
      541 gggctggctc tgattgtggc gctgacccgg catcactggt cagtgatgtg gccgctgaat
      601 acccacgccc ggctggaaat tattaccggt ctgttcagtc gtctgcaaaa aacgctccgg
      661 gccatgccac cggatgagcg tgacaacctg cccctgctgt atcagacaga aacatgcctg
      721 aaaacgctga gtgacatact ggcccggcat gaacttaaac agagcagtaa agtggcttta
      781 ccggaagcca tggtgaaggg atatatcacc cgtctggaga atcagcctgt gcagggtgaa
      841 gtatcgtcgc cggtaaccct tccggctcag gcactccggt ctgatgctcc ggatgtacag
      901 gaacatcagc ccctgccgta ctcccgtctg gtgtatgttg tttctgaaac aaaaacagag
      961 cccacactct tccttcaaaa atccccgctc acgtttttga aaacgtttgt tacaggtgtc
     1021 tgtacggcat tgctggcggt ctgcgtggcc attccgggat ggcagtttct gacacagcca
     1081 tcaccggcgg agcagcaact cagggcgctg ctggcaccac taccggatgt gctttcagca
     1141 gaccagatgg cgcaactcag cagaacaccc tcactgctaa atcatacgtc agagtggata
     1201 accctttccg gtcagcagat aacgcgactg acagaactac cgccggcata caacctgcaa
     1261 cgcagtgcgc agctgctgca gcaactgagg ggacttttcc ccgacaatcc ccgtgttcag
     1321 gaaatggtgg ataactggca taagagcgta aggagccgcg cgctcccgga agaggcgatg
     1381 acgggctgga acgaaggcat ggcccgttta cagcaactgg ctgagcgcct gaaccgtctg
     1441 gatgaacagc ggggaaaata catgacggtc agtgaactga aaacggaggt gtttggcatc
     1501 atgcaggctt ttaaccggca cataccggca gaagagcagt tacgtcgtta tgatgaagcc
     1561 cgtaaccaga atggcagtga acagcagcaa aaacaggctg aaatggcgct gaatcagttg
     1621 attaatcgtt atcagatgga gaacgcaggc aaaccgaaga gccagccata atggctgaga
     1681 taaaacatgg aaatggaaaa tacccccgga agagcgcgca caccatggac aatcgaagat
     1741 gttcgctacc ttgaaacgca ttatggcgtg aagaaaaccc gcgaaatagc ggcatacctg
     1801 aacagaagtg cgacagcagt tcgtgccatg gcgtatctgg tggggataac aacaccagtg
     1861 gctgcggact ggtcagagac agagattgag attattcgca gggactatcc ggcaggcgtt
     1921 gcgcttgctg atatttgtgc ccgtctgaac ggacgagcgc cgaaatccgt gtgctggatg
     1981 gcccgcaggt ttggtctgaa acgcctgggt tactggagtg cagaagaaga tgaaatcctg
     2041 cggcaatatt atccttcaga aggtgtgaaa gtgagccgac gcctttccgg acgtacagaa
     2101 aatgcagtga aactgagagc gcgggagctg ggtgtaaagt tcacgggaaa cgggcgatta
     2161 cgtctctgga cggcagagga gtggaatgtg ttgtccggaa atatggcatc ggggctatcg
     2221 gcactgatga agttactgcc ggggcgttcc cgtagttcag ttcagggggc tatgaggcgt
     2281 ttacagaaaa tgagtgcgca gaaatctgtc aggcagtagc caaaatattc ctgcatcagg
     2341 agaacggcag tggcaataat aagcagcaaa ctgatacata atgcagcttc tgtaaacggt
     2401 aatgatcaag tagggatatt tttatgttga ctggttgtga aaaaatgatc aaaatttaaa
     2461 ttaacctttg catgagatta gaatctatta atttta
//
LOCUS       DAZFLD010000370         2488 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.372, whole genome shotgun sequence.
ACCESSION   DAZFLD010000370 DAZFLD010000000
VERSION     DAZFLD010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2488
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.372"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..935)
                     /locus_tag="ACHKBL_004816"
     CDS             complement(<1..935)
                     /locus_tag="ACHKBL_004816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001178612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO5821551.1"
                     /translation="MQNDDSVTKITWEQLLEEYFFSKNLRPDTEWSYCKVVRVFREFI
                     GDILPGEVTVRDVQRYRRYVLKEQKRREVTWNNKVAHLRAIMGFGIKMKLLPQTENPL
                     VEASVASTEKKKKTLSDNQMLRVYLVVQRFAEAEEQNMVNSRRRNAIYPAWYWLTVLD
                     VLRYTGMRFNQLKHIRVRDVCPDESIIDLKLEGSKTHREWGVPLVAPLKVSMEKLLER
                     ARQCGAGDDDYLFDVCRFSEPISADRYQYCPVRAHQTVRSFFRRLSRECGFPVSPHRF
                     RHTLATRLMKYPERNLHLVKSILGHRSLTTTMGYV"
     gene            complement(1003..>2488)
                     /locus_tag="ACHKBL_004817"
     CDS             complement(1003..>2488)
                     /locus_tag="ACHKBL_004817"
                     /inference="COORDINATES: protein motif:HMM:NF019153.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TraI domain-containing protein"
                     /protein_id="HFO5821552.1"
                     /translation="GMLERLKRLFPVKTECSDVRTDIPGETVPEGYFTPLPADVLVST
                     PFRKRCLKQLWENSSMPVDMYQRLCLDPVHQLLSVSQNLPATAEGHWGYTGGFGDLTL
                     QFTTYAVRLARGRMFPPGATAEEQAAQGSVWNMVVFWSALFYHLPLLAELEGEQADGR
                     CWQPGISVPEGVYRFRFRKMPCQGTEGRQLAALMAGQLLPGEAGAWLATVPGALQNLA
                     GAVWHQHPEMALIRTVLQEAMEEVVSPFAAGDVAEVPVAEVVPVLLQSAAISAESVIQ
                     PLPVVPSPVEDAENEGRTAEQEVDEGNETDADTGMLLTLFSAADLSDEGGQIASGVPP
                     DKESSIELPEESGCVTENQGNRIPETVEKTAEFSLTEDSRVESEFIRWLKEGLREGSL
                     SVNSVDSNVHVVSGYVFLCAPGIFYRFLKESGSGQDRRYVQSLFERAGIHKVRSGERF
                     FQARLYSDAEHSGRYKMVSGYLVKGRFLYGNITVPGDSPLIVFG"
BASE COUNT          598 a          771 c          515 g          604 t
ORIGIN      
        1 tcaacatacc ccatggtcgt tgtaagactc ctgtgtccca gtatggattt gaccagatgt
       61 aaattacgtt caggatattt catcagtctg gttgccagcg tatgtctgaa acgatgtggt
      121 gaaacaggaa aaccacattc tcgcgaaagt cgccggaaaa atgaacgtac ggtctgatgc
      181 gcccgaacgg gacagtactg atatctgtcg gcagagatcg gctcactgaa acgacagacg
      241 tcaaaaagat agtcatcatc acctgccccg cactgacggg cccgctccag cagtttttcc
      301 atcgatacct tcaggggagc caccagagga acgccccatt ccctgtgagt tttacttccc
      361 tccagcttaa gatcaattat gctttcatcc ggacagacat ccctcacacg gatatgcttc
      421 agttgattaa agcgcattcc ggtataacgc agaacatcca gaactgtcag ccagtaccag
      481 gcagggtata tagcattgcg tcgacgacta ttcaccatgt tctgctcttc cgcttctgca
      541 aacctctgca caaccaggta aaccctgagc atctgattgt cactcagtgt ctttttcttt
      601 ttttcagtac tcgcaactga tgcttccacc aacgggtttt ctgtctgagg cagcagtttc
      661 atcttgatcc cgaatcccat aattgcccgc aagtgggcca ccttgttatt ccaggtcacc
      721 tccctgcgct tctgctcttt caggacataa cgccggtatc gctgaacatc acggacagtc
      781 acctcccccg gcaaaatatc accgataaat tccctgaata ccctgacaac tttgcagtag
      841 ctccactctg tatccggccg aagattcttt gaaaaaaagt actcttccag cagttgttcc
      901 caggtgattt ttgtgacgga atcgtcattc tgcattatat attccccttg tttactgtgc
      961 tcccgcactc tccggtcaaa ttctgcacat catcgccttt actcacccga aaacaataag
     1021 cgggctgtct ccgggaaccg taatattccc gtacagaaac ctgcccttaa ccagatatcc
     1081 cgaaaccatt ttgtaacgtc cggaatgttc tgcgtcacta taaagacggg cctgaaaaaa
     1141 acgctctcct gaacgtacct tatggatacc tgcccgttca aagagcgact gaacataacg
     1201 acgatcctgc ccggaaccac tctccttcag aaaacgataa aaaatccccg gggcacacag
     1261 aaaaacatat ccggaaacaa catgaacatt tgaatcaacg ctgttaaccg aaagtgaccc
     1321 ctccctcagc ccctctttta accatctgat aaattcactc tctaccctgc tatcctctgt
     1381 cagggaaaac tctgccgttt tttccactgt ttccgggata cggtttccct gattttctgt
     1441 tacacatccg ctctcttccg gtaactcaat actgctttct ttatccggcg gcacgcctga
     1501 cgctatctgc cctccctcat ccgacaaatc agcagccgaa aacagcgtaa ggagcattcc
     1561 ggtatcagca tcagtttcgt tcccctcatc cacctcctgt tccgctgtcc ggccctcatt
     1621 ttcggcatcc tccactggcg acggtacaac cggcaacggc tgaatgacgc tttctgctga
     1681 aattgctgct gactgcagca gtaccggaac aacctcagcc accggaactt cggccacatc
     1741 tcctgcagcg aagggactca cgacttcctc catagcttcc tgtaatactg tacgtatcag
     1801 cgccatttca ggatgctgat gccagactgc tccggcaaga ttctgtaatg cccccgggac
     1861 agtggcaagc caggctcccg cctctccggg cagcagttgt cccgccatca gggctgccag
     1921 ttgccgtccc tccgttccct ggcagggcat cttcctgaac cggaaccggt atacgccctc
     1981 cggtacagat atccccggct gccagcatct gccgtcggcc tgttcgcctt ccagttccgc
     2041 cagcaacggc aggtgataaa acagagccga ccagaacacc accatgttcc acacactccc
     2101 ctgcgcggcc tgctcttctg ctgtcgctcc cggtggaaac attctgcctc tggccagccg
     2161 gacagcatac gtcgtgaact gcagggtcag gtcaccaaaa ccaccggtgt aaccccagtg
     2221 accttctgct gtggccggca gattctgact gacagacaac aactgatgca caggatcaag
     2281 acaaaggcgc tgatacatat ccaccggcat ggaactgttt tcccataact gtttcagaca
     2341 ccttttcctg aacggtgtgg ataccagtac atcagccggc aatggtgtaa aatacccctc
     2401 cggcaccgtt tccccgggaa tatccgtccg gacatcagag cattctgtct tcacaggaaa
     2461 taaccttttc aggcgttcaa gcatgccc
//
LOCUS       DAZFLD010000371         2478 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.373, whole genome shotgun sequence.
ACCESSION   DAZFLD010000371 DAZFLD010000000
VERSION     DAZFLD010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2478
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.373"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(104..1387)
                     /locus_tag="ACHKBL_004818"
     CDS             complement(104..1387)
                     /locus_tag="ACHKBL_004818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000425776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VRR-NUC domain-containing protein"
                     /protein_id="HFO5821553.1"
                     /translation="MEMPDLPETPTSDIPTGANNQPWDCTTTGVEMVEKQSRLPTTEN
                     RQYLHIKVEYAMRFPQLSAQIRRKGKTYQMSLKQTVMSGLIRVDEIARNYCWYYKAEV
                     AFDMRTTPPRPFLSSTLLEKDGAGRRHTTNPFPVPGRGIYRRPDVIIVKNKEDRWPGL
                     AGPDTEGNMHSDNLARLVEVKFPGDRLYEDQYEEYMQIVGRNRTRMTILEIHDCRPEE
                     HQWVDQVVNVTMKAWQISGAKYWPLALYPPVFMPRPPSTPVAARIEPWTYAGKVVSDL
                     SEGAVRGVAEGWNALSKETQLVLNSAASWLNDSGEWVYRQGSEVWVWASQTGKSVKSW
                     TNTQIKAAWTEIQHVTDMTLDMLKQVDWVQVLTNVAKTVGVILVAVGVGGLVVTLGIP
                     EAIIGAFLLIVRLAISAWGTLATILGTGTAVLAVS"
     gene            complement(1387..>2478)
                     /locus_tag="ACHKBL_004819"
     CDS             complement(1387..>2478)
                     /locus_tag="ACHKBL_004819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2345 domain-containing protein"
                     /protein_id="HFO5821554.1"
                     /translation="FANSKLRMEDRRGEEHIKLATEYGKTQLNSGHLVDSQGQLRGKG
                     TELRTDEWGTLRAGKGLFVAADAQAKAQGEALDMDAALKEIDRLNQQLQQLENAAERA
                     QALKADIDSQIQMFAQRLKPLNEAVLFSAPEGIALTSGEDMQMSAARNVAVNAGGDIS
                     TGTMGNLTALAGERLGLFARTGQLSVKSGEGPVEVQAQNGSMRLFAEKKLTISSESDI
                     SFTGKKRITLIGGGSYLKLEAGKVEYGTMATCMRRVKRTMTAAAQSMPMAFPRMSSSH
                     IYSAIYRLLDKHGNILVNTPCRLTTPSGQTVTGYSDNEGRSVPVYTRQQEDVELHFFV
                     KKPQPEETMWFVGETDTQQLETELRESQS"
BASE COUNT          488 a          756 c          607 g          627 t
ORIGIN      
        1 gattgactgc cggactgccg tctttgtatt tcacggaggc ttccggcagc catttctcga
       61 tgtagttcag ctcaaaatca tgcaattcca tacctgctcc tgtttaacta actgccagta
      121 ccgccgtacc cgtaccgaga atcgtcgcca gtgttcccca ggcgcttatt gccagacgaa
      181 caatcagtaa aaacgcgcca ataatggctt ccgggatacc cagcgttacg accagtccgc
      241 cgacacccac agcaaccaga ataacgccga ccgttttcgc gacgttggtt aaaacctgta
      301 cccagtccac ctgtttcagc atatcaagcg tcatatcggt gacgtgctgt atttctgtcc
      361 atgcggcctt aatctgggtg ttggtccagg atttcaccga cttaccggtt tggctggccc
      421 agacccagac ctcacttccc tgccggtaaa cccactcacc gctgtcgtta agccagctgg
      481 ctgctgaatt caggaccagt tgcgtctctt ttgacagggc attccagcct tcagccacac
      541 cacgcaccgc cccctcactg aggtcagata ctactttacc agcgtaagtc cagggctcta
      601 ttctggcagc aacgggggtt gatggcggac ggggcataaa gactggcggg taaagagcca
      661 gcggccagta tttcgcgccg ctgatctgcc aggctttcat cgtcacgttg accacctggt
      721 caacccactg gtgctcttcc gggcgacagt cgtgaatctc cagaatggtc atgcgggttc
      781 ggttcctgcc cacaatctgc atatactctt catattggtc ttcatacagc ctatctcccg
      841 gaaacttcac ctccaccagg cgggccaggt tatcgctgtg catattgcct tcggtatccg
      901 gccccgccag tccgggccag cggtcttcct tatttttaac aataatcacg tccgggcggc
      961 ggtaaatgcc cctgccgggc acagggaaag gattggtcgt gtggcgacgg ccagcaccgt
     1021 ctttttccag cagcgtactg ctcagaaaag ggcgtggcgg cgtggtgcgc atatcaaacg
     1081 ccacctccgc cttataatac cagcagtaat tgcgggcgat ttcatccacg cgaataagac
     1141 cactcataac ggtctgctta aggctcatct ggtaagtttt acctttccgg cggatttgtg
     1201 ccgacagttg cggaaaacgc atggcatatt ccacctttat atgcaggtac tgacggtttt
     1261 ccgtggttgg cagacgcgac tgcttttcga ccatttcgac acccgtcgtg gtgcagtccc
     1321 acggctggtt gttcgcccca gtgggaatat cagatgtcgg tgtctcgggt aaatcgggca
     1381 tttccattat gactggctct ccctgagttc ggtttccagt tgctgcgtat cggtttcccc
     1441 cacaaaccac atcgtttctt cgggttgtgg ttttttaaca aaaaaatgca gttccacatc
     1501 ctcctgctgg cgggtgtaaa ccggcacgga gcggccttcg ttatcgctat accccgttac
     1561 tgtctgacct gatggagtgg tgaggcgaca gggggtattg accagaatat tgccgtgttt
     1621 gtccagcaac ctatagatag ccgagtagat atgcgaagaa ctcatacggg ggaatgccat
     1681 cggcatactc tgcgcagccg ccgtcatcgt ccgcttcacc cgacgcatac aggttgccat
     1741 cgtgccatat tcaaccttcc cggcttccag tttcaggtaa ctgccaccgc caatcagcgt
     1801 aatgcgtttc ttgccggtaa acgagatatc actttctgac gaaatggtca gcttcttctc
     1861 agcaaacaac cgcatgctgc cgttttgcgc ctgtacctct actggcccct ccccggattt
     1921 gacgctcagt tgtccggtcc tggcaaaaag ccccagcctt tctccggcca gcgcggtcag
     1981 gttgcccatt gttccggtac tgatatcacc tccggcgttg acagccacat ttcgtgctgc
     2041 actcatctgc atatcttcac cgctggtcag ggcaatgcct tccggagccg agaaaagcac
     2101 ggcttcattg agcggtttca gccgctgcgc aaacatctgt atctgactgt caatatcagc
     2161 cttcagcgcc tgtgcccgtt ctgcggcatt ttccagttgc tgcagttgct gattaagcct
     2221 gtcgatttcc ttcagcgcgg catccatgtc cagtgcctcg ccctgtgctt tcgcctgcgc
     2281 atccgcagca acaaaaagtc ccttccccgc tctcagggtt ccccactcat cggtacgcag
     2341 ctctgtgcct ttaccccgca gttgcccctg gctgtccacc agatgtccgc tgttaagctg
     2401 ggtcttaccg tactccgtgg cgagtttgat atgctcctcg ccgcgccggt cctccatccg
     2461 cagttttgag ttagcgaa
//
LOCUS       DAZFLD010000372         2474 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.374, whole genome shotgun sequence.
ACCESSION   DAZFLD010000372 DAZFLD010000000
VERSION     DAZFLD010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2474
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.374"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..317
                     /locus_tag="ACHKBL_004820"
     CDS             <1..317
                     /locus_tag="ACHKBL_004820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5821555.1"
                     /translation="LLNYHIACPFPAAQIRFPAFLPRRSENPESRIFLSLTQRHLLSV
                     QHVVLHSAAHDTIYCVISLTCDASRRGGKQRYDYFPSVVHFRKVVIFRKVGSDGNGSP
                     VI"
     gene            461..874
                     /locus_tag="ACHKBL_004821"
     CDS             461..874
                     /locus_tag="ACHKBL_004821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5821556.1"
                     /translation="MFFIENEGQAVARTDYWQSVQAQAGYVYLSWNAGAARLLVPDAA
                     KHLLREMRGAEYVIISKGTLDGRDALELIFEDGSDAPFVIHMLSEQCDRLLPENNQGG
                     GFVVTVWTRGGNQLRYPGKYRVVENLPDVSPWSEH"
     gene            876..1655
                     /locus_tag="ACHKBL_004822"
     CDS             876..1655
                     /locus_tag="ACHKBL_004822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001164196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFO5821557.1"
                     /translation="MQHLPAPIHHARDAAQLPVAIDYPAALALRQMSMVHDELPKYLL
                     APEVSALLHYVPDLRRKMLLATLWNTGARINEALALTRGDFSLAPPYPFVQLATLKQR
                     TEKAARTAGRMPAGQQTHRLVPLSDAWYVSQLQTMVATLKIPMERRNKRTGRTEKARI
                     WEVTDRTVRTWIGEAVAAAAADGVTFSVPVTPHTFRHSYAMHMLYAGIPLKVLQSLMG
                     HKSISSTEVYTKVFALDVAARHRVQFSMPESDAVSMLKRIP"
     gene            1835..>2474
                     /locus_tag="ACHKBL_004823"
     CDS             1835..>2474
                     /locus_tag="ACHKBL_004823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001735283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFO5821558.1"
                     /translation="MPVIANTHPKGGVGKTTSSVNIVGEMKSDTVDLDTHTGLSIILG
                     LRPEGKEISVKVPKTVDELIEIMTPYKNSDKTLLIDCGGFDSDLTRTAIAFADCVIVP
                     SKDSLTERIGLMHFDGVLDEISSIMGTDITAHLYLCKVNPNKKKFPKLDAILPSFKHL
                     KLMKSRISARAEFEDVIETGMGITESVHGRYSAGGKEVIALIEEINHLIENNK"
BASE COUNT          594 a          601 c          670 g          609 t
ORIGIN      
        1 tgttgcttaa ttatcatatc gcctgcccgt ttcccgccgc gcagatccgg tttccggctt
       61 ttctgccgcg cagatccgaa aaccctgagt cacggatctt cctctccctc acacaacgcc
      121 atctcctgtc agtacaacat gtcgtgctgc attcagctgc tcatgacact atatattgtg
      181 ttatttccct gacctgtgat gcgtcgcgca ggggcggaaa acagcgatat gattatttcc
      241 ccagcgtggt acacttccgg aaagttgtga tattcaggaa agtcggatct gacggaaacg
      301 gctctccggt aatttaacgg cgtggttata tggatgcttg ttatcatggt gatgatgata
      361 acggcatgat gttatcagac ggcgtgacgg taagggcagt gatgatggat gacgttatcg
      421 catgaccgtc cctgcccgga aaagaaaaaa ggagtcaccc atgtttttta ttgagaatga
      481 aggtcaggct gtcgccagaa cggattactg gcagtctgta caggcgcagg ccggatatgt
      541 ctacctcagc tggaatgccg gcgcagccag gctgcttgtc ccggatgcgg caaaacattt
      601 actcagggag atgcgggggg ctgagtacgt catcatcagt aagggaacgc tggatggccg
      661 cgatgcgctg gaactgatat ttgaagacgg cagcgatgcg ccgtttgtga tccacatgct
      721 gagtgagcag tgcgatcgcc tgctccccga aaacaaccag ggagggggtt ttgttgtcac
      781 cgtctggacg cgtggcggta accagctccg ttatccggga aaataccggg ttgtggaaaa
      841 cctgcccgac gtttccccgt ggagtgagca ctgatatgca gcacctgccg gcaccgatcc
      901 accatgcccg ggatgccgct cagcttcctg ttgccatcga ttatccggca gcgctggcac
      961 tccgccagat gtcgatggtt catgatgaac tgccaaaata cctgctggcc cctgaagtga
     1021 gcgccctgct ccattacgtg ccggatctgc gccgcaagat gctgctggcc acactgtgga
     1081 acaccggtgc gcgcattaat gaagcactgg cgctgacgcg gggggatttt tcgcttgcgc
     1141 ctccgtatcc gtttgtgcag cttgcgaccc tgaaacaacg gacggaaaaa gccgccagga
     1201 cggcaggaag aatgcccgcc ggtcagcaga ctcaccggct ggttccgctc tccgacgcct
     1261 ggtacgtcag ccagctgcag acgatggtgg ccacactgaa aatccccatg gaacggcgta
     1321 ataaacgaac aggcaggaca gagaaagcgc ggatctggga agtgacggac agaacggtca
     1381 ggacctggat tggggaggcg gttgccgccg ctgctgctga cggtgtgacg ttctctgtcc
     1441 cggtcacgcc acatacgttc cgccattcct atgcgatgca catgctgtat gccggtatac
     1501 cgctgaaggt cctgcagagc ctgatggggc ataagtccat cagctcaaca gaggtctaca
     1561 cgaaggtgtt tgcactggat gtggctgcac ggcaccgggt gcagttttcg atgcctgagt
     1621 ccgatgcggt gtctatgctc aaaagaattc cttaagctct gactttttta aatccctttc
     1681 ggatatctca tggatatatt atcggtattt catattctgt tgacatgcag gaccggtata
     1741 gagtaaaaaa acatctattg agtacctctt ggatatccga aaggtgtctt aggggggctt
     1801 tatgggtatt acatgtggat tctggagata cactatgcct gttattgcta atacgcatcc
     1861 caaaggtggt gtgggtaaga caacttcatc cgtaaacatt gttggtgaaa tgaagtctga
     1921 tacagttgat ctggatactc ataccgggct ttctatcatt ctggggctga gacctgaagg
     1981 aaaagaaatt tccgtgaaag tacccaaaac agtggatgag ttaatcgaaa ttatgactcc
     2041 ctacaagaac agcgataaga cgttacttat tgactgtggg ggatttgact cagatcttac
     2101 ccgtactgcg attgcctttg cagactgcgt tattgtccct tcaaaagact ccctgactga
     2161 acgtattggt ctgatgcatt ttgatggagt actggacgaa atcagctcta tcatggggac
     2221 cgatataact gctcacctgt atctctgtaa agttaatcct aacaagaaga aattccctaa
     2281 gcttgatgca atcctgccat cgttcaaaca tctgaagctg atgaagagcc gcatttctgc
     2341 tcgtgcagaa tttgaggatg tcattgagac tgggatggga atcactgagt ctgttcatgg
     2401 gcgctattcg gcaggaggta aggaagttat tgccctgatc gaagaaatca atcatctgat
     2461 tgaaaataac aaac
//
LOCUS       DAZFLD010000373         2466 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.375, whole genome shotgun sequence.
ACCESSION   DAZFLD010000373 DAZFLD010000000
VERSION     DAZFLD010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2466
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.375"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(38..262)
                     /locus_tag="ACHKBL_004824"
     CDS             complement(38..262)
                     /locus_tag="ACHKBL_004824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5821559.1"
                     /translation="MKKLLVTVKPFQGTIPFRILQRGRVLVEGSFSGKCTQLYSRTFQ
                     VNATNEELTVECTMNAAKCRMVSAALQPVC"
     gene            complement(286..753)
                     /locus_tag="ACHKBL_004825"
     CDS             complement(286..753)
                     /locus_tag="ACHKBL_004825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HFO5821560.1"
                     /translation="MNRVLCVVIIALLVACGALSLGLNHYRDNAITYKAQRDKKAREL
                     ELANATITDMQVRQRDVAALDAKYSRELADARAENETLRADVAAGRKRLRINATCHGT
                     VREATGTSGVDNATGPRLADTAERDYFILRERLMTMQKQLEGAQEYIRTQCIN"
     gene            complement(1052..1585)
                     /locus_tag="ACHKBL_004826"
     CDS             complement(1052..1585)
                     /locus_tag="ACHKBL_004826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFO5821561.1"
                     /translation="MNAKIKYGLSAAVLALIAAGASAPEILDQFLDEKEGNHTTAYRD
                     GAGIWTICRGAIMVDGKPVIPGMKLSKEKCDRVNAIERDKALAWVEKNIRVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRINAGDRRGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     ISRRDQESALACWGIDR"
     gene            1711..2031
                     /locus_tag="ACHKBL_004827"
     CDS             1711..2031
                     /locus_tag="ACHKBL_004827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001063217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5821562.1"
                     /translation="MNNNDNNLRKEFLQIMNENVKSELKALIPDNSETTQAILSEPYG
                     MLSTETLDIIITTLTPLMLQHLKHNINKWFNDELSHPGCSWDKNFACLQKKRLFNKLS
                     LKFR"
     gene            complement(2024..>2466)
                     /locus_tag="ACHKBL_004828"
     CDS             complement(2024..>2466)
                     /locus_tag="ACHKBL_004828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5821563.1"
                     /translation="FRHEGNDAKGSFTKIGPFFLKVDFSDQEWECNRPSFVVSGDDGK
                     ITISENGKVTPPSHQHCETLIEFAIDYLKNNKKQGLMKRIGRCMGYLQVAAEIEALVS
                     GADKDAVVRETVLRDFNTPPFKTEPDDWIQPGLTYLKGRFGAVI"
BASE COUNT          641 a          667 c          523 g          635 t
ORIGIN      
        1 tacggcgaca ataccgcgcc atggataata aggtcgctca acacactggc tgtaatgcag
       61 ccgataccat gcggcattta gcggcattca tcgtacactc aacggttagc tcttcattcg
      121 tggcattcac ctgaaaggtc cgggagtata attgcgtaca tttaccactg aacgaacctt
      181 caacaagaac acgaccacgc tgcaaaatac ggaacggaat tgttccctga aaaggcttta
      241 cggttaccag taatttcttc atgcattctc cggataacaa aaatactagt taatacactg
      301 agtgcggata tattcctgcg ccccttccag ctgcttctgc attgtcatca accgttctct
      361 gaggatgaaa taatcccgtt cagcggtgtc tgccagtcgg gggccggttg cattatccac
      421 gccggaggtg ccggtggctt cacgcacggt accgtggcag gtggcgttga tccgcaggcg
      481 cttacgacca gcggcaacat cagcacgcag agtttcattt tcagctctcg catcggctaa
      541 ttccctcgag tattttgcat cgagcgcagc aacatcgcgc tggcgcacct gcatatcagt
      601 aatggttgcg tttgccagct ccagctctct ggctttttta tcgcgctgcg ctttgtaggt
      661 gatggcgtta tcgcggtaat gattcagccc cagactaagc gcaccacagg ccaccagcag
      721 ggcaatgatg accacacaca gaacgcggtt catttcacca ccagcgtatc tgaccgatga
      781 aataaccgga ggccataatc acaaacacca gccagataag gatgaacttc caggtggata
      841 atttttcagc catcactcga atctcccgaa tcagtttgct aaaattaaac acactttctc
      901 ctttgacttt tccggagtca ggaaacacaa aaccccgctt gctaccaaca aacggggttt
      961 ttacttttat tcacttaggt tttgccagtt cgcaggattt cgtgttatcc gtccgcgttg
     1021 gccaacgtca tttttcagca aaatattctg cttatctgtc gattccccag cacgccagcg
     1081 cgctctcctg gtcacgacgg gatatctggc cgtaacagtt gtttgagcgg atacggcagt
     1141 ctctgccacc gtccttaatc caccagcgaa tcgcttcaca cgctcccctg cgatcgcctg
     1201 cattaattcg tttataaaac gtcgacggga aacacttacc ggggccaatg ttatacgggc
     1261 agaatgacgc aatacccgct ttctggggtt cggtcagtgg cactctgatg tttttctcca
     1321 cccatgccag cgccttatcc cgttcgatag cgttaacccg gtcgcatttt tccttcgaca
     1381 gcttcatgcc aggaatcaca ggcttaccat ccaccatgat ggcaccccgg cagatggtcc
     1441 agatacccgc accatcacgg tatgccgtgg tgtggttgcc ttccttttca tccagaaact
     1501 ggtcgaggat ttcaggcgca gaagcccctg cggcaatcag cgccagaacg gcagctgaca
     1561 ggccgtattt gatttttgcg ttcatggata tttatcagga tttatagctc tcttattcct
     1621 ggatatgtca gatatataat ccggtactca cgatgaatat aatcaacagg caccaggaca
     1681 cacccggtaa caacaaatga gattttataa atgaataata atgacaacaa tttacgcaaa
     1741 gagtttttgc agataatgaa cgaaaatgtt aaatcagaat taaaagcgct tatcccggat
     1801 aatagcgaaa ccactcaggc cattctctca gagccttacg gcatgctttc aacagaaaca
     1861 ctggatatca ttatcaccac attaacacca ttgatgcttc agcatctgaa gcacaacatt
     1921 aacaaatggt ttaatgacga actcagtcac ccgggttgtt catgggataa aaactttgcc
     1981 tgtctgcaaa aaaaacggct cttcaataaa ctatcgctta agttcagata accgccccaa
     2041 accgcccttt cagataagtc agccccggct ggatccaatc atctggctca gtcttaaagg
     2101 gtggagtatt gaaatcacga agaacggtct cccgcacaac tgcatcctta tcagcaccac
     2161 tgaccagcgc ttcaatctca gcggctacct gcagataccc catgcaacga ccaatgcgct
     2221 tcattagtcc ctgcttttta ttgttcttca ggtaatcaat ggcaaattca atgagcgtct
     2281 cacagtgctg gtgcgatggc ggtgttactt ttccattttc tgagatagtt attttcccgt
     2341 catcaccgga tacaacaaag gatggccggt tacactccca ttcctggtca ctgaaatcta
     2401 ccttcagaaa gaacggcccg atttttgtaa aggaaccttt ggcatcgttc ccctcatgac
     2461 ggaata
//
LOCUS       DAZFLD010000374         2461 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.376, whole genome shotgun sequence.
ACCESSION   DAZFLD010000374 DAZFLD010000000
VERSION     DAZFLD010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2461
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.376"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1428
                     /gene="yihF"
                     /locus_tag="ACHKBL_004829"
     CDS             <1..1428
                     /gene="yihF"
                     /locus_tag="ACHKBL_004829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 family protein"
                     /protein_id="HFO5821564.1"
                     /translation="IRKSATGVIVALAVIWGGGTWYTGTQIQPGIEKFIKDFNDAKKK
                     GEHAYDMTLSYKNFDKGFFNSRFQMQMTFDNGAPDLNIKPGQKVAFDVDVEHGPLPIT
                     MLMRGNVIPALAAAKVKLVDNELTQPLFIAAKNKSPMEATLRFAFGGSFSTTLDVAPA
                     KYGKFSFGEGQFTFNGDGSSLSNLDIEGKVEDLVLELSPLNKVTAKSFTIDSLTRLEE
                     KKFPVGESESKFNQINIINQGEDVAQIDAFVAKTRLDRVKDKDYINVNLTYELDKLTK
                     GNQQLGSGEWSLIAESIDPSAVRQFVIQYNIAMQKQLAAHPELANDEVALQEVNAALF
                     KEYLPLLQKSEPTIKQPVRWKNALGELNANLDISIADPAKSSSSTNKDIKSLIFDVKL
                     PLNVVTETAKQLNLSEGMDAEKAQKRADKQISGMMTLGQMFQLITIDNNTASLQLRYT
                     PGKVVFNGQEMSEEEFMSRAGRFVH"
     gene            complement(1469..2401)
                     /gene="yihG"
                     /locus_tag="ACHKBL_004830"
     CDS             complement(1469..2401)
                     /gene="yihG"
                     /locus_tag="ACHKBL_004830"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HFO5821565.1"
                     /translation="MANLLNKFIMTRILAAITLLLSIVLTILVTIFCSVPIIIAGIVK
                     LLLPVPVIWRKVSRFCDFMMYCWCESLAVLLHLNPHLQWEVHGLEGLSKKNWYLLICN
                     HRSWADIVVLCVLFRKHIPMNKYFLKQQLAWVPFLGLACWALDMPFMKRYSRAYLLRH
                     PERRGKDVETTRRSCEKFRLHPTTIVNFVEGSRFTQEKHQQTHSTFQNLLPPKAAGIA
                     MALNVLGKQFDKLLNVTLCYPDNNRQPFFDMLSGKLTRIVVHVDLQPISDELHGDYIN
                     DKSFKRHFQQWLNSLWQEKDRLLTSLMSSQRQDK"
BASE COUNT          758 a          514 c          549 g          640 t
ORIGIN      
        1 atacgtaagt cagctacagg tgttattgtt gcgttagccg taatctgggg tggtggcaca
       61 tggtacacag gtacgcaaat tcagcctggt atcgaaaagt ttattaaaga ttttaacgat
      121 gctaaaaaga aaggtgaaca tgcctacgat atgacgttaa gttataaaaa ttttgacaaa
      181 ggctttttta attctcgttt tcaaatgcaa atgacatttg ataacggtgc acctgatctc
      241 aatatcaaac ctggccagaa agttgcgttt gatgtggatg ttgagcacgg tccgttaccc
      301 atcacaatgt taatgcgtgg taatgtcatc cccgcactgg cagcggcaaa agtgaaatta
      361 gtggataatg aactgacaca accgctattt atcgccgcaa aaaataaatc tcctatggaa
      421 gcgacattgc gattcgcgtt tggtggctca ttctccacga cattagatgt tgcccctgca
      481 aaatatggaa agttttcttt tggtgagggc cagtttactt ttaatggtga tggtagttca
      541 ttgtctaatc tggatattga aggcaaagtc gaagatcttg ttctggaatt atcgccattg
      601 aataaagtaa cggcgaaaag ttttaccatt gattctctaa cgcgattaga agaaaagaaa
      661 tttccggttg gcgaaagcga gtcgaaattc aatcagatta acattatcaa tcagggagaa
      721 gacgttgccc aaatcgatgc tttcgttgca aaaaccaggc tggatcgcgt taaagacaaa
      781 gattatatca atgtcaatct gacctacgaa cttgataagt taacaaaagg gaatcagcaa
      841 ctcggtagtg gtgagtggtc attgattgct gaatctattg atccctcagc ggtgcgccaa
      901 tttgtcatcc agtataacat tgcgatgcag aagcagcttg ctgcacaccc tgagttagca
      961 aacgatgaag ttgctctgca agaagtgaat gctgcattgt tcaaagagta tttaccgtta
     1021 ttacaaaaaa gtgagccgac cattaaacaa ccggtaagat ggaagaatgc actcggcgaa
     1081 ctaaatgcca atctggatat cagtattgcc gacccagcca aatcctcatc atccacaaac
     1141 aaagatatta aatcgctcat ttttgatgtg aagttaccgc ttaatgttgt cacagaaacc
     1201 gcaaaacagc ttaatttatc tgaaggaatg gatgctgaaa aagcgcaaaa gcgggctgat
     1261 aaacaaatca gcgggatgat gacattaggt cagatgtttc agttaatcac gattgacaac
     1321 aataccgcct cgctgcaact gcgttataca ccgggtaaag ttgtttttaa cggacaggag
     1381 atgagcgaag aagaatttat gtctcgtgcc ggacgttttg ttcattaaaa tactcactcg
     1441 cctctgctca ttcagaggcg agcaaaattt acttgtcctg ccgctgtgat gacatcaacg
     1501 acgtcagaag ccggtctttt tcctgccaca gtgagttcag ccattgctga aaatggcgct
     1561 taaagctttt gtcattgatg tagtcgccat gtaactcatc ggaaataggc tgcaaatcca
     1621 catgtacgac aattcgcgtc agtttaccgc ttaacatatc gaagaacggc tgacggttat
     1681 tgtccggata acacagcgta acattaagca gtttatcgaa ttgtttaccc agtacattta
     1741 gcgccatcgc aatgcctgcc gcctttggtg gcaacaagtt ttgaaaagtg gagtgcgttt
     1801 gctgatgttt ttcttgcgtg aaacgggagc cttcaacgaa attgacaatg gtggtggggt
     1861 gcaggcgaaa cttttcacaa gaacgccggg tggtttcaac atctttacca cgtcgttccg
     1921 gatgacgtaa taaataagcg cgggaataac gcttcataaa cggcatatcc agcgcccagc
     1981 atgccaggcc aagaaatggc acccaggcaa gctgctgctt gaggaaatat ttattcatcg
     2041 gaatgtgctt acgaaacagc acgcacagta cgacaatatc tgcccaacta cggtggttac
     2101 aaataagcag ataccagttc ttcttactta acccttccag cccgtgaact tcccattgca
     2161 ggtgtgggtt cagatgcagt aataccgcca gactttcaca ccagcaatac atcataaaat
     2221 cacaaaaacg cgaaaccttt cgccaaatga ctggcacagg tagcaatagt tttacaatcc
     2281 cggcaatgat gatcgggacg gagcaaaaaa tagtgaccaa aatggtcaat acgatactca
     2341 gcaaaagggt tatcgcagcg agtattctcg tcataatgaa tttattcaaa aggttagcca
     2401 tagacagtgc gctaaagaag cgcaaggcgc cgattttagc agaaaacgcc ccaaataacg
     2461 g
//
LOCUS       DAZFLD010000375         2450 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.377, whole genome shotgun sequence.
ACCESSION   DAZFLD010000375 DAZFLD010000000
VERSION     DAZFLD010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2450
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.377"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..296
                     /locus_tag="ACHKBL_004831"
     CDS             <1..296
                     /locus_tag="ACHKBL_004831"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5821566.1"
                     /translation="APEASARLKPGDAQKSSTAHKKQPHHLPSGSPCSPCFSRKTRLT
                     DGEFCLSTFNCKGFLRKVTTVHAIKRQPQALQGAMFLLNPSTVFPLNYLITSI"
     gene            942..1214
                     /locus_tag="ACHKBL_004832"
     CDS             942..1214
                     /locus_tag="ACHKBL_004832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000451333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HFO5821567.1"
                     /translation="METYIRTRVDDVLKSQFETILQDCGLSVSAALRLFAENVVRNEG
                     LPFEITRKPSARLRESIRQTEELMAQGRPGFENVGELIASMNNGEQ"
     gene            1201..1506
                     /locus_tag="ACHKBL_004833"
     CDS             1201..1506
                     /locus_tag="ACHKBL_004833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000535377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system YafQ family
                     toxin"
                     /protein_id="HFO5821568.1"
                     /translation="MGSNKRAPLPYRTDRTKNFEKAWKRYNKSGRYDMQAAAVAMNLV
                     TNRNPVPAEYRDHELTGEWAGFRELHIGGDFLLVYRVDEKNEIIYFTDLGTHAELFE"
     gene            2245..>2450
                     /locus_tag="ACHKBL_004834"
     CDS             2245..>2450
                     /locus_tag="ACHKBL_004834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001402899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5821569.1"
                     /translation="MRDKTREAMRLFLGGRCYTAEKLEKDYLAEVANYSDDRWEAPQR
                     AARLAAAVKRYKTSEMLRFIFATIA"
BASE COUNT          659 a          591 c          553 g          647 t
ORIGIN      
        1 ctgcgccgga ggcttccgca cgtctgaagc ccggcgacgc acaaaaaagc agcacagcac
       61 acaaaaaaca gcctcatcat ctgccttccg gctcgccttg ctccccctgt ttttcacgca
      121 aaacacgtct cacagacggg gaattctgtt tatccacatt taactgcaag ggatttctcc
      181 gtaaggttac aaccgttcat gctataaagc gccagccgca ggcgttacag ggtgcaatgt
      241 ttcttttaaa ccccagtact gtatttcctt taaactactt aattacttcc atttaaaaag
      301 aaaacctatt cactgcctgt cctgtggaca gacatatatg cacctcccac cgcaagcggc
      361 gggccccgac cggagccact ttagttacaa cactcaaata caagcaccga aaaaaactag
      421 gtccaacgca gaactgaaac cacaacgccc ctccctcata actgaaaagc ggccccgccc
      481 cggcccaaag ggccggaaca gagtcgcttt taattatgaa tgttgtaact aaacatcatc
      541 atcgctgtca gtcttctggc tggatgttct cagtacacgc tcgtaagcgg ccctcacggc
      601 ccgctaacgc ggagatacgc cccgacttcg ggtaaaccct tgtcgggacc actccgaccg
      661 cgcacagaag ctctgtcatg actgaaagcg ggtatggctt agcagggctg gggatgggta
      721 aggtgaaatc tgtcaatcag ttccggctta cgccgggctt cggcggtttt actccgctgc
      781 catatgcaac aacggagtac cgccttacat gcgctaacgc gcataaatcc ccgttcctca
      841 gtggaacgaa aactcacgct agctgggaaa tatgcacctt gttttgtagt ccattatgga
      901 ctacattagc aacaccaata tttgattaat gaggtgatga gatggaaaca tatatccgta
      961 ctcgtgttga tgatgttctt aaaagccaat ttgaaactat cctgcaagat tgcggattat
     1021 ctgttagcgc tgcattgcgg ttatttgctg aaaatgttgt ccgtaatgaa gggttgccat
     1081 ttgaaattac ccgtaaacca tcagctcgtt tgcgtgaatc catccgtcaa accgaagaac
     1141 tgatggcaca agggcggcca ggttttgaaa atgttggtga gctgattgcg agtatgaaca
     1201 atggggagca ataaacgcgc accgttgcct taccggacgg atcgcactaa gaactttgaa
     1261 aaggcatgga aacgctataa caaaagcgga cgctatgata tgcaggcggc tgccgtagca
     1321 atgaaccttg tgactaatcg taatcctgtt ccggctgaat atcgagacca tgaacttact
     1381 ggagagtggg ctggtttccg agaattgcat attgggggcg attttctact ggtttatcgc
     1441 gtcgatgaaa aaaacgagat tatttatttc actgatcttg gtactcatgc tgaactcttt
     1501 gaataaaaat tgcgtcacgc aatgactgcc ctccacgctc acggtatcaa gaaatcagtc
     1561 attgtcctgt actgggggca gtttggatat tggaaattat tgtacgttag gagcattttt
     1621 taatcgatac tgataagtaa ctgattgatt tatcgtgatc agctatcgcc tttaagttat
     1681 tgcttttctc ttatcgcatt taggttatct catttctcta aagatctgcc aaagaccgta
     1741 tagcgttgga gtacacccta ccgcaatagc gcaaccgtac aagggggttc gcgcccattt
     1801 cctctaaaag tctcaccttg cttaggggtc agtttggata tagagaatta ttgtacgttc
     1861 agcgcaacaa acttaatgct aatgtatgca tacattagca ttaaccacac atttaagact
     1921 ggggtttgag gctcagtgga acaataaccg ccgttaagcg gtcattttaa atagccaaaa
     1981 atcaaccagc caatttacat cgtgaatctg cgttcttggc tatctgtttt gggggggcca
     2041 agttggcttt actcatcctg gccgcttgcg gaacaaaaga cgttgttgat ctcaacagat
     2101 tgatttacct gttattgacc attcaattga accgcttaac cgccttttga gttccactac
     2161 tccccaaacc ccgtcttcgc cgcttgttgc tgtcagatat taatgctaat gtatgcatac
     2221 attagcatta atgagggatg atatgtgaga gacaaaacta gggaggccat gcggcttttt
     2281 ttaggcggca ggtgttacac ggctgaaaaa cttgaaaagg attatctggc tgaagttgct
     2341 aattacagtg acgaccgctg ggaagcaccg caacgcgccg cacgtctggc agctgccgta
     2401 aagcgttaca agacatcaga aatgctgcgc tttatttttg ctaccattgc
//
LOCUS       DAZFLD010000376         2450 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.378, whole genome shotgun sequence.
ACCESSION   DAZFLD010000376 DAZFLD010000000
VERSION     DAZFLD010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2450
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.378"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..497
                     /locus_tag="ACHKBL_004835"
     CDS             <1..497
                     /locus_tag="ACHKBL_004835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000502872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA];
                     GO_process: GO:0043170 - macromolecule metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043412 - macromolecule modification
                     [Evidence IEA];
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IbrB-like domain-containing protein"
                     /protein_id="HFO5821570.1"
                     /translation="DCVIWIKNNLIEANDYNPNVMAPSEKRLLTHSLLVDGFTQPVVT
                     LAQKGKYTVIDGFHRQMLGKSTTKLEKKLKGYLPVTCIKHDNNFKAYSIASTIRHNRA
                     RGKHQIDALSDIVRDLSRMGWDDLRIGSELGMDTDEVLRLKQISGLTELFEEENFSPA
                     WTIK"
     gene            complement(679..2424)
                     /locus_tag="ACHKBL_004836"
     CDS             complement(679..2424)
                     /locus_tag="ACHKBL_004836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001361889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HFO5821571.1"
                     /translation="MTAGEKRVASRLEAFMSNECLVWYDIPVGRKNRHPDFVIIDPEN
                     GLVFLEVKDWTVHTLHQVNHEQVILETNGILKSEINPLLQVRRYACDTVNALPADSRL
                     RQNDGQYKGRLNLAWAYGVVFTRITRQQLKALAGNDENAVERIFPSAQTICQDDMTQS
                     VLPDIFRQKIAGMFTMGFRTRVTPQVRDILRAHLFPEMTVRQHSQIKIMDIQQEILAR
                     NIGDGHRVIHGVAGSGKTMILLFRCLYLAETTPGKILVLCYNITLASYLRECIESRGL
                     KSRVTVFHFHSWCASMIKRHGIQVTAGGKDYPEKCFSALEDAVNSGTITDTGYDAVLV
                     DEGHDFESRWLALIARLFDNASRSLLLMYDDAQSIYRRERALNFSLASVGIQAQGRTS
                     VLPVNYRNTKRILHFAYAFSREYFEKHQNREIPFVQPQAGGEEGTEPEILRCASESDE
                     AVQVVGWLEKRYTLCGHWSDMAVLCPAEFSVKHLKEVMTQRGIPYATCFDSEGKKKYS
                     RRKDVVHLLTYQSSKGLEFPYVAVINASFVHSGAADESEVIPALYVAFTRATRELLVT
                     CYRENSISRHLEDFV"
BASE COUNT          735 a          614 c          499 g          602 t
ORIGIN      
        1 ttgattgtgt tatatggata aaaaacaatc taattgaagc aaacgactat aatcctaacg
       61 tcatggctcc ctccgaaaaa aggttattaa cacactccct gttggttgat ggattcactc
      121 aacctgttgt caccttagct caaaagggga aatacaccgt tattgacgga tttcacagac
      181 aaatgcttgg gaaaagcaca acaaaactag agaaaaaact aaaaggatat ttacctgtca
      241 catgcattaa gcatgataat aactttaaag cttactctat cgcctcgacc atcaggcata
      301 acagggccag aggaaagcac cagatagacg cgctctccga tatcgtccga gatctttccc
      361 gaatgggatg ggatgatctc cgaataggta gtgaactggg catggataca gatgaggtac
      421 tgagattaaa acaaataagt gggttaacag aactatttga ggaggaaaac tttagtccgg
      481 catggacaat aaagtaatca tttagggggt gactttatcc agtaattacc gactgatata
      541 tgatcacccc atgtaatgga cagctattga cagatcaaaa acatggggga tattaattca
      601 actctgaata ctcgctaaat acccatctta gattatcaaa aaacgaatga ttacatatca
      661 gcaataataa aacacaactt atacaaaatc ctcaagatga cgactgatac tgttttcacg
      721 ataacaggtc accagtaatt cccgtgtagc ccgggtgaac gcaacataaa gcgccggaat
      781 aacttcagac tcatccgcag cacctgagtg aacgaaagaa gcattgataa ccgcaacata
      841 cggaaactcc agtcccttac tgctctggta ggtcagtaaa tgcacaacat ccttgcgtcg
      901 cgaatatttc ttcttacctt cactgtcaaa gcaggtggca taaggtatcc cgcgttgtgt
      961 catgacctct ttcagatgtt tcactgaaaa ctctgcagga cacaaaacag ccatatcact
     1021 ccagtgaccg cacagtgtat agcgtttctc cagccagccc acaacctgca ctgcctcatc
     1081 actctcagaa gcacaacgca gaatttccgg ctctgtgccc tcttcaccgc ccgcctgtgg
     1141 ctgaacaaac ggtatttcac ggttctgatg cttctcaaaa tactcacggg agaatgcata
     1201 agcaaagtgc agaatgcgtt tcgtattacg gtaatttacc ggcaggacgg aagtacgtcc
     1261 ctgcgcctga atccccacac tggcaagcga aaagttcaga gcccgttccc gtctgtaaat
     1321 ggactgtgcg tcatcgtaca tcaggagcag tgaacgggac gcgttatcaa acaggcgggc
     1381 tatcagagcc agccagcgac tctcaaaatc atgcccctca tccacaagta cggcatcgta
     1441 tccagtatca gtaattgttc cgctgttcac ggcatcttcc agcgcactga agcatttctc
     1501 cgggtaatcc tttcctccag cagtcacctg aattccatgg cgctttatca ttgaggcgca
     1561 ccaactatga aaatggaata ccgtaacccg tgacttcagt cccctggact caatacattc
     1621 ccgcaggtag ctggccagag taatgttgta acatagcacc agaatcttac caggggtagt
     1681 ttcagccaag taaagacaac ggaacaaaag aatcattgtt tttccagagc cggctacccc
     1741 atgaataaca cgatgcccat caccaatatt tctggccaga atctcctgct gaatatccat
     1801 tattttgatt tgtgagtgct gtctgacggt catttcagga aacagatgag cacgcagaat
     1861 atctctaacc tgtggtgtca cccgggttct gaatcccatc gtaaacatcc cggcaatttt
     1921 ttggcggaat atatcaggta aaacagactg agtcatatca tcctgacaga ttgtctgcgc
     1981 tgaagggaaa atcctttcca cagcattttc atcattccca gccagagcct tcagttgctg
     2041 gcgggtaata cgggtaaaca caacaccata cgcccaggcc agattcagac gccccttata
     2101 ctgaccatca ttctgtcgca aacgtgagtc tgcaggtaaa gcatttacgg tatcacaggc
     2161 ataacgacga acctgtagca gcggatttat ttctgattta agaataccgt tagtttcaag
     2221 aatgacctgt tcgtgattta cctgatgcaa cgtgtgaacg gtccagtcct tgacttcaag
     2281 aaataccaga ccattctccg ggtcaataat gacaaaatca ggatgtcggt ttttccgtcc
     2341 caccggaata tcgtaccaga ccagacactc attactcata aatgcctcaa gacgggatgc
     2401 aacccgcttt tccccggcag tcatccgctc ctcggtaaca cctgatatat
//
LOCUS       DAZFLD010000377         2436 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.379, whole genome shotgun sequence.
ACCESSION   DAZFLD010000377 DAZFLD010000000
VERSION     DAZFLD010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2436
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.379"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..188
                     /locus_tag="ACHKBL_004837"
     CDS             <1..188
                     /locus_tag="ACHKBL_004837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000994797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HFO5821572.1"
                     /translation="VQPVQPVQPVQPVQHNIIGDLLRKSRKEARRAGRKQKRWEGACK
                     ALQELNKYRDLINELSE"
     gene            553..804
                     /locus_tag="ACHKBL_004838"
     CDS             553..804
                     /locus_tag="ACHKBL_004838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFO5821573.1"
                     /translation="MFVLIQRGQSFVDANNYPVEICKVTLTQVIYRRLDGRTRATSIG
                     AFNEEFERIEHNELHMIKAEIEKEKHIASLRKMRRTSIN"
     gene            850..1134
                     /locus_tag="ACHKBL_004839"
     CDS             850..1134
                     /locus_tag="ACHKBL_004839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family protein"
                     /protein_id="HFO5821574.1"
                     /translation="MRELVNQHNHGIQPVITPVVQINANEWVTLELLMAVTGLRKGTI
                     LRARDSAWMNGREYKQIAPDGTPKKNSECLYHLPTINTWIKNQPLPSQDV"
     gene            1187..>2436
                     /locus_tag="ACHKBL_004840"
     CDS             1187..>2436
                     /locus_tag="ACHKBL_004840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO5821575.1"
                     /translation="MANSAYPAGVENHGGKLRITFKYRGKRVRENLRVPDTPKNRKIA
                     GELRASVCFAIRTGTFDYAERFPDSPNLKLFGLVKKDITVGELAQKWLTLKAMEISSN
                     ALNRYQSVMKNMLPRLGPGRLASSITKEDLLFIRKDLLTGEKGSRKTSTSQKGRTVPT
                     VNYYMTTTAGMFSFAAENGYLEKNPFNSITPLRKSKPVPDPLTRDEFSRLIDACHHQQ
                     TKNLWTVAVFTGMRHGEIAALAWEDIDLKAGTITVRRNFTKIGDFTLPKTDAGTNRVI
                     HLLAPAIEALKNQAMLTRLGRQHQITVKLREYGRTILHECTFVFCPQIVRKNHKAGIN
                     YAVSSIGATWDSAIKRAGIRSRKAYQSRHTYACWALSAGANPTFIASQMGHSSASMVY
                     NVYGAWMPECSVTQVAMLNNILNSR"
BASE COUNT          754 a          558 c          574 g          550 t
ORIGIN      
        1 cagtgcagcc agtgcagcca gtgcagccag tgcagccagt gcagcacaac attataggtg
       61 acctgctacg taaatcacgg aaagaagcgc gtcgcgccgg gcggaaacag aaacgatggg
      121 agggtgcatg taaggcgctg caagaactga ataaataccg tgacttgatc aacgaattgt
      181 cagagtgagc gaatcacaga gagaatattt tgagaaacaa cccgcgtaca ttaatattgt
      241 ttttcctttc tctaatgacg ggcggtatcc tgttattcgc aggaacaaca ttatttattt
      301 ttgttgcccg gctaactgcg aggataatga catgaaaatc acatttgaaa gttacggttt
      361 aacagcaagt gtggttatat ccagctctat attcgaaagc cgcaaacatc agcatatcgt
      421 tgatgcagtg aaactcaaag ccccggaagt taccgtcaca acacatggac ttttcaggat
      481 acgaacgacg ttgactacaa cacacctctc ggcatgccgt gtttatgata tggcactcaa
      541 ggaatacaac cagtgttcgt cctgatccag cgcgggcagt ctttcgttga tgccaacaac
      601 tatccggtag aaatatgcaa ggtaactctg actcaggtga tctaccgaag gctcgacggc
      661 agaaccagag ccacttcaat tggtgcattt aatgaagaat ttgagcgaat cgagcacaac
      721 gaactgcata tgattaaagc ggaaattgag aaggaaaagc atattgccag ccttcgaaaa
      781 atgcgccgta catcaatcaa ctgacaaccg ccttcgggcg gttttttctt acacatgcag
      841 aggcaactta tgcgcgagtt agtaaaccaa cataaccatg gcattcagcc agtcatcaca
      901 cctgttgtac agataaatgc gaatgaatgg gtaacactgg agcttttaat ggctgtaaca
      961 ggcctgagaa aaggaacaat attacgcgcc agggacagtg cgtggatgaa cggcagagaa
     1021 tataaacaaa tcgcccccga cggaacgcca aagaaaaaca gcgaatgtct ctatcatctt
     1081 cctaccatca acacttggat caaaaaccaa cccttaccat ctcaggatgt ttaattcttg
     1141 tccataagag tataacctga gcgtgctctt ggacgcagga ggaacaatgg cgaattcagc
     1201 ctatccagcc ggcgttgaaa atcacggagg aaaactccga ataacgttta agtacagggg
     1261 taaacgagtg cgcgaaaatc ttcgcgtgcc cgatactccg aaaaacagaa agatcgctgg
     1321 tgagttaagg gcttctgtct gctttgcaat cagaacggga acgtttgatt atgccgagcg
     1381 attccctgac tcacctaacc tgaagctatt tggcctggta aaaaaagata tcaccgtcgg
     1441 tgaactggca cagaaatggc ttactctgaa agcaatggaa atcagtagta acgccttaaa
     1501 tcgttatcaa tcagtgatga aaaatatgct accgagactt ggtcctggca ggctggcgtc
     1561 atcgattaca aaagaagatc tgctgtttat caggaaagat ttactgaccg gggaaaaggg
     1621 aagcaggaaa accagcacgt cccaaaaagg aagaaccgta cccacagtga actattacat
     1681 gacaacaaca gccggaatgt tcagctttgc cgccgaaaac gggtatctgg agaaaaaccc
     1741 gtttaattca ataacaccgc tgaggaaatc aaaaccagtg ccggatccac tgaccagaga
     1801 tgagtttagc cgtctcattg atgcctgcca tcatcaacag accaaaaacc tctggacagt
     1861 ggctgttttt acagggatgc gacacggtga aattgccgca cttgcatggg aggatatcga
     1921 cctgaaagct ggcacgataa cagtgcgaag aaattttaca aaaataggtg attttacgct
     1981 accaaagact gacgcaggca ctaaccgggt tatacatctt ctggcaccag caattgaagc
     2041 acttaaaaac caggcgatgc ttactcgtct tggcaggcag catcagatca ctgttaaatt
     2101 acgcgagtac ggaagaacaa ttttgcacga gtgcactttt gttttctgtc cgcaaatcgt
     2161 tcgcaagaat cacaaggcgg gtattaacta cgcggtaagc tccatcggag cgacatggga
     2221 ttcagcaata aaaagagcgg gtatccgatc ccgtaaagcg tatcagtcac gccataccta
     2281 tgcgtgctgg gctttatctg ccggagcaaa cccgacattt attgcgtcac agatggggca
     2341 ctccagcgcc agcatggtct acaatgttta tggcgcatgg atgcctgagt gcagcgtgac
     2401 tcaggtagct atgttgaata atatccttaa ttcccg
//
LOCUS       DAZFLD010000378         2435 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.380, whole genome shotgun sequence.
ACCESSION   DAZFLD010000378 DAZFLD010000000
VERSION     DAZFLD010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2435
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.380"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            167..2308
                     /locus_tag="ACHKBL_004841"
     CDS             167..2308
                     /locus_tag="ACHKBL_004841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="HFO5821576.1"
                     /translation="MAMFTPSFSGLKGRALFSLLFAAPMIHATDSVTTKDGETITVTA
                     DANTATEATDGYQPLSTSTATLTDMPMLDIPQVVNTVSDQVLENQNATTLDEALYNVS
                     NVVQTNTLGGTQDAFVRRGFGANRDGSIMTNGLRTVLPRSFNAATERVEVLKGPASTL
                     YGILDPGGLINVVTKRPEKTFHGSVSATSSSFGGGTGQLDITGPIEGTQLAYRLTGEV
                     QDEDYWRNFGKERSTFIAPSLTWFGDNATVTMLYSHRDYKTPFDRGTIFDLTTKQPVN
                     VDRKIRFDEPFNITDGQSDLAQLNAEYHLNSQWTARFDYSYSQDKYSDNQARVTAYDA
                     TTGTLTRRVDATQGSTQRMHATRADLQGNVDIAGFYNEILGGVSYEYYDLLRTDMIRC
                     KKAKDFNIYNPVYGNTSKCTTVSASDSDQTIKQESYSAYAQDALYLTDNWIAVAGIRY
                     QYYTQYAGKGRPFNVNTDSRDEQWTPKLGLVYKLTPSVSLFANYSQTFMPQSSIASYI
                     GDLPPESSNAYEVGAKFELFDGITADIALFDIHKRNVLYTESVGDETIAKTAGRVRSR
                     GVEVDLAGALTENINIIASYGYTDAKVLEDPDYAGKPLPNVPRHTGSLFLTYDIHNMP
                     GNNTLTFGGGGHGVSRRSATNGADYYLPGYFVADAFAAYKMKLQYPVTLQLNVKNLFD
                     KTYYTSSIATNNLGNQIGDPREVQFTVKMEF"
BASE COUNT          673 a          572 c          564 g          626 t
ORIGIN      
        1 atgtagcaat tttttgttat ttctcattta ctactacatt actgcgtgat tgaatgattc
       61 tcaattacat atccgatggc gaaataacaa acggatagtg cttagtgagc aataagaaac
      121 aattttcact cgcgacaatc cgcgagtttt tgggataaca gactaaatgg ctatgttcac
      181 accttcattc tcaggactca aaggtcgggc gctcttttca ctgctttttg cggcaccgat
      241 gattcatgca acagactctg taacgaccaa agatggcgaa acaatcactg ttacagcaga
      301 tgcaaatacc gcaactgagg cgaccgatgg ttatcaacct ctgagcactt ccacggcgac
      361 attaaccgat atgccgatgc tggatatccc gcaggtggtc aatacggtta gcgatcaggt
      421 tctggaaaac cagaatgcga caacgctgga tgaggcgctt tataacgtca gtaacgtggt
      481 acagaccaat acattaggcg ggactcagga tgcctttgta cgccgtgggt ttggcgcaaa
      541 ccgggatggc tccatcatga ccaacggtct gcgaaccgta cttcctcgca gtttcaacgc
      601 cgccacagaa cgtgtggaag tgctaaaagg cccggcctcc acgctgtatg gcattctcga
      661 tcctggcgga ctgattaacg tcgtgaccaa gcgcccggaa aaaacattcc atggttcggt
      721 ttcagccacc tcctccagtt ttggtggcgg cactgggcaa cttgatatca caggtcccat
      781 tgaaggcact cagctggcgt atcgccttac cggggaagtg caggatgaag attactggcg
      841 aaacttcggt aaagagcgca gtacatttat tgccccgtca ctcacctggt ttggtgataa
      901 tgcaacagta accatgctct attcccatcg ggactataaa actccgttcg atcgtggaac
      961 gattttcgac cttacgacga aacagcccgt aaacgttgat cgaaaaatac gttttgacga
     1021 accgtttaat attacagatg gtcagtccga tctggcgcaa ctcaacgcag aatatcatct
     1081 caatagccag tggacagcgc gctttgatta cagctacagc caggataaat acagcgataa
     1141 tcaggcgcgt gttaccgcgt atgatgcaac gacaggaaca ctgacacggc gtgttgatgc
     1201 aactcaggga tctacccagc gtatgcatgc tactcgtgcg gatctgcaag ggaatgttga
     1261 tattgccgga ttctataatg agattctggg tggggtgtca tatgaatatt atgatcttct
     1321 gcgtacagat atgattcgct gtaaaaaagc taaagatttc aatatctaca accctgttta
     1381 tggtaatacc agcaaatgta caacggtttc ggcgtcggac agtgatcaga cgatcaaaca
     1441 ggagagctac tcagcgtatg cacaggacgc actctatctg accgataact ggattgccgt
     1501 cgccgggatc cgctatcagt attacacgca gtatgcgggt aaaggccgtc cttttaatgt
     1561 caatactgac agccgcgatg aacaatggac tcccaaactg gggttggtct acaaactgac
     1621 gccatcggta tccttatttg ccaattattc gcaaacattt atgccgcagt cgtcaattgc
     1681 cagctacatt ggcgatcttc caccggaatc atctaatgct tacgaagtcg gggcaaaatt
     1741 cgagctattc gatggtatca ccgcagatat tgcgctgttt gatatccata aacgtaatgt
     1801 gttgtatacc gaaagtgttg gtgatgaaac cattgccaaa acggcaggcc gtgttcgttc
     1861 aagaggggta gaagtcgacc ttgcgggagc attaactgaa aacattaata tcattgccag
     1921 ctacggctat accgatgcta aggttctgga agatcctgat tatgcaggta aaccattgcc
     1981 gaatgttcct cgtcataccg gttcgctatt cctgacctat gacattcata acatgcctgg
     2041 caataacaca ctgacgtttg gcggtggtgg acatggcgta agccgtcgtt cggcaaccaa
     2101 tggggctgac tattatctgc caggctattt tgttgccgat gccttcgccg catacaaaat
     2161 gaaattgcag tatccggtca cactgcaatt aaacgtcaaa aacctgtttg ataaaacgta
     2221 ttacacctct tccatcgcca caaataatct ggggaatcag attggcgatc cgcgtgaagt
     2281 gcaattcacg gtgaaaatgg aattttgatc aaaaaagccc ggtatgttta ccgggctgag
     2341 actgatgaca aactccaaat tgtctgatgc gctacgccca tcagatctac catagaaatt
     2401 caatatatta aattttcata tatttttagg ctgta
//
LOCUS       DAZFLD010000379         2417 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.381, whole genome shotgun sequence.
ACCESSION   DAZFLD010000379 DAZFLD010000000
VERSION     DAZFLD010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2417
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.381"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(45..283)
                     /locus_tag="ACHKBL_004842"
                     /pseudo
     CDS             complement(45..283)
                     /locus_tag="ACHKBL_004842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278287.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
     gene            complement(289..966)
                     /locus_tag="ACHKBL_004843"
     CDS             complement(289..966)
                     /locus_tag="ACHKBL_004843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001097314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5821577.1"
                     /translation="MNTRILFPLLFTVASFSASAGNWAVKNGWCQTMTEDGQALVMLK
                     NGTIGITGLMQGCPNGVQTLLGSRISINGNLIPTSQMCNQQTGFRAVEVEIGQAPEMV
                     KKAVHSIAERDVSVLQAFGVRMEFTRGDMLKVCPKFVTSLAGFSPKQTTTINKDSVLQ
                     AARQAYAREYDEETTETADFGSYEVKGNKVEFEVFNPEDRAYDKVTVTVGADGNATSA
                     SVEFIGK"
     gene            complement(1085..1969)
                     /locus_tag="ACHKBL_004844"
     CDS             complement(1085..1969)
                     /locus_tag="ACHKBL_004844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HFO5821578.1"
                     /translation="MSFSHSSLSAQVKSYLTFLPEEIRQKILEHLHGVIHYEPVIGIM
                     GKSGTGKSSLCNAIFQSRICTTHPLNGCTRQAHRLTLQIGERRMTLVDLPGIGETPQH
                     DQEYRELYRQLLPELDLIIWILRADERAYAADIAMHQFLLNEGADPSRFLFVLSHADR
                     VFPAEEWNDTEKCPSRHQELSLATVTVRVATLFPSSFPVLPVAAPVGWNLPAFVSLMI
                     HALPPQATSAVYSHIRGENRSEQARKHAQQTFGETIGKSFDVAVARFSFPAWMLQLLR
                     KARDRIIRLLVTLWDHLF"
     gene            2083..>2417
                     /locus_tag="ACHKBL_004845"
     CDS             2083..>2417
                     /locus_tag="ACHKBL_004845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5821579.1"
                     /translation="MTGLSEQAKELHMMMGRRKPPKIQAVLNTRYWKNTEKTIKRTVG
                     MNIHTLSQWCGEQYEKMFISKVAEVYMLHHTAAILRRKGVYVDVFKIPSKIMNPPEAR
                     ANCTGCPSS"
BASE COUNT          632 a          566 c          631 g          588 t
ORIGIN      
        1 cgtctcccgg agaagacgcg gcctgatgga tgaaatgaag acttttattg tctgagaagc
       61 ccttcactgg cgagccacac attaagctgt tttccggcgt cagactcaaa attccagcac
      121 cgccagcgta gctgcccttc ggcattgcgg ataaccagca ggaaatgcgt tccatggtca
      181 tcttcaataa gcacattgcg gtaaggtaag ctgttgtggc agccattgcc tgtttgcgtg
      241 agaaagggcc ttcgggcaat aaaacagggt gacagtccga cataccggct attttccgat
      301 aaattcaacg ctggcactgg tggcattacc gtcagcacca accgtgacgg tcactttgtc
      361 gtacgcacgg tcttcaggat tgaatacttc aaactcaacc ttattgcctt ttacttcgta
      421 agagccaaaa tcagcggttt ctgttgtttc ctcgtcatat tcccgggcgt atgcctgccg
      481 ggcagcctgc aggacggagt ctttattaat agtggtcgtc tgtttcgggg aaaaaccggc
      541 aagtgatgtg acaaatttcg gacagacctt cagcatatca ccgcgggtga attccattcg
      601 tacaccaaat gcctgtaaaa cggacacatc acgctctgct atggagtgaa cggctttttt
      661 gaccatttcc ggcgcctgtc cgatttccac ctcaacagcc ctgaatcccg tctgctgatt
      721 acacatttgt gatgtgggga tcaggttacc gttaatactg atacggctgc ccaggagcgt
      781 ctgtacgcca ttcgggcatc cctgcatcag gccggtaata ccaatcgtgc catttttcag
      841 cattaccagc gcctgaccat cttccgtcat ggtctggcac cagccgtttt tgacagccca
      901 gttaccggcg gaggcggaga aggatgccac agtgaacagc aggggaaaaa gaattctggt
      961 attcatgact gacctctgaa attaaagagt gtgttattga taatgacaga cggtatacgg
     1021 cagtggttac cgtaatccgt tgtcgtgtaa aggtatacgg ttgccacggg ggactccggg
     1081 gcgctcagaa caggtgatcc cacagtgtga ccagcaggcg gataatgcgg tcccgggctt
     1141 tacgcagaag ctgtaacatc caggccggaa aactgaaacg ggcaacggcg acgtcaaaac
     1201 ttttcccgat ggtttcacca aaagtctgtt gtgcgtgttt ccgggcctgt tcagagcggt
     1261 tttccccccg gatatgtgaa taaaccgcac tggttgcctg cggtggcagg gcgtggatca
     1321 ttagtgacac aaacgccgga agattccagc cgacaggcgc agcaacgggg agtaccggaa
     1381 atgatgacgg gaacagggtt gccacccgga ctgtaacagt tgccagtgag agttcctggt
     1441 gacgggacgg gcatttttct gtgtcgttcc attcttcagc aggaaacacg cgatcggcat
     1501 gactgagtac aaacagaaaa cgcgagggat ctgcaccttc attcagtaaa aactgatgca
     1561 tggcaatatc ggcagcatac gcacgttcat cagcccgcag gatccagata atcagatcca
     1621 gttccggcag taactgacgg taaagctccc ggtattcctg atcatgctgc ggtgtttcac
     1681 caatgccggg cagatcgacc agcgtcattc tgcgttcacc aatctgcagg gtgaggcgat
     1741 gagcctggcg ggtgcagccg ttcaggggat gcgtggtgca gatacgggac tgaaaaatgg
     1801 cattacacag actgcttttg ccggtgccgg atttacccat aatgccaatc acgggctcgt
     1861 aatgaataac accgtggaga tgttcaagga ttttctggcg aatttcttca ggaagaaacg
     1921 tcagatatga tttcacttgt gcagagaggg aagaatggct gaatgacata ccggtatcct
     1981 gttgatttca ctgaaccgga tcacagtaat ccggtttatc attcaggtaa tatgggcgta
     2041 taattatttc catttttaat ggaatatatt cggaggtaat aaatgacagg attatctgag
     2101 caggcaaaag aacttcatat gatgatgggg cggagaaaac cgccgaagat acaggctgtt
     2161 cttaacaccc gatactggaa aaacacagaa aaaacgataa aaagaactgt aggtatgaat
     2221 attcatacgc tcagtcagtg gtgtggagaa caatatgaga agatgtttat ttcaaaagtg
     2281 gcagaagtct atatgcttca ccatacggca gcaatattga ggcgtaaagg agtttatgtg
     2341 gatgttttta agattccctc aaaaataatg aacccgcctg aagcgagagc caattgtact
     2401 ggttgccctt cttctca
//
LOCUS       DAZFLD010000380         2407 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.382, whole genome shotgun sequence.
ACCESSION   DAZFLD010000380 DAZFLD010000000
VERSION     DAZFLD010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2407
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.382"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..250
                     /locus_tag="ACHKBL_004846"
     CDS             <1..250
                     /locus_tag="ACHKBL_004846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HFO5821580.1"
                     /translation="HDVPYLIAKKRNHPHADAIHVAGWVSLFTLHVIWPFLWIWATLY
                     RPERGWGMQNHDSSVVQLQQRIAGLEKQLADIKSSSAE"
     gene            256..1392
                     /locus_tag="ACHKBL_004847"
     CDS             256..1392
                     /locus_tag="ACHKBL_004847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFO5821581.1"
                     /translation="MDLLIILTYVAFAWAMFKIFKIPVNKWTIPTAALGGIFIVSGLI
                     LLMNYNHPYTFKAQKAVISIPVVPQVTGVVIEVTDKKNTLIKKGEVLFRLDPTRYQAR
                     VDRLMADIVTAEHKQRALGAELDEMAANTQQAKATRDKFAKEYQRYARGSQAKVNPFS
                     ERDIDVARQNYLAQEASVKSAAAEQKQIQSQLDSLVLGEHSQIASLKAQLAEAKYNLE
                     QTIVRAPSDGYVTQVLIRPGTYAASLPLRPVMVFIPDQKRQIVAQFRQNSLLRLAPGD
                     DAEVVFNALPGKVFSGKLAAISPAVPGGAYQSTGTLQTLNTAPGSDGVIATIELDEHT
                     DLSALPDGIYAQVAVYSDHFSHVSVMRKVLLRMTSWVHYLYLDH"
     gene            complement(1389..2363)
                     /locus_tag="ACHKBL_004848"
     CDS             complement(1389..2363)
                     /locus_tag="ACHKBL_004848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO5821582.1"
                     /translation="MTKLQLKYRELKIISVIAASENISHAATVLGIAQANVSKYLADF
                     ESKVGLKVFDRTTRQLMLTPFGTVLLPYINDMLDRNEQLNNFIADYKHEKRGRVTIYA
                     PTGIITYLSKHVIDKIKDIGDITLSLKTCNLERNAFYEGVEFPDDCDVLISYAQPKDE
                     SLVASFITQYAVTAYASQRYLEKHPISRPDELEHHSCILIDSMMIDDANIWRFNVAGS
                     KEVRDYRVKGNYVCDNTQSALELARNHLGIVFAPDKSVQSDLQDGTLVPCFQHPYEWW
                     LDLVAIFRKREYQPWRVQYVLDEMLREIRHQLAQSQQLRPEQAAESED"
BASE COUNT          603 a          569 c          605 g          630 t
ORIGIN      
        1 acatgatgtt ccctacctga ttgccaaaaa acgtaatcat cctcatgccg acgccattca
       61 tgttgctggt tgggtgagtc tttttacgtt acatgttatc tggccatttt tgtggatttg
      121 ggccacgctt taccgcccgg agcgtggatg gggaatgcaa aaccatgact cctccgttgt
      181 gcaactacaa cagcgtattg ccgggctgga gaaacagctc gctgacatca aatcctcttc
      241 tgccgagtaa tacttatgga tttactgatt attttaacct atgtggcttt cgcatgggca
      301 atgtttaaga tcttcaaaat tcctgtaaat aaatggacca ttcccacagc ggccctggga
      361 ggcatattta ttgtcagcgg tctaattctg ttaatgaact ataaccatcc gtataccttt
      421 aaagcgcaaa aagcggttat ttctattcct gttgtcccac aggtgacagg cgtggtgatc
      481 gaagtgacgg acaagaaaaa tacgctgatt aaaaaaggtg aggtgctatt tcgactggac
      541 ccgacgcgtt atcaggctcg ggtggatcgg ctgatggcgg atatcgttac cgcagaacat
      601 aaacagcggg cgttgggcgc agagttagat gagatggcgg cgaatactca gcaggcaaag
      661 gccacgcggg ataagttcgc taaagagtat cagcgttacg cacgtggcag ccaggcgaaa
      721 gtaaacccgt tttcagaacg cgatatcgat gtggcgcggc aaaattatct ggcgcaggaa
      781 gcctccgtaa aatcagcggc ggcggaacaa aaacagatcc agagccagct ggatagcctg
      841 gtgttgggtg aacattctca aatcgccagc ctgaaagcgc agctcgcgga agcaaaatat
      901 aaccttgagc agacgatagt gcgtgcgccg agcgatggtt atgtgaccca ggtgctgatt
      961 cgtccgggta cctatgccgc gtcgctgccg ctacgtccgg tgatggtgtt tatacccgat
     1021 cagaaacgac aaatcgtggc gcagttccgt cagaactcct tgctgcgcct ggctcccggc
     1081 gatgatgcgg aagtggtatt taacgctctg ccaggcaaag tgttcagcgg taagctggca
     1141 gccattagtc cagctgttcc cggtggagct tatcagtcga ccggcacctt acagacgtta
     1201 aacacagcgc cgggttcaga tggcgttatc gcgaccattg aactggatga gcatactgat
     1261 ttgagcgcat taccagacgg tatttacgcc caggtggcgg tctattctga tcatttcagc
     1321 catgtctcgg tgatgcgcaa agtactgtta cgcatgacca gctgggtgca ttacctttat
     1381 ctcgatcatt aatcttcgct ttccgccgct tgttcaggcc gcagttgctg cgactgagca
     1441 agctggtggc gaatttcacg cagcatttca tccagcacat actggacccg ccagggctgg
     1501 tattcccgtt tacggaagat agccaccaga tccagccacc attcatacgg gtgctgaaag
     1561 cagggcacca gcgtgccgtc ttgcaggtcg ctctgcacac ttttgtctgg cgcaaacaca
     1621 atccccaggt gatttcgtgc cagctccagc gcagattgcg tgttgtcgca aacgtaattt
     1681 cccttcacgc gataatcccg cacctcttta ctcccggcga cgttgaagcg ccagatattc
     1741 gcgtcgtcga tcatcatcga gtcgatcaaa atacaggagt gatgttcgag ttcgtcagga
     1801 cggctaatgg gatgtttctc aagatagcgc tggctggcat aagcggttac ggcatattgg
     1861 gtaataaaac tggcaaccag cgattcatct tttggttggg cgtagctgat taaaacatca
     1921 caatcatcgg gaaattcgac gccttcataa aaggcattac gctcaagatt gcaggttttt
     1981 agcgataagg tgatatcacc gatatcttta attttatcga ttacatgttt ggataaatag
     2041 gtaattatgc cggttggtgc atagatagtg acccgaccac gtttctcatg cttataatct
     2101 gcaataaaat tattaagctg ctcgtttctg tccagcatgt cgttgatata cggtaacagc
     2161 acggtgccaa aaggtgtgag catcagctgt cgggtggttc ggtcaaagac ttttaaaccc
     2221 acttttgatt caaaatcagc aagatatttg ctgacgttgg cctgtgcgat gccaagtaca
     2281 gtcgcggcat ggctgatatt ttcactggca gcgattaccg agataatttt taactcccgg
     2341 tacttaagtt gtaattttgt catcgcaaca gcccaattat atataatttt atatattact
     2401 atataaa
//
LOCUS       DAZFLD010000381         2399 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.383, whole genome shotgun sequence.
ACCESSION   DAZFLD010000381 DAZFLD010000000
VERSION     DAZFLD010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2399
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.383"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            32..298
                     /locus_tag="ACHKBL_004849"
     CDS             32..298
                     /locus_tag="ACHKBL_004849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFO5821583.1"
                     /translation="MTILDYIAANPGCSGSEIATALNTQTTAINAELRRLWRGGLVIR
                     TNRSTGGRARKTGGQASYHVNPMPFGCSNPLTHMFNQLLKEARA"
     gene            295..594
                     /locus_tag="ACHKBL_004850"
     CDS             295..594
                     /locus_tag="ACHKBL_004850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001274219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5821584.1"
                     /translation="MSTINHQKLRELAFALQRMATPQKLLAFRAMLSPSAVLALLDEL
                     EHARTTPLAIRLTLRHEIEDFCATLEAPGEPETPEAIQKELLQRIDKVFDFFLNH"
     gene            917..1144
                     /locus_tag="ACHKBL_004851"
     CDS             917..1144
                     /locus_tag="ACHKBL_004851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000013474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="derepression protein"
                     /protein_id="HFO5821585.1"
                     /translation="MANRKQQRARAERLHIRTEINRRLWRASRVAQIMHINMLHERTH
                     ALSNRYCAAVFSYLAEDMAELQSLINQQHHH"
     gene            1209..1625
                     /locus_tag="ACHKBL_004852"
     CDS             1209..1625
                     /locus_tag="ACHKBL_004852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5821586.1"
                     /translation="MKSVKGLSDIQDFFNWMWAVDPRLASRLNDWHDYYRRACKRAAR
                     RVTTYGDFSFTVDDRYRVDVKGDGDGFGLFHINGTDSTAISWSSSEMTLVLEMTNHSV
                     AVKGIYTAEDFIGEYQRLLVLYRAAFQAATAGECDA"
     gene            1622..>2399
                     /locus_tag="ACHKBL_004853"
     CDS             1622..>2399
                     /locus_tag="ACHKBL_004853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001279669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication endonuclease"
                     /protein_id="HFO5821587.1"
                     /translation="MSDHNAQLAYPWNAPLPVIDPETFGRAQPTALRQAIQSYINEDI
                     RTDARLDEETVAFLTTTDEGKRINSHLHHDEERRIRLEKLTRHKRENPPTLVSEAMAE
                     LRALPPFLAGPLIRDLARLSRKQESARREGLKNNSAYAADNFVRHGLRARLKRIARVN
                     ERFATPAFKATAARERLDELLMLPQLSRDEIQRLATLTATAFSTALERACDEVIEKTG
                     RGDDDLFTWLLTYQPLAHMALKLGITPPCWPSLEIRPDRRT"
BASE COUNT          579 a          736 c          627 g          457 t
ORIGIN      
        1 gaataaccgg aactgcaaag gagatctcac gatgaccatt ctggactaca tcgcagccaa
       61 tccggggtgc agcggaagtg aaatcgccac ggcactgaat acccagacca cagccattaa
      121 tgctgagtta cgccgactct ggcgcggcgg cttagtcatc agaacaaacc gcagcacagg
      181 tggtcgcgct cgcaaaacag gaggccaggc ttcttaccac gtaaacccga tgccgttcgg
      241 gtgtagcaat ccacttactc acatgtttaa ccagctactg aaggaagcca gagcatgagc
      301 accatcaacc accagaagct acgcgaactg gcatttgccc tgcaacgaat ggcaacgcct
      361 caaaaattac tggcgtttcg cgcaatgctc tcgccgtctg ctgtgctggc gctgctggat
      421 gagctggagc acgccagaac cacacctctc gccattcgcc tgacgctccg tcatgaaatc
      481 gaggatttct gcgcgacgtt ggaggcgcca ggcgaaccgg aaacgccgga agcaatacag
      541 aaagagctgc tgcaacgcat tgacaaggtt tttgattttt ttctgaacca ctaagaaacc
      601 agaacatgca cacacaaaaa aaccgcttgc cagcgttcgc ctggtcgggt tacagttttc
      661 ccgcacctca taaaacgggt gccgggattg caacccccgc cgactatgca cgcgcacaac
      721 cgcgccagcg gtttttttgt gcgtactgta tcgccacgtc tttttcgcat cgaaattatg
      781 gcggggcgta cggggccgac ttcggtcggg ccgggttcgt gcgtagccgg tggttgcaac
      841 cctgtacgtc tcgccaccca gaattgcaac ctctggatgg tgagttttca aaacttacta
      901 cgtacgaggc cacaccatgg caaaccgcaa acaacagcgc gcccgcgctg agcgtcttca
      961 catccgcact gaaatcaacc gcagactctg gcgcgcatca cgcgtcgcgc aaatcatgca
     1021 tatcaacatg ctgcatgagc gcacgcacgc gctatcaaac cgttactgcg ctgctgtctt
     1081 cagctatctc gccgaagata tggcagaact gcaaagcctc atcaaccagc aacaccacca
     1141 ctaaacaaca tcctgaagcc atcccgcccc gtcgggaggc ttccgcacgt ctgttaccgg
     1201 aggttctcat gaagagcgtt aagggacttt cagatattca ggactttttt aactggatgt
     1261 gggcagtgga tccccggctg gcttcccgcc tgaacgactg gcacgactat taccgccgcg
     1321 cctgtaaacg ggccgccaga cgcgtcacca catacgggga tttctccttc accgttgacg
     1381 accgttaccg cgtggacgtg aaaggtgatg gtgatggttt tggtttattt cacatcaacg
     1441 gaaccgacag caccgccatc agctggtcgt cgtcagagat gacgcttgtt ctggaaatga
     1501 caaaccacag cgtggccgtg aaaggtatat acaccgcaga agactttatc ggtgaatacc
     1561 agcgccttct cgtgctgtac cgggcagcgt ttcaggcggc aactgccggg gagtgcgacg
     1621 catgagcgac cataacgcac aactggcgta cccatggaac gccccgctgc cggttatcga
     1681 tcctgaaacg ttcggcagag cgcaaccaac cgcattacgc caggccattc agagctacat
     1741 caacgaagac attcgcactg acgccagact ggatgaagaa accgtcgcct tcctgacaac
     1801 cacagacgaa gggaagcgaa ttaattcgca tcttcatcac gacgaagagc gtcggatcag
     1861 actggaaaaa ctgacgcgac acaaacgcga aaatccaccc acgctggtat ctgaagccat
     1921 ggcagagctg cgcgccctgc cgccgtttct tgccggtccg ctcatccgtg acctggcccg
     1981 cctgagccgc aaacaggaga gcgcacgccg ggaagggctt aagaataaca gcgcatatgc
     2041 ggcggataac ttcgttcgtc acgggctgcg tgcccgcctg aaacgtatcg cccgtgtgaa
     2101 tgaacgcttt gccaccccgg cctttaaggc caccgccgcg cgcgaacggc tggatgaact
     2161 gcttatgctg ccgcagctga gccgtgacga aatccagcgc cttgccaccc tgaccgccac
     2221 cgcgttttcc accgcactgg agcgcgcatg tgatgaggtc atcgaaaaga ccggcagggg
     2281 tgacgatgac ctgttcacct ggttactgac ctaccagcca ctggcacaca tggcgttaaa
     2341 actgggtatc acgccaccct gctggccgtc gctggaaatc aggcccgacc gccgcaccg
//
LOCUS       DAZFLD010000382         2392 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.384, whole genome shotgun sequence.
ACCESSION   DAZFLD010000382 DAZFLD010000000
VERSION     DAZFLD010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2392
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.384"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            29..538
                     /gene="eco"
                     /locus_tag="ACHKBL_004854"
     CDS             29..538
                     /gene="eco"
                     /locus_tag="ACHKBL_004854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004867 - serine-type endopeptidase
                     inhibitor activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease inhibitor ecotin"
                     /protein_id="HFO5821588.1"
                     /translation="MKKESKKMKTILPAVLFAAFATTSAWAAESVQPLEKIAPYPQAE
                     KGMKRQVIQLTPQEDESTLKVELLIGQTLEVDCNLHRLGGKLESKTLEGWGYDYYVFD
                     KVSSPVSTMMACPDGKKEKKFVTAYLGDAGMLRYNSKLPIVVYTPDNVDVKYRVWKAE
                     EKIDNAVVR"
     gene            complement(688..2334)
                     /gene="mqo"
                     /locus_tag="ACHKBL_004855"
     CDS             complement(688..2334)
                     /gene="mqo"
                     /locus_tag="ACHKBL_004855"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008924 - L-malate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
                     /protein_id="HFO5821589.1"
                     /translation="MKKVTAMLFSMAVGLNAVSMAAKAKASEEQETDVLLIGGGIMSA
                     TLGTYLRELEPEWSMTMVERLEGVAQESSNGWNNAGTGHSALMELNYTPQNADGSISI
                     EKAVAINEAFQISRQFWAHQVERGVLRTPRSFINTVPHMSFVWGEDNVNFLRARYAAL
                     QQSSLFRGMRYSEDHAQIKEWAPLVMEGRDPQQKVAATRTEIGTDVNYGEITRQLIAS
                     LQKKSNFSLQLSSEVRALKRNDDNTWTVTVADLKNGTAQNIRAKFVFIGAGGAALKLL
                     QESGIPEAKDYAGFPVGGQFLVSENPDVVNHHLAKVYGKASVGAPPMSVPHIDTRVLD
                     GKRVVLFGPFATFSTKFLKNGSLWDLMSSTTTSNVMPMMHVGLDNFDLVKYLVSQVML
                     SEEDRFEALKEYYPQAKKEDWRLWQAGQRVQIIKRDAEKGGVLRLGTEVVSDQQGTIA
                     ALLGASPGASTAAPIMLDLLEKVFGDRVSSPQWQATLKAIVPSYGRKLNGDVAATERE
                     LQYTSEVLGLKYDKPQAADSTPKPQLKPQPVQKEVADIAL"
BASE COUNT          560 a          670 c          599 g          563 t
ORIGIN      
        1 cagcgacatc atcggtgaaa acctataaat gaagaaggaa agcaaaaaaa tgaagaccat
       61 tctacctgca gtattgtttg ccgctttcgc caccacttcc gcctgggcgg cagaaagcgt
      121 ccagccactg gaaaaaatcg caccttatcc acaagctgaa aaaggcatga agcgccaggt
      181 gattcagtta accccgcaag aagatgaatc taccctgaaa gtagaactgt taatcggcca
      241 gacgctggaa gtcgattgca atttgcatcg tctcggcggg aagctggaaa gcaaaacgct
      301 ggaaggctgg ggctatgatt attatgtctt tgataaagtc agttccccgg tttcgacgat
      361 gatggcctgc ccggatggca agaaagagaa gaaatttgtc accgcgtatc tgggcgatgc
      421 cggaatgctg cgttataaca gcaagctgcc gatcgtggtg tatacgccag acaatgtaga
      481 tgtgaagtac cgcgtctgga aggcggaaga gaaaattgac aacgcggtag ttcgctaaac
      541 tgctgtgaag tgctgcaacc gccaggtcgg ataaggcggt tacgccgcat ccgacatcta
      601 acgcccgagc cggttgcctg atgcgacgct gacgcgtctt atctgcatac gccacatccg
      661 gcataccatg ccggatgtgg cacatcatca caacgcaata tccgccactt ctttttgaac
      721 gggttgcggt ttcaactgcg gtttcggcgt actatctgct gcctgcggtt tgtcgtattt
      781 cagccccagc acttcgctgg tgtactgcaa ctcgcgttct gttgccgcta catcaccgtt
      841 cagcttgcgt ccataagacg gaacgatcgc tttcaacgta gcctgccatt gcgggctgga
      901 aacgcgatcg ccaaatactt tttccagcag atccaacata atcggcgcgg cggttgacgc
      961 ccctggcgat gcccccagca gcgcggcaat ggttccttgt tggtcgctga ccacttcggt
     1021 acccagacgc agtacgccac ctttctcggc atcacgcttg ataatctgca cgcgctgccc
     1081 cgcttgccac aaacgccagt cctctttttt cgcctgcgga tagtactctt tcaacgcttc
     1141 aaaacgatcc tcttcactca acatcacctg actcaccaga tatttcacca gatcgaaatt
     1201 atccagcccg acgtgcatca tcggcatcac gttagaggtg gtggtggaac tcatcagatc
     1261 ccacaatgaa ccgtttttga ggaatttggt tgagaaggtg gcaaatggcc caaacagcac
     1321 gacgcgttta ccgtccagaa cacgggtatc gatatgcgga accgacatcg gtggtgcgcc
     1381 aacggatgct ttaccgtaaa ccttcgccag atggtgatta accacgtccg ggttttccga
     1441 aacaaggaac tgtccgccca ccgggaaacc ggcgtagtct ttcgcttccg gaatccccga
     1501 ttcctgtaac agcttcagcg ccgcaccgcc cgcgccgata aagacaaatt tggcgcggat
     1561 gttctgtgca gtgccatttt tcagatcggc aacagtgacg gtccaggtgt tatcgtcatt
     1621 acgctttagg gcgcggactt cgctgctgag ttgcagcgaa aaattagatt tcttctgcaa
     1681 ggaagcaatt aactggcggg tgatctcgcc atagttcaca tcagtaccaa tttccgtacg
     1741 cgtggctgcc actttctgtt gcggatcgcg cccttccatc actaacggtg cccactcttt
     1801 gatctgcgcg tgatcttcag agtaacgcat accgcgaaac agcgagcttt gttgcaacgc
     1861 ggcgtaacgg gcgcgcagga aattgacgtt atcctcgccc cagacaaagc tcatatgcgg
     1921 aacggtattg ataaatgaac gcggagtacg cagcacgccg cgctcgacct ggtgcgccca
     1981 gaactggcgc gaaatctgaa atgcttcgtt aatggcgact gctttttcaa tactgatgct
     2041 gccatcggcg ttttgcggag tgtaattgag ttccatcagt gcagaatgcc cggttccggc
     2101 gttgttccag ccgttcgaac tctcctgcgc gacaccctcc aggcgctcca ccatggtcat
     2161 cgaccattca ggctccagct cgcgtaaata ggtccccaac gtggcgctca taatgccgcc
     2221 gccaatcaac agtacatcag tttcctgctc ctcggatgct ttcgcttttg ccgccatcga
     2281 aacggcatta agccccacgg ccatcgagaa gagcatggca gtcacttttt tcatcttgtt
     2341 aatgccttac ttttagtcgc tttattgcag gtgagatttg cgcggcatca ac
//
LOCUS       DAZFLD010000383         2385 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.385, whole genome shotgun sequence.
ACCESSION   DAZFLD010000383 DAZFLD010000000
VERSION     DAZFLD010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2385
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.385"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1979
                     /gene="ireA"
                     /locus_tag="ACHKBL_004856"
     CDS             <1..1979
                     /gene="ireA"
                     /locus_tag="ACHKBL_004856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001534707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor IreA"
                     /protein_id="HFO5821590.1"
                     /translation="SSDKKEDTLVVTASGFTQQLRNAPASVSVITSEQLQKKPVSDLV
                     DAVKDVEGISITGGNEKPDISIRGLSGDYTLILVDGRRQSGRESRPNGSGGFEAGFIP
                     PVEAIERIEVIRGPMSSLYGSDAIGGVINIITKPVNNQTWDGVLGLGGIIQEHGKFGN
                     STTNDFYLSGPLVKDKLGLQLYGGMNYRKEDSISQGTPAKDNKNITATLQFTPTESQK
                     FVFEYGKNNQEHTLTPGDSLDAWTMRGNLKQPNSKRETHNSRSHWVAAWNAQGEILHP
                     EIAVYQEKVIREVKSGKKDKYNHWDLNYESRKPEITNTIIDAKVTAFLPENVLTIGGQ
                     FQHAELRDDSATGKKTTETQSVSVKQKAVFIENEYAATDSLALTGGLRLDNHEIYGSY
                     WNPRLYAVYNLTDNLTLKGGIAKAFRAPSIREVSPGFGTLTQGGASIMYGNRDLKPET
                     SVTEEIGIIYNNDSGFSASATLFNTDFKNKLTSYDIGTKDPVTGLNTFIYDNVGEANI
                     RGVELATQIPVYDKWRVSANYTFTDSRRESDDESLNGKSLKGEPLERTPRHAANAKLE
                     WDYTQDITFYSSLNYTGKQIWAAQRNGAKVPRVRNGFTSMDIGMNYQFLPGTLINFAV
                     LNVTDRKSEDIDTIDGNWQVDEGRRYWANVRISF"
BASE COUNT          750 a          462 c          525 g          648 t
ORIGIN      
        1 ccagttctga taaaaaagaa gatacgcttg ttgttactgc ctccggcttc actcagcaac
       61 tcagaaatgc accggccagt gtttcagtca ttacttcaga gcaactgcaa aaaaaaccgg
      121 tttcagacct ggtcgatgcg gtaaaagatg ttgaagggat tagtatcacc ggtgggaatg
      181 aaaaaccgga tatcagtata cgtgggttaa gtggcgatta tacattgatt ctggtcgatg
      241 gacgacgtca gagtggtcgg gaatccagac caaacggcag cggcggtttt gaagccggat
      301 ttatccctcc tgtggaagca attgagcgta ttgaagttat acgtggccct atgtcttccc
      361 tgtatggttc cgatgctatc ggaggagtta ttaatatcat aaccaaacct gttaataacc
      421 agacatggga tggcgtactt gggcttgggg gaattattca ggaacatggg aaatttggta
      481 actcaaccac gaatgacttc tacctgtcag gcccattggt taaggataaa cttggtcttc
      541 agttatatgg aggaatgaac tatcgcaagg aagatagtat ctctcaggga acaccagcaa
      601 aagataataa gaatataacg gcaacactcc agtttactcc gactgaaagc cagaagtttg
      661 tttttgaata cggaaaaaat aaccaggaac atacattaac acctggtgat tctcttgatg
      721 cctggactat gcggggaaat cttaaacaac caaacagtaa aagagaaaca cataactcac
      781 gtagtcactg ggtagcagca tggaatgccc agggcgaaat actgcatcct gaaattgctg
      841 tttatcagga gaaagttatt cgtgaggtta aatctggtaa aaaagataaa tataatcatt
      901 gggatcttaa ttacgagtca agaaaaccgg aaataaccaa cacgatcata gatgcaaaag
      961 tgacggcatt tctgccggaa aatgtgctga ccatcggagg tcaatttcag catgcagaac
     1021 tccgtgatga ctcagccaca ggtaaaaaaa cgacagaaac acagtctgtt tcagttaaac
     1081 agaaagctgt ttttatagaa aatgaatatg ctgcaacgga ttctctcgcc ctgactggag
     1141 ggctgcgcct cgataaccat gaaatctatg gcagttactg gaatccaaga ttgtatgctg
     1201 tttataacct gaccgataat ctcactctca aaggagggat cgcaaaagca tttcgggccc
     1261 cttcgattcg tgaggtcagt cctggatttg gaacactgac gcagggtggt gcctctatta
     1321 tgtatggaaa cagggacctg aaaccggaga ccagtgtaac cgaagagatc ggtattattt
     1381 ataataatga cagtggtttt tcagcaagcg cgactctgtt taatacagat tttaaaaata
     1441 agctgaccag ttacgatata ggcacaaaag atccagtcac tgggctaaac acttttattt
     1501 atgataatgt gggtgaggca aatatcagag gtgtggagct tgcaactcag attcctgtgt
     1561 atgataaatg gcgtgtatct gcaaactata cattcactga ctcccgtcga gaaagcgatg
     1621 acgaaagtct caatggcaag tcgctgaaag gggaacctct ggaaagaact cccagacatg
     1681 cagccaatgc aaaactggaa tgggattaca ctcaggatat tacattttat tcatctctga
     1741 attatacagg aaaacaaatc tgggcagcac aaagaaatgg tgccaaggtt ccccgtgttc
     1801 gtaatggatt cacttctatg gatattggta tgaattacca gttcctgcca ggcacgctga
     1861 ttaattttgc cgtacttaac gtcacagaca gaaagagcga agatatcgat accattgatg
     1921 gtaactggca ggtcgatgaa ggacgtcgtt actgggctaa tgtaagaata tccttctgag
     1981 agaatataga taacctgagg ccgtgcgcct caggtaccag tatagtaatc agtaacgtca
     2041 gcttccaggt tgtaatgctt ttaattttga taaaaataaa cttaataaat tacatttgtt
     2101 aaatattgat aattttaatt tggttttatt actccagtta tactcacact ctatctttac
     2161 acaattcatc accattgcac ctccatagcc tgttgattta aaagatttaa tttcctgtga
     2221 aacaatcagg cctgtatgaa cctgattata ccacataatc ttttaagtta tttaaattta
     2281 actctcgggc ttttctgtca gtaaaatcag aaaaattgca ggatttcgtt tttttgctgc
     2341 gaagtgaaat gtatctcatt tttactgcct tcgccagtgc gtcag
//
LOCUS       DAZFLD010000384         2377 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.386, whole genome shotgun sequence.
ACCESSION   DAZFLD010000384 DAZFLD010000000
VERSION     DAZFLD010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2377
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.386"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..353)
                     /locus_tag="ACHKBL_004857"
     CDS             complement(<1..353)
                     /locus_tag="ACHKBL_004857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFO5821591.1"
                     /translation="MTTFSHSSTTPSVSVTTASGSDQPQLVATIVPDEQRISFWPQHF
                     GLIPQWVTLEPRIFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDGENWVLFNA
                     MNGNRAEMSPEAAGIA"
     gene            complement(444..1262)
                     /locus_tag="ACHKBL_004858"
     CDS             complement(444..1262)
                     /locus_tag="ACHKBL_004858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HFO5821592.1"
                     /translation="MRLASRFGYANQIRRDRPLTREELMQVVPSVFGEEKHTSRSENY
                     TWIPTITVLESLQREGFQPFFACQTRVRDPGRREYTKHMLRLRRAGQITGQHVPEIIL
                     LNSHDGTSSYQMLPGYFRFVCQNGCVCGQSLGEVRVPHRGNVVDRVIEGAYEVVGVFD
                     RIEEKRDAMQSLVLPPPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLWS
                     AYQTIQENMLKGGISGRSAKGKRIHTRAIHSIDTDIKLNRALWVMAETLLESMR"
     gene            complement(1589..>2377)
                     /locus_tag="ACHKBL_004859"
     CDS             complement(1589..>2377)
                     /locus_tag="ACHKBL_004859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HFO5821593.1"
                     /translation="LEGDLLRTEVAGMSLTTGVYGAAGHSSVDVKDDDGSRAGTVRDD
                     AGSLGGYMNLTHTSSGLWADIVAQGTRHSMKASSGNNDFRARGWGWLGSLETGLPFSI
                     TDNLMLEPRLQYTWQGLSLDDGKDNAGYVKFGHGSAQHVRAGFRLGSHNDMTFGEGTS
                     SRAPLRDSAKHSVRELPVNWWVQPSVIRTFSSRGDMRVGTSTAGSGMTFSPSQNGTSL
                     DLQAGLEARVRENITLGVQAGYAHSVSGSSAEGYNGQATLNVTF"
BASE COUNT          522 a          662 c          671 g          522 t
ORIGIN      
        1 gcaatgccgg cggcttccgg gctcatttca gcgcggttac cgttcatggc attgaacagt
       61 acccagtttt cgccatcatc gtcatccggt tcgggggcca taaatgcgcc accgttgttc
      121 agtgtgtaca gattccagat accaccgcag tagtcttcgc aaagacggtc catccagccg
      181 aagatacggg gctccagggt cacccactgt ggaatgaggc caaaatgctg cggccagaag
      241 ctgatgcgct gttcatcagg gactatggtg gcaaccagct gaggctggtc actccctgat
      301 gcagttgtta cggaaacaga aggtgtggtg gagctatgcg aaaaggttgt catgaaatga
      361 ttccttataa aaagcaaatg aatgaagaag actcgggggc agggacagac gtgagtcagg
      421 acggcgcttt caggtaaacg gtatcagcgc atgctctcca gcagcgtttc cgccatcacc
      481 cacaatgcgc gattgagctt gatgtcggtg tcgatactgt gaatggcacg ggtatggata
      541 cgttttcctt ttgcactacg gccggaaatc ccgcctttca gcatattctc ctggatggtc
      601 tgatatgcgc tccacaggtc cttaccgtaa tcctcccggc gtcgtggtgt cagaatgtcg
      661 gcagtggtga cgggttgatg ttcatcacca taacggtaag tcagcgccgc ctgtgccagt
      721 gcctgacgtg ccggtggcgg cagaaccaga gactgcatgg catcacgctt ttcctcaatc
      781 cggtcaaaaa cgcccactac ctcgtaagcc ccttcaatga ctctgtccac cacatttccc
      841 cggtggggca cacggacttc ccccagagac tgaccgcaga cgcagccgtt ctggcagacg
      901 aatctgaagt aacccggcag catctggtag ctggaggtac cgtcatggga gttgagcaga
      961 ataatttcag gcacatgctg accggttatc tgtccggccc gccgcagacg cagcatgtgt
     1021 ttggtatatt cccggcggcc cgggtcacgt acgcgggtct ggcaggcgaa gaatggctga
     1081 aagccttccc gctgcaggct ttccagcacg gtgatggtgg gaatccaggt atagttctcg
     1141 cttctggatg tgtgtttctc ttccccgaat acgctgggga cgacctgcat cagttcttca
     1201 cgagttaagg ggcggtcacg gcgtatctgg ttcgcataac caaaacgact ggctaatcgc
     1261 atgatttatt ccttatgaat gagttagcgg tataaatgaa aaagccgcgt ctcccggaga
     1321 agacgcggcc tgatggggga aatgaatgac gtttattgtc tgagaagccc ttcactggcg
     1381 agctgcgtat ttttatggaa ggagatcacc caataacccc atcgggatcg ggtaataacc
     1441 ggtcagactg tgatcgattc agatgccatt tggcagcaaa agagccttcg gcaataaacg
     1501 tgctgtacaa cccggcatat ctgttgctcc ctgaaagtaa aaagccccgg tcatgatgac
     1561 cggggcctga aggggagtga cctgattatc agaaagtcac attcagcgtg gcctgaccgt
     1621 tatagccttc agcgctgctg ccgctgacgc tgtgggcata accggcctga acgcccaggg
     1681 tgatattttc ccggacacgg gcttccagtc cggcctgcag gtccagtgat gtgccattct
     1741 gtgagggaga gaacgtcatc ccgctgcctg cagtggaagt ccccacacgc atatctcccc
     1801 gggagctgaa ggtgcggata acggaaggct gtacccacca gttcaccggc agttcacgca
     1861 cactgtgttt tgcactgtca cgcagggggg cacgggatga ggtgccttca ccaaaggtca
     1921 tatcgttgtg gctgcccaga cggaaaccgg cacgcacatg ctgtgcactg ccatgcccga
     1981 acttcacata accggcgttg tccttaccgt catccagcga aagcccctgc caggtgtact
     2041 gcagccgtgg ctccagcatc aggttgtcag tgatactgaa gggcagaccg gtttccagtg
     2101 agcccagcca gccccagccc cgtgcgcgga agtcgttatt gcccgatgac gctttcatac
     2161 tgtggcgggt tccctgtgcc acaatgtcag cccacaggcc ggaggaggtg tgtgtcaggt
     2221 tcatatatcc gcccaggctg ccggcatcat cccggaccgt gccggcgcgg gagccgtcat
     2281 catccttaac atcaacggaa gaatggcctg cagcaccata cactcctgtc gtcagtgaca
     2341 taccggcgac ctctgttctg agcaggtcac cctccag
//
LOCUS       DAZFLD010000385         2370 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.387, whole genome shotgun sequence.
ACCESSION   DAZFLD010000385 DAZFLD010000000
VERSION     DAZFLD010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2370
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.387"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            63..1151
                     /gene="tssG"
                     /locus_tag="ACHKBL_004860"
     CDS             63..1151
                     /gene="tssG"
                     /locus_tag="ACHKBL_004860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004160261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HFO5821594.1"
                     /translation="MERESQPVRTGLTQALALNNDIWRASFYRFCQLLEQENPDGPKL
                     GTTSHPGDDPVRFRPWPGMGFPVSTLKAVEIDEDRPTLPPTIRTTFLGMYGVDSPLPT
                     SWLDDITQRREGHEALTSFLDIFSHRITTQYYRIWRKYAYPATFEEGGRDATSQCLLG
                     LVGLGIPGTAEQVATPVSRFLALLGAMRLPTRNAEGIRALVSLLAPDTRALITEPDPV
                     KVHIGNRSGLGAGNRIRLSQRAPLGKTTGEACSRLLMTLETADPNEAEGWLPGGVLHT
                     DLLVLLRVYLGYRSDVRLRLTVPVRLLPEPRLGKGRPVQLGRTGLLGLKAGKLSNGRE
                     SLTVSLGCYEGLKCAALPPAEDGHYRFE"
     gene            1187..1711
                     /gene="tssJ"
                     /locus_tag="ACHKBL_004861"
     CDS             1187..1711
                     /gene="tssJ"
                     /locus_tag="ACHKBL_004861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000259061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system lipoprotein TssJ"
                     /protein_id="HFO5821595.1"
                     /translation="MVTVIRWWVPMFIACGLLAGCGLTQTVTDGTISITKSIFYKKIK
                     TLHLDFTPRTAINTDGAQTPLATMVRVYQLRDRKAVDVAGYQMLLRNADTVLKDDVLA
                     SKELLVMPGGSVTLNMPMDEDAQFVAVVGLFNRPDQKDNRWRLVLTRDDLNPDKPRTI
                     ELGDGWLSLVPVKE"
     gene            1716..>2370
                     /locus_tag="ACHKBL_004862"
     CDS             1716..>2370
                     /locus_tag="ACHKBL_004862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system Vgr family protein"
                     /protein_id="HFO5821596.1"
                     /translation="MDNRSAFFDGQTRYFLEINGSAVKPDVLRFRGREALSEPFRWDI
                     GFTSRNANIPPEEVLMKYASLRMRNGKNVHGIVTRLEWLSTSKDQSHYRLTLSSRLAL
                     LEYTRQCAVFQNQSVPEVVEQVLRKHGLEGADFEFRLERAYPSREIITQWQETDLQFI
                     QRILSEVGIFWRTEMDDVRGLDTYIFADSQLNYQFDVCLPYSEPSGLFDGAAESVWDV
                     "
BASE COUNT          502 a          643 c          708 g          517 t
ORIGIN      
        1 gctgatgttc accttttcaa ccagctcaca ctggtactgc aacccacagg gaaacgactg
       61 agatggaaag agaatcacag ccggtacgta ccgggctgac gcaggccctg gcccttaaca
      121 acgatatctg gcgggccagt ttttatcgtt tctgccagct gctggagcag gaaaatccgg
      181 acgggccaaa actgggcacg accagccatc cgggcgatga tccggtgcga ttcagaccct
      241 ggcccgggat gggattcccg gtcagcaccc tgaaagcggt ggagatcgac gaagaccgtc
      301 cgacattacc cccaacaatc cgcacgacat ttctcggaat gtatggggtg gactcacctc
      361 tgccgacgtc ctggctggat gatattacgc agcgccgtga aggacatgag gcactcacct
      421 catttctgga tattttcagc catcgcatca ccacgcagta ctaccggatc tggcgaaaat
      481 atgcgtaccc ggccactttt gaggaggggg ggcgcgatgc cacatcgcag tgtctgctgg
      541 ggctggtcgg gctcggtata cccggcaccg ctgaacaggt ggcaacgccg gtttcccgtt
      601 tcctggcctt actgggtgcc atgcgattac ccacccgcaa tgctgaaggc atccgggctc
      661 tggtgagtct gctggcaccg gatacccgcg cacttatcac cgaacccgat ccggttaagg
      721 tccatatcgg taaccgcagt ggactgggtg ccggaaaccg catccgtctt tcgcagcggg
      781 cgccgctggg taaaacgaca ggggaagcat gcagccggct cctgatgaca ctggaaacag
      841 cagatccgaa cgaagcggaa ggctggttgc ccggtggtgt cctccatacc gatttgctgg
      901 tcctgctgcg tgtttatctc ggctaccgca gtgacgtccg gctgcgactc actgtccccg
      961 tgcggttgtt gcctgagccc cgcctgggca aaggcaggcc tgttcagctg ggccgtaccg
     1021 gtttgctggg gctgaaagcg ggcaagctca gcaatggccg ggagagtctg acggtcagtc
     1081 tgggctgcta cgaagggttg aaatgtgctg cgctaccgcc tgctgaagac ggacattatc
     1141 gcttcgaata atcacctgtt ctctctctca ataaaggagc gcctgaatgg taacagtaat
     1201 ccgctggtgg gtgccgatgt ttattgcctg tggcctgctc gcaggctgcg gtctgacgca
     1261 gaccgtcacg gacggcacga tcagcatcac gaaatccatc ttctacaaaa aaatcaaaac
     1321 gctgcatctg gattttaccc cacgcacggc cattaacact gacggggcac agacgccact
     1381 ggcaaccatg gtgcgggttt accagctcag ggatcgtaaa gccgttgatg tcgccggtta
     1441 ccagatgctg ctgcgtaatg ccgatacggt acttaaggac gacgtgctgg cctcaaaaga
     1501 gctgctggtg atgcccggcg gcagcgtgac gctcaatatg cccatggatg aagatgcgca
     1561 gtttgtggct gtggtgggat tgtttaaccg gccggatcag aaggacaatc gctggcgcct
     1621 ggtactgacc cgtgatgacc tcaatccgga taagccccgc accattgaac tgggtgatgg
     1681 ctggctcagc cttgtgccgg taaaggagtg acgtgatgga taaccggtca gcgttttttg
     1741 acgggcagac ccgttatttt ctggaaatta atggcagtgc agtaaagcct gatgttttac
     1801 gttttcgtgg cagggaagca ctgagtgagc cgttccgatg ggacatcggg ttcacctccc
     1861 ggaatgcgaa cattcctccg gaagaggtgc tgatgaaata tgcgtcgttg cggatgcgta
     1921 acggcaaaaa tgtccatggc attgtgaccc gtctggaatg gctgtccaca tcgaaagacc
     1981 agtcccacta ccggcttaca ctcagctccc gtctggcact gctggagtac acccggcagt
     2041 gtgccgtatt tcagaaccag tccgtccctg aggtggtgga gcaggtgctg cgcaaacacg
     2101 ggctggaggg cgctgatttt gaattcagac tggaacgcgc ttacccgtcg cgtgaaatta
     2161 tcacccagtg gcaggaaacg gatttgcagt tcattcagcg cattctgtct gaggtgggga
     2221 ttttctggcg cacggagatg gatgatgtgc gcggactgga tacgtacatt tttgccgaca
     2281 gccagcttaa ctaccagttt gatgtgtgtc tgccgtacag cgagccgtcg ggcctttttg
     2341 acggtgctgc agaatcggtc tgggatgtac
//
LOCUS       DAZFLD010000386         2365 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.388, whole genome shotgun sequence.
ACCESSION   DAZFLD010000386 DAZFLD010000000
VERSION     DAZFLD010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2365
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.388"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            76..1518
                     /locus_tag="ACHKBL_004863"
     CDS             76..1518
                     /locus_tag="ACHKBL_004863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004100781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFO5821597.1"
                     /translation="MQVDWLIKNVTVIDGSGGPEFRGDVAITGDRIVDIAPALNVTAQ
                     QVIDGEGRVLAPGFIDVHTHDDINVIRIPEYLPKISQGITTVIVGNCGISAASAKMKG
                     EVPDPMNLLGEAEHFIYPTVESYAQAVEAARPSLNVGTLIGHTALRNNHMDDLFRPAT
                     VDEIAAMRADLRLALSQGALGLSSGLAYATAFQATTEEVMALAEELAGEKGVYTTHLR
                     SEFEPILDALDEAFRIGRHGKVPVVVSHHKCAGAKNWGRTKETLAFFDQMRQHQEIGC
                     DVYPYSASSSTLDLKQVTDEFDIVITWSQTHPQQAGKTLAQIAIDWQMSMLEAAKLLM
                     PAGAIYYNMDERDVRRVLSYPVSMIGSDGLPNDPMPHPRLWGAFPRVLGHYCRDEGLF
                     PLTTAIHKMTGLSASRFRLPQRGLVKVGYFADLVLFDPQTIRDVASFSDPKRPADGIE
                     AVMVNGVMSYGPDKHITGRAGRFLRRQTSH"
     gene            1528..1917
                     /locus_tag="ACHKBL_004864"
     CDS             1528..1917
                     /locus_tag="ACHKBL_004864"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000023755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFO5821598.1"
                     /translation="MSIKRYGTEGGTGTGGQHLPFARAVEAGGWLYVSGQTPMKNGEV
                     VEGGIVEQSRLAIQNCVDIMTEAGYSLADVVHVKVILTDARYFQSFNKVFREFFGENP
                     PARICCVADLVVDCKVEVDVTCYNATR"
     gene            2020..>2365
                     /locus_tag="ACHKBL_004865"
     CDS             2020..>2365
                     /locus_tag="ACHKBL_004865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004108532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HFO5821599.1"
                     /translation="MNSHIFLVGFIIYAIAMIWLGWFVSRNQKSGEDFLLGGRSLPLF
                     LTLGSTVATMVGTGSSMGAVGFGYSNGWAGMLYGVGGAVGILLVAWLFAPVRKLRFMT
                     MSEELSYYTGGSH"
BASE COUNT          535 a          552 c          676 g          602 t
ORIGIN      
        1 tttgcgtagt tttccactgt ttttcgtcct tgtaaatggt taacttgtca ccattgcaga
       61 aatggagtga cgaacatgca ggttgactgg ttaatcaaaa atgtgaccgt tatcgacggt
      121 agcggcggcc ctgaatttcg cggtgacgtg gcaataacag gtgatcggat tgtcgatatt
      181 gcccctgcgc ttaacgttac ggcgcagcag gtcattgacg gggaagggcg ggtgctggca
      241 cccgggttta tcgacgttca tacccatgac gatatcaacg ttattcgcat cccggaatat
      301 ctgccaaaga tcagccaggg gatcactacg gtgattgtcg gtaactgcgg gattagtgcg
      361 gcgtcggcga aaatgaaagg tgaagttcct gacccgatga atctgttggg ggaagcagag
      421 cactttattt atcctaccgt tgaaagctat gcccaggcag tggaagcagc caggccgtca
      481 ctgaacgttg gcacgctgat tggtcatacg gcgctgcgta ataaccatat ggacgacctg
      541 tttcgcccgg cgacggtgga tgaaattgcc gctatgcgtg ccgacttacg tctggcgttg
      601 agtcaggggg cgctggggtt aagttccggg ctggcctatg caacggcatt tcaggcgact
      661 accgaagaag tcatggcgct ggcggaagaa ttagccggtg agaagggcgt ttataccacc
      721 catctgcgtt ctgaatttga accgattctc gatgccctgg atgaggcgtt tcgtattggg
      781 cgtcatggca aagtgcctgt cgtggtttcc caccataaat gcgccggggc gaaaaactgg
      841 gggcggacaa aagaaacgtt ggcctttttt gaccaaatgc gtcaacacca ggaaattggc
      901 tgcgatgtct acccttactc agccagctct tcaacgctgg atctcaaaca agtcaccgac
      961 gaattcgata ttgtgatcac ctggtcacaa acacatccgc agcaggccgg gaaaacactg
     1021 gcgcaaatcg ccatcgactg gcagatgagt atgctggagg ccgcgaagct gctaatgcct
     1081 gccggggcta tctattacaa catggacgag cgcgacgtcc gccgagtgtt gagttatccg
     1141 gtcagtatga ttggctctga cggcctgccc aatgatccca tgccgcatcc acgtttatgg
     1201 ggcgccttcc ctcgcgtgct gggccactat tgccgtgatg aaggcttatt cccactgacc
     1261 acggctatcc acaaaatgac cgggctttct gccagccgtt ttcgcctgcc ccagcgtgga
     1321 ctggtgaaag tcggttattt tgcggacctg gtgttgttcg atccacaaac cattcgtgat
     1381 gttgccagct tttctgatcc caaacgcccg gcagatggca ttgaggctgt gatggtgaac
     1441 ggcgttatga gctatggtcc cgataaacat attacaggcc gtgcgggacg cttcctgcgc
     1501 cggcagacct cacattaagg agtgaatatg agcattaaac gttacggtac ggaaggtgga
     1561 acaggcactg gcgggcagca tttgcctttt gccagggcgg ttgaggcggg cggctggctg
     1621 tacgtctccg ggcaaacacc catgaaaaac ggtgaagtgg tagaaggcgg aatcgttgaa
     1681 cagtcacgac tggcgatcca aaactgtgtc gacatcatga ctgaagccgg ttatagcctg
     1741 gcggatgtgg ttcacgtgaa agtgatcctg accgacgccc gctatttcca gtcatttaat
     1801 aaagtattca gagaattttt tggcgagaat ccgcctgcac gtatttgttg tgtggctgat
     1861 ctggttgtcg attgtaaagt cgaagtcgat gtgacctgtt ataacgcgac ccgctaataa
     1921 atttaagaat taaaaaaaca gaccgcaatt gagtgctgaa tacattcagc actccgggaa
     1981 ctcttacatt aatttctctc tactggcagg atgtgaatta tgaacagtca tatcttttta
     2041 gtcggcttta ttatttatgc catcgccatg atttggcttg gttggtttgt ttctcgtaat
     2101 cagaaaagcg gagaagattt tctgctgggc gggcgttcgc tgccgctgtt tctgacgctg
     2161 gggtcaaccg ttgccacgat ggttggcacg ggttccagta tgggggcagt gggctttggc
     2221 tacagcaatg gctgggccgg aatgctttat ggcgtcggtg gagccgtagg tatattgctg
     2281 gtggcgtggc tgtttgcacc ggtacgtaaa ttacgtttta tgaccatgag tgaagaactc
     2341 tcttattata ctggcggtag tcatt
//
LOCUS       DAZFLD010000387         2363 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.389, whole genome shotgun sequence.
ACCESSION   DAZFLD010000387 DAZFLD010000000
VERSION     DAZFLD010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2363
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.389"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            111..1631
                     /gene="aer"
                     /locus_tag="ACHKBL_004866"
     CDS             111..1631
                     /gene="aer"
                     /locus_tag="ACHKBL_004866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerotaxis sensor receptor Aer"
                     /protein_id="HFO5821600.1"
                     /translation="MSSHPYVTQQNTPLADDTTLMSTTDLQSYITHANDTFVQVSGYT
                     LQELQGQPHNMVRHPDMPKAAFADMWFTLKKGEPWSGIVKNRRKNGDHYWVRANAVPM
                     VREGKISGYMSIRTRATDEEIAAVEPLYKALNAGRTSKRIHKGLVVRKGWLGKLPSLP
                     LRWRARGVMTLMFILLTAMLWFVAAPVVTYILCALVVLLASACFEWQIVRPIENVARQ
                     ALKVATGERNSVEHLNRSDELGLTLRAVGQLGLMCRWLINDVSSQVSSVRNGSETLAK
                     GTDELNEHTQQTVDNVQQTVATMNQMAASVKQNSATASAADKLSITASNAAVQGGEAM
                     TTVIKTMDDIADSTQRIGTITSLINDIAFQTNILALNAAVEAARAGEQGKGFAVVAGE
                     VRHLASRSANAANDIRKLIDASADKVQSGSQQVHAAGRTMEDIVAQVKNVTQLIAQIS
                     HSTLEQADGLSSLTRAVDELNLITQKNAELVEESAQVSAMVKHRASRLEDAVTVLH"
     gene            complement(1735..2358)
                     /gene="nfeR"
                     /locus_tag="ACHKBL_004867"
                     /gene_synonym="yqjI"
     CDS             complement(1735..2358)
                     /gene="nfeR"
                     /locus_tag="ACHKBL_004867"
                     /gene_synonym="yqjI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NfeR"
                     /protein_id="HFO5821601.1"
                     /translation="MSHHHEGCCKHEGQPRHEGCCKGEKSEHEHCGHGHQHEHGQCCG
                     GRHGRGGGRRQRFFGHGELRLVILDILSRDDSHGYELIKAIENLTQGNYTPSPGVIYP
                     TLDFLQEQSLITIREEEGGKKQIALTEQGAQWLEENREQVEMIEERIKARCVGAALRQ
                     NPQMKRALDNFKAVLDLRVNQSDITDAQIKKIIAVIDRAAFDITQLD"
BASE COUNT          558 a          606 c          669 g          530 t
ORIGIN      
        1 atcgcaaatt gcgatctaaa tcaaattaat cagttaaaga taaccgcagc ggggccgaca
       61 taaactctga caagaagtta acaaccatat aacctgcaca ggacgcgaac atgtcttctc
      121 atccgtatgt cacccagcaa aataccccgc tggcggacga taccactctg atgtccacta
      181 ccgatctgca aagctatatc actcatgcta atgacacttt tgtgcaggtg agcggctata
      241 ccttgcaaga gttacaaggt caaccgcaca acatggtgcg tcacccggat atgccaaaag
      301 cggcgtttgc ggatatgtgg ttcaccctga aaaaagggga gccctggagc ggcatcgtga
      361 aaaatcgccg caaaaatggc gaccattatt gggtgcgggc caatgcggta ccgatggtgc
      421 gcgagggaaa aatcagtggc tatatgtcga ttcgtacccg ggcgacggat gaagagatcg
      481 cggcggtgga gccgctgtac aaagcgttga acgccggacg taccagtaag cgtattcata
      541 aaggcctggt ggtgcgtaaa ggatggctgg gtaaactgcc ttcattaccg cttcgctggc
      601 gggcgcgtgg cgtgatgacc ctgatgttta tcttgctgac ggccatgcta tggtttgttg
      661 ctgccccggt ggtgacgtat atcctctgtg cgttagtggt attattggca agcgcttgtt
      721 ttgaatggca gattgtccgc ccgatagaaa atgtcgcccg tcaggcactg aaggtggcga
      781 ccggagagcg taatagtgtt gagcatctga atcgcagcga tgagctgggg ctgacattac
      841 gcgcggtagg gcagcttggc ctgatgtgcc gttggctaat taacgatgtc tcaagccagg
      901 tgtccagtgt cagaaacggc agtgagacgc tggcgaaagg caccgatgaa ctgaacgaac
      961 atacccagca gacagttgat aacgttcagc aaacggtggc gaccatgaac caaatggcgg
     1021 cgtcggtgaa acagaactct gccacggcgt cggctgccga taaactttct atcactgcca
     1081 gtaatgcggc agtgcagggt ggggaggcga tgactacggt gatcaagaca atggacgata
     1141 ttgccgacag tacccagcgc attggcacca ttacttcgct gattaacgat attgcgtttc
     1201 agaccaatat tctggccctg aatgcggcgg tggaagcggc gcgtgccggc gaacagggca
     1261 aaggttttgc agtggtggcg ggggaagtgc gtcatttagc tagccgcagc gccaatgctg
     1321 ccaacgatat tcgcaagctg attgatgcca gtgctgataa ggtgcaatcc ggttcgcagc
     1381 aggtacacgc cgccggacgg acgatggaag atattgtggc acaggtaaaa aacgtcaccc
     1441 agttgatagc tcagattagc cattcaacgc tggaacaggc cgatgggctt tccagcctga
     1501 cccgtgcagt ggatgagctt aacctgatca cccagaaaaa cgccgagtta gtggaagaga
     1561 gtgcgcaggt gtcggcgatg gtgaaacacc gcgccagccg actggaagac gcggtgacgg
     1621 tgctgcatta atcgatatgc cgcatccggc ggttggcgca caatgcctga tgcgatgctg
     1681 gcgcatctta tcaggcctac gggggtaggg cacgccacat ccggcaatcg gtgattaatc
     1741 cagttgcgta atatcaaaag cggcgcggtc gatcaccgca atgatctttt ttatttgtgc
     1801 atcagtgata tcgctctggt tgacgcgtaa atccagtacc gctttaaaat tatccagcgc
     1861 ccgcttcatt tgcgggttct ggcgcagcgc agcgccaacg caacgcgctt tgatgcgttc
     1921 ttcaatcatc tctacctgtt cgcggttttc ttccagccac tgcgcgcctt gttcggtcag
     1981 cgcaatctgc ttcttaccac cttcctcttc gcgaatggta atcagcgact gctcctgcag
     2041 aaaatccagc gttgggtaga tgacgcccgg gcttggggtg taattcccct gggtcagatt
     2101 ttcaatcgct ttaatcaact cgtaaccgtg gctgtcatcg cgcgagagaa tatccagaat
     2161 caccagacgc aattcaccgt gaccaaagaa acgttgtcga cgaccgccgc cgcgaccgtg
     2221 gcgaccaccg cagcattgac cgtgttcatg ctggtgaccg tgtccgcagt gctcgtgttc
     2281 tgacttctca cctttgcagc agccctcatg gcgtggctgg ccttcatgtt tacagcaccc
     2341 ttcgtgatga tggctcatat cag
//
LOCUS       DAZFLD010000388         2358 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.390, whole genome shotgun sequence.
ACCESSION   DAZFLD010000388 DAZFLD010000000
VERSION     DAZFLD010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2358
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.390"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            595..864
                     /gene="yacA"
                     /locus_tag="ACHKBL_004868"
     CDS             595..864
                     /gene="yacA"
                     /locus_tag="ACHKBL_004868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015056448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin YacA"
                     /protein_id="HFO5821602.1"
                     /translation="MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQ
                     HNTWFRDQVAAGRQQLEHGDVLPHDMVESSAAAWRDKMSRKVAGK"
     gene            864..1142
                     /gene="yacB"
                     /locus_tag="ACHKBL_004869"
     CDS             864..1142
                     /gene="yacB"
                     /locus_tag="ACHKBL_004869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013009346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin YacB"
                     /protein_id="HFO5821603.1"
                     /translation="MEIFWTMLASQDRKRIREYIAEQNLMAAIELDERIGYSASSLAG
                     QPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKVYILRVLHTAQKWP"
     gene            1188..2036
                     /locus_tag="ACHKBL_004870"
     CDS             1188..2036
                     /locus_tag="ACHKBL_004870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001057990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HFO5821604.1"
                     /translation="MNNDELATRRAQAIAEDRCFSKGRLRDEFRMKPAPGAEPVKWYK
                     NTYGGRFAVYRIADCVPMREKRPLTSKQLLAGQRLSVLSRLNSTSGRMARQAYDWLSL
                     APLFLDTETTGLDNTAEALEIGLTDAAGQVVFETRLKPTVAIGAQAAAVHGISEQALC
                     GAPSWTDVARQLRHAIGGRPVIIFNARFDIRILKQTAAAHSDPAYWLEEMTVYCAMEL
                     AAGYYGATNRYGTISLASAASQAGLTWEGLAHSAIADARMTAGVVNAIAAYHLELLQE
                     QARLEI"
     gene            2153..>2358
                     /locus_tag="ACHKBL_004871"
     CDS             2153..>2358
                     /locus_tag="ACHKBL_004871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001402899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5821605.1"
                     /translation="MRDKTREAMRLFLGGRCYTAEKLEKDYLAEVANYSNDRWEAPQR
                     ASRLAASVKRYKTSEMLRFIFATIA"
BASE COUNT          602 a          589 c          655 g          512 t
ORIGIN      
        1 ttccatttaa aaagaaaacc tattcaatgc ctgtcctgtg gacaggcaaa tatgtacctc
       61 ccactctaac gcgcgggccc cgaacctcac cgttctgaaa ccacagcaaa aaaacatcag
      121 gaataaaaac actacacaaa cgcagcaccg tgcccacccc tcataactga aaagcgaggc
      181 cgcccccgcc caaagggcgg gaacaacatc gcttttaatt atgaatgttg taactaaaca
      241 acaccatcgc tgtcagtctt ctggctggac gtactgaata cacgctcgta agcggccctg
      301 acggcccgct aacgcggaga tacgccccga cttcgggtaa accctcgtcg ggaccactcc
      361 gaccgcgcac agaagctctc tcatggctga aagcggatat ggcttagcag ggtgggaatg
      421 ggataggcga aatctatcaa tcagtaccgg cttacgccgg gcttcggcgg ttttactccg
      481 gtatcatatg aaacaatgga gtgccgcctt ccatgccgct ggcgcggcat atcctggtga
      541 cgatatctga atcgttatat actgtgtata tacgtagtaa tgacgaggtg ataaatggca
      601 caggttaata tgagtttaag aatcgacgct gaactgaagg atgcttttat ggccgctgca
      661 aaaagcatgg accgtaatgg ctctcagtta atccgggatt ttatgcgcca gaccgttgaa
      721 cggcagcata atacctggtt ccgcgaccag gttgcggcag gacgtcagca actcgagcac
      781 ggcgatgtgc ttccccatga catggtcgaa tcctctgctg cagcatggcg ggataaaatg
      841 agcaggaagg tcgccggtaa atgatggaga tattctggac tatgctggcc agccaggaca
      901 ggaagcgcat tcgtgagtac attgcggagc agaacctgat ggccgccatt gaactggacg
      961 aacggattgg ttattcagcc agcagcctgg ccgggcagcc gtataaaggc cgcaacggtc
     1021 gtgtggaagg tacccgggaa ctggttattc acccgcattt tgttctggtt tatgaggtcg
     1081 acagccagtg gggaaaagtg tatatcctgc gtgtattgca tactgcacag aagtggccat
     1141 agagaggctg tccggatata aaaacgtctg acggagatgc aagaaccatg aataatgatg
     1201 agctggccac cagacgtgcg caggcgattg ctgaagacag atgtttctcg aaaggacgtc
     1261 tgcgcgatga attcagaatg aagcctgccc ccggtgctga gccggttaag tggtataaaa
     1321 acacttatgg tggcaggttc gctgtatacc gaattgcaga ttgtgttccg atgcgtgaga
     1381 aacgcccact gacgagtaaa caacttctgg ctggccagcg actttccgtt ttatccagac
     1441 ttaacagcac cagtggccgg atggcacggc aggcatatga ctggctgagt ctggcaccgc
     1501 tctttctgga tactgaaacg acaggactgg ataataccgc ggaagccctg gaaatcggcc
     1561 ttactgatgc ggccggtcag gtggtatttg aaaccagact gaagcccacg gtcgccatcg
     1621 gggcgcaggc cgccgctgtg catggtatca gtgagcaggc tctctgcggc gcaccatcat
     1681 ggacagatgt ggcgcggcaa ctgcgtcatg ctatcggggg ccgaccagta attatcttta
     1741 atgcccggtt cgacatccgc attctgaaac agactgctgc cgcacatagc gatccggctt
     1801 actggctgga agaaatgacg gtatattgtg cgatggagct ggcagcagga tattatgggg
     1861 ccaccaaccg ctatggcacg atttcactgg ccagtgctgc cagccaggcc ggactgacct
     1921 gggaagggct ggcacactca gcgatcgccg acgcacggat gacggcaggg gtggtaaacg
     1981 ctattgctgc atatcatctg gaactgctgc aggaacaggc acggctggaa atctgaccgc
     2041 ctggcctgta taccgcaatc atttacgtta tccggacgta aatctgccgc ttgttgctgt
     2101 cagcatgtta atgctaatgt atgcgtacat tagcattaat gagggatgat atgtgagaga
     2161 caaaaccagg gaggccatgc ggcttttttt aggcggcagg tgctatacgg ctgaaaaact
     2221 tgaaaaggat tatctggctg aagtggctaa ttacagtaac gaccgctggg aagcaccgca
     2281 acgtgcctca cgcctggctg ccagcgtaaa gcgttacaag acatcagaaa tgctgcgctt
     2341 tatttttgct accattgc
//
LOCUS       DAZFLD010000389         2355 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.391, whole genome shotgun sequence.
ACCESSION   DAZFLD010000389 DAZFLD010000000
VERSION     DAZFLD010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2355
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.391"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            76..909
                     /gene="xapA"
                     /locus_tag="ACHKBL_004872"
     CDS             76..909
                     /gene="xapA"
                     /locus_tag="ACHKBL_004872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0055086 - nucleobase-containing small
                     molecule metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthosine phosphorylase"
                     /protein_id="HFO5821606.1"
                     /translation="MSQAQFSHNPLYCVDIIKTYKPDFTPRVAFILGSGLGALADQIE
                     NAVAISYEKLPGFPVSTVHGHAGELVLGYLQGVPVACMKGRGHFYEGRGMTIMTDAIR
                     TFKLLGCELLFCTNAAGSLRPEVGAGSLVALKDHINTMPGTPMVGLNDERFGERFFSL
                     ANAYDAEYRALLQKVAKEEGFPLTEGVFVSYPGPNFETAAEIRMMQIIGGDVVGMSVV
                     PEVISARHCELKVVAVSAITNMAEGLSDVKLSHAQTLAAAELSKQNFINLICGFLRKI
                     A"
     gene            970..2226
                     /gene="xapB"
                     /locus_tag="ACHKBL_004873"
     CDS             970..2226
                     /gene="xapB"
                     /locus_tag="ACHKBL_004873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthosine/proton symporter XapB"
                     /protein_id="HFO5821607.1"
                     /translation="MSIALRLKVMSFLQYFIWGSWLVTLGSYMINTLHFTGANVGMVY
                     SSKGIAAIIMPGIMGIIADKWLRAERAYMLCHLVCAGVLFYAASVTDPDMMFWVMLVN
                     AMAFMPTIALSNSVSYSCLAQAGLDPVTAFPPIRVFGTVGFIVAMWAVSLLHLELSSL
                     QLYIASGASLLLSAYALTLPKIPVAEKKATTSLASKLGLDAFVLFKNPRMAIFFLFAM
                     MLGAVLQITNVFGNPFLHDFARNPEFADSFVVKYPSILLSVSQMAEVGFILTIPFFLK
                     RFGIKTVMLMSMVAWTLRFGFFAYGDPSPTGFILLLLSMIVYGCAFDFFNISGSVFVE
                     QEVDSSIRASAQGLFMTMVNGVGAWVGSILSGMAVDYFSVDGVKDWHTIWLMFAGYAL
                     FLAVIFFFGFKYNHDPEKITHRSVTH"
     gene            2279..>2355
                     /locus_tag="ACHKBL_004874"
     CDS             2279..>2355
                     /locus_tag="ACHKBL_004874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XapX domain-containing protein"
                     /protein_id="HFO5821608.1"
                     /translation="MKDILFSSGVGFGIGALFTIVRLPIP"
BASE COUNT          494 a          511 c          639 g          711 t
ORIGIN      
        1 aagtgtattg gtatgaggta gggcaatctc gttataaaaa ggcgttagat tccaccctac
       61 aaaaaaagga tatatatgtc tcaggctcag ttttctcata acccactgta ttgcgtagat
      121 attatcaaga cttataaacc tgatttcacg ccacgagtgg cctttatttt aggttccggg
      181 ctgggcgcgc tggccgatca gattgagaac gcggtcgcaa tttcctacga aaagctgcct
      241 gggttcccgg taagtaccgt acacggtcat gcgggtgagc tggtgctggg gtatctccag
      301 ggggtgccag tggcgtgcat gaaaggtcgc ggacatttct acgaaggtcg tgggatgacc
      361 atcatgacgg atgcaatccg tacctttaag ttgctgggct gcgagttgct gttctgcacc
      421 aatgcggctg gctcactgcg tcctgaagtg ggggccggca gtctggtcgc attgaaagat
      481 cacatcaaca ccatgccggg aacgccgatg gtgggtctta atgatgaacg ttttggtgag
      541 cgcttcttct cgctggcgaa tgcctacgat gcggaatacc gcgcactgtt acaaaaagtg
      601 gcgaaagaag aggggttccc tctgacggag ggcgtgttcg tctcgtatcc ggggccgaat
      661 ttcgagactg cggcggaaat tcgtatgatg caaattattg gtggggatgt tgttggtatg
      721 tctgtggtgc ctgaggttat ttcagctcgc cattgcgaac ttaaagtcgt tgcggtctct
      781 gcgattacca acatggcgga aggtctgagt gacgtgaagc tttctcatgc ccaaacgctg
      841 gcagcagcgg aactctcaaa gcaaaacttt attaatctta tttgcggctt tctgcgcaaa
      901 attgcctgag taaaacaaca ataaacggcc ttactgagtg aggccatatg atgcgacaag
      961 gaaacgatta tgagcatcgc tttgcgctta aaggtaatgt cctttttgca atattttatc
     1021 tgggggagct ggctggttac cctcggttct tacatgatta atacccttca tttcaccggc
     1081 gctaatgttg gcatggttta cagttccaaa gggatcgccg cgattattat gccgggtata
     1141 atggggatca tcgcagacaa atggctgcgt gcagaacgtg catacatgct gtgtcacctg
     1201 gtgtgtgcgg gcgtactttt ttatgcggca tccgtaactg atccggatat gatgttttgg
     1261 gtgatgttag tcaatgcgat ggcgtttatg ccgactattg cgttatcgaa cagcgtctct
     1321 tattcctgtc ttgcccaggc agggcttgac ccggtgaccg ctttcccgcc cattcgcgtt
     1381 tttggtacgg tggggttcat tgtcgccatg tgggcagtaa gcctgctgca tctggaattg
     1441 agtagtctgc agctgtatat cgcgtccggt gcgtcattgc tgctgtcggc ttatgcgctg
     1501 actttgccga agattccggt tgcggagaaa aaagcgacta catcgcttgc cagcaagctg
     1561 ggtctggatg ccttcgtgct gtttaaaaat ccacgcatgg ctatcttttt cctcttcgcc
     1621 atgatgctgg gcgcggtgct gcaaattacc aacgtttttg gcaacccgtt cctgcatgat
     1681 ttcgcccgta atcctgagtt tgctgatagc ttcgtggtga aatatccgtc cattttactg
     1741 tcagtttcac agatggctga agtcggtttc attctgacga taccgttctt tttgaagcgt
     1801 ttcggtatta aaaccgtcat gctgatgagt atggtggcgt ggacgctgcg ctttggcttc
     1861 tttgcctatg gcgacccatc accaaccggg tttattttgc tgctgctgtc gatgattgtt
     1921 tatggctgtg cattcgattt cttcaatatt tctggttcgg tatttgtcga acaggaagtt
     1981 gattccagca ttcgtgccag cgcccagggc ctttttatga ccatggtaaa tggtgtcggc
     2041 gcatgggttg gctcgattct gagcggtatg gcagtggatt acttctcggt ggatggcgta
     2101 aaagattggc ataccatttg gctgatgttt gcagggtatg ctcttttcct cgcagtgata
     2161 tttttctttg ggtttaaata taaccatgac cctgaaaaga taacgcatcg ttcagttaca
     2221 cattaaaagg attcgcagct ctgttccttg gggctgcttt tatgataaag gttaattagt
     2281 gaaagatatt ttattttctt ctggcgttgg ttttggcatt ggtgctttgt ttacaatcgt
     2341 gcgcttgcct attcc
//
LOCUS       DAZFLD010000390         2350 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.392, whole genome shotgun sequence.
ACCESSION   DAZFLD010000390 DAZFLD010000000
VERSION     DAZFLD010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2350
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.392"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>2350
                     /gene="ypjA"
                     /locus_tag="ACHKBL_004875"
     CDS             <1..>2350
                     /gene="ypjA"
                     /locus_tag="ACHKBL_004875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="adhesin-like autotransporter YpjA/EhaD"
                     /protein_id="HFO5821609.1"
                     /translation="NSGTQNIKSGGKADTTNISSGSRQVVEKDGTATGSNISAGGSLI
                     VYTGGIAHGVNQETGSALVANTGAGTDIEGYNKLSHFTITGGEANYVVLENTGELTVM
                     AKTSAKNTTIDAGGKLIVQKEAKTDSTRLNNGGVLEVQDGGEAKHVEQQSGGALIAST
                     TSGTLIEGTNSYGDAFYIRNSEAKNVVLENAGSLTVVTGSRAVDTIINANGKMDVYGK
                     DVGTVLNSAGTQTIYASATSDKANIKGGKQTVYGLATDANIESGEQIVDGGSTDKTHI
                     NGGTQTVQNYGKAINTDIVSGLQQIMANGTAEGSIINGGSQVVNEGGLAENSVLNDGG
                     TLDVREKGSATGIQQSSQGALVATTRATRVTGTRADGVAFSIEQGAANNILLTNGGVL
                     TVESDTTSAKTQVNAGGREIVKTKATATGTTLTGGEQIVEGVANETTINDGGIQTVSA
                     NGEAIKTTINEGGTLTVNDNGKATDIVQNSGAALQTSTANGIEISGTHQYGTFSIAGY
                     LATNVLLENGGNLLVLAGTEARDSTVGNGGAMQNLGQDFATKVNSGGQYTLGRSKDEF
                     QVLARAEDLQVAGGTAIVYAGTLADASVSGATGSLSLMTPRDNVTPVKLEGAVRITDS
                     ATLTLGNGVDTTLADLTAASRGSVWLNSNNSCAGTSNCEYRVNSLLLNDGDVYLSAQT
                     AAPATTNGIYNTLTTSELSGSGNFYLHTNVAGSRGDQLVVNNNATGNFKIFVQDTGVS
                     PQSDDAMTLVKTGGGDASFTLGNTGGFVDLGTYEYVLKSDGN"
BASE COUNT          662 a          531 c          664 g          493 t
ORIGIN      
        1 tcaacagcgg aacacaaaat attaagagcg gcgggaaagc tgacacgaca aatatatcct
       61 ctgggagccg gcaggttgtt gagaaagatg gtacggcaac tggcagcaat attagcgccg
      121 gaggctcgct gattgtctat accggcggta ttgcacatgg ggttaaccag gagacgggca
      181 gtgctttagt tgccaacacg ggcgcaggga ctgatatcga aggatacaat aagctctctc
      241 acttcactat taccggaggt gaggctaatt atgttgtgct ggaaaatacc ggcgaactga
      301 cggtaatggc taaaacctcg gcgaaaaata ctaccattga tgctggcggt aagctgattg
      361 tccagaagga ggctaaaaca gatagcacca gacttaataa tggtggcgtt ctggaggttc
      421 aggacggtgg tgaggctaag catgttgagc aacaatccgg cggcgcatta attgcttcca
      481 cgacttccgg aacccttatc gaaggaacca acagttatgg tgatgctttc tacatcagga
      541 attcagaagc taaaaatgta gtgctggaaa acgctggctc attaacagtc gtcactggtt
      601 cccgggcagt tgatacgatt attaatgcca acggcaaaat ggatgtttat ggaaaagatg
      661 ttggcactgt actcaatagt gcgggcaccc aaacaatata tgccagtgcc acttctgata
      721 aagcaaatat caaaggtggc aagcaaacgg tatatggttt agccactgat gcaaatattg
      781 aaagtggtga acaaattgtt gatggtgggt caacagacaa aacgcacatc aatggcggca
      841 cacaaaccgt tcagaattat ggtaaggcga tcaataccga tatcgtctct ggcttacaac
      901 aaattatggc aaacgggaca gcggaaggtt ccattattaa tggcggttca caggtagtta
      961 atgagggcgg tctggctgaa aactcggtgc ttaatgacgg cggcacactc gatgtgcggg
     1021 agaaaggcag cgcaacgggg atacagcaga gtagtcaggg cgcgttggtt gcaaccacca
     1081 gggcgacgcg ggtcacaggg acacgcgctg atggcgtcgc gttcagcatc gagcagggtg
     1141 cggcgaacaa tatcctgctg acaaatggcg gcgtgttaac cgtggagtca gacaccactt
     1201 ccgccaaaac acaggtcaat gcgggcggtc gggagatcgt caaaacaaaa gccactgcga
     1261 ctggcacgac gctcactggc ggtgaacaaa tcgtcgaggg tgtggcaaat gagacaacaa
     1321 ttaacgacgg cggaatacaa acagtttcag ctaacggaga ggcaataaaa acaacgatca
     1381 atgaaggcgg aacgctgaca gtcaacgata atggcaaagc gacagatatc gtccagaaca
     1441 gcggtgccgc tctccagacg agcacggcta acggtattga aatcagcggt acgcaccagt
     1501 acggcacctt ttccattgcc ggatatttag cgaccaatgt actgctggaa aatggcggta
     1561 atttattggt attagcaggt accgaagctc gcgattccac ggttggcaac ggtggggcaa
     1621 tgcaaaacct ggggcaggac ttcgccacaa aggttaattc tggtgggcaa tataccctcg
     1681 gtcggtcaaa agatgagttt caggttctgg cccgggcaga agatctacag gttgctggcg
     1741 ggacggcaat cgtctacgca ggtacgctgg cggatgcatc ggtcagtggc gcgacaggaa
     1801 gcctgtcgtt aatgacgcca cgggataatg ttacgccagt aaaacttgaa ggggcggtcc
     1861 ggattaccga tagcgcgaca ttaactctgg gaaatggtgt cgatacgacg cttgccgacc
     1921 tgacggctgc cagccggggc agtgtctggc ttaacagcaa taattcctgt gcaggcacca
     1981 gcaactgcga atatagagta aatagtttgc tcctcaacga cggtgatgtt tatttatcag
     2041 cacaaacagc agcgcctgcc acaactaatg gtatctacaa tacgctgacc accagtgaac
     2101 tttccggcag cggtaatttc tacctgcata ccaacgttgc aggctcccgg ggcgatcaac
     2161 tggtcgtcaa caacaacgcc actggcaatt ttaaaatctt tgttcaggat accggcgtca
     2221 gccctcagtc tgacgacgcg atgacgctgg tgaaaacagg gggaggggat gcttcgttta
     2281 cgctgggtaa taccggcggt ttcgttgatc ttgggaccta tgagtatgtc ctgaaaagcg
     2341 atggcaacag
//
LOCUS       DAZFLD010000391         2327 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.393, whole genome shotgun sequence.
ACCESSION   DAZFLD010000391 DAZFLD010000000
VERSION     DAZFLD010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2327
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.393"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..284
                     /locus_tag="ACHKBL_004876"
     CDS             <1..284
                     /locus_tag="ACHKBL_004876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO5821610.1"
                     /translation="SEPGTKWTANKVARYLYISVSTLHRRLASEGVSFQSILDDVRLN
                     NALSAIQTTVKPISEIARENGYKCPSRFTERFHNRFNITPREIRKASRE"
     gene            401..502
                     /locus_tag="ACHKBL_004877"
     CDS             401..502
                     /locus_tag="ACHKBL_004877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5821611.1"
                     /translation="MRTYNPNSLLPSQMQKCTCNSLHLAFDLCGGEA"
     gene            499..954
                     /gene="ybcN"
                     /locus_tag="ACHKBL_004878"
     CDS             499..954
                     /gene="ybcN"
                     /locus_tag="ACHKBL_004878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA base-flipping protein YbcN"
                     /protein_id="HFO5821612.1"
                     /translation="MNLSQDGIKLHRGNFTAIGRQIQPYLEEGKCFRMVLKPWREKRS
                     LSQNALSHMWYSEISEYLISRGKTFATPAWVKDALKHTYLGYETKDLVDVVTGDITTI
                     QSLRHTSDLDTGEMYVFLCKVEAWAVNIGCHLTIPQSCEFQLLRDKQEA"
     gene            954..1124
                     /locus_tag="ACHKBL_004879"
     CDS             954..1124
                     /locus_tag="ACHKBL_004879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HFO5821613.1"
                     /translation="MATPLIRVMNGHIYRVSNRRKRKPELKPSEIPTLLGYTASLVDK
                     KWLRLAARRNHG"
     gene            1117..1407
                     /locus_tag="ACHKBL_004880"
     CDS             1117..1407
                     /locus_tag="ACHKBL_004880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HFO5821614.1"
                     /translation="MADLRKAARGRECQVRIPGVCNGNSETSVLAHIRLAGLCGTGIK
                     PPDLIATIACSACHDEIDRRTHFVDAAYAKECALEGMARTQVIWLKEGVIKA"
     gene            1404..1766
                     /gene="rusA"
                     /locus_tag="ACHKBL_004881"
     CDS             1404..1766
                     /gene="rusA"
                     /locus_tag="ACHKBL_004881"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RusA"
                     /protein_id="HFO5821615.1"
                     /translation="MNTYSITLPWPPSNNRYYRHNRGRTHVSAEGQAYRDNVARIIKN
                     AMLDIGLAMPVKIRIECHMPDRRRRDLDNLQKAAFDALTKAGFWLDDAQVVDYRVVKM
                     PVTKGGRLELTITEMGNE"
     gene            1763..1903
                     /locus_tag="ACHKBL_004882"
     CDS             1763..1903
                     /locus_tag="ACHKBL_004882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcG family protein"
                     /protein_id="HFO5821616.1"
                     /translation="MMFEFYMAELLRHRWGHLRLYRFPGSVLTDYRILKNYAKTLTGA
                     GV"
     gene            1989..>2327
                     /gene="quuD"
                     /locus_tag="ACHKBL_004883"
     CDS             1989..>2327
                     /gene="quuD"
                     /locus_tag="ACHKBL_004883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein QuuD"
                     /protein_id="HFO5821617.1"
                     /translation="MRDIQMVLERWGAWAANNHEDVTWSSIAAGFKGLITSKVKSRPQ
                     CCDDDAMIICGCMARLKKNNSDLHDLLVDYYVVGMTFMSLAGKHCCSDGYIGKRLQKA
                     EGIIEGMLMAL"
BASE COUNT          632 a          519 c          586 g          590 t
ORIGIN      
        1 tatccgaacc cggcacaaaa tggacagcaa acaaggttgc tcgatatctc tacatttctg
       61 tttctacatt acatcgccgt ctagcaagcg agggggtaag tttccaaagt atactggacg
      121 atgtgaggtt aaataatgcg ttgtctgcta tacaaacgac ggtaaaacct ataagcgaga
      181 ttgccagaga aaatggttat aagtgtcctt ctcgttttac tgaaagattt cataatcgtt
      241 ttaatataac accaagagag ataagaaaag cttccagaga gtaaaagtgt tttaagaagg
      301 agcaattcta tcgattttga ttttgggaaa tcaacacggc ataattatgt caccggagcc
      361 tgaacaactc cggtgacttc tgcgctaaac ggggacgttt atgcgcacat acaatccaaa
      421 ctctcttctc ccttcacaga tgcagaaatg cacctgcaat tctttgcatc tagcgtttga
      481 cctctgcgga ggtgaagcgt gaacctctca caagacggca tcaaattaca tcgcggcaac
      541 ttcaccgcta tcggtcggca gatccagcct tatctggagg agggcaaatg ctttcgcatg
      601 gtgcttaaac cgtggcgtga gaaacgcagt ctttcccaga atgcactcag ccacatgtgg
      661 tacagcgaaa tcagtgaata cctcatcagc aggggtaaaa cgttcgccac tccagcttgg
      721 gtaaaagatg ctctcaaaca cacatatctc ggttatgaaa ccaaagacct ggttgatgtc
      781 gtaaccggtg atatcaccac tatccagtcg ttacgccata cctccgatct tgataccgga
      841 gagatgtatg tcttcctgtg taaggttgaa gcctgggcgg tgaatattgg ctgccacctg
      901 actattccgc agagctgcga gttccagctg ctccgcgaca agcaggaggc gtaatggcta
      961 caccgcttat tcgtgtcatg aacggacaca tctacagagt atcaaatcgt cgtaagcgta
     1021 agcctgagct gaagccatcc gaaataccaa cactgctcgg atataccgct agcctggttg
     1081 ataaaaaatg gttgcgactg gcagcaagga ggaatcatgg ctgatttgag aaaagcagcg
     1141 cgtggtcggg aatgccaggt aagaatccct ggcgtatgta atggcaattc tgaaacgtct
     1201 gtactggcac atatccggct ggctggattg tgcggtaccg gtatcaaacc gccagacctg
     1261 attgccacca ttgcatgttc tgcctgccac gacgaaatcg accgccgcac acattttgtc
     1321 gatgctgcat atgcaaaaga atgcgcgctg gaaggtatgg cgagaacaca ggttatctgg
     1381 ctgaaagagg gggttattaa ggcgtgaata cctacagtat cacattaccc tggcctccga
     1441 gcaataatcg ctattaccgc cataatcgcg ggcgcacgca cgtcagtgca gaggggcagg
     1501 cataccgcga taacgtcgcc cgaatcatta aaaacgcaat gctggatatc ggcctggcta
     1561 tgcctgtgaa aatccgcatt gagtgccaca tgccggatcg ccgtcgccgt gacctggata
     1621 atctgcaaaa agccgctttt gacgcactca ccaaagcagg tttctggctg gatgatgctc
     1681 aggtcgttga ttaccgcgtt gtgaagatgc ctgttaccaa aggtggcagg ctggaactga
     1741 ccatcaccga aatggggaat gaatgatgtt tgagttttat atggcagaac ttcttcgcca
     1801 ccgctggggg catctgcgct tatatcgttt ccccggttct gttttgaccg attaccgaat
     1861 actgaagaat tacgccaaaa ccctgacagg agcaggagta tgaagtcaga gataacaatc
     1921 aactaatact gttttgttga tttttgcttg taattggcgt tctggtctga tttttgtgga
     1981 gtaagttgat gcgtgatatt cagatggttc ttgagcgttg gggagcgtgg gcggctaata
     2041 atcatgaaga tgtgacctgg tcgtccattg ccgccggttt taagggatta attacttcaa
     2101 aagtaaaatc tcgcccgcaa tgttgtgacg atgacgcgat gattatttgc gggtgcatgg
     2161 cccgtctgaa aaagaacaac agcgatttgc acgatttatt agtagattat tatgtagtcg
     2221 gtatgacatt catgtcactg gcaggtaagc attgctgctc tgatggttat atcgggaaaa
     2281 ggttacagaa ggctgagggc ataattgaag ggatgttaat ggcatta
//
LOCUS       DAZFLD010000392         2321 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.394, whole genome shotgun sequence.
ACCESSION   DAZFLD010000392 DAZFLD010000000
VERSION     DAZFLD010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2321
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.394"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..2320
                     /locus_tag="ACHKBL_004884"
     rRNA            <1..2320
                     /locus_tag="ACHKBL_004884"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          598 a          506 c          735 g          482 t
ORIGIN      
        1 aatgggtcag cgacttatat tctgtagcaa ggttaaccga ataggggagc cgaagggaaa
       61 ccgagtctta actgggcgtt aagttgcagg gtatagaccc gaaacccggt gatctagcca
      121 tgggcaggtt gaaggttggg taacactaac tggaggaccg aaccgactaa tgttgaaaaa
      181 ttagcggatg acttgtggct gggggtgaaa ggccaatcaa accgggagat agctggttct
      241 ccccgaaagc tatttaggta gcgcctcgtg aattcatctc cgggggtaga gcactgtttc
      301 ggcaaggggg tcatcccgac ttaccaaccc gatgcaaact gcgaataccg gagaatgtta
      361 tcacgggaga cacacggcgg gtgctaacgt ccgtcgtgaa gagggaaaca acccagaccg
      421 ccagctaagg tcccaaagtc atggttaagt gggaaacgat gtgggaaggc ccagacagcc
      481 aggatgttgg cttagaagca gccatcattt aaagaaagcg taatagctca ctggtcgagt
      541 cggcctgcgc ggaagatgta acggggctaa accatgcacc gaagctgcgg cagcgacact
      601 aagtgttgtt gggtagggga gcgttctgta agcctgtgaa ggtgtactgt gaggtatgct
      661 ggaggtatca gaagtgcgaa tgctgacata agtaacgata aagcgggtga aaagcccgct
      721 cgccggaaga ccaagggttc ctgtccaacg ttaatcgggg cagggtgagt cgacccctaa
      781 ggcgaggccg aaaggcgtag tcgatgggaa acaggttaat attcctgtac ttggtgttac
      841 tgcgaagggg ggacggagaa ggctatgttg gccgggcgac ggttgtcccg gtttaagcgt
      901 gtaggctggt tttccaggca aatccggaaa atcaaggctg aggcgtgatg acgaggcact
      961 acggtgctga agcaacaaat gccctgcttc caggaaaagc ctctaagcat caggtaacat
     1021 caaatcgtac cccaaaccga cacaggtggt caggtagaga ataccaaggc gcttgagaga
     1081 actcgggtga aggaactagg caaaatggtg ccgtaacttc gggagaaggc acgctgatat
     1141 gtaggtgaag cgacttgctc gtggagctga aatcagtcga agataccagc tggctgcaac
     1201 tgtttattaa aaacacagca ctgtgcaaac acgaaagtgg acgtatacgg tgtgacgcct
     1261 gcccggtgcc ggaaggttaa ttgatggggt tagcgcaagc gaagctcttg atcgaagccc
     1321 cggtaaacgg cggccgtaac tataacggtc ctaaggtagc gaaattcctt gtcgggtaag
     1381 ttccgacctg cacgaatggc gtaatgatgg ccaggctgtc tccacccgag actcagtgaa
     1441 attgaactcg ctgtgaagat gcagtgtacc cgcggcaaga cggaaagacc ccgtgaacct
     1501 ttactatagc ttgacactga acattgagcc ttgatgtgta ggataggtgg gaggctttga
     1561 agtgtggacg ccagtctgca tggagccgac cttgaaatac caccctttaa tgtttgatgt
     1621 tctaacgttg gcccctgatc ggggttgcgg acagtgtctg gtgggtagtt tgactggggc
     1681 ggtctcctcc taaagagtaa cggaggagca cgaaggttgg ctaatcctgg tcggacatca
     1741 ggaggttagt gcaatggcat aagccagctt gactgcgagc gtgacggcgc gagcaggtgc
     1801 gaaagcaggt catagtgatc cggtggttct gaatggaagg gccatcgctc aacggataaa
     1861 aggtactccg gggataacag gctgataccg cccaagagtt catatcgacg gcggtgtttg
     1921 gcacctcgat gtcggctcat cacatcctgg ggctgaagta ggtcccaagg gtatggctgt
     1981 tcgccattta aagtggtacg cgagctgggt ttagaacgtc gtgagacagt tcggtcccta
     2041 tctgccgtgg gcgctggaga actgaggggg gctgctccta gtacgagagg accggagtgg
     2101 acgcatcact ggtgttcggg ttgtcatgcc aatggcactg cccggtagct aaatgcggaa
     2161 gagataagtg ctgaaagcat ctaagcacga aacttgcccc gagatgagtt ctccctgact
     2221 ccttgagagt cctgaaggaa cgttgaagac gacgacgttg ataggccggg tgtgtaagcg
     2281 cagcgatgcg ttgagctaac cggtactaat gaaccgtgag g
//
LOCUS       DAZFLD010000393         2315 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.395, whole genome shotgun sequence.
ACCESSION   DAZFLD010000393 DAZFLD010000000
VERSION     DAZFLD010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2315
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.395"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            131..634
                     /locus_tag="ACHKBL_004885"
     CDS             131..634
                     /locus_tag="ACHKBL_004885"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO5821618.1"
                     /translation="MNNIAPQSPVIRRLTLQDNPAIARVIRQVSAEYGLTADKGYTVA
                     DPNLDELYQVYSQPGHAYWVVEYEGEVVGGGGIAPLTGSEADICELQKMYFLPAIRGK
                     GLAKKLALMAMEQAREMGFKRCYLETTAFLKEAIGLYEHLGFQHIDYALGCTGHVDCE
                     VRMLREL"
     gene            complement(680..1096)
                     /gene="rraB"
                     /locus_tag="ACHKBL_004886"
     CDS             complement(680..1096)
                     /gene="rraB"
                     /locus_tag="ACHKBL_004886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HFO5821619.1"
                     /translation="MANPEQLEEQREETRLIIEELLEDGSDPDALYTIEHHLSADDLE
                     TLEKAAVEAFKLGYEVTDPEELEVEDGDIVICCDILSECALNADLIDAQVEQLMTLAE
                     KFDVEYDGWGTYFEDPNGEDGDDEDFVDEDDDGVRH"
     gene            1258..2271
                     /gene="argF"
                     /locus_tag="ACHKBL_004887"
     CDS             1258..2271
                     /gene="argF"
                     /locus_tag="ACHKBL_004887"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HFO5821620.1"
                     /translation="MSGFYHKHFLKLLDFTPAELNSLLQLAAKLKADKKSGKEEAKLT
                     GKNIALIFEKDSTRTRCSFEVAAYDQGARVTYLGPSGSQIGHKESIKDTARVLGRMYD
                     GIQYRGYGQEIVETLAEYAGVPVWNGLTNEFHPTQLLADLLTMQEHLPGKAFNEMTLV
                     YAGDARNNMGNSMLEAAALTGLDLRLVAPQACWPEAALVTECRALAQQNGGNITLTED
                     VAKGVEGADFIYTDVWVSMGEAKEKWAERIALLRDYQVNSKMMQLTGNPEVKFLHCLP
                     AFHDDQTTLGKKMAEEFGLHGGMEVTDEVFESAASIVFDQAENRMHTIKAVMVATLSK
                     LNN"
BASE COUNT          605 a          544 c          567 g          599 t
ORIGIN      
        1 tgccacgtta caatactctt atcagtaata ggctggcaaa aactttattg ctttataaga
       61 cgcttgcgca cgtaagaaga gagtggtatt aaacaaatgt ataattactt tatttttatt
      121 gggttaaact atgaataaca ttgcgccgca atcacctgta atacgtcgcc tgacgctgca
      181 ggataatcct gctatcgccc gcgtcattcg tcaggtatcc gccgaatacg gtcttaccgc
      241 tgataaaggc tacaccgtcg ccgatccgaa tcttgacgag ctgtatcaag tgtatagtca
      301 gcctggccat gcatattggg tggttgagta cgagggtgaa gtggtcggcg gcggtgggat
      361 agcgccatta acaggtagtg aagcggacat ttgcgaactg cagaaaatgt actttcttcc
      421 cgccattcgc ggtaaagggc tggcaaaaaa actggcctta atggcgatgg agcaggcgcg
      481 agaaatgggt ttcaaacgct gctatctgga aacgaccgct tttttgaaag aagcgattgg
      541 tttatatgaa catttaggtt ttcaacatat cgactatgca cttggctgca cgggccatgt
      601 tgattgtgaa gtgcggatgt tgcgcgaact ctgaaaaaat gcccgctagt atgtgctatc
      661 gggcatttta atcaattaat tagtggcgaa ccccgtcatc gtcttcatcg acaaaatctt
      721 catcgtcgcc atcttcgccg ttaggatctt caaagtaagt gccccaaccg tcgtactcaa
      781 cgtcaaattt ctccgccagc gtcatcagtt gttcaacctg ggcatcgatc agatcggcat
      841 tcaacgcgca ctcgctgagg atgtcgcagc aaatcacgat atcaccatct tcaacttcca
      901 gctcttctgg atcggtcact tcgtaaccga gtttaaacgc ttcaactgcc gctttttcca
      961 gagtttccag atcgtctgcg gaaagatggt gttcgatggt gtacagcgcg tccggatcgc
     1021 tgccatcttc cagtaattct tcaataatca aacgtgtttc ttcacgctgt tcttccagtt
     1081 gttccgggtt tgccatggct cattcctctt aatgtcctgc cgatactctt attgtcacac
     1141 accactgaca ttgcctccac ctttgtgata aagatttatg ctttagactt gcaaatgaat
     1201 aatcatccat ataaattgaa ttttaattca ttgaggcgtt agccacagga gggatctatg
     1261 tccgggtttt atcataagca tttcctgaaa ttactcgatt tcacgccagc tgaacttaac
     1321 agcctgctgc agttagccgc gaagctgaaa gctgataaga aaagcggtaa agaagaagcc
     1381 aaactcactg gtaaaaacat cgcgctcatc ttcgaaaaag actcgacccg tacccgatgc
     1441 tctttcgaag ttgccgcata tgaccagggt gctcgcgtta cttatctcgg cccaagcggt
     1501 agtcagattg gtcataaaga gtcgattaaa gacactgccc gcgtgcttgg tcgcatgtat
     1561 gacggtattc agtatcgcgg ctatggtcag gagattgtcg aaacactggc ggaatacgct
     1621 ggcgtgccgg tatggaacgg cctgaccaat gagtttcacc ccacgcagtt gctggcggat
     1681 ctcctcacca tgcaggagca tttacccggt aaagcattca acgaaatgac gctggtctat
     1741 gcaggtgacg cacgtaacaa catgggcaat tcgatgctcg aagctgcggc gcttaccggt
     1801 ctggatttgc gtctggtcgc gccacaggca tgctggccag aagctgcgct ggttacggaa
     1861 tgccgcgccc tggcacaaca aaatggcggg aatattacgc tgacggaaga tgtagctaaa
     1921 ggagttgaag gtgctgactt tatctatacc gatgtatggg tgtcgatggg tgaagcaaaa
     1981 gaaaaatggg ctgaacgcat tgcattactg cgtgattatc aggtgaacag caagatgatg
     2041 cagttgaccg gtaacccgga ggtcaaattc ctccactgcc tgcccgcgtt tcatgacgac
     2101 cagacgacgc ttggtaagaa aatggcggaa gagtttggcc tacatggcgg aatggaagtg
     2161 actgatgagg tcttcgaatc tgccgccagc attgtttttg atcaggcgga aaaccgcatg
     2221 cataccatca aagcggtgat ggtcgcgacg ctcagtaaat taaataatta atatctctat
     2281 aaagatgccg ggaaatacaa aaacatcccg gcatc
//
LOCUS       DAZFLD010000394         2294 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.396, whole genome shotgun sequence.
ACCESSION   DAZFLD010000394 DAZFLD010000000
VERSION     DAZFLD010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2294
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.396"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(457..750)
                     /locus_tag="ACHKBL_004888"
     CDS             complement(457..750)
                     /locus_tag="ACHKBL_004888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000559728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HFO5821621.1"
                     /translation="MITDSVAQKLEEHGLWRRAATRWSEVLLHAETDREREEAARRRA
                     ICIIKSRRMPEQFVTFGDVKKAADRTLKEMGINPQDEWRNYSFSDAGDDLALS"
     gene            complement(770..1186)
                     /locus_tag="ACHKBL_004889"
     CDS             complement(770..1186)
                     /locus_tag="ACHKBL_004889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001076063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ProQ/FINO family protein"
                     /protein_id="HFO5821622.1"
                     /translation="MNQQTTQQDQTTRRPTERPSWRHMTKRQRKNRRRINRLAEYWPE
                     LFGAETIKPLKTGIIRDVLEDAKSRELPIGHGVLRGALTSYIHTTRYLKAIVAGGPRY
                     GLNGQPCGEVTSDEKAVARGILKQRLEQMKQKNIQQ"
     gene            complement(1203..1652)
                     /locus_tag="ACHKBL_004890"
     CDS             complement(1203..1652)
                     /locus_tag="ACHKBL_004890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFO5821623.1"
                     /translation="MTAQIAAYGRLVDDPQVKQTSKGTAMTLARMAVSLPCSQAQDGQ
                     ATLWLSVLAFGKQADALARHEKGGLISVAGNMQISQWTGQNGETRQGWQVIADSVISA
                     RSARPGGKRGQQGKATEALERAKRQTGQQGNQPPAGGDDPFSDDIPF"
     gene            complement(1649..2098)
                     /locus_tag="ACHKBL_004891"
     CDS             complement(1649..2098)
                     /locus_tag="ACHKBL_004891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5821624.1"
                     /translation="MTEKHAKKSHSHAIDLTEPESVVRFVSVRGRATLYIPDEHLHHC
                     DKHKLPILIVWKRTVYADVTWLNDSLMLIHRDLFEREEFRRDIEERAEKIYEKYSANS
                     KRSARAIAHHFMTLYDLKAEDAEKAACDLFDMTMGIIQEYRNKERRP"
BASE COUNT          465 a          633 c          545 g          651 t
ORIGIN      
        1 gggtaataaa aaaccctgaa agatttctcc gggtgctcca gttgcacgtg gcagaaatca
       61 ttcagggtca gccgtaacgg gtctctgtac agagggcttc agttgcccgg agatgagcgc
      121 ccgttaccgc cagctgtgat aagccgcttt acagtgggct ccagttgccc aaagaggcgc
      181 gtttttatca caactttgcc gcgataaccc gctgtacaga gggcttcaat tgcccaaaga
      241 ggcgcattct tatcgcggca tgacgaaatc accgtgatgt tttcgtcgtt cactcatccc
      301 gatgtatggg agatacaccg gaatgagtgc cgaaaagccg ctggctggct tcattcagtc
      361 gggctccagt tgcccgagga gaacactatc aacggccttt cggtggtgat tatagcgaat
      421 cgcgataatt gagcaaatac ggcgatgaga tgcgtatcac gatagcgcta aatcgtcgcc
      481 tgcatcactg aagctgtaat ttcgccattc atcctgtgga ttaatcccca tctctttcag
      541 cgttctgtct gcggcctttt tcacatcgcc aaaggtgaca aactgctcag gcatacgacg
      601 ggatttaatg atacatatcg cccgacgtct ggcagcttcc tcgcgttccc tgtctgtttc
      661 tgcgtgaaga agaacctcag accatcgggt cgccgcacgt cgccataaac cgtgctcctc
      721 aagtttctgt gccacgctgt ccgttatcac tggctttacc tccggctgat tactgctgga
      781 tgtttttctg cttcatctgc tccagtcgct gtttcagtat cccacgggct acggcttttt
      841 catccgacgt cacttccccg caaggctgcc cgttcagtcc ataccgaggg ccaccagcga
      901 caatggcttt caggtatcgc gtcgtgtgga tgtatgaggt taatgcgcca cgtaatacgc
      961 catgaccaat cggtaattcc cgtgatttag cgtcctcaag cacatcacgt attatgcccg
     1021 ttttcagggg ctttatcgtc tctgccccaa acagttcagg ccagtactca gccagtcggt
     1081 ttatcctcct gcggtttttt cgctgccgct tcgtcatgtg tcgccatgat gggcgctccg
     1141 tgggtctgcg tgtggtctgg tcctgttgtg tggtctgctg gttcatgttg tgctcctgtc
     1201 attcagaaag gaatatcgtc agagaacggg tcatcacctc ccgctggtgg ctgatttccc
     1261 tgctgtccgg tctgtcgttt tgctctttcc agtgcttcgg tcgccttccc ctgttgccca
     1321 cgcttcccgc caggacgcgc cgaacgggca ctgattacgc tgtcagcgat aacctgccag
     1381 ccctgccgtg tttctccgtt ctgtccggtc cactgactga tctgcatgtt acccgccacg
     1441 ctgataaggc cgcctttttc atgtctcgcc agcgcgtcgg cctgcttacc aaatgccagg
     1501 actgatagcc ataacgtcgc ctgtccgtcc tgtgcctgac tgcatggcaa agataccgcc
     1561 atacgcgcca gcgtcatcgc tgttcccttg ctggtctgtt ttacctgcgg gtcgtccacc
     1621 agccgcccgt aagctgcaat ctgtgctgtc atggtctgcg ctccttgttc cggtactcct
     1681 ggataatccc cattgtcata tcgaataaat cacatgccgc tttttctgca tcttccgctt
     1741 tcaggtcata cagcgtcatg aaatggtgag cgattgcccg tgcggatctt ttgctgtttg
     1801 ctgaatattt ctcatagatt ttttctgccc gctcttcaat atcccgccgg aactcttcac
     1861 gttcaaacag gtcacggtgt atcaacatca gcgaatcatt cagccatgtc acatcggcgt
     1921 ataccgttcg cttccagaca atcaggatcg gaagtttatg tttgtcacag tgatgcagat
     1981 gttcgtcagg tatataaagc gtggctctgc ctcgtaccga tacgaacctc acaacagatt
     2041 ccggttcggt taaatcgata gcgtgtgaat gtgatttttt ggcgtgtttc tctgtcataa
     2101 aatttttctc aaaggaataa ataaaaattt atgcccaaaa ggtgttcaac tgttcacctt
     2161 ttgctatttc gcctttagat tcatgaagtt aaggggtgaa cactttttgt aaaggtgttc
     2221 actaactgtt caccctgttc acccgtgact gttcaaaaat catacaaaaa ggtgaacagg
     2281 tgaacacttt ggtg
//
LOCUS       DAZFLD010000395         2288 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.397, whole genome shotgun sequence.
ACCESSION   DAZFLD010000395 DAZFLD010000000
VERSION     DAZFLD010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2288
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.397"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            408..1046
                     /locus_tag="ACHKBL_004892"
     CDS             408..1046
                     /locus_tag="ACHKBL_004892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000846269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5821625.1"
                     /translation="MRQGNPVNFTEYASQWTEAQLERDDGNHSTPEMAQQWPFSGKRC
                     IRKGYSDYYPFGVDGSRQDDETEIKHAVTVILASWPLFNRDGLDLYNHNKAWEHPLDY
                     VCFMEEAKSCLRWIRENSLTNDKIASFPEKNPTSYGLKHCVERANKRLTLENGMPTEP
                     TYISNGAFIAAMVASGYKIKPAGRMNCRFNISHKQLKRVLSGELYKAGEWTI"
     gene            1043..1492
                     /locus_tag="ACHKBL_004893"
     CDS             1043..1492
                     /locus_tag="ACHKBL_004893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFO5821626.1"
                     /translation="MTAQIAAYGRLVDDPQEKQTSKGTPMTLARMAVSVPCHNADDGQ
                     ATLWLSVLAFGKQADQLARHEKGDLISVAGNMQISQWTGQNGEIRQGWQVIADSVISA
                     RSARPGGKRGQQGKATEALERAKRQTGQQGNQPPAGGDDPFSDDIPF"
     gene            1509..1976
                     /locus_tag="ACHKBL_004894"
     CDS             1509..1976
                     /locus_tag="ACHKBL_004894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001076062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ProQ/FINO family protein"
                     /protein_id="HFO5821627.1"
                     /translation="MNQQTTQQDQTTRKPSRRIPWQHMTKEQRKNHRRVNRLCEYWPE
                     LFNLSEVRPLKNGVISDMLQEVRTRNIPVGKGALRGAVITYTHMTRYLKALIAGGPRY
                     DLNGKQCGSVTDAEKAAAEEVLAKRLAEAKQRSNQRQPCGGDDSDNRQRGTET"
     gene            1945..2238
                     /locus_tag="ACHKBL_004895"
     CDS             1945..2238
                     /locus_tag="ACHKBL_004895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001303092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HFO5821628.1"
                     /translation="MITDSVAQKLEERGLWRRAATRWAEVLLHAGTDREREEAARRRA
                     ICISKTRRMPEQLVTFGDVKKAADRTLKEMGINPQDEWRNYSFSDAGDDLALS"
BASE COUNT          689 a          525 c          641 g          433 t
ORIGIN      
        1 aggaaattga taaatatcaa tattgcacaa aaagcacaca accccgtatc cgcctttatg
       61 tttttgattc taaagaaaaa aaacatgcag ggcggatacg ggcggatacg aaaaatcacg
      121 taacaccatg aatacaaaca atatttttct tcatatccgc ttatccgcca aaaacgcgtc
      181 tttataagtg aatatgtgaa gttaaaaaac agagagcaga gcgggagatt aatgccattt
      241 ttcccgcagt aaccacagag aggaatcgag taatgtgcaa agaaaaaaac ttttaccagt
      301 gcatgagcag cagagagcgc gaagacctga aaagttttgt gacacggtgc gagattcgcg
      361 gagatattca gagcctgagc aggacactga tcatgattgc gcactggatg cgccagggca
      421 accccgtgaa cttcacagaa tatgcgagtc agtggacaga ggcacaactg gaacgggacg
      481 acggtaatca ctcaacgccg gaaatggcgc agcaatggcc tttcagtggc aagcgatgta
      541 ttcgcaaggg gtactctgat tattatccgt tcggcgttga tggttcacgt caggatgacg
      601 aaacggaaat taaacacgct gtaacggtaa ttctggcctc atggcctctg tttaaccgtg
      661 acgggcttga tctgtacaac cataacaaag cctgggagca cccgcttgat tatgtgtgtt
      721 tcatggaaga ggcgaaaagc tgtttacgct ggataaggga gaacagcctc acaaatgaca
      781 agatagcgtc attccctgag aaaaacccga cttcatacgg gcttaagcat tgcgttgaac
      841 gggcaaataa gagactcacc ctggaaaacg gaatgccgac ggaaccaaca tacatttcaa
      901 atggtgcatt catcgcggca atggtggcat ccggttataa gataaaacca gcaggccgga
      961 tgaattgccg gttcaatatt tcgcataagc aacttaagcg ggttctgagt ggtgaattat
     1021 ataaagccgg agagtggaca atatgaccgc acagattgca gcttacgggc ggctggtgga
     1081 cgacccgcag gaaaaacaga ccagcaaggg cacaccgatg acgctggcgc gtatggcggt
     1141 atcagtaccg tgccataacg cagatgacgg acaggcgacg ctatggttat ccgtcctggc
     1201 atttggcaaa caggctgacc aactggcgag acatgaaaaa ggcgacctta tcagcgtggc
     1261 gggtaacatg cagatcagcc agtggaccgg acagaacgga gaaatacggc agggctggca
     1321 ggttatcgct gacagcgtaa tcagtgcccg ttcggcgcgt cctggcggga agcgggggca
     1381 acaggggaag gcgaccgaag cactggaaag agcaaaacga cagaccggac agcagggaaa
     1441 tcagccacca gcgggaggtg atgacccgtt ctctgacgat attcctttct gaatgacagg
     1501 agcacaacat gaaccagcag accacacaac aggaccagac cacacgtaaa ccatcacggc
     1561 gcataccgtg gcagcacatg acaaaagagc aacgaaaaaa ccacagacgc gtaaaccgtc
     1621 tgtgcgagta ctggcctgaa ctgtttaacc tctcggaagt caggcccctc aagaacggcg
     1681 taatcagcga tatgttgcag gaggtcagga caagaaacat acctgtaggc aaaggtgcat
     1741 tacgtggggc ggtaatcact tacacccaca tgacgcgata ccttaaggcg ctgattgctg
     1801 gtggaccacg ttacgacctg aacgggaagc agtgcggaag cgtgacggat gcagagaaag
     1861 cggcagcgga ggaagtgctg gctaagcgtc tggcagaggc aaagcagcgg agtaaccagc
     1921 ggcaaccatg cggaggtgat gacagtgata acagacagcg tggcacagaa acttgaggag
     1981 cgcggtttat ggcgacgtgc ggcgacccga tgggctgagg ttcttcttca tgcaggaacg
     2041 gacagggaac gcgaggaagc tgccagacgt cgggcgatat gcatcagtaa aacccgtcgt
     2101 atgcctgaac aactggtcac ctttggcgat gtgaaaaagg ctgctgaccg gacgctgaaa
     2161 gagatgggga ttaatccaca ggatgaatgg cgaaattaca gcttcagtga tgcaggcgac
     2221 gatttagcgc tatcgtgata cgcatctcat cgccgtattt gctcaattat cgcgattcgc
     2281 tataatca
//
LOCUS       DAZFLD010000396         2270 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.398, whole genome shotgun sequence.
ACCESSION   DAZFLD010000396 DAZFLD010000000
VERSION     DAZFLD010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2270
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.398"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>2270
                     /locus_tag="ACHKBL_004896"
     CDS             <1..>2270
                     /locus_tag="ACHKBL_004896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011310120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemagglutinin"
                     /protein_id="HFO5821629.1"
                     /translation="KITVSNGDDDKLVTAKNIADAINQSGWKIKAGSENGGKVEGDNK
                     EELINPGDTVTLKAGKNLSVKQSNGDFTFSVVDTPSFSSVQLNGKGNNGNVTLSSEGN
                     KLTLSGGNGAGQNGVVISGVASGLEGKELSTLTNTDKEMKNAASVADLKTAIDGVSTS
                     MQGAGFALKDSDDKEVKQSLGQAIKVSGDSNITTTAITNGDKGLKISLNNDLSVGSNK
                     APGSIKVNGTDGKEAVSLNGQDGTIKLTSAPAANGKSASATIAVNAGKGGLTDAVGTT
                     KTRLTYTPDGKSPAEELATMNDGLSFEGDDGKIIHKKLNEKLTIKGGAGDNEDVTDSN
                     IRVDANEKGDGLLVRMTRNLRDLASAVFGTENGPTTRIDNAGITITTPAAGGGQGNTV
                     SLTGSGLNNGGNQITSVASGLNGTALADAGGDVLNNAANIGDLKNALRDSSAALVNTG
                     FGLQADDGSKVNTSLGKHITVGGDGQNISTTVQNGSLTVRLADNIRLADDGSVSVGGT
                     RMDKNGLTIKDGPSVTVNGIDAAGRVISGVKDGVNDQDAVNVSQLNREISQATAATTW
                     ALKTESGDEAVAVSSQTVEVRHGQNTRVSAISKDDKGNYSYEIDVTGIPVEYTDSQGN
                     PLVNIGGRFYTQTEDAATGKLTMTPAESARVRISSEQPLVLTNVAAGNVSPVSTDAVN
                     GSQLASAARIAGGDNVVWKDGKAALAPDTFSELTTAGGGKTTVNGGVHKTPDNVADAV
                     NMLNREGTKYFKAASSG"
BASE COUNT          645 a          472 c          665 g          488 t
ORIGIN      
        1 aagattacag taagcaatgg tgatgacgat aagcttgtta ctgcgaaaaa tattgctgat
       61 gccataaacc agtctggctg gaaaattaaa gctggcagtg aaaatggtgg gaaagttgag
      121 ggggataata aagaggagct aattaatccc ggtgatacag ttacactgaa agccggtaaa
      181 aatctcagcg tgaaacaaag taacggtgat tttacattct ctgttgttga tactccgtca
      241 ttcagcagtg ttcagctgaa tggaaaagga aataacggaa atgtaacgct ctcatccgaa
      301 ggtaataagc tgacattgtc aggcggtaat ggtgccggac aaaatggtgt tgttatcagt
      361 ggtgtggcaa gtggactgga aggtaaggaa ctttcgacgt taacgaatac tgataaagag
      421 atgaaaaatg ccgccagtgt tgcagacctg aaaacggcaa ttgatggtgt gtccaccagt
      481 atgcagggtg caggtttcgc actgaaagac tctgatgata aggaagtgaa acagtctctt
      541 gggcaggcga ttaaagtatc cggggacagc aatatcacca ccacagcaat caccaatggg
      601 gataaggggt taaaaatatc cctgaataac gacctgagcg tggggagcaa taaggctccc
      661 ggcagcatca aagttaacgg gactgatggt aaggaggctg tgagtctgaa tggtcaggat
      721 ggcacgatta aactgacatc cgcgcctgct gcgaacggaa agtctgcgtc agcaaccatt
      781 gctgttaatg caggcaaagg tggattaaca gatgctgtcg gtaccacgaa gacacgtctg
      841 acttataccc cggatggaaa atcaccggca gaagagctgg cgaccatgaa tgacggtctt
      901 tcttttgagg gtgatgacgg aaagattatt cataaaaaac tgaatgaaaa actgaccatc
      961 aaaggtggtg ctggtgataa tgaggatgta acagacagta acattcgtgt tgatgcaaat
     1021 gaaaagggag atggtcttct cgtccggatg accagaaacc tccgtgattt ggccagcgcg
     1081 gtttttggta cagaaaacgg cccgacaacc cggattgaca atgcaggcat cacaatcaca
     1141 acacctgctg cgggcggtgg tcaggggaat acggtttccc ttaccggctc cggcctgaat
     1201 aacggtggaa atcagataac cagtgtggcc agtggtctga acggcactgc gcttgctgac
     1261 gccggtggag atgttctgaa taatgcggcc aatatcggtg acctgaaaaa tgctctccgt
     1321 gacagcagtg cggcactggt gaataccggc tttggtctgc aggcggatga cggaagcaag
     1381 gtgaacacgt ctctcgggaa gcatatcacg gttggtggtg acgggcagaa tatcagcacg
     1441 actgttcaga acgggagcct gacggtcaga ctcgctgata acattcgcct ggcagatgac
     1501 ggcagtgttt ctgtcggcgg aacccggatg gataaaaacg gactgaccat caaggatggc
     1561 ccgtcagtga ctgtgaacgg tattgatgcg gctggccggg ttatttccgg cgtgaaagac
     1621 ggggtgaatg accaggacgc cgttaacgtt tcacaactga accgtgaaat cagtcaggca
     1681 acggcagcaa cgacctgggc actgaagact gaatccggtg atgaggctgt tgctgtttct
     1741 tcccagacag ttgaagtccg ccacggacag aatacccggg tttcagccat cagtaaggat
     1801 gacaagggaa attacagcta tgagattgat gtgacgggga ttccggtgga gtacaccgac
     1861 agccagggga acccgcttgt caatattggt ggtcgttttt acacacagac cgaagacgct
     1921 gcgaccggga aactgacaat gacgccggct gaatcggccc gtgtcagaat aagcagtgag
     1981 cagcctcttg ttctgacaaa tgtggcggcc ggaaatgtgt caccggtatc cacggatgcg
     2041 gttaacggga gccagctggc atctgcagcc agaattgctg gcggtgataa tgttgtctgg
     2101 aaagacggaa aagcagcgct ggctcctgac accttcagtg agctgacaac tgcaggtggc
     2161 ggtaagacaa cggtgaatgg tggtgtacat aaaacaccgg ataatgtggc tgatgcagtg
     2221 aatatgctga accgtgaagg cacgaagtat tttaaagctg cctcttccgg
//
LOCUS       DAZFLD010000397         2255 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.399, whole genome shotgun sequence.
ACCESSION   DAZFLD010000397 DAZFLD010000000
VERSION     DAZFLD010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2255
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.399"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..354
                     /gene="radC"
                     /locus_tag="ACHKBL_004897"
     CDS             <1..354
                     /gene="radC"
                     /locus_tag="ACHKBL_004897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFO5821630.1"
                     /translation="AAREWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTE
                     VHPREVIKRALYHNAAAVVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLI
                     VGGSQVFSFAEHGLL"
     gene            423..644
                     /locus_tag="ACHKBL_004898"
     CDS             423..644
                     /locus_tag="ACHKBL_004898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HFO5821631.1"
                     /translation="MKIITRGEAMRIHQQHPASRLFPFCTGKYRWHGSAGAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            720..1073
                     /locus_tag="ACHKBL_004899"
     CDS             720..1073
                     /locus_tag="ACHKBL_004899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HFO5821632.1"
                     /translation="MSDTLPDGNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAG
                     IRGLFSDADAYHLDQAFPLLMKQLELMLTSGELNPRHQHTVTLYARGLTCEADTLGSC
                     GYVYMAVYPTPETKK"
     gene            1163..1537
                     /locus_tag="ACHKBL_004900"
     CDS             1163..1537
                     /locus_tag="ACHKBL_004900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TA system toxin CbtA family protein"
                     /protein_id="HFO5821633.1"
                     /translation="MKTLPVLPGQSASSRPSPVEIWQTLLTRLLDQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSAGTSSQLINSIDILRARRATG
                     LMTRDNYRTVNNITLGKHPEKR"
     gene            1534..2022
                     /locus_tag="ACHKBL_004901"
     CDS             1534..2022
                     /locus_tag="ACHKBL_004901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001499210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
                     /protein_id="HFO5821634.1"
                     /translation="MKLSLMLEADRINVQALNMGQIVVDVDGVNLTELINKVAENGYS
                     LRVVEESDQQSTCTLPPFATLAGIRCSTAHITEKDNAWLYSLSHQTSDVGESEWIHFT
                     GSGYLLRTDAWSYPVLRLKRLGLSKTFRRLVVTLIRRYGVSLIHLDASAGCLPGFPTF
                     DW"
     gene            2034..2231
                     /locus_tag="ACHKBL_004902"
     CDS             2034..2231
                     /locus_tag="ACHKBL_004902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HFO5821635.1"
                     /translation="MKSLTTETALDILIAWLQDNIDCESGIIFDNDEDKTDSAALLPC
                     IEQAREDIRTLRQLQFLQQNR"
BASE COUNT          530 a          630 c          568 g          527 t
ORIGIN      
        1 gcggcacggg aatggctgat tctgaacatg gcgggactgg agcgtgaaga attccgggtg
       61 ctgtatctga acaaccagaa tcagttgatt gccggtgaaa ccctcttcac cggcaccatc
      121 aaccgcacgg aagtccatcc ccgggaagtg attaaacgcg ccctgtacca caatgccgct
      181 gccgtggtac tggcacacaa tcacccgtcc ggtgaagtca cacccagtaa ggcagaccgg
      241 cttattacgg aacgtctggt acaggcactg ggcctggtgg atatccgggt gccggaccat
      301 ctgatagtcg gtggcagcca ggttttctcc tttgccgaac acggtctgct ttaacccgtc
      361 accgtcacaa ccacatcata cctgttttca cttttatctt ctgtcttcag aggtatcaca
      421 tcatgaaaat catcacccgt ggtgaagcca tgcgtattca ccaacagcat ccggcctccc
      481 gtctttttcc gttctgcacc ggtaagtacc gctggcacgg cagtgccggg gcgtataccg
      541 gtcgtgaagt acaggatatt cccggtgtgc tggccgtgtt tgctgaacgc cgtaaggaca
      601 gttttggccc gtatgtccgg ctgatgagcg tcaccctgaa ctgagtcagg gcattctgat
      661 gagcagaatt atcgccacca ccggaccatt cttaaccgat tttctgtgag gattttatcg
      721 tgtcagacac acttcccgac ggcaatcacg accgcccctg gtgggggctg ccctgcaccg
      781 tgacgccctg tttcggcgcc cgtctggtgc aggagggtaa ccggttgcat taccttgccg
      841 accgcgctgg tatcagaggc ctgttcagcg atgcagatgc gtaccacctg gaccaggcct
      901 ttccgctgct gatgaaacaa ctggaactca tgctcaccag cggtgaactg aatccccgcc
      961 atcagcatac cgtcacgctg tatgccagag ggctgacctg cgaagccgac accctcggca
     1021 gttgtggcta cgtttatatg gctgtttatc cgacgcccga aacgaaaaag taactctcca
     1081 gaataaccct ctgctccggc ctggtgtttt caccacacca ctttcccatt tttcatcttt
     1141 gcatatcagg aaaatcttca gtatgaaaac attacctgta ttacccgggc agtcggccag
     1201 ttcacgccca tctcctgttg aaatctggca gacactgctc acccgactgc tggaccagca
     1261 ctatggcctt acgctgaatg acacaccgtt cgctgatgaa cgtgtgattg agcagcatat
     1321 tgaggcaggc atttcactgt gtgatgcggt gaattttctc gtggaaaaat acgcgctggt
     1381 gcgtaccgac cagccgggat tcagcgcagg aacttcgtcg cagttaatca acagcattga
     1441 tattctccgg gcacgccggg caacaggtct gatgacccgc gacaattaca gaacggtaaa
     1501 taacattacc ctgggtaagc atccggagaa acgatgaaac tttccctgat gctggaagcc
     1561 gacagaatta atgtgcaggc actgaacatg gggcaaattg tcgttgacgt cgatggtgtt
     1621 aatctcactg aactgattaa caaggtcgct gaaaacggtt attcactccg cgtggtggag
     1681 gaatccgacc aacagtcaac ctgcacacta ccaccgtttg caacccttgc cggcatacgc
     1741 tgcagtaccg cacatatcac ggaaaaggat aacgcctggc tgtactcgct gtcgcatcag
     1801 accagtgacg ttggtgaatc agaatggatt catttcacag gtagcggata tctgttacgt
     1861 accgatgcgt ggtcataccc ggttctgcgg cttaaacgcc tggggctgtc aaaaacgttc
     1921 cgccgtctgg ttgtcacact catccgacgt tatggcgtca gtctcattca tctggatgcc
     1981 agtgccggat gtctgccggg tttccccact ttcgactggt aaccagaaac aacatgaaat
     2041 cattaaccac ggaaacagcg ctggatattc tgattgcatg gctgcaggac aatatcgact
     2101 gcgaatcggg aattatcttt gacaacgatg aggataaaac ggattcggca gcactgctgc
     2161 cctgcattga acaggccagg gaagatatcc gtaccctgcg ccaactgcag tttctgcaac
     2221 agaaccggtg agtcttactc accagacttc attac
//
LOCUS       DAZFLD010000398         2242 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.400, whole genome shotgun sequence.
ACCESSION   DAZFLD010000398 DAZFLD010000000
VERSION     DAZFLD010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2242
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.400"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            112..393
                     /locus_tag="ACHKBL_004903"
     CDS             112..393
                     /locus_tag="ACHKBL_004903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012540180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5821636.1"
                     /translation="MNTTCARLPSADTLAEQAAKTASGWMSDAVIAIDELFGKGYAKQ
                     HPELVAAFMQTAGLDEAAMHLRGLVLANQRIDDTLEVLSEHIMLLNEPQ"
     gene            462..884
                     /locus_tag="ACHKBL_004904"
     CDS             462..884
                     /locus_tag="ACHKBL_004904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013813178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HFO5821637.1"
                     /translation="MRRDMQLVLERWGRWAASEEYCSMVDWPSMSVTSRDVTDSGKPG
                     CSDEDGAVIDTCIAHMSMVCPYDDLLILGLRFIGGLSTRAIAEAIDRSHLSVRTSLKA
                     SEAFLEGALTLQGVRLDMDPEVVLPERVVCAQKNMLWL"
     gene            970..1065
                     /locus_tag="ACHKBL_004905"
                     /pseudo
     CDS             970..1065
                     /locus_tag="ACHKBL_004905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000024321.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
     gene            1054..1130
                     /locus_tag="ACHKBL_004906"
     tRNA            1054..1130
                     /locus_tag="ACHKBL_004906"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1088..1090,aa:Arg,seq:tct)
     gene            1691..>2242
                     /locus_tag="ACHKBL_004907"
     CDS             1691..>2242
                     /locus_tag="ACHKBL_004907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HFO5821638.1"
                     /translation="MTFKHYDVVRAASPSDLADALAQKIREGWQPYGGPFSSYTDDGA
                     ALIQAIVAEGDVSTPVVVKPTGGEGAVISATSDPEYYFVVVLAGQSNSMAYGEGLPLP
                     ETYDRPDPRIKQLARRSTVTPGGAACKYNDIIPADHCLHDVQDMSRLNHPKADLSKGQ
                     YGTVGQGLHIAKKLLPFIPANAGI"
BASE COUNT          587 a          497 c          607 g          551 t
ORIGIN      
        1 agtaagagta tgatttttat gttttttagg ttactcaacc ttttgcccgt aaaaaaaggt
       61 tgagtaaaga aaacttgcag aaaatcacaa ataaccaaac ggagtaaaaa catgaataca
      121 acctgtgcaa gattaccaag tgcggatacg ttagcggaac aggcagcaaa aacggcaagt
      181 gggtggatgt cagacgccgt aatagcgata gatgagcttt ttggaaaggg ctatgcgaag
      241 caacaccctg aattagttgc tgcctttatg caaacagcgg gcttagatga agctgcaatg
      301 cacttacggg gacttgtgtt agctaaccaa cggattgatg acacactgga agtgctttcg
      361 gagcatatta tgctgcttaa cgagccccaa taaacccgat gcggataaac agacgtagtt
      421 ctcagaaaat tttttcgtag caaaacgtag aggccagcgt tatgcgtaga gatatgcaac
      481 ttgttctgga gcggtggggg cgatgggcgg cgagcgagga atactgttca atggttgact
      541 ggccctcaat gtcggttact tcccgggacg taaccgatag tggcaaaccc ggttgctctg
      601 acgaggacgg agcggtgatt gatacctgca tcgcgcatat gagtatggtt tgcccgtacg
      661 atgatttgct gatcctgggg ttacgtttta taggcggcct ctcaacgcgt gcaattgctg
      721 aggcgataga ccgtagtcat cttagcgtac ggacatccct gaaggcgtct gaagcatttc
      781 tggaaggagc tctgactctg cagggagtaa ggttggatat ggatcctgaa gttgtactac
      841 ctgaaagggt tgtgtgcgca caaaaaaata tgttatggtt atagcgtcta taattgtatg
      901 cttactgctg aaaggttccg ataacaccgg aacctttttt cttgtcttct gctgggaaaa
      961 attaataaaa caggaaagaa aaacatgaca tcgcactggt tcagtgtcag ccagtggcag
     1021 ttaccaaacg aggatgatta cagaaaactt catgcgttgt tagctcagcc ggacagagca
     1081 attgccttct aagcaatcgg tcactggttc gaatccagta caacgcgcca cattgatttt
     1141 tctgtctcgc ttcggtgggc ctttttgtcg ctgaaaaaga aaacaacggg tggttagcag
     1201 agtatcagtt aatcattgaa atttttaaat gcctcacaat ttcgtaagtt gatgattgtc
     1261 tggctgatgg cgaatttgtt aaaaatcgca tcgcatggcg aatccccctg tgcggagggg
     1321 cgactggtga aaaatcctta cttgtgattc attatcgacg cgggttcggt ggcaccgggc
     1381 cgaactcacc gggaggcacc cggcaccatg tacatcatga tacagatacg cagcgtcagc
     1441 ccctctccgg aggggctttt ttatggataa aaaaagcccg cgccaggaga cgcgggcggc
     1501 aaggaataaa caacaaaacg tgaagtaata tttcagctgg cgaataatac ccgacagtaa
     1561 tcactctgcg caactgcgcg gcctttttcg tattgcgggc tgtagtcttc cttctgtcat
     1621 tgtcctttaa cttccggacc tcagcccgct ctttatctga ttcagtacac tatcccggcc
     1681 gggaggattc atgacattta aacactacga tgtggtcagg gcggcgtcgc cgtcagacct
     1741 tgctgatgca cttgctcaaa aaattcgtga aggatggcaa ccatacggcg ggccgttttc
     1801 ttcgtatacg gatgatggcg cagcacttat tcaggcgatt gtcgcagaag gtgatgtgag
     1861 cacacctgtt gtggtgaagc cgacaggtgg agaaggtgca gtaatcagcg ccaccagcga
     1921 ccccgagtat tactttgttg tggttctggc ggggcagtca aacagcatgg catatggtga
     1981 aggccttccg ctgccggaga catatgaccg tccggacccg cgtattaagc agctggcgcg
     2041 ccgcagtacg gtgacaccgg gtggtgccgc ctgtaagtat aacgacatta ttccggcgga
     2101 ccattgtctg catgatgtgc aggacatgag ccgtcttaac catccgaaag cggacctgtc
     2161 aaaggggcag tacggaaccg tggggcaggg gctgcatatc gccaaaaaac tgctgccgtt
     2221 tataccggcg aatgcgggca tt
//
LOCUS       DAZFLD010000399         2232 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.401, whole genome shotgun sequence.
ACCESSION   DAZFLD010000399 DAZFLD010000000
VERSION     DAZFLD010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2232
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.401"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..603
                     /locus_tag="ACHKBL_004908"
     CDS             <1..603
                     /locus_tag="ACHKBL_004908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFO5821639.1"
                     /translation="TRLIYPAESKQITLSVQNSGDNTSYLIQSWVDDSKTEKKSQDFV
                     ITPPLFMLPTRKEASLRIMFLGKTNLPTDRETLYWMNVKAIPPTDEKNTQKNTLQLAL
                     QNKIKLFYRPENLPVQPGKARNMLRFKYEGKQLKVINPSPYYLTLTGIKVQGSKLPNT
                     FVPPKSDITVSSAVTLAGEITYQTINDFGATTERQKGIMQ"
     gene            640..>2232
                     /locus_tag="ACHKBL_004909"
     CDS             640..>2232
                     /locus_tag="ACHKBL_004909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001622451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFO5821640.1"
                     /translation="MNLKLKRCEYWMAAQKQMKRVVPLLLVIMPACSIAGMRFNPAFL
                     SGDTEAVADLSRFEKGMTYLPGSYEVEVWVNDSPLLSRTVTFKADDENQLIPCLSLAD
                     LLSLGINKNALPEQALALSENSCLDLRIWFPDVHYMPELDAQRLKLTFPQAIIKRDAR
                     GYIPPEQWDNGITAFLLNYDFSGNNDRGDYSSNNYYLNLRAGINIGAWRFRDYSTWSR
                     GSNSAGKLEHISSTLQRVIIPFRSELTLGDTWSSSDVFDSVSIRGIKLESDENMLPDS
                     QSGFAPTVRGIAKSRAQVTIKQNGYVIYQTYMPPGPFEISDLNPTSSAGDLEVTIKES
                     DNSETVYTVPYAAVPILQREGHSKYSTTVGQYRSNSYNQKSPYVFQGELIWGLPWDIT
                     AYGGAQFSEDYRALALGLGLNLGVFGATSFDVTQANSSLVDGSKHQGQSYRFLYSKSL
                     VQTGTAFHIIGYRYSTQGFYTLSDTTYQQMSGTVVDPKTLDDKDYVYNWNDFYNLRYS
                     KRGKFQASVSQPFGNYGSMYLSA"
BASE COUNT          649 a          443 c          499 g          641 t
ORIGIN      
        1 acgcgattga tttatccggc agaaagtaag caaataacac tcagtgtgca aaatagcggt
       61 gacaacacca gttatttaat tcaatcatgg gttgatgaca gtaaaacgga aaaaaagagc
      121 caggattttg ttatcactcc tcccctcttt atgttgccaa cgcgtaaaga agcttcatta
      181 cgtatcatgt ttcttggtaa aactaatttg ccgaccgatc gtgaaacttt gtattggatg
      241 aatgttaaag caattccgcc aacagatgag aaaaatacac agaagaatac cttacagttg
      301 gcattacaaa acaaaatcaa gctattttat cgcccggaaa atctacctgt gcaaccagga
      361 aaagcacgaa atatgttgcg gtttaagtat gagggcaaac aactaaaagt tatcaatcct
      421 tcgccttatt acctgacact gacaggaatt aaagtacaag ggagtaaatt gcccaatacg
      481 tttgtaccac cgaaaagcga tattacggtc tcgtcggcag tgacgttggc tggtgaaatt
      541 acttatcaaa caattaatga ctttggcgct acgacggagc ggcagaaagg aataatgcag
      601 taacaacggg atagttcaga tgcaacacag gtcagggata tgaacctaaa gctcaaaaga
      661 tgcgaatatt ggatggcggc acaaaagcag atgaaacggg ttgtaccgct tcttctggtt
      721 attatgcctg catgttcaat cgcgggaatg cgctttaacc ctgcttttct gtcgggtgat
      781 actgaagctg ttgctgactt atcccgtttc gagaaaggga tgacttatct tcctggtagc
      841 tatgaagtcg aagtttgggt caatgattcc cctttactct ctcgtactgt aacttttaaa
      901 gcagacgatg agaatcaact gattccctgc ctttcacttg ctgacttatt aagccttgga
      961 attaacaaga atgcgctgcc agagcaggct ttggctttat ctgaaaatag ttgccttgat
     1021 ttgcgtatct ggtttcctga tgtgcattac atgccggagc tggatgcaca gagacttaaa
     1081 ctgacctttc cgcaggcgat aataaaacgt gacgcccgcg gatatattcc accagaacag
     1141 tgggataacg gtattacagc ttttttgctg aattatgact tttctggtaa taacgatcgt
     1201 ggtgattact cttcaaataa ctattattta aatcttcgcg ctgggatcaa tattggtgca
     1261 tggcgttttc gcgattattc aacctggagt cgtgggagta attcagcagg taaactggag
     1321 catatcagta gtacgttgca gcgcgttatt attcctttca gaagtgaatt aacgctagga
     1381 gatacatggt catcatcaga tgttttcgac agtgttagta ttcgtggcat aaaactggaa
     1441 tctgacgaaa atatgttgcc cgatagtcaa agtggtttcg ctcccacggt gcgcggaatt
     1501 gcgaaaagtc gcgctcaggt aacaatcaaa cagaatggtt atgtcattta tcaaacctat
     1561 atgccgccgg gaccgtttga gattagcgat cttaacccga catcatctgc gggagatctg
     1621 gaagttacca tcaaagagtc tgataattca gaaactgtct ataccgtacc ttatgccgct
     1681 gtccccatcc tgcaacgaga aggtcattca aaatattcta ctacggttgg ccaatatcga
     1741 agcaatagct ataaccagaa aagtccttat gtatttcagg gggaattaat ttggggttta
     1801 ccctgggata ttacggctta tggtggggca caattctctg aggattaccg ggcgttggcg
     1861 ctcggccttg gcctgaatct gggtgtattt ggtgcaacat cgtttgatgt tactcaggct
     1921 aacagttcgc ttgtggatgg gagcaaacat caagggcaat cttatcgttt tctttattcc
     1981 aaatcgttag ttcagacagg aacagcattc catattattg gctatcgtta ttcaacccag
     2041 ggcttttaca ctttaagtga tacgacatac caacaaatgt cagggactgt tgttgatcca
     2101 aaaacgttag atgataaaga ttacgtttat aactggaatg atttttataa cttgcgttat
     2161 agcaaacgtg gaaaatttca ggctagtgta tcgcaacctt tcggtaacta cgggtctatg
     2221 tatttatcgg ct
//
LOCUS       DAZFLD010000400         2225 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.402, whole genome shotgun sequence.
ACCESSION   DAZFLD010000400 DAZFLD010000000
VERSION     DAZFLD010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2225
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.402"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..128
                     /locus_tag="ACHKBL_004910"
     CDS             <1..128
                     /locus_tag="ACHKBL_004910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HFO5821641.1"
                     /translation="FYVRSVKAAMALGRRATGVELETERFEQTVREVQDLASQNG"
     gene            169..244
                     /locus_tag="ACHKBL_004911"
     tRNA            169..244
                     /locus_tag="ACHKBL_004911"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:202..204,aa:Ile,seq:cat)
     gene            252..328
                     /locus_tag="ACHKBL_004912"
     tRNA            252..328
                     /locus_tag="ACHKBL_004912"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:286..288,aa:Arg,seq:tcg)
     gene            342..418
                     /locus_tag="ACHKBL_004913"
     tRNA            342..418
                     /locus_tag="ACHKBL_004913"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:376..378,aa:Arg,seq:tct)
     gene            510..1469
                     /gene="stxA2b"
                     /locus_tag="ACHKBL_004914"
     CDS             510..1469
                     /gene="stxA2b"
                     /locus_tag="ACHKBL_004914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2b subunit A"
                     /protein_id="HFO5821642.1"
                     /translation="MKCILLKWVLCLLLGFSSVSYSREFTIDFSTQQSYVSSLNSIRT
                     EISTPLEHISQGTTSVSVINHTPPGSYFAVDIRGLDVYQARFDHLRLIIEQNNLYVAG
                     FVNTATNTFYRFSDFAHISVPGVTTVSMTTDSSYTTLQRVAALERSGMQISRHSLVSS
                     YLALMEFSGNAMTRDASRAVLRFVTVTAEALRFRQIQREFRLALSETAPVYTMTPEEV
                     DLTLNWGRISNVLPEFRGEGGVRVGRISFNNISAILGTVAVILNCHHQGARSVRAVNE
                     EIQPECQITGDRPVIRINNTLWESNTAAAFLNRRAHSLNTSGE"
     gene            1482..1745
                     /gene="stxB2b"
                     /locus_tag="ACHKBL_004915"
     CDS             1482..1745
                     /gene="stxB2b"
                     /locus_tag="ACHKBL_004915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000719322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2b subunit B"
                     /protein_id="HFO5821643.1"
                     /translation="MKKIFVAALFAFVSVNAMAADCAKGKIEFSKYNENDTFTVKVAG
                     KEYWTNRWNLQPLLQSAQLTGMTVTIKSNTCASGSGFAEVQFN"
BASE COUNT          566 a          491 c          555 g          613 t
ORIGIN      
        1 ttttttatgt ccgtagcgtc aaagcagcaa tggcgctggg gcgtcgtgca actggcgttg
       61 agctggagac tgaacgtttt gagcagacgg tcagggaagt tcaggattta gccagtcaga
      121 acggatgata ttgcaggatt agttacgtac cgttattatc ctgcgcccgg ccctttagct
      181 cagtggtgag agcgagcgac tcataatcgc caggtcgctg gttcaaatcc agcaagggcc
      241 accatcacat accgccatta gctcatcggg acagagcgcc agccttcgaa gctggttgcg
      301 cggggttcga gtcctcgatg gcggtccatt atctgtacac tgcgttgtta gctcagccgg
      361 acagagcaat tgccttctaa gcaatcggtc actggttcga atccagtaca acgcgccata
      421 cttatttttt ctggctcgct tttgcgggcc ttttttatat ctgcgccggg tctggtgctg
      481 attacttcag ccaaaaggaa tacctgtata tgaagtgtat attgttaaaa tgggtactgt
      541 gcctgttact gggcttttct tcggtatcct attcccggga atttacgata gacttttcga
      601 cccaacaaag ttatgtctct tcgttaaata gtatacggac agaaatatcg accccacttg
      661 aacatatatc tcaggggacc acatcggtgt ctgttattaa ccacacccca ccgggcagtt
      721 attttgctgt ggatatacga gggcttgatg tctatcaggc gcgttttgac catcttcgtc
      781 tgattattga gcaaaataat ttatatgtgg ccggattcgt taatacggca acaaatactt
      841 tctacaggtt ttcagatttt gcacatatat cagtgcccgg tgtgacaact gtttccatga
      901 caacggacag cagttatacc actctgcagc gtgtcgcagc gctggaacgt tccggaatgc
      961 aaatcagtcg tcactcactg gtttcatcat atctggcgtt aatggagttt agtggaaatg
     1021 ccatgaccag agatgcatcc agagcagttc tgcgttttgt cactgtcaca gcagaagcct
     1081 tacggttcag gcaaatacag agagaatttc gtctggcact gtctgaaact gctcctgttt
     1141 atacgatgac accggaagaa gtggacctca cactgaactg ggggagaatc agcaatgtgc
     1201 ttccggagtt tcggggagag ggtggtgtca gagtggggcg aatatccttt aataatatat
     1261 cagcgatact gggcacagtg gcggttatac tgaattgcca tcatcagggg gcgcgttccg
     1321 ttcgcgccgt gaatgaagag atacaaccag aatgtcagat aactggcgac aggccagtta
     1381 taaggataaa caatacgtta tgggaaagta ataccgcagc tgcttttctg aatcgcaggg
     1441 ctcactcttt aaatacatcc ggagaataac aggagttaaa tatgaagaag atatttgtag
     1501 cggctttatt tgcttttgtt tctgttaatg caatggcagc tgattgtgca aaaggtaaaa
     1561 ttgagttctc taagtataat gagaatgata cattcacagt aaaagtggcc gggaaagagt
     1621 actggactaa ccgctggaat ctgcaaccgc tactgcaaag cgcacagtta acaggaatga
     1681 cggtaacaat caaatcaaat acctgtgcgt caggttcagg atttgctgaa gtgcagttta
     1741 attaatatca gaagcattgc tggtttcgtg gtgtgcagca atgtagttac agtgtaatca
     1801 atgtcacaat tcagtcagtt gaaggttgtc tgcccgactg agaatttgtt aaaaaaatcc
     1861 tgcatggtga atccccctga gcggaggggc atatcagcgt cacaggtgtt tctgttttac
     1921 ctctatcctt tctgtgcggg ttcaggtgct gatactgaac tcaccgggag gcacccggca
     1981 ccatgcatga acggtacata gcgcatacac cagcccctct ccggaggggc tttcttgtgg
     2041 gcaaaaaaaa gcccgagtgg gttcgggcaa tagcatgaga tatacatttt tataatcgaa
     2101 tggattttaa ccaggattca taaggctgcg caactgcgcg gcctttttcg tatttcgggc
     2161 tgtagtcttc cttctgccat tgtcctgtaa cttccggact tcagcccgcc ccttatctga
     2221 ctcac
//
LOCUS       DAZFLD010000401         2220 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.403, whole genome shotgun sequence.
ACCESSION   DAZFLD010000401 DAZFLD010000000
VERSION     DAZFLD010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2220
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.403"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..793
                     /locus_tag="ACHKBL_004916"
     CDS             <1..793
                     /locus_tag="ACHKBL_004916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001165562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DotH/IcmK family type IV secretion protein"
                     /protein_id="HFO5821644.1"
                     /translation="PASPLVQEAASLDSPLNAGEIRELRGRMADNERAISAPVTSVVP
                     RISSLTVNLSPGASLPLVRTALHNVSVVTFTDINGSPWPQSDPPYNAAPGHFDVQFNG
                     NMVSVTPLRAWAAGNVTVYLKGLSVPVILNITSGETDTNTLSQEIDSRLDLRIPRQGP
                     ASPVVAVPSEKIALHDATLQAFLDGVPPDDPTVRRLKFSGNVPGTTIWQHGDDLLVRS
                     RAMLRDEFEQTLASADGTRLWKLPVTPLLTFSVNGQSIHVTPELE"
     gene            797..2128
                     /gene="traO"
                     /locus_tag="ACHKBL_004917"
     CDS             797..2128
                     /gene="traO"
                     /locus_tag="ACHKBL_004917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001272008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraO"
                     /protein_id="HFO5821645.1"
                     /translation="MSAEQDTAAKSGKKLSALLVLGGVIIIGGGYLLLTWLSPENSAS
                     LSSVNINSTTTGSGRTVVESQHYRDLLRADNERGAAEALRNNQTFIASLPRGLDTDHG
                     NNQRRDTQPADQAEGSGQPSTPPQADQTAREKQREALQKLLARINTQHPDARPPVVAA
                     AMWGTTGAPGISSHASPPGTGTSPQPRLQEASLATPAARTGIQLIPALTRVPAFIDTE
                     VDSDNAVSRVVASVPAGPWAGAVLYSQDARLAGKGMDIHFDRMMWRGMFLKVNAYALN
                     EHTGMSSVASDVNHRWFKHIVLPSVLGGVGSVGSLYKDANTQVIQGNYGSITGRVGMP
                     DGEALAGVIAGGMAERGSQLLTRQAESEPYRQVTVSRGEVVSILFVEPVMSNDISPPS
                     SGTAPSVPPPSGRPLTQEEAERQASDRIQAAVMRKQNEMRQRYGIQQESHP"
BASE COUNT          502 a          679 c          616 g          423 t
ORIGIN      
        1 acctgcctct ccccttgttc aggaagccgc atcactggat tcccccctga atgcaggtga
       61 aattcgggaa ctgcggggac gaatggccga taacgaacgg gccatcagcg cacctgtcac
      121 cagtgtggtg ccccgtatca gctccctcac ggtaaatctg tcacccgggg ccagtctgcc
      181 gcttgtccga accgcactgc ataacgtcag cgtggtaacg ttcacggaca tcaacggctc
      241 cccgtggcct cagtcggatc cgccttataa cgcggcaccc ggtcactttg atgtgcagtt
      301 caacggcaat atggtctccg tcactcccct gcgcgcctgg gctgcaggga atgtaacggt
      361 ctacctcaaa ggactgtctg taccggtcat tctgaatatc accagtggtg aaaccgacac
      421 caataccctg agtcaggaga tcgacagccg tctggatctg cgcattcccc gtcagggacc
      481 tgccagtcct gtggtagccg taccatcaga gaagattgcc ctgcatgatg ccaccctgca
      541 ggcctttctg gacggtgttc ctcccgatga tcccaccgtg cgtcggctga aattctccgg
      601 aaatgttccg ggcaccacca tctggcagca cggcgatgat ctgctcgtgc gatcgcgggc
      661 catgctgcgg gatgaatttg aacagacgct ggcatctgct gacggtacgc gtctgtggaa
      721 actgccggtg actcccttac tcaccttctc cgtgaatggt cagtccattc acgtcacccc
      781 tgaactggag taaatcatga gcgcagaaca ggacacagcc gcaaagtccg gcaaaaaact
      841 gtcagccctg ctggttctcg ggggcgtgat tatcatcggg ggcggatacc tgctgctcac
      901 ctggctttcc cccgaaaaca gcgcatcgct ctcgtcagtc aatatcaaca gcacgacaac
      961 aggcagcggg cggaccgtgg tggagtcaca gcactaccgg gatttactgc gtgctgacaa
     1021 tgagcgcggg gccgcagaag cgttacgtaa taaccagacg ttcatcgcca gtctgcccag
     1081 gggactggat actgatcacg gaaataatca aagaagggat actcagcctg ctgaccaggc
     1141 cgagggttct ggtcaaccat caacgccccc tcaggcagat caaacagccc gggaaaaaca
     1201 gcgggaagcg ttacaaaaac tgcttgcccg catcaacaca cagcatcctg acgcccgccc
     1261 cccggtagtg gcggctgcaa tgtggggtac cactggtgct cccgggataa gctcacacgc
     1321 ctcaccaccc ggtaccggca catcaccaca accacgtctg caggaggcca gtctggcaac
     1381 cccggcagct cgtacaggga tacagctgat accggcactg acccgcgtac ctgccttcat
     1441 tgatacggaa gtggactctg acaatgctgt ctcccgcgtg gtggcctccg tacctgccgg
     1501 cccatgggcc ggggccgtcc tctacagcca ggacgccaga ctcgccggca agggaatgga
     1561 tatccacttt gaccggatga tgtggcgggg catgtttctg aaagtcaatg catatgccct
     1621 gaatgaacat accggcatgt catccgtggc cagcgacgtg aaccaccgct ggtttaagca
     1681 tatcgttctg ccgtctgtgc tcggtggggt gggcagtgtc ggaagtcttt acaaagacgc
     1741 caacacacag gtcattcagg gaaactacgg cagcattacc ggcagggtgg gaatgcctga
     1801 tggcgaggcg ctggctggcg tcattgcagg cggcatggcc gaacggggca gtcagttgct
     1861 gacccgccag gcggagagcg aaccttaccg gcaggtgaca gtgtcacgcg gagaggttgt
     1921 ctccattctg tttgttgaac cggtcatgag caacgatatc agtcccccct cctccggcac
     1981 tgccccttct gtgccaccgc cctccggacg cccgctcacc caggaagaag cagagcgtca
     2041 ggcgtctgac aggatccagg cagctgtcat gcgtaaacag aacgaaatgc ggcaacgtta
     2101 cggaatacaa caggaaagtc atccatgaaa aacgaacatg aagatcccgg cagttttggc
     2161 gaaacaacga caccggcatc cggcaaatcc ttcctgaaac gccagtatat gggatacagc
//
LOCUS       DAZFLD010000402         2220 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.404, whole genome shotgun sequence.
ACCESSION   DAZFLD010000402 DAZFLD010000000
VERSION     DAZFLD010000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2220
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.404"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..2210
                     /locus_tag="ACHKBL_004918"
     CDS             <1..2210
                     /locus_tag="ACHKBL_004918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001530200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFO5821646.1"
                     /translation="GFDGRTQRYHYDLTGKLTQSEDEGLVILWYYDESDRITHRTVNG
                     EPAEQWQYDGHGWLREISHLSEGHRVAVHYGYDDKGRLTGERQTVENPETGELLWQHE
                     TKQAYNEQGLANRVTPDSLPPVEWLTYGSGYLAGMKLGGTPLLEYTRDRLHRETVRSF
                     GSMAGNTAAYELTSTYTPAGQLQSRHLNSLVFDCDYNWNDNGDLMRISGPRQTREYGY
                     SATGRLESVRTLAPDLDIRIPYATDPAGNRLPDPELHPDSTLTVWPDNRITEDAHYLY
                     RYDEYGRLAEKTDRIPAGVIRTDDERTHHYHYDSQHRLVFYTRIQHGEPLVESRYLYD
                     PLGRRTGKRVWRRGRDLTGWMSLSRKPEETWYGWDGDRLTTVQADTTRIQTVYQPGSF
                     TPLIRIETDVTELEKAQRRSLAEKLQQEGSEDGHGVVFPAELVRLLDRLEEEIRTDRV
                     SSESRAWLAQCGLTVEQLEKQVEPEYTPARKVHLYHCDHRGLPLALISEDGNTAWSGE
                     YDEWGNLLNEENPHHLHQPYRLPGQQHDEESGLYYNRHRYYDPLQGRYITPDPIGLRG
                     GWNMYQYPLNPIQVIDPMGLDAIENMTSGGLIYAVSGVPGLIAANSITNSAYQFGYDM
                     DAIVGGAHNGAADAMRHCYLMCRMTKTFGSTIADVIGKNHEAAGDRQGQPAKERIMDL
                     KNNTVGIACGDFSAKCSDACIEKYNTGQLFGLDGIKADNPIKAKQGSSDASNY"
BASE COUNT          557 a          572 c          707 g          384 t
ORIGIN      
        1 gcggctttga cgggcggacg caacgttatc attatgacct gaccggaaaa ctcacgcaga
       61 gtgaagatga ggggcttgtc atcctctggt actacgatga atcggaccgt atcactcacc
      121 gcacggtgaa cggcgaaccg gcagagcagt ggcagtatga tggccacggc tggctgagag
      181 aaatcagcca cctgagcgaa ggccaccgtg ttgccgtcca ctatggctac gacgataagg
      241 gccgtctgac cggggagcgc cagacggtgg agaacccgga gacgggggaa ctgctgtggc
      301 agcatgagac gaaacaggca tacaacgaac aggggctggc aaaccgcgtc acgccggaca
      361 gcctgccgcc ggtggagtgg ctgacctatg gcagcggtta tctggcgggc atgaagctgg
      421 gcgggacgcc gctgctggag tacacgcgcg accgcctgca ccgggagacg gtgcgcagct
      481 tcggcagcat ggcaggcaat acagccgcat atgaactgac cagcacttac acccccgcag
      541 gccagttaca gagccggcac ctgaacagcc tggtgtttga ttgtgactat aactggaatg
      601 acaacggcga cctgatgcgc atcagcggcc cgcgacagac gcgggaatac ggctacagcg
      661 ccacgggcag gctggagagc gtgcgcaccc tcgcaccgga cctggacatc cgcatcccgt
      721 atgccaccga cccggcgggc aaccggctgc cggacccgga gctgcacccg gacagtacac
      781 tcacagtgtg gccggataac cgaatcaccg aagacgcgca ctacctgtac cgttacgatg
      841 aatacggcag gctggcggag aagacggacc gcatcccggc gggtgtgata cggacggacg
      901 acgagcggac ccaccactac cactacgaca gccagcaccg cctggtgttc tatacgcgga
      961 tacagcatgg cgagccactg gtcgagagcc gctaccttta cgacccgctg ggccgccgga
     1021 cgggtaaacg ggtgtggcgt cgcgggcgtg acctgacagg atggatgtcg ctgtcgcgta
     1081 aaccggagga gacgtggtac ggctgggacg gtgacaggct gacgacggtg caggccgaca
     1141 ccacgcgtat ccagacggta taccagccgg gaagcttcac gccgctcatc cgcatcgaga
     1201 cagacgtgac cgaactggag aaagcgcagc gccgcagcct ggcggagaag ctccagcagg
     1261 aagggagcga ggacggacac ggcgtggtgt tcccggctga actggtgcgg ctgctggaca
     1321 ggctggagga agaaatccgg acagaccgcg tgagcagtga aagccgggcg tggcttgcgc
     1381 agtgcgggct gacggtggag caactggaaa aacaggtgga gccggaatac acaccggcgc
     1441 gaaaagttca tctttaccac tgcgaccacc ggggactgcc gctggcgctt atcagcgaag
     1501 acggcaatac ggcgtggagc ggggagtatg atgagtgggg aaacctgctg aatgaggaaa
     1561 acccgcatca cctgcaccag ccgtaccgtc tgccagggca gcagcatgat gaggagtcgg
     1621 ggctgtacta taaccgtcac cggtactacg atccgttgca ggggcggtat atcaccccgg
     1681 acccgattgg gttgagaggt ggatggaata tgtatcagta tccgttgaat cccatacaag
     1741 tgatagaccc aatggggtta gatgcgattg agaatatgac atcaggtgga ctaatttatg
     1801 ccgtatctgg tgtacctgga ttgattgctg caaacagcat tactaacagt gcttaccagt
     1861 tcggttatga tatggatgct attgttggcg gagctcataa tggggccgcc gatgcaatga
     1921 gacattgtta cttgatgtgt cgaatgacta agacatttgg atcaacaata gctgacgtga
     1981 taggtaaaaa tcatgaggcg gcaggggata gacaaggtca gccagctaaa gaaagaatca
     2041 tggatcttaa aaataacact gtcggtattg cttgtggcga tttttctgcc aaatgtagcg
     2101 atgcatgtat tgaaaaatat aacactgggc aactcttcgg gttagatggt ataaaagcag
     2161 ataatccaat aaaagcaaag caagggagtt cagatgcttc aaattattag aggcaaactt
//
LOCUS       DAZFLD010000403         2185 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.405, whole genome shotgun sequence.
ACCESSION   DAZFLD010000403 DAZFLD010000000
VERSION     DAZFLD010000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2185)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2185
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.405"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..424)
                     /locus_tag="ACHKBL_004919"
     CDS             complement(<1..424)
                     /locus_tag="ACHKBL_004919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HFO5821647.1"
                     /translation="MHVTAKPSSFQCNLKCDYCFYLEKESQFTHEKWMDDSTLKEFIK
                     QYIAASGNQVYFTWQGGEPTLAGLDFFRKVIHYQQRYAGQKRIFNALQTNGILLNNEW
                     CAFLKEHEFLVGISIDGPQELHDRYRRSNSGNGTFAKVI"
     gene            complement(476..2158)
                     /locus_tag="ACHKBL_004920"
     CDS             complement(476..2158)
                     /locus_tag="ACHKBL_004920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310130.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFO5821648.1"
                     /translation="MKSALKKSVVSTSISLILASGMAAFAAHAADDVKLKATKTNVAF
                     SDFTPTEYSTKGKPNIIVLTMDDLGYGQLPFDKGSFDPKTMENREVVDTYKIGIDKAI
                     EAAQKSTPTLLSLMDEGVRFTNGYVAHGVSGPSRAAIMTGRAPARFGVYSNTDAQDGI
                     PLTETFLPELFQNHGYYTAAVGKWHLSKISNVPVPEDKQTRDYHDNFTTYSAEEWQPQ
                     NRGFDYFMGFHAAGTAYYNSPSLFKNRERVPAKGYISDQLTDEAIGVVDRAKTLDQPF
                     MLYLAYNAPHLPNDNPAPEQYQKQFNTGSQTADNYYASVYSVDQGVKRILEKLKKNGQ
                     YDNTIILFTSDNGAVIDGPLPLNGAQKGYKSQTYPGGTHTPMFMWWKGKLQPGNYDKL
                     ISAMDFYPTALDAADISIPKDLKLDGVSLLPWLQDKKQGEPHKNLTWITSYSHWFDEE
                     NIPFWDNYHKFVRHQSDDYPHNPNTEDLSQFSYTVRNNDYSLVYTVENNQLGLYKLTD
                     LQQKDNLAAANPQVVKEMQGVVREFIDNSQPPLSEVNQEKFNNIKKALSEAK"
BASE COUNT          564 a          463 c          494 g          664 t
ORIGIN      
        1 ctatcacttt tgcaaaagta ccgttacctg aattactgcg tctgtaacgg tcatgtagct
       61 cttgggggcc atcgatcgag ataccaacca gaaactcatg ttctttgaga aaggcacacc
      121 attcattatt caataaaatg ccattcgttt gtaatgcatt aaaaatacgt ttttggcctg
      181 catagcgttg ttgatagtga ataactttac ggaaaaaatc caggccagcc agagtgggtt
      241 caccgccttg ccaggtaaaa tagacctgat tgccagacgc tgcgatatat tgtttgatga
      301 actctttcag agtgctgtca tccatccatt tttcatgagt aaactgcgac tctttttcaa
      361 ggtaaaaaca gtaatcacat ttgagattac attgaaaact ggagggcttg gctgtaacgt
      421 gcatcgctat ctcgctcaat aaggcggcgg aaaaatccgc cgcatgaagg tttagttatt
      481 tcgcttcgct tagtgctttc ttgatattgt taaacttctc ctgatttacc tcgctaagcg
      541 gtggctggct gttgtcgata aactctctta ccacgccttg catctcttta acgacctgcg
      601 gattggcggc ggcaaggtta tctttttgct gtagatccgt cagtttgtag agacctaact
      661 gattattttc tactgtatag acaagcgaat aatcgttatt tctcaccgta taagagaatt
      721 ggcttaagtc ctcggtgttg gggttatgcg ggtaatcgtc tgactgatgg cggacaaatt
      781 tgtggtaatt atcccagaat ggaatatttt cctcatcaaa ccagtgagaa taagaggtta
      841 tccaggtcag atttttatgt ggctcgcctt gtttcttatc ttgcaaccag ggcagcaagg
      901 aaacgccatc cagcttaagg tcttttggaa tgctgatatc ggctgcatca agagctgtcg
      961 ggtagaaatc cattgcggaa atcagcttgt cataattacc gggttgaagt tttcctttcc
     1021 accacataaa cattggggtg tgagtaccgc caggataggt ctgactctta tagccttttt
     1081 gcgccccgtt cagcggcaga ggaccatcga taaccgcacc attatcggag gtaaagagaa
     1141 taatagtatt gtcatactgt ccgtttttct tcagtttttc gagaatgcgt tttacaccct
     1201 gatcaacaga ataaacggaa gcgtagtagt tatctgctgt ttgactaccg gtattaaatt
     1261 gcttctgata ttgctccggt gcaggattat catttggcag atgcggagca ttataagcca
     1321 ggtaaagcat aaaaggctgg tcaagtgttt tggcacgatc aacaacgcca attgcctcat
     1381 cggttaactg atcgctgata taaccttttg cggggacgcg ttcacggttt ttgaacagtg
     1441 aaggggagtt gtaatatgcc gttcctgcag cgtggaatcc cataaagtaa tcaaagccac
     1501 ggttttgcgg ttgccattct tccgcagaat aggtggtgaa gttgtcatga tagtcacgcg
     1561 tttgtttatc ttccggtacc ggcacattac tgatttttga caagtgccat ttacctactg
     1621 ctgcagtgta ataaccatga ttctggaata attcaggcag gaaagtttct gttagcggaa
     1681 taccatcctg agcatcggta ttggaataga caccaaagcg ggcgggcgct cgaccggtca
     1741 ttattgcggc gcgggagggg ccggaaacac cgtgcgccac atagccgtta gtaaaacgta
     1801 cgccttcatc cattaatgac aggagcgtcg gcgttgattt ttgcgcagct tcaatggctt
     1861 tatctatccc tattttgtag gtatcgacaa cttcacgatt ttccattgtt tttgggtcaa
     1921 aagatccctt atcaaaagga agttgtccat aaccaagatc atccatagtc agtacgataa
     1981 tatttggctt tcccttggta ctgtattcag tcggcgtaaa gtctgagaaa gcaacgtttg
     2041 ttttggttgc tttcagcttt acatcatctg ccgcatgagc agcaaatgca gccataccag
     2101 atgccagtat caaagatatc gaggtactta cgacactttt ctttaatgca gacttcataa
     2161 atgttcctct tcttattgta cttaa
//
LOCUS       DAZFLD010000404         2160 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.406, whole genome shotgun sequence.
ACCESSION   DAZFLD010000404 DAZFLD010000000
VERSION     DAZFLD010000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2160
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.406"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            282..530
                     /locus_tag="ACHKBL_004921"
     CDS             282..530
                     /locus_tag="ACHKBL_004921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001349431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO5821649.1"
                     /translation="MSTQTGHFYTPSRILRMAELTRLLGISRSTIYEKMNPASKYYDA
                     TFPRPVRLGSSAVGWRASAIDEWLTLHTTPTRSVTKDK"
     gene            546..2111
                     /locus_tag="ACHKBL_004922"
     CDS             546..2111
                     /locus_tag="ACHKBL_004922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3987 domain-containing protein"
                     /protein_id="HFO5821650.1"
                     /translation="MTKLSPPELDITKLPDRCQALLDEMHEETGISREILLSIMLTVK
                     AASVQDTHEVELSGGQRTSLQIYMCLSSASGSGKTSACTKLIAPVHETEEELHQAYID
                     DKKNYDRMMEMWTTDKKILERRYKKEMERSPENAAAARAALEKCIANKPVPPVQQVLI
                     VNDATPEGIALKLSQSPSLLLLSDEGGTILDKRFERKSALYNTLWSGQPVTVERASRP
                     GFRVKDSRLTMLILTQPVIFDKFFTLTGDQIRGNGFLARVLFCEPGANKIMTTEPHTA
                     TPVVTQQCATCFHEKSFGSQIRDSLRASRERRAKGEQRICMTLSNAASHDLEIFHKEN
                     MSAVRQNPRMTTFEDIIVRKREQAVRIAALLELENNPHGTVITRESINSAIYLVDFYF
                     QHLISKLESLREISPAEKLDKWLRENIIRVKGYEYQKSHILQYGPYALRNKCVLDEAL
                     DILAEQKKIVIDYSIGQKIIYIGDAITPCELANEANIPIMERGMFMVCWDHKLNKYRN
                     EEQTKIWNITTKN"
BASE COUNT          696 a          491 c          442 g          531 t
ORIGIN      
        1 gttccagact atacacccct tcagcatcaa taatgtgact gagctcatta taacgttgat
       61 cccagaatct ttgatgcgct ccaaaatcag tactcttccg cttatctctc ggactctctc
      121 cttccttaaa caactcaaaa actgtacaaa tatcatacaa aagatttgta aacatgcgca
      181 tctgctctga tctccggttg gtttccgttt tatcacaggg gacattctgg ccttaaccta
      241 gcaaattaat gacatagcct tcaactcaca tggagaacaa tatgtcaacc cagaccggac
      301 atttttatac cccctcacgc atcctgcgta tggctgaact tacccggctc cttggtatat
      361 caagatcgac catctacgaa aaaatgaatc cggcatcaaa atactacgat gccacctttc
      421 cccggcctgt ccggcttggg agttcggctg taggctggcg ggcatctgcc attgatgagt
      481 ggttaacttt acatacaaca ccaaccagat ccgtaactaa ggataaataa ggaaaactgt
      541 acgccatgac aaaattatcc ccccctgaac ttgacataac taaactgcct gacaggtgcc
      601 aggcattact tgatgaaatg catgaagaaa cgggaataag ccgtgaaata ctgctgtcta
      661 ttatgctgac ggtcaaggct gcctcggtcc aggatacgca tgaagttgaa ctttctggag
      721 ggcagcgtac cagtcttcag atatatatgt gtctttcatc agcatcaggc agtggaaaaa
      781 cctctgcctg cacaaaatta atcgcccctg ttcatgaaac agaagaagaa ctacatcagg
      841 catatatcga cgataaaaaa aactatgatc gcatgatgga aatgtggaca acagataaaa
      901 aaatcctgga acggagatat aaaaaagaaa tggaaagatc cccggaaaat gctgctgctg
      961 cacgagcagc gcttgaaaag tgtatagcaa ataaacccgt acctcccgta caacaggttc
     1021 tcatcgtgaa tgacgcgaca ccagaaggga tagctttgaa actcagccag tcaccttctc
     1081 ttctgttgct gtctgatgaa ggaggaacta ttctggataa gcgtttcgaa cgcaaatcgg
     1141 cgctgtacaa cacattatgg agtgggcaac cagtcaccgt agaacgggca tccagacccg
     1201 ggttccgggt aaaggattcc cgtctcacta tgcttatact cactcaaccg gtaatatttg
     1261 ataagttttt tactctcact ggcgaccaga tccggggcaa tggctttctg gcccgggtgc
     1321 tattttgcga acccggagca aataaaataa tgacgacaga accgcatact gccactcctg
     1381 ttgtaacgca gcaatgtgca acctgttttc atgagaaaag ttttggcagt cagatcagag
     1441 actctctgag agcgtcccgg gaaaggcgtg caaaaggtga gcaacgcatc tgcatgacat
     1501 tatcaaacgc agcatcccac gatctggaaa tctttcacaa agaaaatatg agtgcagtca
     1561 ggcagaaccc acgtatgacc actttcgaag acattatcgt caggaaaaga gaacaggctg
     1621 ttcgaatagc cgcacttctg gagctggaga acaatccaca tggtacagta ataacacgag
     1681 aaagtatcaa tagtgctatt tatctcgttg atttttattt tcagcatctt atatccaaac
     1741 tggaatcact tcgggaaata tctcctgcag aaaaactgga taaatggctc agagaaaata
     1801 tcatccgggt aaaaggatac gaatatcaaa aaagccatat actacaatat ggtccatatg
     1861 cgctcaggaa taaatgtgtt cttgatgaag cacttgatat acttgcagaa cagaaaaaaa
     1921 tcgtaattga ttattcaatc ggccaaaaaa tcatttatat cggggacgcg attacacctt
     1981 gtgaattagc aaatgaggca aacatcccga taatggagcg tggaatgttt atggtctgtt
     2041 gggaccataa gctgaataag tacagaaatg aagaacagac taaaatatgg aacataacta
     2101 caaaaaatta aagttcccgt cttttatcat tcagttacca acctgaatat ctgaaacaat
//
LOCUS       DAZFLD010000405         2158 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.407, whole genome shotgun sequence.
ACCESSION   DAZFLD010000405 DAZFLD010000000
VERSION     DAZFLD010000405.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2158
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.407"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..502)
                     /locus_tag="ACHKBL_004923"
     CDS             complement(<1..502)
                     /locus_tag="ACHKBL_004923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000121930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbA"
                     /protein_id="HFO5821651.1"
                     /translation="MSYQQTSAAEDPMAIWYIVGAICLLFAIIIWRFLPEIVFASCLI
                     LHTLWGMIDWGPFHNFAAPRYNLLAITANNAATITFSQWLDVMSRTVGILWLILLPMT
                     FGFLWMWFHHPAQPRFTRRPLNIHTLPHIFSALSPAIAPVLADGDNNRLFHGQKRPER
                     RVALTPE"
     gene            840..986
                     /locus_tag="ACHKBL_004924"
     CDS             840..986
                     /locus_tag="ACHKBL_004924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001346191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5821652.1"
                     /translation="MSGDLPSHQSIGTMDLCYSKTWPDKQVVLRLVLWPEEVSFQLTM
                     KELL"
     gene            1039..1137
                     /gene="dqlB"
                     /locus_tag="ACHKBL_004925"
     CDS             1039..1137
                     /gene="dqlB"
                     /locus_tag="ACHKBL_004925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001681653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinQ-like type I toxin DqlB"
                     /protein_id="HFO5821653.1"
                     /translation="MINLLIIVLRAVVAVANAVIAILELIRELVDR"
     gene            1723..1818
                     /gene="dqlB"
                     /locus_tag="ACHKBL_004926"
     CDS             1723..1818
                     /gene="dqlB"
                     /locus_tag="ACHKBL_004926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinQ-like type I toxin DqlB"
                     /protein_id="HFO5821654.1"
                     /translation="MINLLIIVLRAVVAVANAVIALLELIRELVD"
     gene            complement(1881..2057)
                     /locus_tag="ACHKBL_004927"
     CDS             complement(1881..2057)
                     /locus_tag="ACHKBL_004927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001054900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5821655.1"
                     /translation="MNLVDAFVTKVISGPYEEYGKWWVDVEYISWGVPGKSRLMFDSR
                     EQAQEVQEGFRFLT"
BASE COUNT          574 a          477 c          569 g          538 t
ORIGIN      
        1 cttcgggggt tagtgcgaca cggcgctcgg gacgtttttg tccgtggaag agtctgttgt
       61 tgtctccgtc agccagaacg ggggcgatcg ccggtgacag agctgaaaaa atatggggca
      121 gtgtgtggat attcagtggc ctgcgggtaa aacggggctg ggccggatgg tgaaaccaca
      181 tccacagaaa gccgaatgtc atgggaagca gaatcaacca gaggatcccc actgtccggg
      241 acataacatc cagccactgg ctgaaagtga tggttgccgc gttgttagca gtgatggcca
      301 gcaggttgta acggggggcg gcaaagttgt gaaagggccc ccagtcaatc atgccccaca
      361 gtgtgtgcag gatcaggcag gaggcgaaga cgatttcagg taaaaagcgc cagatgataa
      421 tcgcaaagag caggcatatg gcaccgacga tataccatat agccattgga tcttcggcgg
      481 cagacgtctg ttggtaagac acggggattc ctccatagat gaatggataa atttcgggat
      541 gaggctgagt ggaattaatg cgaacaatcg ccggtaatga ccgggcaggg ctgaattcag
      601 cagggcattt tcacaggaac agcagaaggt ggtgagagac aggcacaaaa aagccgactg
      661 gtgaggccgg cttttttgcg atcccaggga gagttggtgg ctctggcccg gaggaagtct
      721 cttttcaact aacgttacca gaatatcgtt atattcacct gatcgcaatc tgttcgttac
      781 agtctattgt tccgggagta gcgatatcgc ctgaaacaag tataatcagc aagttagcct
      841 tgtctggaga cttaccttcg catcaatcca tcgggactat ggatctgtgc tattcaaaga
      901 cctggccgga caaacaggtg gtgctgcgtc tggtgctctg gccggaggaa gtctcttttc
      961 agctaacaat gaaagagctg ttgtgacagg caatgtaaga gtaaacaaca gtgatttcct
     1021 ccattgacgg agaaacggat gattaacctg ttaatcatcg ttctcagggc ggttgttgct
     1081 gtggctaacg cagtgatagc aatcctggaa ctgatccggg aactggttga cagataatcc
     1141 cggaaacgta cttaagggca gagggtcacc gccctctgcc ctttaataat ctacggacag
     1201 attactttct gtacttccag agcctgtttc tgtgagtcaa acatgagctg gtttttaccg
     1261 actacaccca aggaaatata ttcgatatct cccatttgcc gtattttcgt aaaattcaac
     1321 aggaaatatt caacgggagt gagagaactg ctgtccgggg aggaggtaca gacgcaaaaa
     1381 aaccgactgg tgaggccggt ttttttgcga ttccggatcg aagttggtag ccctgaccgg
     1441 aagataactg cctataactg actatgaaat aatacggctg aaataatatt tctgcaagca
     1501 cagtataagt atatactcgg tgtgtcagct ttgaggcacg ttctttccgg catatcgttc
     1561 accgggatga acaggttgca agtcctggcc aaaacatcag gtggtgctgg ttttggtgct
     1621 ctggccggag aagaactgtc tataactgac actatagaaa acgtggcgac aggcaatgta
     1681 agggcaaacc acggtgttct ttctccattg atggagaagc ggatgattaa cctgttaatc
     1741 atcgttctca gggcggttgt tgctgtggct aacgcagtga tagctctcct ggaactgatc
     1801 cgggaactgg ttgactgata atcccggaaa cgtacttaag ggcagagggt caccgccttc
     1861 tgccctttaa caaaatatgc tcaggttaaa aatcgaaaac cttcctgcac ttcctgtgcc
     1921 tgttcccgtg agtcaaacat gagccggctt ttacccggaa ctccccagga aatatattct
     1981 acatccaccc accattttcc gtattcttca tacggtccgg atatcacttt agtgacaaat
     2041 gcatcaacca ggttcatttt tacacctttc tgttattctg cttattacga ccagcagaca
     2101 gcaaatatta aaaataacac ggcggtaaaa atggcaattt tttccatgct gtattttt
//
LOCUS       DAZFLD010000406         2141 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.408, whole genome shotgun sequence.
ACCESSION   DAZFLD010000406 DAZFLD010000000
VERSION     DAZFLD010000406.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2141
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.408"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(51..374)
                     /locus_tag="ACHKBL_004928"
     CDS             complement(51..374)
                     /locus_tag="ACHKBL_004928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001350394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5821656.1"
                     /translation="MDCTILLKIISVKEITKNSPLWSHYLARYVDSGYQAEFECEGTA
                     DKWDIYDEWDIYESIADYFDVVGDFNCDVRWSGRKILIKGTCVFPLGEPVKGIHNIPL
                     WIDVE"
     gene            complement(407..1309)
                     /locus_tag="ACHKBL_004929"
     CDS             complement(407..1309)
                     /locus_tag="ACHKBL_004929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase domain-containing protein"
                     /protein_id="HFO5821657.1"
                     /translation="MSRREKLLKQDLVAHIRKDKGSFKKLSDYTRIMHRFAESLAKMN
                     VQISSAAQVKVRHIELYMRSRSNVSGRTRENEMSAIRVMLRQAGKYKMADPEHPRLSN
                     KALGISGSSRKGTKKLITDERFREILQRVEAKDKGVAAVVKLSRYLGLRNEEAIQSAK
                     SLRTWKKALQRGDEKIRVIFGTKGGRERMTTVVDRDNVLDAVNYALKYASEHNGRLID
                     RDNLKSAMDYYLNTLRRYGELKGGDETAHSFRYSYAASAMKYHIMNGYSYKEALALTS
                     IDLGHGDGRGRYIEQVYCQLLGYE"
BASE COUNT          610 a          535 c          385 g          611 t
ORIGIN      
        1 agcgacatat aagcctgtat atttttaaat cacaaaagat gaatgtcaag ttattcaaca
       61 tcaatccaaa gaggaatatt atgtattccc ttaacgggtt ctcccagagg aaaaacacag
      121 gtccctttaa tcagtatttt tcttcccgac catctgacat cacagttaaa atctcccacc
      181 acatcaaaat aatccgcaat ggattcatag atgtcccatt catcatagat atcccattta
      241 tctgctgttc cttcacattc aaactcggcc tgatatccgg aatcaacata tcgcgccaga
      301 tagtgtgacc acaaaggcga attttttgta atctctttta cagagattat tttcagcaaa
      361 atagtacaat ccataatatt tacccgtacg aaatcgccat gagttattac tcatagccta
      421 acaactgaca ataaacctgt tcaatatatc gtcctctgcc atcaccatga ccaagatcaa
      481 ttgatgtcag tgcaagtgcc tccttataac tgtaaccgtt cataatatga tatttcatgg
      541 cacttgcagc gtaactgtac cgaaaactgt gtgcagtctc atctccgcct ttcagttctc
      601 cataacgacg taatgtatta agataataat ccattgccga cttaagatta tctctgtcta
      661 taagacgtcc attatgttca ctggcatatt tgagtgcata attaacagca tcaagaacgt
      721 tatcccggtc aacaaccgtt gtcattcttt cccgtccccc tttcgtacca aaaatcactc
      781 ttattttttc atctccccgt tgtaacgctt ttttccaagt tcgtaatgat ttggccgact
      841 ggatagcttc ttcatttctc agaccgaggt aacgtgacag tttaacaaca gcagccaccc
      901 ctttatcttt tgcttccaca cgctgaagaa tctcccgaaa acgctcatcc gtgattaatt
      961 tttttgttcc cttacgactg gagccggaaa tgcctaacgc tttattgctg agacgaggat
     1021 gttcgggatc agccatctta tatttacctg cctgacgtaa cataacccgt attgcagaca
     1081 tttcattttc ccttgttcgc ccagaaacat tactccggct tctcatatat agctcaatat
     1141 gccggacttt tacctgcgcg gcagaactga tctgtacatt catttttgcc agactttcag
     1201 caaagcggtg catgatcctg gtgtaatcac tcagcttctt gaagctgcct ttgtcttttc
     1261 gaatgtgagc aaccagatcc tgtttcagta atttttcacg tctggacatc aacttctcct
     1321 gcacataata atttttacct taaacagctg tacgttcggc agatgagttt ttctgttgtg
     1381 ccgtgaacac aacagaaaaa ctcatctgca tttctccctg acgacacggt taaacgttgc
     1441 gatccttcgg gtagtgactg cctgtgcgcc agggtagcgc agattgttta ggtattgagg
     1501 ggtaacaaat cggtgttgca caccgactgc agcatttgct gtacctgtct ccaggtcgcc
     1561 cccgacacca cctctaataa gtggtatcgc cgattcggca tctcactaca taaaactcct
     1621 taaaaaacaa ttgattctgc aaaaaaacat ttatggacgt tattcacaaa atcgtccgta
     1681 aatttgcaga agaggttgaa aaaacactct cacgccgggt cacttcttca aatgttaaaa
     1741 aacatcacgt gaaacggcag ttcgtgcggg ttcgttaagc agacgcgcgt gcgtcacttt
     1801 gtcatcgtga cacgctgacg aaagtgacgc cgcgcagctt cagacgccag atacacgcgt
     1861 aagcgaccaa atttcaaatg gtcttataca gcgcgcacag tcgcaggaag tgctggcaaa
     1921 caaatgaaaa gaataaaaga agtcaatacc gccattacgc ggtaagaaca ttccggcatc
     1981 accggataga taaggatcac acaaccactg ctgtgtattg cccgtaataa agggcgcggt
     2041 taaaagaaac cttcatcatc atgactgacg atatctgctc tcaaaggtgg gcagatttac
     2101 caccaccaga aacatcctag ctgcacatcc cctttcaggt t
//
LOCUS       DAZFLD010000407         2139 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.409, whole genome shotgun sequence.
ACCESSION   DAZFLD010000407 DAZFLD010000000
VERSION     DAZFLD010000407.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2139)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2139)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2139
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.409"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..222)
                     /locus_tag="ACHKBL_004930"
     CDS             complement(<1..222)
                     /locus_tag="ACHKBL_004930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001293190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFO5821658.1"
                     /translation="MSELSYLEKLLDGAEVEWKTLGKVLKRTKGTKITAGQMKALHKD
                     NAPLKIFAGGKTVAFVDFKDIPEKDINREP"
     gene            complement(219..1775)
                     /locus_tag="ACHKBL_004931"
     CDS             complement(219..1775)
                     /locus_tag="ACHKBL_004931"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HFO5821659.1"
                     /translation="MTSIQQRAELHRQIWQIANDVRGSVDGWDFKQYVLGALFYRFIS
                     ENFSGYMEAGDDSICYAALDDGIITDDIKDDAIRTKGYFIYPSQLFCNVAAKANTNDR
                     LNADLNSIFVAIESSAYGYPSEADIKGLFADFDTTSNRLGNTVKDKNARLAAVLKGVE
                     GLNLGDFNKHQIDLFGDAYEFLISNYAANAGKSGGEFFTPQHVSKLIAQLAMHGQTHV
                     NKIYDPAAGSGSLLLQAKKQFDDHIIEEGFFGQEINHTTYNLARMNMFLHNINYDKFD
                     IKLGNTLTEPHFRDEKPFDAIVSNPPYSVKWTGSDDPTLINDERFAPAGVLAPKSKAD
                     FAFVLHALNYLSARGRAAIVCFPGIFYRGGAEQKIRQYLVDNNYVETVISLAPNLFFG
                     TTIAVNILVLSKHKTDTRVQFIDASELFKKEANNNILTDAHIEQIMQVFASKEDVAHL
                     AKSVAFETVVANDYNLSVSSYVEAKDTREIIDIAELNAELKTTVSKIDQLRKDIDAIV
                     AEIEGCEVQK"
     gene            2041..>2139
                     /locus_tag="ACHKBL_004932"
     CDS             2041..>2139
                     /locus_tag="ACHKBL_004932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HFO5821660.1"
                     /translation="MNPSDAIEAIEKPLSSLPYSLSRHILEHLRKLT"
BASE COUNT          536 a          525 c          467 g          611 t
ORIGIN      
        1 tggctctcta ttaatatctt tctctggtat atctttaaag tcaacaaagg ctactgtttt
       61 tcctcctgca aaaattttaa gcggcgcatt atctttgtga agagctttca tctgcccagc
      121 ggttattttt gtgcctttag ttcgtttaag cactttccct agtgttttcc actcaacctc
      181 agccccatcc agcaattttt ccagataact caactcgctc atttctgcac ctcgcagcct
      241 tcaatttcag ccacaatcgc atcaatatct ttacgcaact ggtcgatttt gctgaccgtg
      301 gttttcagct cagcattcag ctcagcaata tcgataattt cacgagtatc tttcgcttcc
      361 acatagctgc tcaccgacag gttatagtca ttagcgacaa cggtctcaaa cgcgactgat
      421 ttcgccagat gggcaacatc ttccttgctg gcaaatacct gcataatctg ttcgatatga
      481 gcatcggtca gaatgttgtt gttagcctct tttttgaaca gttcgctggc atcaataaac
      541 tgaactctgg tatccgtttt atgtttagac agcaccagta tgtttacggc aatggtggtg
      601 ccaaagaaca ggttcggtgc cagtgaaatc acggtttcga catagttatt gtcgaccaga
      661 tactgacgga ttttctgctc tgcgccgcca cggtaaaaaa tacccgggaa gcagacaatc
      721 gcagcgcgac ctctggccga aagatagttc agcgcatgta atacaaacgc aaagtcagct
      781 ttggatttgg gggccagaac gccagccggg gcaaaacgct catcgttaat cagtgtcggg
      841 tcatcgctgc cagtccattt caccgaatac ggcgggttag aaacgatggc atcaaacggt
      901 ttttcatctc tgaagtgcgg ctcagtcagc gtattgccca gcttgatatc aaacttgtcg
      961 tagttgatgt tgtgcaaaaa catgttcata cgcgccaggt tataggtcgt atggttgatt
     1021 tcctgaccaa aaaagccttc ttcgatgata tggtcatcaa actgcttttt cgcctgcaac
     1081 aacagcgaac cggaacccgc tgccgggtcg tagattttgt taacgtgggt ctgcccgtgc
     1141 atagccagtt gtgcaatcag cttagagacg tgctgtggtg taaagaactc gccgcctgac
     1201 ttaccggcat ttgcggcata gttagaaata aggaattcat aggcatcgcc aaacaggtca
     1261 atctgatgtt tgttgaagtc accaaggttt aacccttcaa cccctttcag aacagcagcc
     1321 aggcgggcat ttttatcctt aacggtgtta cccaggcggt tactggtggt atcgaaatca
     1381 gcaaacaaac ccttgatgtc agcttctgaa ggataaccgt aagcagaact ttcgatagca
     1441 acgaagatgc tgtttaaatc tgcattcagt ctgtcattgg tatttgcttt cgcagctacg
     1501 ttgcagaaaa gctgactggg gtagatgaag tagcctttag tcctgatggc atcgtcttta
     1561 atgtcatcag taataatgcc gtcatcaagt gccgcataac agatgctgtc atcaccggct
     1621 tccatatacc cggagaaatt ttcgctgata aaacggtaga aaagcgcgcc cagaacgtat
     1681 tgcttaaaat cccatccatc gaccgaacct ctgacatcgt tagcaatttg ccatatctgg
     1741 cggtgcagtt ctgctctttg ttgaatactt gtcattttta acctgttttt cctggtctgg
     1801 aactgaatct ccgggggcaa tactacagca atcttgtggt tcaggcagcc ccggagcaag
     1861 tcagcagata ttgtgagata caccaggtta ttcatgacag ggaggaggaa cggaacactg
     1921 cctttctttt acagtgcacc gggtacattc aaaataattt cagacagata ttatcgttgt
     1981 gagtcccccg ccacctttcc ggtgcgcggg gttttgtctt ttttcaccgg gaatacatgt
     2041 atgaatccgt ctgatgccat tgaggcaatt gaaaaaccgc tctcctccct gccttactca
     2101 ctttcccgtc acatcctgga acatctgcgc aaactcacc
//
LOCUS       DAZFLD010000408         2129 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.410, whole genome shotgun sequence.
ACCESSION   DAZFLD010000408 DAZFLD010000000
VERSION     DAZFLD010000408.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2129)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2129)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2129
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.410"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..209
                     /locus_tag="ACHKBL_004933"
     CDS             <1..209
                     /locus_tag="ACHKBL_004933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001604127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5821661.1"
                     /translation="VYIGSDTCHITITCNQSGFHLTRDGQRVFTERYIRNLNELLPVL
                     KRRWDVTPAIIRAVEYLSRVPVSH"
     gene            264..578
                     /locus_tag="ACHKBL_004934"
                     /pseudo
     CDS             264..578
                     /locus_tag="ACHKBL_004934"
                     /inference="COORDINATES: protein motif:HMM:NF018516.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ImpA family N-terminal
                     domain-containing protein"
     gene            903..1052
                     /locus_tag="ACHKBL_004935"
                     /pseudo
     CDS             903..1052
                     /locus_tag="ACHKBL_004935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309409.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1227..1628
                     /locus_tag="ACHKBL_004936"
                     /pseudo
     CDS             1227..1628
                     /locus_tag="ACHKBL_004936"
                     /inference="COORDINATES: protein motif:HMM:NF023902.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VasL domain-containing protein"
     gene            1610..1834
                     /locus_tag="ACHKBL_004937"
                     /pseudo
     CDS             1610..1834
                     /locus_tag="ACHKBL_004937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309413.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
     gene            1837..2123
                     /locus_tag="ACHKBL_004938"
                     /pseudo
     CDS             1837..2123
                     /locus_tag="ACHKBL_004938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005687610.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT          551 a          524 c          566 g          488 t
ORIGIN      
        1 ccgtatatat tggcagcgat acctgtcata tcaccattac ctgtaatcag tccggttttc
       61 atttaacccg ggacggacaa cgggtattca ctgagcgtta tatcagaaac cttaatgagc
      121 tgttaccagt tctgaaacgg cggtgggatg tgacaccggc aatcatccgt gcagtggaat
      181 acctttcccg ggtcccggta tcacactgac attctttccg taactgttaa tcatgattta
      241 atacagcaac actgaatctg ccgatgactt ctgaagtgaa actgaaaaca ggtggtgacc
      301 cccggtcatt cccggattat gctgccctgc gcgatgaaat cagcaaactg acccacccgg
      361 cacgcccgga tgtggactgg cgttatgtgg aaacgctctg cctgcgcctg tatgaacaca
      421 atggcgtgga attacagaca gcatcctggt acaccattgc ccggatgcat acaacagggt
      481 taagtggcct gaatgaaggg ctggctctga ttgtggcgct gacccggcat cactggtcag
      541 tgatgtggcc gctgaatacc cacgcccggc tggaaattat taccggtctg ttcaaccgtc
      601 tgcaaaaaac gctccgggcc atgccaccgg acgaccgtga caacctgccc ctgctgtatc
      661 agaccgaaac attcctgaag gcactgagtg atacactggc ccggcacgaa cttaaacaga
      721 gcagtaaagt ggctttaccg gaaaccatgg tgaagggata tatcacccgt ctggaaaatc
      781 agccgatgca gggcgaagtg tcgtctccgg taacccttcc tgctcgggca ctccggtctg
      841 atgctccgga tgtacaggga catcagcccc tgccgcactc ccgtctggtg tatattgttt
      901 ctgaaacaaa agcagagccc acaccctccc ttcaaaaatc cccgcccacg tttctgaaac
      961 cgtttgtggc tggtgtctgt gcggcattgc tggcggtcag cgtggccatt ccgggatggc
     1021 agtttctgac acagccatca ccggcggagc agcaactcag ggcgctgctg gcaccactac
     1081 cggatatgct ttcagcagac cagatggcac agctcagcag aacaccctca ctgttaaatc
     1141 atgcgtcaga ctggataaca ctttcaggtc agcagataac gcgactgaca gaactaccgc
     1201 tgacatacaa cctgcagcgt agtgcgcagc tgttgcagca actgatggta cttttccccg
     1261 ataatcctcg tgtacaggaa atggtggata actggcagaa gagcgtaagg agtcgtgcgc
     1321 tcccggaaga ggcgatgacg ggctggaatg aaggcatgac ccgcttacag cagctggctg
     1381 aacgcctgaa ccgcctggat gaacagcggg gaaaatacat gacggtcagt gaactgaaaa
     1441 cggaggtgtt tggcatcatg caggctttta accggcacat accggcagaa gagcagttac
     1501 gtcgctatga tgaagtccgt aatcagaatg gcagcgaatc tcagcagaaa ctggcgcagg
     1561 atgctctgat ggaacagttg aaccgctact ggcttctgag acatggagat gctgggaatc
     1621 cggcgtgact tgggttcgcc aaccaccgga agacgccatt acggatatgg cgtattttcc
     1681 cttttaccgg tatgccatgg cctttgaagg attcattccg gcattttcat cttggtttgc
     1741 aggtaaccgg tgtggagtgg caatactgac cggtgtccgg gcggatgaat ccctcaactg
     1801 ctttatgggg ctggtgtctc agcgcaaact gagagaatta cgtgggtagg atcagatgat
     1861 ggtattggaa ctgaaggtga tatgggaaaa tggttgttat tattttgtct gctgtagctc
     1921 agtctgaatg acaacatata cttgaaagaa caaatgaagg ccgtctcgaa gccgaagtaa
     1981 agggtattta atttgggcga aaaagaacgg tcaacaggga acaagtacaa accttacgaa
     2041 gtctgggtat cggcgctaca gaaatttcgc gaaagatgaa atttacacgt tctactgtgt
     2101 ataaaatatt agagggtaaa taatatttt
//
LOCUS       DAZFLD010000409         2118 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.411, whole genome shotgun sequence.
ACCESSION   DAZFLD010000409 DAZFLD010000000
VERSION     DAZFLD010000409.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2118)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2118
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.411"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..2047
                     /locus_tag="ACHKBL_004939"
     CDS             <1..2047
                     /locus_tag="ACHKBL_004939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001340207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HFO5821662.1"
                     /translation="KSRKSVKSVEPDIADRANSWLKSYGLDYKLEQAPLNNEIDNALD
                     AYFSKNGGAGGNRPDVKLLVQDKYGKQYPVLIEYKGYKDRLIRLGSNGIIENKDARKE
                     WNFKNINGYAVNGAVHYANALLQFTNYPDIIAIGMTGWRDDDTGELHHEIGVWYVSKN
                     NLGAGQKVGEFTDLSFLADKNVDGFLNKIKLLNLPPEELEKIKASKEEEIDTRLSRLN
                     NDIYQNEKGLGESDRVYLVVATVIATLGIPGKLAPLDKKELTSSTEEDLRDGDIIFRK
                     IRNFLRLKAVPETKREMILRSLQNTLWTENINKPVNGESQLKRVFVKVVDDLGEYYKI
                     GLTTDFTGKLFNEMYRWLGFTQDKLNDVVLTPPYVATLLARLARVNKDSYVWDFATGS
                     AGLLVAAMNEMLIDARENIHSPNELQLKEAQIKAEQLLGLEVLSSIYMLAILNMILMG
                     DGSSNILNKDSLADFDGKYGFGKTGEKFPADAFILNPPYSAKGNGMIFVQKALSMMDK
                     GYAAVIIQSSAGTGKATEYNKKILKENTLLASIKMPADLFIGKSSVQTYIYVFQVKIP
                     HNAKQAVKFIDFSNDGYARSNRKKARNNLVDADRAKERYQEVVDLVHFGKGCLNIFTE
                     DEYFEGTIDPDSGEDWNQTRPVDTRPTLEDFKKTVGDYLAWEVSKLLQGRDASEKKQ"
BASE COUNT          650 a          406 c          520 g          542 t
ORIGIN      
        1 taaatcaaga aagtctgtaa aatctgttga accagacatt gccgaccggg ctaacagctg
       61 gctcaaatct tatggacttg attacaaact ggagcaggca ccacttaaca acgagatcga
      121 taacgcactg gatgcttatt tttcgaaaaa tggcggcgca ggcggtaatc gtcccgacgt
      181 aaaactcctt gttcaggaca aatacggcaa gcagtatccc gttctcatcg agtataaagg
      241 ctacaaggac aggcttatca gacttggtag caacggtatt attgagaaca aagatgccag
      301 aaaagaatgg aattttaaaa atattaatgg atacgcggtg aatggcgcag tccattatgc
      361 caacgcactt cttcagttca ccaattaccc tgatattatt gctattggta tgaccggctg
      421 gcgcgatgat gatacaggcg aactgcatca tgaaatcggc gtgtggtacg tatcaaagaa
      481 taatttagga gccgggcaga aggttggtga attcaccgac ttatcattcc ttgctgacaa
      541 aaatgttgat ggatttctca acaagataaa gctgcttaat cttcctcccg aagaactgga
      601 aaaaatcaag gccagcaaag aggaagagat agacacgcga ctttcccgtc tgaataacga
      661 tatttaccag aatgaaaagg ggcttggtga aagcgatcgt gtttaccttg ttgtggctac
      721 cgttattgca acgttgggga ttccgggaaa gttagcaccg ctggataaaa aagaactgac
      781 ctcttcaacg gaggaggact tgcgtgatgg tgatattatt ttcaggaaaa taaggaattt
      841 tttaaggctg aaagctgttc ctgaaacaaa aagagaaatg attttgcgct cgttgcaaaa
      901 tacgctgtgg actgaaaaca ttaacaaacc agtcaacggt gaaagccagt taaagcgcgt
      961 gtttgttaaa gttgttgatg atcttgggga gtattacaaa atcggactga ccaccgattt
     1021 tacgggcaaa ctgtttaatg agatgtatcg ctggcttgga tttacgcagg acaaactcaa
     1081 tgacgtcgtt ctgacaccac cttatgttgc cacattgctg gcacgactcg ccagagtaaa
     1141 caaagattcc tatgtgtggg attttgctac aggttctgcg ggattgcttg tggctgcaat
     1201 gaatgaaatg ctcattgacg ccagagaaaa tattcactca ccgaatgaac tacaacttaa
     1261 ggaagcgcaa atcaaggctg aacagcttct tggccttgag gtgttatcca gtatttatat
     1321 gctggccatc ctgaatatga ttttgatggg ggatggtagc tcgaatatcc tcaataaaga
     1381 ctcgctggcc gattttgacg gcaagtatgg atttgggaaa acaggagaaa aattccctgc
     1441 ggacgcattt attctcaatc ctccgtattc agctaaaggt aacggcatga ttttcgtgca
     1501 gaaggcgctg tcgatgatgg ataaaggcta tgctgcagtg attatccaga gttcagccgg
     1561 cacaggaaag gctacggagt acaacaaaaa aatactcaag gaaaatacct tgctggcaag
     1621 catcaaaatg cctgcggatc tgtttattgg taaatcaagc gttcagacgt acatttacgt
     1681 ttttcaggta aaaattccgc ataacgcgaa acaggctgtt aagtttatcg acttttccaa
     1741 tgacggctat gctcgctcta atcgcaaaaa ggcaagaaac aatcttgttg atgccgatcg
     1801 tgcaaaagag cgttatcagg aggttgtgga tctggttcac ttcgggaaag gttgccttaa
     1861 tatctttacg gaggatgaat attttgaggg aacaatcgat cctgatagtg gtgaggactg
     1921 gaatcagacc aggccagtag ataccagacc gacactggag gattttaaaa agacggttgg
     1981 cgactatctt gcgtgggagg tgtcaaaact tcttcagggg cgtgatgcgt cagaaaaaaa
     2041 gcagtaaact cccgtcttgc agagcttgag cgggagttta ttaaatctgg tggtaaatgg
     2101 gaagaataca caatcgaa
//
LOCUS       DAZFLD010000410         2115 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.412, whole genome shotgun sequence.
ACCESSION   DAZFLD010000410 DAZFLD010000000
VERSION     DAZFLD010000410.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2115)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2115)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2115
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.412"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..754
                     /gene="dcuC"
                     /locus_tag="ACHKBL_004940"
     CDS             <1..754
                     /gene="dcuC"
                     /locus_tag="ACHKBL_004940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005284671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DcuC"
                     /protein_id="HFO5821663.1"
                     /translation="VVFHTPTTITCGVICAVILTVVAIVRKETKCDSAGNISEQTQVE
                     FTDRDICFYLKSVAPFLPVALLMLAEFGFLGETKITVPAAMILGCLYALIVSKSNPKE
                     LVSSFFEGMGTSYANILGIIISAAVFIEGLRSVGIIDVFIRFLTDSPEFARWGGTLGP
                     FLMGLITGTGDATAFAFNEVVTPHANNFGYDTNTLGAAVMLASALGRNMSPLAGATII
                     CAGLAGVNPVEIAKRTAPGMIVTVLFIALLFL"
     gene            767..2077
                     /locus_tag="ACHKBL_004941"
     CDS             767..2077
                     /locus_tag="ACHKBL_004941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004248927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HFO5821664.1"
                     /translation="MSELNQLQKWRREFHHIPESGWCEFITTSKIITILRGMGWQVIT
                     GNKYLSTEYIMGRNATEVADSIKRAVDSGVPHELLDEMDGFTGCVALWDTGKNGAVTA
                     FRFDIDCVQVSESKSKDHIPEKMGFASIHSGLMHACGHDGHISIGLGLAQWVSENNHK
                     LCGKIKLIFQAAEEGVRGAKPIAEGGILDDCDYFLSSHIGMGVPTGNVVASPTDLLCT
                     TKMDFFFYGTPAHAGVNPHLGNNALAAACHCATQLLAIPRHGEGMSRINIGTLNAGEG
                     RNIIPSFARMQLEVRGETETINQYMREHALKIAQGISSIFGLRFEYKVVGEATDLHND
                     PLLVNLIHYIAQEDMNMSLTEKSFGGSEDATLLIRRVQKIGGKAAYIILGSDLKAGHH
                     EVDFDFDENVLDTGVKLFSLCLERLNSPGLNKIVGNGKLESELY"
BASE COUNT          599 a          380 c          494 g          642 t
ORIGIN      
        1 cgtcgtattc catactccga ctacaataac atgtggtgta atatgtgctg ttattcttac
       61 agttgttgct attgtaagaa aagaaacaaa atgtgattct gcaggaaaca tatcagaaca
      121 aacacaggtt gagttcactg accgtgatat ctgtttttat ctgaaatctg tagcaccatt
      181 tcttcccgta gcattactta tgctggctga atttggtttt cttggagaga ctaaaataac
      241 cgttccagcc gcaatgattc tcggttgtct ttatgccctg attgtcagta aatccaatcc
      301 caaagaatta gtttccagct tctttgaggg tatggggaca tcatatgcaa atattcttgg
      361 gatcataatt tccgcagctg ttttcattga aggtttaaga tctgttggta tcattgatgt
      421 gtttatcaga tttcttactg actcaccaga atttgcccgc tggggcggta cattggggcc
      481 attcctgatg ggacttataa ccggcacagg ggatgcgact gcattcgcat tcaacgaagt
      541 tgtcacacca catgcgaaca attttggtta cgatacaaat actctgggtg ctgcagttat
      601 gctggcttct gcgctaggga gaaacatgtc tccgttggct ggtgcaacta ttatctgtgc
      661 cggtctggct ggagttaatc cagttgaaat tgcaaaacgc actgcccccg gaatgattgt
      721 gacggtattg tttattgcat tattattcct ttaacaggag gcgtatatgt ctgaactgaa
      781 tcagttacaa aaatggcgga gagaatttca ccatattccg gaaagtgggt ggtgcgaatt
      841 tatcacaacg tcaaagataa ttactattct gcgtggtatg ggatggcagg ttattacggg
      901 gaataaatac ctgtcaacag agtatattat gggccgtaat gcaactgaag ttgcagatag
      961 tataaaacgt gcagttgatt caggcgttcc ccatgaattg ctggatgaaa tggatggctt
     1021 tacagggtgt gttgctttat gggatacagg aaaaaatgga gctgtcactg cgtttcgttt
     1081 tgacattgat tgtgtacagg taagtgaatc aaaatcgaaa gatcatattc ctgaaaaaat
     1141 gggatttgca tccatacact ctggcttaat gcatgcatgt ggacatgatg gtcatatatc
     1201 gattggttta ggattggcac aatgggtatc tgaaaataac cataagttat gcgggaaaat
     1261 taagctaatt tttcaggctg cagaagaagg cgttcgcggt gcaaaaccta ttgcagaagg
     1321 tggtattctt gatgactgcg actacttcct ttcttcacat attggtatgg gagttcctac
     1381 aggaaatgtt gtcgcttcac ctacagattt gttgtgtacg acaaaaatgg atttcttttt
     1441 ctatggtact ccggcacatg ctggtgtaaa tcctcacctg ggaaataatg ctctggcagc
     1501 tgcttgtcac tgtgcaacgc aattacttgc cattccacgc catggtgaag gtatgtcccg
     1561 gataaatatt ggcacgctaa acgcaggtga aggaaggaac ataatcccgt cttttgcaag
     1621 gatgcaactt gaagttcgtg gagaaacgga aacaataaat caatacatgc gtgaacatgc
     1681 tctgaaaata gcacaaggta tttcttccat atttggttta cgttttgagt acaaagtggt
     1741 tggtgaagca acggatttac ataatgaccc tttacttgtc aatctgatac actatatagc
     1801 acaagaagat atgaacatgt ctttgacaga gaaatcattt gggggcagtg aagatgcaac
     1861 gctccttatt cgccgtgtac agaagatagg aggaaaagca gcatatataa tacttggcag
     1921 tgatttaaaa gctggtcatc acgaagttga ttttgatttt gatgagaatg tgctggatac
     1981 aggcgttaaa ctgttttcgt tatgtctgga gagattgaac tctcccggac taaataaaat
     2041 tgtagggaac ggaaaactgg aatctgaatt gtattgattg ctgacaggta tcctgtcaat
     2101 cactcctgat ttctg
//
LOCUS       DAZFLD010000411         2031 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.413, whole genome shotgun sequence.
ACCESSION   DAZFLD010000411 DAZFLD010000000
VERSION     DAZFLD010000411.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2031
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.413"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..216)
                     /locus_tag="ACHKBL_004942"
     CDS             complement(<1..216)
                     /locus_tag="ACHKBL_004942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFO5821665.1"
                     /translation="MHTRIDYLADKYCFTELNESSRLRLQWQDVLEECRQTEAGPEER
                     LRIALLNVDYVTSFELPFRLLLTRTPQL"
     gene            complement(305..814)
                     /locus_tag="ACHKBL_004943"
     CDS             complement(305..814)
                     /locus_tag="ACHKBL_004943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5821666.1"
                     /translation="MQITEALLSEPGEIRRFVQQAVDHWPHLLAIHFTLHSTEGVCGQ
                     QIQTFCSSFHRRVHERITERDHTVSPSAPVVLRWLREQHEGAPIRCLLLLSQTSVCHP
                     RASVTVDEACSQVADLLQQTWPVISAGGQCRVERCFRVVRPDTSEQYVALKTAVQSLM
                     PRGIATIIR"
     gene            complement(1095..1273)
                     /locus_tag="ACHKBL_004944"
                     /pseudo
     CDS             complement(1095..1273)
                     /locus_tag="ACHKBL_004944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842627.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
     gene            complement(1341..1611)
                     /locus_tag="ACHKBL_004945"
                     /pseudo
     CDS             complement(1341..1611)
                     /locus_tag="ACHKBL_004945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001593693.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1661..>2031)
                     /locus_tag="ACHKBL_004946"
     CDS             complement(1661..>2031)
                     /locus_tag="ACHKBL_004946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HFO5821667.1"
                     /translation="EYLQCKRRKDQSVEPTPLYYLWSREAVANDTTPYLMVLLLNNDV
                     WCKSREVVVLRNLIEQAWGKVLGEDNSITHLVVYPEVLSLVFDAANTYNAEPGVLEQT
                     ARAYIESHGTIERHLGCSDF"
BASE COUNT          530 a          526 c          483 g          492 t
ORIGIN      
        1 cagttgtggt gtacgggtaa gcaacaagcg aaaaggcagt tcaaaactgg tgacgtaatc
       61 cacattcagc agggcaatgc gaagtcgttc ttctggtccg gcttctgtct gccggcactc
      121 ctccaggaca tcctgccact gcaggcgaag acgggaagac tcattcagtt ctgtaaagca
      181 gtatttatcc gccagatagt caattcgtgt atgcatactg aagagtattc cgtataaaga
      241 ttcagctggc aaaactttat cagtctgtaa aaactaacgg aagagtcgat atttctcccg
      301 tccatcaccg gatgattgtt gcaatacccc gtggcatcag agactgaaca gccgttttta
      361 acgcaacgta ttgctctgat gtatcaggcc ggacaacccg aaaacacctt tccacccggc
      421 attgtccgcc agcgcttatc accggccagg tctgttgcag taaatccgcc acttgcgaac
      481 atgcttcatc aactgtgaca ctggcccgcg gatggcaaac actcgtctgg ctgagcagca
      541 acagacatcg tatcggtgct ccttcatgtt gttcccgcaa ccagcgtaat accaccggcg
      601 ctgatgggct gaccgtgtga tcacgctccg taatacgttc gtgcacccgt cggtgaaagg
      661 aactacagaa tgtctgaatc tgttgaccac agacgccttc tgtcgaatga agtgtgaagt
      721 ggattgccag cagatgcggc cagtgatcca ccgcctgctg aacaaaacgc cggatttccc
      781 ccggctctga aagtaaggct tcggttattt gcactatttt atctctgttg aacttggtta
      841 agtcggtgca gacgcatcaa cacaagtacg gttcgatgca aacagctgtg actggcaata
      901 tgaaaggaat gatgaatcag tcaggatgac aaagtgccgg ctgaccggag gggacgcagg
      961 aagattcacg gggggaccag caccagggaa cagcgccaca ataccaacgc tgacacgttg
     1021 aacattgcca gcgtaccggt atcacaacac gtttcatact tctgccaccg tgattcttcg
     1081 attcgttact gtatctactg tgacacttcg cttttatacc tgcggctgga tcggcccggc
     1141 ttgatgaatc ttcactgatc agcttataaa accctctgtc ggtcataccg gtgaaactgg
     1201 tgatatagtt catgtcaatc agggaattat cggcacgcag aaatacgctg tcgtggcttg
     1261 ttgtagtcaa catggtcaga atgtcctctg tgagatttat gaagattgtg cgaatgcggg
     1321 gaatctgctg agctgtgctt tcagaactgg cctgttacgg gataccaggg attaccggcg
     1381 gggtaacggg cttccggatc atacacacca cgattatcgc ggacaaaatc actgaacgcc
     1441 catatcacct ctttaagtat gtcttcgcag tccggtacat gacgatccag cgccacatcc
     1501 cgagtggtac tgctttgatg cgcccggtga cacaaagccc ggattgttcc agacatcctg
     1561 aatcaaacgc cccagattag gggcgtcgaa atatgcctct ctgaccatta tattccggcg
     1621 tacaggtagc aggtcagaag tgacaatgcg tcacctgacg ttaaaagtca ctacacccaa
     1681 gatgacgttc aatggtacca tgtgattcaa tgtaagcccg ggccgtctgt tccagtacac
     1741 caggctcagc gttgtatgtg ttagctgcat caaataccaa cgacagcact tcaggataca
     1801 caaccagatg tgtaatggag ttatcttcac ccaatacttt tccccacgcc tgctcaatca
     1861 gatttctgag aaccaccacc tcacgactct tacaccagac atcgttatta agtagcagca
     1921 ccataagata aggagtggta tcgttagcca cagcttccct actccagaga taatataaag
     1981 gggtgggctc aacagattga tctttacgtc gcttacactg caaatattca g
//
LOCUS       DAZFLD010000412         2015 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.414, whole genome shotgun sequence.
ACCESSION   DAZFLD010000412 DAZFLD010000000
VERSION     DAZFLD010000412.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2015)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2015)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2015
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.414"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..552
                     /locus_tag="ACHKBL_004947"
     CDS             <1..552
                     /locus_tag="ACHKBL_004947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL_4669 family integrating conjugative element
                     protein"
                     /protein_id="HFO5821668.1"
                     /translation="DIISMPRAIAFAGQATRDSARDNPYADMMLVRLENALTKATETI
                     EKHLAWLDGILKNLPGQISLSDIKSSSPVNIGVYSSSPVGYRCVWLLIGYDRLVMKVF
                     QVFHYGLISRTKRDELLNQGGYAVRRIYGIIQNYKSVSVTRADILSGTARGKEAVNRM
                     GEPDPDVMSGKKRSVYSPPVKAG"
     gene            555..>2015
                     /locus_tag="ACHKBL_004948"
     CDS             555..>2015
                     /locus_tag="ACHKBL_004948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001175889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HFO5821669.1"
                     /translation="MQLFLCEKPSQAKDIARELALSKRGDGYIGDDNIAVTWAIGHLL
                     ELAEPEAYGEQFGSPWRSDVLPLIPEQWQMVVKPKVKKQLIVIRGLLKRASEVVIATD
                     ADREGEVIARELLEYCQYRGRVRRLWLSALDEASIRKALNNILPGEQTALLSEAGKGR
                     ARADWLTGMNLSRLYTLKARERGISGVLSVGRVQTPTLAMVVRRDQEIATFVPVSWWQ
                     VIVWLEKEGLRFRSNWVAAEQYCDDEGRCVNVQAAGAVLGLCRQQSSAVVQDVVQKRE
                     KTPPPLCFSLGTLQEACSRKFGMGAQTVLDIAQSLYETHKATTYPRTDCGYLPASMHE
                     ESSEVLEAVGRSDPSVLPQLAELESGRVSRIWNDKKITAHHAIIPTRQVFDLSLLNGD
                     ELKVYQLIRQHYLAQFLPEQEADVTEATFVIGGQLFRSRGRVNVIIGWKQLFPPENHA
                     DEEKPDSDESGNILPPLIKGELCALDGAEQKELKTVP"
BASE COUNT          487 a          426 c          604 g          498 t
ORIGIN      
        1 gacatcatca gtatgcccag ggctattgcg tttgcgggtc aggcaaccag agattcagcc
       61 agggataatc cttatgcaga tatgatgtta gtcaggctgg aaaatgccct gacgaaggca
      121 actgaaacaa ttgaaaagca tcttgcgtgg cttgacggga tccttaaaaa cctgccggga
      181 cagatctccc tttctgatat taaatcctct tcgccggtaa atattggcgt gtacagtagt
      241 tctccggtcg gttatcgatg tgtatggctt ctgatcggat atgatcgact ggtcatgaag
      301 gtatttcagg tatttcatta cggacttatt tcccgcacaa aacgggatga gttgttgaat
      361 cagggagggt atgcggtacg caggatttac gggattattc aaaattataa atcagtcagt
      421 gttacacgtg ctgatatttt gtccggcaca gcccgtggaa aggaggcggt taaccgcatg
      481 ggggagccgg atcctgacgt gatgagcggt aagaaacgtt ctgtttattc accaccggtt
      541 aaagcggggt gagtatgcaa ctctttctgt gtgaaaaacc ttctcaggcg aaagatatcg
      601 cccgtgaact ggcgctcagt aagcggggcg acggatacat cggagatgac aatattgctg
      661 tcacctgggc gataggacac ctgctggaac tggctgagcc ggaagcttac ggtgaacagt
      721 tcggttcccc atggcgttct gatgtgttgc cgctgatacc ggaacaatgg cagatggtgg
      781 tgaagccgaa ggtgaaaaaa caacttattg tgatccgggg gctgttaaaa cgcgcttctg
      841 aagtggttat tgccacagat gccgatcgtg aaggagaagt gatagccaga gagttgctgg
      901 agtactgtca gtacagaggg cgtgtcaggc gtctgtggtt atctgcactg gatgaggcca
      961 gtatcaggaa ggcgctgaat aatattctcc cgggcgaaca gactgcgctt ctcagtgaag
     1021 ccggtaaggg gcgtgcccgg gctgactggc tgacagggat gaatctgagc cggttgtata
     1081 ccctgaaggc acgggaacgt ggaatttctg gggttttgtc agtgggccgg gtgcagacac
     1141 ccacgctggc aatggtggtc agacgggatc aggagatcgc cacatttgtg ccggtttcct
     1201 ggtggcaggt gattgtctgg ctggaaaagg aggggctccg tttccgcagt aactgggtgg
     1261 cagctgagca gtactgcgat gatgagggac gttgtgtgaa tgtgcaggcg gccggtgctg
     1321 ttctcggtct gtgccgtcag cagtcctctg ctgttgtaca ggatgttgta cagaagaggg
     1381 agaaaacacc gccacctttg tgtttttcgc tgggaacgtt gcaggaagcc tgttcccgga
     1441 agtttggcat gggggctcag accgttctgg atatcgcaca gtccctttat gagactcaca
     1501 aagcaacaac ttatcccagg acagactgtg gttatctgcc tgcctcgatg catgaggaga
     1561 gttctgaagt gcttgaggct gtcgggcgtt cagatccgtc cgtgcttcca caactggctg
     1621 aactggaatc aggacgggta tcccgtatct ggaatgataa aaaaattacg gctcaccatg
     1681 ccattattcc gacccggcag gtttttgacc tcagcttact gaacggtgat gaactgaagg
     1741 tatatcaact gattcgtcag cactatctgg cacagtttct gccggagcag gaagctgatg
     1801 ttactgaggc aacatttgtg attggggggc agttattccg ttcccgcgga agagttaatg
     1861 tgattatcgg gtggaaacag ttatttccac cggaaaatca tgcagatgaa gaaaagcctg
     1921 acagtgatga aagtggaaac atattgcccc ctttgataaa gggagaactc tgtgctctgg
     1981 atggtgctga acagaaggag ctgaaaaccg tacct
//
LOCUS       DAZFLD010000413         2013 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.415, whole genome shotgun sequence.
ACCESSION   DAZFLD010000413 DAZFLD010000000
VERSION     DAZFLD010000413.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2013
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.415"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..714
                     /locus_tag="ACHKBL_004949"
     CDS             <1..714
                     /locus_tag="ACHKBL_004949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000504130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFO5821670.1"
                     /translation="TIRNNLSFLRSAVDEAVTDGLITINPVKLVSASRYHAVNHTANA
                     DDYEVDPFTPAEATAIYNACIYQEWRNLFCFAFNTGLRSSELCALRWSDIDFIGHTVH
                     VQVASVVGVIKGTKTKAGTRKVELNNEAIMALQAQKSFTMMRSDFVFSDPKTRKPWAN
                     SAAIRKKAWIPTLKKAGVRYRNPYQTRHTFATRHISQGANLFWLAGQMGHKGPEMLFR
                     HYGSYLSEYDGKTAISEVI"
     gene            complement(1035..>2013)
                     /gene="ypjA"
                     /locus_tag="ACHKBL_004950"
     CDS             complement(1035..>2013)
                     /gene="ypjA"
                     /locus_tag="ACHKBL_004950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="adhesin-like autotransporter YpjA/EhaD"
                     /protein_id="HFO5821671.1"
                     /translation="PKPDPKPDPTPDPTPTPVPEKRITPSTAAVLNMAATLPLVFDAE
                     LNSIRERLNIMKASPHNNNVWGTTYNTRNNVTTDAGAGFEQTLTGMTVGIDSRNDIPE
                     GIATLGAFMGYSHTHIFFDRGGHGSVGSYSLGGYASWEHESGFYLDGIVKLNRFESNV
                     AGKMSSGGAANGSYRSNGLGGHIETGMRFTDGNWNLTPYASLTGFTADNPEYHLSNGM
                     ESKSVDTRSIYRELGATLSYNMRLGNGMEVEPWLKAAVRKEFVDDNRVKVNNDGNFVN
                     DLSGRRGIYQAGIKASFSSSLSGHLGVGYSHGAGVESPWNAVAGVNWSF"
BASE COUNT          560 a          498 c          445 g          510 t
ORIGIN      
        1 accataagaa acaatctgtc atttttaaga agcgcagtag atgaagcggt cactgacgga
       61 cttataacga taaatcctgt aaaattggtt agtgcaagta gataccatgc agttaaccat
      121 acagccaatg ctgatgatta tgaagtggat cccttcacgc cagcagaggc aacagctatc
      181 tacaacgcct gtatctatca ggaatggaga aatctttttt gtttcgcatt caatacaggc
      241 cttcgaagct cagaattatg tgcattgcgt tggtctgata ttgattttat tgggcatacc
      301 gttcacgtac aggttgcttc tgtcgttggt gtgataaaag gaaccaaaac aaaagctggt
      361 accagaaaag ttgaattaaa caatgaagcc ataatggcat tgcaggccca gaaatcattt
      421 acgatgatga gaagtgattt tgtattcagt gatccaaaaa cacgtaagcc gtgggctaat
      481 tcagctgcaa ttcgtaagaa agcctggatc ccgaccctta aaaaagctgg agttcgttac
      541 cgcaatccat atcagaccag acatacattc gctacacggc atatcagcca gggggcaaat
      601 ttattctggc ttgccggtca aatgggtcac aaaggaccag aaatgctgtt tagacactat
      661 ggttcatatt tatcagaata tgatggcaag actgcgatct cagaagtaat ctagctgctt
      721 aaatatttca aaatatgtac taacgtagga cacagaatag actctgactt acacctgaaa
      781 tacataaaaa catacaaaaa caataagaaa tcttttgttt ttcatatcgt tatgtaattt
      841 gggtgtttgg gttcaactcc cgccagctcc accaatcatg attggacggt gtaaggacaa
      901 caccaacaaa aacaggaagt tagcagtctc agcaagacac cgaccagacg gtgaggagac
      961 ataaaaggat acgcaaagga gccgcggctc ctggtgatat gaaagcccac agatgtgggc
     1021 tttttcgttg atggtcagaa cgaccagttc acaccagcca ccgcgttcca cggggattcc
     1081 acaccggcac catggctata ccccaccccg agatgcccgc ttaacgaact gctgaatgag
     1141 gctttaatac ctgcctggta tattccacgt ctgcccgaca aatcattgac aaaattaccg
     1201 tcattattca ctttcacccg gttatcatcg acaaattctt tgcgcacagc cgccttcagc
     1261 cacggctcaa cttccatgcc gttccccaga cgcatgttgt aactcagcgt tgcgcccagt
     1321 tcacgatata tactacgggt atcgactgat ttcgattcca tgccattgga taaatgatat
     1381 tcggggttat cagcggtgaa ccccgttaac gatgcatacg gcgtcaggtt ccagttacca
     1441 tcggtaaatc gcatcccggt ttcaatgtga ccgcccagcc cgttgctgcg ataactgccg
     1501 ttggcggctc caccgctgct cattttaccg gctacgttac tttcaaagcg gttcagcttc
     1561 acgataccgt ccagatagaa accactttca tgttcccagc tggcatagcc ccccagagaa
     1621 taactgccca cactgccatg ccctccacga tcaaaaaaga tatgtgtatg ggaataaccc
     1681 ataaaagcgc ccagcgtcgc aatcccctca ggaatatcat tacggctatc gatccccacc
     1741 gtcattccgg tcagcgtctg ctcaaacccg gcacccgcat cggtggtgac attattacgg
     1801 gtgttatacg tcgtccccca gacattattg ttgtgtggac tcgctttcat tatgttcaac
     1861 cgctcgcgaa tactgtttag ctcagcatca aagaccaacg gtaatgttgc tgccatattg
     1921 agtacggctg ccgtagaagg cgtgatgcgt ttctccggga cgggtgtcgg cgttggatcg
     1981 ggagtcgggt ctggtttcgg atccggtttt ggg
//
LOCUS       DAZFLD010000414         2008 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.416, whole genome shotgun sequence.
ACCESSION   DAZFLD010000414 DAZFLD010000000
VERSION     DAZFLD010000414.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2008)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2008)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2008
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.416"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..1128)
                     /locus_tag="ACHKBL_004951"
     CDS             complement(<1..1128)
                     /locus_tag="ACHKBL_004951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HFO5821672.1"
                     /translation="MNRTSPYYCRRSVLSLLISALIYAPPGMAAFTPDVIGVVNDETV
                     DGSQKVDERGTTNNTHIINHGQQNVYGGISNGSLIESGGYQDVGGHNNFVGQANNTTI
                     NGGRQSIHDGGISTGTIIESGNQDVYTGGISNGTTIKGGNSHISGGTANGTIIDGGGQ
                     TVTTQGHVDGTTINKSGYQDITQGSLATNTTINGGRQYVEQSTVETTTIKNGGEQRVY
                     ESRALDTTIEGGTQSLDSKSTAKNTQIYSGGTQIVDYTSTSDVIEIYSGGVLDVRGGM
                     ATNVTQHDGAALKVTTYDLTVSGTNSEGAFSIHNNVADNVLLENGGHLDINAYGSANK
                     TIIKDKGTMSVLTNAKADATRIDNGGVMDVAGNATNTIINGG"
     gene            complement(1193..1312)
                     /locus_tag="ACHKBL_004952"
     CDS             complement(1193..1312)
                     /locus_tag="ACHKBL_004952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HFO5821673.1"
                     /translation="MPHSYAEYHHFIMMSLMSGEHNTLALSLQDESPGLVPVG"
     gene            complement(1467..1733)
                     /locus_tag="ACHKBL_004953"
     CDS             complement(1467..1733)
                     /locus_tag="ACHKBL_004953"
                     /inference="COORDINATES: protein motif:HMM:NF012464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFO5821674.1"
                     /translation="MLRVTTSAHNRGSRPGQNKRLRTLSVGDALLVCKLNRLGRSMWH
                     LVVLLEELCERGINFRALAQSIFAQQWGDGSCKSKTICDLRVIV"
BASE COUNT          539 a          500 c          382 g          587 t
ORIGIN      
        1 gccaccatta attatggtat ttgtcgcgtt tccggcaaca tccataacgc cgccattatc
       61 tattcgggtc gcatcagctt tagcattcgt taaaactgac attgttcctt tatctttaat
      121 aatcgtctta tttgccgaac catatgcgtt tatgtctaaa tgaccaccgt tttccagcaa
      181 cacattgtct gccacgttat tgtggatgga gaatgcccct tcactattcg taccgctcac
      241 cgtcaaatcg taagtcgtta ctttcaaagc ggctccatcg tgctgagtaa catttgttgc
      301 cataccacct ctaacatcaa gcacgccacc ggaataaatt tcaataacat ccgaggtgct
      361 agtgtaatca acaatttgcg taccaccaga atagatctga gtattttttg ccgttgactt
      421 actatccaga gactgagttc caccttcaat cgtcgtgtcc agcgcacggc tctcatatac
      481 tctttgctca ccgccatttt taatggtggt tgtttctact gtgctctgtt caacatactg
      541 tcgaccacca tttatggttg tgttcgtcgc cagacttcct tgcgtgatgt cctgataacc
      601 ggacttattt atcgttgtac catcaacatg cccctgagtt gttactgtct gccctccacc
      661 atcaatgatg gttccattcg cagtcccccc acttatgtgt gaattaccac ccttaattgt
      721 cgttccattg ctgataccac ctgtataaac gtcctgattg ccactctcga ttattgtacc
      781 tgtggaaata cccccgtcat gaattgactg tctgcccccg ttaatggttg tattattagc
      841 ctgccccaca aaattgttat gacctcctac atcttgatat ccacccgatt caataagact
      901 tccattagat atcccgccat aaacattctg ctggccatgg ttgataatat gagtgttatt
      961 tgttgtacct cgttcatcta ctttttggct gccatctaca gtctcatcgt ttaccacacc
     1021 aataacatca ggagtgaagg ccgccatccc gggcggggca tatatcaagg cagatatcaa
     1081 taaggaaagt actgagcgac gacaataata gggactggtc ctgttcataa atttcatcct
     1141 ctgaaaagtg aatactgagt agcgtttaag cgaccttagc tttgctgcaa catcagccca
     1201 caggcaccag accaggggat tcatcctgaa gagacagcgc aagtgtattg tgttcaccgc
     1261 tcatcaaaga catcatgatg aaatgatgat attccgcata agaatgaggc attttttaaa
     1321 cgcagtgcgc tgaagtgtgg ttggataaaa aagtcaatct attcaggaaa cacgggcgta
     1381 ttcttttctt tcgacaatca ggcagtcgga aaaataaaat gatttacata atcgtttctg
     1441 atgaatatct tctgctcaca taaaaatcac acaataactc tgagatcgca gattgtttta
     1501 cttttacagc ttccgtcccc ccattgttgg gcaaatatag attgggccag agcacgaaag
     1561 ttaataccac gttcgcacag ctcctccaac agcacgacaa gatgccacat actgcgcccc
     1621 agtcggttca gtttacagac tagcagtgcg tcccctaccg ataatgtcct gagccgtttg
     1681 ttctgtccgg gcctgctgcc acggttatgc gctgaagtgg taactctgag cacgcagttc
     1741 ttcagtcagg cgtggtgcac cgtcacccta ttattgctgg gtaagatcaa aaaactttca
     1801 agcagctaag gaaagttgaa ccagacatta gatgaaatat ttcaaccaat tacagcacca
     1861 gttcagacac cgccaactca tcaaataaat caaggggtta cgtgaaagcg tagccccttt
     1921 tactttggta gtgacagcaa aatggatgca gtgtgaagaa taatcccgtt tactcaatca
     1981 ataatacata ttatttcaat ctacgtta
//
LOCUS       DAZFLD010000415         2005 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.417, whole genome shotgun sequence.
ACCESSION   DAZFLD010000415 DAZFLD010000000
VERSION     DAZFLD010000415.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2005
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.417"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>2005)
                     /locus_tag="ACHKBL_004954"
     CDS             complement(<1..>2005)
                     /locus_tag="ACHKBL_004954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000842442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LPD7 domain-containing protein"
                     /protein_id="HFO5821675.1"
                     /translation="DDRNVLAQETNADATVTTTAHDTGTAETDSPSEPAEIQPVQQAS
                     PEPSAKDKPQENVVSATDNDAENTSSTTTALRSYDTPETSVSDATGTQHTATTQPEEP
                     QQVSMGSADDWIAFAEDLNADAPGNATVHSTDNAETKTLSEPAGMHPVQQATPEPTEE
                     NKPQEEGVMSATDTGEESAAAAIDDDLNTVAEDLNADVTVMTTALHTDTAETASPSEP
                     AEIQPVQQATPEPTAEDKPKEERPVSATETSTENEAARPETPSLFSRLRRFLTGGKSR
                     TAEPPVIQENTLTEAETATEAAAPPSPPETSPEDTSSAEPDTIIFAPRRPDSPQRHNL
                     DEIIKNLTYDELPDNTALCRVDGEPAFLDKLNRLEMVDGASADDKKVLAALAVATNFY
                     GGVIELTGSDAFKQKAMQLIVEYDIKVRMKLPVQRAALEKLRQEKGIAHDAIVTHRPT
                     PELNRLSPEETTMAQPAPPVSPKAETEQTAGSTAETPHPEAPVTAKAEAPRAGTPEQP
                     AASSSAPEKAPVSAQPQQPDTPEEEPPGKLRPGESVTAVLTNYGEKEYAPGKGRCFFV
                     ELTNRSGKREYWGNGLEELVKNHQKGNPVTLTLQARETFRLSGETRDRIRNIWAMTPV
                     SNGITVDNDHPHAGQHILEFPMKTFSQVMSQMQQHWPQIMQEVR"
BASE COUNT          317 a          499 c          613 g          576 t
ORIGIN      
        1 cgaacttcct gcattatctg tggccagtgt tgttgcattt gtgacattac ctgactgaat
       61 gttttcatcg gaaattccag gatatgctga ccagcatgag gatggtcatt atcgacggta
      121 atgccgttac tgacaggagt catggcccag atattacgga tgcgatcccg tgtttcaccg
      181 gacagccgga atgtttctct cgcctgcagc gtcagtgtga cggggttgcc tttctggtgg
      241 tttttgacca gttcttccag gccgtttccc cagtattccc gtttgccact gcggttggtg
      301 agctccacaa aaaagcagcg cccttttccg ggcgcatact ctttttctcc gtagtttgtc
      361 aggacggcag tcacactttc accgggtctg agcttgccgg gcggctcttc ctccggtgtg
      421 tcgggttgct gcggctgtgc actgacaggg gctttttctg gcgcagacga cgaggcggcg
      481 ggttgttccg gtgtgccggc tcggggggct tcagcttttg ccgtgaccgg cgcttccgga
      541 tgcggtgttt cagccgtact gcctgcagtt tgctctgttt ctgctttcgg tgacacaggg
      601 ggggccggtt gtgccattgt tgtctcttcc ggtgacaggc ggttgagttc cggcgtgggt
      661 ctgtgggtga cgatggcatc atgcgctatc cctttctcct ggcggagttt ttccagcgca
      721 gccctctgga ccgggagttt catgcgaacc ttgatgtcat attcgacaat cagctgcatg
      781 gctttttgtt taaaggcatc actgccggtc agctcgataa cgccgccata gaagttggtg
      841 gccacagcaa gggcagccag gacctttttg tcatcagcac tggcaccatc caccatttcc
      901 agacggttga gtttatccag gaatgccggt tcgccgtcga cgcggcagag cgcagtattg
      961 tcgggtaact catcgtatgt aagattttta ataatctcgt ccaggttgtg ccgctgcgga
     1021 ctgtcgggcc ggcggggcgc aaagataatc gtgtccggtt ctgctgacga tgtgtcttcc
     1081 gggctggttt caggtggtga cggtggtgct gcggcctctg tcgcagtttc ggcctccgta
     1141 agcgtgttct cctgaataac gggaggttcg gcggtacgtg attttccacc ggtcagaaaa
     1201 cggcgcaggc ggctgaaaag cgatggtgtt tcagggcggg ctgcttcatt ctctgtgctg
     1261 gtctcagtgg cagacacagg acgctcttcc ttcggtttat cttctgctgt tggctcaggg
     1321 gttgcctgct gtacgggctg aatttctgct ggttcagaag gtgaggctgt ttctgctgta
     1381 tcggtgtgga gagctgtggt catcacagta acatcagcat tgaggtcttc cgcaactgtg
     1441 ttcaggtcgt catcgatagc ggcagcggca ctctcttcac ctgtatcagt ggcagacata
     1501 acgccctctt cctgaggttt attttcttcc gttggttcag gggttgcctg ttgaacaggg
     1561 tgcatcccgg caggttcaga aagtgtcttt gtctccgcgt tgtcggtgct gtgaacagtg
     1621 gcattccccg gagcatcagc attgaggtct tccgcaaatg caatccagtc gtcagcgctg
     1681 cccatggaga cctgctgtgg ttcttcaggt tgcgttgtgg cggtgtgctg tgtcccggtt
     1741 gcgtcggaga cggatgtttc cggtgtgtcg taactccgga gggcagtggt ggtggaagag
     1801 gtgttctctg cgtcattatc agtagcagac actacgttct cctgtggttt atctttagcc
     1861 gatggttcag gactggcctg ctgcactggc tgaatctctg caggttcaga aggtgaatct
     1921 gtttctgctg tgccggtgtc gtgagccgtg gtcgtaaccg tggcgtcagc attggtttct
     1981 tgtgctaata catttcggtc gtcag
//
LOCUS       DAZFLD010000416         1966 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.418, whole genome shotgun sequence.
ACCESSION   DAZFLD010000416 DAZFLD010000000
VERSION     DAZFLD010000416.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1966)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1966)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1966
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.418"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            3..185
                     /locus_tag="ACHKBL_004955"
     CDS             3..185
                     /locus_tag="ACHKBL_004955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001287794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFO5821676.1"
                     /translation="MNQRYRDDHGALVTVTSVEETRVVFMRDGYPHPCMRPMYNFLGK
                     FKPEPRKEPPAGNHTA"
     gene            complement(205..438)
                     /locus_tag="ACHKBL_004956"
     CDS             complement(205..438)
                     /locus_tag="ACHKBL_004956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5821677.1"
                     /translation="MARSDYDIINLSPEHELNEWLAERGYAGQADNRNRVAEIVTRKL
                     QDSFYINVSWDALNTAYSEHPEWFSGLVSGDEN"
     gene            complement(527..1150)
                     /locus_tag="ACHKBL_004957"
     CDS             complement(527..1150)
                     /locus_tag="ACHKBL_004957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFO5821678.1"
                     /translation="MRTRIDYLADKYSFTELNELPRLHQQWQDVLEECRQTEAGPEER
                     LRIALLNVDYVTSFELPFRLLLTRTPQLIAALREEWGISQKNVVFNDKRFGCVYSLKA
                     SLSGVPDTYRYHLSHRIRRVIGNENTSSPYQQIAREVKAPRERLKHALEAGLLVTALD
                     GLFWFGSQRIAADVLRLRKSGMPVVTTTAEVHDNLTGTTRKVPAYHL"
     gene            complement(1239..1748)
                     /locus_tag="ACHKBL_004958"
     CDS             complement(1239..1748)
                     /locus_tag="ACHKBL_004958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5821679.1"
                     /translation="MQITEALISEPGEIRRFVQQAVDHWPNLLAFHFTLYSAEGINGQ
                     QIQTFCSSFHRRVHERITEHNHTVSPSAPVVLRWLREQHEGAQIRCLLLLSQTSICHP
                     RVGVMADEECAQLVDLLQQTWPVISAGGQCRVERCFRVARPGSSGQYVALKTAVQSLM
                     PRGIATIIR"
BASE COUNT          513 a          541 c          440 g          472 t
ORIGIN      
        1 aaatcaatca gcgatacaga gacgatcatg gcgcgcttgt caccgtgacc agtgttgagg
       61 aaacccgggt ggtgtttatg cgggacggct atccgcatcc ctgcatgcgg ccgatgtaca
      121 acttcctggg aaaattcaaa cccgaacccc ggaaagaacc ccctgccggc aatcacactg
      181 cctgatggca gcataatcca cttattaatt ctcatccccg gagacaagcc cagaaaacca
      241 ctccgggtgt tcactgtatg ccgtgttcag cgcatcccaa gaaacgttga tataaaagct
      301 gtcctgcaat ttgcgggtaa caatttctgc cacccggtta cggttatccg cctgtccggc
      361 ataacctctc tctgccagcc actcattcag ttcatgttcc ggagacagat tgataatatc
      421 gtaatcggac cttgccattc ccgtttcctt agcacatgtt ttcaggctcg gggataaaga
      481 atgtcgtact gctgctgcca gagcaatatt ccccctgatg gtcgaatcag agatggtagg
      541 ccggtacttt gcgggttgtt cccgtcagat tatcatgcac ctccgcggtc gttgtcacca
      601 ccggcattcc gctctttctc agcctcagga catcagccgc aatacgctga ctaccgaacc
      661 agaacaggcc gtccagtgca gtcaccagca accccgcctc cagcgcatgc ttcagccgtt
      721 cacggggcgc tttcacttcc cgggcaatct gctggtatgg cgatgatgtg ttttcattcc
      781 caatcacccg gcgaatacga tgagacagat gataccggta tgtatccggt acaccggaaa
      841 ggctggcctt caggctgtac acgcagccaa atcgtttatc attgaacacc acatttttct
      901 ggctgatgcc ccattcttca cgcagcgcgg caatcagttg tggtgtccgg gtaagcaaca
      961 agcgaaaagg cagttcaaaa ctggtgacgt aatccacatt cagcagtgca atacgcagcc
     1021 gttcttccgg cccggcctct gtctgccggc actcctccag aacatcctgc cactgctggt
     1081 gaaggcgtgg aagttcattc agttcagtaa aactgtattt atccgcaaga tagtcaattc
     1141 gtgtacgcat actgaagagt attccgtata aagattcagc tggcaaaact ttatcagtct
     1201 gtaaaaacta acggaagagt cgatatttct cccgtccatc accggatgat tgttgcaata
     1261 ccccgtggca tcagagactg aacagccgtt tttaacgcca cgtattgccc tgatgaacca
     1321 ggccgggcaa cccgaaaaca tctttccacc cggcattgtc cgccagcact tatcaccggc
     1381 caggtctgtt gcagtaaatc caccagttgc gcacattctt catcagccat gacgccgact
     1441 cgcggatggc aaatactcgt ctggctgagc agcaacagac atcgtatctg tgctccttca
     1501 tgttgttccc gcaaccagcg taataccacc ggcgctgatg ggctgaccgt gtgattatgc
     1561 tccgtaatac gttcgtgtac ccgtcggtga aaggaactac aaaatgtctg aatctgttga
     1621 ccattgattc cttctgccga atagagcgtg aagtgaaatg ccaacagatt tggccagtgg
     1681 tccaccgcct gctgaacaaa acgccggatt tcccccggct ctgaaattaa ggcttcggtt
     1741 atttgcacga aatgttcctt ttcaacccct gttgaatttt gtcaagccgg tgcaggcaca
     1801 gtaaagcgcg catcaacacc tgaaaggtac gatacaaaca caagttacaa acaataagaa
     1861 cggaaggaca attaatcagt gaggaataat aactaaatta accaaaagaa tgttactgac
     1921 aatactacca atacttaaat agcaatatat taattaatgt aagaac
//
LOCUS       DAZFLD010000417         1964 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.419, whole genome shotgun sequence.
ACCESSION   DAZFLD010000417 DAZFLD010000000
VERSION     DAZFLD010000417.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1964
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.419"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..160)
                     /locus_tag="ACHKBL_004959"
     CDS             complement(<1..160)
                     /locus_tag="ACHKBL_004959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03756 family integrating conjugative element
                     protein"
                     /protein_id="HFO5821680.1"
                     /translation="MISCTSRHRAGVLLLSMAVLPVAEASLNTAQIVASAASQSCISW
                     RVSGICYWL"
     gene            complement(157..552)
                     /locus_tag="ACHKBL_004960"
     CDS             complement(157..552)
                     /locus_tag="ACHKBL_004960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000286193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03757 family integrating conjugative element
                     protein"
                     /protein_id="HFO5821681.1"
                     /translation="MYRLLLYSFLILPVAASAGTTIYTDSHQRPMNPPAGVRVVLLDA
                     PEQTQDTFFGVLPAEPAEASARVMERMQSPEWQSFQAGLAEHYRALAHAWSLGLKKYP
                     AVVFDDSEVVYGTTDVVLAEQLRTGGGQP"
     gene            complement(688..1320)
                     /locus_tag="ACHKBL_004961"
     CDS             complement(688..1320)
                     /locus_tag="ACHKBL_004961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000924347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFO5821682.1"
                     /translation="MLLIAGHLRPAFQAHTGRFLIADLSEEVVRRYLRFCTPLSGMAV
                     SPSSLLLQIRPQMSVLAECLIRQLITDEPSSVYFRDAVAFSSLALFEEHPDFHALLTG
                     CIRSCTVRVKNIISADLSRPWMLGEVASLLYISESLLKKKLQEELTTFSELLLKERMQ
                     YAAQLLSAGENRISRVAELCGYASASYFISVFRRYYGITPNQWLPVMQPD"
     gene            complement(1512..1850)
                     /locus_tag="ACHKBL_004962"
     CDS             complement(1512..1850)
                     /locus_tag="ACHKBL_004962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5821683.1"
                     /translation="MTISRGRNISCSALTLNNERLYAEKTILALCISAPFLVAGCDDA
                     TKENVSKKAADIKEAAQTQLSDVKDAAEEVKMQAAGKVAEVSEAAQSGLSEVKDTAPE
                     KGAEISVTEE"
BASE COUNT          531 a          547 c          441 g          445 t
ORIGIN      
        1 acagccagta gcaaatcccg ctgacccgcc agctgataca gctctgtgag gctgcactgg
       61 ccactatctg cgcggtgttc agcgatgcct ctgccaccgg caataccgcc atacttaaca
      121 gcagaacacc ggcgcgatga cgtgatgtgc aggaaatcat ggctgccctc cccctgtacg
      181 cagttgttct gccagcacca cgtctgtggt tccgtagacc acttcactgt catcaaaaac
      241 cacagccgga tattttttca gtccaaggga ccaggcatgt gctaatgcac ggtagtgttc
      301 agcaagccct gcctgaaacg actgccattc aggcgactgc atccgttcca tgacacgggc
      361 agatgcttct gcaggctctg cgggaagtac cccgaaaaat gtatcctgcg tctgctcagg
      421 agcatcaagc aggacaacac gaacacctgc tggcgggttc atggggcgct ggtgcgaatc
      481 ggtataaatt gttgtaccgg cagatgcagc caccggtagt atcaggaagg aatacagtaa
      541 cagtcggtac atgatgcgac tccgtcagac aactggtatc tgacagcgtg cgcaaaggca
      601 aagggacaat cagcaataaa ttcaaaaccg gatgataaaa attaagcctc ttcctctgaa
      661 taacgcacac tctgcatctg aatcaaatta atccggttgc atcacaggaa gccactgatt
      721 cggggtaata ccataataac gcctgaacac agaaataaaa taagaagcac tggcatagcc
      781 acacaactct gccacccggg aaatacggtt ttctcccgca gacaaaagct gagcggcata
      841 ctgcattctc tctttcagaa gaagctcact gaaggtcgtc agctcttcct gaagcttttt
      901 cttcagtaaa ctctcactta tatataacag actggccact tcacccagca tccatggacg
      961 ggacaaatca gcactgataa tattcttcac ccggacagtg cagctccgga tacaccccgt
     1021 gagcagggcg tggaaatccg gatgttcctc aaacagcgcc agactggaaa aagccaccgc
     1081 atccctgaag tacaccgacg atggttcatc tgtaataagt tgtctgatca gacattccgc
     1141 cagcacggac atctgtggcc ggatctgtaa aagcaaagac gacgggctga ctgccatacc
     1201 ggataatggc gtgcagaacc gcagataccg cctcaccacc tcctcactga ggtcagcgat
     1261 aaggaaacgt ccggtatgag cctgaaaagc agggcgcaga tgcccggcaa tcagaagcat
     1321 tgtgccggca ggtatgacag agttcctgtg ctccagacaa agcattgtct gtttgagagc
     1381 cataatgaca acgcaggtac agggcatcgg atatccggta aatatgttga tttattaaag
     1441 agacagacat gccccgtata taaaattccg tgtcaccggg tgctgaaaaa aactgcaccg
     1501 acggcgctct tttactcctc cgttacggat atttcagccc ccttttcagg agcggtatcc
     1561 ttcacctcgc tgagcccgga ctgagccgct tcagaaacct ctgccacctt tccggcagcc
     1621 tgcattttca cttcctccgc ggcatccttc acatcactga gttgagtctg cgcagcttcc
     1681 tttatatctg ccgccttctt acttacattc tcctttgtcg catcatcaca gcccgcaacc
     1741 agaaatggtg cggaaataca taacgccaga attgtttttt cagcatacaa tctctcatta
     1801 ttaagtgtta acgcagaaca gcttatattt cgtccccggg aaatggtcaa aagacaacca
     1861 gaacatcaca gtgtcagggc acactcccgg tatatgcttt tataaccata acatgctgtt
     1921 cctgatatta ccggaaatgt gcccactaca tttaacctgt tttc
//
LOCUS       DAZFLD010000418         1960 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.420, whole genome shotgun sequence.
ACCESSION   DAZFLD010000418 DAZFLD010000000
VERSION     DAZFLD010000418.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1960)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1960)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1960
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.420"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..516)
                     /locus_tag="ACHKBL_004963"
     CDS             complement(<1..516)
                     /locus_tag="ACHKBL_004963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease"
                     /protein_id="HFO5821684.1"
                     /translation="MILQTHTIAETSNFIVLDKYIKAEQTGDSYQSESDLERELIQDL
                     QNQGYEFISVKSQSAMLANVREQLQSLNGVVFNDSEWRRFTEQYLDNPGDGILDKTRK
                     IHIDYICDFIFDNGRLENIYLIDKKNLMRNKVQIIRQFEQAGSHSNRYDVTILVNGLP
                     LVQIELKKRGVA"
     gene            complement(636..1851)
                     /locus_tag="ACHKBL_004964"
                     /pseudo
     CDS             complement(636..1851)
                     /locus_tag="ACHKBL_004964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001293189.1,RefSeq:WP_001538201.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
     gene            complement(1848..>1960)
                     /locus_tag="ACHKBL_004965"
     CDS             complement(1848..>1960)
                     /locus_tag="ACHKBL_004965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type I restriction endonuclease subunit M"
                     /protein_id="HFO5821685.1"
                     /translation="IAELNAELKTTVSKIDQLRKDIDAIVAEIEGCGVQK"
BASE COUNT          513 a          443 c          404 g          600 t
ORIGIN      
        1 cgccacgcca cgctttttca gttcgatttg caccagcggc aaaccattaa ccaggatagt
       61 gacgtcataa cggttactgt gagaacctgc ctgttcaaac tgccggataa tctgcacttt
      121 gttgcgcatg agattctttt tatctatcag atagatattc tcaagacgcc cgttgtcaaa
      181 aataaagtca cagatatagt cgatatggat tttacgggtc ttatccagaa tgccatcgcc
      241 agggttgtcc agatactgct ccgtgaaacg ccgccattcg ctgtcattaa acaccacgcc
      301 attgaggctc tgaagctgtt cccggacatt agccagcatc gccgactgtg attttacgga
      361 aataaattca tagccctgat tctgcaggtc ctgaatcagt tcacgttcca ggtccgattc
      421 actttggtag ctgtcgcctg tttgctcagc cttgatgtac ttatcaagaa cgataaagtt
      481 actggtttca gcaatggtgt gtgtctgaag aatcatagcg catcctttgt gccgtctggc
      541 ggggccggaa gggagttaag ggtgacttcc ggcgtgtaaa aatggtttat atactgaccg
      601 gatgttaagg tggcccggtc ggtagcaatt gtcaattagt tactgacggt ttcaggtttc
      661 gggaaactga acagtaaatc acgatagtac tcgtattgtt tctggcgcaa ctcgatttca
      721 cgcgggagac cttcggtgat ggagttggtc agggtgtcga atttatcgag tatttcgaca
      781 atgcgagctt gttcttctga aggtggtaca ggtattggga attttaaaaa cattggtcga
      841 cgaagtgagg taaccgatga acttactgat gctttttgaa tataggtaaa aaaatatgct
      901 gttatatagt gatatacaaa tcgcggattc ataaatgcat ttgggacaat acgatatgct
      961 ctctgatgca aagcatattt acctttagcg taatgaaata ccttccccac cccggcacca
     1021 tctccagccg taatgattgc tgtttcatca aaatcaaata catttaactt taatggttca
     1081 cgtccacgtg cataaaaaat atatttgcca tgttctatag catcttgcgt atcatggtta
     1141 cctgttccta taacagccac ttcccccaaa gtcttccact caatccgaat accatccatg
     1201 gcgtccggaa ggatgtaatt acccctcccg caatatcttt gaaattcaac aacttatctc
     1261 gatagtagtt gtattgtttt ttacgcatgt taagctctgc tgtcagctct gctgtcagct
     1321 ctgctgtcag ctctgctgtc agggcgctga atttgtccag aattcgcacg atttcagact
     1381 ggatggcaag ggatttttcc ggattatccg ggcaggggat tggtacaggt atattttcaa
     1441 cctgatgact cattaacttg ggattcccat tcctgagtaa acatgttttt tggcctctat
     1501 tgttagccag taaaacagaa atttgtagat taatggatat ttacttttta atgtaattag
     1561 gccgcaaaca ttagttattg aaaatttacc tgttcgatag aacacagtgc ctgcatttgc
     1621 gccatctgtt gtccagctta tatattcgcc atcaaaatcg aaggtattga tagaacctat
     1681 tttcccatta tttgcagttt gagagctata gacaggatat gggccaaaat tttcagttaa
     1741 atatcccttt gacataactc tgcctcttct taaggctgag acttccccca atggcaacca
     1801 ctcaacctca accccatcca gcaatttttc cagataactc aactcgctca tttctgcacc
     1861 ccgcagcctt caatttcagc cacaatcgca tcaatatctt tacgcaactg gtcgattttg
     1921 ctgaccgtgg ttttcagctc agcattcagc tcagcaatat
//
LOCUS       DAZFLD010000419         1948 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.421, whole genome shotgun sequence.
ACCESSION   DAZFLD010000419 DAZFLD010000000
VERSION     DAZFLD010000419.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1948
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.421"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..211
                     /locus_tag="ACHKBL_004966"
     CDS             <1..211
                     /locus_tag="ACHKBL_004966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ZinT/AdcA family metal-binding protein"
                     /protein_id="HFO5821686.1"
                     /translation="LFECKDPESKAPKYIQFSDHIIAPRKSSHFHIFMGNDSQQSLLN
                     EMENWPTYYPYQLSSEEVVEEMMSH"
     gene            complement(881..970)
                     /locus_tag="ACHKBL_004967"
     tRNA            complement(881..970)
                     /locus_tag="ACHKBL_004967"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(934..936),aa:Ser,seq:cga)
     gene            1064..1861
                     /gene="mtfA"
                     /locus_tag="ACHKBL_004968"
     CDS             1064..1861
                     /gene="mtfA"
                     /locus_tag="ACHKBL_004968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DgsA anti-repressor MtfA"
                     /protein_id="HFO5821687.1"
                     /translation="MIKWPWKVQESAHQTALPWQEALSIPLLTCLTEQEQSKLVTLAE
                     RFLQQKRLVPLQGFELDSLRSCRIALLFCLPVLELGLEWLDGFHEVLIYPAPFVVDDE
                     WEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEVAHKLDTRNGD
                     RASGVPFISLREVAGWEHDLHAAMNNIQEEIELVGENAASIDAYAASDPAECFAVLSE
                     YFFSAPELFAPRFPSLWQRFCQFYQQDPLQRLHHANDTDSFSATNVH"
BASE COUNT          550 a          405 c          424 g          569 t
ORIGIN      
        1 cttattcgaa tgtaaagatc ctgaaagcaa agcccctaaa tatatacaat ttagcgatca
       61 tataattgca ccacgaaaat catctcattt tcacattttt atgggtaatg attcccagca
      121 atctttattg aatgaaatgg aaaactggcc aacgtattat ccctatcagt tgagtagcga
      181 agaagtggtc gaggagatga tgtctcattg atttttttgc gctgacttca gttatttaac
      241 agaacagtgc ctggtagtaa caacaataat ttaccaagac aaataagtaa gacaaactca
      301 gacacgctca tacttgagtc gtgtcttttt tattatgtct gtagaaaaaa actgcgaaaa
      361 aggcgaacaa taaatacaca actcagccat acagactggc agcgcaaatg tttccaggag
      421 caagtcatct gtcatggtaa agctaaccag aaatataggt taattttggt gtttaaacct
      481 tttttacttg ttagtatgat acaaaggctt tcaaaaaagc tgggaaattc aacaactctt
      541 ggaaattatg gactttgtcc cgcggcttta atctgacggc cgcgttcctt ttttggctca
      601 attattgacc ccttctctac gcaacttcag ttcccgccag caactttgcg gcagtttcat
      661 aggatcaatg tctaaaagaa taatggtgat cgataagaaa acgactgaat aactgtagat
      721 tttcgctcga aaccttcctg tcagctccat agcgaatcaa gtgctgaatg tcacagtatc
      781 gaacagaaaa cagtgacgat ctaacccttc aagaatattc tacgattgtt ctgtttagga
      841 aaagcaaggc gggaagtcgg gagataagtc attgataaag tggcggagag agggggattt
      901 gaacccccgg tagagttgcc cctactccgg ttttcgagac cggtccgttc agccgctccg
      961 gcatctctcc gttcagatgg ttgccatgat gccaggaaat ttggcatttt aacagtccct
     1021 gtccgtgcaa ttttgttcaa gtgacgagtt tgcgagcaaa acgatgatta agtggccctg
     1081 gaaagtacaa gaatcagcac atcaaactgc ccttccctgg caggaagcac tatcgatccc
     1141 ccttttaacg tgtctgacgg aacaggaaca aagcaaatta gtcactcttg ccgaacgttt
     1201 tttacagcaa aagcggcttg ttcctttaca gggctttgaa ctggattcat taagaagctg
     1261 ccggatagca cttctatttt gcctgcccgt tctggagtta ggactggaat ggctggatgg
     1321 ttttcatgaa gtcttaattt atcctgcgcc atttgtggtc gatgatgaat gggaagacga
     1381 tatcggtctg gtgcataacc aacgtattgt tcagtcaggt cagagctggc agcaagggcc
     1441 tatcgttttg aactggttgg atatacaaga ttcttttgat gcttctggtt ttaacctgat
     1501 tattcatgaa gtcgctcata agctggacac ccgtaacggc gatcgcgcca gcggagttcc
     1561 ctttatttcg ttgcgtgagg ttgctggctg ggaacacgat cttcatgctg caatgaacaa
     1621 cattcaggaa gaaatcgaat tagttggtga gaatgcggcg agcattgatg cttatgctgc
     1681 cagtgatcct gctgaatgtt ttgccgtact ttctgaatat ttctttagcg ccccagaact
     1741 ttttgctcct cgtttccctt cattgtggca acgtttctgt caattttatc aacaagatcc
     1801 tttgcagaga ctgcatcacg ctaatgatac agactcgttt tcggcgacga atgttcatta
     1861 attaacaact ttgcagatta attaaccaat tgaaatggct tatgaaattt agtgttgaca
     1921 gacaaggtac cgctaagtaa tatgcgcc
//
LOCUS       DAZFLD010000420         1940 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.422, whole genome shotgun sequence.
ACCESSION   DAZFLD010000420 DAZFLD010000000
VERSION     DAZFLD010000420.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1940
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.422"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(86..847)
                     /locus_tag="ACHKBL_004969"
     CDS             complement(86..847)
                     /locus_tag="ACHKBL_004969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HFO5821688.1"
                     /translation="MKKIIAVSALAVVGMFSGLALADRGKTGFYMTGKAGASVVTQTD
                     QRFRQDFGDDVYKYKGGDKNDTVFGAGLAVGYDFYQHYNVPVRTEVEFYGRGAADSRY
                     TLDTWYSPMVGGGREDTQNRLSVNTLMVNTYYDFRNSSAFTSSAFTPWVSVGLGYARI
                     HHKATYTDTSWNKSGEVSNFSALHYPGYDNNFAWSFGAGVRYDVTPDIALDLSYRYLD
                     AGDASVSYKDEWGDKYKSEVDVKSHDIMLGVTYNF"
     gene            1637..>1940
                     /gene="subA"
                     /locus_tag="ACHKBL_004970"
     CDS             1637..>1940
                     /gene="subA"
                     /locus_tag="ACHKBL_004970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001380856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="subtilase AB5 cytotoxin subunit A"
                     /protein_id="HFO5821689.1"
                     /translation="MLKNLRLHILFLLFISVSVRAEKPWYFDAIGLTETTMSLTDKNT
                     PVVVSVVDSGVAFVGGLSDSEFAKFSFTQDGSPFPVKEPEALYIHGTAMASLIASRH"
BASE COUNT          542 a          466 c          407 g          525 t
ORIGIN      
        1 agacaaccct gcagtataat tacggcaggg agtttccgtt taattacaga agacaggtaa
       61 ttattatctt tcaggagtag tttcgtcaga agttataagt cacaccaagc atgatgtcat
      121 gacttttaac atcaacttct gatttatatt tatcgcccca ctcatccttg taactcacac
      181 tggcatcacc tgcatcaaga tagcgatagc tgaggtccag agcgatatcc ggggttacgt
      241 catagcggac acctgcccca aaactccatg caaaattgtt atcatatccc gggtaatgca
      301 acgcagaaaa attgctcacc tcaccagatt tattccagga ggtatcagta tatgttgcct
      361 tatgatgtat ccgtgcataa cccagtccaa cagacaccca tggagtgaat gcactgctgg
      421 tgaatgcact gctgtttctg aagtcataat acgtattcac catcagagta ttcacactga
      481 gcctgttctg tgtgtcttcc cgaccaccac cgaccatcgg agagtaccat gtatccagtg
      541 tataacggga gtccgcagct ccgcggccat agaattccac ttccgtgcgc acaggaacat
      601 tgtaatgctg ataaaaatca tagcccaccg caaggccggc accgaatacc gtatcgttct
      661 tatcgccgcc cttatactta taaacatcgt ccccaaagtc ctgacgaaag cgctggtcag
      721 tctgagtcac aaccgaagca ccagctttac cggtcatata aaagcctgtt ttgcctctat
      781 cagccagggc caggcctgaa aacatgccca ccacagcaag cgctgaaacc gcaataatct
      841 ttttcatttc agttatctcc gctatcactt tccccgaacg ataaacgtgg ttgcttattc
      901 taaacccatg caaaagacag ggtaaaaaga gaaaaatcga tcgaatcgat cgacaaaatc
      961 gatcatgcat gatgaagatt tcttatcgaa tacataaaaa tagtgatggc aatcgtggca
     1021 ggagcttacg tcaggccctg aagtactgac acaggcattg atggcaggtg atataaggag
     1081 tgctccaggg aatgactccc gactgaccca ggcactgtcc cgttgtcaac ccgtatggtt
     1141 actgctctga ctcatttcct gtcgtcagac gttcaactct ggaccactgg ttatgacgac
     1201 aggtgtcgct catttgtcag cactataacg gtcaaagatc gtggcattga cacattctcc
     1261 agaggtaaat ttctcaagag gtaacaactc tgccaggagg atatctgcag agagttacat
     1321 tcgtttgggt tcatatctgt gccgcgcgtt ttccttccat cagtgaacaa acattcagtc
     1381 ccatgacgac cgggcaaata gcgtgttcac agatattgct gtccaggtac gaagtaccat
     1441 aatggatatt ggtaagttat tgggtgaccc ccctccatga aaaaaccaaa tataaaaatg
     1501 aatgtttcgg gagagacttc actcgcttta ttgatagtga ttgtttatct tttactgaag
     1561 acaggatatt tcagatgtga tatccctgta acatattgac cagcaatact ttttatttgt
     1621 accactggag gatattgtgc tgaaaaattt acggctgcat attctgttcc tgctttttat
     1681 ctcagtgtca gtcagggcgg aaaaaccatg gtattttgat gcaattggtc tgacagaaac
     1741 aaccatgtcg ctcacagaca aaaatacccc ggttgtcgta tctgttgttg attccggagt
     1801 ggcatttgta ggaggcctca gcgacagcga atttgccaaa ttcagtttta ctcaggatgg
     1861 ttcgccattc ccggtaaaag agcctgaagc tttatatatt catggtactg ccatggcttc
     1921 actcattgcc tcacgtcatg
//
LOCUS       DAZFLD010000421         1932 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.423, whole genome shotgun sequence.
ACCESSION   DAZFLD010000421 DAZFLD010000000
VERSION     DAZFLD010000421.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1932
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.423"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            61..1230
                     /locus_tag="ACHKBL_004971"
     CDS             61..1230
                     /locus_tag="ACHKBL_004971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001532118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFO5821690.1"
                     /translation="MFRKIKIRKMTLNRALDKYLKTVSVHKKGHLQEFYRVNVIKRHP
                     MADRYMDEITTVDIATYRDQRLAQINPRTGSQITGNTVRLELALLSSLFNIARVEWGT
                     CRMNPVELVRKPKISSGRDRRLTSGEERRLSRYFRDKNQQLYVIFHLALETAMRQGEI
                     LTLRWEHLDLQHGVAHLPETKNGLPRDVPLSRKARNYLQILPQQINGNVFSYTSSGFK
                     SAWRTALLDLKIENLHFHDLRHEAISRFFELGTLNVIEVAAISGHRSLNMLKRYTHLR
                     AYQLVSKLDARRKQTSKISPYFVPYPATVGCRNGLFVVTLHDFDLETHAETRELAISH
                     ASVLLLRTLAQAAQRGERVPTPGELPANIDERVMICPLTNDQLMTKATTILTSPC"
     gene            1271..1552
                     /locus_tag="ACHKBL_004972"
     CDS             1271..1552
                     /locus_tag="ACHKBL_004972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001532117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5821691.1"
                     /translation="MKHVSTRAANADNNRIVTGRNINEIAFTPPIHANSVLGWTGTVI
                     LTGNADEVKAMLSAAATGHSGCMVTPSPVLAHDTWQRIRLLMGKMMDAK"
     gene            1745..>1932
                     /locus_tag="ACHKBL_004973"
     CDS             1745..>1932
                     /locus_tag="ACHKBL_004973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001532115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5821692.1"
                     /translation="MVILSHFLIQIYMWPDFLSEKNIPRLIAAMVALYLSAVTVIPAF
                     LLSDDSERFKNLSPVKQT"
BASE COUNT          533 a          501 c          425 g          473 t
ORIGIN      
        1 cgatattgca tttctgattc agtattccta ccttcctcgg atttatcgtg gaaggcagat
       61 atgtttcgaa aaataaaaat tcgcaaaatg actctcaacc gggcgctgga caaatatctg
      121 aaaactgttt ccgtccataa aaaagggcat ctgcaggagt tttatcgcgt caatgtgatt
      181 aaacgtcacc ctatggccga tcgctatatg gatgaaatca cgaccgttga tatcgccact
      241 taccgggatc aacgtctggc tcagattaac ccacgaacag ggagtcaaat caccggaaac
      301 accgtccgtc ttgaacttgc tcttttatca tcattattca atatcgcccg tgtggaatgg
      361 gggacatgcc gtatgaatcc tgttgaactg gtccgaaaac ccaaaatcag tagtgggcga
      421 gatcgtcgcc tgacttctgg cgaagaacgc cgactctccc gctatttcag agataaaaat
      481 cagcagctct acgttatctt ccatctggcc ctggaaacag caatgcgaca gggggaaatt
      541 ctcacattac gctgggaaca cctcgatttg cagcacggcg tggcgcatct tcctgaaacg
      601 aaaaatggtt taccacgcga tgtcccgctc tcccggaaag ctcgcaacta cctgcaaatt
      661 ctgccacaac agataaacgg caatgtgttc agttacacct catccggttt caaaagcgcc
      721 tggcgtaccg cacttctgga cctgaaaata gaaaatctgc attttcacga tctccgtcat
      781 gaagccatca gccgattttt cgaacttggt acattgaatg tcattgaagt tgccgctatt
      841 tcaggccacc gctccctgaa catgttaaaa cggtacacgc atctccgggc atatcaactg
      901 gtcagcaaac tcgatgcaag gcgtaaacag accagcaaaa tttcccctta ttttgttccc
      961 taccctgcaa ctgtcgggtg cagaaacggg ctgtttgtcg tgacactgca cgatttcgac
     1021 cttgaaaccc atgcagaaac ccgggaactg gcaatatctc atgccagcgt attgttgctg
     1081 cgcacactgg cacaggcggc tcagcggggt gaacgcgttc ccacaccagg tgagctaccc
     1141 gcaaacattg atgaacgggt catgatttgt ccgttaacaa acgaccagct tatgacaaaa
     1201 gcgaccacta ttctcacatc cccttgctga gcaaacaatt ttgttcaaaa ttaacgcaat
     1261 gaggttcttt atgaaacacg tatccacccg tgctgccaat gcggataata atcgcatcgt
     1321 aactggccgg aacattaacg aaatcgcatt cacaccacca attcacgcca actccgtgtt
     1381 ggggtggacg gggacggtta tccttactgg caatgccgac gaagttaaag ccatgctttc
     1441 tgcagccgct accgggcaca gtggttgcat ggtaacacca tcacctgtac tggctcatga
     1501 cacctggcag cgtatccgcc tgctgatggg caagatgatg gatgcaaaat aaacattcat
     1561 tgctccggaa cataaaaata aagccgggaa cgccgggcac tggcgtgctg ctcagcggtg
     1621 taaacacgtt cccacgtcag gcgagctgcc cgcaaacatt gatgaacggg gcatgatttg
     1681 tccggtaaca aactgaaggc atatcagaat gaaaataaac aattgcttcc ggggtaaatt
     1741 aatcatggtc attctgtctc acttcctgat acagatatat atgtggccag attttctgtc
     1801 agagaaaaac attccccgtc tcattgccgc tatggtagct ctgtacctgt ctgcagtaac
     1861 tgtcattccg gcatttttac tgtcagatga cagtgagaga tttaaaaatc tctctcctgt
     1921 aaagcaaaca at
//
LOCUS       DAZFLD010000422         1922 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.424, whole genome shotgun sequence.
ACCESSION   DAZFLD010000422 DAZFLD010000000
VERSION     DAZFLD010000422.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1922
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.424"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..342)
                     /locus_tag="ACHKBL_004974"
     CDS             complement(<1..342)
                     /locus_tag="ACHKBL_004974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000382418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFO5821693.1"
                     /translation="MDIFNTPISRKGTYCTQWDFCEDRFGVKDVLPFSISDMDLPIPD
                     AITRALKKRLEHPILGYSRWQHGEYLNAIVNWYHQQYQTDIKPEWITYSPSVMYSIAK
                     AIELLTSYGDNI"
     gene            complement(342..1922)
                     /locus_tag="ACHKBL_004975"
     CDS             complement(342..1922)
                     /locus_tag="ACHKBL_004975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000810566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFO5821694.1"
                     /translation="MKQKKAWSFFQSLGKAFMYPIALLSVCGMMLGLGSGLASDDMAK
                     LIPFLAIPIIKTILDFIVSLGLFAFVNLPVLFAIAIPLGLLKDKEDKAYGAFSGLIGF
                     MAMHLGTNFYLKQHDLLVVADQMSTHGQNIILGIQSYNTSVLGGIVAGLLVASMYKKI
                     VNLRIPESLGFYSGPRLVPIITLIVMSGFGLIIPFIWPPFFNLFMLIGHWISTSGPVG
                     YFFYAVAERVTIPFGLNHLVTSVFRFTPIGGSAVIGGEEYYGTLNMFMAYVKENAVIP
                     LDLAGKMEQGKLMIQYGLAGAALAMYRTAHAQNRKAIKALLISGVLTVIIGGVSEPIE
                     FLFLFVSPLLFVFHAFMNGFANMVLPYMGVKMGFTGDLIQFISFGVLRGTRTGWPIAV
                     CVEVAYFFIYYFVFRWTILKFNLMTVGREESSPVTLNAHEDTAIADTPTLDKSELQAA
                     EQMVKALGGKENIKSLDNCVTRLRLTIADMGLIDEVAIKRAGGIAVVKLDQNTLQVII
                     GTKVIALRRDMDNYMGMR"
BASE COUNT          598 a          458 c          369 g          497 t
ORIGIN      
        1 aatattatca ccatacgatg tcaataactc tattgccttt gcaatagagt acatcacact
       61 ggggctatag gttatccatt caggtttaat gtctgtttga tattgttgat gataccagtt
      121 tactatcgca ttcagatatt caccatgttg ccaacgactg taccctaaaa tcgggtgttc
      181 caggcgtttt tttaatgctc tggtgatagc gtctggtatc ggaagatcca tatctgaaat
      241 agagaagggt aatacatctt ttacaccaaa tctgtcctcg caaaagtccc attgggtgca
      301 atatgttcct ttacgggaaa taggagtatt aaaaatatcc atcagcgcat ccccatgtag
      361 ttatccatat ccctacgcag ggcgatgact ttagtgccga taatgacttg tagggtattt
      421 tgatcaagtt taacaaccgc aatcccgcca gctcttttta ttgcaacttc atcaatcaat
      481 cccatatctg cgattgttaa acgtaaacga gttacgcaat tatccagtga cttaatattc
      541 tctttaccac caagtgcctt aaccatctgc tccgctgctt gcaactctga tttatcaaga
      601 gttggggtat ccgctatagc cgtatcttcg tgagcgttca gcgtgacagg actggactct
      661 tcacgtccta cggtcatcag gttaaatttg agaatggtcc aacggaacac aaagtaataa
      721 atgaagaaat aggccacttc aacacacacc gcgatcggcc aacctgttct tgtgccacgc
      781 aatacgccaa agctaataaa ttgaatcaga tcgccagtaa atcccatctt cacgcccata
      841 tatggcaaga ccatgttggc gaatccattc ataaaggcat ggaagacaaa cagcagcgga
      901 ctgacaaata agaacagaaa ttcaatcggt tcgctgacgc caccaataat aaccgtaagc
      961 acaccggaaa taagcaatgc tttgatagcc tttctgtttt gagcatgagc agtgcgatac
     1021 atggccagtg cagcaccagc cagaccgtac tgaatcatca gtttgccctg ctccattttc
     1081 cccgccaaat ccagcggaat gaccgcattc tctttgacgt acgccataaa catgttcagg
     1141 gtgccgtaat actcttcgcc gccaatcaca gccgaaccgc cgattggcgt aaagcggaac
     1201 actgacgtca ccagatggtt taagccaaaa ggaatcgtca cacgttcggc aactgcatag
     1261 aagaaataac caacaggacc ggaagttgat atccagtggc caatgagcat gaagagattg
     1321 aaaaatggcg gccagataaa aggaatgatc agaccaaatc cactcatcac aatcagtgta
     1381 atgataggca ccagacgtgg gccgctataa aaacctaatg attctggaat gcgtaaatta
     1441 acgatctttt tatacatgct ggcgactaat aacccagcaa caattccccc taacacgctg
     1501 gtattgtagg actggatccc cagaatgatg ttttgcccat gtgtcgacat ttggtcagca
     1561 acgaccaata agtcgtgctg tttaagataa aagttcgttc ccagatgcat cgccataaaa
     1621 ccaattaagc cagaaaaagc accataggct ttatcctctt tatcttttaa taatcctaag
     1681 ggaatcgcta tcgcaaacaa tacaggtaaa ttaacaaagg caaacaaacc aagactaaca
     1741 atgaaatcaa gtatggtttt aattattgga atagccagaa atggaattaa ctttgccata
     1801 tcatcactgg ctaaaccact tcccagccct agcatcatgc cacatacact tagcagagca
     1861 atgggataca taaatgcctt ccccaggctc tgaaaaaaac tccaggcttt cttttgtttc
     1921 at
//
LOCUS       DAZFLD010000423         1921 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.425, whole genome shotgun sequence.
ACCESSION   DAZFLD010000423 DAZFLD010000000
VERSION     DAZFLD010000423.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1921
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.425"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..982
                     /locus_tag="ACHKBL_004976"
     CDS             <1..982
                     /locus_tag="ACHKBL_004976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5821695.1"
                     /translation="DDTRTSHALVKHNYQRLLDKIDSGSDRYSPMEYWRALHNPDYRD
                     VLQKTIVKHPSDWYFKKDDAIWQPFLNALKKDAPEWKKYSEDFLDKMAWMQDVTTEKL
                     GPLLWHMHPIMFLGCMKKSSGIWPLGKTSERYESGGRGPGVISSGKGDHGGVSYGMYQ
                     LSSKMGTVNKYIIMSKYKDLFEGKKPATPEFNEIWKKIALIHKEEFAQDQHLFIKKTH
                     YQVQLDYLASKGLNFSHSLAAINDMIWSTSVQYGPTTGLISRALKGKNLETLTDADVI
                     TIVQDYKINHVEDLFPSSPTWWDDLKTRALTEKKDLLELNELNLELKNED"
     gene            972..1328
                     /locus_tag="ACHKBL_004977"
     CDS             972..1328
                     /locus_tag="ACHKBL_004977"
                     /inference="COORDINATES: protein motif:HMM:NF045244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5821696.1"
                     /translation="MKIKLFALLIFLPIISFAKEPHVCNSSMQSHPDIIDCIQISTRK
                     IEDSIKEIIAKSGNEYSIDMAFYNSQRKSISERCSLYYSMEGQRIDLIENQCEYDGVE
                     QLNKFIKTYMKTVDNY"
BASE COUNT          662 a          296 c          379 g          584 t
ORIGIN      
        1 cgatgatacg cgaaccagtc atgcgctggt gaaacataat tatcagcgcc tgctggataa
       61 aattgatagt ggaagcgacc gttactctcc aatggaatac tggagagcac ttcataaccc
      121 cgactatcgt gatgttcttc agaaaacaat cgttaaacat cccagcgact ggtacttcaa
      181 aaaagacgat gcaatctggc aaccatttct gaatgcgctg aaaaaagatg ctccggaatg
      241 gaagaaatac agcgaagatt ttcttgataa gatggcctgg atgcaggatg tgaccacgga
      301 gaaactgggc cctttgttgt ggcatatgca tccgattatg ttcttaggct gtatgaaaaa
      361 atcgtcagga atatggccac ttgggaaaac ttcagagcgt tatgagtctg gaggaagagg
      421 gccaggtgta atttcatctg gaaaaggaga tcatggcggt gtgtcatatg gaatgtatca
      481 attatcttct aagatgggaa cggttaataa atatattata atgtcaaaat acaaagattt
      541 atttgagggt aaaaaacctg ctacacctga attcaatgaa atatggaaaa aaattgctct
      601 cattcacaaa gaagaattcg cacaagatca gcatcttttt ataaaaaaaa cacactatca
      661 agtacaatta gactatttgg catccaaagg gctaaatttc tctcattctc ttgccgcaat
      721 caatgatatg atttggtcga cttccgttca atatggccct acgactggtt taatttcgag
      781 agcgttgaaa ggaaagaatt tagaaacatt aacagatgca gatgtgataa ctattgttca
      841 ggactacaaa ataaatcatg ttgaagatct ttttccatca tccccgactt ggtgggatga
      901 tttaaagaca cgagcgctaa cagaaaagaa agatttattg gaattaaatg aattgaatct
      961 ggagttaaag aatgaagatt aaattatttg cattgcttat tttcttgccg attatctctt
     1021 ttgcgaaaga accacacgtt tgcaattcct cgatgcagtc tcatccagat attatcgatt
     1081 gcatacaaat tagcacaagg aaaatagagg attccattaa agaaataatt gcgaaatctg
     1141 gcaacgagta ttcaattgat atggcgtttt ataatagcca gagaaagtct atttctgaac
     1201 gatgcagctt atattattcg atggaaggtc aacgaattga tttgatagaa aatcaatgcg
     1261 aatatgatgg tgtagaacaa ctaaataaat ttattaaaac ctatatgaag acggttgata
     1321 attattaaaa tcaaactgtc tttggttatg ttaattgtta atggtattaa caatatatgc
     1381 tcaatgctct caaagaaaaa tattgacagt tctaatcctc aattttctga ctacttggtt
     1441 tccgtaatta acaggtcatt tagaagtatc tcaataagca agagttatag gtctgtatgt
     1501 gaagtggcct tatttaattt taattatagt aatggtgtaa aattaatagg attgctggac
     1561 ggggttaata attaatgact gtctcataaa tgttcatcat aacgagaaaa cggcagtagt
     1621 ataattcgga tgcaacatgc atctggagac gttaagggtt gagttaatag atttgactct
     1681 aaatccttga taaatattaa cttagactaa acaaatgggc ttgaaaaaac attgctattg
     1741 ctctgcactt tatttgcatt tctcatatgt gataattttg ctgttacaaa tgaaatgcca
     1801 agggaaaata ttcccttagg aaaaatgttt gtggtataaa aattaatgac gaacttgtag
     1861 agatgtagat agttctaaat gtgaatttta ttgcgcacta tctgtagtat cgcattcata
     1921 t
//
LOCUS       DAZFLD010000424         1905 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.426, whole genome shotgun sequence.
ACCESSION   DAZFLD010000424 DAZFLD010000000
VERSION     DAZFLD010000424.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1905
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.426"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(81..1493)
                     /gene="pykF"
                     /locus_tag="ACHKBL_004978"
     CDS             complement(81..1493)
                     /gene="pykF"
                     /locus_tag="ACHKBL_004978"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase PykF"
                     /protein_id="HFO5821697.1"
                     /translation="MKKTKIVCTIGPKTESEEMLAKMLDAGMNVMRLNFSHGDYAEHG
                     QRIQNLRNVMSKTGKTAAILLDTKGPEIRTMKLEGGNDVSLKAGQTFTFTTDKSVIGN
                     SEMVAVTYEGFTTDLSVGNTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNL
                     PGVSIALPALAEKDKQDLIFGCEQGVDFVAASFIRKRSDVIEIREHLKAHGGENIHII
                     SKIENQEGLNNFDEILEASDGIMVARGDLGVEIPVEEVIFAQKMMIEKCIRARKVVIT
                     ATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKYPLEAVSIMATICER
                     TDRVMNSRLEFNNDNRKLRITEAVCRGAVETAEKLDAPLIVVATQGGKSARAVRKYFP
                     DATILALTTNEKTAHQLVLSKGVVPQLVKEITSTDDFYRLGKELALQSGLAHKGDVVV
                     MVSGALVPSGTTNTASVHVL"
     gene            complement(1501..1695)
                     /locus_tag="ACHKBL_004979"
     CDS             complement(1501..1695)
                     /locus_tag="ACHKBL_004979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5821698.1"
                     /translation="MDCRTQRLKNRHSRWAETQAHIPLHAFSMSPILRARHHHFRNTG
                     FAFRQCAQKASFSHPSQLKD"
BASE COUNT          476 a          444 c          472 g          513 t
ORIGIN      
        1 tattgcttct ggttatcgat taaataaaaa aagcgcccat cagggcgctt cgatatacaa
       61 attaattcac aaaagcaata ttacaggacg tgaacagatg cggtgttagt agtgccgctc
      121 ggtaccagtg caccagaaac cataactacg acgtcacctt tgtgtgccag accgctctgc
      181 agagccagtt ctttacccag acggtagaaa tcatcagtag aagtgatctc tttaaccagc
      241 tgcggcacaa cgcctttgct cagtaccaac tgatgagccg ttttttcgtt ggtggtcagt
      301 gccaggatgg tggcatccgg gaagtattta cgtactgcgc gagcagattt accgccctgg
      361 gtagcaacca cgatcagcgg ggcatccagt ttttcagcag tttcaacggc accacggcat
      421 accgcttcgg taatgcgcag tttacggttg tcattgttga actcgagacg gctgttcatc
      481 acgcggtcgg tacgttcgca gatggtcgcc atgatagaaa ccgcttccag cgggtattta
      541 ccttttgcgg attcaccaga cagcatcact gcgtcagtac cgtcgaggat ggcgtttgca
      601 acgtcaccgg cttctgcgcg agtcgggcgt gggtttttaa tcatggaatc cagcatctgg
      661 gtcgcagtga taacgacttt acgtgcacgg atacattttt cgatcatcat cttctgggcg
      721 aagataactt cttctaccgg gatttctaca cccaggtcgc cacgcgcaac catgatgccg
      781 tcagaggctt cgaggatttc gtcgaagttg ttgaggcctt cctggttttc gattttggag
      841 atgatgtgga tgttttcgcc gccgtgcgct ttcaggtgct cacggatttc gataacgtca
      901 gaacgcttac gaataaagga agcagcaaca aagtctacgc cttgttcgca accaaagatc
      961 aggtcctgtt tgtctttttc agccagtgct ggcagggcaa tggaaacgcc aggcaggttc
     1021 acacctttgt tttcacccag gtcgccgttg ttcagcactt tacagataac tttgttacct
     1081 tcaatggcgg taacttccat accgatgaga ccatcgtcaa ccagtacggt gttgccaaca
     1141 gacaggtcag tagtgaaacc ttcatacgtt accgcaacca tttcgctgtt gccgataaca
     1201 gatttatcag tggtgaaagt aaaggtctga cccgctttca gagaaacgtc gttaccgcct
     1261 tccagtttca tggtgcggat ttccggacct ttggtatcaa gcaggatagc ggcggtttta
     1321 ccagttttgc tcatcacgtt gcgcagattc tgaatgcgct gaccgtgttc tgcatagtca
     1381 ccatgagaga agttcagacg cataacgttc atgccagcgt ccagcatttt agctaacatc
     1441 tcttcagatt cggttttcgg tccgatggtg caaacaattt tggtcttttt catgacagtc
     1501 ttagtcttta agttgagaag gatgggagaa acttgctttc tgggcgcact gccggaaagc
     1561 gaatccggta ttacgaaagt ggtggtgcct cgctctaagg ataggtgaca tcgaaaaagc
     1621 gtgcagagga atgtgtgctt gtgtctcagc ccaacgtgag tgcctgtttt tgagtcgttg
     1681 agttctacag tccaaagtgc tgaaaccatt caagagtcaa ttggcgcgca ttatacgctg
     1741 aaattaatcc aaaaggaaaa caaaaacagc gtttcaaaaa atatacaact tgttagacaa
     1801 taaattgtta tcaaagattt aacgttccag ggaaaaggtt acgcaaaaac ttcgctgttt
     1861 gcgcaacgtc tggtgccggg gttgtcactg tgaagataag tgaaa
//
LOCUS       DAZFLD010000425         1899 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.427, whole genome shotgun sequence.
ACCESSION   DAZFLD010000425 DAZFLD010000000
VERSION     DAZFLD010000425.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1899
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.427"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1126
                     /locus_tag="ACHKBL_004980"
     CDS             <1..1126
                     /locus_tag="ACHKBL_004980"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004168096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFO5821699.1"
                     /translation="PAIFSFFSGSGFLDLGFENAGYEVVFVNEFHPPFMDAYKHSRKK
                     LGKQPPRFGYAQESIETIEHSVIKHSMKTLKGENRLVGFIGGPPCPDFSVAGKNKGKD
                     GENGKLSQSYIDLIIEMMPDFFLFENVKGLYRTLKHREFFEQIKNELASVGYILHERL
                     INSIEYGAPQDRERIILVGFLDKSLAKNFEWSGQIYPNKEAFQFPWPTTNTYEEDSSM
                     ECPQGIPEELTIEYWFEKNDVTNHPNAKHVFTARAGLARFQSVEEGDDSKKSYKRLHR
                     WRYSPTAAYGNNEVHLHPYKPRRITVAEALAIQSLPKNFELPGDMTLSNMFKTVGNGV
                     PYIAALGIAQSILQHLGYSNEDNSIESGSGDFELTQKYAV"
     gene            1062..>1899
                     /locus_tag="ACHKBL_004981"
     CDS             1062..>1899
                     /locus_tag="ACHKBL_004981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004171733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HFO5821700.1"
                     /translation="MRITASNLVQAISSLPKNTLFNYINDRNSGKIEIVRIQHPEGPI
                     EIKRFDPKKGQTQATAKTESISTQMLWRLANALEENRPVNVERVFGASYNTRSVLESL
                     LAHTPEFYWCRPARLEIMNAQKSIKPGHKHLIFLPDMPHTNGLLIEHQTNIVVSEMSF
                     DVVHQSVDIETIKPTKGMTIEEKRRHAQIQIALVKIGYCLGLDTWVAANDRSLQYNGK
                     AIAQMDGVISSLSDEQVLQSYNDAIKDARLIDCIWFREGRMMPAVMEIEHSTGIKSGL
                     TRM"
BASE COUNT          626 a          371 c          397 g          505 t
ORIGIN      
        1 acccgctata ttttcattct tttcaggatc aggttttctt gacctaggct tcgaaaatgc
       61 cgggtatgag gtggttttcg tcaatgaatt ccatcctccc ttcatggacg cgtataaaca
      121 ctcaagaaaa aaacttggca aacagccgcc acgctttggg tatgcccaag aaagcattga
      181 aactattgag cattcagtga ttaagcatag catgaaaaca ctgaaaggtg aaaaccgcct
      241 agtgggcttc attggcggac caccatgccc tgacttttct gttgctggca aaaacaaagg
      301 caaagatggt gaaaatggca aattatcaca aagttacatt gacctgataa ttgaaatgat
      361 gcctgatttc ttcctttttg aaaacgttaa aggcttatac cggactctta aacatagaga
      421 atttttcgaa caaattaaga atgaactagc ttcagtagga tatatcctgc atgaaagatt
      481 gattaattca attgaatacg gtgcgcctca agatcgagaa aggattattt tagttggttt
      541 tcttgataag tctttagcca aaaacttcga atggagtggc caaatatacc ccaataagga
      601 agctttccag ttcccttggc caacaacaaa cacatacgaa gaagactcaa gtatggaatg
      661 cccgcaaggt attccagaag aacttactat agaatactgg ttcgagaaaa acgatgttac
      721 caatcatcca aacgctaaac atgtatttac ggcccgagcc ggtttagcaa gattccagtc
      781 agtagaagaa ggcgacgata gtaaaaaatc atataaaagg cttcatcgct ggagatactc
      841 tccaacagca gcatatggta ataatgaagt tcaccttcac ccatacaaac ctcgtcgtat
      901 tacggtcgct gaagcattgg caatccagtc tttgccaaaa aattttgagc taccagggga
      961 tatgactttg tcgaatatgt ttaaaacagt cggaaacgga gttccctata tcgccgcgct
     1021 cggtattgcc caatcaattt tgcaacattt aggatacagt aatgaggata acagcatcga
     1081 atctggttca ggcgatttcg agcttaccca aaaatacgct gtttaactat ataaacgata
     1141 ggaatagcgg gaagattgag attgtacgta tccaacatcc cgaaggtcct atcgaaataa
     1201 agagatttga tcctaagaaa ggtcaaactc aagcaacagc taaaacagag agtatttcga
     1261 ctcaaatgct ttggcgactt gcaaatgcac tcgaagaaaa cagacctgtc aacgttgaaa
     1321 gggttttcgg tgccagttac aacacccgct cggtattgga gagcttgctc gcacatactc
     1381 cagagttcta ttggtgtagg ccagccagac tggaaatcat gaatgcacaa aagtctataa
     1441 aaccaggaca taagcatctg atttttctgc cagatatgcc acatactaat ggtttgttaa
     1501 ttgagcacca gactaatatt gttgtctctg aaatgagctt cgatgttgtt caccaaagcg
     1561 ttgatattga gacaattaaa ccgactaaag gcatgactat tgaagaaaaa agacgtcatg
     1621 ctcagataca aattgcctta gtaaaaatag gttattgttt aggtttagat acttgggtag
     1681 cagccaatga tcgtagctta caatataatg gtaaagctat agcgcaaatg gacggtgtaa
     1741 taagttcatt atctgatgag caagtactcc aatcgtataa tgatgcgatt aaagatgctc
     1801 gtcttataga ctgcatttgg tttagggaag gccgcatgat gccagcagta atggagattg
     1861 aacacagtac tggtataaaa agtggtctga ctcgcatga
//
LOCUS       DAZFLD010000426         1891 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.428, whole genome shotgun sequence.
ACCESSION   DAZFLD010000426 DAZFLD010000000
VERSION     DAZFLD010000426.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1891
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.428"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            470..663
                     /locus_tag="ACHKBL_004982"
                     /pseudo
     CDS             470..663
                     /locus_tag="ACHKBL_004982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080195.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase zinc-finger binding
                     domain-containing protein"
     gene            761..1498
                     /locus_tag="ACHKBL_004983"
     CDS             761..1498
                     /locus_tag="ACHKBL_004983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000634445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFO5821701.1"
                     /translation="MIVCYVILVNRPFETEIAGKKLFFQKNDVLLTDNISQSFFLRHP
                     GNVTELRIDECIVRKYMESADRIQHSDSAVSVPFIKTTFSHIALLHNVMQNLSLQKTL
                     PQQFSEQMFFACLSIFSTHRGFMFLLFRCINSVALKVRYIICSSPSRAWRVKDISNSL
                     YMSESLLKRKLQEEKVSFSQILLDIRMHNARQLISSKLSVNQIAIRCGYASTPYFISV
                     FREYFGITPHQMMINQSGFIESRIAIK"
BASE COUNT          552 a          393 c          405 g          541 t
ORIGIN      
        1 tttacctttt acaaaaaatg aatattactg acaaaactaa atataaataa tgactgcctc
       61 ggcatgccag ttaacagcca tccgcaatgg cgtgttcggt tttgcgaata aaaaatggtt
      121 actgcagaag cggattttca ccacctgtca accatgaatg gtcagtggag tgagacgact
      181 ggtatgattt ggtcgatgac ttcttccggt gaatacggac agaaacattg aaatattgta
      241 agagagaacg ttcagaattc tgtgtcacgt gaaaatacta cagcgttatc accttattca
      301 gtcgaccttc aaaataaacg tctaaccgtg acaatcacca tttggattgc ccactgggga
      361 ggcgaacgat tgcctcgacg gcatttataa tgccagccca cattgttggc gctgacaata
      421 ctaaggacaa acgatgaggg agaaccgtct tataccacta gcgctgacac cggcacacct
      481 tccccgtgaa atacaccggc tggagcctga agaaagctgt tgcccggagt gtggcggtga
      541 gctggattat ctggggaagt cagcgctgaa caactggaac tggtgagcag ctccctgaaa
      601 gtgatcagca cagaacgggt aaaaaaagcc tgtaaaaaat gtgacggtac cgttgaagcg
      661 ccggtctcgt aagaataatc ctggacaaat aagatttttc ctgaaaaagc gtctagtctt
      721 ttgatatcgt tgtttttatg ctaatcaggg agtatgtgtg atgattgtct gttacgttat
      781 tcttgttaac cgtccgtttg aaacagagat tgccgggaaa aagctatttt ttcagaaaaa
      841 tgacgtcctg ttgacagata acatcagcca gtcctttttc ctgcgccatc ccggtaatgt
      901 tactgagctc cggattgacg aatgcattgt cagaaaatat atggaaagtg ccgacaggat
      961 ccagcactct gatagtgctg tttctgttcc gttcattaaa acaaccttct ctcacatcgc
     1021 tctgttacat aatgtcatgc agaatctcag tctgcaaaag acattgccac aacaattttc
     1081 tgagcagatg ttttttgcct gcctttccat tttttccaca cacagaggtt tcatgtttct
     1141 gctattcaga tgtataaact ctgttgcgct taaagtcagg tatattatct gttccagtcc
     1201 atcacgggca tggagagtta aagatatcag taattctctg tacatgagtg agagtttact
     1261 gaaaagaaaa cttcaggagg aaaaagtttc tttctcccaa atcctgctgg atatacgcat
     1321 gcataacgcc cgacaactta tttccagtaa actgtcagtg aatcaaatcg caatacgttg
     1381 cggttatgcc agcacaccgt attttatcag cgttttcaga gaatattttg gtataacacc
     1441 tcatcagatg atgattaatc agtccggatt tattgagagc agaatcgcaa ttaagtaaag
     1501 tatgtcagtc agcgcataat aaaagtattc ttatgcttca ttatcagttt ctgtttatca
     1561 ctccggagca ctgtagtaac tacggacccg gaaaacagat ggtaatgagt tgactgactg
     1621 atgatattgt ggcgacattt ttacggatgc gagcaaagaa tgtattggtc cgtttgatta
     1681 tgcagtgatt aacaaaactc cgggaagatg aatgagtata cctctcctgc aatccgtttc
     1741 ctgagaaaac aggtggtaac acaatattct taatatgtta ctgtttcacc acacaaccag
     1801 gtttacggtt gtgtggtgaa ctatagtaat cccgggtaac aatccggagc tgttcctttt
     1861 atactcttca gactgtgtac tcatccacgc c
//
LOCUS       DAZFLD010000427         1880 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.429, whole genome shotgun sequence.
ACCESSION   DAZFLD010000427 DAZFLD010000000
VERSION     DAZFLD010000427.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1880)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1880)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1880
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.429"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..285
                     /locus_tag="ACHKBL_004984"
     CDS             <1..285
                     /locus_tag="ACHKBL_004984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5821702.1"
                     /translation="IICLLCVVATFCVSVILPESHLKHLLFSCTILCMVCSFIIYYYT
                     RHMVLNRQELLRLMRLTEGAPDVKQELLNRLLSGKILTYWDEKDIRRLIF"
     gene            298..504
                     /locus_tag="ACHKBL_004985"
     CDS             298..504
                     /locus_tag="ACHKBL_004985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5821703.1"
                     /translation="MSEIRFVTLSSDTDINKIIQEGLTQKDATTTIVLGCASEGKEAP
                     LRLIETCFRTIPKHISIEEINEIK"
     gene            621..1196
                     /locus_tag="ACHKBL_004986"
     CDS             621..1196
                     /locus_tag="ACHKBL_004986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012908955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFO5821704.1"
                     /translation="MQYALFDGFERKFLLDALEFGVLKDWKEYPVKELPDIDESAHPF
                     YVCYGGYLLNPSVSDSDISRKIKDQTGFWLAAIDDTHMDCHSIAYYDIHTLPLISCGR
                     QKIVPFAALIKADRCIISKIASYSGFAVTAFLRIKEWDIATNILNREGIFAFNGCEHR
                     FRQPVGEDNWKQAISEECAIRYANRLIQCEG"
     gene            1275..1787
                     /locus_tag="ACHKBL_004987"
     CDS             1275..1787
                     /locus_tag="ACHKBL_004987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HFO5821705.1"
                     /translation="MKITLTIFIFLLTAFQAANAGGIPGALAGILSTLNLEIQGKVIR
                     VLDGDTIEVLQDKKPVRIRLANIDAPEKKQAFGRWSANQLKALLAAQPVTVTYTQTDR
                     YGRIIGRVFTTNGTDASRFMVQSGAAWGYERYNTDKSLPALQREAQEQKRGLWADTNP
                     VPPWEWRKNN"
BASE COUNT          553 a          340 c          428 g          559 t
ORIGIN      
        1 attatatgtc ttctttgtgt tgttgccacg ttctgtgttt ctgttatatt gccggaatca
       61 catctgaaac acctgttatt ttcctgtact atcctttgta tggtatgcag ttttattatt
      121 tattattaca cccgccatat ggtcctgaac cgtcaggaac tgcttcgact tatgcgtctg
      181 acagagggcg caccagatgt gaaacaggaa ctgcttaacc gtctgctatc cgggaaaata
      241 ctgacttatt gggatgaaaa agatatccga cgtctaattt tttgaagagg taatcatatg
      301 agcgaaatca gatttgtaac tctgtcatct gacactgaca ttaacaagat tattcaggaa
      361 ggtcttactc aaaaagatgc tacaactacg attgtgttgg gttgcgcgag tgagggtaaa
      421 gaagcaccgc tcaggctcat agagacttgt ttcaggacta taccaaagca tatttcaatt
      481 gaagaaatca atgaaataaa atgaatgatg acggtagtcg ttgttattct ggtattatcg
      541 aatctcatta tatttaagag acagccatct attgatagcg cattagttat ataaatcagg
      601 cattctgtga ggggtagtat atgcaatatg cattgtttga tggatttgaa cgaaagtttt
      661 tgctggatgc tcttgagttc ggtgttctga aggactggaa agaatatccg gtaaaagagc
      721 ttcctgatat tgatgaatct gctcacccat tctatgtctg ttatggtgga tatttattaa
      781 atcctagtgt ttcagattct gatattagca gaaaaataaa ggaccagaca ggattctggc
      841 tggcagctat tgatgatact cacatggatt gccattcaat agcttattat gatattcaca
      901 cactccctct gatttcgtgt ggacgtcaaa agatagttcc ttttgccgcg ttaataaaag
      961 ctgacagatg catcatttcg aaaattgcat catattctgg ttttgcagta acggcatttt
     1021 taaggattaa agagtgggat attgcaacca acatacttaa tcgcgagggg atttttgctt
     1081 ttaatggctg tgaacacagg ttcagacaac ctgtaggtga agataactgg aaacaggcaa
     1141 tatcagaaga atgtgccatt cgctatgcaa acagattaat tcaatgtgaa ggataagcaa
     1201 atcataaact ccgcagattc ctgattttaa tatgggattt gcggagttga acaaccggag
     1261 ggggttagta ttttgtgaaa attacattaa caatatttat ttttctgctt actgcctttc
     1321 aggctgcaaa tgcaggtgga atacctggtg ctttagcagg aatactttct acattaaacc
     1381 tggaaatcca ggggaaagtt attcgtgttc tcgatggcga taccatcgaa gttttacagg
     1441 acaaaaagcc cgtcaggatt cgcctggcaa acatcgatgc accggagaaa aaacaggctt
     1501 tcgggcgctg gtcagcaaat cagcttaaag ctctgcttgc agcacagcct gttactgtga
     1561 cgtacacgca gacagaccgc tacggacgca ttatcggtcg tgtgttcacg acgaacggca
     1621 cggatgccag ccgctttatg gttcagtctg gcgctgcatg gggatatgaa cgttacaaca
     1681 cagataaatc attaccggct ttgcaacggg aagcgcagga acaaaagcgt ggtttatggg
     1741 ctgatacaaa tccggttcca ccgtgggaat ggcggaaaaa caactgattt attaaaggta
     1801 acatatgggg aaattaactt tggtagagca agctttgcgg cttcataaag aatattatgg
     1861 tgatgaatgt tttggcacta
//
LOCUS       DAZFLD010000428         1879 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.430, whole genome shotgun sequence.
ACCESSION   DAZFLD010000428 DAZFLD010000000
VERSION     DAZFLD010000428.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1879
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.430"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(168..821)
                     /gene="ribB"
                     /locus_tag="ACHKBL_004988"
     CDS             complement(168..821)
                     /gene="ribB"
                     /locus_tag="ACHKBL_004988"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HFO5821706.1"
                     /translation="MNQTLLSSFGTPFERVENALVALREGRGVMVLDDEDRENEGDMI
                     FPAETMTVEQMALTIRHGSGIVCLCITEDRRKQLDLPMMVENNTSAYGTGFTVTIEAA
                     EGVTTGVSAADRITTVRAAIADGAKPSDLNRPGHVFPLRAQAGGVLTRGGHTEATIDL
                     MTLAGFKPAGVLCELTNDDGTMARAPECIEFANKHNMALVTIEDLVAYRQAHERKAS"
     regulatory      complement(925..1073)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            1195..1494
                     /gene="ubiK"
                     /locus_tag="ACHKBL_004989"
     CDS             1195..1494
                     /gene="ubiK"
                     /locus_tag="ACHKBL_004989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311957.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis accessory factor UbiK"
                     /protein_id="HFO5821707.1"
                     /translation="MIDPKKIEQIARQVHESMPKGIREFGEDVEKKIRQTLQAQLTRL
                     DLVSREEFDVQTQVLLRTREKLALLEQRISELEARNNSVADLQSPPAIPPIDKAE"
     gene            complement(1529..1729)
                     /gene="glgS"
                     /locus_tag="ACHKBL_004990"
     CDS             complement(1529..1729)
                     /gene="glgS"
                     /locus_tag="ACHKBL_004990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface composition regulator GlgS"
                     /protein_id="HFO5821708.1"
                     /translation="MDHSLNSLNNFDFLARSFARMHAEGRPVDILAVTGNMDEEHRTW
                     FCARYAWYCQQMMQTRELELEH"
BASE COUNT          539 a          475 c          414 g          451 t
ORIGIN      
        1 aacaattata tgattttgat cataatgaaa aagaatgcct gatatcaatg aaattttctc
       61 atagaaagag aagcggaatt aacatcttgc atagcagggc aaaataaaaa ccgaagccag
      121 ctacaacttc ggtttcttga ttaagacagt aaattaagca gcgattttca gctggctttg
      181 cgttcatgcg cctgacgata agccaccagg tcttcaatag tcacgagcgc catattgtgt
      241 ttattggcaa actcaatgca ctctggtgca cgcgccatcg tgccatcgtc attagtcagc
      301 tcacacagca caccagccgg tttaaagcca gccagcgtca tcagatcaat agtcgcttca
      361 gtatggccgc cacgcgtcag tacaccacca gcctgagcgc gaagtgggaa aacgtggccc
      421 gggcgattca gatcagaagg ttttgcgcca tcggcaatcg ctgcgcgaac ggtcgtaata
      481 cggtcagcgg cagaaacacc ggtagtcaca ccttcagctg cttcaatggt cacggtaaaa
      541 ccggtgccat aggcgctggt gttattttct accatcattg gcagatcgag ttgtttacgg
      601 cgatcttcag tgatgcacag gcaaacaata ccgctaccgt ggcgaatggt cagcgccatc
      661 tgctcaacgg tcatggtttc tgccgggaag atcatatcac cttcgttttc acggtcttca
      721 tcatcaagca ccattacacc gcgtccttca cgcagcgcaa ccagtgcatt ttcaacacgt
      781 tcgaaaggcg taccaaaaga ggaaagtagc gtctgattca tggtaaaaaa acctcactaa
      841 aattatggtt accagaatca gggcagtctt aggagtggcg gcatatagcc aaaataacgt
      901 gagcgggtcc atgcccgaca gaatcgttac tctctcccat ccggactcta accgtcggcc
      961 ccggaattac accggatctg ctgtcctttg agttcgcacc caaagcgctc gcgggctttc
     1021 aactgagttg atttaccgcc ggtggggaat ttcgccccgc cctgagaata agcggattca
     1081 ctataacgct aatgattagc ggcagcaacg catagcttca cataattctg gtttatgact
     1141 tacccttatc gcactacaat ggcactcaac acctatcagt acagggaaac tacaatgatt
     1201 gacccgaaaa aaattgagca aatcgctcgc caggttcacg aatcaatgcc taaaggaatc
     1261 agggagttcg gggaagatgt ggagaaaaaa atccgccaaa ccctacaagc gcagctgacg
     1321 cgtctcgatc tggtaagccg cgaagaattc gacgttcaga cgcaagtcct gttacggact
     1381 cgtgaaaaac tggcgttact ggagcagcgc atcagtgaac tggaagcgcg caataattcg
     1441 gtagcagatc ttcaatctcc tccggctatc ccgcctattg ataaagcaga ataaagcatc
     1501 acaaaaacgg gccagacggc ccgtaatatc agtgttctaa ttcaagttct ctggtttgca
     1561 tcatctgttg gcaataccag gcataacgtg cgcaaaacca ggttctatgt tcttcatcca
     1621 tgttaccagt aacggccaga atatcgaccg ggcgaccttc tgcgtgcatt ctggcaaaac
     1681 tacgcgccag gaaatcgaaa ttatttaaag aattaagact atgatccata agcacatcct
     1741 ccggttatcg catacctttt aaggccatat gctttttctg atttcagtat tccccaatca
     1801 gtgtagtgcc tttaaacata acgtgcgtaa atatattgtc cccgatcaca ctttttagtg
     1861 aaaaataaaa aaccatatt
//
LOCUS       DAZFLD010000429         1863 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.431, whole genome shotgun sequence.
ACCESSION   DAZFLD010000429 DAZFLD010000000
VERSION     DAZFLD010000429.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1863
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.431"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..542)
                     /locus_tag="ACHKBL_004991"
     CDS             complement(<1..542)
                     /locus_tag="ACHKBL_004991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000203832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antA/AntB antirepressor family protein"
                     /protein_id="HFO5821709.1"
                     /translation="MANQLIPVFNGTIANETTLLVNARDLHTFLGVGKRFASWITERI
                     AEYGFVENQDYILVSLNRETKGRGGDRRSKDYHLTLDTAKETAMVERNEKGRQIRRYF
                     IECEKKFRQSLLPAPMNINYPLSWFSDNHPYATMNYVDRKVISLDASVLFDMPSPTMR
                     ILNELHSKGYNVDAAVAEFNA"
     gene            complement(793..1440)
                     /locus_tag="ACHKBL_004992"
     CDS             complement(793..1440)
                     /locus_tag="ACHKBL_004992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000211992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFO5821710.1"
                     /translation="MATIPVLSQPEITISNGRAVTTSFAVAEYFCKRHDNVLRAIANI
                     ECSEKFNALNFEDVTYTDAKGEQRPMYQITKNGFVFLVMGFTGKKAAAFKEAYIAEFD
                     RMEKQLWEQKQLALSSPAMNINFPLSWFSEHHPYATMNYVDRNTLQLDTSVLFDMPSP
                     AMRIFNELRRNGYNVDAAVAEFNAFKHLTEEMRRTLQDISRLSDRNSRKGFSLSL"
BASE COUNT          460 a          413 c          488 g          502 t
ORIGIN      
        1 gcgttaaatt cggcgacagc ggcgtcaacg ttatagcctt tgctgtgtag ctcattgagg
       61 atgcgcatag ttgggcttgg catatcgaag agcacggaag catcaaggct gataactttg
      121 cgatcaacat agttcatcgt ggcgtaggga tggttatctg agaaccacga gagggggtag
      181 ttgatgttca ttggtgcagg taaaaggctc tggcgaaact ttttctcgca ctcgatgaag
      241 tatcggcgga tctggcggcc tttttcgtta cgttcaacca tcgcagtttc tttggctgta
      301 tcgagggtga ggtgatagtc tttgctacgg cgatcgcctc ctcgaccttt ggtttcccga
      361 tttagggaaa ccaaaatata gtcctgattt tcaacgaaac catattcagc aatgcgttct
      421 gtaatccacg atgcaaagcg tttacctacc ccaagaaaag tatgtaaatc gcgagcatta
      481 acgagaagag tggtttcgtt ggcgatagtg ccgttgaata cggggatgag ttgattagcc
      541 ataattatga ctcctgacgt tttagtattg actgccacct tagtggggtg gcgggcttca
      601 actaccgcgt cagacggcgg agcgtatttc ccgaaagggt attgtattag gctctctcga
      661 cccgccattg atatggcgat acctgtaaac aggcataaaa aagccgcaaa gctatcgggt
      721 gcggttgacc gctgacgttg tagtgcggtc agtatgcgat agctttggcg gcattgtcaa
      781 acgatctgtt ctttaaagac ttaacgagaa gccttttcgg gaatttcgat ctgacagtct
      841 tgaaatatcc tgtagcgtgc ggcgcatttc ttccgtcaga tgtttgaagg cgttaaattc
      901 ggcgacagcc gcatcgacgt tatagccgtt acgacgtaat tcattaaata tgcgcattgc
      961 agggcttggc atgtcgaata agacggaggt atcaagctgc aatgtatttc tatcaacata
     1021 gttcatcgtg gcgtaggggt gatgctctga aaaccacgat aaagggaaat tgatattcat
     1081 cgcaggagaa gacagggcca gttgtttctg ttcccatagt tgtttttcca tgcgatcgaa
     1141 ttcagcaatg taggcttcct tgaatgcagc ggctttttta ccagtgaatc ccatcaccag
     1201 gaaaacgaat ccgtttttgg tgatttggta cattgggcgt tgttcgcctt tggcgtcggt
     1261 gtaggtaacg tcctcaaaat tgagggcgtt aaatttctct gagcattcaa tatttgcaat
     1321 ggcacgtaat acgttatcgt gtcgcttgca aaaatattcg gcaacggcaa aggacgtagt
     1381 aacagcgcgg ccattggaaa tggtgatttc aggttgagaa aggactggga tagtagccat
     1441 aatggcagcc tccttgattg gtgattaata accaccgctg gaggtgagaa gctcgctggc
     1501 ggtggactgt acagggttct cacaactggc aatcaaggga accagcccga ccgaagtcgg
     1561 ccccatacag cccaccattg ataagatgtg cgtgtatgtt gatacaaaaa aagacgctgg
     1621 cgcgtctgta tcgcctcgat tgtcagcggg gtgagaatcc cgacacccgt tttataaggt
     1681 gttcgccaaa tatagccccg acatcacacg cagtcaatac cgtcctttct cagaaacgct
     1741 ttggcgatgt ggtaggtggg agacccattt cgacccgatt cggcctactt atcttcagca
     1801 atagtccctc gggcctcgcc gctttacgtg cgacatattc ccgtccatga acccttcacc
     1861 aca
//
LOCUS       DAZFLD010000430         1846 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.432, whole genome shotgun sequence.
ACCESSION   DAZFLD010000430 DAZFLD010000000
VERSION     DAZFLD010000430.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1846
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.432"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            156..659
                     /locus_tag="ACHKBL_004993"
     CDS             156..659
                     /locus_tag="ACHKBL_004993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin-like protein"
                     /protein_id="HFO5821711.1"
                     /translation="MATAGMLLKLNSQMNREFYASNLYLHLSNWCSEQSLNGTATFLR
                     AQAQSNVTQMMRMFNFMKSVGATPIVKAIDVPGEKLNSLEELFQKTMEEYEQRSSTLA
                     QLADEAKELNDDSTVNFLRDLEKEQQHDGLLLQTILDEVRSAKLAGMCPVQTDQHVLN
                     VVSHQLH"
     gene            complement(737..988)
                     /gene="yecJ"
                     /locus_tag="ACHKBL_004994"
     CDS             complement(737..988)
                     /gene="yecJ"
                     /locus_tag="ACHKBL_004994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2766 family protein YecJ"
                     /protein_id="HFO5821712.1"
                     /translation="MSQPLNADQELVSDVVACQLVIKQILDVLDVIAPVEVREKMSSQ
                     LKNIDFTNHPAAADPVTMRAIQKAIALIELKFTPQGESH"
     gene            complement(1103..1189)
                     /gene="azuC"
                     /locus_tag="ACHKBL_004995"
     CDS             complement(1103..1189)
                     /gene="azuC"
                     /locus_tag="ACHKBL_004995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein AzuC"
                     /protein_id="HFO5821713.1"
                     /translation="MKLRKILKSMFNNYCKTFKDVPPGNMFR"
     gene            1452..1775
                     /gene="yecR"
                     /locus_tag="ACHKBL_004996"
     CDS             1452..1775
                     /gene="yecR"
                     /locus_tag="ACHKBL_004996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecR-like lipofamily protein"
                     /protein_id="HFO5821714.1"
                     /translation="MRLLILTLSLITLAGCTVTRQAHVSEVDAATGIVRLVYDQAFLQ
                     HARTDRYVSRGIADRACQQAGYTHAIPFGQPVGNCSLFAGSLCLNTEFTLSYQCHHSA
                     FPVFL"
BASE COUNT          467 a          436 c          415 g          528 t
ORIGIN      
        1 tacccgttca gacgttattc ttatttcaga tcatcgtcag aattgactcc acgatcacat
       61 ttcggaccgg cagaaaggaa ttattctgca aacagtaatt atggtgtttt gatttatctt
      121 gcacctctcc acttctggat ataaggatat taggtatggc aaccgctgga atgcttctca
      181 aactcaactc tcaaatgaac cgcgagtttt acgcatccaa tctctacctt cacctgagta
      241 actggtgttc tgaacagagt ctgaacggca ccgccacttt ccttcgcgcc caggcacaga
      301 gtaatgtgac ccaaatgatg cgcatgttta actttatgaa gagtgtcggc gctaccccca
      361 tcgttaaagc cattgatgtt cccggtgaaa aactgaactc tctggaagaa ctgttccaaa
      421 aaacgatgga agaatacgag caacgttcta gtacgttggc acagttagcc gatgaagcga
      481 aagaactgaa tgatgattca accgtcaatt tcctgcgcga tctggaaaaa gaacagcagc
      541 atgatggcct gttgctgcaa accattcttg atgaagtgcg cagtgcgaaa cttgcgggta
      601 tgtgccctgt gcagaccgac caacatgttc tgaatgtcgt gtcgcatcag ctacattgat
      661 catcatcggc gctaatgcat tgcgccgatg aagtttttga gaaaccgctg cctcattttt
      721 ttgaagcagc ggttttttaa tgggattcgc cctgtggggt aaacttgagt tcaatcaacg
      781 cgatggcttt ttggattgcc cgcatggtga ccgggtctgc ggcggcggga tggttagtaa
      841 agtcgatatt ctttagctgg ctggacattt tttcgcgaac ttcaacgggt gcgatcacat
      901 cgagaacatc cagaatttgt ttgataacca actggcaagc aaccacatca gaaaccagtt
      961 cctgatcggc attcagcggc tgggacatcg taaactcctg atagcatttt gaaagccgtt
     1021 atagtagcgg cttcacatct tcagcgatag tcacatccac cgtcatcagg acacaaaaaa
     1081 acctgccgga gcaggttttt tgttatcgga acatattgcc tggcggtacg tctttgaacg
     1141 tcttgcaata gttattgaac atacttttca ggattttgcg cagtttcatc gcggcactcc
     1201 gaccatttgt tatacaggtg ttattgtctt tgcgcttata atgtgacaac catcacaaaa
     1261 atcaatcttt atgtgatgca aatcacataa ataccccttt aatgttataa aaatgataat
     1321 caaaaagcag ctaccccatt tctgacacct acagatggca agaaatagcg cctgccaggc
     1381 gtcttttccg gccattgtcg cagcgctgta gcgcgtaaaa tagtgctttc ttttactctt
     1441 ctggctggac catgagactt ctgattctga ctctttcatt aataacgctg gcggggtgta
     1501 cggttactcg ccaggcccat gtgagcgaag ttgacgcggc aacaggtatt gttcggttgg
     1561 tttatgatca ggcttttttg cagcatgccc gtaccgatcg ttatgtcagt cgcgggatcg
     1621 ccgatcgcgc atgccagcag gcaggctata cccacgctat cccctttggt cagccagtag
     1681 gcaactgtag cctctttgcg ggttccttat gtttgaatac ggaattcact ttgtcatatc
     1741 agtgccatca ttccgccttc cctgtctttc tctaaacgtt ttaatcagcg agggggatct
     1801 tcgctgatta aataaatagc ggtaatgcgt tttattccca ttcgta
//
LOCUS       DAZFLD010000431         1835 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.433, whole genome shotgun sequence.
ACCESSION   DAZFLD010000431 DAZFLD010000000
VERSION     DAZFLD010000431.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1835
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.433"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            121..1125
                     /gene="msrP"
                     /locus_tag="ACHKBL_004997"
     CDS             121..1125
                     /gene="msrP"
                     /locus_tag="ACHKBL_004997"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016667 - oxidoreductase activity, acting
                     on a sulfur group of donors [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HFO5821715.1"
                     /translation="MKKNQFLKESDVTAESVFFMKRRQVLKALGISAAALSLPHAAHA
                     DLLSWFKGNDRPPAPAGKPLEFSKPAAWQNNLPLTPADKVSGYNNFYEFGLDKADPAA
                     NAGSLKTDPWTLKISGEVAKPLTLDHDDLTRRFPLEERIYRMRCVEAWSMVVPWIGFP
                     LHKLLALAEPTSNAKYVAFETIYAPEQMPGQQDRFIGGGLKYPYVEGLRLDEAMHPLT
                     LMTVGVYGKALPPQNGAPVRLIVPWKYGFKGIKSIVSIKLTHERPPTTWNLAAPDEYG
                     FYANVNPHVDHPRWSQATERFIGSGGILDVQRQPTLLFNGYAEQVASLYRGLDLRENF
                     "
     gene            1126..1761
                     /gene="msrQ"
                     /locus_tag="ACHKBL_004998"
     CDS             1126..1761
                     /gene="msrQ"
                     /locus_tag="ACHKBL_004998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HFO5821716.1"
                     /translation="MRLTAKQVTWLKVCLHLAGLLPFLWLVWAINHGGLGADPVKDIQ
                     HFTGRTALKFLLAALLITPLARYAKQPLLIRTRRLLGLWCFAWATLHLTSYALLELGV
                     NNLALLGKELITRPYLTLGIISWVILLALAFTSTQAMQRKLGKHWQQLHNFVYLVAIL
                     APIHYLWSVKIISPQPLIYAGLAVLLLALRYKKLLSLFNRLRKQVHNKLSL"
BASE COUNT          445 a          457 c          464 g          469 t
ORIGIN      
        1 cgtggcagtt aatgcataaa tatataaaag cagatctctg ctactgttag ggaggtgact
       61 ccctgacaga ttaacagtaa acggctcttg ctggctaacg acaaaaaagt gtgatggctt
      121 atgaaaaaga atcaattttt aaaagaatca gatgttacgg ccgagtcggt attctttatg
      181 aagcgtcggc aggtgttaaa agcactgggc atcagcgcgg ctgcactatc tttgcctcac
      241 gctgcgcatg ccgatctgct tagctggttt aaagggaacg atcgtccacc cgcccccgcc
      301 ggaaaaccgc tggagttcag caagcctgcc gcctggcaaa ataacctgcc actgacgcca
      361 gcagataaag tttccggtta taacaacttc tatgaattcg ggctggataa agccgatccc
      421 gccgctaatg ctggtagcct gaaaaccgat ccatggacac tgaaaatcag cggcgaagta
      481 gcaaaaccat taaccctcga tcacgatgat ttaacccgtc gcttcccgct ggaagagcgt
      541 atttatcgta tgcgctgcgt ggaagcatgg tcgatggtgg tgccgtggat tggttttccg
      601 ctgcacaaat tgctggcgct tgccgaaccc accagcaatg cgaagtatgt cgctttcgaa
      661 acaatttatg caccggaaca gatgccaggc cagcaggacc gctttatcgg cggcgggctg
      721 aaatatcctt atgtcgaagg attgcgtctc gacgaagcaa tgcatccgct cacactgatg
      781 accgtaggtg tttatggcaa ggcgttaccg ccacaaaatg gcgcgccggt gcgactgatt
      841 gtgccgtgga aatatggctt taaagggatt aaatcgatcg tcagtattaa gctgacccac
      901 gagcgtccgc caaccacctg gaatctggca gcgcctgacg aatacggttt ttacgccaac
      961 gttaatccgc atgttgatca cccgcgctgg tcacaggcta ccgaacgatt tattggttca
     1021 ggcggcatcc tcgatgtaca gcgccagcca acgctactgt ttaatggtta cgccgagcag
     1081 gtggcatcgc tgtatcgtgg cctggatttg cgggagaatt tctaaatgcg tctgacagca
     1141 aaacaggtga catggctgaa agtttgcctg catcttgccg gattgttgcc gtttctctgg
     1201 ctggtctggg cgattaatca cggtggattg ggtgccgatc cggtgaaaga tattcagcat
     1261 tttaccggtc gcactgcact gaaatttttg cttgcggcct tgttaatcac acctctggca
     1321 cgctacgcaa aacagccgtt attgatacgc actcgccgcc tgttaggctt atggtgcttt
     1381 gcctgggcga cactgcattt aaccagttac gcattactgg agctgggcgt gaataatctg
     1441 gcgttattag gtaaggagtt aattacccgg ccttatttaa cgttaggaat tatcagctgg
     1501 gttattttgc ttgctttagc gtttacttca acccaggcga tgcagcgaaa actcggcaag
     1561 cactggcaac agttgcataa tttcgtctat cttgtcgcga tcctggcccc aatacattat
     1621 ctgtggtcag tgaagattat ttcaccgcag ccactcatct acgctgggct ggcggtactg
     1681 cttttagcct tacggtataa gaagttgctt tcccttttca accggttacg caagcaggtt
     1741 cacaacaaat tatctctata acttgatatt gccgacaaag tacgcgttat ccctgccgga
     1801 tgcggcgcga gcgccttatc cggcatacac agatc
//
LOCUS       DAZFLD010000432         1824 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.434, whole genome shotgun sequence.
ACCESSION   DAZFLD010000432 DAZFLD010000000
VERSION     DAZFLD010000432.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1824)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1824)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1824
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.434"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(205..1488)
                     /gene="gltA"
                     /locus_tag="ACHKBL_004999"
     CDS             complement(205..1488)
                     /gene="gltA"
                     /locus_tag="ACHKBL_004999"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004108 - citrate (Si)-synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HFO5821717.1"
                     /translation="MADTKAKLTLNGDTAVELDVLKGTLGQDVIDIRTLGSKGVFTFD
                     PGFTSTASCESKITFIDGDEGILLHRGFPIDQLATDSNYLEVCYILLNGEKPTQEQYD
                     EFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYHDSLDVNNPRHREI
                     AAFRLLSKMPTMAAMCYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPCEPYEVNPILER
                     AMDRILILHADHEQNASTSTVRTAGSSGANPFACIAAGIASLWGPAHGGANEAALKML
                     EEISSVKHIPEFVRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRETCHEVLKELGTKD
                     DLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAMARTVGW
                     IAHWSEMHSDGMKIARPRQLYTGYEKRDFKSDIKR"
     gene            complement(1694..1801)
                     /locus_tag="ACHKBL_005000"
     CDS             complement(1694..1801)
                     /locus_tag="ACHKBL_005000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5821718.1"
                     /translation="MRKSYEVGISPKINLCNSVEVLTNSFGTVISGRQV"
BASE COUNT          481 a          423 c          436 g          484 t
ORIGIN      
        1 cattgggtta acaatgctac cggcaggaaa gtaaaaaaat aaacttatca ttctgctcag
       61 tatgtgactt gtcacatttt ttcaccttat taatgggtat ttgcatctgg tttaattttt
      121 acaatttatt atgttcagcc atataaaaaa gaacccgctc tattaaaggc gggtccggaa
      181 agtaaacggc ttagcaatca accattaacg cttgatgtcg cttttaaagt cgcgtttttc
      241 atatcctgta tacagttgac gcggacgggc aatcttcata ccgtcactgt gcatttcgct
      301 ccagtgggcg atccagccaa cggtgcgtgc catagcgaag atcacggtga acatagaaga
      361 cggaataccc atcgctttca ggatgatacc agagtagaaa tcgacgttcg gatagagttt
      421 cttctcgata aagtacgggt cgttcagcgc gatgttttcc agctccatag ccacttccag
      481 caggtcatct ttggtaccca gctctttcag cacttcatgg caggtttcac gcattacggt
      541 ggcgcgcggg tcgtaatttt tgtacacgcg gtgaccgaag cccatcaggc ggaaagaatc
      601 atttttatct ttcgcacgac gaacaaattc cggaatgtgt ttaacggagc tgatttcttc
      661 cagcattttc agcgccgctt cgttagcacc accgtgcgca ggtccccaca gtgaagcaat
      721 acctgctgcg atacaggcaa acgggttcgc ccccgaagag ccagcggtac gcacggtgga
      781 ggtggaggcg ttctgttcat ggtcagcgtg caggatcaga atacggtcca tagcacgttc
      841 cagaatcgga ttaacttcat acggttcgca cggcgtagag aacatcatat tcaggaagtt
      901 accggcgtag gagagatcgt tgcgcgggta aacaaatggc tgaccaatgg aatacttgta
      961 acacatcgcg gccatggtcg gcattttcga cagcaggcgg aacgcggcaa tttcacggtg
     1021 acgaggattg ttaacatcca gcgagtcgtg atagaacgcc gccagcgcgc cggtaatacc
     1081 acacatgact gccattggat gcgagtcgcg acggaaagca tggaacagac gggtaatctg
     1141 ctcgtggatc atggtatgac gggtcaccgt agttttaaat tcgtcatact gttcctgagt
     1201 cggtttttca ccattcagca ggatgtaaca aacttccagg tagttagaat cggtcgccag
     1261 ctgatcgatc gggaaaccgc ggtgcagcaa aataccttca tcaccatcaa taaaagtaat
     1321 tttagattcg caggatgcgg ttgaagtgaa gcctgggtca aaggtgaata caccttttga
     1381 accgagagta cggatatcaa taacatcttg acccagcgtg cctttcagca catccagttc
     1441 aacagctgta tccccgttga gggtgagttt tgcttttgta tcagccattt aaggtctcct
     1501 tagcgcctta ttgcgtaaga ctgccggaac ttaaatttgc cttcgcacat caacctggct
     1561 ttacccgttt gttatttggc tcgccgctct gtgaaagagg ggaaaacctg ggtacagagc
     1621 tctgggcgct tgcaggtaaa ggatccattg atgacgaata aatggcgaat caagtactta
     1681 gcaatccgaa ttattaaact tgtctaccac taataactgt cccgaatgaa ttggtcaata
     1741 cttccacact gttacataag ttaatcttag gtgaaatacc gacttcataa cttttacgca
     1801 ttatatgctt ttcctggtaa tgtt
//
LOCUS       DAZFLD010000433         1822 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.435, whole genome shotgun sequence.
ACCESSION   DAZFLD010000433 DAZFLD010000000
VERSION     DAZFLD010000433.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1822
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.435"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..594
                     /locus_tag="ACHKBL_005001"
     CDS             <1..594
                     /locus_tag="ACHKBL_005001"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFO5821719.1"
                     /translation="WWVLGVSNRLAWGCPTKEHLLPHFLEHLGNNHLDIGVGTGFYLT
                     HVPESSLISLMDLNEASLNAASTRAGESKIKHKISHDVFDPYPAALHGQFDSISMFYL
                     LHCLPGNISTKSCVIRNAAQALTDDGTLYGATILGDGVVHNSFGQKLMRIYNQKGIFS
                     NTKDSEEGLTHILSEHFENVKTKVKGTVVMFSASGKK"
     gene            complement(676..1018)
                     /locus_tag="ACHKBL_005002"
                     /pseudo
     CDS             complement(676..1018)
                     /locus_tag="ACHKBL_005002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416064.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            1031..1626
                     /locus_tag="ACHKBL_005003"
                     /pseudo
     CDS             1031..1626
                     /locus_tag="ACHKBL_005003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415069.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
BASE COUNT          512 a          430 c          416 g          464 t
ORIGIN      
        1 tggtgggttt tgggtgtatc aaatcggctt gcatggggat gtcctacaaa ggaacacctt
       61 cttccacact ttctggaaca tttaggtaac aaccatctgg atattggcgt tggaactggg
      121 ttttacctta ctcacgtacc tgagagtagt ctgatatctt taatggattt gaacgaagct
      181 agcctgaacg cggcatcgac aagggctggg gaatcaaaaa ttaaacataa aattagccat
      241 gatgtttttg atccttatcc cgcggcgtta catggtcaat ttgattccat ttccatgttt
      301 taccttcttc actgcctgcc tggaaatata tctacaaaaa gctgtgtaat acgcaatgcg
      361 gcgcaggcct taactgacga tggaactcta tacggagcca caattcttgg tgacggcgtt
      421 gtgcacaata gcttcggtca aaaactgatg cgcatttaca atcagaaagg catcttttca
      481 aacacaaaag attccgaaga aggcttaaca catatactct cagagcattt cgagaatgtt
      541 aaaaccaagg ttaaaggtac tgtagtaatg ttttccgctt cagggaaaaa atagcatcca
      601 gccgcagcac gttcttgcgc acgacgttct gcggcataat cccaatgatt actctctgac
      661 agggttcaca ggccactaaa tatcaggggc agttgatgta tcaactcggt tcagcaacac
      721 ccgatacatc ttccaggctt ccagcaacga ggtttcttcc tccgtatcct ttatccatga
      781 tatgccgttc cacttctgaa actcccctgc cggggatacc tggtgacatt ttccggtaac
      841 ggaccgagtt cagaaataaa taatgcgtcc cctgatgcca cgtcataaac cgttttaccc
      901 cgatggtctt caacgagatg ccacgatgcc tcatcactgt tgaaaacaac cacgaagcca
      961 gccggaatat ctgggcaata tcagtactgt ttgcaggcaa acctgtatga ggtggaatgg
     1021 cacaagagac ttacaaaaga atcaggccaa acgcctgatt gatgagagcc ccgaaccact
     1081 gaaatctgtc gtcaaatttg cgctggtaac tgttctgaga aagtcgaaca tcataaatct
     1141 ggagtggcaa cagattgaca tgcagcgaca agttgccagg gtgaatccag aagatagcaa
     1201 atcaaactgc gccattggtg tggcgctgaa cgataccgcc tctaaagtgt tgcgtgatca
     1261 aacaggcaag catcacaaat gtgggtgtac ataccagggc ggctaagcga gcagatggaa
     1321 catcaatgcc tgcgctcagg aagatgcgca ttgacagcaa gacagcatgg ctatcagctt
     1381 gtcgtcgtgc aggaattgaa aatttccgtt tccatgacct ctggcatacc tgggcaagct
     1441 ggttgattcc gtcaggcgtc ccattatcag tgcttcagga aatgggcgga tgggagtcca
     1501 gagaaatggt tcgtagatat gctcaccttg cgcctaatca tttgactgag cacgcgagga
     1561 aaatagacga catttttggt gataatgtcc cactctggaa ttatagaaga aataaagaag
     1621 gcgtaactga ttgaatttta atggcgcgcc ctgcaggatt cgaacctgcg gcccacgact
     1681 tagaagttcc tagaacgaca ttttaagtca acaacttacc gcgccatctc tgcgctcaca
     1741 cgtcccacta cctcaaaaca tgtaaagcct tgcaagccat tgcgaggcct tatgtgtctc
     1801 agttttgtcc ctcttttttg ta
//
LOCUS       DAZFLD010000434         1817 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.436, whole genome shotgun sequence.
ACCESSION   DAZFLD010000434 DAZFLD010000000
VERSION     DAZFLD010000434.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1817
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.436"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            128..892
                     /locus_tag="ACHKBL_005004"
     CDS             128..892
                     /locus_tag="ACHKBL_005004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpK"
                     /protein_id="HFO5821720.1"
                     /translation="MKKHLFTLTLSSVLAIPAVSHAEFKGGFADIGIHYLDWTSRTTE
                     KSSTKSHKDDFGYLELEGGANFSWGEMYGFFDWENFYNDRHDKPGSEQRYTFKNTNRI
                     YLGDTGFNLYLHAYGTYGSANRVNFHDDMFLYGIGYNFTGSGWWFKPFFAKRYTDQTY
                     YTGDNGYVAGWVAGYSFMLGSEKFTLTNWNEYEFDRDATYAAGNGGKEGLNGAVALWW
                     NATSHITTGIQYRYADDKLGEDFYQDAIIYSIKFNF"
     gene            complement(930..>1817)
                     /locus_tag="ACHKBL_005005"
     CDS             complement(930..>1817)
                     /locus_tag="ACHKBL_005005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO5821721.1"
                     /translation="VAQEKSFSRAGERIGLSQSAVSHSVKELENHTGVRLLDRTTREV
                     VLTDAGQQLALRLERLLDELNSTLRDTGRMGQQLSGKVRVAASQTISAHLIPQCIAES
                     HRRYPDIQFILHDRPQQWVMESIRQGDVDFGIVIDPGPVSDLQCEAILSEPFFLLCHR
                     DSALAAEDYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQANIVQEIGHPATLFP
                     MVAAGIGISILPALALPLPEGSPLVVKRITPVVERQLMLVRRKNRSLSTAAQALWEVV
                     RGQGRALMAGREGDPLYQI"
BASE COUNT          460 a          479 c          421 g          457 t
ORIGIN      
        1 tttcactatt aataggctga aaaaactgta ttggtactct cctttccctt gcattaacaa
       61 tatgccgctt ttaatcaacc ttttattaag caatcaacta ataacgatcg gcatagggaa
      121 ttatgctatg aaaaaacatc tttttactct gacactctct tctgtattgg cgataccagc
      181 agtatcccat gcagaattta aaggcggttt tgctgatata ggtattcatt atcttgactg
      241 gactagcaga accactgaga aatcgtcgac caaatcacac aaagatgatt ttggatatct
      301 ggaactggaa ggcggcgcta actttagttg gggagaaatg tacgggttct tcgactggga
      361 aaacttttat aatgatcgtc atgataaacc gggtagcgag cagcgttata cctttaaaaa
      421 taccaaccgt atttacctgg gtgacaccgg atttaatctc tacttgcatg cgtatggcac
      481 ctacggttct gcgaatcgcg tgaatttcca tgacgatatg ttcctttacg gtatcggcta
      541 caatttcacc ggtagcggtt ggtggttcaa accgttcttt gcaaaacgct atacagacca
      601 aacctattat accggcgata acggttatgt cgccggttgg gttgcaggtt acagctttat
      661 gctgggcagt gagaaattca ccctcaccaa ctggaacgag tacgagtttg atcgtgatgc
      721 cacctacgcg gcaggtaatg gcggcaaaga aggtctgaac ggtgccgtcg cactttggtg
      781 gaatgcaact tcacacatca ccacaggaat tcagtatcgt tatgcggatg acaaactggg
      841 cgaagatttt taccaggatg caataatcta ctccatcaaa tttaatttct gattgattac
      901 tgccggggct cccggcagta tgaatagctc tatatctgat acagcggatc cccttcccgc
      961 ccagccatta gcgcacggcc ctggcctcgt accacttccc ataacgcttg tgcggcggtg
     1021 gaaagtgaac ggttcttcct gcgcaccagc atcaactggc gttccaccac cggcgtaatg
     1081 cgctttacca ccagcgggct accttcaggt aacggcagcg ccagtgcggg aagaatacta
     1141 atgccgatgc ctgcggctac catcgggaac agcgtcgccg gatgaccaat ctcctgcaca
     1201 atattcgcct gaataccgtt gcgcgccagt gctgcatcaa tcagcggtcg gctgcctgac
     1261 gcgtaatcct gcaacaccaa tttcgctccc tgtaatgcct gccagggtac ataatcttcc
     1321 gcggccaaag cgctatcgcg atggcaaagc aagaagaaag gctcggaaag aatcgcttca
     1381 cattgcagat cgctcactgg gcctggatcg atgacgatgc caaaatcgac atctccctgg
     1441 cgaatacttt ccatcaccca ctgctgcgga cgatcgtgca ggataaactg aatatctgga
     1501 tagcggcgat ggctttcagc aatgcattgc ggaataagat gcgcggaaat ggtctggctg
     1561 gcagcgaccc gaacttttcc gctcagttgt tgccccatac gcccggtatc gcgcaacgtg
     1621 ctgttaagtt catccagcag tcgctcaaga cgcaaagcca actgctgccc tgcatctgta
     1681 agcaccactt cacgcgtggt tctgtccagc aggcgaacac cagtatgatt ttccagctcc
     1741 ttcacactgt gactcactgc cgactggctc aggccgatac gctctcctgc acgactaaaa
     1801 cttttctcct gcgctac
//
LOCUS       DAZFLD010000435         1807 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.437, whole genome shotgun sequence.
ACCESSION   DAZFLD010000435 DAZFLD010000000
VERSION     DAZFLD010000435.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1807
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.437"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            17..508
                     /locus_tag="ACHKBL_005006"
     CDS             17..508
                     /locus_tag="ACHKBL_005006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO5821722.1"
                     /translation="MGKKTAPLLPYTNQMLSEFGERLKLARLRRRLTAKQVAERAGMS
                     PITLRSLESGSAGVTIGAYLSVMQVLGLETDLNKLAAEDDLGRQLQDSRLIKKRGLSR
                     ASSKNEEGSQTASLQMRHEKEGHLTTDLTALAEGSTPSITGETSTSLAALLKPIKKPL
                     KGN"
     gene            511..1779
                     /locus_tag="ACHKBL_005007"
     CDS             511..1779
                     /locus_tag="ACHKBL_005007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001606689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HipA family
                     toxin"
                     /protein_id="HFO5821723.1"
                     /translation="MALIGVYADWEGLDGPERIGYLHSRRTRTREIFEFEYDKKALAD
                     PSLNFIQLDPEIMLYEGAQYPIPPKDKFGAFSDSCPDRWGRMLMKRRFERDIRDGLCD
                     KDSHLYESDYLLGVHDLYRVGALRYKREDAGEFLDNRIDVAAPPFTEIASLERASRAI
                     EEDPDNKELMGQKWLRMLIAPGGSLGGARPKASVVDEAGHLYIAKFPSVKDEYDVGGW
                     EMAVNALAVGCGLNVAPAQAHKFASNYHCFMVRRFDRTNAGRRLHFASAMTLTRHQDG
                     EDASTGVSYLELADVLIRHGAQTNTDLKELWSRIVFNILVSNTDDHLRNHGFILIPGK
                     GWRLSEAYDLNPVVRSDGLKLNITENDNALDLELAREVAEYFRLSLTEADDIIANFRG
                     IVSQWRIIAERLRLPGREQELMAEAFRASI"
BASE COUNT          500 a          330 c          470 g          507 t
ORIGIN      
        1 ataaaatatt atcactatgg gtaaaaaaac agcaccgtta ctcccttata cgaatcagat
       61 gcttagtgaa tttggtgagc ggctgaaact tgctcgttta cgacgcaggc ttaccgcaaa
      121 gcaagttgct gaacgtgctg ggatgtcgcc aataacattg cgctctctgg agtcaggaag
      181 tgcaggggtc acgatagggg catatttgtc tgtgatgcag gttttgggcc tggaaacgga
      241 tttaaataag cttgcagctg aagacgactt agggcgtcag ctccaggatt cccgtttgat
      301 taaaaaaaga gggctttctc gtgcatcaag taagaatgaa gaaggttctc agacagcctc
      361 attgcagatg aggcacgaga aagaaggtca tctcaccacg gatttaacag cactggcaga
      421 aggctcgacg ccatctatta caggagaaac atcaacctct cttgcagcac tattaaaacc
      481 gataaaaaaa ccgttaaaag gaaattaatt atggcattaa tcggagtgta tgcggattgg
      541 gaaggcctgg atggtccgga gcggattggt tatttacaca gtcgccgcac tcggacgcgt
      601 gagatatttg agtttgagta cgataaaaag gcgcttgcag atccttcact taatttcatt
      661 cagttagatc ctgaaataat gttgtatgag ggagcacagt acccaattcc tccaaaggat
      721 aaatttggtg catttagtga ttcctgcccg gatcgttggg ggcgtatgct aatgaagcgt
      781 cgttttgaga gggatattcg agacgggtta tgtgataaag actcccacct ttatgagtct
      841 gattatctac taggtgttca tgatctttat cgggttgggg cccttcgata caaacgtgaa
      901 gacgctggag agtttcttga taatcgcatt gatgttgcag ccccaccatt taccgaaata
      961 gcgagtctgg aaagggctag ccgggcaatt gaagaagatc ctgataataa agagctaatg
     1021 gggcaaaaat ggttaagaat gctaatcgct ccggggggat cattaggggg ggcacgtccg
     1081 aaagccagtg ttgttgatga agctgggcat ctctacatcg ctaaatttcc cagtgtaaaa
     1141 gatgagtatg atgttggtgg atgggaaatg gccgttaatg cacttgctgt tggctgtgga
     1201 ctgaatgttg cacctgctca ggcgcataaa tttgccagca attaccattg ttttatggtt
     1261 cgtcgttttg accgcactaa tgcgggaagg cgtctgcatt ttgcttcggc tatgacacta
     1321 accaggcatc aggatggtga agatgcctct acaggtgtga gttatctgga attagcagat
     1381 gttcttatca ggcatggcgc gcagactaat actgatctta aggaactctg gtccaggatt
     1441 gtttttaata ttcttgtttc taataccgat gatcatttac gcaatcatgg atttatcttg
     1501 ataccaggaa aaggttggcg gctttctgag gcatatgatt tgaatcctgt ggtcaggtct
     1561 gatggtttaa aactcaatat tacggaaaat gacaatgcac ttgatttgga attagctaga
     1621 gaagttgcag agtattttcg acttagtttg acggaagctg atgacattat tgctaatttt
     1681 agagggattg tcagtcagtg gcgaatcatt gccgagcgtc tgagattacc tggtcgtgag
     1741 caagagttaa tggcagaggc attcagagcg tcaatttaga atagttattt cgatatattc
     1801 gctaaat
//
LOCUS       DAZFLD010000436         1796 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.438, whole genome shotgun sequence.
ACCESSION   DAZFLD010000436 DAZFLD010000000
VERSION     DAZFLD010000436.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1796
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.438"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(91..852)
                     /gene="kduD"
                     /locus_tag="ACHKBL_005008"
     CDS             complement(91..852)
                     /gene="kduD"
                     /locus_tag="ACHKBL_005008"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HFO5821724.1"
                     /translation="MILSAFSLEGKVAVVTGCDTGLGQGMALGLAQAGCDIVGINIVE
                     PTETIKQVTALGRRFLSLTADLRKIDGIPALLDRAVAEFGHIDILVNNAGLIRREDAL
                     EFSEKDWDDVMNLNIKSVFFMSQAAAKHFIAQGNGGKIINIASMLSFQGGIRVPSYTA
                     SKSGVMGVTRLMANEWAKHNINVNAIAPGYMATNNTQQLRADEQRSAEILDRIPAGRW
                     GLPSDLMGPVVFLASSASDYVNGYTIAVDGGWLAR"
     gene            complement(882..1718)
                     /gene="kduI"
                     /locus_tag="ACHKBL_005009"
     CDS             complement(882..1718)
                     /gene="kduI"
                     /locus_tag="ACHKBL_005009"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008697 -
                     4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HFO5821725.1"
                     /translation="MDVRQSIHSAHAKTLDTQGLRNEFLVEKVFVADEYTMVYSHIDR
                     IIVGGIMPVTKTVSVGGEVGKQLGVSYFLERRELGIINIGGAGTITVDGQCYEIGHRE
                     ALYVGKGAKEVVFASIDTATPAKFYYNCAPAHTTYPTKKVTPDEVSPVTLGDNLTSNR
                     RTINKYFVPDVLETCQLSMGLTELAPGNLWNTMPCHTHERRMEVYFYFNMDDDACVFH
                     MMGQPQETRHIVMHNEQAVISPSWSIHSGVGTKAYTFIWGMVGENQVFDDMDHVAVKD
                     LR"
BASE COUNT          469 a          476 c          405 g          446 t
ORIGIN      
        1 attatttcat aaccatgata tggattatga tgatctacag gtataaaaaa ccctgccatg
       61 cggcagggtc ataaaagtaa gagaaatgaa ttaacgagcc agccaaccgc catccacggc
      121 aatggtataa ccattcacat aatctgaagc gctggaggca aggaacacta ccggccccat
      181 caggtcactc ggcagtcccc aacggccagc tggaatgcgg tcgagaattt ccgcgctacg
      241 ttgttcatct gcccgtagtt gttgagtatt gttggttgcc atgtaacccg gagctatcgc
      301 attaacatta atgttgtgtt tagcccattc attcgccatc aatcgcgtca cgcccatcac
      361 gccgcttttt gatgcggtat aagaaggcac acggatcccg ccctggaagg agagcattga
      421 cgcgatattg ataatcttgc cgccattgcc ttgcgcgata aagtgtttcg ccgctgcctg
      481 agacatgaag aatacgctct tgatattcag gttcatgaca tcgtcccagt ctttttcgct
      541 gaactcgaga gcgtcttcgc ggcgaatcaa tccggcgtta ttcaccagga tatcaatatg
      601 accaaactca gctaccgcgc gatccagcag tgctggaatg ccatcaatct ttcgcagatc
      661 ggcggtcagg cttaaaaaac gacgccccag cgccgtgacc tgcttgatgg tttcagtcgg
      721 ttcaacgatg ttaatgccaa caatgtcaca gcctgcttgc gccagcccca acgccatccc
      781 ctggcccagc ccagtatcac aaccagtgac gaccgcaact ttaccttcga gagaaaatgc
      841 acttaaaatc ataacaatac cttattcgtt tatgcccaca actagcgcaa atctttaacg
      901 gccacatggt ccatatcatc aaaaacctgg ttttcaccga ccatgcccca gataaaggta
      961 taagctttgg ttccgacgcc ggaatggatc gaccagctcg gggagatcac cgcctgctcg
     1021 ttatgcatca caatgtgacg cgtttcttgc ggttgcccca tcatgtggaa aacgcaggcg
     1081 tcatcatcca tattgaaata gaaataaact tccatccggc gctcgtgggt gtgacacggc
     1141 atggtgttcc acaagttacc tggagccagc tccgtcagcc ccatactcaa ttggcaggtt
     1201 tccagcacat ccgggacaaa atatttgtta atcgtgcgac ggttactggt gaggttatcg
     1261 cctaacgtga ctggagatac ttcgtccggt gtgacttttt tggtgggata cgtcgtatgc
     1321 gcgggtgcgc aattgtaata aaacttcgcc ggagtggcgg tatcaatact ggcaaagaca
     1381 acttcttttg cacctttacc aacatacagg gcttcgcggt gaccgatttc atagcattga
     1441 ccatcgacag taatcgtacc ggcaccgcca atattgataa tacctaactc gcgacgttca
     1501 aggaaatagc ttacgccgag ttgtttacca acttccccgc caacggaaac cgttttagtt
     1561 accggcataa tgccgccaac aataatacgg tcaatgtggc tgtaaaccat ggtgtactca
     1621 tcggcgacaa ataccttttc aaccaaaaat tcattgcgca gcccctgggt atccagcgtt
     1681 tttgcgtgcg cactgtggat gctctgtctt acgtccacat caacctccga taatcagtga
     1741 agcgtgaaaa tagaaaacga tagtgaaacg ctgtttcgtt ttctctataa ggggat
//
LOCUS       DAZFLD010000437         1769 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.439, whole genome shotgun sequence.
ACCESSION   DAZFLD010000437 DAZFLD010000000
VERSION     DAZFLD010000437.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1769
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.439"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            43..816
                     /locus_tag="ACHKBL_005010"
                     /pseudo
     CDS             43..816
                     /locus_tag="ACHKBL_005010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416485.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter beta domain-containing
                     protein"
     gene            866..>1769
                     /locus_tag="ACHKBL_005011"
     CDS             866..>1769
                     /locus_tag="ACHKBL_005011"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter adhesin-like protein
                     YeeJ"
                     /protein_id="HFO5821726.1"
                     /translation="MAGPADASQSVLKNNRSSLKGDFTESAELYLVLHDLSGHPINVS
                     EGLEFVQSGTNVSYVQISTIDYTQNLYGEYKATVTGGGEGIATLIPVLNGVHQAGLST
                     TIEFISAGTRPMNGTVSVNGANLPAASFPSQGFTGAYYQLNNDNFAPGKTAADYAFSS
                     TASWVDVDTSGKVTFKNVGDRNAVIITATPRSGGAIYQTQVRVKGWWVNHGNNLMQLS
                     QAENYCSNQVGNGYPLPRADLLSNGHMRREIGSLYGEWGDMGNYMKEADFYSMVYWSS
                     NSAGAGQQYIVSLETGTQNTYQTYE"
BASE COUNT          487 a          413 c          450 g          419 t
ORIGIN      
        1 agaaagggcg catttatgtt agctcgttca gggaaggtaa gcatggctac gaagaagaga
       61 agtggagaag aaataaatga ccaacaaatc ttatgcggga tgggtattaa actacgccgc
      121 ttaactgcgg gtatctgcct ggtaactcaa cttgtgttcc ctatggctgc ggcagcacaa
      181 ggtgtggtaa acgccgcaac ccaacaacca gttcctgcac aaatcgccat tgcaaatgcc
      241 aatacggtgc cctataccct tggagcgctg gaatcggccc aaagcgttgc cgaacgtttc
      301 ggtatttcgt tggctgagtt acgcaaactc aaccagtttc gtacgtttgc tcgaggtttt
      361 gataatgtcc gccagggtga tgaactggat gtcccggcac aagttagtga aaaaaaatta
      421 accccgccgc cgggtaatag cagtgacaac cttgagcaac agatagccag tacttcacag
      481 caaatcgggt ctctgctcgc cgaagatatg aacagcgagc aagcggccaa tatggcgcgt
      541 ggatgggcct cttctcaggc atctggcgca ttgacagact ggttaagccg cttcggtacc
      601 gcaagaatca cgctgggcgt ggatgaagat tttagcctga agaattccca gttcgatttt
      661 ctccatccat ggtatgaaac gcctgataat ctctttttca gccagcatac cctccaccgt
      721 actgacgaac gtacgcagat taacaacggc ttgggttggc gtcatttcac tcccacatgg
      781 atgtcgggca tcaacttctt tttcgaccac gatcttaagc gcagaaacga gctatggtgg
      841 ggcgcaaaca gtagatataa cgctggtggc aggcccggca gacgcctcgc agtccgtcct
      901 taaaaacaac cggtcatcac tgaaagggga ctttaccgaa agtgctgaac tatatcttgt
      961 tttacacgat ctatcaggtc atccgatcaa tgtttctgaa gggctggaat ttgtgcaatc
     1021 aggtaccaac gtgtcctatg tgcaaatcag cacaattgat tacactcaaa atttatacgg
     1081 tgagtataaa gccactgtta caggcggcgg agagggtatc gcaacgctga tccccgtgtt
     1141 gaatggtgtt caccaggcgg gtctgagtac cacgatagaa ttcattagcg cgggaaccag
     1201 accaatgaac ggtacagtat cagtgaatgg tgcaaaccta cctgcagctt cattcccttc
     1261 gcagggattt accggggcgt attatcagtt gaataatgac aactttgcac caggaaaaac
     1321 ggcggctgat tatgcgtttt caagcactgc ctcctgggtt gatgttgata cttccggtaa
     1381 ggtgacattc aaaaatgttg gagatagaaa tgctgtaata atcacggcga ccccaagatc
     1441 aggtggcgct atttaccaaa cgcaagtacg ggtgaagggc tggtgggtga atcacggtaa
     1501 taacctcatg caattaagcc aggctgaaaa ctactgcagc aaccaggtcg gtaatggcta
     1561 cccccttccc cgcgcggacc ttttaagcaa tgggcacatg cggcgcgaaa tagggtcact
     1621 gtatggtgag tggggagata tgggaaatta catgaaggaa gctgattttt attcaatggt
     1681 atattggtct tctaattccg caggcgcagg ccaacaatac attgtatccc tggaaacagg
     1741 gacgcaaaac acttatcaaa cgtatgaat
//
LOCUS       DAZFLD010000438         1734 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.440, whole genome shotgun sequence.
ACCESSION   DAZFLD010000438 DAZFLD010000000
VERSION     DAZFLD010000438.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1734
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.440"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..170
                     /locus_tag="ACHKBL_005012"
     CDS             <1..170
                     /locus_tag="ACHKBL_005012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5821727.1"
                     /translation="LERLFMQTGVSWSDPLRAQYFSVQFSEGIRRAYRHTLATSPYSS
                     YRNPVRRQRFY"
     gene            170..820
                     /locus_tag="ACHKBL_005013"
     CDS             170..820
                     /locus_tag="ACHKBL_005013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6682 family protein"
                     /protein_id="HFO5821728.1"
                     /translation="MTTITEIIGRVNTQLVDPMMVRWPLQELCDYYNDAVRAVILARP
                     DAGASLETISCVPGARQVLPDGVIQLLDVICLSDGSAVRPLSREVLDAQYPEWPTMKG
                     IPECFISNDLSPRVFWLFPVPDKEISIDAVVSRIPEAVYVLTQDDDTPVPLEEAYVNP
                     LVDWMLFRAFSKDAAGGAESGLAAQHYQSFVEQLGIKQGADSALSARKKVFNGGGV"
     gene            817..>1734
                     /locus_tag="ACHKBL_005014"
     CDS             817..>1734
                     /locus_tag="ACHKBL_005014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HFO5821729.1"
                     /translation="MSVVVSGTLKSPDGEAISGANITLTALTVSPDALSGTSASAVTR
                     EGGYYGMTMDPGEYAVSVTVKGKTAVYGRVRIEGTESTVTLNMLLRRSLVEVSIPGEL
                     LTDFRQIQNNVADDLATIRRLNEDTATKNTQATQSKESAAASAKSASDSAKTATSRAA
                     EAGQKASEAAEAATRAITAAGNAEESSTRAGESEKAAGADAEKARLHAEKARLAQESA
                     GEILKRAEAATVSAEEARRMAENARGPRGPQGETGPKGDVGPKGETGPVGPQGPAGPK
                     GERGDVGAQGAVGPAGPRGEKGEQGERGPQ"
BASE COUNT          416 a          391 c          566 g          361 t
ORIGIN      
        1 cacttgagcg gttgttcatg cagactggtg tttcatggtc agatccgtta cgcgcacagt
       61 atttttctgt gcagttttct gaggggatcc gtcgggcata tcgtcataca ctggcgacaa
      121 gcccgtactc ttcataccgc aaccctgtac gcaggcagag gttttactga tgacgacgat
      181 tacagaaatc atcggacgtg tgaatacaca actggttgac ccgatgatgg ttcgctggcc
      241 cctgcaggaa ttgtgcgatt attacaatga tgctgtgagg gcagtgattc tggcgagacc
      301 ggatgctggc gcaagcctgg aaacaatcag ttgtgttcct ggcgcccgtc aggttttgcc
      361 cgatggtgta atacaacttc ttgacgtgat atgcctcagt gatggtagtg cagtcagacc
      421 attatcccgg gaggtgctgg atgcgcagta tcctgagtgg cccacaatga agggcattcc
      481 tgaatgtttt atcagcaacg acctgtcccc gcgcgtattc tggctgtttc ctgtccctga
      541 caaagagata agtattgatg cagtggtaag ccggataccg gaggcagtgt atgttctgac
      601 gcaggacgat gatacgccag ttccactgga agaggcttat gttaacccac tggtggactg
      661 gatgttgttt cgtgctttca gtaaggatgc tgccggtggt gcagaatcgg ggctggctgc
      721 gcagcattat cagagttttg ttgagcaact tgggatcaaa cagggggcag acagtgcatt
      781 gtctgcccgt aaaaaagtgt ttaacggagg tggagtgtga gtgttgttgt ttcggggacg
      841 ctgaaatctc ctgatggtga ggcgatatca ggagcaaata ttaccctgac ggcgctgaca
      901 gtttcaccgg atgcgctcag cggcaccagt gcgtcggcag tgacccgtga aggtggatat
      961 tacggaatga cgatggatcc gggggagtat gcggtttcgg tgacggtgaa agggaagact
     1021 gctgtctacg gacgtgtgcg tattgagggg accgaaagta cggtgacgct caatatgctg
     1081 ttacgccgca gtcttgttga ggtgagcata cccggagagc tgctgacaga tttccggcag
     1141 atacagaata atgtggctga tgaccttgct actattcgtc gcctgaatga agacacggcg
     1201 acaaaaaaca ctcaggccac acagtcaaaa gaaagtgcag cagccagtgc gaagagtgca
     1261 tctgacagtg caaagacggc aaccagcagg gcggctgaag ccggacaaaa agccagtgaa
     1321 gctgcagaag cagcgacccg tgcaatcaca gcagcgggga atgcagagga aagctcgacc
     1381 cgtgccggag agtctgaaaa agccgccgga gctgatgcag aaaaagccag actgcatgct
     1441 gaaaaggcca ggctggcgca ggagagcgcc ggagagatcc ttaagcgggc agaggctgcc
     1501 actgtcagtg ctgaagaggc cagacgtatg gctgagaatg cacgggggcc ccgggggcct
     1561 cagggagaaa ctggtccgaa gggggatgtc ggtcctaaag gcgaaacagg tccagtgggc
     1621 cctcaagggc ccgcagggcc gaaaggtgag cgtggtgacg ttggtgctca gggggcagta
     1681 gggcccgctg gtccgcgggg tgagaagggc gaacaggggg agcgaggacc gcag
//
LOCUS       DAZFLD010000439         1730 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.441, whole genome shotgun sequence.
ACCESSION   DAZFLD010000439 DAZFLD010000000
VERSION     DAZFLD010000439.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1730
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.441"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1587
                     /locus_tag="ACHKBL_005015"
     CDS             <1..1587
                     /locus_tag="ACHKBL_005015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000874423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6645 domain-containing protein"
                     /protein_id="HFO5821730.1"
                     /translation="IKQLARRSTVTPGGAACIYNDIIPADHCLHDVEDMTGLNHPKAD
                     LSKGQYGCVGQGLHIAKKLLPFIPANAGILLVPCCRGGSAFTTGTDGTYSDTTGASES
                     STRWGVGRPLYKDLIGRTKAALAKNPKNVLLAVVWMQGEFDFNGTPANHAARFTEVVD
                     QYRADLADMAGQCVGGSADSVPWICGDTTYFWKQKNETAYQTVYGSYKNKTEKNIHFV
                     PFMTDENGVNVPTNKPEEDPDIPDIGYYGSKWRTDRSTWTSQDRASHFSSWARRGIIS
                     DRLATAILSCAGKSSAFVNGTAGVVVPDRPVTTSESVIFYDAKKASDNQLKPYGWDGM
                     DGRRTLVDDSGNKALRIEKNNSAKSWSMYCDIAADKAKLLLEKGGEIAVRFKIPENVN
                     LETTRNKYAFGLYWRIAEWPGEGGEGYLSSFFVQTDKASIDVAYHHTVNQQKELGTFG
                     AFDHDWHTLAFKFKGSNSINVTPVLDGVDGQAFDLVKWANTANGLNRFVITDITGSAE
                     TYPVLIDTVEVKANKAGAAA"
BASE COUNT          483 a          357 c          475 g          415 t
ORIGIN      
        1 attaaacagt tagcgcgtcg cagtacggtg acaccgggag gtgctgcatg tatctataac
       61 gatattatcc cggcagatca ctgtctccat gatgtggagg atatgacagg gcttaatcat
      121 cccaaagcgg atttatcgaa aggtcagtac ggctgtgtgg gacaggggct gcatatcgcc
      181 aaaaagcttc tgccatttat accggcgaat gcgggcattc ttcttgttcc gtgctgccgt
      241 ggtggttcag ctttcaccac cggaaccgat ggcacataca gtgacactac tggcgcctca
      301 gagagttcca cccgctgggg ggtgggcagg ccgctgtata aggacctcat tggtcgtaca
      361 aaagccgcgc tggcgaagaa tccgaaaaat gtgctgcttg ccgtggtatg gatgcaaggg
      421 gaatttgatt ttaacggaac gccagcgaat catgcagccc gttttacaga agtggtggat
      481 caataccgtg cggaccttgc agatatggcg gggcagtgtg tgggtggctc agctgacagt
      541 gttccctgga tttgtggaga cacaacatat ttctggaagc agaagaacga aacggcgtac
      601 cagacggtgt atggtagtta caaaaataaa acggaaaaga atatccattt cgtgccgttc
      661 atgaccgatg agaacggggt gaatgtgccg acgaacaaac cggaagaaga cccggatatt
      721 ccggatatcg ggtattacgg ttcaaaatgg cgaacggaca gaagcacctg gacatctcag
      781 gacagggcca gtcatttcag ttcatgggct cgccgtggga ttatttccga ccgtctggca
      841 acggcgattc tgagctgcgc gggtaagtct tctgcgtttg ttaatggtac tgccggggtg
      901 gttgttccag acagaccggt taccacctca gagtctgtaa ttttttacga tgccaaaaaa
      961 gcttcagaca atcagctgaa accttatggc tgggacggta tggatggcag acgcacactg
     1021 gttgatgaca gcggcaataa agctctgcga attgagaaaa ataacagcgc gaaatcctgg
     1081 tcaatgtact gtgatattgc tgcagacaag gcaaaacttt tactggaaaa aggcggggaa
     1141 attgctgtcc ggtttaaaat ccccgaaaac gtcaatcttg agacaaccag aaacaagtat
     1201 gcctttggtt tgtactggcg aatagcggaa tggccgggtg agggtggtga aggctatctg
     1261 agttctttct ttgtccagac agataaagcc agtattgatg ttgcatacca tcatacagtt
     1321 aatcaacaaa aagaacttgg cacgtttggc gcattcgacc atgactggca tacgcttgca
     1381 tttaaattta agggcagtaa cagcattaat gttactccgg tgcttgatgg tgtggatgga
     1441 caggcgtttg acctggtgaa atgggccaat actgctaatg gactcaacag gtttgtcatt
     1501 acggatatta caggtagtgc agaaacctac cctgtactta ttgatacggt ggaagttaaa
     1561 gcaaacaaag ctggagcagc cgcataattg ctaaaaaaag ccgccagcgg caggaatgga
     1621 agctggcgga ggtaatccca atggagaatg taaagaaaag atgctttcgt acattggttt
     1681 tttaaatgaa aacagttctc attgtcaacc ataacggtaa gaaattatga
//
LOCUS       DAZFLD010000440         1728 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.442, whole genome shotgun sequence.
ACCESSION   DAZFLD010000440 DAZFLD010000000
VERSION     DAZFLD010000440.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1728
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.442"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(113..556)
                     /locus_tag="ACHKBL_005016"
     CDS             complement(113..556)
                     /locus_tag="ACHKBL_005016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFO5821731.1"
                     /translation="MVISIRRSRHEEGEELVAIWCRSVDATHDFLSAAYRAELEELVR
                     SFLPEAPLWVAVNERDRPVGFMLLSGQHMDALFIDPDVRGCGVGRMLVEHALSMAPEL
                     TTNVNEQNKQAVGFYKKMGFKVTGRSEVDDLGKPYPLLNLAYVGE"
     gene            713..1642
                     /gene="metA"
                     /locus_tag="ACHKBL_005017"
     CDS             713..1642
                     /gene="metA"
                     /locus_tag="ACHKBL_005017"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008899 - homoserine O-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0019281 - L-methionine biosynthetic process
                     from homoserine via O-succinyl-L-homoserine and
                     cystathionine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HFO5821732.1"
                     /translation="MPIRVPDELPAVNFLREENVFVMTTSRASGQEIRPLKVLILNLM
                     PKKIETENQFLRLLSNSPLQVDIQLLRIDSRESRNTPAEHLNNFYCNFEDIQEQNFDG
                     LIVTGAPLGLVEFNDVAYWPQIKQVLEWSKDHVTSTLFVCWAVQAALNILYGIPKQTR
                     TDKLSGVYEHHILHPHALLTRGFDDSFLAPHSRYADFPAALIRDYTDLEILAETEEGD
                     AYLFASKDKRIAFVTGHPEYDAQTLAQEFFRDVEAGLDPDVPYNYFPHNDPQNTPRAS
                     WRSHGNLLFTNWLNYYVYQITPYDLRHMNPTLD"
BASE COUNT          424 a          480 c          386 g          438 t
ORIGIN      
        1 aaaggccatc cgaaaggatg gcctttttgc attggcgcag aaaaaatgcc ggatgcgacg
       61 ctggcgcgtc tgctccagct tacgcaagca cgatagcacg atagggggca gcttattccc
      121 ctacatatgc cagattcagc aatggatacg gtttccccaa atcgtccacc tcagagcgtc
      181 ccgtaacctt aaaacccatc ttcttataga acccaacagc ctgcttattt tgctcattaa
      241 cattggttgt cagttccggt gccatcgaga gcgcatgttc caccagcatc cgacccacgc
      301 cgcagccgcg cacatcagga tcgataaaca gcgcatccat atgctgccca cttaacaaca
      361 taaacccaac cggccgatcc cgctcattaa ccgcgaccca caacggcgct tccggcagga
      421 aggaacgaac tagctcctcc agctcggccc gatacgctgc tgataaaaaa tcgtgagtgg
      481 catcgacaga acgacaccaa atcgcaacga gctcctcccc ttcctcatgc cgtgagcggc
      541 gaatactaat aaccattttc tctccttttc gtcattctta tattctaacg tagccttttc
      601 cttgaaactt tctcaccttc aatatgcagg ctcgacattg gcaaattttc tggttatctt
      661 cagctatctg gatgtctaaa cgtataagcg tatgtagtga ggtaatcagg ttatgccgat
      721 tcgtgtgccg gacgagctac ccgccgtcaa tttcttgcgt gaagaaaacg tctttgtgat
      781 gacaacttct cgtgcgtctg gtcaggaaat tcgtccgcta aaggtgctta tccttaacct
      841 gatgccgaag aagatcgaaa ctgaaaatca gtttctgcgc ctgctttcaa actcaccttt
      901 gcaggtcgat attcagctgt tgcgcatcga ttcccgtgaa tcgcgcaaca cgcccgcaga
      961 gcatctgaat aacttctact gtaactttga agatattcag gaacaaaact ttgatggtct
     1021 gatagttacc ggcgcgccgc tgggcctggt ggagtttaat gatgttgctt actggccgca
     1081 gatcaaacag gtgctggagt ggtcgaaaga tcacgtcacc tcgacgctgt ttgtctgttg
     1141 ggcggtccag gccgcgctca atatcctcta cggcattcct aagcaaaccc gcaccgacaa
     1201 actctctggc gtttacgagc atcatattct ccatcctcat gcgcttctga cgcgtggctt
     1261 tgatgattca ttcctggcac cacattcgcg ctatgctgac tttccggcag cgttgattcg
     1321 tgattatacc gatctggaaa ttctggcgga gacagaagaa ggggatgcct atctgtttgc
     1381 cagcaaagat aagcgaattg cctttgtgac gggccatccc gaatatgatg cgcaaacgct
     1441 ggcgcaggaa tttttccgcg atgtggaagc cggactagac ccggatgtac cgtataacta
     1501 tttcccgcat aatgatccgc aaaatacacc gcgagcgagc tggcgtagtc acggtaattt
     1561 gctgtttacc aactggctca actattacgt ctaccagatc acgccatacg atctacggca
     1621 catgaaccca acgctggatt aatcttctgt gatagtcgat cgttaagcga ttcagcacct
     1681 tacctcaggc accttcgggt gcctttttta tttccgaaac gcacctca
//
LOCUS       DAZFLD010000441         1719 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.443, whole genome shotgun sequence.
ACCESSION   DAZFLD010000441 DAZFLD010000000
VERSION     DAZFLD010000441.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1719
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.443"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..474
                     /locus_tag="ACHKBL_005018"
     CDS             <1..474
                     /locus_tag="ACHKBL_005018"
                     /inference="COORDINATES: protein motif:HMM:NF026977.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5821733.1"
                     /translation="PLGLLGGEQTYRYVPNPCGYVDPLGLAITSIINKTVEFSRGPQT
                     YVSDLGHVTGSTARARNKAITAILKEDFAYLKLTYIPQYNPFMRTGIAKLWEGTQFGK
                     NSFSSRAALRDVIIHEELHHRWWKRGLNDHHPKGTEMEQKFYETIRRYKRMRGWL"
     gene            474..887
                     /locus_tag="ACHKBL_005019"
     CDS             474..887
                     /locus_tag="ACHKBL_005019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5821734.1"
                     /translation="MFGDEFKTTIECPCCGKICSIAIGQTSHNGILAWYESIICHNCG
                     LRSESDDIGFPPEKIRLQLIDFEGLWNVRLENIKSQMETIKVIRKAFSIDTKEALRII
                     RNESKVIYSGTREEAIWLMSLLKHVGETPLIQIAR"
     gene            942..1103
                     /locus_tag="ACHKBL_005020"
                     /pseudo
     CDS             942..1103
                     /locus_tag="ACHKBL_005020"
                     /inference="COORDINATES: protein motif:HMM:TIGR01643.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat domain-containing protein"
     gene            1134..>1719
                     /locus_tag="ACHKBL_005021"
     CDS             1134..>1719
                     /locus_tag="ACHKBL_005021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion protein Rhs"
                     /protein_id="HFO5821735.1"
                     /translation="MNVTTQGRRAIEYHYPDEHTVIRCILPPEDERDRHPGESLLKTT
                     YRYNAAGELTEVILPGDETLTFSRDEAGREVLRHSNRGFACEQGWNAASQLVTQRAGF
                     FPEETTWGGLLPSLVREYRYDSAGNVSAVTSREDYGRETRREYRLDRNGQVTAVTASG
                     TGLGYGEGDESYGYDSCGYLKAQSAGRHRISEETD"
BASE COUNT          478 a          374 c          474 g          393 t
ORIGIN      
        1 ccgctggggt tgctgggggg tgagcagact taccggtatg tgcctaatcc gtgtgggtat
       61 gttgatccgc tgggattagc tataactagt ataattaata aaacagtcga gttttctaga
      121 gggccacaga cttatgtttc tgatctcggt catgttacag gttctacggc cagagccaga
      181 aataaggcaa ttacagccat acttaaagaa gattttgcat atcttaaact aacatatatc
      241 cctcaataca atccgtttat gcgaacaggg attgctaaac tttgggaagg aactcagttt
      301 ggtaaaaata gtttttcgtc tcgtgcagca ttaagagatg ttattattca tgaagaatta
      361 catcataggt ggtggaagcg aggattgaat gatcatcatc ccaaaggtac tgagatggaa
      421 cagaaatttt acgagacgat tcgaagatat aagcggatga gagggtggtt atagtgtttg
      481 gtgatgaatt taaaacgact attgagtgtc cttgctgtgg aaaaatatgt tctatagcta
      541 tagggcagac gagtcacaat ggtatactcg catggtatga atcaattata tgccataatt
      601 gcggattacg tagtgaatca gatgatatag gctttccccc agaaaaaatt cgtctacaat
      661 tgattgattt tgagggttta tggaatgtta gacttgaaaa tataaaatca cagatggaaa
      721 ctataaaagt cattcgtaag gctttttcga ttgatacaaa agaagcactt cgaattatta
      781 gaaatgaatc gaaggtaata tactccggaa cgagagaaga ggccatctgg ttaatgagtt
      841 tattaaaaca tgtgggtgaa acccccttaa tacaaattgc tagataaatt ctatgagctg
      901 ggtacaacgg agtagcctgg cgttaccgct atgacgccga tggcctgtgt accgccaggg
      961 ttaacggtct ggaggaaacc atcctctaca gccgggacgc cgcaggccgc ctggcagaag
     1021 tcatcacgcc ggaaggcaaa acgcagtatg cgtatgacaa atccggcagg ctgacgggta
     1081 tcttcagccc ggacggcaca tcacagcgca ccggctatga cgaacgcggg cgggtgaatg
     1141 tcaccactca gggccgacgg gccattgaat accactaccc cgatgaacac accgtcatcc
     1201 gctgtatcct gccaccggaa gatgaacgcg acagacatcc cggtgaatcc ctgctgaaaa
     1261 ccacctaccg ctacaacgcc gccggagaac tgacggaggt tatcctgccg ggggatgaga
     1321 cgctgacgtt cagccgtgat gaggcaggac gtgaagtgct ccggcacagt aaccggggtt
     1381 ttgcctgtga gcagggctgg aatgcagcca gccagcttgt cacccagcgc gccggatttt
     1441 tcccggagga aaccacatgg ggcgggctgc tcccctcact ggtacgggag taccgttacg
     1501 acagcgcggg caatgtgtcg gctgtcacca gccgggaaga ttacggacgg gaaacacggc
     1561 gggaataccg gctggaccgg aacggtcagg tcacggcggt gacagcctca ggcaccgggc
     1621 tgggctatgg cgaaggcgat gagtcctatg gctatgacag ttgtggctac ctgaaggcgc
     1681 agtctgcggg caggcaccgg ataagtgaag agactgacc
//
LOCUS       DAZFLD010000442         1677 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.444, whole genome shotgun sequence.
ACCESSION   DAZFLD010000442 DAZFLD010000000
VERSION     DAZFLD010000442.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1677
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.444"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..929
                     /locus_tag="ACHKBL_005022"
     CDS             <1..929
                     /locus_tag="ACHKBL_005022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HFO5821736.1"
                     /translation="LHAHDVNITPKPASKARGVIWFAKTRANDDVLIPEGTIVQTARI
                     NGVIYSMAVTEETLISSGKSGASVPVEATTEGSGHNLAPGYFRILPVAIPGIASVVNR
                     EDWLTVPGADEESDDELRDRIRNQFNLVSSYHTDAVYRGLIASVAGLSIDRIYFQHDA
                     PRGPGTANAYLLLDSGEASAPFIEAVNRYINEEGHHGHGDDLQCFAMPETRHRLEVTV
                     YIRSSTTLPPDGAEGLKQDISNLIRCAFRENARFNVKKVWPYSRFSFSTLGKEIHQTF
                     PVVASVQFSLEDIISDLAVPRLESLTVEVVIE"
     gene            922..1470
                     /locus_tag="ACHKBL_005023"
     CDS             922..1470
                     /locus_tag="ACHKBL_005023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO5821737.1"
                     /translation="MNDFLQRLRSLKLPSWMDRGEPDKLLRAAQAFWSRVWEWITWPV
                     RQFDPMTCAEPVLDLLAWERDITRFKGEPTSLYRRRIAHAFVNAQDAGEVAGFIAIFE
                     RLGIGHVELQERKPGVDWDVILVQVTDSQIADNSDLLLEIIRKYGRTCRRYRFEVETL
                     NPFIIHYGAYEGEYICWTARAE"
     gene            1493..>1677
                     /locus_tag="ACHKBL_005024"
     CDS             1493..>1677
                     /locus_tag="ACHKBL_005024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO5821738.1"
                     /translation="MAKSVITSAFIRLKAQQAAEGTPVVLDEFIFANVPDLNPETPVS
                     PDETLPPEDQIVHRQAVG"
BASE COUNT          378 a          483 c          481 g          335 t
ORIGIN      
        1 tgcttcatgc gcacgacgtc aacatcaccc caaaaccggc cagtaaggcc cgtggcgtga
       61 tctggtttgc caaaaccagg gcgaatgatg atgttctcat ccccgaaggg accatcgttc
      121 agacagccag aatcaacggc gtgatataca gcatggccgt cacggaggaa acgcttatca
      181 gcagcggtaa aagcggcgca tccgttcccg tggaagccac aaccgaaggc agcgggcaca
      241 acctcgcgcc ggggtatttt cgtatccttc ccgttgccat tcccggcatt gcatccgtgg
      301 tgaaccggga ggactggctg accgtaccgg gtgccgatga ggaaagcgac gacgaactgc
      361 gcgacagaat acgcaaccag tttaacctcg tgagcagtta ccacacggac gccgtctacc
      421 ggggacttat tgccagcgtg gcgggcctga gcatcgaccg gatctacttt cagcacgacg
      481 ccccgagagg accgggaacg gccaacgcct acctgttact ggacagcggc gaggcctctg
      541 cccctttcat tgaagcagtc aaccgctaca tcaacgaaga ggggcaccac ggacacgggg
      601 atgatttgca gtgtttcgcc atgccggaga cgcgtcaccg tctggaggtc accgtataca
      661 tcagaagttc aaccaccctg ccaccggacg gcgcggaagg gctgaaacag gatatcagca
      721 acctgatacg ctgcgctttc cgggagaacg cccgctttaa tgtgaaaaaa gtctggccct
      781 attcgcgctt ttcgttttcc acgctgggca aggaaattca tcagaccttc ccggtggtgg
      841 cttcggttca gttttcactg gaagacatca tcagcgatct ggccgtgcca cggctcgaat
      901 ccctgacagt ggaggtggtc attgaatgac tttttgcagc ggcttcgctc gctgaagctg
      961 ccctcctgga tggacagggg agaacccgac aaactgctgc gggccgcgca ggcgttctgg
     1021 tcccgcgtat gggaatggat cacctggccc gtgcgccagt ttgaccccat gacatgtgca
     1081 gagcccgtac ttgatttact ggcatgggag cgggacatca cccgttttaa aggtgaaccc
     1141 acatcactgt accgccgccg tatcgcccac gcctttgtca atgcacagga cgccggagag
     1201 gtggcgggct ttatcgccat ttttgaacgg ctgggcattg gacatgtgga gttgcaggag
     1261 cgcaagcccg gcgttgactg ggacgtgatc ctggtgcagg tgacagacag ccagatagcc
     1321 gacaacagcg atcttcttct ggagatcatc cgcaaatatg gcaggacatg ccgccgttac
     1381 cgttttgagg tggaaaccct taaccccttc atcatccatt atggcgcata cgaaggggag
     1441 tatatctgct ggacggccag ggcagagtga atgactgaag gagtatgact ttatggcgaa
     1501 atctgtaatt accagcgcct ttatccggct caaggcacag caggcggcgg aaggcacccc
     1561 cgtcgtgctg gatgagttta tttttgcaaa cgtcccggac ctcaacccgg aaacgccggt
     1621 atcccccgac gaaaccctgc cacctgaaga tcagattgtt caccgccagg ccgttgg
//
LOCUS       DAZFLD010000443         1675 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.445, whole genome shotgun sequence.
ACCESSION   DAZFLD010000443 DAZFLD010000000
VERSION     DAZFLD010000443.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1675
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.445"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            3..>1675
                     /locus_tag="ACHKBL_005025"
     CDS             3..>1675
                     /locus_tag="ACHKBL_005025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001532370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HFO5821739.1"
                     /translation="MLNSSISLILASGLMMSTANAADDVKLKATKTNVAFADFTPTEY
                     STKGKPNIIVLTMDDLGYGQLPFDDSSFDPKTMDNRNVVDTYKIGIDKAIEAAKKSTP
                     TLAALMDEGVKFTNGYVAHGVSGPSRAAILTARAPARFGVYSNSDAQDGIPLEETFLS
                     ELLQNHGYYTAAIGKWHLSKISNVGVPEEKQTRDYHDNFTTFSAEEWQPQNRGFNYFM
                     GYHASGVSLYNSPSLFRNRENIKAEGYISDQLTDEAIGVIDRASKLEQPFMIYLAYSA
                     PHLPNDQPAPKNYQEKFNTGSKTADNYYASIYSVDQGVKKILNTLKEKGQYDNTIILF
                     TSDNGAVIDGPLPLNGAQAGYKGQPYPGGMHTPMFMWWNGKLKTGDYDKLVSSMDFYP
                     TALDAADIKIPASLKLDGVSLLPYVQKGADKKSAAGEPHKYLSWITSYSHWWDEENTA
                     FWNGYHKFVRKESDDYPHNPHTEDLSQFAYTVRSNDYSLVYTYAENKLALYDINDLQQ
                     TKDLSASHPDVVKNMKAVMIDFITSSQKPISDINQAKFENIKKSLTNVK"
BASE COUNT          531 a          368 c          338 g          438 t
ORIGIN      
        1 aactgcttaa ctcatcaata tcacttatat tagcatctgg tctgatgatg agtactgcaa
       61 atgcagcaga tgatgttaag ctcaaagcaa caaaaaccaa tgttgccttt gcggacttca
      121 cgccgacgga atacagcact aaaggtaagc caaatatcat tgttttaaca atggatgatt
      181 tagggtatgg acaattgcct tttgatgata gttcatttga ccctaaaacg atggataacc
      241 gaaatgttgt tgatacatac aaaataggta ttgataaagc aattgaagct gcaaaaaaat
      301 ccactccaac attagcggcc ctgatggacg aaggtgtaaa attcactaac ggctatgttg
      361 cacacggtgt ctctggccct tcacgtgccg caatcctgac ggcgcgagct cctgctcgct
      421 tcggtgtata ttcaaattct gatgcacagg atggtatccc tctggaagaa acattcctgt
      481 ctgaattact tcagaatcat ggttattaca ctgcagcaat cggtaagtgg cacttatcaa
      541 aaatcagcaa cgtaggtgtg cctgaagaaa aacaaacccg tgactaccac gataacttca
      601 ccaccttctc cgcagaagaa tggcaacctc agaatcgtgg cttcaactac ttcatgggtt
      661 atcacgcatc cggcgtttca ctttataact ccccgtcatt gttccgtaac cgtgaaaaca
      721 ttaaagctga agggtacatt agtgatcaat taactgatga agcaattggt gtaattgacc
      781 gtgcaagcaa actggaacag ccatttatga tttatctggc atacagtgca ccacacctgc
      841 ctaacgatca gcctgcgcct aaaaactatc aggagaaatt caacactggt agcaagactg
      901 ctgataacta ttatgcgtca atttactccg ttgaccaggg ggttaaaaag atcctgaata
      961 cacttaagga aaaaggtcag tacgacaaca ccatcattct gttcacctct gataacggtg
     1021 ctgtaattga tggtccactg ccactgaatg gtgcgcaggc tggttacaaa ggccaaccgt
     1081 atccgggtgg tatgcataca cctatgttca tgtggtggaa tggcaagctg aaaacgggtg
     1141 attatgacaa actggtttca tcaatggact tctatccgac cgctttagat gccgcagata
     1201 tcaagatccc tgcttctctg aaactagatg gtgtatcatt attgccatat gtacagaaag
     1261 gagctgataa gaaatctgca gctggcgaac ctcacaaata tctgtcctgg attacctctt
     1321 attctcactg gtgggacgaa gagaacactg cattctggaa cggatatcat aaattcgtga
     1381 gaaaagagtc tgatgattat ccgcacaacc cacatacaga agacttaagc cagtttgcat
     1441 acactgtaag aagcaacgat tattctctgg tttacaccta tgcagagaat aaactagctc
     1501 tgtacgatat caacgatctg caacaaacta aagatctctc tgcatcccat cctgatgtag
     1561 ttaagaatat gaaggctgtt atgattgact tcatcacgtc aagtcaaaaa ccgatcagtg
     1621 atatcaacca agccaaattt gagaatataa aaaaatcttt aactaacgtg aaatg
//
LOCUS       DAZFLD010000444         1642 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.446, whole genome shotgun sequence.
ACCESSION   DAZFLD010000444 DAZFLD010000000
VERSION     DAZFLD010000444.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1642
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.446"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..546
                     /locus_tag="ACHKBL_005026"
     CDS             <1..546
                     /locus_tag="ACHKBL_005026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2345 domain-containing protein"
                     /protein_id="HFO5821740.1"
                     /translation="IRFVTDKIRELQSAVLLGSAPQGVALTSGEHLQLCSTRNTMINA
                     GQHLDIGAMKNLSVTVEKALGLFVHKDGAKLVANQGDIEIQAQHNTMALFSEKQLTVT
                     SSEDEIIISTPETLTLNGGGSYLRLSKNGIEHGSSGEFIVKTSDYLVPGSGVDLPNEA
                     PGFSLTDITQESKISSKSFND"
     gene            562..>1642
                     /locus_tag="ACHKBL_005027"
     CDS             562..>1642
                     /locus_tag="ACHKBL_005027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5821741.1"
                     /translation="MSVLPKIVWPIPSSRRGSEFKNQEEILSHLGGESTGLYMIGRSG
                     MWHGGIHITEATTPWCALSGKSPLEAIDFPVPFKGEQAIRCMADGEVIAYRICRDYLT
                     IAWESGPLNFSGSFVLVKHYIQPGEKETSGLHFYTLYMHLAPYSAYESEAENQWVVQD
                     TLKAYSEMDWLTAKLTSDETSPQIAGYMPEGARVEWNPADSNLNATGSNKRKYGLVTL
                     KGLPEKNSSTLTPGQRYWVVVDNNNVKPAPGAGPGWWRHLLPPAKEAMVFDKTVSLSS
                     PFAIKVGDPIGHMGYYQAPKDGGYEARYQVHIECTSMDDNLEKFLTNPEQVGKNNPLW
                     LKYAPGLALYKKDAFTGTFTKDARVT"
BASE COUNT          443 a          371 c          455 g          373 t
ORIGIN      
        1 attaggtttg tgaccgacaa aatcagggaa cttcagtctg cggtcctgct gggtagtgcg
       61 ccgcaggggg tggccctgac ctcaggtgaa caccttcagc tctgcagtac ccgtaacacc
      121 atgatcaatg caggccagca tcttgatatc ggtgcgatga aaaatctgtc ggtaacggtg
      181 gaaaaagccc ttggtctgtt tgtgcataaa gacggggcga agctggtagc caaccaggga
      241 gatattgaga ttcaggctca gcacaacacg atggcgctgt tctctgaaaa acagctgacg
      301 gtgaccagta gtgaagacga aattattatc agcaccccgg aaactctgac gctgaacggt
      361 ggtgggtctt acctgagact gagtaagaac gggatagagc acggctcatc gggtgagttt
      421 attgtgaaga cgtctgacta tctggtgcct ggttctggcg tagatctccc caacgaagca
      481 cctggtttca gcctgacgga tatcacacag gaaagcaaaa tcagcagcaa gtcttttaac
      541 gactgacagg gagtatgaat cgtgtctgta ttacccaaaa tcgtctggcc gatcccctcc
      601 agccgtcggg gaagtgagtt caaaaatcag gaagagatcc tctctcattt ggggggagaa
      661 tctaccgggc tgtacatgat aggtcgtagt ggcatgtggc atggtgggat ccatatcacc
      721 gaagccacga caccatggtg tgcgcttagc ggtaagtctc cacttgaagc gatagatttt
      781 ccggttcctt ttaaaggtga gcaggcaatc cgatgcatgg ctgacggaga agtgattgct
      841 taccgcatct gcagggatta tctgacgatt gcatgggaat ccggtccgct gaatttttcc
      901 ggctcatttg ttctggtgaa acactatatc cagccgggtg agaaagaaac cagtggactg
      961 catttctata ctctgtatat gcatcttgcg ccgtattcag cttatgaatc tgaagcagaa
     1021 aatcagtggg tcgttcagga cactctaaaa gcatattctg aaatggactg gctgacggcg
     1081 aaactgactt cagatgaaac ttctccacaa attgcagggt acatgcctga aggggccaga
     1141 gtggagtgga acccggcaga cagcaatctt aacgctacag gaagtaacaa gagaaaatac
     1201 ggtcttgtga cgctaaaggg tttaccagaa aaaaacagca gtactctcac tcccggtcag
     1261 cgctactggg ttgttgtgga taacaacaat gttaagccag cgccgggggc cgggcccggc
     1321 tggtggcgac acttgctgcc tcccgcaaag gaagccatgg tattcgataa aacagtaagc
     1381 ctcagttctc catttgccat taaggtcggt gacccgattg ggcacatggg ctactatcag
     1441 gccccaaagg atggggggta tgaggcacga tatcaggtac atatcgaatg cacgagtatg
     1501 gatgataatc tggagaaatt cctgaccaat cctgagcagg tgggaaaaaa taatccactc
     1561 tggctgaagt acgccccggg gcttgcgttg tacaagaaag atgcttttac aggaacattc
     1621 acgaaggacg cgagagtgac ga
//
LOCUS       DAZFLD010000445         1624 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.447, whole genome shotgun sequence.
ACCESSION   DAZFLD010000445 DAZFLD010000000
VERSION     DAZFLD010000445.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1624
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.447"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..789
                     /locus_tag="ACHKBL_005028"
     CDS             <1..789
                     /locus_tag="ACHKBL_005028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000376547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO5821742.1"
                     /translation="LLSCIAFACLYVCFSGVTANAALITVMALCCAAVFISFIYQGMG
                     KRWPTGIMLMLVVMFCFLYGWPLQAFLPTWLKVDMQYSPETVALIFMLAGFGSAAGSC
                     IGGFMGDWLGTRKAYVISLLIGQLVIIPVFLVDRDYVWLLGLLIFTQQVFGQGIGALV
                     PKIISGYFNVEQRAAGLGFIYNVGSLGGACAPILGAVVASHTSLGTAMCSLAFILTFV
                     VLVLIGFDMPSRVQRWIHPEAALEYDTVDGKPFYGARKKNVAGE"
     gene            793..1623
                     /locus_tag="ACHKBL_005029"
     CDS             793..1623
                     /locus_tag="ACHKBL_005029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000948499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFO5821743.1"
                     /translation="MLSGNKKMIHYRENIYSLDNYERYITDNSLFENTDDGIIYTGIV
                     PLITVVEPVVSNQHSVLIIPGGGYRRIVYEREGLDVGRWIAGQGYTAFILSYRMPSVN
                     GYYSFADAKNALNFISYWAGMSERTFKSVGCMGFSAGGHLAGYITHLHYEEETSVRPD
                     WMALIYPVISLDRSIAHQGCRKRLLDAGESEYSASLENSVTEYMPPVFIVHATDDKDV
                     PVIHSLRYFNSIRDKGHHAEAHFFDKGGHGFGVSGKNGYPIEKWQSLLLEWLAEHKFH
                     "
BASE COUNT          403 a          268 c          406 g          547 t
ORIGIN      
        1 cttctgtctt gtattgcatt tgcatgtcta tatgtttgtt tttcaggcgt cactgcaaac
       61 gctgctttaa tcacagttat ggcattgtgc tgtgcggctg tttttatcag ttttatatat
      121 cagggtatgg ggaaaagatg gccaacaggc attatgttga tgcttgtggt tatgttctgt
      181 ttcctctacg gatggccact gcaggctttt cttcctacat ggcttaaggt tgatatgcag
      241 tattcaccag aaactgttgc tctcatattt atgctggcag gttttggctc tgcagctgga
      301 agttgtatag gtggttttat gggggactgg cttggaaccc gaaaagcata tgttatatca
      361 cttcttatcg gtcagcttgt tattatcccc gtttttcttg tggaccggga ctatgtatgg
      421 ttacttggcc ttctgatctt tactcagcag gtttttggtc aggggattgg tgctctggtg
      481 ccgaaaataa tcagtggtta ttttaatgtg gagcagcgtg cggcaggact ggggtttatt
      541 tataatgtag gttcacttgg tggagcatgt gcgcctattc ttggtgcagt tgttgcgtct
      601 catacaagtc ttggtacagc aatgtgttct cttgctttta tcttaacatt tgttgttctt
      661 gtccttattg gttttgatat gccatcacgt gtacagagat ggatccatcc agaagctgcg
      721 cttgaatatg atactgtcga tggaaaacct ttttatggag caagaaaaaa gaatgttgca
      781 ggagaataaa ggatgctatc agggaataaa aaaatgattc attacaggga gaatatatat
      841 tctcttgata attatgagcg ttacataact gacaattcgc tatttgagaa tactgacgat
      901 ggcataatat atacaggtat tgtgcccctt attacagttg ttgagcctgt tgtatctaat
      961 caacatagtg tgctgattat acctggaggt ggatacagga ggattgttta tgaaagagag
     1021 ggacttgatg taggcagatg gatagcaggt cagggatata cggcatttat tctttcttac
     1081 agaatgccct ccgttaatgg ttattattcc tttgcggatg cgaagaatgc cctgaacttt
     1141 atttcttact gggccggaat gtcggagaga acatttaaga gtgttggatg tatggggttt
     1201 tcggcaggtg gtcatcttgc tggatatatt actcatctcc attatgagga agagacttct
     1261 gtgcgtcctg actggatggc tctgatatat ccagttatat ctcttgacag gagtattgct
     1321 catcagggat gccggaaacg tcttctggat gcaggtgaaa gtgaatattc agccagtctt
     1381 gaaaatagtg ttacagagta tatgccacca gtctttattg tgcatgcaac agatgataag
     1441 gatgttccag ttattcactc tctgagatat tttaactcta tccgggataa gggacatcat
     1501 gcagaagctc atttttttga taagggaggg catggatttg gtgtatccgg aaaaaatgga
     1561 tatccgatag aaaaatggca aagcctgctt ctggaatggc ttgcggaaca taagtttcat
     1621 tgag
//
LOCUS       DAZFLD010000446         1623 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.448, whole genome shotgun sequence.
ACCESSION   DAZFLD010000446 DAZFLD010000000
VERSION     DAZFLD010000446.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1623
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.448"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            104..346
                     /locus_tag="ACHKBL_005030"
     CDS             104..346
                     /locus_tag="ACHKBL_005030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5821744.1"
                     /translation="MMMARDITAYHLNSVHISARRFPGIFQWATGMQTKNLPLTNSLL
                     QNDYAESIFVFNGERDVSEMPLPEEMLLTGNAESER"
     gene            complement(1272..>1623)
                     /locus_tag="ACHKBL_005031"
     CDS             complement(1272..>1623)
                     /locus_tag="ACHKBL_005031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HFO5821745.1"
                     /translation="SLTLKQATAYSDISTRRALSEANRYTDNKFRNLSNKIERAEKRL
                     NAGIAGVTAISSIPYVAENSFSYGIGVGNYQNANAVAAGIQYKTSRNTNIRLNISWDS
                     SHNTAIGAGVAGGW"
BASE COUNT          401 a          330 c          395 g          497 t
ORIGIN      
        1 cagcagtaac tggctatcgt gtataattat ccagaagtcc tgctgagcct tccgtctgtt
       61 catcatgtgc caggtttgcg ttcgggatat gatgtgccat cttatgatga tggcaagaga
      121 tattactgcg taccacctca attctgtaca catcagtgcc cgtcgtttcc ccgggatttt
      181 tcagtgggcc accggtatgc aaacaaaaaa tctgccgtta actaattctt tgttacagaa
      241 tgattacgca gaaagtattt ttgtatttaa tggcgaacgt gacgtttcgg aaatgccttt
      301 accggaagaa atgctactga ctggaaacgc tgagtcagaa agataattcg gtgtattcat
      361 gactgttatc aatgttttat gtgtatgatg tcaacgacgg atgaaaagtt atccacttat
      421 atgaggcatt ataggattat ttctggctga cgttgtttca gttcagacca ggggcagtga
      481 atatgctgac ctgctttttc gctgatatca gtccccctgt aattacatag ataatagata
      541 tacctctccg ttgatcaggc tgtccaggta attgataccg cacttcctca tcagttcgta
      601 gcagagtgta cttagcatgg ctagtgcagt ttctaatgat ttacagtgtg tattcaggag
      661 agtaagaaat tgctcaaaac atcctggttt atactggtgc tttgatattc attgtgtccc
      721 ttttccgttc gttattattt ccatatttaa tatgatgcct gtacatgtaa aataatggaa
      781 gatttcaggg gagcattgca taaagtccgc ctggagaata aggctcaggt cagcgaatcg
      841 catcgtggcc agactgcact gattgcaata agtgcggata cagagttgca gctactcgtc
      901 gatatcagaa caacagaatc cttgcaaaca gggatgtgtt cattcagggg gtatgatgct
      961 acgacgaacg cttttagtga ttattaatcg acgatactgt gttttgtctg gatggtgagg
     1021 gactcgcttt gaggattcac cagggaaata cggggcttgt ggcaataatg ttagcctgcg
     1081 aacttaaatg tttacgggca acatggcggc aggaaatctg ccgccggatt gttgtgaagg
     1141 ctgaccacac tgcgcgccct ttttccttga ttatcccgct cagtccagtg gtctgttccg
     1201 gatggggctg tgttgcgtac tgaattaatg tgattacgga ataccataat gccgtatgta
     1261 cgcaagatta tttaccagcc gccggcaacc cctgcaccaa tcgcggtgtt atgagatgag
     1321 tcccaggaga tgttgaggcg tatattggta ttacgtgatg ttttatactg aatgcctgcg
     1381 gccacagcat tagcgttctg atagttaccc acgccgatcc cgtatgaaaa agaattttca
     1441 gccacatagg ggatggagga tattgctgtc actccggcga tgcctgcatt aagtcttttt
     1501 tctgcccgtt caattttatt gctcaggttt ctgaatttgt tgtcggtata acgattggcc
     1561 tctgacagtg cccggcgggt actgatatcg ctgtaggctg ttgcctgttt cagtgtcagt
     1621 gaa
//
LOCUS       DAZFLD010000447         1607 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.449, whole genome shotgun sequence.
ACCESSION   DAZFLD010000447 DAZFLD010000000
VERSION     DAZFLD010000447.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1607
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.449"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            84..341
                     /locus_tag="ACHKBL_005032"
     CDS             84..341
                     /locus_tag="ACHKBL_005032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5821746.1"
                     /translation="MDEERVFSLSYEQLTRLAEKRIRECNLDSQGAIYLCESAKAGAV
                     LIFWHELAINGYASMNAIKRQELIDADFQRLRNLIWPEDDR"
     gene            341..931
                     /locus_tag="ACHKBL_005033"
     CDS             341..931
                     /locus_tag="ACHKBL_005033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5821747.1"
                     /translation="MTQSRQPSPLQRRVLIVLAALDEKRPGPVLTRDIERVLERSGEA
                     PVYGPNLRASCRRLEDAGWLRTLRAPNLQLAVELTDAGRAVAQPLLLAEQDRLRAEQR
                     AAEVVVLPLIPATGLPADGTSATDLAVELNGITYQACRGDFVVRLDGSTCLQLWNKEG
                     RVVRREGDPLEVAQWLQACHDAGMEVRVQVNESITP"
     gene            complement(949..1296)
                     /locus_tag="ACHKBL_005034"
     CDS             complement(949..1296)
                     /locus_tag="ACHKBL_005034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6404 family protein"
                     /protein_id="HFO5821748.1"
                     /translation="MTFEQKKARAIALMDSKKMWRSNYAPPLLRILWRLGIRLPPLPF
                     MPFWQVTLLMGSLWGISWGCAMWFIYWGPSGMVAGEAIIISITGGFLFGLLTASFHWW
                     RRKVNRLPPWDDV"
     gene            1415..>1607
                     /locus_tag="ACHKBL_005035"
     CDS             1415..>1607
                     /locus_tag="ACHKBL_005035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5821749.1"
                     /translation="MKLDISVKYFLNIMIADFIIPAPEKISTTWHSDTKMNRFACPIH
                     STNSDYYSGFYHTDVLIPGE"
BASE COUNT          421 a          420 c          424 g          342 t
ORIGIN      
        1 gatattatca gaaatcaata acacaaaaat catatagaat ttgattttta cctgtcagaa
       61 cattatcaga tggagcgcgc aggatggatg aagaaagggt gttttcactc agctatgaac
      121 aactgacccg gcttgcggaa aaacgcatcc gggaatgcaa tctggacagc cagggcgcga
      181 tttatctctg cgagtcagcc aaagccggag ccgtgctgat cttctggcat gaactggcca
      241 taaatggcta tgcctcaatg aatgccataa agagacagga gctcattgac gccgactttc
      301 agcgcctcag aaacctcatc tggccggagg atgacaggta atgacccaga gccgccaacc
      361 gtcgccactg cagcggagag tgctgattgt gctggctgcc cttgatgaaa aacggccagg
      421 ccccgtgctg acgcgggaca tagaacgggt gctggagcga agcggggaag ccccggtcta
      481 cgggccaaac ctgcgcgcct cctgccgtcg tctggaggat gccggctggc tgcgtaccct
      541 gcgcgctccg aacctgcagc tggcagtgga gctgacggac gccggccggg ccgtggcaca
      601 gcctctgctg ctggcagaac aggaccgcct ccgggcagaa cagcgtgcgg cagaagtcgt
      661 cgtactgccg ctgatcccgg caacaggtct gcccgcagac ggcacttctg ccactgatct
      721 ggcggtggag ctgaacggca taacgtatca ggcctgccgt ggggattttg tggtgcgtct
      781 cgacggcagc acctgtctgc agttatggaa taaagaaggc agagtggtac gccgggaggg
      841 cgatccgctg gaagtggcgc agtggctgca ggcctgtcac gatgcaggaa tggaggtccg
      901 tgtgcaggtt aatgaaagca tcaccccgta aaaacatgcc tgcacggttt acacatcatc
      961 ccatggtggc agccggttaa ctttcctgcg ccaccagtgg aaagaggccg tgagcagtcc
     1021 gaacaaaaaa ccacccgtga tacttataat aatggcctca cctgccacca ttcctgacgg
     1081 cccccagtag ataaaccaca ttgcacatcc ccaggaaatg ccccataagc tccccatcaa
     1141 cagcgtgacc tgccagaacg gcataaaggg taatggcgga agccggatac ccagccgcca
     1201 gagaatacgc agaagaggag gggcataatt gctccgccac atctttttgc tatccatcag
     1261 ggcaatggcc cgggcttttt tctgctcaaa ggtcacagga gcacctccgt tccatgatgt
     1321 aaacgtgaca cagcttattg ttattgatta ttattatcat gctgtgatta tgttgtcatg
     1381 caccggtaca cgataacaat aaaggagaaa ccagatgaaa ctggatatat ctgtaaagta
     1441 ttttcttaat attatgatag cagatttcat cattcccgca ccagaaaaaa tatcaaccac
     1501 atggcactca gatactaaaa tgaatcgctt tgcttgccct atacattcta ctaatagtga
     1561 ttactattca ggtttttatc atacagatgt attaattcct ggagaac
//
LOCUS       DAZFLD010000448         1594 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.450, whole genome shotgun sequence.
ACCESSION   DAZFLD010000448 DAZFLD010000000
VERSION     DAZFLD010000448.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1594
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.450"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>1594
                     /locus_tag="ACHKBL_005036"
     CDS             <1..>1594
                     /locus_tag="ACHKBL_005036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HFO5821750.1"
                     /translation="LLIQLERNGHWVTEKDVTINGKTTSQYLTSVILNNLPERPFNIR
                     MVRETADSTTDQLQNRTLWSSYTEIIDVKQCYPNTAIVGLQVDAEQFGGQQLTVNYHI
                     RGRIIQVPSNYDPEKRTYSGIWDGSLKPAYSNNPAWCLWDMLTHPRYGMGKRLGAADV
                     DKWALYAIGQYCDQTVPDGFGGTEPRMTFNAYLSQQRKVWDVLGDFCSAMRCMPVWNG
                     QTLTFVQDRPSDVVWPYTNSDVVVDDNGVGFRYSFSALKDRHTAVEVNYTDPQNGWQT
                     STELVEDPDAILRYGRNLLKMDAFGCTSRGQAHRAGLWVIKTELLETQTVDFTLGSQG
                     LRHTPGDIIEICDNDYAGTLTGGRILSIDAASRTLTLDREVILPETGTSTVNLINGSG
                     KPVRVDITAHPAPDRIQVSVLPDGVETYGVWGLSLPSLRRRLFRCVSIRENTDGTFAI
                     TAVQHVPEKEAIVDNGARFEPLSGSLNSVIPPAVQHLTVEVSASDGQYLALAKWDTPR
                     VVKGVRFSLRLTSGSGENSRLVT"
BASE COUNT          337 a          449 c          520 g          288 t
ORIGIN      
        1 ctgctgattc agcttgagcg taacgggcac tgggtgacgg agaaggatgt caccattaac
       61 ggcaagacca cctcgcagta cctgacgtcg gtgattctga ataatctccc tgagcgcccc
      121 tttaacatcc ggatggtcag ggagacggcg gacagcacca cggaccagct gcagaacaga
      181 acgctgtggt cgtcatacac cgaaatcatc gatgtgaaac agtgctaccc gaacacggct
      241 attgtggggc tgcaggtgga tgcggagcag tttggtggcc agcagctgac ggtgaactac
      301 catatccgtg gtcgcatcat tcaggtgccg tcaaactatg acccggaaaa acgcacctac
      361 agcggtatct gggacggcag tctgaaaccg gcatacagca ataacccggc ctggtgcctg
      421 tgggacatgc tgacccaccc gcgctacggg atgggaaaac gcctgggggc cgcggatgtg
      481 gacaagtggg cactgtatgc catcgggcag tactgtgacc agacggtccc ggatggtttc
      541 gggggaacag agccgcggat gaccttcaat gcgtacctgt cacagcagcg taaggtctgg
      601 gatgttctgg gggatttctg ctcggcgatg cgctgtatgc cggtatggaa cggccagacg
      661 ctgacgttcg ttcaggaccg tccgtcggat gtggtgtggc cgtacaccaa cagcgatgtg
      721 gtggtggatg ataacggcgt ggggttccgc tacagcttca gtgccctgaa ggaccggcac
      781 acggcggtgg aggtgaatta caccgacccg cagaacggct ggcagacttc cacggaactg
      841 gtggaagacc cggacgccat cctgcgctac gggcgcaatc tgctgaagat ggatgcgttt
      901 ggctgtacca gccgcggtca ggcccaccgt gccggactgt gggtgataaa gacagaactg
      961 ctggaaacgc agacggtgga tttcacgctc gggtcacagg ggctgcggca cacgcccggt
     1021 gacatcattg aaatctgtga taacgactac gccgggaccc tgaccggcgg acgcatcctg
     1081 tccatcgatg ccgccagccg taccctgacg ctggaccgtg aggtgatact gccggagaca
     1141 gggacatcga cggtgaacct gattaacggc agcggtaagc cggtgcgcgt ggacatcacc
     1201 gcacaccccg ccccggaccg gatacaggtc agcgtcctgc cggatggcgt ggagacatac
     1261 ggtgtgtggg gactctccct gccgtcactg cgtcgtcgcc tgttccgctg tgtttccatc
     1321 cgggaaaaca cggacggcac ctttgccatc acggcagtgc agcacgtacc ggaaaaagaa
     1381 gccatcgtgg ataacggggc ccgctttgag ccgctgtccg gttcactgaa cagcgtcatc
     1441 ccgccggcag tgcagcacct cacggtggag gtgagtgcct cagacggcca gtatctggcg
     1501 ctggcgaaat gggacacgcc gcgggtggtg aagggcgtgc gcttcagtct gcgcctgacc
     1561 agtggcagtg gtgaaaacag ccgcctggtg acca
//
LOCUS       DAZFLD010000449         1590 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.451, whole genome shotgun sequence.
ACCESSION   DAZFLD010000449 DAZFLD010000000
VERSION     DAZFLD010000449.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1590
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.451"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            130..441
                     /locus_tag="ACHKBL_005037"
     CDS             130..441
                     /locus_tag="ACHKBL_005037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000157082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobC"
                     /protein_id="HFO5821751.1"
                     /translation="MAGKKFYSDEDVQVARGALADLPDLTTQRKTLRDFLEAIKDDIV
                     LLVRTKGYTVTDIRNTLKVAGYDISEKSLRDIIRSATPEKKNRRVARKIGTMSAQKTA
                     E"
     gene            complement(498..827)
                     /locus_tag="ACHKBL_005038"
     CDS             complement(498..827)
                     /locus_tag="ACHKBL_005038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000866645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5821752.1"
                     /translation="MSVLNFRNGTVSVGDVFVSSWGDEQTNVDFYQVVSLHGKTMVSV
                     RKIRAEICGTECRLQSRKPVLSSFCGEPVKRKVFDYSDVPVIHIHECEHAWKRKPDEE
                     HIFTTWG"
BASE COUNT          419 a          422 c          394 g          355 t
ORIGIN      
        1 ggtgcattat agcctgtaat cttggtgcat catagtgcta tagtgacggc agcagcaggg
       61 ctcagccctg cccggagaag cctttgaatt gtggtattta tctgttgctg ttcaggagga
      121 ctaaaaaata tggccggaaa aaaattttac tccgatgaag atgttcaggt tgcacgtggt
      181 gcgcttgcag acctgccaga tttgactacc cagagaaaaa cactccgcga ctttctggaa
      241 gcgataaagg acgatatcgt tctgcttgtc agaacgaaag gttacaccgt aactgacatc
      301 agaaataccc tgaaggttgc tggctatgac atcagcgaaa aatcactacg ggatatcatc
      361 cgcagcgcaa cgcctgagaa aaaaaaccgc cgtgttgcca gaaaaatcgg gaccatgtca
      421 gcacagaaaa cggcagaata agagcgaaaa aaaaggggca taaaagcccc ctgatattat
      481 cctgaccagc acaggaatta tccccatgtg gtgaagatat gctcttcatc cggttttctt
      541 ttccatgcat gttcacactc atgaatatga atcaccggca catccgaata atcaaacact
      601 ttccgtttca ccggctcacc acagaatgat gacaggaccg gcttcctgct ctgtaaccgg
      661 cactccgtgc cacagatttc cgcacggatt tttctcacgc tgaccatagt ctttccatgc
      721 agggaaacaa cctggtaaaa gtcaacgttc gtctgttcat ccccccatga ggatacaaag
      781 acatcaccaa ccgacaccgt tccgtttctg aaatttaata cgctcattgc tgacctccgc
      841 atcacgggtt acctgatatt ctcttcagag gagcaaagct ccttctgccg tccggctctt
      901 caggggatat ttccccgcgg tggcctttcc ggcgggggcc cgtttaccag ctcgcgggac
      961 agctgtccgg gggcagcccg ctaaagtttt tgcctgcttt ccggcaaaaa gttttgcggg
     1021 atgtgcattt cacccctgga ctgatggaac gggagatggt tacgtctgcc ccccggaaag
     1081 tcctctgcgg ggaagtggct gcagaggtgg cggctggagg aaagacagcc gcgccataag
     1141 gcgcggtatc cttacatccc ggcgtctttt ggccgggatg ctcagagcgc cggccacggc
     1201 gcgataaata ccctctgcct taactttcat gccgcaggaa taacgacaca aacacggcag
     1261 ttacagaaaa catatccggg ccagccggat tcaatatccg gttggcccgg ccggcgctga
     1321 atgcccataa aatggcccgg gtataaatac cagggctttt gtttaatcac acacgtggcg
     1381 gcaatgcccc cggataatgt cgcaccagga gcccacatcc ggcgcgacag gagaaagcca
     1441 gcggcacaac aatggcatgt ggcggtcagt ggtgcacagc actcccgcac cggtgaagac
     1501 agagaaaaac cgcacgatat ttacagataa aaaatgaaat tatttcactt tttcacacgg
     1561 tatttatcgc gctgattcgg gaattatgat
//
LOCUS       DAZFLD010000450         1587 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.452, whole genome shotgun sequence.
ACCESSION   DAZFLD010000450 DAZFLD010000000
VERSION     DAZFLD010000450.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1587
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.452"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            36..596
                     /gene="traP"
                     /locus_tag="ACHKBL_005039"
     CDS             36..596
                     /gene="traP"
                     /locus_tag="ACHKBL_005039"
                     /inference="COORDINATES: protein motif:HMM:NF033885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraP"
                     /protein_id="HFO5821753.1"
                     /translation="MRTPSEPPADAGFAVTPPAAESWAEPMPMTETGSGNTMSAPPVA
                     PQSDMQLLARDLKAELDRRDEEIRRTLTMLKESITTLGDTIRKDEAWAQETRQLLDET
                     RKQVHALQMQGPLQIPTGNNGQSTKTPPSRLQGMKIMSVEKGMAWIRWQGSAWAVREG
                     DRLGNITITRIEPSSRSVITSAGTLR"
     gene            593..1123
                     /gene="traQ"
                     /locus_tag="ACHKBL_005040"
     CDS             593..1123
                     /gene="traQ"
                     /locus_tag="ACHKBL_005040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000987018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraQ"
                     /protein_id="HFO5821754.1"
                     /translation="MMSIDALTAIENFASGIFSAGMEFLFTWGELLGIIGLIGHLMRA
                     RAEGRHSMGPGKFIAGIVICGMLVALPSFINAGGTQMGFRADSFGPIAYVQPTTFGAA
                     AGAANAMLSLVKLAGVGFAMNGISIWRKSLLDGHTALSSSESISKGNVKFVAGVLLVF
                     IDRVIDASLASLGLAF"
     gene            1170..1568
                     /locus_tag="ACHKBL_005041"
     CDS             1170..1568
                     /locus_tag="ACHKBL_005041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001605438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6750 family protein"
                     /protein_id="HFO5821755.1"
                     /translation="MSRFRAMAARLCAAFIYVQLCLVACRDRLIRLLLLLPVILLPHT
                     AMANTLFDMIKTVGDGAKGSSSSFVAIAQVIGLVLFIAGLLGLKQIGKKPGAGLACLL
                     SILVGLCLAVVPEMMGSGQKTLGLSPVSIS"
BASE COUNT          344 a          451 c          438 g          354 t
ORIGIN      
        1 tcctcatcat cctggccgca gtctggtaca agtttctgcg gacaccctca gagccccctg
       61 cagatgcagg cttcgcggtc acaccgcccg ctgctgaaag ctgggcagag cccatgccca
      121 tgacggagac cgggagcgga aataccatgt ccgcgcctcc tgtcgcccca cagtcagaca
      181 tgcagctgct tgcccgtgac ctgaaagcag agctggacag acgggatgag gaaatccgac
      241 gtaccctgac catgctgaaa gagagcatca caacccttgg cgacaccata cggaaagatg
      301 aggcctgggc acaggaaacc cgtcagttgc ttgatgaaac ccgtaagcag gtgcatgccc
      361 tgcagatgca gggaccactg caaatcccga cagggaataa cggccagtca acgaaaaccc
      421 cgccttcccg cctgcagggc atgaaaatca tgtcggtgga aaaaggaatg gcctggatcc
      481 gctggcaggg tagcgcctgg gcagtacggg aaggtgacag gctggggaat atcaccatca
      541 cccggattga accgtccagc cgctcagtca tcacctctgc cggtacatta cgatgatgag
      601 cattgatgcc ctgacagcca tagagaactt tgccagcggt atattcagtg caggcatgga
      661 gtttctcttc acctggggag agctgctggg gattatcggc ctgattggtc atctgatgcg
      721 cgcccgggca gaagggcgtc acagtatggg accgggaaaa tttatcgccg gtatcgtcat
      781 ctgcggcatg ctggtcgccc tgccatcctt catcaatgcc gggggtaccc agatgggatt
      841 cagggcagat tccttcggcc ctatcgctta tgtacaacca accacctttg gtgccgctgc
      901 cggtgccgcc aatgccatgc tctcactggt aaaactggcc ggagtgggat ttgccatgaa
      961 cggcatttcc atctggcgta aatccctgct ggacgggcat accgccctga gcagcagtga
     1021 gtccatcagc aaaggaaacg tcaagtttgt tgccggcgtt ctgctggttt ttatcgatcg
     1081 cgtcatcgat gcctctctgg cgagtcttgg tctcgccttc tgatccacat tcattttcag
     1141 taattcagtc tgttagttaa ggagactata tgtcacgatt ccgtgccatg gcggcgcgtc
     1201 tgtgcgctgc atttatttat gttcagttat gcctggtggc ctgccgtgac cggctgatac
     1261 gcctgttact tctgcttccc gtgatactgc tgccccacac agcgatggca aacaccctat
     1321 tcgatatgat caaaaccgtt ggcgatggtg caaaaggctc cagcagttca tttgtggcca
     1381 tcgctcaggt tattggcctg gtgctcttta ttgccggcct gcttggcctc aaacaaatcg
     1441 gtaaaaaacc cggagcaggg ctggcctgcc tgctgtcaat tctggtcggc ctctgccttg
     1501 ccgtcgtacc ggaaatgatg ggcagcggac agaaaacgct cggtctttct ccggtttcaa
     1561 tcagttaatc cgcaaaggca ccgggaa
//
LOCUS       DAZFLD010000451         1585 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.454, whole genome shotgun sequence.
ACCESSION   DAZFLD010000451 DAZFLD010000000
VERSION     DAZFLD010000451.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1585
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.454"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..614)
                     /gene="tssL"
                     /locus_tag="ACHKBL_005042"
     CDS             complement(<1..614)
                     /gene="tssL"
                     /locus_tag="ACHKBL_005042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssL, short
                     form"
                     /protein_id="HFO5821756.1"
                     /translation="MRQDIDIDQLMMETWLTVTMLKKGALTPDGTALYNKCVKQVESV
                     REALERAGYDDASVEHITYAQCALLDETVMSRKPEKSGEGGEPDVDEGQAAWRKVPLQ
                     ARFFGSLRAGEALWDRIAEVLRQPSPDPAVLTCFHRVTALGFQGVYGLKAVSQSQRDE
                     VMKALSERVSLPDGGLSLVVYRMEKHRWRLIRSVWFWTVLAVILT"
     gene            complement(611..>1585)
                     /gene="tssK"
                     /locus_tag="ACHKBL_005043"
     CDS             complement(611..>1585)
                     /gene="tssK"
                     /locus_tag="ACHKBL_005043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020884777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
                     /protein_id="HFO5821757.1"
                     /translation="VRYREEWVTLQDAFGSEEEPIAVARFNFTIRFAHENNDSWKVCP
                     VARLIRDGQNAWRQDPSFIPPVALFGASPVLRERLVLLNRQLRSRRQRLMAMRRESNE
                     RLADFAVADVSLFWLLNALNSHASVLTEYERFPSRPPEQVWAELARLAGSMLTFSLDH
                     DPDAIPGYDHKDPAGAFLPLFDLITGLLEACLPSRVIALEMSRPDGQTWKASLHDIRL
                     REEADLWLSVRSDTPAWQVAEKFPALCLAGSPDNVSEIYGAALRGIPLIPVSRVPAAL
                     PVRMENQYFALDMESPAAHEMLEQGVCMFYVPELLGALELELFAVLRS"
BASE COUNT          399 a          474 c          401 g          311 t
ORIGIN      
        1 gtaagaatga cggcgagcac tgtccagaac cagactgagc gtatcagacg ccagcgatgt
       61 ttctccatcc ggtaaacaac cagggacaga ccaccatcag gcagagaaac acgttcagaa
      121 agggctttca tgacctcatc acgctgtgac tggctgactg ctttcaggcc gtacaccccc
      181 tgaaaaccca gtgctgtcac ccggtgaaag caggttaaaa cggccgggtc cggtgaaggc
      241 tgacgcagaa cctctgcaat gcgatcccac agtgcttcac cagcccgaag agaaccaaaa
      301 aagcgggcct gaagcggcac tttacgccag gccgcctggc cttcgtcaac gtccggctcc
      361 ccgccttccc cgcttttttc aggcttacgg ctcatcaccg tttcgtccag gagggcacat
      421 tgtgcatagg taatatgctc cacactggca tcgtcataac cggcacgctc cagcgcttcc
      481 cgcacacttt cgacctgttt aacgcatttg ttgtaaagcg cagtgccatc gggcgtgagt
      541 gcaccttttt tcagcatggt gacggtaagc catgtctcca tcatcagctg gtcgatatca
      601 atatcctgtc tcatgagcgc aataccgcaa acagttcaag ttccagggca ccaagtaact
      661 caggcacata gaacatacac actccctgct ccagcatttc atgtgcagcg ggactttcca
      721 tatcaagtgc gaagtactgg ttttccatac gtacaggcaa cgcagccgga accctgctca
      781 ccgggatcaa agggatcccc ctgagtgccg cgccatagat ctcactgaca ttatcaggtg
      841 aacccgccag acagagtgcc ggaaatttct cagcaacctg ccaggccgga gtatccgagc
      901 gcacagacag ccagaggtcc gcctcttcgc gcaggcggat atcgtgaagg ctggccttcc
      961 aggtctgtcc atccgggcgg gacatttcca gagcaatgac ccgggatggc agacaggcct
     1021 ccagcagacc cgttatcagg tcgaacagcg gaagaaacgc accggccgga tccttgtggt
     1081 cataacccgg tatggcgtca ggatcatgat ccagggaaaa agtcagcata ctgcctgcca
     1141 gacgagcgag ctccgcccac acctgttcag gtggacggga aggaaaacgt tcgtattccg
     1201 tcagtacact ggcgtgggag ttcagcgcat tcagcaacca gaaaagggaa acatcagcga
     1261 cagcaaaatc ggcgagtctt tcgttacttt cacgacgcat cgccatcaga cgctggcgtc
     1321 gggagcgtaa ctgacgattg agcaacacca gacgttcgcg cagaaccgga cttgccccaa
     1381 ataacgccac cggaggaata aaagacggat cctgtcgcca cgcattctga ccatctctga
     1441 tcaatcttgc taccggacag actttccatg aatcattgtt ttcatgggca aaccggatgg
     1501 tgaaattaaa ccgggcaacg gctatgggct cttcttcaga gccaaaagca tcctgcagcg
     1561 tgacccactc ttcccgatag cgaac
//
LOCUS       DAZFLD010000452         1579 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.455, whole genome shotgun sequence.
ACCESSION   DAZFLD010000452 DAZFLD010000000
VERSION     DAZFLD010000452.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1579)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1579)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1579
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.455"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(44..1468)
                     /locus_tag="ACHKBL_005044"
     CDS             complement(44..1468)
                     /locus_tag="ACHKBL_005044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HFO5821758.1"
                     /translation="MFQRSKYDSAYVYALCCVAALAGLMFGYSTAVITGVILPLQQYY
                     QLTSTETGWAVSSIVIGCIIGALVGGKIADKLGRKPALLIIAVIFIVSSLWGAMSESF
                     MVFSISRIVSGFAVGMAGTASTMYMSELAPADIRGKALGIYNISVVSGQVIVFIVNYV
                     IAKGMPSDVLVEEGWKSMLFAQVIPSVTMLVITLFLPESPAWCARNNKGEKRSLKVLA
                     RIYRGVSATDVAAIFDSMRETEARENAAGREFVILKSSPVLRYILLVGCCIAVLQQFT
                     GVNVMNYYAPLVLQNSSTEEVMFQTIFIAVCNVIGSFIGMILFDRYGRIPIMKIGTIG
                     SIIGLLIASYGLYTHDTGYITIFGILFFMLLFAVSWSVGAWVLISEVFPEKIKGFGMG
                     LAVSLMWIANFLISLLFPVINDNTWLQETFGGAFSMWIFVVFNLVCYVFISRYVPETK
                     GIPLTQIEQLAGNKLREIQGKAMM"
BASE COUNT          536 a          423 c          259 g          361 t
ORIGIN      
        1 aacattaccg ggcattactg ctgattgtga cacaatcaga ctattacatc atagctttac
       61 cctgaatttc acgcagttta tttccggcaa gctgctcaat ttgtgttagc gggatcccct
      121 ttgtttcagg aacatagcga gaaataaaaa cataacaaac cagattaaag acaacaaaaa
      181 tccacataga aaacgctccg ccaaaggttt cctgcagcca ggtattatca tttattaccg
      241 gaaacaacag tgatattaga aaattcgcaa tccacattaa actaaccgcc agccccatgc
      301 cgaatccttt aatcttttcg ggaaacacct cagaaatcag tacccatgct cctacgctcc
      361 agctgacagc aaacaacagc ataaaaaata aaatcccaaa aatggtgata tagccagtat
      421 catgggtata caaaccatat gatgcgatca acaaaccaat aattgacccg atagtgccaa
      481 tcttcatgat tggtattcgt ccatagcgat cgaacaaaat catcccgata aaactaccga
      541 tcacattaca aacagcaata aaaatcgtct ggaacatcac ttcctctgta ctgctgtttt
      601 gtagcaccag aggtgcgtag tagttcatca cattgacacc agtaaactgt tgtaaaacag
      661 cgatacaaca gccaaccagc agaatatagc gcaacacagg agagcttttc aggataacga
      721 attccctgcc tgcagcattc tcccgggcct ccgtttccct catactatca aaaatagcgg
      781 ctacatcggt agcgctaacc ccccggtaga ttctggcaag cactttcaga gaacgttttt
      841 cccccttgtt attacgggca caccatgccg gcgattcagg caaaaataaa gttatcacta
      901 acattgtaac agaaggtatc acctgagcga ataacatgct cttccagcct tcttcgacca
      961 gcacatcaga gggcatcccc ttagcaatca cataattaac aataaaaaca atcacctgac
     1021 cagaaactac tgatatattg taaataccca gagctttacc acgaatgtca gctggtgcca
     1081 gttcagacat atacatggta gatgcagtcc ccgccatccc aacagcaaaa ccactgacga
     1141 tgcgcgaaat cgagaaaacc ataaaagatt cactcatcgc cccccacagt gacgagacga
     1201 taaaaataac tgcaataatc agaagcgcag gcttacgccc caacttatcg gcaattttcc
     1261 ccccgaccag cgcaccaatg atacaaccaa tcacaatgct cgatactgcc cacccagttt
     1321 cagttgaagt gagctggtaa tattgctgta aaggcagtat cacccccgtt atcactgccg
     1381 tcgaatagcc aaacatcaat ccagccagtg cagcaacgca gcacaatgcg tacacatagg
     1441 ctgaatcata cttgcttctc tgaaacatac cccctccctg cctgttgagg aaatgacata
     1501 cgaacattta ttccatactt aaacaaatca gctcaaatat tccacaacaa agatcacaaa
     1561 atataaaaat ttcataact
//
LOCUS       DAZFLD010000453         1578 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.456, whole genome shotgun sequence.
ACCESSION   DAZFLD010000453 DAZFLD010000000
VERSION     DAZFLD010000453.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1578
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.456"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(45..686)
                     /locus_tag="ACHKBL_005045"
     CDS             complement(45..686)
                     /locus_tag="ACHKBL_005045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000376467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5821759.1"
                     /translation="MDSAKEIARDMLEAVNINGDGFLGAVGKGLISLPVSLGYMGYDF
                     IDTRHRRENQNDKFRMARLVEKGIFSRDIIGKIISICLEDFVSRINMEKTGSIVKNVS
                     GDLAGRMAFAELTGVKLGEVLVSRGLAAFFAGSFAGLLLSVGAETSRAIYTSRFLKDR
                     NPVLHDKLKAQGDLDLLYFLVEDIVRPFERACIVADRNSAEFDRICEYFFGGL"
     gene            complement(673..>1578)
                     /locus_tag="ACHKBL_005046"
     CDS             complement(673..>1578)
                     /locus_tag="ACHKBL_005046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000431134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFO5821760.1"
                     /translation="SIAQRLRAANTGLTSGNGPQGVFLLVGPSGVGKTETALALADVM
                     YGGEKSLITINLSEYQEPHTVSQLKGSPPGYVGYGQGGILTEAVRKRPYSVVLLDEVE
                     KAHRDVLNLFYQMFDRGFMRDGEGREIDFRNTVILMTSNLGSDLLMQQLSEKPETTES
                     ELHELIRPLLRDHFQPALLARFQTVIYRPLTQSAMRIIVEMKLAQVCERLHCHYGLST
                     SVDERVYDALTSACLLPDTGARNVESLLNQQLLPVLSRQLLSYLAARQHPTYLSLTWN
                     DEDGIVIAFDGINSDYPQESIYGQC"
BASE COUNT          406 a          458 c          343 g          371 t
ORIGIN      
        1 acggataaat cgggaaagca ttataagtaa tgttcttttt atcttcataa accaccgaaa
       61 aaatattcgc atatccggtc aaactcagcc gaatttcggt ctgcgacaat acaggctcgt
      121 tcaaacgggc gcacaatatc ttcaacaaga aagtacaaaa gatccaaatc cccctgagct
      181 ttcagcttat catgcaacac cggatttctg tctttcagaa aacgggatgt gtaaatcgca
      241 cgcgatgtct ctgcgccaac agataataac aacccggcaa aactccctgc aaaaaaagca
      301 gccagccctc tggataccag aacctctccc agtttaaccc cggtcaattc agcaaatgcc
      361 attctacctg ccagatctcc tgaaacgttc ttcacaatgg agcctgtttt ttccatattg
      421 atccgggata caaagtcttc aagacaaata ctgataattt tcccaataat atcacggctg
      481 aaaatgcctt tttctaccag tctggccatt ctgaatttgt cattctggtt ttcccgccta
      541 tgcctggtat ctataaaatc atatcccata taaccgagac tgactggcaa agatatgagt
      601 cccttcccga cggcccccag aaacccatcc ccattaatat ttacggcctc cagcatatcc
      661 cgggcaattt ctttagcact gtccatatat gctttcctgc ggataatcgc tattgattcc
      721 atcaaacgct atcacaatac catcctcatc attccacgtc agagacaggt acgtcggatg
      781 ctgtctggca gcaagatagc tgagcaactg ccggctcagt accggcagca gttgctggtt
      841 cagcagactc tccacattcc gggcacccgt atccggcagc aggcaggcag atgtcagggc
      901 atcgtacaca cgttcatcaa ccgatgtgct cagtccataa tggcagtgca ggcgttcaca
      961 gacctgtgca agcttcatct ccacaatgat gcgcatggca gactgcgtca gcggacggta
     1021 aatcacggtc tggaaacggg caagcagtgc tggctggaag tggtcgcgca gcagtggacg
     1081 gataagctca tgcagctccg attccgttgt ctccggcttt tcgctcagtt gctgcatcag
     1141 aaggtcactg cccagattgg aggtcatcag aatgaccgta ttacggaagt caatttcacg
     1201 cccctcaccg tcgcgcataa agccccggtc aaacatctgg tagaacagat tcagcacatc
     1261 ccggtgggcc ttttccactt catccagcag caccacgctg taagggcgtt tacgcacagc
     1321 ctccgtcaga ataccgccct gaccgtatcc cacgtacccg ggcggtgacc ccttaagctg
     1381 ggaaaccgta tggggctcct ggtattccga cagattgatg gtaatcagtg atttttcgcc
     1441 gccgtacatc acatctgcca gcgccagtgc cgtttcggtt tttcccacac cactgggacc
     1501 gaccagcagg aacacgccct gggggccgtt accggatgtc agacccgtat tcgccgcacg
     1561 cagacgctgc gcaatgga
//
LOCUS       DAZFLD010000454         1576 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.457, whole genome shotgun sequence.
ACCESSION   DAZFLD010000454 DAZFLD010000000
VERSION     DAZFLD010000454.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1576
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.457"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(1..1507)
                     /locus_tag="ACHKBL_005047"
                     /pseudo
     CDS             complement(1..1507)
                     /locus_tag="ACHKBL_005047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000019324.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MchC protein"
BASE COUNT          449 a          370 c          313 g          444 t
ORIGIN      
        1 ggaaaactta agcataaaat ttttcatatc agaaattgct tcttgtaaaa aaaggggatc
       61 atcgccatga ttatccagca ggatggcaat tttttcgcac atattttctt tcctcagctc
      121 ttccctgggt tgcagccttt tcatggtatg atgttcaatt tctttcagga gtaatgaggc
      181 atttatctga cgtctggtac tgccttgcgt cagccagtgg atggcttggg aaaaacactg
      241 ttgtgcaacg gtgaaataaa catcattctt gatgctacta ttcagtgcct gcttttgatt
      301 atgcctatga tggattagcg tcacaaccaa cttttgcaat ggtgaaccat cctgattgac
      361 aatatcttgg gcatgtgatt tctcgcagta ttgattgagt aatgcaacaa aactgttaac
      421 cagttctttc aatatatcag gtatctcttt ttgtgttata aattctccag gaatacccaa
      481 cagttctaga taacagtaat aacatgcttc atggaaagtt ttctgaaata tctcctcaac
      541 cttcaaacgg gctgcaagag tgaagtcagg gtcaaacata tattttccga tatacaagca
      601 gagatcagac agcccttgca taagtaccat cggttctttt gccagttcag ctaatcttag
      661 tttataccca aaatcagctg gggaatctcc cggaaacaga caactgagtc cgttaaaatc
      721 cattaatctg ccatccattg caatattaga aggagaagtt gtcaatctca ggaaacgtgt
      781 tcgacaaaat gccatttgcc aggcctcacg acgtgccagt tcacataacc cctcaatgca
      841 ataaatccgg ggatcagttc gtgcttcagc atcaatttct tccggtggta caggtagata
      901 tcctggcagc ttgtggatca cagatctaac ccgacaggca tcgtgaatta gcagactgga
      961 atactccggt tttacttgga agtaaggtgc ccgttcaaaa tgcgccggac gaacaacagg
     1021 ctcacgtacc aacagggctc ttcgtgattt cctaccggag cgctcaaatg ccttttcagt
     1081 atagagatct gtaagtaaaa ccgcccgaac ccgcacagca ctataaggta atatttgagc
     1141 cagtacttct ccccacaaag cctcataata ttgcatgaac agcgccgagt gcaccattag
     1201 aatgacgttc gtcagtacct tcgccaacca acggattact tcctattcct ttaacctggt
     1261 agttaccatc aaacccacaa cgtgcaccac ctccattacg ctgaatacca ctgccaccat
     1321 agcgctctgc atggaacact ttggcagaag ctgtactgcc aggatcgata atataactgc
     1381 aggcattttg cagctcgtct ggatttctga catcctctgc acaccagatt aatgaggcct
     1441 tgatctgcct ggcagtgaaa ggcaccaggt tttcattcag ttcagaaagt gaacactgat
     1501 gactcatctg gcagtattct ccgtttcaat tttcataaaa gagtgttcat aaaagagcaa
     1561 gtaagtgtcc tgacag
//
LOCUS       DAZFLD010000455         1573 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.458, whole genome shotgun sequence.
ACCESSION   DAZFLD010000455 DAZFLD010000000
VERSION     DAZFLD010000455.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1573
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.458"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>1573
                     /locus_tag="ACHKBL_005048"
     CDS             <1..>1573
                     /locus_tag="ACHKBL_005048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HFO5821761.1"
                     /translation="LLIQLERNGHWVTEKDITINGKTTSQYLMSVILNNLPERPFNIR
                     MVRETADSTTDQLQNRTLWSSYTEIIDVKQCYPNTAIVGLQVDAEQFGGQQLTVNYHI
                     RGRIIQVPSNYDPEKRTYSGIWDGSLKPAYSNNPAWCLWDMLTHPRYGMGKRLGAADV
                     DKWALYAIGQYCDQTVPDGFGGTEPRMTFNAYLSQQRKVWDVLGDFCSAMRCMPVWNG
                     QTLTFVQDRPSDVVWPYTNSDVVVDDNGVGFRYSFSAMKDRHTAVEVNYTDPQNGWQT
                     STELVEDPEAILRYGRNLLKMDAFGCTSRGQAHRAGLWVIKTELLETQTVDFTLGSQG
                     LRHTPGDIIEICDNDYAGTLTGGRILSIDAASRTLTLDREVTLPEAGTSTVNLINGSG
                     KPVRVDITAHPAPDRIQVSVLPDGMETYGVWGLSLPLLRRRLFRCVSIRENTDGTFAI
                     TAVQHVPEKEAIVDNGARFEPMSGSLNSVIPPAVQHLTVEVSASDGQYMALAKWDTPR
                     VVKGVRFSLRLTSGSG"
BASE COUNT          339 a          442 c          507 g          285 t
ORIGIN      
        1 ctgctgattc agcttgagcg taacgggcac tgggtgacgg agaaggatat caccattaac
       61 ggcaaaacca cctcgcagta cctgatgtcg gtgattctga ataatctccc tgagcgcccc
      121 tttaacatcc ggatggtcag ggagacggcg gacagcacca cggaccagct gcagaacaga
      181 acgctgtggt cgtcatacac cgaaatcatc gatgtgaaac agtgctaccc gaacacggcg
      241 attgtggggc tgcaggtgga tgcggagcag tttggtggcc agcagctgac ggtgaactac
      301 catatccgtg gtcgcatcat tcaggtgccg tcaaactatg acccggaaaa acgcacctac
      361 agcggtatct gggacggcag tctgaaaccg gcatacagca ataacccggc ctggtgcctg
      421 tgggacatgc tgacccaccc gcgctacggc atgggaaaac gcctgggggc cgcggacgtg
      481 gacaagtggg cactgtatgc catcgggcag tactgtgacc agacggtccc ggatggtttc
      541 gggggaacag agccgcggat gactttcaat gcgtacctgt cacagcagcg taaggtctgg
      601 gatgtcctgg gggatttctg ctcggcgatg cgctgtatgc cggtatggaa cggccagacg
      661 ctgacgttcg ttcaggaccg tccgtcggat gtggtgtggc cgtacaccaa cagcgatgtg
      721 gtggtggatg ataacggcgt ggggttccgc tacagcttca gtgccatgaa ggaccggcac
      781 acggcggtgg aggtgaatta caccgacccg cagaacggct ggcagacctc cacggaactg
      841 gtggaagacc ctgaagccat actgcgctac ggacgcaacc tgctgaagat ggatgcgttc
      901 ggctgtacca gccgcggtca ggcccaccgt gccggactgt gggtgataaa gaccgaactg
      961 ctggaaacgc agacggtgga tttcacgctc gggtcacagg ggctgcgtca cacgcccggt
     1021 gacatcattg aaatctgtga taacgactac gccgggaccc tgaccggcgg acgcatcctg
     1081 tccatcgatg ccgccagccg taccctgacg ctggaccgtg aggtgacact gccggaagca
     1141 gggacatcga cggtgaacct gattaacggc agcggtaagc cggtgcgcgt ggacatcact
     1201 gcacaccccg cccctgaccg gatacaggtc agcgtcctgc cggatggcat ggagacatac
     1261 ggtgtgtggg gactctccct gccgttactg cgtcgtcgcc tgttccgctg tgtttccatc
     1321 cgggaaaaca cggacggcac ctttgccatc acggcagtgc agcacgtacc ggaaaaagaa
     1381 gccatcgtgg ataacggggc ccgctttgag ccgatgtccg gctcactgaa cagcgtcatc
     1441 ccgccggcag tgcagcacct cacggtggag gtaagtgcct cagacggcca gtatatggcg
     1501 ctggcgaaat gggacacgcc gcgggtggtg aagggcgtgc gcttcagtct gcgcctgacc
     1561 agtggcagtg gtg
//
LOCUS       DAZFLD010000456         1560 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.459, whole genome shotgun sequence.
ACCESSION   DAZFLD010000456 DAZFLD010000000
VERSION     DAZFLD010000456.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1560
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.459"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            416..>1560
                     /locus_tag="ACHKBL_005049"
     CDS             416..>1560
                     /locus_tag="ACHKBL_005049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S6 family peptidase"
                     /protein_id="HFO5821762.1"
                     /translation="MNKIYSLRVNDRKELVAVPETVGGRKKSSGIPGAGMLSRLLLAS
                     GAVAGVLFSSPALASVVGNTLPWQTYRDFAENKGAFHAGATNIPLYGKNGAVSGRLDK
                     APMMDFSVVDQILGVATLISPQYVAGVKHNGSYNTVRFGYADDTTYRLVDRNEHWRDF
                     HTPRLNKLVTEVAPVSVTDAGTGKGVYQNRSRYPVFYRMGSGTQYTGAASGALTRIAD
                     AYVWRTGGTVGSPVISDWSLVSNPGNLYQGVNGPLASYGTSGDSGSPLFAWDAVKKQW
                     VFVAVLHGYAGENGKTNWFTVIPAGDVNNTIKQDSSGTVVPAVAGGDIVWNYSKGSGE
                     GTLSQDGKVWKMNGFRGGSLNDGKDITFGGKGTVVLKDDVVQGAGSLT"
BASE COUNT          363 a          297 c          503 g          397 t
ORIGIN      
        1 ggtcttaagt acaggagtgt gaagcacctg gttggacgtg cgtagccgga atgcatattc
       61 gtgagtcggg taatgagagt ggaacggtgg cagtgagaat ggaacagaaa aaaatcagat
      121 gcggaaagcg gaaatacaca gcgggtgcga tgtgcttcat gctgtttgta tggctggggg
      181 gtgtggtgac attctgtgtg ctggccgctc tgataatgag gtgaaaacgg aagagagttt
      241 acttccttaa ttcgaacttt ctgataactg agaacttgtt aaatgcctgt gggtttatgt
      301 atttaaatca attggttaat ggtgatttta tctttatcag tcaaagttcg ttttagtatg
      361 atgattgtgc ccgctgttta ttgttgtccg ggagaaaata cagggaatcc aggggatgaa
      421 caaaatatac tcactcagag ttaatgacag aaaagaactg gttgctgtac cggaaacggt
      481 ggggggcagg aaaaagtcgt ccggcattcc cggtgcgggg atgctttccc gtcttcttct
      541 ggcctcagga gccgttgccg gcgttctgtt ttcttctcct gcgctggcat ccgttgtcgg
      601 gaatactctg ccatggcaga cctacaggga ttttgctgaa aacaaagggg cgtttcatgc
      661 cggggccaca aatattcctc tttatggtaa gaatggggct gtcagtgggc ggctggacaa
      721 ggctccgatg atggatttca gtgttgtgga ccagatactg ggggtggcaa cactgatttc
      781 tccgcaatat gtggcggggg ttaagcataa cggcagctac aatacggtgc gttttggtta
      841 tgcggacgat acgacatatc gccttgttga ccgtaatgag cactggcgtg attttcatac
      901 tccccggctg aacaaactgg tgacagaagt tgccccggtg agcgtcacgg atgccggaac
      961 cggcaagggg gtataccaga accgcagtcg ttatccggtg ttttatcgta tggggagtgg
     1021 aacgcagtac accggagctg cttccggtgc gctgaccaga atagcggatg cttacgtctg
     1081 gaggactggc gggacggtgg gaagtccggt catttctgac tggagtctgg tgtcaaaccc
     1141 gggaaatctt taccagggtg ttaatggtcc gctggcgtct tacgggacat caggagacag
     1201 tggctcaccg ctttttgcct gggatgcggt gaagaagcag tgggtatttg ttgctgtact
     1261 tcatggttat gccggtgaga acgggaagac gaactggttt acggtgattc ctgcagggga
     1321 tgtgaataac acgataaagc aggacagttc aggcaccgtc gtgccggcag ttgccggtgg
     1381 tgacattgtg tggaattaca gtaagggaag cggagaggga accctttctc aggatgggaa
     1441 ggtatggaag atgaacggct tcaggggagg gagtctgaat gacgggaaag atatcacctt
     1501 tgggggtaag ggcacggttg ttctgaagga tgatgttgtc cagggggcgg gttcgctcac
//
LOCUS       DAZFLD010000457         1543 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.460, whole genome shotgun sequence.
ACCESSION   DAZFLD010000457 DAZFLD010000000
VERSION     DAZFLD010000457.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1543
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.460"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            137..>1543
                     /locus_tag="ACHKBL_005050"
     CDS             137..>1543
                     /locus_tag="ACHKBL_005050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemagglutinin"
                     /protein_id="HFO5821763.1"
                     /translation="MHDFYVIPTEWNENKGEKRVYMNLRKKHICIAISLVLNPFPASL
                     AAQNTNVVEVDESSQGYDVHGDNWKSIEIGTTQEVKASPDKNINFDHNSPVAEGRNSI
                     AIGTSAEAGKEMTDKMAGEAIAIGAKAKATKEQSIAIGGDTKATGWGAIVIGGDDLTP
                     LHGDRFGDKEIPNNYSSSEASGDGSIVVGSRSTAAGDLSVSLGSRSTAAGDLSVSLGS
                     ISSANGTAANALGATAEANGDMATAVGFNALANASNALAVGSQSSAGGEESLAIGSQS
                     TTGKKSTVALGQGATASAAEGNVAIGADSVDKAATPVSGATIKLKNGGTIEYRGFAGD
                     KAASVVSVGDAGKERQIVNVGAGAVSEKSTDAINGSQLYAIAKTLKDDLDAINPTWKL
                     KDSTGKEKEVGSKNGGTTGNEVTVTGKGDIDVTVDGNGLSIDGTKLAENIKKDAIKIK
                     TGDGKTLTKKLGEEVEFND"
BASE COUNT          462 a          277 c          429 g          375 t
ORIGIN      
        1 atgttttatg tttgcattat tataaatcac tttattgcct tttgtctgct ttttaatggt
       61 tcccgttttg ttcacttctg gctttataaa aaatggatgc tgaagaaatg aaggcagtcc
      121 gccttacata atattcatgc atgattttta tgtcattccg actgagtgga atgaaaataa
      181 aggagaaaag agggtatata tgaacctcag aaaaaaacat atatgcatag ccatatcact
      241 tgttcttaat ccgttccctg catcactggc agcacaaaac actaatgtag ttgaagttga
      301 tgaaagcagt caggggtatg atgtgcatgg agataactgg aagagcattg aaatagggac
      361 aacgcaggaa gttaaagcat cccccgataa aaatattaat ttcgatcata attctcctgt
      421 tgcagaagga cgtaactcta tcgcgattgg taccagtgca gaggctggta aagaaatgac
      481 ggataaaatg gcaggggaag ccatagctat tggtgccaag gcgaaagcaa ctaaagaaca
      541 gtcaattgca ataggtggcg ataccaaagc taccggatgg ggggctattg ttattggtgg
      601 agatgattta acccctttgc atggtgacag attcggtgat aaagaaattc cgaataatta
      661 ctcgtcatct gaagcatcag gagacggaag tattgttgtg ggctcaagga gcacagctgc
      721 aggagacctg tctgtgtccc tgggctcaag gagcacagct gcaggagacc tgtctgtgtc
      781 cctgggctca ataagctctg ccaacgggac ggctgctaat gcactgggag ccacggcgga
      841 agctaacggg gatatggcga ctgcggttgg ttttaatgcg ctggctaatg ccagtaatgc
      901 gctggctgtt ggtagtcagt cctctgccgg cggagaggaa tccctggcta tagggagtca
      961 gagtacgacg ggcaaaaagt ccacggttgc tctggggcag ggagctacag cctctgccgc
     1021 ggaggggaac gtggccatcg gtgctgattc agtggacaag gcagcaactc cggtttccgg
     1081 cgctaccatt aagttaaaga atggcgggac tattgaatac aggggctttg ccggagataa
     1141 ggctgcatca gtagtatctg ttggtgatgc gggtaaagag cgtcagattg ttaacgtggg
     1201 ggcgggtgct gtatctgaga aaagtacaga tgccattaac ggtagtcagt tatatgcgat
     1261 agcgaagaca ctgaaagatg accttgatgc cattaatccg acgtggaagt taaaagacag
     1321 cacaggaaaa gaaaaagagg tcggcagtaa aaatggcggg actacaggta atgaagtgac
     1381 cgtcaccggt aaaggggata ttgatgttac tgttgatgga aatgggctga gtattgatgg
     1441 cacaaagctt gctgaaaaca tcaaaaaaga tgccatcaag attaaaacgg gagatggtaa
     1501 aacactaacg aaaaaacttg gtgaagaagt tgagtttaat gat
//
LOCUS       DAZFLD010000458         1539 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.461, whole genome shotgun sequence.
ACCESSION   DAZFLD010000458 DAZFLD010000000
VERSION     DAZFLD010000458.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1539
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.461"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            204..539
                     /locus_tag="ACHKBL_005051"
     CDS             204..539
                     /locus_tag="ACHKBL_005051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000157082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobC"
                     /protein_id="HFO5821764.1"
                     /translation="MASKKFYSDDDIQLAKAALSELPDLTAQRKTLRDFLDAIRDDII
                     VLVRTKGYTLADVRDTLKNAGYEVGEKALRDIIREAESKKPSRRSSSKRAPKKTDSGR
                     NDGIDMNNN"
     gene            complement(624..1472)
                     /locus_tag="ACHKBL_005052"
     CDS             complement(624..1472)
                     /locus_tag="ACHKBL_005052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HFO5821765.1"
                     /translation="MNQTLPTADLNTDGTTDVIPSVAIDRIIAQRNEGISLFMQAMEC
                     LATARRILLDASGDIFLYGFEDCVTDSVRCMDKPEEAKKNITRLADRKIWDRLMTDTG
                     MYTFMSSCQRDEWNSQLMSDTCPEITLDNVLATFRHLNASKMQTFEHGLIDVYRKLSW
                     DYRTNNPCRLGKKIIIENLLYRWSNGRVTLDCSGREALDDLVRPFYLLEGRNVPDFRN
                     SIGAQYGEFLGNGDNVGELFEGEYFTVRGYQKGTVHIVFKRPDLVEKLNDIIARHYPG
                     ALPPRV"
BASE COUNT          409 a          384 c          356 g          390 t
ORIGIN      
        1 aagccctgac caggaggcat ttgtctgatt gcgcgtgcgc ggtccgacaa atgcacatcc
       61 tgtcccgttt ttcaggacat tatagcctgt tatttttgtg catcatagtc ttatgcagac
      121 ggcagaggca gggcgcagcc ctgccaggag tcagtttttg atgataaaat caggcttcaa
      181 catgaaacag agagaaagtg ccaatggctt ctaaaaaatt ttactcagat gatgacatcc
      241 agctggcaaa agctgcttta tcggaattgc cagatctcac cgctcaacgg aaaacattgc
      301 gtgattttct tgatgccatt cgtgatgaca ttattgtcct ggtaaggaca aagggttata
      361 cactggcaga tgttcgtgac acgctcaaaa atgcagggta tgaagtagga gagaaggcac
      421 ttcgtgacat tattcgggaa gcagaatcga aaaaaccatc ccgccgttct tcctctaaaa
      481 gagcacctaa aaagacagat tcaggcagga atgatggcat cgatatgaac aacaattgat
      541 gtgacaatgt cacatcgtgt tctgagatta tgatgattac agaaaaaaag ccccggcata
      601 aataccaggg cttttctgtt taatcagact cgtggcggca atgcaccagg ataatgccgt
      661 gcaataatat cattcagttt ttcaacaagg tcaggacgct taaagacaat gtgtacggtc
      721 cctttctggt agccacgcac cgtaaaatat tccccttcaa acaactcacc gacattgtcg
      781 ccgttcccga gaaattcacc atactgcgcc ccgatgctgt tcctgaagtc aggaacgttg
      841 cgcccctcca gcagataaaa cggacgtacc aggtcatcca gtgcctcccg tccgctgcag
      901 tccagcgtca cacgcccgtt actccagcgg tacagcaggt tttcaataat gattttcttt
      961 cccagacggc agggattatt ggttctgtaa tcccatgaca atttccggta gacatcaatc
     1021 agtccatgtt caaatgtctg catcttactg gcattcagat ggcgaaaagt tgccagtaca
     1081 ttgtccaggg tgatttcagg acaggtgtcg ctcatcagct ggctgttcca ctcatcacgc
     1141 tggcatgaac tcatgaatgt gtacatgcca gtatccgtca tcaggcgatc ccagattttc
     1201 cggtcggcaa gacgggtgat attctttttt gcttcttccg gtttatccat gcatcgaacg
     1261 gagtcagtca cgcagtcttc aaacccgtag agaaaaatgt cacctgacgc atcaagcaga
     1321 atcctgcgtg ctgtcgccag gcattccatc gcctgcatga acagtgaaat accttcgtta
     1381 cgctgcgcga tgatgcggtc gatagccaca gacggaataa catctgtcgt gccgtcagta
     1441 ttcaggtcag cagtgggtaa agtctgattc atcacattgt ctcctcatcg tcacgactgc
     1501 cgggtcagcc aggtcatttc ccggaggagc gaagctcct
//
LOCUS       DAZFLD010000459         1534 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.462, whole genome shotgun sequence.
ACCESSION   DAZFLD010000459 DAZFLD010000000
VERSION     DAZFLD010000459.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1534
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.462"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..246
                     /locus_tag="ACHKBL_005053"
     CDS             <1..246
                     /locus_tag="ACHKBL_005053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5821766.1"
                     /translation="PAGNLLYRSALAFIQALIALVPSKKILDLDKITDALRRRYVHLE
                     QLEYAELRNALACALSTCYNRKYLIKVAKGKYLPVDD"
     gene            328..675
                     /locus_tag="ACHKBL_005054"
     CDS             328..675
                     /locus_tag="ACHKBL_005054"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO5821767.1"
                     /translation="MPGQPIPSELKEKGLIWLLPPYCWSHRQIARKLKISASVVSKWR
                     YELVEQGLLPENEKITSVDVEDWTPERRFSVVLETATLSEMELADYYRRMGLFVEQVK
                     EWRAISIQAHEKN"
     gene            848..1012
                     /locus_tag="ACHKBL_005055"
     CDS             848..1012
                     /locus_tag="ACHKBL_005055"
                     /inference="COORDINATES: protein motif:HMM:NF024776.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO5821768.1"
                     /translation="MERRKIIALVREAVSNGARKSQACRCIGISIRTLQRWTDGNVIT
                     TDVSVNGAPY"
     gene            complement(1016..1459)
                     /locus_tag="ACHKBL_005056"
                     /pseudo
     CDS             complement(1016..1459)
                     /locus_tag="ACHKBL_005056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080195.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          425 a          354 c          367 g          388 t
ORIGIN      
        1 cctgctggaa accttcttta cagaagtgca ttagctttta tacaggcctt gattgcatta
       61 gtcccatcaa agaaaatact ggacttggat aagataacag atgctctgcg taggcggtat
      121 gttcatcttg agcaattaga atatgccgaa ttaagaaatg ctttagcttg tgcattgtca
      181 acttgttata atagaaaata tctcattaaa gttgcaaaag gaaaatattt acctgttgac
      241 gactaggtgc gtattaccgt gaaggagatc ggtgagtaac cccctgtgtc agcatagttg
      301 tcgcctcaat tttttaagga gataacaatg ccaggacaac caataccaag tgagctcaaa
      361 gagaaaggac tgatatggct tttaccacca tactgctggt cacatcgtca gatagcccgt
      421 aagctaaaga tcagtgcctc agttgtatca aaatggagat atgagttagt ggagcaaggg
      481 cttttacctg aaaatgaaaa gattacctcc gttgatgttg aagactggac accggagcgt
      541 cgattctcag ttgttcttga gacggctacg ctgtcagaaa tggagcttgc tgattattac
      601 cgacgcatgg gattgtttgt tgaacaggtt aaagaatggc gggctatctc aattcaggcg
      661 catgaaaaaa attgacggaa aatattcgaa cagacagggc attgcgtgac gcaaaaaata
      721 agttacgaga actgtagaaa gagcttaagc gtaaagataa agctctggcg gaagctgcgg
      781 ccctgcttat attgagggaa aagttcaatg ccctctggga caacagcgag gaagactgac
      841 ccctctcctg gagcggcgta aaattattgc ccttgtccgc gaggccgtct ccaacggcgc
      901 ccggaaaagt caggcttgta ggtgtatcgg tatttcaatt cggacacttc agcgctggac
      961 tgatggcaac gttattacca cggatgtaag cgtcaacgga gcaccgtatt gacgcttatt
     1021 tattggtgag tactacgttc catggcagga gttcatcaac tcggttggaa ggccattccg
     1081 gcagtacgct caggataagg cgcagatacg cttccggatc gataccgttc agacggcagg
     1141 tgccgatcag cccgtacaac agtgctccac gctcgccgcc gtgatcgctg ccaaagaaca
     1201 taaagttttt ctttccgaga cagactgcac gaagcgctct ttccgcagca ttattatccg
     1261 cctccgccag accgtcatca ctgtaataac agagggcatc ccactgattc agtacatagc
     1321 tgaacgcttc gcccagtctg gattttttcg acagcgtacc attcttctcc accatccatt
     1381 catgcagcga cgtcagtaac actttgcttc gctgctgcct gacggaaaga cgctctgact
     1441 ccggtaatcc ccgtatttcg taagcgtaaa ccgaccgccg tatgtagcca ttaagccagt
     1501 attggtaacg taggtgccca ccttttcaaa ctag
//
LOCUS       DAZFLD010000460         1533 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.463, whole genome shotgun sequence.
ACCESSION   DAZFLD010000460 DAZFLD010000000
VERSION     DAZFLD010000460.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1533
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.463"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            108..1514
                     /gene="gndA"
                     /locus_tag="ACHKBL_005057"
     CDS             108..1514
                     /gene="gndA"
                     /locus_tag="ACHKBL_005057"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HFO5821769.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIV
                     ENPGKKLVPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLTNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQSAGDKAEFIEKVRRALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
BASE COUNT          394 a          363 c          378 g          398 t
ORIGIN      
        1 aactttgttt ttaccgtcaa tatcatatat ataatacttt atttcaagta aactttttaa
       61 gttattctca taccgcacat gcggtaaaac cctgacagga gtaaacaatg tcaaagcaac
      121 agatcggcgt cgtcggtatg gcagtgatgg ggcgcaacct cgcgctcaac atcgaaagtc
      181 gtggttatac cgtctctatt ttcaaccgtt cccgtgaaaa gacggaagaa gtgattgtcg
      241 aaaatccagg caagaaactg gttccttact atacggtgaa agagtttgtt gaatctctgg
      301 aaacgcctcg tcgcatcctg ttaatggtga aagcaggtgc aggcacggat gctgctattg
      361 attccctcaa gccatacctc gataaaggtg acatcatcat tgatggtggt aataccttct
      421 tccaggacac cattcgtcgt aaccgtgagc tttctgccga aggctttaat tttatcggta
      481 ccggtgtttc cggtggtgaa gaaggtgcgc tgaaaggtcc ttccattatg cctggtgggc
      541 agaaagaagc ctatgaactg gttgcaccga tcctgaccaa aatcgccgca gtggctgaag
      601 acggtgagcc atgcgttacc tatattggtg ccgatggtgc aggtcactat gtgaagatgg
      661 ttcacaacgg tattgaatac ggcgatatgc agctgattgc tgaagcctat tctctgctta
      721 aaggtggcct gaatctcacc aacgaagaac tggcgcagac gtttaccgag tggaataacg
      781 gtgaactaag cagctacctg atcgacatca ccaaagatat cttcaccaaa aaagatgaag
      841 acggtaacta cctggttgat gtgattctgg atgaagcggc taacaaaggt accggtaaat
      901 ggaccagcca gagtgcgctg gatctcggcg aaccgctgtc gctgattacc gagtctgtgt
      961 ttgcacgtta tatctcttct ctgaaagatc agcgcgttgc cgcatctaaa gttctctctg
     1021 gtccgcaagc tcagtcagca ggcgacaagg ctgagttcat cgaaaaagtt cgtcgtgcgc
     1081 tgtatctggg caaaatcgtt tcttacgctc agggcttctc tcagctacgc gcagcgtctg
     1141 aagagtacaa ctgggatctg aactatggcg aaatcgcgaa gattttccgt gctggctgca
     1201 ttatccgtgc gcagttcctg cagaaaatca ccgatgctta tgccgaaaat ccgcagatcg
     1261 ctaacctgct gctggctcct tacttcaagc aaattgccga tgactaccag caggcgctgc
     1321 gcgatgtcgt tgcttatgca gtacagaacg gtatcccggt tccgaccttc gccgctgcgg
     1381 ttgcctatta tgacagctac cgtgccgctg ttctgcctgc gaacctgatc caggcacagc
     1441 gtgactattt cggtgcgcat acttataagc gcattgataa agaaggtgtg ttccataccg
     1501 aatggctgga ttaatctgat ttaatcaaca ata
//
LOCUS       DAZFLD010000461         1531 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.464, whole genome shotgun sequence.
ACCESSION   DAZFLD010000461 DAZFLD010000000
VERSION     DAZFLD010000461.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1531
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.464"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1157
                     /locus_tag="ACHKBL_005058"
     CDS             <1..1157
                     /locus_tag="ACHKBL_005058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="conjugal transfer protein TraG N-terminal
                     domain-containing protein"
                     /protein_id="HFO5821770.1"
                     /translation="SGVISSIGDQTAAAPVWWVVVHKLSKGVTQAAVASIPCRPDLRQ
                     LRFEIQRTFIENRALADELQDFTNDCYSLALYMWKQRDQGQSKDKKVLDDISWIGSAT
                     FMQGDYGTLQSKMPRAAFPWNTARDSGRPDTGRGGYPTCREWWSDSSVGLKKRVMEEV
                     SDHYWLRLSAAMKMIGSSQVDYKEEVIKRLVSPANLTLSQGGEVYRGYGGNADMTTMN
                     AVARIAGTAGSAVAALGAFPALDAMRQALPMVQSIILMAIYIMIPLILAFAAYELSTL
                     ITLTFVVFAVHFLTFWWELARWLDSWMLTALYSSDTHTRFNMMGFQNTSDDLIMNLVM
                     GTMFLVLPAVWLGALSWAGVSVGTVMGDAINKGTSNVSKAAAKSGDLANK"
     gene            complement(1208..1447)
                     /locus_tag="ACHKBL_005059"
     CDS             complement(1208..1447)
                     /locus_tag="ACHKBL_005059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001058544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5821771.1"
                     /translation="MNNESEKAMWRCKPVDNPWTFTSLLKKAVCAVLPDFTPAQDKPQ
                     NMFSLSNEKDTDYSVPMRPFALYDPWKHHHDNADF"
BASE COUNT          377 a          292 c          463 g          399 t
ORIGIN      
        1 acagtggggt gatcagcagt ataggggatc agactgctgc tgccccggta tggtgggtgg
       61 tggttcataa attgtcgaaa ggtgtcaccc aggcggcagt ggcttcgatt ccgtgccggc
      121 ctgatttgcg ccagttgcgt tttgagatcc agcgaacgtt tatagaaaac agggcacttg
      181 ctgatgagct gcaggacttt acgaacgact gttattcact ggcactgtat atgtggaaac
      241 agcgtgatca ggggcagagt aaagataaaa aagtgcttga tgatatctcc tggataggga
      301 gcgccacatt catgcagggg gattacggga cgctgcagtc aaagatgccg cgggctgcgt
      361 tcccgtggaa tacagcacgt gacagcggac gaccggatac ggggcgggga ggctatccga
      421 catgccggga gtggtggagt gacagtagtg tggggctgaa aaaacgggtc atggaggagg
      481 tcagtgatca ttactggtta cgtctgtcag ctgcaatgaa gatgattggc agtagtcagg
      541 tggattataa ggaggaggtg atcaagcgtc tggtgagccc ggcaaacctg acgctctctc
      601 aggggggaga ggtgtatcgt ggctatggtg gcaatgcaga tatgacgacc atgaatgcgg
      661 tggcccggat tgcgggaacg gcgggcagtg cggtagcagc actgggtgcg tttccggcac
      721 tggatgcaat gcggcaggca ctgccgatgg tacagtcgat aatactgatg gcgatttaca
      781 tcatgatacc gctgattctg gcgtttgcgg catatgaact gtcgacgtta atcacactga
      841 cgtttgtggt gtttgcggta cactttctga cattctggtg ggagcttgca cggtggcttg
      901 acagctggat gctgacggcg ctgtacagtt cggatacaca tacccggttc aatatgatgg
      961 gattccagaa cacatcggat gatctgataa tgaatctggt gatggggacg atgtttctcg
     1021 tcttgccggc agtgtggctg ggagcgttgt cctgggctgg ggtaagtgtc ggaacggtta
     1081 tgggggatgc aattaacaaa ggaacaagta atgtatctaa ggcagcagca aaaagtggag
     1141 atttagctaa taagtaaaat tcttagggtt tccttagctg ggataattgg ggaaaccctt
     1201 gcttttctta gaaatcagca ttgtcatggt gatgtttcca cgggtcatat aatgcaaagg
     1261 gtctcattgg tactgaataa tcagtatctt tttcattact tagtgaaaac atattttggg
     1321 gtttatcttg agcaggtgtg aaatctggta aaacggcaca gactgctttt ttcagcaggg
     1381 aagtgaaggt ccatggatta tccacaggct tacaacgcca cattgctttt tcagattcgt
     1441 tattcatatc tataccctca gcagcaggat aaataacaca gtgttatcat acgtcttgta
     1501 tgataaatgt gcaatcacat gtcttccatt t
//
LOCUS       DAZFLD010000462         1523 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.465, whole genome shotgun sequence.
ACCESSION   DAZFLD010000462 DAZFLD010000000
VERSION     DAZFLD010000462.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1523
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.465"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>1523
                     /locus_tag="ACHKBL_005060"
     CDS             <1..>1523
                     /locus_tag="ACHKBL_005060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFO5821772.1"
                     /translation="NWNDNGDLMRISGPRQTREYGYSATGRLASVRTTTSDLDIRIPY
                     ATDPAGNRLPDPELHPDSTLTVWPDNRIAEDAHYVYRHDEYGRLTEKTDRIPAGVIRT
                     DDERTHHYHYDSQHRLVFHTRIQHGEPLVESRYLYDPLGRRMAKRVWRRERDLTGWMS
                     LSRKPEVTWYGWDGDRLTTVQTDSTRIQTVYQPGSFTPLIRIETDVTELEKAQRRSLA
                     EKLQQEGSEDGHGVVFPAELVRLLDRLEEEIRTDRVSSESRAWLAQCGLTVEQLEKQV
                     EPEYTPARKVHLYHCDHRGLPLALISEDGNTVWSAEYDEWGNLLNEENPHHVYQSYRL
                     PGQQHDEESGLYYNRNRYYNPLLGRYITQDPIGLAGGWSLYAYPLNPVNGIDPLGLSP
                     ADVALMRKKEQLNHQRAWDILSDTYDDMKRLNLGGTDQFFHCMAFCRVSKLNDAGVSR
                     SAKGLGYEKEIRDYGLNMFGMYGRKVKLSHSEMIEDNKKDLAVNEHGLTCPLTQDCS"
BASE COUNT          397 a          364 c          484 g          278 t
ORIGIN      
        1 ataactggaa tgacaacggc gacctgatgc gcatcagcgg cccgcgacag acgcgggaat
       61 acggctacag tgccacgggc aggctggcga gcgtgcgcac gaccacatca gacctggata
      121 tccgcatccc gtatgccacc gacccggcgg gaaaccgcct gccggacccg gagctgcacc
      181 cggacagtac actcacagtg tggccggata accgcatcgc ggaggatgcg cactatgtct
      241 accgccacga tgaatacggc aggctgacgg agaagacgga ccgcatcccg gcgggtgtga
      301 tacggacgga cgacgagcgg acccaccact accactacga cagccagcac cgcctggtgt
      361 tccacacgcg gatacagcat ggcgagccac tggtcgagag ccgctacctc tacgacccgc
      421 tgggacggcg aatggcgaaa cgggtatggc ggcgggagcg tgacctgacg gggtggatgt
      481 cgctgtcgcg taaaccggag gtgacgtggt acgggtggga cggcgacagg ctgacgacgg
      541 tgcagactga cagcacgcgt atccagacgg tgtaccagcc gggaagcttc acaccgctca
      601 tccgcatcga aacagacgtg accgaactgg agaaagcgca gcggcggagc ctggcggaga
      661 agctccagca ggaagggagc gaggacggac acggcgtggt gttcccggct gaactggtgc
      721 ggctgctgga caggctggag gaagaaatcc ggacagaccg cgtgagcagt gaaagccggg
      781 cgtggcttgc gcagtgcggg ctgacggtgg agcaactgga aaaacaggtg gagccggaat
      841 atacaccggc gcgaaaagtt catctttacc actgcgacca ccggggcctg ccgctggcgc
      901 ttatcagcga agacggcaat acggtgtgga gcgcggagta tgatgaatgg ggaaacctgc
      961 ttaatgagga gaacccgcat catgtgtatc agtcataccg tctgccgggg cagcagcatg
     1021 atgaggagtc ggggctgtac tacaaccgga accggtacta caatccgttg ctgggacgat
     1081 atattactca ggacccgatt gggctggcgg ggggatggag tctgtatgcg tatccgctga
     1141 atccggtgaa tggtattgat ccattaggat taagtcctgc agatgtagcg ttaatgcgaa
     1201 aaaaagaaca attaaaccat caaagagcat gggatatatt atctgatact tatgatgata
     1261 tgaagagatt aaatttaggt gggactgatc aatttttcca ttgtatggca ttttgtcgag
     1321 tgtctaaatt aaatgacgct ggtgttagcc gatcagcgaa agggctgggt tatgaaaaag
     1381 agattagaga ttacgggtta aatatgttcg gtatgtacgg cagaaaagta aagttatccc
     1441 attctgagat gattgaagat aataaaaaag acttggctgt aaatgaacat gggttgacat
     1501 gtccattaac acaagattgc tca
//
LOCUS       DAZFLD010000463         1519 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.466, whole genome shotgun sequence.
ACCESSION   DAZFLD010000463 DAZFLD010000000
VERSION     DAZFLD010000463.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1519
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.466"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            85..>1519
                     /locus_tag="ACHKBL_005061"
     CDS             85..>1519
                     /locus_tag="ACHKBL_005061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000009550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HFO5821773.1"
                     /translation="MSFNPTDEQYAIINWQGQQLVVNAFSGTGKTSTLVQFARASPDS
                     RMLYLAYNRAIRDEAERKFPFNVECRTSHQLAYARVGRHYRHRLVPGLRITDVARKLN
                     TRYWALARVAGTGLNHFICSADAVPGLHHLPDKDEMRGVPPADALRAVQLLWNEMSNP
                     GGTFPVTHDTYLKLFQLSGADLSHRWDTILFDEAQDANPVTTALVLGQKCRVVLVGDC
                     YQQIYRFRGANNALSHPALKNADRLWLTQSFRFGPAVARMANLLLQREGETREVKGCG
                     GDDEVLLKCHAREHLQGHYTVLSRTVAGVIGTALMAAMKGQKVYWVGGIEGYRTGELE
                     DLYWFQVDMPERMHSDRLRRDYRNFEEYKYIAKSTKDVEMNQSLRLLELCFPLPKKLE
                     LLRQYTVTNEQDADITVSTAHRSKGLEWPVVMLDEDFQDITDPLMTEAERHDETCLLY
                     VAVTRALRTLVLNDLMHQLAEEQQKTEG"
BASE COUNT          353 a          331 c          470 g          365 t
ORIGIN      
        1 ttctgttgct gtacagagtt atttgtggtg ggcatacccc tgatgcatca gattacggaa
       61 atgtttttcc ggagaagaga atgcatgtct tttaatccca cagatgaaca gtacgccatt
      121 attaactggc aggggcagca actggttgtg aatgcgtttt ccggcacagg gaagacgagt
      181 acgctggtgc agtttgcccg ggccagtccg gacagccgta tgctctatct tgcctataac
      241 cgggcaatac gtgatgaggc agagcgtaaa tttcccttta acgtggaatg cagaacgtca
      301 catcagctgg cgtatgcccg ggttggaaga cactaccgtc atcggctggt tccgggatta
      361 cgtattactg atgtggcgag aaagctgaat acacgatact gggctctggc acgggttgca
      421 gggacggggc tgaatcactt tatctgttct gccgatgcgg taccgggcct gcatcacctg
      481 ccggataagg atgagatgcg gggtgttccg ccagcagatg cgcttcgggc ggtgcagttg
      541 ctctggaacg aaatgagcaa ccccgggggg acgttcccgg tgacgcacga tacttatctg
      601 aaactcttcc agttgtccgg tgcagacctg tctcaccgat gggacaccat cctgtttgat
      661 gaggcgcagg atgcgaatcc ggtgaccact gctctggttc tggggcagaa gtgtcgggtg
      721 gtccttgttg gagattgtta tcaacagatt taccgctttc ggggtgcgaa taatgcgctt
      781 tcccatcctg cactgaagaa tgcggaccgg ttgtggctga cacagagttt ccgttttggt
      841 ccggcggtgg ctcgtatggc aaatctgctt ctgcagcgtg aaggtgaaac acgagaggtg
      901 aaaggttgtg gtggtgatga tgaggtattg ctgaaatgcc atgcgcgtga acatctgcag
      961 gggcattaca cggtactcag tcgtactgtc gcgggtgtga ttggtactgc gctgatggcg
     1021 gcaatgaaag ggcaaaaggt ttactgggtg gggggaatcg aaggttacag gaccggggaa
     1081 cttgaggatc tctactggtt ccaggttgat atgccggaac ggatgcattc agacaggctg
     1141 cgccgggact acaggaattt tgaagaatat aaatatattg caaaatccac gaaggatgtg
     1201 gagatgaatc agtcccttcg gcttctggag ttgtgttttc cgttaccgaa aaaactggaa
     1261 cttctccggc agtatacggt aacgaatgag caggatgcag atatcacggt atcaacggca
     1321 caccggagca aggggctgga gtggccggtt gtaatgctgg atgaggattt ccaggatatc
     1381 acggatccgc tgatgacgga ggccgagcgt catgatgaaa cctgtctgtt atatgtggct
     1441 gtcacccggg ccctcaggac gctggtactg aatgacctga tgcaccaact ggcagaagaa
     1501 cagcagaaaa ctgaaggtt
//
LOCUS       DAZFLD010000464         1491 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.467, whole genome shotgun sequence.
ACCESSION   DAZFLD010000464 DAZFLD010000000
VERSION     DAZFLD010000464.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1491
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.467"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>1491
                     /locus_tag="ACHKBL_005062"
     CDS             <1..>1491
                     /locus_tag="ACHKBL_005062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5821774.1"
                     /translation="PDGRTVREKLRHEIIHHAMEHVVTPAEYQTIIKTVLKTRDSDNV
                     TIREAWRKVDASYGKESPEVQAGEFLAHMAEKQPNKFVAAWERVVALVKGVLRRTGLL
                     KPTELNDIRLVRETIRTLGQRVREGYTPREDGAGASSQYSRSGKPDPFKVPEGEGERY
                     REWLGRAMRSKRSSDIQIELGRTPPVLRHLGAPDLPLVISRDTVRKATNGVKHVVPMD
                     VIERLPELMHDPEAVYQSATHQNSLVILFNLRDRNGDPVISAVHLKAIKNGLEINKIA
                     SVYGTERGKKLSNMERQGLTLYRREKLTPDEDLPRGLQLPVVEDSLGSLNKILYPEDI
                     RKGPYYSRTSSLTPEETIASRFVRQMQDKFQVLKAVQENIRKTGGKVDDSNNAYMAEE
                     LFHGKAENDLNVMKERYVQPLAKLLADYKIAQADLDEYLYARHAPERNAHIAKINPKM
                     PDGGSGMTNAEAAEIMQRVRNSGKQAQYDRLAGIIDDMLARRRELIR"
BASE COUNT          399 a          351 c          426 g          315 t
ORIGIN      
        1 cctgacgggc ggaccgttcg cgagaagctg cgtcacgaga tcattcacca tgccatggag
       61 catgttgtca caccagcgga atatcagacg attatcaaaa ccgtgctgaa aacccgcgac
      121 agtgataacg tcaccatccg tgaagcctgg cgtaaggttg atgcttccta tggtaaggaa
      181 tcaccggaag tacaggcggg tgaatttctg gcacatatgg cggagaaaca gccgaataaa
      241 ttcgtggcgg catgggagcg tgttgttgcc ctggtcaaag gggtactgcg tcgtacgggg
      301 ttactgaagc cgacggaact gaacgatatc agacttgttc gcgagaccat ccgtacgtta
      361 ggccagcgtg tgcgggaagg ttacacgccg cgtgaggatg gtgctggcgc atcgtctcag
      421 tactcccgta gcggtaaacc tgatccgttt aaagtaccgg agggtgaggg tgagcgttat
      481 cgtgaatggt tgggaagagc catgcgctcc aaacgttcat ctgatattca gattgagcta
      541 ggacgcacac ctcctgtatt gcgtcatctt ggtgcgccgg acttgccgct ggttatttcc
      601 cgcgacactg tgcgtaaggc caccaatggc gtgaaacatg tggtgccaat ggatgttatc
      661 gagagactac cggaactgat gcatgatcca gaagccgttt atcagtccgc aactcatcaa
      721 aactctcttg ttattctgtt taatctcaga gacagaaatg gtgatccagt aataagtgca
      781 gtgcacttaa aggccattaa aaatgggctg gagataaaca aaatagcttc tgtatatgga
      841 accgaaagag ggaaaaagtt aagcaacatg gagcgccagg gcttaaccct gtatagaaga
      901 gaaaaattaa ccccagatga ggaccttccc cgggggctcc aattgcccgt ggtggaagac
      961 tcactggggt ctttgaataa aatactctat cctgaagata ttcgcaaggg gccgtattac
     1021 tcccgcacca gcagcctgac gccggaagag acaatcgcat cgcgttttgt tcgccagatg
     1081 caggataaat tccaggtgct gaaggctgtg caggagaata tccgtaaaac gggcggcaaa
     1141 gtggacgaca gtaacaacgc ttatatggcg gaagaactct tccatgggaa ggcggaaaac
     1201 gacctgaacg tgatgaagga gcgctatgtt cagccgctgg ctaaattact ggcggactac
     1261 aaaattgcgc aggccgatct ggatgagtac ctctacgccc gtcacgcgcc ggaacgtaac
     1321 gcgcatatcg cgaaaatcaa cccgaaaatg ccggacggcg gttcggggat gaccaacgcg
     1381 gaagcggcgg aaatcatgca gcgtgtacgt aacagtggca aacaggcaca gtatgaccgt
     1441 ctggcaggga ttattgacga tatgctggcc cgtcgccgtg agcttatccg t
//
LOCUS       DAZFLD010000465         1484 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.468, whole genome shotgun sequence.
ACCESSION   DAZFLD010000465 DAZFLD010000000
VERSION     DAZFLD010000465.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1484
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.468"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..189
                     /locus_tag="ACHKBL_005063"
     CDS             <1..189
                     /locus_tag="ACHKBL_005063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5821775.1"
                     /translation="LAEQKAQAWVFQYRGYHRAAAAAMVKDIPAPTAVHTVAALLAAD
                     SLTELGKMIHEPDRTAGP"
     gene            161..703
                     /locus_tag="ACHKBL_005064"
     CDS             161..703
                     /locus_tag="ACHKBL_005064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000083772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5821776.1"
                     /translation="MSQTALLALDGEGIFMQNMVISPSMQFQEKDQSGQTSSTANAEQ
                     GIKAKELRVTGMITFDNERALQRIFQLASATTGDGALKVYRIANATAAAINFREGTFS
                     GQIDAQQQTDRLAWQVSFTLRERRSVPEKRQARATPGSSRKQTPQTPGAKGGTVADET
                     PEKMTWFEEKVLKPVNDALE"
     gene            706..>1484
                     /locus_tag="ACHKBL_005065"
     CDS             706..>1484
                     /locus_tag="ACHKBL_005065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001070133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5821777.1"
                     /translation="MNPTKRLLLGNDTIQLVSCSVMLELNACGRGFITARTEQNYTGR
                     PVRLDIGYGNDLVRWFTGYVERSQPAENGSVRLLIREMAGILDHPFPCSFQHPTMRTV
                     TQWLSETSGLEIILPDNAAYTDRPVPHFTHSGTGYELLASLGRIFSVPDYIWHPLPDG
                     GIMAAAAAQGMFSGRPVTIPHEFSQASTGGHSMTLPMIQTLRPGVEVNGQRLTMVMLE
                     DDNMTITWTPRNKATGAPLQKSPFRRQAEKAFPELASGLHLP"
BASE COUNT          340 a          464 c          419 g          261 t
ORIGIN      
        1 ctggcagaac agaaggcgca ggcctgggta tttcagtacc gggggtatca ccgcgccgca
       61 gcggcggcca tggtgaagga tatcccggca ccgaccgccg tacataccgt tgccgcactc
      121 ctggcggcag actcattaac tgaactgggg aaaatgatcc atgagccaga ccgcactgct
      181 ggcccttgat ggtgagggta ttttcatgca gaacatggtg atttcgccct cgatgcagtt
      241 ccaggaaaag gaccagtccg gccagacatc gagcaccgcg aacgccgagc agggcatcaa
      301 ggccaaagag cttcgcgtaa ccgggatgat cacctttgac aatgagcggg cccttcagcg
      361 cattttccag ctggcctctg ccaccaccgg ggacggtgcc ctgaaagttt accgtatcgc
      421 aaacgccacc gctgccgcca ttaactttcg tgaagggaca ttttccgggc agatagacgc
      481 acagcagcag accgaccgcc tcgcctggca ggtcagcttc acattacgcg aaagacgaag
      541 cgtaccggaa aaacgccagg cacgcgccac ccccggcagc agccgtaaac agacgccgca
      601 gacgcccggc gcaaaaggcg ggaccgtggc cgacgaaaca ccggagaaaa tgacctggtt
      661 cgaagaaaag gtcctcaaac ccgtcaatga tgcgctggag taaccatgaa ccccactaaa
      721 cgtctgttac tgggtaacga caccattcag ctggtcagct gttccgtcat gcttgagctg
      781 aacgcgtgcg gacgcggatt tatcacggcc agaacagagc agaactacac cgggcgccct
      841 gttcgtctcg atatcggata cggaaacgac ctggtgcgct ggtttaccgg atacgttgaa
      901 cgcagccagc ccgctgaaaa cggctcggtg cggcttctga ttcgtgaaat ggcggggatc
      961 cttgatcacc ccttcccctg ttcatttcag cacccgacaa tgcgcacggt cacacagtgg
     1021 ctgagtgaga ccagcgggct ggaaatcatc ctgccggaca acgcggctta cactgacaga
     1081 cccgttccgc atttcacgca cagcggcacg ggctacgaac tgctggccag tctggggcgt
     1141 attttctccg taccggatta catctggcat ccgttaccgg acggcggcat tatggccgct
     1201 gccgccgcgc aggggatgtt ttccggtcgc cccgtcacca tcccgcacga gttcagccag
     1261 gcatcaacgg gcggacacag tatgaccctc ccgatgattc agacgctgcg accgggtgtg
     1321 gaggtgaacg gtcagcgcct gacgatggtc atgctggaag atgacaacat gaccatcacg
     1381 tggacaccac gcaataaagc caccggcgcc cccctccaga agtccccttt ccgtcgccag
     1441 gctgaaaagg ccttccctga actggcatca gggcttcacc tgcc
//
LOCUS       DAZFLD010000466         1478 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.469, whole genome shotgun sequence.
ACCESSION   DAZFLD010000466 DAZFLD010000000
VERSION     DAZFLD010000466.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1478
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.469"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1431
                     /locus_tag="ACHKBL_005066"
     CDS             <1..1431
                     /locus_tag="ACHKBL_005066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000817024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase-like DNA-binding domain-containing
                     protein"
                     /protein_id="HFO5821778.1"
                     /translation="LVIELPVKKKERGKGDDATSLALNQMGASQRGEVLLAHYGGELA
                     INADSDTVHYYNSVVWVPVQDKELQRTMAQIFIDEDICYSQNAIKSAVDTMKLSLPVM
                     GNTACNLIGFSNGVFDTRTGDFREHDKNDWLLIASELPFTPPAEGETLATHAPNFWKW
                     LRRSVAENDRKADRVLAALFMVLANRYDWQLFIEVTGPGGSGKSVMAEICTMLAGKAN
                     TVSASMKALEDARERALVVGFSLIILPDMTRYAGDGAGIKAITGGDKVAIDPKHKAPY
                     STRIPAVVLAVNNSAMSFSDRSGGISRRRVIFNFSEVVPENERDPMLPEKIEGELAVV
                     IRHLLTRFADQDEARRLLYEQQKSEEALAIKREGDSLVDFCGYLMSSVMCDGLLVGNA
                     EIIPFSPRRYLYHAYLSYMRAHGFSKPVTLTRFGADMPGAMAEYGREYMKKRTKEGVR
                     SNVILTDESEDWLPSCAPVKNDGDKN"
BASE COUNT          399 a          311 c          419 g          349 t
ORIGIN      
        1 ctggttattg agctgcctgt aaagaaaaaa gagcggggaa aaggtgatga tgccacatca
       61 ctggcgctaa accagatggg ggcaagccag cgcggcgaag ttcttctggc acattatggc
      121 ggcgaactgg caataaacgc cgactctgac acagtgcatt attacaacag tgttgtatgg
      181 gttcccgtac aggataaaga gctacagcgc acaatggcgc aaatcttcat tgacgaagat
      241 atctgctatt cacaaaacgc cataaaatcg gcggttgata ccatgaagtt aagtttaccc
      301 gtaatgggga ataccgcctg taacctgatt ggattcagta acggggtatt tgatacccgt
      361 acaggtgatt tccgtgaaca cgataaaaat gactggctgt taattgccag tgaattaccg
      421 ttcaccccac cagcagaggg tgaaacgctg gcaacacatg cgccgaattt ctggaagtgg
      481 ttacgccgtt cggtggctga gaatgaccgc aaggcagatc gcgttctggc agcgttattt
      541 atggtgctgg cgaaccggta cgactggcag ttattcattg aggtaacagg tcctggtgga
      601 agcggtaaaa gtgttatggc agagatttgt accatgctgg cgggtaaggc caacacggta
      661 tcggcaagca tgaaggcact ggaagatgca agggaacgcg cgttagtggt tggcttttca
      721 ctgattattc tgccggatat gacccgctac gcgggggatg gggcagggat aaaggccatt
      781 acgggtggtg acaaggtggc aattgacccg aaacacaaag cgccatactc aacgcgtatt
      841 ccggcggttg tgctggcggt taacaacagc gccatgtcat tcagtgaccg cagcgggggg
      901 atctcacgtc gtcgtgtgat attcaatttt tcggaggttg taccggaaaa cgaacgcgat
      961 ccgatgctgc cggaaaaaat agaaggtgag cttgctgtag tgattcgtca tctgcttaca
     1021 cggtttgctg atcaggacga agccagacgc cttttatatg agcagcagaa atcagaagaa
     1081 gcactggcga taaagcgtga gggggattcg ctggtggact tctgcggcta tctcatgtcg
     1141 tcggtaatgt gtgacggcct gttagtcggt aatgctgaga ttattccttt cagtccacgc
     1201 cgttatcttt atcacgccta tctctcgtat atgagggcac acggtttcag taagcccgta
     1261 acgctgacgc gcttcggtgc agatatgcca ggcgcaatgg cagaatatgg cagggagtat
     1321 atgaaaaaga gaactaaaga aggtgtgcga tcaaacgtga tactgactga tgagtctgag
     1381 gactggctac cgtcatgtgc accagtgaaa aatgatggtg ataaaaatta agttatagaa
     1441 aaactgttca ccactgttca ccctgcaata aatgtatt
//
LOCUS       DAZFLD010000467         1453 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.470, whole genome shotgun sequence.
ACCESSION   DAZFLD010000467 DAZFLD010000000
VERSION     DAZFLD010000467.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1453
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.470"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..1001)
                     /locus_tag="ACHKBL_005067"
     CDS             complement(<1..1001)
                     /locus_tag="ACHKBL_005067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system Vgr family protein"
                     /protein_id="HFO5821779.1"
                     /translation="MNRSLTSKAGSVVQQLSGQSRYRVHVHECSHFLDVLRYSAVESL
                     SQPWRYDVVVTCSSADIACDALLLKPASFTFQVPMSDGTPALPIRTVFGVVESFRRIF
                     ASNDETRYALTIVPRIALLNYTKGSEIYLNQSVTEVVEQVLRKHGLEGQDFEFRLSRE
                     YPPRELITRWRETDLEFIRRLLAEVGIYWRYEMDSRLEQDIVIFQDSQQQYEFGITLP
                     LRNQAGMSDSGQESIWDIQTTCNVVSGSVVTRDYNYREALKPQDSAESIFSKEGITTG
                     ETYHYAEPFLTTGDAESPETGAYFARLRHERILNAQYCVSGHSSSPYLTPGQVLETDG
                     "
     gene            complement(998..>1453)
                     /locus_tag="ACHKBL_005068"
     CDS             complement(998..>1453)
                     /locus_tag="ACHKBL_005068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000431644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ATPase TssH"
                     /protein_id="HFO5821780.1"
                     /translation="SDLLMQQLSEKPETTESELHELIRPLLRDHFQPALLARFQTVIY
                     RPLTQSAMRTIVEMKLAQVCERLHCHYGLSTSVDERLYDALTSACLLPDTGARNVESL
                     LNQQLLPVLSRQLLSHMAAKQKPQALTLTWSDEDGIVMVFDAQAEGEPS"
BASE COUNT          322 a          425 c          384 g          322 t
ORIGIN      
        1 ccgtccgttt caagtacctg acccggcgtc agataagggc tggaggaatg cccactgaca
       61 caatactgcg cgttcagaat gcgttcgtga cgcagacggg caaagtatgc cccggtctcc
      121 gggctttcag cgtcacctgt cgtcaggaag ggttcagcat aatggtacgt ttctccggtg
      181 gtgatccctt ccttactgaa aatactttcg gcactgtcct gaggtttcag cgcttcacgg
      241 tagttgtagt cccgggtgac cacacttccg ctcaccacgt tacaggtggt ctgaatatcc
      301 cagatgctct cctgcccgct gtcactcatc ccggcctgat tgcgcaatgg cagcgtgatg
      361 ccaaattcat actgctgctg gctgtcctgg aaaatcacta tgtcctgttc aagacggctg
      421 tccatttcat agcgccagta aatccccacc tccgccagca ggcgccggat aaactcaagg
      481 tcagtttccc gccaccgggt gatgagttcc ctaggtggat actcccggga gagccggaat
      541 tcaaaatcct gcccttccag accatgttta cgtaatacct gctcaaccac ttcagtgact
      601 gactgattga ggtagatttc gctccctttc gtatagttca gaagggcgat acggggaaca
      661 atcgtcagtg cgtaacgggt ctcatcattc gaggcaaaga tacgacgaaa cgactcaacc
      721 acgccgaata ccgtcctgat cggcagggcc ggtgttccgt cagacatcgg gacctggaaa
      781 gtaaatgacg ccggcttcag cagtaacgcg tcacaggcaa tatccgcaga agagcaggtg
      841 acaaccacgt catagcgcca gggctggctg agggactcaa ccgcgctgta acgcaacaca
      901 tccagaaaat ggctgcattc atgcacatga acccggtacc gtgattgccc tgaaagctgc
      961 tgtacaacgc tgccagcctt gctggtcagg gaacggttca tgacggctct ccctctgcct
     1021 gtgcatcaaa taccatcaca ataccgtcct cgtcactcca tgtcagagtc agcgcctgtg
     1081 gtttctgctt cgcggccata tggctcagca actgccggct cagtaccggc agcagttgct
     1141 ggttcagcag actctccaca ttccgggcgc ccgtatccgg cagcaggcag gctgatgtca
     1201 gggcatcgta cagacgttca tcaaccgatg tgctcagccc ataatggcag tgcaggcgtt
     1261 cgcagacctg tgcaagcttc atctccacaa tggtgcgcat ggcagactgc gtcagcggac
     1321 ggtaaatcac ggtctggaaa cgggcaagca gtgctggctg gaagtggtcg cgcagcagtg
     1381 gacggataag ctcatgcagc tccgattccg ttgtctccgg cttttcgctc agttgctgca
     1441 tcagaaggtc act
//
LOCUS       DAZFLD010000468         1453 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.471, whole genome shotgun sequence.
ACCESSION   DAZFLD010000468 DAZFLD010000000
VERSION     DAZFLD010000468.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1453
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.471"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..1001)
                     /locus_tag="ACHKBL_005069"
     CDS             complement(<1..1001)
                     /locus_tag="ACHKBL_005069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system Vgr family protein"
                     /protein_id="HFO5821781.1"
                     /translation="MRSFLTSKAGSVVQQFSGQSRYRVHVHECSHFLDVLRYSAVESL
                     SQPWRYDVVVTCSSADIACDALLLKPASFTFQTPVSDGTPALPLRTVFGVVESFRRIS
                     ASNDETRYALTIVPRIALLNYTKGSEIYLNQSVTEVVEQVLRKHGLEGQDFEFRLSRE
                     YPPRELITRWRETDLEFIRRLLAEVGIYWRYEMDSRLEQDIVIFQDSQQQYESGITLP
                     LRNQAGMSDSGQESIWDIQTTCNVVSGSVVTRDYNYREALKPQDSAESIFSKEGITTG
                     ETYHYAEPFLTAGDAESPETGAYFARLRHERILNAQYLVSGHSSSPYLTPGQVLETDG
                     "
     gene            complement(986..>1453)
                     /locus_tag="ACHKBL_005070"
     CDS             complement(986..>1453)
                     /locus_tag="ACHKBL_005070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000431644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ATPase TssH"
                     /protein_id="HFO5821782.1"
                     /translation="SDLLMQLLDEQPEATEASLHELLRPILRDHFQPALLARFRTVIY
                     RPLTQSAMRTIVEMKLAQVCERLHCHYGLSTSVDERVYDALTSACLLPDTGARNVESL
                     LNQQLLPVLSRQLLSHMAAKQKPQALTLAWSDEDGIVLVFDAQAEGESSCAVS"
BASE COUNT          322 a          432 c          391 g          308 t
ORIGIN      
        1 ccgtccgttt caagtacctg acccggcgtc agataagggc tggaggaatg cccgctgaca
       61 agatactgcg cattcagaat acgttcgtga cgcagacggg caaagtatgc cccggtctcc
      121 gggctttcag cgtcacctgc cgtcaggaag ggttcagcat aatggtacgt ttctccggtg
      181 gtgatccctt ccttactgaa aatactttcg gcactgtcct gaggtttcag cgcttcacgg
      241 tagttgtagt cccgggtgac cacacttccg ctcaccacgt tacaggtggt ctgaatatcc
      301 cagatgctct cctgtccgct gtcactcatc ccggcctgat tgcgcaatgg cagcgtgatg
      361 ccagattcat actgctgctg gctgtcctgg aaaatcacta tgtcctgctc aagacggctg
      421 tccatttcat aacgccagta aatccccacc tccgccagca ggcgccggat aaactcaagg
      481 tcagtttccc gccaccgggt gatgagctcc cggggtggat actcccggga gagccggaac
      541 tcaaaatcct gcccttccag accatgttta cgcaatacct gctcaaccac ttcagtgact
      601 gactgattga ggtagatttc gctccctttc gtatagttca gaagggcaat acggggaacg
      661 atcgtcagtg cgtaacgggt ctcatcattc gaggcagaaa tgcgacgaaa cgactcaacc
      721 acgccgaata ccgttctgag cggcagggcc ggtgttccgt cagacaccgg ggtctggaaa
      781 gtaaatgacg ccggcttcag cagtaacgcg tcacaggcaa tatccgcaga ggagcaggtg
      841 acaaccacgt catagcgcca gggctggctg agggactcaa ccgcgctgta acgcaacaca
      901 tccagaaaat ggctgcattc atgcacatga acccggtacc gtgattgccc tgaaaactgc
      961 tgtacaacgc tgccagcctt gctggtcagg aaactgcgca tgacgactct ccctctgcct
     1021 gtgcatcaaa taccagtaca atgccatcct cgtcactcca tgccagagtc agcgcctgcg
     1081 gtttctgctt cgcggccata tggctcagca actgccggct cagtaccggc agcagctgct
     1141 ggttcagcag actctccaca ttccgggcac ccgtatccgg cagcaggcag gctgatgtca
     1201 gggcatcgta cacacgttca tcaaccgatg tgctcagccc ataatggcag tgcaggcgtt
     1261 cgcagacctg tgcaagcttc atctccacaa tggtgcgcat ggcagactgc gtcagcggac
     1321 ggtaaatcac ggtccggaaa cgggcgagca gtgcaggctg gaagtggtcg cgcagtatcg
     1381 ggcgcagcag ttcgtgcagg ctggcctcag tggcttccgg ttgttcgtcc agcaactgca
     1441 tcagaaggtc act
//
LOCUS       DAZFLD010000469         1443 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.472, whole genome shotgun sequence.
ACCESSION   DAZFLD010000469 DAZFLD010000000
VERSION     DAZFLD010000469.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1443
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.472"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..634)
                     /locus_tag="ACHKBL_005071"
     CDS             complement(<1..634)
                     /locus_tag="ACHKBL_005071"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015572001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008747 - N-acetylneuraminate lyase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HFO5821783.1"
                     /translation="MQCEFKGVISALPTPYDQSQQIDMESLRKLIRFNIEQNIKGLYV
                     GGSTGEAFLQNVAEREKILETVADESDGRLTLIAHVGGISTAESEVLAKAAKKYGYHA
                     ISAVTPFYYPFSFEEHCIHYRKIIDSADGLPMVVYNIPALSGVRFSLDQINELVTIPG
                     VCALKQTSGDLFQMEQIKRNHPELVLYNGYDEIFASGLIAGADGGIGSTYN"
     gene            752..1304
                     /locus_tag="ACHKBL_005072"
                     /pseudo
     CDS             752..1304
                     /locus_tag="ACHKBL_005072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005154742.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
BASE COUNT          432 a          347 c          269 g          395 t
ORIGIN      
        1 tattatatgt actaccgatt cccccatctg cacctgcaat taaccctgat gcaaatattt
       61 catcgtaacc attatataac acaagctctg gatgattcct tttgatttgt tccatctgga
      121 ataaatcacc ggatgtctgt tttaatgcac aaaccccggg tattgtgaca agctcattta
      181 tctgatctaa agaaaaacgc actccgctaa gagccggtat attataaact accatcggaa
      241 gtccatctgc agaatcaata atcttcctgt aatggataca atgctcttca aatgaaaaag
      301 gataataaaa tggagtaaca gcagatatag catgatatcc gtattttttt gcagcttttg
      361 caagtacctc gctttctgcc gtactgattc cgcccacatg cgctataagt gttagtcttc
      421 catctgattc atcggctact gtttcaagaa ttttttctcg ctctgcaaca ttctgaagaa
      481 atgcttctcc agtagagcct ccaacataca atcccttaat attttgttcg atattaaaac
      541 ggatcagttt tcgaagactc tccatatcga tttgctggga ttggtcataa ggagtgggta
      601 atgctgatat aaccccttta aattcacatt gcatttttaa tcaccttcac ttaactgcat
      661 aatgggatac ctagtacacc atcaagtaca aagattatcc aacagaaaag aagcacaatg
      721 tgaagattat caaaaaaaag ccatcagaaa gctgtcatgc cgctcagtta aggatcggtt
      781 gatcgatcca gtgtccagtt actattctct gcgtaaccct ctgcgactat cttgccctgg
      841 aactgattac ccacagccct gaagagtccg acactgtcac gctacacaaa cggcgtcttc
      901 ctctggaaat gatggtctgg tgaattatca gtatggccca tgagcgtaag gaaccttcat
      961 cagattgtga accggctgga catcatgctg ccaggcagta gcgccgtgct ccatgcccgc
     1021 cagcggccag gaagcggagc catacgctgc gtgttcactc aaacagcgca gctctagcat
     1081 aacaccacgc catatccgct ctggtgcgat ctgaatctgc tgaccattga tggcgtgttc
     1141 tgacgaacac aggacacgca agaacacgat aaagcttcac tcgccaaata catagtggaa
     1201 accagacact ttatcctcag gtaaaaatgg tctgtctgat agaactgacc agccatctac
     1261 tgacggcagc ggccttcgat acaatgaatc tggcactaaa taagactggc aatatagccg
     1321 ataatgcgtt gaatcagtgg ctatatcgta tacataaaac agacatcatt attctgtctt
     1381 gttgtccaac acataaaaac taataaaaac ataccattcc ctgtcgtcag agaatttatt
     1441 gat
//
LOCUS       DAZFLD010000470         1436 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.473, whole genome shotgun sequence.
ACCESSION   DAZFLD010000470 DAZFLD010000000
VERSION     DAZFLD010000470.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1436
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.473"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            149..1378
                     /locus_tag="ACHKBL_005073"
     CDS             149..1378
                     /locus_tag="ACHKBL_005073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFO5821784.1"
                     /translation="MSNIWSKEETLWSFALYGTAVGAGTLFLPIQLGSAGAVVLFITA
                     LVAWPLTYWPHKALCQFILSSKTSAGEGITGAVTHYYGKKIGNLITTLYFIAFFVVVL
                     IYAVAITNSLTEQLAKHMVIDLRIRMLVSLGVVLILNLIFLMGRHATIRVMGFLVFPL
                     IAYFLFLSIYLVGSWQPDLLTTQVEFNQNTLHQIWISIPVMVFAFSHTPIISTFAIDR
                     REKYGEHAMDKCKKIMKVAYLIICISVLFFVFSCLLSIPPSYIEAAKEEGVTILSALS
                     MLPNAPAWLSISGIIVAVVAMSKSFLGTYFGVIEGATEVVKTTLQQVGVKKSRAFNRA
                     LSIMLVSLITFIVCCINPNAISMIYAISGPLIAMILFIMPTLSTYLIPALKPWRSIGN
                     LITLIVGILCVSVMFFS"
BASE COUNT          353 a          317 c          309 g          457 t
ORIGIN      
        1 agcaatcaga gacataactg gcacgtaagg tttgcaacca ctaacccacc aatagagagg
       61 tagaaagggc gttaatctcc catacttaac ctggtttatg gtaaattgcc ctccattttg
      121 tttaatttgt agatgatacg ttcagataat gtctaatatt tggtcaaaag aagaaactct
      181 gtggagtttc gcgctctacg gcacagccgt tggtgcaggc acgctcttcc ttcctattca
      241 gttaggttcg gcgggggctg tggtcctgtt tattactgct ctggtcgcct ggcctttaac
      301 atattggcca cataaagcct tatgccagtt catcctctca tcgaaaacat cagcaggtga
      361 agggataacg ggcgcggtaa cacactacta tggcaagaag attggtaatc tgattaccac
      421 gctgtacttc atcgcctttt ttgtcgtcgt gttgatatat gcagtggcaa ttaccaactc
      481 acttacagaa cagctggcaa agcatatggt tattgatctt cgcatccgta tgttggtgag
      541 tctgggggtt gtattaatcc tgaatctcat ttttctgatg ggacgccatg ccactattcg
      601 ggtaatggga tttttggtat tcccattgat tgcctatttc ttatttcttt ctatttacct
      661 ggtcggtagt tggcaacctg atctattaac aacccaggta gagttcaatc agaataccct
      721 tcaccagata tggatatcga ttcccgtgat ggttttcgct tttagccata cgcccattat
      781 ttctacgttt gccatagaca gacgtgaaaa atatggcgaa cacgctatgg ataaatgcaa
      841 aaaaattatg aaagtcgctt atctcatcat ctgcataagt gtattgttct ttgtctttag
      901 ctgcctgctt tctattccac cttcgtatat tgaagcggct aaagaagaag gggttaccat
      961 tttatcggcg ctttctatgc tgccgaacgc cccagcatgg ttgtcaattt ccgggattat
     1021 tgtcgcagta gttgcgatgt cgaaatcatt cctgggtacg tactttggcg ttattgaagg
     1081 tgccacagag gtcgtcaaaa caacattaca gcaggttggt gtaaagaaaa gtcgtgcatt
     1141 taaccgcgca ctatcaatta tgttggtatc gctgattacc ttcattgttt gttgcattaa
     1201 cccgaacgcg atttcgatga tttacgcgat cagcggcccg ctcattgcca tgatactttt
     1261 catcatgcct acgctgtcaa cgtatctcat cccggcgctt aaaccctggc gttccatcgg
     1321 aaatctgatt accctgatcg tgggtatcct gtgcgtatcg gtaatgttct ttagctaata
     1381 tccgttgtgg atggttcaaa attgcttcaa ttttgaacca tcccagtttt cataat
//
LOCUS       DAZFLD010000471         1419 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.474, whole genome shotgun sequence.
ACCESSION   DAZFLD010000471 DAZFLD010000000
VERSION     DAZFLD010000471.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1419
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.474"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(627..1070)
                     /locus_tag="ACHKBL_005074"
     CDS             complement(627..1070)
                     /locus_tag="ACHKBL_005074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5821785.1"
                     /translation="MHHYKFFALIAMLLCGASFASDVYFLCNTEKGTIKLDENKGVLR
                     YTLSKDHKTEFNYESKGSDYSGFKYNHYSRFQTDYFNVSFVNSEYKYTIFSNYEDDRK
                     SSGVSVTSLNSKKESVYYCKSIGIDRLSDLSAKMACDKYSALGCD"
     gene            complement(1057..>1419)
                     /locus_tag="ACHKBL_005075"
     CDS             complement(1057..>1419)
                     /locus_tag="ACHKBL_005075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001026495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramidase domain-containing protein"
                     /protein_id="HFO5821786.1"
                     /translation="EDISNPVAGGYGATSIQYAKLVKAYTLDKKAALKSASWGKFQIL
                     ASNYATAGYASPEDFVFALSKSEKNQLKAFVSFIKADRVLLRSIRTKDWLSFAQRYNG
                     PRQKGYDLKMERNYNASL"
BASE COUNT          423 a          312 c          244 g          440 t
ORIGIN      
        1 ttctctaaat aaattcaatt aattatctaa acaaggtgaa actttcagag gaaatgagcg
       61 cggccccccc taataatctt aagctgggta cagggaagta tcgaccatta cgcagtgtcg
      121 atatcgaatc gtacaaaaaa cagtcttggc gtgggatatt tacacataat tattagctca
      181 caatctccct atacctgtcg tgttttctgg ggattcctca gggagtgacc atagattccg
      241 ttttccaggt gccccccttt ttctccaaaa attgaaaaat aactctgtag atgccgctaa
      301 caatcaattc gagttgatat tttaaagcgt tctgtgcaga aagaaataaa tttcaaaaat
      361 gaatcagggg agaaaagtgc gctttttaac ctgttcagtg tccagaacac atgaaaccga
      421 tcgccattca gtttcatgca tagtcatgca acgcactgaa aatgattctg acgcattttt
      481 ccggtttccc ccgaggaaaa catccctttt tgcgtcagaa tcgcgctcag cgcgttttag
      541 cggcacgtac aattacagga ttataaatcg tcaccagcga ctggcgtaaa aaagcccctc
      601 tctgcaggcg agatttagat gttttattaa tcacacccca aagctgaata tttatcacaa
      661 gccatttttg ccgacaaatc actaaggcga tcaatgccaa tggacttaca ataataaaca
      721 gattcttttt tgctattcag gctcgtaaca ctaactccgc tgctttttct atcatcctca
      781 taattactaa atatcgtgta cttatactct gagtttacaa acgatacatt gaaataatcg
      841 gtttgaaatc gtgagtaatg gttgtattta aaccctgaat aatcactacc tttcgattca
      901 taattaaatt cagttttgtg gtctttcgat aaggtgtacc gaagtacacc tttgttttca
      961 tctagtttga ttgttccttt ttccgtgttg cataaaaaat acacatcact tgcaaacgat
     1021 gccccacaca gaagcattgc aatcaacgca aagaacttat aatgatgcat tgtaatttct
     1081 ctccattttc aaatcatatc ctttctgcct tggtccgtta tatctttgag caaatgaaag
     1141 ccaatcttta gtccgaatag agcgcagtaa tactctatca gccttaatga agctaacgaa
     1201 tgcttttaac tgatttttct cagacttact taatgcaaaa acgaaatcct cagggctcgc
     1261 gtagcctgct gtggcgtagt tcgacgctaa aatctgaaac ttaccccagg atgctgattt
     1321 taaagctgct ttcttgtcta aagtatacgc tttaactagc tttgcatact gtattgatgt
     1381 tgcaccatac ccacccgcaa cagggtttga tatatcttc
//
LOCUS       DAZFLD010000472         1418 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.475, whole genome shotgun sequence.
ACCESSION   DAZFLD010000472 DAZFLD010000000
VERSION     DAZFLD010000472.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1418
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.475"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..772
                     /locus_tag="ACHKBL_005076"
     CDS             <1..772
                     /locus_tag="ACHKBL_005076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001353682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="conjugal transfer protein TraT"
                     /protein_id="HFO5821787.1"
                     /translation="FPLPQPDTVFSGESLLPPPVLSTPGRCPCCRRTVTHRFILEDSW
                     PLQQMADTCRDTVVLLEKNLTRVMRLKKHPVPENADEKKKHTRTLQDAERSLAQARLS
                     ARRLALRHVEKSQIVTTDALSENESELLQPEGPPFHLCAFCHAWHCLNGYAAAQGVMV
                     WLPDLHPASVVALNARALKEIFSDERKRIRQGRAVLNALVQNRLAVEEKFRTWRPADF
                     ADALRRWPPAQRKTLREKMDGVALILMPDSFPDKKYVM"
     gene            827..1186
                     /locus_tag="ACHKBL_005077"
     CDS             827..1186
                     /locus_tag="ACHKBL_005077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5821788.1"
                     /translation="MDTLTAGYQFSADAFRRFIIRTLTDDIPPDGDEKEIARFILRHP
                     AFNGGWLLFLAAEIHERSRTHNSITVTVSVDNYLSWMDIAVSGLPELPDMPFCDFFIR
                     RQQFIYPGVPPTKSGEE"
     gene            1186..>1418
                     /locus_tag="ACHKBL_005078"
     CDS             1186..>1418
                     /locus_tag="ACHKBL_005078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000690882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5821789.1"
                     /translation="MKIHRISPETLITLILAHLAGKADSTAKEEHRLLRRFLRDDDGR
                     LAGILLNIAGILQFNRELSARHNYPATPLTEFS"
BASE COUNT          336 a          421 c          364 g          297 t
ORIGIN      
        1 tttcccgctc ccgcaaccgg acacggtatt cagcggtgaa tccctgctgc caccacccgt
       61 gctctcaaca cccggacgct gtccctgctg tcgcaggacc gtcactcacc gcttcattct
      121 ggaggattca tggccattac agcagatggc tgacacctgc cgggatacgg tcgtcctgct
      181 ggaaaaaaac ctcacccggg tgatgcgcct gaaaaaacat ccggtaccgg aaaacgctga
      241 cgaaaaaaag aaacacaccc gaaccctgca ggatgcggag cggtccctgg cacaggcccg
      301 cctgtcggcc aggcgactgg ccctgcgtca tgtggaaaag tcacaaatcg tcacaacaga
      361 tgcactcagc gaaaatgaaa gtgaactgct gcagcctgaa ggcccgccct ttcatctctg
      421 tgccttctgc cacgcctggc actgcctgaa cggctacgct gccgcacagg gggtgatggt
      481 ctggctccct gatttgcatc cggcctcggt agttgctctc aatgcacggg cactgaagga
      541 aattttcagc gatgagcgta agcgaatcag acagggacgg gccgtactga atgccctggt
      601 acagaaccgc ctggccgttg aggagaaatt tcgcacctgg cgtccggcgg attttgctga
      661 tgcactgcgc cgctggccgc cggcacagcg taaaacactg cgggagaaaa tggacggggt
      721 ggcgctcatt ctgatgccag attcgtttcc tgacaaaaag tacgtcatgt gacacacacc
      781 gctgccggtg acgttctgcc ggccccaact gacagaggaa caaaaaatgg ataccctcac
      841 tgccggatat cagttttccg cagatgcatt ccggcgcttt attatccgga cgctgactga
      901 cgatattccg cctgacggag atgaaaaaga gattgcccgg ttcattctcc gccacccggc
      961 atttaacggc ggctggctgc tctttctggc tgcagaaatt catgaaagat cccgtaccca
     1021 taacagcatc accgtcacag tgagcgtcga taactatctc tcatggatgg atattgccgt
     1081 gtccggtctc ccggaattac cggatatgcc cttctgtgat tttttcattc gccgccagca
     1141 gtttatttat ccgggtgtac ccccgacaaa atcaggagaa gaatgatgaa aattcaccgc
     1201 atatccccgg agacgcttat aacactcatc ctcgcacacc tggccggaaa agctgacagt
     1261 accgccaaag aagagcaccg gctactccgt cggttccttc gggatgatga cggcaggctg
     1321 gccggcatac tgctgaatat tgccggaata cttcagttca acagggaatt aagcgcccgg
     1381 cataattatc cggccacacc cctgactgaa ttttcatt
//
LOCUS       DAZFLD010000473         1417 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.476, whole genome shotgun sequence.
ACCESSION   DAZFLD010000473 DAZFLD010000000
VERSION     DAZFLD010000473.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1417
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.476"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            131..535
                     /gene="stpA"
                     /locus_tag="ACHKBL_005079"
     CDS             131..535
                     /gene="stpA"
                     /locus_tag="ACHKBL_005079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein StpA"
                     /protein_id="HFO5821790.1"
                     /translation="MSVMLQSLNNIRTLRAMAREFSIDVLEEMLEKFRVVTKERREEE
                     EQQQRELAERQEKISTWLELMKADGINPEELLGNSSAAAPRAGKKRQPRPAKYKFIDV
                     NGETKTWTGQGRTPKPIAQALAEGKSLDDFLI"
     gene            complement(582..1106)
                     /gene="ygaP"
                     /locus_tag="ACHKBL_005080"
     CDS             complement(582..1106)
                     /gene="ygaP"
                     /locus_tag="ACHKBL_005080"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YgaP"
                     /protein_id="HFO5821791.1"
                     /translation="MALTTISPHDAQELIARGAKLIDIRDADEYLREHIPEADLAPLS
                     VLEQSGLPAKLRREQIIFHCQAGKRTSNNADKLAAIAAPAEIFLLEDGIDGWKKAGLP
                     VAVNKSQPLPLMRQVQIAAGGLILIGVILGYTVNSGFFLLSGFVGAGLLFAGISGFCG
                     MARLLDKMPWNQRA"
     gene            complement(1116..1415)
                     /locus_tag="ACHKBL_005081"
     CDS             complement(1116..1415)
                     /locus_tag="ACHKBL_005081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFO5821792.1"
                     /translation="MTELAQLQASAEQAAALLKAMSHPKRLLILCMLSGSPGTSAGEL
                     TRITGLSASATSQHLARMRDEGLIDSQRDAQRILYSIKNEAVNAIIATLKNVYCP"
BASE COUNT          386 a          331 c          337 g          363 t
ORIGIN      
        1 gaaaataaca gctgaaataa tctcgtgcag gactgtaaat agattaaatt ttgtggaaat
       61 ataataagtg atcgcttaca ctacgcgacg aaatactttt ttgttttggc gttaaaaggt
      121 tttctttatt atgtccgtaa tgttacaaag tttaaataac attcgcaccc tccgtgcgat
      181 ggctcgcgaa ttctccattg acgttcttga agaaatgctc gaaaaattca gggttgtcac
      241 taaagaaaga cgtgaagaag aagaacaaca gcagcgtgaa ctggcagagc gccaggaaaa
      301 aattagcacc tggctggagc tgatgaaagc tgacggaatt aacccggaag agttattggg
      361 taatagctct gctgcggcac cacgcgctgg taaaaaacgc cagccgcgtc cggcgaaata
      421 taaattcatc gatgttaacg gtgaaactaa aacctggacc ggtcagggcc gtacaccgaa
      481 gccaatcgct caggcgctgg cagaaggtaa atctctcgac gattttctga tctaattact
      541 gaggccggat gttgctgcta tggcgcgtcc ggcgtcgctt ctcaagctcg ttggttccac
      601 ggcatcttat ctaacaacct tgccatccca caaaaaccgc taattcctgc aaacagtaac
      661 ccggcaccaa caaagccgct taataagaag aaaccgctat ttacggtata acctagtata
      721 acgccgatta atattaaacc acccgcagcg atctgcacct ggcgcattaa cggcaagggt
      781 tgagatttat ttaccgctac tggtaatcct gcttttttcc agccatcaat cccatcttcg
      841 agtaaaaaga tttctgcggg ggcggcaatc gctgctaatt tatcggcgtt attactggtg
      901 cgtttaccag cctggcagtg gaatattatt tgctcgcgac gtaatttagc cggaagacct
      961 gactgttcca gcacggataa tggagccaga tctgcttcag gaatatgttc acgaagatat
     1021 tcatcagcgt cacgaatatc gattaacttt gcgccgcgtg cgattaattc ttgtgcatca
     1081 tgcggcgaaa tggttgtcaa agccatagtg actccttacg gacaatagac atttttcagg
     1141 gtggcgataa tggcatttac cgcctcattt ttaatggaat atagaatgcg ttgggcatcc
     1201 cgttggctgt cgataagccc ttcgtcccgc atacgagcga gatgctgtga tgttgcagag
     1261 gcactcagtc cggtaatgcg cgtcagctct cccgcactgg tgccggggga accgctaagc
     1321 atgcacagaa tcagcaaccg tttagggtgg ctcattgctt tcaataaggc cgctgcctgt
     1381 tcggcactag cctgtaattg cgcgagttca gtcatag
//
LOCUS       DAZFLD010000474         1416 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.477, whole genome shotgun sequence.
ACCESSION   DAZFLD010000474 DAZFLD010000000
VERSION     DAZFLD010000474.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1416
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.477"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(77..424)
                     /gene="tnpB"
                     /locus_tag="ACHKBL_005082"
     CDS             complement(77..424)
                     /gene="tnpB"
                     /locus_tag="ACHKBL_005082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019104956.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFO5821793.1"
                     /translation="MIPLPSGTKIWLVAGITDMRNGFNGLAAKVQTALKDDPMSGHVF
                     IFRGRSGSQVKLLWSTGDGLCLLTKRLERGRFAWPSARDGKVFLTQAQLAMLLEGIDW
                     RQPKRLLTSLTML"
     gene            complement(421..801)
                     /locus_tag="ACHKBL_005083"
     CDS             complement(421..801)
                     /locus_tag="ACHKBL_005083"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFO5821794.1"
                     /translation="MQKNVTPGRRKGCPNYPPEFKQQLVAASCEPGISISKLALENGI
                     NANLLFKWRQQWREGKLLLPSSESPQLLPVTLDAAAEQPESLAEDAETLSISCEVTFR
                     HGTLRFNGNVSEKLLTLLIQELKR"
     gene            1095..1415
                     /locus_tag="ACHKBL_005084"
                     /pseudo
     CDS             1095..1415
                     /locus_tag="ACHKBL_005084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997724.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          336 a          376 c          375 g          329 t
ORIGIN      
        1 gatgtcgtca gaagagatgt cgttcataag cccgtatttt accgggctta ttctgtgaca
       61 accaggataa agagatttac agcatggtca gggaggtcag cagccgctta ggctgtcgcc
      121 agtcgatacc ttccagcagc atcgccagct gcgcctgcgt aaggaacact ttgccatcac
      181 gggctgacgg ccaggcgaag cgcccacgct ccagccgttt ggtcaggagg cacagcccgt
      241 caccggtgga ccacagcagt ttaacctgac tgccgctgcg gccccggaaa atgaaaacat
      301 ggccggacat gggatcgtct ttcagcgccg tctgtacttt cgcagccagg ccgttgaagc
      361 catttctcat atcggtgata ccggcaacca gccaaatttt ggtcccggaa ggtaacggga
      421 tcatcgcttc agttcctgta tcagcagagt caggagcttt tcgctgacat tgccattgaa
      481 gcggagcgtc ccgtgccgga acgttacctc acagctgata ctgagggttt ccgcgtcctc
      541 tgcgagcgat tctggctgtt cggcagctgc atcgagagtc acaggaagta gctgggggct
      601 ctctgaagaa ggtaatagca gctttccctc gcgccattgt tgtcgccatt tgaacaacag
      661 attggcgtta atgccatttt caagagcaag ttttgagatg gatatcccgg gttcacagga
      721 ggcagcaacg agctgctgtt taaattcggg aggataatta gggcagcctt ttcgcctgcc
      781 gggagtcaca tttttctgca tatctgacac tttggttccc actacttatt tggtggacac
      841 cactttgtct aattcgtcag attctgacca gacggttcag gctgtgcgct tacgaacaaa
      901 cacggcgcgt tcgtcttcat gaaacaccgg ccgcctggtg ccacacacgc cgggaatgct
      961 acagcaaaac gaccggtcgt cgctgcggca gccctgacag tctcagcagg ctgattctga
     1021 gcagtattca gcccatagaa tgagataacc cgtatgaatg atgacagacg tctacggtac
     1081 ccgatgtatg gaacattttc tttctggcta acggcatgcc cgacacatgg ttttacaccg
     1141 atgacgcctc cgtcacaaaa gagtgggggt gggaatattg tgacaatgtg gaagtgttcc
     1201 ctctgtgcta tatgcatgac ggtcccgttt tccgggggcg ctatattgat gagctcagcg
     1261 ccgcacggag cagaatgctc catatgcacc acatactgcc ggaatgaacc cggtacagag
     1321 aaaattttca tgacgcagaa tgtatccgtg aaattcacga agcgcccctg caggctcccg
     1381 gcattctgca aaaatacaaa cgcacaaaat cataag
//
LOCUS       DAZFLD010000475         1411 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.478, whole genome shotgun sequence.
ACCESSION   DAZFLD010000475 DAZFLD010000000
VERSION     DAZFLD010000475.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1411
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.478"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..312
                     /locus_tag="ACHKBL_005085"
     CDS             <1..312
                     /locus_tag="ACHKBL_005085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head completion/stabilization protein"
                     /protein_id="HFO5821795.1"
                     /translation="LAAVAEVNTTLAPVVAHWKAQGHERAADVPGARLDDETQLVAQY
                     KKAVYARAKADLMGEFATIGRRETYPGQESEDTRATLLAEASLVMRNMLGHRRAGVHI
                     I"
     gene            309..782
                     /locus_tag="ACHKBL_005086"
     CDS             309..782
                     /locus_tag="ACHKBL_005086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO5821796.1"
                     /translation="MNQLESLTAFITENLPSDAMQFFMSSQEDAQLIRSQKALGNDQI
                     RMGVMTYTGVIAWDNFPFRKYSPGLVYALVMAWLEEHADPLREEFCPEDVNVDPQFND
                     EDSCLLDITVGLADPLIIRVQEDGPVPYRGKRWTLVNPEIWTAETMDLFVSRSRE"
     gene            779..>1411
                     /locus_tag="ACHKBL_005087"
     CDS             779..>1411
                     /locus_tag="ACHKBL_005087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HFO5821797.1"
                     /translation="MISGQLNPQQLAEAQAMLQSLDLPPKKRQRLLWRIARHGIIAAA
                     RRHQRNQCAPDGTPWAPRKNKKKKAGKNTGKMLKKLPRLMAVREIPGQDAVKVYFRAV
                     RYGPTASASNVAWAHHNGATIRYHGDSFRHKKPARSVPCSLKQAQALRAWGLELKSGK
                     RWRKATLSMIRRRVSNRQAGWLLARFERDDWEDFRDLYSSDGQGIRQRNNP"
BASE COUNT          346 a          394 c          439 g          232 t
ORIGIN      
        1 cttgccgcag tggccgaggt aaacaccacg ctggcgccgg ttgtggcgca ctggaaggcg
       61 caggggcatg aacgcgccgc agacgtaccg ggggccaggc tggacgatga aacccagctg
      121 gtcgcacagt acaaaaaggc cgtttatgcc cgtgccaagg ccgatcttat gggcgagttc
      181 gccaccattg gtcgccgtga aacctatccg gggcaggaaa gcgaggacac acgcgccacc
      241 ttactggctg aagcctcgct ggtcatgcgt aacatgctgg ggcacagacg cgcgggagtg
      301 cacattatat gaaccagctg gaaagcctga ccgccttcat taccgaaaat ctgccgtctg
      361 acgccatgca gttcttcatg agcagtcagg aagacgcgca gcttatccga agtcagaagg
      421 cactgggtaa cgaccagatc cgcatgggcg tgatgacgta caccggcgtg attgcatggg
      481 ataacttccc gttccgcaaa tactcaccgg ggctggttta cgcgctggtc atggcctggc
      541 tggaagagca cgcggacccg ctgagggagg aattctgccc ggaggatgtg aacgtcgacc
      601 cgcagtttaa cgatgaggac tcgtgccttc tcgatatcac ggtgggactg gctgacccgc
      661 tgatcatccg ggtgcaggaa gacggccccg ttccgtacag ggggaaacgc tggacactgg
      721 ttaaccctga aatctggacg gcggaaacca tggatctgtt tgtcagcagg agccgcgaat
      781 gataagcggc cagctgaacc cgcagcaact ggcagaagcg caggcgatgc ttcagtcgct
      841 ggatctgccc ccgaaaaaac gccagcgact gttatggcgt attgcccggc acggcattat
      901 cgccgcagcc agacgccatc agcgcaacca gtgcgcacca gacggcacgc cgtgggcacc
      961 acggaaaaac aaaaaaaaga aggcaggcaa gaacaccgga aagatgctta aaaaactccc
     1021 gcgccttatg gcagtgcggg agataccggg gcaggacgcg gtgaaggtgt atttccgcgc
     1081 tgtaagatac ggaccgacag cgtcggcgag taacgtcgcg tgggcacatc acaacggtgc
     1141 caccatccgc tatcacggcg acagcttccg gcacaaaaaa ccggcgcgtt ccgtaccgtg
     1201 ttccctgaag caggcacagg cgctgagagc gtgggggctt gagctgaaaa gcggcaagcg
     1261 ctggcgaaaa gccaccctgt caatgatacg gagaagggta tctaaccgac aggctggctg
     1321 gctactggca agatttgagc gcgacgactg ggaagatttt cgcgaccttt acagcagtga
     1381 tggtcaggga atacgacaac gtaacaaccc a
//
LOCUS       DAZFLD010000476         1404 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.479, whole genome shotgun sequence.
ACCESSION   DAZFLD010000476 DAZFLD010000000
VERSION     DAZFLD010000476.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1404
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.479"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..707
                     /locus_tag="ACHKBL_005088"
     CDS             <1..707
                     /locus_tag="ACHKBL_005088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type VI immunity family protein"
                     /protein_id="HFO5821798.1"
                     /translation="LSFALPYARERMFPCEFALAQRFSGVMVDSLGTLEGGGAVIGLK
                     GACWYTILGTPWLEKLGGAERVKHRLAKTPEISLLPYNNGVVLKAGELPPPLGEMKTE
                     GLSPLLVKVNQVIKPVRFNESRSLHFYSEYEGLQFDKESTMKWYRRFDEASALLDKEE
                     MGKSCAPERIKCWTDEIAPHAGQWAAMVNGTTEYIQTREGQKMPPFEDKYGKQHRACW
                     KLLKRDDNGSVYTLPE"
     gene            733..1112
                     /locus_tag="ACHKBL_005089"
                     /pseudo
     CDS             733..1112
                     /locus_tag="ACHKBL_005089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004160259.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2345 domain-containing protein"
     gene            1109..>1404
                     /locus_tag="ACHKBL_005090"
     CDS             1109..>1404
                     /locus_tag="ACHKBL_005090"
                     /inference="COORDINATES: protein motif:HMM:NF024894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4123 domain-containing protein"
                     /protein_id="HFO5821799.1"
                     /translation="MNSVSFSLAAQHLICSTKVTLFALVDGLQYERYFGESLSVQQPA
                     AVPLFDTWPDSRIAFAGPWVMEMNSIMDFRERLCELEAALPSVSWMISSSSLTEL"
BASE COUNT          340 a          314 c          423 g          327 t
ORIGIN      
        1 ggttgtcgtt tgcacttcct tatgcccgtg agcgaatgtt cccctgtgaa ttcgccctgg
       61 cgcagcgttt ttccggtgtg atggtggatt cactgggaac gctggaaggc ggtggggcag
      121 tcattggtct gaaaggtgcc tgctggtaca ccattctcgg tacgccgtgg ctggaaaaac
      181 tggggggggc agagagagta aaacatcgtt tagctaaaac gcctgaaatc agcctgctac
      241 cctataacaa tggtgtcgta cttaaagccg gtgaactccc cccgccactg ggagagatga
      301 aaacagaagg tttatcgccg ctgctggtga aggtgaacca ggtcattaag ccagtgcggt
      361 ttaatgaatc aagatctctt catttctact ctgaatatga aggacttcaa ttcgacaaag
      421 aatccaccat gaagtggtac aggcgctttg acgaagccag cgccctgctc gataaagaag
      481 aaatggggaa atcttgcgcc cccgaacgca ttaaatgctg gaccgatgaa atagcgcccc
      541 atgccggaca atgggccgcg atggttaatg gcaccaccga gtacatccag acccgcgaag
      601 ggcagaaaat gcccccgttt gaggataagt acggtaagca gcacagggct tgctggaaac
      661 tactgaaacg agatgacaat ggtagcgttt atacactacc tgagtaaggt cgtgtgctga
      721 atgaccgcgc aggccggaga gaggctgggg ctttttgccc ggaccggaca actgagcgtc
      781 aagtccgggg aggggccggt agaggttcag gcgaaaaacg gcagcatgcg gttgtttgct
      841 gagaagaagc tgacactgag ctcagcgtgt gatatctctt ttgccgggaa aaaacgtatc
      901 acgctgattg gcgggggagt tacctgcgac ttgaagccgg gaaggtcgag tatggtacga
      961 cggcaaagta tctgcgcaaa gtgaagcgga caatggcggc gggggctgca tcaataccag
     1021 tcaggtccat tgtgggggga ggtatttgcc tgagctgtct gatgaaggcg gcaatgaatg
     1081 gcgacacctt tgttgtaagg ggtgggtcat gaacagcgtc agttttagcc ttgcggcaca
     1141 acacctgatt tgttcaacaa aagtgacatt atttgctctg gtggatggtc tgcaatatga
     1201 gcggtatttc ggtgagagtc tgtctgttca acaacctgcc gccgtgcccc tgttcgatac
     1261 ctggcctgat tcgcgtattg catttgcggg gccatgggtt atggagatga acagcataat
     1321 ggatttcaga gaaaggctgt gtgagcttga agctgcgctt ccgtcagttt cctggatgat
     1381 atcttcttcc tcgcttactg aact
//
LOCUS       DAZFLD010000477         1397 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.480, whole genome shotgun sequence.
ACCESSION   DAZFLD010000477 DAZFLD010000000
VERSION     DAZFLD010000477.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1397
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.480"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(131..1375)
                     /locus_tag="ACHKBL_005091"
     CDS             complement(131..1375)
                     /locus_tag="ACHKBL_005091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000037966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA C-terminal domain-containing protein"
                     /protein_id="HFO5821800.1"
                     /translation="MSKKFTKTLLSSSLVVGLLSVSSFTSASEYSYTALDIVGRHGTT
                     YTIVYNSDSDTAYVKWKTVPAGAEGIESGSIVQGLSKINRDGTVEVTTKDGTSTYRVY
                     DREFHDLMNSLVKQKREGSLGKNEGTKTELVSDADIRNLFGRIAAISEMETNVGLDEY
                     GRVATSAESIKERVSLQSYLATEAANSAIGLDKNGKLTLNETDIVTPLRINDGVVTIA
                     KDVEEQKEGLAIQKKGLSELSSRTDIIDAATAANTNKIDSRTTVGVNADGTLTSADGA
                     TKTIAVNDGLVALSGRTDRIDATVGSVDRRVTKNTQAIQSNTRQLQEHNARLNSQQRQ
                     IRENHEEMKRAAAQSAALAGLFQPYSVGKFNATAALGGYSDKQAVAVGVGYRFNEQTA
                     AKAGVAASDGDVSYNVGVNFEF"
BASE COUNT          353 a          377 c          263 g          404 t
ORIGIN      
        1 ccatactcca gataacggtt gcgggtcgcc agaatcactt tcatcgctgt cacctgtttc
       61 atgctcacat gagaaaaaac ggcaggcaaa actccacgcc tgccgtcatg cttcatgtca
      121 ctgtcagcaa ttaaaactca aagttcacac ctacgttgta ggacacatcg ccatcactcg
      181 ctgccacacc tgctttcgct gctgtctgct cattgaaacg gtaaccaaca cccacggcaa
      241 cagcctgttt atcactgtac ccccccaggg cagcagtggc gttgaatttc cccacactgt
      301 acggctggaa aagccccgcc agcgctgcac tctgtgctgc tgcacgcttc atctcttcgt
      361 gattctcacg aatttgacgc tgctggctgt tcaggcgtgc attatgttcc tgcaactggc
      421 gagtattgga ctgaatagcc tgtgtatttt tagtaacccg gcggtcaacg ctaccaacag
      481 tagcatcaat acggtctgtt ctgccggaca gagcaacgag accgtcatta acagcaatcg
      541 tttttgtagc gccatcagcg ctggtcaagg ttccgtccgc attcacacca acggtggttc
      601 gggaatcaat tttattcgta ttcgcagccg ttgctgcatc aatgatatct gtgcgggatg
      661 aaagctcaga cagtcctttc ttctggatcg ccagcccttc tttctgttct tccacatctt
      721 tcgctatagt gacaacgccg tcattgatac gtaacggcgt tacaatatct gtctcattca
      781 gagttagctt accgttttta tctaggccaa ttgcgctatt tgcagcctct gtagccagat
      841 aactctgcaa agacacgcgt tctttaatac tctctgcact agttgccact cgcccatatt
      901 catccagacc aacattagtt tccatttcag agatcgcagc aattctgcca aaaagattcc
      961 gaatatcagc atcagataca agctctgttt tagtaccctc gttctttcca agagaacctt
     1021 ctcttttctg cttgaccagt gaattcatta ggtcatgaaa ctcccggtca tacacacgat
     1081 atgtactagt gccatccttg gtagtaactt ctacagtccc atctctatta atttttgata
     1141 atccttgtac aatacttcca ctttcaatcc cttcagcacc ggcaggtact gttttccatt
     1201 taacataagc ggtgtcagaa tcactattat aaacaatggt gtaagtagtt ccgtgtctac
     1261 cgacaatatc caaggcagtg tagctatatt cacttgcact tgtaaaagaa ctaacactca
     1321 acagacctac aaccagagaa gatgacagaa gtgtctttgt aaatttttta ctcattatgg
     1381 atatcctgtt aaattat
//
LOCUS       DAZFLD010000478         1392 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.481, whole genome shotgun sequence.
ACCESSION   DAZFLD010000478 DAZFLD010000000
VERSION     DAZFLD010000478.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1392
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.481"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            163..792
                     /locus_tag="ACHKBL_005092"
     CDS             163..792
                     /locus_tag="ACHKBL_005092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5821801.1"
                     /translation="MSGNATNEIILAKINDILVDFNELNNQINNFPEKFSGKIDAINF
                     AIKQTPDALEESLKKLFSAAEELEKTVQKSTDECRVSMRAYAINELDNLKVKISEAIN
                     ESIKDSIDMPLDQAKERIVQINKMLQNSTGVEMSTRVAFGIGGLLGLSFLSLAMCISA
                     GYLYFQERSKAERYHNAYREQQIVIERLPPETQRQFRDELGRYLTRNQK"
     gene            1100..>1392
                     /locus_tag="ACHKBL_005093"
     CDS             1100..>1392
                     /locus_tag="ACHKBL_005093"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobC"
                     /protein_id="HFO5821802.1"
                     /translation="MVTKKKDKINRDVVISLRLTEKEFGDFVDDIKKSQLSRSAFFRK
                     LILEKSSSVVINVNNTAPLVYHFNKAGNNLNQLAYKVNSAFLMGRISESLYVKV"
BASE COUNT          463 a          202 c          323 g          404 t
ORIGIN      
        1 ctgaatgatg acagggattt tagaacatta atgagggaaa ctgatgatcc agatatactt
       61 gaactattgt ctactgaaag agctataaag cgtcttgcta caggtataaa taaagagctg
      121 gatattgcat ttaatcatct cgaaattata taaggtgtga atatgtctgg aaatgcaaca
      181 aatgaaatta ttttggcgaa aataaacgat attcttgtcg attttaatga gttaaacaat
      241 caaataaata actttcctga aaagttttca gggaaaatag atgcaattaa ctttgccata
      301 aaacagacac ctgatgcgct agaggaatca ttaaaaaaat tattttctgc ggcagaagag
      361 ttagaaaaaa cagtgcagaa aagtacagat gaatgtcggg tatcaatgag ggcgtatgct
      421 ataaatgagt tagataatct gaaagtgaaa atatcagaag ctataaatga atccattaaa
      481 gattctattg atatgccgtt agatcaggcg aaagaacgga ttgttcagat aaataagatg
      541 ctacaaaata gtaccggggt agaaatgagt acaagggtag catttggtat tggtggattg
      601 ctggggcttt catttctttc tttagcaatg tgtatatcag cgggatatct gtattttcag
      661 gagcggagca aagcagagcg ttatcataat gcgtaccgtg aacaacagat agtgatagaa
      721 cgtttgccgc cagaaacgca gcgccagttc cgggatgaat tggggcgtta tcttacccgt
      781 aatcagaagt aaataggcgt tagatggacg ttagatggac attagatgga cattagatgg
      841 atcttagttg gccatctttg atttagatgg acgttagatg gatattagat ggacgttaga
      901 tggacgctag atggacatta gttagctgtc agttacaggg ggagattgag ggtaaattct
      961 ggaggcacgt gaccgactgt atacaaaatg ctggcgcatt ttgatacgtc gacgtgccaa
     1021 aggggacgcc cctttgaaac cccgtcaggg gacggacgac ctggcggtgg cattgttaat
     1081 tatttacagg aggtatcctg tggtcactaa aaaaaaggac aaaattaaca gggatgtcgt
     1141 tatatcctta agattgacag agaaagaatt tggcgatttt gttgatgata taaaaaaatc
     1201 tcaactcagt cgttctgcat ttttcagaaa actgattctt gaaaaatcat catctgtcgt
     1261 aattaatgtt aataatacgg ctcctttagt atatcacttt aataaagctg gtaataatct
     1321 taaccagctt gcttataagg ttaactcggc ttttttgatg gggcgtattt ctgaatcctt
     1381 atatgtaaaa gt
//
LOCUS       DAZFLD010000479         1380 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.482, whole genome shotgun sequence.
ACCESSION   DAZFLD010000479 DAZFLD010000000
VERSION     DAZFLD010000479.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1380
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.482"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            134..1345
                     /gene="bluF"
                     /locus_tag="ACHKBL_005094"
     CDS             134..1345
                     /gene="bluF"
                     /locus_tag="ACHKBL_005094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="blue light-responsive regulator BluF"
                     /protein_id="HFO5821803.1"
                     /translation="MLTTLIYRSHIRDDEPVKKIEEMVSIANRRNMQSDVTGILLFNG
                     SHFFQLLEGPEEQVKMIYRAICQDPRHYNIVELMCDYAPARRFGKAGMELFDLRLHER
                     DDVLQAVFDKGTSKFQLTYDDRALQFFRTFVLATEQATYFEIPAEDSWLFIADGSDKE
                     LDSCTLSPNINDHFAFHPIVDPLSRRIIAFEAIVQKNEDSPSAIAVGQRKEGEIYTAD
                     LKSKALAFAMAHALDLGDKMISINLLPMTLVNEPDAVSFLLDEIKANALVPEQIIVEF
                     TESEVISRFDEFAEAVKSLKAAGISVAIDHFGAGFAGLLLLSRFQPDRIKISQELITN
                     VHKSGPRQAIIQAIIKCCTSLEIQVSAMGVTTPEEWMWLESAGIEMFQGDLFAKAKLN
                     GIPSVAWPEKK"
BASE COUNT          392 a          292 c          294 g          402 t
ORIGIN      
        1 ctaactcatc ttcaactttt tccatttttt atcaatgtaa agaaacagtt atgttttggt
       61 attaaaagaa agacaccgga agatatgtct gttaccgtct tactctcgcc tctcccatta
      121 ccctggacag ttaatgctta ccacccttat ttatcgtagc catatacgtg acgacgaacc
      181 tgtcaaaaaa atcgaagaaa tggtttcgat agcaaatcgc aggaacatgc agtctgacgt
      241 aacagggatc ttactgttta atggttctca ttttttccag cttctggaag gtccggaaga
      301 acaggttaaa atgatatatc gggctatatg ccaggatcca cggcactata atattgttga
      361 gctgatgtgc gattacgcgc ctgctcgccg ttttggcaaa gcgggaatgg aattatttga
      421 tttgcgcctg cacgagcgag atgacgtttt acaggccgta ttcgacaaag gcacatcaaa
      481 atttcagcta acttatgatg acagagcgct acaatttttt cgtacttttg tccttgcaac
      541 cgaacaagca acctatttcg agatccctgc cgaagactcc tggcttttta tcgctgacgg
      601 atctgataaa gaacttgatt cctgtaccct ttcaccaaat ataaacgacc actttgcctt
      661 tcatcctatt gtcgatccct tatcgcggcg gataatcgct tttgaagcca ttgtgcaaaa
      721 aaatgaagac agcccgtcag ctatagcggt tgggcagcgt aaagaagggg aaatctatac
      781 agcggatctc aaaagtaagg cgcttgcatt cgcgatggca cacgcacttg acctcggtga
      841 taaaatgatt tcaatcaatc tattacctat gaccctggtt aacgaacctg acgcagtctc
      901 ttttttactt gatgaaataa aggccaatgc tctggtgcct gaacaaatca tcgttgaatt
      961 tactgaaagt gaagtcatat ctcggtttga tgagtttgcc gaagcggtta aatcgctaaa
     1021 ggctgctggt atcagtgtag caattgatca ttttggcgca ggttttgccg gtttgttact
     1081 cctgtcacgc ttccagcctg acagaattaa aatcagtcag gaattgatta ccaatgtcca
     1141 taaaagtggg ccacggcagg caattattca ggcgatcata aaatgctgta catcacttga
     1201 aattcaagtc tctgctatgg gtgtgacaac accagaagag tggatgtggc ttgaatctgc
     1261 aggaattgag atgtttcagg gagatctgtt tgcgaaagct aaattgaatg gtatcccttc
     1321 agttgcgtgg ccagagaaaa aataattttc agcacattat ttcacatgat ttcacatgat
//
LOCUS       DAZFLD010000480         1379 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.483, whole genome shotgun sequence.
ACCESSION   DAZFLD010000480 DAZFLD010000000
VERSION     DAZFLD010000480.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1379
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.483"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..1234)
                     /locus_tag="ACHKBL_005095"
     CDS             complement(<1..1234)
                     /locus_tag="ACHKBL_005095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000778885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO5821804.1"
                     /translation="MKLTINEKIGFGAGDMAIAIVMMSLAMIITYFYTDVFGLKPVDL
                     GILLFSVRILDAVIDPVVGTMTDRTNTRWGKYRPWLLFMSIPFGISIWLMFTTPNTDY
                     SVKLLWAWSTYVLLTLTYTLIAIPYVSLISVITDDPQERLSANGYRFVMTKIAMFAVT
                     IIVPLSAMYLGKNNVQLGYQIAMGAMGILATCLCLYCFFSVRERIYHPKPSLGMAAQF
                     RLLIKNDQWLILGAVIAIIMFGGIIRNSVAAYYAKYYLNGGDALISPFLTSGVIASVL
                     AMIACTWLTRLYDKIKIFRYTQLLAFVVGGAMYFVQPNSIVLAFTFYIVVTFLTDIQL
                     PIYWASIAESVDYGEMKTGQRVSGLAFGGILFFQKFGMGLAGGFIGIALAFMDYQPGV
                     EQTPQALWGICLLMTIIPA"
BASE COUNT          475 a          317 c          248 g          339 t
ORIGIN      
        1 tcgcgggaat gatcgtcatt aacaggcaga tcccccacaa cgcctgtggt gtttgttcta
       61 cgccaggttg atagtccata aacgctaaag cgattccgat aaaccctccc gccagtccca
      121 taccaaactt ttgaaaaaag agaatcccgc caaatgccag tccagaaaca cgttgccccg
      181 ttttcatttc accataatcc actgactcag caatggatgc ccaataaatg ggaagctgaa
      241 tatcagtcag aaaggtcacc acaatataaa atgtaaaagc taacactata gaatttggtt
      301 gcacgaaata catcgcacca cccaccacga acgccagtaa ttgggtataa cgaaatattt
      361 ttattttgtc gtagagtcga gtaagccagg tgcaagcgat catagccaga acagaggcga
      421 tcacgcctga agttaaaaac ggtgaaatta aagcatcccc accattcagg taatattttg
      481 cataatatgc agcgacagag ttacgaataa tcccgccaaa cataatgatc gcaattacag
      541 cgcccaaaat gagccattga tcatttttga tcagtaaccg aaactgcgcg gccataccga
      601 gactgggttt tggatggtag atgcgctctc tcacgctaaa aaaacaatac aggcataaac
      661 acgttgccag aattcccatt gcccccattg ctatttgata acccagttgt acgttattct
      721 tcccaagata cattgcagat aaaggaacaa taattgttac ggcgaacatg gcaatttttg
      781 tcataacaaa acgataacca ttggcgctca atcgctcttg tggatcatcc gtgataacgc
      841 tgattaatga aacataagga atagcgatta acgtataggt caaggtaagt aacacatagg
      901 tagaccaggc ccataaaagc ttaactgaat aatcggtatt aggtgtggtg aacatcaacc
      961 aaatgcttat cccaaacggt attgacataa aaagcaacca cggccgatat tttccccaac
     1021 gtgtattcgt tctgtctgtc attgttccca caactggatc aatcacagca tcaaggatac
     1081 gtacagaaaa taacagaata cctaagtcga cgggtttcag accaaataca tccgtgtaaa
     1141 aataagtgat gatcatggcc aaagacatca ttactatcgc gatagccata tcaccagcac
     1201 caaaacctat cttttcattt attgtaagtt tcatacacag cactcattga ttacgtgaac
     1261 ttatattaaa aacaaatata tctgaactca ttcagcacat aactttacga gtgagcataa
     1321 atgcattggt tgataccaca acaaaccatt gagtcaatat tgagcaacat cacataaaa
//
LOCUS       DAZFLD010000481         1377 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.484, whole genome shotgun sequence.
ACCESSION   DAZFLD010000481 DAZFLD010000000
VERSION     DAZFLD010000481.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1377
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.484"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            15..1169
                     /gene="ompC"
                     /locus_tag="ACHKBL_005096"
     CDS             15..1169
                     /gene="ompC"
                     /locus_tag="ACHKBL_005096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HFO5821805.1"
                     /translation="MKKMTVALSAVAVAVMFAAGAQAAEVYNKDGNKLDLYGRATALH
                     YFSDDKGNDGDQTYARLGFKGETQINDQLTGFGQWEYQFSGNKTESEGSAGNKTRLAF
                     AGLRFADVGTIDYGRNYGIAYDVGSYTDVLPEFGGDGWTQTDNFMTARTSGVLTYRNT
                     DFFGLVDGLNFAAQYQGKNERDDLQKANGDGYGFSASYEFDGFGFVAAYTKSDRTDKQ
                     VQGLNGTADVLDPESGKKVGEKTVDSGSVAKGKHAEFWGTGLKYDANNLYLAAVYSET
                     QNMTTFGDQGVADKAQNIEAVVQYQFDFGLRPSLAYLQSRGQDVMVGGVNHGDQDLVK
                     YIDVGATYYFNKNMSTYVDYKINLIDESEFTRKAGVATDNIVAVGMTYQF"
BASE COUNT          355 a          254 c          359 g          409 t
ORIGIN      
        1 ataaggatta aatcatgaaa aaaatgacag tggcactttc tgctgtagca gtggcagtga
       61 tgtttgctgc gggggcgcag gcagcagaag tttataataa agatggtaat aaactggatc
      121 tttacgggcg tgcaaccgct ctgcattact tctcggatga taaaggtaat gacggagacc
      181 agacttatgc tcgtctcggc tttaaaggcg aaacgcagat taatgatcaa ctgaccggat
      241 ttggtcagtg ggaataccag ttctctggta acaaaacgga atctgaaggt tccgcgggaa
      301 ataaaacccg tctggcattt gcaggtctga gatttgcaga tgttggtacc attgattacg
      361 gacgtaacta tggtattgct tacgatgtcg gatcatatac tgacgtactg cctgagtttg
      421 gtggtgatgg ctggacgcag accgataact ttatgacggc ccgaacttcc ggtgtcctga
      481 cttaccgtaa tacagacttc ttcggactgg ttgatggtct gaattttgcg gcgcagtatc
      541 agggcaaaaa tgagcgtgat gaccttcaga aggctaatgg cgacgggtat ggtttctcgg
      601 ccagctatga gtttgatggt tttggttttg tcgctgcgta taccaagtca gatcgtaccg
      661 ataagcaggt tcaggggttg aacggtactg cagatgttct tgatcctgaa tcaggtaaaa
      721 aggttggaga aaaaacagtt gactcaggta gtgtggcaaa aggtaaacat gctgaattct
      781 ggggaactgg tcttaaatat gatgccaata acctgtatct ggcagcagtg tattctgaaa
      841 cccagaatat gacgaccttt ggtgatcaag gtgttgcgga taaagctcag aatatcgaag
      901 ccgttgttca gtatcagttt gattttggtc tgcgcccgtc ccttgcctac cttcagtccc
      961 gcggacagga tgttatggtt ggtggtgtga accatggcga tcaggatctg gttaaatata
     1021 ttgatgtcgg tgcgacttat tactttaaca agaatatgtc cacctatgtt gattataaaa
     1081 ttaacctgat tgatgaaagt gaatttaccc ggaaagccgg tgttgcgaca gataatatcg
     1141 ttgctgtggg tatgacttat cagttctgat tattgctgat aagttaataa atacgggccg
     1201 tctgccctta caggcggcct gtatcaatga aaacacagtt ttcattggtc actgcgatca
     1261 gcaattgcca tctggcatgt gctgatttaa ctttctgtta ttacctttat tggttttatt
     1321 ttaaattgga cttttattgt tcggggcgcg tctgcgcccc ttttttatgg gcggata
//
LOCUS       DAZFLD010000482         1372 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.485, whole genome shotgun sequence.
ACCESSION   DAZFLD010000482 DAZFLD010000000
VERSION     DAZFLD010000482.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1372
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.485"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>1372
                     /locus_tag="ACHKBL_005097"
     CDS             <1..>1372
                     /locus_tag="ACHKBL_005097"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5821806.1"
                     /translation="GKYEINLNGWDIKDADGAEVKIVDSAGNVKSGNLYNIARLYVDM
                     NTNKAITLDSAYNIIGSQKEGTDPAKALIMSDDNDWVYIGGGISPHIGDQNPGSDNNI
                     DMAGGDDILSSVGAVLDGANINMGDGDDKLYTQDGFASSGTRNIIMGDGNDVISVDNS
                     FGGKNTISLGEGNNLFIVGNYVNSIAENDITAGSGDDRIEIGTNLDGKNKIDLGDGDN
                     TIQVGGYITNSHTITGNSGDDIIYVATNIDGSGSFNLGEGNNNFIVGGYIQGKNTIEM
                     GSGDDTVSVSTRIADNVKIQLNAGDDSVYAGGLLNKAIVDLGDGDDVVTLSGISDNGK
                     RNNMEELVSTNAMLTGGEGNDTLKINGSFKLLNMKNISGFETIDLGESSENHLDVGIK
                     SDMLDISSSSGVKIFTIMGGAGNTVDLGKANITSHNSVEQGNYADSWYKGDTVDGYTT
                     YTPVGDK"
BASE COUNT          441 a          182 c          342 g          407 t
ORIGIN      
        1 ggaaagtatg aaataaatct aaatggctgg gacatcaagg atgcagatgg cgctgaagta
       61 aaaattgttg attctgctgg taacgttaaa tcaggaaact tatataatat cgcgcgtttg
      121 tatgtggata tgaatacaaa caaagctata acgctggatt cggcttataa tataattggc
      181 tctcagaaag aaggaaccga tcctgctaaa gctcttatca tgtcagatga taatgactgg
      241 gtgtatattg gtggagggat atctccccat ataggggatc agaatccagg ctctgacaac
      301 aacattgata tggctggcgg tgatgatatt ctgtcatctg ttggtgcagt tttggatgga
      361 gccaatatta atatgggtga tggagatgat aaactatata cgcaggatgg tttcgcttct
      421 tcaggtaccc gaaatattat tatgggagac gggaatgacg ttatttctgt tgataattca
      481 tttggtggaa aaaataccat ttctttagga gagggaaaca acttatttat tgttggtaac
      541 tatgtaaata gtattgctga aaatgatatt acggctggta gtggtgatga cagaatagaa
      601 attggtacaa atctggatgg taaaaacaaa attgatcttg gggatggtga caacactatt
      661 caggttggag gatacataac caactcacat accattactg ggaatagtgg tgatgatatt
      721 atctatgtcg ctacaaatat agatggttct ggctcattta atttaggtga aggtaataac
      781 aactttatag tcggaggata tatccagggg aaaaacacaa ttgagatggg ttccggtgat
      841 gatacggttt ctgtttccac tcgtattgca gataatgtta aaatccaact taatgccggg
      901 gatgacagtg tgtatgcagg tggattgtta aacaaggcaa ttgttgacct cggggatggg
      961 gatgatgtcg tcacattgag tggaatatca gataatggta agcgtaataa tatggaggag
     1021 ttagtatcca ctaatgcaat gcttacagga ggggaaggaa acgatactct gaagattaat
     1081 ggttctttca aattgttaaa tatgaaaaat atatctgggt ttgaaactat tgatcttggg
     1141 gaaagttcag agaatcatct ggatgtcggt ataaaatcgg atatgctgga tatttcatcg
     1201 tcatctgggg tgaaaatttt tactatcatg ggtggggcgg gaaatacagt tgatttaggt
     1261 aaggcaaata taacgtctca caattcagta gaacaaggaa attatgctga ttcatggtat
     1321 aaaggagata ctgtggacgg atacacaacg tatactcctg taggtgataa at
//
LOCUS       DAZFLD010000483         1369 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.486, whole genome shotgun sequence.
ACCESSION   DAZFLD010000483 DAZFLD010000000
VERSION     DAZFLD010000483.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1369
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.486"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..231
                     /locus_tag="ACHKBL_005098"
     CDS             <1..231
                     /locus_tag="ACHKBL_005098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraU family protein"
                     /protein_id="HFO5821807.1"
                     /translation="KAGKKDGYWPPDPVEENTGTRNHKWQRLTPSVSTSCAVFPDGEH
                     EAAETGNAAFALWQPYSCCKKRGQTFLGSTNF"
     gene            245..>1369
                     /locus_tag="ACHKBL_005099"
     CDS             245..>1369
                     /locus_tag="ACHKBL_005099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001506259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrating conjugative element protein"
                     /protein_id="HFO5821808.1"
                     /translation="MKQKMTIPVLAVVLCAGTVQADGNFLSLPKVNNSAFGYGKSVDG
                     AVSDKLFYTLGGGSVISQPATRSNMQKLGVDLGWSSDLMCGNFDLKTTVGNQLNGITD
                     GFKNLMGDVIQGASGAVASLPAMVIQRANPGLYDMLTNGVLQANVAFDKAQLNCQNMA
                     KRMMEFTDSNKWTQAAMLEEYKKIVNTGDGDAIKGDEAGRQVTGEEGQRWIGGQQRGG
                     AGQSAIRPTHDLTAAGFNMMNSLPSTSTSSVSPGNCNGSACMKYRNAEEAARAVVKVL
                     GDKSMRTCGDAKACTSGDADQQPGTSIAGTGFAPMLEEATRVNTELLAGMVNGRDKPT
                     AGNLAKLKAGSLSVTAGVIRALQRDPDNGALTSRLAGELAM"
BASE COUNT          340 a          302 c          462 g          265 t
ORIGIN      
        1 aaagcaggga agaaggacgg ttactggcca ccggatccgg tggaggaaaa tacgggcacc
       61 cggaaccata aatggcagag actgacgcct tcagtctcca cgtcctgcgc agtcttccct
      121 gacggagagc atgaagcggc tgaaacaggt aatgctgcct ttgccctgtg gcagccctac
      181 agttgctgca agaaacgggg acagacattc ctgggcagta cgaattttta agtaagggga
      241 agtgatgaaa cagaaaatga cgatacctgt tctggctgtg gtgctttgtg cgggtacggt
      301 acaggctgac ggaaactttt tgtccctgcc gaaagtgaat aacagtgcgt ttggctacgg
      361 taaatccgtg gacggcgcag tatcagacaa actgttttat accctgggag ggggctcggt
      421 gatatcgcag ccggcaacgc gcagcaatat gcagaaactt ggggtggatc tcgggtggag
      481 cagtgatctg atgtgtggca attttgacct gaaaacaaca gtgggtaacc agctgaacgg
      541 gatcactgat ggtttcaaaa acctgatggg ggatgtgatt cagggggcga gcggagccgt
      601 ggcgagtctg ccggcaatgg tgatacagcg ggctaatccg gggctgtatg acatgctgac
      661 caacggtgta ctgcaggcga atgtggcatt cgacaaagcg cagctcaatt gtcagaacat
      721 ggcgaaacgc atgatggagt tcacggacag caataagtgg acgcaggcgg ccatgctcga
      781 ggagtacaaa aaaattgtga atacggggga cggtgatgcg atcaaagggg atgaagcagg
      841 tcgtcaggtt accggggagg aggggcaacg ctggatcgga gggcagcagc gtggtggagc
      901 gggacagtct gctatccggc cgacacatga tctgacagct gccggtttca atatgatgaa
      961 cagtctgcct tcgaccagta catccagtgt cagtcccggg aactgtaacg ggagtgcctg
     1021 catgaaatac cgtaatgcag aggaagctgc ccgggcggtg gtgaaggttc tgggggataa
     1081 atccatgcgg acctgtggtg atgcaaaggc atgtaccagc ggggatgctg atcagcagcc
     1141 ggggaccagt attgccggca cggggtttgc gccgatgctt gaagaagcga cgcgggttaa
     1201 cacggaactg ctggcaggta tggtcaatgg tcgtgacaag ccgacagccg ggaatctggc
     1261 aaaactgaaa gccgggagtc tgtctgtgac agcgggagtg atccgtgccc tgcagcgtga
     1321 tccggacaat ggtgccctga cgtcgcgtct ggcgggagaa ctggccatg
//
LOCUS       DAZFLD010000484         1357 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.487, whole genome shotgun sequence.
ACCESSION   DAZFLD010000484 DAZFLD010000000
VERSION     DAZFLD010000484.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1357
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.487"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(166..1347)
                     /gene="yqeF"
                     /locus_tag="ACHKBL_005100"
     CDS             complement(166..1347)
                     /gene="yqeF"
                     /locus_tag="ACHKBL_005100"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HFO5821809.1"
                     /translation="MKDVVIVGALRTPIGCFRGALAGHSAVELGSLVVKALIERTGVP
                     AYAVDEVILGQVLTAGAGQNPARQSAIKGGLPNSVSAITINDVCGSGLKALHLATQAI
                     QCGEADIVIAGGQENMSRAPHVLTDSRTGAQLGNSQLVDSLVHDGLWDAFNDYHIGVT
                     AENLAREYGISRQLQDAYALSSQQKARAAIDAGRFKDEIVPVMTQSNGQTLVVDTDEQ
                     PRTDASAEGLARLNPSFDSLGSVTAGNASSINDGAAAVMMMSEAKARALNLPVLARIR
                     AFASVGVDPALMGIAPVYATRRCLERVGWQLADVDLIEANEAFAAQALSVGKMLEWDE
                     RRVNVNGGAIALGHPIGASGCRILVSLVHEMVKRNARKGLATLCIGGGQGVALTIERD
                     E"
BASE COUNT          350 a          389 c          317 g          301 t
ORIGIN      
        1 gtttcatttt taaggtaagg actgaaaaaa gtgcagtgtc gatcacgaat atgtcatgag
       61 aatggaaaaa tcagggagcc ctgaaaaggt atgtcgactc gccagccacc attattcaac
      121 agattaatga cggctgatat ttatcggtaa ggaggaaatg cagagctatt cgtcacgttc
      181 aatggtcaat gccacgccct ggcccccgcc gatacaaagc gtagccagtc ctttgcgggc
      241 attacgtttc accatttcat gaaccagaga aacgaggatt cggcaaccgg aagcgcctat
      301 tgggtgaccg agtgcgatcg cgccaccatt gacattgaca cgacgctcat cccattcaag
      361 catcttgcca accgaaagcg cctgtgcagc aaacgcttca ttagcctcga taagatcgac
      421 atcagccaac tgccagccta cacgctccag gcaacggcgg gtcgcataca ccggcgcaat
      481 tcccatcaat gccggatcta caccaacgct ggcaaatgcg cggatgcggg ccagcacggg
      541 taaattcaac gctcgtgctt tggcttcgct catcatcatt actgcagctg cgccatcgtt
      601 tatggatgat gcattacccg ctgtcacaga accgagacta tcaaatgaag gatttaaacg
      661 ggctaagcct tctgcactgg cgtcagtgcg tggctgctca tcggtatcaa caaccaacgt
      721 ctgaccgtta ctttgggtca ttaccgggac gatctcatct ttaaatcgtc cagcgtcaat
      781 cgccgctcgc gctttttgtt gcgagctaag tgcgtaagca tcctgcaact gacggctgat
      841 gccatattcg cgagccagat tttcggcggt gacaccaata tgataatcat tgaaggcatc
      901 ccacaaccca tcgtgcacaa gactatcaac caactggcta ttgccaagct gtgcaccggt
      961 gcggctatca gtcagaacat gtggtgcgcg gctcatgttt tcctggccac cggcgatgac
     1021 aatatcagcc tcgccacact gtatcgcctg agtagccaga tgcagtgctt taagcccgga
     1081 accgcaaacg tcattaatag tgattgcaga aacgctatta ggaagaccac ctttaatagc
     1141 cgattgcctt gccggattct gccctgcccc tgcagtcaac acctgaccaa gaattacttc
     1201 atccaccgca tatgcaggaa cgccggtacg ttctattaac gctttcacga ccaggctgcc
     1261 aagttccacg gcggaatgac ccgctaacgc accacggaag cagccgatgg gtgtccgtaa
     1321 cgccccgaca atcacaacgt ctttcatcac aacctcg
//
LOCUS       DAZFLD010000485         1353 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.488, whole genome shotgun sequence.
ACCESSION   DAZFLD010000485 DAZFLD010000000
VERSION     DAZFLD010000485.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1353
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.488"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            91..1296
                     /locus_tag="ACHKBL_005101"
     CDS             91..1296
                     /locus_tag="ACHKBL_005101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO5821810.1"
                     /translation="MALTDIKVKTAKPKEKPYKLADGGGMYLLINTNGSKYWRMKYRF
                     AGKEKMLSIGVYPDVTLADAREKRSEARKILAAGGDPGVAKKEEKIAQQMSLKNTFEA
                     IAREWHQSKADRWSLRYRDEIIDTFKKDIFPYIGKRPIAEIKPMELLEALRKMEKRGA
                     LEKMRKVRQRCGEVFRYAIVTGRADYNPAPDLACALATPKKVHFPFLTANELPHFLND
                     LAGYTGSIITKTATQIIMLTGVRTQELRFARWEDIDFKTKLWEIPAEVMKMKRPHIVP
                     LSEQVIALFKQLEPITKHHPLVFIGRNDPRKPISKESINQVIELLGYKGRLTGHGFRH
                     TMSTILHEQGFNSAWIETQLAHVDKNAIRGTYNHALYLDGRREMMQWYADYIDSLSSH
                     ESQCQGPLY"
BASE COUNT          410 a          301 c          304 g          338 t
ORIGIN      
        1 gttatacaag gggtgcattg ggggtatcag gttaattact ggcagaaatc cccctcagga
       61 acatgaattc attaaaagcc ggaggatgtc atggcattaa ccgatattaa agtgaaaacc
      121 gcaaaaccaa aagaaaagcc ctataaactt gctgatggcg gcggtatgta tctactgatt
      181 aacactaatg gttctaaata ctggcgtatg aagtaccgct ttgctggtaa agaaaagatg
      241 ctttctattg gcgtataccc cgatgtaacg ctagccgacg ctcgtgaaaa acgcagcgaa
      301 gccagaaaaa tcctcgctgc tggaggcgat ccaggggtgg caaaaaaaga agaaaaaatc
      361 gcgcagcaaa tgagtttgaa aaatacgttc gaggccattg ctcgcgaatg gcatcagtca
      421 aaagccgatc ggtggtcatt acgctatcgt gatgaaatca ttgatacctt taaaaaagac
      481 atttttccct acattggcaa acgccctatc gccgaaatca aaccaatgga gttactggaa
      541 gcactgcgta aaatggaaaa acgtggagct ctggagaaaa tgcgcaaagt ccgccagcgc
      601 tgtggtgagg tgttccgcta tgcaattgtt actggtcggg cagactacaa ccctgctccc
      661 gatcttgcct gtgctttagc tacaccgaaa aaagtacatt ttccctttct tactgctaat
      721 gaacttcccc acttcctcaa cgatctggcg ggttataccg gaagtatcat cactaaaaca
      781 gctactcaga tcattatgtt aaccggtgta cgaacacaag aattacgttt tgcacgctgg
      841 gaggatatcg attttaagac aaaattatgg gagattccgg cagaagtaat gaagatgaag
      901 cgacctcata ttgtgccgct ctctgagcag gtcattgccc tatttaagca acttgaaccc
      961 atcacaaaac atcatcctct ggtctttatc ggtaggaacg atcctcgcaa gccaattagt
     1021 aaggaaagca ttaaccaagt cattgaatta ctgggatata agggaagact cacagggcac
     1081 ggtttcagac acaccatgag taccattcta cacgaacagg gctttaattc cgcttggatt
     1141 gaaacgcagt tagctcatgt agataaaaac gctatcaggg ggacctataa tcatgccctg
     1201 tatctagatg gtcgccgtga aatgatgcaa tggtacgctg attacattga ttcgctttcc
     1261 agccacgaga gtcagtgtca aggccccttg tactgatatt ttctctcaat tcacaaaatt
     1321 cctccggcct ccaggtctgg aggaattttg ttt
//
LOCUS       DAZFLD010000486         1352 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.489, whole genome shotgun sequence.
ACCESSION   DAZFLD010000486 DAZFLD010000000
VERSION     DAZFLD010000486.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1352
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.489"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>1352
                     /locus_tag="ACHKBL_005102"
     CDS             <1..>1352
                     /locus_tag="ACHKBL_005102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000431134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFO5821811.1"
                     /translation="NVTFIGRPVGADTAGIPSGDLPPVLQRALDKFTRDITASAREGK
                     IDPVSGRDTEIRQMVDILSRRRKNNPILVGDPGVGKTALVEGLALRIAEGNVPESLRP
                     VTLRTLDLGLLQAGAGVKGEFEQRLKNVIDAVQQSPAPVLLFIDEAHTLIGAGNQAGG
                     ADAANLLKPALARGELRTIAATTWSEYKQYLERDAALERRFQMVRVDEPDDETACLML
                     RSLKSRYAEHHNVHITDEAVRAAVTLSRRYLTERQLPDKAVDLLDTAAARVRMSLDTV
                     PEQLTRIRSQLASLGMEKQALLEDIAVGHQNHGERLSAIEQEENVLMTARDDLEQQYA
                     RECELTGELLESRSDISRQSETLHLQQALHDIQQNQPLLSVDVDVRTVAGVVADWTGV
                     PLSSLMKDEQTELLHLEKDIGRRVVGQDVALESIAQRLRAAKTGLTSGNGPQGVFLLV
                     G"
BASE COUNT          284 a          371 c          426 g          271 t
ORIGIN      
        1 aatgtgacct ttatcgggcg tcccgtcggt gcggatacgg cgggtatacc gtcaggtgac
       61 ctgccgcctg tgttacagcg tgctctggat aaattcaccc gggacatcac ggccagcgcg
      121 agagagggga aaattgaccc ggtatcggga cgcgatacgg aaatccgtca gatggtggat
      181 attctctccc gccgccgcaa gaacaacccg attctggtgg gggaccccgg tgtggggaaa
      241 acggctctgg tggaagggct cgccctgcgt attgcggaag gtaacgtacc ggaatcactc
      301 agacccgtca ccctgcgcac ccttgacctc ggcctgctgc aggccggtgc cggcgtgaag
      361 ggtgaatttg aacagcgcct gaaaaacgtg attgatgccg tgcagcagtc accggctcct
      421 gtactgttgt ttatagatga agcccatacc cttatcgggg ccggtaatca ggccggcggc
      481 gcggatgcgg ccaacctgct gaagcctgcg ctggcgcggg gtgaactgcg taccattgcc
      541 gccaccacct ggtctgagta caaacaatac ctggaacgtg acgcggctct ggagcggcgt
      601 tttcagatgg tcagagtgga cgagccggat gatgagacgg cctgtctgat gctgcgctcc
      661 ctgaaatccc gttatgcgga acatcataac gtgcatatca ccgatgaggc ggtacgtgct
      721 gccgtcacac tgtcgcgccg ttatctgaca gaacgccagt taccggacaa ggccgttgac
      781 ctgctcgata ctgctgctgc ccgtgtgcgt atgagcctcg acacggtgcc ggaacagctg
      841 acccgtatcc gttcacagct cgcttccctc ggtatggaaa aacaggcact gctggaagat
      901 attgccgttg gtcatcagaa tcacggcgaa cgtctgtctg ccattgagca ggaagagaat
      961 gtgctgatga cagcacgtga tgacctggag cagcagtacg cccgggagtg cgaactcacc
     1021 ggtgagttac ttgaaagtcg cagcgatatt tcccgtcaga gtgagacact gcatctgcag
     1081 caggcactgc acgacattca gcagaatcag cccctgctca gtgtggatgt tgacgtacgc
     1141 accgttgccg gtgtggtcgc ggactggacc ggggtgccgt tatcttccct gatgaaggac
     1201 gaacagacgg aactgctgca tctggaaaag gatatcggca gacgggtggt cggacaggac
     1261 gtggcactgg aatccattgc gcagcgtctg cgtgcggcga aaacgggtct gacatccggt
     1321 aacggccccc agggggtgtt cctgctggtt gg
//
LOCUS       DAZFLD010000487         1350 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.490, whole genome shotgun sequence.
ACCESSION   DAZFLD010000487 DAZFLD010000000
VERSION     DAZFLD010000487.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1350
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.490"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..954
                     /locus_tag="ACHKBL_005103"
     CDS             <1..954
                     /locus_tag="ACHKBL_005103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000990138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HFO5821812.1"
                     /translation="FWYVIALAGQSNGMAYGEGIPLPDTLDKPESRVKQLARRKTITP
                     GGKECKFNEIIPADHCLHDVQDMSGYHHPAADLHKGEYGCVGQGLHIAKKLLPYIPEQ
                     AGILLVPCCRGGAAFTVGAEGMYVPDTGATADAMRWGTGTALYEDLVARVKVALEYNR
                     KNKLLSVCWMQGEFDLMSPDYEKHPDLFYQMVTSFRSELSEYSSQCVGNSSERVPWLC
                     GDTTWYWKESYQKEYDFIYGHYRQRTDDEIHFLSFQDSNRHELTNEPEEDADDLSVGY
                     LGSSWRTELSWTTSQRSTHFNSMARRGVIAECYAQKIRNYL"
     gene            complement(1269..>1350)
                     /locus_tag="ACHKBL_005104"
     CDS             complement(1269..>1350)
                     /locus_tag="ACHKBL_005104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_062863141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="subtilase cytotoxin subunit B"
                     /protein_id="HFO5821813.1"
                     /translation="LTSNALVGLSTCATSTECFGPDRKKN"
BASE COUNT          387 a          263 c          336 g          364 t
ORIGIN      
        1 ttctggtatg tgatagccct cgcaggacag tctaatggta tggcctacgg tgaaggtatt
       61 cccctgccag atacactcga caaacccgag tcccgtgtaa aacaactggc tcgtcgaaaa
      121 acaatcacac caggaggaaa agagtgtaaa ttcaatgaga ttattccggc agatcactgt
      181 ctgcatgatg ttcaggatat gagtggatat caccatccgg ctgctgattt gcataaaggg
      241 gaatatggtt gtgtcggaca gggattgcat atagctaaaa agttactgcc atacatacct
      301 gagcaagcag ggatattact ggttccttgt tgcagaggag gtgccgcctt tactgttgga
      361 gctgaaggta tgtatgtacc tgatacaggg gcaacagctg atgcaatgcg gtggggcacc
      421 ggaacagcac tctatgagga cttagtagcc cgggttaagg tagctctgga atataacaga
      481 aagaataaac tgctatctgt ctgctggatg cagggggagt ttgatcttat gtcaccagat
      541 tatgaaaagc atcctgactt gttttatcag atggtaactt ctttcaggtc tgagttatct
      601 gaatactcat ctcagtgtgt tggtaattcg tcagaaagag ttccatggtt gtgtggtgat
      661 acaacctggt actggaagga atcttatcaa aaagaatatg attttatata tggacattac
      721 agacaacgga ctgatgatga aatacatttt ctcagctttc aggatagtaa ccgacatgaa
      781 ttaactaatg agccagaaga agatgccgat gatttatctg tcgggtatct ggggtcatca
      841 tggaggacag aattaagctg gacaacgtca cagagaagta cacactttaa ttcaatggcc
      901 agaaggggag ttattgctga atgttatgcg caaaaaataa gaaattattt gtagacaagt
      961 tcaggatata tctacagctc tgacatgatt ctctgagaga catgacggag tggtatggag
     1021 ttgtcggtat caacaccaag atagctggtc gttaccgttt gatatcactg gttctgatac
     1081 tatgtggacg gtagtgtgtc acgaaggcac gcaccaggga cgtgagtccc tgtaatatca
     1141 tcgacagtcc cattatggca tgtctgcacc tacaacgtca tttcgaggtc ggctcagtgt
     1201 tcactcgagt cttatatcac tgacgccgac actccatcca gagtatctct ggacggaaac
     1261 atcacctatt agttcttttt tctgtcaggg ccaaaacatt cagtcgatgt ggcgcaggtc
     1321 gataacccaa caagggcatt ggacgttaat
//
LOCUS       DAZFLD010000488         1348 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.491, whole genome shotgun sequence.
ACCESSION   DAZFLD010000488 DAZFLD010000000
VERSION     DAZFLD010000488.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1348
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.491"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..326)
                     /locus_tag="ACHKBL_005105"
     CDS             complement(<1..326)
                     /locus_tag="ACHKBL_005105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 family protein"
                     /protein_id="HFO5821814.1"
                     /translation="MDQVVVFQKMFEQVRKEQNFSWFYSELKHHRVAHYIYYLATDNI
                     RIITHDDTVLLLRGTRNLLKVSTKKNPAKIKEAALLHISGKSTFREYCSTLADAGVFR
                     WVTDVN"
     gene            complement(547..1278)
                     /gene="bluR"
                     /locus_tag="ACHKBL_005106"
     CDS             complement(547..1278)
                     /gene="bluR"
                     /locus_tag="ACHKBL_005106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor BluR"
                     /protein_id="HFO5821815.1"
                     /translation="MAYYSIGDVAERCGINPVTLRAWQRRYGLLKPQRSEGGHRLFDE
                     EDIQRIEEIKRWISNGVPVGKVKALLETTSQDTEDDWSRLQEEMMSILRMANPAKLRA
                     RIISLGREYPVDQLINHVYLPVRQRLVLDHNTSRIMSSMFDGALIEYAAASLFEMRRK
                     PGKEAILMAWNVEERARLWLEAWRLSLSGWHISVLADPIESPRPELFPTQTLIVWTGM
                     APTRRQNELLQHWGEQGYKVIFHAP"
BASE COUNT          350 a          314 c          299 g          385 t
ORIGIN      
        1 tgattaacat cagtaaccca ccggaaaacg cccgcgtctg ccagtgttga acagtactcc
       61 cgaaatgtag attttccgga aatatgaagc aatgcggcct cttttatttt agcagggttc
      121 tttttcgtac taacttttaa caggttcctg gttcctctta ataacaaaac cgtgtcatcg
      181 tgagtaataa ttctgatgtt atccgtagcc agataataaa tgtaatgtgc aacgcggtga
      241 tgttttaatt ctgaataaaa ccaggagaag ttttgctctt ttctcacttg ctcaaacatc
      301 ttttgaaaaa caacgacctg atccatcagg ataataacct cttgttagtt gtgagactgc
      361 gtagtgtgca cgatcggttt taccacttca atctggtctg tcctttggct gtgatatgta
      421 caaagtgtga aagggggatt acctgaattc tcccggtgag cattttgcaa cggaccagct
      481 ccggtacaaa agctgttgtg ggttcagatt ataagattct gtctaagggg cgggataaag
      541 gtgaaattag ggggcatgaa agatgacttt ataaccttgc tcaccccagt gttgtaaaag
      601 ttcgttttgc cttctcgttg gtgccatgcc tgtccagaca atcaatgttt gcgtcgggaa
      661 cagttccggg cgcggcgatt caatgggatc agcaagaaca gaaatgtgcc atcctgacaa
      721 tgataaacgc catgcttcca gccacaatcg tgctctctct tcaacattcc acgccatcag
      781 aatggcttct ttaccgggct tacggcgcat ttcgaaaagt gaggctgctg cgtattcaat
      841 taatgcgccg tcaaacatac tgctcataat gcgggaggtg ttgtgatcaa gcacgagacg
      901 ctggcgaaca ggaaggtaaa catgattaat caattgatct actgggtact ctcgacccag
      961 tgaaataatt ctcgcgcgta gtttggcagg attagccata cgaagaattg acatcatctc
     1021 ttcttgcagg cgactccagt catcttccgt atcctggctg gtggtttcca gtaatgcttt
     1081 aactttgcct acaggaacgc cattacttat ccaacgcttg atctcttcga tgcgttgtat
     1141 gtcttcttca tcaaagagtc ggtgtccgcc ttcactgcgc tgtggtttta acaaaccgta
     1201 gcggcgttgc caggcccgga gagtgacagg attaatcccg caacgttcag caacatcacc
     1261 aatgctgtaa taagccacaa ttcctccttg cggtcacaaa tctccgtcgc ctgtacacga
     1321 cccaataata ctttgtacaa tatacgct
//
LOCUS       DAZFLD010000489         1327 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.492, whole genome shotgun sequence.
ACCESSION   DAZFLD010000489 DAZFLD010000000
VERSION     DAZFLD010000489.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1327
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.492"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..612
                     /locus_tag="ACHKBL_005107"
     CDS             <1..612
                     /locus_tag="ACHKBL_005107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000174720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO5821816.1"
                     /translation="VTEGRMQPGSETEPGVETQSSVLKMLSSDSELSEKQGQKILSSD
                     SEPSEKSEGYHRVRKPNHYVRNITHSVIKNTYVLPEALTGQIREEEAIMLTSQLQALP
                     EELAQGVLESLSGMLGSHSLQNPVGWLLAVLKRAREGNFYPPKQKQRVVVPASVLAGE
                     RGRATAQSVSSGKRTEPISAERLSGIVAGLRASLRRCSAMRSE"
     gene            724..1203
                     /locus_tag="ACHKBL_005108"
     CDS             724..1203
                     /locus_tag="ACHKBL_005108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5821817.1"
                     /translation="MQKYKQESLSAAMKESGLLIAKICACVIAGVVVIWGLVTGTETL
                     LTALKTMHEQYDNSMCYIVCATGWFFLVLSNWLWVTYSLYKARTGSTVGGGLQFMLLL
                     SLLAECFSFFVIFWSLSHLDITDIQLLVMGITTLMLFLSLNVKVVVIINHVASGNSD"
BASE COUNT          370 a          262 c          391 g          304 t
ORIGIN      
        1 gtgactgagg gcagaatgca gccgggttcg gaaaccgaac cgggggtaga aacacagagt
       61 tcggtattga aaatgctgag ttcggattcc gaactcagtg aaaaacaggg gcaaaaaata
      121 ctgagttcgg attccgaacc atcagaaaaa tcagaaggtt atcacagagt tcggaaaccg
      181 aaccattacg tacgtaatat cacacacagt gtgattaaaa acacgtacgt attgcctgaa
      241 gcgctgacag ggcaaatcag ggaggaggag gccattatgc tgaccagtca gttgcaggcg
      301 ctgccggaag aactggcgca gggggttctg gaaagtctgt cagggatgct gggcagccac
      361 tcgctgcaga atcctgtggg ctggttgctt gcagtgctga agcgagcccg ggagggaaat
      421 ttttatccgc cgaagcagaa acaacgtgtt gttgtgccag cctccgtact ggcaggagaa
      481 agggggcgag ctactgcgca atcagtttca tcggggaaac gtactgaacc gatttcagca
      541 gaacgtctga gtggcattgt ggctggacta cgggcctcgc tgcgcagatg ttctgcgatg
      601 agatctgagt gattgcaata cgggctatgt tcgcgggaac aaaaatcaac cagtgccgcc
      661 agcgactggt taaaaagaaa acagaatcgg gaactggtca ggtgaaacaa ggtggtaaat
      721 agcatgcaga aatacaaaca ggaaagtctg agtgcagcca tgaaagaaag tggcctgttg
      781 atcgctaaaa tttgtgcctg cgtgattgcc ggagtggtgg ttatctgggg gctggtgaca
      841 ggcacagaga ccctgctcac tgcgctgaaa acaatgcacg aacagtacga taacagtatg
      901 tgttatatcg tatgtgccac agggtggttt ttcctggtgt taagtaactg gctgtgggtc
      961 acatacagtc tttataaagc gcgtacaggc agtacagtcg gagggggcct gcaatttatg
     1021 ctcctcctga gtctgctggc agaatgcttt tcattttttg tgattttctg gtcattgtct
     1081 catctggata taaccgacat tcagttgctc gtgatgggga taacgacatt aatgctgttt
     1141 ttgtctctga acgttaaggt agtagtcatc attaaccatg ttgccagtgg caacagtgat
     1201 taaaaaatga gcggtgttgc cagtggcaac agaggttaaa aaatgagcag tgctgccagt
     1261 ggcagcagag gtcaaaaagt gaacagtgct gccagtggca gaagaggtta aaaagtgaac
     1321 agtgctg
//
LOCUS       DAZFLD010000490         1324 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.493, whole genome shotgun sequence.
ACCESSION   DAZFLD010000490 DAZFLD010000000
VERSION     DAZFLD010000490.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1324
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.493"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..362
                     /locus_tag="ACHKBL_005109"
     CDS             <1..362
                     /locus_tag="ACHKBL_005109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001279669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="replication endonuclease"
                     /protein_id="HFO5821818.1"
                     /translation="LTLPEGDLGEWLRSLTPTERKQLTRQLKSPPPDTETVKNGGISA
                     PAGGVRVSVALTAEPHITAQITRELDALGVQLHESAVVSVRHGARVRLADGRTSYWND
                     STRRIDITPAPNTEAPQ"
     gene            359..763
                     /locus_tag="ACHKBL_005110"
     CDS             359..763
                     /locus_tag="ACHKBL_005110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5821819.1"
                     /translation="MTRFYCAMQRQRFDELMRQRTPLLTLNNIATGPQPGDELEITVV
                     SPDRTTTTIITDILNGRPDSTNRSWLVAVRPRRPPGEPEPLTPAGKIMRQYHEEQAHK
                     HNAELTRTLASINRKAAIARYVHSAAGRQSRR"
     gene            812..982
                     /locus_tag="ACHKBL_005111"
     CDS             812..982
                     /locus_tag="ACHKBL_005111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5821820.1"
                     /translation="MNSTRRISDRELTKMIESAQQLLSRQNPVVEETWWENLLAALTE
                     LRERRNNEGAAK"
     gene            979..1305
                     /locus_tag="ACHKBL_005112"
     CDS             979..1305
                     /locus_tag="ACHKBL_005112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000856556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFO5821821.1"
                     /translation="MKTPGMTGKEIILRYLETHARFSAPELVEKLGVKRTVINQAAGK
                     MVREGLLVVDGYTNKITHYRKPTEKERQELAQRAERQQEKSVIEACKRSEIMKRILFI
                     YGAAFR"
BASE COUNT          372 a          392 c          364 g          196 t
ORIGIN      
        1 gacttaccct gccggaaggt gatttgggtg aatggttaag gtcactgacc cccacagagc
       61 gaaaacaact caccaggcag ctgaaaagcc cgccacctga cacggaaacc gtgaaaaacg
      121 gcggcatcag tgcgcccgct ggcggcgttc gcgtgagtgt cgctctgacc gctgagccac
      181 acatcaccgc acaaatcacc cgcgagcttg atgcgctggg cgtgcagctg catgaaagcg
      241 ccgtcgtttc cgtccgtcac ggtgcccgcg tgcgcctggc tgacgggcgc acctcgtact
      301 ggaatgacag cacccgccgc attgatatca cccccgcacc gaatacggag gcgccacaat
      361 gacccgcttt tattgcgcca tgcagcgaca gcgttttgac gaactgatgc gccagcgcac
      421 gcccttactg accctgaaca acattgccac ggggccacaa ccgggcgatg agctggaaat
      481 cacggtcgtc agtcccgatc gcaccaccac gaccatcatc accgacatac tcaacggacg
      541 accggacagc accaaccgca gctggcttgt cgccgttcgt ccgcgccgtc cgcccggaga
      601 gccggagccg ctcacgcctg ccggaaaaat catgcggcag taccacgaag agcaggcaca
      661 caaacacaac gcagaactca ccagaacact ggcatcgatt aaccgcaaag cggcaattgc
      721 ccgctatgtg cattcagccg ctggcagaca atcacgcagg taaacaaaga ccatgaacat
      781 aaccagaaac agccaactaa aggagcgcac catgaacagc accaggagga tcagcgacag
      841 agagcttacg aaaatgattg agtcggcaca gcagctgctt tcacgacaaa acccggtcgt
      901 ggaagaaaca tggtgggaga atttactggc agcactgacc gaactccggg aacgccggaa
      961 caacgaagga gccgccaaat gaagacccca ggaatgaccg gaaaggaaat cattttacgc
     1021 tacctggaaa cgcacgcacg tttcagcgcc ccagaactgg tagaaaaact gggcgtgaaa
     1081 agaaccgtca ttaatcaggc cgccggaaaa atggtacggg aagggttgct ggttgtggac
     1141 gggtacacga acaagataac gcattaccgg aagccaacgg aaaaggaacg acaggaactg
     1201 gcgcaacggg ctgaacggca gcaggaaaaa tcagtcatcg aagcctgcaa gcgcagcgaa
     1261 atcatgaagc gaattctgtt tatctacggc gctgcattcc gttaaaggag ctggtgagtc
     1321 gggg
//
LOCUS       DAZFLD010000491         1318 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.494, whole genome shotgun sequence.
ACCESSION   DAZFLD010000491 DAZFLD010000000
VERSION     DAZFLD010000491.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1318
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.494"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>1318
                     /locus_tag="ACHKBL_005113"
     CDS             <1..>1318
                     /locus_tag="ACHKBL_005113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPD5 domain-containing protein"
                     /protein_id="HFO5821822.1"
                     /translation="SGVYQAAGGKRYDPPQKLYSLRNKDNKGSNLFFSESRDELLAKA
                     KVWFAEQEEKSQAKGDEKTAPSPDDKIRFDVYRNTRSGDIFIAYGKNKMRVRGGFKSA
                     SDARKYIDSHRDELVRHVKEMREISREEQRNATNRDRTGPERRKGNVSPEQFSDAFGF
                     RGVQFGNYVEGPRRQADLNRAYDSLHDLAEVLNVPTKALSLNGRLGLAFGARGKGKAA
                     AHYESGEVAINLTKGNGPGALAHEWFHSLDNYFGRYDVSSDGKITSGGDFMTEAQRVR
                     RIFKDGRYVDAEYPVRQEVYDAFKGVIQAIKNSDMPRRSALLDEVRSKPYWSTDVEMA
                     ARAFERYVQDKARMAGVENDYLVNIRKAPEHNTDNTYAYPTNAELDGGIREAFDHLFR
                     TLKTRETDKGVAFYSRKGVTRTPEGNLISDVNRSAEAKGSPVPQVEA"
BASE COUNT          333 a          289 c          393 g          303 t
ORIGIN      
        1 tcgggtgtgt atcaggcggc aggagggaag cgttacgatc cgccacagaa actttattca
       61 ctgcgcaata aggacaataa ggggagtaac ctctttttct cggaaagcag ggatgaatta
      121 ctggcaaagg cgaaagtctg gtttgcagag caggaggaaa aatcacaggc gaaaggtgat
      181 gaaaaaacag caccgtcacc ggatgacaaa atccgctttg acgtttaccg gaatacccgc
      241 agtggcgata tttttatcgc ttacggtaaa aacaaaatgc gggtgagagg tggctttaag
      301 tcagccagtg atgcgcgtaa gtacattgat tcacatcgtg atgagcttgt tcgtcatgtg
      361 aaggagatgc gggagatttc gcgtgaggag cagcgcaacg ccaccaaccg cgaccgtacc
      421 ggaccagaac gccgcaaggg gaatgtttca ccggagcagt tcagtgatgc gtttggtttc
      481 cgtggtgtgc agtttggtaa ctacgtggaa ggtccgcgtc gtcaggctga tttgaaccgg
      541 gcttatgact cgctgcatga ccttgcggaa gtactgaatg taccgacaaa agcgctttcc
      601 ctgaacggtc gtcttggcct ggcatttggt gcccgtggta agggtaaggc ggcggcacac
      661 tatgagtcag gtgaggtggc aatcaacctg acaaaaggta acggaccggg tgcgctggcg
      721 cacgaatggt tccattctct ggataattat tttggtcgtt atgacgtttc cagtgacggg
      781 aaaattacgt caggtggcga ctttatgacg gaagcacagc gtgtcaggcg catatttaaa
      841 gacggcaggt atgttgatgc tgaatatccg gtacgtcagg aggtttacga cgcttttaaa
      901 ggtgtgattc aggccattaa aaacagtgac atgccgcgtc gttcagcgct tctcgatgag
      961 gtgcgctcaa aaccgtactg gtcaacggat gttgaaatgg cggcacgtgc ctttgagcgt
     1021 tatgttcagg ataaggcgcg tatggctggc gtggagaatg attatctggt caatatccgt
     1081 aaggcacctg agcacaacac agataacacc tacgcttatc cgacgaatgc ggaactggat
     1141 ggcggtattc gtgaggcatt cgatcacctg ttccgcaccc tgaaaacccg tgagacggac
     1201 aagggcgttg cgttttattc ccgtaagggc gttacccgca cacctgaagg taatctcatt
     1261 tcggatgtta accgtagtgc ggaagccaaa ggcagcccgg tcccgcaggt tgaagcgg
//
LOCUS       DAZFLD010000492         1310 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.495, whole genome shotgun sequence.
ACCESSION   DAZFLD010000492 DAZFLD010000000
VERSION     DAZFLD010000492.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1310
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.495"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            92..706
                     /gene="evgA"
                     /locus_tag="ACHKBL_005114"
     CDS             92..706
                     /gene="evgA"
                     /locus_tag="ACHKBL_005114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system DNA-binding response
                     regulator EvgA"
                     /protein_id="HFO5821823.1"
                     /translation="MNAIIIDDHPLAIAAIRNLLIKNDIEILAELTEGGSAVQRVETL
                     KPDIVIIDVDIPGVNGIQVLETLRKRQYSGIIIIVSAKNDHFYGKHCADAGANGFVSK
                     KEGMNNIIAAIEAAKNGYCYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKD
                     NNDIAEKMFISNKTVSTYKSRLMEKLECKSLMDLYTFAQRNKIG"
     gene            711..>1310
                     /locus_tag="ACHKBL_005115"
     CDS             711..>1310
                     /locus_tag="ACHKBL_005115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HFO5821824.1"
                     /translation="MKFLPYIFLLCCGLWSTISFADGDYIEYRGISSNNRVTLDPLRL
                     SNKELRWLASKKNLVIAVHKSQTATLLHTDSQQRIRGINADYLNLLKRALNIKLTLRE
                     YADHQKAMDALEDGEVDIVLSHLVASPPLNDDIAATNPLIITFPALVTTLHDSMRPLT
                     SSKPVNIARVANYPPDEVIHQSFPKATIISFTNLYQALAS"
BASE COUNT          413 a          267 c          256 g          374 t
ORIGIN      
        1 tttcttattt tgattgagaa aatgagatga tgccttatgt ctgtattact acagggagaa
       61 gggaaatgct tcattgcaaa gggaataatc tatgaacgca ataattattg atgaccatcc
      121 tcttgctatc gcagcaattc gtaatttatt gatcaaaaac gatattgaaa tcttagcgga
      181 gttgactgaa gggggaagtg ccgttcagcg ggtggaaaca cttaaacctg atatcgtcat
      241 cattgatgtc gatatccccg gagttaacgg tatccaggtg ttagaaacgc tgaggaagcg
      301 ccaatatagc ggaattatta ttatcgtctc cgctaaaaat gaccattttt acgggaaaca
      361 ttgtgctgat gctggcgcta atggtttcgt gagtaaaaaa gaaggcatga acaatatcat
      421 tgcagctatt gaagctgcaa aaaatggcta ctgctatttc cccttctctc tcaaccggtt
      481 tgttggaagt ttaacgtccg accagcaaaa actcgactcc ttatcgaaac aagaaattag
      541 tgtcatgcgg tatattcttg atggcaagga taataatgac attgctgaaa aaatgttcat
      601 cagcaacaaa actgtcagca cttataaaag tcgcttgatg gaaaaattag aatgcaaatc
      661 actgatggat ctttacacat tcgcacaacg taacaaaatc ggctaaccac atgaagtttt
      721 taccctatat ttttcttctc tgttgtggtc tttggtcgac cataagtttc gcagacggag
      781 attacatcga atatcgtggc atcagtagta acaaccgtgt cacacttgat ccactacgtc
      841 tgagcaacaa ggaattacgt tggctcgcga gcaaaaaaaa tcttgtgatt gcagtacaca
      901 agtcccaaac agctacgttg ttgcataccg attcgcagca acggattcgt ggtattaatg
      961 ctgattattt aaatctttta aaaagagcgt taaatatcaa attaacactc cgggaatacg
     1021 cagatcatca aaaagcaatg gacgcgctgg aagacggtga agttgatata gtgttatcac
     1081 atttagttgc ttcgccgcct cttaatgatg acattgctgc aaccaatcct ctgataatta
     1141 cctttccggc gctggtcact acccttcacg attcaatgcg accgcttacc tcatcaaaac
     1201 cagtaaatat tgctcgagta gcaaattatc ccccagacga ggtaattcat caatcatttc
     1261 caaaagcaac aattatctct tttacaaatt tatatcaggc attagcatcc
//
LOCUS       DAZFLD010000493         1309 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.496, whole genome shotgun sequence.
ACCESSION   DAZFLD010000493 DAZFLD010000000
VERSION     DAZFLD010000493.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1309
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.496"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>1309
                     /locus_tag="ACHKBL_005116"
     CDS             <1..>1309
                     /locus_tag="ACHKBL_005116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HFO5821825.1"
                     /translation="LMKRHLNTCYRLVWNHITGAFVVASELARARGKRGGVAVALSLA
                     AVTSLPVLAADIVVHPGETVNGGTLVNHDNQFVSGTADGVTVSTGLELGPDSDENTGG
                     QWINAGGTGRNTTVTANGRQIVQAGGTASDTVIRDGGGQSLNGLAVNTTLDNRGEQWV
                     HGGGKAAGTIINQDGYQTIKHGGLATGTIVNTGAEGGPESENVSSGQMVGGTAESTTI
                     NKNGRQVIWSSGMARDTLIYAGGDQTVHGEAHNTRLEGGNQYVHNGGTATETLINRDG
                     WQVIKEGGTAAHTTINQKGKLQVNAGGKASDVTQNTGGALVTSTAATVTGTNRLGAFS
                     VVAGKADNVVLENGGRLDVLSGHTATNTRVDDGGTLDVRNGGAATTVSMGNGGVLLAD
                     SGAAVSGTRSDGTAFRIGGGQADALMLEKGSSFTLNAGDTATDT"
BASE COUNT          319 a          328 c          428 g          234 t
ORIGIN      
        1 ctgatgaaac gacatctgaa tacctgctac aggctggtat ggaatcacat tacgggcgct
       61 ttcgtggttg cctccgaact ggcccgcgca cggggtaaac gtggcggtgt ggcggttgca
      121 ctgtctcttg ccgcggtcac gtcactcccg gtgctggctg ctgacatcgt tgtgcacccg
      181 ggtgaaacag tgaatggcgg aacactggta aaccatgaca accagtttgt atccggaaca
      241 gctgatggcg tgactgtcag taccgggctt gagctggggc cggacagtga cgaaaacacc
      301 ggcgggcaat ggataaacgc gggtggcaca ggcagaaaca ccactgtcac cgcaaatggt
      361 cgtcagattg tgcaggcagg aggaactgcc agtgatacgg ttattcgtga tggcggaggg
      421 cagagcctta acggactggc ggtgaacacc acgctggata acagaggtga gcagtgggta
      481 cacgggggag ggaaagctgc cggtacaatt attaaccagg atggttacca gaccataaaa
      541 catggcggac tggcaaccgg aaccatcgtc aacaccggtg cagaaggtgg tccggagtct
      601 gaaaatgtgt ccagcggtca gatggtcgga gggacggctg aatccaccac catcaacaaa
      661 aatggccggc aggttatctg gtcttcgggg atggcacggg acaccctcat ttacgccggt
      721 ggtgaccaga cggtacacgg agaggcacat aacacccggc tggagggggg taaccagtat
      781 gtacacaacg gtggcacggc aacagagacg ctgataaacc gtgatggctg gcaggtgatt
      841 aaggaaggag gaactgccgc gcataccacc atcaaccaga aaggaaagct gcaggtgaat
      901 gccggcggta aagcgtctga tgtcacccag aacacgggcg gtgcactggt taccagtaca
      961 gctgcaaccg tcaccggtac aaaccgcctg ggagcattct ccgttgtggc gggtaaggct
     1021 gataatgtcg tactggaaaa tggcggacgt ctggatgtgc tgagcggaca cacagccact
     1081 aatacccgtg tggatgatgg cggaacgctg gatgtccgca acggtggtgc cgccaccacc
     1141 gtatccatgg ggaatggcgg tgtactgctg gccgattccg gtgccgctgt cagtggtacc
     1201 cggagcgacg gaacggcatt ccgtatcgga ggcggtcagg cggatgccct gatgctggaa
     1261 aaaggcagtt cattcacgct gaacgccggt gatacggcca cggatacca
//
LOCUS       DAZFLD010000494         1307 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.497, whole genome shotgun sequence.
ACCESSION   DAZFLD010000494 DAZFLD010000000
VERSION     DAZFLD010000494.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1307
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.497"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..396)
                     /locus_tag="ACHKBL_005117"
     CDS             complement(<1..396)
                     /locus_tag="ACHKBL_005117"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5821826.1"
                     /translation="MTTCSELKERARFDGMHDASLEVAWQLLMTKSVSTITTEIISGA
                     DMSNFSVIIRERYKHAIAKSLLNHLSPDVVSDIIGLSHDEFNQQYKGNLTRQQHLRDI
                     AVSLTDKGFTESSACNIVNLDLMLLPEENW"
     gene            complement(480..>1307)
                     /locus_tag="ACHKBL_005118"
     CDS             complement(480..>1307)
                     /locus_tag="ACHKBL_005118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000033404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III restriction-modification system
                     endonuclease"
                     /protein_id="HFO5821827.1"
                     /translation="KSFMIEETDRFTKSGVHTRIDRIYIHNDTAMSKSIVSDDDNFAK
                     LNTMSYREFLDNLSQTIFAKHDTLHKVFCDIKDTINITDYLNIQTIRKIKSGFSKYLL
                     NNSFNKFSLGYNVISGSIHPTKFTNADGNHLHEVLSGDLGVLQDNTKAPLDSYLFEEV
                     FYDSELERLNITDGEIHSVAVFTKIPKNSIKIPVAGGYTYSPDFAYVVKTTKGDYLNF
                     IIETKNVEGKESLRKEELDKIKHAQKLFEQVSKSVKVTFITQFSGDRIHDLIKNVIQ"
BASE COUNT          387 a          224 c          230 g          466 t
ORIGIN      
        1 ccagttttct tcaggaagta acattaggtc caggttgaca atattacatg cagatgattc
       61 tgtgaatcct ttatctgtta aacttacagc aatatctctc aggtgttgtt gtctggttaa
      121 attaccttta tattgttgat tgaactcatc atgagataaa ccgatgatgt cagaaacaac
      181 atcgggagat aaatgattga gaagagattt tgctattgca tgtttatatc gttccctgat
      241 aatcactgaa aaattggaca tgtctgcacc ggatattatc tcagtagtga tagtggatac
      301 acttttcgtc ataagtaatt gccatgccac ttcaagagaa gcatcatgca taccatcaaa
      361 acgggctctt tccttcagtt cagaacatgt tgtcattgaa ctcacctgcg aattttgtga
      421 tgtgtgtata attataaagt gggggtgaaa ccacctccac tttgttatca tgcatgtgtt
      481 tactgaatca cattttttat cagatcatgg attctgtcac ctgagaattg agttatgaaa
      541 gttactttta cagatttact tacctgttcg aaaagttttt gtgcatgctt tattttatcc
      601 agttcttctt tacgtaaact ttctttacct tcgacatttt ttgtttcaat aataaagtta
      661 aggtagtcac cctttgttgt ttttacaaca taagcaaagt caggagaata tgtgtatcca
      721 cctgccaccg gaattttaat tgagtttttc ggtatctttg taaaaacagc aacagagtgg
      781 atctctccgt ctgttatatt gagtctttca agctctgaat cataaaaaac ttcttcaaaa
      841 agataagagt ccaggggggc ttttgtatta tcctgtaata ctccaagatc acctgataat
      901 acctcatgca ggtgattacc gtctgcatta gtgaattttg taggatgaat tgagcctgaa
      961 atcacattat atccaaggct gaatttgtta aatgaattgt tcaacaaata cttgctaaac
     1021 ccagatttaa tctttctgat tgtctgaatg ttcaggtagt cggtgatatt gatagtgtct
     1081 tttatatcac aaaaaacctt atgtaaggta tcgtgtttag caaaaatagt ttgtgataag
     1141 ttatcaagaa attcccgata gctcattgtg tttagtttag caaagttatc atcgtcactg
     1201 acgatacttt tcgacatcgc cgtatcatta tgaatataaa ttctatcaat gcgagtatga
     1261 acacctgatt tagtaaatct atctgtttct tcaatcatga atgattt
//
LOCUS       DAZFLD010000495         1306 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.498, whole genome shotgun sequence.
ACCESSION   DAZFLD010000495 DAZFLD010000000
VERSION     DAZFLD010000495.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1306
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.498"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>1306)
                     /locus_tag="ACHKBL_005119"
     CDS             complement(<1..>1306)
                     /locus_tag="ACHKBL_005119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="S-type pyocin domain-containing protein"
                     /protein_id="HFO5821828.1"
                     /translation="DWLAFPAGYNSLFIPPKSPPASNLKPEPLPVVNLPLSSPAVNPV
                     YAKSCLKEKGCTDAGTAEEPAENFGQVAIFAQPVVDDCCGYHHPEANDVGQPAEAQTM
                     LLFPGSVLAAQIWGKWSLSGILSATRGSYIGALASALYIPSAGEGSARVPGRDEFWYE
                     EELRQKALAGSTATTRVRFFWGTDIHGKPQVYGVHTGEGTPYENVRVANMQWNEQTQR
                     YEFTPAHDVDGPLITWTPENPEHGNVPGHTGNDRPPLDQPTILVTPIPDGTDTYTTPP
                     FPVPDPKEFNDYILVFPAGSGIKPIYVYLKEDLRKLPGVVTGRGVPLSLGTRWLDMSV
                     SNNGNGAPIPAHIADKLRGREFKTFDEFREALWLEVSKDSVLLAQFIKSNQNNVSQGY
                     SPYVPEEGYYYGPNEIVKKFQIHHVVAIEHGGGVYDIDNFRIV"
BASE COUNT          321 a          354 c          317 g          314 t
ORIGIN      
        1 acaatcctaa aattatcaat atcatacact ccaccaccat gttctatagc aactacatga
       61 tgtatctgga atttcttaac tatctcattt ggtccgtaat aatacccctc ctcagggaca
      121 tagggggaat atccttgact aacgttattc tgattacttt ttataaactg cgccaataac
      181 actgagtctt tactcacctc cagccacagc gcctcgcgaa actcatcaaa ggttttaaac
      241 tcccgtccgc gcaatttatc agcaatatgc gccgggattg gtgcgccgtt gccgttattg
      301 gataccgaca tatccagcca gcgagttcct agtgaaagcg ggacgccgcg ccctgttaca
      361 acaccaggca gctttcgcag atcctccttc aggtaaacat agatgggctt aataccggat
      421 cccgccggaa aaaccagaat ataatcgttg aattctttcg gatcaggaac cgggaatggc
      481 ggcgtggtat aggtatcggt gccgtccgga atcggcgtca ccagaatggt gggctgatcc
      541 agcggcggcc tgtcgttacc ggtatggccc ggaacattcc catgttccgg attttccggc
      601 gtccaggtaa tcagggggcc atcgacatcg tgagcggggg taaattcata acgctgcgtc
      661 tgctcgttcc actgcatgtt cgccacgcgg acgttttcat acggcgtacc ttcacccgta
      721 tgaacaccat acacctgagg cttgccgtga atgtcagttc cccagaaaaa acgtacccgg
      781 gtggtggcgg tactgcctgc tagtgctttc tgccgcagtt cttcctcata ccagaactca
      841 tcacgtccgg gcacgcgagc actgccctcg cccgcagagg gaatatacaa agcagatgcc
      901 aacgcaccga tgtaagagcc gcgggttgca ctgagtatgc cactgagcga ccattttccc
      961 catatttgag ccgccaacac actacccgga aacagtagca tggtttgagc ttctgcgggt
     1021 tgcccgacat cgttagcctc gggatggtgg tatccacagc agtcatcaac cactggctga
     1081 gcaaaaatcg ctacttgacc gaagttttca gcgggttctt ctgccgttcc agcatctgta
     1141 catcccttct ccttcagaca ggattttgca tagaccgggt taaccgctgg tgaagagagt
     1201 ggaaggttaa caactggtag aggttcaggc ttaagattac tggcaggtgg gcttttaggc
     1261 ggaataaata agctattata gccagcagga aaggccaacc agtcaa
//
LOCUS       DAZFLD010000496         1284 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.499, whole genome shotgun sequence.
ACCESSION   DAZFLD010000496 DAZFLD010000000
VERSION     DAZFLD010000496.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1284
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.499"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            280..543
                     /gene="ykgM"
                     /locus_tag="ACHKBL_005120"
     CDS             280..543
                     /gene="ykgM"
                     /locus_tag="ACHKBL_005120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HFO5821829.1"
                     /translation="MKPNIHPEYRTVVFHDTSVDEYFKIGSTIKTDREIELDGVTYPY
                     VTIDVSSKSHPFYTGKLRTVASEGNVARFTQRFGRFVSTKKGA"
     gene            543..683
                     /gene="ykgO"
                     /locus_tag="ACHKBL_005121"
     CDS             543..683
                     /gene="ykgO"
                     /locus_tag="ACHKBL_005121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HFO5821830.1"
                     /translation="MKVLNSLRTAKERHPDCQIVKRKGRLYVICKSNPRFKAVQGRKK
                     KR"
     gene            complement(718..943)
                     /gene="ykgL"
                     /locus_tag="ACHKBL_005122"
                     /pseudo
     CDS             complement(718..943)
                     /gene="ykgL"
                     /locus_tag="ACHKBL_005122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414829.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YkgL"
     gene            1090..>1284
                     /locus_tag="ACHKBL_005123"
     CDS             1090..>1284
                     /locus_tag="ACHKBL_005123"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5821831.1"
                     /translation="MQCPGKEAIVPVTTGSLRLTSLNLALGGLTGQMTYLHLFNFSSL
                     IGMNTHNHFVRHVNPLKHLTD"
BASE COUNT          367 a          288 c          270 g          359 t
ORIGIN      
        1 aggaatacac attgtcaggt aatcaatcat gctgcaataa atcatcggcc agtaaagtgg
       61 agatagcctc cattctcgaa aaatccatac tctcagcgaa accatcatca atcactcatc
      121 caggcgttta tgggagcgtc gccaatggct gctaacaatg ccagacttcc ccgttgcgga
      181 aattccacat cccacaaata gtcacagtga ttgggtgttg aaatgatccg gatgagcatg
      241 tatctttacg gttatgttat aacataacag gtaaaaatga tgaaacccaa tatccatcct
      301 gagtatcgta ctgtggtgtt ccacgacacc agcgttgatg agtactttaa aatcggctcg
      361 actatcaaaa cagaccgtga gattgagctg gatggcgtaa cgtatccgta cgtaacaatt
      421 gatgtctctt cgaaatcgca cccgttctat acagggaagc tgagaacagt ggcatcagaa
      481 ggaaatgttg cacggttcac ccaacgtttt ggtcgttttg ttagcacgaa aaagggggca
      541 tgatgaaagt ccttaactct ctgcgtaccg caaaagaacg ccatccagac tgtcagattg
      601 tgaagcgaaa aggacggctg tatgtgattt gtaaatctaa tccacgtttt aaggccgttc
      661 agggtcgtaa gaaaaaacgt tgattcaaaa ttcgacgaat taacgatatt tgtctgatta
      721 ataatcatac cggattaatg ttggtgtatt tattacacca gcattaattt tcctggggat
      781 gtatcttcct gttaatttaa ggtcaactgc ctgacgtttc tctccgaata ttccattata
      841 ttaatgttga cttgttgacc agctgcgccc tgtatgctgg catcaactct attttagact
      901 gaacacgcca ctcagtttcc tccctttgcg acgcagcctg catttacact caaactgtta
      961 agatgataaa tgtggtaaat ctgttggtac tgacataaaa acgtttacgc cacaggaacg
     1021 gccagatcca tcggtaaccc catcgccgac gttcgagtgc cagttagagt aacgcgtaca
     1081 gatgactgaa tgcagtgccc tggcaaagag gccatcgttc ctgtgacaac tggcagtctt
     1141 cgtttaactt cacttaattt ggctcttggg gggcttaccg gacagatgac gtacttacac
     1201 ctgtttaatt tttcatcact tattgggatg aacacccaca accattttgt gcggcatgtt
     1261 aatccattaa aacaccttac tgat
//
LOCUS       DAZFLD010000497         1265 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.500, whole genome shotgun sequence.
ACCESSION   DAZFLD010000497 DAZFLD010000000
VERSION     DAZFLD010000497.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1265
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.500"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1243
                     /locus_tag="ACHKBL_005124"
     CDS             <1..1243
                     /locus_tag="ACHKBL_005124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO5821832.1"
                     /translation="ALVCFITVINYLDRTALGIAAPTIMETTGITKEQYSWIVSAFQL
                     AYTLGQPIMGFFIDTVGLKLSFAICAAIWGLATMGHALTGTWSGLAFMRALMGFSEAS
                     AIPAGVKTASTWFPAKERGVATGVFNMGTSLGAMLAPPLIAWCIMFHSWQFAFIVSGS
                     LALLAALFWFFCYKDPKDAKRLSDEERHYIESGQEQHLKTDKKEKTSIKHILSQRNFW
                     GIGIARFLADPAWGTINFWVPIFFVETLHFSLKEIAMFVWLPFLLGDLGCLASGFVAK
                     FFHDRGVSLINSRRITFTIAAVIMMTIGLVSIVENPYIAVLLISIGAFSHQCLSTVAA
                     TLGGDLFKKDEVATAVGMAGACAWSGQLIFNLFIGAFVHIIGFAPFFIALAFFDIIGA
                     IALWMLIKVKDEEPQVQLATS"
BASE COUNT          302 a          281 c          310 g          372 t
ORIGIN      
        1 tgccctcgta tgctttatca ctgtaatcaa ttatctggac agaacggcgt taggtattgc
       61 ggctccaacg attatggaga caaccggaat aaccaaagaa caatattcat ggattgtcag
      121 tgcattccag ttggcctata cattggggca accgataatg ggcttcttta ttgataccgt
      181 gggtctgaag ttaagttttg cgatatgtgc cgcaatctgg ggcctggcga caatggggca
      241 tgccttgaca ggaacatggt caggtctggc atttatgcgc gccctgatgg gtttcagcga
      301 agcatcggca attcctgcgg gtgtaaaaac cgcatcaaca tggttcccgg caaaagagcg
      361 tggcgtggcg acaggtgttt tcaatatggg cacctcactc ggcgcgatgc ttgctccacc
      421 gttgattgcc tggtgcatta tgtttcatag ctggcaattt gcgtttattg tctcaggtag
      481 ccttgcttta ctcgcggctt tattttggtt cttttgttat aaagatccaa aagatgctaa
      541 acgcctttct gatgaagagc gccactatat tgagtctggg caagaacagc atcttaaaac
      601 agataagaaa gaaaaaacgt caatcaagca tatcctcagc caacgtaatt tctgggggat
      661 tggcatcgcg cgttttctcg ctgacccggc atggggaacc attaacttct gggtaccgat
      721 tttcttcgtc gaaacgctgc attttagcct gaaagaaatt gccatgttcg tctggctacc
      781 tttcctgctg ggcgatctcg gctgtttagc cagtggtttt gtcgcgaagt tcttccacga
      841 tcgcggcgtg agtttaatta actcacgaag aattaccttc actattgcag ccgtcattat
      901 gatgacgatt ggtctggtga gtattgtcga aaatccctac atcgccgtat tactgattag
      961 cattggcgca ttctcgcatc aatgtctttc tactgttgca gcaactctgg gcggtgacct
     1021 attcaaaaaa gacgaagtag ctaccgctgt gggtatggca ggagcctgtg catggagcgg
     1081 tcagttgatt ttcaacctgt ttatcggggc attcgttcac attattggct tcgcgccgtt
     1141 ctttattgcc ctggctttct ttgacattat tggcgccatt gcgctgtgga tgcttatcaa
     1201 agttaaagac gaagaaccac aagtacagtt agcgacaagc taattatttt acatagaaac
     1261 gctat
//
LOCUS       DAZFLD010000498         1262 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.501, whole genome shotgun sequence.
ACCESSION   DAZFLD010000498 DAZFLD010000000
VERSION     DAZFLD010000498.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1262
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.501"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            94..1176
                     /locus_tag="ACHKBL_005125"
     CDS             94..1176
                     /locus_tag="ACHKBL_005125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpD"
                     /protein_id="HFO5821833.1"
                     /translation="MKKLTVAISAVAASVLMAMSAQAAEIYNKDSNKLDLYGKVNAKH
                     YFSSNDADDGDTTYARLGFKGETQINDQLTGFGQWEYEFKGNRAESQGSSKDKTRLAF
                     AGLKFGDYGSIDYGRNYGVAYDIGAWTDVLPEFGGDTWTQTDVFMTGRTTGVATYRNN
                     DFFGLVDGLNFAAQYQGKNDRTDVTEANGDGFGFSTTYEYEGFGVGATYAKSDRTNNQ
                     VIYGNNSLNASGQNAEVWAAGLKYDANNIYLATTYSETQNMTVFGNNHIANKAQNFEV
                     VAQYQFDFGLRPSVAYLQSKGKDLGAWGDQDLVEYIDVGATYYFNKNMSTFVDYKINL
                     IDKSDFTKASGVATDDIVAVGMVYQF"
BASE COUNT          368 a          264 c          278 g          352 t
ORIGIN      
        1 aacttacatc ttgaaataat cacattgatt agatgaatat ttatcgcgca gtgacatcat
       61 tttttaataa tagttcaaaa aaaagggctc acgatgaaaa aattaacagt ggcaatttct
      121 gctgtagctg catcagtact gatggcgatg tctgctcagg cagctgaaat ttataataaa
      181 gacagtaaca agctggatct atatgggaaa gttaatgcca agcactactt ctcctctaat
      241 gatgcagatg atggtgatac tacttatgcc cgtcttggct tcaaaggtga aacccaaatc
      301 aacgatcaac tgactggttt cggtcagtgg gaatatgaat tcaaaggcaa ccgcgctgaa
      361 tctcaaggct cctccaaaga caaaacccgt cttgcatttg caggcctgaa attcggggac
      421 tacggctcaa tcgattacgg ccgtaactac ggtgtagcat atgacatcgg tgcgtggact
      481 gacgttctgc cagaattcgg tggcgatacc tggacccaaa cagatgtgtt catgactggt
      541 cgcaccactg gtgttgcaac ttatcgtaac aacgacttct ttggtctggt tgatggtctg
      601 aactttgctg ctcagtatca gggtaaaaat gaccgcactg acgtaactga agctaatggt
      661 gatggtttcg gtttctccac tacttatgag tatgaaggat tcggtgtagg tgcaacctat
      721 gctaaatctg accgcactaa taatcaggtt atctacggta acaacagcct gaatgcatct
      781 ggtcaaaatg ctgaagtatg ggcagctggt ctgaaatatg atgcgaacaa catctatctg
      841 gctaccacct attctgaaac ccagaacatg actgtttttg gtaataacca tattgccaac
      901 aaagcacaaa acttcgaagt agttgcacaa tatcagttcg acttcggtct gcgtccgtcc
      961 gttgcttacc tgcaatctaa aggaaaagac ttgggtgcgt ggggtgatca ggacctggtt
     1021 gaatatattg atgtaggtgc aacctattac ttcaacaaaa atatgtccac ttttgttgat
     1081 tacaaaatca acctgattga taagagcgat ttcacgaaag catctggcgt tgctaccgat
     1141 gatatcgttg ctgtaggtat ggtttaccag ttctaatttg attactaaaa gatatgttgc
     1201 gggaggcttt gcctccccaa catataagtg gctccctcaa gccacttcct ttaggagcac
     1261 aa
//
LOCUS       DAZFLD010000499         1260 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.502, whole genome shotgun sequence.
ACCESSION   DAZFLD010000499 DAZFLD010000000
VERSION     DAZFLD010000499.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1260
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.502"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            247..798
                     /gene="ybcL"
                     /locus_tag="ACHKBL_005126"
     CDS             247..798
                     /gene="ybcL"
                     /locus_tag="ACHKBL_005126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Raf kinase inhibitor-like protein YbcL"
                     /protein_id="HFO5821834.1"
                     /translation="MKTLIVSTVLAFITFSAQAAAFQVTSNEIKTGEQLTTSHVFSGF
                     GCEGGNTSPSLTWSGVPEGTKSFAVTVYDPDAPTGSGWWHWTVVNIPATVTYLPVDAG
                     RRDGTKLPTGAVQGRNDFGYAGFGGACPPKGDKPHHYQFKVWALKTEKIPVDSNSSGA
                     LVGYMLNANKIATAEITPVYEIK"
     gene            808..>1260
                     /locus_tag="ACHKBL_005127"
     CDS             808..>1260
                     /locus_tag="ACHKBL_005127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFO5821835.1"
                     /translation="MLARGKTNLKIEEIRMHKHHEIHRVKPLMPALCRIRQGKKVINW
                     ETHTLTVDNNQIILFPCGYEFYIENYPEAGLYLAEMLYLPIDLIESFQKLYTVTDQIR
                     NKTSFFLPQNPELIYCWEQLKTSVSRGFSTKIQEHLAMGVLLSLGVNHV"
BASE COUNT          394 a          205 c          273 g          388 t
ORIGIN      
        1 atgagcaaaa tggtgtatgt cacgtatttt gaatggtagg ttaaaaaata acaccgactt
       61 tcgtaggtat tactaataat aaagcagagt ttttagatag tatcaatgtg ctttgtgtat
      121 attgtggcaa ataattgggt tgggggtaca attgtgattg cttttgcatg aacattgcgc
      181 ctttatgcat aatgagataa aggaatatca aataaaataa cgataggtca taacaaagag
      241 gtttttatga aaacacttat cgtttcaact gtattggcat tcataacatt ttctgcgcag
      301 gctgcagcat ttcaggtcac tagtaatgaa ataaaaacag gagagcaact tacaacgtct
      361 catgtctttt ctggatttgg gtgtgaaggt ggtaatacat cgccctcatt aacctggtct
      421 ggtgttcctg aaggtaccaa aagctttgcc gtaactgtat atgatccaga tgcacctaca
      481 ggcagtggtt ggtggcattg gactgttgtt aatattccag caacagtaac atatttgccc
      541 gttgatgcag ggagacgtga tggaacaaaa ctgccgactg gtgctgttca aggccgaaat
      601 gattttggct atgctgggtt tggtggcgca tgtcctccta aaggagataa accacatcat
      661 taccagttta aagtatgggc tctaaaaact gaaaagattc ctgtagattc taactccagc
      721 ggagcgttag ttggttatat gcttaatgct aataaaatcg caaccgctga gataacacca
      781 gtttatgaga taaagtaggg tgagagtatg ctggcaagag gtaagactaa cttaaagatc
      841 gaagaaatac ggatgcataa acatcatgag attcataggg ttaagcctct tatgccagct
      901 ttgtgtcgta tccgtcaggg aaagaaagtt atcaattggg agacgcatac tttaactgtt
      961 gataataatc aaataatatt atttccttgt ggttatgaat tttatattga gaattatcct
     1021 gaagcagggc tttatcttgc agaaatgctt tacttaccca ttgatttaat tgagagtttc
     1081 caaaaacttt atacggtaac tgatcaaata cgtaacaaaa caagtttctt tttacctcag
     1141 aatcctgagt taatatattg ttgggagcaa ctaaaaacat ctgtttcccg aggcttctca
     1201 actaaaattc aggagcactt agcaatgggc gttctacttt cgttaggagt gaatcatgtt
//
LOCUS       DAZFLD010000500         1252 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.503, whole genome shotgun sequence.
ACCESSION   DAZFLD010000500 DAZFLD010000000
VERSION     DAZFLD010000500.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1252
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.503"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..249
                     /locus_tag="ACHKBL_005128"
     CDS             <1..249
                     /locus_tag="ACHKBL_005128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5821836.1"
                     /translation="PPDGAEGLKQDIGDLIRCAFRENARFNVKKVWPYSRFSFSTLGK
                     EIHQTFPVVASVQFSLEDIISDLAVPRLESLTVEVVIE"
     gene            242..790
                     /locus_tag="ACHKBL_005129"
     CDS             242..790
                     /locus_tag="ACHKBL_005129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO5821837.1"
                     /translation="MNDFLQRLRSLKLPSWMDRGEPDKLLRAARAFWSRVWEWITWPV
                     RQFDPMTCAEPVLDLLAWERDITRFKGEPTEIYRRRISYAFINAQEAGEVAGFIAIFE
                     RLGIGYVELLERQPGVDWDVILVRVTDSQIADNSDLLLEIIRKYGRTCRRYRFEVETL
                     TPFIIHYGAYEGEYICWTARAE"
     gene            813..>1252
                     /locus_tag="ACHKBL_005130"
     CDS             813..>1252
                     /locus_tag="ACHKBL_005130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO5821838.1"
                     /translation="MAKSVITSAFIRLKAQQAAEGTPVVLDEFIFANVPDLNPETPVS
                     PDETLPPEDQIVHRQAVGKTGVVNENAVVYSVTLGAELGDFEFNWVGLVSKATSTLAM
                     IVHAPTQKKIRTHNGNEGNVLVRSMLMEYSGAKEATGITTPAQT"
BASE COUNT          280 a          341 c          367 g          264 t
ORIGIN      
        1 ccaccggacg gcgcggaagg gctgaaacag gatatcggcg acctgatacg ctgcgctttc
       61 cgggagaacg cccgctttaa tgtgaaaaaa gtctggccct attcgcgctt ttcgttttcc
      121 acgctgggca aggaaattca tcagaccttc ccggtggtgg cttcggttca gttttcactg
      181 gaagacatca tcagcgatct ggccgtgcca cggctcgaat ccctgacagt ggaggtggtc
      241 attgaatgac tttttgcagc ggcttcgctc gctgaaactg ccctcatgga tggacagggg
      301 agaacccgac aaactgctgc gggccgcccg ggcgttctgg tcccgcgtat gggaatggat
      361 cacctggccc gtgcgccagt ttgaccccat gacatgtgca gagcctgtac ttgatttgct
      421 ggcatgggag cgggacatca cccgctttaa aggggaaccc acggagatct accgccgccg
      481 catcagttac gccttcatca atgcacagga ggctggcgag gtggcgggct ttatcgccat
      541 ttttgaacgg ctgggcatcg gctacgtgga gttactcgag cgccagcccg gcgtggactg
      601 ggatgtgatc ctggtgcggg tgacagacag ccagatagcc gacaacagcg atcttcttct
      661 ggagatcatc cgcaaatatg gcaggacatg ccgccgttac cgttttgaag tggaaaccct
      721 cacccccttc atcatccatt atggcgcata cgaaggggag tatatctgct ggacggccag
      781 ggcagagtga atgactgaag gagtatgact ttatggcgaa atctgtaatt accagcgcct
      841 ttatccggct caaggcacag caggcggcgg aaggcacccc cgttgtgctg gatgagttta
      901 tttttgcaaa cgtcccggac ctcaacccgg aaacgccggt atcccccgac gaaaccctgc
      961 cgcctgaaga tcagattgtt caccgccagg ccgttggtaa aaccggcgtg gtgaatgaga
     1021 atgccgtggt ttattcggtc accctggggg cagagctggg tgattttgaa tttaactggg
     1081 tgggactggt cagcaaagca accagcacgc tggccatgat tgttcacgcc cccacacaga
     1141 aaaaaatcag aacccacaac ggtaacgaag gtaatgtgct ggtccgttcc atgctcatgg
     1201 agtacagcgg tgcgaaggaa gccacaggta tcaccacccc ggcgcagacc tg
//
LOCUS       DAZFLD010000501         1247 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.504, whole genome shotgun sequence.
ACCESSION   DAZFLD010000501 DAZFLD010000000
VERSION     DAZFLD010000501.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1247
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.504"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(77..902)
                     /locus_tag="ACHKBL_005131"
                     /pseudo
     CDS             complement(77..902)
                     /locus_tag="ACHKBL_005131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(1053..>1247)
                     /locus_tag="ACHKBL_005132"
     CDS             complement(1053..>1247)
                     /locus_tag="ACHKBL_005132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000899430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5821839.1"
                     /translation="LLSEKRAESVRGWFIARGFRAERIHATGRGYDAPVVFCPDVEGE
                     ALKECLRPNRRVEITGQSVR"
BASE COUNT          284 a          336 c          291 g          336 t
ORIGIN      
        1 cggcaggaaa agtcatctgg tgtttcgccg gagtgagggg tacaggcaat agcggacata
       61 accatttgtt ctttttctaa gcagccatct gataatattt tatcctgaag gctgtcgggg
      121 agatattccc cagacgagag tgacgacgct gacgattgta gaaaatctca atgtattccc
      181 gtattactga gatggcctca tcccggcttt taaaacgata gtggctcaga ctttcatttt
      241 tcagcgttcc ccagaagctt tccatcggag cattgtcgta acagttaact ttacgcgaca
      301 ttgatatttt cagaccaaac tgctcctgta tggccctgta atcgtatgcg cagtactgtg
      361 aaccacgatc ggagtggtgg attagcccgg taggtgagcg ctggttcctg agcgccataa
      421 acagggcttt acctgtcagc tcttttgtca tgcgctctcc catggcgtag ccgacaattt
      481 cgcacgtata aacatctttg acgccagcga tatacagcca tccttccggg gggcaacata
      541 cgttatatct gcaaccaaga cctgattagg tgctgtaggc gtaaagttct gattcaggag
      601 attcggcgca accggcagat tgtgaccaga gttcgtgttc gccctgaact tgcgtttcag
      661 cttacagtgc agcctcagct ccttacgaag acgtgccagt ctgtcacgac cgacgataat
      721 tccgttatct gccagctccg tctggagcag ctaggttcca tatgtttcgc gtgtgcggat
      781 atgtgccacc ttaatctcca gttttagccg ctcatcactt tgttttctgt ctgagggtca
      841 tgctgtactc agtcgtaata accgatcctg tatacaccaa gtacccgaca catcgcttca
      901 atacataatc gcatctgaat cgttcaccga ttcgcggcat ttttcaaggt agttgtcgcc
      961 attgttcgac taactgatcg acattacagc ctagagccta gctgggggct gtgcacaaaa
     1021 aagccccgga cattccgggg ctacactgca tattaacgaa cgctctgtcc ggttatttca
     1081 accctgcggt tggggcgcag acattccttc agtgcttcgc cctccacatc cgggcagaac
     1141 accactggtg catcatatcc acggcccgtt gcatgtatcc tttcagccct gaatccccgg
     1201 gcaataaacc accccctgac tgattccgcc cgtttctctg acaggag
//
LOCUS       DAZFLD010000502         1242 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.505, whole genome shotgun sequence.
ACCESSION   DAZFLD010000502 DAZFLD010000000
VERSION     DAZFLD010000502.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1242
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.505"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..915
                     /locus_tag="ACHKBL_005133"
     CDS             <1..915
                     /locus_tag="ACHKBL_005133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFO5821840.1"
                     /translation="GTVQRDNSAELLLDYIRRALDQGHSDYPMMSGCTRFMLSPDTRV
                     VAVDLNNVAGDKTPAGRLRTGIMYLLAGQIAGGDFVLPQYQEEIRKNLDPRYHEVIFR
                     RIEQLDQEVKTKVYDELHNAKGINFIWEALDTQELEQRKFGIRTVLSTQLLQDYPPAV
                     LKSANTLWLLRYRPDEIPFLRDNFGVPEVTLRRFLKMPEGAAPDGSGVPVLAVFRVKN
                     GTLARILKFTLGPLELWALNSSPKDSALRRALTQELGSLRARQILAENFPRGSATSLI
                     EHRARTHDSENVIHDLAAELIRKQGYHL"
     gene            915..>1242
                     /locus_tag="ACHKBL_005134"
     CDS             915..>1242
                     /locus_tag="ACHKBL_005134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraV"
                     /protein_id="HFO5821841.1"
                     /translation="MYSIHITPRCRRPARGQVFLKTLRVDGDSFSFTLPSACFTLRPS
                     PNPRVIFASDRRADGEMSGVRLALPGETGTLITTATWQVNVPERPPFDIQHVIHWLLP
                     SPLTALM"
BASE COUNT          268 a          381 c          338 g          255 t
ORIGIN      
        1 ggcacagttc agcgcgataa ctcagcagaa ttgttactgg actacatccg ccgtgccctc
       61 gatcagggac acagtgacta cccgatgatg tccggctgta cccggtttat gctcagcccc
      121 gacacccgcg ttgtggccgt tgatctgaac aatgtggccg gtgacaagac acctgccggg
      181 cgtctgcgca ctggcatcat gtacctgctg gcaggacaaa tcgccggcgg ggattttgtc
      241 ctgccgcagt atcaggagga aatacggaaa aatctcgacc cacgctacca tgaggtgatt
      301 ttccgccgca ttgaacagct tgatcaggag gtcaagacca aagtctatga cgaactgcac
      361 aatgcaaaag ggatcaattt catctgggaa gccctggaca cccaggagct ggagcagcgt
      421 aagttcggga tacgcacagt gctgagcacc cagctattgc aggactatcc accggcagtt
      481 ctcaaatccg ccaacaccct ctggttgctg cgttaccgcc ctgacgaaat tccgtttctg
      541 cgggataatt ttggtgtccc tgaagtcacc ctgcggcgct tcctgaaaat gccggaaggg
      601 gctgcaccgg atggcagcgg tgtcccggta ctggctgtgt tccgtgtaaa aaacggtacg
      661 ctggcccgga tcctgaaatt cactctcggt ccactggagc tgtgggcact gaattcctca
      721 cccaaagaca gcgccctgag acgtgcgctg acgcaggagc tcgggtcact gcgggcacgc
      781 cagatcctgg cagaaaactt tccccggggt tcagccacct cactgataga acaccgcgcc
      841 cgtacacatg acagtgaaaa cgtcatccat gatctggctg cagaactcat ccgtaagcag
      901 ggatatcacc tctgatgtac agcatacaca tcactccccg ctgccggcgt cctgcacggg
      961 gacaggtctt cctgaaaacg ctgcgcgtgg acggtgacag tttcagcttc accctgccgt
     1021 ccgcctgctt cacgctgcgt ccctccccca atccccgggt cattttcgcc agtgacagac
     1081 gggctgacgg ggaaatgagt ggcgttcgcc tggctctgcc cggagagaca ggcacgctga
     1141 ttacaacggc cacctggcag gtcaacgtgc cggagcggcc tccttttgat atacagcatg
     1201 tcattcactg gctcctgccc tcgcctctca cggcgctgat gt
//
LOCUS       DAZFLD010000503         1239 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.506, whole genome shotgun sequence.
ACCESSION   DAZFLD010000503 DAZFLD010000000
VERSION     DAZFLD010000503.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1239
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.506"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            353..>1239
                     /locus_tag="ACHKBL_005135"
     CDS             353..>1239
                     /locus_tag="ACHKBL_005135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001379687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HFO5821842.1"
                     /translation="MEARAEATSARSRVEQARSAHWPVVTATGSKLLSQSHRYDYDYD
                     SEDILPGIRGEMNLFASGAIEADVSRSESEAEYYRYKVDEAGEETIRSFVSLYLDALR
                     EKQSIAVLEQSLSRHNAILNDLNTISIHDTGRESEFVQAEARRLMVRQQINTRTRVLK
                     TTLGKLSTWTTNPVTEADLENPFSRMTESRLLTDFTQTPQKGNPSWLASQADVESKKA
                     ALKAQELARYPRVDLTGSVTRDDRQIGVSLSWDLFNRNASYGVSEKAAQIAAATGRLD
                     SVARMIDETGRLSLITVRQ"
BASE COUNT          307 a          291 c          309 g          332 t
ORIGIN      
        1 attaaatttg tttgttttaa aatttacctt gtgaaaattt tctccataaa ctgcaaggat
       61 tgtaagggtt tttcagcttt tcaataaaat tgaagtatct cacattttta ttatcttctg
      121 tcagcgcgga agataatggc ggtagcttgg atcaattccg gtgtctctgc atttcaattt
      181 tactcgatat tttacccggt tgcatcatga tgcctgatta tatttcccgc gtgtcccgga
      241 ggttcgggtg aagagaatat tatctttctg ttttcttttc tctgtccaca acgtgttcgc
      301 ttctccactg ccggccctcc tgagggatgc tctcattcat gatccatcag tgatggaggc
      361 aagggcagaa gccacctctg cccgttcccg tgtggagcag gctcgttcag cacactggcc
      421 ggtcgtcaca gcaacaggca gtaaactcct ttcccagagt caccgctacg actatgacta
      481 cgactctgaa gatattttgc ccggtatccg cggtgaaatg aatctgtttg cttcgggagc
      541 tattgaggcg gatgtcagcc ggagtgagtc ggaagccgag tattaccgtt ataaagttga
      601 tgaggctggc gaggaaacaa ttcgctcctt tgtttcatta tatcttgatg cgctaaggga
      661 aaagcaatcc attgcggtac ttgaacagag tctctcccgg cataacgcaa ttcttaatga
      721 tcttaacacc atcagtattc acgataccgg gcgggaatct gaatttgttc aggccgaagc
      781 ccgcaggctg atggtccggc agcagataaa tacccgcacc cgagtgctca aaaccacgct
      841 gggaaaactt tcgacctgga ccacaaatcc ggtaaccgaa gccgatcttg aaaatccttt
      901 ttcccggatg acagagtcca gattgttaac tgattttacg cagacgcctc agaaagggaa
      961 tccgtcgtgg cttgccagtc aggctgatgt agagagtaaa aaagcggcgc tgaaagcgca
     1021 ggagcttgcc cggtatcccc gggtggattt aacgggatca gtgacccggg atgacaggca
     1081 gataggtgtc agtctgtcct gggacctgtt caaccgtaat gccagttatg gtgtcagtga
     1141 aaaggccgcg cagatagctg cagctaccgg acgactggac tctgttgccc gaatgattga
     1201 tgaaaccgga cgattatcgc tgataacggt cagacaaag
//
LOCUS       DAZFLD010000504         1230 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.507, whole genome shotgun sequence.
ACCESSION   DAZFLD010000504 DAZFLD010000000
VERSION     DAZFLD010000504.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1230
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.507"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..161
                     /locus_tag="ACHKBL_005136"
     CDS             <1..161
                     /locus_tag="ACHKBL_005136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HFO5821843.1"
                     /translation="ASNKQLAAQQKAQKKQNIGTGIGTGAAIGASVGGPVGAVAGAVI
                     GGIAGSLF"
     gene            172..>1230
                     /locus_tag="ACHKBL_005137"
     CDS             172..>1230
                     /locus_tag="ACHKBL_005137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5821844.1"
                     /translation="MSGFAQGLLAGFSTVDQAMTRRKELGLREAQLAQQQKNNERDFE
                     FAQSQFEHNKDVDQRNFDYRAKVDDRNYTLQEREFNANQNYRNASLGMEQQRLQLQKY
                     NQRRLEYNDMLARDQPVMAALGKAIDAGDHDAASHLFGKLSDANPLKMMSTVGYAAKA
                     GQAVNNLQKIFDDKPDRAIASLNTPENLDVLSGVFAPELQQRIGMPDSTGEKTIKEAR
                     IGSIVPAQQEGYVLIGLDLTYSDGSTAHKPVTEYGSAHPDDQTVLAIPVDKAIAQVRD
                     RSKFAEISKNYGYFMPKQQGLSLAKLQEVASNVAADAIKNGRDAQAEVDEFYAANGLQ
                     PHQQKVQQRKTQQLLQKWQ"
BASE COUNT          338 a          268 c          351 g          273 t
ORIGIN      
        1 aggcatcaaa taaacaactg gctgctcaac agaaagctca gaaaaaacag aatatcggta
       61 cgggcattgg tacgggggcg gctattggcg catccgttgg tggtcctgtt ggcgctgttg
      121 ctggtgcagt aattggcggc attgctggtt ctttgtttta aggagtggtg aatgagcgga
      181 tttgcacagg ggttacttgc cggattcagc actgttgacc aggcaatgac ccgtcgtaag
      241 gagcttggtt tgcgagaagc acagcttgct cagcaacaga aaaataacga gcgcgatttt
      301 gaatttgcgc agtctcagtt tgaacataat aaagacgttg atcagcggaa ctttgattac
      361 agagccaaag ttgatgaccg caattacaca ctgcaggaaa gggagtttaa cgccaaccag
      421 aattaccgga atgcgtcgct ggggatggaa cagcaacgac tccagttgca gaaatacaac
      481 cagcgacggc ttgagtataa cgatatgctg gcgcgcgatc agcctgtgat ggcggcgctg
      541 ggaaaagcga ttgatgcggg tgaccatgat gcggcttctc atctgttcgg taaattatcg
      601 gacgccaatc cgcttaagat gatgtcaacg gtaggctatg ctgcgaaagc gggtcaggcc
      661 gtgaacaacc tgcagaaaat ctttgatgat aagccggaca gggcgattgc ttcgctgaat
      721 accccggaaa atctcgatgt gctttccggc gtgtttgccc cggaactaca acagcgtatt
      781 ggcatgcctg attcaaccgg ggagaaaacg ataaaagagg ccaggattgg cagtatcgta
      841 ccggcgcagc aggaagggta cgtacttatt ggccttgatc tcacatacag cgatggctcc
      901 accgcgcata aacctgtaac agaatacggc agtgcgcacc ctgatgatca aaccgtgctg
      961 gcgatacccg ttgataaggc tatcgctcag gtcagggatc gcagcaaatt tgcagagata
     1021 tcgaaaaatt atggttattt tatgccgaag cagcagggac tttctctggc gaaacttcag
     1081 gaagtagcca gcaacgtagc ggcggatgcg atcaagaatg gccgtgatgc tcaggctgag
     1141 gtggatgaat tttatgctgc gaatggttta cagccgcatc aacagaaagt tcagcaacgg
     1201 aaaacgcaac agcttttaca gaagtggcag
//
LOCUS       DAZFLD010000505         1228 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.508, whole genome shotgun sequence.
ACCESSION   DAZFLD010000505 DAZFLD010000000
VERSION     DAZFLD010000505.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1228
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.508"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1033
                     /locus_tag="ACHKBL_005138"
     CDS             <1..1033
                     /locus_tag="ACHKBL_005138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HFO5821845.1"
                     /translation="LFREKIGKLHLAQGMWELIDNSQLDDEMAEMKTTITETRNEITQ
                     TVSKTLEDQSATIQQIQRVQTDTNNDLAALYMLKVQKTKNGIPYVAGIGAGIEDADGQ
                     PLSNILLQADRIAMINPENGNTTPLFVAQGNQLFMNDVFLKRLFAVSITSSGNPPTFS
                     LTPEGRLTARNADISGHISANSGTLNNVTINQNCRILGKLSANQIEGDIVKTVGKAFP
                     RDSRAPERWPSGTITVRIYDDQPFDRQIVIPAVAFSGAKHEREHNDIYSSCRLMVKCN
                     GRVLFERTALAESQVYSGVIDMPAGRGHMTLEFSVSAWLVNGWYPTASISDLLVVVMK
                     KSTAGISIS"
BASE COUNT          315 a          290 c          372 g          251 t
ORIGIN      
        1 gctgtttcgg gaaaagatag gaaaactgca tctggcacag gggatgtggg agctgattga
       61 taacagccag cttgatgatg agatggcaga gatgaagacc accatcacag aaacccgcaa
      121 tgaaatcaca cagacggtca gtaaaaccct ggaagaccag agtgccacca tacagcagat
      181 acagcgggtg cagacagaca caaataacga cctggctgcg ctgtacatgc tgaaggtgca
      241 gaaaacgaaa aacggcattc cgtatgttgc cggtataggt gcggggattg aggatgctga
      301 tggccagccc ctgagcaata ttctgctgca ggcggaccgt atcgcgatga ttaacccgga
      361 gaacggcaac accacgccgc tgtttgtggc gcaggggaat cagctgttca tgaacgatgt
      421 gttcctgaag cgactgtttg cagtgagcat cacctcgtcc ggcaatcccc cgacgttctc
      481 cctgacgccg gagggcaggc tgacggcccg caatgcggac atcagcggac atatcagtgc
      541 gaactcgggc acgctcaata atgtgaccat taaccagaac tgccggatac ttggaaaact
      601 gtctgccaac cagattgaag gcgatattgt taaaaccgtg gggaaggctt tcccgcggga
      661 ctcccgcgca ccggagcggt ggccatcggg gaccattacc gtcaggattt atgacgatca
      721 gccttttgac cggcaaattg tgatcccggc agtggctttc agcggcgcta aacatgagag
      781 agagcataac gatatttatt catcatgccg gctgatggtg aaatgtaacg gcagggtgct
      841 ttttgaaagg acggcgctgg ctgagtctca ggtgtattca ggggtgattg atatgcctgc
      901 aggaaggggg catatgacgc tggagttttc tgtgtctgcc tggctggtga atggatggta
      961 cccgacagcg agtatcagcg atctgctggt tgtcgtgatg aagaagtcca cggcgggtat
     1021 cagtatttcg tgatcatgag gagccggatt gccggaagga gacaaaaacc gtacattatg
     1081 cgcgggtgcc tttggctgat ggccggaggg agcacctgaa ggccggatgt gaaaaggccc
     1141 cggacaaaca ttcatgttta acccgaggcc tgacgttcct accttcaaca agtggaaggt
     1201 tagcgcctct ccgtaaaagg agcaagcg
//
LOCUS       DAZFLD010000506         1221 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.509, whole genome shotgun sequence.
ACCESSION   DAZFLD010000506 DAZFLD010000000
VERSION     DAZFLD010000506.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1221
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.509"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1027
                     /locus_tag="ACHKBL_005139"
     CDS             <1..1027
                     /locus_tag="ACHKBL_005139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000514889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HFO5821846.1"
                     /translation="FFRGEIGKTHLAQGMWELIDNSQLDDEMAEMKTTITETRNEITQ
                     TVSKTLEDQSATIQQIQRVQADTNNDLAALYMLKVQKTKNGIPYVAGIGAGIEDADGQ
                     PLSNILLQADRIAMINPENGNTTPLFVAQGNQLFMNDVFLKRLFAVSITSSGNPPTFS
                     LTPEGRLTARNADISGHISANSGTLNNVVIAENCTINGTLKAENIIGDLVKCAGVAFP
                     VDGSYLANGTRTLTVYDDHSFDRQIIIPPIIYVGSKQESRTSNDIWTECFLHVDQNGR
                     RIYSGRSVTEPGIFSGIIDMPAGGGHITLSFTVSSRRQNGSFGSSRISNLQAIVVKKN
                     SAGISIR"
BASE COUNT          324 a          294 c          358 g          245 t
ORIGIN      
        1 ctttttcaga ggagaaatcg ggaagacaca tctggcacag gggatgtggg agctgattga
       61 taacagccag cttgatgatg agatggcgga gatgaagacc accatcaccg aaacccgcaa
      121 tgaaatcacg cagacggtca gtaaaaccct ggaagaccag agtgccacca tacagcagat
      181 acagcgggtg caggcagaca caaataacga cctggctgcg ctgtacatgc tgaaggtgca
      241 gaaaacaaaa aacggcattc cgtatgttgc cggtattggt gcggggattg aggatgctga
      301 tggccagccc ctgagcaata ttctgctgca ggcggaccgt atcgcgatga ttaacccgga
      361 gaacggcaac accacgccgc tgtttgtggc gcaggggaat cagctgttca tgaacgacgt
      421 gttcctgaag cgactgtttg cagtgagcat cacctcgtcc ggcaatcccc cgacgttctc
      481 cctgacgccg gagggcaggc tgacggcccg caatgctgac atcagcggac atatcagtgc
      541 gaactcgggc acgctgaata atgtcgtgat agcggagaac tgtacgataa atggcacgct
      601 gaaagcggag aacattattg gtgatcttgt gaaatgtgca ggggtggctt ttccggtgga
      661 tggtagttac cttgcgaacg gtacacgaac gctgacggtg tatgacgatc acagctttga
      721 ccggcagatt ataatcccgc cgataatcta tgtcgggtca aaacaggaat cccgcaccag
      781 taatgacatc tggacagagt gcttcctgca tgttgatcag aacggacgcc ggatttattc
      841 cggcaggtca gtgacagagc cgggaatttt cagcgggatc atcgatatgc cagccggagg
      901 tggtcatatc accctgagtt ttaccgtaag ttcacggcgt cagaacggga gttttggcag
      961 ttcacgtatc agtaaccttc aggcgatagt ggtgaagaaa aacagcgcgg ggatcagtat
     1021 ccgctgaaca tcgcgccccg ggattgccgg aaggatacaa aaaccgtaca ttatgtgcgg
     1081 gtgcctttgg ctgatggccg gagggaacac ctgaaggccg gaaagacgaa ggccccggac
     1141 aaacattcat gtttaacccg aggcctgacg ttcttccttc agcaagaaga aggttagcgc
     1201 ctctccgtaa aaggagcaag c
//
LOCUS       DAZFLD010000507         1220 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.510, whole genome shotgun sequence.
ACCESSION   DAZFLD010000507 DAZFLD010000000
VERSION     DAZFLD010000507.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1220
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.510"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(98..283)
                     /locus_tag="ACHKBL_005140"
     CDS             complement(98..283)
                     /locus_tag="ACHKBL_005140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5821847.1"
                     /translation="MLKTFRVFSRAVNKRGHTIGITQNVKAENVQAAISTVINKSAKA
                     GLSGVIIHAVTCIQDEE"
     gene            complement(276..740)
                     /locus_tag="ACHKBL_005141"
     CDS             complement(276..740)
                     /locus_tag="ACHKBL_005141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000916858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HFO5821848.1"
                     /translation="MDKNMLIASSFGLYDAGVAHMATRFDSLNTKADSRLHSDMRFFC
                     VRKPTHTRIMAGRNGGAFALAGFFGSSLSTLLRLATMFDSVVARLLIKTKGAFHMAVS
                     ARPYFVWRFMQCLTGSIAIFTVTAATEREARAQLPHAHLIFVARIRQEVTHA"
     gene            complement(733..921)
                     /locus_tag="ACHKBL_005142"
     CDS             complement(733..921)
                     /locus_tag="ACHKBL_005142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HFO5821849.1"
                     /translation="MATTPTLVHSQTAFIWRFIIFGASESQIIHVTAWTEREARNRCP
                     SGCVAVFAARIRQEASHG"
     gene            complement(952..1194)
                     /locus_tag="ACHKBL_005143"
                     /pseudo
     CDS             complement(952..1194)
                     /locus_tag="ACHKBL_005143"
                     /inference="COORDINATES: protein motif:HMM:NF022029.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ash family protein"
BASE COUNT          310 a          299 c          334 g          277 t
ORIGIN      
        1 cacctgtgtt acccatcatc agagaacaaa taagttctgc ctgtattgcg ctttttgctg
       61 cttcatcaac agtcatatcg gaaaaatcac acatgaatta ctcctcatcc tgaatacatg
      121 tcactgcgtg gataattacg ccggataaac cggctttagc tgatttgtta attactgtgc
      181 tgattgcagc ctggacgttt tcggccttca cgttctgcgt gatgccgatg gtatggccac
      241 gcttgttaac agcacgggaa aagacgcgga aggttttaag catgagtcac ctcctgacgg
      301 attcgggcga caaaaatcag atgtgcgtgc ggcagctgcg cgcgtgcttc gcgctcagtg
      361 gcagcggtaa cggtgaatat tgcaatgctg cctgtcaggc actgcataaa gcgccagaca
      421 aagtaagggc gtgcggatac agccatgtga aaggctcctt tggtttttat aaggagtctc
      481 gctactacgc tgtcaaacat ggtggcgaga cgtaacaggg ttgacagact ggaaccaaag
      541 aaaccagcga gcgcaaaggc tcccccgtta cgccccgcca taatgcgggt atgggtaggc
      601 ttacggacac aaaaaaaccg catatcggaa tgtaagcggc tgtccgcttt ggtattcagg
      661 ctgtcaaacc tggtcgccat gtgggcgact ccggcatcat acagcccgaa agaagaggcg
      721 atcaacatgt ttttatccat gtgatgcctc ctgacggata cgggcggcga atacagcaac
      781 gcaacctgac gggcaacggt tacgcgcttc gcgttccgtc caggcggtta cgtggatgat
      841 ttgggattct gacgcgccaa agatgataaa gcgccagata aaggcagtct gagaatgtac
      901 gagggtaggg gtagtagcca tgttggcagc ctccttacag tgatgaataa cgtcaccgca
      961 ggagttccta cgctcacaat gggcggtgac actggcgggg gtaggaatac cggactgtaa
     1021 ggactccggc ctacctttcg gtagccccgc cagcatcacc attgtttgct ggtggcgctg
     1081 tgcaaaatgc aactcgtgaa atgactggat ggtgcacgag tacgcacaat acacaacgcc
     1141 attgatatgg cgtaaagcgc tggcagaaaa aaagacgctt ctggcgtcat ttatcgcctt
     1201 acagttaaac gggttcctac
//
LOCUS       DAZFLD010000508         1220 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.511, whole genome shotgun sequence.
ACCESSION   DAZFLD010000508 DAZFLD010000000
VERSION     DAZFLD010000508.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1220
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.511"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            118..720
                     /gene="ais"
                     /locus_tag="ACHKBL_005144"
     CDS             118..720
                     /gene="ais"
                     /locus_tag="ACHKBL_005144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II)-phosphate
                     phosphatase Ais"
                     /protein_id="HFO5821850.1"
                     /translation="MLAFCRSSLKSKKYFIILLALAAIAGLGTHAAWSSNGLPRIDNK
                     TLARLAQQHPVVVLFRHAERCDRSTNQCLSDKTGITVKGTQDARELGNAFSADIPDFD
                     LYSSNTVRTIQSATWFSAGKKLTVDKRLLQCGNEIYSAIKDLQRKAPDKNIVIFTHNH
                     CLTYIAKDKRDATFKPDYLDGLVMHVEKGKVYLDGEFVNH"
     gene            complement(759..1184)
                     /gene="nudI"
                     /locus_tag="ACHKBL_005145"
     CDS             complement(759..1184)
                     /gene="nudI"
                     /locus_tag="ACHKBL_005145"
                     /EC_number="3.6.1.12"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HFO5821851.1"
                     /translation="MRQRTIVCPLIQNDGAYLLCKMADDRGVFPGQWALSGGGVEPGE
                     RIEEALRREIREELGEQLLLTEITPWTFSDDIRTKTYADGRKEEIYMIYLIFDCVSAN
                     REVKINEEFQDYAWVKPEDLAHYDLNVATRKTLRLKGLL"
BASE COUNT          325 a          280 c          265 g          350 t
ORIGIN      
        1 ggcattttgc cggcatcgta ggatttttaa tattacacca actgctaatt atcgccagaa
       61 atgtcgctca atttcacggt aattgtctgg ttgcgcttgt ctataggtgg agtttacgtg
      121 ttagcttttt gccgctcttc gttgaagtca aaaaaatatt tcatcatttt actggcgctc
      181 gctgcaattg ccggactggg tactcatgcc gcctggagta gcaatggttt gccacgtatc
      241 gacaataaaa cgctggccag actggcacag cagcatccgg ttgtcgtttt gtttcgccat
      301 gctgaacgtt gcgatcgttc aaccaatcaa tgcttgtcag ataaaacagg tattacggtc
      361 aaaggtaccc aggatgcccg tgaactgggc aacgctttta gtgctgatat ccctgatttc
      421 gatctctatt ccagtaatac cgtccggacc attcagtcgg cgacctggtt ttcagcgggt
      481 aaaaagctga cggtagataa acgacttctt caatgcggta atgagattta tagtgcaatt
      541 aaagacttac aacgcaaagc gcctgataaa aatatcgtta tttttaccca taatcattgc
      601 ctgacatata ttgctaaaga taagcgtgat gcgacattta aacctgatta tctggatggt
      661 ttagtcatgc atgtggaaaa aggcaaagtt tatctggatg gggaattcgt taaccactaa
      721 attttcaatc tgacagccag tgatggctgt catcattatt acagaagacc tttcaaacgt
      781 aacgtttttc gggtggcgac attcaaatca tagtgcgcca gatcttcagg ttttacccac
      841 gcgtagtctt gaaactcttc gtttattttc acttctcggt tggcagaaac gcagtcaaaa
      901 atcaggtaaa tcatataaat ctcttccttg cgaccatctg catacgtctt ggtgcgaata
      961 tcatcgctga aggtccacgg cgtgatttct gtcaaaagca gctgttctcc cagctcttcg
     1021 cgaatttcgc ggcgtagtgc ctcttcaatt cgttcgccgg gctccacgcc gccacccgaa
     1081 agcgcccatt gaccggggaa aacgccgcga tcgtcggcca ttttacacag caaataagca
     1141 ccatcatttt gaatcaaagg gcatacaata gtccgttgtc gcacaggggt attccttata
     1201 aataaaaaga aaccatgaga
//
LOCUS       DAZFLD010000509         1216 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.512, whole genome shotgun sequence.
ACCESSION   DAZFLD010000509 DAZFLD010000000
VERSION     DAZFLD010000509.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1216)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1216)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1216
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.512"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(46..279)
                     /locus_tag="ACHKBL_005146"
     CDS             complement(46..279)
                     /locus_tag="ACHKBL_005146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HFO5821852.1"
                     /translation="MPGCTARLLPEGPFSRGQAVAVTTAYRNVFTEDDQGTYFRLVIR
                     NAEGQLRWRCWNFESDAGKQLNVWLSSEGLLRQ"
     gene            complement(350..>1216)
                     /locus_tag="ACHKBL_005147"
     CDS             complement(350..>1216)
                     /locus_tag="ACHKBL_005147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HFO5821853.1"
                     /translation="HLGHDNNGGIARGATPESSGSYGFVRLEGDLLITEVAGMSLTTG
                     VYGAAGHSSVDVKDDDGSRAGTVRDDAGSLGGYMNLTHTSSGLWADIVAQGTRHSMKA
                     SSGNNDFRARGWGWLGSLETGLPFSITDNLMLEPQLQYTWQGLSLDDGQDNAGYVKFG
                     HGSAQHVRAGFRLGSHNDMNFGKGTSSRAPLRDSAKHSVRELPVNWWVQPSVIRTFSS
                     RGDMRVGTSTAGSGMTFSPSQNGTSLDLQAGLEARVRENITLGVQAGYAHSVSGSSAE
                     GYNGQATLNVTF"
BASE COUNT          257 a          361 c          346 g          252 t
ORIGIN      
        1 gcgtctcccg gagaagatgc ggcctgatgg atgaaatgaa gacgtttatt gcctgagaag
       61 cccttcactg gagagccaca cattaagctg ttttccggcg tcagactcaa aattccagca
      121 ccgccagcgt agctgccctt cggcattacg gataaccagc cggaaatatg ttccctggtc
      181 atcttcagta aacacattgc ggtaagctgt tgtgacggca acagcctgtc cgcgtgagaa
      241 agggccttcg ggcaataaac gtgctgtaca acccggcata tctgttgctc cctgaaagta
      301 aaaagccccg gtcatgatga ccggggcctg aaggggagtg acctgattat cagaaagtca
      361 cattcagcgt ggcctgaccg ttatagcctt cagcgctgct gccgctgacg ctgtgggcat
      421 aaccggcctg aacgcccagg gtgatatttt cccggacacg ggcttccagt ccggcctgca
      481 ggtccagtga cgtgccattc tgggacggtg agaacgtcat cccgctgcct gcggtggagg
      541 tccccacacg catatcaccc cgggagctga aggtgcggat aacggaaggc tgtacccacc
      601 agttcaccgg cagttcacgc acactgtgtt ttgcactgtc acgcaggggg gcacgggatg
      661 aggtgccttt accaaagttc atatcgttgt ggctgcccag acggaagccg gcacgcacat
      721 gttgtgcact gccatgcccg aacttcacat aaccggcgtt atcctggccg tcatccaggg
      781 aaagtccctg ccaggtgtac tgcagttgtg gctccagcat caggttgtca gtgatactga
      841 agggcagacc ggtttccagt gagcccagcc agccccagcc ccgtgcgcgg aagtcgttat
      901 tgcccgatga cgctttcata ctgtggcggg ttccctgtgc cactatgtca gcccacaggc
      961 cggaggaggt gtgtgtcagg ttcatatatc cgcccaggct gccggcatca tcccggaccg
     1021 tgccggcgcg ggagccgtca tcatccttaa catcaacgga agaatggcct gcagcaccat
     1081 acaccccagt cgtcagtgac ataccggcga cctctgttat gagcaggtca ccctccagac
     1141 ggacgaagcc atagctgccg ctgctttccg gcgtggcccc acgggcaata ccgccgttgt
     1201 tatcgtgacc gagatg
//
LOCUS       DAZFLD010000510         1215 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.513, whole genome shotgun sequence.
ACCESSION   DAZFLD010000510 DAZFLD010000000
VERSION     DAZFLD010000510.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1215
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.513"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(85..225)
                     /locus_tag="ACHKBL_005148"
     CDS             complement(85..225)
                     /locus_tag="ACHKBL_005148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFO5821854.1"
                     /translation="MPQKTIIVSILCLTMLLTVWMIHRSPCEFRLNIMWSEFAAFLQC
                     KP"
     gene            complement(543..683)
                     /locus_tag="ACHKBL_005149"
     CDS             complement(543..683)
                     /locus_tag="ACHKBL_005149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFO5821855.1"
                     /translation="MPQKTIIVSILCITILMIVWMIHKSPCEFRLNIMWSQFAAFLQC
                     KQ"
     gene            complement(793..1128)
                     /locus_tag="ACHKBL_005150"
     CDS             complement(793..1128)
                     /locus_tag="ACHKBL_005150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5821856.1"
                     /translation="MQGFAKVCKNSGFLRIPKNFNALNNKRFLKLRHDRRSEKGSGDE
                     NGKIRKTRTGAAWRGVAAKITFAHFCTTAPKKDGEGEGKQPDHPLHVTDVKGKLRAGA
                     FRLTAGRNT"
BASE COUNT          279 a          311 c          286 g          339 t
ORIGIN      
        1 tttgtgcctt ttgtgtgagg gtgcgacttt cgaccgaacc tttggcaacc ggatagcggg
       61 ggctttcacc cccgctgcgg tttcttacgg tttacactgc aagaacgccg caaattctga
      121 ccacatgatg ttaagtctga actcacacgg agaacggtgt atcatccaga cagtcaacaa
      181 catggttaag cataaaatgc tcacgatgat cgttttttgc ggcatagcgc ttgactcctt
      241 ttacagggag gcgctaacct tccacttgct tagggtagat ggtcagggcc tcgggttaac
      301 attaaagttg actccggggc ctttccacat ccggccttca ggtaatcctt ccagccatca
      361 gccgaaaggc acccgcgtgt aatgtactga tttccctttg tgtgcgcaat gcttgagggg
      421 gacggtaaaa aaacgcccgc atattgcggg caaatacgtc tgtaatccaa tggagaaagg
      481 ggtgccatat cgaccgaacc cggcaaccgg atagcggggg ctttcacccc cgctgcggtt
      541 tcttactgtt tacactgcaa gaacgccgca aattgtgacc acatgatgtt aagcctgaac
      601 tcacacggtg acttgtgtat catccagaca atcatcaaaa tagttatgca taaaatgctc
      661 actatgatcg ttttttgcgg catagcgctt gactcctttt acagggaggc gctaaccttc
      721 cacttgtcag gtaggaagtg tcagggcctc gggttaacgt tagttgactc ggggcctttt
      781 cacatccggc cttcaggtat tccttccagc cgtcagccga aaggcacccg cgcgtaattt
      841 accttttacg tccgttacgt gcaacggatg atccggctgt tttccttccc cttcgccatc
      901 ctttttcgga gcagttgtgc aaaagtgtgc aaaagtgatt tttgctgcca ctccccgcca
      961 ggccgcgcca gttctggttt tcctgatttt tccgttttcg tcaccggatc ctttttcaga
     1021 tcttcgatcg tgacgtaatt ttaaaaatct cttgttattc agtgcattaa aatttttcgg
     1081 gatcctcaga aagccggagt ttttgcacac ttttgcaaaa ccttgcacaa agtgcaaaac
     1141 cattcagaag ggaaaaactc agtaatggcg cgccctggag gggtgttttg cacccctttc
     1201 gatttgcaaa attta
//
LOCUS       DAZFLD010000511         1213 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.514, whole genome shotgun sequence.
ACCESSION   DAZFLD010000511 DAZFLD010000000
VERSION     DAZFLD010000511.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1213
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.514"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..651)
                     /locus_tag="ACHKBL_005151"
     CDS             complement(<1..651)
                     /locus_tag="ACHKBL_005151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaB-like helicase N-terminal domain-containing
                     protein"
                     /protein_id="HFO5821857.1"
                     /translation="MAEMSVLCAYEAEQAVLGGLMLENERWDEVVLMLSLDDFYVAQH
                     RAIFRAMSELAVNGQPLDLITLSEHLEHQGRLDVLGGFAYLAELSKNTPSAANIMAYA
                     GIVVEKSVLRQLQATGNTLIADVAAPNATSRDVLDAAERRLFALAQNRTLRERTEASL
                     SSALDTVLQQLESVLGSGGVTGTPTGFDCLDEMTCGLQPGDLVLLAARPSMGKTSLA"
     gene            848..>1213
                     /locus_tag="ACHKBL_005152"
     CDS             848..>1213
                     /locus_tag="ACHKBL_005152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5821858.1"
                     /translation="MNTEKQLPSGIESEVKYTLQFWLLSNGKVMTEKDGLCNPFPLNC
                     IESAHPFTPFQQNQWVELDTVGPNGPVKATFLVHSVLHKLKRQGGSLNDGNWPEYGRC
                     IAHWHTDVFVEKKALPDWLR"
BASE COUNT          304 a          334 c          317 g          258 t
ORIGIN      
        1 cgccagagag gttttcccca ttgacggacg ggcagccagc agaaccaggt caccaggctg
       61 caggccacag gtcatttcat ccagacagtc aaatcccgtg ggggtaccgg taacaccacc
      121 ggagccgagc acactttcca gttgctgcag aacggtatca agcgcggatg aaagagaggc
      181 ttccgtgcgt tcccggagcg tcctgttctg cgcaagcgca aacagacgcc gctctgcagc
      241 atccagcaca tcccgtgatg tggcgttcgg tgccgccaca tcagcaatca gggtgtttcc
      301 ggtggcctgt aactgacgca gaacgctttt ctccaccaca atgcctgcat aggccatgat
      361 gttggccgca gaaggtgtgt tcttgctgag ctcggccaga taagcaaagc cgcccagtac
      421 gtccagcctg ccctgatgtt ccaggtgctc tgacagtgta atcaggtcca gtggctggcc
      481 attcaccgca agttcagaca tggcacggaa aatggcccgg tgctgagcca cataaaaatc
      541 gtcaagactc agcatcagca ccacttcatc ccagcgttca ttctccagca tcagaccgcc
      601 cagaacggcc tgctctgctt cgtatgcgca gagaacagac atttcagcca tggcgtaccc
      661 tccctctctg actgccggaa acctggctac gtgaaaaaag gaaaaagggt gccggacgta
      721 cagctgtcgg gaataaaatc ccggtaactg tctgtacgtt ttcagaccag taagtctggc
      781 aaccccggtc tgaaagtaca gacagcattc gttcattcat aacaagttgt tttaaaggaa
      841 aaagtttatg aatacagaaa aacaactgcc gtccggcatt gaatctgagg tgaaatacac
      901 cctgcagttc tggctgctga gtaacggaaa ggtcatgacg gaaaaagacg ggctctgtaa
      961 cccctttccc ctgaactgta ttgaatcggc tcatcctttt actccgtttc agcagaatca
     1021 gtgggttgaa ctggacactg tcgggccgaa tggaccggtg aaagcgacat tccttgtgca
     1081 cagcgttctg cataaactga aacgtcaggg tggctccctg aatgatggca actggcctga
     1141 atacggccgg tgcattgctc actggcacac tgacgtgttt gttgagaaaa aagcgctgcc
     1201 tgactggctg aga
//
LOCUS       DAZFLD010000512         1210 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.515, whole genome shotgun sequence.
ACCESSION   DAZFLD010000512 DAZFLD010000000
VERSION     DAZFLD010000512.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1210
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.515"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            31..903
                     /locus_tag="ACHKBL_005153"
     CDS             31..903
                     /locus_tag="ACHKBL_005153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HFO5821859.1"
                     /translation="MNPSDAIEAIEKPLSSLPYSLSRHILEHLRKLTRHEPVIGIMGK
                     SGAGKSSLCNALFQGEVTPVSDVHAGTREVRRFRLSGHGHSMVITDLPGVGESRDRDA
                     EYEALYRDILPELDLVLWLIKADDRALSVDEYFWRHILHRGHQQVLFVVTQADKTEPC
                     HEWDMAGIQPSPAQAQNIREKTEAVFRLFRPVHPVVAVSARTGWELDTLVSALMTALP
                     DHAASPLMTRLQDELRTESVRSQAREQFTGAVDRIFDTAESVCVASVVRTALRAVRNT
                     VVSVARAVWNWIFF"
BASE COUNT          256 a          338 c          320 g          296 t
ORIGIN      
        1 gttttgtctt ttttcaccgg gaatacatgt atgaatccgt ctgatgccat tgaggcaatt
       61 gaaaaaccgc tctcctccct gccttactca ctttcccgtc acatcctgga acatctgcgc
      121 aaactcaccc gtcacgaacc cgtgattggc attatgggta aaagcggggc cggtaaatcc
      181 tcactctgta atgcactgtt tcagggggag gtcaccccgg tcagtgatgt tcacgccggc
      241 acccgggaag tacggcgctt ccgtctgagt ggccacggtc acagcatggt tatcactgac
      301 ctgcccgggg tgggcgagag ccgggacagg gatgcagagt atgaggccct gtaccgtgac
      361 attctgcctg aacttgacct ggtgctgtgg ctgattaaag ccgatgaccg ggccctgtct
      421 gtggatgagt atttctggcg acacatcctg caccggggac atcagcaggt gctgtttgtg
      481 gtgacgcagg ccgacaaaac ggagccctgc catgaatggg atatggccgg tattcagcct
      541 tctcctgcac aggcacagaa tattcgcgaa aaaacggagg cggtattccg tctgttccgg
      601 cccgtacatc cggttgtggc cgtatcggcc cgcaccggct gggaactgga tacgctggtc
      661 agtgcgctca tgacagcgct tcccgaccat gccgccagtc ccctgatgac ccgactgcag
      721 gacgagctgc gcacggaatc tgtccggtct caggcccgtg aacagtttac cggtgcggtg
      781 gaccggatat ttgacacagc ggagagcgtc tgtgttgcct ctgttgtccg tacggccctg
      841 cgcgctgttc gtaacacggt ggtctctgtt gcccgtgcgg tatggaactg gattttcttc
      901 tgaacctgtc gtgactgatg ccctccctga ctctgagtct gctcacaaaa gcgctgtttt
      961 cgttactgtc tctcttgtcc gtgcaatagc caaacaatag aataaaacga tcgataccta
     1021 ttttatcgat cgtttatatc gctcgataag ctaataataa cctttgtcag taacatgcac
     1081 agatacgtac agaaagacat tcagggaaca acagagccac acgtcagaaa cttccgtcag
     1141 ccggacctct ggcactgtaa ccctttacct gccggtatcc acatctgtgg ataccggctt
     1201 ttttattcac
//
LOCUS       DAZFLD010000513         1210 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.516, whole genome shotgun sequence.
ACCESSION   DAZFLD010000513 DAZFLD010000000
VERSION     DAZFLD010000513.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1210
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.516"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(419..1051)
                     /locus_tag="ACHKBL_005154"
     CDS             complement(419..1051)
                     /locus_tag="ACHKBL_005154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HFO5821860.1"
                     /translation="MKRLIMATMVTAILASSTVWAADNAPVAAQQQTQQVQQTQKTAA
                     AERISEQGLYAMRDVQVARLALFHGDPEKAKELTNEASALLSDDSTEWAKFAKPGKKT
                     NLNDDQYIVINASVGISESYVATPEKEAAIKIANEKMAKGDKKGAMEELRLAGVGVME
                     NQYLMPLKQTRNALADAQKLLDKKQYYEANLALKGAEDGIIVDSEALFVN"
BASE COUNT          318 a          257 c          251 g          384 t
ORIGIN      
        1 tgcactcgat atttttaaat tcctgtatca agtgctgata atcatttcat cgccacttcc
       61 aattcctttc tgcatggtaa gtagtgagga cgaaaagaag aatatgataa atattggttc
      121 gcggtaatca gagagggccg cgacatttgt gatgttattc ttacaactgt aagaagcgcc
      181 ggtgatgtac aagtaagggt atgattacgc tttttaaagc gtaccataaa acataacata
      241 ataaatgatt tatgatgaca cgggattatt gcggtttact ttgaagaata taaaatgcga
      301 gtttatagca ccgcgagcgc caaataattt tatggccaca acctgtcgta ttgtggccat
      361 aaaaaattgt cggctggagg gagtaaaatc ctttacctca atatattgcc tgtcagcttt
      421 agttcacaaa cagtgcttcg ctatcgacga tgattccatc ttccgcacct ttaagcgcca
      481 ggtttgcctc gtagtattgc tttttatcaa gcagtttctg cgcatcagca agtgcattac
      541 gagtctgttt gagcggcatc agatattggt tttccattac accaacaccg gccaggcgaa
      601 gctcttccat tgcgcccttt ttatcccctt tcgccatttt ttcgttggca atttttatcg
      661 cggcttcttt ttcgggtgtc gctacataac tctcagaaat tccgacagat gcattaatga
      721 caatatattg gtcatcattg agattggtct ttttacctgg cttagcgaac tttgcccatt
      781 cggtactgtc atctgacaac aaagctgaag cttcattggt cagttctttt gccttttccg
      841 gatcgccatg aaacagagcc aggcgcgcta cctgcacgtc acgcatcgca tataaacctt
      901 gttcagagat acgctctgct gctgccgttt tctgggtttg ctgaacttgc tgagtttgct
      961 gttgtgccgc taccggtgca ttatcagcag cccatacagt tgaagatgcc agaattgctg
     1021 tgaccatcgt agccataatt aaacgtttca tacttacctc attctttaac agttttagaa
     1081 aacgccgcca tcatattggg tgagcatgaa atgagaaatc agagagcaac ggtcattttg
     1141 aaagggttat cttacagttg tagttattat cgcaagaagt attttagtct tacaattgta
     1201 ggaatatgct
//
LOCUS       DAZFLD010000514         1208 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.517, whole genome shotgun sequence.
ACCESSION   DAZFLD010000514 DAZFLD010000000
VERSION     DAZFLD010000514.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1208
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.517"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            103..861
                     /locus_tag="ACHKBL_005155"
     CDS             103..861
                     /locus_tag="ACHKBL_005155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiY family protein"
                     /protein_id="HFO5821861.1"
                     /translation="MKLLKTVPAIVMLAGGMFASLNAAADDSVFTVMDDPASAKKPFE
                     GNLNAGYLAQSGNTKSSSLTADTTMTWYGQTTAWSLWGNASNTSSNDERSSEKYAAGG
                     RSRFNLTDYDYLFGQASWLTDRYNGYRERDVLTAGYGRQFLNGPVHSFRFEFGPGVRY
                     DKYTDNASETQPLGYASGAYAWQLTDNAKFTQGVSVFGAEDTTLNSESALNVAINEHF
                     GLKVAYNVTWNSEPPESAPEHTDRRTTLSLGYSM"
     gene            complement(914..1021)
                     /gene="yniD"
                     /locus_tag="ACHKBL_005156"
     CDS             complement(914..1021)
                     /gene="yniD"
                     /locus_tag="ACHKBL_005156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YniD"
                     /protein_id="HFO5821862.1"
                     /translation="MPTKRFDKKHWKMVVVLLAICGAMLLLRWAAMIWG"
BASE COUNT          302 a          272 c          289 g          345 t
ORIGIN      
        1 tttttataat tcgttacctt tccagggacg ctaatctttg atccttaaga aagtttccgg
       61 gaccgcgaag gtctcttttt ttgttgtacg gactcttgat aaatgaagct tttgaagaca
      121 gttcctgcca tcgttatgtt ggcggggggc atgtttgcgt cactgaatgc agctgccgat
      181 gattccgttt ttactgtcat ggacgatcct gccagcgcta aaaaaccttt cgaaggtaat
      241 ctgaacgcag gatatcttgc acaatcaggc aatacgaaaa gctcctcact tacggctgac
      301 accaccatga cctggtatgg acaaaccact gcctggtcgc tgtgggggaa cgccagtaat
      361 acttcttcta atgatgagcg ttcttcagaa aaatatgcgg cgggcgggcg tagccgtttt
      421 aatttaactg attatgacta tttatttggg caggcaagct ggctgacaga ccgttataac
      481 ggctatcgag agcgcgatgt gttgaccgcg ggttatggtc gccagttttt gaatggcccg
      541 gtacacagtt tccgctttga attcggtcca ggtgtgcgtt acgacaaata taccgataat
      601 gccagtgaaa cccagccgct gggttatgcc tccggggctt atgcatggca gttgactgac
      661 aacgctaaat ttacgcaggg tgtttccgtc tttggcgcgg aagacacaac gctgaactct
      721 gaaagcgcat taaatgttgc tattaatgaa cattttggac tgaaggtggc ttacaacgta
      781 acatggaact ctgaacctcc agagtctgca ccggaacata ccgatcgtcg gacaacctta
      841 tcgctgggct acagcatgta aaattatctg ccggaccgtg ttatgcggtc tggctgtctt
      901 cgtttgtcac tgctcagccc caaatcattg ctgcccaacg tagcaacaac atagcgccac
      961 agattgccag tagcaccacc accatcttcc agtgtttttt atcaaagcgt ttagtcggca
     1021 taccagtgtc ctgcatatct cttgtgcgtg ataacggagt gtaacaaaag ggacaggcat
     1081 caagggattt tttgttatta cagccgtcgg gtggcgttgt acacctgatc actgtttttg
     1141 cggtttatga aaaaaataaa attatgtatc gatatataaa tatctggtta taaatacgtc
     1201 tttagttc
//
LOCUS       DAZFLD010000515         1207 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.518, whole genome shotgun sequence.
ACCESSION   DAZFLD010000515 DAZFLD010000000
VERSION     DAZFLD010000515.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1207
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.518"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..938
                     /gene="ompT"
                     /locus_tag="ACHKBL_005157"
     CDS             <1..938
                     /gene="ompT"
                     /locus_tag="ACHKBL_005157"
                     /EC_number="3.4.23.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="omptin family outer membrane protease OmpT"
                     /protein_id="HFO5821863.1"
                     /translation="GIVLTTPIAISSFASTETLSFTPDNINADISLGTLSGKTKERVY
                     LAEEGGRKVSQLDWKFNNAAIIKGAINWDLMPQISIGAAGWTTLGSRGGNMVDQDWMD
                     SSNPGTWTDESRHPDTQLNYANEFDLNIKGWFLNEPNYRLGLMAGYQESRYSFTARGG
                     SYIYSSEEGFRDDIGSFPNGERAIGYKQRFKMPYIGLTGSYRYEDFELGGTFKYSGWV
                     EASDNDEHYDPGKRITYRSKVKDQNYYSVAVNAGYYVTPNAKVYIEGAWNRVTNKKGD
                     TSLYDHNDNTSDYSKNGAGIENYNFITTAGLKYTF"
BASE COUNT          386 a          252 c          253 g          316 t
ORIGIN      
        1 tgggaatagt cctgacaacc cctattgcga tcagctcttt tgcttctacc gagactttat
       61 cgtttactcc tgacaacata aatgcggaca ttagtcttgg aactctgagc ggaaaaacaa
      121 aagagcgtgt ttatctagcc gaagaaggag gccgaaaagt cagtcaactt gactggaaat
      181 tcaataacgc tgcaattatt aaaggtgcaa ttaattggga tttgatgccc cagatatcta
      241 tcggggctgc tggctggaca acactcggca gccgaggtgg caatatggtc gatcaggact
      301 ggatggattc cagtaacccc ggaacctgga cggatgaaag tagacaccct gatacacaac
      361 tcaattatgc caacgaattt gatctgaata tcaaaggctg gttcctcaac gaacccaatt
      421 accgcctggg actcatggcc ggatatcagg aaagccgtta tagctttaca gccagaggtg
      481 gttcctatat ctacagttct gaggagggat tcagagatga tatcggctcc ttcccgaatg
      541 gagaaagagc aatcggctac aaacaacgtt ttaaaatgcc ctacattggc ttgactggaa
      601 gttatcgtta tgaagatttt gagctaggtg gcacatttaa atacagcggc tgggtggaag
      661 catctgataa cgatgaacac tatgacccag gaaaaagaat cacttatcgc agtaaagtca
      721 aagaccaaaa ttactattct gttgcagtca atgcaggtta ttacgtaacg cctaatgcaa
      781 aagtttatat tgaaggcgca tggaatcggg ttacgaataa aaaaggtgat acttcacttt
      841 atgatcacaa tgataacact tctgactaca gcaaaaatgg tgcaggcata gaaaactata
      901 acttcatcac tactgctggt cttaagtaca ccttttaaca acgttaaata gattttctcc
      961 ccggtggtat tgtatagccc cggggaattt gtttaccgtt aaaagcaaac aatatcccct
     1021 aatcttgtaa gcatttaacc tgcgtcctgg cgtgtaacct cttaccgcaa cacaagactc
     1081 aatatattca aattcgttat gccgtggcat catttaaccc cttgtaattc atcgctataa
     1141 ttgatttaat tcacaaataa aactataaca tggtgaaact aatgaaaaaa aacacagatg
     1201 atggggc
//
LOCUS       DAZFLD010000516         1206 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.519, whole genome shotgun sequence.
ACCESSION   DAZFLD010000516 DAZFLD010000000
VERSION     DAZFLD010000516.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1206)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1206
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.519"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..635)
                     /locus_tag="ACHKBL_005158"
     CDS             complement(<1..635)
                     /locus_tag="ACHKBL_005158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000364797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFO5821864.1"
                     /translation="MDLLAESITEVAVSGEIANTDRVLNIAYGIDRNFLFGAAVSMQS
                     VVMHNPDLAVKFHLFTDYIDEDYLQRVNAFTSKNANVEVKIYKVSSAFIDIFPSLKQW
                     SYATFFRLVAFQYLSEVIENLLYIDADVICKGSLAGLLDINFDGDKFAAVIKDVPFMQ
                     EKPAKRLAIEGLPGNYFNAGVVYLQLEAWAKNDFMNKAIAMLASDPQHTKY"
     gene            complement(661..>1206)
                     /gene="rfaY"
                     /locus_tag="ACHKBL_005159"
     CDS             complement(661..>1206)
                     /gene="rfaY"
                     /locus_tag="ACHKBL_005159"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II) kinase RfaY"
                     /protein_id="HFO5821865.1"
                     /translation="IDTKYGRYVFKVFAPKTKRNERFFKSFVKGDYYQNLIVETDRVR
                     SAGLTFPNDFYFLAERKIFNYASVFIMLIEYVEGVELNDMPIIPENVKAEIKASMEKL
                     HALNMLSGDPHRGNFIVSKDGVRIIDLSGKSCTAERKARDRLAMERHLGIANEIKDYG
                     YYSVIYRTKLRKFIKKLKGKA"
BASE COUNT          373 a          251 c          216 g          366 t
ORIGIN      
        1 ttatatttcg tgtgctgcgg gtcacttgcc agcatagcaa tggctttatt cataaaatca
       61 tttttcgccc atgcttcaag ctgcagatat actacaccgg cgttgaaata attacctgga
      121 agtccctcta tagccagacg cttcgctggt ttttcctgca taaaaggcac atctttaata
      181 acagctgcga acttatcgcc atcaaaatta atatcaagca atccagctaa tgagccttta
      241 cagatgacat cagcatcgat atataacaga ttttcaataa cttcactcag atactggaac
      301 gcaactaaac ggaagaatgt tgcataagac cactgtttca ggctggggaa gatatcaata
      361 aaggcactgg agactttata aatttttact tcaacgttag catttttgct ggtaaaagcg
      421 ttaacacgtt gtagataatc ttcatcaatg tagtcggtaa agagatgaaa cttaaccgca
      481 aggtccgggt tatgcataac aactgattgc atagatactg ccgcaccaaa taaaaaatta
      541 cggtcaatac cgtaagcgat atttaacaca cgatcggtgt tagcaatttc cccagagaca
      601 gcgacttcag taatactctc agctaataaa tccatgttgg ttccgttttg actgtgtggt
      661 ttacgctttg ccttttaatt ttttaataaa tttgcgtagt ttggttctgt agattactga
      721 atagtagcca taatctttta tttcgttggc aatacccagg tgacgttcca tggccagacg
      781 atcacgagct ttacgctctg ccgtacagct tttaccggat aggtcaatta tcctgacacc
      841 atctttacta acgataaagt taccccggtg agggtcgccc gatagcatat ttaaggcatg
      901 caatttttcc attgaagcct taatttctgc tttaacattt tctggaataa tcggcatatc
      961 attgagttct acgccctcga catactcgat aagcatgata aaaacactgg catagttgaa
     1021 aattttacgc tcagccagaa aataaaaatc attaggaaaa gtaagacctg cacttctgac
     1081 acgatcggtt tcaacgatca aattctgata gtagtcacct tttacaaaag atttaaaaaa
     1141 acgttcattc cttttcgttt tgggagcaaa gactttaaaa acatagcgac catatttcgt
     1201 atcaat
//
LOCUS       DAZFLD010000517         1198 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.520, whole genome shotgun sequence.
ACCESSION   DAZFLD010000517 DAZFLD010000000
VERSION     DAZFLD010000517.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1198
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.520"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(45..932)
                     /gene="rhtA"
                     /locus_tag="ACHKBL_005160"
     CDS             complement(45..932)
                     /gene="rhtA"
                     /locus_tag="ACHKBL_005160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/homoserine exporter RhtA"
                     /protein_id="HFO5821866.1"
                     /translation="MPGSLRKMPVWLPIVILLVAMASIQGGASLAKSLFPLVGAPGVT
                     ALRLALGTLILIAFFKPWRLRFAKEQRLPLLFYGVSLGGMNYLFYLSIQTVPLGIAVA
                     LEFTGPLAVALFSSRRPVDFVWVVLAVLGLWFLLPLGQDVSHVDLTGCALALGAGACW
                     AIYILSGQRAGAEHGPATVAIGSLIAALIFVPIGALQAGEALWHWSVIPLGLAVAILS
                     TALPYSLEMIALTRLPTRTFGTLMSMEPALAAVSGMIFLGETLTPIQLLALGAIIAAS
                     MGSTLTVRKESKIKELDIN"
BASE COUNT          355 a          307 c          286 g          250 t
ORIGIN      
        1 gataaattct gcatggttat gcataaccat gcagaaatgt aaatttaatt aatgtctaat
       61 tcttttattt tgctctcttt gcgtactgtc agcgtagaac ccattgaagc ggcgataatt
      121 gcgccgagcg ccagcagctg tatgggtgtc agtgtttctc cgaggaaaat catcccggaa
      181 acggcagcca gtgccggttc catgctcatc agcgtaccaa atgtccgcgt tggcaaacgg
      241 gtgagggcaa tcatttccag cgaataaggc agagcggtcg agagaatagc gaccgccaga
      301 cccaatggaa taaccgacca gtgccagagt gcttcaccag cctgaagcgc tccaattggc
      361 acgaaaatta acgctgcaat caacgaacca attgccaccg tcgcagggcc atgttccgct
      421 cctgcgcgtt gcccacttaa aatgtaaata gcccaacaag cccctgctcc cagtgccagc
      481 gcacagccgg ttaaatcgac atgggaaacg tcttgcccca gcggtagcag gaaccacaga
      541 ccaagaaccg ccagaacaac ccagacgaaa tctaccgggc gacgagaaga gaacagcgcc
      601 accgccagtg gtccggtgaa ctccagcgcc accgcaatac ccagcggtac cgtctgaata
      661 gaaagataaa aaagataatt catccccccc agcgaaacgc cgtaaaacaa cagcggtaac
      721 cgctgctctt tggcaaagcg cagtcgccat ggcttaaaga acgcgatgag gatcagcgtt
      781 cctaatgcca gacgcagcgc agtgacaccc ggtgcgccca ccagaggaaa aagtgactta
      841 gctaacgagg ctccaccctg aatagacgcc atggcaacga gcaatatgac tattggtaac
      901 cagaccggca ttttacgtaa tgaaccaggc atcctttctc ccacaaatat ctaaacctaa
      961 gtaaagcgtg gagtgtactg gatataccca atgctggttg agcatttgtt gaaaaaattt
     1021 tcccccgttt tgactaaaat gcgccaggat tgatggaatc attagtctgg tgattaggaa
     1081 taatctggat gaatgacagg gaaaacatgc gtaatactta tgcagctctc tgaaaaagtg
     1141 atttaaattt agatggatag cggtgtatgg aaacgttctg ttacatgaaa tggcccgt
//
LOCUS       DAZFLD010000518         1190 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.521, whole genome shotgun sequence.
ACCESSION   DAZFLD010000518 DAZFLD010000000
VERSION     DAZFLD010000518.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1190
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.521"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>1190)
                     /gene="tssF"
                     /locus_tag="ACHKBL_005161"
     CDS             complement(<1..>1190)
                     /gene="tssF"
                     /locus_tag="ACHKBL_005161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009309580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HFO5821867.1"
                     /translation="LVSLLWPHYMRTIPSLAIVEFSPDWRSLRQAEILAEGFSVLSRP
                     VGPHKTACQYRTTRDVLLQPLHLADARLHTESDGRSAIRLRFECPEKVDWSKAGIDKV
                     AIFLNAEAPVSAALHLAMTRRVHAMYARHAGTYTGRHQFDGWCRPMGFDDNDCLWKKA
                     DTAFSGYQLLLEYFSFRPKFMFVELRGLDTIGLTAGSTWFEIDIVLSEAWSSGLPFET
                     ENFRLHCAPVINLFTLEADPLILNPLDNEYLLRPLRLQDGHTEIYSVDNIHGSVKNGR
                     HPYVPFTSFRHRGGMMRHDAPERYYHTRVKRGPSGLYDTWLVLGGRSFELEQLSDKPE
                     SLSMRITATNGQLPRRALESTLLDRVVKTGKVPVRVMNLTAPTMPLYPPANDRFHWRV
                     MSHLG"
BASE COUNT          274 a          326 c          351 g          239 t
ORIGIN      
        1 ccgaggtgac tcatcacccg ccagtgaaac cggtcatttg ccggaggata cagtggcatc
       61 gtcggtgcgg tgaggttcat gactctgacg gggactttgc cggttttgac caccctgtcc
      121 aggagcgtac tttccagcgc cctgcgcgga agctgcccgt tggtggcggt gattcgcatc
      181 gagagggact ccggtttatc agacagctgc tccagttcga acgagcgtcc cccgagaact
      241 aaccatgtat catacagacc ggaggggccc cgtttcacgc gggtgtggta ataacgttca
      301 ggagcatcgt ggcgcatcat accgccccga tgccggaagc tggtgaacgg gacataaggg
      361 tgtctgccat tcttcaccga gccgtgaatg ttatcgacgc tgtaaatttc ggtgtgacca
      421 tcctgaagac gcagcggcct gagcaggtac tcgttatcca gcgggttaag gatcagcggg
      481 tcggcttcca gggtaaagag attgataacc ggcgcacagt gcaggcggaa gttttctgtc
      541 tcaaaaggca aaccggatga ccaggcttca ctgagcacaa tgtcgatttc aaaccaggta
      601 ctgcccgccg tgagcccgat ggtgtcgagg ccgcgcagtt caacaaacat aaacttcgga
      661 cggaagctga aatactccag cagcaactgg taaccactga aggcagtatc ggctttcttc
      721 cacaggcagt cattgtcgtc aaagcccatg ggtctgcacc agccgtcaaa ctgatgacgt
      781 ccggtatatg tcccggcatg acgcgcatac atggcatgta cacgccgggt cattgccaga
      841 tgcagagcag cgcttaccgg cgcctctgcg ttaagaaaaa tggcgacttt gtcgatcccg
      901 gctttactcc agtccacttt ttcagggcat tcgaagcgca ggcggatagc agagcggcca
      961 tcggattcag tatggagacg ggcatctgca aggtgcagcg gttgtagcaa cacatcgcgg
     1021 gtcgtccggt actgacaggc ggttttatgc ggcccgacag gacgggacaa aacggagaag
     1081 ccttcagcca gtatttcggc ctgacgcaga ctacgccagt caggcgagaa ctcaacgatg
     1141 gccagcgacg gaatagtccg catatagtgc ggccacagca ggctcaccag
//
LOCUS       DAZFLD010000519         1190 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.522, whole genome shotgun sequence.
ACCESSION   DAZFLD010000519 DAZFLD010000000
VERSION     DAZFLD010000519.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1190
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.522"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>1190)
                     /gene="tssF"
                     /locus_tag="ACHKBL_005162"
     CDS             complement(<1..>1190)
                     /gene="tssF"
                     /locus_tag="ACHKBL_005162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009309580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HFO5821868.1"
                     /translation="LVSLLWPHYMRTIPSLAIVEFSPDWRSLRQAEILAEGFSVLSRP
                     VGPHKTACQYRTTRDVLLQPLHLADARLHTESDGRSAIRLRFECPEKVDWSKAGIDKV
                     AIFLNAEAPVSAALHLAMTRRVHAMYARHAGTYTGRHQFDGWCRPMGFDDNDCLWKKA
                     DTAFSGYQLLLEYFSFRPKFMFVELRGLDTIGLTAASTWFEIDIVLSEAWSSCLPFET
                     ENFRLHCAPVINLFTLEADPLLLNPLDNEYLLRPLRLQDGHTEIYSVDNIHGSVKNGR
                     HPYVPFTSFRHRGGMMRHDAPERYYHTRVKRGPSGLYDTWLVLGGRSFELEQLSDKPE
                     SLSMRITATNGQLPRRALESTLLDRVVKTGKVPVRVLNLTAPTMPLYPPANDRFHWRV
                     MSHLG"
BASE COUNT          273 a          325 c          353 g          239 t
ORIGIN      
        1 ccgaggtgac tcatcacccg ccagtgaaac cggtcatttg ccgggggata cagtggcatc
       61 gtcggtgcgg tcaggttcag gactctgacg gggactttgc cggttttgac caccctgtcc
      121 aggagcgtac tttccagcgc cctgcgcgga agctgcccgt tggtcgcggt gattcgcatc
      181 gagagggact ccggtttatc agacaactgc tccagttcga acgaacgtcc cccgaggact
      241 aaccatgtgt catacaggcc ggagggtccc cgtttcacgc gggtgtggta ataacgttca
      301 ggagcatcgt ggcgcatcat accgccccgg tgccggaagc tggtgaacgg gacataaggg
      361 tgtctgccat tcttcaccga gccgtgaatg ttatcgacgc tgtaaatttc ggtgtgacca
      421 tcctgaagac gcagcggcct gagcaggtac tcgttatcca gcgggttaag gagcagcggg
      481 tcggcttcca gggtaaagag attgataacc ggcgcacaat gcaggcggaa gttttctgtc
      541 tcaaaaggca aacaggatga ccaggcttca ctgagcacaa tgtcgatttc aaaccaggta
      601 ctggccgcag tgagcccgat ggtgtcgagg ccgcgcagtt caacaaacat aaacttcgga
      661 cggaagctga aatactccag cagcaactgg taaccactga aggctgtatc ggctttcttc
      721 cacaggcagt cattgtcgtc aaagcccatg ggtctgcacc agccgtcaaa ctgatgacgt
      781 ccggtatatg tcccggcatg acgcgcatac atggcatgta cacgccgggt cattgccaga
      841 tgcagagcag cgcttaccgg cgcctctgca ttaagaaaaa tggcgacttt gtcgatcccg
      901 gctttactcc agtccacttt ttcagggcat tcgaagcgca ggcggatggc agagcggcca
      961 tcggattcag tatggagacg ggcatctgca aggtgcagcg gttgtagcaa cacatcgcgg
     1021 gtcgtccggt actgacaggc ggttttatgc ggcccgacag gacgggacaa aacggagaag
     1081 ccttcagcca gtatttcggc ctgacgcaga ctacgccagt caggcgagaa ctcaacgatg
     1141 gccagcgacg ggatagtccg catatagtgc ggccacagca gactcaccag
//
LOCUS       DAZFLD010000520         1189 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.523, whole genome shotgun sequence.
ACCESSION   DAZFLD010000520 DAZFLD010000000
VERSION     DAZFLD010000520.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1189
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.523"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..1119)
                     /locus_tag="ACHKBL_005163"
     CDS             complement(<1..1119)
                     /locus_tag="ACHKBL_005163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HFO5821869.1"
                     /translation="MRKLSMVVCSVVSALMLCEATQAAEIYNKDGNKLDFYGRVKARH
                     YISDGSMDGDRTFARLGFKGETQINESLTGFGQWEYQFLGFKTESEVRNDKNRLAFAG
                     IRHNILGSLDYGRNYGVAYDVGALTDVLPDFGGDSWTYSDNYMTGRTTGVLTWRNTDF
                     FGLVDGLTFSAQYQGKNDRDNLLEANGDGYGFSVSYDYEGFGIVAAYTNADRTSLQEK
                     EGRKRFMTSKDSEGKEVITPRWIAGGKHAEFAGVGVKYNDNDLYLAADYSETRNMTPF
                     GSAGIADKARNIEVVAQYMFDFGLRPSLAYVQSKGMDVKGGIVNYGDQSLVEYIDVGA
                     NYFLNKNMSLLIDYKINLIDESEFTKKAGVATDNILAVG"
BASE COUNT          351 a          288 c          210 g          340 t
ORIGIN      
        1 accaacagca agtatattat cggttgcaac tccagccttt ttggtaaatt cgctttcatc
       61 aataaggtta attttgtaat caataagaag tgacatgttt ttgttcagaa agtagtttgc
      121 gccaacatca atatactcaa caagagactg atcgccatag tttactattc cacctttaac
      181 atccatccct ttggactgaa cgtaagccag tgaaggacgc aggccgaaat caaacatata
      241 ctgagccaca acttcaatat tccgtgcttt atctgcgatg cctgcgctac caaatggagt
      301 catatttcgg gtttcagagt agtcagctgc cagatacagg tcattatcat tatacttgac
      361 cccaacacct gcaaactccg catgttttcc cccggcaatc cagcgaggcg taataacttc
      421 ttttccttct gaatctttcg atgtcatgaa acgtttacgg ccttcttttt cctgcagact
      481 ggttctgtcg gcattagtgt aagcggcaac gattccaaaa ccttcatagt catagcttac
      541 ggagaaacca tatccatcac cattagcttc aagtaaatta tcacggtcat ttttaccctg
      601 atattgagca gaaaaagtaa gtccatcaac aagcccgaag aaatctgtat tgcgccaggt
      661 cagaactccg gttgttctgc cggtcatata gttatcagag taagtccagc tgtcaccacc
      721 aaagtccgga agtacgtcag tcagagcccc cacatcatag gcaacaccat agtttcgccc
      781 ataatcaagt gaaccgagta tattgtggcg aatacctgca aatgccagac gatttttatc
      841 gttgcgtact tcactttctg tcttaaaccc caggaactgg tattcccact gaccaaagcc
      901 agtcaggctc tcattaattt gtgtttcgcc tttgaaacca agacgtgcaa atgttctgtc
      961 gccatccata gaaccatcag agatgtaatg acgggcttta acgcgaccat aaaaatccag
     1021 tttattaccg tctttattat agatttctgc tgcctgtgtt gcctcacaca gcattaatgc
     1081 agaaacgaca gagcacacaa ccatacttaa ttttcgcata aaaaaatccc ctgtgcacat
     1141 ttttagtatg gttttaaaaa ttaaactatg gcgaaaaaat tatcactat
//
LOCUS       DAZFLD010000521         1178 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.524, whole genome shotgun sequence.
ACCESSION   DAZFLD010000521 DAZFLD010000000
VERSION     DAZFLD010000521.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1178
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.524"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            223..411
                     /locus_tag="ACHKBL_005164"
     CDS             223..411
                     /locus_tag="ACHKBL_005164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1187 family protein"
                     /protein_id="HFO5821870.1"
                     /translation="MGKRYKITATIIKPGNMPVQWLRYQDSEMTFAECEKLFAPAKEP
                     GRYIRVRVEDFRCEVVKA"
     gene            complement(414..603)
                     /gene="tnpB"
                     /locus_tag="ACHKBL_005165"
                     /pseudo
     CDS             complement(414..603)
                     /gene="tnpB"
                     /locus_tag="ACHKBL_005165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309368.2"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase.; frameshifted; incomplete; partial
                     in the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            complement(590..798)
                     /locus_tag="ACHKBL_005166"
                     /pseudo
     CDS             complement(590..798)
                     /locus_tag="ACHKBL_005166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309369.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
BASE COUNT          315 a          253 c          311 g          299 t
ORIGIN      
        1 agtctccact ggcgttgaca atgtggcaga aggagggtgt caagaactct gaaaaataac
       61 gagagagaca tgttactctg atatctttgt tgtattctga ggtgccatcg gtgcccgtac
      121 agaatcatcg catgagccat tgctgagacg ctcacttttt ttcgcatgct ttaccggagt
      181 gatggctcag ccggtaattt tgttgtcagg atgagaggcg atatggggaa acgatataaa
      241 ataactgcga caataatcaa acccggtaat atgccggttc agtggttgcg ttaccaggat
      301 tcagaaatga cattcgccga atgtgaaaaa ctgtttgcac ctgcgaaaga gcccggacgg
      361 tatattcgtg tcagggtgga agacttccgg tgtgaagtag tgaaggcctg acgcagccat
      421 acgccatgtt tatcccggcg tagaactttt atgcgggaac gagccttgtt ggtaaagaca
      481 aaggcagcat tgtcgtgcca tgcagcgtta agttctttcc ggacgtactg tgtcagtttt
      541 gtcgatgccc cggcgcatat tgacgggttt tacgaccagg aatacgttat cagtcctcag
      601 tatggcaaaa agcccggaaa acatcggtta actgagaagg ctggcagcac atccggatac
      661 caccgggaag gaagaggata aggctcatcc gacaatgatc tgttatcggc cactccatgg
      721 gcaacagata ctgaggcgag gaatgattgc aggcgctctg gtccgttttt cggcttttgc
      781 ggcatcctgt ggtcgctgat gcgccatgaa gcaatatgat gcttttctgg tcaagcgctc
      841 atcgcattcc agggattgtt tattctttct ccaccgaagg tggttatacg aataccggaa
      901 tgctaatgaa cgaaatattt ctgtgaagat ggtagaacag agatgtgacg ccatcgctgt
      961 catacgggaa tgggtatata tcaggatttt tataaccgtc agcgccctcc ctcacggcag
     1021 gggaatacca ctccggtagc attcaggaaa agtatcatcg gatgaatgct taaaaaaaga
     1081 acaaatgact gtatccgcta ttgccagtac acctcagact tcgatgccag tccgtgaacg
     1141 aacagagtgg aatacgtagt catactacat atggtgtt
//
LOCUS       DAZFLD010000522         1174 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.525, whole genome shotgun sequence.
ACCESSION   DAZFLD010000522 DAZFLD010000000
VERSION     DAZFLD010000522.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1174)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1174)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1174
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.525"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..589
                     /locus_tag="ACHKBL_005167"
     CDS             <1..589
                     /locus_tag="ACHKBL_005167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF6645 domain-containing protein"
                     /protein_id="HFO5821871.1"
                     /translation="VDKNIAEAVVSDEGATGGKALQLNKPESHIWFLEHDAAGKGAEL
                     LKKGGRVSVRFKVPGALVPNQFALGIYWQLSSLPEGVTLSEEGNDMLMSFFLQTDATN
                     LNAMYHKKQNAKLDTFGVFDNGWHTLAFEFAGNNSIQVTPVLDEKRGTPFTLVKSPAS
                     GAADKLQLTGISKAATYTLLIDSVKVEVNNADAAA"
     gene            740..919
                     /locus_tag="ACHKBL_005168"
     CDS             740..919
                     /locus_tag="ACHKBL_005168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1378 family protein"
                     /protein_id="HFO5821872.1"
                     /translation="MTFLNQLMLYFCTAVCVLYLLSGGYRAVRDFWRRQIDKRAAEKI
                     SASQSAGSKPEEPLI"
     gene            960..>1174
                     /locus_tag="ACHKBL_005169"
     CDS             960..>1174
                     /locus_tag="ACHKBL_005169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF826 domain-containing protein"
                     /protein_id="HFO5821873.1"
                     /translation="MSEITSLVTAEAVKEVLRSEEVLSALKQKLRQNLEARLDAEVDA
                     ILDELLGAPAVPEPEGIAGEGSASDGG"
BASE COUNT          306 a          268 c          360 g          240 t
ORIGIN      
        1 tgttgataaa aacatcgctg aggccgtggt cagtgatgag ggtgccaccg gtggtaaggc
       61 cctgcaactg aacaaaccgg aaagccacat ctggtttctg gagcatgatg ccgccgggaa
      121 gggggcagag ttgctgaaga aaggcggacg tgtgagcgtg cggtttaagg tgccgggcgc
      181 actggtgccg aatcagtttg ccctgggtat ttactggcag ttgtcgtccc tgccggaggg
      241 agtgacgctg tcagaggaag gcaacgacat gctgatgtcc ttcttcctgc agacggatgc
      301 gacgaacctg aacgcgatgt accacaagaa gcagaatgcg aagctggata cgttcggggt
      361 ctttgataac ggatggcaca cactggcttt tgagtttgcc ggaaacaaca gcattcaggt
      421 gacgccggta ctggatgaga aacggggtac accgttcaca ctggtgaaat ctccggcatc
      481 aggggcggcg gacaaactgc aactgacagg catatcaaag gcggcgacat atacgctgct
      541 gattgacagt gtgaaggtgg aagtgaacaa cgcggacgcc gcggcatgat aaaaaaggcc
      601 gccagcaatg aaggaaaggg atgaactgtg tccactacat gaatgctggc ggctggtaaa
      661 acgaccatga tgacaccgaa gtctgcggtg tttttctaca aaaataagta aatcatgtca
      721 actatcacgg tgagaaacca tgacatttct gaaccagtta atgctgtact tctgtacggc
      781 ggtctgtgtg ctgtatcttc tttcgggtgg gtacagggca gtgcgcgatt tctggcgcag
      841 gcagattgat aaaagggccg cagagaaaat cagcgccagt cagtcagccg gaagcaaacc
      901 cgaagagccg ctcatttagc tgcaactttc ttaatcacac ctttcgacga gaaaatccca
      961 tgtcagaaat tacatccctg gtcactgcag aggcagtgaa ggaagtcctg cgctctgaag
     1021 aagtcctgag cgcactgaaa cagaaactcc gccagaacct tgaggcgcgt cttgatgcag
     1081 aagtggatgc cattctggat gaactgctgg gcgcaccggc agttccggag ccggaaggta
     1141 tcgcgggtga ggggagtgct tcagatggcg gtga
//
LOCUS       DAZFLD010000523         1172 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.526, whole genome shotgun sequence.
ACCESSION   DAZFLD010000523 DAZFLD010000000
VERSION     DAZFLD010000523.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1172)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1172)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1172
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.526"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..654
                     /locus_tag="ACHKBL_005170"
     CDS             <1..654
                     /locus_tag="ACHKBL_005170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000200205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VasL domain-containing protein"
                     /protein_id="HFO5821874.1"
                     /translation="IPGWQFLTQPSPAEQQLRALLTPPPDVLSADQIAQLSRTRSLLN
                     HTSEWIPLSGQQITRLTELPLAYNLQRSAQLLQQLRVLFPDNPRVQEMVDNWQKSVRS
                     RALPEEAMTGWNEGMTRLQQLAERLNRLDEQRGKYMTVSELKTEVFGIMQAFNRHIPA
                     EEQLRRYNEVRNQNGSEQQQKLTQDALTELLNRYWFLRHGDAGKKTINNKQDNQINI"
     gene            797..886
                     /locus_tag="ACHKBL_005171"
                     /pseudo
     CDS             797..886
                     /locus_tag="ACHKBL_005171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          345 a          257 c          319 g          251 t
ORIGIN      
        1 attccgggat ggcagtttct gacacagcca tcaccggcgg agcagcaact cagggcgctg
       61 ctgacacccc caccggatgt gctttcggca gaccagatag cacagctcag cagaacacgc
      121 tcactgttaa atcatacgtc agagtggata cccctttccg gtcaacagat aacgcgactg
      181 acagaattac ccctggcata caacctgcag cgcagtgcgc agctgctgca gcaactgagg
      241 gtacttttcc ccgataatcc ccgggtacag gaaatggtgg ataactggca gaagagtgta
      301 aggagccgtg cgctcccgga agaggcgatg acgggctgga acgaaggcat gacccgccta
      361 cagcagctgg ctgagcgctt gaaccgtctg gatgaacagc ggggaaaata catgacagtc
      421 agtgaactga aaacagaggt gtttggcatc atgcaggctt ttaaccggca tatcccggca
      481 gaagagcagt tacgtcgcta taatgaagtc cgcaaccaga atggcagtga acaacagcaa
      541 aaactgacgc aggatgccct gacggaactg ttgaaccgct actggtttct gagacatgga
      601 gatgcaggta agaagacgat aaataataaa caggataacc agattaatat ctgacagatt
      661 ttattgccat tatttatcca gaagcgatga tgccattaat gcaaaatgac atctgaattg
      721 atcacagatt ggaccaatct gtgatcaaat agaacagaat acgcagatga taaagcggct
      781 ttccctcgaa gattcacaac gtcatctggc agaatatggg tgagagttgc aggctgatgc
      841 gtacaaagct tacgatgcgt tgtatgaaac tggccgggtg aaagatgtca tccgtacggt
      901 gcgcgccaga ctaaacaggc atcatgatgg aaaagtcggg tatcattcac aggcatattt
      961 gtcagaatgg tagcgtcagc gggttttgat tacaccagtt ttgaagctca tacttcctat
     1021 gaggcactgc agaaaacgaa agtgtcgttt aggcgctggg ggaggggtta aaaaagtaac
     1081 ggcagaagtc accctgaagt tgaggcagtc aactactgtt agctggctgc tgcatgagag
     1141 tgtggaggga gattgtatat attggttagt ca
//
LOCUS       DAZFLD010000524         1168 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.527, whole genome shotgun sequence.
ACCESSION   DAZFLD010000524 DAZFLD010000000
VERSION     DAZFLD010000524.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1168)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1168)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1168
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.527"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            61..>1168
                     /locus_tag="ACHKBL_005172"
     CDS             61..>1168
                     /locus_tag="ACHKBL_005172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000702796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase-like activity of phytase family
                     protein"
                     /protein_id="HFO5821875.1"
                     /translation="MKIRYAAIFLALPLLCTTAQANSFLHYAGEFNFRTGEKQNNVTI
                     AGLSAITFDAKKNIFYAINDSRNNNKEGDASLYTLKIQVSSRGIEQVNFLNQRPLLDA
                     KQQPFVTNTVDAEGLALTHNGKSLLWSSELGAPLRLSTLDGVMEKDFTSLFPARFNIS
                     SDKESSNGIRSGNAWEGLTVTPDGKSLFIAVESSLKQDGPIASPINSGTSRLLQFSID
                     ADGRPSKQLHEYLYITDPVPQVSKFGINDNGVSEVMALNDHQLLVIERSGRNVSAGFN
                     DWDYSVRVYMVDLTAASDIKNIDSLQDWSNKSTLQPVSKKLLIDFADYTSSADCIEGV
                     TFGPLIDGHTSLIFVSDNNFQPHQQTKFYLFIDKE"
BASE COUNT          354 a          238 c          244 g          332 t
ORIGIN      
        1 gaggttaatc tttttctttc acaacacgac taaaatcata tatatacaaa ggtaaatatc
       61 atgaaaataa gatatgccgc tatttttctg gcattaccgc tgttatgcac aacagcgcag
      121 gcaaacagtt ttcttcatta tgctggggag tttaacttcc ggacgggaga aaagcagaac
      181 aatgtcacaa tcgcggggct atcagccatc acatttgatg caaaaaaaaa tattttctat
      241 gctattaacg atagcagaaa caataataaa gaaggagatg ccagtcttta tacactaaaa
      301 atacaggtat catccagggg cattgagcaa gtgaattttc tcaaccagcg tccactgctt
      361 gatgcaaaac agcaaccttt cgtgaccaat acggtggatg ccgaaggtct ggccctgaca
      421 cacaatggta aatcattact ctggagttcg gagttaggtg ctccactgcg tctgagcact
      481 ctcgatggcg taatggagaa agattttacg tctttatttc ctgctcgctt taatattagt
      541 tctgacaaag agagcagcaa tggtattcgc agcggcaacg cctgggaggg cctgacagtt
      601 acaccagatg gaaaatcact ttttatcgcc gttgaaagca gccttaaaca ggatgggcct
      661 attgcaagtc caataaattc aggcacatca cgcttactgc aattttctat agatgcagac
      721 ggtcgacctt caaagcaatt gcatgaatat ctctatatta ctgatcctgt tccgcaagtg
      781 agtaagttcg gtattaatga taatggcgta tccgaggtca tggcgctgaa tgatcaccaa
      841 ttgttggtca tcgaacgttc cggcagaaac gtcagtgctg ggttcaatga ctgggattac
      901 tctgtcagag tttatatggt cgatttaact gctgccagcg atatcaagaa tattgacagt
      961 ctgcaagatt ggtccaataa atccacgtta caacccgtca gtaaaaaatt attaattgat
     1021 tttgctgatt acacttcctc tgcggattgt attgaagggg taaccttcgg tcctctgatt
     1081 gatgggcata catcgttaat atttgtatct gataataatt tccagcccca tcaacagacg
     1141 aaattttatc tattcattga taaggaaa
//
LOCUS       DAZFLD010000525         1166 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.528, whole genome shotgun sequence.
ACCESSION   DAZFLD010000525 DAZFLD010000000
VERSION     DAZFLD010000525.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1166)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1166)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1166
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.528"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>1166
                     /locus_tag="ACHKBL_005173"
     CDS             <1..>1166
                     /locus_tag="ACHKBL_005173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001279669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication endonuclease"
                     /protein_id="HFO5821876.1"
                     /translation="LNPKYCGATVRDASDYLVYRFFAAVRRAINKAGLGWYGVRTVEP
                     HHDGTPHWHMLVFTSPENEARITEIMRNAAIREDRAELGDDISPRFKCEKIDPAKGTP
                     TSYIATYIGKNLDASAFMGNDPKTGKPYVDKESGKTMAETVENAIGWAGLHRIHQFQF
                     FGIPPRQVWRELRRLAGQMARNPAAPQRLDHDDIDAILAAADVGCFATYITRQGGVLI
                     PRNTYLVRTAYETTEEANDYGEFPQRIYGVRAPSLGERYTICTHPDTWKLVRRKPENE
                     DRTDEGFDVECGFAAPWTRGNNCPPDQNLTINDPPERQPDGRREQRLTLPEGDLGEWL
                     RSLTPTERKQLTRQLKNPPPDTETVKNGGISAPAGGVRVSVALTAEPHITAQITR"
BASE COUNT          287 a          379 c          314 g          186 t
ORIGIN      
        1 ctgaacccga aatactgcgg agcaacggta cgtgatgcca gtgattatct ggtttaccgc
       61 tttttcgccg ccgtccgcag ggccatcaac aaggccgggc tgggctggta cggcgtgcgc
      121 acagtagagc cccaccacga cggtacaccg cactggcaca tgctggtatt caccagcccg
      181 gaaaacgaag cccgtatcac tgaaatcatg cgcaacgccg ccatcaggga ggaccgcgca
      241 gagctgggtg atgatatctc cccgcgtttt aaatgcgaaa aaatcgaccc ggcaaaaggc
      301 acaccgacaa gctatatcgc gacctatatc ggtaagaacc tggacgccag cgccttcatg
      361 gggaatgacc cgaaaaccgg caaaccttac gtcgataagg aatcaggcaa aaccatggcc
      421 gaaaccgtgg agaacgccat cggctgggca ggccttcacc gcatccacca gttccagttc
      481 ttcggtatcc ctccccgcca ggtatggcgc gaactccgcc gtcttgccgg gcaaatggcc
      541 cgtaacccgg ctgccccgca gcgcctggat catgacgaca ttgacgccat actggccgcc
      601 gccgacgtgg gatgttttgc cacctatatc acccgccagg gtggtgtgct cattccccgc
      661 aacacgtacc tggtccgcac cgcatacgaa actaccgaag aggccaacga ctacggcgaa
      721 ttcccgcaac gcatttacgg cgtccgcgcc ccgtcactcg gggagcgcta caccatatgc
      781 acccacccgg acacctggaa actggtcaga cgtaagccgg aaaacgaaga ccgcacggac
      841 gagggttttg acgttgagtg cggctttgcc gcaccttgga ctcgtggcaa taactgtccc
      901 cctgaccaga atctgaccat aaacgacccg ccagaaaggc agccggacgg acgcagggag
      961 cagcgactta ccctgccgga aggtgatttg ggtgaatggt taaggtcact gacccccaca
     1021 gagcgaaaac aactcaccag gcagctgaaa aacccgccac ctgacacgga aaccgtgaaa
     1081 aacggcggca tcagtgcgcc cgctggcggc gttcgcgtaa gtgtcgcact gaccgctgag
     1141 ccacacatca ccgcacaaat cacccg
//
LOCUS       DAZFLD010000526         1144 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.529, whole genome shotgun sequence.
ACCESSION   DAZFLD010000526 DAZFLD010000000
VERSION     DAZFLD010000526.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1144)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1144)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1144
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.529"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..773
                     /gene="yeeO"
                     /locus_tag="ACHKBL_005174"
     CDS             <1..773
                     /gene="yeeO"
                     /locus_tag="ACHKBL_005174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001323172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
                     /protein_id="HFO5821877.1"
                     /translation="AILWVLAIGFNPALRISLKSYFKPLNFSIIWEVMGIGIPASVES
                     VLFTSGRLLTQMFVAGMGTSVIAGNFIAFSIAALINLPGSALGSASTIITGRRLGVGQ
                     IAQAEIQLRHVFWLSTLGLTAIAWLTAPFAGVMASFYTQDPQVKHVVVILIWLNALFM
                     PIWSASWVLPAGFKGARDARYAMWVSMLSMWGCRVVVGYVLGIMLGWGVVGVWMGMFA
                     DWAVRAVLFYWRMVTGRWLWKYPRPEPQKCEKKPVVSE"
     gene            874..949
                     /locus_tag="ACHKBL_005175"
     tRNA            874..949
                     /locus_tag="ACHKBL_005175"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:907..909,aa:Asn,seq:gtt)
BASE COUNT          245 a          241 c          307 g          351 t
ORIGIN      
        1 ttgcaatttt gtgggtgctg gcgattggtt ttaatcctgc gctaaggatt tcgttaaaga
       61 gctattttaa accgctgaat tttagcatta tctgggaagt catggggatt ggtattccag
      121 cgagtgtcga atcagtgtta tttaccagtg gtcggttatt aactcaaatg ttcgttgccg
      181 ggatggggac cagcgttatt gccggaaatt ttatcgcgtt ctcaattgcg gctcttatca
      241 acttacccgg aagtgcgctc ggttctgcct ctacgatcat tacaggccga aggttgggag
      301 tagggcagat agcgcaagca gagattcagt tgcggcatgt gttctggctg tccactcttg
      361 gattaacggc catcgcctgg ctaacggctc cctttgccgg ggttatggca tcgttttaca
      421 cccaggatcc acaggttaaa cacgtcgttg tgattctgat ttggctaaat gctttattta
      481 tgcctatttg gtccgcctca tgggtgctac ccgctggatt taagggggct cgcgatgcac
      541 gttacgccat gtgggtttct atgttgagca tgtggggttg ccgggttgta gtcggttatg
      601 tgctgggaat catgcttggc tggggtgtgg ttggtgtctg gatgggaatg tttgccgact
      661 gggctgtgcg ggccgtgctg ttttactggc gaatggttac tggacgttgg ctgtggaaat
      721 accctcgacc cgagccgcaa aagtgtgaaa aaaagccagt tgtgtcggaa taaacgacaa
      781 aatgcagatt atttcagcaa acgatttcaa atttaaaaaa caggctttga cattgtggat
      841 gggcatcgct aatattcgcc tcgttctcac gattcctctg tagttcagtc ggtagaacgg
      901 cggactgtta atccgtatgt cactggttcg agtccagtca gaggagccaa attctaaaaa
      961 ttcgcttttt agcgcaatgt cactgacctt agttgagcat tgctttttaa cggatagcgg
     1021 gtttttgaca tcttaagcgc cctcgacctt tatggttgag ggcgttttgc tatgaacgcc
     1081 atcaccattt tcccctcgat tacaaaactt gagtaattca gtagtctccc ctcttgcaac
     1141 tcac
//
LOCUS       DAZFLD010000527         1137 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.530, whole genome shotgun sequence.
ACCESSION   DAZFLD010000527 DAZFLD010000000
VERSION     DAZFLD010000527.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1137
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.530"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(158..616)
                     /locus_tag="ACHKBL_005176"
     CDS             complement(158..616)
                     /locus_tag="ACHKBL_005176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001426131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5821878.1"
                     /translation="MHSLLILLYVVVWRACTILRVIAPVSAVRAWCEYQLEGMTILRD
                     AYSSLAKAAGMQNDAFEYTESIARHLGYKSGGDQLKSGLFRPFLWHIRVKGHRLGAAM
                     EMEAWKTWKNGCNDVCNPWERGWNDGWTECLNQRIAGLLKQACIEFSDAD"
     gene            complement(699..1091)
                     /locus_tag="ACHKBL_005177"
     CDS             complement(699..1091)
                     /locus_tag="ACHKBL_005177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5821879.1"
                     /translation="MSTFILCPEPGISSFFRRMTTQLRVFFSRNNLSYYSRWYTNPAG
                     NLSVRVCMFRRGGVWTPCLYYRQGMYRNEYTGWYQPLPREVIYLNRFTGRISRRRTWH
                     AACRMAEQLTTLRYRFGLQGDGIVAQPC"
BASE COUNT          321 a          323 c          261 g          232 t
ORIGIN      
        1 ggaacaaaac ttccgtcagg gaaaacaccg tcgcaaaaac acggtgcagt aagtaaaaga
       61 aaaaccgtta cgggagcaaa acagccatca ttgcaaaatg gcagttgcat cgctcacgat
      121 ttgacatcct tcctgtgact gactggcatc ctgcccgtca gtctgcgtcg gaaaattcaa
      181 tacaggcctg cttcaggagc ccggctatac gttgattcag acattccgtc cagccatcgt
      241 tccatccacg ctcccatgga ttgcagacat cgttacagcc attcttccat gtcttccatg
      301 cctccatctc catggcagcc cccagcctgt ggcctttcac acgtatatgc cacagaaacg
      361 gccggaaaag tcctgatttc agttggtcac caccgctttt gtagcccaga tgacgtgcaa
      421 tactttcagt atattcaaat gcatcattct gcattccagc cgccttagcc agcgagctgt
      481 acgcatcacg aagtatcgtc ataccttcca gttgatactc acaccacgca cggacagccg
      541 acactggtgc gattacccga agaatggtgc aggcacgcca tacaacaacg taaaggagta
      601 ttaacagact atgcatgtgt tcacctcaga tacaaaaaat aacgggaaca cacctgccct
      661 gcgggaagcg tgttcccgac agggtaataa atgataaatc agcagggctg tgcgacgatt
      721 ccatcaccct gcagaccaaa ccggtaacgc agggtggtca gttgctccgc catacggcag
      781 gcagcatgcc atgtcctgcg acgggatatc cgtccggtaa accggttcag ataaatgacc
      841 tcacgcggta acggctgata ccatcctgtg tactcgttgc gatacattcc ctgccggtaa
      901 tacaggcagg gtgtccagac accgccacga cggaacatac agacccgcac gctcagattg
      961 ccggccggat tagtgtacca gcgactgtaa tatgacaggt tattgcggga aaaaaacacc
     1021 cgcaactgag tcgtcattcg ccggaaaaaa gaagagatgc ccggttcagg gcacaaaata
     1081 aacgttgaca taaagactcc tgtaaaaaaa cgggagcctt cgccccgacg gacaaaa
//
LOCUS       DAZFLD010000528         1126 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.531, whole genome shotgun sequence.
ACCESSION   DAZFLD010000528 DAZFLD010000000
VERSION     DAZFLD010000528.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1126
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.531"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            117..374
                     /locus_tag="ACHKBL_005178"
     CDS             117..374
                     /locus_tag="ACHKBL_005178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020884766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAAR domain-containing protein"
                     /protein_id="HFO5821880.1"
                     /translation="MLHIIRKGDKTTHGGSVLTASETMKFGGIGVARKGDKVSCPGHG
                     PTIIIEGNPDYLDHGIPVAFHGHKCACGCTLISSFAAAKVA"
     gene            377..>1126
                     /locus_tag="ACHKBL_005179"
     CDS             377..>1126
                     /locus_tag="ACHKBL_005179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004170999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5821881.1"
                     /translation="MPVWLDAIPEKAPKIARPNTRRWLLFLAFIMLGGMALTLWCWTS
                     ERTGFVFWFTALGLPFCTWGLIFGLRRFVYKAGQVGAESRNVEREALIDSEILRGQRC
                     AWILGTYIQSPAGNKADDLLEAMEVAAPAIDFSHPRGCDKPVRYAALPEYQTDLTKAL
                     KGAVNKLTTRVEGIVHPLPPALPCWLVLDCDNDLYPLIEEQLKADLSLKTGRIFRLMT
                     GKGLGAFDAWLDKRWDTPGILVVITLSLPASP"
BASE COUNT          271 a          266 c          310 g          279 t
ORIGIN      
        1 cccggaaaca ttcataataa aaatccagac aatcactatc gatggcagag cccagcataa
       61 acatgggaat taaagagtga tgaattagtt aagagataag aaagaggaat ttaataatgc
      121 ttcatattat tcgtaaaggc gataaaacta ctcacggagg ttccgttctg accgcttctg
      181 aaacgatgaa attcggtggg ataggcgtcg cccgtaaagg cgataaagtg tcatgtccgg
      241 gtcatggtcc taccatcatc atagaaggca atcctgatta tcttgaccat ggtatcccgg
      301 tggcatttca cggtcataaa tgcgcctgtg gttgcacatt gatcagttca tttgccgcag
      361 caaaggttgc ctgattatgc ctgtctggct ggatgccatc cctgaaaagg ccccaaagat
      421 agcgcgcccc aatactcgcc gctggctgtt gttcctggca tttatcatgc tgggggggat
      481 ggcgctcaca ttatggtgct ggacgtcaga acgaacagga tttgtcttct ggttcaccgc
      541 tctggggctt ccattttgca cgtgggggct tattttcggt ttacgtcggt ttgtctataa
      601 agccgggcag gtgggggctg agtcccgaaa tgtcgagcgg gaagcgctta ttgacagtga
      661 aattctgcgt ggtcagcgct gtgcctggat cctcggtact tatattcaga gcccggccgg
      721 aaacaaagcc gatgacctgc ttgaggcaat ggaagtcgcg gctcctgcta ttgatttctc
      781 gcacccaaga ggatgtgata aaccggtcag gtacgcggca ttaccagaat accagacaga
      841 tttaacgaag gctctgaaag gggcggtgaa taaactgacc acgcgtgtag aggggatagt
      901 tcatccgctt ccgcccgcgc taccatgctg gctggtgctg gattgtgata acgatctgta
      961 tcccctgatt gaagagcagc ttaaagccga cctctcactc aagacgggca ggatttttcg
     1021 cctgatgacc ggaaaagggc tgggggcgtt tgatgcctgg ctggacaagc gatgggacac
     1081 cccgggcatt ctggttgtca tcacgctgtc gttacctgcc tctccc
//
LOCUS       DAZFLD010000529         1125 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.532, whole genome shotgun sequence.
ACCESSION   DAZFLD010000529 DAZFLD010000000
VERSION     DAZFLD010000529.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1125)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1125)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1125
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.532"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            5..1125
                     /locus_tag="ACHKBL_005180"
                     /pseudo
     CDS             5..1125
                     /locus_tag="ACHKBL_005180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700772.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
BASE COUNT          273 a          265 c          354 g          233 t
ORIGIN      
        1 gtttatgaaa ataaatcgac acggtattac tttatcgctt atttcctttc tgattgccgg
       61 tgctccggcg tttgcgcagg atggcagttc ctgggcgtct ccggacacag cctctgcatc
      121 agagactgac tccacggcag tgggggcatg ggcgaatgcc gggaaggcag ggacagctct
      181 tggctctgca tcgtatgcgc atgcgccgtt ttccacatcc gtggggatgt ctgcttatgt
      241 aaatccggag gcgtctaatg gtgtggcagt aggtagctgg gcatctgcag atgctttaaa
      301 ttcagtagca ctgggtacgg gtgcacaggc aatgcacagg tattcagtgg ctcttggtca
      361 gcagtccggt acaacccgtg aaaatgaagt ctacatcgga tatgactcag gtgtcacaaa
      421 cccggccagt ccgcgtgtac cggataaaac ccgggttctc ggtggtgtca gtgatggtac
      481 acgggatacg gatgcagcca ctgtcgggca actgaaccgg aaggcggatg aggtgtacag
      541 tgacgtcagt ggacggattg cagcggaagc acttaaggcg cgtgaccata ccgatacggt
      601 tgctgcagag aacagggaga atattatccg gaacacggtg gccataaacc gtaacaccag
      661 gggctgcttt cccagagaga cgtgctggag acgcatgaag agcgcctgaa cagccagcaa
      721 cagcagataa ataccggaag taccgttgct gtggacagtc atggttatgt gacgcgcggt
      781 gagggaaccg gtgaacgaat tactgtacag gaagggctgg tacgtacaca ggaaatggcc
      841 acagaaaacc gtgctgcggt aagccgtaac cgtcaggtgg gtgaacgcaa cagtcgcgcc
      901 attgcttctc aggcacgtac cctgcaggcg catgagcgtc gtatgaacag tcagcagcgt
      961 cagattaatg agaatcatga ggaaatgaag cgggcagcag cccagtcagc agcgctggcc
     1021 ggtcttttcc agccatacag tgtgggtaaa tttaacgcca cagcggctgt gggcggacag
     1081 tgacaggcag gctgttgcgg tgggcatggg atatcgcttt aacga
//
LOCUS       DAZFLD010000530         1123 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.533, whole genome shotgun sequence.
ACCESSION   DAZFLD010000530 DAZFLD010000000
VERSION     DAZFLD010000530.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1123)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1123)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1123
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.533"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            139..1077
                     /gene="tdcA"
                     /locus_tag="ACHKBL_005181"
     CDS             139..1077
                     /gene="tdcA"
                     /locus_tag="ACHKBL_005181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TdcA"
                     /protein_id="HFO5821882.1"
                     /translation="MSTILLPKTQHLVVFQEVIRSGSIGSAAKELGLTQPAVSKIIND
                     IEDYFGVELVVRKNTGVTLTPAGQLLLSRSESITREMKNMVNEISGMSSEAVVEVSFG
                     FPSLIGFTFMSGMINKFKEVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSAEMKL
                     QDLHVEPLFESEFVLVASKSRTCTGTTTLESLKNEQWVLPQTNMGYYSELLTTLQRNG
                     ISIENIVKTDSVVTIYNLVLNADFLTVIPCDMTSPFGSNQFITIPVEETLPVAQYAAV
                     WSKNYRIKKAASVLVELAKEYSSYNGCRRRQLIEVD"
BASE COUNT          319 a          203 c          249 g          352 t
ORIGIN      
        1 tgatatataa agatatttaa ttgtaataac gataccctgg aaagtattga aagttaattt
       61 gtgagtggtc gcacatatcc tgttcatttc attttgatac acttcatgcc gtcaatgagg
      121 taattaacgt aggtcgttat gagcactatt cttcttccga aaacgcagca cctggtagtc
      181 tttcaggaag ttattagaag tggttctatc ggctcggctg caaaagagtt agggttaact
      241 caaccggccg tcagtaaaat cattaacgat attgaagatt attttggtgt ggagttagtg
      301 gtgcggaaaa ataccggtgt aacattaaca cctgccggtc aattgttact ctcccgttcc
      361 gaatccatta cccgtgaaat gaaaaatatg gttaatgaga taagcggtat gtcttctgag
      421 gcggtggtgg aagtctcatt tggttttcct tcattgattg gttttacttt tatgtcaggg
      481 atgatcaaca agttcaaaga ggtgttcccg aaagcgcagg tttctatgta tgaagcgcaa
      541 ctgtcttcgt tcttaccggc aatccgcgac ggtcgactgg attttgcgat tggtacgtta
      601 agcgcagaaa tgaagcttca ggatttacat gttgagccgc tgttcgagtc cgagtttgtg
      661 ctggtagcca gtaagtcccg aacatgcacc ggcaccacca cgctggagtc gttgaagaac
      721 gaacagtggg tgttgccaca aaccaatatg gggtactaca gcgaactact tactacgtta
      781 caaagaaatg gcatcagtat tgaaaacatc gttaaaaccg actcagtcgt gacaatttat
      841 aatcttgttc tcaatgctga tttcttaact gtaattcctt gtgatatgac atcacctttt
      901 ggttctaatc aatttattac tattccggtt gaagaaacat tacctgtggc acaatatgcc
      961 gcggtatggt cgaaaaatta tcgtattaaa aaagcagcat cggttttggt ggaattagcc
     1021 aaagagtatt catcttataa tgggtgtaga cgaaggcaat taattgaagt tgattagtta
     1081 tttgttttaa tttaaacata aataatccac ctgtctgttt tgc
//
LOCUS       DAZFLD010000531         1122 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.534, whole genome shotgun sequence.
ACCESSION   DAZFLD010000531 DAZFLD010000000
VERSION     DAZFLD010000531.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1122)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1122)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1122
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.534"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..408)
                     /locus_tag="ACHKBL_005182"
     CDS             complement(<1..408)
                     /locus_tag="ACHKBL_005182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc ribbon protein"
                     /protein_id="HFO5821883.1"
                     /translation="MNGIKTGLGISPGEHIISADSALSRNIRQCFCLSCRGRLILQTD
                     AQGAWFEHDSHALSAQQKATCVVLNPEKSHPYIEDMAMFLSPLPVVLEWHCVMCEQFF
                     HGKKFCEACGTGIYCRATCTKSVYRYQPDLCQDC"
     gene            complement(405..788)
                     /locus_tag="ACHKBL_005183"
     CDS             complement(405..788)
                     /locus_tag="ACHKBL_005183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc ribbon protein"
                     /protein_id="HFO5821884.1"
                     /translation="MYAKSFIAFDGNGRLTGARTAQTAPYDRYTCHLCGSALRYHPQY
                     DTERPWFEHADEGLTEHAQQCPYVRPERREIRLIKRLQQFVPDALPVVRKASWRCRQC
                     HHDYYGERYCTHCHTGHFSEEGVAE"
BASE COUNT          267 a          304 c          295 g          256 t
ORIGIN      
        1 gcagtcctgg cacaaatctg gctggtacct gtaaacagac ttcgtacatg ttgctctgca
       61 ataaatcccc gtaccacagg cctcacagaa tttcttccca tgaaaaaact gctcacacat
      121 cacacaatgc cactccagca cgaccggtaa tggcgataga aacatcgcca tatcctcaat
      181 gtaagggtgg gacttttccg gattcagcac cacgcaggtc gccttctgtt gcgcgctcag
      241 ggcatgtgaa tcatgctcaa accacgcccc ctgagcatct gtctgcaaaa tcaaccgacc
      301 acgacaggaa aggcagaaac actgccggat atttctgctg agcgcagagt cggcactgat
      361 aatgtgttca cccggcgaga tccccagccc cgtttttata ccgttcattc agccactccc
      421 tcctcactga agtgccctgt atggcagtgt gtgcagtacc gctccccata ataatcgtgg
      481 tgacattgtc tgcagcgcca gctggcttta cgcaccacgg gtaaggcatc cgggacgaat
      541 tgctgcagac gtttaatcaa ccgtatttct ctgcgttccg gcctgacata aggacattgc
      601 tgcgcgtgct ccgtcagccc ttcgtctgcg tgttcaaacc agggacgctc agtgtcgtat
      661 tgcgggtggt atctgagcgc actgccgcaa aggtggcaag tgtagcgatc gtaaggtgca
      721 gtctgtgcgg tacgggcacc cgtcagacgc ccgttgccat caaatgcaat aaaggatttt
      781 gcgtacatcg tcatattcct cagccgccag acgacacgca ggcaacagct gtcccggtac
      841 gggcagtgtg gtcagggtgt gaatggcggt tcagttaaaa tctggactgg agtttgggta
      901 aaacaggctg gttgctgtta ccgcactgtg gttgtcacac aatggcccac ctgcgtaaaa
      961 aactgtcagc aggcagggat ggacatcggg ggatgtgctg tatcggttgc cggaaggaat
     1021 ggtaaagaat gtgccatggt taaatctccg tatggttcat acggaatagc catgtcgtaa
     1081 aaaatgcgca gagtccctga agtggcctcc ggaaccgact gt
//
LOCUS       DAZFLD010000532         1118 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.535, whole genome shotgun sequence.
ACCESSION   DAZFLD010000532 DAZFLD010000000
VERSION     DAZFLD010000532.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1118)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1118
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.535"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..839)
                     /locus_tag="ACHKBL_005184"
     CDS             complement(<1..839)
                     /locus_tag="ACHKBL_005184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001451800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ash family protein"
                     /protein_id="HFO5821885.1"
                     /translation="MTHKTKATTKGSPVEIKEIKCITNYAMSVAGGQGYQPGGRFLFA
                     FNRRGYFSTKSSRANFLAFVCDAFDSSSHVFPRLILFSIKREAAFLFSDESMGLLPVC
                     CSTMASMSSWCCSCRKNFSSSGGKSILISCTAERRSTSHHGADGCYSFYGVREAKADR
                     YRFLGEINRRGYLSRNASSANRFGLVRGEGLSKDSRSRRTFSRWRSSMDNAISCNSSF
                     VIVCTRKAVRCSTSYHSAGIFYGCNLRDDSGRFATHCININNVMLLCAFKENTGIFRT
                     NFSR"
     gene            complement(850..1059)
                     /locus_tag="ACHKBL_005185"
     CDS             complement(850..1059)
                     /locus_tag="ACHKBL_005185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001206970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO5821886.1"
                     /translation="MREINEDRVIREDECRKLTGVCRTTRYELEKKGCFPSRLNLGGR
                     SVGWLLSEVMEWVKSRDRINSGKAA"
BASE COUNT          338 a          279 c          213 g          288 t
ORIGIN      
        1 cgagagaagt ttgtgcgaaa aatacctgtg ttttctttga aagcacataa taacatcacg
       61 ttatttatgt ttatgcaatg tgtcgcaaag cgacctgaat catcacgcaa attacaacca
      121 taaaaaatac ctgcgctgtg atatgaggta ctacagcgca ccgctttacg ggtacagaca
      181 atgacaaatg aagaattaca ggaaatcgca ttatccatac ttgagcgcca gcgcgaaaaa
      241 gtacgcctgg aacgcgagtc tttgcttaat ccctctcctc tcaccagacc aaaacgattt
      301 gcgcttgagg catttctgga caaataccca cggcgattaa tctcgcctaa aaaccgatag
      361 cgatccgctt ttgcttctct gactccataa aacgaataac aaccatcagc gccgtggtgt
      421 gaggtgctgc gtcgctccgc tgtacaggaa ataagtatgg atttaccacc agaagatgag
      481 aaatttttac gacaactaca acaccaacta ctcatggatg ccattgtcga gcagcaaaca
      541 gggagcagcc ccattgattc atcgctgaat aaaaaggccg cctcccgttt tattgaaaac
      601 aggataaggc gcggaaaaac atgtgatgac gagtcaaagg catcacatac aaaagctaaa
      661 aaatttgccc ttgaagattt tgtcgagaaa tacccacggc ggttaaaagc gaataaaaac
      721 cgaccacctg gctgatagcc ttgtccacca gctacactca tggcatagtt ggtgatgcac
      781 ttgatttcct ttatctccac tggcgaccct ttcgtggtcg cctttgtttt atgtgtcata
      841 tgctcccctt tacgctgcct tgcctgaatt aatgcgatcc ctgcttttaa cccattccat
      901 aacctcggac agcagccaac ctacagaacg accacccaga ttaagacgtg acggaaagca
      961 tccttttttc tccagttcgt agcgtgtagt gcgacacact ccagttaact tacgacattc
     1021 atcctcacgg attacgcgat cttcatttat ttcacgcata caaaaaccta cataaaaatt
     1081 acgtatataa acttttctct tagctgtaga tatatgat
//
LOCUS       DAZFLD010000533         1114 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.536, whole genome shotgun sequence.
ACCESSION   DAZFLD010000533 DAZFLD010000000
VERSION     DAZFLD010000533.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1114
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.536"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            2..>1114
                     /locus_tag="ACHKBL_005186"
     CDS             2..>1114
                     /locus_tag="ACHKBL_005186"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HFO5821887.1"
                     /translation="MSIYSRKQCTLEDAVNMIHDNDCVILGGFIGAVVPECIEKGIED
                     RFLKTGKPRNLKIIFAAGQGDAGTKAVNHLSHEGLVTSAIGGHWGLIPKLQKLANEGK
                     ITGYNLPQGVICHLFRDSAAGKLGTLTHVGLGTFVDPRVEGGKINDKTQEDIVSVTEI
                     NGVENLFYKKIDANIAILRGTTSDQSGNITMEDECLILENLAAAQLVHNNGGKVIVQV
                     KQIVRDGTLDPLQVRIPGIYVDAVVVSEDEYHMQTFAEYMNFSYVGKGVKEVKDKETR
                     PLDIKKIIARRAAMELKKNAVVNYGIGAPEVIAEVTDEEGITSELIATVEPGAIGGTP
                     AGGLSFGASSFPQAIITQDQMFDFYDGGGLDQAFLGL"
BASE COUNT          349 a          169 c          272 g          324 t
ORIGIN      
        1 tatgtctatt tattcaagaa aacaatgtac actagaagat gccgtaaata tgattcatga
       61 taatgattgt gttattcttg gcggatttat tggtgcagtt gttcctgagt gtatagaaaa
      121 aggcatagaa gatcgttttc tgaaaacggg aaaaccaaga aatctcaaga ttatttttgc
      181 tgctggtcaa ggggatgcag gaacaaaagc tgtcaatcat ttatctcatg aggggcttgt
      241 cactagtgcc attggcgggc attggggact aatacctaaa ttgcagaagt tagctaatga
      301 gggaaaaatt acgggttata atcttcctca aggggttatt tgccatttat tccgagatag
      361 tgctgctggg aagttaggga cattaactca tgttggttta ggtacatttg ttgatccccg
      421 cgtagagggc gggaaaatta atgataaaac acaagaggat attgtgtccg tcacagaaat
      481 taatggtgtg gaaaacctat tttataagaa gattgatgct aacatagcaa tcctcagagg
      541 tactacatct gatcagagtg ggaatataac aatggaggat gaatgtttga ttcttgaaaa
      601 cctggctgct gcccagttag tccataataa tggagggaaa gttattgttc aggtaaaaca
      661 aatagtacgt gacggtacat tagatccttt acaagtcaga attccaggta tttatgttga
      721 tgctgttgtt gtttctgaag atgaatatca tatgcaaact tttgcggagt atatgaactt
      781 cagctatgtc gggaaaggtg taaaagaggt aaaagacaaa gaaacacgcc ctcttgatat
      841 aaaaaaaatt atagctcgac gagccgcaat ggaactgaaa aaaaatgcag ttgtaaatta
      901 tggcataggg gcacctgaag ttatagctga agtaacagat gaagagggga ttacctccga
      961 acttatcgca actgttgaac caggtgcaat tggaggaacg cctgctggag gtctgtcatt
     1021 tggagcttct tcgttccctc aggcgataat tactcaggat cagatgtttg atttctatga
     1081 cggtggtggg cttgatcaag cgtttcttgg atta
//
LOCUS       DAZFLD010000534         1114 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.537, whole genome shotgun sequence.
ACCESSION   DAZFLD010000534 DAZFLD010000000
VERSION     DAZFLD010000534.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1114
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.537"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1020
                     /locus_tag="ACHKBL_005187"
     CDS             <1..1020
                     /locus_tag="ACHKBL_005187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DotA/TraY family protein"
                     /protein_id="HFO5821888.1"
                     /translation="FLKEVKGAYQAQLQNSPYTPALGNLTSSDEIYAANTNDPQSAII
                     KLMSPDFTKLTYKIATVYSSTGTTSYQANPLLKMKDIGDYTMVTTETIWAGYTTVRVL
                     STAGSNSIFGRLVNVVSGLSDAAKAFLEAAAPPLYFLLFLLFCAGFSLSIYLPFIPFI
                     FWMTGIGNWIVSVLIGCAAGPLWAATHLGTSQDRGSRAAYGYIYLIDSMIRPLLMVFG
                     FFFASVAIVAGGTILNALFAAALMNVQVSSVTGVFSVAGFLMIYARACTTLVAGVFAL
                     QAYLPDHVINFLGGRDGVNMLGNMVSSVKDIIAGGNTNIRRTPGVREEKLKNINRDDK
                     DGIKQ"
BASE COUNT          295 a          247 c          269 g          303 t
ORIGIN      
        1 ttcctgaagg aagtcaaagg agcttaccag gcacagttac agaactcacc atatactcct
       61 gcgctgggaa atctaacatc ttctgatgag atatatgctg ccaatacaaa tgatccacaa
      121 agtgcaatta taaagttaat gtcaccggac tttacgaaac tgacatataa aatcgcaact
      181 gtatattcta gtacaggaac tacatcctat caggcaaatc ccctgctgaa gatgaaggat
      241 atcggtgatt acacaatggt aacaaccgag accatctggg cgggttatac caccgtcagg
      301 gtgctttcca ctgccggcag caactccatt tttggtcgac tggtcaatgt cgttagtgga
      361 ttatctgacg ccgcaaaagc ttttctggaa gctgctgcac caccgctcta cttcctgctg
      421 tttttgctgt tctgtgccgg tttttcgtta tccatttacc tgccgtttat tccgtttatc
      481 ttctggatga ccggcatcgg caactggata gtcagcgtgc tgattggatg tgccgccggc
      541 cccctgtggg ctgccaccca tctgggaaca agccaggacc ggggaagccg ggctgcttac
      601 ggttacatct acctgataga cagcatgatc aggcctctgt tgatggtttt cgggttcttt
      661 tttgcatcgg tggccatcgt ggccggcggc acaatactga atgcgctgtt tgctgctgcg
      721 ctgatgaatg tccaagtaag ttctgtaaca ggcgtgttca gcgtggcggg atttttgatg
      781 atttatgccc gcgcatgtac cacactggtt gcgggcgttt ttgcgcttca ggcttatctg
      841 ccagaccatg tgattaattt cctgggcgga cgtgacggag ttaatatgct ggggaatatg
      901 gtttcttccg tcaaagatat tattgccggc ggaaacacca atatacgcag aacacctggc
      961 gttcgtgaag agaaactaaa aaatattaac agggatgata aggatggaat aaaacaatag
     1021 agtaaaaaaa tcaaaaacaa ataaaaagca ttgccatatt cgcctggtga tattaaaata
     1081 caaccaggtg aatatggagg tttattatga atga
//
LOCUS       DAZFLD010000535         1106 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.538, whole genome shotgun sequence.
ACCESSION   DAZFLD010000535 DAZFLD010000000
VERSION     DAZFLD010000535.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1106
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.538"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            48..>1106
                     /locus_tag="ACHKBL_005188"
     CDS             48..>1106
                     /locus_tag="ACHKBL_005188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5821889.1"
                     /translation="MSGFAQGLLAGFSTVDQAMTRRKELGLREAQLAQQQKNNERDFE
                     FAQSQFEHNKDVDQRNFDYRAKVDDRNYALQEREFNANQNYRNASLGMEQQRLQLQKY
                     NQRRLEYNDMIAHSQPLMEALGKAIEAGDQEAATRLFGQLPKGHPLILMSNEGYAAKA
                     GQAVINLQKIFGDKPDMAIDSLNTPENLDVLSGVFAPELQQRIGMPDSTGEKTIKEAR
                     IGSIVPAQQEGYVLIGLDLTYSDGSTAHKPVTEYGSAHPDDQTVLAIPVDKAIAQVRD
                     RSKFAEISKNYGYFMPKQQGLSLKELQKEASKVVAQAIKDGRDAQAANDEFLAAYDSL
                     PHQQKVQQRKTQQLLQKWQ"
BASE COUNT          320 a          244 c          303 g          239 t
ORIGIN      
        1 tgcagtaatt ggcggcattg caggcgcttt gttttaagga gtggtgaatg agcggatttg
       61 cacaggggtt acttgccgga ttcagcactg ttgaccaggc aatgacccgt cgtaaggagc
      121 ttggtttgcg agaagcacag cttgctcagc aacagaaaaa taacgagcgc gattttgaat
      181 ttgcgcagtc tcagtttgaa cataataaag acgttgatca gcggaacttt gattacagag
      241 ccaaagttga tgaccgcaat tacgcactgc aggaaaggga gtttaacgcc aaccagaatt
      301 accggaatgc gtcgctgggg atggaacagc aacgactcca gttgcagaaa tacaaccagc
      361 gacggcttga gtataacgat atgattgccc atagccagcc actaatggaa gcgcttggaa
      421 aagcaattga ggctggcgat caggaggctg caacgcgtct gttcgggcag ctgccaaagg
      481 gacatccatt aattcttatg tcaaacgaag gctatgcagc gaaagcgggt caggccgtga
      541 tcaacctgca aaaaatcttt ggtgataagc cggacatggc gatcgattcg ctgaataccc
      601 cggaaaatct cgatgtgctt tccggcgtgt ttgccccgga actacaacag cgtattggca
      661 tgcctgattc aaccggggaa aaaacgataa aagaggccag gattggcagt atcgtaccag
      721 cgcagcagga agggtacgta cttattggcc ttgatctcac atacagcgat ggctccaccg
      781 cgcataaacc tgtaacagaa tacggcagtg cgcaccctga tgatcaaacc gtgctggcga
      841 tacccgttga taaggctatc gctcaggtca gggatcgcag caaatttgca gagatatcga
      901 aaaattatgg ttattttatg ccgaagcagc agggactttc tctgaaagag cttcagaagg
      961 aagccagcaa agtagtggct caggcgatca aggatggccg tgatgctcag gctgcgaatg
     1021 atgagttttt agcagcgtat gattcactgc cgcatcaaca gaaagttcag caacggaaaa
     1081 cgcaacagct tttacagaag tggcag
//
LOCUS       DAZFLD010000536         1101 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.539, whole genome shotgun sequence.
ACCESSION   DAZFLD010000536 DAZFLD010000000
VERSION     DAZFLD010000536.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1101)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1101)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1101
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.539"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            561..953
                     /locus_tag="ACHKBL_005189"
     CDS             561..953
                     /locus_tag="ACHKBL_005189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001694413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HFO5821890.1"
                     /translation="MRDIQQVLERWGAWAANNRENVTWSSVAAGFKGIIPAKITSRSQ
                     CCDDDAMVICGCIARLYRNNRDLHDLLVDYYVLGETFMALARKHGCSDTCIGKRLHKA
                     EGVVEGMLMMLDVKLEMDRYVERDSVTA"
BASE COUNT          304 a          235 c          298 g          264 t
ORIGIN      
        1 ctggcgcggt gtacacggtg gaacgcatag cgttacctga cttattgcat tgtaaaaagt
       61 tagaaaggca cggcctgtaa gatgggatgt tgtgagtagt ttaaagctac ccgcaaattc
      121 tttcgttgtg ctggcacagg cggttaaacc gtcggtaact ggataatccc cattggggga
      181 aaagctggct acacccctca catctgagga ctgataacgc aaaattgcgt tggctggcag
      241 agtgcaaatt tgcacgatgg cctaacccat tgattctttc gaaaaccccc attgggggag
      301 aacgggggaa taaacctgac tcattgattc ttccacaatc ctcaatttga ggaagtcggc
      361 gcggtaactc actgagatta aagcaaagct caaatctgag atggctacat ccctcacatc
      421 tgaggactgt taaccagcaa gccggaatag attcacaaac tacgaaattt tcgtagttta
      481 actttcatcc agtacatccc cgaaaacacg ggtatgtacg gagttaccgt taccaataat
      541 tattcgcgag gtgattgttg atgcgtgata ttcagcaggt tctggaacgc tggggagcgt
      601 gggcggcaaa taatcgcgag aatgtgacct ggtcatccgt tgccgcagga tttaagggga
      661 ttattccggc aaagataaca tcacgctcac agtgctgtga cgatgatgcg atggtgatat
      721 gcgggtgcat agcccgtctt taccggaaca atcgcgatct gcatgacttg ctggttgatt
      781 attacgtgtt gggggagacg ttcatggcgc tggcacggaa acatgggtgc tctgacacct
      841 gtataggtaa acgccttcac aaagcggagg gggttgttga aggcatgttg atgatgctgg
      901 atgtgaagct ggagatggat cggtatgttg agcgcgattc agtgacggca taacgcgcat
      961 aaaaaaaccg cagtcatgag gaaactgcgg tggagaaata cggcttgcca atcaatcatg
     1021 aaaaacgtaa tcgcctgata attatgccac gagacaaagg acggtgcacc ctgaatggtc
     1081 ataatgacca ctcagaccat c
//
LOCUS       DAZFLD010000537         1097 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.540, whole genome shotgun sequence.
ACCESSION   DAZFLD010000537 DAZFLD010000000
VERSION     DAZFLD010000537.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1097)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1097
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.540"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..325
                     /locus_tag="ACHKBL_005190"
     CDS             <1..325
                     /locus_tag="ACHKBL_005190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFO5821891.1"
                     /translation="AQYDAYMVLWSRINELLTEPDIIAFINYPIAHSLMHLNDDEIHG
                     VRPEEFPDEEMKIKWITGWHAEYQHFTDNLPLHLRERIMVCAHPDDIDDFSDKVIQKA
                     MMLRT"
     gene            327..752
                     /gene="nudI"
                     /locus_tag="ACHKBL_005191"
     CDS             327..752
                     /gene="nudI"
                     /locus_tag="ACHKBL_005191"
                     /EC_number="3.6.1.12"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020883888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HFO5821892.1"
                     /translation="MRQRVIVCPVIQNEGEFLLCKMASDRGVFPGQWALPGGGMEPGE
                     TMETALRREIKEELGKELHITDVKPWAFRDDIRTKTYPDGTHEDIYMIYLIFDCISTN
                     RTVTFNEEFQDIVWASPDTLKYMDLNEATRITFSQKGIL"
BASE COUNT          343 a          222 c          243 g          289 t
ORIGIN      
        1 agcacaatat gatgcataca tggtgctctg gagtagaata aatgaattac ttacagagcc
       61 agacattata gcattcatta attatcccat cgcacattca ctcatgcatc tcaatgatga
      121 tgaaattcat ggtgtacggc ctgaagaatt tccagatgag gaaatgaaga taaaatggat
      181 cacaggatgg catgcggaat atcaacattt tactgataat cttccgttac accttcgtga
      241 gcggattatg gtatgtgctc acccggatga cattgatgat ttttctgata aggttatcca
      301 aaaagccatg atgttgagga cataaaatgc gccagagagt aatagtgtgc cctgtgatac
      361 aaaatgaagg tgagtttcta ctttgtaaaa tggcctctga tcgcggagta ttcccgggcc
      421 aatgggcact gcccggtgga ggaatggaac ccggagagac tatggaaaca gcattgaggc
      481 gtgaaataaa ggaagaactg ggaaaagaac tgcatatcac tgacgtaaaa ccctgggcct
      541 ttcgtgatga tatccgtaca aaaacgtacc cggatggtac ccacgaggac atttacatga
      601 tttacctaat attcgactgt atcagtacaa accgtacagt tacatttaat gaggagtttc
      661 aggatatagt ctgggcatct cctgacaccc taaaatatat ggatttaaat gaagcaaccc
      721 ggatcacatt ttctcagaaa ggtatccttt aaactccaat gctaatcgtg caactgataa
      781 cggcttccac aacagggagc cgttttctta tgccactact caaggatggg gtttgagggc
      841 caaccgtacg aaaacgtaca attctgtaca tctacgcaag gaaatgtacg gaagtaagcc
      901 aaatggttat cggacggtcg gaacatttcg tacagtcttt acgttcagtg agttagctgg
      961 cctggatgta accactcgtt caactcaatt ctcttagcgt gggaaaattt ccgaattcca
     1021 agcggacccc ttattgtacg ttcaacgcac catcattaat gctaatgtat gcatacatta
     1081 gcattaatga tgaagga
//
LOCUS       DAZFLD010000538         1082 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.541, whole genome shotgun sequence.
ACCESSION   DAZFLD010000538 DAZFLD010000000
VERSION     DAZFLD010000538.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1082)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1082)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1082
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.541"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            3..>1082
                     /locus_tag="ACHKBL_005192"
     CDS             3..>1082
                     /locus_tag="ACHKBL_005192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFO5821893.1"
                     /translation="MKKAIAYMRFSSPGQMSGDSLNRQRRLIAEWLKVNSDYYLDTIT
                     YEDLGLSAFKGKHAQSGAFSEFLDAIEHGYILPGTTLLVESLDRLSREKVGEAIERLK
                     LILNHGIDVITLCDNTVYNIDSLNEPYSLIKAILIAQRANEESEIKSSRVKLSWKKKR
                     QDALESGTIMTASCPRWLSLDDKRTAFVPDPDRVKTIELIFKLRMERRSLNAIAKYLN
                     DHAVKNFSGKESAWGPSVIEKLLANKALIGICVPSYRARGKGISEIAGYYPRVISDDL
                     FYAVQEIRLAPFGISNSSKNPMLINLLRTVMKCEACGNTMIVHAVSGSLHGYYVCPMR
                     RLHRCDRPSIKRDLVDYNIINELLFN"
BASE COUNT          362 a          162 c          239 g          319 t
ORIGIN      
        1 ccatgaaaaa agccatagca tatatgcgat tttcatcacc aggtcagatg tctggcgact
       61 cattaaaccg acagagaaga cttattgctg aatggttaaa ggtaaatagt gattattatc
      121 ttgataccat aacatatgaa gatttaggat taagtgcatt caaaggaaag catgcacaat
      181 caggagcttt ttcggaattt ttagatgcta tagagcatgg ttatatattg ccaggaacta
      241 cattgttagt tgaaagtctg gacagacttt caagagaaaa agtcggtgaa gcgattgaac
      301 gtctgaaatt gattttgaat cacggtattg atgttataac tctttgcgat aatacagtct
      361 ataatattga ctctttgaat gagccatatt cattaataaa agccatactt atagcacaaa
      421 gggcaaatga agaaagcgag ataaagtcaa gtcgggttaa attatcatgg aagaaaaaac
      481 ggcaggatgc actggaatca ggtacgatta tgacggcgtc ttgtccgaga tggctctcct
      541 tagatgacaa aagaacggct tttgttccag accccgacag ggtgaaaact attgagctaa
      601 tttttaaact caggatggaa aggcgctcat tgaatgcaat agccaagtat ttaaatgatc
      661 atgctgtaaa gaatttctca ggaaaagaaa gtgcatgggg accttctgta attgaaaaat
      721 tattagcgaa taaagctctg ataggtatat gcgtaccttc atatcgtgca agagggaaag
      781 ggataagtga aatcgctggc tattatccca gagtcatatc agatgatttg ttttacgctg
      841 tacaggaaat tcggttggca ccttttggta ttagcaatag tagcaagaat cctatgctaa
      901 taaatctact tcgaacagtt atgaagtgtg aggcttgtgg taataccatg attgttcatg
      961 cggtatctgg aagtttgcat ggctattatg tttgtccgat gagaagatta catcgatgtg
     1021 acaggccatc aataaaaaga gatttggttg attataatat cattaatgaa ttgcttttta
     1081 at
//
LOCUS       DAZFLD010000539         1078 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.542, whole genome shotgun sequence.
ACCESSION   DAZFLD010000539 DAZFLD010000000
VERSION     DAZFLD010000539.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1078)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1078)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1078
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.542"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..430
                     /locus_tag="ACHKBL_005193"
     CDS             <1..430
                     /locus_tag="ACHKBL_005193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009404 - toxin metabolic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="toxin-activating lysine-acyltransferase"
                     /protein_id="HFO5821894.1"
                     /translation="RGKDEWTVYEQIGFAVSCMLYNRNYSLYPVLTIQYWTEYAIQHN
                     QIKFLFDSRGFPLAYITWAYLEADTEARLLRDPEFRLHPSEWNEDGRIWILDFCCKPG
                     FGRKVIDYLIQLQPWGEGEVRWLSRRKKIVTFIPERLHKT"
     gene            583..>1078
                     /locus_tag="ACHKBL_005194"
     CDS             583..>1078
                     /locus_tag="ACHKBL_005194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000489618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V secretion protein CvaA"
                     /protein_id="HFO5821895.1"
                     /translation="MFRQDALENRKMKWQGRAILLPGIPLWLIMLGSIVFITAFLMFI
                     IVGTYSRRVNVSGEVTTWPRAVNIYSGVQGFVVRQFVHEGQLIKKGDPVYLIDISKST
                     RSGIVTDNHRRDIENQLVRVDNIISRLEESKKITLDTLEKQRLQYTDAFRRSSDIIQR
                     AEEGI"
BASE COUNT          323 a          183 c          265 g          307 t
ORIGIN      
        1 cagaggaaaa gatgaatgga ctgtttatga acagatcggt tttgcggtca gttgtatgct
       61 ctacaatcgt aattacagtc tgtatccggt gttaaccatt caatactgga ctgaatatgc
      121 gatacagcat aatcagatta aattcctgtt tgattcacga ggttttccac tggcgtatat
      181 tacctgggca tatcttgagg ctgatacaga agcgcgcctg ctcagggatc cagaattcag
      241 gttgcatccg tctgaatgga atgaagatgg aaggatttgg attctggatt tctgttgtaa
      301 accaggcttt ggtcgaaaag ttattgacta tctcatacag cttcagccat ggggagaagg
      361 agaagtacga tggttaagca ggcgaaagaa aattgtgaca ttcatccctg agcggctgca
      421 taaaacgtag tacctctgaa gatacagaga taattgtaaa ttacggggta aatgcatcgc
      481 tgatactatt ttgacaggac tctgtatttt ctggctttga ttatagcatt tcgcttaacc
      541 agtgaaggta ttacaggaaa agtgtgagta agaggagcta ttttgtttcg tcaggatgct
      601 ttagaaaaca gaaaaatgaa gtggcaggga cgggcaatat tacttcccgg aataccacta
      661 tggttaatca tgctgggaag catagtgttt attacggcat ttctgatgtt cattattgtt
      721 ggtacctata gccgccgtgt taatgtcagt ggtgaggtca caacctggcc aagagctgtc
      781 aatatatatt caggtgtaca gggatttgtt gtcaggcagt ttgttcatga aggacagttg
      841 ataaaaaaag gggaccctgt ttatctgatt gacatcagta aaagtacacg tagtggtatt
      901 gtcactgata atcatcgtcg ggatatagaa aaccagctgg ttcgtgtgga caacattatt
      961 tcccgtctgg aagaaagtaa aaaaataacg ctagataccc tggaaaaaca acgcctgcaa
     1021 tacacagatg cgtttcgtcg ctcatcagat attatacagc gtgcagagga agggataa
//
LOCUS       DAZFLD010000540         1072 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.543, whole genome shotgun sequence.
ACCESSION   DAZFLD010000540 DAZFLD010000000
VERSION     DAZFLD010000540.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1072)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1072)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1072
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.543"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(78..1022)
                     /gene="leuO"
                     /locus_tag="ACHKBL_005195"
     CDS             complement(78..1022)
                     /gene="leuO"
                     /locus_tag="ACHKBL_005195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LeuO"
                     /protein_id="HFO5821896.1"
                     /translation="MPEVQTDHPETAELSKPQLRMVDLNLLTVFDAVMQEQNITRAAH
                     VLGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPG
                     SGFEPASSERVFHLCVCSPLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQ
                     ETEFVISYEDFHRPEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDR
                     FASFSQTWYDTVDKQASIAYQGMAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVL
                     PLPLKQNSRTCYLSWHEAAGRDKGHQWMEEQLVSICKR"
BASE COUNT          302 a          254 c          224 g          292 t
ORIGIN      
        1 attcagaata aaattctata ttcaataaaa aatgcagaat aaaccagaca ttaatgtctg
       61 acctattctg cattcagtta gcgtttgcaa attgagacta attgctcttc catccactga
      121 tggcctttat cgcgcccggc agcttcatgc caggagagat aacaggttct gctgttttgt
      181 tttaacggca gcggtaatac ctgtaactct aaggattcag cgaactcttc agccagccaa
      241 cgcggcgcaa tagcgaccag atgcgtttgc gacaccacgc taagtacgct catcattgcc
      301 atgccctgat acgcgatact ggcttgctta tctaccgtgt cataccaggt ttgactaaat
      361 gacgcgaaac gatcgagcga aaccgcagca tgttgttcgt tataaacatc atgtttcagt
      421 aacgggcctt taattgttgg atgatttttg ctggctacca acaccatttc atctttaaat
      481 aatggcacac tggtaaattc agggcgatgg aagtcttcat aactaatcac aaactccgtt
      541 tcctgataac gcagctgatg ttcagtgttc tgatttaatg aagacttgaa cataacatgt
      601 atatttggcg caatctgctc aatgtgatta taaatctgcg aggtcagaat gctgtctaac
      661 gggctgcaaa cacaaagatg aaatacacgt tcactgctcg cgggttcaaa acctgaacca
      721 ggcaattcat tttgtactag ttgcaatgcc tgacgaactg agccaaaaag ttgaaatgcg
      781 cgagcagtcg gttgaatacc acggccataa cgaacaaaaa gctcgtcatt aaacatcacc
      841 ttcaggcgtg caacagcgtt actgaccgca ggttgcgaca ttcccagaac atgagcggca
      901 cgggtgatgt tttgctcctg catcacggca tcgaaaacgg ttaataagtt gagatcgacc
      961 atgcgtagct gtggcttgct taactccgcc gtctctggat gatctgtttg aacctctggc
     1021 atacttaact ccactgtcac acttaactcc ctttccctta ttggaatgca ga
//
LOCUS       DAZFLD010000541         1071 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.544, whole genome shotgun sequence.
ACCESSION   DAZFLD010000541 DAZFLD010000000
VERSION     DAZFLD010000541.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1071
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.544"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            177..644
                     /locus_tag="ACHKBL_005196"
     CDS             177..644
                     /locus_tag="ACHKBL_005196"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5821897.1"
                     /translation="MKGKIIPEKNKIKNLINQERDAIALWIKDGMRLPDIYRLLKEKH
                     PELTFSQNGFVFSLRRDASDLHENALFNRSTVAVFMQKQHSEMTLMVNSGCLLKNVHE
                     ALFSHISYSVFLRYIVKLYPDLHYQAKLNRKGAVCGELHHEENASVYQNDEVC"
     gene            complement(767..>1071)
                     /locus_tag="ACHKBL_005197"
     CDS             complement(767..>1071)
                     /locus_tag="ACHKBL_005197"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5821898.1"
                     /translation="LSYENTHVKTNKKGTTWHIFYRENSGAEVLLEIPSFRECLSVSK
                     ELMTPSNYMICIEKNGERIKRWDREIIAGSNKWINCPPDNFEILGELITINRIIKK"
BASE COUNT          305 a          169 c          225 g          372 t
ORIGIN      
        1 tggcagcctc atgtatttat gtggagatta tttcatcgga tattatttta tccaggaatg
       61 ttgttttgtt aaagtgagta agtcttatag attatgtttc tttactgaat tccgggtatg
      121 tagacaaagc tggttctgta tatgtgaact tctttgttgt tcctgaggga gataatatga
      181 aaggcaagat tataccagaa aaaaataaga taaaaaactt aatcaaccag gagcgtgacg
      241 ccattgcact ctggattaag gatggaatga gactgccaga tatttacaga ttactgaagg
      301 aaaaacaccc ggaactgacg ttttcacaga atggttttgt attttcactg agacgtgatg
      361 caagtgattt acatgaaaat gcgcttttta atcgcagtac tgttgctgta tttatgcaga
      421 aacaacattc tgagatgact ctcatggtta attctggttg tttgctgaaa aatgtgcatg
      481 aggcgttatt cagtcatata tcttactccg tgtttcttcg atatattgtg aagttatatc
      541 cagatttaca ctatcaggca aaattaaaca ggaaaggggc tgtctgtggt gaattacatc
      601 atgaggagaa tgcttctgtt tatcagaatg acgaggtttg ttaaaggtgt tttataaaaa
      661 tagtggggta gtgctggcac agagatgtct tacaggcatt ctcccgcaaa aaatgaaccc
      721 gttgcttgtt atagtgcctt gcttaataca ttgcctgaat ccggagttac tttttaataa
      781 ttctgttaat ggttattagc tcacctaata tttcgaagtt atcaggtggg cagttaatcc
      841 atttgttaga gccagcgatg atttccctgt cccagcgttt tattctttcg ccattttttt
      901 ctatgcatat catgtaattt gatggggtca ttaattcttt ggatactgaa agacattccc
      961 gaaatgatgg tatttcaagc aaaacttccg cgcctgaatt ttcacggtaa aaaatgtgcc
     1021 atgttgtgcc ttttttatta gttttcacgt gcgtattctc ataggataaa t
//
LOCUS       DAZFLD010000542         1070 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.545, whole genome shotgun sequence.
ACCESSION   DAZFLD010000542 DAZFLD010000000
VERSION     DAZFLD010000542.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1070
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.545"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>1070
                     /locus_tag="ACHKBL_005198"
     CDS             <1..>1070
                     /locus_tag="ACHKBL_005198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000431134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFO5821899.1"
                     /translation="RLNHYCARALEGAASLCQTRAHAEITPEHWLLKLLEQGEGDLTV
                     LGRRYDWDMDAIWQSLLGWLDNQPRSVRSRPQLAPSLNALLKQAWMVASLQGEEHIRS
                     VHLLGALTENPHLVRCDGVWPLLTLSQSQLQRLSPLLDAQSDECPERLQDAEPVLPQG
                     DNVTFIGRPVGADTAGIPSGDLPPVLQSALDKFTRYITASAREGKIDPVSGRDTEIRQ
                     MVDILSRRRKNNPILVGDPGVGKTALVEGLALRIAEGNVPESLRPVTLRTLDLGLLQA
                     GAGVKGEFEQRLKNVIDAVQQSPAPVLLFIDEAHTLIGAGNQAGGADAANLLKPALAR
                     GELRTIAATTWSEYRQYLERDAA"
BASE COUNT          215 a          306 c          340 g          209 t
ORIGIN      
        1 cgtcttaatc attactgtgc ccgtgcactg gaaggcgcag cctccctttg ccagacccgg
       61 gcccatgcag aaatcacccc ggagcactgg ttactgaaac tgctggagca gggggaagga
      121 gaccttaccg tgctgggccg gcgttatgac tgggatatgg acgcgatatg gcagtcgctg
      181 ctcggctggc tggataacca gccccgtagc gtacgcagcc gcccgcagct tgcgccgtcc
      241 ctgaatgccc tgctgaaaca ggcctggatg gtggcctcat tacagggaga agaacatatc
      301 cgcagcgtgc atctgctggg tgccctgacg gaaaatccgc acctggttcg ctgtgacggg
      361 gtgtggcccc tgctgacact gagtcagagc cagttgcagc gtctgtcccc gctgctggat
      421 gcgcagtctg atgagtgtcc ggagagatta caggatgcag agcctgtgct gcctcaggga
      481 gataatgtga cctttatcgg gcgtcccgtc ggtgcggata cggcgggtat accgtcaggt
      541 gacctgccgc ctgtgttaca gagtgcgctg gataaattca cccggtacat cacggccagc
      601 gcgagagagg ggaaaattga cccggtatcg ggacgcgata cggaaatccg tcagatggtg
      661 gatattctct cccgccgccg caagaacaac ccgattctgg tgggggaccc tggtgtgggg
      721 aaaacggctc tggtggaagg gctcgccctg cgtattgcgg aaggtaacgt accggaatca
      781 ctcagacccg tcaccctgcg cacccttgac ctcggcctgc tgcaggccgg tgccggcgtg
      841 aaaggtgaat ttgaacagcg cctgaaaaac gtgattgatg ccgtgcagca gtcaccggct
      901 cctgtactgc tgtttataga tgaagcccat acccttatcg gtgccggtaa tcaggccggt
      961 ggcgcggatg cggccaacct gctgaagcct gcgctggcgc ggggtgaact gcgcaccatt
     1021 gccgccacca cctggtctga gtacagacaa tacctggaac gtgacgcagc
//
LOCUS       DAZFLD010000543         1065 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.546, whole genome shotgun sequence.
ACCESSION   DAZFLD010000543 DAZFLD010000000
VERSION     DAZFLD010000543.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1065
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.546"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(55..1029)
                     /gene="cysB"
                     /locus_tag="ACHKBL_005199"
     CDS             complement(55..1029)
                     /gene="cysB"
                     /locus_tag="ACHKBL_005199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HFO5821900.1"
                     /translation="MKLQQLRYIVEVVNHNLNVSSTAEGLYTSQPGISKQVRMLEDEL
                     GIQIFSRSGKHLTQVTPAGQEIIRIAREVLSKVDAIKSVAGEHTWPDKGSLYIATTHT
                     QARYALPNVIKGFIERYPRVSLHMHQGSPTQIADAVSKGNADFAIATEALHLYEDLVM
                     LPCYHWNRAIVVTPDHPLAGKKAITIEELAQYPLVTYTFGFTGRSELDTAFNRAGLTP
                     RIVFTATDADVIKTYVRLGLGVGVIASMAVDPVADPDLVRVDAHDIFSHSTTKIGFRR
                     STFLRSYMYDFIQRFAPHLTRDVVDAAVALRSNEEIEIMFKDIKLPEK"
BASE COUNT          276 a          259 c          256 g          274 t
ORIGIN      
        1 gaatatattc cggcaccttc gctacataaa aggtgccgaa aataacgcaa gaaattattt
       61 ttccggcagt tttatatctt taaacatgat ctcaatttct tcattagagc gtaatgcgac
      121 agccgcatca acgacatcac gcgttaaatg cggtgcaaaa cgctgaatga aatcatacat
      181 ataactgcgc aaaaaagtac tacggcgaaa accaattttg gttgtactgt ggctgaagat
      241 atcgtgagca tcaacgcgca ccaggtcggg atcggcgacc ggatccaccg ccatgctggc
      301 aatgacccct acccccagcc ctaaccggac gtaagtttta atgacgtcag catccgttgc
      361 cgtgaagacg atacgcggcg ttaaccctgc gcggttaaag gcagtatcca gctctgaacg
      421 tccggtaaag ccgaaggtat atgtcaccaa cggatattgc gccagttctt caatggtaat
      481 ggcttttttg cctgccagcg ggtgatccgg agtgactaca atagcccgat tccagtggta
      541 gcacggtaac atcactagat cttcatacag atgcaacgct tctgtggcga tagcgaaatc
      601 agcattgcct ttagagacgg catcagcaat ttgtgtcggc gagccctggt gcatatgcaa
      661 agaaacgcgg ggataacgct caataaagcc tttgatgacg tttggtaacg cgtagcgtgc
      721 ctgggtatgc gtggtggcga tatacagcga gcctttatcc ggccaggtgt gctctccggc
      781 gaccgatttt atggcatcaa ctttcgacag gacttcgcga gcgatacgaa ttatttcttg
      841 ccctgctggc gttacctgcg tcaggtgctt gccgcttcgg gaaaaaattt gaatgcctag
      901 ctcgtcttcc agcattctga cttgtttact gatcccgggt tgtgatgtgt aaagtccttc
      961 cgctgttgat gagacattca ggttatgatt gaccacctca acaatatagc gaagttgttg
     1021 taatttcatg ttaaaccatc cacttagacg ccatcagaac atcgt
//
LOCUS       DAZFLD010000544         1064 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.547, whole genome shotgun sequence.
ACCESSION   DAZFLD010000544 DAZFLD010000000
VERSION     DAZFLD010000544.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1064)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1064)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1064
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.547"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..652)
                     /locus_tag="ACHKBL_005200"
     CDS             complement(<1..652)
                     /locus_tag="ACHKBL_005200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001615471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein"
                     /protein_id="HFO5821901.1"
                     /translation="MNKVIAVSALAMAGMFSTQALADESKTGFYVTGKAGASVMSLAD
                     QRFLSGDGEETSKYKGGDGHDTVFSGGIAAGYDFYPQFSIPVRTELEFYARGKADSKY
                     NVDKDSWSGGYWRDDLKNEVSVNTLMLNAYYDFRNDSAFTPWVSAGIGYARIHQKTTG
                     ISTWDYGYGSSGRESLSRSGSADNFAWSLGAGVRYDVTPDIALDLSYRYLDAGDSSV"
     gene            876..1061
                     /locus_tag="ACHKBL_005201"
                     /pseudo
     CDS             876..1061
                     /locus_tag="ACHKBL_005201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708738.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
BASE COUNT          307 a          269 c          231 g          257 t
ORIGIN      
        1 tcacactgct gtcacctgca tcaagatagc gatagctgag gtccagagcg atatccgggg
       61 ttacgtcata gcgaacaccc gcgccaaggc tccatgcgaa gttgtcagca gagcctgaac
      121 gtgacaacga ttcgcgacca ctgcttccgt acccataatc ccaggtactg ataccggttg
      181 ttttctggtg aattctggcg tagccaatcc ctgcagatac ccatggtgtg aatgcactgt
      241 cattccggaa gtcatagtac gcattcagca tcagtgtgtt gactgacacc tcattcttca
      301 ggtcatcacg ccagtaaccg cctgaccagc tgtctttatc tacgttatac ttcgaatcag
      361 cttttccacg agcgtaaaac tccagttccg tacgaaccgg aatactgaac tgcgggtaaa
      421 aatcataacc ggccgcgata ccgccactga ataccgtatc atggccatca ccgcctttat
      481 attttgatgt ttcctctcca tcacccgaca ggaaacgctg gtctgcaagt gacataacag
      541 aagcacctgc tttaccggtc acataaaagc ctgttttgct ctcatcagcc agagcctggg
      601 tcgaaaacat gcctgccatg gcaagcgctg aaaccgcaat aaccttattc atttcaatca
      661 tctccgctat cactttcccc gaacgataaa tggggttgct cattctaaaa ccatgcaaaa
      721 gacagggtaa aaggaatgtt ttcgatcgaa tcgatcgata aaatcgatcg tgcccgataa
      781 agttttctta tcgtccccat aaaaatggta agggctggtt taagaggtaa tcgcagcggc
      841 atggaagtat taagaccaaa acagcacgag ataaagcggc tgaacgtcag atgagccaca
      901 tggaacgtaa ttcattaagg gcggcttata tccataaaac agaacacctg gaatagcgtc
      961 ggttgatgct acagtggtgg ggggattttc tggatacaaa ctcgaaagaa aataccagcc
     1021 cctttgagta tgcgaagatt aacaatccat taaaatatta agca
//
LOCUS       DAZFLD010000545         1063 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.548, whole genome shotgun sequence.
ACCESSION   DAZFLD010000545 DAZFLD010000000
VERSION     DAZFLD010000545.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1063)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1063)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1063
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.548"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>1063)
                     /locus_tag="ACHKBL_005202"
     CDS             complement(<1..>1063)
                     /locus_tag="ACHKBL_005202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein VasK"
                     /protein_id="HFO5821902.1"
                     /translation="ILTFCAILVATLFLLGWLTEKAFDFAGKSGLYFQWGQKKAESIT
                     PVTNTIPGEEDDDEPVFSEDAVSEHLRFRYGRRWQRKVRLLLVMGDPDVVRKAAPGLC
                     HDLWQEGDGYVLIYGGDAQSLPDEIFLSKLKRLRSGQPVDGIIQVMSISALLTDGERD
                     ALLRYRQKADHQLGWQAPVWLWLTDNATGARQDAKAAATGVIFGPEGTVNGADEAFST
                     LALHLQKAGMAQILNEPADFGLLQLSLRLRQELKASLAVLLSGLMQGTAAWRLRGVMF
                     SPELTVAGAVPNTCPDTPAWKAIIDDCDAVSGRKLGFNWLKMLRLILLSLIVLWGAGT
                     LLSLLVNRTQIYQAQETAGQ"
BASE COUNT          241 a          326 c          277 g          219 t
ORIGIN      
        1 cctgtcccgc cgtttcctgc gcctgataaa tctgagtacg gttaacgagc agagacagca
       61 gtgtaccggc cccccacagg acaatcaggg aaagcagaat caatcgcagc atcttcagcc
      121 agttaaagcc cagcttcctg ccgctgacgg catcacagtc atcaataata gctttccagg
      181 caggtgtgtc ggggcatgtg tttggtaccg ctccggcaac ggtcagttca ggactgaaca
      241 tgacaccgcg taaacgccag gcagcagtcc cctgcatcag tccactgagc aaaacagcca
      301 ggctggcttt cagctcctgc cgcagtctga gtgacaactg aaggagaccg aaatcagcag
      361 gttcattcag tatctgcgcc attcctgctt tttgcaggtg taacgccagt gtggaaaacg
      421 cttcgtccgc accgttcacg gtaccttccg gtccgaagat gacccctgtt gcggccgctt
      481 tcgcgtcctg ccgggcaccg gtagcgttgt cagtcagcca cagccagact ggtgcctgcc
      541 agcccaactg atggtcggct ttctgacggt aacgcaggag ggcatcccgt tcgccatccg
      601 tcaggagggc tgaaatactc atcacctgaa tgatgccatc aaccggttga ccggatcgca
      661 gacgcttcag ttttgaaagg aagatctcgt ccgggagtga ctgcgcgtct ccgccataaa
      721 tcagcacgta gccgtcgcct tcctgccaga ggtcatggca aaggcccggg gctgctttcc
      781 ggaccacatc cggatccccc atcaccagca ggagtcgcac cttacgctgc cagcgacgcc
      841 catagcgaaa gcgcaaatgt tctgaaacag catcttcgct aaaaaccggc tcatcgtcgt
      901 cttcttcacc gggaatggtg ttcgtcaccg gagtgatact ctcagccttt ttctgccccc
      961 actggaaata cagcccggat ttaccggcga agtcgaaagc cttctccgtc agccagccga
     1021 gtaaaaacag cgtggcaacc agtatggcgc agaatgtgag tat
//
LOCUS       DAZFLD010000546         1063 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.549, whole genome shotgun sequence.
ACCESSION   DAZFLD010000546 DAZFLD010000000
VERSION     DAZFLD010000546.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1063)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1063)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1063
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.549"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>1063)
                     /locus_tag="ACHKBL_005203"
     CDS             complement(<1..>1063)
                     /locus_tag="ACHKBL_005203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein VasK"
                     /protein_id="HFO5821903.1"
                     /translation="ILTFCAILVATLFLLGWLTEKAFDFAGKSGLYFQWGQKKAESIT
                     PVTNTIPGEEDDDEPVFSEDAVSEHLRFRYGRRWQHKVRLLLVMGDPDVVRKAAPGLC
                     HDLWQEGDGYVLIYGGDAQSLPDEIFLSKLKRLRSGQPVDGIIQVMSISALPTDGERD
                     ALLRYRQKADHQLGWQAPVWLWLTDNATGARQDAKAAATGVIFGPEGTVKGADEAFST
                     LALHLQKAGMAQILDEPADFGLLQLSLRLRQELKASLAVLLSGLMQGTAAWRLRGVMF
                     SPELTVAGAVPNTCPDSPAWKAVIDDCGAASGRKLGFSWLKMLRLILLSLIVLWGTGT
                     LLSLLVNRTQIYQAQETAGQ"
BASE COUNT          232 a          330 c          283 g          218 t
ORIGIN      
        1 cctgtcccgc cgtttcctgc gcctgataaa tctgagtacg gttaacgagc agagacagca
       61 gtgtaccggt cccccacagg acaatcaggg aaagcagaat caatcgcagc atcttcagcc
      121 agctgaagcc cagttttctg ccgctggcgg caccacagtc atcaatgacg gctttccagg
      181 ccggtgagtc ggggcatgtg tttggtaccg ctccggcaac ggtcagttca ggactgaaca
      241 tgacaccgcg taaacgccag gcagcagtcc cctgcatcag tccactgagc aaaacagcca
      301 ggctggcttt cagctcctgc cgcagtctga gtgacaactg aaggagaccg aaatcagcag
      361 gctcatccag tatctgcgcc attcccgctt tttgcaggtg taacgccagt gtggaaaacg
      421 cttcgtccgc acctttcaca gttccttccg gtccgaagat gacccctgtt gcggccgctt
      481 tcgcgtcctg ccgggcaccg gtagcgttgt cagtcagcca cagccagact ggtgcctgcc
      541 agcccaactg atggtcggct ttctggcggt aacgcaggag ggcatcccgt tcgccatccg
      601 tcgggagggc tgaaatgctc atcacctgaa tgatgccatc aaccggttgc ccggatcgca
      661 gacgcttcag ttttgaaagg aagatctcgt ccgggagtga ctgcgcgtct ccgccataaa
      721 tcagcacgta gccgtcgcct tcctgccaga ggtcatggca aaggcccggg gctgctttcc
      781 ggaccacatc cggatccccc atcaccagca ggagtcgcac cttatgctgc cagcgacgcc
      841 catagcgaaa gcgcaaatgt tctgaaacag catcttcgct gaaaaccggc tcatcgtcgt
      901 cttcttcacc gggaatggtg ttcgtcaccg gagtgatact ctcagccttt ttctgccccc
      961 actggaaata cagcccggat ttaccggcga agtcgaaagc cttctccgtc agccagccga
     1021 gtaaaaacag cgtggcaacc agtatggcgc agaatgtgag tat
//
LOCUS       DAZFLD010000547         1060 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.550, whole genome shotgun sequence.
ACCESSION   DAZFLD010000547 DAZFLD010000000
VERSION     DAZFLD010000547.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1060)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1060)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1060
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.550"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            81..>1060
                     /locus_tag="ACHKBL_005204"
     rRNA            81..>1060
                     /locus_tag="ACHKBL_005204"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          283 a          231 c          333 g          213 t
ORIGIN      
        1 aagacgaaaa atgaatacca agtctcaaga gtgaacacgt aattcattac gaagtttaat
       61 tctttgagca tcaaactttt aaattgaaga gtttgatcat ggctcagatt gaacgctggc
      121 ggcaggccta acacatgcaa gtcgaacggt aacaggaaac agcttgctgt ttcgctgacg
      181 agtggcggac gggtgagtaa tgtctgggaa actgcctgat ggagggggat aactactgga
      241 aacggtagct aataccgcat aacgtcgcaa gaccaaagag ggggaccttc gggcctcttg
      301 ccatcggatg tgcccagatg ggattagcta gtaggtgggg taacggctca cctaggcgac
      361 gatccctagc tggtctgaga ggatgaccag ccacactgga actgagacac ggtccagact
      421 cctacgggag gcagcagtgg ggaatattgc acaatgggcg caagcctgat gcagccatgc
      481 cgcgtgtatg aagaaggcct tcgggttgta aagtactttc agcggggagg aagggagtaa
      541 agttaatacc tttgctcatt gacgttaccc gcagaagaag caccggctaa ctccgtgcca
      601 gcagccgcgg taatacggag ggtgcaagcg ttaatcggaa ttactgggcg taaagcgcac
      661 gcaggcggtt tgttaagtca gatgtgaaat ccccgggctc aacctgggaa ctgcatctga
      721 tactggcaag cttgagtctc gtagaggggg gtagaattcc aggtgtagcg gtgaaatgcg
      781 tagagatctg gaggaatacc ggtggcgaag gcggccccct ggacgaagac tgacgctcag
      841 gtgcgaaagc gtggggagca aacaggatta gataccctgg tagtccacgc cgtaaacgat
      901 gtcgacttgg aggttgtgcc cttgaggcgt ggcttccgga gctaacgcgt taagtcgacc
      961 gcctggggag tacggccgca aggttaaaac tcaaatgaat tgacgggggc ccgcacaagc
     1021 ggtggagcat gtggtttaat tcgatgcaac gcgaagaacc
//
LOCUS       DAZFLD010000548         1060 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.551, whole genome shotgun sequence.
ACCESSION   DAZFLD010000548 DAZFLD010000000
VERSION     DAZFLD010000548.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1060)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1060)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1060
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.551"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..576
                     /locus_tag="ACHKBL_005205"
     CDS             <1..576
                     /locus_tag="ACHKBL_005205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001379928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO5821904.1"
                     /translation="AWLKEKGAFDQPYWMMKASLLAEFNKVITDVGPGKLNLGGCAIE
                     VMGTYNAAIVRVTIGEYGGDGFLNGTVCTCTVYGDTQRFHWRVDYSTKNKPTTASLTV
                     NGWERDEVTGRLRQWGSIEVSEDDGKLMTQHSIRFPVAFPVAALNAQVSAVGDPHAIK
                     MYTLSNPSLVSVTLITVRGCYGKFSWEAIGY"
     gene            586..810
                     /locus_tag="ACHKBL_005206"
     CDS             586..810
                     /locus_tag="ACHKBL_005206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001367204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO5821905.1"
                     /translation="MEQYVFSPSENMFYPLSLRPVYEAAGRWPEDGIVVDYVVYKVFA
                     ADAAPAGMKRGVGAEKMPVWVPVSEEGTET"
     gene            807..>1060
                     /locus_tag="ACHKBL_005207"
     CDS             807..>1060
                     /locus_tag="ACHKBL_005207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000972077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO5821906.1"
                     /translation="MMHIRNFSYYTPAEPDVAGAMYLKSEDGQDWYECQALFSPETLK
                     VVYDSRGVITGYGKDTALLWPVNQSVAEVPDTPESRKIDL"
BASE COUNT          251 a          221 c          340 g          248 t
ORIGIN      
        1 gcctggctga aagaaaaggg ggcatttgac cagccttact ggatgatgaa ggcatcactg
       61 cttgcggaat ttaataaggt catcacggat gtcggaccgg gaaaactcaa tctgggaggc
      121 tgcgccattg aggtgatggg gacgtataac gcagccatag tccgggtcac catcggcgaa
      181 tacggtggcg atggttttct gaacggcacg gtctgtacct gtacagttta cggagacaca
      241 caacgttttc actggcgggt ggattacagc acaaaaaaca agcccacaac agcaagcctg
      301 acagtaaatg gctgggagcg tgatgaagta accggtcgtt tgcggcaatg gggcagtatt
      361 gaggtaagtg aggatgatgg taagcttatg acacagcact ctatccgttt tcctgtcgct
      421 tttccggttg ctgctctgaa tgctcaggtg tctgcagtgg gagaccccca cgccattaag
      481 atgtataccc tgtcgaatcc gtcattagtg tctgtaactc tgataacagt caggggatgc
      541 tacggcaagt tttcatggga agcgatcgga tactgagggc attgtatgga acagtatgtt
      601 ttttcaccgt cagagaatat gttctatccc ctgtccctgc ggccggttta tgaggcagca
      661 gggcgctggc cggaagacgg tattgtggtg gattatgtgg tgtataaagt ctttgcagcg
      721 gatgctgcac cggcaggtat gaagcggggc gtcggtgcgg aaaaaatgcc ggtctgggtg
      781 ccggtatcgg aggagggcac ggaaacatga tgcatatcag aaatttttcg tattacaccc
      841 cggcggagcc ggatgttgca ggcgcgatgt acctgaagtc tgaggatggt caggactggt
      901 atgagtgtca ggcgttgttt tcgccggaga cgctgaaggt ggtgtatgac agccggggtg
      961 tgattacggg atacggtaag gacactgcgt tactgtggcc ggtgaaccag agtgtggcag
     1021 aagtgccgga taccccggag agccggaaaa ttgacctgag
//
LOCUS       DAZFLD010000549         1059 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.552, whole genome shotgun sequence.
ACCESSION   DAZFLD010000549 DAZFLD010000000
VERSION     DAZFLD010000549.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1059
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.552"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..841
                     /locus_tag="ACHKBL_005208"
     CDS             <1..841
                     /locus_tag="ACHKBL_005208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HFO5821907.1"
                     /translation="NALKLEQTKLTLVTMTMQDHATLKEIKDLAKNITQQYGTPDLCI
                     EAMRKKNLKTMIFISQLKAKTNFQVVGLTMKCYSPLCKSASRMDQNLRAPVPMTWAFM
                     MREAGFPAVRPKYELPLSKDVLDEVREEMRSLGSYIALNLEGSSQERTFSLSIAENLI
                     EKIQSETDIPIVIVHGPKGEEKARVLVDCYNNVFRLSLSPSIKRSAAIIKDAYIAITP
                     DTSILHMASAYNTPVVAIYADYKTRWPAMADVSESVVVGQKIDNISLDEFAKALKSVL
                     LRI"
BASE COUNT          342 a          197 c          236 g          284 t
ORIGIN      
        1 taatgcgctc aaacttgaac agactaaatt aacattagtt acaatgacta tgcaggatca
       61 cgcaacatta aaagaaataa aagatctagc gaagaacata acacagcaat acggtacgcc
      121 ggatctttgt atagaggcga tgcgtaaaaa gaacctgaaa acgatgatat ttatcagtca
      181 gttgaaagca aaaacgaatt ttcaggttgt tggtttaacc atgaagtgct attccccttt
      241 gtgtaagagc gcgtcccgga tggatcagaa tctccgggct cctgtaccca tgacatgggc
      301 atttatgatg cgtgaggcgg gttttccagc agtcaggcca aaatatgaat tgccactaag
      361 taaggatgta ctcgatgagg tgcgcgagga aatgcgatcg ttaggatctt acattgcgct
      421 taatttagaa ggtagctcgc aggaacgtac attttcatta tcgattgcag aaaatctaat
      481 agaaaaaatt caaagtgaaa cagatatacc aatagtgatc gttcatggac caaaaggcga
      541 agaaaaagcc agggtgttag tggattgtta taataatgtc ttccgtttat ccttatcacc
      601 ctcgattaaa cgttcggcag caatcataaa agatgcttat atcgcaataa ctcctgacac
      661 ctcaatatta catatggcaa gtgcctataa tacccccgtt gttgcaattt atgctgatta
      721 caaaacgcga tggcccgcaa tggccgatgt ctcggagtca gtcgtcgttg ggcaaaaaat
      781 tgacaatata agtctggatg aattcgcaaa ggcattaaaa agtgttttgt tgagaatatg
      841 aggcagtact tatgcatagt agtgccgatc tgttatgaac ggtggtgtca tcaatacttt
      901 gtggctggtc aatcattaca gaaagccagc ctttggatca aagctgtact caccggatac
      961 aaccatagtc acaccgaacg cgaatctggc ggtatcgcat ttttatttat tcattttctg
     1021 tgagagaagg gcaagttctg tagagagaat gtaaaaaag
//
LOCUS       DAZFLD010000550         1046 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.553, whole genome shotgun sequence.
ACCESSION   DAZFLD010000550 DAZFLD010000000
VERSION     DAZFLD010000550.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1046)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1046)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1046
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.553"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            177..>1046
                     /locus_tag="ACHKBL_005209"
     CDS             177..>1046
                     /locus_tag="ACHKBL_005209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
                     /protein_id="HFO5821908.1"
                     /translation="MSEWCKNRFEITGKSVCLDVLTQWIEGRETPRYRHAIQQSIQLF
                     LAGCAGLVKPTRTTQYPPYPALVRHGTGLSSPANQAFEQWLGLLVKNAVLDGETIKII
                     ERLYHQSCIGAVRWGNLPDNAREIITTLMSRQYSDWFGLAGLSEHIDAEACWSWLSDY
                     PEQAQPCDMLMVIPSRLATELNGSGGLLQGISTTTSLYSRIYGVEWPSGNNVRHARDE
                     MSSLVLLIDTPWYPPSGELVGEISKVFDCEVRHWYSEPVHGIQGYNCYDGGEHTDSDL
                     QAEWPGRETLPQPR"
BASE COUNT          243 a          251 c          308 g          244 t
ORIGIN      
        1 caggcacgtg aagcatcaga gtatttactg acgcggcgaa cttcgcagga gtgcgcagca
       61 taatttttac taccggtggc ggttataacc tgaccgccat ttatcccgtg agggcattgt
      121 acctcacggg acaatgccct ctttttacat ctgtataaaa aagaggagcg taaacaatgt
      181 ctgaatggtg caaaaaccgt tttgaaatta ccggtaaatc agtctgtctt gatgtactta
      241 cacagtggat tgaagggcgt gagaccccgc gctaccgtca tgccatacag cagagtatac
      301 agctgtttct ggccggttgt gcagggctgg taaaacccac acggacgacg cagtatccgc
      361 catatccggc gctggttcgt cacggaacgg ggttgtcatc tccggctaac caggcgtttg
      421 agcagtggct ggggttgctg gtaaaaaatg ccgttctgga tggtgaaacg ataaagatca
      481 ttgagcgtct ttatcaccag tcatgtatcg gcgctgttcg ctggggtaat ctgccggata
      541 atgccaggga gatcatcacc acgctgatga gccgtcagta ttctgactgg tttggtctgg
      601 ccgggctgag tgaacacatt gatgcagaag cctgctggag ctggttgtcg gattacccgg
      661 aacaggcgca gccctgtgac atgctgatgg tcataccctc ccgtctggct acagagctga
      721 atggttcagg tggactgctg cagggtattt ccacaacgac cagcctttac agccggatat
      781 acggtgtgga gtggccgtca ggaaataatg ttcgccatgc gcgtgatgag atgagcagtc
      841 tggtgctgct gatcgacacg ccctggtatc cgccttcagg cgaactggtt ggtgagatct
      901 cgaaggtgtt tgactgcgag gtccgacact ggtacagcga gccggtacac ggtatacagg
      961 gttacaactg ttacgatggc ggagagcata cggacagtga tctgcaggcc gaatggccgg
     1021 gcagggaaac acttccacag ccccga
//
LOCUS       DAZFLD010000551         1043 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.554, whole genome shotgun sequence.
ACCESSION   DAZFLD010000551 DAZFLD010000000
VERSION     DAZFLD010000551.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1043)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1043
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.554"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>1043
                     /locus_tag="ACHKBL_005210"
     CDS             <1..>1043
                     /locus_tag="ACHKBL_005210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000033404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFO5821909.1"
                     /translation="FDLNKSFGINKFIIIVPTLSIKAGTVNFLKSDAIKEHFRDDYER
                     ELKTYVVESQKSSGKNTKSYMPQAIHDFVEASNFNKKYIHILVINSGMINSKSLTDTY
                     DVGLLDNQFDTPFSALSAIKPFIIIDEPHKFPTGKKTWEHIQKFNAQYIIRYGATFSE
                     GYKNLVYRLTAVDAFNEDLVKGIDAYIEDIVGDGNANLKLVKSDGKEATFELNENNNK
                     KIFKLAKGESLSKTHSAIHDLTLDALNKNTAVLSNGIELKIGSSINPYSYDQTLADNM
                     MRKAVKEHFKLEKEYLTQRPRIKPLTLFFIDDIEGYRDGNDIAGSLKTKFEEYVLAEA
                     NELLKTEKDEFYR"
BASE COUNT          369 a          175 c          188 g          311 t
ORIGIN      
        1 ttttgacctg aacaaatcat ttggtattaa caaatttatc atcattgttc ctaccctatc
       61 gatcaaagcc ggaaccgtta actttttaaa aagcgatgct ataaaagaac actttcgaga
      121 tgattacgag cgtgaactaa agacttacgt tgttgaaagt cagaaaagtt cgggtaaaaa
      181 tacaaaatcg tatatgcctc aagctatcca tgattttgtt gaagcaagta atttcaataa
      241 gaaatatata cacattcttg tgatcaactc agggatgatt aactctaagt ctttaactga
      301 cacttatgat gttggcttgc ttgataatca gtttgatacc ccattttctg cacttagtgc
      361 gataaaacca ttcattatca ttgatgagcc tcataaattt cctactggta aaaaaacatg
      421 ggaacatatt caaaaattta atgcccaata tatcatccgc tatggcgcaa cctttagtga
      481 aggctataag aacttagttt atcgtcttac agccgtagat gccttcaatg aagatcttgt
      541 taaaggtatt gatgcataca ttgaggatat tgttggtgat ggtaatgcca acctcaagtt
      601 ggtcaaatct gacggaaaag aagctacatt tgaactgaac gaaaataata ataaaaaaat
      661 ttttaagtta gccaaaggtg aatcactatc taagacgcat agtgcgatcc atgatttaac
      721 tcttgatgct ttaaataaga atacagcagt gttgagtaat ggtattgagc taaaaattgg
      781 tagctcgata aacccctatt cttacgatca gacacttgct gataacatga tgcgtaaagc
      841 tgtcaaagaa cactttaagt tagaaaaaga gtatctgaca caaaggccac gcataaaacc
      901 tcttactctt ttcttcattg atgacattga aggctatcgt gatggtaatg atattgcagg
      961 aagcctgaaa actaagttcg aagaatacgt tttggcagaa gcaaatgaac tgttaaaaac
     1021 agagaaagat gagttttaca gaa
//
LOCUS       DAZFLD010000552         1038 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.555, whole genome shotgun sequence.
ACCESSION   DAZFLD010000552 DAZFLD010000000
VERSION     DAZFLD010000552.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1038)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1038)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1038
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.555"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..956)
                     /gene="subA"
                     /locus_tag="ACHKBL_005211"
     CDS             complement(<1..956)
                     /gene="subA"
                     /locus_tag="ACHKBL_005211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001380856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="subtilase AB5 cytotoxin subunit A"
                     /protein_id="HFO5821910.1"
                     /translation="MLKILWPHILFLLFISVSVRAEKPWYFDAIGLTETTMSLTDKNT
                     PVVVSVVDSGVAFVGGLSDSEFAKFSFTQDGSPFPVKESEALYIHGTAMASLIASRHG
                     VYGVYPHALISSRRVIPDGVQDSWIRATESIMSNVFLAPGEEKIINISGGQKGISSAS
                     VWSELLSRMGRNNERLIVAAVGNDGADIRKMSAQQRIWPAAYHPVSSVNKKQDPVIRV
                     AALAQYRKGETPVLHGGGVTGSRFGNGWVDIAAPGQNITFLRPDGKTGIGSGTSEATA
                     IVSGVLAAMVSCNPRATATELKRTLLESADKYPSLADKVTEG"
BASE COUNT          273 a          275 c          212 g          278 t
ORIGIN      
        1 ctcccttccg tcaccttatc tgcaagtgag gggtatttgt ctgcagactc cagcagtgta
       61 cgcttcagtt ctgtcgctgt tgcacgggga ttacatgaga ccatcgctgc cagaacgccg
      121 gaaactatag ctgttgcttc cgaggttcca ctaccgatcc ccgtttttcc atctggtctg
      181 aggaatgtaa tattctgtcc cggtgcggca atatcaaccc agccgtttcc gaaccgactt
      241 ccggtgaccc ctccaccatg caatacaggt gtttctcctt tccggtactg tgccagagct
      301 gccactctta tcacaggatc ctgcttttta ttcacactgc tgaccggatg gtaagccgca
      361 ggccatattc tctgctgagc actcattttt cgtatatctg cgccatcatt acccactgcc
      421 gcaacaatta atcgctcatt attccggccc atacgggaaa gcagttcgga ccagaccgat
      481 gcggaagata tccccttctg gcctcccgat atattaatga ttttctcttc tcctggtgca
      541 agaaaaacat ttgacataat gctttcagtt gctctaatcc atgagtcctg tacaccgtca
      601 ggaataaccc ttctgctgga tatcagtgca tgaggataaa caccataaac cccatgacgt
      661 gaggcaatga gtgaagccat ggcagtacca tgaatatata aagcttcaga ctcttttacc
      721 gggaatggcg aaccatcctg agtaaaactg aatttggcaa attcgctgtc gctgaggcct
      781 cctacaaatg ctactccgga atcaacaaca gatacgacaa caggggtatt cttgtctgtg
      841 agcgacatgg ttgtttctgt caggccaatt gcatcaaaat accagggttt ttctgccctg
      901 acagacacag atataaaaag caggaacaga atatgcggcc ataaaatctt aagcacaata
      961 tcctccagtc agtacaaata aacagtattg caggtcaata tcttacagga acatcacatc
     1021 tgaaatatcc tgttttcg
//
LOCUS       DAZFLD010000553         1037 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.556, whole genome shotgun sequence.
ACCESSION   DAZFLD010000553 DAZFLD010000000
VERSION     DAZFLD010000553.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1037)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1037)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1037
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.556"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..782)
                     /locus_tag="ACHKBL_005212"
     CDS             complement(<1..782)
                     /locus_tag="ACHKBL_005212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000991381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFO5821911.1"
                     /translation="MNAIIPHKRRDGRSSFEDLIAYTSVRDDMQENELTEDSEIKSDV
                     PHRNRFRRLVDYATRLRNEKFVSLIDVMKDGSQWVNFYGVTCFHNCNSLETAAEEMQY
                     KADKAVFSRNDTDPVFHYILSWPAHESPRPEQLFDCVRHTLKSLELSKHQYVAAVHTD
                     TDNLHVHVAVNRVHPVSGHINRLSWSQERLSRACRELELKHGFAPDNGCFVHAPGNRI
                     VRKTALVRERRNAWRRGKKQTFREYIAQMSIAGLREEPAQDW"
     gene            complement(794..>1037)
                     /gene="mobA"
                     /locus_tag="ACHKBL_005213"
     CDS             complement(794..>1037)
                     /gene="mobA"
                     /locus_tag="ACHKBL_005213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="plasmid mobilization protein MobA"
                     /protein_id="HFO5821912.1"
                     /translation="VREKAESSGQTVSTFIRSSSLDKVVNCRTDDRMIDEIMRLGRLQ
                     KHLFVEGKRTGDKEYAEVLVAITEYVNALRRDLMGR"
BASE COUNT          262 a          270 c          238 g          267 t
ORIGIN      
        1 aaccagtcct gtgcaggctc ttcacgcagc ccggcaatcg acatctgagc aatatattcc
       61 ctgaacgtct gctttttccc gcgtcgccag gcattacggc gctcacgtac cagcgctgtt
      121 ttgcgcacaa tacggttgcc gggtgcatgc acaaagcagc cattatcagg cgcgaatccg
      181 tgcttaagtt caagttcacg acatgcacgg ctgagccttt cctgactcca ggaaagacga
      241 ttaatatgac cactaacagg atgaacacga ttcactgcga catgtacatg caggttatcc
      301 gtgtccgtat gaacggcagc cacgtactga tgttttgaga gttcaaggga tttcaatgtg
      361 tgacgcacac agtcaaaaag ctgctcagga cgcgggcttt catgcgcagg ccaggagaga
      421 atgtagtgga aaacggggtc cgtatcatta cgggaaaaaa cagctttgtc cgccttgtac
      481 tgcatctctt cagcagctgt ttccagtgaa ttgcagttat ggaaacaggt aacgccatag
      541 aaatttaccc actgactgcc gtctttcatc acgtcaatca gggagacaaa tttttcgtta
      601 cgcaggcgtg tagcatagtc gacaaggcgt ctgaaacggt tgcggtgagg gacgtcactc
      661 ttgatttcgc tgtcttctgt gagctcattt tcctgcatat catcccgcac agatgtgtac
      721 gcaatcaggt cttcaaacga agacctgccg tcacgtcttt tgtgcggaat aatggcattc
      781 atcagcagtt atctcagcgt cccattaagt ctcttcgcag agcattgaca tattcagtaa
      841 tggcaacaag aacctcggca tactccttat caccagtacg ttttccttcc acaaacagat
      901 gcttctgaag gcggccaaga cgcataatct catcaatcat acggtcgtca gtacggcagt
      961 taaccacctt atccagtgaa gagcttcgta taaatgttga aaccgtctgt ccacttgact
     1021 ccgctttttc tctgacg
//
LOCUS       DAZFLD010000554         1036 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.557, whole genome shotgun sequence.
ACCESSION   DAZFLD010000554 DAZFLD010000000
VERSION     DAZFLD010000554.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1036
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.557"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            158..463
                     /locus_tag="ACHKBL_005214"
     CDS             158..463
                     /locus_tag="ACHKBL_005214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001604127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5821913.1"
                     /translation="MNPLFNDIQMRLFYLNHAPYSWHWNVRFRPQEAVYIGNDACHIT
                     ITCNQSGFHLTRDGQRLFTERYIRTLSELLAVLKRRWDVTPAIIRAVEYLSRVPVSH"
     gene            518..>1036
                     /locus_tag="ACHKBL_005215"
     CDS             518..>1036
                     /locus_tag="ACHKBL_005215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000200223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ImpA family N-terminal
                     domain-containing protein"
                     /protein_id="HFO5821914.1"
                     /translation="MTSEVKLKTGNDPRSLPDYAALRDEISKLTHPARPDVDWRYVET
                     LCLRLYEHNGVELQTASWYTIARMHTTGLSGLNEGLALIVALTRHHWPVMWPLNTHAR
                     LEIITGLFSRLQKTLRAMPPDERDNLPLLYQTETCLKTLSDILTRHELKQSSKVALPE
                     AMVKGYITRLENQ"
BASE COUNT          262 a          247 c          273 g          254 t
ORIGIN      
        1 ggactggttc gttacggaac aacttttatg ccggagggca gggtgttact gcgatatctg
       61 aagcaacagc agtttgtgga taatgtgatg actttcaggg ggtgatttat ggttcccctt
      121 atccgtgtgc ttaacagaca taaacgaggg ctgaatgatg aacccgttat ttaatgacat
      181 ccagatgcgc cttttttatc tgaatcatgc accgtattcc tggcactgga atgtcagatt
      241 caggccacag gaagccgtat atattggtaa tgatgcctgc catattacca ttacctgtaa
      301 tcagtccggt tttcatttaa ccagggacgg gcagcgactg ttcactgaac gttatatcag
      361 aacactcagt gagttattgg cggttctgaa gcggcggtgg gatgtgacac cggcaatcat
      421 ccgtgcggtg gaataccttt cccgggtacc ggtatcacac tgacgctctt tccgggaata
      481 ttaattatga tttgatacag caacactgaa tctgccgatg acttctgaag taaaactgaa
      541 aacaggtaat gacccccggt cattaccgga ttatgctgcc ctgcgcgatg aaatcagcaa
      601 gctgactcac ccggcacgtc cggatgtgga ctggcgttat gtggagacgc tctgcctgcg
      661 tctgtatgaa cacaatggcg tggaattaca gacggcatcc tggtacacca tcgcccggat
      721 gcatacaaca gggttaagtg gcctgaatga agggctggct ctgattgtgg cgctgacccg
      781 gcatcactgg ccagtgatgt ggccgctgaa tacccacgcc cggctggaaa ttattaccgg
      841 tctgttcagt cgtctgcaaa aaacgctccg ggccatgcca ccggatgagc gtgacaacct
      901 gcccctgctg tatcagacag aaacatgcct gaaaacgctg agtgacatac tgacccggca
      961 tgaacttaaa cagagcagta aagtggcttt accggaagcc atggtgaagg gatatatcac
     1021 ccgtctggag aatcag
//
LOCUS       DAZFLD010000555         1027 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.558, whole genome shotgun sequence.
ACCESSION   DAZFLD010000555 DAZFLD010000000
VERSION     DAZFLD010000555.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1027
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.558"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..908)
                     /locus_tag="ACHKBL_005216"
     CDS             complement(<1..908)
                     /locus_tag="ACHKBL_005216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309259.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5821915.1"
                     /translation="MPYIDITTMRGMMPGVIASMLPDHSAVLAENCHFRYGVITPEHQ
                     MSEAEKTFTIKPKTIFHYRDDFWFAWPDVVDVIRSPVAQDAHGRIYYTDGRFPKVTDA
                     TIATKGDGNHPTSSYRLGIPAPTTAPVCTVQQGGDVSDDNPNDDETRFYTETFVSDYG
                     EEGPPGPASLEVTLRTPGTAVQLTLSPVPLQNASIKRRRIYRSASGGGEADFLLVAEL
                     DASVLSYTDKIPGKNLGPSLATWDYLPPPENMTGLCLMANGIAAGFAGNEVMFSEAYL
                     PYAWPEVNRHTTAEDIVAICPLGTSLV"
BASE COUNT          262 a          273 c          248 g          244 t
ORIGIN      
        1 accagtgacg ttcccagcgg acagatagcc acaatatctt ctgccgtcgt gtgacgattc
       61 acttccggcc atgcatacgg cagatacgct tccgaaaaca tcacttcatt accggcaaac
      121 ccggcggcaa taccattagc catcaggcaa aggcctgtca tattctctgg cggcggcagg
      181 taatcccatg tcgccagaga aggtccaagg tttttccccg gtattttgtc cgtgtaactg
      241 agcacggatg catccagttc agccacaagt aaaaaatccg cctcccctcc acctgatgca
      301 gagcgataaa tccggcggcg tttaatactg gcattctgca atggcaccgg agacagcgtc
      361 agctgtaccg cagtccccgg agtacggagt gttacctcca gagacgccgg acctggcgga
      421 ccttcttcac cataatctga gacaaaggtt tccgtataaa accgggtttc gtcatcattc
      481 gggttatcgt cagaaacatc accgccctgc tgaacagtac agacaggtgc tgttgtcggc
      541 gcggggatcc ccagacgata cgatgatgtc gggtgattcc cgtccccttt tgtggcaatg
      601 gtcgcatccg tcactttagg aaaacgcccg tcagtgtagt aaatacgccc gtgggcgtcc
      661 tgagcgaccg gactgcggat cacatccacc acatccggcc acgcaaacca gaaatcatca
      721 cggtaatgaa aaatggtttt cggcttaatt gtgaatgttt tctcagcctc tgacatctgg
      781 tgttcaggcg tgatcactcc atagcgaaaa tgacagtttt ctgccagtac agcagaatga
      841 tctggcagca tagatgcaat aacgcctggc atcatcccgc gcatagttgt tatatcgata
      901 taaggcatgg ttgtctggta tccgaaaatt tgaatggcag gtgagtcact gaattgaagt
      961 tttaaaggcg gcatacaatt gcgcctgtgc agaatacaga tttccagaaa tctgaaaaat
     1021 tactctt
//
LOCUS       DAZFLD010000556         1026 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.559, whole genome shotgun sequence.
ACCESSION   DAZFLD010000556 DAZFLD010000000
VERSION     DAZFLD010000556.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1026
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.559"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            42..>1026
                     /locus_tag="ACHKBL_005217"
     CDS             42..>1026
                     /locus_tag="ACHKBL_005217"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HFO5821916.1"
                     /translation="MEKVYITGAKRTPIGCFGGSLSTVSASDLGAIAISAAIKQSRIN
                     VECIDEVIVGNVLSAGQGMGPGRQAAVKAGIPWKTPALTLNMICGSGMKTIIDAAMKI
                     RSGDMDVVVTAGIENMSQAPYLIDHHARSGVRMGNQVMSDSLITDGLTDAFNHYHMGI
                     TAENIARQFNITRYEQDTFAFESQKRALKAIDNGEFEDEITPVQVHTRKGNIEVTTDE
                     YPRRDSSLEKLSVLRPAFEKNGTVTAGNSSGINDGAAALILASEKAIQTLELQPLAEI
                     RSFGQGGVDASVMGLGPVPAIDNALKKAKLDIKDIDLFEINEAFAAQAIGVL"
BASE COUNT          331 a          155 c          253 g          287 t
ORIGIN      
        1 cattaaaaat tgatggtgga ttagttattt aaggaggagg aatggaaaaa gtatacatta
       61 caggggcaaa aagaacgcct ataggatgtt ttggcggttc gttatcaaca gtgtcagctt
      121 cagatcttgg agcaattgcg atatctgcag caattaaaca gagcagaata aacgttgaat
      181 gtattgatga agttattgtc gggaatgttt tgtctgctgg acaaggaatg gggccaggta
      241 gacaagctgc tgttaaagcc ggtataccat ggaaaactcc ggctctgact ttgaatatga
      301 tttgtggtag tggtatgaaa actattatcg atgctgcaat gaaaattcgt agtggagata
      361 tggatgttgt tgttacggca ggcatagaga atatgagtca ggcaccttac ttgattgatc
      421 atcatgcaag aagtggtgta cgaatgggta atcaggtaat gagtgactct cttattacag
      481 atggtcttac tgatgctttt aatcactatc atatggggat tacagcagaa aatatagccc
      541 gacagttcaa tattacaaga tatgaacagg atacatttgc gtttgaaagt cagaagagag
      601 ccttaaaggc cattgacaat ggagaatttg aggatgaaat tactccggta caagtacata
      661 cccgaaaagg aaatattgaa gttactacag atgagtatcc tcgtagagac tcctcattag
      721 agaaactttc tgtgttacgt ccagcatttg aaaaaaatgg taccgtgact gcaggaaatt
      781 catctggaat aaacgatggt gcagcagctc ttattcttgc ttcagagaaa gcaattcaga
      841 cattggaatt acaacctttg gctgaaattc gctcatttgg acaaggaggc gttgatgcat
      901 cagtgatggg gctggggcct gttccagcaa tagataatgc attaaaaaaa gcaaaactgg
      961 atattaaaga tattgatctt tttgaaatta acgaggcgtt tgcggctcag gcaatagggg
     1021 tcttaa
//
LOCUS       DAZFLD010000557         1024 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.560, whole genome shotgun sequence.
ACCESSION   DAZFLD010000557 DAZFLD010000000
VERSION     DAZFLD010000557.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1024
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.560"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            208..972
                     /gene="nfeF"
                     /locus_tag="ACHKBL_005218"
                     /gene_synonym="yqjH"
     CDS             208..972
                     /gene="nfeF"
                     /locus_tag="ACHKBL_005218"
                     /gene_synonym="yqjH"
                     /EC_number="1.16.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent ferric chelate reductase NfeF"
                     /protein_id="HFO5821917.1"
                     /translation="MNNTPRYPQRVRNDLRFRELTVLRVERISVGFQRIVLGGEALDG
                     FTSRGFDDHSKLFFPQSDAHFVPPTVTEEGIVWPEGPRPPSRDYTPLYDELRHELAID
                     FFIHEGGVASSWAMQAQPGDKLTVAGPRGSLVVPEDYAYQLYVCDESGMPALRRRLET
                     LSKLAVKPQVSALVSVRDNACQDYLAHLDGFNIEWLAHDEQAVDARLAQMQIPADDYF
                     IWITGEGKVVKNLSRRFEAEQYDPQRVRAAAYWHAK"
BASE COUNT          245 a          257 c          273 g          249 t
ORIGIN      
        1 acgtggactc atctcacacg taggccggat aaggcgttca cgccgcatcc ggcgttgagt
       61 aatgatgcct gagaagaggc ggttcgtctt atcagactat agacatatca gttctacaaa
      121 tcgcttgcat ttatcatgat taacaatcat tatcatttgc gaaattttat ttagatatat
      181 ctgattacca tcatgaaggc gataacaatg aataacaccc cccgctaccc acagcgcgtt
      241 cgcaatgatc tgcgcttccg tgaattgact gtgctacgcg ttgagcgtat tagcgtcggt
      301 tttcagcgca ttgtccttgg cggcgaggcg ctggacggtt ttacatcgcg cggctttgac
      361 gatcacagca aactcttctt tcctcaatct gatgctcact ttgtgccgcc aacggtaacg
      421 gaagagggca tcgtctggcc ggaaggacca cgcccaccgt cgcgtgacta tacgccgctg
      481 tatgacgaac tacgccatga actggcgatt gatttcttta ttcacgaagg tggcgtcgcc
      541 agcagctggg caatgcaggc gcaaccgggc gataaactca cggtggcagg tccgcgcggt
      601 tcgctggtgg tgccagagga ttacgcgtat cagctgtatg tctgcgatga atccggaatg
      661 cccgcactgc gccgccgcct ggaaacgttg agcaaacttg ccgttaaacc gcaagttagt
      721 gcgctggtta gcgtgcggga taacgcctgt caggattatc tcgcgcacct tgatggtttt
      781 aatatcgaat ggctggcgca tgatgagcag gcggtagatg cgcgtctggc gcagatgcaa
      841 atccctgccg atgattactt catctggata accggcgaag gcaaagtcgt taagaattta
      901 agccgccgct ttgaagcgga acagtatgac ccacagcggg tacgtgcagc ggcttactgg
      961 cacgcaaagt aactgatata taaaaagaaa aaggctgacg atttctcgtc agcctttgct
     1021 atat
//
LOCUS       DAZFLD010000558         1022 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.561, whole genome shotgun sequence.
ACCESSION   DAZFLD010000558 DAZFLD010000000
VERSION     DAZFLD010000558.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1022)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1022
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.561"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            331..528
                     /locus_tag="ACHKBL_005219"
     CDS             331..528
                     /locus_tag="ACHKBL_005219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001367422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5821918.1"
                     /translation="MILSRKKIEIEVVQSGTADNPETVTKIKVSRPEDLEACGVIVEK
                     ICAIHAKSVNTTASNDHNKYN"
     gene            complement(644..784)
                     /locus_tag="ACHKBL_005220"
     CDS             complement(644..784)
                     /locus_tag="ACHKBL_005220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFO5821919.1"
                     /translation="MPQKTIIVSILCITILMIVWMIHKSPCEFRLNIMWSQFAAFLQC
                     KQ"
BASE COUNT          261 a          250 c          241 g          270 t
ORIGIN      
        1 tccagacggt caacaacatg gttaagcata aaatgctcac gatgatcgtt ttttgcggca
       61 tagcgcttga ctccttttac agggaggcgc taaccttcca cttgtcacgg taggaagtgt
      121 tagggcctcg ggttaacatt taagttgact cggggccttt ccacatccgg ccttcaggta
      181 atccttccag ccatcagccg aaaggcaccc gcgtgtaatg tactgatttc cctttgtgtg
      241 cgcaatgcct ggcgttgaag cagacaacgc tatcgcactg gtgatattct ttcgcacagg
      301 cttactctgt cctgtgtcgg ggcgatgaaa atgattcttt caagaaaaaa aattgaaatt
      361 gaggttgtgc aaagtggaac ggctgataat cctgagacgg ttacaaaaat aaaggtttca
      421 cgcccggaag atcttgaagc gtgtggtgtt atcgtggaaa aaatttgtgc tattcatgca
      481 aaatcagtta ataccactgc cagtaacgac cacaataaat acaactgaac aaaaaagccc
      541 gcatattgcg ggcaaatacg tctgtaatcc aacggagaaa gggtgccata tcgaccgaac
      601 ccggcaaccg gatagcgggg gctttcaccc ccgctgcggt ttcttactgt ttacactgca
      661 agaacgccgc aaattgtgac cacatgatgt taagcctgaa ctcacacggt gacttgtgta
      721 tcatccagac aatcatcaaa atagttatgc ataaaatgct cactatgatc gttttttgcg
      781 gcatagcgct tgactccttt tacagggagg cgctaacctt ccacttgcga ggtagatggg
      841 ttagggcctc gggttaaaca aatgtttgac tcggggcctt tccacatccg gccttcaggt
      901 aatccttcca gccgtcagcc gaaaggcacc cgcgcgtaat ttacctttta cgtccgttac
      961 gtgcaacgga tgacgtggct gtttttcctt ccccttcgcc atcctttttc ggagcagttg
     1021 tg
//
LOCUS       DAZFLD010000559         1020 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.562, whole genome shotgun sequence.
ACCESSION   DAZFLD010000559 DAZFLD010000000
VERSION     DAZFLD010000559.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1020
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.562"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            173..607
                     /locus_tag="ACHKBL_005221"
     CDS             173..607
                     /locus_tag="ACHKBL_005221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001101430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPD29 domain-containing protein"
                     /protein_id="HFO5821920.1"
                     /translation="MNDNALVNVDDAVVARIRDVLAYTFPGQKFSVRKCDPSFPKVFN
                     NVWVSWLDGPTEAEVRSVTEGFENKDGLLSVLESRYFSDELVQECIDRLRKEYGQGNV
                     PLSVTVERYRKNELEKIETARFPGNISVAINEIGINTSKYRR"
     gene            611..925
                     /locus_tag="ACHKBL_005222"
     CDS             611..925
                     /locus_tag="ACHKBL_005222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5821921.1"
                     /translation="MAGKCLVWFCNGISEPCLIAGNISELLADVTITSNYRGDVTSEI
                     RDVVQEWLRTGRGNLADLKEELWYYNLFINPSADELMNVNRRYGPERTAWLQGFIRNN
                     GA"
BASE COUNT          262 a          214 c          293 g          251 t
ORIGIN      
        1 caggcacgtg aagcatcaga gtatttactg acgcggcgaa cttcgcagga gtgcgcagca
       61 taatttttac taccggtggc ggttataacc tgaccgccat ttatcccgtg agggcattgt
      121 ccctcacggg gcagtgctct ctgttatttc aggattacag aggaactgag tgatgaacga
      181 taacgcactg gttaatgttg acgatgctgt tgtggcccgt atcagggatg tgctggcgta
      241 tacatttccg ggacagaaat tttctgtgcg gaaatgcgat ccatctttcc cgaaagtttt
      301 caacaatgtg tgggtgagct ggctggatgg tccaacggag gccgaagtac gctctgtcac
      361 agaggggttt gagaataaag acgggctgct gtctgtgctt gaatcccgtt atttcagcga
      421 tgaactggta caggagtgta ttgatcgcct tcgtaaagag tacggacagg ggaacgtccc
      481 gttgagtgtg accgtggagc gttaccggaa aaacgagctg gagaagattg aaacagcgcg
      541 ttttccgggc aacatatccg tagccataaa cgagatcggg ataaatacca gcaaatacag
      601 gaggtaaggt atggctggta aatgccttgt ctggttctgt aacgggatct ctgaaccgtg
      661 tctgatagca ggaaatatca gtgaattact ggcagatgtt acgatcacgt caaattaccg
      721 tggagatgtc acatctgaaa tcagggatgt tgtacaggag tggctcagga ccggcagggg
      781 gaacctggcg gatttaaagg aagagctctg gtactacaac cttttcatca atccgtctgc
      841 agacgaactg atgaatgtca acaggcggta tggccctgag cgaactgcct ggctgcaggg
      901 ttttatccgc aacaacgggg cgtgataccc cgttttcccg tgagggcatt gtacctcacg
      961 ggacaatgcc ctctttttac atctgtataa aaaagaggag cgtaaacaat gtctgaatgg
//
LOCUS       DAZFLD010000560         1020 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.563, whole genome shotgun sequence.
ACCESSION   DAZFLD010000560 DAZFLD010000000
VERSION     DAZFLD010000560.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1020
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.563"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(122..925)
                     /locus_tag="ACHKBL_005223"
     CDS             complement(122..925)
                     /locus_tag="ACHKBL_005223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001271558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5821922.1"
                     /translation="MSADGISDLIKPEIFLVIFPGTTTTAKRGGAGWLNNKRVVFRVW
                     LYISKASYKKMGIFQRGSAPESIPYREYHFPSYPDTHEKRKKVLRDWISFMRSRFNIN
                     QIAQFCYRMENEWAFTFDNIHVPRHFREGTDDTEWSWNYIKKKRLFSLSGLQHIQPQN
                     NTERKLLINAFFDTCLIENNPADDIDIKNMLLRKMNAAFYKRKSRARARIHSRNNQQM
                     SVSSGTDKQVNIRLKDFQYQQLLFIAQKENKSKSEIIKELITRAYRAIQ"
BASE COUNT          277 a          198 c          212 g          333 t
ORIGIN      
        1 gcgatgtaac aaaagaatat tcgttatgta tacctggcat agtcagtcgg cacgataaaa
       61 aatagtcagt atcatcccgt gcatatgatg tatggtcggg atgaaatggt gaacgtggtg
      121 gttattgtat cgcccggtat gccctggtaa tgagttcctt tatgatttct gacttacttt
      181 tgttttcttt ctgcgcgata aaaagaagtt gctgatactg aaaatcctta agacgaatat
      241 ttacctgttt atctgttcct gaactgactg acatctgttg gttattccgg gagtgaattc
      301 gcgcacgcgc ccggctcttt cttttataaa acgctgcatt catctttctg agtaacatgt
      361 tctttatgtc aatatcgtca gcagggttgt tttcaataag gcaggtatca aaaaaagcat
      421 taatgagaag ctttcgttca gtattgttct gaggctgaat gtgttgcagg ccgctcaggg
      481 aaaataatcg ttttttctta atgtaattcc atgaccattc tgtgtcatca gttccctccc
      541 gaaagtgtcg tggaacgtgg atattatcaa aggtgaatgc ccattcattt tccatacgat
      601 aacagaactg agctatctga tttatattaa aacgggagcg catgaaggat atccagtcac
      661 gtaatacctt tttccttttt tcatgggtgt cagggtatga gggaaaatgg tattcacgat
      721 agggtattga ttcgggcgca gaaccgcgct gaaaaatccc catcttctta taactggctt
      781 tactgatata aagccatacc cggaacacga ctcttttatt attcaaccac ccggcgccac
      841 cccgttttgc tgtggttgtt gtaccaggaa aaatgacaag aaatatttct ggttttatta
      901 aatcggaaat gccatctgcg ctcatgtttg tacctccatt aaattattaa atactgacat
      961 tttcatgata tgtcagtcaa ttagttttgt gacatgccca tgtcgtctca gaagaacaat
//
LOCUS       DAZFLD010000561         1012 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.564, whole genome shotgun sequence.
ACCESSION   DAZFLD010000561 DAZFLD010000000
VERSION     DAZFLD010000561.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1012
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.564"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            627..>1012
                     /locus_tag="ACHKBL_005224"
     CDS             627..>1012
                     /locus_tag="ACHKBL_005224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO5821923.1"
                     /translation="MGKGGGKGHTPREAKDNLKSTQMMSVIDAIGEGPVEGPVKGLQS
                     ILVNKTPLTDTDGNPVIHGVTAVWRAGEQEQTPPEGFESSGAETALGVEVTKAKPVTR
                     TITSANIDRLRVTFGVQSLVETTSKG"
BASE COUNT          287 a          210 c          308 g          207 t
ORIGIN      
        1 aacattaacg cagcgaaatt ataagcgcta catggatttt gcgaaaatgt gaagaaattc
       61 agaaattttt tgctgcagag gtgcagggag cttgccgggt atttgtggca gaagacagaa
      121 aacatcatgc cggggttaag ggataaaaaa atcccgcaga gtccggagac tgcgggacag
      181 aaccgatgaa gattaacgtt caaggagttt tgtttttatt gacccgaaaa aactgtaacg
      241 ccccggaatg atatctgcca cggggacgta cagaaaatgt gaagaaattc agactttttc
      301 tgtcgcaggt ttacagaacg gatgaccgcc agggtaccag tggcgtaaat gagcaggatg
      361 ttgtggtgtg gctgaagcaa aaaaatctcc cgtaatatcg ccggatactg acgggagacg
      421 aatagtcacc ttaaggaggt ctgataatta ttgcttttat tgagcaaaaa aactgtaacg
      481 ctgcgtcatt atagtggcta caggcaattg ccggaaatgt gaagaatttc agaaatttta
      541 ttccgtcatg acacaggcac cctccggggt gcctgttgtt tttgggcata aacagattca
      601 gacatcagac aggagagggg gacagagtgg gtaaaggggg cggcaagggg cacacaccgc
      661 gtgaggcgaa ggacaatctc aaatccacgc agatgatgag cgtgattgat gccattggtg
      721 agggaccggt ggaaggtccg gtgaagggac tgcagagtat tctggtgaac aaaaccccgc
      781 tgacggacac ggacggtaat cccgtgatac acggtgtgac cgccgtctgg cgtgccgggg
      841 agcaggagca gacaccgccg gaaggctttg agtcctccgg ggcagaaacc gcactgggcg
      901 tggaggtgac gaaggcaaag ccggtgacgc gcaccatcac gtcagcgaac attgaccgtc
      961 tgcgggtcac cttcggggtg cagtcactgg tggagaccac ctcaaagggt ga
//
LOCUS       DAZFLD010000562         1007 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.565, whole genome shotgun sequence.
ACCESSION   DAZFLD010000562 DAZFLD010000000
VERSION     DAZFLD010000562.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1007
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.565"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..484)
                     /locus_tag="ACHKBL_005225"
     CDS             complement(<1..484)
                     /locus_tag="ACHKBL_005225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5821924.1"
                     /translation="MVMVLTGAGMGSAATGGVGMAGASTGTAVFPGAGSRDGDWEEDN
                     ISYPGSGSGSRQGNGGFYYQGKQYDPILNEPMVVSAETHGDFWEDDMDHSETVNYEGI
                     DSKAEVQAKTDEKTCENCPPEGKVMPMIRRCSRWSMVTISYQTRICGTFYNPETQQIQ
                     E"
     gene            complement(484..828)
                     /locus_tag="ACHKBL_005226"
     CDS             complement(484..828)
                     /locus_tag="ACHKBL_005226"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5821925.1"
                     /translation="MLKLSEEQCVGMDKREDDDFVRDTALRLKKKFPGITESDDTFHV
                     RLRKALDYANTLPLKEKNVKRDFLFLEAFWPGFYLKPEVDKWLRTPNAYSTDQRVEDY
                     KHVMINRERREL"
     gene            complement(822..>1007)
                     /locus_tag="ACHKBL_005227"
     CDS             complement(822..>1007)
                     /locus_tag="ACHKBL_005227"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5821926.1"
                     /translation="MNTELPDGRIALLRFHDPRVQKRLGEMLNDQQHRELTGLMQEWL
                     TIVDGKAWSFKQREFIC"
BASE COUNT          227 a          303 c          182 g          295 t
ORIGIN      
        1 attcctgtat ctgttgcgtc tccggattat aaaacgtgcc acatattctt gtctggtagc
       61 tgattgttac catactccag cgcgagcaac gccttatcat cggcattacc ttcccttccg
      121 gtgggcagtt ctcacacgtt ttctcatccg tcttcgcctg tacttcggct ttgctatcaa
      181 taccttcata gtttactgtt tctgaatgat ccatatcatc ttcccagaaa tcaccatgcg
      241 tctctgcgct gacaaccatc ggctcattca gaattggatc atactgtttc ccctgataat
      301 aaaaccctcc gttaccctga cgcgaaccac tgcctgagcc aggataagat atattatctt
      361 cttcccagtc tccatcgcgg cttcctgctc cgggaaatac tgctgttccg gttgaggcac
      421 cagccatacc aacacctccg gtagctgcgc tccccatacc agcccctgtc aggaccataa
      481 ccatcagagc tccctccttt ctctgtttat catcacatgc ttataatctt caacccgctg
      541 gtcagttgaa taagcattcg gcgttctcaa ccatttatcc acttcgggtt tcagataaaa
      601 accaggccag aaggcctcca gaaacaagaa gtcccgcttc acgtttttct ctttaagcgg
      661 aagggtattg gcataatcca gcgcttttct caggcgaaca tggaacgtgt catctgattc
      721 agtaattccc ggaaatttct tcttcagtct tagcgcggtg tcgcgaacaa aatcatcatc
      781 ttcccttttg tccataccaa cacactgctc ttcagacaac ttcagcatat gaactccctt
      841 tgtttaaatg accaggcttt tccgtccact attgtcagcc actcctgcat taaccccgtc
      901 agctcccgat gttgttggtc gttcagcatt tctcccaggc gcttctgaac gcgcggatca
      961 tggaaacgca gcaacgcaat ccgtccatcc ggtagttctg tattcat
//
LOCUS       DAZFLD010000563         1000 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.566, whole genome shotgun sequence.
ACCESSION   DAZFLD010000563 DAZFLD010000000
VERSION     DAZFLD010000563.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1000
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.566"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            132..686
                     /locus_tag="ACHKBL_005228"
     CDS             132..686
                     /locus_tag="ACHKBL_005228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000478924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFO5821927.1"
                     /translation="MRLTPMGTPSKAPAHVKPWTQQEDRLLISLYQEHNTHQLAERIQ
                     RTRKAVMHRIRFLRNRGLIGRKRKAPLSTEVIAFLIKNRHVKTAQEMALEAGCSECTV
                     RYTLRKRGYNLQKCGENHHCARYSDHLVELVTELRDIHNMTFCMIAEHINSTTQMHIN
                     DDTAFHLYHRRTATDAVLCELLPD"
     gene            696..1000
                     /locus_tag="ACHKBL_005229"
                     /pseudo
     CDS             696..1000
                     /locus_tag="ACHKBL_005229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001310326.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ash family protein"
BASE COUNT          278 a          275 c          245 g          202 t
ORIGIN      
        1 acgctgagcg gtccgggcgc accagtaaca ccaggtgaga tccaccggga ccttaaacgc
       61 cgtattgaaa acgccattga ttacaccctg aacccgaccg aataaccgga acagtaacgg
      121 aggttatcgc gatgagactt acaccaatgg gaacgccaag caaagctccg gcacatgtaa
      181 agccgtggac acaacaggaa gatcggttac tcatctcgct ttatcaggaa cacaacacac
      241 atcagctcgc cgaacgaatt cagcgaaccc gtaaagcagt tatgcaccgg attcgttttt
      301 tacgtaatcg tgggctgatt ggccgcaaga gaaaagcacc actgagtaca gaagtcatag
      361 cctttcttat taaaaaccgt catgtaaaaa ccgcacagga aatggctctg gaagcgggat
      421 gcagtgagtg cacagtgagg tacacactgc gtaaacgggg ttacaacctg caaaaatgcg
      481 gtgagaacca ccattgcgcc agatacagcg atcatctggt tgagctggtc acggagctac
      541 gcgacataca caacatgacg ttctgcatga tcgctgaaca catcaacagc acgacacaaa
      601 tgcacattaa cgacgatacg gcgtttcatc tctaccaccg ccgcaccgca actgacgcag
      661 tgctctgtga actgttaccg gactgagtga tcatcatgca cacacaaaaa aaccgcttgc
      721 cagcgttagc ctggtcgggt tacagttttc ccgcacctca taaaacgggt gccgggattg
      781 caacccccgc tgactacaca agcgcacaac cgcgccagcg gtttttttgt gcgtaccgca
      841 tcgtcacatc tttttcgcgt cagaattatg gtggggcgta cagggccgac ttcggtcggg
      901 ccggggtctt gtgtagccgg tggttgcaac cctgtacgtc tcaccaccca gaattgcaac
      961 ctctggatgg tgagttttca aaacttacta cacaagaggc
//
LOCUS       DAZFLD010000564          999 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.567, whole genome shotgun sequence.
ACCESSION   DAZFLD010000564 DAZFLD010000000
VERSION     DAZFLD010000564.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 999)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..999
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.567"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..357
                     /locus_tag="ACHKBL_005230"
     CDS             <1..357
                     /locus_tag="ACHKBL_005230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001534332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO5821928.1"
                     /translation="HTHGGNISNTDLGTKTTSSFDYGTKTTSTNGQHEHSVPLRRQDG
                     GGINFDWLDGSSSGSHIGNGYVPAAGNHQHTIPIGAHTHTLALGSHGHGLTINPTGNA
                     ENTVKNIAFNYIVRLA"
     gene            357..953
                     /locus_tag="ACHKBL_005231"
     CDS             357..953
                     /locus_tag="ACHKBL_005231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000143164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFO5821929.1"
                     /translation="MTFKMSEHPQTIKIYNLSAGTNEFIGEGDAWIPPHTGLPAHSTD
                     IAPPDIPEGHVAIFDEDENTWHLMEDHRGKTVWRLESGSALLITEPGPLPENVTMIKP
                     DGEFCRWDGSSWVKDEAAEQAYRVSEAENKKRELIQEATNTINILQDAVDLNMATDDE
                     SALLPLWKKYRVLLSRTDTGNAPDIVWPESPSLDHHAG"
BASE COUNT          298 a          269 c          246 g          186 t
ORIGIN      
        1 cacacccacg gagggaacat ctcaaatact gaccttggca cgaaaacgac ctcatcattt
       61 gattacggaa ccaaaacaac ctctacgaac ggacaacatg aacacagcgt ccccctgaga
      121 agacaagatg gcggaggcat aaactttgac tggctggatg gttcatcgtc tggttctcat
      181 attggtaatg gatacgttcc tgccgcaggt aaccaccaac ataccatacc cattggcgca
      241 cacacacaca cgctcgcact gggttcacat ggtcacggcc tgaccattaa ccccaccggt
      301 aatgcggaaa acaccgtcaa aaacatcgca tttaactaca tcgtgaggct tgcataatga
      361 cctttaaaat gagcgaacac ccacagacaa ttaaaatcta caacctgtca gccggaacaa
      421 atgaatttat cggcgaaggc gatgcatgga tccccccgca taccggactg cctgcgcact
      481 caacggacat tgcccctcct gacattcctg aaggacacgt ggctatcttt gatgaggatg
      541 aaaacacctg gcacctcatg gaagatcacc ggggtaaaac cgtctggcgt ctggagtcgg
      601 ggagcgcctt actgattact gagccgggcc cgttaccgga aaacgtgacc atgataaaac
      661 cggacgggga gttctgccgc tgggacgggt catcatgggt taaggatgaa gcggcagaac
      721 aggcttaccg ggtaagcgaa gcggagaata aaaaacggga attaatacag gaagcaacaa
      781 atacaataaa catccttcag gatgccgtgg atttaaatat ggcgaccgac gatgaaagcg
      841 ccctgcttcc cctgtggaaa aaataccggg tattactgag ccggacagac acgggcaacg
      901 cgccggatat tgtctggcct gaatcccctt cgctggatca tcacgcaggc tgaccggagg
      961 caccatgacg tggacaagaa aaacaatcag cgtcagcgc
//
LOCUS       DAZFLD010000565          998 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.568, whole genome shotgun sequence.
ACCESSION   DAZFLD010000565 DAZFLD010000000
VERSION     DAZFLD010000565.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..998
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.568"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..989)
                     /locus_tag="ACHKBL_005232"
     CDS             complement(<1..989)
                     /locus_tag="ACHKBL_005232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003838074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019062 - virion attachment to host cell
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HFO5821930.1"
                     /translation="MDERQRRENVDIYGKATFFDDGWLVKKGETWTVNKGVGYVEGLR
                     AELTEDTPMVTPTSPSRIWLNVWWTGTLTSEWTVEHEIIPADTDVTEERLRKTYPRDG
                     IQQYFYAIAEIDEDGNVTDLRPRGTPEERRSLDALKKHEKSRNHPDATLKEKGFTQLS
                     SATTSTSETQAATPKAIKIAMDNADGRLAKNRNGEDIPNKELFLINVGAPRVYAKDGY
                     VGEAGQRWTTEQFVEWLNERGAFRPGLWIFKANISLSRHSVLTDTGYGDICLGGCLIE
                     VSGDINGGIIRITTGLESPNPADMYLRTQFTGVVLNKGKSTIWYRTYTDKNKPT"
BASE COUNT          168 a          273 c          268 g          289 t
ORIGIN      
        1 gtgggtttgt ttttgtccgt gtaagtgcgg taccagatgg tgcttttccc tttatttaaa
       61 acaaccccgg tgaactgtgt gcggaggtac atgtcggcag ggtttgggct ttcgagacct
      121 gttgtgatcc tgatgatccc cccgttgatg tcgcctgata cttcgataag gcagcctcca
      181 aggcagatat caccatagcc cgtatcagtg agcacgctgt gccgggaaag tgaaatattg
      241 gctttgaata tccagagtcc tggtctgaag gcgccacgct cattcagcca ttccacgaat
      301 tgttctgttg tccagcgttg ccctgcttca cccacgtacc cgtcttttgc ataaacgcgt
      361 ggtgcgccca cgttgatcag gaacagctct ttgttgggga tgtcctcacc attgcggttt
      421 ttcgcaagcc gaccatcggc gttgtccatg gcgatcttta tagcctttgg cgtggctgcc
      481 tgtgtctctg atgtactggt ggtggcgctg cttaactgtg tgaagccctt ttctttcagc
      541 gttgcgtccg ggtggttgcg tgatttctcg tgttttttca gggcgtccag actgcggcgc
      601 tcttccgggg ttcctctggg gcgcaggtca gtcacgttgc cgtcttcatc aatttcagcg
      661 atggcataaa aatattgctg aatgccgtcc ctgggatatg ttttccgtaa acgctcctcc
      721 gtaacgtccg tgtcggctgg tatgatttcg tgctccaccg tccattcact ggtgagcgta
      781 ccggtccacc acacattcag ccagatacgg gacggggagg tgggcgtgac cattggtgtg
      841 tcttcggtca gctctgcgcg tagcccctcc acgtaaccca cccctttgtt gactgtccag
      901 gtttcgcctt tcttaaccag ccacccgtca tcaaaaaaag ttgccttccc gtagatatcg
      961 acgttttccc ggcgctggcg ttcgtccatg gctgccag
//
LOCUS       DAZFLD010000566          996 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.569, whole genome shotgun sequence.
ACCESSION   DAZFLD010000566 DAZFLD010000000
VERSION     DAZFLD010000566.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 996)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 996)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..996
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.569"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(15..650)
                     /locus_tag="ACHKBL_005233"
     CDS             complement(15..650)
                     /locus_tag="ACHKBL_005233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000203832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antA/AntB antirepressor family protein"
                     /protein_id="HFO5821931.1"
                     /translation="MTSQLIPVFNGSISNQAALLCNARNLHAFLGVGKRFASWITERI
                     AEYGFVENQDYMIASQIREAKGRGGHNRKDYHLTLDTAKELAMVERNEKGRQIRRYFI
                     ECEKKFRQSLLPAPMNINYPLSWFSEHHPYSMMSYVDRKTLNLDVSVLFDMPSPTMRI
                     LNELHSKGYNVDAAVAEFNAFKHLTEEMRRTLQDISRLSDRNSRKGFSLSL"
BASE COUNT          247 a          200 c          261 g          288 t
ORIGIN      
        1 gatggtaggg gaagttacag gcttaacgag aagccttttc gggaatttcg atctgacagt
       61 cttgaaatat cctgtagcgt gcggcgcatt tcttccgtca gatgtttgaa ggcgttaaat
      121 tcggcgacag cggcgtcaac gttatagccc ttgctgtgta gctcgttgag gatgcgcata
      181 gttgggcttg gcatatcgaa gagcacagaa acgtccaggt taagagtttt tcgatcaaca
      241 tagctcatca tggagtaggg gtgatgctct gaaaaccacg agagagggta gttgatgttc
      301 attggtgcag gtaaaaggct ctggcgaaac tttttctcgc actcgatgaa gtagcggcgg
      361 atctggcgac ctttttcgtt acgttcaacc atcgcaagct ctttggctgt gtccagggtg
      421 aggtggtagt ccttgcggtt gtggccgcct ctaccttttg cttcccgaat ttgggaagca
      481 atcatatagt cctgattttc aacgaaacca tattcagcaa tacgttctgt tatccacgat
      541 gcaaagcgtt taccaacccc aaggaaagcg tgtagattgc gagcattgca tagaagggct
      601 gcttggtttg agatagaacc gttgaatacg gggatgagtt gactagtcat ttttatgtcc
      661 ttgctgattt gttcagataa ccctgtgttc agcagggtgg tcaggtactt gaacaccgca
      721 gcaagtcggc ccgtatcctt agccttacgg ctgtttttcg gtatacgcgc tacccgacca
      781 tatctgaaaa atggacacaa aaaaaaccgc atgactgacg gggcggtttc cgcttgctgt
      841 ggtgtgttca gcaccatgaa gaggagtatc cgacagaatg tgtggcgtgt caacatttcc
      901 ttgtgtagat gttgacaccc tattaggtaa aacattaacg ttgttttaag ttgttaataa
      961 ttatcaattt aatattaatg tttggagtat aaaagc
//
LOCUS       DAZFLD010000567          994 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.570, whole genome shotgun sequence.
ACCESSION   DAZFLD010000567 DAZFLD010000000
VERSION     DAZFLD010000567.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..994
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.570"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..158)
                     /gene="ssb"
                     /locus_tag="ACHKBL_005234"
     CDS             complement(<1..158)
                     /gene="ssb"
                     /locus_tag="ACHKBL_005234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFO5821932.1"
                     /translation="MASRGVNKVILIGHLGQDPEIRYFPNGGGAVANLTLATTDTWRD
                     KQSGEMKE"
     gene            complement(174..377)
                     /locus_tag="ACHKBL_005235"
     CDS             complement(174..377)
                     /locus_tag="ACHKBL_005235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5821933.1"
                     /translation="MKPTSGILAAKAGYTLPLQILSSARGYYIGTQCDEGPVSRESEE
                     YFRNYEQAVRALENGIWTQRECV"
     gene            complement(468..935)
                     /locus_tag="ACHKBL_005236"
     CDS             complement(468..935)
                     /locus_tag="ACHKBL_005236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016151303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STY4534 family ICE replication protein"
                     /protein_id="HFO5821934.1"
                     /translation="MTANTSSANAKTEYFNLTLKGLGYLSNIRQVNHQNGTFLSCVVN
                     ALSGPTDSPSYVRFDVTVAGKEATSLIARCQKAVDEDRKVLIGFTMSNPSTDIFTLNR
                     GEHAGEQRVSLKARLIKVEWIKIGQDMVYKTEKSESIPPQNGNTPREYAENSF"
BASE COUNT          245 a          258 c          222 g          269 t
ORIGIN      
        1 tgttccttca tttcaccgct ctgtttatca cgccaggtat cagtggtggc cagcgtcaga
       61 tttgccactg cacctccgcc gttggggaaa taacggattt ccggatcctg tcccagatga
      121 ccaatcagta tgactttatt cactccacgc gatgccataa caactcctta tcctcacaca
      181 cattcacgct gagtccagat gccgttttcc agagcccgga ctgcctgctc ataatttctg
      241 aaatactctt cggattcccg ggacacaggc ccttcgtcgc actgagtacc aatataataa
      301 ccacgagcac tgctgaggat ctgcagaggt aaggtatagc ccgctttggc ggccagaatt
      361 ccgcttgtcg gtttcataac atgtttcctt aaagttaaac gataagaaaa cagacctccc
      421 tgatggttgg tctgttttca tcagggaggt tgagttgaaa aatacgatca gaaagaattt
      481 tctgcgtatt cccgcggggt attgccattc tgtggcggta tggattccga tttttcagtc
      541 ttataaacca tgtcctggcc tattttgatc cactccacct tgatcaggcg ggctttcaga
      601 ctgacgcgtt gctcaccggc atgttcaccc cggttaaggg taaaaatgtc agtagagggg
      661 ttgctcatgg taaaaccaat cagcaccttg cgatcttcat ccaccgcttt ctggcagcgg
      721 gcaatcaggg aagttgcctc tttaccggca accgtcacat caaaacgcac ataggacggg
      781 ctgtccgtcg gaccactcag tgcattcaca acacaactga gaaatgtccc gttctgatga
      841 ttaacctgac gaatgttgct gagatacccc aggcctttca gtgtcagatt gaagtactca
      901 gttttagcgt ttgcagaaga agtatttgca gtcataatga tatctccatt ctcagtatcg
      961 gaaagacaga gaacatcatc atccctgacg ggtt
//
LOCUS       DAZFLD010000568          990 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.571, whole genome shotgun sequence.
ACCESSION   DAZFLD010000568 DAZFLD010000000
VERSION     DAZFLD010000568.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..990
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.571"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(409..981)
                     /gene="hdeD"
                     /locus_tag="ACHKBL_005237"
     CDS             complement(409..981)
                     /gene="hdeD"
                     /locus_tag="ACHKBL_005237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-resistance protein HdeD"
                     /protein_id="HFO5821935.1"
                     /translation="MLYIDKATILKFDLEMLKKHRRAIQFIAVLLFIVGLLCISFPFV
                     SGDILSTVVGALLICSGIALIVGLFSNRSHNFWPVLSGFLVAVAYLLIGYFFIRAPEL
                     GIFAIAAFIAGLFCVAGVIRLMSWYRQRSMKGSWLQLVIGVLDIVIAWIFLGATPMVS
                     VTLVSTLVGIELIFSAASLFSFASLFVKKQ"
BASE COUNT          343 a          224 c          192 g          231 t
ORIGIN      
        1 aaattacctg attaaataac tcattcatgt cttcaggttt ttatatagcg tctggagaca
       61 cggatattta tgcaatgtaa tcaacgccag ttgcaaaaaa atgaacccgg attacttatt
      121 taaccgccca tagcatcaac gcttacggaa ctctttgttc actttcccac agcactttta
      181 aaacattaaa cctgaccacg gaaaatatca gccatgataa tgtttgaaat ggctaatttg
      241 ccatggaacg aaaaaaatca gattaaattc agtaggttga aataatcaac agcaggcaat
      301 tgtttcaatg atagtgcgta attgaactga aacactgtat agctaagaga gtattaactg
      361 gatcatagtc acttaatgtg actatgatcc aggtggaaat agggataatt attgcttttt
      421 aacgaacaaa ctggcgaagc tgaacagact ggcggcgcta aatatcagtt caattcccac
      481 cagtgtggaa accagcgtta ccgacaccat cggcgtagca ccaaggaata tccaggcaat
      541 gacgatatcc agcacaccaa taacgagctg tagccaactg cctttcattg aacgctgacg
      601 ataccaactc atcaggcgaa taacccctgc cacacagaac aaaccggcaa taaatgccgc
      661 aatggcaaaa atgcccagct ctggtgcgcg gatgaagaaa tagccgatca ataaataggc
      721 gactgcgacg aggaaaccgg ataataccgg ccagaaatta tgactgcggt tgctgaatag
      781 cccgacaata agcgcaatgc ccgagcagat taataatgca cccactactg tgcttaaaat
      841 gtcgccagag acgaacggaa aactgataca cagcaacccg acgataaaca gcagcacggc
      901 aataaactgg attgctctgc gatgtttttt aagcatctcc agatcaaact tcaaaattgt
      961 tgccttatct atatataaca tagaaccacc
//
LOCUS       DAZFLD010000569          990 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.572, whole genome shotgun sequence.
ACCESSION   DAZFLD010000569 DAZFLD010000000
VERSION     DAZFLD010000569.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..990
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.572"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..539)
                     /locus_tag="ACHKBL_005238"
     CDS             complement(<1..539)
                     /locus_tag="ACHKBL_005238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFO5821936.1"
                     /translation="MTQTESAILVHARRCAPAESCGFVISTPEGERYQPCVNISAEPE
                     AYFRIAPEDWLRAEMQGEIVALVHSHPGGLPWLSEADRRLQIKSALPWWLVCRGDIHR
                     FRCVPHLTVRRFEHGVTDCYTLFRDAYHLAGITLPDFVREDDWWRNGQNLYLDNLADN
                     GFYRVSPSCAQAGDILLCC"
     gene            complement(550..>990)
                     /locus_tag="ACHKBL_005239"
     CDS             complement(550..>990)
                     /locus_tag="ACHKBL_005239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFO5821937.1"
                     /translation="LVTGMAEDLQSLVGAPVVRRRVYARFLDAVNFVAGNPEADPEQE
                     LTDRWVVEQMSALTAMTASFVLATPTETDGALFPGRIMLANTCMWDYRGDECGYNGPA
                     VADEFDNPTTDIRKDRCSKCMRGCEMRGMVANFGGFLSINKLSQ"
BASE COUNT          212 a          347 c          256 g          175 t
ORIGIN      
        1 aagcagcaca gcaggatatc gcccgcctgt gcacaggacg gagacacccg gtaaaagccg
       61 ttgtccgcca ggttgtccag gtacaggttc tggccgttgc gccaccagtc atcctcacgc
      121 acaaaatccg gcagcgttat ccccgccaga tggtatgcat cccggaacag ggtgtaacag
      181 tccgtcacac cgtgttcaaa gcgccgtacg gtcaggtgcg gcacacagcg gaaccggtga
      241 atgtcacccc ggcagaccag ccaccagggc agggcactct ttatctgcag cctccggtcc
      301 gcctcgctca gccagggcag accaccggga tgactgtgga ccagcgccac aatctccccc
      361 tgcatctctg cccgcagcca gtcttccggt gcgatacgaa aatacgcctc cggctctgcg
      421 gagatattca cgcagggctg gtaccgttcg ccctccgggg tgcttatcac gaagccgcac
      481 gactccgctg gcgcacaccg ccgggcatgc accagaatcg ctgattcagt ctgtgtcata
      541 aaacgggatt tactgcgaaa gtttattaat ggaaaggaaa ccgccgaaat tagccaccat
      601 gccgcgcatc tcacacccgc gcatgcattt actgcatctg tccttccgga tatccgtggt
      661 agggttgtcg aactcatccg ccactgccgg accgttatac ccgcactcat cgccccggta
      721 atcccacata caggtgttcg ccagcatgat acgaccggga aacagcgccc cgtccgtctc
      781 ggtcggtgtc gccagcacaa acgaggccgt catggccgtc agcgctgaca tctgctccac
      841 cacccaccgg tccgtcagct cctgctccgg gtctgcctca ggattgcctg ccacaaaatt
      901 caccgcatcc agaaaacgcg catacacccg gcggcggacc accggggcac ccaccaggct
      961 ctgcaaatcc tccgccatcc cggtgacaag
//
LOCUS       DAZFLD010000570          990 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.573, whole genome shotgun sequence.
ACCESSION   DAZFLD010000570 DAZFLD010000000
VERSION     DAZFLD010000570.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..990
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.573"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..539)
                     /locus_tag="ACHKBL_005240"
     CDS             complement(<1..539)
                     /locus_tag="ACHKBL_005240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFO5821938.1"
                     /translation="MTQTESAILVHARRCAPAESCGFVISTPEGERYQPCMNISAEPE
                     AYFRITPEDWLQAQMQGEIVALVHSHPGGLPWLSEADRRLQIKSALPWWLVCRGEIHR
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGITLPDFVREDDWWRNGQNLYLDNLAEN
                     GFYRVSPSCAQAGDILLCC"
     gene            complement(550..>990)
                     /locus_tag="ACHKBL_005241"
     CDS             complement(550..>990)
                     /locus_tag="ACHKBL_005241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFO5821939.1"
                     /translation="LVTGMAEDLQSLVGATVVRRRVYARFLDAVNFVAGNPEADPEQE
                     LTDRWVVEQLSELTAMTASFVLATPTETDGALFPGRIMLANTCMWDYRGDECGYNGPA
                     VADEFDNPTTDIRKDRCSKCMRGCEMRGMVANFGGFLSINKLSQ"
BASE COUNT          213 a          344 c          252 g          181 t
ORIGIN      
        1 aagcagcaca gcaggatatc gcccgcctgt gcacaggacg gagacacccg gtaaaagccg
       61 ttttccgcca ggttgtccag gtacaggttc tgaccgttgc gccaccagtc atcctcacgc
      121 acaaaatccg gcagcgttat ccccgccaga tggtatgcat cccggaacag ggtgtaacag
      181 tccgtcacac cgtgttcaaa gcgccgtccg gtcaggtgcg gcacacagcg gaaccggtga
      241 atttcacccc ggcagaccag ccaccagggc agggcactct ttatctgcag ccgccggtcc
      301 gcctcgctca gccagggcag accaccggga tgactgtgga ccagcgccac aatctccccc
      361 tgcatctgtg cctgcagcca gtcttccggt gtaatacgaa aatacgcctc cggctctgcg
      421 gagatattca tgcagggctg gtaccgttcg ccctccgggg tgcttatcac gaagccgcac
      481 gactccgctg gcgcacaccg ccgggcatgc accagaatcg ctgattcagt ctgtgtcata
      541 aaacgggatt tactgcgaaa gtttattgat ggaaaggaaa ccgccaaaat tagccaccat
      601 gccgcgcatc tcacacccgc gcatgcattt actgcatctg tccttccgga tatccgtggt
      661 ggggttgtcg aactcatccg ccactgccgg accgttatac ccgcactcat cgccccggta
      721 atcccacata caggtgttcg ccagcatgat gcgaccggga aacagcgcac cgtccgtctc
      781 cgtcggtgtc gccagcacaa acgaggccgt catggccgtc agctctgata actgctccac
      841 cacccaccgg tccgtcagct cctgctccgg gtctgcctca ggattgcctg ccacaaaatt
      901 caccgcatcc agaaaacgcg catacacccg gcgacggacc accgtggcac ccaccaggct
      961 ctgcaaatcc tccgccatcc cggtgaccag
//
LOCUS       DAZFLD010000571          990 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.574, whole genome shotgun sequence.
ACCESSION   DAZFLD010000571 DAZFLD010000000
VERSION     DAZFLD010000571.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..990
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.574"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..695
                     /locus_tag="ACHKBL_005242"
     CDS             <1..695
                     /locus_tag="ACHKBL_005242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DnaB-like helicase C-terminal domain-containing
                     protein"
                     /protein_id="HFO5821940.1"
                     /translation="LRRTEESVLVFSIEMPKEQLILRMLSMLGRVELNHLRSGNMDDE
                     DWLRVSSAVGMALGDGGMGSLGERIIIDDCCEQTPSSLRAGARRYTRLYGKPSLIMVD
                     YLQLIRAPELENRTQEIAEISRSLKALGKELGCPVLALSQLNRQVEQRADKRPNNGDL
                     RDSGALEQDADLIMFIYRDEVYNPDSLAKGEAEIIVSKQRQGPTGTVRVRFDGRYTLF
                     TPSENDGPGGYA"
     gene            695..>990
                     /locus_tag="ACHKBL_005243"
     CDS             695..>990
                     /locus_tag="ACHKBL_005243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partitioning protein ParB"
                     /protein_id="HFO5821941.1"
                     /translation="MAHKPLDLGAAIMQPGRQTTGAGNVVAMGETPMVLTLDQLRPNP
                     DNPRTTRNPRYDDIKNSIHARGLDTVPKVTRMPDSEPDVYIFSDGGNTRYQILT"
BASE COUNT          220 a          251 c          309 g          210 t
ORIGIN      
        1 cactgcggcg aacggaagag agcgttctgg tattcagcat tgagatgccg aaggagcaac
       61 tgattctgcg catgctttcc atgctggggc gtgtggaact gaatcatttg cgcagtggca
      121 acatggatga tgaggactgg ctgagggtgt catctgcggt cggtatggcg cttggtgacg
      181 gaggaatggg ctctctgggt gagcgcataa taattgatga ctgctgtgag cagacgcctt
      241 catcactgcg tgcaggcgcc cgtcgttata cacgtctgta cggtaaaccc tccctgatta
      301 tggtggatta tctgcaactg attcgtgcgc cggagctgga aaaccgcaca caggaaattg
      361 ctgaaatttc ccgttccctg aaagcgctgg ggaaggagct gggctgcccg gtgctggccc
      421 tctcccagct taaccggcag gtggagcagc gtgctgataa acgccccaat aacggtgacc
      481 tgcgtgattc cggggcgctg gagcaggatg ctgacctgat tatgtttatt taccgcgacg
      541 aggtctataa ccccgactca ctggcaaagg gggaggcaga aatcattgtc agcaagcagc
      601 gtcaggggcc cacgggtacg gtacgggtca ggttcgacgg tcgttacaca ctgtttacgc
      661 cctctgagaa tgacgggccg ggaggatatg cgtgatggca cataaaccac tggatctcgg
      721 ggcggctatt atgcagcccg gtcgtcagac cactggtgcc ggcaatgtgg tggcaatggg
      781 ggaaacgccc atggttctga cgctggacca gttacgtccg aatccggata acccgcgcac
      841 cacgcgcaat ccgcgttatg acgatatcaa aaattccatt catgcccgcg ggctggatac
      901 ggtgcccaaa gtcacccgta tgccggacag tgagccggat gtgtatatct tcagtgacgg
      961 gggaaacacc cgttaccaga tactgacgga
//
LOCUS       DAZFLD010000572          984 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.575, whole genome shotgun sequence.
ACCESSION   DAZFLD010000572 DAZFLD010000000
VERSION     DAZFLD010000572.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 984)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 984)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..984
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.575"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..353)
                     /gene="tssK"
                     /locus_tag="ACHKBL_005244"
     CDS             complement(<1..353)
                     /gene="tssK"
                     /locus_tag="ACHKBL_005244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004171008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
                     /protein_id="HFO5821942.1"
                     /translation="MKIFRPLWSEGVLLSPQQFQQQAEWESFRSAGISALASPFPWGV
                     EQVEFNDSLLSSGLIQITQLRLWLEDGTLIDAQRSDLPPAPRELDAGQLADRDAVTVV
                     IALPHMQPGVANVEQ"
     gene            complement(369..>984)
                     /gene="tssC"
                     /locus_tag="ACHKBL_005245"
     CDS             complement(369..>984)
                     /gene="tssC"
                     /locus_tag="ACHKBL_005245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
                     /protein_id="HFO5821943.1"
                     /translation="RSFVTNGWCVQIRGPQAGGAVQDLPIHLYDLGTGNQVKIPSEVM
                     IPETREFEFSNLGFIPLSYYRNRDYACFFSANSAQKPAQYDTPDATANSRINARLPYI
                     FLLSRIAHYLKMIQRENIGTTKDRRLLELELNTWLRGLVTEMTDPGDELQASHPLRDG
                     KVVVEDIEDNPGFFRVKLYAIPHFQVEGMDVSLSLVSQMPKAKA"
BASE COUNT          244 a          286 c          247 g          207 t
ORIGIN      
        1 tcctgctcaa cgttggcaac gcctggctgc atgtggggaa gtgcaataac gacagttacc
       61 gcatcgcggt cagccagctg accggcatct aactcacgtg gcgccggagg cagatcgctc
      121 ctttgagcat caataagtgt tccgtcttcc agccagaggc gtaactgcgt aatctggatc
      181 agcccggacg acagaagact gtcattaaac tcaacctgtt ccactcccca gggaaacggg
      241 gatgccagcg cagagatacc ggcactgcga aaagactccc attcagcctg ctgctgaaac
      301 tgctgagggg agagcaaaac accttcgctc cacagcggac ggaaaatctt catttcccga
      361 cacctgcctt acgcttttgc cttcggcatc tgcgaaacca gggacaggct gacatccatt
      421 ccttccacct ggaaatgcgg aatggcatac aatttcacac ggaagaaccc cgggttatct
      481 tcaatatctt ccaccaccac ttttccgtca cgcagcggat gcgatgcctg aagctcatcg
      541 cccggatcgg tcatctccgt caccaggccc cggagccagg tattcagctc cagctccagc
      601 agacgccgat ctttggtggt accgatattt tcccgctgaa tcatcttcag gtaatgcgca
      661 atgcgggaca gcaggaaaat atagggaaga cgggcattta tacggctgtt cgctgtcgca
      721 tccggcgtat cgtactgtgc cggtttctgg gcggagttag cagagaaaaa gcaggcataa
      781 tcccggtttc tgtagtaaga gagcggaata aagcccagat tagaaaactc aaattcccgc
      841 gtttcaggga tcattacctc tgacggtatt ttgacctgat taccggtccc caaatcatac
      901 agatggatcg gcaaatcctg caccgcacca ccggcctgag gaccgcggat ctgaacacac
      961 cagccgttgg tgacaaaact gcgc
//
LOCUS       DAZFLD010000573          983 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.576, whole genome shotgun sequence.
ACCESSION   DAZFLD010000573 DAZFLD010000000
VERSION     DAZFLD010000573.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..983
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.576"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..621
                     /locus_tag="ACHKBL_005246"
     CDS             <1..621
                     /locus_tag="ACHKBL_005246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001681749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbC"
                     /protein_id="HFO5821944.1"
                     /translation="GEFFTVFQGDVVPSASVYIPDSEKSCDSDPVVINRYISVEAPRL
                     ERLRRLVPRTVQRRLPTPEHVSSIIGVLTAKPSRKRRKNRTEPYRIIDTFQRQLATAQ
                     TSLDLLPQYDTDIESRANELWKKAVHTINNTTREERRVCYITLNRPEEEHSGPEDIPS
                     VKAILNTLLPLEMLLPVPDLTASPPHKKNVAQTPSGGKQESRKKRF"
     gene            735..>983
                     /locus_tag="ACHKBL_005247"
     CDS             735..>983
                     /locus_tag="ACHKBL_005247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001402879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5821945.1"
                     /translation="MCISSPETNSWSVIYRKNSGEDINITSLTFKNSLLAARTLMVPE
                     NYMICILRNGERVRRWDREILAGSNRWYKCSPDNFEILG"
BASE COUNT          288 a          245 c          224 g          226 t
ORIGIN      
        1 ggtgagttct ttaccgtttt ccagggggat gtggtgccgt cagcctctgt gtatattccg
       61 gacagcgaga aaagttgcga cagtgatccg gtggtcatca accgctatat ttccgttgaa
      121 gccccccggc tggagcgtct ccgccgtctc gtccccagga cagtgcagcg caggctcccc
      181 acaccggagc atgtcagcag catcatcggt gtactgacag caaaaccatc ccgtaaacgt
      241 cgtaaaaacc gtacagagcc ttaccgtatc attgatacat tccagcgcca gttggcaaca
      301 gcgcagacat cactggatct gcttccgcaa tatgatacag atatcgaatc ccgggcaaac
      361 gaactgtgga aaaaggctgt gcacactatc aataacacaa ccagggaaga acgccgggta
      421 tgttatatca cactgaatcg tccggaagag gaacactccg gtcctgaaga tataccttca
      481 gtgaaggcga ttctgaatac tctgctcccc ctggagatgc tgttacctgt accggatcta
      541 actgcatccc cgccccataa aaaaaatgtc gctcagacac catcaggtgg aaaacaagag
      601 agtcgtaaaa aaagattctg ataacacctg tatggctgat tttaataaca acctttacgc
      661 gattattaat ctcctccgct caccatagta agcggaggat tcaggatttc aatccatcac
      721 cagaggtaca ataaatgtgt attagtagcc cggaaacaaa ctcatggtct gttatttatc
      781 gtaaaaattc cggagaagat atcaatatca cttccctgac gtttaaaaat agtcttctgg
      841 cagcccggac actgatggtg cccgaaaact atatgatatg catactcaga aacggggaga
      901 gggtcagacg atgggacaga gaaatacttg caggctcaaa cagatggtac aagtgttctc
      961 ccgataattt cgaaatactg ggt
//
LOCUS       DAZFLD010000574          980 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.577, whole genome shotgun sequence.
ACCESSION   DAZFLD010000574 DAZFLD010000000
VERSION     DAZFLD010000574.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..980
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.577"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..573
                     /locus_tag="ACHKBL_005248"
     CDS             <1..573
                     /locus_tag="ACHKBL_005248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HFO5821946.1"
                     /translation="MLRLRRAGQITGQHVPEIILLNSHDGTSSYQMLPGYFRAICTNG
                     LVCGQSLGEVRVPHRGNVVDRVIEGAYEVVGVFDRIEEKRDAMQSLVLPPPARQALAQ
                     AALTYRYGDEHQPVTTADILTPRRREDYGKDLWSAYQTIQENMLKGGISGRSAKGKRI
                     HTHAIHSIDTDIKLNRALWVMAETLLESLR"
     gene            628..>980
                     /locus_tag="ACHKBL_005249"
     CDS             628..>980
                     /locus_tag="ACHKBL_005249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFO5821947.1"
                     /translation="MKTISHNSTTSSVSVTAASGNDQSQLVATPVPDEQRISFWPQHF
                     GLIPQWVTLEPRIFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDGENWVLFNA
                     MNGNRAEMSPEAAGIV"
BASE COUNT          226 a          267 c          269 g          218 t
ORIGIN      
        1 atgctgcgtc tgcggcgggc cggacagata accggtcagc atgtgcctga aattattctg
       61 ctcaactccc atgacggtac ctccagctac cagatgttac ccggatattt tcgtgccatt
      121 tgtaccaatg gcctggtctg cggtcagtcg ctgggagaag tccgggtgcc acaccgggga
      181 aacgtggtgg acagggtcat agaaggcgct tacgaagtgg tgggcgtgtt tgaccggatt
      241 gaggaaaagc gtgatgccat gcagtcgctg gtcctgccgc caccggcacg ccaggcgctg
      301 gcacaggcgg cactgactta ccgttatggt gacgaacatc agcccgtcac caccgccgac
      361 attctgacgc cacgacgccg ggaggattac ggtaaggacc tgtggagtgc ttatcagacc
      421 attcaggaga atatgctgaa aggtggaatt tcaggtcgca gcgctaaagg aaaacgtatc
      481 catacccatg ccattcacag cattgacact gacattaagc tcaaccgcgc attgtgggtg
      541 atggcggaaa cgctgctgga gagcctgcgc tgattcagcc attaccctga aaaccacgca
      601 tttatttgtc ctttaaggag tcatttcatg aaaaccattt cacataattc caccacatct
      661 tctgtttccg taaccgctgc atcagggaat gaccagtctc agctggttgc cactcccgtc
      721 cctgatgaac agcgcatcag cttctggccg cagcattttg gcctcattcc acagtgggtg
      781 accctggagc cccgtatctt cggctggatg gaccgtcttt gcgaagacta ctgcggtggt
      841 atctggaatc tgtacacact gaacaacggt ggcgcattta tggcccccga accagatgac
      901 gatgatggcg aaaactgggt actgttcaat gccatgaacg gtaaccgcgc tgaaatgagc
      961 ccggaagccg ccggcattgt
//
LOCUS       DAZFLD010000575          972 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.578, whole genome shotgun sequence.
ACCESSION   DAZFLD010000575 DAZFLD010000000
VERSION     DAZFLD010000575.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 972)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 972)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..972
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.578"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>972
                     /locus_tag="ACHKBL_005250"
     CDS             <1..>972
                     /locus_tag="ACHKBL_005250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nuclease"
                     /protein_id="HFO5821948.1"
                     /translation="LCHQARNKLSLSPESPAGWFESGCCEDLLNALNKNKLRDYDALI
                     IDEAQALHSSWLETLSCWFENKRIIAFCDETQTFSFEKGTNYADLQRIINPQFSFNLS
                     VVLRCPRAVTDYLIAVSPASYQITSPRNNEPDTLKELVVTDPLSTLLNEILQLKESSV
                     LPDEITVLVPTDLVKNSLQNDMRTLGVNIETVSRFRGMESSVVITLYSESMEDAQLFC
                     AYSRATTAFIAIYDSEKLAWREKNKFLHKLVNRKEVQRAINEAKHNSSSSVLMAGFFR
                     EGELKLSTVKLAWSKHFSSWLVEFDERNSPEEMWIDYLYSYLPYPII"
BASE COUNT          300 a          167 c          189 g          316 t
ORIGIN      
        1 actttgtcat caggctcgaa acaaattatc cctgtccccc gagtctccgg caggctggtt
       61 tgaatcagga tgctgcgaag atttgcttaa tgcattaaat aaaaataaac taagagatta
      121 tgatgcttta atcattgatg aagcacaggc gttacattct tcgtggctgg agactttatc
      181 ttgttggttc gaaaataaaa ggattatcgc attttgtgat gaaactcaaa cattttcctt
      241 tgaaaaagga acaaattacg ctgatcttca gcgaataata aatccccaat tttcttttaa
      301 cttatctgtt gtgttaagat gtcctagagc cgttactgat tatttaatag ctgtcagccc
      361 tgcctcatac caaattactt cccctaggaa taacgaaccg gatacgctaa aagaattagt
      421 tgttacagat ccactctcca cattgcttaa tgaaatttta caattgaaag aaagttctgt
      481 actgcctgat gagattactg tccttgttcc tacggattta gtgaaaaatt ccctccagaa
      541 tgatatgaga actttggggg tgaatattga aacagtatct cgttttcgag gtatggaatc
      601 atctgtcgtg attacattgt attccgaaag catggaggat gcacagttat tttgtgctta
      661 ttcaagagct acaactgctt ttattgcaat ttatgattcg gagaaattag catggagaga
      721 aaaaaataaa tttttgcata aactggttaa cagaaaggag gttcagagag caattaatga
      781 agcaaagcat aattcttcct ccagtgttct tatggccggt tttttccgcg aaggagagtt
      841 aaaacttagt actgtaaaat tagcatggtc gaagcacttt tcttcgtggc ttgttgaatt
      901 tgatgaaaga aattctccag aagaaatgtg gatagattat ttgtattcct atttgcctta
      961 cccgattata ta
//
LOCUS       DAZFLD010000576          971 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.579, whole genome shotgun sequence.
ACCESSION   DAZFLD010000576 DAZFLD010000000
VERSION     DAZFLD010000576.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 971)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 971)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..971
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.579"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..119
                     /locus_tag="ACHKBL_005251"
     CDS             <1..119
                     /locus_tag="ACHKBL_005251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2859 domain-containing protein"
                     /protein_id="HFO5821949.1"
                     /translation="APGVVMVPASGSVLARRLQISHYPVLITDNGLSQVPAP"
     gene            116..709
                     /locus_tag="ACHKBL_005252"
     CDS             116..709
                     /locus_tag="ACHKBL_005252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000982739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HFO5821950.1"
                     /translation="MMPLSATSAALSASPVLTACLVLSLLAVVLGPALWRSRRKKACV
                     RTRRHQRYRATASRVMKRLNELQGDARRLKYLRKISPYVFEELLLLAFEQQGMPVIRN
                     PSYSGDGGLDGQVVINGERWLIQAKRYGRTIRPAHIDAFGELLRRKGCRGFFIHTGRT
                     GNISRARLTLWPDIELISGQRLLQLLNGTLLSNKGRS"
     gene            706..>971
                     /locus_tag="ACHKBL_005253"
     CDS             706..>971
                     /locus_tag="ACHKBL_005253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000606112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5821951.1"
                     /translation="MIMTLYRFVMLLLRLLIVWPSMLAGCLVMLYVWNNLSGGEAGMT
                     QRYATESSRWRDAPEGHVMVQKCSSVISVSGMYVDEQECPRTAIS"
BASE COUNT          200 a          233 c          300 g          238 t
ORIGIN      
        1 tggcaccggg ggtggtgatg gttccggcat ccggttctgt actggcccgc aggttacaga
       61 taagtcatta cccggtgctg ataacggaca acggcctgtc acaggtacct gcaccatgat
      121 gccgctgtct gccacttctg ctgcgctctc tgcttctccg gtactgacag cctgcctggt
      181 tctcagcctg ctggctgttg tcctggggcc ggcactgtgg cgcagccggc gaaaaaaagc
      241 gtgtgtgaga acacgccggc atcagcgtta tcgtgccacg gcctcacggg taatgaagcg
      301 gctgaacgaa ttgcagggtg atgcccggcg gctgaaatat ctgcgcaaaa tcagtccgta
      361 tgtgtttgag gaactgttgc tgctggcttt tgagcaacag gggatgccgg ttatccgtaa
      421 cccctcatac agcggcgatg gtggtcttga tggtcaggtg gtgattaacg gtgaacgctg
      481 gctgatacag gctaaacgtt acggaagaac cattcgtccg gcacatattg atgcttttgg
      541 cgaactgctg cgccggaagg gatgcagggg cttttttatc catacggggc gtacggggaa
      601 catcagccgg gcacggctga cactgtggcc ggatattgag ctgatcagtg gacaacgtct
      661 gttgcaactg ctgaatggca ccttactgag taataaagga agatcatgat tatgacactg
      721 taccggtttg ttatgctgct gctccggctt ctgattgtct ggccgtcaat gctggcagga
      781 tgtctggtaa tgctgtatgt ctggaacaat ctgagtggtg gtgaggcagg catgacacag
      841 cgttatgcga cggagtcatc ccgctggcgt gatgcgcctg aagggcatgt gatggtacaa
      901 aaatgttcct ccgtcatatc cgtctcaggt atgtatgtcg atgagcagga atgtccgcga
      961 acagcgattt c
//
LOCUS       DAZFLD010000577          950 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.580, whole genome shotgun sequence.
ACCESSION   DAZFLD010000577 DAZFLD010000000
VERSION     DAZFLD010000577.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 950)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 950)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..950
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.580"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..546
                     /locus_tag="ACHKBL_005254"
     CDS             <1..546
                     /locus_tag="ACHKBL_005254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2345 domain-containing protein"
                     /protein_id="HFO5821952.1"
                     /translation="IRFVTDKIRELQSAVLLGSAPQGVALTSGEHLQLSSTRNTMINA
                     GQHLDIGAMKNLSVTVEKALGLFVHKDGAKLVANQGDIEIQAQHNTMALFSEKQLTVT
                     SSEDEIIISTPETLTLNGGGSYLRLSKNGIEHGSSGEFIVKTSDYLVPGSGANLPNEA
                     PGFSLTDITQESKISSKSFND"
     gene            562..>950
                     /locus_tag="ACHKBL_005255"
     CDS             562..>950
                     /locus_tag="ACHKBL_005255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5821953.1"
                     /translation="MSVLPKIVWPIPSSRRGSEFKNQEEILSHLGGESTGLYMIGRSG
                     MWHGGIHITEATTPWCALSGKSPLEAIDFPLPFKGEQAIRCMADGEVIAYRICRDYLT
                     LGWESGPLSFSGSFVLVKHYIQPGEKET"
BASE COUNT          249 a          216 c          265 g          220 t
ORIGIN      
        1 attaggtttg taaccgataa aatcagggaa cttcagtctg cggtcctgct gggtagcgcg
       61 ccacaggggg tggccctgac ctcaggtgaa caccttcagc tcagcagtac ccgtaatacc
      121 atgatcaatg caggtcagca tcttgatatc ggtgcgatga aaaatctgtc ggtaacggtg
      181 gaaaaagccc ttggtctgtt tgtgcataaa gacggggcga agctggtagc caatcaggga
      241 gatattgaga ttcaggctca gcacaacacg atggcgctgt tttcagaaaa acagctgacg
      301 gtgaccagta gtgaagacga aattattatc agcaccccgg aaaccctgac gctgaacggt
      361 ggcgggtctt acctgagact gagtaagaac gggatagagc acggctcatc gggtgagttt
      421 attgtgaaga cgtctgacta tctggtgcct ggttctggcg caaatctccc caacgaagca
      481 cctggtttca gcctgacgga tatcacacag gaaagcaaaa tcagcagcaa gtcttttaac
      541 gactgacagg gagtatgaat cgtgtctgta ttacccaaaa tcgtctggcc gatcccctcc
      601 agccgtcggg gaagtgagtt caaaaatcag gaagagatcc tctctcattt gggaggagaa
      661 tctaccgggc tgtacatgat aggccgtagt ggcatgtggc atggtgggat ccatatcacc
      721 gaagccacga caccatggtg tgcgcttagc ggtaagtctc cacttgaagc gatagatttt
      781 ccgcttcctt ttaaaggtga gcaggcaatc cgatgcatgg ctgacggaga agtgattgct
      841 taccgcatct gcagggatta tctgacgcta ggctgggaat caggtccgct aagtttttcg
      901 ggctcatttg ttctggtgaa acactatatc cagccgggtg agaaagaaac
//
LOCUS       DAZFLD010000578          948 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.581, whole genome shotgun sequence.
ACCESSION   DAZFLD010000578 DAZFLD010000000
VERSION     DAZFLD010000578.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..948
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.581"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            36..596
                     /gene="traP"
                     /locus_tag="ACHKBL_005256"
     CDS             36..596
                     /gene="traP"
                     /locus_tag="ACHKBL_005256"
                     /inference="COORDINATES: protein motif:HMM:NF033885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraP"
                     /protein_id="HFO5821954.1"
                     /translation="MRAPSEPPADAGFAVTPPAAESWAEPMPMTETGSGNTMSAPPVT
                     PQADMQQLARDLKAELDRRDEEIRRTLTMLKESITTLGDTIRKDEAWAQETRQLLDET
                     RKQVHALQMQGPLQIPTGNNGQSTKTPPSRLQGMQIMSVEKGMAWIRWQGSAWAVREG
                     DRLGNITITRIEPSSRSVITSAGTLR"
     gene            593..>948
                     /gene="traQ"
                     /locus_tag="ACHKBL_005257"
     CDS             593..>948
                     /gene="traQ"
                     /locus_tag="ACHKBL_005257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraQ"
                     /protein_id="HFO5821955.1"
                     /translation="MMRIDALTAIENFASGIFSAGMEFLFTWGELLGIIGLIGHLMRA
                     RAEGRHSMGPGKFIAGIVICGMLVALPSFINAGGTQMGFRADSFGPIAYVQPTTFGAA
                     AGAANAMLSLVKLAGVG"
BASE COUNT          217 a          279 c          276 g          176 t
ORIGIN      
        1 tcctcatcat cctggccgca gtctggtaca agtttctgcg ggcaccctca gagccccctg
       61 cagatgcagg cttcgctgtc acaccgcccg cagccgaaag ctgggcagag cccatgccca
      121 tgacggagac cgggagcgga aacaccatgt ccgcgcctcc tgtcacccca caggcagaca
      181 tgcagcagct tgcccgtgac ctgaaagcag agctggacag gcgggatgag gaaatccgac
      241 gtaccctgac catgctgaaa gagagcataa caacccttgg cgacaccata cggaaagatg
      301 aggcctgggc acaggaaacc cgtcagttgc ttgatgaaac ccgtaagcag gtgcatgccc
      361 tgcagatgca gggaccactg caaatcccga ccgggaataa cggccagtca acgaaaaccc
      421 cgccttcccg cctgcagggc atgcaaatca tgtcggtgga aaaaggaatg gcctggatcc
      481 gctggcaggg tagcgcctgg gcagtacggg aaggtgacag gctggggaat atcaccatca
      541 cccggattga accgtccagc cgctcagtca tcacctctgc cggtacatta cgatgatgag
      601 aattgatgcc ctgacagcca tagagaactt tgccagcggt atattcagtg caggcatgga
      661 gtttctcttc acctggggag agctgctggg gattatcggc ctgattggtc atctgatgcg
      721 tgcccgggct gaagggcgtc acagtatggg accgggaaaa tttatcgccg gtatcgtcat
      781 ctgcggcatg ctggtcgccc tgccatcctt catcaatgcc ggggggaccc agatgggatt
      841 cagggcagat tccttcggcc ctatcgctta tgtacaacca accacctttg gtgccgctgc
      901 cggtgccgcc aatgccatgc tctcactggt aaaactggcc ggagtggg
//
LOCUS       DAZFLD010000579          947 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.582, whole genome shotgun sequence.
ACCESSION   DAZFLD010000579 DAZFLD010000000
VERSION     DAZFLD010000579.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..947
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.582"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..304)
                     /locus_tag="ACHKBL_005258"
     CDS             complement(<1..304)
                     /locus_tag="ACHKBL_005258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001279669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication endonuclease"
                     /protein_id="HFO5821956.1"
                     /translation="MSDHNAQLAYPWNAPLPVIDPETFGRAQPTALRQAIQSYINEDI
                     RTDARLDEETVAFLTTTDEGKRINSHLYHDEERRIRLEKLARHKHENPPTLVSEAMA"
     gene            complement(301..711)
                     /locus_tag="ACHKBL_005259"
     CDS             complement(301..711)
                     /locus_tag="ACHKBL_005259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5821957.1"
                     /translation="MKSVKGLSDIQDFFNWMWAVDPRLASRLNDWHDYYRRACKQAAR
                     HVTTYGDFSFTVDDRYRVDVKGDGFGLFHINGTDSTAISWSSSEMTLVLEMTNHSVAV
                     KGIYTAEDFIGEYQRLLVLYRAAFQAATAGECDA"
     gene            complement(780..>947)
                     /locus_tag="ACHKBL_005260"
     CDS             complement(780..>947)
                     /locus_tag="ACHKBL_005260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="derepression protein"
                     /protein_id="HFO5821958.1"
                     /translation="NRRLHRAASVTFIMQSNALHRLNDGIAAGYYAAVFSYLAEDLAE
                     LQDLINQQHHH"
BASE COUNT          189 a          245 c          275 g          238 t
ORIGIN      
        1 ctgccatggc ttcagatacc agtgttggcg gattctcgtg tttgtgccgc gccagttttt
       61 ccagcctgat ccgacgctct tcgtcgtggt aaagatgcga attaattcgc ttcccttcgt
      121 ctgtggttgt caggaaggcg acggtttctt catccagtct ggcgtcagtg cgaatatcct
      181 cgttgatgta gctctgaatg gcctggcgta atgcggttgg ctgcgctctg ccgaacgttt
      241 caggatcgat aaccggtagc ggggcgttcc acgggtacgc cagttgtgca ttatggtcgc
      301 tcatgcgtcg cactccccgg cagtcgccgc ctgaaacgcc gcgcggtaca gcacgagaag
      361 gcgctggtat tcaccgataa agtcttctgc ggtgtatata cctttcacgg ccacgctgtg
      421 gtttgtcatt tccagaacaa gcgtcatctc tgacgacgac caactgatgg cggtgctgtc
      481 ggttccgttg atgtgaaata aaccaaaacc atcacctttc acgtccacgc ggtaacggtc
      541 gtcaacggtg aaggagaaat ccccgtatgt ggtgacgtgt ctggcggcct gtttacaggc
      601 gcggcggtaa tagtcgtgcc agtcgttcag gcgggaagcc agccggggat ccactgccca
      661 catccagtta aaaaaatcct gaatatctga aagtccctta acgctcttca tgagaacctc
      721 cggtaacaga cgtgcggaag cctcccgcgc acgggtgcgg gatggcttca ggatgttgtt
      781 tagtggtggt gttgctggtt gataaggtcc tgcagttctg ccaggtcttc ggccagatag
      841 ctgaagactg ccgcgtaata cccggcagcg ataccatcgt taagtcgatg taatgcgttt
      901 gactgcatga tgaaggtgac agatgcagcc cgatggagtc tgcggtt
//
LOCUS       DAZFLD010000580          940 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.583, whole genome shotgun sequence.
ACCESSION   DAZFLD010000580 DAZFLD010000000
VERSION     DAZFLD010000580.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..940
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.583"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..868
                     /locus_tag="ACHKBL_005261"
     CDS             <1..868
                     /locus_tag="ACHKBL_005261"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5821959.1"
                     /translation="ALAKENGIDVKSDVGKSLFFLCVGKNKGWRKVQKASDYGVIILT
                     EEQFINFIDTGEIPLEQDCVVENDISKSLEKDVNTYKSMIDEFSLSIRTVREFRRSNA
                     LIAVFVDGYAAGWRFAVKECYKDSLDIKLTPVKTEFFNYEIWTQGDSFQFNRGDVFYS
                     DRLAYDSYCDFLKLDDAVVLQVKYECFSGYDSVSTFDGVMDGEFSFSRMEPRPVHKLP
                     VLIETQLYDPGKIIVDVFVPDAAKKQLKLKDTLELKQDEFVCLLQSGCCWVKRREAGD
                     KKKPEFINLFKK"
BASE COUNT          273 a          137 c          222 g          308 t
ORIGIN      
        1 tgctcttgca aaagaaaatg gtattgatgt taagtctgat gttggaaaaa gtttgttttt
       61 cctttgtgta ggaaagaaca aagggtggcg gaaagttcag aaagccagcg attatggcgt
      121 aattattctg acagaagaac aatttatcaa ttttattgat actggtgaaa ttcctcttga
      181 gcaagactgc gtggttgaga atgatatcag taaatcgctt gagaaggatg ttaacactta
      241 caagagtatg atagatgagt tctcgctttc tatccgtact gtaagggagt ttcgccgtag
      301 taatgcgttg attgcggtat ttgttgatgg ttacgcagct ggttggcgct ttgctgtaaa
      361 agagtgttac aaggattctt tggatataaa acttacccca gtaaaaacag agttttttaa
      421 ttatgaaatt tggacgcaag gggattcctt tcagtttaat cgcggtgatg ttttttatag
      481 tgatcgcctg gcgtatgatt cttattgcga ttttttaaag cttgatgatg ctgttgttct
      541 tcaggtgaaa tatgagtgct tttctggtta cgattctgta agtacttttg atggtgtgat
      601 ggatggtgag ttttcttttt ctcgtatgga acccagacct gtccataaat tacctgtgct
      661 gattgaaact caactgtatg accccggaaa gattattgtt gatgtttttg ttcctgatgc
      721 tgcgaaaaaa caattaaaac ttaaagacac cttagagctg aagcaggatg aatttgtctg
      781 tttacttcag tcagggtgtt gttgggttaa acgaagggaa gcaggagata agaaaaaacc
      841 tgaatttatt aacctattca aaaaataaaa atatagcccg catatcgcgg gcaaatgtct
      901 atgtaattca acggagaaag ggtgccatat cgaccgaacc
//
LOCUS       DAZFLD010000581          939 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.584, whole genome shotgun sequence.
ACCESSION   DAZFLD010000581 DAZFLD010000000
VERSION     DAZFLD010000581.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..939
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.584"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
BASE COUNT          288 a          226 c          156 g          269 t
ORIGIN      
        1 ttgtatgatt gtctatgaaa gtacgtgtat atagtatcat accgacattc tcaactcttc
       61 caaacatcca tgcagactga atgttggttt cagtttttca cggccagcat ggggattttt
      121 gttctgaaaa cggagcagat ttactctgga attattttcc ctgtcgggat atttttcacc
      181 tgaaaacttt ctacaacaca acggctgtat aacatcattc tggcatattc attcaggtga
      241 gtcggacaag cgataatcct gttctttacc gaacgaacag gcagcgataa aaaactgatt
      301 cagacgacat ttctgtctga caaacattca catgcagaag accgggacat ttattcttcc
      361 cgaaaggaag tgataccttt tcttatcatg gtagctgcat tacctgatat ctttcaggcg
      421 aatcccctcc ctgtaaatct gatcgtcaga taaaagagat gaacatcagg cagatttccg
      481 tcaataacga taagagatga ccatcgtaaa catatcttcc agtaacgaac tggcacctgc
      541 atattaccgt ttattctcat acatcattga aatattgtca gttataaagg acacacatca
      601 ggaaattatc accatgtgac aacgtcacag atggtgctga tatataaaaa ttaacaaaat
      661 cgcgccaaaa aaccgcgccg atattgcgca tctgtaatcc atcatttcct ttttgaataa
      721 ccttccgtgg ttacccgaca tcctgataat gacgcccatc gtacacgctt ttctttgctc
      781 agacatcgca tacttttatc ctgctcaata ccgtcttcct gccagtcttc ccactctctg
      841 acactcacag aacagcaacg tcctccggac atactagcag ggcagaacac aatccacaac
      901 attaagtata tgtaacaata atataccatt aagaccact
//
LOCUS       DAZFLD010000582          934 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.585, whole genome shotgun sequence.
ACCESSION   DAZFLD010000582 DAZFLD010000000
VERSION     DAZFLD010000582.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 934)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..934
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.585"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            172..858
                     /locus_tag="ACHKBL_005262"
     CDS             172..858
                     /locus_tag="ACHKBL_005262"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HFO5821960.1"
                     /translation="MRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAHLEPAT
                     RWVAHGSGDIIDNIKVFPTLAESLHDVDFTVATTARSRAKYHYYATPVELVLLLEEKS
                     SWMSHAALVFGREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVMVYCYQLATLIQ
                     QPAKSDTTADKHQLQALRERAMALLTTLAVADDIKLVDWLQQRLGLLEQRDTAMLHRL
                     LHDIEKNITK"
BASE COUNT          255 a          197 c          240 g          242 t
ORIGIN      
        1 gcatttttta acaattcaac cgttagtaca gtcaggaaat agtttagctt tttttaagct
       61 aagtaaaggg ctttttctgc gacttacgtt aagaatttgt aaattcgcac cgcgtaataa
      121 gttgacagtg gtcacccggt tcgcggttat ttgagcaaga agagtggcaa tatgcgtata
      181 acgattattc tggtcgcacc cgccagagca gaaaatattg gggcagcggc gcgggcaatg
      241 aaaacgatgg ggtttagcga gctgcggatt gtcgatagtc aggcacacct ggagccagcc
      301 acccgctggg tcgcacatgg atctggtgat attattgata atattaaagt tttcccgaca
      361 ctggctgaat cgttacacga tgtcgatttc actgttgcca ccactgcgcg cagtcgggcg
      421 aaatatcatt actacgctac gccagtagaa ctggtgctgc tgttagagga aaaatcttca
      481 tggatgagcc atgccgcgct ggtgtttggt cgcgaagatt ccgggttgac taacgaagag
      541 ttagcgttgg ctgacgttct tactggtgtg ccgatggtgg cggattatcc ttcgctcaat
      601 ctggggcagg cggtgatggt ctattgctat caattagcaa cattaataca acaaccggcg
      661 aaaagtgaca caacggcaga caaacatcaa ctgcaagctt tacgcgaacg agccatggca
      721 ctgctgacga ctctggcagt ggcggatgac ataaaactgg tcgactggtt acaacaacgc
      781 ctggggcttt tagagcaacg agacacggca atgttgcacc gtttgctgca tgatattgaa
      841 aaaaatatca ccaaataaaa aacgccttag taagtatttt tcagcttttc attctgactg
      901 caacgggcaa tatgtctcgg tgtggattaa aaaa
//
LOCUS       DAZFLD010000583          931 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.586, whole genome shotgun sequence.
ACCESSION   DAZFLD010000583 DAZFLD010000000
VERSION     DAZFLD010000583.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..931
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.586"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>931
                     /locus_tag="ACHKBL_005263"
     CDS             <1..>931
                     /locus_tag="ACHKBL_005263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001499016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HFO5821961.1"
                     /translation="VPGCTIELKARRTTETVIVTTVAYGQPGETGSYSMDVEPGLYRV
                     TLNTEGYAPSYVGDILVKADSAPGTLNKFLMDLEDAQYYPKALAELEAVAAEILKRAE
                     ASAASAEEAKKRAENARGPKGDKGDTGPQGIPGPKGDTGERGPKGERGETGPQGLQGV
                     KGERGEKGEKGEPGGPDATTAQKGIVQLSSATDSDDETKAATPKAVKAAMDKADGCLE
                     KAKNGDDIPDKVKFLNTVGAARVYGRDIHTGAGEWKTREFVAWLKEKGAFDQPYWMMK
                     ASLPAVLNKVITDVGPGKLNLGGCVIEVMGTYEA"
BASE COUNT          259 a          192 c          341 g          139 t
ORIGIN      
        1 cggtaccggg atgcacgata gagctgaaag cgcgacgcac aacggagacg gtgattgtca
       61 ccacggtggc gtatggtcag ccgggggaaa ccggcagtta cagtatggat gttgagccgg
      121 ggttgtaccg ggtgacgctg aacacggaag ggtacgcgcc gtcatatgtg ggtgacattc
      181 tggtgaaggc ggattctgca ccgggaacgc tgaataaatt tctgatggac ctggaagacg
      241 cacagtatta cccgaaagcc cttgcagagc tggaagcggt ggcagcggaa atcctgaaac
      301 gtgcagaagc gtcagcggcc agtgcagagg aagcgaagaa acgggcagag aatgcgcggg
      361 gaccgaaggg ggataaggga gacaccgggc cgcagggtat tcccgggcca aaaggcgata
      421 ccggcgagcg ggggccaaag ggtgagcgtg gtgagacagg accacagggg cttcagggtg
      481 tgaaaggtga acggggagag aagggcgaaa aaggcgagcc gggaggaccg gatgcgacga
      541 cggcacagaa gggaattgtg cagttaagca gcgcaacgga cagtgatgat gaaacgaagg
      601 cagccacccc gaaagcggtg aaagcggcaa tggacaaagc ggacggatgc ctggagaaag
      661 cgaaaaacgg tgacgatatc ccggataagg tgaagtttct gaacaccgtg ggagcagcca
      721 gagtatacgg gcgggacatt catacggggg caggtgaatg gaaaacacgc gagtttgttg
      781 cctggctgaa agaaaaaggg gcatttgacc agccttactg gatgatgaag gcatcactgc
      841 ctgcggtact caataaagtc atcacggatg tcggaccggg aaaactcaat ctgggtggct
      901 gtgtcattga ggtgatggga acgtatgagg c
//
LOCUS       DAZFLD010000584          928 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.587, whole genome shotgun sequence.
ACCESSION   DAZFLD010000584 DAZFLD010000000
VERSION     DAZFLD010000584.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 928)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 928)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..928
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.587"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(51..>928)
                     /locus_tag="ACHKBL_005264"
     CDS             complement(51..>928)
                     /locus_tag="ACHKBL_005264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO5821962.1"
                     /translation="YLLLMAGKVFAFSCNVDGGSSIGAGTTSVYVNLDPVIQPGQNLV
                     VDLSQHISCWNDYGGWYDTDHINLVQGSAFAGSLQSYKGSLYWNNVTYPFPLTTNTNV
                     LDIGDKTPMPLPLKLYITPVGVAGGVVIKAGEVIARIHMYKIATLGSGNPRNFTWNII
                     SNNSVVMPTGGCTVDSRNVNVNLPDFPGSAEIPLGVYCSSEQKLSFYLSGATTDSARQ
                     VFANTAPDATKASGVGVSLMRNGKILATGENVSLGTVNKSKVPLGLSATYGQTGNKVS
                     AGTVQSVIGVTFIYE"
BASE COUNT          255 a          214 c          195 g          264 t
ORIGIN      
        1 tccgcgcaat tttcgcgaat ggagcgcata gactaaggtc aaaatcacaa ttattcatag
       61 ataaaagtga caccaatgac tgactgaact gttccggctg aaactttgtt tcctgtttgt
      121 ccataggttg cgcttaaacc cagaggtact ttgcttttat ttactgtccc cagggagacg
      181 ttttctcccg tagccagtat ttttccatta cgcatcagag agacacccac tcccgatgct
      241 tttgttgcat ctggtgctgt gttagcaaac acttgtctgg cactgtcagt ggtagcaccc
      301 gaaagataaa atgagagctt ttgttcactt gagcaatata cgccaagggg tatttctgcg
      361 cttccaggaa agtccgggag attcacgttg acattacgac tatcaacggt gcagccgcct
      421 gttggcataa caacactgtt atttgaaata atattccacg taaaattacg cggatttccg
      481 cttcctaacg tggcaatttt atacatatga atacgggcaa taacttcccc tgcttttatg
      541 acaactccac cggccactcc gactggggta atatagagtt ttaacggtaa tggcatcggt
      601 gtcttatcac caatatccaa tacgttggta tttgtcgtca acgggaaggg gtaggtcaca
      661 ttattccagt acagtgatcc tttatatgac tgtagggagc ctgcgaaagc tgatccttgt
      721 accaggttta tatgatcagt gtcgtaccag ccgccgtaat cattccagca acttatatgc
      781 tgagacaagt ctacaaccag attctggccc ggttgtatta caggatcgag attaacataa
      841 acagaggttg tacctgctcc aatacttgac ccgccatcga cattacaact gaaggcaaaa
      901 actttgcctg ccattaatag aagataaa
//
LOCUS       DAZFLD010000585          927 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.588, whole genome shotgun sequence.
ACCESSION   DAZFLD010000585 DAZFLD010000000
VERSION     DAZFLD010000585.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 927)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..927
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.588"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            21..233
                     /locus_tag="ACHKBL_005265"
     CDS             21..233
                     /locus_tag="ACHKBL_005265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001379414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFO5821963.1"
                     /translation="MSEDVPLPKANQRYRDDHGALVTVTSVEETRVVFMRDGYPHPCM
                     RPMYNFLGKFKPEPREEKIPAGNHTA"
     gene            complement(253..486)
                     /locus_tag="ACHKBL_005266"
     CDS             complement(253..486)
                     /locus_tag="ACHKBL_005266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5821964.1"
                     /translation="MARSDYDIINLSPEHELNEWLAERGYAGLADNRNRLAEVVACKL
                     QDSFCMNVSWDALNAAYSEHPEWFSGLVSGDEN"
     gene            complement(582..>927)
                     /locus_tag="ACHKBL_005267"
     CDS             complement(582..>927)
                     /locus_tag="ACHKBL_005267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFO5821965.1"
                     /translation="FGCVYSLKASLSGVPDTYRYHLSHRIRRMVGRENTSSPYQQIAR
                     EVKAPRERLKYALEAGLLVTALDGLFWSGSQRIAADVLRLRQSGMPVVTTSVEVSDNL
                     TGTTRKIPAYHL"
BASE COUNT          219 a          268 c          220 g          220 t
ORIGIN      
        1 ctgctaccgt tctgaaatct atgtctgaag atgttccatt accgaaagcc aatcagcgat
       61 acagagacga tcatggtgcg cttgtcaccg tgaccagtgt tgaggaaacc cgggtggtat
      121 ttatgcggga cggctatccg catccctgca tgcggccgat gtacaacttc ctgggaaaat
      181 tcaaacccga accccgggaa gaaaaaatcc ctgccggcaa tcacactgcc tgatggcagc
      241 ataatcctct ttttaattct catccccgga gacaagccct gaaaaccact cgggatgttc
      301 actgtatgct gcgttcagag catcccagga aacgttcata cagaagctgt cctgcaattt
      361 gcaggcaacc acctctgcca ggcggttccg gttatccgcc agtccggcat aacctctctc
      421 tgccagccac tcattcagtt catgttccgg agacagattg ataatatcgt aatcggacct
      481 tgctattccc gtgtccttag cacatgtttt caggcacggg gataaagtat aaagaatgtc
      541 gtactgctgc tgccagagca atattccccc tgatggtcgt atcagagatg gtatgccggt
      601 attttgcggg ttgttcccgt caggttatcg ctcacttcca cggacgttgt caccaccggc
      661 attccgctct gtcttagtct caggacatca gctgcaatac gctgactgcc agaccagaac
      721 aggccgtcca gtgcagtcac cagtaacccc gcctccagcg catacttcag ccgttcacgg
      781 ggcgctttca cttcccgggc aatctgctgg tatggcgatg atgtattttc acgcccaacc
      841 atccggcgaa tacgatgaga cagatgatac cggtatgtat ccggcacacc ggaaaggctg
      901 gccttcaggc tgtacacgca gccaaag
//
LOCUS       DAZFLD010000586          927 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.589, whole genome shotgun sequence.
ACCESSION   DAZFLD010000586 DAZFLD010000000
VERSION     DAZFLD010000586.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 927)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..927
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.589"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..272
                     /locus_tag="ACHKBL_005268"
     CDS             <1..272
                     /locus_tag="ACHKBL_005268"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5821966.1"
                     /translation="KTEKRRTGMEEMQDKMAWMQNLITEKLGPSLWHMHPIAFPGALI
                     KKNTGWAHSKFANLLGSVESENDYSAYNVTKTGKSVPHYNINLTK"
     gene            443..>927
                     /locus_tag="ACHKBL_005269"
     CDS             443..>927
                     /locus_tag="ACHKBL_005269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5821967.1"
                     /translation="MGCNKKSNKFLLFVSCMLLSFVTYSQDSSNPQFSDYLVPVSNGP
                     FEKNIHFNKEQENYSQHWKNAVQEELKKPVNFAGHFRIYTAFGGHGKECLRDNWVCGW
                     VIDKLSGEVVATLPSDNNGSYNYADVSDNGTPVGLPFEIDTYKNSSMIAITGQSISVS
                     K"
BASE COUNT          309 a          153 c          207 g          258 t
ORIGIN      
        1 gaaaaactga aaaaagacgt accggaatgg aagaaatgca ggataagatg gcctggatgc
       61 agaatctgat cacggagaaa ctgggccctt cgttgtggca tatgcatccg attgcctttc
      121 ctggagcatt aataaagaaa aatactgggt gggcgcatag caagtttgca aaccttttgg
      181 gaagtgtcga gtcagaaaat gattattcgg catataatgt tactaagaca ggtaaatctg
      241 tcccgcacta taatattaat ctcacaaaat gacattatct gaggtaattg atgccccaag
      301 agaacgaaaa atggtaaaaa gaaatacagg ttagtaccta gggtggcaga agttgagggg
      361 gagtcgtact attctggtga tggattgaat aaagctcacc tattaccaga tacgatgatt
      421 aaagctcttg aggattctaa aaatgggatg caataaaaaa tcaaacaaat ttttgctttt
      481 tgtgtcatgc atgctcttgt cgtttgtaac gtactcacaa gacagttcta atcctcagtt
      541 ttctgactac ctggttcctg taagcaacgg accatttgaa aagaatattc actttaataa
      601 agagcaggaa aattactcgc aacactggaa gaatgcagtc caagaggaac taaaaaagcc
      661 tgttaatttt gcagggcact ttagaatcta tacagccttt ggcggtcatg gtaaagaatg
      721 ccttcgtgat aattgggtat gtggttgggt tattgataaa ctatcagggg aagttgtagc
      781 aacacttcca tcagataata atggaagtta taactatgct gatgtgtctg ataatggtac
      841 ccctgttggt cttccatttg agattgatac ttataaaaat agttcaatga ttgcaataac
      901 cggtcaatct atctcagtaa gcaagag
//
LOCUS       DAZFLD010000587          926 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.590, whole genome shotgun sequence.
ACCESSION   DAZFLD010000587 DAZFLD010000000
VERSION     DAZFLD010000587.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..926
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.590"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..270)
                     /locus_tag="ACHKBL_005270"
     CDS             complement(<1..270)
                     /locus_tag="ACHKBL_005270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFO5821968.1"
                     /translation="MKGLLALLISSMVLPAHAGIVIYGTRIIYPAENKEVMVQLMNQG
                     NRSSLLQAWIDDGDTSLPPEKIQVPFMLTPPVAKIGANSGQQVKIK"
     gene            complement(351..893)
                     /gene="yehD"
                     /locus_tag="ACHKBL_005271"
     CDS             complement(351..893)
                     /gene="yehD"
                     /locus_tag="ACHKBL_005271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO5821969.1"
                     /translation="MKRSIIAAAVFSSFFMSAGVFAADVDTGTLTIKGNIAESPCKFE
                     AGGDSVSINMPTVPTTVFEGKAKYSTYDDAVGVTSSMLKISCPKEVAGVKLSLITNDK
                     ITGNDKAIASSNDTVGYYLYLGDNSDVLDISAPFNIESYKTAEGQYAIPFKAKYLKLT
                     DNSVQSGDVLSSLVMRVAQD"
BASE COUNT          265 a          202 c          175 g          284 t
ORIGIN      
        1 tttaattttt acttgctgcc cggaatttgc ccctattttt gccactggtg gcgttaacat
       61 gaaaggaacc tgaatttttt ctggtggtaa tgacgtatcg ccatcatcaa tccacgcctg
      121 cagcagcgaa gaacggtttc cctggttcat caactgcacc atcacttctt tattttctgc
      181 cgggtaaata atgcgcgtcc cgtagataac aataccggca tgtgcaggaa ggaccataga
      241 agaaatgagt aaagctaata atcctttcat cttaatgcct cttaaattaa aaggccgcca
      301 tggggatagc ggccatgttt attacctgga gaaacttgca attttattta ttaatcctgc
      361 gccacacgca taaccagaga agataacaca tcacctgatt gcactgaatt atctgtcagc
      421 ttcaggtatt ttgctttaaa cggaatagca tattgacctt ccgctgtttt ataactctca
      481 atgttaaaag gtgcagaaat atccaggaca tcactgttat cacctaaata gagatagtaa
      541 cccacggtat cgttgctact ggctatcgcc ttatcgttgc cggttatttt gtcgtttgtg
      601 atcaacgaga gttttacacc agcaacttct ttcgggcagc taatttttaa catgctgctg
      661 gttacaccga ctgcatcatc ataggtagaa tatttagctt taccttcaaa gacagtggtt
      721 ggtacagttg gcatattaat acttactgaa tcgccacctg cttcgaattt acacggagat
      781 tctgcaatat tccctttaat ggttaatgtt ccggtatcaa cgtctgcggc aaatacccca
      841 gcgctcataa aaaaagaaga aaaaacagcg gcagcaataa ttgaacgttt cataatatgc
      901 tccttaataa aatctatttt tggttg
//
LOCUS       DAZFLD010000588          923 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.591, whole genome shotgun sequence.
ACCESSION   DAZFLD010000588 DAZFLD010000000
VERSION     DAZFLD010000588.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..923
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.591"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..673
                     /locus_tag="ACHKBL_005272"
     CDS             <1..673
                     /locus_tag="ACHKBL_005272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LexA family protein"
                     /protein_id="HFO5821970.1"
                     /translation="RLRKIWDEKRKQLSLSQEKAADILGFKTQGAVSQLLNAKIALNT
                     ENTLKFAALLQVPAEEINPDLSDLLRSIRTHTPGNRRNLFEKYYEYPLLSCVQAGAFS
                     MDDFSYTAKDAIKWISTTTKASDRSFWLEVKGHSMTAPQGGKPSFPEGMLILVDPERE
                     IEDGDFCVARMNGDEFTFKRFIRESGKAYLEPLNPRFDMIECNENCQFVGKVIKSQWN
                     DETFD"
     gene            754..>923
                     /locus_tag="ACHKBL_005273"
     CDS             754..>923
                     /locus_tag="ACHKBL_005273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system HicB family antitoxin"
                     /protein_id="HFO5821971.1"
                     /translation="MKYLKYKGYLGTVVTDFENNVLYGKLAFIRDLVTYEAKTLADLE
                     QEFKTSVDLYLHS"
BASE COUNT          285 a          186 c          221 g          231 t
ORIGIN      
        1 acgactgcgc aaaatctggg atgaaaaacg gaaacagcta tccctaagcc aggaaaaagc
       61 cgcagatatc ctcggcttca aaacgcaggg ggctgttagt cagttattga acgcaaaaat
      121 tgcgttaaat actgaaaata cccttaagtt tgcggcttta ttacaggttc ctgcggaaga
      181 aattaatcca gatctgagtg atttgttacg cagtattcgt acccacacac cgggaaacag
      241 gcgtaacctg ttcgagaaat attacgaata tccgttactc tcctgcgtac aagctggagc
      301 tttctcaatg gatgattttt cgtacaccgc aaaggatgcg attaagtgga tctccaccac
      361 cacaaaagcc agtgacagat cgttctggct ggaggtcaag gggcattcaa tgaccgcgcc
      421 acagggaggt aaacccagct tccctgaagg aatgctgata cttgttgacc ctgaacggga
      481 aatcgaggat ggcgattttt gcgtggcccg aatgaacggc gatgaattca ccttcaaacg
      541 attcattcgt gagagcggta aagcgtacct ggagccactc aacccacgat tcgacatgat
      601 tgagtgtaac gaaaactgcc agtttgtagg aaaagtcata aagtcgcagt ggaatgatga
      661 gacttttgat tgaattcaca gaacaattta acatccatca cgtcctttgg ggcgtggagg
      721 atgttaaaca aaacgtgaag aggctggatt attatgaaat atttaaaata taaaggatat
      781 ttaggtacag ttgtcacaga ttttgaaaat aatgtcctgt atggaaagct ggcgtttatc
      841 cgtgatctgg tgacttatga ggcaaaaaca ttagcagacc tggaacagga gtttaaaaca
      901 tcggttgatc tgtacttgca ctc
//
LOCUS       DAZFLD010000589          913 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.592, whole genome shotgun sequence.
ACCESSION   DAZFLD010000589 DAZFLD010000000
VERSION     DAZFLD010000589.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 913)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..913
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.592"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            5..829
                     /gene="gadX"
                     /locus_tag="ACHKBL_005274"
     CDS             5..829
                     /gene="gadX"
                     /locus_tag="ACHKBL_005274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadX"
                     /protein_id="HFO5821972.1"
                     /translation="MQPLHGNCLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIQV
                     DKCVENFVLVSRDTLSLFLPMLKEEALKLHVHKKVPSLLVHHCTRDIPVFQEVAQLSQ
                     NKNLRYAEMLRKRALIFALLSVFLEDTQFIPLLLNVLQPNMRTRVCTVINNNIAHEWT
                     LARIASELLMSPSLLKKKLREEGTSYSQLLTECRMQRALQLIVIYGVSIKRVAVSCGY
                     HSVSYFIYVFRNYYGMTPTEYQERSAQELPNCGSAASMAAQGIFYGTDRSAEGIRL"
BASE COUNT          264 a          169 c          208 g          272 t
ORIGIN      
        1 aactatgcaa ccactacatg gtaattgtct aattgcgtac gcaagacata aatatattct
       61 caccatggtt aatggtgaat atcgctattt taatggcggt gatctggttt ttgcggatgc
      121 aagccaaatt caagtggata agtgtgttga gaatttcgta ttagtttcaa gagacacgct
      181 ttcattattt ctcccgatgc tcaaggagga ggcattaaaa cttcatgtac ataaaaaagt
      241 tccttcatta ctcgttcatc actgtaccag agatattcct gtttttcagg aagttgcgca
      301 actatcgcag aataagaatc ttcgctatgc agaaatgcta cgtaaaaggg cattaatctt
      361 tgcgttgtta tctgtttttc ttgaggatac gcagtttata ccgttacttc tgaacgtgtt
      421 gcaaccgaat atgcgaacac gagtttgtac ggtcatcaat aataatatcg cccatgagtg
      481 gacattagcc cgaattgcta gcgaattgtt gatgagtcca agcctgttaa agaaaaaatt
      541 gcgcgaagaa ggtacgtcat attcacagtt gcttactgag tgtaggatgc agcgtgcttt
      601 acaacttatt gttatatatg gggtttcaat taagcgagtt gcagtatcct gtggatatca
      661 cagcgtgtcg tatttcattt acgtttttcg aaattattat gggatgacgc ccacagagta
      721 tcaggagcga tcggcgcagg aattaccgaa ctgtggctcg gcggcaagta tggctgcgca
      781 agggattttt tacggcactg accgttctgc ggaaggaata agattataga gttttactca
      841 gacataaaaa aaacccggca taggggaccg ggaagaggat agtctgccgt ctccagacta
      901 ataaaccgtt ata
//
LOCUS       DAZFLD010000590          909 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.593, whole genome shotgun sequence.
ACCESSION   DAZFLD010000590 DAZFLD010000000
VERSION     DAZFLD010000590.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 909)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 909)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..909
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.593"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>909
                     /locus_tag="ACHKBL_005275"
     CDS             <1..>909
                     /locus_tag="ACHKBL_005275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001622451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFO5821973.1"
                     /translation="KGIYLNVAWNYSKSPGTNADKIVSLNVSLPISNWLSSTNDGRSS
                     SNAMTATYGYSQDNHGQVNQYTGVSGSLLEQHNLSYNIQHGFANQDNSSSGSVGVNYR
                     GAYGSLNSAYSYDNEGNQQINYGISGALVVHENGLTLSQPLGETNVLIKAPGANNVDV
                     QRGTGISTDWRGYAVVPYATEYRRNNISLDPMSMNMHTELDITSTEVIPGKGALVRAE
                     FAAHIGIRGLFTVRYRNKSVPFGATASAQIKNSSQITGIVGDNGQLYLSGLPLEGVIN
                     IQWGDGVQQKCQANYKLPETELNNPV"
BASE COUNT          269 a          160 c          210 g          270 t
ORIGIN      
        1 taagggtatc tatctaaatg ttgcgtggaa ttacagtaaa tcaccaggga caaatgcgga
       61 taaaattgtc tcgctaaatg tctcattacc tataagtaat tggttatctt ccacgaatga
      121 tgggcgctca tcatcgaatg ccatgactgc aacgtatggt tatagtcagg ataaccacgg
      181 acaggtaaac caatatacgg gggtatctgg ttctctgttg gagcagcata atctcagtta
      241 taacatacaa catggttttg ctaatcagga taatagcagt agtggttctg ttggtgttaa
      301 ttatcgtggg gcatatggtt ccttgaattc cgcctacagt tacgataatg aaggtaatca
      361 acagataaac tatggcatca gtggtgctct tgttgtacat gaaaatggtc ttacgttgag
      421 tcaaccatta ggtgaaacta atgttttgat aaaagcgcct ggagcgaata atgtggatgt
      481 ccagcggggg acaggaatat ccactgactg gcgtggatat gcagttgttc cttatgcaac
      541 agaatataga cgtaataata tttcattaga tcctatgtca atgaatatgc atactgaact
      601 ggatatcact tccactgaag ttattccggg aaaaggtgcg ttagttcgtg cagagtttgc
      661 tgctcatatc ggtattcgtg gtttgttcac agttcgttat cgtaataaat cagtcccatt
      721 cggtgctaca gccagcgcac agattaaaaa tagtagtcaa attaccggga ttgtcggcga
      781 taatggacaa ctttatctct caggattgcc tttagaaggt gttattaata tccagtgggg
      841 agatggtgtt cagcaaaaat gtcaggctaa ttacaagctc cctgaaacag aactgaataa
      901 tcctgttag
//
LOCUS       DAZFLD010000591          904 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.594, whole genome shotgun sequence.
ACCESSION   DAZFLD010000591 DAZFLD010000000
VERSION     DAZFLD010000591.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..904
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.594"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..375
                     /locus_tag="ACHKBL_005276"
     CDS             <1..375
                     /locus_tag="ACHKBL_005276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001534332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO5821974.1"
                     /translation="QDGIKSHTHGGSVSNTNLGTKTTSSFDYGTKTTSTNGQHEHSVP
                     LRRQDGGGINFDWLDGSSSGSHIGNGYVPAAGNHQHTIPIGAHTHTIALGSHGHSLTI
                     NPTGNAENTVKNIAFNYIVRLA"
     gene            375..>904
                     /locus_tag="ACHKBL_005277"
     CDS             375..>904
                     /locus_tag="ACHKBL_005277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFO5821975.1"
                     /translation="MTFKMSEHPQTIKIYNLSAGTNEFIGEGDAWIPPHTGLPAHSTD
                     IAPPDIPEGHVAIFDEDENTWHLMEDHRGKTVWRLESGSALLITEPGPLPENVTIIKP
                     DGEFCRWDGSSWAKDEAAEQAYRVSEAENKKRELIQEATNTINILQDAVDLNMATDDE
                     SALLPLWKKYRVLLSRT"
BASE COUNT          273 a          242 c          218 g          171 t
ORIGIN      
        1 caggacggca tcaaatccca cacccacggg gggagcgtct caaacactaa ccttggcacg
       61 aaaacgacct catcatttga ttacggaacc aaaacaacct ctacgaacgg acaacatgaa
      121 cacagcgtcc ccctgagaag acaagatggc ggaggcataa actttgactg gctggatggt
      181 tcatcgtctg gttctcatat tggtaatgga tacgttcctg ccgcaggtaa ccaccaacat
      241 accataccca ttggcgcaca cacccacacg atcgcactgg gttcacatgg tcacagcctg
      301 accattaacc ccaccggtaa tgccgaaaac accgttaaaa acatcgcatt taactacatc
      361 gtgaggcttg cataatgacc tttaaaatga gcgaacaccc gcagacaatt aaaatctaca
      421 acctgtcagc cggaacaaat gaatttatcg gcgaaggcga tgcatggatc cctccgcata
      481 ccggactgcc tgcgcactca acggacattg cccctcctga cattcctgaa ggacacgtgg
      541 ctatctttga tgaggatgaa aacacctggc acctcatgga agatcaccgg ggtaaaaccg
      601 tctggcgtct ggaatcggga agcgccttac tgattactga gccgggcccg ttaccggaaa
      661 acgtgaccat aataaaaccg gacggggagt tctgccgctg ggacgggtca tcatgggcta
      721 aggatgaagc ggcagaacag gcttaccggg tgagcgaggc ggagaataaa aaacgggaat
      781 taatacagga agcaacaaat acaataaaca tccttcagga tgccgtggat ttaaatatgg
      841 cgaccgacga tgaaagcgcc ctgcttcccc tgtggaaaaa ataccgggta ttactgagcc
      901 ggac
//
LOCUS       DAZFLD010000592          902 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.595, whole genome shotgun sequence.
ACCESSION   DAZFLD010000592 DAZFLD010000000
VERSION     DAZFLD010000592.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..902
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.595"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>902)
                     /locus_tag="ACHKBL_005278"
     CDS             complement(<1..>902)
                     /locus_tag="ACHKBL_005278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFO5821976.1"
                     /translation="KNKNTYSNLDIEPVASSGVVHTGSNPVMVKVGRTKKLMFKDGSG
                     AFRIHLGKNEVVHIVRYIHNSSIRMEYAIGTTKGLFMLHNLCEAGRVKLNRSLPRKGI
                     WKIGDYQGIYYYGKSRKEEIENALRFSGHPKFSELENDFNQFFSDIDYYTRYGQSGMR
                     KILFTGPPGTGKTTIAKALGAKYQDKYVFVYADNYFREVCYAAANKKIPLIIIAEEAD
                     ELYRADAGTLSFLDGADTPRNPAGTYVIFSTNYPRRIDPRIRKRPGRIDRVISIGAFR
                     TKDAASCAKMYLPEGININIKELG"
BASE COUNT          269 a          202 c          134 g          297 t
ORIGIN      
        1 cgccaagctc tttaatattg atgtttatac cttctggtaa atacatcttt gcacaactag
       61 cagcatcttt agtcctgaaa gcaccaatag aaataactct gtcaattctt cccggacgtt
      121 tcctgatacg aggatcaatt ctcctaggat agtttgttga gaatattaca tatgtcccgg
      181 caggatttct cggcgtatca gccccatcca gaaaacttag cgtaccagca tcagctctat
      241 aaagctcatc agcctcttca gctataataa taagaggtat tttcttgttc gctgcggcat
      301 aacaaacttc tctaaaatag ttatcagcat atacaaatac atacttatcc tgatactttg
      361 ccccaagagc tttggctatt gtcgttttac ctgttcctgg tgggcctgta aacagaatct
      421 tcctcattcc agactgacca taacgcgtat agtaatcaat atcagaaaaa aactgattaa
      481 agtcgttctc cagttcagaa aatttaggat gacctgaaaa acgtaaggca ttttcaatct
      541 cttcttttct agatttaccg taataataaa tcccctgata atcacctatt ttccagatac
      601 cttttcttgg taacgaacga tttagtttaa cgcgccccgc ttcacaaaga ttatggagca
      661 taaacaaacc tttagttgtt cctatagcat attccattct aatactggaa ttatgaatat
      721 atctgacaat atgcacgact tcatttttcc ccaaatgaat tctgaatgct cctgacccgt
      781 ctttaaacat gagtttttta gtccttccca ctttaaccat tactggatta cttcctgtat
      841 gaacaactcc agaagaagct acaggttcga tatcaagatt tgaatatgta tttttatttt
      901 tc
//
LOCUS       DAZFLD010000593          901 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.596, whole genome shotgun sequence.
ACCESSION   DAZFLD010000593 DAZFLD010000000
VERSION     DAZFLD010000593.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..901
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.596"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..587
                     /locus_tag="ACHKBL_005279"
     CDS             <1..587
                     /locus_tag="ACHKBL_005279"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5821977.1"
                     /translation="ITGTVTEVIAMRLTPMGIPGKDPAHVKAWTQQEDRLLISLYQEH
                     NTHQMAERIQRTRKAVMYRIRFLRNRGLIGRKRKAPLSTEAIAFLIKNRHVKTAQEMA
                     LEVGCSECTVRYTLRKRGYNLKKCGENHHCARYSDHLVELVTELRDIHNMTFCMIAEH
                     INSTIQMHINDDAAFHLYHRRTATDAVLCELLPG"
     gene            597..>901
                     /locus_tag="ACHKBL_005280"
     CDS             597..>901
                     /locus_tag="ACHKBL_005280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001326016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5821978.1"
                     /translation="MHTQKNRLPAFAWSGYSFPAPHKTGAGITTPPTTRAHNRASGFF
                     VRTVSPRLFRVRIMAGRTGPTSVGPGSLVAGCGNPVRLATLRLPPRVGEFSELTTRG"
BASE COUNT          240 a          236 c          233 g          192 t
ORIGIN      
        1 gaataaccgg aacagtaacg gaggttatcg cgatgagact tacacctatg ggaataccgg
       61 gcaaagaccc ggcacatgtg aaggcgtgga cacagcagga agatcggtta ctcatctcgc
      121 tttatcagga acacaacaca catcagatgg ctgaacgaat tcagcgaacc cgtaaagcag
      181 ttatgtaccg gattcgtttt ttacgtaatc gtgggctgat tggccgcaag agaaaagcac
      241 cactgagtac agaagccata gcctttctta ttaaaaaccg tcatgtaaaa accgcacagg
      301 aaatggctct ggaagtggga tgcagtgagt gcacagtgag gtacacactg cgtaaacggg
      361 gttacaacct gaaaaaatgc ggtgagaacc accattgcgc cagatacagc gatcatctgg
      421 ttgagctggt cacggagcta cgcgacatac acaacatgac gttctgcatg atcgctgaac
      481 acatcaacag cacgatacag atgcacatta acgacgatgc ggcgtttcat ctctaccacc
      541 gccgcaccgc aactgacgca gtgctctgtg aactgttacc gggctgagtg atcatcatgc
      601 acacacaaaa aaaccgcttg ccagcgttcg cctggtcggg ttacagtttt cccgcacctc
      661 ataaaacggg tgccgggatt accaccccgc cgactacaag agcgcacaac cgcgccagcg
      721 gtttttttgt gcgtactgta tcgccacgtc tttttcgcgt cagaattatg gcggggcgta
      781 cggggccgac ttcggtcggg ccgggatctc ttgtagccgg ttgtggtaac cctgtacgtc
      841 tcgccaccct gagattaccg cctcgggttg gtgagttttc agaacttact acaagaggcc
      901 a
//
LOCUS       DAZFLD010000594          898 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.597, whole genome shotgun sequence.
ACCESSION   DAZFLD010000594 DAZFLD010000000
VERSION     DAZFLD010000594.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 898)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 898)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..898
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.597"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            101..697
                     /locus_tag="ACHKBL_005281"
     CDS             101..697
                     /locus_tag="ACHKBL_005281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HFO5821979.1"
                     /translation="MLWTENDAANTSQWNGYPLQIGRFRKDKAMPALISGEKSAALVT
                     PPQWRNKAFNGLKDPERNYWAKEQITGSPEENIKAAITYLMMKLSNTKEESTIDQYDS
                     TLYSVIVQKGDLADNIRKERKTTIPNLTKNNPGKNLDKIHPGDILYYQKASMKVIITG
                     WKPITIKNVAMNYNGGGDPKYAIKLQFVYTLLTKNRVL"
     gene            700..>898
                     /locus_tag="ACHKBL_005282"
     CDS             700..>898
                     /locus_tag="ACHKBL_005282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5821980.1"
                     /translation="MGKITIILLLTFSCTKCLAFDISMSKECSGVDELEFVDCVKNSF
                     IMSTITLKKAEVNIKEKIKKWN"
BASE COUNT          354 a          149 c          151 g          244 t
ORIGIN      
        1 taaatatgac ttattaattt taaaatctgt taatgaaatc aacattcatt tgtccagcac
       61 tccatacttt cagccgttgg attggtatat aataaaagcc atgctttgga ctgaaaacga
      121 tgcggcaaat acttctcagt ggaatggtta tccattacaa attggtcgtt ttcggaaaga
      181 taaagcgatg cctgctttaa tctctggaga aaaatccgca gcccttgtta ccccaccaca
      241 atggagaaac aaagcattca atgggttaaa agatccagaa agaaattact gggcaaaaga
      301 acaaataact ggctctccag aagagaatat caaagcagca attacttact taatgatgaa
      361 actttctaat acaaaggaag aatcaactat cgatcaatat gactcaactc tatatagcgt
      421 catcgtccaa aaaggcgatt tggctgacaa catcagaaaa gagagaaaaa cgaccatacc
      481 taacttaacg aaaaacaacc ctggaaaaaa tctcgataaa atacacccgg gagatatttt
      541 atattatcaa aaagcatcca tgaaagtaat aattactgga tggaaaccta tcacaataaa
      601 aaatgtagcg atgaattata atggtggtgg agatcccaag tatgcaataa aacttcagtt
      661 cgtttataca ttgctcacaa aaaacagagt gttatgagaa tgggaaaaat aacaattatt
      721 ctactgctta cattctcatg tacaaaatgt ctggcatttg acatttccat gtctaaagaa
      781 tgtagtggcg ttgatgaact tgaatttgtt gattgtgtta aaaattcatt tattatgagc
      841 actattacac ttaaaaaagc agaagtaaat ataaaagaga aaattaaaaa atggaata
//
LOCUS       DAZFLD010000595          896 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.598, whole genome shotgun sequence.
ACCESSION   DAZFLD010000595 DAZFLD010000000
VERSION     DAZFLD010000595.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..896
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.598"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(101..748)
                     /gene="ychE"
                     /locus_tag="ACHKBL_005283"
     CDS             complement(101..748)
                     /gene="ychE"
                     /locus_tag="ACHKBL_005283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YchE"
                     /protein_id="HFO5821981.1"
                     /translation="MIQTLFDFPVYFKFFIGLFALVNPVGIIPVFISMTSYQTAAARN
                     KTNLTANLSVAIILWISLFLGDTILQLFGISIDSFRIAGGILVVTIAMSMISGKLGED
                     KQNKQEKSETAVRESIGVVPLALPLMAGPGAISSTIVWGTRYHSISYLFGFFVAIALF
                     ALCCWGLFRMAPWLVRVLRQTGINVITRIMGLLLMALGIEFIVTGIKGIFPGLLN"
     gene            complement(779..832)
                     /gene="ychE"
                     /locus_tag="ACHKBL_005284"
     CDS             complement(779..832)
                     /gene="ychE"
                     /locus_tag="ACHKBL_005284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YchT"
                     /protein_id="HFO5821982.1"
                     /translation="MLSKGVLSARLFNLIYG"
BASE COUNT          278 a          223 c          186 g          209 t
ORIGIN      
        1 ttttcaccca ttccttttgc ttttgataac tgcgcgggaa caaagtaaaa ataatgacga
       61 agtaaacgga gcatgacagc tccgtttcat ttgaaaggaa ttaattaagc aggccgggga
      121 aaatcccctt aataccagta acgataaatt caatccccaa tgccatcagc aatagcccca
      181 taatacgcgt aatcacgttg atgccggtct ggcgtaaaac ccgtaccagc cacggtgcca
      241 tgcggaacaa tccccagcaa cataaagcga acaatgcaat agccacaaag aaaccaaaca
      301 gatagctaat gctgtgataa cgcgtccccc agacgatggt agaactgatc gcccctggcc
      361 ccgccatcaa cggcaacgcc aatggcacca caccaatgct ttcacgcacc gcggtttctg
      421 atttttcttg cttgttctgt ttatcctcgc caagcttgcc gctgatcatc gacatcgcta
      481 tcgtcaccac caggatgcca ccggcgatac ggaatgaatc aattgatata ccaaaaagtt
      541 gtagaatcgt gtcgccgaga aaaagcgaga tccacaagat aatggccaca gacaggttgg
      601 ctgtaaggtt cgttttgttt cgcgctgccg ctgtctgata actggtcatg ctgataaaga
      661 cgggaataat ccctaccggg ttgaccagcg caaataaccc gatgaaaaat ttgaagtaaa
      721 cgggaaaatc aaaaagggtc tgaatcacgg ttagctccga agcaaaagcc ggataatgtt
      781 agccataaat aaggttaaaa agacgcgctg acaatacgcc ttttgacagc atttttcacc
      841 tcctaactac ttaaaattgc tatcattcgt tattgttatc tggttgtgca aaacat
//
LOCUS       DAZFLD010000596          894 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.599, whole genome shotgun sequence.
ACCESSION   DAZFLD010000596 DAZFLD010000000
VERSION     DAZFLD010000596.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..894
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.599"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            50..850
                     /locus_tag="ACHKBL_005285"
     CDS             50..850
                     /locus_tag="ACHKBL_005285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5821983.1"
                     /translation="MNSKKVCCICVLFSLLAGCASQSSIDEKKKKAQVTQSNINKNIP
                     PQLTDKDLFGNETTLAVSEEDIQAALEGDEFRVPLNSPVILVQSGSRAPETIMQEEMR
                     KYYTVSTFSGIPDRQKPLTCNKNKDKNENEDVASAENMNWMQALRFVAAKGHQKAIIV
                     YHDTLQTGKYDSALKSTVWSDYKNEKLTDAISLRYLVRFTLVDVATGEWATWSPVNYE
                     SRVIFPPAGITKMSNKELSNDHVTEVQLFNLKQKTYVSMVKDLVSRFQ"
BASE COUNT          280 a          168 c          208 g          238 t
ORIGIN      
        1 tcagtgctaa atattctaat cattatgaca ggcgagggag tgtccgatta tgaattcaaa
       61 aaaggtttgt tgcatatgtg tattattctc gctgcttgca ggatgtgcct ctcaatcttc
      121 tattgatgaa aaaaagaaaa aagctcaagt tacacaaagt aatattaata aaaacattcc
      181 cccgcaactg acagacaaag atttattcgg taatgaaacc actctggctg tatccgagga
      241 ggatattcag gctgcgcttg aaggagatga gtttcgtgtg cctctcaatt ctcccgtaat
      301 acttgtccag tctggcagtc gcgcaccgga aaccattatg caggaggaga tgagaaaata
      361 ttatactgtt tccacattct ctggtatccc ggacaggcaa aagcctctga cttgtaacaa
      421 aaataaagat aaaaatgaga acgaggatgt tgccagtgct gaaaatatga actggatgca
      481 ggcattgcgt tttgtggctg cgaaagggca tcagaaagcg attattgttt accatgatac
      541 gttgcagaca ggaaaatatg actctgcgct gaaatcaaca gtatggtccg actataaaaa
      601 tgaaaaactc actgacgcta tctccctgcg ctacctggta cgtttcacgc tggtggatgt
      661 ggcaacaggt gagtgggcta cctggtcgcc ggtgaattat gaatcgagag taatttttcc
      721 gccggctgga ataacaaaaa tgagcaataa agaactatca aatgaccatg ttacggaagt
      781 gcaactgttt aatttgaaac aaaaaacata tgtatcgatg gtgaaagact tggtgagtcg
      841 attccagtaa tcgttattgt tatgctgccg cctgtagttt agggcgggca gcaa
//
LOCUS       DAZFLD010000597          892 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.600, whole genome shotgun sequence.
ACCESSION   DAZFLD010000597 DAZFLD010000000
VERSION     DAZFLD010000597.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 892)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..892
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.600"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..319)
                     /gene="subB"
                     /locus_tag="ACHKBL_005286"
     CDS             complement(<1..319)
                     /gene="subB"
                     /locus_tag="ACHKBL_005286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_062863141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="subtilase AB5 cytotoxin subunit B"
                     /protein_id="HFO5821984.1"
                     /translation="MTIKRFFVCAGVMGCLSLNPAMAEWTGDARDGMFSGVVIDQFHT
                     GQIDNNPYFCIEGKQPGGSSIRACSMKNSSVWGPSFSTLYNQALYFYTTGQLVRIYYE
                     PGVW"
     gene            complement(336..>892)
                     /gene="subA"
                     /locus_tag="ACHKBL_005287"
     CDS             complement(336..>892)
                     /gene="subA"
                     /locus_tag="ACHKBL_005287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001380856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="subtilase AB5 cytotoxin subunit A"
                     /protein_id="HFO5821985.1"
                     /translation="ELLSRMGRNNERLIVAAVGNDGADIRKLSAQQRIWPAAYHPVSS
                     MNKKRDPVIRVAALAQYRKGETPVLHGGGITGSRFGNGWVDIAAPGQNITFLRPDGKT
                     GTGSGTSEATAIVSGVLAAMVSCNPRATATELKRTLLESADKYPSLADKVTEGRVLNA
                     EKAISMFCKKNYIPVRQGRMSEEL"
BASE COUNT          228 a          247 c          170 g          247 t
ORIGIN      
        1 tccatacccc gggttcataa taaatcctga ccaactgacc tgttgtgtaa aaatatagtg
       61 cctgattata taatgtggaa aacgaaggcc cccagacaga tgaattcttc atcgagcagg
      121 ctcttatgga agaaccacca ggttgtttcc cttcaataca aaaataaggg ttattatcta
      181 tttgccctgt atgaaactga tcaataacaa cgcctgaaaa catgccatcc cgggcatcgc
      241 cagtccactc agccatggca ggattaaggg ataaacatcc cataacacct gcacatacaa
      301 aaaacctctt aatcgtcata ttcacctccg gaattttaca gttcttcact catccttccc
      361 tgacggacag gaatataatt tttcttgcag aacatgctaa ttgctttttc tgcattaaga
      421 accctccctt ccgtcacctt atctgcaagt gaggggtatt tgtctgcaga ctccagcagt
      481 gtacgcttca gttctgtcgc tgttgcacgg ggattacatg agaccatcgc tgccagaacg
      541 ccggaaacta tagctgttgc ttccgaggtt ccactacctg tccccgtttt tccatctggt
      601 ctgaggaatg taatattctg tcccggtgca gcaatatcaa cccagccgtt tccgaaccga
      661 cttccggtga tccctccacc atgcaataca ggtgtttctc ctttccggta ctgtgccaga
      721 gctgccactc ttatcacagg atcccgcttt ttattcatac tgctgaccgg atggtaagcc
      781 gcaggccata ttctctgctg agcactcagt tttcgtatat ctgcgccatc attacccact
      841 gccgcaacaa ttaatcgctc attattccgg cccatacggg aaagcagttc gg
//
LOCUS       DAZFLD010000598          885 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.601, whole genome shotgun sequence.
ACCESSION   DAZFLD010000598 DAZFLD010000000
VERSION     DAZFLD010000598.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 885)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 885)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..885
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.601"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..524
                     /locus_tag="ACHKBL_005288"
     CDS             <1..524
                     /locus_tag="ACHKBL_005288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFO5821986.1"
                     /translation="QMNEGWYQIRIAGEDTAPEAVYARLHEQLGEGAVIHIVPRLAGA
                     GGNGVFQVVLGVAAIVGSFFTGGATMALWGAALSAGGLTATTMLFSLGVSMILGGVAQ
                     MLAPKAKTPEYKSTDNGKQNTYFSSLDNMIAQGNPMPVPYGEMLVGSRRISQDISTRD
                     EGGDGKVVVIGRG"
BASE COUNT          228 a          194 c          266 g          197 t
ORIGIN      
        1 gtcagatgaa cgaaggctgg taccagatac gtattgccgg tgaggatacc gcaccggagg
       61 cagtgtacgc ccgtcttcac gaacaactgg gtgagggagc ggtcatccac attgtaccgc
      121 gactggccgg agccggggga aatggtgttt ttcaggtggt gctgggggta gcagccatcg
      181 tgggctcttt cttcaccggc ggcgcaacga tggcgttgtg gggcgcagcc ctgagtgccg
      241 gagggctgac tgccaccacg atgctgttct cactgggtgt cagcatgata ctgggtggtg
      301 tggcccagat gctggccccg aaggcaaaga cgccggagta caaaagtacg gataacggta
      361 aacagaacac gtatttttcg tcactggaca acatgattgc ccagggtaac cctatgccgg
      421 tgccttatgg tgaaatgctg gttggttcac gacggatatc ccaggacatc agcacccgtg
      481 atgagggcgg agacgggaaa gtggtggtta tcgggcgggg ataaaaataa aaaaaatccc
      541 gcagagttag cggagctgcg ggaaataacg atgaagatta acgttatgga gttatttttt
      601 tgggcagaag aacagtaacg cagcgtcatt attgcggcta caggcaattg ccggaagtgt
      661 gaagaatttc agaaattttc tgttgcggag ctgaaataaa aaacgctccc gcaatacacc
      721 tggatattaa cgggaggaac gaatagtcac cttaaggagt tgcgattaat tgcttttatt
      781 gaccgcaggt aatctaccca tccggcaagg tttcctccac gggaatttgc cggaaatgtg
      841 aagaatttca gaaattttat tccgtcatga cacaggcacc ctccg
//
LOCUS       DAZFLD010000599          885 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.602, whole genome shotgun sequence.
ACCESSION   DAZFLD010000599 DAZFLD010000000
VERSION     DAZFLD010000599.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 885)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 885)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..885
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.602"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            241..624
                     /locus_tag="ACHKBL_005289"
     CDS             241..624
                     /locus_tag="ACHKBL_005289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5821987.1"
                     /translation="MGLDIYFLARDNHHSGAVVGTQEHQEVGAFRKVKPLLTWFREHT
                     GASDEQHAFPVTRSSLAALLRDLSMLTPGNCHRLFPYVTGSCLRGEDYAGDYWYDVDD
                     VKAWVSNTLKTFDFERRSLVMLALW"
     gene            693..>885
                     /locus_tag="ACHKBL_005290"
     CDS             693..>885
                     /locus_tag="ACHKBL_005290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000234971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArdC-like ssDNA-binding domain-containing
                     protein"
                     /protein_id="HFO5821988.1"
                     /translation="MFFRGHALMKTKKHTSRTATKTPERDLYQLVTDRVVTAIENGVP
                     PWKRPWRSARNGVGLPEMMP"
BASE COUNT          190 a          204 c          254 g          237 t
ORIGIN      
        1 ttgacagctt actgctgcag tttttgtccg gtcaggggcg acagttatcc ccggatgact
       61 gggcagcact ggaagaggcc gaatatcagc tttactggct gcgggctctt gccggtgggc
      121 ggtaacagaa cgccgtagtt tctttatccg gaataacgtc atcacgtatt ccgtcattat
      181 atcacccggc agggacatat ccctgtgggg agagtcccgg ctttattcag gagaattgtt
      241 atgggactcg atatttactt tctggctcgt gataaccatc acagtggtgc cgttgtggga
      301 acacaggaac atcaggaggt gggggctttc cgtaaggtga aaccgcttct gacatggttt
      361 cgtgaacata ccggtgcttc agatgagcag catgctttcc ctgtaacccg cagttcactg
      421 gctgcattat tgcgcgacct cagtatgctg acaccgggga actgccacag gctgtttcct
      481 tatgtcacag gctcgtgttt acggggggaa gattatgccg gtgattactg gtatgacgtg
      541 gatgacgtaa aagcgtgggt cagcaatacg ctgaagacgt ttgattttga gcgtcgttca
      601 ctggtgatgc tggcgttgtg gtaattctgg ccagtctgat tttcgttatt aatttaactg
      661 tacggtgcac tctcaccgta cagggagcgt gcatcttttt cagggggcat gctcttatga
      721 aaacgaaaaa acatacttca cgtaccgcga ctaaaactcc ggaacgtgac ctgtatcagc
      781 ttgtgaccga cagggttgtt acggccattg aaaatggtgt gccaccctgg aaacgtccct
      841 ggcgctctgc gcgtaatggc gtggggcttc cggagatgat gccgg
//
LOCUS       DAZFLD010000600          883 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.603, whole genome shotgun sequence.
ACCESSION   DAZFLD010000600 DAZFLD010000000
VERSION     DAZFLD010000600.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..883
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.603"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>883
                     /locus_tag="ACHKBL_005291"
     CDS             <1..>883
                     /locus_tag="ACHKBL_005291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000781922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HFO5821989.1"
                     /translation="VPAGSAEGDIVTLTVTKPDGKTETVSHKLTAEEVKDGKANVGIP
                     ADKVTQDGEYTVKAEITDPAGNTSGQGKATQFGVDTVAPSEPALKAENDGSVSATLPD
                     GANKGDKVEVTFTDEEGNEQKVTLEKGDGNWSSDKPELIPDSTDNKVTVPADKVKNNT
                     EVTATAKDPGGNESAPVTVTSKTDGVADAPALTIPEVADSVANAAELKDGLQAEVKLP
                     AGTVEGAVITLTVTHPDKTTRTETHTVSKDEAADGTVSMVVPKGSVVDGQNSVSVSLT
                     QGSNPAKAGNKVEFVVDG"
BASE COUNT          262 a          178 c          278 g          165 t
ORIGIN      
        1 ggttccggca ggcagtgctg aaggtgatat tgtgacgctg acggtcacca aaccggacgg
       61 gaaaacagaa acggtcagtc acaaacttac tgccgaagaa gtaaaagatg gtaaagcgaa
      121 tgtgggcatt cctgcggata aagtgacgca ggatggggaa tacacagtta aggctgaaat
      181 cacagacccg gcaggcaaca ccagtggtca gggtaaagct acccagtttg gcgtggatac
      241 tgtagctccg tcagaaccag cactgaaggc tgagaatgat ggttctgtat cagcgactct
      301 gccagatggt gcgaacaaag gtgacaaggt ggaagtgacc tttacggatg aagagggtaa
      361 tgagcagaaa gtcacgctgg agaaagggga tggcaactgg agttccgaca agcctgaact
      421 gatcccggac agcaccgata ataaagtgac agttccggca gacaaagtga agaacaacac
      481 agaggtgacg gcaacagcga aagatccggg tggtaatgaa agtgctccgg tcactgtgac
      541 gtcaaaaact gatggtgtgg cagatgctcc ggccctgacc attccagaag tggctgatag
      601 tgtggccaat gcggctgagc tgaaagatgg tttacaggca gaagtcaagt tgccggcagg
      661 aacagtggaa ggtgctgtca ttacactgac agtgactcat ccggataaaa ccaccagaac
      721 cgagacgcat actgttagca aagatgaggc tgcagacggt actgtgtcaa tggttgttcc
      781 gaagggaagt gttgttgacg gtcagaacag tgtgagtgtc agcctgactc agggcagtaa
      841 cccggctaaa gcggggaata aagtggagtt tgtggttgac ggg
//
LOCUS       DAZFLD010000601          882 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.604, whole genome shotgun sequence.
ACCESSION   DAZFLD010000601 DAZFLD010000000
VERSION     DAZFLD010000601.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..882
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.604"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..222
                     /locus_tag="ACHKBL_005292"
     CDS             <1..222
                     /locus_tag="ACHKBL_005292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head completion/stabilization protein"
                     /protein_id="HFO5821990.1"
                     /translation="GARMGDETQLVAQYKKAVYARAKADLMGEFATIGRRETYPGQES
                     EDTRATLLAEASLVMRNMLGHRRAGVHII"
     gene            219..692
                     /locus_tag="ACHKBL_005293"
     CDS             219..692
                     /locus_tag="ACHKBL_005293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO5821991.1"
                     /translation="MNQLESLTAFITENLPPDAMQFFMSSQEDAQLIRSQKALGNDQI
                     RMGVMTYTGVIAWDNFPFRKYSPGLVYALVMAWLEEHADPLREEFRPEDVNVDPQFND
                     EDSCLLDITVGLADPLIIRVQEDGPVPYRGKRWTLVNPEIWTAETMELFVSRSRE"
     gene            689..>882
                     /locus_tag="ACHKBL_005294"
     CDS             689..>882
                     /locus_tag="ACHKBL_005294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000623204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5821992.1"
                     /translation="MISGQLNPQQLAEAQAMLQSLDLPPKKRRRLLWRIARHGIIAAA
                     RRHQRDQTAPDGTAWAPRKRG"
BASE COUNT          202 a          256 c          274 g          150 t
ORIGIN      
        1 ggggccagga tgggcgatga aacccagctg gtcgcacagt acaaaaaggc cgtttatgcc
       61 cgtgccaagg ccgatcttat gggcgagttc gccaccattg gtcgccgtga aacctatccg
      121 gggcaggaaa gcgaggacac acgcgccacc ttactggctg aagcctcgct ggtcatgcgt
      181 aacatgctgg ggcacagacg cgcaggagtg cacatcatat gaaccagctg gaaagcctga
      241 ccgccttcat taccgaaaat ctgccgcctg acgccatgca gttcttcatg agcagtcagg
      301 aagacgcgca gcttatccgc agtcagaagg cgctgggtaa cgaccagatc cgcatgggcg
      361 tgatgacgta caccggcgtg attgcatggg ataacttccc gttccgtaaa tactcaccgg
      421 ggctggttta cgcgctggtc atggcctggc tggaagagca cgcggaccca ctgagggagg
      481 aattccgccc ggaggatgtg aacgtcgacc cgcagtttaa cgatgaggac tcgtgccttc
      541 tcgatatcac ggtgggactg gctgacccgc tgatcatccg ggtgcaggaa gacggccccg
      601 ttccgtacag ggggaaacgc tggacactgg ttaaccctga aatctggacg gcggaaacca
      661 tggagctgtt tgtcagcagg agccgcgaat gataagcggc cagctgaacc cgcagcagct
      721 ggcagaagcg caggcgatgc ttcagtcgct ggatctgcca ccaaaaaaac gccgtcgcct
      781 gttatggcgt attgcccggc acggcattat cgccgcagcc agacgccatc agcgcgatca
      841 gaccgcgccg gacggcacag catgggcacc ccggaaacgc gg
//
LOCUS       DAZFLD010000602          882 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.605, whole genome shotgun sequence.
ACCESSION   DAZFLD010000602 DAZFLD010000000
VERSION     DAZFLD010000602.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..882
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.605"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..222
                     /locus_tag="ACHKBL_005295"
     CDS             <1..222
                     /locus_tag="ACHKBL_005295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head completion/stabilization protein"
                     /protein_id="HFO5821993.1"
                     /translation="GARLGDETQLVAQYKKAVYARAKADLMGEFATIGRRETYPGQES
                     EDTRATLLAEASLVMRNMLGHRRAGVHII"
     gene            219..692
                     /locus_tag="ACHKBL_005296"
     CDS             219..692
                     /locus_tag="ACHKBL_005296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO5821994.1"
                     /translation="MNQLESLTAFITENLPPDAMQFFMSSQEDAQLIRSQKALGNDQI
                     RMGVMTYTGVIAWDNFPFRKYSPGLVYALVMAWLEEHADPLREEFRPEDVNVDPQFND
                     EDSCLLDITVGLADPLIIRVQEDGPVPYRGKRWTLVNPEIWTAETMDLFVSRSRE"
     gene            689..>882
                     /locus_tag="ACHKBL_005297"
     CDS             689..>882
                     /locus_tag="ACHKBL_005297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000623204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5821995.1"
                     /translation="MISGQLNPQQLAEAQAMLQSLDLPPKKRRRLLWRIARHGIIAAA
                     RRHQRDQAAPDGTAWAPRKRG"
BASE COUNT          197 a          258 c          274 g          153 t
ORIGIN      
        1 ggggccaggc tgggcgatga aacccagctg gtcgcacagt acaaaaaggc cgtttatgcc
       61 cgtgccaagg ccgatcttat gggcgagttc gccaccattg gtcgccgtga aacctatccg
      121 gggcaggaaa gcgaggacac acgcgccacc ttactggctg aagcctcgct ggtcatgcgt
      181 aacatgctgg ggcacagacg cgcgggagtg cacatcatat gaaccagctg gaaagcctga
      241 ccgccttcat taccgaaaat ctgccgcctg acgccatgca gttcttcatg agcagtcagg
      301 aagacgcgca gcttatccgc agtcagaagg cgctgggtaa cgaccagatc cgcatgggcg
      361 tgatgacgta caccggcgtg attgcatggg ataacttccc gttccgcaaa tactcaccgg
      421 ggctggttta cgcgctggtc atggcctggc tggaagagca cgcggacccg ctgagggagg
      481 aattccgccc ggaggatgtg aacgtcgacc cgcagtttaa cgatgaggac tcgtgccttc
      541 tcgatatcac ggtgggactg gctgacccgc tgatcatccg ggttcaggaa gacggccccg
      601 ttccgtacag ggggaaacgc tggacactgg ttaaccctga aatctggacg gcggaaacca
      661 tggatctgtt tgtcagcagg agccgcgaat gataagcggc cagctgaacc cgcagcagct
      721 ggcagaagca caggcgatgc ttcagtcgct ggatctgccc ccgaaaaaac gccgtcgcct
      781 gttatggcgt attgcccggc acggcattat cgccgctgcc agacgccatc agcgcgatca
      841 ggccgcaccg gacggtacag catgggcacc ccggaaacgc gg
//
LOCUS       DAZFLD010000603          880 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.606, whole genome shotgun sequence.
ACCESSION   DAZFLD010000603 DAZFLD010000000
VERSION     DAZFLD010000603.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 880)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 880)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..880
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.606"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(421..798)
                     /locus_tag="ACHKBL_005298"
     CDS             complement(421..798)
                     /locus_tag="ACHKBL_005298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000882415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFO5821996.1"
                     /translation="MLCLKYPEPEVVSAVHPAGSVFVLPPQGAPGISCTTRDNLERLR
                     GHLAAQLGRVECIRCQPQRVGLNSSVAVMLEGQQGQYVHILLTVSGHESWPSEEEYIH
                     PRWYISVTDAADLFYLLLWLGEG"
BASE COUNT          225 a          295 c          182 g          178 t
ORIGIN      
        1 ctggtttgat tattgtcgca gttattttat atcgctttcc catactggct ctcatctcaa
       61 caacaaaatc accggctgag ccatcactcc ggtaatgcat gcgaaaaaaa gtgagcgtct
      121 cagcaatggc tcatgcggtg attctgtacg gtacccgcac aaaatacggg caccaacagc
      181 gtcgcagaat acaacgaagg catcagggca gcacctctct cccgctattt ttcagagttc
      241 tttaccctcc tcctgacacg ggagtacaac cctgacagca tccgggccag tacattcgca
      301 agcacccatg aaaacacagc cacacccaca gctcccgcca gaaacagcac aggttcactg
      361 gcaatttgat ctgacagcga tggatacagt ggtcccggaa aatcgatatc gctcctcata
      421 tcacccctct cccagccaca acagcagata gaacaaatcc gccgcatcag tcactgatat
      481 ataccagcgg ggatgaatat actcctcctc cgacggccag ctctcatgcc cactcacagt
      541 aagcagaata tgcacatact gaccctgctg accttccagc atcaccgcca cactgctgtt
      601 cagacccacc ctctgtggct ggcaacggat acactccacc cgccccagtt gcgccgccag
      661 atgcccgcga agacgttcaa gattatcccg tgtggtgcaa cttatccccg gcgcaccctg
      721 tggcggcaga acaaacacac ttccggcggg gtgaacagca gacaccactt ccggttccgg
      781 atacttcaga cacagcatag tctcactccc tgccagccag accacgtagc ccgtccagct
      841 ttcgtgccac atgaaacgct gcttccagtt cgctgcactg
//
LOCUS       DAZFLD010000604          877 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.607, whole genome shotgun sequence.
ACCESSION   DAZFLD010000604 DAZFLD010000000
VERSION     DAZFLD010000604.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..877
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.607"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>877
                     /locus_tag="ACHKBL_005299"
     CDS             <1..>877
                     /locus_tag="ACHKBL_005299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011815933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFO5821997.1"
                     /translation="VPNNEAIVSVAQKSFGKEMAMIMFFAMVINIMIARFTPWKFIFL
                     TGHHTLFMSMMVAVILATAGMTGITLIAVGSLVVGVAMVFFPAIAHPYMKKVTGSDDV
                     AIGHFSTLSYVLAGFIGSKFGNKEHSTEDMNVPKSLLFLRDTPVAISFTMSIIFLVTC
                     LFAGADAVKELSGGKNWFMFSIMQSITFAAGVYIILQGVRMVIAEIVPAFKGISDKLV
                     PNARPALDCPVVFPYAPNAVLVGFLSSFAAGLIGMFTLYLLNMIVIIPGVVPHFFVGA
                     AAGVFGNATGGRRGA"
BASE COUNT          182 a          194 c          235 g          266 t
ORIGIN      
        1 tagtgccgaa caatgaagcc attgtttccg tagcgcagaa aagcttcggg aaagaaatgg
       61 cgatgattat gttctttgcg atggttatca acattatgat tgcccgtttc acaccgtgga
      121 aatttatctt cctgaccggt catcacacgt tgtttatgtc gatgatggta gcggtaattc
      181 tggcaacagc aggcatgacc ggcattacgc ttatagccgt tggctctttg gttgttggcg
      241 tggcaatggt ctttttcccg gctattgcgc atccgtacat gaagaaagtg accggttctg
      301 atgacgttgc catcggccac ttctcaacat tatcttatgt attggccggt ttcatcggca
      361 gcaagtttgg taataaagag cactcgactg aagacatgaa tgtgccgaaa agtctgctgt
      421 tcttgcgcga cacgccggtt gctatctctt ttaccatgag cataattttc ctggtgacgt
      481 gcctgtttgc gggtgcagat gcggtgaaag agcttagcgg tggtaaaaac tggttcatgt
      541 tctctatcat gcaatccatt accttcgcag ctggcgtgta catcattctg cagggtgtgc
      601 gcatggtgat tgcggagatt gtcccggcat tcaaaggtat ttcagacaag ctggttccga
      661 atgccagacc tgctctcgat tgcccggtcg tcttccctta cgcacctaac gctgtactgg
      721 ttggtttcct gagtagcttt gctgcaggtt taatcggtat gttcacgttg tatctgctga
      781 acatgatcgt gattattcct ggtgtggtgc cgcacttctt cgtaggtgca gctgcagggg
      841 tattcggtaa tgcaacgggt ggacgtcgtg gtgcgat
//
LOCUS       DAZFLD010000605          876 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.608, whole genome shotgun sequence.
ACCESSION   DAZFLD010000605 DAZFLD010000000
VERSION     DAZFLD010000605.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..876
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.608"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>876
                     /locus_tag="ACHKBL_005300"
     CDS             <1..>876
                     /locus_tag="ACHKBL_005300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000781922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RTX toxin"
                     /protein_id="HFO5821998.1"
                     /translation="VFNVVGENGQVTEAVLTQDGPVLENFTAGQSGTLSDDDRGGFIW
                     LGGLAFGGGLAAMALAAGGGGGSKHRHENHEADTTAPSAPELTAGDNGSVSVELPGDA
                     NKGDTVEITFEDENGDEQKVTLEKGDNGWTSSNPALIPDSQGDTATIAPDTVKDNSEV
                     TAVAKDPAGNESTPVTVTSKTDGVADAPVLTIPEVADSVANAAELKDGLQAEVKLPAG
                     TVEGAVITLTVTHPDQSTENVTHNVTGDEVTAGKVSMDIPEDAVVDGQNSVSVSLTQG
                     SNPAKAGNKVEFVVDG"
BASE COUNT          237 a          175 c          268 g          196 t
ORIGIN      
        1 gtatttaatg ttgtgggtga aaatggtcag gtaactgaag ctgtcctgac ccaggatggc
       61 cctgtactgg agaatttcac tgcaggacaa tccgggacct tgtcagatga tgatcgtggc
      121 ggttttatat ggttgggcgg tctggcattt ggtggggggc ttgctgctat ggctttggct
      181 gcaggtggtg gtggcggcag caagcacaga catgaaaacc atgaggcaga tacaacagca
      241 ccgtcagcac cggaattaac ggccggagat aatggttcag tttctgttga actgccgggt
      301 gatgcaaata aaggcgatac tgttgaaatc acctttgaag atgaaaatgg tgatgaacag
      361 aaagtcacac tggagaaagg ggataacggc tggacatcca gcaatcctgc actgatcccg
      421 gacagccagg gtgatacagc cacaatcgcc ccagacactg ttaaagataa cagtgaagtc
      481 acagcagttg ctaaagatcc ggctggtaat gaaagtactc cggtcactgt gacgtcaaaa
      541 actgatggtg tggcagatgc tccggtcctg accattccag aagtggctga tagtgtggcc
      601 aatgcggctg agctgaaaga tggtttacag gcagaagtca agttgccggc aggaacagtg
      661 gaaggtgcgg tcattacact gacagtgact catccggatc aaagtaccga aaatgtgaca
      721 cataacgtca ccggagatga ggttactgcc ggtaaggtat ccatggatat tcctgaggat
      781 gctgttgttg acggtcagaa cagtgtgagt gtcagcctga cccagggcag taatccggct
      841 aaagcgggga ataaagtgga gtttgtggtt gacggg
//
LOCUS       DAZFLD010000606          873 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.609, whole genome shotgun sequence.
ACCESSION   DAZFLD010000606 DAZFLD010000000
VERSION     DAZFLD010000606.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..873
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.609"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..165
                     /locus_tag="ACHKBL_005301"
     CDS             <1..165
                     /locus_tag="ACHKBL_005301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001506261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03752 family integrating conjugative element
                     protein"
                     /protein_id="HFO5821999.1"
                     /translation="QRYGQTFDAIYVPPGQTLAVHITRQLAIDYEDQGRRVRYDFTLP
                     GEVHGTGGLD"
     gene            201..593
                     /locus_tag="ACHKBL_005302"
     CDS             201..593
                     /locus_tag="ACHKBL_005302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004115530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03751 family conjugal transfer lipoprotein"
                     /protein_id="HFO5822000.1"
                     /translation="MTVMVAVFILSGCSTSKEELLPPGEQSMLEIWQGKGSGGHSRHM
                     TAARDTLRRPLTMPEQQADAHGAISYSRTQESEISQQFPRLPNPDMVMYVFPHLASGT
                     SPVPGYSTVFPFYSRTQYAMPGERTEAL"
     gene            677..>873
                     /locus_tag="ACHKBL_005303"
     CDS             677..>873
                     /locus_tag="ACHKBL_005303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraC family protein"
                     /protein_id="HFO5822001.1"
                     /translation="MKERVAPQVRKALSRPGKMTRQDETKVYHACPSVIDFLPWVEYL
                     DEEQCLLLDDGVSVGAVYEVSP"
BASE COUNT          195 a          209 c          280 g          189 t
ORIGIN      
        1 cagcgatacg ggcagacgtt tgatgccatt tatgttcccc cggggcagac gctggcggtg
       61 catatcacgc gtcaactggc cattgattat gaagatcagg ggcgccgggt acgttacgac
      121 ttcactctgc cgggggaagt ccacggtacc ggtggtctgg actgatgtaa ggagatcccg
      181 tgaaacgaca gttatctgta atgaccgtca tggtggcggt atttattctg tccggctgct
      241 cgacatcgaa agaggagctt ctgcctccgg gtgaacagag tatgctggag atctggcagg
      301 ggaaaggaag cggaggacat tcgcggcaca tgacagcggc cagggacacc ctgcggcgtc
      361 cgctgacaat gccggagcag caggcagatg cgcacggagc cataagctac agccggacac
      421 aggaaagtga gatcagtcag cagtttccgc ggctgcccaa tcctgacatg gtgatgtatg
      481 tttttccgca tctggcttcc ggtacgtcac cggttccggg gtacagcaca gtatttcctt
      541 tttacagccg gacgcagtat gccatgccgg gtgaacgaac ggaggcgttg tgatgcgatt
      601 ttccctgttt acagacacaa aaaaacgggt ttcttccccg gtgtcgcagc atgacggaga
      661 agcggtatct ccggatatga aagaaagagt ggcccctcag gtacgaaagg cactgtcaag
      721 gccggggaag atgacccgac aggatgagac gaaggtttac catgcctgtc cgtcggtgat
      781 tgattttctg ccatgggtgg aatatctgga cgaagagcag tgcctgctgc tggatgatgg
      841 ggtgtcggtt ggtgccgtgt atgaggtgag tcc
//
LOCUS       DAZFLD010000607          872 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.610, whole genome shotgun sequence.
ACCESSION   DAZFLD010000607 DAZFLD010000000
VERSION     DAZFLD010000607.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 872)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 872)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..872
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.610"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..296)
                     /locus_tag="ACHKBL_005304"
     CDS             complement(<1..296)
                     /locus_tag="ACHKBL_005304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HFO5822002.1"
                     /translation="MNPSDAIEAIEKPLSSLPYSLSRHILEHLRKLTSHEPVIGIMGK
                     SGAGKSSLCNALFQGEVTPVSDVHAGTREVRRFRLSGHGHSMVITDLPGVGESR"
BASE COUNT          219 a          180 c          221 g          252 t
ORIGIN      
        1 tcccggctct cgcccacccc gggcaggtca gtgataacca tgctgtgacc gtgaccactc
       61 agacggaagc gccgtacttc ccgggtgccg gcgtgaacat cactgaccgg ggtgacctcc
      121 ccctgaaaca gtgcattaca gagtgaggat ttaccggccc cgcttttacc cataatgcca
      181 atcacgggtt cgtgactggt gagtttacgc agatgttcca ggatgtgacg ggaaagtgag
      241 taaggcaggg aggaaagcgg tttttcaatt gcctcaatgg catcagacgg attcatacat
      301 gtattcccgg tgaaaaaaga caaaaccccg cgcaccggaa aggtggcggg ggatccacgg
      361 agataatatc tgtctgaaat tgttttgaat cgatgggatt gttgcgtatt aacatgaagg
      421 agatcaggac ttatccgtta ctgggtgatc tccttccatg aaaatacgcg gttattgtta
      481 aacagcaggc tgattactcc gtaaaggaga aaaatattca tcttgcagcc tgattatcag
      541 gtgtgtttat gtttactgag gtgagtattt ttcagtatat tgtcggggca gtcagtggga
      601 gatattaaat ttacgtttag agttatcttg cttctgctgt gtatttttat aagagtgcgc
      661 acgtaatgat atctgttgat accatgttgt taagtgtttc ccttccgggt catgcgtact
      721 tgcaaaatgg catctgaatc gatcacggtt tggccaaacc gtgatcgaat agagaggaat
      781 acgcacgtaa tgattaaatg cttttgttac accattcttc cggcatgaat atttatccag
      841 ttcccttttt tatttgtgta gtgatactgt cg
//
LOCUS       DAZFLD010000608          868 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.611, whole genome shotgun sequence.
ACCESSION   DAZFLD010000608 DAZFLD010000000
VERSION     DAZFLD010000608.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 868)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 868)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..868
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.611"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            16..372
                     /locus_tag="ACHKBL_005305"
     CDS             16..372
                     /locus_tag="ACHKBL_005305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFO5822003.1"
                     /translation="MQWYLAVLKNYVGFSGRARRKEYWMFTLINAIVGAIINVIQLIL
                     GLEFPFLSLIYLAATIIPVIALCVRRLHDTDRSGAWALLYLVPIIGWLVLFVFACLEG
                     NSGSNRYGNDPKFGSN"
     gene            complement(423..>868)
                     /gene="yhaJ"
                     /locus_tag="ACHKBL_005306"
     CDS             complement(423..>868)
                     /gene="yhaJ"
                     /locus_tag="ACHKBL_005306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YhaJ"
                     /protein_id="HFO5822004.1"
                     /translation="PDMHFRSSSEINSRKLYTLMNVYVAAPDHPIHQEPEPLSEVTRV
                     KYRGIAVADTARERPVLTVQLLDKQPRLTVSTIEDKRQALLAGLGVATMPYPMVEKDI
                     AEGRLRVVSPESTSEIDIIMAWRRDSMGEAKSWCLREIPKLFSGK"
BASE COUNT          194 a          219 c          200 g          255 t
ORIGIN      
        1 taaagggatg tttttatgca gtggtattta gccgtattaa aaaattatgt tggtttctct
       61 ggtcgcgcac gtcgtaaaga gtactggatg tttactctga ttaacgccat cgtcggcgct
      121 attatcaatg tcattcaatt gattttaggt ttagagtttc cattcctttc tctcatttac
      181 ctggcagcaa caatcattcc ggtcattgct ctgtgcgttc gtcgtctgca cgatactgac
      241 cgttcaggtg cgtgggcgct tctctatctg gttccgatca ttggctggtt agttctgttt
      301 gtatttgcct gtttagaagg caactctggc agcaatcgtt acggaaacga tccgaagttt
      361 ggttcaaatt aattatagaa ttggggcgat atttcgcccc tttttattaa caataacaaa
      421 tattattttc cgctaaaaag tttgggaatt tcccgcagac accaggattt cgcctccccc
      481 atactgtcac gacgccaggc cataataata tcgatctcgc tggtcgattc cgggctgacg
      541 acacgcaacc gcccttccgc gatgtctttt tcgaccatcg gatacggcat cgtcgccacg
      601 ccaagccccg ccagtaacgc ctgacgttta tcttcaatcg tgctcaccgt taaacgcggc
      661 tgtttgtcca gtagctgtac ggtcaatacc gggcgctcac gagcggtatc cgccaccgca
      721 attccacgat atttcacgcg cgtcacttca gataacggtt ccggctcctg atgaatcggg
      781 tgatcaggcg cggcaacgta gacgttcatt aacgtataga gcttgcgcga gttgatctcc
      841 gacgaggagc gaaaatgcat atccggcg
//
LOCUS       DAZFLD010000609          854 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.612, whole genome shotgun sequence.
ACCESSION   DAZFLD010000609 DAZFLD010000000
VERSION     DAZFLD010000609.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 854)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 854)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..854
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.612"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>854
                     /locus_tag="ACHKBL_005307"
     CDS             <1..>854
                     /locus_tag="ACHKBL_005307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HFO5822005.1"
                     /translation="GLITRITTPDGRASAFYYNHHSQLTSATGPDGLELRREYDESGR
                     LIQETAPDGDITRYRYDNLHSDLPCATEDATGSRKTMTWSRYGQLLTVTDCSGYVTRY
                     DHDRFGQMTAAHREEGLSQYRAYDSRGQLIAVKDMQGHEMRYEYNAAGDLTAVIAPDG
                     SRNGTQYDTWGKAIRTTQGGLTRSMEYDAAGRVIRLTSENGSHTTFRYDVLDRLIQET
                     GFDGRTQRYHHDLTGKLIRSEDEGLVTHWHYDEADRITHRTVKGETAEQWRYDERGWL
                     TQISHLSEG"
BASE COUNT          216 a          249 c          244 g          145 t
ORIGIN      
        1 ggactcatca cgcgcatcac cacaccggat ggcagggcat cggcatttta ctataaccac
       61 cacagccagt taacgtcagc caccgggcct gacgggctgg aattgcgccg ggaatatgat
      121 gaatccggcc gtctgattca ggaaactgcc cctgacggcg atatcacccg ctaccgttat
      181 gataatctac acagtgactt accctgcgca acggaagatg ccaccggcag ccggaaaacc
      241 atgacatgga gccgttacgg tcagttgctg accgtcactg actgttccgg ttatgtaacc
      301 cgttatgacc atgaccgttt tggtcagatg acggcggcgc accgcgagga agggctgagt
      361 cagtatcgcg catacgacag ccgtggacag ttaattgcgg tgaaagacat gcagggccat
      421 gaaatgcggt atgaatacaa cgccgccggt gacctgaccg ccgtcattgc cccggacggc
      481 agcagaaacg ggacacagta cgatacgtgg ggaaaggcca tccgtaccac gcagggtggt
      541 ctgacgcgca gtatggaata cgatgctgcc ggacgggtca tccgcctgac cagtgaaaac
      601 ggcagccaca ccaccttccg ttacgatgta ctcgaccggc tgatacagga aaccggcttt
      661 gacggccgca cacagcgtta tcatcacgac ctgaccggta aacttatccg cagcgaggat
      721 gaggggctgg tcacccactg gcactatgac gaagcagacc gcatcacgca ccgcaccgtg
      781 aagggtgaaa ccgcagaaca gtggcggtac gacgaacgcg gctggctgac acaaatcagc
      841 catctcagcg aagg
//
LOCUS       DAZFLD010000610          854 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.613, whole genome shotgun sequence.
ACCESSION   DAZFLD010000610 DAZFLD010000000
VERSION     DAZFLD010000610.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 854)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 854)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..854
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.613"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>854
                     /locus_tag="ACHKBL_005308"
     CDS             <1..>854
                     /locus_tag="ACHKBL_005308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HFO5822006.1"
                     /translation="GLITRITTPDGRASAFYYNHHSQLTSATGTDGLELRREYDESGR
                     LIQETAPDGDITRYRYDNPHSDLPCATDDATGSRKTMTWSRYGQLLTVTDCSGYVTRY
                     DHDRFGQMTAAHREEGLSQYRAYDSRGQLIAVKDTQGHETRYEYNAAGDLTAVIAPDG
                     SRNGTQYDTWGKAIRTTQGGLTRSMEYDAAGRVIRLTSENGSHTTFRYDVLDRLIQET
                     GFDGRTQRYHHDLTGKLIRSEDEGLVTHWHYDEADRITHRTVKGETAEQWRYDERGWL
                     TGISHLSEG"
BASE COUNT          212 a          250 c          249 g          143 t
ORIGIN      
        1 ggactcatca cgcgcatcac cacgccggat ggcagggcat cggcgtttta ctataaccac
       61 cacagccagt taacgtcagc caccgggact gacgggctgg agttgcgcag ggaatatgat
      121 gaatccggcc gtctgattca ggaaactgcc cctgacggcg atatcacccg ctaccgttat
      181 gataatccac acagtgactt accctgcgca acggacgatg ccaccggcag ccggaaaacc
      241 atgacatgga gccgttacgg tcagttgctg accgtcactg actgttccgg ttatgtaacc
      301 cgttatgacc atgaccgttt tggtcagatg acggcggcgc accgcgagga agggctgagt
      361 cagtatcgcg catacgacag ccgtggacag ttaattgcgg tgaaagacac gcagggccat
      421 gaaacgcggt atgaatacaa cgccgccggt gacctgaccg ccgtcattgc cccggacggc
      481 agcagaaacg ggacacagta cgatacgtgg ggaaaggcca tccgtaccac gcagggtggt
      541 ctgacgcgca gtatggaata cgatgctgcc ggacgggtca tccgcctgac cagtgaaaac
      601 ggcagccaca ccaccttccg ttacgatgta ctcgaccggc tgatacagga aaccggtttt
      661 gacggccgca cacagcgtta tcatcacgac ctgaccggta aacttatccg cagcgaggat
      721 gaggggctgg tcacccactg gcactatgac gaagcagacc gcatcacgca ccgcaccgtg
      781 aagggtgaaa ccgcagaaca gtggcggtac gacgaacgcg gctggctgac aggcatcagc
      841 catctcagcg aagg
//
LOCUS       DAZFLD010000611          848 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.614, whole genome shotgun sequence.
ACCESSION   DAZFLD010000611 DAZFLD010000000
VERSION     DAZFLD010000611.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 848)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 848)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..848
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.614"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..332)
                     /locus_tag="ACHKBL_005309"
     CDS             complement(<1..332)
                     /locus_tag="ACHKBL_005309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000009550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase"
                     /protein_id="HFO5822007.1"
                     /translation="MSFNPTDEQYAIINWQGQQLVVNAFSGTGKTSTLVQFARASPDS
                     RMLYLAYNRAIRDEAERKFPFNVECRTSHQLAYARVGRHYRHRLVPGLRITDVARKLN
                     TRYWALARV"
     gene            564..>848
                     /locus_tag="ACHKBL_005310"
     CDS             564..>848
                     /locus_tag="ACHKBL_005310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5822008.1"
                     /translation="MSEVKKHVSYSLTVAAMLSAAIVCQTAHADVNTPNITGEKNNTT
                     TRFSGNIYGSENTVTSESNSLVVGNSNSVEGGYNNIVIGNSSTVKASGLIR"
BASE COUNT          240 a          217 c          183 g          208 t
ORIGIN      
        1 acccgtgcca gagcccagta tcgtgtattc agctttctcg ccacatcagt aatacgtaat
       61 cccggaacca gacgatgacg gtagtgtctt ccaacccggg catacgccag ctgatgtgac
      121 gttctgcatt ccacgttaaa gggaaattta cgctctgcct catcacgtat tgcccggtta
      181 taggcaagat agagcatacg gctgtccgga ctggcccggg caaactgtac cagcgtactc
      241 gtcttccctg tgccggaaaa cgcattcaca accagttgct gcccctgcca gttaataatg
      301 gcgtactgtt catctgtggg attaaaagac atgtattctc ttctccggaa aaacatttcc
      361 gtaatctgat gcatcagggg tatgcccacc acaaataact ctgtacagca acagaaaaat
      421 ttccctggtc atatgatgaa ttaatcatcg ccatcacacc ggatgcttct catcctgtcc
      481 tcagaaaggc aggtggtctc ctgtctgccg cgcttatcca ggataccggg acctgaaagg
      541 tcataatctg gcaggaaaaa ataatgtccg aagtaaaaaa acacgtttct tattcattaa
      601 cagttgcagc tatgctgtca gccgccatcg tctgtcagac agcacatgct gatgtcaaca
      661 caccgaacat caccggagaa aaaaacaata caacaacacg attctccgga aatatttacg
      721 gcagtgaaaa tactgtcaca tcagagtcaa acagtctggt tgtgggtaac agtaattccg
      781 tggagggagg gtataacaac attgttattg gtaattcctc cacggtcaaa gcttccggtt
      841 tgatcaga
//
LOCUS       DAZFLD010000612          832 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.615, whole genome shotgun sequence.
ACCESSION   DAZFLD010000612 DAZFLD010000000
VERSION     DAZFLD010000612.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..832
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.615"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>832
                     /locus_tag="ACHKBL_005311"
     CDS             <1..>832
                     /locus_tag="ACHKBL_005311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001367806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemagglutinin"
                     /protein_id="HFO5822009.1"
                     /translation="AGFALKDSKGQEVKQSLGQSIKVSGDSNITTTAITNGDKGLKIS
                     LNNNLSVGSNNTPGSIKVNGADGKEAVSLNGQDGTIKLTSAPAANGKSASATIAVNAG
                     KGGLTDAAGTTKTRLTYAPDGTLPAEELATMNDGLSFEGDDGKVIHKKLNEKLTIKGG
                     AGDNEDVTDSNIRVDANEKGDGLLVRMTRNLRDLASAVFGTGGQITTVNDKGITITTP
                     AAGGGQGNTVSLTGSGLNNGGNQITSVASGLNGTALADAGGDVLNNAANIGDLKNALR
                     D"
BASE COUNT          243 a          174 c          234 g          181 t
ORIGIN      
        1 gcaggtttcg cactgaaaga ttctaaggga caggaagtga aacagtctct tgggcagtcg
       61 attaaagtat ccggggacag caatatcacc accacagcca tcaccaatgg ggataagggg
      121 ttaaaaatat ccctgaataa taacctgagc gttgggagca ataatactcc cggcagcatc
      181 aaagttaacg gggctgatgg taaggaggct gtgagtctga atggtcagga tggcacgatt
      241 aaactgacat ccgcgcctgc tgcgaacgga aagtctgcgt cagcaaccat tgctgttaat
      301 gcaggcaaag gtggattaac agatgctgcc ggtaccacga agacacgtct gacttatgct
      361 ccagatggaa cattaccggc agaagagctg gcgaccatga atgacggtct ttcttttgag
      421 ggtgatgacg gaaaggttat tcataaaaaa ctgaatgaaa aactgaccat caaaggtggt
      481 gctggtgata atgaggatgt aacagacagt aacattcgtg ttgatgcaaa tgaaaaggga
      541 gatggtcttc tcgtccggat gaccagaaac ctccgtgatt tggccagcgc ggtttttggt
      601 acaggtgggc agataacaac agttaatgat aaaggcatca caatcacaac accggctgcg
      661 ggcggtggtc aggggaatac agtttccctt accggctccg gcctgaataa cggtggaaat
      721 cagataacca gtgtggccag tggtctgaac ggcactgcgc ttgctgacgc cggtggagat
      781 gttctgaata atgcggccaa tatcggtgac ctgaaaaatg ctctccgtga ca
//
LOCUS       DAZFLD010000613          829 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.616, whole genome shotgun sequence.
ACCESSION   DAZFLD010000613 DAZFLD010000000
VERSION     DAZFLD010000613.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..829
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.616"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            682..>829
                     /locus_tag="ACHKBL_005312"
     CDS             682..>829
                     /locus_tag="ACHKBL_005312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001544930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO5822010.1"
                     /translation="MKRSGFEVQKVTVEALFLREIRTRFGKFRLGYLWAILEPAAHLL
                     VLLGI"
BASE COUNT          256 a          142 c          187 g          244 t
ORIGIN      
        1 taagagtaag ctattagatg taaattactt tggtcaggtc cttcacagca gaaacagaat
       61 gtcatgaaag ataaatttac gcagtcactt gatcaactgc ttagttttgc gtcttaaagg
      121 attgtaacta tggcaactgt ggagcgaaaa atcattgggc acgggaaaag tgcggtcata
      181 tagaaaagtg ctctctattc agcatctcat ggtttatcag cgaagacaga aaaaaattat
      241 aacgatgcgc aggaatttgt ggaggtttgc taatatagag cgattcgagc atccctcgtt
      301 aaatctctcg aaccgcagta tattcagaca tttttaaaat acgccagatg attacgacag
      361 aaagaacaca acatataatt catgttcgct gtcctcttgc tgaatacgga attgtcttca
      421 ataaaggtgc tacacattat ggtgagagct accacggttt cttgaggatg cagaaaatgg
      481 atttaccgat atcgtgcaat tttgtatcgg tagtatgtcc gactcatgtc tcatggataa
      541 tgaaccatat attataactg cgggtagaaa aatgcgcatc taacgacttt gcctggacgt
      601 gtcgattaac tgttcctggg tttgctactc ttgtcagtca gacgataaaa gtgagcataa
      661 atatatagga ttacatatca agtgaaaaga agtggatttg aagttcagaa ggtgacagta
      721 gaggcattat ttctacgaga aatacgaaca cgttttggta agtttcgtct gggatatctg
      781 tgggcaatcc tggaacccgc tgctcatttg ctggtgctgt taggtattt
//
LOCUS       DAZFLD010000614          828 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.617, whole genome shotgun sequence.
ACCESSION   DAZFLD010000614 DAZFLD010000000
VERSION     DAZFLD010000614.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 828)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 828)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..828
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.617"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            153..629
                     /locus_tag="ACHKBL_005313"
     CDS             153..629
                     /locus_tag="ACHKBL_005313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001165712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR family transcriptional regulator"
                     /protein_id="HFO5822011.1"
                     /translation="MQIIIMTRDRYLEYGLMRMLNGYRLTTGRELFDAGKRRLPLPED
                     SYVILCDRNLERLTYCMFCGRRFLVIPVSSVRCLTDIRQAIRRGAWLFGHTARPLTRT
                     EMVVVFGVIFHEYGCTFLADQLGISMKTVCAHLYNAMEKSGLRGVSVKYLCSTADR"
BASE COUNT          197 a          159 c          248 g          224 t
ORIGIN      
        1 gaaggagaag gggaactgaa agagctgagt ctgtcagagc tggaagagcg ggcactgatg
       61 tatgcggcag acagtttccg ttatgaatga tgagtcagtt ataccggtaa tggtgaacgg
      121 agccggtatc cgggagataa gggtctgaga gtatgcagat tattattatg acccgtgaca
      181 gatatctgga atatggcctg atgcgtatgc tgaacggata caggctcacg acaggccgtg
      241 aactgtttga tgccggaaag cgccgtctgc cacttcccga agacagttat gtgattctct
      301 gtgaccgtaa tctggaaagg cttacatact gtatgttctg tgggcgtcgt tttcttgtca
      361 ttcctgtttc ctctgtgaga tgcctgacag atatcaggca ggccatccgt cgtggtgcgt
      421 ggctgttcgg acatacggca agaccactga cccgtaccga gatggtggtg gtcttcgggg
      481 ttattttcca tgagtacggc tgtacctttc tggcagacca gctggggata agcatgaaga
      541 cggtatgtgc gcatctttac aatgcgatgg agaaaagcgg tctgcgaggt gtcagtgtta
      601 aatatctctg cagcaccgca gaccggtaaa aagccggtct gatgaaggct gttgcgacgg
      661 gaatttctgt gcatgctgtg tcacgggcat cccggtgctc cggataatta atgttatgtg
      721 gtcaggcgtg ataaatttca tgtggaacgg gtatgcgttt tatttgtgat aacagttatt
      781 ttctggaggg agtgaaggag tatattaatc ccggtaaatt cattatca
//
LOCUS       DAZFLD010000615          824 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.618, whole genome shotgun sequence.
ACCESSION   DAZFLD010000615 DAZFLD010000000
VERSION     DAZFLD010000615.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 824)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 824)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..824
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.618"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(141..704)
                     /gene="ipbA"
                     /locus_tag="ACHKBL_005314"
                     /gene_synonym="fimX"
     CDS             complement(141..704)
                     /gene="ipbA"
                     /locus_tag="ACHKBL_005314"
                     /gene_synonym="fimX"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_060710320.1"
                     /note="IpbA is also known as HbiF and FimX; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type DNA invertase IpbA"
                     /protein_id="HFO5822012.1"
                     /translation="MTRKYLTQDEVYRLMDAAQSMSFPERNRCLIMMAFIHGFRASEL
                     LDLRLSDIDASGKQLNIRRIKNGFSTTHPLLPDEYNLIKLWLKQRKLIENGVEGDWLF
                     LSRKCRPISRQHFFSIIREAGRRAGLAVKAHPHMLRHACGFALADNGVDTRLLQDYLG
                     HRNIQHTVRYTASNAARFKGVWKKKPR"
BASE COUNT          247 a          161 c          163 g          253 t
ORIGIN      
        1 catttcaaat catggggtta ttttacttta gcgagaaata tcaacatgtt atgtgtttta
       61 tttaattgaa tgagctgttt ttttatggaa gaattgaggc aaacgtagga atctgacatt
      121 ttgaatcaga aggtactgtt ttaacgaggc ttttttttcc acaccccttt aaatcgagca
      181 gcattactgg ctgtgtatct gacagtatgc tgaatatttc gatgacccag ataatcctga
      241 agtagtcggg tatcgacacc attgtcagcc agagcaaatc cacaggcatg acgtaacata
      301 tgaggatgtg cttttacagc taatcctgca cgtcttcctg cctcacgaat gatcgaaaaa
      361 aaatgttgtc tgctgatagg gcggcatttc cgggagagaa aaagccagtc tccttcaact
      421 ccattttcaa ttaacttcct ctgcttaagc cacaacttga tcaaattata ttcatcagga
      481 agaagaggat gtgttgtcga gaagccattt ttaatacgcc ggatattaag ttgttttcct
      541 gatgcatcaa tatcggataa acgtaaatca agaagttcac tggccctaaa accgtgaatg
      601 aaagccatca taattagaca gcgatttctt tcaggaaagg acatgctctg ggctgcatcc
      661 atcagcctgt agacttcatc ctgtgtgaga tattttctcg tcatacgcca ccgtgtacag
      721 ttgtaaaaaa aagaggcaca cccagataag tgtgcctgca atcagaacgt tatcgatgta
      781 atttcattat ctcattaata agattttttg aatagtagtt ctac
//
LOCUS       DAZFLD010000616          824 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.619, whole genome shotgun sequence.
ACCESSION   DAZFLD010000616 DAZFLD010000000
VERSION     DAZFLD010000616.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 824)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 824)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..824
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.619"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            117..>824
                     /gene="reiD"
                     /locus_tag="ACHKBL_005315"
     CDS             117..>824
                     /gene="reiD"
                     /locus_tag="ACHKBL_005315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000332219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="myo-inositol utilization transcriptional
                     regulator ReiD"
                     /protein_id="HFO5822013.1"
                     /translation="MAYVSQVSESNGPFVRFMHMHPRTVELILITEGKGEYFIGDRIY
                     PVRKGDLVIYNSEVVHDEYLESGRPVGTICCGINNIVCPGLRENALIPDDTVPVIPLY
                     HHYATIEKLMSSIFNVINQHTVEGPTMAQLLTRVLLKYVEGNVLMRTGNDITRRHKNL
                     LDSIKAYIDHNFYEPIRLDALATKFNVSPYYVSHEFKRRYGYSPMDYLIKRRLGEAQS
                     LLTTDEGGCEKITSIAYR"
BASE COUNT          239 a          151 c          195 g          239 t
ORIGIN      
        1 tattataaaa aaatagtttt agttttggcc gtagcactac agattcggat aatggaggaa
       61 aaatcatgcg tttttgcttc tgtccttcag aacaagataa taagtcccgg gctcagttgg
      121 cgtatgtcag tcaggttagc gaaagcaatg gcccgtttgt acggttcatg catatgcatc
      181 ccaggaccgt ggagctaatc cttatcaccg agggaaaggg ggagtatttc atcggtgatc
      241 gcatttaccc agtcagaaaa ggtgatttgg ttatttataa cagcgaagta gtgcatgatg
      301 agtatcttga aagcggacgg cctgtgggta ctatttgctg tgggataaac aatattgtgt
      361 gcccgggtct gcgggagaat gctctgattc cagatgatac tgtgcccgta attccactat
      421 atcatcatta tgcgacgatc gagaaattga tgagttcaat atttaatgtt ataaatcagc
      481 atacagtaga aggaccaaca atggcgcagt tgctgaccag ggtgctgctt aaatacgtcg
      541 agggaaatgt attaatgcgc acaggtaatg atataacacg gcgccataaa aatttactgg
      601 attcaataaa agcttatatt gaccataatt tctatgaacc catccgtctg gatgctctgg
      661 cgactaagtt taatgtcagc ccttactatg tttctcatga atttaaacgg cgctatggat
      721 attcgccaat ggactatttg attaaacgtc ggttagggga ggcacaatct ttacttacca
      781 ctgatgaagg tgggtgtgaa aaaattacct caattgccta tcgt
//
LOCUS       DAZFLD010000617          821 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.620, whole genome shotgun sequence.
ACCESSION   DAZFLD010000617 DAZFLD010000000
VERSION     DAZFLD010000617.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..821
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.620"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            44..577
                     /locus_tag="ACHKBL_005316"
     CDS             44..577
                     /locus_tag="ACHKBL_005316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFO5822014.1"
                     /translation="MSRKFRYGLSAVVLAMIAAGASAPEILDQFLDEKEGNRTTAYRD
                     GAGIWTICRGATRVDGKPVVPGMKLSKEKCAQVNAIERDKALAWVEKNIKVPLTEPQK
                     AGIASFCPYNIGPGKCLPSTFYKRLNAGDRKGACESIRWWIKDGGRDCRTRSNNCYGQ
                     VIRRDQESALACWGIDQ"
     gene            574..>821
                     /locus_tag="ACHKBL_005317"
     CDS             574..>821
                     /locus_tag="ACHKBL_005317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysis system i-spanin subunit Rz"
                     /protein_id="HFO5822015.1"
                     /translation="MRRVTAIIYALVICIIVCLLWAVNHYRDNAITYKAQRDKKAREL
                     ELANATITDMQQRQRDVAALDARYSRELADARAENETLR"
BASE COUNT          208 a          187 c          241 g          185 t
ORIGIN      
        1 attaaagaag accggcgtaa ggcggcgcgg ggagagtaag ctgatgagca ggaaattccg
       61 ctatggttta tcagcggtcg ttctggcgat gattgctgca ggtgcttctg cgcctgaaat
      121 cctcgaccag tttcttgacg aaaaagaagg taatcgcacc acagcatacc gtgatggtgc
      181 gggtatctgg accatctgcc gtggtgccac ccgggtggat ggtaagcccg tcgttcccgg
      241 catgaagctg tcgaaggaaa aatgcgcaca ggttaatgcc attgagcgtg ataaggcgct
      301 ggcatgggtg gagaaaaaca tcaaagtgcc actgaccgaa ccccagaaag cgggtattgc
      361 gtcattctgc ccgtataaca ttggccccgg taagtgtctc ccgtcgacgt tttataagcg
      421 gcttaatgcc ggtgatcgta aaggtgcctg tgagtcgatt cgctggtgga ttaaggacgg
      481 tgggcgtgat tgccgcacac gttcaaataa ctgctacgga caggttattc gtcgtgacca
      541 ggaaagcgca ttagcttgtt gggggataga tcagtgagaa gagtaaccgc gattatttac
      601 gctctggtta tctgcatcat cgtctgtctg ttatgggctg ttaatcatta ccgcgataac
      661 gccatcacct acaaagcgca gcgcgataaa aaagccagag agctggagct ggcaaacgca
      721 accattactg acatgcagca gcgccagcgt gatgttgctg cgcttgatgc cagatactca
      781 agggaattag ccgatgcgag agctgaaaat gaaactctgc g
//
LOCUS       DAZFLD010000618          819 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.621, whole genome shotgun sequence.
ACCESSION   DAZFLD010000618 DAZFLD010000000
VERSION     DAZFLD010000618.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..819
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.621"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>819
                     /locus_tag="ACHKBL_005318"
     CDS             <1..>819
                     /locus_tag="ACHKBL_005318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000005623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5822016.1"
                     /translation="GNATRINGDSSVAIGLTSSVTGNDSVALGSHSSANGNNIVSVGS
                     DTLKRRITNLAQGKDDHDAVNLMQMTSAVNREAGERIKSTNNLQNQLNTLSGQVITET
                     RARTEGDAAALAAARAHSDENDKRTLVSANTYADQKETAINNRTDGLLTTERQARTAG
                     DTETLAAARTHSDENDKRTLVSANTYADQKETAINNRTDGLLMTERQARIAGDTATLA
                     AARTHSDENNKRTLVSANTYADQKETDINNRTDGLLMTERQARIAGDAETLATA"
BASE COUNT          266 a          218 c          201 g          134 t
ORIGIN      
        1 tcggtaatgc caccagaatt aacggtgatt catctgttgc cataggtctg acatcgtccg
       61 taaccgggaa cgactccgtg gcactgggca gtcattccag tgctaacgga aacaatatcg
      121 tatccgtcgg ctctgacaca ctgaaacgcc gcatcacaaa tctggcacag gggaaagatg
      181 accatgatgc ggtaaatctg atgcagatga ccagtgctgt taaccgggaa gctggagaaa
      241 gaataaaatc aacaaacaac ctgcagaatc agcttaatac cctctctggt caggtcataa
      301 ctgaaaccag ggcccgaaca gaaggtgatg cagcagcgct ggctgcagcc agagcccaca
      361 gcgatgaaaa cgacaaaaga acactggtca gtgcgaatac ctatgctgac cagaaagaaa
      421 ccgccatcaa taaccgtact gacggattac tgacgaccga acgccaggca cgcaccgcgg
      481 gcgatactga aactctggct gctgccagaa cgcacagcga tgaaaacgac aaaagaacac
      541 tggtcagtgc gaatacttat gctgaccaga aagaaaccgc catcaataac cgtactgacg
      601 gattactgat gaccgaacgc caggcacgca tcgcgggcga tactgcaact ctggctgctg
      661 ccagaacgca cagcgatgaa aacaacaaaa gaacactggt cagtgcgaat acctatgctg
      721 accagaaaga aaccgacatc aataaccgta ctgacggatt actgatgacc gaacgccagg
      781 cacgcatcgc aggtgatgca gaaactctgg caacagcag
//
LOCUS       DAZFLD010000619          818 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.622, whole genome shotgun sequence.
ACCESSION   DAZFLD010000619 DAZFLD010000000
VERSION     DAZFLD010000619.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..818
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.622"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>818
                     /locus_tag="ACHKBL_005319"
     CDS             <1..>818
                     /locus_tag="ACHKBL_005319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000231765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraC family protein"
                     /protein_id="HFO5822017.1"
                     /translation="RDTVEDALQDSFDEYDSHPWVVQFFCQDENDVDTYVDQLRGYVK
                     PHAEGSSFTEAWLREMERHLKGIARPEGLFRDTLVTGQPWRGQQRRTRMVIYRWIGKN
                     NHDPMPPVAMLNQVCSRVVGALGGAGVRCTRMNGQQVHGWLLRLFNPRPEWVDRDILY
                     RMASRAEPQETPEGMMPVMTDFAESLWFTPPVSDPENGVWWLDGLPHAAVVVEKLRTP
                     PEPGTITGEQARGEKTVNALMDTFPEGTVLCMTIVVQPQDTLEERFTRLSKNAVG"
BASE COUNT          180 a          198 c          286 g          154 t
ORIGIN      
        1 cgggatacgg tggaggatgc gctgcaggac agttttgatg aatatgactc acatccctgg
       61 gtggtgcagt tcttctgtca ggatgaaaac gacgtggaca cgtatgttga ccagctcagg
      121 gggtacgtga aacctcatgc agaaggctcc tcatttacgg aggcctggct gagggaaatg
      181 gagcgccatc tgaaggggat tgcccgaccg gaaggtctgt tcagggatac gctggtcaca
      241 ggtcagccat ggcgtggtca gcagcggcgc acgaggatgg tgatttaccg gtggatcggg
      301 aaaaacaacc acgatccgat gccgccggtg gcaatgctga accaggtctg ttcgcgggtc
      361 gtgggggcgc tgggtggcgc aggcgtccgc tgtacacgga tgaacgggca gcaggtgcat
      421 ggctggctgc tgaggctgtt caatccccgg ccggaatggg tggacaggga tattctgtac
      481 cgtatggcat cccgtgcaga accgcaggag acaccggagg gaatgatgcc ggtgatgacc
      541 gattttgcag aaagcctctg gttcacccca ccggtatctg atccggaaaa cggcgtgtgg
      601 tggctggacg ggcttccgca tgccgctgtt gtggtggaga aactgcgcac accaccggag
      661 ccgggaacca tcacagggga acaggcccgg ggcgaaaaga cagtgaatgc cctgatggac
      721 acctttccgg agggaacggt gctgtgtatg acaattgtgg tccagccaca ggacacactg
      781 gaagagcgct tcacgaggtt gtcgaaaaat gcggtcgg
//
LOCUS       DAZFLD010000620          815 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.623, whole genome shotgun sequence.
ACCESSION   DAZFLD010000620 DAZFLD010000000
VERSION     DAZFLD010000620.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..815
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.623"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..281)
                     /locus_tag="ACHKBL_005320"
     CDS             complement(<1..281)
                     /locus_tag="ACHKBL_005320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5822018.1"
                     /translation="MKVCLYFISVFFSSCCIADVFHYRMPFRIQTYSPVTKSKIENFA
                     IKDGAQKLLQINREKLLMLLNRSSNECYHFDDKNVRIYIIDGKDRYYID"
     gene            complement(339..>815)
                     /locus_tag="ACHKBL_005321"
     CDS             complement(339..>815)
                     /locus_tag="ACHKBL_005321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001600695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HFO5822019.1"
                     /translation="IGLKGGWNFYQYPLNPVSGFDPLGLKVSFQGDESTQKTLKEAYK
                     AVAETKFGHKITEELESSEHEYIFRGLRKGINQTCYDDTEYSFYIDIDNDHSSCVYQG
                     KNKACAMKPTLLSVVLAHEMGHAKGMKDDGTDSMANVDKYENPFRKELGLPARMKY"
BASE COUNT          258 a          166 c          103 g          288 t
ORIGIN      
        1 ttatcaatat aatatctgtc tttaccatca ataatataaa tcctaacatt tttatcatca
       61 aaatggtaac actcattact agacctgttc agtaacatta ataacttctc acgatttatc
      121 tgtaataatt tttgtgcacc atcttttatt gcaaaatttt caattttact tttagtgaca
      181 ggggaatatg tttggatgcg aaaaggcatc ctataatgga aaacatcagc tatacagcaa
      241 gatgaaaaaa atacggaaat aaaatacaga catactttca taccatctcc taaactggtg
      301 atatattaaa aacataccca aaagcaatat aaaaatgttt aatatttcat tcttgctggc
      361 aagcccaact ctttacgaaa tgggttttca tatttatcaa cattagccat cgagtcagtt
      421 ccatcgtctt tcattccctt tgcatgcccc atttcatgcg caagaaccac actcaacaga
      481 gtgggtttca tggcacatgc tttgtttttt ccttgatata cacatgagct atggtcgtta
      541 tcaatatcaa tgtaaaaact atattcagta tcatcgtagc atgtttggtt tatgcctttt
      601 cttaatcctc taaatatata ttcatgctct gatgactcta attcttctgt tattttatgt
      661 ccaaactttg tttctgctac agctttatat gcttctttta aagttttttg tgtactttca
      721 tctccctgaa aagacacttt taaccctaat ggatcgaaac cagataccgg attcaacgga
      781 tactgataaa aattccatcc ccccttcagc ccaat
//
LOCUS       DAZFLD010000621          814 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.624, whole genome shotgun sequence.
ACCESSION   DAZFLD010000621 DAZFLD010000000
VERSION     DAZFLD010000621.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..814
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.624"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..665
                     /locus_tag="ACHKBL_005322"
     CDS             <1..665
                     /locus_tag="ACHKBL_005322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001287261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DotI/IcmL family type IV secretion protein"
                     /protein_id="HFO5822020.1"
                     /translation="PETDNSGDIYKQTLSTIAQSEQRAALVPGLVRALLCSSAALVIS
                     LTGNIIQYWHNSNVTREYFATDNGRLVRLAPTSQPAWSQSDVMTFGSMTLSEAFNLDF
                     VHYRKQITTLAPRFSEAGFAGYVNALQASNILDTIKKEKFNLTATIGAGTLVNQGQME
                     NGIWFWKFQYPVRMRLVGQTSTRPEQAFTFEITVRRVDPRIKPGGMEISQMVSRNAAS
                     GT"
     gene            676..>814
                     /locus_tag="ACHKBL_005323"
     CDS             676..>814
                     /locus_tag="ACHKBL_005323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001165562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraN"
                     /protein_id="HFO5822021.1"
                     /translation="MQIHHLLGALLIVCGTVTAADNPGWQNARTPQAGGKTGQAPGNA
                     PA"
BASE COUNT          202 a          244 c          210 g          158 t
ORIGIN      
        1 ctccggaaac agataacagc ggagatattt ataaacaaac gctcagtacc attgcacagt
       61 cagaacaacg ggccgcactg gttcccggac tggtcagggc cttgctgtgt agcagcgcag
      121 ctctcgtcat cagtctgacc ggcaatatca tacagtactg gcataacagc aacgtaacgc
      181 gtgaatactt tgccaccgac aatggccggc tggtccgcct ggcccccacc agtcagccag
      241 cctggagtca gagcgacgtc atgacgtttg gcagcatgac gctgagcgaa gcttttaatc
      301 tggattttgt ccactacagg aaacaaatca cgacactggc cccccgcttt tccgaagccg
      361 gttttgccgg ctatgtgaat gctcttcagg cctccaacat tctggacacc attaaaaagg
      421 agaaatttaa cctcacggcc accatcgggg ccggcacgct ggtgaatcag ggccagatgg
      481 aaaacggcat ctggttctgg aaattccagt atccggtcag gatgcgtctg gtcggtcaga
      541 ccagcacccg ccccgagcag gccttcacct ttgaaatcac tgtccggcgc gttgacccac
      601 gaatcaaacc cggcggcatg gaaatcagcc agatggtttc ccgtaacgcc gcatcaggaa
      661 cctgagactc atatcatgca aatacatcat cttcttggcg cactgcttat tgtgtgcggc
      721 actgtcacgg ctgcagataa cccgggctgg cagaacgcac gaaccccaca ggctggcgga
      781 aagacaggac aggctcccgg gaacgccccc gcag
//
LOCUS       DAZFLD010000622          814 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.625, whole genome shotgun sequence.
ACCESSION   DAZFLD010000622 DAZFLD010000000
VERSION     DAZFLD010000622.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..814
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.625"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            7..814
                     /locus_tag="ACHKBL_005324"
                     /pseudo
     CDS             7..814
                     /locus_tag="ACHKBL_005324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976766.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          220 a          193 c          227 g          174 t
ORIGIN      
        1 gaacagatga ttgatgtctt agggccggag aaacgcggac ggcgtaccac acaggaaaag
       61 atcgctattg ttcagcagaa ctttgaaccg ggaataactg tctccctcgt tgcacggcaa
      121 cacggtgtga cagccagtta tttctctggc gtaagcaata ccaggaagga aatctcactg
      181 ctgtggctgc tggagaacag gttgttctgg cctctgagct tgctgccgcc atgaagcaca
      241 ttaaagaact ccagcgcctg cccggcaaga aaacgatgga aaattaactc attagagaag
      301 ccgttgaata ttggcgtgca aaaaagtgga tagcgcacgc ccaatattgc ccgggaatgg
      361 ggagtaagtt tcgtcagccg ttatctccgt gtgttgcgtg cgcagttgca cgtcattctc
      421 agacgaaccg atgactggaa ggatggccgc cgcatgatac ggatgtttat ctccgtaata
      481 acatggtgta caccatgtta tcggagagct gcccacgtat ggttatcgtc ggctgtgaaa
      541 cagtacagga cgtcatgctg ggagcggtag aacaccgctt cggcagcgac cttcctgcgt
      601 ctccagtgga gtgcctgacg gataatagtt catgctatca ggctaatgaa acacgccagt
      661 tcgcccggat gttggaactt gaaccgaaga acacggcggt gcggtatccg gagagtaacg
      721 gcatagcaga gagcttcgtg aaaacgataa agcgtgacta catcagtatc atgcccaaac
      781 cagacgggtt aacggcagca aagaaccttg caga
//
LOCUS       DAZFLD010000623          810 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.626, whole genome shotgun sequence.
ACCESSION   DAZFLD010000623 DAZFLD010000000
VERSION     DAZFLD010000623.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..810
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.626"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>810
                     /gene="tssC"
                     /locus_tag="ACHKBL_005325"
     CDS             <1..>810
                     /gene="tssC"
                     /locus_tag="ACHKBL_005325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017892320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
                     /protein_id="HFO5822022.1"
                     /translation="LIDHHIAELDDQISRQLDAVLHHPEFQKVESLWRGVKSLVDKTD
                     FRRNVKIELLDLSKDDLRQDFEDAPEIIQSGLYLQTYVAEYDTPGGEPIAALVSAWEF
                     DASAQDVALLKNISRVAASAHMPFIGSVGPAFFRKETMEEVAAIKDIGNHFERAEYIK
                     WNAFRETDDARYIGLVMPRVLGRLPYGPDTVPVRSFNYVEEVKGPDHHKYLWTNASFA
                     FAANMVRSFVTNGWCVQIRGPQAGGAVQDLPIHLYDLGTGNQVKIPSEVMIP"
BASE COUNT          172 a          185 c          238 g          215 t
ORIGIN      
        1 gctgattgac catcatattg cagagcttga tgaccagatc agtcgtcagc tggatgccgt
       61 gcttcatcat ccggaatttc agaaagtgga atccctgtgg cggggcgtga agtctctggt
      121 ggataaaacg gacttccgcc gtaatgtgaa aattgagctg ctggatctgt ccaaagacga
      181 tctgcgccag gattttgagg atgcgccgga aattattcag agcggccttt atctgcagac
      241 ctatgtggct gaatatgaca cgccgggtgg tgaacccatt gctgccctgg tttcggcctg
      301 ggaatttgat gcatctgcgc aggatgtggc gttgctgaaa aatatttccc gggtggcagc
      361 atccgcacat atgccgttta ttggttctgt tggtccggct ttcttccgga aagaaaccat
      421 ggaagaggtg gctgctatta aggatattgg caatcacttt gagcgtgccg agtacatcaa
      481 gtggaacgct ttccgagaga cggatgatgc ccgctatatt ggtctggtga tgccccgtgt
      541 gctgggacgc ctgccgtatg gccctgatac tgtacctgtc cgcagcttta actatgttga
      601 agaggtgaaa gggccggatc atcacaagta tctctggacg aacgcgtctt ttgcttttgc
      661 ggccaatatg gtgcgcagtt ttgtcaccaa cggctggtgt gttcagatcc gcggtcctca
      721 ggccggtggt gcggtgcagg atttgccgat ccatctgtat gatctgggaa ccggtaatca
      781 ggtcaaaatt ccgtcagagg tgatgatccc
//
LOCUS       DAZFLD010000624          809 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.627, whole genome shotgun sequence.
ACCESSION   DAZFLD010000624 DAZFLD010000000
VERSION     DAZFLD010000624.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..809
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.627"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..360
                     /gene="ssb"
                     /locus_tag="ACHKBL_005326"
     CDS             <1..360
                     /gene="ssb"
                     /locus_tag="ACHKBL_005326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004115562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFO5822023.1"
                     /translation="IAGEYLRKGSQVYIEGQLRTRKWFDQQNGVDRYSTEVVVSVNGT
                     LQMLGSPRQQQGGGNVSSAPAQGGWGQPPQNAQQSTGKNGQASSGSGGMPPMDFDDDI
                     PFMGRGYGVERRIIHVI"
     gene            409..>809
                     /locus_tag="ACHKBL_005327"
     CDS             409..>809
                     /locus_tag="ACHKBL_005327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HFO5822024.1"
                     /translation="MNENTPTTPTPHDAAFRAMMECPSVARDFLKGALPPEQLNRCDM
                     NTLQLEPTTFVDPDLRQFACDVLWSMKTTDGRDGYIYTLTEHQSKADKYMALRMMRYV
                     LAVIHRHLKAHACAPVVIPVLFYHGVPSPYP"
BASE COUNT          196 a          191 c          228 g          194 t
ORIGIN      
        1 attgccggtg aataccttcg taaaggttca caggtgtata ttgaaggtca gttaagaacc
       61 cggaagtggt tcgatcagca gaatggtgtt gatcgttatt ccacggaagt tgttgtgagt
      121 gtcaatggta ccctgcagat gctggggtct ccgcgtcagc agcagggtgg tggaaatgtt
      181 tcatctgctc ctgctcaggg aggatgggga caaccaccac aaaatgcaca acaatcaacg
      241 ggaaaaaacg gtcaggcttc ctcaggaagc gggggaatgc cgccgatgga ctttgacgat
      301 gatatcccct ttatggggcg tgggtatggc gttgaacgac ggattatcca tgtcatctga
      361 tgttaaccac ggggcttacc gccccacttt taattcaagg aggcggttgt gaatgaaaac
      421 acacccacta cccccacgcc acatgatgcg gcgttcagag cgatgatgga atgtccctct
      481 gttgcgaggg atttcctgaa aggcgcgttg cccccggaac aactgaaccg gtgcgatatg
      541 aacaccctgc aactggagcc gaccacgttt gtggatccgg atcttcgcca gtttgcctgt
      601 gatgttctgt ggagtatgaa aaccacagat ggcagggatg gatacattta tacgctgaca
      661 gagcaccaga gcaaggcgga taagtatatg gccctgcgaa tgatgcgcta tgttctggcg
      721 gtgatacatc gccacctgaa agcacatgcg tgtgctcccg ttgtgatacc ggtgcttttt
      781 taccatggtg tacccagtcc ttatccata
//
LOCUS       DAZFLD010000625          809 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.628, whole genome shotgun sequence.
ACCESSION   DAZFLD010000625 DAZFLD010000000
VERSION     DAZFLD010000625.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..809
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.628"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>809
                     /locus_tag="ACHKBL_005328"
     CDS             <1..>809
                     /locus_tag="ACHKBL_005328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6531 domain-containing protein"
                     /protein_id="HFO5822025.1"
                     /translation="VCPGGVTSGHPVNPLLGAKVLPGETDLALPGPLPFILSRTYSSY
                     RTKTPAPVGSLGPGWKMPADIRLQLRDNTLILSDNGGRSLYFEHLFPGEDGYSRSESL
                     WLVRGGVAKLDEGHRLAALWQALPEELRLSPHRYLATNSPQGPWWVLGWCERVPEADE
                     VLPAPLPPYRVLTGLVDRFGRTQTFHREAGGEFSGEITGVTDGAGRHFRLVLTTQAQR
                     AEEARQQAISGGTEPSAFPDTLPGYTEYGRDNGIRLSAVWLTHDPEYPDNLP"
BASE COUNT          135 a          259 c          275 g          140 t
ORIGIN      
        1 gtgtgccccg gcggggtgac gtccggccat ccggtcaatc cgctgctcgg tgcgaaggtc
       61 cttcccggcg aaaccgacct tgccctgccc ggcccgctgc cgttcatcct ctcccgcacc
      121 tacagcagtt accggacaaa aacgcccgcg ccggtgggga gcctcggccc cggctggaaa
      181 atgcctgcgg atatccgctt acagctgcgc gataacacac tgatactcag tgataacggc
      241 ggcagaagcc tgtattttga gcacctgttt cccggtgagg acggttacag ccgcagcgag
      301 tcactctggc tggtgcgcgg cggcgtggcg aaactggatg aaggtcatcg gctggccgca
      361 ctctggcagg cgctgccgga agagcttcgc ctgagtccgc atcgttatct ggcgacgaac
      421 agtccgcagg ggccgtggtg ggtactcggc tggtgtgagc gggtgccgga ggcggatgag
      481 gtgctgcctg cgccgctgcc gccgtaccgg gtactgaccg ggctggtgga ccgcttcggg
      541 cgcacacaga cgttccaccg tgaggctggc ggcgaattca gcggcgaaat caccggcgtg
      601 acggatggtg ccgggcgtca cttccggctg gtactgacca cgcaggcgca gcgggcagaa
      661 gaggcccggc agcaggccat ttccggcggg acggaaccgt ccgcttttcc tgataccctg
      721 ccgggttaca ccgaatatgg ccgggacaac ggcatccgtc tgtctgccgt gtggctgacg
      781 cacgacccgg aatatccgga caacctgcc
//
LOCUS       DAZFLD010000626          809 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.629, whole genome shotgun sequence.
ACCESSION   DAZFLD010000626 DAZFLD010000000
VERSION     DAZFLD010000626.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..809
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.629"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..290
                     /locus_tag="ACHKBL_005329"
     CDS             <1..290
                     /locus_tag="ACHKBL_005329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001544930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO5822026.1"
                     /translation="VGKTFPEMQKILPIFLKPLYFISCIMFPLHSIPQQYWTYLLWNP
                     LVHVVELSREAVMPGYISEGVSLNYLAMFTLVTLFIGLALYRTREEAMLTS"
     gene            287..>809
                     /locus_tag="ACHKBL_005330"
     CDS             287..>809
                     /locus_tag="ACHKBL_005330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001605887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO5822027.1"
                     /translation="MIKIENLTKSYRTSTGRHYVFKDLNIELPSGKSVAFIGRNGAGK
                     STLLRMIGGIDRPDRGKIITNKTISWPIGLAGGFQGSLTGRENVKFVARLYAKPEELK
                     DKIEFVEEFAELGKYFDMPIKTYSSGMRSRLGFGLSMAFKFDYYIVDEVTAVGDARFK
                     EKCAHYFKERHKES"
BASE COUNT          235 a          158 c          185 g          231 t
ORIGIN      
        1 tcgttggtaa aactttccct gaaatgcaaa agattctacc gatatttctt aaaccgctgt
       61 atttcatttc ctgtatcatg ttccctctac attcgatccc acagcaatac tggacatatc
      121 tactctggaa cccattagtc catgtcgtag agttaagtcg tgaggcagtt atgccgggct
      181 atatcagcga aggcgtgagt ttgaactatc tggcaatgtt cactctggtc accctgttca
      241 tcggtctggc gctataccga acgcgtgaag aggcaatgct gacatcatga ttaagattga
      301 gaatctgacg aagtcatatc gcacgtcaac aggtcgacac tatgtgttta aggatttaaa
      361 tatcgaactc ccttcaggaa aaagtgttgc ctttattggt cgtaatggtg caggtaaatc
      421 aactttactg agaatgattg gcggtattga ccgccccgat agaggaaaga tcatcaccaa
      481 taaaacgata tcatggccca ttgggctcgc cggtggattc cagggcagtt taaccgggcg
      541 agaaaacgta aaatttgttg cacggttata tgcgaagcca gaagaactga aagataaaat
      601 tgagtttgtc gaagaatttg ccgaactcgg taagtacttt gatatgccga tcaaaactta
      661 ctcatctggt atgcgatctc gcctgggttt tggtttaagc atggcattta agtttgatta
      721 ttatattgtc gatgaagtaa cggcagtcgg tgatgccagg tttaaagaaa aatgtgcaca
      781 ttactttaaa gaaaggcata aagagtcta
//
LOCUS       DAZFLD010000627          808 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.630, whole genome shotgun sequence.
ACCESSION   DAZFLD010000627 DAZFLD010000000
VERSION     DAZFLD010000627.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..808
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.630"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..779)
                     /locus_tag="ACHKBL_005331"
     CDS             complement(<1..779)
                     /locus_tag="ACHKBL_005331"
                     /inference="COORDINATES: protein motif:HMM:NF017826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF927 domain-containing protein"
                     /protein_id="HFO5822028.1"
                     /translation="MLKLSWDATRYGLTVEAAARNDGFIPIDEIGQGGRINDIAQSAY
                     SLFNGVGRIQGRKDGGNRAVIRWRIAALSTGEEDFETFLIKGGVTPKAGQLVRLLSVP
                     FVDTTVFNGYDDGDQHARAIKRFASNYCGAAGREWIGWLSANKELAVSVVNEKENIWL
                     SRLPENASPQVKRVASRFAMLDAAGELATGITGWTAGESHVATQRAFDDWLQDFGLDN
                     REKYQVISRARDFIQRHALSRFQPYTYGRQNGDMDVNYGAR"
BASE COUNT          182 a          242 c          177 g          207 t
ORIGIN      
        1 attctcgctc cgtaattcac gtccatatct ccgttctgtc tgccgtatgt gtacggctga
       61 aaacggctaa gggcgtgacg ctgaatgaaa tcgcgggcgc gactgataac ctgatatttt
      121 tccctgttat ccagtccaaa atcctgcaac cagtcatcaa aagcgcgctg tgtggctaca
      181 tggctttctc ccgctgtcca tccggttatt cctgttgcca gttctccggc tgcatccagc
      241 attgcaaaac gggatgccac gcgtttcacc tggggggaag cattttcagg aagacgacta
      301 agccagatgt tttctttttc gttaaccaca ctgaccgcaa gttctttgtt ggctgacaac
      361 cagccgatcc actcacgacc cgctgctccg caataattcg atgcaaaccg tttgattgct
      421 ctggcgtgct gatcaccatc gtcatatccg ttaaaaaccg ttgtatcaac aaacggcaca
      481 cttaacaggc gcaccagttg accagcttta ggcgtcacac cgcccttaat caggaaggtt
      541 tcgaaatcct cctctcccgt gctcagggcc gcgattctcc agcgtatcac cgcacggtta
      601 ccgccatcct tacgcccctg aatacgcccc acaccgttaa acaggctgta ggctgactgc
      661 gcaatatcgt taatccgtcc gccctgaccg atttcatcaa tggggataaa gccatcatta
      721 cgtgccgcag cttccaccgt gaggccgtat ctggttgcat cccatgaaag tttcagcatt
      781 tcaggatcac cgtaaagact tgttgctg
//
LOCUS       DAZFLD010000628          803 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.631, whole genome shotgun sequence.
ACCESSION   DAZFLD010000628 DAZFLD010000000
VERSION     DAZFLD010000628.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..803
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.631"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(152..>803)
                     /locus_tag="ACHKBL_005332"
     CDS             complement(152..>803)
                     /locus_tag="ACHKBL_005332"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5822029.1"
                     /translation="HIEELEAYLLVSGLADSRTRGLISYELTPQEYNAIELLNSNNLM
                     WGTYNLKKLFGPAIRGKRLKNADRIPGSLPFVTAGEGNEGISAFISNDVVVFPENTIT
                     IDMFGSAKYRNYEYGADDHVAVVHTEFFDRNAVIFITASIHKSSCTGKFNYGRNFYAK
                     DADELMVSLPSKDNNPDYSFMETLILAVHKLVIKDVVQYTDKKLVAYRQITSEKVV"
BASE COUNT          225 a          179 c          157 g          242 t
ORIGIN      
        1 gatacaaaaa acccgcgcat cggcgggtta agcagcgtgg caatgtaacc acttttgtca
       61 tgatatgatg atttttacga ttgtaaactg tttttctgca tcagggcacg ataacctgac
      121 cattccccac cctgtcaggt ggggatttgc actaaacaac cttctcagat gtaatctgcc
      181 tgtacgctac cagcttttta tcagtgtact ggacaacatc tttgatgacc agtttatgta
      241 ctgctagaat tagcgtctcc atgaaagaat aatcagggtt attatctttt gacggcaaag
      301 ataccattaa ctcgtcggca tcttttgcat agaaatttct cccgtagttg aactttccgg
      361 tacaagaaga cttatgaatt gatgctgtta tgaaaataac ggcatttctg tcaaagaatt
      421 ctgtatgaac aactgccaca tggtcatctg caccatactc ataatttctg tattttgcgg
      481 agccaaacat atctatcgta atggtatttt cagggaatac aacaacatca ttactgataa
      541 atgcagaaat accttcattg ccttcacccg cagtaacaaa aggcaaggag ccgggtatac
      601 gatctgcgtt tttcagacgc ttacctctta ttgcggggcc aaataacttt ttgaggttat
      661 aagtgcccca cataagatta ttgctattta atagctcaat cgcgttatat tcctgtggtg
      721 taagctcata gcttatcagt ccgcgagtcc gcgagtccgc gagtccgcta accagcaagt
      781 aagcttccag ctcttctata tgg
//
LOCUS       DAZFLD010000629          794 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.632, whole genome shotgun sequence.
ACCESSION   DAZFLD010000629 DAZFLD010000000
VERSION     DAZFLD010000629.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..794
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.632"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>794
                     /locus_tag="ACHKBL_005333"
     CDS             <1..>794
                     /locus_tag="ACHKBL_005333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HFO5822030.1"
                     /translation="KSLTGFTLPVVLSAEFSGPETPWIIVRGDKPVVCPVNHAPQAFT
                     DWLQVEDNILALPVISEAFSFIRNTLADELEKADRLMPPVRAFSVVMRLGAVLSGTPS
                     VWSDRLCSRTCLQFSRKPEQTVPAGMFPDAVLSLLAPFASTVQGGQRTRRLILLIWLC
                     VLTALGISALNNRDLIRQVSTDLQRWNAVPKGHYRPEAESLAALKQDALLLEQWQRQG
                     VPLRYSLGYYPGQRLWLALQQAIDTRVPPPSPEPEPVPEIVRLDSLA"
BASE COUNT          149 a          219 c          234 g          192 t
ORIGIN      
        1 aaatccctga cgggtttcac actgccggtg gtgttgagtg ccgaattttc cgggcctgaa
       61 acaccgtgga taatcgtccg gggggataag ccagttgtat gtccggttaa tcatgcacct
      121 caggcattca ctgactggct gcaggtggaa gacaatattc tggcactgcc ggtcatcagc
      181 gaggcatttt cctttatccg taatacgctg gctgatgagc tggaaaaagc tgaccggctg
      241 atgccacctg tacgggcatt ttccgttgta atgcgccttg gtgccgtcct ttcggggact
      301 ccgtcagtct ggtccgacag gctctgttcc cgcacctgtt tgcagttttc ccgaaaaccg
      361 gagcagacag tgccggcagg gatgttcccg gatgcggttc tgtcgctgct ggcacctttt
      421 gcgtcgacgg tacagggagg ccagcgtacc cgtcgtctga ttttgctgat atggctgtgt
      481 gtcctgactg cgctggggat ttctgcgctt aacaatcgtg atctgatccg ccaggtaagc
      541 accgatttac agcgctggaa tgctgtcccg aaggggcact accgtcctga ggcggaatcg
      601 ctggccgcac tgaaacagga tgcgcttctg cttgagcagt ggcagcgtca gggagtgcct
      661 ttacgttaca gcctgggcta ttacccggga caacgtctgt ggctggccct gcagcaggct
      721 atcgataccc gggttccacc accatcccct gaaccagaac cggtgccgga aattgtacga
      781 ctcgatagcc tggc
//
LOCUS       DAZFLD010000630          793 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.633, whole genome shotgun sequence.
ACCESSION   DAZFLD010000630 DAZFLD010000000
VERSION     DAZFLD010000630.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..793
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.633"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            42..242
                     /locus_tag="ACHKBL_005334"
                     /pseudo
     CDS             42..242
                     /locus_tag="ACHKBL_005334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308358.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase"
BASE COUNT          262 a          169 c          147 g          215 t
ORIGIN      
        1 aatatacaca tcaaaatcag ataaacattt gaggtattgt catgacaaat aaacacctat
       61 cactgttttc tccttttatg ctgacagaaa aaataaagcc atgtaatcgt atcgttatgg
      121 ctcctatgac gacctggtca gctaatcctg atggaaccat ctctgagcaa gaactcgaat
      181 attataaacg tcgctcacaa aatattggtt tagtaattac aggatatacc tatgtcacgc
      241 caaggaaatt cagacatcga aaggaactgg ctggtttccg ttaatggatt aaatatcgat
      301 tactctcccg agagtaaaac tatcaaatca atgagtaacc gatacagcct cgcaaattct
      361 ttcagataat taacccgtca ctgcggcacg ttctctcgta aggcgtgcct gcagtgtaat
      421 ccagtatgta tccggcatag ttgacggttt aatagactca attcctctcc ctggttacct
      481 acaacgatat cttgtataac accccaggag atacgacgtt acgatgagat gcaatatgct
      541 cctcagcgcc aatcaacgcc aggactgggc cagtcttgag atcagactca ttcccgtaac
      601 tgatttcagg cgacatatat caatgacgtt ggggcaataa aagatagccg atgtaataca
      661 tctattcaaa tttgtgatac atgtcaaaaa attggttgac caaactcgtt atttatataa
      721 gggcacttac gaagtgcact cttttttaaa gcgaggaagt accaatgaaa gagaataaag
      781 tacagcaaat cag
//
LOCUS       DAZFLD010000631          792 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.634, whole genome shotgun sequence.
ACCESSION   DAZFLD010000631 DAZFLD010000000
VERSION     DAZFLD010000631.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..792
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.634"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>792
                     /locus_tag="ACHKBL_005335"
     CDS             <1..>792
                     /locus_tag="ACHKBL_005335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HFO5822031.1"
                     /translation="ASAFTTGADGTYSESAGASENSLRWGVGKPLYQDLVSRTKAALA
                     KNPKNRLLAVVWMQGEGDAAVGTHAQHPGLFSAMVNQFRTELAGQASQSTGGSASAVP
                     WVCGDTTYFWKQRHAEGYASVYGGYKGKESQNIFFVPLMTDENGANVQTNDPAEDPDL
                     EAVGYYGSKWRNDQKTWTSVDRASHFSSWARRGIISDRLATAILVHAGRTAEFITGKQ
                     PDTVKPTGPSGEGPEREPEAPVSNRTLMSLLASGEDLASQGWRYY"
BASE COUNT          188 a          187 c          263 g          154 t
ORIGIN      
        1 gtgcttcggc attcacaacg ggtgcagacg gcacatacag cgaatcagcc ggtgcatcgg
       61 aaaattcact gcgctggggt gtgggtaagc cgctgtatca ggatttggtg agccggacta
      121 aagccgcact ggcgaagaac ccgaaaaacc gtctgcttgc ggtggtgtgg atgcagggag
      181 aaggtgatgc ggcagtgggg acgcatgcgc agcatccggg gctgtttagt gcgatggtga
      241 atcagttcag aacggagctt gccggtcagg catcacagag cacaggaggc agcgcatcag
      301 cggtaccgtg ggtttgcggg gacacaacgt atttctggaa gcagcgtcat gcggaagggt
      361 atgcatcagt atacggaggg tataagggta aggagtcgca gaacattttc tttgtaccgt
      421 taatgacgga tgagaatggt gcgaatgtgc agaccaatga cccggcagaa gacccggacc
      481 tggaagccgt cggttattac ggttcaaagt ggcgtaatga ccagaagacc tggacatctg
      541 tggacagggc cagccatttc agttcatggg ctcgccgtgg gattatttcc gaccgtctgg
      601 caacggcgat tctggtgcat gccgggagaa ccgctgaatt cattaccgga aaacagcctg
      661 atacggtgaa gcccaccgga ccttccggtg aaggcccgga gagagagccg gaagccccgg
      721 tcagtaaccg aaccctgatg agtctgctgg cgtccggtga agacctggca tcacagggct
      781 ggcgctatta tc
//
LOCUS       DAZFLD010000632          791 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.635, whole genome shotgun sequence.
ACCESSION   DAZFLD010000632 DAZFLD010000000
VERSION     DAZFLD010000632.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..791
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.635"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
BASE COUNT          193 a          118 c          169 g          311 t
ORIGIN      
        1 acatccaata caaaagtgta tgacctgaag aggtctggag ctatgttgat ttcctgttca
       61 aaaaagaatt aaggttaatt agtattatta gtgggagttt tgttgtgttc ttattatgct
      121 ttattatgct atcttgagtc tttatgttgt aatgtctgct cgccactgta tgctagctgt
      181 acactttact ctgtaagtat gtgtacagca ttatctccat actactggtg tgagagttta
      241 cctgtgtggt gaattatact ctttgagagt accataaatg ttgagaatgt taatcctgag
      301 tatgtgtctg gtgcatagtt agtgttaagt gtgtactaaa acgctattag tgtgttatta
      361 ctctattgag gttcggtggt attaataaat attatctttt tgtatgggct aatcatctct
      421 ggtgtgggcg tatctctcat atcgttattc tttatgctac tggtcaaata ttggtgaggc
      481 ttcctcacag agtttctgtt tttacttaat ggcaggataa tattaatagc gatgttacct
      541 gaggctacct ggtactgact tttgtgaatg tcaggttgag tgaatggtgt tttcttaaac
      601 tcaattctat gaggtggtgt ttttaccatt gtaatttgta ctggtcctca gtttaagaga
      661 gtctcactgt attctgaaat attacctcat tgctgtaatg aggtaataca aaacagtttt
      721 atcaggttgt ctgtgctgct ggttatctct tttattaatg ccgtagtacg acgtttctgt
      781 accctcagca t
//
LOCUS       DAZFLD010000633          789 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.636, whole genome shotgun sequence.
ACCESSION   DAZFLD010000633 DAZFLD010000000
VERSION     DAZFLD010000633.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..789
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.636"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..633
                     /locus_tag="ACHKBL_005336"
     CDS             <1..633
                     /locus_tag="ACHKBL_005336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPASM domain-containing protein"
                     /protein_id="HFO5822032.1"
                     /translation="KSIGSKHMQFIELLETGTPNIDFSGHTENTFRIIDFSVPPTAYG
                     KFMSTIFMQWVKNDVGEIFIRQFESFVSRFLGNGHTSCIFQESCKDNLVVESNGDIYE
                     CDHFVYPQYKIGNINKSELKTMNSVQLTAQKKRISAKCQQCVYKPICNGGCPKHRITK
                     VNNETISYFCEGYKILFSTMVPYMNAMVELAKNRVPLYHIMDVAKQMENN"
     gene            complement(599..721)
                     /locus_tag="ACHKBL_005337"
     CDS             complement(599..721)
                     /locus_tag="ACHKBL_005337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5822033.1"
                     /translation="MTKHPTGIYVGCFVKVIRRRLRLEQRRSVINYSPFVLQHP"
BASE COUNT          236 a          158 c          156 g          239 t
ORIGIN      
        1 aaatctatcg gcagtaaaca tatgcaattt atcgaattgc tcgaaaccgg gacgccgaat
       61 attgatttca gtggtcatac tgagaataca ttccgtatca ttgatttttc tgtgcctccc
      121 acggcttatg gcaagtttat gtcaaccatt tttatgcaat gggttaaaaa cgatgtgggt
      181 gaaattttca tccgtcagtt tgaaagcttt gtcagccgtt ttttggggaa tgggcatacc
      241 agttgtattt tccaggagtc ctgcaaggat aatctggttg ttgaaagtaa tggagacatt
      301 tacgaatgcg accattttgt ctatccacag tacaaaattg gaaacattaa taaatcggaa
      361 ctcaaaacga tgaacagtgt acaactgaca gcgcaaaaaa aacggatttc agcgaaatgt
      421 cagcaatgtg tatataaacc tatctgcaat ggtggttgcc ctaagcatcg tattactaaa
      481 gtaaacaatg agactatttc ctatttttgc gaaggttata aaatcctttt ttcaaccatg
      541 gtaccttata tgaacgccat ggtggagtta gccaagaaca gagtaccgct ttaccacatt
      601 atggatgttg caaaacaaat ggagaataat taataacgct ccttctttgc tccagtctta
      661 gcctccgtct tatgacctta acaaagcacc cgacataaat tcctgttggg tgtttcgtca
      721 tcatcatctt cccttgcgaa aattctggct tacgcggcat gcgtcagggc attagcaata
      781 cccttgatt
//
LOCUS       DAZFLD010000634          788 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.637, whole genome shotgun sequence.
ACCESSION   DAZFLD010000634 DAZFLD010000000
VERSION     DAZFLD010000634.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..788
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.637"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..621)
                     /locus_tag="ACHKBL_005338"
     CDS             complement(<1..621)
                     /locus_tag="ACHKBL_005338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HFO5822034.1"
                     /translation="MATMTDHQKEFISLFNRTACYHKRHKVFEDFVSCSVIALENRLQ
                     FSEAREQKYLRIVSGYEKQDVIHIAHLLAHVIEGLEQGFCDFLGSVFMQLELGDKYRS
                     QFFTPWSIASMMAQMQMGDVKALFENKPFITLSEPACGAGSMILAMADILNRSGYPAY
                     HRMWVSATDIDPLAAGMAYIQLSLCGVAGEVVIGNSLCNERRRVLLT"
BASE COUNT          206 a          222 c          180 g          180 t
ORIGIN      
        1 agtcagcagt acgcggcgtc gctcattaca gagcgagtta ccaatcacca cttctccggc
       61 cacaccacac aacgacagtt gtatatacgc catgccggct gccagcgggt caatatccgt
      121 tgccgacacc cacatcctgt ggtatgccgg ataccccgaa cggttcagga tgtcagccat
      181 tgccagaatc atactgccgg caccacaggc aggttcactg agcgtgataa agggtttgtt
      241 ctcaaacaac gccttcacat ctcccatctg catctgtgcc atcatgctgg caatactcca
      301 gggcgtgaaa aactgactcc ggtatttatc ccccagttcc agttgcatga acacactccc
      361 cagaaaatca cagaatccct gctccagtcc ctcaatgaca tgagcaagca aatgcgcaat
      421 gtggataaca tcctgctttt cataaccact gacgattcgc agatacttct gttcccgggc
      481 ctcactgaac tgaagccggt tttccagggc aatcacacta cagctgacaa agtcttcaaa
      541 caccttatga cgtttgtgat aacaggctgt ccggttaaac aacgaaatga actccttctg
      601 atggtcagtc atggtggcca tgttttctcc gtaaaaacag ggaacacatg gcccggacag
      661 gggaacaggt gttccccttc gggttgaaat gttgataaaa cgggcatcac ggacatgata
      721 cccggaggtt aattactggg ctgagaccag aggaaccggt aaaggtgcgg ctttcgtctg
      781 ttcttcag
//
LOCUS       DAZFLD010000635          787 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.638, whole genome shotgun sequence.
ACCESSION   DAZFLD010000635 DAZFLD010000000
VERSION     DAZFLD010000635.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..787
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.638"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..144)
                     /locus_tag="ACHKBL_005339"
     CDS             complement(<1..144)
                     /locus_tag="ACHKBL_005339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component-system connector protein AriR"
                     /protein_id="HFO5822035.1"
                     /translation="MLEDTTIHNAIADKALSNYFRSSGNLLEEESAVLGQAVTNLMLS
                     GDNV"
     gene            complement(173..445)
                     /gene="ymgA"
                     /locus_tag="ACHKBL_005340"
     CDS             complement(173..445)
                     /gene="ymgA"
                     /locus_tag="ACHKBL_005340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component-system connector protein YmgA"
                     /protein_id="HFO5822036.1"
                     /translation="MKTSDNESTKYAITGQAVLQILRMKINFSLQTLIKQLLVMKSVE
                     ENAFRRDLIDSIIRDFSNSDTGGPNRRTAITDNKSIFNGKKINRIH"
     gene            complement(488..724)
                     /gene="ycgZ"
                     /locus_tag="ACHKBL_005341"
     CDS             complement(488..724)
                     /gene="ycgZ"
                     /locus_tag="ACHKBL_005341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein YcgZ"
                     /protein_id="HFO5822037.1"
                     /translation="MHQNSVTLDSAGAITRYFAKANLPTQQETLGEIVTEILKDGRIL
                     SRKSLCAKLLCRLEQATGEEEQKHYNALIGLLFE"
BASE COUNT          229 a          167 c          127 g          264 t
ORIGIN      
        1 aacattatca cctgaaagca ttaaattggt gacagcctgc cctaacacag ctgactcttc
       61 ttctaacaaa ttacccgaac tgcgaaagta atttgataac gctttatcag ctattgcatt
      121 atgaatagta gtatcttcaa gcatagtaac ctctcatctt acttatgaaa ttttaatgta
      181 ttctgtttat tttcttacca ttgaaaatac ttttattgtc agttatcgct gttcttctgt
      241 ttggccctcc tgtatcactg ttgctaaaat cacgaattat gctgtcaatt aaatctcgtc
      301 ggaaagcatt ttcctcaaca gatttcatta cgagtaattg cttaataaga gtctgcaacg
      361 aaaaatttat cttcatgcgc agtatctgga gcaccgcctg gccagtaatt gcatattttg
      421 tactttcatt atcagatgtc ttcataacga cttcatagct aacctgttaa ataatcaggc
      481 tgttgcatta ttcaaaaagc aacccaatta gtgcattata atgtttctgt tcctcttccc
      541 ctgtcgcttg ttccagtcgg catagaagtt tggcacaaag cgactttcga ctcagaatac
      601 gtccatcttt caaaatctca gtcacaattt ccccgagagt ttcctgctga gtaggcaagt
      661 tagcttttgc aaagtaacga gtgattgctc ccgcagaatc taaagtcact gaattttgat
      721 gcatgctacg cctctgttaa aaatgttaat caatgttaag tgaggtaaca atagcaaact
      781 tgtacga
//
LOCUS       DAZFLD010000636          781 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.639, whole genome shotgun sequence.
ACCESSION   DAZFLD010000636 DAZFLD010000000
VERSION     DAZFLD010000636.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..781
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.639"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..263)
                     /gene="ybfB"
                     /locus_tag="ACHKBL_005342"
     CDS             complement(<1..263)
                     /gene="ybfB"
                     /locus_tag="ACHKBL_005342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbfB"
                     /protein_id="HFO5822038.1"
                     /translation="MKCIIFLFRAIWLALSLLILFFSMHRLSLLDSTRDVSELISLMS
                     YGMMVICFPTGIVFFIALIFIGTVSDIIGVRIDSKYIMAIIIWL"
     gene            complement(263..>781)
                     /locus_tag="ACHKBL_005343"
     CDS             complement(263..>781)
                     /locus_tag="ACHKBL_005343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HFO5822039.1"
                     /translation="KGGWNFYQYPLNPITNIDPLGLETLKCIKPLHSMGGTGERSGPD
                     IWGNPFYHQYLCVPDGKGDYTCGGQDQRGESKGDGLWGPGKASNDTKEAAGRCDLVET
                     DNSCVENCLKGKFKEVRPRYSVLPDIFTPINLGLFKNCQDWSNDSLETCKMKCSGNNI
                     GRFIRFVFTGVM"
BASE COUNT          250 a          180 c          115 g          236 t
ORIGIN      
        1 agccatatta ttatcgccat aatatactta ctatcaatcc ttacgccaat aatgtctgaa
       61 acagtcccta taaatattag agcaataaag aatactatgc ctgttggaaa acatattacc
      121 atcattccat aagacatgag actaattaac tcgctcacat cacgagtgga gtctaatagt
      181 gaaagtctat gcatggaaaa gaatagtatt aaaagcgata aagccagcca tatagctctg
      241 aataaaaaaa taatacattt cattacatca ctccggtaaa tacaaatcta ataaaacgtc
      301 caatgttatt tccggagcac ttcatcttac atgtttctaa agaatcatta gaccagtctt
      361 ggcagttttt aaatagccct aaatttatag gtgtgaatat atcaggcaat acagaataac
      421 gcggccttac ctctttaaac ttccctttta aacagttctc cacacaacta ttatcggttt
      481 caacgaggtc acaacggcca gcagcttctt ttgtatcatt acttgcttta cctggccccc
      541 atagaccatc tccttttgat tctccccgtt ggtcttggcc accacaagta tagtcccctt
      601 taccatctgg gacacaaaga tattgatgat agaacggatt cccccatata tctggaccgc
      661 ttctttcacc agttccgccc attgaatgca gtggcttaat gcattttagt gtttctaatc
      721 ctaaaggatc tatattagtg atcggattca acggatactg ataaaaattc catcccccct
      781 t
//
LOCUS       DAZFLD010000637          781 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.640, whole genome shotgun sequence.
ACCESSION   DAZFLD010000637 DAZFLD010000000
VERSION     DAZFLD010000637.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..781
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.640"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            174..644
                     /locus_tag="ACHKBL_005344"
     CDS             174..644
                     /locus_tag="ACHKBL_005344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Panacea domain-containing protein"
                     /protein_id="HFO5822040.1"
                     /translation="MAYSAAAVANAFIDKAKRGEIKDLTPMKLQKLLFYTQSWHLKLF
                     NGRPLFDDLFARWPYGPVIPHLYHMLKKYGGDEIQEMISIIIDSDGEWKRITPTIQKG
                     DTETRKLIDRIVEVYGSYSGTELSNMTHAPNAAWSKGQPDGGPILHTDMANHIH"
BASE COUNT          207 a          187 c          165 g          222 t
ORIGIN      
        1 cagtagctta tctgctttct ccatagtcgt ccaactcacc ggactttctt cacctcgtga
       61 ctgccgttca cctctaattt ttgtttcctg ccctctctat cagttttgct aatttgcttt
      121 ttgttgtatt ctctccggtg atacatttaa tttcgcaaaa tgcggagtag agcatggcat
      181 attcagctgc tgccgtagct aacgccttta ttgataaggc taaacgggga gaaatcaaag
      241 atttaactcc gatgaagcta caaaaattgt tgttctatac tcagtcgtgg catctgaaac
      301 tattcaacgg aaggccatta ttcgacgatc tgtttgcgcg ctggccttac gggcctgtaa
      361 taccgcacct gtaccacatg ttaaagaaat atgggggcga tgaaatacag gaaatgatca
      421 gcatcatcat tgattcagat ggggaatgga aacgaatcac accgacaata cagaagggtg
      481 acacagaaac caggaagctg atcgacagaa ttgttgaggt atacggcagc tacagcggaa
      541 ctgagctttc taacatgacc catgcaccaa acgcggcctg gagtaaaggt caacctgatg
      601 gtggcccaat tcttcatact gatatggcaa accacattca ttgatgtgtg aacttgaaaa
      661 aatctccatc tctgaatctg ttcgctgctt ttcttggtgt tatcagggag cttttccctc
      721 acgccaaatc tcacacagag taaccggcct cagcgccggt ttttctttgc gcatcctcca
      781 t
//
LOCUS       DAZFLD010000638          781 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.641, whole genome shotgun sequence.
ACCESSION   DAZFLD010000638 DAZFLD010000000
VERSION     DAZFLD010000638.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..781
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.641"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..768
                     /gene="ompT"
                     /locus_tag="ACHKBL_005345"
     CDS             <1..768
                     /gene="ompT"
                     /locus_tag="ACHKBL_005345"
                     /EC_number="3.4.23.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="omptin family outer membrane protease OmpT"
                     /protein_id="HFO5822041.1"
                     /translation="DWKFNSAAIIKGAINWDLMPQISIGAAGWTTLGSRGGNMVDQDW
                     MDSSNPGTWTDESRHPDTQLNYANEFDLNIKGWLLNEPNYRLGLMAGYQESRYSFTAR
                     GGSYIYSSEEGFRDDIGSFPNGERAIGYKQRFKMPYIGLTGSYRYEDFELGGTFKYSG
                     WVESSDNDEHYDPGKRITYRSKVKDQNYYSVAVNAGYYVTPNAKVYVEGAWNRVTNKK
                     GNTSLYDHNNNTSDYSKNGAGIENYNFITTAGLKYTF"
BASE COUNT          251 a          162 c          175 g          193 t
ORIGIN      
        1 gactggaaat tcaatagcgc tgcaattatt aaaggtgcaa ttaattggga tttgatgccc
       61 cagatatcta tcggggctgc tggctggaca actctcggca gccgaggtgg caatatggtc
      121 gatcaggact ggatggattc cagtaacccc ggaacctgga cggatgaaag tagacaccct
      181 gatacacaac tcaattatgc caacgaattt gatctgaata tcaaaggctg gctcctcaac
      241 gaacccaatt accgcctggg actcatggcc ggatatcagg aaagccgtta tagctttaca
      301 gccagaggtg gttcctatat ctacagttct gaggagggat tcagagatga tatcggctcc
      361 ttcccgaatg gagaaagggc aatcggctac aaacaacgtt ttaaaatgcc ctacattggc
      421 ttgactggaa gttatcgtta tgaagatttt gaactcggtg gcacatttaa atacagcggc
      481 tgggtggaat catctgataa cgatgaacac tatgacccgg gaaaaagaat cacttatcgc
      541 agtaaggtca aagaccaaaa ttactattct gttgcagtca atgcaggtta ttacgtcaca
      601 cctaacgcaa aagtttatgt tgaaggcgca tggaatcggg ttacgaataa aaaaggtaat
      661 acttcacttt atgatcacaa taataacact tcagactaca gcaaaaatgg agcaggtata
      721 gaaaactata acttcatcac tactgctggt cttaagtaca cattttaaga acgccaacta
      781 a
//
LOCUS       DAZFLD010000639          780 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.642, whole genome shotgun sequence.
ACCESSION   DAZFLD010000639 DAZFLD010000000
VERSION     DAZFLD010000639.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..780
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.642"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..333)
                     /locus_tag="ACHKBL_005346"
     CDS             complement(<1..333)
                     /locus_tag="ACHKBL_005346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001298206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFO5822042.1"
                     /translation="MRQYRLLSPMIKVVIVLFSLLFSTAQAGLVIGGTRFAYPENQSS
                     ISVELKNTSDRDMLVKVAVSPDDARQLTGTVESLPSFSGTVFIATPPLFVLKPDKNTK
                     IRINRVGGS"
     gene            complement(299..>780)
                     /locus_tag="ACHKBL_005347"
     CDS             complement(299..>780)
                     /locus_tag="ACHKBL_005347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO5822043.1"
                     /translation="STNSFADDITHAGVVRIEGLITEKTCIISDESKNFTVNMPDVPS
                     SSVRSAGDVTEKVYFSITLTRCGSDVGNAYIKFTGNTVSEDASLYKLEDGSVDGLALT
                     IFDKNKGSISNDVKSIGFSLTSSVDNILHFFAAYKALKNNVQPGDANASVSFIVTYD"
BASE COUNT          246 a          167 c          138 g          229 t
ORIGIN      
        1 actgccacca acacgattaa ttcttatttt tgtatttttg tcaggcttca aaacaaacag
       61 tggcggagtt gcgataaata ccgttccaga aaatgagggc aaagattcaa ccgttcccgt
      121 caactgacga gcatcatcag gggagactgc aaccttgact aacatatctc tgtctgaagt
      181 gtttttgagt tcaacagata ttgaggactg attttccgga tacgcaaaac gcgttccacc
      241 gatcactaat cctgcttgtg ctgtagagaa taaaagggaa aataatacta tgactacctt
      301 aatcataggt gacaataaac gatactgacg cattggcatc ccctggttgg acattatttt
      361 ttaatgcttt gtaagccgca aaaaaatgca atatattatc aactgatgat gtaagtgaaa
      421 aacctatgct tttaacatca ttactaatac tgcctttgtt cttatcaaaa atcgtgagtg
      481 caagcccatc taccgagcca tcttccagct tatataaact ggcatcttca gaaactgtat
      541 tgccggtaaa ctttatatac gcgttgccaa catcactacc acagcgggtt aacgttatgg
      601 aaaaataaac cttttcagta acatcccctg cactccttac cgaactactg ggtacgtctg
      661 gcatattaac tgtaaaattt tttgactcat cagaaataat gcaggttttt tcggtaatta
      721 gcccttcaat acgaaccact ccggcatgag tgatatcatc agcaaaagaa tttgttgata
//
LOCUS       DAZFLD010000640          776 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.643, whole genome shotgun sequence.
ACCESSION   DAZFLD010000640 DAZFLD010000000
VERSION     DAZFLD010000640.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..776
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.643"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(204..>776)
                     /locus_tag="ACHKBL_005348"
     CDS             complement(204..>776)
                     /locus_tag="ACHKBL_005348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HFO5822044.1"
                     /translation="MLRLRRAGQITGQHVPEIILLNSHDGSSSYQMLPGYFRAICTNG
                     LVCGQSLGEVRVPHRGNVVDRVIEGAYEVVGVFDRIEEKRDAMQSLILPPPARQALAQ
                     AALTYRYGNEHQPVTTADILTPRRREDYGKDLWSAYQTIQENMLKGGISGRSAKGKRI
                     HTRAIHSIDTDIKLNRALWVMAETLLESMR"
BASE COUNT          162 a          232 c          209 g          173 t
ORIGIN      
        1 cgcgtaaccg ttccggtcac agacggatgg ccagatgcca ttgcctgtac tcagtctgca
       61 cagactgagt tctccgggac ggtaaagcag acgggtacac caggtgcaca ggtcatcatc
      121 agaaatggaa cgacaccccg gcagcgcctc cggggcgtcg ggtgattctg gtacctgaat
      181 cacgccttca gggatatgtt ttatcagcgc atgctctcca gcagcgtttc tgccattacc
      241 cacaatgcgc ggttgagctt aatatcggtg tcgatgctgt gaatggcacg ggtgtggata
      301 cgttttcctt ttgcactgcg gccggaaatc ccgcctttca gcatattctc ctgtatggtc
      361 tgataagcac tccacaggtc cttaccgtaa tcctcccggc gtcgcggtgt cagaatatca
      421 gcggtggtga cgggctgatg ctcattacca taacggtaag tcagtgccgc ctgtgccagc
      481 gcctggcgtg ccggtggcgg cagaatcagc gactgcatgg catcacgctt ctcctctatc
      541 cggtcaaaaa cgcccaccac ctcgtaagcc ccttcaatca ccctgtccac cacgtttccc
      601 cggtgtggca cccggacttc tcccagcgac tgaccgcaga ccaggccatt ggtgcagatg
      661 gcacgaaaat atccgggtaa catctggtag ctggatgaac cgtcatggga gttgagcaga
      721 ataatttcag gcacatgctg accggttatc tgtccggccc gccgcagacg cagcat
//
LOCUS       DAZFLD010000641          774 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.644, whole genome shotgun sequence.
ACCESSION   DAZFLD010000641 DAZFLD010000000
VERSION     DAZFLD010000641.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..774
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.644"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            157..717
                     /gene="pagP"
                     /locus_tag="ACHKBL_005349"
     CDS             157..717
                     /gene="pagP"
                     /locus_tag="ACHKBL_005349"
                     /EC_number="2.3.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) palmitoyltransferase PagP"
                     /protein_id="HFO5822045.1"
                     /translation="MNVSKYVAIFSFVFIQLISVGKVFANADEWMTTFRENIAQTWQQ
                     PEHYDLYIPAITWHARFAYDKEKTDRYNERPWGGGFGQSRWDEKGNWHGLYAMAFKDS
                     WNKWEPIAGYGWESTWRPLADENFHLGLGFTAGVTARDNWNYIPLPVLLPLASVGYDP
                     VTFQMTYIPGTYNNGNVYFAWMRFQF"
BASE COUNT          222 a          140 c          178 g          234 t
ORIGIN      
        1 atctttaatc gatgcgcgga aatatttaac ttaaacaagc ggaaataaat agagcagcta
       61 ttcagattat tctttatgtt ggggctatta aggttatgtt aattgtagct ttgctatgct
      121 agtagtagat ttttgataaa tgttttatgg tcacaaatga acgtgagtaa atatgtcgct
      181 atcttttcct ttgtttttat tcagttaatc agcgttggta aagtttttgc taacgcagat
      241 gagtggatga caacgtttag agaaaatatt gcacaaacct ggcaacagcc tgaacattat
      301 gatttatata ttcctgccat cacctggcat gcacgtttcg cttacgacaa agaaaaaacc
      361 gatcgctata acgagcgacc gtggggtggc ggttttggtc agtcgcgttg ggatgaaaaa
      421 ggaaactggc atggcctgta tgccatggca tttaaggact cgtggaacaa atgggaaccg
      481 attgccggat acggatggga aagtacctgg cgaccgctgg cggatgaaaa ttttcattta
      541 ggactgggat tcaccgctgg cgtaacggca cgcgataact ggaattacat ccctctcccg
      601 gttctactgc cattggcctc tgtgggttat gatccagtga cttttcagat gacctacatt
      661 ccgggtacct ataacaatgg caatgtgtac tttgcctgga tgcgctttca gttttgagac
      721 aaatgaaatt ttagtaaact ctttaaaatc aatgggtaaa ataattaaca tcaa
//
LOCUS       DAZFLD010000642          774 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.645, whole genome shotgun sequence.
ACCESSION   DAZFLD010000642 DAZFLD010000000
VERSION     DAZFLD010000642.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..774
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.645"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>774
                     /locus_tag="ACHKBL_005350"
     CDS             <1..>774
                     /locus_tag="ACHKBL_005350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000382418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFO5822046.1"
                     /translation="NILTASLIIKNEGGYAINWQDFDLKIKSAKMVLLCNPHNPTGTV
                     WSEEELLKIATSCTRHNVWLCSDEIHSDFVFNRSFTSALKIKKEKVVVFNSISKTFNV
                     PALTGSYLISTDEDYNHKFRTISRYRDFVNSPSVLNIIATIVAYNECEGWLKSLKAHI
                     ASNIQFTQQYLNENTPELIVRPADGCYFSWIDCSAIGYSFDEFYSRLIHEGKVGIMAG
                     NVYGTEGEGYLRLNLACGREKLYMGLTRLASVIKNINQG"
BASE COUNT          245 a          130 c          170 g          229 t
ORIGIN      
        1 gaaatattct gacagcctct ttgattataa aaaatgaggg gggctacgct atcaactggc
       61 aagattttga tcttaagatt aaaagcgcaa agatggtgtt attatgtaat cctcataatc
      121 cgacaggaac agtctggtcg gaagaagaac tgcttaaaat cgctacaagc tgtacaaggc
      181 ataatgtctg gttatgttca gatgaaattc atagtgattt tgtttttaat cgtagcttta
      241 catccgcgct taaaataaaa aaagaaaaag tagtggtatt taactcgata tcgaaaacat
      301 ttaatgttcc tgcgctaaca ggatcgtatc tgatttcaac tgacgaggat tataatcata
      361 agttcagaac gatatcgagg tatcgcgatt tcgtaaactc cccatcagta ctgaatataa
      421 ttgcaaccat tgttgcctat aatgaatgtg agggatggtt aaagtcatta aaggcacata
      481 tcgcctctaa cattcaattc acccagcagt atctgaacga aaatactcca gaattgatcg
      541 taaggccagc tgatggttgc tatttttcgt ggatagattg ctctgctatt ggttattcct
      601 ttgatgaatt ctacagtcga ttaattcatg aaggcaaagt gggcattatg gcggggaatg
      661 tctatggaac ggagggggag ggctaccttc gtctgaacct ggcctgcggt cgagaaaaat
      721 tatatatggg gcttacgcgt cttgcgagtg taattaaaaa tattaatcaa ggaa
//
LOCUS       DAZFLD010000643          769 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.646, whole genome shotgun sequence.
ACCESSION   DAZFLD010000643 DAZFLD010000000
VERSION     DAZFLD010000643.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..769
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.646"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(108..728)
                     /gene="sodA"
                     /locus_tag="ACHKBL_005351"
     CDS             complement(108..728)
                     /gene="sodA"
                     /locus_tag="ACHKBL_005351"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010345169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HFO5822047.1"
                     /translation="MSYTLPSLPYAYDALEPHFDKQTMEIHHTKHHQTYVNNANAALE
                     SLPEFANLPVEELITKLDQLPADKKTVLRNNAGGHANHSLFWKGLKKGTTLQGDLKAA
                     IERDFGSVDNFKAEFEKAAASRFGSGWAWLVLKGDKLAVVSTANQDSPLMGEAISGAS
                     GFPILGLDVWEHAYYLKFQNRRPDYIKEFWNVVNWDEAAARFAAKK"
BASE COUNT          160 a          192 c          210 g          207 t
ORIGIN      
        1 tgtttttatt ttgaaattga tcacaaaaaa accaccgccg ttggcgatgg ttcatttttt
       61 aagctgatat gcggcgtaaa cgcctcattg cagcaggcgg caaatgatta ttttttcgcc
      121 gcgaaacgtg ccgctgcttc gtcccagttc accacgttcc agaactcttt gatgtagtca
      181 ggacggcggt tctggaattt caggtagtaa gcatgttccc acacatccag gcccagaatc
      241 gggaagccgg aagcgccaga aatagcttca cccatcagcg gggaatcctg gttagcagta
      301 gaaaccaccg ccagtttatc gcctttcagc accagccatg cccagccgga accaaagcgg
      361 gaagctgccg ctttctcaaa ttctgctttg aagttatcaa cggagccgaa gtcacgttcg
      421 atagccgctt tcaggtcacc ctgcagggtg gtgccttttt tcagaccttt ccagaacagg
      481 ctgtggttag cgtgaccgcc agcgttgttg cgcagtacgg ttttcttgtc tgctggcagc
      541 tggtccagtt tggtgatcag ctcttcaacc ggcaggttgg caaattctgg caggctttcc
      601 agcgccgcgt tggcgttgtt gacgtaggtc tgatggtgtt tggtgtggtg gatttccatg
      661 gtctgcttat cgaagtgcgg ttccagggca tcgtaagcat acggcaggga tggcagggta
      721 taactcatat tcatctccag tgttgtcggg cggccgattg ttaatgccg
//
LOCUS       DAZFLD010000644          768 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.647, whole genome shotgun sequence.
ACCESSION   DAZFLD010000644 DAZFLD010000000
VERSION     DAZFLD010000644.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 768)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 768)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..768
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.647"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>768
                     /locus_tag="ACHKBL_005352"
     CDS             <1..>768
                     /locus_tag="ACHKBL_005352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004115529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="conjugative transfer ATPase"
                     /protein_id="HFO5822048.1"
                     /translation="KKYLGNRHKLYRAGITFLLRAEDMESLKRRRVELTTVLLGAGLQ
                     PVRPEFDVGPLNSWLRALPMCFDPDTDKKQWYTRLMWVQHLAGLLPVTGRETGTGHPG
                     FSFFNRGGDVLTFDPLNKLDRTQNAHLLLFGPTGAGKSATLCGSLSQIMAVHRPRLFI
                     AEAGNSFGLLADYFESLGLSVNKISVKPGTGVCLPPFADAHQLVEQGETLQSVDEHSL
                     PDLDEDEGDEEEEKRDILGEMEISARMMITGGDPKEEA"
BASE COUNT          168 a          175 c          255 g          170 t
ORIGIN      
        1 gaagaaatat ctgggaaacc ggcacaagct ctaccgtgcc gggatcacgt ttctgctgcg
       61 ggcagaggat atggagagtc tgaagcgcag acgggttgaa ctgacgacgg tgctgctggg
      121 cgcgggctta cagccagtgc gcccggagtt tgatgtcgga ccgctgaaca gctggctgcg
      181 cgcgctgccc atgtgttttg atcctgatac ggataaaaaa cagtggtaca cgcgtctgat
      241 gtgggtacag catctggcag ggctcctgcc ggtgacggga agggaaaccg gtacggggca
      301 tcccggattc agtttcttta accgtggcgg ggatgtgctg acctttgatc cgctgaataa
      361 gctggaccgg acccagaatg cacatctgct gcttttcggg cctaccggag ccggtaaatc
      421 ggccacgctg tgtggttcgt tgtcgcagat aatggcagtt catcgtcccc gtctgtttat
      481 cgctgaggcc gggaactcgt ttggtctgct ggcggattat tttgagagtc tcgggctttc
      541 ggttaacaaa atcagtgtca aaccgggaac gggggtgtgt cttcctccat ttgcggatgc
      601 tcatcaactg gtggagcagg gagagacgct gcagagtgtg gatgagcatt cactgcccga
      661 tcttgatgaa gatgaggggg atgaagagga agagaaacgc gacattctgg gggagatgga
      721 aatatctgcc cgcatgatga tcaccggagg ggatccgaaa gaagaagc
//
LOCUS       DAZFLD010000645          767 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.648, whole genome shotgun sequence.
ACCESSION   DAZFLD010000645 DAZFLD010000000
VERSION     DAZFLD010000645.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..767
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.648"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>767
                     /locus_tag="ACHKBL_005353"
     CDS             <1..>767
                     /locus_tag="ACHKBL_005353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 104 protein"
                     /protein_id="HFO5822049.1"
                     /translation="DDSRTSHALVKHNYQRLLDKIDSGSDRYSPMEYWRALHNPDYRD
                     VLQKTIVKHPSDWYFKKDDAIWQPFLNALKKDAPEWKKYSEDFLDKMAWMQDVTTEKL
                     GPSLWHMHPIMFLASLIQPNEDIRILRLRAFLRMIRIGEGTIQEDGYRTMFTGVKFTD
                     FSKHPNIRHEANGVVSTAAGAYQFLYGTWRNLQRRYSFPDFSPSNQDLGCIALIAGRK
                     ALDVVMQGKISEAIHLCRIEWASLPGSPHAQPTANKK"
BASE COUNT          238 a          151 c          170 g          208 t
ORIGIN      
        1 cgatgattcg cgaaccagtc atgcgctggt gaagcataat tatcagcgcc tgctggataa
       61 aattgatagt ggaagcgatc gttactctcc aatggaatac tggagagcac ttcataatcc
      121 cgactatcgt gacgttcttc agaaaacaat cgttaaacat cccagcgact ggtacttcaa
      181 aaaagacgat gcaatctggc aaccatttct taatgcgctg aaaaaagatg ctccggaatg
      241 gaagaaatac agcgaagatt ttcttgataa gatggcctgg atgcaggatg tgaccacgga
      301 gaaactgggc ccttcgttgt ggcatatgca tccaataatg tttttagcct ccctaattca
      361 acctaatgaa gatattagaa ttcttcgttt acgtgcattt ctacgaatga tccgcatcgg
      421 tgaagggaca attcaggaag acggatacag aacgatgttt actggtgtaa aatttacaga
      481 ttttagtaaa caccccaata tcaggcatga agctaatggt gttgtttcga ctgctgctgg
      541 agcttatcaa tttttatacg gaacatggag aaatttgcaa cgtagatatt catttccaga
      601 tttcagtccg agcaatcaag atctcggttg tatagcgctc attgcaggtc gaaaagcatt
      661 agatgtagta atgcagggta agattagtga agccatacat ttatgtcgta ttgagtgggc
      721 cagtttaccc ggttcaccgc atgctcaacc aactgcaaat aaaaaaa
//
LOCUS       DAZFLD010000646          767 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.649, whole genome shotgun sequence.
ACCESSION   DAZFLD010000646 DAZFLD010000000
VERSION     DAZFLD010000646.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..767
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.649"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>767
                     /locus_tag="ACHKBL_005354"
     CDS             <1..>767
                     /locus_tag="ACHKBL_005354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004171002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system Vgr family protein"
                     /protein_id="HFO5822050.1"
                     /translation="EGIVITTVRSRGSRKSRFTLTFEGIPYSETVCWRPALLSRPVIS
                     GSLPARIESHEKNDIYAHLDDQGRYRVKLDFDRNSTERGYAYLWLRLAKPCAGDTYGF
                     HSPLIDGTEVAIVFDGGDPDRPYIAYALHDSEHPDHVTSDNHTRNVWRTPANNKLRME
                     DKRQEEHIKLATEYGKTQLNMGHLVNSRREKRGAGFELRTDEHGAVRAAKGLFLTADA
                     QARAQGPVLEMAAAISQINQANSQMQALNSAAEAAGAL"
BASE COUNT          179 a          207 c          236 g          145 t
ORIGIN      
        1 gaaggcatcg ttatcaccac ggtgcgttcc cgcggttcac gcaaaagtcg ctttacgctg
       61 acgtttgagg ggatccccta cagtgaaacg gtctgctggc gtccggcact gctcagtcgc
      121 ccggtgatat ccggctcgct tccggcgcgg attgagagtc acgagaagaa cgatatctac
      181 gctcatctgg acgatcaggg gcgttaccgg gtgaagctgg actttgaccg caacagtaca
      241 gaacggggtt acgcttatct gtggttgcgt ctggctaaac cctgtgccgg tgatacgtac
      301 ggtttccact caccgcttat cgacggaacg gaagtggcca ttgtatttga cggcggagat
      361 ccggatcgtc cctacatcgc ttatgccctg catgattcag agcatccgga tcatgtcacc
      421 agtgacaatc atacgcggaa cgtgtggcgc acgccggcga acaataagct gcggatggag
      481 gataagcgcc aggaagagca tatcaagctc gcaacggaat atggcaaaac acagctgaac
      541 atgggacacc tggtcaacag ccggcgtgaa aagcgaggtg ccggttttga actgcgaacg
      601 gatgagcatg gggctgtacg tgcagcaaaa gggctgttcc tgacggcaga tgcccaggcc
      661 agagcccagg ggccggtgct ggaaatggcg gccgcgataa gtcagattaa ccaggctaac
      721 agccagatgc aggccctgaa cagcgctgcc gaagccgccg gcgccct
//
LOCUS       DAZFLD010000647          759 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.650, whole genome shotgun sequence.
ACCESSION   DAZFLD010000647 DAZFLD010000000
VERSION     DAZFLD010000647.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..759
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.650"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..386
                     /locus_tag="ACHKBL_005355"
     CDS             <1..386
                     /locus_tag="ACHKBL_005355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5822051.1"
                     /translation="IFMIKTLFDPETMSREDMLREIQLLRDSRDLALELVFGYPETKA
                     AEQIELNDDEITLLKVKLPEHHFQWLTGDGDEALTVGRLGDFADLADDFSVLAGEIDP
                     DLEEPQEEVISRGPCGVVFCLIEPD"
BASE COUNT          236 a          142 c          198 g          183 t
ORIGIN      
        1 atatatttat gattaaaacc ttgttcgatc cagaaaccat gagcagggag gatatgctta
       61 gggaaattca gctactgcgc gactcacgtg atctggcgct tgaactggtg ttcggctatc
      121 cagaaacaaa ggccgcagaa caaattgaat tgaatgatga tgaaataacc cttctgaaag
      181 tgaaactacc ggaacaccat tttcaatggt taacaggaga tggagatgag gcgctaacag
      241 ttgggcgatt aggtgatttt gcagatctgg ctgacgattt tagcgttctg gccggagaga
      301 tagatccaga cctggaagaa ccacaggaag aagttatatc ccgtgggcct tgtggtgtcg
      361 tattttgttt gattgagcct gactgatctt gtgtgtgagg tgcagtaaat gccgcgtctg
      421 cggcatggga ggcgacaaaa acaagttaaa tataattggc tgtaaagtgt cgccgtgacg
      481 cggctacgcc gcccggatgt aaataaatca caggaacacc tcctccccct gccagacgtg
      541 tttgtgtctg gcgcagaatg cgccagacaa ccatatatca ggtaacacgt tccttaatgg
      601 tcgaagacaa gcaattgtaa cagtaacagg cttgaacttt atggagatga tgaaatggaa
      661 gattggcgta ctaagccaag aacaggagcg tctcaatttg ctgggtatga agaggaaata
      721 agattattaa aggaaaagaa atatacgaac aaacaaata
//
LOCUS       DAZFLD010000648          756 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.651, whole genome shotgun sequence.
ACCESSION   DAZFLD010000648 DAZFLD010000000
VERSION     DAZFLD010000648.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..756
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.651"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(535..>756)
                     /locus_tag="ACHKBL_005356"
     CDS             complement(535..>756)
                     /locus_tag="ACHKBL_005356"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5822052.1"
                     /translation="YISPAKVIRHAGYIIRESGSERRYLLPQSYLINPYQQGNSMAKS
                     SNYAPVKVIIYVDIGKDPCHTVIINRSDN"
BASE COUNT          210 a          159 c          184 g          203 t
ORIGIN      
        1 ctcaccttca cggtaatatg catctggatt tctgcaacag gtcagcgata agatgctctg
       61 cctcctcctt cagagcagaa gtaaatacag ccatgatgga taaaacttaa ccagaaaaaa
      121 taagaggagg caagtaagtt gcctggcatg tttatgacaa taaaaagttc cgtcaggcat
      181 catggcgaac aggatatcac agcgccgttt taccggggca atcagtgaat ggtggtgtgg
      241 ttcattggta agccccggat atcaggtcac tgagtatatt catgaatgac cgttaaaccg
      301 gggatgcgag ctgctccccg ggaaagtcga actgactggt caatagatat caatacgggg
      361 cagtcagtcc acttacaggt atctgacaag gacaccttct gagcgtgcaa tgtggacggg
      421 taatacaacg atggtagcag gctggtcagc aatcagcagt atatgcccat atttcctgag
      481 gccggatatc tgtttcctga gcttgttttc gacgtttgct aatattttgt cgaactaatt
      541 atcagaacga ttaatgataa cagtgtggca gggatcttta ccgatatcaa cgtaaataat
      601 gaccttaaca ggtgcgtaat tgctggattt tgccatgctg ttaccctgtt gatacgggtt
      661 aattaaataa ctttggggaa gcaggtaccg gcgttcacta ccgctctccc tgataatgta
      721 tcctgcatgc ctgataactt ttgccgggct aatgta
//
LOCUS       DAZFLD010000649          753 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.652, whole genome shotgun sequence.
ACCESSION   DAZFLD010000649 DAZFLD010000000
VERSION     DAZFLD010000649.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..753
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.652"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            173..248
                     /locus_tag="ACHKBL_005357"
     tRNA            173..248
                     /locus_tag="ACHKBL_005357"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:206..208,aa:Ile,seq:cat)
BASE COUNT          211 a          148 c          175 g          219 t
ORIGIN      
        1 taacatgctt gttgactgcg agtatcgact ttgcgaatgg aagcggtgtc gggatttatg
       61 tcgcatgtat tgacgaccca ggatggatga gaacgcataa aaatctgtta tttacatcat
      121 aagatcatca tgtttattgc gtggtttata agtattgtta ttgcatactt aaggcccttt
      181 agttcagtgg ttagagcagg cgactcataa tcgcccggtc gctggttcaa gtccagcagg
      241 ggccaccaaa tcatcattca tgtatcaaga agagttaagg gtacaagggg tactcgatgg
      301 cggtctatat ttcatgacta acgccattta aaggaagaag tgcggtgggt ggtttcgaat
      361 gagggctggt gttttggtac gtggcacgcc actgcaatgt atttatccat cagtactcat
      421 tcaaaagtac ctggaaaaaa tgcccgctat gccaagcggg caaattgctt acatgtggaa
      481 ttaagtaatt aacgataaag ccagagtatt atcatttatc atgcctcttc atttaatcag
      541 gttaattact caaatcactg aactattctc tgttcgctca ctgatgataa gtgaagtgtc
      601 acaaccacga tttgtatgtg gaccaatgcc gtagagtaag gatgaaatgt ctctgccacg
      661 ccttaaatat ctactactga ccagaacacc cgccgcaggc agatgagtct tatgagcgtt
      721 acaggagtct ggctttgctc attaattaac gct
//
LOCUS       DAZFLD010000650          752 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.653, whole genome shotgun sequence.
ACCESSION   DAZFLD010000650 DAZFLD010000000
VERSION     DAZFLD010000650.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 752)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 752)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..752
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.653"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>752
                     /locus_tag="ACHKBL_005358"
     CDS             <1..>752
                     /locus_tag="ACHKBL_005358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001070133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5822053.1"
                     /translation="PTEAVSAGNIADPFRPRYAVNLQLLDENGNPAADTPVYNAVPLP
                     VPMAGSESGMFQFPPPGTLVEVGFVEGRADKPFIRQIMAEGHNLPDVKPGEQLQQQRD
                     GVSQRVTVAGDWERQTDQRIQERSRERVVSADEEKRSLTSRETTIQATDTTTVLGVST
                     LQAGAVTHITEENYSISTGGHMMVVAGTCERDVEGDTQDTVGGSLTEKIKGVRHSIAQ
                     AQKLVGDTVKLGNEGINVLTMLTDLADVVEELA"
BASE COUNT          170 a          223 c          249 g          110 t
ORIGIN      
        1 ccgaccgaag ccgtcagcgc gggcaatatt gccgacccgt tcagaccccg ctacgcggtg
       61 aatctccagc tgcttgacga gaacggcaac ccggcagcgg atacgcccgt ctataacgcc
      121 gttcccctcc ccgttcccat ggcgggcagt gagtccggca tgttccagtt cccgccgccc
      181 ggaaccctgg tggaagtggg atttgtggaa ggccgggcgg ataagccctt catccgccag
      241 ataatggcag agggccacaa tctgcccgac gtgaagccgg gcgagcagtt gcagcaacag
      301 cgcgacggcg tatcgcagcg cgtgacggtc gccggagact gggagcgcca gacagaccag
      361 cggatacagg agagatcacg cgagcgcgtg gtgagcgcag acgaggaaaa gcggagcctg
      421 accagccggg agaccaccat acaggcaacc gacacaacca ccgtcctggg cgtatccacg
      481 cttcaggctg gcgcggtcac ccatatcact gaagagaact acagcatcag cacgggcggg
      541 cacatgatgg tggttgccgg tacctgtgag cgcgacgtgg aaggcgacac ccaggacacc
      601 gtgggcggca gccttaccga aaagattaag ggcgtccgtc acagtattgc gcaggcgcag
      661 aagctggttg gcgatacggt gaagctggga aacgagggca ttaatgtact gaccatgctg
      721 acggaccttg ccgatgtggt ggaagagctg gc
//
LOCUS       DAZFLD010000651          746 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.654, whole genome shotgun sequence.
ACCESSION   DAZFLD010000651 DAZFLD010000000
VERSION     DAZFLD010000651.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..746
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.654"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..490
                     /locus_tag="ACHKBL_005359"
     CDS             <1..490
                     /locus_tag="ACHKBL_005359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000367546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HFO5822054.1"
                     /translation="TKDRIIKAGKKLKKDNPNYNGDLGFKIFETVHDFRAKDESELTL
                     SNLSFFDDAVLTPEQYDTLLTTWCVYDGSLLTTPIEDIDLNGYKAHLCDGRLYLIAPN
                     FTSEALKALLQKLDADKDFAPNKVVFYGSNFESAKQMELNEALKSYANKKSIDLDLVV
                     RN"
     gene            493..>746
                     /locus_tag="ACHKBL_005360"
     CDS             493..>746
                     /locus_tag="ACHKBL_005360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015974229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5822055.1"
                     /translation="MSKGFTFEKNLPHQKAGVDSVMNVFVSAIPHQTDHVAIRLLANP
                     ELNLSEQQYYNNIKNVQEFNGIEHSKDNYDAKSNVIDVSM"
BASE COUNT          262 a          127 c          145 g          212 t
ORIGIN      
        1 aacaaaagac agaattatta aggcaggaaa aaagctgaaa aaagataatc ctaattataa
       61 tggagatttg ggatttaaaa tatttgaaac agttcatgat tttcgagcga aagatgagtc
      121 tgaacttacg ctttctaatc ttagtttctt tgacgatgct gtattgacac cagagcagta
      181 tgacactcta ttgacgacat ggtgcgtgta tgatggcagc ttgctaacaa caccaattga
      241 agatattgac cttaatggct acaaagcaca cttgtgcgat ggtcggctat atctaattgc
      301 gcctaatttc acaagtgagg ctttgaaagc tttacttcaa aagctggatg cagacaaaga
      361 ctttgcccct aacaaagttg tattttatgg cagcaacttc gaaagtgcga aacagatgga
      421 actcaatgaa gcactaaaaa gttatgctaa taaaaaatct attgatttag atttggtggt
      481 aaggaactaa tcatgtctaa agggttcaca ttcgaaaaaa atttacctca tcaaaaggct
      541 ggcgttgatt cggtaatgaa tgtctttgta tccgccatac ctcatcagac ggatcatgtt
      601 gcaattcgcc tgttagctaa tccagaactg aatctatctg aacaacaata ctacaacaac
      661 ataaaaaatg ttcaggagtt caatggtatt gagcactcaa aagataatta cgatgctaaa
      721 agcaacgtaa ttgatgtttc tatgga
//
LOCUS       DAZFLD010000652          746 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.655, whole genome shotgun sequence.
ACCESSION   DAZFLD010000652 DAZFLD010000000
VERSION     DAZFLD010000652.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..746
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.655"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>746
                     /locus_tag="ACHKBL_005361"
     CDS             <1..>746
                     /locus_tag="ACHKBL_005361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GPO family capsid scaffolding protein"
                     /protein_id="HFO5822056.1"
                     /translation="RNTYTAMLWPCHENDTSYRQYTFNLGEVDALKVETREDGKTRLY
                     ARLVPNQYLIEANRLGQKLFTSAELIPNFAKSGRDYLMGVAVTDIPASLGTEKIRFTL
                     NNEEYTAQRANCQTFTLGSLSPDTTPAPRKPESFLKRLFSAGQTDNTDTSKPDTGEDA
                     KMDEIKALLEKLITLVEGGSAAAAGEGTADTPEEAKAEVAEIAAEIADAAAEVAELAE
                     EVEAAPEDEVKAEAFSAAKAKLEDAIKAFS"
BASE COUNT          208 a          218 c          213 g          107 t
ORIGIN      
        1 cccgcaacac ctacacggcg atgctgtggc cctgccatga gaacgacacc agttaccgcc
       61 agtacacctt caatctgggc gaagtggacg cgctgaaagt ggaaacccgc gaggacggta
      121 aaacccgtct ttacgcgcga ctggtcccta atcagtacct gattgaagcc aacaggctgg
      181 ggcaaaaact cttcacatca gccgaattaa taccgaactt tgccaaaagc ggacgcgact
      241 atctcatggg tgtggcggtg acggatatcc ccgccagtct ggggacggaa aaaatccgct
      301 tcaccctgaa caacgaggaa tacacggcac agcgggcgaa ctgccagacg ttcacgctgg
      361 gcagcctgtc accggacacc acccctgccc cccgtaaacc tgagtcattc ctgaaacgcc
      421 tgttcagcgc aggacagacc gacaacaccg ataccagtaa gcctgataca ggcgaggacg
      481 caaaaatgga cgaaattaaa gcactgcttg aaaagctgat caccctggtg gaaggaggca
      541 gcgccgctgc cgctggcgag ggcacagcag acacaccgga agaagcaaaa gccgaagtcg
      601 cggaaatcgc cgccgaaatc gccgatgccg ccgcagaggt ggcagagctg gcagaagagg
      661 tggaagccgc cccggaagat gaggttaagg ctgaagcctt cagcgccgcg aaagcgaagc
      721 tggaagacgc gattaaagcg ttcagt
//
LOCUS       DAZFLD010000653          746 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.656, whole genome shotgun sequence.
ACCESSION   DAZFLD010000653 DAZFLD010000000
VERSION     DAZFLD010000653.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..746
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.656"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
BASE COUNT          207 a          187 c          155 g          197 t
ORIGIN      
        1 gtggaatatc gggaaaacag gaagttacgt taaaagccga aggaaaaact cacaatccct
       61 tcttcaatca gcctctgaaa atcggctttt cagcatgtct tcccccattg ttcgtatacc
      121 ctgttcgatg gattattatt gatccatatg atcgcttcac gtaaacacga ttctggctcc
      181 acaccattct ggacgcgatt gttaccaaaa aagacaacca tcacaagacg tcacagacgg
      241 tgcgtgcgct tactcctgta tatctggcgc aacagaatct accagtgtaa actctctatg
      301 cttttgcgca gaagcagtgg gcctgggttc gcttatgtcc tgcatctaca agcgaatgac
      361 tgaccagaga ctcccatatc tggtcagtcg cccattttta ttttccctat acactcagac
      421 tacaatagcc gtaacaaaca gcataccccc agtcatagtg gcctctttat cttcttatgg
      481 cttgtctgtg gtactggaga tagagcaact gtgggagtac tgttatggcc cccggtaaga
      541 aaaactcttg cttaatgtac agcagaactg ttcccagaaa gaagcctgtg catattttca
      601 tagaaggaga tcacccataa cggcgttttt cacggtaata cacgccaggt aaagaatgct
      661 ggattatcaa tcaaagctcc gcacaggaag cgcttatgcc gtagagcgcc ctctggagaa
      721 aagtgctact tcttgtcgat tcagac
//
LOCUS       DAZFLD010000654          744 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.657, whole genome shotgun sequence.
ACCESSION   DAZFLD010000654 DAZFLD010000000
VERSION     DAZFLD010000654.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..744
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.657"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            139..729
                     /locus_tag="ACHKBL_005362"
     CDS             139..729
                     /locus_tag="ACHKBL_005362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO5822057.1"
                     /translation="MSKKLGFALSGIMLAMAAGTAFAANDMDGGQLNISGLVVDNTCE
                     ARVDGGNKDGLILLQTATVAEITDGVLDTTVGAKAKPFSITIDCSKANPAPGTTAKMT
                     FGSVFFSNSKGTLNNDMSINNPSDGVNIALHNIEGSTIKQVQVNNPGDVYSKTLDATS
                     KTATYDFKASYVRADASKAATAGYVKTNSAYTITYQ"
BASE COUNT          218 a          150 c          159 g          217 t
ORIGIN      
        1 gatgaatgaa tgtaatagtt ggtaaacaac agacaaacat cattaacaca gtaacgattt
       61 tgttacgcag cattgcagca gactcttatc gtcatgcggt gcggttatct ctctatggaa
      121 tattattcaa ggatttttat gtctaaaaaa ttaggttttg cgctgtctgg tattatgttg
      181 gcaatggctg ctggtactgc atttgctgct aacgatatgg acggtggtca gttaaatatt
      241 agcggcctgg tcgttgataa cacctgtgaa gctcgtgttg atggcggtaa caaagatggt
      301 ttgatcctgc tgcaaaccgc tactgttgct gaaatcaccg atggtgtact ggataccacc
      361 gtaggtgcta aagctaagcc attcagcatt actattgact gcagcaaagc taacccggct
      421 ccgggtacta ctgcaaaaat gactttcggt tccgtgttct ttagcaatag caaaggtact
      481 ctgaataacg atatgtctat caataatcca tctgatggtg tgaatattgc cctgcataat
      541 attgaaggtt ccactattaa gcaggttcag gttaataacc cgggtgatgt ttacagcaaa
      601 actctggatg caacgtctaa aactgcaacc tacgatttta aagcatccta tgttcgtgct
      661 gatgcaagca aagcagcaac tgccggttat gtaaaaacta actcagcata taccattact
      721 taccagtaaa tatcagccac ctaa
//
LOCUS       DAZFLD010000655          742 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.658, whole genome shotgun sequence.
ACCESSION   DAZFLD010000655 DAZFLD010000000
VERSION     DAZFLD010000655.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..742
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.658"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>742
                     /locus_tag="ACHKBL_005363"
     CDS             <1..>742
                     /locus_tag="ACHKBL_005363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO5822058.1"
                     /translation="WSLYTLALTKVRSVIIATSLAARLAGVSFLTMSAPVLLVAAAIA
                     ALVVVAIKFWQPVKAFIRGFIDGFTGALDAMSPVTAAFGDIARALTPVWQGIKTLFGA
                     FSDLFAPVQLTTGQMNSVTRAGETCGRVVAAAVSLISPALEVVISVIRTLVDIFAIVI
                     RGWQDVVAAFDPTAPVESFRKMAGIIGNVFGDLWNVIKNSFNRTWNWLAEKLNKIPGI
                     SIDMKPTEQVNTMTRGATGSTVPPQIAET"
BASE COUNT          149 a          219 c          220 g          154 t
ORIGIN      
        1 ctggagtctg tacacgctag cactcacaaa agtacgcagc gtcatcattg ccaccagtct
       61 tgccgcgcgt ctggcgggcg tgtcgtttct gaccatgagc gccccggtcc tcctggttgc
      121 cgccgccatc gccgctctgg tggtggtggc cattaagttc tggcagcccg tcaaagcctt
      181 tattcgcggc tttattgatg gttttaccgg cgcactggac gccatgagcc cggtgactgc
      241 cgcgttcggc gacattgccc gtgcgctgac cccggtatgg cagggcataa aaaccctgtt
      301 cggtgccttc tccgatctgt ttgcaccggt acagctgacc acgggacaga tgaacagcgt
      361 cacccgtgcc ggggaaacat gcggtcgcgt ggtggccgca gcggtaagcc tgatatcccc
      421 cgcgctggag gtggttatca gcgtgatcag aaccctggtc gacatcttcg ccatcgtcat
      481 cagagggtgg caggacgtgg tggctgcatt tgacccgact gccccggtcg aatccttccg
      541 gaaaatggcg gggatcatcg gtaacgtctt cggggactta tggaacgtca ttaaaaactc
      601 gtttaaccgg acgtggaact ggcttgcgga aaagctgaac aaaatccccg gtatcagtat
      661 cgacatgaag cccacggagc aggtgaacac catgacgcgg ggcgcaacag gcagcaccgt
      721 acctccgcag attgcagaaa cc
//
LOCUS       DAZFLD010000656          742 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.659, whole genome shotgun sequence.
ACCESSION   DAZFLD010000656 DAZFLD010000000
VERSION     DAZFLD010000656.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..742
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.659"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            95..>742
                     /locus_tag="ACHKBL_005364"
     CDS             95..>742
                     /locus_tag="ACHKBL_005364"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO5822059.1"
                     /translation="MGAPVLLVVAAIAALVVVAIKFWQPVKAFIRGFIDGFTGALDAM
                     SPVTAAFGDIARALAPVWQGIKTLFGAFSDLFAPVQLTTGQMNSVTRAGETCGRVVAA
                     AVSLISPALGVVISVIRNLIDIFTIVIRGWQDVVAAFDPTAPVESFRKMAGIIGNVFG
                     DLWNVIKNSFNRTWNWLAEKLNKIPGISIDMKPTEQVNTMTRGATGSTVPPQIAET"
BASE COUNT          147 a          218 c          223 g          154 t
ORIGIN      
        1 ctggagtctg tacacgctcg cactcacaaa agtacgcagc gtcatcattg ccaccagtct
       61 tgccgcgcgt ctggcgggcg tgtcgtttct gaccatgggc gccccggtcc tcctggttgt
      121 tgccgccatc gccgctctgg tggtggtggc cattaagttc tggcagcccg tcaaagcctt
      181 tattcgcggc tttattgatg gttttaccgg cgcactggac gccatgagcc cggtgactgc
      241 cgcgttcggc gacattgccc gggcgctggc cccggtatgg cagggcataa aaaccctgtt
      301 cggtgccttc tccgatctgt ttgcaccggt acagctgacc acgggacaga tgaacagcgt
      361 cacccgtgcc ggggaaacat gcggtcgcgt ggtggccgca gcggtaagcc tgatatcccc
      421 cgcgctgggg gtggttatca gcgtgatcag aaacctgatc gacatcttca ccatcgtcat
      481 cagagggtgg caggacgtgg tggctgcatt tgacccgacc gccccggtcg aatccttccg
      541 gaaaatggcg gggatcatcg gtaacgtctt cggggactta tggaacgtca ttaaaaactc
      601 gtttaaccgg acgtggaact ggcttgcgga aaagctgaac aaaatccccg gtatcagtat
      661 cgacatgaag cccacggagc aggtgaacac catgacgcgg ggcgcaacgg gcagcaccgt
      721 acctccgcag attgcagaaa cc
//
LOCUS       DAZFLD010000657          739 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.660, whole genome shotgun sequence.
ACCESSION   DAZFLD010000657 DAZFLD010000000
VERSION     DAZFLD010000657.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..739
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.660"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..652)
                     /gene="queE"
                     /locus_tag="ACHKBL_005365"
     CDS             complement(<1..652)
                     /gene="queE"
                     /locus_tag="ACHKBL_005365"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HFO5822060.1"
                     /translation="MQYPINEMFQTLQGEGYFTGVPAIFIRLQGCPVGCAWCDTKHTW
                     EKLEDREVSLFSILAKTKESDKWGAASSEDLLAVIGRQGYTARHVVITGGEPCIHDLL
                     PLTDLLEKNGFSCQIETSGTHEVRCTPNTWVTVSPKLNMRGGYEVLSQALERANEIKH
                     PVGRVRDIEALDELLATLTDDKPRVIALQPISQKDDATRLCIETCIARNWRLSMQTH"
BASE COUNT          174 a          202 c          170 g          193 t
ORIGIN      
        1 tatgtgtttg catcgacaaa cgccaattac gcgcaatgca ggtttcaatg cacaaacgtg
       61 tggcatcatc cttttggcta atcggctgca gtgcaatgac tcgcggttta tcatcggtca
      121 gcgttgccag tagttcatcc agtgcttcaa tatcacgtac gcgccccact ggatgcttga
      181 tttcgttggc tcgctccagt gcctgtgaca acacttcata gccgccgcgc atgttcagct
      241 ttggtgatac ggtaacccag gtattcggtg tgcagcgtac ctcatgagta ccactggttt
      301 caatctggca gctaaaacca ttcttttcga gcagatcagt cagtggtagc aaatcatgaa
      361 tgcaaggctc accacccgta atcaccacat gccgcgcggt gtatccctgg cgaccaatga
      421 cagccagcaa gtcttcactg ctcgccgccc cccacttatc actctctttg gtcttcgcca
      481 gaatgctgaa aagggagact tcccgatcct caagcttttc ccaggtgtgt ttggtgtcgc
      541 accaggcaca gccaaccggg catccctgta aacgaataaa aatggcgggg acgccggtaa
      601 agtaaccctc accttgcagg gtctggaaca tctcgttaat cgggtactgc atagcattct
      661 ctgtgaagtg gataattgtt aattattgca gatcctgcca caacaatcat gtcttattaa
      721 cattctgtta caggcaggt
//
LOCUS       DAZFLD010000658          736 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.661, whole genome shotgun sequence.
ACCESSION   DAZFLD010000658 DAZFLD010000000
VERSION     DAZFLD010000658.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..736
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.661"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..282
                     /locus_tag="ACHKBL_005366"
     CDS             <1..282
                     /locus_tag="ACHKBL_005366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5822061.1"
                     /translation="RDGLKALGAERHHYINLARTENRFAGLAVGERQSAGEQQLGLLI
                     VMQKIEQAAFDEAIQSGMTPTEAVREVARRMETFASLMTSAPSLGVNHA"
     gene            275..523
                     /locus_tag="ACHKBL_005367"
                     /pseudo
     CDS             275..523
                     /locus_tag="ACHKBL_005367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001526648.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="antA/AntB antirepressor family protein"
BASE COUNT          182 a          174 c          214 g          166 t
ORIGIN      
        1 cgtgacgggc ttaaagcact gggcgcagaa cgtcaccatt acatcaacct tgcccgcact
       61 gaaaatcgct tcgctgggct ggctgtaggc gagcgtcaga gcgcgggaga gcaacagtta
      121 ggcttactga tagtcatgca gaaaatcgag caggcagcat ttgacgaggc gatacagagc
      181 ggcatgacac cgaccgaagc ggtgcgggaa gttgctcgcc gcatggagac gttcgccagc
      241 ctgatgacgt cagcgccgtc tctgggggtg aatcatgcgt aattatgatt tcgccgcaat
      301 tattccggta tcacctggca ttattggcag atatgaaacg ggcgttgtca gcgcacgcgc
      361 actgcataaa gcgctcggtg taggccgtga tttcaccacc tggatcaaag ggcgtattga
      421 ggagtacgga tttaaaaaag gtgtggattt tgaggttgta gaaaatttgt actttgattc
      481 cccgaaacgg gggagccaaa gtgtaaatat tgaacagttt gattctggct ggcacgtcgc
      541 ctgggagaac gtgaacatgc ctgacatccg caacgctgcc gccctgtaca tcgatactgc
      601 ggataagttc gcacaggtga gcatcaacca tgctgattac atgcgccata tcgtgagcca
      661 tttcgccgct gagcgcatga aacacgttaa gcaatacttg ggggaggttt tatcaaatta
      721 tcgcgctggt ggccct
//
LOCUS       DAZFLD010000659          736 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.662, whole genome shotgun sequence.
ACCESSION   DAZFLD010000659 DAZFLD010000000
VERSION     DAZFLD010000659.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..736
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.662"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>736
                     /locus_tag="ACHKBL_005368"
     CDS             <1..>736
                     /locus_tag="ACHKBL_005368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001001722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFO5822062.1"
                     /translation="AKQFNTSIIVYLIDPKYFADLPTSQFWSYATYFRVLSFEYLSES
                     ISTLLYLDADVVCKGSLKPLTEIIFKDEFAAVIPDNDSTQAACAKRLNIPEMNGRYFN
                     AGVIYVNLKKWHEANLTPYLLTLLRGETKYGSLKYLDQDALNIAFNMNNIYLAKDFDT
                     IYTLKNELHDRSHRKYQQTITDKTVLIHYTGITKPWHSWAGYPSASYFNIAREQSPWK
                     KYPLKEARTVAEMQKQYKHLFAHGEYI"
BASE COUNT          234 a          142 c          139 g          221 t
ORIGIN      
        1 ggcaaaacaa tttaatacct caattattgt atatttaatt gaccccaaat actttgctga
       61 tctgccgacg tcacagtttt ggtcgtacgc aacatacttc agggtattgt cttttgaata
      121 tctgagtgaa agtatttcta cactgctgta tctggatgcc gatgttgttt gtaaaggaag
      181 cctgaaacct ctcacagaaa ttatatttaa agatgagttt gctgcggtca ttcctgacaa
      241 tgatagtact caggcggcat gtgcaaaacg cctcaacatt cccgaaatga atggacgtta
      301 tttcaatgca ggcgttatct atgtcaatct taaaaaatgg catgaagcaa atttgacacc
      361 gtatttactc acgcttttac gaggggaaac taaatatggc tctcttaaat atttagatca
      421 ggatgcgttg aatatcgcat ttaatatgaa taatatctac ctcgcgaagg attttgatac
      481 tatttatacc ctgaaaaacg aacttcatga tcgtagtcat cgaaagtatc agcaaaccat
      541 taccgataaa acagtattga ttcactatac agggataact aaaccatggc atagctgggc
      601 tggatatcca tctgcatcat actttaatat cgcgcgtgaa caatctccct ggaagaaata
      661 tcctcttaaa gaggcgcgga ctgttgcaga aatgcagaaa caatataagc atctgtttgc
      721 ccatggtgag tatatt
//
LOCUS       DAZFLD010000660          735 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.663, whole genome shotgun sequence.
ACCESSION   DAZFLD010000660 DAZFLD010000000
VERSION     DAZFLD010000660.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..735
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.663"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..260)
                     /locus_tag="ACHKBL_005369"
     CDS             complement(<1..260)
                     /locus_tag="ACHKBL_005369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5822063.1"
                     /translation="MSELSSRPAREPVVYTLEQVSTIPEKQWHAFVLAVTETFWQLPE
                     ALRPQNAYFGSLTRASELFPVTDTLAFYCRSADGLWSVNVTI"
     gene            complement(342..719)
                     /locus_tag="ACHKBL_005370"
     CDS             complement(342..719)
                     /locus_tag="ACHKBL_005370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000287229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5822064.1"
                     /translation="MYSPLIAHYSALQTQSALLAHISQMEGELMRLRAWQRLGITPEP
                     DDETEPSLVYVYLWDTGEALGWLLYDLEQEEISAPGIQARQALDSLMALGREINHEIW
                     ADSISTGKMVAGADEYFARHDMM"
BASE COUNT          187 a          224 c          177 g          147 t
ORIGIN      
        1 tcaatagtga cattcaccga ccacagcccg tcggcagacc ggcagtaaaa cgccagcgtg
       61 tccgttaccg gaaacaactc tgacgcccgg gtcagggagc cgaaatacgc attctgcggc
      121 cgtaacgcct ccggcaactg ccagaaggtt tcagtgacag ccagaacaaa agcatgccac
      181 tgcttttccg gtattgtgga aacctgctca agggtatata ccaccggctc acgggccgga
      241 cgtgaagata actcagacat ggaacattcc tcatggttaa aacgaaacaa cggggaatgt
      301 ctcccggacg gagaactccc ccgtcagggt caggtcatga attacatcat gtcgtgccgg
      361 gcaaaatatt catccgctcc ggccaccatc tttccggtac tgatactgtc agcccagatt
      421 tcatgattaa tttcacgccc cagagccatc agactgtcga gtgcctgacg ggcctgaatt
      481 cccggtgcgg aaatctcctc ctgctccagg tcatacaaca gccagcccag cgcctcgccg
      541 gtatcccaca gatacacgta taccagtgaa ggctccgtct catcatccgg ctccggggta
      601 atacccagcc gttgccaggc acgtaaccgc atcagctcgc cttccatctg actgatatga
      661 gccagcagag cagactgcgt ctgaagcgct gaataatgag caattaaagg tgaatacatg
      721 atgacctccg tctct
//
LOCUS       DAZFLD010000661          733 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.664, whole genome shotgun sequence.
ACCESSION   DAZFLD010000661 DAZFLD010000000
VERSION     DAZFLD010000661.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..733
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.664"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..441
                     /locus_tag="ACHKBL_005371"
     CDS             <1..441
                     /locus_tag="ACHKBL_005371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Imm72 family immunity protein"
                     /protein_id="HFO5822065.1"
                     /translation="NLGAYNYFIRGMNAPRQVYYDVLIESDTEMVVPDDPIRYRDVHW
                     RLVWEDTRYSDGIIPDESEYFLDDAPGKRITCPSGELCPHSGHWATIAGGHQQFIDVQ
                     AGQLMPEATKYQSNMYADEIHLPAIWNLLRRDDEGSVYVKTGES"
     gene            519..>733
                     /locus_tag="ACHKBL_005372"
     CDS             519..>733
                     /locus_tag="ACHKBL_005372"
                     /inference="COORDINATES: protein motif:HMM:NF024894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4123 domain-containing protein"
                     /protein_id="HFO5822066.1"
                     /translation="MDGQRYFGESLSVQQPAAVPLFDTWPDSRIAFAGPWVMEMNSIT
                     DFRERLCELEAALPSVSWMISSSSLTEL"
BASE COUNT          183 a          160 c          190 g          200 t
ORIGIN      
        1 aacctcggtg cttataatta ttttattcgt ggaatgaatg ctcctcgcca ggtgtattac
       61 gacgtattaa ttgaaagtga cacagaaatg gtcgtacccg atgacccaat ccgttaccgg
      121 gacgttcact ggcgtctggt ctgggaagac acgcgctaca gtgacggaat tattcccgat
      181 gagtcagaat actttctgga tgatgcacca ggcaaacgca ttacctgccc gagtggggag
      241 ctttgcccgc attcaggtca ttgggcgacc atagccggag gacatcagca gtttattgat
      301 gttcaggccg ggcagttgat gccagaagca actaaatatc agagcaatat gtatgcagat
      361 gaaatccatc ttcctgcgat atggaacctt ttacgccggg atgatgaggg gagtgtttat
      421 gtaaaaacag gggaaagtta aataactgat caacgctttt tgacggacag atctgttacc
      481 acctgatttg ctcaacaaaa gtgacattat ttgctctggt ggatggtcag cggtatttcg
      541 gtgagagcct gtctgttcaa caacctgccg ccgtgcccct gttcgatacc tggcctgatt
      601 cgcgtattgc atttgcgggg ccatgggtta tggagatgaa cagtataacg gatttcagag
      661 aaagactgtg tgagcttgaa gctgcgcttc cgtcagtttc ctggatgata tcttcttcct
      721 cgcttactga act
//
LOCUS       DAZFLD010000662          733 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.665, whole genome shotgun sequence.
ACCESSION   DAZFLD010000662 DAZFLD010000000
VERSION     DAZFLD010000662.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..733
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.665"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..264
                     /locus_tag="ACHKBL_005373"
     CDS             <1..264
                     /locus_tag="ACHKBL_005373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000323308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlgM"
                     /protein_id="HFO5822067.1"
                     /translation="AQITAAAEDAEIHHDYARALILWQHAYHAATLTLNKNLAVAKID
                     FCIKRIRMHEQMSRIIRKTGTDEILFRLSKHHHLYEKKKTKEG"
     gene            complement(652..>733)
                     /locus_tag="ACHKBL_005374"
     CDS             complement(652..>733)
                     /locus_tag="ACHKBL_005374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_062863141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="subtilase cytotoxin subunit B"
                     /protein_id="HFO5822068.1"
                     /translation="LTSNALVGLSTCATSTECFGPDRKKN"
BASE COUNT          211 a          167 c          175 g          180 t
ORIGIN      
        1 gcgcagataa cggcagcagc tgaagatgcg gaaatacatc atgattatgc ccgggcatta
       61 atattgtggc agcatgcata tcatgctgcc acgctgactc tcaacaaaaa cctggcagtt
      121 gctaaaatcg acttctgtat taaacgtatc cggatgcatg aacaaatgag caggataatc
      181 cggaagactg gcacagacga aatactcttc cgtctgagta aacaccatca tttatatgag
      241 aaaaagaaaa caaaagaggg gtgagaaacc cattgtatgg ggaaaacttg ccatgctcac
      301 tgcgggggca tttttgtact ggctgttcta accgggtaac ccgttgatac acatagtaca
      361 ataatgtttt atgcaggctt acgcgccaga ctgccatcag tagtgcattg cgtgccagct
      421 cgcggacagg ggcagtgacc agccaccaca tgagtaagca tcatttcagc gccatcaggc
      481 agctcctgaa actcctgcga gttgaaaaag catcctcctg cattagcaat actggcttgt
      541 cagtgccagt gtgatataag acgtaatgtc aaagttcgtg gtgttgacat atttctgtcg
      601 caaccaaagt atgctcccgc ccagtatata tctgggcagg agcatcattt attagttctt
      661 tttcctgtcg gggccaaaac attctgtcga tgtggcgcag gttgataacc caacaagggc
      721 attggacgtt aat
//
LOCUS       DAZFLD010000663          732 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.666, whole genome shotgun sequence.
ACCESSION   DAZFLD010000663 DAZFLD010000000
VERSION     DAZFLD010000663.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..732
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.666"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>732
                     /locus_tag="ACHKBL_005375"
     CDS             <1..>732
                     /locus_tag="ACHKBL_005375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000842442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HFO5822069.1"
                     /translation="GKDARILKGSEKSGNWFAFGTPENGRPLLFAEGYATAASLHEAT
                     GLPVLMTIDADNMINVAKNARKIWTDSPFVFCADNDHQRKINKGVSSAKKAAASTHGE
                     VIIPTFTEAEKAQGLTDFNDLDASRGRDAFQRAMNAQLEYIGIPTPNNDTTDRREAIV
                     IGNLIFTPVKDEKPQVSPENRQSTAPEPAVSEQNHPYDIPAEETDNQAYEDDYAAYAS
                     LQSVTAGTASPNEPQVNAVSAIDSNA"
BASE COUNT          214 a          199 c          178 g          141 t
ORIGIN      
        1 gggaaggatg cccgcattct gaaaggctct gagaaaagcg gcaactggtt cgccttcgga
       61 accccggaaa atggccgtcc cctgttattt gcggaaggtt acgctactgc cgcctccctg
      121 cacgaagcaa ccggccttcc tgttctgatg acaattgatg ccgacaatat gattaatgtg
      181 gcaaaaaatg cccgcaaaat ctggactgac agtccctttg tcttctgtgc cgacaacgac
      241 catcaacgga aaattaataa aggtgtttca agtgcaaaaa aagctgccgc aagcacgcat
      301 ggcgaagtca ttattccgac ctttacggag gcggaaaaag cacagggact gaccgatttc
      361 aatgacctgg acgccagccg gggacgagac gcctttcagc gagccatgaa cgcacagtta
      421 gaatatatcg gtatccccac gcccaacaat gacactactg accgccgtga agctatcgtg
      481 atcggcaacc tgatattcac accggtaaaa gatgagaagc cacaagtgtc ccctgaaaac
      541 agacagagca cagcccctga accggcagtg tcggaacaga atcatcctta tgacatccct
      601 gcagaagaga cagacaatca ggcatatgaa gatgactatg cagcatatgc atccctgcag
      661 tcggtaactg cagggacagc ctccccgaat gaaccgcagg tgaatgctgt ttctgccatt
      721 gacagcaacg ca
//
LOCUS       DAZFLD010000664          730 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.667, whole genome shotgun sequence.
ACCESSION   DAZFLD010000664 DAZFLD010000000
VERSION     DAZFLD010000664.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..730
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.667"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..346)
                     /locus_tag="ACHKBL_005376"
     CDS             complement(<1..346)
                     /locus_tag="ACHKBL_005376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000197506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Imm57 family immunity protein"
                     /protein_id="HFO5822070.1"
                     /translation="MARLLTIVLFFSISANAVTVNQFQRELDLADKTILWALISASTQ
                     EGRKACSLSYFSCKAAEAELGLAYMAANDNKAFLTSLSRIMMYKIDAGLSESYTCYLL
                     SKGKIIRPYLKNL"
BASE COUNT          198 a          150 c          127 g          255 t
ORIGIN      
        1 ttagattctt cagatatggt cttatgatct ttcccttgct taataaatag catgtatagg
       61 attcggaaag tccagcatct attttgtaca tcattattct agataaggat gtgagaaatg
      121 ccttgttatc attagcagcc atatatgcca gtccgagttc agcctctgcg gctttacatg
      181 aaaaatagct aagtgaacac gcttttcgtc cctcttgggt actggctgat attagtgccc
      241 ataatattgt cttgtctgcc aaatccaact ctctctgaaa ttggtttaca gtaactgcat
      301 tcgcagaaat agagaaaaaa agcacaattg ttaataacct tgccatattt ctagtccgtc
      361 actgttaatg tgctatctgg ctctgaaaat attagctcat ttaatttttg catgtcatta
      421 gatgaaccag ttatacaacc ctcggaacag aatcgaggtc ctaaagatgg accaaaagcg
      481 tttaaacaac tatgtgttaa aaatccatca cgattatacg tttctgtatt tgctgacggt
      541 actaaacgta taccattagg ttttctattg gttgctccag gactgttaag caagcctcgt
      601 ccggtttgct tcattgagtt ctccacttta gactattgga ctatctttca acatggcatc
      661 gccttagtat accgctggtt ttccgctcat tgttaatttt ctctttcgtt catttcactt
      721 aattttcagt
//
LOCUS       DAZFLD010000665          730 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.668, whole genome shotgun sequence.
ACCESSION   DAZFLD010000665 DAZFLD010000000
VERSION     DAZFLD010000665.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..730
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.668"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..556)
                     /gene="fimE"
                     /locus_tag="ACHKBL_005377"
     CDS             complement(<1..556)
                     /gene="fimE"
                     /locus_tag="ACHKBL_005377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418733.1"
                     /note="upgraded from regulatory protein to switch DNA
                     invertase; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimE"
                     /protein_id="HFO5822071.1"
                     /translation="MSKRRYLTGKEVQAMMQAVCYGATGARDYCLILLAYRHGMRISE
                     LLDLHYQDLDLNEGRINIRRLKNGFSTVHPLRFDEREAVERWTQERANWKGADRTDAI
                     FISRRGSRLSRQQAYRIIRDAGIEAGTVTQTHPHMLRHACGYELAERGADTRLIQDYL
                     GHRNIRHTVRYTASNAARFAGLWER"
BASE COUNT          227 a          181 c          144 g          178 t
ORIGIN      
        1 ttctttccca taatccggca aaacgagcag cattactggc ggtataacgt acagtatggc
       61 gaatatttcg atgcccgaga taatcctgaa ttaaacgggt atcggcacca cgctccgcca
      121 gttcataacc gcaagcatgc cttaacatat gaggatgagt ctgcgttacg gttccagctt
      181 caatacctgc atcgcgaata atgcgatagg cctgctggcg agaaagccga ctcccgcggc
      241 gagaaataaa tatagcgtcg gtccggtcag cgcctttcca gttagcacgt tcctgggtcc
      301 agcgttccac ggcttcacgc tcatcaaaac gtaacgggtg aacggtagaa aatccgttct
      361 tcagtcggcg aatatttatt cgaccttcat taaggtcaag atcctgataa tgcagatcaa
      421 gcagttcact aatacgcatc ccatgccgat atgccaacag aataagacaa taatctctgg
      481 ctcccgttgc cccgtaacaa accgcctgca tcatggcctg aacttcttta ccggtaagat
      541 aacgacgttt actcacaata gtagtactcc taactgagat atatttgaat gaatacctat
      601 aggaaacctc aatcggtcaa aattagccca atagataaaa agataaccac acacgaagtg
      661 atgtggctat cagtcaatta cattaaatat catacaaaaa taaaatatca ctgatgtgac
      721 aacatctatt
//
LOCUS       DAZFLD010000666          727 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.669, whole genome shotgun sequence.
ACCESSION   DAZFLD010000666 DAZFLD010000000
VERSION     DAZFLD010000666.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..727
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.669"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            105..407
                     /locus_tag="ACHKBL_005378"
     CDS             105..407
                     /locus_tag="ACHKBL_005378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HFO5822072.1"
                     /translation="MIRDDKAEALENSGLWRRAAERWGELFRQAKTDEERHYITQRRA
                     QCTAKATRPKTTEWGHVCAVRAAATRTLKDMGIDQKKEDPLRGTKYRGETGRRDGS"
     gene            409..576
                     /locus_tag="ACHKBL_005379"
     CDS             409..576
                     /locus_tag="ACHKBL_005379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HFO5822073.1"
                     /translation="MDTITDKKAEQLESQGLWRRAAARWLDVMKEAHTDPQREHIARR
                     REICLANFRML"
BASE COUNT          235 a          150 c          211 g          131 t
ORIGIN      
        1 tattgcgtca gtctcatagt gacctttctt gttgtaggat gaatccctca tacaataagc
       61 cataagacaa attttgaaag gacagaatcg gcggggggta acagatgata cgtgatgaca
      121 aggctgaagc gctggagaat agcggcctct ggcggcgtgc tgctgaacgc tggggagaat
      181 tgttcagaca ggcaaaaact gatgaagaga ggcactatat tacccaacga agggcgcaat
      241 gcacggcaaa agccaccaga ccaaaaacaa cagagtgggg gcatgtatgc gccgtcaggg
      301 cagcagcgac cagaacgctt aaagatatgg ggattgacca gaaaaaagaa gatcctttac
      361 ggggcacgaa gtacaggggt gaaacagggc gaagagacgg aagttaatat ggacacaata
      421 actgataaaa aagccgaaca actggaatct cagggactat ggcgaagagc cgccgctcgt
      481 tggcttgacg taatgaaaga ggcccacacc gatccgcagc gagagcatat agccagacgg
      541 cgggaaatat gccttgcgaa cttcaggatg ctgtaaataa gactgttgac gatatgggaa
      601 tcagacggga tcggcgacat tacgggttat ggaaataaca gccaggtaaa ttggtgaata
      661 tacggcaggc tgcaatcaga gccgaacata gcggcctccg tcaccattat cagacagtag
      721 cggtagt
//
LOCUS       DAZFLD010000667          725 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.670, whole genome shotgun sequence.
ACCESSION   DAZFLD010000667 DAZFLD010000000
VERSION     DAZFLD010000667.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..725
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.670"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(52..285)
                     /locus_tag="ACHKBL_005380"
     CDS             complement(52..285)
                     /locus_tag="ACHKBL_005380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5822074.1"
                     /translation="MARSDYDFINLSLEHELNEWLAERGYAGLADNRNRLAEVVACKL
                     QDSFCMNVSWDALNAAYSEHPEWFSGLVSGDEN"
     gene            complement(380..>725)
                     /locus_tag="ACHKBL_005381"
     CDS             complement(380..>725)
                     /locus_tag="ACHKBL_005381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFO5822075.1"
                     /translation="FGCVYSLKASLSGVPDTYRYHLSHRIRRMVGSENTSLPYQQVAR
                     EVKAPRERLKYALEAGLLVTALDGLFWSGSQRIAADVLRLRKAGMPVVTTSVEVHDNL
                     TGTTRKIPAYHL"
BASE COUNT          175 a          225 c          158 g          167 t
ORIGIN      
        1 aagaaccccc tgccggcaat cacactgcct gatggcagca taatccactt attaattctc
       61 atccccggag acaagccctg aaaaccactc gggatgttca ctgtatgctg cgttcagagc
      121 atcccaggaa acgttcatac agaagctgtc ctgcaatttg caggcaacca cctctgccag
      181 gcggttccgg ttatccgcca gtccggcata acctctctct gccagccact cattcagttc
      241 atgttccaga gacagattga taaaatcgta atcggacctt gccattcccg tttccttaac
      301 acatgtcttc agactcaggg ataaagtata aaaatgtcgc cctgctgctg ccagagcaat
      361 attccccctg atggccgtat cagagatggt atgccggtat tttacgggtg gttcccgtca
      421 ggttatcgtg tacctccacg ctcgtagtca ccaccggcat tccggccttt ctcagcctca
      481 aaacatcagc tgcaatacgc tgactaccag accagaacag gccgtccagt gcagtcacca
      541 gtaaccccgc ctccagcgca tacttcagcc gttcacgggg cgctttcact tcccgggcaa
      601 cctgctgata tggcaatgat gtgttttcac tcccgaccat ccggcgaata cgatgagaca
      661 gatgataccg gtatgtatcc ggcacacctg aaaggctggc cttcaggctg tacacgcagc
      721 caaag
//
LOCUS       DAZFLD010000668          724 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.671, whole genome shotgun sequence.
ACCESSION   DAZFLD010000668 DAZFLD010000000
VERSION     DAZFLD010000668.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..724
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.671"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            153..629
                     /locus_tag="ACHKBL_005382"
     CDS             153..629
                     /locus_tag="ACHKBL_005382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001165712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR family transcriptional regulator"
                     /protein_id="HFO5822076.1"
                     /translation="MQIIIMTRDRYLEYGLMCMLNGYRLTTGRELFDAGKRRLPLPED
                     SYVILCDRNLERLTYCMFCGRRFLVIPVSSVRCLTDIRQAIRRGAWLFGHTARPLTRT
                     EMVVVFGVVFHEYGFTFLADQLGISMKTVCAHLYNAMEKNGLRGVSIKYLCSTADR"
BASE COUNT          174 a          139 c          215 g          196 t
ORIGIN      
        1 gaaggagaag gggacctgaa agagctgagt ctgtcagagc tggaggagcg ggcactgatg
       61 tatgcggcag acagtttccg ttatgaatga tgagtcagtt ataccggtaa tggtaaacgg
      121 agccggtatc cgggagataa gggtctgaga gtatgcagat tattattatg acccgtgaca
      181 gatatctgga atatggcctg atgtgtatgc tgaacggata caggctcacg acaggccgtg
      241 aactgtttga tgccggaaag cgccgtctgc cacttcccga agacagttat gtgattctct
      301 gtgaccgtaa tctggaaagg cttacatact gtatgttctg tgggcgtcgt tttcttgtca
      361 ttcctgtttc ctctgtgaga tgcctgacag atatcaggca ggccatccgt cgtggggcgt
      421 ggctgttcgg acatacggca agaccactga cccgtaccga gatggtggtg gtcttcggag
      481 ttgttttcca tgagtacggt tttacctttc tggcagacca gctggggata agcatgaaga
      541 cggtatgtgc gcatctttac aatgcgatgg agaaaaatgg tctgcgcggc gtcagtatta
      601 aatatctctg cagcaccgca gaccggtaaa tccgggataa ttaatgttat gtggccaggc
      661 gtgataaatt tcatgtggaa cgggtatgca ttttatttgt gataacagtt attttctgga
      721 ggga
//
LOCUS       DAZFLD010000669          722 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.672, whole genome shotgun sequence.
ACCESSION   DAZFLD010000669 DAZFLD010000000
VERSION     DAZFLD010000669.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 722)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 722)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..722
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.672"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..186
                     /locus_tag="ACHKBL_005383"
     CDS             <1..186
                     /locus_tag="ACHKBL_005383"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5822077.1"
                     /translation="LTSRHQEADCQSGQDKAFFHRVFTSAVINQRSMCRRRRGWLNEQ
                     LITRWRKKTPADMQALC"
     gene            complement(246..>722)
                     /locus_tag="ACHKBL_005384"
     CDS             complement(246..>722)
                     /locus_tag="ACHKBL_005384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001005016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HFO5822078.1"
                     /translation="RELYGEGRPPRVIDVGLLDDEGIRRYKQMAALMLMMKLRQKKVD
                     LMVHLDFLSQLIQIQETHTQVVTLFNYMVKECDSASPEFIRAMAERLPRHEDDLMTIA
                     ERLELAGIEKGRQEEREKALQENYASARRMQQMGISSEKIKEILQFSDDDLRNALR"
BASE COUNT          197 a          193 c          149 g          183 t
ORIGIN      
        1 ctgacatccc gccatcagga ggcagactgt cagtccggac aggataaagc gttttttcat
       61 cgggtattta cctctgcagt gataaatcag aggtcaatgt gcagaagaag acggggctgg
      121 ctcaatgaac aactcattac ccgctggcga aaaaaaacgc ctgcagacat gcaggcgctt
      181 tgttgacagg aaggaaagca acataacaaa tatgtcacaa tcctgcggtt aacataaatt
      241 accgattacc ggagagcatt acgcaaatca tcatccgaga actgcagtat ttctttgatt
      301 ttctctgacg atatacccat ctgctgcatg cgacgggccg atgcgtaatt ctcctgaaga
      361 gccttttctc tctcctcctg gcggcccttc tcgataccag ccagttccag tcgttcagcg
      421 atagtcatta gatcatcctc atgtcttggc agacgttctg ccatcgcccg tataaattca
      481 ggtgaagctg aatcacattc tttcaccata tagttaaaca atgtcaccac ctgagtgtgt
      541 gtttcctgta tctgaatcaa ctgtgacaaa aagtcaagat gaaccatcag atccactttc
      601 ttctgccgga gcttcatcat aagcatcagc gcagccatct gcttatagcg acggataccc
      661 tcatcatcaa gaagaccgac atcaatcacc ctgggtggcc tgccttctcc ataaagctcc
      721 cg
//
LOCUS       DAZFLD010000670          722 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.673, whole genome shotgun sequence.
ACCESSION   DAZFLD010000670 DAZFLD010000000
VERSION     DAZFLD010000670.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 722)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 722)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..722
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.673"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>722
                     /locus_tag="ACHKBL_005385"
     CDS             <1..>722
                     /locus_tag="ACHKBL_005385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPD38 domain-containing protein"
                     /protein_id="HFO5822079.1"
                     /translation="VAGEDDDGVNWYDKVPSHVKERNLVIMKSVFGGKAGEYWSIPLP
                     YGYNVFFLLGHTAEGVAAGDLTASRAAGNVVGGVLGAFSPIGSETSETLSGALLKNAA
                     PTILRPFANLAMNENFMGAQIYQENMPFGTPKPDSQLGRRSTPEAYKAFASWLNAFSG
                     GSQYRPGAVDVTPESLKYWIDYISGGTGRFISKTTDAAVKSLNGIDIPEQQVPFLGKI
                     SGEVMPYADQQKMYDRMTEVAQ"
BASE COUNT          161 a          165 c          230 g          166 t
ORIGIN      
        1 gtggcgggtg aggatgatga cggggttaac tggtatgaca aggtgccgtc tcatgtgaaa
       61 gagcgtaacc tcgtcattat gaaatcggtg ttcgggggca aggccggaga gtactggagt
      121 attcctctgc cttacgggta caacgttttc ttcctgctcg ggcataccgc tgaaggtgtg
      181 gcggcgggtg acctgacggc gtcccgtgct gccggtaatg ttgttggtgg tgtgcttggt
      241 gcattcagcc cgattggcag tgagacgtcg gaaacactgt ccggggcatt gctgaaaaat
      301 gcagcgccga ccattctgcg tccgtttgcg aaccttgcca tgaatgaaaa cttcatgggg
      361 gcgcagattt accaggagaa catgccgttt ggtacaccaa aacctgacag ccagctggga
      421 agacgttcaa cgccagaagc gtacaaggcg tttgcatcct ggctgaatgc gttctcaggt
      481 ggcagccagt accgtcccgg cgcggtggat gtcacgccgg aatcactgaa atactggatt
      541 gactatatct ctggtggtac agggcgcttc atttccaaaa ccacggatgc ggcggtgaaa
      601 tcgctgaatg gtattgatat accggagcag caggtgcctt tcctggggaa aatttcaggt
      661 gaggtgatgc cgtatgctga ccagcagaag atgtacgacc ggatgacaga ggttgcgcag
      721 ta
//
LOCUS       DAZFLD010000671          719 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.674, whole genome shotgun sequence.
ACCESSION   DAZFLD010000671 DAZFLD010000000
VERSION     DAZFLD010000671.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..719
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.674"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..259
                     /locus_tag="ACHKBL_005386"
     CDS             <1..259
                     /locus_tag="ACHKBL_005386"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5822080.1"
                     /translation="YKSVWRMHTYEYPTATISCLTKLNNENILNIITGIEVILLAAKK
                     PACKDRTDKIAEMLKIPFSVVKEFIDEQALLNKSNIQGTGS"
     gene            complement(306..458)
                     /locus_tag="ACHKBL_005387"
                     /pseudo
     CDS             complement(306..458)
                     /locus_tag="ACHKBL_005387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001422102.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
BASE COUNT          245 a          153 c          139 g          182 t
ORIGIN      
        1 ttataaatcc gtatggagaa tgcacacata cgaatatcca acagcaacca tcagttgttt
       61 gacaaaatta aataatgaaa atatattaaa tattattaca ggtatagaag taatactcct
      121 cgcagcaaaa aaacctgcat gtaaagacag aacagataaa attgctgaaa tgctgaaaat
      181 accattctct gtcgtcaaag aatttattga tgaacaggct ttattgaaca aatcaaacat
      241 tcagggaact ggcagttgaa caaaacgcca gtccgaagac tggcgcagtg ataaaacatt
      301 ctgcatcata ccacattctg ctcctgaaga tgatgcagac gatgctccca gtttcccggg
      361 taatacccgg gcgtcagcag tacgcggcgt cgctcattac agagcgagtt accaatcact
      421 acttctccgg ccacgccaca cagcgacagt tgtatatagg ggtttgggga gcaatgaaac
      481 agtaaacgcc attaagaggt taataaaaat agccaattat ttattaatca ataaattata
      541 tctattcatt gaacgctctc agctccccct tactggggtc cgcctggaat tcagaaaata
      601 aaccacgtta agactgtttt ttagccaaat gactttcagg gatcgcctga atggcgggac
      661 aacagcggtt ttggtgtccg aaatcgtaca attctgtaca tctacacaag ggaattatc
//
LOCUS       DAZFLD010000672          715 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.675, whole genome shotgun sequence.
ACCESSION   DAZFLD010000672 DAZFLD010000000
VERSION     DAZFLD010000672.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..715
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.675"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            341..>715
                     /gene="tssD"
                     /locus_tag="ACHKBL_005388"
     CDS             341..>715
                     /gene="tssD"
                     /locus_tag="ACHKBL_005388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tube protein TssD"
                     /protein_id="HFO5822081.1"
                     /translation="MSDIVYLRIIGEKQGYISSGCGTYASVGNCWQVGHEDEIFAFAL
                     TNAITSTGKGVNLQGLQFCKLIDKSSPLLSNAINQNERLFIEIDLYRINKSGRWERYY
                     YIQLRNASLTAIHVNISDNNLPT"
BASE COUNT          240 a          112 c          135 g          228 t
ORIGIN      
        1 tttggataaa aaaaaagcaa ttgtaagttt ttcatcacat ttaatagata ctttaagtct
       61 gataacatac tgttttaact agctatatgc cggatttgtc tgcctgctga aaactatgcc
      121 ttagccacca gcacaataat cttacaaaag gtcatataag aataaataga tgttaataat
      181 acaatgggtt tgtaacgcga gtatttaaat ataatattgc gagctaagtg aaaacgttaa
      241 gtaattgaaa taatctttca ttaaaagcgt ttcttattga tcgagaatta agttttctat
      301 ttaatgcaaa taagttaagg ataaactgga atgaaataaa gtgagcgata ttgtttacct
      361 gagaataatc ggtgagaagc agggctatat ttcttcaggc tgtgggacgt atgcgtcggt
      421 tggtaattgc tggcaggttg gccatgaaga tgaaattttt gcctttgcac tcaccaacgc
      481 cattaccagt actggtaaag gcgttaatct acaggggcta caattttgca aactcattga
      541 taaaagctca ccgctactgt ctaatgccat caatcagaat gagcggttat ttattgaaat
      601 cgatttgtat cgtataaata aaagcgggcg ctgggaacgg tattattata ttcagctaag
      661 aaatgcttca ttaactgcta ttcatgtaaa catttctgac aataatcttc ctacc
//
LOCUS       DAZFLD010000673          714 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.676, whole genome shotgun sequence.
ACCESSION   DAZFLD010000673 DAZFLD010000000
VERSION     DAZFLD010000673.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..714
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.676"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            134..>714
                     /locus_tag="ACHKBL_005389"
     CDS             134..>714
                     /locus_tag="ACHKBL_005389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000781912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5822082.1"
                     /translation="MKMIFTGKVSGEKTVLTAGARHTVKAQAGEQYGLVDEVTGLVPD
                     GVEADRSGDDLILRKKEDDTEIRIEGFWEECQPGETQCTAVFNVVGENGQVTEAVLTQ
                     DGPVLENFTAGQSGTLAEGINNGIIWGGIAFGAGLAAIALAAGGGGGSSRHKRATEGE
                     QKPVQPEGGQEAGKTEVSSTGSTEAGSGSSSGQV"
BASE COUNT          198 a          127 c          224 g          165 t
ORIGIN      
        1 aaaaagcgga atgattaagg aggtccggtc agagaaataa attatctgta atctgattcg
       61 tttttattta atgactccct ttagcggata aaacataaga gcatactggt tttattaacc
      121 tgaggatact tatatgaaaa tgattttcac cggcaaggtc agtggagaaa aaacagtact
      181 gacagccgga gcccgtcata ctgtaaaggc acaggccggg gaacagtatg gactggttga
      241 tgaggtgaca ggtctggtcc cggatggtgt ggaagcggac cgttctggtg atgatttaat
      301 tctgcgtaaa aaggaagatg atacagaaat ccggattgag ggattctggg aagaatgcca
      361 gccgggtgaa acacagtgta cagccgtgtt taatgtggtg ggtgaaaatg gccaggttac
      421 tgaagctgtc ctgacccagg atggtcctgt actggagaat ttcactgcag ggcaaagtgg
      481 cacactggct gaaggtatca ataacgggat tatctggggt gggattgctt ttggtgcagg
      541 gctggcggct attgcactgg ctgccggagg cggtggtgga agcagtcgtc ataagcgtgc
      601 aactgaaggt gagcagaagc cggttcagcc tgaaggcggt caggaggctg gaaaaacaga
      661 agtatcatcc acaggctcaa ctgaggcggg aagtggaagc tcttccggcc aggt
//
LOCUS       DAZFLD010000674          712 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.677, whole genome shotgun sequence.
ACCESSION   DAZFLD010000674 DAZFLD010000000
VERSION     DAZFLD010000674.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..712
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.677"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..175
                     /gene="cdiI"
                     /locus_tag="ACHKBL_005390"
     CDS             <1..175
                     /gene="cdiI"
                     /locus_tag="ACHKBL_005390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001046155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ribonuclease toxin immunity protein CdiI"
                     /protein_id="HFO5822083.1"
                     /translation="HFEGVEFAVGYPPDEDDIVIVSEETCFEYVRLACEKYLQLHPED
                     TEKVNKLLSKIPS"
BASE COUNT          210 a          168 c          145 g          189 t
ORIGIN      
        1 acattttgaa ggtgttgaat ttgctgtcgg ctatccccca gatgaagatg atatagtgat
       61 cgtaagcgaa gaaacctgtt ttgaatatgt tcgtctggcc tgtgaaaaat atcttcagtt
      121 acaccctgaa gatactgaaa aagtaaacaa attactgtct aaaatcccat cctgagtaaa
      181 gattccggcc actgcgccgg gatctttctt taaccgtcag gctcatccac aatgtccatc
      241 cccacgctta atcaccggca catctctaat gtcgttaagc gcaccgggga aggcgttaaa
      301 ccacgtgagc atctgcggcc tgttgacata cgttgtgtcg ttacggggaa gtcatcttca
      361 cagcgatttt cagtcagtta gcattaccga cattcattca tcaaattacc ggagataatt
      421 gatgtcaaaa ccgtaagcgt aaactgaccg ccgtatgtag ccatcagacg agaattggta
      481 acttagacgc ccatctgata tagacggaca tctaagtatg gaattacagg actggcgaaa
      541 agaacctcgt aaaaactatt cgaatgaatt caaacttcgt atggtggaac tggcatcaca
      601 acctggaggt ttatcccaag aatattgcct ttgatatgct ggcacataca atccttgacc
      661 attctcacag atatactccc acacactttt gggttgcgcc ccgaaaacat ct
//
LOCUS       DAZFLD010000675          712 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.678, whole genome shotgun sequence.
ACCESSION   DAZFLD010000675 DAZFLD010000000
VERSION     DAZFLD010000675.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..712
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.678"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(98..>712)
                     /locus_tag="ACHKBL_005391"
     CDS             complement(98..>712)
                     /locus_tag="ACHKBL_005391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO5822084.1"
                     /translation="PALVYTPEDIIFLRMYDPRQQIYGLPDYIGGIHSALLNSEASLF
                     RRRYYNNGAHMGFLLYTNDPGITTEMEDEIREKVAESKGLGNFRNLYINIPKGSPDGV
                     KIIPIGEVSAKDEFSNIKGISAQDVFTAHRFPAGLAGIIPTNGATIPNPETTRETYRK
                     DEVIPLQRKIMNAVNSDPDIPEHLYLCFDVDAGHVAEEKAVPVG"
BASE COUNT          159 a          208 c          177 g          168 t
ORIGIN      
        1 tgtcttccgg atgacagcag gtgcgccaca gtcaggacac cgggctttaa aaatccgcat
       61 cttacctccc gataacacgt attccgggga ggacatttta cccgaccggc accgcttttt
      121 cttccgccac atgcccggca tccacatcaa aacaaagata aaggtgttcc ggtatatcgg
      181 gatcgctgtt cacggcgttc atgattttac gctgaagcgg tatcacctca tccttgcggt
      241 acgtttcacg cgtcgtttcc gggttaggga tggtggcccc gttcgtgggg ataatccccg
      301 caagaccggc aggaaagcgg tgtgccgtaa acacatcctg ggcgctgatc cccttgatgt
      361 ttgaaaattc atccttggcg ctcacctcac caatcggaat aattttcacc ccatccggcg
      421 aacctttggg gatgttgatg tacagattgc ggaaatttcc cagcccctta gactccgcca
      481 ccttctcgcg gatctcatcc tccatctccg tggtgatccc agggtcgttc gtatacagca
      541 aaaagcccat atgcgccccg ttgttgtagt aacggcgacg aaaaaggctg gcctcgctgt
      601 tcagcagcgc cgagtggatg ccaccgatat aatccggcag cccgtaaatt tgctgacgcg
      661 gatcatacat ccgcagaaag ataatatctt ccggcgtata gaccagcgcg gg
//
LOCUS       DAZFLD010000676          705 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.679, whole genome shotgun sequence.
ACCESSION   DAZFLD010000676 DAZFLD010000000
VERSION     DAZFLD010000676.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 705)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 705)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..705
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.679"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            288..554
                     /locus_tag="ACHKBL_005392"
                     /pseudo
     CDS             288..554
                     /locus_tag="ACHKBL_005392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709438.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          226 a          149 c          131 g          199 t
ORIGIN      
        1 aacatcacac cgctatagac cgctaacaat tttgatgcct gtaatttcaa tcatcatcct
       61 gtcgttattt cagcaccatc acaccaaaaa tcaactaaca tgttgtctta gccgactatt
      121 cacaatgcat tggaatgcag ataaagataa atttttatcc acatgaagaa ttactgtcga
      181 tgatattgta aacaatattc atcccgggca ataacaagac ttgatacaca acatcacgac
      241 ttttatgctt ttcagattgt gtcccatccg ttttacaggc tcagaaaatg aacgacagat
      301 tacgctttga tgtgaacgat aaccaaggcc gtttcgtatt tccgaatacc aggtttggcc
      361 cattactgga ggagtttgaa gaagtgcatg acgcatacgg cactgatgaa atcagcgaga
      421 cgagttatat caacaaactc aggtagcttg cccaacggga gcctgacttt attgatatac
      481 acgctcatct ggcatatgca tttctggagc agccaaggtg ggcaatattt ttatttattt
      541 ctgtgaaaag cctgctaaag atcccatgct tctatattaa tgactggaac aatactagga
      601 agcacattaa gaaaaaataa aggaagccga tggctaccac gtttatcgtt tagaagcgtg
      661 agctcttcat tattaagccg gacacttaca aaatgaagtt gcttc
//
LOCUS       DAZFLD010000677          697 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.680, whole genome shotgun sequence.
ACCESSION   DAZFLD010000677 DAZFLD010000000
VERSION     DAZFLD010000677.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..697
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.680"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>697
                     /gene="traJ"
                     /locus_tag="ACHKBL_005393"
     CDS             <1..>697
                     /gene="traJ"
                     /locus_tag="ACHKBL_005393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001480051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="plasmid transfer ATPase TraJ"
                     /protein_id="HFO5822085.1"
                     /translation="GRYGLQRGQRVRLRCHLIQGTSATEEKAISVTMRVIPSDIPDIL
                     TMNIEPDLLDAMLRKTGLGFICGETGSGKSTLAAALYRHILKNFPDRKIVTYEDPVEY
                     ILGGENDLLPPHQAEIGRDVESFAAGLRSAVRRNPDIIGIGEIRDYETADAAVQAGNT
                     GHFCIGTMHTKSPGETFARLLGLFPPEIRDSMAAATLSLVQFILVQVLVRTNDGGRQA
                     VREYIVMTDELRNT"
BASE COUNT          170 a          192 c          185 g          150 t
ORIGIN      
        1 ccggtcgcta tggcctgcag cgaggtcagc gtgttcgcct gcgctgccac ctgatacagg
       61 gaacatcggc aacagaagaa aaagccattt ccgtcaccat gcgggtgatc ccgtcagata
      121 ttccggacat cctcactatg aatattgaac cggatctgct ggatgccatg ctcagaaaaa
      181 ccggcctggg cttcatctgc ggagaaaccg gctccgggaa atccacactg gcggcggcac
      241 tgtatcgcca cattctgaaa aatttccccg atcgcaaaat agtgacctat gaagatccgg
      301 tggaatacat tctgggcgga gagaatgacc ttctgcctcc ccatcaggcc gaaatcgggc
      361 gcgatgtgga aagttttgct gcaggacttc gctctgccgt tcgccgtaat ccggacatca
      421 ttggtatcgg tgaaatcagg gattatgaaa cagcagatgc cgcagtccag gcgggtaata
      481 ccggacactt ttgtatcggc accatgcaca caaaatcacc cggagaaacc tttgcccgcc
      541 tgctgggatt gtttcctcct gaaatcaggg attcgatggc tgcagccacg ctttccctcg
      601 tacagttcat tctggtgcag gtactggtca gaacaaatga cggcggcagg caggctgtcc
      661 gggaatacat cgtcatgact gacgaactgc gcaatac
//
LOCUS       DAZFLD010000678          692 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.681, whole genome shotgun sequence.
ACCESSION   DAZFLD010000678 DAZFLD010000000
VERSION     DAZFLD010000678.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..692
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.681"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..381
                     /locus_tag="ACHKBL_005394"
     CDS             <1..381
                     /locus_tag="ACHKBL_005394"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5822086.1"
                     /translation="LCFLMLLLSGCNDKAKDFIGGWTQESNEEYPVYITINYDSGIYH
                     VDVKKLDTYLVEKKKADAFDDFMWGKKSKNESENLTFDDCYKVFNFEAKAISSSVLEG
                     KGISVRMDGDRLIFDGKRFVRKKS"
     gene            407..>692
                     /locus_tag="ACHKBL_005395"
     CDS             407..>692
                     /locus_tag="ACHKBL_005395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFO5822087.1"
                     /translation="MQVNVAETLFRTIDTATQSQMANMNTVMSVMGIVIGGIWMTYIL
                     MKSLYWYFEGLTTVIQDAFFTIFKAFAIMMASLTISWYTSTVIPPVLEFPL"
BASE COUNT          244 a           74 c          146 g          228 t
ORIGIN      
        1 ttgtgctttt taatgttgct tttgtctgga tgtaatgata aagcaaaaga ctttatagga
       61 gggtggactc aggaatcaaa tgaagaatat ccagtatata taaccataaa ctatgatagc
      121 ggaatttatc atgtggatgt taaaaaacta gatacttatc tggtagaaaa gaaaaaagct
      181 gatgcatttg atgatttcat gtgggggaaa aaaagtaaga atgaatctga aaatttaaca
      241 tttgatgatt gttataaagt gttcaatttt gaagctaaag caataagtag ttcggtatta
      301 gaaggtaagg gtatatctgt aaggatggat ggtgatagat taatttttga tggaaaaaga
      361 tttgtaagga aaaaatcata atgtgacata gctaaaggat ttttatatgc aggtaaatgt
      421 tgcggaaacg ctgtttagaa caattgatac cgcgacacaa tctcagatgg caaatatgaa
      481 tactgtcatg tctgttatgg ggattgtgat aggcggtata tggatgacat atatattaat
      541 gaagtcatta tattggtatt ttgagggctt aacaactgta atacaggacg cattttttac
      601 aatatttaaa gcatttgcaa tcatgatggc atcattgact atatcatggt atacatcaac
      661 agttattcct ccagttttag aattcccttt at
//
LOCUS       DAZFLD010000679          687 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.682, whole genome shotgun sequence.
ACCESSION   DAZFLD010000679 DAZFLD010000000
VERSION     DAZFLD010000679.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..687
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.682"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..387
                     /locus_tag="ACHKBL_005396"
     CDS             <1..387
                     /locus_tag="ACHKBL_005396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO5822088.1"
                     /translation="VLSSTQTLNAYNKFVMFILMNYTIEYYSEEVRLEVDQLPLSMRA
                     RYQHLIERMKVYGSNLGEPHTSAFGDGLFELRIKGCDGIARVFYCTLAGKRIIMLHSF
                     IKKTQKTPPAELKKAETRMKEVKHDW"
     gene            377..685
                     /locus_tag="ACHKBL_005397"
     CDS             377..685
                     /locus_tag="ACHKBL_005397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO5822089.1"
                     /translation="MTGKRNPPTMTHDEMAAKWMEDPAFKAEYDAIADEFALLDEMLA
                     ARKEAGLTQAEVAERMGTKATAITRMESNLASGISGPSFATLKKFARATGKKLQIRFV
                     "
BASE COUNT          213 a          163 c          157 g          154 t
ORIGIN      
        1 gtgttgtcgt caacacagac cttgaatgca tataacaaat ttgttatgtt cattctcatg
       61 aactacacta tcgaatacta cagtgaagag gtccggctgg aagtcgatca gcttccattg
      121 agtatgcgtg cccgatacca acatctcatt gaacgtatga aggtatacgg cagcaatctc
      181 ggagaacctc acaccagcgc cttcggtgac ggacttttcg agcttcggat taaaggctgt
      241 gatggcatcg cacgcgtttt ttactgcact ttggcaggaa aacgcatcat catgctgcac
      301 agtttcatca agaagactca gaaaacccca ccggctgaac tcaaaaaagc tgaaaccaga
      361 atgaaggagg ttaagcatga ctggtaaacg taacccacca accatgacgc acgatgaaat
      421 ggcagctaaa tggatggagg atcctgcttt taaagcagaa tacgacgcca tcgccgatga
      481 attcgcactg cttgatgaaa tgctggcagc acgaaaagaa gctggcttaa cccaggctga
      541 agttgctgaa cgtatgggaa caaaagcgac tgcgatcacc agaatggaaa gcaatcttgc
      601 atcaggtata agtggcccat catttgccac actgaaaaaa ttcgcccgtg ctacagggaa
      661 aaaactccag atccgcttcg tttaact
//
LOCUS       DAZFLD010000680          684 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.683, whole genome shotgun sequence.
ACCESSION   DAZFLD010000680 DAZFLD010000000
VERSION     DAZFLD010000680.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..684
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.683"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            9..>684
                     /gene="yeeN"
                     /locus_tag="ACHKBL_005398"
     CDS             9..>684
                     /gene="yeeN"
                     /locus_tag="ACHKBL_005398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFO5822090.1"
                     /translation="MGRKWANIVAKKTAKDGATSKIYAKFGVEIYAAAKQGEPDPELN
                     TSLKFVIERAKQAQVPKHVIDKAIDKAKGGGDETFVQGRYEGFGPNGSMIIAETLTSN
                     VNRTIANVRTIFNKKGGNIGAAGSVSYMFDNTGVIVFKGTDPDHIFEILLEAEVDVRD
                     VTEEEGNIVIYTEPTDLHKGIAALKAAGITEFSTTELEMIAQSEVELSPEDLEIFEGL
                     VDALEDD"
BASE COUNT          223 a          121 c          153 g          187 t
ORIGIN      
        1 tcattccagt gggacgtaaa tgggccaata ttgttgctaa aaaaacggct aaagacggtg
       61 caacgtctaa aatttatgca aaattcggtg tagaaatcta tgctgctgcc aaacaaggtg
      121 aacccgatcc agaattaaac acatctttaa aattcgttat tgaacgtgca aagcaggcac
      181 aagttccaaa gcacgttatt gataaagcaa ttgataaagc caaaggcggc ggagatgaaa
      241 cgttcgtaca gggacgttat gaaggctttg gtcctaatgg ctcaatgatt attgctgaga
      301 cattgacttc aaatgttaac cgtacgattg ctaacgttcg cacaattttc aataaaaaag
      361 gcggcaatat cggagcggca ggttctgtca gctatatgtt tgacaatacg ggtgtgattg
      421 tatttaaagg aacagaccct gaccatattt ttgaaatttt acttgaagct gaagttgatg
      481 ttcgtgatgt gactgaagaa gaaggtaaca ttgttattta tactgaacct accgaccttc
      541 ataaaggaat cgcggctcta aaagcagctg gaatcactga gttctcaaca acagaattag
      601 aaatgattgc tcaatctgaa gttgagcttt ccccagaaga tttagaaatc tttgaagggc
      661 ttgttgatgc ccttgaagat gacg
//
LOCUS       DAZFLD010000681          681 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.684, whole genome shotgun sequence.
ACCESSION   DAZFLD010000681 DAZFLD010000000
VERSION     DAZFLD010000681.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 681)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 681)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..681
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.684"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>681
                     /locus_tag="ACHKBL_005399"
     CDS             <1..>681
                     /locus_tag="ACHKBL_005399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ArdC family protein"
                     /protein_id="HFO5822091.1"
                     /translation="LLWLAADEAGYVSNDWLTYRQAQQLGGHVRKGEVATLAVIYKDW
                     KKQAVDEHGNRLTDSEGEPLMEIVPVLKSNPLFNVGQCDGLPEHLMVKAEGSADALPA
                     DTFSERVLQVLNGSGVSVSSLPQDRAFYRPDLDRIVMPLSSQFDQVADYWTTLLHELV
                     HSTGHEKRLRRVGISTFTGRNSPEYAFEELIAEMGSAFLCAHLGIAGDVQHESYIDGW
                     LKVLKSDK"
BASE COUNT          155 a          157 c          221 g          148 t
ORIGIN      
        1 gttgctgtgg ctggccgcgg atgaagccgg gtatgtcagc aatgactggc tgacgtaccg
       61 tcaggcgcaa caactgggtg gccacgtccg taaaggggag gtggccacgc tggcggtgat
      121 ttacaaagac tggaaaaaac aggctgtgga tgaacacggt aaccggctga cggacagtga
      181 gggggagcct ctcatggaaa tcgtaccggt actgaaatcg aatcctctgt ttaatgtggg
      241 gcagtgtgac ggtttacctg aacatctgat ggtgaaggcg gaaggcagcg cggatgcact
      301 gccggcagat accttcagtg agcgggtgct gcaggtactg aatggcagcg gcgtcagcgt
      361 ctcgtcactt ccacaggaca gggcattcta ccggcctgat cttgaccgta tcgtgatgcc
      421 actgtcgtcg cagtttgacc aggtggctga ttactggacc accctgctgc atgagctggt
      481 acacagtacc ggtcatgaaa agcgcctgcg gcgggtgggg ataagtacct ttaccggtcg
      541 caacagcccg gaatatgcgt ttgaagagct gattgccgag atgggcagtg cttttctgtg
      601 tgcacatctc gggattgccg gtgacgtaca gcacgaaagt tatattgacg gatggcttaa
      661 ggttctgaag tctgacaaaa a
//
LOCUS       DAZFLD010000682          679 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.685, whole genome shotgun sequence.
ACCESSION   DAZFLD010000682 DAZFLD010000000
VERSION     DAZFLD010000682.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..679
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.685"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            309..>679
                     /locus_tag="ACHKBL_005400"
     CDS             309..>679
                     /locus_tag="ACHKBL_005400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization protein"
                     /protein_id="HFO5822092.1"
                     /translation="MKSAPNLKKQPRGKKHADTEVIIFAGSDAWAHAKQWLEQDGKLA
                     GDKTPPVVLADEQLKDIGNLQIVPDGRKSARIYKAGHLDQVMVKGIGQKLAAAGVQDA
                     DYYPEGMHHNERQNWRNYLET"
BASE COUNT          225 a          145 c          181 g          128 t
ORIGIN      
        1 cgcatgattg aacgtaacca cgaagaaatt gacgaccatg acctttctgt tattgcggga
       61 ctgattaaac aactttccag caatgtggct gtatggttaa tgacggagga ggaaaaacgc
      121 ggtgaacaat aacggcgaaa aataaacggg aaataaagat taagacgact ccttcaggag
      181 attaattcaa tggcatcaaa aaatactggc ttaactgcca gtagcaaaac tcaccccgaa
      241 tttatgtgcg gggatattta ccgcaatgaa tacggtagcc acatgacgat gatcagcaga
      301 ggtaagaaat gaaaagcgca ccgaacttaa aaaaacagcc tcgagggaaa aaacacgccg
      361 atacggaagt gattattttc gctggcagtg atgcgtgggc gcacgcgaaa cagtggctgg
      421 agcaggacgg aaaacttgcc ggagataaaa caccgcccgt cgtgctggct gatgagcaac
      481 ttaaagatat tggcaatctg caaattgtac ccgacgggcg caaatcagcg cgtatataca
      541 aggccggaca cctcgatcag gtgatggtca aaggcatagg tcagaagctg gcggcggcag
      601 gcgtacagga cgcagactat taccccgaag gtatgcacca taacgagcgg cagaactggc
      661 gtaactatct ggagactga
//
LOCUS       DAZFLD010000683          679 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.686, whole genome shotgun sequence.
ACCESSION   DAZFLD010000683 DAZFLD010000000
VERSION     DAZFLD010000683.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..679
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.686"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>679
                     /locus_tag="ACHKBL_005401"
     CDS             <1..>679
                     /locus_tag="ACHKBL_005401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000097914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system domain-containing
                     protein"
                     /protein_id="HFO5822093.1"
                     /translation="GSAVADWPEAEKPSFAGLCSALENRLARSGGMEALVPQNSSPRE
                     TGRESAPPDSPQLAAVTSGRDLLDQARLLSRWLSGQPQGWLASHRLMKTVRWDTVDQL
                     PSLDSSGRTRLVPPKTGYRAQLKRLWLQKNWVELIELASQMYCEGVNHFWLDLQWYLW
                     QGLSHAGQPWDSWSDAVLSDLRLLLKRLPGLEGLAWNDGTPFADEVTLAWIAEKVNEE
                     DVMYGDEA"
BASE COUNT          128 a          190 c          218 g          143 t
ORIGIN      
        1 ggatccgccg tggcagactg gccggaagcg gagaagccgt cttttgcggg gctttgttcc
       61 gcgcttgaga accgcctggc acgttcaggg ggcatggagg cactggtccc ccagaacagc
      121 agtccccggg agaccgggcg tgaatcagct cccccggact caccacaact ggcagcggtg
      181 acctcggggc gcgacttact cgaccaggcc aggttacttt cccgctggct gagcgggcag
      241 ccgcagggct ggctggcttc tcaccggctg atgaagacgg tgcggtggga tacggtcgat
      301 cagctccctt cgctggacag tagcggacgc acccgcctgg tccccccgaa aaccgggtat
      361 cgcgctcagc ttaagcgact ctggcttcag aaaaactggg ttgagctgat tgaactggcc
      421 agtcagatgt attgcgaagg tgtgaatcat ttctggctcg atcttcagtg gtatctctgg
      481 cagggactca gtcatgctgg tcagccctgg gattcctggt ctgacgctgt cctgtcagat
      541 ctgcggctgt tgcttaaacg tctcccgggc cttgaaggac tggcatggaa tgacggtact
      601 ccctttgctg acgaggtcac cctcgcctgg atcgctgaaa aagtgaatga agaagatgtg
      661 atgtacggcg atgaagctg
//
LOCUS       DAZFLD010000684          677 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.687, whole genome shotgun sequence.
ACCESSION   DAZFLD010000684 DAZFLD010000000
VERSION     DAZFLD010000684.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..677
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.687"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..615)
                     /locus_tag="ACHKBL_005402"
     CDS             complement(<1..615)
                     /locus_tag="ACHKBL_005402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HFO5822094.1"
                     /translation="MNIRNNHLPGTETLFSETETANSKQLAVLKTNFPQCFDKNGAFI
                     QEKLLEIIRASNVEISKESYCLNWLGKSYARLLANLPPTTLLSEDKDHNRREENKNSQ
                     NLLIKGDNLEVLKHMVNAYSEKVKMIYIDPPYNTGSDGFAYNDDRKFTPEQLSDLAGI
                     DLDEAKRILEFTTNGSSSHSAWLTFIYPRLYIARELLREDGVIFI"
BASE COUNT          196 a          133 c          139 g          209 t
ORIGIN      
        1 aataaatatc acaccatctt cacgtagaag ctcacgagca atatataaac gaggataaat
       61 gaacgttaac catgcgctat ggctgcttga accattcgtg gtaaattcaa gtatacgttt
      121 agcctcatca agatcgatac ctgccagatc agaaagctgt tctggtgtaa atttgcgatc
      181 atcattatag gcgaacccat cagaacccgt gttatatggc ggatcgatat aaatcatttt
      241 aaccttttca gaataagcat taaccatatg ctttaatacc tcaaggttat cccctttgat
      301 taacagattt tgactgttct tgttctcttc acgtctgtta tggtctttat cttctgacag
      361 caatgtcgtt ggtggtaggt tagccagcag gcgagcatag gatttgccaa gccagtttaa
      421 gcagtatgac tctttagaga tttcaacatt tgatgctctg atgatctcaa gcagcttttc
      481 ctgaatgaaa gctccgtttt tatcgaaaca ctgtggaaag ttagttttca aaacagcaag
      541 ttgtttactg ttcgcagttt cagtttcaga aaaaagagtt tctgttcccg gtagatgatt
      601 gttgcgtata ttcatacagc acctccagaa agcacggagg tagaggtact tgacggagag
      661 agttcaggcg agcatat
//
LOCUS       DAZFLD010000685          676 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.688, whole genome shotgun sequence.
ACCESSION   DAZFLD010000685 DAZFLD010000000
VERSION     DAZFLD010000685.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 676)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 676)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..676
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.688"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..146)
                     /locus_tag="ACHKBL_005403"
     CDS             complement(<1..146)
                     /locus_tag="ACHKBL_005403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5822095.1"
                     /translation="MKRILFFLLMIFVSFGVIADCETQAKDQDCFTIFTKGTIFSAFP
                     VLNN"
     gene            complement(143..>676)
                     /locus_tag="ACHKBL_005404"
     CDS             complement(143..>676)
                     /locus_tag="ACHKBL_005404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HFO5822096.1"
                     /translation="IGLKGGWNFYQYPLNPVQYIDSMGLASKYGHLNNGGYGARPNKP
                     PTPDPSKLPDIAKQLRLPYPIDQASSAPNVFKTFFRALSPYDYTLYCRKWVKPNLTCT
                     PQDDPQYPGMDTKTASDYLPQTNWPTTQLPPGYTCAEPYLFPDINKPDGPATAGIDDL
                     GEILAKMKQRTSRGIRK"
BASE COUNT          179 a          140 c          133 g          224 t
ORIGIN      
        1 ttattattta agacaggaaa cgcggaaaaa atagtaccct tagtgaaaat ggtaaagcaa
       61 tcttgatctt tagcttgtgt ttcgcaatca gctataacac caaaactaac aaatatcatc
      121 agcaaaaaga acaaaattct tttcattttc ttattcctct cgatgttctc tgtttcattt
      181 tagctaaaat ttcacccaaa tcatctatcc ctgctgttgc tggcccatcg ggtttattaa
      241 tgtctgggaa taaatagggt tctgcacaag tatatcctgg tggtaattga gttgttggcc
      301 aatttgtctg tggcaggtaa tcacttgctg tctttgtatc catccctgga tactggggat
      361 catcctgtgg cgtacaagtc agatttggtt ttacccactt cctgcaatac agtgtgtagt
      421 cgtaagggct taatgctctg aagaatgttt tgaaaacatt aggcgcacta ctggcctggt
      481 caataggata tggcagtctt aattgtttcg ctatgtctgg caatttactt ggatcgggcg
      541 taggcggttt gttgggtctc gctccatatc cgccattatt taagtgtcca tattttgatg
      601 ccagtcccat tgaatctata tactgaaccg gattcaacgg atactgataa aaattccatc
      661 cccccttcag cccaat
//
LOCUS       DAZFLD010000686          673 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.689, whole genome shotgun sequence.
ACCESSION   DAZFLD010000686 DAZFLD010000000
VERSION     DAZFLD010000686.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..673
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.689"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            147..578
                     /locus_tag="ACHKBL_005405"
     CDS             147..578
                     /locus_tag="ACHKBL_005405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5822097.1"
                     /translation="MKKIDSLRISKVAIISVSLLFLSACSTNNIENMPRDARVKFALM
                     RAYSDNKFDNSTVIGEVLGLSCARQLGKTLVTPLPGGSAIATSSEGDITSESDAIQDM
                     RYKAAMMGGDAIVNVVCKSGGVDWIHNCWSTVKCVGDVIKK"
BASE COUNT          211 a          104 c          168 g          190 t
ORIGIN      
        1 caaattttga aaggacagaa tcggcggggt acagatgata cgtgatgaca aggctgaagc
       61 gctggaagca caagggttat gcgatgggtt gcggcaatat gaggcaaata tgaatattca
      121 aattgttgtg ttgtgaggta cgcaagatga aaaaaattga ttctttacga ataagtaaag
      181 tagctattat atcagtatct ttattatttc ttagcgcgtg ttctacaaat aatattgaga
      241 atatgccgag agatgcgcgt gtaaaatttg ctctaatgag ggcttattca gataacaagt
      301 ttgataattc aacagttatc ggcgaagtgt taggattgag ttgcgccaga caacttggaa
      361 aaacattagt aacaccattg ccagggggaa gcgctattgc tacatcctct gagggcgaca
      421 ttacttctga gtcggatgca atacaggaca tgcgatataa agccgcaatg atgggaggtg
      481 atgccatagt gaacgttgta tgtaaatcag gtggtgttga ctggattcat aactgttggt
      541 caactgttaa gtgcgttggt gatgtaataa aaaaataaca gttgccatac tgaaggtatt
      601 gattaataca tggaaactgc aattacgacg tgtgattgca gcttaccgtt agctacccct
      661 gttacaattg ccc
//
LOCUS       DAZFLD010000687          671 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.690, whole genome shotgun sequence.
ACCESSION   DAZFLD010000687 DAZFLD010000000
VERSION     DAZFLD010000687.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..671
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.690"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..309
                     /locus_tag="ACHKBL_005406"
     CDS             <1..309
                     /locus_tag="ACHKBL_005406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5822098.1"
                     /translation="PQPGDELEITVVSPDRTTTAIITDILNGRPDSTNRSWLVAVRPR
                     RPPGEPEPLTPAGKIMRHYHEEQAHKHNAELTRTLASINRKAAIARYVHSAAGRQSRR
                     "
     gene            358..528
                     /locus_tag="ACHKBL_005407"
     CDS             358..528
                     /locus_tag="ACHKBL_005407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5822099.1"
                     /translation="MNSTRRISDRELTKMIESAQQLLSRQNPVVEETWWENLLAALTE
                     LRERRNNEGAAK"
BASE COUNT          209 a          198 c          178 g           86 t
ORIGIN      
        1 ccacaaccgg gcgatgagct ggaaatcacg gtcgtcagtc ccgatcgcac caccacggcc
       61 atcatcaccg acatactcaa cggacgaccg gacagcacca accgcagctg gcttgtcgcc
      121 gttcgtccgc gccgtccgcc cggagagccg gagccgctca cgcctgccgg aaaaatcatg
      181 cggcactacc acgaagagca ggcacacaaa cacaacgcag aactgaccag aacactggca
      241 tcgattaacc gcaaagcggc aattgcccgc tatgtgcatt cagccgctgg cagacaatca
      301 cgcaggtaaa caaagaccat gaacataacc agaaacagcc aactaaagga gcgcaccatg
      361 aacagcacca ggaggatcag cgacagagag cttacgaaaa tgattgagtc ggcgcagcag
      421 ctgctttcac gacaaaaccc ggtcgtggaa gaaacatggt gggagaattt actggcagca
      481 ctgaccgaac tccgggaacg ccggaacaac gaaggagccg ccaaatgaag accccaggaa
      541 tgaccggaaa ggaaatcatt ttacgctacc tggaaacgca cgcacgtttc agcgccccgg
      601 aactggtaga aaaactgggc gtgaaaagaa ccgtcattaa tcaggccgcc ggaaaaatgg
      661 tacgggaagg g
//
LOCUS       DAZFLD010000688          671 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.691, whole genome shotgun sequence.
ACCESSION   DAZFLD010000688 DAZFLD010000000
VERSION     DAZFLD010000688.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..671
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.691"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..309
                     /locus_tag="ACHKBL_005408"
     CDS             <1..309
                     /locus_tag="ACHKBL_005408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5822100.1"
                     /translation="PQPGDELEITVVSPDRTTTAIITDILNGRPDSTNRSWLVAVRPR
                     RPPGEPEPLTPAGKIMRQYHEEQAHKHNAELTRTLVTINRKAAIARYVHSAAGRQSRR
                     "
     gene            358..528
                     /locus_tag="ACHKBL_005409"
     CDS             358..528
                     /locus_tag="ACHKBL_005409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5822101.1"
                     /translation="MNDTRRISDRELTKMIESARQLLSRQNPVVEETWWENLLAALTE
                     LRERRNNEGAAK"
BASE COUNT          211 a          200 c          173 g           87 t
ORIGIN      
        1 ccacaaccgg gcgatgagct ggaaatcacg gtcgtcagtc ccgatcgcac caccacggcc
       61 atcatcaccg acatactcaa cggacgaccg gacagcacca accgcagctg gcttgtcgcc
      121 gttcgtccgc gccgtccgcc cggagagccg gagccgctca cgcctgccgg aaaaatcatg
      181 cggcagtacc acgaagagca ggcacacaaa cacaacgcag aactcaccag aacactggtc
      241 acaattaacc gcaaagcagc aattgcccgc tatgtgcatt cagccgctgg cagacaatca
      301 cgcaggtaaa caaagaccat gaacataacc agaaacagcc aactaaagga gcgcaccatg
      361 aacgacacca ggaggatcag cgacagagag cttacgaaaa tgattgagtc ggcccggcaa
      421 ctgctttcac gacaaaaccc ggtcgtggaa gaaacgtggt gggagaattt acttgcagca
      481 ctgaccgaac tccgggaacg ccggaacaac gaaggagccg ccaaatgaag accccaggaa
      541 tgaccggaaa ggaaatcatt ttacgctacc tggaaacgca cacacgtttc agcgccccag
      601 aactggtaga aaaactgggc gtgaaaagaa ccgtcattaa tcaggccgcc gggaaaatgg
      661 tacgggaagg c
//
LOCUS       DAZFLD010000689          671 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.692, whole genome shotgun sequence.
ACCESSION   DAZFLD010000689 DAZFLD010000000
VERSION     DAZFLD010000689.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..671
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.692"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(89..547)
                     /gene="tnpA"
                     /locus_tag="ACHKBL_005410"
     CDS             complement(89..547)
                     /gene="tnpA"
                     /locus_tag="ACHKBL_005410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element IS200C family
                     transposase"
                     /protein_id="HFO5822102.1"
                     /translation="MGNEKSLAHTRWNCKYHIVFAPKYRRQVFYREKRRAIGSILRKL
                     CEWKSVRILEAECCADHIHMLVEIPPKMSVSGFMGYLKGKSSLMLYEQFGDLKFKYRN
                     REFWCRGYYVDTVGKNTAKIQDYIKHQLEEDKMGEQLSIPYPGSPFTGRK"
BASE COUNT          157 a          187 c          125 g          202 t
ORIGIN      
        1 ccggaggttt ttcaaatgcg cctataaggc tctcttacca gccgcgccct aacaggcgca
       61 cacgatctga catttgcatc caacttcgtt acttacggcc cgtaaacggg ctgcccggat
      121 agggaatcga taactgctct cccattttat cctcttcaag ctggtgcttt atgtaatcct
      181 gtatcttcgc cgtgttctta cccaccgtat cgacgtagta ccctctgcac cagaactctc
      241 tgttcctgta tttgaatttc aaatcaccaa actgctcgta aagcatcaga ctgcttttcc
      301 ctttcagata tcccataaag ccggatacac tcattttggg cgggatctcc acaagcatat
      361 ggatatgatc tgcacagcat tcagcttcaa gaatccgtac gcttttccac tcacacagct
      421 ttctcaaaat actgcctatt gctctacgct tctctctgta gaacacttgt cttcggtatt
      481 ttggcgcaaa aactatgtga tatttacagt tccatcgggt gtgcgctaag ctcttttcgt
      541 tccccattgg gacccccttt tgatttcttg tttgacactt gcagttgcca gaccgcaagg
      601 tgttttaaca aatcaaaagg ggttttaata actggctcaa agctgaaagc tttccggaac
      661 ccccagccta g
//
LOCUS       DAZFLD010000690          670 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.693, whole genome shotgun sequence.
ACCESSION   DAZFLD010000690 DAZFLD010000000
VERSION     DAZFLD010000690.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..670
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.693"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(341..>670)
                     /locus_tag="ACHKBL_005411"
     CDS             complement(341..>670)
                     /locus_tag="ACHKBL_005411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001175889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
                     /protein_id="HFO5822103.1"
                     /translation="ALWEQALDDVARGKISLEAFMEKQHQWIRHLVLSGRNQTVNLSV
                     ESGPSCPLCNGPTVKRKGKSGFFWGCSRYPDCRGIAGEQGNSRGRKRKINFSHKQKSG
                     DVGDKLN"
BASE COUNT          159 a          197 c          155 g          159 t
ORIGIN      
        1 ccgaagcgac atgtagccac ccgaaggcgc tcctctcagg ccacttgtgg agcattggtg
       61 gtcgccggag cgacgttatg tgcccggaca gactgaaatc actgatggaa aaagtcaaca
      121 gctaaccaaa aacaaaaaag caccggcaag ccggtgcttc agggggatac caaatcccgg
      181 tgttgattct catcttctcc ggagagagcg actcccggca cggttttcac ggagtgcatg
      241 tgccttgtaa ccccgccgtg tgcataacgg gttaccggac ctctcagggc tgtcagcccc
      301 actgtggtca acagggtgat accagaactc gcccgcaatt ttaattcagt ttgtctccga
      361 catcaccaga tttttgttta tgagaaaaat taatttttct tttcctgccc ctgctgtttc
      421 cctgctcacc agcaattccc ctgcagtcgg gataacggga gcatccccag aaaaaaccgc
      481 tttttccctt ccgtttcacc gtcggaccgt tacagagtgg acatgaagga cctgattcca
      541 ctgacagatt aaccgtctgg ttacgtccgg ataacaccag gtgcctgatc cactgatgct
      601 gtttctccat aaacgcctcc agtgagattt tcccccgggc aacatcatcc agcgcctgtt
      661 cccataaagc
//
LOCUS       DAZFLD010000691          670 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.694, whole genome shotgun sequence.
ACCESSION   DAZFLD010000691 DAZFLD010000000
VERSION     DAZFLD010000691.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..670
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.694"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..251)
                     /locus_tag="ACHKBL_005412"
     CDS             complement(<1..251)
                     /locus_tag="ACHKBL_005412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000547696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO5822104.1"
                     /translation="MHIKNFRQYTPENPDVPGAMYLKSEDGQDWYECQSLFSAETLKV
                     VYNSAGVITGISRVASVLWPVGQSVVEVADTEENRKADIS"
     gene            complement(293..>670)
                     /locus_tag="ACHKBL_005413"
     CDS             complement(293..>670)
                     /locus_tag="ACHKBL_005413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000910149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO5822105.1"
                     /translation="PAGPRGAQGPKGEKGDPGPRGERGETGPMGPPGASGDDGKSRVM
                     GFRLGNPVTYELQEGDHGWSVNLADGSVITGLSGNTQGGVSRVTTIYYRPLQMTFDGS
                     HWRTVGVGEYVSSGAPGREYFSL"
BASE COUNT          140 a          234 c          142 g          154 t
ORIGIN      
        1 gagatgtcag ctttgcggtt ttcttccgta tccgccacct ccaccacact ctggcccact
       61 ggccacagaa ctgatgccac cctgctgatg ccggtaatga ccccggcact gttataaacc
      121 accttcagcg tctctgctga aaataacgac tgacactcat accagtcctg accatcctct
      181 gatttcaggt acatcgcacc cggcacatcc ggattttccg gcgtgtattg tctgaaattc
      241 tttatgtgca tctgtaagtc ctcccggtta catgtctgaa agatgatgcg ctttataatg
      301 aaaaatactc acgccccggc gctcctgacg acacatactc accaacacca acggtgcgcc
      361 agtgagagcc atcaaacgtc atctgcagcg gcctgtagta aatcgttgta acacggctga
      421 cgccgccctg agtgttcccc gaaagccctg taatgactga accatcagcc agattaactg
      481 accagccatg gtccccctcc tgcaactcat acgttaccgg attccccagc ctgaagccca
      541 tgacacgact cttcccgtca tctcctgatg cccccggcgg ccccatcggc ccggtttctc
      601 cacgctcacc tctgggaccg gggtcgcctt tctccccttt cggtccctgc gctccccgcg
      661 gaccagcggg
//
LOCUS       DAZFLD010000692          666 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.695, whole genome shotgun sequence.
ACCESSION   DAZFLD010000692 DAZFLD010000000
VERSION     DAZFLD010000692.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..666
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.695"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..551
                     /gene="ydeO"
                     /locus_tag="ACHKBL_005414"
     CDS             <1..551
                     /gene="ydeO"
                     /locus_tag="ACHKBL_005414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acid stress response transcriptional regulator
                     YdeO"
                     /protein_id="HFO5822106.1"
                     /translation="VPVPAKFLRASNVPTGLLNEMIAYLNSEERNHHNFSELLLFSCL
                     SIFATCKGFITLLTNGVLSVSGKVRNIVNMKLAHPWKLKDICDCLYISESLLKKKLKQ
                     EQTTFSQILLDARMQHAKNLIRVEGSVNKIAEQCGYASTSYFIYAFRKHFGNSPKRVS
                     KEYRCQRHTGMNTDNTMSALAI"
BASE COUNT          201 a          128 c          139 g          198 t
ORIGIN      
        1 cggtgcctgt tcctgcgaag tttttacgtg cttcaaatgt accgactgga ttgcttaatg
       61 aaatgattgc ttatctcaac tcggaagaac gcaatcatca taatttttca gaacttttgc
      121 ttttttcttg cctgtctatt tttgccacat gcaaaggttt cattacacta ttaactaacg
      181 gtgtgctatc cgtttccggg aaagtgagaa atattgtcaa catgaagctg gcgcacccat
      241 ggaagctgaa agatatttgt gactgcctgt acattagtga aagcctgttg aagaaaaaac
      301 taaagcaaga gcaaacgaca ttctcacaga ttcttttaga tgccagaatg cagcacgcaa
      361 aaaatttgat acgcgtagaa ggttcagtca ataaaattgc cgaacaatgt ggttatgcga
      421 gtacatctta ttttatttat gcgttccgca aacatttcgg caacagtccg aagagagttt
      481 ctaaggagta ccgttgtcaa aggcacacgg gtatgaacac ggataacacg atgagtgctt
      541 tagctatttg attatttgct aacgagtagt caaccacgca cgttacgtag aaattaaaat
      601 ggggcagcca ttccctgccc cgcgttgttt ttaggcgata tattattgaa ataaataagt
      661 gacatt
//
LOCUS       DAZFLD010000693          661 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.696, whole genome shotgun sequence.
ACCESSION   DAZFLD010000693 DAZFLD010000000
VERSION     DAZFLD010000693.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 661)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..661
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.696"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>661
                     /locus_tag="ACHKBL_005415"
     CDS             <1..>661
                     /locus_tag="ACHKBL_005415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO5822107.1"
                     /translation="SAVLLASGMLMSTAVTAAPGDAAQFGGADTDWSTVDYPRLTDMD
                     DNVDSMGGKIRFTGRVVKATCKVATDSKQIEVVLPVVPSNLFTGIDIEAQGASNQTDF
                     NINLTECSNTEDQKIEFRFTGTADSANKTLANEVEGSTDADNSGNAGATGVGIRIYSK
                     GTTNNGLINLNTTAAEGSASTAAYTIPGNATTHDFSAAFTAGYAQNGSTVAPGVVKST
                     A"
BASE COUNT          183 a          154 c          172 g          152 t
ORIGIN      
        1 tatccgcagt attactggca tcaggtatgc tgatgtctac tgcggtaacc gcagcacccg
       61 gcgatgcagc acaatttggt ggagcggata ctgactggag caccgttgat tatcccaggc
      121 tcactgatat ggatgacaac gttgattcaa tgggggggaa aatccgcttt actggccgtg
      181 tagtgaaagc tacctgtaag gtcgcaaccg attcaaaaca gattgaagtt gtcctgccgg
      241 ttgtgccttc caaccttttc actggtatcg acatagaagc acagggggcg agcaaccaga
      301 ccgatttcaa tattaatctg accgaatgta gcaatacaga ggatcagaaa attgagttcc
      361 gctttaccgg taccgcagat agcgctaata aaacgctcgc taacgaagta gaaggatcaa
      421 cggatgctga caacagcggc aatgcggggg cgactggtgt agggattcga atttactcca
      481 aaggtacgac gaataatggt ctgattaacc tgaatactac tgcggcagag ggtagcgcct
      541 ccaccgccgc ttatacaatt ccaggaaatg ctacgaccca tgatttcagc gcggccttta
      601 ctgcaggtta tgctcaaaac ggtagcactg ttgcacctgg tgtagttaag tcaacagcaa
      661 g
//
LOCUS       DAZFLD010000694          660 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.697, whole genome shotgun sequence.
ACCESSION   DAZFLD010000694 DAZFLD010000000
VERSION     DAZFLD010000694.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..660
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.697"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..183
                     /locus_tag="ACHKBL_005416"
     CDS             <1..183
                     /locus_tag="ACHKBL_005416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000150682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5822108.1"
                     /translation="KEQLIRDEINKEHLTAHQNRINVAEVADSDKSEFSRIKTSSDFD
                     GALWTGIRKLISFFKK"
BASE COUNT          189 a          126 c          160 g          185 t
ORIGIN      
        1 aaagaacagc taattagaga tgaaattaac aaagaacatt tgacagctca tcaaaataga
       61 ataaatgttg ctgaggttgc tgattctgac aagagcgaat ttagtagaat taaaacctct
      121 tcggattttg atggggcatt atggactggg ataagaaagc ttatttcatt ctttaaaaaa
      181 tagatctata gcgtatcgtt ccggcgaact catcctgatg cgttttcggg gggccatcgg
      241 tgaaacatac gaatgatcgc cctctgacga tggatgattt attttgttgc acagaagcga
      301 tcgctgggca ggtaccggag gttggctctg aagctctaat tgcgtcaggt tgcgaagtgt
      361 tgacacgggt attggtgaga actgccccgg gaacgactct gggctgattc tggttctgat
      421 tcgctgtcag cccgtgtggt tactggcctg gctgatttcc tgtcgtcagg cgtttaactc
      481 ccgactgcag gtatcactta ccaagagaag gtaaatcctt ttagacgtac agtgaaagtt
      541 gtctggtcat aatctgaagc atctgacaaa gttgtgtcca ccaaataata agtagtggga
      601 accaaagtgt catatataca gaaaaatata actcaccgat ttccttcacg ttaatacgca
//
LOCUS       DAZFLD010000695          651 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.698, whole genome shotgun sequence.
ACCESSION   DAZFLD010000695 DAZFLD010000000
VERSION     DAZFLD010000695.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..651
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.698"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            256..>651
                     /locus_tag="ACHKBL_005417"
     CDS             256..>651
                     /locus_tag="ACHKBL_005417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5822109.1"
                     /translation="MSPDIISSAVNRFSGWPSNDPLKTDKESMTSPMTVQVIKMIAAI
                     LDPHRIVVGIDTLYTTQRETFTSESQQLIESALQTLHVMNLNNLETNLDEEGHYLAFG
                     YNVDTPDRITVSAYNEKKPGCYGQCLLIQD"
BASE COUNT          199 a          128 c          128 g          196 t
ORIGIN      
        1 ataacatatt tttatcaaag ttaattgatt gttgcgttta tgagcaccgg ccgtaagccc
       61 attatgttat tgtttcatta gtaataatca cttaaatgta tcgatgaaac aggtatgact
      121 tagcgggagt gattgtttct gagtgaacag agaccacttt cgcctgataa atgaaaaatg
      181 agcgattttt gcaagttagt ttttctatag ccgagtacgc tcagcgcctc gcttgattac
      241 aatgaggttg ctgctatgag tcctgatatt atcagttctg ctgtcaatcg cttctcaggt
      301 tggccatcta atgatcctct aaaaacagat aaagaatcaa tgacatcacc catgactgtg
      361 caggtaataa agatgattgc cgccatcctt gacccacatc ggatagtcgt tggaatcgat
      421 acactttata caacgcagcg cgaaacattt acctcagaga gtcaacagct cattgaatca
      481 gcattgcaaa cacttcatgt gatgaattta aataacctgg aaacaaatct cgatgaggaa
      541 ggtcactatc tggcttttgg atataacgtt gacactcctg acagaataac cgtttctgct
      601 tataatgaaa aaaagcctgg ctgctatggc caatgtcttt taatacagga t
//
LOCUS       DAZFLD010000696          649 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.699, whole genome shotgun sequence.
ACCESSION   DAZFLD010000696 DAZFLD010000000
VERSION     DAZFLD010000696.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..649
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.699"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            1..645
                     /locus_tag="ACHKBL_005418"
                     /pseudo
     CDS             1..645
                     /locus_tag="ACHKBL_005418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418332.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="YiiG family protein"
BASE COUNT          221 a          121 c          157 g          150 t
ORIGIN      
        1 gttatattga tgctgccgta gcgttgggta ataccattaa cgaaatggat aaatattaca
       61 tccaggaaaa ttataaagac gatgcgtttg cgaaagggaa aacgctgcac cagacattct
      121 taaaaaatct ggaagccttt gaacctgtag cagaatctta tcatgcggcg attcaggaaa
      181 ttaatgacaa gcgccagctt acggaattga aaaatattga agaaagagaa gggaaaacat
      241 tccactacta ctctctggca gtcatgattt cagcgaaaca aattaataac ctgatatcgc
      301 aagataagtt tgatgcagaa gcggcaatga agaaagtgtc tgaactggaa acgctggtgg
      361 cgcaggccaa agaagcggat aaaagcggca tgaatttctc gtttattaat tcggcaggcc
      421 agtatcaact cgaggctaaa aaatacgttc gccgcatcag agataaagtc ccgtactctg
      481 actgggataa agagcaactt caggacgcaa actcaagctg gatggccgaa gactcatttc
      541 ccgagagcat tatgcgagta caacgaaatg gtcgatgaaa tatttcagtc aattgtaatc
      601 gcggagcggg tttgtgatga gcgcggatat gtcacgaagt cgtgacaat
//
LOCUS       DAZFLD010000697          647 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.700, whole genome shotgun sequence.
ACCESSION   DAZFLD010000697 DAZFLD010000000
VERSION     DAZFLD010000697.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..647
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.700"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..229
                     /locus_tag="ACHKBL_005419"
     CDS             <1..229
                     /locus_tag="ACHKBL_005419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000107923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5822110.1"
                     /translation="NLSNKKSPIYQIKQIYIKEDTKAQLMEFIKGINDECQLLVKPTY
                     IVHCLLNCALQSEGIRKEVINYVANAREKMR"
     gene            229..>647
                     /locus_tag="ACHKBL_005420"
     CDS             229..>647
                     /locus_tag="ACHKBL_005420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000025932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HFO5822111.1"
                     /translation="MSILTTGAFLALAAQCAPNVHPDTSLDVVRVESGLHQYAIAEII
                     PKNERVAEGKSFISHYPESKDEAVKIIRRIEERKRNYSVGLMQINSSNFKKLRVTATE
                     LLEPCSNLKAYQYIITDCYLRGGSLTNALSCYYSGDF"
BASE COUNT          227 a           95 c          138 g          187 t
ORIGIN      
        1 aaacttatca aataaaaaat cgccgatata tcagataaaa cagatatata ttaaagaaga
       61 tacaaaagcg cagttaatgg aatttattaa aggaataaat gatgagtgtc aattacttgt
      121 aaagccaaca tatattgttc attgtctttt aaactgcgca ttacagagcg aaggaatcag
      181 gaaagaagtt attaattatg ttgctaatgc acgagaaaaa atgcgctgat gagtatactg
      241 actaccgggg catttctcgc tctggctgca cagtgtgccc caaacgttca tccagataca
      301 tctcttgatg tggttcgtgt tgagtcgggg ttacatcaat atgctatagc ggaaattata
      361 cctaaaaatg aaagggttgc agagggtaaa agctttataa gtcattaccc ggagagtaaa
      421 gatgaagcag tgaaaataat caggaggatt gaggaacgga aacgaaatta ttctgttgga
      481 ttaatgcaga ttaacagttc aaatttcaaa aaacttagag tgacagctac tgaattgttg
      541 gagccgtgct ctaatctgaa agcgtatcaa tacattatta ccgattgtta tttgcgagga
      601 ggtagcctta ctaacgcgtt aagttgttat tattctggag attttat
//
LOCUS       DAZFLD010000698          646 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.701, whole genome shotgun sequence.
ACCESSION   DAZFLD010000698 DAZFLD010000000
VERSION     DAZFLD010000698.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..646
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.701"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..247
                     /locus_tag="ACHKBL_005421"
     CDS             <1..247
                     /locus_tag="ACHKBL_005421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="conjugal transfer protein TraV"
                     /protein_id="HFO5822112.1"
                     /translation="LTAGDWPVMETDTPTLKRVKRRTFTLIDNRNRQGWITRRFQCFP
                     LAPLPESHSHRVPDILYPLKTEKKECKDNQQPHCLHS"
     gene            208..>646
                     /gene="traW"
                     /locus_tag="ACHKBL_005422"
     CDS             208..>646
                     /gene="traW"
                     /locus_tag="ACHKBL_005422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001189538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraW"
                     /protein_id="HFO5822113.1"
                     /translation="MQRQPATTLLALLIGCAVIPSTHAYSVTVVASQPVTTQIMPELA
                     AIQGVMAEMVRMQTATGTAINQSSEKLASVIAQDGQATRQQMIFSNETRRLEEARKSF
                     SVPDSICSESASGVAAESRRAAASAAARLSQGGGVSSKPIRERL"
BASE COUNT          156 a          201 c          176 g          113 t
ORIGIN      
        1 actgacggcg ggagactggc cggtaatgga aaccgatacc ccgacgctga aacgcgtgaa
       61 gcgtcgtacc ttcacgctca tcgataaccg caaccgtcag ggatggatca cccgtcgctt
      121 ccagtgcttc ccgctggctc cgctgccgga atcacacagt caccgcgtac cagacatcct
      181 gtatcccctg aaaacggaga aaaaagaatg caaagacaac cagcaaccac actgcttgca
      241 ctcctgatag gctgtgcggt tatcccgtcc acccacgcct attctgtgac cgtagtggcc
      301 agccagcctg tcaccacaca aatcatgccg gagctggccg ccattcaggg cgtgatggca
      361 gagatggtcc gtatgcagac agccaccggc acagccatca accagagttc agagaaactg
      421 gcctctgtca ttgcgcagga tgggcaggcc acccgtcagc agatgatatt cagtaatgaa
      481 acccgccggc ttgaggaagc ccggaagagc ttcagcgtgc cggactccat ctgcagtgaa
      541 tccgcctccg gagttgcagc tgaaagtcgc cgtgcagcag cgtctgcggc tgcacgtctg
      601 agtcagggag gaggcgtcag cagtaaacca atccgtgaac gtctct
//
LOCUS       DAZFLD010000699          646 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.702, whole genome shotgun sequence.
ACCESSION   DAZFLD010000699 DAZFLD010000000
VERSION     DAZFLD010000699.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..646
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.702"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..353)
                     /gene="tssK"
                     /locus_tag="ACHKBL_005423"
     CDS             complement(<1..353)
                     /gene="tssK"
                     /locus_tag="ACHKBL_005423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004171008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
                     /protein_id="HFO5822114.1"
                     /translation="MKIYRPLWSEGVLLSPQQFQQQTEWESFRSAGISALASPFPWGV
                     EKVELNDSLLSSGLIQITQLRLWLEDGTLIDAQRSDLPPAPRELDAGQLADHDAVTVV
                     IALPHMQPGVINVEQ"
     gene            complement(369..>646)
                     /locus_tag="ACHKBL_005424"
     CDS             complement(369..>646)
                     /locus_tag="ACHKBL_005424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012104626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
                     /protein_id="HFO5822115.1"
                     /translation="MIQRENIGTTKDRRLLELELNNWIRGLVTEMTDPGDELQASHPL
                     RDGKVVVEDIEDNPGFFRVKLYAVPHFQVEGMDVSLSLVSQMPKAKA"
BASE COUNT          155 a          197 c          151 g          143 t
ORIGIN      
        1 tcctgctcaa cattaatcac gccaggctgc atgtggggaa gtgcaataac gacagttacc
       61 gcatcgtggt cagccagctg accggcatct aactctcgcg gtgccggagg caaatcgctc
      121 ctttgagcat caataagtgt tccgtcttcc agccagaggc gtaactgcgt aatctggatc
      181 agcccggatg acagaagact gtcattaagc tcaacctttt ccactcccca gggaaacggg
      241 gatgccagcg cagaaatacc ggcactgcga aaagactccc attcagtctg ctgctgaaac
      301 tgctgagggg agagcaaaac accttcgctc cacagcggac ggtaaatctt catttcccga
      361 cgcctgcctt acgcttttgc cttcggcatc tgcgaaacca gggacagact gacatccata
      421 ccttccacct ggaaatgcgg tacagcgtag agtttcacac ggaaaaatcc cgggttgtct
      481 tcgatatctt ccaccaccac ttttccgtca cgcagcggat gcgaggcctg caactcatca
      541 cccggatcgg tcatctccgt caccagaccc cggatccagt tattcagctc cagctcaagc
      601 agacggcgat ctttggtggt accgatattt tcccgctgaa tcatct
//
LOCUS       DAZFLD010000700          642 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.703, whole genome shotgun sequence.
ACCESSION   DAZFLD010000700 DAZFLD010000000
VERSION     DAZFLD010000700.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..642
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.703"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(105..>642)
                     /locus_tag="ACHKBL_005425"
     CDS             complement(105..>642)
                     /locus_tag="ACHKBL_005425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001305022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFO5822116.1"
                     /translation="ISAMLYIRKNADKWHLQRHRIGVLGFSAGGHLASTFVNQICDFK
                     RSSDSKEQEIFAIPDFVGLIYPVISMKDDVTHPDSRKQLLGDNSTSENIIQYSADLNV
                     TSCFPPVFLLHCCDDDLVSIENSLLMYRGLIEKGVSAEMHLYEKGGHGFSVQWATDLP
                     VGGWIKLFMDWMYSHGFK"
BASE COUNT          215 a          140 c          109 g          178 t
ORIGIN      
        1 gagcatgatt gtgataaagc tgaacagcta atgtataatc acctttataa acgaattgaa
       61 gcatcatagt tactcgacgc ttcaccaggt agtcaccata aatattattt aaatccatga
      121 gaatacatcc agtccataaa cagttttatc catcctccaa caggaagatc tgtggcccac
      181 tgaacactaa agccatgccc tcctttctca tagagatgca tttcagcaga aacgcctttc
      241 tctattaggc cacggtacat gagcagactg ttttcaattg acacaagatc atcatcacaa
      301 caatggagta aaaacacagg aggaaaacat gatgtgacat taagatccgc actatattgt
      361 attatatttt ctgaggtact attgtccccc agtaattgct tcctggagtc tggatgtgtc
      421 acatcatctt tcattgatat tacaggataa attaacccga caaaatcggg aatagcgaaa
      481 atctcttgtt ctttactgtc agaagaacgt ttgaaatcac aaatctgatt cacaaacgtt
      541 gaggcgagat gccccccagc agaaaatcct aatacaccaa tacgatgacg ctgtagatgc
      601 catttatcag cattcttacg aatataaagc attgcagata tt
//
LOCUS       DAZFLD010000701          641 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.704, whole genome shotgun sequence.
ACCESSION   DAZFLD010000701 DAZFLD010000000
VERSION     DAZFLD010000701.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..641
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.704"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..249
                     /locus_tag="ACHKBL_005426"
     CDS             <1..249
                     /locus_tag="ACHKBL_005426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tox-REase-5 domain-containing protein"
                     /protein_id="HFO5822117.1"
                     /translation="KDKLCQFWEAKARYDQFFRADGSKKPWWTGNLSAINQASRHQAV
                     ATVNQPLKVVWIFMQPVSCEYFRKMFKDYKDIITRWQP"
     gene            237..>641
                     /locus_tag="ACHKBL_005427"
     CDS             237..>641
                     /locus_tag="ACHKBL_005427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015386758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5822118.1"
                     /translation="MATVIELFNTSINIHSLKERDITIDDCLNELYRSTVVVKNLTQH
                     DHIWYLTGYSRKDASKHVVFSDNEPNQNTIDTFERHYKKNRPLINESIWNGQPDGLAC
                     SISHFMKFIDNPKKLNLIIDIDQRVANTSDMID"
BASE COUNT          226 a          105 c          129 g          181 t
ORIGIN      
        1 aaagataagc tgtgtcagtt ctgggaagct aaagcgcggt atgaccaatt tttcagggct
       61 gatggttcaa agaaaccctg gtggacaggt aatctaagcg caattaatca agcatcccga
      121 catcaggcgg ttgcaacagt caatcagcca ttgaaagttg tctggatatt tatgcaacct
      181 gtaagttgtg agtatttcag gaaaatgttt aaagattaca aggatattat tacgaggtgg
      241 caaccgtgat tgaattattt aacacgtcaa taaatataca ttctttgaaa gaaagagaca
      301 taacgataga cgattgtttg aatgaactat acagaagtac cgtagttgtt aaaaatctaa
      361 cgcagcacga tcacatatgg tacttaactg gctactcaag aaaggacgct tcaaagcatg
      421 tcgtgtttag tgataatgaa ccgaaccaaa atacaataga tacctttgag cggcattata
      481 aaaagaatcg tcctttaatt aatgaatcta tatggaatgg acaacccgat gggctggctt
      541 gctcaatatc acattttatg aaatttattg ataaccctaa aaaactaaat cttatcatag
      601 atattgatca gagagttgcc aatacttcgg atatgattga t
//
LOCUS       DAZFLD010000702          640 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.705, whole genome shotgun sequence.
ACCESSION   DAZFLD010000702 DAZFLD010000000
VERSION     DAZFLD010000702.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..640
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.705"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>640)
                     /locus_tag="ACHKBL_005428"
     CDS             complement(<1..>640)
                     /locus_tag="ACHKBL_005428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2345 domain-containing protein"
                     /protein_id="HFO5822119.1"
                     /translation="LENAAERAQALKADIDSQIQMFEQRLKLLNEAVLFSAPEGMALT
                     SGEDMQMSAARNVAVNAGGDISTGTMGNLTALAGEKLGLFARTGQLSLKSGEGAVDVQ
                     AQNGSMRLFAEKKLTISSESDISFTGKKRITLIGGGSYLRLEAGKVEYGTMATCMRRV
                     KRTMTEAAQSMPMTFPRMSSSHIYSAIYRLLDKHGNILANTPCRLTTPSGQTV"
BASE COUNT          131 a          201 c          151 g          157 t
ORIGIN      
        1 ttactgtctg accagatgga gtggtgaggc gacagggggt attggccaga atattgccgt
       61 gtttgtccag caaccgataa atagccgagt agatatgcga agaactcata cgggggaatg
      121 tcatcggcat actctgcgca gcctccgtca tcgtccgctt cacccgacgc atacaggttg
      181 ccatcgtacc atactcgacc ttcccggctt caagccgcag gtaactcccc ccgccaatca
      241 gcgtgatgcg tttcttgccg gtaaacgaga tatcactttc tgacgaaatg gtcagcttct
      301 tctcagcaaa caaccgcatg ctgccgtttt gcgcctgaac atccactgcc ccttccccgg
      361 atttcaggct aagttgaccg gtccgggcaa aaagccccag cttctctccg gccagcgcgg
      421 tcaggttgcc cattgttccg gtactgatat cccccccggc gttgacagcc acatttcgtg
      481 ctgcactcat ctgcatatct tcaccgctgg tcagcgccat tccttccggt gccgagaaaa
      541 gcactgcttc attgagcagt ttcagccgct gctcaaacat ctgtatctga ctgtcaatat
      601 cagccttcag cgcctgtgcc cgttctgcgg cattttccag
//
LOCUS       DAZFLD010000703          639 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.706, whole genome shotgun sequence.
ACCESSION   DAZFLD010000703 DAZFLD010000000
VERSION     DAZFLD010000703.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..639
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.706"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..636)
                     /locus_tag="ACHKBL_005429"
     CDS             complement(<1..636)
                     /locus_tag="ACHKBL_005429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000376547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO5822120.1"
                     /translation="MDSCIQKKQVWYKHLSPRQWKIFGAAWTGYLLDGFDFVLISLVL
                     TEVQHEFGLTTIEAASLISAAFISRWFGGLAIGALSDKMGRRMAMVLSIVLFSLGTLA
                     CGLAPGYAVMFIARIVIGLGMAGEYGSSVTYVIESWPVHLRNKASGFLISGFSIGGGL
                     AAQVYSIVVPLWGWRSLFFVGMLPILFAFYLRKNLPESDDWQKRQQENKPVR"
BASE COUNT          213 a          164 c          116 g          146 t
ORIGIN      
        1 tctgacgggt ttattctctt gttgcctttt ttgccagtca tcagactccg gaagattctt
       61 gcgaagataa aaagcaaata atattggaag catgccgaca aagaaaagag aacgccagcc
      121 ccatagtggt actacaatgc tataaacctg tgcagctaaa cctcccccaa tagaaaatcc
      181 agaaattaaa aaaccacttg ccttgtttct gagatgaaca ggccagcttt cgataacata
      241 tgttacactt gagccatatt cacctgccat accaagccca ataacaatgc gagcaataaa
      301 catcactgca tatccaggag ccagaccaca ggcaagggtt ccgagagaga atagcactat
      361 acttaaaacc attgccatcc tgcgccccat cttgtccgat aaagcgccaa tagcaagccc
      421 cccaaaccat cgggaaataa aagcagcgga aataaggctt gccgcttcta ttgtcgtcaa
      481 gccgaattca tgctgaacct ctgtaagaac cagagatatc agaacgaaat caaaaccatc
      541 aagtaaataa cctgtccatg ccgcaccgaa tatcttccac tgtctgggtg aaagatgctt
      601 ataccaaacc tgctttttct gaatacagga gtccatatt
//
LOCUS       DAZFLD010000704          638 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.707, whole genome shotgun sequence.
ACCESSION   DAZFLD010000704 DAZFLD010000000
VERSION     DAZFLD010000704.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..638
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.707"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(10..>638)
                     /locus_tag="ACHKBL_005430"
     CDS             complement(10..>638)
                     /locus_tag="ACHKBL_005430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFO5822121.1"
                     /translation="PKENLYGMHVYSRMILSDAEIQYLVDKEIPSMHMIVHMPEGQRI
                     RLHCLHPVPPSPTESDESTDRDSELLMVGKSVADSELPVILAGDLNDVAWSATTRLFM
                     KISGLLDPRRGRGFFSTFHSHYFFLRWPLDHVFHSQHFLLKSLRRLPDMGSDHFPVMA
                     ELVYAPAHGMQQESLEKEKSYEVLAQKKMDMTDSTAEDVHTPGGQKKS"
BASE COUNT          179 a          154 c          120 g          185 t
ORIGIN      
        1 atatttacgt caggattttt tctgcccgcc aggagtatga acatcttccg ccgttgagtc
       61 tgtcatatcc atttttttct gtgccagtac ttcatatgat ttttcttttt ccagactctc
      121 ctgctgcatc ccgtgagccg gagcatatac aagctcagcc atcactggaa aatgatctga
      181 acccatatcc ggtaaacggc gtaaactttt aagcagaaaa tgctggctgt gaaatacatg
      241 gtcaagcggc catcgcagga aaaaataatg gctgtgaaac gtgctaaaaa aaccacgtcc
      301 ccggcgtgga tccagcagtc cacttatttt catgaataaa cgagtggtcg cagaccacgc
      361 aacatcattc agatcccccg ctaatattac aggtaattca gaatctgcaa cacttttccc
      421 gaccattaaa agttctgagt cacgatcagt tgactcatca ctttcagtcg ggctcggtgg
      481 aacaggatga agacaatgta atcttattct ctgaccttcc ggcatatgaa ctatcatatg
      541 catggaggga atttccttgt ccactaaata ctgtatttct gcatcactta aaatcatcct
      601 tgaatataca tgcatgccat ataaattttc tttcgggc
//
LOCUS       DAZFLD010000705          635 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.708, whole genome shotgun sequence.
ACCESSION   DAZFLD010000705 DAZFLD010000000
VERSION     DAZFLD010000705.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..635
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.708"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>635
                     /locus_tag="ACHKBL_005431"
     CDS             <1..>635
                     /locus_tag="ACHKBL_005431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFO5822122.1"
                     /translation="WDGDHLTTVQTDTTRIQTVYEPGSFTPLIRIETDNGEREKTQRR
                     SLAEKLQQEGSEDGHGVVFPAELVRMLDRLEGEIRADRVSSESRQWLAQCGLTVERLA
                     AQIEPVYLPERKIHLYHCDHRGLPLALISTEGATEWRGEYDEWGNLLNEENPHHLHQP
                     YRLPGQQHDEESGLYYNRNRYYDPLQGRYITQDPIGLEGGWNLYNYPLNPI"
BASE COUNT          163 a          161 c          213 g           98 t
ORIGIN      
        1 tgggacggcg accacctgac gacggtgcag accgacacca cgcgtatcca gacggtatac
       61 gagccgggaa gcttcacacc gctcatccgc atcgaaacag acaacggcga gcgggagaaa
      121 acgcagcggc ggagcctggc ggagaagctg cagcaggaag gaagcgagga cggccacggt
      181 gtggtgttcc cggcagaact tgtgaggatg ctggacaggc tggaggggga aatccgggca
      241 gaccgcgtga gcagtgaaag ccgccagtgg ctggcacagt gcgggctgac ggtggagcgg
      301 ctggcggcac agatagagcc ggtttattta cctgagcgaa aaattcatct ttatcactgt
      361 gaccaccggg ggctgccgct ggcgctcatc agtaccgaag gagcaacgga atggcgtggg
      421 gaatatgatg aatggggcaa cctgctgaat gaggagaacc cgcatcacct gcaccagcca
      481 taccgtctgc cggggcagca gcatgatgag gaatcaggac tgtactataa ccggaaccgg
      541 tactatgacc cgctacaggg gaggtatatt acacaagacc ccattgggct ggagggggga
      601 tggaacctgt ataattaccc actgaatccg ataat
//
LOCUS       DAZFLD010000706          634 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.709, whole genome shotgun sequence.
ACCESSION   DAZFLD010000706 DAZFLD010000000
VERSION     DAZFLD010000706.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..634
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.709"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>634
                     /locus_tag="ACHKBL_005432"
     CDS             <1..>634
                     /locus_tag="ACHKBL_005432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015974229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III restriction-modification system
                     endonuclease"
                     /protein_id="HFO5822123.1"
                     /translation="SKDNSDKDDKIEQEINEILHDKELLLSLENPRRFIFSKWTLREG
                     WDNPNVFQICKLRSSGSTTSKLQEVGRGLRLPVNEYMCRVKNRDFTLNYYVDFTEKDF
                     VDSLVKEVNDSSFKETVPGKFTQELKDKILSQYPELSSRTLMNEIFDAEIIDDNENFK
                     DSDAYSRLKAKYPAAFPAGVKPSKIKKATDGKKRTKMRIGKFSELKELWDL"
BASE COUNT          212 a          106 c          131 g          185 t
ORIGIN      
        1 tcaaaagata atagtgataa agatgataaa attgagcagg aaatcaacga aatccttcat
       61 gataaagagc ttctattgtc tttagaaaat cctcgccgtt tcattttctc taaatggacg
      121 ttgcgtgagg gatgggataa cccaaatgtc tttcagattt gtaagctccg ttcgagcggt
      181 agcaccacat ctaaacttca ggaggtaggg cgtggtttac gtcttccagt gaatgaatac
      241 atgtgccgtg tgaaaaaccg cgattttact ctcaactatt atgttgattt tacggaaaaa
      301 gattttgttg attctcttgt aaaagaggtt aatgatagtt ctttcaaaga aactgttcca
      361 ggtaagttta ctcaagaact taaggataaa atcttatctc agtatcctga gctttcatcc
      421 agaacactca tgaatgagat ctttgatgct gaaattattg atgataatga aaacttcaaa
      481 gattccgatg cctatagccg tttaaaagca aaatatccgg ctgcattccc tgccggggtg
      541 aaaccaagca aaataaaaaa agccacggat ggaaaaaagc gtactaaaat gcgtattggt
      601 aagttcagtg agttgaaaga actatgggat ttga
//
LOCUS       DAZFLD010000707          633 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.710, whole genome shotgun sequence.
ACCESSION   DAZFLD010000707 DAZFLD010000000
VERSION     DAZFLD010000707.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..633
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.710"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(125..>633)
                     /gene="fimA"
                     /locus_tag="ACHKBL_005433"
     CDS             complement(125..>633)
                     /gene="fimA"
                     /locus_tag="ACHKBL_005433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HFO5822124.1"
                     /translation="AVSAAEGDESVTTTVNGGVIHFKGEVVNAACAIDSESMNQTVEL
                     GQVRSSRLAKAGDLSSAVGFNIKLNDCDTNVSSNAAVAFLGTTVTSNDDTLALQSSAA
                     GSAQNVGIQILDRTGEVLILDGATFSAKTDLIDGTNILPFQARYIALGQSVAGTANAD
                     ATFKVQYL"
BASE COUNT          184 a          160 c          129 g          160 t
ORIGIN      
        1 ccaaaacaca aactattcct cattaataat gagatgaaca ttggtgaatt tctcgggtgt
       61 gcgtagccat gccacacaca ccctgtttca atttcagatg acgtatagcc atcttaagat
      121 tgaattatag atattgaact ttgaaggtcg catcagcgtt tgcagtacca gctactgact
      181 gcccgagagc aatataacga gcctggaacg gcaatatatt cgtgccatca atcaagtcag
      241 ttttagcgct aaaagtggca ccatcaagta ttaatacctc ccccgtacgg tccaaaattt
      301 gaataccgac attttgggca gagcctgccg ctgaactctg cagcgctaac gtatcgtcat
      361 tactggtgac agtagtaccc aggaatgcaa cagctgcatt actggaaaca ttggtatcac
      421 aatcattcag cttgatattg aagccaacgg cggagctgag gtcacccgct ttagccagac
      481 gagaagaacg gacctgaccc agctcaaccg tttggttcat tgattctgaa tcaatcgcac
      541 aagcggcatt taccacttca cctttaaaat gaataacacc gccattaaca gtggtcgtta
      601 ctgattcatc accctcggct gcgcttaccg cag
//
LOCUS       DAZFLD010000708          633 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.711, whole genome shotgun sequence.
ACCESSION   DAZFLD010000708 DAZFLD010000000
VERSION     DAZFLD010000708.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..633
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.711"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..328)
                     /locus_tag="ACHKBL_005434"
     CDS             complement(<1..328)
                     /locus_tag="ACHKBL_005434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001433618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraG N-terminal
                     domain-containing protein"
                     /protein_id="HFO5822125.1"
                     /translation="MTTNNYLEYFLTLLGWVVNNGLWNVLISTGLFALPLAFRIIGIW
                     LKVREEGADEGNKGMLSLPRIEHVLYGAFFVMISCCVPLVQVTLDTLSFDKSRAQTCG
                     VWTPEEP"
     gene            complement(335..628)
                     /locus_tag="ACHKBL_005435"
     CDS             complement(335..628)
                     /locus_tag="ACHKBL_005435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000906498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5822126.1"
                     /translation="MYTLLLTGITVFSLWLADTGIRHAEGFAALREWMYETRYGWLGW
                     RLCLYSAIGWLGWRIWHAPGFRAEYRASFLRMATVSVLFILLCEYAIFAEPGR"
BASE COUNT          176 a          199 c          136 g          122 t
ORIGIN      
        1 cgggctcttc aggtgtccat accccacagg tctgtgccct ggatttatcg aatgacagcg
       61 tatccagcgt cacctgtacc agaggaacac agcaggatat catcacaaaa aaagcaccat
      121 agagaacatg ctcaatacgc ggcagggaaa gcatcccttt gtttccctca tccgcacctt
      181 cttccctgac tttcagccag atcccgatga tcctgaatgc cagtggcagg gcaaacagtc
      241 ctgtgctgat caacacattc cacagaccgt tattcaccac ccagcccagc agagtcagaa
      301 aatattccag atagttattt gtggtcatga ttgctcaacg ccccggctca gcaaaaatgg
      361 cgtattcaca cagcaggata aacagaacac tgactgtggc catacgcaga aaagaagccc
      421 ggtattctgc cctgaacccc ggcgcatgcc agatcctcca ccccagccat ccgatcgcac
      481 tgtaaagaca cagacgccat cccagccagc cataacgtgt ctcatacatc cactcccgca
      541 gggccgcaaa tccttctgcg tgacgtatac cggtatcggc cagccagagg gaaaacacgg
      601 taatccctgt cagcaacagc gtatacaggg cga
//
LOCUS       DAZFLD010000709          627 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.712, whole genome shotgun sequence.
ACCESSION   DAZFLD010000709 DAZFLD010000000
VERSION     DAZFLD010000709.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..627
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.712"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            1..627
                     /locus_tag="ACHKBL_005436"
                     /pseudo
     CDS             1..627
                     /locus_tag="ACHKBL_005436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001289064.1"
                     /note="internal stop; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
BASE COUNT          183 a          137 c          174 g          133 t
ORIGIN      
        1 ccgtcggtgg ccccgcagtg ggtgacgcgg acgaatcatg cggtgagcga cctgacgggc
       61 cgtccgctga tgctctttaa cagtgaaggt aaaaccgtgt ggcgaccgtg acaaaccagc
      121 ctgtgggggc tggcactcag tctgcccgca gacaccggct acccggaccc gcgcggggag
      181 ctggacccgg aagccgaccc cggcctgctg tatgcaggac agtggcagga tacggaatcc
      241 gggctgtgct ataaccggtt ccggtattac gagccggaaa cagggatgta cctggtgagt
      301 gacccgatag ggctgagagg cgggataaac aattatgcat atgttcataa tccccttggt
      361 tatattgatc cattaggatt aaccatttgc gatatcgcaa aaacaaggga tcttgcaaca
      421 acaataagtg ataagtttaa acagcttttt caatgcgata aatttgccgc agcgttaaaa
      481 agtaaaatgc aacaagctgg ggtaaatggt gagcatctca tactcgaaac taaaaataaa
      541 caaggtgcct ttggcaatat atggagtgat agaataggta aaaatatagc aactaatggt
      601 catcatgaag caataaaagt aggtgat
//
LOCUS       DAZFLD010000710          624 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.713, whole genome shotgun sequence.
ACCESSION   DAZFLD010000710 DAZFLD010000000
VERSION     DAZFLD010000710.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..624
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.713"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..581)
                     /locus_tag="ACHKBL_005437"
     CDS             complement(<1..581)
                     /locus_tag="ACHKBL_005437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold-containig protein"
                     /protein_id="HFO5822127.1"
                     /translation="MILFADYNTPYLFAISFVLLIGLLEIFALICGHMLSGALDAHLD
                     HYDSITTGHISQALHYLNIGRLPALVVLCLLAGFFGLIGILLQHTCLMVWQSPLSNLF
                     VVPVSLLFTIIAVHYTGKIVAPWIPRDHSSAITEEEYIGSMALITGHQATSGNPCEGK
                     LTDQFGQIHYLLLEPEEGKIFTKGDKVLIICRLS"
BASE COUNT          203 a          120 c          144 g          157 t
ORIGIN      
        1 gaaagtcggc aaataattaa cactttgtct cctttagtga aaatttttcc ttcttcaggc
       61 tccagtagta aataatgtat ttggccaaat tgatcggtga gttttccttc gcagggatta
      121 cctgaagtgg cttgatgacc ggtaattaac gccatgctac caatatattc ttcttctgta
      181 atagctgagc tgtgatcgcg tggaatccag ggcgcaacta ttttaccggt ataatgcacc
      241 gcaataatcg taaacagtaa actgacagga accacaaaca ggttcgagag tggtgattgc
      301 cagaccatga ggcaggtatg ctgcaacagg atgccaataa gaccaaaaaa accagccagt
      361 aagcagagaa cgacgagagc tggcaatctt ccgatattga gataatgaag tgcctggcta
      421 atatgacctg tggttatgga atcataatgg tcaagatgtg catcaagagc accggagagc
      481 atgtgaccgc agataagcgc aaaaatttct aaaaggccga tcaacagtac aaaggaaata
      541 gcgaaaagat aaggggtgtt atagtcggcg aataaaatca tatgtcactc cgatgtccat
      601 ttagtgacgc aatatttact ttag
//
LOCUS       DAZFLD010000711          624 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.714, whole genome shotgun sequence.
ACCESSION   DAZFLD010000711 DAZFLD010000000
VERSION     DAZFLD010000711.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..624
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.714"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..602
                     /locus_tag="ACHKBL_005438"
     CDS             <1..602
                     /locus_tag="ACHKBL_005438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type VI secretion IcmF C-terminal
                     domain-containing protein"
                     /protein_id="HFO5822128.1"
                     /translation="DSGRITTFLKTHLGGLVHQEGNHWVAEPSASQGMVVSPDFLRAI
                     NQLAELSDIVFSQGDAGVHFELMARPSRDVARVQLTLDEQNLDYFNQMESWQSFTWPG
                     NTYYPGVSLSWRSVSSGMQLYASHQGNWGFIRLLDEALITPLDASRTQLVWITADGNP
                     LKFVMRSELGDGPLALLKLQGFTLPTAIFNVAAGTEGVE"
BASE COUNT          130 a          163 c          187 g          144 t
ORIGIN      
        1 gcgactcggg tcggatcacg acgttcctga aaactcatct tggcgggctc gtccaccagg
       61 aggggaatca ctgggtcgct gagccttccg caagccaggg gatggttgtc agtccggatt
      121 tcctccgggc gattaatcag ctggcagagc tgtcagatat cgtgttctct cagggcgatg
      181 cgggggttca cttcgaactg atggcgcgtc cttcacggga tgttgccaga gtgcagctta
      241 cccttgatga acagaatctg gattacttca atcagatgga aagctggcag agcttcacct
      301 ggccggggaa tacttactat ccaggtgtgt cgctcagctg gcggagtgta agcagtggta
      361 tgcaactcta cgccagtcac caggggaact gggggtttat tcgcctgctg gacgaagccc
      421 tgatcacgcc acttgatgcc agccgcacac aactggtgtg gatcactgcc gatggtaatc
      481 ctctgaaatt cgtcatgcgc agcgagctgg gcgacggtcc gcttgcctta ctgaagctac
      541 aggggtttac gctgcccacc gccattttta acgttgccgc cggcacggaa ggtgttgagt
      601 aaaaatgaac aggggcattt atcc
//
LOCUS       DAZFLD010000712          624 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.715, whole genome shotgun sequence.
ACCESSION   DAZFLD010000712 DAZFLD010000000
VERSION     DAZFLD010000712.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..624
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.715"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>624
                     /locus_tag="ACHKBL_005439"
     CDS             <1..>624
                     /locus_tag="ACHKBL_005439"
                     /EC_number="2.7.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000629094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HFO5822129.1"
                     /translation="AVDIGGTKISAALISDDGSFLLKKQISTPHERCPDEMTGALRLL
                     VSEMKGTAERFAVASTGIINNGVLTALNPDNLGGLKEYPLKNIMEDITGLNGSVINDA
                     QAAAWAEYTVLPKEICDMVFITVSTGVGGGIVVNRKLLTGVSGLAGHVGHILSGVTDT
                     ECGCGRRGCVEAVSSGRAIMGAAKNELAGYSTKYIFELARQGYKEAE"
BASE COUNT          195 a           89 c          172 g          168 t
ORIGIN      
        1 tggctgttga tataggagga acgaaaattt cagcggcgtt gatatctgat gacggttcat
       61 ttttgctgaa aaaacagata tcaacacctc atgaaagatg tcctgacgaa atgacagggg
      121 ctttacgtct actggtttct gaaatgaagg gaacagcgga gcgatttgca gtagcatcaa
      181 cagggattat taataatgga gtattgacag ctttgaatcc agacaatctt ggagggctga
      241 aagagtatcc tctgaaaaat ataatggaag atataactgg tttaaatgga agtgtgataa
      301 atgatgccca ggcagccgca tgggcagaat atacagtttt gccaaaagaa atttgtgata
      361 tggtttttat aaccgtttct acgggggttg gagggggaat tgtagttaac agaaaattat
      421 taacaggtgt ttctggatta gccggacatg ttgggcatat attgtccgga gtaacggata
      481 cagaatgtgg atgtggtcgt cggggatgcg ttgaagcggt ttcatctggt cgggccatca
      541 tgggggcagc aaaaaatgaa cttgcaggat atagcaccaa atatattttt gaattagcga
      601 gacagggata taaggaagcc gaat
//
LOCUS       DAZFLD010000713          623 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.716, whole genome shotgun sequence.
ACCESSION   DAZFLD010000713 DAZFLD010000000
VERSION     DAZFLD010000713.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..623
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.716"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..357
                     /locus_tag="ACHKBL_005440"
     CDS             <1..357
                     /locus_tag="ACHKBL_005440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001534332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO5822130.1"
                     /translation="HTHGGSVSNTDLGTKTTSSFDYGTKTTSTNGQHEHSVPLRRQDG
                     GGINFDWLDGSSSGSHIGNGYVPAAGDHQHTVPIGAHTHTLALGSHGHGLTINPTGNA
                     ENTVKNIAFNYIVRLA"
     gene            357..>623
                     /locus_tag="ACHKBL_005441"
     CDS             357..>623
                     /locus_tag="ACHKBL_005441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFO5822131.1"
                     /translation="MTFKMSEHPQTIKIYNLSAGTNEFIGEGDAWIPPHTGLPAHSTD
                     IAPPDIPEGHVAIFDEDENTWQLMEDHRGKTVWRLESGSALLITE"
BASE COUNT          179 a          176 c          142 g          126 t
ORIGIN      
        1 cacacccacg gggggagtgt ctcaaatact gaccttggca cgaaaacgac ctcatcattt
       61 gactacggaa ccaaaacaac ctctacaaac ggacaacatg aacacagcgt ccccctgaga
      121 agacaagatg gcggaggcat aaactttgac tggctggatg gttcatcgtc cggttctcat
      181 attggtaatg gatacgttcc tgccgcaggt gaccaccaac ataccgtacc cattggcgca
      241 cacacccaca cgctcgcact gggttcacat ggtcacggcc tgaccattaa ccccaccggt
      301 aatgccgaaa acaccgttaa aaatattgca tttaactaca tcgtgaggct tgcataatga
      361 cctttaaaat gagcgaacac ccacagacaa ttaaaatcta caacctgtca gccggaacaa
      421 atgaatttat cggcgaaggc gatgcctgga tccctccgca taccggactg cctgcgcact
      481 caacggacat tgccccgcct gacattcctg aaggacacgt ggctatcttt gatgaggatg
      541 aaaacacctg gcagctcatg gaagatcacc ggggtaaaac cgtctggcgt ctggagtcgg
      601 gaagcgcctt actgattact gag
//
LOCUS       DAZFLD010000714          616 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.717, whole genome shotgun sequence.
ACCESSION   DAZFLD010000714 DAZFLD010000000
VERSION     DAZFLD010000714.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..616
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.717"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>616
                     /locus_tag="ACHKBL_005442"
     CDS             <1..>616
                     /locus_tag="ACHKBL_005442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="autotransporter adhesin Ag43"
                     /protein_id="HFO5822132.1"
                     /translation="GTTTLNNGATLTLSGKTVNNDTLTIHEGDALLQGGALTGNGRVE
                     KSGSGTLTVSNTTLIQKTVNLNEGTLTLNDSTVTTDVIAQRGTALKLTGSTVLNGAID
                     PTNVTLTSGATWNIPDNATVQSVVDDLSHAGQIHFTSARTGKFVPTTLQVKNLNGQNG
                     TISLRVRPDMAQNNADRLVIDGGRATGKTILNLVNAGNSGTGLAT"
BASE COUNT          160 a          188 c          168 g          100 t
ORIGIN      
        1 gggcaccacc acgctgaata acggtgccac gcttaccctt tccgggaaaa cggtgaataa
       61 cgataccctg accatccatg aaggtgatgc actcctgcag ggaggcgctc ttaccggtaa
      121 cggcagggtg gaaaaatcag gaagtggcac actcactgtc agcaacacca cactcatcca
      181 gaaaaccgtc aacctgaatg aaggcacgct gacgctgaac gacagtaccg tcaccacgga
      241 tgtcatcgct cagcgcggca cggccctgaa gctgaccggc agcacagtgc tgaacggtgc
      301 cattgacccc acgaatgtca ccctgacatc cggtgccacc tggaatatcc ccgataacgc
      361 cacggttcag tcggtggtgg atgacctcag ccatgccgga cagattcatt tcacctccgc
      421 ccgcacaggg aagttcgttc cgacaaccct gcaggtgaaa aacctgaacg ggcagaatgg
      481 caccatcagc ctgcgtgtac gcccggatat ggcgcagaac aatgctgaca gactggtcat
      541 tgacggtggc agggcaaccg gaaaaaccat cctgaatctg gtgaacgccg gcaacagtgg
      601 tacggggctg gcgacc
//
LOCUS       DAZFLD010000715          612 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.718, whole genome shotgun sequence.
ACCESSION   DAZFLD010000715 DAZFLD010000000
VERSION     DAZFLD010000715.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..612
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.718"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>612
                     /locus_tag="ACHKBL_005443"
     CDS             <1..>612
                     /locus_tag="ACHKBL_005443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF927 domain-containing protein"
                     /protein_id="HFO5822133.1"
                     /translation="LVIELPVKKKTSEQSEDELKPRVECRPDGVYWVTPKVDKQSGEI
                     IRPCEWIGDRMRTVGIGHDGCDGYLIIELEPEGMEKIIYEAMPRNEIGLPSGWSRLRG
                     RGVAVTTSQKLLNKLAEYLQREGERTQWEVTSTAGWHCGAYVMPDGEIIGVPERPVAF
                     CGGSAAIKGYVVRGTADEWRDNVASLMRGNHSMMLGVLVGLAAP"
BASE COUNT          146 a          129 c          193 g          144 t
ORIGIN      
        1 ctggttattg agctgcctgt aaagaaaaaa acatcagaac aatcagagga tgaattaaaa
       61 ccccgcgttg aatgccgtcc tgatggcgtt tactgggtta cgccaaaggt tgataagcaa
      121 tccggtgaaa ttatccgccc gtgtgagtgg attggtgacc gtatgcgaac cgtggggatc
      181 ggccatgatg gctgcgatgg gtatctgata atcgaacttg aaccagaggg aatggaaaaa
      241 attatttatg aggccatgcc ccgcaatgaa atcggcctcc cttctggatg gtcgcgattg
      301 cgtgggcgcg gggtggcagt gacaaccagt cagaaacttt taaacaaact tgcggaatac
      361 cttcagcgtg aaggagagcg gacacagtgg gaggtcacat caacggcggg gtggcattgt
      421 ggcgcttatg tcatgccgga tggtgagatt attggcgtac cagaaaggcc cgttgccttc
      481 tgtggtggtt cggcggcgat taaaggttat gtggttcggg gtacggctga tgagtggcgc
      541 gacaatgttg cctcactgat gcgcggtaat cactcaatga tgcttggagt gctggtcggg
      601 cttgccgccc cc
//
LOCUS       DAZFLD010000716          612 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.719, whole genome shotgun sequence.
ACCESSION   DAZFLD010000716 DAZFLD010000000
VERSION     DAZFLD010000716.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..612
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.719"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..131)
                     /locus_tag="ACHKBL_005444"
     CDS             complement(<1..131)
                     /locus_tag="ACHKBL_005444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AcaB family transcriptional regulator"
                     /protein_id="HFO5822134.1"
                     /translation="MPQEKTGEVRKSEQVGALQSSLTIALHTRYAIRLWEGRRNNQK"
     gene            177..>612
                     /locus_tag="ACHKBL_005445"
     CDS             177..>612
                     /locus_tag="ACHKBL_005445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5822135.1"
                     /translation="MRITDNKKTNQAEYTFNADYFLIAVDTGNTAHFLTSVATGSTVR
                     FLTSAATGSTVHFLTSAATGSTVRFLTSAATGSTVRFLTSAATGSTAHFLISAATGST
                     VHFLTSSATGSTVRFLTSAATGSTVHFLTSAATGSTAHFLIPA"
BASE COUNT          123 a          177 c          115 g          197 t
ORIGIN      
        1 tccttctgat tattcctgcg tccctcccag agacggatcg catatcgggt atgaagggca
       61 atagtcagcg atgactgcag agcaccaact tgttctgatt ttctgacttc tccggttttc
      121 tcctggggca ttgtgtttac tccaaatgat ttcatgacca gaagatagtc cgcaggttgc
      181 gtattaccga caacaaaaaa acaaatcagg cagaatatac atttaacgcc gattattttt
      241 taatcgctgt tgacactggc aacaccgctc actttttaac ctctgttgcc actggcagca
      301 ctgttcgctt tttaacctct gctgccactg gcagcactgt tcacttttta acctctgctg
      361 ccactggcag cactgttcgc tttttaacct ctgctgccac tggcagcact gttcgctttt
      421 taacctctgc tgccactggc agcactgctc attttttaat ctctgctgcc actggcagca
      481 ctgttcactt tttaacctct tctgccactg gcagcactgt tcgcttttta acctctgctg
      541 ccactggcag cactgttcac tttttgacct ctgctgccac tggcagcact gctcattttt
      601 taatccctgc tg
//
LOCUS       DAZFLD010000717          611 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.720, whole genome shotgun sequence.
ACCESSION   DAZFLD010000717 DAZFLD010000000
VERSION     DAZFLD010000717.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..611
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.720"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..526
                     /locus_tag="ACHKBL_005446"
     CDS             <1..526
                     /locus_tag="ACHKBL_005446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5822136.1"
                     /translation="GLFQRFSKLLPFRIDFAYRKDTPSFGHSCKHSMCMEIYRLLSET
                     QTMLAGYYWVMEYTPDKGLHIHFIGYLDGQRHKNSYQISRQLGDIWRRITEGEGYFHL
                     CRAKDKYPVRIDHVIHYSDKSAVDDLRYALSYLAKQDQKEHGIILRRSRLPEKSNRGR
                     PRLDSTLSEICSLV"
BASE COUNT          163 a          136 c          154 g          158 t
ORIGIN      
        1 aggtctgttt caacgttttt ctaaactgtt acctttcaga attgattttg cctacaggaa
       61 agacacgccc agttttggtc attcctgtaa gcacagtatg tgtatggaaa tataccgttt
      121 acttagcgaa acccaaacga tgctggcggg atattactgg gtaatggaat atacaccaga
      181 caaagggctt catattcatt ttatcggcta tctggatggg caacgtcaca agaactctta
      241 tcagatatcc aggcaactgg gggatatctg gaggcggatc acggaagggg agggttattt
      301 tcatctgtgc cgggctaaag acaaataccc ggtgcgtatc gatcacgtga ttcactatag
      361 tgataaatct gcggtagacg acttacgtta cgccctcagc tacctggcta aacaggatca
      421 gaaagaacac ggtattattc tgaggcgtag caggcttccc gaaaaaagta accggggccg
      481 accgcgactg gacagtaccc tgtcggagat atgttctctg gtgtaagccc ctgaacgaca
      541 actgcgggct ggcgctaatt gttacagtcg gttccggagg ccatttcagc gacactgcgc
      601 atttttacga t
//
LOCUS       DAZFLD010000718          609 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.721, whole genome shotgun sequence.
ACCESSION   DAZFLD010000718 DAZFLD010000000
VERSION     DAZFLD010000718.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..609
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.721"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            315..>609
                     /gene="gadE"
                     /locus_tag="ACHKBL_005447"
     CDS             315..>609
                     /gene="gadE"
                     /locus_tag="ACHKBL_005447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HFO5822137.1"
                     /translation="MIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIKKVGNNPFKVI
                     IDTYHNHILDEEAIKFLEKLDAERIIVLAPYHISKLKAKAPIYFVSRKESIKNL"
BASE COUNT          215 a           96 c          111 g          187 t
ORIGIN      
        1 tacattttaa ctttagtaat attcttcaga gatcacaaac tggttattga taacttattc
       61 ttgggcagta atccgcaaaa gttaaccttt tgtttactag ttacaaactg ataacaacca
      121 ggaatcttac ttaggaccaa tatatggagt gcgtgatgga taaatctgaa gtattgatta
      181 gtgttaatag acgtattagt tcacgaaggg caaagttctt ataggcgttt actatattga
      241 acaacgattc ggacaaggat gtaaataatg aaaaggatga catgttcgaa acgataacgg
      301 ctaaggagca agttatgatt tttctcatga cgaaagattc ttttctttta cagggctttt
      361 ggcagttgaa agataatcac gaaatgataa aaatcaattc cctgtcagag atcaaaaaag
      421 taggcaataa ccccttcaag gttatcattg atacctatca caatcatatc cttgatgaag
      481 aagcgattaa atttctggag aaattagatg ccgagagaat tattgttttg gcaccttatc
      541 acatcagtaa actaaaagct aaagcgccta tttattttgt cagccgcaaa gaaagtatca
      601 aaaatcttc
//
LOCUS       DAZFLD010000719          607 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.722, whole genome shotgun sequence.
ACCESSION   DAZFLD010000719 DAZFLD010000000
VERSION     DAZFLD010000719.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..607
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.722"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            17..607
                     /locus_tag="ACHKBL_005448"
     CDS             17..607
                     /locus_tag="ACHKBL_005448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFO5822138.1"
                     /translation="MSRIYAYCRVSTLEKTTKNQRREIESAGFNIKSQQIIEEQISGS
                     AATSERPGFNQLLDRLKRGDALIVTKLDRLGCNPMDISKTVEQLTKAGIKVHCLALGG
                     VDLTSPPGKMMMQVISAVAEFERDLLLERTHSGILRARGAGKRFGRPPVLNDEQIQRV
                     LEQIKSGINISDIAQEFKTSRQTILRAKAKNQTPDI"
BASE COUNT          208 a          117 c          137 g          145 t
ORIGIN      
        1 tatagaagat ctcactatgt ctcgaattta tgcttactgt cgggtatcaa ctctggaaaa
       61 gacaaccaaa aatcaacgtc gggaaatcga aagtgcaggt tttaacatca aatctcagca
      121 aataattgaa gaacaaatta gcggctcagc agcaaccagt gagcgtcctg gttttaacca
      181 gttgcttgat cgactgaaaa gaggtgatgc attgattgtc acaaaactgg atcgcctcgg
      241 ttgtaatcca atggatatca gtaaaacggt ggaacaactg accaaagcag gtatcaaagt
      301 gcattgctta gcattggggg gcgttgacct gaccagtcca cctggaaaaa tgatgatgca
      361 agtaatttca gcagtcgctg aatttgagcg agacctttta cttgagcgta ctcattcagg
      421 gatattaaga gcacggggcg caggaaagcg atttggtcgc ccacctgtgt taaatgatga
      481 acaaatacag agggtactag agcaaattaa gtcaggtata aatataagtg acattgccca
      541 agaattcaaa acctcgcggc aaaccatttt aagagctaaa gcaaaaaatc agacacctga
      601 tatataa
//
LOCUS       DAZFLD010000720          601 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.723, whole genome shotgun sequence.
ACCESSION   DAZFLD010000720 DAZFLD010000000
VERSION     DAZFLD010000720.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..601
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.723"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            388..549
                     /locus_tag="ACHKBL_005449"
     CDS             388..549
                     /locus_tag="ACHKBL_005449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5822139.1"
                     /translation="MTDRSPTEVIHGIIEEQEERELILMIEEQRERQRKQESTQPDSV
                     SAENQADVT"
BASE COUNT          207 a          136 c          150 g          108 t
ORIGIN      
        1 gaagcctctt cgatggggtg aaaaatccct gaaaaaatgc gcaaaaaact gcgcaaaaaa
       61 gtgcgcaaat cgatcataaa accgatcaca aaatcgatca cacagacaaa cagagcaaag
      121 ccagatatga cgcggcttgc agggggatag accacctcaa aaagtgcgca aaaaaatgcg
      181 caaaacgaaa cgcaacagcc gcgcaaaacg aaaccgatac gtgatgatcg atttttacgc
      241 cagaaatgac cagagcaagg gggctgattt ccacccttgt attttgtgac ttacttaaag
      301 taacccgcaa aaatgaacgt ttttacaggg cagaaacgag agtactggtt aacattcctg
      361 gcatcaccgc aactcacagg gggacctatg accgaccgtt caccaacaga ggtaattcac
      421 gggattattg aagagcagga ggagcgcgag ttaatcctca tgattgaaga gcagagagaa
      481 cgccagcgca aacaggagtc cacgcagccg gacagcgtga gcgctgaaaa tcaggctgat
      541 gtgacgtgat ggtgtcgaac tgagtagctt taagcgactc tgtgaccagt tttaaacgag
      601 t
//
LOCUS       DAZFLD010000721          600 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.724, whole genome shotgun sequence.
ACCESSION   DAZFLD010000721 DAZFLD010000000
VERSION     DAZFLD010000721.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..600
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.724"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>600
                     /locus_tag="ACHKBL_005450"
     CDS             <1..>600
                     /locus_tag="ACHKBL_005450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001367806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hemagglutinin"
                     /protein_id="HFO5822140.1"
                     /translation="LKDDKGGSATINGGKDNKNGNTLTVKGDGDIAVTVNDKGMTIDG
                     TTLKNEIKNDAIKIKGGDGKTLTKSLGDNVEITGKGPIKTQVAGDKLEISAETASLPV
                     NGGNGKITVNNGDGDKLVTAKNIAEAINQSGWKIKADKDTGGVIEGNGQGTETLIKPG
                     DMVTLKAGKNLSVKQSGGEFTFSVIDTPSFSSVQLNGNG"
BASE COUNT          213 a           87 c          160 g          140 t
ORIGIN      
        1 gctaaaggat gataagggag gttctgcgac tattaatggt gggaaggata ataagaatgg
       61 taacacttta actgttaaag gtgatggaga tattgcagtt actgttaatg ataaaggaat
      121 gacgattgac ggaacaacgc tgaaaaatga aataaaaaat gatgccatca aaattaaagg
      181 tggagatggt aagacgctga cgaagagtct tggtgacaat gtcgaaatca ctggcaaagg
      241 cccaataaaa acccaggtgg ctggagataa actggagatt tcagcagaga cagcttcgtt
      301 gcctgtaaat ggtggtaacg ggaagattac agtaaacaat ggtgatggcg ataaacttgt
      361 tactgcgaaa aacattgctg aagccataaa ccagtctggc tggaaaatta aagcggataa
      421 agataccggt ggtgttatcg agggaaatgg tcagggaaca gaaacactga taaaacccgg
      481 tgatatggtt acgctgaaag ccggtaaaaa tctcagcgtg aaacaaagtg gtggtgaatt
      541 tacattctct gttattgata ctccgtcatt cagcagtgtt cagctgaacg gtaatggaaa
//
LOCUS       DAZFLD010000722          599 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.725, whole genome shotgun sequence.
ACCESSION   DAZFLD010000722 DAZFLD010000000
VERSION     DAZFLD010000722.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..599
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.725"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..228
                     /locus_tag="ACHKBL_005451"
     CDS             <1..228
                     /locus_tag="ACHKBL_005451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001720563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RAQPRD family integrative conjugative element
                     protein"
                     /protein_id="HFO5822141.1"
                     /translation="ATVMRQLDHVQAALERARTASLQASSQGERYFFDYLRARADLQT
                     IRDGIDHYLAPSRAQPRSSAAVTGDYRRERP"
     gene            228..470
                     /locus_tag="ACHKBL_005452"
     CDS             228..470
                     /locus_tag="ACHKBL_005452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03758 family integrating conjugative element
                     protein"
                     /protein_id="HFO5822142.1"
                     /translation="MAMTALQNTAFRAASGEVDLSVLHLITLGFLLAVVFLWAAWSLT
                     DAWAGWRSGDVRDGVFGWFMFRLVLLLICSIWMFAG"
BASE COUNT           97 a          157 c          186 g          159 t
ORIGIN      
        1 gccactgtaa tgcgccagct tgaccacgtg caggcagcgc tggagcgggc cagaacggcc
       61 tctctgcagg cttcttcaca gggggaacgc tacttttttg attatctgcg tgcccgtgca
      121 gatctgcaga ccattcgtga cggtattgac cattatctgg ccccttcccg ggcgcaaccc
      181 cggtcttccg cagccgtgac cggtgactac cggcgggagc gtccctgatg gcgatgacgg
      241 ccctgcagaa tacggccttc cgggctgctt caggtgaagt ggatctcagt gtgctgcatc
      301 tcatcacgct gggatttctg ctggcagttg tgttcctctg ggcagcgtgg agtctgacgg
      361 atgcctgggc aggctggcgt agcggtgatg tccgggatgg tgtgttcggc tggtttatgt
      421 tccgactcgt cctgctgctg atctgcagta tctggatgtt tgcgggttga tttcagtgtc
      481 ttgtttaagt ttcaggagga aataatgcat aaaaaaatgc gcatctgtcg ccaggcctgg
      541 tatgccatgg cggtgtttct gtgtaccggg atcacacctg ctctggctga tttgcctca
//
LOCUS       DAZFLD010000723          598 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.726, whole genome shotgun sequence.
ACCESSION   DAZFLD010000723 DAZFLD010000000
VERSION     DAZFLD010000723.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..598
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.726"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..456)
                     /gene="dcuC"
                     /locus_tag="ACHKBL_005453"
     CDS             complement(<1..456)
                     /gene="dcuC"
                     /locus_tag="ACHKBL_005453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DcuC"
                     /protein_id="HFO5822143.1"
                     /translation="MFNVITGCVILIITVVLLIKRYDTRTTLIGAGILLCIVAMSPLN
                     AFSAFASRMTESGLIQAICTSMGFACVMKFTGCDTCLVHFLTKGLSRLGIFLIPAAVT
                     VSFIVCIAIPSAAGASAAVGATLIPLLISARIHPAIAGAAILSGTIGAYL"
BASE COUNT          213 a          117 c          123 g          145 t
ORIGIN      
        1 gaggtaagca ccgatagttc ctgaaaggat agctgctccg gctattgcag gatgaatacg
       61 tgctgaaata agtaatggta tcagcgttgc accaacagca gctgatgctc cagcagcaga
      121 tggaatcgcg atgcaaacaa taaaactgac ggtaaccgca gcaggaataa gaaagatacc
      181 aagacgagat aatcctttcg ttagaaaatg aaccaggcat gtatcacaac cagtgaattt
      241 cataacacag gcaaagccca tactggtaca aatggcctga atgagaccgg actcagtcat
      301 tctgcttgca aatgccgaaa atgcgtttaa aggtgacatg gcaactatac atagcagaat
      361 acctgcacca attagtgtcg ttcgtgtgtc gtatcttttt atcaaaagca caactgtgat
      421 tatcagaatc acacatccag ttatcacgtt aaacatgaga tcctccttct tcaaagaagg
      481 ataatcttta ctttatgtga tttgctcaaa tattgatcat gaacaaaaaa atcaattaac
      541 cggggatact attatgatgg aggtgataac agaaaattat caggcgaaaa aatgaaat
//
LOCUS       DAZFLD010000724          595 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.727, whole genome shotgun sequence.
ACCESSION   DAZFLD010000724 DAZFLD010000000
VERSION     DAZFLD010000724.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..595
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.727"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            14..>595
                     /gene="acrS"
                     /locus_tag="ACHKBL_005454"
     CDS             14..>595
                     /gene="acrS"
                     /locus_tag="ACHKBL_005454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrS"
                     /protein_id="HFO5822144.1"
                     /translation="MAKRTKAEALKTRQELIETAIAQFAQHGVSKTTLNDIADAANVT
                     RGAIYWHFENKTQLFNEMWLQQPSLRELIQDHLTAGLEHDPFQQLREKLIVGLQYIAK
                     IPRQQALLKILYHKCEFNDEMLAEGVIREKMGFNPQTLREVLQACQQQGCVANNLDLD
                     VVMIIIDGAFSGIVQNWLMNMAGYDLYKQAPALV"
BASE COUNT          171 a          126 c          146 g          152 t
ORIGIN      
        1 tgaataatta atcatggcaa aaagaaccaa agccgaagct ctgaagaccc ggcaagaact
       61 gattgaaact gccatcgccc agtttgcgca acatggcgta agcaagacga cgctcaacga
      121 cattgccgac gctgctaacg ttacgcgtgg cgctatctac tggcacttcg agaacaagac
      181 ccaactgttt aatgagatgt ggttgcaaca gccttcattg cgggagttaa tccaggatca
      241 cttgaccgct gggttagagc atgacccgtt tcagcaattg cgtgaaaaat tgattgtcgg
      301 tttgcaatat attgccaaaa ttccccgcca gcaggcgttg ctgaaaatct tatatcacaa
      361 atgtgaattt aatgatgaga tgctggccga gggagtgata cgcgaaaaga tgggctttaa
      421 tccgcagact ctccgcgaag tattgcaggc gtgtcagcaa caaggttgtg tagcaaataa
      481 tctcgattta gatgttgtga tgattattat tgatggtgcc ttcagcggaa ttgttcaaaa
      541 ctggttaatg aatatggcgg gttatgatct ttataaacaa gcccccgctc tggtc
//
LOCUS       DAZFLD010000725          592 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.728, whole genome shotgun sequence.
ACCESSION   DAZFLD010000725 DAZFLD010000000
VERSION     DAZFLD010000725.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..592
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.728"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>592
                     /locus_tag="ACHKBL_005455"
     CDS             <1..>592
                     /locus_tag="ACHKBL_005455"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFO5822145.1"
                     /translation="NQTLEQMFQALFKSWFVDFDPVIDNALDAGNPIPETLQSRAELR
                     QNVRNSTDFKPLPAEIRSLFPSEFEETELGWVPKGWESETFDSFCDLIQSGGTPSRKE
                     TSFWDGGTIKWLSSGEVKGKIVLDTKEKITDTGLLNSSSKLWEKYTTVVAMYGATAGE
                     VCIIGDKMAANQACCGLYSKTFPFFVYNFVCNKANEL"
BASE COUNT          187 a          103 c          138 g          164 t
ORIGIN      
        1 aatcaaaccc tggaacaaat gttccaagcc ctgttcaaat cctggtttgt agattttgat
       61 ccggtaattg ataacgctct ggatgcagga aatccaatcc cggaaaccct gcaatctcgt
      121 gccgagttgc gtcaaaatgt acgtaatagt acagatttca aaccgcttcc ggcggaaatt
      181 cgctcgcttt tcccaagtga atttgaagaa acggagttgg gttgggtgcc aaagggttgg
      241 gaaagtgaaa ctttcgattc attctgcgac ttaattcaga gtgggggaac gccttcaagg
      301 aaggaaactt cattctggga tggcggtaca ataaaatggc tttcatctgg cgaagtgaaa
      361 ggaaaaatag tcttagatac aaaagaaaaa atcacagata ctggattact aaatagttca
      421 tctaaattat gggaaaaata tacaaccgtt gttgcaatgt acggtgcgac tgcgggtgaa
      481 gtgtgtataa ttggtgataa aatggcggcc aatcaagcat gttgtggatt gtattcaaag
      541 acattcccat tttttgtcta taattttgtc tgcaataaag caaatgaatt ag
//
LOCUS       DAZFLD010000726          585 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.729, whole genome shotgun sequence.
ACCESSION   DAZFLD010000726 DAZFLD010000000
VERSION     DAZFLD010000726.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..585
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.729"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>585
                     /locus_tag="ACHKBL_005456"
     CDS             <1..>585
                     /locus_tag="ACHKBL_005456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO5822146.1"
                     /translation="ESLYITPSAVSQSLQRLRAQFNDPLFIRSGKGIAPTTTGLNLHH
                     HLEKNLRGLEQTINIVNKSELKKNFIIYGPQLISCTNNNMLIRCLRQDTSIDIECHDI
                     LLSAENAEELLVQRKTDLAITLQPVISRSVICMPLHTIRNSLICSNKHPRITDTSNYE
                     QIIAEEFTLLISKSAGIDEIQMDIDERFINRKVS"
BASE COUNT          192 a          131 c           95 g          167 t
ORIGIN      
        1 agaatcatta tatataacgc cctctgctgt tagtcagtcg ttacaacgtt tacgtgcgca
       61 atttaatgac ccgttattca ttcgctcagg gaaaggtatc gcgccgacta caacagggtt
      121 aaacctgcat caccatcttg agaaaaatct cagaggcctt gaacaaacga tcaatatcgt
      181 taataagtct gaactaaaga aaaattttat tatctacggt cctcagctta tttcttgtac
      241 taataacaac atgctaatcc gctgtcttag acaagatact tcaatcgata tagaatgcca
      301 tgacatcctg ctttcagctg aaaatgccga agaactgttg gttcaacgca aaactgacct
      361 ggcaattaca ctgcaaccag taattagtcg ctctgtcatc tgcatgccat tacacactat
      421 tcgcaattcc cttatatgta gcaacaaaca tcctcgtatt actgatacca gtaactatga
      481 acaaattatc gccgaagaat tcaccctgtt gatatctaaa tcagcaggga ttgacgaaat
      541 tcaaatggac atagatgaac gttttataaa ccgtaaagtt tcatt
//
LOCUS       DAZFLD010000727          580 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.730, whole genome shotgun sequence.
ACCESSION   DAZFLD010000727 DAZFLD010000000
VERSION     DAZFLD010000727.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 580)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..580
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.730"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>580
                     /locus_tag="ACHKBL_005457"
     CDS             <1..>580
                     /locus_tag="ACHKBL_005457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GPO family capsid scaffolding protein"
                     /protein_id="HFO5822147.1"
                     /translation="DAAETYSRNTYTAMLWPWHEEEIGHRQYTSNLGEVDSLKAETGE
                     DGKTRLYARLVPNQYLIEANRLGQKLFTSAEIVENFARSGRDYLLGVAVTDIPASLGT
                     EKIRFTLNNEEYTAQRANCQTFTLGSLSPDTTHAPSKPESFLKRLFSAGQTDNTDNTD
                     TSKPDTGEDAKMDEIKALLEKLITLVEGGSAAA"
BASE COUNT          160 a          175 c          159 g           86 t
ORIGIN      
        1 tgatgccgct gaaacctatt cccgcaacac ctacacggcg atgctgtggc cctggcatga
       61 ggaagaaatc ggacaccgcc agtacaccag taatctgggt gaagtggact cactgaaagc
      121 ggaaaccggc gaggacggta aaacccgcct ctacgcgcgg ctggtcccta accagtacct
      181 gattgaagcc aacagactgg ggcagaagct gttcacatcc gccgagatag tggagaactt
      241 cgcccggagc ggacgcgact atctcctggg cgtggcggtg acggatatcc ccgccagtct
      301 ggggacggaa aaaatacgct tcaccctgaa caacgaggaa tacacagcac agcgggcgaa
      361 ctgccagacg ttcacgctgg gcagcctgtc accggacacc acccatgccc ccagtaaacc
      421 tgaatcattc ctgaaacgcc tgttcagcgc aggacagacc gacaacaccg acaacaccga
      481 taccagtaag cctgatacag gcgaggacgc aaaaatggac gaaattaaag cactgcttga
      541 aaagctgatc accctggtgg aaggcggcag cgccgctgcc
//
LOCUS       DAZFLD010000728          578 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.731, whole genome shotgun sequence.
ACCESSION   DAZFLD010000728 DAZFLD010000000
VERSION     DAZFLD010000728.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..578
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.731"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            193..525
                     /gene="hdeA"
                     /locus_tag="ACHKBL_005458"
     CDS             193..525
                     /gene="hdeA"
                     /locus_tag="ACHKBL_005458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeA"
                     /protein_id="HFO5822148.1"
                     /translation="MKKVLGVILGGLLLLPVVSNAADAQKAADNKKPVNSWTCEDFLA
                     VDESFQPTAVGFAEALNNKDKPEDAVLDVQGIATVTPAIVQACTQDKKASFKDKVKGE
                     WDKIKKDM"
BASE COUNT          189 a          104 c          120 g          165 t
ORIGIN      
        1 taagaatgca gtcgatttaa taaaaatttc ctaattacag tatctgatgc gtctgtaact
       61 cattgtattg aaataaaaat atctgatttt gatattttcc atcaacatga catatacaga
      121 aaaccaggtt ataacctcag tgtcgaaatt gattcgtgac ggctctttca ctttatagtt
      181 gaggatatta cgatgaaaaa agtattaggc gttattcttg gtggtctgct tcttctgcca
      241 gtagtgagca atgcagcgga tgcacaaaaa gcagctgata acaaaaaacc ggtcaactcc
      301 tggacctgtg aagatttcct ggctgtggac gaatccttcc agccaactgc agttggtttt
      361 gctgaagcac tgaacaacaa agataaacca gaagatgcgg ttttagatgt tcagggtatt
      421 gcaaccgtaa ccccagctat cgttcaggct tgtactcagg ataaaaaagc cagctttaaa
      481 gataaagtta aaggcgaatg ggacaaaatt aagaaagata tgtaattccg ggaatgcgtt
      541 acatcgtatt tccttgcata ttgaacaggc cggaatat
//
LOCUS       DAZFLD010000729          572 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.732, whole genome shotgun sequence.
ACCESSION   DAZFLD010000729 DAZFLD010000000
VERSION     DAZFLD010000729.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..572
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.732"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            83..>572
                     /locus_tag="ACHKBL_005459"
     CDS             83..>572
                     /locus_tag="ACHKBL_005459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000149054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HFO5822149.1"
                     /translation="MTHKPYISPSLSLYHAQAHDVLRMLPENSVDALITDPPYSSGAT
                     HKAGRTCQGSHAKYINDEDLHRFEGFEGENMDARSWEYWTHLWMTQAWRTVRPGGYAL
                     VFTDWRQLPALTDAFQAAGFTWRGIVAWNKGRASRTPHTGYFRHQCEYVVWGSKGALG
                     KSP"
BASE COUNT          130 a          163 c          170 g          109 t
ORIGIN      
        1 ctgggcatta tgggcagcgc ctttattgaa gaatgctttt gccgtctggc agaccgctac
       61 ctgaagcaga aggaacgcag aaatgaccca taagccttac atttcgcctt ccctgagcct
      121 gtatcacgcg caggcgcatg acgttctgcg gatgctgccg gaaaacagtg tggacgcgct
      181 catcacagac ccgccataca gttcaggtgc cacacacaag gcggggcgga catgccaggg
      241 cagccacgcc aaatacatca acgatgaaga tcttcaccgc tttgaaggct tcgaaggcga
      301 gaacatggac gcccgttcat gggaatactg gacgcatctg tggatgacac aggcatggcg
      361 cacggtacga cccggtggtt acgccctggt cttcaccgac tggcggcagt tgcccgcgct
      421 gacggacgca ttccaggccg ccggctttac ctggcggggg atcgtggcat ggaacaaggg
      481 acgggcctca agaacaccgc ataccggtta ttttcgtcac cagtgcgaat atgtggtctg
      541 gggcagtaaa ggcgcgctgg gcaaatcccc tt
//
LOCUS       DAZFLD010000730          572 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.733, whole genome shotgun sequence.
ACCESSION   DAZFLD010000730 DAZFLD010000000
VERSION     DAZFLD010000730.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..572
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.733"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            8..>572
                     /locus_tag="ACHKBL_005460"
     CDS             8..>572
                     /locus_tag="ACHKBL_005460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000834027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5822150.1"
                     /translation="MKSDLTIKNRYCTIPQTKFRKWDEMDVLLWKLGKNDSRRRSGVY
                     YLNAYKDAYVQYNRDKIIKHAYAAGIRPELLGGVAWIESGGMPENYKFQIYETKRMIG
                     SLDMPENKTSFGSMGIQIRTAAITLGLDPSELTTRNQLELATCLMEDDFTFKIAATHL
                     RDLALFDYPSSATLYMTNEQYIMAGIRY"
BASE COUNT          196 a           93 c          117 g          166 t
ORIGIN      
        1 tttaaatatg aaaagcgatc tcactataaa aaatagatat tgtacaattc cacaaacgaa
       61 gttccgtaaa tgggatgaaa tggacgtttt actctggaag ttaggaaaaa atgattcgcg
      121 tcgccgttca ggagtatatt atttaaatgc ttataaagat gcatatgtac aatataatag
      181 ggacaaaata attaaacatg catatgccgc agggattcgt ccagagttac tgggcggcgt
      241 ggcctggatt gagtcaggag gtatgcctga aaattataag tttcagatat atgaaacaaa
      301 aagaatgatc ggatcgttag atatgcctga aaataaaaca tcatttggct caatgggtat
      361 acaaatacga accgcagcga ttactctagg ccttgaccct tcagaattaa ctaccagaaa
      421 tcagttagag ttagcgacat gcctgatgga agatgatttt acctttaaaa ttgctgctac
      481 tcatttaaga gatcttgccc tttttgatta tccctcttcg gcaacactgt atatgacgaa
      541 tgagcagtat attatggctg gtattagata ta
//
LOCUS       DAZFLD010000731          571 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.734, whole genome shotgun sequence.
ACCESSION   DAZFLD010000731 DAZFLD010000000
VERSION     DAZFLD010000731.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..571
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.734"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            86..541
                     /locus_tag="ACHKBL_005461"
     CDS             86..541
                     /locus_tag="ACHKBL_005461"
                     /inference="COORDINATES: protein motif:HMM:NF014417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006354 - DNA-templated transcription
                     elongation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0140673 - transcription elongation-coupled
                     chromatin remodeling [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination NusG
                     family protein"
                     /protein_id="HFO5822151.1"
                     /translation="MTTRKSGFNWYVLEFCSARTARVFSHLERLGVTFWCPLTCSHYR
                     RSDKNCFRRKEVPLFPGYVFIKIDFNKTHSTTVTSVPWVKSFVSFGGEPVPVCEEIIL
                     GLRRRETNYWGEYSPIRGIPHELAEILLENNPMKRSFLFFKYLSNTQNK"
BASE COUNT          162 a           91 c          135 g          183 t
ORIGIN      
        1 attaaaagtt gttttgattc tgcgcaaaaa aacagggcta cggatacttc atgactgatc
       61 ggggatcccc gaggcggagg tgtgcatgac gactaggaaa agcggcttca actggtatgt
      121 tctagagttt tgttctgcac gtacagcacg agtgttttct catctggaaa ggttaggggt
      181 aacattctgg tgtcccctga cttgttccca ttatcgtaga tcagataaaa attgtttcag
      241 acggaaagaa gtgccgctgt ttcctggata tgtgttcata aagatcgatt tcaataagac
      301 ccatagcaca actgttacgt cagttccttg ggtaaagtca tttgtgtcat ttggtggtga
      361 gcctgttcct gtttgcgagg aaatcattct gggattgaga agaagagaga ctaattattg
      421 gggagaatat tctcctatac gcggaatacc tcatgaattg gctgaaatct tgttagaaaa
      481 taatccgatg aaaagaagtt ttttattttt taaatattta agcaatacgc agaataagta
      541 gttgaaaatc ttggtttatt atcttttgtg t
//
LOCUS       DAZFLD010000732          571 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.735, whole genome shotgun sequence.
ACCESSION   DAZFLD010000732 DAZFLD010000000
VERSION     DAZFLD010000732.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..571
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.735"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            216..>571
                     /locus_tag="ACHKBL_005462"
     CDS             216..>571
                     /locus_tag="ACHKBL_005462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5822152.1"
                     /translation="MAGSRASGDVTIIMIAENTHDLFIQSMAALTAVLPVPVFTQAQR
                     MAQAAASLSTDKMQIPITTDTLPAPVHLSVATMRSAVSAARAAQARQLAAQRPDAAGL
                     RRQISRFISRRAGALAG"
BASE COUNT          128 a          188 c          155 g          100 t
ORIGIN      
        1 attgtctggc ctgaatcccc ttcgccggat catcacgcag gctgaccgga ggcaccatga
       61 cgtggacaag aaaaacaatc agcgtcagcg ccgacatgcc tccgctgagc tgttccgtca
      121 tccccgtcac accgtggact tacgggctgg gacgatatga agaatcgggg gtatatttaa
      181 gccctccgaa cgccataaac tggctggccg ggaaaatggc cggaagccgt gcatccggcg
      241 acgtgaccat tatcatgatt gccgaaaaca cccacgatct ttttatacag tcaatggcgg
      301 cactgaccgc cgttctgccc gtgccggtat tcacgcaggc acagcgcatg gcacaggctg
      361 ccgccagtct gagcactgac aaaatgcaaa tcccgatcac caccgacacc ctgcccgcac
      421 ctgtgcatct ttccgttgcc acaatgcgca gcgcagtcag tgccgcccgt gcagcacagg
      481 ccaggcagct ggcagcacaa cgcccggatg ccgcagggct tcgccgccag ataagccgct
      541 ttatcagcag acgggctggc gcacttgccg g
//
LOCUS       DAZFLD010000733          571 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.736, whole genome shotgun sequence.
ACCESSION   DAZFLD010000733 DAZFLD010000000
VERSION     DAZFLD010000733.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..571
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.736"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            149..331
                     /locus_tag="ACHKBL_005463"
     CDS             149..331
                     /locus_tag="ACHKBL_005463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001304601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1378 family protein"
                     /protein_id="HFO5822153.1"
                     /translation="MTFIHQVMLYFCTAVCVLYLLSGGYRAVRDFWRRQIDKRAAEKI
                     SASQSAGSKPEEPVTP"
     gene            357..>571
                     /locus_tag="ACHKBL_005464"
     CDS             357..>571
                     /locus_tag="ACHKBL_005464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF826 domain-containing protein"
                     /protein_id="HFO5822154.1"
                     /translation="MSEITSLVTAEAVKEVLRSEEVLSALKQKLRQNLESRLDAEVDA
                     ILDELLGAPAVPEPEGIAGDGSASDGG"
BASE COUNT          149 a          135 c          158 g          129 t
ORIGIN      
        1 gccgcggcat gataaaaaaa gccgccagtt gcctgagacc tctggcggtg tgagattcat
       61 ggagaatcaa ggaaagatac taccactttc gtcatcggca tttttaaatg aaaactgttt
      121 acttagtcaa ccataacggt aagaaattat gacatttatt catcaggtga tgctgtactt
      181 ctgtacggcg gtctgtgtgc tgtatcttct ttcgggtggg tacagggccg tgcgcgattt
      241 ctggcgcagg cagattgata aaagggccgc tgagaaaatc agcgccagtc agtcagccgg
      301 aagcaaaccc gaagagcccg ttactcctta acaacccctt tcagcgagaa aatcccatgt
      361 cagaaatcac atccctggtc actgctgaag cagtgaagga agtcctgcgc tctgaagaag
      421 tcctgagcgc actgaaacag aaactccgcc agaaccttga gtcgcgtctt gatgcagaag
      481 tggatgccat tctggatgaa ctgctgggcg caccggcagt tccggagccg gaaggcattg
      541 cgggtgacgg gagtgcttca gatggcggtg a
//
LOCUS       DAZFLD010000734          566 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.737, whole genome shotgun sequence.
ACCESSION   DAZFLD010000734 DAZFLD010000000
VERSION     DAZFLD010000734.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..566
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.737"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>566
                     /locus_tag="ACHKBL_005465"
     CDS             <1..>566
                     /locus_tag="ACHKBL_005465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HFO5822155.1"
                     /translation="DFENGIPVHDTIARVVSCISPAKFHECFINWMRDCHSSDDKDVI
                     AIDGKTLRHSYDKSRRRGAIHVISAFSTMHSLVIGQIRTDEKSNEITAIHELLNMLDI
                     KGKIITTDAMGCQKDTAEKIQKQGGDYLFAVKGNQGRLNKAFEEKFPLKELNNPEHDS
                     YAISEKSHGREEIRLHIVCDVPDELIDF"
BASE COUNT          184 a          107 c          126 g          149 t
ORIGIN      
        1 tgattttgaa aatggtattc ctgttcacga taccattgcc agagttgtat cctgtatcag
       61 tcctgcaaaa tttcacgagt gctttattaa ctggatgcgt gactgccatt cttcagatga
      121 taaagacgtt attgcaattg atggaaaaac gctccggcat tcttatgaca agagtcgccg
      181 caggggagcg attcatgtca ttagtgcgtt ctcaacaatg cacagtctgg tcatcggaca
      241 gatcaggacg gatgagaaat ccaatgagat cacagctatc catgaacttc ttaacatgct
      301 ggatattaaa ggaaaaatca tcacaactga tgcgatgggt tgccagaaag ataccgcaga
      361 gaagatacaa aaacagggcg gtgattattt attcgctgta aaaggaaatc aggggcggct
      421 taataaagcc tttgaggaaa aattcccact gaaagaatta aataatccag agcatgacag
      481 ttacgcaatt agtgaaaaaa gtcacggcag agaagaaatc cgtcttcaca ttgtttgcga
      541 tgtccctgat gaacttattg atttca
//
LOCUS       DAZFLD010000735          561 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.738, whole genome shotgun sequence.
ACCESSION   DAZFLD010000735 DAZFLD010000000
VERSION     DAZFLD010000735.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..561
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.738"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            185..>561
                     /locus_tag="ACHKBL_005466"
     CDS             185..>561
                     /locus_tag="ACHKBL_005466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001086080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HFO5822156.1"
                     /translation="MAKLDWKKLEQAFRREHARTGIKLQDWCRKNNISYGTARRYIKL
                     RKNPPINTEESAQKNAQKSAQIDDQKSAQKNVQGSDDESSNDKAYSDDGSGEKCAKNC
                     ANSAETKRNSRSGNPHPVARFSDR"
BASE COUNT          188 a          126 c          143 g          104 t
ORIGIN      
        1 ctcatttttc acgagatggg agcgatttcc cgcgaagccg cctgtccggt gcggtggtgg
       61 aagaaaccgg ataaaacaac cgcattgtgc aaatatcgat caaatatggt gctgctgtgt
      121 gaattctgaa aaatcacagc ggtcattatg cgtcagtttt taacacagga cgtcagaacg
      181 tgacatggca aagctggact ggaaaaagct ggagcaggca ttccgacgcg aacatgccag
      241 aactggcata aaacttcagg actggtgtcg taagaacaat atcagttatg gcacggctcg
      301 ccggtacata aaattgcgca aaaacccccc cataaataca gaggaaagtg cgcagaaaaa
      361 tgcgcaaaaa agtgcgcaaa ttgatgatca aaaaagcgca caaaaaaatg tgcagggatc
      421 cgatgacgaa tccagtaatg acaaggctta cagcgatgat ggcagtggtg aaaaatgcgc
      481 aaaaaactgc gcaaattcgg cagaaacgaa acgcaacagc agatcgggca atcctcaccc
      541 cgttgcccgg tttagcgacc g
//
LOCUS       DAZFLD010000736          551 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.739, whole genome shotgun sequence.
ACCESSION   DAZFLD010000736 DAZFLD010000000
VERSION     DAZFLD010000736.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..551
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.739"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(195..503)
                     /locus_tag="ACHKBL_005467"
     CDS             complement(195..503)
                     /locus_tag="ACHKBL_005467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000030202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone GroEL"
                     /protein_id="HFO5822157.1"
                     /translation="MSKAKFSAQAEKLKNLVDSARLQTSSSAVTATAPSQPNEGAEEV
                     SGRPVAARTKAKNLKAIPLSYFEAHAELKATSKTSLDFSSYIIEAIREKLERDGAIGQ
                     "
BASE COUNT          168 a          112 c          119 g          152 t
ORIGIN      
        1 taaaaacatc gtggacatca tggttcaggt taagggaaag gaatgaaatg gtgaaactga
       61 gaggaaagga aagcggtgac gaatcatgtc accataaaca gaaagatgtc taatggggac
      121 tctttaggta cgtgaagaat ccccataacg catttaaaag acacacatca gataccctta
      181 aggtatccga tagtttattg acctattgca ccatcacgtt caagcttctc tcttatagcc
      241 tcaataatat atgagctaaa atccagagat gttttactgg tcgctttaag ttccgcatga
      301 gcttcaaaat acgacagcgg tatggcctta agattcttag ccttcgtcct ggcagccacg
      361 ggacgacctg aaacttcctc tgcaccttca tttggctgag acggggctgt tgctgttacg
      421 gcagaagaag aagtttgtaa tctggcagaa tccaccaggt ttttcagttt ttcagcctgt
      481 gcactaaatt tcgctttgct catgagataa cccaggatgt ttgttagata cttaaaagat
      541 atccattaaa a
//
LOCUS       DAZFLD010000737          550 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.740, whole genome shotgun sequence.
ACCESSION   DAZFLD010000737 DAZFLD010000000
VERSION     DAZFLD010000737.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..550
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.740"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(1..425)
                     /locus_tag="ACHKBL_005468"
                     /pseudo
     CDS             complement(1..425)
                     /locus_tag="ACHKBL_005468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000916858.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
     gene            complement(430..540)
                     /locus_tag="ACHKBL_005469"
     CDS             complement(430..540)
                     /locus_tag="ACHKBL_005469"
                     /inference="COORDINATES: protein motif:HMM:NF033153.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HFO5822158.1"
                     /translation="MDVFTVTAATEREARAQLPHAHLIFVARIRQEASHG"
BASE COUNT          137 a          135 c          166 g          112 t
ORIGIN      
        1 cgggcgacaa aaataagatg tgcgtgcggc agccgggcgc gtgcttcgcg ctcagtggca
       61 gcggtaacgg tgaatattgc aatgctgtct gtcaggcact gcataaagcg ccagacaaag
      121 taagggcgtg cggatacagc catgtgaaag gctcccaatt taatgttaag gagtctcgct
      181 actacgctgc taaacgtggt ggcgagacgt aacagggtta gcagactggc aaattgggaa
      241 accagcgagc gcaaaggctc ccccgttacg ccccgccata atgcgggtat gggtaggttt
      301 acggacacaa aaaaaccgct tatcggatat gggcggctgt ccgccaattt attcaggctg
      361 ctaaacccgg tcgccatgtg ggcgactccg gcatcataca gcccgaaaga agaggcgatc
      421 aacatgtttt tatccatgtg atgcctcctg acggatacgg gcgacaaaaa tcagatgtgc
      481 gtgcggcagc tgcgcgcgtg cttcgcgctc agtggcagct gtaacggtga atacatcgat
      541 gttgctatgg
//
LOCUS       DAZFLD010000738          548 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.741, whole genome shotgun sequence.
ACCESSION   DAZFLD010000738 DAZFLD010000000
VERSION     DAZFLD010000738.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..548
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.741"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..131
                     /locus_tag="ACHKBL_005470"
     CDS             <1..131
                     /locus_tag="ACHKBL_005470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HFO5822159.1"
                     /translation="RHIAINILTNDNVFKAGLRRKMRKAAMDRNYLASVLAGSGLS"
     gene            142..351
                     /locus_tag="ACHKBL_005471"
                     /pseudo
     CDS             142..351
                     /locus_tag="ACHKBL_005471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310087.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          154 a          115 c          156 g          123 t
ORIGIN      
        1 tacggcacat cgctattaat attttaacga atgataatgt attcaaggca gggttaagac
       61 gtaagatgcg aaaagcagcc atggacagaa actatctggc gtcagtcctt gctgggagcg
      121 ggctttcgta atctttccct gggtgagcat ctggaactct gctgtggtaa ggcaaggctg
      181 gtgctgacta aggacggaca aatttttctc aacggcacaa aaattcattt gcagggtaag
      241 gagcaggtta atggtgactc actgttgatt aactggaact gtgcagcctc gaaatcccca
      301 ccgaagaccc ctgatgaaaa gcaggatacg ccggatatga gagagtactg atcccgtacc
      361 tgttcggtat tcagtaacaa acaagcatta aaaataaaca gaagaaggaa ttgaatcatg
      421 agcggaaaac cagcggcgcg tcagggagat atgactcagt atggcggtcc cattgtccag
      481 ggttcggcag gtgtaagaat tggcgcgccc accggcgtgg cgtgctcggt gtgtccgggc
      541 gggatgac
//
LOCUS       DAZFLD010000739          545 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.742, whole genome shotgun sequence.
ACCESSION   DAZFLD010000739 DAZFLD010000000
VERSION     DAZFLD010000739.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..545
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.742"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            332..493
                     /locus_tag="ACHKBL_005472"
     CDS             332..493
                     /locus_tag="ACHKBL_005472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5822160.1"
                     /translation="MTERSPTEIIHGIIEEQEERELILMIEEQRERQRKQEATLPESV
                     SAENQADVP"
BASE COUNT          191 a          118 c          141 g           95 t
ORIGIN      
        1 gaagcctctt cgatggggtg aaaaatccct gaaaaaatgc gcaaaaaact gcgcaaatcg
       61 atcataaaac cgatcacaaa atcgatcaca cagaccagca gagcaaagcc agatatgacg
      121 tggcttgtag ggggaaaagc caccggaaaa agtgcgcatt tttctcaaaa acgaaacgca
      181 acaggaagtg caaaacgaaa ccgatacgcg atgatcgaaa aagcgcgcaa atttaccggg
      241 ttcgggtaag caattttgcc tgatttgggc aaaatacggt gtcgccgtaa cagtgacatt
      301 gacaccaaaa cagtgacaca caggagggcc gatgaccgaa cgttcaccaa cagagataat
      361 tcacgggatt attgaagagc aggaggaacg cgagttaatc ctcatgattg aagagcagag
      421 agaacgccag cgcaaacagg aggccacgct gccagaaagc gtgagcgctg aaaatcaggc
      481 tgatgtgccg tgatggtgtc gaactgagta acttaaagtc actctgtgac cagttttaaa
      541 cgagt
//
LOCUS       DAZFLD010000740          545 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.743, whole genome shotgun sequence.
ACCESSION   DAZFLD010000740 DAZFLD010000000
VERSION     DAZFLD010000740.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..545
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.743"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            139..402
                     /locus_tag="ACHKBL_005473"
     CDS             139..402
                     /locus_tag="ACHKBL_005473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HFO5822161.1"
                     /translation="MKPNIHPEYRTVVFHDTSVDEYFKIGSTIKTDREIELDGVTYPY
                     VTIDVSSKSHPFYTGKLRTVASEGNVARFTQRFGRFVGTQKGE"
BASE COUNT          145 a          116 c          133 g          151 t
ORIGIN      
        1 tatgataaat gtgcaatcac atgtcttcca tttgatgaga ggctgcgatg ccacattacc
       61 cttgggctcg gggattctgt tgaaatgatc tagctgagaa tgtatcttat tgttatgtta
      121 taacatgagt gaagagagat gaaaccaaat atccatcctg aataccgcac agtagtgttc
      181 catgacacga gtgtggatga gtactttaaa ataggttcta ccatcaaaac agatcgcgag
      241 attgaactgg atggtgtgac gtatccgtat gtgacgattg acgtttcctc caaatcgcat
      301 ccgttctata ccggtaaact cagaacggtg gcatcagaag gcaatgttgc ccgctttact
      361 cagcgttttg gtcgtttcgt tgggactcag aagggagaat gatgagagtg agagtcttaa
      421 caggctccat gcgtggtgga tttgctccta tatttccaga catctggtat cacttaaccc
      481 attactgccc gccgccgcag atattcccgt ggagagcgat aacccagcgc actatgtgga
      541 tgcca
//
LOCUS       DAZFLD010000741          544 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.744, whole genome shotgun sequence.
ACCESSION   DAZFLD010000741 DAZFLD010000000
VERSION     DAZFLD010000741.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..544
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.744"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..213
                     /locus_tag="ACHKBL_005474"
     CDS             <1..213
                     /locus_tag="ACHKBL_005474"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5822162.1"
                     /translation="NPEIHQSPLFTLPASCQLLYKELSIPTGKRHKLNLLSQQTTLQM
                     SQEKQQTEKHSKYTAKVSVVGENPIP"
BASE COUNT          196 a          137 c           82 g          129 t
ORIGIN      
        1 aacccggaaa tccatcagag tcctttattt actttacctg cttcttgcca attactgtat
       61 aaggaattat ccataccaac tggtaagcga cacaaattaa acttattatc acaacaaaca
      121 actctgcaaa tgagtcaaga aaaacaacag acagaaaaac attcaaagta cacagcaaaa
      181 gtatctgtag tcggggaaaa tcccataccc tgacaaccca tgatgtatta ccggaaacag
      241 ggataaaagt tatgactgcc agaaggatag cagtaaaaat aaaaacacaa gttatcacaa
      301 atcactcctt gttctgaacc ggcacacaaa actgtcatat acgtttcaaa agtaaaaaca
      361 cactgctgcc acaagattca cgacgtaacc gggcagcata tcctgattac ggacaatcca
      421 tgaaaccgcc tcgccagaag cgtccatcac atccggtttt tccctgtttt atattccacg
      481 aaccatttta ttttcaggaa tctccgggcc tttatcccgc atcatataca cagctatgac
      541 aacc
//
LOCUS       DAZFLD010000742          544 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.745, whole genome shotgun sequence.
ACCESSION   DAZFLD010000742 DAZFLD010000000
VERSION     DAZFLD010000742.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..544
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.745"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>544
                     /locus_tag="ACHKBL_005475"
     CDS             <1..>544
                     /locus_tag="ACHKBL_005475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS domain-containing protein"
                     /protein_id="HFO5822163.1"
                     /translation="RYLYDPLGRRVAKRVWRRERDLTGWMSLSRKPEVTWYGWDGDRL
                     TTIQNDRTRIQTVYQPGSFTPLIRVETATGELAKTQRRSLADALQQSGGEDGGSVVFP
                     PVLVQMLDRLESEILADRVSEESRRWLASCGLTVEQIQNQMDPVYTPARKIHLYHCDH
                     RGLPLALVSTEGATEWCAEYD"
BASE COUNT          118 a          157 c          189 g           80 t
ORIGIN      
        1 cgctatcttt acgacccgct gggccgcagg gtggcaaaac gggtatggcg gcgtgaacgg
       61 gacctgacgg gctggatgtc gctgtcacgg aaaccggaag tgacctggta cggctgggac
      121 ggcgaccggc tgaccacgat acagaacgac agaacccgca tccagacggt ttatcagccg
      181 gggagcttca cgccactcat cagggtcgaa accgccaccg gagagctggc gaaaacgcag
      241 cgccgcagcc tggcggatgc ccttcagcag tccggcggcg aagacggtgg cagtgtggtg
      301 ttcccgccgg tgctggtgca gatgctcgac cggctggaaa gtgaaatcct ggctgaccgg
      361 gtgagtgagg aaagccgccg ctggctggca tcgtgcggcc tgacggtgga gcagatacaa
      421 aaccagatgg acccggtgta cacgccggcg cgaaaaatcc acctgtacca ctgcgaccat
      481 cgcggcctgc cgctggcgct tgtcagcacg gaaggggcaa cagaatggtg cgcagaatac
      541 gatg
//
LOCUS       DAZFLD010000743          527 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.746, whole genome shotgun sequence.
ACCESSION   DAZFLD010000743 DAZFLD010000000
VERSION     DAZFLD010000743.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..527
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.746"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>527
                     /locus_tag="ACHKBL_005476"
     CDS             <1..>527
                     /locus_tag="ACHKBL_005476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5822164.1"
                     /translation="YFTTKKNGKTYYIKLHDPRLMRAMKNMGPETSNAVIRTLGKVNR
                     FLATVNTSYNPEFLVSNFIRDVQTAVMNLKAEQGRSDGKLKGLDNLSALAVVKDSRSA
                     MSAVYASLRGKTLTGNGAQWQKVWKEFVEDGGKTGWFNMGDLEGQQKEMDRLVSLAKG
                     GWKGQSIGAWNSFLNL"
BASE COUNT          144 a          123 c          161 g           99 t
ORIGIN      
        1 tacttcacca ccaaaaagaa cggcaaaacg tactacatca aactccatga cccgcgcctg
       61 atgcgtgcga tgaagaatat gggaccggaa accagtaacg ccgtaatccg cacgctgggg
      121 aaagttaacc gcttcctggc aacggtgaac acgtcgtata acccggaatt cctggtcagt
      181 aacttcatcc gtgacgtaca gacggcggtg atgaacctga aggcggagca gggaaggagc
      241 gacggcaaac tgaaagggct ggataactta tccgcactgg ctgtggtgaa agacagtcgg
      301 tctgccatgt ctgccgtata cgccagtctg cgtggtaaaa ccctcacggg caacggtgcg
      361 cagtggcaga aggtgtggaa agagtttgtt gaggacggag gtaaaaccgg ctggtttaac
      421 atgggtgacc ttgaaggcca gcagaaggaa atggatcgcc ttgtctcact ggcgaagggg
      481 ggatggaaag gccagagtat cggtgcatgg aattcgttcc tgaacct
//
LOCUS       DAZFLD010000744          527 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.747, whole genome shotgun sequence.
ACCESSION   DAZFLD010000744 DAZFLD010000000
VERSION     DAZFLD010000744.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..527
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.747"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>527
                     /locus_tag="ACHKBL_005477"
     CDS             <1..>527
                     /locus_tag="ACHKBL_005477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5822165.1"
                     /translation="YFTTKKNGKTYYIKLHDPRLMRAMKNMGPETSNAVIRTLGKVNR
                     FLATVNTSYNPEFLVSNFIRDVQTAVMNLKAEQGRSDGKLKGLDNLSALAVVKDSRSA
                     MSAVYASLRGKTLTGKGAQWQKVWKEFVEDGGKTGWFNMGDLEGQQKEMDRLVSLAKG
                     GWKGQSIGAWNSFLNL"
BASE COUNT          146 a          121 c          156 g          104 t
ORIGIN      
        1 tacttcacca ccaaaaagaa cggcaaaacg tactacatca aactccatga cccgcgcctg
       61 atgcgtgcga tgaagaatat gggaccggaa accagtaacg ccgtaatccg tacgctgggg
      121 aaagttaacc gcttcctggc aacggtgaac acgtcgtata acccggaatt cctggtcagt
      181 aacttcatcc gtgacgtgca gacggcggtg atgaacctga aggctgagca gggaaggagc
      241 gacggtaaac tgaaagggct ggataactta tccgccctgg ctgtagtcaa agacagccgt
      301 tctgccatgt cagccgtata cgccagtctg cgtggtaaaa ccctcacggg caaaggtgca
      361 cagtggcaga aggtgtggaa agagtttgtt gaggacggag gtaaaaccgg ttggtttaac
      421 atgggtgacc ttgaaggcca gcagaaggaa atggatcgcc ttgtttcgct ggcgaaggga
      481 ggatggaaag gccagagtat cggtgcatgg aattcgttcc ttaacct
//
LOCUS       DAZFLD010000745          525 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.748, whole genome shotgun sequence.
ACCESSION   DAZFLD010000745 DAZFLD010000000
VERSION     DAZFLD010000745.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..525
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.748"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>525
                     /locus_tag="ACHKBL_005478"
     CDS             <1..>525
                     /locus_tag="ACHKBL_005478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HFO5822166.1"
                     /translation="AESKRFPLHEMRDDVAFQIINDELYLDGNARQNLATFCQTWDDE
                     NVHKLMDLSINKNWIDKEEYPQSAAIDLRCVNMVADLWHAPAPKNGQAVGTNTIGSSE
                     ACMLGGMAMKWRWRKRMEAAGKPTDKPNLVCGPVQICWHKFARYWDVELREIPMRPGQ
                     LFMDPKRMIEACDE"
BASE COUNT          139 a          128 c          141 g          117 t
ORIGIN      
        1 cgcggagtca aaacgatttc cgctgcacga aatgcgcgat gatgttgcat ttcagattat
       61 caatgatgaa ttatatcttg atggcaacgc tcgtcagaac ctggccactt tctgccagac
      121 ctgggacgac gaaaacgtcc ataaattgat ggatttgtcg atcaataaaa actggatcga
      181 caaagaagaa tatccgcaat ccgctgcaat cgacctgcgt tgcgtaaaca tggttgccga
      241 tctgtggcat gcgcctgcac cgaaaaatgg tcaggccgtt ggcaccaaca ccattggttc
      301 ttccgaggcc tgtatgctcg gcgggatggc gatgaaatgg cgttggcgca agcgtatgga
      361 agccgcaggc aaaccaacgg ataaaccaaa cctggtgtgc ggtccggtac aaatctgctg
      421 gcataaattc gcccgctact gggatgtgga gttgcgtgag atccctatgc gccccggtca
      481 gttgtttatg gacccgaaac gcatgattga agcctgcgac gaaaa
//
LOCUS       DAZFLD010000746          524 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.749, whole genome shotgun sequence.
ACCESSION   DAZFLD010000746 DAZFLD010000000
VERSION     DAZFLD010000746.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..524
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.749"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            109..230
                     /locus_tag="ACHKBL_005479"
                     /pseudo
     CDS             109..230
                     /locus_tag="ACHKBL_005479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            258..524
                     /locus_tag="ACHKBL_005480"
                     /pseudo
     CDS             258..524
                     /locus_tag="ACHKBL_005480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001537322.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YadA C-terminal domain-containing protein"
BASE COUNT          125 a          115 c          150 g          134 t
ORIGIN      
        1 caggtctttg aatttgttag ccagatttct gttctaattt tggatagcag aatatgcaat
       61 gttatttttc gaatcctcca taactgatat atgtgctgtt agtggagtac aagaataagc
      121 tcgcgttagc agtctgtcag tactgcgaca gcgttgtctg ggagggcttt acccggtcaa
      181 aacagcatta ttgtgcaccc catgtgacgg atgaactgtg tgctcagggg gcattttact
      241 gtaaaaaccg tgctgcggtc agccgtaacc gtcaggtggg tgaacgcaac agtcgcgcca
      301 ttgcttttca ggcacgtacc ctgcaggcgc atgagcgtcg tatgaacagc cagcagcgtc
      361 agattaatga gaatcatgag gaaatgaagc gggcagcagc ccagtcagct gcgctggccg
      421 gtcttttcca gccatacagt gtgggtaaat ttaacgccac agcggctgtg ggcggacagt
      481 gacaggcagg ctgttgcggt gggcatggga tatcgcttta acga
//
LOCUS       DAZFLD010000747          524 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.750, whole genome shotgun sequence.
ACCESSION   DAZFLD010000747 DAZFLD010000000
VERSION     DAZFLD010000747.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..524
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.750"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..183
                     /locus_tag="ACHKBL_005481"
     CDS             <1..183
                     /locus_tag="ACHKBL_005481"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5822167.1"
                     /translation="LDSLLKKTISAVLPASDKVSHQNKNKLRNNEFIKIEHPCSKSES
                     RIDGDYIISGGTEYRL"
     gene            complement(231..>524)
                     /locus_tag="ACHKBL_005482"
     CDS             complement(231..>524)
                     /locus_tag="ACHKBL_005482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraG N-terminal
                     domain-containing protein"
                     /protein_id="HFO5822168.1"
                     /translation="FWWELARWLDSWMLTALYSSDTHTRFNMMGFQNTSDDLIMNLVM
                     GTMFLVLPAVWLGALSWAGVHIGDGISRGLPNGISEAKGAASSAGSIANRGIK"
BASE COUNT          161 a          136 c           86 g          141 t
ORIGIN      
        1 ttggattcat tactaaaaaa gactatttct gctgttttac ctgcatcaga caaagtttca
       61 caccaaaata aaaacaaatt acgtaataat gaattcatta aaattgagca tccatgctca
      121 aagtctgaat ccagaatcga tggtgactac atcattagtg gaggaacaga ataccgtttg
      181 taattaagca ggagaatttt ctgtaaattc tcctgtaact tttttacttc ctattttata
      241 cctcgattag caatacttcc tgcactcgaa gctgcacctt tcgcttccga aataccattc
      301 ggtaatcctc gactgattcc atcgccaata tgaacaccag cccaggacaa cgctcccagc
      361 cacactgccg gcaagacgag aaacatcgtc cccatcacca gattcattat cagatcatcc
      421 gatgtgttct ggaatcccat catattgaac cgggtatgtg tatccgaact gtacagcgcc
      481 gtcagcatcc agctgtcaag ccaccgtgca agctcccacc agaa
//
LOCUS       DAZFLD010000748          519 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.751, whole genome shotgun sequence.
ACCESSION   DAZFLD010000748 DAZFLD010000000
VERSION     DAZFLD010000748.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..519
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.751"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>519)
                     /locus_tag="ACHKBL_005483"
     CDS             complement(<1..>519)
                     /locus_tag="ACHKBL_005483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001555725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO5822169.1"
                     /translation="FAFFAFPLSGQANDSVVVTFTGILLAKSCDITTGSKDQSVKMGT
                     YDANEFLNVGDVSPSQTFTINVQGCPTAKSTVYSSGVSANVRFTGDADAINSALLRLS
                     AGADSATGLGIEILDNNDVAIAINGESGFRDLVLNENGDANLTFKLRYKSTQENVHAG
                     QANALLYFDIDY"
BASE COUNT          170 a          124 c           84 g          141 t
ORIGIN      
        1 gataatcgat atcaaaatac aacagagcat ttgcttgccc ggcatgtaca ttttcctgag
       61 ttgatttata gcgaagttta aacgtcaaat tagcatcacc gttttcattt aacactaaat
      121 cacgaaaacc tgattcacca ttaatagcaa tcgccacatc attgttatcc aatatttcta
      181 ttcccagtcc agtcgcacta tctgcaccgg cggagagacg cagtaatgcg ctattaatgg
      241 catctgcatc accagtaaac cgcacattgg cgctcacacc agaactataa acagtacttt
      301 ttgcagtcgg gcatccttgt acattaatcg taaacgtttg actcggagaa acatcaccta
      361 catttaaaaa ttcattagca tcataagtgc ccatttttac cgactgatct ttacttccgg
      421 tagtaatgtc acagcttttt gccaacaaaa tcccagtaaa tgtaacaaca actgaatcat
      481 tggcctgccc tgagagcgga aaagcaaaaa aagcgaaaa
//
LOCUS       DAZFLD010000749          517 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.752, whole genome shotgun sequence.
ACCESSION   DAZFLD010000749 DAZFLD010000000
VERSION     DAZFLD010000749.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..517
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.752"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            186..>517
                     /locus_tag="ACHKBL_005484"
     CDS             186..>517
                     /locus_tag="ACHKBL_005484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HFO5822170.1"
                     /translation="MAKLDWKKLEQAFRREHAETGITLLDWCRKKKINYNTARTRIKM
                     GKIDHEIDHKTDHEIDHDISDEEPSNDACSDDEKCAKNSEKNCANSAETKRIRGSRLL
                     PPSNAFSQR"
BASE COUNT          174 a          115 c          119 g          109 t
ORIGIN      
        1 cctcattttt caggagacgt gagcatttac ccgcgaagcc gcctgtccgg tgcggtggtg
       61 gaagaaaccg gataaaacaa cctcattgta caaatatcga tcaagtatgg cgctgctgtg
      121 tgaattctga aaaatcacag cggtcattat gcgtcagttt ttaacagagg acgtcagaaa
      181 gtgacatggc aaagctggac tggaaaaagc tggagcaggc attccgacgc gaacatgccg
      241 aaacgggaat aacattactg gactggtgcc ggaagaaaaa gattaattac aacaccgcca
      301 gaacccgtat aaaaatgggc aaaatcgatc atgaaattga tcataaaacc gatcatgaaa
      361 tcgatcatga catctcagat gaagaaccca gcaatgacgc gtgttccgac gatgaaaaat
      421 gcgcaaaaaa ctctgaaaaa aactgcgcaa attcggcaga aacgaaacgg attcgtggtt
      481 cccgactttt acctccttca aacgcttttt ctcagcg
//
LOCUS       DAZFLD010000750          509 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.753, whole genome shotgun sequence.
ACCESSION   DAZFLD010000750 DAZFLD010000000
VERSION     DAZFLD010000750.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..509
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.753"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..350
                     /locus_tag="ACHKBL_005485"
     CDS             <1..350
                     /locus_tag="ACHKBL_005485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5822171.1"
                     /translation="KINIKNSAKFNIRSTSRLTYDIISQLHLPQNGYFMERSNFLVAI
                     IMLILRSLAIIPAWFVSLGHEIVTFPFILEIWGNAGDAFSSTSVGNYGFGHYDLNLPQ
                     SQLFAGRYGKQTK"
BASE COUNT          157 a          121 c           89 g          142 t
ORIGIN      
        1 gtaaaatcaa cataaaaaat tcagcaaaat ttaatattcg ttcaactagc cgtctcacat
       61 atgacattat tagtcaactg catttgccac aaaatggcta tttcatggaa cgcagtaatt
      121 tcttagtggc tattatcatg ctaattctta gatccctagc gattattcct gcctggttcg
      181 tcagcttagg ccatgagatt gttacattcc cttttatcct ggagatatgg gggaacgcag
      241 gcgacgcttt ttcatctaca tccgttggta actatggctt tggtcactac gacttaaacc
      301 ttcctcaatc acagctattc gccgggcgat acggcaaaca aactaagtga aacatcatcc
      361 tgaaaacacc aacatcaaca agcctctcca gatcgacatc aggagtgacc agttacaagc
      421 cacaaaccaa gaaacatatt tcatgatgag aattatgctc agcctgatgg cgggtaaaca
      481 cttattttca ccaagctcac tgagttagc
//
LOCUS       DAZFLD010000751          508 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.754, whole genome shotgun sequence.
ACCESSION   DAZFLD010000751 DAZFLD010000000
VERSION     DAZFLD010000751.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..508
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.754"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            139..>508
                     /locus_tag="ACHKBL_005486"
     CDS             139..>508
                     /locus_tag="ACHKBL_005486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000347956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HFO5822172.1"
                     /translation="MDFSTLITFSIITLSQTVSIGPGVALIISESLTYGAKKAITTSI
                     YIRFGEIVVMLVTLFSIALAINLEKHLDTIKTCGGGYLIFLGITRLIKRKGSKGNKAN
                     IPFLIPMLNPKAFLFFAAFMP"
BASE COUNT          168 a           93 c           83 g          164 t
ORIGIN      
        1 gtaatttcta ttcaatagct gccagcaatt cgcaaaaaaa catttctgac tttataatgt
       61 tgcttcctga ggcagccata cctatggagt atgtttattt taaggttcca accagaataa
      121 caggagttat ataatcccat ggacttctca acattaataa cattctctat tataacctta
      181 tcacaaacag tatcgattgg tccgggggtg gcattaatca tatctgaaag tctgacatat
      241 ggtgcaaaaa aagcaataac gacaagtata tatataagat ttggcgaaat tgttgtcatg
      301 ctagtcacac tattttctat agcactggca atcaatttag aaaaacatct tgacacaata
      361 aaaacttgtg gtggtggata tctgattttt cttggtatta ctcgtctcat caagagaaaa
      421 ggatctaaag gaaacaaagc aaatatccct tttttaatac cgatgctcaa tccaaaggcc
      481 tttttgttct ttgctgcatt tatgccat
//
LOCUS       DAZFLD010000752          507 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.755, whole genome shotgun sequence.
ACCESSION   DAZFLD010000752 DAZFLD010000000
VERSION     DAZFLD010000752.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..507
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.755"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            61..>507
                     /locus_tag="ACHKBL_005487"
     CDS             61..>507
                     /locus_tag="ACHKBL_005487"
                     /inference="COORDINATES: protein motif:HMM:NF019976.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nuclease-related domain-containing protein"
                     /protein_id="HFO5822173.1"
                     /translation="MKLPPSGEIDFLVLHPVYGVLALEVKSGIYRVEGTVFVHIKTKK
                     HVDIVKQTRNNVHGLARWLGGATTLRLRIGYGFIFPDSYFDDKIINAAMVDASVKPFR
                     GIFVDKSQLPEMAKHTIDIMQYWKHALGNNELGDERVQLIIKSVCPE"
BASE COUNT          160 a           71 c          121 g          155 t
ORIGIN      
        1 gatgatttca cagtgattca tagtctcccg tggttaagtg cggcgacacg aaatttaggg
       61 atgaaattac ctccgagtgg agaaattgat tttcttgttc tccatcctgt atatggtgtt
      121 ttggcgttag aggtaaagag tggaatatat cgcgtagaag gtactgtttt tgtacatata
      181 aaaacaaaaa aacatgtaga tatagtgaag caaacacgta acaatgtcca tggtttagcc
      241 cgttggttag ggggagcaac aacgttacgg ttgcgtattg gttatggttt tatttttcct
      301 gatagttatt tcgatgataa aattataaat gcagcaatgg tagatgcgtc tgttaaacca
      361 ttcaggggaa tatttgttga taaaagccaa ctcccggaaa tggcaaagca tactattgat
      421 ataatgcaat actggaaaca tgcattggga aataatgagt tgggggatga acgggttcaa
      481 ctaataataa aatctgtatg tcctgag
//
LOCUS       DAZFLD010000753          506 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.756, whole genome shotgun sequence.
ACCESSION   DAZFLD010000753 DAZFLD010000000
VERSION     DAZFLD010000753.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..506
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.756"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            75..182
                     /gene="ldrD"
                     /locus_tag="ACHKBL_005488"
     CDS             75..182
                     /gene="ldrD"
                     /locus_tag="ACHKBL_005488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFO5822174.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASLIVNWLNKRK"
BASE COUNT          133 a          119 c          149 g          105 t
ORIGIN      
        1 cacgggggct aatcttgaac ctagaccact caagaatagc cgcgaaacgt tgtcattaca
       61 acacaggcgg ctatatgacg ctcgcagaac tgggcatggc cttctggcat gatttagcgg
      121 ctccggtcat agctggcatt cttgccagtc tgatcgtgaa ctggctgaac aagcggaagt
      181 aacgtgtcag acgggcgtca ggctgccgta atggcaattt gcgcccggat caggccgcag
      241 ggataaaact ctgcggcctt tttcgtaaga aaaatgcaac ggttgcaagc ggataatatt
      301 ctgcggaatg cacagcacag agtgaagaag tgacggattt cggagctgaa aaacagtcgt
      361 agaatcagcg gcaagccgga aaggttccgg tgaggcgcaa tgttgcgggg gcttaatccc
      421 tggtggcatt ggttgctgga gagagaaaac ccccgcatat tgcaggtatg cacctgacaa
      481 catcacgggg gccattcttg aaccta
//
LOCUS       DAZFLD010000754          506 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.757, whole genome shotgun sequence.
ACCESSION   DAZFLD010000754 DAZFLD010000000
VERSION     DAZFLD010000754.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..506
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.757"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..483
                     /locus_tag="ACHKBL_005489"
     rRNA            <1..483
                     /locus_tag="ACHKBL_005489"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          127 a          123 c          147 g          109 t
ORIGIN      
        1 tgtcgtcagc tcgtgttgtg aaatgttggg ttaagtcccg caacgagcgc aacccttatc
       61 ctttgttgcc agcggtccgg ccgggaactc aaaggagact gccagtgata aactggagga
      121 aggtggggat gacgtcaagt catcatggcc cttacgacca gggctacaca cgtgctacaa
      181 tggcgcatac aaagagaagc gacctcgcga gagcaagcgg acctcataaa gtgcgtcgta
      241 gtccggattg gagtctgcaa ctcgactcca tgaagtcgga atcgctagta atcgtggatc
      301 agaatgccac ggtgaatacg ttcccgggcc ttgtacacac cgcccgtcac accatgggag
      361 tgggttgcaa aagaagtagg tagcttaacc ttcgggaggg cgcttaccac tttgtgattc
      421 atgactgggg tgaagtcgta acaaggtaac cgtaggggaa cctgcggttg gatcacctcc
      481 ttaccttaaa gaagcgtact ttgcag
//
LOCUS       DAZFLD010000755          505 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.758, whole genome shotgun sequence.
ACCESSION   DAZFLD010000755 DAZFLD010000000
VERSION     DAZFLD010000755.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..505
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.758"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            61..453
                     /gene="ydeI"
                     /locus_tag="ACHKBL_005490"
     CDS             61..453
                     /gene="ydeI"
                     /locus_tag="ACHKBL_005490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI family stress tolerance OB fold protein"
                     /protein_id="HFO5822175.1"
                     /translation="MKFQAIVLASFLVMPYALADDQGGLKQDAAPPPPHAIEDGYRGT
                     DDAKKMTVDFAKNMHDGASVSLRGNLISHKGEDRYVFRDKSGEINVVIPAAVFDGREV
                     QPDQMINISGSLDKKSAPAVVRVTHLQK"
BASE COUNT          155 a          101 c          123 g          126 t
ORIGIN      
        1 caattataaa aatagcaacc agacttaata agtcaaacca aacgcaatta aggagtcagt
       61 atgaaatttc aggccattgt attagcaagt tttcttgtca tgccttatgc gttggcagat
      121 gaccagggcg gattaaaaca ggatgcagcg ccaccgccgc ctcatgcaat agaagatggc
      181 tatcgcggaa ccgatgatgc aaaaaaaatg accgttgatt tcgcaaaaaa catgcacgat
      241 ggcgcttcgg tttcactacg cggtaatttg atttctcaca aaggagagga ccgttacgtt
      301 tttcgcgata agagtggtga aattaatgtt gttattcctg cggcagtctt tgatggacga
      361 gaagttcagc cggaccagat gatcaacatt agcggcagtc tggataagaa aagcgcgccg
      421 gccgtcgttc gggtcactca tttgcagaaa taaaaataaa ttagcctgat ggcctaaact
      481 actgggccat tagggtattg ggtta
//
LOCUS       DAZFLD010000756          499 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.759, whole genome shotgun sequence.
ACCESSION   DAZFLD010000756 DAZFLD010000000
VERSION     DAZFLD010000756.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..499
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.759"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(17..472)
                     /gene="tssE"
                     /locus_tag="ACHKBL_005491"
     CDS             complement(17..472)
                     /gene="tssE"
                     /locus_tag="ACHKBL_005491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000018100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HFO5822176.1"
                     /translation="MSHHSPSLYETLYGNFTGGLDLHQVCEENQVIVSVLDNMQRILN
                     TRAGSLKHLPDYGLPDMTTILQGMPGTAHQLMRVLSDVLLKYEPRIKRVDVTMQEQTQ
                     PGELHYVIDAELKDAGLVRYGTTFMPEGRVLLRHLKQQQFVDNSSYYHV"
BASE COUNT          135 a          127 c          113 g          124 t
ORIGIN      
        1 agcccggata aaaaaatcag acatgataat aagatgaatt gtccacaaac tgctgttgct
       61 taagatggcg cagcaacact ctgccctccg gcataaatgt tgttccgtaa cgaaccagcc
      121 cggcatcttt aagttctgca tcaatgacat aatgcaactc accaggctga gtctgttcct
      181 gcatcgtcac gtcgacacgc ttaatacgtg gctcatactt cagcagaaca tctgaaagca
      241 cgcgcatcag ttgatgcgcc gtacccggca ttccctgcag aatggttgtc atatccggca
      301 gcccatagtc cggaagatgt ttaaggcttc cggcccgggt attcagaata cgctgcatgt
      361 tatcgagcac cgacacgatg acctgatttt cttcacagac ctgatggagg tcgaggccgc
      421 cggtaaaatt tccgtataag gtttcgtaaa gggaggggct atgatgagac atcacgactt
      481 cttctcataa tcatttaat
//
LOCUS       DAZFLD010000757          498 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.760, whole genome shotgun sequence.
ACCESSION   DAZFLD010000757 DAZFLD010000000
VERSION     DAZFLD010000757.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..498
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.760"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>498)
                     /locus_tag="ACHKBL_005492"
     CDS             complement(<1..>498)
                     /locus_tag="ACHKBL_005492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001422120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TIGR03756 family integrating conjugative element
                     protein"
                     /protein_id="HFO5822177.1"
                     /translation="SHYIPEVVVSTYTAPGGNPWREMSAVSSTAGGPENAISKGIAGL
                     SAGGGNPADMKVQGERRSSIRFKYADATGHPATTLIGGMVPGYSCESPVTPFVPYFLS
                     TLDSIAWRTGLPESLYPEALIPGKREIGSQEEENMWGNVYPRSGFVTQTDDDKAAAVV
                     AQRVA"
BASE COUNT          107 a          151 c          127 g          113 t
ORIGIN      
        1 tcagccaccc gctgggccac caccgcagca gctttatcat catctgtctg ggtcacgaac
       61 ccggagcggg gatacacatt gccccacata ttctcttctt cctggctgcc tatttcgcgt
      121 ttaccgggga tcagggcctc cgggtacagt gactccggta gtcctgtacg ccaggcaata
      181 ctgtcaagcg tactgagaaa ataaggaaca aaaggtgtca ccggtgactc acaggaatat
      241 cccggcacca tccctccgat aagcgtggtg gccgggtgcc ctgttgcatc ggcatattta
      301 aaacggattg acgaacgacg ttccccctgt accttcatgt ctgcggggtt ccctcctcct
      361 gctgacagac cggcaatacc tttgcttatg gcattctccg gaccaccggc tgtactgctg
      421 accgccgaca tttcacgcca gggatttccg ccaggtgcgg tataggtgga aacaaccact
      481 tccgggatat aatgggaa
//
LOCUS       DAZFLD010000758          497 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.761, whole genome shotgun sequence.
ACCESSION   DAZFLD010000758 DAZFLD010000000
VERSION     DAZFLD010000758.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..497
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.761"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            2..433
                     /locus_tag="ACHKBL_005493"
     CDS             2..433
                     /locus_tag="ACHKBL_005493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
                     /protein_id="HFO5822178.1"
                     /translation="MSSGCSSQPSIGNKAQTAHIDQIMIYEFMHGLNRDQNVNHHHLT
                     IKKPIDKASPLLGKAICDNELLTCDFSFYRTNKFGINELYYKIKLTGAKISDIHVSIS
                     HIVVDNSIQPEESVSFSYESIIWEHCSAGTSAYSLWEDRLF"
BASE COUNT          178 a           86 c           77 g          156 t
ORIGIN      
        1 catttcaagt ggttgttcat cacaaccatc cattggaaat aaagctcaaa cagcacacat
       61 tgatcaaatt atgatttatg aattcatgca tggattaaat cgagatcaaa acgtcaacca
      121 tcatcatttg acaataaaaa aacctataga taaagcatca cccttattag gcaaagcaat
      181 ttgcgacaat gaattattga catgtgactt ttccttttat cgtacaaata aatttggaat
      241 taatgaactg tactataaaa tcaaattaac tggagcaaag ataagcgaca ttcatgtctc
      301 tatatcacat attgttgttg ataacagcat tcaacccgaa gagtcagttt ctttcagtta
      361 tgagtcaatt atatgggagc attgttccgc tggcactagt gcatacagtt tatgggaaga
      421 tcgtcttttt taaaaatatc tgggattatt ataatggcta acaatatttt acactcttca
      481 acatcagaca gtgaaag
//
LOCUS       DAZFLD010000759          496 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.762, whole genome shotgun sequence.
ACCESSION   DAZFLD010000759 DAZFLD010000000
VERSION     DAZFLD010000759.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..496
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.762"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..183
                     /locus_tag="ACHKBL_005494"
     CDS             <1..183
                     /locus_tag="ACHKBL_005494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HFO5822179.1"
                     /translation="DGIVHNSFGQKLMRIYNQKGIFSNTKDSEEGLTHILSEHFENVK
                     TKVQGTVVMFSASGKK"
BASE COUNT          151 a          115 c           83 g          147 t
ORIGIN      
        1 gatggaattg tgcacaatag cttcggtcaa aaactgatgc gcatttacaa tcagaaaggc
       61 atcttttcaa acacaaaaga ttccgaagaa ggcttaacac atatactctc agagcatttc
      121 gagaatgtta aaaccaaggt tcaaggtact gtagtaatgt tttccgcttc agggaaaaaa
      181 tagcatccaa ccgcagcacg ttcttgcata aggcgtgtct gcggtttttc aactattcag
      241 atacatcact cccatcacat tcattcctcc gcatcatagg catataggct atatcacctt
      301 gatatttttc ttcttcagat aaaaactgtt atctatgtat acttttaaac ccaatccgtg
      361 tagagtctct gcataagata gtttgcagtt gacacttcag cttgcgccat aaaccgcctg
      421 atttttgccg ccacctgtta gcattcctgt atacctgaaa cgacaatgtt tatctacgaa
      481 ctttaagaac acccag
//
LOCUS       DAZFLD010000760          495 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.763, whole genome shotgun sequence.
ACCESSION   DAZFLD010000760 DAZFLD010000000
VERSION     DAZFLD010000760.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..495
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.763"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            101..>495
                     /locus_tag="ACHKBL_005495"
     CDS             101..>495
                     /locus_tag="ACHKBL_005495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5822180.1"
                     /translation="MLTKKMLLVCASCVALSACSAQEIAELNRQISDGAFAVTKVLNV
                     GVNQADDDLKYLSQAPRAKREKRDHVIEVPVDVDTVAGRLKRYYKFYSNEEVDAIRNN
                     GHANGKWAAAAISQNGHMWDAMPGSYYKMG"
BASE COUNT          147 a           83 c          139 g          126 t
ORIGIN      
        1 gctgaatgaa taaaatcttc atctggtgtt tttgttgcgc gtaaatgcag gtgtaactgt
       61 ggatgttatc cactgaagag ctgctgatga ggatgaagac atgctgacta aaaaaatgtt
      121 actcgtttgt gcttcgtgtg ttgcgctgag tgcctgttct gcacaggaaa ttgcagagct
      181 gaacaggcag ataagtgacg gtgcatttgc tgtgacaaaa gtactgaacg taggtgtaaa
      241 ccaggcagat gatgatctca aatatctgtc tcaggcaccc cgggcaaaaa gagagaaacg
      301 tgatcatgtc atagaagtcc ctgttgatgt ggatacagtt gcgggaagac tgaaacggta
      361 ctataagttt tattcaaatg aagaagtcga tgcgattcgt aataacggac atgcgaacgg
      421 gaagtgggcc gcagcagcaa taagtcagaa cgggcatatg tgggatgcca tgcccggtag
      481 ttattataaa atggg
//
LOCUS       DAZFLD010000761          492 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.764, whole genome shotgun sequence.
ACCESSION   DAZFLD010000761 DAZFLD010000000
VERSION     DAZFLD010000761.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..492
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.764"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>492
                     /locus_tag="ACHKBL_005496"
     CDS             <1..>492
                     /locus_tag="ACHKBL_005496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5822181.1"
                     /translation="PADSNLNATGSNKRKYGLVTLKGLPEKTSSTLTPGQRYWVVVDN
                     NNVKPAAGAGPGWWRHLLPPAKEVMVFDKTVSLRSPFAIKAGDPIGHMGYYQAPKDGG
                     YEARYQVHIECTSMDDNLEKFLTNPEQVGKNNPLWLKYAPGLALYKKDVSTGTFTKDT
                     GVT"
BASE COUNT          141 a          114 c          138 g           99 t
ORIGIN      
        1 acccggcaga cagcaatctt aacgctacag gaagtaacaa gagaaaatac ggtcttgtga
       61 cgctaaaggg tttaccagaa aaaaccagca gtactctcac tcccggtcag cgctactggg
      121 ttgttgtgga taacaacaat gttaagccag cggcgggggc cgggcccggc tggtggcgac
      181 acttgctgcc tcccgcaaag gaagtcatgg tattcgataa aacagtaagc ctccgttccc
      241 cattcgccat taaggctggt gacccgattg ggcacatggg ctactatcag gcaccaaagg
      301 atggggggta tgaggcacga tatcaggtac atatcgaatg cacgagtatg gatgataatc
      361 tggagaaatt cctgaccaat cctgagcagg tgggaaaaaa taatccactc tggctgaagt
      421 acgccccggg acttgcgttg tacaagaaag atgtttctac aggaacattc acgaaggata
      481 cgggggtgac gg
//
LOCUS       DAZFLD010000762          492 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.765, whole genome shotgun sequence.
ACCESSION   DAZFLD010000762 DAZFLD010000000
VERSION     DAZFLD010000762.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..492
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.765"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(93..>492)
                     /locus_tag="ACHKBL_005497"
     CDS             complement(93..>492)
                     /locus_tag="ACHKBL_005497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5822182.1"
                     /translation="NTLPVNATYASEPTVDTQKFSDILYSAGCSLKDLAYLSRCLASI
                     HPTIAKNIYETENLSDQKLLHIDCRSTNEIKINIFFGQQREGLIEINSDTVIFSILSS
                     LIDSTISKFQHQNPVNGSVNPDLLYEDYTD"
BASE COUNT          145 a           76 c          113 g          158 t
ORIGIN      
        1 gtacatgaag gtgctaccag tacgagaaac gttaatcaaa agttttatta cgtctggcaa
       61 taacgcaggc catttttcgc gacaggaggg atttaatcag tataatcctc atacaatagg
      121 tcaggattta cagaaccgtt aacggggttt tggtgttgaa atttagaaat ggtggagtca
      181 atcaatgaag acagtatcga aaaaatgact gtgtcagaat taatttcaat taatccttct
      241 cgttgttgac caaaaaagat atttatcttg atttcatttg tactacggca atcaatatgt
      301 agtaattttt gatcgctgag attttctgtt tcataaatgt ttttggcgat agtcggatgt
      361 attgatgcca gacatctgct cagataagcc agatccttta gggaacaccc cgctgagtag
      421 aggatgtcag agaatttttg tgtgtcaact gttggctctg aagcgtatgt tgcattgact
      481 ggaagggtgt tg
//
LOCUS       DAZFLD010000763          484 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.766, whole genome shotgun sequence.
ACCESSION   DAZFLD010000763 DAZFLD010000000
VERSION     DAZFLD010000763.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..484
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.766"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(183..>484)
                     /locus_tag="ACHKBL_005498"
     CDS             complement(183..>484)
                     /locus_tag="ACHKBL_005498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015386758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Imm52 family immunity protein"
                     /protein_id="HFO5822183.1"
                     /translation="NNNSWIKVDSKGYWRNDSNVFPDRVYVGWMLYMPHILLPELIPE
                     AAKIVPVFEGEKQKGTIVVSTEDVFDGNNKEHIGKANDIEIRLLDLGLLPLLTEL"
BASE COUNT          129 a          103 c           88 g          164 t
ORIGIN      
        1 actttggttc ccactactta tttggtggac accactttgt ctaattcgtc agattctgac
       61 cagacggttc aggctgtacg cttaccttaa tggtgagcat tcaattttat ctgatgtgtt
      121 aaaaataagg ccattaacgg gtgtaactgt cccgtcatct acccatgagt gataaggttg
      181 gattaaagct ctgttagcaa aggtagcagt ccaagatcca gaagtcttat ttcaatatca
      241 ttagcttttc cgatgtgttc cttattattt ccgtcaaata cgtcttcggt tgaaacaaca
      301 atagttccct tttgcttctc gccctcgaaa accggtacta tttttgcggc ttcagggatt
      361 aattcaggta agagtatgtg gggcatatat aacatccatc ccacatatac cctgtcagga
      421 aaaacattac tgtcattacg ccaataacct tttgagtcga cttttatcca tgaattatta
      481 tttt
//
LOCUS       DAZFLD010000764          481 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.767, whole genome shotgun sequence.
ACCESSION   DAZFLD010000764 DAZFLD010000000
VERSION     DAZFLD010000764.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..481
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.767"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..449)
                     /locus_tag="ACHKBL_005499"
     CDS             complement(<1..449)
                     /locus_tag="ACHKBL_005499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HFO5822184.1"
                     /translation="MRGSVIIPVALHALVAVSTLLWLLWYFIPAGNVFNGLATLLILL
                     LAGWSVFKNSRSEKQKLTSDVTLTDAEPALSDTRGPVVLVCGDMPEALFQDGPLRKTA
                     RGWWLRVGDVSRLTDVVRSIQTQFPRQVGQLSVMYRCLPDCHHDEAVL"
BASE COUNT          125 a          140 c          126 g           90 t
ORIGIN      
        1 agaaccgctt catcatgatg acagtctggc aggcagcggt acatcacaga gagctgaccg
       61 acctggcgcg ggaactgcgt ctgtatgctg cggacaacgt cagttaagcg actgacgtca
      121 ccgacccgca gccaccatcc ccgggctgtt ttacgcagcg gaccgtcctg aaacaacgct
      181 tccggcatgt ctccacatac cagtacaaca gggccccggg tgtcggataa tgccggttca
      241 gcatccgtca gggtaacgtc agatgtgagc ttttgctttt cactccggct gtttttaaaa
      301 acactccagc ctgccagaag aaggatcagc agagtagcca gaccgttaaa aacgttgcct
      361 gccgggatga aataccacag caaccacagc aacgtggaaa cggccaccag ggcatgcagg
      421 gctaccggaa tgatgacaga tccacgcatc agccatgcaa ctccggtatt tgtgatgaca
      481 a
//
LOCUS       DAZFLD010000765          478 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.768, whole genome shotgun sequence.
ACCESSION   DAZFLD010000765 DAZFLD010000000
VERSION     DAZFLD010000765.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..478
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.768"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..375)
                     /locus_tag="ACHKBL_005500"
     CDS             complement(<1..375)
                     /locus_tag="ACHKBL_005500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000332798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5822185.1"
                     /translation="MCDFIKSCYAAPRINKMADFFSSTASGVKHKPLKHVKIDNRLFP
                     SPEYIIFSDYLTKNSGPFVNHYFCSIPYSKEEECRLGVTIMKYASHRNKPLSLWCLGM
                     AEGAMARTISQLSKGQVLSLTTS"
BASE COUNT          146 a           85 c           80 g          167 t
ORIGIN      
        1 gctggttgtt aatgataaaa cctgtccttt tgatagttga gaaattgttc tggccatggc
       61 tccttctgcc attccaagac accataagct caatggctta tttcgatggc ttgcatattt
      121 cataatagtc acacccagcc tgcattcttc ttctttgctg tatggaatac tgcaaaagta
      181 gtggttaaca aatggaccac tgtttttcgt aagatagtca gaaaaaatta tatactcagg
      241 agatgggaaa agcctgttat caatttttac atgcttcaaa ggtttatgtt ttactccaga
      301 tgctgttgat gagaaaaaat cagccatttt gttaattctt ggtgctgcat aacatgattt
      361 tataaaatca cacatttaac tatcctgtat aacttataac atcagtaaat caggagcata
      421 tactattttt ctgaagtatc aaacagaatc tgcttatttg aaataagctg ttatctct
//
LOCUS       DAZFLD010000766          477 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.769, whole genome shotgun sequence.
ACCESSION   DAZFLD010000766 DAZFLD010000000
VERSION     DAZFLD010000766.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..477
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.769"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..195
                     /locus_tag="ACHKBL_005501"
     CDS             <1..195
                     /locus_tag="ACHKBL_005501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO5822186.1"
                     /translation="IPTNGATIPNPETTRETYRKDEVIPLQRKIMNAVNSDPDIPEHL
                     YLRFDVEAGHVAEEKTVPVG"
     gene            233..>477
                     /locus_tag="ACHKBL_005502"
     CDS             233..>477
                     /locus_tag="ACHKBL_005502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001217942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ogr/Delta-like zinc finger family protein"
                     /protein_id="HFO5822187.1"
                     /translation="MRIFKARCPDCGAPAVIRKTDWKTPELADLYCACSDVECGHTFV
                     CTMTFSHTLSPGARSGRNMVKFLVDAMRPEDRRYALEL"
BASE COUNT          110 a          129 c          133 g          105 t
ORIGIN      
        1 atccccacga acggggccac catccctaac ccggaaacga cgcgtgaaac gtaccgcaag
       61 gatgaggtga taccgcttca gcgtaaaatc atgaacgccg tgaacagcga tcccgatata
      121 ccggaacacc tttatcttcg gtttgatgtg gaagccgggc atgtggcgga agaaaaaacg
      181 gtgccggtcg ggtaaaatgt cttccccggg atacgtgtta tcgggaggta agatgcggat
      241 ttttaaagcc cggtgtcctg actgtggcgc acctgctgtc atccggaaga cagactggaa
      301 aacccctgaa cttgcggatc tgtactgtgc atgcagtgat gtcgaatgcg ggcatacctt
      361 cgtctgtacg atgacctttt cccataccct cagccccggc gcccgctccg gtcgcaatat
      421 ggtgaagttt cttgttgatg ccatgcgccc ggaagatcgc cggtatgcgc ttgaact
//
LOCUS       DAZFLD010000767          477 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.770, whole genome shotgun sequence.
ACCESSION   DAZFLD010000767 DAZFLD010000000
VERSION     DAZFLD010000767.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..477
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.770"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..150)
                     /gene="yciX"
                     /locus_tag="ACHKBL_005503"
     CDS             complement(<1..150)
                     /gene="yciX"
                     /locus_tag="ACHKBL_005503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YciX"
                     /protein_id="HFO5822188.1"
                     /translation="MVGQEQLESSPLCQHSDNETETKRECSVVIPDDWQLTSQQQAFI
                     ELFAED"
     gene            complement(153..293)
                     /gene="ymiA"
                     /locus_tag="ACHKBL_005504"
     CDS             complement(153..293)
                     /gene="ymiA"
                     /locus_tag="ACHKBL_005504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588449.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmiA family putative membrane protein"
                     /protein_id="HFO5822189.1"
                     /translation="MRLAMPSGNQEPRRDPELKRKAWLAVFLGSALFWVVVALLIWKV
                     WG"
BASE COUNT          141 a          111 c           96 g          129 t
ORIGIN      
        1 atcttcagca aacagttcta taaaggcttg ctgctgcgat gttagttgcc agtcgtctgg
       61 aataacgacg gaacattccc gtttcgtttc tgtttcattg tcactatgct gacataatgg
      121 tgacgactcc agttgctcct gaccgaccat agttatcccc acactttcca aatcagcagt
      181 gcgacaacca cccagaaaag agcagaacca agaaataccg ccagccaggc tttgcgtttt
      241 aattcagggt cccggcgggg ttcctgattt ccagaaggca ttgctaacct catataatcg
      301 ccatcactta tcagcaagac gttgaacgat tggtgtaatt aatcatacga tgagacaaat
      361 cctaaagaaa ctttagtcaa aaatccagcg aatttacgaa tcgtctccag tgaataattc
      421 aatcttttga cctgaaatag atatttgaaa actcgagttt gcgaggcggg taattag
//
LOCUS       DAZFLD010000768          473 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.771, whole genome shotgun sequence.
ACCESSION   DAZFLD010000768 DAZFLD010000000
VERSION     DAZFLD010000768.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..473
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.771"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(165..>473)
                     /gene="hcp"
                     /locus_tag="ACHKBL_005505"
     CDS             complement(165..>473)
                     /gene="hcp"
                     /locus_tag="ACHKBL_005505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system effector Hcp"
                     /protein_id="HFO5822190.1"
                     /translation="TKEIDSSSPYLYKAVTTGQTLKSAEFKWYRINDAGQEVEYFNTK
                     LENVKVVKVNPVMHDIKNPAYEKHNHLEQVELRYEKITWTYKDGNIIHSDAWNERTTA
                     "
BASE COUNT          112 a          128 c          108 g          125 t
ORIGIN      
        1 ttcactcact gtcagaagac cttcagacac gctaccgccc ttcgccataa cccgggcgca
       61 tcaggtctgt gttcaggatt gagaattctt tcttcagcac ccgccaacag atactgaaga
      121 aaaaagaaaa atgacggaca ggataccctg cccgtccggg agaattacgc agtggtacgt
      181 tcattccacg cgtcagaatg aatgatgtta ccgtccttat aagtccaggt gattttctcg
      241 tagcgcagct ccacctgctc aaggtggtta tgcttctcgt aggcgggatt cttgatgtcg
      301 tgcatgaccg ggttaacctt caccaccttc acgttctcaa gcttcgtatt gaaatactcc
      361 acttcctgac cggcatcatt gattctgtac cacttaaatt cagcagactt cagggtctgc
      421 cccgtggtca ccgccttgta aaggtagggg ctggaggaat caatttcctt ggt
//
LOCUS       DAZFLD010000769          461 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.772, whole genome shotgun sequence.
ACCESSION   DAZFLD010000769 DAZFLD010000000
VERSION     DAZFLD010000769.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..461
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.772"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            5..>461
                     /locus_tag="ACHKBL_005506"
     CDS             5..>461
                     /locus_tag="ACHKBL_005506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001282578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFO5822191.1"
                     /translation="MLPGLTTTRKPIVTKKLSEIVAEELERMIRQEEIREGEYLPSER
                     ELMAFFGVGRPSVREALSSLRHKGLIRYNKGEKASVCRPSPETIINGLSGIVKDFLST
                     ADGIKDFERLRIFFETNLVRYAAKNATPEDIHRLEKVLSIHHNSLRTPEV"
BASE COUNT          149 a           98 c          102 g          112 t
ORIGIN      
        1 aaccgtgtta cctgggctaa caacgaccag aaagcccatt gttacaaaaa aactttcaga
       61 aatcgttgcc gaagagcttg aaagaatgat ccgccaggag gagataaggg aaggtgaata
      121 cctgccttct gaacgtgaac ttatggcatt ctttggcgtt ggaagaccat ctgtcagaga
      181 ggctttatca tctctcagac ataaagggct aatccggtat aacaagggag aaaaagccag
      241 tgtctgccgt ccatcaccag aaaccatcat taatgggtta tcaggcattg ttaaagattt
      301 tttatccaca gctgatggca tcaaggattt tgaaagacta cgcatctttt ttgaaaccaa
      361 ccttgtaaga tatgcagcaa aaaatgcaac gcctgaggat attcacaggc ttgagaaggt
      421 gctcagtatc catcataact cactgagaac ccccgaagtg t
//
LOCUS       DAZFLD010000770          456 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.773, whole genome shotgun sequence.
ACCESSION   DAZFLD010000770 DAZFLD010000000
VERSION     DAZFLD010000770.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..456
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.773"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..438)
                     /locus_tag="ACHKBL_005507"
     CDS             complement(<1..438)
                     /locus_tag="ACHKBL_005507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000532789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5822192.1"
                     /translation="MGRKLIWRAVASAPGHEEVHVFVDASDRAEAKKHLYQRLAEEWR
                     CSVNRITFHNMWNENELREMSTGPREPAGLPLLESGGGNGGVFYDHEPLLLIASRRLR
                     ASLETALIVLQKWTVCVGCGYSGVRDGAGKNAPVWVKVNPESGL"
BASE COUNT          103 a          149 c          101 g          103 t
ORIGIN      
        1 cagaccactc tcaggattca ccttcaccca gaccggagca ttcttaccgg caccatcccg
       61 taccccggaa taaccacagc ccacgcacac cgtccacttc tgcagtacga tgagtgcagt
      121 ttcgagtgat gcgcggagac gccgggaggc aataaggagc aacggctcat ggtcataaaa
      181 aaccccgccg ttaccccctc cggattcaag cagcgggagt cctgcaggct cccggggacc
      241 tgttgacatt tcccgtaatt cgttttcatt ccacatattg tggaaagtta tccggttcac
      301 tgaacaacgc cattcctccg ccagacgctg atacaaatgc tttttcgctt ctgcgcggtc
      361 agatgcatca acaaacacat gcacttcctc gtgtccgggt gctgaagcca cggcacgcca
      421 tatcagtttt ctgcccacag gcttttccct ccgttt
//
LOCUS       DAZFLD010000771          450 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.774, whole genome shotgun sequence.
ACCESSION   DAZFLD010000771 DAZFLD010000000
VERSION     DAZFLD010000771.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..450
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.774"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..129)
                     /locus_tag="ACHKBL_005508"
     CDS             complement(<1..129)
                     /locus_tag="ACHKBL_005508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5822193.1"
                     /translation="MIYITIFMILPCPVPCSHVFLYVFYIFLFLVLFIMTIYQSSKK"
     gene            211..>450
                     /locus_tag="ACHKBL_005509"
     CDS             211..>450
                     /locus_tag="ACHKBL_005509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HFO5822194.1"
                     /translation="MSIQSLLDYISVIPDIRQQGKVKHKLSDILFLTVCAVIVGADEW
                     QEIEDFGHERLEWLKKYGDFDNGIPVDDTIAPGKDH"
BASE COUNT          164 a           64 c          100 g          122 t
ORIGIN      
        1 ttttttactg gattggtaaa tggtcataat gaacaacacc agaaagagaa aaatataaaa
       61 tacatacaga aagacatgag agcaagggac ggggcagggc aagatcatga aaattgtgat
      121 gtaaatcacg attttcatcc tggctttaac gcctacaggt gatcaaaatt taacctttgg
      181 aataactaaa aagataaaaa aggacgccag gtgagtattc aaagtttgct tgattatatt
      241 tcagtgatcc ctgatatacg acaacaagga aaggttaaac ataaattatc tgatattttg
      301 tttctcaccg tatgtgcagt aattgtaggt gccgatgagt ggcaggaaat tgaagatttt
      361 ggacatgaaa gacttgaatg gctaaagaaa tatggtgatt ttgataatgg cattccggtc
      421 gatgacacca ttgcaccagg gaaagatcat
//
LOCUS       DAZFLD010000772          448 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.775, whole genome shotgun sequence.
ACCESSION   DAZFLD010000772 DAZFLD010000000
VERSION     DAZFLD010000772.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..448
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.775"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..161)
                     /locus_tag="ACHKBL_005510"
     CDS             complement(<1..161)
                     /locus_tag="ACHKBL_005510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tube protein Hcp"
                     /protein_id="HFO5822195.1"
                     /translation="MAIPVYLWLQDDGGADIKGSVDVKDREGSIEVVAQEHCLYIPTD
                     NNTGKLTGTR"
     gene            complement(167..>448)
                     /locus_tag="ACHKBL_005511"
     CDS             complement(167..>448)
                     /locus_tag="ACHKBL_005511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000558483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HFO5822196.1"
                     /translation="HTDNTGDPQRNQALSLKRAEAVRDWMRDTGDIPQSCFAVQGYGE
                     SRPVAPNDSAEGRARNRRVEISLVPQADACRLPDASPAPEEGASENITE"
BASE COUNT           89 a          144 c          117 g           98 t
ORIGIN      
        1 cgggtaccgg tcagcttacc ggtattgtta tccgtcggaa tgtaaaggca gtgctcctga
       61 gccaccacct caatgctgcc ctcacggtcc ttcacatcca cggagccttt aatatccgct
      121 ccgccatcat cctgcagcca cagataaaca ggaattgcca taaaaatcac tccgttatgt
      181 tttctgatgc gccctcttcc ggcgcagggg aagcgtccgg cagacgacag gcatcggcct
      241 gcggtaccag actgatttca acacggcggt tacgcgcgcg gccttccgcc gagtcattcg
      301 gggccacagg gcggctttca ccgtatccct gcacggcaaa acagctttga gggatatcgc
      361 ccgtatcccg catccagtca cgaaccgcct ctgcacgctt cagggacaga gcctggttgc
      421 gttgcgggtc acctgtattg tccgtgtg
//
LOCUS       DAZFLD010000773          448 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.776, whole genome shotgun sequence.
ACCESSION   DAZFLD010000773 DAZFLD010000000
VERSION     DAZFLD010000773.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..448
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.776"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>448
                     /locus_tag="ACHKBL_005512"
     CDS             <1..>448
                     /locus_tag="ACHKBL_005512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002431274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligogalacturonate-specific porin KdgM family
                     protein"
                     /protein_id="HFO5822197.1"
                     /translation="CSQALTLNLRGGYRSASHAYETLIQVSQGWNNGWWASFESNTWN
                     TIHNNENELFALNYNKIDINKTYKIDDKWALIPGIQTQWMSAGSQYNPYLKISYQATP
                     DLNLALRYRYDWKAFRQTDLDGNRARNDQHQFDGYVTYKVNDSWLFA"
BASE COUNT          149 a           76 c           99 g          124 t
ORIGIN      
        1 tgctctcagg cattaactct taatctacgt ggaggataca gaagcgccag tcacgcttat
       61 gaaacgttga ttcaggttag tcaggggtgg aataatggtt ggtgggcaag ttttgagtca
      121 aatacctgga atacaattca caacaatgaa aatgaattgt ttgcactaaa ttataataaa
      181 atagatataa ataaaacata taaaatagat gacaaatggg ctctaatccc agggattcag
      241 acacaatgga tgtcagcagg aagtcagtat aacccttatc tgaaaatttc gtatcaggca
      301 acgccagatc ttaatctggc cttacgctat cgctatgact ggaaagcttt ccgacagacg
      361 gatttagatg gcaacagggc gcgtaatgat cagcatcagt ttgatggtta tgtgacatac
      421 aaagttaatg attcatggtt gtttgcat
//
LOCUS       DAZFLD010000774          447 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.777, whole genome shotgun sequence.
ACCESSION   DAZFLD010000774 DAZFLD010000000
VERSION     DAZFLD010000774.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..447
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.777"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..161)
                     /locus_tag="ACHKBL_005513"
     CDS             complement(<1..161)
                     /locus_tag="ACHKBL_005513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tube protein Hcp"
                     /protein_id="HFO5822198.1"
                     /translation="MAIPVYLWLQDDGGADIKGSVDVKDREGSIEVVAQEHCLYIPTD
                     NNTGKLTGTR"
     gene            complement(196..>447)
                     /locus_tag="ACHKBL_005514"
     CDS             complement(196..>447)
                     /locus_tag="ACHKBL_005514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000558483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HFO5822199.1"
                     /translation="HTDNTGDPQRNQALSLKRAEAVRDWMRDTGDIPQSCFAVQGYGE
                     SRPVAPNETAEGRARNRRVEISLVPQADACRLPDITRAP"
BASE COUNT           84 a          148 c          114 g          101 t
ORIGIN      
        1 cgggtaccgg tcagcttacc ggtattgtta tccgtcggaa tgtaaaggca gtgctcctga
       61 gccaccactt caatactgcc ctcacggtcc ttcacatcca cggagccttt aatatccgct
      121 ccgccatcat cctgcagcca cagataaaca ggaattgcca taaagttact ccctctcgtt
      181 gtcttacgcg ccctgttacg gcgcccgggt gatgtccggc agacgacagg catcggcctg
      241 cggtaccaga ctgatttcaa cacggcggtt acgcgctcgc ccttccgccg tctcattcgg
      301 agccacggga cgactctcac catatccctg tacggcaaaa cagctttgag ggatatcgcc
      361 cgtatcccgc atccagtcgc gaacggcctc tgcgcgcttc agtgacaggg cctggttgcg
      421 ctgcgggtcg cccgtattgt ccgtgtg
//
LOCUS       DAZFLD010000775          446 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.778, whole genome shotgun sequence.
ACCESSION   DAZFLD010000775 DAZFLD010000000
VERSION     DAZFLD010000775.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..446
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.778"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            26..409
                     /locus_tag="ACHKBL_005515"
     CDS             26..409
                     /locus_tag="ACHKBL_005515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc ribbon protein"
                     /protein_id="HFO5822200.1"
                     /translation="MYAKSFIAFDGNGRLTGARTAQTAPYDRYTCHLCGSALRYHPQY
                     DTERPWFEHTDDGLTEHGQQCPYVRPERREIQLIKRLQQFVPDALPVVRKASWHCRQC
                     HHDYYGEQYCTHCQTGRFSEEGGAE"
BASE COUNT          110 a          120 c          131 g           85 t
ORIGIN      
        1 cgtctggcgg ctgaggaata tgacgatgta cgcaaaatcc tttattgcat ttgatggcaa
       61 cgggcgtctg acgggtgccc gtaccgcaca gactgcacct tacgatcgct acacttgcca
      121 cctttgcggc agtgcgctca gataccaccc gcaatacgac actgagcgtc cctggtttga
      181 acacactgac gacgggctga cggagcacgg tcagcagtgc ccttatgtca gaccggaacg
      241 cagggagata caactgatta aacgtctgca gcaattcgtc ccggatgcct tacccgtagt
      301 acgtaaagcc agctggcact gcaggcaatg tcaccacgat tattatgggg agcagtactg
      361 cacacactgc cagaccgggc gcttcagtga ggagggggga gcagaatgaa cggtataaaa
      421 acggggctgg ggatcacgcc gggtga
//
LOCUS       DAZFLD010000776          442 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.779, whole genome shotgun sequence.
ACCESSION   DAZFLD010000776 DAZFLD010000000
VERSION     DAZFLD010000776.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..442
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.779"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
BASE COUNT          128 a           92 c          101 g          121 t
ORIGIN      
        1 acttgatttg acaggaacac attcaaaaaa atttctttat tcatttcagg agcgattttc
       61 atctgttact tgaagtatct gtgagattaa gtgactcccc ctaacgtcgt actaaatagt
      121 aattactgtc cataccggga gcagtattcg gcgctcccca catgcagcag aataaccgca
      181 tcagcgaaga tgtattgtat ttttactatc acaatgtgta cggacggttt atggaaaaga
      241 atgaaaatta gatacgcgat ggggcggcag gttacacggt tatcactacg atagccaata
      301 ctggctggtg cattaccgcc gtgaacaata ggacgccgta ctgctggaaa tttcttacct
      361 ttatgactcc ccgcgccgcc gtattgggaa acgggaagca atcaagatga ggctgatata
      421 atgtgcggaa tgcctgagta tt
//
LOCUS       DAZFLD010000777          438 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.780, whole genome shotgun sequence.
ACCESSION   DAZFLD010000777 DAZFLD010000000
VERSION     DAZFLD010000777.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..438
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.780"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..387
                     /locus_tag="ACHKBL_005516"
     CDS             <1..387
                     /locus_tag="ACHKBL_005516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion protein Rhs"
                     /protein_id="HFO5822201.1"
                     /translation="GTSSIDITGSGDLISLGGHAGLGVAFAKKKGEMLSDICIYATAC
                     GHAGIGGGINAAITYSETKSLPTSGVSNSVGVTVGGGVGGHFAYTYVVDVDNPESSTE
                     SVGIGAGVDASVMTLACRTWQECWVN"
BASE COUNT          120 a           58 c          119 g          141 t
ORIGIN      
        1 ggaacatcat caattgatat tactggcagt ggagatctaa tatcattagg tggtcatgca
       61 ggacttggcg ttgcgtttgc taaaaagaaa ggtgaaatgt tatctgatat ttgtatttat
      121 gctacagcat gtggacatgc aggaattggt ggtgggataa atgcggctat cacatattct
      181 gagaccaagt ctttacctac atcgggagtc agtaattcag taggtgtaac ggttggcggc
      241 ggagttgggg ggcattttgc gtatacttat gtagtggatg ttgataatcc agaatcatcg
      301 acagaatctg ttggtatcgg tgcaggtgtt gacgcttcag ttatgactct ggcttgtaga
      361 acgtggcaag aatgctgggt caattaacag gaggaaatat gaaaaaatat atatttttag
      421 ttttattttt attgtgtg
//
LOCUS       DAZFLD010000778          431 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.781, whole genome shotgun sequence.
ACCESSION   DAZFLD010000778 DAZFLD010000000
VERSION     DAZFLD010000778.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..431
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.781"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            16..>431
                     /locus_tag="ACHKBL_005517"
     CDS             16..>431
                     /locus_tag="ACHKBL_005517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFO5822202.1"
                     /translation="MKRPNMPVQGQGFVQPEISQNDILIRHKAESRIDSRVISERAGI
                     QHESIIATIKSHQSRLRELGSLPRQSVKELTYLKSGKSGRKRGRPEISYLLNELQLDY
                     LLRVIRGRDAERINQFKLDVTKAFAKHRAAEPVRRE"
BASE COUNT          116 a          104 c          108 g          103 t
ORIGIN      
        1 agcaggagtt tttcaatgaa acgtccgaat atgcccgtac aggggcaggg cttcgttcag
       61 cctgaaatca gccagaacga tatattgatt cgccacaaag cggagtcgcg tattgattcc
      121 cgcgttattt ctgagcgagc aggaatacaa cacgaaagta tcattgcgac gattaaatct
      181 catcaatcca gattgcgcga gcttggctca ctgcctcgcc agtcagtcaa ggaattaacc
      241 tatttgaaat cgggaaaatc tggccgcaag cgcggtcgtc ctgaaattag ttatttgctg
      301 aatgagctac agcttgacta tctgttacgc gttatccgtg ggcgtgacgc agaacgtatc
      361 aaccagttca agctggatgt tacgaaggca ttcgcgaagc atcgcgccgc tgaaccagtc
      421 cgtcgcgaat a
//
LOCUS       DAZFLD010000779          430 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.782, whole genome shotgun sequence.
ACCESSION   DAZFLD010000779 DAZFLD010000000
VERSION     DAZFLD010000779.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..430
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.782"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>430)
                     /locus_tag="ACHKBL_005518"
     CDS             complement(<1..>430)
                     /locus_tag="ACHKBL_005518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000097914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ImpA family type VI secretion system protein"
                     /protein_id="HFO5822203.1"
                     /translation="SQEERLSLISRANEALSLWDNWLHPFTSGSETGDDPAYDDNFQL
                     MREEINKISGTDSALLCELAQKCLCECARDIRVVTWYVLARLTRDGERGLSEGLLLLV
                     AMLTRYGQACHPRRPAARKAALEWLNSAKVTDALLLWPGV"
BASE COUNT          101 a          128 c          109 g           92 t
ORIGIN      
        1 tccaccccgg gccataacag cagtgcatca gtcacttttg cactgttcag ccactccagc
       61 gcagctttcc gggcggcagg acgccgggga tgacatgcct gtccgtagcg ggtcagcatc
      121 gcaacaagaa gtaataagcc ttcggacaac cctctttcac catcccgggt gagacgggcc
      181 agcacgtacc aggtcacgac acgaatatct ctggcgcatt cgcaaaggca cttctgagcc
      241 agttcgcaca acagcgctga atcagtacct gaaatcttat tgatctcttc acgcatcagc
      301 tgaaaattat catcgtatgc aggatcgtcc ccggtttcag atccggaggt aaatggatgc
      361 agccagttat cccagagcga cagggcctca tttgcgcggg aaatgaggct cagcctttct
      421 tcctggctgg
//
LOCUS       DAZFLD010000780          429 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.783, whole genome shotgun sequence.
ACCESSION   DAZFLD010000780 DAZFLD010000000
VERSION     DAZFLD010000780.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..429
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.783"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(228..>429)
                     /locus_tag="ACHKBL_005519"
     CDS             complement(228..>429)
                     /locus_tag="ACHKBL_005519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ethanolamine utilization protein EutE"
                     /protein_id="HFO5822204.1"
                     /translation="LEIHTTYVSLPMIPLEISGISARTLANTMHTMAARGYEYKERFP
                     QMIRYRVKEWEKVAGVPVAEVF"
BASE COUNT          104 a          102 c          108 g          115 t
ORIGIN      
        1 gcgtttcagc atagcatgca gttctgtgct gattcttgct tccagtcggg cagtttgtgt
       61 tgcagatggc atgatttgta cctcctgaat acactgtacg gcaaattgcc gtattgtgca
      121 aattacagtc tgatacaggg acggcagaca ggcattctgc ctgaaaagaa aaagccggtt
      181 cagagaaaac cggctgacgg attatcgtct aacagttggt cgctacctta aaatacttct
      241 gcaacaggca caccggcaac tttttcccat tctttaaccc gataccgaat catctgcggg
      301 aaacgttcct tgtactcata tccacgtgca gccatggtgt gcatggtgtt ggccagcgtc
      361 cttgctgaga tgccggagat ttcaagaggg atcatcggca gactcacata ggtggtgtgg
      421 atttccagc
//
LOCUS       DAZFLD010000781          427 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.784, whole genome shotgun sequence.
ACCESSION   DAZFLD010000781 DAZFLD010000000
VERSION     DAZFLD010000781.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..427
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.784"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>427)
                     /locus_tag="ACHKBL_005520"
     CDS             complement(<1..>427)
                     /locus_tag="ACHKBL_005520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFO5822205.1"
                     /translation="QMNEGWYQIRIAGEDTAPEAVYACLHEQLGEGAVIHIVPRLAGA
                     GGNGVFQVVLGVAAIVGSFFTGGATLAAWGAALSAGGLTATTMLFSLGASMILGGVAQ
                     MLAPKAKTPEYKSTDNGKQNTYFSSLDNMIAQGNPMPVP"
BASE COUNT           91 a          146 c          101 g           89 t
ORIGIN      
        1 taaggcaccg gcatcgggtt accctgggca atcatgttgt ccagtgacga aaaatacgtg
       61 ttctgtttac cgttatccgt acttttgtac tccggcgtct ttgccttcgg ggccagcatc
      121 tgggccacac cacccagtat catgctggca cccagtgaga acagcatcgt ggtggcagtc
      181 agccctccgg cactcagggc tgcgccccat gccgccagtg ttgcaccacc agtgaaaaat
      241 gacccgacaa tcgctgcaac ccccagcacc acctgaaaaa caccatttcc cccggctccg
      301 gccagtcgcg gcacaatgtg gatgaccgct ccctcaccca gttgttcgtg aagacaggca
      361 tacaccgcct ccggcgcggt atcctcaccg gcaatacgta tctgatacca gccttcgttc
      421 atctgac
//
LOCUS       DAZFLD010000782          423 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.785, whole genome shotgun sequence.
ACCESSION   DAZFLD010000782 DAZFLD010000000
VERSION     DAZFLD010000782.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..423
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.785"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..266)
                     /locus_tag="ACHKBL_005521"
     CDS             complement(<1..266)
                     /locus_tag="ACHKBL_005521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001608786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HFO5822206.1"
                     /translation="MNIRKLFCPDNTPRILLFLFFFVISVITTIACGYTEKNATGNVL
                     LLFLLLLLAHRNTLTSITALLFLFCCALYAPAGMTYGKINNSFIV"
BASE COUNT          151 a           73 c           90 g          109 t
ORIGIN      
        1 acaataaaac tgttgttgat tttaccgtac gtcatacccg caggcgcata cagtgcacaa
       61 cagaacagaa ataacagagc tgtaatggat gtaagggtat ttctgtgtgc aaggagcaga
      121 agaaggaaca gcagcaacac attcccggtg gcattcttct cagtgtatcc gcatgcaatt
      181 gtggttatga cagaaataac aaaaaagaat aaaaacaata aaatccgtgg tgtgttgtcc
      241 ggacaaaaca gttttctgat attcatcgga gtatatcgac aacattattg tgaagagaat
      301 aaaaatcaga agtctcctgt gaaacagata acaaacaacc ctgcagtata attactgcag
      361 ggagtcttcg tttaattaca gaagatgggt aattattatc tttcaggagc agttttgtca
      421 gaa
//
LOCUS       DAZFLD010000783          423 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.786, whole genome shotgun sequence.
ACCESSION   DAZFLD010000783 DAZFLD010000000
VERSION     DAZFLD010000783.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..423
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.786"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>423
                     /locus_tag="ACHKBL_005522"
     CDS             <1..>423
                     /locus_tag="ACHKBL_005522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5822207.1"
                     /translation="SDPEFMFKFSYSAQQNRLEPHLLVPTYFPLKTNEPVTQEDVLLL
                     YRFFKMDGDFNILTSDEYMSIMTPLMKCERSIHDHNRYVTGKDTLLLDYPPSGNQIYF
                     HIFPDESASLVLYFSNNDIECFVFERDIPSQYRFFKMFT"
BASE COUNT          127 a           88 c           71 g          137 t
ORIGIN      
        1 gagcgatccg gaatttatgt tcaaatttag ttatagcgct caacaaaata gattagaacc
       61 tcatcttctg gttcccactt atttccctct aaaaacaaac gaaccggtta cgcaagagga
      121 tgtactgtta ctttatcgtt tctttaaaat ggatggtgat tttaatatat taacatcaga
      181 tgaatacatg agtatcatga cacctctgat gaagtgtgag cgcagcatcc acgaccacaa
      241 caggtatgtg acgggcaaag atacccttct tctggactat cctccttctg gtaaccagat
      301 atatttccat atttttcctg acgaatcagc atcactagtc ctttattttt caaacaatga
      361 cattgaatgt ttcgtctttg aaagagatat tccgagccag tatcgctttt tcaaaatgtt
      421 tac
//
LOCUS       DAZFLD010000784          416 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.787, whole genome shotgun sequence.
ACCESSION   DAZFLD010000784 DAZFLD010000000
VERSION     DAZFLD010000784.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..416
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.787"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(115..>416)
                     /locus_tag="ACHKBL_005523"
     CDS             complement(115..>416)
                     /locus_tag="ACHKBL_005523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5822208.1"
                     /translation="NELKGSWISGPTLTSGSSWNNACEENGVTFSLSEDNFSIDPVLG
                     YTGHAAPWLAITLADAAFEQRGAQVIAAQPTADKDDVWVAVITKEEVRKESPGNG"
BASE COUNT          102 a          106 c          111 g           97 t
ORIGIN      
        1 aagccggtgg ccgaagcgac gttttccgga aaggcataaa aaagaaatgc cacgatgacc
       61 gtcagaaaaa aggcgacagt cacaacagag ccgatgagtg ttttaacttt taccttagcc
      121 attcccgggt gattccttgc gaacttcttc tttagtgatg acggcaaccc agacgtcgtc
      181 tttgtcagct gtgggctggg cggcgataac ctgcgcacct cgttgttcaa aggcagcatc
      241 agcaagcgtg atggcaagcc atggagcagc atggccggta tagcccagca cgggatcgat
      301 actgaaattg tcttctgaaa ggctgaatgt cactccgttc tcttcgcagg cattattcca
      361 gctgctgccc gacgtcaatg tgggaccgga gatccagctt cctttaagct cattgg
//
LOCUS       DAZFLD010000785          416 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.788, whole genome shotgun sequence.
ACCESSION   DAZFLD010000785 DAZFLD010000000
VERSION     DAZFLD010000785.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..416
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.788"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..274)
                     /locus_tag="ACHKBL_005524"
     CDS             complement(<1..274)
                     /locus_tag="ACHKBL_005524"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016241930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L deacetylase family protein"
                     /protein_id="HFO5822209.1"
                     /translation="MDKVLDSAILSSANKRKGILAIGAHPDDIELGCGASLARLAQKG
                     IYIATVVMTTGNSGVDGIIDRHEESRNALKILGCHQTIHLNFADTRA"
BASE COUNT          120 a           93 c           92 g          111 t
ORIGIN      
        1 gagcgcgggt gtcagcaaaa ttaagatgaa tagtttggtg gcaccctaat atctttagag
       61 cgttgcgtga ttcttcgtgg cgatcgatta ttccatctac gccagaatta ccggtagtca
      121 tcaccacggt agcaatataa attccttttt gcgcaaggcg agctagcgat gcgccacagc
      181 ctaattctat atcgtcagga tgcgcgccaa tggcaaggat accctttctt ttatttgccg
      241 aagaaaggat cgctgaatct aaaaccttat ccactttatg acactccatt ttatttatta
      301 tactacaagc acaacgacgc ccgcagagac gtaacctctg acgcagacag atgctaattt
      361 ttaagtgtta aaaatagctg atagctgaaa aacaaggggc tgccaggtac ggggat
//
LOCUS       DAZFLD010000786          414 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.789, whole genome shotgun sequence.
ACCESSION   DAZFLD010000786 DAZFLD010000000
VERSION     DAZFLD010000786.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..414
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.789"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            182..>414
                     /locus_tag="ACHKBL_005525"
     CDS             182..>414
                     /locus_tag="ACHKBL_005525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P2 family phage major capsid protein"
                     /protein_id="HFO5822210.1"
                     /translation="MQLNQKTESLLRKYAAGLGAANGVDDVTRYFALSDPKETALRDA
                     IMHSDEFLSKLPNVLDVEQVTGQVVTTGVPGLHT"
BASE COUNT          107 a          127 c          109 g           71 t
ORIGIN      
        1 aacgttaccg ccgctttgcc gcccgtcgcc gggacgacac cgacaaagaa gaaatcaaag
       61 ccattgccga aaaacttaac gacctgacca cgaagctgtc agccaccggc atcacgccac
      121 gcccggctgg cacaccggca gaaagcgaaa aaccgtttga ctttgtttaa ggacacgcaa
      181 catgcaactg aaccagaaaa ccgaatcact gcttcgtaag tacgctgccg ggctgggcgc
      241 ggcaaacggg gttgacgacg tcacacgcta ttttgcgctg tctgacccga aggagaccgc
      301 gctgcgtgac gccatcatgc actccgacga gtttttatca aaactgccga acgtgctgga
      361 tgttgaacag gtgacaggcc aggtggttac caccggcgtt ccgggcctcc atac
//
LOCUS       DAZFLD010000787          413 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.790, whole genome shotgun sequence.
ACCESSION   DAZFLD010000787 DAZFLD010000000
VERSION     DAZFLD010000787.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..413
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.790"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..200)
                     /locus_tag="ACHKBL_005526"
     CDS             complement(<1..200)
                     /locus_tag="ACHKBL_005526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009309580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HFO5822211.1"
                     /translation="MDDLTLRYYEAEMRYLREAGKEFARAHPDRAAMLNLDKPGACDP
                     GVERLFEGFAFLMGRLREKLDD"
     gene            complement(234..>413)
                     /locus_tag="ACHKBL_005527"
     CDS             complement(234..>413)
                     /locus_tag="ACHKBL_005527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004173386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssA"
                     /protein_id="HFO5822212.1"
                     /translation="TLEDWEPALLFEVRARRLKLLRMKAGRSESDKARLMPEMDTLLA
                     GLIAIDPARAMVLCG"
BASE COUNT          101 a          130 c          101 g           81 t
ORIGIN      
        1 tcatcatcca gcttttcacg caaacgtccc atcaggaagg caaagccttc aaacagacgt
       61 tccacaccgg gatcgcaggc gccaggctta tccagattca gcattgctgc ccgatccgga
      121 tgagcacggg caaactcttt accggcttcc cgcagatagc gcatctctgc ttcgtaatag
      181 cgcagggtta aatcatccat gcacggcatt cctgaaaatg attaaagaga acatcagcca
      241 cagagcacca tggcacgggc cgggtcaatg gcgatcagcc ccgccagcag cgtatccatc
      301 tcaggcatca gacgggcttt atcagattcg ctgcgaccgg ctttcatccg tagcagcttc
      361 aggcgtcggg cccggacttc aaacagcaaa gcgggctccc agtcttccag cgt
//
LOCUS       DAZFLD010000788          412 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.791, whole genome shotgun sequence.
ACCESSION   DAZFLD010000788 DAZFLD010000000
VERSION     DAZFLD010000788.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..412
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.791"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(125..316)
                     /gene="yfgG"
                     /locus_tag="ACHKBL_005528"
     CDS             complement(125..316)
                     /gene="yfgG"
                     /locus_tag="ACHKBL_005528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfgG family protein"
                     /protein_id="HFO5822213.1"
                     /translation="MSQATSMRKRHRFNSRMTRIVLLISFIFFFGRFIYSSVGAWQHH
                     QSKKEAQQSTLSVESPVQR"
     gene            334..>412
                     /locus_tag="ACHKBL_005529"
     CDS             334..>412
                     /locus_tag="ACHKBL_005529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5822214.1"
                     /translation="MSHAVDGTYIKAQQDKMVNSSLCKKA"
BASE COUNT          125 a           79 c          107 g          101 t
ORIGIN      
        1 atcagtcagg acaaaaacgt aaaatttaag atgaaaaaat atctgccgct ttgataatcc
       61 caaagagcaa aagcatccga cagcgaggtg ccgttatgtt ctgtgagagt ggagaaggta
      121 accgctaacg ttgtactggt gattcgacgg agagtgtgga ttgctgagct tcttttttgc
      181 tctgatggtg ctgccaggca ccgacggacg agtagataaa acggccaaag aagaagataa
      241 agctgatgag cagtacgata cgggtcatgc gactgttaaa tcggtgtcgt tttcgcatac
      301 tggttgcctg actcacaaaa ggttccttga agtatgtccc acgccgtgga cggtacttac
      361 attaaggcac aacaggacaa aatggtcaat tcttcgttat gtaaaaaagc gt
//
LOCUS       DAZFLD010000789          410 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.792, whole genome shotgun sequence.
ACCESSION   DAZFLD010000789 DAZFLD010000000
VERSION     DAZFLD010000789.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..410
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.792"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>410)
                     /locus_tag="ACHKBL_005530"
     CDS             complement(<1..>410)
                     /locus_tag="ACHKBL_005530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HFO5822215.1"
                     /translation="NAGVTRDNLINNMTEQDWDIVIDINLKGVFNITQAVAPIMMEQN
                     HGAIITMSSVVGTDGNIGQSNYAATKGGVIAMSKGWAKEFSRKGAKVRVNCVAPGFVI
                     TPMTENLPEKVINHMIAKTPLGRMATPEDISNGV"
BASE COUNT          114 a           93 c           64 g          139 t
ORIGIN      
        1 aacaccattc gaaatatcct caggtgttgc cattcgccct aaaggggttt tagctatcat
       61 atggttaatg actttctctg gcagattttc tgtcatcggg gtaataacga atcctggagc
      121 tacacaattc acgcgtactt tagcaccttt tcgggaaaat tctttagccc aacctttaga
      181 cattgcaata acaccacctt tagttgcggc ataattactc tgacctatat taccatcagt
      241 tcctacaaca gaagacattg ttatgattgc cccatgattt tgttccatca taataggtgc
      301 aactgcctga gttatattaa aaacaccttt aaggtttata tcaattacaa tatcccagtc
      361 ttgctctgtc atattattga tcagattatc tcttgtaact cctgcgttat
//
LOCUS       DAZFLD010000790          410 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.793, whole genome shotgun sequence.
ACCESSION   DAZFLD010000790 DAZFLD010000000
VERSION     DAZFLD010000790.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..410
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.793"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>410)
                     /locus_tag="ACHKBL_005531"
     CDS             complement(<1..>410)
                     /locus_tag="ACHKBL_005531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004115529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer ATPase"
                     /protein_id="HFO5822216.1"
                     /translation="KLDRTQNAHLLLFGPTGAGKSATLCGSLSQIMAVHRPRLFIAEA
                     GNSFGLLADYFESLGLSVNKISVKPGTGVCLPPFADAHQLVEQGETLQSVDEHSLPDL
                     DEDEGDEEEEKRDILGEMEISARMMITGGDPKEEA"
BASE COUNT           95 a          133 c           89 g           93 t
ORIGIN      
        1 gcttcttctt tcggatcccc tccggtgatc atcatgcggg cagagatttc catctccccc
       61 agaatgtcgc gtttctcttc ctcttcatcc ccctcatctt catcaagatc gggcagtgaa
      121 tgctcatcca cactctgcag cgtctctccc tgctccacca gttgatgagc atccgcaaat
      181 ggaggaagac acacccccgt tcccggtttg acactgattt tattaaccga aagcccgaga
      241 ctctcaaaat aatccgccag cagaccaaac gagttcccgg cctcagcgat aaacagacgg
      301 ggacgatgaa ctgccattat ctgcgacaac gaaccacaca gcgtggccga tttaccggct
      361 ccggtaggcc cgaaaagcag cagatgtgca ttctgggtcc ggtccagctt
//
LOCUS       DAZFLD010000791          407 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.794, whole genome shotgun sequence.
ACCESSION   DAZFLD010000791 DAZFLD010000000
VERSION     DAZFLD010000791.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..407
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.794"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>407
                     /locus_tag="ACHKBL_005532"
     CDS             <1..>407
                     /locus_tag="ACHKBL_005532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5822217.1"
                     /translation="HQIFKNQLDTETITVSYEFILCQHLITNTEFSYLALPENYNRLF
                     LPNSKNKTNNGLKTLNSEAVGRLLAAGGVYNGNIEGFRDTAEKLGGDAIKGYDQILNE
                     KTAGIAIATASILLTKRSNVDTYTEINSYLGKL"
BASE COUNT          152 a           75 c           73 g          107 t
ORIGIN      
        1 caccagatct ttaaaaacca actggataca gaaacaataa ctgttagtta tgaatttatc
       61 ctctgccaac atcttatcac taacaccgag ttcagctatt tggcactccc tgaaaattat
      121 aaccgcttgt ttttaccaaa ttcaaaaaac aaaacaaata atggtttaaa aacgttaaac
      181 agcgaagctg ttggcaggct acttgctgct ggtggcgtat acaatgggaa cattgaaggt
      241 ttcagagata ctgcggaaaa actgggtgga gatgcaatca aaggctatga tcaaatacta
      301 aatgaaaaaa cagcgggcat agcgatagca acagcatcta ttcttttaac aaagcgttct
      361 aatgttgata catatacaga aataaatagt tacttaggca aacttag
//
LOCUS       DAZFLD010000792          407 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.795, whole genome shotgun sequence.
ACCESSION   DAZFLD010000792 DAZFLD010000000
VERSION     DAZFLD010000792.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..407
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.795"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..384
                     /gene="fimB"
                     /locus_tag="ACHKBL_005533"
     CDS             <1..384
                     /gene="fimB"
                     /locus_tag="ACHKBL_005533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimB"
                     /protein_id="HFO5822218.1"
                     /translation="GFSTTHPLLNKEIQALKNWLSIRTSYPHAESEWVFLSRKGNPLS
                     RQQFYHIISTSGGNAGLSLEIHPHMLRHSCGFALANMGIDTRLIQDYLGHRNIRHTVW
                     YTASNAGRFYGIWDRARGRQRHAVL"
BASE COUNT          101 a           86 c          103 g          117 t
ORIGIN      
        1 ggcttttcaa caacgcaccc gctattgaac aaagaaattc aggctttaaa aaactggttg
       61 agtatccgta cttcttatcc gcatgctgag agcgagtggg tattcttatc acgtaagggg
      121 aatccacttt ctcggcaaca gttttaccat attatctcga cttccggtgg taatgccggg
      181 ttgtcactgg agattcatcc gcacatgtta cgccattcgt gtggttttgc tttggcgaat
      241 atgggaatag atacgcgact tatccaggat tatcttgggc atcgcaatat tcgtcatact
      301 gtctggtata ccgccagcaa tgcagggcgt ttttacggca tctgggatag agccagaggg
      361 agacagcgtc acgctgtttt atagagatag ttgagatacc agggatg
//
LOCUS       DAZFLD010000793          405 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.796, whole genome shotgun sequence.
ACCESSION   DAZFLD010000793 DAZFLD010000000
VERSION     DAZFLD010000793.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..405
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.796"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
BASE COUNT          115 a           85 c           71 g          134 t
ORIGIN      
        1 gatcaggtat cagtttgcaa aaatgtttgt tataggtcat gaccgcaacc aggacacata
       61 atttcaccac aaccacaaat ataatcggca tcatagtcat gtgttactat accggaagcc
      121 gacttattat ttgtgataca gtgtgtctcc tgctctacgg aggattctac ctctatagga
      181 aaattatcgt gttttcgtct tgaattgtct tgcttgttgc ctttatcaaa ggtaacaatg
      241 aattcttcat ttctgtgatt attatcacaa ttcattctta tgcctcctca catttcccga
      301 tgtatgaccg tcagtgctat tagtgtgcgt tcaagtctca ggttatacat gagcgacaac
      361 catgagatcc tgactaacaa ttgcatcagt gacataacgt aaaat
//
LOCUS       DAZFLD010000794          405 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.797, whole genome shotgun sequence.
ACCESSION   DAZFLD010000794 DAZFLD010000000
VERSION     DAZFLD010000794.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..405
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.797"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>405
                     /locus_tag="ACHKBL_005534"
     CDS             <1..>405
                     /locus_tag="ACHKBL_005534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CHASE9 sensor domain-containing protein"
                     /protein_id="HFO5822219.1"
                     /translation="LDSVGLVLDDNKYYLFSRRANDKIVVYHQEQVNGPLVDESGRVI
                     FADFNPSKRPWSMASDDSNNSWNPAYNCFDRPGKKCISFTLRINGKDHDLLVVDKIHV
                     DLNWRYLNEYLDHISANDEVLFLKQGHEIIAK"
BASE COUNT          118 a           74 c           93 g          120 t
ORIGIN      
        1 tttagactcg gtgggactgg tacttgatga taataaatat tatctctttt cgcggagggc
       61 gaatgataaa atcgttgttt atcatcagga acaagtaaat ggaccgcttg tcgacgagtc
      121 agggcgggtt atttttgccg attttaaccc atcgaaacga ccgtggtcga tggcttcaga
      181 tgactctaac aacagctgga atccggcata caattgcttt gatcgtccgg gtaaaaaatg
      241 tatctctttt acgctacgca tcaacggcaa agatcacgat ttgttagtgg tggataaaat
      301 tcatgtcgat ttaaactggc gatatctgaa cgagtatctt gatcacatca gcgctaatga
      361 tgaagttcta tttttaaagc aaggtcatga gatcattgcc aagaa
//
LOCUS       DAZFLD010000795          404 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.798, whole genome shotgun sequence.
ACCESSION   DAZFLD010000795 DAZFLD010000000
VERSION     DAZFLD010000795.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..404
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.798"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(93..302)
                     /gene="fumD"
                     /locus_tag="ACHKBL_005535"
     CDS             complement(93..302)
                     /gene="fumD"
                     /locus_tag="ACHKBL_005535"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase FumD"
                     /protein_id="HFO5822220.1"
                     /translation="MGNRTKEDELYREMCRVVGKVVLEMRDLGQEPKHIVIAGVLRTA
                     LANKRIQRSELEKQAMETVINALVK"
BASE COUNT          119 a           98 c           61 g          126 t
ORIGIN      
        1 tataatttca gcagttgtta tttagaatga ttaactggac cgcaactgaa gcaagaatat
       61 caggatgaag cttaccgcct catcctgaat ggtcatttta ccagtgcgtt aatgacggtt
      121 tccatcgcct gcttttccag ctcactgcgc tgaatgcgtt tattggctaa tgctgtacgt
      181 aatacgcctg cgataacaat atgctttggt tcctgcccca gatcgcgcat ttccagcaca
      241 actttaccga caactctgca catttctcgg tacagttcat cctcttttgt ccgatttccc
      301 attctttata cctatttatc atgccaacta tcagcatata tcaatctaac caattaaaca
      361 aaaatcaaac aaaatcagac aaataacgcg ataaattatt ttaa
//
LOCUS       DAZFLD010000796          404 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.799, whole genome shotgun sequence.
ACCESSION   DAZFLD010000796 DAZFLD010000000
VERSION     DAZFLD010000796.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..404
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.799"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>404
                     /locus_tag="ACHKBL_005536"
     CDS             <1..>404
                     /locus_tag="ACHKBL_005536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HFO5822221.1"
                     /translation="FGVTYTGNYEFPQPLHDALDKFQADTGIDIDMHIDAASGGFLAP
                     FVAPDIVWDFRLPRVKSISASGHKFGLAPLGCGWVIWRDEEALPQELVFNVDYLGGQI
                     GTFAINFSRPAGQVIAQYYEFLRLGREGYTKV"
BASE COUNT           76 a          121 c          115 g           92 t
ORIGIN      
        1 ttcggcgtga cctacaccgg taactatgag ttcccgcagc cgctgcacga tgcgctggat
       61 aaattccagg ccgacaccgg tatcgacatc gacatgcaca tcgacgccgc cagcggtggc
      121 ttcctggcac cgttcgtcgc cccggatatc gtctgggact tccgcctgcc gcgtgtgaaa
      181 tcgatcagtg cttcaggcca taaattcggt ctggctccgc tgggctgcgg ctgggttatc
      241 tggcgtgatg aagaagcgct gccgcaggaa ctggtgttca acgttgacta cctcggcggt
      301 cagattggta ctttcgccat caacttctcc cgcccggcgg gtcaggtgat tgcacagtac
      361 tatgaattcc tgcgcctcgg tcgtgaaggc tataccaaag taca
//
LOCUS       DAZFLD010000797          402 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.800, whole genome shotgun sequence.
ACCESSION   DAZFLD010000797 DAZFLD010000000
VERSION     DAZFLD010000797.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..402
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.800"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..320)
                     /locus_tag="ACHKBL_005537"
     CDS             complement(<1..320)
                     /locus_tag="ACHKBL_005537"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HFO5822222.1"
                     /translation="MLKRYLVLSVVTAAFSLPSLVYAAQQNILSVHILNQQTGKPAAD
                     VTVTLEKKADNGWLQLNTAKTDKDGRIKALWPEQTATTGDYRVVFKTGDYFKKQNLES
                     FFPE"
     gene            342..>402
                     /locus_tag="ACHKBL_005538"
     CDS             342..>402
                     /locus_tag="ACHKBL_005538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310734.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5822223.1"
                     /translation="MSFEDSEKASRVTLQQHYNF"
BASE COUNT          105 a           80 c           91 g          126 t
ORIGIN      
        1 atctcaggga agaaactttc aagattttgt ttcttgaaat agtccccggt tttaaatacg
       61 acacggtaat cgcccgtagt cgcagtttgc tcgggccaca gtgccttaat tcgtccatcc
      121 ttatctgttt tggcggtatt aagttgtaac cagccgttat ccgctttctt ttcaagagtg
      181 actgtcacgt cggcagcggg ttttcctgtt tgctggttca aaatgtgcac gctaagaatg
      241 ttttgttgtg cagcatagac caaagaaggt aatgaaaatg cagccgttac tacggagagt
      301 actaaataac gctttaacat gtttatatcc ttgtcatgtg aatgagtttt gaggatagcg
      361 aaaaagcgtc cagggtaacc ttacagcagc attacaattt tg
//
LOCUS       DAZFLD010000798          402 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.801, whole genome shotgun sequence.
ACCESSION   DAZFLD010000798 DAZFLD010000000
VERSION     DAZFLD010000798.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..402
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.801"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..250
                     /locus_tag="ACHKBL_005539"
     CDS             <1..250
                     /locus_tag="ACHKBL_005539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000282231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFO5822224.1"
                     /translation="EFIELLGEFTNPTGDNAQLKGEELDKYWDDLEEHLTRITQHPLM
                     SDLIYYPAKKGDDKPENILKIVKEWRRSQGLSLFKDSE"
BASE COUNT          137 a           73 c           86 g          106 t
ORIGIN      
        1 ggaattcatt gagttgcttg gcgagtttac aaatccaacc ggggataacg ctcaattaaa
       61 aggggaagag cttgacaaat attgggatga tctagaagaa catcttacca gaattacaca
      121 acatccgttg atgagcgatc ttatctacta tcctgcaaag aagggagatg ataagcctga
      181 aaacatcctg aaaatcgtca aagaatggcg gcgttcgcaa ggattgtctt tgttcaaaga
      241 ttcagaatga attctaaaca gaatctgtca cctgaaaatc atcacgttct ggttaagtga
      301 tgattttcgc tacgagaagg ttacggtggc gattgcagtg ataataagca atgtaaaaag
      361 aaaacatagg taaatactac ctttctctcc ttccctgaac at
//
LOCUS       DAZFLD010000799          399 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.802, whole genome shotgun sequence.
ACCESSION   DAZFLD010000799 DAZFLD010000000
VERSION     DAZFLD010000799.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..399
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.802"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>399
                     /locus_tag="ACHKBL_005540"
     CDS             <1..>399
                     /locus_tag="ACHKBL_005540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000367546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HFO5822225.1"
                     /translation="IGNIHEYILAYAKNNEISKEFLMRKDGIDDVYQFIEKLKKEKVP
                     LDKAEQQLKKFYSSNGYDRGITLYNGLNEDYRPWGKINMSWPNADSFGPTYEVLHPLT
                     NNPVKIPDRGWRWKEGTFNHIAKRIDGKYAD"
BASE COUNT          147 a           59 c           86 g          107 t
ORIGIN      
        1 attggtaata tccatgagta catccttgct tatgcgaaga ataacgaaat aagtaaagaa
       61 tttttaatgc ggaaagatgg tattgatgat gtatatcagt ttattgaaaa gttaaaaaag
      121 gaaaaggtgc cattagataa agcagagcag caactcaaaa aattctacag ctccaacgga
      181 tatgatcgtg gaattactct ttacaacggt ttgaacgagg attataggcc atggggtaaa
      241 attaatatga gttggcctaa tgcagattca tttggaccca cgtatgaagt attgcaccct
      301 ttgactaata accctgtcaa aatcccagac agaggctggc gttggaaaga aggcactttt
      361 aatcatattg caaaaagaat agatggaaag tatgcagat
//
LOCUS       DAZFLD010000800          397 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.803, whole genome shotgun sequence.
ACCESSION   DAZFLD010000800 DAZFLD010000000
VERSION     DAZFLD010000800.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..397
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.803"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            244..>397
                     /locus_tag="ACHKBL_005541"
     CDS             244..>397
                     /locus_tag="ACHKBL_005541"
                     /inference="COORDINATES: protein motif:HMM:NF023154.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HFO5822226.1"
                     /translation="MPQDDFCIVNPGGCIIHFPANGRYILDIHDPDFPEQYQRLLERL
                     DYLTKPG"
BASE COUNT           87 a          103 c           98 g          109 t
ORIGIN      
        1 aaatatccgc tctgcgcagg gcgttcagag gtattgccag cctgcatttt atccggtgtt
       61 tcgcacctac cccgttcgtg acacaaaggc gcgacaccgg atatccgcag tcctgcttac
      121 ctgaataaaa cccggggtta tgattgtgtg ctgtggaacg aaattgcacc gtgatcgcgg
      181 attcgccgaa tctgcactgt ggtgtcaata aaccacacgc gagagtcctc tccgtattga
      241 tcagtgcctc aggacgattt ctgcattgtt aatccagggg gctgcattat tcatttccct
      301 gctaatggac gttacatcct ggatattcat gatcctgatt tccctgagca gtatcagcgc
      361 ttattggagc gtctggatta tctgacgaag cccggta
//
LOCUS       DAZFLD010000801          397 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.804, whole genome shotgun sequence.
ACCESSION   DAZFLD010000801 DAZFLD010000000
VERSION     DAZFLD010000801.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..397
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.804"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>397
                     /locus_tag="ACHKBL_005542"
     CDS             <1..>397
                     /locus_tag="ACHKBL_005542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004173387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ImcF-related family protein"
                     /protein_id="HFO5822227.1"
                     /translation="LMLARPDKADAGWLAKNALKALPKRRNVPDGIWQNVAPKLLGFY
                     AQNLPAHPEWKIKPDDELISTVRQILLKQIGQRNAESGLYQEMLKRVASNWPDLTLAD
                     MTGDTDASTVFSTDEVVPGMFTRQAWEEQV"
BASE COUNT          101 a           96 c          117 g           83 t
ORIGIN      
        1 atctgatgct ggcgcgcccg gacaaagcgg acgccggctg gctggcaaaa aatgcgctga
       61 aagccttacc gaaacgccgg aatgtgccgg atggcatatg gcagaacgtc gcaccgaaac
      121 tgctggggtt ttatgcgcaa aaccttccgg ctcatccgga atggaaaatt aagcctgacg
      181 atgaattaat cagtacggtc cgtcagattc tgcttaagca aattggccag cggaatgctg
      241 agtccgggtt atatcaggag atgcttaagc gcgtggccag taactggcct gatttaacac
      301 ttgcggatat gaccggtgac accgatgcct caacggtatt cagcactgac gaagtcgttc
      361 cgggaatgtt tacgcgtcag gcctgggaag agcaggt
//
LOCUS       DAZFLD010000802          397 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.805, whole genome shotgun sequence.
ACCESSION   DAZFLD010000802 DAZFLD010000000
VERSION     DAZFLD010000802.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..397
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.805"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..267
                     /locus_tag="ACHKBL_005543"
     CDS             <1..267
                     /locus_tag="ACHKBL_005543"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007710822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HFO5822228.1"
                     /translation="GYRLTFGEAYRTPEQAALNAKSGKGIRNSLHTLRLAVDFNLFIN
                     GKYQADTDAYRPLGEYWESIGGTWGGRFSRADGNHFSLEHNGMK"
BASE COUNT           86 a          116 c          131 g           64 t
ORIGIN      
        1 ggctatcgcc tgaccttcgg cgaggcatac cgcacgccgg agcaggccgc actgaatgcg
       61 aaaagcggaa aaggcattcg taacagcctt cacacgctgc gcctcgcggt ggacttcaac
      121 ctgttcatta acggcaaata ccaggccgac accgacgcct acaggccgct gggggaatac
      181 tgggaatcca tcggcggcac atggggcggg cgcttcagtc gggcggacgg caatcatttc
      241 agccttgagc acaacgggat gaaatgatga aacagctggc gggctgcctg gtgatcctct
      301 ttgccgccat gtgggcaggc tggctggccc atgactggca tgacgccagg gtggcgcttg
      361 ccgaacagga agccatggaa gccacgcgac gggcggt
//
LOCUS       DAZFLD010000803          396 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.806, whole genome shotgun sequence.
ACCESSION   DAZFLD010000803 DAZFLD010000000
VERSION     DAZFLD010000803.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..396
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.806"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>396)
                     /locus_tag="ACHKBL_005544"
     CDS             complement(<1..>396)
                     /locus_tag="ACHKBL_005544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5822229.1"
                     /translation="LPLHLAALEGDENAFFSAEVNMVRRELQSVPGNSVNCMCWASLA
                     ALAFDAEERVERQKGNNENIATYDESIATYAFRAACTVSRCLSRNASGGIPRSYPEWK
                     AILFPRDGADIFPDGWLENMTRRAERVFTS"
BASE COUNT           98 a          120 c           93 g           85 t
ORIGIN      
        1 actcgtaaaa acacgttcag cccgacgggt catattttcc agccacccgt cggggaaaat
       61 gtcagcacca tcacggggaa acaaaatcgc tttccattca gggtatgagc gcggaatacc
      121 tccggaagca ttccggctca gacagcgact cacagtgcag gcagcacgga atgcatatgt
      181 tgcgatgctc tcatcatatg ttgcgatgtt ctcattattg cctttctgcc gttccacccg
      241 ctcttcagca tcaaatgcca gtgcagcaag gcttgcccag cacatgcagt tcactgaatt
      301 ccccggaacg ctctgcagct cccggcgcac catgttgacc tcagcactga aaaaagcgtt
      361 ctcatcgcct tcaagggcag ccagatgcag cggaag
//
LOCUS       DAZFLD010000804          395 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.807, whole genome shotgun sequence.
ACCESSION   DAZFLD010000804 DAZFLD010000000
VERSION     DAZFLD010000804.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..395
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.807"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..202
                     /locus_tag="ACHKBL_005545"
     CDS             <1..202
                     /locus_tag="ACHKBL_005545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5822230.1"
                     /translation="KEKKALEALKRVKTDTTNPLSPKTLGANAHAGFYPDPTALKVHW
                     DNISHILLFMLNKIFNSSSVHK"
BASE COUNT          131 a           86 c           76 g          102 t
ORIGIN      
        1 aaaagaaaag aaagctctgg aggctctgaa aagggttaaa actgatacga ccaatccact
       61 atctccaaaa acgctaggag caaatgccca tgcaggcttc tacccagatc caacggcgtt
      121 aaaagtccac tgggataaca tttcacatat cttattgttt atgttgaata aaatattcaa
      181 cagtagctct gtccataagt aatcaactgt tctaaaataa atcggtatga atctattttc
      241 acagggaaag actatgtcag caactccatc accattacga tatccgggag ggaaagccgc
      301 aattcaagat atgatttcgg ctataatttg ctccaacaac cttactggcg gacactatgc
      361 agaaccctac gctggaggtg gtggtcttgc tttat
//
LOCUS       DAZFLD010000805          395 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.808, whole genome shotgun sequence.
ACCESSION   DAZFLD010000805 DAZFLD010000000
VERSION     DAZFLD010000805.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..395
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.808"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>395
                     /locus_tag="ACHKBL_005546"
     CDS             <1..>395
                     /locus_tag="ACHKBL_005546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001608786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFO5822231.1"
                     /translation="IFWRTHHRGHRNWLALLLFVLCSVNSWPLRMVKGTVVGTTDTLR
                     EMQRYEQLSQHRADSWKILPGAPLYDTIVIVTGESVRRDYMSVYGYPVPTTPWLNTAP
                     GLFIDGYTSAAASTVPSLSRTLIYDYEQNP"
BASE COUNT           93 a           97 c          108 g           97 t
ORIGIN      
        1 attttctggc ggacacacca ccgcggtcac cgtaactggc tggcactgct gctgttcgta
       61 ttatgctctg taaacagctg gccgttgcgg atggttaaag gaaccgttgt ggggacaact
      121 gacacattgc gtgaaatgca gcgttatgaa caactgagtc agcacagggc tgacagctgg
      181 aaaatcctgc cgggagcacc gttgtatgac acgattgtga ttgtcactgg tgagagtgtg
      241 cgcagggatt atatgtcggt atatggttat cccgtaccaa ccacaccgtg gctgaatacg
      301 gctcccggtt tatttattga cggctataca tcggcagcag ccagtaccgt accttccctg
      361 agccggacac tgatttatga ctatgagcag aaccc
//
LOCUS       DAZFLD010000806          394 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.809, whole genome shotgun sequence.
ACCESSION   DAZFLD010000806 DAZFLD010000000
VERSION     DAZFLD010000806.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..394
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.809"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>394
                     /locus_tag="ACHKBL_005547"
     CDS             <1..>394
                     /locus_tag="ACHKBL_005547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5822232.1"
                     /translation="EKQPQIPEGASGMSPERRKSRFPGVLILSVTVGLVMGAVVYGVL
                     MLMTGLDSRITTLEGQRREMLSASELAPLRMKLTETEKQLKDMEKRLAVLEQQPVLTA
                     SWKEQLENLPEMLEQIESAREKLSLRLDT"
BASE COUNT          100 a           90 c          129 g           75 t
ORIGIN      
        1 gtgagaaaca gccgcagata ccggagggtg cgtctggtat gtcgccggag cgaagaaaaa
       61 gtcgtttccc cggcgtgttg atactgtctg tcactgtggg gctggtcatg ggggcagtgg
      121 tatatggcgt actgatgctt atgaccggtc ttgacagccg gatcacgacc ctggaaggcc
      181 agagaagaga aatgctttct gcgtcagaac tcgcgcccct gagaatgaaa ctgacagaga
      241 cggagaagca actgaaggat atggaaaagc gcctggcagt cctggagcag cagcccgtgc
      301 tgacagcatc ctggaaggaa caactggaga atctgccgga gatgctggag cagattgagt
      361 ctgcccggga aaagctttcc ctgagactgg atac
//
LOCUS       DAZFLD010000807          391 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.810, whole genome shotgun sequence.
ACCESSION   DAZFLD010000807 DAZFLD010000000
VERSION     DAZFLD010000807.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..391
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.810"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            34..273
                     /locus_tag="ACHKBL_005548"
     CDS             34..273
                     /locus_tag="ACHKBL_005548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO5822233.1"
                     /translation="MTLDEYLRQSSIRQADFAAIVGVSQGFVSGVITGRYMPQGRKAI
                     EWAKATDWNVTPHELNPHDYPNMEDGIPLHVRQTA"
BASE COUNT          116 a           85 c          102 g           88 t
ORIGIN      
        1 ctaatatcgg aaaattgaaa tgggaggatc tctatgactc ttgacgagta cttgagacaa
       61 agctctatca gacaagccga ttttgcggcg attgttggag tttctcaggg atttgtcagt
      121 ggggttatta cagggagata catgcctcag gggcggaaag ccattgaatg ggcaaaagct
      181 acagactgga atgtaactcc ccatgaacta aacccgcatg actacccaaa catggaagac
      241 ggtattccgc ttcatgtaag gcaaacagcc tgataaccct ccatcggagg tgtgagtgaa
      301 caggcaaatc aaaacggtta tgcccgatca gtactgtgat gctgatcggg actggataca
      361 ggagcaattg ttacagctag acccggccac c
//
LOCUS       DAZFLD010000808          389 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.811, whole genome shotgun sequence.
ACCESSION   DAZFLD010000808 DAZFLD010000000
VERSION     DAZFLD010000808.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..389
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.811"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>389)
                     /locus_tag="ACHKBL_005549"
     CDS             complement(<1..>389)
                     /locus_tag="ACHKBL_005549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5822234.1"
                     /translation="PPNAINWLAGKMAGSHASGDVTIIMIAENTHDLFMQSMAALTAV
                     LPVPVFTQAQRMAQAAASLSTDKMQIPFTTDTLPAPVHLSVATMRSAVSAARAAQARQ
                     LAAQRPDAAGLRRQISRFISRRAGALAG"
BASE COUNT           65 a          104 c          135 g           85 t
ORIGIN      
        1 ccggcaagtg cgccagcccg tctgctgata aagcggctta tctggcggcg aagccctgcg
       61 gcatccgggc gttgtgctgc cagctgcctg gcctgtgctg cgcgggcggc actgactgcg
      121 ctgcgcattg tggcaacgga aagatgcaca ggtgcgggca gggtgtcggt ggtgaacggg
      181 atttgcattt tgtcagtgct cagactggcg gcagcctgtg ccatgcgctg tgcctgcgtg
      241 aataccggta cgggcagaac ggcagtcagt gccgccattg actgcataaa aagatcatgg
      301 gtgttttcgg caatcatgat aatggtcacg tcgccggatg catggcttcc ggccattttc
      361 ccggccagcc agtttatggc gttcggggg
//
LOCUS       DAZFLD010000809          387 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.812, whole genome shotgun sequence.
ACCESSION   DAZFLD010000809 DAZFLD010000000
VERSION     DAZFLD010000809.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..387
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.812"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
BASE COUNT           86 a          102 c           96 g          103 t
ORIGIN      
        1 aaatgcccga cctgctcagg gcgttcagag gtatcggaac ctgcatttta tctggtgttt
       61 cgcacccacc ccgttcgtga cacaaaggcg cgacaccgga tatccgcagt cctgcttacc
      121 tgaataaaac ccggggtatt gattgtgtac tgtggtgcga aatcgcaccg tgatcgcgga
      181 ttcgccgaat ccgcactgtg gtgtcaacca accatacgcg agagtcctct ccgtatttat
      241 cagtgcctca ggacgatttc tgcatagtta atccaggggg ctgcattgtt tatttccctg
      301 ctaatggacg tgacatccag gatattcatg atcctgattt ccctgagcag tatcagcgct
      361 tattggagcg tctggattat cagacga
//
LOCUS       DAZFLD010000810          386 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.813, whole genome shotgun sequence.
ACCESSION   DAZFLD010000810 DAZFLD010000000
VERSION     DAZFLD010000810.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..386
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.813"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..195)
                     /locus_tag="ACHKBL_005550"
     CDS             complement(<1..195)
                     /locus_tag="ACHKBL_005550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000675383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5822235.1"
                     /translation="MKGAFTERIQANAPAWAHEQLVAWDTAEFAAMSDFITEHYWTGQ
                     GSINVYRIVGTDHPQYAGMSW"
     gene            complement(192..>386)
                     /locus_tag="ACHKBL_005551"
     CDS             complement(192..>386)
                     /locus_tag="ACHKBL_005551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000606112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5822236.1"
                     /translation="DIRELKMQAVVLLLGALFMEGVSSVILFRGCGSAAVSGEGRGGP
                     AVMPWPDGIGISKVQERRAE"
BASE COUNT           93 a          131 c           77 g           85 t
ORIGIN      
        1 ccagctcatt ccggcatact gtggatggtc agtccccaca atgcggtaca cattgatact
       61 gccctgtccc gtccagtagt gttccgtaat aaaatccgac attgccgcaa actctgcggt
      121 atcccatgca accagttgtt catgtgccca ggccggagca ttcgcctgta tccgctctgt
      181 aaatgccccc ttcattcagc tcttctctcc tgcacttttg agataccgat accgtcaggc
      241 cacggcataa cagccgggcc ccccctgcct tccccggata cggctgcaga accacaacca
      301 cgaaacagaa tgactgacga gacgccttcc ataaacaaag cgcccagcaa cagaaccact
      361 gcctgcattt tcagttcacg aatatc
//
LOCUS       DAZFLD010000811          385 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.814, whole genome shotgun sequence.
ACCESSION   DAZFLD010000811 DAZFLD010000000
VERSION     DAZFLD010000811.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..385
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.814"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>385
                     /locus_tag="ACHKBL_005552"
     CDS             <1..>385
                     /locus_tag="ACHKBL_005552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012311869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008754 - O antigen ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HFO5822237.1"
                     /translation="DNTRTSVGARLAMYEVGLKTYSPIGQSLEKRAEKIHELEEKEPR
                     LSGALPFVDSHLHNDLIDTLSTRGIPGVALTILAFSAIFIYALRTAKEPYILILLFSL
                     LVVGLSDVILFSKPVPTAVFVTIILL"
BASE COUNT          117 a           74 c           70 g          124 t
ORIGIN      
        1 gataacacca gaacttcagt cggtgcccgt ctggcaatgt atgaagttgg tttaaaaaca
       61 tattctccaa taggacaatc actggaaaaa cgtgcggaaa aaatacatga actagaagag
      121 aaagagccta gattaagtgg cgctttaccc tttgtagatt ctcatttgca taacgatctc
      181 atagatacgt tatcaacgcg tggtattcct ggagtagcat taacaatttt agccttttca
      241 gcaatattca tatatgcctt aagaactgct aaagaacctt atattttaat cttgcttttt
      301 tcactactgg tagtaggcct aagtgatgta atactctttt ctaaaccggt tccgactgct
      361 gtgtttgtca ccataatatt gcttt
//
LOCUS       DAZFLD010000812          379 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.815, whole genome shotgun sequence.
ACCESSION   DAZFLD010000812 DAZFLD010000000
VERSION     DAZFLD010000812.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..379
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.815"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
BASE COUNT           90 a          109 c          116 g           64 t
ORIGIN      
        1 attaatgata ctgccgtact ggaaaaggca gacgcatacc tggcacaggc ggcagcactg
       61 agccgtaccg ctggcgtaaa aagtatcagg gcgcgcatca gggcacgcat cagcgccctt
      121 aatgcagcgt aaacgactac ccgcagggcc gggcgggcgc gggaggaggc atcagtgacg
      181 gaaactgtat ggccgtggaa tcccggacag cccgcctttt tattcatatg aggcacgtca
      241 tgagcgaacc gagcttcagc gtcagcggta aaccgctgaa atccacaccg cagacggtga
      301 caaacggcga gccgttctgg ccggaccttg atctggctga gcttcagggg tcacgcacgc
      361 ttccggctga tttacccga
//
LOCUS       DAZFLD010000813          379 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.816, whole genome shotgun sequence.
ACCESSION   DAZFLD010000813 DAZFLD010000000
VERSION     DAZFLD010000813.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..379
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.816"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..290)
                     /locus_tag="ACHKBL_005553"
     CDS             complement(<1..290)
                     /locus_tag="ACHKBL_005553"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO5822238.1"
                     /translation="MEGLRAELTEDIPLTTPDFPSRIWLTVWWTGTLTSEWTVEHDII
                     MADPAATEEILLEAHRKEAARQYFFALADIDEDGNVTDLRPRGTPEERRWLDA"
BASE COUNT           70 a          116 c          102 g           91 t
ORIGIN      
        1 gcgtccagcc agcggcgttc ttccggcgtt cctctggggc gcaggtcagt cacgttgccg
       61 tcttcatcaa tgtcagcaag ggcaaaaaaa tattgccggg cggcctcctt cctgtgtgct
      121 tccagcagga tctcttccgt ggcggcgggg tcggccatga taatatcgtg ctccaccgtc
      181 cattcactgg tgagcgtacc ggtccaccat actgtcagcc agatacggga cgggaagtct
      241 ggcgtggtca gcggtatgtc ttcggtcagc tctgcgcgta atccctccac gtaaccggtc
      301 cctttgcaga gtgaccatct ttcgcctttt ttaaccagcc agccgtcccc aaagaatgca
      361 gcgttaccgt aaatatcga
//
LOCUS       DAZFLD010000814          378 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.817, whole genome shotgun sequence.
ACCESSION   DAZFLD010000814 DAZFLD010000000
VERSION     DAZFLD010000814.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..378
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.817"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..117
                     /locus_tag="ACHKBL_005554"
     CDS             <1..117
                     /locus_tag="ACHKBL_005554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000817799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFO5822239.1"
                     /translation="VRTLCIRLYSLLQGKQASGRPWWYRHRTELPREWHGPE"
     gene            181..>378
                     /locus_tag="ACHKBL_005555"
     CDS             181..>378
                     /locus_tag="ACHKBL_005555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000842452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5710 domain-containing protein"
                     /protein_id="HFO5822240.1"
                     /translation="MKSRYRTWLAVPPEETEAVKNAVPPLNGRKAVAWDPEKKLWYAE
                     AGTDLSLLKRWLPRPQEMSMDA"
BASE COUNT           93 a          113 c           96 g           76 t
ORIGIN      
        1 gtccgcaccc tctgtatacg tctgtacagc ctgcttcagg gtaagcaggc atcgggacgt
       61 ccctggtggt accgacaccg cacagaatta ccccgtgaat ggcacggacc agaatagccc
      121 tcttttgtcc atcatcccgg cgtatcacaa cgccctcata tttaatcccc ggagaacgac
      181 atgaagtcca gatatcgtac ctggcttgct gtcccgcctg aggagacaga agccgtaaaa
      241 aatgcagtcc ccccgttaaa tggccgcaaa gcggtagcat gggatccaga gaaaaaactc
      301 tggtatgccg aggcgggtac cgacctttca ctgctgaagc gctggttacc ccgtccacag
      361 gaaatgtcga tggatgcc
//
LOCUS       DAZFLD010000815          378 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.818, whole genome shotgun sequence.
ACCESSION   DAZFLD010000815 DAZFLD010000000
VERSION     DAZFLD010000815.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..378
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.818"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..357)
                     /locus_tag="ACHKBL_005556"
     CDS             complement(<1..357)
                     /locus_tag="ACHKBL_005556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL_4695 family integrating conjugative element
                     protein"
                     /protein_id="HFO5822241.1"
                     /translation="MKRERQFYVLSLLLFCTTAGAELKVIADLGGRDASPFFEAINRQ
                     PPVPASVVPSPSFPQGEAAMLPVSTPEMSPGEVVARRLQLPGIGALFLVGDDAHSRTW
                     LQMNAAVLRERKAAGMV"
BASE COUNT           81 a          118 c           94 g           85 t
ORIGIN      
        1 taccattccc gccgccttcc gctccctgag tactgctgca ttcatctgca gccatgtgcg
       61 ggaatgggca tcatcgccca caagaaaaag tgcaccgatg cccggtaact gaagccggcg
      121 cgccacaacc tctccgggtg acatttcagg cgtactgacc ggtaacattg ccgcctctcc
      181 ctgcggaaaa gagggtgatg gcacaactga agcgggaacc ggtggctgcc ggtttatcgc
      241 ctcaaaaaag gggctggcat ccctgccgcc caggtctgca atcactttca gttctgcgcc
      301 agctgttgta cagaacagca gcagactcag tacataaaac tgtctttctc gtttcatttc
      361 tgctcctcct gtggttca
//
LOCUS       DAZFLD010000816          377 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.819, whole genome shotgun sequence.
ACCESSION   DAZFLD010000816 DAZFLD010000000
VERSION     DAZFLD010000816.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..377
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.819"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>377
                     /locus_tag="ACHKBL_005557"
     CDS             <1..>377
                     /locus_tag="ACHKBL_005557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001298206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFO5822242.1"
                     /translation="PSLENSHPTKTDNQLQIAVRNRMKIFYRPSNLSEDPNISYQKLR
                     WARKNEIVTVYNPGPRYVTLYNLHVDGKVMDGGMIAPFSHRQQSWCKAQGICEITWQT
                     LDIYNNVLPVWKVNMNPLQMDVS"
BASE COUNT          129 a           67 c           74 g          107 t
ORIGIN      
        1 taccatcgtt ggaaaatagt catccaacca aaactgataa tcaattgcaa atagcggtcc
       61 gcaatcgtat gaaaatattc tatcggccat caaatttgtc tgaagacccg aatatttcat
      121 atcaaaaatt acgctgggca cgaaaaaatg aaattgttac cgtttataat ccaggcccca
      181 gatatgtgac actctataac cttcatgttg atggtaaagt tatggatggt ggtatgatcg
      241 caccatttag ccatcgtcaa caatcatggt gtaaagcgca agggatttgt gaaataacat
      301 ggcagacatt ggacatttat aataatgtat tgcctgtatg gaaagtaaat atgaatccgt
      361 tgcaaatgga tgtgtca
//
LOCUS       DAZFLD010000817          376 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.820, whole genome shotgun sequence.
ACCESSION   DAZFLD010000817 DAZFLD010000000
VERSION     DAZFLD010000817.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..376
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.820"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>376
                     /locus_tag="ACHKBL_005558"
     CDS             <1..>376
                     /locus_tag="ACHKBL_005558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HFO5822243.1"
                     /translation="GWTPRGELAAVYDRSNTQVRSFTYDDKYRGRMVAHRHTGRPEIR
                     YRYDSDGRVTEQLNPAGLSYTYQYEKDHITITDSLDRREVLHTQGEGGLKRVVKKEHA
                     DGSVTQSQFDAVGRLKAQTDAAG"
BASE COUNT           93 a          103 c          129 g           51 t
ORIGIN      
        1 ggctggacgc cgcgcggcga actggcggcg gtgtatgacc gcagcaatac acaggtgcgc
       61 agctttactt acgatgataa ataccggggc cggatggtgg cgcaccgtca cacgggccgg
      121 ccggaaatcc gttaccgtta cgacagtgac gggcgggtga cagaacagct aaacccggca
      181 ggcttaagct acacgtatca gtatgagaaa gaccacatca ccatcaccga cagcctggac
      241 cgccgtgaag tgctgcacac gcagggcgaa ggcgggctga agcgggtggt gaaaaaggaa
      301 cacgcggacg gcagcgtcac gcagagtcag tttgacgcgg tgggcaggct taaggcacag
      361 acggatgccg caggcc
//
LOCUS       DAZFLD010000818          375 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.821, whole genome shotgun sequence.
ACCESSION   DAZFLD010000818 DAZFLD010000000
VERSION     DAZFLD010000818.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..375
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.821"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>375
                     /locus_tag="ACHKBL_005559"
     CDS             <1..>375
                     /locus_tag="ACHKBL_005559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5822244.1"
                     /translation="EYYWQTELGICKNNKFTPSGARLLIRVGSLRLNENHATKGTLQE
                     LLNTAEKTAFLGDRFRSYIRAGIYQKKSSDPAQLLAVLDNSIMVKYFKDEKPTYARMT
                     AHLPNKDESYECLTKVQHELLRS"
BASE COUNT          132 a           69 c           80 g           94 t
ORIGIN      
        1 gagtattatt ggcaaacaga acttggtata tgtaaaaata acaaattcac accaagcggg
       61 gcgagactct taattcgcgt cggatcacta cgccttaatg aaaatcatgc aaccaaagga
      121 acgttgcagg agttgctaaa tacagcagaa aaaacggcgt ttctcggcga tcggtttaga
      181 agttatataa gagcgggtat ctatcaaaaa aagagcagtg atccagctca gttgttggca
      241 gtccttgata attcaatcat ggttaaatat tttaaagatg agaagccaac ttatgctcga
      301 atgacagcac atctcccgaa caaagatgag tcttacgaat gtttaacaaa agtacagcat
      361 gaactgcttc gtagt
//
LOCUS       DAZFLD010000819          374 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.822, whole genome shotgun sequence.
ACCESSION   DAZFLD010000819 DAZFLD010000000
VERSION     DAZFLD010000819.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..374
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.822"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            24..100
                     /locus_tag="ACHKBL_005560"
     tRNA            24..100
                     /locus_tag="ACHKBL_005560"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:58..60,aa:Ile,seq:gat)
     gene            143..218
                     /locus_tag="ACHKBL_005561"
     tRNA            143..218
                     /locus_tag="ACHKBL_005561"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:176..178,aa:Ala,seq:tgc)
BASE COUNT          108 a           79 c           90 g           97 t
ORIGIN      
        1 aaaatgagca gtaaaacctc tacaggcttg tagctcaggt ggttagagcg cacccctgat
       61 aagggtgagg tcggtggttc aagtccactc aggcctacca aatttgcacg gcaaatttga
      121 agaggtttta actacatgtt atggggctat agctcagctg ggagagcgcc tgctttgcac
      181 gcaggaggtc tgcggttcga tcccgcatag ctccaccatc tctgtagtga ttaaataaaa
      241 aatacttcag agtgtacctg caaaggttca ctgcgaagtt ttgctcttta aaaatctgga
      301 tcaagctgaa aattgaaaca ctgaacaacg aaagttgttc gtgagtctct caaattttcg
      361 caacacgatg atga
//
LOCUS       DAZFLD010000820          372 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.823, whole genome shotgun sequence.
ACCESSION   DAZFLD010000820 DAZFLD010000000
VERSION     DAZFLD010000820.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..372
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.823"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>372
                     /locus_tag="ACHKBL_005562"
     CDS             <1..>372
                     /locus_tag="ACHKBL_005562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007370995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
                     /protein_id="HFO5822245.1"
                     /translation="HHPEFQKVESLWRGVKSLVDKTDFRRNVKIELLDLSKDDLRQDF
                     EDAPEIIQSGLYLQTYVAEYDTPGGEPIAALISAWEFDASAQDVALLKNISRVAASAH
                     MPFIGSVGPAFFRKDTMEEVAA"
BASE COUNT           77 a           80 c          116 g           99 t
ORIGIN      
        1 tcatcatccg gaatttcaga aagtggagtc cctgtggcgg ggcgtgaagt ctctggtgga
       61 taaaacggac ttccgccgta atgtgaaaat tgaactgctg gatctgtcca aagacgatct
      121 gcgtcaggat tttgaggatg cgccggaaat tattcagagt ggtctttatc tgcagactta
      181 tgtggcggag tacgatacgc cgggtggtga acccattgca gccctgattt ctgcctggga
      241 gtttgatgcg tctgcgcagg atgtggcgct gctgaaaaat atttcccggg tggctgcgtc
      301 cgcgcatatg ccatttattg gttcagttgg cccggctttc ttccggaagg atacgatgga
      361 agaggtggcc gc
//
LOCUS       DAZFLD010000821          371 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.824, whole genome shotgun sequence.
ACCESSION   DAZFLD010000821 DAZFLD010000000
VERSION     DAZFLD010000821.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..371
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.824"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>371)
                     /locus_tag="ACHKBL_005563"
     CDS             complement(<1..>371)
                     /locus_tag="ACHKBL_005563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001279669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication endonuclease"
                     /protein_id="HFO5822246.1"
                     /translation="GAVMRLSCATWWNSQLRHLRDIWREELLRAAGLVSRKTSVYISH
                     EALLDWREKRQRTRDFLKAWDIENEEGERFSLEDIYWAGLSNPRNRRNEMMTCARGME
                     QVAEARGDVAFFVTVTAPSRF"
BASE COUNT           76 a          112 c          101 g           82 t
ORIGIN      
        1 tgaaaacggg aaggggctgt gacggtgacg aaaaacgcca cgtcgccgcg tgcttcggcg
       61 acctgctcca ttccccgggc gcaggtcatc atctcgttac ggcggttacg cggattagag
      121 agacctgccc agtagatatc ctcaaggctg aaccgttctc cctcttcgtt ctcgatgtcc
      181 caggccttca gaaaatcgcg ggtgcgctgg cgtttttcgc gccagtccag taacgcttca
      241 tggctgatat ataccgatgt cttacgtgac accagacctg cggcgcgtaa cagttcttca
      301 cgccagatgt cccggaggtg tcttaactga ctgttccacc atgtcgcaca ggaaaggcgc
      361 atgaccgcac c
//
LOCUS       DAZFLD010000822          370 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.825, whole genome shotgun sequence.
ACCESSION   DAZFLD010000822 DAZFLD010000000
VERSION     DAZFLD010000822.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..370
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.825"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
BASE COUNT          112 a           63 c           76 g          119 t
ORIGIN      
        1 tattggttga ttatgtaata tcaccggctg atgatattaa aaacgtcatg atgttgtatg
       61 gaaataatgc tgaaaacaag aacagacgca gactgatcta caacgcatta accatcattt
      121 gtggtgatga tactaaaaac aaccagtcag accctagatg atattatcgg aacgtgtttt
      181 tggcatccga tgtttgtata tccctgctat tgttgctagt cggctagcaa caatagcaga
      241 gggggcatgc atgtctgaaa aaataaaaaa tcgcaacaca ttgattttta tcttgtttgc
      301 attttgcaaa tactatctct cgtttttgtg attagtgtca tgttttatcg tctcgcggat
      361 aaccgtgagg
//
LOCUS       DAZFLD010000823          369 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.826, whole genome shotgun sequence.
ACCESSION   DAZFLD010000823 DAZFLD010000000
VERSION     DAZFLD010000823.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..369
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.826"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(144..285)
                     /locus_tag="ACHKBL_005564"
                     /pseudo
     CDS             complement(144..285)
                     /locus_tag="ACHKBL_005564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014543172.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS481 family transposase"
BASE COUNT          100 a           84 c           90 g           95 t
ORIGIN      
        1 gagattattc ttacggggcc gggcgaatgc tatctgaatc gtacggttca tagaaataaa
       61 tatttaccga catagccgga gggttttcag atgcaatccc tgagtcactg cgaaatcgca
      121 tcgtatccga tcgcggactc gccgattctc agaacaggca gtccctgaga atcgcagaac
      181 gctagtgcac gatcgttgaa cacatcagct acggtgatcg atgttttact cgtattatag
      241 tttgcagtgt gccactttcg gggatgtagc tacgaacgtc tgctggcttc ccgatatgtc
      301 agtaaaaaat acccgccgta ttacagcaac ggcaggtaat ggcacatcca gaagagaagt
      361 attttatta
//
LOCUS       DAZFLD010000824          368 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.827, whole genome shotgun sequence.
ACCESSION   DAZFLD010000824 DAZFLD010000000
VERSION     DAZFLD010000824.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..368
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.827"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..231
                     /locus_tag="ACHKBL_005565"
     CDS             <1..231
                     /locus_tag="ACHKBL_005565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5822247.1"
                     /translation="KSRAVHTVRQAPFVLTGTELRGGSTRAAVAPRGYTSLSQVALLG
                     EGESVAGWTLVYAGHGEATFRVNGRQTVLRVE"
BASE COUNT           71 a           95 c          127 g           75 t
ORIGIN      
        1 aaatcccgcg cagttcatac agttcggcag gctccttttg tgctgaccgg gacagagctg
       61 cgcggtggca gtacccgtgc agccgtggct ccccgggggt ataccagcct ctctcaggtg
      121 gctctgctgg gtgaaggaga gagtgtggcc gggtggacgc ttgtgtatgc cggccatggt
      181 gaagcaacgt tccgggtgaa tggtcgtcag acggtgctca gggtggagta gatgatgaag
      241 cgaaaaatac tgtatctgct ggcactgtgc cccgttctgg ctcagtccgc cgggcagacg
      301 aatatgtcgt cagaggcgca acggacgacc ggtacggtgg tgacgcagag taccacatcc
      361 ggacttca
//
LOCUS       DAZFLD010000825          367 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.828, whole genome shotgun sequence.
ACCESSION   DAZFLD010000825 DAZFLD010000000
VERSION     DAZFLD010000825.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..367
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.828"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(169..>367)
                     /locus_tag="ACHKBL_005566"
     CDS             complement(169..>367)
                     /locus_tag="ACHKBL_005566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000425544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5822248.1"
                     /translation="LNGDATPNIRTMSNKKIECLEKSEVEWASRVCDSITDKERENLK
                     ALICSKPAKSVNTGKRPALGY"
BASE COUNT           74 a           87 c           74 g          132 t
ORIGIN      
        1 acaaaaaagc agcgttgcgt gatctttccc tagtccctgt cccggattgt agtaccggtg
       61 ccagttgtag tgcagttcgg attcgtcgtc atactgctag cccggatggc ggagcagttg
      121 ctctagtcac tcagaatatt gtaatagtga tagagtgcag tgccggtttc aatatcctaa
      181 cgctggtctt ttgcctgtat taactgattt agctggtttg ctacatatta atgccttcag
      241 attttccctt tccttgtctg tgatactgtc gcaaacccta ctagcccatt ctacttcact
      301 cttctccagg cattctattt tcttattgct cattgttcta atgtttggtg ttgcatctcc
      361 atttaat
//
LOCUS       DAZFLD010000826          367 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.829, whole genome shotgun sequence.
ACCESSION   DAZFLD010000826 DAZFLD010000000
VERSION     DAZFLD010000826.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..367
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.829"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..270
                     /locus_tag="ACHKBL_005567"
     CDS             <1..270
                     /locus_tag="ACHKBL_005567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001599750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5822249.1"
                     /translation="TLKNRKEFLSSVINVNETRLQASAPAMPAIEALNAFIDKGDYLK
                     SLYVNKTRTAAEQKRLNEILRAQVISGLKASEGLAKLLIASGIML"
BASE COUNT          124 a           59 c           84 g          100 t
ORIGIN      
        1 actctgaaaa acaggaagga gtttctaagt tcagtaatca atgtgaatga aaccagacta
       61 caggcatcgg cacctgccat gcctgcgatt gaggctttga atgcctttat agataagggt
      121 gattatctga aaagcttata cgttaataaa acacgcacgg cagctgaaca aaaaagatta
      181 aatgaaatat tacgcgcaca ggtcattagt gggttaaagg cgtctgaggg gctggcaaaa
      241 ttacttattg ccagcggaat aatgttatag gaggcttttt atgaacgatg tgcaaaaccg
      301 tagaatactg gcattacttg atatgctgta taatactttg ggtaataaaa ccacatgtaa
      361 tgtttgt
//
LOCUS       DAZFLD010000827          366 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.830, whole genome shotgun sequence.
ACCESSION   DAZFLD010000827 DAZFLD010000000
VERSION     DAZFLD010000827.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..366
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.830"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..320
                     /locus_tag="ACHKBL_005568"
     CDS             <1..320
                     /locus_tag="ACHKBL_005568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000977717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TIGR03759 family integrating conjugative element
                     protein"
                     /protein_id="HFO5822250.1"
                     /translation="KTGALTGDTGGRLALFVKAKECGTCDARLASVLATGRQVDIYLV
                     DSQGKDEILRQWAHAHSIPVEKVRSRHITLNHDAGRWLRFGEGKMPVVLQQGADGWRV
                     AAF"
BASE COUNT           72 a           84 c          130 g           80 t
ORIGIN      
        1 gaaaaacagg agcacttacc ggagatacgg ggggacggct ggcgctgttt gtgaaggcga
       61 aggaatgtgg cacctgtgat gcccgtcttg cctccgtact ggccacggga cggcaggttg
      121 atatctacct tgtcgacagt cagggaaagg atgagattct gcgtcagtgg gcgcatgcgc
      181 acagcatacc tgtggagaaa gttcgcagcc gtcatatcac cctgaatcat gatgccggac
      241 gctggctgcg ttttggtgaa ggaaaaatgc cggtggtact gcagcagggg gctgacggat
      301 ggcgcgtggc ggcattctga cggttctgct gatggtggcc tgcagtggtg tctgtcgtgc
      361 tgccgt
//
LOCUS       DAZFLD010000828          360 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.831, whole genome shotgun sequence.
ACCESSION   DAZFLD010000828 DAZFLD010000000
VERSION     DAZFLD010000828.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..360
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.831"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(59..>360)
                     /locus_tag="ACHKBL_005569"
     CDS             complement(59..>360)
                     /locus_tag="ACHKBL_005569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004160261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HFO5822251.1"
                     /translation="EAEGWLPGGVLHTDLLVLLRVYLGYRSDVRLRLTVPVRLLPEPR
                     LGKGRPVQLGRTGLLGLKAGKLSNGRESLTVSLGCYEGLKCAALPSAEDGHYRFE"
BASE COUNT           84 a          115 c           96 g           65 t
ORIGIN      
        1 cccaccagcg gattactgtt accattcagg cgttccttta ttgagagaga acaggtgatt
       61 attcgaagcg ataatgcccg tcttcagcag acggtagcgc agcacatttc agcccttcgt
      121 agcaacccag actgaccgtc agactctccc ggccattgct gagtttgccc gctttcagcc
      181 ccagtaaacc ggtacggccc agctgaacag gcctgccttt gcccaggcgg ggctcaggca
      241 acaaccgcac ggggacagta aggcgcagcc ggacgtcact gcggtagccg agataaacgc
      301 gcagcaggac cagcaaatcg gtatggagaa cacctccggg caaccagcct tccgcctcgt
//
LOCUS       DAZFLD010000829          358 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.832, whole genome shotgun sequence.
ACCESSION   DAZFLD010000829 DAZFLD010000000
VERSION     DAZFLD010000829.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..358
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.832"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>358)
                     /locus_tag="ACHKBL_005570"
     CDS             complement(<1..>358)
                     /locus_tag="ACHKBL_005570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShET2/EspL2 family type III secretion system
                     effector toxin"
                     /protein_id="HFO5822252.1"
                     /translation="KTIQPTVNLNGSAFFSQSPSDAIFCRHLSLQYALDSLRNGKGKV
                     NLIKHYSSVESIQQHVPLVRDAEFRSLLRHPPAGSRVIASKDFGFALDIFFCRMMANN
                     VSHMSAILYIDNHTLSV"
BASE COUNT          114 a           66 c           81 g           97 t
ORIGIN      
        1 ttactgacaa agtatgattg tctatatata aaatcgcgga catatgactg acattgtttg
       61 ccatcattcg acagaagaaa atatctaaag cgaagccaaa atccttactc gcgataacgc
      121 gactccctgc aggaggatgg cgaagtaatg atctgaactc cgcgtcccgg actaagggaa
      181 catgctgctg tatggattca acggaggagt aatgtttaat cagattgact ttgccttttc
      241 catttctcag tgaatcaagg gcgtattgca atgacagatg gcgacaaaaa atagcgtcag
      301 aaggagattg cgaaaaaaat gcactaccat tgagatttac tgttggttgt atagtttt
//
LOCUS       DAZFLD010000830          357 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.833, whole genome shotgun sequence.
ACCESSION   DAZFLD010000830 DAZFLD010000000
VERSION     DAZFLD010000830.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..357
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.833"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>357)
                     /locus_tag="ACHKBL_005571"
     CDS             complement(<1..>357)
                     /locus_tag="ACHKBL_005571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5822253.1"
                     /translation="IDITGIGKGVFDIVSRFAPREANAILYSVESKNRLVMKMIDVVA
                     HKRIEWAKDAIDEASRERTEIPASFMSIRRTTTKSGNALTFVAERSDVTGHADVFFAI
                     SHAVINEPVDYDYERPS"
BASE COUNT           79 a          106 c           87 g           85 t
ORIGIN      
        1 ggacgggcgt tcatagtcat aatctacagg ctcattaatc acggcgtgcg aaatggcaaa
       61 aaacacatcc gcatgccccg taacatccga ccgttccgcc acgaacgtca gcgcgttacc
      121 gctttttgtg gtggtgcggc gtatggacat gaacgacgcg gggatctccg tgcgttcacg
      181 gctggcctca tcaatggcgt ctttcgccca ctcgatacgc ttatgggcca ccacgtctat
      241 cattttcatc accaggcggt ttttgctctc cacgctgtaa agaatggcgt tagcctcacg
      301 tggcgcaaag cgactgacga tatcaaacac ccctttcccg ataccggtga tatctat
//
LOCUS       DAZFLD010000831          353 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.834, whole genome shotgun sequence.
ACCESSION   DAZFLD010000831 DAZFLD010000000
VERSION     DAZFLD010000831.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..353
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.834"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
BASE COUNT           99 a           70 c           97 g           87 t
ORIGIN      
        1 atctggaaag tagatcgcag aaaataaaag aacctgctgg tgaggcaggt tcttttattt
       61 actactttga tgttaagtta ctgatagtgc aacatggcat ctcaacgaac gaattataac
      121 caccgctgga ccgtgttgca attaagataa tgagatttat aatcctgact gttgggaagt
      181 tgcgccggat cgaacccatt tcaagtcctg gctaccggac gggtggtgcc gcaggtagtg
      241 ctctgatccg gagtgcaaca tggcgactca acaaacgtaa accagtgtcg acaggcagtg
      301 taagggtaag gtgctggtgt tgcgctcctt aataaggaga gcggatggac acg
//
LOCUS       DAZFLD010000832          353 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.835, whole genome shotgun sequence.
ACCESSION   DAZFLD010000832 DAZFLD010000000
VERSION     DAZFLD010000832.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..353
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.835"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..351
                     /locus_tag="ACHKBL_005572"
     CDS             <1..351
                     /locus_tag="ACHKBL_005572"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5822254.1"
                     /translation="LSLIKKGNNMRNITTDELALIHGAGLDKDASVAIGTAAGGFLGK
                     ATKIPSADIAGSAIGGYIGGVIADGSNRTITIPSNGINYNPSIGLGSFNPNYNGGLLN
                     SGFNSSTSSSSSGS"
BASE COUNT          126 a           51 c           74 g          102 t
ORIGIN      
        1 ctttctttaa taaaaaaagg aaataacatg agaaatataa caaccgatga acttgcttta
       61 atacatggtg caggattaga taaagatgca tccgttgcaa taggtactgc ggctggtggt
      121 tttttaggta aagcaacaaa aatccctagt gcggatatag caggaagtgc aattggagga
      181 tatattggag gagttattgc tgatggttca aataggacaa taacaatacc atcaaacggt
      241 attaactata atccgagtat aggacttgga agttttaatc caaactacaa tggaggttta
      301 ttaaactcag gatttaactc atcaacatcc tcttctagta gtggaagttg att
//
LOCUS       DAZFLD010000833          352 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.836, whole genome shotgun sequence.
ACCESSION   DAZFLD010000833 DAZFLD010000000
VERSION     DAZFLD010000833.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..352
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.836"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>352
                     /locus_tag="ACHKBL_005573"
     CDS             <1..>352
                     /locus_tag="ACHKBL_005573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFO5822255.1"
                     /translation="PVLPDVREFIRGTVNVMENLMPYRELGIPLPKVRTLVNCMDYTA
                     LARQTLAELTGIINAGRYSKVLPEGAVTLLSTQIYDLNIYKQGHAAGQPVHRLEKVSA
                     RCSDSALVTMHSLAC"
BASE COUNT           77 a          100 c           96 g           79 t
ORIGIN      
        1 ccggttctgc cggatgtgcg tgagttcatc cgcggcacgg tcaatgtcat ggaaaacctt
       61 atgccctacc gtgaactggg tattcccctg ccgaaagtcc gcaccctggt taactgcatg
      121 gattatacgg cgctggccag acagacactg gcagagctga caggcattat caacgccggt
      181 cgctacagca aagtgctgcc tgaaggtgcg gtcacccttc tttcaacaca gatttacgac
      241 ctgaatattt ataaacaggg gcacgcagcc ggtcagccgg tgcatcgtct ggaaaaagtg
      301 tctgcccgtt gcagcgattc tgcgctggtc accatgcaca gtctggcctg tg
//
LOCUS       DAZFLD010000834          351 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.837, whole genome shotgun sequence.
ACCESSION   DAZFLD010000834 DAZFLD010000000
VERSION     DAZFLD010000834.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..351
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.837"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>351
                     /locus_tag="ACHKBL_005574"
     CDS             <1..>351
                     /locus_tag="ACHKBL_005574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5822256.1"
                     /translation="RIPELSALPPSLRKKNFTDAYNAASSHIEYWIGAGVSFISMMIL
                     SRVYDFLLPTQDTFPGDIIRSLCVVCPSILIWFQFSVYVMRKYYRHILLRGKETETIS
                     ERLIREADTREYELW"
BASE COUNT          104 a           68 c           67 g          112 t
ORIGIN      
        1 cgcattcctg aactttcagc acttccacct tcattaagaa agaaaaattt tactgacgca
       61 tataatgcag catcttctca tatcgaatac tggataggtg ctggcgtctc atttatctcg
      121 atgatgattt tatccagagt atatgacttt cttttaccca cccaagatac tttcccgggt
      181 gatatcatca gaagtttatg tgttgtgtgt ccatccatat taatctggtt tcagttttcg
      241 gtatatgtca tgaggaaata ttacagacat attctgctgc gaggaaaaga aaccgaaacc
      301 atttcagaaa ggttgatacg tgaggctgat acacgagaat atgaactatg g
//
LOCUS       DAZFLD010000835          338 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.838, whole genome shotgun sequence.
ACCESSION   DAZFLD010000835 DAZFLD010000000
VERSION     DAZFLD010000835.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..338
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.838"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>338
                     /locus_tag="ACHKBL_005575"
     CDS             <1..>338
                     /locus_tag="ACHKBL_005575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000842452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HFO5822257.1"
                     /translation="LDGRPSGWYRDYRSADNSPTTWTFSRAENIDPLARLHLKASAQQ
                     NRDEKERKLQQQYNKQAGYARSYLNGLPQATAHEYLTRKGIRAAPGVRLNNKNELVIP
                     FSNGRGEIRS"
BASE COUNT           91 a          104 c           84 g           59 t
ORIGIN      
        1 cttgatggcc gcccctccgg ctggtaccgc gattaccgga gcgctgataa cagtcccaca
       61 acctggacat tctcccgcgc agaaaatatc gatcctctcg ccagactgca tctgaaggca
      121 tccgctcagc aaaatcgcga tgagaaagaa cgtaaactac agcaacagta caacaaacag
      181 gccggctatg cccgctccta tcttaacggg ttgccccagg caacagcaca tgaatacctg
      241 acacgcaagg gcatccgggc cgcgcctggc gtgcggctga acaataaaaa tgagctggtt
      301 attcccttca gcaacggccg gggggaaata cgttcgta
//
LOCUS       DAZFLD010000836          336 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.839, whole genome shotgun sequence.
ACCESSION   DAZFLD010000836 DAZFLD010000000
VERSION     DAZFLD010000836.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..336
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.839"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>336
                     /locus_tag="ACHKBL_005576"
     CDS             <1..>336
                     /locus_tag="ACHKBL_005576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5822258.1"
                     /translation="KQAGNTQYDYDAAGRMVSRTRHRDGYRPETERFRWDSRDQLTGY
                     CSAQGEQWEYRYDASGRRTEKRCDRKKIRFTYLWDGDNIAEIREYRDDKLYSVRHLVF
                     NGFELISQQ"
BASE COUNT           88 a           89 c          109 g           50 t
ORIGIN      
        1 gaaacaggcc ggaaacacgc agtatgacta tgacgccgca ggccggatgg tcagccgcac
       61 cagacaccgt gacggctacc gcccggaaac agagcggttc cggtgggaca gccgtgacca
      121 gctgaccggg tattgcagcg cacagggtga gcagtgggaa taccgctacg acgccagcgg
      181 caggcggacg gaaaaacgct gcgaccggaa gaaaatccgt tttacgtacc tgtgggacgg
      241 cgacaatatt gcggaaatcc gggaataccg cgatgataaa ctgtacagcg tacggcacct
      301 ggtgtttaac ggctttgagc tgataagcca gcagtg
//
LOCUS       DAZFLD010000837          335 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.840, whole genome shotgun sequence.
ACCESSION   DAZFLD010000837 DAZFLD010000000
VERSION     DAZFLD010000837.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..335
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.840"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>335
                     /locus_tag="ACHKBL_005577"
     CDS             <1..>335
                     /locus_tag="ACHKBL_005577"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFO5822259.1"
                     /translation="NRVPYASYLNKIVADYNLSDYVEFMGFRHDLDQIRLDNDVCLIP
                     SLSEAFGRVTIESMAAGMIVVASDSGASKEIINDGINGFLFSSGSVSDLTSVLKKILD
                     VESNNLECI"
BASE COUNT           98 a           39 c           72 g          126 t
ORIGIN      
        1 aatagagttc catatgcaag ttatcttaat aaaattgttg ctgattataa tttgagtgat
       61 tatgtagagt ttatgggatt taggcatgat cttgatcaaa taaggcttga caacgatgtt
      121 tgcttaattc cctctctttc tgaagctttt ggtagagtta ccattgaatc aatggctgca
      181 ggtatgatag ttgttgctag tgattctggg gctagtaaag agatcatcaa tgatggtata
      241 aatggttttt tgttttcttc aggttcggta agtgatctta ctagcgtact taaaaaaatc
      301 cttgatgtag agtctaataa tttagaatgt ataag
//
LOCUS       DAZFLD010000838          332 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.842, whole genome shotgun sequence.
ACCESSION   DAZFLD010000838 DAZFLD010000000
VERSION     DAZFLD010000838.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..332
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.842"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>332)
                     /locus_tag="ACHKBL_005578"
     CDS             complement(<1..>332)
                     /locus_tag="ACHKBL_005578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000118787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2586 family protein"
                     /protein_id="HFO5822260.1"
                     /translation="ITAKDAVNRATETRALLQSAYGRFVWFMLCVAGPGKDEAWAAYV
                     TRISAIQDGVAAPGVMVVPRLWGNEPGVLAGRLCNPSVTVADSPARVATGALVAMGND
                     EIPQDGKK"
BASE COUNT           63 a          109 c           99 g           61 t
ORIGIN      
        1 tgtttcttgc cgtcctgcgg gatctcatcg ttacccatcg ccaccagggc accggttgcc
       61 acacgtgccg gactgtccgc aacggtgacg gaaggattac agagacggcc cgccagtacg
      121 ccgggctcat ttccccataa gcggggaacc accatcacgc caggtgctgc cacgccgtcc
      181 tgtatggcac tgatgcgggt gacgtatgcc gcccatgcct catccttgcc cggtccggcc
      241 acgcacagca tgaaccagac aaaacggcca taagcggact gaagcagggc gcgggtttcg
      301 gtggcgcggt taacggcatc tttggccgtg at
//
LOCUS       DAZFLD010000839          331 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.843, whole genome shotgun sequence.
ACCESSION   DAZFLD010000839 DAZFLD010000000
VERSION     DAZFLD010000839.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..331
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.843"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
BASE COUNT           99 a           62 c           91 g           79 t
ORIGIN      
        1 atactcattt tggttactta aatgggtgaa tgcagaaagg ttgctctggt ggtcaacgtg
       61 atgacaaaga caggtaagac agggactcac taaacctaaa tggggttttg ggcttgaatc
      121 ccgcgcagta aatgaggtgt gagtcagcga attacatagg ggctcgcagc tttatggttg
      181 caataaagcc ggatccccgg gggaatagat ttaagcccag caggcacgtc gaccaacgaa
      241 tatcgtcatc atagagctgg caaataagat tgccaggatc gcatgggcag tgtttatgcg
      301 tggtgaaaaa cttatatcag caaccagtta a
//
LOCUS       DAZFLD010000840          330 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.844, whole genome shotgun sequence.
ACCESSION   DAZFLD010000840 DAZFLD010000000
VERSION     DAZFLD010000840.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..330
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.844"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     repeat_region   34..306
                     /inference="COORDINATES: alignment:crt:1.2"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=34..62
                     /rpt_unit_seq="gagttccccgcgccagcggggataaaccg"
BASE COUNT           68 a           94 c          100 g           68 t
ORIGIN      
        1 gagatttcag aggcagccgc caggccgttg atcgagttcc ccgcgccagc ggggataaac
       61 cgccatttcg gtagcgccat caaaatggtt acgcgagttc cccgcgccag cggggataaa
      121 ccgcgtcgtt agcggtctgg tgacgttaaa ggggtgagtt ccccgcgcca gcggggataa
      181 accgttgatc gccgtggcgg tgagattaga aacgcggagt tccccgcgcc agcggggata
      241 aaccggaagt ggcaaagctg ccgtttcttt tctctatgag ttccccgcgc ctgcggggat
      301 aaaccaccat ataacccgtt atctctttct
//
LOCUS       DAZFLD010000841          329 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.845, whole genome shotgun sequence.
ACCESSION   DAZFLD010000841 DAZFLD010000000
VERSION     DAZFLD010000841.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..329
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.845"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>329
                     /locus_tag="ACHKBL_005579"
     CDS             <1..>329
                     /locus_tag="ACHKBL_005579"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5822261.1"
                     /translation="IKVLAKSIAENGTSPLETLAVMENENGHFIALEGNRRVCALTLL
                     NDPDLCPENERAYFRKLSDNSDVIPDAVECVLFENREDANKWIELRHNGEQDGAGLLT
                     WNATQKA"
BASE COUNT          111 a           46 c           81 g           91 t
ORIGIN      
        1 attaaagtat tggctaaaag tattgctgag aatggaacaa gtccattaga aacacttgcg
       61 gtgatggaga acgaaaatgg acactttatt gcccttgagg ggaataggag agtttgtgca
      121 ttaactctgc ttaatgatcc tgatttatgt ccagaaaatg agagggctta tttcagaaaa
      181 ctatctgata atagtgacgt cattcctgat gcagttgaat gcgttttatt tgaaaataga
      241 gaggatgcaa ataaatggat tgaattgcgc cataatggag aacaagacgg tgcggggctt
      301 cttacttgga atgcaactca aaaagcaag
//
LOCUS       DAZFLD010000842          329 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.846, whole genome shotgun sequence.
ACCESSION   DAZFLD010000842 DAZFLD010000000
VERSION     DAZFLD010000842.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..329
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.846"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>329
                     /locus_tag="ACHKBL_005580"
     CDS             <1..>329
                     /locus_tag="ACHKBL_005580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000623204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5822262.1"
                     /translation="LPRLLAVREMPGQDAVKVYFRNGNHTSGRSRISAGAVGWVHHNG
                     AEIRVSADSFSGQDQNGQTATRRQAKRLRELGFKVRQKGRWRKASMKYLLTLSRAQAG
                     LLITIME"
BASE COUNT           73 a           92 c          108 g           56 t
ORIGIN      
        1 ctgccgcgtc ttctggcggt ccgtgagatg cccggacagg atgccgtaaa ggtttatttc
       61 cgtaacggca atcacaccag tggcaggagc cgcatcagtg ccggtgccgt gggctgggtt
      121 caccacaacg gcgcagaaat ccgggtcagt gctgacagct tcagcggtca ggaccagaac
      181 gggcagaccg ccacccgccg acaggcaaaa cgactgcggg agctgggctt taaggtacgc
      241 cagaaggggc gctggcggaa agcatccatg aaatacctgt taacgctttc gcgggcgcag
      301 gccgggttgc tgatcaccat tatggaaga
//
LOCUS       DAZFLD010000843          327 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.847, whole genome shotgun sequence.
ACCESSION   DAZFLD010000843 DAZFLD010000000
VERSION     DAZFLD010000843.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..327
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.847"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(121..>327)
                     /locus_tag="ACHKBL_005581"
     CDS             complement(121..>327)
                     /locus_tag="ACHKBL_005581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000914970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5822263.1"
                     /translation="VKMQAAGKVAEVSEAAQSGLSEVKDAVEEVKTQAAGKVAEVSEA
                     TQSGLSDVKDTAPEKGAEISVTEE"
BASE COUNT           69 a          101 c           70 g           87 t
ORIGIN      
        1 cgcaggtaca gggcatcgga tatccggtaa atatgctgat ttattaaaga gacagacatg
       61 ccccgtatat aaaattccgt gtcaccgggt gctgaaaaaa actgcaccgg cggcgctctt
      121 ttactcctcc gttacggata tttcagcccc cttttcagga gcggtatcct tcacatcact
      181 gagaccggac tgagtcgctt cagagacctc tgccaccttt ccggctgcct gtgtttttac
      241 ttcctccacg gcatccttca cctcgctgag cccggactga gctgcttcag aaacctctgc
      301 cacttttccg gcagcctgca ttttcac
//
LOCUS       DAZFLD010000844          326 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.848, whole genome shotgun sequence.
ACCESSION   DAZFLD010000844 DAZFLD010000000
VERSION     DAZFLD010000844.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..326
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.848"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            46..326
                     /locus_tag="ACHKBL_005582"
                     /pseudo
     CDS             46..326
                     /locus_tag="ACHKBL_005582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238907.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT           81 a           67 c           91 g           87 t
ORIGIN      
        1 ctggcgtcag tccttgctgg gagcgggctt tcgtaatctt tccctgggcg gtatatcatc
       61 caggactcga ttgggctgaa ggggggatgg aatctgtacc aatatccgct gaatccagtg
      121 caacacgttg atccattagg gcttagtact atgataatag gtaacggccc cgttcctgat
      181 aatccttttg ggcatgctgc ggcggcaaat cgatatggat taatgagttc gggaactggg
      241 gatgagatgg gagcctctgt ctctgattat tttaaaaaga tgcaaccccg cagagatacc
      301 tggatttgga taatagatac aacaga
//
LOCUS       DAZFLD010000845          322 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.849, whole genome shotgun sequence.
ACCESSION   DAZFLD010000845 DAZFLD010000000
VERSION     DAZFLD010000845.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..322
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.849"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>322
                     /locus_tag="ACHKBL_005583"
     CDS             <1..>322
                     /locus_tag="ACHKBL_005583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004173387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImcF-related family protein"
                     /protein_id="HFO5822264.1"
                     /translation="VRTRRDEIDWVLTDKTHQTGSDISPEALKIRLTERYFTDFGNAW
                     LNMLNSIQWHEPTSLSGAIAQLSLLGDVRQSPLVALMNTLNYQGKTGQKGEALADSVV
                     DSAKK"
BASE COUNT           82 a           80 c           92 g           68 t
ORIGIN      
        1 gtcagaacac gccgcgatga aattgactgg gtactgacgg ataagacgca tcagacaggg
       61 agcgatatct ctccggaggc gctgaagatc cgcctgactg agcgctattt taccgatttt
      121 ggcaatgcct ggctgaacat gctcaacagc atacagtggc atgaacccac gtcactttcc
      181 ggagccattg cccagttgag tctgctcggt gatgtccgtc agtctccgtt agttgcactg
      241 atgaacacgc tgaactacca ggggaaaacg gggcagaaag gtgaggcact ggctgattca
      301 gtcgtggatt cagcgaaaaa gc
//
LOCUS       DAZFLD010000846          321 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.850, whole genome shotgun sequence.
ACCESSION   DAZFLD010000846 DAZFLD010000000
VERSION     DAZFLD010000846.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..321
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.850"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>321
                     /locus_tag="ACHKBL_005584"
     CDS             <1..>321
                     /locus_tag="ACHKBL_005584"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFO5822265.1"
                     /translation="INAIILHRTDSSTINGTLASFSKGIGTHFIVEKDGNIYQAASLF
                     VYTSHVGKIKSRCKEEKTWSSEEKKKTEAFGWNAKKIHDHEKIKQYPYRYPYNMDSVG
                     IEVIA"
BASE COUNT          124 a           42 c           65 g           90 t
ORIGIN      
        1 aattaatgca ataatattgc atagaacaga ttcttcaaca ataaatggta cacttgctag
       61 tttttctaaa ggcattggaa ctcattttat tgttgaaaaa gatggtaata tataccaggc
      121 tgcaagccta tttgtatata ccagccatgt ggggaagata aagtctcgat gtaaggagga
      181 aaaaacatgg tcgtctgaag aaaaaaagaa aacagaggca ttcggatgga atgctaagaa
      241 gatacatgat catgaaaaaa ttaaacaata tccttataga tatccatata atatggacag
      301 tgttggcatt gaggttattg c
//
LOCUS       DAZFLD010000847          320 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.851, whole genome shotgun sequence.
ACCESSION   DAZFLD010000847 DAZFLD010000000
VERSION     DAZFLD010000847.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..320
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.851"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>320
                     /locus_tag="ACHKBL_005585"
     CDS             <1..>320
                     /locus_tag="ACHKBL_005585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004173387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImcF-related family protein"
                     /protein_id="HFO5822266.1"
                     /translation="VRTRRDEIDWVLTDKTHQTGSDISPEALKSRLTERYFTDFGNAW
                     LNMLNSIQWHEPTSLSGAIAQLSLLGDVRQSPLVALMNTLNYQGKTGQKGEALADSVV
                     DSAK"
BASE COUNT           84 a           81 c           90 g           65 t
ORIGIN      
        1 gtcagaacac gccgcgatga aattgactgg gtactgacgg ataaaacaca tcagacaggg
       61 agcgatatct ctccggaggc cctgaagagc cgcctgactg aacgctattt taccgatttt
      121 ggtaatgcct ggctgaacat gctcaacagc atacagtggc atgaacccac gtcactttcc
      181 ggagccattg cccagttgag tctgctcggt gacgtccgcc agtctccgtt agttgcactg
      241 atgaacacgc tgaactacca ggggaaaacg gggcagaagg gggaggcact ggctgattca
      301 gtcgtggatt cagcgaaaaa
//
LOCUS       DAZFLD010000848          317 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.852, whole genome shotgun sequence.
ACCESSION   DAZFLD010000848 DAZFLD010000000
VERSION     DAZFLD010000848.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..317
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.852"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>317
                     /locus_tag="ACHKBL_005586"
     CDS             <1..>317
                     /locus_tag="ACHKBL_005586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HFO5822267.1"
                     /translation="AIDKPFLLPIEDVFSISGRGTVVTGRVERGIIKVGEEVEIVGIK
                     ETQKSTCTGVEMFRKLLDEGRAGENVGVLLRGIKREEIERGQVLAKPGTIKPHTKFES
                     EVY"
BASE COUNT           80 a           71 c           90 g           76 t
ORIGIN      
        1 gcgattgaca agccgttcct gctgccgatc gaagacgtat tctccatctc cggtcgtggt
       61 accgttgtta ccggtcgtgt agaacgcggt atcatcaaag ttggtgaaga agttgaaatc
      121 gttggtatca aagagactca gaagtctacc tgtactggcg ttgaaatgtt ccgcaaactg
      181 ctggacgaag gccgtgctgg tgagaacgta ggtgttctgc tgcgtggtat caaacgtgaa
      241 gaaatcgaac gtggtcaggt actggctaag ccgggcacca tcaagccgca caccaagttc
      301 gaatctgaag tgtacat
//
LOCUS       DAZFLD010000849          315 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.853, whole genome shotgun sequence.
ACCESSION   DAZFLD010000849 DAZFLD010000000
VERSION     DAZFLD010000849.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..315
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.853"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            10..141
                     /locus_tag="ACHKBL_005587"
     CDS             10..141
                     /locus_tag="ACHKBL_005587"
                     /inference="COORDINATES: protein motif:HMM:NF022230.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage protein"
                     /protein_id="HFO5822268.1"
                     /translation="MNISAGEAGKTTKKIKFMVTSPDFISIDGVPYLSARDTRDLKG"
     gene            146..>315
                     /locus_tag="ACHKBL_005588"
     CDS             146..>315
                     /locus_tag="ACHKBL_005588"
                     /inference="COORDINATES: protein motif:HMM:NF016437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HFO5822269.1"
                     /translation="MPDGQTSLLAKLIIIGAAIGLGRLMVSRDRITARLLIGRMIMGS
                     AVAPVAAIPLFK"
BASE COUNT           68 a           89 c           90 g           68 t
ORIGIN      
        1 tcggacgtgg tgaacatcag cgcaggtgaa gccggaaaga ccacgaaaaa aatcaagttc
       61 atggtcacaa gcccggactt tatcagcatt gacggcgtgc cctacctgtc agcccgtgac
      121 acccgcgatc tgaaggggtg atgccatgcc ggacggtcag acttcactcc tggcaaaact
      181 gattatcatc ggtgctgcca tcgggctggg gcgtctcatg gtgagccgcg atcgcatcac
      241 agcccgtctg cttatcgggc gaatgattat gggctctgcc gtggcaccgg ttgccgccat
      301 tccgctgttt aaatt
//
LOCUS       DAZFLD010000850          314 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.854, whole genome shotgun sequence.
ACCESSION   DAZFLD010000850 DAZFLD010000000
VERSION     DAZFLD010000850.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..314
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.854"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(107..>314)
                     /locus_tag="ACHKBL_005589"
     CDS             complement(107..>314)
                     /locus_tag="ACHKBL_005589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5822270.1"
                     /translation="KRLESSNREFIRYRFAQCSFALTWGARVGRLAFSRLYPCYTELN
                     KIRAMQLNNAYISDFQNPDEISSK"
BASE COUNT           95 a           46 c           64 g          109 t
ORIGIN      
        1 gaaaaataac ggtattgcgt gtaagtggtt tgatcatcga attcgtctga ttgtactgaa
       61 tatgtaattg tggccagtat aaatagtaat gttaatttta taataatcat ttgctggata
      121 tctcatctgg attttgaaaa tctgaaatgt aagcattgtt tagttgcatg gctcttattt
      181 tgtttaactc agtatagcat ggatatagtc gagagaatgc cagacgtcct actctggccc
      241 cccaggttag agcaaaagag cattgagcaa atctgtaacg tataaattcc cgattagaag
      301 attctagtct tttt
//
LOCUS       DAZFLD010000851          314 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.855, whole genome shotgun sequence.
ACCESSION   DAZFLD010000851 DAZFLD010000000
VERSION     DAZFLD010000851.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..314
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.855"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>314
                     /locus_tag="ACHKBL_005590"
     CDS             <1..>314
                     /locus_tag="ACHKBL_005590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="relaxase"
                     /protein_id="HFO5822271.1"
                     /translation="ELIIMLVRFSGHNNGAKEYLEEGKKNGRELTRDELDTRITLYGD
                     LELTQKIYQSIPDNDQERYVSITHSYFEDDISVDVMKDIFYEYKNFLMCAYRDDEFNI
                     YA"
BASE COUNT          106 a           32 c           68 g          108 t
ORIGIN      
        1 ggagttaata ataatgctcg ttaggttttc tggtcataat aatggagcaa aagaatatct
       61 tgaagagggt aaaaaaaatg ggcgagaatt gactcgtgat gagctggata ccagaattac
      121 tttatatggt gatttggaat taacccaaaa aatttatcag tctatacctg ataatgatca
      181 ggagcgttat gtttcaatta cacattcgta ttttgaggat gatatttctg ttgatgtgat
      241 gaaagatatt ttttatgagt ataaaaactt tcttatgtgc gcgtataggg atgatgaatt
      301 taatatatat gcag
//
LOCUS       DAZFLD010000852          314 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.856, whole genome shotgun sequence.
ACCESSION   DAZFLD010000852 DAZFLD010000000
VERSION     DAZFLD010000852.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..314
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.856"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>314)
                     /locus_tag="ACHKBL_005591"
     CDS             complement(<1..>314)
                     /locus_tag="ACHKBL_005591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004115529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer ATPase"
                     /protein_id="HFO5822272.1"
                     /translation="AMIREALLMATHTTYREGRQMLPVDLQSALWEISRDTQRNDVRR
                     AKAAEMAESLGMFTQPGSFEAELFNREGKLWPEADVTLIDLGHLAREGYEAQMALTMV
                     SMT"
BASE COUNT           65 a          110 c           72 g           67 t
ORIGIN      
        1 gtcattgaca ccatggtcag tgccatctgg gcttcataac cttcacgggc aagatgcccc
       61 agatcaatca gggtcacatc cgcttccggc cacagttttc cttcccggtt aaacagctcc
      121 gcttcaaaac tgcccggctg ggtgaacatc cccagcgact ctgccatctc agcagcttta
      181 gcccggcgaa catcattacg ctgagtgtcc cgcgaaattt cccacaacgc actctgcaga
      241 tccaccggca gcatctgacg cccctcccgg tacgtggtgt gcgtggccat cagcagtgcc
      301 tcacggatca tcgc
//
LOCUS       DAZFLD010000853          314 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.857, whole genome shotgun sequence.
ACCESSION   DAZFLD010000853 DAZFLD010000000
VERSION     DAZFLD010000853.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..314
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.857"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..238)
                     /locus_tag="ACHKBL_005592"
     CDS             complement(<1..238)
                     /locus_tag="ACHKBL_005592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000731266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1327 domain-containing protein"
                     /protein_id="HFO5822273.1"
                     /translation="MNKQYELVVKGINNYPDKITVTVALEIAGQPSLLSPYVAISLDR
                     TEGATLEFYEAEAKKKAKQFFMDIAAALCEGDEQS"
     gene            complement(242..>314)
                     /locus_tag="ACHKBL_005593"
     CDS             complement(242..>314)
                     /locus_tag="ACHKBL_005593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0001907 - symbiont-mediated killing of host
                     cell [Evidence IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HFO5822274.1"
                     /translation="LLTYLTNLYFKIKEDRRKAARGE"
BASE COUNT           76 a           90 c           57 g           91 t
ORIGIN      
        1 gtgactgttc atccccttca cataaagcgg cagcaatatc catgaaaaac tgcttcgcct
       61 tctttttcgc ctcagcttcg taaaattcca gcgtggcacc ttcagtacgg tcaagactaa
      121 tcgccacata tggcgacaac aacgacggct gcccggcaat ttccagtgcc acagtaacag
      181 taatcttatc cgggtaatta tttatccctt taacaaccag ttcgtattgt ttattcatcg
      241 tctactctcc ccgtgccgcc ttgcgccggt cctctttaat tttgaaatac aggtttgtca
      301 ggtacgtcag caac
//
LOCUS       DAZFLD010000854          313 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.858, whole genome shotgun sequence.
ACCESSION   DAZFLD010000854 DAZFLD010000000
VERSION     DAZFLD010000854.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..313
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.858"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..297)
                     /locus_tag="ACHKBL_005594"
     CDS             complement(<1..297)
                     /locus_tag="ACHKBL_005594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001423391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5822275.1"
                     /translation="MLLAVFIYLIRRNKLHKALRFYDESQKYELPFGYLPGAFSVLRI
                     ALEIKVNREKIKHGSLFELLDYVKAYQDAFMDLRVVTDPAYNEDEIQYDANNFTL"
BASE COUNT           99 a           69 c           54 g           91 t
ORIGIN      
        1 cagagtaaaa ttattcgcat catattgtat ttcatcttca ttatacgcag gatccgtcac
       61 gacccttaag tccataaatg catcctgata agcttttaca taatcaagta attcgaacag
      121 tgaaccatgc tttattttct ccctgtttac tttgatctcc agcgctatcc tgagaactga
      181 aaaagcgccg ggaagatacc caaaaggtaa ctcgtatttt tgggattcat cataaaaccg
      241 aagagctttg tgaagtttgt tccgccgaat cagataaata aaaacggcca gtaacatgta
      301 gcacctggtc aca
//
LOCUS       DAZFLD010000855          313 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.859, whole genome shotgun sequence.
ACCESSION   DAZFLD010000855 DAZFLD010000000
VERSION     DAZFLD010000855.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..313
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.859"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>313)
                     /locus_tag="ACHKBL_005595"
     CDS             complement(<1..>313)
                     /locus_tag="ACHKBL_005595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000062531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5822276.1"
                     /translation="LLEKNPGYYTQAEQQHAGMGFVSTDGIHWYVSADGNHRSCLARF
                     LFHLQGEGRTQLHNVAQSIYHTDQAFRSACREIHNLAEPLSRHDVYLRLQIRRQCVSR
                     E"
BASE COUNT           72 a           91 c           83 g           67 t
ORIGIN      
        1 tcttcgcggg aaacacactg ccgccggatc tgcagacgaa gatacacatc atgccgggac
       61 aacggttctg ccaggttgtg aatttcccga caggcagacc ggaacgcctg gtctgtgtga
      121 taaatactct gggccacatt gtgaagctgt gtgcgcccct ccccctgcag gtggaacaga
      181 aagcgggcca gacagctgcg gtgattaccg tcagcactga cataccagtg aatgccatcc
      241 gtgctgacaa aacccatccc ggcatgctgt tgctctgcct gcgtgtaata tcccggattc
      301 ttttcaagta acg
//
LOCUS       DAZFLD010000856          311 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.860, whole genome shotgun sequence.
ACCESSION   DAZFLD010000856 DAZFLD010000000
VERSION     DAZFLD010000856.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..311
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.860"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..182
                     /locus_tag="ACHKBL_005596"
     CDS             <1..182
                     /locus_tag="ACHKBL_005596"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5822277.1"
                     /translation="RLPEWPEERLHDLLPFSAYRFAELTDVTVIPPNAGKRVMEVVSS
                     TPMNDTASEVKIPTQ"
BASE COUNT          108 a           76 c           52 g           75 t
ORIGIN      
        1 cacgtctgcc tgaatggccg gaagagcgct tgcacgactt actaccgttc tcagcctacc
       61 gattcgcaga attaactgac gttacggtca taccacctaa tgcagggaaa cgagtcatgg
      121 aggtcgtcag ctctactccc atgaatgaca cagcctctga agtaaaaata ccgacgcagt
      181 gaaaccagcc ttaagctttc caaaacacaa ttacaacata gttgaacaaa gttcattcaa
      241 acaggttctt ttaataacat ttagtcaaat aaaataacaa taaaacgatt cagcaatcat
      301 ttttcataac a
//
LOCUS       DAZFLD010000857          307 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.861, whole genome shotgun sequence.
ACCESSION   DAZFLD010000857 DAZFLD010000000
VERSION     DAZFLD010000857.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..307
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.861"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(63..>307)
                     /locus_tag="ACHKBL_005597"
     CDS             complement(63..>307)
                     /locus_tag="ACHKBL_005597"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5822278.1"
                     /translation="SSWLAMSPSVKCDRYTFSGLVGAGTGKIFIQPDGTGDALFVVRL
                     SGEVVTPQCQILLKGIAADAFDPRGQFFNSISSFGE"
BASE COUNT           75 a           83 c           88 g           61 t
ORIGIN      
        1 gctgacctga ttgcgcgtta tccgggctgt gagatgcaga tcgtcgagaa gattaacgag
       61 gtttactccc cgaaactgga gattgagtta aaaaactgac cgcgcgggtc gaaagcatcc
      121 gccgcaatcc ccttgagcag tatctggcat tgcggcgtca ctacctcccc ggagagccgg
      181 acgacgaaga gagcatcgcc cgtgccatct ggctggatga atattttgcc cgtacccgcg
      241 ccaacaagac cgctgaaggt atagcgatcg catttaacgg aaggtgacat ggccagccag
      301 ctggatt
//
LOCUS       DAZFLD010000858          306 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.862, whole genome shotgun sequence.
ACCESSION   DAZFLD010000858 DAZFLD010000000
VERSION     DAZFLD010000858.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..306
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.862"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            23..>306
                     /locus_tag="ACHKBL_005598"
     CDS             23..>306
                     /locus_tag="ACHKBL_005598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     small subunit"
                     /protein_id="HFO5822279.1"
                     /translation="MSSSFQNEIPKARVNLKLDLHTGGGQKKVELPLKLLVAGDFSNG
                     QEERPLSERIKMDVNKNNFNAVLADISPSVRLNVENTLAGDGTEESISLT"
BASE COUNT           88 a           61 c           86 g           71 t
ORIGIN      
        1 gcaggtttca caggagaacg tcatgagcag ttcgtttcag aatgagatcc cgaaggcaag
       61 ggtgaatctg aaattagacc tacatacagg tggtgggcag aagaaagtgg agcttccgct
      121 caaactgctg gttgccggtg atttcagtaa cggacaggaa gagcgcccgc tgtcagagcg
      181 cataaaaatg gatgttaata aaaataactt taatgctgtt ctggctgata tctcgccgtc
      241 agtacgcctg aatgtggaga acacacttgc cggtgatggt actgaagaga gcatcagcct
      301 gacttt
//
LOCUS       DAZFLD010000859          306 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.863, whole genome shotgun sequence.
ACCESSION   DAZFLD010000859 DAZFLD010000000
VERSION     DAZFLD010000859.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..306
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.863"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            81..278
                     /locus_tag="ACHKBL_005599"
     CDS             81..278
                     /locus_tag="ACHKBL_005599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO5822280.1"
                     /translation="MSNNLLRLSDVMRRTGYSRAWIYRLINQGRFPKPVKIGSRSVAF
                     IESEVDEWINQRIDASRNNAA"
BASE COUNT           80 a           64 c           65 g           97 t
ORIGIN      
        1 ttaaaaatgg tatccaccta taaatatatt gtccttttat acatcattgt agacggttat
       61 agacgttacg aggattgaat atgtcaaata acttacttcg tctttctgat gttatgcgcc
      121 gtactggtta cagtcgcgct tggatttatc gcctgatcaa ccaggggcgc ttccctaaac
      181 ctgtaaaaat tggttcccgc tccgtggctt ttattgaaag cgaagtcgat gaatggatta
      241 accagcgtat tgatgcatct cgcaacaacg ccgcgtaata atcttttggg agggggatat
      301 tctccc
//
LOCUS       DAZFLD010000860          306 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.864, whole genome shotgun sequence.
ACCESSION   DAZFLD010000860 DAZFLD010000000
VERSION     DAZFLD010000860.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..306
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.864"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
BASE COUNT           85 a           54 c           85 g           82 t
ORIGIN      
        1 ttaagtttga acgcatttgc gggtggcagc ggctggaacc cagacagggt agacccgagc
       61 cgttgtgtta tggcgacggg gtcaggtcct acctctgcgg gttatatggt ataccgaacg
      121 actgactcag tgtgtatgca gggtataaat gaaggaaaag tgaaaggtgt ccatatttat
      181 gatgggcatc atgagttcaa tgatggtact gttgacacgt ttagcggtaa tgttacccca
      241 atatcacctt tgacattaaa aactgatgtt caaaaagtta ataaagttgg catcaaacgc
      301 tggtgg
//
LOCUS       DAZFLD010000861          303 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.865, whole genome shotgun sequence.
ACCESSION   DAZFLD010000861 DAZFLD010000000
VERSION     DAZFLD010000861.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..303
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.865"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>303
                     /locus_tag="ACHKBL_005600"
     CDS             <1..>303
                     /locus_tag="ACHKBL_005600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5822281.1"
                     /translation="KQAAQSAVDKLQAGNDKSGLQGRLDGLTPAAVPAVTDKDGNGKA
                     DTADKALADAEAAVRAAEAKKDAADDAAAKADKDGNKLITPAEAKAVTDANAELATA"
BASE COUNT           79 a           71 c          119 g           34 t
ORIGIN      
        1 aaacaggcgg cacagtcagc ggtggataaa ctgcaggcgg gtaacgacaa gagcggtctg
       61 cagggacgtc ttgatggtct gaccccggcg gcggttccgg cggtgacgga taaggacggc
      121 aacggtaagg cggacacggc agacaaggct ctggcggatg cggaagccgc agtccgggcg
      181 gcggaagcga agaaagatgc agcagatgat gctgcagcga aagcagacaa ggacggtaac
      241 aagctgatta ccccggcgga agcgaaggcg gtgacggatg cgaatgcgga gctggcgaca
      301 gcg
//
LOCUS       DAZFLD010000862          302 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.866, whole genome shotgun sequence.
ACCESSION   DAZFLD010000862 DAZFLD010000000
VERSION     DAZFLD010000862.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..302
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.866"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
BASE COUNT           98 a           61 c           50 g           93 t
ORIGIN      
        1 aatatattat cagaatccca tgttacgact tgctcgaggg ctatattatc ttgatagtcc
       61 atgccagcat tataataatc acaagatata attatcatgg aaataattat cactaatatc
      121 ttcccaagca tttccattta ctttacctct tgaaattcaa atgggaatcc agtgaaagga
      181 cctttaggca taatatatct ttgatatcca taaagctcac cgagcacttg taggcttata
      241 ggcgcatttg tattcataga ggggttgtta aacccaggga aagattacga aagcccgctc
      301 cc
//
LOCUS       DAZFLD010000863          301 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.867, whole genome shotgun sequence.
ACCESSION   DAZFLD010000863 DAZFLD010000000
VERSION     DAZFLD010000863.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..301
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.867"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            89..183
                     /locus_tag="ACHKBL_005601"
     tRNA            89..183
                     /locus_tag="ACHKBL_005601"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:123..125,aa:Sec,seq:tca)
BASE COUNT           67 a           84 c           75 g           75 t
ORIGIN      
        1 tattaggcgg aaaaaactga tctgggggat gtagaaactc aaggaagtag ctataatgcg
       61 ccccgcctcc atgtagcaat cgaggcgcgg aagatcgtcg tctccggtga ggcggctgga
      121 cttcaaatcc agttggggcc gccagcggtc ccgggcaggt tcgactcctg tgatcttccg
      181 ccaattaaca tcttctgaac tctcccgatg tcaatccccc ccttctaatc ccttgtatat
      241 caggattcct ttgtatccag aagtcaaccg acttcacccc gtatcaagtt gcttttgggg
      301 g
//
LOCUS       DAZFLD010000864          300 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.868, whole genome shotgun sequence.
ACCESSION   DAZFLD010000864 DAZFLD010000000
VERSION     DAZFLD010000864.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 300)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 300)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..300
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.868"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..201
                     /locus_tag="ACHKBL_005602"
     CDS             <1..201
                     /locus_tag="ACHKBL_005602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000786263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5822282.1"
                     /translation="GLPEYHIIPSVFDEEILCGINRNFGCQALKEAGILIHAGDKNWT
                     TKTIKVNGIQQRFIVLLDQPEE"
BASE COUNT          100 a           49 c           73 g           78 t
ORIGIN      
        1 ggattaccgg aataccacat cataccgtca gtatttgatg aagaaatcct gtgtggcatt
       61 aaccgaaatt ttggatgcca ggcactgaaa gaagccggaa ttctgattca tgcaggtgat
      121 aaaaactgga cgaccaaaac gataaaggtt aacggaatac agcaacgctt tattgtgctt
      181 cttgaccagc cggaggagta aaaaatcgta ttctcgtgta cgcgtaaaaa agtgggcgga
      241 taaggcggat aagcggatat ggttaataaa gtatttatat atcagtgcat tatgttgtat
//
LOCUS       DAZFLD010000865          296 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.869, whole genome shotgun sequence.
ACCESSION   DAZFLD010000865 DAZFLD010000000
VERSION     DAZFLD010000865.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..296
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.869"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>296
                     /locus_tag="ACHKBL_005603"
     CDS             <1..>296
                     /locus_tag="ACHKBL_005603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5822283.1"
                     /translation="VSLPRTGKGFTIGGRESRQAMGRASRAQSPSTQAIQDLSESLIR
                     HRKNEVGNAFLKLVQDNPDKDYWQVFTDDRPDTMRTIAERKDQETGETRREVVE"
BASE COUNT           77 a           82 c           87 g           50 t
ORIGIN      
        1 ggtgtcactt ccccgtacag gtaagggctt caccatcggc ggacgtgaaa gcaggcaggc
       61 catggggcgt gcatcccgtg cacagtctcc gtccactcag gcgatacagg acctgagcga
      121 atcgctgatc cgccatcgca aaaacgaagt gggtaacgcc ttcctgaaac tggtgcagga
      181 taatcccgac aaggattact ggcaggtatt caccgatgac agaccggata ccatgcggac
      241 gattgcagag cgcaaggacc aggaaactgg tgaaacccgt cgcgaagttg tcgaac
//
LOCUS       DAZFLD010000866          296 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.870, whole genome shotgun sequence.
ACCESSION   DAZFLD010000866 DAZFLD010000000
VERSION     DAZFLD010000866.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..296
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.870"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..272
                     /locus_tag="ACHKBL_005604"
     CDS             <1..272
                     /locus_tag="ACHKBL_005604"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5822284.1"
                     /translation="WGQKARKVIREVLQARKVLKVNEEKPDHRDRWEHEVSVGRLAPE
                     VNLVLQVREANEERPDLRDLVESQVRQAALQMWLMRRRHRRELCS"
BASE COUNT           76 a           69 c          116 g           35 t
ORIGIN      
        1 tatggggcca aaaggcgaga aaggtgatcc gggaggtcct gcaggcccgc aaggtcctaa
       61 aggtgaacga ggagaagccg gaccacaggg accgatggga gcacgaggtg agcgtgggga
      121 gactggcccc cgaggtgaac ctggtcctgc aggtccgaga ggcgaacgag gagagaccgg
      181 acctcaggga cctcgtggag agccaggtcc ggcaggcagc gctgcaaatg tggctgatgc
      241 gacgacggca cagaagggaa ttgtgcagtt aagcagcgca acggacagtg atgatg
//
LOCUS       DAZFLD010000867          295 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.871, whole genome shotgun sequence.
ACCESSION   DAZFLD010000867 DAZFLD010000000
VERSION     DAZFLD010000867.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..295
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.871"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>295
                     /locus_tag="ACHKBL_005605"
     CDS             <1..>295
                     /locus_tag="ACHKBL_005605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO5822285.1"
                     /translation="INAPAAPASIELTPGYYQITAVPHLTVYDPTVQFEFWFSEAKIA
                     DTSQVETSARYLGTGSQWSVSGPHIKPGKDFWFYVRSVNLVGKSAFVEASGRAS"
BASE COUNT           64 a           75 c           92 g           64 t
ORIGIN      
        1 attaacgcac cggcagcacc ggccagcatt gaactgacgc cggggtatta tcagataacg
       61 gcggtcccgc atcttacggt gtatgacccg acggtacagt ttgagttctg gttttcggag
      121 gcaaaaatcg cagacacatc tcaggtggaa acctctgccc gttatctggg gacaggcagc
      181 cagtggagtg tctccggccc gcacattaag ccggggaagg atttctggtt ttatgtgcgc
      241 agcgtcaacc tggtggggaa atctgcgttt gtggaagcca gtggccgggc cagca
//
LOCUS       DAZFLD010000868          294 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.872, whole genome shotgun sequence.
ACCESSION   DAZFLD010000868 DAZFLD010000000
VERSION     DAZFLD010000868.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..294
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.872"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            99..290
                     /locus_tag="ACHKBL_005606"
     CDS             99..290
                     /locus_tag="ACHKBL_005606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001186248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5822286.1"
                     /translation="MQQITLAEASLQAQQVEVISRMIERNHEEIDGHDLSVIAGLIKQ
                     LSSNVAVWLMTEEEKRGEQ"
BASE COUNT           91 a           59 c           78 g           66 t
ORIGIN      
        1 ggtagctggc tgattgctgc atcgggtgat gatgcaatat gcgtcaatga ctgaatcctt
       61 tgttgttctg aatatcatga aattaagggg gtgcagtaat gcagcaaata acactggcag
      121 aagcctcact gcaagcgcaa caggtcgaag taatcagccg catgattgaa cgcaaccacg
      181 aagaaataga cggccatgac ctttctgtta ttgcaggact tatcaaacaa ctttccagca
      241 atgtggcggt gtggttaatg acggaggaag aaaaacgcgg cgaacaataa cggc
//
LOCUS       DAZFLD010000869          290 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.873, whole genome shotgun sequence.
ACCESSION   DAZFLD010000869 DAZFLD010000000
VERSION     DAZFLD010000869.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..290
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.873"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>290)
                     /locus_tag="ACHKBL_005607"
     CDS             complement(<1..>290)
                     /locus_tag="ACHKBL_005607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016191725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer ATPase"
                     /protein_id="HFO5822287.1"
                     /translation="ERDQYLGRDILFVVDEAHIVTVNPLLSPYMTKVVKMWRKLGAWL
                     WLATQNLKDYPDVSEKMLNMAEWWVCLTMPPEEVNNIARFKSLTEEQKAVLLS"
BASE COUNT           66 a           99 c           60 g           65 t
ORIGIN      
        1 gacagcagta ccgccttctg ctcctccgtc agggatttaa aacgggcaat attgttcact
       61 tcctccggcg gcatggtcag gcacacccac cactctgcca tattcagcat cttctccgag
      121 acatccggat aatccttcag gttctgcgtg gccagccaca gccaggcccc cagcttgcgc
      181 cacattttca ccactttggt catatacggg gacaaaagcg ggtttaccgt cacgatatgc
      241 gcctcatcca ccacaaacag aatatcccgt cccagatact gatcgcgctc
//
LOCUS       DAZFLD010000870          289 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.874, whole genome shotgun sequence.
ACCESSION   DAZFLD010000870 DAZFLD010000000
VERSION     DAZFLD010000870.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..289
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.874"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>289
                     /locus_tag="ACHKBL_005608"
     CDS             <1..>289
                     /locus_tag="ACHKBL_005608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pilus assembly protein PilL"
                     /protein_id="HFO5822288.1"
                     /translation="DAAMPVPSVTVNRNVQPASPDVYSQAPEVVRYDRYLLVSTEPQA
                     VQRDPLSQVIDIRIPSSLKPTVADAMRYALKQSGYTLCATGPANGVLYRQPL"
BASE COUNT           54 a           95 c           80 g           60 t
ORIGIN      
        1 gggatgcggc catgccggta ccgtcggtca cagtaaaccg taatgtacag cctgccagcc
       61 cggatgtgta ttctcaggca ccggaggtgg tgcgctatga ccgttacctg ctggtcagca
      121 cagagccgca ggctgtacag cgtgatccac tttctcaggt gatcgacatt cgcattccct
      181 cctccctgaa acccacggtg gccgatgcca tgcgttatgc cctgaaacag tccggttaca
      241 cgctctgcgc caccggaccg gccaacggcg tgctgtaccg tcagcctct
//
LOCUS       DAZFLD010000871          288 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.875, whole genome shotgun sequence.
ACCESSION   DAZFLD010000871 DAZFLD010000000
VERSION     DAZFLD010000871.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..288
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.875"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(167..280)
                     /locus_tag="ACHKBL_005609"
     CDS             complement(167..280)
                     /locus_tag="ACHKBL_005609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFO5822289.1"
                     /translation="MICITVVLIIWITHSSLCEFRFRIAGAEIAAFLQCKQ"
BASE COUNT           66 a           85 c           69 g           68 t
ORIGIN      
        1 cacacccgat attctgactg tcatggattt cccccataaa aaaaccgcag taccggtttc
       61 ccggcctgcg gtaaaatttg tggtttgttg gtgttaaaac ggtgccatcc ggctgaccac
      121 cctcagcaac cggtcggcgg gggatattct cccccgccac ggtttcttac tgcttacact
      181 gtaagaacgc cgcaatctcc gcgcccgcta tccggaaccg gaactcgcac agtgaactgt
      241 gggtgatcca gataatgagc actaccgtga tacaaatcac ggtggttt
//
LOCUS       DAZFLD010000872          285 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.876, whole genome shotgun sequence.
ACCESSION   DAZFLD010000872 DAZFLD010000000
VERSION     DAZFLD010000872.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..285
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.876"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
BASE COUNT           86 a           63 c           52 g           84 t
ORIGIN      
        1 actcaatcca ttttacccag agtcataaaa tagagtgtct gttgatctca ttttcatatt
       61 cctttcagac tgaagtgcgt tttcatcacc cgatgagaaa gaactgaagt gatgtttgcc
      121 atagattgcg agatgccctg tctcattgac tatcgcggtg cacctttata cctgttatac
      181 cagatcaaaa atcacgcaat ccatacaaca aaacgagatt tgcaattcgt gtcacaaaat
      241 atgtcgatct ttttctaaga ggcagatgcc atgtgaagcc agacg
//
LOCUS       DAZFLD010000873          285 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.877, whole genome shotgun sequence.
ACCESSION   DAZFLD010000873 DAZFLD010000000
VERSION     DAZFLD010000873.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..285
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.877"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
BASE COUNT           77 a           74 c           43 g           91 t
ORIGIN      
        1 ttaatatatt aatactcata ttcgactcct gtgatcgcca aaatgcctct tcagtgatcc
       61 ccatcctgct tatttctgag caacctgacc ctgctgatgt atctggtagt atgacagagt
      121 gattcatttt tcttgccgag tgtacgaaaa cgtcttcctt gccaccgact atcaaccgtc
      181 actcaaaaaa acctctcttt ttttcaagct atcaagaata agttgcattt tatctgcacc
      241 ggacaccagc catccatata tggcttttac ctgctacaaa gatga
//
LOCUS       DAZFLD010000874          284 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.878, whole genome shotgun sequence.
ACCESSION   DAZFLD010000874 DAZFLD010000000
VERSION     DAZFLD010000874.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..284
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.878"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..264
                     /locus_tag="ACHKBL_005610"
     CDS             <1..264
                     /locus_tag="ACHKBL_005610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFO5822290.1"
                     /translation="AAAEAGSKAAWERARTASQAWSQRENDVASSLQSCLSRIKDVSI
                     TLPQFPSLDKILRDLENQVCDAVVDRVNEHLPGNIDPWKDYNL"
BASE COUNT           70 a           92 c           77 g           45 t
ORIGIN      
        1 gctgccgcag aagccggcag caaagccgcc tgggagcggg cgcgcacagc cagccaggca
       61 tggtcacagc gagaaaacga cgtcgcctcc tccctgcagt cctgcctgtc ccgcattaag
      121 gacgtcagca tcactcttcc acaattcccc agcctggaca aaatccttcg cgatctggaa
      181 aaccaggtct gtgatgccgt cgtggatcgg gtgaatgaac acctgccggg gaatatcgac
      241 ccctggaaag actataacct ctgaacagga gccccatgag cgaa
//
LOCUS       DAZFLD010000875          284 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.879, whole genome shotgun sequence.
ACCESSION   DAZFLD010000875 DAZFLD010000000
VERSION     DAZFLD010000875.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..284
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.879"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(113..>284)
                     /locus_tag="ACHKBL_005611"
     CDS             complement(113..>284)
                     /locus_tag="ACHKBL_005611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000670135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type II secretion protein F"
                     /protein_id="HFO5822291.1"
                     /translation="DSVSAMNTYAGQWLEDAKKSVRKSGLIITGISGGFVFFFILNMI
                     SAIYSISDMLKQ"
BASE COUNT           87 a           50 c           55 g           92 t
ORIGIN      
        1 taaagaaaaa taagaagaca gacgtgctct gaagttcata tatgactcct gtattaatgg
       61 ttgttacgtt aatgaaagta tattgaaggg cgttttagtg ttacggcagg tattattgct
      121 tgagcatatc gctgatactg tatatcgcgc taatcatgtt aagaataaaa aagaatacaa
      181 aacctcctga aataccggtg ataattaatc cactttttct gacgctcttt tttgcatctt
      241 ccagccactg tccggcatag gtattcattg cagatacgct gtct
//
LOCUS       DAZFLD010000876          283 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.880, whole genome shotgun sequence.
ACCESSION   DAZFLD010000876 DAZFLD010000000
VERSION     DAZFLD010000876.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..283
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.880"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
BASE COUNT           91 a           76 c           61 g           55 t
ORIGIN      
        1 atgcgcgagt ttgtgcgaaa aacattcaca tatccttccc gaaaggacgg ataagaacca
       61 ccagcacggt cgcttaaggt acgacggcgc attgctttac agaggtacac aatgaccaac
      121 gacgaactac agaaaatcgc attatccatt attgagcggc agcgcgaaaa agcactccag
      181 gaacgtagat tgcgacttaa tcccccaccg ctcaccagcc aaacacaatt tgcgcttgag
      241 gcttttgtag agaaataccc acggcggctt aaatccgcta aaa
//
LOCUS       DAZFLD010000877          281 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.881, whole genome shotgun sequence.
ACCESSION   DAZFLD010000877 DAZFLD010000000
VERSION     DAZFLD010000877.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..281
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.881"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>281
                     /locus_tag="ACHKBL_005612"
     CDS             <1..>281
                     /locus_tag="ACHKBL_005612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001497294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFO5822292.1"
                     /translation="DTIGPYYVVGPGIAMPHARPEEGANKLSLALTLIPSGVNFDADE
                     NDPVKLLIVLAATDSTSHIEAISQLAKLFDNEKDIQAILTAKTTQDILS"
BASE COUNT           75 a           70 c           68 g           68 t
ORIGIN      
        1 acgacactat cggtccgtac tatgtggttg gccctggtat tgcgatgcct catgcgcgcc
       61 cggaagaggg ggctaacaag ctgtcattag cactgaccct gatcccttca ggtgtgaatt
      121 ttgatgccga tgaaaacgat ccagtgaaat tactgattgt gctggctgca accgacagta
      181 ccagccacat tgaagcgatt tctcaactcg ccaagttatt cgataacgaa aaagacatac
      241 aggccatttt gacggccaaa acgacgcagg acattttgtc t
//
LOCUS       DAZFLD010000878          279 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.882, whole genome shotgun sequence.
ACCESSION   DAZFLD010000878 DAZFLD010000000
VERSION     DAZFLD010000878.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 279)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 279)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..279
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.882"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
BASE COUNT           75 a           96 c           58 g           50 t
ORIGIN      
        1 acagctgcat cgtcgcattc cagacgaacc tgcaccctgt cagcccgacc gtttacagta
       61 aatacctgca ctgaacttat gcgatagttc agcatataca ctctccctct cagctcctca
      121 ggaatacccc ggaaccgtca gtcatgccac cggggagaaa agcaggcagc gaatcatgac
      181 cgcatacaga acgctgcccg ccccggtgac tcagacccgg gttcagaact cccagttgac
      241 acccacattc caggaaacat caccgtcact gaacgcagt
//
LOCUS       DAZFLD010000879          273 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.883, whole genome shotgun sequence.
ACCESSION   DAZFLD010000879 DAZFLD010000000
VERSION     DAZFLD010000879.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 273)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..273
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.883"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..230)
                     /locus_tag="ACHKBL_005613"
     CDS             complement(<1..230)
                     /locus_tag="ACHKBL_005613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO5822293.1"
                     /translation="MAKYSDEIKEAARSLYLKRWHPRDIAAELGIPPRTVYHWCDVGQ
                     WATLLPAESVEDVIARRIDVLTGREGKTECELA"
BASE COUNT           57 a           92 c           72 g           52 t
ORIGIN      
        1 gcaagttcac actctgtctt gccctcccgt ccggtcagca catcaatacg ccgggcgatc
       61 acgtcctcaa cggattcagc gggcagcagg gtagcccact gccccacatc acaccagtgg
      121 taaaccgtgc gcggcggaat accgagttcc gccgcaatat cgcgggggtg ccaccgcttc
      181 aggtaaagcg atcgcgccgc ctctttgatt tcgtcagaat atttagccat gcgcccatta
      241 tggcggcgca tccggcccgc attcaggcag aaa
//
LOCUS       DAZFLD010000880          271 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.884, whole genome shotgun sequence.
ACCESSION   DAZFLD010000880 DAZFLD010000000
VERSION     DAZFLD010000880.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..271
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.884"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            113..271
                     /locus_tag="ACHKBL_005614"
                     /pseudo
     CDS             113..271
                     /locus_tag="ACHKBL_005614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309678.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
BASE COUNT           82 a           56 c           72 g           61 t
ORIGIN      
        1 atgaattaca aggggttaaa tgctgccgca gcatagcgat attgaaatag cctggtatgc
       61 ttcaatacag caggagccga atagctggaa gaccgtcacc acacagttct acatccagga
      121 attcagtgag tatattgcgc cactgcagga tgctgtagat ctggaaatcg caacggagga
      181 agaaagatcg ttgctggaag cctgaaaaaa gtatcgggtg ctgctaaacc gtgtggacac
      241 ttccgtagca ccagatatcg agtggcttat t
//
LOCUS       DAZFLD010000881          271 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.885, whole genome shotgun sequence.
ACCESSION   DAZFLD010000881 DAZFLD010000000
VERSION     DAZFLD010000881.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..271
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.885"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>271)
                     /locus_tag="ACHKBL_005615"
     CDS             complement(<1..>271)
                     /locus_tag="ACHKBL_005615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="very short patch repair endonuclease"
                     /protein_id="HFO5822294.1"
                     /translation="NTKLELRIRKPLFARGFRYRLHNSGLPGRPDMYFPKYNAVIFIH
                     GCFWHGHDNGCPISHTPKNNESYWQNKINKNQERDVKNQYVLASK"
BASE COUNT           79 a           49 c           48 g           95 t
ORIGIN      
        1 tttttagatg caagaacata ttgatttttg acatctcttt cttgattttt attgatttta
       61 ttttgccagt acgattcatt atttttagga gtgtgagata tagggcagcc gttatcatgc
      121 ccatgccaaa aacagccatg gataaaaatg actgcattgt atttagggaa atacatatct
      181 ggtctaccag gcaaaccact attatgcaat cgataacgaa accctctggc aaacaacggc
      241 ttcctgattc ttaactcaag tttggtgttt t
//
LOCUS       DAZFLD010000882          266 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.887, whole genome shotgun sequence.
ACCESSION   DAZFLD010000882 DAZFLD010000000
VERSION     DAZFLD010000882.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..266
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.887"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>266)
                     /locus_tag="ACHKBL_005616"
     CDS             complement(<1..>266)
                     /locus_tag="ACHKBL_005616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase family protein"
                     /protein_id="HFO5822295.1"
                     /translation="CRENRHRERRFILKSRQIGMTWYFAWEAFEDAVVTGDNQIFFSA
                     SRVQAEIFREYIINFALQFGITLTGKHIRLSNGAMLRFLSTNAST"
BASE COUNT           67 a           73 c           70 g           56 t
ORIGIN      
        1 gtactggcat tggtggacag gaaacgcagc attgcaccat tgctcagacg gatatgctta
       61 cccgtgagcg tgatcccgaa ctgcagggcg aagttaataa tatattcacg gaagatttcc
      121 gcctgtaccc gtgacgcaga aaagaaaatc tggttatcac cggtcaccac cgcatcttcg
      181 aacgcctccc atgcaaaata ccaggtcatg ccgatctggc ggctcttgag gatgaaacgg
      241 cgctcacggt gccggttctc gcggca
//
LOCUS       DAZFLD010000883          264 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.888, whole genome shotgun sequence.
ACCESSION   DAZFLD010000883 DAZFLD010000000
VERSION     DAZFLD010000883.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..264
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.888"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
BASE COUNT           79 a           65 c           35 g           85 t
ORIGIN      
        1 tcaggatatt ccgaaaataa ccccaaaata ttaatttaat gttttaggat ttgtcttaca
       61 ttattcttgg tgtagtcact cagaaccacg actacaccac cggaacaggc atcagaaaaa
      121 acttactctc atcaggcatt ctgtttcaca tctgcttaca ctgtaatttc tgatcaatat
      181 cacaacatta tccgaattta actgttcagt tcatctacaa tatttccctg cgtgacttta
      241 tatcagcccc gcacttccgt acgg
//
LOCUS       DAZFLD010000884          263 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.889, whole genome shotgun sequence.
ACCESSION   DAZFLD010000884 DAZFLD010000000
VERSION     DAZFLD010000884.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..263
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.889"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>263)
                     /locus_tag="ACHKBL_005617"
     CDS             complement(<1..>263)
                     /locus_tag="ACHKBL_005617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HFO5822296.1"
                     /translation="RAYDERDRLVSETSRSGDVIRYAYDNPHSELPATTTDATGSTRQ
                     MTWSRYGQLLAFTDCSGYQTRYEYDRFGQMTAVHREEGVSLYR"
BASE COUNT           47 a           87 c           74 g           55 t
ORIGIN      
        1 gcggtaaaga ctgacacctt cctcacggtg gaccgccgtc atctggccga agcggtcgta
       61 ttcatagcgg gtctggtagc ccgagcagtc ggtgaacgcc agcaactgac cgtagcggct
      121 ccacgtcatc tgccgggtgc tgcccgtcgc atctgttgtc gtggccggta actcactgtg
      181 cggattatca taagcatacc ggatgacgtc cccactgcgc gatgtctccg ataccagcct
      241 gtcccgctca tcatacgccc ggc
//
LOCUS       DAZFLD010000885          263 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.890, whole genome shotgun sequence.
ACCESSION   DAZFLD010000885 DAZFLD010000000
VERSION     DAZFLD010000885.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..263
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.890"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>263
                     /locus_tag="ACHKBL_005618"
     CDS             <1..>263
                     /locus_tag="ACHKBL_005618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFO5822297.1"
                     /translation="WLREKMKTLSRHSELAKAFAYALNQWPALTYYANDGWVEIDNNI
                     AENALRAVSLGRKNFLFFGSDHGGERGALLYSLIGTCKLNDVDP"
BASE COUNT           61 a           64 c           83 g           55 t
ORIGIN      
        1 tggttgcgtg aaaagatgaa gaccctgtcg cgacactcag agttggcgaa ggcgttcgcg
       61 tacgcactta accagtggcc ggcactgacg tactatgcga acgatggctg ggtggaaatc
      121 gacaacaaca tcgctgaaaa tgccctgcgg gcggtcagtc tgggtcgtaa aaacttcctg
      181 ttcttcggct ctgaccatgg tggtgagcgg ggagcgctac tgtacagcct gatcgggacg
      241 tgcaaactga atgacgtgga tcc
//
LOCUS       DAZFLD010000886          261 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.891, whole genome shotgun sequence.
ACCESSION   DAZFLD010000886 DAZFLD010000000
VERSION     DAZFLD010000886.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..261
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.891"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..237)
                     /locus_tag="ACHKBL_005619"
     CDS             complement(<1..237)
                     /locus_tag="ACHKBL_005619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZinT/AdcA family metal-binding protein"
                     /protein_id="HFO5822298.1"
                     /translation="MAIRLHKLAVALGVFIVSAPAFSHGHHSHGKPLTEVEQKAANGV
                     FDDANVQNRTLSDWDGVWQSVYPLLQSGKLDPVFQ"
BASE COUNT           80 a           58 c           58 g           65 t
ORIGIN      
        1 ctgaaagacg gggtcaagtt tgccactttg caataaagga taaacggatt gccagactcc
       61 atcccagtca cttagcgttc ggttttgtac attggcatca tcaaaaacac cattagcagc
      121 tttttgttcg acctctgtta agggtttgcc gtgtgagtga tgaccatgcg aaaaggcagg
      181 agcgctaaca ataaagacac ctaaagcaac agccagttta tgaagacgaa tcgccaaaac
      241 agtttcctcc agagttaatg t
//
LOCUS       DAZFLD010000887          258 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.892, whole genome shotgun sequence.
ACCESSION   DAZFLD010000887 DAZFLD010000000
VERSION     DAZFLD010000887.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..258
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.892"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            35..>258
                     /locus_tag="ACHKBL_005620"
     CDS             35..>258
                     /locus_tag="ACHKBL_005620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5822299.1"
                     /translation="MKGKPDVDFNQVFRESGMPETEEEIATLFHEDVQKTGLITNTSP
                     MSPFWRLILTIVIRPVQWLRDALVDVVLPG"
BASE COUNT           66 a           65 c           67 g           60 t
ORIGIN      
        1 caggattttg gacgcataaa caccagggtg aactatgaaa gggaaaccgg acgttgattt
       61 taatcaggta ttccgtgaaa gcggtatgcc ggaaacagaa gaagagatcg ccaccctctt
      121 tcatgaggat gtacagaaaa ccggactcat caccaacacc tcgcccatga gtcccttctg
      181 gcggctcatt ctgaccattg tcatacgacc cgttcagtgg ctgcgtgatg cgctggttga
      241 cgttgtgctg cccggtat
//
LOCUS       DAZFLD010000888          258 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.893, whole genome shotgun sequence.
ACCESSION   DAZFLD010000888 DAZFLD010000000
VERSION     DAZFLD010000888.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..258
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.893"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..254
                     /locus_tag="ACHKBL_005621"
     CDS             <1..254
                     /locus_tag="ACHKBL_005621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001506259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="integrating conjugative element protein"
                     /protein_id="HFO5822300.1"
                     /translation="TGMAEPYAAAQPAALEEGERRIEALDREIMALKNEMELKRDLAR
                     NSVLTIIERDNERVGNNPMIQQADNADSRIHSLDAPDKE"
BASE COUNT           64 a           58 c           88 g           48 t
ORIGIN      
        1 tcacggggat ggccgagccg tatgctgcag ctcagcctgc tgcactggaa gagggggaac
       61 gccgtattga ggcgctggat cgggaaatta tggcgctgaa aaacgagatg gagctgaagc
      121 gggatctggc gcgtaacagt gttctgacca tcattgaacg ggataacgag cgtgtgggga
      181 ataacccgat gatccagcag gctgacaatg ctgacagccg tatccattct ctcgatgccc
      241 cggataagga gtgagagg
//
LOCUS       DAZFLD010000889          256 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.894, whole genome shotgun sequence.
ACCESSION   DAZFLD010000889 DAZFLD010000000
VERSION     DAZFLD010000889.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 256)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 256)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..256
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.894"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
BASE COUNT           66 a           71 c           68 g           51 t
ORIGIN      
        1 cgtcgcgcgt ctttctgggg gtaacaaacg aagaattttc acacatcctg gcacgccaga
       61 tgaacgcaat gggctacggc ctggacgttg acgcacagga tatcagataa ggagtcacac
      121 cgtgagctgg ccgaatgtaa ccgtaagcca tcagaaccgt ttcaacggtg ccacccgtga
      181 agtggagcgc acgctgcttt ttgtgggcta tggcaaaaag aacacaggta acaccctttc
      241 cgtctcccct gagacc
//
LOCUS       DAZFLD010000890          255 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.895, whole genome shotgun sequence.
ACCESSION   DAZFLD010000890 DAZFLD010000000
VERSION     DAZFLD010000890.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..255
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.895"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            146..>255
                     /locus_tag="ACHKBL_005622"
     CDS             146..>255
                     /locus_tag="ACHKBL_005622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5822301.1"
                     /translation="MEKLTTELHSLSKMGRRHVISILAEIANGYDDFSDM"
BASE COUNT           92 a           44 c           41 g           78 t
ORIGIN      
        1 ttagtgattt ctcttcattc ttaaacgaaa tcatggacag ttgttcataa taatcaaaag
       61 gtaacaaaaa tcattgtaaa ccatgccatc tttttagaga ttaccaacag aaaattcctt
      121 gttcgaaact tagcaaatgc aagctatgga aaaacttaca acggaactac actccttaag
      181 taagatgggc cgaagacatg taatctctat tttagccgaa atagctaatg gctatgatga
      241 ttttagtgac atgtt
//
LOCUS       DAZFLD010000891          254 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.896, whole genome shotgun sequence.
ACCESSION   DAZFLD010000891 DAZFLD010000000
VERSION     DAZFLD010000891.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.896"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            157..>254
                     /locus_tag="ACHKBL_005623"
     CDS             157..>254
                     /locus_tag="ACHKBL_005623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFO5822302.1"
                     /translation="MSEDVPLPKANQRYRDDHGALVTVTSVEETRVV"
BASE COUNT           97 a           47 c           57 g           53 t
ORIGIN      
        1 catgaaagag gagagcaata aaagacacgg aaaataaaga agagagagaa aatatttctt
       61 aaaaacagaa gattaacaaa acacatcagg aggcagagac tcctgattca gcaatatcac
      121 acgaaatttt agttaactgc taccgttctg aaatctatgt ctgaagatgt tccattaccg
      181 aaagccaatc agcgatacag agacgatcat ggtgcgcttg tcaccgtgac cagtgttgag
      241 gaaacccggg tggt
//
LOCUS       DAZFLD010000892          253 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.897, whole genome shotgun sequence.
ACCESSION   DAZFLD010000892 DAZFLD010000000
VERSION     DAZFLD010000892.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.897"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>253)
                     /locus_tag="ACHKBL_005624"
     CDS             complement(<1..>253)
                     /locus_tag="ACHKBL_005624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5822303.1"
                     /translation="QRFSKLLPFRIDFAYRKDTPSFGHSCKHSMCMEIYRLLSETQTM
                     LAGYYWVMEYTPDKGLHIHFIGYLDGQRHKNSYQISRQLG"
BASE COUNT           73 a           52 c           52 g           76 t
ORIGIN      
        1 cccagttgcc tggatatctg ataagagttc ttgtgacgtt gtccatccag atagccgata
       61 aaatgaatat gaagcccttt gtctggtgta tattccatta cccagtaata tcctgctaac
      121 atcgtctggg tttcgctaag taaacggtat atttccatac acatactgtg cttacaggaa
      181 tgcccgaaac tgggcgtgtc tttcctgtag gcaaaatcaa ttctgaaagg taacagttta
      241 gaaaaacgtt gaa
//
LOCUS       DAZFLD010000893          251 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.denovo.898, whole genome shotgun sequence.
ACCESSION   DAZFLD010000893 DAZFLD010000000
VERSION     DAZFLD010000893.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..251
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.denovo.898"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..240
                     /locus_tag="ACHKBL_005625"
     CDS             <1..240
                     /locus_tag="ACHKBL_005625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5822304.1"
                     /translation="PGADGHIFPLSENGSFTPVGVFAPMSEKTSGKRPEGQSHSAGES
                     MLLKWTPPQIVNSKDDASPEAVVRFMKETDKQEKE"
BASE COUNT           73 a           65 c           68 g           45 t
ORIGIN      
        1 ccgggggctg atgggcacat tttccccctt tcagagaacg gttccttcac gcccgtcggc
       61 gttttcgccc ccatgtcaga aaaaacaagc ggtaagcgac cggaagggca atcccactca
      121 gcaggggaaa gtatgctgct gaaatggacg ccaccacaaa tcgtcaacag taaggacgac
      181 gcttctccgg aagcggtcgt gaggtttatg aaagagactg acaaacagga aaaagaatga
      241 gttcacctga a
//
LOCUS       DAZFLD010000894        17878 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.guided.096, whole genome shotgun sequence.
ACCESSION   DAZFLD010000894 DAZFLD010000000
VERSION     DAZFLD010000894.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17878)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17878
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.guided.096"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(251..796)
                     /gene="orn"
                     /locus_tag="ACHKBL_005626"
     CDS             complement(251..796)
                     /gene="orn"
                     /locus_tag="ACHKBL_005626"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HFO5822305.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEAYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
     gene            891..1943
                     /gene="rsgA"
                     /locus_tag="ACHKBL_005627"
     CDS             891..1943
                     /gene="rsgA"
                     /locus_tag="ACHKBL_005627"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HFO5822306.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            2040..3008
                     /gene="asd"
                     /locus_tag="ACHKBL_005628"
     CDS             2040..3008
                     /gene="asd"
                     /locus_tag="ACHKBL_005628"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaetidylserine decarboxylase"
                     /protein_id="HFO5822307.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLPAEEIEAEHDANPLVDDNKDQV"
     gene            3030..6353
                     /gene="mscM"
                     /locus_tag="ACHKBL_005629"
     CDS             3030..6353
                     /gene="mscM"
                     /locus_tag="ACHKBL_005629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HFO5822308.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQISQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQEVIDNYPKLSATLRAQLNNMRDEPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNSPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMDLVASQQRQAASQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNGQLEDALKEVNEATHRYLFWTSDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLLGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIVPLIMALMMFDNLDDRE
                     FSGSLGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVA
                     ILASAVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAE
                     MLAQRARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLW
                     SEIHSAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLE
                     LAILQHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFG
                     LQEIFANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNK
                     AFITEQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFL
                     VDLQQGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNG
                     KQTGRTLTSAGKGRQAGSL"
     gene            complement(6384..6698)
                     /locus_tag="ACHKBL_005630"
     CDS             complement(6384..6698)
                     /locus_tag="ACHKBL_005630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeO family protein"
                     /protein_id="HFO5822309.1"
                     /translation="MSARMFILCCIWFIVAFLWITITSALDKEWMIDGRGINNVCDVL
                     MYLEDDDTRDVGVIMTLPLFFPFLWFALWRKKRGWFMYATALAIFGYWLWQFFLRYQF
                     CL"
     gene            complement(6695..7009)
                     /gene="yjeN"
                     /locus_tag="ACHKBL_005631"
     CDS             complement(6695..7009)
                     /gene="yjeN"
                     /locus_tag="ACHKBL_005631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000340721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjeN"
                     /protein_id="HFO5822310.1"
                     /translation="MDDASRDPVITEDEIRELQFSAGDVAEIEQTVLSFVDTRHTRKV
                     AMVVGNTINTLKERDGPRWGNLPDIYCAYLIRCLVFRGELVGYGDLFRMRYSEIKRPI
                     IS"
     gene            complement(7072..8574)
                     /gene="yjeM"
                     /locus_tag="ACHKBL_005632"
     CDS             complement(7072..8574)
                     /gene="yjeM"
                     /locus_tag="ACHKBL_005632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
                     /protein_id="HFO5822311.1"
                     /translation="MPHTIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAIPFYIF
                     SALLFFIPFALMMAEMGAAYRKEEGGIYSWMNNSVGPRFAFIGTFMWFSSYIIWMVST
                     SAKVWVPFSTFLYGSDMTQHWRIAGLEPTQVVGLLAVAWMILVTVVASKGINKIARIT
                     AVGGIAVMCLNLVLLLVSITILLLNGGHFAQDINFLASPNPGYQSGLAMLSFVVFAIF
                     AYGGIEAVGGLVDKTENPEKNFAKGIVFAAIVISIGYSLAIFLWGVSTNWQQVLSNGS
                     VNLGNITYVLMKSLGVTLGNALHLSPEASLSLGVWFARITGLSMFLAYTGAFFTLCYS
                     PLKAIIQGTPKALWPEPMTRLNAMGMPSIAMWMQCGLVTVFILLVSFGGGTASAFFNK
                     LTLMANVSMTLPYLFLALAFPFFKARQDLDRPFVIFKTHMSAMIATVVVVLVVTFANV
                     FTIIQPVVEAGDWDSTLWMIGGPVFFSLLAMAIYQNYCSRMANKPELALD"
     gene            complement(8793..9770)
                     /gene="epmA"
                     /locus_tag="ACHKBL_005633"
     CDS             complement(8793..9770)
                     /gene="epmA"
                     /locus_tag="ACHKBL_005633"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HFO5822312.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            10095..11903
                     /gene="frdA"
                     /locus_tag="ACHKBL_005634"
     CDS             10095..11903
                     /gene="frdA"
                     /locus_tag="ACHKBL_005634"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HFO5822313.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAVIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            11896..12630
                     /gene="frdB"
                     /locus_tag="ACHKBL_005635"
     CDS             11896..12630
                     /gene="frdB"
                     /locus_tag="ACHKBL_005635"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HFO5822314.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            12641..13036
                     /gene="frdC"
                     /locus_tag="ACHKBL_005636"
     CDS             12641..13036
                     /gene="frdC"
                     /locus_tag="ACHKBL_005636"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HFO5822315.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            13047..13406
                     /gene="frdD"
                     /locus_tag="ACHKBL_005637"
     CDS             13047..13406
                     /gene="frdD"
                     /locus_tag="ACHKBL_005637"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HFO5822316.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            13469..14602
                     /locus_tag="ACHKBL_005638"
     CDS             13469..14602
                     /locus_tag="ACHKBL_005638"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HFO5822317.1"
                     /translation="MFKMTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGI
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDDVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANASIGLFGALAVKPSGLSFEQAMQTRVFQPLKLTHTWINVPSAEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLKPLDITEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDIIINGSDNKIALAARPVKAITPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAACQILNALQ"
     gene            14691..15224
                     /gene="blc"
                     /locus_tag="ACHKBL_005639"
     CDS             14691..15224
                     /gene="blc"
                     /locus_tag="ACHKBL_005639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HFO5822318.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDREMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(15225..15542)
                     /gene="sugE"
                     /locus_tag="ACHKBL_005640"
     CDS             complement(15225..15542)
                     /gene="sugE"
                     /locus_tag="ACHKBL_005640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HFO5822319.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(15717..15863)
                     /gene="ecnB"
                     /locus_tag="ACHKBL_005641"
     CDS             complement(15717..15863)
                     /gene="ecnB"
                     /locus_tag="ACHKBL_005641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HFO5822320.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(15974..16099)
                     /gene="ecnA"
                     /locus_tag="ACHKBL_005642"
     CDS             complement(15974..16099)
                     /gene="ecnA"
                     /locus_tag="ACHKBL_005642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HFO5822321.1"
                     /translation="MMKRLIVLVLLASALLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            complement(16151..16717)
                     /gene="efp"
                     /locus_tag="ACHKBL_005643"
     CDS             complement(16151..16717)
                     /gene="efp"
                     /locus_tag="ACHKBL_005643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFO5822322.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            16759..17787
                     /gene="epmB"
                     /locus_tag="ACHKBL_005644"
     CDS             16759..17787
                     /gene="epmB"
                     /locus_tag="ACHKBL_005644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0072581 - protein-N6-(L-lysyl)-L-lysine
                     modification to protein-N6-(beta-lysyl)-L-lysine [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HFO5822323.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPNDPLLRQVLTSQDEFVVAPGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITDALVERFSHSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
BASE COUNT         4471 a         4710 c         4585 g         4112 t
ORIGIN      
        1 ccttggcaag gtcgtgctct accaactgag ctattcccgc gtactactta attttgcttt
       61 cgtaattttc gcatttctgc gtcgttacgg gaggcgcatt atacgagaaa tctttccctc
      121 tgcaaccccc ctaaaaaatt ttttttgagt ttttatctca agtgctgaaa ttatcagcaa
      181 gacgttcaat ttagcgtcaa aatcacgccg ccgcaagtgg attccggcgc ttacgtgacc
      241 aggaaaaatt ttacagcttg ataaaatgct cgcggtagta agccagttct gccaccgact
      301 cacggatatc atccatcgcc tgatgcgtcc cctgcttggt aaaaccatca agaatttccg
      361 gcttccagcg gcgcgccagc tctttcaggg tgctgacatc gagataacgg tagtggaagt
      421 aggcttccag ctccggcata tatttaaaca ggaagcgacg atcctggccg atgctgttac
      481 cgcaaatcgg cgattttccc gcaggcaccc actgttttaa gaattcgagc gttgccagtt
      541 cagcttcacg atcgcccatc gtgctcgctt tcacgcgctc caccagcccg ctggcggtat
      601 gggtgcgcac gttccagtca tccatcagcg ccagctgttc atcagactgg tgtactgcaa
      661 tggtcggccc ttctgccaga atattcaggt tggcatcggt caccagcgtg gcaatctcaa
      721 taatgcgatc gcgctcggga tccagaccgg tcatctcaag atcgatccaa atcaggttgt
      781 tttcattggc actcatgcta ttttccaccc attcacagtg actatattga tccacaattg
      841 cgtgtatcat agatgttttg cccatcaggg gcgaccagga gtctgtacga ttgagtaaaa
      901 ataaactctc caaaggccag cagcgccgcg tgaacgccaa tcaccagcgt cgtcttaaaa
      961 cgtctaagga gaagcccgac tacgacgaca atctgtttgg cgagcctgat gaaggtatcg
     1021 tcatcagccg ctttggtatg cacgctgatg tggaatccgc cgatggcgac gttcaccgct
     1081 gcaatattcg ccgtaccatc cgttcgttgg tgaccggcga ccgcgtagtc tggcgtccgg
     1141 gtaaaccagc ggcggaaggc gtaaatgtca aagggatcgt ggaagcggtg catgagcgca
     1201 cctcagtgtt gacgcgcccg gatttttacg acggtgtgaa acctattgct gccaacatcg
     1261 accagattgt cattgtctcc gccattttgc cggagctgtc gctcaatatt atcgaccgtt
     1321 acctggtggc ctgcgaaacc ttgcagattg agccgattat tgtgctcaac aagatagacc
     1381 tgctggacga cgaaggcatg gcattcgtca acgagcagat ggatatctac cgcaatatcg
     1441 gttatcgcgt attgatggtt tccagtcata ctcaggatgg gctgaaaccg ctggaagagg
     1501 cgttgaccgg gcgcatcagc atttttgccg ggcagtctgg cgttggcaaa tccagcctgc
     1561 tgaacgcatt gctgggtctg caaaaagaga tcctgaccaa cgatgtctct gacaactcgg
     1621 gtctcggtca gcacaccacg accgccgctc ggctgtatca cttcccgcac ggcggtgatg
     1681 tgattgactc cccaggagtg cgtgagttcg gcctctggca cctggagccg gaacaaatca
     1741 ctcagggctt tgtcgaattc catgactact taggtctgtg taaatatcgc gattgcaaac
     1801 acgataccga tccgggctgc gctatccggg aagcggttga ggaaggaaaa atcgcggaaa
     1861 cccgtttcga aaactatcac cgtattctgg aaagcatggc gcaggtaaaa acgcgtaaaa
     1921 acttttctga tacggatgac tgacaattaa gctaaccgtc gttagaatcg tccccttttt
     1981 ttcaggatcc ggcatgtagg ccggatcagg aacgacaaaa caatggcctg gaggctacct
     2041 tgttaaattc atttaaactt tcgctacagt acattctgcc gaaactatgg cttactcgcc
     2101 tggcgggttg gggcgcaagc aagcgggcag gatggctgac aaaactggtt atcgatctgt
     2161 tcgttaaata ctacaaggtc gatatgaaag aggcgcaaaa gccggacacc gccagctacc
     2221 gcacctttaa cgaattcttt gtccgtccgc tgcgtgacga agtacgccca atcgataccg
     2281 atccgaatgt actggttatg cctgccgatg gcgttatcag ccagttgggt aaaatcgaag
     2341 aagataaaat cctgcaagcc aaaggccaca actacagcct cgaagccctg ctggctggca
     2401 actatctgat ggcggacctg ttccgcaacg gtacgtttgt gaccacttac ctctccccac
     2461 gtgactacca ccgcgtacac atgccgtgca acggtattct gcgtgagatg atctacgtgc
     2521 cgggcgatct cttttccgtt aaccatctca ccgctcagaa cgtgccgaat ctgtttgccc
     2581 gtaacgaacg cgtgatctgc ctgttcgata ccgaatttgg cccaatggcg cagattctgg
     2641 tcggggcgac gattgtaggc agcattgaga cggtctgggc gggcaccatt acgccgccgc
     2701 gcgaaggtat catcaagcgc tggacctggc ctgccgggga aaacgacggt tctgtggcac
     2761 tgctgaaagg ccaggaaatg ggtcgcttta aactcggttc caccgttatc aacctgtttg
     2821 caccgggtaa agtgaatctg gttgagcaac tggaaagcct gtctgtcacg aaaattggcc
     2881 agccgctggc agtatctacc gaaacctttg ttacaccaga cgctgaaccg gccccgcttc
     2941 ctgctgaaga gatcgaagca gaacacgacg ccaacccatt ggttgacgac aacaaagacc
     3001 aggtctaatc catcaaagga aacgctgacg tgcgcctgat tatcactttt ctgatggcct
     3061 ggtgcctcag ttggggggcg tacgccgcga cggcccccga tagcaaacaa atctcacaag
     3121 aactggagca ggcaaaagcg gcgaaacccg cgcagccgga agtcgtagag gcgctccagt
     3181 ccgccttaaa tgcgcttgag gaacgaaaag gttcccttga gcgcatcaag caatatcaag
     3241 aagtcattga taattacccg aaactttccg ctactctgcg cgcacaatta aacaacatgc
     3301 gtgacgaacc gcgcagcgtg tcaccgggaa tgtctaccga cgcgctgaat caggaaattc
     3361 tccaggtcag cagccagttg ctggataaaa gccgtcaggc ccagcaagag caggaacgcg
     3421 cccgcgagat tgccgattcg ctgaatcaac tgccgcaaca gcaaaccgat gcccgccgtc
     3481 agttaaatga aatcgagcgc cgcctgggga cgcttaccgg caattccccg ctcaatcagg
     3541 cacaaaattt cgcgttgcag tctgactccg cacgcctgaa agcgctcgtc gatgaactgg
     3601 aactggcgca gctgtctgcc aataaccgtc aggaattagc gcgcttacgc tcagagctgg
     3661 cggaaaaaga gagccagcaa ctggatgcgt atttgcaggc cttacgtaat cagttgaaca
     3721 gccaacgtca gcttgaggcg gagcgggcgc tggaaagtac cgaattactg gcagaaaaca
     3781 gtgccgattt gccgaaagat atcgtcgcgc aattcaaaat taaccgcgaa ctatcggcag
     3841 cgctgaatca acaggcgcag cggatggatc tcgttgcctc gcaacagcgt caggctgcca
     3901 gccagacgtt acaggtccgg caggcgttga atacgctgcg tgaacagtcg caatggctgg
     3961 gctcgtccaa cctgctcggc gaagcgttgc gggcgcaggt ggcacggcta ccggaaatgc
     4021 caaaaccaca acagcttgat accgaaatgg cgcagttgcg tgtgcaacgg ttacgttatg
     4081 aggatctgct aaataaacag ccgctgctac ggcaaatcca tcaggccgac ggtcagccgc
     4141 tgaccgccga gcaaaaccgt attctggaag cacagctacg cactcagcgt gagttgctga
     4201 actcattgtt gcagggtgga gacacgctac tactggaact gaccaagctg aaagtctcca
     4261 acgggcaact ggaggatgcg ctgaaagagg tgaatgaagc gacgcaccgc tatctgttct
     4321 ggacctctga cgtgcgcccg atgaccatcg cctggccgct ggaaatcgcc caggatctgc
     4381 gtcgtctcat ttcgctggac accttcagtc agttgggcaa agccagtgta atgatgctga
     4441 ccagtaaaga gacgattttg ccgctgcttg gcgcgttgat tctggtcggt tgcagtattt
     4501 actcgcgccg ctatttcacc cgttttcttg aacgttcggc ggcgaaagtc ggcaaagtga
     4561 ctcaggatca cttctggctg acgttacgca ctcttttctg gtcgattctc gtcgcgtcac
     4621 cgctgcctgt gctgtggatg acgctgggtt acggcttgcg cgaggcgtgg ccttatccgc
     4681 tggcggtcgc gattggtgat ggcgtaacag ccaccgtgcc gctgctgtgg gtagtgatga
     4741 tttgcgccac ctttgcccgc ccgaacggct tgtttatcgc tcattttggc tggccgcgcg
     4801 aacgggtttc ccgtgggatg cgctactacc tgatgagcat cgggcttatt gtgccgctga
     4861 ttatggcgct gatgatgttc gataacctcg acgaccgtga attctccggt tcgctgggac
     4921 ggctttgctt tatcctcatt tgcggtgcgc tggcggtggt cactctcagt ctgaaaaagg
     4981 ccgggatccc gctgtatctc aacaaagaag gcagcggcga caacattacc aaccatatgc
     5041 tgtggaacat gatgattggc gcaccgctgg ttgccattct ggcgtcggcg gtgggttatc
     5101 tggcaacggc gcaggcgctg ttagcgaggc ttgaaacctc ggttgccatc tggttcctgc
     5161 tactggtggt ttatcacgtt atccgccgct ggatgctgat ccagcgccgc aggctggcgt
     5221 ttgaccgggc gaagcatcgc cgggcagaga tgttagcgca gcgtgcgcgt ggcgaagagg
     5281 aagcgcatca tcacagtagc ccggaaggag caattgaagt cgatgaaagc gaagtcgatc
     5341 tcgatgccat cagtgcgcaa tccttgcggc tggtgcgctc aattttgatg ttgatcgccc
     5401 tgctttcggt cattgtgctg tggtcagaaa tccattccgc tttcggcttc ctcgaaaata
     5461 tttcgctgtg ggatgtcact tccacggtac agggcgtaga aagcctggag ccgattaccc
     5521 tcggtgcggt gctgattgcc attctggtgt ttatcatcac cacgcagctg gtgcgcaacc
     5581 tgcccgcgct gctggaactg gcgattctgc agcacctgga tttaacgccg ggtacgggct
     5641 acgccatcac caccatcacc aaatatctgc tgatgctgat tggcgggctg gtcggcttct
     5701 cgatgattgg tattgagtgg tcgaaattgc agtggctggt cgccgcgctc ggtgttggtc
     5761 tcggttttgg tttgcaggaa attttcgcca actttatctc tggcctgatt atcctgttcg
     5821 aaaaaccgat tcgcattggc gatacggtaa cgatccgcga tcttaccggt agtgtgacga
     5881 aaattaacac ccgcgccacc accatcagcg actgggaccg caaagaaatt atcgtgccga
     5941 acaaggcgtt tattaccgag cagtttatca actggtcgct ctctgactcg gtcacgcgcg
     6001 tggtgttgac gattccggcc cctgccgatg ccaacagtga agaagtgacg gaaatcctgc
     6061 tcaccgcagc gcgtcgctgc tcgctggtaa tcgataaccc agcaccggaa gtcttcctgg
     6121 tggatctgca acaggggatt caaattttcg agctgcgtat ttacgccgct gagatgggtc
     6181 accgtatgcc actacgccat gagattcacc agctaattct ggcaggattc catgcccacg
     6241 gtattgatat gccattcccg cccttccaga tgcgtttgga aagcctgaat ggtaagcaaa
     6301 cggggagaac attaacttct gcgggcaaag gtcgtcaggc gggaagtttg taaggtcttt
     6361 gtggccggat gaacccatcc ggcttataag caaaactgat agcgcagaaa aaactgccac
     6421 agccaatagc cgaaaatagc cagcgcggtg gcgtacataa accagccgcg tttttttcgc
     6481 cacaaagcga accagagaaa agggaaaaag agcggtaacg tcattatcac accgacatca
     6541 cgggtatcgt catcttcgag atacatcagc acatcgcaga cgttattaat cccgcgtcca
     6601 tcaatcatcc actctttgtc cagcgcggaa gtgatggtta tccagaggaa cgccacaata
     6661 aaccagatgc agcacaagat aaacatgcgc gcactcatga gataatcggt cgcttaattt
     6721 cgctatagcg catacgaaac aaatcgccgt atcccactaa ttctccgcga aaaaccagac
     6781 atctaataag ataagcgcaa tagatatccg gcaggtttcc ccaccgcgga ccatcgcgct
     6841 ctttgagggt attaatagtg ttacccacca ccatcgctac tttgcgggtg tggcgggtat
     6901 cgacaaaaga gaggaccgtt tgctctattt ccgccacatc cccggcagaa aattgcagct
     6961 ccctgatttc atcttcggtt atcactggat cccgactagc gtcgtccata acgtcttcct
     7021 ttccagacaa ctcatcgccg gagagacgat gagttgcagg atttcatatg atcagtcgag
     7081 agctaactca ggtttgttcg ccatgcgact gcaatagttc tggtaaatcg ccatcgctaa
     7141 cagcgagaag aagacagggc cgccaatcat ccacaatgtg ctgtcccagt ctccagcttc
     7201 aaccacaggt tgaatgatgg tgaagacgtt cgcaaatgtc accaccagta cgacaaccac
     7261 tgttgcaatc attgccgaca tatgcgtttt gaaaatcaca aacggtctgt cgagatcctg
     7321 acgtgcttta aagaatggga aagccagcgc gaggaacagg taaggaagcg tcatagacac
     7381 gttcgccatc agcgtcagct tgttaaagaa cgccgatgcg gtaccgccac caaacgaaac
     7441 cagcaggatg aagacagtaa ccaacccgca ctgcatccac atggcgatag acggcatccc
     7501 catcgcattc aggcgcgtca tcggttccgg ccacaacgct ttcggcgtcc cctggatgat
     7561 ggctttcagc ggtgaatagc aaagcgtaaa gaacgcaccg gtataagcga ggaacatcga
     7621 aagtccggta atacgcgcaa accatacgcc cagcgacaat gacgcttcag gtgacaaatg
     7681 cagtgcgtta cccagcgtca ccccgaggct cttcatcagc acataggtaa tattgccgag
     7741 gttaacggaa ccattactta atacctgctg ccagtttgtg ctgacgcccc ataaaaatat
     7801 tgccagcgaa taaccgattg aaataacaat agcggcaaaa acaatacctt tggcaaagtt
     7861 cttttctgga ttttccgttt tatcgaccag accaccaacc gcttcaattc caccataggc
     7921 aaaaatagca aataccacaa acgatagcat tgccagaccg gactgataac ctgggttcgg
     7981 tgatgcaagg aaattaatat cctgcgcgaa atgcccacca tttaataaca aaatagtaat
     8041 gcttactaac agcaatacta aattcagaca cattactgca ataccgccca ccgcagtaat
     8101 gcgggcaatt ttattaatcc cttttgaagc aacgacggtg accagaatca tccatgccac
     8161 tgccagcaga ccaaccacct gcgtaggctc cagtccggca atacgccagt gctgggtcat
     8221 gtcgctacca tagaggaatg ttgagaacgg tacccaaact ttcgcggagg tactcaccat
     8281 ccagatgata taagaggaaa accacataaa cgtaccaatg aaggcaaaac gtggtccgac
     8341 actattattc atccaggaat agataccgcc ttcttctttg cgataagcag ctcccatttc
     8401 agccatcatt aaggcgaatg gaataaagaa taacaatgca gaaaatatat aaaagggaat
     8461 cgcactataa cccattaagt aataagccga tgggctattg gcaaatccaa atacggaagt
     8521 aaagatcatc aatatgagtc ctatcagact catctttttt atcgtgtgag gcatgaaaac
     8581 atccttcatt tcagcgcgtg tataaaacgc gcccactgaa ctgcactgcc caacattgag
     8641 cgggcagcgt aaaaaccaat acagaattga tacctggatg atatcaaata tccagtctta
     8701 aattgtggcc tttagcggaa aaatgaaaac atatgctaca gaatggcgct tatcacgcca
     8761 ttcttcgcgg ttaattcagt aatttttcag aattatgccc ggtcaacgct aaaggcgatg
     8821 acttcagcca gtgtctccgc gcccagcgcc aacatcacca gacgatcaac gcctaatgcc
     8881 acgccggaac agtcaggcat accgactttc aaggcttcaa tcagattctg gtcaatgggg
     8941 tgctgcggca aaccgcgcgc cgcgcgctta cggttatctt gttcaaagcg ttgttgctgc
     9001 tcacgggcat ccgtcaattc atggaaacca ttcgccagct caataccttt ataataaacc
     9061 tcaaagcgtt cagcgacccg atgatcttcg gtactgattt gcgccagtga tgcctggctg
     9121 gctggaaagt ggtacacaaa ggtcggtttt tctttgccaa tatttggctc tacaccaaag
     9181 gtaaacagca attgtagaag cgtgtcgcgg tcttcttcag tatcggcaac gttgctcaaa
     9241 tccagtttcg ctgccacttc ccgcagttgc gttttgtcgg cagagagtgg gtcaatttcc
     9301 agataacgca agaaagcttg ttgataagaa aggctttctg ctgccggaca gtccagcacc
     9361 tgttgcaaga gatcgtccac ctcgttcatc aaccggtaca tatcatagtg cggtcgatac
     9421 cactcaagca tagtgaactc agggttgtga taacgcccca tctcttcatt acggaagctg
     9481 cggcacagct ggaataccgg cccacaacca gcaaccagca ggcgtttcat atggtattcc
     9541 gggctggtca ttaaccagag attcatcccc tgcgaatgcc cggggcctac gaaacgtgtc
     9601 tcaaacggga ccaaatgaat atcggttacc gtcgcctggc tcatacaagg cgtctccacc
     9661 tccagcactc cacgatcggc aaagaaacga cggatctccg ccataatcgc cgcgcgtttt
     9721 aataagttag gaatggatgc gctcggctgc caggatgccg tttcgctcat agttaaatct
     9781 ccagtttttg acaagggcac gaagtctact cgcaacgcga cggcgagaca aattttacgc
     9841 aggaatcaaa caagggtggg cagtgactaa aaaaagcacg atctgatggt ttagtaatta
     9901 aattaatcat cttcagtgat aatttagccc tcttgcgcac taaaaaaatc gatctcgtca
     9961 aatttcagac ttatccatca gactatactg ttgtacctat aaaggagcag tggaatagcg
    10021 ttcgcagacc gtaactttca ggcacttacc ctgaagtacg gggctgtggg ataaaaacaa
    10081 tctggaggaa tgtcgtgcaa acctttcaag ccgatcttgc cattgtaggc gccggtggcg
    10141 cgggattacg tgctgcaatt gctgccgcgc aggcaaatcc aaatgcaaaa atcgcactaa
    10201 tctcaaaagt atacccgatg cgtagccata ccgttgctgc agaagggggc tccgccgctg
    10261 tcgcgcagga tcatgacagc ttcgaatatc actttcacga tacagtagcg ggtggcgact
    10321 ggttgtgtga gcaggatgtc gtggattatt tcgtccacca ctgccccacc gaaatgaccc
    10381 aactggaact gtggggatgc ccatggagcc gtcgcccgga tggtagcgtc aacgtacgtc
    10441 gcttcggcgg catgaaaatc gagcgcacct ggttcgccgc cgataagacc ggcttccata
    10501 tgctgcacac gctgttccag acctctctgc aattcccgca gatccagcgt tttgacgaac
    10561 atttcgtact ggatattctg gttgatgatg gtcatgttcg cggcctggta gcaatgaaca
    10621 tgatggaagg cacgctggtg cagatccgtg ctaacgcggt cgttatggct accggcggtg
    10681 cgggtcgcgt ttatcgttac aataccaatg gcggcatcgt taccggtgac ggtatgggta
    10741 tggcgctaag ccacggcgtt ccgctgcgtg acatggaatt cgttcagtat cacccaaccg
    10801 gtctgccagg ttccggtatc ctgatgaccg aaggctgccg cggtgaaggc ggtattctgg
    10861 tcaacaaaaa tggctaccgt tatctgcaag attacggcat gggcccggaa actccgctgg
    10921 gcgagccgaa aaacaaatat atggaactgg gtccacgcga caaagtttct caggccttct
    10981 ggcacgaatg gcgtaaaggc aacactatct ccacgccgcg tggcgatgtg gtttatctcg
    11041 acttgcgtca cctcggcgag aaaaaactgc atgaacgtct gccgttcatc tgcgaactgg
    11101 cgaaagcgta cgttggcgtc gatccggtta aagaaccgat tccggtacgt ccgaccgcac
    11161 actacaccat gggcggtatc gaaaccgatc agaactgtga aacccgcatt aaaggtctgt
    11221 tcgccgtggg tgaatgttcc tctgttggtc tgcacggtgc aaaccgtctg ggttctaact
    11281 ccctggcgga actggtggtc ttcggccgtc tggccggtga acaagcgaca gagcgtgcag
    11341 caactgccgg taatggcaac gaagcggtaa ttgaagcgca ggcagctggc gttgaacaac
    11401 gtttgaaaga tctggttaac caggatggcg gcgaaaactg ggcgaagatc cgcgacgaaa
    11461 tgggcctggc tatggaagaa ggctgcggta tctaccgtac gccggaactg atgcagaaaa
    11521 ccatcgacaa gctggcagag ctgcaggaac gcttcaaacg cgtgcgcatc accgacactt
    11581 ccagcgtgtt caacaccgac ctgctctaca ccattgaact gggccacggt ctgaacgttg
    11641 ctgaatgtat ggcgcactcc gcaatggcac gtaaagagtc ccgcggcgca caccagcgtc
    11701 tggacgaagg ttgcaccgag cgtgacgacg tcaacttcct caaacacacc ctcgccttcc
    11761 gcgatgctga tggcacgact cgcctggagt acagcgacgt gaagattact acgctgccgc
    11821 cagctaaacg cgtttacggt ggcgaagcgg atgcagccga taaggcggaa gcagccaata
    11881 agaaggagaa ggcgaatggc tgagatgaaa aacctgaaaa ttgaggtggt gcgctataac
    11941 ccggaagtcg ataccgcacc gcatagcgca ttctatgaag tgccttatga cgcaactacc
    12001 tcattactgg atgcgctggg ctacatcaaa gacaacctgg caccggacct gagctaccgc
    12061 tggtcctgcc gtatggcgat ttgtggttcc tgcggcatga tggttaacaa cgtgccaaaa
    12121 ctggcatgta aaaccttcct gcgtgattac accgacggta tgaaggttga agcgttagct
    12181 aacttcccga ttgaacgcga tctggtggtc gatatgaccc acttcatcga aagtctggaa
    12241 gcgatcaaac cgtacatcat cggcaactcc cgcaccgcgg atcagggtac taacatccag
    12301 accccggcgc agatggccaa gtatcaccag ttctccggtt gcatcaactg tggtctgtgc
    12361 tacgccgcgt gcccgcagtt tggcctgaac ccagagttca tcggtccggc tgccattacg
    12421 ctggcgcatc gttataacga agatagccgc gaccacggta agaaggagcg tatggcgcag
    12481 ttgaacagcc agaacggcgt atggagctgt actttcgtgg gctactgctc cgaagtctgc
    12541 ccgaaacacg tcgatccggc tgcggccatt cagcagggca aagtagaaag ttcgaaagac
    12601 tttcttatcg cgaccctgaa accacgctaa ggagtgcaac atgacgacta aacgtaaacc
    12661 gtatgtacgg ccaatgacgt ccacctggtg gaaaaaattg ccgttttatc gcttctacat
    12721 gctgcgcgaa ggcacggcgg ttccggctgt gtggttcagc attgaactga ttttcgggct
    12781 gtttgccctg aaaaatggcc cggaagcctg ggcgggattc gtcgactttt tacaaaaccc
    12841 ggttatcgtg atcattaacc tgatcactct ggcggcagcc ctgctgcaca ccaaaacctg
    12901 gtttgagctg gcaccaaaag cggccaatat cattgtaaaa gacgaaaaaa tgggaccaga
    12961 gccaattatc aaaagcctct gggcggtaac tgtggttgcc accatcgtaa tcctgtttgt
    13021 tgccctgtac tggtaaggag cctgagatga ttaatccaaa tccaaagcgt tctgacgaac
    13081 cggtattctg gggcctcttc ggggccggtg gtatgtggag cgcaattatt gcgccggtga
    13141 tgatcctgct ggttggtatt ctgctgccac tggggctgtt tccgggtgac gcactgagct
    13201 acgagcgcgt tctggcgttc gcgcagagct tcattggtcg cgtattcctg ttcctgatga
    13261 tcgttctgcc gctgtggtgt ggtttacacc gtatgcacca cgcgatgcac gatctgaaaa
    13321 tccacgtacc tgcgggcaaa tgggttttct acggtctggc tgctatcctg acagttgtca
    13381 cgctgattgg tgtcgttaca atctaacgca tcgccaatgt aaatccggcc cgcctatggc
    13441 gggccgtttt gtatggaaac cagaccctat gttcaaaatg acgctctgcg ccttattaat
    13501 taccgcctct tgctccacat ttgctgcccc tcaacaaatc aacgatattg tgcatcgcac
    13561 aattaccccg cttatagagc aacaaaagat ccccggtata gcggtggcgg taatttatca
    13621 gggtaaacct tattacttta cctggggcta tgcggacatc gccaaaaagc agcccgtcac
    13681 acagcaaacg ttgtttgagt taggttcggt cagcaaaaca tttacgggcg tgcttggtgg
    13741 cgacgctatt gctcgagggg aaatcaagtt aagcgatccc gcgacaaaat actggcctga
    13801 acttaccgct aaacaatgga atgggatcac actattacat ctcgcgacct acaccgctgg
    13861 cggcctgcca ttgcaggtgc cggatgacgt taaatcctca agcgacttgc tgcgcttcta
    13921 tcaaaactgg caacctgcat gggctccagg aacacaacgt ctgtatgcca acgccagtat
    13981 tgggttgttc ggtgcactgg cggtgaaacc atccggtttg agctttgaac aggcgatgca
    14041 aactcgtgtt ttccagccac tcaaactcac tcatacgtgg attaatgtgc cgtccgcaga
    14101 agaaaagaat tacgcctggg gatatcgcga aggtaaggca gtgcatgttt cgccaggggc
    14161 gttagatgct gaggcttatg gtgtgaagtc gaccattgaa gatatggccc gctgggtgca
    14221 aagcaattta aaaccccttg atatcactga gaaaacgctt caacaaggga tacaactggc
    14281 acaatctcgc tactggcaaa ccggcgatat gtatcagggc ctgggctggg aaatgctgga
    14341 ctggccggtc aatcctgaca tcatcattaa cggcagtgac aataaaattg cactggcagc
    14401 acgccccgta aaagcgatta cgcccccaac tcctgcagta cgcgcatcat gggtacataa
    14461 aacgggggcg accggcggat ttggtagcta tgtcgcgttt attccagaaa aagagctggg
    14521 tatcgtgatg ctggctaaca aaaactatcc caatccagcg agagtcgccg ccgcctgtca
    14581 gattctcaac gctctacagt aaaatttcat cgggtccgaa tttttggacc ttttctccgc
    14641 ttttccttgc tgtcatctac acttagaaaa aaaccagtaa ggaaacattt atgcgcctgc
    14701 tccctctcgt tgccgcagcg acagctgcat ttctggtcgt tgcctgcagt tctcctacgc
    14761 cgccccgtgg cgtgaccgta gtgaataatt tcgatgctaa acgctatctc ggtacctggt
    14821 atgagattgc ccgttttgat caccgttttg aacgtggact ggaaaaagtc accgcaacat
    14881 acagcctgcg tgatgacggc ggcctgaatg tcattaataa aggctataac cctgacagag
    14941 aaatgtggca gcagagtgaa gggaaagcgt actttaccgg cgcaccaact cgcgctgcgc
    15001 tgaaagtgtc attctttggt cctttctatg gcggttataa cgttattgca ctcgatcggg
    15061 aataccgcca tgcgctggtt tgcggtccgg accgcgacta cctgtggata ctctcccgca
    15121 cgccaaccat ttctgacgaa gtgaaacagg agatgctggc agtcgcgacc cgggaagggt
    15181 ttgatgtcag taagtttatt tgggtacagc agcccggtag ttagttagtg agtgctgagt
    15241 ttcagaccaa taatccccaa tacgattaac gccagactcg ccaggcgcat cgggttagcg
    15301 gactcgccga gcagcacaat gccggtgatg gccgcgccga ctgcgccaat acccgtccac
    15361 acggcataag ccgtccctac tggtaacgat ttcatcgccc aggcaagtag cgccatactg
    15421 acaatcatcg ccgtcacagt aataacactt ggcgtcaaac gactaaagcc gtgggtatat
    15481 ttcaggccaa cggcccatac cacttccagc aggccagcaa taactaagat aatccaggac
    15541 atatcaggct ccggagaatt ggggccgtcc ccggtgaaaa gaggcgtttg caggtcgtcc
    15601 tgcaaagctg atggttgaac ggatattttg ccaggtggca ggggaatttc aactattacc
    15661 tttgtgacta tcgcaaaaaa acaaacggca cgacacagct gtcgtaccgt tattgcttac
    15721 tgctgcgctt tcgttgctgc gccagaaatc gcgttaccgc catcagaaat gtcttcacca
    15781 acgccacgcg tggtgttgca agcagttaat actgttgaaa gcaccagaac agaaaagatc
    15841 gctgcaattg tcttcttcac cataacgtct tccttttagg taatgttatt tatgtttgcc
    15901 tatagcaaat taataataga caacatcgcc aaaaatgatg gaaatctgat gatttttcgg
    15961 aggagaagaa aaattagctg gcagcgcggg agatggagtt gccgagatgt ttaatgtctt
    16021 caccaaaacc gcgagcggtg ttacagcccg tgagcagcgc actggcaagc aaaacaagaa
    16081 cgataaggcg tttcatcatc tctgacctga ataagtgatg gtgcagcctg caggccgcac
    16141 cacaaccgca ttacttcacg cgagagacgt attcaccaga gcgggtatcc actttgatga
    16201 cttcgccgat ctgtacgaac agcggaactt taaccacagc gccagtagac agggtagctg
    16261 gtttgccgcc agtacctgcg gtatcacctt tcaggcccgg atcggtatca acgatttcca
    16321 gctcaacgaa gttcggcgga gtaacggaga tcggctgacc attccacaga gttacgatac
    16381 actctgcctg atccagcaac catttggcgt tgtcgccaat tgcttttgca tcagcagaca
    16441 gctgctcgaa agtttcgttg ttcatgaagt gccagaactc accgtcgttg tacaggtaag
    16501 tcaggttcat atcgacaaca tcagcacctt cagcggaatc agtagatttg aaggtttttt
    16561 ctacgcgagt accggtcagc agacgacgca gtttaacgcg agcaaatgcc tggcctttac
    16621 ccggttttac gaattcactc gcttcaaccg cgtaaggttc gccgtctaac atgattttaa
    16681 gaccagcacg aaaatcgttg ctatagtacg ttgccataag gccctctgaa atttgttaat
    16741 tggtagctaa gccacaaaat ggcgcatatt gtaaccctaa ataccccatc cagagaagat
    16801 tggttaacgc aacttgccga tgttgtgacc gatcctgatg aacttctgcg tcttttgaat
    16861 atagacgcgg acgaaaaact gttggccggg cgcagcgcca aaaagctgtt tgccctgcgt
    16921 gtgccccgct catttatcga tcgcatggag aaaggcaatc cgaacgatcc gcttttgcgt
    16981 caggtactta cctcgcaaga tgagtttgtt gtcgcgcccg gattctccac cgacccgctg
    17041 gaagaacagc acagcgtagt gccaggtttg ttgcataaat accacaaccg ggcgcttttg
    17101 ctggtcaaag gcggctgcgc ggtaaattgc cgctattgct tccgtcgcca cttcccctat
    17161 gccgaaaatc agggcaacaa gcgtaactgg cagactgcac ttgagtatgt tgctgcgcat
    17221 ccggaactgg acgagatgat tttctccggc ggcgatccgc tgatggcgaa agatcacgag
    17281 ctggactggt tgctcacaca actggaagcc atcccgcata tcaaacgtct gcggattcac
    17341 agccgtctgc cgattgtgat cccggcacgc atcacagatg cgctggttga acgcttttct
    17401 cattctacgc tgcaaatctt gctggtgaat cacattaacc atgccaatga ggtagatgaa
    17461 acattccgtc aggcgatggc taagttgcgc cgggtaggcg ttactttgct caaccagagc
    17521 gttctgttac gtggtgtgaa cgataacgcg caaacgctgg caaacctgag taatgcgttg
    17581 ttcgatgcgg gcgtgatgcc gtattacctg catgtgctcg ataaagtaca gggcgcggcg
    17641 cattttatgg tgagtgatga cgaagcacgg cagattatgc gtgagttgct gacactggtg
    17701 tcgggatatc tggtgcctaa actggcgcga gaaattggcg gcgaacccag caaaacgccg
    17761 ctggatctcc agctacgcca gcagtaactg attaaacagg gaagtttaat ctttgcacgt
    17821 tgcataacct ttaattattc atatacattg ctgttattac tttttatata accaacac
//
LOCUS       DAZFLD010000895         5482 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.guided.251, whole genome shotgun sequence.
ACCESSION   DAZFLD010000895 DAZFLD010000000
VERSION     DAZFLD010000895.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5482
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.guided.251"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            120..326
                     /locus_tag="ACHKBL_005645"
                     /pseudo
     CDS             120..326
                     /locus_tag="ACHKBL_005645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
     gene            322..674
                     /gene="prfH"
                     /locus_tag="ACHKBL_005646"
                     /pseudo
     CDS             322..674
                     /gene="prfH"
                     /locus_tag="ACHKBL_005646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="similar to release factor 1 and 2; frameshifted;
                     incomplete; partial in the middle of a contig; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
     gene            complement(731..2188)
                     /gene="pepD"
                     /locus_tag="ACHKBL_005647"
     CDS             complement(731..2188)
                     /gene="pepD"
                     /locus_tag="ACHKBL_005647"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HFO5822324.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            2449..2907
                     /gene="gpt"
                     /locus_tag="ACHKBL_005648"
     CDS             2449..2907
                     /gene="gpt"
                     /locus_tag="ACHKBL_005648"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFO5822325.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQNTWIEQPWDMGVVFVPPISGR"
     gene            2999..4243
                     /gene="frsA"
                     /locus_tag="ACHKBL_005649"
     CDS             2999..4243
                     /gene="frsA"
                     /locus_tag="ACHKBL_005649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HFO5822326.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFSHGAQLPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACAEEDPQLSGRYWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKVVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITDWIEKRLC"
     gene            4301..4702
                     /gene="crl"
                     /locus_tag="ACHKBL_005650"
     CDS             4301..4702
                     /gene="crl"
                     /locus_tag="ACHKBL_005650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HFO5822327.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWTSVQIKDAEVVERLEHTLRE
                     FHEKLRELLATLNLKLEPADDFRDEPVKLTA"
     gene            complement(4741..>5482)
                     /locus_tag="ACHKBL_005651"
     CDS             complement(4741..>5482)
                     /locus_tag="ACHKBL_005651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="porin"
                     /protein_id="HFO5822328.1"
                     /translation="DLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRAS
                     GLATYRNTDFFGVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGA
                     YTNSDRTNEQNLQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKT
                     QNFEAVAQYQFDFGLRPSLGYVLSKGKDIEGIGDEDLVNYVDVGATYYFNKNMSAFVD
                     YKINQLESDNKLNINNDDIVAVGMTYQF"
BASE COUNT         1336 a         1347 c         1416 g         1383 t
ORIGIN      
        1 tgcaggaagg cgacaagcga gtgaatcccc aggagcttac taaagtaagt gactggggtg
       61 aacgaacgcg gtcgcagtac atgcaacttg aagtatgacg agtataaatg gaatgataca
      121 tgggcaaata tattcgtccc ttatccgatg cggtatttac catcgcatct gatgacctgt
      181 ggatcgagag tttagcgatc caacaattac acaccacggc aaatttaccc aacatgcagc
      241 gcgttgtggg gatgccagat ttacatcccg gacgcggcta cccgattggc gcagcgttct
      301 tctctgttgg tcgtttttac ccggcaagac gtcgcggtaa cggtgctgga aacagaaacg
      361 ggccgctact ctgacacgct gcgttcggcg ctgatttctc tggatggcga taatgcatgg
      421 gcattaagcg aaagttggtg cggcactatt cagtgtattt gtccgagtcc gtatcggcct
      481 catcatgggc gcaaaaactg gtttctgggc atgctaacaa gcggctggcg cgattgctga
      541 ttgcctggaa gctggaacaa cagcaacagg aaaatagcgc ggcgctgaaa tcgcagcggc
      601 gaatgttcca tcaccagatt gaacgtggca acccgcgacg aacgttcaca gggatggctt
      661 ttatcgaagg ataatgaagg atgaaactgc cggaaggcga ttaaacgcca tccggcagca
      721 aatcaaataa ttacttcgcc ggaatttctt tcagcagttc agtcagtagt gtccagtaat
      781 gacctacgct ttcgatgtga acttgctcat ccggagagtg tggaccggtg atggttggcc
      841 cgatagaaac catgtccatt tccggatacg gttttttgaa cagaccacat tccaggcccg
      901 cgtggataat ctggatgttc ggcgtcttgt tgaacaggcg ctgataggtt tcacgtacca
      961 gatgcatcac cggagaatta gcgtccggct gccagccagg atatgcgcct ttcgcttcgg
     1021 ttttcgcgcc agccagttta cccagcgaat ccagcatgct caccacgtag tctttaccgc
     1081 tgtcgatcag agaacggatc aggcagtgaa tttctacgtt attgtcagtc atggtcacca
     1141 caccgacgtt cagggaggtt tcgaccacac cttttgccac gtcggagttg cggatcacac
     1201 cgttcggggt ggcgttcagc agacgaataa aggtatcgcg agatttcgca atcagggcag
     1261 ctttatcgtt cgcaacagag tccagcaaca aggccagatt tttctctttc tctgccagct
     1321 cgtttttcag gatctcctga taggtattca ccagagattt caaagcgtcg actttatcag
     1381 ctgcgacagc aatggtcgca aaggcttcac gcgggatggc gttacgcagt gtgccgccgt
     1441 tgaaatcgat aaggcgcagg tccagttctt ctgcatgacc cgccaggaag cgcaccagca
     1501 gtttgttggc attacccagg ccaacgtgga tttcaccgcc ggagtgaccg cctttcaggc
     1561 cttttaaggt taacttgaag gtttcaaagc cagctggaac cgcttcacga tctaaatgca
     1621 ggttggaggt gaagtcgata cctcccgcac aacccatgta gatttcacct tcttcttcgg
     1681 agtcggtgtt aatcagaata tcagcctgca accagttgct ctgtaagcca aaagcaccgt
     1741 ccataccggc ttcttcggtc atggtcagca gcacttccag cgggccgtga accacgtttt
     1801 cgtcagccag aaccgccagt gcagaggcca taccaatgcc gttatccgca cccagcgtgg
     1861 tgccgcgtgc tttaacccat tcgccatcaa tataaggctg gataggatct ttggtgaagt
     1921 catgcacggt gtcgttattt ttttgcggca ccatatcgag gtgggcctgc aagacgaccg
     1981 gtttacgatt ttccatacct gcggtagcag gtttacgaat caggatatta cctacctgat
     2041 cgcgttcgac atggaaacct ttctcttttg cccaaccaac aatgtattca gcgagttgct
     2101 cttcatgata ggacgggtga ggaatagaac agattttggc aaaaatatcc cacagcggct
     2161 gtggagataa ttgagacagt tcagacacgt taagtctcct tgtcgatcac ccgcaaaaca
     2221 gtattgcagg tcacagggtt agcagaaaat gttgtcaaca caagacaggc ttgcgagata
     2281 tgtttgagaa taccacttta tcccgcgtca gggagaggca gtgcgtaaaa agacgcggac
     2341 tcatgtgaaa tactggtttt tagtgcgcca gatctctata atctcgcgca acctattttc
     2401 ccctcgaaca ctttttaagc cgtagataaa caggctggga cacttcacat gagcgaaaaa
     2461 tacatcgtca cctgggacat gttgcagatc catgcacgta aactcgcaag ccgactgatg
     2521 ccttctgaac aatggaaagg cattattgcc gtaagccgtg gcggtctggt accgggtgcg
     2581 ttactggcgc gtgaactggg tattcgtcat gtcgataccg tttgtatttc cagctacgat
     2641 cacgacaacc agcgcgagct taaagtgctg aaacgcgcag aaggcgatgg cgaaggcttc
     2701 atcgttattg atgacctggt agataccggt ggtactgcgg ttgcgattcg tgaaatgtat
     2761 ccaaaagcgc actttgtcac catcttcgca aaaccggctg gtcgtccgct ggttgatgat
     2821 tatgttgttg atatcccgca aaatacctgg atcgaacagc cgtgggatat gggcgtcgta
     2881 ttcgtgccgc caatctccgg tcgctaatct tttcaacgcc tggcactgcc gggcgttgtt
     2941 ctttttaact tcaggcgggt tacaatagtt tccagtaagt attctggagg ctgcatccat
     3001 gacacaggca aacctgagcg aaaccctgtt caaaccccgc tttaaacatc ctgaaacctc
     3061 gacgctagtc cgccgcttta gtcacggcgc acaactgcct gtgcagtcgg cccttgatgg
     3121 taaaaccatc cctcactggt atcgcatgat taaccgtctg atgtggatct ggcgcggcat
     3181 tgacccccgt gaaatcctcg acgtccaggc gcgtattgtg atgagcgatg ccgaacgtac
     3241 cgacgatgat ttatacgata cggtgattgg ctaccgcggc ggcaactgga tttatgagtg
     3301 ggctacccag gcgatggtgt ggcaacaaaa agcctgtgcg gaagaagatc cgcaactcag
     3361 cggtcgatac tggctgcatg cggctacgtt gtacaacatt gctgcctatc ctcatctgaa
     3421 aggagatgac ctggccgagc aagcgcaggc tttgtcaaac cgcgcctatg aagaggccgc
     3481 tcagcgtctg ccgggcacga tgcggcagat ggagtttacc gtacccggcg gtgcgcccat
     3541 caccggcttt ttgcatatgc cgaaaggcga tggcccgttc ccgacagtat taatgtgtgg
     3601 tggtctggat gcgatgcaga cggactatta cagcctgtat gaacgttatt ttgcgccgcg
     3661 cggcattgct atgctgacta ttgatatgcc gtcggtgggc ttttcttcaa aatggaagct
     3721 cacccaggac tccagcctgt tgcatcagca cgttttaaag gcgctgccta acgtaccgtg
     3781 ggtggatcac actcgcgtcg cggcctttgg tttccgtttc ggcgctaacg ttgccgtgcg
     3841 tctggcatac cttgaatcgc cgcgtctgaa agtggttgcc tgtcttggtc cggtagttca
     3901 taccctgttg agtgatttta agtgccagca acaggtgccg gaaatgtatc ttgacgttct
     3961 ggccagtcgt ttagggatgc atgatgcttc cgatgaagcg ttgcgagtgg agttgaatcg
     4021 ctattcatta aaagtgcaag gattgctggg acgtcgctgc ccaacgccaa tgttatcagg
     4081 ctactggaag aacgatccgt tcagcccgga agaggactca cgcttaatca cgtcgtcatc
     4141 tgctgacggt aaattattag agatcccatt taacccggtg tatcggaatt ttgacaaagg
     4201 ccttcaggaa atcacagact ggatcgaaaa acgtttgtgt taaaaatttg ctaaatttta
     4261 ccaatttggt aaaacagttg catcacaaca ggagattgca atgacgttac cgagtggaca
     4321 cccgaagagc agattgatca aaaaatttac cgcactaggc ccgtatattc gtgaaggtaa
     4381 gtgcgaagat aatcgattct ttttcgattg tctggctgta tgcgtcaacg tgaaaccggc
     4441 accggaagtg cgtgagttct ggggctggtg gatggaactg gaagcgcagg aatcccgttt
     4501 tacctacagt taccagtttg gtctgttcga taaagcaggc gactggacga gtgttcagat
     4561 aaaagatgcc gaagtggttg aacgactgga gcacaccctg cgtgaatttc acgagaagct
     4621 gcgtgagctg ctggcgacgc tgaatctgaa gctggaaccg gcagatgatt ttcgtgatga
     4681 accggtgaag ttaacggcgt gaatgaaatg tgccggatgc atcacatccg gcaatattca
     4741 ttaaaactga tacgtcatcc caaccgcgac aatatcatca ttattaatat ttaatttgtt
     4801 atcgctttcc agttggttga ttttataatc aacaaacgct gacatatttt tgttgaaata
     4861 atacgtagca ccaacatcga cataattgac cagatcttca tcaccgatgc cctcaatatc
     4921 tttccctttc gataagacat aacccagcga tggacgcaga ccaaagtcaa actggtattg
     4981 agcgaccgct tcaaagttct gcgtcttatt ggcaaagccg ccagttattg gcgtcatttt
     5041 gcgtgtttca gaatagaaag ttgccagata aatattattg gcatcgtatt tcagacctgt
     5101 tgcccatgct tctgcacgct tgcctgtgcc acggctttgc aggttctgct cgttggtgcg
     5161 atcagagttg gtataggctc cactgatggc gaaatcgctg ccaccaaagt catatgtcaa
     5221 tgacgtgccg aagccatcgc cgttttgctt tttaacgtcg cggttttcgt ttttcccttg
     5281 atattgcagg gttaagttca ggccatcgat aacgccgaag aagtcggtgt tccgatacgt
     5341 cgccagaccg ctggcgcgtt tggtcataaa gttgtcggtc tgcgccgagg agtcgccacc
     5401 aaattccggg aacatatcgg tccaggcttc cacgtcatac aacgcgccca ggttacgacc
     5461 ataatcgaaa gaacccaaat ct
//
LOCUS       DAZFLD010000896         1585 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1901F75122
            SAMN43251777-rid23065823.guided.453, whole genome shotgun sequence.
ACCESSION   DAZFLD010000896 DAZFLD010000000
VERSION     DAZFLD010000896.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251777
            Sequence Read Archive: SRR30293524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350344.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350344.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:46:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 115 of 174
            Pseudo Genes (internal stop)      :: 43 of 174
            Pseudo Genes (multiple problems)  :: 48 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1585
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251777-rid23065823.guided.453"
                     /serotype="O146:H28"
                     /isolate="1901F75122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            132..1091
                     /gene="stxA2b"
                     /locus_tag="ACHKBL_005652"
     CDS             132..1091
                     /gene="stxA2b"
                     /locus_tag="ACHKBL_005652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2b subunit A"
                     /protein_id="HFO5822329.1"
                     /translation="MKCILLKWVLCLLLGFSSVSYSREFTIDFSTQQSYVSSLNSIRT
                     EISTPLEHISQGTTSVSVINHTPPGSYFAVDIRGLDVYQARFDHLRLIIEQNNLYVAG
                     FVNTATNTFYRFSDFAHISVPGVTTVSMTTDSSYTTLQRVAALERSGMQISRHSLVSS
                     YLALMEFSGNAMTRDASRAVLRFVTVTAEALRFRQIQREFRLALSETAPVYTMTPEEV
                     DLTLNWGRISNVLPEFRGEGGVRVGRISFNNISAILGTVAVILNCHHQGARSVRAVNE
                     EIQPECQITGDRPVIRINNTLWESNTAAAFLNRRAHSLNTSGE"
     gene            1104..1367
                     /gene="stxB2b"
                     /locus_tag="ACHKBL_005653"
     CDS             1104..1367
                     /gene="stxB2b"
                     /locus_tag="ACHKBL_005653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000719322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2b subunit B"
                     /protein_id="HFO5822330.1"
                     /translation="MKKIFVAALFAFVSVNAMAADCAKGKIEFSKYNENDTFTVKVAG
                     KEYWTNRWNLQPLLQSAQLTGMTVTIKSNTCASGSGFAEVQFN"
BASE COUNT          427 a          323 c          382 g          453 t
ORIGIN      
        1 aagcaatcgg tcactggttc gaatccagta caacgcgcca tacttatttt ttctggctcg
       61 cttttgcggg ccttttttat atctgcgccg ggtctggtgc tgattacttc agccaaaagg
      121 aatacctgta tatgaagtgt atattgttaa aatgggtact gtgcctgtta ctgggctttt
      181 cttcggtatc ctattcccgg gaatttacga tagacttttc gacccaacaa agttatgtct
      241 cttcgttaaa tagtatacgg acagaaatat cgaccccact tgaacatata tctcagggga
      301 ccacatcggt gtctgttatt aaccacaccc caccgggcag ttattttgct gtggatatac
      361 gagggcttga tgtctatcag gcgcgttttg accatcttcg tctgattatt gagcaaaata
      421 atttatatgt ggccggattc gttaatacgg caacaaatac tttctacagg ttttcagatt
      481 ttgcacatat atcagtgccc ggtgtgacaa ctgtttccat gacaacggac agcagttata
      541 ccactctgca gcgtgtcgca gcgctggaac gttccggaat gcaaatcagt cgtcactcac
      601 tggtttcatc atatctggcg ttaatggagt ttagtggaaa tgccatgacc agagatgcat
      661 ccagagcagt tctgcgtttt gtcactgtca cagcagaagc cttacggttc aggcaaatac
      721 agagagaatt tcgtctggca ctgtctgaaa ctgctcctgt ttatacgatg acaccggaag
      781 aagtggacct cacactgaac tgggggagaa tcagcaatgt gcttccggag tttcggggag
      841 agggtggtgt cagagtgggg cgaatatcct ttaataatat atcagcgata ctgggcacag
      901 tggcggttat actgaattgc catcatcagg gggcgcgttc cgttcgcgcc gtgaatgaag
      961 agatacaacc agaatgtcag ataactggcg acaggccagt tataaggata aacaatacgt
     1021 tatgggaaag taataccgca gctgcttttc tgaatcgcag ggctcactct ttaaatacat
     1081 ccggagaata acaggagtta aatatgaaga agatatttgt agcggcttta tttgcttttg
     1141 tttctgttaa tgcaatggca gctgattgtg caaaaggtaa aattgagttc tctaagtata
     1201 atgagaatga tacattcaca gtaaaagtgg ccgggaaaga gtactggact aaccgctgga
     1261 atctgcaacc gctactgcaa agcgcacagt taacaggaat gacggtaaca atcaaatcaa
     1321 atacctgtgc gtcaggttca ggatttgctg aagtgcagtt taattaatat cagaagcatt
     1381 gctggtttcg tggtgtgcag caatgtagtt acagtgtaat caatgtcaca attcagtcag
     1441 ttgaaggttg tctgcccgac tgagaatttg ttaaaaaaat cctgcatggt gaatccccct
     1501 gagcggaggg gcatatcagc gtcacaggtg tttctgtttt acctctatcc tttctgtgcg
     1561 ggttcaggtg ctgatactga actca
//
